LOCUS       DAZGQL010000001       109696 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.001, whole genome shotgun sequence.
ACCESSION   DAZGQL010000001 DAZGQL010000000
VERSION     DAZGQL010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 109696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 109696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..109696
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.001"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            244..579
                     /gene="yjdM"
                     /locus_tag="ACHKIR_000001"
     CDS             244..579
                     /gene="yjdM"
                     /locus_tag="ACHKIR_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFO9987817.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            739..1182
                     /gene="yjdN"
                     /locus_tag="ACHKIR_000002"
     CDS             739..1182
                     /gene="yjdN"
                     /locus_tag="ACHKIR_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HFO9987818.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTH"
     gene            1315..2103
                     /gene="phnC"
                     /locus_tag="ACHKIR_000003"
     CDS             1315..2103
                     /gene="phnC"
                     /locus_tag="ACHKIR_000003"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFO9987819.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSYFTREQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRVEENAKAA"
     gene            2128..3144
                     /gene="phnD"
                     /locus_tag="ACHKIR_000004"
     CDS             2128..3144
                     /gene="phnD"
                     /locus_tag="ACHKIR_000004"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO9987820.1"
                     /translation="MNAKIIASLTFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEMQGVKGNKGLNEQDKLAKTSAIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            3250..4029
                     /gene="phnE"
                     /locus_tag="ACHKIR_000005"
     CDS             3250..4029
                     /gene="phnE"
                     /locus_tag="ACHKIR_000005"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFO9987821.1"
                     /translation="MQTITIAPPKRSWFSLLSWAIVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            4050..4775
                     /gene="phnF"
                     /locus_tag="ACHKIR_000006"
     CDS             4050..4775
                     /gene="phnF"
                     /locus_tag="ACHKIR_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HFO9987822.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     HFDSGSLHDFLREQTGITLRRSQTRISARRAQAKECQRLEIPNLSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            4776..5228
                     /gene="phnG"
                     /locus_tag="ACHKIR_000007"
     CDS             4776..5228
                     /gene="phnG"
                     /locus_tag="ACHKIR_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HFO9987823.1"
                     /translation="MHADTATRQHWMSVLAHSQPTELAARLNALNITADYEVIRAAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQTRHFQNLSETLIAPLNADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            5225..5809
                     /gene="phnH"
                     /locus_tag="ACHKIR_000008"
     CDS             5225..5809
                     /gene="phnH"
                     /locus_tag="ACHKIR_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HFO9987824.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLSAPLSNDIVSQSLRFHTNAPLVNQPELATFAVTDEAIS
                     SEQLNALSSGTAVAPEAGATLILQVVSLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            5809..6873
                     /gene="phnI"
                     /locus_tag="ACHKIR_000009"
     CDS             5809..6873
                     /gene="phnI"
                     /locus_tag="ACHKIR_000009"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HFO9987825.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGNTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVQEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            6866..7711
                     /gene="phnJ"
                     /locus_tag="ACHKIR_000010"
     CDS             6866..7711
                     /gene="phnJ"
                     /locus_tag="ACHKIR_000010"
                     /EC_number="4.7.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HFO9987826.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     LIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMNMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWNEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKSQ"
     gene            7708..8466
                     /gene="phnK"
                     /locus_tag="ACHKIR_000011"
     CDS             7708..8466
                     /gene="phnK"
                     /locus_tag="ACHKIR_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HFO9987827.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIRYENRSLYGMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            8577..9257
                     /gene="phnL"
                     /locus_tag="ACHKIR_000012"
     CDS             8577..9257
                     /gene="phnL"
                     /locus_tag="ACHKIR_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HFO9987828.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLQRASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGEEWVDLVTAPARKVVEIRKTTIGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIREAKARGAAIVGIFHDEAVRNDVADR
                     LHPMGVSS"
     gene            9254..10390
                     /gene="phnM"
                     /locus_tag="ACHKIR_000013"
     CDS             9254..10390
                     /gene="phnM"
                     /locus_tag="ACHKIR_000013"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HFO9987829.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGKIRAFAESQSRQPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINAIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTEAQMQQYEEEQLALAARWSQPNRETIAAM
                     CRARHIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESNRFTLPQAVKLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRKGNHIHIDHVWRQGKRVF"
     gene            10390..10947
                     /gene="phnN"
                     /locus_tag="ACHKIR_000014"
     CDS             10390..10947
                     /gene="phnN"
                     /locus_tag="ACHKIR_000014"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033863 - ribose 1,5-bisphosphate
                     phosphokinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HFO9987830.1"
                     /translation="MTGKLIWLMGASGSGKDSLLTELRQREQTQLLVAHRYITRAASA
                     GSENHIALSEQEFFTRAGQNLLALSWHANGLYYGVGVEIDLWLHAGFDVVVNGSRAHL
                     PQARARYQSALLPVCLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCLTLNN
                     DGSLRQSVDKLLTLIHQKEKHHACL"
     gene            10934..11368
                     /gene="phnO"
                     /locus_tag="ACHKIR_000015"
     CDS             10934..11368
                     /gene="phnO"
                     /locus_tag="ACHKIR_000015"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HFO9987831.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHQAFRVGFNANLRD
                     PNMRYQLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKVL"
     gene            11370..12128
                     /gene="phnP"
                     /locus_tag="ACHKIR_000016"
     CDS             11370..12128
                     /gene="phnP"
                     /locus_tag="ACHKIR_000016"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HFO9987832.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECVACVRARRSPQYRRQPCSGVV
                     KFNDAITLIDAGRHDLADRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDQIPVYGPP
                     DEQGCDDLFKHSGLLDFSHTVEPFVMFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLRNNQPQVMVIDCSHPPREDAPRNHCDLNTVLALNQVIRSPRV
                     ILTHISHQFDAWLMENALPSGFEAGFDGMEIEVA"
     gene            complement(12156..12485)
                     /gene="yjdP"
                     /locus_tag="ACHKIR_000017"
     CDS             complement(12156..12485)
                     /gene="yjdP"
                     /locus_tag="ACHKIR_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDRRRQL repeat protein YjdP"
                     /protein_id="HFO9987833.1"
                     /translation="MKRFPLFLLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(12554..13003)
                     /gene="rpiB"
                     /locus_tag="ACHKIR_000018"
                     /gene_synonym="alsI"
     CDS             complement(12554..13003)
                     /gene="rpiB"
                     /locus_tag="ACHKIR_000018"
                     /gene_synonym="alsI"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional allose-6-phosphate
                     isomerase/ribose-5-phosphate isomerase RpiB"
                     /protein_id="HFO9987834.1"
                     /translation="MKKIAFGCDHVGFILKHEIVAHLVERGVEVIDKGTWSSERTDYP
                     HYASHVALAVAGGEADGGILICGTGVGISIAANKFAGIRAVVCSEPYSAQLSRQHNDT
                     NVLAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQRRN"
     gene            13362..14252
                     /gene="alsR"
                     /locus_tag="ACHKIR_000019"
     CDS             13362..14252
                     /gene="alsR"
                     /locus_tag="ACHKIR_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose utilization transcriptional regulator
                     AlsR"
                     /protein_id="HFO9987835.1"
                     /translation="MSQSEFDSALPNGIGLAPYLRMKQEGMTENESRIVEWLLKPGNL
                     SCAPAIKDVAEALAVSEAMIVKVSKLLGFSGFRNLRSALEDYFSQSEQVLPSELAFDE
                     APQDVVNKVFNITLRTIMEGQSIVNVDEIHRAARFFYQARQRDLYGAGGSNAICADVQ
                     HKFLRIGVRCQAYPDAHIMMMSASLLQEGDVVLVVTHSGRTSDVKAAVELAKKNGAKI
                     ICITHSYHSPIAKLADYIICSPAPETPLLGRNASARILQLTLLDAFFVSVAQLNIEQA
                     NINMQKTGAIVDFFSPGALK"
     gene            14311..15246
                     /gene="alsB"
                     /locus_tag="ACHKIR_000020"
     CDS             14311..15246
                     /gene="alsB"
                     /locus_tag="ACHKIR_000020"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose transporter substrate-binding protein"
                     /protein_id="HFO9987836.1"
                     /translation="MNKYLKYFSGTLVGLMLSTSAFAAAEYAVVLKTLSNPFWVDMKK
                     GIEDEAKTLGVSVDIFASPSEGDFQSQLQLFEDLSNKNYKGIAFAPLSSVNLVMPVAR
                     AWKKGIYLVNLDEKIDMDNLKKAGGNVEGFVTTDNVAVGAKGASFIIDKLGAEGGEVA
                     IIEGKAGNASGETRRNGATEAFKKASQIKLVASQPADWDRIKALDVATNVLQRNPNIK
                     AIYCANDTMAMGVAQAVANAGKTGKVLVVGTDGIPEARKMVEAGQMTATVAQNPADIG
                     ATGLKLMVDAEKSGKVIPLDKAPEFKLVDSILVTQ"
     gene            15374..16906
                     /gene="alsA"
                     /locus_tag="ACHKIR_000021"
     CDS             15374..16906
                     /gene="alsA"
                     /locus_tag="ACHKIR_000021"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose ABC transporter ATP-binding protein
                     AlsA"
                     /protein_id="HFO9987837.1"
                     /translation="MATPYISMAGIGKSFGPVHALKSVNLTVYPGEIHALLGENGAGK
                     STLMKVLSGIHEPTKGTITINNINYNKLDHKLAAQLGIGIIYQELSVIDELTVLENLY
                     IGRHLTKKICGVNIIDWREMRVRAAMMLLRVGLKVDLDEKVANLSISHKQMLEIAKTL
                     MLDAKVIIMDEPTSSLTNKEVDYLFLIMNQLRKEGTAIVYISHKLAEIRRICDRYTVM
                     KDGSSVCSGMVSDVSNDDIVRLMVGRELQNRFNAMKENVSNLAHDTVFEVRNVTSRDR
                     KKVRDISFSVCRGEILGFAGLVGSGRTELMNCLFGVDKRAGGEIRLNGKDISPRSPLD
                     AVKKGMAYITESRRDNGFFPNFSIAQNIAISRSLKDGGYKGAMGLFHEVDEQRTAEAQ
                     RELLALKCHSVNQNITELSGGNQQKVLISKWLCCHPEVIIFDEPTRGIDVGAKAEIYK
                     VMRQLADDGKVILMVSSELPEIITVCDRIAVFCEGRLTQILTNRDDMSEEEIMAWALP
                     HE"
     gene            16885..17865
                     /gene="alsC"
                     /locus_tag="ACHKIR_000022"
     CDS             16885..17865
                     /gene="alsC"
                     /locus_tag="ACHKIR_000022"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose ABC transporter permease"
                     /protein_id="HFO9987838.1"
                     /translation="MGFTTRVKSEASEKKPFNFALFWDKYGTFFILAIIVAIFGSLSP
                     EYFLTTNNITQIFVQSSVTVLIGMGEFFAILVAGIDLSVGAILALSGMVTAKLMLAGI
                     DPFLAALIGGVLVGGALGAINGCLVNWTGLHPFIITLGTNAIFRGITLVISDANSVYG
                     FSFDFVNFFAASVIGIPVPVIFSLIVALILWFLTTRMRLGRNIYALGGNKNSAFYSGI
                     DVKFHILVVFIISGVCAGLAGVVSTARLGAAEPLAGMGFETYAIASAIIGGTSFFGGK
                     GRIFSVVIGGLIIGTINNGLNILQVQTYYQLVVMGGLIIAAVALDRLISK"
     gene            17876..18571
                     /gene="alsE"
                     /locus_tag="ACHKIR_000023"
     CDS             17876..18571
                     /gene="alsE"
                     /locus_tag="ACHKIR_000023"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allulose-6-phosphate 3-epimerase"
                     /protein_id="HFO9987839.1"
                     /translation="MKISPSLMCMDLLKFKEQIEFIDNHADYFHIDIMDGHFVPNLTL
                     SPFFVSQVKKLASKPLDCHLMVTRPQDYIAQLARAGADFITLHPETINGQAFRLIDEI
                     RRHGMKVGLILNPETPVEAMKYYIHKADKITVMTVDPGFAGQPFIPEMLDKLAELKAW
                     REREGLEYEIEVDGSCNQATYEKLMAAGADVFIVGTSGLFNHAENIDEAWRIMTAQIL
                     AAKSEVQPHAKTA"
     gene            18555..19484
                     /gene="alsK"
                     /locus_tag="ACHKIR_000024"
     CDS             18555..19484
                     /gene="alsK"
                     /locus_tag="ACHKIR_000024"
                     /EC_number="2.7.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0019316 - D-allose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allose kinase"
                     /protein_id="HFO9987840.1"
                     /translation="MQKQHNVVAGVDMGATHIRFCLRTAEGETLHCEKKRTAEVIAPD
                     LVSGIGEMIDEQLRRFNARCHGLVMGFPALVSKDKRTIISTPNLPLTAADLYDLADKL
                     ENTLNCPVEFSRDVNLQLSWDVVENRLTQQLVLAAYLGTGMGFAVWMNGAPWTGAHGV
                     AGELGHIPLGDMTQHCACGNSGCLETNCSGMALRRWYEQQPRNYPLSDLFVHAENAPF
                     VQSLLENAARAIATSINLFDPDAVILGGGVMDMPAFPRETLIAMTQKYLRRPLPYQVV
                     RFIAASSSDFNGAQGAAILAHQRFLPQSCAKVP"
     gene            19757..21742
                     /gene="yjcS"
                     /locus_tag="ACHKIR_000025"
     CDS             19757..21742
                     /gene="yjcS"
                     /locus_tag="ACHKIR_000025"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HFO9987841.1"
                     /translation="MNNSRLFRLSRIVIALTAASSMMVNTANATDEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKIYYRANDYKFDINAAAPETI
                     NPSLWRQSQINGISGLFKVTDKMYQVRGQDISNITFVEGEKGIIVIDPLVTPPAAKAA
                     LDLYFQHRPQKPIVAVIYTHSHADHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLPHNAQGNVGNGLGVTLATGDPSIIAPTKTIVRTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVQALGGSARVINLAKEANKQGDYRWSAELLKQVIAANPGDQVAKNLQADNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTTGSPDTIRGMSVEMLFDFMSVRLDSAKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQPEANASFYISREDLHAVLTGQVKMV
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
     gene            21951..22226
                     /gene="ytcA"
                     /locus_tag="ACHKIR_000026"
     CDS             21951..22226
                     /gene="ytcA"
                     /locus_tag="ACHKIR_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtcA family lipoprotein"
                     /protein_id="HFO9987842.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPGWF
                     FCAIASLILTLITRRIIQRANINLAFVGIIYTALFALYAMLFWLAFF"
     gene            22245..23276
                     /gene="mdtN"
                     /locus_tag="ACHKIR_000027"
     CDS             22245..23276
                     /gene="mdtN"
                     /locus_tag="ACHKIR_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HFO9987843.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGIDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            23276..25327
                     /gene="mdtO"
                     /locus_tag="ACHKIR_000028"
     CDS             23276..25327
                     /gene="mdtO"
                     /locus_tag="ACHKIR_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HFO9987844.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQTVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAITQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAIAEGQCWQSDWRITESEAMAARECNLENICQTLLQLGQMDPNTPPTPA
                     TKPPSMVADAFTNPDYMRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQVQGAT
                     Q"
     gene            25324..26790
                     /gene="mdtP"
                     /locus_tag="ACHKIR_000029"
     CDS             25324..26790
                     /gene="mdtP"
                     /locus_tag="ACHKIR_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HFO9987845.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPFYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIVAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPQVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDSIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTLRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQASPVVEKK"
     gene            26988..29135
                     /gene="fdhF"
                     /locus_tag="ACHKIR_000030"
     CDS             26988..29135
                     /gene="fdhF"
                     /locus_tag="ACHKIR_000030"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:27405..27407,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HFO9987846.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNKTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDTDQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            29229..29918
                     /gene="yjcO"
                     /locus_tag="ACHKIR_000031"
     CDS             29229..29918
                     /gene="yjcO"
                     /locus_tag="ACHKIR_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YjcO"
                     /protein_id="HFO9987847.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(30336..31649)
                     /gene="gltP"
                     /locus_tag="ACHKIR_000032"
     CDS             complement(30336..31649)
                     /gene="gltP"
                     /locus_tag="ACHKIR_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HFO9987848.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(31991..32587)
                     /gene="nrfG"
                     /locus_tag="ACHKIR_000033"
     CDS             complement(31991..32587)
                     /gene="nrfG"
                     /locus_tag="ACHKIR_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HFO9987849.1"
                     /translation="MKQSQIPVKMLTTLTILMAFLCIGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            complement(32584..32967)
                     /gene="nrfF"
                     /locus_tag="ACHKIR_000034"
     CDS             complement(32584..32967)
                     /gene="nrfF"
                     /locus_tag="ACHKIR_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HFO9987850.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVAEGKNEAEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            complement(32960..34678)
                     /gene="nrfE"
                     /locus_tag="ACHKIR_000035"
     CDS             complement(32960..34678)
                     /gene="nrfE"
                     /locus_tag="ACHKIR_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HFO9987851.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDGACARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQARGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GRKRDE"
     gene            complement(34698..35654)
                     /gene="nrfD"
                     /locus_tag="ACHKIR_000036"
     CDS             complement(34698..35654)
                     /gene="nrfD"
                     /locus_tag="ACHKIR_000036"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HFO9987852.1"
                     /translation="MMQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTKPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRKNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPTVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            complement(35651..36322)
                     /gene="nrfC"
                     /locus_tag="ACHKIR_000037"
     CDS             complement(35651..36322)
                     /gene="nrfC"
                     /locus_tag="ACHKIR_000037"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HFO9987853.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            complement(36319..36885)
                     /gene="nrfB"
                     /locus_tag="ACHKIR_000038"
     CDS             complement(36319..36885)
                     /gene="nrfB"
                     /locus_tag="ACHKIR_000038"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HFO9987854.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            complement(36930..38366)
                     /gene="nrfA"
                     /locus_tag="ACHKIR_000039"
     CDS             complement(36930..38366)
                     /gene="nrfA"
                     /locus_tag="ACHKIR_000039"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HFO9987855.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            38759..40717
                     /gene="acs"
                     /locus_tag="ACHKIR_000040"
     CDS             38759..40717
                     /gene="acs"
                     /locus_tag="ACHKIR_000040"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HFO9987856.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINAPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSIEHVV
                     VLKRTGGKIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            41099..41413
                     /gene="yjcH"
                     /locus_tag="ACHKIR_000041"
     CDS             41099..41413
                     /gene="yjcH"
                     /locus_tag="ACHKIR_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HFO9987857.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            41410..43059
                     /gene="actP"
                     /locus_tag="ACHKIR_000042"
     CDS             41410..43059
                     /gene="actP"
                     /locus_tag="ACHKIR_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015123 - acetate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HFO9987858.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRNDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMLGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(43142..44791)
                     /gene="yjcE"
                     /locus_tag="ACHKIR_000043"
     CDS             complement(43142..44791)
                     /gene="yjcE"
                     /locus_tag="ACHKIR_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HFO9987859.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(44942..46291)
                     /gene="ghxP"
                     /locus_tag="ACHKIR_000044"
                     /gene_synonym="yjcD"
     CDS             complement(44942..46291)
                     /gene="ghxP"
                     /locus_tag="ACHKIR_000044"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HFO9987860.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFTTFPVMMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(46837..47301)
                     /gene="soxR"
                     /locus_tag="ACHKIR_000045"
     CDS             complement(46837..47301)
                     /gene="soxR"
                     /locus_tag="ACHKIR_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HFO9987861.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLISSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIREAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            47387..47710
                     /gene="soxS"
                     /locus_tag="ACHKIR_000046"
     CDS             47387..47710
                     /gene="soxS"
                     /locus_tag="ACHKIR_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HFO9987862.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(47713..49299)
                     /gene="pdeC"
                     /locus_tag="ACHKIR_000047"
     CDS             complement(47713..49299)
                     /gene="pdeC"
                     /locus_tag="ACHKIR_000047"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HFO9987863.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNSQLQT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIASVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSNEASAATFSPLIHLNDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLITHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWPGEQGQVMNPAEFIPLAEKEGMIEQI
                     TDYVIDNVFRDLGVYLATHVDRYVSINLSASDFHTSRLIARTNQKTEQYAVRPQQIKF
                     EVTEHAFLDVDKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMP
                     PQVFMQWMEQLPARELTRGQ"
     gene            49729..50010
                     /gene="yjcB"
                     /locus_tag="ACHKIR_000048"
     CDS             49729..50010
                     /gene="yjcB"
                     /locus_tag="ACHKIR_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HFO9987864.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
     gene            complement(50110..50646)
                     /gene="ssb1"
                     /locus_tag="ACHKIR_000049"
     CDS             complement(50110..50646)
                     /gene="ssb1"
                     /locus_tag="ACHKIR_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HFO9987865.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            50901..53723
                     /gene="uvrA"
                     /locus_tag="ACHKIR_000050"
     CDS             50901..53723
                     /gene="uvrA"
                     /locus_tag="ACHKIR_000050"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFO9987866.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLYARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(53758..54114)
                     /gene="yjbR"
                     /locus_tag="ACHKIR_000051"
     CDS             complement(53758..54114)
                     /gene="yjbR"
                     /locus_tag="ACHKIR_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFO9987867.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(54118..54534)
                     /gene="yjbQ"
                     /locus_tag="ACHKIR_000052"
     CDS             complement(54118..54534)
                     /gene="yjbQ"
                     /locus_tag="ACHKIR_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HFO9987868.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRAVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(54645..55358)
                     /gene="aphA"
                     /locus_tag="ACHKIR_000053"
     CDS             complement(54645..55358)
                     /gene="aphA"
                     /locus_tag="ACHKIR_000053"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HFO9987869.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            55759..55981
                     /locus_tag="ACHKIR_000054"
                     /pseudo
     CDS             55759..55981
                     /locus_tag="ACHKIR_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165333.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(56484..57677)
                     /gene="tyrB"
                     /locus_tag="ACHKIR_000055"
     CDS             complement(56484..57677)
                     /gene="tyrB"
                     /locus_tag="ACHKIR_000055"
                     /EC_number="2.6.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HFO9987870.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVADAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNGVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDSVIEILKARELIPFLDIAYQGF
                     GAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEI
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(57930..59009)
                     /gene="alr"
                     /locus_tag="ACHKIR_000056"
     CDS             complement(57930..59009)
                     /gene="alr"
                     /locus_tag="ACHKIR_000056"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFO9987871.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(59062..60477)
                     /gene="dnaB"
                     /locus_tag="ACHKIR_000057"
     CDS             complement(59062..60477)
                     /gene="dnaB"
                     /locus_tag="ACHKIR_000057"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFO9987872.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            60560..61543
                     /gene="qorA"
                     /locus_tag="ACHKIR_000058"
     CDS             60560..61543
                     /gene="qorA"
                     /locus_tag="ACHKIR_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HFO9987873.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(61709..61951)
                     /gene="pspG"
                     /locus_tag="ACHKIR_000059"
     CDS             complement(61709..61951)
                     /gene="pspG"
                     /locus_tag="ACHKIR_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HFO9987874.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(62085..63080)
                     /gene="dusA"
                     /locus_tag="ACHKIR_000060"
     CDS             complement(62085..63080)
                     /gene="dusA"
                     /locus_tag="ACHKIR_000060"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HFO9987875.1"
                     /translation="MPAESHTVYPAYRFSIAPMLDWTDRHCRYFLRLLSRNTLLYTEM
                     VTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEARGYDEINLNVGCPS
                     DRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDDQDSYEFLCDFINTV
                     SGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDFPHLTMSINGGIKSL
                     EEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDVDPVAVVRAMYPYIEREL
                     SQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVLEHALKLVADKR"
     gene            63236..63751
                     /gene="zur"
                     /locus_tag="ACHKIR_000061"
     CDS             63236..63751
                     /gene="zur"
                     /locus_tag="ACHKIR_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HFO9987876.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALEFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCSACVEVEACRHPEQCHH
                     DHSIQVKKKPR"
     gene            complement(63793..64002)
                     /gene="yjbJ"
                     /locus_tag="ACHKIR_000062"
     CDS             complement(63793..64002)
                     /gene="yjbJ"
                     /locus_tag="ACHKIR_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFO9987877.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVDSWEKRHDYRW"
     gene            complement(64118..65443)
                     /gene="dinF"
                     /locus_tag="ACHKIR_000063"
     CDS             complement(64118..65443)
                     /gene="dinF"
                     /locus_tag="ACHKIR_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HFO9987878.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRHLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            complement(65516..66124)
                     /gene="lexA"
                     /locus_tag="ACHKIR_000064"
     CDS             complement(65516..66124)
                     /gene="lexA"
                     /locus_tag="ACHKIR_000064"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFO9987879.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            complement(66234..66602)
                     /gene="dgkA"
                     /locus_tag="ACHKIR_000065"
     CDS             complement(66234..66602)
                     /gene="dgkA"
                     /locus_tag="ACHKIR_000065"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFO9987880.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            66773..69196
                     /gene="plsB"
                     /locus_tag="ACHKIR_000066"
     CDS             66773..69196
                     /gene="plsB"
                     /locus_tag="ACHKIR_000066"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HFO9987881.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPGVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            complement(69351..70223)
                     /gene="ubiA"
                     /locus_tag="ACHKIR_000067"
     CDS             complement(69351..70223)
                     /gene="ubiA"
                     /locus_tag="ACHKIR_000067"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HFO9987882.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSILVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(70236..70733)
                     /gene="ubiC"
                     /locus_tag="ACHKIR_000068"
     CDS             complement(70236..70733)
                     /gene="ubiC"
                     /locus_tag="ACHKIR_000068"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HFO9987883.1"
                     /translation="MSHPALTQLRALRYFTEIPALEPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            complement(70912..71832)
                     /gene="malM"
                     /locus_tag="ACHKIR_000069"
     CDS             complement(70912..71832)
                     /gene="malM"
                     /locus_tag="ACHKIR_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HFO9987884.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGIRGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTTDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPAPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            complement(72070..73410)
                     /gene="lamB"
                     /locus_tag="ACHKIR_000070"
     CDS             complement(72070..73410)
                     /gene="lamB"
                     /locus_tag="ACHKIR_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HFO9987885.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYTGNANTNTNFGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            complement(73482..74597)
                     /gene="malK"
                     /locus_tag="ACHKIR_000071"
     CDS             complement(73482..74597)
                     /gene="malK"
                     /locus_tag="ACHKIR_000071"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HFO9987886.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            74962..76152
                     /gene="malE"
                     /locus_tag="ACHKIR_000072"
     CDS             74962..76152
                     /gene="malE"
                     /locus_tag="ACHKIR_000072"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HFO9987887.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYQ
                     DELAKDPRIAATMDNAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            76306..77850
                     /gene="malF"
                     /locus_tag="ACHKIR_000073"
     CDS             76306..77850
                     /gene="malF"
                     /locus_tag="ACHKIR_000073"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HFO9987888.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            77865..78755
                     /gene="malG"
                     /locus_tag="ACHKIR_000074"
     CDS             77865..78755
                     /gene="malG"
                     /locus_tag="ACHKIR_000074"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HFO9987889.1"
                     /translation="MAMVQPKSQKTRLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRVTPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(78849..79259)
                     /gene="psiE"
                     /locus_tag="ACHKIR_000075"
     CDS             complement(78849..79259)
                     /gene="psiE"
                     /locus_tag="ACHKIR_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HFO9987890.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            79473..79751
                     /locus_tag="ACHKIR_000076"
     CDS             79473..79751
                     /locus_tag="ACHKIR_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9987891.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            complement(79798..81894)
                     /gene="yjbH"
                     /locus_tag="ACHKIR_000077"
     CDS             complement(79798..81894)
                     /gene="yjbH"
                     /locus_tag="ACHKIR_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFO9987892.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYNDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQTKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETKLAQKRVEPVVPQSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(81894..82631)
                     /gene="yjbG"
                     /locus_tag="ACHKIR_000078"
     CDS             complement(81894..82631)
                     /gene="yjbG"
                     /locus_tag="ACHKIR_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFO9987893.1"
                     /translation="MIKQTIVALILSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLARLAELAADSSADDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISRPGKQP
                     FTPGRDVASYLSGQNLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            complement(82628..83266)
                     /gene="yjbF"
                     /locus_tag="ACHKIR_000079"
     CDS             complement(82628..83266)
                     /gene="yjbF"
                     /locus_tag="ACHKIR_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HFO9987894.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWIEYQQVRYATARSVFKWDGTDTVKVGSDET
                     PVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            complement(83453..83695)
                     /gene="yjbE"
                     /locus_tag="ACHKIR_000080"
     CDS             complement(83453..83695)
                     /gene="yjbE"
                     /locus_tag="ACHKIR_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide production protein YjbE"
                     /protein_id="HFO9987895.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAGSAATSVSAGSSSA
                     TSVSTVSSAVGVALAASGGGDGSNTGTTTTTTTSTQ"
     gene            complement(84193..85842)
                     /gene="pgi"
                     /locus_tag="ACHKIR_000081"
     CDS             complement(84193..85842)
                     /gene="pgi"
                     /locus_tag="ACHKIR_000081"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFO9987896.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGKVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     regulatory      86079..86275
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            86367..87716
                     /gene="lysC"
                     /locus_tag="ACHKIR_000082"
     CDS             86367..87716
                     /gene="lysC"
                     /locus_tag="ACHKIR_000082"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HFO9987897.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHFNLFE"
     gene            87969..88241
                     /gene="yjbD"
                     /locus_tag="ACHKIR_000083"
     CDS             87969..88241
                     /gene="yjbD"
                     /locus_tag="ACHKIR_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HFO9987898.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(88242..89114)
                     /gene="rluF"
                     /locus_tag="ACHKIR_000084"
     CDS             complement(88242..89114)
                     /gene="rluF"
                     /locus_tag="ACHKIR_000084"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HFO9987899.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDVVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTVGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            89462..90409
                     /locus_tag="ACHKIR_000085"
     CDS             89462..90409
                     /locus_tag="ACHKIR_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HFO9987900.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGIVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVNALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDVALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIIGALRGKYINCLVTNSSTAELLLK"
     gene            90479..91285
                     /locus_tag="ACHKIR_000086"
     CDS             90479..91285
                     /locus_tag="ACHKIR_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO9987901.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVDELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSAKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTRLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVTDFVCYLLSQRASYITGVTTNIAGGKTRG"
     gene            91295..91702
                     /locus_tag="ACHKIR_000087"
     CDS             91295..91702
                     /locus_tag="ACHKIR_000087"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIA"
                     /protein_id="HFO9987902.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGNANVEAVEFVPGENAGDIV
                     AKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     HSMNVDELCEHLTQIAKQTCVVWKQLATAEEDF"
     gene            91702..92196
                     /locus_tag="ACHKIR_000088"
     CDS             91702..92196
                     /locus_tag="ACHKIR_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFO9987903.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQHIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIDTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            92262..93059
                     /locus_tag="ACHKIR_000089"
     CDS             92262..93059
                     /locus_tag="ACHKIR_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFO9987904.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIMLGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLSAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALLYIQLNPQWRKAEPQLQATASTALDQLDD"
     gene            93070..93894
                     /locus_tag="ACHKIR_000090"
     CDS             93070..93894
                     /locus_tag="ACHKIR_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFO9987905.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRRHLVFFNTTPAVCGPVIGVTAAMEEARANGAEIDDGTINGIKVG
                     LMGPLAGVGDPLVWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQL
                     GFRKGVNIVSDMGGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLLVSQTHAADGST
                     VTMTVQNILDQLCPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            93946..95175
                     /gene="sorE"
                     /locus_tag="ACHKIR_000091"
     CDS             93946..95175
                     /gene="sorE"
                     /locus_tag="ACHKIR_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HFO9987906.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVATNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVGGEATHVVIPNEVMEQDCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNAADAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAIGGGKKLVYTGKYLPLMSLTQIQDQALAAILARHQGIWS
                     GEAEQYLLAHAEAISHD"
     gene            95557..96246
                     /gene="pepE"
                     /locus_tag="ACHKIR_000092"
     CDS             95557..96246
                     /gene="pepE"
                     /locus_tag="ACHKIR_000092"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HFO9987907.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(96336..97967)
                     /gene="yjbB"
                     /locus_tag="ACHKIR_000093"
     CDS             complement(96336..97967)
                     /gene="yjbB"
                     /locus_tag="ACHKIR_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HFO9987908.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLPLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(98187..101870)
                     /gene="metH"
                     /locus_tag="ACHKIR_000094"
     CDS             complement(98187..101870)
                     /gene="metH"
                     /locus_tag="ACHKIR_000094"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFO9987909.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMEFLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDGLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGEDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPAEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPTELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANTQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLSGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            102070..102894
                     /gene="iclR"
                     /locus_tag="ACHKIR_000095"
     CDS             102070..102894
                     /gene="iclR"
                     /locus_tag="ACHKIR_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HFO9987910.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSNHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGIR"
     gene            complement(102911..104635)
                     /gene="aceK"
                     /locus_tag="ACHKIR_000096"
     CDS             complement(102911..104635)
                     /gene="aceK"
                     /locus_tag="ACHKIR_000096"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HFO9987911.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLRWQNKSRDIHYIVRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGNLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMAL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRK
                     QRFSVRFV"
     gene            complement(104851..106155)
                     /gene="aceA"
                     /locus_tag="ACHKIR_000097"
     CDS             complement(104851..106155)
                     /gene="aceA"
                     /locus_tag="ACHKIR_000097"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HFO9987912.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSAGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAVKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(106185..107786)
                     /gene="aceB"
                     /locus_tag="ACHKIR_000098"
     CDS             complement(106185..107786)
                     /gene="aceB"
                     /locus_tag="ACHKIR_000098"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HFO9987913.1"
                     /translation="MTEQATTTDELAFTRPYGEQEKHILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASIRDADWKIRGIPADLEDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEERNNQVLNKVKADKSLEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     MREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
     gene            complement(108055..108984)
                     /gene="metA"
                     /locus_tag="ACHKIR_000099"
     CDS             complement(108055..108984)
                     /gene="metA"
                     /locus_tag="ACHKIR_000099"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HFO9987914.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEFFRDVEAGLDPDVPYNYFPHNDPQNTPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            109141..109584
                     /gene="yjaB"
                     /locus_tag="ACHKIR_000100"
     CDS             109141..109584
                     /gene="yjaB"
                     /locus_tag="ACHKIR_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFO9987915.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAAYRAELEELVR
                     SFLPEAPLWVAVNERDRPVGFMLLSGQHMDALFIDPDVRGCGVGRMLVEHALSMAPEL
                     TTNVNEQNKQAVGFYKKMGFKVTGRSEVDDLGKPYPLLNLAYVGE"
BASE COUNT        26527 a        29461 c        28452 g        25256 t
ORIGIN      
        1 cagaaagata aaaacatcat gtgaattaaa aaaagaacga gtagagcatt aaccttatct
       61 taaataataa atggaggcaa aaagattatt ttctttttgc gtttcctttc aaatgaaaac
      121 gatcgtcgtc taaaatcagc agtacccccg acaaactcag ggattttgtg tataattgcg
      181 gcctttttcg gcaatctgcc gttttttggc gcttttgccc tgctgacttt tgaggaaatc
      241 cacatgtcat taccacactg cccaaaatgc aactccgaat acacctacga agataacggc
      301 atgtacatct gcccggaatg tgcctacgaa tggaacgacg cagaacctgc acaggaaagc
      361 gacgagctga tcgttaaaga tgctaacggc aatctgctgg ctgacggcga cagcgttacc
      421 atcattaaag atctgaaagt gaaaggtagc tcttcgatgc tgaaaattgg caccaaagtg
      481 aaaaacatcc gcctggttga aggcgaccat aacatcgatt gcaaaatcga cggttttggt
      541 ccgatgaaac tgaagtctga gtttgtgaaa aagaactgat tgtattgtga tcggtaggcc
      601 ggataaggcg tttacgccgc atccggcaac ggtggcgact gcctgatgcg acgctgaccg
      661 cgtcttatca ggcttccctc tcatctgtat aaatttcgaa ctacacttaa cgggcttctc
      721 ttaactgagg tcaccatcat gccgttaagt ccctacctct cttttgccgg taactgttcc
      781 gacgcgattg cctattatca acgtacgttg ggcgcggaac tgctctataa aatcagcttc
      841 ggcgaaatgc cgaaatcagc gcaggacagc gccgagaact gcccttccgg aatgcaattt
      901 cccgataccg ccatcgctca tgccaacgtg cgcattgcag gaagcgacat catgatgagc
      961 gatgctatac cgtcaggaaa agccagctac tccggcttta cgctagtgct cgattcgcaa
     1021 caggtcgaag aaggaaaacg ctggtttgat aaccttgcag ctaacggaaa aatcgaaatg
     1081 gcctggcagg aaactttctg ggcgcatggc ttcggcaaag tcaccgataa atttggcgta
     1141 ccgtggatga ttaatgtcgt caaacaacaa ccaacgcact aacccgccgg gagggccgcc
     1201 ctcccgcact gtcatcgaaa tctcgttaac tcttcatccg ttagtcactt ttagttaaca
     1261 aaatcgtcac aataatccgc cacgatggag ccactttttt agggaggctg catcatgcaa
     1321 acgattatcc gtgtcgagaa gctcgccaaa accttcaatc agcatcaggc gctgcacgcg
     1381 gttgatctga acattcatca cggtgaaatg gtggctctgc ttgggccgtc gggttccggc
     1441 aaatccaccc ttttacgtca cttaagcggt ttgattaccg gcgataaatc tgccggtagc
     1501 catatcgagc tgctgggccg cacagtccag cgcgaaggcc gcctggcccg cgatatccgc
     1561 aaaagccgcg ccaataccgg ctacatcttc caacaattca acctggtgaa tcgcctgagc
     1621 gtactggaga acgtgctgat tggcgcgctc ggcagcacgc cattctggcg cacctgtttt
     1681 agctacttca cccgcgagca gaaacagcgc gcgttacagg cgctgacccg cgttggcatg
     1741 gtgcactttg cccatcagcg cgtttccacc ctctccggcg gacagcaaca gcgtgtggcg
     1801 attgcccgcg cgctgatgca gcaggcgaag gtgattctgg cggacgaacc catcgcctcg
     1861 ctggacccgg aatcggcgcg catcgtgatg gacaccctgc gcgacattaa ccagaacgac
     1921 ggcattaccg tggtcgtcac gctgcatcag gtggattacg ccctgcgcta ctgcgaacgc
     1981 atcgtcgccc tgcgccaggg gcacgtcttc tacgacggca gcagccaaca gtttgataac
     2041 gaacgttttg accatctcta ccgcagcatc aaccgcgtcg aagaaaacgc gaaagctgcc
     2101 tgacatcccc atcattgagg aaaacgaatg aacgctaaga taattgcctc gctgaccttc
     2161 accagcatgt tcagcctcag caccctgtta agcccggcgc acgccgaaga gcaggaaaag
     2221 gcgctgaatt tcggcattat ttcaacggaa tcacagcaaa acctgaaacc gcaatggacg
     2281 ccgttcttac aggatatgga gaagaagctg ggcgtgaagg tcaacgcctt ctttgccccg
     2341 gactacgcgg gcattatcca gggaatgcgc ttcaacaaag tggatatcgc ctggtacggc
     2401 aatctgtcag cgatggaagc ggtggatcgc gccaatggcc aggtcttcgc ccagacggtc
     2461 gcggcggatg gatcgccggg ttactggagc gtgttgatcg tcaacaaaga tagtccgatc
     2521 aacaacctga acgatctgct ggccaagcgt aaagatctca ccttcggcaa tggcgatcct
     2581 aactccacct caggcttcct cgtccccggc tactacgtct tcgccaaaaa caatatctcc
     2641 gccagcgact tcaagcgcac cgtcaacgcc gggcatgaaa ccaacgcgct ggccgttgcc
     2701 aacaagcagg tggatgttgc caccaacaac accgaaaacc tcgacaagct gaaaacctcc
     2761 gcaccggaga agctcaaaga actgaaggtg atctggaaat cgccgctgat cccaggcgat
     2821 ccgatcgtct ggcgcaaaaa tctgtccgaa accaccaaag acaagatcta cgacttcttt
     2881 atgaactacg gcaaaacgcc ggaagagaaa gcggtgctgg aacgcctggg ctgggccccg
     2941 ttccgcgcct ccagcgacct gcaactggtg ccgattcgcc agctcgcgct gtttaaagag
     3001 atgcagggcg tgaaaggcaa taaagggctg aatgagcagg acaagctggc aaaaaccagc
     3061 gccattcagg cgcaactgga tgacctggac cgcctgaaca acgcgttaag cgcgatgagt
     3121 tcggtgagta aagcggtgca gtaaatcgta ggtcggataa gacgccccgg cgtcgcatcc
     3181 gacaatgtgc agacgttgat gccggatgcg gtgcaagcac cttatccggc ctacagaccg
     3241 gagccaaaca tgcaaaccat caccatcgcc ccacccaagc gcagctggtt ctcgcttctg
     3301 agctgggcca ttgttctcgc cgtgttggtc gtctcgtggc agggcgcgga aatggccccg
     3361 cttacgctga tcaaagacgg cggcaacatg gcaaccttcg ccgccgactt cttcccgccc
     3421 gatttcagcc agtggcagga ttacctcacc gaaatggccg tcacgctgca aatcgccgtc
     3481 tggggcaccg cgctggcggt ggttctctcc atccccttcg gtctgatgag cgccgaaaac
     3541 ctggtgccgt ggtgggttta ccagcccgta cgccgcctga tggacgcctg ccgcgccatt
     3601 aacgaaatgg tcttcgccat gctgttcgtg gtcgccgtcg gccttggccc gttcgctggc
     3661 gtgctggcgc tgtttatcca caccaccggc gtgctctcca agctgctttc cgaagcggta
     3721 gaagcgatcg aacccggccc ggtggagggc attcgcgcca ccggtgccaa caagctggaa
     3781 gagatcctct acggcgtgct gccacaggtg atgccgctgc tgatctccta ctccctctat
     3841 cgcttcgaat ccaacgtccg ctcggcgacc gtcgtcggca tggtcggtgc aggcgggatc
     3901 ggcgtcaccc tgtgggaagc gattcgcggt ttccagttcc agcaaacctg cgccctgatg
     3961 gtgctcatca tcgtcacggt cagcctgctg gatttcctct ctcaacggtt gcgtaagcac
     4021 tttatctgat aagcgaggca ttgatagcta tgcacttgtc tacacatccg accagctacc
     4081 caacacgcta tcaggagata gccgcaaaac ttgagcagga gcttcgtcaa cactaccgct
     4141 gcggcgacta tcttcccgcc gagcagcaac tggcagcgcg ctttgaggtg aaccgccaca
     4201 ccctgcgccg cgccatcgac caactggtgg aaaaaggttg ggtacagcgc cgtcagggcg
     4261 tcggcgtgct ggtgctgatg cgcccgttcg attacccgct caacgcccag gcgcgtttta
     4321 gccagaacct gctggaccaa ggcagccatc ccaccagcga aaaattgcta tcggtgctgc
     4381 gccccgcctc cggtcacgtc gctgacgcgc tggggataac cgagggggag aacgtcatcc
     4441 acctgcgcac cctgcgccgg gtcaacggcg tcgcgctctg tttaatcgac cactactttg
     4501 cggacctcac cctctggccg acgctgcaac atttcgacag cggttcgctg cacgattttc
     4561 tgcgcgagca aacggggatt acgctacgcc gcagtcagac gcggatcagc gcccgccgcg
     4621 cccaggccaa agagtgccag cgtcttgaaa tcccgaatct atcgccgctg ctgtgcgtgc
     4681 gcacccttaa ccaccgtgac ggtgaaagca gcccggcgga gtactccgtc agcctgacgc
     4741 gcgccgacat gattgaattc actatggagc actgaatgca cgcagatacc gcgacccgcc
     4801 agcactggat gtccgtactg gcgcacagcc aaccgactga gctggcagcc cgcctgaacg
     4861 cgctgaacat caccgccgac tatgaagtaa tccgcgccgc tgaaaccggc ctggtgcaga
     4921 ttcaggcgcg gatgggtggt accggcgaac gcttttttgc tggcgacgcc acgctcaccc
     4981 gcgccgccgt acgcctgact gacggcacgc tcggctacag ctgggtgctg gggcgcgaca
     5041 aacagcacgc cgaacgctgc gcgctgattg acgcgctgat gcagcaaacc cgccattttc
     5101 aaaacttatc agaaactctt attgccccgc tgaacgctga ccgtatggca cgcattgccg
     5161 cacgccaggc cgaagtaaac gccagccggg tcgacttctt tacgatggtt cgcggagata
     5221 acgcatgacc ctggaaaccg cttttatgct tcccgtgcag gatgcccagc acagttttcg
     5281 tcgcctgtta aaagccatga gcgagccggg cgtgattgtc gccctgcatc agctcaaacg
     5341 cggctggcag ccgctgaata tcgccaccac cagcgtgctg ctgacgctgg ccgataacga
     5401 cacgccggtg tggctttctg cgccattaag taacgatatc gtcagccaga gcctgcgttt
     5461 tcataccaac gcgccgctgg tcaatcagcc ggaactggcg accttcgcgg taacggatga
     5521 ggcgatttcc agcgaacagc ttaacgccct ttccagcggc accgctgttg cgccagaagc
     5581 gggcgcgacg ctgattttac aggtcgtcag cctgagcggc gggcgcatgc tgcgccttac
     5641 tggcgcgggt attgccgaag aacgaatgat cgccccgcag ctgccggagt gcattctgca
     5701 cgaacttacc gagcgcccgc acccattccc gctcggcatc gacctgatcc tgacctgcgg
     5761 cgagcgcctg ctggctatcc cgcgaaccac tcatgtggag gtatgctgat gtacgttgcc
     5821 gtgaaagggg gcgagaaggc gatcgacgcc gcccacgccc tgcaagagag ccgacgccgg
     5881 ggcaataccg atttgcctga actgagcgtc gcccagattg aacagcagct taatcttgca
     5941 gtagatcgcg tgatgaccga aggcggcatt gccgaccgcg aactggcggc gctggcgctg
     6001 aaacaggcca gcggcgataa cgtcgaagcg attttcctgc tacgcgccta ccgcaccacg
     6061 ctggcgaagc tcgcagtaag cgagccgctc gacaccacgg agatgcgtct cgaacggcgt
     6121 atttcagcgg tttataaaga tattcccggc ggccagctgc ttggcccaac ctacgactac
     6181 acccatcgcc tgctcgattt caccctgctg gcaaacggcg aagcgccgac gctgaccaca
     6241 gccgacagcg aacaacagcc gtcgccgcac gttttcagcc tgctggcgcg tcaggggctg
     6301 gcgaagtttg aagaagatag cggcgcacag ccggacgaca tcacccgcac gccgccggtt
     6361 tacccctgct cacgctcctc ccgcctgcaa caattaatgc gcggcgacga aggctatttg
     6421 ctggcgctgg cctactccac ccagcgtggt tacggacgca atcatccgtt cgcaggcgaa
     6481 atccgcagcg gctatatcga cgtgtcgatt gtgcaggaag agctgggatt tgcggtgaac
     6541 gtcggcgaac tgctgatgac cgaatgtgaa atggtcaacg gttttatcga cccgccggat
     6601 gagccgccgc acttcacgcg cggctacggg ctggtgttcg gcatgagcga gcgcaaagcg
     6661 atggcgatgg cgctggttga ccgcgctcta caagccccgg agtacggcga gcacgcgaca
     6721 ggcccggcgc aggatgaaga gttcgtgctg gcgcatgccg acaacgtcga agccgcaggc
     6781 tttgtctcgc accttaaact cccgcactac gtcgatttcc aggccgaact ggagctactc
     6841 aaacgtctgc aacaggagca gaaccatggc taatctgagc ggctacaact ttgcctacct
     6901 cgacgagcag accaaacgca tgatccgccg cgccatctta aaagcggtgg cgatccccgg
     6961 ttatcaggtg ccgtttggcg ggcgcgagat gccgatgccg tacggctggg gaaccggcgg
     7021 catacagctt accgccagcg tgattggcga aagcgatgtg ctgaaggtga ttgaccaggg
     7081 cgcggacgac accaccaacg cggtgtcgat ccgtaacttc tttaagcgcg tgaccggggt
     7141 aaacaccact gaacgtacgg acgatgcgac gcttatccag acgcgccacc gcatccccga
     7201 aacgccgctg accgaagatc agatcattat tttccaggtg ccgatccccg aaccgctgcg
     7261 ctttatcgag ccgcgcgaaa cggaaacccg caccatgcac gcgctggagg agtacggcgt
     7321 gatgcaggtg aagctgtatg aagatatcgc ccgcttcggt catatcgcca ccacctacgc
     7381 ctatccggtg aaggtaaacg ggcgctacgt aatggacccg tcgccgatcc cgaaatttga
     7441 taacccgaaa atgaacatga tgcccgccct gcaactgttc ggcgcggggc gcgagaagcg
     7501 catctatgcg gtgccgccgt ttacccgcgt ggaaagcctc gatttcgacg atcacccgtt
     7561 caccgttcag cagtggaatg agccatgcgc catctgcgga tcgacccaca gctatcttga
     7621 tgaagtggtg ctggatgacg ccggaaaccg catgtttgtc tgctccgata ccgattattg
     7681 ccgccaacag agcgaggcga aaagccaatg aatcaaccgt tactttcggt caataacctg
     7741 acccaccttt acgcgccggg caaaggcttt agcgatgtct cttttgattt atggccgggg
     7801 gaagtgctgg gcatcgtcgg ggaatccggc tccgggaaga ccacgctgct gaagtcgatc
     7861 tccgcgcgcc tgacgccgca gcaaggggaa attcgctatg aaaaccgttc cctctatggc
     7921 atgagcgagg ccgaccgccg tcgcctgctg cgcaccgaat ggggcgtggt gcatcagcat
     7981 ccactcgacg gcctgcgccg ccaggtgtcg gcaggcggca atatcggcga gcggctgatg
     8041 gcgaccgggg cgcgtcatta cggcgatatt cgtgccaccg cgcagaagtg gctggaagag
     8101 gtggaaattc ccgccaaccg gatcgacgac ctgccgacca ccttttccgg cggtatgcag
     8161 cagcgtttgc agattgcccg caacctggtg acgcatccga agctggtatt tatggacgag
     8221 ccaaccggcg ggctggatgt atctgtgcag gcgcgcctgc tcgacctgct gcgcggcctg
     8281 gtggtggagc tgaacctggc ggtggtgatt gtcacccacg atttaggcgt cgcccgcctg
     8341 ctggcggacc gtttgctggt gatgaagcag gggcaagtgg tggagagtgg gttaaccgac
     8401 cgcgtgctcg acgacccgca ccatccgtat acacaactgc tggtgtcatc ggttttgcag
     8461 aattgagccg gtgccggatg cggcgtaaac gccttatccg gcctacaaat gcgctccccg
     8521 taggtcggat aagacgcgtc agcgtcgcat ccgacacccg aaccacgagg cgaaaaatga
     8581 ttaacgtaca aaacgtcagt aaaaccttca tcctgcatca gcaaaacggc gtgcgcctgc
     8641 ccgtcttaca acgtgcctcg ctcaccgtca acgcaggcga atgcgtggtg cttcacggcc
     8701 actccggcag cggaaaatca accctgctgc gctcgctgta cgccaactat ctgcccgacg
     8761 aagggcaaat ccagatcaaa cacggtgagg aatgggtaga cctggtcacc gcgccagccc
     8821 gcaaagtggt ggagatccgc aaaaccacca tcggctgggt gagccagttt ctgcgcgtca
     8881 tcccgcgtat ttcggcactg gaagtggtga tgcagcctct gctcgatacc ggcgtaccgc
     8941 gtgaagcctg cgccgctaaa gccgcccgat tactgacgcg cctgaacgtg cctgaacgcc
     9001 tgtggcacct ggcaccatcg accttctccg gcggcgaaca gcagcgcgtc aacatcgccc
     9061 gtggctttat cgtcgactat ccgattctgc tgctggatga acccaccgcc tcgctggacg
     9121 ctaaaaacag cgccgcggtg gtggaattaa ttcgcgaagc caaagcccgt ggcgcggcca
     9181 tcgtcggtat cttccatgac gaagccgtac gcaatgacgt cgccgaccgc ctgcacccaa
     9241 tgggagtctc ttcatgatta tcaataacgt caaactggtg ctggaaaacg aagtggtgca
     9301 cggttcactg gaagtacagg atggcaaaat tcgcgccttt gccgaaagcc agagccgcca
     9361 gccggaagcg atggacggcg aaggtggctg gctgctgccg gggctgattg agctgcatac
     9421 cgataatctg gataaattct tcaccccgcg tccaaaagtg gactggcccg cccactcggc
     9481 aatgagtagc cacgacgcgc tgatggtggc gagcggcatc accaccgtgc tggacgcggt
     9541 ggcgattggc gacgtgcgcg acggcggcga tcggctggag aatctggaga agatgatcaa
     9601 cgccatcgaa gagacgcaga aacgcggcgt caaccgcgcc gagcaccgtc tgcatctgcg
     9661 ctgcgaactg ccgcatcaca ccacgctgcc gctgtttgaa aaactggtgc agcgcgaacc
     9721 ggtgacgctg gtgtcgctga tggatcactc gccgggccag cgccagttcg ctaaccgcga
     9781 gaagtatcgc gaatattatc agggcaaata ctcgctcacc gaagcgcaga tgcagcagta
     9841 tgaagaagag cagctggcgc tcgccgcacg ctggtcgcag ccaaatcgcg aaaccatcgc
     9901 cgcaatgtgc cgcgcgcgac acattgcgct cgccagccac gatgacgcca cgcacgccca
     9961 cgttgctgaa tctcaccaac ttggcagcgt aatcgccgaa tttcccacca cgttcgaagc
    10021 ggcagaagcc tcgcgcaagc atggcatgaa cgtgctgatg ggcgcaccga atatcgtgcg
    10081 cggcggttcg cactccggca acgtggcggc cagtgaactg gcgcagcttg gcctgctgga
    10141 tatcctctct tccgactact accccgccag cctgctcgat gcggcatttc gcgtcgccga
    10201 tgacgagagc aaccgcttta cgctgccgca ggcggtgaag ctggtgacta aaaatccggc
    10261 gcaggcgctt aatcttcagg atcgcggggt gattggcgag ggcaaacgcg ccgacctggt
    10321 gctggcgcat cgcaagggca atcacattca tatcgaccac gtctggcgtc agggtaaaag
    10381 ggtgttctga tgacgggaaa actgatttgg ttaatggggg cctctggctc cggaaaagac
    10441 agtctgttga cggaactccg ccagcgggaa caaactcagc tactggtagc gcatcgctac
    10501 atcacgcgcg ccgccagcgc cggaagtgaa aaccatatcg ccctgagcga gcaggagttt
    10561 tttacccgcg cggggcagaa ccttctggcc ttaagctggc acgctaacgg tctgtattat
    10621 ggcgtcggcg tcgagattga cctctggctg cacgctggat ttgacgtggt ggtcaacggc
    10681 tcacgcgccc atctgccgca ggcgcgggcg cgctatcaat cggcgctgct gcccgtttgt
    10741 ttacaggttt cgccagagat cctgcgccag cgcctggaaa accgtggtcg tgaaaatgcc
    10801 agtgaaatta acgcccggct ggcgcgcgct gcccgctata ctcctcagga ttgtcttacg
    10861 ctcaataatg acggcagcct gcgccagtcg gtcgacaagt tgctgacgct gattcatcag
    10921 aaggagaaac accatgcctg cttgtgagct tcgcccggct acgcagtacg acaccgacgc
    10981 agtttacgcg ctgatttgtg agctaaaaca ggcggagttt gaccaccagg cgtttcgcgt
    11041 ggggtttaac gccaatctgc gcgatccgaa catgcgctac caactggcgc tgctggatgg
    11101 cgaagttgtc ggcatgatcg gcctgcattt gcagtttcat ctgcatcatg tcaactggat
    11161 cggcgaaatt caggaactgg tggtgatgcc gcaggcgcgc ggtctgaatg tcggcagcaa
    11221 attactggca tgggcagaag aagaagcccg ccaggccggg gccgaaatga ctgagctttc
    11281 caccaacgtg aagcgccacg acgcgcaccg attttacctg cgcgaaggct acgagcagag
    11341 ccacttccgc ttcaccaagg tgctgtaaca tgagcctgac cctcacactt actggcactg
    11401 gcggcgcaca gggcgttccc gcatggggct gtgagtgcgt ggcctgcgtc agagcgcggc
    11461 gctcaccgca gtatcgccgc cagccatgca gcggcgtcgt gaagtttaac gacgcgatca
    11521 ccctgatcga cgccgggcgg cacgatctcg ccgatcgctg gtcgcccgga tcgttccagc
    11581 agtttttgct gacgcactac catatggatc acgtgcaggg gctgtttccg ctgcgctggg
    11641 gcgtgggcga tcagatcccg gtctacggcc cgccggatga acagggctgc gacgatctat
    11701 tcaaacattc gggcctgctt gatttcagcc acacggtgga gccatttgtg atgtttgatt
    11761 tgcagggatt acaggtcacg ccgctaccgc tcaaccactc aaaactcact ttcggctatt
    11821 tactggaaac ggcgcacagc cgggtggcgt ggctatctga caccgcaggc ctgccggaaa
    11881 aaacgctgaa atttttacgc aataatcagc cgcaggtaat ggtgattgat tgcagccacc
    11941 cgccgcgcga ggatgcaccg cgtaatcact gtgatttaaa taccgtgctt gcgctgaatc
    12001 aggttatccg ctcgccgcgg gtgattctga cccatatcag ccatcagttt gatgcgtggc
    12061 tgatggaaaa cgcgctacca tcggggtttg aggcggggtt tgatgggatg gagattgagg
    12121 tggcgtgagg gaaaataccc gcgccggccc ctatatcaac gcccatactc atcttccatt
    12181 ctccgctctt catcttccag ttgccgacgc tcctgatcaa gctggcgctg gcgatcgtcc
    12241 agctgcctgc ggcgatcttc aaactggcgg cggcggtcat catattgtct gcgccgatcg
    12301 tcgctcactt cacgctgcca gccgtcgtcg cgcgaatctt catagtctcg cccacggtca
    12361 gggttataag cgtcgttaat cgcctgctga atattgccaa tggtgtcgtc gataatatcg
    12421 gcctgggccg gaacggtgga cagcgtgagc agggtgaata aaagaaatag cggaaagcgt
    12481 ttcattagcc aacctcaaaa agaaactcta tccacattag tcattactca tccatgcaag
    12541 tagtggacga atctcaattt ctccgctgct ctattgccgt aatcgcctcc acgcgttgtt
    12601 gatgacgacc gccttcgtac tgtgcgccca gccacgcatc gacaatcatt tttgccagtt
    12661 cgaggccaac cactcgtgaa ccaaaagcca gcacgttggt gtcgttatgc tgccgcgaaa
    12721 gttgcgcgga ataaggttcg ctacaaacga ccgcgcgaat tccggcaaac ttgttcgccg
    12781 ctatcgaaat accgacgcca gtgccacaaa tcaaaatccc gccatcagcc tctccgccag
    12841 caacggccag tgcgacgtga ctggcgtaat gcggataatc agtacgctct gacgaccagg
    12901 ttcctttatc aatcacttca acgccacgct caactaaatg tgccactatt tcatgtttta
    12961 aaatgaaacc gacatgatca cagccaaatg caatcttttt cattcatctt ccccgcccgc
    13021 gcaaaacttc acaatcaaaa aacatttgtg atgtaaatga attaaaaaca cccaaaatcg
    13081 atgaaatccg ccttcccgat ctgttttaca gagttacatc tggcagcgca atagcgttaa
    13141 agacactcac tatccagcaa catctatcat ctaataaacc aggaaaacaa ataacatcat
    13201 gtttttaaag tgattaaatg aaatgaaatt ttaagccact ctccattatt cacaacaaaa
    13261 caaactttat aaattttatt tttttgtgaa gttgccagca tcttttctgt tcttgctgtg
    13321 gtgatatagt ggcgtcttca attcaaggac aagagaacgt gatgagccag tcagagtttg
    13381 attcagcgct tccgaacggt atcgggttag cgccttacct gcgaatgaag caggaaggaa
    13441 tgacagaaaa cgaaagccgc atcgtggagt ggttactcaa acccggtaac ctgagttgtg
    13501 cacccgcaat taaagatgtc gcagaagctc tggcggtatc tgaagcgatg atcgttaagg
    13561 tatcaaagct gctggggttt agcggctttc gtaacttacg cagtgcgctg gaagattatt
    13621 tttctcagtc agaacaagta ttgccttccg agttggcttt tgatgaagcg ccgcaggatg
    13681 tggtgaataa ggtatttaac atcactttac gcaccattat ggaaggtcag tcgatcgtca
    13741 acgttgatga gatccaccgt gccgcccgct ttttctatca ggccagacag cgggatttgt
    13801 acggtgccgg aggatcaaat gcaatctgtg ctgatgtaca gcacaagttt ttgcgcattg
    13861 gcgtacgctg ccaggcctat ccggacgctc acatcatgat gatgtctgct tcgttgttac
    13921 aggaaggaga tgtggtgctg gtggtgaccc attccgggcg aaccagtgat gtaaaagcgg
    13981 ctgtagaact ggcaaaaaag aacggggcaa agattatttg tataacgcat agctaccatt
    14041 caccgatagc gaagctggcc gattatatta tttgctcacc ggccccggaa acaccgttat
    14101 taggtcgtaa tgcctcggca agaatattac aactaacttt gctggacgcg ttttttgtct
    14161 ctgtcgccca gctcaacatt gaacaagcta atattaatat gcaaaaaacc ggcgcaattg
    14221 ttgatttctt ctcaccaggc gcgctgaaat aagaaaaagg taattccgcc atcgcggagt
    14281 tacctatccc cttaccgtga gaacatgatt atgaataaat atctgaaata tttcagcggc
    14341 acactcgtgg gcttaatgtt gtcaaccagc gcttttgctg ccgccgaata tgccgtcgta
    14401 ttgaaaacac tctccaatcc attttgggta gatatgaaaa aaggcattga agatgaagca
    14461 aaaacgctgg gcgtcagcgt tgatattttt gcctctcctt cagaaggcga ttttcaatct
    14521 caattgcagt tatttgaaga tctcagtaat aaaaattaca aaggtatcgc cttcgcacca
    14581 ttatcctcag tgaacctggt catgcctgtc gcccgcgcat ggaaaaaagg catttatctg
    14641 gtcaatctcg atgaaaaaat cgacatggat aatctgaaaa aagcaggcgg taatgtggaa
    14701 ggttttgtca ccaccgataa tgttgccgtc ggggcgaaag gcgcgtcatt cattattgac
    14761 aagctgggtg ccgaaggggg tgaagtcgca atcattgagg gtaaagccgg taacgcctcc
    14821 ggtgaaacgc gtcgtaatgg tgccaccgaa gccttcaaaa aagcaagcca gatcaagctt
    14881 gtcgccagcc agcctgccga ctgggaccgt attaaagcac tggatgtcgc cactaacgtg
    14941 ttgcaacgta atccgaatat taaagcgatc tattgcgcga atgacacgat ggcaatgggt
    15001 gttgctcagg ctgtcgcaaa cgccggaaaa acgggaaaag tgctggtcgt cggtacagat
    15061 ggcattccgg aagcccgcaa aatggtggaa gccggacaaa tgaccgcgac ggttgcccag
    15121 aacccggcgg atatcggcgc aacgggtctg aagctgatgg ttgacgctga aaaatccggc
    15181 aaggttatcc cgctggataa agcaccggaa tttaaactgg tcgattcaat cctggtcact
    15241 caataaaact aaatgatgcc ggatggtgat gctaatgaag tgtcttatcc ggccaacaaa
    15301 ataacctgaa cgtagacctg ataagtgtag cccatcaggc aataatacga tatttcgtgc
    15361 agagaggtta tttatggcca cgccatatat atcgatggcg gggatcggca agtcctttgg
    15421 tccggttcac gcattaaagt cggttaattt aacggtttat cctggtgaaa tacatgcatt
    15481 actaggagaa aatggcgcgg gtaaatccac gctaatgaaa gttttatccg gaatacatga
    15541 gccgaccaaa ggcaccatta ccattaataa cattaactat aacaaactgg atcataaatt
    15601 agcggcacaa ctcggcatcg ggattattta tcaagaactc agcgttattg atgaattaac
    15661 cgtactggaa aatttatata ttggtcgtca tctgacgaaa aaaatctgtg gcgtcaatat
    15721 tatcgactgg cgagaaatgc gtgtccgcgc cgccatgatg ttattacgcg tgggcttgaa
    15781 agttgattta gatgagaaag tggcgaattt atctatcagc cacaagcaga tgctggaaat
    15841 tgccaaaacg ctgatgctcg acgccaaagt catcatcatg gatgaaccca cctcctcact
    15901 cactaataaa gaggtggact atttgtttct gatcatgaat cagttgcgta aggagggtac
    15961 ggccatcgtc tatatctcgc ataaattggc ggaaattcgc cgtatttgcg accgctatac
    16021 ggtgatgaaa gacggcagca gcgtttgcag cggcatggtg agcgatgtgt caaatgacga
    16081 tatcgtccgt ctgatggtag gtcgcgaact gcaaaaccgt tttaacgcga tgaaggagaa
    16141 tgtcagcaac cttgcgcacg atacggtttt tgaggtgcgc aacgtcacca gccgtgacag
    16201 aaaaaaggtc cgggatatct catttagcgt ctgccgggga gaaatattag gctttgccgg
    16261 actggtcggt tccggacgta ctgaactgat gaactgcctg tttggcgttg ataaacgcgc
    16321 tggcggagaa atccgtctta atggcaaaga tatctctcca cgttctcccc tggatgccgt
    16381 gaaaaaaggg atggcttaca tcactgaaag ccgccgggat aacggctttt tccccaactt
    16441 ttctatcgct cagaacatag ctatcagccg cagtctgaaa gacggcggct ataaaggcgc
    16501 gatggggttg tttcatgaag ttgacgagca acgtaccgct gaagcccagc gcgaactgct
    16561 ggcgctgaaa tgtcattcgg taaaccagaa catcactgaa ctctccggcg gtaaccagca
    16621 gaaagtcctg atctccaaat ggctgtgctg tcacccggaa gtgatcattt tcgatgaacc
    16681 tacccgtggc atcgacgttg gcgcgaaagc cgaaatttac aaagtgatgc gccaactggc
    16741 ggacgacgga aaagtcatcc tgatggtgtc atctgaacta cctgaaatta tcaccgtctg
    16801 cgaccgcatc gccgtgttct gcgaaggacg actgacgcaa atcctgacca atcgcgatga
    16861 catgagcgaa gaggagatta tggcatgggc tttaccacac gagtaaaaag cgaagcgagc
    16921 gagaagaaac cgttcaactt tgcgctgttc tgggataaat acggtacttt ttttatcctg
    16981 gcgatcatcg tcgccatttt tggttcgctg tcgccagaat attttctgac caccaataat
    17041 attacccaga tttttgtgca aagctccgtg acggtactga tcggcatggg ggaattcttc
    17101 gctatcctgg tcgccggtat cgacctttcg gttggcgcga ttctggcgct ttccggtatg
    17161 gtgaccgcta aactgatgtt ggcaggtatt gatccgtttc tcgcggcgct aattggcggt
    17221 gtactggttg gcggcgcact gggggcgatc aacggctgcc tggtcaactg gacgggacta
    17281 cacccgttca ttatcaccct tggcaccaac gctattttcc gtgggatcac gctggtgatc
    17341 tccgatgcca actcggtata cggcttctca tttgacttcg tgaacttctt tgccgccagc
    17401 gtaattggga tacctgtccc cgttatcttc tcgctaattg tcgcgctcat cctttggttt
    17461 ctgacaacgc gtatgcggct cgggcgcaac atctacgcac tgggcggcaa caaaaactcg
    17521 gcgttctatt ccgggattga cgtgaaattc cacatcctgg tggtgtttat catctccggt
    17581 gtttgtgcag gtctggcagg cgtcgtctca actgcacgac tcggtgccgc agaaccgctt
    17641 gccggtatgg gttttgaaac ctatgccatt gccagcgcca tcattggcgg caccagtttc
    17701 ttcggcggca aggggcgcat tttctctgtg gtgattggcg ggttgatcat cggcaccatc
    17761 aacaacggtc tgaatatttt gcaggtacaa acctattacc aactggtggt gatgggcgga
    17821 ttaattatcg cggctgtcgc ccttgaccgt cttatcagta agtaaggaat tgaacatgaa
    17881 aatctccccc tcgttaatgt gtatggatct gctgaaattt aaagaacaga tcgaatttat
    17941 cgacaaccat gccgattact ttcatatcga tatcatggac ggccactttg tgccgaatct
    18001 gacgctctca ccgttcttcg taagccaggt aaaaaaactg gcgagtaaac cgctcgactg
    18061 ccatctgatg gtaacgcgtc cgcaggatta cattgctcaa ctggcgcgtg cgggagctga
    18121 tttcatcact ctgcatccgg aaaccatcaa cggccaagcg ttccgcctga ttgatgaaat
    18181 ccgccgtcat ggcatgaaag tggggctgat tcttaatccg gaaacgccgg ttgaggccat
    18241 gaaatactat atccataagg ctgataaaat tacggtcatg actgtcgatc ccggttttgc
    18301 cggacaaccg ttcattcctg aaatgctgga taaacttgcc gaactgaagg catggcgtga
    18361 acgagaaggt ctggagtacg aaattgaggt ggacggttcc tgcaaccagg caacttacga
    18421 aaaactaatg gcggcagggg cggatgtctt tatcgtcggc acttccggcc tatttaatca
    18481 tgcggaaaat atcgacgaag catggagaat tatgaccgcg cagattctgg ctgcaaaaag
    18541 cgaggtacag cctcatgcaa aaacagcata acgtcgtagc gggcgtggat atgggggcaa
    18601 cgcatatccg cttttgtctg cggacagcag aaggtgaaac gctacactgc gaaaaaaagc
    18661 ggaccgcaga agttattgct cccgacctgg tgtcgggtat cggcgaaatg attgacgagc
    18721 aactcaggcg ctttaacgct cgctgtcatg gtctggtgat gggatttccg gcgctggtca
    18781 gtaaagataa acgcaccatt atttctacgc ctaacctgcc gttaacagcg gcagatttat
    18841 atgatctcgc cgataagctc gaaaatacgc tgaattgtcc ggttgagttt tcccgcgacg
    18901 ttaacctgca actctcctgg gacgtagtag aaaatcgcct tacgcaacaa ctggttctgg
    18961 cagcctatct cggtacgggg atggggttcg cagtgtggat gaacggtgcg ccgtggacgg
    19021 gtgcgcacgg tgtggcaggc gaactgggcc atatccccct gggagatatg acccaacact
    19081 gcgcgtgtgg caattctggg tgcctggaaa ccaattgctc tggaatggcg ctaagacgct
    19141 ggtacgaaca acagccccga aattacccat tgagcgatct tttcgtccat gcggaaaacg
    19201 cccctttcgt ccagagtctg cttgaaaacg cggcacgagc cattgccacc agcattaatc
    19261 tgttcgatcc cgatgcggtg attctgggtg gtggcgtgat ggatatgccc gccttcccac
    19321 gcgagactct cattgccatg acccaaaagt acctgcgccg tccactgccg tatcaggttg
    19381 tgcgctttat tgccgcctca tcttctgact ttaatggcgc tcagggtgca gcaatattgg
    19441 cgcatcaacg ttttttgcca cagtcctgtg ctaaagtccc atgagtaaag cgttggcaca
    19501 gtagcgcaat gtcccgctgt gccaacgaca ggtacaatat atataataat tacataaaac
    19561 agcccatatt attcccagca atgatagttc tactgtactt ttccctcttc agcacaaatg
    19621 cctaataaaa tggcgcgttc agctcacact tctacttatt aaagtcgcaa tttcccaatt
    19681 atgataacgc cagctttaaa tgacgtattt tgcatggatt acatatttac gacataaatg
    19741 ataaatggag atttatatga ataactctcg gttattccgt ttgagcagga ttgttattgc
    19801 gttaactgcc gccagcagca tgatggtaaa taccgctaat gcaacagatg aagcgaaagc
    19861 cgccactcaa tatacccaac aggttaatca gaattacgcc aaatcattac cgtttagcga
    19921 tcgtcaggat tttgacgatg ctcagcgtgg atttatcgct ccgctgctgg atgaaggtat
    19981 tctgcgtgat gcgaacggta aaatttacta ccgcgcgaat gattacaaat ttgatattaa
    20041 tgccgctgcg ccggaaacca taaaccccag cctgtggcgt cagtcgcaga tcaacggcat
    20101 ttcaggcctg ttcaaagtca ccgataaaat gtatcaggtg cgcggccagg atatctctaa
    20161 cattacgttc gttgagggcg agaaaggcat tattgttatc gacccgctgg tgacgccgcc
    20221 tgcagcaaaa gccgcacttg atctttactt ccagcatcgt ccgcaaaaac cgattgttgc
    20281 cgttatctac actcacagcc acgccgacca ctatggcggc gtgaaaggta ttatctctga
    20341 agccgatgtt aaatccggca aagtgcaggt gattgctccg gcaggcttta tggacgaagc
    20401 catcagcgaa aacgtgctgg cgggtaacat catgagccgc cgtgcgctct actcttacgg
    20461 tctgttactg ccgcacaacg cgcaaggcaa cgtgggcaac ggcctgggcg tgacgctggc
    20521 aacgggcgac ccgagcatta ttgcaccgac taaaactatc gtcagaactg gcgagaagat
    20581 gattatcgac ggcctggaat ttgacttcct gatgactccg ggtagcgaag cgccagccga
    20641 aatgcacttc tatattccgg ccctgaaagc cctgtgtacc gccgagaacg ccacgcatac
    20701 cctgcacaac ttctacactc tgcgcggcgc gaaaacccgc gataccagca agtggaccga
    20761 gtacctgaac gaaacgctgg atatgtgggg taacgacgcg gaagtactgt ttatgccgca
    20821 cacctggccg gtctggggca ataagcatat caatgattat attggtaaat atcgcgatac
    20881 catcaagtac attcacgacc agaccctgca cctggcgaac cagggctaca ccatgaatga
    20941 aatcggcgac atgattaagc tgccgcctgc gcttgccaat aactgggcca gccgcggcta
    21001 ttacggttct gtcagccaca acgcccgcgc ggtgtataac ttctatcttg gctattacga
    21061 cggtaacccg gctaacctgc atccgtatgg tcaggtggag atgggtaaac gttacgtgca
    21121 agctctgggc ggttctgccc gcgtcatcaa cctggcgaaa gaagcgaaca agcaaggtga
    21181 ttaccgctgg tcggcagaac tgctgaaaca ggtgattgcc gccaaccctg gtgaccaggt
    21241 cgcgaagaat ctgcaagcgg ataactttga acagctgggc tatcaggccg agtccgccac
    21301 atggcgcggt ttctacctga ccggcgcgaa agagctgcgc gaaggggtac ataagttcag
    21361 ccacggcacc accggttccc cggacaccat tcgcggaatg tcggtcgaaa tgctgttcga
    21421 ctttatgtcc gttcgcctcg atagtgcgaa agccgcgggt aaaaatatca gcctgaactt
    21481 caatatgagc aacggcgata acctcaacct gacgctgaac gatagcgtgc ttaactaccg
    21541 taaaacgctg caacctgaag ccaacgcctc tttctacatc agccgtgaag atctgcatgc
    21601 cgtgctgacc ggacaagtca aaatggtgga tctggtaaaa gcgaagaaag ccaaaattat
    21661 tggcaatggc gcgaaactgg aagaaattat tgcctgtctg gataatttcg atttgtgggt
    21721 gaatatcgta accccaaatt aatgctactc acttttgggg gcgcttaagc ccccaaaagc
    21781 atttatttta aaaatatata caacaaatta atacataaaa catataagtt atgaatgcgg
    21841 tggctgaata aatctatttt tattcatcga gaaaatagcc atactgacgc cttcaatgga
    21901 tattgatatt cactacagat attgttcgca gcagacagga tgtatttaat atgccaacag
    21961 ttctctctcg catggcgatg caactcaaaa aaacagcctg gataattccc gtcttcatgg
    22021 tttcgggatg ctcattatct ccggcaatcc cggtgatcgg cgcttattat cccggctggt
    22081 ttttctgcgc cattgccagc cttattttga cgctcatcac gaggcgaatt attcagcggg
    22141 caaatatcaa tctggcattt gtcggaatta tttataccgc cctttttgct ctctacgcca
    22201 tgctgttctg gctggcattt ttctaatcta tttgagacgc gctcatggaa agtacgccga
    22261 aaaaagctcc tcgcagtaaa ttccctgctc tgttagtggt tgcgttggcg ctggttgccc
    22321 ttgttttcgt tatctggcgc gtagacagtg cgccatcaac taatgacgct tacgcgtcag
    22381 cagataccat tgatgtggtg ccggaagtca gcggtcgcat tgtagaactg gcggtcaccg
    22441 acaaccaggc ggtgaaacag ggtgatttgc tattccgcat cgacccacgc ccatacgaag
    22501 ccaatctggc gaaagccgaa gcctccctcg cggcgctgga taagcaaatt atgctcaccc
    22561 agcgtagcgt tgacgcgcaa cagtttggtg ccgactcggt taatgccacg gtagaaaaag
    22621 cccgtgccgc cgcgaaacag gccacagata cattacgccg caccgagcca ttactgaaag
    22681 aaggttttgt ctcagcggaa gacgttgacc gtgcaagaac tgcgcagcgc gccgcagaag
    22741 cggatcttaa tgccgtattg ttacaggcgc agtcagccgc cagcgccgtc agcggcatcg
    22801 atgcgctggt ggcgcaacgt gcggcggtcg aagcggatat tgccctgacc aaactgcatc
    22861 tggaaatggc gaccgttcgc gcgccgtttg atggccgggt catttccctc aaaacctccg
    22921 tcgggcaatt tgcttctgcc atgcgcccta tttttaccct aatcgacact cgtcactggt
    22981 acgtgatcgc caacttccgc gaaaccgatc tgaaaaatat tcgctcaggt acacccgcaa
    23041 ccattcgcct gatgagcgac agcggcaaaa ccttcgaggg taaagtggat tcgattggct
    23101 acggcgtgct accggatgac ggcggcctgg tgctgggcgg cctgccgaaa gtgtctcgtt
    23161 ctattaactg ggtccgcgtt gcccagcgtt ttccggtcaa aatcatggtc gataaacctg
    23221 acccggaaat gttccgcatc ggcgcttcgg cagtcgctaa tcttgagccg caataatgag
    23281 cgcgctcaac tccctgccat taccggtggt caggctgctg gcgttctttc atgaagagtt
    23341 aagcgagcgg cggccaggtc gcgtgccgca gaccgtgcaa ctctgggtag gctgcctgct
    23401 ggtaattctg atctcgatga cctttgagat cccttttgtg gcgttatcgc tggcagtgct
    23461 gttttacggt attcagtcga acgcgtttta caccaaattt gtcgcgatct tgtttgtggt
    23521 tgccacggtg ctggagatcg gcagcctgtt tttgatctac aaatggtcat acggcgaacc
    23581 gttgatccga ttgatcatcg ccggaccgat cctgatgggc tgtatgtttt tgatgcgcac
    23641 ccatcggttg gggctggtct ttttcgccgt cgccatagtc gctatttacg ggcaaacctt
    23701 ccccgccatg ctcgactatc cggaagtggt cgtgcgctta accctgtggt gtatcgttgt
    23761 cggcctctat ccaaccttgc tgatgacgtt aatcggcgtg ctgtggtttc ccagccgcgc
    23821 tatcacgcag atgcatcagg cgcttaatga tcggcttgat gatgccatta gccacctgac
    23881 ggacagcctc gcaccgctac ccgaaacgcg gattgaaaga gaggcgctgg cgctgcaaaa
    23941 actcaatgtc ttttgcctcg cggacgatgc caactggcga actcagagcg catggtggca
    24001 aagctgcgtg gcaacggtaa cctacattta ctcgacgctg aatcgctacg atcccacctc
    24061 ttttgctgat tctcaggcaa ttattgaatt tcggcaaaaa ttagcctcag aaatcaacaa
    24121 gctgcagcat gccattgccg aaggtcagtg ctggcaaagc gactggcgga tcactgaaag
    24181 tgaagcgatg gcggcacggg aatgtaacct ggagaatatc tgccagacgt tattgcaact
    24241 gggtcagatg gacccgaata cgccgccaac gcccgccacc aaaccgccat caatggtcgc
    24301 cgacgctttt accaatccag actatatgcg ctacgcggta aaaacgctgc tcgcctgttt
    24361 gatctgttac accttctaca gcggcgtgga ctgggaaggc attcacacct gtatgctgac
    24421 ctgcgtgatc gtcgctaatc ccaatgtcgg ttcgtcgtac cagaagatgg tgctgcgttt
    24481 tggcggggcc ttttgcggcg cgattctggc gctgttattc acgttattgg tcatgccctg
    24541 gctggacaat attgtcgaat tgctgtttgt gctggcaccg attttcctgt tgggcgcatg
    24601 gattgccacc agctctgaac gctcttctta tatcggcaca cagatggtgg tcaccttcgc
    24661 gctcgccacg ctcgaaaacg tttttggccc ggtgtacgac ctggtggaaa ttcgcgatcg
    24721 cgctctgggt atcatcattg gtaccgtggt gtcggcggtg atttacacct ttgtctggcc
    24781 tgaaagcgaa gcgcgcacgc tgccgcaaaa actggctggc gcgctgggta tgttgagtaa
    24841 agtaatgcgg atcccacgcc agcaggaagt cacagctctg cgcacttatc tgcaaattcg
    24901 cataggtctg catgcggcgt ttaatgcctg tgaagagatg tgccaacgcg tggcgctgga
    24961 gcgtcaactg gacagcgaag aacgcgcttt actgattgaa cgttcgcaaa cggttattcg
    25021 tcagggccgc gatattcttc acgcctggga tgcaacctgg aactcggcgc aggcgctgga
    25081 taacgcacta cagccggaca gagcaggtca gtttgccgac gcactggaga aatacgccgc
    25141 cggtctggca accgcactca gtcgttctcc tcaaataacg cttgaagaaa cacccgcctc
    25201 gcaggccatc ctgccgacct tattaaaaca ggagcaacac gtctgccagc ttttcgcccg
    25261 cttgccagac tggacagccc cggcattaac gcccgccacg gaacaggtac aaggagccac
    25321 gcaatgatca atcgtcaact ttcacgcctg ctgttgtgca gcattctcgg cagcacgacg
    25381 ctgatttccg gctgtgccct ggtacgtaag gattctgcgc ctcatcaaca gctcaaaccg
    25441 gaacaaatca aactggccga tgatattcat cttgccagct ccggctggcc gcaggcgcag
    25501 tggtggaaac aactcaatga cccgcagttg gatgccctga tccaacggac gctaagtggt
    25561 tcacacaccc tcgccgaagc gaaactgcgg gaagaaaaag cgcagtcgca ggccgatttg
    25621 ttagatgccg gttcacaatt acaggtcgca gcgttaggga tgctcaaccg ccaacgcgtc
    25681 tcggcgaacg gctttttaag cccttatgcg atggatgcgc cagcactggg tatggacggg
    25741 ccgttctata cggaagccac agtcggtttg tttgccggac tggatctcga tttgtggggt
    25801 gtgcatcgct cagcggttgc tgccgccatt ggcgcgcata atgccgcgct ggcagaaacc
    25861 gcagcagtag agctatcgct gaccacgggc gtagcgcagc tttattacag tatgcaggcc
    25921 agctatcaga tgctcgatct gttagaacaa acacgcgatg tgattgatta cgcggtgaaa
    25981 gcgcaccaga gtaaagtggc gcacggtctg gaagcgcaag tgcctttcca cggcgcgcgg
    26041 gcacagattc tggcggtcga taaacaaatt gttgccgtta aagggcaaat caccgaaacg
    26101 cgagaatctc tgcgtgcatt gattggcgcg ggagccagcg atatgccgga gatcaaaccg
    26161 gtggcattac cgcaagtcca gaccggcatt ccggcgacac tctcttatga gttgctcgcc
    26221 agacgcccgg atctgcaagc gatgcgctgg tatgttcagg cgtcattaga tcaggtggat
    26281 tccgcgcggg cgctgttcta tccgagcttt gatatcaaag cgtttttcgg cctggactct
    26341 atccacctgg ataccttatt caaaaaaacc agtcgccagt tcaactttat cccgggtctg
    26401 aaattgccgc tgtttgacgg tggacggttg aatgccaatc tcgaaggcac tcgcgccgcc
    26461 agcaacatga tgattgaacg ttacaaccag tcagtactga atgcggtgcg cgacgttgcc
    26521 gtcaacggca cgcgtctgca aacgctcaac gacgagcgag agatgcaggc tgaacgcgtg
    26581 gaagcaacgc gctttaccct gcgcgctgcc gaggccgcct atcagcgcgg cttaaccagc
    26641 cgcttacagg ccaccgaagc ccggttgcca gtacttgccg aagagatgtc attactgatg
    26701 ctggacagcc gccgggtgat ccaaagcatt cagttgatga aatcgctggg cggcgggtat
    26761 caggcaagtc ccgtcgtcga gaaaaaataa aatgtctgcc gcgtgatggc tgtcacgcgg
    26821 tatttcgttt cgtcacgtca aaactgacga cagcctgttt ttcgtcagag ttttgaataa
    26881 atagtgcccg taatatcagg gaatgaccac aaataaaatg tggcataaaa gatgcatact
    26941 gtagtcgaga gcgcgtatgc gtgatttgat taactggagc gagaccgatg aaaaaagtcg
    27001 tcacggtttg cccctattgc gcatcaggtt gcaaaatcaa cctggtcgtc gataacggca
    27061 aaatcgtccg ggcggaggca gcgcagggga aaaccaacca gggtaccctg tgtctgaagg
    27121 gttattatgg ctgggatttc attaacgata cccagatcct gaccccgcgc ctgaaaaccc
    27181 ccatgatccg tcgccagcgt ggcggcaaac tcgaacctgt ttcctgggat gaggcactga
    27241 attacgttgc cgagcgcctg agcgccatca aagagaagta cggtccggat gccatccaga
    27301 cgaccggctc ctcgcgtggt acgggtaacg aaaccaacta tgtaatgcaa aaatttgcgc
    27361 gcgccgttat tggtaccaat aacgttgact gctgcgctcg tgtctgacac ggcccatcgg
    27421 ttgcaggtct gcaccaatcg gtcggtaatg gcgcaatgag caatgctatt aacgaaattg
    27481 ataataccga tttagtgttc gttttcgggt acaacccggc ggattcccac ccaatcgtgg
    27541 cgaaccacgt aattaacgct aaacgtaacg gggcgaaaat tatcgtctgc gatccgcgca
    27601 aaattgaaac cgcgcgcatt gctgacatgc acattgcact gaaaaacggc tcgaacatcg
    27661 cgctgttgaa tgcgatgggc catgtcatta ttgaagaaaa cctgtacgac aaagctttcg
    27721 tcgcttcccg tacagaaggc tttgaagagt atcgtaaaat cgttgaaggc tacacgccgg
    27781 agtcggttga agatatcacc ggcgtcagcg ccagtgagat tcgtcaggcg gcgcggatgt
    27841 atgcccaggc gaaaagcgcc gccatcctgt ggggcatggg tgtaacccag ttctaccagg
    27901 gcgtggaaac cgtgcgttct ctgaccagcc tcgcgatgct gactggtaac ctcggtaagc
    27961 ctcatgcggg tgtaaacccg gttcgtggtc agaacaacgt gcagggtgcc tgcgatatgg
    28021 gcgcgctgcc ggatacgtat ccgggatacc agtacgtgaa agatccggct aaccgcgaga
    28081 aattcgccaa agcctggggc gtggaaagcc tgcctgctca taccggttat cgcatcagcg
    28141 agctgccgca ccgcgcagcg catggtgaag tgcgtgccgc gtacattatg ggcgaagatc
    28201 cgctgcaaac tgacgcggaa ctgtcggcag tacgtaaagc ctttgaagat ctggaactgg
    28261 ttatcgttca ggacatcttt atgaccaaaa ccgcgtcggc ggcggatgtc attttaccgt
    28321 caacgtcgtg gggcgagcat gaaggcgtgt ttactgcggc tgaccgtggc ttccagcgtt
    28381 tcttcaaggc ggttgagccg aagtgggatc tgaaaacgga ctggcagatt atcagtgaaa
    28441 tcgccacccg tatgggttat ccgatgcact acaacaaaac ccaggagatc tgggatgagt
    28501 tgcgtcatct gtgcccggat ttctacggtg cgacttacga aaaaatgggc gaactgggct
    28561 tcattcagtg gccttgccgc gatacttcag ataccgatca ggggacttct tatctgttta
    28621 aagagaagtt tgataccccg aacggtctgg cgcagttctt cacctgcgac tgggtagcgc
    28681 caatcgacaa actcaccgac gagtacccga tggtactgtc aacggtgcgt gaagttggtc
    28741 actactcttg ccgttcgatg accggtaact gtgcggcact ggcggcgctg gctgatgaac
    28801 ctggctacgc acaaatcaat accgaagacg ccaaacgtct gggtattgaa gatgaggcat
    28861 tggtttgggt gcactcgcgt aaaggcaaaa ttatcacccg tgcgcaggtc agcgatcgtc
    28921 cgaacaaagg ggcgatttac atgacctacc agtggtggat tggtgcctgt aacgagctgg
    28981 ttaccgaaaa cttaagcccg attacgaaaa cgccggaata caaatactgc gccgtgcgcg
    29041 tcgagccgat cgccgatcag cgcgccgccg agcagtacgt gattgacgag tacaacaagt
    29101 tgaaaactcg cctgcgcgaa gcggcactgg cgtaatacag tcctttctac agcctccttt
    29161 cggaggctgt ttttttatcc attcgaactc tttatactgg ttactcccta cccaatcgta
    29221 ttatcaaaat gaaaaaaatt atcgcattga tgttgttttt gacattcttt gcccacgcga
    29281 acgactccga gcctggcagc cagtatttaa aggcagcaga ggcaggggac cgacgcgcac
    29341 aatattttct tgccgacagc tggtttagct ccggcgattt gagcaaagcc gaatattggg
    29401 cacagaaagc cgccgacagc ggtgatgctg atgcctgcgc gctgttagcg cagatcaaaa
    29461 tcaccaatcc ggtcagtctg gactatccac aagcaaaagt tcttgcagag aaagcggcgc
    29521 aagcgggcag taaagaaggt gaagtaacgc tggcgcatat tctggtaaat acgcaagcgg
    29581 gtaaaccgga ttatccaaag gcaatttcgc tgttagaaaa cgcctcggaa gatctggaga
    29641 acgactctgc cgtcgatgcc caaatgctgc ttggtttgat ttacgccaac ggcgtgggca
    29701 ttaaggccga cgatgacaag gcaacctggt atttcaaacg cagctctgcg atttcccgaa
    29761 ccggttattc cgagtactgg gcgggaatga tgttcttaaa cggtgaagaa ggttttatcg
    29821 agaagaacaa acaaaaggcg ctgcactggt taaatctgag ctgtatggaa gggtttgata
    29881 ccgggtgtga agagtttgaa aaattaacta acggttaagg taggcctgat aagacgcggt
    29941 aagcgtcgca tcaggcattg atgtcggatg cggcgtaaac gccttatccg acctacaaaa
    30001 tcacgctaaa tcaggctatt gcgcagtctg gcgtaggcct gataagacgc ggtaagcgtc
    30061 gcatcagata ttgatgtcgg atgcggcgta aacgccttat ccgacctaca aaatcaccct
    30121 aaatcaagct attgcgcagt ctggcgtagg cctgataaga cgcggcaagc gtcgcatcag
    30181 atattgatgt cggatgcggc gtaaacgcct tatccgacct acaaaatcac cctaaatcaa
    30241 gctattgcgc agtctggcgt aggcctgata agacgcggta agcgtcgcat caggcatcca
    30301 catattgccg ggtggatatc cccggcaatc ttcaattatt gatccgcagt tttatcaaat
    30361 ttgcccagca cttcgcgctc ataagccagc gctttcttac ggtcaaattt gtgttcccac
    30421 ttggcaatca ccagcaccgc cagcgcatta cccaccacgt tcagcgcagt acgcgccatg
    30481 tcgaggatac ggtcaacacc ggcaataaac gccagacctt ccagcggaat acctacgcta
    30541 cccagcgttg ccagcaacac cacaaacgac acgcccggca cgccagcaat ccctttcgag
    30601 gtcaccatca gcgtcagcac cagaatgatt tcctgccaga tggacaggtc aatgccatac
    30661 aactgcgcga tgaaaatagc ggcaatactt tgatacagcg tcgaaccatc aaggttaaaa
    30721 gagtaaccgg tcggcaccac gaaactggtg atcgacgccg gtgctccgta ggcttccatc
    30781 ttctcaataa ttcgcggcag cacgctttca gagctggcag tggagtacgc cagaatcagc
    30841 tcatctttca gaatacgaat caggatccag acgcttaacc cgcacaggcg cgccacaatt
    30901 cccagcacta ccagcgcgaa gaacagaatg gcgaaatgca ccagcagcac cagtttcgcc
    30961 agtggccaca gagacgagaa accaaagtta gccaccgtca ccgcaatcag cgcaaacaca
    31021 cccaccggcg cataacgcat caccatgtga gtcactttaa acatggtttc agagatggag
    31081 cggaaaacgg tcaccagcgg ttcacgatgc gtcgcgggca gggatgaaag ccccagacca
    31141 aacagcaccg agaagaagat gatcggcagc atttcgcctt tcgccatcga cgccacaatg
    31201 ttcgtcggca ccagcgacaa aatcgtgccc ataatgccgt gggaactgct ttgtaccgct
    31261 tccgtagtgc tctgatattt cgagatatcg acggtcgcca gctgcgacat atccaccccg
    31321 gcaccgggct ggaagacgtt cgccagagtg atccccaaaa tgatggcgac ggtggtgatc
    31381 acctcgaagt aaataatggt tttcgcgcca atacgcccga gctgtttggc atcaccaaca
    31441 cccgcgatac ccaccaccag cgtggagatc acaatcggca caacaatcat tttgatcaga
    31501 tggatgaaga tatcacccgc cggagagagc aaattgacga ccagccagtc gcggctgtcg
    31561 ctatggtagt gcaggtagct tcccaggaga atgcccagca ccatagcaaa cagaatctgc
    31621 caggccaggc tgaattttat atttttcatc agaataactt cctcaatgga gcgccgttcc
    31681 ccgttcagaa acgcgagaac gtgacatact gccagggcat gaattgtgtt gcgttggttt
    31741 gtagattttt ttaacgctta tgatcagtat catttgcgcg gtatgttgcg caacccttta
    31801 acaacagagc ttttcgctgc attaatgcat aataacgcga gtttatggca ggattaatga
    31861 ataaccattt gttatggaaa gatttttatt tgtttaatct gcaataatac tgattggtga
    31921 ttatttttgg catatgttca ttcgattatt ttttatgcag ctcaataaca taatcttcat
    31981 caccagatga ttaatctgat ctccgctgca acaatttcgc catattaatc gactcgacca
    32041 gctgtgtccg gttgatccgg ggtgagttga gatccatcac tttttgccat agttcgatgg
    32101 cctgcgcgta attcgcctgc ataaacgcat cggaagccag cagcatcaga gcggtgattt
    32161 cgttagagtc cagcgcgagg gctttatcga tcattgcgcg tgtctgggcg gtcatatgct
    32221 ggctggcctg gtaataaagc accgtcgcca gcgccgcata cagttcagcg ttctcaccac
    32281 gtagttgcaa cgcctgacgg tacgccagca gcgaattgct gtaatcattt tgccacagat
    32341 agtactcgcc cagtaacgcc cactgttcgc tgttttgtgg attagcgcgg attttatcct
    32401 gcaatgcctg aagctgcgct tctggggttt gctggctggc aaactgatgc agcggatcgc
    32461 gctgacgctg atactccgca cgtaccgcct gccattttgg gcttaacaga taactgccga
    32521 tacagagaaa tgccatcaaa atcgtgagtg ttgtcagcat tttcaccggt atttgtgact
    32581 gtttcatcgc ttcgccctca ctcgccagag gatcagtgcc atcagcagta acaacaccac
    32641 tggcagcgcc cacaacacca gcgtctggcc cgttaacggt gggttatagc gaacaaaatc
    32701 tccgtagcgt tcggtcatcc agccgatgat ttcagcttcg ttcttcccct ccgccaccat
    32761 gctgtaaacc tgatggcgca tactgaccgc caccggcgcg ttggattcca gtaagttttg
    32821 attctggcac tgcggacaac gtaactggct ggcaatattt aacgcctgtt gctgttgttg
    32881 cggattggcg aattgccagg tgtctacgac ctgagcgcgc gcaaaacagg taaataacag
    32941 cagtagcgtg agaagtcctt tattcatcgc gcttcctccc ccgccatccg cttaacaatg
    33001 cgcccgcaat catcaacaat cctccccccc agatccagcg cacaccgctt tgtacataca
    33061 aacgaaaagc gtaacgatcc gctccggttt tctcgcccat gaccgcatac cagtcatgga
    33121 tgccgttcca gcgaattgac ggttccatca tttgctgacg gcgtgcttca taaaaacgcc
    33181 gctccggcgt cagttcacca atgcgttgct gatggtcaaa cagcgccact atcgcttttt
    33241 cgctggtgta attgcctctg gcttgcagat cgagtcgctc aaaacggaag gtgtatcctg
    33301 ccagcgtcac ctgctgaccc ggctgtaaat tcaggctgat ctcctgacga ctgacgctgg
    33361 aaaccacgat ccccgcggca aataacagca cgccagcatg agctaccagc gccggaagct
    33421 gcacgcgttt gccagagaca aacgtcgcca gcacaatcac caccagcatc aacagaccaa
    33481 acggtaacgt cgcgcggtta aaatacggcg cgccaacgga gaggcgtccc cagcccagca
    33541 gaccgtaaat catcggataa agcgttccca ccagcacgat cagtagcact gcgcaaaaca
    33601 gcaacagcgt agcgaggatt aacatttccc gcgataaccc cgaaaaacgc accgccgggc
    33661 caccgtcccg cgctcgccag ccatacagag ccagagacgc aaggctaatc agtgcaaaca
    33721 ggctgaacaa cggcacggcg cggacgttat ccagtgcgaa cgcatgaacc gaaaccagaa
    33781 tgccagaacg gacaattaag gtgcccagca gcgacagcat cagagtaact atcgccagca
    33841 gcagcgacca gtggcggaaa atcccccgct ggcgtgtcag ggacaaactg tgcagcagcg
    33901 cagtggcaga aagccagggt aataaagagg cgttttccac cggatcccag aaccaccagc
    33961 cgccccagcc cagttcgcaa taggcccacc aggaaccgag gatgatccca gccgttaatg
    34021 cactccagcc aggtaacgcc cagcgccagc aaattcgggc gcaggcacca tcaaactcgc
    34081 cgcgcagtaa actcgccagc gccacgctcg ccgccaccat caaaccgcca tagccaaggt
    34141 aaagcagcgg tggatgaaag ataagaccgg gatgttgcag catcggattg agatcgcggc
    34201 cttcgattgc tggtggaaat atccgcacga agggatcgga ccacagcact acaaacagta
    34261 gcagtgcggc gagcatgaga gataaaacgg ctagcgttag cgggaatagc ggatcggttt
    34321 gctgccgata atgccaggca aacagcgcgc tccaggcgga aagcagcagc acccaaagca
    34381 gcagcgaacc ttcatgaccg ccccacaccg ccgccagttt gagttcccac gacagcaggc
    34441 tgtagctatg ttgggcgaca taaatgaccg agaaatcgct gatgagaaaa caatacgtca
    34501 gtacgccaaa ggcgagcagc aggagcgcga actgcgccag aatgccgatg caagtgagtc
    34561 gcatcatggc aggccagcgc aaccgcactc ccgcgaaggc cgtcaacggg gtcaacacgt
    34621 tgaccccgag acttaacaac aacgccagaa aaccgacttc aggaaggaat acgtccagaa
    34681 accacctctt ttctggctta cgccaccgtc aactgtccgg cgtagagaat gaaaaagcgc
    34741 agcatcaaca cgccgaccag actggcccca cacgccgcca gcacggtagg aatgccggaa
    34801 ctgcgattga cccacggttt gagcaacatt ggcacaatca gccccagccc ggcgacacca
    34861 agccagaacc accacgtcca gaaaccgcca cctaatgccg ccaccagcgc acgcacttta
    34921 ccgtcatcgc ccagcgccag accgacaaaa aacgccacca gcaggaagat ttcaccccat
    34981 accacaggga tttccatacg gtgaacaaag tgcgcttccg tggaatgcgg atttttgcgt
    35041 tggcgtatcg ccatggcgat cagcgctacc gccgcaccgg acgagatgcc ggagaagagg
    35101 aacagcaccg gcaggatcgg gttattgagg aacggatacg atttcagcgc cgacagcaga
    35161 aagccggtat aagcccccag cagcaccgcc agcaccagca tcaatgtttc cagtccgcga
    35221 tgaacgggcg tcagcaggct cagaaccttt tgcacgatcc ccagcttcgg caaccagcgt
    35281 tgttgcaggg caagcaaatc atgttcaaag attttcgcca gccacagcac cagcaccacc
    35341 atgtagagct gaaacaacat cacccccatc gacatcaccg aggtaaaact gtagtggaac
    35401 atcagcttcc agaaggtcca cggctttgtc aggtggaaga cgaggatcaa cagaccgagg
    35461 atcaccgcgc caggcccgac aattagcgtg gtgcgcagca acgtactgtc tgcaccgccc
    35521 gcctgcgggt agaagcgacg taacagcacg gccagcgtca ccagaccggc agaaatacca
    35581 atcaaaaaca ggtagatggc aatcggccag tcccacacca gcgattcaaa atgaaatgcg
    35641 gaagtctgca tcattggctc acctccccgt atttaaacgg tacgcggtac agcttcggtt
    35701 tggttcccag cgccagcttg tagcggtaag tgggcttctg gcgcagcagt tgcgaaatct
    35761 cactgttggg atcgtccaga ttgccaaacg tcagtgcctt ggtcgggcaa gcttcaacgc
    35821 acgcgggcag cttaccggcc tgcaaattgg tcttacggca gaaatcgcat ttgtccgccg
    35881 tcttcgtgac cggatggata aagcgcacgc ggtacggaca ggcggcgatg cagtactgac
    35941 aaccgacgca gagatcagga ttaacatcga caatcccgct agcggcatca cgaaaagacg
    36001 caccggtcgg gcagacgtca acgcacggcg catgatcgca gtgctggcaa gacttacgga
    36061 agaagcgata tttcacatca ggaaattcgc cctgcggctc gctacgaata atcgtcaggc
    36121 gcgagacgcc ttccggcacc ttgttcactt cccgacaagc atccatacag gcggtacagc
    36181 cgatgcataa cgactcgtca tgcaccatgc cgtaacgcac cccattgata ttcaacgttt
    36241 tcgccaccac gcgccctgcg gtgccgctga cggctgccag cacgccgacg ccggtgagaa
    36301 actggcgacg agaccaggtc atggctgctc cttaagcaac ggaacagacg ccgggttaaa
    36361 gttcggattg gtgcgctgat cgctgtggca atcgacgcaa atcttaatcc gtcctttgtc
    36421 acttaacgtc tgcatcgtat cttgctgtgg atgcagggaa tggcagctgg cgcacgccac
    36481 tttggttacg tggacatcgt gtggccagaa cgctttttgc aactgttccg gcagatggca
    36541 ggacatacag acgctgttct gctctccaac cttgtacatc ggctcgttaa agcgcatcac
    36601 atctttcacc ccttcgcggt gttgcggtga tggctgaccg tggcagttgg tgcaggtgac
    36661 cggcagtttg ttattcgggt tgataacgga agcatgtttg ccatgcatac cttcggtatc
    36721 tggcttatga cagtccagac aggcggcatc cgggttacgc tgctgggtaa cttcgtagcg
    36781 atcaccagat gcctgcgccg caggggtagc ggtaattccg ttcaggctcc acaacaggcc
    36841 cgacgccagc accccggcag ttaataacga acgtaatacg ctcatattca ctccattgtg
    36901 ttttccaccc cttgcgaggc ggaacggggt tattggctta acagaccgtt tttacgtgcc
    36961 tgctcttccc actgcgggat caccgttttg atgaagtcct gcttctcggc cttgatttgc
    37021 tccatgttca ggccaatggc ctgctgggct ttctctttgg tcgagatatc cgggatctgg
    37081 atttcatggg taatgccttt ggtcgccagc aggcgcgcca gtttggtgcg tgcatccgcc
    37141 gctttatcca tcgccgtacc gagcatcctc agaccttctt ccggtgcgtg catatgaatg
    37201 ccgtgggaag cgatcgccaa atcccaacgc cactgggcat gacggatatc gtcctgaatc
    37261 ggcttcattt cggcttccgt tgcgcctgca tccagcgccg ctttcgcttc gaagtgagcg
    37321 tgaaccagtt gatcttcaac cttgattttc aggtcgttaa tcgactgctt acgttccgcg
    37381 accacttttt gcaaggcagc tttgtcctgg gtatggcagt tcgcacaagt ctgggcgaag
    37441 ttatcaaacg gattaccaat tttatggtcg gtgtagagtt tgccttcggc gttctgcact
    37501 tttggcatat ggcaatcgat acaggtcacg ttgtttttgc cgtgaatgcc cgctgtccag
    37561 gtttcatatt ccgggtgctg cgctttcagc attggcgttt tcgacaggga gttagtccag
    37621 tcagagaagg caattttgtc gtaatactgc tccatatttt cgactttcat gccgtcatcc
    37681 cacgggaatt taaccgcttt gtttttgccg tcgaagtaat actccacatg gcactgaccg
    37741 caaaccatcg attgctggtc gaaacgtccg gctttctcaa aaggtttacc aatggcttcc
    37801 atcgcgcgag ccgcatacgg acgggaaagg gttaactccg gtttgccttt ggcgaactct
    37861 ggtgaggcgg tgttatggca atcggcacaa cctaagttgt tgacgatttc cgggccgccg
    37921 cgcgcccatt ttccgtggaa gtagccatct tcgccgtctt tctggatcag acgcgccaca
    37981 tctgggcttt tacaactcca gcatgccatc ggtagcggac catcttcagc gtttttcggc
    38041 gcaccggtac gcagggtttc acgcacatcg gtcacagcaa aggcatgtcc acgcggcttg
    38101 ttgtaatcgc gcgagaaggg ataccccgcc cacaggatca ccagccgtgg atcttccgcc
    38161 agggcgtcaa cacgctctga ctgttccgag gtggctttcc aggagagata ttgatcggga
    38221 tgttgcgggg caaaggtttc attcttcgct tcaacagtta caggttttgc aggagctgcc
    38281 gtttgttcag cgtgaacaga agtgaaaaag aaaaaaggaa tcaataagct gaagatacgg
    38341 cgtgcgttta tttttatcct tgtcataggg gcttcatccg aattgcgcca ttgttgcaat
    38401 ggcggttttt attgtttttc acgacagtaa ccgcacctac actgtcatga cattgctcgc
    38461 ccctatgtgt aacaaataac cacactgtga atgttgtctt taatcaattg taagtgcatg
    38521 taaaatacca ctttagagtt agtcagtatc ttcctctttt tcaacagcat gcataactgc
    38581 atgttcctca aagaattaat caacttttgt tgctgacctt caaaaattac cctgccgttt
    38641 atttgcacaa ttctactttt gcgtgatctg tcgcccaaat actaaacaaa actgccaata
    38701 cccctacatt taacgcttat gccacatatt attaacatcc tacaaggaga acaaaagcat
    38761 gagtcaaatt cacaaacaca ccattcctgc caacatcgca gaccgttgcc tgataaaccc
    38821 tcagcagtac gaggcgatgt atcaacaatc tattaacgca cctgatacct tctggggcga
    38881 acagggaaaa attctcgatt ggatcaaacc ttaccagaag gtgaaaaaca cctcctttgc
    38941 ccccggtaat gtgtccatta aatggtacga ggacggcact ctgaatctgg cggcaaactg
    39001 ccttgaccgc catctgcaag aaaacggcga tcgtaccgcc atcatctggg aaggcgacga
    39061 cgccagccag agcaaacata tcagctataa agagctgcac cgcgacgtct gccgcttcgc
    39121 caataccctg ctcgagctgg gcattaaaaa aggtgatgtg gtggcgattt atatgccgat
    39181 ggtgccggaa gccgcggtcg cgatgctggc ctgcgcccgc attggcgcgg tgcattcggt
    39241 aattttcggc ggcttctcgc cggaagccgt tgccggacgc attatcgatt ctaactcacg
    39301 actggtgatc acttccgacg aaggcgtgcg tgccgggcgt agtattccgc tgaagaaaaa
    39361 cgttgatgac gcgctgaaaa acccgaacgt caccagcatt gaacatgtgg tggtactgaa
    39421 gcgtactggc gggaaaattg actggcagga agggcgcgac ctgtggtggc acgacctggt
    39481 tgagcaagcc agcgatcagc accaggcgga agagatgaac gccgaagatc cgctgtttat
    39541 tctctacacc tccggttcta ccgggaagcc gaaaggcgtg ctgcacacta ccggcggtta
    39601 tctggtgtac gcggcgctga cctttaaata tgtctttgat tatcatccgg gtgatatcta
    39661 ctggtgcacc gccgatgtgg gctgggttac cggacacagt tatttgctgt acggcccgct
    39721 ggcctgcggc gcgaccacgc tgatgtttga aggcgtaccg aactggccga cgcctgcccg
    39781 tatggcgcag gtggtggaca agcatcaggt caatattctc tataccgcgc ccacggcgat
    39841 ccgcgcgctg atggcggaag gcgataaagc gatcgaaggc actgaccgtt cgtcgctgcg
    39901 cattctcggt tccgtaggcg agccaatcaa cccggaagcg tgggagtggt actggaaaaa
    39961 aatcggcaac gagaaatgcc cggtggtcga tacctggtgg cagaccgaaa ccggcggttt
    40021 catgatcacc ccgctgcctg gcgctaccga gctgaaagcc ggttcggcaa cacgtccgtt
    40081 cttcggcgtg caaccggcgc tggtcgataa cgaaggtaac ccgctggaag gggccaccga
    40141 aggcagtctg gtgatcaccg actcctggcc gggtcaggcg cgtacgctgt ttggcgatca
    40201 cgaacgtttt gagcagacct acttctccac cttcaaaaat atgtatttca gcggcgacgg
    40261 cgcgcgtcgc gatgaagatg gctattactg gatcaccggg cgcgtggacg acgtgctgaa
    40321 cgtctcgggt caccgtctgg gaacggcgga gattgagtcg gcgctggtgg cgcatccgaa
    40381 aatcgccgaa gccgctgtcg tcggtattcc gcacaatatt aaaggtcagg cgatctacgc
    40441 ctacgtcacg cttaatcatg gggaggaacc gtcaccagaa ctgtacgcag aagtccgcaa
    40501 ctgggtgcgt aaagagattg gcccgctggc gacgccagac gtgctgcact ggaccgactc
    40561 cctgcctaaa acccgctccg gcaaaattat gcgccgtatt ctgcgcaaaa ttgcggcggg
    40621 cgataccagc aacctgggcg atacctcgac gcttgccgat cctggcgtag tcgagaagct
    40681 gcttgaagag aagcaggcta tcgcgatgcc atcgtaaccc acaattgccc gatgcgagtc
    40741 ggtaacggtt tgtaggcctg ataagacgcg acagcgtcgc atcaggcgct ggttgccgga
    40801 tgcggcgtga acgccttatc cggcctacat tcggcaacgg ctgtaggcct gataagacgc
    40861 gacagcgacg catcaggcgt tgattgccgg atgcggcgta aacgccttat ccggcctaca
    40921 ttcggcaacg gctgtaggcc tgataagacg cgacagcgtc gcatcaggcg ttgattgccg
    40981 gatgcggcgt gaatgcctta tccggcctac attcggcaag ggttacccaa gcgttaacct
    41041 tctcccataa gggagcggga attaaaacaa tccctacatt acctctggag aatctgtgat
    41101 gaatggtacg atttatcagc ggatagaaga caatgcgcat ttcagggagt tagtcgaaaa
    41161 acggcaacgg tttgccacca tcctgtcgat tattatgctg gcagtttata tcggctttat
    41221 tttactgatc gccttcgcgc ccggctggct gggcaccccg ctgaatccga acaccagcgt
    41281 cacacgcggt attccgattg gtgttggagt aattgtgatc tcctttgttc tcactggtat
    41341 ctacatctgg cgggcgaacg gcgaattcga ccgtcttaat aatgaagtcc tgcatgaggt
    41401 acaagcatca tgaaaagagt tctgacggcg cttgccgcca cactcccttt cgcagctaac
    41461 gccgcggatg ctattagcgg ggccgtagag cgccagccaa caaactggca ggcgattatt
    41521 atgttcctga ttttcgtcgt gtttacgctc ggcattacct actgggcgtc aaaacgcgta
    41581 cgttcgcgta atgactacta caccgcaggc ggcaatatca ccggttttca gaatgggctg
    41641 gcgattgccg gggactatat gtccgccgcc tcattcttgg ggatctccgc gctggtgttt
    41701 acctccggct atgacggctt aatttactcg ctgggcttcc tggtaggctg gccaatcatt
    41761 ctgttcctga ttgccgaacg tctgcgtaac ctgggccgct acacctttgc cgatgtagcc
    41821 tcttaccgcc tgaaacaagg gccgattcgt attctttcgg cctgtggttc tctggtggtg
    41881 gtggcgcttt accttatcgc ccagatggtg ggcgcaggta aactcatcga gctgctgttt
    41941 ggccttaact atcacattgc ggtggtgctg gtcggcgtgc tgatgatgat gtacgtcctg
    42001 ttcggcggca tgctggcgac cacctgggta cagattatca aagccgttct gttgctgttc
    42061 ggtgccagct ttatggcctt tatggtgatg aaacacgtcg gctttagctt caacaatctg
    42121 ttcagcgaag cgatggcggt acacccgaaa ggtgtcgata tcatgaagcc aggcgggctg
    42181 gtgaaagatc cgatctcagc actgtcgctg gggctcggac tgatgtttgg tacggcgggc
    42241 ttgccgcata ttctgatgcg cttctttacg gtcagcgatg cccgcgaagc acgtaagagc
    42301 gtgttctacg ccaccggatt tatgggctac ttctacatcc tgacctttat tatcggcttc
    42361 ggcgcaatca tgctggttgg tgcgaatccg gaatataaag atgctgcggg ccatctgatt
    42421 ggtggtaaca acatggcggc agttcacctg gccaatgcgg tgggcggcaa cctgttcctc
    42481 ggctttattt cagcggttgc tttcgccact atcctcgcgg tggtagcggg gctgacgctg
    42541 gcgggcgcat cggcggtgtc tcacgacctg tacgctaacg tcttcaaaaa aggcgcgacc
    42601 gaacgtgaag agctgcgggt atcgaaaatc accgttctga ttttgggtgt gattgccatt
    42661 atcctcggcg tgctgtttga gaatcagaac atcgccttta tggtggggct ggcgtttgcc
    42721 atcgcggcga gctgtaactt cccgatcatt ctgctttcca tgtactggtc gaaactgact
    42781 acccgtggcg caatgctggg cggctggtta ggactgatta ctgcggtggt gctgatgatc
    42841 ctcggtccga cgatttgggt acagatcctc ggtcacgaaa aagccatctt cccgtatgaa
    42901 tacccggcac tgttctctat cagcgtggca ttcctcggta tctggttctt ctcggcaacg
    42961 gataattcag cggaaggtgc gcgcgagcgt gaactgttcc gtgcgcagtt tatccgctcc
    43021 cagactggct ttggcgttga gcaaggccgc gcgcattaat ctccactagc ctctggtcac
    43081 ctgaccaacg gtaaaaaggt gcgatgtact gcaccttttt accgattaca ccgccaaagc
    43141 gttattggtt ctcttcaatc agcaacgctt caagcaaatc gagatcgtgc agtaattttt
    43201 gcagcgtttc gttactgatc tcccgcgtgg cgcgcaggtg gtacagttca gcacgttcag
    43261 aacgcaatgc cgccagacgg aagcgacgct caaggttctc ttcctgcacg gaactttcaa
    43321 cgtcgttacg tccatcggcg cgacgacgca ggttaccaat gacgcgagaa ctaacctctg
    43381 tgagcagctg gttatcgata ttctcttcgg tatccgccgc cagacgctcc tccattttct
    43441 ggatcgccac aatcgccact tctgccgttg ccgctcgcgc aatacgttcc tctttcaatt
    43501 gttgcgaatg gtccgcgact tcaatgtgtt gtagcagaat aggcaacatc accacgccga
    43561 caaacagcga gaagagaatg acaccagccg ccaggaacac cagctcatag cgcgccggga
    43621 agacgttacc atccggcaag agcagcggga ttgagagcac accagccaga gtgatcgccc
    43681 cacgcacccc ggcgaaagac gcgatcagga tttctcgtgt ggtccacgaa ccaaactcca
    43741 tcggcttctt cttcaggaag cggttgctga actttttcat cgtccacagc cagccgaaac
    43801 ggaccagcat cagcgccgca taaatcagaa tgatatcggt aaacagcatc cagatttcga
    43861 cgttagggtc gatttctgcc gccatcagcg acgtctccag aatacccggc agctgcagac
    43921 ctaacagcag gaataccatg ccgttaaaga caaattccag catcgcccag gtactgtttg
    43981 cgcgcaggcg cattgccagc ggcgcacggc gcatcacacc ggaacgggtg atggtcatcc
    44041 cggcggcaac cgcagcgagg ataccggaaa cgccaatatg ttcggcaatc agataagaag
    44101 cgaatggcag caggaacagc aggacgatct gcgtcgcggg ttcatcaccg ccccagcggc
    44161 tgaggaatcg cagcgagcga ccgtacagcc agctcaccac aaaaccggcg agaataccgc
    44221 caatggccac tttcataaat tcgaccgtcg caccaccgac ggtgaagatc atcgtcccca
    44281 tcgccactgc cacggcaaac ttcaacgaca ccagaccgga ggcgtcattc atcaacgctt
    44341 cgccctgcaa aatgcccatg atttttttcg ggatgcgccc ttcgccaaca atccccgaga
    44401 gcgccacagc atcggtcgga gaaagcaccg ccgccagcgc aaaggcgggg atcagcggaa
    44461 tgcccggcac cacccagtaa ataagaaagc caatgccgac cacggtgacc accaccagcg
    44521 ccagcgcgag gccgaaaatc tctcgaccat gttcgagaaa ttcacgggtc ggcgttttcc
    44581 agccatcagc gaacagcaac ggcgggataa acaagactaa aaagagttca ggatcaaact
    44641 ccacatgcaa accaaacgtc ggccacgcca gtagcgcacc gatggcgatt tgcataagcg
    44701 gaagcgggat ctgaaagggc atgacacgag tgaccacccc ggacagcgag accacgaggg
    44761 tcattatcag tatggtgaag aagatttcca tgcgttccct gtttgcgatg ttttttatga
    44821 tctgagaaag gcgttatgcc tgaattctca ttttttcaga atatcgcaaa agctcacaca
    44881 atgtgcggag aaaataaaaa cgggcagcca ttgccacccg ttttcagaat catatgaaag
    44941 attagatagc ccagccaccc gcatagaagg tcaccagcgc cacagcgata acgaccgtac
    45001 cgatgttcaa cttgcgccat tcgccggaaa ccagacgacc aatcaccaga gtcgcgaagc
    45061 cgatcatgat gcctgttacg atgttacagg tcagcacgat gaataccgcc gtaaccagac
    45121 ccgccatcgc atcaacaaaa tcagcaaagt cgattttcgc cacgttactc agcatcagca
    45181 ggccaacgta catcagcgcc ggagccgttg cataccccgg aacgaggtaa gagagcggag
    45241 agaggaacag aatcagcagg aacagcacgc caacggtgat agccgtcaaa ccggttttac
    45301 cgcccgccgc cgtacccgcc gcagactcga tgtataccgc tgccggagct gcacccacca
    45361 ggccagagaa aacgctgctc atggagtcag tggtcagtgc tttcccgccg tcgatgatct
    45421 gcccatcttt atccagcagg ttcgcctggc cggcgacggc acggatagtt ccggtggcat
    45481 caaatactgc cgtcatcacc agcgccagaa cgcttggcag gactacagga ttcagcgcgc
    45541 ccataatgtc caggctgcca attagtgaat tgccgttttc atcgctcaat gaaggcatgg
    45601 cgaaaacgcc ggagaaatgg acgttaggat cgaagatcaa accgacaatt gagataccga
    45661 taatggtcag cagaatgcca ccagggactt tcagtttttc caggccgata atcactgcca
    45721 gccccaccaa cgacatcatc accggaaagg tcgtgaaatc ccccagcgcg acgggcagac
    45781 catcaagtgg gtttttaatc accagaccga caccattagc agcaatcagc agcaggaaca
    45841 gaccgatacc aatccccgtg ccgtgcgcca caccgtgcgg caagttgcgc aaaatccagc
    45901 tacggatacc cgtggcagaa atgaccgtaa acagcacacc catcaggaac acggcaccca
    45961 gcgcgacagg tacgctaata tgttgcccca gcaccagact gaatgcggta aacgccgtca
    46021 gggagatggc gcaaccaatc gccaacggca gattagccca cagacccatc acgatagaac
    46081 cgagtccggc aaccagacag gtcgcaacga aaactgccgc aggcgggaag cctgctttac
    46141 ccaacatgcc tggaacgacg ataaccgagt agaccatcgc cagaaatgtt gttaacccgg
    46201 caaccacttc ctgacggaca gtgcttccac gttgtgaaat tttaaaccag gcgtcgagtg
    46261 aaccgccggt acgcgctgat ggcgtagaca taagaaaaca tcccctgaga gtttaatttt
    46321 cgtcaagatg cgtggcggac aatcccctgc cagcgaaacg ttatcttcct gctccaggtt
    46381 tgcgacaaat tggtgcgtcg caaaaaagca aacgtttagc tccgcgcata caaaacgggt
    46441 ggtaaaaaaa gcaaacgatt atccggcgat catagcggct ttttcaaccg aagtttgctg
    46501 cttttttctc ttttattgtc ttgacgatga aaaaccaggc aattgatgcg caaacgttat
    46561 cgtcgatgcg caagattgca gcgttatcga gccgtatacc gttcaggatt gcgataaaaa
    46621 atcgtctgag atgacaaaaa tattgcggct aaaaagtgta cttttacact ggcgaagaaa
    46681 cgccggtgac atacaattaa agcatcaaca ccaaccggaa cctccaccac gtgctcgaat
    46741 gaggtgtgtt gacgtcgggg gaaaccctcc tgtgtaccag cgggatagag agaaagacaa
    46801 agaccggaaa acaaactaaa gcgcccttgt ggcgctttag ttttgttcat cttccagcaa
    46861 gcgtgcgcct gtgccttctt ctcctaagcg gtcgcccggg ttacgcaatg ggcaatcact
    46921 gcgcgaaagg cagccacaac caatacatcc gtccagctcg tcacgcagcg ccactaaggt
    46981 atgaatgcgc cgatccaact cttctcgcca ttgggacgaa agctgtttcc actctttcgc
    47041 acttaatgta tgcccttcgg gcaacacgcc aaatgcttca cgaatggtcg ccagcggaat
    47101 gccaatacgc tgagcaattt tgatgattgc gacatatcgc aacacatcac gtttatatcg
    47161 ccgctgattg ccactattac ggatactgct aatcaaccct ttgctttcat agaaatgcag
    47221 cgccgatacc gccacaccgc tgcgttttgc cacttcgccg ggggttagca gcgctttaat
    47281 gcggggtaat ttcttttcca taaatcgctt tacctcaagt taacttgagg aattatactc
    47341 cccaacagat gaattaacga actgaacact gaaaagaggc agatttatgt cccatcagaa
    47401 aattattcag gatcttatcg catggattga cgagcatatt gaccagccgc ttaacattga
    47461 tgtcgtcgca aaaaaatcag gctattcaaa gtggtactta cagcggatgt tccgtactgt
    47521 gacgcatcag acactaggtg attacattcg ccaacgccgc ctgttactgg ccgccgttga
    47581 gttgcgcacc accgagcgtc cgatttttga tatcgcaatg gacctgggtt atgtctctca
    47641 gcagaccttc tcccgcgttt tccgtcggca gtttgatcgc actcccagcg attatcgcca
    47701 ccgcctgtag ttttattgcc cgcgcgttaa ctcccgcgcg ggtaattgct ccatccattg
    47761 cataaacacc tgcggcggca tcgccttcgc aaagaaccat ccctgacaat agcgcacgcc
    47821 gcgtttgcgc agccagttaa cctgctcctc agtttcgacg ccttcagcga tcgtttttaa
    47881 ccccagactg tgcgccagct cgatgatatg ttccgcaatc aaatgactgg ttttatgggt
    47941 ggtcagcgtc tcaacaaacg atttgtcgat tttcaaaata tcgacattca atgatttaag
    48001 gttatgcaag ttagagtagc caataccaaa atcatcaatt gccacttcgt aaccagcctg
    48061 gcggaaagcc agaataatcg gcgtcatttt gtcgacatca agaaatgcat gttcagtcac
    48121 ttcaaattta atttgctgcg gacgcaccgc atattgctct gttttctggt tggttcgcgc
    48181 tatcaaccgt gacgtatgaa aatcggaggc cgacaggtta atagaaacat agcgatcgac
    48241 atgtgttgcc aggtacacgc ccagatcgcg gaagacatta tcaataacat aatcagttat
    48301 ctgttcgatc atcccctcct tttctgccag cggaataaac tctgccggat tcattacttg
    48361 cccctgctca ccaggccaac gtaacaatgc ttcagctccg atacattttt ctgttttgat
    48421 atcgattatt ggctggtaat aaaggcaaag ttgatgtttt tcgagggcgc gttgcaattt
    48481 gcgtttggga gacaaatagt tttgtcgaat acgtagccag agtaatagca gaacaagact
    48541 ccccaaaata ccggcgggca acgcaaaaat aagatgatta taaaaatgag ttataagacg
    48601 ttgatatgaa gtcgcaacaa tggctgcaat tgggcgtttt gtcgaataaa ctgtcgcata
    48661 taaatagcca tttcgttgta cggttaaatc attcaaatga atcagcggag aaaacgttgc
    48721 tgccgaggcc tcgttgctta acgagaaaaa ggttttcgtc accgtatcat acactcccca
    48781 ttgcaatgtc ggatcatcag acatcacttc actccagaag agagggttga taaccgccac
    48841 ataatttccc cgctgcatgt aggtcatttt atagccagag aaaaaaggcg tatcacggta
    48901 ataatagata gaaacgttag gttcacgctt ataatcggcc ggtgcaatcg tatagccatt
    48961 tacagacgct atcagcgatg agcataaaaa atggttatca cgggcataga tcaattcatt
    49021 aatataaaga tagccacgaa taatattcaa cattcgcttt tgatgggctg gagtgcaaac
    49081 ttgcccctga tagcgttctg ctgcatcgct cactaaatct gcctggcgaa tgaccagctc
    49141 ggatttatcc agcgccagct gagcaaaagt ttggagctga ctattcactt ctgattttgc
    49201 ccaaaggaag gcaatccata gcgacagaat gatgggaaag agaactaaaa agataatgcc
    49261 cggcaacgcc agtaattggt gtcgtgcacg atgactcatt gctatcaatt tcctgcctgt
    49321 ttatacccgg ccattgcaag gtgaaaaccc tgggcaccga cgtctttaga taaatcttac
    49381 ggtataaata gcacttttgc ctgatttgaa attgatacct ttttaatggt cacctggata
    49441 aaaataattc attgatattc aaaactaatt ttctacagga atgcagaggc ggcgggaaga
    49501 tatgccctta atgccactga aaaaccacat aatctgtaca gcaataaaag tcacggccta
    49561 atctggctaa caaacaatca attgtgatat agttcacaaa attaatgaaa caaacagagt
    49621 gtttcatttt tgtgtcctgc aagccccgcg atagccagag tgtaccacgc ctcccgtgaa
    49681 caacgccgcg ctgtccaggg catcggctct tttcagagga attgtttgat ggctaccctc
    49741 accactggcg tggttctact tcgctggcaa cttcttagtg ccgtaatgat gtttctggcc
    49801 agcacgctca acatccgttt tcgtcggtct gattatgtcg ggcttgcagt gatcagcagc
    49861 ggtctgggcg tggtttctgc ctgctggttc gcaatggggt tgcttggcat cacaatggcg
    49921 gatatcaccg ccatctggca caacatcgag tcggtgatga ttgaagagat gaatcagaca
    49981 ccgccacaat ggccaatgat tttaacttga tatgtagaag cctccataat ggaggcttct
    50041 ttttttatgg ctggcagatg ctttaatcat ccaccttaaa acaatataac ctattgtttt
    50101 aatgacaaat cagaacggaa tatcgtcatc aaaatccatc ggcggctcgt tagacggtgc
    50161 tgccggagcg gactgctgcg ggcgagactg cgcgccgccg ctgaactgat tgccgccctg
    50221 cggctgctga ggctgacccc aaccgccctg cggctgacca ccaccgatat tgccacctgc
    50281 aggagcgcca ccaccctgac gaccacccag catctgcatg gtgccgccaa cgttcaccac
    50341 gacttctgtg gtgtagcgat cctgaccgga ttgatcggtc catttgcggg tacgcagctg
    50401 accttcgata taaacctgag aacctttacg cagatattcg ctggctactt ctgccagttt
    50461 gccgaacagc acaacgcggt gccactcagt ctgctctttc atctcgccgg tcgctttgtc
    50521 acgccaggat tcggaagtag ccagcgtaat gttggcaaca gcgccaccat ttggcatgta
    50581 gcgtacttcc gggtcctgac ccagattacc aacgagaata accttgttta cgcctctgct
    50641 ggccatgttc gtgtctcctg aaaaaaaatc gttctgaata agtgtaaacg cgcgattgta
    50701 ccattaccaa tagcgctttt actatgttgt gacctcggtt ccgggaaaca aacctggcca
    50761 gacattgtta cacaacactc cgggtaatgc attccaatac tgtatattca ttcaggtcaa
    50821 tttgtgtcat aattaaccgt ttgtgatcgc cggtagcacc atgccaccgg gcaaaaaagc
    50881 gtttaatccg ggaaaggtga atggataaga tcgaagttcg gggcgcccgc acccataatc
    50941 tcaaaaacat caacctcgtt atcccccgcg acaagctcat tgtcgtgacc gggctttcgg
    51001 gttctggcaa atcctcgctc gctttcgaca ccttatatgc cgaagggcag cgccgttacg
    51061 ttgaatccct ttccgcctac gcgcggcagt ttctgtcact gatggagaag ccggacgtcg
    51121 accatattga ggggctttct cctgccatct caattgagca gaaatcgacg tctcataacc
    51181 cgcgctctac ggtggggaca atcaccgaaa tccacgacta tttgcgtctg ctgtacgctc
    51241 gcgttggtga gccgcgctgc ccggaccacg atgttccgct ggcggcgcaa accgtcagcc
    51301 agatggtgga taacgtgctt tcgcagccgg aaggcaagcg tctgatgctg ctcgcgccaa
    51361 tcattaaaga gcgcaaaggc gaacacacca aaacgctgga aaatctggca agccagggtt
    51421 acatccgtgc tcgtattgat ggcgaagtct gcgatctttc cgatccgccg aaactggaac
    51481 tgcaaaagaa acataccatt gaagtggtgg ttgatcgctt caaggtgcgt gacgatctca
    51541 cccaacgtct tgccgagtcg tttgaaaccg cgctggagct ttccggtggt accgcggtag
    51601 ttgccgatat ggacgatcca aaagcggaag agctgctatt ttccgccaac ttcgcctgcc
    51661 caatttgcgg ctacagtatg cgtgaactgg aaccacgcct gttttcgttt aacaacccgg
    51721 cgggtgcctg cccgacctgt gacggccttg gcgtacagca atatttcgat cctgaccgcg
    51781 tgatccagaa tccggaactg tcgctggcag gtggtgcgat ccgaggctgg gatcgccgca
    51841 acttctatta cttccagatg ctgaaatcgc tggcagatca ctataagttc gacgtcgaag
    51901 cgccgtgggg cagcctgagc gcgaatgtgc ataaagtggt gttgtacggt tctggcaaag
    51961 aaaatattga attcaaatac atgaacgatc gtggcgatac ctccattcgt cgtcatccgt
    52021 tcgaaggcgt gctgcacaat atggagcgcc gctataaaga gacggaatcc agtgcggtac
    52081 gtgaagaatt agccaagttt atcagcaatc gtccgtgcgc cagctgcgaa gggacgcgtc
    52141 tgcgtcggga agcgcgccac gtttatgtcg agaatacgcc gctacctgct atctccgaca
    52201 tgagcatcgg tcatgcgatg gaattcttca acaatctcaa actcgcaggt cagcgggcga
    52261 agattgcaga aaaaatcctt aaagagatcg gcgatcgtct gaaattcctc gttaacgtcg
    52321 gcctgaatta cctgacactt tcccgctcgg cagagacact ttctggcggt gaagcccagc
    52381 gtatccgtct ggcgagccag attggtgcgg gcctggttgg cgttatgtac gtgctggacg
    52441 agccgtctat cggcctgcac cagcgcgata acgagcgcct gctgggtacg cttatccatc
    52501 tgcgcgatct cggtaatacc gtgattgtgg tggagcacga cgaagacgcg attcgcgccg
    52561 ctgatcatgt gatcgatatc ggtccgggtg cgggtgtaca cggcggtgaa gtcgtcgcgg
    52621 aaggtccgct ggaagcaata atggcggtgc cagagtcgtt gaccgggcag tacatgagcg
    52681 gtaaacgcaa gattgaagtg ccgaagaagc gcgttccggc aaatccggaa aaagtgctga
    52741 aactgacagg cgcacgcggc aacaacctga aagacgtgac gctgacgctg ccggtgggtc
    52801 tgtttacttg catcacaggg gtttcaggtt ccggtaaatc gacactgatt aacgacacac
    52861 tgttcccgat tgcccaacgc cagttgaatg gtgcgaccat cgccgaaccg gcaccgtatc
    52921 gcgatattca gggactggaa catttcgaca aagtgatcga tatcgaccaa agcccaattg
    52981 gtcgtacccc acgttccaat ccggcaacct ataccggcgt gtttacacct gtacgcgaac
    53041 tgttcgcagg cgtaccagaa tcccgtgcgc gtggttatac gccagggcgt ttcagcttta
    53101 acgtccgtgg cggacgctgc gaggcctgtc agggcgacgg cgtgatcaaa gtggagatgc
    53161 acttcctgcc ggacatttac gtaccgtgcg accagtgcaa aggcaaacgc tataaccgtg
    53221 aaacgctgga gattaagtac aaaggcaaaa ccatccacga agtgctggat atgaccatcg
    53281 aagaggcgcg tgagttcttt gatgcggtgc cagctctggc acgtaagctg caaacgctga
    53341 tggatgttgg cctgacgtac attcgcctcg ggcagtccgc aaccacactt tctggtggtg
    53401 aagcccagcg cgtgaagctg gcgcgtgagc tgtcaaaacg cggcaccggg cagacgctgt
    53461 atattctcga cgagccgacc accggtctgc acttcgccga tattcagcaa ctgctcgacg
    53521 tactgcataa actgcgcgat cagggcaata ccattgtggt aattgagcac aatctcgacg
    53581 tgatcaaaac cgctgactgg attgtcgacc tgggaccgga aggcggcagt ggcggcggcg
    53641 aaatcctcgt ctccggtacg ccagaaaccg tcgcagagtg cgaagcttcg catacggcgc
    53701 gcttcctcaa gccgatgctg taatcgttaa ggccgctttc agagcggcct tcttctttca
    53761 gagttgcacc agcaatttac gtttttcttc cggcagcaaa ttcaccgcct gctgataaga
    53821 cgcatccacc agataataga tttgcgaatc cggcagcgaa ccgtcgagat acacggtgct
    53881 ccagtgcgct ttattcagat ggcggcttgg acgcacatcg ctgtgctgct gacgtagcag
    53941 ctccgccagc tccgggctgg ttttcagcga aacagctggg cgattttcta cttctttcac
    54001 catcgcaaac agcacatctt ccactttgat ctgcgtcgct ttccagtcgt tatgcacgct
    54061 ctgttctgcg cctggttttg ccatgcaata ttgtagcaac tccgaaatgg tcatttttca
    54121 ctccccttgt agtgtcgcga tgatgcgacg cgatccgccg tggatgcgat gttcccccag
    54181 ccagatgcct tgccaggtgc cggtctgaat acgcccttta tgcaccggca atacaagcga
    54241 tgttcccagc attgaggatt tgatatgaga aggcatatcg tctgctccct catagtcatg
    54301 ctcataattt ccgttatcgg gaacggcgcg gaggaaaaaa cgctccatgt cgtggcgtac
    54361 ggtgggatcg cagttctcat taagtgtcag agaggcggag gtatgttgca gcaacagatg
    54421 cagtaaaccg atgttaacgc gcggcatatc agccagctga ttcagaattt catccgttac
    54481 cagatgaaac ccacgagatt tggcgctaag cgtgagcgtc ttttgatacc acatgtgctg
    54541 ctccttgata aaactctctt aatcagtttg cagcaagaca gcgaaaggat aaaggtgtga
    54601 ttaaaaaaac agcattcggg agagtattac gctctcccgc tctgtcagta ttctgaattg
    54661 acgatcacct cttcaccaaa cgcacccgct tgtggcaagg gtttgtaggt agagttggag
    54721 gcgcgcagaa tgcggatacc acgagcgccg acatcgcgtg cggcggtaat atcgttatcg
    54781 gaatcaccat aaaaaattcg gatattttta tcctgcagcc attgcgattt tgtattttgc
    54841 cctggtttat cgcccgcaaa gatcaccgga ttcatgttgg tggcaggaat atgaaaatta
    54901 tccgccagcg tttttgaaac cgtttctgtt ttggtcgggc tacgaccagt cacaaagaag
    54961 atcgcgtcac cgcggcgtac atgcatatca atcagctggc gagcgacctc ttttggaatg
    55021 ctgaattcat cccagccatt gttcattttt tcccagaaca caggattttt cagataatct
    55081 tcgctttctg gcgagaaggt ttttttgccg cgccagaagc ccggactgga aaaaagtacc
    55141 gtgtcatcga tatcaaaccc caccgccatt ggcggacgcc ctgcgaggct attttcaatt
    55201 tgtgcgaccg aaacccaatg aattggtgcc tgttcagcaa gcctggcaac gttagtacca
    55261 gggttaagcg gtgaaggaga tgaggccagg gcaacagcgg aactgtttag agcgaacaat
    55321 aagcaaacgg cactgattgc ctgtgtgatc ttgcgcatat ttttccctaa atagtcagta
    55381 tgttgaaact ttttattgtg agatttgttg caaaaaccat ctgaccataa cgccagcagc
    55441 cggggaaagg aaggattatc tgcggttttt gtgatgaaaa gcaggatgat tatatttaac
    55501 ccagtaaata cgttcctgtc gttttttgta aaaaaaatgt ttattacgcg atgtaagaat
    55561 acattaattt tgcgtttatt atgatgaatc ctctgcaaat gagcgaaatt tgatagaaca
    55621 tttatctgct gtacgattta ataaacttaa tgatacagca ttacaaaaca atcgaagttt
    55681 ataaagatga tttctgattg accagccccc taccctacaa tggtgactaa ttttattact
    55741 cccgaaggag atgatgatat gaatatatca aatgtgaata gcaacaacac gacatcttta
    55801 cctgtagagc tagatacact aaataacaaa ggtatctctt atgccaaaga tttctcttat
    55861 gccaaagatc tctttttata tatagaaaca cagttaaaaa ttgcaaaaga tttttgtaga
    55921 cctggagaag aagtatcaag ttctattgca agtacagttt ttcacgcatt tattgattta
    55981 gttaacaaaa tcaggggtaa gaaagattgt atgtatattt gcacgctttg ctgttttgcc
    56041 gaggaggtta aaggtgatta ttctcattac aggacctttt tatttgatat tggtaatcaa
    56101 tataaggtta aacttacaca aagcggaaaa aaagagttct ctttaacttt agaatttaac
    56161 gatactataa ttgaatctca gatagtcaca ggcaataagg caaagcatat tcttgaggat
    56221 atagaaaaat tctatcgtaa caaacccgat acttattatt aaatttaata atggttagat
    56281 gtgcaaaaat tattattcca cacggtaaaa cagggaaaag tactccagaa cattgggata
    56341 aacttaacaa gttagaacta acaacgtttt attacctctc acgtagaacg atggcatcaa
    56401 aaaaatcatc tataaagcaa gagtgaaagc gacgacagtt taatttcact gcaggctgga
    56461 taactccagc ctgctttcct gcattacatc accgcagcaa acgcctttgc cacacgttgt
    56521 acatttgccg tatttaaccc ggcgacacac atgcgaccgc tggcgatgag atagacacca
    56581 aattcttcac gtagtcggtc aacctgagcg gcacttaaac cggtataact gaacataccg
    56641 cgctgattaa gcagataatc gaaattgcgt tctggtatct ctgtgctcaa taccttcacc
    56701 agttcctgac gcatcgccag aatgcgagta cgcatctctt ctacttccgc cagccagctg
    56761 gctttcaatg cctcgtcatt cagcaccgca gccaccacct gcgcaccaaa attcggcggg
    56821 ctggagtagt tgcggcgaac tgttgctttc aattgcccca gtacgcggcc tgcggcttcg
    56881 gcatcttcac acagaacaga aagtccgccg acgcgctcgc cgtaaaggga gaaaattttc
    56941 gagaacgaat tgctcaccag agcgggtaat ccagcgctgg caatggcgcg aatggcgtag
    57001 gcatcctctt ccataccggc accaaatcct tgataggcaa tatcgaggaa tggaataagc
    57061 tcgcgggctt tgagaatttc aatcacggaa tcccactggt cattagtgag atcggcaccc
    57121 gttgggttgt ggcaacatgg atgcagcaac acaatactgc gggcaggtaa tgttttcagc
    57181 gtcgccaaca ggtcattaaa gcgcacgccg ttagtcgctt cgtcatacca ggggtaagtg
    57241 ctcacttcga atccagcccc ggcgaatatt gctacgtggt tttcccaggt aggatcgctg
    57301 acccagacgc ctgattccgg gaagtagcgt ttcaggaaat ctgcacccac tttcaatgcc
    57361 cctgagccgc caagggtttg aatggttgct acgcgctgtt gttgcagtac cggatggtcc
    57421 gcaccaaaca gcagcggcgc aatggcatgg cgatagctgt taagcccttc catcggtaaa
    57481 taaagcgaag caccatgagg ctgcgcattc aggcgcgctt ccgcatccgc tacggctttc
    57541 agttgtggaa taattccgtc ttcgttgtag tacagaccga tacttaaatt cactttgtcg
    57601 ctgcgagggt cttctttaaa acgctccata agcgtaagaa tcgggtcgcc agcgtaggcg
    57661 tcaacttttt gaaacacgcg atggttctcc aggtttacgg gcaggtggtt aaaacacaat
    57721 aaaccggaag aaggcgaaga tcgagaggat gttcaggggc gaatggcaat tagcaacaga
    57781 gtgagactca tgacaaacgt acatccgcca gatgcacgac ctttataaga gcatgaaaga
    57841 atatcaactt attgaattgg taagatttta ttggccggat aaggcattca cgccgcatcc
    57901 ggcacagaca atcaaatatt acagaacgat taatccacgt atttcatcgc gacccttgaa
    57961 gtcaggcgcg taataagttc gtaagcgctc acttttgtca tttcagcgat acgttctacg
    58021 ggcaaacctt cgccccataa aatgaccggg tccccggctt tgtcctgcgc ctgtggacct
    58081 aagtctacgc agatcatatc catcgcgacg cgcccgacaa tcggcacttc gcgaccgttc
    58141 accagcactg gcgtaccgga cggcgcggcg cgcggataac catcgccata gcccatcgcg
    58201 actacgccaa ggcgggtatc acgttcgctt acccaggttc caccataacc gacaggctct
    58261 ccggctttat gctcacgcac agcaatcagg ctggaggtca atgacattac cggctgacag
    58321 ccaaaatcgg caccggtgga acgatcttcc agcggcgaga cgccataaag aatgatgccc
    58381 gggcgcaccc agtcaaaatg cgactgtggc cacagcagaa tgccacccga cgcggcaatg
    58441 gaacgttgac ctggtttgcc ttcacaaaag gtattaaaga tagcgagttg tttctcggtt
    58501 gcgccgcatt ttggttcatc cgcgcgcgca aaatggctga cgatattcac cggctgacga
    58561 acgtttttgc actgggtcag gcgatgataa aacgcctcag cctgttccgg ccttacgccc
    58621 agacggtgca taccggtatc gagtttcatc cagacggtaa ccggctcgtc caggctagcc
    58681 tcttccagcg cagccagctg ttcttcgtta tgcacggcgg tatgaaaatg ttgcgcagaa
    58741 atcgtcggca gatctctggc atcaaaaaag ccttcgagta acagtacagg tttggtgatt
    58801 ccccccgcac gcagtcgcag agcttcttcg agacgggcta cgccaaaggc gtcagcatcg
    58861 gggagcgttc gcgcggtctc aagaagaccg tgaccataag cgttcgcttt caccaccgca
    58921 accattttac tggcaggagc cagttcacga agacgttgca ggttgtgtcg cagagcgcgg
    58981 cggttaatca caacagttgc cgcttgcatt tgtgttcctt gataagtgtt tgctttaatt
    59041 acctaattca taaaataatt attattcgtc gtcgtactgc ggccccgcat agttgtcgaa
    59101 gcgcgaccat tgaccgttaa aggtcaggcg taccgtcccg attgggccgt tacgttgttt
    59161 accgataata atttccgcga tgccttttaa atcactgttt tcgtgataca cctcatcacg
    59221 atagataaac atgatcaagt ccgcatcctg ctcgatagag ccagattcac gcaggtcgga
    59281 gttgaccggg cgtttgtcgg cacgttgttc cagagaacgg ttcaactggg acagcgccac
    59341 caccggcacg ttcaattctt tcgccagtgc tttcagcgag cgggagattt ctgcaatttc
    59401 cagcgtacgg ttatcggaaa gcgccggtac gcgcatcagt tgcaggtagt cgatcatgat
    59461 aagcccgatg ccgccgtgtt cacgggcaat acggcgcgcg cgggaacgca cttccgttgg
    59521 cgtcaggccg gaggagtcat cgatatagat attgcgtttt tcgagcaaaa tacccatggt
    59581 gccggaaatg cgcgcccagt cttcatcatc gagctgcccg gtacggattt tagtctggtc
    59641 aacgcgcgac agcgacgcca gagaacgcat cataatctgt tctgatggca tctccagcga
    59701 gaagataagt actggtttat cctgcaacat cgccgcgttt tcgacgaggt tcatcgcaaa
    59761 tgttgtttta cccatcgacg gacgcgcggc gacgatgatc aaatccgacg gctgcaagcc
    59821 agcggttttt ttgttgagat cgtcataacc ggtgtttacc ccggtaacgc catcgtgtgg
    59881 ctgctgaaac aactgctcaa tacgcgccac cgtggcgtcg agcacatcag cgatgttctt
    59941 cggcccttcg tctttgtttg cacgactttc ggcaatttta aagacgcggg attcagccag
    60001 gtccagcaga tcttcgctgg tgcgcccctg cggatcaaaa ccggcttcgg caatctcatt
    60061 cgcaaccgaa atcatctcgc gaacaacggc acgttcacgc acgatgtcag cataagcact
    60121 gatgttcgcc gcacttggcg tattttttga cagctctgcc agataagcaa aaccaccgac
    60181 gctatcgagt tgcccctggc gttccagcga ttccgcaagg gtaatcagat cgataggact
    60241 accgctttcc tgcaaacgcg ccatttcagt aaagatatga cggtgtgggc gggtgtaaaa
    60301 atcatctgcc accacacgct cagctacatc atcccagcgt tcgttatcca gcattaaacc
    60361 gcccaacacc gactgctccg cttcgatcga gtgcggaggc actttcagcc cggcaacttg
    60421 tggatcgcgt tcgcggggtt cagcctgctg tttgttgaag ggtttatttc ctgccatagt
    60481 gaatggagtt accgagataa agaatgggtc gaaactttac catatgaagc agaccctgac
    60541 gatacgttct ggaggacaca tggcaacacg aattgaattt cacaagcacg gtggcccgga
    60601 agtacttcaa gccgtagagt tcactcctgc cgatccggcg gagaatgaaa tccaggtcga
    60661 aaataaagcc atcggcatca attttatcga cacgtatatc cgcagcggcc tttacccgcc
    60721 gccatcgcta cccagcggat taggcaccga agcggcaggc atcgtgagta aagtcggcag
    60781 tggtgtaaag catattaagg caggcgatcg cgtagtctat gcacaatcgg cattaggcgc
    60841 ttacagctct gtgcataaca ttaatgcgga taaagcggcg attctgcctg cggcaatttc
    60901 ttttgagcaa gctgcggcat ccttcctgaa aggcttaacg gtttattatc tgctgcgcaa
    60961 aacctatgaa attaaacccg atgagcagtt cctgttccac gcagcggctg gcggcgttgg
    61021 cttgattgcc tgccagtggg caaaagccct tggcgcgaaa cttatcggca ccgtaggaac
    61081 cgcgcaaaaa gcgcagagtg cgctaaaagc gggcgcatgg caggttatta actatcgtga
    61141 agagaatctg gtcgagcggt taaaagagat caccggtggc aagaaagtgc gcgtggtgta
    61201 cgattccgtg ggcagagaca cctgggaacg gtcgctggat tgcctgcaac gccgcggctt
    61261 aatggtgagc tttggcaact catcaggtgc ggttaccggt gtgaacttag gcattctcaa
    61321 tcagaaaggc tcgttgtatg tgacacgccc ttccctacaa ggctatatca ccacacggga
    61381 ggaattaacc gaggccagta atgaactgtt ctctttgatt gccagcggtg tgattaaggt
    61441 cgatgtcgcc gagcagcaga aatatccgct taaggatgca cagcgtgcgc atgagattct
    61501 ggaaagccgg gcgacgcaag gttccagcct gttgattcca taaaagaaat agggcttcca
    61561 tctgggaagc cctttctttt tatagttcgg ctgtatgtag ggtacagcac gatgaatctg
    61621 ttagaggcgc aatagtgaca gatttgatta tcaattccta ttttgttcta aggataaaac
    61681 cttaggttgt gatcatccgc acaatccctt agtaacgcca gcggtcataa cgctgatatt
    61741 tcggcacttt tggtgcttta atcgccttaa taacccacac caccgcaatc gccaggagta
    61801 accacggcag caacttaatc atcaatgcca gcataccgcc gaggaacata atggccgtcg
    61861 ccacaaccag cgcggcgata atgcccagca acgaaacgcc ggtgaccatc agcatgacaa
    61921 aaaagccaat cacaaaaagt agttccagca tgatgctctc ccaaatatga aatctcttgc
    61981 aggcattaca agaatcatgc caaaaataat ctattgattt aacagcaaaa cgccccgcga
    62041 cggtgcgcag ggcgtggtga atttgactac tttttggtga aaagttaacg cttatccgcc
    62101 accagtttga gcgcgtgttc cagcacgtta atgtcagcac ccgctttatg ggcattttca
    62161 cttaaataac gccgccactg ccgcgcgcca ggaataccct ggaacaagcc cagcatatgc
    62221 cgggtaatat ggccgagata cgtcccctgg ctgagttcac gctcaatgta cggatacatg
    62281 gcacgcacca ccgccaccgg atcgacatcg gtatccgaag aaccaaagat ctctcggtct
    62341 accgccgcca gaatgcctgg attctgatac gcttcgcgcc cgaccatcac gccatccata
    62401 tgttgcaaat gcgctttagc ctcttccagc gacttgatgc caccgttaat cgacatcgtc
    62461 agatgcggaa agtcacgctt cagttgatac acacgcggat aatcgagcgg cgggatttca
    62521 cggttttctt tcgggcttaa ccccgaaagc caggctttac gtgcgtggat gatgaacatc
    62581 tcacactcac ctttgccgga aacggtgttg atgaaatcgc agagaaattc atagctgtcc
    62641 tgatcatcga tgccaatacg cgttttcacc gtcaccggaa tcgacaccac atcgcgcatc
    62701 gctttcacgc agtcggcaac cagctgcgca ttacccatca gacacgcacc aaacatgccg
    62761 ttctgcaccc ggtcagacgg gcagccgaca ttcaggttga tctcatcata tccgcgcgct
    62821 tctgccagct tcgcacactg tgccagcgcc gccggatcgc tacccccgag ttgcaacgct
    62881 accggatgtt cttcttcact gtacgccagg taatcacctt taccgtgaat aatcgcccct
    62941 gtagtcacca tttcggtata cagcaacgta ttgcgggaaa gcagacgcaa gaaatagcgg
    63001 caatgtctgt ccgtccagtc aagcatcggg gcaatcgaga aacggtaagc gggataaaca
    63061 gtgtgtgatt ctgctggcat ggcgactttt taagcatctg gtaaattggg ggcgctacta
    63121 tagcataacg aatatgcgat caggcaatgt gacagccatg acaccctgtt ctcggcgttt
    63181 aagcgagaac tatggtaaag taaggacatt cttaacccca ctgtcgaggt gcccaatgga
    63241 aaagaccaca acgcaggagt tattagcgca ggctgaaaaa atctgcgcac agcgtaatgt
    63301 gcgcctgacc ccacagcgcc tggaagtatt gcgcctgatg agtctgcagg atggcgctat
    63361 cagcgcttat gatctgcttg atttgctgcg cgaagctgaa ccgcaagcca agccgccaac
    63421 ggtttatcgc gcgctggagt ttctgcttga acaaggtttt gtgcataagg tggaatccac
    63481 caacagttat gtgctctgtc atctgttcga tcagcccacc catacttcag ccatgtttat
    63541 ttgcgatcgc tgcggcgcgg tgaaagaaga atgtgctgaa ggcgtggaag acatcatgca
    63601 tacgctggcg gcaaaaatgg gttttgccct gcggcataat gttattgaag cacatggatt
    63661 atgttcagca tgtgtagaag tagaagcgtg tcgtcatccc gaacagtgcc atcatgacca
    63721 ctctattcag gtgaaaaaga aacctcgcta aaagggtgta catccttgta catgtcgggc
    63781 aggagggatt aattaccagc ggtaatcatg gcgtttttcc caactatcaa cctctttttc
    63841 tgcctgatct ttctgataac cataacgttc ctggatttta ccgaccagtt gatcacgttt
    63901 accttcaatg atcgtcatat catcatcggt cagtttgccc cattgctctt tcactttacc
    63961 tttaaactgt ttccagttac cgccggcttc atctttattc ataatcaaga cctcatcgtt
    64021 aggttgtgaa tgagagtacg ttcacttttc ttctgaacgt gagattaagt gtagtcagca
    64081 atttggctta ggattattta ttcagaattt ttaaccgtca cgttgcggca aaccaggtac
    64141 cgttgcgcca gtgacgccgc cagatagccg ccagagaaag cccgcgcaaa gccagaaaga
    64201 cggttaatgc cagccacaaa ccatgattcc ccagccacgg cagcgtaagg agcgtcagcg
    64261 caaaacctgc ggcggccacc gccatactgt tacgcatttc ggcggcgcgc gttgcaccta
    64321 taaacatgcc gtcgagcaga taacaccaga cgccgaccaa cggcaaaatc acctgccaga
    64381 taagatagcg gtcagccagc tgctgaatct gggttaacga cgtcagcaac gcaatgatgt
    64441 gttcccctgc cagcaaataa accaccgaaa acagtaacgc tacgatcccc gactggcggc
    64501 acgctgcccg ccagacatcc agtaactggc taccatcgcg cgcaccgtac gcctgaccgg
    64561 aatgcgcttc aaccgcgtag gcaaaaccat ccagcgcata ggcggtaaag gtgagtaacg
    64621 tcatcagaac cgcgttaaca gcgataatgt cactccccag tcgcgcgcca agtacggtga
    64681 tcgcgccgaa acagagttgc aacaacagcg agcgcagcat gatatcgcgg ttaagcgcca
    64741 gcaagtgacg gaagtttcct cgccaggcag ttttcagcat ttcgccggag attccgcgta
    64801 gtttgaggat tttacgcacc attagcagac caatcagcaa tgttgcatat tccgcaataa
    64861 ccgtcgccag cgccgcgccc tgcacgttca tatgcagccc catcaccagc cagacatcca
    64921 gcacaatgtt gaggatattg ccgaccacta acaaaattac tggcgcacgg gcatattgca
    64981 caccgagtaa ccaaccaagt aataccagat tcgccagcga cgccggtgca cttaaccagc
    65041 ggatttcaag aaagcgtcgc gcctgttcta gcaccgcttc gctgccgcca acaatatgca
    65101 gcgccagatc gataatcggc gtacgcagca gcgcaattaa cgccccagcc cccaacgcca
    65161 acagcaacgg ttgcaccagc gcacgggcta atgcctgagg atttttggca ccataagcct
    65221 gcgcagtcag cccggtggtg ctcatgcgta aaaacagcaa cagcataaag agaaagctgg
    65281 tcgccgttgc accaaccgcc acgccgccca aataaaccgg actatcaaga tgaccaatta
    65341 ccgccgtatc gaccagtccc agcaacggaa cggtgatatt ggagaaaatc atgggtaagg
    65401 cgagatgcca gagtgcttta tcagatgaag tgaggaatgc catgcagaca agcctgatga
    65461 agagagatga aaaacaaacc gcgacgccag gcggcatcgc ggtctcagag atatgttaca
    65521 gccagtcgcc gttgcgaata actccaaccg ccagcccttc aatggtgaag ctctgctgac
    65581 gaaggtcaac gacaattggt ttaaactcgc tattttctgg caacagttcg actttattgc
    65641 cctgtttttt caggcgctta acggtaacct cgtcatcaat acgtgcgaca acgacctgac
    65701 cgttacgtac atcctgagtt ttatgcactg ccagcaagtc gccatccata atgccgatat
    65761 ctttcatcga catcccgctg acgcgcagca ggaaatcagc attcggcttg aataaggaag
    65821 gatcgacctg ataatgacct tcaatatgct gttgcgccag aagcggttca ccggcagcca
    65881 cacgacctac cagcggcaac ccttcttcct cttcctgcaa cagacgaatc ccgcgtgatg
    65941 cgccggaaac aatttcaata acgcctttgc gtgccagcgc cttcagatgt tcttcagccg
    66001 cgtttgggga acggaacccc aaacgctgcg cgatttccgc acgcgtcggc ggcatacctg
    66061 tctggctgat gtgatcacgg atgagatcaa acacctcttg ttgcctggcc gttaacgctt
    66121 tcattccgcc ccctgggtgt atatacagtt atgctgtgag tatatacagc aaaaggcgat
    66181 tttggaacca taaactgcac aataaaccag agatttatcg aaatctggaa gggttatcca
    66241 aaatgcgacc ataacaggat gcaccaggta atcacggcga cgataatcgc aatcagaacc
    66301 gcggcggatc ccatatcttt agcgcgtccg gaaagctcat ggtattcaga gccaattcgg
    66361 tcaaccactg cttcgatggc gctattgagg atttccacaa tcatcaccag catcacggag
    66421 ctgataagca gcacgcgggt aattgcgtcc acatccagcc agcaggcgat gaccaccgcc
    66481 aacaataccg ctacgccttc ctgacggaat gccgcttcgt tgatccatgc agcgcgtaaa
    66541 cctttccagg aatagccagc agctttgata attcgggtga atccagtggt attattggcc
    66601 attgaaagaa cctttttaca ttatgagcgt caatatcagt gtaccgttaa atccgatgat
    66661 tcgggtttgc gtaacgccgc gcgaaacatg agcggatacc acagaatttc ccatgacttt
    66721 ctgctatcct tgccgcgcat ttgcattatt aaccagaggc tttacatcgt ttatgtccgg
    66781 ctggccacga atttactaca aattactgaa tttaccatta agcatcctgg taaaaagcaa
    66841 gtctattccg gcagatcctg ccccggaact ggggctggat acctctcgtc caattatgta
    66901 cgttttaccg tacaactcga aagctgactt gctgacgttg cgcgcccagt gtctggcaca
    66961 tgacctgcct gacccgttag agccgctgga aatcgacggc acgctactgc cgcgctatgt
    67021 gttcattcac ggcgggccgc gtgtgttcac ctattacacg ccgaaagaag agtctattaa
    67081 gctgttccac gactatctcg atttgcaccg tagcaaccca aatctggatg tgcagatggt
    67141 gccagtatcg gtgatgtttg gtcgcgcgcc ggggcgtgaa aaaggcgaag tgaacccgcc
    67201 gctgcgtatg cttaacggcg tacagaaatt tttcgctgta ctgtggctcg gtcgcgacag
    67261 ttttgtgcgt ttctcgccgt cagtttcgct gcgccgtatg gcggatgaac acggcacgga
    67321 taaaactatc gcgcagaaac tggcgcgcgt ggcgcgtatg cactttgccc gtcaacgtct
    67381 ggctgccgta ggcccacgtc tgcctgctcg tcaggatctg tttaataagc tgctcgcctc
    67441 ccgcgccatt gccaaagcgg tagaagatga agcgcgcagc aaaaaaatct cccatgaaaa
    67501 agcgcagcag aacgcgattg cgctgatgga agagattgcg gcgaatttct cttacgagat
    67561 gatccgcctg actgaccgta ttctgggctt cacctggaac cgcctttacc agggcatcaa
    67621 cgtccataac gccgagcgcg ttcgtcagct ggcccacgac ggtcatgagc tggtgtatgt
    67681 gccttgccac cgcagccaca tggactacct gctgctttct tacgtgctgt atcaccaggg
    67741 gctggtgccg ccgcatatcg ccgccgggat caacctgaat ttctggcctg ccgggccgat
    67801 tttccgccgt ctgggggcgt tctttattcg ccgtacgttt aaaggcaata agctctattc
    67861 caccgttttc cgtgagtatc tcggcgaact gttcagccgt ggttattccg tcgagtattt
    67921 cgtggaaggc ggtcgttccc gtacagggcg tttgctggat ccgaaaaccg gtacgctgtc
    67981 gatgaccatt caggcgatgc tgcgtggcgg cacgcgtccg attacgctga ttccgatcta
    68041 tatcggttat gagcacgtca tggaagtggg tacttacgcc aaagaactgc gcggtgcgac
    68101 gaaagagaaa gagagcctgc cgcagatgct gcgcggttta agcaagctgc gtaatctcgg
    68161 tcagggttac gtcaacttcg gtgaaccaat gccgttgatg acctacctta accagcatgt
    68221 acctgactgg cgtgaatcta tcgatcccat cgaagcggtg cgtccggcat ggttaacgcc
    68281 gacggtcaat aatattgctg ccgatctgat ggtacgcatt aacaacgcag gcgcggcaaa
    68341 cgccatgaac ctgtgttgta ctgcgcttct ggcgtcacgt cagcgctcac tcacccgcga
    68401 gcagttaacc gagcaactca actgctacct ggatctgatg cgcaacgtgc cttactccac
    68461 ggactctacc gttccttcag ccagcgccag cgagcttatc gatcacgcgc tgcaaatgaa
    68521 caagtttgaa gtcgagaaag acacgatcgg cgacatcatc attctgccgc gcgagcaagc
    68581 ggtgctgatg acctactatc gcaacaacat tgcgcatatg ttggtgctgc cttcgctgat
    68641 ggcggcaatt gtcacccagc atcgccacat ctcccgcgac gtattgatgg agcacgtcaa
    68701 tgtgctttac ccaatgctga aagcggagct gttcctgcgc tgggatcgcg acgagttgcc
    68761 gggcgttatt gatgcactgg caaatgagat gcaacgtcag gggctgatta ccctgcaaga
    68821 tgatgagttg catatcaacc cggcgcattc tcgcacgcta cagctgctgg ccgcaggcgc
    68881 gcgcgaaacg ctgcaacgtt atgccatcac cttctggctg ttgagcgcca acccgtcgat
    68941 caaccgcggt acgctggaga aagagagccg caccgtcgcg caacgtctct ccgtgctgca
    69001 cggcatcaac gcgccggagt tcttcgacaa ggcggtgttc agttctctgg tgctgacact
    69061 gcgtgatgaa gggtatatca gcgatagcgg cgacgccgaa ccggcagaaa cgatgaaggt
    69121 gtatcagttg ctggcggagt tgattacatc agacgtgcgt ttgacgattg agagtgcgac
    69181 gcagggcgaa gggtaatcag ggagaattgc cggatgcggc gcaaacgcct tattcggcct
    69241 accatgatct gcaaattcaa taaattgcga tatgttgtgt aggcctgaga agcgcagcgc
    69301 atcaggcaac ccagaaggaa gccggatgat catccggctt ttttacatca tcagaaatgc
    69361 cagtaactca ttgccagccc taaaaacagt accagaccaa catagttatt attcataaat
    69421 gctttaaagc aggcttcacg ctcgcggttg gcaatcagtt tttgttgata aacaaacagc
    69481 gcgccagcca ccagaattga ccagtaatat ccccagccta agccatttaa ctcaccgata
    69541 atcgccatca gcgccagtac gccaatctgc aaaataccaa taatcaattt atcgtattgg
    69601 ccgaacagga ttgcggtgga tttaatgcca atcttcacat catcatcacg gtcaaccatc
    69661 gcatactgcg tgtcgtatgc caccgcccag agaatattgg cgaggaacat taaccagcaa
    69721 ctcaatggca ccgactcact cacagcagca aaagccattg gaatcgacca gccaaacgcc
    69781 gcgcccagca ccacttgcgg tagatgggta taccgcttca taaacgggta tacccacgcc
    69841 agcgccagcg cggcaatcga caacagaata gtcatcgtat tcagcgtcag caccagtaaa
    69901 aatgaaatca gtaccagcac tacaaacagc gcgcgcgcct ctttctccgt taccgcgccg
    69961 ctggggagtg gtcggttcgc cgtgcgctta acatgaccat caaacttgcg gtcagcataa
    70021 tcattcacca cgcaaccagc agcgcgcatc agccagacgc ccgcgacaaa caccgccagg
    70081 atccagagct ggggaacgcc cggtgtcgcc acccacaacg cccataatgt tggccagagc
    70141 agcagtaacg cgccaattgg cttatccgta cgcattaagc gatgaaacgc cagcagctta
    70201 ttctgcgtca gactccactc catatttttt tcctcttagt acaacggtga cgccggtaaa
    70261 aacagttctg ttagcaacag cggtttaccg cttaatcgca ggcgggaacg tcgcccccac
    70321 agtccggcat cacggcctat ctcaataaag tcccgggtta atgtcgatga tgtgaacaga
    70381 tagcgtccta acggcgtttt acctaatttt tgtaacgcca gctccggccc gcttaacgtt
    70441 gacacaggaa cgacggtacg accggcaagc cacggttcac catcggcaca taacaaaatt
    70501 tcacgtaacc agtaacgaga ctctttcggc agcagtggca gttcttcggg gatttcattc
    70561 tgctcgacaa acccttcgcg gatcatcgtc acgctcaccg tttttccctg ctgttcaaaa
    70621 cgttttgtca tggaatcttc cagcaacagc cagtcgagca gttgcggctc cagggcaggg
    70681 atctctgtaa aatagcgcag cgcacgcagt tgcgttaacg cggggtgtga catgccgaac
    70741 tctccgttac ataacgtaaa ggcattgtat cgcagaaaaa tatttacggg agatgtaaag
    70801 caaattaagt gaactaatgc gcaacaaaaa cgcaacaata gtacaggagg agaaaaaaag
    70861 gtgcgccagg agacgcacca gttgttgcaa aatcagcact gtggggcgta attacccctt
    70921 gccttttaca ctgctgataa aggtggaacg ggcagatgtc gaccccaggc gttcagcttc
    70981 atcaagcagt tttaacgcct tatcaacatc acctttcgcg acagcgtttt tgatcgcggt
    71041 attaaaataa ctttccgtgt cgttgagcat cggctcgctt ttcttcaccg gtgccggagc
    71101 gggtgcagcc acagctggtg ccgccgtgtt gcctaccgta accggagctg gagcggaaga
    71161 accaaataga ggcccgacca acacgctaga actggagttc gttttgactt tcagtttcag
    71221 taagccatcg gtggtatgac gagcaaccgg gtcaggaata tccgggatcg agttaccgac
    71281 gcctttggca taggctttag ccgggtcgag cagttgggtc gtctgctgga ggtctttttc
    71341 cgtggtaaag accagaacat aaagtttttg ctgccccaac gccggtgtca ggcgcataac
    71401 gccttccaga cgatctgcac tcatcacgcc tggttcctgg taggtgaaat aactgctggg
    71461 gaagaaggct gatggggtca tgttctgatc aagaatcagc acgttcggcg caaatacgct
    71521 agtttgtttg ttcacttcgc tggtcagcgt tagggtcagt tcgccaatgt ttgccgggac
    71581 gctgtacgca gcaaccggac ctctgatgcc gggaacgttc agttgttggc cgccagtcgc
    71641 cagttgggtg gtctgggttt tagattgatc gaccggtgtc caggtgagtt gttgcagcgc
    71701 ggcagatgga atggctggcg cgtcgctggt gttttgcggt acgtagttaa catcggcaag
    71761 gctgattcca ggtgcgcttg ccagcaaccc tgctgataaa cagagagcga tgagactttt
    71821 attcattttc attgttttca cctcaaaatc tggagctcag cggtagccag gcaatagcgc
    71881 gctaaacccg ataatcagag gggctttcgc cccttaaagc tgtttttatg ccggatgcgg
    71941 cgtaaacgcc ttatccggcc tactttcggc agcggttgta gttgtaggcc tgataagacg
    72001 cgcaagcgtc gcatcaggcg ttggttgccg gatgcggcgc aagcacctta tccggcccag
    72061 gttttgctat taccaccaga tttccatctg ggcaccgaag gtccactcgt cgctgtcacc
    72121 acggccgaag ctgccgccgt tgaaatcagc aggaacggct ttgccgaagt tagtgttggt
    72181 attggcgtta ccggtgtagt cataacccca tttctcatcc cacttggcgt aggttgcgaa
    72241 gacacgaata gccgggcgtg accagatgct gtcgccagcc tgccattgtt gtgcaagggt
    72301 aattttgtac tgattgttct tgtcgccggt gcgctgggat tcgacgttgt cgtagccgat
    72361 ttccatcacg gtgctcatga ttggcgtcca cttgtacatc gggcgaatac cgacggtcca
    72421 ccacttggtg ccgttgtcgt tatcccagtt gatatcctgg tacataccca cgtacatcat
    72481 gtcccagttg tcgcccatgg agatcgcacc gtggtcgagg atacgcagca tgtgaccgtt
    72541 gttgttgata ttgtaggcaa atttttcgtt atcaaacgcg acgccagaac cctgcgacag
    72601 acctttaccc tgcgaggtca tcgagtcagt agcgtactga acaacaaact tgttaaagcc
    72661 cttcaggaca ctctgagtat gttcagcagt gaataaccaa ccgtctttcg atgcgccatc
    72721 aaccagacga tagttatcac gcaggttggc acgaccgtag tcgacaccca gttctaatgt
    72781 gccgcccggg ttgatttcca tctgcgctaa acgcacgtcg aaaacgtcgt tcgcggtttc
    72841 gttggtatag tcataaatat tgttgctggc gaaagaggaa gaaccaccag cttcagagga
    72901 acgggttgct gccagagaga gtttaccgaa gccaacatcg atgttttcca gaccggcacc
    72961 aggaccagaa atatcccagt agtagaagtc gatcatatga acgtcatgac gttggtagaa
    73021 gcgcttacct gcccagatgg tggaacctgg cagccattcg atcaggtttt taccctgcac
    73081 gtttgcttca cggaaggccg gatcggtagc ttcccagtca ttctgttgtg cgacggaata
    73141 ggccacgtta gtgtcgaaat agaagctctt atcgccctct ttccacactt cctgacccaa
    73201 ttttaattca gcataagttt cacattcgtt gccaagacgg tatttacttt gagcaccggt
    73261 agtctggaaa cactgttgtt caccgccgct acctgtccag ccaataccgg aacgtgcata
    73321 gccgtggaaa tcaacagcca ttgcctgagc agacattacg cccgctgcga cggcaaccgc
    73381 cagaggaagt ttgcgcagag taatcatcat tctatctcct gagtcattgc ttttcttttt
    73441 tcacatcacc tgtgacaggc tttgtgtgtt ttgtggggtg cttaaacgcc cggctcctta
    73501 tgcagtcgac gacatgcagt gccatcctca cggaacagat ggcaacgctc tggcggcagg
    73561 ccgatagcga atgtggcacc ttcttctacc aacaccacgt cgttctggcg gtacaccagg
    73621 ttttgacgaa tggaagggat ctggatatgg atttgggttt cgttgccgag ttgctcgacg
    73681 acctgaactt caccctcaag gatgacgtca gcgatatcac tcggcagtag atgttccggg
    73741 cgaataccca gcgacatatt ggctccaacc tggacatcac ggctttcaac cggcagccag
    73801 acttgctgac gatttggcat cggcagctcc acctgcactt gatcgattgc agtggcggtc
    73861 acttttactg gcaggaagtt catctttggc gaaccgataa atccggcgac aaaacggtct
    73921 gccggatagt ggtacagctc cagcggtttc ccaacctgcg ccacgcgacc ggcgtccagc
    73981 accacgattt tgtcggctag tgtcatcgct tcgacctgat cgtgggtgac gtaaatcatt
    74041 gtgcggccca ggcgtttatg cagacgggag atttcgatac gcatttgcac acgcagtgca
    74101 gcatcgaggt tggagagcgg ttcatcgagc aaaaatacgc ttggctcggc taccagcgta
    74161 cggccaatcg ccacacgctg acgctgacca ccggagagcg ctttcggttt gcgatccagc
    74221 aaatgcgcca gttgcagcac ttccgcaacc tggttaactc gctggttaat cacttctttt
    74281 ttcgcgccag ccagtttcag gccaaatgac atgttttctg ctactgacag gtggggatag
    74341 agcgcgtaag actgaaacac cataccaacg ccgcgttctg caggcggagt gtcattcatc
    74401 cgtttctcac cgatgaacag gtcgccgctg gtgatcgtct caagcccggc aatcatgcgc
    74461 agtaaagtcg atttaccgca gccagacggt ccgacaaaca ccacgaattc accttcatgg
    74521 atatcgagat tgatatcttt cgataccacg acctcgcccc aggctttcgt tacattttgc
    74581 agctgtacgc tcgccatgcc cttctccctt tgtaacaacc tgtcatcgac agcaacattc
    74641 atgatgggct gactatgcgt catcaggaga tggcttaaat cctccacccc ctggcttttt
    74701 tatgggggag gaggcgggag gatgagaacg cagcttctgt gaactaaacc gaggtcatgt
    74761 aaggaatttc gtgatgttgc ttgcaaaaat catggcgatt ttatgtgcgc atctccacat
    74821 taccgccaat tctgtaacag agatcacaca aagcaacggt ggggcgtagg ggcaaggagg
    74881 atggaaagag gttgccgtat aaagaaacta gagtccgttt aggtgttttc acgagcactt
    74941 caccaacaag gaccatagat tatgaaaata aaaacaggtg cacgcatcct cgcattatcc
    75001 gcattaacga cgatgatgtt ttccgcctcg gctctcgcca aaatcgaaga aggtaaactg
    75061 gtaatctgga ttaacggcga taaaggctat aacggtctcg ctgaagtcgg taagaaattc
    75121 gagaaagata ccggaattaa agtcaccgtt gagcatccgg ataaactgga agagaaattc
    75181 cctcaggttg cggcaactgg cgatggccct gacattatct tctgggcgca cgaccgcttt
    75241 ggcggctacg ctcaatctgg cctgttggct gaaatcaccc cggacaaagc gttccaggac
    75301 aagctgtatc cgtttacctg ggatgccgta cgttacaacg gcaagctgat tgcttacccg
    75361 atcgctgtcg aagcgttatc actgatttat aacaaagatc tgctaccgaa cccgccaaaa
    75421 acctgggaag agatcccggc gctggataaa gaactgaaag cgaaaggtaa gagcgcgctg
    75481 atgttcaacc tgcaagaacc atacttcacc tggccgctaa ttgcggctga cgggggttat
    75541 gcgttcaagt atgaaaacgg caagtacgac attaaagacg taggcgtgga taacgctggc
    75601 gcgaaagcgg gtctaacctt cctggttgat ctgattaaaa acaaacacat gaatgcagac
    75661 accgattact ccatcgcaga agctgccttt aataaaggcg aaacagcgat gactatcaac
    75721 ggcccgtggg catggtccaa catcgacacc agtaaagtga attatggtgt aacggtactg
    75781 ccgaccttca aaggtcaacc atctaaaccg ttcgttggcg tgctgagcgc aggtattaac
    75841 gccgccagtc cgaacaaaga gctggcgaaa gagttcctcg aaaactatct gctgactgac
    75901 gaaggtctgg aagcggtcaa taaagacaaa ccgctgggtg cagtagcgct gaagtcctat
    75961 caggatgaac ttgcgaaaga tccacgtatt gccgccacca tggataacgc tcagaaaggt
    76021 gaaattatgc cgaacatccc gcagatgtcc gctttctggt atgccgtgcg tactgcggtg
    76081 atcaacgccg ccagcggtcg tcagactgtc gatgaagccc tgaaagacgc gcagactcgt
    76141 atcaccaagt aatgctgtga aatgccggat gcggcgtaaa cgccttatcc ggcctacgaa
    76201 attgaaacgt atgtaggcct gataagacgc gtcagcgtcg catcaggcag ttgttgtcgg
    76261 ataaggcgtg aaagccttat ccgtcctgga atgaggaaga accccatgga tgtcattaaa
    76321 aagaaacatt ggtggcaaag cgacgcgctg aaatggtcag tgttaggtct gctcggcctg
    76381 ctggtgggtt accttgttgt tttaatgtac gcacaagggg aatacctgtt cgccattacc
    76441 acgctgatat tgagttcagc ggggctgtat attttcgcca atcgtaaagc ctacgcctgg
    76501 cgctatgttt acccgggaat ggctggaatg ggattattcg tcctcttccc tctggtctgc
    76561 accatcgcca ttgccttcac caactacagc agcactaacc agctgacttt tgaacgtgca
    76621 caggaagtgt tgttagatcg ctcctggcaa gcaggcaaaa cctataactt tggtctttac
    76681 ccggcgggcg atgagtggca actggcgctc agcgacggcg aaaccggcaa aaattacctt
    76741 tccgacgctt ttaaatttgg cggcgagcaa aaactgcaac tgaaagaaac gaccgcccag
    76801 cccgaaggcg aacgcgcgaa tctacgcgtg attacccaga atcgtcaggc gctgagtgac
    76861 attaccgcca ttctgccgga tggcaacaaa gtgatgatga gctccctgcg ccagttttct
    76921 ggcacgcagc cgctctacac actcgacggt gacggcacgt tgacgaataa tcagagcggc
    76981 gtgaaatatc gtccgaataa ccaaattggc ttttaccagt ccattaccgc cgacggcaac
    77041 tggggcgatg aaaagctaag ccccggttac accgtgacca ctggctggaa aaactttacc
    77101 cgcgtcttca ccgacgaagg cattcagaaa ccgttcctcg ccattttcgt ctggaccgtg
    77161 gtgttctcgc tgatcactgt ctttttaacg gtggcggtcg gcatggttct ggcgtgtctg
    77221 gtgcagtggg aagcgttgcg cggcaaagcg gtctatcgcg tcctgctgat tctgccctac
    77281 gcggtgccat cgttcatttc aatcttgatt ttcaaagggt tgtttaacca gagcttcggt
    77341 gagatcaaca tgatgttgag cgcgctgttt ggcgtgaagc ccgcctggtt cagcgatccg
    77401 accaccgccc gaacgatgct gattatcgtc aatacctggc tgggttatcc gtacatgatg
    77461 atcctctgca tgggcttgct gaaagcgatt ccggacgatt tgtatgaagc ctcagcaatg
    77521 gatggcgcag gtccgttcca gaacttcttt aagattacgc tgccgctgct gattaaaccg
    77581 ctgacgccgc tgatgatcgc cagcttcgcc tttaacttta acaacttcgt gctgattcaa
    77641 ctgttaacca acggcggccc ggatcgtctt ggcacgacca cgccagccgg ttataccgac
    77701 ctgcttgtta actacaccta ccgcatcgct tttgaaggcg gcgggggtca ggacttcggt
    77761 ctggcggcag caattgccac gctgatcttc ctgctggtgg gtgcgctggc gatagtgaac
    77821 ctgaaagcca cgcgaatgaa gtttgattaa gggagataac aaaaatggca atggtccaac
    77881 cgaaatcgca aaaaacacgt ttgtttatta ctcacctgct actgctactt tttatcgcgg
    77941 cgattatgtt cccgctactg atggtcgtcg ctatctcact gcgccagggg aactttgcga
    78001 ccggcagcct gatcccggag caaatctcct gggatcactg gaaactggca ttaggtttta
    78061 gcgtggaaca ggctgatggt cgcgttacac cgccgccgtt tccggtactg ctatggctgt
    78121 ggaactcggt aaaggtcgcc gggatttccg cgattggcat tgtggcgctc tccaccacct
    78181 gcgcctacgc tttcgcccgt atgcgctttc caggcaaagc gacgctgctg aaaggaatgc
    78241 tgattttcca gatgttcccg gcagtacttt cactggtcgc gttgtatgcg ttgtttgatc
    78301 gtctgggtga gtacattcca ttcattggcc tgaatactca cggcggcgta attttcgcgt
    78361 atctgggcgg gattgcgctg catgtctgga ccatcaaagg ttatttcgaa accatcgaca
    78421 gttcgctgga agaagcggcg gcgctggatg gtgcgacacc gtggcaggcc ttccgccttg
    78481 tcctgttgcc gctgtcagta ccgattctgg cggttgtgtt catcctgtcg tttatcgctg
    78541 ccattactga agttccggtc gcgtcgctat tactgcgtga cgtaaacagc tacaccctgg
    78601 ctgtggggat gcagcaatac ctcaacccgc aaaactacct gtggggtgac tttgccgccg
    78661 ctgccgtgat gtctgcatta ccgatcacca tcgtcttctt gctggctcaa cgctggctgg
    78721 tcaacggcct gacggcaggt ggtgtgaaag gttaaagatg ttgttctgcc aatgttatgc
    78781 cgctgcaccc tcaacttacg ttatcccaac ttgtgactgt tattcggcgc tccacggagc
    78841 gccttttttt actcccgctt cagccgtttc gaattacaca accacagggt gatcaccagt
    78901 aacaggatcg ctgccgagta gatcaacaca tccagtggcg atttatgatc gacgatgatc
    78961 aagcgcacaa tggcggtgat cccaatatag acaaagtaac gtagcgggaa gtgaaaaccg
    79021 gactgaaagt acttcacaat cagcgcgata aattcgaaat agagaaagta aaccaccagc
    79081 ccttctacca gctcatattt actggtttgt tctggcgcga acagcacatc agccagatgc
    79141 accgtttctt tgccgaggaa gacaaccaaa atcaggccga ggcacaacag gccaagattg
    79201 agtacggtct gcaaaatggt ggatataaac tccacgcgcg gacgagagag tgacgtcata
    79261 gcagtacctc cttcacagtg gcgagcttcc tgtataacgc aaaaatgtga cggagatcta
    79321 tattttttgt gtatgttttg ttcagctcaa ttaagtgggc atctcccatc aatgtctcat
    79381 ctggcaaaac acacagccaa aaaaatcacc cattcaggtg attcccagcc accagcaatg
    79441 cgcgaaaaat cgacaatgtt cctctggaac aaatgaaacg taacctgata aaggtggtaa
    79501 aaatgaagcc ttattttgct gctttgatgt tatcagtctc tgttcttcct gcttatgctg
    79561 gtccgctcgg tactgctgat aaagccgacc taccgcaaag caatgtctcc agtccaatga
    79621 tggcccagtc gctcaggcaa ccagacctgc aaccgatatc gacggacagg aagacagaat
    79681 gttttcgcct ttacacacca gatcgcaaac cgggggtgaa ctgcgttcct gatggctcga
    79741 caggccatta agaatgaggg tgccagtgag tgcgaaaact ggcacctttt ccatgactta
    79801 gcggaaattg acgctacggt cgctggtcat gtcatacaac tggaacttac gcccaagttg
    79861 ctgaccaccg tcacgcgtca gcggcgtcca gccaattgcc gcacggctac gtgtcgggcc
    79921 agacgagaag agatccagcg gtacagagac atacacgcct ttggtaaagt ccccttcgcc
    79981 gtactcctct ttcgaaacgt tggtgatcgt ggcatagcca cccaccacca cgccgctatc
    80041 aaagcgtttg gcgatctcca gcgtgccgcc tttatcccct gccagatact gcccgacgct
    80101 cgctttcact aacacatcct gagcaaaaga tggcgtccag tatgcggtca gatgtccggt
    80161 tttcacgctg tagtcggtga atttcatcat atcttttgca ctacgccagt cgcgctgttt
    80221 aacgtagttg gcatccagac caaacgccca gttgctgtcc agcgggcgat acaacacttc
    80281 tgcccccgca ccgccaaaca tggtttcgag ataacctccg tagacctgac cgtagaagcc
    80341 gttgcccaga tgctggaagt agttggcttg caggttattc acatagacat cgttctgcac
    80401 atactcgcgc acatgggtac gaacgcgcgg taagtgcgag tcctgcggcg gattggtgta
    80461 gttaaacttg tcgtagttgt tggcaaggtt agcaaacagg ctgccggtgg tcagcagatg
    80521 gtccgtcagc cacaaatccg ccgttcccat cacgcccagc tgatacatgt aaaagttttc
    80581 cgggccaccg accgactggt tcaacaccgg atcgatatgg aaatcaaagc gtgatttgtc
    80641 gatataccag ccctgctcgg tggactgcgg aaccactggc tcgacgcgtt tttgcgccag
    80701 cttcgtttcg tggcccaacg gctctccggc gagatgattt ttcaggctgg cgacatcggt
    80761 ttccgtcgtc acctgcggca tgttaaggcg attttccgta atgcggatcg ttttgatccc
    80821 atccggcaga tcgttcatca cgatccgatt agcacggatg atcccttcac gcgaatcacg
    80881 atatttcacc tgctcgccgg tgacatacag cgtatcgcct ttcgtctgga tctgcggatc
    80941 ggccagtccg gcgttgtatt tcaacagcgt taactgattc gccaccaccg aatgctgcaa
    81001 aatggcatcc tgtggttgcg gttgatattg cgggcgggcg ttatcgttgt aggacgggcg
    81061 cagatcgtta aagttggtgc gcagcgtgac gccaaacata aaagtgttgc cacgttcata
    81121 gctaaggtta acgtcggccc aatcggtaac gcgataaatc gccccgacgt taaacttact
    81181 tttttgctcc agcttaccgg caaaatcctg ctgataatta ttgccttcat actccagttt
    81241 caggcgcagc ggttgccagg gcgtctggta ttccacgccg ccaaacagtg aggccggacc
    81301 gtggaacatc tggctgccgt cgatagatcc cgcttgtttg tagctgttat cgcgatagca
    81361 atatttgtca ctggccgaac agagcggatt tttcacatta ccgctggtgc ccagatatcc
    81421 ccagcccagg ccgagcgtaa aatcgaatgg cccccaggct ttgctggcga caagatattc
    81481 cgcatcgaac agccccgtac cgccgatatc ccgcgcacca accgccactt gcggcagcca
    81541 gtaactctct tcccacaaac gcagtttgag atcgaaggct ttatctttat acgtttgatc
    81601 gccagagaac gcttcgacgc tgctgtactg ccgggtgcgc acgtcggtgt agcgcagcgt
    81661 tgtttccagc cacgggaaga gttgcactga agctgagtaa taacggtact gatcgttatc
    81721 gcgatagttc agactcagct ccccttcccg cgccatgcgc gcggtgggcg tttgtaataa
    81781 tcctacgcca ccgaaatccg attgcgacgg accaatgggc gcaggatatg tttcgccgta
    81841 gcaagctgtg ctaataccca gcgccagtaa gctaagcaga tgtctttttt tcattattgg
    81901 ggtatccgct gcgtcagagt ctgaagaatg tcggcgttaa gggcatcagg tgtctcactc
    81961 cagacggagt ccgcgaggcc aacataaata atgctgccgg gcatcggctc tacgtgacgc
    82021 ttgttccagt aagccaccgg cgctttttgc gtgcgtccgt ccgggtaaac cacccacgcg
    82081 tagctgcgat ccgcaccgct aagcaggttc tgcccggaga gatagctcgc cacgtcgcga
    82141 cctggagtga atggctgctt gccaggacgg ctgataagcc cgaacaacgt gacagtggac
    82201 ggtggtggtc cgacccacag tgtgtagttg ccctgcaacg gtgggttacc acgttcggca
    82261 acgcgcacga tatcgggatc aagattgatt ttttgcctgc ccgtcacttt caacgcctga
    82321 atttgctggc gtaaggcgtt aatcgcagcg gcatcgtcag cgctggaatc tgcggccagt
    82381 tctgccagcc gtgccagtag cgcctgctgc tgccgcaatg ctgccgccgt tgccagctct
    82441 tcgctgatca ctgcaccagg ccaccagctg tttgccagtc gaggttgccc taccagatcg
    82501 attaaatgct ctgcgcccgt tagcgttttg gcctcactgc tgccattgct gaacaccttg
    82561 acggttcctg ccgcgaagac cgatgacgcc cccacactca aaatcaatgc gacaatagtt
    82621 tgtttaatca ttgttttgcc gccttgatga gagtggtttt caccgggaaa taatccgcgc
    82681 cgagatactg ttccgactgg cgaatttgcc cttcgctgtc gatccagtag cggttatgcc
    82741 agcgcgcctg gtcggtggag acttcttcgt ccagcacgcg aaccggggtt tcatcgctgc
    82801 cgaccttgac ggtatcggtg ccatcccatt tgaagactga gcgtgcggtg gcgtagcgta
    82861 cctgctggta ctcgatccag cccatcgtgc gcgtccatgt tgcaccatca acaatttgtg
    82921 cgggtttaat cagcgggtcg gcggcaaggt tattcacttc aatcaggttg tcgccgccaa
    82981 gcaaagtctt caccagacga ccatgttgtg tgacgagagt ggcctgatcc tgagtgaccc
    83041 atttttgttg tccgtcttca gcgaaggcca gcaccacaaa taactgcggc ccgccattaa
    83101 gctgcatgta ctggctggcg tagggcatat tttgaatatc atcgtccgtc agctgtacgc
    83161 ctggcgtgcc gaacagactg tcccacagtg aattgcccag ctctttagtc gtggctgaac
    83221 aggcctgtaa tagcaggcag ataagaatga gtgcaggtcg cttcacgact cttctccaga
    83281 ggggcagaat aaccacaccg gagtgtggtt atggttaatc cccttcatac ttcaagctgc
    83341 agatgcggtg accgcactcg ttcaccccgg tcacatagtt atctatgctc ctggggactc
    83401 actcgcttgt cgccttcctg caactcgaat tattttgggg atacatactt tattactggg
    83461 tactggtggt cgtggttgtt gtggtcccgg tattagaacc atcaccgcca ccgcttgccg
    83521 cgagcgcgac acccaccgcc gagcttacgg tgctgacgct ggtcgcggag gaactccccg
    83581 ccgaaaccga cgttgctgcc gaccctgccg cttcgcccac ctgtacaggt gcagcccacg
    83641 cagaagtcgc cgcaagcgca gatatggcaa aaatgccata cagaactttt ttcataacaa
    83701 tttcccttca ttgaatgaat ggagatttac ccaaaaacat ttcgggcggg atcgagtata
    83761 cacaactaaa gcatgcgaat tacggcgcgg ggaattagtg atggggtttt aggataattg
    83821 gaatgaatat caaatataag cttcagctta tatataaata gcactaaaaa tcataagctt
    83881 aaaataaaaa taaaagtaat taatctgcgt attttccgaa atcaatcacg attaaaaatc
    83941 tattttagga ttaacctcac ggtatgattt ccgttaaatc acagacaagg ggatttatct
    84001 gataaaaaat gccgacataa cgtcggcatt gtttttaagg ctatttaatc aatcgtacta
    84061 tgtattaatt gccgaatacg atgtacatat cggcatcgac ctgtaggcct gataagacgc
    84121 gaccgcgtcg catcaggcat cggttgccgg atgcggcgtg aacgccttat ccggcctaca
    84181 tatcgacaat gattaaccgc gccacgcttt atagcggtta atcagaccat tggtcgagct
    84241 atcgtggctg ctgatttctt tatcatcttt cagctctggc agaatacggt tcgccagctg
    84301 tttacccagc tccacgcccc actggtcgaa ggtgaagatg ttcagaatca cgccctgagt
    84361 aaagattttg tgctcataca gcgcaatcaa tgcacccagg ctgaacggag tgatttcacg
    84421 cagcaggatg gagttggtcg ggcggttacc ttcgaacact ttgaacggca ccacgtagtc
    84481 aagcgttgcc ggatctttac cctgatcacg atattcctgc tcaaccactt cgcgggattt
    84541 accaaacgcc agcgcttcgg tctgagcaaa gaagttagac agcagtttct ggtgatgatc
    84601 agagagcggg ttatgggtga tagccggagc gatgaaatcg cacggtacca ttttggttcc
    84661 ctggtggatc agctgataga acgcgtgctg accgttcgtg ccaggttcac cccagataat
    84721 cgggccagtc tggtaatcca caaccttacc gttacggtca acatacttac cgttggattc
    84781 catattgccc tgctggaagt acgccgcgaa acggtgcata tactggtcat acggcagaat
    84841 cgcttcagtt tccgcgccaa agaaattgtt gtaccagatg ccaatcagcg ccagcaagac
    84901 aggcaggttt ttctcggcag gcgtggtgga gaaatgcttg tccatcgcgt gtgcgccaga
    84961 aagcagttca acaaaattat caaagccgat ggagagaaca atcgacaggc caattgctga
    85021 ccacaaagag taacggccgc caacccagtc ccagaactcg aacatgttgg cagtatcaat
    85081 accaaactcg ccaacggctt tggcattggt ggaaagcgcc gcaaagtgtt tcgcaacgtg
    85141 tttttcatca ccagccgctt tcaggaacca gtcacgcgcg ctgtgggcgt tggtcatggt
    85201 ttcctgagtg gtgaaggttt tagatgctac caggaacagc gtggtttccg ggtttacttt
    85261 tttcagcact tccgcgatgt gagtcccatc gacgttagaa acaaagtgca tgttcaggtg
    85321 gtttttgtat ggacgcagtg cttcagtcac catgtacggg ccgaggtcag aaccgccgat
    85381 cccgatgttc actacgtcag tgattgcttt gccggtatag cctttccact caccggaaat
    85441 aatcgcttcc gagaaggttt tcatcttctc cagcaccgcg ttgacttctg gcattacgtc
    85501 tttgccatca accagaatcg gggtattgct acggttacgc agcgctacgt gcagcacggc
    85561 gcggttttca gtgcggttga tcttctcgcc agagaacatc gacttaatcg cgcccgccag
    85621 atcgcactct ttcgccagat cttgtaattt cgccagcgtc tcttcagtga tgcggttttt
    85681 ggagtaatcc accagcatct gatcgtcgaa tgttgcggag aacttagaaa aacgatcgcc
    85741 gtctttagca aaaagatcgg cgatcgtaac gtctttcatt tcatcgaagt gtttctgtag
    85801 tgcctgccag gcagcggtct gcgttggatt gatgtttttc attagcaata ctcttctgat
    85861 tttgagaatt gtgactttgg aagattgtag cgccagtcac agaaaaatgt gatggtttta
    85921 gtgccgttag cgtaatgttg agtgtaaacc cttagcgcag cgaagcattt attagctgaa
    85981 ctactgaccg ccaggagtgg atgaaaaatc cgcatgaccc catcgttgac aacggccccg
    86041 ctcacccttt atttataaat gtactacctg cgctagcgca ggccagaaga ggcgcgttgc
    86101 ccaagtaacg gtgttggagg agccagtcct gtgataacac ctgagggggt gcatcgccga
    86161 ggtgattgaa cggctggcca cgttcatcat cggctacagg ggctgaatcc cctgggttgt
    86221 caccagaagc gttcgcagtc gggcgtttcg caagtggtgg agcacttctg ggtgaaaata
    86281 gtagcgaagt atcgctctgc gcccacccgt cttccgctct tcccttgtgc caaggctgaa
    86341 aatggatccc ctgacacgag gtagttatgt ctgaaattgt tgtctccaaa tttggcggta
    86401 ccagcgtagc tgattttgac gccatgaacc gcagcgctga tattgtgctt tctgatgcca
    86461 acgtgcgttt agttgtcctc tcggcttctg ctggtatcac taatctgctg gtcgctttag
    86521 ctgaaggact ggaacctggc gagcgattcg aaaaactcga cgctattcgc aacatccagt
    86581 ttgccattct ggaacgtctg cgttacccga acgttatccg tgaagagatt gaacgtctgc
    86641 tggagaacat tactgttctg gcagaagcgg cggcgctggc aacgtctccg gcgctgacag
    86701 atgaactggt cagccacggc gagctgatgt cgaccctgct gtttgtcgag atcctgcgcg
    86761 aacgcgatgt tcaggcacag tggtttgatg tacgtaaagt gatgcgtacc aacgaccgat
    86821 ttggtcgtgc agagccagat gtagccgcgc tggcggaact ggccgcgctg cagctgctcc
    86881 cacgcctcaa tgaaggctta gtgatcaccc agggatttat cggtagcgaa aataaaggtc
    86941 gtacaacgac gcttggccgt ggaggcagcg attatacggc agccttgctg gcggaggctt
    87001 tacacgcatc tcgtgttgat atctggaccg acgtcccggg catctacacc accgatccac
    87061 gcgtggtttc cgcagcaaaa cgcattgatg aaatcgcgtt tgccgaagcg gcagagatgg
    87121 caacttttgg tgcaaaagta ctgcatccgg caacgttgct acccgcagta cgcagcgata
    87181 tcccagtctt tgtcggctcc agcaaagacc cacgcgcagg tggtacgctg gtgtgcaata
    87241 aaactgaaaa tccgccgctg ttccgcgcgc tggcgcttcg tcgcaatcag actctgctca
    87301 ctttgcacag cctgaatatg ctgcattctc gcggtttcct cgcggaagtt ttcggcatcc
    87361 tcgcgcggca taatatttcg gtagacttaa tcaccacgtc agaagtgagt gtggcattaa
    87421 cccttgatac caccggttca acctccactg gcgatacgtt gctgacgcaa tccctgctga
    87481 tggagctttc cgcactgtgc cgggtggaag tggaagaggg tctggcactg gtcgcgttga
    87541 ttggcaatga cctgtcaaaa gcctgcggcg ttggcaaaga ggtattcggc gtactggaac
    87601 cgttcaacat tcgcatgatt tgttacggcg catccagcca taacctgtgc ttcctggtgc
    87661 ccggcgaaga tgccgagcag gtagtgcaga agctgcattt taatttgttt gagtaaacac
    87721 agtatggccc ggaagacatt atttcgggcc gtattgtttt tcttgtcatt atgcccataa
    87781 ataaacgagc ctgtactctg ttaaccagcg tctttatcgg agaataattg cctttaattt
    87841 tttatctctt tctctaacta attagagaaa gagataaata gttaagagaa ggcaaaatga
    87901 atattatcag ttctgctcgc aaacgaattc ccgtacactg aactctctgg ctcactgagg
    87961 gcaccatcat ggcactcccc cgtattaccc aaaaagagat gactgaacgc gagcagcgtg
    88021 aacttaaaac actgctggat cgggcgcgga ttgcgcatgg tcgcgtactg accaactccg
    88081 aaaccaacag cattaagaaa gagtacatcg ataaattaat ggtcgagcgt gaggctgaag
    88141 cgaaaaaagc ccgccaattg aagaaaaagc aggcttataa accggatcct gaggcgtcat
    88201 ttagctggtc agcaaatacg tcgacgcgcg gcaggcgctg attaacgccc cttctttttg
    88261 cgccccggcg aggtaaaacg cttaccgttg gacgccgggc gaccgccttt ttccgccgtt
    88321 ttttccatct taacgactgg acgtttgatg cctaccgttt tcggtttggc cttcgcttta
    88381 ggttttgcct cggaagagga attttcgatg agcttaaaga ggtcgattaa ctcatcgtcg
    88441 gttaaatcgc gccactcccc cagcggaatg ccgcttaaac tgacgttcat aatgcgcgta
    88501 cgttccagct ttttcacttc gtaaccaaag tgctcgcaca tgcgacggat ctgacggttc
    88561 agcccctgca ccagggtaat gcggaagaca aatggcgctt cttttttgac tttgcacttt
    88621 ttagtcactg tcccgaggat tggcaccccc gcccccatac cacgaataaa ttcatcggta
    88681 atcggtttat cgaccgtcac cagatactct ttctcatgat cgttacccgc acggaggatc
    88741 ttattcacca gatcgccgtg attggtgagg aaaatcagcc cctgggagtc tttatccagg
    88801 cggccaatcg ggaatacgcg tttgctgtgg ttaacgaaat cgacaatgtt atcgcgctcg
    88861 ccatcttcgg tggtgctgac aataccgacg ggcttgttca gggcgataag taccaaatct
    88921 tcggcttccc gaggttcaat caactgacca tttactttca caacgtcgcc gggtttcacc
    88981 tgatcgccaa tggtggctcg cttgccatta aggaacacat tgccttgctc gatatagcga
    89041 tccgcttcgc ggcgtgagca aattccgctt tcgctgatgt atttatttaa acggactgat
    89101 gagtcgggca gcataaattc tcctgtaaaa cgcggaatat accgtacctc ttagcgggga
    89161 aaaacagggt tatgcggcaa cgtcgaaatt aaatgtgatc tgccacgcct tttatcacaa
    89221 aagtgtctcc ggaagtacga tgcaaatttg cctggcaaaa taaggataat caacatatta
    89281 cgaatcgctg cacacctgtg ctgatgacta ctacgcagag aaacaaatgt gcaagagaaa
    89341 gcgctatttc tccccatgca aagagtgaaa aatcgctttg aaaggccctc atgatggggt
    89401 tatatcagat caagcggtga gaaagcgtat ttgcacaaat gtgcagaacc aggaggcggc
    89461 aatggaaaac agtgacgata tccgtttgat tgtgaagatt gcccaactct attacgaaca
    89521 ggatatgacg caggcgcaaa tcgcgcgcga actgggtatt taccgcacca ccatcagccg
    89581 cttgcttaaa cgaggccgcg atcagggaat tgtcaccatc gccatcaact atgactacaa
    89641 cgaaaatctc tggctggagc agcaactgaa gcaaaagttt ggcctgaaag acgttgtggt
    89701 ggtgtcagga aatgatgagg atgaagagac tcaactggcg atgatggggt tacacggcgc
    89761 gcaactgctg gatcgcttgc tggaacctgg cgatattgtc ggtttttcct ggggccgcgc
    89821 ggtgaacgca ctggttgaaa acttgccgca ggcggggcaa tcgcggcagt taatctgcgt
    89881 accaattatt ggcggaccgt ccggtaaact cgaaagccgc tatcatgtaa acacattaac
    89941 ctacagcgcg gcagcgaagt tgaaagggga atcgcatctc gcggattttc cggctctgct
    90001 ggataaccca ttaattcgta atgggatcat gcagtctcag cactttaaaa ccatctctgc
    90061 ctactgggat aatctggatg tcgccctggt gggaattggc tcaccggcca ttcgcgacgg
    90121 cgctaactgg catgcgtttt atggtggtga agagagtgac gacctgaatg cccgccaggt
    90181 tgctggcgat atttgctcgc gcttttttga tattcacggc gcaatggttg aaacaaatat
    90241 gagcgaaaaa acactctcta tcgaaatgaa taaattaaag caggcgcggt attccattgg
    90301 cattgccatg agtgaagaaa aatacagcgg aattattggt gcactgcgtg gaaaatatat
    90361 taattgtctg gtaacgaata gcagcacagc tgaactatta ctgaaataaa aatcttcccg
    90421 atgtttaccg taataaaaat tacggcgggt ctctattatc taaaatcagg agtctgttat
    90481 gcaaacgtgg ttaaatttgc aggataaaat cattattgtc accggtggcg catccggcat
    90541 tggtctggcg attgtggatg aattattagc acaaggcgcg aatgtacaga tggtcgatat
    90601 tcacggtggc gatggacaat atgaaggtca taaaggctat cagttttggc cgaccgatat
    90661 ttccagcgcc aaagaggtaa atcatacggt agcggaaatt atccagcgtt ttggtcgcat
    90721 cgacggtctg gtcaataacg ccggggtcaa tttcccgcgt ctgctggtcg atgagaaagc
    90781 gcctgccggg cagtatgaac tcaacgaagc tgcattcgaa aaaatggtca atatcaacca
    90841 gaaaggcgtt tttctgatgt cgcaggcggt ggcgcgacag atggtcaaac aacatgatgg
    90901 cgtgattgtg aatgtttcct cagaaagtgg gctggaaggc tcagaaggcc aaagctgtta
    90961 cgccgctacc aaagccgcgc ttaatagctt cacgcgatcc tggtcgaaag aattgggtaa
    91021 acacggtatc cgtgtagtcg gtatcgcgcc ggggattctg gaaaaaacca ggctacgcac
    91081 gccggaatat gaagaggcgc tggcgtggac gcgcaatatc accgtcgagc agctgcgtga
    91141 aggctatacc aaaaacgcca ttcctattgg gcgcgccgga agattagcag aagtcactga
    91201 ttttgtttgt tatctgctgt ctcaacgcgc cagctatatc accggagtaa ccactaacat
    91261 tgcgggcggc aaaacgcgcg gctaaggagg ctttatggtc aacgccattt tttgtgccca
    91321 cggcaaatta gcttgcgcca tgctggaatc ggttcagatg gtttacggca acgccaatgt
    91381 tgaagccgtt gaatttgtgc ccggtgaaaa cgccggagac atcgtagcaa agctggaaaa
    91441 gttagtaagc attcacaatc aagatgagtg gctgattgcc gtggatttac agtgcggcag
    91501 cccgtggaat gcggcagcga tgcttgcgat gcgcaatcct cgtctgcggg taatcagcgg
    91561 cctttctctg ccgctggcgc tcgaactggt tgataaccag cacagcatga atgttgatga
    91621 actgtgtgaa cacctgacgc aaatcgcaaa gcaaacctgc gttgtctgga aacaactggc
    91681 aacggcagag gaggatttct gatgaacatt acgctcgccc gcattgatga ccgtctgatc
    91741 cacggccagg tcactaccgt ctggtcgaaa gtggctaatg cccagcacat tattatctgc
    91801 aatgacgaag tttataacga tgaagtgcgc cggacattat tgcgtcaggc tgcgccaccg
    91861 ggaatgaagg ttaacgtggt caatattgaa aaagccgtcg cggtttatca caacccgcaa
    91921 tatcaggacg aaaccgtttt ttatcttttc actcgcccac aggatgcctt agcgatggtt
    91981 cgccaggggg taaaaataga caccctgaat attggcggca tggcctggcg gcccggtaaa
    92041 aaacagttaa ccaaagcggt ttcattagat gatgatgata ttaacgcatt tcatgagtta
    92101 aataatcttg gcgtcatact ggatttacgc gtcgtagcgt cagatccctc aatcaatatt
    92161 atcgacaaaa taaacgaaca gttaatcgca aattaaataa tagcgcctgt gtacatatgc
    92221 tttaacaggc agggatgacg acacttaaaa ggtgacaaat catggaaata agtaccctac
    92281 aaatcattgc tatatttctt ttttcctgta ttgccggaat gggcagcgtg ctggatgaat
    92341 ttcagactca tcgtccgtta attgcctgta cggtgattgg tttaattctc ggtgatttaa
    92401 aaaccggaat tatgctcggt ggtacgctgg aattgatagc tctcggctgg atgaacgtcg
    92461 gcgcggcgca atctccggat tctgcactcg ccagcataat ctccgccatt ctggttatcg
    92521 ttggtcagca aagcatcgcc accggaatcg ccatcgcgtt gcctgtggct gcggcaggcc
    92581 aggtgctgac ggtgtttgcc cgtaccatca ccgtagtgtt ccagcacgcg gcggataaag
    92641 cagcggaaga agcacggttt cgcactctcg atattctgca tgtctccgcg cttggcgtgc
    92701 aggcactgcg cgttgctatt ccggcactga ttgtctcgct gttcgtcagc gctgatatgg
    92761 tgagcaatat gctgagcgcc attcccgaat ttgtgacccg tggactgcag attgctggcg
    92821 gttttatcgt ggtggtcggt tacgccatgg tgcttcgcat gatgggcgtg aaatatttga
    92881 tgcctttctt tttcctcggt ttcctcgcag gtggctacct cgatctcagt ctgctggcgt
    92941 tcggtggcgt cggcgtgatc atggccctgc tctacatcca gctaaatcca cagtggcgta
    93001 aagctgaacc tcaactccag gccaccgcct ccaccgccct tgaccaactt gacgattaac
    93061 ggagccatca tggaacagag aaaaattaca cgcagcgatc tggtgagcat gtttctgcgc
    93121 tccaacctgc aacaggcgtc ctttaacttt gaacgtattc acgggctggg tttttgctac
    93181 gacatgatcc ccgccatcaa gcgactttac ccattaaaag aggatcaggt tgcggcgctc
    93241 aggcgacacc tggtgttctt caataccacg ccagccgtat gtggcccggt catcggcgtc
    93301 actgccgcca tggaagaggc gcgggccaac ggcgcggaaa ttgatgacgg taccattaac
    93361 ggcatcaaag tcggtctgat gggaccattg gcaggagttg gcgatccact ggtctgggga
    93421 acgctgcgcc cgattaccgc cgcgctcggc gcatctctgg cactttcggg caacattctc
    93481 ggcccgctgc tgtttttctt tattttcaat gcggtgcgtc tggcgatgaa gtggtatggc
    93541 ctacagctcg gctttcgcaa aggggtgaat atcgtcagcg atatgggcgg gaatgtgctg
    93601 caaaaactca ccgaaggcgc gtcgattctc ggactgtttg tgatgggcgt gctggtgacc
    93661 aaatggacgt caatcaacgt accgttgctg gtttcacaaa cgcatgccgc cgatggctcc
    93721 accgtcacca tgaccgtgca gaacattctc gaccaacttt gccctggttt gctggcgctc
    93781 ggtctgacgc tgctgatggt tcgtctgctc aacaaaaaaa ttaacccggt atggctgatt
    93841 ttcgccctgt ttggtttagg gattatcggt aatgcgctgg gcttcctgtc ctgattcttc
    93901 gccccggcac gactgccggg gtcatcgctc aacatgaggt ggtttatgaa aacaacagct
    93961 ctgcgtcttt atggcaaacg tgacctgcgt ctggaaacct ttgaccttcc tgaaatgcag
    94021 gaggatgaaa tcctcgcaac ggtggtcact gacagcctgt gcctctcttc ctggaaagag
    94081 gccaatctgg gtgaaaacca taaaaaagta cccgacgatg tggcgaccaa ccccatcatc
    94141 atcggccacg agttttgcgg cgatattctg gccgtgggta aaaagtggca gcacaaattc
    94201 cagccgggtc agcgttatgt gattcaggcc aacctgcaac tccccgaccg cccggattgc
    94261 cccggctact ccttcccgtg ggtaggcggc gaagcgacgc atgtggttat tcccaacgag
    94321 gtcatggaac aagattgcct gctggcatac gacggcgaaa cctattttga aggctcgctg
    94381 gttgaaccgc tttcctgcgt gattggcgcg ttcaacgcca actatcatct tcaggaaggt
    94441 agttataacc acacgatggg gattcgcccg caagggcgca cgctgatcct cggcggcacc
    94501 ggcccgatgg ggctgctggc gattgattat gcgttgcatg gaccggttaa cccgtcgctg
    94561 ctcgtcatta ccgataccga caacgacaaa ttgagttatg cacgcaagca ctatccatca
    94621 gaaccgcaaa cactgattca ttatctcaat gccgccgatg cagcatttga tacgttaatg
    94681 gcactgagtg gcggtcacgg cttcgatgat attttcgtct ttgtgcctaa tgaaggactg
    94741 gtgactctcg cctcttcctt gctggcgaca gatggttgtc tgaatttctt cgccggaccg
    94801 caggataagc acttcagcgc gccaattaat ttctacgatg tgcattatgc atttacccac
    94861 tacgtgggca cgtcaggcgg caataccgac gacatgcgcg cagcggtcaa attgattgaa
    94921 gagaaaaaag tgcaggccgc aaaagtggta acacatattc ttgggctgaa tgccgcgggc
    94981 gaaaccacgc ttgaattgcc tgccatcggc ggcggcaaaa agctggtgta taccgggaaa
    95041 tacctgccgc ttatgtcact cacgcagatt caggatcaag cactggcggc aattctggcg
    95101 cgtcatcagg ggatctggtc cggtgaggcg gagcaatacc tgctcgccca tgcagaggca
    95161 atttcccatg attaatcgcg ctacttaact ctgcatgtcg ctggctggtc gccgcggcaa
    95221 cttcgcgacc tgatgtgcgg ctgattgctg aagcggcaca atgtgcacga gaaggataaa
    95281 aaaacatcag cgccttaagg ggcgctcaaa ttggtggcaa agagcacatt gtttatgccg
    95341 gatgcggaat gaagccttat tcggcctaca taatcttgaa aattcaatat attgcagtta
    95401 tctggtaggc caggtaagcg tagcgcatca ggcaatcctg tgtttgtcat cagtctcagc
    95461 gcctgtgcaa tatgtgacat cgtgagccat gtcagtaagt ggttaaattc gcagtggtag
    95521 cattccttcc gtgttttctt actaaccgga gaagtaatgg aactgctttt attgagtaac
    95581 tcgacgctgc cgggtaaagc ctggctggaa cacgcgctgc cgctgattgc tgaacagttg
    95641 cagggtcgcc gttctgcggt atttattcct ttcgcgggcg taacgcagac ctgggatgat
    95701 tacacagcga aaacggctgc ggttctcgct ccgctgggtg tttctgtcac cggtattcat
    95761 agcgttgtcg atcccgttgc cgcgattgaa aatgcggaaa ttgtgattgt cggcggcggt
    95821 aatactttcc agttgctgaa acagtgccgc gagcgcggac tgctggcacc aattactgac
    95881 gtggttaaac gtggcgcgct gtatattggc tggagcgctg gcgctaacct tgcttgccca
    95941 accattcgta ccaccaacga tatgccgatt gtcgatccac aaggtttcga tgcgctgaat
    96001 ctgttcccgt tgcaaatcaa cccgcacttc accaacgcgc tgccggaagg ccataaaggt
    96061 gaaacccgtg agcagcgtat tcgcgaactg ctggtcgtcg cgccagaact cacaattatt
    96121 ggtctaccgg aaggtaactg gatcacggtg agtaaaggtc acgctacgct gggtggcccg
    96181 aacaccactt atgtgtttaa ggctggtgaa gaagcggttc cgctggaagc gggtcaccgt
    96241 ttttaagtga attgccggat gcatgatccg gcatcttgta catagtgcct gatgcgacgc
    96301 tttacacgtc ttatcaggcc ttcagggtca cggtattaat actcgtcccg tccttcgtct
    96361 tcatccggct gttccagcac actgtaagcc accgaacaaa acagcgagtt cagacgctgc
    96421 atatctccca gtaagcctaa atgtagcgaa ctggtttcaa tactctgcac gttttgctga
    96481 tgcaggcgat cgacgtgagc atgggaataa cggcgattaa gaatgcgaaa gcgatgcttg
    96541 ctgcggcgca aacgacgggc gctggtaaca tcgccagaga agaaaaccga cattgccagt
    96601 tttaaattac tgagcaattg ctcatagagc gcatccagtt ccttcaagcc atcaagcgaa
    96661 aatgcccgcc gtgctgccag cgatttatcg gcaatttcac tccccatgcg ctcgacgata
    96721 tcggaggcct gttcaaggtt gagcgacatt tcgatgatct ccgcccagcg gcgcgactct
    96781 tcttctgcca gttcctcttt tggcatccgc gccagataca gcttaatggc ggtatagaga
    96841 acgttgatgt catcagccag cttacgcagc tctttctcct gccgtggctc gccgtgcatc
    96901 actttattca acccttccat catctgttcc atggcgtcgc caatgcgcag ggtttcgcgg
    96961 gcagcgttcg ccagagcaag cgtgggcgta tccagcgcgc tgacatccag atgtttaggc
    97021 cgtagctggg tatccagttc cggttcatcg cgaataatcg ttttgcaaaa ccgtgccatc
    97081 gggtcaacaa atggcagcat gaccaggcag cgtacaaggt tgtagaagac gtggaaatag
    97141 atcaccaatt ccgcttttgg caatggcaac ttccccattg tctctgccag caaatgaaca
    97201 aacggcagga taatcaggct acccaccagc ttaaacagca ggctacccag cgcgacacgg
    97261 cgggctgcgg cattggcggc actgttgttg agcatcgcca gcaggccgga accgaggttt
    97321 gcaccaatca ccagacaaag cgccactggg aaagagataa tgcctgcggc ggtcagagtc
    97381 gcggtcagca gtaccgcggc aaggctggag tagctgataa tggcgaacat cgcaccaatc
    97441 agcgcatcca gcagaatatc gccggtcagc gaggcaaaga tcacctgaac gccgtttgcc
    97501 tgggtgatcg gcgttacggc ctgcacaatc aactccagcg ccagcaaaat caacccaaga
    97561 ccaataccga cgcggcccag ttgcccggcg cgtgactgtt tgcgtccgag gaaaaaaatc
    97621 acgccgataa aaataagtaa tggtgacagc caggataagt cgaaggtgag aatacgcgcc
    97681 attagcgccg tcccgacatc tgcacccagc acaatcacca gagccggtgc gagggctacc
    97741 agatcctgag cgacgaacga ggtcaccagc atagtggtgg cattactgct ctgcaccagt
    97801 gcggtaacgc ctatccccgc gcaaaaggcg agcggctttt tttcgacgct ccggctaagg
    97861 acagtacgca aacgcgcgcc gaagacgcgc attacgccgg ttcgaacaat atgagtcccc
    97921 cagaccagca gggcgacggc agaaagcagg tgaagcagcg ttaacacgag ttcaggttct
    97981 ccttatcgtt atatgttcct gaagcaattg aatgaccaaa ggaccggaaa cacgctgact
    98041 ggcatcttta ctgtgccttt tgaccttcgt ttttccggct aagattattc cagcctgttt
    98101 ccagtataag tgtttcatat taataaagag acagggcact cattgagcgc cctgtttgtt
    98161 gtaaggaaaa gtgacagatt tgtgaatcag tccgcgtcat accccagatt cggtgccagc
    98221 cagcgctcaa catctgagac gctcatacct ttacggcggg catagtcttc aacctgatcg
    98281 cgctggattt gcgccaccgc gtaatacttg ctgtccgggt ggctgaagta ccaacccgaa
    98341 accgatgcac caggccacat ggcgaaagat tctgtgagtt tcatgccagt gtgtttttcc
    98401 acttccagca gctcccagat ggtggctttt tccgtatgtt ccgggcaggc cggataaccc
    98461 ggtgccggac ggatgccctg gtagttttcg cggatcagct cttcgttgct gaggttctcg
    98521 ttcggcgcat agccccagta gactttacgc acacgctcat ggagatactc cgcaaaggct
    98581 tcggctaaac ggtcggcaag cgctttcacc atgattttgt tgtaatcatc gtgctgcgct
    98641 tcaaaggcat cagccagtgc gtcctcttcc agcccgccag tcacggcaaa tgcgccgatg
    98701 taatctgctt taccagaaag cttcggcgca acgaagtcag cgagacagta gttagcgaag
    98761 ccggtttttt cggtctgttg acgcagatgg tggctgacgt tgatcacatg ggtacgcgtt
    98821 tcgtcacggt agatttcaat gtcatcgccc acacggtttg ccgggaacag gcccaccacg
    98881 ccacgcggat tcagcgtttt ttcggcgctt aatttatcca gcatgtcgtt ggcgtcttta
    98941 aacagccgct gcgcctcaac gcccaccact tcatcttcca gaatgcgcgg atacttcccg
    99001 gacagcgacc aggtcataaa gaacggtgtc cagtcgatgt aattacgcag cgtttcgatg
    99061 ctggcttcga cttcctgcac accgagacgg tgcgccaccg gcggcgtgta agcctgccag
    99121 tcaaaagcga agtcgttatc acgcgccgct tccagcgtga ccggtggtgt gcgtggtttc
    99181 tttcgtccgt gctgaatacg cacggtttcg tactccttgc gggtacgagc aacgaaatca
    99241 tcgcgctgag tgtcggaaag caacgccgcc accacaccaa cggtgcgtga agcgttctgc
    99301 acatacaccg tcgggccgct gtagttctgc tcaattttca ccgccgtgtg cgcttttgag
    99361 gtggtcgcgc cgccaatcaa tagcggaatg gtgaagccct gacgctccat ctctttcgcc
    99421 acgttaacca tctcgtccag cgacggcgtg atcagccccg aaaggccaat caaatcagca
    99481 ttcacttctt tagcggtacg gagaattttt tccgcaggca ccatcacgcc gagatcgaca
    99541 atttcgtagt tgttacattg cagcaccacg ccaacgatat ttttaccgat gtcgtggaca
    99601 tcgcccttca ctgtggcgat caccatcttg ccgttggttt taccctgctc tttgctggct
    99661 tcaatgaacg gctcgagata cgccaccgcc tgtttcatga cgcgcgccga tttcaccacc
    99721 tgcggcagga acattttgcc ctcgccaaac aggtcgccga ccacgttcat gccgtccatc
    99781 aacgggcctt caatcacttc aatcggacgc gtagcctgct ggcgggcttc ttcggtatcc
    99841 tgctcgataa actcggtaat gcctttgacc agcgagtatt ccagacgttt attcacttcc
    99901 cacgagcgcc actctgcctg ctgggtgtta gcggtgtcgt cggttttgct gccgcgatat
    99961 ttctcggcaa gctcgagtaa acgctcggtg ccatcgtcgc gacggttgag gatcacatcc
   100021 tccaccgcgt cgcgtagctc ggtaggcagg tcgtcgtaaa tcgccagttg cccggcgttg
   100081 acgatcccca tatccatgcc attgcgaata gcgtagtaga ggaacaccgc gtgaatggct
   100141 tcgcgcaccg gatcgttgcc acggaatgag aaagaaacgt tagaaacgcc gccggaaatc
   100201 agtgcgtgcg gcagttcacg tttgatgtct tcacacgcgc cgataaagtc ctgcgcgtaa
   100261 ttgttgtgct cttcaatgcc agttgcgacc gcgaagatgt tcgggtcgaa gatgatatct
   100321 tcagccggga agccaacctc ttcggtgagg attttgtacg cccgacggca aatctcgatt
   100381 ttccgtgcgc gggtatcggc ctggccctgt tcgtcaaaag ccattaccac gactgccgca
   100441 ccgtagcgac gcaacagttt cgcgtgatgg ataaaggcgt cgacgccctc tttcatcgag
   100501 atagagttaa caatgccttt gccctggata cacttcagac ctttttcaat aacgtcccat
   100561 tttgaggagt cgatcattat cggcacgcga gcgatatccg gttcaccggc aatcagattg
   100621 agaaaacgca ccatcgccgc ttcggcgtcg agcatccctt catccatgtt gatatcgata
   100681 atctgcgcac cgttttccac ctgctgacgc gccacatcca gcgcctcgct gtatttctct
   100741 tctttgatca ggcgcttgaa cttagcggaa ccggtgacgt tggtgcgttc acccacgttc
   100801 acaaacaggc tatcttcgcc aatgttcagc ggctccaggc cggacaaacg gcaggctacg
   100861 gggatttccg gcagtttgcg cggcgctaat ccttctactg cacgactcat cgcggcaata
   100921 tgttgtggcg tggtgccaca gcagccgccg acgatattga gaaagcccgc ttgcgcccat
   100981 tcacgtatct gttttgccat cgtgtcggcg tcgagatcgt actcaccaaa ggcgttgggt
   101041 agcccggcgt tcgggtgcgc tgtgacgtag cattccgcaa tccgtgataa ttcctgcacg
   101101 tactggcgca gttcgtctgg ccccagcgca cagttaaggc cgaaagtcag cgcttcggcg
   101161 tggcgcagag agttataaaa cgcttcggtg gtctgcccgg agagcgtgcg cccggaggcg
   101221 tcggtgatgg tgccggagat cataatcggc agctcaacgc ccagcgcttc aaactccgtt
   101281 ttcaccgcaa agaccgccgc tttggcgtta agggtgtcga aaacggtttc aatcagaatc
   101341 aggtcggcac caccttccac cagcgctttg gtggactctc gataagccgc caccagcccg
   101401 tcaaaagtga tattacgaaa tgccggatcg ttgacatccg gagaaataga cgccgtgcgg
   101461 ttggtcgggc cgagaacacc ggcaacgtag cgcggtttct ccggcgtgcg cgcggtccac
   101521 tcatcagcac aggcgcgcgc cagtttcgcc gccgcaaagt tgatttccgc cgacaggaat
   101581 tccatctggt aatccgccat cgcaatggtt gtggagttga aggtgttggt ttcgatgata
   101641 tccgcgcccg cttcaaagta ggcgttgtgg atagcggcga tcacttccgg tttgctgagc
   101701 accagcaggt cgttgttgcc tttgagatcg catggccagt cggcaaagcg ttcaccacga
   101761 aaatcggctt cgttcagtcg atagctctgg atcatggtgc ccataccgcc gtccagcacc
   101821 agaatacgtt catttaactg cgcacgcagt tgttccactt tgctgctcac acttgctccc
   101881 gacacgctca acccagattt aataaaaatt caacaaacca tactggcata aacgcatctg
   101941 tggtaaaagc gaccaccacg caacatgaga tttgttcaac attaactcat cggatcagtt
   102001 cagcaactat tgcattagct aacaataaaa atgaaaatga tttccacgat acagaaaaag
   102061 gagactgtca tggtcgcacc cattcccgca aaacgcggca gaaaacccgc cgttgccacc
   102121 gcaccagcga ctggacaggt tcagtcttta acgcgtggcc tgaaattact ggagtggatt
   102181 gccgaatcca atggcagtgt ggcactcacg gaactggcac agcaagccgg gttacccaat
   102241 tccacgaccc accgcctgct aaccacgatg cagcaacagg gctttgtccg tcaggtcggc
   102301 gaactgggtc actgggcaat cggcgcacac gcctttatgg tcggtagcag ctttctccag
   102361 agccgtaatt tgttagcgat tgttcaccct atcctgcgca atctaatgga agagtctggc
   102421 gaaacggtca atatggcggt gcttgatcaa agcaatcacg aagcgattat tatcgaccag
   102481 gtacagtgta ctcacctgat gcgaatgtcc gcgcctatcg gcggtaaatt gccgatgcac
   102541 gcttccggtg cgggtaaagc ctttctggcc caactgagcg aagaacaggt gacgaagctg
   102601 ctgcaccgca aagggttaca tgcctatacc cacgcaacgc tggtgtctcc tgtgcattta
   102661 aaagaagatc tcgcccaaac gcgcaaacgg ggttattcat ttgacgatga ggaacatgcg
   102721 ctggggctgc gttgccttgc agcgtgtatt ttcgatgagc accgcgaacc gtttgccgca
   102781 atttcaattt ccggaccgat ttcacgtatt accgatgacc gcgtgaccga gtttggcgcg
   102841 atggtgatta aagcggcgaa ggaagtgacg ctggcgtacg gtggaatacg ctgacttttt
   102901 cgcctctgat ttatacgaat cgaacgctaa accgctgctt gcgccgatac gcataaacat
   102961 cttccacatg cccttcacgt atgcggtttt gtagtgcgcg ccagtaatca gcgcggaaca
   103021 agtcggcgtg catctcttca aatagcggac caatacgcgg gtcggcacat aaccagtgac
   103081 ggaactcttc cgggaaaaca tcgcccggcg agacgctgta ccacggttcg ctggcaagtt
   103141 cgtcctccgg gtagcgcggc ggcgggatgt cgcggaagtt cacttccgtc atgtagcaaa
   103201 tttcgtcgta atcataaaac accacgcgcc cgtgacgggt gacaccgaag tttttaaaca
   103261 gcatgtcgcc agggaaaata ttagcggcgg caagctggcg aatagcgtta ccgtattctt
   103321 caatggcatc gcgcaactgc tgaccttcca cttgttccag ccagatattg agcggcacca
   103381 tccgccgctc aatataaaga tggcgaatca caatttgttc gccgagatcg gtgatttttt
   103441 ccgctgcttc ctggagcagt aatgccatta atgccgggga aatatgccgc ttctccagca
   103501 caaagttttc aaactcctgg gtgtccgcca ttcggcccac gcgatcgtgc tctttcacca
   103561 gttgatagca ggcacgaacg tgagcggcag acatctcttt ctgcggcgcg aacttgtctt
   103621 tgatgacttt gaatacccga tcaaagcccg gcagcgtaaa caccagcatc accatgccac
   103681 gaatacccgg cgcttcaatg aactgctcat tacagccctg tagataaacg agatattcgc
   103741 ggtagctttc ggttttggcg tgcttctggc agccgatagc catatacaat tcagcggtgg
   103801 ttttacctgg cagaatttcc cgtagccact cgaccagcgc tgcgggcagc ggtgcataaa
   103861 ccataaaata agaacgcgca aagccaaaaa caatgctcgc ttcggcggtg gtcgtcaggc
   103921 aggtatcaat aaataactcg ccgtcgtccg tctggtggat cggcagcaaa aatggcaatg
   103981 tgccggaagg cgtgatcaga ttgcctacca gccaggcggc tttattgcgg taaaacagtt
   104041 cgttcgccac ctgtaaatga ctttccgcga ggttgtctgt ccccagcgtt tccgtcagat
   104101 ggcgaacgat gtaatgaatg tcacggcttt tattctgcca acgcagacgc agcggcagat
   104161 cgctgataac gcgcatcagc agagattccc agccgtgatc ggggtgaaag tctttcgcca
   104221 gcgggcgggg aatggtacga aaacggcgct ctggctggga gctaaagata aaaagccgct
   104281 cgggagtaag cgagcggtgg tcaaataacc tacagtacac ggagttgaaa aagctctccg
   104341 caatctcgaa gcgcgggtaa tccggcaaca gccgcgtgta atgctctttg acgcgcagta
   104401 aaaatgccgc gtccgtgctt tggccgttgg taatgcagcg cagttgctcc acgaccagac
   104461 ccacgtgatg atcgtaaaga tgaatacggt ttttcatcgc ctgctggaca gcatgccagt
   104521 cggcttgctc gaaacgctgc tgcgccccgg aagtcacttc gaggaatcga ccatactgag
   104581 cgtcgaaacc ttgcaaaatg gtttgtgcaa tcagtaattc caggccacgc ggcatttgtt
   104641 gccctcgtcg ggagcagaga attgccggat aagacgcgta agcgtcacat caggcgctga
   104701 ttgccggatg cggcgtagac gccttatccg gcctacgttc ggcaacggct gtaggcatga
   104761 taagacgcgc aagcgtcgca tcaggcgctg attgccggat gcggcgtgaa cgccttatcc
   104821 ggcctacagt cagcaacgtt tatcggttgc ttagaactgc gattcttcgg tggagccagt
   104881 cagtgcggta accgacgacg tgccgccctg aataatggtc gtcactttat cgaagtaacc
   104941 tgtacccact tcctgttggt gagatacgaa ggtatagcca tctttcacgg cggcaaattc
   105001 cggctgctgc actttctcaa catagtgctt cataccctcg ccctgggcat aggcgtttgc
   105061 caggtcaaac atgttgaacc acatgctgtg gatacctgcc agggtgatga actggaactt
   105121 gtagcccata tccgacagct gctgctggaa gctggcaata gttttgtcgt cgaggttttt
   105181 ctgccagttg aacgacggcg agcagttata agccagcagt ttgcccggat atttcgcgtg
   105241 gatggcttgt gcaaagcgac gcgccagttc caggtccggc gtggaggttt cacaccagac
   105301 caggtcagca tatggcgcat acgccaggcc acggctgatc gcttgctcaa tacccgcatg
   105361 agtacggaag aagccttcac tggtacgctc gccagtaata aattcgctgt catatgggtc
   105421 gcaatcggag gtaatcagat ccgccgcatc ggcatcggta cgagcaacca gcagggttgg
   105481 aacgcccgtc acatcagctg ccagacgcgc cgcgaccagt ttctgaatag cttcctgagt
   105541 tggcaccaaa actttgccgc ccatgtgacc acatttcttc actgatgcca gctgatcttc
   105601 gaagtgaact gccgctgcac cggcttcaat catcgctttc atcagttcaa aggcattcag
   105661 tacaccgcca aaaccggcct ccgcatcggc gacgatcggc aggaagtaat cgacatagcg
   105721 cggatcgccc ggctcaatgc ccgcggacca ttggatctga tccgcacggc ggaaagtatt
   105781 gttgatccgc tccaccaccg ctggcaccga gtttgccgga tagagcgact gatccggata
   105841 catgctggcc gccaggttag cgtccgccgc tacctgccat cccgacagat agactgcttc
   105901 aatacccgct ttcgcctgtt gcagcgcctg accgccagtc agtgcaccga ggctgttgat
   105961 gtagcctttt ttcgactcgc cgtgcagcag acgccacatt ttcgccgcgc ccagttgcgc
   106021 cagggtgcat tccggattga ctgaaccgcg taatttcacc acatcttccg cgctgtacgg
   106081 gcgagtaatg ccttcccaac gcggttgagt ccactctttc tgtaattctt caatttgttg
   106141 tgtacgggtt ttcatgtgca gatgctccat agttatgtgg tggtttacgc taacaggcgg
   106201 tagcctggca gggtcaggaa atcaattaac tcatcggaag tggtgatctg ttccatcaag
   106261 cgtgcggcat cgtcaaaacg cccctgggag aaacgttctt cgcccagttc gctggcaatg
   106321 actttcatct cttcgcccag catctggcgg aacaaggctt tagtcaccgg tttgccattg
   106381 ctcaacgttt tttgatgatg gatccactgc cagattgagg tacgggaaat ttcagccgtc
   106441 gccgcatctt ccatcaggcc ataaatcggc acacagccat tgccagagat ccacgcttcg
   106501 atgtactgca cagccacgcg aatgttggcg cgcatacctt cttcggtgcg ttcaccatca
   106561 caaggtgcca gcagctgatc ggcagtaatc ggcgcgtctt gttcgcgcat cacttcaagc
   106621 tgatttttac gggagccgag aatgtcgttg aataccgcca ttgccgtatc cgcaaggcct
   106681 gggtgggcaa tccatgtgcc atcgtgaccg ttattagctt ccagcgattt atccgctttt
   106741 actttgttga gcacctggtt attgcgctct tcatctttgc tcggaataaa tgccgccata
   106801 ccgcccatcg caaaagcacc gcgtttatgg caggttttaa tcaacaggcg tgagtaagca
   106861 ttcaggaagg gtttatccat cgtcactgcc tgtctatctg gcaggacgcg atcgggatag
   106921 tttttcaatg ttttgatata actgaagatg taatcccaac gaccgcagtt cagaccaaca
   106981 atatggtcac gcagcgcgtg aaggatttca tccatctgga acacggcggg cagcgtttca
   107041 atcagcaacg tcgccttgat ggtgccgcgc ggcagattaa agcgatcttc tgcatagctg
   107101 aagacttcgc tccaccaggc cgcttcctgc caggactggg ttttcggcag atagaaatag
   107161 ggaccgctgc cctttgccaa cagtgcctga tagttgtgga agaaatagag cgcaaaatca
   107221 aacaggctac cggggattgc ctcaccacgc caggtgacat gtttttccgg caagtgcaga
   107281 ccgcgtaccc gacaaatcaa aaccgctgga ttgggcttga gctgataaat tttgcctgct
   107341 tcattggtat agctgatggt gccgttaacc gcatcgcgca ggttaatttg cccatcgatc
   107401 actttgttcc agtctggtgc cagtgaatct tcgaaatcgg ccataaagac tttcacattg
   107461 gcgttgagcg cgttgatcac catcttgcgc tcaaccgggc cggtaatctc tacccgacga
   107521 tcttctaagt ccgcaggaat cccgcgaatt ttccagtcag catcacgaat ggaagctgtt
   107581 tccgaaataa aatcaggcaa cgttccgtta tcgatatcct gctgctgctg aatgcgcgct
   107641 gccagaagtt tattgcgttg tggcgtaaaa tgcgtcacca gctcagtcag aaattctacc
   107701 gcttcggcag taagaatatg cttctcctgc tcgccatacg gccttgtgaa agccaattca
   107761 tcggttgttg ttgcctgttc agtcatcgtg cagctcctcg tcatggatcc gaaaacttcc
   107821 ccactgtgaa cgaaggatcg ttgtgcgctt ttcgttcagc acaactaaga ctactcattt
   107881 aaaatgcaaa atcaaaaaca atttccattt taattaaaaa tacttgataa cttattgata
   107941 acaatatgaa taaaatttat tcacctactg aggtgcgttt cggaaataaa aaaggcaccc
   108001 gaaggtgcct gaggtaaggt gctgaatcgc ttaacgatcg actatcacag aagattaatc
   108061 cagcgttggg ttcatgtgcc gtagatcgta tggcgtgatc tggtagacgt aatagttgag
   108121 ccagttggta aacagcaaat taccgtgact acgccagctc gctcgcggtg tattttgcgg
   108181 atcattatgc gggaaatagt tatacggtac atccgggtct agtccggctt ccacatcgcg
   108241 gaaaaattcc tgcgccagcg tttgcgcatc atattcggga tggcccgtca caaaggcaat
   108301 tcgcttatct ttgctggcaa acagataggc atccccttct tctgtctccg ccagaatttc
   108361 cagatcggta taatcacgaa tcaacgctgc cggaaagtca gcatagcgcg aatgtggtgc
   108421 caggaatgaa tcatcaaagc cacgcgtcag aagcgcatga ggatggagaa tatgatgctc
   108481 gtaaacgcca gagagtttgt cggtgcgggt ttgcttagga atgccgtaga ggatattgag
   108541 cgcggcctgg accgcccaac agacaaacag cgtcgaggtg acgtgatctt tcgaccactc
   108601 cagcacctgt ttgatctgcg gccagtaagc aacatcatta aactccacca ggcccagcgg
   108661 cgcgccggta actatcagac catcaaagtt ttgttcctga atatcttcaa agttacagta
   108721 gaagttattc agatgctctg cgggcgtgtt gcgcgattca cgggaatcga tgcgcaacag
   108781 ctgaatatcg acctgcaaag gtgagtttga aagcaggcgc agaaactgat tttcagtttc
   108841 gatcttcttc ggcatcaggt taaggataag cacctttagc ggacgaattt cctgaccaga
   108901 cgcacgagaa gttgtcatca caaagacgtt ttcttcacgc aagaaattga cggcgggtag
   108961 ctcgtccggc acacgaatcg gcataacctg attacctcac tacatacgct tatacgttta
   109021 gacatccaga tagctgaaga taaccagaaa atttgccaat gtcgagcctg catattgaag
   109081 gtgagaaagt ttcaaggaaa aggctacgtt agaatataag aatgacgaaa aggagagaaa
   109141 atggttatta gtattcgccg ctcacggcat gaggaagggg aggagctcgt tgcgatttgg
   109201 tgtcgttctg tcgatgccac tcacgatttt ttatcagcag cgtatcgggc cgagctggag
   109261 gagctagttc gttccttcct gccggaagcg ccgttgtggg tcgcggttaa tgagcgggat
   109321 cggccggttg ggtttatgtt gttaagtggg cagcatatgg atgcgctgtt tatcgatcct
   109381 gatgtgcgcg gctgcggcgt gggtcggatg ctggtggaac atgcgctctc gatggcaccg
   109441 gaactgacaa ccaatgttaa tgagcaaaat aagcaggctg ttgggttcta taagaagatg
   109501 ggttttaagg ttacgggacg ctctgaggtg gacgatttgg ggaaaccgta tccattgctg
   109561 aatctggcat atgtagggga ataagctgcc ccctatcgtg ctatcgtgct tgcgtaagct
   109621 ggagcagacg cgccagcgtc gcatccggca ttttttctgc gccaatgcaa aaaggccatc
   109681 ctttcggatg gccttt
//
LOCUS       DAZGQL010000002       101832 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.002, whole genome shotgun sequence.
ACCESSION   DAZGQL010000002 DAZGQL010000000
VERSION     DAZGQL010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 101832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 101832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..101832
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.002"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..182
                     /locus_tag="ACHKIR_000101"
     CDS             <1..182
                     /locus_tag="ACHKIR_000101"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9987916.1"
                     /translation="YDHPYRNDDSRFAKGIIIIVIPFPQRGKSSNCNDVRYGCLDGLS
                     GHYDHSGCTVLCLVA"
     gene            complement(271..663)
                     /locus_tag="ACHKIR_000102"
     CDS             complement(271..663)
                     /locus_tag="ACHKIR_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001694413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFO9987917.1"
                     /translation="MRDIQQVLERWGAWAANNHENVTWSSVAAGFKGIIPPKITSRPQ
                     CCDDDAMVICGCIARLYRNNRDLHDLLVDYYVLGETFMALARKHGCSDTCIGKRLHKA
                     EGVVEGMLMMLDVKLEMDRYVERDSVTA"
     gene            complement(1149..1394)
                     /locus_tag="ACHKIR_000103"
     CDS             complement(1149..1394)
                     /locus_tag="ACHKIR_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9987918.1"
                     /translation="MIEEQRERQHKQEATQPDSVTAKNYARVVAEVMRREGTELNGQD
                     CLVIRTRVLGMLAARRRQEQRQNAAPYQWHKPERLRR"
     gene            complement(1963..2394)
                     /locus_tag="ACHKIR_000104"
     CDS             complement(1963..2394)
                     /locus_tag="ACHKIR_000104"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9987919.1"
                     /translation="MKKIDSLRISKVAIISVSLLFLSACSTNNIENMPRDARVKFALM
                     RAYSDNKFDNSTVIGEVLGLSCARQLGKTLVTPLPGGSAIATSSEGDITSESDAIQDM
                     RYKAAMMGGDAIVNVVCKSGGVDWIHNCWSTVKCVGDVIKK"
     gene            2591..2977
                     /locus_tag="ACHKIR_000105"
     CDS             2591..2977
                     /locus_tag="ACHKIR_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001241336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9987920.1"
                     /translation="MRLTQYKKEILSYFTPDNLAWVRGEIGNPPFDVSGVAYLLYGMD
                     SHNNRYQLESTRRTLEAMVRDGLLERVNVYERREGTGDGVRRKVARYGLPGHSVVVRD
                     GEGRNGAIDGECIRINATDVVLVDGQ"
     gene            complement(3428..3721)
                     /locus_tag="ACHKIR_000106"
     CDS             complement(3428..3721)
                     /locus_tag="ACHKIR_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001303092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO9987921.1"
                     /translation="MITDSVAQKLEERGLWRRAATRWAEVLLHAETDSEREEAARRRA
                     ICISKTRRMPEQLVTFGDVKKAADRTLKEMGINPQDEWKNYSFSDAGDDLALS"
     gene            complement(3690..4157)
                     /locus_tag="ACHKIR_000107"
     CDS             complement(3690..4157)
                     /locus_tag="ACHKIR_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HFO9987922.1"
                     /translation="MNQQTTQQDQTTRRPTERPSWRHMTKRQRKNHRRVNRLCEYWPE
                     LFNLSEVRPLKNGVISDMLQEVRTRNIPVGKGALREAVITYTHMTRYLKALIAGGPRY
                     DLNGQPCGEVTEKEKAAASEQLMWRLEQIKQQNIQWQDTGGGESDNRQRGTET"
     gene            complement(4174..4623)
                     /locus_tag="ACHKIR_000108"
     CDS             complement(4174..4623)
                     /locus_tag="ACHKIR_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO9987923.1"
                     /translation="MTAQIAAYGRLVDDPQVKQTSKGTAMTLARMAVSLPCSQAQDGQ
                     ATLWLSVLAFGKQADALARHEKGDLISVAGNMQISQWTGQNGEIRQGWQVIADSVISA
                     RSARPGGKRGQQGKATEALERAKRQTGQQGNQPPAGGDDPFSDDIPF"
     gene            complement(5115..6938)
                     /locus_tag="ACHKIR_000109"
     CDS             complement(5115..6938)
                     /locus_tag="ACHKIR_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase-like DNA-binding domain-containing
                     protein"
                     /protein_id="HFO9987924.1"
                     /translation="MKSAPNLKKQPRGKKHADTEVIIFAGSDAWAHAKQWLEQDGKLA
                     GDNIPPVVLADEQLKDIGNLQIVPDGRKSARIYKAGHLDQVMVKGIGQKLAAAGVQDA
                     DYYPEGMHHNERQNWRNYLETERKNISDGLVIELPVKKKERGKGDDATSLALNQMGAS
                     QRGEVLLAHYGGELAINADSDTVHYYNSVVWVPVQDKELQRTMAQIFIDEDICYSQNA
                     IKSAVDTMKLSLPVMGNTARNLIGFSNGVFDTRTGDFREHDKNDWLLIASELPFTPPA
                     EGETLATHAPNFWKWLRRSVAENDRKADRVLAALFMVLANRYDWQLFIEVTGPGGSGK
                     SVMAEICTMLAGKANTVSASMKALEDARERALVVGFSLIILPDMTRYAGDGAGIKAIT
                     GGDKVAIDPKHKAPYSTRIPAVVLAVNNSAMSFSDRSGGISRRRVIFNFSEVVPENER
                     DPMLPEKIEGELAVVIRHLLTRFADQDEARRLLYEQQKSEEALAIKREGDSLVDFCGY
                     LMSSVMCDGLLVGNAEIIPFSPRRYLYHAYLSYMRAHGFSKPVTLTRFGADMPGAMAE
                     YGREYMKKRTKEGVRSNVILTDESEDWLPSCAPVKNEGDKN"
     gene            complement(6944..7057)
                     /locus_tag="ACHKIR_000110"
                     /pseudo
     CDS             complement(6944..7057)
                     /locus_tag="ACHKIR_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770167.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
     gene            complement(7124..7318)
                     /locus_tag="ACHKIR_000111"
     CDS             complement(7124..7318)
                     /locus_tag="ACHKIR_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9987925.1"
                     /translation="MCNFSDMTVDEAAKSAMQAELICSLMMGNTGEMTEGEIESLLAL
                     IKQLTGKAGSWLIAASGDMQ"
     gene            complement(7322..7507)
                     /locus_tag="ACHKIR_000112"
     CDS             complement(7322..7507)
                     /locus_tag="ACHKIR_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9987926.1"
                     /translation="MLKTFRVFSRAVNKRGHTIGITQNVKAENVQAAISTVINKSAKA
                     GLSGVIIHAVTCIQDEE"
     gene            complement(7500..7682)
                     /locus_tag="ACHKIR_000113"
                     /pseudo
     CDS             complement(7500..7682)
                     /locus_tag="ACHKIR_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536911.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
     gene            complement(7725..9317)
                     /locus_tag="ACHKIR_000114"
                     /pseudo
     CDS             complement(7725..9317)
                     /locus_tag="ACHKIR_000114"
                     /inference="COORDINATES: protein motif:HMM:NF022029.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9435..9683)
                     /locus_tag="ACHKIR_000115"
                     /pseudo
     CDS             complement(9435..9683)
                     /locus_tag="ACHKIR_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001526648.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antA/AntB antirepressor family protein"
     gene            complement(9676..10395)
                     /locus_tag="ACHKIR_000116"
     CDS             complement(9676..10395)
                     /locus_tag="ACHKIR_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFO9987927.1"
                     /translation="MKRPNMPVQGQGFVQPEISQNDILIRHKAESRIDSRVISERAGI
                     QHESIIATIKSHQSRLRELGSLPRQSVKELTYLKSGKSGRKRGRPEISYLLNELQLDY
                     LLRVIRGRDAERINQFKLDVTKAFAKHRAAEPVRREYLPGYHESRDGLKALGAERHHY
                     INLARTENRFAGLAVGERQSAGEQQLGLLIVMQKIEQAAFNEAIQNGMTPTEAVREVA
                     RRMETFASLMTSAPSLGVNHA"
     gene            complement(10461..10658)
                     /locus_tag="ACHKIR_000117"
     CDS             complement(10461..10658)
                     /locus_tag="ACHKIR_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO9987928.1"
                     /translation="MSNNLLRLSDVMRRTGYGRAWIYRLINQGRFPKPVKIGPRSVAF
                     IESEVDEWINQRIEASRNNAA"
     gene            complement(10906..11601)
                     /locus_tag="ACHKIR_000118"
     CDS             complement(10906..11601)
                     /locus_tag="ACHKIR_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001123241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9987929.1"
                     /translation="MPVVNDFPDLSHWFTVQEFTITQAALLLSGIDPLDYPTLEMVKE
                     EKHERWRMAYGFAYGMVTAIRRGVLTPVLCVAYDWGDMRRLTPAEMMDRSNNISMDGT
                     IITRYSLQEWVKNEKVDYVRPSRSISDKEIKPSNQSVIIDAESSVINSEETVLLTYGH
                     KSEGLEYVEDAIRELWATYDPDNQQTAPTRNDVVEYLKGRGAGQNMAEAVNLILRPTG
                     LQQVGRRKRKTNS"
     gene            complement(11729..12943)
                     /locus_tag="ACHKIR_000119"
     CDS             complement(11729..12943)
                     /locus_tag="ACHKIR_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001603436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO9987930.1"
                     /translation="MLLSDLAIRRAKPKEKAYTLNDGNGLSLLIEPNGSKGWRLRYRF
                     AGKPKMLSLGVYPEVSLSDARAARDEAKRLIAGGVNPSDVRKAHKREQASKFGNTFEA
                     IAREWYEKRLDRWSQSYAAEMIDTFEKDVFPYIGSKPIAEIKPMELLAVLSRLNDRGA
                     TEKLRKVRQRCGEVFRYAIITGRAEYNPAPDLASAFAPHKKEHYAYLKVDELPEFFRV
                     FNTYTGSQIVKLGMRLLILTGLRPGELRQAEWQEIDFDNRLWEVPKERMKMRRPHCVP
                     LSEQAIAILEQLKHMTGQYPFIFPGRIQHSKPMSEMAMNVLIRRIGYAGRVTGHGFRH
                     TMSTILHEQGYNSAWIETQLAHVDKNSIRGTYNHAQYLDGRREMLQWYADYMDSLEQG
                     GNVVHGKFGKCG"
     gene            complement(13108..13181)
                     /locus_tag="ACHKIR_000120"
     tRNA            complement(13108..13181)
                     /locus_tag="ACHKIR_000120"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13147..13149),aa:Gly,seq:ccc)
     gene            complement(13260..14012)
                     /gene="actS"
                     /locus_tag="ACHKIR_000121"
     CDS             complement(13260..14012)
                     /gene="actS"
                     /locus_tag="ACHKIR_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase activator ActS"
                     /protein_id="HFO9987931.1"
                     /translation="MSAGRLNKKSLGIVMLLSIGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKNSSTRKSTAKSTTK
                     TASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYAA
                     GAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGSTD
                     AASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            14428..16725
                     /gene="xdhA"
                     /locus_tag="ACHKIR_000122"
     CDS             14428..16725
                     /gene="xdhA"
                     /locus_tag="ACHKIR_000122"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HFO9987932.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAISINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALADDAAPIHNGGN
                     LLKQSSMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALNIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            16736..17614
                     /gene="xdhB"
                     /locus_tag="ACHKIR_000123"
     CDS             16736..17614
                     /gene="xdhB"
                     /locus_tag="ACHKIR_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HFO9987933.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPVTQRHLPALCAAASSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCQLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            17611..18090
                     /gene="xdhC"
                     /locus_tag="ACHKIR_000124"
     CDS             17611..18090
                     /gene="xdhC"
                     /locus_tag="ACHKIR_000124"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HFO9987934.1"
                     /translation="MNHSETITIECTINGIPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITGIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(18130..19908)
                     /gene="ygeV"
                     /locus_tag="ACHKIR_000125"
     CDS             complement(18130..19908)
                     /gene="ygeV"
                     /locus_tag="ACHKIR_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFO9987935.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQLEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDENRVQFVNQTALKTLGVVQNNIIGKPVRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFISEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVSEAHLALDDAGGTALEEMEKQMIREALSRHNSK
                     KEVADELGIGIATLYRKIKKYELLNT"
     gene            20387..21574
                     /gene="ygeW"
                     /locus_tag="ACHKIR_000126"
     CDS             20387..21574
                     /gene="ygeW"
                     /locus_tag="ACHKIR_000126"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HFO9987936.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEERTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
     gene            21632..22828
                     /gene="dpaL"
                     /locus_tag="ACHKIR_000127"
     CDS             21632..22828
                     /gene="dpaL"
                     /locus_tag="ACHKIR_000127"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HFO9987937.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYHP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQRQSLMEKLALNKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            22886..24097
                     /gene="ygeY"
                     /locus_tag="ACHKIR_000128"
     CDS             22886..24097
                     /gene="ygeY"
                     /locus_tag="ACHKIR_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HFO9987938.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQVYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            24150..25535
                     /gene="hyuA"
                     /locus_tag="ACHKIR_000129"
     CDS             24150..25535
                     /gene="hyuA"
                     /locus_tag="ACHKIR_000129"
                     /EC_number="3.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HFO9987939.1"
                     /translation="MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGSNISPQVPYEEI
                     DATGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRITPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            25583..26515
                     /gene="arcC"
                     /locus_tag="ACHKIR_000130"
     CDS             25583..26515
                     /gene="arcC"
                     /locus_tag="ACHKIR_000130"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFO9987940.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVEAGQVVITVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(26556..28181)
                     /gene="yqeB"
                     /locus_tag="ACHKIR_000131"
     CDS             complement(26556..28181)
                     /gene="yqeB"
                     /locus_tag="ACHKIR_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HFO9987941.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EALDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILQVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTECGFIPVIVDPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTGVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(28229..28999)
                     /gene="yqeC"
                     /locus_tag="ACHKIR_000132"
     CDS             complement(28229..28999)
                     /gene="yqeC"
                     /locus_tag="ACHKIR_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HFO9987942.1"
                     /translation="MKSIIDPSALFIDLGAQKRPTVISVVGAGGKTSLLFWLAELFQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASFISPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRSECDVILIEADGSRGMPLKAPDEHEPCIPKSSCCVIAVMGGHILGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            29103..29681
                     /gene="mocA"
                     /locus_tag="ACHKIR_000133"
     CDS             29103..29681
                     /gene="mocA"
                     /locus_tag="ACHKIR_000133"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HFO9987943.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKIMLPWQQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIQRPNVTNMRQALLMG
                     EHYSVEIENAEIILDIDTPDDFITAKKRYTEI"
     gene            30003..33101
                     /gene="ygfK"
                     /locus_tag="ACHKIR_000134"
     CDS             30003..33101
                     /gene="ygfK"
                     /locus_tag="ACHKIR_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HFO9987944.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMFQPSDSCKSFIFNMSVGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDALNKLLQDDAFLARHGLQEKRESLQALPARIPTS
                     MVQGVTLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMALAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWGLDHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQFRIPPELIQHDIDFVAAHGVKFEYGCSPD
                     LTVEQLKNQGFHYVLIATGTDKNSGVKLAGDNQNVWKSLPFLREYNKGTELKLGKHVV
                     VVGAGNTAMDCARAALRVPGVEKATVVYRRSLQEMPAWREEYEEALHDGVEFRFLNNP
                     ERFDADGTLTLRVMSLGEPDEKGRRRPVETNETVTLHVDSLITAIGEQQDTEALNAMG
                     VPLDKNGWPDVDHNGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDAILSRENIRSH
                     QNDKYWNNVNPAEIYQRKGDISVTLVDSDDRDAFVAQEAARCLECNYVCSKCVDVCPN
                     RANVSIAVPGFQNRFQTLHLDAYCNECGNCAQFCPWNGKPYKDKITVFSLSQDFDNSS
                     NPGFLVEDCRVRVRLNNQSWVLNIDNEGQFNNVPPELNDMCRIISHVHQHHHYLLGRV
                     EV"
     gene            33104..34432
                     /gene="ssnA"
                     /locus_tag="ACHKIR_000135"
     CDS             33104..34432
                     /gene="ssnA"
                     /locus_tag="ACHKIR_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HFO9987945.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSGLSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKGGCTSVIDHHASPAYIDGSLSTLRNAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARQIDDAKKAATEPYLVEAHIGAHAPFTVPDAGLEMLREAVK
                     ATGRGLHIHAAEDLYDVSHSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDIALL
                     NQRDAFLVHNARSNMNNHVGYNHHLCDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALANGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            34483..35262
                     /gene="ygfM"
                     /locus_tag="ACHKIR_000136"
     CDS             34483..35262
                     /gene="ygfM"
                     /locus_tag="ACHKIR_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HFO9987946.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQTLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEEYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAITDHDGMRIALDGVASKALRLHDVEKQNLEGDALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            35259..38129
                     /gene="xdhD"
                     /locus_tag="ACHKIR_000137"
     CDS             35259..38129
                     /gene="xdhD"
                     /locus_tag="ACHKIR_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HFO9987947.1"
                     /translation="MIIHFTLNGTPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIDAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLEVIGKHYPKTDAAKMVQAKPCYVEDRVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEDIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEEDNC
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKRDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNGDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEPVADVQLATPGVVRGKKGEVSFGEIAHKGETGTGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYDAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDACGIWLREWHFTPEKILTALEKI"
     gene            38294..39694
                     /gene="xanQ"
                     /locus_tag="ACHKIR_000138"
     CDS             38294..39694
                     /gene="xanQ"
                     /locus_tag="ACHKIR_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanQ"
                     /protein_id="HFO9987948.1"
                     /translation="MSDINHAGSDLIFELEDRPPFHQALVGAITHLLAIFVPMVTPAL
                     IVGATLQLSAETTAYLVSMAMIASGIGTWLQVNRYGIVGSGLLSIQSVNFSFVTVMIA
                     LGSSMKSDGFHEELIMSSLLGVSFVGAFLVVGSSFILPYLRRVITPTVSGIVVLMIGL
                     SLIKVGIIDFGGGFAAKSSGTFGNYEHLGVGLLVLIVVIGFNCCRSPLLRMGGIAIGL
                     CVGYIASLCLGMVDFSSMRNLPLITIPHPFKYGFSFSFHQFLVVGTIYLLSVLEAVGD
                     ITATAMVSRRPIQGEEYQSRLKGGVLADGLVSVIASAVGSLPLTTFAQNNGVIQMTGV
                     ASRYVGRTIAVMLVILGLFPMIGGFFTTIPSAVLGGAMTLMFSMIAIAGIRIIITNGL
                     KRRETLIVATSLGLGLGVSYDPEIFKILPASIYVLVENPICAGGLTAILLNIILPGGY
                     RQENVLPGITSAEEMD"
     gene            39712..41028
                     /gene="guaD"
                     /locus_tag="ACHKIR_000139"
     CDS             39712..41028
                     /gene="guaD"
                     /locus_tag="ACHKIR_000139"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008892 - guanine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006147 - guanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HFO9987949.1"
                     /translation="MSGEHTLKAVRGSFIDVTRTVDNPEEIASALRFIEDGLLLIKQG
                     KVEWFGEWEDGKHQIPDTIRVRDYRGKLIVPGFVDTHIHYPQSEMVGAYGEQLLEWLN
                     KHTFPTERRYEDLEYAREMSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHINM
                     RMIAGKVMMDRNAPDYLLDTAESSYHQSKELIERWHKNGRLLYAITPRFAPTSSPEQM
                     AMAQRLKEEYPDTWVHTHLCENKDEIAWVKSLYPDHDGYLDVYHQYGLTGKNCVFAHC
                     VHLEEKEWDRLSETKSSIAFCPTSNLYLGSGLFNLKKAWQKKVKVGMGTDIGAGTTFN
                     MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGNFLPGKEADFVVMEP
                     TATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYERN"
     gene            41064..42431
                     /gene="ghxQ"
                     /locus_tag="ACHKIR_000140"
                     /gene_synonym="ygfQ"
     CDS             41064..42431
                     /gene="ghxQ"
                     /locus_tag="ACHKIR_000140"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
                     /protein_id="HFO9987950.1"
                     /translation="MSGDILQTPDAPKPQGALDNYFKITARGSTVRQEVLAGLTTFLA
                     MVYSVIVVPGMLGKAGFPPAAVFVATCLVAGFGSLLMGLWANLPMAIGCAISLTAFTA
                     FSLVLGQQISVPVALGAVFLMGVIFTAISVTGVRTWILRNLPMGIAHGTGIGIGLFLL
                     LIAANGVGMVIKNPIEGLPVALGAFTSFPVMMSLLGLAVIFGLEKCRVPGGILLVIIA
                     ISIIGLIFDPAVKYHGLVAMPSLTGEDGKSLIFSLDIMGALQPTVLPSVLALVMTAVF
                     DATGTIRAVAGQANLLDKDNQIINGGKALTSDSVSSIFSGLVGAAPAAVYIESAAGTA
                     AGGKTGLTATVVGALFLLILFLSPLSFLIPGYATAPALMYVGLLMLSNVSKLDFNDFI
                     DAMAGLVCAVFIVLTCNIVTGIMLGFVTLVVGRVFAREWQKLNIGTVIITAALVAFYA
                     GGWAI"
     gene            complement(42467..42955)
                     /gene="ygfS"
                     /locus_tag="ACHKIR_000141"
     CDS             complement(42467..42955)
                     /gene="ygfS"
                     /locus_tag="ACHKIR_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFO9987951.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADVFLPRLKVQ
                     RLDSISAPVMCHQCENAPCVSACPVGALTMGEQVVQTNSARCIGCQSCVSACPFGMIT
                     IQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRQQRIVASGEN
                     PL"
     gene            complement(42955..44874)
                     /gene="ygfT"
                     /locus_tag="ACHKIR_000142"
     CDS             complement(42955..44874)
                     /gene="ygfT"
                     /locus_tag="ACHKIR_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HFO9987952.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAMAHNQENWPLSHSDFRPRIHV
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQPDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQQIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKTHFGEIYYGLDPQQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRIEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
     gene            45309..46757
                     /gene="uacT"
                     /locus_tag="ACHKIR_000143"
     CDS             45309..46757
                     /gene="uacT"
                     /locus_tag="ACHKIR_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HFO9987953.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKEAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            46759..46884
                     /gene="yqfG"
                     /locus_tag="ACHKIR_000144"
     CDS             46759..46884
                     /gene="yqfG"
                     /locus_tag="ACHKIR_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqfG"
                     /protein_id="HFO9987954.1"
                     /translation="MNLLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(46881..46952)
                     /locus_tag="ACHKIR_000145"
     CDS             complement(46881..46952)
                     /locus_tag="ACHKIR_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9987955.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            47007..47555
                     /gene="idi"
                     /locus_tag="ACHKIR_000146"
     CDS             47007..47555
                     /gene="idi"
                     /locus_tag="ACHKIR_000146"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFO9987956.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEDAVIRRCRYELGVEITPPESIYPDF
                     YYRATDPNGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQAANSEARKLLSAFAQHN"
     gene            complement(47598..49115)
                     /gene="lysS"
                     /locus_tag="ACHKIR_000147"
     CDS             complement(47598..49115)
                     /gene="lysS"
                     /locus_tag="ACHKIR_000147"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFO9987957.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(49125..50223)
                     /gene="prfB"
                     /locus_tag="ACHKIR_000148"
     CDS             complement(join(49125..50147,50149..50223))
                     /gene="prfB"
                     /locus_tag="ACHKIR_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFO9987958.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            complement(50314..52047)
                     /gene="recJ"
                     /locus_tag="ACHKIR_000149"
     CDS             complement(50314..52047)
                     /gene="recJ"
                     /locus_tag="ACHKIR_000149"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFO9987959.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHSGVEHARSLGIPVIVTD
                     HHLPGETLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGVA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAL
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQIEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLTYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            complement(52053..52763)
                     /gene="dsbC"
                     /locus_tag="ACHKIR_000150"
     CDS             complement(52053..52763)
                     /gene="dsbC"
                     /locus_tag="ACHKIR_000150"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HFO9987960.1"
                     /translation="MKKGFMLFTLLAAFSGFVQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKDMKEFLDEHQKMTSGK"
     gene            complement(52788..53684)
                     /gene="xerD"
                     /locus_tag="ACHKIR_000151"
     CDS             complement(52788..53684)
                     /gene="xerD"
                     /locus_tag="ACHKIR_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFO9987961.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            53796..54317
                     /gene="fldB"
                     /locus_tag="ACHKIR_000152"
     CDS             53796..54317
                     /gene="fldB"
                     /locus_tag="ACHKIR_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HFO9987962.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            complement(54357..54764)
                     /gene="ygfX"
                     /locus_tag="ACHKIR_000153"
     CDS             complement(54357..54764)
                     /gene="ygfX"
                     /locus_tag="ACHKIR_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HFO9987963.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     SGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(54745..55011)
                     /gene="sdhE"
                     /locus_tag="ACHKIR_000154"
     CDS             complement(54745..55011)
                     /gene="sdhE"
                     /locus_tag="ACHKIR_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HFO9987964.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            55264..56244
                     /gene="ygfZ"
                     /locus_tag="ACHKIR_000155"
     CDS             55264..56244
                     /gene="ygfZ"
                     /locus_tag="ACHKIR_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HFO9987965.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMTEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANIFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLTGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            complement(56321..56980)
                     /gene="yqfA"
                     /locus_tag="ACHKIR_000156"
     CDS             complement(56321..56980)
                     /gene="yqfA"
                     /locus_tag="ACHKIR_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFO9987966.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     IKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(57144..57455)
                     /gene="yqfB"
                     /locus_tag="ACHKIR_000157"
     CDS             complement(57144..57455)
                     /gene="yqfB"
                     /locus_tag="ACHKIR_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-acetylcytidine aminohydrolase"
                     /protein_id="HFO9987967.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTDLKKVIADIYPGQTQFYVIEFKC
                     L"
     gene            57500..58933
                     /gene="bglA"
                     /locus_tag="ACHKIR_000158"
     CDS             57500..58933
                     /gene="bglA"
                     /locus_tag="ACHKIR_000158"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HFO9987968.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDDMFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLHEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQKPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGEKL"
     gene            complement(59097..61970)
                     /gene="gcvP"
                     /locus_tag="ACHKIR_000159"
     CDS             complement(59097..61970)
                     /gene="gcvP"
                     /locus_tag="ACHKIR_000159"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HFO9987969.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLARAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLDID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            complement(62089..62478)
                     /gene="gcvH"
                     /locus_tag="ACHKIR_000160"
     CDS             complement(62089..62478)
                     /gene="gcvH"
                     /locus_tag="ACHKIR_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HFO9987970.1"
                     /translation="MSNVPAELKYSKEHEWLRKEAGGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            complement(62502..63596)
                     /gene="gcvT"
                     /locus_tag="ACHKIR_000161"
     CDS             complement(62502..63596)
                     /gene="gcvT"
                     /locus_tag="ACHKIR_000161"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HFO9987971.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYSQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            63887..63970
                     /locus_tag="ACHKIR_000162"
     CDS             63887..63970
                     /locus_tag="ACHKIR_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9987972.1"
                     /translation="MSKNTKSKIHNIRKYNAKTEVKLVYFK"
     gene            complement(64044..65246)
                     /gene="ubiI"
                     /locus_tag="ACHKIR_000163"
                     /gene_synonym="visC"
     CDS             complement(64044..65246)
                     /gene="ubiI"
                     /locus_tag="ACHKIR_000163"
                     /gene_synonym="visC"
                     /EC_number="1.14.13.240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HFO9987973.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQAGEDEFNRALNIAFDNRLGLCKVESERLVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(65269..66447)
                     /gene="ubiH"
                     /locus_tag="ACHKIR_000164"
                     /gene_synonym="visB"
     CDS             complement(65269..66447)
                     /gene="ubiH"
                     /locus_tag="ACHKIR_000164"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HFO9987974.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGCVLVA
                     ADGTHSALATVCGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQYGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTRAARP
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAQERGEDMGDYGVLCRYQQ
                     RRQRDREATIGVTDSLVHLFANRWGPLVVGRNIGLMAMELFTPARDVLAQRTLGWVAR
                     "
     gene            complement(66444..67769)
                     /gene="pepP"
                     /locus_tag="ACHKIR_000165"
     CDS             complement(66444..67769)
                     /gene="pepP"
                     /locus_tag="ACHKIR_000165"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HFO9987975.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYTYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITALAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENECELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSMQEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            complement(67795..68373)
                     /gene="ygfB"
                     /locus_tag="ACHKIR_000166"
     CDS             complement(67795..68373)
                     /gene="ygfB"
                     /locus_tag="ACHKIR_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HFO9987976.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            68541..68870
                     /gene="zapA"
                     /locus_tag="ACHKIR_000167"
     CDS             68541..68870
                     /gene="zapA"
                     /locus_tag="ACHKIR_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFO9987977.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALFEQGRITE
                     KTNQNFE"
     gene            68912..69095
                     /gene="ssrS"
                     /locus_tag="ACHKIR_000168"
     ncRNA           68912..69095
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHKIR_000168"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            69116..69718
                     /gene="fau"
                     /locus_tag="ACHKIR_000169"
     CDS             69116..69718
                     /gene="fau"
                     /locus_tag="ACHKIR_000169"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFO9987978.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTLPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKMQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(69819..69878)
                     /gene="ibsC"
                     /locus_tag="ACHKIR_000170"
     CDS             complement(69819..69878)
                     /gene="ibsC"
                     /locus_tag="ACHKIR_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HFO9987979.1"
                     /translation="MMRLVIILIVLLLISFPAY"
     gene            complement(70107..71339)
                     /gene="serA"
                     /locus_tag="ACHKIR_000171"
     CDS             complement(70107..71339)
                     /gene="serA"
                     /locus_tag="ACHKIR_000171"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFO9987980.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLAEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            complement(71595..72254)
                     /gene="rpiA"
                     /locus_tag="ACHKIR_000172"
     CDS             complement(71595..72254)
                     /gene="rpiA"
                     /locus_tag="ACHKIR_000172"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFO9987981.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            complement(72310..72495)
                     /locus_tag="ACHKIR_000173"
     CDS             complement(72310..72495)
                     /locus_tag="ACHKIR_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001328908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9987982.1"
                     /translation="MHFAQRVRALVVLNGVALLPLFASWSGIPRPLYALFAGRKGMPA
                     IARYFMDELTTRLANGV"
     gene            72636..73529
                     /gene="argP"
                     /locus_tag="ACHKIR_000174"
     CDS             72636..73529
                     /gene="argP"
                     /locus_tag="ACHKIR_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HFO9987983.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            73733..75877
                     /gene="scpA"
                     /locus_tag="ACHKIR_000175"
     CDS             73733..75877
                     /gene="scpA"
                     /locus_tag="ACHKIR_000175"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HFO9987984.1"
                     /translation="MSKVQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSFF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCRTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEAAFDRYLVPSQCVTGVIAQSYHQSEKSAAE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGRKDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            75870..76865
                     /gene="meaB"
                     /locus_tag="ACHKIR_000176"
     CDS             75870..76865
                     /gene="meaB"
                     /locus_tag="ACHKIR_000176"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HFO9987985.1"
                     /translation="MINEAKLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLIVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNRLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            76876..77661
                     /gene="scpB"
                     /locus_tag="ACHKIR_000177"
     CDS             76876..77661
                     /gene="scpB"
                     /locus_tag="ACHKIR_000177"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HFO9987986.1"
                     /translation="MSYQYVNVVTINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            77685..79163
                     /gene="scpC"
                     /locus_tag="ACHKIR_000178"
     CDS             77685..79163
                     /gene="scpC"
                     /locus_tag="ACHKIR_000178"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HFO9987987.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPNAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(79160..80056)
                     /gene="ygfI"
                     /locus_tag="ACHKIR_000179"
     CDS             complement(79160..80056)
                     /gene="ygfI"
                     /locus_tag="ACHKIR_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO9987988.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATMLQHIAEDRDGTSH"
     gene            complement(80224..80964)
                     /gene="yggE"
                     /locus_tag="ACHKIR_000180"
     CDS             complement(80224..80964)
                     /gene="yggE"
                     /locus_tag="ACHKIR_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HFO9987989.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            complement(81057..81692)
                     /gene="argO"
                     /locus_tag="ACHKIR_000181"
     CDS             complement(81057..81692)
                     /gene="argO"
                     /locus_tag="ACHKIR_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0061459 - L-arginine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HFO9987990.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVAFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIVHAQALLS"
     gene            complement(81831..82691)
                     /gene="mscS"
                     /locus_tag="ACHKIR_000182"
     CDS             complement(81831..82691)
                     /gene="mscS"
                     /locus_tag="ACHKIR_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HFO9987991.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTDIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            complement(82882..83961)
                     /gene="fbaA"
                     /locus_tag="ACHKIR_000183"
     CDS             complement(82882..83961)
                     /gene="fbaA"
                     /locus_tag="ACHKIR_000183"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFO9987992.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            complement(84175..85338)
                     /gene="pgk"
                     /locus_tag="ACHKIR_000184"
     CDS             complement(84175..85338)
                     /gene="pgk"
                     /locus_tag="ACHKIR_000184"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFO9987993.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            complement(85388..86407)
                     /gene="epd"
                     /locus_tag="ACHKIR_000185"
     CDS             complement(85388..86407)
                     /gene="epd"
                     /locus_tag="ACHKIR_000185"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048001 - erythrose-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HFO9987994.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            complement(86692..87405)
                     /gene="yggC"
                     /locus_tag="ACHKIR_000186"
     CDS             complement(86692..87405)
                     /gene="yggC"
                     /locus_tag="ACHKIR_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HFO9987995.1"
                     /translation="MKIELTVNGLKIQAQYQNEEIENVHKPLLHMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFNVAKLAENLCRVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WQELASFCDFSIFIHAPAQILRERLISRKIAGGLSRQEAEAFYARTDGPNVERVLVNS
                     RQANLILEMTEEGRYHFKS"
     gene            complement(87402..87911)
                     /gene="fumE"
                     /locus_tag="ACHKIR_000187"
     CDS             complement(87402..87911)
                     /gene="fumE"
                     /locus_tag="ACHKIR_000187"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HFO9987996.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQFWHYLNEQD
                     ETPGFADDMTWDFISNVNSITCNATLYDALKAMKFADFAVWSEARFSRMVKTALTLAV
                     TTTLKELTP"
     gene            complement(87933..88248)
                     /locus_tag="ACHKIR_000188"
                     /pseudo
     CDS             complement(87933..88248)
                     /locus_tag="ACHKIR_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class II"
     gene            complement(88245..89522)
                     /gene="yggP"
                     /locus_tag="ACHKIR_000189"
     CDS             complement(88245..89522)
                     /gene="yggP"
                     /locus_tag="ACHKIR_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFO9987997.1"
                     /translation="MKTKVAAIYGKRDVRLREFELPEITDNELLVSVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVIVEVGKNLTDKYKKGQRFVLQPAMGLPSG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQV
                     QKLLPVELAASKGIELVYMNTKGMSDPVQTLRALTDDAGFDDVFVYAAVPSVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQL
                     QPSFMVTHIGGLDAVPDTVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKE
                     LARLVEETHGIWNEQAEKYLLAQFGVDIGEAAQ"
     gene            complement(89537..90925)
                     /gene="cmtA"
                     /locus_tag="ACHKIR_000190"
     CDS             complement(89537..90925)
                     /gene="cmtA"
                     /locus_tag="ACHKIR_000190"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HFO9987998.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYIDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     FVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWMFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVGTLVSFAITSVI
                     LKMEKTVETESEDEFAQSANAVKAMKQEGAFSLSRVKRIAFVCDAGMGSSAMGATTFR
                     KRLEKAGLAIEVKHYAIENVPADADIVVTHASLEGRVKRVTDKPLILINNYIGDPKLD
                     TLFNQLTAEHKN"
     gene            complement(90953..91396)
                     /gene="cmtB"
                     /locus_tag="ACHKIR_000191"
     CDS             complement(90953..91396)
                     /gene="cmtB"
                     /locus_tag="ACHKIR_000191"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HFO9987999.1"
                     /translation="MRLIDYFPESSISVIHSAKDWQEAIDFSMASLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLKQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILNQLLTAASEKQLADIISRG"
     gene            91773..92681
                     /locus_tag="ACHKIR_000192"
     CDS             91773..92681
                     /locus_tag="ACHKIR_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HFO9988000.1"
                     /translation="MKAPVSITSFRPQKSIAIVIGVLAVVVLPFLSYYTVNEGERGIL
                     LRYGKIVKVAEPGLGFKIPFMESVEKISTRNQAVVYQGLQAYSRDQQPAQMTVSVSFH
                     IKPSEAGAVYTTYNTIEALKDRLIVRQLPTQLENVFGQYTAISAVQDRTKLVQDLQNA
                     MRKAVVGPVVIDGVQIENIDFSDAYEKSIEDRMKAEVAIATRKQNLETEKIQAQIAVT
                     QAQAEADSKLAAAKAEAETIRVRGAAEAETIRLKSAAEAEAIRLRGEALRDNPGLVAL
                     TTAERWDGKLPDTMIPGSTVPFISTK"
     gene            complement(92727..94718)
                     /gene="tkt"
                     /locus_tag="ACHKIR_000193"
     CDS             complement(92727..94718)
                     /gene="tkt"
                     /locus_tag="ACHKIR_000193"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFO9988001.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAEQLFEEFGFTVDNVVAKAKALL"
     gene            94996..95754
                     /gene="loiP"
                     /locus_tag="ACHKIR_000194"
                     /gene_synonym="yggG"
     CDS             94996..95754
                     /gene="loiP"
                     /locus_tag="ACHKIR_000194"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HFO9988002.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGVK"
     gene            complement(95802..96722)
                     /gene="speB"
                     /locus_tag="ACHKIR_000195"
     CDS             complement(95802..96722)
                     /gene="speB"
                     /locus_tag="ACHKIR_000195"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HFO9988003.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(96858..97589)
                     /locus_tag="ACHKIR_000196"
     CDS             complement(96858..97589)
                     /locus_tag="ACHKIR_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9988004.1"
                     /translation="MKKWKVRSALIALAVLLAGCSSNAQYNSSASGNVGTAWGGDVHS
                     SVQGVSAERVWRDPAEMIVISYSTTVPSGYDRVYSIRINELEYAIRDGNFNSLPITRV
                     YDSSNNEPRYIVHARVGMNYQLYIRNYSRNTNYEIVATVDGLDVLNGKQGSLNNNGYI
                     VNAGDSLAIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGPAQ
                     NALPPCSGQAFPADNNGYAPPPCRK"
     gene            complement(97735..99711)
                     /gene="speA"
                     /locus_tag="ACHKIR_000197"
     CDS             complement(97735..99711)
                     /gene="speA"
                     /locus_tag="ACHKIR_000197"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HFO9988005.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(99720..99851)
                     /gene="yqgB"
                     /locus_tag="ACHKIR_000198"
     CDS             complement(99720..99851)
                     /gene="yqgB"
                     /locus_tag="ACHKIR_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HFO9988006.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            complement(99945..100088)
                     /gene="yqgG"
                     /locus_tag="ACHKIR_000199"
     CDS             complement(99945..100088)
                     /gene="yqgG"
                     /locus_tag="ACHKIR_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgG"
                     /protein_id="HFO9988007.1"
                     /translation="MNRCLLLNLSHRSGEDSFPALCISALHTCRCYTHLGASQDSRAG
                     YSY"
     gene            complement(100199..100450)
                     /gene="yqgD"
                     /locus_tag="ACHKIR_000200"
     CDS             complement(100199..100450)
                     /gene="yqgD"
                     /locus_tag="ACHKIR_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgD"
                     /protein_id="HFO9988008.1"
                     /translation="MVADPTTTLQVKNTGSLSVNRYGWINIWMAILGQFFTRFPLFFE
                     SCLILLKTWLEIFPDNAGILRIYLLQFSAIVGYKTRRAA"
     gene            100506..101660
                     /gene="metK"
                     /locus_tag="ACHKIR_000201"
     CDS             100506..101660
                     /gene="metK"
                     /locus_tag="ACHKIR_000201"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFO9988009.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
BASE COUNT        25024 a        26849 c        24987 g        24972 t
ORIGIN      
        1 attatgacca tccctaccgt aatgatgatt cacgctttgc taaaggtatc atcattatcg
       61 taataccttt tccccaacgg ggtaaaagct ccaactgtaa cgatgttcgt tatggttgcc
      121 tcgatggtct gagtggtcat tatgaccatt cagggtgcac cgtcctttgt ctcgtggcat
      181 aattatcagg cgattacgtt tttcatgatt gattggcaag ccgtatttct ccaccgcagt
      241 ttcctcatga ctgcggtttt tttatgcgcg ttatgccgtc actgaatcgc gctcaacata
      301 ccgatccatc tccagcttca catccagcat catcaacatg ccttcaacaa ccccctccgc
      361 tttgtgaagg cgtttaccta tacaggtgtc agagcaccca tgtttccgtg ccagcgccat
      421 gaacgtctcc cccaacacgt aataatcaac cagcaagtca tgcagatcgc gattgttccg
      481 gtaaaggcgg gctatgcacc cgcatatcac catcgcatca tcgtcacagc actgcggacg
      541 agatgttatc tttggcggaa taatcccctt aaatccggca gcaacggatg accaggtcac
      601 attctcatgg ttatttgccg cccacgctcc ccatcgctca agcacctgtt gaatatcgcg
      661 cacgtgttac gccccctgtg atgtgtttaa ggttgtgcta tgacttatgc cgattgctgt
      721 aaggcccacc agccgcgtac atcactcgat tgtgagggat gtaaaaatca gggctggctg
      781 gtccggtctg gatggtttac ataccccgat atcggggatg tacaaatcat ggttttacga
      841 ctggtgaatt tgaacaactc aaataatcga gtaaatgagc aaaaaacatc aatgggttag
      901 ggtaatacca cgcagcagtg tttctcaact cccaccattg acggaagttg aataaacggg
      961 taagtccaaa gctcaaaaat gagctttgta acaatatcag caagttaccg cgccagcttc
     1021 caccaatggt ggattgcgga agaatcaatg tattaggcca aagtgcaaat ttgcactctg
     1081 gaacaatatc aacaagttaa cgcagtcagc tacccggctc agaattgagc tgcctttaaa
     1141 tcaacaagtt accgccgtaa ccgctccggc ttatgccact ggtacggggc cgcgttctgt
     1201 cgctgctcct gccgcctcct tgccgccagc ataccaagca ccctggtacg gataaccaag
     1261 cagtcctgcc cgttcagttc agtgccttca cggcgcatca cttccgcaac aacacgcgca
     1321 taatttttag cggtcacgct gtccggctgc gtggcctcct gtttgtgctg gcgttctctc
     1381 tgctcttcaa tcatgaggat taactcgcgt tcctcctgct cttcaataat cccgtgaatt
     1441 atctctgttg gtgaacgttc ggtcatcggg cctcctgtgt gtcactgttt tggtgtcaat
     1501 gtcactgtta cggcgacacc gtattttgcc caaatcaggc aaaattgctt acccgaatcc
     1561 ggtaaatttg cgcgcttttt cgatcatcgc gtatcggttt cgttttgcgc ttcctgttgc
     1621 gtttcgtttt tgagaaaaat gcgcactttt tccggtggcc tttcccccct gcaagccgcg
     1681 tcatacctgg cttttctctg ctggtctgtg tgatcggttt tatgatcgat tttgtgatcg
     1741 gttttatgat cgatttgcgc agttttttgc gcaattttct gcgcattttt tcagggattt
     1801 ttcaccccat cggaggggct tccgaggggg ggctgtgaag ggggtagcat tttgctgtga
     1861 tggaagaggg caattgtaac aggggtagct aacggtaagc tgcaatcaca cgtcgtaatt
     1921 gcagtttcca tgtattaatc aataccttca gtatggcaac tgttattttt ttattacatc
     1981 accaacgcac ttaacagttg accaacagtt atgaatccag tcaacaccac ctgatttaca
     2041 tacaacgttc actatggcat cacctcccat cattgcggct ttatatcgca tgtcctgtat
     2101 tgcatccgac tcagaagtaa tgtcgccctc agaggatgta gcaatagcgc ttccccctgg
     2161 caatggtgtt actaatgttt ttccaagttg tctggcgcaa ctcaatccta acacttcgcc
     2221 gataactgtt gaattatcaa acttgttatc tgaataagcc ctcattagag caaattttac
     2281 acgcgcatct ctcggcatat tctcaatatt atttgtagaa cacgcgctaa gaaataataa
     2341 agatactgat ataatagcta ctttacttat tcgtaaagaa tcaatttttt tcatcttgcg
     2401 tacctcacaa cacaacaatt tgaatattca tatttgcctc atattgccgc aacccatcgc
     2461 ataacccttg tgcttccagc gcttcagcct tgtcatcacg tatcatctgt accccgccga
     2521 ttctgtcctt tcaaaatttg ccttatggct tattgtatga gggattcatc ctacaacaag
     2581 aaaggtcact atgagactga cgcaatacaa aaaagagatc ctcagttact tcactcccga
     2641 caatctggcg tgggtcagag gtgaaattgg taacccgccg tttgatgtct ccggtgtggc
     2701 ttacctgctg tatggcatgg attcacacaa caaccgatat cagctcgaat caacaaggcg
     2761 cacacttgaa gcgatggtcc gtgacggtct tctggagcgg gttaacgtgt atgagcggcg
     2821 tgagggaaca ggtgatggtg ttcgtcgaaa ggtggcgcgt tatggcctgc ctggtcattc
     2881 tgttgtggtg cgtgacggtg agggccggaa cggtgcaatt gacggcgaat gtatacgcat
     2941 caatgctaca gatgtggtgc tggtggacgg gcaataaaaa aacctgaata actccgctgg
     3001 gtgcttcagt tgcacggagc gaaatcattc agggtcagcc gtaacgggtc tctgtacagg
     3061 tggcttcagt tgccagaaga tgagcgcccg ttacagcctg ctgtgataag ccgctttaca
     3121 gtgagcttca attgctcaaa gaggcgcgtt tttatcacaa ctttgccgca ataacccgct
     3181 gtacagaggg ctccagttgc ccaaagaggc gcattcttat cgcggcatga cgaaatcacc
     3241 gtgatgtttt cgtcgttcac tcatcccgat gtgtgtgaca agtacacatc aaaatgagtg
     3301 ccgaaaagcc gctggctggc ttcattcggc gaggctcccg ttgcctgcgg agaacaccat
     3361 caacggccgt tcggtggtga ttatagcgaa tcgcgataat tgagcaaata cggcgatgat
     3421 atgcgtatca cgatagcgct aaatcgtcgc ctgcatcact gaagctgtaa tttttccatt
     3481 catcctgcgg attaatcccc atctctttca gcgttctgtc tgcggccttt ttcacatcgc
     3541 caaaggtgac cagttgttca ggcatacgac gggttttact gatgcatatc gcccgacgtc
     3601 tggcagcttc ctcgcgttca ctgtccgttt ctgcgtgaag aagaacctca gcccatcggg
     3661 tcgccgcacg tcgccataaa ccacgctcct caagtttctg tgccacgctg tctgttatca
     3721 ctctcaccac ctcccgtatc ctgccactgg atgttttgct gttttatctg ctccagcctc
     3781 cacatcagtt gttcgctggc tgctgctttc tctttctccg tgacttcgcc gcaaggttgc
     3841 ccgttcaggt cgtaccgtgg tccaccagca atcagcgcct taaggtatcg cgtcatgtgg
     3901 gtgtaagtga ttaccgcctc acgtaatgca cctttgccta caggtatgtt tcttgtcctg
     3961 acctcctgca acatatcgct gattacgccg ttcttgaggg gcctgacttc cgagaggtta
     4021 aacagttcag gccagtactc gcacagacgg tttacgcgtc tgtggttttt tcgctgccgc
     4081 ttcgtcatgt gtcgccatga tgggcgctcc gtgggtctgc gagtggtctg gtcctgttgt
     4141 gtggtctgct ggttcatgtt gtgctcctgt cattcagaaa ggaatatcgt cagagaacgg
     4201 gtcatcacct cccgctggtg gctgatttcc ctgctgtccg gtctgtcgtt ttgctctttc
     4261 cagtgcttcg gtcgccttcc cctgttgccc ccgcttcccg ccaggacgcg ccgaacgggc
     4321 actgattacg ctgtcagcga taacctgcca gccctgccgt atttctccgt tctgtccggt
     4381 ccactggctg atctgcatgt tacccgccac gctgataagg tcaccttttt catgtctcgc
     4441 cagcgcgtcg gcctgcttac caaatgccag gactgatagc cataacgtcg cctgtccgtc
     4501 ctgtgcctga ctgcatggca gtgataccgc catacgcgcc agcgtcatcg ctgttccctt
     4561 gctggtctgt tttacctgcg ggtcgtccac cagccgcccg taagctgcaa tttgtgctgt
     4621 catggtctgc gctccttgtt ccggtactcc tggataatcc ccattgtcat atcgaataaa
     4681 tcacatgccg ctttttctgc atcttccgct ttcaggtcat acagcgtcat gaaatggtga
     4741 gcgattgccc gtgcggatct tttgctgatc cctgttcggt taaatcgatg gcgtgtgagt
     4801 gtgatttttt agcgtgtttc tctgtcataa aatttttctc agaggaataa ataaaaattt
     4861 atacccaaaa ggtgttcaac tgttcacctt ttgctatttc acctttagat tcataaagtt
     4921 aaggggtgaa cactttttgt aaaggtgttc accaactgtt caccctgttc acccgcgact
     4981 gttcaaaaat catacaaaaa ggtgaacagg tgaatacttt ggtgaatagt tttacaaaag
     5041 tgttcaccca ttaacatatt gatactaaat acatttattg cagggtgaac agtggtgaac
     5101 agtttttcta taacttaatt tttatcaccc tcatttttca ctggtgcaca tgacggcagc
     5161 cagtcctcag actcatccgt cagtatcacg tttgatcgca caccttcttt agttctcttt
     5221 ttcatatact ccctgccata ttccgccatt gcgcctggca tatctgcacc gaagcgcgtc
     5281 agtgttacgg gcttactgaa accgtgcgcc ctcatatacg agagataggc gtgataaaga
     5341 taacggcgtg gactgaaagg aataatctca gcattaccga ctaacaggcc gtcacacatt
     5401 accgacgaca tgagatagcc gcagaagtcc accagcgaat ccccctcacg ctttatcgcc
     5461 agtgcttctt ctgatttctg ctgctcatat aaaaggcgtc tggcttcgtc ctgatcagca
     5521 aaccgtgtaa gcagatgacg aatcactaca gcaagctcac cttctatttt ttccggcagc
     5581 atcggatcgc gttcgttttc cggtacaacc tccgaaaaat tgaatatcac acgacgacgt
     5641 gagatccccc cgctgcggtc actgaatgac atggcgctgt tgttaaccgc cagcacaacc
     5701 gccggaatac gcgttgagta tggcgctttg tgtttcgggt caattgccac cttgtcaccg
     5761 cccgtaatgg cctttatccc tgcaccatca cccgcgtagc gggtcatatc cggcagaata
     5821 atcagtgaaa agccaaccac taatgcacgt tcccttgcat cctccagtgc cttcatgctt
     5881 gccgataccg tgttggcctt acctgccagc atggtacaaa tctctgccat aacactttta
     5941 ccgcttccac caggacctgt tacctcaatg aataactgcc agtcgtaccg gttcgccagc
     6001 accataaata acgctgccag aacgcgatct gccttgcggt cattctcagc caccgaacgg
     6061 cgcaaccatt tccagaaatt cggcgcatgt gttgccagcg tttcaccctc tgctggtggg
     6121 gtgaacggta attcactggc aattaacagc cagtcatttt tatcgtgttc acggaaatca
     6181 cctgtacggg tatcaaatac cccgttactg aatccaatca ggttgcgggc ggtattcccc
     6241 attacgggta aacttaactt catggtatca accgccgatt ttatggcgtt ttgtgaatag
     6301 cagatatctt cgtcaatgaa gatttgcgcc attgtgcgct gtagctcttt atcctgtacg
     6361 ggaacccata caacactgtt gtaataatgc actgtgtcag agtcggcgtt tattgccagt
     6421 tcgccgccat aatgtgccag aagaacttcg ccgcgctggc ttgcccccat ctggtttagc
     6481 gccagtgatg tggcatcatc accttttccc cgctcttttt tctttacagg cagctcaata
     6541 accagcccgt cagaaatgtt tttgcgctca gtctccagat agttacgcca gttctgccgc
     6601 tcgttatggt gcataccttc ggggtaatag tctgcgtcct gtacgcctgc cgccgccagc
     6661 ttctgaccta tgcctttgac catcacctga tcgaggtgtc cggccttgta tatacgcgct
     6721 gatttgcgcc cgtcgggtac aatttgcaga ttgccaatat ctttaagttg ctcatcagcc
     6781 agcacgacgg gcggtatatt atctccggca agttttccgt cctgctccag ccactgtttc
     6841 gcgtgcgccc acgcatcact gccagcgaaa ataatcactt ccgtatcggc gtgttttttc
     6901 cctcgaggct gtttttttaa gttcggtgcg cttttcattt cttacctctg ctgatcatcg
     6961 tcatgtggct accgtattca ttgcggtaaa tatccccgca cataaattcg gggtgagttt
     7021 tgctactggc agttaagcca gaatatttac gtttcatcgt tttatttcct gatatgtaac
     7081 gctttaattc gctggtattt atttcatcct gttatttatt cccttattgc atatcacccg
     7141 atgccgcaat cagccagcta ccagccttac ctgtaagctg cttaatcagg gcgagcaatg
     7201 actcaatctc accctcagtc atttcacctg tgttacccat catcagagaa caaataagtt
     7261 ctgcctgcat tgcgcttttt gctgcttcat caacagtcat atcggaaaaa ttacacatga
     7321 attactcctc atcctgaata catgtcactg cgtggataat tacgccggat aaaccggctt
     7381 tagctgattt gttaattact gtgctgattg ctgcctggac gttttcggcc ttcacgttct
     7441 gtgtgatgcc gatggtatgg ccgcgcttgt taacagcacg ggaaaagacg cggaaggttt
     7501 taagcatgac tcaccccctg acgaatacgg gcgacaaaaa tcagatgtgc gtgcggcagc
     7561 cgggcgcgtg cttcgcgctc agtggcagcg gtaacggtga atattgcaat gctgcctgtc
     7621 aggcactgca taaagcgcca gacaaagtaa gggcgtgcgg atacagccat gtgaaaggct
     7681 cctctgactt tgggattata cccaccgccg gaggtggtaa gctcgctggc ggtggactgt
     7741 acagggttac cacaactggc gttagaggaa accagcccga ccgaagtcgg ccccatacag
     7801 cccaccattg ttgaggtgtg cgtgtgcgcc gatacaaaaa aagacgctgg cgcgtcgtgt
     7861 atcgcctcta actcctgcgg ggtggtaatc ccgatccccg ttttataagg gatagcctca
     7921 gtatatgccg cttttttgct taagtggtcg gatgtgttgc gagaaaagtt tgtgcgaaaa
     7981 atattcacat atccttccag aaaggatgga taccagaaag aaagacaacg taccagacaa
     8041 taaccagcgc cgtgatatga ggcatcacga cataatgcat taccggaatc cagtatgagt
     8101 aagcacagat tttcgcccgt taccccgaat atcagcgcac tacaacgcca gcgactgata
     8161 cgtgacatca ttgaaaccag gaaagaaaaa ttaaccccgc cagaaaaaac cagcgaaccg
     8221 ccaccgcttg aggtgtttct cagtggcaga aaaaaccaac gcaataaatt acaacaacag
     8281 aaaccatcat cgctgtagcg tggtgtgtta cggcgtattg ctttacaggt acagacaatg
     8341 acaaatgaag aactacagga aatcgcatta tccatacttg agcgacagcg cgaaaaagca
     8401 caacggcaaa gaaggttgtt acttaatcca cagccactca ccaggccaac acgatttgcc
     8461 cttgaggctt ttgtcgagaa atacccacgg cggttaaaag ccactaaaaa ccgaccgcca
     8521 ggctgatagc tcgctgtttc ttctgcggct ctatgaaaca gacagaaacc atcagcgccg
     8581 tgatgtgagg aagtacggcg cattgtttta caggtgcaca atatggataa cacacaacac
     8641 acttcacaat cagagaataa aaaagatgat ctcacagcat atgagcaaca tttcgttgaa
     8701 aaaaaacagt caataacgag acacatcgca gatatggata tgcgcaagcg atttgaaaaa
     8761 actttcaata ccaattttgc gcttgaggat tttttaaagc agcattccag ccgatcaagc
     8821 aataagggat agcttatgga tgaccagaaa aaaaactcct ttgccatgat ggttacactg
     8881 accagcgccc gtataactga acatgaacga aagcggaaag ctgcacgcga acaggcaaga
     8941 gcaacagaat taacacgtaa agaacttaac gctctggcac gggaacacag agaagcacct
     9001 gaagcttttg cacttgataa ttttctgaaa agtacatgcc gtaagaaaat acgaacatcg
     9061 ggaccaaggg gccattcgtc gtaaactgca tattgtccgc cagcctcaga tatggcattg
     9121 tggataatgc gcttgatttc ctttatctcc actggcgacc ctttcgtggt cgcctttgtt
     9181 ttgcctgcca ttcctgcatc atgcggatat tcgcgccgta cagggccacc agcgcgataa
     9241 tttgataaac cctcccccaa gtattgctta acgtgtttca tgcgctcagc ggtgaaatgg
     9301 ctcacgatat ggcgcatgta atcagcatgg ttgatgctca cctgtgcgaa cttatccgca
     9361 gtatcgatgt acagggcggc agcgttgcgg atgtcaggta tgttcacgtt ctcccaggcg
     9421 acgtgccagc cagaatcaaa ctgttcaata tttacacttt ggctcccccg tttcggggaa
     9481 tcaaagtaca aattttctac aacctcaaaa tccacacctt ttttaaatcc gtactcctca
     9541 atacgccctt tgatccaggt ggtgaaatca cggcctacac cgagcgcttt atgcagtgcg
     9601 cgtgcgctga caacgcccgt ttcatatctg ccaataatgc caggtgatac cggaataatt
     9661 gcggcgaaat cataattacg catgattcac ccccagagac ggcgctgacg tcatcaggct
     9721 ggcgaacgtc tccatgcggc gagcaacttc ccgcaccgct tcggtcggtg tcatgccgtt
     9781 ctgtatcgcc tcgttaaatg ctgcctgctc gattttctgc atgactatca gtaagcctaa
     9841 ctgttgctct cccgcgctct gacgctcgcc tacagccagc ccagcgaagc gattttcagt
     9901 gcgggcaagg ttgatgtaat ggtgacgttc tgcgcccagt gctttaagcc cgtcacggct
     9961 ttcgtgatag cctggcagat attcgcgacg gactggttca gcggcgcgat gcttcgcgaa
    10021 tgccttcgta acatccagct tgaactggtt gatacgttct gcgtcacgcc cacggataac
    10081 gcgtaacaga tagtcaagct gtagctcatt cagcaaataa ctaatttcag gacgaccgcg
    10141 cttgcggcca gattttcccg atttcaaata ggttaattcc ttgactgact ggcgaggcag
    10201 tgagccaagc tcgcgcaatc tggattgatg agatttaatc gtcgcaatga tactttcgtg
    10261 ttgtattcct gctcgctcag aaataacgcg ggaatcaata cgcgactccg ctttgtggcg
    10321 aatcaatata tcgttctggc tgatttcagg ctgaacgaag ccctgcccct gtacgggcat
    10381 attcggacgt ttcattgaaa aactcctgct atcggatcag gtttagtgct gggggagaat
    10441 atccccctcc caaaagatta ttacgcggcg ttgttgcgag aagcctcgat acgctggtta
    10501 atccattcat cgacttcgct ttcaataaaa gccacggagc ggggaccaat ttttacaggt
    10561 ttaggaaagc gcccctggtt gatcaggcga taaatccaag cgcgaccgta accagtacgg
    10621 cgcataacat cagaaagacg aagtaagtta tttgacatat tcaatcctcg taacgtctat
    10681 aaccgtctac aatgatgcat aaaaggaaaa catatttata ggtggatact atttttaatg
    10741 gtgggtacta tttcaatgga atagtgccca ctattatttt aataatgctg ccaccattcc
    10801 agtatcaata agcaaagaaa acgcacgctt aactatgatt tctcgccata aacaaaagag
    10861 ttttgagcaa atagtgccac cttttttacg aagggtgggc actatttaac tatttgtttt
    10921 tcttttcctt cttccaactt gctgcaagcc agtggggcgt agtatcaggt ttacagcctc
    10981 ggccatgttc tgccccgctc ccctgccttt caggtattca acaacatcat ttcttgtagg
    11041 ggctgtttgt tgattatctg gatcatatgt tgcccaaagt tctcttattg cgtcttctac
    11101 atattcaaga ccttcgctct tatgaccata agtcagcaat actgtttctt cagaattaat
    11161 cacgctagat tcggcatcaa ttattactga ttggttagat ggcttaatct ccttatccga
    11221 tatagagcga gagggtctta cataatcaac tttctcattt tttacccatt cctgtaatga
    11281 ataacgggta ataattgttc cgtccatgga tatattattt gaacgatcca tcatctctgc
    11341 tggggttaaa cgacgcatat ccccccaatc atacgcaacg cacagaacgg gagttaagac
    11401 accgcgtcga attgcagtaa ccattccata agcaaaccca tatgccattc tccatcgttc
    11461 gtgtttttcc tctttgacca tctccagagt tgggtagtcg agaggatcaa tacctgacag
    11521 caataacgca gcttgagtaa tagtaaactc ctgcactgta aaccaatgac ttaagtcagg
    11581 gaaatcattc acaaccggca tgtgcacacc tcgcactctc tgtagtggtg gctgtgccag
    11641 tccgcagagg tgtacggggt ttcgggagcg accctagaca cagcctgtta tttagtcgct
    11701 caaagtctac tactgtatac tcaaccagtc atccacattt tccaaacttg ccatgtacca
    11761 cgtttccgcc ctgttccagt gagtccatat agtcggcgta ccactggagc atctcccgcc
    11821 gtccgtccag atattgcgcg tgattgtatg tgccacggat cgagttttta tcgacgtgcg
    11881 ccagctgcgt ttctatccat gcggagttat acccctgctc gtgcaaaatc gtactcatgg
    11941 tgtgccggaa cccatgacca gtaacacgcc ctgcataacc aatacgacga ataagcacgt
    12001 tcatcgccat ttcactcatc ggtttactgt gctgtattcg accagggaaa ataaacggat
    12061 actgccccgt catgtgtttc agttgttcca gaatagctat cgcctgttct gaaagcggca
    12121 cgcaatgagg acggcgcatt ttcattcgtt ctttcggcac ttcccacaga cgattatcaa
    12181 aatcaatctc ctgccactct gcctgccgca attcacctgg gcggagtcct gtaagaatca
    12241 ataatcgcat acccagcttg acgatctggc ttccggtgta cgtattaaaa acgcggaaaa
    12301 attccggtag ctcatcaact ttcagatacg cgtaatgctc ttttttgtgt ggtgcaaatg
    12361 cgcttgccag atcaggagcg gggttatatt cagcgcgtcc ggtaattatt gcgtaccgga
    12421 acacttcgcc acatcgctgg cgaaccttac gcaatttttc agttgcgcca cggtcattaa
    12481 gacgggagag aaccgcgagc aattccatcg gttttatttc tgctatcggt ttgctgccga
    12541 tatacgggaa tacgtccttc tcaaacgtat caatcatctc tgcggcatat gattgtgacc
    12601 atctgtccag gcgtttttca taccactcac gggcgatcgc ctcaaaggtg ttaccgaatt
    12661 tgctcgcctg ttctctctta tgggcttttc tcacatcact tggattaact ccaccagcga
    12721 taagcctttt ggcttcatcc cttgccgccc tggcatcaga aagggacacc tcaggataaa
    12781 cacccagcga caacattttg ggcttacctg caaacctgta tcgcaatcgc cagcccttcg
    12841 aaccgttagg ctcaatgagt aacgaaagcc cgttgccgtc attgagtgta taggctttct
    12901 cttttggctt cgcgcggcgg atagcaaggt ctgaaagcaa catgtgtata gcccccaaaa
    12961 aacgattttg tcctatacgc attactatac acttctgtgt gtagatttag gtagatgact
    13021 gtagacgctt gtagacgagg aaaatcgctg gaggccttga tattcggggg attggcagac
    13081 tttagtagac gttggtagat gtttgtttgg agcgggcgaa gggaatcgaa ccctcgtata
    13141 gagcttggga agctctcgtt ctaccattga actacgcccg cttcgagatg cgtaaggcat
    13201 tataaacctt acgctctcct tagcaagtgc cgcgctgctg actgctgatt aattcgccat
    13261 cagcattttg gcttgctgcc ctgaggcggc aagtaacgta gcggatcaat tgccgttgca
    13321 cggtaacgaa tctggaaatg caggcgaacc gatgccgcat cagtgctccc catagtggcg
    13381 attttttgcc cagccttcac gctttgccca ttatttacca gcatcgtgtc attatgggcg
    13441 taagccgtaa tgtaatcttc actgtgttta atcatgatga gattaccgta gccacgcagc
    13501 tggttgccca catacaccac ctttcctgca cccgcggcgt aaataggtgt accccgtgga
    13561 gctgagatat caatcccttt attgccgcca tcagctgtcg aatacggcat gataactttc
    13621 cctgtcgttg gccataacca acaacgttgc cctactggcg gccaggaaga tttcggtact
    13681 gctgatgacg gtgtaaccga tgcggttttg gtcgttgatt tggcggttga tttacgtgta
    13741 ctactatttt tcgccccacc cagttttagt ttctgaccaa cttcaatggt gtaagggggg
    13801 gaaatgccgt tcagtcgcgc cagctctttt acgctagttc ccgtggtgcg cgaaatacga
    13861 tatagcgtat ccccccgttt cacggtgtaa acggagccgg aatacgttcc tgtatcggat
    13921 gatttgctac ccgagcagcc cgccaaaagc agtccaatcg ataacaacat cacgataccc
    13981 agagattttt tattcaggcg tcccgcactc aaaatctttc ctcgcttaaa aaacaagacg
    14041 ccatatcata gcagccagcc tcttgacacc caatctcttg cttctggaaa aacgcggtaa
    14101 tagagaaaga ggttaatttc atttggcaaa gggaagtatc atcagcaaaa tattcactgt
    14161 agatgtatcg aattttctga ctcatttacc ctatttccca tcctgatgcg taataatccc
    14221 aatttatcac attgatttta tcgccactca tattgatacg tatcactgcc cctattcagg
    14281 gtcctgtttc gtatcatatc tgtgtttttt taacatcatc acacttcctt cctcccttaa
    14341 cgcttatcgt cgtttctggc gtaaatcttg cctgcttaga ctaaatcttt gccataagaa
    14401 ccaaatgttc aaatcgaggg gatttctatg gaagcgcggg aagcaaccgc tacaggtgaa
    14461 tcatgcatgc gcgtagatgc cattgctaag gtcaccgggc gggcacgata tactgacgat
    14521 tatgttatgg cgggcatgtg ttacgcgaaa tatgtacgta gccctatcgc acatggttat
    14581 gccataagta ttaatgatga acaagccaga agtttaccgg gcgtactggc gatttttacc
    14641 tgggaagatg tgcctgatat cccattcgct acagctgggc atgcctggac acttgacgaa
    14701 aacaagcgcg ataccgccga tcgcgcactg ctaactcgcc atgttcgtca tcatggtgac
    14761 gccgttgcca tcgtcgtggc ccgtgatgaa ctcacggcag aaaaagcggc gcaattggtc
    14821 agcattgagt ggcaagaatt acccgttatc accacgccag aagcggcgct ggcagatgac
    14881 gctgcaccaa tccataacgg cggcaattta ctaaaacaaa gctcgatgtc gacgggtaat
    14941 gtccaacaaa caatcgatgc cgccgattac caggtgcagg ggcactatca gactcccgtt
    15001 attcaacatt gtcatatgga aagcgtgaca tcgctggcat ggatggagga tgactcgcga
    15061 attaccatcg tttccagcac ccagatcccg cacattgttc gccgtgtggt tggtcaggcg
    15121 ctgaatattc cctggtcatg cgtacgagtc atcaaaccgt ttgtcggtgg cggttttggt
    15181 aataaacagg atgtactgga agagccaatg gcggcattcc tgaccagcaa gcttggcggc
    15241 attccggtga aagtttccct tagccgtgaa gagtgtttcc tcgcaacccg tacccgccac
    15301 gcttttacta ttgacgggca aatgggcgtg aaccgcgacg gaacattgaa aggttatagt
    15361 ctggatgttc tgtctaacac cggcgcttat gcatctcacg ggcactccat cgcttctgcg
    15421 ggcggaaata aagtcgctta cctttatcct cgttgtgcct acgcttacag ttcaaagacc
    15481 tgctatacca acctcccctc ggctggtgcg atgcgtggtt atggcgcgcc acaagtcgta
    15541 tttgccgttg agtctatgct tgatgacgcc gcgacagcgt taggtattga tcctgttgaa
    15601 attcgtttac gcaacgccgc ccgcgaagga gatgctaatc cgctcacggg caaacgtatt
    15661 tacagcgcag ggttgccgga gtgtcttgaa aaaggccgga aaatctttga atgggaaaaa
    15721 cgccgtgcag agtgccagaa ccagcaaggc aatttacgcc gcggcgttgg cgtcgcctgt
    15781 tttagctaca cctctaacac ctggcctgtc ggcgtagaaa tagcaggcgc gcgcctgttg
    15841 atgaatcagg atggaaccat caacgtgcaa agcggcgcga cagaaatcgg tcagggggcc
    15901 gacaccgtct tctcgcaaat ggtagcagaa accgtggggg ttccggtcag cgacgttcgc
    15961 gttatttcaa cccaagatac cgacgttacg ccgttcgatc ccggtgcatt tgcctcacgc
    16021 cagagctatg ttgccgcgcc tgcgctgcgc agtgcggcac tgttattaaa ggagaaaatc
    16081 atcgctcatg ccgctgtcat gctacatcag tcagcgatga atcttaccct gataaaaggc
    16141 catatcgtgc tggttgaacg accggaagag ccattaatgt cgttaaaaga tttggcgatg
    16201 gacgccttct accaccctga acgcggcggg cagctctctg ccgaaagctc catcaaaacc
    16261 accactaacc caccagcgtt tggctgtacc tttgttgatc tgacggtcga tattgcgctg
    16321 tgtaaagtca ccatcaaccg catcctcaac gttcatgatt cgggccatat tcttaatccg
    16381 ctactggcag aaggtcaggt acacggcgga atgggaatgg gcattggctg ggcgctattt
    16441 gaagagatga tcattgatgc gaaaagcggc gtggtccgta accccaatct gctggattac
    16501 aaaatgccga ccatgccgga tctgccacaa ctggaaagcg cgttcgtcga aatcaatgag
    16561 ccgcaatccg catacggaca taagtcactg ggtgagccac caataattcc tgttgccgct
    16621 gctattcgta acgcggtgaa gatggctacc ggtgttgcaa tcaatacact gccgctgacg
    16681 ccaaaacggt tatatgaaga gttccatctg gcaggattga tttgaggata acatcatgtt
    16741 tgattttgct tcttaccatc gcgcagcaac ccttgccgat gccatcaacc tgctggctga
    16801 caacccgcag gccaaactgc tcgccggtgg cactgacgta ctgattcagc tccaccatca
    16861 caatgaccgt tatcgccata ttgttgatat tcataatctg gcggagctgc ggggaattac
    16921 gctggcggaa gatggctcac tacgtatcgg ctctgcaacg acatttaccc agctcattga
    16981 agatcctgta actcaacgtc atctcccggc gttatgtgct gcggcctcat ccattgccgg
    17041 accgcagatc cgtaacgtcg ctacctacgg tggaaatatt tgcaacggtg ccaccagcgc
    17101 agattctgcc acgccaacgc taatttatga cgcgaaactg gagatccact ccccgcgcgg
    17161 tgttcgtttc gtcccgatta atggctttca caccgggccg ggcaaagtgt ctcttgagca
    17221 tgacgaaatc cttgtcgcct ttcattttcc gccccagccg aaagaacacg cgggcagcgc
    17281 gcattttaaa tatgccatgc gcgacgcaat ggatatttca acgattggct gcgccgcaca
    17341 ttgccaactg gataacggca atttcagcga attacgcctg gcatttggtg ttgccgcgcc
    17401 aacgccaatt cgctgccaac atgccgaaca gactgcacaa aatgcgccat taaacctgca
    17461 aacgctggaa gccatcagcg aatcagtcct gcaagatgtc gccccgcgtt cgtcatggcg
    17521 ggccagtaaa gagtttcgtc tgcatctcat ccagacgatg accaaaaaag tgattagcga
    17581 agccgtcgcc gcggcggggg gaaaattgca atgaatcaca gcgaaacaat taccatcgaa
    17641 tgcaccatta acgggatacc ttttcagctt cacgccgcac caggcacgcc gctctcggaa
    17701 ttactccgcg aacaaggact gctaagtgtc aaacaagggt gctgcgtggg tgaatgtggt
    17761 gcctgtacgg tgctggtcga cggcacagca atagacagtt gcttatacct tgccgcctgg
    17821 gctgaaggaa aagagatccg cacgctggaa ggtgaagcga aaggcggaaa actttctcat
    17881 gttcagcagg cttatgcgaa atccggcgca gtgcagtgcg ggttttgtac gccgggcctg
    17941 attatggcta ccacggcaat gctggcgaaa ccacgcgaga agccattaac cattacggga
    18001 attcgtcgtg gactggcggg aaatctttgt cgctgcacgg ggtatcagat gattgtaaat
    18061 acagttctgg attgcgagaa aacgaagtaa aaggatatcc ggcctgaatt caggccggat
    18121 tcactgaggt tatgtgttta acaactcata tttcttaatc ttgcgataga gcgtagcaat
    18181 gccgatgccc agttcatcag caacttcctt cttgctgtta tgacgtgaaa gcgcctcgcg
    18241 gatcatttgc ttttccatct cctccagcgc cgtgccgccc gcatcatcga gtgccaggtg
    18301 cgcctcactg acctctgtta catcactttg ctccgttgtg ccattattca gcagatttgg
    18361 cggcaatagc gtgctgtcga taacttcacc tgaaggaacc acgttaacca gatattccat
    18421 caaattgctt aactcgcgca ggtttccggg ccaacgatgc ttacgcaata tttcgacgac
    18481 atcgggagca atgcccggat aaaccgatcc cagacgacgg gtatgcagat gtaaaaagta
    18541 atgcaccagt agttcaatat cttcctgacg ttcacgcagc ggtggcagag ttatcgggat
    18601 aacattaagt cggtagaaga gatcttcgcg gaatttacct tcggaaatga actgggccaa
    18661 attctggtta gttgcagaaa tgatgcgaat gtcgacttgt attgggctac tggcaccaat
    18721 cggcagaatt tcacgtgcct caatagcgcg cagtaattta gcctgcaaca ttaatggcat
    18781 atcacctatt tcatcgagaa acagcgtgcc cgtattcgcc gcctgaatca accctgtttt
    18841 accgttggca gaagcgccag taaatgcgcc tttaacataa ccgaacagtt cgctctccag
    18901 aagctgctcc ggaatcgcgg cacagttgat agcaataaaa ggtttattcc gtcttccgct
    18961 caacttatgg attgcacggg cgacgacttc tttacccgtg ccgctttcgc caaccaccat
    19021 aacgctggat gggctgggtg caatacggct aatgagtcgt tttaattgcc gcataacacg
    19081 gcactcgcca accaattgtt caatatgcgg ttcatcaggt gcatttgcta cagaaaaact
    19141 ggtatgcgat tggtgaaacg ccattaaaaa taattgtcgg ccctgaatgt tatgcaattg
    19201 accaatgatt aattcacttt tatcgtccca cgaaacaata tgctgcatat gtccatgggt
    19261 aaaattactc tcaaatgtta atggtctgaa acgaacaggt ttcccaataa tattattttg
    19321 cacaacaccg agtgttttta aggcagtctg attaacaaac tgaacccgat tttcgtcatc
    19381 aacaactaaa acgccctgat ccatattatc gatcatggtc gcgaatattt tactgatgtt
    19441 atctcctggc ccctgatcct ccagaagttt cgaaacaaaa atggtggata tatggcgaac
    19501 gtaatcagaa aattcgcgta aattatcact gatatgttct tgttgctcgt gggtaacggc
    19561 aatcaaactt atcaccccaa cacaacgatc ctgtaaaatg acaggcgtac ccagaaatgc
    19621 tttttcgcgg caattttctt tactatcgca accttcacaa aggggatcga agcgagactg
    19681 tgtcacaact ttttcagttt tcgtttccag gacgtggcgg agcaggcgtg agttgccgct
    19741 caactggcga ccaagaaact tcccatacgc gcccgttccg gcgacgcgac acaagttttc
    19801 atcaacgatc tcaacctcaa gctgcaaaac gctggcaagc attctggcaa aacgctgaat
    19861 tgtcggttga atttgcatca atactgactg cgtagtagca agctccatag ctttaccttc
    19921 cagacttact taaaagtcga tcattgaaga cgttgatggt tcatagatca tgatgatatt
    19981 aactcaggcg aaattggctt tgataaaaac ataagatttt tatcactttc taatgaaatt
    20041 atggaagaga tatcacattt ctatatcaat atgagaatta cggcggtgag tttatcaaac
    20101 tgaagagaga tcgcctgtcc ctttatctta tttctgatac ttagcagcaa ataaataacg
    20161 cgataaaaaa agccaaacgt tttcgtattt tacaaacaac cagaagctgg catcaatttg
    20221 tgatcaaccc cacacattat ccgtcaaatt agtcttttgc agccccgtgg ataattctgg
    20281 cacacttatt gttagtccca ggtatagctg tgaaaacacc aatcacgttg gcaagtcaca
    20341 gtgaaataaa ccactttgcc tgtcattcca ctaccgggac tttatgatga aaactgttaa
    20401 tgagctgatt aaggatatca attcgctgac ctctcacctt cacgagaaag attttttgtt
    20461 aacgtgggaa cagacgccag atgaactgaa acaagtactg gacgttgccg cagcattaaa
    20521 agcactgcgt gctgaaaaca tctcaaccaa agtctttaat agtggattag gtatttccgt
    20581 attccgcgac aactccaccc gtacccgctt ctcttatgct tccgcgctta acctgctcgg
    20641 ccttgcacag caagatctcg acgaaggcaa atcacaaatc gctcacggcg aaaccgtgcg
    20701 tgaaaccgcc aatatgatct ccttctgcgc cgatgccatc ggtattcgcg acgatatgta
    20761 tctgggcgca ggcaacgcct atatgcgtga agttggcgcg gcacttgatg acggctacaa
    20821 acaaggggtt ctgccacagc gtccggcttt agtgaacctg caatgcgata ttgaccaccc
    20881 gactcagtct atggctgacc tggcatggtt gcgtgaacac tttggttcgc tggaaaacct
    20941 gaaaggcaaa aaaatcgcca tgacctgggc ctactcccca agctatggca aaccgctctc
    21001 tgtaccacaa ggcatcatcg gtctgatgac ccgcttcggt atggatgtca ccctggccca
    21061 tccggaaggc tacgacctga tcccggatgt cgttgaagtt gctaaaaaca atgctaaagc
    21121 ctccggtggt agcttccgtc aggtcaccag catggaagaa gccttcaaag acgcagacat
    21181 cgtttatccg aagtcatggg caccttacaa agtgatggaa gagcgtactg aattgctgcg
    21241 tgcgaacgat cacgaaggct taaaagcact ggaaaaacag tgtctggcac agaacgcgca
    21301 acacaaagac tggcattgta ctgaagagat gatggaactg acccgtgatg gcgaagccct
    21361 gtacatgcac tgcctgccag ctgatatcag cggcgtatcc tgcaaagaag gtgaagtgac
    21421 tgaaggcgta ttcgaaaaat accgtatcgc tacctacaaa gaagccagct ggaagcctta
    21481 tatcatcgcc gcgatgatcc tgtcccgtaa atacgccaaa ccaggtgcac tgctcgagca
    21541 actgctgaaa gaagcgcaag aacgcgtgaa ataacccctc gggccgatca gctggtcggc
    21601 cctctatttc cagaggccaa aaggatagga tatgtccgtt ttctcattga agattgatat
    21661 cgccgataac aaatttttca acggcgaaac atcaccgctt ttttcgcaaa gccaggcaaa
    21721 actggcacgc cagttccacc agaaaattgc tggttatcac ccaacaccgc tttgtgcgct
    21781 ggacgatctc gcaaacctgt ttggtgtaaa gaaaattctc gtcaaagacg aatcaaaacg
    21841 attcggtctg aacgccttca aaatgcttgg cggtgcgtac gccatcgctc aattattgtg
    21901 tgaaaaatat catcttgata tcgaaacgct gtcatttgag cacctgaaaa atgccatcgg
    21961 cgaaaaaatg actttcgcga ctaccaccga cggcaaccac gggcgcggtg tggcatgggc
    22021 agcacagcaa ctcggacaga atgcggtgat ttacatgccg aaaggttctg ctcaggaacg
    22081 cgttgacgcc attctaaacc tcggtgccga gtgcatcgtc acggatatga actatgacga
    22141 taccgttcgc ctgaccatgc aacacgcgca gcagcacggc tgggaagtgg tacaggacac
    22201 ggcatgggaa ggttacacca aaatcccaac ctggatcatg caaggctacg caacactggc
    22261 ggatgaagcc gtcgagcaaa tgcgtgaaat gggcgtcacc ccgacacacg ttctgctgca
    22321 agccggtgtc ggtgcaatgg ccggtggtgt gctgggttat ctggtcgacg tctatagccc
    22381 gcaaaatctg cacagcatta ttgttgaacc tgacaaagct gactgtattt atcgctccgg
    22441 cgtcaaaggc gacatcgtca acgttggcgg tgatatggcc accatcatgg cgggcctggc
    22501 ctgcggcgaa cctaacccac tgggctggga aatcctacgt aactgcgcca cccaattcat
    22561 ctcctgccag gacagcgttg ccgcattagg tatgcgcgtg ctgggtaatc cgtatggcaa
    22621 cgacccgcgc atcatctccg gtgaatccgg cgctgtcggt ttgggcgttc tcgcagcggt
    22681 tcattatcac ccgcaacgtc aaagcctgat ggaaaaactg gcgctgaaca aagatgccgt
    22741 ggtgctggtt atcagcactg aaggcgacac cgacgtgaag cactaccgcg aagttgtctg
    22801 ggaaggcaaa cacgctgtag caccttaatc accctattgg aactgctccc tgcaaaacgg
    22861 ggagtaaaaa atctggagaa aaataatggc taagaatatt ccattcaaac tgattcttga
    22921 aaaagcaaaa gattaccagg cagacatgac tcgcttcctg cgcgatatgg ttgctattcc
    22981 cagtgaaagc tgcgacgaga aacgcgtagt acatcgtatt aaagaagaga tggaaaaagt
    23041 cggcttcgat aaagttgaaa tcgacccgat gggcaacgtt ctcggttata tcggccacgg
    23101 cccgcgtctg gtggcaatgg acgctcatat cgataccgtc ggcattggca acatcaaaaa
    23161 ctgggacttc gatccgtacg aaggcatgga aactgatgag ctgatcggtg gtcgcggtac
    23221 ttccgaccag gaaggcggca tggcatctat ggtttatgcc ggtaaaatca ttaaagacct
    23281 cggtctggaa gatgaatata ccctgctggt taccggtact gtgcaggaag aagactgcga
    23341 cggcctgtgc tggcagtaca ttattgaaca atccggcatt cgcccggaat ttgtggtcag
    23401 taccgaacca accgactgcc aggtataccg tggtcagcgc ggtcgtatgg aaattcgtat
    23461 tgatgttcag ggtgtgagct gccacggttc tgcaccagaa cgcggtgaca acgccatttt
    23521 caaaatgggt ccgattcttg gcgaattaca agaactctcc caacgtctgg gttatgacga
    23581 attcctcggc aaaggcaccc tcaccgtttc tgaaatcttc ttcacatccc caagccgttg
    23641 cgctgtagca gatagctgcg ccgtctctat tgaccgccgt ctgacctggg gcgaaacctg
    23701 ggaaggcgcg ctggacgaaa tccgcgccct gcctgcagta cagaaagcta acgcggttgt
    23761 ttctatgtac aactacgacc gtccgtcctg gactggcctg gtttacccaa ccgaatgcta
    23821 cttcccgacc tggaaagtgg aagaagatca cttcaccgtt aaagcactgg tgaatgccta
    23881 cgaaggtctg tttggcaaag cgccggttgt tgataagtgg accttctcaa ctaacggcgt
    23941 atctatcatg ggccgtcacg gcattccggt gatcggcttt ggcccgggta aagaacctga
    24001 agcgcatgca cctaacgaaa aaacctggaa atctcacctg gtgacctgtg ccgcgatgta
    24061 cgctgcaatc ccgttaagct ggctggcaac cgaataatta ctcttcactt attcccctcc
    24121 ggttcgccgg agggtttttg gagtttgcta tgcgcgtatt gatcaaaaac ggcactgtcg
    24181 ttaacgcaga tggacaagcc aaacaagatt tgctgattga gagcgggatt gttcgccaat
    24241 tgggcagcaa tatttcgccg caggtcccgt atgaagaaat tgatgccact ggctgttacg
    24301 ttttccctgg cggcgtggat gtccatacgc atttcaatat tgatgtcggc atcgcgcgca
    24361 gttgtgatga tttttttacc ggtacccgcg cagctgcgtg tggcggtaca acaaccatta
    24421 ttgaccatat gggatttggc ccgaatggct gccggttacg ccatcaactg gaagtttatc
    24481 gcggttatgc cgcccacaaa gcagtcatcg actacagctt tcacggtgtc attcaacata
    24541 tcaatcacgc tatcctcgac gaaattccga tgatggttga ggaaggactg agcagtttta
    24601 aactctattt aacctatcaa tataaactca acgatgatga agtgttgcag gcattacgcc
    24661 gcttgcatga atccggcgcg ctgaccaccg tgcacccgga aaatgacgca gctatcgcca
    24721 gcaagcgggc ggagtttatc gccgcagggt taaccgcgcc gcgttatcat gccttgagtc
    24781 gacctctgga atgcgaagcg gaagccatcg cccgcatgat taacctggca caaattgccg
    24841 gtaacgcccc gctctatatc gtgcacctgt ctaacggctt aggtctggat tatctgcgtc
    24901 ttgcccgtgc gaatcaccag ccagtctggg ttgaaacctg cccacaatat ctcctgttgg
    24961 acgaacgcag ttacgataca gaagatggca tgaagttcat tcttagccca ccgctgcgta
    25021 acgtacgcga gcaggacaaa ctgtggtgtg gcatcagcga tggtgcgatt gacgtggtag
    25081 cgaccgatca ctgcacattc tcgatggctc aacgcctgca aatttctaaa ggcgatttca
    25141 gtcgctgccc aaatggctta cccggtgtgg aaaaccgcat gcagttactg ttttccagtg
    25201 gcgttatgac gggacgtata acaccggaac gttttgttga attaaccagc gcgatgcccg
    25261 ccaggctgtt tggcctgtgg ccgcaaaaag gattattagc gcccggttcc gatggcgacg
    25321 tggtgattat cgacccacgt cagagtcaac aaattcagca tcgccatctc cacgacaacg
    25381 ccgactactc gccatgggag ggttttacct gtcagggcgc gattgtcaga actttatccc
    25441 gtggtgaaac gattttctgt gacggcacct ttacaggcaa agccgggcga ggtcgtttcc
    25501 tgcgacgcaa accgtttgtc cctcccgtgc tctaaccact gccagggatc tatacacaat
    25561 caagaatgat gagtgaggaa aaatgagtaa gaaaattgtt ctcgccctgg gcgggaatgc
    25621 gctgggcgat gacctggccg gacaaatgaa agcggtaaaa attacttctc aggcaattgt
    25681 tgatttaatt gcccagggac atgaagttat cgtcactcac ggtaacgggc cacaggtggg
    25741 catgattaac caggcctttg aagctgccgc aaaaactgaa gcgcactcgc caatgctgcc
    25801 gatgtctgtc tgtgttgcct taagccaggg ttatattggt tacgatctac aaaacgcatt
    25861 aagagaagaa ctgctttctc gtggcattaa taaacctgta gcaacgctgg ttacccaggt
    25921 cgaagtcgat gctaacgacc cggcattcct caacccgacc aagccgatcg gttcgttctt
    25981 taccgagcag gaagcagagc aactgacaaa acagggttac actctgaaag aagatgctgg
    26041 tcgcggctat cgccgtgttg ttgcctcgcc gaaaccggtt gatattattg aaaaagagac
    26101 ggtaaaagcg ctggtagaag caggccaggt agtgatcacc gttggcggtg gtggcattcc
    26161 cgttattcgt gaagggaacc atctgcgcgg tgccagtgcg gttatcgata aagactgggc
    26221 cagcgcccgt ttagcagaaa tgattgatgc cgatatgctg atcattctga ctgcggtaga
    26281 aaaagtggcc attaacttcg gtaaagagaa tgaacagtgg ctcgatcgcc tgtcattaag
    26341 tgatgctgaa cgctttatcg aagaaggtca ttttgcgaaa ggttctatgc tgcctaaagt
    26401 ggaagctgcc gcctcgtttg ctcgctctcg cgcaggtcgt gaagcactga ttaccgtatt
    26461 gagcaaagcg aaagaaggga ttgaaggcaa aaccggaacg gtgatttgtc agtaatttaa
    26521 aggccgggtg ggtaatacca tccggccatt ttcatttaag ccacttccag cacttctttc
    26581 gtcgctttaa cgccctgatg catcaacatc attaacgcct caagtacgcc gccgccaatc
    26641 gcccgggctt tatcagaaac gctggtgaaa tcagccgttt caccacgagg atcgatatca
    26701 ccaattttaa aaccaccgac cactgccagt ccgtcgttca acaagccacg caccatcccc
    26761 gtcaacggtg ctttaatttc atgctcacca atccaggcaa tcacatcgcc ctctttcacc
    26821 agatcgccta atttcacgtt ggatcgcata atgcctgcag caggagcacg gatcacccgt
    26881 cgggtggtat gccccataat attgccagga acaccggtat tctcctgcgc acaaccagag
    26941 taaatcacct gaccgagcca gtgcccgcga tttgtttcaa ttaccgcatg acaatccttc
    27001 cctgcagtaa agcccggccc aagcgcgatt gttactggtg ccatatctgc ccgcgtcccc
    27061 aaattctgtt tcgccagaat agcgtccacc acgcaaagcg gtttcagttc atcaagcaat
    27121 gaacaggcgg gatctactat cacagggatg aatccgcatt cggtaagttt catcgcctcc
    27181 gcagagctgg ttgccaggcg ggcggtgacg ccttcgaccg tcatttcgcc atcgaacacg
    27241 gcctgggcaa atgccacggt acaacgaatc actgtcggtt tttccacttc cagcatgatc
    27301 actttaaaac ccgcatgata cagacgtagc gctacaccac tggcgatatc ccccgcacca
    27361 cgaatcacca ccagctggtg tccggaagga tgagaaggct tcatcatcag cccacccggc
    27421 gcgttatttt tcacctgtaa tatttctgcc agcacgctga tggcgatctc ctgcggcgtt
    27481 tccgcaccta tgttgtaacc aacgggcgca tgtaaacggg caatatgttc ttcagccacg
    27541 cctttctcac gcaattggcg caggaaaagc tgaaccttgc ggcgacttgc cagcaacccc
    27601 agccatgcaa tgggttgttc aatgagtttg tcgagggctt cacgatcctg attattcgtg
    27661 gcaatcagga caaaattatc agggcgaata tccagtgctt ccactgccgc accaaacgac
    27721 tcagcatgga gaagcgtggt tgatggtggg aatagttcag gattgaggct ttcgcgataa
    27781 atatcggcaa cggcgatatc aaatcctaat agcgccgcac tctgggctat cgcccggttg
    27841 acatgccccg cgccaatcaa caccagacgt ggacgcatac catggacgct gataaatact
    27901 gacattgcac ctccacaatc tgacccgaca gcatccgcac cgttacgagc catacgtcca
    27961 tggaataatc gcggcttacg ttcctgcaat gcctgaagcg actcttcaat caccttccgc
    28021 tcaaccattc cgccaccaat tgtaccgacg atagagccat cggcgcgcac taacatttgt
    28081 gcagaatggc ggggggttga gcctcggcta tcaacaattt gcgccatcgc aaacggacaa
    28141 ttttgctctt cgagttttgc agcctctgtg aaaatattca taccaacctc aatggatatc
    28201 ctttcagtaa cccggaatac ccgggccgct aattcacaaa tctgcgcgcg attgcaggat
    28261 gttcttgtat atcgcccagc cagattgcct ctacgtcgtg ttgttgcagc ggttgaagga
    28321 gctcgctttg cgcaatcgca ttctcacatt gagaaaaacg gttaatgaac cagactcgcc
    28381 gacaaccttg cggtacgttt ttaaacgccc cctgagggtg gcgaaccagc gcaacgagat
    28441 cgctcagttg caaggttgca tcaggtgtta acccggtaat atcagcaaac tgcgaccagc
    28501 gatggacatt ttctgtgctc actttcgcac ccaaaatatg tcctcccatc acagcaatca
    28561 cgcagcaact gcttttaggt atgcaaggtt cgtgctcatc aggcgctttt aacggcattc
    28621 cacgcgagcc atctgcctca atgagaatta cgtcacactc tgatcgttgt accagtgcat
    28681 caatcgcttc tggcgtaaat ccctgcactt ttccctggtt cgctttccag ctgtgaaaac
    28741 aaaatgaaat gggggatata aaagacgcat gaggaagcat ggcgggatca cgacagaaaa
    28801 ccacgggcca gtgagatgtt ggcataaaca tatgtgtcgt cgtagtgatt aatacacgcc
    28861 tgccgctcgc ctggaacagt tctgccagcc aaaaaagcaa gctggttttc cccccagcac
    28921 caacaacaga aatcacagtg ggacgtttct gcgcgcctaa atcaatgaat aatgccgatg
    28981 ggtcaattat acttttcacg cgtctttatg gacctctctg ggataaatta ccctcaactt
    29041 aatccatcag gaagtaacgc aattatcagg cgttattagc tcctgtaaat aatggaatca
    29101 ctatgtcagc catcgactgt ataattaccg ccgcaggatt atcatcaaga atggggcaat
    29161 ggaaaattat gttaccctgg caacagggaa caattcttga tacaagtatc aaaaatgcgt
    29221 tgcagttttg tagccgaatt attttagtca ccggctatcg tggtaatgaa ctgcacgaac
    29281 gttatgcgaa ccagagcaat atcactatta tccacaaccc agattatgcg cagggtttac
    29341 tgacatcagt aaaggccgca gtacccgcgg tgcaaacaga acattgtttt ctcacccacg
    29401 gagatatgcc aaccctcacc atcgatattt ttagaaaaat ctggtcgtta cgaaatgatg
    29461 gcgcaatact gccactccat aatggcatcc ccggccatcc gattttagtg tcaaaaccat
    29521 gcctgatgca ggcaatccag cgacccaatg tcaccaatat gcgtcaggca ctccttatgg
    29581 gagaacatta ttccgtcgaa atagaaaatg cagaaataat tttagatatt gatactcctg
    29641 acgactttat tacagcgaag aaaaggtata ctgaaattta gaaaacgtag ttaaacgatt
    29701 gcgttcaaat atttaatccc tccagcactc tttatcataa tgataaggaa tcactgattt
    29761 tgagaaaaga gaaaaagtga taaatgggct ggcaataata attttcagga gtgattttaa
    29821 ttctcatttg gcacctgtga gttcattcac aaaaagccat taagtttcga gccgcctcac
    29881 atttttttat atttcccgcc aaacctggca agagtggtgc gattgttgct ctatccccct
    29941 aaaccaccgg atttctcaac accggtcact caatgatatc tgtataagct aaggagaggg
    30001 ttatggggga tattatgcgt cccattccgt ttgaggaact tttgacgcgc atatttgatg
    30061 aataccaaca acaacgctca atctttggta ttcccgagca acagttttac tcacccgtaa
    30121 aaggtaaaac tgttagcgtc ttcggtgaaa cctgtgccac tcccgtcggc cctgccgctg
    30181 gcccgcacac gcagctcgcg caaaatattg tcacttcctg gctgactggc ggacgcttca
    30241 tcgaactaaa aaccgtccaa attcttgacc gcctggagct ggaaaagccc tgtatcgatg
    30301 ccgaagacga gtgctttaac accgaatggt ctaccgaatt taccctgctt aaagcctggg
    30361 atgaatacct caaagcctgg tttgccctgc accttctcga agcgatgttc cagccttctg
    30421 attcctgtaa atcgttcatc tttaatatga gcgtcggtta caacctcgaa ggtattaagc
    30481 aaccgccgat gcagcagttc atcgacaata tgatggacgc atctgaccat ccgaaattcg
    30541 ctcaataccg cgatgcactt aataagctac tccaggatga cgcattttta gcccgccacg
    30601 gcttacagga aaaacgcgaa agcttgcaag ccttacccgc tcgcatcccc accagcatgg
    30661 tacaaggggt taccctctcc accatgcacg gctgtcctcc gcatgaaatc gaagccattt
    30721 gccgctacat gctggaagaa aaagggctca acacctttgt gaaactcaac ccgaccttac
    30781 tggggtacgc gcgtgttcgt gagatcctcg atgtctgcgg tttcggttac atcggcttaa
    30841 aagaagagtc atttgatcac gacctcaagc tgacgcaagc actggaaatg ctggaacgcc
    30901 tgatggcact ggcaaaagaa aaatcactcg gctttggcgt aaaactgact aacactctcg
    30961 gcaccattaa taacaaaggc gcactgcccg gtgaagagat gtatatgtca ggccgtgcgc
    31021 tgttcccgct ctccatcaac gttgcagcag ttctctctcg cgcctttgac ggcaaactgc
    31081 ccatttctta ttccggtggt gccagtcagc tgactatccg cgatattttt gatactggta
    31141 ttcgccctat tactatggca accgacctgc tgaaacctgg cggctatctg cgcttaagtg
    31201 cctgcatgcg cgagctggaa ggctccgacg cctggggact tgaccacgtt gacgtcgaac
    31261 gactgaacag actggcagca gacgcgttaa ccatggaata cacccagaaa cactggaagc
    31321 cagaagagcg tattgaagtg gctgaagacc tgccgctgac cgattgctac gttgccccct
    31381 gtgttactgc ctgcgctatc aagcaagata ttccggaata catccgtctg cttggcgaac
    31441 accgctatgc cgacgcgctg gaactcatct atcaacgcaa cgccctgccc gccattaccg
    31501 gtcatatttg cgatcaccag tgccaataca actgtacccg cctggattac gacagtgcgc
    31561 tgaatatccg cgaactgaaa aaagtcgcgc tggaaaaagg ttgggatgaa tataagcaac
    31621 gctggcacaa accagccggt tctggttcac gccatccggt tgccgtgatt ggtgcaggtc
    31681 cggcgggtct ggccgcaggt tacttccttg ccagagcggg ccatccggtt acgctgtttg
    31741 aacgcgaagc caacgcgggc ggcgtggtga aaaatatcat tcctcagttc cgtattcctc
    31801 cagagttaat tcagcacgat atcgattttg ttgccgctca cggcgtgaaa tttgagtatg
    31861 gctgctcacc cgatctgacc gttgaacagt taaaaaatca gggcttccac tatgttctga
    31921 ttgccaccgg cactgataaa aatagcggtg tgaaactggc gggcgacaac caaaatgtct
    31981 ggaaatcact ccccttcctg cgtgaataca acaagggcac agagctcaag ctgggcaaac
    32041 atgtggtcgt tgtcggggcg ggtaacaccg caatggactg cgctcgtgcg gcgttacgcg
    32101 ttccaggcgt agaaaaagca acggtcgttt accgtcgttc actgcaagag atgccagcat
    32161 ggcgcgaaga gtatgaagaa gcgttgcacg acggcgtgga gttccgtttt ctgaataatc
    32221 cggaacgttt cgatgctgat ggcaccttaa ccttgcgcgt tatgtcgctt ggcgaacctg
    32281 atgagaaagg tcgtcgccgt ccggttgaaa ccaacgaaac agtaacactg catgtagaca
    32341 gcctgatcac cgccattggt gaacagcagg atactgaagc cctgaatgcg atgggcgtgc
    32401 cgctggacaa aaacggctgg ccagacgtcg accataatgg cgaaactcgt ctgactgacg
    32461 tctttatgat cggcgacgta cagcgcggac catcctccat tgtcgctgct gtcggaaccg
    32521 cgcgtcgggc gaccgatgcc atccttagtc gggaaaatat ccgttcccac cagaacgata
    32581 aatactggaa caacgttaat ccggcggaaa tctatcaacg caaaggcgat atctctgtca
    32641 ccctggtgga tagcgacgat cgtgacgcgt ttgtcgccca ggaagccgca cgctgccttg
    32701 aatgtaacta cgtttgcagc aagtgtgtgg atgtctgccc gaaccgcgcc aacgtatcca
    32761 ttgcggtccc aggcttccag aaccgtttcc agacgctgca cctcgacgct tactgtaacg
    32821 aatgtggcaa ctgcgcccaa ttctgcccgt ggaacggtaa accgtacaaa gacaaaatca
    32881 ccgtcttcag cctgtcgcaa gactttgata acagcagcaa cccaggcttc cttgtggaag
    32941 attgccgggt acgcgtacgt ctgaataacc aaagctgggt gttaaacatc gacaacgaag
    33001 gtcagttcaa caacgtacca ccggagctga acgatatgtg ccgcatcatt agccatgtcc
    33061 accagcatca tcattatctg ctgggccgtg tggaggtgta atcatgttga ttctgaagaa
    33121 tgtcaccgca gtgcagttac acccagcgaa agtgcaggaa ggcgttgata tcgccatcga
    33181 aaatgatgtg attgtcgcta tcggcgatgc cctgacgcaa cgctaccccg atgccagcta
    33241 caaagagatg catggtcgga ttgtgatgcc aggaattgtc tgctcgcaca accattttta
    33301 ctcggggctg tcccgcggaa ttatggcaaa catcgccccc tgcccggatt tcatctcaac
    33361 gctgaaaaat ctctggtggc gactcgatcg cgcccttgat gaagagtcgc tctattacag
    33421 cggactgatt tgttccctgg aagcgattaa gggcggatgt acatcggtta tcgatcacca
    33481 tgcctctccg gcgtatatcg acgggtcgct ctccacattg cgcaacgcat ttttaaaagt
    33541 tggcctgcgc gcgatgacct gttttgaaac tactgaccgt aacaacggta tcaaagagtt
    33601 gcaggaaggt gtagaagaaa acatccgctt cgcccgtcag attgatgatg cgaagaaagc
    33661 ggcaactgaa ccgtatctgg tagaggctca tattggtgct cacgcgccgt ttactgtgcc
    33721 ggatgccggt ctggaaatgc tgcgtgaggc cgtgaaagcc acaggccgtg gtttacatat
    33781 tcacgctgcg gaagaccttt acgatgtttc ccatagtcat cactggtacg gcaaagatct
    33841 gctggcacga ttagcgcaat tcgatctcat cgacagcaaa acgctggtcg ctcatgggct
    33901 ttatctgtct aaagacgaca tcgcgttgct caatcagcgt gatgcattcc tggtgcataa
    33961 cgcccgttca aacatgaaca accatgtcgg ctacaaccat catctttgcg acatccgcaa
    34021 tctggcgttg ggtacggacg gtattggttc agatatgttt gaagagatga aatttgcctt
    34081 ctttaaacat cgcgatgcgg gtggtccgct gtggcctgac agctttgcca aagctctggc
    34141 taacggcaac gaactgatga gccgcaactt tggcgcgaaa tttggccttc tggaagccgg
    34201 ttacaaagcc gatttaacca tttgcgatta caactcgccg acgccgctgc tggcagacaa
    34261 tatcgccggg catatcgctt tcggtatggg ctcaggcagc gttcacagcg tgatggtcaa
    34321 tggtgtgatg gtctatgaag accgtcagtt taacttcgat tgcgattcca tttatgcgca
    34381 agccagaaaa gccgctgcca gtatgtggcg tcggatggat gcgctggcat aaataacagc
    34441 tcccctcttc tttcagagaa gaggggaaat taaggaaaga cgatgattga acaatttttc
    34501 agacccgact ctgtcgaaca ggcgctggaa ctgaagcgcc gctaccagga tgaagccgtc
    34561 tggttcgccg ggggcagcaa actcaacgct acaccaaccc gtaccgataa aaagattgcc
    34621 atttccttac aggatctgga actggactgg gttgactggg ataacggtgc actgcggatt
    34681 ggcgcgatgt ctcgcttgca aacactgcgt gatgcgcgat ttattcctgc agcactgcgt
    34741 gaagctctcg gttttgttta ctcacgccat gttcgtaatc agtcgaccat tggtggtgaa
    34801 attgccgccc ggcaagaaga gtcagtgctg cttcccgtcc tgctggcact ggatgctgaa
    34861 ctggtttttg gcaacggcga aactctgtca attgaggagt acctggcctg cccatgcgat
    34921 cgcctgctaa ccgaaatcat cattaaagat ccgtatcgca cctgtgcgac tcgcaaaatt
    34981 agccgttctc aggcaggttt aaccgtcgtg acggcagccg ttgcaataac agaccacgac
    35041 ggtatgcgaa ttgcgctgga tggcgtagcc agtaaagcac tgcgtctgca cgatgttgaa
    35101 aaacaaaacc tggaaggtga tgcccttgaa caggctgtcg ccaacgccat tttcccgcag
    35161 gaagatttgc ggggcagcgt ggcctataaa cgctatatca cgggagttct ggtagccgac
    35221 ctttatgccg actgccaaca ggctggggag gaagccgtat gatcatccac tttactttaa
    35281 atggcacgcc ccaggagcta accgttaatc ctggtgaaaa cgtgcaaaag ctgttgttta
    35341 acatgggaat gcactctgta cgcaacagcg atgatggctt tgggtttgcc ggttctgacg
    35401 caattatctt taacggtaat atcgttaacg cgtccttgct tatcgccgca cagttagaga
    35461 aggcagatat tcgtaccgca gaatctctgg gcaaatggaa tgagttaagt ctggttcaac
    35521 aggccatggt tgatgttggc gtggtgcagt ctggttataa cgatccagct gcggctctga
    35581 ttatcaccga tcttctcgat cgcatcgacg cacctacccg cgaagagatc gacgacgcgc
    35641 tctcaggtct gtttagccgc gatgctggct ggcagcaata ctatcaggtc attgaactgg
    35701 cggttgcacg taaaaataat ccgcaggcca ccattgatat cgctccgact ttccgtgacg
    35761 accttgaagt cattggcaag cattatccta aaactgatgc cgcgaaaatg gtgcaggcga
    35821 aaccctgcta tgttgaagat cgcgtaacgg ctgacgcctg cgtcattaaa atgttacgta
    35881 gcccacacgc tcacgcactg attactcatc tggatgtcag caaagctgaa gccttaccgg
    35941 gcgtcgttca cgttattact cacctgaatt gcccggatat ttactatacc ccgggtggtc
    36001 agagcgcacc ggaaccgtca ccgcttgacc gccgaatgtt cggcaagaaa atgcgtcacg
    36061 tcggcgatcg cgttgctgcg gtcgtcgctg aaagtgaaga cattgcgctc gaagcactga
    36121 agctcatcga cgttgaatat gaagtgctta agccggtaat gtcgattgac gaggcaatgg
    36181 cggaagatgc gcctgtcgtg cacgatgaac cggtggtgta tgttgctggt gcaccagata
    36241 ctctggagga agataactgt catgcagccc agcgcggcga gcatatgatc atcaacttcc
    36301 cgatcggttc tcgcccacgc aaaaatatcg ctgccagtat tcatggtcat attggtgata
    36361 tggacaaagg ctttgccgat gccgatgtga tcattgagcg aacctataac tcaacgcagg
    36421 cacagcagtg cccgactgaa acacatatct gctttacccg tatggacggc gatcgtctgg
    36481 tgatccacgc ctccacccag gtaccatggc acttacgccg ccaggtcgcg cgcctcgtgg
    36541 gcatgaaaca acataaagtt catgtcatta aagagcgagt tggcggcggt tttgggtcta
    36601 aacaggacat cctgctggaa gaagtgtgcg cctgggcaac ctgcgtgacc gggcgtccgg
    36661 tactgttccg ctacacccgt gaagaagagt ttattgctaa cacctctcgt cacgttgcga
    36721 aagtcaccgt caaactggga gcgaaaagag atggtcgcct gacagcagtg aagatggatt
    36781 tccgcgccaa cactggccct tacggcaacc actcactcac cgtaccatgt aacggaccgg
    36841 cgctgtcgct gccgttatat ccgtgcgata acgtcgattt ccaggtcacc acctactaca
    36901 gcaacatttg cccaaatggt gcttatcagg gttatggcgc accgaaaggt aacttcgcta
    36961 tcaccatggc attagcggaa ctggctgaac agttacagat cgaccaactg gaaattatcg
    37021 aacgtaaccg ggtacacgaa gggcaagagc tgaaaattct cggtgcaatc ggtgaaggta
    37081 aagcgccgac ctccgttcct tccgccgcca gctgcgcact ggaagagatc ctgcgtcagg
    37141 gtcgcgagat gatccaatgg tcttcaccaa aaccacaaaa cggtgactgg cacatcggtc
    37201 gtggcgtcgc cattatcatg cagaaatcag ggatcccaga tatcgatcag gctaactgca
    37261 tgatcaaact ggaatcagac ggtaccttta tcgttcattc tggcggtgcg gatattggta
    37321 ctggtctgga tactgtggtg acgaaactgg cagcagaagt gctgcactgc ccaccgcagg
    37381 acgtgcatgt tatctccggt gataccgatc atgcgttgtt tgataaaggc gcatatgcct
    37441 cgtccggtac ttgcttctcg ggtaacgcgg cgcgtttggc agcagaaaat ctgcgggaga
    37501 aaattctgtt ccacggtgcg caaatgttgg gtgagccagt ggcagatgtc caactagcaa
    37561 cgccgggcgt cgtacgcggc aagaaaggcg aagttagttt cggagagata gcccataaag
    37621 gcgaaaccgg caccggcttt ggttcactgg tgggaactgg cagttatatc acgcctgatt
    37681 tcgccttccc gtatggcgca aacttcgctg aagttgccgt caacacgcgt acgggtgaaa
    37741 tccgcctgga taaattctac gccttgctgg actgcggtac accggtcaat ccagagttag
    37801 cgttgggaca aatctacggt gccactctgc gagctatcgg ccacagtatg agcgaagaga
    37861 tcatttatga cgccgaaggt cacccgttaa cgcgtgattt acgcagttac ggcgcaccga
    37921 aaattggtga cattccgcgt gatttccgcg ctgtgctggt gccgagcgac gataaagtcg
    37981 gcccattcgg ggcgaaatcg atctcggaaa tcggtgtaaa tggcgccgct ccggcgattg
    38041 ctaccgcaat tcacgatgcc tgcggcatct ggttacgcga atggcatttc acaccggaga
    38101 aaatactcac cgcgctggaa aaaatataag taataaatgg ccgcttcggc ggcctgtttc
    38161 tctgtttgaa cgatgactaa atagtgactg cgaaaatgag ataagccgtt ctcaaggcgt
    38221 cctgcgcccc aaattcgtgg gaatttattt tactaattca gatgatcaaa tttactttaa
    38281 aaggagtgaa gggatgtctg atataaacca tgcaggttct gaccttatat ttgaactgga
    38341 ggatcgccct ccctttcatc aggctctcgt aggtgccatt acccatctgt tggcaatttt
    38401 cgttccgatg gtaacccccg cgttaatcgt gggtgcgacc ttacagcttt ccgctgaaac
    38461 aactgcctat cttgtttcta tggcgatgat cgcctctggt attggtacct ggttacaagt
    38521 aaaccgctac ggcatcgtcg gttctggtct tctctcaatt cagtcagtca atttttcatt
    38581 tgttacggtc atgattgcgc tgggcagcag catgaaaagc gacggttttc acgaagagtt
    38641 aatcatgtcg tcgctcctcg gcgtctcctt cgttggcgca tttctggttg tcggctcttc
    38701 ttttatcctg ccctatttac gtcgggttat tacgcctacc gtcagcggta ttgtggtact
    38761 gatgatcggc ttaagcctga ttaaagtcgg cattatcgat tttggtggag gatttgcagc
    38821 caaaagcagc ggtacgttcg gcaattacga acatctcggc gttggtttat tggttttaat
    38881 tgtggtgatc ggctttaact gctgtcgcag tccgttgcta cgcatgggag ggatcgccat
    38941 tgggctatgt gtcggctata tcgcatcgtt atgcctgggc atggtggatt tcagcagtat
    39001 gcgcaatttg ccgttaatca ccatcccgca tccgttcaaa tacggcttta gttttagctt
    39061 ccatcagttc ctggtggttg gcacgattta tctgcttagc gtgctggaag cagtcggcga
    39121 tatcaccgcc acggcaatgg tttcccgccg ccccattcag ggggaagagt atcagtcccg
    39181 actgaaaggc ggcgtgctgg cagatggtct ggtttctgtt atcgcctccg ctgtcggttc
    39241 attaccatta accacgtttg cgcaaaataa tggggttatt cagatgactg gcgtcgcttc
    39301 acgttatgtc gggcgaacca tcgcggtaat gctggttatc ctcggcttat ttccgatgat
    39361 tggcggcttc ttcacgacca ttccctcggc agttctggga ggcgcaatga cgttgatgtt
    39421 ttccatgatt gccatcgctg ggattcgcat catcatcacc aacggattaa agcgccgaga
    39481 aacacttatt gtcgccacgt ctttaggttt agggcttggc gtctcctacg atcccgaaat
    39541 ttttaaaata ttgccagcct ctatttatgt attagttgaa aaccctattt gtgctggcgg
    39601 gttaactgcg attttattaa atattatcct ccctggtggc taccgacagg aaaacgttct
    39661 gcctggtatt acctcagcgg aagagatgga ttaacagtaa aggagtcaat gatgtcagga
    39721 gaacacacgt taaaagcggt acgaggcagt tttattgatg tcacccgtac ggtcgataac
    39781 ccggaagaaa ttgcctctgc gctgcggttt attgaggatg gtttattact cattaaacag
    39841 ggaaaagtgg aatggtttgg cgaatgggaa gacggaaagc atcaaattcc tgacaccatt
    39901 cgcgtgcgcg actatcgcgg caaactgata gtaccgggct ttgtcgatac acatatccat
    39961 tatccgcaaa gtgaaatggt gggggcctat ggtgaacaat tgctggagtg gttgaataaa
    40021 cacaccttcc ctactgaacg tcgttatgag gatttagagt acgcccgcga gatgtcggcg
    40081 ttcttcatca agcagctttt acgtaacgga accaccacgg cgctggtgtt tggcactgtt
    40141 catccacaat ccgttgatgc gctgtttgaa gccgccagtc atatcaatat gcgtatgatt
    40201 gccggtaagg tgatgatgga ccgaaacgca ccggattatc tgctcgacac tgccgaaagc
    40261 agctatcacc aaagcaaaga actgattgaa cgctggcaca aaaatggtcg tctgttgtat
    40321 gcgattacgc cacgcttcgc ccctacctca tctcctgaac agatggcgat ggcgcaacgc
    40381 ctgaaagaag aatatccgga tacgtgggta catacccatc tctgtgaaaa caaagatgaa
    40441 attgcctggg tcaaatcgct ttatcctgac catgatggtt atctggatgt ttaccatcag
    40501 tacggtctga ccggtaaaaa ctgtgtcttt gctcactgcg tccatctcga agaaaaagag
    40561 tgggatcgtc tcagcgaaac caaatccagc attgctttct gtccgacctc caacctttac
    40621 ctcggcagcg gcttattcaa cttgaaaaaa gcatggcaga agaaagtcaa agtgggcatg
    40681 ggaacggata tcggtgccgg aacaactttt aacatgctgc aaacgctgaa cgaagcctac
    40741 aaggtattgc aattacaagg ctatcgcctc tcggcatatg aagcgtttta cctggccacg
    40801 ctcggcggag cgaaatctct gggccttgac gatttgattg gcaacttttt acctggcaaa
    40861 gaggctgatt tcgtggtgat ggaacccact gccactccgc tacagcagct gcgctatgac
    40921 aactctgttt ctttagtcga caaattgttc gtgatgatga cgttgggcga tgaccgttcg
    40981 atctaccgca cctacgttga tggtcgtctg gtgtacgaac gcaactaata ataaaacttt
    41041 aacatcctcg tgaggacatc attatgtctg gagacatcct acaaacaccg gacgcaccaa
    41101 agccacaggg cgcgctggat aattatttta aaattaccgc tcgtggcagt accgttcgtc
    41161 aggaagtact ggctggctta acgacctttc tggccatggt ttattccgtt atcgtcgttc
    41221 cgggaatgct gggcaaagca ggttttcctc ccgcagctgt gtttgttgcc acctgtctgg
    41281 tcgcgggctt cggctcgttg ctgatggggt tgtgggccaa tttgccaatg gcgattggtt
    41341 gtgcgatttc cttgacggcg tttaccgcat tcagtctggt actcgggcaa caaattagcg
    41401 ttcctgtcgc actgggcgcg gtatttctga tgggcgtcat cttcaccgcc atttccgtaa
    41461 ccggtgtgcg tacctggatc ttacgtaatt tgccgatggg gattgctcac ggtacaggta
    41521 tcggtatcgg cctgtttctg ctgctgattg ctgctaacgg tgtgggaatg gttatcaaaa
    41581 acccgattga aggcttgcca gtggcgctcg gtgcgtttac ctccttcccg gtgatgatga
    41641 gcttgctggg gctggcggtt atcttcggtc tggagaagtg tcgcgtaccc ggcgggatct
    41701 tgttggtgat tattgcaatt tcgatcatcg gcttaatctt tgacccagcg gtgaagtacc
    41761 acggtctggt ggcgatgcca agcctgactg gcgaagatgg taagtctctg attttcagcc
    41821 tcgatattat gggcgcactc cagccaactg tacttccgag tgtactggca ttggtgatga
    41881 ccgcagtgtt cgacgccact ggcaccatcc gtgccgtcgc cggtcaggcg aatttgttgg
    41941 ataaagacaa ccagatcatc aacggcggca aagccctgac cagtgactca gtaagttcaa
    42001 tattctccgg cctggtgggc gcagcgcccg cggcggttta tatcgaatca gcggcaggaa
    42061 ccgccgccgg ggggaaaacc ggtttaaccg caaccgtagt gggggcgtta ttcctgctga
    42121 ttctgttctt atcaccgctg tcatttttga tccctggtta cgccactgcc cccgctctga
    42181 tgtacgtagg tttgctgatg ttaagtaacg tctcgaagct ggatttcaat gattttattg
    42241 acgcaatggc tggcctggtg tgtgccgtgt tcatcgttct gacttgtaat atcgttaccg
    42301 gtattatgct tggctttgtg acactggtcg taggccgcgt ctttgcacgc gaatggcaaa
    42361 agctgaatat tggtacggtg atcattactg ccgcactggt cgcattttac gcgggtggtt
    42421 gggcaatcta atagatcctc cgcgcctttc agcgcggaga tttctttcac agaggattct
    42481 caccgctggc aacaatacgc tgctggcgga ctcgcctgag ttctctttca gtaagcaact
    42541 gcagcgcctg tgttgggcaa gattcaacgc aggctggccc ctcttcccgc tgttcacaga
    42601 gatcgcattt cacgatttgt tgccgggtat cccccggcaa cgactgaatg gtgatcattc
    42661 caaacgggca cgcactgacg caactctgac agccaataca acgggcagaa ttggtctgca
    42721 ccacctgctc gcccatcgtc agcgccccca cggggcaagc gctaacacaa ggggcgtttt
    42781 cacactgatg gcacatcacc ggcgcactga tgctgtccag tcgctgtacc ttcagccggg
    42841 gtaaaaagac atcggcattc aactcctgtt ctgaagggtg agcgaccaca caggctactt
    42901 cacaagtacg gcagccaata cagtcagccg gattaacgat aattaacgat ttcatcatga
    42961 tgccttcgta tcaaacagag ttaacatatc gcgcgccgcc tgccttcctg cggccattgc
    43021 agtgacaacc agatccgcgc catgaacagc atcaccacca gcaaagactt ttttcagatg
    43081 cgtctgggta ggtaaatacc cgacgtcacc ggtttgaatc aggccccatt tatcgagttt
    43141 aattccgctg ccctgcaacc acggcatggc atgcgcctgg aaaccaaagg ccataatgag
    43201 aacatcggcg ggcaattcaa actctgaacc cgctaccgga cgaggacgac ggcgaccatc
    43261 cggccccggc tcacccatgg cggtacgaat caggcccacc gcagttaaac gcccatcttc
    43321 gtcacaagcg atatattgcg gctgaacatt gaactgaaac tcgacacctt cctcgcgcgc
    43381 attgaccacc tctttgcgcg agcccggcat actgacttca tcacgacgat acgcgcaggt
    43441 cacgctggcg gcattgaggc ggatggaagt ccgcaaacaa tccatggtcg tatcgccacc
    43501 gcccaatacc acgactcgct taccttccac atccgtcagc ggatactctt cagactccgg
    43561 caaccccatg agctggcggg tatgggcagt caggaacggt aacgcctgaa tgacaccggg
    43621 cgcatcttca tgcggcagat ctgctcgcat catcccgtaa gtccccacgc cgataaaaac
    43681 tgcatcatat tcagaagtta aatcgctaaa ggtaatatcg cggccaattt cacagttaag
    43741 atgaaagtcg attcccattg cggtgaatat ctctcgccgc tggcttaata ccgttttatc
    43801 gagtttgaaa ggaggtatgc caaaagtcag cataccgcca atttctggat ggcgatcaaa
    43861 gacatcaacc tgaactcctg cgcgcgccaa aatatcagca caccctaacc ctgcaggccc
    43921 tgcgccaatc accgccactt tttcgatacg gggaacaact ttgctgacat cgggacgcca
    43981 gcccatcgcc agcgcggtat cggtaatgta gcgttccaga ttcccgatag agactgcgcc
    44041 agagtgatct ttcaaagtac atgcgccttc acaaagacgg tcctgtggac ataccctgcc
    44101 gcagatttct ggcaaggaac tggtctggtg gcaaagttct gccgcttcaa taatctttcc
    44161 ttcctgcacc agacggatgt aatccggaat agcgttatgc agcgggcaat gccagttgca
    44221 gttagctttt tcggcgcaat aaacacagcg gtcactctca taagtcgctt gttgtggatc
    44281 cagcccgtaa tagatttcgc caaagtgggt tttccgttca ctcgctgaaa ttttatctgc
    44341 gcctttacgc gagttaacgg ggagcaacgc tgcactgcga gatggctgag cgtctgatga
    44401 cgcttttcct gctgccgttt tgcgctggcg ggccaccttt atctgctgca accctttatc
    44461 gtccatcagc cgtaacgcct gtgttgggca gacttcaata caggcttgcg tgccggaact
    44521 gcgctggtta caaaggtcgc atttctgcgc aatcgtatcg accatctcaa cgacgccaaa
    44581 ggggcaagcg attgcgcatc ttttacaacc aatacatttt tgctcgtcca gttgtacgct
    44641 atcgggctgg aaagtcagag cattaaccgg acaagccgta acgcaagggg cattgttgca
    44701 gtgatggcag gccaccggat tcgcagcctg gcctttccct acaacgtgga tacgcggtcg
    44761 aaagtcactg tgactcagcg gccagttttc ttgattatgt gccatcgcac aggcaatttc
    44821 acaagcatga cagcctatac attccgcagc ttcagcagcg ataaacttat tcatttgcat
    44881 ccctttcatt taatgagtta tgtcttctca aatttcgcga tgcaataaat gggcatgtat
    44941 taggtagcat ggccgattta attataattt tgcgactgag ttcaaattat tgcccctcaa
    45001 tctatgcagc tttcattatt tcaacattgc cgatcaaaag aatgctatcc agataaaaac
    45061 gattaaatgc gagagtgcga catgccagaa tgattaatga aatatcaata tgagaaaatt
    45121 acagataatt ccattgtcag gttctcttct cattttcgtg gtttaaccac gattatgtga
    45181 ttacctccgc aattcctgtc tctaactccc cttcctcgca aaactggcac tccacgagca
    45241 tgtatttaga cagtttcatg aacgtaaacg gttgcttttt actctggcgg gcgaaaggag
    45301 aaacactgat gagcgccata gattcccaac ttccctcatc ttctgggcaa gaccgcccaa
    45361 ctgatgaggt tgaccgcata ttatcaccag gaaagctgat catactcggt ctgcaacacg
    45421 tccttgtcat gtacgcaggt gcagtcgctg ttcctcttat gattggcgac cgactgggcc
    45481 tctcaaaaga agctattgcg atgctcatta gctcggatct cttttgctgc gggatcgtca
    45541 cattattgca atgtatcggt atcggccgct ttatggggat ccgcctgccg gtgattatgt
    45601 cggtgacctt cgccgctgta acaccaatga tagccattgg gatgaacccg gatatcggcc
    45661 tgctggggat atttggtgcc actatcgccg cgggttttat caccacatta ttagcgccac
    45721 ttatcggtcg cttgatgcct ttattcccgc cactggttac cggtgtggtt attacttcta
    45781 tcggacttag catcattcag gtgggtattg actgggccgc aggaggtaaa gggaatccgc
    45841 aatatggtaa tcccgtttat ttaggtatct cctttgccgt cttaattttt atcttgctca
    45901 ttactcgcta tgcgaaagga tttatgtcca acgtcgccgt attactgggg attgtatttg
    45961 gctttttact ttcgtggatg atgaatgaag tcaatttatc cgggctacat gatgcctcgt
    46021 ggtttgcgat tgtcaccccg atgtcgtttg gtatgccgat ttttgatccc gtttccattc
    46081 tgaccatgac tgccgtgtta atcatcgtgt ttatcgagtc gatggggatg ttcctggcac
    46141 tgggtgaaat agttggtcgt aaactctctt cacacgatat tattcgcggg ctgcgtgtcg
    46201 atggcgtagg gacaatgata ggcggaacgt ttaacagctt cccccatacg tcattttctc
    46261 aaaacgttgg cctggttagc gtgacgcgcg ttcatagccg ctgggtgtgt atttcttcgg
    46321 gaattatatt aatcctgttt ggcatggtgc caaaaatggc ggtgctggtc gcctccattc
    46381 cgcaatttgt gctgggcggc gctggtctgg tgatgttcgg catggtactg gcgacaggga
    46441 ttcgaattct gtcgcgctgt aactacacca ccaaccgtta caacctctat attgtggcga
    46501 tcagtctcgg cgttggcatg actccgacgc tctctcacga tttcttttct aagttaccgg
    46561 ccgtactgca accgttgctg catagcggca ttatgctcgc aacccttagc gccgttgtgc
    46621 tgaacgtctt ctttaatggc tatcagcatc atgctgatct ggtgaaggaa tccgtctctg
    46681 ataaagattt aaaagtcagg acagtacgta tgtggcttct gatgcgcaag ctgaagaaaa
    46741 atgagcatgg agaataatat gaatctttta atgcgcgcta tattcagtct gctgttgctt
    46801 tttactctct ctattcctgt catttctgac tgtgttgcaa tggccattga aagtcgcttc
    46861 aaatatatga tgctactttt ttaaatggtt tttacctgtc gacatccgct caaaacgggc
    46921 ggttgtcgat aaacgctcac ttggttaatc atttcactct tcaattatct ataatgatga
    46981 gtgattagaa ttacatgtga gaaattatgc aaacggaaca cgtcatttta ttgaatgcac
    47041 agggagttcc cacgggtacg ctggaaaagt atgccgcaca cacggcagac actctcttac
    47101 atctcgcgtt ctccagttgg ctgtttaatg ccaaagggca attattagtt acccgccgcg
    47161 cccttagcaa aaaagcgtgg cctggcgtgt ggactaactc ggtttgtggg cacccacaac
    47221 tgggagaaag caacgaagac gcggtgatcc gccgttgccg ttatgagctt ggcgtggaaa
    47281 ttacgcctcc tgaatctatc tatcctgact tctactatcg cgccaccgat ccgaatggca
    47341 ttgtggaaaa tgaagtgtgt ccggtatttg ccgcacgcac caccagtgcg ttacagatca
    47401 acgatgatga agtgatggat tatcaatggt gtgatttagc agatgtatta cacggtattg
    47461 atgccacgcc gtgggcgttc agtccgtgga tggtgatgca ggcagccaat agtgaagcaa
    47521 gaaaattgtt gtctgctttc gcgcagcaca attaaaaaac cccgacattt gccggggttg
    47581 tgagcataac gtaatgctta tttcaccgga cgcatcgccg ggaacagaat aacgtcgcgg
    47641 atggtatgac tgttggtgaa cagcattacc atacggtcga taccaattcc cagacctgct
    47701 gtcggcggta agccgtgttc cagtgcggtg acgtaatctt cgtcgtagaa catcgcttcg
    47761 tcgtcacctg catctttcgc ggcaacctga tccaggaagc gctgcgcctg atcttccgcg
    47821 tcattcagct cgctaaagcc gttaccgatt tcacgcccac caatgaagaa ctcaaagcgg
    47881 tctgtgattt ccgggttaac gtcgttacga cgcgccagag gagaaacttc tgccggatat
    47941 tcagtaatga aggtcggctg gatcagatgt gcttctgcca cttcttcgaa gatctcggta
    48001 acgatacggc ccagacccca gctcttctca acgtggatgc cgatagattc agcaatcgct
    48061 ttcgcagagt cgaagttgtc cagatccgcc atgtcggttt ccgggcgata tttcttgatc
    48121 gcttcacgca tggtcagttt ttcgaacggt ttaccgaagt ccagcgttac gtcgccgtag
    48181 gtcacttccg tcttaccgag aatatcctgt gccagagtac ggaacagcga ttcggtcagc
    48241 tcgatcagat ctttgtaatc tgcgtaagcc atatagagtt ccatcatggt gaactctggg
    48301 ttatgacgta cggaaatacc ttcgttacgg aagttacggt tgatttcgaa tacacgctcg
    48361 aagccaccga ccaccagacg cttgaggtac agttccggcg cgatacgcag gtacatgtcg
    48421 agatccagcg cattatggtg ggtgatgaac ggacgcgcag cggcaccgcc agggatcacc
    48481 tgcatcatcg gcgtttcaac ttccataaag ccgcggttca ccatgaactg gcgaatacca
    48541 gagaggatct gcgagcgcac tttaaaggtg ttgcgggatt catcgttaga gatgagatcc
    48601 agataacgct gacgatagcg cgcttcctga tcctgcaagc cgtggaattt atccggcagc
    48661 ggacgcagtg ctttggtcag cagacgcagc tcggtacagt ggatagacag ttcgccggtt
    48721 ttggttttga acagtttacc tttcgcgccg aggatatcgc cgaggtccca ttttttgaac
    48781 tgctcgttgt aaacgccttc cggcagatcg tcacgggcaa cgtacagctg aatgcggcca
    48841 cccacatcct gcagggtaac gaaagacgct ttacccataa tacgacgggt catcatgcgg
    48901 ccagcaacgg caacttcgat gttcagcgct tccagttctt cgttctcttt accgtcgaat
    48961 tctgcgtgca attggtcaga ggtatgatcg cgacggaaat cgttcgggaa ggcaatcccc
    49021 tgctcgcgca ggttcgccag cttctcacga cgcgttttca gttcattgtt aagatcgact
    49081 accgcgtcag cgccctgtgc gtgttgttca gacatgttgg ttcctcataa ccctgctttc
    49141 aaacttgctt cgataaattg atccaggctg ccgtccagca cggcctgcgt gttgcgggtt
    49201 tctaccccgg tgcgcagatc tttaatgcgg gagtcatcaa ggacataaga acgaatctgg
    49261 ctgccccagc cgatgtcgga tttattgtct tccatcgcct gcttctcggc atttttcttc
    49321 tgcatctcca gttcataaag cttcgctttc atctgcttca tggcctggtc tttgttcttg
    49381 tgctgggaac ggtcgttctg gcactgggtc acgatcccgg ttgggatgtg agtaatacgc
    49441 accgcagatt cggtacggtt aacgtgctga ccgcccgcgc cggacgcgcg ataaacgtcg
    49501 atgcgcagat ccgccggatt gatttcgata tcgatatcgt catcaacttc cgggtaaaca
    49561 aacgcggagc tgaacgacgt gtggcgacga ccgccggagt caaacgggct cttacgcacc
    49621 aggcggtgaa cgccagtttc tgtacgcagc cagccgtaag cgtaatcgcc ggagattttg
    49681 atcgtcacgg atttaatacc cgccacttca ccttccgact cttcgatgat ttcagttttg
    49741 aaaccacgcg attctgccca gcgcagatac atacgctcaa gcatgctcgc ccagtcctgt
    49801 gcttccgtac cgccagaccc cgcctgaata tcgaggtagc agtcggcgct gtcatattcg
    49861 ccagagaaca tacggcggaa ctcaagctgc gccagttttt cttccagagc gtcgagttca
    49921 gcaacggctt cgttaaaggt ttcttcgtcg tcagcttcta cagccagttc cagcagacca
    49981 gaaacatctt ccagcccctg tttcatttgg tcgagggtgt cgacaacggc ttcgagggaa
    50041 gaacgctctt tacccagggc ctgtgcgcgt tcgggttcgt tccagacatc cggctgttcc
    50101 agctcggcgt ttacttcttc cagacgctct ttcttggcgt cgtagtcaaa gataccccct
    50161 aagaacgtcg gagcgttccg tgaggtcctg aatgcgatta tttaccggat taatttcaaa
    50221 catggtctga tttcttttat tgagctagtc aaaatgcggt gataagagcg ggattgtacc
    50281 caatccacgc tcttttttat agagaagatg acgctaaatt ggccagatat tgtcgatgat
    50341 aatttgcagg ctgcggttgc cgcgaaactc gttgatatcg agcttgtaag tcagttgcac
    50401 ttcgcgcacg ccgttatccg gccagagggc ggtatcgaca ttaaaagcaa taccatccag
    50461 cagcggaccg ccgccgaccg gctcgaccat gactttcaaa tgacgctcgc ccaccagccg
    50521 ctgttgcagc agacggaaat gaccgtcaaa cagcggctcc gggaacatct gcccccacgg
    50581 gccagcatcg cgcagcagct gcgccacttc catagtcatt tcggccgggc ttaacggacc
    50641 gtctgacacc acttcgcctt gcaatagtga aggatccagc cactcggtaa ccagctcgcc
    50701 aaaccgttgt tgaaagagtt cgaatttatc ctcttccagc gacaaacccg ccgccatcgc
    50761 atggccgcca aacttgagca tcatgccggg gtagagtgtg tctaatcgct ccagcgcatc
    50821 acgcatatgc agcccctgaa tggagcgacc ggaacctttc agcgtaccgt cacctgctgg
    50881 cgcaaacgcg ataaccggac ggtgaaaacg ctctttgatg cgcgaagcca gaataccgac
    50941 aacgccctga tgccattcgg ggtggtacat tgccagcccg ccgggtaacg tgtcgcggct
    51001 gcgctccagt ttctcgcaca gggtcagggc ttcaatctgc attccttgtt cgatctcttt
    51061 tcgcgtctgg tttagcgcat cgagttcatt tgccagcacg cgcgcttcgc cgatgttgtc
    51121 gcacagcaac agcgccacac cgacggacat atcgtccagt cgcccggcag cattgagacg
    51181 tggccccagc gcaaaaccta aatcgctggc ggcgagtttt agtgcatcac ggtttgccac
    51241 ttccagcagc gctttaatcc ctggacggca ctttccggca cggatgcgac tcatcccctg
    51301 ccaggtgagg atgcggttat tggcgtccag cggcacgacg tccgccactg ttcccagcgc
    51361 gaccagatcc agcagttctg ccaggttagg aattgcgacg ccgcgctcat caaaccagcc
    51421 ctgatcgcgc aaaaaggtgc gcagcgccag catcagataa aacgccacac ccacgcctgc
    51481 cagtgatttc gacgggaaat tacagtcgcg caagttaggg ttaatgatcg cttccgccgc
    51541 gggtaatgtt tcgcccggca aatggtgatc ggtaacaata accggaatgc ccaacgagcg
    51601 agcgtgttca acgcccgaat gggaggaaat accgttatcc accgtgacaa ttaactgcgc
    51661 gccacgggca tgggcctgat cgaccacttc cgggcttaag ccgtaaccgt cttcgaaacg
    51721 gtttggaacc aagtagtcga tattgctgca accaagcgag cgcatcgcca gcacgcttag
    51781 agccgtgctg gtggcaccgt cggcgtcgaa atcaccgacc acaataatcc gcgtgccttc
    51841 gcgaaaagcg ttgtaaagga tctcaacggc cttttcgacg ccgctcagtt gctgccaggg
    51901 cagcatgcct ttaacactgc gttccagttc ctgcgcgcta cgcacacccc ggctggcata
    51961 taaacggcgc agcaagggag gcaattcagc gggcaagtcg gccgtttcat cgacttctcg
    52021 gcgacgaagt tgtatctgtt gtttcacgcg aattatttac cgctggtcat tttttggtgt
    52081 tcgtcgagga attctttcat gtctttcggc ggctggtaac ccggaacaag tgtgccattg
    52141 ctcagcacaa ctgccggagt accgctaacg ccaagctgga cgccaagtgc gtaatggtcg
    52201 gcaatatcca cgtcgcagct ggctggtgcg acgcttttac ctgccatcac atcatcaaac
    52261 gctttgtttt tatctttcgc acaccagata gctttcattt ctttctctgc atcgctgtcc
    52321 agcccctggc gcgggaaagc aagataacgc acggtgatcc ccagcgcgtt atagtctgcc
    52381 atttgctcat gcagtttgtg gcagtaacca caggtaatat cagtaaacac ggtgatgacg
    52441 tgtttttcct gcggcgcttt ataaacgatc atctcttttt caagcgcatt caactgcttt
    52501 aacagcatct tattggtgac attgaccgga gccgtgccac taacgtcata cattggcccc
    52561 tgaatgatat gtttaccatc atcggtgatg tacaacacac cgctgttagt cagaactgtc
    52621 ttcatgccag ctacgggcgc gggctgaata tcgctgcttt tgatgcccat tttggctaac
    52681 gtttgttgaa ttgccgcgtc atcagcctga acaaagcctg aaaacgctgc caacaaggta
    52741 aataacataa aacctttctt cataaatttt cctgttcttt tcagacatca cgcccgcggg
    52801 tgatgctgtt gatgaagttg tcgcagacgc tcggtagcga catgcgtata aatttgcgtg
    52861 gtggagagat cgctgtggcc cagtagcatc tgcaccacgc gtaaatccgc accatgattt
    52921 aataagtgag tggcaaaagc gtgacgcaac acatgcggtg acagcttttc gctgtcgata
    52981 cccgccagca cggcataatg tttaatgcgg tgccagaagg tctgtcgcgt catctgctgc
    53041 gcacgctggc tgggaaacaa cacgtcaatt gacacaccat tcaacagcca cggacgccca
    53101 tgttccagat aggtttccag ccagtaaacc gcctcttcac ctaacggcac caggcgctct
    53161 ttgttgcctt taccaatgac ccgcactacg ccctgacgca ggctgatatc actcattgtc
    53221 agcccgacca gttcagagac acgcagtccg gtagcataca acacttcaag catggcttta
    53281 tcgcgtagct ccagtggctg atcaattaat ggtgcctgta ataaacgttc gacctgcgct
    53341 tcgcttaaat cttttggcaa acgctggggc aatttcggtg aagcgagatg cgcactggga
    53401 tcatcttcac gaaacttttc gcgataaaga tactggaaca atcggcgcac tgcactcagc
    53461 aaacgcgctg agctggtggc tttatacccg ccctccagcc gttctgccag taatgcctgc
    53521 aaatcgtcac tttgcgccgt cgccagcgtc aacccacggt gatgcaacca ctccaccatc
    53581 attgacagat cgcgacggta agcgttcaac gtattttcag ccagattttt ttccagccac
    53641 agagcatcaa gaaactgctc gatgcgtgcc agatcctgtt tcacttgcgc cccttatggt
    53701 cactcatttg atccattatg ccttattgtg ccgtgactaa agcgattctg atacactagc
    53761 cgcaaaagcc acagcagaat cgagaagctt acgttatgaa tatgggtctt ttttacggtt
    53821 ccagcacctg ttacaccgaa atggcggcag aaaaaatccg cgatattatc ggcccagaac
    53881 tggtgacctt acataacctc aaggacgact ccccgaaatt aatggagcag tacgatgtgc
    53941 tcattctggg tatcccgacc tgggattttg gcgaaatcca ggaagactgg gaagccgtct
    54001 gggatcagct cgacgacctg aacctcgaag gtaaaattgt tgcgctgtat gggcttggcg
    54061 atcaactggg atacggcgag tggttcctcg atgcgctcgg tatgctgcat gacaaactct
    54121 cgaccaaagg cgtgaagttc gtcggctact ggccaacgga aggatatgaa tttaccagcc
    54181 cgaaaccggt gattgctgac gggcaactgt tcgtgggtct ggcgctggat gaaactaacc
    54241 agtatgacct tagcgacgag cgtattcaga gctggtgcga gcaaatcctc aacgaaatgg
    54301 cagagcatta cgcctgatgt cactgacggc ttagcgcata tgctttgccg tcatctttat
    54361 ctttgcgtct cttgttgcaa caaaatccgc cgtaaatccc gccattcagc ttcgtccatg
    54421 ctgtcggctg ccagccataa atgttgccgt ttaccgctgt cagaacgtaa acgcagcatc
    54481 atgccgctct taatcatcca cggcgctttg acgatgctcc actcctgccc ttgccaacgc
    54541 aaacgcccgt ccatcaacag gcgaatttcc ccctggcgag cattaatgcg gcgctggctg
    54601 cgaacgcaat caaacaccac cagcgaaagc aacaccatcc ataacggggt gtaactgagc
    54661 ggccagggca tgagtaaaat aacagcggca accagcccat gaatcagcaa ggaaagccac
    54721 tgtgcgcgcc aggagacgcg caaatcagat tgccacagga ccacgttccc ggttccgtgt
    54781 ctggatgagt cggaccatca tttccagttc tgcatcggcc ggtttaccgt gattcatcag
    54841 ccagttaaac aggtccgggt cgtcgcattc cagcagacga ataaagatgc gtttttcgtc
    54901 atcgcttaag ctgtcgtact catgttcgaa aaacggcatg attgaaatat cgagttcgcg
    54961 cataccacgg cggcatgccc aatgaatgcg ggctttgttg ttaatgtcca tcttcttcct
    55021 gtctcacgaa aatccagtac ccggctattg taacgtgttt ttcgacttct tttgcgggaa
    55081 tatcagtaaa cacaatcgcg atcgcgaaat taattcgcaa caattcaagg gcttcatttt
    55141 tttggaagtc gcctcgcaaa gcatatcaga aggcacagat cgcgtagtga aagcacttgc
    55201 attgcctcat agctctttta ccattagtca ttaatacgcc gttaagcaac tcaggactct
    55261 attatggctt ttacaccttt tcctccccgt cagcctacgg cttctgcccg tttaccgctg
    55321 acgctgatga cgcttgatga ctgggcgctt gccaccatta ctggcgcgga cagcgaaaaa
    55381 tatatgcagg gtcaggtgac agcagatgtc agccagatga cagaagatca gcacctgctc
    55441 gccgcccatt gcgacgccaa aggcaaaatg tggagcaatt tacgtctgtt ccgcgacggc
    55501 gatggctttg cgtggattga acggcgcagc gtgcgtgaac cgcagctgac tgaactgaaa
    55561 aaatatgcgg tgttctctaa agtgaccatc gcgccagacg acgagcgtgt gctgcttggt
    55621 gttgccggtt ttcaggcgcg cgccgcgctg gcaaatatct ttagtgaact gccttcgaaa
    55681 gaaaaacagg tagttaaaga aggcgcgacc actttgctat ggtttgaaca cccggcagaa
    55741 cgtttcctga tcgtaaccga tgaagccact gccaatatgc tgaccgataa actgcgcggt
    55801 gaagcggaac tgaacaatag ccaacagtgg ctggcattaa acattgaagc gggtttcccg
    55861 gtgattgatg ccgccaacag cgggcagttt atcccacagg cgaccaacct ccaggcgctg
    55921 ggcggcatta gctttaaaaa aggctgctat accggacaag agatggtggc gcgagctaaa
    55981 ttccgtggtg ccaacaaacg cgcgctctgg ttgctgacgg gtagcgccag ccgactgccg
    56041 gaagccggtg aagacttaga gctgaaaatg ggcgagaact ggcgtcgtac cggaaccgtg
    56101 ctggctgcgg taaaactgga agatggtcag gtcgtggtac aggtcgtcat gaataacgat
    56161 atggaaccgg acagcatctt ccgcgtgcgt gacgatgcga atacattgca tatcgagccg
    56221 ctgccgtatt cactcgaaga gtaaatctct gtgtcgcatc atgcatttat cgcctgatgc
    56281 gacaagggtg tgtcttttca ggcctacaaa ggcacaccca ttacgcctgc ccaatatata
    56341 aatagatcgc cagaaagtgg cacacgctgc cgccgagcac aaagccgtgc cagatggcat
    56401 ggttgtaggg aatgcgtttg cagacgtaga aaatcacccc gagcgagtaa accacgccgc
    56461 ctacagccag taaggtaacg ctgcccgccg caagcttaat tgccatttca taaatcacca
    56521 ccagcgacag ccagcccatc gccagatagg tcaccagaga taaaattttg aatcggtgcg
    56581 cgatggtcag tttgaacaga atccccagca atgccaggct ccagataaca atcatcaacc
    56641 cgcgcgctaa cggagaatcc agccccacca gcaaaaacgg cgtgtaggtt ccggcaatca
    56701 ataggtaaat agcgcaatgg tcaaatttct tcagccacat ttttgcccgt tgatgaggaa
    56761 tggcgtgata gagcgtcgaa gcgaggaaca gcaggatcat actgccgcca tagaggctgt
    56821 aactggttat cgctgtggcg ctggcattga gatccaccgc ctgaaccagt agcaacacca
    56881 gcccaacgat accaaacacc aacccaatgc cgtgactgac gctgttggca atttcctctg
    56941 ccagcgaata tccctgctta atgaggggct tctgaaccat aacttactcc ggagaaacgt
    57001 acacgcacat gtatacctct ccagcgtaac tgagaatagt tccagtgaac acctgttagc
    57061 taaaataaat tctgatttga gactaatctc cgaaaaatca tgaaattaaa tgcgaaattt
    57121 caactaacag gcgtgagttc aatttaaaga catttaaatt caatcacata aaactgtgtc
    57181 tggccgggat agatatcggc aatgaccttt ttcagatcag tcagggtcat attttcctgc
    57241 tctgcatgtt tttccgtcag tgtatccagc gttacggttg aggttgcggt gacttcaatc
    57301 gtgcaaaaat aaccgtcgtc ttcaaaacgc ccgacacgaa gcacatcacc cgttttgaag
    57361 tgcgattcag actcgtcgcg gatggtgatg gttttacgcc cagccagaat gtcatcctgg
    57421 aaacgttgaa aaaaagtgat gtcgtttggc tgcatggtac tatttcctgt aaaaattgac
    57481 tcatctggag cctatgatag tgaaaaaact caccttaccg aaagatttct tatggggcgg
    57541 cgcagttgcc gctcatcagg tcgaaggcgg ctggaacaaa ggcggcaaag ggccgagcat
    57601 ttgtgacgtt ctgaccggtg gcgcacacgg cgtgccgcgc gaaatcacca aagaagtgtt
    57661 gccaggaaaa tactatccga accatgaagc cgttgatttt tatggtcatt acaaggaaga
    57721 catcaagcta tttgccgaaa tgggcttcaa atgttttcgt acatccatcg cctggacgcg
    57781 catttttcca aaaggcgatg aagctcagcc aaacgaagaa gggctgaagt tctacgatga
    57841 tatgttcgat gaactgctga aatacaacat cgaaccggtg atcaccctct cccactttga
    57901 aatgccgctg catctggtgc agcaatacgg tagctggacc aaccgtaaag tggttgattt
    57961 ctttgtccgt ttcgcagaag tggtatttga acgctataag cataaagtca aatactggat
    58021 gaccttcaac gaaattaaca accagcgtaa ctggcgtgca ccgctgttcg gttactgctg
    58081 ctccggcgtg gtgtataccg agcatgaaaa cccggaagag acgatgtacc aggtgctgca
    58141 tcaccagttt gtcgccagcg ccctggcggt gaaagccgcg cgtcgcatta acccggaaat
    58201 gaaagtcggc tgtatgctgg cgatggtgcc gctttaccct tactcctgta atccggacga
    58261 tgtgatgttc gcccaggagt cgatgcgcga acgctacgtc tttaccgatg tgcaactgcg
    58321 tggctattac ccgtcctatg tgttgcacga gtgggagcgt cgcggattta acatcaaaat
    58381 ggaagacggc gatctcgatg tgctgcgcga aggcacctgc gattatctcg gtttcagcta
    58441 ttacatgacc aacgcggtga aggccgaagg cggcaccggc gatgcgatct ctggttttga
    58501 aggcagcgta ccaaacccgt atgttaaagc gtctgactgg ggctggcaga ttgatcctgt
    58561 gggtctgcgc tatgcacttt gcgaactgta tgagcgttat cagaagccgc tgtttattgt
    58621 cgaaaacggt tttggcgctt atgacaaagt ggaagaagat ggcagcatca acgatgacta
    58681 ccgcattgac tacctgcgcg cccatattga agagatgaaa aaagcggtga cttacgatgg
    58741 cgtggatctg atgggctaca caccgtgggg ctgcatcgat tgcgtgtcgt tcactaccgg
    58801 gcagtacagc aaacgctacg gctttatcta tgtgaataaa catgacgacg gtactggcga
    58861 tatgtcgcgt tcacgtaaga agagctttaa ctggtacaaa gaagtgattg ccagcaacgg
    58921 cgagaagctt taattacccg atgtgcctga tgcgccccgc tcatcaggcc tggcgctcct
    58981 gtcatatatt gaactccagg agtttatatt tacgcttcat ccgacacaca aagcgcactt
    59041 tattaacaag ctaaaaaggc gccgaagcgc ctttagaaga tagtcgaatc agtgaattac
    59101 tggtattcgc taatcggtac gcaggagcag aacaggttac ggtcgccgta aacatcatcc
    59161 agacgtttca ctgtcggcca gtatttgtct gccacgcctg ccgggaatac cgcaacttca
    59221 cggctgtacg gatgcgccca ctcggcgacc agttcgctct gaatgtgcgg cgcgttcacc
    59281 agcgggttat cttccagcgg ccagacaccg gctttcacct ggtcaatttc tgcgcggata
    59341 gccagcatcg cgtcgataaa gcgatccagt tccactttgc tttcagattc agtcggttca
    59401 accatcagcg tacccgccac cgggaacgac atggtcggcg cgtggaaacc gtagtcgatc
    59461 agacgcttgg caatatccag ttcgctgatg ccggtttctt ctttcagcgg gcgaatatcg
    59521 agaatacatt cgtgcgccac gcgaccgtcg cgaccggtat acagcaccgg gaaggcatcc
    59581 tgcaggcggc tggcaatata gttggcgtta agaattgcca cctggcttgc ttttttcagc
    59641 ccttctgcgc ccatcatgcg gatgtacatc cagctgattg gcagaatgga tgcgctaccg
    59701 aacggtgccg cagaaaccgc gccctgacgg gttaacatgc cttcaatttg taccacgcta
    59761 tgacctggca caaacggtgc cagatgcgct ttcacgccga tcggtcccat accagggccg
    59821 ccgccaccgt gcggaatgca gaaagtttta tgcaggttaa ggtgcgagac gtccgcgcca
    59881 ataaagcccg gtgaggtgat gccgacctgg gcgttcatgt tcgcgccatc aaggtaaacc
    59941 tgaccgccga actgatgcac gacttcacac acttcacgga tcgtttcttc atacacgccg
    60001 tgggtggaag gataggtcac catgatacag gagaggttat cgcccgcctg ttccgctttc
    60061 gcgcgcagat cagtcagatc gatgttgccg tttttatcac acgccacaac caccacctgc
    60121 attcctgcca tatgtgcaga agcggggtta gttccgtgcg cagaagccgg gatcaggcag
    60181 atatcacgat gcccttcgtt gcggctttca tgataatgac gaatcgccag caggcccgcg
    60241 tattcgccct gtgcgccaga gttcggctgc atacaaacgg cgtcgtaacc ggtcagtttc
    60301 accagccagt cagccagctg cgcaatcatc tgctgataac cttcggcctg ctccggcggg
    60361 cagaacgggt gcagttcggc aaattccggc caggtgattg ggatcatctc ggcggcggcg
    60421 ttcagtttca tggtgcagga acccagcggg atcatcgcct gattcagcgc cagatcttta
    60481 cgctccagcg agtgcatata gcgcatcatt tcggtttcgc tgtggtagcg attaaacacc
    60541 ggatgggtga ggatttcgtc gtcgcgcagc atcgcaggct gaatagagcg gctgtcgtga
    60601 gccacgtctt tgtccagcgt gtcgatgtcc aggccgtggt tatcccccag cagtacgctg
    60661 aaaagctgca tcacgttttc acgcgtggtg gtttcatcaa gggtgatccc aaccgcgttc
    60721 aggatatcgc tacgcaggtt gatttcagcc gcttcggcac gcgccagtac gcccgccttg
    60781 tctgccactt ccacacacaa agtgtcgaaa tagtgcgcat gacgcagttt cagacctttt
    60841 tgttgcaggc ccgccgccag gatatcggtc agacggtgga tgcggttagc gatacgtttc
    60901 aggccaaccg ggccgtgata aacggcatac aggctggcga tgtttgccag cagtacctgg
    60961 gaagtacaaa tgttggagtt cgctttctca cggcgaatat gttgctcgcg agtctgcatc
    61021 gccatgcgca gagcggtatt gccagctgca tctttcgata caccgataat acggcccggc
    61081 attgagcgtt tgtattcatc tttcgccgca aagaatgctg cgtgtgggcc accgtagccc
    61141 atcggcacac cgaagcgttg cgccgaacca aagacaatat ccgcgccctg tttacccggc
    61201 gcagttaaca gcaccagcgc cattatatcg gcggcgacac tgaccacaat tttgcgtgat
    61261 ttcagttcgc tgatgagcgc agtgtagtcg tggatttcac cggttgtgcc aacctgctgt
    61321 aacagcacgc cgaagacgtc ctgatggtcg agcacttttt gcgcgtcatc gacaatcact
    61381 tcaaaaccaa aggtttcagc acgagtacgg accacatcga gcgtttgcgg atgaacgtca
    61441 gaagcaacga agaagcggtt ggcatttttc agtttgctga cgcgtttcgc catcgccatt
    61501 gcttcggcgg cagcagtggc ctcgtccaga agagaggcag aggccatatc cagcccggtc
    61561 aaatccagcg ttacctgctg gaagttgagc agtgcttcaa ggcggccctg ggagacttca
    61621 ggttgatacg gagtatacgc ggtataccag cccggatttt ccagcatgtt acgcagaata
    61681 accggcggta gctgcacggc ggtgtaaccc atgccgatgt aagacgtgaa gcgtttattg
    61741 cgactggcaa tagccttgag ttccgccagc gcggcgtatt cggtcgccgg tgcgccaacc
    61801 tgcggcgggg tcgcaagctg aatatctttc ggcacaatct ggccggtcag cgcgtttaac
    61861 gattgtgcgc caacggcatt cagcatttct tgctgttgcg cggcgtccgg tccgatatgg
    61921 cgttcaataa aagcgccgct gttttcaagc tggcttaacg tctgtgtcat gagcgatggt
    61981 tcctgaaacg tgcagtgaat tgtgaacctc tctccttgcg aagagagtga gggtgaggcg
    62041 taaaagttcc tcaccctgat cctctcccgc agaagaggaa taaagccgtt actcgtcttc
    62101 taacaatgct tcgtatgcgg tcgcatccag cagtgattcc agttcgcttt catcgctggc
    62161 tttgatttta aagatccagc cgcctgcata cggttcgctg ttcaccagtt ccggggaatc
    62221 gctcagtgcg tcgtttaccg ccacgatttc accgcttact ggcgcataaa tgtctgacgc
    62281 cgcttttact gattcggcaa ccgcgcagtc atcgcccgcg ctaaccgttg cgcccacttc
    62341 cggcaggtca acaaacacca tatcgcctaa cagctcctgg gcatgttcgg tgataccaac
    62401 ggtgtaagtg ccgccggctt ctttacgcag ccattcgtgt tctttgctgt atttcagttc
    62461 tgctggtacg ttgctcatca atcaatctcc aaaaaagtaa atcacgcgac ggctttaccg
    62521 ttacgcacaa aaacaggttt cgtcacttta accggcattt cacggttgcg aatttgtaca
    62581 atcgctgttt caccaatacc ttccggcacg cgcgccagcg caatgctgta acccagcgtc
    62641 ggggagaaag taccgctggt gataatgcct tcatgctggt tgccctgcgc atcggtaaag
    62701 cgtaccggca gttcattacg cagcacgcct ttttcggtca tcaccagacc aaccagtttt
    62761 tctgtgccat gctcacgctg cacttccagg gcttcacgac cgatgaagtc acgatctgcc
    62821 ggttcccagg cgatagtcca gcccatattg gcggctaaag gagagatggt ttcgtccatc
    62881 tcctgactat aaagattcat acccgcttcc agacgcagcg tgtcacgcgc gcccaggccg
    62941 catggcttaa cgcccgcttc caccagcgcg cgccagaaat ccgccgcttt ttcattgggc
    63001 agcgcaattt catagcccgc ttcaccggta taaccagtgg tggcaataaa cagatcgccc
    63061 gcctgcacgc caaagaacgg tttcatccct tccaccgcct gacgctgggc gtcattaaac
    63121 agtgtggcag cttttgcctg cgcattcggc ccttgtacgg cgatcatgga aaggtcatca
    63181 cgaacggtaa tttcgatgcc gaaaggttca gcgtgttggg taatccagga gaggtctttt
    63241 tcgcgggtgg cggagttaac aacgaggcgg aagaaatctt cagtaaagta gtagacgatg
    63301 aggtcatcta tcacaccgcc agaggcattc aacatccccg agtaaagggc tttgccgctt
    63361 ttggtgagtt tcgccacatc gttcgccagc agataacgca gaaactcccg agtgcggctg
    63421 ccgcgaagat cgacgatggt catatgtgac acatcaaaca ttccggcatc ggtacgtacc
    63481 gcatgatgtt cgtcgatttg cgaaccgtaa tgcagcggca tcatccagcc gtggaaatcc
    63541 accatgcgag cgccgcaaag cgtgtgttgt tcgtacaaag gagtctgttg tgccatcttg
    63601 tcctcattga ataagcgggg ctgacaactt ttccatggcg aaattatcac cacgaaaccc
    63661 agcatcggag ccactcccgg tccccaacgc aatcgttctc ttttgcctga acttaccacc
    63721 gaaacagact gttaaccata aggtaaaatt gatcatcaca ttagcttatg gttaaaaaat
    63781 gcaaaaatcg caacagaata aaaaaccaaa aaacacatca gtttctatac aaagatgatg
    63841 tgatgagaaa gttaatttga ataagacaat attaagcgct aaaaaaatgt caaaaaacac
    63901 taaatcaaaa atccataaca ttagaaaata taatgcgaaa acggaggtta aattagttta
    63961 tttcaaatga ggaaaatctc ccggcgaaaa aaccgggaga tgaaagtgtg atgggtatca
    64021 aataaacagc agaagagaaa tatttaacgc agccattcag gcaaatcgtt taatcccatt
    64081 gcctgacgaa taagttgtgg cttaacgcca ggaagcgtat cagccagttt cagaccaata
    64141 tcacgcagca attttttcgc cggattggta ccggaaaaca gatcgcggaa tccctgcata
    64201 ccagccagca tcagcgccgc actgtgctta cggctacgct catagcgacg caggtaaatg
    64261 tactgcccga tgtctttacc ctgacgatgc aaccgtttca gttcagcaat cagctctgca
    64321 gcatccataa agccgagatt taccccctgc cccgccagcg ggtgaatagt atgcgcggcg
    64381 tcgcccacca gcgccagacg gtgtgcggca aactggcgcg cataacgccc cgttagcggg
    64441 aacaccaggc gctcactttc aaccttgcat aagcccaggc gattatcaaa agcgatattt
    64501 aacgcgcgat taaattcgtc ttcgcctgcc tgctgcatcc gctgcgcttc ctctggcgac
    64561 agtgaccaga caatcgagca aagatgcgga tcgctaagcg gcaaaaaggc cagaatgcct
    64621 tcgccatgaa aaacctgccg cgccaccgca tcatgcggtt cttccgtgcg aatggtcgct
    64681 accagcgcgt gatgctgata atcccagaaa gtcagcggaa tatcggcttt gttgcgcaac
    64741 caggaattag cgccgtccgc gccaatcacc agacgcgccg ttaacatact gccatctttc
    64801 aacgtcagga aggtttcatt ttctccccag gcgacctgct gtaattctgc gggggccaac
    64861 agagtgatat ctgacgactg ctgcgctttg ttccacagcg cgtagtgaat caccgagttt
    64921 tcaacgatat gcccaagatg gctatagccc atgctttgat cgtcaaacga aatgtgacca
    64981 aaactgtctt tgtcccacac ttccataccg tgataacaac tggccctacg agagagaatg
    65041 tcctgccaga caccaagacg ggtgagtaat ttttcgctgg cggcattgat agccgaaacg
    65101 cgcagttgtg gtggtgcatc cgccgccaga ggttccggta cgcgctgctc cagtacggca
    65161 acgcgtaagc cgctaccctg caagccgcag gcaaccgcca gccccaccat gccgccgcca
    65221 acaatggcta catcaacact ttgcattgtt tattccttaa aaccgccttc aacgcgccac
    65281 ccacccgagg gtgcgctgcg ccagcacatc gcgtgctggg gtgaataatt ccattgccat
    65341 cagcccgatg ttgcgcccga caaccagcgg cccccaacgg ttggcaaaaa gatgtacaag
    65401 gctgtccgtg acgccgatgg ttgcttcgcg atcgcgctgt cgacgctgct gataacggca
    65461 caatacgccg taatcaccca tgtcttctcc gcgctcctgc gcctgagtca gggtttccgc
    65521 aaggctcatg acatctcgca taccgaggtt gaacccttgc ccggcaatcg ggtgcagagt
    65581 ttgcgccgca ttgcccacca gcacggtacg atgggtaata ggtctggcgg cgcgggttaa
    65641 cgccagcgga taagcactgc gtttaccagc gtgggtaatt ttcccaagtc gccaaccaaa
    65701 ggccgactgg agttcacggc aaaacttctc gtcgctccaa gacagtacct cttcgcgccg
    65761 ttccagtgga tgacaccaga ccagcgaaca gcgtccgtca gacatcggca acatcgccag
    65821 cgggccatat tgtgtaaagc gttcaaaagc gcgcccttca tgcgcaacgg aagtcgcaac
    65881 gttggcaatc acagccagtt gttcgtaagg ctcctgctgc cagtcaacgc cgcacacggt
    65941 ggctaacgct gaatgggtgc catcagcggc taccagcacg cagcccgtca gcgtctcgcc
    66001 actctccagc gtcacttcaa cgtgactctg agtacgggca acgttagcca cgcgatcagg
    66061 acaatgcagc gttacgccag gtgctttgcg cagcaatgca aacagccgtt gcccgacatt
    66121 atgcaattcg acaacctgcc ccagcgccgc cagttggtaa tcttctgcgg cgagggtgac
    66181 gaatccggca tgaccacgat cgctgacatg cacggtagtg atggcggttg cgcaatccgc
    66241 cagagattgc cagacgccga tgcgcgccag ttgctgacag gtacccgccg ccagcgctat
    66301 cgctcgtcca tcaaagcccg gatgagcatg tgactctggc gcagtcgctt caatcaaatg
    66361 taccggcagc gccccgtgac ttaaccggga aatagccagc gccagcgtcg cgcccgccat
    66421 gccgccaccg acgatgatta cgctcattgc tttctcgcag cagccatcaa cgcttcgatt
    66481 tcttccggct ttttcaccac gctggcggtg aggttttcgt tgccggtttc ggtaatcaca
    66541 atgtcgtctt caatacgaat gccgataccg cgatattgtt ctggcacatc cgcatccggc
    66601 gcgatataca gaccaggctc tacggtcagt accatccccg gttccagaat gcgcgagcga
    66661 tcctgaccat aaacgccaac gtcatggaca tccagtccta accagtggct aaggccatgc
    66721 ataaagaaag gacgatgggc gttctgagcg atcagttcat caacatcacc cttcaggatg
    66781 ccgagtttta ccaggccgct aaccatgata cgcaccactt caccagtgac ttcctgcatg
    66841 gaagttcccg gacgatacag gcgcaggctg gtttcaagag actccagcac aatgtcatag
    66901 atttcacgct gagcctgagt gaatttgccg ttgaccggga aggtgcgggt aatgtcgcca
    66961 gcgtaacctt tgtattcaca acccgcgtca atcaacacca gatcaccgtc acgcagttca
    67021 cactcgtttt cggtgtagtg caaaatgcag ccgttttcac cgctgccaac aatggtgtta
    67081 taggaagggt aacgcgcacc gtggcggtta aactcgtggt gaatttcgcc ttccagatgg
    67141 tactcgaaca tccccggacg gcatttttcc atcgcccggg tatgcgccag tgcggtgatt
    67201 tcacccgcgc ggcgaagtac ggcaatctct tctggtgatt tgaacaggcg catttcatga
    67261 acaacaggac gccagtcgat catcgtagcc ggggcagtga gattctgtcg cgagccttta
    67321 cgcagctttt ccagcgcgct gttcacgatt tcatcagcat aggtatattc gccctgagcg
    67381 tgataaacca catccagacc attaagcagt tgataaagtt gctgattgat ttcgctgaat
    67441 gccagtgcgc ggtcaacgcc cagtttctct ggcgcggcat cctggcctaa gcgacgacca
    67501 aaccagatct ctgccgtcag gtcgcgaacg cggttaaaca aaacgctgtg gttatgagtg
    67561 tcgtcgcttt taatcagcac cagcaccgct tccggttcgt taaagccggt gaagtaccag
    67621 aagtcactgt tctgacgata ggggtattcg ctgtcggcgc tacgtgttac ttctggtgca
    67681 gcaaaaatca gcgcggcgct gccgggttgc atttgctcca ccagggcctg acggcgacgc
    67741 tgaaactctt gccgggatat ctcactcata acactctcct tacgtttttt gtttttagtg
    67801 tagagtcggt ttttgtactt ctggcgcggt cggttgcgga tgagtaaagg tgtcgtggca
    67861 taacagcgcg gcaacacgaa cgtattcgat gatctcttca agcgacattt caagctcttc
    67921 ctgatcttcg tcttcgtcgt agcccagctg cgcgatgtta cgcagatcgt cgatggcttc
    67981 accggtttct ccggtcactt tgtccagctt cggttgcgta acgccaagac caagcaggaa
    68041 gtgattgacc caacctgcca gcgcatcagc ccgatcgaaa acgctgacat catcgccatc
    68101 aggcagataa agctgaaaaa ggaagccgtc atcctgcagg gcatcgctgg tggcagagtg
    68161 cattttacgc agtgcctgtg ccagctcatg accgaaagcc atgccttcgt tcgtcaggtc
    68221 gtgaagtagc ggcagccatg agctgtcatc gttaccgcca catatcatcc cgctgattaa
    68281 accatgcatc tcagcagggg tcagacccgt cccttgttgg ttcagatact ggttcatttc
    68341 gttgtaacca ggcatttcgt tctgtataga cataagcatt cgtcgtcaaa gggaggaata
    68401 ttcatgatat gctaccactt tgggccctgg tggaccagaa aagggcttgt ctcttctcat
    68461 cagggtagct atagtgtcgc cccttcgcag accatgggtc taaagacgaa ggcagcgcag
    68521 tcaatcagca ggaaggtggc atgtctgcac aacccgtcga tatccaaatt tttggccgtt
    68581 cactgcgtgt gaactgcccg cctgaccaaa gggatgcgtt gaatcaggca gcggacgatc
    68641 tgaaccaacg gttgcaagat ctgaaagaac gcactagagt cacaaatact gaacagttgg
    68701 tcttcattgc cgcattgaat atcagctatg agttagcgca agaaaaagcg aagactcgtg
    68761 actatgcggc aagtatggaa cagcgtattc ggatgctgca gcagaccata gaacaagcgt
    68821 tatttgaaca aggtcgcatc accgaaaaaa ctaaccaaaa ctttgaatga cacttttcgg
    68881 tttactgtgg tagagtaacc gtgaagacaa aatttctctg agatgttcgc aagcgggcca
    68941 gtcccctgag ccgatatttc ataccacaag aatgtggcgc tccgcggttg gtgagcatgc
    69001 tcggtccgtc cgagaagcct taaaactgcg acgacacatt caccttgaac caagggttca
    69061 agggttacag cctgcggcgg catctcggag attcccttct tatctggcac cagccatgac
    69121 gcaactacca gaactcccac tgacattatc ccgacaagaa atccgcaaaa tgattcggca
    69181 acgtcgtcgt gcgttaacgc cggaacaaca gcaggaaatg ggtcaacaag ccgctacccg
    69241 gatgatgact tatcccccgg tggtgatggc acatacggtc gctgtattcc tctcttttga
    69301 tggcgaactc gacaccctgc cactcataga acaactctgg cgcgccggta agcgcgtata
    69361 tcttccagtt ttgcatccct tcagtgccgg taatttgctg ttcctgaatt accatccgca
    69421 aagcgaactg gtgatgaaca ggttgaagat ccatgagccc aaactggatg tgcgtgacgt
    69481 gctacccctt tcccgattag acgtgttgat cacaccgctg gtcgcctttg atgagtacgg
    69541 tcagcgcctg ggaatgggcg gtggttttta tgatcggacc ttacaaaact ggcagcacta
    69601 taaaatgcaa ccggtgggtt atgcgcatga ttgtcagttg gtggaaaaac tccccgttga
    69661 agagtgggat atccctcttc ctgcggtggt tacaccgtcg aaagtctggg agtggtgagg
    69721 gcgacacacc tgcatcgccc tgattgacat cgttgattct ttaacctaat ttagtgagta
    69781 agggtaaggg aggatgactc ctcccctgag actgactgtt aataagctgg gaaacttatg
    69841 agtaacagta caatcagtat gatgacaagt cgcatcataa cccttctcct tcaagccctc
    69901 gcttcggtga gggctttacc gttacagccc catgctgccc tgccatcgta aatccccatt
    69961 aaataaacac aacgcattga tctgactttg atttattttc tggagcagac tcgcaaagta
    70021 gaatgcgcaa tgcggcaacg gtgtggagaa gggataaaaa aacgggcaag tcagtgacct
    70081 gcccgttgat tttcagataa ggggatttag tacagcagac gggcgcgaat ggtacccgga
    70141 atagctttca ttgcctgcag cgctttttcg gcaacgtctt cgtcggcttc aatatcaata
    70201 actacataac ccatctgggc ggaagtttgc agatattgcg cggcgatgtt gacgccctgc
    70261 tcggcaaaaa ttttgttgag cgcagtcagc acacccggac ggttttcgtg gatgtgcatc
    70321 agacgacgac caccgtgcag tggcagcgag acttccggga agttcaccgc agagagcgtt
    70381 gaaccattat cagaatactt gatcaattta cccgcaactt ccagaccgat attctcctgc
    70441 gcttcctgag tcgaaccacc aatgtgtggc gtcagaagga cattgtcgaa ttcacacagc
    70501 ggagaggtaa atggatcgct attggtcgcc ggttccgtcg ggaatacgtc gattgccgcc
    70561 cctgccagat gtttgctcgc cagcgcgtca cacagcgctg gaatatccac cacagtaccg
    70621 cgcgaagcat taatcagcag cgagccgggt ttcattagcg aaatctcttt cgcgcccatc
    70681 atatttttgg tggacggatt ctctggtaca tgcagactca ccacatcgct catattcagc
    70741 aggtcagaaa gatgctgtac ctgagtggcg ttgcccagcg gcagtttgtt ttcaatatca
    70801 taaaagtaaa catacattcc cagcgattca gccagaatgc ccaattgcgt accaatatga
    70861 ccgtagccga tgatacccag ctttttgccg cgcgcttcaa aagaacccgc cgccagtttg
    70921 ttccacacgc cacgatgcgc tttagcattg gcttccggca cgccgcgcaa tagcagcagc
    70981 agttcgccaa tcaccagctc cgcaacagag cgcgtatttg agaacggtgc gttaaatacc
    71041 gggatcccgc gttttgccgc cgcatccaga tcaacctggt ttgttccgat acagaaacag
    71101 ccaatagcga ccagtttttc tgcggcgttg atcacgtctt cagccagatg ggtacgggat
    71161 cgcaggccga tgaagtgggc atcgcggatg gattctttta attgttcatc atccagcgcg
    71221 cctttgtgaa attcgatgtt ggtgtaaccc gctgcacgaa ggctttccag cgccttttgg
    71281 tgcacgcctt ctaccagcag aaacttaatc ttgtctttct ccagcgatac ctttgccatt
    71341 tacccaatcc tgtcttttga aatgttgtgt gcggatttgc atccgccttt caacatatca
    71401 aaaaataata ttgcggcaat atgaacgttt gcgtcacgat attgaggaaa tatcacgcaa
    71461 aggtatttct tggatgaata tgctggttta gagggtttat ttagagcaat cgacaatagc
    71521 caggtaaaaa cgtgacacat gtcaccaaat ttaatgaaga gaattttttt aacgggggag
    71581 gttcccccgt cagatcattt cacaatggtt ttgacaccgt caggtgtgcc aatcagcgca
    71641 acgtccgcgc cacggttagc aaacaagcca acagtcacca cgccaggaat cgcatttatg
    71701 gcgttttcca tcgctatcgg gtcaaggatt tccatgccgt ggacgtcgag gatcacgttg
    71761 ccgttatcgg tcaccacgcc ctgacggtat tccggacgac cgcccagttt caccagctgg
    71821 cgcgccactg cactacgtgc catcgggata acttctactg gcagcgggaa tttgcccaga
    71881 atatcaacct gcttggaggc gtcggcaata cagataaatt tttccgcaac cgaagcaatg
    71941 attttttcac gggtcagcgc cgcgccgccg cctttgatca tttgcatgtg gccgttgatt
    72001 tcatctgcgc catcaacgta gatgccaagg ctgtcgactt cgttgagatc aaaaacgtga
    72061 atgccgaggc ttttcagttt ttcagtggaa gcatctgaac tggaaacggc cccttcaatc
    72121 tggcctttca tcgtgccgag cgcgtcaata aagtgtgcgg cggtggaacc tgtacctaca
    72181 ccaacaatgg tgccgggctg aacatactga agtgccgccc atcctactgc ttttttcaat
    72241 tcatcctgcg tcatgatcgt ttcgcctgtg gtatgaaatt tcacacgcat tatatacaaa
    72301 aaaagcgatt cagaccccgt tggcaagccg ggtggttaac tcatccataa aatatcgcgc
    72361 aatggcgggc atcccctttc gccccgcaaa taaagcatac aacggtctgg gtatgccgct
    72421 ccacgatgcg aacagaggca ataacgccac gccattcaac acaaccaggg cgcgaactcg
    72481 ctgagcgaaa tgcattagcg caaatccctg atcttttaag ctgcgcgtta accactgata
    72541 caaatgttac ccgccaacga cttcggtata tgcaacctga cacaaaattg tgtcatagtg
    72601 caggaaaaag catttaccag gagcagacaa cagcaatgaa acgcccggac tacagaacat
    72661 tacaggcact ggatgcggtg atacgtgaac gaggatttga gcgcgcggca caaaagctgt
    72721 gcattacaca atcagccgtc tcacagcgca ttaagcaact ggaaaatatg ttcgggcagc
    72781 cgctgttggt gcgtaccgta ccaccgcgcc cgacagaaca agggcaaaaa ctgctggcac
    72841 tgctacgcca ggtggagtta ctggaagaag agtggctggg cgatgaacaa accggttcaa
    72901 ctccgctgct gctttcactg gcggtcaacg ctgacagtct ggcgacgtgg ttgcttccgg
    72961 cgctggctcc tgtgttagct gattcgccta tccgcctcaa cttgcaggta gaagatgaaa
    73021 cccgcactca ggaacgtctg cgccgcggcg aagtggtcgg cgcggtgagt attcaacatc
    73081 aggcgctgcc gagttgcctt gtcgataagc tcggtgcgct cgactatctg ttcgtcagct
    73141 caaaaccgtt tgccgaaaaa tatttcccta acggcgtaac gcgttcggca ttattgaaag
    73201 cacctgtggt cgcttttgac catcttgacg atatgcacca ggcctttttg cagcaaaact
    73261 tcgatctgcc tcctggcagc gtaccctgcc atatcgttaa ttcttcggaa gcgttcgtgc
    73321 aacttgctcg tcagggcacc acctgctgta tgatcccgca cctgcaaatc gagaaagagc
    73381 tggccagcgg tgaactgatt gacttaacgc cagggctatt tcaacgacgg atgctctact
    73441 ggcaccgctt tgctcctgaa agccgcatga tgcgtaaagt cactgatgcg ttgctcgatt
    73501 atggtcacaa agtccttcgt caggattaat ccatcaaata atgcctgata gcacatatca
    73561 ggcgttatcc tcacttcttt ttgtattcct tgaatcacat cacaaaatag acaaatctca
    73621 ggcggcaaaa aacgacgtct gaatgcattt tttttgctgg cgacaaaccc acgtaaaaag
    73681 ctcaccgtaa gcgcaaatac cctcattttg attgcgtttt acggagcaaa taatgtctaa
    73741 ggtgcaggag tggcaacagc ttgccaacaa ggaattgagc cgtcgggaga aaactgtcga
    73801 ctcgctggtt cagcaaaccg cggaagggat cgccatcaag ccgctgtata ccgaagccga
    73861 tctcgataat ctggaggtga caggtaccct tcctggtttg cctccctacg ttcgtggccc
    73921 gcgtgccact atgtataccg cccaaccgtg gaccatccgt cagtatgctg gtttttcaac
    73981 agcaaaagag tccaacgctt tttatcgccg taacctggcc gccgggcaaa aaggtctttc
    74041 cgttgcgttt gaccttgcca cccaccgagg ctacgactcc gataacccac gcgtggcggg
    74101 cgacgtcggc aaagcgggcg ttgctatcga caccgtggaa gatatgaaag tcctgttcga
    74161 ccagatcccg ctggataaaa tgtcggtttc gatgaccatg aatggcgcag tactaccagt
    74221 actggcgttt tatattgtcg ccgcagaaga gcaaggtgtt acacccgata aactgaccgg
    74281 caccattcaa aacgatatcc tcaaagagta tctttgccgc aacacctata tttacccgcc
    74341 aaaaccgtca atgcgtatta tcgcagacat catcgcctgg tgttccggca acatgccgcg
    74401 atttaatacc atcagtatca gcggttacca catgggtgaa gcgggtgcca actgcgtgca
    74461 gcaggtagca tttacgctcg ccgatgggat tgagtacatc aaagcagcaa tctccgccgg
    74521 gctgaaaatt gatgacttcg ctcctcgtct gtcgttcttc ttcggcattg gcatggatct
    74581 gtttatgaac gtcgccatgt tgcgtgcggc acgttattta tggagcgaag cggtcagtgg
    74641 atttggcgca caggatccga aatcactggc gctgcgtacc cactgccaga cctcaggctg
    74701 gagcctgact gaacaggatc cgtataacaa cgttatccgc accaccattg aagcactggc
    74761 tgcaacgctg ggcggtactc agtcactgca taccaacgcc tttgatgaag cgcttggttt
    74821 gcctaccgat ttctcagcac gcattgcccg caatacccag atcatcattc aggaagaatc
    74881 agaactctgc cgcaccgtcg atccactggc cggatcctat tacattgagt cgctgaccga
    74941 tcaaatcgtc aaacaagcca gagctattat ccaacagatc gacgaagccg gtggcatggc
    75001 gaaagcgatc gaagcaggtc tgccaaaacg aatgatcgaa gaggcctcag cgcgcgaaca
    75061 gtcgctgatc gaccagggca agcgtgtcat cgttggtgtc aacaagtaca aactggatca
    75121 cgaagacgaa accgatgtac ttgagatcga caacgtgatg gtgcgtaacg agcaaattgc
    75181 ttcgctggaa cgcattcgcg ccacccgtga tgatgccgcc gtaaccgccg cgttgaacgc
    75241 cctgactcac gccgcacagc ataacgaaaa cctgctggct gccgctgtta atgctgctcg
    75301 cgttcgcgcc accctgggtg aaatttccga tgcgctggaa gccgctttcg accgttatct
    75361 ggtgccaagc cagtgtgtta ccggcgtgat tgcgcaaagc tatcatcagt ctgagaaatc
    75421 ggccgccgag ttcgatgcca ttgttgcgca aacggagcag ttccttgccg acaatggtcg
    75481 tcgcccgcgc attctgatcg ccaaaatggg ccaggatggg cacgatcgcg gcgcgaaagt
    75541 gatcgccagc gcctattccg atctcggttt cgacgtagat ttaagcccga tgttctctac
    75601 acctgaagag atcgcccgcc tggccgtaga aaacgacgtt cacgtagtgg gcgcatcctc
    75661 actggctgcc ggtcataaaa cgctgatccc ggaactggtc gaagcgctga aaaaatgggg
    75721 acgcaaagat atctgcgtgg tcgcgggtgg cgtcattccg ccgcaggatt acgccttcct
    75781 gcaagagcgc ggcgtggcgg cgatttatgg tccaggtaca cctatgctcg acagcgtgcg
    75841 cgacgtactg aatctgataa gccagcatca tgattaatga agccaagcta gcagaaagta
    75901 ttcgccgctt acgtcagggt gagcgtgcca cgctcgccca ggccatgacg ttggtggaaa
    75961 gccgtcaccc gcgtcatcag gcgctaagta cacagctgct tgacgccatt atgccgttct
    76021 gtggtaacgc cctgcgactg ggcgttaccg gcacacccgg cgcggggaaa agtacctttc
    76081 ttgaagcctt tggcatgttg ttgattcgtg agggattaaa ggttgcggtt attgcggtcg
    76141 atcccagcag cccggtcact ggcggtagca ttctcgggga taaaacccgc atgaatgacc
    76201 ttgcgcgtgc cgaggcggcg tttattcgcc cggtaccctc ctccggccat ctgggcggtg
    76261 ccagtcagcg agcgcgggaa ttaatgctgt tatgcgaagc ggcgggttat gacgtagtga
    76321 ttgtcgaaac ggttggcgtc gggcagtcag aaacggaagt cgcccgcatg gtggactgtt
    76381 ttatctcgtt gcaaattgcc ggtggcggcg atgatctgca gggcattaaa aaggggctga
    76441 tggaagtggc agatctgatc gttatcaaca aagacgatgg cgataaccat accaatgtcg
    76501 ccattgcccg gcatatgtac gagagtgccc tgcatattct gcgacgtaaa tacgacgaat
    76561 ggcagccacg ggttctgact tgtagcgcac tggaaaaacg tggaatcgat gagatctggc
    76621 acgccatcat cgacttcaaa accgcgctaa ctgccagtgg tcgattacaa caagtgcggc
    76681 aacaacaatc ggtggaatgg ctgcgtaagc agaccgaaga agaagtactg aatcgcctgt
    76741 tcgcgaatga agatttcgat cgctattacc gccagacgct tttagcagtc aaaaacaata
    76801 cgctctcacc gcgcaccggc ctgcggcagc tcagtgaatt tatccagacg caatattttg
    76861 attaaaggaa tttttatgtc ttatcagtat gtgaacgttg tcactatcaa caaagtggcg
    76921 gtcattgagt ttaactatgg ccgcaaactt aatgccttaa gtaaagtctt tattgatgat
    76981 cttatgcagg cattaagcga tctcaaccgg ccggaaattc gctgtatcat tttgcgcgcg
    77041 ccgagtggtt ccaaagtctt ctccgcaggt cacgatattc acgaactgcc gtctggcggt
    77101 cgcgatccgc tctcctatga tgatccgttg cgtcaaatca cccgcatgat ccaaaaattc
    77161 ccgaaaccga tcatttcgat ggtggaaggt agtgtctggg gtggcgcatt tgaaatgatc
    77221 atgagttccg atctgatcat cgccgccagt acctcaacct tctcaatgac acctgtaaac
    77281 ctcggcgtcc cgtataacct ggtcggcatt cacaacctga cccgcgacgc gggcttccac
    77341 attgtcaaag agctgatttt taccgcttcg ccaatcacag cccagcgcgc gctggctgtc
    77401 ggcatcctca accatgttgt ggaagtggaa gaactggaag atttcacctt acaaatggcg
    77461 caccacatct ctgagaaagc gccgttagcc attgccgtta tcaaagaaga gctgcgtgta
    77521 ctgggcgaag cgcacaccat gaactccgat gaatttgaac gtattcaggg gatgcgccgc
    77581 gcggtttatg acagcgaaga ttaccaggaa gggatgaacg ctttcctcga aaaacgtaaa
    77641 cctaatttcg ttggtcatta atccctgcga acgaaggagt taaaatggaa actcagtgga
    77701 caaggatgac cgccgatgaa gcggcagaaa ttatccagca taacgacatg gtggcattta
    77761 gcggctttac cccagcgggt tcgccgaaag ccttacccaa cgcgattgcc cgcagagcta
    77821 acgaacagca tgaggccaaa aagccgtatc aaattcgcct gctgacgggg gcgtcaatca
    77881 gcgccgccgc tgacgatgta ctttctgacg ccgatgctgt ttcctggcgt gcgccatatc
    77941 aaacatcgtc cggtttacgt aaaaagatca atcagggcgc ggtgagtttc gttgacctgc
    78001 atttgagcga agtggcgcaa atggtcaatt acggtttctt cggcgacatt gatgttgccg
    78061 tcattgaagc atcggcactg gcaccggatg gtcgagtctg gttaaccagc gggatcggta
    78121 atgcgccgac ctggctgctg cgggcgaaga aagtgatcat tgaactcaat cactatcacg
    78181 atccgcgcgt tgcagaactg gcagatattg tgattcctgg cgcgccaccg cggcgcaata
    78241 gcgtgtcgat cttccatgca atggatcgcg tcggtacccg ctatgtgcaa atcgatccga
    78301 aaaagattgt cgccgtcgtg gaaaccaact tgcctgacgc cggtaatatg ctggataagc
    78361 aaaatcccat gtgccagcag attgccgata acgtggtcac gttcttgttg caggaaatgg
    78421 cgcatgggcg tattccgccg gaatttctgc cgctgcaaag tggcgtaggc aacatcaata
    78481 atgcggtaat ggcgcgtctg ggggaaaatc cggaaattcc tccgtttatg atgtattcgg
    78541 aagtgctaca ggaatcggtg gtgcatttac tggaaacggg caaaatcagc ggggccagcg
    78601 cctccagcct gactatctcg gccgattccc tgcgcaagat ttacgacaat atggattact
    78661 ttgccagccg cattgtgttg cgtccgcagg agatttccaa taacccggaa atcatccgtc
    78721 gtctgggcgt catcgctctg aacgtcggcc tggagtttga tatttacggg catgccaact
    78781 caacgcacgt agccggggtc gatctgatga acggcatcgg tggcagcggt gattttgaac
    78841 gcaacgcgta tctgtcgatc tttatggccc cgtcgattgc taaagaaggc aagatctcaa
    78901 ccgtcgtgcc aatgtgcagc catgttgatc acagcgaaca cagcgtcaaa gtgatcatca
    78961 ccgaacaagg catcgccgat ctgcgtggtc tttccccgct tcaacgcgcc cgcactatca
    79021 ttgataattg tgcacatcct atgtatcggg attatctgca tcgctatctg gaaaatgcgc
    79081 ccggcggaca tattcaccac gatcttagcc acgtcttcga cttacaccgt aatttaattg
    79141 caaccggctc gatgctgggt taatgactcg tcccgtcacg atcttcagca atatgctgaa
    79201 gcatcgtggc ggtataacga tgatttctga ttgaataaag atattcctgc atatacatcg
    79261 gcctgtgcgg cgcatcaaaa tatttcagcg ttgccggatt caccagtcgc caggcaaaat
    79321 gcgggatcac cgtcagaaaa tgtccgcgct ccacggcgct gattttggcc ataaaactgt
    79381 agggacgata aataatggtg ggattgatcc cacaaggacg catattcgca tcaatcattg
    79441 cttcgaagtt agcccggttc tgaaagcgca tttgtagcca gggtagctcc tgaagcaact
    79501 cttgctcttg tcgctcttca taacgacgag aaacgagaaa gcccaatcgc agcggcggta
    79561 gttcactgat agtcaggttt tccagttcct gaactcgtgc agatacatgc tgtggggaga
    79621 taataaaatc taactgacgg tcaaaaagat tatcgataac gccattttcg ctgaactcaa
    79681 ctggctgggc ggtcacgcct tcatacttat ctccgagact aatcaactga tcgaaaataa
    79741 ttgtcggata agtattatct atgccaatca caatttctcg tgaacgacgc cctgagttat
    79801 ggatttcatt atcaatcgca gaaagccgtt gataaaccgg gaacagtttt tgatacagtt
    79861 cctgtccggc tttattgagg ctgatattat tatctttgcg ggtaaatagc gtatagccaa
    79921 tttgttcttc cagcgcggca atgcttttac caaatggcga agctgtcata tggatttttt
    79981 ctgccgcccg ggcaatatta ttggtttgcg ctaataaaat gaaattgcgc atctttttcg
    80041 aaataaaaat gtccataccc cctcccccgc atccttgtcc gcttaaccgt ttcagtcagc
    80101 ctatccttga tgaaaccgcg agcaaagata ggtgattacg tcatggtttt acagaaaatt
    80161 acagaaaaag gaggcaatat cgggtaaagg cattagcccg acgaatacgt cgggctacaa
    80221 atattattgt gctgcaggtg ttttagcggg ttgttgatcc acaggttcta actggaagac
    80281 cacatcgacc tgatcatcaa actgaatagc ggcctgctcg taagtttcct gggcggacac
    80341 cggcgcggca tcggctttca tcatccgcac cattgggctg ggctgatagt tggaaacatg
    80401 gtagcgcaca ctatataccg gccccagttt acgatgaaag ccgttcgcca gttcctgcgc
    80461 ctgatgaatc gcgttatcaa tcgctgcctt acgcgctttg tctttatagg catccggctg
    80521 cgccacgccc agcgatacag aacgaatttc gttaagaccc gccttcagcg cgccatccag
    80581 caaggaattc agtttgtcta actgacggag cgtgacttcc accgttctca cagcacggta
    80641 gcctttcagg atacttttac catcctgata atcataatct ggctgggtgc gtaagttcgc
    80701 tgagctgata tcttttttcg cgatctgatt gagttcaagg aaggaaatgt attgtgcgac
    80761 gcgctcatcc gcctgtttct tggcagtagc ggcatccttc gcggccacgt tgacttcaat
    80821 cgcaagagtg gcaatgtctg gcaccgcatc cacgcttgcc gtaccggagg tgacaatatg
    80881 cggtccatcc ggcaattcgt tagcctgcgc tgccatcccg ctaataccca ttaatgccgc
    80941 cagggcgata actttgaact tcacgttagt ctctccgtgt taattgactt gcctaaaatt
    81001 caggacctct ggaggcaagc ttagcgcctc tgttttattt ttccatcaga tagcgcctaa
    81061 ctgagcaagg cttgtgcatg aacaataccg tctctcgcca gctgcaaggc aataaaccac
    81121 ataacacatc ccactaccag attgataatg cgctgtgatt ttgccgtgcg caggcgcggt
    81181 gccagccagg ctgcgagaat agccagacca aagaaccaca ggaaagaggc gctaattgtc
    81241 ccgagcgcaa accaacgttt tggttccaca tcaagttgcc cgccaagact gcccagtaca
    81301 acaaaagtat ccaggtaaac atgcggattc agccaggtca ctgccagcat ggtggcgata
    81361 attttccatc tgccttgctt caggacttcg gcactggcta actcaatatt actgctcatc
    81421 gctgttttaa aagcgccaaa accataccac agcaagaagg ctacgccgcc ccaggtgacc
    81481 agcgccagca accacggcga ctgcatcaat aacgcgctgc caccaaaaat cccggcgcaa
    81541 atcaggacca aatcgctgat agcgcaaagt aaggcaatca taatgtggta ctgacgacga
    81601 atgccctgat tcatcacaaa agcattttgt ggaccgagcg gtaggatcat agccgcccca
    81661 agtgcaagac cttgaaagta ataagaaaac acgttggata ttccgaatta agtatctgat
    81721 gcagagacta tatcgcggga ttgttattag cggaaattga taatattaat tggcaatcag
    81781 aaaaactaat aagagagagg ccagagagta ttatctggcc tcagcgttga ttacgcagct
    81841 ttgtcttctt tcacccgctt aaagttcaca tccatttgcg ggtacgggaa gctgataccg
    81901 gcggcatcaa attcacgttt aatacgctcc agcacatccc agtacacgtt ttgcagatcg
    81961 ccgctgttgc tccagacgcg gaccacgaaa ttaatcgacg atgcaccaag ttcgttcagg
    82021 cgcacagtca tttcgcgatc tttcaaaatg cgatcttcag actggataat atcggtcagg
    82081 atctgcttaa cctgatcgat atcggaatca tacgccacgc caataataaa ttcgttacgg
    82141 cgaactggct cgcgagagaa gttaataata tttccggcaa taattttacc gttcggaata
    82201 acgataattt taccgtctgc agtacgcatg gtggtggaga aaatctgcac actcagcaca
    82261 gtaccggcta cgccgccgag atcaacatat tctccggcac ggaacgggcg gaacataaca
    82321 agtaacacgc cagcggccag gttagacagt gacccctgca aagccagacc aaccgctaag
    82381 cctgcggcac cgagtacagc aatgactgac gctgtttgta cgcccacgcg ccccagtgca
    82441 gcgattagcg taaaggcgat aataccgtaa cggactaatg cagagagaaa atcagcaaca
    82501 gtggcatcga ttttacggga gatcatcagg cgattcaccg cgttggaaat catccgcgcg
    82561 ataatcaaac caacaatgat gatcgcgagt gccgccacga tgtttactgc ataacttagc
    82621 agcagcgcct ggttagccac cagccagctt cccgcgccgt ttatgctatc gacaacattc
    82681 aaatcttcca ttcaatattc cttttcaact gactcaaaat ggagaaatac actccgccgc
    82741 cttatggcgg gcagtctgac aagggtaaac aataaagcat aattttgcca aatagatcac
    82801 agatttctca tctgcatcac cttgcttttg gcgaaaaaaa gacccgcaga gcgggccttg
    82861 agataagcag aaaggaatga cttacagaac gtcgatcgcg ttcagttcct ggaatgcttt
    82921 ttccagacga gcgatcatcg aagtctgacc ggcacgcagc catacgcgcg gatcgtagta
    82981 tttcttgttc ggctgatctt cgcctttcgg gttacccagc tgaccctgca aataagcttc
    83041 gttcgctttg tagtagttca gaacgccttc ccaggttgcc cattgggtat cggtatcgat
    83101 gttcattttc actacgccgt agcttacgga gtctttgatt tcctgagcag tagaaccgga
    83161 accaccgtgg aatacgaagt tcaggctgtt gtgcggcagg ttgtgtttct tagaaacata
    83221 ttcctgagag tcgcgcagga tggtcggagt cagaaccacg ttacccggct tgtaaacacc
    83281 gtgtacgtta ccgaaggacg ctgcgatggt gaaacgcggg ctgattttgc tcagctcggt
    83341 atacgcataa tcaacgtctt ccggctgggt gtacagtgca gaagcgtcca tgtggctgtt
    83401 gtccacgccg tcttcttcac caccggtgca acccagttcg atttccagag tcatgccgat
    83461 tttggacatg cgctccaggt atttagagca gatttcgatg ttctcttgca gagattcttc
    83521 agacaggtcg atcatgtgag aagagaacag cggcttgccg gtagctgcga agtgtttttc
    83581 acccgcgtcc agcagaccgt cgatccacgg cagcagtttc ttcgcgcagt ggtcggtgtg
    83641 caggataacc ggaacaccat aatgttcagc catctggtga acgtgatgcg caccagagat
    83701 cgcgcccagg atagcagcac cctgcggaac gtcagatttc acgcctttac cagcgataaa
    83761 ggaagcacca ccgttggaga actgaacgat aaccggcgct ttaactttag cagcggtttc
    83821 cagcacggcg ttgatggagt cagtaccgac gcagtttact gctggcagtg cgaagttgtt
    83881 ttcttttgct acctggaaaa ctttctgtac gtcatcacca gtgattacgc caggttttac
    83941 gaaatcaaaa atcttagaca tgtctcttgt cctgtatcgt cgggccttat actcgtcata
    84001 cttcaagttg catgtgctgc gtctgcgttc gctcacccca gtcacttact tatgtaagct
    84061 catgggattc actctcttgt cgccttcctg caactcgaat tatttagagt atgaaaaagt
    84121 gcgtgcttta aaagcgcgct gcaacaaggg caggtttccc tgccctgtga ttttttactt
    84181 cttagcgcgc tcttcgagca tcgctactgc aggcagtact ttaccttcca cgaattcgag
    84241 gaatgcaccg ccgccagtgg agatgtagga gattttgtca gcaatgccga acaggtcgat
    84301 tgctgccaga gtgtcgccgc cgccagcgat ggagaacgct tcgctgtctg cgatagcatt
    84361 agccacgatt tcagtacctt tgcggaagtt cgggaattcg aacacgccaa ccggaccgtt
    84421 ccacaaaatg gttttcgcat tcttcaggat ttcagccagt tcctgagcgg aagcatcacc
    84481 gatatccagg atctgctcgt cagctttcac atcgttaaca gatttcaggg tagccggtgc
    84541 agtttcagag aactcagttg ctacacgaac atcagacgga accgggatgt tgcaggtggt
    84601 cagcagacgt tttgcttcgt caaccagatc agcttcgtac agggatttac ccacatcgtg
    84661 gccttgtgcc gcgataaagg tgttagcgat accaccacca acaatcagct ggtcagcgat
    84721 tttagacagg gagtccagaa cggtcagttt ggtagatact ttagaaccac caacgatagc
    84781 caccatcggg cgagcaggtt ctttcagtgc tttacccagc gcgtccagtt cagctgccag
    84841 cagcgggcct gcgcacgcaa cgtcagcgaa tttaccgata ccgtgagtag aagcctgcgc
    84901 gcggtgagca gtaccgaatg cgtccattac gaacacgtca cacagtgcag cgtatttttt
    84961 ggacagggtt tcgtcgtctt tcttctcgcc tttgttgaag cgaacgtttt ccagaacaac
    85021 cagttcacct tcagcaacgt caacgccgtc gaggtaatct ttaaccagac gaaccgggtt
    85081 agacagtttg tctttcaggt agttaacaac cggcagcaga gagaattctt cgttgtactc
    85141 gccttcggta ggacgaccca ggtgggaagt taccatcact tttgcgcctt gtttcagggc
    85201 aagttcgatg gtcggcagag aagcacggat acgcgcgtcg ctggttactt tcccgtcttt
    85261 tactggtacg ttcagatccg cacggataaa tacacgtttc ccagcaagat ccagatcggt
    85321 catcttaatt acagacatgg tgaatcctct cgttgattct aaaagttttg cagacgctgc
    85381 ttgcgtctta cctgaaagca acagtagcca tagctaacgt cgtgtcgagc attcggttag
    85441 caaagcccca ttcgttatcg caccagacca acgttttgat caggtgtgcg ccactaaccc
    85501 gggtttgggt gccatcgaca atggcactgt gcggatcgtg gttaaaatct acagagacca
    85561 acggcaattc cgtatagtca actataccat gaaatgcacc ttgtgctgct ttttgcagca
    85621 acaggttgac ttcattggct tttacaggtt tcttcaccgt cacgcttaaa tcgattgccg
    85681 tcacatttat ggttggcaca cgtaccgcaa tcgcttcaaa gcgatcgtta aattgcggaa
    85741 aaaatcgtgt gataccggcg gccagtttag tatcgaccgg aatgatcgac tggctggcag
    85801 cccgggtgcg acgcaggtca ggatggtatg catcaataac ctgttgatcg tgcatggcgg
    85861 agtgaattgt ggtcacagtg ccggactcaa taccgtacgc atcatctaac aatttgatga
    85921 cgggaattat gcaattcgtg gtacaggaag cgttagaaac gatgcggtgt tccgcacgaa
    85981 gttgatcctg attgacgccg taaacaacgg tcgcgtcgag atcgttactg cctggatgtg
    86041 aaaagagcac ttttttggcc ccggcggcaa tatgcgcttc gccatgctcg cgggagccat
    86101 atacgccggt gcagtcgagg actacatcaa cgccaagttc acgccagggg agcgattgca
    86161 gtgaacgttc atgcaataca cggatggcgt catcaccaac aaaaagttga tcgcgttcct
    86221 gtcgtacttc ccatgcaaaa cggccatggc tggtgtcata tttcaacaaa tgcgccatgc
    86281 ccgcagcatc cgccagttcg ttgattgcca ccacggtaat ttccgcccgg cgtccggatt
    86341 catacaaagc acgaaccaca ttacgcccga tgcgaccgaa gccatttatc gctacgcgta
    86401 cggtcataga tctcctgcaa ggttttccct gagcaaattt gccagacaga gtaatccagc
    86461 aaatcgtccg gggaaacctt acctgtcgca aactgcgact gattggttaa ttgtcgaaca
    86521 tttaatcgac tgaaacgctt cagctaggat aagcgaaacg tggaataaaa ggaatgattg
    86581 tccagccgaa gaagacattt atctgactta catcacactt ttatcccctt ttgtaggggg
    86641 ctttattcca ggctggcgta ataatacccc tgcaataact ggaataaatt gtcagctttt
    86701 gaagtgatat ctgccttctt cggtcatctc taatatcaga ttggcctgac gactattcac
    86761 cagcacacgc tcgacattgg gaccatcggt tcgggcataa aaagcttcgg cttcttgccg
    86821 cgataatcct cccgcaattt tacgactaat caagcgctcc cgcagaattt gagcaggtgc
    86881 atgaataaaa atagaaaaat cgcagaaaga tgccaactcc tgccactttt catcatccag
    86941 taatagccag tttccttcga cgatgacgag tggtgcggta acgtgcaacg catcttcaac
    87001 aggatcatgc ttttgtcgat cgtactgcgg ccacgtacaa tccccttcca caacccggca
    87061 cagattttcc gccagtttcg caacgttgaa tgtctctggt gcgcctttga aggggcgcaa
    87121 ttgatgcgca tccagccagc tattgtaatg gtgaaaacca tccatcggca gtgtctgaat
    87181 agcgggcagt tccgggtctt gttgtgcgag atattcccag aaggtggtta gggtagattt
    87241 acctgtcccc ggcggagcgc aaagaaaaac caccgtacgc cgctgcggat ttaccgtctg
    87301 taaggccgcc aacatgtgca gcaacggttt atgtacattt tcaatctctt cgttctggta
    87361 ctgtgcctgt atcttcagcc cattcaccgt taattcaatt ttcacggcgt taattccttt
    87421 aaggtggtcg ttactgccag cgtcagcgcc gttttgacca tcctgctaaa acgcgcctct
    87481 gaccagacgg cgaaatcggc gaacttcatc gctttcaacg cgtcatagag cgtcgcattg
    87541 caggttatgc tattgacgtt gctgataaaa tcccaggtca tgtcatcggc aaatccgggc
    87601 gtttcatcct gttcgttcag gtaatgccaa aactgggaaa aatgcgtgat atcggcgtac
    87661 agccatttat ccattttccc cagcgcataa atcaaacgca gcgcaacatc aatatcgtca
    87721 agcgggccgc tttgggcgag taacggcttc actgcatatt cgacgatctc ttcatcgtta
    87781 tcgacaaaca gcgacggcag aaactggcgt atcccctgga gcaaaataga atgcgcagtt
    87841 ttcataaatg aaaataaatt gtcctgtgca tccagttgct caagcacatc atcttctgtc
    87901 agcgtcgcca ttggtcttcc gatagatcac catcaatgca gggagtctat tatattacac
    87961 gtttggtccg cgccgccgat cagtaatgtc tgcgtccgca ccccattcgc cgtctgttgg
    88021 ataccgttca ccaactcgcc gcccgtcacg cccgtagcgc taaagaggat atcgttcccc
    88081 ctcgccgata accactcgcc cacgaaaagc gacgtcgttt aatgcctggc gcatcgcggt
    88141 gaccgccagg ccatcaattt tatttttgtc gccacatccg gtttgcggcc aggcagccaa
    88201 cgctgcctgt tccgtaacgc gaaataatgg ccacgccagg gacatcattg cgcggcctcc
    88261 ccgatatcaa cgccaaattg cgccagcaga tatttctcgg cctgctcatt ccagatgccg
    88321 tgcgtttcct caaccagccg cgccagctct ttaaacagcg gatcggtttt gcctttttcg
    88381 gcaaaatcgg caatggcagt gagcggcatg gtgacgccgt tataaatgag ttttttaccg
    88441 ccggggatat ccggcagatt gagcacggta tcaggcaccg catccagccc accgatatgg
    88501 gtcaccataa acgacggctg caactgccct gtggcgctaa gcgcaatcgc ctctttcatg
    88561 tcgtccgttg aaccgccgga cgtaccgacg acatgcgtgc tgttgtaatg gacgttgtag
    88621 aaattaaacg gcactttgaa gtttttatcc gtcggcccgg caaagaagtt caggcagcca
    88681 tcttccgcca gtaattcatc agccatctcg acgactgaag gcaccgctgc atagacaaat
    88741 acgtcatcaa atccggcatc atccgtcagc gcccgcagcg tctggaccgg atcgctcatc
    88801 cctttggtat tcatatacac cagctcaatg cctttactgg ccgccagttc caccggcagc
    88861 agcttttgta cctgcgccag gcgtttgtcg tcgatatcga ccaccaccac ccgcgacggt
    88921 tgtatgccgc cgttaatagc ataatcgata gcgccaatgc ccatcggacc agcacatgcc
    88981 agcagcgcaa tattgccgcc aggtttaacg cccatgcgat gctcataaac atattgcgtg
    89041 gtgtgataat tggcatgata agcgccaata atgcagcaca taggctctgc cagcgacgcc
    89101 gcagcaaaat aagagccgtg atacggtaat acgcagccca ggttaatggc tatttcagga
    89161 ataatcatat aggtggcgtt accgccaaaa tattcgtagc tgtagcctgc tgaatatccg
    89221 cttggtaacc ccattgccgg ttgcaataca aaacgctggc cttttttata tttgtcggtg
    89281 agatttttac ccacttcgac aataaccccg gcgcattcat gcccggtaat gaccggatga
    89341 ttttctaaat cgtcgggtac gcgtttatgt tcactaccga gtagcgccgc tttccaggtg
    89401 gataaacaca cgctgtcaga aatcacactc accagcagtt cattatcggt aatttctggc
    89461 agttcaaatt cgcgcagacg gacatcccgc ttgccataaa tagcagcaac tttggttttc
    89521 atttttacct cggcaatcag tttttatgtt cggcggttaa ttgattaaat aaagtgtcga
    89581 gttttggatc gccaatatag ttattaatca gtatcagcgg tttatccgtc acacgtttca
    89641 cgcgcccttc cagactggca tgagtaacaa cgatatccgc atccgcaggc acgttttcta
    89701 tcgcgtaatg ttttacttct attgccagcc ccgccttttc caggcgttta cggaaggtgg
    89761 tcgcgcccat cgcgctggag cccatccccg catcgcaaac aaaggcgata cgcttaaccc
    89821 tgcttaacga gaatgcacct tcttgtttca tcgccttaac cgcattggct gactgagcaa
    89881 actcatcttc gctctccgtt tccaccgttt tttccatctt cagtattacg gaagtgatag
    89941 caaaggacac cagggtacct accgtaacac cggcaattgt cgccaggaac gagcctttcg
    90001 gcgtcagtgc caggtaagca aagatagacc ccggacttgg gccagccacc agaccaccgt
    90061 ccagtaagtt aaacatccag gtgccagaca taccgcccgc aatcatggca ataatggtca
    90121 gcggcttcat cagcacatac gggaaataca gttcgtggat cccaccgagg aagtgaataa
    90181 tcatcgcccc gggcgcagaa cgtttactca tccctttacc aaacaaagta aacgccagca
    90241 acagtcctaa gccaggccca gggttggacg ccaccataaa gaagatggat ttaccgttaa
    90301 ctgaagcctg ttgcatcccc aacgggtaat agacgccctg atctatcgcg ttattgagga
    90361 aaagcacttt cgccggttca ttgatgactg acagcaaagg cagataaccc gcatgtacca
    90421 acgcctcaat acactctttg acgaaagtat tggcaattaa caccgccggg ccgataactt
    90481 caaaacccag cagacaaagg agcatccccg caatacctaa tgagaagtta ttgataacca
    90541 tctcaaaacc agcagggatc cgcttttcca gcgccttgtc gatatattta atcaccagac
    90601 caccgagcgg ccccataatc attgagccaa ggaacatcgg gatctctgcg ccgacgatca
    90661 caccaatagt acctattccg cccatgaccg cgccgcgttt accgccgacc agatgaccac
    90721 cggtagaacc aatcatcacg ggcaataaat aggtaatcat cgggccgaca attttggcga
    90781 aatgttcgtt aggcagccag ccggtgggaa taaataacgc cgttataaaa ccccaggcta
    90841 taaaagcgcc gatattgggg atgaccattg cagtcaaaaa gcccccaaaa gcctggacct
    90901 ttgcacgagc agacttgttt tccataatat tatcctgtag aggagagagt aattatccgc
    90961 ggctgattat gtccgctaat tgtttttctg atgctgctgt taataattgg ttgagtattt
    91021 cttcttcaca caataattca cttaacgcct gaatagcgcc aatgtgcgaa tcggcatcgg
    91081 cagcagaaag cccgatgagt aattttatcg gttcgtcatt ccccggaaaa taaacgcctt
    91141 gtttaagtaa tgttaaagac atcccggttt taagcgcccc acattccggt cgagcatgag
    91201 gcattgccac gcctggtgcg agaatataat aagggccatt gttaatggtg gaatctttaa
    91261 ttgcctgaat gtaattctcg ctgatatagt ttttatccag caatgatgcc atcgagaaat
    91321 cgatagcttc ctgccaatct tttgctgaat gtataactga gattgatgat tctggaaaat
    91381 aatcaataag ccgcataaat aatccttatt ttatttttag ggcaccgtcc ggcggtaaaa
    91441 ataaaatact cgtgcggttt aatgaaagca attgggtacg cgagaagaaa aaccccatga
    91501 acaaaaataa taaaacttta aataacaaca acatgaaatt gatattttta aagtcaactt
    91561 tgtatttgtt atgtttatca caaaatcaac attttgattg ttgttgtatt tgtgatacat
    91621 atcgccattt atttattgaa tgtccgaaag catacttttc taatgcaatg ttttttcgct
    91681 ataccccttt atagattctg aattctcacc tgtcggctat tttctcctca cctcatgtga
    91741 catattcccc actttactca aggaagattc atatgaaagc acctgtttca ataacctcat
    91801 ttcgcccaca aaaatctatt gctatcgtca ttggcgtgct tgccgtggtt gtgctgccat
    91861 ttctctcgta ttacaccgtt aatgaaggtg aacgcggcat tttgctgcgc tacggaaaaa
    91921 tcgtaaaagt tgccgagccg ggtctgggtt ttaagatccc ttttatggaa agcgtggaga
    91981 aaatttccac gcgtaatcag gctgtggttt atcagggatt acaggcctac agccgcgacc
    92041 agcaaccggc acaaatgaca gtatcagtca gcttccacat taaaccttca gaagcaggcg
    92101 cggtttatac cacctacaac accattgaag cattaaaaga tcgtctgatt gtccgtcagt
    92161 tgccaactca actggagaac gttttcggcc aatataccgc catcagcgcc gtacaggatc
    92221 gtacaaaact ggttcaggat ctacaaaatg caatgcgtaa agccgttgtt ggcccggtgg
    92281 tgattgatgg tgtccagatt gaaaatattg atttttctga cgcttatgaa aaatcgattg
    92341 aagatcgaat gaaagcagaa gtcgccattg ccacacgtaa gcaaaatctg gaaacagaga
    92401 aaatccaggc gcaaatagcg gtaactcagg cccaggcaga agcagacagt aaactggcgg
    92461 cagccaaagc agaagctgag actattcgcg taagaggcgc ggcagaggct gaaactattc
    92521 gtttaaaaag tgcggctgaa gcagaagcga tccgcctgcg cggtgaagct ctgcgggata
    92581 atcccggact tgttgccctc accactgctg agcgttggga cggtaagcta cccgatacca
    92641 tgatccccgg cagtacagtt ccgtttatct ctactaagta aaaaaagggc cgctttcgcg
    92701 gcccttctca caaagcatct taccgattac agcagcgctt tcgctttcgc aacaacgttg
    92761 tcaacggtga aaccgaactc ttcaaacagt tgctctgcag gagcagattc accgaaggtg
    92821 gtcatgccga cgatagcacc gttcaggcca acatacttgt accagtagtc agcaataccc
    92881 gcttccaccg caacacgagc ggtaaccgct ttcggcagta cggattcacg gtaagccgca
    92941 tcctgcttgt cgaacgcgtc ggtagacggc atggaaacca cgcgcgcctt cacgccttcg
    93001 gcagtcagtt tttcgtaggc ggcaacagcc agttcaactt ccgaaccggt cgcgatgaag
    93061 atcagttccg gctgaccggc acagtctttc agcacataac caccgcgcgc gatgtttgcc
    93121 agctgctctt cagtacgctc ctgctgcgcc aggttctgac gggagaggat cagcgcggtc
    93181 gggccgtcct gacgctcaac gccgtatttc cacgcgaccg cggattcaac ctggtcacat
    93241 ggacgccatg tggacatgtt cggggtcacg cgcagagaag cgacctgctc taccggctgg
    93301 tgagtcgggc catcttcgcc cagaccgatg gagtcgtggg tgtaaaccat cacctgacgc
    93361 tgtttcatca gcgcagccat acgtacggcg ttacgtgcgt attccacgaa catcaggaag
    93421 gtggaggtgt acggcaggaa accaccgtgc aaggagatac cgttagcaat cgcggtcata
    93481 ccgaactcgc gaacaccgta gtggatgtag ttacccgcag catcttcgtt gattgcttta
    93541 gaaccagacc acagggtcag gttagacggt gccaggtcag cagagccgcc gaggaattcc
    93601 ggcaacagcg ggccgaaagc ttcgatagca ttctgagacg ctttacggct ggcgattttc
    93661 gccggattag cctgcagttt agcgatgaat tctttcgctt tggcatcgaa gtcagacggc
    93721 atttcgcctt tcatacggcg ggtaaattca gcggcttcct gcggataagc tttggcgtaa
    93781 gccgcgaatt tctcgttcca tgcggattct ttcgcctggc ctgcttcttt cgcatcccac
    93841 tgagcataga tttcagacgg gatttcgaac ggtgcatatt tccagcccag ctgttcgcgg
    93901 gtcagggcaa tttcagcgtc gcccagcggc gcaccgtggg agtcgtgcgt accggctttg
    93961 ttcggggaac cgaaaccgat gatggttttg cacatcagca gggacggttt gtcagtcact
    94021 gcgcgcgctt cttctactgc gcgtttgatg gatgccgcgt catgaccgtc gatgtcgcga
    94081 ataacgtgcc agccgtaagc ttcgaaacgc attgcggtgt cgtcggtaaa ccagccttca
    94141 acgtgaccat cgatagaaat accgttgtca tcgtagaacg caatcagttt acccagcttc
    94201 agcgtacccg ccagagagca aacttcgtgg gagatgcctt ccatcatgca gccgtcgccc
    94261 atgaaggcgt aggtgtagtg gtcgacgatg tcgtggcccg gacggttaaa ctgcgccgcc
    94321 agcgtttttt ctgcaatcgc cataccgact gcgttggcaa taccctgacc cagcggacct
    94381 gtagtggttt ccacgccagc ggtgtaaccc acttccggat ggcccggagt tttagagtgc
    94441 agctgacgga agtttttcag ttcttccatc ggcagatcgt aaccggtgag gtgcagcagg
    94501 ctgtagatca gcatggagcc gtggccgttg gacagcacga agcggtcacg gtcagcccag
    94561 gacggattct gcgggttgtg tttcaggaaa tcacgccaca ggacttcggc aatgtcagcc
    94621 atacccatcg gggcgcccgg gtgaccggat ttggctttct gtactgcgtc catgctcagc
    94681 gcacgaatag cattggcaag ctctttacgt gaggacattt tgactccaga tcggatgatg
    94741 aagggcacgc ccttaacgac ttgacgacag cgcgttttgg gctacgccgg aaaatttgcc
    94801 aacaatttac cgcaagccgc gcgtcatgta catggaacat ccttttgccg cttcagaaat
    94861 ctctggatca tgctcgcatg ttgcgcaatc tgctcgcccg tccgctgcgc ttttccttat
    94921 actgagactg agcgtcgatt cacctgcaaa cggcgcattt ttagaataat cctgaccttg
    94981 tgcggaagag aaaacatgaa aattcgcgcc ttattggtag caatgagcgt ggcaacggta
    95041 ctgaccggtt gccagaatat ggactccaac ggactgctct catcaggagc ggaagctttt
    95101 caggcttaca gtttgagtga tgcgcaggtg aaaaccctga gcgatcaggc atgtcaggag
    95161 atggacagca aggcgacgat tgcgccagcc aatagcgaat acgctaaacg tctgacaact
    95221 attgccaatg cgctaggcaa caatatcaac ggtcagccgg tgaattacaa agtgtatatg
    95281 gcgaaggatg tgaacgcctt tgcaatggct aacggctgta tccgcgtcta tagcgggctg
    95341 atggatatga tgacggataa cgaagtcgaa gcggtgatcg gtcacgaaat ggggcacgtg
    95401 gcgttaggcc atgtgaagaa agggatgcag gtggcacttg gtacaaatgc cgtgcgagta
    95461 gctgcggcct ctgcgggcgg gattgtcggc agtttatctc aatcacaact cggtgatctg
    95521 ggcgagaaat tagttaattc gcaattctcc cagcgccagg aagcagaagc cgatgattat
    95581 tcttacgatc ttctgcgcca acgcggcatc agcccggcag gtcttgccac cagttttgaa
    95641 aaactggcga agctggaaga aggccgtcaa agctcaatgt ttgacgacca tccagcatcc
    95701 gccgaacgcg cccagcatat tcgcgatcgt atgagcgcgg atggagttaa gtaagcattt
    95761 tattacgccg gatttgacgc ataacgcatc ttatccggcg cttactcgcc ctttttcgct
    95821 gcctgaatat acagcatctc cagcgccagc gtcgccgcag ccagagcggt gatttccgac
    95881 tgatcgtatg ccggagccac ttccactacg tccatcccaa caatgttgag atctttcagg
    95941 ccgcgtacca gtttaatagc gcgatcggag gtcaggccgc caattactgg cgtaccggtt
    96001 cccggcgcaa atgcaggatc caggcagtcg atatcaaagg tcaggtaaac cggcatatca
    96061 cccacaatct gtttcacttg ggcgataacg tcatccacgc tgcgatcgtt cacctggcag
    96121 gcgtccagca cggtaaagcc gttgtcttta tcgaactcag tacgaatacc aatctgcacg
    96181 gaatgattcg ggtcgatcag accttctttc ggcgcggtat agaacatggt gccgtggtca
    96241 aattcacaac cgttcgcata ggtgtcggtg tgggcgtcaa agtgtaccag cgccattttg
    96301 ccgaaatgct tcgcatgagc acgcagcagc ggcagcgtaa caaagtggtc accaccgaag
    96361 gagagcatac gcttaccggc agccagcagc ttctcggcgt gcgcctgcag cttctcgctc
    96421 atctcacggg catctccgaa ggcatatacc agatcgccgc agtccacgac gttcaggcgc
    96481 tcacgcatgt cgaaattcca cgggaagcga ttgtgttccc aggccagatt cgtcgaaacc
    96541 tgacggatcg ctgccggacc gtggcgacca cccgcacgac cagaagtggc catatcgaac
    96601 ggcacgccag taatcaccca gtcagcatcg ctgtcatatg gctggaagtt catcggcagg
    96661 cgtaaaaaac caaaggcgtt ggaaaccagt gagttatcgt attgatgacc taaggtgctc
    96721 atggatggac tcctattaca aagtcgatat aaaaaaaccc ttccgcgtcg ttaggcccga
    96781 cgaggaaggg ttggatttgc gaaacacaat tttgtgctaa ttatcgcctt aaatgggcga
    96841 ggcatcaagc gaggttgcta tttccgacac ggtggcggcg catagccatt gttatctgcc
    96901 ggaaaggctt gtccagagca cggcggcagc gcgttttgcg ccgggccttg taattcaaag
    96961 gcggcaaagc cgattacacc ggtattacgc acatcgcctt gcgccgaatt ggctgcgtag
    97021 gaatcagcaa cgttggcgaa ctgaaaagcc gcttcggtat gtttatcttt gcggaatcct
    97081 ttaatcgcta acgaatctcc ggcattcaca atatagccgt tgttattcag cgaaccttgc
    97141 ttgccgttca gcacatccag gccatcaacc gtagcaacga tctcgtagtt ggtattacga
    97201 ctgtaattac ggatatataa ttgataattc attccgactc tggcatggac aatatagcgt
    97261 ggttcattat tggatgaatc ataaacccgc gtaataggta gagaattaaa attaccatcg
    97321 cgaatagcat attccagttc gttaatacga atgctataaa cgcggtcata gccggaagga
    97381 acagtagtcg aataactaat aacgatcatc tctgccggat cgcgccagac tcgttcagca
    97441 gatacgccct gaaccgatga gtgtacatcc ccaccccagg cggtgccgac attgcctgat
    97501 gcactgctat tatactgcgc attactggaa caaccggcca gcagcacggc taatgctatc
    97561 aacgcgctac gtactttcca ttttttcata accgttccct ctgcatttaa ttaatgcagc
    97621 gattgtaggg ggattattta ataagaaaag cagctaaatt atttttaaga atatataaaa
    97681 agaaagcggg ccgatttacg taaataaatc gacccgccag cggaataatc ttaattattc
    97741 atcttcaaga taggtataac cgtacaaacc tgcctcgaac tcttcaagga actgttgttg
    97801 cagttcagca tcaagatcgg ttttcttcac ttgatcacgg aactgggtta acaacgtttt
    97861 cggatcgagc tgtacatatt gcagcatatc cgccacggta tcgccttcgt cagacagttc
    97921 tacttctacg ctgccgtcag ggaagacgaa cacgtcaacc gcttcggtat caccgaacag
    97981 gttgtgcatg ttgccgagga tctcctgata tgcgccgacc atgaagaaac cgagcatcgg
    98041 cggattctct ggatcgtact ccggcattgg cattgtcgtg gcaataccat caccatcgat
    98101 gtagtgatca atagcaccgt cagaatcaca ggtaatatcc agcagcacag cgcggcgctc
    98161 cggcacttga tccaaccctt ccagcggcag aaccgggaac aactggtcga tcccccatgc
    98221 gtccggcatc gactggaaca gcgagaagtt gacgtacatt ttgtcagcca tacgttcctg
    98281 cagctcgtcg ataatcggac gatgcgcacg gttttgcgga tccagttgct tctgcacttc
    98341 atggcacatg ctcagataga gttgttctgc ccatgcacgt tcttgcaggc taaaggtgcc
    98401 ggaagagtag ccgatatgaa tatcgtgcag atccatctgg ctgtcgtgta accattcacg
    98461 cagagaacga cgagttcccg gttcgtgcat ctcctgccag gtttcccaca tgctttgcag
    98521 ggcgcgcggt gcgtcttctg caggcgcggt cggcaccgtg tattcgttac gttccacgcc
    98581 gatgatattc gacaccagca cggtgtgatg cgcagtcacc gcacgacccg actcggtgat
    98641 taccgtcgga tgtggcagac cattttcttc acacgcatca ccaatcgccc agatgatgtt
    98701 gttggcgtat tcattgaggc cgtagttcac cgaacagtcg gactgcgaac gggtgccttc
    98761 atagtccacg cccagaccgc cgccaacgtc gaagcactga atattgacgc ccagcttgtg
    98821 cagttccaca tagaaacgcg cggattcacg aacgcctgtc gcgatatcgc gaatattcgc
    98881 catctgcgaa ccgaggtgga agtgcagcag ttgcaggctg tcgagacgcc cggcttcacg
    98941 cagcgtttca accagttgca gtacctgagt cgccgccagg ccgaacttcg atttttcccc
    99001 gccagaggac tgccatttac ccgagccctg cgaagccaga cgtgcacgca cgcccagacg
    99061 aggaacgaca ttcagacgtt ctgcttcatc cagcacaatg gcaatttctg acatcttctc
    99121 aatgaccaga tagaccttgt ggcccatctt ctcgccaatt aatgccaggc ggatatattc
    99181 gcggtcctta taaccgttgc agacgatgac gctacgggtc atgccagcat gtgccagcac
    99241 cgccatcaac tccgctttgg aaccggcttc caggcccagc ggttcgcccg aatgaatcag
    99301 ggactcaatc acgcggcggt gctggttaac tttgatcgga taaacgagga agtaatcgcc
    99361 gttatagccg taggattccc gcgcacgttt gaacgcggcg ttaatagaac gcaaacggtg
    99421 ctgcaggatc tgtgggaaac agaacagtgc aggcagacgc tggccctgtg cttcacgagt
    99481 tttcactaac tgcgcgagat cgacgcgagc ttccgggacg tccgggtccg ggcacacgct
    99541 aatgtggccc agctcgttaa cgtcatagta gttattgccc caccaggcaa tattgtaagt
    99601 acgcagcatc ttgctggctt cctgggagct cattgcaacc tcctgcatgg agcgtagtac
    99661 accgtgttcg cccgctgacg aaggcaaacc catagacatg tcgtcagaca tagcgaacct
    99721 caaattattt tattaagtgt aaaacagtta acgactatcg cagcctggaa ctgcgataac
    99781 aacccataag cacatggatt ttccagcagt gaatgctgac gctccaactg cgcaaccggt
    99841 ttctttttca taacattatt aagcacataa ccgaacgtaa gtgtgacagt tcggcgaaac
    99901 cacgagaaaa ctcttgtttt tacaagagcg cccttgttca gtcctcagta actgtaacca
    99961 gctcttgaat cctgagaagc gccgagatgg gtataacatc ggcaggtatg caaagcagag
   100021 atgcagagtg cggggaacga atcttcacca gaacggtgag acaggttaag cagcagacaa
   100081 cggttcatta tttcgtatca cctccacggc cgcctgttaa gacgaaccca caagccaaaa
   100141 ctctgatttc aacccggctg gaagtggcaa cacgaaagaa acgtcgtgtg ctttttattt
   100201 aagccgcgcg ccgcgtttta taccccacaa tggcagaaaa ttgcaaaaga taaatacgca
   100261 gaatgccagc attgtcagga aaaatttcca gccacgtttt taacagaatg agacacgatt
   100321 caaaaaaaag tggaaatcgg gtgaagaatt gacctaaaat agccatccag atgttaatcc
   100381 atccataccg attaacactc agactgccag tgtttttaac ctgcagagtc gtggtaggat
   100441 ccgctaccac agaaaatcca cacaacagtt tgagctaacc aaattctctt taggtgatat
   100501 taaatatggc aaaacacctt tttacgtccg agtccgtctc tgaagggcat cctgacaaaa
   100561 ttgctgacca aatttctgat gccgttttag acgcgatcct cgaacaggat ccgaaagcac
   100621 gcgttgcttg cgaaacctac gtaaaaaccg gcatggtttt agttggcggc gaaatcacca
   100681 ccagcgcctg ggtagacatc gaagagatca cccgtaacac cgttcgcgaa attggctatg
   100741 tgcattccga catgggcttt gacgctaact cctgtgcggt tctgagcgct atcggcaaac
   100801 agtctcctga catcaaccag ggcgttgacc gtgccgatcc gctggaacag ggcgcgggtg
   100861 accagggtct gatgtttggc tacgcaacca atgaaaccga cgtgctgatg ccagcgccta
   100921 tcacctatgc acaccgtctg gtacagcgtc aggctgaagt gcgtaaaaac ggcactctgc
   100981 cgtggctgcg cccggacgcg aaaagccagg tgactttcca gtatgacgac ggcaaaatcg
   101041 ttggtatcga tgctgtcgtg ctttccactc agcactctga agaaatcgac cagaaatcgc
   101101 tgcaagaagc ggtaatggaa gagatcatca agccgattct gccggctgaa tggctgactt
   101161 ctgccaccaa attcttcatc aacccgaccg gtcgttttgt tatcggtggc ccgatgggtg
   101221 actgcggtct gactggtcgt aaaattatcg ttgataccta cggcggcatg gcacgtcacg
   101281 gtggcggtgc attctccggt aaagatccat caaaagtgga ccgttccgca gcgtacgcag
   101341 cacgttatgt cgcgaaaaac atcgtggctg ctggcctggc cgatcgttgt gaaattcagg
   101401 tttcctacgc aatcggcgtg gctgaaccga cttccatcat ggtagaaact ttcggtactg
   101461 agaaagtgcc ttctgaacaa ctgactctgc tggtacgtga attcttcgac ctgcgcccat
   101521 acggtctgat tcagatgctg gatctgctgc acccgatcta caaagaaacc gcagcatacg
   101581 gtcactttgg tcgtgaacat ttcccgtggg aaaaaaccga caaagcgcag ctgctgcgtg
   101641 atgctgccgg tctgaagtaa tctttcttca cctgctttca aaggccagcc tcgcgctggc
   101701 ctttttcttt tacctcaccg cggtggacag gcattcacgc cgcatccggc aaaaaaaccg
   101761 cccgcacaat aacatcattc ctcctcaaca cgttttaccg caaattacca tcactactga
   101821 aaccgattac ac
//
LOCUS       DAZGQL010000003       100986 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.003, whole genome shotgun sequence.
ACCESSION   DAZGQL010000003 DAZGQL010000000
VERSION     DAZGQL010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 100986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 100986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..100986
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.003"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..689)
                     /locus_tag="ACHKIR_000202"
     CDS             complement(<1..689)
                     /locus_tag="ACHKIR_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE9 sensor domain-containing protein"
                     /protein_id="HFO9988010.1"
                     /translation="MLSLYEKIKIRLIILFLLAALSFIGLFFIINYQLVSERAVKRAD
                     SRFELIQKNVGYFFKDIERSALTLKDSLYLLKNTEEIQRAVILKMEMMPFLDSVGLVL
                     DDNKYYLFSRRANDKIVVYHQEQVNGPLVDESGRVIFADFNPSKRPWSMASDDSNNSW
                     NPAYNCFDRPGKKCISFTLRINGKDHDLLVVDKIHVDLNWRYLNEYLDHISANDEVLF
                     LKQGHEIIAKN"
     gene            complement(866..1777)
                     /gene="gsiD"
                     /locus_tag="ACHKIR_000203"
     CDS             complement(866..1777)
                     /gene="gsiD"
                     /locus_tag="ACHKIR_000203"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HFO9988011.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGASIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(1780..2700)
                     /gene="gsiC"
                     /locus_tag="ACHKIR_000204"
     CDS             complement(1780..2700)
                     /gene="gsiC"
                     /locus_tag="ACHKIR_000204"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HFO9988012.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVGDEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(2718..4256)
                     /gene="gsiB"
                     /locus_tag="ACHKIR_000205"
     CDS             complement(2718..4256)
                     /gene="gsiB"
                     /locus_tag="ACHKIR_000205"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0034634 - glutathione transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HFO9988013.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGLTYTVKLREGIKFQDGT
                     DFNAAAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQAALLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(4276..6147)
                     /gene="gsiA"
                     /locus_tag="ACHKIR_000206"
     CDS             complement(4276..6147)
                     /gene="gsiA"
                     /locus_tag="ACHKIR_000206"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HFO9988014.1"
                     /translation="MPHSDELDAGDVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDQMLLRRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGSVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     SGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKEAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYRGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVVAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(6134..7099)
                     /gene="iaaA"
                     /locus_tag="ACHKIR_000207"
     CDS             complement(6134..7099)
                     /gene="iaaA"
                     /locus_tag="ACHKIR_000207"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFO9988015.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLLAAREEG
                     AMVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            7303..8538
                     /gene="moeA"
                     /locus_tag="ACHKIR_000208"
     CDS             7303..8538
                     /gene="moeA"
                     /locus_tag="ACHKIR_000208"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HFO9988016.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VEVGEWVEVEPFNALFGGL"
     gene            8538..9287
                     /gene="moeB"
                     /locus_tag="ACHKIR_000209"
     CDS             8538..9287
                     /gene="moeB"
                     /locus_tag="ACHKIR_000209"
                     /EC_number="2.7.7.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HFO9988017.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAAMIAKHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYHCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCGVCGQ"
     gene            complement(9363..10025)
                     /gene="fsa"
                     /locus_tag="ACHKIR_000210"
     CDS             complement(9363..10025)
                     /gene="fsa"
                     /locus_tag="ACHKIR_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFO9988018.1"
                     /translation="MELYLDTSDVVAVKTLSRIFPLAGVTTNPSIIAAGKKPLEVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVDAAVAKFEQDWLGAFGRT
                     SI"
     gene            10156..11055
                     /gene="ybiY"
                     /locus_tag="ACHKIR_000211"
     CDS             10156..11055
                     /gene="ybiY"
                     /locus_tag="ACHKIR_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HFO9988019.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDPRLCLEGCELCAKTAPEVIERALNGLLIHREKLTPEHFTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVSEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            11061..13493
                     /gene="ybiW"
                     /locus_tag="ACHKIR_000212"
     CDS             11061..13493
                     /gene="ybiW"
                     /locus_tag="ACHKIR_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HFO9988020.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLTIAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIEMLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQAKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPIGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            13639..14454
                     /gene="ybiV"
                     /locus_tag="ACHKIR_000213"
     CDS             13639..14454
                     /gene="ybiV"
                     /locus_tag="ACHKIR_000213"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HFO9988021.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIEFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNIAPFNS"
     gene            14606..15865
                     /locus_tag="ACHKIR_000214"
     CDS             14606..15865
                     /locus_tag="ACHKIR_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001516052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HFO9988022.1"
                     /translation="MTFSCETLPTDAKAAIREMKAALREQLSDVQQVFDTLSAKIETR
                     VAEIDALKAQGLPVWPEVSYSDIAAGTVSDATRNEIKRRGCAVIKGHFPREQAMAWDR
                     AMLDYLDINHFDDVYKGPGDSFFGSLEASRPEIYPIYWSQAQMQARQSENMAAVQSFL
                     NRLWKSESNGKQWFNPDISVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQRV
                     FANVFNGNFDDYDPWDAAHRTDVEEYTVDNTTKCSVFRTFQGWTALSDMLSGQGLLHV
                     VPIPEAMAYVLLRPLLDDVPDDELCGVAPGKVLPISEKWHPLLLKALTSIPAITAGDS
                     VWWHCDVIHSVAPVDNQQGWGNVMYIPAAPMCEKNRAYAHKVKMALEKGASPGDFPRE
                     DYEANWEGRFTLEDLNIHGKRALGMDV"
     gene            complement(16106..17698)
                     /gene="ybiT"
                     /locus_tag="ACHKIR_000215"
     CDS             complement(16106..17698)
                     /gene="ybiT"
                     /locus_tag="ACHKIR_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HFO9988023.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIIGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            17917..18837
                     /gene="ldtB"
                     /locus_tag="ACHKIR_000216"
     CDS             17917..18837
                     /gene="ldtB"
                     /locus_tag="ACHKIR_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFO9988024.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(18895..20013)
                     /gene="ybiR"
                     /locus_tag="ACHKIR_000217"
     CDS             complement(18895..20013)
                     /gene="ybiR"
                     /locus_tag="ACHKIR_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HFO9988025.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGAQTPEWKPRLVWSCLGLYIVFLMALE
                     LKQELWGLVIVAVGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLSNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAW"
     gene            complement(20010..20477)
                     /gene="mntR"
                     /locus_tag="ACHKIR_000218"
     CDS             complement(20010..20477)
                     /gene="mntR"
                     /locus_tag="ACHKIR_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HFO9988026.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVYMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            20663..20791
                     /gene="mntS"
                     /locus_tag="ACHKIR_000219"
     CDS             20663..20791
                     /gene="mntS"
                     /locus_tag="ACHKIR_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HFO9988027.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            21063..22646
                     /gene="opgE"
                     /locus_tag="ACHKIR_000220"
     CDS             21063..22646
                     /gene="opgE"
                     /locus_tag="ACHKIR_000220"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFO9988028.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKESEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(22695..23210)
                     /gene="ompX"
                     /locus_tag="ACHKIR_000221"
     CDS             complement(22695..23210)
                     /gene="ompX"
                     /locus_tag="ACHKIR_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HFO9988029.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(23263..23328)
                     /locus_tag="ACHKIR_000222"
     CDS             complement(23263..23328)
                     /locus_tag="ACHKIR_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9988030.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            23563..24450
                     /gene="rhtA"
                     /locus_tag="ACHKIR_000223"
     CDS             23563..24450
                     /gene="rhtA"
                     /locus_tag="ACHKIR_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HFO9988031.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            24750..25253
                     /gene="dps"
                     /locus_tag="ACHKIR_000224"
                     /gene_synonym="pexB"
     CDS             24750..25253
                     /gene="dps"
                     /locus_tag="ACHKIR_000224"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HFO9988032.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            25657..26403
                     /gene="glnH"
                     /locus_tag="ACHKIR_000225"
     CDS             25657..26403
                     /gene="glnH"
                     /locus_tag="ACHKIR_000225"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015599 - ATPase-coupled L-glutamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1903803 - L-glutamine import across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HFO9988033.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            26542..27201
                     /gene="glnP"
                     /locus_tag="ACHKIR_000226"
     CDS             26542..27201
                     /gene="glnP"
                     /locus_tag="ACHKIR_000226"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HFO9988034.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            27198..27920
                     /gene="glnQ"
                     /locus_tag="ACHKIR_000227"
     CDS             27198..27920
                     /gene="glnQ"
                     /locus_tag="ACHKIR_000227"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HFO9988035.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            28037..30262
                     /gene="ybiO"
                     /locus_tag="ACHKIR_000228"
     CDS             28037..30262
                     /gene="ybiO"
                     /locus_tag="ACHKIR_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HFO9988036.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALTHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIHDETARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTIWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLVIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPAPGAAPAEPLPPGEPT
                     L"
     gene            complement(30259..31185)
                     /gene="rlmF"
                     /locus_tag="ACHKIR_000229"
     CDS             complement(30259..31185)
                     /gene="rlmF"
                     /locus_tag="ACHKIR_000229"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HFO9988037.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVAFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            31461..31721
                     /gene="mcbA"
                     /locus_tag="ACHKIR_000230"
     CDS             31461..31721
                     /gene="mcbA"
                     /locus_tag="ACHKIR_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein McbA"
                     /protein_id="HFO9988038.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTAIIYK"
     gene            31986..34268
                     /gene="fiu"
                     /locus_tag="ACHKIR_000231"
     CDS             31986..34268
                     /gene="fiu"
                     /locus_tag="ACHKIR_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HFO9988039.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQANADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGVG
                     AFFAGENGNSTTGDAIYMRGADTSNSIYIDGIRDIGSVSRDTFNTEQVEVIKGPSGTD
                     YGRSAPTGSINMISKQPRNDSGIDASASIGSAWFRRGTLDVNQVIGDTTAVRLNVMGE
                     KTHDAGRDKVKNERYGVAPSVAFGLGTANRLYLNYLHVTQHNTPDGGIPTIGLPGYSA
                     PSAGTAALNHSGKVDTHNFYGTDSDYDDSTTDTATMRFEHDINDNTTIRNTTRWSRVK
                     QDYLMTAIMGGASNITQPTSDVNSWTWSRTANTKDVSNKILTNQTNLTSTFYTGAIGH
                     DVSTGVEFTRETQTNYGVNPVTLPAVNIYHPDSSIHPGGLTRNGANANGQTDTFAIYA
                     FDTLQITRDFELNGGIRLDNYHTEYDSATACGGSGRGAITCPAGVAKGSPVTTVDTAK
                     SGNLVNWKAGALYHLTENGNVYINYAVSQQPPGGNNFALAQSGSGNSANRTDFKPQKA
                     NTSEIGTKWQVLDKRLLLTAALFRTDIKNEVEQNDDGTYSQYGKKRVEGYEISVAGNI
                     TPAWQVIGGYTQQKATIKNGKDVAQDGSSSLPYTPEHAFTLWSQYQATDDISVGAGAR
                     YIGSMHKGSDGAVGTPAFTEGYWVADAKLGYRVNRNLDFQLNVYNLFDTDYVASINKS
                     GYRYHPGEPRTFLLTANMHF"
     gene            34310..34987
                     /gene="ybiX"
                     /locus_tag="ACHKIR_000232"
     CDS             34310..34987
                     /gene="ybiX"
                     /locus_tag="ACHKIR_000232"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HFO9988040.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSRYGESEEILSLLNLYHNL
                     LREWSEI"
     gene            35061..35327
                     /gene="ybiI"
                     /locus_tag="ACHKIR_000233"
     CDS             35061..35327
                     /gene="ybiI"
                     /locus_tag="ACHKIR_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HFO9988041.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            35592..35852
                     /gene="ybiJ"
                     /locus_tag="ACHKIR_000234"
     CDS             35592..35852
                     /gene="ybiJ"
                     /locus_tag="ACHKIR_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YbiJ"
                     /protein_id="HFO9988042.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(35992..37077)
                     /gene="hcxB"
                     /locus_tag="ACHKIR_000235"
     CDS             complement(35992..37077)
                     /gene="hcxB"
                     /locus_tag="ACHKIR_000235"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HFO9988043.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAVVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGMPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQKTLQTSPDAILNCMTTII
                     INPELFGAPDCSAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARKVGMPEETLQAFCQQLAS"
     gene            complement(37218..38180)
                     /gene="ybiB"
                     /locus_tag="ACHKIR_000236"
     CDS             complement(37218..38180)
                     /gene="ybiB"
                     /locus_tag="ACHKIR_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HFO9988044.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPADKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDTAGSELLPQAKDPETTAQ
                     WIKRCLAGSEPIPESLKIQMACCLMATGEAATINDGLARINQAF"
     gene            complement(38208..40358)
                     /gene="dinG"
                     /locus_tag="ACHKIR_000237"
     CDS             complement(38208..40358)
                     /gene="dinG"
                     /locus_tag="ACHKIR_000237"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HFO9988045.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFREQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            40462..40662
                     /locus_tag="ACHKIR_000238"
                     /pseudo
     CDS             40462..40662
                     /locus_tag="ACHKIR_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADAR domain-containing protein"
     gene            complement(40897..42258)
                     /gene="rhlE"
                     /locus_tag="ACHKIR_000239"
     CDS             complement(40897..42258)
                     /gene="rhlE"
                     /locus_tag="ACHKIR_000239"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HFO9988046.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGRGQGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRKPA
                     AAQ"
     gene            42487..43158
                     /gene="cecR"
                     /locus_tag="ACHKIR_000240"
     CDS             42487..43158
                     /gene="cecR"
                     /locus_tag="ACHKIR_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HFO9988047.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     SDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            43161..44156
                     /gene="hlyD"
                     /locus_tag="ACHKIR_000241"
     CDS             43161..44156
                     /gene="hlyD"
                     /locus_tag="ACHKIR_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HFO9988048.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGNEAGHE"
     gene            44149..45885
                     /gene="ybhF"
                     /locus_tag="ACHKIR_000242"
     CDS             44149..45885
                     /gene="ybhF"
                     /locus_tag="ACHKIR_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFO9988049.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPTALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKETTPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            45878..47011
                     /gene="ybhS"
                     /locus_tag="ACHKIR_000243"
     CDS             45878..47011
                     /gene="ybhS"
                     /locus_tag="ACHKIR_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO9988050.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            47022..48128
                     /gene="ybhR"
                     /locus_tag="ACHKIR_000244"
     CDS             47022..48128
                     /gene="ybhR"
                     /locus_tag="ACHKIR_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO9988051.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(48090..48500)
                     /gene="ybhQ"
                     /locus_tag="ACHKIR_000245"
     CDS             complement(48090..48500)
                     /gene="ybhQ"
                     /locus_tag="ACHKIR_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HFO9988052.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            48633..49394
                     /gene="ybhP"
                     /locus_tag="ACHKIR_000246"
     CDS             48633..49394
                     /gene="ybhP"
                     /locus_tag="ACHKIR_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFO9988053.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKMQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            49391..50632
                     /gene="clsB"
                     /locus_tag="ACHKIR_000247"
     CDS             49391..50632
                     /gene="clsB"
                     /locus_tag="ACHKIR_000247"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HFO9988054.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAMFKAIGEAQERIILETFIWFE
                     DDVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            50632..51588
                     /gene="ybhN"
                     /locus_tag="ACHKIR_000248"
     CDS             50632..51588
                     /gene="ybhN"
                     /locus_tag="ACHKIR_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HFO9988055.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRIFSLSITTNWLGYILLAGIIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(51624..52013)
                     /locus_tag="ACHKIR_000249"
     CDS             complement(51624..52013)
                     /locus_tag="ACHKIR_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9988056.1"
                     /translation="MESYSQSSNKLDFQHEARILNGIWLITALGLVTTAGLALVIIRN
                     AALMSERPIWVISCLMIVLWSGIISHGRNKLLELAGKCHSEELWSPVSCAFTGALTLY
                     YYFIGFFGILAAIAITLVWQRHTRFFH"
     gene            complement(52219..52923)
                     /gene="ybhL"
                     /locus_tag="ACHKIR_000250"
     CDS             complement(52219..52923)
                     /gene="ybhL"
                     /locus_tag="ACHKIR_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFO9988057.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(53060..53512)
                     /gene="moaE"
                     /locus_tag="ACHKIR_000251"
     CDS             complement(53060..53512)
                     /gene="moaE"
                     /locus_tag="ACHKIR_000251"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HFO9988058.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            complement(53514..53759)
                     /gene="moaD"
                     /locus_tag="ACHKIR_000252"
     CDS             complement(53514..53759)
                     /gene="moaD"
                     /locus_tag="ACHKIR_000252"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HFO9988059.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADFPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSLDHPLTDGDEVAFFPPVTGG"
     gene            complement(53752..54237)
                     /gene="moaC"
                     /locus_tag="ACHKIR_000253"
     CDS             complement(53752..54237)
                     /gene="moaC"
                     /locus_tag="ACHKIR_000253"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HFO9988060.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(54240..54752)
                     /gene="moaB"
                     /locus_tag="ACHKIR_000254"
     CDS             complement(54240..54752)
                     /gene="moaB"
                     /locus_tag="ACHKIR_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HFO9988061.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(54774..55763)
                     /gene="moaA"
                     /locus_tag="ACHKIR_000255"
     CDS             complement(54774..55763)
                     /gene="moaA"
                     /locus_tag="ACHKIR_000255"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HFO9988062.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(55751..55895)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            56160..57068
                     /gene="yvcK"
                     /locus_tag="ACHKIR_000256"
     CDS             56160..57068
                     /gene="yvcK"
                     /locus_tag="ACHKIR_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFO9988063.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDAHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAISEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHSALEKALQALG"
     gene            complement(57260..59281)
                     /gene="uvrB"
                     /locus_tag="ACHKIR_000257"
     CDS             complement(57260..59281)
                     /gene="uvrB"
                     /locus_tag="ACHKIR_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFO9988064.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(59860..60537)
                     /gene="bioD"
                     /locus_tag="ACHKIR_000258"
     CDS             complement(59860..60537)
                     /gene="bioD"
                     /locus_tag="ACHKIR_000258"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFO9988065.1"
                     /translation="MSKCYFVTGTDTEVGKTVASCALLQAAKAAGYRTAGYKPVASGS
                     EKTPKGLRNSDALALQRNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAILHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALM"
     gene            complement(60530..61285)
                     /gene="bioC"
                     /locus_tag="ACHKIR_000259"
     CDS             complement(60530..61285)
                     /gene="bioC"
                     /locus_tag="ACHKIR_000259"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HFO9988066.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADALLAMLPQRKYT
                     HVLDAGCGPGWMSRRWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSMALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDKIEQSLNAVHYQHYIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            complement(61272..62426)
                     /gene="bioF"
                     /locus_tag="ACHKIR_000260"
     CDS             complement(61272..62426)
                     /gene="bioF"
                     /locus_tag="ACHKIR_000260"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HFO9988067.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWKQSAEQFGVGSGGSGHVSGYSVAHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSPLRRFAHNDVTHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLEEIQQVTQQHDGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSDTVADYLVQFARHLIYSTSMPPAQAQAL
                     RASLAVIRSDEGDTRREKLAALITRFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            complement(62423..63463)
                     /gene="bioB"
                     /locus_tag="ACHKIR_000261"
     CDS             complement(62423..63463)
                     /gene="bioB"
                     /locus_tag="ACHKIR_000261"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFO9988068.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLASARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            63550..64839
                     /gene="bioA"
                     /locus_tag="ACHKIR_000262"
     CDS             63550..64839
                     /gene="bioA"
                     /locus_tag="ACHKIR_000262"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HFO9988069.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKTQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRIYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEHADIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQH
                     QVAAIEGQLREQLAPACDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTVAVNRAVQDETFFCQ"
     gene            64898..65374
                     /gene="ybhB"
                     /locus_tag="ACHKIR_000263"
     CDS             64898..65374
                     /gene="ybhB"
                     /locus_tag="ACHKIR_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HFO9988070.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNIHFHSLASASITAMFS"
     gene            65526..66809
                     /gene="ybhC"
                     /locus_tag="ACHKIR_000264"
     CDS             65526..66809
                     /gene="ybhC"
                     /locus_tag="ACHKIR_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HFO9988071.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEVQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(66950..69211)
                     /gene="ybhJ"
                     /locus_tag="ACHKIR_000265"
     CDS             complement(66950..69211)
                     /gene="ybhJ"
                     /locus_tag="ACHKIR_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HFO9988072.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVAALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAEFTLSRRDPGYVGRSKATAELENQRLAGNVSELTEVFARIKQIAGQEHIDPL
                     QTEIGSMVYAVKPGDGSAREQAASCQRVIGGLANIAEEYATKRYRSNVINWGMLPLQM
                     AEAPTFEVGDYIYIPGIKAALDNPGTTFKGYVIHEDAPVTEITLYMESLTAEEREIIK
                     AGSLINFNKNRQM"
     gene            complement(69394..70827)
                     /gene="ybhI"
                     /locus_tag="ACHKIR_000266"
     CDS             complement(69394..70827)
                     /gene="ybhI"
                     /locus_tag="ACHKIR_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HFO9988073.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSGGEFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKRIAYLLIGKIGSTTLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            complement(70903..71955)
                     /gene="ybhH"
                     /locus_tag="ACHKIR_000267"
     CDS             complement(70903..71955)
                     /gene="ybhH"
                     /locus_tag="ACHKIR_000267"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HFO9988074.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLASIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISCSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            72139..73092
                     /gene="ybhD"
                     /locus_tag="ACHKIR_000268"
     CDS             72139..73092
                     /gene="ybhD"
                     /locus_tag="ACHKIR_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO9988075.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
     gene            complement(73133..74128)
                     /gene="pgl"
                     /locus_tag="ACHKIR_000269"
     CDS             complement(73133..74128)
                     /gene="pgl"
                     /locus_tag="ACHKIR_000269"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFO9988076.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSEDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            74283..75101
                     /gene="ybhA"
                     /locus_tag="ACHKIR_000270"
     CDS             74283..75101
                     /gene="ybhA"
                     /locus_tag="ACHKIR_000270"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HFO9988077.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIARGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(75102..76160)
                     /gene="modC"
                     /locus_tag="ACHKIR_000271"
     CDS             complement(75102..76160)
                     /gene="modC"
                     /locus_tag="ACHKIR_000271"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HFO9988078.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMA
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(76163..76852)
                     /gene="modB"
                     /locus_tag="ACHKIR_000272"
     CDS             complement(76163..76852)
                     /gene="modB"
                     /locus_tag="ACHKIR_000272"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HFO9988079.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(76852..77625)
                     /gene="modA"
                     /locus_tag="ACHKIR_000273"
     CDS             complement(76852..77625)
                     /gene="modA"
                     /locus_tag="ACHKIR_000273"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO9988080.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTASRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(77791..77940)
                     /gene="acrZ"
                     /locus_tag="ACHKIR_000274"
     CDS             complement(77791..77940)
                     /gene="acrZ"
                     /locus_tag="ACHKIR_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HFO9988081.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            78069..78857
                     /gene="modE"
                     /locus_tag="ACHKIR_000275"
     CDS             78069..78857
                     /gene="modE"
                     /locus_tag="ACHKIR_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HFO9988082.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARNHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            78925..80397
                     /gene="modF"
                     /locus_tag="ACHKIR_000276"
     CDS             78925..80397
                     /gene="modF"
                     /locus_tag="ACHKIR_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HFO9988083.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARAQAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAELLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGDFYRYALTKIN"
     gene            80658..81674
                     /gene="galE"
                     /locus_tag="ACHKIR_000277"
     CDS             80658..81674
                     /gene="galE"
                     /locus_tag="ACHKIR_000277"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFO9988084.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            81684..82730
                     /gene="galT"
                     /locus_tag="ACHKIR_000278"
     CDS             81684..82730
                     /gene="galT"
                     /locus_tag="ACHKIR_000278"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HFO9988085.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESHDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            82734..83882
                     /gene="galK"
                     /locus_tag="ACHKIR_000279"
     CDS             82734..83882
                     /gene="galK"
                     /locus_tag="ACHKIR_000279"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFO9988086.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLDATIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            83876..84916
                     /gene="galM"
                     /locus_tag="ACHKIR_000280"
     CDS             83876..84916
                     /gene="galM"
                     /locus_tag="ACHKIR_000280"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004034 - aldose 1-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HFO9988087.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPF
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNYLGGTPSRGTEPYTDWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAQ"
     gene            85119..85871
                     /gene="gpmA"
                     /locus_tag="ACHKIR_000281"
     CDS             85119..85871
                     /gene="gpmA"
                     /locus_tag="ACHKIR_000281"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFO9988088.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(86019..87071)
                     /gene="aroG"
                     /locus_tag="ACHKIR_000282"
     CDS             complement(86019..87071)
                     /gene="aroG"
                     /locus_tag="ACHKIR_000282"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HFO9988089.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLTLREELQDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLESGEPLAYGKSITDACIGWDDT
                     DALLRQLANAVKARRG"
     gene            87387..87767
                     /gene="ybgS"
                     /locus_tag="ACHKIR_000283"
     CDS             87387..87767
                     /gene="ybgS"
                     /locus_tag="ACHKIR_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HFO9988090.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQSNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHPDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            87881..88822
                     /gene="zitB"
                     /locus_tag="ACHKIR_000284"
     CDS             87881..88822
                     /gene="zitB"
                     /locus_tag="ACHKIR_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HFO9988091.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVIGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(88819..89538)
                     /gene="pnuC"
                     /locus_tag="ACHKIR_000285"
     CDS             complement(88819..89538)
                     /gene="pnuC"
                     /locus_tag="ACHKIR_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFO9988092.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQMFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVAMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(89576..90619)
                     /gene="nadA"
                     /locus_tag="ACHKIR_000286"
     CDS             complement(89576..90619)
                     /gene="nadA"
                     /locus_tag="ACHKIR_000286"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFO9988093.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCSVEEFNAFCDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGADILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVEMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPV
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            complement(90867..90942)
                     /locus_tag="ACHKIR_000287"
     tRNA            complement(90867..90942)
                     /locus_tag="ACHKIR_000287"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(90907..90909),aa:Lys,seq:ttt)
     gene            complement(90976..91051)
                     /locus_tag="ACHKIR_000288"
     tRNA            complement(90976..91051)
                     /locus_tag="ACHKIR_000288"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(91016..91018),aa:Lys,seq:ttt)
     gene            complement(91198..91273)
                     /locus_tag="ACHKIR_000289"
     tRNA            complement(91198..91273)
                     /locus_tag="ACHKIR_000289"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(91238..91240),aa:Lys,seq:ttt)
     gene            complement(91277..91352)
                     /locus_tag="ACHKIR_000290"
     tRNA            complement(91277..91352)
                     /locus_tag="ACHKIR_000290"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(91317..91319),aa:Val,seq:tac)
     gene            complement(91404..91479)
                     /locus_tag="ACHKIR_000291"
     tRNA            complement(91404..91479)
                     /locus_tag="ACHKIR_000291"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(91444..91446),aa:Lys,seq:ttt)
     gene            complement(91482..91557)
                     /locus_tag="ACHKIR_000292"
     tRNA            complement(91482..91557)
                     /locus_tag="ACHKIR_000292"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(91522..91524),aa:Val,seq:tac)
     gene            complement(91693..91768)
                     /locus_tag="ACHKIR_000293"
     tRNA            complement(91693..91768)
                     /locus_tag="ACHKIR_000293"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(91733..91735),aa:Lys,seq:ttt)
     gene            complement(91933..92724)
                     /gene="cpoB"
                     /locus_tag="ACHKIR_000294"
     CDS             complement(91933..92724)
                     /gene="cpoB"
                     /locus_tag="ACHKIR_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0070206 - protein trimerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HFO9988094.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSGDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     EAIVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(92734..93255)
                     /gene="pal"
                     /locus_tag="ACHKIR_000295"
     CDS             complement(92734..93255)
                     /gene="pal"
                     /locus_tag="ACHKIR_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HFO9988095.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(93290..94582)
                     /gene="tolB"
                     /locus_tag="ACHKIR_000296"
     CDS             complement(93290..94582)
                     /gene="tolB"
                     /locus_tag="ACHKIR_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HFO9988096.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELCVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(94715..95980)
                     /gene="tolA"
                     /locus_tag="ACHKIR_000297"
     CDS             complement(94715..95980)
                     /gene="tolA"
                     /locus_tag="ACHKIR_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HFO9988097.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAAEAQKKAEAAAAALKKKAEAAEAAAAEARKKAATEAAEK
                     AKAEAEKKAAAEKAAADKKAAAEKAAADKKAAEKAAAEKAAADKKAAAEKAAADKKAA
                     AAKAAAEKAAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASG
                     ADINNYAGQIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAAL
                     AAAKLAKIPKPPSQAVYEVFKNAPLDFKP"
     gene            complement(96045..96473)
                     /gene="tolR"
                     /locus_tag="ACHKIR_000298"
     CDS             complement(96045..96473)
                     /gene="tolR"
                     /locus_tag="ACHKIR_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HFO9988098.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(96477..97169)
                     /gene="tolQ"
                     /locus_tag="ACHKIR_000299"
     CDS             complement(96477..97169)
                     /gene="tolQ"
                     /locus_tag="ACHKIR_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HFO9988099.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(97166..97570)
                     /gene="ybgC"
                     /locus_tag="ACHKIR_000300"
     CDS             complement(97166..97570)
                     /gene="ybgC"
                     /locus_tag="ACHKIR_000300"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFO9988100.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(97720..98013)
                     /gene="ybgE"
                     /locus_tag="ACHKIR_000301"
     CDS             complement(97720..98013)
                     /gene="ybgE"
                     /locus_tag="ACHKIR_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HFO9988101.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(98013..98126)
                     /gene="cydX"
                     /locus_tag="ACHKIR_000302"
     CDS             complement(98013..98126)
                     /gene="cydX"
                     /locus_tag="ACHKIR_000302"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HFO9988102.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(98141..99280)
                     /gene="cydB"
                     /locus_tag="ACHKIR_000303"
     CDS             complement(98141..99280)
                     /gene="cydB"
                     /locus_tag="ACHKIR_000303"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFO9988103.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFHLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(99296..100864)
                     /gene="cydA"
                     /locus_tag="ACHKIR_000304"
     CDS             complement(99296..100864)
                     /gene="cydA"
                     /locus_tag="ACHKIR_000304"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HFO9988104.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
BASE COUNT        24511 a        27000 c        25326 g        24149 t
ORIGIN      
        1 tgattcttgg caatgatctc atgaccttgc tttaaaaata gaacttcatc attagcgctg
       61 atgtgatcaa gatactcgtt cagatatcgc cagtttaaat cgacatgaat tttatccacc
      121 actaacaaat cgtgatcttt gccgttgatg cgtagcgtaa aagagataca ttttttaccc
      181 ggacgatcaa agcaattgta tgccggattc cagctgttgt tagagtcatc tgaagccatc
      241 gaccacggtc gtttcgatgg gttaaaatcg gcaaaaataa cccgccctga ctcgtcgaca
      301 agcggtccat ttacttgttc ctgatgataa acaacgattt tatcattcgc cctccgcgaa
      361 aagagataat atttattatc atcaagtacc agtcccaccg agtctaaaaa tggcatcatt
      421 tccattttca gaattacggc gcgttgaatc tcctctgtat tttttaataa atatagtgag
      481 tcctttaatg tcagggccga acgttcaata tctttaaaga aatagccaac gtttttctga
      541 ataagttcaa agcggctatc ggcacgtttt accgcgcgtt ccgataccag ttgatagtta
      601 atgatgaaaa aaagaccaat aaatgacagt gctgccaata aaaataaaat tatcagcctt
      661 atctttatct tttcgtataa actcagcatg ttaatacatt taacaaaata actgtctgaa
      721 cgctgtgccg ctgcaaaata gtcccttgct gcggcttttt aaccaaatta cccgattcta
      781 caaaatcagc agaaagttac aacacttgct tagggcaata atttcctgtg aggtgattac
      841 cctttcaagc aatattcaaa cgtaactatc ctttaatttt cggatccagc gcatcgcgta
      901 aaccatcgcc caacaaattg aacgccagta cggtcagaaa aatagccagg gccggaaaaa
      961 cagcgacatg cggcgcgata accatatccg ctcgtgcctc attgagcatt gcaccccact
     1021 ctggtgtcgg cggctgcgca ccgaggccga gaaatgacag gctggcggca gagataatcg
     1081 aggcgccaat gcgcatggtg aaaaacacca cgatagaaga gacggtccca ggcaagatat
     1141 gacgcaacaa aatggtcata tcgctggcac caatactgcg tgctgactcg ataaaggttt
     1201 gctgtttcaa caccagcgtg ttgccgcgca ccagccgggc aaacgcgggg atggaaaaaa
     1261 tggcgactgc gataatcacg ttagcaatgc cgcttcccaa caccgcaaca acagcgatcg
     1321 ccagtaaaat acccgggaag gcaaacaaca catcgcaaat gcgcatgatc agccgatccc
     1381 accagccttc ataatatcca gccagcaagc ccagcaacgt cccgatcgcc gcaccgataa
     1441 acacggcaaa cacgcccgcc gccagcgata tttgcgcacc aaccaggaca cggctgaaaa
     1501 tgtcgcgccc cagtgaatcg acaccaaacc agtgctgcaa agaaggtccg ttattcagat
     1561 tgtcataatc aaaataattt tcggcgtcat agggagcgat ccagcgtgca aaaatggcca
     1621 ccacaatcaa taaaataacg aacaatgcgg cggtcatcgc catatgctga cggcgaaatc
     1681 gtcgccagaa ttcatgccac ggtgtacgta cctggtcagg tttgaccagt ggcatggcgt
     1741 ttaacaccgc ctgacgtcgc cagttaaata gtcgcatcct tacttgtacc tgatagccgg
     1801 gttaatggcg gcgtaaagca catccaccac taagttgata agaataaatt ccagcgagaa
     1861 aagcagaatt tccgcctgaa tcaccgggta atcgcgcatt tcaacggagt caacgagtaa
     1921 gcgtccaagt ccgggccagt tgaaaacttt ctccaccacg atggaaccac cgagtaaaaa
     1981 tccaaactgt aagcccatca tggtcactac cgggatcatc gcgttgcgta gcccgtgttt
     2041 gaggacaacc caggtttcgc tcaccccttt cgccctcgcg gtacgcatat aatcttcgct
     2101 taaaacgtcg acaaacgacg cgcgggtaaa gcgcgccatc acggcggcca ctgccgcgcc
     2161 gagcgtcagg gagggtaaaa tgtagtgctg ccaactgtct gctcccacgg taggcagcca
     2221 gcccagttca acagagaata cctgaattaa aagcatcccc agagcaaatg ccggaaacga
     2281 aatccccgac accgcaatag tcatactcaa tcgatccggc caacggttac gccagacggc
     2341 ggcgataatt cccgccgcca taccaaatat caccgcccag accatactgg ttatggtcag
     2401 ccacagcgtt ggcataaagc ggctggcaat ctcatcgcca accggacgac gcgacaccat
     2461 cgacaggcca aaatccccct gcacagcatt gctgatatag tgccagaact ggtgatacag
     2521 cggctgatcc aaccccagct gctgacgcac cagttctata acctgcgcat cagcttcggg
     2581 cccggcaatt aatcgcgccg gatcgccggg cagcatgtgg acaaataaaa acaccagcac
     2641 cgagacgata aacagcgtcg gaatcaaccc cagtaagcgt ttgataacgt aattaagcat
     2701 tccactccct gcgttgctta ttgcaaatcc gcatcttcaa agctgaagcc ggtgtctggc
     2761 atgatccaaa aaccggtcag gtttttactg tgtgccgaca caagtttttc taccaccagc
     2821 gggatccacg gcgactcttg ccagatgata tcctgcgccg ccttatacaa gcgggtcttt
     2881 tccgccggat cattagtttt cagtgcctga gccaggaagt catccacctg tttattgctg
     2941 taaaacgcgg tattaaacag cgttggtggc cagttctgcg aggcaaacag cggcgataga
     3001 gcccagtcgg cttcgccggt tgaagccgac cagccagtgt agaacatccg cacgccgctc
     3061 tctttttgcc ctttaccttc aacttctgcc gcccgctgtc cggcatccat cgcagtcacc
     3121 tgggctttaa tcccaacctg cgctaactgc tgctgggtaa attgcagcac tttctgcgcg
     3181 gtgctgtggt tatgtgacga ccacagcgtg gtactgaaac cgttgggata tcccgcctct
     3241 ttcagtaatt cgcgcgcttt cactggatcg taaggccacg gtttataact ttgcgcgtag
     3301 gcgatactcg gcggtaccac accagtagct ggcgttgcat agcccgcgaa ggccactttc
     3361 accaacgccg ggcggttaat ggcgtaattc agcgcctcac ggaccttcgg gttatcgaac
     3421 ggcttttgcg tcacgttcat actgatataa cgctgcataa ttgacggact ggccatcaac
     3481 tcgatatttt tgtttttctc cagcagtgcg gcctgctcgt agggaatggg gaaagcaaac
     3541 tgcgcttcac cggtttgcag cattgccgcg cgggtgttgt tatccgccac cggacgccag
     3601 gttatgctgt ccagtttggg caatcctggc tgccagtaac ccgcgaattt tttcaccttc
     3661 acaaaatcgg tctgattcca ggtatccagt tcatacggcc cggttcccac cggatgaaaa
     3721 ccaatctcct tgccatattt ttccagcgct gccggtgaaa tcatcgcggt cgccggatgg
     3781 gcaagaatat taataaacgc tgagaatggc tgtttgaggg taatctttac cgttgtcgga
     3841 tcgatcgctt ccgttttggc aatattctta tacaggttat agcgtttaag atgattcgcc
     3901 ggatcgctgg cccggtccag attcgctttt accgccgctg cgttgaagtc ggttccatcc
     3961 tggaatttaa tgccttcccg cagtttcacg gtgtaagtaa ggccgtcatc ggaaacggta
     4021 taactctccg ccagcacgtt tttcagtttc atctctttat ccagaccgaa cagcccctgg
     4081 taaaacgatt tcgctacggc ctgagataac gtgtcattcg cgtcatatgg atcgagcgtg
     4141 gtgaagttcg atcctaccgc caccaccaca tctttggcag cgaatgcaca agatgccatc
     4201 aacgctgtcg caatgcccag cgccactaac ccactacggt gtacagctct tgccatttta
     4261 ttctccgcct gaatgttatc tacgcatgaa tgcgtattct gattgtggtt gtgcgacgta
     4321 atgccccggc ccgacgcatt gcaacgagac ggctacaacc tcttcaccac gcagatgaat
     4381 attgctggga agatcgtccg acagcagtac acgctgcggt cgttgtcggg acggttcagc
     4441 gaccggaact gccgccagta atttacgcgt ataaggatgc tgcgggtttt cgaagaccgc
     4501 gcgccgtgga ccaatttcaa caatttgtcc gcgatacatc accgccacac gatgactaat
     4561 ccgctctacc acggccatat cgtgggagat aaacagatac gcaatgccga aatcacgctg
     4621 gagatcgagc aacaagttga taatctgccc gcgaatagaa acatccagcg ccgagacggc
     4681 ttcgtcggca atgatcactt ttggattcaa tgccaacgcg cgagcaatgc agatgcgctg
     4741 gcgctgaccg ccggaaaact catgagggta acgccaggca tgttccggta acaggcccac
     4801 gcgctccagc aaccacgcaa cgcgtgcagc cgcttcttta cctggcaata aaccgtgtac
     4861 gcgcagcggt tcgataatcg aatcaccgat ggtctgacgt gggtccagcg aagcgtaagg
     4921 gtcctgaaaa ataaactgaa tatcccggcg caaagcctga agtttaccgg atgacaaggt
     4981 atcgattcgc tgaccgttaa agataatttc gccgccctgc gattcgacca ggcgcagcaa
     5041 cgcccgcccg gtagtggatt taccgctgcc agactcgccc accagcgata gcgtttcgcc
     5101 aggccagaga tcaaaactga ctttctcaac ggcatgcact tcccgcgtta cgcgattcaa
     5161 caaaccgctg cgcaaaggga aacgggtgac cagattacgc acccgtaaaa caggttcgcc
     5221 atccaccacc gttttctgct cgatgggggg ttcctgtttt gctggatgtt caagcgatat
     5281 caatgggaaa cgtcggggat aatctaaccc tttcatcgca ccaagttgcg gaacagcagc
     5341 taacagcgca cgggtataag gatgttgcgg tgcatgaaaa atctgttcga cgctacccgt
     5401 ttccaccgcc tcgccctgat acatcaccag tacccgatcg gcaatctctg ccaccacgcc
     5461 catatcgtga gtgataaaga taacgcccat cgacatctct ttttgcaata ctttgattaa
     5521 ttgcaggatc tgcgcctgaa tagtgacatc caacgcggtg gttggctcat cggcaatcag
     5581 caccgccggg cggcatgaca gcgccatcgc aatcatcact cgctggcgca tcccgccaga
     5641 gagttgatgc ggataacgtg aaaggatggt ttgtgcctcc ggaatgcgta cctgatccag
     5701 catccgcttc gcctcgacca tcgcttcttc acgactggcg ttctgatgca gacgaattga
     5761 ttcggcaatc tgttcaccca cagtaaatac cgggttcagc gatgtcatcg gttcctgaaa
     5821 tatcatcgcc atatccgcac cgcgcacatg gcgcatttgt gcagcgctct gctcgctaag
     5881 ttcaatcact tcacgactgc gccgccgcaa cagcatttga tcgcactgca ctaaaccgcc
     5941 cgcctgttcc aacaagcgca tcaatgccag cgcagtcact gacttaccag agccggattc
     6001 gccaacaatt gccagcgtct caccgcgttg caggctaaaa gagagattgc ggaccgcagc
     6061 tattttctgc tggtcctgca taaaggcaat attcagattt tcaaccgcca gcacatcacc
     6121 ggcatcaagt tcatcactgt gtggcaacgg tgtccccttt ttcacggtag ataccggtgg
     6181 ttggcgtatc gcctgcgtag ccccaggcgc gatacattcc ttcggtgtta aacggtagcg
     6241 cgacgttacc ttcatggtcg atagcgatta agccaccgct accgccaagc gcaggaagtt
     6301 tttccattac tacccgctcg caggcttccg cgagacttaa tccgccgtaa tccattaacg
     6361 cggcgatgtc atatgccgcc agcgcgcgga tgaagacttc gcccgtgccg gtacaagaaa
     6421 ccgccacact ggcgttattg gcgtagcatc cggcacccac taaggggcta tcgccaactc
     6481 gtccgggtaa tttattggtc attccgcccg tggacgtagc tgccgccaga ttgccgtcta
     6541 aatccaacgc cacggccccc acggtgccca ttttctgttt ttcatccagt ggcgcaccgc
     6601 tatggtcgag aaccattgcc ccttcctcgc gcgctgccag tagttgttca taacgcaaag
     6661 gcgtggagaa aatctccggc gagacgcgct ccatgccatg agcaaacgca aaattttctg
     6721 ccccttcgcc aatcatcatc acatgcgggc tttgctccat caccagccgg gcggcaagaa
     6781 ccggattacg cagatgacta acgcctgcca ccgcaccggc tttcagggtg ttaccatcca
     6841 tcacacaggc gtccagttca tgggtttcat cacgcgtaaa gacagcgcca attccggcgt
     6901 taaacagtgg acactcttcc agcagacgca ccgcttccgt caccacatcc agcgcacttt
     6961 cgcccgcttc cagcattttc tgcccggttt caacaatggc agacagcgcc tcgatgtagc
     7021 gtaattcctg ttgcagactc atctgcgcgc ggctaattgc acctgcgccg ccatgaattg
     7081 caatgactgc tttgcccata gtattcgtcc attaagggtg gctcgccact tttgctataa
     7141 atatcagaat ttttatgaat tactcattca atttttgaat atagaaagat ggatatggca
     7201 tgtaaaggca ggagccaacg ctacgcccga ggtggcatac agtctgtggc gtttttctga
     7261 cataataggc aaattcgatt ttgcctccgc aggagtgttt tcatggaatt taccaccgga
     7321 ttgatgtcgc tcgacaccgc gcttaatgag atgctttctc gcgttacccc actgaccgcc
     7381 caggaaacgc tgccactggt tcagtgtttt ggtcgtattc tggcgagcga tgtcgtttcg
     7441 ccactggatg tcccggggtt tgataactcc gctatggacg gctacgcggt gcgtttagcc
     7501 gatattgcct ccgggcaacc gctgcccgtt gccggtaaat cctttgccgg tcagccgtac
     7561 catggtgaat ggcctgcggg tacctgcatt cgtattatga ccggtgcgcc ggtgccagaa
     7621 ggctgcgaag cggtggtgat gcaggagcag actgaacaaa cggacaatgg cgtgcgtttt
     7681 actgctgaag tgcgtagcgg gcaaaatatt cgccgtcgcg gtgaagatat ctctgcaggt
     7741 gcggttgttt tcccggcggg gactcgcctg actaccgctg aactgccagt gattgcttcg
     7801 ctagggattg ccgaagttcc ggtgattcgt aaagtgcgtg tagcgctttt ttctaccggt
     7861 gatgaactcc agttgcccgg tcagccgctg ggcgacggcc aaatctacga taccaaccgt
     7921 ctcgccgtac acctgatgtt agaacagttg ggatgcgagg taatcaactt agggattatc
     7981 cgcgacgatc cccatgccct gcgcgccgca tttattgaag ccgacagcca ggcggatgta
     8041 gtgatcagtt ccggcggtgt ttcagtgggt gaagcggatt acaccaaaac gattcttgaa
     8101 gagctggggg agatcgcctt ctggaaactg gcgattaaac cgggtaaacc gttcgcgttc
     8161 ggtaaacttt ctaatagttg gttctgcggc ctgccgggca acccggtttc agcgacgctg
     8221 accttctatc aactggtaca gcctttgctg gcaaaactaa gcggcaatac cgccagtggc
     8281 ctgcccgcgc gccagcgtgt acgcacagcg tcccgcctga aaaaaacgcc aggacgcctt
     8341 gatttccagc gcggcgtgct gcaacgcaac gccgatggcg aactggaagt gacgaccacc
     8401 ggacatcagg gttcacatat atttagctcc tttagcctcg gcaactgctt tatcgtgctg
     8461 gaacgcgatc gcggcaatgt ggaagtgggc gaatgggtgg aagtagaacc gtttaacgcg
     8521 ttgttcggag gcctgtaatg gcggaactca gcgatcagga gatgctgcgc tacaaccggc
     8581 aaatcattct gcgcggtttt gattttgacg gtcaggaagc attgaaagat tcccgcgtgc
     8641 tggtcgtcgg ccttggtggg ctcggctgcg cagcttcgca gtatctggca agcgccggtg
     8701 tcggcaacct gacgctgctc gacttcgaca cggtttcgct ctccaatctg caacgccaga
     8761 cgctgcacag tgatgccacg gtcgggcaac caaaggtgga atccgcccgt gacgccctga
     8821 cgcggatcaa cccacatatc gcgattacgc cagtcaatgc gctgcttgat gacgctgaac
     8881 ttgctgcgat gattgcaaaa cacgatctgg tgctcgactg tacggataac gttgcagtac
     8941 gtaatcaact gaacgcaggc tgttttgccg cgaaggtgcc gctggtttcc ggcgcggcaa
     9001 ttcgtatgga agggcaaatc acggtcttca cttatcagga cggcgaaccg tgctatcact
     9061 gccttagccg tttgtttggc gagaacgcgt taacctgcgt agaagcaggc gtgatggcac
     9121 cgttaatcgg cgtaattggt tcgttgcagg caatggaagc aatcaaactg ctggcaggtt
     9181 atggcaaacc agccagcgga aaaatcgtga tgtacgatgc gatgacctgt cagtttcgcg
     9241 aaatgaaact gatgcgcaat ccggggtgtg gggtgtgcgg gcagtaactg ccacctccag
     9301 gccagataag acgcggcact ccgccacgtc atcagcaatt tatgaggatg cagaacgtcc
     9361 ggttaaatcg acgttctgcc aaacgctccc agccagtcct gttcaaactt cgccacagcg
     9421 gcatcaaccg ccggatagct aatcatctgt tgtgccacat ccagcggcag agtaattgat
     9481 tcacatcctg ccagtaagca gtccagcgcc tgacgcgggg ttttgaaact cgcggctaac
     9541 actttcgcct gcggcgcatg cattttcaat aactggtgta agtcggtcac agtctgaata
     9601 ccgctaccgc cctgagcatc aatacgatta acgtaaggcg caacatattc cgcacctgcc
     9661 agcgccgaca gtagcccttg tgctgcgcca tataccgcgg ttcccagcgt cggaatccct
     9721 tccgctttta acatcttaat agctgccagc ccctcagcgg tcaccggaac cttcaccacg
     9781 atatccgcaa taatagaacg caatttacgt gcgtcattaa ccatcccttc agcagtggta
     9841 gccattacct gggcaaacag acgcccctga ccgcccatcg cctcgtgaag ttgcggaagc
     9901 acaacctcca gcggtttttt acccgctgcg ataatgcttg ggttagtggt cacacccgcc
     9961 agcggaaaaa tacgtgacag cgtcttcacc gcaacaacgt ctgaagtatc cagatacagt
    10021 tccataacca tcctcaaaat gtactttttg tctgaatgta gactatcacg ggaaaactct
    10081 ctgaacagtt gacgcacatc aatctaactt tcattcgaaa gtattttaat gtttgaacga
    10141 aacagagagg caactatgat tttcaatatt caacgctact cgacccatga tggccccggt
    10201 atccgcacgg tcgtatttct taaaggctgt tcgctgggct gccgctggtg tcagaacccg
    10261 gaaagccgcg cccgcacgca ggatctgctg tatgacccac gactctgtct ggaaggctgt
    10321 gagctgtgcg ctaagaccgc accggaagtg attgaacgcg cgttgaatgg tttgcttatt
    10381 catcgggaaa agttaacccc ggagcatttt acggcgctaa ccgactgctg cccgacacag
    10441 gcgttaaccg tgtgtggtga agtgaaaagc gttgaggaga tcatggcaac cgttttgcgc
    10501 gataaaccgt tttacgatcg cagcggcggc ggcttaacgc tttctggcgg tgaaccgttt
    10561 atgcagccgg aaatggcgac ggcgctgctg caagccagcc acgaggccgg cattcatact
    10621 gcggtagaaa cctgtctgca tgtgccgtgg aaatatatcg ccccttctct gccctatatc
    10681 gatctgtttc ttgccgattt aaaacacgtt gccgacgcgc cgtttaaaca gtggaccgac
    10741 ggtaacgccg ccagggtgct ggataacctg aaaaaactcg ccgcagcggg caaaaaaatc
    10801 attatccgcg tgccactgat tcagggcttt aatgccgacg aaacctctgt gaaagccatt
    10861 accgattttg ccgccgacga gctgcacgtt agcgaaattc atttcctgcc ctaccacacg
    10921 ctgggcatca acaaatatca cttacttaat ctgccctatg acgccccgga aaaaccgctt
    10981 gatgcgccag aactgctcga ctttgcccag cagtatgcct gccagaaagg gttaaccgcg
    11041 accttacgag gataacaatc atgacaacac taaaactgga tacgctcagc gaccgcatta
    11101 aagcgcacaa aaatgcgctg gtgcatattg tgaaaccgcc ggtctgtacc gaacgagcac
    11161 aacactatac cgagatgtat caacaacatc tcgataagcc aatcccggta cgtcgcgcgc
    11221 tggcactggc gcatcacctg gcgaatcgca ccatctggat caaacatgat gaattgatca
    11281 ttggcaacca ggcaagcgaa gttcgcgccg cgccgatctt cccggaatat accgtctcct
    11341 ggatcgaaaa agagattgat gatctggcag atcgtcccgg tgctggtttt gcggtgagcg
    11401 aagagaacaa acgcgttctg catgaagtgt gcccgtggtg gcgcggtcag accgtacagg
    11461 atcgctgcta cggcatgttt accgatgagc aaaaaggtct gctggcgacc ggcatcatta
    11521 aagcggaggg caatatgacc tccggcgatg cgcacctggc ggtcaatttc ccgctactgc
    11581 tggaaaaagg tcttgatggt ctgcgtgaga aagtggcgga acgtcgctcg cgcatcaatc
    11641 tgacggttct ggaagatctg cacggcgagc aattcctgaa agcgattgat atcgtgctgg
    11701 tggcagtcag tgaacacatt gaacgtttcg ctgccctggc gcgtgaaatg gccgcaaccg
    11761 aaacccgcga aagccgtcgc gatgaactgc tgacgatagc agaaaactgc gatcttatcg
    11821 cccaccagcc gccgcagact ttctggcagg cgctgcaact gtgctacttc atccagttga
    11881 ttttgcagat cgaatctaac ggtcactcgg tatcgtttgg tcgtatggac cagtatctct
    11941 acccatacta tcgccgtgac gttgaactga accagacact ggatcgcgaa cacgccatcg
    12001 agatgctgca tagctgctgg ctgaaactgc tggaagtgaa caaaatccgc tccggctcac
    12061 actcaaaagc ctctgcggga agtccgctgt atcagaacgt caccattggc gggcaaaatc
    12121 tggttgatgg tcaaccgatg gacgcggtaa atccactctc ttatgcgatc ctcgaatctt
    12181 gcggtcgcct gcgttcgact cagcctaacc tcagcgtgcg ttaccatgcg ggtatgagta
    12241 acgatttcct cgacgcctgt gttcaggtga tccgctgcgg cttcgggatg ccggcgttca
    12301 acaacgacga aatcgtgatc ccggaattta ttaaactcgg tattgaaccg caggacgctt
    12361 acgactacgc agcaattggt tgtatcgaaa ccgccgtcgg tggcaaatgg ggctatcgct
    12421 gtaccggcat gagctttatc aacttcgccc gcgtgatgct ggcggcgctg gaaggtggtc
    12481 gtgatgccac cagcggcaaa gtgttcctgc cacaagcaaa agcgttgtca gcgggtaact
    12541 tcaacaactt cgatgaagta atggacgcat gggatacgca aatccgttac tacacgcgta
    12601 aatcaatcga aatcgaatat gtcgtcgata ccatgctgga agagaacgta cacgatattc
    12661 tctgctcggc gctggtcgat gactgtattg agcgagcgaa aagtatcaag caaggcggcg
    12721 cgaaatatga ctgggtttct ggcctgcagg tcggcattgc taacctcggc aacagcctgg
    12781 cggcagtgaa gaaactggta tttgaacagg gcgcgattgg tcagcaacag ctggctgccg
    12841 cactggcgga tgacttcgac ggcctgactc acgagcagct gcgtcagcgg ctgattaacg
    12901 gtgcgccgaa gtacggcaac gacgatgata ctgtcgatac gctgctggct cgcgcttatc
    12961 agacctatat cgacgaactg aagcagtacc ataacccgcg ctacggtcgt ggtccgattg
    13021 gcggcaacta ttatgcgggc acatcgtcta tctccgctaa cgtaccgttt ggcgcgcaga
    13081 cgatggcaac gccggacgga cgtaaagcac ataccccgct ggcagaaggc gctagtccgg
    13141 cctccggtac tgaccatctc ggccctactg cggtcattgg ctcggtgggt aaactgccta
    13201 cggcagcaat tctcggcggc gtgctgctca accagaaact gaatccggca acgctggaga
    13261 atgaatctga caagcagaaa ctgatgatcc tgctgcgtac cttctttgaa gtgcataaag
    13321 gctggcatat tcagtacaac attgtttccc gcgaaacatt gctggaggcg aagaaacatc
    13381 cggatcaata ccgcgatctg gtggtacgcg tagcgggtta ttccgccttc ttcaccgcgc
    13441 tctctccaga cgcacaggac gatatcatcg cccgtaccga acacatgctg taatcccctc
    13501 agcccggcgt cgtcgtcgcc gggccaaatc attcacatca tcaatttcag cctcgctttc
    13561 attcgaaata taatttgtgc tctgcgtcac attgttatta cactgttacg gtttcatttt
    13621 cgagccagga gcacacctat gagcgtaaaa gttatcgtca cagacatgga cggtactttt
    13681 cttaacgacg ccaaaacgta caaccaacca cgttttatgg cgcaatatca ggaactgaaa
    13741 aagcgcggca ttgagttcgt tgtcgccagc ggtaatcagt attaccagct tatttcgttc
    13801 tttcctgagc taaaggatga gatctctttt gtcgcggaaa acggcgcact ggtttacgaa
    13861 catggcaagc agttgttcca cggcgaactg acccgacatg aatcgcggat tgttattggc
    13921 gagttgctaa aagataagca actcaatttt gtcgcctgcg gtctgcaaag tgcatatgtc
    13981 agcgaaaatg cccccgaagc attcgtcgca ctgatggcaa aacactacca tcgcctgaaa
    14041 cctgtaaaag attatcagga gattgacgac gtactgttca agttttcgct caacctgccg
    14101 gatgaacaaa tcccgttagt gatcgacaaa ctgcacatag cgctggatgg cattatgaaa
    14161 cccgtcacca gtggttttgg ctttatcgac ttgattattc ccggtctaca taaagcaaac
    14221 ggtatttcgc ggttactgaa acgctgggat ctgtcaccgc aaaatgtggt agcgattggc
    14281 gacagcggta acgatgcgga gatgctgaaa atggcgcgtt attcctttgc gatgggcaat
    14341 gctgcggaaa acattaaaca aatcgcccgt tacgctaccg atgataataa tcatgaaggc
    14401 gcgctgaatg tgattcaggc ggtgctggat aatatcgctc ctttcaatag ttgatcttcc
    14461 ccctggccga agtgaccact tcggcctctc tcctttttgc tcgctttgtg cactgactca
    14521 atatttttat cttgcgttaa cttattttta acttaatgta tcaatcgcaa tcattttact
    14581 ttcgaatgaa agaagcgagg ttgatatgac tttttcctgt gagacgctgc ccacagatgc
    14641 gaaagcagct atccgagaga tgaaagctgc gctgcgggaa cagctcagtg atgtgcaaca
    14701 ggttttcgat acactctctg caaaaatcga aacgcgtgtt gctgaaatcg atgccctaaa
    14761 agcgcaaggg ctgccggttt ggccggaagt ttcttatagt gatattgccg caggtacagt
    14821 aagcgatgcc actcgcaatg aaattaaacg tcgcggttgt gcagtcataa aaggccattt
    14881 ccctcgcgag caggcgatgg cgtgggatcg tgccatgctc gattatcttg atatcaatca
    14941 ttttgatgat gtttataaag gccctggcga cagttttttc ggctcgctgg aggcttctcg
    15001 tcccgaaatc tatcccattt actggtcgca agcacagatg caggcgcggc agagcgaaaa
    15061 catggctgct gtgcaatctt ttctaaaccg actgtggaag tctgaaagca acggcaaaca
    15121 gtggtttaat cctgatatca gtgtgattta tccagacagg atccgccgcc gccctccagg
    15181 tactacatct aaaggcctgg gcgcgcatac cgattctggt gcgctggaac ggtggttatt
    15241 acccgcttat cagcgcgttt ttgccaatgt gtttaatggt aatttcgatg attacgatcc
    15301 ctgggatgct gcacaccgga ccgatgtcga agagtacacg gtagataaca ctacaaaatg
    15361 ttcggtgttc cgcacgtttc agggctggac ggcgctctct gatatgttat cgggtcaggg
    15421 attgttacat gtggtgccta tcccggaagc gatggcgtat gttttattgc gcccgctgct
    15481 ggatgacgta ccggacgatg aactgtgcgg ggttgcacct ggcaaagttc tgcccatttc
    15541 agagaaatgg catccactac tgcttaaagc gctgacttcc attcccgcga taacagcagg
    15601 ggattccgtc tggtggcact gcgatgtgat tcactccgtc gcgccagtag acaatcaaca
    15661 gggttggggc aacgtgatgt atatccctgc tgcgccaatg tgcgagaaaa atcgggctta
    15721 tgcacacaaa gtcaaaatgg cgctggaaaa aggcgcatca cctggcgact tcccgcggga
    15781 agattatgaa gcaaactggg aaggccgctt tacgctggag gatctgaaca ttcacggtaa
    15841 gcgcgcactg ggcatggatg tttgagagtt aatggaagtg aattgatgtg ggattaagaa
    15901 aatcgattca acgttactca gattgctgac aaatgcgctt tgttaatgcc tgatgcggcg
    15961 ttaacgcctt atccggccta caaagtcctg cagattcaat atattgcagg tactgcgtag
    16021 gcctgataag catagcgcat caggcaatct tgcgtttgtc atcagccgcc gtcaccccga
    16081 cagtatcggg ctgacggata ataacttact cgatcccttt actacgcaga taatcttcgt
    16141 aattaccgct aaagtcgatc acgcgttccg gggtgatttc cagaattcgg gtcgccaggg
    16201 agcttacaaa ctcacggtcg tgagacacaa agatcagcgt gccctgatac agttccagcg
    16261 ccatgttcag cgactcaatg gattccatat ccaggtggtt ggtcggttcg tccatgatca
    16321 gaatgttcgg cttctgcatc attaacttac caaacagcat ccgccctttc tccccaccgg
    16381 aaagcacttt cgctggcttt ttgatgtcgt cctggctgaa cagcaaacga ccgagaatgc
    16441 tgcgtaccgc ctgctcgtca tcgccttcct gcttccactg actcatccat tcgaacacgg
    16501 taagatcatt ttcgaactca tattcgtggt cctgggcgta gtaaccaatg cgcgcgtttt
    16561 cagaccattt tacggtgccg ctgtccggtt gcagatcgcc caccagcgtt ttcaacagtg
    16621 ttgatttacc gacgccgttg gtacccagta ccgccagttt ttcaccgact tccagcagca
    16681 ggttgagatt tttaaacagc ggaccgttat caaacccttt ggtcagacct tccacttcca
    16741 gcgcgttacg gaacagtttc ttatcctgtt caaaacggat gaacgggttc tgacggctgg
    16801 aggctttcac ctcttccagt ttgattttat caatctggcg cgcgcgcgaa gttgcctggc
    16861 gagatttcga ggcgttggca ctaaagcggc taacgaaaga ttgcagctca gcaatctgcg
    16921 ctttcttctt ggcgttatca gccagcagac gctcacgcgc ctgggtcgcc gccgtcatgt
    16981 actcatcgta gttacctgga taaacgcgca gctcgccgta atccagatcc gccatgtggg
    17041 tacaaaccat gttaaggaag tgacggtcgt gcgagatgat gatcatggtg ctgtcacgct
    17101 cgttcagcac ctgttccagc caacgaatgg tgtcgatgtc gaggttgttg gtcggttcgt
    17161 cgagcaggag aatgtccgga tcagcaaaca gcgcctgcgc cagcagcaca cgcagcttcc
    17221 agccaggagc aacttcactc atcgggccgt agtgctgttc cactggaatt cccacgccaa
    17281 gcaacagttc accggcgcga gcttccgcag agtaaccgtc catttcgccg tatttcactt
    17341 ccagatcggc cactttatag ccgtcttctt cactcatttc cggcaaagca tagatgcggt
    17401 cacgctcctg cttcacttcc cacaactctt tatgcccaat gatcaccgta tccagcacag
    17461 tgaactcttc aaaggcaaac tgatcctgac gcagcttacc aatgcgctcg ttgggatcga
    17521 gggaaacgtt acccaacgtc ggctctaagt cgccgccgag gatcttcata aaggtggatt
    17581 taccactacc gttcgcgcca atcaggccgt aacggttgcc gccgccaaat ttgacggaaa
    17641 tgttttcaaa caacggctta ctgccgaact gcatggtgac gttactggaa actaacacag
    17701 gcgtatcctg aaaagagata tgacaaaccg cgcattatgc cataaatctg aaagaagatc
    17761 ctgctcctcg cttaaggtct acactcaagc caaaggcaaa aaatgtgatt tcgtacacat
    17821 ctgatttcac tgtgagctgg aatgaactta taatgcgctt ccaatactct aatattctca
    17881 acccaatggc ctgccaggca caaaatctcg cttaacatga atatgaaatt gaaaacatta
    17941 ttcgcagcgg ccttcgctgt tgtcggcttt tgcagtaccg cctctgcggt aacttatcct
    18001 ctgccaaccg acgggagtcg cctggttggt cagaatcagg tgatcaccat tcctgaaggt
    18061 aacactcagc cgctggagta ttttgccgcc gagtaccaga tgggactttc caatatgatg
    18121 gaagcgaacc cgggtgtgga taccttcctg ccgaaaggcg gtactgtact gaacattccg
    18181 cagcagctga tcctgccgga taccgttcat gaaggcatcg tcattaacag tgcagagatg
    18241 cgtctgtatt actatccgaa aggaaccaac accgttatcg tgctgccgat cggcattggt
    18301 cagttaggca aagacacgcc tatcaactgg accaccaaag ttgagcgtaa aaaagcaggc
    18361 ccgacctgga cgccgaccgc caaaatgcac gcagagtacc gcgctgcggg cgaaccgctt
    18421 ccggctgtcg ttccggcagg tccggataac ccgatgggtc tgtatgcact ctacatcggt
    18481 cgcctgtacg ctatccatgg caccaacgcc aacttcggta tcggcctgcg tgtaagtcat
    18541 ggctgtgtgc gtctgcgtaa cgaagacatt aaattcctgt tcgagaaagt gcctgtcggt
    18601 actcgcgtgc agtttattga tgagccggtg aaagcgacta ccgaaccaga cggcagccgt
    18661 tacattgaag tccataaccc gctgtctacc accgaagccc agtttgaagg tcaggaaatt
    18721 gtgccaatta ccctgacgaa gagcgtgcag acagtgaccg gtcagccaga tgttgaccag
    18781 gttgttcttg atgaagcgat caaaaatcgt tccgggatgc cggttcgtct gaattaatcg
    18841 tcaaaacgta aaacaaaggc ggactcataa tccgcctttt ttatttgcca gaccctacca
    18901 ggccgggatc ataaccaaca aaacatatcc caccagcgcc gcccacaaca gcatcggtat
    18961 cgaataaaga tgaaaacgcc accagatacg gcgatcgttc gccatccgta acgcaatcaa
    19021 atttgccagc gatccgggta ataaaccaaa gccccctacg tttaccgccc atgccagcag
    19081 caacgacggc ggcacatagt tcagcaacaa tatggtactc ggcacgttac tgatcacctg
    19141 cgataaaccg attgccgtta accataatcc gggttcggat aaatgactca cgttacttaa
    19201 caccccctgc aaagctggca gttgggttag taaatggacg tcgataaaca tcgccataaa
    19261 caccagcagc agcgtccagt ccacactgag caccacgcga cgtgccagca gcgcaaagcc
    19321 caccgccaca atcaccagtc cccacagctc ttgttttaac tccagcgcca tcagaaagac
    19381 gatatacagc cccagacaac tccacaccag ccgcggtttc cactccggcg tttgcgcccc
    19441 cgtatggtat tgcagcgcct ttccagggaa acaacaccag cacaacagca ggagtgtcag
    19501 catcattgcg ccagccagcg gtgccatttg ggcaataaat ccggcaaacg aaagaccaga
    19561 acgtccccag ataagaatat tttgcgggtt gccaattggc gtcagtagcg aaccagcgtt
    19621 gactgccagc gcctcaaaaa taatcagccg attaaccggg atctcacaca gtctttttag
    19681 cgtgatagtc agcggaacaa caataaacag cgcgacatcg ttggtcagaa aggtagaaag
    19741 cagcgccgcc gccagcacca taaacatcgc cagccgacgc tccgtagcaa agcggcgcac
    19801 cattttgcgc cccagcacat caaaataacc gcttaactcc acacctttgg tcagcagcat
    19861 caggccgctt aaggtgatga tggtgtgcca gtcgatagca gcaggccagg atttcggtgc
    19921 aaagggaacg aaaaaactta atccgatgcc aacaagaatt aataactgaa aaaaacgatc
    19981 gccttgcagc gtgcgtaaaa aaggcaggct catttggcac cgtgtttctg ggtaaacaaa
    20041 cgaaaggcat ccagcgtctc ttcactaaca tggtgctcca tgccttccgc gtcgcgacgg
    20101 gcgatttccg gactgacgcc caacaccaac aagaaatttt cgactatctg atgtcgctcg
    20161 cggctttcct gcgccagttt ctctccttct gccgttaaaa acacgccacg ccaggggatc
    20221 atttcaatca gccccatggt tgccagtcgc ttaagcattt tagccaccgt cggttgcgaa
    20281 actcccaaac gagcagccat gtatacctga cgagcttccc ccacttccct gatcaagtca
    20341 gaaatcagct caacgtaatc atcaatcagc tcgcgccgat gcgcctctct gacctggcgg
    20401 aacccttcaa cgtgctcttc cacgttcact aactgcgtca ctttttttgc tgttggcgta
    20461 cctgcgcgac gactcattgt gcttcctcat tatggtgacg catacatagc gttctcattt
    20521 cgtgcgcaca ttgtaaacca gagttgcgaa ggtacaaaaa attaacgttt tagcaatagc
    20581 tatataatat agcctgtgct atatctgtat gtaatgcaat catccctcaa ggatcgacgg
    20641 gattagcaag tcaggaggtc ttatgaatga gttcaagagg tgtatgcgcg tgtttagtca
    20701 ttctcccttt aaagtacggt taatgctgct ctctatgttg tgcgatatgg tcaacaacaa
    20761 accgcagcaa gataaacctt cagataaata gcggcgtcgc ggtacgccgc ttcactcctg
    20821 cttttatgca ggcataacgc gttttggtct gaaaaacccc actttttgtc ggatttgcaa
    20881 tccccttcgc aaaagatttg ttcgtcagta gttgacctga acggcggctc gctctatctt
    20941 cttgcagccc tgcgtatatt gcggctcgcg gatgcggacc cctttcccct ctccacgcac
    21001 tcttgcaggt attgaccctt gacgccaagg taagcacatg gcgtttgtta cgatagtggc
    21061 atatgaattt aaccctcaaa gaatcgcttg ttacccgtag ccgggtattt agcccgtgga
    21121 ctgcgttcta ctttttacag tcgctattaa ttaacctcgg cttaggttat cccttcagtt
    21181 tgctctacac cgctgcgttt acggctattt tgcttttgct atggcgaaca ttgcctcgcg
    21241 tacaaaaagt tctggtcggt gtcagttcgc tggtggcggc ttgttatttc ccttttgctc
    21301 aggcctacgg cgcacctaat ttcaatacat tgctggcatt gcactccacc aatatggaag
    21361 agtcgaccga aatcctgacg atttttccgt ggtacagcta cctggtcggc ttatttattt
    21421 ttgcgctcgg cgtaatagca atcaggcgga aaaaagagag tgaaaaagcg cgctggaata
    21481 ccttcgacag cctgtgtctg gtattcagtg tggcgacatt ttttgttgct cccgtgcaaa
    21541 acctggcctg gggtggcgta tttaaactga aagatactgg ctatccggta tttcgttttg
    21601 ctaaggatgt catcgtcaat aataacgagg tgattgaaga gcaagaacgg atggcaaaac
    21661 tgtccggaat gaaagatacc tggacggtca ctgccgttaa gccgaagtat cagacctatg
    21721 tggtggtgat cggagaaagc gcgcgtcgcg atgccctcgg tgcctttggc ggtcactggg
    21781 acaatacccc gtttgccagc agcgttaacg gtttgatatt tgctgactac attgccgcca
    21841 gtggctccac gcagaaatcg cttggcttaa cgctcaatcg cgttgtcgat ggcaaaccac
    21901 agtttcagga taactttgtc accctggcaa atcgcgcggg cttccagacc tggtggtttt
    21961 ccaaccaggg ccaaatcggc gaatacgata ccgccatcgc cagcatcgcc aaacgtgcag
    22021 atgaagtgta cttcctgaaa gaaggtaatt ttgaagcaga taaaaacacg aaagacgaag
    22081 cgttactgga tatgaccgct caagtgctgg cgcaagagca ctcgcaaccg cagctgattg
    22141 ttttacatct gatgggctca catccgcagg cctgcgaccg gacacaaggc aaatacgaaa
    22201 cctttgtgca atcgaaagaa acgtcgtgct atctctatac catgacgcaa acagacgatt
    22261 tactgcgtaa gctgtacgat cagttacgca acagcggcag tagcttctcg ctggtttact
    22321 tttctgacca cggtctggcc tttaaagagc gtggcaaaga cgtgcaatac cttgcccatg
    22381 atgataagta tcagcaaaat ttccaggtgc cttttatggt catttcgagc gacgataaag
    22441 cgcatcgggt gattaaagcc cgccgctctg ccaatgactt cttaggcttt ttctcccagt
    22501 ggacgggaat taaagcgaag gaaataaata tcaaataccc gtttatatct gagaagaaag
    22561 ccgggccgat atacatcacc aacttccagt tacagaaggt agattacaac catctcggaa
    22621 ccgatatttt cgacccgaaa ccttaaaaac aaaaatccgc cccgagaggc ggatttttta
    22681 tatcaccgaa gtgattagaa gcggtaacca acaccggcaa tccaggtgcc tacgtcaacg
    22741 ctacgaatac ggctctgctc gtaagagaag tccagagcaa cgttttccat cgggttgaac
    22801 tgcagaccag caccgtagga gaaaccgtag tcgctggtgt cgtgtttgta ggtcgggtat
    22861 tcagtggtct ggaatttacc ataacccaca cccactacac cgtagatgct tgcccagtcg
    22921 ttaatgcggt aagccggacc agcagtgatg ccgtagtact ggtttttgtt gtagtcacca
    22981 gagcttgcag tacggctttt ctcggtgtaa gtgaaagaac cgatcacacc cagcgggctg
    23041 ttgtcttctt catagcggta tttcaggttg aaaccgccca ttttgttcat ttggccctga
    23101 gcgtcgctct gtgcgtaacc gccagttacg gtagaagtcg cagctacgga agtacctgcg
    23161 gtgaaagcca gaactgcggc cagtgctgaa agacatgcaa tttttttcat aaccacctca
    23221 aatgtgattc aaataagtcc taagttttaa atatatcaaa aattaatggg aaactcttcg
    23281 cgatttgtga tgtctaacgg gccatttcat gtaacagaac gtttccatac accgctatcc
    23341 atctaaattt aaatcacttt ttcagagagc tgcataagta ttacgcatgt tttccctgtc
    23401 attcatccag attattccta atcaccagac taatgattcc atcaatcctg gcgcatttta
    23461 gtcaaaacgg gggaaaattt tttcaacaaa tgctcaacca gcattgggta tatccagtac
    23521 actccacgct ttacttaggt ttagatattt gtgggagaaa ggatgcctgg ttcattacgt
    23581 aaaatgccgg tctggttacc aatagtcata ttgctcgttg ccatggcgtc tattcagggt
    23641 ggagcctcgt tagctaagtc actttttcct ctggtgggcg caccgggtgt cactgcgctg
    23701 cgtctggcat taggaacgct gatcctcatc gcgttcttta agccatggcg actgcgcttt
    23761 gccaaagagc agcggttacc gctgttgttt tacggcgttt cgctgggggg gatgaattat
    23821 cttttttatc tttctattca gacggtaccg ctgggtattg cggtggcgct ggagttcacc
    23881 ggaccactgg cggtggcgct gttctcttct cgtcgcccgg tagatttcgt ctgggttgtt
    23941 ctggcggttc ttggtctgtg gttcctgcta ccgctggggc aagacgtttc ccatgtcgat
    24001 ttaaccggct gtgcgctggc actgggagca ggggcttgtt gggctattta cattttaagt
    24061 gggcaacgcg caggagcgga acatggccct gcgacggtgg caattggttc gttgattgca
    24121 gcgttaattt tcgtgccaat tggagcgctt caggctggtg aagcactctg gcactggtcg
    24181 gttattccat tgggtctggc ggtcgctatt ctctcgaccg ctctgcctta ttcgctggaa
    24241 atgattgccc tcacccgttt gccaacgcgg acatttggta cgctgatgag catggaaccg
    24301 gcactggctg ccgtttccgg gatgattttc ctcggagaaa cactgacacc catacagctg
    24361 ctggcgctcg gcgcaattat cgccgcttca atgggttcta cgctgacagt acgcaaagag
    24421 agcaaaataa aagaattaga cattaattaa atttacattt ctgcatggtt atgcataacc
    24481 atgcagaatt tatcgctact tttcctctac accgtcttta tatatcgaat tatgcaaaag
    24541 catatttatt ccgaaaattc ctggcgagca aataaataag aattgttctt attaatatat
    24601 ctaactcatt gagtttttat tggttttgtt ttttcacgct tgttaccact attagtgtga
    24661 taggaacagc cagaatagcg gaacacatag ctggtgctat acttagtctc gttaattact
    24721 gggacataac atcaagagga tatgaaatta tgagtaccgc taaattagtt aaatcaaaag
    24781 cgaccaatct gctttatacc cgcaacgatg tctctgatag cgagaaaaaa gcaacagtag
    24841 agttgctgaa tcgccaggtt atccagttta ttgatctttc tttgattacc aaacaagcgc
    24901 actggaacat gcgcggcgct aacttcattg ccgtacatga aatgctggat ggcttccgca
    24961 ctgcactgat cgatcatctg gataccatgg cagaacgtgc agtgcagctg ggcggtgtag
    25021 ctctggggac cactcaagtt attaacagca aaactccgct gaaaagttac ccgctggaca
    25081 tccacaacgt tcaggatcac ctgaaagagc tagctgaccg ttacgcaatc gtcgctaatg
    25141 acgtacgcaa agcgattggc gaagcgaaag atgacgacac cgcagatatc ctgaccgccg
    25201 cgtctcgcga cctggataaa ttcctgtggt ttatcgaatc taacatcgaa taaatccatc
    25261 gctgatggtg cagaacttta gtacacgata aaggcggctt cctgacagga ggccgttttg
    25321 ttttgtagcc cgcctcaacg cacttattta gtgcatccat ctgctatctt cagctgatta
    25381 agtaaatttt ttgtatccac atcatcacac aatcgttaca taaagattgt tttttcatca
    25441 ggttttacgc taaataatca ctgtgttgag tgcacaattt tagcgcacca gtttggtgcc
    25501 ccagaatggt gcatcttcag ggtattgccc tataaatcgt gcatcacgtt tttgccgcat
    25561 ctcgaaaaat caatgagttg caaaactggc acgatttttt catatatgtg aatgtcacgc
    25621 aggggatcgt cccgtggata gaaaaaagga aatgctatga agtctgtatt aaaagtttca
    25681 ctggctgcac tgaccctggc ttttgcggtt tcttctcatg ccgcggataa aaaattagtt
    25741 gtcgcgacgg ataccgcctt cgttccgttt gaatttaaac agggcgataa atacgtgggc
    25801 tttgacgttg atctgtgggc tgccatcgct aaagaactga agctggatta cgaactgaag
    25861 ccgatggatt tcagtgggat cattccggca ctgcaaacca aaaacgtcga tctggcgctg
    25921 gcgggcatta ccatcaccga cgagcgtaaa aaagcgatcg atttctctga cggctactac
    25981 aaaagcggcc tgttagtgat ggtgaaagcc aacaataacg atgtgaaaag cgtgaaagat
    26041 ctcgacggga aagtggttgc tgtgaagagc ggcactggct ccgttgatta cgcgaaagca
    26101 aacatcaaaa ctaaagatct gcgtcagttc ccgaacatcg ataacgccta tatggaactg
    26161 ggcaccaacc gcgcagacgc cgttctgcac gatacgccaa acattctgta cttcatcaaa
    26221 accgccggta acggtcagtt caaagcggta ggtgactctc tggaagcaca gcaatacggt
    26281 attgcgttcc cgaaaggtag cgacgagctg cgtgataaag tcaacggcgc gttgaaaacc
    26341 ctgcgcgaga acggaactta caacgaaatc tacaaaaaat ggttcggtac tgaaccgaaa
    26401 taataacgct acgcctgtaa ggcgcactgg cagttccctc tcccctctgg ggagaggatt
    26461 agggtgaggg gcgcaaaccc gctccggggc cattaattac cctgaatttg attatttaca
    26521 ccacggtaac aggaacaaca tatgcagttt gactggagtg ccatctggcc tgccattccg
    26581 cttctgattg aaggtgccaa aatgaccctg tggatctcgg tcctcggtct ggctggcggc
    26641 ctggtgatcg gattgctggc aggttttgca cgcaccttcg gtggttggat agccaaccac
    26701 gtcgcgctgg tctttattga agtgatccgc ggcacaccta tcgtcgtcca ggtgatgttt
    26761 atctatttcg ccctgccgat ggcatttaac gacttacgca tcgacccatt tactgcggcg
    26821 gtggtcacca tcatgatcaa ctccggcgca tacattgcgg aaatcacgcg tggtgcggtg
    26881 ctgtctattc acaaaggttt tcgtgaagca ggactggcgc tcggtctttc acgttgggaa
    26941 accattcgct acgtcatttt accgctggca ctgcgtcgta tgctgccgcc gctgggtaac
    27001 cagtggatca tcagcattaa agacacctcg ctgtttattg ttatcggcgt ggcggaactg
    27061 acccgtcagg ggcaagaaat tattgccggt aacttccgcg cccttgagat ctggagcgcc
    27121 gtggcggtgt tctatctgat tattaccctg gtgctgagct ttattctgcg tcgtctggaa
    27181 agaaggatga aaatcctgtg attgaattta aaaacgtctc caagcatttt ggtccaaccc
    27241 aggtgctgca caatatcgat ttgaacattg ctcagggcga agtcgtggtg attatcgggc
    27301 cgtccggttc cggtaaatcg accctgctgc gctgcatcaa caaactggaa gaaatcactt
    27361 ccggcgatct gattgtcgat ggcctgaagg ttaacgatcc gaaagttgac gagcgcctga
    27421 ttcgccagga agcaggtatg gtgttccagc agttttacct cttcccgcat ctgacggcgc
    27481 tggaaaacgt catgtttggt ccgctacgcg tgcgtggcgc gaacaaagaa gaggcggaaa
    27541 aactggcgcg tgagctgctg gcgaaagtcg gtctggcaga acgtgcacat cactaccctt
    27601 ccgaactttc tggtggtcaa cagcagcgtg tggcgatcgc acgcgcgctg gcggtgaagc
    27661 cgaaaatgat gctgtttgat gaaccgactt ccgctcttga cccggaactg cgccatgaag
    27721 tgctgaaggt tatgcaggat ctggctgaag aagggatgac gatggtgatc gtgacacacg
    27781 aaatcggttt tgccgagaaa gtagcttcgc gcctgatctt tatcgacaaa ggccggattg
    27841 cggaagatgg cgatccgcag gtgttgatca agaacccacc gagccagcgc ttgcaggaat
    27901 ttttgcagca cgtctcttaa cttctcagcc tccctttcct ccgaaaggga ggcacaccag
    27961 attcctctca tttaaaatcg cccctcctcc agcatctata cttatctttt tgctctgttt
    28021 tctcactgga ggagtcatgc ggtggatcct gttcatcctc ttctgcctgc tgggcgcacc
    28081 tgcccacgcg gtatccatac ccggcgttac aaccacaacg acaacggact caacgactga
    28141 accggccccg gaaccggata tcgaacaaaa aaaagcggcc tatggtgcac tggcggatgt
    28201 gctggataat gacacctcac gtaaagagtt gatcgaccag ttgcgcaccg tcgccgcgac
    28261 gccccctgcg gaacccgtac cgaagatcgt gccgccgacg ctggttgaag agcaaaccgt
    28321 gctgcaaaaa gtcaccgaag tcagccgcca ttatggtgaa gccctttccg cccgcttcgg
    28381 acaactttat cgcaatatca ccggctcccc gcataagccg tttaatccac aaaccttcag
    28441 caatgcgttg acccattttt caatgttagc ggtattagtg tttggttttt actggctgat
    28501 tcgcctgtgc gcacttccgc tgtatcgcaa aatgggccag tgggcgcggc aaaaaaatcg
    28561 tgagcgcagt aactggttgc agcttccggc gatgattatc ggggcgttta ttatcgacct
    28621 gctgttactg gcactgacat tatttgtcgg tcaggtttta agcgacaacc tgaatgcggg
    28681 cagtcgcacc atcgctttcc aacaaagttt gtttctcaac gcctttgccc tgattgaatt
    28741 tttcaaagct gtactacgcc tgattttttg cccaaatgtg gcggagctgc gcccgttcac
    28801 gattcatgac gagaccgccc gttactggag ccgtcgcctg agctggttaa gcagtttgat
    28861 aggttatggc ctgattgtgg ccgtgccgat tatctctaat caggtgaatg tacaaattgg
    28921 cgcgctggcg aacgtcatca ttatgctgtg catgaccatc tgggcgttgt acctgatctt
    28981 tcgtaataaa aaagagatta cccaacattt gctcaacttt gcggagcatt cgctggcctt
    29041 tttcagcttg tttatccgcg cctttgcgct ggtgtggcac tggctggcaa gcgcctattt
    29101 tatcgtgctg tttttctttt cgttgttcga tccgggcaac agcctgaaat ttatgatggg
    29161 tgcaacggtg cgcagcctgg cgattattgg tatcgcagca tttgtttccg gtatgttttc
    29221 ccgctggctg gcgaaaacca tcaccctctc gccacatact cagcgaaatt atccggagct
    29281 gcaaaaacgg ctgaatggct ggctttcggc ggcgctgaaa acggcgcgta ttctgaccgt
    29341 ctgcgtggcg gtaatgctgc tgttgagcgc atggggatta ttcgatttct ggaactggct
    29401 gcaaaacggt gcggggcaga aaaccgtaga tatcctgatc cgtatcgcac tcattctttt
    29461 cttctcggcg gttggctgga cggtgctcgc cagtttgatc gaaaaccggc tggcttcgga
    29521 tattcatggc cgcccactac ccagcgcccg tacgcgtacc ctgctgacgc tgttccgtaa
    29581 cgcgctggcg gtgattatca gtaccatcac catcatgatt gtgttgtcgg aaatcggcgt
    29641 taatatcgcg ccattgctgg caggtgccgg ggcattaggt ctggctatct cttttggttc
    29701 gcaaacgctg gtgaaagata ttatcaccgg ggtatttatt cagtttgaaa acggcatgaa
    29761 cactggagat ttggtgacta tcgggccgtt gaccggcact gtggaacgga tgtcgattcg
    29821 ctccgtgggc gtgcgccagg ataccggggc gtatcacatc attccgtggt cttcgataac
    29881 cacctttgct aacttcgtcc gcggtattgg ttcggtagtg gcaaactacg atgttgatcg
    29941 ccatgaagat gctgataaag ccaatcaggc actgaaagat gcggtagcag aattgatgga
    30001 aaacgaagaa attcgcgggc tggttattgg tgaaccgaac tttgccggga ttgtcggctt
    30061 aagcaatacc gcgtttacac tgcgtgtttc gttcaccacg ctgccactca aacagtggac
    30121 ggtccgcttt gccctcgaca gccaggtgaa aaaacatttc gacctggcgg gtgttcgcgc
    30181 gccggtgcag acttatcagg tgctgccagc tccgggcgcg gccccggctg aaccgttgcc
    30241 gccgggggag ccaacgcttt aacgctggcg attgacaaaa cggcggcgct gctcgtcgtt
    30301 cataaaggtc caggctataa agcggctctg cttttgcccc tgggccatct ctttttttac
    30361 caccttcacc gcacctacgt ccgtcaaagc acgatacaac ggcggtaagt tttcaccacg
    30421 agataccagc gaggtaaacc acatcacctg cttcgcgaag cctttgctct cttcaatcat
    30481 ctttttgata aaagcgacct caccgccttc acaccacaac tcctgttgct gaccaccaaa
    30541 gttcagtgca tcgtctttgt tcagccccag gttacggcgt ttacgttcac tacctgcccg
    30601 tgccgcagcg gcggaatcgt ggaatggcgg gttacacaag gtcgcatcgt attgctcgtt
    30661 tttatggatg atgccgttaa aaatcgcccc actctctttt tgccgacgca gacgaatggc
    30721 gcggttaagc cccggattag cactgatgat cgcctgcgca ctgcttaacg cctggctgct
    30781 ggtttcacta ccggtaaaac gccagccata ttcatgtacg ccaatcagcg gataaataca
    30841 gttcgcacca acaccgatat ccagaatgct ggcattagct ggaattgttc cgcttgcttc
    30901 agccagtaaa tcggcaaggt gatgaatata atccgcccgg cccggtactg gcgggcagag
    30961 aaaaccgtcc gggatatccc agttcgctac ggcgtaaaaa tgggccagca acgccttatt
    31021 gagcgccttc accgccagcg gattggcaaa gtctacgctt tgctccccgg cgggtgtaag
    31081 cgtgaggaat tgcctgagtt caggattgac ctgacaaagc gtggcgagat cgtagcggct
    31141 gtgatgacgg ttgcgcggat gcaaccccgg tttctgggcg gacatagcat tctcctttaa
    31201 gcagcggcgt aagatacccg cataacgcga cagggtaaat atctggctgc gtttatcatt
    31261 tctaaaaacc ttcacatggc tatggtttat ggtaattcac cataaaaatc atcatcatgc
    31321 atgtcattga tttatcttat tttgtaaaaa tatattgttc aaaaaataag cgcatctctg
    31381 tcaatttttt gcgtcatttt catcgcgcag gcgacctaca cttatctggc actacccgtt
    31441 gtatttatga ggatgtcatt atgaaaaagt gcctcacact actgattgcc accgtcctga
    31501 gcggaatctc tctcacggct tatgccgcgc aaccgatgag taacctggac agtggtcaac
    31561 tacggcccgc cggtaccgtt tcggcaactg gcgcatcaaa cctaagcgat ctggaggata
    31621 aactggcaga aaaagcgcgc gaacaaggcg cgaaaggtta tgtcattaat tccgcaggcg
    31681 gaaatgacca gatgttcggt actgcaatca tctacaaata accccaccaa cgtaactgca
    31741 ttcgcctctg gatgcagttt tcgtccaatt tgtaagcaaa aaacatcaaa tatgaacgca
    31801 atgtaaataa atgtatttct ttttcgcgca atgggtgata gaaaatcgcg ccaagtgata
    31861 atgcttatca aaattattat cacttgcacg gacgctatca cgggattaac agtggcatcg
    31921 catccgcaga gatgctttct cgtgacagtg aaaatttcac catataagaa aaagtcacct
    31981 gcaaaatgga aaacaatcgc aatttccctg ccagacaatt tcattcgctc acgttctttg
    32041 ccggtctttg tattggtatt acgcctgtgg cgcaggcact cgctgccgaa gggcaagcta
    32101 acgcggatga cacgctggtt gtcgaagcat caacgccttc gctttatgcg ccacaacaat
    32161 ctgccgatcc gaaattctcg cgtccggtag cggatactac ccgcacgatg acggtgattt
    32221 ctgaacaagt gattaaagat cagggtgcaa ccaaccttac cgacgcgctc aaaaacgtcc
    32281 ccggcgtggg tgcgtttttt gcgggtgaga acggtaactc caccactggc gacgccattt
    32341 atatgcgcgg tgccgatacc tctaacagta tttatatcga tggcattcgc gatatcggca
    32401 gcgtctcgcg cgacaccttc aataccgagc aggtcgaagt aattaaaggg ccgtccggca
    32461 ccgactacgg gcgcagcgcg ccgacaggct cgatcaatat gatcagcaaa cagccgcgca
    32521 atgattccgg cattgacgcc tccgccagta ttggcagcgc ctggttccgc cgcggcacgc
    32581 tggacgtcaa tcaggtcatt ggtgatacca ctgcggtgcg cctgaatgta atgggcgaaa
    32641 aaacgcacga tgccggacgc gacaaagtca aaaatgagcg ttacggcgtc gccccttctg
    32701 tcgcttttgg ccttggtaca gcgaatcgtt tgtatcttaa ttatctgcat gtcacccagc
    32761 acaacacgcc agacggcggc attccgacta tcggtttacc gggttattct gccccatctg
    32821 cgggaacagc ggctctgaat cattccggaa aagttgatac tcataacttt tacggcacgg
    32881 attccgatta cgacgattcg accaccgaca ccgctaccat gcgttttgag cacgacatca
    32941 acgataacac caccattcgc aatactaccc gttggtcgcg cgtgaagcag gattacctga
    33001 tgacggcgat tatgggcggg gcgtcgaata ttacccagcc caccagcgat gtgaatagct
    33061 ggacctggtc acgcacggcg aatactaaag atgtgagtaa taaaattctc accaaccaga
    33121 ccaacctgac ctcgacgttc tataccggcg ctatcggtca tgatgtcagt accggcgtgg
    33181 aatttacccg tgaaacgcag actaactacg gcgttaatcc ggtgacgtta cctgcggtaa
    33241 atatttatca tcctgacagc agcattcatc ccggcggcct gacgcgcaac ggcgcaaacg
    33301 ccaatggtca gacggatacc ttcgcaattt acgccttcga tacgctgcaa atcacccgtg
    33361 attttgagct gaacggcggg atccgtctgg ataattatca tactgaatat gacagtgcca
    33421 ccgcctgcgg cggcagcgga cgcggtgcca tcacctgccc tgctggcgtg gcaaaaggtt
    33481 ctccggtcac caccgtcgat accgccaagt cgggcaatct ggtgaactgg aaagccgggg
    33541 cgctgtatca cctgacggaa aacggcaatg tctatattaa ctatgccgtt tcccagcagc
    33601 ctccgggcgg caacaacttc gcccttgcgc agtctggcag cggtaacagt gccaaccgca
    33661 ccgattttaa accgcaaaaa gccaacacca gcgagattgg caccaaatgg caggttctgg
    33721 ataaacgcct gttgctcacc gccgcgctgt tccgtactga tatcaaaaat gaagttgagc
    33781 aaaatgatga cggaacttac tcgcaatacg gtaagaaacg cgtcgaaggc tatgagatat
    33841 ccgtggccgg gaatatcact cccgcgtggc aggtgattgg cggctatacc cagcaaaaag
    33901 caaccatcaa aaacggcaaa gatgttgccc aggatggttc ctcatcgctg ccgtataccc
    33961 cggagcacgc ctttacctta tggagccagt atcaggcaac cgatgatatt tccgttggtg
    34021 cgggcgcacg ctatatcggc agtatgcata aaggttctga cggcgcggtg ggaacgccag
    34081 cgtttaccga aggttattgg gtcgccgacg ctaaactggg gtatcgggtt aatcgcaatc
    34141 tcgacttcca gctaaacgtc tacaacctgt ttgataccga ttacgtcgcc tcaatcaaca
    34201 agagcggcta ccgttatcac ccgggcgagc caagaacctt cttgctcacg gccaatatgc
    34261 atttctgatt gagatgtggg gcgcaggccc cactttttgg agaacttgta tgatgtacca
    34321 cattcccggc gtgctatcgc cacaggacgt cgcccgtttt cgcgaacaac tggaacaagc
    34381 cgaatgggtg gatggacgcg tcaccactgg cgcacaaggt gcgcaagtta agaacaatca
    34441 acaggtagac acccgcagca cgttatacgc cgccctgcaa aatgaggtgc tgaacgcggt
    34501 taaccaacat gctttgttct ttgccgcggc cttgccgcgt accctttcca cgccgctgtt
    34561 taatcgctat cagaacaatg aaacctatgg ttttcatgtg gatggcgcag tacgcagtca
    34621 cccacaaaac ggctggatgc gcactgacct ttctgccacg ctgtttttaa gcgatccaca
    34681 aagctacgat ggcggcgaac tggtcgttaa tgacaccttc gggcaacatc gggtaaaact
    34741 tccggcaggc gatctggtgt tgtatccctc cagcagcctg cattgcgtga cacccgtaac
    34801 ccgcggcgta cgagtggcat catttatgtg gatccagtcg atgatccgcg atgataaaaa
    34861 gcgcgccatg ctgtttgaac tggacaacaa tattcagtcg ctgaaaagcc gctacggcga
    34921 aagtgaagag atcctgtcgc tgctcaatct ttatcataat ctgctgcggg aatggtcgga
    34981 gatctgacgc ttgaactgcc cgagttcgtc cctatattag ctacacttaa ctgtacaagt
    35041 attgatatgg ggaggacgat atggcatccg gttgggctaa cgatgacgcc gtcaacgaac
    35101 agatcaacag tacaattgaa gatgcgattg cccgcgctcg gggtgaaatt ccgcgtggcg
    35161 aaagcctgta tgaatgtgaa gagtgcggag cccccatccc gcaagcccgt cgggaagcca
    35221 ttcctggtgt gcgcttatgc attcattgtc agcaggagaa agatttacaa aaaccagctt
    35281 atacaggata taatcgcaga ggttcgaaag acagccagtt acgttaacta caactggcgg
    35341 gatgtatagc gagtataatt tgtattttgc gtggcattcc gtgcctttaa cgctgtcgtg
    35401 actgccacgc tgcataaaac aagtccataa gttcaacaaa taaatggatt atcagtatgt
    35461 tagatatcaa tcaatttatt tgaacaaggc ggtcaattct cttcgatttt atctctcgaa
    35521 aaaaaacgtg atactcatca catcgacgaa acaacgtcac ttatacaaaa atcacctgcg
    35581 agagattaat tatgaaaact atcaatactg ttgttgctgc tatggctctt tcaactctgt
    35641 catttggcgt gtttgccgcg gaaccggtaa cggcatccca ggcacagaac atgaataaaa
    35701 tcggcgtggt ttctgccgat ggcgcatcca ccctcgatgc cctggaagcg aaactggctg
    35761 agaaagctgc ggcagctggg gcgagtggat acagcatcac ttctgccacc aacaacaata
    35821 aattaagcgg tactgcggta atttataagt aattcgtaga acggataagg cgtttacgct
    35881 acgtccagca caagtatttc cctgtctgtt tgccgacaga cgcatatgct ctaaccctca
    35941 ttgatcctat gttacccttg tttgcccgtc cgccactgga cgggcttttt tttagctggc
    36001 taactgctga cagaaagcct gcaacgtctc ttccggcata ccaacctttc gcgccgcatc
    36061 acaaatggcc tgccagcttc ccgcatccaa tggaatcccc tgctcctgcc gttcgcgacg
    36121 cgtgttaact tcccactcgc ccggtagcaa aatcggctta tcgtcatcat gcggcgaggc
    36181 tttcacccac tcggcaaagg cttcggtctg cgcgctacaa tccggcgcgc cgaacagttc
    36241 cgggttgatg atgatagtgg tcatgcagtt aagaatggca tcgggactgg tttgtaacgt
    36301 tttctgatgc gtcgttttac cgccggaaag cgccccacca agaatttcac acatcgctgc
    36361 aagggcgtag cctttatgtt cggcaaaggt caacagcgaa cccaacggcg actcctgcat
    36421 taccgccgga ttggtcgtcg gcatgccgtt aacgtcaatc aggcaacctg gcggcacggg
    36481 gacgccttta tgccaggcga cgcgggtttt gccaaatgcg atggcgctgg tggcgtaatc
    36541 aagcaacagc gggaaattat ctttacgagg gaaaaccaca cagaacggat tggtgccaaa
    36601 gcggctgtcg cgaccgtgga acggcgcgac cattggaata ccgaccacgc taacaaagtg
    36661 gatagagaca aaccccgccg ctgcacactg ctccgcccag taaccgatac ggccgatatg
    36721 atgcgagtta tgtagcgcca cggcagcaat accgtgctga tgcgctttct caatccccag
    36781 cgccatcgct tcgtgtgccg cgacctgacc aaatgcgcga tcgccatcaa gcgtaaccac
    36841 cgcgcccgct tctttaacga ttttggcatg gtggttaatt tgcagatgcc cctgactcca
    36901 ggaacgcaca tagcttggga tcatgccaat accatgtgaa tcatgccctg ccaggtttgc
    36961 cgcgattaaa tgatcggcaa ctaatttcgc ttcttgttcc tcgctaccca tctgacgaaa
    37021 tacagcctga ataaaactgt gcagcgtctg agcatcaaag cgatgaccac tttccatggt
    37081 ttactcctgt ttatgttatg tgtttgttgt ttttttatgc tgcacgccgg gcgcgggaag
    37141 gtcaataaag atgcaggaat ttcatattgc ggtggggtaa aagccggatg acatggctca
    37201 tccggctgag ataaaaatta aaatgcctga ttaatgcgcg ccaggccgtc gttgatagtt
    37261 gccgcttcac ccgtagccat caggcagcaa gccatctgga ttttcagcga ttcaggaatc
    37321 ggttcgctgc cagcaaggca acgcttaatc cactgcgccg tggtttccgg atcttttgct
    37381 tgtggcagta actcgctacc agcagtgtcc tgtttttcat acagtacccg catcccttca
    37441 cggtcaatga gattgatctg cgggcagcgc tgcggattag catacacttc accttcagta
    37501 ccatgcatta gtagcgcccg cccgccgata tcactaaaga acttcgcgac gcgtccaatg
    37561 tattccggat gcgaaacgct ggaaagacgc agtgcctcac cttcggcaaa tggcgtcgcc
    37621 agtttcgcca gggtatgtgc actgttacgc acgcccatcc gccagcgcat cgccagttgt
    37681 ttttccagcg gcgggcaaaa cgcgccgaca ggcataaaca ccggttgatg ttcgtcgagc
    37741 ttcgcctgcg cctgcccgcc gtgcaacgtt ggcgtaatac ccatcagttc gaaaatggtt
    37801 tctgtcagca cgcgggttgg atcttcgcta accccgtgaa ccaccacagg aaaaccgagt
    37861 ttgtgcagga gaatcgccaa caacggcgtc aggttggcct gtttacgcgc gccgttgtaa
    37921 ctggggatga caatcggcat cggcttgtct gcaggcggcg tcagcttgat ggtgtgattt
    37981 tgcatggctt cgtaaaagcc gagcatctct gcttcccctt cccctttgat acgcagcgca
    38041 atcaatacgc cgcccaactc aaggtcaggg acttcaccat tgagcatatg ggcatacaga
    38101 ccgcgcgcag tatcccggtc taaatcgcgc gcgtggtttt tcccgcgccc gatctcttta
    38161 atgattttgc gatagtccat ttacgactcc ttacctgact cacatcatta acgccgccga
    38221 cggcgtgggg atttcgtttt ttctttcttt ttaactatac cttcagggac ttccggttgc
    38281 tctatcggaa ataccggtaa tgcatccagt agtcgcttgc cataattttt ggtcagcaag
    38341 cgtttgtcgt agataaccac ttccccccag caaccgtggc ttcgaatcag tcgcccaacc
    38401 tgctgaatca ggttaaacga agcgctcggc aggctttgca cctcaaacgg atagcggttg
    38461 aggcttttca gccattcacc ttcggtgatc accaccggac tgtcgatggg cggaaaagcg
    38521 atcttgtgga tatgcacctg actgagcaga tcgcctttca gatcaagacc ttcggcaaag
    38581 gactgtaagc ccaccagcac gctacgctca ccgttggcaa cgcgtttgcg gtgcagttca
    38641 actaagcgat aacgtggctg atcgccctgc accagcaaca tcaggcgtaa atccgtcaca
    38701 tagtcgagaa agcgctgcat cgcacgcccg ctggcaaaca gtaccaacat accgagatgt
    38761 tttttgctct ccacctgctc acggaaaaag gctgccattt cagcaatgtg ctgctcttcg
    38821 ttgtcgatgg aaggctcaaa gcgcatccgg ggaataacaa ttttgccctg ttcgcagtgg
    38881 ttaaagggcg aatccagcgc cacaaagcgg tcgcccgctt tctctttcag accactcatc
    38941 tcctgtaaac gcgaaaaact gttcagcgaa cgtaaggtcg cggaagtgac aataatgtgc
    39001 ggaatactgc gccacagcag cctttccagc tgatcgctga cacgaatacc cacacagtga
    39061 aaccagagat gcagctgccc ttcgcgctct tcccgcgtcg cccatttagt caccggcgca
    39121 ccggaagatt gcgccagaga cgccagccgc cagagtttgc tttgcgcctc gaacatcccc
    39181 aacgcgcgat tcatctgcaa aatcaaccga tgcagacgta caatgtcatg gctaccggtt
    39241 ttctcactta aatcgttaag aaataactcc gccaggccac gcagcatctc ggtgagtttt
    39301 gccagccgct ggcagatctc cagcacttca tcaggcagtt cgcccatcgc aaaacggtgc
    39361 tctgcctcct gcccggcagg catgtagaga ttgagaatgt tgtttaacga ggcgataagc
    39421 tcatacagct cttcacaatg cgcattcaaa cgttcaggga tcgccagcgg tgggatggtc
    39481 ttcgggcgaa actgctccat gcaggtagcg accagtttcg tgaacaagtc cagctgtagc
    39541 cgataccagg gcgcggtgat ttcggcgctc atctccagcg catcccgcgc cacatctggc
    39601 aggtgatgac cttcgtccag caccagcagc agatttttcg ggtcaggtag tacggcttcg
    39661 ctttccatcg ccgccatcac cagcgcatgg tttgccacca ccacttccgc ttcctgaatc
    39721 tcccgacgag caacaaaaaa cgggcattca cgatagtagt aacagttgcg gttgaggcag
    39781 ctggctttgt cggtacttaa acgacgccag agatcgtcat caatggcgat atccgtatga
    39841 tcgcgcagac catcccattt ataagtgtcg agatcgccct tcagcttcgc acaacgtttt
    39901 tgctcttcct gattgttcgg cgtcagctcg tcgtcaagaa acgccagcag atcctgttgc
    39961 gtgggttcag tactggcaag cgctgtcaga ttacgcggac aaacgtagcg cccacgccca
    40021 aaagcagcag tgaatttaag atcgggaatg atctttttca gcagaggcaa atctttactg
    40081 tagatctgat cctgcaatgc cacgttggcg gtactcacca ccagcgtttt ttgctcttcg
    40141 cgggcaatgg cgatgccggg aatcaaatag gagagcgttt tcccaacgcc ggtgggtgct
    40201 tcaatcgcca gatgccgccc ttcttctccg gccagcgttt tggcgacgtc cgcaatcatc
    40261 tgccgctgcg gcgcacgggg aataaagtcg gggatctgtt cctgaagcgc cttataccag
    40321 gcggcaattt gcgctttaag cgcggcggtt aatgccatga gaaaacctga aatactgtat
    40381 aaacagccaa tattgtggca tttttcggtt ttcaggggta atggtttttc tgtttctgga
    40441 aagcggctcg caataaccag caggatgatg cgtaaagcgc ttcgcgctaa attcgaacaa
    40501 catgcagaac tgcgtacact cttgctggca accgcgtccg caaaactggt tgagcatacg
    40561 caaaatgatg cttactgggg agatagcggt aatggtcagg gcaagaatcg cctggggtac
    40621 cttttaatgg cgttgcgcgg acaattggct gcggagaagt aacgcatcgg gtggcttgtg
    40681 ggcgttaaaa cagagaaatc tttttgccgc gctcgcaaat ggctgacaag tgcttgttgt
    40741 gaaatgccgg atgcggcgta aacgccttat ccggcctaca aaagcatgca aattcaatat
    40801 attacagagt tcacgtaggc ctgataagcg cagcgcatca ggcagttttg cgtttgttca
    40861 tcagcctgat gccgggcatc gcccggcatc aaaagattac tgcgcagcgg caggtttacg
    40921 tggacggcgg cgacgttgtt gttcgcctgc cggtttggca tcgccgaggc ggcgagacgg
    40981 tttttctgca ggtttcgcac ttgcagattt tgcgccacct tctccacggc gtggttgctg
    41041 ttgaccgcga ccaccacctt gcccacgacc gccgccacgt tgctggcgac cgttctggat
    41101 cggttcggct ttgattgacg ggtccggctc ataacccgga atcgcaatgc gcgggatctc
    41161 ttttttcagc agtttttcga tatcacgcag cagtttgtgt tcatcaacac acaccagcga
    41221 caacgcttca ccggtagcag ccgcacgacc ggtacgcccg atacggtgga cataatcttc
    41281 cggtacgttt ggcagttcat agttgaccac gtgcggcagc tcttcaatat ccaggccgcg
    41341 cgcagcgatg tcagttgcca ccagtacacg aatatcgccc gatttaaaat cagccagcgc
    41401 acgagtacgc gcaccttgcg atttattacc gtggatcgcc gcactacgga tgccatcttt
    41461 attaagctgt tcagccagat ggttagcgcc atgtttggta cgggtaaaca ccagcacctg
    41521 ctgccagttc cctttcccaa tcatatgcga cagcaattcg cgtttgcgtt tcttatcgac
    41581 aaagtgaacg tgctgggtca cctgatcaga cgcggtattg cggcgtgcca cttcgatttc
    41641 cagcgggttg tgcaacagtt tttccgccag ggctttaata tcgtcagaga aggtcgcgga
    41701 gaacaacagg ttctggcgct tcgctggcag ttttgttaac acgcgacgga tatcgtggat
    41761 aaagcccatg tcgagcatgc ggtcagcttc atcaaggacg aggatttcaa cctgatccag
    41821 cttcactgca ttttgatgtt ccaggtccag cagacgtccc ggcgttgcca ccagcacatc
    41881 aacgccgccg cgcaacttca tcatttgtgg gttaatgctg acaccgccaa acaccaccag
    41941 cgaacgaatg ttcagatatt tgctgtaatc acggacgttt tcgccaatct gcgccgccag
    42001 ttcacgggtc ggggtaagaa tgagcgcgcg taccggacga cgccctttgg cgtgcggctg
    42061 gcgagtgatc aggtgttgca acagcggcag cgtaaagccc gctgttttgc cggtaccggt
    42121 ctgggcgctg gccatcaggt cgcggccttc cagcaccgca gggatcgcct gctgctgaat
    42181 aggggtgggt tcacggtaac cctgctcggc aacggcgcgc aggatatcag ggcttaaacc
    42241 caaagaatcg aaagacataa ctactccgaa ccgccccgac cgttaccggt gtagttttca
    42301 gggagatact gaaaaggaaa atgacaaaaa ccacaatgtc atgagggagc ggagtgtagc
    42361 agtttttgtg acgcagcgca taaattatcc ctgtgactgg cgttgctaaa attcttagcc
    42421 atactggaca actctcatat ggagtcataa tcttaatcaa tcatttgatt aagaatggac
    42481 tgtgcgatga ataatcctgc catgacaatc aagggtgaac aggcgaagaa acagctgatt
    42541 gctgccgcac tggcgcagtt tggtgaatat ggaatgaacg ccaccacccg cgagatagcc
    42601 gcccaggccg ggcagaatat cgctgccatc acctattact tcggttcgaa agaggatttg
    42661 tacctcgcct gcgcccagtg gattgccgat tttattggcg agcagttccg tccacacgcc
    42721 gaagaagccg aacgtttgtt cgcgcaacca cagcctgatc gcgcagctat ccgtgaactg
    42781 atccttcgcg cctgcaggaa catgattaag ctgctcaccc aggatgacac cgtcaacctc
    42841 agcaaattta tctcccgtga gcagctctct cccacggcag cctatcacct ggtgcacgaa
    42901 caggtgatta gtccgctaca cagccacctt acacgcctga ttgccgcctg gaccggctgc
    42961 gacgctagcg atacccgcat gatccttcat acccatgcgt tgattggtga gattctggcg
    43021 tttcgtcttg gcaaagaaac tattctgtta cgtaccggct ggaccgcgtt cgatgaagaa
    43081 aagaccgaac tgatcaacca gacggtgacc tgtcatattg acctgatttt gcaaggatta
    43141 tcgcaaagga gtttgtagtg atgaaaaaac ctgtcgtgat cggactggcg gtagtggtac
    43201 ttgccgccgt ggttgccgga ggctactggt ggtatcaaag ccgccaggat aacggcctga
    43261 cgctgtatgg caacgtggat attcgtacgg tgaatcttag tttccgtgtt ggggggcgcg
    43321 ttgagtcgct ggcggtggac gaaggtgatg cgatcaaagc gggccaggta ctgggcgaac
    43381 tggatcacaa gccgtatgag attgccctga tgcaggcgaa agcgggcgtt tcggtggctc
    43441 aggcgcagta tgacctgatg cttgccgggt atcgagatga agaaatcgct caggccgccg
    43501 cagcggtgaa acaggcgcaa gctgcctatg actatgcgca gaacttctat aaccgccagc
    43561 aaggactgtg gaaaagccgc accatttcgg caaatgacct ggaaaatgcc cgctcctcgc
    43621 gcgaccaggc gcaggccacg ctgaaatcag cgcaggataa attgcgtcag taccgttccg
    43681 gtaaccgcga acaggacatc gcccaggcga aagccagcct cgaacaagcg caggcgcaac
    43741 tggcgcaggc ggagctgaat ttacaggact caacgttgat agccccgtct gatggcacgc
    43801 tgttaacgcg cgcggtggag cctggcacgg tgctcaatga aggtggcaca gtctttaccg
    43861 tctcactgac acgtccggtg tgggtacgtg cttatgttga tgaacgtaat ctcgaccagg
    43921 cccagccggg gcgcaaagtg ctgctttata ccgacggtcg cccggacaaa ccgtatcacg
    43981 ggcagattgg tttcgtttcg cctaccgctg aatttacccc gaaaactgtc gaaacgccgg
    44041 atctgcgtac cgacctcgtc tatcgcctgc gtattgtggt gaccgacgcc gatgatgcct
    44101 tacgccaggg aatgccggtg acggtgcaat tcggtaacga ggcaggacat gaatgatgcc
    44161 gttattacgc tgaacggtct ggaaaaacgc tttccgggca tggacaagcc cgccgtcgcg
    44221 ccgctcgact gtaccattca tgctggttat gtaacggggc tggtggggcc ggatggcgca
    44281 ggtaaaacca cactgatgcg catgctggcg ggattactga aacccgatag cggcagcgcc
    44341 acggtgattg gctttgatcc gatcaaaaac gacggcgcgc tgcacgccgt gctcggctat
    44401 atgccgcaga aattcggcct gtatgaagat ctgacggtga tggagaacct caatctgtac
    44461 gcggatttgc gcagcgtcac cggtgaagcg cggaagcaga cttttgctcg cctgctggaa
    44521 tttacatctc ttggcccgtt taccggacgc ctggcgggca agctctccgg cggaatgaag
    44581 caaaaacttg gcctggcctg taccctggtg ggcgaaccga aagtgttgct gctcgatgaa
    44641 cccggcgtcg gcgttgaccc tatctcacgc cgcgaactgt ggcagatggt gcatgagctg
    44701 gcaggcgaag gaatgttaat tctctggagc acctcgtatc tcgacgaagc cgaacagtgc
    44761 cgtgacgtgt tactgatgaa cgaaggcgag ctgctgtatc agggagaacc gacggccctg
    44821 actcaaacta tggccggacg cagctttctg atgaccagcc cgcacgaggg caaccgcaaa
    44881 ctgttgcaac gggcattgaa actgccgcag gtcagcgacg gcatgattca ggggaaatcg
    44941 gtacgtctga tcctcaaaaa agagaccacg ccagacgata ttcgccatgc cgacgggatg
    45001 ccggaaatca acatcaacga aactacgccg cgttttgaag atgcgtttat tgatttgctg
    45061 ggcggtgccg gaacctcgga atcgccgctg ggcgcaatat tacatacggt agaaggcaca
    45121 cccggcgaga cggtgatcga agcgaaagag ctgaccaaaa agtttggcga ttttgccgcc
    45181 accgatcacg tcaactttgc cgtcaaacgc ggggagattt ttggtttgct ggggccaaac
    45241 ggcgcgggta aatcgaccac ctttaagatg atgtgcggtt tgctggtgcc gacctctggt
    45301 caggcgctgg tgctgggcat ggatctgaaa gagagttccg gtaaagcgcg ccaacatctc
    45361 ggctatatgg cgcaaaaatt ttcgctctat ggcaacctga cggtcgaaca gaatttacgc
    45421 tttttctctg gtgtgtatgg cttacgcggt cgggcgcaga acgaaaaaat ctcccgtatg
    45481 agcgaggcgt tcggcctgaa aagtatcgcc tcccacgcga ccgatgaact gccattaggt
    45541 tttaaacagc ggctggcgct ggcctgttca ctgatgcatg aaccggacat tctgtttctc
    45601 gacgaaccga cgtccggcgt tgatcccctc acccgccgtg aattttggct acatatcaac
    45661 agtatggtag agaaaggcgt cacggtgatg gtcaccaccc actttatgga tgaagcggaa
    45721 tattgcgacc gcatcggcct ggtgtaccgc gggaaattaa tcgccagcgg cacaccggac
    45781 gatttgaaag cgcagtcggc caacgatgag caacccgatc ccacgatgga gcaagccttt
    45841 attcagttga tccacgactg ggataaggag catagcaatg agtaatccga tcctgtcctg
    45901 gcgtcgcgtg cgggcgctgt gcgtcaaaga gacgcggcag atcgttcgcg atccgagtag
    45961 ctggctgatt gcggtagtga tcccactgct actgctgttt atttttggat acggcattaa
    46021 cctcgactcc agcaagctgc gggtcgggat tttactggaa cagcgtagcg aagcggcgct
    46081 ggatttcacc cacaccatga ccggttcgcc ctacatcgac gccaccatca gcgataaccg
    46141 tcaggaactg atcgccaaaa tgcaggcggg gaaaattcgc ggtctggtgg ttattccggt
    46201 ggatttcgcc gaacagatgg agcgcgccaa cgccaccgca ccgattcagg tgatcaccga
    46261 cggcagtgag ccgaataccg ctaactttgt gcaggggtat gtcgaaggga tctggcagat
    46321 ctggcaaatg cagcgagcgg aggataacgg gcagactttt gaaccgctta ttgatgtaca
    46381 aacccgctac tggtttaacc cggcggcaat aagccagcac tttattatcc ccggtgcggt
    46441 gaccattatc atgacggtca tcggcgcgat tctcacctcg ctggtggtgg cgcgagaatg
    46501 ggaacgcggc accatggagg cgttactctc tacagagatt acccgcacgg aactgctgct
    46561 gtgtaaactg atcccttatt actttctcgg gatgctggcg atgttgctgt gtatgctggt
    46621 gtcagtgttt attctcggcg tgccgtatcg cggttcgctg ctgattctgt tttttatctc
    46681 cagcctgttt ttactcagta ccctggggat ggggctgctg atttccacga taacccgcaa
    46741 ccagttcaat gccgctcagg tcgctctgaa cgcggctttt ctgccgtcga ttatgctttc
    46801 cggctttatt tttcaaatcg acagtatgcc cgcggtgatc cgcgcggtga cgtacattat
    46861 tcccgctcgt tatttcgtca gcaccctgca aagcctgttc ctcgccggga atattccagt
    46921 ggtgctggtg gtaaacgtgc tgtttttgat cgcttcggcg gtgatgttta tcggcctgac
    46981 gtggctgaaa accaaacgtc ggctggatta gggagaagag catgtttcat cgcttatgga
    47041 cgttaatccg caaagagttg cagtcgctgc tgcgcgaacc gcaaacccgt gcgattctta
    47101 ttttacccgt gctgattcag gtgatcctgt tcccgttcgc cgccacgctg gaagtgacca
    47161 acgccaccat cgccatctac gatgaagata acggcgaaca ctcggtagaa cttactcaac
    47221 gttttgcccg cgccagcgcc tttacccatg tgctgctgtt gaaaagcccg caggagatcc
    47281 gcccaaccat cgacacacaa aaggcattac tcctggtgcg tttcccggct gacttctcgc
    47341 gcaaactgga taccttccag accgcgccgt tgcagttgat cctcgacggg cgtaactcca
    47401 acagtgcgca aattgccgcc aactacctgc aacagatcgt caaaaattat cagcaggagc
    47461 tgctggaagg aaaaccgaaa cctaacaaca gcgagctggt ggtacgcaac tggtataacc
    47521 cgaatctcga ctacaaatgg tttgtggtgc cgtcgctgat cgccatgatc accactatcg
    47581 gcgtaatgat cgtcacttca ctttctgtcg cccgcgaacg tgaacaaggt acgctcgatc
    47641 agttactggt ctcaccgctc accacctggc agatcttcat cggcaaggcc gtaccggcat
    47701 taattgtcgc caccttccag gccaccattg tgctggcgat tggtatctgg gcgtatcaaa
    47761 tccccttcgc cggatcgctg gcgctgttct actttacgat ggtgatttac ggtttatcgc
    47821 tggtgggatt cggtctgttg atttcatcac tctgttcaac acaacagcag gcgtttatcg
    47881 gcgtgtttgt ctttatgatg cccgccattc tcctttccgg ttacgtttcg ccggtggaaa
    47941 acatgccagt atggctgcaa aacctgacgt ggattaaccc tattcgccac tttacggaca
    48001 ttaccaagca gatttatttg aaagatgcga gtctggatat tgtgtggaat agtttgtggc
    48061 cgctactggt gataacggcc acgacagggt cagcggcgta cgcgatgttt agacgtaagg
    48121 tgatgtaact tcttatcttt cgccagcaaa gacactaccg ccgggccagc aaggattgcc
    48181 agccctgcaa tcgccagcaa aaagttgttt tccagtacgc gggacaacag ccacagcaca
    48241 atcagcgctg cgccaaaata ccaggtcgtg gtcagctctt ccagcacgtc tgccacctca
    48301 cgccagcgtt gctcggggtg gcgctgaaac accagcatca tcactaccgt gacacaatac
    48361 agtgcgcata atcccacgcc gacgtgaacc agagtcttac tcatccagcc caccaccagc
    48421 agcgccacta ccagtaaatg caacataatc tgccagcaac ttagacccgt tgcgacacga
    48481 acacgttgtt gccacttcat ggcttctctc ctgaagaatt tatctctact tactcagagt
    48541 accgaacttt acggaaaatt ccgtaagacc gcaccttttt gtgacgccga ctacactatt
    48601 tcaatgagat aagaggcaaa gacaggaaaa ttatgcccga tcaaacacaa caattttcgt
    48661 tcaaggtgct caccatcaat attcacaaag gctttaccgc gtttaaccga cgcttcattt
    48721 tgccggaact tcgcgacgcc gtgcgtaccg tcagcgccga tattgtttgc ctgcaggaag
    48781 tgatgggcgc gcacgaagtt catccgctgc atgtggaaaa ctggcccgat acctcgcact
    48841 acgagtttct cgccgacacc atgtggagcg atttcgccta cggtcgcaat gccgtatacc
    48901 cggaagggca tcacggcaac gcggttcttt cacgttatcc cattgaacat tatgagaatc
    48961 gcgatgtttc ggtcgatggt gcggaaaagc gcggcgtgct ctactgccgc attgtgccgc
    49021 cgatgaccgg gaaagcgatt catgtgatgt gcgtacatct gggcctgcgt gaggcgcacc
    49081 gtcaggcgca gcttgcgatg ctcgccgaat gggtgaatga gctgccggac ggcgaaccag
    49141 tattggtggc gggtgatttc aacgactggc ggcaaaaagc taatcatccg ttaaaaatgc
    49201 aggccgggct ggatgagata tttacccgcg cccacggacg cccggcgcgc acgtttccgg
    49261 tgcaatttcc tctactacgt ctggacagga tctatgtcaa aaatgccagc gccagcgcgc
    49321 caaccgcgtt gccgctgcgg acatggcgac acctttctga tcatgcccct ttaagtgcgg
    49381 agattcattt atgaaatgta gctggcgcga aggcaataag atccagttgc tggaaaacgg
    49441 cgagcaatat tatcccgcga tgtttaaggc gattggcgag gcgcaagaac gcatcattct
    49501 tgaaacgttt atctggtttg aggatgacgt cggcaaacag ctgcatgcgg cgctactggc
    49561 agcagcgcaa cgcggggtga aagcggaagt cttgctggat ggctacggtt cgccggatct
    49621 cagcgatgag tttgtcaatg aactgacggc agctggcgtg gtatttcgct actacgatcc
    49681 ccgtcctcgc ctttttggta tgcgcaccaa tgtgtttcgc cggatgcatc gcaaaattgt
    49741 ggtgatcgac gcgcgtatcg cctttattgg cgggctgaat tactccgccg agcatatgtc
    49801 cagctacggt ccagaggcta aacaggatta cgcggtacgc cttgaagggc cgattgtcga
    49861 agatattctc cagtttgagc tggaaaacct gcctggacag agcgcggcac gacgctggtg
    49921 gcgacgtcat cacaaagcgg aagagaaccg ccagccggga gaagcgcagg tattgctggt
    49981 ctggcgcgat aacgaagaac atcgcgatga tattgaacgt cactatctga aaatgctcac
    50041 tcaggcgcgg cgagaagtca ttatcgccaa cgcctacttc ttccccggct atcgattttt
    50101 acacgccttg cgtaaagcgg cacggcgcgg ggtgcggatc aaactgatca ttcagggcga
    50161 accggatatg ccgattgtca gagtcggtgc gcgcttgctg tataactatc tggttaaagg
    50221 cggcgttcag gtatttgagt accgccgccg cccgctccac ggcaaagtgg cattgatgga
    50281 cgatcactgg gcgacagtag ggtccagtaa tctcgatccg ctcagtttgt cactgaatct
    50341 cgaagcaaat gtcatcatcc acgatcgtca ttttaaccag acgctgcgcg ataatctgaa
    50401 cggcattatt gccgcagatt gtcagcaggt cgatgagacc atgctgccga aacgcacctg
    50461 gtggaacctg accaaaagcg tgctggcgtt ccacttttta cgccacttcc cggcgctggt
    50521 tggctggctt ccggcacata caccacgtct ggcgcaggtt gatccccccg cacaaccgac
    50581 aatggaaacg caggatcggg tagaaactga aaacacgggg gtaaaaccct gatgagtaaa
    50641 tcacacccgc gctggcgctt agcaaagaag atcctcacct ggctgttttt tatcgcggtg
    50701 attgtgttgc tggtggtcta cgccaaaaaa gtggactggg aagaggtctg gaaggtcatc
    50761 cgcgactaca atcgcgttgc gctgctcagt gcggtcgggc tggtggtcgt cagctatctg
    50821 atttacggct gctatgacct gctcgcccgt ttctactgcg gtcacaaact ggcgaagcgc
    50881 caggtgatgc tggtgtcgtt tatctgctac gccttcaacc tgacgctcag tacctgggtc
    50941 ggcggcatag gtatgcgcta tcgtttgtac tcgcggctag ggttaccggg cagcactatt
    51001 acgcggattt tctcgctcag tattaccacc aactggctgg gctatatttt gctggccggg
    51061 attatcttta ccgcaggcgt ggtggagctg ccggatcact ggtatgtcga tcaaaccacg
    51121 ctgcgtattc tcggcattgg cttattgatg attatcgcgg tttatttgtg gttttgcgct
    51181 ttcgcgaagc accgccatat gaccatcaaa gggcaaaagc tggtgctgcc ttcatggaaa
    51241 ttcgccctcg cccagatgct gatttccagc gttaactgga tggtcatggg agcgataatc
    51301 tggctgttac ttggtcaaag cgtgaactat ttctttgtac tgggcgtgtt gctggttagt
    51361 agtattgccg gggtgattgt gcatattccg gcggggattg gtgtgctgga agcggtgttt
    51421 atcgcactac tggctgggga gcatacctcc aaaggtagca ttatcgccgc cctactcgct
    51481 taccgtgtgc tgtattactt tatcccactg ctgctggcgc tgatctgtta cctgctactg
    51541 gaaagccagg cgaagaagct gcgggcgaaa aatgaagcgg cgatgtgaag ttcggttaag
    51601 ttctttagta tgtgcattta cgactaatga aaaaaccgcg tatgcctttg ccagacaagc
    51661 gttatagcta tcgcggcaag tatcccaaag aagccgataa aatagtaata gagtgttaat
    51721 gcacctgtaa aagcgcaact aaccggactc cacaactctt cgctatggca ttttcctgcc
    51781 agttcaagga gcttatttcg tccatgcgag ataatgcctg accataaaac aatcattaag
    51841 caacttatta cccaaatggg ccgttcactc attaatgcgg cattcctgat gatcaccagg
    51901 gccagtcctg cggttgtcac caaacccaaa gcggtaatga gccatatgcc gtttaatatc
    51961 ctggcttcgt gctgaaaatc taatttattg ctgctttgcg agtatgattc cattctttta
    52021 gttatcctta gaaaaaatat taatattcac catcaataaa tttaaagcca cgcatacaaa
    52081 atacttttgt atttatggat acggaaaaca cttccgctcg gccataaggc atattttata
    52141 cttggtactt gttaagtgtc gttttttcgt aagaaaaaaa tcccgacaca tggccgggat
    52201 attgatggtg aaaaagaatt aacggcggtt gccgaagatc cgcaacaaca tcaggaacaa
    52261 gttgatgaag tccagataca aggttaacgc gccaagaatg gaatatttgc gcaggttcga
    52321 cgtgtcgcgg gtatcaatct gctcacccat atttttcaat ttctgcgtgt catacgccgt
    52381 caatccgaca aagacaatca cgccaatgta ggtaactgcc cacatcaatg cttcgctttt
    52441 cagccagaag ttgaccagcg atgccagcac aatgccgatt aacgccataa acagcatatt
    52501 gccaaagccg cttagatcgc gcttcgtggt gtaaccgtac aggctcattg cgccaaacat
    52561 tccggcagta acgacgaaag tactggcgat agaagcagcg gtataaacaa tgaaaatact
    52621 ggaaagcgta agacccgtca gcgccgaata aagcataaag agcatcgtcg ttacacctgc
    52681 gctcagcttt tgaatcatcg ctgataacac aataaccaat gctaattgcg cgatgatcag
    52741 accgattaaa aagacacggt tagtgaacaa cagctccatc acagccgcgg aattagccgc
    52801 ataccaggca acaaatgcgg tcagcaacaa gccaacggtc atccagccat agacttgcgc
    52861 catataagtt tgcaagccag cccggggttg tacaattgaa tcagaacgtg ggaatctgtc
    52921 catgacgatc tcctgaagat ataaggaata tcttaaggat actgcaaaat gatgaggctg
    52981 tgcatcgacg cagcgtaaac gcatgtactg agcggtgaaa ttgccggacg cagcggtgcc
    53041 ttatccggct aacaaaaaac taccagcgtt ttgccgcctg ctgatcgctc tcccgagctt
    53101 caacccagcg gtcgccttcc ggcgtggctt cacgcttcca gaacggtgcg cgggttttga
    53161 gataatccat aataaactgc ccggcttcaa acgcactgct gcgatgcgca ctggtgacac
    53221 cgacaaaaac gatttcatct cccggccata attccccgat gcggtgaatc accgtgacgc
    53281 gccccagcgg ccagcggtta cgcgcttcat cgacaatttc tgccagtgct ttttcagtca
    53341 tccccggata gtgttcgagg gttaatgctt tgacgctgtc gccaaggtta tggttgcgca
    53401 ccttaccggt aaaggtgact accgcaccgt cttcatcacg ctccgccagc cacggatact
    53461 cttctcctac gctgaatggc tgcgggccga caacaatttt ggtttctgcc atcttaacct
    53521 ccggttaccg gcgggaagaa agccacttcg tcgccgtcag tcagcggatg gtcaagactc
    53581 accagcgtct ggttgacggc agccagcaat ttgccatctt ccagcgccag cgcccagcga
    53641 tcgctctgcg cagccagatg ttggcgtaac gcttcaacgg ttgggaaatc cgcagccact
    53701 tcggttgcat ctgttcccac caactcgcgc acctgggcga aaaaaagaac tttaatcatc
    53761 cgcttccacc ttaaagtcac ccgacttgcc gccgctcttc gccagcaaac gtaccggacc
    53821 aattaccata tctttttgca ccgctttgca catgtcataa atggtcagcg ccgccacgga
    53881 ggccgcggtt aacgcttcca tttcgacacc ggttttcccg gtcaggcggc ataaggtttc
    53941 tatacgtacc cgattgtgct ccggctcggc ctgtaaattg acttcaactt tgctgagcat
    54001 cagcgggtga cacagcggga tcagatccca ggtgcgtttt gccgcctgaa taccggcgat
    54061 acgcgcagtg gcaaatacgt cgcctttgtg gtggcgacca tcaataatca tcgccagcgt
    54121 ctcgctgcgc atggtgacaa aggcttccgc ccgcgcttca cgcacggttt ccgctttggc
    54181 ggagacatcc accatgtgtg cttcgccagc ggcgttgata tgggtcagtt gcgacatact
    54241 tatttcttca aatgtggatg gaaattgcat ggacgtgtac gggcatcgag ctgcggcgcg
    54301 atgatatttt cccacgcggt gcggcacgct ttggtcgaac ccggcatggc gaaaatcagc
    54361 gttttgttag cgacgcccgc taccgcacga gattgcaacg ttgaggtgcc aatctcttca
    54421 aacgacaaca tccggaacac ttcaccaaaa ccttcaactt cacggtcgaa caacggcagc
    54481 agcgcttcgg gagcctgatc accttccgtc aggccagtac cacccgtaat caataccact
    54541 tgtacatcgt cgctggcgat ccacgcagat acctgagcgc gaatagcgta gcggttttct
    54601 ttcacaatgg ctttatcgac aacgtgatgg cccgcttcct gcgccgaatc gcgcagatag
    54661 tgaccggagg tatcgtcttc ttcaccacga cgattagaaa ccgtaagaat agcaatacgg
    54721 gtcgggataa attcagtgct tacctgactc atctgatctc tccttttgac gttttagccg
    54781 ccaatgtacg ataagttttg cgtaataccg gtgttgtttt gatgcaggaa atgggtctgt
    54841 ttcttctccc gcagcgccgc tgaaatacgc gcttccagcg cctgttgctg ggtatcgtct
    54901 tccagcagat cgcgcaggtt aacgccgcct tcaccaaaca ggcagagatg gagtttacca
    54961 atggaggaaa cgcgcaggcg gttgcaagtg gcgcagaagt ctttttcata cggcatgata
    55021 aggccaatct ctccggcgta atccggatga caaaagactt gcgcgggacc gtcgctgcgt
    55081 tgacgtaatt ggtggatcca gccgcgacgc agtagctcgt cacgcagaac ctgaccagag
    55141 atgtgatgct tacggaagag ctcgctgccc tcacccgttt ccatcaattc gatgaaacgc
    55201 agctggatag ggcgatgctg gatccagttc agaaaggtgt cgagctgatg atgattaaca
    55261 tcacgcatca gcacggtatt gactttgacc ttctcaaaac cggcctcaaa tgcagcatcg
    55321 atccctgcca tgacctggtt gaatttatcc tgcccggtaa tggcatgaaa ctggcgagcg
    55381 tcaaggctat caacgctgac gttgatgcca gtaagtcccg catcgcgcca gttcgccaca
    55441 tcgcgttcca gacggtaacc attggtggtg accgcaatct ggcggatagc gtcgttttcc
    55501 cgcacagcgg cgatgatatc ggtaaagtcg cggcgtaaag acggctcacc gcctgtcagg
    55561 cgcacttttt cggtgcccag actggcaaag gcgcgcgtaa cccggcgaat ttcatcgacg
    55621 gtaagaaagc ctttattggt gacgccgctc ggtttgtagc catccggcag gcagtaggtg
    55681 caacgaaagt tacacacatc agtaatcgac aggcgcaagt agtaaaactt acgcgcaaat
    55741 gcatcagtca gttgtgaagc catgtacacc tttccagata cgggaggcga agtcatttct
    55801 tccttcgccc tggtggcaat ctttacgagc attgtcacgg ccaaagcacc gtatcagttg
    55861 acccaggtgc agaggctaga gtgtttagtg gttaagccga tactagcgtg taaatgagat
    55921 ttttaccatc cacactttcg ctatgtaatc atgtatatag cgtcatgatc gtgctatttc
    55981 gctacagaat aagggaaaat ggcggtttta ctttgatata catcatcagg aaggcatagc
    56041 gtacatcgtc ctttagaggt agaagaaaat agtttgtatc ctcaatattg gcaggttaat
    56101 tgctgtttcc ccgcaatttg cgctactgta gcgcggataa tctgactcca ggaaactata
    56161 tgcgcaatcg tacgctggct gaccttgatc gtgtcgttgc tctcggcgga gggcatggac
    56221 tgggacgcgt tctctcatca ctttcgtctt tgggttctcg tttaacgggt atcgtcacca
    56281 ccacggataa tggcggatcg acagggcgta ttcgccgctc agaaggcggt attgcctggg
    56341 gcgatatgcg taactgcctc aaccagctga taactgaacc cagcgtcgct tccgcaatgt
    56401 ttgaatatcg tttcggcggt aatggcgaac tttccgggca caaccttgga aatctgatgc
    56461 tgaaggcact ggatcacctt agcgtgcgac ctcttgaagc aatcaatctg attcgcaatc
    56521 tgctgaaagt ggacgcacat ttgattccga tgtcggagca tccggtggat ctgatggcga
    56581 ttgacgatca ggggcatgaa gtttacggcg aggtcaatat cgaccagtta actacgccga
    56641 ttcaggagtt actgttaacg ccgaatgtac ccgcaacgcg tgaggctgtt cacgctatca
    56701 gtgaagcgga tctcatcatt attgggcctg gcagttttta caccagcctg atgccaattc
    56761 tgctgctgaa ggaaatcgca caggcattac gccgcacgcc agcgccgatg gtctatatag
    56821 gcaatctggg gcgtgagttg agtttacctg cggctaattt gaagctggaa agcaagctgg
    56881 caattatgga gcagtatgtc ggtaaaaagg tcattgatgc ggtcatcgtc gggccaaaag
    56941 tggatgtctc ggcggtgaaa gagcggattg tgattcagga ggtactggag gccagcgata
    57001 ttccgtatcg tcatgaccgc cagttgttac atagcgcgct ggaaaaggca ttacaggctt
    57061 taggttaacg gaaagccgaa ggaaaattcc ggcttcctat tgaagacaaa gtgcgcgttg
    57121 tttatgccgg atgcggcgtg aaagccttat ccggcctaca aaccgcgcta attcaatgta
    57181 ttgcagagat catgtaggcc tgataagcgt agcacatcag gcaattttgc ttttgtcatc
    57241 tgtcttcttc gttatcctgt cacgaagccg caatgaacaa ctcacgcagc tgatgcaact
    57301 ggtcacgaat ttgtgccgct tcttcgaact ccagattctg cgcgtgttgc atcatcaacc
    57361 cttccagttc atggattttc tgctgcaacg ctttaggcga catatccatc ggcacattat
    57421 ccggctcaac aatcggtcgc gattttcctc tgcccttcgc tttggttttg gcaatgttct
    57481 gccccagcgc caggatatcg accactttct tgttcaagcc ttgcggcgta atgccgtgtt
    57541 cctcgttgta cttctgctgt ttctcgcggc gacgttcggt ttcgccaatc gctttcgcca
    57601 ttgatggggt gatcttatcg ccgtagagaa tcgctttacc gttaacgtta cgtgccgcac
    57661 gaccaatggt ctggatcaac gaacgttcgg aacgcaggaa gccttctttg tcagcgtcga
    57721 ggatcgccac cagcgaaact tccggcatat ccagaccttc gcgcagtaag ttgatcccta
    57781 ccaatacgtc gaactcaccc agacgcaagt cgcggataat ctccatacgt tcgacggtgt
    57841 cgatatctga gtgaagataa cgcacgcgct caccgtgttc ttcgagatat tcagtgagat
    57901 cttccgccat ccgcttggtc agagttgtaa ccagtacgcg ttcgttaatt gccgctcgct
    57961 gacgaatctc cgaaagaaga tcatccacct gtgttgccac tggccgcact tcgataatcg
    58021 ggtcgagtaa gcctgttgga cgcaccacct gatccaccac atcgccaccg gatttttcca
    58081 gctcgtaatt acccggcgtc gccgaaacat agatggtttg cggcgctaat gcttcgaact
    58141 cttcaaattt cagcggacgg ttatccagcg ctgatggcag gcggaagccg tactccacca
    58201 gtgtctcttt acgcgcccgg tcaccgcgat acatgccgcc aatttgtgga atggtgacgt
    58261 gagattcatc gaccaccagc agcccatcag caggcaggta atcaaacagc gtcggcggtg
    58321 gctcacccgg tccacgaccg gagaggaagc gcgagtagtt ctcaatcccc gaacagtagc
    58381 ctagctcgtt catcatctcc agatcaaact gggtacgctg ggtcagccgc tgctcttcca
    58441 gcagtttgtt gttttccaat agcactttac gtctggcggc cagctcttct ttgatctcct
    58501 ccatcgcctg gacgatgcgc tcgcgtggcg tgacgtagtg cgttttcggg tagatggtaa
    58561 aacgtggaat agtggaaaca atctgcccgg tcagcgggtc aaataacgac aatcgttcca
    58621 cttcctcgtc aaacagctcc acgcgaagtg caatgtcatc cgattctgcc gggaagatat
    58681 caatcacctc gccacgaaca cggaaagtac cacgctggaa ggcttgatca ttacgagcat
    58741 attgcagctc cgccagtcgg cgcaggatcg cgcgctgatc gataatcata ccgaccgtga
    58801 gatggagcat catcttgaga tataaatcag gatcgcccag accataaatc gcggaaacag
    58861 acgccaccac aaccacatca cgccgctcca gcatcgcttt ggtggcggac aaacgcatct
    58921 gttcgatatg ttcgtttacc gaggcatctt tctcaatgaa ggtgtcggaa ctcggtacat
    58981 aggcctccgg ctgatagtag tcgtagtagg agacgaaata ttccaccgcg ttttccggga
    59041 agaattcttt catttcgcca tacagctggg ccgccagcgt tttgttgggc gcaagtacca
    59101 tggttgggcg ctgaaggtca gcaatgacat tggcaatggt gaaggttttc ccggagccag
    59161 tcacgccaag taacgtctgg tgcgccaggc catcttccag cccctcttcg agacgtcgaa
    59221 tcgcctccgg ctgatcgcca gaaggtttaa aagcggaatt cagtttgaac ggtttactca
    59281 tgagtcgcta cctgaaggag ttgggcgggc aggtatgtaa ttttactcgt cgtacttaat
    59341 tatgccaaca aattatactg gataaaaaaa cagttcatca ccataatatt tctgttacag
    59401 cgtaaactcc gctgtcaatc atgagcaaaa tttactctgc ggcgagataa aactccggcc
    59461 ttaccgggtt atccccaaag caacggcttt tttaacattt gtcaagatga ttgatgacag
    59521 ttttatggca agagatgcct gttcagtgac tgccattgca attatctaac ctgttaaaaa
    59581 ataaaagata tttctttgag ccgtctttaa cgccaattcg cgtgcaagcc gcgtattctc
    59641 tcgcttgcct cgtgttttct aactctaata cacatggtta tccacaggaa tagtggataa
    59701 ctgtctgcag cccctatccc ccgccgcctg ggcaattccc acattcaccg catgatgcgg
    59761 aggtaaaatt tttttcgtga tttttttaga atttaattgg ttaaattgca gtcaatcgga
    59821 gacacgatct cgctcgcaat ttaaccaatt acaggatgac tacattaagg caagattgat
    59881 gtactttccg gttgccgcat tttctggatt ttctgcaagc cagggaattt cgcccagcag
    59941 cggcgcggga atcatgcggg tgagcgtggt catatattca gcgtgacgtt ttcccggagg
    60001 cgtgacatcg ttcgccaccc accccgccag agtcagtccg gcgtgtagta ttgcctgcgc
    60061 agtcaacatc gcgtgattaa tacagccgag tttcacacca actaccagta tcaccggcag
    60121 ttgttcctgt gttacccaat ccgcaaaagt gaaagtgtca gaaagcggcg taaaccagcc
    60181 gccagcacct tccactaaca cccagtcagc ctgttgttca agcgcgcgta atccggcgct
    60241 cattaccaat gattctatcg gcctgccctc ttgcgcgctg atgatatgcg gcgaggtggg
    60301 ttctgcgaag gtgtaaggat ttaccgttgc gtaatccagc tgcaggctac tgttgcgctg
    60361 taacgccagc gcgtcactat tacgtaagcc tttcggggtc ttttcgctgc cagaggccac
    60421 cggtttataa cctgccgtcc ggtagcctgc tgcctttgcg gcttgtaaaa gcgcgcagct
    60481 ggcgacagtt ttccccactt cggtatccgt tccggtgaca aaataacatt tactcacgag
    60541 caatcactcc caaaaaaaga tgatacgtca gaggatatcg cccctgttgt tgcggccagg
    60601 ctagttgcaa tcgctgcaac tgcgaacgcg ttaatattcg cgggtcgcgc ccttcatgaa
    60661 gatgcgtggc accgatgcct ttcagcgaac gcatggcact gagcgcatca tcaaaccaca
    60721 gcgtaatggg ctgaatataa tgttgataat gcacggcgtt tagcgactgt tcgattttat
    60781 ctggcggtaa aaagcgatta gcatgcgggc gctcgtccac cgcctgccac gcctgatgca
    60841 gttcgggtaa cgatccctgc accagcgtgg taaacgcgac cacgcctccg gggcgcacca
    60901 cccgatacag ctcgcggagt gccatggata aattaccgca ccactgcacc gcgagattgc
    60961 tccatgcaag atcgaacgtt gcagtcgcta acggcaggga ttcgatatct cccgccagat
    61021 aatggtctgc ggcatccttc tggcgtgcct gaacaagcat tggcggagag agatctaacg
    61081 cagtcacctg cgcgtgacgc tctcgccagc ggcggctcat ccagcctggt ccacaacccg
    61141 catccagtac gtgggtgtat ttacgctgtg ggagcattgc cagtaaggcg tcagcactct
    61201 ggcgctgtag atcggcgtgt tgctcatagt gtgcggctgc ccgtccaaat gccgctgcaa
    61261 tggcttgttt attaaccgtt gccatgcagc acctccagca gacggtcgat atcctgcatt
    61321 tcatgcgcgg ctgttagtgt taagcgcagt cgcgcagtac cagcaggtac ggttggcggg
    61381 cgaatcgccg tgacccagca gccttgctgg cgcagttttt ctgccagttg taacgcacgg
    61441 ctgttatcac caacaatcaa tggctggatg gcgctgcatg aatcagcaag cgtaaacggc
    61501 aaatcctgta ctccggcacg aaaacgcgta atgagtgccg ccagtttttc gcgccgtgta
    61561 tcaccctcat cactgcgaat gaccgccagc gatgcacgta atgcctgcgc ctgagcgggc
    61621 ggcatactgg tgctgtaaat aagatggcgg gcaaattgca ccagataatc cgccaccgta
    61681 tcggagcaaa gcaccgctgc cccgctgacg ccaaatcctt tgccaaaagt caccaccagc
    61741 aattctggtt ttaccttttg cagccagcag ctgccgcgcc cctgctcccc gataacgccc
    61801 gtgccgtggg cgtcatcgac catcaaccag ccatcgtgct gttgcgttac ctgctggatt
    61861 tcctccagtg gcgcactatc gccgtccatg ctgaacacac cttctgtcac taccagttgc
    61921 tgccccggac agggggaagc aagcagtcgc gccagatgag tgacatcgtt atgagcaaaa
    61981 cggcgaagcg gcgacgggct taaacttgca gcctccagca atgaggcatg gctaagccgg
    62041 tcggcaacaa tacggtcctc tttcgccatc atcgcggcaa tgactgcctg attagccgca
    62101 aaaccggaga taaacagcag cgcccgcgaa tagcccagcc actcggccag ttcttcttcc
    62161 agcgcctgat gtgccacgct ataaccgctg acgtgaccgg agccgccgct accgacgcca
    62221 aattgctccg cactctgctt ccaggcacgg ataatttgcg gatgatggct taaacctaaa
    62281 taatcgttac tggaaaagtt cagatactgg cgatcgtccg ccaccagcca gcgtccggcc
    62341 ccttgcgcca ccggataacg gcgacgcagg gcatcggcag cacgccgcgc atcgagcgcc
    62401 gcgttgattt tctcctgcca gctcatagtg ctgccgcgtt gtaatattcg tcagtgtccg
    62461 gggtcatcag tgcctgctcc agacgctgct gttgttcgtt atcccctgcc agcacggcag
    62521 tttgctgtgg atttagcccc agtttgcgga acagttgcag gtctttatct tcttccggat
    62581 tcggcgtggt tagcagtttg caaccgtaga aaatcgagtt agccccggcc ataaagcaca
    62641 tcgcctgagt ctgttcattc atctgctcgc gtccggcaga aaggcgcacg tatgaggtcg
    62701 gcatcatgat ccgcgctacg gcaatggtgc gaataaaatc aaaggcatcg acatcatcgt
    62761 tatctgccag tggagtacct ttcaccttca cgagcatgtt gattggcacg ctttccggcg
    62821 gcgtcggcag gtttgccagt tgcagtaata atccggcacg atcttttacc gtttcgccta
    62881 aacccacaat gccacctgag cagactttga tcccggcatc gcgcactttt tccagcgtat
    62941 caaggcgttc ctgataagta cgtgtggtga tgatattgcc gtaaaactcc ggcgaggtgt
    63001 cgaggttatg gttgtagtaa tccagcccgg cattcgcaag gcgctgcgcc tgagattcac
    63061 tcaacgtacc cagcgtcata cacgcctcca gccccatcgc ttttactccc tgcaccattt
    63121 gttccaggta gggcatatcg cgttcgtggg gattcttcca cgccgcgccc atgcagaagc
    63181 gcgtcgatcc tgccgctttc gctttgcgcg ccgacgccag cacctgttca acttccatca
    63241 accgctcggc ttccagcccg gttttgtagc gcgagctttg cgggcagtat ttgcaatctt
    63301 ccggacaagc tccggtctta attgacagca aagtgctgac ctgcacctga cgtggatcga
    63361 aatgctgacg atgcacctgc tgcgcttcaa acagcagatc cagcaacggt ttttcaaata
    63421 attctgtgac ttgcgacaat gtccagcgtg ggcggtgagc catggggctt ctccaaaacg
    63481 tgttttttgt tgttaattcg gtgtagactc gtaaacctaa atcttttcaa tttggtttac
    63541 aagtcgatta tgacaacgga cgatcttgcc tttgaccaac gccatatctg gcacccatac
    63601 acatccatga cctccccgct accggtttat ccggtggcga gcgccgaagg ttgcgagcta
    63661 attttgtctg acggcagacg cctggttgac ggtatgtcgt cctggtgggc ggcgatccac
    63721 ggctacaatc acccgcagct taatgcggcg atgaagacgc aaattgatgc catgtcgcat
    63781 gtgatgtttg gtggtatcac tcatgcgcca gccattgagc tgtgccgcaa actggtggcg
    63841 atgacgccgc aaccgctgga atgcgttttt ctcgcggact ccggttccgt agcggtggaa
    63901 gtggcgatga aaatggcgtt gcagtactgg caggccaaag gcgaagcgcg ccagcgtttt
    63961 ctgaccttcc gcaatggtta tcatggcgat acctttggcg cgatgtctgt gtgcgatccg
    64021 gacaactcaa tgcacagcct gtggaaaggc tatctgccag aaaacttgtt tgctcccgcc
    64081 ccgcaaagcc gcatggatgg tgaatgggat gaacgcgata tggtgggctt tgcccgcctg
    64141 atggcggcac atcgtcatga aatcgccgcc gtgatcattg aaccgattgt ccagggcgca
    64201 ggcgggatgc gcatctacca tccggaatgg ttaaaacgaa tccgcaaaat gtgcgatcgc
    64261 gaaggtatct tgctgattgc tgacgagatc gccaccggat ttggtcgtac cggcaaactg
    64321 tttgcctgtg aacatgcaga tatcgcgccg gatattttgt gtctcggtaa agccttaacc
    64381 ggcggcacaa tgaccctttc cgccacgctc accacgcgcg aggttgcgga aaccatcagt
    64441 aacggcgaag ccggctgctt tatgcatggg ccaactttta tgggcaatcc gctggcctgc
    64501 gcagcagcaa acgccagcct ggcgattctc gaatctggcg actggcagca tcaggtggcg
    64561 gctattgaag ggcagctacg cgagcaactt gcccccgcct gtgacgccga aatggttgcc
    64621 gatgtgcgcg tactgggggc aatcggcgtg gtcgaaacca ctcgtccggt gaatatggca
    64681 gcgctgcaaa aattctttgt cgaacagggt gtctggatcc ggccttttgg caaactgatt
    64741 tacctgatgc cgccctatat tattctcccg caacagttgc agcgtctgac cgtagcggtt
    64801 aaccgtgcgg tgcaggatga aacatttttt tgccaataac cgggagtcag cgtgaggggt
    64861 tctggctaca ctttctgcaa actagaaagg agggttcatg aaactcatca gtaatgatct
    64921 gcgcgatggc gataagttgc cacatcgtca tgtctttaac ggcatgggtt acgatggcga
    64981 taatatttca ccgcatctgg cgtgggatga tgttcctgcg ggaacgaaaa gttttgttgt
    65041 cacctgctac gacccggatg cgccaaccgg ctccggctgg tggcactggg tagttgttaa
    65101 cttacccgct gatacccgcg tattaccgca agggtttggc tctggtctgg tagcaatgcc
    65161 agacggcgtt ttgcagacgc gtaccgactt tggtaaaacc gggtacgatg gtgcagcgcc
    65221 gccgaaaggc gaaacccatc gctacatttt taccgttcat gcgctggatg tagaacgtat
    65281 tgatgtcgat gaaggtgcca gcggcgcgat ggtcgggttt aacatccatt tccactctct
    65341 ggcaagcgca tcgattaccg cgatgttcag ttaattactc tgccagatgg cgcaaagcca
    65401 tctggtatca cttaaaggta ttaaaaacaa ctttttgtct ttttaccttc ccgtttcgct
    65461 aaagttagta taaaaaagca ggcctcaacg gattcatttt tctatttcat agcccggagc
    65521 aacctgtgaa cacattttca gtttcccgtc tggcgctggc attggctttt ggcgtgacgc
    65581 tgaccgcctg tagctcaaca ccgcccgatc aacgtccttc tgatcaaacc gcgcctggta
    65641 cctcttctcg cccgattctg tcggcaaaag aagcgcagaa tttcgatgct caacactatt
    65701 ttgcatccct gacaccaggt gcggcagcgt ggaatccttc cccgattacc ctgcctgcac
    65761 aacctgactt tgttgtcggc ccggcgggta ctcaaggtgt aacgcatacc acgattcagg
    65821 cggcggtaga tgcggcaatt atcaagcgta ccaacaagcg ccagtatatt gccgtgatgc
    65881 ctggtgagta tcagggaacg gtatatgtcc ctgccgctcc gggtggaatt actctgtacg
    65941 gtacaggtga aaaaccgatt gatgtgaaga ttgggctttc ccttgatggg ggcatgagcc
    66001 ccgccgactg gcgtcacgac gtcaacccgc gcggcaaata tatgccaggt aaaccagcgt
    66061 ggtatatgta cgatagctgc cagagcaaac gcagcgacag tatcggcgtt ctctgctctg
    66121 cggtcttctg gtcacaaaac aatggactgc aactgcaaaa cctgaccatc gaaaacacgc
    66181 tgggcgatag cgtagatgcg ggtaaccatc cggcggtggc actgcgtact gatggtgacc
    66241 aggtacagat taacaacgtt aacattctcg gtcgtcagaa caccttcttt gtcaccaaca
    66301 gcggtgtgca gaaccgtctg gaaacgaatc gtcagccgcg tacgctggtg accaacagct
    66361 acattgaagg ggatgtggat atcgtttctg gtcgcggcgc agtggtgttc gataataccg
    66421 aattccgcgt ggtgaactca cgtactcagc aagaagcgta tgtgtttgca ccggctacgc
    66481 tgtccaacat ttactacggt ttcctcgccg taaacagtcg tttcaatgct tccggtgatg
    66541 gcgtggcgca actgggccgc tcgctggatg ttgatgccaa taccaacggt caggtggtga
    66601 tccgtgatag cgccatcaac gaaggtttta acacagccaa accgtgggct gatgcggtga
    66661 tttccaatcg tccattcgcg ggtaacaccg gcagcgttga tgataacgac gaagtacagc
    66721 gcaatctgaa tgacactaac tacaaccgca tgtgggaata caataaccgc ggcgtgggta
    66781 gcaaagtggt tgcagaggcg aagaagtagg agctgttaac tatgtgccgg atgcggcgta
    66841 aacgccttat ccggcctacg gttcgatgcg atttgtaggt cggataagat gcgccagcat
    66901 cgcatccgac aataagtgcc ggatgcgacc tacattcaca tggcgctttt tacatctgac
    66961 ggtttttatt gaagttaatc aaactacccg ccttgataat ctcgcgctct tcagcagtca
    67021 gactttccat atagagcgta atctccgtta ccggtgcatc ttcatggatc acataacctt
    67081 taaacgtcgt tccaggatta tccagcgccg ctttaatgcc agggatgtaa atgtaatccc
    67141 ccacttcaaa cgttggtgct tccgccattt gcagcggtaa catcccccag ttgatgacgt
    67201 tagaacggta gcgtttagtc gcgtattcct cggcaatatt cgccagaccg ccaatcacac
    67261 gctggcagct cgccgcctgc tcacgcgcag aaccatcgcc tggtttcacc gcatagacca
    67321 tgctgccaat ttcagtttgc agcggatcaa tatgctcctg accagcaatc tgcttaatgc
    67381 gcgcaaacac ctctgtcagc tcgctgacat tccccgccag acgctgattt tccagctcag
    67441 cagtcgcttt acttctgcca acataaccgg gatcgcggcg tgacagggta aactccgcca
    67501 gaccaatcgg atttgaacga taagaagagg tttcaccgga aggaatcagt tcgtcggtgg
    67561 tggtgacttc gtcgaggatc ttcgagcaca ctttgaggac gatattgtca gtcagcgcgc
    67621 ccaattccgg ccagtctttg atgttcggtc cgtagatcag cggttgctgg gtcgcccctt
    67681 tcacaaagcc ttgatagacg cgatttttat acggcgttac atcgaaggcg tactccggca
    67741 cgttgtccca gcaatcgagt tcgctggcag aggttaaata gccaccgttt gccgcagttg
    67801 cagcgataga acgagcgtcc atcaacgcca ccgctgacat ctgcccatta gctggcttag
    67861 agccttcgcg gttcgggaag ttacgcgtgg tgtggcgaat actcaaaccg ttgttgattg
    67921 gcgtatcgcc cgcaccaaag catgggccgc agaacgcggt tctgatgatt gcgcctgcgc
    67981 caatcaaatc tgctaccaca ccttttttgg cgagatccat aaacaccggc tgtgatgacg
    68041 ggtaaaccgc caatgagaag gtatcgttgc cgcaggattg accgcgcagc gcattcgccg
    68101 ccgcgatgac gttttcgtag ttaccgccag aacagcccgc gataatcccc tgctgcactt
    68161 tcaggcgacc attttccact ttatccagca gcgagagttt ggctttaccg tgcgccacgc
    68221 gttcggactc aatttcaatc tcacgcagaa tgtcggtcag gttctggttc agcgtgtcga
    68281 tttcatacac gttgctcggg tggaatggca gcgcaatcat tggtttgatg gcgcttaaat
    68341 caacgctgat gcagccatcg tagtacgcca ttggttgagg gttaagttgg cagtaatcct
    68401 ggccgcgacc gtgcagcgcc agccagttat ggacttcttc atcggtttgc cagacagaac
    68461 ttaaacaggt cgtttcagtg gtcatcacgt caacgctgtt acggaaatcg gtagagagcg
    68521 ctgcaacacc gggacctacg aactccatca ctttgttttt gacgtaaccg tttttgaaca
    68581 cggcaccgat gatagccagc gccacatcct gcggccccac atacggcgca ggttttccgg
    68641 tcagatgcac cgcaaccacg cccggatagt cgatatccca ggtgtcatta agcagctgtt
    68701 ttaccaactc gccgccgccc tcgccaaccg ccattgtccc taacgcaccg taacgggtat
    68761 ggctgtctga cccgaggatc attttgccgc cgcccgccat catttcacgc atatattgat
    68821 ggatgaccgc aatatgcggt ggcacaaaaa taccgccata acgctgggcc gccgataaac
    68881 caaaaacatg gtcatcaccg ttaatggtgc cgcccacagc acagagtgag ttatggcagt
    68941 tggtcagcac atacggcagc gggaaacgtt ccataccgga cgctttagcg gtctgtacaa
    69001 taccaacaaa ggtaatatcg tgagaggcta atgaatcaaa cttaatttta agtttatcca
    69061 tatttccgga cgtattatga gaagagagaa tagaccaggc aatagtgcct ttttttgctt
    69121 cttctttttt tatttcgccg gtgaaatgtt cttcggcaat tatttcgtta ttgctggcga
    69181 gaaacacgcc tttttcagat aacttgatca tcaaatgctc cagatatctg cgcaccgtga
    69241 agtgcgcaga aagataataa atggatatac tctaaataat tcgagttgca ggaaggcgac
    69301 aagcgagtga atccccagga gcttacataa gtaagtgact ggggtgagcg aacgcagacg
    69361 cagcacatgc aacttgaagt atgacgagta tatttacagc atgttccagc cgatcagcat
    69421 attccaccac cacacgccga gggtgatatg caccaggaag gttaatatcg tcagtaccgc
    69481 accgaccaac caccaggatt taatatcgtt gtaacccacg ccaaagatga ccggacctgc
    69541 cgcgccgcca tagtgagtaa ccatgccgcc ataggagtta gagaacaaca gtgccagcgc
    69601 ggttaacatt aacggtgcgc cggagacgtt cgccagcatg gcaaatacag gtaacatagc
    69661 aacgatatag gcactaccgg aagcgaagaa ataacgcacg atgatgctga ggaaaataat
    69721 aacgaagaaa gcaacgttac cgtgaccatc aaatgccagg ttatttttaa agacttcagc
    69781 taaccattca aagaatttaa ctttcgataa taaggagctt aagccgataa taccgccgta
    69841 ccagattaag gtattccagc cgcctttatt tttaaccacg tcttcccagg taacgatacc
    69901 cagcagcagc atagtcgcca taacaacgat tgccacggtg gattcatcaa cccccagtga
    69961 cttactgaaa atccagccca gcagcgccag cacgaagaca ccgagcagca ttttttcgcg
    70021 gattttcatc ggtcccagtt cggcaaggcc cgctttggcg atggttttgt tatccacctt
    70081 cttaatttct ggcggataca tggtgtaaat caccagcggg gtgaccagca gcataatgat
    70141 acccggcaat ccagcggcca gcgcccatcc accccagcta atttgcaggt gcagaatgtc
    70201 gttgatcatt ttcagcgcca gaatgttccc cgccattgcg gtaaagaaca tatagctggt
    70261 ggttttagtg accatgtaaa tggacatcat caggtaatgt ccaacacgac gcggactttt
    70321 ttccggttct gatcccaaag ccactgccac gctgttgatg atcggtaaca cgatgccgcc
    70381 cgcacgcgcg gtgttagacg gtgttgccgg agccagtacc agatcgagaa ataccgtaac
    70441 gtaacccaga cccagggtag tgctaccaat tttaccaatc agcagatagg caatacgttt
    70501 acctaagcct gtggttacaa acgcggcgct taaagtaaac gcagaaaaca ccagccaggt
    70561 ggtaccggaa gagtaaccgc ttaatacggc ggtggtttta aattccccac ctgataagtt
    70621 acccaccact accatcgatg cggcgacggc aattaacagt acgacaggtt ccgggaaagg
    70681 cttgataacc agccccacaa tggccgccag gtaaatacca aaaagcaccc acgccagttc
    70741 gcttaatcct gccggagctg gcataaagcc aataatacat gggatcgcta atatcagaat
    70801 tagcttccat aacgattttt tattcattta atatcctgtt ctttctgtat gactcataca
    70861 gaattccttt attgattgtc attatcacga caacaaaatt tttcagggaa gataaacttc
    70921 accggaaaat atttttcttg tcgtccgaat aacagatgcg cgtaacgtcg tggcatcctg
    70981 accttcatta cttaaatgaa cgtcgagcgc accactgggg tgttcaatat tgatattgcc
    71041 gtatcctaca gaagggacga tttgtcgggt gacggtgcct tccaatgcac aactacagga
    71101 aatagcaata gcaccggtta tcgccaaagc gcgatggcaa gaatgcggca taaaataacg
    71161 cacattaatt gccccgcctt tctgcgctgg agaaataagt acaggtttag ggataaccat
    71221 attactgaca tcgcctaagc ccattgcttt acccgcttgt agacggatag attcaatgct
    71281 ggctaataat gctttgtcgg catccagctc tgccggtaat tcatagcctg tttttcccag
    71341 atattcagca ggaataatga caactggcat cgccatatcg atacaggtca ccgggacatc
    71401 gtcaaaataa tcaatctgat tatccgtcgg gaagactttt ccggttttcg ttccggcggc
    71461 attcaggaaa gtgagcgcaa ccggtgcggc agtacccggt acgccgtcaa ttctggcgct
    71521 accctcgtac tcgacaacac catttggcgt ttgcacatca gcttcgataa acgtacccgt
    71581 attgacgttg cggatacgta cgcgggtaac tggcgaagtc gctgcaatca aaccattttc
    71641 aatggcgaat gccccaacgc cagacagcat attgccgcag ttaggcgtgg tatcgacacg
    71701 ttgctcatgg acgataactt gagcaaacag ataatcgaca tctgcacgcg gatcgctgga
    71761 gcggctaata atggcgactt tactggtcag cggattaccg ccgccaatac cgtcaatttc
    71821 cagatcgtta ccggaaccca taattgccat caatatttta tcgcgctgcg tttgatcttc
    71881 gggtagatgt tccgctaaca ggaacgcgcc cctcgaggtt ccacctcgca tcatcacgca
    71941 gggtattttt ttcataataa tgccctcaat ttaaaaacat aacttattgc gatatggtta
    72001 cattaagggc aaagcatctc taatgacttt agctcaacga ttaataacat tttttcatta
    72061 atttaagttg gctaaatgca gttaaaattt ttaactgcca gtcacccaaa atcgtcgctt
    72121 ccattttccg gagtaataat gaagcatgaa ttatcaagta tgaaggcatt tgtcatactg
    72181 gcagagtcca gttcatttaa caatgctgct aaattactca atattacgca acctgcatta
    72241 acgcgaagaa taaaaaagat ggaggaagat ttacacattc aactatttga gcgtacaact
    72301 cgcaaggtta cgttaacaaa agcaggaaaa aggttgctcc cggaggcgcg ggaattaata
    72361 aaaaaattcg atgaaacgct ttttaatatt cgtgatatga atgcttatca tcgtggtatg
    72421 gtgacgttag cgtgtattcc gaccgcagta ttttattttt taccgctggc aatcggtaaa
    72481 tttaatgagc tgtatcccaa tattaaagtg cggattctgg aacaaggcac gaataattgc
    72541 atggaatcgg tgctgtgtaa cgaatcagac tttggcataa acatgaacaa cgtcacaaat
    72601 tcatccattg attttacccc actggtcaac gagccgttcg tgctggcctg tcggcgtgac
    72661 catccattgg ccaaaaagca gcttgtagaa tggcaggagt tagtgggtta caaaatgatt
    72721 ggcgtacgct cctccagcgg caaccgacta ttgatagaac aacagctagc cgacaagccc
    72781 tggaagctgg actggtttta cgaagtgcgt catctttcga cgtcattagg gctggtggag
    72841 gcgggactgg ggatttcggc gcttcctggt ctggcaatgc cccatgcgcc ctattcttcg
    72901 attattggta tcccgctggt ggaaccggtt atacgtcgga cattggggat tattcgccgc
    72961 aaagatgccg tgctttctcc ggcagctgaa cgcttttttg ccttgctgat taatttgtgg
    73021 actgacgata aagacaattt gtggaccaat atcgtcgaac gccagagaca tgcgttacag
    73081 gagataggtt aatacaccgg cgcagaagag actgcgccgg gtaaatcagc ggttagtgtg
    73141 cgttaaccac cacccacatt ggtccctgcc cgaccgcata gcggcctttt tcatgcagta
    73201 gcccctgctc gccaacaatt tcgtataccg agatgtggtg agatttttgc ccggcggcaa
    73261 tcagatactt gccgctgtga tcaacattga agccgcgcgg ctgggtttca gttggctgga
    73321 agccttcttt actcaacacg ctgccatctt ccgaaacgct gaaaacagta atcagactgg
    73381 cggtacggtc gcaggcgtat aaatgacgac catccggggt gatatgaata tcagccgccc
    73441 aacgggtgtc ggagaagttt tccggcatca tatccagcgt ctggacacat tcgatattgc
    73501 catgcggatc tttcagttcc cagacatcca ctgagctgtt taactcattg acgcaatacg
    73561 catattgttc gtttggatgg aataccatat gacgcgggcc ggccccttca acggtagtca
    73621 cttccgcagg gtcctgcgcc accagatgac catcctcgct gacagtaaac aggcaaatgc
    73681 gatcctgctt taatgccgga acccacagcg tacggttgtc cggtgagata ttggcggaat
    73741 ggcaaccgtc cagcccctcg accacatcga cgacgcccac tggcaggcca tcttccagac
    73801 gcgttacgct cacgttaccc gcattgtaag aaccgacaaa gacaaactgc ccctggtgat
    73861 cggtggaaat atgcgtcgga ctacccggca gcgcagactc tgcggcaaag gtcagcgcgc
    73921 catcgtccgg ggcgatacga tacgccagga cgcgaaactc agggcgaaca ccaacataga
    73981 gataacgttt gtccgggctg accaccatcg gctgcacctg ccccggcacg tcgacaacct
    74041 gtgtcagcgt cagtgcgcct tcatgattca ggttccagac gtgaatttgc tggctctcag
    74101 ggctggcgat ataaactgtt tgcttcatga atgctccttt gcatttagca actgactgta
    74161 acagctaaaa ttagtcgctt ttggcggtga atgctgaatt aaaacgcaga attttgtccg
    74221 atagtggtat cggtgtacca ttcgcgcaga cgaaaattct taaccttaat ctggaatacg
    74281 ccatgaccac acgcgtgatt gctctcgact tagacggcac cttattgacc ccgaaaaaga
    74341 ccttgcttcc atcatcgata gaagccctgg cccgcgctcg cgaagcaggc tatcaattaa
    74401 tcatcgtcac aggtcgccat cacgtcgcta ttcatccttt ttatcaggcg ctggcgctgg
    74461 atacacctgc tatttgctgt aatggcacct atttgtatga ttatcatgca aaaacagtgc
    74521 tggaagcgga cccaatgccc gttaataaag ccctgcaact cattgagatg ctgaatgaac
    74581 accacattca cggtctgatg tatgtcgatg atgcaatggt ctatgagcac ccgaccggac
    74641 atgtcattcg cacgtctaac tgggcgcaaa ccctgccgcc ggaacagcgt ccgactttca
    74701 cacaagtcgc ttctctggct gaaacggcgc aacaagttaa cgccgtatgg aagttcgccc
    74761 tgacgcacga tgacctgccg caattgcagc attttggtaa gcatgtcgaa catgaactgg
    74821 gactggagtg tgaatggtcc tggcacgatc aggttgatat tgcacgcggc ggcaacagca
    74881 aaggtaaacg tttgacgaaa tgggttgagg cgcaaggctg gtcgatggaa aacgtcgtgg
    74941 ccttcggcga taacttcaat gatattagta tgctggaagc cgctggtaca ggcgtggcga
    75001 tgggcaacgc cgatgacgca gtaaaagcgc gcgccaacat tgtgattggt gataacacca
    75061 ccgacagcat tgcccagttc atttatagcc acctgattta atcaggcggt tatcgacaca
    75121 cttttaattt gcgcatacag ccacaggcca ggtttgatcg ccagttcatc cctggcccac
    75181 gggctgatac gcgcccacag cgttttaccg ccgacttcca gctccacttc cacctggccg
    75241 ttgtcgtcat aactattaac cacttttgcc cgcaatacgt tacgaatgct ggtttgctgc
    75301 ggcggttgta aaaccagcga gacatcggaa gcctgaatgc ggatgcgtag cgcagcttgc
    75361 agcggttcgt ccagcttatt gacccacaaa tgctgatcgc ccagcgccag cgcggtcatc
    75421 gcgtaatgcg gatgatgctc aagcaccgtc actttcagaa tgctactttg ttgctctttc
    75481 ggcagccacg gattcatcac gctactgccc cacacttcct ccagcgcgcc aaaggctttc
    75541 acctgaccgt tttccagtac catcactctg tctgccagat ggaggatctc atccagcgaa
    75601 tggctgacat acaacatcgg aatgttgatt tcccgtgtca gccgttgcag ataaggcaac
    75661 agttcgcgtt tacgcggaat atccagtgac gccagcggtt catccagcaa cagcaattcc
    75721 ggtgctgtca gcaaagcccg accaatcgcc acgcgctgtt tttcacctcc ggacaggctg
    75781 cctggtaaac ggtcaagcaa cggttcaatg cctaaaagcg ccaccagctt atcgaactga
    75841 tcgaccatac ttttcgccat gccgtagcgc agattgccac gcactttgta atgcgggaac
    75901 agccgcgcat cctgaaaaac atagccaacg cgacgctttt ccggcgtcag gcagatacct
    75961 ttttcggcat catttagtac ccgcccattg aggacaatcc gccctttttg cgggcgcgtc
    76021 agtccactga tggcgttaat cagcgaagtt tttccggcac cggagacgcc aaagatagca
    76081 gtgatgccat tggcgggcag cgtttcatta atggtcaggc aatggttgcc caacgtctgg
    76141 gaaaaattca gttccagcat gattagcgcc ccgcccgttc acggctgatt ctggccagcc
    76201 attctgaaat caacagggag atcatcgcca gtgcaataga aataatgcac agtctcgccg
    76261 ctccactttc cccgccgggg gtctggatca gggtatacat ggcagaagga atggttcgcg
    76321 tttcaccagg aatgttcgac acaaaggtga tggttgcacc aaactcaccg agagaacgag
    76381 caaaagccag taccgtacca acaataatgc ccggtaaggt cagcggtaac gtgatagtaa
    76441 agaaaacgcg ccagcgcccg gcacccagtg ttcttgcggc ctgttccagt ttgacatcaa
    76501 ccccttccag cgccagacga attgcccgca ccatcagcgg aaacgacatg acggcggcag
    76561 cgagaaccgc gccgcgccag ctaaaggcga aggtaatgcc aaaccagtca tacagacgtt
    76621 caccgataaa tccgcgccgt cccatcgaaa ctaataataa gtaaccgacg accacgggcg
    76681 gtaacaccag cggtagatgc agtacgctgt cgagcagagc tttgcccgga aacgtgcaac
    76741 gcaccagtaa ccaggcaaaa aagatcccaa acggcaggct aaacagcaca gccagggaag
    76801 aaactttcag gcttagtaaa accgcctgcc attctggatc ggtcagtatc attacttggt
    76861 tgtaaatccg taacgtttaa agatttccgc agcctgcggt cccttcagat aatcataaaa
    76921 tgctttcacc gtcgcattgt tatgcccttc cacaacagca accggatatt ccactttttt
    76981 atgcgaatct tccgggaagg tggcaaccac ttttacccct ttgctggcaa ctgcgtcaga
    77041 accgtaaaca atacccagcg gcgcttcatt gcgttcgacc aacgccagcg cgccacgaac
    77101 atcttccgcc ggagccagtt tcggggagag cgtatcccat gcgcccagtt tttgcagtgc
    77161 ttcttttgca taaatgccag cgggaacatg ttccggatcg ccaaccgcca gacgaccgcc
    77221 attcagcagc gaagtccagt tggttttgct gtcgatggtg aaatctttct gctcgctggc
    77281 tttcggtgct acaacgacca ggctattgcc gagcagtgtc tggcgcgaag ccgtatcgat
    77341 cgctttttta tcaaccgcat aatccatcca tttctgatcg gcagaaataa acagatccgc
    77401 aggcgcaccc gcttcaatct gacgggccaa agtagatgac gaagcgaaag aagaaaccac
    77461 atccacgcct ttctcttttt tatactgcgt agcaatgtcc tgcattgcgt tagtcagtga
    77521 tgcggcggcg aacaccgtga ttttcccttc atctgccagt gcattgccag caacagcgaa
    77581 agagagtgcc gccccggcaa acaagttcaa ccatttacga gccattggta accccttaat
    77641 ctaacgttat gtaggcgaca atataacggc taactcagaa ttttcccagc agttattgct
    77701 aacctttgtg aggtagatga gaaaaaatat cggcaggaaa agcaggaggt tgagaaaaag
    77761 aaatgcccga ctaagcgggc attcagggat tcaatgattt tgtccgggct ggtctttttt
    77821 accaacacca gaaaagatgt tgaatacttc accaagaccg taaatcagac ccaggatgat
    77881 ggccatcacg acaggtacca tgattacggc gaataccaga ctttttaata actctaacat
    77941 ggtcaactcc agatatagtc atgagactat tctaaccgct aagcaaagaa aagcactccc
    78001 cttttgtgcg gtcagctttg cgtggcgttc gttttccgtc acaataagac tttttccagg
    78061 atattgttat gcaggccgaa atccttctca cccttaagct ccaacaaaaa ttattcgccg
    78121 acccgcgccg catttcgcta ctaaaacaca ttgcgctttc cggttccatt agccagggag
    78181 cgaaagatgc cggtatcagc tataaaagcg catgggatgc cattaacgag atgaatcagt
    78241 taagtgagca tattctggtt gagcgcgcaa caggcggtaa aggtggaggc ggcgcagtac
    78301 tgacccgcta tggtcagcga ctgattcagc tctatgactt actggcgcaa atccagcaaa
    78361 aagccttcga tgtgttaagt gacgatgacg ccctgccgct gaacagcctg ctggccgcga
    78421 tctcacgttt ttcactgcaa accagcgccc gtaaccaatg gtttggcacc atcaccgccc
    78481 gcaatcatga tgacgttcaa cagcatgttg atgtcttact ggctgacgga aaaacacgcc
    78541 tgaaagtcgc aattaccgca caaagcggcg cgcgtctggg gctggatgaa ggcaaagaag
    78601 tgttgatatt actaaaagcg ccgtgggtag gcattactca ggacgaagcg gtcgcacaaa
    78661 acgccgacaa ccaattaccg ggtattatta gccatattga gcgcggcgca gagcagtgcg
    78721 aagtattaat ggcgctaccc gacgggcaaa cactgtgcgc cacagtgccg gtaaatgaag
    78781 cgacttctct tcagcaagga cagaatgtca cggcctactt taatgccgac agcgtgatta
    78841 tcgccacgct gtgctaagcg tgttgacaat ttgttatgaa acacgtatcc ctgtcagtaa
    78901 tcgctgcaca aaatgggata taaaatgtca tcgttgcaaa ttttgcaagg cacgtttcgt
    78961 cttagcgaca caaaaacgct gcaattgcct cagctaacgt taaacgcggg tgatagttgg
    79021 gcgtttgtcg gttcgaatgg aagcgggaaa tcggcgctgg cccgcgcgca ggcgggggaa
    79081 cttccgcttt tgaaaggtga acggcaaagc cagttttccc acatcactcg tctctccttc
    79141 gagcaattgc aaaagctcgt cagcgacgaa tggcagcgga ataacaccga tatgctcggc
    79201 cctggcgaag atgacaccgg gcgtaccacg gctgaaatca ttcaggatga agtaaaggat
    79261 gcaccgcgtt gcatgcaact ggcgcagcag ttcggtatta ccgccctcct cgaccgacgc
    79321 tttaaatacc tttccactgg cgagacgcga aaaaccctgc tgtgtcaggc gctgatgtcg
    79381 gagcctgact tgttgattct tgatgagccg ttcgatggcc tggatgttgc ctcacgtcag
    79441 cagctggctg agctactcgc ctcgttacat cagtccggta ttactctggt actggtgctc
    79501 aatcgcttcg atgagatccc ggaatttgtc cagtttgctg gcgtgctggc ggattgtacg
    79561 ttagcggaaa cgggcgctaa agaggaactg ctccagcaag cactcgtcgc gcaactggca
    79621 catagcgaac agcttgaagg tgtgcaactg ccggagccgg atgaaccttc agcacgtcac
    79681 gccttacccg ccaacgaacc gcgcattgtg ctgaacaacg gcgtggtttc ttataacgat
    79741 cgccccattc ttaataacct tagctggcag gtgaatccag gcgagcactg gcaaattgtc
    79801 gggccaaatg gcgcgggaaa atcgacgtta ttaagcctga ttactggcga tcatccgcaa
    79861 ggttacagca acgatttgac gcttttcgga cgacgtcgcg gcagcggcga aaccatctgg
    79921 gatatcaaaa agcatatcgg ttacgtcagc agtagtttgc atctggatta ccgggtcagc
    79981 actaccgtgc gtaatgtgat cctttctggc tattttgatt cgattggcat ttatcaggcc
    80041 gtttcggatc gccagcaaaa actggtgcag cagtggctgg atattctcgg cattgataaa
    80101 cgcacggctg acgctccgtt ccatagtctt tcctggggac agcagcgtct ggcgctgatc
    80161 gtccgcgcgc tggtgaaaca tccgacgttg cttattctcg atgaaccact acaggggctt
    80221 gatccgctca atcgccagct tatccgccgg tttgttgatg tgctgattag cgaaggtgaa
    80281 acgcaattgt tgtttgtttc gcaccatgct gaagatgcgc ctgcctgtat tacccatcgt
    80341 cttgagttcg tgccggacgg tgacttttat cgctatgcgc tgacaaaaat aaactgagtc
    80401 ggtaatgctg actgtaccgg aggcggccta agcgccctct ccggcctaca gttcgacgca
    80461 tccaggcatg aaaccgcgac ttttttcaga taaaaagctc aatcattcat aaaccctctg
    80521 ttttataatc cattaatggc gcataaaaaa cggctaaatt cttgtgtaaa cgattccact
    80581 aatttattcc atgtcacact tttcgcatct ttgttatgct atggttattt cataccataa
    80641 gcctaatgga gcgaattatg agagttctgg ttaccggtgg tagcggttac attggaagtc
    80701 atacctgtgt gcaattactg caaaacggtc atgatgtcat cattcttgat aacctctgta
    80761 acagtaagcg cagcgtactg cctgttatcg agcgtttagg cggcaaacat ccaacgtttg
    80821 ttgaaggcga tatccgtaac gaagcgttga tgaccgagat cctgcacgat cacgctatcg
    80881 acactgtgat ccacttcgcc gggctgaaag ccgttggcga atcggtacaa aaaccgcttg
    80941 aatattacga caacaatgtc aacggtaccc tgcgcctgat tagcgccatg cgcgccgcga
    81001 acgtcaaaaa ctttattttt agctcctccg ccaccgtcta tggcgatcag cccaaaattc
    81061 catacgttga aagcttcccg accggcacac cgcaaagccc ttacggcaaa agcaaactga
    81121 tggtggaaca gatcctcacc gatctgcaaa aagcccagcc ggactggagc attgccctgc
    81181 tgcgctactt caacccggtt ggcgcgcatc cgtcgggcga tatgggcgaa gatccgcaag
    81241 gcattccgaa taacctgatg ccatacatcg cccaggttgc tgtaggccgt cgcgactcgc
    81301 tggcgatttt tggtaacgat tatccgaccg aagacggtac tggcgtacgc gattacatcc
    81361 acgtaatgga tctggcggac ggtcacgtcg tggcgatgga aaaactggcg aacaagccag
    81421 gcgtacacat ctataacctc ggcgctggcg taggcagcag cgtgctggac gtggttaatg
    81481 ccttcagcaa agcctgcggc aaaccggtta attatcattt tgcaccgcgt cgcgagggcg
    81541 accttccggc ctactgggcg gacgccagca aagccgaccg tgaactgaac tggcgcgtaa
    81601 cgcgcacact cgatgaaatg gcgcaggaca cctggcactg gcagtcacgc catccacagg
    81661 gatatcccga ttaaggaacg accatgacgc aatttaatcc cgttgatcat ccacatcgcc
    81721 gctacaaccc gctcaccggg caatggattc tggtttcacc gcaccgcgct aagcgcccct
    81781 ggcagggggc gcaggaaacg ccagccaaac aggtgttacc tgctcatgat ccagattgct
    81841 tcctctgcgc aggtaatgtg cgggtgacag gcgataaaaa ccccgattac accgggactt
    81901 acgttttcac taatgacttt gcggctttga tgtctgacac accagatgcg ccggaaagtc
    81961 acgatccgct gatgcgttgc cagagcgcgc gtggcaccag tcgggtgatc tgcttttcac
    82021 cggatcacag caaaacgttg ccagaactca gcgttgcggc attgacggaa atcgtcaaaa
    82081 cctggcagga gcaaaccgca gaactgggga aaacgtaccc atgggtgcag gtctttgaaa
    82141 acaaaggcgc ggcgatgggc tgctctaacc cgcatccgca cggacagatt tgggcaaata
    82201 gcttcctgcc taacgaagct gagcgcgaag accgtctgca aaaagaatat ttcgccgaac
    82261 agaaatcgcc aatgctggtg gattatgttc agcgcgagct ggcagacggt agccgtaccg
    82321 ttgtcgaaac cgaacactgg ttagccgttg taccttactg ggctgcctgg ccgttcgaaa
    82381 cgctactgct gcccaaagcc cacgttttac ggatcaccga tttgaccgac gcccagcgca
    82441 gcgatttggc actggcgttg aaaaagctga ccagtcgtta tgacaacctc ttccagtgct
    82501 ccttccccta ctctatgggc tggcacggcg cgccatttaa tggcgaagag aatcaacact
    82561 ggcagctgca cgcgcacttt tatccgcctc tgttgcgctc tgccaccgta cgtaaattta
    82621 tggttggtta tgaaatgctg gcagaaaccc agcgagacct gaccgcagaa caggccgcag
    82681 agcgtttgcg cgcagtcagc gatatccatt ttcgcgaatc cggagtgtaa gaaatgagtc
    82741 tgaaagaaaa aacacaatct ctgtttgcca acgcatttgg ctaccctgcc actcacacca
    82801 ttcaggcgcc tggccgcgtg aatttgattg gtgaacacac cgactacaac gacggtttcg
    82861 ttctgccctg cgcgattgat tatcaaaccg tgatcagttg tgcaccacgc gatgaccgta
    82921 aagttcgcgt gatggcagcc gattatgaaa atcagctcga cgagttttcc ctcgatgcga
    82981 ccattgtcgc gcatgaaaac tatcaatggg ctaactacgt tcgtggcgtg gtgaaacatc
    83041 tgcaactgcg taacaacagc ttcggcggcg tggacatggt gatcagcggc aatgtgccgc
    83101 agggtgccgg gttaagttct tccgcttcac tggaagtcgc ggtcggaacc gtattgcagc
    83161 agctttatca tttgccgctg gacggcgcac aaatcgcgct taacggtcag gaagcagaaa
    83221 accagtttgt tggctgtaac tgcgggatca tggatcagct aatttccgca ctcggcaaga
    83281 aagatcatgc cttgctgatt gactgccgct cactggggac taaagcagtt tccatgccga
    83341 aaggtgtggc tgtcgtcatc attaacagta acttcaaacg taccctggtt ggcagcgaat
    83401 acaacacccg tcgtgaacag tgcgaaactg gtgcgcgttt cttccagcag ccagcgctgc
    83461 gcgatgtcac cattgaagag ttcaacgctg ttgcgcatga actggaccca atcgtggcga
    83521 aacgcgtgcg tcatatcctg actgaaaacg cccgcaccgt tgaagctgcc agcgcactgg
    83581 agcaaggcga cctgaaacgt atgggcgagt tgatggcgga gtctcatgcc tctatgcgcg
    83641 atgatttcga aatcaccgtg ccgcaaattg acactctggt agaaatcgtc aaagctgtga
    83701 ttggcgacaa aggtggcgta cgcatgaccg gcggcggatt tggcggctgt atcgtcgcgc
    83761 tgatcccgga agagctggtg cctgccgtac agcaagctgt cgctgaacaa tatgaagcaa
    83821 aaacaggtat taaagagact ttttacgttt gtaaaccatc acaaggagca ggacagtgct
    83881 gaacgaaact cccgcactgg cacccgatgg tcagccgtac cgactgttaa ctttgcgtaa
    83941 caacgcaggg atggtagtca cgctgatgga ctggggtgcg actttacttt ccgcccgtat
    84001 tccgttttcc gatggcagcg tccgcgaggc gctgctcggc tgtgccagcc cggaatgcta
    84061 tcaggatcag gccgcatttc tgggggcctc tattggtcgt tatgccaacc gtatcgccaa
    84121 tagccgttat acctttgacg gtgaaaccgt gacgctttca ccaagtcagg gcgttaacca
    84181 gctgcacggc ggcccggaag ggttcgacaa acgtcgctgg cagattgtga accagaacga
    84241 tcgtcaggtg ctgtttgccc tgagttcaga tgatggcgat cagggcttcc cgggtaatct
    84301 cggagcgacg gtacaatatc gtctgaccga cgataaccgt atctctatta cttatcgcgc
    84361 cacagttgat aaaccttgcc cggtgaatat gactaatcac gtctatttca atcttgacgg
    84421 cgagcagtct gacgtgcgca atcacaagtt gcagattctg gcggacgaat atctgccggt
    84481 tgatgaaggc ggcattcccc acgacggcct gaaatctgtc gccggaacgt cttttgattt
    84541 ccgcagcgcc aaaatcatcg ccagtgagtt tcttgccgac gacgatcagc gcaaagtgaa
    84601 aggttacgat cacgcgttct tgttacaggc caaaggcgat ggcaagaaag tggcggcgca
    84661 tgtctggtca gcagatgaaa aattgcagct gaaggtctac accaccgctc cggctctgca
    84721 attctactcc ggcaactacc tcggcggcac accgtcgcgg ggaaccgaac cttacaccga
    84781 ctggcaaggg ctggcgttgg aaagcgagtt tctgccggac agcccgaacc accctgaatg
    84841 gccgcaaccg gactgtttcc tgcgtcctgg cgaagagtat tccagcctga cggaatatca
    84901 gtttattgct cagtaatgtt aagccctcgt catgagggct ttatcttcat attgttcaaa
    84961 tcaccagcaa acaccgacat atttgcaact caatattcac aacaacctta cactgcgaca
    85021 ctattttcgc tatggttatg cgtaagcatt gctgttgctt cgtcacggca atataatgag
    85081 aattattatc attaaaagat gatttgagga gtaagtatat ggctgtaact aagctggttc
    85141 tggttcgtca tggcgaaagt cagtggaaca aagaaaaccg tttcaccggt tggtacgacg
    85201 tggatctgtc tgagaaaggc gtaagcgaag caaaagcagc aggtaagctg ctgaaagagg
    85261 aaggttacag ctttgacttt gcttacacct ctgtgctgaa acgcgctatc cataccctgt
    85321 ggaatgtgct ggacgaactg gatcaggcat ggctgcccgt tgagaaatcc tggaaactga
    85381 acgaacgtca ctacggtgcg ttgcagggtc tgaacaaagc agaaaccgct gaaaagtatg
    85441 gcgacgagca ggtgaaacag tggcgtcgtg gttttgcagt gactccgccg gaactgacta
    85501 aagatgatga gcgttatccg ggtcacgatc cacgttacgc gaagctgagc gagaaagaac
    85561 tgccactgac ggaaagcctg gcgctgacca ttgaccgcgt gatcccttac tggaatgaaa
    85621 ccattctgcc gcgtatgaag agcggtgagc gcgtaatcat cgctgcacac ggtaactcgt
    85681 tacgtgcgct ggtgaaatat cttgataaca tgagcgaaga agagattctt gagctgaata
    85741 tcccgactgg tgtgccactg gtgtatgagt tcgacgagaa tttcaaaccg ttgaaacgct
    85801 attatctggg taatgctgac gagatcgcgg cgaaagcagc ggccgttgcg aaccagggta
    85861 aagcgaagta aacgtcattc gcttaaaaat gagaaagccg actgcaagtg agtcggcttt
    85921 tttgtttgtc gttgctaaca atggagcaca ttgcctgatg ctacgctgcg cgtcttatca
    85981 gggctgcaaa cgtacagtca tggcgattcg actataaatt acccgcgacg cgcttttact
    86041 gcattcgcca gttgacgtaa cagagcatcg gtatcatccc agccaatgca ggcatcggtg
    86101 atgctcttac cataggccag cggttcccca ctctcgaggc tctgattgcc ttccaccaga
    86161 tggctttcca ccatcacgcc aataatagcc ttttcgccac cggcaatctg ctggcaaacg
    86221 tcagcacaaa catccatctg ctttttgaat tgttttgacg agttagcatg gctgaaatcg
    86281 atcatcacct gtgctggcag gcctgctttg ttcagccctt ctttcacttc agcaacgtgc
    86341 ttcgcgctgt agttaggctc tttaccgccg cgcagaatga tatggcaatc gccgttaccg
    86401 ctggtattca caatcgccga atgcccccat ttcgttacgg acaggaagca gtgcggcgca
    86461 ccggcggcat taatggcatc gatagccact ttaatcgtac cgtcggtgcc atttttgaag
    86521 ccgaccggac aagaaagccc tgatgccagt tcgcggtgca cctgcgattc ggtggtacgt
    86581 gcgccaattg cgccccagct catcaggtca gcgagatatt gtggggtgat catatccagg
    86641 aattcacccg ccgctggcag accgctgtcg ttaatatcaa gcagcaattt acgggcaata
    86701 cgcagaccgt cgttgatctg gaagctgtta tccatatgcg gatcgttaat cagccctttc
    86761 cagcccaccg tggtacgcgg cttttcaaaa tagacgcgca tcacgatttc cagctcatct
    86821 tgcagctctt cacgcagcgt cagcaagcga gtggcatact ctttcgccgc gacaggatca
    86881 tgaattgagc acggaccaat caccaccaac aggcgatcat cattaccttt caggatctta
    86941 tggatcgctt ttcgggcatg ggcgaccgta ttcgcggcat tttcagtagc ggggaatttt
    87001 tccagcaatg cgactggagg aagtaactct ttgatttctt tgatgcgtaa atcgtcgttc
    87061 tgataattca tgtctgttcc agtgttgcca tacttatctt agtgaatgca atacttccaa
    87121 tctatatctt ccgtcagaat gtgtaaacgg ggttttacac tatgaacgga ttaatcctgg
    87181 aattagcaag aaaatcgcca gattgtcgcg aaaaacgaga tctctcctac aatttctaac
    87241 tgtaactcct ttgtttgtta attatttcaa gattctctgc tgggtatctt tgcctggctg
    87301 aaatcttaaa ccattgcctt atattcacct gcaaatgcac tgttggaaga ggttatccga
    87361 cataacgacc ataacaggag catcctatga aaatgacaaa actggccaca ctatttttga
    87421 ctgccactct aagccttgcc agcggtgccg cactggctgc cgatagcgga gcgcaaagca
    87481 ataacggcca ggcaaacgcc gcagctgatg cgggccaggt agcccctgat gcccgtgaaa
    87541 atgtcgcgcc aaataacgtc gacaataacg gggtaaatac cggttctggc ggcacaatgc
    87601 tgcatccgga tggttcttca atgaacaatg acggcatgac caaagatgaa gagcacaaga
    87661 acaccatgtg caaagatggt cgctgcccgg atatcaataa aaaagtccaa accggtgatg
    87721 gcatcaacaa tgatgtcgat accaaaaccg acggtaccac gcagtaaccc tgcgaaatag
    87781 ccaataatgg gagagcggag tctctccctt ttaatttttc ccttctaaaa gcagtatgat
    87841 gtcataaagt taagtgagaa taattaaggg atgactcgtc atggcgcact cacactcaca
    87901 cacttcctca cacctgccag aagataataa tgctcgtcgc ttgttgtatg ctttcggcgt
    87961 gactgccggg tttatgctcg tagaagtcat tggtggtttt ctttccggtt ctctggcatt
    88021 gctggccgat gcgggtcata tgttgaccga tactgccgcc ctgcttttcg ccctactcgc
    88081 agtgcaattt tcccgtcgtc ctcccaccat tcgccacact ttcggttggc taagattgac
    88141 cacgctcgcg gcgtttgtga atgccatcgc cctggtggtg attactattt tgattgtctg
    88201 ggaggcgata gaacgcttcc gcactccgcg tccggtcgag ggcggcatga tgatggcaat
    88261 tgccgtggct gggctgctgg caaatatact ttctttctgg ttacttcatc acggcagcga
    88321 agagaaaaac ctcaacgtgc gagcagcggc actgcatgtg ctcggggacc tgctgggttc
    88381 ggttggggct attattgccg cgttgattat tatctggacc ggctggacac ctgctgaccc
    88441 cattctctca atactggtgt cgcttctggt tctgcgtagc gcgtggcgac tgttgaaaga
    88501 tagcgtgaat gaattacttg aaggtgcacc ggtatcgctg gatatcgccg aactgaagcg
    88561 tcgtatgtgc cgggaaatcc cggaagtccg caatgtgcac catgtgcatg tatggatggt
    88621 aggcgagaag ccggtgatga cgctgcatgt gcaggtgatc ccgccacacg atcacgatgc
    88681 cttgttagat cagatccaac actacctgat ggatcactat cagattgagc acgccactat
    88741 tcagatggaa tatcaacctt gtcatgggcc agactgccac ctgaatgaag gggtgtctgg
    88801 ccattcacat catcaccatt aatgggacag cgcgcgtgaa cctctttcgc gtgcgctgtt
    88861 gatccacatc cggctgccgt tgagggcaat aaaggtcagg atgatgtact ccagagacat
    88921 ggcgtaaacg ccctgaagtg caaaaataac gacgctaatc acgttaataa tcacccacaa
    88981 cagccagttt tccacatact tacgcgtcat cagaatcatt gccacgatag ataacaccat
    89041 catgcatgaa tcccagaacg ggaaagcgtc cggctgtagt tcaggcatcg caacctgtaa
    89101 tcctaatgct tgcatgatca tgactgccac gcgggtcaaa aatgcaaaca ccggattgat
    89161 aaataccgtc atcagaccaa tcgaaacaac gcaaaccgcc aaccagctta gcgccttcgg
    89221 caatggcaac cagcgaattt tcaactccgc ctcgttctga ctggtttgtc gcgaccacgc
    89281 ataccagccg taaatattcg cggcaaagaa aaacatctgt aatagcaggc tggcatacag
    89341 ctgaatctga aagaaaataa tgccaaacaa ggtgacgttg atcaggccaa agaagtagtt
    89401 gctgatcttc tccagactgg caaggccaat acacagcaac ccggcgatcg tgcctaccgc
    89461 ttcgatccat gagagatcat aaccgcctgc acctattggt atatgtacca ggatattctg
    89521 cacactaaaa aaatccatct ttcccccaaa gcgtaacgtt acgccttatt attcgttatc
    89581 cacgtagtgt agccgcaaaa tccagcatac gattgagcgg caccagcgcc ctctctcgca
    89641 gcctttcatc aacatgaacc tcgtgattgc ttccttcctg ttctaatgcc tctgcgatgg
    89701 cctgaaggcc attcatggcc atccacggac aatgcgcgca gctgcggcag gttgcaccct
    89761 cacctgcggt tggtgcttcc agtaactctt taactggcac cgcctgctgc attttgtaga
    89821 aaataccccg atcggttgcc acaataagcc tctgatgtgg caatgttttc gcagcagcga
    89881 tcagttgact ggtggaaccg accgcatccg ccatctctac aatagcttgc ggtgattctg
    89941 gatgcaccag tatggcagca tccgggtatt cttcttgcaa gcgggttaac gcctgagtct
    90001 taaactcatc atgcacaatg caggcaccct gccagcataa tatgtctgcg cccgtctgtt
    90061 tttgcacgta acgccccaga tgtttgtcgg gtgcccagat gattttttca cccaaactat
    90121 ccagatgatc aataagttcg acggcaatgc ttgaagttac cacccaatct gcgcgcgctt
    90181 ttaccgcagc agaagtgttg gcgtagacga cgacagtacg atcgggatgg gcatcgcaaa
    90241 atgcgttaaa ttcttcaaca gagcagccga gatccagtga acattcagcc tgaagtgtcg
    90301 gcatcagaat tgttttttcc ggactgagaa ttttggcggt ttctcccata aatctcaccc
    90361 cagcgactaa caacgtagaa gcgggatgct ttgcaccgaa gcgcgccatt tccagagaat
    90421 cagaaataca gccaccggtt tcttctgcca gttgttgaat ttcgggatcg gtatagtagt
    90481 gggcaaccat caccgcatta cgttctttta gcagacgttt tatcttctcg cggtaatacg
    90541 ctttttcatc aatgcttaac ggcgtcggct tcgggggaaa aggataaatc gccgtgtctg
    90601 gatcaaacat cacgctcatc ttaccatctc gttttacagg cttaacgtta aaaccgacat
    90661 gagcgtaata ttcgctgtta tcggtgaaat ttgttttata tgctaaacaa gatagtcgaa
    90721 tggtggggaa aagtcactcg aattacgcct aattccaccg acgtagagtt gcttgcaaag
    90781 taagctctga agatgtcatc aggttacaaa gtgaaattga ggatgcctga atagcaaaaa
    90841 agcgccttta gggcgctttt ttacattggt gggtcgtgca ggattcgaac ctgcgaccaa
    90901 ttgattaaaa gtcaactgct ctaccaactg agctaacgac cccttgcggg attatgctgc
    90961 tttaatcatt caggatggtg ggtcgtgcag gattcgaacc tgcgaccaat tgattaaaag
    91021 tcaactgctc taccaactga gctaacgacc catacgggtg ttacctgaaa gatttctact
    91081 cggaaccaat ctttaaaatt ggtgggtcgt gcaggatgac tcgcttcgct cgccctacgg
    91141 gccgttgctg gcgcaacgtt atccttcacg cttaacatct gagtttgatg ttaaaactgg
    91201 tgggtcgtgc aggattcgaa cctgcgacca attgattaaa agtcaactgc tctaccaact
    91261 gagctaacga cccgagtggt gggtgatgac gggatcgaac cgccgacccc ctccttgtaa
    91321 gggaggtgct ctcccagctg agctaatcac ccgatactgc gctgaatact acaaactact
    91381 tccaccgaac ccaccgtaat tagtggtggg tcgtgcagga ttcgaacctg cgaccaattg
    91441 attaaaagtc aactgctcta ccaactgagc taacgacccg gtggtgggtg atgacgggat
    91501 cgaaccgccg accccctcct tgtaagggag gtgctctccc agctgagcta atcacccgat
    91561 actacgctgg atactacaac taactaggtt agtggtgggt cgtgcaggat gactcgactt
    91621 cgtctcgccc ttcgggccgt tgctgacgca acgttatcct tcacgtttta ctaccgcttc
    91681 caccttagcg attggtgggt cgtgcaggat tcgaacctgc gaccaattga ttaaaagtca
    91741 actgctctac caactgagct aacgacccac tttttcgttg ctttcggttt gtttgatatc
    91801 ccgtggcaac ggcggcatat attactgatt tcagacttga gcgcaacaaa aatttcgatg
    91861 cagatcactc aactgcttat gattcgcacg acacgaccag aaataatgcg acttctggtc
    91921 gtgtgttatg cattacatcg cgttcagacg tttttgcgcc tgtttagcgc catcagtacc
    91981 agggtattta ctgataacct gctggtatac ggctttcgct tttgcggtgt cacctttgtc
    92041 ctgcatgatg acgccgactt taaacatcgc atctgcagcc ttcggtgact tcggatagtt
    92101 tttcactacc gaagcaaaat aatacgccgc atcatctttt ttacccttgt tgtagtttaa
    92161 ctgacccagc caataattgg cgtttggcag gtaagttgaa tcagggtaat ttttgatgaa
    92221 attctgaaat gccactattg cttcatcctg gcgggattta tcctgcacca gcgcaattgc
    92281 tgcattgtaa tccgtgtttg catcgccgct tttcaccggc gcgccagcat tcgcagtacc
    92341 agcatcagct gtcggcgtcg ttgatgccgc cgcaccgctt tgatcaccgc tggttgattg
    92401 cgccgctgca ccaccgctgc tgaggctatc gatctgcaac aggatctgct tttgccgctc
    92461 cacgacctga ttcagttgat actgattttc ctgaatctga ccgcgcaggg aatcaatatc
    92521 ggattgatta tcagagagtt gttgctggag ttgggttaaa agctggctgt gagcatttga
    92581 aatacgctca agttgagtga cgcggtcttc gaccgagcct gagccgacac tactgattgg
    92641 tgcctgagca aaagcggccc agggggccgc tataccaacc agtaacgaca gactcaatag
    92701 ttgatgtctg aagttactgc tcatgcaatt ctcttagtaa accagtaccg cacgacggtt
    92761 tttggagtat gccgcttcgt catgacccag tactgcaggt ttttctttac cgtaagaaac
    92821 gatggagatc tggtctgcag aaacgccttt accctgcagg tacatcttaa cggcgttcgc
    92881 acgacgttca cccagggaga tgttgtattc cggagtacca cgttcgtccg cgtgaccttc
    92941 tacggtgact ttgtaagacg ggttgctacg caggaagttt gcatgtgcat ccagcatttg
    93001 agcgaagtca gaacggatat cgtacttgtc cagatcgaag taaacgatgt tgttctgctg
    93061 cagctgttgc atttgcagac gagcctgctc ttcggaagac atgttgccgt tgccgccgtt
    93121 cgcatccata ccagtgccgg cacccagcat gccttcgctg ccgtcattgc tggcgttctt
    93181 attggaagaa catgccgcaa ttgccataac aggcagagca atcatcagcc ctttcagcac
    93241 tttgttcagt tgcatttcaa tgattccttt actattcaat taattattat cacagatacg
    93301 gcgaccaggc agggaatttg acctgtccat cagttgccgg aagacgcgct ttgaaacgcc
    93361 catctgtaga aaccaaattc agcacggatc ccatcccctg agaagagctg tagattacca
    93421 tagtgccgtt aggtgccaga cttggcgttt catccaggaa cgtggacgac agaacttgta
    93481 cgcctcccgt tgccagatct tgtttggcaa tgtgctgctg cccaccattg gagctgacca
    93541 ttaccataaa tttaccgtcg ctgctgacat ccgcatcctg gttctgcgaa ccttcccagg
    93601 taatacgttg tggcgcaccg ccgttgatat tcactttata aacctgtgga cgaccggcct
    93661 ggtcagaagt aaatgccagg ttctggctgt ccgggaacca ggtcggttcg gtattgttac
    93721 tgcgaccatc cgtcacctgg cggatctgac cagaagccaa atccattacg tacaggttca
    93781 gactaccggt tttcgacaag gcgaatgcca gtttgctgcc gtctggcgag aatgcaggtg
    93841 caccgttgtg acgcgggaat gaagccacct gacgtacagc gccatttgcc agcgtttgga
    93901 tgaccagcgc ggaacgaccg ctttcgaagg tcacataagc cagtttagaa ccgtctggtg
    93961 accacgccgg agacatcagc ggctgcggtg aacggtgaac gacaaactgg ttgtaaccgt
    94021 catagtcaga tacgcacagt tcatacggga actgaccgcc gttggtctga acaacgtagg
    94081 caatacgggt acggaacgca cctttaatgc cggtcagttt ttcaaacact tcatcactgg
    94141 cggtatgacc agcataacgc agccactgct tgttcacttt gtacgagttc tgagcaagta
    94201 cagtacccgg tgcgccgcca gtgtcaacaa gttgataagc aacattgtaa gaaccatccg
    94261 gattcggagt gacctgaccg acaactacag cgtcaatacc cagcgcggac catgcagctg
    94321 gttgtacttc ctgcgcacta cccggctgct gtggcagacg agcgcgatct aacggattaa
    94381 atttaccgct gttacgcaag tcggcagcaa cgatgccgcc aatatcttca ggtgccgcac
    94441 cagggcccgc ccactggaaa ggaacaactc caataggacg accggaatct acaccgctgt
    94501 cgatcacaat gcggacttca gcatgcagaa ctgatgccca cagtatgaga aaaccaaatg
    94561 ctactcgtaa tgcctgcttc atcatatctc ccttatctgg accgatggcc cacgataatt
    94621 tagcagaatg ttaacaaact aaaatacaca aaactaccag aaccccgtgg caaccggtgc
    94681 ctgatgttga ccgtcggaac agtcaacatc gcgattacgg tttgaagtcc aatggcgcgt
    94741 ttttgaacac ttcatatact gcctggcttg gtggtttcgg gatcttcgca agtttagctg
    94801 ctgccaacgc agcctgacaa agtgcgggat cgccaccttc aggtttgata tccagtaaca
    94861 taccatcggg agccagtttt atgcgcagcg tacaggtttt gcctgcatag gacgatgcgt
    94921 catagaactt actttcgata gcagatttaa tctgcccggc atagttattg atatcggccc
    94981 ctgatgcgcc attgttttta gtattaccac tcccggctgg cgaagcattg ttccctttcg
    95041 cccctccccc cgttttcggt gcattcttac cagagcttag ctcaccgaaa atatcatctg
    95101 cctctgcggc agcttttgct gcagcggctt tttcagctgc tgcttttgca gctgccgctt
    95161 ttttgtctgc ggcggctttt tctgccgctg ctttcttatc agctgctgcc ttttcagcag
    95221 ccgctttttc tgctgctttt ttgtcggctg cagctttctc tgctgccgct ttcttatcag
    95281 ctgcagcctt ttcagcagcc gctttcttct cagcttctgc tttggctttt tcagcagctt
    95341 cagttgccgc tttctttctt gcttcagctg cagctgcttc tgccgcttcc gctttcttct
    95401 tcagtgccgc cgcggctgcc tcggcttttt tctgcgcttc ggctgcggct ttggcggctt
    95461 ctgcttctgc ttttttcttc gcgtctgcag ccgctttctt cgcagcttct tccgcagctt
    95521 tagcatccgc ttcggccttc gctttagcat ctgccgccgc tttcgctgcc gcttcttccg
    95581 cttgcttctg ctttaactcg gcctgttttg cggcttcttc agcctgcttt ttctgctcct
    95641 gagcagctaa ccgctctttc tcaagttgct tcaggcgttc ctgttcagcc gcttgtttct
    95701 cacgcagctc ttccgcagcc tgctgttcct tcatcttgcg ctgctcatca gaacgcttcg
    95761 cgcttgattc ctggctttgc atgcgtttgt actgctcaac taccgcacct gaatcaacca
    95821 tgacagcgtc gatggatgaa ccaccgccgc ctccggctga agcttctata ttctcatcga
    95881 acgaactcca gatcagcgcc gcaaataaga tgacatgcag cactgctgaa ataattatcg
    95941 cccgcttgag cttgtcgttt tgttcggttg cctttgacac tctcggtttc caaaaactgt
    96001 tcgcctgtta cccgctctct ttcaagcaag ggaaacgcag atgtttagat aggctgcgtc
    96061 attaaaccaa ccgatttcac acccgcacta tgtaacaagt tcagtgcttt aattatttca
    96121 tcgtaaggca catcttttgc gccaccgatc agaaagaccg ttttcgggtt ggccttgaaa
    96181 cggctggaca cttccgccac cacctgctct ggtggtaaac gctccaggcg atctttctca
    96241 accaccacgg tgtactgacc aataccagac acttcaacaa tcactggcgg attatcgtta
    96301 ctgctcaccg cctgtgattc agtagcgtct ggcagatcga cctccacgct ctgggtgatg
    96361 atgggcgctg tcgccataaa gatcagcaac agcaccagca gtacgtccag caacggtaca
    96421 atgttgattt cggacttgag atcgcgacga cctcgtccac gcgctctggc catggcttac
    96481 cccttgttgc tctcgctaac ggtaaacgcc tggcggtgca gaatcgcggt aaactcttcc
    96541 ataaagttgt cgtaattcag ttccagtttg tttacgcgct ggttgagacg gttataagcc
    96601 attaccgccg gaattgcggc gaacagacca attgccgtcg caatcaacgc ttctgcgata
    96661 ccgggcgcaa ccatttgcag tgttgcttgt tttaccgccc cgagggcgat aaaggcgtgc
    96721 atgatccccc agaccgtacc aaacagacca atatacgggc tgatggagcc aaccgtaccg
    96781 aggaacggaa tgtgcgtttc cagattttca agttcacggt tcatggagat acgcatagca
    96841 cgcgacgccc cttccactac ggcttccggc gcatggctat tggcacgatg caggcgcaca
    96901 aactctttga acccgctgta aaagatttgt tccgaaccag tcagattatc ccgtttcccc
    96961 tgactctctt gatagaggcg agagagttcg attccagacc agaatttatc ttcaaacgct
    97021 tcggcttcac gcgccgctgc gttaagaata cgggtccgct ggataataat ggcccaggat
    97081 gcgattgaaa aaccaatcaa aatcaacatg ataagtttaa ccagaaggct agccttcagg
    97141 aacaaatcaa ggatattcat gtcagtcact gcttaaactc cgcgacaata gacttgggaa
    97201 gcgcacgagg cttcattttg agtgggtcaa cgcaaacaac cagaacctct gcttcattca
    97261 gcaaagtatt ctcggcgttg acaatacgtt gcgtgaaaac caaagaggtg ccacgcattg
    97321 atgttatttc agtctgtatt tcgagcatat cgtcgagccg cgcaggtgcg taatattcca
    97381 ccgtcatttt acgtaccaca aaggcaacgc gttcagccat cagcgcctgt tgactgaagt
    97441 gatgatgacg cagcatctct gtgcgtgctc tttcataaaa agcgacgtaa ctggcgtggt
    97501 acaccacacc accggcatcg gtatcttcat agtagacgcg aaccggccat cgaaacagcg
    97561 ttgtattcac tttacatccc ggtaatgcaa caaaagttag agcttttaaa cgctgctact
    97621 atacgcgcgc gattgaggtt tgggaatgag ggcaagttaa gggagcgtaa aaatttatgg
    97681 gcctggagcg gcccataatt ttttaaagag aattctgatt taaaagaaga agaaaatcag
    97741 cccgacaatg agaacaatat cggcaagcaa tgggcaaaaa atcccttgcc aaagaacctt
    97801 ctgcggacga aagcccacgc cgtgaatcac accagcacag acggcccaca tcagcaataa
    97861 accatgccag atttccagtt cactggtctt cgcggcgaaa cgtgatgggt cccaaaacat
    97921 acatcctgct aacagaagcg ccatcacgaa ggaaagcgcc cgcagggggc gcttgtccat
    97981 taccgcatac aaggttgcga taatcttact catcagatgt cttcttgacc ggctttgcct
    98041 gattcgacgt gctcaagcgc cagcgcggtg attaccccaa acgaacaggc aagaagcgtt
    98101 cccagaatcc atgcgaaata ccacattttt agctccttac ttagtacaga gagtgggtgt
    98161 tacgttcaat atcttctttg gtgatacgac cgaacatttt ccagtaacac caggcggtgt
    98221 agagcagaat gatcggtacc agaaccaccg caacccaggt catgacgtta agcgtcagct
    98281 ggctggaagt tgcatcccac attgtcagac ttgcgttcat catggtgctg gacggcatca
    98341 caaacgggaa cattgcgata ccggctgtca ggatgatgca ggccagcgtc agggaggaga
    98401 acacaaacgc ccacgccgct ttatccatac gtgcagtcag gatggtcagc agcggcagaa
    98461 ccacacccag tgccggaata gcccacagaa ttggcgtgtt gttgaagtta accagccatg
    98521 cgccagcttc acgaaccact tctttattca gtgggttaga ggctgcgtaa tggtccattg
    98581 tcgatttcac gacataacca tcgataccgt acatcaccca tacgccagcc agtgcgaaac
    98641 agaccagtgt caccagtgcc gccacctgag ccgttgcacg ggtacgcagg tgcagttcgc
    98701 ccacggtacg catttgcaga taggttgcgc cctgagtaat gatcatccct acgctcacca
    98761 cgcctgccag caggccgaac gggttaagca ggtggaagaa gttaccggtg tagtacagac
    98821 gcagatattc atcaacgttg aacggtacgc cctgcaacag gttaccgaac gctacaccaa
    98881 tcaccaacgg cggaacgaag ctaccaatga agatgcccca gtcccacatg ttacgccagc
    98941 gggtttcttc aatcttggag cggtagtcaa aaccgaccgg acggaagaac aaagacgcca
    99001 gcacgaggat catcgccaca tagaagccgg agaacgcagc ggcatagacc atcggccagg
    99061 cagcaaagag tgcgccgccc gcggtgataa gccatacctg gttaccgtcc cagtgtggtg
    99121 caatggagtt aatcataatt cgacgctcgg tgtcgttacg accgaggaaa cgagtgagca
    99181 tgcccacccc catgtcgaaa ccgtcagtga ctgcaaaacc aatcagcaga acgccaacca
    99241 gcagccacca gataaaacgc aatacttcat aatcgatcat ttgacgactc ctgtcttagc
    99301 gtgccggctg agtagtcgtg gaagactgct caaagtgata gcgaccggtt ttcaggctgc
    99361 ttgggccgag gcgtgcaaat ttgaacatta agaacaattc tgccaccagg aacagggtat
    99421 acaggccgca aatcagcacc attgagaaga tgagatcgcc tgcggtcagt gacgagttcg
    99481 ccacagctgt cggcagcact tcaccgatag cccacggttg gcgaccatat tcagccacga
    99541 accagcccgc ttctacagca atccacggca gcggaatacc gtacagcgcg gcgcgcagca
    99601 gccatttttt ctcgccaatg cggttgcgga tgacactcca gaaagagagc gcgatgattg
    99661 ccagcagcag gaagccacac gccaccatga tacggaacgc gaagtacagc ggcgctacac
    99721 gcggaatgga gtctttggtt gcctgttgaa tctgcgcttc agtcgcatca gccacgtttg
    99781 gcgtatagcg tttcagcagc agaccgtaac cgaggtcttt cttcatgcta ttgaactggt
    99841 cacgaaccgc ctggtcggta gaaccagaac gcagttgctc gagcagagag tacgccttca
    99901 tcccgttacg aatgcgttct tcatgctgca ccatcagttc tttcaggccg ataactggag
    99961 tatccacgga acgcgttgca atgatgccca gcgcgtaagg gatctggatc gcaaatttgt
   100021 tcgtctcctc ttcctgatca ggaatgccga acagagtaaa ggcagcaggc gcaggttgcg
   100081 tttcccactc ggcttcaata gcagccagtt tggttttctg cacgtcgccc atttcgtaac
   100141 cagattcatc acccagaaca ataacagaca gaacggcagc cataccgaag ctggcagcga
   100201 tagcaaaaga gcgtttagcg aaggcgaagt cacgaccttt cagcatatac catgcgctga
   100261 taccgaggat gaacatcgcg ccagtcacat aaccagacgc tacagtgtga acgaatttca
   100321 cctgagcaac cgggttaagc accagctcgg agaagctcac catctccata cgcatagttt
   100381 caaagttgaa atcggacgcg attgggtttt gcatccagcc gttcgcaacc agaatccaca
   100441 gtgcggacag gtttgaaccg agcgccacca gccaggtgac acacatatgc tgaactttac
   100501 ccagacgatc ccaaccgaag aagaacagac ctacaaaggt ggattcgagg aagaaggcca
   100561 tcagaccttc gattgccagc ggcgcaccga agatatcccc tacatagtgg gaatagtaag
   100621 accagttagt cccgaactgg aactccatgg tcagaccggt agccacaccc agagcgaagt
   100681 tgataccaaa caacttgccc cagaacttgg tcatatcttt ataaatctgt ttgccggaaa
   100741 ggacgtagac cgtttccata atggccagca ggaacgccat accgagcgtc agtggcacaa
   100801 aaaggaagtg gtacatcgcg gtcaaggcaa actgtaagcg cgacagttcg actatatcta
   100861 acatcatgac tccttgctca tcgcatgaag actccgagag tgaaccccgt tagaaagggt
   100921 cacacgcatg ccccaataca aaattatttg ctcgcctcta tcgttgccat ttcatcaaaa
   100981 gaggaa
//
LOCUS       DAZGQL010000004        91676 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.004, whole genome shotgun sequence.
ACCESSION   DAZGQL010000004 DAZGQL010000000
VERSION     DAZGQL010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 91676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 91676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..91676
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.004"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..370)
                     /locus_tag="ACHKIR_000305"
     CDS             complement(<1..370)
                     /locus_tag="ACHKIR_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HFO9988105.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGD"
     gene            complement(440..2134)
                     /gene="oxc"
                     /locus_tag="ACHKIR_000306"
     CDS             complement(440..2134)
                     /gene="oxc"
                     /locus_tag="ACHKIR_000306"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HFO9988106.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNGIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVASAISLLAKAERPLVIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(2188..3438)
                     /gene="frc"
                     /locus_tag="ACHKIR_000307"
     CDS             complement(2188..3438)
                     /gene="frc"
                     /locus_tag="ACHKIR_000307"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HFO9988107.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(3951..4583)
                     /gene="yfdX"
                     /locus_tag="ACHKIR_000308"
     CDS             complement(3951..4583)
                     /gene="yfdX"
                     /locus_tag="ACHKIR_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HFO9988108.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQVQQTQKTAA
                     AERISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKT
                     NLNDDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVME
                     NQYLMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            4879..5154
                     /gene="ypdI"
                     /locus_tag="ACHKIR_000309"
     CDS             4879..5154
                     /gene="ypdI"
                     /locus_tag="ACHKIR_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis lipoprotein YpdI"
                     /protein_id="HFO9988109.1"
                     /translation="MKVNLMIFSLFLLVSIMACNVFAFSISGGRSETSYKETEKTSVM
                     TTTHSTKLQPSQAILFKLREDAPPLNLTEDITPPFSTKANYLIHPVR"
     gene            complement(5231..5473)
                     /gene="yfdY"
                     /locus_tag="ACHKIR_000310"
     CDS             complement(5231..5473)
                     /gene="yfdY"
                     /locus_tag="ACHKIR_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HFO9988110.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            5826..6746
                     /gene="lpxP"
                     /locus_tag="ACHKIR_000311"
     CDS             5826..6746
                     /gene="lpxP"
                     /locus_tag="ACHKIR_000311"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008951 - palmitoleoyl
                     [acyl-carrier-protein]-dependent acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009409 - response to cold [Evidence IEA];
                     GO_process: GO:0036104 - Kdo2-lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HFO9988111.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLYITPEMEGYPADENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            7102..7173
                     /gene="ypdK"
                     /locus_tag="ACHKIR_000312"
     CDS             7102..7173
                     /gene="ypdK"
                     /locus_tag="ACHKIR_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HFO9988112.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(7238..8476)
                     /gene="alaC"
                     /locus_tag="ACHKIR_000313"
     CDS             complement(7238..8476)
                     /gene="alaC"
                     /locus_tag="ACHKIR_000313"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HFO9988113.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYGIEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            8853..10550
                     /gene="ypdA"
                     /locus_tag="ACHKIR_000314"
     CDS             8853..10550
                     /gene="ypdA"
                     /locus_tag="ACHKIR_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HFO9988114.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            10565..11299
                     /gene="ypdB"
                     /locus_tag="ACHKIR_000315"
     CDS             10565..11299
                     /gene="ypdB"
                     /locus_tag="ACHKIR_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HFO9988115.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            11312..12169
                     /gene="ypdC"
                     /locus_tag="ACHKIR_000316"
     CDS             11312..12169
                     /gene="ypdC"
                     /locus_tag="ACHKIR_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO9988116.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPVRQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(12172..14667)
                     /gene="ptsP"
                     /locus_tag="ACHKIR_000317"
     CDS             complement(12172..14667)
                     /gene="ptsP"
                     /locus_tag="ACHKIR_000317"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFO9988117.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDGCSLNISGSDEEQARRVLEEYIQVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRHLMAEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLNLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQMLADKRQQQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQAFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLVKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(14692..15729)
                     /gene="ypdE"
                     /locus_tag="ACHKIR_000318"
     CDS             complement(14692..15729)
                     /gene="ypdE"
                     /locus_tag="ACHKIR_000318"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFO9988118.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQSVRITTR
                     EECKIPGLLDGDRQGNDVSTIRVDIGARSYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTVVMGPATRHGHCAASIADCRDILQMQQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(15729..16814)
                     /gene="ypdF"
                     /locus_tag="ACHKIR_000319"
     CDS             complement(15729..16814)
                     /gene="ypdF"
                     /locus_tag="ACHKIR_000319"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFO9988119.1"
                     /translation="MTLLASLRDWLKAQQLDAVLLSSRQNKQPHLGISTGSGYVVICR
                     ESAHILVDSRYYADVEARTQGYQLHLLDAMHTLATIVRQIFADEQLQTLGFEGQQVSW
                     ETAHRWQSELNAKLVSATPDVLRQLKTPEEVEKIRLACGIADRGAEHIRRFIQAGMSE
                     REIAAELEWFMRQQGAEKASFDTIVASGWRGALPHGKASDKIVAAGEFVTLDFGALYQ
                     GYCSDMTRTLLVNGEGVSAESHPLFNVYQIVLQAQLAAISAIRPGVRCQQVDDAARRV
                     ITEAGYGDYFGHNTGHAIGIEVHEDPRFSPRDTTTLQPGMLLTVEPGIYLPGQGGVRI
                     EDVVLVTPQGVEVLYAMPKTVLLTGEA"
     gene            complement(16829..18076)
                     /gene="fryC"
                     /locus_tag="ACHKIR_000320"
     CDS             complement(16829..18076)
                     /gene="fryC"
                     /locus_tag="ACHKIR_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFO9988120.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKTSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPADIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFSAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(18098..18424)
                     /gene="fryB"
                     /locus_tag="ACHKIR_000321"
     CDS             complement(18098..18424)
                     /gene="fryB"
                     /locus_tag="ACHKIR_000321"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFO9988121.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(18643..19608)
                     /gene="glk"
                     /locus_tag="ACHKIR_000322"
     CDS             complement(18643..19608)
                     /gene="glk"
                     /locus_tag="ACHKIR_000322"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HFO9988122.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKIEVQDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEGIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            19812..21068
                     /gene="yfeO"
                     /locus_tag="ACHKIR_000323"
     CDS             19812..21068
                     /gene="yfeO"
                     /locus_tag="ACHKIR_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HFO9988123.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     QMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMHQMKNPVLVLGIGGFILGILGVMGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            21183..21509
                     /gene="ypeC"
                     /locus_tag="ACHKIR_000324"
     CDS             21183..21509
                     /gene="ypeC"
                     /locus_tag="ACHKIR_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein YpeC"
                     /protein_id="HFO9988124.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(21650..22888)
                     /gene="mntH"
                     /locus_tag="ACHKIR_000325"
     CDS             complement(21650..22888)
                     /gene="mntH"
                     /locus_tag="ACHKIR_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFO9988125.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            23224..24426
                     /gene="nupC"
                     /locus_tag="ACHKIR_000326"
     CDS             23224..24426
                     /gene="nupC"
                     /locus_tag="ACHKIR_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HFO9988126.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(24475..26664)
                     /gene="pdeA"
                     /locus_tag="ACHKIR_000327"
     CDS             complement(24475..26664)
                     /gene="pdeA"
                     /locus_tag="ACHKIR_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HFO9988127.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRFISPLAIIHSSYIFL
                     AWMPLCVMLSILFIFGWRGVVPVLCGMFCTNLWNFHLSFLQTAVMLGSQTFVVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVAPIGIKCSMYLVGNFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLARMIVSPHFAQILWRRDIAPSLSKEKRAFTLS
                     WLAALSVLLLLMCTPYENDFIAGYLVPVFFIIFTLGVGKLRYPFLNLTWAVSTLCLLN
                     YNQNFLQGVLTEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFRALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLMMRVHCIRSIYRTLLPLMQENEK
                     LYQLPGSELLLVLSGPETEGRLQHMVNILNSRQIHWNNTGLDMGYGAAWGRFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALDQGDLLLYA
                     QPIRNKEGEGYDEILARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLLKWLATH
                     PCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYHISPQAVILEITEEQAFSNAESSM
                     YNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVRS
                     ITDLAKAKSLSVVAEFVETQQQQALLHKLGVQYLQGYLIGRPQPLAD"
     gene            complement(26872..26947)
                     /locus_tag="ACHKIR_000328"
     tRNA            complement(26872..26947)
                     /locus_tag="ACHKIR_000328"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(26912..26914),aa:Ala,seq:ggc)
     gene            complement(26987..27062)
                     /locus_tag="ACHKIR_000329"
     tRNA            complement(26987..27062)
                     /locus_tag="ACHKIR_000329"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27027..27029),aa:Ala,seq:ggc)
     gene            27283..27642
                     /gene="yfeC"
                     /locus_tag="ACHKIR_000330"
     CDS             27283..27642
                     /gene="yfeC"
                     /locus_tag="ACHKIR_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFO9988128.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            27644..28036
                     /gene="yfeD"
                     /locus_tag="ACHKIR_000331"
     CDS             27644..28036
                     /gene="yfeD"
                     /locus_tag="ACHKIR_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003834896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFO9988129.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLLEAEEPLAEYAPGIRTPAYRQIISAIDNMTH
                     SEQEKVAQFLSREGIRNFLARLDIDESE"
     gene            complement(28087..29502)
                     /gene="gltX"
                     /locus_tag="ACHKIR_000332"
     CDS             complement(28087..29502)
                     /gene="gltX"
                     /locus_tag="ACHKIR_000332"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFO9988130.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLTAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALAFIAERENQQ"
     gene            29761..29836
                     /locus_tag="ACHKIR_000333"
     tRNA            29761..29836
                     /locus_tag="ACHKIR_000333"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29794..29796,aa:Val,seq:tac)
     gene            29881..29956
                     /locus_tag="ACHKIR_000334"
     tRNA            29881..29956
                     /locus_tag="ACHKIR_000334"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29914..29916,aa:Val,seq:tac)
     gene            30003..30078
                     /locus_tag="ACHKIR_000335"
     tRNA            30003..30078
                     /locus_tag="ACHKIR_000335"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30036..30038,aa:Val,seq:tac)
     gene            30083..30158
                     /locus_tag="ACHKIR_000336"
     tRNA            30083..30158
                     /locus_tag="ACHKIR_000336"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30116..30118,aa:Lys,seq:ttt)
     gene            complement(30269..31153)
                     /gene="xapR"
                     /locus_tag="ACHKIR_000337"
     CDS             complement(30269..31153)
                     /gene="xapR"
                     /locus_tag="ACHKIR_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XapR"
                     /protein_id="HFO9988131.1"
                     /translation="MERVYRTDLKLLRYFLAVAEELHFGRAAARLNMSQPPLSIHIKE
                     LENQLGTQLFIRHSRSVVLTHAGKILMEESRRLLVNANNVLARVEQIGRGEAGRIELG
                     VVGTAMWGRMRPVMRRFLRENPNVEVLFREKMPAMQMALLERRELDAGIWRMATEPPT
                     GFTSLRLHESAFLVAMPEEHHLSSFSTVPLEALRDEYFVTMPPVYTDWDFLQRVCQQV
                     GFSPVVIREVNEPQTVLAMVSMGIGITLIADSYAQMNWPGVIFRPLKERIPADLYIVY
                     ETQQVTPALVKLLAALAQ"
     gene            complement(31194..31352)
                     /locus_tag="ACHKIR_000338"
     CDS             complement(31194..31352)
                     /locus_tag="ACHKIR_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1427 family protein"
                     /protein_id="HFO9988132.1"
                     /translation="MKDILFSSGVGFGIGALFTIVRLPIPVPNVLPGILSIVFMYVGY
                     LVVKYFMP"
     gene            complement(31405..32661)
                     /gene="xapB"
                     /locus_tag="ACHKIR_000339"
     CDS             complement(31405..32661)
                     /gene="xapB"
                     /locus_tag="ACHKIR_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine/proton symporter XapB"
                     /protein_id="HFO9988133.1"
                     /translation="MSIALRLKVMSFLQYFIWGSWLVTLGSYMINTLHFTGANVGMVY
                     SSKGIAAIIMPGIMGIIADKWLRAERAYMLCHLVCAGVLFYAASVTDPDMMFWVMLVN
                     AMAFMPTIALSNSVSYSCLAQAGLDPVTAFPPIRVFGTVGFIVAMWAVSLLHLELSSL
                     QLYIASGASLLLSAYALTLPKIPVAEKKATTSLASKLGLDAFVLFKNPRMAIFFLFAM
                     MLGAVLQITNVFGNPFLHDFARNPEFADSFVVKYPSILLSVSQMAEVGFILTIPFFLK
                     RFGIKTVMLMSMVAWTLRFGFFAYGDPSPTGFILLLLSMIVYGCAFDFFNISGSVFVE
                     QEVDSSIRASAQGLFMTMVNGVGAWVGSILSGMAVDYFSVDGVKDWHTIWLMFAGYAL
                     FLAVIFFFGFKYNHDPEKITHRSVTH"
     gene            complement(32722..33555)
                     /gene="xapA"
                     /locus_tag="ACHKIR_000340"
     CDS             complement(32722..33555)
                     /gene="xapA"
                     /locus_tag="ACHKIR_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0055086 - nucleobase-containing small
                     molecule metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine phosphorylase"
                     /protein_id="HFO9988134.1"
                     /translation="MSQAQFSHNPLYCVDIIKTYKPDFTPRVAFILGSGLGALADQIE
                     NAVAISYEKLPGFPVSTVHGHAGELVLGYLQGVPVACMKGRGHFYEGRGMTIMTDAIR
                     TFKLLGCELLFCTNAAGSLRPEVGAGSLVALKDHINTMPGTPMVGLNDERFGERFFSL
                     ANAYDAEYRALLQKVAKEEGFPLTEGVFVSYPGPNFETAAEIRMMQIIGGDVVGMSVV
                     PEVISARHCELKVVAVSAITNMAEGLSDVKLSHAQTLAAAELSKQNFINLICGFLRKI
                     A"
     gene            33803..34567
                     /gene="yfeN"
                     /locus_tag="ACHKIR_000341"
     CDS             33803..34567
                     /gene="yfeN"
                     /locus_tag="ACHKIR_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpK"
                     /protein_id="HFO9988135.1"
                     /translation="MKKHLFTLTLSSVLAIPAVSHAEFKGGFADIGIHYLDWTSRTTE
                     KSSTKSHKDDFGYLELEGGANFSWGEMYGFFDWENFYNDRHDKPGSEQRYTFKNTNRI
                     YLGDTGFNLYLHAYGTYGSANRVNFHDDMFLYGIGYNFTGSGWWFKPFFAKRYTDQTY
                     YTGDNGYVAGWVAGYSFMLGSEKFTLTNWNEYEFDRDATYAAGNGGKEGLNGAVALWW
                     NATSHITTGIQYRYADDKLGEDFYQDAIIYSIKFNF"
     gene            complement(34605..35531)
                     /gene="yfeR"
                     /locus_tag="ACHKIR_000342"
     CDS             complement(34605..35531)
                     /gene="yfeR"
                     /locus_tag="ACHKIR_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO9988136.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFILHDRPQQWVMESIRQGDVDFGIVIDPGPVSDLQCE
                     AILSEPFFLLCHRDSALAAEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAQALWEVVRGQGRALMAGREGDPLYQI"
     gene            35621..36619
                     /gene="yfeH"
                     /locus_tag="ACHKIR_000343"
     CDS             35621..36619
                     /gene="yfeH"
                     /locus_tag="ACHKIR_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HFO9988137.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(36616..36834)
                     /gene="ypeB"
                     /locus_tag="ACHKIR_000344"
     CDS             complement(36616..36834)
                     /gene="ypeB"
                     /locus_tag="ACHKIR_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HFO9988138.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(36836..38851)
                     /gene="ligA"
                     /locus_tag="ACHKIR_000345"
     CDS             complement(36836..38851)
                     /gene="ligA"
                     /locus_tag="ACHKIR_000345"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFO9988139.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KSNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLMQFKAWGLPVSDRVTLCESAEEVLAFYHEVEKD
                     RPTLGFDIDGVVIKVNSLAQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAVKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPVVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDETEMLRLLGS"
     gene            complement(38922..39920)
                     /gene="zipA"
                     /locus_tag="ACHKIR_000346"
     CDS             complement(38922..39920)
                     /gene="zipA"
                     /locus_tag="ACHKIR_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HFO9988140.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQLVQQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSA
                     PQPAQQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLN
                     SIQQAGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQV
                     PSYGDELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            40150..40911
                     /gene="cysZ"
                     /locus_tag="ACHKIR_000347"
     CDS             40150..40911
                     /gene="cysZ"
                     /locus_tag="ACHKIR_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HFO9988141.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            41096..42067
                     /gene="cysK"
                     /locus_tag="ACHKIR_000348"
     CDS             41096..42067
                     /gene="cysK"
                     /locus_tag="ACHKIR_000348"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFO9988142.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            42451..42708
                     /gene="ptsH"
                     /locus_tag="ACHKIR_000349"
     CDS             42451..42708
                     /gene="ptsH"
                     /locus_tag="ACHKIR_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HFO9988143.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            42753..44480
                     /gene="ptsI"
                     /locus_tag="ACHKIR_000350"
     CDS             42753..44480
                     /gene="ptsI"
                     /locus_tag="ACHKIR_000350"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HFO9988144.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     TDLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            44521..45030
                     /gene="crr"
                     /locus_tag="ACHKIR_000351"
     CDS             44521..45030
                     /gene="crr"
                     /locus_tag="ACHKIR_000351"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFO9988145.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(45072..45923)
                     /gene="pdxK"
                     /locus_tag="ACHKIR_000352"
                     /gene_synonym="thiJ"
     CDS             complement(45072..45923)
                     /gene="pdxK"
                     /locus_tag="ACHKIR_000352"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HFO9988146.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            46028..46396
                     /gene="yfeK"
                     /locus_tag="ACHKIR_000353"
     CDS             46028..46396
                     /gene="yfeK"
                     /locus_tag="ACHKIR_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001592994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HFO9988147.1"
                     /translation="MKKIICLVITLLMTLPVYAKLTAHEEARINAMLEGLAQKKDLIF
                     VRNGDDHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKMPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            complement(46399..47310)
                     /gene="cysM"
                     /locus_tag="ACHKIR_000354"
     CDS             complement(46399..47310)
                     /gene="cysM"
                     /locus_tag="ACHKIR_000354"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HFO9988148.1"
                     /translation="MSTLEQTIGNTPLVKLQRMAPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MLAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPFAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(47444..48541)
                     /gene="cysA"
                     /locus_tag="ACHKIR_000355"
     CDS             complement(47444..48541)
                     /gene="cysA"
                     /locus_tag="ACHKIR_000355"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HFO9988149.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPDQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(48531..49406)
                     /gene="cysW"
                     /locus_tag="ACHKIR_000356"
     CDS             complement(48531..49406)
                     /gene="cysW"
                     /locus_tag="ACHKIR_000356"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HFO9988150.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(49406..50239)
                     /gene="cysT"
                     /locus_tag="ACHKIR_000357"
     CDS             complement(49406..50239)
                     /gene="cysT"
                     /locus_tag="ACHKIR_000357"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HFO9988151.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFRKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(50239..51255)
                     /gene="cysP"
                     /locus_tag="ACHKIR_000358"
     CDS             complement(50239..51255)
                     /gene="cysP"
                     /locus_tag="ACHKIR_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HFO9988152.1"
                     /translation="MAVNLLKKNSLVLVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMGKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRK"
     gene            complement(51413..52204)
                     /gene="ucpA"
                     /locus_tag="ACHKIR_000359"
     CDS             complement(51413..52204)
                     /gene="ucpA"
                     /locus_tag="ACHKIR_000359"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HFO9988153.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPM
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(52333..53190)
                     /gene="murR"
                     /locus_tag="ACHKIR_000360"
     CDS             complement(52333..53190)
                     /gene="murR"
                     /locus_tag="ACHKIR_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HFO9988154.1"
                     /translation="MLYLTKIRNAESEFTGNEQKIADFLRANVSELKSVSSRKMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKEQALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSELVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     TDSPLRRLAHYTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIER
                     SSELTQRLK"
     gene            53354..54250
                     /gene="murQ"
                     /locus_tag="ACHKIR_000361"
     CDS             53354..54250
                     /gene="murQ"
                     /locus_tag="ACHKIR_000361"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFO9988155.1"
                     /translation="MQLEKMITEGSNAASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSQEGGVNDLKNIGLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGPEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCSAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            54254..55678
                     /gene="murP"
                     /locus_tag="ACHKIR_000362"
     CDS             54254..55678
                     /gene="murP"
                     /locus_tag="ACHKIR_000362"
                     /EC_number="2.7.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HFO9988156.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPTATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPLGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            55683..56984
                     /gene="pbp4b"
                     /locus_tag="ACHKIR_000363"
     CDS             55683..56984
                     /gene="pbp4b"
                     /locus_tag="ACHKIR_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HFO9988157.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMD
                     RWISQQVDAGYPGVNLLIIKDNQIVYRKAWGAAKKYDGSALMAQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDRIAKYIPGFADSPNDAIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVAGALYSQDKGQTLEMIKRTPLEYQPCSKHIYSDVDYMLLGFIVESV
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     TLWGQVHDEKAFYSMGGVSGHAGLFSDTGDIAVLMQTMLNGGGYGDVQLFNAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMAIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQ"
     gene            complement(57042..57941)
                     /gene="yfeX"
                     /locus_tag="ACHKIR_000364"
     CDS             complement(57042..57941)
                     /gene="yfeX"
                     /locus_tag="ACHKIR_000364"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HFO9988158.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSIHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(58037..58612)
                     /gene="yfeY"
                     /locus_tag="ACHKIR_000365"
     CDS             complement(58037..58612)
                     /gene="yfeY"
                     /locus_tag="ACHKIR_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HFO9988159.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWSGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(58673..59122)
                     /gene="yfeZ"
                     /locus_tag="ACHKIR_000366"
     CDS             complement(58673..59122)
                     /gene="yfeZ"
                     /locus_tag="ACHKIR_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 family protein"
                     /protein_id="HFO9988160.1"
                     /translation="MKSTEFHPVHYDAYGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRIWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(59109..59534)
                     /gene="ypeA"
                     /locus_tag="ACHKIR_000367"
     CDS             complement(59109..59534)
                     /gene="ypeA"
                     /locus_tag="ACHKIR_000367"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HFO9988161.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            59748..60617
                     /gene="amiA"
                     /locus_tag="ACHKIR_000368"
     CDS             59748..60617
                     /gene="amiA"
                     /locus_tag="ACHKIR_000368"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HFO9988162.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKEEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSRKR"
     gene            60621..61520
                     /gene="hemF"
                     /locus_tag="ACHKIR_000369"
     CDS             60621..61520
                     /gene="hemF"
                     /locus_tag="ACHKIR_000369"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HFO9988163.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLSAVDGTEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEDDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKTMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPEDGSPEAALSEFIKVRDWV"
     gene            complement(61454..62578)
                     /gene="eutR"
                     /locus_tag="ACHKIR_000370"
     CDS             complement(61454..62578)
                     /gene="eutR"
                     /locus_tag="ACHKIR_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001735543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HFO9988164.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEEAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWGGGSYTQSLTLINSLNAASGLPSSG"
     gene            complement(62624..63124)
                     /gene="eutK"
                     /locus_tag="ACHKIR_000371"
     CDS             complement(62624..63124)
                     /gene="eutK"
                     /locus_tag="ACHKIR_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HFO9988165.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(63137..63796)
                     /gene="eutL"
                     /locus_tag="ACHKIR_000372"
     CDS             complement(63137..63796)
                     /gene="eutL"
                     /locus_tag="ACHKIR_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HFO9988166.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            complement(63806..64693)
                     /gene="eutC"
                     /locus_tag="ACHKIR_000373"
     CDS             complement(63806..64693)
                     /gene="eutC"
                     /locus_tag="ACHKIR_000373"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HFO9988167.1"
                     /translation="MDQKQIEEIVRSVMASMGQAAPAPSEAKCATTTCTTPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRAGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            complement(64714..66075)
                     /gene="eutB"
                     /locus_tag="ACHKIR_000374"
     CDS             complement(64714..66075)
                     /gene="eutB"
                     /locus_tag="ACHKIR_000374"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
                     /protein_id="HFO9988168.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            complement(66087..67490)
                     /gene="eutA"
                     /locus_tag="ACHKIR_000375"
     CDS             complement(66087..67490)
                     /gene="eutA"
                     /locus_tag="ACHKIR_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HFO9988169.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKTLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMTLSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTEARSLTGAQL
                     VQVTRRMAELIVEVIDGTLSPLAQALMQTGLLPVGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDEKDLVSAWQQALIQLDLDPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            complement(67487..68713)
                     /gene="eutH"
                     /locus_tag="ACHKIR_000376"
     CDS             complement(67487..68713)
                     /gene="eutH"
                     /locus_tag="ACHKIR_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0034228 - ethanolamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034229 - ethanolamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HFO9988170.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVLIVAVLVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKEDASAAKTEAEAQS"
     gene            complement(68930..70117)
                     /gene="eutG"
                     /locus_tag="ACHKIR_000377"
     CDS             complement(68930..70117)
                     /gene="eutG"
                     /locus_tag="ACHKIR_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HFO9988171.1"
                     /translation="MQSELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGAVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRDRFSLVGRALRTKKSDDRD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAALEDICLRSNPRTASLEQIVGLYA
                     AAQ"
     gene            complement(70107..70943)
                     /gene="eutJ"
                     /locus_tag="ACHKIR_000378"
     CDS             complement(70107..70943)
                     /gene="eutJ"
                     /locus_tag="ACHKIR_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HFO9988172.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQPVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGDEIWPAVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKTEGIY
                     AK"
     gene            complement(70954..72357)
                     /gene="eutE"
                     /locus_tag="ACHKIR_000379"
     CDS             complement(70954..72357)
                     /gene="eutE"
                     /locus_tag="ACHKIR_000379"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004029 - aldehyde dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0008774 - acetaldehyde dehydrogenase
                     (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HFO9988173.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPPAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQSVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLKVPEQTRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPCIAGLGLGGEGWTTMTITTPTGEGVTSAR
                     TFVRLRRCVLVDAFRIV"
     gene            complement(72369..72656)
                     /gene="eutN"
                     /locus_tag="ACHKIR_000380"
     CDS             complement(72369..72656)
                     /gene="eutN"
                     /locus_tag="ACHKIR_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HFO9988174.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            complement(72763..73056)
                     /gene="eutM"
                     /locus_tag="ACHKIR_000381"
     CDS             complement(72763..73056)
                     /gene="eutM"
                     /locus_tag="ACHKIR_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HFO9988175.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            complement(73095..74111)
                     /gene="pta"
                     /locus_tag="ACHKIR_000382"
     CDS             complement(73095..74111)
                     /gene="pta"
                     /locus_tag="ACHKIR_000382"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFO9988176.1"
                     /translation="MIIERCRKLALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFALRWLARAGEKTPPDALDKLTD
                     PLMFAAAMVSAGTADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRAITGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVQRQTEVNRESSLQ
                     TLVE"
     gene            complement(74108..74911)
                     /gene="eutT"
                     /locus_tag="ACHKIR_000383"
     CDS             complement(74108..74911)
                     /gene="eutT"
                     /locus_tag="ACHKIR_000383"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006580 - ethanolamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HFO9988177.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLAFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGE
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKQSLTVKQIQQRLGETQ"
     gene            complement(74908..75609)
                     /gene="eutQ"
                     /locus_tag="ACHKIR_000384"
     CDS             complement(74908..75609)
                     /gene="eutQ"
                     /locus_tag="ACHKIR_000384"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HFO9988178.1"
                     /translation="MKKLITANDIREAHARGELAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASASADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGALQPSFKSVTGKGGIKVIDGSSVKFGRFDGAEPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            complement(75584..76063)
                     /gene="eutP"
                     /locus_tag="ACHKIR_000385"
     CDS             complement(75584..76063)
                     /gene="eutP"
                     /locus_tag="ACHKIR_000385"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HFO9988179.1"
                     /translation="MKRIAFVGTVGAGKTTLFNALQGNYTLARKTQAVEFNDNGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(76076..76411)
                     /gene="eutS"
                     /locus_tag="ACHKIR_000386"
     CDS             complement(76076..76411)
                     /gene="eutS"
                     /locus_tag="ACHKIR_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HFO9988180.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            complement(76704..78983)
                     /gene="maeB"
                     /locus_tag="ACHKIR_000387"
     CDS             complement(76704..78983)
                     /gene="maeB"
                     /locus_tag="ACHKIR_000387"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HFO9988181.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYRGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRERAPELMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            79272..80222
                     /gene="tal"
                     /locus_tag="ACHKIR_000388"
     CDS             79272..80222
                     /gene="tal"
                     /locus_tag="ACHKIR_000388"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFO9988182.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            80242..82245
                     /gene="tkt"
                     /locus_tag="ACHKIR_000389"
     CDS             80242..82245
                     /gene="tkt"
                     /locus_tag="ACHKIR_000389"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFO9988183.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKYAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(82348..83391)
                     /gene="ypfG"
                     /locus_tag="ACHKIR_000390"
     CDS             complement(82348..83391)
                     /gene="ypfG"
                     /locus_tag="ACHKIR_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HFO9988184.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGRAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNSGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(83517..84092)
                     /gene="nudK"
                     /locus_tag="ACHKIR_000391"
     CDS             complement(83517..84092)
                     /gene="nudK"
                     /locus_tag="ACHKIR_000391"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HFO9988185.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKTVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDSQRANAGGGVEDEDIEVLELPF
                     NKALEMIKTGEIRDGKTVLLLNYLQMSHLMD"
     gene            complement(84160..86139)
                     /gene="aegA"
                     /locus_tag="ACHKIR_000392"
     CDS             complement(84160..86139)
                     /gene="aegA"
                     /locus_tag="ACHKIR_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HFO9988186.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDAALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QVQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVEVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLNSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPVEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            86345..88045
                     /gene="narQ"
                     /locus_tag="ACHKIR_000393"
     CDS             86345..88045
                     /gene="narQ"
                     /locus_tag="ACHKIR_000393"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HFO9988187.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYRSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGDNWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            88209..91322
                     /gene="acrD"
                     /locus_tag="ACHKIR_000394"
     CDS             88209..91322
                     /gene="acrD"
                     /locus_tag="ACHKIR_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HFO9988188.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAQLVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNKIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVA
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(91421..91480)
                     /gene="ypfM"
                     /locus_tag="ACHKIR_000395"
     CDS             complement(91421..91480)
                     /gene="ypfM"
                     /locus_tag="ACHKIR_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HFO9988189.1"
                     /translation="MIERELGNWKDFIEVMLRK"
BASE COUNT        22094 a        24809 c        22929 g        21844 t
ORIGIN      
        1 aatcaccata aataggatcg agaactgcaa atcccaggaa tccaatggta ggtgaacctg
       61 caattaatgc acatactgct gcttctgcat gagtacgttt aaaaaatttg tagcaaccga
      121 accaggagaa aaagaaacat ccgacaataa ccacaagtga tacaagggtc agacgagtgt
      181 ccgcaaaaat catttccctg tttgcccggg taatagatac aaatagagct gcaggaagag
      241 cgtagtttaa taccagttta ttaaatgccc gggcttgatc ttctgaaaaa gtttctcgtc
      301 tgccgctaaa ataacccaac agcatgatta cgataatagg caataaatcg ccaataaaaa
      361 atgttagcat aatagtattt ccgataaggg tcccctcacc atcgcataat gagttggcaa
      421 gaggagacga tgtcagtatt taattaccag cgacttgttt tgggttaagt ttggtaatat
      481 ggccactttc agttcctgct gcagggtcga tgaccacatt aataatggtc ggtttgcgcg
      541 actgaatacc ggtggttaaa gcatgacgaa gttcatctgt cgtagtgacg ttatagccaa
      601 cgccacgaaa cgcatccatt aatttgtcat accttgcatg gtgcagcaga tccgttggtg
      661 atggtgcacc agcgccgctg agatcaacac cgtctcctct gtagatgccg ccattattaa
      721 aaataacgat cgtcaccggc aggttatatc gacaaatcgt ttcaatttcc atcccactga
      781 aaccaaaagc actatcacct tcaatggcaa caaccggaga accagaggtc acgctagcac
      841 cgatggcata gcccataccg atgcccatga caccccaggt gccacaatcc agacgacgac
      901 gtggtttata catatcaata atatttcgtg cattatccag ggtatttgcc ccttcattaa
      961 ctaaataaat atcctggttc tcgcgcaata cgtcgcgcac agcacttaat gcattaaagt
     1021 aatttaatgg ttgagtatct gtgcttaact tttcatgcat tttttgtgca ttttgctgct
     1081 tgtggatatt taaaatatcg cgccatacca gtggagtcgt aaatgtgttt tgtttcagtt
     1141 ctgccagcat accttgcata ctggatgcaa tatcgccaac gactggcaca gcaatggggc
     1201 ggttgctgtc aatttcctgc ggttcaatat ccagttgaat aaactgtgta tctgccgccc
     1261 atcctttttt accgtgtgcc agtaaccaat tcagtcgtgc accaacaagc atgacaacgt
     1321 cagcatttgc cagggcaaac gaacgcgcag ccgccgcaga aagtggatgc gtatcttcga
     1381 ggatcccttt cgccatagac attggcagga atggaatctg ggcactttca ataaattcac
     1441 gaagctgttc atccgcttgt gaatacgccg cgcctttgcc aaggataact aatggtcgtt
     1501 cagcttttgc taatagcgaa attgcgctag cgactgactt cgggcatggc aataatgctg
     1561 gcgacggatt ttcaactttt acaatcgtgg ttaacgcttc gtctttttcc atcgtcgcgg
     1621 ccaggacatt tgctggcaaa tcaagataaa ctccgccagg gcgacccgat actgagaccc
     1681 ggatagcgcg tgccaatgca atgccaagat cctgcggctg attaacgcgg aatgctgctt
     1741 tggcatacgg ttttgccgca ttcatttggt ccagctcttc ataatcacct tgctgtaggt
     1801 cgacgatcgc gcggtcgctg gagccgctaa tcatgatcat cggaaaacca tttaccgttg
     1861 cgttggccaa tgcggtcaaa ccattgagga atcctggcgc agaaactgtc aggcagatcc
     1921 ccggtttttg ggtaagaaaa ccgcttgccg cagcggcata gcctgccgac tgctcatgac
     1981 gaaaaccaat ataacgaatg ccttccgcct gggcatggcg cgccatatcc gtcacaggaa
     2041 tacctacaac accataaata gtgtcaatac cattctgttt taatgcttca acgatgatat
     2101 gcataccatc tgtcatttga agttgatctg acatgattaa taacctcgaa atttttataa
     2161 attgggagcc agaagccccc gttaatatca gatggcgtgg ttttgcttca ttgcagcaat
     2221 ttcatcatcg ctataaccca gctcctgcaa tacagcagcg gtatgctcac ctaatagcgg
     2281 cgcagcttta atatccggcg taaaggcaga gaatttcatt ggacaaccaa cggtcagata
     2341 ttttccacgc aacggttgtt ccacttcgac aacactacca ctttggcgca aagagggatc
     2401 aagtgaaatt tctttcatac ttaaaaccgg tgcacaagga atatcaaact gagtcaaata
     2461 ggccaccgct tcatgtttat caatagtgac agtgtatttt tcgatttcag caaaaatatc
     2521 gaaaatatgt ggctgtcggg catgggctgt actgtatgcc ggatcggtaa tccattctgg
     2581 tttgccgatg gctttacagg tgttttccca gttttgctcc tgaatagtga aataaatata
     2641 ggcgttagga tcggtttccc agcctttaca tttcaggatc cagccaggct gaccgccacc
     2701 acccgcatta ccaccgcggg gaactgcatc accaaatgtg ccattcggat actgcgggta
     2761 ttcttccaga taccccaact tatcgagacg ctgctggtcg cgtaatttca cgcggcaaag
     2821 gttcaacacg gcatcctgca ttgacatggt gactcgttgc ccacgccccg ttttttcgcg
     2881 atgcagcaaa gcagcaagta aaccgatcag caaatgcatt ccggtgttgc tgtcacccaa
     2941 cgctgcagcg cttaccagcg gcggaccgtc ccaaaaacct gtagtagatg ccgcgccacc
     3001 cgctgcctga gcaacgtttt cataggcttt tacattcaca taaggcgaac actcatcaaa
     3061 ccctttgatc gaaccaaaaa tcagacgtgg attgatttct tgaatatgct cccaggtgaa
     3121 gcccatatga tcaatggccc ctggatgaaa gttctcgact aagatatcag cttcgcggat
     3181 cagcttttcc attacctctt tgccttccgc tgttttggta tttaattcaa tagaacgttt
     3241 gttactgtta agcatggtga agtaaagcgc atcgatatca ggaatatctc gcagctggtg
     3301 gcgcgttacg tcaccaacgc cgggacgttc aattttaatg acgtcagcgc caaaccaggc
     3361 cagcatctga gtacaagatg ggccagattg cacaccagtg aaatcgagaa ctttaattcc
     3421 ttgaagtgga gttgacatag caatacctat tagttaatta aatatatgtt aatttagcgt
     3481 ttatttaagg cataatgctc tctataaata atccgcaaaa tatcgtcgta gctgcactcg
     3541 atatttttaa attcctgtat caagtgctga taatcatttc atcgccactt ccaattcctt
     3601 tctgcatggt aagtagtgag gacgaaaaga agaatatgat aaatattggt tcgcggtaat
     3661 cagagagggc cgcgacattt gtgatgttat tcttacaact gtaagaagcg ccggtgatgt
     3721 acaagtaagg gtatgattac gctttttaaa gcgtaccata aaacataaca taataaatga
     3781 tttatgatga cacgggatta ttgcggttta ctttgaagaa tataaaatgc gagtttatag
     3841 caccgcgagc gccaaataat tttatggcca caacctgtcg tattgtggcc ataaaaaatt
     3901 gtcggctgga gggagtaaaa tcctttacct caatatattg cctgtcagct ttagttcaca
     3961 aacagtgctt cgctatcgac gatgattcca tcttccgcac ctttaagcgc caggtttgcc
     4021 tcgtagtatt gctttttatc aagcagtttc tgcgcatcag caagtgcatt acgagtctgt
     4081 ttgagcggca tcagatattg gttttccatt acaccaacac cggccaggcg aagctcttcc
     4141 attgcgccct ttttatcccc tttcgccatt ttttcgttgg caatttttat cgcggcttct
     4201 ttttcgggtg tcgctacata actctcagaa attccgacag atgcattaat gacaatatat
     4261 tggtcatcat tgagattggt ctttttacct ggcttagcga actttgccca ttcggtactg
     4321 tcatctgaca acaaagctga agcttcattg gtcagttctt ttgccttttc cggatcgcca
     4381 tgaaacagag ccaggcgcgc tacctgcacg tcacgcatcg catataaacc ttgttcagag
     4441 atacgctctg ctgctgccgt tttctgggtt tgctgaactt gctgagtttg ctgttgtgcc
     4501 gctaccggtg cattatcagc agcccataca gttgaagatg ccagaattgc tgtgaccatc
     4561 gtagccataa ttaaacgttt catacttacc tcattcttta acagttttag aaaacgccgc
     4621 catcatattg ggtgagcatg aaatgagaaa tcagagagca acggtcattt tgaaagggtt
     4681 atcttacagt tgtagttatt atcgcaagaa gtattttagt cttacaattg taggaatatg
     4741 cttcctgtca cagagaacta ttcttatata ccacaaagag atgtcattta ttatatcgat
     4801 gtaaatactg attaattaaa tgtaaataca gccataacat cgtaggttct cagtgaacca
     4861 ttttcaggat aactttctat gaaagtaaac ttaatgattt ttagcttatt tttattggtc
     4921 tctattatgg catgcaatgt ttttgcattt tccatttctg gtggtagaag tgagacgagc
     4981 tataaagaga cagaaaaaac gtcagtgatg acgacaactc attctacaaa acttcagcca
     5041 tcacaggcga ttttgtttaa gttgagagaa gatgcgccac cattaaatct cacagaagac
     5101 attacgcccc ctttttcgac aaaggcgaat tatcttattc atcctgtgcg atagcgtctg
     5161 tcacctggca ggtgaacaat catgcctgat gctgatgtga acacctgccc aaatgaacca
     5221 tcctgaccca ctagcgcctg ccatacccca ccataaagcg aatcatcaag ccacaagccg
     5281 gggcaagaaa cataaatatc cccgtcacta gttcattacc gccagttaac cacatggtga
     5341 agtagtaaat gagtgcagcg aggatcacca tgccgaagaa agaagagacc gccgagacca
     5401 cgttcagtac ctgaccgatg tatccgctta cgcagacaat acacagggcg agaaacatcc
     5461 ataaataaat catagctcaa cctcctgccg cgctaaaaaa cctgtaactt cagtcacttt
     5521 aatagtaaga caatccttaa cctctggcaa tttttgctaa gcatcaatga aaacagcacg
     5581 ttaaatgcaa cgctattgcg tgcggaaaat tttttacttt gcacgattaa taatcagtag
     5641 ctgaaagcag tcagccagtg ataaagtaga gccaccgcat tcatgctcct cgtggttatg
     5701 tcctgactag tctttacact ctttacagga accattgtcg tacatgatgg cccaaccaat
     5761 tgagtgtacc gctgtcgaca tggaatcttc tgcgatacaa caattcgtat ctacagaagg
     5821 taactatgtt tccacaatgc aaattttccc gcgagtttct acatcctcgc tactggctca
     5881 catggtttgg gcttggtgta ctctggcttt gggtacagct tccttatcct gttctctgct
     5941 ttctcggcac gcgtattggc gcaatggcgc gaccattcct gaaacggcgt gaatctatcg
     6001 cccgtaaaaa cctggaactt tgtttcccac agcattctgc ggaagaacgc gagaagatga
     6061 ttgccgaaaa ctttcgttca ctcggcatgg cgctggtaga aaccggcatg gcgtggttct
     6121 ggcccgacag tcgcgtacgt aaatggtttg atgttgaagg gttggataac cttaaacgcg
     6181 cacaaatgca aaatcgcggc gtaatggttg tcggcgtcca ttttatgtcg ctggaactgg
     6241 gcggccgcgt gatgggactg tgccaaccaa tgatggctac ctatcgtcca cataataatc
     6301 agctgatgga atgggtgcag acccgtgggc gcatgcgctc taacaaagcg atgatcggca
     6361 gaaataatct gcgcggcatt gtcggtgcac tgaagaaagg tgaagcggta tggtttgctc
     6421 ccgatcagga ttatggtcgt aaaggcagct ccttcgcgcc gttctttgcg gtggaaaatg
     6481 tcgccacaac caatggtacg tatgtccttt cacgtctctc tggcgcagcc atgttaaccg
     6541 taacgatggt aagaaaagca gattacagcg gatatcgttt gtacatcacc ccagagatgg
     6601 aaggctaccc ggcagatgaa aatcaagccg ctgcctatat gaacaagatt atcgaaaaag
     6661 agatcatgcg cgcaccggag cagtacctct ggatccaccg tcgctttaaa acgcgcccgg
     6721 tgggagaatc gtcgctatac atttaacaaa ctgctcttac tctttccctc ctcaaatcag
     6781 ccataataac ctcatggttt ttatggcttt tttcttaagg ttttttcctt cgctgaatct
     6841 ctaattatta ccgtcagaaa ttaaactgtc gccgatatga gacacctgta agttactaaa
     6901 agtaaataag aaaatgcctc ttgtctcgtt tcagttttag gcgtacatta gcgccgtctg
     6961 gagcagttta gctcagaatt ctggggtgat gcccacggta aaatacagga cttttaaggt
     7021 cgttttgccc ggtgtcaact cactgtatca ggtgtaatga agtcattcag gcgtaacagt
     7081 aattacgcgg agagatgtaa agtgaaatat ttctttatgg gcatttcttt tatggtcatc
     7141 gtttgggccg gtacttttgc cctgatgatc taaaagcaaa ctgtgcaatg aaaaacagga
     7201 gccatctggc tcctgtttgc atttctggcg atggtgttta ttccgcgttt tcgtgaatat
     7261 gtttgctgct ggcgggtagt aaaccgtcgg cacggaacat cgctttaatt ccacgaatcg
     7321 cctgacgaat acggtcgcgg ttttcaatca gtgcaaagcg aacatgcgtg tcgccgtagt
     7381 cgccaaagcc aatccccggc gagacacaga ccttcgcttc gttcagcagc ttcttggcaa
     7441 attccagcga gcccatggca gcatacggtt ccgggatttt cgcccagaca tacatcgaag
     7501 ccttcggcat ttcgaccatc cagcccgctt catgcagccc ttttaccagt acatcacggc
     7561 ggcgtttgta ctgttcagca atgtcgcgca cgcactgttg atcgccctcc agcgccgcaa
     7621 tcgctgccac ctgcaacggc gtaaaggtgc cgtaatcgtg atagctttta atacgtgcca
     7681 gcgcgctgac cagcgttttg ttgccaacca taaagccgat acgccagccc gccatgttgt
     7741 agcttttcga cagcgtaaag aactcgaccg ccacatcgcg tgcacctggc acctgcatga
     7801 ttgacggcgc tttccagcca tcgtagacga tatcggcata ggccaggtca tggaccacca
     7861 gcacatcgta gcgtttcgcc agcgccacaa ccttttcgaa gaactccagc tccacgcatt
     7921 gcgcggttgg attcgacggg aagccgagga tcatcatctt cggtttcgga taactttcgc
     7981 gaatggcgcg ttccagttcg ttgaagaaat cgacaccttc caccagcggc actgagcgta
     8041 cctgcgcccc ggcaatcacc gcgccgtaaa tatgaatcgg gtagcttgga ttaggcacca
     8101 gtacagtgtc accatgatcc agcgtcgcca gcatcagatg cgccagtccc tctttcgaac
     8161 caatagtgac gatggcttct gattccgggt cgatctcaat gccgtagcga tcctgatacc
     8221 agcgggaaat ggcgcgacgt aaccgcggaa tgccgcgtga cgtggagtaa ccatgcgtgt
     8281 ccgggcgctg ggccaccgta cataattttt cgacgatatg cggcggagtc gcaccgtccg
     8341 ggttacccat actgaaatca ataatatctt cgccgcgccg acgcgcagcc attttcagtt
     8401 cagcggtaat gttaaaaaca tagggcggga gacgatcaat gcgcgtaaag cgacgttcag
     8461 ggcgagtgtc agccataatt tcctcagatt aacgttagcg cccggaccgt ccgagcgacg
     8521 ctgccacgat ggtggctgtt ttgaaaatag cctgattaat ttctgtctgt ccagcgtttt
     8581 agaacatttt tattaatgac gttaaatgag gaactttgct ttaaaaacag gaaatttatc
     8641 tgcgctaaca agaaaattac cacccatttt attaacataa taatttcaat aaatttacta
     8701 accagaagtg cattatcaag agatgcattg ctaatacacc atttatctat caatcccctt
     8761 ttaaaattgt ggtttttcct caaaaacggc ctgctggtca tcagccttca ggttacctat
     8821 catagaggtt taatccttat tcagagtcac ccgtgcacga aatattcaac atgctgctgg
     8881 cggtcttcga tcgggcagcg ttaatgctta tctgcctgtt ctttctcatc cgtatccgtc
     8941 tgtttcgcga actgttgcac aagtcggcgc actccccaaa agagttgctc gccgttaccg
     9001 ccattttctc gctgttcgcc ctgttcagca cctggtccgg cgttcctgta gaaggttcgc
     9061 tggtgaacgt acgtattatc gcggtgatgt caggcgggat tctgtttggc ccgtgggtgg
     9121 gcatcattac cggcgtgatt gcgggtattc accgttattt aattgatatc ggtggcgtga
     9181 cggcgatccc ctgctttatc accagcattc tggcgggttg tatatcgggc tggatcaacc
     9241 tgaaaatccc caaagcacag cgctggcgcg tcggtattct cggcggcatg ttgtgtgaaa
     9301 cgttgaccat gattctggtg attgtctggg caccaactac cgcattgggg atcgatatcg
     9361 tctctaaaat cgggattcca atgatcctcg gcagcgtctg tatcggcttt attgtgttgc
     9421 tggtacaaag tgttgagggc gaaaaagagg ccagcgccgc gcggcaggcc aagctggcgc
     9481 tggatatcgc caacaaaacg ctgccgctgt ttcgccatgt caatagcgag tcattacgca
     9541 aggtctgcga aattatccgc gatgacattc acgctgacgc ggtggcaatc accaataccg
     9601 atcatgtgct ggcctacgtt ggcgtgggtg aacataacta tcagaatggc gatgacttca
     9661 ttagcccgac tacccgccag gcgatgaatt atggaaaaat catcattaaa aacaatgatg
     9721 aagcccaccg cacaccagag attcattcca tgctggtgat cccattgtgg gagaaaggcg
     9781 ttgtgaccgg aacgctgaaa atttactact gccacgcgca tcagataacc tcgtcattac
     9841 aggaaatggc ggtcggtctg tcacaaatca tctccacgca actggaagtt tcgcgcgctg
     9901 agcagctgcg tgaaatggca aataaggcag agcttcgcgc cctgcaaagc aaaattaatc
     9961 cccatttcct gtttaacgct ttgaacgcta tttcatcgtc aatccgtctg aatccggata
    10021 ccgctcgcca gctgatcttt aatctgtcgc gttatctgcg ctataacatt gaattaaaag
    10081 acgatgagca aatcgatatc aaaaaagagc tgtatcaaat taaagactat attgccattg
    10141 agcaggcccg ctttggcgac aagctgacgg ttatctatga tattgatgaa gaggtgaatt
    10201 gctgcattcc cagcctgctg atccagccgt tggtggaaaa cgccattgtc cacggtatcc
    10261 agccttgcaa aggtaaaggc gttgtcacca tcagcgttgc agagtgcggt aatcgggtac
    10321 gcattgcggt gcgggatacc gggcacggca tcgatccaaa ggtgattgag cgggtcgaag
    10381 cgaacgaaat gccgggcaat aaaattggcc tgctgaatgt ccatcatcgc gtgaagttat
    10441 tgtatggcga ggggctgcat atccgccgcc tggaaccagg aacggaaatt gcgttttaca
    10501 ttcctaacca acgcacccct gtcgcctcac aggctacgtt attgctttga gccggagtga
    10561 tattgtgaaa gtcatcattg ttgaagacga attcctggca caacaggaac tgagctggct
    10621 aattaaagag cacagccaga tggagattgt cggcaccttt gacgacggtc tggacgtgtt
    10681 gaagtttttg cagcataacc gcgtcgacgc catttttctg gatatcaata ttccgtcgct
    10741 ggatggtgtg ttactggcac aaaacatcag ccagttcgcc cataaaccgt ttattgtgtt
    10801 tatcaccgcg tggaaagaac atgcggtaga agcgtttgaa ctggaggcgt ttgactacat
    10861 tctcaaaccg tatcaggagt cacgcattac cgggatgctg caaaaactgg aagcagcctg
    10921 gcagcaacag cagaccagca gtacgcctgc cgcgacggta acgcgtgaga atgacaccat
    10981 taatctggtg aaagatgagc gaatcatcgt cacgccaatt aacgatatct attacgccga
    11041 agcgcacgag aaaatgacct ttgtctatac gcggcgtgaa tcctacgtaa tgccgatgaa
    11101 cattaccgaa ttttgcagca aactgccgcc atcgcatttt ttccgctgcc atcgctcatt
    11161 ttgtgtcaat ctgaacaaaa tccgcgagat cgaaccgtgg tttaataaca cctatattct
    11221 gcggctgaaa gatctggatt ttgaagtgcc ggtcagccgc agcaaagtta aagaattccg
    11281 ccagttaatg catctttaat cgggaacttt catgaaagca ccaggcttgc ccgccgatca
    11341 acaatttttc gccgatctgt tcagcggcct ggtgcttaac ccgcaactac tggggcgcgt
    11401 ctggtttgcc agccagcctg cctcgttacc ggtgggcagt ttatgtattg attttccccg
    11461 tctggatatc gtgctgcgcg gcgaatacgg caatctgctg gaagcaaagc agcaacgtat
    11521 ggttgaagga gaaatgctgt ttattccggc gcgcgcggct aatttaccga tcaacaacaa
    11581 accggtgatg ctgttaagcc tggtgttcgc accgacctgg cttgggttat cgttttacga
    11641 tagccgcacc acgtcgttgt tgcatccggt tcgccagatc caacttccca gcctgcaacg
    11701 cggcgaaggt gaagcaatgc ttactgccct cacccatctt agccgttcgc cgctggagca
    11761 gaatatcatt cagccgctgg tgttaagttt gctgcatctt tgccgtaacg tggtgaatat
    11821 gccgccgggc aattcgcagc cgcgcggcga ttttctctat cacagcattt gtaactgggt
    11881 tcaggataat tatgcccagc cgctcacccg tgagagcgtg gcgcagtttt ttaatatcac
    11941 gccaaatcat ctgtcaaaac tgtttgctca gcatggaaca atgcgtttta tcgagtatgt
    12001 tcgctgggta cgaatggcaa aagcgaggat gattttgcaa aaatatcatc tgtcgattca
    12061 tgaagtggcg cagcgttgcg ggtttccgga tagcgactat ttttgtcgcg ttttccggcg
    12121 tcagtttggt ctgacgccgg gagagtacag cgcccgtttt cagggctaac gtcagaaggt
    12181 taattctgtt tccagcagcg tcaggatgct ttgtgcatct tgcgcggcaa acaacgactg
    12241 gcggaagttt ttattcacca gtttacgcgc cagctgcgag aagactttca catgattcat
    12301 cccttcgtta gcgcccagtg tcagcatgat caccagttcg acttcgccca tttctgactg
    12361 ccagtcaacc ggtttcacca gtcgggcaat gctgatactg gaatgacgga tccactgaga
    12421 tttggtgtgc gggatcgcta cgccaaaacc aacgccggtg gtaacaatct cttcccgctg
    12481 ccagacatcc tcttccagct cgaacggatg ttcagtgcgc ccgttaacgc cgaggttacc
    12541 gcacaggaac tggatcgcct gctctttatt gctaaaagcc tgatcaacaa agatattctc
    12601 cagcgccagc agagggcgaa cgtcttcttc cggcgtaaac gcggtgagta acgcttcaat
    12661 ctcctgcgcg ctgcggcatt cacatgcctg acgcgccagt tcccgacacg cctcgctatc
    12721 cagttgacga agctggcttt tcaccgccgg aatgcgcggg ctactcatac tcagctcgtc
    12781 caggcccagc ccaagcagta gcggcagata acggctttca ccgcccagtt caccgcaaat
    12841 acctacccat ttaccccgct gatgcgcagt ggtaacaatc tgctgcaaca tgcgcaggaa
    12901 cgatggcgta atcgggttat acagcggcga tacgcgcggg ttattacgat cgaccgcata
    12961 cagatactgg gtcatatcgt tggagccgat actgaagaaa tcgacctcat cgcagaagtg
    13021 atcgatgatg tagcacaccg acggcacttc caccatgata cccagcgtaa tcgtctctgc
    13081 atgacgcagg ccatcgcgct taagctcaac gatcgctttt tggatctcgc ccttcaccca
    13141 taagatctga tcgaggctgt gaaccattgg gatcatcaac tgggcgttgc cgaaactggc
    13201 ggcgcgcaaa atggcccgca gttgagtgcg gaacaggcca gcaaattccg ggtaaattcg
    13261 taccgcacga tagccgagga acgggttctc ttcctgggga atgttgaggt aaggaatgct
    13321 tttatcgccg ccgatgtcca ttgtgcggaa gataatcggc ttgtcgcccg cggccagtag
    13381 cacctgctgg taggcttcaa attgctcctg ctcgtccggc gcactgtcgc gatccatata
    13441 gagcatttcg gtacgaaaca gcccgacgcc ttccgcgccg ttggcaaacg cgccaggcgc
    13501 ttccagcgcg gtgccaatat tcgccgcgat atcaatgcgc ttgttatcac gggaataggc
    13561 cagctgcgcg gcggcctgcg cctgctgctg ctgacgttta tccgccagca tctgcgcaac
    13621 ctgataataa ccgctcaccg cgtcattcgg gttaatcgcc aacacgccgc actgggcgtc
    13681 aagcaccgcc ggttgcccgg catagcgcgc aatggcatcc agcggcaggc cgctcagtac
    13741 cgggatcgcc gaggcgcggg ccagaatcag ggtatgcgag gtgcgcccgg ttttctccag
    13801 aatcatgccc gcaagatttt ttaaattgag gctcaaaaac tggctagggg ttaaatcctc
    13861 tgccaccaga atggtcggtt tttccagcac cagattgttg cgcggtttta gttccggcca
    13921 ggtgatatgc agtaactgtt cgctgatatc gcgaatgtcg ctaacccgct cgcgcagata
    13981 atcactggca gaagcagaaa gtttggcgca aacctgctcc atattgctga tgatcgccgc
    14041 ccccagcccc tgatgctgtt ctgccatcag gtgacggata ttgcctgcaa attcatcatc
    14101 ctgaatcagc gacaaatggg cgctgaggat agttttgctt tcgccgtcac gctcacgcaa
    14161 ttgctggttc agttgctcgg caagcgttgc caggctgtgc tccagccggg tggaatcttg
    14221 cgcactggcg gggatcgccc ggtaactgtc gaggctgtcg ctctgtaaca gggtcagcgt
    14281 acccacgccg acgccgcttg ccagcacatt gccgtacagt aaatcaggat tcagacggct
    14341 taatgaacgc ggcagcggat gcgccgtcag ttctgcctgc gttggctgga cgctgtcgct
    14401 gtcgataaag cgcacctgga tgtactcttc cagcacgcgc cgcgcctgct cttcatcgct
    14461 gccgctaatg ttcaggctgc aaccgtcatt aaatagggtg ccggtgccaa tcagcgccag
    14521 cgagcttttg gcatctgcct ttgcgttctg gcgatggtta ataaaagtga tttcgctttg
    14581 ccactggctg cactgttctt taagttccca cgccggacga gcatgcagac cgtttggcaa
    14641 aggacagaga aattgaatcg ttaacataac cgctccgggt taatcaggag atcatctgaa
    14701 atccgtcagt tgaacaaccg tctcacgcgt aagacgttga ataagggcag ataaaagctg
    14761 ctgcatctgc aaaatgtcac ggcaatcggc aatcgatgcg gcgcaatgtc cgtggcgggt
    14821 ggcaggcccc atcaccactg tgggtacgcc agtgccggtt aagtgcaccg ccccaccgtc
    14881 cgtgccaccg ttgctgaaca tatccgcctg caacggcacg ccaatttctg ccgccacggt
    14941 ttcaatccac gcggtaagtt ttggcggcgc aatcagcgat ttatcgctta acaccagcat
    15001 cggcccgtta ccaatctgac gatggttagc cgcgccataa tcaaagtttt tcgcccagca
    15061 ggcggtatca agcacaatgg cgacgtccgg cgacaccgcg cgggtggcag tttgcccgcc
    15121 gcgtaatccc acctcttcgc tggaacttgc caccagccac acctccgcag gcagttcggc
    15181 gtcgtgcagc tcacgtagta acgtcaccag cagatagcaa ccgaggcgat catcaaaggc
    15241 tttccccatc acccgctggt gagggagaac ctgaaaagtt gtatcaaagg tgacgcgatc
    15301 gcctggacga attcccgcct gcatcacttc gtcatacgag cgcgcaccaa tgtccacgcg
    15361 tatggtgctg acgtcattcc cctgccggtc gccgtcaagc aggcctggaa ttttgcactc
    15421 ttcacgggta gtgatacgca ccgactgaag ctggcgggca gccatgcgta cattgccaac
    15481 cggcaacaca tcgatcgccc cttcgcggga gatgctgcgc accataaatc ccacttcgtc
    15541 catatgcgca cagatcatca ccttcggacc tgtcgactca ttgagtcgga tcagcaccga
    15601 tcccagccca tcaaatcgca cttctttttg caggcgatcc gcttcttcca gcaggatctg
    15661 ccgtacttcc tgttccgagg aggcgatcgc atctgcctcg ctcaacgctt ttaataacga
    15721 taaatccatt atgcctctcc cgtgagcaac actgttttcg gcatggcgta gagcacttct
    15781 acgccttgcg gggtgaccag cacaacatct tcgatgcgca cgcccccttg ccctggcaaa
    15841 taaatccccg gctccacggt cagtaacatg cctggctgta gcgtcgtggt gtcccgcggt
    15901 gaaaaacgcg gatcctcatg gacttcaata ccgatagcat gaccggtgtt atgaccgaaa
    15961 tagtcgccat aaccggcttc tgtaatcacc cggcgcgcgg cgtcgtcaac ctgctggcag
    16021 cgtacgccgg ggcgaatcgc ggagattgct gcgagctgtg cctgcaggac aatctgatag
    16081 acgttaaaca gcgggtggga ttcggcactt accccttcgc cattcaccag caaggtgcgc
    16141 gtcatatcag agcagtagcc ctgatacagc gcaccgaaat cgagagtgac aaactcgccc
    16201 gctgcaacaa tcttgtcgct ggctttgccg tgcggcagcg ccccacgcca gccgctggcg
    16261 acaatggtat cgaaagaggc tttttctgcg ccctgctggc gcataaacca ctccagttca
    16321 gcggctatct cgcgctcgct catccccgcc tgaataaagc ggcgaatatg ctctgcaccg
    16381 cgatcggcaa tcccacaggc gagacggatt ttctccacct cctctggcgt tttgagttgc
    16441 cgcagcacat ccggcgtggc gctaaccagt ttcgcattga gttcagactg ccagcggtgc
    16501 gcggtttccc aactcacctg ctggccttca aaaccgagcg tttgcaactg ctcatcggca
    16561 aagatttgcc tgacgatggt ggcaagcgtg tgcatcgcgt cgagcaaatg cagctggtaa
    16621 ccttgcgtgc gggcttctac atccgcgtaa tagcgcgagt ccaccagaat gtgcgcactt
    16681 tcacggcaaa tcaccacata gcctgatccg gtggagatcc ccagatgcgg ctgtttgttc
    16741 tgccgtgagg agagaagcac cgcatccagt tgttgcgcct taagccagtc gcgcagcgaa
    16801 gcgagtaatg tcatcaggat tgtccttctt acaggctatc gattaacaat ttgcctttac
    16861 ggaacatcat cagacgcagg aacaccacca tcagcgcagt tatgactgcc cccagcgcga
    16921 tccccgccat gtagacgccg aggttagtga ccagcggcca tgcccagata gcggactccg
    16981 ggaaccattg cactgcgccc agccagacag cggcggttga gccgacaatc gcgccgacca
    17041 tataggacgg aatggcggtg atggggcttt ccagcgcaaa cggaatcgca ccctcactga
    17101 tccccataaa cgccaggaac atggcggttt taccctgcgg ataaagctga gcactgaaca
    17161 gacgtttgcc ggttaaacgg cggtcgataa tggtcgccag acccagacca atcggcggaa
    17221 taacaatagc gatggaacgc gcggtaaccg gtaatacgtg gtcagtggta aagctgaagg
    17281 caacgaaacc agctgctttg ttgatcgggc caccaaggtc gattgctgtc gccgcggcga
    17341 tccccatcgc gtacattagc gcacccttct cacctgcggc agtcagcacg gtacggatac
    17401 cgccgttgat ccagccacca aagggggtga tgacgtagta catcgccagc atgacaaaaa
    17461 tggcggaaag aatcggtagc aaaaatgtgg ttttgaacgc taacaggaaa tccggtaact
    17521 ggattttctg gttcatccac ttcacaaggt aaccagcaac gatagaaata atcagggcac
    17581 caatgaaggt ggacggcacc ggcgaagagg tcgcccattg catcgtgctg ggatcgaagt
    17641 tcagcagttg cgtcggctgg gtagacatca acccaccgat aaaaccagcc gggaacgcca
    17701 gtttgccacc gatagagttc gccacaaatg cagcaaacat cggaatggca aaaccaaaca
    17761 acacgccgcc gaaggactgc gacaaccagg caaatttcag taaggagagg tcgaaaccgg
    17821 agaatttccc gctattaaga gcatccatga tgccgatatc agcaggaatt ttaagccagc
    17881 tataagcaat cagctggctg aaagcgagga tcacaccgcc cataattaag gtcggcacca
    17941 tgcgtgaaat ccctgacatc acatgctgcg ggagttcacc ccagaagctg gttttagagg
    18001 cctgcggatt cttaactgcc gctgccgcac cggatgcgcc aggcacaacg gttgcactgc
    18061 gttttttaat ggccataaca attatccctg tcgaatatta ctgttgttca gaggcaatca
    18121 tctcttcgat ttctttgatg atgcccgcag cgtttttaat tgcgtcctgc aaagtgattt
    18181 cataaacgtc gcgtgattcg aaacgttcgt tatcttccgg ggtaaccgcc acggagtgga
    18241 tgatgatggt cgcttcggcg atatcctgcg cggtcaggcg attctggatg ccgtccgcgc
    18301 cctgagtttc aattttcact tcatagcctg cttccaccgc cgcttcttcc agcgcctggg
    18361 ctgccataaa ggtgtgagcc agacccatcg ggcaggcaca taaagcaatc agtttcttac
    18421 tcatcgttaa ttcctcgttc agtgatagtt gacgagtgaa attatgaacg tgcaaaacaa
    18481 atcgccgtta ccagacaaat aatgcattta ctggagaatt aatcccgctg tacggaagtg
    18541 tgacgcaggt cggccttgtg aggggacaca aaggaaaatt gaattaactg tatgatttaa
    18601 aagattatcg ggagagttac ctcccgatat aacaggaagg atttagagaa tgtgacctaa
    18661 ggtctggcgt aaatgtgcac cggaaccaag aaggcccgga ttgtcatgga cgatgagata
    18721 caccggaata tcatggacat attctttaaa gcgcccttta tcttcaaatg cggcacggaa
    18781 accagaggct ttgaagaact caaggaagcg cggcacgata ccacccgcaa taaacacgcc
    18841 gccaaatgtc ccgagattga gcgccagatt gccgccaaaa cggcccataa tgacgcaaaa
    18901 cagcgacaac gcgcggcggc aatcggtgca gctgtcagcc agcgcgcgtt cggtaatatc
    18961 ttttggcttg agattttctg gcaggcggtt gtcggcttta acaattgcgc gatacaaatt
    19021 caccagccca gggccagaaa gcacgcgctc tgccgaaaca tgaccaattt ctgcacgcag
    19081 tatttcgagg ataatgccct cttcttcact attcggcgca aaatcaacgt gaccgccttc
    19141 gcctggcaag cttacccaac gcttatcgac atggaccaga tgcgcaaccc ctagccccgt
    19201 tccggcaccg taaaccgcaa taggcttacc ttctaccggt tctgcgccac caaactgaat
    19261 cagatgctct tttttcagca tcgggatcgc catcgataca gcggtaaaat cgttgataat
    19321 ttccagatgg ctaaaaccga gattcttttt catttcggca attgagaacg cccaggtatg
    19381 gttggtcatt gccacccagt caccggtaat tgggcaagcg atggcaatac agccgtcttg
    19441 cacctcgatc ttatgttctt caagataaac gcgaatgacc gcttcgaggc tggggtaatc
    19501 aagccctgaa taggtcttag cctgcgagat ttcaccactg gcaatatcac acagagcaag
    19561 acgtgcgttg gtgccgccca catcaccgac taatgcatac tttgtcattc ttcaactgct
    19621 ccgctaaagt caaaataatt ctttctcaca ctgtaaatac ctgggggcat aacaacaacg
    19681 ccggaaaggc aggctccctg taaatatcga tctgggtcac acaattactt tatcgtttca
    19741 gcaccaattg cagcgatgcc tttttgcaag ctgggcaaac taagtatctg accccgcata
    19801 aggaatagaa catgctccat ccgcgagcca gaaccatgtt gttattatcg ctccccgccg
    19861 tggcaattgg gattgcgtcc agtcttattc tgattgtggt gatgaaaatc gcctcggtat
    19921 tacagaattt gctctggcaa cgactgccgg gaactctggg gatagcccag gattcacctc
    19981 tctggatcat cggtgtatta acgctaacgg gtattgcagt ggggttggtt atccgtttca
    20041 gccagggtca tgccggacca gaccccgcct gtgaaccgct gatcggcgca ccagttccgc
    20101 cctctgcgct accaggactt atcgtagcat taattcttgg tcttgctggt ggcgtcagcc
    20161 tggggccaga acatccgatc atgaccgtca atatcgccct tgcggttgcc attggcgctc
    20221 gtctgttacc gcgcgtcaac cgaatggagt ggactatttt agcctctgcc ggaaccatcg
    20281 gcgcgctgtt tggcacacct gttgcggcgg cgttgatatt ttcgcaaacc ttaaatggca
    20341 gtagtgaagt tccgctatgg gatcgtcttt ttgcgccgtt aatggcggca gcagctggtg
    20401 cactaactac cggattattt ttccatcctc atttttcact gcccattgcg cattacggac
    20461 agatgcaaat gaccgatatt ctcagtggtg caattgtcgc ggcgattgcc atcgcagcag
    20521 ggatggtcgc cgtatggtgc ttaccacgat tgcacgcgat gatgcatcaa atgaaaaatc
    20581 cggtgctcgt gctgggtatt ggcggattta ttctcggtat tctgggggtt atgggtggac
    20641 cagtttcgct gtttaaaggg ctggatgaga tgcagcagat ggtggcaaat caggctttca
    20701 gcaccagcga ttactttttg ctggcggtga ttaaacttgc cgccctggtc gttgctgccg
    20761 ccagtggctt tcgcggtggg cgaatcttcc cggcagtgtt tgtcggcgtg gcattagggt
    20821 tgatgctgca tgagcacgtc cccgccgttc cagcggcaat aaccgtttcc tgcgctattc
    20881 tcggcatcgt gctggtggta acacgcgatg gctggttaag tctttttatg gcggcagtcg
    20941 ttgtacccaa taccacattg ctaccgctgc tctgtatcgt catgcttccg gcatggctgt
    21001 tattagcagg taagccgatg atgatggtca atcgtccgaa gcaacagccc ccccacgata
    21061 acgtttagca aaaaatgctc tcctttatga tttaagagtt atggcgatga tacggggctt
    21121 tacctccccg taatattgcg ttaacaggcc gctgacggct tcgatcgtga aggagaataa
    21181 caatgttcag gtcactgttt ctggcggccg ccctgatggc atttaccccg cttgcggcaa
    21241 acgcaggtga aatcacccta ctgccatcaa tcaaattaca aattggcgat cgcgatcatt
    21301 acggtaatta ctgggacggt ggtcactggc gcgaccgtga ctactggcat cgcaattatg
    21361 aatggcgtaa aaaccgctgg tggcgtcatg ataatggcta ccaccgtggc tgggataagc
    21421 gtaaagcgta tgagcgtggc tatcgtgaag gctggcgcga tcgtgacgat catcgcggaa
    21481 aaggccgcgg acatggacac cgccattaaa cacattcgcc aatggagcac aatgcctgat
    21541 gcgacgctag tgcgtcttat caggcctaca aaccggctct ttaacgcgta gggcggataa
    21601 ggcgtttacg ccgcatccgc caatagtgcc agatgcgacg ctcattcaac tacaatccca
    21661 gcgccgtccc caccaacaac cagatattca gcgccacgac cagcactaca atcacccagc
    21721 ctgtctgttt tacgcgtttg ctgttcacca gatcgcccat caacttgctg tcactggtga
    21781 aaatcagcag tggaaccagc gccagagcga taccaaaact taacagcacc tgactcataa
    21841 ccagaatccg tgtcggatct aatcccatca gaatgacaat aaatgacggc aacatggtaa
    21901 cggtgcgacg cacccacagc gggatatgga agcgaatgaa gccctgcatc accacctgcc
    21961 ccgccagtgt ccccaccacc gttgatgaca gtccggcagc aaccaggctt aatccaaaga
    22021 ccgttgccgc agcatggctt aacaacggtt gcagcgtcag gtaagcctca tcaagatcgg
    22081 caacgccagt atgaccggaa aagtggaacg ccgccgcagc cgtagccatc atcgccagat
    22141 tgacaaaacc ggcaatagtc atagcgatag ccacatccca tttggtggca gaataacgtt
    22201 gttgacgcga accaccatgt aaatgctgag tcagcgaaga gtgtaaataa atcacgtgtg
    22261 gcataatcgt cgcccccaac acacctgccg ccaggaagac tgcctccgag gtgggtaaac
    22321 tcgggattac cattccttta cccagttgcg ccaggttagg ctgggagaaa atcaactcga
    22381 caatgtaagc cgcagcaaca aacagcagta acccgccaat cactttctcc agcggttttt
    22441 gccctcgacg ttgcagcatt aaaatcagga aagtcgcgat ccccgtcagc accgcgccct
    22501 gcaacagcga aacacccaga atgagtttaa aaccgatcgc cgcaccaata aattccgcca
    22561 ggtcggttgc catcgcaatg atttctgcct gaacccaata gaaccacact acgggacgcg
    22621 gatagtgatc gcgaatctgc tccgccaggt ttttaccggt ggcaatcccc agtttggcag
    22681 acagtatctg aatcagcatc gccatcaggt tggcccaaac gacaacccac agtagctgat
    22741 agccgaagct ggcacccgcc tgaatattgg tcgcaaagtt accaggatcg atataaccaa
    22801 tcgccgcaat gaacgcaggt cccattaatg cgagcctcat cttgcgcgcc gcccgtccgc
    22861 tgctactctc aacgcgatag ttagtcatct tgtgcctcta aaacatagcc tttgctatgt
    22921 ttcatgctat gtcaaacgag aatgattatc aaattcattt aagtggattg tgatgatttc
    22981 tttgatagac cagaattatg acgacgaaaa gattgatgac ttgaatgttg tgattatgtt
    23041 taatgttagc acatttacat aactttcagc ttccatacac aacatagcag aaatgtatga
    23101 cagctcacta tttttgaagc ttgtcacagg acgtcattat agtgtgtgtc agatctcgtt
    23161 ttccttaacc atgttacata gaatgtgcac ggaaatttaa cctgcctcat atttggagca
    23221 aatatggacc gcgtccttca ttttgtactg gcacttgccg ttgttgcgat tctcgcactg
    23281 ctggtaagca gcgaccgcaa aaaaattcgt atccgttatg ttattcaact gcttgttatc
    23341 gaagtgttac tggcgtggtt cttcctgaac tccgacgttg gtttgggctt cgtgaaaggc
    23401 ttctccgaaa tgtttgagaa actgctcgga tttgccaacg aagggactaa cttcgtcttt
    23461 ggtagcatga atgatcaagg cctggcattc ttcttcctga aagtgctgtg cccaatcgtc
    23521 tttatctctg cgctgatcgg tattctccag cacattcgcg tgttgccggt gattatccgc
    23581 gcaattggtt tcctgctctc caaagtcaac ggtatgggca aactggaatc ctttaacgcc
    23641 gtcagctccc tgattctggg tcagtctgaa aactttattg cctataaaga tatccttggc
    23701 aaaatctccc gtaaccgtat gtacaccatg gcagcaacgg cgatgtccac cgtgtcgatg
    23761 tccatcgttg gtgcatacat gaccatgctg gaaccgaaat acgtcgttgc agcgctggta
    23821 ctgaacatgt tcagcacctt tatcgtgctg tcgctgatca acccttaccg tgttgatgcc
    23881 agtgaagaaa acattcagat gtccaacctg cacgaaggtc agagcttctt cgaaatgctg
    23941 ggtgaataca ttctggcagg tttcaaagtt gccattatcg ttgccgcaat gctgatcggc
    24001 tttatcgccc tgatcgccgc gctgaacgca ctgtttgcta ccgtgactgg ctggtttggc
    24061 tacagcatct ccttccaggg catcctgggc tacatcttct atccgattgc atgggtgatg
    24121 ggtgttcctt ccagtgaagc actgcaagtg ggcagtatca tggcgaccaa actggtttcc
    24181 aacgagttcg ttgcgatgat ggatctgcag aaaattgctt ccacgctctc tccgcgtgct
    24241 gaaggcatca tctctgtgtt cctggtttcc ttcgctaact tctcatcaat cgggattatc
    24301 gcaggtgcag ttaaaggcct gaatgaagag caaggtaacg tggtttctcg cttcggtctg
    24361 aaactggttt acggctctac cctggtgagt gtgctgtctg cgtcaatcgc agcactggtg
    24421 ctgtaagacc atacataaaa aagccgggga taattccccg gttttttacg cctgttaatc
    24481 agctaatggc tgcgggcgac caatcaaata cccttgcaga tattgcaccc cgagcttatg
    24541 caatagcgcc tgctgctgtt gcgtctcgac aaactccgcg accacactca atgacttcgc
    24601 tttcgccaga tcggtaattg atctcacaat catcgcatcc agcgtgttcg tgacgatatc
    24661 tttcacaaag acgccatcaa ttttgatgat atcagcctgc aaacgcttta accgttcgta
    24721 gttggcatat ccggtgccaa agtcatcaat cgcaatccgg aaaccaaact tatgcagctg
    24781 ctcgatgttg tacatgctgc tttctgcgtt agaaaacgcc tgctcctcgg tgatctcaag
    24841 aatgaccgcc tgcggggaga tgtgataacg cttaaacaga cgaataatcc gcccggcaat
    24901 attcttttgc agcagcgtga gcggcattaa attgactgaa aagcgcgggc cttttttgtc
    24961 gcaaggatgt gttgccagcc acttcaacag ggattccagc acttgcaaat caaaacgcgc
    25021 gctaaggtta aactgagcaa taaggggcag aaacttatcc ggggtcataa tgccgccgtc
    25081 atatttcagt cgcgcgagga tctcatcata accttcaccc tctttgttgc gaattggctg
    25141 ggcgtagagc agcaaatcac cctgatctaa cgccgtacga atggtattca gcaatagcac
    25201 ctgtttagtg gtctgcccgg aaaccatctc ctctctgcta tccagcgcca gcacatggtg
    25261 atgtgcgcag gattgctccg ccagccagct taactgcccc aacaagggtt gcagggtttc
    25321 ctgatttcca tcaaaacgcc cccaggcagc accatagccc atatccagcc cggtattgtt
    25381 ccagtgaatt tgccgactat tcaggatgtt aaccatatgc tggagtcgcc cttccgtttc
    25441 cggtccactc agcactaaca gcagttcact acccggcaat tgatacaact tttcgttttc
    25501 ctgcatcaac ggcagcagcg tacggtaaat tgatcggata cagtgaacgc gcatcattaa
    25561 gccgtaatga cgactcataa actcaagatt atcaatgcgc aggcagcaaa aactcttgcc
    25621 cgcctcttgc tccggcgctt gttctaacgc acgaaagttg ggtaggagcg ttaacggatc
    25681 ggtcagcgcc tgcaaatgcc agcggcgatt aagccattca ctgcgatgat aaatgcgcac
    25741 catatagagc aggcaaacgc taaaggaaat cagcaccgcg agaataaatg ccagcgaata
    25801 ttcggttagt accccttgca aaaagttctg gttgtaattc agcaggcaaa gcgttgaaac
    25861 agcccaggtg agatttaaaa acggatagcg aagcttaccg acaccgaggg taaagatgat
    25921 aaagaaaaca ggtaccagat aaccagcaat aaagtcattt tcatacggtg tgcacatcag
    25981 aagtagcagc acgctaagag ctgccagcca gcttaaggta aatgcgcgtt tctctttgct
    26041 caacgacgga gcgatatccc tacgccacaa tatctgtgca aagtggggac ttacaatcat
    26101 gcgggcgaga tagtaaaaaa gcatgttgta aatcagcact gcggtgaaaa ggcttagcaa
    26161 atcaacgacc gtgaaaatgg catccgcatc gccgaaaaag gtagatatct ttagcggaaa
    26221 atcaaagaaa tttcccacaa gatacatgct gcatttgatg ccaatcggcg ccaccaaacc
    26281 aagccagaaa agacgttgcc agacatatcg gctggtcaat ccataacgcc aacgcgtccc
    26341 cagctgccag cgtaatattg cgcaggcaca caacaccaca aacgtctggc tgccgagcat
    26401 gaccgcggtc tgtaaaaaag agagatgaaa gttccacaga ttggtgcaaa acatcccgca
    26461 taaaacgggg acgacaccgc gccagccaaa gataaacaag attgacagca ttacgcacag
    26521 tggcatccac gccagaaaga tataactgga gtggataatg gcaagtggcg aaataaaacg
    26581 ggatagctga ataagtacca cggtgagcgt aaacgctagt gtgaatatct tgatattttt
    26641 tatcaggtta tgctccacaa acatgagtac actactctca ctgatcgggg ttttcaggca
    26701 cacaaataat aagtttattc gcatcgcgaa gcaaacaggg gccagcctgt tgccgatttt
    26761 tcatccaatg atgctttcac caggcggcgc taacccggac gtagactgta ttacaaaagc
    26821 ggcaaaaagc agagacaaaa aacccccgct ttacagcgag ggtttgaaat ttggtggagc
    26881 taagcgggat cgaaccgctg acctcttgca tgccatgcaa gcgctctccc agctgagcta
    26941 tagccccacg atgcgtttac gtaccaaatt tgctgggtgc aaaatttggt ggagctaagc
    27001 gggatcgaac cgctgacctc ttgcatgcca tgcaagcgct ctcccagctg agctatagcc
    27061 ccatcacgta aagcttgtcg agttgacggg cggcatcata tgaattccgc ccgaatgtgt
    27121 caacggcaaa ttgccacgtg tagtttcaat cgctgaaaaa tcaggcaaat gaacaatttt
    27181 ggaaaaccgc tcgcattcag tagttattca tgtcacggtt tcctgtaaag tggtgttata
    27241 aaatgaacta ctaatagacc cacatacatt cagggaattg ttatgttcaa ggagaggatg
    27301 acgccagatg aacttgccag actgaccggc tatagccgcc agaccattaa taaatgggta
    27361 cgcaaggaag gctggacaac gtcaccaaaa ccaggcgtcc agggtggcaa agctcgactg
    27421 gttcacgtca atgaacaggt tcgtgaatat attcgcaatg ctgaacgtcc agaaggtcag
    27481 ggagaagcgc cagccctttc tggtgatgcg ccgcttgaag tgttgctggt gacactggcg
    27541 aaagagatga cgccagttga acaaaaacag tttacatcct tgcttctgcg ggaagggatt
    27601 atcggattgt tacaacgctt agggattcgc gatagcaaat aatatgaaaa gattacgcaa
    27661 taaaatgacc accgaagaac tggctgaatg cctcggtgtg gctaaacaaa ctgttaaccg
    27721 ttggatcaga gaaaaaggct ggaaaacgga aaaatttccc ggcgtgaaag gcggtcgtgc
    27781 cagacttatt ctggtcgata cgcaagtttg cgagtttatt cagaacacgc cagccttcca
    27841 taacacaccg atgcttctgg aagccgaaga accccttgcc gaatacgcac cgggtattcg
    27901 cactcctgct tatcggcaaa tcatcagcgc aatagacaac atgactcaca gtgagcagga
    27961 aaaagttgcg caatttttgt cacgcgaagg aattcgtaat ttccttgctc gtctcgacat
    28021 agatgaatca gaataaataa aaaacggcag gggtatctct cctgccgttt atcttttatg
    28081 cgctaattat tgctgatttt cgcgttcagc aataaaagcc agcgctttgt tgatacgctc
    28141 gatactgcgg gtcttaccga ttgcgtgaac ggtaacatcc agcgccggag actgccccgc
    28201 accggttacg gcgacacgca gcggcatacc aactttaccc atacccactt ccagctcatc
    28261 cgccgtcgcc tgaatagcgt gatgaacgtt ttcagcggtc cagtcagtaa tcgcagtcag
    28321 tttgtcacga accacttcca gcggctgacg cgctaccgga cgcagatgtt ttttcgcggc
    28381 gtcggcatcg aactcagcaa aatcttcgta gaaataacgg cagctctgtg ccatctcttt
    28441 cagcgtcttg cagcgttcgc ccagcagttt caccagatca gccagctgcg ggccgttacg
    28501 ggtatcgata ttttcctgct caatgtgcca ttgtaagtga gtagcaacat actccggcgg
    28561 cagcgcgtta atgtagtgat ggttcagcca cagcagcttg tcggtgttga acgcactggc
    28621 agatttgctg acggcattca gagtgaagta tttgatcatc tcttcacgag tgaagatttc
    28681 ctgatcgccg tgggaccagc ccagacgcac cagatagttc agcagtgctt ctggcaaata
    28741 accgtcatca cgatactgca ttacgctgac cgccccgtga cgtttggaca gttttttacc
    28801 gtcatcgccg ttgatcatag aaacgtgcgc gtaaaccggt accggcgctt tcagggcttt
    28861 aagaatgttg atctggcgcg gcgtgttgtt gatatggtct tcgccacgga taacatgggt
    28921 gatttccata tcccagtcat caacaaccac acagaagtta taggttgggg aaccatcggt
    28981 acggcgaatg atcagatcat ccagctcctg gttgctgaac tcaatcgggc cacggatctg
    29041 atcgtcaaaa acaacagaac cttcctgcgg gttagcaaaa cgtacaacgc acggttcatc
    29101 atcagcatga tgctcatggc tgtggcggca gcgaccgtca taacgcggct tctcaccttt
    29161 cgccatttgc tcttcgcgca gcgcttccag gcgctcttta gaacaatagc atttataagc
    29221 tgtgccctct tccagcatct gatcgatcac cgcgttgtag cgatcaaaac gtttggtctg
    29281 gtagtacgga ccttcatccc actccaggct cagccagttc atgccatcca taatggcttc
    29341 gatagcttcc ggcgtggaac gctcaagatc ggtgtcttca atacgcagca cgaactcacc
    29401 gccgtggtta cgtgcaaaaa gccaggagta aagagcagta cgcgcgccgc caacgtgcag
    29461 atagcctgtt gggcttggcg cgaagcgagt tttgattttc atgaaatggc cttacgttta
    29521 gaaagatgcc gacaaccggc aaatcctgga aaaattgagt gggcgatatt ctatcactcc
    29581 cgcctgattc ctcaatgtag ttcgggttgc aaccttacgc attattgttt tcgttgacaa
    29641 attgcgcaac acggtctgtt ttgcgatcat cttgcttaaa tttacgacga acgaacaatt
    29701 tctttagaaa atgcgttgac tcattttgaa ctctccctat aatgcgactc cacacagcgg
    29761 gggtgattag ctcagctggg agagcacctc ccttacaagg agggggtcgg cggttcgatc
    29821 ccgtcatcac ccaccaacta ctttatgtag tctccgccgt gtagcaagaa attgagaagt
    29881 gggtgattag ctcagctggg agagcacctc ccttacaagg agggggtcgg cggttcgatc
    29941 ccgtcatcac ccaccacttt ctcgccagct agatttcttg taacaatgtg aagtaccgaa
    30001 gtgggtgatt agctcagctg ggagagcacc tcccttacaa ggagggggtc ggcggttcga
    30061 tcccgtcatc acccaccact tcgggtcgtt agctcagttg gtagagcagt tgacttttaa
    30121 tcaattggtc gcaggttcga atcctgcacg acccaccaat gtaaaaaagc gccctaaagg
    30181 cgcttttttg ctatctgcaa tttgcgaaat tgcctgatgc gcttcgctta tcagaccaac
    30241 tatgtccggc agttcccgtg cctctcatct actgcgccaa cgccgccagc agtttaacca
    30301 gcgcgggcgt cacctgctgc gtttcataaa caatatataa gtctgccggg atgcgttctt
    30361 tgagcggacg gaatatgaca ccgggccagt tcatctgcgc gtagctgtcc gctatcaatg
    30421 tgataccaat acccatactg accatggcga gtaccgtttg cggttcatta acttcgcgaa
    30481 taacaaccgg tgaaaatccc acctgctggc aaactcgctg caaaaaatcc cagtcagtgt
    30541 acacgggcgg cattgtaacg aaatactcgt cacgtagcgc ttccagcggg acggtggaaa
    30601 atgatgagag atgatgctct tcaggcatcg ccaccagaaa cgccgattca tgcaaccgta
    30661 agctggtaaa accagtcggt ggttctgtcg ccattcgcca gatcccggca tcaagttcgc
    30721 ggcgttccag caaggccatt tgcatcgcag gcatcttttc gcgaaacaga acttcaacgt
    30781 taggattttc tctaaggaat cgccgcataa ccgggcgcat ccgtccccac attgccgttc
    30841 ccacaacccc gagttcaatc cgccctgctt ctccccgacc tatttgttca acccgagcca
    30901 atacattatt tgcattcacc agcaatcgac gcgattcttc catcaagatt ttgcccgcgt
    30961 gtgtcagtac gacgctgcgc gaatggcgaa taaaaagctg cgtgccgagt tgattttcca
    31021 gctctttaat atgaatgctg agcggaggct gagacatatt taaacgcgct gctgcgcgac
    31081 caaaatgcaa ctcttccgct acggcaagaa aataacggag caacttaaga tctgttctgt
    31141 atacgcgttc cataattaag tgctccatat aataaataca tcgtattaaa ttatcatggc
    31201 ataaagtatt tcaccaccag atatccgaca tacataaaca caatggataa tatacccggt
    31261 aatacatttg gtacaggaat aggcaagcgc acgattgtaa acaaagcacc aatgccaaaa
    31321 ccaacgccag aagaaaataa aatatctttc actaattaac ctttatcata aaagcagccc
    31381 caaggaacag agctgcgaat ccttttaatg tgtaactgaa cgatgcgtta tcttttcagg
    31441 gtcatggtta tatttaaacc caaagaaaaa tatcactgcg aggaaaagag cataccctgc
    31501 aaacatcagc caaatggtat gccaatcttt tacgccatcc accgagaagt aatccactgc
    31561 cataccgctc agaatcgagc caacccatgc gccgacacca tttaccatgg tcataaaaag
    31621 gccctgggcg ctggcacgaa tgctggaatc aacttcctgt tcgacaaata ccgaaccaga
    31681 aatattgaag aaatcgaatg cacagccata aacaatcatc gacagcagca gcaaaataaa
    31741 cccggttggt gatgggtcgc cataggcaaa gaagccaaag cgcagcgtcc acgccaccat
    31801 actcatcagc atgacggttt taataccgaa acgcttcaaa aagaacggta tcgtcagaat
    31861 gaaaccgact tcagccatct gtgaaactga cagtaaaatg gacggatatt tcaccacgaa
    31921 gctatcagca aactcaggat tacgggcgaa atcatgcagg aacgggttgc caaaaacgtt
    31981 ggtaatttgc agcaccgcgc ccagcatcat ggcgaagagg aaaaagatag ccatgcgtgg
    32041 atttttaaac agcacgaagg catccagacc cagcttgctg gcaagcgatg tagtcgcttt
    32101 tttctccgca accggaatct tcggcaaagt cagcgcataa gccgacagca gcaatgacgc
    32161 accggacgcg atatacagct gcagactact caattccaga tgcagcaggc ttactgccca
    32221 catggcgaca atgaacccca ccgtaccaaa aacgcgaatg ggcgggaaag cggtcaccgg
    32281 gtcaagccct gcctgggcaa gacaggaata agagacgctg ttcgataacg caatagtcgg
    32341 cataaacgcc atcgcattga ctaacatcac ccaaaacatc atatccggat cagttacgga
    32401 tgccgcataa aaaagtacgc ccgcacacac caggtgacac agcatgtatg cacgttctgc
    32461 acgcagccat ttgtctgcga tgatccccat tatacccggc ataataatcg cggcgatccc
    32521 tttggaactg taaaccatgc caacattagc gccggtgaaa tgaagggtat taatcatgta
    32581 agaaccgagg gtaaccagcc agctccccca gataaaatat tgcaaaaagg acattacctt
    32641 taagcgcaaa gcgatgctca taatcgtttc cttgtcgcat catatggcct cactcagtaa
    32701 ggccgtttat tgttgtttta ctcaggcaat tttgcgcaga aagccgcaaa taagattaat
    32761 aaagttttgc tttgagagtt ccgctgctgc cagcgtttgg gcatgagaaa gcttcacgtc
    32821 actcagacct tccgccatgt tggtaatcgc agagaccgca acgactttaa gttcgcaatg
    32881 gcgagctgaa ataacctcag gcaccacaga cataccaaca acatccccac caataatttg
    32941 catcatacga atttccgccg cagtctcgaa attcggcccc ggatacgaga cgaacacgcc
    33001 ctccgtcaga gggaacccct cttctttcgc cactttttgt aacagtgcgc ggtattccgc
    33061 atcgtaggca ttcgccagcg agaagaagcg ctcaccaaaa cgttcatcat taagacccac
    33121 catcggcgtt cccggcatgg tgttgatgtg atctttcaat gcgaccagac tgccggcccc
    33181 cacttcagga cgcagtgagc cagccgcatt ggtgcagaac agcaactcgc agcccagcaa
    33241 cttaaaggta cggattgcat ccgtcatgat ggtcatccca cgaccttcgt agaaatgtcc
    33301 gcgacctttc atgcacgcca ctggcacccc ctggagatac cccagcacca gctcacccgc
    33361 atgaccgtgt acggtactta ccgggaaccc aggcagcttt tcgtaggaaa ttgcgaccgc
    33421 gttctcaatc tgatcggcca gcgcgcccag cccggaacct aaaataaagg ccactcgtgg
    33481 cgtgaaatca ggtttataag tcttgataat atctacgcaa tacagtgggt tatgagaaaa
    33541 ctgagcctga gacatatata tccttttttt gtagggtgga atctaacgcc tttttataac
    33601 gagattgccc tacctcatac caatacactt ttttcatgta tccgatagtt attcgatatc
    33661 gatatattta tattttttca ctattaatag gctgaaaaaa ctgtattggt actctccttt
    33721 cccttgcatt aacaatatgc cgcttttaat caacctttta ttaagcaatc aactaataac
    33781 gatcggcata gggaattatg ctatgaaaaa acatcttttt actctgacac tctcttctgt
    33841 attggcgata ccagcagtat cccatgcaga atttaaaggc ggttttgctg atataggtat
    33901 tcattatctt gactggacta gcagaaccac tgagaaatcg tcgaccaaat cacacaaaga
    33961 tgattttgga tatctggaac tggaaggcgg cgctaacttt agttggggag aaatgtacgg
    34021 gttcttcgac tgggaaaact tttataatga tcgtcatgat aaaccgggta gcgagcagcg
    34081 ttataccttt aaaaatacca accgtattta cctgggtgac accggattta atctctactt
    34141 gcatgcgtat ggcacctacg gttctgcgaa tcgcgtgaat ttccatgacg atatgttcct
    34201 ttacggtatc ggctacaatt tcaccggtag cggttggtgg ttcaaaccgt tctttgcaaa
    34261 acgctataca gaccaaacct attataccgg cgataacggt tatgtcgccg gttgggttgc
    34321 aggttacagc tttatgctgg gcagtgagaa attcaccctc accaactgga acgagtacga
    34381 gtttgatcgt gatgccacct acgcggcagg taatggcggc aaagaaggtc tgaacggtgc
    34441 cgtcgcactt tggtggaatg caacttcaca catcaccaca ggaattcagt atcgttatgc
    34501 ggatgacaaa ctgggcgaag atttttacca ggatgcaata atctactcca tcaaatttaa
    34561 tttctgattg attactgccg gggctcccgg cagtatgaat agctctatat ctgatacagc
    34621 ggatcccctt cccgcccagc cattagcgca cggccctggc ctcgtaccac ttcccataac
    34681 gcttgtgcgg cggtggaaag tgaacggttc ttcctgcgca ccagcatcaa ctggcgttcc
    34741 accaccggcg taatgcgctt taccaccagc gggctacctt caggtaacgg cagcgccagt
    34801 gcgggaagaa tactaatgcc gatgcctgcg gctaccatcg ggaacagcgt cgccggatga
    34861 ccaatctcct gcacaatatt cgcctgaata ccgttgcgcg ccagtgctgc atcaatcagc
    34921 ggtcggctgc ctgacgcgta atcctgcaac accaatttcg ctccctgtaa tgcctgccag
    34981 ggtacataat cttccgcggc caaagcgcta tcgcgatggc aaagcaagaa gaaaggctcg
    35041 gaaagaatcg cttcacattg cagatcgctc actgggcctg gatcgatgac gatgccaaaa
    35101 tcgacatctc cctggcgaat actttccatc acccactgct gcggacgatc gtgcaggata
    35161 aactgaatat ctggatagcg gcgatggctt tcagcaatgc attgcggaat aagatgcgcg
    35221 gaaatggtct ggctggcagc gacccgaact tttccgctca gttgttgccc catacgcccg
    35281 gtatcgcgca acgtgctgtt aagttcatcc agcagtcgct caagacgcaa agccaactgc
    35341 tgccctgcat ctgtaagcac cacttcacgc gtggttctgt ccagcaggcg aacaccagta
    35401 tgattttcca gctccttcac actgtgactc actgccgact ggctcaggcc gatacgctct
    35461 cctgcacgac taaaactttt ctcctgcgct actgtgacga aaacctttaa ttgttttaaa
    35521 gaataattca tcttttttct tcatgaatgg atgcaataaa tcaattttat ttctcaaact
    35581 ggaagaagca caatagaacc atcgatcatc tggagtcttt atgaaacttt ttcgtatcct
    35641 cgatcctttc accttaaccc tgatcacggt ggtgttgctg gcctctttct ttccggccag
    35701 aggcgatttc gtccccttct ttgaaaatct gaccactgca gctattgccc tgctgttctt
    35761 tatgcacggc gcgaagttgt cgcgtgaggc gattattgct ggcggtgggc actggcgact
    35821 gcatttgtgg gtaatgtgca gcaccttcgt attgtttccg attctgggcg tgctgtttgc
    35881 ctggtggaaa ccggtaaatg tcgacccgat gctctactcc ggttttctct acttgtgcat
    35941 tctccccgct accgtgcagt ctgcaatcgc cttcacgtca atggcgggcg gtaacgttgc
    36001 ggcggcggtt tgttctgcgt cagcatccag cctgcttggg attttccttt caccattgct
    36061 ggttggtctg gtgatgaatg ttcacggtgc gggaggcagc cttgagcagg tcggtaaaat
    36121 tatgctgcaa ttgctactgc cgtttgtgtt ggggcatctt tcccggccgt ggattggtga
    36181 ctgggtgtcg cgcaataaaa aatggattgc gaaaactgac cagacgtcca ttctgttggt
    36241 ggtttatacg gcattcagcg aagccgtcgt taacggtatc tggcacaaag ttggctgggg
    36301 atcattgctg tttatcgtgg tggtcagttg cgttcttctg gctatcgtga ttgtagttaa
    36361 cgtctttatg gcacgccgac tgggcttcaa taaggcagat gaaattacta tcgtcttttg
    36421 tggttcgaaa aagagtctgg caaatggcat cccgatggca aacattctgt tccccacatc
    36481 ggtgatcggt atgatggtgc tgcccctgat gattttccat cagatccaat tgatggtctg
    36541 tgcggtgctg gcgcgtcgat acaaacgcca gaccgaacag ttacaggcgc agcaggaaag
    36601 cagcgccgat aaagcttaaa gcggacgctt caggggctgg accagttgcg tcagcccctc
    36661 ggttttaatc agcagcgtga tttgcattag ctcaccgagc ttccctgccg ggaactcatc
    36721 tttgcgggca aaccacagca gatactcctc cggcaggtcg attaagcgac gccctttgta
    36781 cttgccaaac ggcattatcg tattggctat ttcaatcagc tgctcttttt ccatctcagc
    36841 tacccagcaa acgcagcatt tccgtttcgt cgatgacttc aatacccagt tcctgcgcct
    36901 tcgccagttt agatcctgca gcttcacccg ctatcaccag atcggttttc ttcgacacgc
    36961 tgcccgcgac tttcgcgccc agttcgacca gtcgagcttt agcgtcatca cgcgacatct
    37021 ggcttaagct gcccgtaagc accacggttt taccagcaaa cgggctgtca atctcttccg
    37081 cgttaatcac caccggcgca ggccagtgaa cgccttccgc caacagctcg ctgatgacat
    37141 tgcggttgct ttcttcggca aagaagttgt gaacgtggga ggcaacgaca atgccaacat
    37201 caggcacctt ttgcagctct tcaatcgaag cggcttccag cgcttccagc gtgccgaaat
    37261 atgccgccag acctgctgcg gtggcctcgc cgacttcacg gatgccaagt gcatagagga
    37321 agcgagcaaa ggtggtttct ttcgcttttt ccagcgcgtt aaccacgttt tgtgccgatt
    37381 tcggccccat acgctccagt ccggtcagtt tgactgcggt gagtttgaac agatccgccg
    37441 gagtgtggac atattctttt tcaaccagtt gatcgatgat tttgtcgccc attccgtcaa
    37501 catccatcgc acggcgggaa acaaagtgtt tcagagactc tttacgctgc gcaccgcaaa
    37561 tcaggccacc ggtacagcgg gcaaccgctt caccttccac acgctcgacg tcagaaccac
    37621 ataccggaca atgcgtcggg aatacaacct cacgggtatc ttccgggcgt tcagaaagca
    37681 cgacgttaac cacctgcggg atcacgtcgc cagcgcggcg aataaccact ttatcgccaa
    37741 tgcgtaaacc aagacgttcg atttcgtcag cattatgcag tgtagcgtta ctcaccaaca
    37801 cgccagcaac atggacaggt tccagacgcg caacaggcgt aatcgcgcca gtacgcccaa
    37861 cctgaaactc gacgtcacgc acaaaggtca tctgttcctg cgccgggaat ttaaacgcta
    37921 ccgcccagcg cggggcacgc gcgacaaagc caagctgctc ctgctgtgcc agtgagttga
    37981 ccttaatcac cacgccgtcg atatcaaagc ccagcgtcgg acggtctttt tctacttcgt
    38041 gataaaacgc cagcacttct tccgccgatt cacaaagtgt tacccgatca ctgacaggta
    38101 atccccacgc tttaaattgc attaagcgtc ccaaatgggt atccggcaat tcaccgcctt
    38161 ccagaacacc aacgccatag cagaagaaag tgagcggccg cttcgctgta atacgcggat
    38221 caagctgacg cagtgaacca gctgccgcat tacgtgggtt agcaaacact ttcccgcccg
    38281 tgcgtcgcgc atcttcgtta atcttttcga accccgcctg cggcaggaac acttcaccac
    38341 gcacttccag acgcgccggg atattctctc cgtgtagctt cagcggaatg gcgcgaatag
    38401 tacgcacatt agacgtgata tcttccccgg tggtgccatc gccacgggtg gcggcactga
    38461 ctaaaacacc attttcatac agaatactga cggcaagacc atccagcttc agctcacagc
    38521 accaggtgac tttctcgttg cttttcagac ggtcctgcac acgtttgttg aaagcaagaa
    38581 agctttcttc atcaaaaacg ttatccagcg acagcattgg cacttcatgg cggatctggc
    38641 tgaaagccgc cagcggcgca gcacctacac gttgggtagg cgaatcaggc gtaatcagtt
    38701 ctggatgttt ggtttccagc tcgcgcaatt cgcgcatcag cctgtcgtat tcagcgtcgg
    38761 gaatttccgg cgcatccatc acatgataaa gatattcatg atggcgaagc gtcgttcgca
    38821 gttctgtcag ttgttgttcg attgattcca tatcgcacca tcaatgctaa aaacccccga
    38881 caagcggggg ttcgaagagg agttaatttg ccttaagtgt atcaggcgtt ggcgtctttg
    38941 acttcgcgga tgatgtcctg gtactcgcgc aatttctgcg gagtcatcat acggcgctga
    39001 tcgtcaagaa caacaccgcc cacttcatcg gcaatatgct gcgcagattg cagcatcagc
    39061 ttgaagttct gcagctcgtc accgtaagac ggtacctgca taaagatagt gacacccgga
    39121 gtagtgaaat ccttcatttc aggatcaaag gttcccggtt tcaccatatt cgccaggctg
    39181 aataacgccg ggccgctgcc atccgggcta agatgacgat ggtaaatatt catatcgcca
    39241 aaaatgaagc ccgcttgttg aatgctgtta agaagcagtt caccgtttag ctcgctaccg
    39301 tgatgcgccg cgacgttcat gataatcacc gcttctttgc gcttcggttt atccataact
    39361 ggagccggtt ccgctacagg ctcaggctgt ggtgccgcta cgggttctgc aggctggaaa
    39421 gcctgttgtg ccggttgcgg tgctgaatgc actggctgcg gcgctggcgc gacctgtggc
    39481 gaaactggct gctgcaacgg ctgttcaggc tgcggctgat aggcaggctg ctgcacgggt
    39541 tgctgcacca gctgcgctgg acgcggagca tgttgcggcg gtacctgcgc ttcaggcggc
    39601 tgctggaccg gttgacgcgg ctgcgcagac gcataaggcg gttggtactg gtgttgcggc
    39661 gacggacgag cagcctcatg ctcctgggcg ttagccgggg catgattcac gcggtgaact
    39721 cgaacctcac caacgccctc atcatcttcg acatcctcgt cataagaatc gtcgtcacgt
    39781 tttgacttca ttcgttttaa tggccgatcg cggaacatag aagatcgttc tttacggctg
    39841 gtccagaaac catgtaccag taaagcgatt atggcgatcg cgccaacaat gattaatatc
    39901 agacgcaaat cctgcatcat tatattctct gttgttctaa caccttgcca ccacggcaaa
    39961 catttactca ctaagagtat ttgtcgatta cctcaagtgc aagtgcacta ttaactttca
    40021 cagcacaaag atagatgaaa tcgtgctttt tgctgttttt tcgaacatat cctaactgtc
    40081 cattgcgcaa ttacccggta aaatacgcag aattttcctg ggattggtca aaaggagctc
    40141 atcctgatta tggtttcatc attcacatct gccccacgca gcggttttta ctattttgcg
    40201 caaggctgga agctcgtctc gcaacctggg attcggcgtt tcgttatttt accgctgctg
    40261 gtcaatattt tgttgatggg gggcgcattc tggtggctct ttacgcaact cgatgtctgg
    40321 atcccgactc tcatgagtta tgttccggac tggttacaat ggctgagcta tctgttgtgg
    40381 cccctggctg tcatctctgt gctgttagta tttggttatt tcttttcaac gattgctaac
    40441 tggattgccg ctccgttcaa cggtttattg gctgaacaac tggaagcaag actgactggc
    40501 gctacaccgc cagataccgg aattttcggt atcatgaaag atgtgccgcg aatcatgaaa
    40561 cgcgaatggc aaaaatttgc ctggtatctg ccgcgcgcaa ttgtattgct aattctttac
    40621 ttcatccctg gtattgggca aaccgtcgcg ccagtactgt ggttcctgtt tagcgcctgg
    40681 atgttagcca tccagtattg cgattacccc ttcgataacc acaaagtgcc gtttaaagag
    40741 atgcgcaccg ccctgcgcac acgcaaaatc accaatatgc agtttggtgc tttaaccagc
    40801 ctgtttacga tgatcccgtt gcttaatctg ttcatcatgc ccgttgccgt ttgtggcgcg
    40861 acggcgatgt gggtcgattg ctatcgcgat aaacacgcga tgtggcggta acaatctacc
    40921 ggttattttg taaaccgttt gtgtgaaaca ggggtggctt atgccgcccc ttattccatc
    40981 ttgcatgtca ttatttccct tctgcatata gatatgctaa atccttactt ccgcatattc
    41041 tctgagcggg tatgctacct gttgtatccc aatttcatac agttaaggac aggccatgag
    41101 taagattttt gaagataact cgctgactat cggtcacacg ccgctggttc gcctgaatcg
    41161 catcggtaac ggacgcattc tggcgaaggt ggaatctcgt aaccccagct tcagcgttaa
    41221 gtgccgtatc ggtgccaaca tgatttggga tgccgaaaag cgcggcgtgc tgaaaccagg
    41281 cgttgaactg gttgaaccaa ccagcggtaa taccgggatt gcactggcct atgtcgctgc
    41341 cgctcgcggt tacaaactca ccctgactat gccagaaacc atgagtattg aacgccgcaa
    41401 gctgctgaaa gcgttaggtg caaacctggt gctgaccgaa ggtgctaaag gcatgaaagg
    41461 cgcaatccaa aaagcagaag aaattgtcgc cagcaatcca gagaaatacc tgctgctgca
    41521 acaattcagc aatccggcaa accctgaaat tcacgaaaaa accaccggtc cggagatctg
    41581 ggaagatacc gacggtcagg ttgatgtatt tattgctggc gttgggactg gcggtacgct
    41641 gactggcgtc agccgctaca ttaaaggcac caaaggcaag accgatctta tctctgtcgc
    41701 cgttgagcca accgattctc cagttatcgc ccaggcgctg gcaggtgaag agattaaacc
    41761 tggtccgcat aaaattcagg gtattggcgc tggttttatc ccggctaacc ttgatctcaa
    41821 gctggtcgac aaagtcattg gcatcaccaa tgaagaagcc atttctaccg cacgtcgtct
    41881 gatggaagaa gaaggcattc ttgctggtat ctcttctgga gcagctgttg ctgcggcgtt
    41941 gaaactacaa gaagatgaaa gctttaccaa taagaatatt gtggttattc tgccgtcatc
    42001 gggtgagcgt tatttaagca ctgcattgtt tgccgatctt ttcactgaga aagaactgca
    42061 acagtaatgc cagcttgtaa aaaatgcgta aaaaagcacc tttttaggtg cttttttgtg
    42121 gcctgcttca aactttcgcc cctcctggca ttgattcagc ctgtcggaac tggtatttaa
    42181 ccagactaat tattttgatg cgcgaaatta atcgttacag gaaaagccaa agctgaatcg
    42241 attttatgat ttggttcaat tcttccttta gcggcataat gtttaatgac gtacgaaacg
    42301 tcagcggtca acacccgcca gcaatggact gtattacgct cttcgtgcgt cgcgtctgtt
    42361 aaaaactggc gctaacaata caggctaaag tcgaaccgcc aggctagact ttagttccac
    42421 aacactaaac ctataagttg gggaaataca atgttccagc aagaagttac cattaccgct
    42481 ccgaacggtc tgcacacccg ccctgctgcc cagtttgtaa aagaagctaa gggcttcact
    42541 tctgaaatta ctgtgacttc caacggcaaa agcgccagcg cgaaaagcct gtttaaactg
    42601 cagactctgg gcctgactca aggtaccgtt gtgactatct ccgcagaagg cgaagacgag
    42661 cagaaagcgg ttgaacatct ggttaaactg atggcggaac tcgagtaatt tcccgggttc
    42721 ttttaaaaat cagtcacaag taaggtaggg ttatgatttc aggcatttta gcatccccgg
    42781 gtatcgcttt cggtaaagct ctgcttctga aagaagacga aattgtcatt gaccggaaaa
    42841 aaatttctgc cgaccaggtt gatcaggaag ttgaacgttt tctgagcggt cgtgccaagg
    42901 catcagccca gctggaaacg atcaaaacga aagctggtga aacgttcggt gaagaaaaag
    42961 aagccatctt tgaagggcat attatgctgc tcgaagatga ggagctggag caggaaatca
    43021 tagccctgat taaagataag cacatgacag ctgacgcagc tgctcatgaa gttatcgaag
    43081 gtcaggcttc tgccctggaa gagctggatg atgaatacct gaaagaacgt gcggctgacg
    43141 tacgtgatat cggtaagcgc ctgctgcgca acatcctggg cctgaagatt atcgacctga
    43201 gcgccattca ggatgaagtc attctggttg ccactgatct gacgccgtcc gaaaccgcac
    43261 agctgaacct gaagaaggtg ctgggtttca tcaccgacgc gggtggccgt acttcccaca
    43321 cctctatcat ggcgcgttct ctggaactgc ctgctatcgt gggtaccggt agcgtcacct
    43381 ctcaggtgaa aaatgacgac tatctgattc tggatgccgt aaataatcag gtttacgtca
    43441 atccaaccaa cgaagttatt gataaaatgc gcgctgttca ggagcaagtg gcttctgaaa
    43501 aagcagagct tgctaaactg aaagatctgc cagctattac gctggacggt caccaggtag
    43561 aagtgtgcgc taacattggt acggttcgtg acgttgaagg tgcagagcgt aacggcgctg
    43621 aaggcgttgg tctgtatcgt actgagttcc tgttcatgga ccgcgacgcg ctgcccactg
    43681 aagaagaaca gtttgctgct tacaaagcag tggctgaagc gtgtggctcg caggcggtta
    43741 tcgttcgtac catggacatc ggcggcgaca aagagctgcc atacatgaac ttcccgaaag
    43801 aagagaaccc gttcctcggc tggcgcgcta tccgtatcgc gatggaccgt aaagagatcc
    43861 tgcgcgatca gctccgcgct atcctgcgtg cctcggcttt cggtaaattg cgcattatgt
    43921 tcccgatgat catctctgtt gaagaagtac gtgcactgcg caaagagatc gaaatctaca
    43981 aacaggaact gcgcgacgaa ggtaaagcgt ttgacgagtc aattgaaatc ggcgtaatgg
    44041 tggaaacacc ggctgccgca acaattgcac gtcatttagc caaagaagtt gatttcttta
    44101 gtatcggcac caatgattta acgcagtaca ctctggcagt tgaccgtggt aatgatatga
    44161 tttcacacct ttaccagcca atgtcaccgt ccgtgctgaa cttgatcaag caagttattg
    44221 atgcttctca tgctgaaggc aaatggactg gcatgtgtgg tgagcttgca ggcgatgaac
    44281 gtgctacact tctgttgctg gggatgggtc tggacgaatt ctctatgagc gccatttcta
    44341 tcccgcgcat taagaagatt atccgtaaca cgaacttcga agatgcgaag gtgttagcag
    44401 agcaggctct tgctcaaccg acaacggacg agttaatgac gctggttaac aagttcattg
    44461 aagaaaaaac aatctgctaa tccacgagat gcggcccaat ttactgctta ggagaagatc
    44521 atgggtttgt tcgataaact gaaatctctg gtttccgacg acaagaagga taccggaact
    44581 attgagatca ttgctccgct ctctggcgag atcgtcaata tcgaagacgt gccggatgtc
    44641 gtttttgcgg aaaaaatcgt tggtgatggc attgctatca aaccaactgg aaacaaaatg
    44701 gtcgcgcctg tagacggcac cattggtaaa atctttgaaa ccaaccacgc gttctctatc
    44761 gaatctgata gcggcgttga actgttcgtc cacttcggta tcgacaccgt tgaactgaaa
    44821 ggcgaaggct tcaagcgtat tgctgaagaa ggtcagcgcg tgaaagttgg cgatactgtc
    44881 attgaatttg atctgccgct gctggaagag aaagccaagt ctaccctgac tccggttgtt
    44941 atctccaaca tggacgaaat caaagaactg atcaaactgt ccggtagcgt gactgtgggt
    45001 gaaaccccgg ttatccgcat caagaagtaa ttcttgccgc agtgaaaaat ggcgcccatc
    45061 ggcgccattt tttatgcttc cgccagcggc ggcaaaatca attcatcgct ctcatgctgc
    45121 tgggtgtagc gcatcacttc cagtacgcgc aaccccgcac ggtgcaccgc atcgtttaac
    45181 gccttccctt tcagcaatcc actgataagc tgagcacaaa acaggtcgcc agtccctttc
    45241 aggtcggttt ttacccgtga atgggaaatg acattcacgc tgtcggcact gaccaccacc
    45301 acctgcatct cctgattttc ttcattaccg gaggcgctgg taatcaccac ccattttaat
    45361 gtgtctgaaa gcagactttt tgcggcagca atggcactgt cgagatcgcg gcaattttta
    45421 ccggtcagga tttccagctc aaagatattg ggggtaattc cctgcgccag cggcagtaaa
    45481 tattgtcgat acgcctcggg aaggtcaggt ttgacataaa ttccgctatc aatatcgcca
    45541 atcaccggat cgaccatgat caataggtca ggatggtctt tgcgtagcgc agtcagccac
    45601 tcggcaagga ttttgatttg cgatgccgtt cccatatagc ccgtggttac agctcgaagt
    45661 tggcgcagcg catcacgctc ctgaagcgca cgcaaatagc cgctaaacca ttcatccgga
    45721 atcgcaccac cgtagaaagt gtcataatgc ggtgtattgc tcagcaatac cgtcggcacg
    45781 gcaaagacat tcaggccgtt ctgtttgata gcaggcaccg caatgctgtt gcctacgctg
    45841 ccgtacacta cctgcgactg cacggcgacg atatccgcct gcaatgccct gctcttatcg
    45901 ttaaacaaca acaaactact catttaattt tttctccttg ccgatgatcc tcatcgtatt
    45961 ccaaccgaaa ctttacctga ttctggcagt caaatcggct atcacaaaac aaggataagg
    46021 taattcaatg aagaaaatca tttgtctggt cattacgctg ctaatgacac tccccgttta
    46081 cgcgaagtta actgcccatg aagaagcccg tatcaacgcc atgctggagg gattagcaca
    46141 gaaaaaggat ttgatatttg tgcgtaacgg cgatgatcat acctgcgatg aagcggtttc
    46201 ccacctgcgt ctgaagctcg gcaatacccg taaccgcatt gacactgccg agcagtttat
    46261 tgataaggta gcctcgtcgt catcgattac cgggaagccg tatatcgtga agatgcccgg
    46321 taaaagcgat gagaacgcac aacctttttt acatgcgctg attgcgcaga cggataaaac
    46381 ggtgcctgcg caataatctt aaatccccgc cccctggcta aaatgctctt ccccaaacac
    46441 cccggtagaa aggtagcgat cgccgcgatc gcagatgatc gccacaacca ccgcgccggg
    46501 gttagctttt gccacgcgca gtgctccggc aaccgcgccg ccggagctga cgccacagaa
    46561 tattccttcc cgcactgcca gttcgcgcat ggtgttttcc gcatcgcgct gatgaatatc
    46621 cagcacctca tccaccagcg aagcgttgaa aatccccggc agatattccg caggccagcg
    46681 gcgaatgccg ggaatgctac tgccctcttc cggctgcagg ccaacaatgg tcaccggttt
    46741 ggattgttcg cgcataaagc gtgaaacgcc ggtgatagtg ccggttgttc ccatgctgga
    46801 gacaaaatgg gtaatgcgcc cgccggtttg ctgccagatt tccggcccgg tggtggtgta
    46861 atgggcgaag ggattatcag gattattgaa ctgatcgagc agctttcctt caccacgatt
    46921 ggccatctcc agcgccagat cgcgcgcgcc ttccatgccc tgctctttgg tgacaagaat
    46981 cagttccgca ccgtatgcca gcatcgctgc acggcgctcc tggctcatgt tgtcgggcat
    47041 cagcaatttc atgcgatagc ctttaagcgc ggcaatcatt gccagcgcaa tgccggtgtt
    47101 accactggtg gcttcaatca ggacatcgcc cggtttgatt tctccgcgct tttccgcctc
    47161 gacgatcatc gaaagcgctg cacgatcttt caccgaacct gccgggttat tgccttccag
    47221 tttcaaccag acttcactgc cgttatccgg cgccattcgc tgcaacttca ccagaggcgt
    47281 attgccaatt gtttgttcta atgtactcac gatctctgtc catacgtggt gttgcctgat
    47341 gcgacgcttg cgcgtcttat caggcctaca ggttacaaac cttgccatta aaaagcccgg
    47401 atcgcgggaa gcgcctccgg gcgtttaaca ttcactcaac ctatcaggcg ctttgtgcga
    47461 gagcaagttc ctcatcgcgg gtttcaatac gctcgtcgcc gttatacagc cgcgcatgtt
    47521 gcagaccaac gaataaacgc tcgccacgct gcggggcatc gtcgccatgc atcacaaccg
    47581 tcagcggttc gttgtaccac cccagcggct gcacgactaa ttgggtgtag tggcctttcg
    47641 ggctggcttc cagtacctgt accggcagcg gcgaatcgag gctggtacgg cggctgatat
    47701 ccacttccca cggacgcagg aagagatcca ccggcccctg atacgcaggt gtatagccca
    47761 gcggccagcg atgtgcgcca acatggaact gcccgccgcg aatggttccc tgcaggcggt
    47821 tcacttcgcc cataaattcg agtacaaaac gggtcgctgg ttcgcgccat acctggtccg
    47881 gtgcatcagc ctgctcgata ttgccctggc tcatcaccac tacacgatcg gctacttcgg
    47941 tcgcttcttc ctggtcatgg gtcacaaaca cgctggtgaa tttcagctct tcatgtaact
    48001 gacgcagcca gcgacgcaac tctttacgca cctgcgcatc cagcgcgcca aacggttcat
    48061 caagcagcag aatttgcggt tctacagcca gcgcgcgcgc cagtgccaca cgctgtttct
    48121 gaccgccgga aagctgtgct ggataacgat ccgccagatg cgcaagctgc accatctcca
    48181 gcaattttgt cactttcgct ttgatggctg cggcattcgg gcgctcgcga cgcggcagca
    48241 ccgtcaggcc gaaagcgata ttgtcgaaca ccgtcatatg gcggaacagc gcatagtgct
    48301 ggaacacgaa accgacttta cgatcgcgcg catgcaggcg gctgacgtcg gtgccgtgga
    48361 agcgaatatg cccgctggtt tgatgctcca gcccggcgat aatgcgcagc agcgtggttt
    48421 tcccggaacc ggacggcccc agcaacgcga ccatctgacc agaaggaata tccagtgaga
    48481 tatcgttcag cacctgggtg cgaccaaaag acttcttaat attggcaatc tcaatgctca
    48541 tgatgttcct cctgctgcgc gcgtttttcc tgattctcca gacgccactg caacatactc
    48601 tttaaaaaca gggtgataat cgccatcagc gtcaacaacg cggcagcggt aaaggagccg
    48661 acggtgttgt agtcttgctc cagcaattca atctgtaacg gcagcgaaag tgtttcgccg
    48721 cgaatcgagc cggaaaccac cgacaccgca ccaaactcgc caatcgcgcg ggcgttggtc
    48781 agcaccacgc cataaagcag cgcccagcgg atgttcggca gcgtgacgcg gcgaaacatc
    48841 tgccagccag acgcgccaag caaaatcgcc gcttcgtctt cctggctgcc ctggcttaac
    48901 atcaccggca ccagctcacg caccacaaac ggacacgtca cgaagatggt gaccagcacc
    48961 attcccggcc aggagaacat aatttgcaga ttatgttcat ccagccagcc gcccagcggg
    49021 ccgttagagc cgtagaacag cagatacacc agaccagcaa ccaccggcga cacggcaaac
    49081 ggaatgtcca acagcgtcag caatagctgg cgtccgggga agttaaagcg cgtcaccagc
    49141 caggccagca gaataccgaa caccaggttt accggtacgg caatcagcgc gatcatcacc
    49201 gtcagccaga tggcgtgcag catgtccgga tcggccagat tctgtaaaac cggcatcagc
    49261 cctttgctga acgcctgtac gaagatataa atcatcggca ccagcaggat gaacgccgat
    49321 accagcatcc cgatgccaat cagaaaccat ttgccccagt taatcgggcg cgcgtcataa
    49381 cgcttcaact gtgtcacttc cgccattaat gacctaccac acgccgacca aagcgacttt
    49441 gcagagtgtt aattgagaac agcagcagca gcgatgccgc gaggatcacc gaggcaatcg
    49501 cgctcgccgc cgggtaatca aactcctgta agcgaacaaa gatcatcagc gacgtcactt
    49561 ctgttttcca ggcgatattc ccggcgataa aaatcaccgc gccaaactca ccgaggctgc
    49621 gggtaaacga cagcgccaca cccgccacca gcgccggaga tagttccggc agcaccactt
    49681 tgcgaaaact ctgccagcgt gttgcgccga gcgtttctgc cgcctcttca tattccgggc
    49741 ctaactcttc cagcaccggc tgcacggtgc gcaccacaaa cggaatgctg gtaaaggcca
    49801 tcgccaccgc aatccccagc caggtatagg tgactttgat atcaaacttc gccagccatt
    49861 caccgtaaaa accgttcacg gaaaagagcg aggccagcgt taaaccggcg actgccgttg
    49921 gcagcgcaaa gggtaaatcc atcagcgcat caagcagcgt gcgacccgga aagcgatagc
    49981 gggttaagat ccacgccatc agcagaccga aaacgccgtt aaaaatcgat gccacaaacg
    50041 ccgacagcag cgtcacttta taggctgcga ccacctgcgg gttggtgatc acctcccagt
    50101 actgcgccca gctcatctgg gccagttgca tcaccagcgc ggagagcggc agcagcaaaa
    50161 tcaggcacac aaacagcaga ctggtgccga ggcttaaggt aaagcccggc agcacgcgtc
    50221 ttgaagaaac tgcaaacatt acttacgccc cgccgctaac agcttgtcta actcgccgcc
    50281 gctggtgaag tgggttttca tcacttccgg ccaggagcca aatttatctt ccacgcggaa
    50341 cagctcggtc tgcgggaatt tatctttcag tttgcccatg acttccgggt tatttacgcg
    50401 gtagtaatag tcggtgatga tggtttgcgc ctgcgggctg tagagccagt tcaggtaggc
    50461 ttttgccgct ttttccgtgc cgttggcctg tacgttttta tccacccacg ccaccgggaa
    50521 ttccgccaga atgttggttt tcggaatcac cacttcaaag ccctgcgctt catactgttt
    50581 acggatgttg ttcacttccg actcgaagct gatcagcaca tcgcccaggc cgcgctcggc
    50641 aaaagtggtg gtcgcgccac gaccgccagt atcgaacact tcaacgtttt tcaggaactg
    50701 ggtcataaac tgttcggttt tggctttatc accaccgtcg gctttgtccg ctgcgcccca
    50761 tgccgcaagg taggtataac gcgcgttacc cgacgttttc gggttcggga aaatcagctt
    50821 cacgtcagag cgtaccaggt cgttccagtc gtggatattc tttgggttgc ccttacgcac
    50881 caggaagccc atggtggagt agaacggcga gctattattc ggcaggcgcg actgccagtc
    50941 cgctgggatc agcttgcctt tatcgtgcag gatttgtacg tcggtcacct ggttataagt
    51001 gacaacgtcg gcttttaaac cctgcaaaat tgccagcgcc tgttttgatg atccggcatg
    51061 agattgttta atcgtcagtt tgtcgccacc gttatctttt gcccattgtt gctcaaacgg
    51121 cgggttcagg gcggcaaaca gctcgcggga gacgtcataa gaactgttca gcagttccgt
    51181 tgcctgtaca tggcccgcca gtagcagaga agcaaccagc acgagtgagt tctttttcag
    51241 taagttaacg gccattgcgc acccttataa atttaatgac tttctaatag ccatcatatt
    51301 tataacggac gtgaaaggag taacggtttt atataccgtt tggtgatttg gaagttgaaa
    51361 agggaataag acgaaatcct ccccacaaat gcagggagga aacagaggtg aatcagatgc
    51421 cgacgctaac cgtctccggc agtgtgctgc cgccatcaat cacattctgt gtaccggtta
    51481 aatagctgga ttcatccgat gcgaggaagg ccgccagttc gccgacttcc agcggatcgg
    51541 cgaggcgacg catcgggatt gctttcgcca tttcagtcag caccgattct ggatcttccg
    51601 ggttcgactg gcgggcaatg ctttccgcca ttggcgtgcg aacgtatcca gggcagatgg
    51661 cgttaacgcg aataccagac tgcgcgtact ccaccgccag cgatttagtc aggccaacaa
    51721 tcgccgcttt cgttaaggcg tacgccgttt cgcccgggtc tgccaccata tcgccagtga
    51781 ctgaagacat catcacaatc cggccatctt tgcgggcaat catctccggc agcaccgcct
    51841 tcgtgacgtt ccatacgcct tttatattga tatcaatatg gaaatcgcga tcttcatcgc
    51901 tcatatcaag gaaactgccc agacgacaaa cgcctgcgtt attcaccaga atatcaatgc
    51961 gcccttcttt ttccttagcg cgcttaatag ctgcggcgac cgacgccggg tcacgcacat
    52021 cggcgacaac cgccgtacag cgatgaccac ggccacacag ttcgtccgcc agcttttcga
    52081 tctcagggga gatatccagc aagattaggt ttgcgccatg acgcgcaaaa gttctggcaa
    52141 ttccttcgcc aattccctgc aatgcgcccg taatcagtgc tgtcttgccc gtgagtttac
    52201 ccattttaat gctctccttg ttaatacaga gcgttacact ccattccctc tgaaaataca
    52261 ggataaacaa aatattgctg cggatcagct cactaattca ggaaaatctc aatccagtcg
    52321 ctgctttatg catcatttca ggcgttgcgt tagctcactg ctgcgctcaa tcattttcaa
    52381 tgactccaca tcattgagtt gcacaaggcc aacaaacagt aaatcggtaa cagaattttg
    52441 cgccgtgcgg gtggacatcg acgagctgcg ccattcggtt tcgccagaaa cggtatcgag
    52501 ggtgtaatgc gccagccgcc gcagcgggga atcggttaat gaggtaatgg caatcaccgt
    52561 tgccccctgc tttcgcgctg cctcggcgca caagacaatc tcttttttac tgccactgta
    52621 agagatagcg atttgtacat cgcctttttt tagcgcctga gaaacggtgg cttgtacgtg
    52681 ggtgtcggcc tcacaggcca cgcgataacc aattttcatc agtttgaacg acagatcgcg
    52741 ccccaccagc tccgacccgc ccaggcccgt tatctggata aacggtgctt tactgatgac
    52801 ctcaatgatt ttttgcagcc gcgcgtagtc aaacaacgcg caggtttgct ccagcgcctg
    52861 ctctttttcg cgattcagtt tacgggcaat cacttccagc gaatcatcac tggtaatcga
    52921 actgtgcagg tgcagcgccg tggcattcgt tttctcccgg ctggcactgt attcgccaat
    52981 taacgccata cgcaattcag taaatccttg cgcgccgagc ttctgggcaa acttcacaat
    53041 actcgactgg ctgatgccga gctgtttcgc catcttgcgc gaagagacgg atttcagctc
    53101 actcacgttg gcccgcagaa agtcagcaat tttttgctcg ttcccggtaa attcactttc
    53161 cgcattgcgt attttcgtca ggtacaacat cttccactct cctgggctgg cagcaaaaat
    53221 tccgcttaag ttcttatgac gctcttcaca ctctgcgagt gtagactcaa tgattccttt
    53281 aagtctgtca attcgggata tatgattcca tacatctgca attatttgat cgtaaatagt
    53341 aaggtcacca ccgatgcaac ttgaaaagat gattaccgaa ggctcgaacg ccgcttcggc
    53401 tgaaattgac cgcgtatcaa cgctggaaat gtgccggatt atcaacgatg aagataaaac
    53461 cgtaccactc gccgttgagc gcgtactgcc ggatatcgcc gcagcgatcg atgttatcca
    53521 cgcccaggtc agcggcggcg gtcgtctgat ttatctcggt gcgggaacat caggtcgtct
    53581 ggggattctg gatgccagcg aatgtccgcc gacctacggc gtgaaaccgg gtctggtggt
    53641 tggattaatt gctggcggcg aatatgccat tcagcacgcg gtggaaggcg cggaagatag
    53701 ccaggaaggc ggtgtgaatg atctgaaaaa catcggatta accgcgcagg atgtggtggt
    53761 tggtattgcc gccagtggtc gcacgccgta tgtgattgcc ggactggaat acgcgcgcca
    53821 gctcggctgc cgcacagtgg gaatttcctg taatccaggg agcgcggttt ccaccaccgc
    53881 tgagtttgct attacgccga tcgttggtcc cgaggtggtt accggttctt cacgaatgaa
    53941 agcaggcaca gcgcagaaac tggtgctcaa tatgctctcc accgggctga tgattaaatc
    54001 cggcaaagtg ttcggcaacc taatggtcga tgtggtcgcc accaacgaaa aattgcatgt
    54061 gcgccaggtc aatatcgtca aaaacgctac cggatgtagc gcagagcaag cggaagcagc
    54121 gttgattgcc tgcgagcgca actgtaaaac ggccattgtg atggtgctga aaaatctcga
    54181 tgccgcagaa gctaaaaaac gcctggatca acacggcggc tttattcgtc aggttttaga
    54241 caaggaataa cccatggcca aagagatcag cagtgaactt ctgaacacca ttcttacccg
    54301 ggtcggtgga ccgggaaata tcgccagttg tggtaactgt atgacgcgcc tgcgtctggg
    54361 tgtacatgac agttcactgg ttgatcccaa tatcaaaacg ctggaaggcg tgaagggcgt
    54421 cattttaacc agcgaccagg tgcaggtcgt ttttgggcct ggtaaagcgc atcgtgccgc
    54481 aaaagcgatg agcgagctgc tgggagaagc accggtacag gatgccgcag aaatcgccgc
    54541 ccagaacaaa cgtcagttaa aagccaaaca aacctccggc gtgcaacaat ttctcgccaa
    54601 attcgccacc atcttcacgc cgctgatccc cggttttatt gccgctggtc tgctgctggg
    54661 gatagcgacg ttaattgcca cggtgatgca cgttccggca gatgctcagg gcacgctacc
    54721 cgatgcgctg aattttatga aggtgttcag caaaggcctg ttcaccttcc tggtgattct
    54781 ggtgggctat aacgccgccc aggcatttgg cggcacgggc gtcaatggcg caattatcgc
    54841 cgcgctcttt ttgctcggtt ataacccaac tgcaaccacc ggttactacg ccggatttca
    54901 cgatttcttt ggtctgccca tcgatccgcg cggcaatatt atcggcgtgt tgattgccgc
    54961 gtgggcctgc gcccgcatcg aaggcatggt gcgccgcttt atgccggacg atctcgacat
    55021 gctgttgacc tcgttaatca ccctgctaat caccgctacg cttgcgtacc tgatcattat
    55081 gccgctgggc ggctggctgt ttgaaggcat gtcgtggctg tttatgcacc tgaacagtaa
    55141 tccgctcggt tgtgcagtat tagccgggct gttcctgatc gccgtggtgt ttggcgtgca
    55201 tcagggcttt attcccgttt acctcgcgtt aatggacagt cagggattca acagcttatt
    55261 tcccatcctt tcaatggcag gcgcgggcca ggtgggcgcg gcgctggcac tctactggcg
    55321 ggcgcaaccg cacagtgcgc tacgcagcca ggtgcgcggg gcgattattc ccggtctgtt
    55381 gggcgttggc gaaccgctga tttacggcgt caccctgccc cgcatgaaac cgtttgttac
    55441 cgcctgttta ggtggcgcgg cgggtggttt gtttatcggg ctgatagcct ggtggggcct
    55501 gccgatgggc ttaaacagtg cctttgggcc gtctggtctg gtggcgctgc cgctgatgac
    55561 atcagcacaa ggtatccttc cggcaatggc ggtttatgca ggcgggattc tggtggcatg
    55621 ggtttgcggg tttattttca ccacgctctt tggctgccgt aacgtcaatc tggactgata
    55681 atatgaaacg gacaatgctc tatctttctc tgctggctgt cagctgtagc gtcagcgccg
    55741 caaaataccc tgttctgaca gaaagttcgc cagagaaagc agggtttaac gtcgaacggc
    55801 ttaatcagat ggatcgctgg ataagccagc aagtggacgc cggttatccc ggcgtaaacc
    55861 tgctgattat aaaagataat cagattgttt atcgcaaagc ctggggcgcg gcgaaaaagt
    55921 acgatggcag cgcgttaatg gcgcagcctg tcaaagccac caccgggacg ctgtatgacc
    55981 tggcttccaa taccaagatg tacgccacta acttcgccct gcaaaagctg atgagcgaag
    56041 gcaaactgca tcctgacgat cggattgcga aatatattcc agggtttgcc gacagcccga
    56101 acgatgcgat caaaggcaaa aacacgctgc ggatttctga tctgctgcat cacagcggcg
    56161 gtttcccggc agatccgcaa tacccgaata aagcggtcgc gggcgcgtta tattcccagg
    56221 ataaaggcca gacgctggag atgatcaagc gcacaccgct ggagtatcaa ccctgcagca
    56281 agcatatcta cagcgatgtc gattatatgc tgcttggatt tatcgtcgag tcggttaccg
    56341 gtcagccact tgaccgctat gttgaagagt cgatttatcg cccgctcggc ctgacgcaca
    56401 cggtatttaa tccgctgctg aaaggcttta aaccacagca aattgccgcc acggaactga
    56461 acggcaatac ccgcgatggc gtgattcact tcccgaatat ccgcacttcc actctctggg
    56521 gtcaggtgca cgatgaaaaa gccttttatt cgatgggcgg cgtttccggg cacgcaggtt
    56581 tgttttccga taccggcgat attgcggtgt taatgcaaac gatgctgaac ggcggtggct
    56641 atggtgatgt gcagctgttc aatgcggaaa cggtgaagat gttcaccacc agctctaagg
    56701 aagatgccac ttttggcctt ggctggcgcg tgaatggtaa tgccaccatg acgccgacgt
    56761 ttggcacgct ggcaagcccg caaacctacg gtcacactgg ctggaccggg acggtgaccg
    56821 ttatcgatcc ggtgaatcat atggcgattg tgatgttaag caacaagcca cattcgccag
    56881 tagccgatcc gcaaaagaat cccaatatgt ttgaaagcgg tcagttgccg attgcaactt
    56941 atggttgggt tgttgatcag gtgtatgcgg cgttaaagca gtaataaaaa ggccagtcag
    57001 ttgtgatact ggctggcctt taatcatcga atcagaaacg cttacaacgc cattaacttg
    57061 tccagcgacg gcgcgaagta atagccgcca gtgaccggtt tggtaaaacg caacatcgca
    57121 tcacgtttgc catcggtatc gccaaacatg ctcagcagtt gctgctcaat gttatgcaga
    57181 cgcgcgcagt aggcgcagaa gtacagaccg tgcgtaccgc tggcggtgcc gtacggcagg
    57241 ctctgtcgaa caatcttcag ccctttgcca tcttctttca gatcaacgcg ggtcaggtga
    57301 gaggtttccg gacgttcgtc gccgtcaatc tcttcgttgg cctctttagt gcgcccgatc
    57361 atcatctctt gatcgtgaat gctcatccgg ttgagttgct tcaggttgtg ctcccaacgc
    57421 tgcacaaaca catagctgcc gccggcatcc acgccatctt tgataaccgc cacttcgcgg
    57481 cgcgtctctt cacccgccgg gttttctgta ccgtcaacaa agccgctcag gtcacgctct
    57541 tcaacccaac ggaagccgtg gatctcttct ttcacttcaa tgcaatcacc aaacgcttcc
    57601 atcgccgcct gggcgacaga gaagtttacg tcgtgacgca gagaaagaat gtggatcaac
    57661 acatcgaact gggtcgtcgg tgcaagacct ttaccgtagc ccggaaaatc tttcagctct
    57721 tcggccccaa cgccgccgct cagagcgcgc caggtattgt taccaaaggc aaccaccgca
    57781 ccaagatgcg cgtccgggaa tttcgcttca aaagttgcca gcttgtcggc aaatgtttta
    57841 ctggccgcac gcagggcgtc aacttcccct ttcacgttgg cttcgatcca aatcgccgcg
    57901 cggcaatgtt ctggcaaaat gccactctga acctgagaca ttgttcctcc tgaaaataag
    57961 aatgccacgc aatccgtggc attgttgggc gctattttac gtgttttttc gcggcggcgg
    58021 tttgttcaga cgcaaattaa cgccgccaga taattttact gactttccag tttttcaggg
    58081 tatcgtccga aggcattaac ccttcaggac cactccattc cccgctgaac tggtagctaa
    58141 tatgttgact gccttcggct ttacattcga cggcacgatt atcatcacca tcagcttttt
    58201 ggcaattgcc aaatgctttg ctgtaaaggt cgctaaacgg tgtaccgatt ttaacaccgg
    58261 tgtcagcagg aatatcgcta tccagcacgt caatgcgact gatcgtgcct tgatcgccgt
    58321 taatcaccat cgccacgttg tcgcccttca tcacttcaaa aaagcgcacc acgttgccgt
    58381 tcgcggtttt cattccgctg cgcaggcgat aatcgccatc aagcgcatcg gcaatggctt
    58441 gttcttgcag tggtgtggac gccgttaatt caccaacgcc ctgctcgctc actttggtgg
    58501 atgatccaaa ccagttccac ggattagcgg cagaccagtt aacggacgac atcgtggaac
    58561 agccggtcag catcaacggc atagcgcata acattaaacg cagcgatttc atgtcacttc
    58621 ctttggttat tcaataacgt tgcttggagt gcaaattcac caaaaagtgc cgttattctt
    58681 tttcttcatt aaaacaagcg cgtaaacgtc gattggtcag cagccagatt aacgccacaa
    58741 tatccgccac taccagcgcc agaccgatac cgctaacgga ttcaccgttc agccacaacc
    58801 acggctgcca gcaaagtaaa accacttgcg ccaacagcaa cagaaaatag agcacatgcc
    58861 agatacgggg aaatgaagcc cgccgaccac tcagcaaaaa cgccagcacc gccggaatgc
    58921 cgggaatcaa ccccagccag aaattatcgt gatcgggata aaacagattt agcagtgcag
    58981 taccctgctc gcgcgacgca ccggcaatga caaacagcac ccaggttcgc gcctgaagca
    59041 atagcacaag ccagaagagc aagggtaaac gcaggcgacc gtacgcatca taatggacag
    59101 gatgaaactc agtactcttc atcttcaatc aaacgcttac ccagactcag cacgtcggcg
    59161 tgttcatatc ccaggcgttc atacattccg agcaccatgt cgttatcttc cggcacgttg
    59221 atctgaattt tcgggcagcc gcgagcaatc agctttttct ccaaccgatt aagcaacgca
    59281 ttagcaatcc cacgcccacg aaactctgga tgcacgccaa gataataagc agacccgcga
    59341 tgcccgtcat aaccgcccat caccgttccg accacttcac cgttcacctc agcgaccaga
    59401 aacaaactga cgtcatggtt catcttacgc tcaatgtcca tttccggatc gttccacgga
    59461 cgcagcaagt cgcaacgctc ccaaagggtg atgacctctt cgaaatcttc ctggcgaaat
    59521 acgcgtatct ccatggtatt cgttaccttt ttgcgggtta aaaggctgat tatggcgtga
    59581 acggtcgaat tagccaatat ctgacgaaaa tcggttgaaa aagtggcata atggggagtt
    59641 gtcaactatt gaaatgaaaa gtaaaacaat tctcaacagc aaacggtcgt aacggattac
    59701 gcgatacgat ataacatctg gaactttatt attacaactc aggccgtatg agcactttta
    59761 aaccactaaa aacactcact tcgcgccgcc aggtgctgaa agcaggactt gcagcgctga
    59821 cgttgtcagg aatgtcgcaa gccatcgcca aagaagaacc tttaaaaacc agcaacggac
    59881 acagcaagcc gaaagcaaaa aaatctggtg gcaaacgtgt cgttgttctc gatccaggcc
    59941 acggcgggat tgataccgga gcgatcggac gcaacggttc aaaagaaaaa catgtggtgc
    60001 tggcaattgc caaaaacgtc cgttccattt tgcgtaatca tggcattgat gcgcgtttaa
    60061 cgcgttctgg cgatacgttt atcccactgt acgatcgcgt tgaaatcgcc cataaacatg
    60121 gcgcagatct gtttatgtca attcatgccg acggctttac caacccgaaa gctgccggtg
    60181 cttcggtatt tgccctctct aaccgtgggg caagtagcgc aatggcgaaa tacctgtctg
    60241 aacgcgaaaa ccgcgccgat gaagttgccg gtaaaaaggc gactgacaag gatcacctat
    60301 tgcagcaagt gctgtttgat ctggtgcaaa ccgatactat taaaaacagc ctgacgctcg
    60361 gctcgcatat tctgaaaaag attaagccgg tgcataaact gcacagccgc aacaccgaac
    60421 aagccgcgtt tgtggtgttg aaatcgccgt cggttccttc ggtgctggtg gaaacctcgt
    60481 ttatcaccaa cccggaagaa gagcggctgt taggcacggc ggcatttcgc cagaaaatcg
    60541 ccacagcgat tgccgaaggc gtgatcagtt atttccactg gtttgacaac cagaaagcac
    60601 actcgagaaa gcggtaagtg atgaaacccg acgcacacca ggttaaacag tttctgctca
    60661 accttcagga tacaatttgt cagcagttga gcgccgtcga tggcacagaa tttgtcgaag
    60721 atagctggca gcgcgaagct ggcggcggcg gacgtagccg ggtgttgcgt aatggtggtg
    60781 ttttcgaaca ggcgggtgtc aacttttcac atgtccacgg tgaggcgatg cctgcttccg
    60841 ccaccgctca tcgcccggaa cttgccgggc gcagtttcga ggcgatgggc gtttcactgg
    60901 tagtgcatcc gcataatccg tatgttccca ccagccacgc gaatgtgcgc ttttttattg
    60961 ccgaaaaacc gggtgccgaa cccgtctggt ggtttggcgg cggcttcgac ttaacccctt
    61021 tctatggttt tgaagacgat gctattcact ggcaccgcac cgcccgtgac ctgtgcctgc
    61081 catttggcga agacgtttat ccgcgttaca aaaagtggtg cgacgactac ttctacctca
    61141 aacatcgtaa cgaacaacgc ggcattggcg ggctgttctt tgatgatctg aacacgccag
    61201 atttcgacca ctgttttgcc tttatgcagg cggtaggcaa aggctacacc gacgcttatt
    61261 taccaattgt agagcgacgt aaaacgatgg cctacggcga gcgcgagcgc aatttccagt
    61321 tatatcgtcg cggtcgttat gtcgagttca atctggtctg ggatcgtggc acactgtttg
    61381 gcctgcaaac cggcgggcgc accgagtcta tcctgatgtc aatgccgccg ctggtacgct
    61441 gggagtatga ttatcagcca gaagatggca gcccggaagc ggcgttaagt gagtttatta
    61501 aggtcaggga ttgggtgtaa ctccctcccc cccattcccg catccgctga tgcagcgtca
    61561 gtgacggctt ctcggcaaac agctgctggt aatccgtggc gaattgcccc agatgccaga
    61621 atccccactg catggcggcg tcttttaccg ttgtgctttg cgaccaaggg cttatcagtt
    61681 cgcggcgtac ggcgttcagg cgaatgcgtt tcagccacgc gtttgggcca atgcctaaaa
    61741 tagcgtgaaa cgcgttttgt agcgtgcggc ggctgacatg cagttgatta cacaagtcca
    61801 gcaccgtcac tggttcggac atgttttcca gcacatattc acgggcgcgg gaaagcaatc
    61861 gacggtaact ctgatgactg atgctttccg ccgtcaccat tggctgcgct tcttccagca
    61921 tcgcccccat cgccattagc aaattatccc ccagcacttt tcgcactgca ggctgatgga
    61981 gattttccgg gttttcgcaa aacgtcgcca gcgcctgttg gacaaagccc cacagagcgg
    62041 ctttatgctg ctctttcact tccaacgccg actggctacg cagcatatgt aatacccgat
    62101 ccggattatg caaaaagtta gcctgacgag tgatgacatc ttcagaaagc actacgccaa
    62161 ggatcgtgta atcgtccggc gtacttaatt caaactcggt tccaccgggg cgcgtcgcta
    62221 tttctgcgct tcccagacat tgcgaaccga taaatccctg ctcaccgcgc gtcgccggaa
    62281 tgccaaacca gaacgagttc ggccagacca ggcacgactg gcgcagcgcc agaccggtgt
    62341 attcacggaa aacctgaata tcatcgagta gaatttccgt aaattcacca tgaaacttgc
    62401 ccggatgcag ctgatcgtaa atctgctgcc aggcggtaat tgtcaaagca tgttcataga
    62461 catccgttgt ctgtcgttga tgaacattat ccacttcgac cttcggcgtg agcttcaggt
    62521 tttcgggtaa gggttcatga taaagatggt gcaaattggc tgtacgggtc tttttcatga
    62581 tgttaatgcc gggtgttgta ggacacccgg cacctccgac aggttaatgg ggcttgagac
    62641 gataacgact actgcgttta cgtaacgtcc cggcagaaaa gagctgttcg agcgcatttc
    62701 tggctttttc cagcggccag ccaaagtggg cagccacttc tcctgccgtc attccctgac
    62761 gtactgatgt taacagcgcc aacaactcgt cagcagattc cgcgacgata actggcgcgt
    62821 cgggttcctt tacgggttgc ttaggctggc ggttaaagcc agtaaccagc cactgggtgt
    62881 catcgtctgg ccgaccgatc tccttacggc tgatgacacg cccggtacgc atcgcggcag
    62941 cacaacctgc gtccagcgcc gcacgacacg ccgccagatc gccttccacc accagcgtta
    63001 ggcgaccagg gtcaagcact tcgtgactga gcagacgaac gttagctgct ttgagcatgg
    63061 catcagctgc atctatcgcg gcgaccatgc cgtccacttc cagcaatccc agtgcattga
    63121 tcatcggcaa cctccgttac gcacgctgga ttggattacg cgcgatttcc agcactgcat
    63181 cggtaaaggc gttacaggct gctttacacg cggcctggct accggttaaa aatgctgccg
    63241 agtagttggt ttcagacggt ggcgggacat aggtcaccag ctgtacgtcg gcagatttca
    63301 gggctgcatc aatgccgtag gtcgcttcca gcggcggtgc caccagatac gccatcggat
    63361 cgccaagcgt gatcccggcg gttgatgaga gataagaacc ggtacgcgaa actacatgtg
    63421 ccaggaaggc tgtgttttct gcgtcattag cccactggaa agcagcgcca ttttcaatat
    63481 gcgcaaccat cgcatccaga ccagcacgca cttccgccgg gtttggccca ccaagcataa
    63541 tcagcacttc accggcagtc ggtgacgggc cgtgtgcggc tcccgcatac agcgagcggc
    63601 catacaccac ttccaccatc gcctgcttgg tcgcttcgtc agccgcaatg taggtgacgt
    63661 catctgaatc agcagaaatc agcccgaggc tacgaatatg cggcggcaat ttcagttcac
    63721 gcgcaaattc ggcgttaacg gaggcaatca cccgcatggc ggttaccgac ggtcgaatca
    63781 aatctaaagc tggcatgatg cctccttatc gggtcatgtt gatgccggat gctttctgct
    63841 ccagcatacg tttcgccaaa tccacaatga cggctgcggc ttcaaccggc ggcgtgccgc
    63901 cctggtgaat gttagagata caggtacggt cggcctcaac ggtggttgcc atacgcggcg
    63961 agtaaacggc gtagcaggag aggctttctg actgccctaa tcccgggcgt tcccccacca
    64021 gtaggatcac tactttcgcg ccgaggatct caccgatctg atcttcaatc ttcacgcgac
    64081 cataacgaac aaagaatggt gtaccgactt tcagcccggc ctgtttcagg cccgccatca
    64141 gcggtggcag gatctcttcg tagttcacgg tgatcgcatc agttgacagg ccatcagaaa
    64201 taacgacctg tacgtccgga ttagcaacac actgtgcttt cagcgcttca acagcttctg
    64261 cacacaggcg gcggcccata tccggacgag tcaggtagag atttttgtcg ctaatttcag
    64321 agcggacttc cagcaagccc tgcgctttca cccactcttc cggtacttct ttcagtacgg
    64381 tatctttcga acgagagtga tcggccagga aacgcagcaa cgcctgggta cgcggacgcg
    64441 gaccggcacg accggtacaa acgcgggcca cggtgctgcg acgcagttct gttaatacgt
    64501 ctgcgcgatg cgggttttcc acaccaatcc acgcttttgc ttccgcagaa cctaaatcca
    64561 gcgcgcagct ttccgaggtc actggtgtcg tacaggtggt ggtggcgcac tttgcttctg
    64621 acggcgcggg ggccgcttgt cccattgacg ccatcacgct gcgtacaatt tcttcaatct
    64681 gtttttgatc catgatgtgt tatctccgcg tcatcagaag aacagtgacg gatcgcccgc
    64741 ccgtttggtc aggcgaccgt ttgccataat gcccatgctt tccagccagc gttcaaactc
    64801 cggtgacggg cgcaggttga gtaactgacg cacagtggcg gtgtcgtgga aggccgtagt
    64861 ctggtagttg agcatgatgt catcacccag cggcatcccc atgatgtagt tacagcctgc
    64921 ggtggcgagc aggatcatca ggttttcgtt gaggttctgg tcagcgtcag cgtggttggt
    64981 gtagcagcag tcacagccca tagagatgcc gctcagcttg cccataaagt gatcttctaa
    65041 gcccgcgcgg ataatctggc ggtcgttgta gagatactcc ggcccaataa aaccgaccac
    65101 ggtgttgacg ataaacggat cgtaatgacg cgccagccca tagttacgcg cttccatcgt
    65161 cacctggtca gcgccgaagt tagcgccagc ggacagcgcc gaaccttgtc cggtttcgaa
    65221 gtagaggcag ttttccccgg cgatacggtt aaactctgca cccactgcgc gcgcttcgtc
    65281 gagcatcgcc agctccacgc caaactcttt cagccctttt tcgctgccgc aaatactctg
    65341 gaaaatcagc ccgcccggtg cgccgcgacg aatcgcttcg atctgggtgg tgacgtgcgc
    65401 cagtacgcag ccctgagttg ggatgttgaa tttgtcgatc acgccataga tggtatccaa
    65461 cacgcggctt aagttttcca cgtcgtcagt caccgggtta acgccgatca ccgcatcgcc
    65521 caccccgaag gaaagccctt cgtagatttg cgcggcgata ctttgcacat cgtcacgggt
    65581 atcgtttggc tgcaagcggg cgctaaaggt gcccggaata ccgatggtgg tattggcctt
    65641 tttgatcacc ggcattttct tcgcgccgta gatcaggtcc gcgttggagc aaatcttcgc
    65701 taccgccgct accacttccg aggtcagccc tttgcgggta aaggcaatgt cgtccacgct
    65761 ggtttcatcg ctcagcacat actcacgcag ttcgctgatg ctccagtttt taatctggtt
    65821 gtaggccgtt tcgttaacat cgtcctgaat cagccgcgtc acgcagtcat cttcataggc
    65881 aatcaccgga ttattgcgga tgtccgctac ggtcatttcc gacaacacct gctttgccgc
    65941 cacgcgctcc tgtgagcttg ccgcagcaac gcccgccagc acatcccccg aacgcagttc
    66001 gttggcttta gccagcacct cttttacatc cttaaactga tatacattgc cgaacaatgt
    66061 ggtctttagt ttcataagtc gttccctcag gaaggaaatg cgagtgattt caccgtcacc
    66121 ggcacaaccg atccgccaaa aagaggcgta ccaatgtcga tatagtcccc cgcgcggaca
    66181 atcacttcgt caatgactgc caacgggagt tgttgtagct gtgggcgcaa caacatgccc
    66241 agagctttac caaagtcctg cccggccacc accagcaggg gatgcggatt cggaaaacgc
    66301 gcgacgaaat cgaccaacgc gttgatgacc gtcagtaccg cggcgtaacg cacaggcagc
    66361 gaggcgggaa gcgccagcac gtacgcgtca gttttaggat caagatccag ctgaatcagc
    66421 gcctgttgcc aggcactcac cagatccttt tcatcaattg ggatcgccac cggcaagttg
    66481 cgcagcggca gttgtacgcc ctccagccag attgtgctgc cagagagcga aagggtatgt
    66541 gcacccgcgc caatcaccgt ggcgcgcacg gtttgcgccg gaaactgcac gttcatctca
    66601 cgcaggcgcg gatggtcatg cagcgccgtt gccagaagcg ggccaatatc ggcaaaacag
    66661 aacgggtcgg cgggctggtg gcgataacat tcgccaacgc cgccagaaag cgtaatgatt
    66721 tcgggcgtaa cacctacggg cagcaaaccg gtttgcatca atgcctgcgc gagcggcgaa
    66781 agcgttccgt caatcacttc gacaatcagc tctgccatcc gccgggtaac ctgcaccagc
    66841 tgcgcgccgg tcagcgaacg ggcttcggtg cctgcaccga agcactcatc cacaatcatc
    66901 tgccccggtt tatgagcgta aaccacacgc ccctggctgt cggtttccag caggcgaccg
    66961 ccgacattga ggcaggcggt gccgctgatt tttccggcat cgaacagggc gtagttcgcg
    67021 gtgccaccgc cgatatcgat attcaggacc cgacacagcc gttgttcaga aagggtttgc
    67081 gccccggcac cgtgaccggc gatcacggat tcgagatgcg gtccagcgct ggcgacgaca
    67141 aaatcgccca gcgactggga gagcgtcatc accgccgggc gagcgttgcg ggttttcgcg
    67201 ctttcaccgg tgatgatgat ggcaccagaa tcaacgcttt ccggcgcaat gcccgcagcc
    67261 tgatattgct cgaggattaa ggttttcagt tccgcttctt ttaaaccacc ctgtttatcg
    67321 acaggggtaa agaacaccgg gctttgccag ctaatttcgc gtttgatgaa ttcgtagcgc
    67381 ggcacctgcg acaccgccgc acggttaacc agctccaggt gggagaaaat cacctgggtg
    67441 gtggtggtgc cgatatcgat accgacgctc agtagctggc gagtgttcac gattgcgcct
    67501 ccgcttcggt tttagccgcg ctcgcgtctt ctttaggtac cagcatcatc gccacgccaa
    67561 tcgccgtgac gccgccgatc agcttgccga caatcatcgg gaagatcatg gcgttcatgt
    67621 tggcagccgc gaagcctaaa tggtcgccca gggcgaaagc agcggaaacg gcgaaggcac
    67681 agttgatgac tttgccgcgg gtatccatct gcttcatcat gccaaacatc gggatgttgt
    67741 tggcaagcgt tgccaccatg ccggctgccg ctatattgtt catattcagc accttaccga
    67801 cactcatcag cggtttttca aaccagcgag tcagcagcag caccatcgga tacgccccca
    67861 gcagcacgca ggagatagaa ccgataactt caatggcgcg catcacttca ccgggtttgt
    67921 cgcctggagc catgaagatc ggatccagac cggggatcag ttcccaaccg agcaggaatt
    67981 tcactaccgc agcggcaaga ccgagggtga tcaatgcaac gaggaatttg gcgaagatct
    68041 ggaagccgtt gatcattttt tccgggatga atttcagccc cagcgccacc agcaccgcaa
    68101 cgataagtac cgggatcatg ttcatcagga tcagcgcaaa ggtgaattcc accggctgac
    68161 cgttgatctg cacaccggag tacatagcaa ccagaccacc agcaatacag ccaatcggaa
    68221 tggtcacaat gcccgccagc acgccgagcg ccagataacg acggtcagaa ggttcgataa
    68281 tgccgagcgc caccggaatg gaaaacacaa tcgttggccc catcatcgac ccgagaatta
    68341 acccagagta tagccacgct gctacgtcgc cgcccgccag ctctttggcg aggaagaagc
    68401 cgcccatatc gcacgccagc agtgttccgg cgaacatcga tgggttagcg ccgagcattt
    68461 cgtaaaccgg gataatcacc ggcccgagta cgtgggccag taccggtgcc agcgcggtca
    68521 taccgaccat agccaggccc aaagcgccca ttgccataaa gccttcttcg aactgaccgc
    68581 cggatccttc gatactttta ccgaacttac cgaggaaacg agcagaaccg ccgaactgcg
    68641 acaggatcct gtctacggca gctatcagca taaagaacat catgatgtac atgatgattt
    68701 cgttaatacc catcgccttt actccctgtt agttgttatt tattggcgga tgcggcgtga
    68761 acgccttatc cgccctacat gtgcaatccc gaaggccaga taagacgcgg caagcgtcgc
    68821 atccggcaat tgcaccgcgc cactggcgga tgcggcgtga acgccttatc cgccctacat
    68881 gtgcaatccc gtaggccgga taagacgcgg caagcgtcgc atccggcatt tattgcgccg
    68941 ctgcgtacag gccgacaatc tgctccaggc tggcggtacg cgggttactg cgcagacaaa
    69001 tatcttccag cgcggcctgc gcccatgcgc cgtaatgcgc agatgtcgca ccaacatcgc
    69061 ccagtcgttt accaatccca acttccgcta tcagctcact taccgcatta atagcgtcac
    69121 gatcgtcgga ttttttagtt cgcagcgccc gaccaaccaa gctaaaccga tcacgacaaa
    69181 ccatccggtt aaattccatc accgttggca acaacatggc gttcgccaga ccgtgcggaa
    69241 tatgcagcgc cgcccctggc tgatgcgcca tcgcgtggca caacccaagt ccggcactgg
    69301 aaaacgccat tcccgccata catgaagcca gcaacatgct ctcgcgcgcg gcaaggtcat
    69361 gaccgtagcc caccgctttc ggcagcgatt tgccaatcat cgcaatggca ccaatcgcca
    69421 gactgtcggt aaacggtgtg gcgttcagcg cgctgtacgc ttcaatggca tgggttaacg
    69481 catcaatgcc agtcatcgcc gtgacatgcg acggcacacc ttcggtcaat gcggcgtcga
    69541 gaatcgccac atccggcatc agcgaggcat gggctaacac ctgcttgcgc ccgctcaccg
    69601 cgtcgataat caccgttacg ttggtggttt cagagccggt tccggcggtc gttggaatgg
    69661 caatcaacgg caggcgcggt tgcagaacgc tggtttctga catttctgcc agcgtgctat
    69721 cggggttcgt caccagcaac gccacggctt tcgccgcatc cagcaccgag ccgccgccaa
    69781 acgcgatcac cccgtcacaa cctgattcac gcaactgcgc cacggctgca cacacgtcgg
    69841 tgatgcacgg ttcgcccacc ggacatggcc agagcgtcat ggcgatacct ttaacggcca
    69901 ggctacgcgt cagcccggcg gtcatccccg cctgatgcaa aaagctgtct gccatcacga
    69961 acagatgttt cagcccacgc gtttgcgctt gctgcccaca actgctcacc gcgcccggac
    70021 cgcaaagcgt caccggtgga acgctaaatg ttttcacccg ctgcagattc agggtatcga
    70081 atgcctgaaa aagcgccgtt tgcaattcac tttgcataga tcccctccgt tttctctcgc
    70141 ccactactgg cgatcgccag cggggtcata aacaaactgt gctgcggtaa atgcacctgt
    70201 aacgccggga attgtttgcg aaacagttcc gctacgcccg gttgcataca ggaaccgccc
    70261 gccagccata aatccgtaat gccctgccct tcaatatgcc gggccacaat gtccgccatt
    70321 ttttcgtaca ccggtttcac cgcgggccaa atctcgtcac cgtgaccgcg tttgtactgc
    70381 tctgcctctt ccagcgaaat acggcgattt ccggcgagag tcagagagat gtgatgtccg
    70441 ccggtggctt catccgccga gtacgtcact ttgccctttt tcacgatggc aatgccggta
    70501 gtgccgccgc cgatatccac tacaccggcg ttgtccagtt gcagcagatc cgcaactgcg
    70561 gttggctcat ccagcacatg gctcacttcc agtccagcag actccagcac gttgatggaa
    70621 atacgcgggt ccgtacctgg aggaaaagag gtcgccgcat ggctaaaacg gcgaccgaat
    70681 tgctgctcga gggtgtcgag atggcgacga acaatggtga ccgcaccgaa gaaatcccag
    70741 acgatgccgt cgcggaccac atcagcccag tcgaggcata ccgccaccgg ctgaccgtcg
    70801 cggtcgacaa ccatcgacac cacatcgcag gtgcccagat ccacccccag ccacagcgga
    70861 gattccgtcg cggcgggcgt ctggttacac agcgttgccg ccgtttgcag acgtggggtg
    70921 agccattgtt cgtcgtgcgc catctgttac tccttaaaca atgcgaaacg catcgactaa
    70981 tacacagcga cgcagacgga caaacgtccg cgcgctggtt accccttcac cggttggcgt
    71041 ggtgatggtc atagtggtcc agccttcccc gcccagcccc agcccggcaa tgcacggtcc
    71101 gttcttaacg aaaatgctgg tatcaatggc attcgccatc tggttcatgt tctcgatatt
    71161 gcgcgagtgc attgccgccg tgtggtggca accgccttcc agtttcaccg ccagcgcaat
    71221 ggcatccgcc acgttggcga cgcgcacgac gggcagcacc ggcatcatca gttcagtcac
    71281 agcaaacgga tgttctgcgg tggtttctac aaacagcagt cgcgtttgtt ccggaacttt
    71341 aaggccgatt gccgccgcga ttttcgccgc atcgcgacca acccagtcac ggctgacggt
    71401 gccttttccg cgctcgtcga tatttttcag caacaccgat tgcagttgct gcgcctgctc
    71461 tgcggtcagt ttcaccgcat gctggccttc catcagacgc atcagttcat cggctacgct
    71521 atcaacaaca atcagtacct tttcgtcggc acaaatgatg ttgttatcga aagaagcacc
    71581 tttgacgatg gactgagctg cacgggcgag gtcggcggtt tcatccacca ctaccggcgg
    71641 gttaccggca cctgccgcaa tcaggcgttt attggtgtgt ttgcgcgccg cttccactac
    71701 cgcttcgccg ccggtcacca ccagcaggcc gatacccgga aacttgaaca agcgttgcgc
    71761 ggtttcgata tccggatttg ctacggtaac cagtaagttt tccggcccgc ctgcggcaac
    71821 aatcgcctgg ttgagcagcg taatcgcccg ctgggagact tttttcgccg ccggatgcgg
    71881 ggcaaaaatg acgctgttgc ccgcggcaat caggctgatg gcgttattaa tgacggttgc
    71941 cgccgggtta gtggaaggcg tcaccgaagc cactacgccc cagggcgcgt tttcaattaa
    72001 agtcaggccg ttgtcgccag tcagcacctg tggagagagg cactcaacgc ctggtgtgcc
    72061 gcgcgcctga gcgacgtttt tggcaaattt atcttcaacg cgccccatgc cggtttcact
    72121 gacggcaagt tccgctaaat ctctggcgtg tttttcgcct gcttcacgaa tggcagcaat
    72181 ggctaactgg cgcattgcca cgctttttaa cccttgctgg gcgactttgg ctgccgcaac
    72241 ggcgtcatcc agggacgcga aaacgcccat ctcatgaacg gcggcgggcg gcgtgtcact
    72301 gctttgcatt ttcagcagta ccgctttcac cacctgttca atatcctgtt gattcatgat
    72361 gttctgcctt atttgtggaa aatgacctga ccgccagaca ccacctcatc gacgatgcca
    72421 atcacgcaca gatcgaccgg tgacgtttcg cttttatgcg cctggcgggc ggaactgcca
    72481 ctcaccagca acacccactc cccggttccc gcgccaatat tgtcgatggc gacggcgcat
    72541 tgcccgtcag gattaccttg tggatcaatc atttccacca tcagcaattt gtcatgcgcc
    72601 agtccgtgat ggcgtacggt acaaacaatt tgtccagtga cgactgccag tttcataccc
    72661 gcctccgtgg cgtatttcag gtaaaagctc cccctaccct ccgcagaagg taaaatgaaa
    72721 aaggagagag cgtgacgccc gaatcgacgt cacacagggt gattacaggt tgctgctatc
    72781 gcctttcagg ccgatcggga agacttcttc caggtcgccg tgcggacgcg ggataacgtg
    72841 tacagatacc agctcgccga tacgctgtgc cgctgcggca cctgcatcgg tcgcggcttt
    72901 acaggctgca acatcaccac gaaccatggc agtacacagg ccgccgccaa tctgcttaac
    72961 accaaccagc ttgacgcgcg cagctttaac catcgcgtca gaagcctcaa tcagtgcaac
    73021 caggccccgg gtttcgatca ttcctaatgc ttccattgtg ctttcctctt tatcagggtc
    73081 cagaacggga ccgttcattc aaccagtgtt tgtaaactgc tttcgcggtt cacttctgtc
    73141 tgacgctgca cagctgccac cagcgccagt tcgataattt cctgcacgct acaaccacga
    73201 gagagatcgt gcatcggcgc ggcaagtcct tgtatcaatg gcccgacggc acgatatccg
    73261 ccaagtcgtt gtgcgatttt gtagccaata tttccggctt ccagcgacgg aaaaaccatc
    73321 acattagctt tgccctgtaa cgggctggca ggcgcttttt gcgccgccac ttccggcacg
    73381 aaggcggcgt caaactgcaa ctcgccatcg acaaccagct ttggtgcgcg ctcacggacg
    73441 atttctgtcg cctgctggac gttggcaacg caggggtgac gggcactacc gttgctggaa
    73501 aacgacaaca tcgccacgcg cggttcttct ccggtgatgg cgcgccaggt ttcggcgctg
    73561 gcaagcgcga tatccgccag ttgcgccgcc gtcggctgtg gcactacgct gcaatcggca
    73621 aagcccaatg cggggccact gtactgtggc agcatcagga aaatggagga gagcgtttta
    73681 cagcctggct gtaagccgat aatgcgtaat ccggcacgca gcacattcgc cgtggaagag
    73741 agattgcccg cgatacagac atccgctgtc ccggcgctga ccattgcggc agcgaacatc
    73801 agcggatcgg tgagtttatc cagcgcatcc ggcggcgttt tttcgcccgc acgggccagc
    73861 cagcggagag caaattcttc ccgcattgcg aggttgccat gcggatcaat cacctgtagc
    73921 ccgtccatcg ccacgccgtg actgagcgca aactgacgaa gttcaaacgg attggcgacc
    73981 agaatgggcg ttgccagacc ttgttgatgt aaatattgcg ccgctttcag cacacgttga
    74041 tctaacgcat ccggaaaaac tactctggcg ggcgctcgca acgccagttt acgacaacgt
    74101 tcaataatca ttgcgtttcc cccagtcgtt gttggatctg cttcaccgtt agcgactgct
    74161 tcgttacgct caggatcatc atcacgtaaa ccgtgctgga aaggcggttc agcgcctgca
    74221 agatgtccgg gcgtaatact tcaaagctgc gggtgataaa cacctgcgcc gctacagttt
    74281 cggtttcccg cactttggtg cgtagcagat tgagcaacgc cgcatcacgc ccgtggctgg
    74341 cttccggcac cagatgatcg tggtcgagat agcgcagcgg ctggtgagaa agccgatgca
    74401 gatcttcgtc agaaagcccg acaatcgctt gttcgcccag cggttccccc agcgcatcgg
    74461 cgcgcataat gttgccaaga cgcgaacgga tatccgccag ccacggctgc cacggttccg
    74521 ccagttcaat ttgcagccac actgccagcg caatggtgct gtcgagaaca gcgcgaaaag
    74581 ccaggcgcgg atcgcttttg gcaaccattt tttccgccga caggtgagtc agcgtatccg
    74641 gcttttttgc caccggctgg cggcacagtt cgcagcacgc ctgcggatgt tcatcgctac
    74701 tggttagccc gtgaacgggc tgcggctgct gctgttcatc gtcaacaaac aggcggccct
    74761 gctcgtcaat aaacttgatg cgcagatggc ggctttccag taactcccgg gcagagggcg
    74821 tcaggcgact gtccgcaggg agatggatct ctgcgccttc gctgagcgta tgattcgctc
    74881 ttagccatgc ttcggtgatg aaatctttca taaggattgc cagttagccg gccaggcgac
    74941 atacaggaat ttcacgctgg atgtcgtacc aaattcgatg ctggagcctt tcgggataaa
    75001 catcacatcg cccgctttgg cgatcatagt ttcgccttcg tggcgaacat gcagctcccc
    75061 ttccagcacc atgtcgattt cgtcgtagtt cagggtccac ggaaagaagg cgttctccca
    75121 ctgcatgaaa ccagcggcca tactgctgcc gtcgtctccg gtcacgagat cagtcaagcc
    75181 tacgcagtgc ggttccgcgc catcgaaacg accaaacttg acgctgctgc catcaataac
    75241 tttgatgccg cctttgccgg tcaccgattt aaagctcggc tgcagtgcac cctgctccag
    75301 cgactgtttt tccttcatca ctttttccat cagctgcgcg accaggcttt cggtaaactg
    75361 accttccggc agttgggcga taatggtttc acggatgcgc tggctttcgg ttttatcggc
    75421 tgatgcgctg gcgggaacag acgccgttac cgggatcgat tcatcacatt cggtgatggt
    75481 aaaacccaac agatcagcca cttcacgagc ttccggggta ataatgctgg cgcgcagaac
    75541 cacggacatt gccagttcgc cgcgcgcgtg tgcttcacga atatcattcg ctgtgatgag
    75601 ttttttcacc tgcttcctcc tgtttggtga gagatgccag ataatccacc agttgctgca
    75661 cgctttgcgg gtcgtggctg tttagctcga aaatcggctc ttcgaagccc gtctcaagca
    75721 ataactttcg cgttgcggcg acgtcagcat ctggcatgtc cgttttactg atgacggcga
    75781 tttgccgttt gctgacgcca atatccagca acccggcagg caaccgactt tccgggtcat
    75841 tcgcgccgtg gacatagatc agcatgtcca catcctgtag cgtggtaatt aatgcgtgat
    75901 accagcgggg atggctaaaa tattcacccg gcgtatcaat atcgccatta tcattaaatt
    75961 ccacggcctg cgtttttctg gcgagggtat aattcccctg caacgcatta aataacgttg
    76021 tttttcctgc cccgacggta cccacaaacg caattcgttt catgaatgcc tcatattaac
    76081 ttttggtcat ttcacaaagc gtataattta ataagcgtcc taaaccactg acggtttgtg
    76141 ataacgcttc ttctactgcg cccaccgaac cataaatcac cagcgcgccg ctaaaacggt
    76201 cgagaaaacc gatatgcaca tcggcggctt tcaacgctaa atcaccggcg atcatcgccg
    76261 tttctccggg agttagcgtc ataatgccaa ttgcgcccgc atccggaacg ccgattttct
    76321 tcgccagttc ctcgccggga tgcgcgatga gatgcgccag cgtgacctgt ttgccgggca
    76381 caaattcctg aatgatgcgt tctttatcca tgagtcgccg cctgtgaaaa actttcgtgt
    76441 atcgtgacaa tttttcgtca gagtaaataa caaaattcgg caagcgcgtt taaaaggtga
    76501 ggtagatcac taagaatgga gagatattta ctgttatgaa taaactttca tatgcgacgc
    76561 atgaatataa ataaaataat atcgagatat tttatatact gattttttta ttattcgtaa
    76621 actcagtaat aagctttact aatatattgc agctaaactg cttaccctga atattcaggg
    76681 taagcgtgag agttaaaaaa aaattacagc ggttgggttt gcgcttcaac cacggccagc
    76741 gccaccatat tgacgatacg acgtaccgat gcgatcggcg ttaacacgtg aaccggtttc
    76801 gccacaccca tcagcaccgg accgacagtc acgccttccg agctggaaac gcgcagtaag
    76861 ttgtaactaa tgcgggcagc ttccatgttc ggcatcacca gaatattagc ggaacctttc
    76921 aaagggctgt ccggcatacg gtcattccga atcgcttcca ccagcgctgc atcgccgtgc
    76981 atttcaccat caatcatcag ttctggtgca cgttccctga ccagttccag cgcctgacgc
    77041 attttgctcg acgacgggca gtcagaagaa ccaaagttgg agtgcgacaa cagagccaca
    77101 cgcggctcaa taccaaaacg acgaacagtt tctgccgcca tcaaggtgat ctccgccagc
    77161 tcttctgcat caggctcatc attgacgtag gtatcggcaa taaaggtgtt accactcggc
    77221 aacagcagcg cgttcattgc acctgcggtg tgaacgccat cgcgataacc aaagacattt
    77281 ttcaccacgc taaagtgttc atgataatcg cccaccgtac cgcaaatcat tgcatcggct
    77341 tcgccacgct gaaccatgat cgcgccgatc actgtcgggt tactgatcag cgcacgctgc
    77401 gcctgctcct gagtgacacc gcgacgcttc atgatctgga agtattcggt ccagtactct
    77461 ttaaagcgcg gatcggattc gttattgacg atctcaaaat caacgcccgc cttgatctgc
    77521 aagcccagtt tctgaatgcg catttcgatc acgttcggac gaccaataag gatcggtttc
    77581 gccagtccca gcgttaccag ttcctgagtg gcatgcagaa cgcgcgcctc ttccccttcc
    77641 ggcagaacta cgcgcttcgg cgctttgcga gcctgggaga aaatcggctt cataaacagg
    77701 ttagttttgt aaacgaactc cgtcagcttg tcgatgtaga cgtcgaaatc agcaatcgga
    77761 cgcgtcgcca cgcccgactc catcgccgct ttcgccaccg caggagcgat cttaacgatc
    77821 aagcgcggat caaacggttt tggaatgatg tattccggac caaagctcag atcctgatcg
    77881 ccatacgctg aagccaccac ttcactctgt tccgcatggg ccagttctgc aatcgcgcgt
    77941 accgccgcca gtttcatctc ttcgttgatg gcggttgcgc caacgtccag cgcgccacgg
    78001 aagataaacg ggaagcaaag gacgttgttc acctggttcg ggtagtcgga acggccggta
    78061 cagatgatgg catccggacg cacttctttc gccagcggcg gcagaatttc cggttccggg
    78121 ttcgccagcg ccaggatcat tggcgcacgg gccattttct tcaccatttc ctgggtcagc
    78181 actttcgggc cggaacagcc gaggaaaata tccgcgcctt caatcacatc atcgagggta
    78241 cgtttaccgt catccaccac cgcgtatgcg gctttggttt ccgccatgtt tggctcacgg
    78301 ccccgataga taacgccttt tgaatcgcaa accacgatgt tatgtttttg cagacccagc
    78361 gctaccagca ggttcataca ggcgattgct gcggcacccg cgccagaaac caccatccgc
    78421 acgtcggaga tgtttttctc caccacgcgc aaaccgttga ggatggcggc agtgctgata
    78481 attgccgtgc cgtgctgatc gtcgtggaat accggaatat tcatccgctc gcgcagtttc
    78541 tgttcaatat agaaacattc tggcgcttta atgtcttcga ggttgatgcc gccaaaggtc
    78601 ggttcgagcg cagcgacgac ttcaataaat ttgtccgggt cgagttcatc aacttctatg
    78661 tcaaatacat caatcccggc gaatttctta aacagaacgc ccttgccttc catcaccggt
    78721 ttgcctgcca gcgcgccaat gttgcctaac cccagcaccg ccgtaccgtt agagatcacc
    78781 gctaccaggt taccgcgcgc ggtatatttg taggctttta acgggtcttt ttcgatttca
    78841 agacaaggtg cggcaacgcc tggtgagtag gccagcgcca gatcgcgctg cgttgccaga
    78901 ggcttggttg gagaaacctg gattttccct ggaactggaa attcatggaa atcaagtgca
    78961 ctttgtttta actggtcatc catttggttg ttcctttcac gtaacgttca caaataaagt
    79021 gtgtgggcaa cagcccctgc ccacaacgtg gcgcacatta ttaccttgcc ggagtctaca
    79081 gactttgagc aagtccaaac tctcaccatt aatataatgt tttggtaata atcctataac
    79141 actgatgtta cctgcttaat ccagcaatac catgcctgtc tgctatgctt ttttgatgcg
    79201 tttagcgaaa tttctcagaa gtgtgaatta acgcactcat ctaacacttt acttttcaag
    79261 gagtatttcc tatgaacgag ttagacggca tcaaacagtt caccactgtc gtggcagaca
    79321 gcggcgatat tgagtccatt cgccattatc atccccagga tgccaccacc aatccttcgc
    79381 tgttactcaa ggctgccgga ttatcacaat atgagcattt aatagacgat gctatcgcct
    79441 ggggtaaaaa aaatggcaag acccaggagc aacaggtggt cgcagcgtgt gacaaactgg
    79501 cggtcaattt cggtgctgaa attctcaaaa tcgtacccgg tcgcgtgtca acagaagttg
    79561 atgcacgcct ctcttttgat aaagaaaaga gtattgagaa ggcgcgccat ctggtggact
    79621 tgtatcagca acaaggcgtt gagaaatcac gcattctgat caagctggct tcgacctggg
    79681 aaggaattcg cgcggcagaa gagctggaaa aagaaggtat taactgcaac ctgacgctgc
    79741 tgttttcttt tgcacaggcg cgggcctgtg cagaagcagg cgtttttctg atttcgccgt
    79801 ttgtcgggcg tatttatgac tggtatcagg cacgcaagcc gatggacccg tatgtggtgg
    79861 aagaagatcc aggcgttaaa tcagtacgca atatctacga ctactataag caacaccact
    79921 atgaaaccat tgtgatgggc gcgagcttcc gtcgcaccga acaaatcctc gccttaaccg
    79981 gctgcgaccg actgactatc gcaccgaatt tgctgaagga gctacaggaa aaagtttcgc
    80041 cagtggtacg taaattaatc ccaccttctc agacgttccc acgcccagct cccatgagcg
    80101 aagcggaatt ccgttgggag cacaatcagg atgcgatggc ggtagaaaaa ctgtctgaag
    80161 gcattcgtct gttcgccgtt gatcaacgca aactggaaga tcttcttgcc gccaaactat
    80221 aaaccagcca cggagtgtta tatgtcccga aaagaccttg ccaatgcgat tcgcgcactc
    80281 agtatggatg cggtacaaaa agccaattct ggtcatcccg gcgcgccgat gggcatggct
    80341 gatattgccg aagtgctgtg gaacgatttt cttaaacata atcctaccga cccaacctgg
    80401 tatgatcgcg accgctttat tctttccaac ggtcacgcgt cgatgctgct ctacagtttg
    80461 ctgcatctga ccggttacga cctgccactg gaagaactga agaacttccg tcagttgcat
    80521 tcgaaaaccc ctggtcaccc ggaaatcggc tatacgccag gtgttgaaac caccaccggt
    80581 cctcttggac aaggtttggc gaacgccgtc gggctggcga tagcagagcg tacgctggcg
    80641 gcgcagttta atcagccgga tcatgaaatt gtcgatcact tcacctatgt gtttatgggc
    80701 gacggctgcc tgatggaagg tatctctcac gaagtctgtt cgctggcggg cacgctgggg
    80761 ctgggcaagc tgattggttt ttacgatcac aacggtattt ccatcgacgg tgaaaccgaa
    80821 ggctggttta ccgacgatac ggcaaaacgt tttgaagcct atcactggca tgtgatccac
    80881 gagatcgacg gtcacgatcc gcaggcggtg aaggaagcga tccttgaagc gcaaagcgtg
    80941 aaagataagc cgtcgctgat tatctgccgt acggtgattg gctttggatc gccgaataaa
    81001 gcaggtaaag aagaggcgca cggcgcaccg ctgggtgaag aagaagtggc attggcacgg
    81061 caaaaactgg gctggcacca tccgccattt gagatcccta aagagattta tcacgcctgg
    81121 gatgcccgcg aaaaaggcga aaaagcgcag cagagctgga atgagaagta tgcagcctac
    81181 aaaaaggccc atccgcaact ggcagaagag tttacccgtc ggatgagcgg tggtttaccg
    81241 aaggactggg agaaaacgac tcagaaatat atcaatgaat tacaggcaaa tccggcgaaa
    81301 atcgctaccc gtaaggcttc gcaaaatacg cttaacgctt acgggccaat gcttcctgaa
    81361 ttgctcggcg gttcggcgga tctggctccc agcaacctca ccatctggaa aggttctgtg
    81421 tcgctgaagg aggatccggc gggcaactac attcactacg gggtgcggga atttggcatg
    81481 accgctatcg ccaatggcat tgcgcaccac ggcggcttcg tgccgtatac cgcgacgttc
    81541 ctgatgtttg ttgaatacgc tcgtaacgcc gcgcggatgg cggcactgat gaaagcgcgg
    81601 cagattatgg tttataccca cgactcaatt ggcttagggg aagatggtcc gacccaccag
    81661 gccgttgagc aactggccag tctgcgctta acgccaaatt tcagcacctg gcgaccgtgc
    81721 gatcaggtgg aagcggcggt tggctggaag ctggcggttg agcgccacaa cggaccgacg
    81781 gcactgatcc tctccaggca gaatctggcc caggtggaac gtacgccgga tcaggttaaa
    81841 gagattgctc gtggcggtta tgtactgaaa gacagcggcg gtaagccaga tattattttg
    81901 attgccaccg gttcagagat ggaaatcacc ctgcaagcgg cagagaaatt agcaggagaa
    81961 ggtcgcaatg tacgcgtagt ttcgctgccc tcgaccgata ttttcgacgc ccaggatgag
    82021 gaatatcggg agtcggtgtt gccttctaac gttgcggctc gcgtggcggt ggaagcaggc
    82081 atcgccgatt actggtacaa atatgttggt ctgaaagggg caattgtcgg gatgaccggc
    82141 tatggggaat ctgctccggc agataagctg ttcccgttct ttggctttac cgccgagaat
    82201 attgtggcaa aagcgcataa ggtgctggga gtgaaaggtg cctgatggtg atcgccggat
    82261 gctatatgtt cccctcaccc taaccctctc cccagagggg cgaggggacg gtccgtgcgc
    82321 tttttagggg cgcatctggg attttgttta acgggtgatc cacaacgtgg gccaggcgtc
    82381 tggcccatgc cagttatcgc aggtgggttc tgcggcgtaa cgcaccaggc gaaaacgctg
    82441 accgtcaaag cgccagcgcg cctgaatgcc acaatcgctt aatccgcgcc ctttcgctaa
    82501 ggtcaccagt tcacgcgatt tttcatcgaa tgttgcgttc atcagttcca gttcattcgt
    82561 ctcctgaccg ctgttgaacg gcaaacgtaa ccgaaccggg cgcgaggcca gtggcttttt
    82621 acgcgacaca atccatgcca aatcaatggt gttataggcc cctgcctcac agctaatcat
    82681 catcagcgct ttatcatcgg tcagcgcagt gacattcacc tctcgacgca atggatcaag
    82741 cgagcagcgc agaccattca tccgccagtt tccataatcc agcaaatcgt tgcgttcttc
    82801 gagtgagagt ggcgtcggcg ttgggttaac caccgcgacc tctttcagcg caggcgcggg
    82861 cggtacgctg agcggcggtt catccccttt ctttatccac gcggtttcac tgccaacgcg
    82921 cttttgctga gcatctataa acaacaacgc cgcttttaag ccactcagag aaatagtctg
    82981 attgccgtcg cgtaaggtga tcgccctccc ttcctgaatc atctgcaaaa acgcggtgat
    83041 ggttgccgta tcatcggtta ctaataacca tggtgaaatc cgccacttgt caccacttaa
    83101 cgccaacggc tcaccatcta acagcagccg tggcgctatc tccccttcag acgcatccgg
    83161 cgacttcaac ccgccgcgct caatacgtaa aacggcatcg gtatgcgccc cggcgctgcg
    83221 gctcagggtc atcaccagtc cgttatgatc gcccgtatta cgcgccacgc agaagttttg
    83281 gttattacag gtgacctgcc agtcggaaaa cgcccgttgc gctggtgccg cccacaccaa
    83341 agacgtcggc aacaaagcga aaaaaaagag aaggaaaatg cgatagcgca tggacggtac
    83401 gaccccagaa gttcaaacaa acagcacaag tcgtatcttc gttgcgcagg tagcgcggct
    83461 caatcggatt tatcggattg agccaataat aaacaacgaa atattattgt tatttttcag
    83521 tccattaaat gtgacatttg caaatagtta agcaataaca ccgtcttacc gtcacgtatc
    83581 tcgccagttt tgatcatctc cagcgccttg ttgaacggca gctcgagcac ttcaatatct
    83641 tcatcttcga caccaccgcc ggcgttagcg cgctggctgt cactgtattc ggcgataaag
    83701 aagtggatta gctcagtcac accgcctggc gacatataca gttcaaataa tttgcgcact
    83761 tcgccaactt cataacccgt ctcttcaatc gcttctttac gaatacacac ttccggttcg
    83821 tcgttatcca gcagcccggc gcaggtttca atcagttgcc cgctttcatt gccattaacc
    83881 caggtagcga cgcggaactg gcgaatcaga accacggtct ttttcttcgc gttgtacagg
    83941 aggatcgttg caccattacc gcgatcgtaa acttcacgtt tatggcggat aacttcgcca
    84001 tctttgcgcg tgagatcgta agtaatgttg tgcagggtga aatagttatc ggagagaatt
    84061 ttgtctttaa tgagggtgat ttgttgcgtc ataccgactc cacagcgcga aatgaacaat
    84121 tatcttacgc tgtgaagccg ggtttgtctg cgcaggctat cagtgagatt tgactgattt
    84181 tacccccagc cagtcaataa tcccctgtgc cgcatgacgt ccttctgcca ttgcggtaac
    84241 caccagatcc gcaccacgca cggcgtcacc accggcgaag attttcggat tggtggtctg
    84301 gtaacggtac tggctttcca catccgcgat gatgcggccc catttgtcta ccgttacacc
    84361 gtgcgactcc agccacggca tcccgtgcgg attgaagcca aacgccataa tcaccgcatc
    84421 ggctggcatg acaaattcac tgccttccac cggcactgga cgccgacgcc cctgggcatc
    84481 cggctcgcca agacgcgtgc gcaggaaacg aatcccgcag acgtgacctt gttcattcag
    84541 ttcaagcgcc accggctgga cgttaaattc gaagttggcc ccctcttcac gggcgttctt
    84601 cacctctttc ttcgagcctg gcatgttagc ttcatcacga cgataagcgc aggtgacgtt
    84661 actcgcgccg tggcgcagcg cagtacgcac acagtccatc gcggtgtcgc cgccgcccag
    84721 taccacgacg ttaagtccgg cagtattgat aaacggctct tccggtagct cttcgagtcc
    84781 catcacctgt ttagtattgg cgatgaggaa cggcagcgcg tcataaacgc ccggcgcatc
    84841 ttcattgggt aaacccgctt tcatggagcg gtaagtgcct acgccaacga agaccgcgtc
    84901 gtattgttcc aaaagcgaat tcaaagagac atctttaccc acttcacaat tgagttcgaa
    84961 gtgaatcccc atcgcgctga agatttcccg acggcgtgcc agcagggatt tatccagttt
    85021 gaaagaagga atgccgaaag tgagcaagcc accgatttct ggatggcgat cgtacaccgt
    85081 cacctctacg ccattgcggg tcaaaacatc cgcacaggcc agccctgccg gacccgcacc
    85141 gataatcgcc acccgcttgt ccactttggt gacatggctt aagtcaggac gccaaccttt
    85201 cgccaacgcc tgatctgaaa tgtagcgttc aatgttaccg atagttactg cgccgtgctc
    85261 atcgcgaata gtacaggcac cttcacacaa acggtcttgc gggcaaacgc gtccggtaat
    85321 ttccggcagg gtgttggtct ggtgagaaag ctcgacggcg gcgtcgatgt ttccggcttt
    85381 caccagttca atccactgcg gtatatggtt atgcagcggg caggtccatt cacaaacgct
    85441 gtgctcgccg cacttaaggc agcgcgaggc ttcccgttgt acctgatcgg cacggaatgg
    85501 cagataaatt tcatcaaaac cggttttgcg cgcttcaatc gccagtttat ctggctcgcc
    85561 acgcgcgggc gttgcctgca tttgctcgac tttactcatt accggcattt cttgcgccgc
    85621 ggtactggca tgccacggtt gatgttcctg acgtgcggta cgcaagcggc gggatttcgc
    85681 catgcctgag agtgcggcgt cagtgaccag ttgcagcgcg tccgctgggc aattctcgac
    85741 acaagcagga ccgttttcgc gccctgcaca aaggtcgcat ttatgcgccg tggcttttac
    85801 ttttcctgcc gcgacggggg tcaggacgat ttgcatcgta ccaaatggac aggccaccac
    85861 gcaggattta cagccaatac acttttgctg attgacctga atgctgtcat caacgtggct
    85921 gattgcgcca ttagggcagc tacgggcgca gggcgcatct tcacaatggt gacaggtcac
    85981 ggcactacgt tgctgctgat gtttgataac cgtaattcgg ggatgaaaat ggtgttggct
    86041 caggacatgt tgctcatcat tgtgagccat gacacaggcg atttcacaag catgacaacc
    86101 cagacattgc tgactgttgg ccataataaa acgattcata acgaccttct tttttggttg
    86161 taaaaacctt attctttata tgagtgttgt tattacccga cttacagggg aatcggcaat
    86221 gttcatgtgc ccagaataag taactatttc gctataaact gtggcagatc aaataatccc
    86281 atcactgact aaattgcgtt tcagcgaatt ggaacattaa tgattttttg tggagaagac
    86341 gcgtgtgatt gttaaacgac ccgtctcggc cagtctggcc cgggcctttt tttacattgt
    86401 gctgctgtcg attctttcca cgggtatcgc tctgctaacg ctggcgagca gtttacgcga
    86461 cgctgaggct atcaatattg ccggatcgct gcgtatgcag agttaccgcc tgggctacga
    86521 cttgcaaagt ggcagtccac aactcaatgc acatcgccag ttatttcagc aggcactgca
    86581 ttcaccggta ttaaccaatc tcaacgtctg gtatgtgcca gaggcagtaa aaacccgcta
    86641 tgcgcatctg aatgccaact ggctggagat gaataatcgg ctcagcaagg gcgacttgcc
    86701 gtggtatcag gccaatatta ataattatgt taatcagata gacctgttcg tgctggcttt
    86761 acagcactac gccgaacgca aaatgctgct ggtggtggcg atctccctgg ctggcggcat
    86821 cggtattttc acgctggtct tttttacttt gcgccgcata cgccatcagg tggttgcccc
    86881 gctgaatcag ctggttaccg ccagtcagcg tattgaacac gggcagttcg actcgccgcc
    86941 gctggatacc agcctgccga atgagcttgg cctgcttgca aaaaccttta accagatgtc
    87001 gagcgagctg cataaattgt accgttcgct ggaggcgtca gtagaagaaa agacccgcga
    87061 tctccacgag gccaagcgtc gtctggaggt gttgtatcag tgttcgcagg cgctaaacac
    87121 cagccagatt gatgtgcatt gtttccgcca tattttgcag attgttcgcg acaatgaagc
    87181 ggctgaatat ctggagttaa atgtcggtga caactggcgg attagcgaag ggcagccaaa
    87241 cccggaattg ccgatgcaga ttttaccggt gacaatgcaa gagacggttt acggcgaact
    87301 gcactggcaa aatagtcacg tttcatcatc agaaccgctg cttaacagtg tttcgtcgat
    87361 gctgggacgc ggtttgtact ttaatcaggc gcagaagcat tttcagcagt tattgttgat
    87421 ggaagaacgt gcgaccatcg cccgcgaatt gcacgactcg ctggctcagg tactttctta
    87481 cttacgtatc cagttgacgt tactgaagcg ttcgataccg gaagataacg ccaccgcaca
    87541 aagtatcatg gccgatttct cccaggcgct gaatgatgct tatcggcagt tacgcgagct
    87601 gttaactact ttccgcctga cgctgcagca ggcggatctc ccctccgcgt tgagggaaat
    87661 gctggatacg ttacaaaatc aaaccagcgc caaactgacc ctcgactgcc gtctgccaac
    87721 cctggcgctg gatgcgcaaa tgcaggtgca tttgttgcaa attattcgcg aagcggtgct
    87781 gaatgcgatg aagcacgcca acgccagcga aatcgccgtt agctgcgtca ccgcgccgga
    87841 tggcaatcac acagtctata ttcgcgacaa cgggattggc atcggtgaac cgaaagaacc
    87901 cgaaggccat tatggtctga atatcatgcg cgaacgcgcg gaaagattgg gtggaacgct
    87961 gactttttca caaccttccg gcggcggcac gttagtgagt attagctttc gctctgcgga
    88021 gggtgaggaa agtcagctga tgtaatgcct cttattgacc aaagaatagt ggcacttaag
    88081 gttcagtata aaagggcatg ataatttaca ttaactcctt tttttctcca cgattggctc
    88141 gtaccttgcc gctacagtga agcaagtcaa gcctacaacg atacgcagaa acacgaggtc
    88201 ctcttttaat ggcgaatttc tttattgatc gccccatttt tgcctgggtg ctggcaatcc
    88261 tgttgtgtct gacaggtacc ctggcgattt tttcattgcc cgttgaacaa taccccgatc
    88321 tcgcgccacc gaatgtgcga gtgaccgcta actatcccgg cgcatcggcc cagacgctgg
    88381 aaaacaccgt gacccaggtt atcgagcaaa atatgaccgg cctcgataat ctcatgtata
    88441 tgtcgtctca gagcagtggc accggccagg catctgtcac tttaagtttt aaagcaggca
    88501 ccgatccgga cgaagccgtg cagcaagtac aaaaccagct gcaatcagcc atgcgaaagt
    88561 taccgcaggc ggtgcaaaat cagggcgtga cggtgcgtaa aaccggcgat accaatattc
    88621 tgaccattgc cttcgtctct accgatggtt cgatggataa acaggatatt gccgattacg
    88681 ttgccagtaa tattcaggac ccgttaagcc gcgtgaatgg cgtcggggat atcgatgcct
    88741 atggttcgca atattccatg cgtatctggc tggacccggc gaaactcaac agtttccaga
    88801 tgacggctaa agatgtcact gatgccattg agtcacagaa cgcgcagatt gcggttgggc
    88861 aacttggtgg tacgccttcc gtcgataagc aggcgctcaa cgccaccatt aacgcccagt
    88921 cactgctgca aacaccagaa cagttccgcg atatcacctt gcgggtgaat caggacggtt
    88981 cagaggtaag gctgggcgat gtcgccaccg tcgaaatggg ggcggaaaaa tacgattatc
    89041 ttagccgctt caatggtaag ccagcctccg ggctgggggt aaaactggcc tccggcgcga
    89101 acgaaatggc gactgcgcag ctggtgctca atcgtctcga cgagctggcg cagtattttc
    89161 cacacggact ggaatacaag gtggcgtatg aaaccacctc gtttgttaaa gcctccattg
    89221 aagacgtggt gaaaacgctg ctggaagcta tcgctctggt tttcctcgtt atgtatctgt
    89281 tcctgcaaaa cttccgcgcc acgctgatac ccactatcgc cgtgccggtg gtgttgatgg
    89341 gaaccttctc cgtactttat gccttcggtt acagcgtcaa caccttaacc atgttcgcga
    89401 tggtgctggc gatcggtctg ctggtggatg atgccatcgt ggtggtggaa aacgtcgaac
    89461 gtattatgag tgaggaagga ctcactcctc gcgaagccac acgtaaatcg atggggcaga
    89521 tccagggggc actggtcggg attgcgatgg tgctttcggc ggtatttgtg ccaatggcct
    89581 tcttcggcgg caccaccggt gccatctatc gccagttctc tattaccatt gttgcggcga
    89641 tggtgctgtc agtactggta gcgatgatcc tcactccggc tctgtgtgcc acgctactta
    89701 agccactgaa aaaaggtgag catcacgggc aaaaaggctt ttttgcctgg tttaaccaga
    89761 tgtttaaccg caacgccgaa cgctacgaaa aaggggtggc gaaaattctc caccgtagcc
    89821 tgcgctggat tgtgatttat gtcctgctgc ttggcggcat ggtgttcctg ttcctgcgtt
    89881 tgccgacgtc gttcttgcca cttgaagacc gtggcatgtt tactacctcg gtacagttgc
    89941 ccagcggttc aacccaacaa cagaccctga aagtcgttga gcaaatcgag aaatactact
    90001 tcacccatga aaaagacaac atcatgtcgg tgtttgccac tgttggttct ggccctgggg
    90061 gtaacggaca aaacgtggcg agaatgttta tccgcctgaa agactggagc gaacgcgaca
    90121 gtaagaccgg cacctcgttt gccattatcg agcgtgcaac gaaggcgttt aacaagatta
    90181 aagaagctcg cgttatcgcc agcagcccgc cagcaattag cggtcttggt agttctgcag
    90241 gttttgatat ggagttgcag gaccacgctg gagcgggtca cgatgcgctg atggcagcac
    90301 gtaaccagtt gctggcgctg gcggcggaaa acccggagct aacccgtgtg cgccataacg
    90361 gcctcgacga cagtccgcag ttgcagattg atatcgacca gcgtaaagct caggcgctgg
    90421 gcgttgctat cgacgatatt aacgacacac tgcaaaccgc ctggggttcg agctatgtga
    90481 atgactttat ggatcgcggt cgcgtgaaga aagtctatgt gcaggcagct gcgccgtatc
    90541 gcatgctgcc agatgacatc aatctctggt atgtccgaaa taaagatggc ggcatggtgc
    90601 ccttctctgc tttcgcgacc tcacgctggg agacaggctc gccgcgtctg gaacgctata
    90661 acggttattc tgcggtagag attgtagggg aagccgcacc gggggtcagt accggtacgg
    90721 cgatggatat tatggaatcg ttagtgaagc agttgccaaa cggctttggt ctggagtgga
    90781 cggcgatgtc gtatcaggag cggctttccg gcgcgcaggc tccggcgctg tacgccattt
    90841 ccttgctggt ggtattcctg tgtcttgcgg cgttgtatga aagctggtcg gtgccgttct
    90901 cggtaatgct ggtcgtgccg ctgggggtta tcggcgcgct gctggcaacc tggatgcgcg
    90961 ggctggaaaa cgacgtttac ttccaggtgg gcctgttaac ggtcattggt ttatcggcga
    91021 aaaacgccat cctgatcgtc gagtttgcta acgagatgaa ccaaaaaggc cacgacctgt
    91081 ttgaagcgac gctccacgcc tgccgtcagc gtttacgccc gattctgatg acttcgctgg
    91141 catttatctt cggcgtattg ccaatggcaa ccagcacggg tgccggttcc ggtggtcagc
    91201 atgcggtggg tactggcgta atgggcggga tgatttcggc cactattctg gctatttact
    91261 tcgtgccgct gttctttgtg ctggtgcgcc gccgcttccc gctgaagccg cgcccggaat
    91321 aagcaataaa aaaggcgaca tgctaatgtg tcgccttttt caactttccg attaagaacc
    91381 tgctcagcgg gttcttgctg tttgtacttt gtctcaggaa ttacttacga agcataactt
    91441 cgataaagtc tttccagttc cccagttcac gttcaatcat aacaacctct cttataatta
    91501 tgggtattct acggaaacaa tataccgtgg tgaagctaat ttactcgatt gctgcgatga
    91561 ctacctccgg gggacaaacc ttatgtaaat actatggtcc tgcggtgata atttgtatgt
    91621 gatacacagc aacatttcga gatattcata cggcatctaa tactgattta attctg
//
LOCUS       DAZGQL010000005        89255 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.005, whole genome shotgun sequence.
ACCESSION   DAZGQL010000005 DAZGQL010000000
VERSION     DAZGQL010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 89255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 89255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..89255
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.005"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            227..1798
                     /gene="garD"
                     /locus_tag="ACHKIR_000396"
     CDS             227..1798
                     /gene="garD"
                     /locus_tag="ACHKIR_000396"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008867 - galactarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0046392 - galactarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HFO9988190.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPAPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLVGTDDVQGIPLENASIVSLQDEKHVGFQSMVEDILQVAERHLHKLNQRQRET
                     CPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAIP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            1947..2282
                     /gene="prlF"
                     /locus_tag="ACHKIR_000397"
     CDS             1947..2282
                     /gene="prlF"
                     /locus_tag="ACHKIR_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PrlF"
                     /protein_id="HFO9988191.1"
                     /translation="MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHY
                     EILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNNPQKTRPFDIQQGKKLVAGMDVN
                     IDDEIGDDE"
     gene            2282..2746
                     /gene="yhaV"
                     /locus_tag="ACHKIR_000398"
     CDS             2282..2746
                     /gene="yhaV"
                     /locus_tag="ACHKIR_000398"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HFO9988192.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH"
     gene            complement(2801..3610)
                     /gene="agaR"
                     /locus_tag="ACHKIR_000399"
     CDS             complement(2801..3610)
                     /gene="agaR"
                     /locus_tag="ACHKIR_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HFO9988193.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGE"
     gene            3859..5139
                     /gene="kbaZ"
                     /locus_tag="ACHKIR_000400"
     CDS             3859..5139
                     /gene="kbaZ"
                     /locus_tag="ACHKIR_000400"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HFO9988194.1"
                     /translation="MKHLTEMVRQHKAGKTNGIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDGIVAERAARLAKVAEETCL
                     EHFGEADLVYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLSAIWP
                     RIIALVVQPGVEFDHTNVIDYQPVKATALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            5162..5635
                     /gene="agaV"
                     /locus_tag="ACHKIR_000401"
     CDS             5162..5635
                     /gene="agaV"
                     /locus_tag="ACHKIR_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HFO9988195.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            5646..6425
                     /gene="agaW"
                     /locus_tag="ACHKIR_000402"
     CDS             5646..6425
                     /gene="agaW"
                     /locus_tag="ACHKIR_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HFO9988196.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            6415..7293
                     /gene="agaE"
                     /locus_tag="ACHKIR_000403"
     CDS             6415..7293
                     /gene="agaE"
                     /locus_tag="ACHKIR_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HFO9988197.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDQSIGAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRGWSPLRLI
                     AVTVVLGIVGKFCHFL"
     gene            7311..7745
                     /gene="agaF"
                     /locus_tag="ACHKIR_000404"
     CDS             7311..7745
                     /gene="agaF"
                     /locus_tag="ACHKIR_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine/N-acetylgalactosamine
                     transporter subunit IIA"
                     /protein_id="HFO9988198.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGEQSQFIAIDFPETSSTALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKLGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            7742..8875
                     /gene="nagA"
                     /locus_tag="ACHKIR_000405"
     CDS             7742..8875
                     /gene="nagA"
                     /locus_tag="ACHKIR_000405"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFO9988199.1"
                     /translation="MTPVLRARRLLTEEGWLDDHQLRIADGVIAEIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LERIAQRCQRGGPGAQILGSYLEGPYFTPQNKGAHPPELFRELEIAELDQLIAVSQHT
                     LRVVALAPEKEGALQAIRHLKQQNVRVMLGHSAATWQQTRAAFDAGADGLVHCYNGMT
                     GLHHREPGMVGAGLTDKRAWLELIADGHHVHPAAMSLCCCCAKERIVLITDAMQAAGM
                     PDGRYTLCGEEVQMHGGVVRTASGGLAGSTLSVDAAVRNMVELTGVTPAEAIHMASLH
                     PARMLGVDDVLGSLKPGKRASVVALDSGLHAQQIWIQGQLASF"
     gene            9225..10379
                     /gene="agaS"
                     /locus_tag="ACHKIR_000406"
     CDS             9225..10379
                     /gene="agaS"
                     /locus_tag="ACHKIR_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgaS family sugar isomerase"
                     /protein_id="HFO9988200.1"
                     /translation="MPENYTPAAAATGTWTEEEIRHQPRAWIRSLTNIDALRSALNNF
                     LEPLLRKENLQIILTGAGTSAFIGDIIAPWLASHTGKNFSAVPTTDLVTNPMDYLNPA
                     HPLLLISFGRSGNSPESVAAVELANQFVPECYHLPITCNEAGALYQNAINSDNAFALL
                     MPAETHDRGFAMTSSITTMMASCLAVFAPETINSQSFRDVADRCQAILTSLGDFSEGV
                     FGYAPWKRIVYLGSGGLQGAARESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDNET
                     LVVVFVSSHPYTRQYDLDLLAELRRDNQAMRVIAIAAESNDVITAGPHIILPPSRHFI
                     DVEQAFCFLMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVIIHPWQA"
     gene            10392..11252
                     /gene="kbaY"
                     /locus_tag="ACHKIR_000407"
     CDS             10392..11252
                     /gene="kbaY"
                     /locus_tag="ACHKIR_000407"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaY"
                     /protein_id="HFO9988201.1"
                     /translation="MSIISTKYLLQDAQANGYAVPAFNIHNAETIQAILEVCSEMRSP
                     VILAGTPGTFKHIALEEIYALCSAYSTTYNMPLALHLDHHESLDDIRRKVHAGVRSAM
                     IDGSHFPFAENVKLVKSVVDFCHSQDCSVEAELGRLGGVEDDMSVDAESAFLTDPQEA
                     KRFVELTGVDSLAVAIGTAHGLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFV
                     RRTIELGVTKVNVATELKIAFAGAVKAWFAENPQGNDPRYYMRVGMDAMKEVVRNKIN
                     VCGSANRISA"
     gene            11419..11895
                     /gene="agaB"
                     /locus_tag="ACHKIR_000408"
     CDS             11419..11895
                     /gene="agaB"
                     /locus_tag="ACHKIR_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIB"
                     /protein_id="HFO9988202.1"
                     /translation="MSSPNILLTRIDNRLVHGQVGVTWTSTIGANLLVVVDDVVANDD
                     IQQKLMSITAETYGFGIRFFTIEKTINVIGKAAPHQKIFLICRTPQTVRKLVEGGIDL
                     KDVNVGNMHFSEGKKQISSKVYVDDQDLTDLRFIKQRGVNVFIQDVPGDQKEQIPD"
     gene            11934..12737
                     /gene="agaC"
                     /locus_tag="ACHKIR_000409"
     CDS             11934..12737
                     /gene="agaC"
                     /locus_tag="ACHKIR_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIC"
                     /protein_id="HFO9988203.1"
                     /translation="MHEITLLQGLSLAALVFVLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIQVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            12727..13518
                     /gene="agaD"
                     /locus_tag="ACHKIR_000410"
     CDS             12727..13518
                     /gene="agaD"
                     /locus_tag="ACHKIR_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IID"
                     /protein_id="HFO9988204.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPLLKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHIGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            13519..14286
                     /gene="agaI"
                     /locus_tag="ACHKIR_000411"
     CDS             13519..14286
                     /gene="agaI"
                     /locus_tag="ACHKIR_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HFO9988205.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQQVDVSQLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQPLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTAGRPVTRGITLGLKDILNAREVLLLVTGEGK
                     QDATERFLTAKVSTAIPASFLWLHNYFTCMIDEMCRR"
     gene            complement(14336..15199)
                     /gene="rsmI"
                     /locus_tag="ACHKIR_000412"
     CDS             complement(14336..15199)
                     /gene="rsmI"
                     /locus_tag="ACHKIR_000412"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000809248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFO9988206.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQGE"
     gene            15264..17300
                     /gene="lpoA"
                     /locus_tag="ACHKIR_000413"
     CDS             15264..17300
                     /gene="lpoA"
                     /locus_tag="ACHKIR_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HFO9988207.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAAEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTVDGVASPAQASVSELTGEQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            17258..17653
                     /gene="yraN"
                     /locus_tag="ACHKIR_000414"
     CDS             17258..17653
                     /gene="yraN"
                     /locus_tag="ACHKIR_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HFO9988208.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDVWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGRITVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            17673..18263
                     /gene="diaA"
                     /locus_tag="ACHKIR_000415"
     CDS             17673..18263
                     /gene="diaA"
                     /locus_tag="ACHKIR_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001483830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HFO9988209.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDV"
     gene            18273..18848
                     /gene="dolP"
                     /locus_tag="ACHKIR_000416"
     CDS             18273..18848
                     /gene="dolP"
                     /locus_tag="ACHKIR_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HFO9988210.1"
                     /translation="MKALSPIAVLLSALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKEARINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(18961..20001)
                     /gene="yraQ"
                     /locus_tag="ACHKIR_000417"
     CDS             complement(18961..20001)
                     /gene="yraQ"
                     /locus_tag="ACHKIR_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFO9988211.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVLLGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPLLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQGGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLV"
     gene            complement(20074..20709)
                     /gene="yraR"
                     /locus_tag="ACHKIR_000418"
     CDS             complement(20074..20709)
                     /gene="yraR"
                     /locus_tag="ACHKIR_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HFO9988212.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLTDALAQVTDPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            20837..21355
                     /gene="yhbO"
                     /locus_tag="ACHKIR_000419"
     CDS             20837..21355
                     /gene="yhbO"
                     /locus_tag="ACHKIR_000419"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HFO9988213.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVAIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(21335..21778)
                     /gene="yhbP"
                     /locus_tag="ACHKIR_000420"
     CDS             complement(21335..21778)
                     /gene="yhbP"
                     /locus_tag="ACHKIR_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HFO9988214.1"
                     /translation="METLTAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARQAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRNSGTEQA"
     gene            21829..22131
                     /gene="yhbQ"
                     /locus_tag="ACHKIR_000421"
     CDS             21829..22131
                     /gene="yhbQ"
                     /locus_tag="ACHKIR_000421"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HFO9988215.1"
                     /translation="MTPWFLYLIRTADDKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPKIKSD"
     gene            complement(22118..22621)
                     /gene="yhbS"
                     /locus_tag="ACHKIR_000422"
     CDS             complement(22118..22621)
                     /gene="yhbS"
                     /locus_tag="ACHKIR_000422"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO9988216.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(22615..23139)
                     /gene="ubiT"
                     /locus_tag="ACHKIR_000423"
     CDS             complement(22615..23139)
                     /gene="ubiT"
                     /locus_tag="ACHKIR_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HFO9988217.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            23348..24343
                     /gene="ubiU"
                     /locus_tag="ACHKIR_000424"
     CDS             23348..24343
                     /gene="ubiU"
                     /locus_tag="ACHKIR_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFO9988218.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            24352..25230
                     /gene="ubiV"
                     /locus_tag="ACHKIR_000425"
     CDS             24352..25230
                     /gene="ubiV"
                     /locus_tag="ACHKIR_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFO9988219.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAAASSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAILDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            25311..26318
                     /gene="yhbW"
                     /locus_tag="ACHKIR_000426"
     CDS             25311..26318
                     /gene="yhbW"
                     /locus_tag="ACHKIR_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase-like monooxygenase"
                     /protein_id="HFO9988220.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(26436..27680)
                     /gene="mtr"
                     /locus_tag="ACHKIR_000427"
     CDS             complement(26436..27680)
                     /gene="mtr"
                     /locus_tag="ACHKIR_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HFO9988221.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPTTLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAMGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIMLIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(27834..29723)
                     /gene="deaD"
                     /locus_tag="ACHKIR_000428"
     CDS             complement(27834..29723)
                     /gene="deaD"
                     /locus_tag="ACHKIR_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HFO9988222.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFSGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(29716..29796)
                     /gene="yrbN"
                     /locus_tag="ACHKIR_000429"
     CDS             complement(29716..29796)
                     /gene="yrbN"
                     /locus_tag="ACHKIR_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HFO9988223.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(29903..30787)
                     /gene="nlpI"
                     /locus_tag="ACHKIR_000430"
     CDS             complement(29903..30787)
                     /gene="nlpI"
                     /locus_tag="ACHKIR_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HFO9988224.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(30896..33031)
                     /gene="pnp"
                     /locus_tag="ACHKIR_000431"
     CDS             complement(30896..33031)
                     /gene="pnp"
                     /locus_tag="ACHKIR_000431"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFO9988225.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YNGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(33278..33547)
                     /gene="rpsO"
                     /locus_tag="ACHKIR_000432"
     CDS             complement(33278..33547)
                     /gene="rpsO"
                     /locus_tag="ACHKIR_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFO9988226.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(33696..34640)
                     /gene="truB"
                     /locus_tag="ACHKIR_000433"
     CDS             complement(33696..34640)
                     /gene="truB"
                     /locus_tag="ACHKIR_000433"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFO9988227.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(34640..35041)
                     /gene="rbfA"
                     /locus_tag="ACHKIR_000434"
     CDS             complement(34640..35041)
                     /gene="rbfA"
                     /locus_tag="ACHKIR_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFO9988228.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(35205..37877)
                     /gene="infB"
                     /locus_tag="ACHKIR_000435"
     CDS             complement(35205..37877)
                     /gene="infB"
                     /locus_tag="ACHKIR_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFO9988229.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(37902..39389)
                     /gene="nusA"
                     /locus_tag="ACHKIR_000436"
     CDS             complement(37902..39389)
                     /gene="nusA"
                     /locus_tag="ACHKIR_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFO9988230.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGIDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(39417..39869)
                     /gene="rimP"
                     /locus_tag="ACHKIR_000437"
     CDS             complement(39417..39869)
                     /gene="rimP"
                     /locus_tag="ACHKIR_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFO9988231.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(40076..40152)
                     /locus_tag="ACHKIR_000438"
     tRNA            complement(40076..40152)
                     /locus_tag="ACHKIR_000438"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40116..40118),aa:Met,seq:cat)
     gene            40500..41843
                     /gene="argG"
                     /locus_tag="ACHKIR_000439"
     CDS             40500..41843
                     /gene="argG"
                     /locus_tag="ACHKIR_000439"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HFO9988232.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSAASGVPQVENLENKGQ"
     gene            complement(41851..43476)
                     /gene="yhbX"
                     /locus_tag="ACHKIR_000440"
     CDS             complement(41851..43476)
                     /gene="yhbX"
                     /locus_tag="ACHKIR_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO9988233.1"
                     /translation="MTVFNKFVRSFKSHWLLYLCVIVFGITNLVASSGAHMVQRLLFF
                     VLTILVVKRISSLPLRLLVAAPFVLLTAADMSISLYSWCTFGTTFNDGFAISVLQSDP
                     DEVVKMLGMYIPYLCAFAFLSLLFLAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQ
                     FAYKDAKNKKAFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVR
                     DTGIDTYVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQAISGAPYTALSVP
                     LSLTADSVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETV
                     YVRGFDELLLPHLSQALQQNTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDAC
                     YDNSIHYTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHV
                     PMFIWYSPVLGDGVDRTTENNIFSTAYNNYLINAWMGVTKPEQPKTLEEVIAHYKGDS
                     RVVDANHDVFDYVMLRKEFTEDKQGNPTPEGQG"
     gene            complement(43933..44019)
                     /locus_tag="ACHKIR_000441"
     tRNA            complement(43933..44019)
                     /locus_tag="ACHKIR_000441"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(43983..43985),aa:Leu,seq:gag)
     gene            complement(44034..44366)
                     /gene="secG"
                     /locus_tag="ACHKIR_000442"
     CDS             complement(44034..44366)
                     /gene="secG"
                     /locus_tag="ACHKIR_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFO9988234.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQIQPAAPA
                     KPTSDIPN"
     gene            complement(44594..45931)
                     /gene="glmM"
                     /locus_tag="ACHKIR_000443"
     CDS             complement(44594..45931)
                     /gene="glmM"
                     /locus_tag="ACHKIR_000443"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFO9988235.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGSRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(45924..46772)
                     /gene="folP"
                     /locus_tag="ACHKIR_000444"
     CDS             complement(45924..46772)
                     /gene="folP"
                     /locus_tag="ACHKIR_000444"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFO9988236.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYHDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(46862..48796)
                     /gene="ftsH"
                     /locus_tag="ACHKIR_000445"
     CDS             complement(46862..48796)
                     /gene="ftsH"
                     /locus_tag="ACHKIR_000445"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFO9988237.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(48896..49525)
                     /gene="rlmE"
                     /locus_tag="ACHKIR_000446"
     CDS             complement(48896..49525)
                     /gene="rlmE"
                     /locus_tag="ACHKIR_000446"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HFO9988238.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            49651..49944
                     /gene="yhbY"
                     /locus_tag="ACHKIR_000447"
     CDS             49651..49944
                     /gene="yhbY"
                     /locus_tag="ACHKIR_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFO9988239.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(50100..50576)
                     /gene="greA"
                     /locus_tag="ACHKIR_000448"
     CDS             complement(50100..50576)
                     /gene="greA"
                     /locus_tag="ACHKIR_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFO9988240.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            50824..52257
                     /gene="dacB"
                     /locus_tag="ACHKIR_000449"
     CDS             50824..52257
                     /gene="dacB"
                     /locus_tag="ACHKIR_000449"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFO9988241.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYITQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLRRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(52298..53470)
                     /gene="cgtA"
                     /locus_tag="ACHKIR_000450"
     CDS             complement(52298..53470)
                     /gene="cgtA"
                     /locus_tag="ACHKIR_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HFO9988242.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(53486..54451)
                     /gene="yhbE"
                     /locus_tag="ACHKIR_000451"
     CDS             complement(53486..54451)
                     /gene="yhbE"
                     /locus_tag="ACHKIR_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO9988243.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLGPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(54578..54835)
                     /gene="rpmA"
                     /locus_tag="ACHKIR_000452"
     CDS             complement(54578..54835)
                     /gene="rpmA"
                     /locus_tag="ACHKIR_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFO9988244.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(54856..55167)
                     /gene="rplU"
                     /locus_tag="ACHKIR_000453"
     CDS             complement(54856..55167)
                     /gene="rplU"
                     /locus_tag="ACHKIR_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFO9988245.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            55426..56397
                     /gene="ispB"
                     /locus_tag="ACHKIR_000454"
     CDS             55426..56397
                     /gene="ispB"
                     /locus_tag="ACHKIR_000454"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HFO9988246.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            56625..56903
                     /gene="sfsB"
                     /locus_tag="ACHKIR_000455"
     CDS             56625..56903
                     /gene="sfsB"
                     /locus_tag="ACHKIR_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HFO9988247.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWIIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(56951..58210)
                     /gene="murA"
                     /locus_tag="ACHKIR_000456"
     CDS             complement(56951..58210)
                     /gene="murA"
                     /locus_tag="ACHKIR_000456"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFO9988248.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(58265..58519)
                     /gene="ibaG"
                     /locus_tag="ACHKIR_000457"
     CDS             complement(58265..58519)
                     /gene="ibaG"
                     /locus_tag="ACHKIR_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HFO9988249.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(58679..58972)
                     /gene="mlaB"
                     /locus_tag="ACHKIR_000458"
     CDS             complement(58679..58972)
                     /gene="mlaB"
                     /locus_tag="ACHKIR_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HFO9988250.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(58972..59607)
                     /gene="mlaC"
                     /locus_tag="ACHKIR_000459"
     CDS             complement(58972..59607)
                     /gene="mlaC"
                     /locus_tag="ACHKIR_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HFO9988251.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQRDAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(59626..60177)
                     /gene="mlaD"
                     /locus_tag="ACHKIR_000460"
     CDS             complement(59626..60177)
                     /gene="mlaD"
                     /locus_tag="ACHKIR_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HFO9988252.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(60182..60964)
                     /gene="mlaE"
                     /locus_tag="ACHKIR_000461"
     CDS             complement(60182..60964)
                     /gene="mlaE"
                     /locus_tag="ACHKIR_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HFO9988253.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(60972..61781)
                     /gene="mlaF"
                     /locus_tag="ACHKIR_000462"
     CDS             complement(60972..61781)
                     /gene="mlaF"
                     /locus_tag="ACHKIR_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HFO9988254.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            61991..62968
                     /gene="yrbG"
                     /locus_tag="ACHKIR_000463"
     CDS             61991..62968
                     /gene="yrbG"
                     /locus_tag="ACHKIR_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HFO9988255.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            62982..63968
                     /gene="kdsD"
                     /locus_tag="ACHKIR_000464"
     CDS             62982..63968
                     /gene="kdsD"
                     /locus_tag="ACHKIR_000464"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HFO9988256.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGVTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            63989..64555
                     /gene="kdsC"
                     /locus_tag="ACHKIR_000465"
     CDS             63989..64555
                     /gene="kdsC"
                     /locus_tag="ACHKIR_000465"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HFO9988257.1"
                     /translation="MSKAGASLATCYGPVSADVIAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSIAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            64552..65127
                     /gene="lptC"
                     /locus_tag="ACHKIR_000466"
     CDS             64552..65127
                     /gene="lptC"
                     /locus_tag="ACHKIR_000466"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HFO9988258.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            65096..65653
                     /gene="lptA"
                     /locus_tag="ACHKIR_000467"
     CDS             65096..65653
                     /gene="lptA"
                     /locus_tag="ACHKIR_000467"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HFO9988259.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            65660..66385
                     /gene="lptB"
                     /locus_tag="ACHKIR_000468"
     CDS             65660..66385
                     /gene="lptB"
                     /locus_tag="ACHKIR_000468"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HFO9988260.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            66433..67866
                     /gene="rpoN"
                     /locus_tag="ACHKIR_000469"
     CDS             66433..67866
                     /gene="rpoN"
                     /locus_tag="ACHKIR_000469"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFO9988261.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            67889..68176
                     /gene="hpf"
                     /locus_tag="ACHKIR_000470"
     CDS             67889..68176
                     /gene="hpf"
                     /locus_tag="ACHKIR_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HFO9988262.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            68294..68785
                     /gene="ptsN"
                     /locus_tag="ACHKIR_000471"
     CDS             68294..68785
                     /gene="ptsN"
                     /locus_tag="ACHKIR_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HFO9988263.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            68831..69685
                     /gene="rapZ"
                     /locus_tag="ACHKIR_000472"
     CDS             68831..69685
                     /gene="rapZ"
                     /locus_tag="ACHKIR_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFO9988264.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            69682..69954
                     /gene="npr"
                     /locus_tag="ACHKIR_000473"
     CDS             69682..69954
                     /gene="npr"
                     /locus_tag="ACHKIR_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HFO9988265.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            70167..70799
                     /gene="yrbL"
                     /locus_tag="ACHKIR_000474"
     CDS             70167..70799
                     /gene="yrbL"
                     /locus_tag="ACHKIR_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HFO9988266.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(70796..71524)
                     /gene="mtgA"
                     /locus_tag="ACHKIR_000475"
     CDS             complement(70796..71524)
                     /gene="mtgA"
                     /locus_tag="ACHKIR_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HFO9988267.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSAPSGYVRS
                     RQAWILRQMYQLGGESFMQQHQLD"
     gene            complement(71521..72174)
                     /gene="elbB"
                     /locus_tag="ACHKIR_000476"
     CDS             complement(71521..72174)
                     /gene="elbB"
                     /locus_tag="ACHKIR_000476"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HFO9988268.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFASLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(72404..74740)
                     /gene="arcB"
                     /locus_tag="ACHKIR_000477"
     CDS             complement(72404..74740)
                     /gene="arcB"
                     /locus_tag="ACHKIR_000477"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HFO9988269.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLALVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(74836..75765)
                     /gene="yhcC"
                     /locus_tag="ACHKIR_000478"
     CDS             complement(74836..75765)
                     /gene="yhcC"
                     /locus_tag="ACHKIR_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562163.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFO9988270.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQTECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTA"
     gene            76440..80900
                     /gene="gltB"
                     /locus_tag="ACHKIR_000479"
     CDS             76440..80900
                     /gene="gltB"
                     /locus_tag="ACHKIR_000479"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HFO9988271.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEDHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVEQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHAGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            80913..82331
                     /gene="gltD"
                     /locus_tag="ACHKIR_000480"
     CDS             80913..82331
                     /gene="gltD"
                     /locus_tag="ACHKIR_000480"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HFO9988272.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDLSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEDNDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            82514..83641
                     /gene="yhcG"
                     /locus_tag="ACHKIR_000481"
     CDS             82514..83641
                     /gene="yhcG"
                     /locus_tag="ACHKIR_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HFO9988273.1"
                     /translation="MESLSERTSTGYQQIHDGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIKRLSKDLSLRYKRGFSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKSFPLPWSTYVRLLSVKNPDARSFYE
                     KETLRCGWSVRQLERQITTQFYERTLLSHDKSAMLQQHAPAEMHILPQQAIRDPFVLE
                     FLELKDEYSESDFEEALINHLMDFMLELGDDFAFVGRQRRLRIDDNWFRVDLLFFHRR
                     LRCLLIVDLKVGKFSYSDAGQMNMYLNYAKEHWTLPDENPPIGLVLCAEKGAGEAHYA
                     LAGLPNTVLASEYKMQLPDEKRLADELVRTQAVLEEGYRRR"
     gene            complement(83701..84165)
                     /gene="nanQ"
                     /locus_tag="ACHKIR_000482"
     CDS             complement(83701..84165)
                     /gene="nanQ"
                     /locus_tag="ACHKIR_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate anomerase"
                     /protein_id="HFO9988274.1"
                     /translation="MIMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQCDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGTAGTARQCEEFHHEDDYQ
                     LCSAIENEQAIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(84162..85037)
                     /gene="nanK"
                     /locus_tag="ACHKIR_000483"
     CDS             complement(84162..85037)
                     /gene="nanK"
                     /locus_tag="ACHKIR_000483"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HFO9988275.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALHDA
                     LSALVSPLQVHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTDLPTIA
                     INDAQAAAWAEYQALEGDITEMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHCSA
                     HVLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(85034..85723)
                     /gene="nanE"
                     /locus_tag="ACHKIR_000484"
     CDS             complement(85034..85723)
                     /gene="nanE"
                     /locus_tag="ACHKIR_000484"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFO9988276.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSDAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(85771..87261)
                     /gene="nanT"
                     /locus_tag="ACHKIR_000485"
     CDS             complement(85771..87261)
                     /gene="nanT"
                     /locus_tag="ACHKIR_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HFO9988277.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHGGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYDPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGRAKNDLVKTKS"
     gene            complement(87371..88264)
                     /gene="nanA"
                     /locus_tag="ACHKIR_000486"
     CDS             complement(87371..88264)
                     /gene="nanA"
                     /locus_tag="ACHKIR_000486"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HFO9988278.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(88386..89177)
                     /gene="nanR"
                     /locus_tag="ACHKIR_000487"
     CDS             complement(88386..89177)
                     /gene="nanR"
                     /locus_tag="ACHKIR_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312126.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HFO9988279.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVADQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT        21705 a        24053 c        22475 g        21022 t
ORIGIN      
        1 agtactattt aaatattatt ttgagcatat gcacataagg ttgcgcgcta aagcacagat
       61 ttgcgcttta ccttgccggg cggcactgca atccctgaaa tgattgacat tgatcacatt
      121 tctgcattta aactcctgac attcttattt cacccaatga agttatttat ttttaaatga
      181 gaccaggtcc tcattttaat aacccctggc tggagaatat tgcacaatgg ccaacatcga
      241 aatcagacaa gaaacgccaa ctgcgtttta tataaaagtt cacgacacag ataatgtggc
      301 aattattgtt aatgataatg gcctgaaagc aggaacgcgt tttccggatg ggctggaatt
      361 aattgaacat attccccagg ggcataaagt cgcattgctg gacattccgg ctaatggtga
      421 aattattcgt tatggcgaag tgattggtta cgccgtgcgc gcaatcccac gcggaagctg
      481 gatcgacgaa tcaatggttg tactaccgga agcgccgccg ttacacacgc tgccactggc
      541 aaccaaagtc ccggcaccct taccgccact ggaaggatac acctttgagg gctatcgcaa
      601 tgccgatggc agcgtgggca ccaaaaacct gctcggcatc accaccagcg tccactgtgt
      661 ggcaggcgtg gtggactatg tagtaaaaat cattgaacgc gatctgctgc cgaaataccc
      721 gaacgtcgat ggcgtggtgg ggctgaatca tctgtacggt tgtggcgtgg cgattaacgc
      781 tccggcggcg gtggtgccca ttcggaccat tcacaacatt tcgcttaacc caaactttgg
      841 cggcgaggtg atggtgattg gcctgggttg cgagaaatta cagccagaac gcctgctggt
      901 cggcacggat gatgtacaag gtattccgct ggagaacgcc agtattgtca gcttgcagga
      961 tgaaaagcat gtcggttttc agtccatggt cgaggatatt ttgcaggtcg ccgaacgcca
     1021 tttgcacaaa ctgaatcaac ggcagcgaga aacctgtcca gcttcagaac tggttgtcgg
     1081 catgcagtgc ggtggcagcg atgcgttttc tggcgtaacg gcaaacccgg cggttgggta
     1141 tgcgtctgat ctactggtgc gctgcggcgc aacggtgatg ttttcagaag tcacggaagt
     1201 gcgtgacgcg atccatctgc tgacaccacg cgcagtgaac gaagaggtcg gcaaacggct
     1261 gctggaggag atggagtggt acgataacta tctcaatatg ggaaaaaccg accgtagcgc
     1321 caacccttcg ccgggcaaca agaaaggcgg tctggcaaat gtggtggaga aagcgctcgg
     1381 ctccattgct aaatcgggta aaagcgcaat tgttgaagtg ctgtcgcccg gtcaacgccc
     1441 gactaaacgc ggattaattt acgccgcgac gccagccagc gattttgtct gtggcacaca
     1501 acaggtggct tcagggatta ccgtgcaagt gtttacgacc ggtcgcggta cgccgtacgg
     1561 cctgatggcg atacccgtca ttaaaatggc gacccgcacc gagctggcga accgctggtt
     1621 tgatttaatg gatattaacg cgggcactat cgctaccggc gaagaaacca ttgaagaagt
     1681 gggctggaag ttgttccact ttattctcga cgtcgccagc gggaagaaga aaactttctc
     1741 ggatcaatgg ggactgcata accagctggc ggtgtttaac ccggcaccag tgacctgatt
     1801 tcttttggaa cgtccctcgc aaaacatggc cttagtgcca tgtttttatt gtttaaagtc
     1861 cacacatcca ttaataatgc atttgcatta ccttagttca agcttataat ttgagcagaa
     1921 aacaggctgt aaaaggacag taaataatgc ccgctaatgc tcgctctaac gctgtactga
     1981 ccactgaatc aaaggtcact atacgcggac aaacaactat ccccgcgcca gtgcgtgagg
     2041 ccttaaaact gaagccaggc ctggacagca ttcattacga aattctgcct ggtgggcaag
     2101 tatttatgtg ccgactggga gatgaacagg aggatcatac tatgaatgca tttttgcgtt
     2161 ttctggatgc agatatccag aacaacccgc aaaaaactcg tccattcgac attcaacaag
     2221 gaaagaaact tgtcgctggc atggacgtca acattgacga tgagattggc gacgacgaat
     2281 aatggatttt ccacaaaggg ttaatggttg ggcgctatat gctcatccct gttttcagga
     2341 aacctacgat gctttagttg ccgaagtcga ggcattaaag ggaaaagatc ctgaaaatta
     2401 tcagagaaaa gctgccacaa agttattggc ggtggtccat aaagtgattg aggagcatat
     2461 cacggtcaat ccatcatcac cggcattccg tcatggcaag tcgttaggct ctgggaaaaa
     2521 taaagactgg tcacgggtaa aatttggtgc tggtcgctat cgtctcttct ttcgttacag
     2581 tgaaaaagag aaagtcatca ttctgggatg gatgaacgat gaaaacacgc tgcgcaccta
     2641 cggtaaaaaa acagatgcct ataccgtatt cagtaaaatg ttaaaaagag gacatcctcc
     2701 tgccgactgg gaaagcctca cccaagagac agaagaaaac cattgatggt gttcacaatg
     2761 cccgccgaac gcattggtgt tgaccagcgg gcatcctttc tcattccccg accagaatca
     2821 cttcaacccc agcctttctc agtccttcca gactatccgc aggaatgcct tcatcaacaa
     2881 tgatcatgtc gatacgttga gtatcaatga tcttatgtaa actagaacga ttgaacttac
     2941 tggaatcggt gaccacgatg atccgttccg caacttcgca catccgacgg tttaaacggg
     3001 cttcatcttc attatgcgtg ctgacgccgc gctccagatc gatcgcatct acaccaagaa
     3061 acagcatatc gaagtggtaa ttttgcagcg attgctcagc ctgatcaccg taaaaagatt
     3121 gcgactgacg gcgcaaatgc cccccggtca tcagcagctc aacgccttcc gcttccagca
     3181 acgcattagc cacgttcata ccgttggtca tcgcaattac gtcggtgtgc ttgcgcatca
     3241 gacgagcaat ctcaaaagtg gtggtcccgg agtcgaggat cacccgatga ccaggctgaa
     3301 tcaactcaac ggcagctttc gcaacgctgc gtttcatcgc ggtgttcagc gcgcttttat
     3361 cttccactga tggctcgact gacggcgttg tgctatcgca gatcaacgcg ccaccatagg
     3421 cacgcacagc gatcccctgc ttttccagaa acgccagatc gttgcggatc gtcacagtag
     3481 atacaccata caacgccgat agatcattaa cttgcacact tccttgctgg cgcaggcgtt
     3541 gaatgatctg ctctcgtcgc tcgctggtgc ctgtcactcg cttctcacct gaagcgtcgg
     3601 tattactcat agtaagtcct ttcgtaaaac tttcgtttca tttcgttttg cctattaacg
     3661 cctttctatt aagcaaatgc aagcccacct tacccattgg cgcaagctac tctcgtttca
     3721 gtggctttca ttatgtttct tttgtgaatc agatcagaaa accattatct ttcgttttat
     3781 ttttatctca ccatgacgca gtatcaactg aaacaaaacg aaagattaat gtcgcagtaa
     3841 tctgaactgg agaggaaagt gaaacatctg acagaaatgg tgagacagca caaagcgggc
     3901 aaaacaaatg ggatttatgc cgtttgttcc gcacatccgc tggtgctgga agctgcaatc
     3961 cgctacgcca gtgcaaacca aacgccgtta ctgattgaag caacctccaa tcaggtggac
     4021 cagttcggcg gttataccgg aatgacgccc gccgattttc gcggctttgt ttgtcagctc
     4081 gccgactcgt tgaatttccc gcaggatgcg ctgattctgg gtggtgacca tctggggcca
     4141 aaccgctggc aaaacctgcc tgccgctcag gcaatggcca atgccgatga tttgattaaa
     4201 agctacgtgg cggcaggatt caaaaaaatt caccttgatt gcagcatgtc ctgtcaggac
     4261 gatccgattc ccttaaccga tggcatcgtg gccgaacgcg ccgcccgtct ggcgaaagtg
     4321 gcggaagaaa cctgtcttga acactttggc gaagccgatc tggtgtatgt cattggcacc
     4381 gaagtaccgg tacctggcgg cgcgcatgaa accttaagcg agctggcggt caccacgccg
     4441 gatgccgccc gcgccacgct ggaagcccat cgtcacgcct ttgaaaagca aggtctgagt
     4501 gccatctggc cacgcatcat tgctctggtg gtacaacccg gcgtcgaatt cgatcacacc
     4561 aacgttattg attatcagcc cgtcaaagcg accgccttaa gccagatggt cgaaaactac
     4621 gaaacgctga ttttcgaagc gcactctacc gattaccaaa cgccgcaatc gttgcgccag
     4681 ctagtgattg accactttgc catcctgaaa gttggcccgg cgctgacctt cgccctgcgt
     4741 gaagctctgt tctctctggc ggcgattgaa gaagaactgg taccagcaaa agcctgttct
     4801 ggtctgcgtc aggtgctgga aaacgtgatg ctcgaccgcc cggaatactg gcaaagccac
     4861 taccacggtg acggcaacgc gcgtcgtctg gcgcgtggtt atagctactc ggatcgcgtg
     4921 cgctattact ggccggacag ccagattgat gacgctttcg ctcatctggt tcgtaatctg
     4981 gcggattcac caattccgct gccgctgatc agtcagtatc tgccgctgca gtacgtgaaa
     5041 gttcgctccg gcgagctgca gccaacgcca cgggaactca ttatcaacca tattcaggac
     5101 atcctggcgc agtaccacac agcctgtgaa ggccaataag caaaacaaag aggaacacgc
     5161 tatgccaaat attgttttaa gtcggattga tgaacgcttg attcacggtc aggtcggcgt
     5221 tcagtgggtc ggatttgcgg gggcaaatct ggtgctggta gccaacgatg aggttgccga
     5281 agatccggta caacaaaacc tgatggaaat ggtactggca gaagggatcg ccgtacgttt
     5341 ctggacgctg caaaaagtca tcgacaacat tcatcgcgcc gccgatcgac agaaaatcct
     5401 gctagtttgt aaaacaccgg ccgatttcct gacgttggtg aaaggtggcg ttccggtgaa
     5461 tcgcattaac gttggcaata tgcactacgc caatggcaag caacaaatcg ccaaaacggt
     5521 ttctgtggat gcgggtgata tcgcagcatt taacgatctg aaagccgctg gggtggaatg
     5581 cttcgttcag ggcgtcccga cagagcctgc tgtagacctc tttaaattac tttgagggat
     5641 tcatcatgga aatcagtctg ttgcaggcat ttgcgttggg aattatcgcc tttatcgctg
     5701 gcctggatat gtttaacggc ttaacccata tgcaccgccc ggtggttctc ggcccgttgg
     5761 tcgggctggt acttggcgat ctgcataccg gtattttaac cggcggtacg ctggaactgg
     5821 tgtggatggg gctggcccct ctggcgggcg cacagccgcc taacgtgatt atcggtacta
     5881 tcgtcggcac ggcgtttgcc attactactg gcgtgaaacc cgatgtcgcg gtcggtgtcg
     5941 ccgtaccttt cgctgtcgca gtacagatgg ggattacctt cctgttctcg gtgatgtccg
     6001 gcgtgatgtc ccgatgcgac cggatggcgg agaacgccga tactcgcggc attgaacgcg
     6061 tgaactatct ggcattgctg gcactcggca cgttctattt tctctgcgct ttcctgccta
     6121 tctacttcgg cgcggaacat gcaaaaacca tcattgatgt cctgccgcaa cgattaattg
     6181 atggcctcgg cgtcgcgggc ggcatcatgc cagcaatcgg ttttgccgtg ctgctgaaaa
     6241 tcatgatgaa aaacgtctac atcccctact tcatcctggg ctttgttgcc gcggcctggc
     6301 tcaagttacc ggtgctggct attgctgccg ctgcgctggc gatggcgctg atcgacctgc
     6361 tgcgtaaatc tcctgaaccg actcaacctg cggcacagaa agaggaattt gaagatggca
     6421 tctaatcaaa ccaccctgcc gaacgtcagc gaaaatgaag aaacactgct gactggcgtc
     6481 aatgaaaacg tgtatgaaga tcagagcatt ggcgcggagc tgacgaaaaa agatatcaac
     6541 cgcgtcgcct ggcgttccat gctgttacag gcttctttca actacgaacg tatgcaggct
     6601 tccggctggc tgtacggcct gctgccagca ctgaaaaaga tccacactaa taaacgcgac
     6661 ctggcgcgcg ccatgaaggg gcatatgggt ttcttcaata cccatccgtt tctggtgacg
     6721 tttgttatcg gcattatcct tgcgatggag cgttctaagc aggacgttaa cagtattcag
     6781 agcaccaaaa ttgccgtcgg tgcgccgctc ggcgggattg gcgatgcgat gttctggcta
     6841 acgctactgc cgatttgtgg cgggataggt gcaagcctcg cgctacaagg ctctattctt
     6901 ggcgctgtcg tctttattgt gctgttcaac gtggtgcacc tggggctgcg ttttggtctg
     6961 gcgcattatg cttaccgcat gggcgtggcg gcgattccac tcattaaagc aaataccaaa
     7021 aaagtcggcc atgcggcatc tatcgttggg atgacggtaa tcggcgcgct ggtggcaacc
     7081 tatgttcgtt taagcaccac gctggaaatc accgcgggcg acgcagtggt taagttacag
     7141 gctgatgtta tcgacaaact gatgccagcc ttcttaccgc tggtctacac cctgaccatg
     7201 ttctggctgg tacgccgcgg ctggagtccg ctgcgcctga ttgcagtgac cgtggttctc
     7261 ggcatcgtcg gtaaattctg ccatttcctt taaatacaat gaggtattag atgttaagta
     7321 ttattttgac agggcatggc gggtttgcca gtggcatgga aaaagcaatg aagcaaatcc
     7381 tcggcgagca atcgcagttt attgccatcg attttccgga aacctcaagc accgcgctgc
     7441 ttacatcgca gcttgaagag gccatcgccc aactggattg tgaagacggc attgtttttc
     7501 tgacggattt gctgggcggc acgccgtttc gcgtagcttc aacgctggcg atgcaaaaac
     7561 tgggctgtga agtgattacc ggcaccaatt tgcaactgct actggagatg gtgctggagc
     7621 gcgaagggtt aagcggtgaa gagtttcgtg tgcaggcgct ggagtgtgga catcgtgggc
     7681 tgaccagtct ggtggacgag ttaggccgct gccatgagga atgtccggtc gaggaaggaa
     7741 tatgacaccc gttttgcgcg ccagaaggct gctgactgaa gagggatggc tcgatgacca
     7801 tcagttgcgt attgctgacg gtgtaatcgc agaaatcgaa ccgattccag tgggcgtgac
     7861 tgaacgcgat gcggaactgc tctgccccgc ttacatcgac acccatgtac acggtggtgc
     7921 gggcgttgat gtaatggatg acgcgccgga tgtgctcgac aagctggcaa tgcacaaagc
     7981 acgcgaaggt gtcggcagtt ggttaccgac caccgtaacc gcgccgctta ataccattca
     8041 tgcggcgctg gaacgtattg ctcaacgttg ccaacgcggc ggacctggtg cacaaatact
     8101 ggggagttat ctcgaaggac cgtacttcac gccgcagaat aaaggcgcgc atccgccgga
     8161 gctgtttcgc gagctggaaa ttgccgagct ggatcaattg attgccgttt ctcagcacac
     8221 cttgcgcgtg gtggcgctgg caccggaaaa agaaggggca ttgcaggcca tccgccatct
     8281 taaacagcaa aacgtaaggg tgatgctggg gcatagcgcg gcgacctggc aacaaacccg
     8341 cgccgcgttt gatgctggtg ccgacggcct ggtgcattgc tataacggga tgacaggttt
     8401 acatcaccgc gaaccgggaa tggttggcgc gggattaacg gacaagcgcg cctggctgga
     8461 actgatagcc gatggtcatc atgtgcatcc ggcggcgatg tcgctgtgtt gttgctgtgc
     8521 gaaagagaga atcgtgctca tcaccgacgc gatgcaggca gccgggatgc cggatggtcg
     8581 ctatacgtta tgtggcgaag aagtgcagat gcacggtggc gttgtccgta ccgcgtccgg
     8641 tggactggcg ggcagtacgc tgtctgtcga tgcggcagtg cgcaacatgg tcgagttgac
     8701 gggcgtaacg cctgcggaag ccatccatat ggcgtcgctg catccggcgc gtatgctggg
     8761 tgttgatgat gttctgggat cgcttaaacc gggcaaacgc gccagcgtcg ttgcgctgga
     8821 tagcgggctg catgcgcaac aaatctggat tcagggtcaa ttagcttcgt tttgatggtt
     8881 tgctccttta ttgggccttc acttcccccg taaggccttt ctttttcttt cgttttgatc
     8941 tgtgcagcgg tgtcggatgc gacgctaatg cgccttatcc gacctacggt tggtgaccgt
     9001 aaggccggat aaagtattta cgccgcatcc agcaatccct tttacttccc ttgtcttttc
     9061 tttcaacgat cacagatttc gttttatttc ctttttctcc attgaacttt cagtttcttt
     9121 tctatagatt ttaaccaacg aaagaaatca ccaagtgaaa tgaaacgaaa ggcaaacgaa
     9181 agcgacaacg ccgacgtcaa gttcatcaga ctaaggattg agttatgcca gaaaattaca
     9241 cccctgctgc tgccgcaacc ggtacatgga ctgaagaaga gatccgccat cagcctcgcg
     9301 catggatccg ttcactcacc aacatcgacg cgctacgttc cgcgctcaat aacttccttg
     9361 aaccgttact gcgcaaagag aatctgcaaa tcatcctgac cggagccgga acatcggcat
     9421 ttatcggtga catcatcgcg ccgtggctcg ccagtcatac cggtaaaaac ttcagcgccg
     9481 taccgaccac cgatctggtc actaatccga tggactacct gaatccagct catccgctgc
     9541 tgttgatctc cttcggtcga tccggcaaca gcccggaaag cgtcgccgcc gtggaactgg
     9601 caaatcaatt tgtacccgag tgctatcacc tgccgatcac ctgcaacgaa gcgggcgctc
     9661 tttaccaaaa cgcgatcaac agcgacaacg cgtttgccct actgatgccc gcagaaacgc
     9721 acgaccgcgg ctttgcgatg accagcagca ttaccaccat gatggccagt tgcctcgcgg
     9781 ttttcgcacc tgagacgatc aacagccaga gcttccgcga cgtggcggat cgttgccagg
     9841 cgatcctgac ctcactgggc gatttcagcg aaggtgtgtt tggttacgca ccgtggaaac
     9901 ggatcgttta tctcggcagc ggtggcttac agggcgcagc acgcgagtcg gcgctgaaag
     9961 tgctggaact gacagcgggt aaactggcgg ccttctatga ttccccgacc ggattccgtc
    10021 atggcccgaa atcgctggtc gataacgaaa cgctggtggt ggtatttgtc tccagccacc
    10081 cttacacccg tcagtatgat cttgatctgc tggcagaact ccgccgcgat aaccaggcaa
    10141 tgcgcgtaat cgccatcgcc gcggaaagca atgacgttat tactgccggt ccacatatca
    10201 tcctgccgcc gtcccgtcac tttatcgacg ttgagcaggc attttgcttc ctgatgtacg
    10261 cccagacatt tgcactgatg cagtcgctgc acatgggtaa tacaccggat accccatcag
    10321 ccagtggcac cgttaaccgc gtggtgcaag gcgtaatcat tcatccgtgg caggcataag
    10381 aggatcgcat tatgagcatt atctccacca aatatctgtt acaggacgcc caggccaatg
    10441 gctacgcggt gcctgccttt aacattcaca acgccgagac gatccaggcg atcctcgaag
    10501 tgtgcagtga aatgcgatca ccggtgatcc tcgccgggac gccggggacc tttaagcata
    10561 tcgcgctgga agagatctac gccctgtgta gcgcctattc cacaacctac aacatgccgc
    10621 tggcactgca tctcgaccac cacgaatcgc tggatgatat tcgccgtaaa gtccacgcag
    10681 gcgtgcgcag cgcgatgatc gacggcagtc acttcccgtt tgccgagaac gtgaagctgg
    10741 tgaaatcggt tgtcgacttc tgccactccc aggattgcag cgtggaagca gaactgggcc
    10801 gcctcggcgg tgttgaagat gacatgagcg ttgacgccga aagtgcattc ctgaccgatc
    10861 cacaagaagc taaacgcttt gtcgaactga ctggcgtcga cagcctggcg gtagcgattg
    10921 gtacggcgca cggcttatac agcaaaacgc cgaagattga tttccagcgg ctggcggaaa
    10981 ttcgtgaagt ggtggatgtt cctctggtgc tgcatggtgc cagcgatgtc ccggatgaat
    11041 ttgtccgtcg cactattgaa cttggcgtca caaaagtgaa cgttgccaca gaattaaaaa
    11101 tagccttcgc tggcgcggtt aaagcctggt ttgcggaaaa tccacagggt aatgatcctc
    11161 gttattatat gcgcgtcggt atggatgcga tgaaagaagt tgtcagaaat aaaattaatg
    11221 tctgtggttc agcgaatcga atttcagcat aatcattagt tttttatgat ttatcccaat
    11281 gtacttcccg attaatatcg gggagtgcct taatggtaaa ggagataact aaaccttaat
    11341 aaatacatca gtaaaatatc gcaacaataa tatatttaaa aaaattatat tattcaactt
    11401 tatggtgagg atcacacaat gagcagtcca aatattctct taacccgtat tgataaccgt
    11461 ctggttcacg gtcaggttgg cgtgacctgg acatccacca tcggtgcgaa tctgctggta
    11521 gtcgtggatg atgttgtcgc taacgatgat attcaacaga aattaatgag tattaccgca
    11581 gaaacctacg gctttggcat tcgtttcttt actatcgaaa aaaccattaa cgtcatcggc
    11641 aaagctgcac cgcatcaaaa gatcttcctg atttgccgta cgccacaaac ggtacgtaaa
    11701 ttggtagaag gtggtattga cctgaaagat gtcaacgtcg gcaatatgca tttctcggaa
    11761 gggaaaaagc aaatcagcag taaagtttat gtcgatgacc aggatctcac ggacttacgt
    11821 tttattaaac aacgtggcgt gaatgttttc attcaggacg tccctggcga tcaaaaagaa
    11881 caaatccctg actaaatcta aaatcgcctt aatattggtt tgaggtaata aaaatgcatg
    11941 aaataaccct acttcaggga ttatccctgg cggcgttagt tttcgttctg gggattgatt
    12001 tttggctgga agccttattt ttattccgcc cgataatcgt ttgtacccta actggcgcta
    12061 ttctcggtga tattcagact ggcttaatta ccggtggtct gacagagttg gctttcgccg
    12121 gattaacccc tgcaggtggt gttcagccgc ccaacccgat tatggcgggt ctgatgacca
    12181 ccgtcattgc atggtctacg ggcgttgatg ccaaaacagc aattggtctt ggcctgccgt
    12241 ttagtttgtt aatgcagtat gtcattctgt tcttctattc tgctttctca ttatttatga
    12301 ccaaagccga taaatgcgcg aaagaagcgg atacggcagc gttttcccga cttaactgga
    12361 caacgatgct catcgttgct tcagcgtatg cggtgattgc tttcctctgt acttacctgg
    12421 cacaaggggc gatgcaggcg ctggtgaaag cgatgcccgc ctggctgacc cacggctttg
    12481 aagtggctgg cggtattctg cctgccgttg gttttggctt gctgctgcgc gtgatgttca
    12541 aagcgcaata tatcccttac ctgatcgccg gcttcctgtt tgtttgctac atccaggtca
    12601 gcaacctgtt gccggttgcc gtactgggcg caggctttgc ggtgtatgag tttttcaatg
    12661 cgaaatcccg gcagcaagcg caaccgcagc ccgttgccag taaaaatgaa gaagaggact
    12721 acagcaatgg gatctgaaat cagtaaaaaa gatatcaccc gtctgggctt tcgttcgtca
    12781 ctgctacaag caagctttaa ctacgaaagg atgcaggcgg gcggctttac ctgggcgatg
    12841 ttgccgctcc tgaaaaagat ttataaggac gacaaaccag gcctaagcgc ggcgatgaaa
    12901 gataacctag aatttattaa tacccacccg aatctggtcg gattcctgat ggggttatta
    12961 atttcgatgg aagagaaagg ggaaaaccgc gacaccatta aaggcctcaa agtggcgctg
    13021 tttggcccaa tcgccgggat tggcgatgcg attttctggt ttaccttgtt accaattatg
    13081 gcggggattt gctcatcgtt tgccagtcag ggaaacctgc tggggccgat tctgttcttt
    13141 gccgtttacc tgcttatctt tttcttgcgc gtcggctgga ctcacatcgg ttactcggtc
    13201 ggcgtgaagg ctattgataa agtgcgagag aactcgcaga tgattgcccg ttcggcaacc
    13261 atcctcggga tcacggtaat cggcgggctg atcgcttcgt atgtgcatat taacgtggtg
    13321 acatcgtttg ccatcgacag tacccacagc gtcgcgctgc agcaggattt cttcgataaa
    13381 gtcttcccga acattttacc gatggcctac accctgctga tgtattactt cctgcgggtg
    13441 aaaaaagcgc atccggtgct gttaatcggc gtgacgtttg ttctttctat tgtttgttct
    13501 gcattcggca ttttgtaaat ggaacgaggc accgcgtctg gcggtgcctc tttacttaag
    13561 gaatttcatc ctgtgcaaac ccttcaacaa gttgaaaact atacggcgtt aagtgaacgt
    13621 gccagcgaat atttattggc cgtgatccgt agcaaaccgg atgccgtgat ttgcctggcg
    13681 accggagcca cgccattact gacgtatcat tatctggtag aaaaaatcca ccagcagcag
    13741 gttgatgtca gccagctcac cttcgtgaag ctcgacgaat gggtggatct gccattaacg
    13801 atgcctggta cctgcgaaac tttcctgcaa cagcatatcg tgcagccgct ggggctacgt
    13861 gaagaccagc tcatcagctt tcgctccgaa gagattaatg agacagagtg cgaacgggta
    13921 acgaacctga ttgcgcgcaa aggcggtctg gatttatgcg ttctcgggtt ggggaaaaac
    13981 ggtcatcttg ggctgaacga accgggagaa agcctgcaac cggcctgcca tatcagtcaa
    14041 cttgatgcca gaacacagca acatgagatg ttaaaaaccg cgggccgtcc tgtgactcgt
    14101 gggatcacct taggcctgaa ggatattctc aatgcccgcg aagttttgtt actggtgact
    14161 ggcgaaggaa agcaggatgc gacagaacgt tttctcacgg ctaaagtctc taccgctatc
    14221 ccggcttcat ttttatggct gcataactat ttcacatgca tgattgatga gatgtgtcgt
    14281 cgttaactcg cctgctacag gcgttgcaac gctattcctg ctgcgacgcc tgtaatcact
    14341 ctccctgctg ctccagcgca tacttataca gcgcattttt cttcacgccg tgaatctctg
    14401 ccgccagcgc cgccgctttt ttcagcggca gttctgcctg tagcagcgcc agcgtgcgca
    14461 gggcatcggc gggtaagtct tcttcctgcg ctttatggcc ttcgacaatc agcaccattt
    14521 cgcctttgcg acggttttca tcttctttga cccacgccag cagctcgcca acgggcgcgc
    14581 cgtgaatggt ttcccaggtt ttggtcagct cacgcgccag aaccacgtag cgggattcgc
    14641 ctaataccgc aacgatatct tccaagctat ctaacagacg gtgggtagat tcataaaaaa
    14701 tcagcgtgcg cggctccgct tcaatagctt ttagcgcatc acggcggcct tttgatttgg
    14761 caggtaaaaa gccttcataa caaaaacgat cggatggtaa acccgcggcg cttaacgcag
    14821 tgatagcagc acacggccct ggtagcggca ccacgcgaat ccccgcttca cgacaggtac
    14881 gcaccagatg gtagccagga tcgttaatta gcggcgttcc ggcatcggaa accagcgcaa
    14941 tgttttgccc ctcttgcagt ttcgccagca gcgtttcggc tttttgttgt tcgttatggt
    15001 cgtgcagcgc aaacaaccgg gcattaatcc caaaatgttg cagcaataaa ccggtgtgac
    15061 gcgtatcctc ggcggcaatc agatcaacgg cctgtaatac ctctaatgca cgctgggtga
    15121 tatcagccag attgccgatt ggcgtcggta caatgtaaag ctggccctga gaattatccg
    15181 ccgattggtg ttgtttcatt gtgtcgtccg tattgccgat ttaatattga gcattgcgta
    15241 aaaaaaatat cactggatac attatggtac cctcaacatt ttctcgtttg aaagccgcgc
    15301 gttgtctgcc tgttgttctg gcagccctga ttttcgccgg ttgtggcacc catactcccg
    15361 atcagtccac tgcttatatg cagggcacgg cgcaggctga ttctgccttt tacctgcaac
    15421 agatgcagca aagttctgat gataccagga tcaactggca attactcgcc attcgtgcac
    15481 tggtgaaaga aggtaaaacc ggacaggcgg tggagttgtt taaccaacta ccgcaagaac
    15541 tgaacgattc tcagcgtcgc gagaaaacac tgctggcggc agagattaaa ctggcgcaga
    15601 aagattttgc tggcgcgcaa aacttgctgg cgaaaatcac acctgccgat ttagaacaaa
    15661 accagcaagc ccgttactgg caggcaaaaa tcgatgccag ccaggggcgt ccttccattg
    15721 atttactgcg cgcgttaatt gctcaggaac cgctgctcgg tgcgaaagaa aagcagcaga
    15781 atattgacgc cacatggcag gcgctctcct ccatgactca ggagcaggcg aatacgctgg
    15841 tgatcaacgc cgacgaaaat attctgcaag gctggctgga tttgcagcgc gtctggtttg
    15901 ataaccgtaa cgatcctgac atgatgaaag ctgggatcgc cgactggcag aaacgttatc
    15961 cgaacaaccc gggcgcgaaa atgctaccaa cgcagttggt taacgtaaaa gcgtttaaac
    16021 ccgcatcgac caacaaaatc gccctgctgt tgccgctgaa tggccaggca gcggtatttg
    16081 gtcgcactat tcagcaaggc tttgaagcgg cgaaaaatat cggcactcag ccagtggcgg
    16141 cccaggtagc agccgcacct gccgcagacg tagcagaaca acctcagccg caaaccgtgg
    16201 atggcgttgc cagcccggca caagcttcgg ttagcgagct gaccggtgaa cagcctgcgg
    16261 cccagccggt acctgtaagc gccccggcga caagcaccgc agcggtaagc gcacccgcaa
    16321 atccatccgc agagctgaaa atctacgata cctcatcaca accacttagc cagatcttaa
    16381 gccaggttca gcaggatggt gcgagtattg tggtcggtcc gttgctgaaa aataacgttg
    16441 aagagttgct gaagagcaac accccgctga acgtgctggc actgaaccag ccggagaaca
    16501 tcgaaaaccg cgtcaatatt tgttacttcg cgctttcacc ggaagacgaa gcgcgcgatg
    16561 cagcgcgtca tattcgtgac cagggtaaac aagcgccgct ggtgctgatc ccacgcagtt
    16621 cattgggcga ccgcgtagcc aatgcgtttg cgcaagagtg gcagaaactg ggcggcggca
    16681 ccgttctgca acaaaaattt ggttccacca gcgaattacg cgcgggtgtt aacggcggtt
    16741 ctggtattgc tttaacgggt agcccgatta ctcccagagc gacaaccgac tccggcatga
    16801 cgaccaacaa tccaacgctg caaaccacgc caaccgatga ccagttcacc aataatggcg
    16861 gtcgtgtcga tgcggtgtac attgtggcaa cgccgggtga aatcgctttt atcaaaccga
    16921 tgatcgccat gcgtaacggt agccagagcg gtgcaacgct gtacgccagt tcccgcagcg
    16981 cacaaggcac cgcaggcccg gatttccgtc tggagatgga aggcttgcag tatagcgaaa
    17041 tcccgatgct ggcaggcggt aatctgccgt taatgcagca ggcgctcagc gcggtgaaca
    17101 acgattattc gctggctcgc atgtatgcga tgggcgtcga tgcctggtcg ttggcaaatc
    17161 atttctcaca aatgcgccag gttcagggtt ttgaaatcaa cggtaatacc ggaagtctga
    17221 cggctaaccc ggattgcgtg attaacagga agttatcatg gctacagtac caacaaggtc
    17281 aggtagtccc cgccagttaa ccaccaaaca gaccggcgat gtgtgggaag cacaagcgcg
    17341 tcgctggctg gaaggcaaag gactgcggtt tatcgccgct aacgtgaacg agcgtggcgg
    17401 cgagatcgat ctgataatgc gtgaaggccg gatcaccgtt tttgtcgagg tgcgttaccg
    17461 ccgctctgcg ctttatggcg gcgcggcagc cagtgtgacc cgcagcaaac aacacaaatt
    17521 attacagact gcccgcttgt ggctcgcgcg tcataatggg agttttgata ctgtggattg
    17581 ccggttcgat gtggtagcct tcaccgggaa tgaggttgag tggattaagg atgcctttaa
    17641 tgaccactca taattaaggt ttaaggatta gcgtgcaaga aagaattaaa gcttgcttca
    17701 ctgaaagcat tcaaactcaa attgcggcgg cagaggcgct tccggatgcc atctcgcgtg
    17761 cagccatgac gctggttcag tctctgctca atggcaacaa aatcctctgt tgtggtaatg
    17821 gaacttccgc tgccaatgca cagcattttg ctgccagcat gatcaaccgt ttcgaaacgg
    17881 agcggcccag cttacctgcc attgcactaa atactgataa tgttgtctta acggcgattg
    17941 ccaacgatcg cttacatgat gaagtgtatg caaaacaggt gcgggcgctg ggtcatgcgg
    18001 gagatgtatt gttagccatt tccacccgtg gcaacagccg cgatattgta aaagcggtag
    18061 aagctgccgt tacgcgtgat atgaccattg tggcattaac cggctatgac ggcggtgaac
    18121 ttgcaggttt gttagggcca caggatgtgg agatccgcat tccttcgcat cgtagtgctc
    18181 gcattcagga aatgcatatg ctgacggtaa attgcctgtg cgatctgatc gataacacgc
    18241 ttttccctca ccaggatgtt taaggagaat acatgaaggc attatcgcca atcgcagtcc
    18301 ttctttccgc gctgctgttg caaggttgtg ttgccgctgc cgtagtgggt accgctgctg
    18361 taggtaccaa agccgcaact gacccacgca gtgtcggcac ccaggtggac gatggtaccc
    18421 tggaagtgcg cgtgaacagc gcattgtcga aagacgaaca aattaagaaa gaagcgcgca
    18481 ttaatgtcac ggcctatcag ggcaaagtgc tgctggttgg gcagtcacca aacgctgaac
    18541 tttcggctcg cgccaaacag attgctatgg gcgtagacgg tgccaacgaa gtgtataacg
    18601 agattcgtca gggccagccg attggtctgg gcgaagcgtc taacgatacg tggatcacca
    18661 ccaaagtacg ttcgcagctg ttaaccagcg acctggtgaa atcgtccaac gtgaaagtga
    18721 ccaccgaaaa cggtgaagtg ttcctgatgg ggctggtgac tgaacgtgaa gcgaaagcgg
    18781 cggcagatat tgccagccgg gtaagcggcg tgaagcgggt aactacggcg tttacgttta
    18841 ttaaatagca gcccttgtaa tgcctgatgc gacgcttgca gcgtcttatc aggcctacaa
    18901 cgatgcaaac cgtaggtcgg ataaggcgtt tacgccgcat ccgacatgtt ttccctcaaa
    18961 tcaaaccaac agcgccagcc cgccgacaat cacaccagac actgccacca tcgcccccgt
    19021 cagccataag gctttcgccg ggaacgcttt gcgcagcata atcagtgacg gcaaactcac
    19081 cgccgggagc gtcatcaaca atgccagcgc cggagcagtt cccatacctg ccagcatcat
    19141 cgtttgtaca atcggaattt ctgctgccgt gggaatgaca aacaagcatc ccgctaccgc
    19201 catcgccacc acccacatca ggctgttatc gacagcacca tcggcatggg ggaataacca
    19261 gacgcgagcg gcacccaaca ccagtactgc aaggatgtaa accgggatcg tactccagaa
    19321 aagcgtccat agcgccctgc cccagcggct aaaaaatcct ccctgtgctt ccggtatgtc
    19381 aatttcgacc ggtgcctgcg tttgcggtgt ttcacgcacc catttttgca ccagcgtcgc
    19441 aatcagcaac accatcacca gccccgccac cagacgaatc gccgcaaaac cccagccgag
    19501 gacgaacccc ataaacacca gcgtcgccgg gtttaacaac ggattcccca tccagaatgc
    19561 cagcgcaccg cccatcgaca cctgttggcg acgcattccc gccgcgaccg gagccgcaca
    19621 gcaggtacac atcatgcccg gcagcgaaaa cagcgttccc agcagcgtgc cgcgaaagcg
    19681 cgattgccca agcgtacgca ataaccagtc acgcgggatc agcacctgaa tcaacgaccc
    19741 gagcagtacg cccagcaccg ccgctttcca taccgcgagg aaatagatca tcgcgtaatc
    19801 caacgccgcc tgccacggat tagcatccgc ctgcgcaagg atagatttac cgatactgtg
    19861 ggtttcggca gcagtaaacg ctttgccgta gtaaggttgc catttcacat accagagacc
    19921 agcaatgaca acgagaaaga aaagcgcggg tttccaccac tgaatgggcg ttgccgcctg
    19981 agatgaagac tgaccagtca tagcattccc caggaataag ttatgataat tgagcgcgtg
    20041 aatattacgc tcactatcaa ttcttgggaa taattattca gctcttttgc gtaattctga
    20101 agagcttaaa atcgtcacac cttcatgctc aggccgcatc gcctctgcca gcatgacccg
    20161 cgccacgtct ctcgcatcaa tggatttcca gttacccggt aacaaacgga acagcggcgc
    20221 aaaaagcgtt tcgttcatcc gctgtttgct acgatcgccc agtaacatcg acgggcgagc
    20281 aatggtcaat ttcggccagt tctgggcgat taatgcttct tccatctccc ctttgacgcg
    20341 gttatagaaa aacggcgagt gggcattagc acccatagca ctgaccacca gcatatgctg
    20401 cgcaccgagc cgccgcccgg ttaatgcggt atccaccacc agcgtgtaat cggcatgaat
    20461 aaacgcctct ttactccccg cttctcgccg cgtggtgccg agacaacaaa acacaatgtc
    20521 gatgggatcg gtgacctgcg ccagcgcgtc ggtcagttgc ggatcatggg gattaaacac
    20581 accgggcata tcgcccaacg gacgtcgcgt cggcgcggct atggcgttaa ctttcggttc
    20641 attaatcaac atccgcagca ggtgaccacc caccagcccc gttgcgcctg taatcagtac
    20701 ctggctcatc ttcgctcctt tacagaattg tctgccttgc gctccccggc tcatgcacca
    20761 ggcttaatag tctacgaggt aattatcccc gattgtggga aattcgcctc atccaatgca
    20821 acaacgcgga ggaagcatga gtaagaaaat tgccgtttta atcactgatg aatttgagga
    20881 ttcagaattt acttcacccg cagacgagtt ccgtaaagcc ggacacgaag tgattaccat
    20941 tgaaaaacaa gcgggtaaaa cggtgaaagg caaaaaagga gaagccagcg tggccatcga
    21001 taaatccatc gatgaagtga cgccagcgga gtttgatgcc ctgctgctac cgggcggcca
    21061 ttcaccggat tatctgcgtg gcgacaaccg ttttgtcact ttcacccgtg attttgtgaa
    21121 tagcggcaaa cctgttttcg ccatctgtca cggtccgcag ctgctgatca gcgccgatgt
    21181 gattcggggg cgcaaactga ctgccgttaa accgatcatt attgatgtta aaaacgcggg
    21241 cgcggaattt tacgatcagg aagtggtggt cgataaagat cagctggtta ccagccgtac
    21301 accggacgat ctgccagcgt ttaaccgcga ggcattacgc ctgctcggtg cctgagttac
    21361 gtagccagat cattttttta ccaaagccca gcgtgttgtc ggtgaatttg atttcatcga
    21421 gccggatttc ccacaccggg gccgacagca ttctggcaac cggaaagcgg cgattgtacg
    21481 cctggcgcgc aaggtcgctt tcctcacctt caagcctgcg gatctcgcct ttaaattgca
    21541 cgccgcgaat taacgctacc gttttcggct gaccgtttac cgttccggca actgccgcct
    21601 gcggcccact catctgcgcg tggcgcgttt tttcttccgt caaaatgtag aaggcgactt
    21661 tctgcgcatc aaaaagataa aaggcattag cgcaccagag ttccccttcc tgctgcacac
    21721 accaggtgac aacatgttgt tttgccagcc aacggctgat ggcggtgagt gtttccattg
    21781 ctgttctctt ttatactgtg ggcctgacct taacatgcga cgcagacgat gacaccctgg
    21841 tttctttact tgatccgtac cgccgacgat aagctttata ccgggatcac cacagatgtc
    21901 gaacgccgct atcagcagca ccaaagcggc aaaggggcga aagcactgcg cgggaaagga
    21961 gaactaacgc tggcgttttc cgcgccagtg ggcgatcgtt cgctggcact acgggcggaa
    22021 tatcgcgtta agcaactgac caaacggcaa aaagagcgtc tggtagcgga aggcgcaggg
    22081 tttgcagaac tgttgagcag cctgcaaacc ccaaagatta aaagcgattg aagtgctcgt
    22141 gatactcaac cagaccagtt acaccgttca gcgcgtcatc cgctaaacga tgtacctgga
    22201 aggcactttc agtgcccggc cagcggcaac gcagatcgtg atgtgccgcc agttcaaagc
    22261 cgaaacggct gtacagcgcc ggatcgccca gcgtcaccac tgcggcatag ccgaactcat
    22321 taagcgaatc gagtccttca tagaccagtt ggcgtgccag cccctgtcca cggtattttt
    22381 catctaccgc cagcggtgcc atgccgaccc attgcaggtc ttcgccctgc acatcaaccg
    22441 gactaaatgc cacatagcca atgacctgac cttcgtcatc tgtcgccacc agccccagcg
    22501 tcagaaagcc atcttcacgc agatcgtgaa ccagcttcgc ttccgcatca ctttcgaatg
    22561 aacgacgcag cagggcatca atacccggcg catcaatggg aatttctact cgaattagca
    22621 tggttcacct accgatgtct gtttggtttc aggcgcggtt ttcattcccg cctcaacaaa
    22681 atccgccagt tgcagcagca tcatgcgtaa ggctttcggc atttgctcca gttcaatggc
    22741 gtccatcagg tttttcacat acagccccag ctccgtatcg ccttcaatta ccagccgacg
    22801 ctggaagaag agcgtatccg gatcttgttt acgcgccgca atcatcagca gatcgctggc
    22861 gtcggcacta aaactcacat cagcttgcgc gttctggcta acgaccagtt tgccattcac
    22921 caccgaggta aaccattgca ggtcaatatc acgcacatga atacttaacc agcggccttc
    22981 aagaaactcc agctcgccat catccaacgc ctggcggaat tgccagctta agacctgctc
    23041 aagaacctgg cgttttagcg caaatggcgt cagttttacc ggtacactca acagagatgg
    23101 ccccaaatgc acaatacggg aacgcagttt atccaacacg agttttactc cctgtttcaa
    23161 caatcatcct attttgccat atcagaaaaa taacatagcg gtataaatca acaattccat
    23221 atgaaattgc tgctaccacc aatacaactt taactgcctt aaatcaaaaa ttgtcgcagc
    23281 aaggttaact aaaatcccag ttcgttaaca tttttgcgct ttgatagcgc aaccttcagg
    23341 aaaaattatg gagctgctct gccctgccgg aaatctcccg gcgcttaagg cggccatcga
    23401 aaacggcgca gatgctgttt atatcgggct aaaagatgat accaatgccc gtcacttcgc
    23461 cggccttaac tttaccgaga aaaaattgca ggaagcggtg agttttgtcc atcaacatcg
    23521 ccgcaaactt cacatcgcga ttaacacttt tgcgcatccg gacggttacg cccgctggca
    23581 gcgcgccgtg gatatggcgg cgcagttggg tgccgacgcg ctgatcctcg ccgacctcgc
    23641 catgctggag tacgccgccg agcgttaccc gcatattgag cgtcatgtgt cagtgcaggc
    23701 ttcggcgacc aatgaagagg cgattaactt ttatcatcgc cattttgacg ttgcccgcgt
    23761 ggtgctgccg cgcgtgttgt cgattcatca ggtgaaacaa ctggcacggg tcacacctgt
    23821 cccgctggaa gtgtttgctt tcggcagcct gtgcattatg tcggaaggcc gttgctatct
    23881 gtcgtcgtat ctgacgggtg agtcgcccaa caccgtgggc gcgtgttctc cggcccgttt
    23941 cgtgcgctgg cagcaaacgc cgcagggact ggaatctcgc ctgaacgaag tgctgatcga
    24001 ccgttatcag gatggcgaaa acgcaggtta tccgacgctg tgtaaagggc gttatctggt
    24061 ggacggcgag cgctatcacg cgctggaaga acccaccagt ctcaataccc tggaactgct
    24121 gccggagtta atggcggcga atattgcttc ggtgaaaatt gaaggccgcc agcgtagccc
    24181 ggcgtatgtc agccaggtgg cgaaagtctg gcgtcaggct atcgaccgtt gtaaggccga
    24241 tccgcaaaac ttcgtaccgc aaagcgcgtg gatggagacg ctcgggtcga tgtccgaagg
    24301 cacgcaaact acccttggcg cgtatcaccg taaatggcag tgagaaaagc aatgaaatat
    24361 tccttagggc cggtgctatg gtactggcca aaagagacgc tggaagactt ttatcagcag
    24421 gccgccgcca gcagcgcaga cgtgatttat cttggtgaag cggtctgtag caaacgccgg
    24481 gcaaccaaag ttggcgactg gctggagatg gcaaaatcgc tcgccggaag tggcaagcag
    24541 attgtgctct ccacgctggc gctggtgcag gcgtcctccg aacttggcga actgaaacgt
    24601 tatgttgaga acggtgagtt tctgattgaa gccagcgatc tcggcgtggt gaatatgtgc
    24661 gctgaacgca aactgccgtt cgtcgccggg cacgcgctga actgctacaa cgcggtgaca
    24721 ctgaaaatat tgctcaaaca gggcatgatg cgctggtgta tgccggtgga gctttcccgc
    24781 gactggctgg tgaatctgct taatcagtgc gatgagctgg gcattcgcaa ccagtttgaa
    24841 gtggaagtcc tgagctacgg tcatctgccg ctggcctact ccgcccgctg ctttaccgcg
    24901 cgttcggaag accgcccgaa agatgagtgt gaaacctgct gcattaagta tccgaacggg
    24961 cgcaacgtgc tatcgcagga aaaccaacaa gtgtttgtac tcaatggcat tcagaccatg
    25021 agcggctacg tttacaacct cggtaacgag ctggcatcca tgcaggggct ggtcgatgtg
    25081 gtccgcctgt caccgcaggg tactgacact ttcgcgatac tcgacgcctt ccgcgccaat
    25141 gaaaatggcg ctgcaccact gccgttgacg gcaaacagcg attgtaacgg ctactggcgg
    25201 cggctggccg ggctggagct gcaagcctaa gtaaatagct cactttgtta acaactttaa
    25261 ctactcttta atgcagtatt aaagattaat cagtaacaaa gtgagctgtt atgactgata
    25321 aaaccattgc gttttcgcta ctcgatctgg cccccattcc cgaaggctct tcagcgcggg
    25381 aagcgttctc ccactctctc gacctcgccc gtctggctga aaagcgcggc tatcatcgct
    25441 actggctggc ggaacaccac aatatgaccg gcattgccag tgctgcaaca tcagttttga
    25501 ttggctatct ggcggcgaat accactacgc tgcatctggg gtctggcggc gtgatgttgc
    25561 ctaaccactc accattggtg atcgccgaac agttcggcac gcttaatacg ctctatccgg
    25621 ggcgaatcga tttggggctg ggtcgtgcgc cgggtagtga ccagcgaacc atgatggcac
    25681 tacgtcgtca tatgagcggt gatattgata atttcccccg cgatgtcgcg gagctggtgg
    25741 actggtttga cgcccgcgat cccaatccgc atgtgcgccc ggtaccaggc tatggcgaga
    25801 aaatccccgt gtggttgtta ggctccagcc tttacagcgc gcaactggcg gcgcagcttg
    25861 gtctgccgtt tgcgtttgcc tcacacttcg cgccggatat gttgttccag gcgctgcatc
    25921 tttatcgcag caacttcaaa ccgtcggcac gactggaaaa accatacgcg atggtgtgca
    25981 tcaatattat cgctgccgac agcaaccgcg acgctgaatt tctgtttacc tcaatgcagc
    26041 aagcctttgt gaaactacgc cgtggcgaaa ccggacaact accgccaccg attcaaaata
    26101 tggatcagtt ctggtcaccg tctgagcagt atggcgtgca gcaggcgctg agtatgtcgt
    26161 tggtaggtga taaagcgaaa gtgcgtcatg gcttgcagtc gatcctgcgc gaaaccgacg
    26221 ccgatgagat tatggttaac gggcagattt tcgaccacca ggcacgcctg cattcgtttg
    26281 agctggcgat ggatgtgaag gaagagttgt tggggtagtg tgttttaacg taggaagcct
    26341 tatccgagat ggcaacgctg tcctacgtag gcctgataag cgaagcgcat caggcattgt
    26401 gtaggcagca gaaatgtcgg ataaggcacc gctgattact gatacaccgg cagcaaatta
    26461 aagctcgata agatatggac cagtgcgttg ccaacgccaa ataccagaat cagcataatc
    26521 atcggcttgc cgccccagac gcggaatttc gggctaccaa agcgtttacg cgatgcgcgg
    26581 gccaacagcg ccggaacaat tgccgcccag atggtagccg ctaagccagc ataaccaatg
    26641 gcgtacagga atccgttagg aaacagcagg ccacccacaa caggcggggc aaaggtcagc
    26701 aacgctgttt tcaaacggcc catagccgag tcgtcgaaac caaacagatc tgccagatag
    26761 tcaaacaaac ccagcgttac gccaaggaac gaactcgcta ccgcaaagtt tgagaacacg
    26821 accagcagaa gatccaggct acggctgttc agcacgccgc ttaatgcctg taccagcaca
    26881 tcaatattac cgcccttctc ggcgatgccg ataaactccg gacgagggat gttgcccatc
    26941 gtcgccagca accagatggt atacagcgcc agcgccatca gcgtaccgta caccaggcat
    27001 ttcacgatgg ttttcggatc tttgccgtaa tacttcatca ggctcggcac gttaccgtga
    27061 taaccaaacg atgccagaca gaatggcagt gtcatcagca gatacggcgc ataagacgca
    27121 ttgctttcgg cgacgttgaa caaggtcgtt ggctgtacat gccccagcag gctgccgaag
    27181 gtgaggaaga aagtaatgac tttcgccccc agcacaattg ctgtcatccg gctgacagct
    27241 ttcgtactca accacaccac gaacgctacc agcagtgcaa aaccaaatcc agctgcccgt
    27301 gcagggacgt tcagcgacat ctccgcgaag gtgtgatgca gaatcgaacc gctggcagaa
    27361 atataggcgt aagtcaggat atagagcaca aaggcaatgg aaatgccgtt gactacgttc
    27421 cagcctttgc ccagcaaatc tttggtgatg gtgtcaaagc tagaaccgat tcgataattc
    27481 aggttagctt ccagaatcat caagccggaa tgcagcatac agaaccaggt aaagatcagc
    27541 gccgccattg accagaagaa ccacgccccg gacatgacca caggcagaga aaacatccct
    27601 gcgccaataa tggtgccgcc gataatcacc acgccgccaa gcagcgacgg tgacgtttgg
    27661 gtggtggtta gtgttgccat gagggcttct ctccagtgaa aaatagtgcg actgcgttgt
    27721 tatgcattgc actgtaccag tacacgagta caaaagacag aaaaaaagcc ccgatggtaa
    27781 aaatcggggc tgtatatatt attttacaga ttgtgttcgc tgttcagcga tgattacgca
    27841 tcaccaccga aacgacgacg accggtagag tcatcacgac gcggagcgcg gccttcacga
    27901 cgttcgccgc taaaacgacg accatcacca cgaccacctt cacggcgttc accgctgaag
    27961 ttacgaccgc cttcacgacg ttcgccactg aaaccacgac caccgccacg acgctcaccg
    28021 ccagtatgcg gctgtgcatc gcccagtaac tgcatgttca tcggcttgtt gagaatgcga
    28081 gtgcgcgtaa agtgttgcag cacttcaccc ggcatacctt tcggcagttc gatggtggag
    28141 tgagaagcaa acagcttgat gttaccaatg taacggctgc tgatgtcgcc ttcgttagca
    28201 atcgcaccaa cgatatgacg aacttcaaca ccatcatcgc ggcccacttc aatgcggtac
    28261 agctgcatat cgccaacatc acgacgttca cgacgcggac gatcttcacg gtcaccacgc
    28321 gggccacggt cgttacgatc gcgcggacca cggtcatcac ggtcacggaa ttcacgtttc
    28381 ggacgcatcg gcgcatctgg cggaacaatc agagtacgtt caccctgtgc cattttcagc
    28441 agtgcggcgg ccagagtttc gagatccagc tcttcacctt ctgccgtcgg ctgaattttg
    28501 ctcagcagtg cacggtattg atccagatcg ctgctttcca gctgctgctg tactttagcg
    28561 gcgaattttt ccagacgacg tttgcccagc agttctgcgt tcggcagttc aacttccgga
    28621 atggtcagct tcatggtgcg ttcaatgtta cgcagcagac gacgctcacg gttctcaacg
    28681 aacagcagcg cacggccagc acggcccgca cgaccagtac gaccgatacg gtgaacgtaa
    28741 gactcagaat ccatcgggat atcgtagtta actaccaggc tgatacgctc aacgtccagg
    28801 ccacgggccg caacgtcggt cgcaatcagg atgtccagac gaccatcttt cagtcgttcc
    28861 agtgtctgct cacgcagcgc ctggttcatg tcaccgttca gcgcggcgct gttgtagccg
    28921 ttacgctcaa gggcttctgc cacttccaga gtcgcgtttt tggtacgaac gaagataatc
    28981 gccgcatcaa aatcttccgc ttccaggaaa cgtaccagtg cttcgttttt acgcataccc
    29041 cagacagtcc agtagctctg gctgatgtca ggacgggtag tcacgctgga ctgaatgcgc
    29101 acttcctgcg gctctttcat aaagcggcgg gtaatgcgac gaatcgcttc cggcatggtt
    29161 gcagagaaca gagcggtctg atgaccttcc gggatctgcg ccataatggt ttcaacgtct
    29221 tcgatgaagc ccatgcgcag catttcgtca gcttcatcca gaaccagacc gctcagttta
    29281 gagaggtcca gagtgccacg tttcaggtgg tccagcagac ggcccggagt accgacaacg
    29341 atctgcggcc cctgacgcag ggcgcgtaat tgcacgtcat aacgttggcc gccgtacagg
    29401 gcaaccacgt ttacgccgcg catgtgttta gagaaatccg tcattgcttc agcaacctgt
    29461 accgccagtt cgcgggtcgg tgccagcacc agaatctgtg gtgctttcag ctcaggatca
    29521 agattctgca gcagaggtaa agagaatgct gcagtttttc cgctccccgt ctgggccata
    29581 cccagaacgt cgcggccatt cagcagatgt ggaatacact ctgcctgaat tggagatggt
    29641 ttttcgtaac ccagatcgtt aagggcttca aggataggag ccttcaggcc cagatctgca
    29701 aaagtggttt cgaattcagc catgtagtac gtgtgcctca aaattaatgg cggccagtct
    29761 acataactca tcatgaaatt gatcagcaat tttcattgaa aagtgtgaac cggctcaaag
    29821 taggtgtatt aacgaacaac aacgccctca cccgttaagg tgatggcaat caaaaaagat
    29881 tacgggctga tgtgtacgtc agctattgct ggtccgattc tgccaggtca tcttggtcct
    29941 ggcccaggag cgataattcc aacaatgcgt atcggtgctc aacaaagtta tgaacgttgt
    30001 tggcaaccgc cagtttgaac agtgccgtgg cgctgtccaa atcccccaga cttaggtagt
    30061 acttacctaa atagaagttg gtttcactga gatgctcagc gagcgaggtg ttatccgttg
    30121 cgtccgcctt gagcctttcc attaacgttt gttcgctaat gttgcccagg tagaactcga
    30181 caatgttcca tccccactgt tccttatccg atttttcgaa gtgctgtttc aacacttctt
    30241 tagcctgctt ctcatcgagc ttctgctcgg cgagataaag ccacagacta cggaaaggat
    30301 cattgggatc gtcttgataa aacgccagca gatcatcttg cgctaacttg tcacgaccgc
    30361 cgtaatataa tgcgatcccg cgattcaagt gcgcgtagtt gtaagttgga tcaagctcaa
    30421 gtacagaatc aaacgcttca taggcagcat caaaattgcc tgcctgcgtt aaatatatgc
    30481 ctaagtaatt gaatacttca ggcatatccg gtcggattgc cagcgcttgc gaaaaatcgt
    30541 tacgcgctaa tgccctcaga ccgagactat catacaacac tccgcgctca tataaaagct
    30601 gtgcgcgttc gtcatcggtt aaagcccgac tggcaaggat ttgttccata cgtgccagaa
    30661 tcacttcctg ctgtaaagtc ggttgcaatg gtaccgcgag gacttcactt ttacgccagg
    30721 aagtattact gcatcctgca agcgtaagtg ctgtcgcaac gaaacaccag cgcaaaaaag
    30781 gcttcatttc ccactcccga agaccacggt tgaatgaacg tcctgttccc ggttgctaac
    30841 aaggcgtcct gcccggttaa aagccccccg ccgcagcgga gggcaaatgg caaccttact
    30901 cgccctgttc agcagccgga gcttccggtg ctgcagcagg ttgagactgc tcagtcgctt
    30961 ctttaatgct cagacggata cggccctggc gatcaacttc cagaactttc accggtactt
    31021 cctgacccat ctgcaggtaa tcggtcactt tctcaacgcg tttgtcagcg atctgagaga
    31081 tgtgtaccag accttcttta ccgccgccga tggcaacaaa tgcgccaaag tcaacgatac
    31141 gggtcacttt accattgtag acgcggccca cttcgatttc tgcagtgatc tcttcgatac
    31201 gacgaatagc atgtttcgct ttctcgccgt cggtcgctgc gattttcaca gtaccgtcat
    31261 cttcgatttc gatggtggtg ccagtttctt cggtcagagc acggataaca gaaccgcctt
    31321 taccgataac gtctttgatc ttgtccgggt tgatcttgat ggtatggata cgcggagcga
    31381 actcagagat atcgccgcgc ggcgcgttga tcgcctgttc cattacgccc aggatatgca
    31441 gacgcgcacc tttagcctgg ttcagcgcaa cctgcatgat ctctttggtg ataccttcaa
    31501 ttttgatatc catctgcaat gcagagatac cgtcacggga acccgcaact ttgaagtcca
    31561 tatcgcccag gtgatcttcg tcgcccaaaa tgtcagacag tacaacgtag ttgtcgcctt
    31621 ctttcaccag acccattgcg atacccgcaa cggcagcttt gatcggcaca cctgcgtcca
    31681 tcagcgccag agacgcgccg cacacggaag ccatagaaga ggaaccgttg gattcggtga
    31741 tttcagacac aacacgtacg gtatacggga atttgtccat atccggcatg actgccagca
    31801 cgccgcgctt cgccagacga ccgtgaccaa tttcacgacg cttcggagaa ccgaccatgc
    31861 cggtttcgcc tacagagtac ggagggaagt tgtagtggaa caggaaggtg tcagtacgtt
    31921 cgcccatcag ttcatcaaga acctgcgcgt cacgtgcagt acctagcgtt gcggtaacca
    31981 gcgcctgagt ttcaccacgg gtgaacagcg cagaaccgtg agtacgcggc agcacaccag
    32041 tacgcacatc cagaccacgg atcatatctt tttcacgacc gtcgatacgc ggttcgcctg
    32101 ccagtacgcg gctacgaaca acatttttct cgatagcgtg cagaatttca cccagttcgt
    32161 tttcgtccag ggtttcgtct tcagcaagca gtgtcgcgat ggtttcagat ttgatgacat
    32221 caacctgcgc ataacgctct tgtttgtcgg tgatgcggta agcatcgctc agacgagctt
    32281 cagccagtgc agcaacgcgc gcgttcagcg cttcgtttac cggctccggc tgccagtccc
    32341 aacgcggttt accggcttct ttcaccagtt cattgatgtt ctgaataaca acctgctgtt
    32401 gttcatgacc gaagactact gcgcccagca tctggtcttc gctcagcagt tcagcttcag
    32461 attcaaccat cagtacagcg gcttcagtac ccgcaacaac cagatccagt ttgctctctt
    32521 tcaactcgtc ctgagtcggg ttcagtacgt actggtcatt gatgtaacct acacgggcag
    32581 caccaatcgg gccattgaac ggaataccag acagagacag ggctgcggaa gcaccaatca
    32641 tcgcgacgat atccgggtta acctgcgggt taacagaaac cacggtggcg ataacctgaa
    32701 cttcgttgac gaagccttcc gggaacagcg ggcgaatcgg gcggtcaatc agacgcgcga
    32761 tcagggtttc gccttcgctt gggcggcctt cacgacggaa gaagctaccc gggatacgac
    32821 cagcagcgta ggtacgctcc tgatagttaa cggtcagcgg gaagaagtcc tgacctggtt
    32881 tggctttttt ctgaccaaca acggtaacga ataccgcagt gtcatccatg ctaaccataa
    32941 cagcggcagt agcctgacga gccatcatgc cggtttccag agtcacggtg tgttggccgt
    33001 actggaattt acgaacgatc ggattaagca atgtaatatc ctttctcttt cttagacagt
    33061 accttacggc actggtgtta atacccgatc ttctgcgcat cctcgcgact aatgacaacc
    33121 ctaacccagc tctatgtggg taaagcctct cattagccgc gcgaacctct gcagcggaag
    33181 atcattcata gcaacaatac attagtttcc agtgaattgc tgccgtcagc ttgaaaaaag
    33241 gggccactca ggcccccttt tctgaaactc gcaagaatta gcgacgcaga cccaggcgct
    33301 cgatgagctg ggtgtaacgt gctacgtctt tacgtttcag gtagtcgagc agtttacgac
    33361 gctgagaaac catgcgcagc agaccacgac ggctgtggtg atcttttttg tgctctgcaa
    33421 agtggccctg caggtggttg atctgtgcag tcagcagtgc tacctgaact tcggtagaac
    33481 cggtgtcgtt tgcgtcacga ccaaactcag aaacgatttt agctgttgct tcagtactta
    33541 gagacatttt aaaactccaa agtatataga atgaaaggac gccgatctct aattcagcga
    33601 tcccagtgta cgttacgcaa agtgttaaac aatttacgcg acgttaagcg gcagtattct
    33661 actcgtagcg acttgttatc gcaagacggt taacattacg ccgggtattc aaccaccagg
    33721 cgacgaggcg caacgcggcc ttcatcgtca atttcgccca taccgataaa cttgccgttc
    33781 tcaccctctg taacgcgaac cagtccttcc agcggcgcac cggatgtacg aactgggtta
    33841 ccatttttga agtaaacaga agacgttaac ggaagattca ccactggata gtccgaagct
    33901 ggactgtcca ttggcatcag taatggatca agtaactccg cagccggaat atcctgctgt
    33961 tcggcttgct caaccagttc acgcagatgt tccagggtca ccatccgttc aaccggatat
    34021 ttacttaccg ccagacgacg caagtaaata acatgcgcgc cacaaccgag tttttcgccg
    34081 agatcgtcaa tgatagtgcg gatataagtg ccttttgagc agtgaatttc cagctccagc
    34141 tcattgcctt catggcgaat aaacagcaat tcataaacgg taatcgggcg cgcttcacgc
    34201 ggaacttcaa tgccctgacg cgcgtattcg tacagttttt tgccctgata tttcagtgct
    34261 gaatacatcg aagggatctg ttcgatatcg ccacggaagg tatccagcgc cgccgccagt
    34321 tgctctgcac taaaggttac cggacgttct tcaacgattt gtccgtcggc atcagaagta
    34381 tcggtacgct gtccaagacg cgcaatgacc cgataacgtt tgtcggagtc cagcagatac
    34441 tgggaaaact tcgtcgcttc cccgaggcaa attggcaaca tgccggtcgc cagcgggtcc
    34501 agcgcaccgg tatgcccggc acggttggcg ttataaatcc gtttcacttt ttgcagcgca
    34561 tcgttgctgg acatcccctg cggtttatcc agcaacaaaa cgccgttaat gtcgcgaccg
    34621 cgacgacgag gacgactcat tagtcctcct tgctgtcgtc cgggttaaca cgacgttctt
    34681 cgtcatgttt gaccacgctg gtcaccaggt ttgacatgcg catcccttca accagagagt
    34741 tgtcgtagaa gaaggtcagt tccggcacga tacgcaggcg catcgctttc cccagcaggc
    34801 tgcggatgaa accagaagct tcctgcagcg ctttgatgcc cgctttaacc gcgtcttcat
    34861 ctttgtcgtt gaggaacgtt acatatactt tggcatacgc caggtcgcga gacatttcga
    34921 caccggaaac ggtggtcatc atgcccagac gaggatcttt aatttcacgc tgcaggatga
    34981 gagcgatctc tttttgcatt tcctgcgcta cgcgctgcgg gcgaccaaat tctttcgcca
    35041 taataaattc tcctgacaaa aaaggggctg ttagcccctt tttaaaatta atttcaggtg
    35101 gaagggctgt tcacgttggc ctgataagac gcgccagcgt cgcatcaggc aatccatgcc
    35161 ggatgcggcg taaacgcctt atcccgcatg gaaccctaaa aaccttaagc gatggtacgt
    35221 tggatttcga tgatttcgaa tacttcgatc acatcgccag tgcggacgtc gttgtagttc
    35281 ttaacgccga taccacattc cataccgtta cggacttcgt taacgtcatc tttgaagcgg
    35341 cgcagggact ccagctcgcc ttcgtagata accacgttgt cgcgcagaac gcggatcggg
    35401 ttgtgacgtt taaccacacc ttcggtaacc atacaacctg cgatggcacc aaatttcggt
    35461 gatttgaata cgtcacgaac ttccgccaga ccgataatct gttgtttcag ttccggagac
    35521 agcataccgc tcattgccgc tttcacttcg tcgatcaggt tatagatgac ggagtagtaa
    35581 cgcagatcca ggctttccgc ttcaatcact ttacgtgcag aggcatcagc acgtacgttg
    35641 aagccaacca ggatggcatt ggacgccgca gccagggtag cgtcggtttc ggtgatacca
    35701 cctacgccag aaccgatgat cttcacttta acttcgtcag tagacagttt cagcaaggag
    35761 tcggagatag cttccacaga accctgtacg tctgccttca ggacgatatt cacttcgtga
    35821 acttcgcctt cggtcatgtt ggcgaacatg ttctcgagtt tagatttctg ctgacgcgcc
    35881 agtttaactt cgcggaattt accctgacga tagagtgcaa cttcacgcgc tttcttctcg
    35941 tcacgtacaa cggtaacttc atcacccgca gccggaacgc cggacaggcc gaggatttcc
    36001 accggaatgg acggacccgc ttccagcact tcctgaccca gttcgttgcg catcgcacga
    36061 acacgaccgt attcgaagcc acacagaacg atatcgccct tgtgcagagt accttcacgt
    36121 accagaacgg tagcaaccgg accacgacct ttatcgagga aggattcgat aaccgcaccg
    36181 ctcgccatac ctttacgtac cgctttcagt tccagaactt ccgcctgcag caggatagcg
    36241 tccagcagct catcgatacc agtacccgct ttcgcagaca catgtacgaa ctggctttca
    36301 ccgccccact cttccggcag gatgccgtac tgggagagtt cgttcttaac gcgatccgga
    36361 tcggcttctg gtttatcgat cttgttcact gcaaccacca ccggaacctg cgccgctttc
    36421 gcgtgctgga ttgcttcgat ggtctgcggc attacgccgt cgtcggcagc aacaaccagg
    36481 actacgatgt ccgttgcctg cgcaccacga gcacgcattg aagtaaacgc ggcgtgcccc
    36541 ggggtgtcca ggaaggtgat catgccgttt tcagtttcaa cgtggtatgc accaatgtgc
    36601 tgggtaatgc cgcccgcttc gccagaggcc actttcgttg aacgaatgta gtccagcaga
    36661 gaggttttac cgtggtcaac gtgacccatg atggtcacaa ccggcgcgcg cggttcagcc
    36721 gctgcacccg tgtcacggtc gctcattacc gcttcttcca actcgttttc acgacgcagg
    36781 ataactttgt ggcccatctc ttcagcaacc agctgtgcgg tttcctgatc gataacctgg
    36841 ttgatggttg ccattgcgcc cagtttcatc atcgctttga tgacctgaga gcctttaacc
    36901 gccatcttgt tcgccagttc gccaacggtg atggtttcgc cgatcacaac gtcacggtta
    36961 acggcctgag caggcttctg gaagccttgc tgcagcgaag aacctttacg ttttccgccc
    37021 ttaccgccac gtactgctgc gcgtgcttct tcacgatcag cttttgattc agcgtgtttg
    37081 ttgcctttct tcggacgcgc agctttcgcg ttacgaccac ggccacggcc gccttcgact
    37141 tcacgatcgc tttcgtcttc tgcctggcga gcatgttgag aagtagtaac gtgataatcg
    37201 ctggaatctt cagtcggttc cgcgttatca gtccatttgt tttcttccgc catacgacgt
    37261 gcttcttcag caacgcgacg tgcttcttct tcgagtttac gacgcgcttc ttcttcggct
    37321 ttacgcttga gctctgcagc ttcctgctca cggcgggctt tttcagcctg ggcgttttta
    37381 gtcatatcgt cttgttgatt gctcactttg tctttttccg cagcttcacg tttcgcttgt
    37441 tcagcagctt cacgcttagc ttgttctgcg gcctcacgtt cagctttttg ttgcgcctca
    37501 cgtttggccg attcttctgc ctcacgacgg gcttgctctt ccgcttcacg ctgcgcttgc
    37561 tcttccgcag caaggcgttc agcctcttgc ggatcgcgtt tcacaaaggt gcgtttcttg
    37621 cggacttcga tttgtaccga tttgcttttt ccaccggtac caggaatgtt aagggtgctg
    37681 cgtgttttac gttgcagcgt caatttgtcc gggcctgaat ttttctgatt caggtggtca
    37741 atcaaagtct gtttctcttg tgcagacaca gagtcgtcag cagacttccg gatacctgca
    37801 tcagcaaatt gctgtaccag gcgttccacg gaggtctgtc gctctgcggc cagcgtttta
    37861 atcgttacat ctgtcatgct gttccttcct gctacagttt attacgcttc gtcaccgaac
    37921 cagcaaatat tacgggcagc cataatcagt gctccggctt tttcgtcggt caacccttcg
    37981 atatcagcca gatcatcaat gccctgttcg gcgagatctt ccagcgtaca aacgccacgg
    38041 gcggccagtt tgaatgccaa atcacgatct atcccttcaa ggttcagcag atcgtcagcc
    38101 ggtttgttat caccgaggct ttcttcctgg gcctgtgcaa tggtggccag tgcattttta
    38161 gcacgctcgc gcagtgcttc aacggtcggc tcatcaaggc cttcgatttc caacagctct
    38221 ttcatcggca cataggccag ttcttccagc gtcgagaagc cttcttctac cagaacagtc
    38281 gcgaagtctt cgtcgatgtc gagatatttg gtgaaggtgt cgatcgctgc gtgcgcttcc
    38341 gcctgatgct tagcctgcag gtcgtcaacg gtcatcacgt tgagttccca accgctcagc
    38401 tgcgaagcca gacgcacgtt ctgaccatta cggccaatcg cctgcgccag gttaccggct
    38461 tcaacggcga tatccatggt gtgtttatct tcatccacca cgatagaagc aacgtctgcc
    38521 ggtgccattg cgttaatcac gaactgcgcc gggttatcat cccacaggac gatatcgata
    38581 cgctcgccgc ccagttcagt agaaaccgcc tgaacacgcg cgccacgcat acctacgcaa
    38641 gcacctaccg gatcgatacg tttatcgttg gttttcaccg cgattttcgc acgagaaccc
    38701 ggatcgcgag ccgctgcttt aatttcaatc acttcttcgc cgatttccgg cacttcaatg
    38761 cggaacagtt cgatcagcat ttccggcttg gaacgggtga cgaacagttg cgcgccacgc
    38821 gcttccgggc gaacggaata gagcacgcca cgaacgcggt cgccaggacg gaagttttca
    38881 cgcggcagca tatcttcgcg caggatcacg gcttcagcgt tgttgcccag atccagagag
    38941 atgttgtcgc ggtttacttt tttcaccacg ccggtgatga tttcaccttc gtgttcacgg
    39001 aactgatcaa ccaccatcgc acgttcggct tcacgcactt tctgcacgat aacctgtttt
    39061 gccgtctggg tggtgatacg gtcaaaggta acagactcaa tctgatcttc aacgtaatcg
    39121 cccaggttca ggctttcatc ttcataacgt gcggcttcaa gggtgatttc cttggtcggc
    39181 tgggtgactt catcaacaac taaccagcga cggaaggtgt caaaatcacc gcttttgcga
    39241 tcgatctgta cacggacgtc gatctcttgc tcatattttt tctttgttgc tgtcgccagc
    39301 gcgctttcca atgcttcgaa aatcttctcg cgaggtagcg ccttttcatt ggatacggct
    39361 tcaactacag ccaaaatttc tttgttcatc gcgggctttt cacctcatcc agactattaa
    39421 aagtggggaa ccaggttcgc cttctggata ttactcagcg cgaacacttc atctttacct
    39481 tcgacggtaa ctgtgatcat ttcaccgtct accgctttga taacgccctg ccatttacga
    39541 cggttttgta ccgccatgcg gagaaccaga gtcacctctt ctccgacgaa acgggcgtag
    39601 tgttcagccg tgaacagtgg gcgatcgaga cccggtgagg agacttccag gttataagca
    39661 acggtgatgg gatcttcaac atccagcaca gcacttacct ggtggctcac atcagcacaa
    39721 tcatcaacat tgatgccatc ttcactatca atatagatgc gcagtgtgga tgtgcgaccg
    39781 cgaataaatt cgatgccaac cagttcaaaa cccagggcct caactggcgc agtaatcatc
    39841 tctgttaatt tttgctctaa tgtggacaag cccaccccca agacataaaa aaagggccta
    39901 aagcccagtt attctgtagt cagataacaa aaaaccccga taaatcgggg ctttatataa
    39961 ctgaacccta taaccgcaac tgcggtctgg agcactttcc agaaggattt tttcaaatcc
    40021 cactacgaag gccgaagtct tcacagtata tttgaaaaag gactctaagg gaaagtggtt
    40081 gcgggggccg gatttgaacc gacgaccttc gggttatgag cccgacgagc taccaggctg
    40141 ctccaccccg cgcctgaaac gtggcaaatt ctactcgttt tgggtaaaaa atgcaaatac
    40201 tgctgggatt tggtgtaccg agacgggacg taaaatcttc aggcattata gtgatccacg
    40261 ccacattttg tcaacgttta ttgctaatca cgtgaatgaa tatccagttc actttcattt
    40321 gttgaatact tttgccttct cctgctctcc cttaagcgca ttattttaca aaaaacacac
    40381 taaactcttc ctgtctccga taaaagatga ttaaatgaaa actcatttat tttgcataaa
    40441 aattcagtga gagcggaaat ccaggctcct catcagttaa ttaagcaggg tgttatttta
    40501 tgacgacgat tctcaagcat ctcccggtag gtcaacgtat tggtatcgct ttttccggcg
    40561 gtctggacac cagtgccgca ctgctgtgga tgcgacaaaa gggagctgtt ccttatgcat
    40621 atactgcaaa tctgggccag ccagacgaag aggattatga tgcgatccct cgtcgtgcca
    40681 tggaatacgg cgcggagaac gcacgtctga tcgactgccg caaacaactg gtggccgaag
    40741 gtattgccgc tattcagtgt ggcgcatttc ataacaccac tggcggcctg acctatttca
    40801 acacgacgcc gctgggccgc gccgtgactg gcaccatgct ggttgctgct atgaaagaag
    40861 atggcgtgaa tatctggggt gacggtagca cctataaagg aaacgatatc gaacgtttct
    40921 atcgttacgg tctgctgacc aatgctgaac tgcagattta caaaccgtgg cttgatactg
    40981 actttattga tgaactgggc ggtcgtcatg aaatgtctga atttatgatt gcctgcggtt
    41041 tcgactacaa aatgtctgtc gaaaaagcct actccacgga ctccaacatg ctcggcgcaa
    41101 cgcatgaagc gaaggatctg gaatacctca actccagcgt caaaatcgtc aacccgatta
    41161 tgggcgtgaa gttctgggat gagagcgtga agatcccggc agaagaagtc acagtacgct
    41221 ttgagcaagg tcatccggtg gcgctgaacg gtaaaacctt tagcgacgac gtagaaatga
    41281 tgctggaagc taaccgcatc ggcggtcgtc acggcctggg catgagcgac cagattgaaa
    41341 accgtatcat cgaagcaaaa agccgcggca tttatgaagc ccctgggatg gcactgctgc
    41401 acattgcgta tgaacgtttg ttgaccggta ttcacaacga agacaccatt gagcagtatc
    41461 acgcgcatgg tcgtcagttg ggccgtctgc tgtaccaggg acgttggttt gactcccagg
    41521 cgctgatgct gcgggactct ctgcaacgct gggttgccag ccagatcact ggtgaagtta
    41581 ctctggagct gcgccgcggg aacgattatt caatcctgaa taccgtctca gagaacctga
    41641 cctacaagcc agagcgtctg acgatggaaa aaggcgactc ggtgttctcg ccagatgatc
    41701 gtatcggtca attgaccatg cgtaacctgg atatcaccga tacccgcgag aaacttttcg
    41761 gttatgccaa aactggcctc ctctcctcct ctgccgcttc aggcgtgccg caggtggaga
    41821 atctggaaaa taaaggccag taattcgaca tcaaccctgc ccttcagggg tggggttacc
    41881 ttgcttatcc tctgtaaact cctttctgag catcacataa tcgaaaacat catggtttgc
    41941 atccacaacc cgtgagtctc ctttatagtg tgcaatcact tcctccagcg ttttcggttg
    42001 ttccggcttt gttaccccca tccacgcatt aatcaggtaa ttattgtaag ctgtggaaaa
    42061 gatgttgttt tccgttgtgc gatccacgcc atccccaaga acggggctat accagataaa
    42121 catcgggaca tgatatgcct gctggctggc ctccctgcct ccatgaaaat agacgttctt
    42181 cttcgtaggg tcacgttcca ggccgtggtc ggcaaaatac atgaccgagg cgcggcgatc
    42241 ttttaataat tcaaaaacct gacctagcaa actatctgtg taatgaatgg agttgtcata
    42301 gcaggcatcc tgatcgtcct gcggttgaaa cacggcgctg gattgcggat aggcgctaca
    42361 agccggttca tggcttccat ttaaatgaag aacaatcagt ttcttttgct gcgtattttg
    42421 ctgtaatgct tgcgataaat gcggcaacaa caattcatca aatcctctga cataaactgt
    42481 ttccatggcg cgcatggcga tactggtaac tgctgtacca ttctgccgaa aagcggattg
    42541 cgagcttagc cagaaagtct gaaatcctgc ttgattagcc atattaataa tgttgtccgg
    42601 gtaattatga atgtcatgac tcaaaacgga atcagcagtt aaagaaaggg gaaccgacag
    42661 cgcagtgtaa ggtgcaccgc ttattgcttg attaaacagt ttgatctgtt ttctttgtgc
    42721 ttcgacttgc ggtgtcgtag agcgtgtata tccatacaaa gacatattgt cgacgcgtac
    42781 agactccccc acaatcaaca cgtaggtatc aatacctgta tccctgactg ataattgaaa
    42841 atacggcacc gtgtttgcaa ttgagagtaa tctttgatgc tctttcgctg ctaaagcaaa
    42901 ataattgaga ttgaaaaacg gtgtataggt agcaaatcgc gacgctagta tatatggact
    42961 gaacgctttt ttattttttg catctttata agcaaattga caagcggaaa ataaactgcc
    43021 cgaaatgaca atcagcaata atattcctgt cacttttttc gtcggcaagg aaacatcata
    43081 ttttattatt actgccaaaa aaagaaggga taaaaaggca aaagcacata gataagggat
    43141 atacatcccc agcattttga caacttcatc cggatcactc tgcagcacac taatcgcaaa
    43201 tccatcattg aaagttgtac caaaggtaca ccatgaatag aggctaatac tcatgtctgc
    43261 cgctgtcagt aacacaaatg gtgcggcaac aagcaggcga agcggaaggg atgatatacg
    43321 tttaacaacc aggatggtca gaacgaagaa cagcaatcgc tgaaccatat gcgctccgga
    43381 agaggcgact aagttcgtaa taccaaaaac aataacacaa agatacaaca accaatgaga
    43441 tttaaaactt ctaacaaatt tgttgaatac tgtcatccag aaatccaagt tcacaaccaa
    43501 tataaacaat tatcaacaat gttcaatttt atatcagaca tgtttttcaa ctctgatcca
    43561 tatcagaccg aaacactgtt agttttttct ttttaaatga tgcggccatt gttctcagta
    43621 attaaataac attatcaatg cgtctctaaa cattacgcag aacgtagcca tcagtagtta
    43681 tactgacagg agcaggatgg tttatcgaga agtaaacata tccgtattcg taccggaact
    43741 ggagttgaca caataaagtg ccaattatgt cagcagaagg gaaaagttta taactaaggc
    43801 gtaccgatac catcgtttca aagtaccagc tacgagtaaa gcaactggac gagatacaga
    43861 tacctgacaa ccattcctca gaccaggacc aaaacgaaaa aagacgcttt tcagcgtctc
    43921 ttttctggaa tttggtaccg aggacgggac ttgaacccgt aagccctatt gggcactacc
    43981 acctcaaggt agcgtgtcta ccaattccac cacctcggca cggatactac tttttagttc
    44041 gggatatcgc tggtcggctt agccggagct gctggctgaa tttgttcggt tttcgccggc
    44101 gcactcaggt tttcccattc gctaccttta ttggttttgt tgctattgat gttacccagc
    44161 accagactga tgatgaagaa taacgttgcc agcagcgctg tcatgcgggt catgaagtta
    44221 ccagaaccac ttgaaccaaa cagcgtagcg gaagcgcctg ctccgaagga ggctcccata
    44281 tcagcgcctt taccttgctg cagcatgatc agaccaacaa ggccaattgc cacaataagg
    44341 aaaactacta aaagagcttc gtacataatc aacctgttcc ttgcggagtt gccgcgtacc
    44401 aatgcttcaa ccaataaagc gggagttttt aatctttccc actgaagcgg gtgtgaatac
    44461 taaccaaagc gaatgacctt cgcaagggca attttattgc attgtatcaa ctgcggaaaa
    44521 aaacagcaaa accgatgtgt tcgctgagaa aaaaggcgac aatcgccgcc tttttagcca
    44581 gttatttatc gttttaaacg gcttttactg catcggcgat gcggtgtgca aattcagtca
    44641 cctgcgcttc gtcttcgcct tccaccatca cgcgaattaa cggttcggtg ccggatttac
    44701 gcaacagcac acgtccacgg ctacccagcg cagcttcaac ttctgcggtc acagctttga
    44761 ctgactcatg ctccagcgga tcgccgctgc ctgctgtgta acgcacgtta accagaatct
    44821 gcgggaacat tttcatgccg ctgcaaaggt cgtgcaggct catatggtta cgcgccatcg
    44881 ccgccagcac ctgcaagcca gcaacaatgc cgtcaccggt agtggtttta tccagcagga
    44941 tcacatgacc ggagttctct gcaccgatac gccagccttt ctcctgcatt ttttccagta
    45001 cgtagcggtc acccactttc gcgcgcgcaa atggaatacc taactgtttc agcgccagtt
    45061 caagccccat gttgctcatc aatgtaccca cagcaccacc acgcagctgg ccctgacgaa
    45121 gaccttcacg cgcgatgata tacatgatct gatcgccatc gactttattg ccttcatggt
    45181 caaccataat cacgcgatcg ccgtcgccgt cgaaggcaat accgagatcc gctttttcag
    45241 ccagcacacg agcctggagc gcgcgaacgt cggtagcccc cacttcggca ttgatgttta
    45301 caccgttcgg ctcacaaccg atagcgataa cgttcgcccc cagttcgcgc agcacgttcg
    45361 gcgcgatgtg ataagtcgca ccgtttgcac aatccaccac aatcttcagt tcactgaggc
    45421 taagttcgtt cgggaacgtg gctttgcaaa actcgatata gcgacccgca gcatcaacga
    45481 tacggctggc tttacccagt tctgccgaat caacgcagct gatctctttt tccatttccg
    45541 cttcgatggc ctcttctacc gcatccggta gtttggtgcc gtcgatagag aagaatttaa
    45601 tgccgttatc gtagaatggg ttatgcgatg cagaaattac aattccagcc tctgcgcgga
    45661 aggtacgcgt cagataagcc acggccggtg ttggcatcgg gccagtgaag agcgcggaaa
    45721 gaccggctgc cgcaagcccc gcttccagtg ctgactccag catatagcca gaaatacgcg
    45781 tgtctttacc aataataatc ttacgggagc cgtggcgcgc cagcacttta cccgcggccc
    45841 aacccagctt aagcacaaaa tcaggggtga tcggcgcatc ccctacacga ccacgaatcc
    45901 catcggtacc gaaatattta cgattactca tagcgtttgt tttcctttgc agacagagtg
    45961 gcttccacca cccgcatcgc ttctacggtt tctttgacgt catgaacacg aatgatgtgc
    46021 gcgccttgca ttgcggcaat gaccgcacag gccagactac cgctcaaacg ctcggacggc
    46081 cccacgttca gcagctgtcc aatcatcgat tttcgtgaca tacccaccaa cagcggcagg
    46141 ttgaaatggt gaaattcagc caggcgcgcc agtaatgaat agttatggga gagattttta
    46201 ccgaaaccga atccggggtc gagcaacaat ttctcttttg cgatacccgc ctgctcgcaa
    46261 cgtgctattt gctcaataaa gtagcgattc acttctgcaa agacatcgtg atacttcggg
    46321 gcttcctgca tggtttttgg atttccctgc atatgcatca gacaaaccgg taaaccggtt
    46381 tctgcagccg cctccagagc gccaggttcg gaaagggagc ggatatcgtt aataatgtga
    46441 gcgccaactt tcgctgactc acggatgact tctggtttgg atgtatctac cgagatccag
    46501 acttcgaagc gttgagcaat tgcctcaacc acaggaataa cacgttgtaa ctcttcttca
    46561 acgctaactt ccgccgcccc tggacgtgtg gactcgccac caacgtcaat gatcgtcgcg
    46621 ccagcgttga tcatcagatt cgcatgtttc accgcatcta tcagcgagtt atgcgtgcca
    46681 ccatccgaaa aggaatcagg cgtgacgttg aggatcccca ttacgtgagg atggctaagg
    46741 tccagtgaag taccctgggc aaagagtttc ataatgttat ccctggtatg aattgataag
    46801 aaaaaacccc ggagcacgcc ccggggtttt tggtacaaat acagtcatct gatgcgggaa
    46861 cttacttgtc gcctaactgc tctgacatgg tgttacccgg gttcggcgta cgcggttcat
    46921 caaccggacg aggagccttt ggactaccat tgtcgccaga attgttagaa gcgcctggtt
    46981 cttcccagcc cgctggcgga cgtacatcgc gacgtgccat caggtcatca atctgcggtg
    47041 cgtcgatagt ctcatatttc atgagagcat ctttcatcgc atgcagtata tccatattgt
    47101 cggtcagaag ctgacgcgca cgattatagt tgcgctcaat cagtgctttc acttcctggt
    47161 cgatgatacg tgcagtttca tcggacatat gtttcgcctt cgctacgcta cggccgagga
    47221 acacttcacc ttcttcttcc gcgtacagca gtggacccaa tttctcagag aagccccact
    47281 gagtcaccat gttacgtgcc aggttggtcg caactttaat atcgttggac gcaccggtag
    47341 aaacatgttc cggcccgtag atgatctctt ctgccagacg accaccgtac agcgtagaaa
    47401 tctggctttc cagtttctga cggctagcgc tgattgcgtc gccctcaggc aagaagaaag
    47461 tcacacccag cgcacgaccg cgtgggataa tcgtcacttt gtgcaccgga tcatgttccg
    47521 gcaccaggcg accgataatc gcatgacccg cttcgtggta agccgtcgat tctttctgcg
    47581 cttccgtcat caccatggag cgacgttccg cacccatcat gattttgtct ttcgctttct
    47641 cgaactcaac catcgacaca acgcgtttgt tgccacgagc agcgaacagt gccgcttcgt
    47701 tcaccaggtt cgccaggtca gcaccggaga aaccaggagt accacgggca atgattgccg
    47761 cgtcgatatc gggtgccaat ggtacgcgac gcatgtgaac tttcaggatc tgttcacgac
    47821 cgcgaacatc tggcaagcca accacaacct gacggtcgaa acggccagga cgcagcagcg
    47881 ccgggtcgag aacgtccgga cggttagtcg cggcgataac gatgatacct tcgttacctt
    47941 cgaagccatc catctcaacc agcatctggt tcagagtctg ttcacgttca tcgtgaccac
    48001 cgcccagacc tgcgccacgc tggcggccta cggcgtcgat ttcatcgata aagatgatgc
    48061 acggtgccgc tttcttcgcc tgttcgaaca tgtcacgaac acgggatgca cccacaccga
    48121 cgaacatttc tacgaagtca gaaccggaga tagtaaagaa cggaactttc gcttcgcctg
    48181 caatcgcttt cgccagcaac gttttaccgg tacccggagg accgaccatc aggacgcctt
    48241 tcgggatctt accgccgagt ttctggaagc ggctcggctc gcgcagatac tcaaccagtt
    48301 cagcaacttc ttcttttgct tcgtcgcagc ccgcaacgtc agcaaaggtc gttttgatct
    48361 gatcttcagt cagcatgcgc gctttgctct taccaaacga catggcacct ttgccaccgc
    48421 cgccctgcat ttgacgcatg aagaagatcc agacaccaat cagcaacagc atcgggaacc
    48481 aggagatgaa gatagaagcc agcaggcttg gttcttcagg cggttcaccg acaaccttga
    48541 cgttcttggt caacaagtta tccagtaatt tcggatcctg aaccggaatg taagtggtat
    48601 aacggttact atctttcttg gtaacgttga tttcacgtcc gttgatacgc gcttcacgaa
    48661 cctggtcgtt attgacctct tgtaggaagg tagagtaatc caccttacgg ccattagact
    48721 cgctgggccc aaagctctgg aatactgaca tcagcacaac ggcaatgacc agccagagta
    48781 ttaggttttt cgccatgtca ctcaagggat taacctctta ttacaactgt gttaaaaaca
    48841 gcgtcaggat actctatatc cagcatcttt caaactttcg tctgaaatct cccggttagg
    48901 gtttacgccc ggtcgctaca atatacactt cccgcgaacg tgcgcgagaa gagtccggct
    48961 tacgaacttt gaccttcgta aacagggagc gaatttccct tagatactca tcgaaacctt
    49021 cgccctggaa caccttcact acaaaactgc cacctggcgc taatacatca cgacacattt
    49081 ctagcgccag ttccaccaga tacatggcac gggggatatc caccgccggt gttccgctca
    49141 tgtttggtgc catatcggac atgacaacct ggactttgct gtcgccaacg cgctccagca
    49201 gtgctttcat caccagttca tcacgaaaat cgccctgaag aaagtccaca ccaacgatag
    49261 gatccatagg taaaagatcg caagcgatga tacggccttt accgccaatt tgggtgacca
    49321 catattgtga ccaaccaccc ggcgcagcac caaggtcgac aaccgtcatt cccggtttaa
    49381 agagtttgtc actttgctgt atttcatcaa gtttaaacca ggcacgggaa cgtaacccct
    49441 ttttctgtgc ctgttgaaca tatttatcgc taaagtgttc ctgaagccag cggctggagc
    49501 tggcagaacg cttcttacct gtcatttaac tttcccattg ggataactca tcgtaaccaa
    49561 ttgcgtaaat ttttacgcgc ctatttggcg atatatggga gatggcggta gaatgacccg
    49621 ttttcaatcc caacgtaagc aaaaaatacg atgaatctga gtactaaaca aaaacagcac
    49681 ctgaaaggtc tggcacatcc gctcaagcca gttgttctgc ttggcagtaa tggtttgacc
    49741 gaaggggtgc tggccgagat tgaacaagcg ttagagcacc atgaactcat caaggtgaaa
    49801 atcgccaccg aagatcgcga aactaaaacc ttgatcgtgg aagctatcgt gcgcgaaacc
    49861 ggcgcctgta atgtacaggt catcggtaaa acgctggtgc tttatcgccc aactaaagaa
    49921 cgtaaaatct cgctgccacg ctaagattat cctaaagtta cacacatttg ctgtgtaaaa
    49981 cgaggggttt tccgcaggca ggagagcaaa atgccacgct ctgttcgttg ataaaaggcc
    50041 gcatagcggc ctttttcctt tctttacaat acatcaacat cttgagtatt gggtaattct
    50101 tacaggtatt ccaccttaat tacttcaaat tctacttcgc cgcccggcgt tttgatgacc
    50161 acaacatcat cttcttcttt gccgatcagg ccacgagcaa taggcgagtt tacagaaatc
    50221 aggttttgtt taaagtcagc ttcgtcatcg ccaacgatgc gataagtctg ttcttcgtca
    50281 gaatccagat tcagcaccgt tacggtagca ccaaaaataa cgcgcccatt gttgggcatt
    50341 ttggtgacat caatcacctg cgcgttcgac agcttggctt cgatgtcttt gatacggcct
    50401 tcgcagaagc cctgctgttc acgagctgcg tggtattcgg cgttttcttt caggtcgcca
    50461 tgctcacgcg cttccgcgat agcagcaatg atttcaggac ggcgcacaga tttcagaaaa
    50521 tccagctctt cgcgtaattt ttcagcgccg cgtaaggtca tcggaatagc ttgcatttgt
    50581 tatacctctt gaatattcct gatggggcaa gtcttcaccc accccggctg ttcggcctgc
    50641 ccggcataac tcctcaccgg gaccagaagc aaaaaaatac cgacccgggt acaagtccca
    50701 ggtcagctac aattcacatt ttgatagtca ttttaccctg aagttcccga agggtcatcg
    50761 tttactttat agggcgttgc gccgtagtat gacggctcga ttccaggttg ttagcgcgag
    50821 attatgcgat tttccagatt tatcatcgga ttgaccagct gtatagcgtt cagtgttcag
    50881 gccgcaaatg ttgatgagta cattactcaa ctccccgctg gtgccaacct tgccctgatg
    50941 gtgcaaaaag tcggcgcgtc ggcccccgct attgattacc acagtcagca gatggcgctg
    51001 cccgccagta cgcagaaagt gattactgca ctggcggcgt tgattcaact cggccccgat
    51061 tttcgtttta ccacgacgct tgaaaccaaa ggcaatgtgg aaaacggcgt acttaagggt
    51121 gacttagtgg cgcgatttgg tgccgatccg acgttaagac gtcaggatat tcgcaatatg
    51181 gtcgcgactt tgaaaaaatc tggcgtcaac caaatcgatg gcaatgtgtt gatagatacc
    51241 tccattttcg ccagccacga taaagccccc ggctggccat ggaatgacat gacacaatgc
    51301 tttagcgctc cgcctgccgc cgccatagtt gaccgcaact gtttctccgt ctcgctctat
    51361 agtgccccaa aacctggtga tatggctttt atacgcgtgg catcttatta ccccgttacg
    51421 atgttcagcc aggtacgcac cctcccccgt ggttctgccg aagcgcaata ctgcgagctg
    51481 gatgtggtgc caggcgacct gaaccgcttt acgctgaccg gatgcctgcc acaacgttct
    51541 gagccgctcc cgttggcttt tgccgtgcag gatggtgcca gctatgctgg agcaattctg
    51601 aaagatgagt taaaacaggc aggtatcacc tggagcggaa cactgctgcg tcagacgcag
    51661 gttaacgaac ccggaacggt agttgccagt aaacagtcgg ccccgttgca cgatctgctt
    51721 aagattatgc tgaaaaagtc ggacaacatg attgccgata cggttttccg catgatcggc
    51781 catgcgcgct tcaatgtgcc tggaacatgg cgggccgggt cggacgccgt gcgtcagatc
    51841 ctgcgccagc aagccggtgt cgatattgga aataccatta ttgccgatgg ttcagggctt
    51901 tcgcggcata acctgattgc tcccgccacc atgatgcagg tgctgcaata cattgcccaa
    51961 cacgacaatg aacttaactt tatctctatg ctgccgctgg cgggctatga cggctctttg
    52021 cagtaccgtg caggtctgca tcaggcgggc gtggatggaa aagtctcagc gaaaaccggt
    52081 tcgttgcagg gggtatataa cctggcggga ttcattacca cagcgagcgg gcaacgaatg
    52141 gcgtttgtgc aatatctttc tggctatgca gtagaaccgg cggatcagcg taatcgccgt
    52201 attccgttgg tacgttttga aagccgtttg tataaggata tttaccagaa caattagtct
    52261 aaagagaaac cccggcacat ggccggggtt ttgctgatta acgcttgtaa atgaactcaa
    52321 cgccttcttc gtcgtcttcg tcccagtcgt catcccagtc ttcatcatcc tcttcagcaa
    52381 tctcttcaag ctgctggcga tgataatcat cccacatgaa ttcgactttc tctggctgtt
    52441 tcgcttcttc agcctgcacg accgggtttt caatgataaa ggtcatcaca tcccagcaga
    52501 gatctttcac gcccagtccg ctcgccgcag agatcagata atatttatct tcccaaccca
    52561 gcgcctcagc gatcgctttc gctttctctt cggcttctgc cttatccagc aggtcgatct
    52621 tgttgaagac taaccaacgc ggtttcgccg ccagatcctg gctgtatttt tccagctcgc
    52681 tgataataat acgcgcgttt tcaaccggat cggtgccatc aatcggatcg atatcgatga
    52741 ggtgcaacag gacgcggcaa cgctccaggt gcttcaggaa gcgaatgccc agacctgcgc
    52801 cttctgcagc gccttcaatc agtcctggaa tatcggcaac aacgaagctc ttttcgttgt
    52861 ccattcgtac cacaccaaga cttggcacca gagtggtaaa cggataatcc gccactttcg
    52921 gtttagccgc cgatactgcg cggataaatg tcgatttacc cgcgtttggc atccccagca
    52981 taccgacgtc agccagcagc atcagctcca gtagcagctc acgcttatcg cccggcgtac
    53041 cgttagtttt ctgccgtggt gtacggttaa ctgacgattt gaaacgggta ttgcccagac
    53101 cgtgccagcc gcccttagca accagcagac gctgaccgtg tttggtcata tcgcccatgg
    53161 tttcaccggt accctggtcg ataacacgcg tacctaccgg cactttaatc gtcacgtctt
    53221 taccgcgctt accggtacag tcgcggcttg cgccattctg accacgctct gcgcggaaag
    53281 atttttcaaa acgataatcg ataagcgtgt tcaggttctc gtcggcttcc atccatacgt
    53341 caccgccgtc accgccgtcg ccgccatccg ggccgccttt cggaatatac ttttcgcggc
    53401 ggaagctcac gcaaccatta ccgccatcac ctgcaacgac cagaatcgat gcttcatcaa
    53461 caaacttcat tttattctcc gtaaatcatt cgcctgcgcg gggttgcgat accaccgttg
    53521 tatgcttacg taatccgccc caaatacgat gaccaatggc ggaatacatc gcgcccgcaa
    53581 ccacgacaaa cgcaccgaga taacctaaaa ggtttaacat cggtctggcg aagaaatcgg
    53641 gccaggccag tgataaaaga tctgaaaaaa acagcgtaaa cagtggggtg agcgtaatga
    53701 tcgcgctcac ctgcgctgcc tgccagcgag ccatcgcttc cgccagggcg ccatatccta
    53761 ccaatgtatt cagtccgcaa aaaattaaac atgcgagctg ccagtggcta agctgcgcta
    53821 tcactccagg ctttgccaga gggaagagcg caattgtaca taaagtgtac agcaaaaaca
    53881 ggatctgcgg tgaggccagt cgacgcaata aaaccttttg cgccacgcca taactcaccc
    53941 acaccgtcgc cgcaccgacc ccaaagatta ctccccaggt gtaatcggtg agctttgtaa
    54001 atatctcgac cagactggtg ttaaaaaaca tcaccaggcc gctcaggagc atcaatgccc
    54061 ctacaacctg agtgctgcgc attttctctt tcaggataaa tacgctggca accatcatgc
    54121 caactggcga gagttgccca atcacctgcg aagcggtcgg acccaggtat tgcaaggatg
    54181 agctgaacag gatgaagttc ccaaacagcc cggcggtcgc cactgccaac aaaatcaacc
    54241 agcgtggctt acgaaacacg cgtaatggcg gcaaccgctt cttcaccgca agaatggcac
    54301 ccaggccaat actcgccatc aagaaacggt aaaacacgat tgtcggaggt tccatcacct
    54361 ccagcacctg cttcattgcg attggcaacg ccccccagca aattgctgtg gtgagcgcca
    54421 aaagaatgcc aatgcctgcc tgctgcttca taccgtattc cctgcaaagc gcatttgcgg
    54481 attaacgtcg caccatgcgc atttttaccg gttatcgaat gtaaaaagcc ccgcaacgtg
    54541 ttgcggggct ttcatccgtt accgggacgc gaaaaactta ttcagcttcg atgctgataa
    54601 atttacggtt tttcgggcct ttaacttcga atttcacttt accgtctgct ttagcaaaca
    54661 gagtgtggtc acgaccgcaa cctacgttag cgccagcgtg gaatttggta ccacgttgac
    54721 gaacgatgat gctacccgcc agaacggatt cgccaccgaa acgcttaacg cccaggcgtt
    54781 tagcttctga atcgcgaccg ttacgtgtgg agccgccagc ctttttatgt gccatttgaa
    54841 atctctcctc aggtcttagg cgctgatgcc agtaattttc acatcagtga accactgacg
    54901 atggccctgc tgcttacgat agtgtttacg acgacgaaac ttaacgattt taactttctc
    54961 gccacgaccg tgagcaacaa cttcagcttt gattacgccg ccatcaacga aaggaacgcc
    55021 gattttgact tcttcaccgt ttgcgatcat cagcacttca gcgaactcaa cagtttcgcc
    55081 agttgcgatg tccagctttt ccaggcgaac ggtctgacct tcgcttactc ggtgttgttt
    55141 accaccactt tggaaaaccg cgtacatata aaactccgct tccgcgcaca ccttttcaat
    55201 gattcagagt gcgctataaa tattcacaat agggcgcgaa tattacgcaa aacgcacgcc
    55261 tttgacaagt gctacagtca atacacgaag aaaaaaaaca caacttgtac ggtaacgttt
    55321 atctgtgcca ttttttcagt acaatcaccc tatattccta accataaacc ctaagttgcc
    55381 tttgttcaca gtaaggtaat cggggcgaaa agcccggctt ttgcgatgaa tttagaaaaa
    55441 atcaatgagt taaccgcgca agatatggcg ggtgttaatg cggcaatcct tgagcagctt
    55501 aattccgacg tccaactgat caatcagtta ggctattaca tcgtcagcgg cggcggtaaa
    55561 cgtattcgtc cgatgattgc tgtactggct gcacgagctg ttggctatga gggaaatgcg
    55621 catgtcacca ttgctgccct gatcgagttt atccacacgg cgactctgct gcacgacgac
    55681 gttgtggatg aatcagatat gcgcaggggt aaagctaccg ccaacgctgc atttggcaat
    55741 gccgccagcg tgctggtagg cgattttatt tatacccgcg cgttccagat gatgaccagc
    55801 ctcggctcgc tcaaagtgct ggaagtcatg tcagaagccg taaacgtcat cgcagaaggt
    55861 gaagttctgc aactgatgaa cgttaacgat ccggacatca ctgaagaaaa ctacatgcgc
    55921 gttatctata gcaaaaccgc gcgtctgttt gaggctgccg cgcagtgttc cgggattctg
    55981 gctggctgta cgccggagga ggagaaaggc ctgcaggatt atgggcgcta tctcggcact
    56041 gctttccagt tgatcgacga tttactcgat tacaatgccg atggcgaaca gttaggtaaa
    56101 aacgtcggcg acgatctgaa cgaaggtaaa ccgacgctgc cgctgctgca tgcaatgcat
    56161 catggcacac cagaacaggc acagatgatt cgtaccgcca tcgaacaggg taacggtcgc
    56221 catcttctgg aaccggttct ggaagcaatg aacgcttgtg gatctcttga atggacgcgt
    56281 cagcgtgccg aggaagaagc agacaaagcc atcgcagcgt tacaggtgct cccggacacc
    56341 ccgtggcgag aagcactcat cggcctcgcg cacatcgctg ttcaacgcga tcgttaatcc
    56401 cctcccctca tcccgcgcag cgcgcgggat gattccagta aattccataa aaacacttat
    56461 tcagctctat aacacctgaa aaatagggac gtacatcttt atatattctg aatattcaca
    56521 ctctttacag gaacttttta gagcaatagg ccatcaggag tatagtgatg ctcgacagaa
    56581 gaagtgttct gaatgaaagc gactaactta aggagtgagg aaagatggaa agtaatttca
    56641 ttgactggca tcccgctgac atcattgcgg gtttgcgcaa gaagggaacg tcaatggcgg
    56701 cggaatctcg cagaaatggt ttgagttcct caacgctggc gaacgcatta tcgcgcccct
    56761 ggccgaaagg agagatgatt attgcgaaag ccctgggaac tgacccctgg attatctggc
    56821 catctcgcta ccatgatccg cagacccatg agtttatcga cagaacgcag ttgatgcgta
    56881 gctacactaa accgaaaaaa tgagtggtct ggcggtagcc ccgcgaacgg ggctgccagc
    56941 tctcagacaa ttactcgcct ttcacacgct caatatttgc acctaaagcg cgcagtttgt
    57001 cttcaatgcg ttcgtagcca cgatcgatgt gataaatacg atcaactacc gtcgtccctt
    57061 ccgcaataca gccagccagc accaggcttg ctgatgcacg cagatcggtt gccataacct
    57121 gtgcgccaga aagtttttca acaccgtgac aaataacggt attgctttcg atttcggcgt
    57181 gcgcgcccat acggctcagc tctggcacat gcataaagcg gttttcaaag accgtttcgg
    57241 tgatgaagcc ggtcccttct gccaccaggt tcaacagcgt gaactgggcc tgcatatcgg
    57301 tcgggaatgc cggatgcggc gcggtacgta cgttaacagc cttcggacgt ttgccatgca
    57361 tatccaggct aatccagtct tcgccgactt cgatgtctgc tccagcgtca cgcagtttcg
    57421 ccagcacggc gtcgagagta tctggctgcg cgttacggca gataattttg ccgcgagaga
    57481 tcgccgccgc caccaggaaa gtaccggttt cgatacgatc cggcagcacg cgatagacac
    57541 cgccgcctaa acgttccaca ccttcgatga cgatacgatc ggtgccctga ccgctaattt
    57601 tcgcacccag cgtaatcagg aagttcgcgg tatcgacgat ttccggttca cgcgctgcgt
    57661 tttcaataat cgtggtgcct tccgccaggg ttgcagcaca catgatggtc accgttgcgc
    57721 caacgctgac tttatccatc acgatatgtg cgcctttcag acgaccatcg acggaagctt
    57781 taacgtaacc ttcttccagt ttgatggtcg cgcctaattg ttcgagacca gaaatgtgta
    57841 gatcaaccgg acgtgcacca atcgtacaac cgccaggcag tgaaacttgc ccctgaccaa
    57901 agcgcgctac cagcggcccc agcgcccaga tagaagcacg catggtttta accagatcgt
    57961 aaggtgcgca gaatacatta acgtcgcggg catcaatatg cacagaacca ttacgttcta
    58021 ctttcgcacc cagctggctt agcagcttca ttgatgtatc gacgtctttt agtttcggga
    58081 cgttctggat ctctaccggt tcttccgcca gtagtgcggc aaaaaggata ggcagagcag
    58141 catttttagc gccggaaatt gtgacttcgc cctggagctt cgttggcccc tgaacacgaa
    58201 atttatccat ttagtttgtt ctcagttaac aattcatatc cgctaccggc gaatcgccca
    58261 tagctcaaaa gccgttcagt ttgcgatcgc gcgcccactc cgcaggggta tacgctttga
    58321 tcgacacagc atgaatgcgg ttatccgcaa tatattccat cagcgggcca tagaccgtct
    58381 gctgtttttt aacccgactc atgccgtcaa acaactcacc cacggcaata acctgaaagt
    58441 ggctgccatc gccggaaacg tggacttcct ggagggagag agcgttcatc aacacgctct
    58501 gaatttcatt attttccatg ggatcttcaa tcatcagtta ataaaccagc gaaacatctt
    58561 agagcaaagt tgcgctggca taaataagca aaaagcctcg ctgataaatc agacaaggct
    58621 cgacttgcag gcaggtttgc cggacaggcg gttaacgcca tatccggcct gaaaaaattt
    58681 aacgaggcag aacatcagca ggcaaattat acaatttcgc cagggtatac actttgtcgt
    58741 ttaccccctg aagcgtcaca ttgttgccct gctttttcgc cagatcgata agatggagca
    58801 gcagcgccag tccacctgta tcaacgcggg atacacggct aagatcgata caggtaatcc
    58861 ccttcaccgc ttcctcacgc atttcccaaa gcggtagcag aacgtcctga tccagctctc
    58921 cggataacgc cagcgtgtca cccgtctgca tccagctcag tgactcgctc attatttttt
    58981 ctcttccaga gtgattttct gttgagaaat cgatttcagt tgcgcagtca ggccgtcaat
    59041 acctttggta cgcagcagcg ttccccactc gttttgtttg gtggtgatca tactgacgcc
    59101 ttcagcaatc atgtcgtaag cctgccagtt gcccgtctgg gagtttttac gccactggaa
    59161 gtccagacgc accggcgggc ggccattcgg gtcaataatg gtaacgcgaa taggcacaat
    59221 ggttttatca cccagcggct gttctggcgc aatctgatag gtttgaccgt gatacatcgc
    59281 cagcgcctga ccgtaagcct gcttcaggta ctcacggaaa gcggcaaagt aagcatcacg
    59341 ttgagctggg gtcgcactct tgtaatactg acccagcacc agcgcaccgg cgtatttcac
    59401 ctgtacgtat ggcagcagtt cctgatcaac aatggtacgc agataatccg ggttggcccg
    59461 aatttgtggt tgctcattct tcaggcgatc gaacgttttc tgcgccgcct cgtccatcag
    59521 cttatacgga ttggtctggt ctgccgcggt tgccgcactc agcggtgcaa tcaccagcaa
    59581 agcgaccatc attaaacgtt taaacatgcg tcggttctcc tgaaattatt tcgttgtacc
    59641 cacaggttca gtggtttcat tattacctgg cgcagcagct ggcgcatcgc cactattctt
    59701 attgtcatcg cctttactac cgtaaaggaa ctgaccaata agatcttcca gcaccatcgc
    59761 agacttggtg tcctgaattg tatcgccatc cttcaggata gcagtcccca gttccgggtc
    59821 ttcaaaaccg acgtttaatg ccagatattg ttcccccagc aggccggaag tacgaatgct
    59881 cagcgaactg gtatcaggaa tgtggttata acgttgttca atttccagcg ttacgcgcgg
    59941 cagataggtt ttcgggtcca gcgtaatatc cgccacccgg cccacaacaa caccaccaat
    60001 gctgaccgga gagcgcgctt tcaggccgcc aatgttatcg aacgtcgcat aaagcgtgta
    60061 ggtcggttca gtacgtatgg acgtcacgtt cgccgccttc aggcaaacaa acagcgccgc
    60121 cagtagtgct gctaataaaa agatacccac ccaaatttca ttttttttcg tttgcatgaa
    60181 ctcaattccc aaacatcaat gcggtcagca caaaatccag ccccagaaca gccagagacg
    60241 agtggacaac ggtgcgagtg gttgcccggc taatcccggc agacgtcggg atggcgtcgt
    60301 agccgttaaa caacgaaatc cacgtcaccg tgatggcaaa caccacgctc ttaatcagac
    60361 agttgaccag atccatacgc cagtcgacgg cattttgcat tgccgaccag aagaacccgc
    60421 tatcaatgcc tttccaactg acgccgacca gagatccgcc ccagatcccc acggcgacga
    60481 aaataaccgt caacagtggt aatgaaataa ccccagccca gaaacgggga gaaataaccc
    60541 gacgcagcgg atccaccgcc atcatctcca tactggagag ttgctctgta gcgcgcatca
    60601 ggccgatttc tgcggttagc gccgaaccag cacgcccggc aaacaacaac gcggcaacca
    60661 ccggccccag ttcacgcagt agcgataacg ccaccagcat acccagactg gtttccgcac
    60721 tataagtggt cagaaccaga taaccttgca gccccaacac cattccgatg aacacgccag
    60781 aaaccacaat aatcagcatc gacaggacgc cgacattata gagctggcgc accagcagcg
    60841 gcgcatgttt gcgaaattcc ggtttgccga ccagcgcatt gaataacatt aacccggccc
    60901 gcccgaacgt tctcagggtt ttaatccctt tatgtccgag cgacgccagc gcatttaaca
    60961 gcatgagtgg cttaactccc tggtaaaaga tcagcgtgat aatcgccggc aggatagcgg
    61021 aacggaacag gcccgtcagc tatcccgtcc agaaactgac gtacgcgtgg atcaggattc
    61081 gcctgcaacg cctgggcact gccatgagcg acaatttttt tgtccgccag gatccaggcg
    61141 tgatcggcaa tacttaacac ttccggcaca tcgtgagaaa ccaccacaca agtcacaccc
    61201 agcgcgctgt tcagctcaga aatcagcttc accagtacgc ccatggtgat gggatcttgc
    61261 ccaacaaagg gttcatcaaa catgatgaga tccggctcca gcgcaatcgc acgcgccagc
    61321 gctgcacgcc gcgccatccc accggaaagt tcagaaggca ttagtttagc cgctccacgc
    61381 agccccacgg cctccagctt catcatcacc gtactatgca acaatggcgc gggaagttgg
    61441 gtatgttcgc gcagtggata ggcgacgttg tcaaatacgt tcatatcagt gaacaacgcc
    61501 cctgactgaa ataacatgct catccgtttg cgcactgtat acaggcgcga acgagacatc
    61561 gccggaatat tctcaccatc gaaaagaatc tcaccatgat ctggtgcgat ttgcccgcca
    61621 atcagacgga gtagcgtcgt tttaccgatg cccgatggcc ccatgatcgc cgtgatcttc
    61681 cctcgcggca cggtcaggga aatattatcg aagatgcagc gattgccacg cgtaaaactg
    61741 acatcgcgca tatcgactaa attcgccaca gactgctcca taattcaccc ttcgtcttgc
    61801 gttgattttc taagcatggc gctcaattta accttgaacc caacatattt acagaatatt
    61861 acccgccgtg gttagcgaaa gctggcattt gttttacttt ttagccgcat aaagtcaaaa
    61921 ttaagaatcc gttacggctt tctgaaaatc ttcagcggac cggcgagtat acctgaagaa
    61981 aggacgttag atgcttttag ctacggcact gttaattgtt ggtttacttt tggtcgttta
    62041 cagtgccgac cgcctggttt ttgccgcgtc tattctttgc cgaacctttg gcatcccgcc
    62101 gctaatcatc ggcatgacgg tggtcagtat tggtacatcg ttaccagaaa tcatcgtctc
    62161 gcttgccgcg tctctgcacg aacaacgcga tttagccgtt ggtacagccc tcggctcaaa
    62221 cattatcaat atattgctga tcctcggtct ggctgcgctg gttcgtcctt ttaccgtcca
    62281 ttctgatgtt ctacgccgtg aattaccctt aatgttgttg gtcagcgtgg tggccggttc
    62341 cgtactctat gacggacaac ttagtcgaag cgatggtatc tttctcctct ttctggctgt
    62401 gctatggctg ctgttcattg ttaaacttgc acgtcaggct gaacgtcagg ggactgacag
    62461 cctgaccaga gagcagcttg cagagctgcc tcgtgacggc ggattgcccg tcgcgttttt
    62521 atggctcggc attgcgctta tcatcatgcc agtggccacg cggatggtgg ttgataacgc
    62581 cacggtgctg gcgaattact ttgccatcag cgagttgacg atgggtctga cggcaattgc
    62641 tatcggaacc agcctgccgg aactggcaac cgcaatagcg ggggttcgca aaggtgaaaa
    62701 cgacattgct gtcggaaata tcattggcgc aaacattttt aatattgtca tcgtattggg
    62761 tttacccgcg ctgataacgc caggagagat tgatccactg gcgtacagtc gtgactacag
    62821 cgtgatgttg ctggtgagca ttatttttgc gttgctgtgc tggcggcgct ccccgcaacc
    62881 gggccgtggt gtaggggtat tattaactgg cggatttatc gtatggctgg cgatgttgta
    62941 ctggttatcg ccaatactcg ttgaataact ggaaacgcat tatgtcgcac gtagagttac
    63001 aaccgggttt tgactttcag caagcaggta aagaagtcct ggcgattgaa cgtgaatgcc
    63061 tggcggagct tgatcaatac atcaatcaga atttcacgct tgcctgtgaa aagatgttct
    63121 ggtgtaaagg gaaagttgtc gtcatgggga tggggaaatc ggggcacatc gggcgcaaaa
    63181 tggccgcaac gtttgccagc accggtacac cttcattttt cgtccatcct ggtgaagccg
    63241 cgcatggtga tttaggcatg gtcaccccac aggatgtggt gattgctatc tctaactcag
    63301 gtgaatccag cgaaatcacg gccttaattc cagtgcttaa gcgtcttcac gtaccgttaa
    63361 tctgcatcac cggtcgcccg gagagcagca tggcgcgcgc cgcagatgtg catctgtgtg
    63421 ttaaagtagc gaaagaagcc tgtccgttag ggctggcacc gaccagcagc accaccgcca
    63481 cgctggttat gggcgatgcc ctcgctgtcg cgctgttaaa agcacgcggc tttactgctg
    63541 aagattttgc gctctcacac ccaggcggcg cactgggtcg taaacttctg ctgcgcgtaa
    63601 acgatattat gcatacgggc gatgagatcc cgcatgttaa gaaaacggcc agtctgcgtg
    63661 acgcattgct ggaagttacc cgcaaaaatc ttggtgtgac tgtcatttgc gatgacaata
    63721 tgatgattga aggcatcttt accgacggtg atttacgccg tgtcttcgat atgggcgtgg
    63781 atgttcgtca gttaagtatt gccgatgtga tgacgccggg gggaatacgt gtgcgccctg
    63841 gcattctggc cgttgaggca ctgaacttaa tgcagtcccg ccatatcacc tccgtgatgg
    63901 ttgccgatgg cgaccattta ctcggtgtgt tacatatgca tgatttactg cgtgcaggcg
    63961 tagtgtaaag attcaaggat aaacaacaat gagcaaagca ggtgcgtcgc ttgcgacctg
    64021 ttacggccct gtcagcgccg acgttatagc aaaagcagag aacattcgtc tgctgatcct
    64081 cgatgtcgat ggcgtactgt cagatggcct gatttatatg ggcaataatg gcgaagagct
    64141 gaaagcgttc aatgttcgtg acggttatgg cattcgttgt gcgctcacct ctgatattga
    64201 agtcgctatc attaccgggc gaaaggctaa actggtagaa gatcgttgtg ccacattggg
    64261 gatcactcac ttgtatcagg ggcagtcaaa caaactgatc gcctttagcg atttgctgga
    64321 aaaactggcg attgccccgg aaaatgtggc ttatgtcggc gatgatctca tcgactggcc
    64381 ggtaatggaa aaagtgggtt taagcatcgc cgtggccgat gcgcatccac tgttaatccc
    64441 gcgcgccgat tacgtgacgc gcattgctgg cggtcgtggt gcagtgcgtg aagtttgcga
    64501 cttattactc ctggcgcagg gcaaactgga tgaagccaaa gggcaatcga tatgagtaaa
    64561 gccagacgtt gggttatcat tgtgctgtca ctggcggttc tggtgatgat cggcattaat
    64621 atggccgaaa aagacgatac cgcccaggtg gtcgtcaaca acaatgatcc cacctataaa
    64681 agcgagcata cggacacgct cgtctataac ccagaagggg cactaagcta tcgattgatt
    64741 gctcaacacg ttgaatatta ttccgatcag gccgtttcgt ggtttacgca gccggtactt
    64801 accacgtttg ataaggataa aatcccgaca tggtccgtaa aagcagataa agccaagctg
    64861 accaatgacc ggatgctcta tttatatgga cacgttgaag tcaacgcact cgtgccggac
    64921 tctcaacttc gcagaatcac gacggataac gcgcagatca atctggtgac gcaggatgtt
    64981 acctctgaag acctcgtcac gttatacgga acaacattta actccagcgg tctgaaaatg
    65041 cgcggcaact tacgcagcaa gaacgccgag ctgattgaaa aggttagaac atcctatgaa
    65101 attcaaaaca aacaaactca gccttaatct tgtgcttgcc agctcacttc tggccgccag
    65161 cattccggca tttgccgtaa ccggagacac tgatcagccg atccacattg aatcggacca
    65221 gcaatctctt gatatgcaag gcaacgtggt tacctttacc ggcaatgtca tcgtcaccca
    65281 gggcaccatc aaaattaatg ccgacaaagt ggtcgttacc cgtccgggcg gcgaacaagg
    65341 taaagaagtg attgacggct acggtaaacc ggcaacgttc taccagatgc aggacaacgg
    65401 taaacccgtt gaaggtcacg cttcacagat gcactacgaa ctggcaaaag attttgtcgt
    65461 tctgacgggt aatgcttatc tgcagcaggt cgatagcaac attaagggcg ataagatcac
    65521 ttacctggtg aaagagcaga aaatgcaggc tttcagcgac aaaggcaagc gcgtaaccac
    65581 cgttctggtg ccgtcgcagc tgcaggacaa aaacaataaa ggccagaccc cggcacagaa
    65641 gaagggtaat taattcgtta tggcaacatt aactgcaaag aaccttgcaa aagcctataa
    65701 aggccgtcgc gtggtagaag acgtcagcct gaccgtcaac tccggggaaa ttgtcggtct
    65761 gctggggcca aacggtgccg gtaagaccac cactttctac atggttgttg gcattgtgcc
    65821 gcgcgatgcg ggcaacatca ttattgatga tgacgatatc agtctgctgc ctctgcatgc
    65881 acgcgcgcgc cgcggtatcg gctatctgcc gcaggaagcc tccattttcc gtcgcctcag
    65941 cgtttacgat aacctgatgg cggtactgca aattcgtgac gacttgtctg ctgaacaacg
    66001 tgaagaccgc gcgaacgagc tgatggaaga gtttcacatt gagcacctgc gtgacagcat
    66061 ggggcagtca ctctccgggg gtgaacgtcg ccgtgtagaa attgcccgtg cactggctgc
    66121 gaatccgaaa tttattctgc tcgacgaacc gtttgccggg gttgacccga tctcggttat
    66181 cgacattaaa cgcatcattg agcacctgcg cgacagcggc ctgggcgtac tgatcactga
    66241 ccacaacgtg cgtgaaacac tggcggtttg tgaacgcgct tatatcgtca gtcaggggca
    66301 tttgatcgcc cacggcacgc ctacagaaat cttacaagac gaacacgtta agcgtgtata
    66361 ccttggggaa gacttcagac tctgataggg tagaagtttg cgatgtttta gcaggagagt
    66421 acgattctga acatgaagca aggtttgcaa ctcaggctta gccaacaact ggcgatgacg
    66481 ccacagctcc aacaggcaat tcgtctgttg cagttgtcga cgctggaact tcagcaggag
    66541 ctacagcagg cgctggagag taatccgctg cttgagcaaa tcgacactca tgaagaaatc
    66601 gacacccgcg aaacgcaaga cagtgaaaca ctggacaccg ccgacgcgct cgaacaaaaa
    66661 gagatgccgg aagagctgcc gctcgatgcc agttgggaca ccatttacac cgctggtaca
    66721 ccatccggca ccagcggtga ctacattgac gacgagctgc cggtctatca gggcgaaacg
    66781 acgcagacct tgcaggatta cctgatgtgg caggtcgagc tgacaccgtt ttccgacact
    66841 gaccgcgcta ttgctacctc tatcgtcgat gccgttgatg acaccggtta tctgactgtc
    66901 ccgctggaag atattctcga aagtatgggc gatgaagaga ttgacatcga cgaagttgaa
    66961 gccgtcctta agcggatcca acggtttgat ccggtcggtg tggcggcaaa agatctgcgt
    67021 gactgtctgc tgatccaact ctcccaattc gataaaacca cgccatggct ggaagaggcc
    67081 agactgatca ttagcgatca tctcgatctg ttagccaatc acgacttccg cactttaatg
    67141 cgcgtcacgc ggctgaaaga agatgtgctg aaagaagccg tcaatctgat ccagtcgctc
    67201 gatccgcgcc ccgggcagtc gatccagacg ggcgaacctg agtatgtcat tccagatgtg
    67261 ctggtgcgta agcataacgg tcactggacg gtagaactca acagtgacag cattccgcgt
    67321 ctgcaaatca accagcacta cgcctcgatg tgcaataacg cgcgcaacga tggtgacagc
    67381 cagtttatcc gcagcaatct gcaggatgcc aaatggttga tcaagagcct ggaaagccgt
    67441 aacgatacgc tactgcgcgt gagtcgctgt atcgttgaac agcagcaagc cttctttgag
    67501 caaggcgaag aatatatgaa accgatggta ctggccgata tcgcccaggc tgtcgaaatg
    67561 catgaatcga cgatatctcg cgtgaccacg caaaaatacc tgcatagtcc acgaggcatt
    67621 tttgaactga agtatttctt ttccagtcac gtcaataccg agggcggcgg cgaagcttcc
    67681 tccacggcga ttcgtgcgct ggtgaagaaa ttaatcgcgg cggaaaaccc agcgaaaccg
    67741 ttgagcgaca gcaagttaac ctctttgctg tcggaacaag gtatcatggt ggcacgccgc
    67801 actgttgcga agtaccgaga gtctttatcc attccgccgt caaaccagcg taaacagctc
    67861 gtttgaccca accgataagg aagacactat gcagctcaac attaccggaa ataacgtcga
    67921 gatcaccgag gcactgcgcg aatttgttac agccaaattt gccaaacttg agcaatattt
    67981 tgaccgaatc aaccaggtct atgttgttct gaaagtggag aaagtcaccc acacctcaga
    68041 tgcaacactg catgtaaacg gtggtgaaat tcatgccagc gcggaaggtc aggacatgta
    68101 cgctgccatt gatggcttaa ttgataagct ggcacgccag ttgaccaaac ataaagataa
    68161 actgaaacaa cactaattgt ccgggcaatt agcatgtgca tggcggcctg ttgtgcggca
    68221 caacgggcca tttgtacggt taatgctccg agcctgttcc actgtttgag tgggcaggtt
    68281 cttaggtgaa attatgacaa ataatgatac aactctacag cttagcagtg ttcttaacag
    68341 ggaatgtacg cgaagccgcg tccactgtca gagcaaaaaa cgcgccctgg aaatcatcag
    68401 cgagctggcg gcgaaacaac ttagtctgcc gcctcaggtg gtttttgaag ctatcctgac
    68461 gcgggaaaaa atgggcagta ccggtatcgg caatggtatt gccattccgc atggcaaact
    68521 ggaagaagat actctgcgcg ccgttggcgt tttcgttcag ctcgaaacgc ctatagcttt
    68581 cgatgccatt gacaaccaac cggtagattt actatttgcg ttgctggttc cggctgacca
    68641 aaccaaaacg cacctgcata ctctgtcgct ggtggcgaaa cgtctggcgg acaaaaccat
    68701 ctgccgccgt ttacgcgcag cccagagcga tgaagagctg tatcaaatca ttacggatac
    68761 cgaaggtact ccggatgaag cgtagttatt cggtaatgtc tcttttagac gttgtgagga
    68821 gaaacagtac atggtactga tgatcgtcag cggacgttca ggttcaggta aatctgtcgc
    68881 cctgcgtgcg ctggaagata tgggttttta ctgcgtggat aaccttcccg tagtgttgtt
    68941 acccgatctg gctcgaaccc tggccgatcg cgagatttct gccgccgtca gcattgatgt
    69001 tcgtaatatg ccggagtcac cagaaatatt cgaacaggcg atgagtaacc tgcctgacgc
    69061 tttctcaccg caactcctgt tcctggatgc cgaccgtaat accttaattc gtcgttacag
    69121 tgacacgcgc cgactgcatc cgctttccag caaaaacctg tcgctggaaa gtgctatcga
    69181 caaagaaagc gatttgctgg agcctctgcg ttcgcgagcg gatctgattg tcgatacctc
    69241 agaaatgtcc gttcacgagc tggcagaaat gctgcgtacc cgtctgctgg gtaaacgtga
    69301 acgtgaactg accatggtct ttgagtcttt cggcttcaaa cacggtatcc ctatcgatgc
    69361 agattacgtc tttgacgtgc gcttcttgcc gaacccgcac tgggatccga aactgcgtcc
    69421 aatgacaggt cttgataaac ctgtcgccgc gttcctcgac cgccacacag aagtacacaa
    69481 ttttatctac cagacgcgaa gctatcttga gctatggtta cctatgctgg aaaccaacaa
    69541 ccgtagctac ctgacggtcg ccattggttg taccggcggg aagcaccgtt cggtgtatat
    69601 tgcagagcaa ctggcagact acttccgctc gcgcggtaaa aacgtccagt cacgccatcg
    69661 tacgctggaa aaacgtaaac catgaccgtc aagcaaactg ttgaaatcac aaacaagctg
    69721 ggcatgcatg cccggcctgc aatgaagctg tttgaattaa tgcaaggttt tgacgcagaa
    69781 gtgctgttac gtaatgacga aggtaccgag gccgaagcca acagcgttat tgcactgctg
    69841 atgctggatt ctgccaaagg acggcagatt gaagttgaag cgaccggtcc acaggaagag
    69901 gaagcactgg ccgccgttat cgccctcttt aattctggtt ttgatgaaga ttaatcttca
    69961 tcactttgac attcaaatcc cttcaagctc ccccgacaat aagaaaagcg cgtgctgaaa
    70021 tcgttctcaa tcaacgtcat ttgtacattt tgtgcgcttt tcactttcag aagaacctta
    70081 agaaaacctt aagaagcatt gtttaggttt cgtttaagtt aatcgaccat actggagatc
    70141 gtcagaaaat atttccagga gatggcatga ttcgcttatc tgaacaaagt cccctgggca
    70201 ccggaagaca tcggaaatgt tatgcgcatc cggaagatgc ccaacgctgt atcaagattg
    70261 tctaccatcg tggcgatggt ggcgataaag agatccgccg tgagttaaag tactacgcgc
    70321 atcttggtcg ccggttaaaa gactggagtg gaataccgcg ctatcacggt accgttgaga
    70381 cggattgcgg aaccggttat gtctacgatg tgattgcaga ttttgacggc aaaccctcca
    70441 ttacgctgac cgaatttgct gaacaatgcc gttacgaaga agacattgca caactgcgcc
    70501 agctactgaa acagctgaag cgttatttgc aggataaccg tatcgtgacg atgtcgctga
    70561 agccgcagaa tatcctctgt caccgcatca gtgagtcaga ggttatcccc gtcgtctgcg
    70621 acaacattgg tgaaagcaca ttgattccac tggcgacctg gtcgaagtgg tgttgtctgc
    70681 gtaagcaaga gcggttatgg aaacgattta tcgcccaacc ggcgctggct attgccctgc
    70741 agaaagatct gcaacctcgt gagagcaaaa cgctggccct cacttcgcgc gaagcttaat
    70801 ccagctggtg ctgttgcata aacgactcac cgcctaactg atacatctgc cgtaaaatcc
    70861 acgcctgacg gctacgcacg tagcccgatg gcgcggagac tttgaaacga agtggattag
    70921 gtaatacagc agccagtaat gcagcttccg agcgggtaag tttgctcgcg ggtttgtgga
    70981 aataacgttg tgccgcagct tcgacgccaa acacaccgtc gccaaattcg gcgatattca
    71041 ggtaaaccgt caggatacgc tttttgctcc agactgtttc tatccccagc gttaatccag
    71101 cttccagccc ttttcgtacc cagctacgcc catcccataa aaagagattt ttggccgtct
    71161 gctgagaaat cgttgaagca ccgcgaatac ggttttcatt gcgctcgttg tgcgccaggg
    71221 ctttctcaat ggaagcgaca tcaaagcccc agtgctcagg aaatttctga tcttctgcgg
    71281 caatcaccgc cagtcccatc cacggcgaga tttgatccat actgacccag tcagaatgtg
    71341 ccacgtaacg aaaattgcca tgcagccagg cgctgacctg tcgctcgacc attaccgctg
    71401 agaagggaac aggcgcaaca ctaaacaacg cgatgccccc gccccagaaa acggcgagga
    71461 caaccattaa ccgcaaaaga aaacggcgaa cgaaactaaa caccgttaag cggcttttac
    71521 tcattcagcc agaaccagca cgcgggaaac cagcttatca atgccgctcg ccgcttctgc
    71581 aatgttctgc gccagcatat atgctggggt ggtgacaatc ttattgtctt catcaaccac
    71641 gatatcatcg acaggacatg gcacatgctc cgcgcccatc tcttccagca cttctgcggt
    71701 atcgatatca gtaccgatgg tcaaacgcag cgggaaatcg aaaattttcg gcagcatcgc
    71761 cggggcaata cacataaaac caagcggttt tccggcctga tgcatcgctt gtgccagcgc
    71821 ctttaattca cggtcaacgg tgcattcgct accaagactg gcaaaattgc ttaaattctt
    71881 cgccgcgcca aaaccccccg gcacaatcaa cgcatccagt tcagcggcat cggcctgggc
    71941 cagaggacgg atttcaccgc gcgttattcg tgccgcttca atcagcacat tgcgcgtttc
    72001 cgtcatcgct tcgcccgtta aatggttgat aacatcaacc tgctgcttat ccggtgcaaa
    72061 gcagaccgcc tgcgcaccgc tgcgtgaaat agctaacaac gtcaacaccg cttcatgaat
    72121 ttcagaaccg tcatagacgc cgcatccgct cagaattacg ccaattttct tcattgtgat
    72181 catcctttcg caattgactg aaacacatta atttttttaa taaaaatggt acgcatcaca
    72241 catttaactg attcatgtaa caaatcattt aagttttgct atcttaactg cgtgcggcct
    72301 gaaaaacagt gctgtgccct tgtaactcat cataataatt tacggcgcag ccaagatttc
    72361 cctggtgttg gcgcagtatt cgcgcacccc ggtctagccg gggtcatttt ttagtggctt
    72421 ttgccaccca cgctttcagc acttctacgt cgtgacgcca ctcttctttc atctcttcaa
    72481 tccattcacc gacgttatct tcccaggcag gaaggtcagg agactgaatt tgctgaccca
    72541 gctgttgcag atggcgtaac cccactgacc ccgccgcacc tttaatttta tgtccttcct
    72601 caacaatgcc ttttttatcc tgcgccgtca gattcgactc cagcacgctg acatagcccg
    72661 gcatcatctt ctcaaacacc gctaacccgt cggtgatcag cttcggtcct acaagtgcga
    72721 gatactgttc cagcatggga atatcgagca atgcttctga tttactgttc tcttctgtcg
    72781 tcaccgtact ctcctcatca tcctgggtat cccagaattt cttgatcatc gcggttagcg
    72841 ccggaaccga aagcggctta ctcagcacat catccattcc agcattgagg tactcttgtt
    72901 tgtctttcag cacgttagcg gttaaggcca ccagcggcgg taaatcctcg cgcggataac
    72961 gcttcgtcag ttcacgagag atatccagcc cggtcatatc tggcaactga atatccagca
    73021 acaccaggtc gtattcgccc ggtttaaaca tctccagcgc cgccttgccg gtcatggcga
    73081 catcaacgct gttacctaat ttttccagca cagaacgcgc aacaatcacg ttcagttcaa
    73141 tgtcttccac cagcagcaca ttcagcgccg gtaaaggcat atcgtcttca tcaaacgcat
    73201 catcgacctc ttctgctacc gacggtgcgt ggatcgtcaa cgtaaaggtt gaacctttgc
    73261 cctgttcgct ggtaaccgta atatcgccgc ccatattttt cgccagacga cgagaaacgg
    73321 ccagaccaat accggtgccg gtggcaggtt taccgccatg actgtctttc acctggtaat
    73381 acatggcgaa aattttatcc agctcatcct gcggaatgcc gataccagag tcttccactt
    73441 caaaatgcag catatcgcct tcatcgtagc gcacgcgcac ggtaacctgg ccttgctggg
    73501 tgaatttgac ggcgttactg atgagattcc acaggatctg ccgtaaacgc gtcccgtcgg
    73561 taatgacctg atgcggtaat ggcagcgtag gctccaggtt aaagcgcaat cctttttgtt
    73621 gcgcctgcaa tgcggaaaga ttttccagat cggcaaggaa gctggtgaaa tcaaccggct
    73681 gattatcaag ctggacctta cgccgttcca tcttatccat gtcgataata tcgttgaaga
    73741 tattcccgag cgtgacggcc gaaacatgga tggttttgag atatttttcc tgctcggcgg
    73801 tgagttcggt atccagcaga atgcggctca ggccgacgat accattcagc ggtgtacgca
    73861 attcgtgact gatggtggag ataaacgtcg ttttgtcgcg gctggcccgt tcaagcgcat
    73921 cctgataccg cttacgctcg gtaatgtcgc gaccaaagcc catcaaaccg tgacgtttac
    73981 ccacgcggtc gtagtacggc actttacgga tttcaaagca ggctttgcgt ccgtcgggat
    74041 aatccagcca ctgctcatag gtcagtgaca cattatgacg gaacactttt tcatcggttt
    74101 caatgacttt cgcagcggct tccggtgagt aaacatcagc aggtttcaga tgaaccagtt
    74161 gtttttcgct ttttccggtc agcagctcca tcgcgcggtt acagccggaa aactctttat
    74221 cttcgttacg ataaaaaacc aggtcgggtg aagcgtcaag gaaggaacgt aagaatgagg
    74281 attgctgctc gagctgaatt tgtgtctctt cgcgctcttt gatttcaatt ttcagttggc
    74341 cgaaggtttc ctgcagttct gcttccgctt tttcacgaac ggcgatttcc tgatttagct
    74401 gggcaatatt atcttttaac tgaacgttga ggctcaaatc gcgctcgcgc atctcctcca
    74461 gtttttgcac cagtcgtgac agacgttgtc gtgactcctc cagttgctcg acgaccaccg
    74521 atagaaagta gaccgcccac ggcgtaatca gcaaaccaaa gaagatagaa cgaataacat
    74581 caatgctttc gacctgacca tgcagcacca tggttaccgc catttgtacc acaatggcaa
    74641 gaacgacgag ggccagcgcc agtaacattg agaagcgcac cagacctaac ttcatcatca
    74701 ggtcaacata atactgcgcc agcagacgaa tttgcttcat tagggaattc cttcacgaca
    74761 acctgcccca ataataccca attctgacag ttacgttata aattgtgtga gaaatgcgga
    74821 attcccctca atggcctacg ccgttggagg tagccacgga cgtcccagcg ccgatccctg
    74881 tacgccatgt tcattcagat aacgatccag ctcgaccatt cccgtccatc gattttcgca
    74941 ccacagcgga gcaagcagcg tcgggcgacg ggcgctggcg gaaatgcggt ggtagatcac
    75001 ttccggtggc gtatggcgaa tcatttctcc ggcagtgagc gtgtaatcct ccagttcaat
    75061 tccgttcaaa cgccccgctt cccaggcttt cgccataata ctgcctttca caatatgcag
    75121 cggatgcagc tttatgccgt ccacgccggt ttcaaccact cgctccagcg tttgcaaaca
    75181 ttccgtctgc ccttcgccag gcaggccgac aattaagtgg gaacacacct tcagcccgcg
    75241 ctgacgtgcc agctgggttg tacgctgata acaggcaaaa tcatggccgc ggttgatgcg
    75301 atgcagcgtt ttgtcgtggg cggtttgtag ccccagctcc agccacactt cgtaaccctg
    75361 gtccttatat tcgcaaagca gatccagcac cgcatccggc acgcagtccg ggcgggtacc
    75421 aacacacaaa ccgacaatat tggcctggct caccgcctgc tgatacatag aacgcagcac
    75481 ctgaacttcc gcaaaggtgc tggtatacgc ctgaaagtag gccagatagc gtttagcgcg
    75541 gttaactaaa ttcgcctggt gcgccagttg ctcggcaatg gaacgatgct gctgcgcttc
    75601 atcggcaaac gaggccacat tacagaatgt gcagccacca cgcccgatgg taccgtcacg
    75661 gttagggcag ctaaaaccgc cgtgcagcgt cagcttatgc accttttgcc cataacgacg
    75721 ggtgagatca ccaccaaaca tattgactaa tttctgtaac tgcataatct gatagaccgc
    75781 gccttgaaaa gaggccaaag cctgccattt ttagcccaat tcggcgatga cctggatcaa
    75841 tcgtcccggc ccgcttttat caactgcata atcaatcaaa attaccgaaa tttcatgcac
    75901 aatcacataa atcacttttg cttatcttgt gtcagatttt tttatctcct tatggatttt
    75961 aggcaaaaac agtagcatga aacgttatta ccaattaagg cagtataaaa tgctggtttt
    76021 ctcgtcagtt caaggcagga taagggttaa cacgccttta tgacagtcag gaattggctg
    76081 tttctctaac gacgcccctt ttagccttaa atagaaaatc cactttaatt tcagccaatt
    76141 aataaagaat tttgcgctaa agcacatttc tgtaccaata agcttgccat ttgacctgta
    76201 tcagctttcc cgataagttg gaaatccgct ggaagctttc tggatgagca gcctgctcat
    76261 catatttatg cagtaattga gatcccctct tcaccgtatt aaccgatgcg aaaaggacaa
    76321 caagggggcg aatgcgaggc gcgcgtatga cacgcaaacc ccgtcgccac gctctttctg
    76381 tgcccgtgcg cagcggttcg gaagtggggt tcccgcagag cctgggggag gttcacgata
    76441 tgttgtacga taaatccctt gagagggata actgtggttt cggcctgatc gcccacatag
    76501 aaggcgaacc tagccacaag gtagtgcgta ctgcaataca cgcactggcc cgcatgcagc
    76561 accgtggcgc gattctcgcc gatggtaaaa ccggtgacgg ttgcggcttg ctgttacaaa
    76621 aaccggatcg cttttttcgc atcgttgcgc aggagcgcgg ctggcgttta gcaaaaaact
    76681 acgccgtcgg gatgctcttc ctgaataaag atcctgaact cgccgctgcc gcacgccgca
    76741 tcgttgaaga agagctgcaa cgcgaaacgt tgtcgattgt gggctggcgt gatgtcccca
    76801 ctaacgaagg cgtgctgggt gaaatcgccc tctcctctct gccacgcatt gagcaaattt
    76861 ttgtgaacgc cccggcaggc tggcgtccgc gcgatatgga gcgccgtctg tttatcgccc
    76921 gccgccgcat tgaaaagcgt ctcgaagccg acaaagactt ctacgtctgt agcctgtcga
    76981 atctggtcaa tatttataaa ggtctgtgta tgccggcgga tctgccgcgc ttctacctgg
    77041 acctcgcgga cctgcgtctg gaatcggcta tctgcctgtt tcaccagcgc ttttccacta
    77101 acaccgtacc gcgctggccg ctggcgcagc cgttccgcta cctcgcgcat aacggtgaaa
    77161 tcaacaccat caccggtaac cgccaatggg cgcgtgcgcg tacctataaa ttccagacgc
    77221 cgctgatccc tgacctgcac gacgccgcgc cgttcgtcaa cgaaaccggc tctgactcca
    77281 gttcgatgga taacatgctg gaactcctgc tggcaggcgg gatggatatc atccgtgcca
    77341 tgcgtctgtt agtgccacca gcctggcaga acaacccgga tatggacccg gaactgcgtg
    77401 ccttctttga ctttaactcc atgcatatgg agccgtggga tggtccggca gggatcgtga
    77461 tgtccgatgg ccgtttcgcc gcctgtaacc tcgaccgtaa cggtctgcgt ccggcacgct
    77521 acgtcatcac caaagataaa ctgatcacct gcgcctctga agtcggtatc tgggattatc
    77581 agcctgacga agtggtcgaa aaaggccgcg tagggccagg cgaactgatg gtgatcgaca
    77641 ctcgcagtgg gcgcattcta cactcggcag aaaccgatga tgacctgaaa agccgccatc
    77701 cctataaaga gtggatggag aaaaacgtcc gccgtctggt accgtttgaa gatcttcccg
    77761 atgaagaagt gggtagccgc gaactggacg acgacacgct tgccagctac cagaaacaat
    77821 ttaactacag cgcggaagag ctggattcgg tgatccgcgt actgggtgaa aacggtcagg
    77881 aagcggtcgg ttcgatgggc gacgataccc cattcgccgt gctctccagc cagccgcgca
    77941 ttatttacga ctacttccgc cagcagtttg ctcaggtaac caacccgcca atcgacccgc
    78001 tgcgcgaagc gcatgttatg tcgctcgcca ccagtatcgg tcgtgaaatg aacgtcttct
    78061 gtgaagcaga aggccaggcg caccgtttaa gctttaaatc gccgattctg ctctattccg
    78121 atttcaaaca gctcacgacg atgaaagagg atcactaccg cgcagatacg ctggatatca
    78181 cctttgacgt cactaaaacc acgctcgaag cgacggtgaa agagctgtgc gacaaagccg
    78241 aaaaaatggt acgtagtggc accgtgctgc tggtgctctc cgaccggaat atcgctaaag
    78301 atcgcctgcc ggttccggct ccgatggcgg ttggcgcgat ccagacccgt cttgtcgagc
    78361 aaagcctgcg ttgcgatgcc aacatcatcg tagaaaccgc cagcgcccgt gatccgcacc
    78421 acttcgccgt gttgctgggt ttcggcgcga cggctattta tccatacctt gcctatgaaa
    78481 cgctgggccg cctggtagac acccacgcga ttgccaaaga ttatcgtacc gtgatgctca
    78541 actatcgtaa cggcatcaac aaaggcttgt acaaaatcat gtccaaaatg ggcatctcca
    78601 ccatcgcctc ttaccgctgc tcgaaactgt ttgaagcggt cggtctgcac gatgatgtcg
    78661 tgggcctgtg cttccagggg gcggttagcc gcattggggg ggctagcttt gaagacttcc
    78721 agcaggatct gctgaatctg tcgaaacgag cctggctggc gcgtaagcct atcagccagg
    78781 gcggcctgct gaaatacgtg cacggtggcg aataccacgc ctacaatccg gacgtggtgc
    78841 gcacgctgca acaggcggta cagagcggcg agtatagcga ctatcaggaa tacgcgaagc
    78901 tggttaatga gcgtccggca accacgctac gcgatctgct ggcaattacg ccgggtgaaa
    78961 acgcggtcaa cattgctgat gttgaaccgg caagcgaact gtttaaacgc ttcgataccg
    79021 ccgcgatgtc tatcggcgcg ttaagcccgg aagcccacga ggcgctggcg gaagcgatga
    79081 acagcatcgg cggtaactca aactccggcg aaggcggtga agacccggcg cgctatggca
    79141 ctaacaaagt gtcgcgcatc aagcaggtgg cgtccggtcg cttcggcgtt actccggcgt
    79201 acctggtcaa tgccgacgtc attcagataa aagtcgccca gggcgcgaag ccgggtgaag
    79261 gaggtcagtt accgggtgat aaagtcactc cttacattgc caaactgcgc tattcggtgc
    79321 cgggcgtgac gctgatctcc ccgccgccgc accacgatat ctactctatt gaggatttag
    79381 cgcagctgat tttcgacctc aagcaggtta acccgaaagc gatgatctcc gtgaagctgg
    79441 tttccgaacc gggcgtaggc accatcgcga ctggcgtggc aaaagcttat gccgatttaa
    79501 tcaccatcgc aggctatgac ggcggcaccg gcgcaagtcc gctttcatcg gtgaaatacg
    79561 caggctgtcc gtgggagctg gggcttgttg aaacccaaca ggcgctggtt gccaacggct
    79621 tgcgtcacaa gatccgtttg caggtcgatg gcggcctgaa aacgggtgtc gatatcatca
    79681 aagcagcgat tcttggcgcg gaaagttttg gcttcggcac tggcccgatg gtagcgcttg
    79741 gctgtaaata tctgcgaatt tgccacctga acaactgcgc cacgggtgta gcaactcagg
    79801 atgacaaact gcgtaagaat cactatcacg gcctgccgtt caaagtgact aattactttg
    79861 agtttatcgc ccgtgaaacc cgcgagctga tggcacagct gggggtaacg cgtctggtgg
    79921 atctgattgg tcgcaccgac ctgctgaaag agctggacgg tttcaccgcc aaacagcaga
    79981 aactggcact gtcgaagctg ctggagactg ctgagccgca tgctggtaaa gcgctctact
    80041 gcaccgaaaa caacccgccg tttgataacg gcctgctgaa cgcgcagttg ctgcaacagg
    80101 caaaaccgtt tgtcgatgaa cgccagagca aaaccttctg gttcgatatc cgcaacaccg
    80161 accgttctgt cggcgcatcg ctttcaggct atatcgccca gacgcacggc gatcaggggc
    80221 tggctgccga tcctatcaaa gcgtacttca acggcaccgc aggccagagc ttcggcgtgt
    80281 ggaacgcggg cggcgtggaa ctgtacctga ccggtgatgc caacgactat gtcggtaaag
    80341 gcatggcggg cggcttaatc gccattcgtc ctccggttgg ctcagccttc cgcagccatg
    80401 aagcgagcat tatcggcaac acctgcctgt atggcgcgac cggtggtcgt ctgtatgccg
    80461 caggccgcgc gggtgaacgt ttcggcgtgc gtaactccgg tgctatcacc gtggtagaag
    80521 gcattggcga caacggctgt gaatatatga cgggtggtat cgtctgtatt ctgggtaaaa
    80581 ccggcgttaa cttcggcgca ggcatgactg gcggcttcgc ttacgttctc gatgaaagcg
    80641 gcgatttccg caaacgcgtt aacccggaac tggtcgaggt cttaagcgtt gacgatctgg
    80701 cgatccatga agagcatctg cgcggtctta tcaccgagca tgttcagcat accggttctc
    80761 agcgcggtga agagattctg gcgaactggt caaccttcgc cactaaattt gcgctggtta
    80821 aaccgaagtc cagtgatgta aaagcactgc tgggtcaccg tagtcgtagc gcagcagagt
    80881 tgcgcgtgca ggcgcagtaa ggggtagcaa caatgagtca gaatgtttat caatttatcg
    80941 acctgcagcg cgttgatccg cctaagaaac cgctgaagat ccgcaaaatt gagtttgttg
    81001 aaatttacga gccgttttcc gaaggccagg ccaaagcgca ggctgaccgc tgcctgtcgt
    81061 gcggcaaccc atactgcgag tggaaatgcc cggtacacaa ctacatcccg aactggctga
    81121 agctcgccaa cgaggggcgt atttttgaag cggcggaact gtcgcaccag accaacaccc
    81181 tgccggaagt atgcggacgt gtctgcccgc aagaccgtct gtgcgaaggt tcctgcaccc
    81241 tgaacgatga gtttggcgct gtgaccatcg gcaacattga gcgttatatc aacgataaag
    81301 cgttcgaaat gggctggcgt ccggatctgt ccggcgtgaa acagaccggt aaaaaagtgg
    81361 cgattatcgg cgctggcccg gcgggtctgg cgtgtgcgga tgttctgacg cgcaacggcg
    81421 taaaagcggt cgtcttcgac cgtcacccgg aaattggcgg attgctgacc ttcggtattc
    81481 cggctttcaa gctggaaaaa gaggtaatga cgcgccgccg tgaaatcttc accggcatgg
    81541 gtattgaatt caaactcaat accgaagtgg gccgcgacgt gcagctggac gatctgctga
    81601 gtgattacga tgccgtgttc cttggcgtcg ggacttatca gtcaatgcgc ggcgggctgg
    81661 aaaacgaaga cgccgatggc gtgtacgcag cgctgccgtt ccttatcgcc aacaccaaac
    81721 agttaatggg ctttggtgaa acccgcgacg aaccgttcgt cagcatggaa ggcaaacgcg
    81781 tggtggtcct tggcggtggc gacactgcga tggactgcgt gcgcacgtct gtacgccagg
    81841 gggcaaagca cgttacctgt gcctatcgtc gtgatgaaga gaacatgccg ggttcccgcc
    81901 gcgaagtgaa aaacgcgcgg gaagaaggcg tagagttcaa attcaacgtc cagccgctgg
    81961 gtatagaagt gaatggcaac ggcaaagtca gcggcgtaaa aatggtgcgc acagaaatgg
    82021 gcgaaccgga tgccaaaggc cgtcgccgcg cggagattgt ggcaggttcc gaacatatcg
    82081 ttccggcaga tgcggtgatc atggcgtttg gtttccgtcc acacaacatg gaatggctgg
    82141 caaaacacag cgtcgagctg gattcgcagg ggcgcattat cgccccggaa gacaacgaca
    82201 acgctttcca gaccagcaac ccgaaaatct ttgctggcgg cgatatcgtc cgtggttccg
    82261 atctggtggt gaccgctatt gccgaaggtc gtaaggcggc agacggtatt atgaactggc
    82321 tggaagttta agcctgtccc agcgccgcct cctcagaaaa gagtcggcgc tgaaattctc
    82381 ctttctttct ggaaagcgcc tcgcataatc tgaagaaaac caatgtaata cgctcatctg
    82441 gattgttacc ggtagatgcc cggcgtatag ctgattccat tattcaacgc agcgccatat
    82501 aaagggacag ataatggaat cgctctcaga gagaacctca acaggctacc agcaaatcca
    82561 cgacggtatt attcacctgg tcgatagcgc ccggacggaa acggtacgta gcgttaacgc
    82621 gctgatgacc gcgacatact gggaaattgg ccgacgaatt gtcgaatttg aacaaggtgg
    82681 cgaggccagg gctgcgtatg gtgcgcagct aatcaagcga ctatcaaagg atttaagtct
    82741 aaggtataag cgtgggttct ctgcaaaaaa cttacgccaa atgaggcttt tttacctctt
    82801 ttttcaacat gttgaaattc gccagacagt gtctggcgaa ttaacaccat tgggaattcc
    82861 gcagacacca tctgcagaat tcccatcggc aaagatttgg cagacgctgt ctgccaaatc
    82921 tttccccctg ccctggtcca cctacgtccg tttactttcc gttaaaaacc ctgatgcccg
    82981 cagcttttat gaaaaagaga cgctccgctg tggttggtct gtccgccagc tagagcggca
    83041 aattacgacc caattttatg agcgaacact actgtcacat gacaaatcag ccatgctgca
    83101 acaacacgct cctgccgaga tgcatattct tccgcaacag gcgatacgcg atccctttgt
    83161 gctcgaattt ctggagttga aagatgaata ctcagaatct gattttgagg aggcgctgat
    83221 caaccacctg atggatttca tgctggaact tggggatgat tttgcctttg ttggtcggca
    83281 gcgaaggtta cgcattgatg acaactggtt tcgggtcgat ctgctgtttt tccaccgccg
    83341 tttacgctgc ctgctaatcg tcgatctaaa agtgggcaaa ttcagctata gcgatgccgg
    83401 acagatgaat atgtatctca actacgccaa agagcactgg acactaccgg atgaaaatcc
    83461 gcccatcggt ctggttctct gtgcagagaa aggagccggc gaagcgcatt atgcactggc
    83521 tggtttgcct aacaccgttc tggcgagtga atataagatg caactacctg atgaaaaacg
    83581 actcgcggat gaactcgttc gaacacaggc ggtgttagag gaaggctata gacgccgtta
    83641 atttcagcgg tttcgaattc tccagacact gtctggagaa tctttaaaac gaactaagtt
    83701 ttaagccatt aaatcagcct taaccttcac cacaaccttt ttaatctcac caggttcgcc
    83761 gacaacgcat cctggtttat gcggttctcc cggcataaac acggcgaaca ttcccggttt
    83821 taagatgatg gcttgctcgt tctcaatggc gctgcaaagc tggtaatcat cctcatggtg
    83881 gaactcttca cactgacgcg cagtgcctgc cgtgccaaac agaatccgtt cctcaccgtt
    83941 taataacagc tggatatcaa tgtattgctc gtgcagctcc gcttttttct caacgggcga
    84001 ttgagtgtta aacgtcatga cattcataaa gatattgtcg cactgtaatt cgtaacgacc
    84061 cggtgctttt tcttgcggcc tggcggctaa ggctagtgtt aacgcgtcct gtaacgcagg
    84121 atgcaatccg gcagacggta acgactgtac ttcacccatt atcataattt ttctccctgg
    84181 gccaacaacg cagccccaag taaacctgca tcgtggcggt agtgcgccgc cagtaaatca
    84241 acatgaaatg ccgctggctc ctgcgccaga tacgtttcca ccaacgccag atacccttct
    84301 gccagaccaa cgctgccacc gaccactacg cactggcaat cagttgtggc tttaatatca
    84361 gcgatcagcc ttgccagcac atgtgcggag cagtgaatca gttgctgcgc ctgctcatcg
    84421 ccctgcccgg cgcgtgtgaa aatagttctc gcatccgcgc ccgccagttc accctgcgct
    84481 gccgctgcaa taccgcgacc agaagcaatc gcttccacac aaccagtgcg tccacagccg
    84541 cagactggac catgaggatc agccagcgta tgcccgatgt gccccgccag accgccaggg
    84601 ccggtgagca gtttgccgcc gctcactaca ccgccgccaa caccagtgga aacggtgata
    84661 aacaccatct cggttatatc gccttccagc gcctgatact ccgcccacgc tgcggcctgc
    84721 gcgtcgttaa tggcaatggt cggcaaatcg gtaagttgtt ccaaagtttt gactaacgga
    84781 aagtgtagca atccaccgag attatgcgga ttcagcgcca gcaggctgcc gtcgcgaata
    84841 attccggttg aagcgatagc aacccgctgc gcatgaactt gcaacggaga gactaatgcg
    84901 gataaggcat catgcaaggc ttctggtgtc tggctggctg gcgtaggaag ttcacgacga
    84961 tcgcggatct gcccatcagc gccaatcagc gcggcggcaa gtttagtacc gccgatatca
    85021 atcgccagtg tggtcatagc accgcctttt tcatcgctgt gttgtaccac tgacaaatgt
    85081 gttcaagacg cgtaatggca gaaccgaccg tcaccgccca cgcgccgtgg cgcatcgcat
    85141 ctgccgcctg agcaggcgtg ttgtaacgcc cttcggcaat cacccgacat ccggcgtcgc
    85201 tcaacgtttt caccagcgcc agatccggct cttctggcgt ttcaggcgtg gtatagccag
    85261 aaagcgtagt gccaataatt tcggctccca gcttttggca tgccaggccg tcttccggcg
    85321 ttgagcagtc ggtcatcgcc agtaaaccgt gatggtgaat acgtgccagc agcgtttcaa
    85381 caggcaccgg acgcggtcgg tcggtgccgt caatggcgat aatgtccgcg cccgcctgcg
    85441 ccagcgcatc aacatcttca atataggccg tgatgcgtac cggagaatcc tccagatcgc
    85501 gtttcacaat cccgataatt ggcacgctca ccaccgcacg cgtggcttgc agatttgcca
    85561 caccttcaat gcgaatggca accgcgcccg cctgttctgc cgctaatgcc atggcggcga
    85621 cgatttcggg tttatcgagc gggctgtccg gaaccggctg gcaggagaca atcaggccac
    85681 cgttagcagc gattttttga tccagttgtg caagtaacga catacatctt cccttagcga
    85741 aaggcccggt acagagaccg ggcaacagga ttaacttttg gttttgacta aatcgttttt
    85801 ggcgcggcca aatggcacgg caccgctaaa tggtttgccg tcgatagcgt catgagtacg
    85861 caatgcttcc gggcgcagcc aacgctgaac gcgggaaggc atatccagac caatcagcag
    85921 gatcaccaca aatgtcagac tgaacgagag cgatgccagc gcagtaccca gatccagacg
    85981 ttgagcgatc aacgcgccga tgattggggc cagtgcaccg cccaatgcgc cgacgttgta
    86041 ggtaaagccc aggcccgctg cacgctggtc ggtatcgaaa taaccgccaa tcagtttcgg
    86101 taagatcccg gcgatccctt gtccaagcat ttgctggaag aacagtaaca gaccgagcac
    86161 ccagacgttt gcgccgccaa tcgcaaatac cggaataatc agcagctgcg aggccagcag
    86221 actacaaacg tacgctttgc gggttcccag ccagtcaccg aggaagccac cgacgcagca
    86281 ccccaccgcc gcgccaaagc cactaaagaa cagcacattg gcgacagtat gcgggtcata
    86341 agccagatcg gttttcagat acgttggcag cagcgcctga atcggccatg agtagaggaa
    86401 agcaaacaac acgaccacca tcagcattac gcccgttggc cagcgtttgc ccgtactctg
    86461 caccataaag ctgataaaga ttgcggcgca taacagcccg agtacagcga cgattgcggc
    86521 attttgcagg ttaccggcga agcagaacca cagcgcagta gccgccgcca gcgtcattac
    86581 gatattggca atgcgatgtt caccacggta aagaatgtct accatcgtgc gtactggtgc
    86641 tttacccccg tgtttctctt tccagtcttc cgcttccggg atgtttttac gcagccagag
    86701 agcaaagatg attggcaaaa tgccgataaa gaacagcgct ctccagcccc agaccggaac
    86761 caccagacta tagacctgag cggcaacgac ggcccccaca gagaagcctg aaatcaaaaa
    86821 accactggct ttgttacgca gatgttttgg ccagctttca atgacatagg tggcgctgga
    86881 accgtattca cccgccatcc ccatgccgat gaccagacgc gcgataaaca tggtgatgta
    86941 gcctggcgca aagccgcagg ccagcgtccc ggccgagaag agaacgatgc tggtgaccat
    87001 tgccagacga cgcccgtagc ggtcacccat agcgccgagc atcaggccgc cgaaccagcg
    87061 agagataaag gctgcggaga tcagacttgc cgcctgcacc gtcgtcagcc cgaattcacc
    87121 ctgtacttcg gtgagtacca gggcgattaa aacgaaatca aaaccgtcaa gcagatatcc
    87181 caaccaggca gcggaaaatg cgcgccattg tgcacggttg agatggcgat accacgggat
    87241 attctgggtt gtagtactca ttgtgagtct cccgcggtgg gcgatgccca cacgctttgg
    87301 tatgaaaatt gtagggtaca gatgcgttta tttcccctca cccggtaggg gcgagcgagg
    87361 ggaaaacaac tcacccgcgc tcttgcatca actgctgggc cagcgccttc agttctggca
    87421 gatatttttc atctaccggt ccaaacggtt tgcggcacag cggcacagaa acgacatcca
    87481 tataatgcag gacagttttc aggccgcgga atacgcccgt tttgatcagt aaatcaatga
    87541 ctttgttgca ctcagtttgc agtttctgcg cggtctggat atcgccttct ttcagcgcct
    87601 tcacgatccc ctgatatcgc cagcccatga tgttgtaagt actgccgata ccaccatcag
    87661 cgcctgccag caagccggaa gcgaagattt cgtcgtaacc gttatagagc accagatcag
    87721 gatgttcacg acggatctgt tccatctgat agagatcgcc agaagtctgt ttcagcgcac
    87781 cgacgccagg caacgtaacc agggtgttga tctgatccag ggtcagtttt accccactca
    87841 gggctggaat gttgtacacc accatcggca aaccatccgc cgaatcaata attgcccgat
    87901 agtgatcgca gtgttcttca aagctgaaag gatagtagaa cggcgtgacg gcggagacgg
    87961 catcgaagcc ataacgttta gccgatgccg caagttgttg gctttcggcg gtgctgacgc
    88021 aaccgacgtg ggcgatgagt ttaatcttac ctttcgcctc ttcagcgacg atttccagta
    88081 cctgttcacg ctcggaaagg ctttgtacaa aggcttcgcc ggtagaacca cccacgtata
    88141 aaccgtcgat gccctgctga atattgaact gaaccaggcg acgcagactc gctttatcca
    88201 gtgcttgttg ttgatcaaaa ggagtcagga gtgcggccat tacgccacgt aaattcgttg
    88261 ccataaatac ctctgaagtg atgcttatct gataaacgat atacctttat acctgttata
    88321 ccagatcaat taagcaacac cccatacaga aagcttataa tgcgatctgc ttcactaaag
    88381 tggcattatt tctttttgtt ggtggtctga ccgaaggcgt gccaggtagc agagacgctg
    88441 ttgagatgcg attgcaacgc acgatcggct tcatcgggat cgtggcggcg gatcgcgtca
    88501 acgatcgcaa tatgctgttg atagctaacg ttgttatgct cgtgcagagc ctgatcagcc
    88561 acggttggac gtgcggcgat cagccagtcg agcaaggcaa cgtggatcgc catgaagatt
    88621 gggttaccgg ggatctccgc cagcacgcgg tggaaatcaa cgtctgaacg aatgaatgcc
    88681 gcgttgttat ccagcgactg actgttgatt tccagtgctt ttgccagcaa atcgatttgc
    88741 tcatcggtgg cgtgttcagc cgcatagcgc accaggctgg attcaaagaa cagacgtaat
    88801 tgttcgaaat gggcaatccc gccaggatga gaaaggaaat ctttcgccat gccggaaagc
    88861 tcgccgatga tggtgtccgc agaaggacgt gagacgcgag cgcgttcgcc gttgtttatc
    88921 tgcaccagac ctttgcgttt taacgctgcc agcgcttcgc gcaccgaagg acgcccgacg
    88981 ttaaagaacg ccatcagttc gcgttcagac ggtaattgtt caccttcgcc aaattcacga
    89041 cggcggatca tctgttccag ctcttcttcc accatttcgg agagtttttt acgcgccagc
    89101 gggcggctgc gtaagttgcg gccaattgca ggtgaagaat cttcggtttg cgaatcaaat
    89161 gcgttcataa ggcccattct gtaaggtcag tgtgattaac atcatcagtg acatcctatc
    89221 acaggattga aagtaggggg aaatggcggg gtttt
//
LOCUS       DAZGQL010000006        83237 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.006, whole genome shotgun sequence.
ACCESSION   DAZGQL010000006 DAZGQL010000000
VERSION     DAZGQL010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 83237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 83237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..83237
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.006"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(381..1415)
                     /locus_tag="ACHKIR_000488"
     CDS             complement(381..1415)
                     /locus_tag="ACHKIR_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001610013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO9988280.1"
                     /translation="MSKKKGKTPRPAAKTMTASAPKMEAFTFGEPVPVLDRRDILDYV
                     ECISNGRWYEPPVSFTGLAKSLRAAVHHSSPIYVKRNILASTFIPHPWLSQQDFSRFV
                     LDFLVFGNAFLEKRYSTTGKVIRLETSPAKYTRRGVEEDVYWWVPSFNEPTPFAPGSV
                     FHLLEPDINQELYGLPEYLSALNSAWLNESATLFRRKYYENGAHAGYIMYVTDAVQDR
                     NDIEMLRENMVKSKGRNNFKNLFLYAPQGKADGIKIIPLSEVATKDDFFNIKKASAAD
                     LLDAHRIPFQLMGGKPENVGSLGDIEKVAKVFVRNELIPLQDRIREINGWLGQEVIRF
                     KNYSLDTDND"
     gene            complement(1415..3187)
                     /locus_tag="ACHKIR_000489"
     CDS             complement(1415..3187)
                     /locus_tag="ACHKIR_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase ATPase subunit family protein"
                     /protein_id="HFO9988281.1"
                     /translation="MTITTDTTLLHDPRRQAALLYWQGFSVPQIAAMLQMKRPTVQSW
                     KQRDGWDSVAPISRVEMSLEARLTQLIIKPQKTGGDFKEIDLLGRQIERLARVNRYSQ
                     TGNEADLNPNIANRNKGGRRKPKKNFFSDEAIEKLEQIFFEQSFDYQLHWYRAGLEHR
                     IRDILKSRQIGATFYFSREALLRALKTGHNQIFLSASKTQAYVFREYIIAFARLVDVD
                     LTGDPIVLGNNGAKLIFLGTNSNTAQSHNGDLYVDEIFWIPNFQVLRKVASGMASQSH
                     LRSTYFSTPSTLAHDAYPFWSGELFNRGRASAAERVEIDVSHNALAGGLLCADGQWRQ
                     IVTIEDALKGGCTLFDIEQLKRENSADDFKNLFMCEFVDDKASVFPFEELQRCMVDTL
                     EEWEDYAPFAANPFGSRPVWIGYDPSHRGDSAGCVVLAPPVVAGGKFRILERHQWKGM
                     DFATQAESIRKLTEKYNVEYIGIDATGLGVGVFQLVRSFYPAARDIRYTPEMKTAMVL
                     KAKDVIRRGCLEYDVSATDITSSFMAIRKTMTSSGRSATYEASRSEEASHADLAWATM
                     HALLNEPLTAGISTPLTSTILEFY"
     gene            3361..4215
                     /locus_tag="ACHKIR_000490"
     CDS             3361..4215
                     /locus_tag="ACHKIR_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001074418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HFO9988282.1"
                     /translation="MAKKVSKFFRIGVEGDTCDGRVISAQDIQEMAETFDPRVYGCRI
                     NLEHLRGILPDGIFKRYGDVVELKAEKIDDDSALKGKWALFAKITPTDDLIAMNKAAQ
                     KVYTSMEIQPNFANTGKCYLVGLAVTDDPASLGTEYLEFCRTAKHNPLNRFKLSPENL
                     ISVATPVELEFEDLPETVFTALTEKVKSIFGRKQASDDARLNDVHDAVTAVAEHVQEK
                     LSATEQRLAEMETAFSALKQEVTDRADETSQAFTRLKNSLDHTESLTQQRRSKATGGG
                     GDALMTNC"
     gene            4274..5347
                     /locus_tag="ACHKIR_000491"
     CDS             4274..5347
                     /locus_tag="ACHKIR_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
                     /protein_id="HFO9988283.1"
                     /translation="MRQETRFKFNAYLSRVAELNGIDAGDVSKKFTVEPSVTQTLMNT
                     MQESSDFLTRINIVPVSEMKGEKIGIGVTGSIASTTDTAGGTERQPKDFSKLASNKYE
                     CDQINFDFYIRYKTLDLWARYQDFQLRIRNAIIKRQSLDFIMAGFNGVKRAETSDRSS
                     NPMLQDVAVGWLQKYRNEAPARVMSKVTDEEGHTTSEVIRVGKGGDYASLDALVMDAT
                     NNLIEPWYQEDPDLVVIVGRQLLADKYFPIVNKEQDNSEMLAADVIISQKRIGNLPAV
                     RVPYFPADAMLITKLENLSIYYMDDSHRRVIVENPKLDRVENYESMNIDYVVEDYAAG
                     CLVEKIKVGDFSTPAKATAEPGA"
     gene            5351..6094
                     /locus_tag="ACHKIR_000492"
     CDS             5351..6094
                     /locus_tag="ACHKIR_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase endonuclease subunit"
                     /protein_id="HFO9988284.1"
                     /translation="MTSPAQRHMMRVSAAMTAQREAAPLRHATVYEQMLVKLAADQRT
                     LKAIYSKELKAAKKRELLPFWLPWVNGVLEQGKGAQDDILMTVMLWRLDTGDIAGALE
                     IARYALKYGLTMPGKHRRTPPYMFTEEVALAAMRAHAAGESVDIRLLTETLELTATAD
                     MPDEVRAKLHKITGLFLRDGGDAAGALAHLQRATQLDCQAGVKKEIERLERELKPKPE
                     PQPKAATRVPRKTRSVTPAKRGRPKKKAS"
     gene            6194..6703
                     /locus_tag="ACHKIR_000493"
     CDS             6194..6703
                     /locus_tag="ACHKIR_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HFO9988285.1"
                     /translation="MMTLIIPRKEAPVSGEGTVVIPQPAGDEPVIKNTFFFPDIDPKR
                     VRERMRLEQTVAPARLREAIKSGMAETNAELYEYREQKIAAGFTRLADVPADDIDGES
                     IKVFYYERAVCAMATASLYERYRGVDASAKGDKKADSIDSTIDELWRDMRWAVARIQD
                     KPRCIVSQI"
     gene            6703..6906
                     /locus_tag="ACHKIR_000494"
     CDS             6703..6906
                     /locus_tag="ACHKIR_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HFO9988286.1"
                     /translation="MKTFALQGDTLDAICVRYYGRTEGVVETVLAANPGLAELGAVLP
                     HGTAVELPDVQTAPVAETVNLWE"
     gene            6910..7191
                     /locus_tag="ACHKIR_000495"
     CDS             6910..7191
                     /locus_tag="ACHKIR_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000123123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFO9988287.1"
                     /translation="MTAEEKSVLSLFMIGVLIVVGKVLAGGEPITPRLFIGRMLLGGF
                     VSMVAGVVLVQFPDLSLPAVCGIGSMLGIAGYQVIEIAIQRRFKGRGKP"
     gene            7191..7688
                     /locus_tag="ACHKIR_000496"
     CDS             7191..7688
                     /locus_tag="ACHKIR_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 104 protein"
                     /protein_id="HFO9988288.1"
                     /translation="MPVINTHQNIAAFLDMLAVSEGTANHPLTKNRGYDVIVTGLDGK
                     PEIFTDYSDHPFAHGRPAKVFNRRGEKSTASGRYQQLYLFWPHYRKQLALPDFSPLSQ
                     DRLAIQLIRERGALDDIRAGRIERAISRCRNIWASLPGAGYGQREHSLEKLVTVWRTA
                     GGVPA"
     gene            7703..8128
                     /locus_tag="ACHKIR_000497"
     CDS             7703..8128
                     /locus_tag="ACHKIR_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNZ54_00345 family protein"
                     /protein_id="HFO9988289.1"
                     /translation="MKKLSLSLMLNVSLALMLALSLIYPQSVAVSFVATWAILATVIC
                     VVAGGVGVYATEYVLELYGRELPPESLAVKIVTSLFLQPVPWRRRAAALVVMVATFIS
                     LVAAGWIFTALIYLVASLFFRLIRKACRQRLEGRELCQS"
     gene            8116..8541
                     /gene="lysB"
                     /locus_tag="ACHKIR_000498"
     CDS             8116..8541
                     /gene="lysB"
                     /locus_tag="ACHKIR_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585210.1"
                     /note="The gene for this Rz-like phage lysis system
                     protein may overlap extensively with the gene for the
                     other spanin subunit, the Rz1-like protein in the outer
                     membrane; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz-like lysis system protein LysB"
                     /protein_id="HFO9988290.1"
                     /translation="MSKLMIVLVVLLSLAVAGLFLVKHKNASLRASLDRANNVASEQQ
                     TTITMLKNQLHVAITRADKNELAQVALRQELENAAKREAQREKTITRLLNENEDFRRW
                     YGADLPDAVRRLHQRPACADASDCPQRLPESESLPDAGQ"
     gene            8441..8686
                     /gene="lysC"
                     /locus_tag="ACHKIR_000499"
     CDS             8441..8686
                     /gene="lysC"
                     /locus_tag="ACHKIR_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015979584.1"
                     /note="LysC is an Rz1-like component of a phage lytic
                     system, substantially overlapping although not fully
                     embedded in the gene for the Rz-like LysB component;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1-like lysis system protein LysC"
                     /protein_id="HFO9988291.1"
                     /translation="MLCAGCTSARPAQTPVIVHNACPKVSLCPMPGSDPQKNGDLSAD
                     IRLLENALARCASQVKMIKHCQDENDAQTRQPAQGAD"
     gene            8649..9116
                     /locus_tag="ACHKIR_000500"
     CDS             8649..9116
                     /locus_tag="ACHKIR_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001406878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9988292.1"
                     /translation="MLKPDSLRRALTDAVTVLKTSPEMLRIFVDNGSIASTLATSMSF
                     EKRYTLNVIVTDFTGDFDLLIVPVLAWLRENQPDIMTTDEGQKKGFTFYADINNDSSF
                     DISISLMLTERTLVSEVDGALHVKNIPEPPPPEPVTRPMELYINGELVSKWDE"
     gene            9109..9561
                     /locus_tag="ACHKIR_000501"
     CDS             9109..9561
                     /locus_tag="ACHKIR_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HFO9988293.1"
                     /translation="MNEFKRFEDRLTGLIESLSPSGRRRLSTELAKRLRQSQQRRVMA
                     QKAPDGTPYAPRQQQSARKKTGRVKRKMFAKLITSRFLHIRASPEQASMEFYGGKSPK
                     IASVHQFGLSEENRKDGKKIDYPARPLLGFTGEDVQMIEETILAHLER"
     gene            complement(9633..10418)
                     /locus_tag="ACHKIR_000502"
     CDS             complement(9633..10418)
                     /locus_tag="ACHKIR_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001695630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9988294.1"
                     /translation="MFSDNVINAWWFISLYLFLLIALTFVTFGKSNLMRFIAHHFNLE
                     YSDRKLKMLDKKWRDIQLFKTINGINVSGIEDVRMIQQGLIDGKLKTSYFFLTRFWGD
                     ITKPPHIIKTIIVILASIIYILFACYIHNKQSAIVRDAIGIPYKNMMYYVYSDKVLLS
                     FKNKTVELNKTYSLADCKTLQNVFIKDTLPEIACNKLLQLNEEDSEWLSQEIKDNNSH
                     KKALLILSLVYFISGLLIFLSYTKFLYANKKVLEYKASNKNHS"
     gene            10502..11137
                     /locus_tag="ACHKIR_000503"
     CDS             10502..11137
                     /locus_tag="ACHKIR_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFO9988295.1"
                     /translation="MNTLANIQELARALRNMIRTGLVVETDLNAGRCRVQTGGMCTDW
                     LQWLTHRAGRSRTWWAPSVGEQVLILAVGGELDTAFVLPGIYSGDNPAPSASADALHI
                     RFPDGAVIEYEPETSALTVSGIKTASVTASDSVTATVPVVMVKASTRITLDTPEVVCT
                     NRLITSTLEVQKGGTMRGNIEHTGGELSSNGKVLHTHKHPGDSGGTTGSPL"
     gene            11134..11481
                     /locus_tag="ACHKIR_000504"
     CDS             11134..11481
                     /locus_tag="ACHKIR_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HFO9988296.1"
                     /translation="MTARYLGMNRSNGLTVTDLEHISQSIGDILRTPVGSRVMRRDYG
                     SLLASMIDQPQTPALELQIKVACYMAVLKWEPRVTLSSVTTARSFDGRMTVTLTGQHN
                     DTGQPLSLTIPVS"
     gene            11486..12394
                     /locus_tag="ACHKIR_000505"
     CDS             11486..12394
                     /locus_tag="ACHKIR_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001121472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate assembly protein"
                     /protein_id="HFO9988297.1"
                     /translation="MPIIDLNQLPAPDVVEELDFETILAERKATLISLYPEDQQEAVA
                     RTLTLESEPLVKLLEENAYRELLWRQRVNEAARAVMLACAAGNDLDVIGANYNTTRLT
                     ITPADDSTIPPTPAVMESDTDYRLRIQQAFEGLSVAGSVGAYQYHGRSADGRVADISV
                     TSPSPACVTISVLSRENNGVASEDLLAVVRNALNGEDVRPVADRVTVQSAAIVEYQIN
                     ATLYLYPGPESEPIRAAAVKKLEAYITAQHRLGRDIRLSAIYAALHVEGVQRVELAAP
                     LADIVLNSTQASFCTEYRVVTGGSDE"
     gene            12387..12917
                     /locus_tag="ACHKIR_000506"
     CDS             12387..12917
                     /locus_tag="ACHKIR_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HFO9988298.1"
                     /translation="MSDSRLLPTGSSPLEVAAAKACAEIEKTPVSIRELWNPDSCPAN
                     LLPWLAWAFSVDRWDEKWPEATKRAVIRDAYFIHCHKGTIGAIRRVVEPLGYLINVTE
                     WWETSDPPGTFRLDIGVLESGITEAMYQEMERLIADAKPASRHLIGLNITRDIPGYLF
                     AGGVAYDGDVITVYPG"
     gene            12928..14973
                     /locus_tag="ACHKIR_000507"
     CDS             12928..14973
                     /locus_tag="ACHKIR_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9988299.1"
                     /translation="MSTKFKTVITTAGAAKLAAATAPGGRKVNITTMAVGDGGGKLPV
                     PDAGQTGLIHEVWRHTLNKISQDKRNSNYIIAELVIPPEVGGFWMRELGLYDDAGTLI
                     AVANMAESYKPALAEGSGRSQTCRMVIIVSSVASVELTIDTTTVMATQDYVDDKIAEH
                     EQSRRHPDASLTAKGFTQLSSATNSTSETLAATPKAVKAAYDLANGKYTAQDATTARK
                     GLVQLSSATNSMSETLAATPKAVKTVMDETNKKAPLNSPALTGTPTTPTARQGTNNTQ
                     IANTAFVMAAIAALVDSSPDALNTLNELAAALGNDPNFATTMTNALAGKQPKDATLTA
                     LAGLATAADRFPYFTGNDVASLATLTKVGRDILAKSTVAAVIEYLGLQETVNRAGNAV
                     QKNGDTLSGGLTFENDSILAWIRNTDWAKIGFKNDADSDTDSYMWFETGDNGNEYFKW
                     RSRQSTTTKDLMNLKWDALYVLVKALFSSEVKISTVNALRIFNSSFGAIFRRSEENLY
                     IIPTRENEGENGDIGSLRPFGINLRTGVVSVGNGARIDGGLALGTNNALGGNSIVLGD
                     NDTGFKQNGDGNLDVYANNVHVMRFVSGSIQSNKTINITGRVNPSDYGNFDSRYVRDI
                     RLGTRVVQTMQKGVMYEKAGHVITGLGIVGEVDGDDPAVFRPIQKYINGTWYNVAQV"
     gene            14977..15504
                     /locus_tag="ACHKIR_000508"
     CDS             14977..15504
                     /locus_tag="ACHKIR_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001762409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO9988300.1"
                     /translation="MQHLKNITAGNPKTVAQYQLTKNFDVIWLWSEEGKNWYEEVSNF
                     QEDTIKIVYDENNIIVGITRDASTLNPEGFSVVEVPDITSNRRADDSGKWMFKDGAVI
                     KRIYTADEQEQQAESQKAALLSEAESVILPLERAVRLNMATDEERSRLEAWERYSVLV
                     SRVDPANPEWPEMPQ"
     gene            15740..16324
                     /locus_tag="ACHKIR_000509"
     CDS             15740..16324
                     /locus_tag="ACHKIR_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019105494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFO9988301.1"
                     /translation="MDKKFKNQIVDSDIARLLLKHRKARNLTVTELAQRSGVSQAMIS
                     KIERGISSPSATILSRLANAMNVTLSKLFAELEQHQNSLVRFAHQQQHWTDEQSGITR
                     WSLSPAGACPELIKVEIPAMGELTIPASANEHLSGQTLWMMSGSLDFRVKNQTHHLRA
                     GDCLALTFASEYQMSNPDSEQSCSYIVAFSQKNQ"
     gene            complement(16345..16788)
                     /locus_tag="ACHKIR_000510"
     CDS             complement(16345..16788)
                     /locus_tag="ACHKIR_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019105495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFO9988302.1"
                     /translation="MLDIRMSRPSEASEIIQIWKSSVDATHDFLTGHDRQEIEKEVIG
                     FFSETPVWVATNQDDQPLGFMFLHEGHLEALFVAGSARGLGVGKRLISHALALHPELS
                     VDVNEQNQQAVGFYQHMGFQVSGRSDLDNQGRPYPLLHLRMANNM"
     gene            17201..18391
                     /locus_tag="ACHKIR_000511"
     CDS             17201..18391
                     /locus_tag="ACHKIR_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001483446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath protein"
                     /protein_id="HFO9988303.1"
                     /translation="MSDYHHGVQVLEINDGTRVISTVSTAIVGMVCTASDADAETFPL
                     NKPVLITNVQSAIAKAGKKGTLAASLQAIADQSKPVTVVVRVEDGTGDDEETKLAQTV
                     SNIIGTTDENGQYTGLKALMGAESVTGVKPRILGVPGLDTKEVAVALASVCQELNAFG
                     YISAWGCKTISEVKAYRQNFSQRELMVIWPDFLAWDTVTSTTATAYATARALGLRAKI
                     DQEQGWHKTLSNVGVNGVTGISASVFWDLQKPGTDADLLNEAGVTTLVRRDGFRFWGN
                     RTCSDDPLFLFESYTRTAQVLADTMAEAHMWAIDKPITATLIRDIIDGINAKFRELKN
                     NGYIVDGTCWFSEESNDAETLKAGKLYIDYDYTPVPPLENLTLRQRITSRYLASLVTS
                     VNSN"
     gene            18404..18922
                     /locus_tag="ACHKIR_000512"
     CDS             18404..18922
                     /locus_tag="ACHKIR_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HFO9988304.1"
                     /translation="MAMPRKLKLMNVFLNGYSYQGVAKSVTLPKLTRKLENYRGAGMN
                     GSAPVDLGLDDDALSMEWSLGGFPDSVIWELYAATSADAVPIRFAGSYQRDDTGETVA
                     VEVVMRGRQKEVDTGEGKQGEDTESKLPVICTYFKLTMDGKELVEIDTINMIEKVNGV
                     DRLEQHRRNIGL"
     gene            18979..19254
                     /locus_tag="ACHKIR_000513"
     CDS             18979..19254
                     /locus_tag="ACHKIR_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001719230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098003 - viral tail assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HFO9988305.1"
                     /translation="MNKENVITLDNPVKRGEQVIEQVTLMKPNAGTLRGVSLAAVANS
                     EVDALIKVLPRMTAPMLTEQEVAALELPDLVALAGKVVGFLSPNSVQ"
     gene            19251..19406
                     /locus_tag="ACHKIR_000514"
     CDS             19251..19406
                     /locus_tag="ACHKIR_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HFO9988306.1"
                     /translation="MTFPKNLSVDDLMADVAVIFHWPPSELYPMSLTELITWREKALR
                     RSGSTNE"
     gene            19399..21846
                     /locus_tag="ACHKIR_000515"
     CDS             19399..21846
                     /locus_tag="ACHKIR_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO9988307.1"
                     /translation="MSNNVKLQVLLRAVDQASRPFKSIRTASKSLSGDIRETQKSLRE
                     LNGHASRIEGFRKTSAQLAVTGHALEKARQEAEALATQFKNTERPTRAQAKVLESAKR
                     AAEDLQAKYNRLTDSVKRQQRELAAVGINTRNLAHDEQGLKNRISETTAQLNRQRDAL
                     ARVSAQQAKLNAVKQRYQAGKELAGNMASVGAAGVGIAAAETMAGVKLLMSGYEFAQK
                     NSELQAVLGVAKDSAEMTALRKQARQLGDNTAASADDAAGAQIIIAKAGGDVDAIQAA
                     TPVTLNMALANRRTMEENAALLMGMKSAFQLSNDKVAHIGDVLSMTMNKTAADFDGMS
                     DALTYAAPVAKNAGVSIEETAAMVGALHDAKITGSMAGTGSRAVLSRLQAPTGKAWDA
                     LKELGVKTSDSKGNTRPVFTILKEMQASFEKNRLGTAQQAEYMKTIFGEEASSAAAVL
                     MTVASTGKLDKLTAAFKASDGKTSELVNIMQDNLGGDFKEFQSAYEAVGTDLFDQQEG
                     ALRKLTQTATKYVLKLDGWIQKNKSLASTIGIIAGGALVLTGIIGAIGLVAWPVITGI
                     NAIIAAAGAMGAIFTTVGSAVMTAIGAISWPVVAVVAAIVAGALLIRKYWEPVSAFFG
                     GVVEGLKAAFAPVGELFTPLKPVFDWLGEKLQAAWQWFTNLIAPVKATQDTLNRCRDT
                     GVMFGQALADALMLPLNAFNKLRSGIDWVLEKLGVINKESDTLDQTAARTHAATYGTG
                     GYIPATSSYAGYQAYQPVTAPAGRSYVDQSKNEYHISLTGGTAPGTQLDRQLQDALEK
                     YERDKRARARASMMHDG"
     gene            21861..22340
                     /locus_tag="ACHKIR_000516"
     CDS             21861..22340
                     /locus_tag="ACHKIR_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9988308.1"
                     /translation="MMLALGMFVFMRQTLPHQTMQRESDYRWPSNSRIGKRDAYQFLG
                     VGEENITLAGVLYPELTGGKLTMTTLRLMAEEGRAWPLLDGTGMIYGMYVISRVSETG
                     SIFFADGTPRKIDFTLSLTRVDESLAALYGDIGKQAESLIGKAGSMATRFTGMTGAG"
     gene            22340..23503
                     /locus_tag="ACHKIR_000517"
     CDS             22340..23503
                     /locus_tag="ACHKIR_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016157990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HFO9988309.1"
                     /translation="MLDALTFDAGSTLTPDYMLMLDSRDISGNISDRLMSMTLTDNRG
                     FEADQLDIELNDADGQVGLPVRGAVLTVYIGWKGFALVCKGKFTVDEVEHRGAPDVVT
                     IRARSADFRGTLNSRREGSWHDTTLGAIVEAIASRNRLEASVAPSLAGIKIPHIDQSQ
                     ESDAKFLTRLAERNGGEVSVKMGKLLFLKAGQGVTASGKKIPQVTITRSDGDRHHFAI
                     ADRGAYTGVTAKWLHTKDPKPQKQKVKLKRKKKEKHLRALEHPKAKPVTQKKAPKVPE
                     AREGEYMAGEADNVFALTTVYATKAQAMRAAQAKWDKLQRGVAEFSISLATGRADIYT
                     ETPVKVSGFKRVIDEQDWTITKVTHFLNNSGFTTSLELEVRLSDVEYETEDDE"
     gene            23585..23803
                     /gene="ogrK"
                     /locus_tag="ACHKIR_000518"
     CDS             23585..23803
                     /gene="ogrK"
                     /locus_tag="ACHKIR_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage transcriptional regulator OgrK"
                     /protein_id="HFO9988310.1"
                     /translation="MFHCPLCQHAAHARTSRYITDTTKERYHQCQNVNCSATFITYES
                     VQRYIVKPGEVHAVRPHPLPSGQQIMWM"
     gene            complement(24076..25437)
                     /gene="yegQ"
                     /locus_tag="ACHKIR_000519"
     CDS             complement(24076..25437)
                     /gene="yegQ"
                     /locus_tag="ACHKIR_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HFO9988311.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTTFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGELAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(25585..25917)
                     /gene="yegP"
                     /locus_tag="ACHKIR_000520"
     CDS             complement(25585..25917)
                     /gene="yegP"
                     /locus_tag="ACHKIR_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HFO9988312.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            complement(26108..26830)
                     /gene="baeR"
                     /locus_tag="ACHKIR_000521"
     CDS             complement(26108..26830)
                     /gene="baeR"
                     /locus_tag="ACHKIR_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HFO9988313.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLMIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLEALDEE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            complement(26827..28230)
                     /gene="baeS"
                     /locus_tag="ACHKIR_000522"
     CDS             complement(26827..28230)
                     /gene="baeS"
                     /locus_tag="ACHKIR_000522"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HFO9988314.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLITMHWAVRISFERGFIDYIK
                     HGNEQRLQMLGDALGEQYAQHGNWRFLRNNDRFVFQILRSLEHDNNEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPVPPDGTRRPIMVNGAEVGAVIASPVERLTRNTDINFD
                     RQQRQTSWLIVALSTLLAALATFPLARGLLAPVKRLVDGTHKLAAGDFTTRVAPTSED
                     ELGRLAEDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TLASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            complement(28227..29642)
                     /gene="mdtD"
                     /locus_tag="ACHKIR_000523"
     CDS             complement(28227..29642)
                     /gene="mdtD"
                     /locus_tag="ACHKIR_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9988315.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQIGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAITTLMLMPNYTMQTRCFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNCALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMAFIIALPAFVF
                     ARVPSDTHQNVAISRRKRSAQ"
     gene            complement(29643..32720)
                     /gene="mdtC"
                     /locus_tag="ACHKIR_000524"
     CDS             complement(29643..32720)
                     /gene="mdtC"
                     /locus_tag="ACHKIR_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HFO9988316.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRIGLNPQALFNQGVSLDDVRSAISNANVRKPQG
                     ALEDDTHRWQIQTNDELKTAAEYQPLIIHYNNGGALRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRGERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQAVTE"
     gene            complement(32721..35843)
                     /gene="mdtB"
                     /locus_tag="ACHKIR_000525"
     CDS             complement(32721..35843)
                     /gene="mdtB"
                     /locus_tag="ACHKIR_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HFO9988317.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGQGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFTNMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTENTPGLAALDTIRLTSSDGGVVPLSSIAKI
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALLIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            complement(35843..37090)
                     /gene="mdtA"
                     /locus_tag="ACHKIR_000526"
     CDS             complement(35843..37090)
                     /gene="mdtA"
                     /locus_tag="ACHKIR_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HFO9988318.1"
                     /translation="MKSSYKSRWVMVIVVVIAAIAAFWFWQGRNDSRSAAPGATKQAQ
                     QSPAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTITAANTVTVRSRVDGQLMALH
                     FQEGQQVKAGDLLAEIDPSQFKVALAQAQGQLAKDKATLANARRDLARYQQLVKTNLV
                     SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQIS
                     SGDTTGIVVITQTHPIDLLFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTL
                     LSLDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNE
                     GHFVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVE
                     AQSATTPEEKATSREYAKKGARS"
     gene            37369..37425
                     /locus_tag="ACHKIR_000527"
     CDS             37369..37425
                     /locus_tag="ACHKIR_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO9988319.1"
                     /translation="MMKILIIVVLLVVSYPAY"
     gene            37697..37753
                     /locus_tag="ACHKIR_000528"
     CDS             37697..37753
                     /locus_tag="ACHKIR_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO9988320.1"
                     /translation="MMKWIIIVVLLVISFPAY"
     gene            38025..38081
                     /locus_tag="ACHKIR_000529"
     CDS             38025..38081
                     /locus_tag="ACHKIR_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO9988321.1"
                     /translation="MMKLLIIVVLLVISWPAY"
     gene            38354..38413
                     /gene="ibsA"
                     /locus_tag="ACHKIR_000530"
     CDS             38354..38413
                     /gene="ibsA"
                     /locus_tag="ACHKIR_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HFO9988322.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            38634..39293
                     /gene="yegL"
                     /locus_tag="ACHKIR_000531"
     CDS             38634..39293
                     /gene="yegL"
                     /locus_tag="ACHKIR_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFO9988323.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMNGRPINELNAGL
                     VTFRDELLADPLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFQGEEDKKFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            39290..40051
                     /gene="pphC"
                     /locus_tag="ACHKIR_000532"
     CDS             39290..40051
                     /gene="pphC"
                     /locus_tag="ACHKIR_000532"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase PphC"
                     /protein_id="HFO9988324.1"
                     /translation="MSWRLVYASTVGTSHISADLPCQDACQMQVAWLNDQQPLLSVFV
                     ADGAGSASQGGEGAMLAVNEAMAYMSQKVQGGELGLNDVLATDIVLTIRQRLFAEAEA
                     KELAVRDFACTFLGLISSANGTLIMQIGDGGVVVDLGHGLQLPLTPMVGEYTNMTHFI
                     TDEDAVSRLETFTSTERAHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSGPAVNERTDDDKTLALALWLP"
     gene            40048..41987
                     /gene="yegI"
                     /locus_tag="ACHKIR_000533"
                     /pseudo
     CDS             40048..41987
                     /gene="yegI"
                     /locus_tag="ACHKIR_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
     gene            complement(42000..43352)
                     /gene="yegD"
                     /locus_tag="ACHKIR_000534"
     CDS             complement(42000..43352)
                     /gene="yegD"
                     /locus_tag="ACHKIR_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO9988325.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPQLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQGILERAAKRAGFKDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEESKIALSSVAETRASLPFISDELATLISQQGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALTEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            43486..44334
                     /gene="alkA"
                     /locus_tag="ACHKIR_000535"
     CDS             43486..44334
                     /gene="alkA"
                     /locus_tag="ACHKIR_000535"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HFO9988326.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVNGVETVADDYYARSLAVGEC
                     RGVVTAIPDIASHTLHINLSAGLEPVAAECLAKMSRLFDLQCNPQIVNGALGKLGAAR
                     PGLRLPGCVDAFEQGVRAILGQLVSVAMAAKLTARVAQLYGERLDDFPDYVCFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGTLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFPGMTPAQIRRYAERWKPLRSYALLHIWYTEG
                     WQPDEA"
     gene            complement(44443..47760)
                     /gene="dgcE"
                     /locus_tag="ACHKIR_000536"
     CDS             complement(44443..47760)
                     /gene="dgcE"
                     /locus_tag="ACHKIR_000536"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFO9988327.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLFSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLALGSAIVPPLLGGVLVILLTPGDDPLRAFLIWV
                     LSESIGALALVPLGLLFKPHYLLRHRNPRLLFESLLTLAITLTLSWLSMLYLPWPFTF
                     IIVLLMWSAVRLPRMEAFLIFLTTVMMVSLMMAADPSLLATPRTYLMSHMPWLPFLLI
                     LLPANIMTMVMYAFRAERKHISESETRFRNAMEYSAIGMALVGTEGQWLQSNKALCQF
                     LGYSQEELRGLTFQQLTWPEDLNKDLQQVEKLISGEINTYSMEKRYYNRNGDVVWALL
                     AVSLVRHTDGTPLYFIAQIEDINELKRTEQVNQQLMERITLANEAGGIGIWEWELKPN
                     IFSWDKRMFELYEIPPHIKPNWQVWYECVLPEDRQHAEKVIRDSLQSRSPFKLEFRIA
                     VKDGIRHIRALANRVLNKEGEVERLLGINMDMTEVKQLNEALFQEKERLHITLDSIGE
                     AVVCIDMAMKITFMNPVAEKMSGWTQEEALGVPLLTVLHITFGDNGPLMENIYSADTS
                     RSAIEQDVVLHCRSGGSYDVHYSITPLSTLDGSNIGSVLVIQDVTESRKMLRQLSYSA
                     SHDALTHLANRASFEKQLRILLQTVNSTHQRHALVFIDLDRFKAVNDSAGHAAGDALL
                     RELASLMLSMLRSSDVLARLGGDEFGLLLPDCNVESARFIATRIISAVNDYHFIWEGR
                     VHRVGASAGITLIDDNNHQAAEVMSQADIACYASKNGGRGRVTVYEPQQAAAHSERAV
                     MSLDEQWRMIKENQLMMIAHGVASPRIPEARNLWLISLKLWSCEGEIIDEQTFRRSFS
                     DPALSHALDRRVFHDFFQQAAKAIASKGLSIALPLSVAGLSSATLVNELLEQLENSPL
                     PPRLLHLIIPADAILDHAESVQKLRLAGCRIVFSQVGRDLQIFNSLKANMADYLLLDG
                     ELCANVQGNLMDEMLITIIQGHAQRLGMKTIAGPVVLPLVMDTLSGIGVDLIYGDVIA
                     DAQPLDLLVNSSYFAIN"
     gene            48078..48719
                     /gene="udk"
                     /locus_tag="ACHKIR_000537"
     CDS             48078..48719
                     /gene="udk"
                     /locus_tag="ACHKIR_000537"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFO9988328.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            48811..49392
                     /gene="dcd"
                     /locus_tag="ACHKIR_000538"
     CDS             48811..49392
                     /gene="dcd"
                     /locus_tag="ACHKIR_000538"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HFO9988329.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDESEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            49414..51267
                     /gene="asmA"
                     /locus_tag="ACHKIR_000539"
     CDS             49414..51267
                     /gene="asmA"
                     /locus_tag="ACHKIR_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HFO9988330.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQVNWQLQGADLPKQGIQGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLKETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVIGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(51320..51610)
                     /locus_tag="ACHKIR_000540"
     CDS             complement(51320..51610)
                     /locus_tag="ACHKIR_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO9988331.1"
                     /translation="MKIETFDSVWDAVSDTPEQAENMRIRAELVTIINNWIEQQGFSQ
                     AQAASALGVTQPRISELARGKIQIFSIDKLITMMAHAGLHIQRIEVQYPHAA"
     gene            complement(51600..51938)
                     /locus_tag="ACHKIR_000541"
     CDS             complement(51600..51938)
                     /locus_tag="ACHKIR_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO9988332.1"
                     /translation="MRKKLAFLDTSLDDLRAFPESSRQEIGYQLDRIQQGLNPYDWKP
                     FSTIGPGVREIRTRDADGIYRVMYVAKFEEAVYVLHCFQKKTQTTSQSNIDLAKRRYK
                     ELVQERKNEN"
     gene            complement(52013..53596)
                     /gene="yegH"
                     /locus_tag="ACHKIR_000542"
     CDS             complement(52013..53596)
                     /gene="yegH"
                     /locus_tag="ACHKIR_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFO9988333.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLHKDGEMEYEV"
     gene            complement(53797..53946)
                     /locus_tag="ACHKIR_000543"
     CDS             complement(53797..53946)
                     /locus_tag="ACHKIR_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9988334.1"
                     /translation="MVIEYWLSNNAGGSVTANGIAGKVTIRQLAIFKNYIKCHSIWFL
                     GVSLG"
     gene            54255..55394
                     /gene="wza"
                     /locus_tag="ACHKIR_000544"
     CDS             54255..55394
                     /gene="wza"
                     /locus_tag="ACHKIR_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HFO9988335.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            55400..55843
                     /gene="wzb"
                     /locus_tag="ACHKIR_000545"
     CDS             55400..55843
                     /gene="wzb"
                     /locus_tag="ACHKIR_000545"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HFO9988336.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFVAVYTLLERSARQWAQALNAEQV"
     gene            55846..58008
                     /gene="wzc"
                     /locus_tag="ACHKIR_000546"
     CDS             55846..58008
                     /gene="wzc"
                     /locus_tag="ACHKIR_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HFO9988337.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQSSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHASPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEDEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGEITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            58186..59025
                     /gene="wcaA"
                     /locus_tag="ACHKIR_000547"
     CDS             58186..59025
                     /gene="wcaA"
                     /locus_tag="ACHKIR_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HFO9988338.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            59028..59516
                     /gene="wcaB"
                     /locus_tag="ACHKIR_000548"
     CDS             59028..59516
                     /gene="wcaB"
                     /locus_tag="ACHKIR_000548"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HFO9988339.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRAAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            59513..60730
                     /gene="wcaC"
                     /locus_tag="ACHKIR_000549"
     CDS             59513..60730
                     /gene="wcaC"
                     /locus_tag="ACHKIR_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HFO9988340.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLLNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQRVAGKRQLFSEMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEDVLQLVQSSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            60705..61922
                     /gene="wcaD"
                     /locus_tag="ACHKIR_000550"
     CDS             60705..61922
                     /gene="wcaD"
                     /locus_tag="ACHKIR_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid polymerase WcaD"
                     /protein_id="HFO9988341.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDAGKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVVLAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            61933..62679
                     /gene="wcaE"
                     /locus_tag="ACHKIR_000551"
     CDS             61933..62679
                     /gene="wcaE"
                     /locus_tag="ACHKIR_000551"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HFO9988342.1"
                     /translation="MLLSIITVAFRNLAGIVKTHASLAHLAQADDISFEWIVVDGGSN
                     DGTREYLENLNGIYNLRFVSEPDNGIYDAMNKGIAMAHGKFALFLNSGDIFHQDAANF
                     VRKLKVQKDNMMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKS"
     gene            62695..63243
                     /gene="wcaF"
                     /locus_tag="ACHKIR_000552"
     CDS             62695..63243
                     /gene="wcaF"
                     /locus_tag="ACHKIR_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HFO9988343.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGNGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            63270..64391
                     /gene="gmd"
                     /locus_tag="ACHKIR_000553"
     CDS             63270..64391
                     /gene="gmd"
                     /locus_tag="ACHKIR_000553"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HFO9988344.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYDMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            64394..65359
                     /gene="fcl"
                     /locus_tag="ACHKIR_000554"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             64394..65359
                     /gene="fcl"
                     /locus_tag="ACHKIR_000554"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HFO9988345.1"
                     /translation="MSKQRIFIAGHRGMVGSAIRRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASESIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            65362..65841
                     /gene="gmm"
                     /locus_tag="ACHKIR_000555"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             65362..65841
                     /gene="gmm"
                     /locus_tag="ACHKIR_000555"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HFO9988346.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVVEEELLLPDEQHDDYRWLTPDALLASDNVHANSRAYFLAEKRAGVPGL"
     gene            65838..67061
                     /gene="wcaI"
                     /locus_tag="ACHKIR_000556"
     CDS             65838..67061
                     /gene="wcaI"
                     /locus_tag="ACHKIR_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HFO9988347.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWQPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAAENVIFFPNWSEIARFQHVANADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            67064..68500
                     /gene="cpsB"
                     /locus_tag="ACHKIR_000557"
     CDS             67064..68500
                     /gene="cpsB"
                     /locus_tag="ACHKIR_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HFO9988348.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRTAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAIDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            68693..70063
                     /gene="cpsG"
                     /locus_tag="ACHKIR_000558"
                     /gene_synonym="manB"
     CDS             68693..70063
                     /gene="cpsG"
                     /locus_tag="ACHKIR_000558"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HFO9988349.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDARLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     NNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLADPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            70118..71512
                     /gene="wcaJ"
                     /locus_tag="ACHKIR_000559"
     CDS             70118..71512
                     /gene="wcaJ"
                     /locus_tag="ACHKIR_000559"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HFO9988350.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRIEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            71514..72992
                     /gene="wzxC"
                     /locus_tag="ACHKIR_000560"
     CDS             71514..72992
                     /gene="wzxC"
                     /locus_tag="ACHKIR_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HFO9988351.1"
                     /translation="MNLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGIVVCVAVFLLSDTI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYRPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSVVGIINFPALLGLIVVSNNFVPLVFGEKWNSIIPVLQLLCVVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIATGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            73653..74933
                     /gene="wcaK"
                     /locus_tag="ACHKIR_000561"
     CDS             73653..74933
                     /gene="wcaK"
                     /locus_tag="ACHKIR_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HFO9988352.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHAEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMKQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRSNITTAKVEHGVDTAWLVDHHIEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPALNTRLNEAVSRERQTGMQMVQSVLERIGEVK"
     gene            74930..76150
                     /gene="wcaL"
                     /locus_tag="ACHKIR_000562"
     CDS             74930..76150
                     /gene="wcaL"
                     /locus_tag="ACHKIR_000562"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HFO9988353.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIIALQKGDTQN
                     THAAWTKYNLAARTRWLQDEPTGKVAKLRHRASQTLCGIHRKNTWQALNLKRYGAESR
                     NLILSAICGQVATPFHADVFIAHFGPAGVTAAKLRELGVIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGPWERRLRTLIEQYQ
                     LEDVIEMPGFKPSHEVKAMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLDTDELAPVIKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            76161..77555
                     /gene="wcaM"
                     /locus_tag="ACHKIR_000563"
     CDS             76161..77555
                     /gene="wcaM"
                     /locus_tag="ACHKIR_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HFO9988354.1"
                     /translation="MPFKKLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGLVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGEEGGSMHNITLDVRGSDCIIKGLAMSGFGPVTQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDRDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIK
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVALTNIEMKRASLELHNKPQHLFMRNIKV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAMNERGQSSVDIDRVNHHIV
                     NVEKINFRLPERRE"
     gene            77713..78708
                     /locus_tag="ACHKIR_000564"
     CDS             77713..78708
                     /locus_tag="ACHKIR_000564"
                     /EC_number="5.1.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,
                     octacis-undecaprenol 4-epimerase"
                     /protein_id="HFO9988355.1"
                     /translation="MNDNVLLIGASGFVGTRLLETAIADFNIKNLDKQQSHFYPEITQ
                     IGDVRDQQALDQALAGFDTVVLLAAEHRDDVSPTSLYYDVNVQGTRNVLTAMEKNGVK
                     NIIFTSSVAVYGLNKHNPDENHPHDPFNHYGKSKWQAEEVLREWYNKAPTERSLTIIR
                     PTVIFGERNRGNVYNLLKQIAGGKFMMVGAGTNYKSMAYVGNIVEFIKYKLKNVAAGY
                     EVYNYVDKPDLNMNQLVAEVEQSLNKKIPSMHLPYPLGMLGGYCFDILSKITGKKYAV
                     SSVRVKKFCATTQFDATKVHSSGFVAPYTLSQGLDRTLQYEFVHAKKDDITFVSE"
     gene            78951..79844
                     /gene="galF"
                     /locus_tag="ACHKIR_000565"
     CDS             78951..79844
                     /gene="galF"
                     /locus_tag="ACHKIR_000565"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HFO9988356.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            80216..81301
                     /gene="rfbB"
                     /locus_tag="ACHKIR_000566"
     CDS             80216..81301
                     /gene="rfbB"
                     /locus_tag="ACHKIR_000566"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFO9988357.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYIFEHADICDAAAMARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALDGDKKNSFRFHHISTDEVYGDLPHPDEVNNKEQLPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDAEKIGRELGWKPQETFESGIRKTVEWY
                     LSNTKWVDNVKSGAYQSWIEQNYEGRQ"
     gene            81301..82200
                     /gene="rfbD"
                     /locus_tag="ACHKIR_000567"
     CDS             81301..82200
                     /gene="rfbD"
                     /locus_tag="ACHKIR_000567"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFO9988358.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPEFAQLLNATSVESIAKAANEVGAWVIHY
                     STDYVFPGTGEIPWLETDATAPLNVYGETKLAGEKALQEQCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVALNKPEVAGLYHLVAS
                     GTTTWHDYAALVFEEARKAGIPLALNKLNAVATTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTVI"
     gene            82258..83136
                     /gene="rfbA"
                     /locus_tag="ACHKIR_000568"
     CDS             82258..83136
                     /gene="rfbA"
                     /locus_tag="ACHKIR_000568"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFO9988359.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFHQLLGDGGQWGLNLQYKVQPTPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDVAVNKESGATVFAYHVNDPERYGVVEFDKKGTAISL
                     EEKPLQPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMEQGGLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLRMIKGY"
     gene            83141..>83237
                     /locus_tag="ACHKIR_000569"
     CDS             83141..>83237
                     /locus_tag="ACHKIR_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HFO9988360.1"
                     /translation="MNVIKTEIPEVLIFEPKKFEDSRGYFFESFNQ"
BASE COUNT        20227 a        21160 c        22562 g        19288 t
ORIGIN      
        1 attcctaata ccctgaaagg ggtctcaatg ccaacttgcg cctgatttta cttatttttt
       61 gtcagtaagt aattagtttg gtgcatttat ttgcattcat ttttaaatgc cagttctggt
      121 atctaacatc agtgctggcg cattctaacc attactgctg cactgcattt aaagccctgc
      181 atgaagcggg cgggcgaggc ggggaaagca ctgcgcgctg gcggtggtgc tgattttatt
      241 ttttcagcgt ctgagcgcgt cgtgacggcg cttagtctgc ccgttgaggc gttggtgtgt
      301 ctgcggggtg ttttgtgcgg tggtgagcgt gtgagggcgt gatgacgggg tgtaaaaaag
      361 ccgcccgcag gcggcgatgt tcagtcgttg tcagtgtcca gtgagtagtt tttaaagcgg
      421 atgacctcct gaccgagcca gccgtttatc tcgcggatcc tgtcctgtaa cgggataagc
      481 tcattgcgga caaagacctt tgccactttc tcaatatcac ccagcgaccc gacgttctcc
      541 ggcttgccgc ccatcaactg aaaggggatg cggtgcgcgt ccagcaggtc agcggcgctg
      601 gcttttttga tattaaaaaa atcgtccttc gttgccactt cactgagcgg gataatttta
      661 atgccgtcgg ctttcccctg tggggcatag agaaacagat ttttaaagtt attgcggcct
      721 ttcgacttca ccatgttttc gcgaagcatt tcgatatcgt tgcgatcctg cacggcatcg
      781 gtgacgtaca tgatgtatcc ggcatgcgcg ccgttttcgt aatacttgcg gcggaacagc
      841 gtggccgact cattcagcca ggcagagtta agggcgctga gatattccgg caggccgtac
      901 agctcctgat taatatccgg ctccagcagg tgaaacacgg agccgggcgc gaaaggtgtc
      961 ggctcgttga aggacggcac ccaccagtaa acatcctcct caacaccacg gcgggtatat
     1021 tttgccggtg atgtttccag tctgatgacc ttaccggtgg tgctgtagcg cttttccaga
     1081 aacgcattac cgaacaccag aaaatccagc acaaagcggc tgaaatcctg ttgcgaaagc
     1141 cacggatgcg ggataaatgt tgaggccaga atattgcgtt tgacgtaaat cggtgagctg
     1201 tgatgcacgg cagcacgcag gctttttgcc agaccggtaa agctgaccgg tggctcatac
     1261 catctgccgt tactgatgca ctcgacgtaa tccagaatgt cacggcggtc gagtaccggc
     1321 accggctcac caaaggtgaa tgcctccatt ttcggggcgc tggcggtcat tgtttttgcc
     1381 gctggtcgcg gtgttttccc ttttttcttg ctcatcagta aaactccaga atggtggatg
     1441 tcagcggggt gctgataccg gcggtgagtg gctcatttaa cagggcgtgc atggtcgccc
     1501 aggcgaggtc ggcgtggctg gcttcctcgc tgcggctggc ctcataggtg gcgctgcgtc
     1561 cgctgctggt catggtcttg cggatagcca taaacgagct ggtgatgtcg gtggcgctga
     1621 cgtcatattc cagacagcca cggcggatga cgtcttttgc cttgagcacc attgcggttt
     1681 tcatttccgg cgtgtagcgg atgtcgcgtg cggcgggata gaacgagcgc acgagctgga
     1741 acacgccgac accgaggccg gtggcatcaa taccgatgta ttcgacgttg tatttctcgg
     1801 tgagtttgcg gatggattca gcctgagtgg caaagtccat gcctttccac tggtgacgct
     1861 caagtattct gaatttgcca ccggccacca ccggcggtgc cagtaccacg catccggcgc
     1921 tgtcgccacg gtgtgacggg tcgtaaccaa tccataccgg gcgggagccg aacggattcg
     1981 cggcaaacgg cgcatagtct tcccattctt ccagcgtgtc gaccatgcag cgttgcagct
     2041 cctcgaacgg gaacaccgac gccttgtcgt caacaaattc acacatgaac aggtttttaa
     2101 aatcgtcggc gctgttttcg cgtttgagct gctcaatgtc gaacagcgtg cagccgcctt
     2161 tcagggcgtc ctcaatggtg acaatctgcc gccactggcc gtccgcacag agaagtccac
     2221 cggcaagggc gttatgactg acgtcgattt ccacgcgttc ggcggcgctg gcgcgtcccc
     2281 ggttaaacag ttcacccgac cagaacgggt aggcgtcgtg cgccagcgtg gacggggtgg
     2341 agaaataggt cgaacgcagg tgactctgtg aggccatacc tgatgccacc ttacgcagta
     2401 cctgaaaatt cgggatccag aaaatctcgt cgacgtacag gtcgccgtta tggctctgtg
     2461 cggtgttgga gttggtgccg agaaaaatca gttttgcgcc gttattgccc aggacaatcg
     2521 ggtcaccggt caggtcaacg tcaaccagcc gggcaaaggc gatgatgtat tcgcggaaca
     2581 catacgcctg cgttttactg gccgacagaa aaatctggtt atgaccggtt ttcagggcgc
     2641 gcagcagcgc ctcgcgggaa aaataaaacg tcgcgccaat ctggcgggat ttcaggatat
     2701 cgcggatgcg gtgctcaagc ccggcgcgat accagtgcaa ctgatagtcg aaagactgct
     2761 caaagaaaat ctgctccagc ttttcgatgg cttcgtcact gaaaaaattc tttttcggtt
     2821 tgcgacgccc acctttgttg cggttagcaa tgttcggatt aaggtctgcc tcgttgccgg
     2881 tctggctgta acggttgacc cgtgccagtc gttcaatctg gcgtcccagc aggtcaattt
     2941 ccttgaagtc cccgccggtt ttctgcggtt tgatgatgag ctgggtcagc cgcgcttcca
     3001 gactcatttc gacacggctg atgggggcaa cgctgtccca gccgtcgcgc tgtttccagc
     3061 tctgcaccgt cgggcgtttc atctgcaaca tggcggcaat ctgcggcacg gaaaatccct
     3121 gccagtacag cagcgccgcc tgacgacgcg ggtcgtgtaa aagagtggtg tctgtggtga
     3181 tggtcatgaa tacctcgccg tgataaatac acggcaaggc tactgagtcg cgtcccgcga
     3241 ttcgctaagg tgctgttgtg tcagtgataa gccatccggg actgatggcg gaggatgcgc
     3301 atcgtcggga aactgatgcc gacatgtgac tcctctaatc actattcagg actcctgaca
     3361 atggcaaaaa aagtctcaaa attctttcgt atcggcgttg agggtgacac ctgtgacggg
     3421 cgtgtcatca gtgcgcagga tattcaggaa atggccgaaa cctttgaccc gcgtgtctat
     3481 ggttgccgta ttaacctgga acatctgcgc ggcatcctgc ctgacggtat ttttaaacgt
     3541 tatggtgatg tggtcgaact gaaggccgaa aagattgacg atgattcggc gctgaaaggc
     3601 aaatgggcgc tgtttgcgaa aatcaccccg accgatgacc ttatcgcgat gaacaaggcc
     3661 gcgcagaagg tctatacctc aatggaaatt cagccgaact ttgccaacac cggcaaatgt
     3721 tatctggtgg ggctggccgt caccgatgac ccggcaagcc tcggcacgga atacctggaa
     3781 ttctgccgca cggcaaaaca caaccctctg aaccgcttca aattaagccc tgaaaacctg
     3841 atttcagtgg caacgcctgt tgagctggaa tttgaagacc tgcctgaaac cgtgttcacc
     3901 gccctgaccg aaaaggtgaa gtccattttt ggccgcaaac aggccagcga tgatgcccgt
     3961 ctgaatgacg tgcatgacgc ggtgaccgct gttgctgaac atgtgcagga aaaactgagc
     4021 gccactgagc agcgcctcgc tgagatggaa accgcctttt ccgcacttaa gcaggaggtg
     4081 actgacaggg cggatgaaac cagccaggca ttcacccgcc tgaaaaacag tctcgaccac
     4141 accgaaagtc tgacccagca gcgccgcagc aaggccaccg gtggtggcgg tgacgccctg
     4201 atgacgaact gctgaccggc gtcagtcagt ccgggaaaac cttcacgatt aacccttaat
     4261 ttcaggaaaa actatgcgcc aggaaacccg ctttaaattt aatgcctacc tgtcccgtgt
     4321 tgccgaactg aacggcatcg acgccggtga tgtgtcgaaa aaattcaccg ttgaaccgtc
     4381 ggtcacccag accctgatga acaccatgca ggagtcctct gactttctga cccgcatcaa
     4441 cattgtgccg gtcagcgaaa tgaaagggga aaaaattggc atcggtgtca ccggctccat
     4501 tgccagcacc accgacaccg ccggtggcac cgagcgtcag ccgaaggact tctcgaagct
     4561 ggcgtccaac aagtacgaat gcgaccagat taacttcgat ttttatatcc gctacaaaac
     4621 gctggacctg tgggcgcgtt atcaggattt ccagctccgt atccgtaacg ccattatcaa
     4681 acgccagtcc cttgatttca tcatggccgg ttttaacggc gtgaagcgtg ccgaaacctc
     4741 tgaccgcagc agcaatccga tgctgcagga tgtggcagtc ggctggctgc agaaataccg
     4801 caatgaagct ccggcgcgcg tgatgagcaa ggtcactgac gaggaagggc acaccacctc
     4861 tgaggtcatc cgcgtgggca agggcggtga ttatgccagc cttgacgcac tggtgatgga
     4921 tgcgaccaat aacctgattg agccgtggta tcaggaagac cctgaccttg tggtgattgt
     4981 gggacgtcag ttactggcgg acaagtattt ccccatcgtt aacaaggagc aggacaacag
     5041 cgaaatgctg gccgctgacg tcatcatcag ccagaaacgc atcggtaacc tgccggcggt
     5101 acgcgtcccg tacttcccgg cggatgcgat gctcatcacg aagctggaaa acctgtccat
     5161 ctactacatg gatgacagcc atcgccgcgt gattgtggaa aacccgaaac tcgaccgcgt
     5221 ggagaactac gagtcaatga acattgatta cgtagtggaa gactacgccg ccggttgtct
     5281 ggtggaaaaa attaaggtcg gtgatttctc cacaccggct aaggcgaccg cagagccggg
     5341 agcgtaaccg atgacgagtc ccgcacagcg ccacatgatg cgggtctcgg cagcgatgac
     5401 cgcgcagcgg gaagccgccc cgctgcgaca tgcaactgtc tatgagcaga tgctggttaa
     5461 gctggccgca gaccagcgca cactgaaagc gatttattca aaagagctta aggccgcgaa
     5521 aaagcgcgag ctgctgccgt tctggttgcc gtgggtgaac ggtgtgctgg agcagggcaa
     5581 aggtgcacag gatgacattc tgatgacggt catgctgtgg cgtctggata ccggcgatat
     5641 tgccggtgcg ctggagattg cccgttatgc cctgaagtac ggtctgacca tgccgggtaa
     5701 acaccgccgc accccgccgt acatgttcac cgaggaggtc gcactcgcgg ccatgcgcgc
     5761 tcacgctgcc ggtgaatctg tggatatccg cctgctgacg gagacccttg aactgaccgc
     5821 cacggctgac atgcctgatg aagtgcgcgc aaagctgcac aaaatcaccg gtctgtttct
     5881 gcgtgatggt ggtgatgccg ccggtgcgct ggctcacctg caacgtgcga cacagctcga
     5941 ctgtcaggca ggcgtcaaaa aagagattga acgactggag cgggagctga aaccgaagcc
     6001 ggagccgcag cccaaagcgg ccacccgtgt cccgcgtaag acccggagcg tgacaccggc
     6061 aaaacgtgga cgcccgaaaa agaaagccag ttaacaaccg aatgcgcccc gcgccagggc
     6121 ggcacgccgg tcagtgacgg tgaattacct gacactgcac cggcgtccac cgcccgactt
     6181 ttcagaggta gtcatgatga cgctgattat tccgcgaaag gaggctcccg tgtccggtga
     6241 gggtacggtg gtcatcccgc aaccggcagg cgacgagccg gtgattaaaa acacgttctt
     6301 ttttcccgat atcgacccga agcgcgtccg ggaacgtatg cgccttgagc agaccgtcgc
     6361 ccccgcccgt ctgcgtgagg ccatcaagtc aggcatggct gaaacgaatg cggagctgta
     6421 cgagtaccgc gaacagaaaa ttgccgccgg ttttacgcgt ctggcggacg ttccggcgga
     6481 cgacatcgac ggtgaaagca tcaaagtttt ttactacgag cgcgccgtgt gtgcgatggc
     6541 gaccgcgtcg ctttatgagc gttatcgcgg cgtggatgcc agtgcgaaag gcgacaagaa
     6601 ggccgacagc attgacagca ccattgatga actgtggcgg gatatgcgct gggcggtggc
     6661 gcgtatccag gacaagccgc gctgcatcgt gagtcaaatc tgatgaagac ctttgcgcta
     6721 cagggcgaca cgctcgacgc catttgtgtc cggtattacg ggcgcactga gggcgtggtt
     6781 gagaccgtgc tcgccgcaaa tccgggactg gctgaactgg gtgcggtgct gccacacggc
     6841 accgccgtcg aactgcccga cgttcagacc gcgcccgtgg ctgaaactgt caatctgtgg
     6901 gagtaacgca tgacagcaga agaaaaaagc gtcctgtcgc ttttcatgat tggggtgctg
     6961 attgttgtcg gcaaggtgct tgccggtggt gaacccatca ccccgcgtct gtttatcggg
     7021 cgcatgttgc tcggcggttt tgtctcgatg gttgccggtg ttgttctggt gcagtttcct
     7081 gacctgtcac tgcctgcggt gtgcggcatc ggttccatgc tgggtatcgc cggttatcag
     7141 gtgattgaga ttgccattca gcgccgcttt aagggcaggg ggaaaccgta atgccggtaa
     7201 ttaacacgca tcagaatatc gccgcctttc tcgacatgct ggccgtgtcc gaagggacgg
     7261 cgaatcatcc gctgacgaaa aaccggggct atgacgtgat agtcaccgga ctggacggaa
     7321 agccggaaat tttcaccgac tacagtgatc acccgttcgc gcatggccga ccggcgaagg
     7381 tgtttaaccg tcgcggtgaa aaatccacgg cctccggtcg ctatcagcag ctttacctgt
     7441 tctggccgca ctaccgcaaa cagcttgccc tgccggattt cagtccgttg tcacaggaca
     7501 ggctcgccat tcagttgatc cgcgaacgcg gtgcactgga tgacatccgg gcgggacgca
     7561 ttgagcgcgc catttcacgc tgtcgcaata tctgggcgtc cctgccgggt gccggttacg
     7621 gtcagcgtga gcattcactg gaaaaactgg tcaccgtctg gcgtaccgcc ggcggcgtac
     7681 cggcttaaac ggagtaaaca ccatgaagaa attatccctt tcactgatgc tgaacgtgtc
     7741 gctggcgctg atgctggcac tgtccctgat ttacccgcag agcgtggccg tcagttttgt
     7801 cgccacctgg gcgattctgg cgacggttat ctgtgtggtt gccggtggtg tcggcgtgta
     7861 tgccacggag tatgtgctgg aactctacgg gcgggagctg ccgccggaat cgctggccgt
     7921 gaagattgtc acgtcgctgt ttttgcagcc ggtgccgtgg cgcagacggg cggcggctct
     7981 ggtggtgatg gtggcgacgt ttatctcgct ggtcgctgcc gggtggattt ttaccgcgct
     8041 gatttatctt gtggcgtcgc tgtttttccg gctgatacgt aaagcctgtc gtcagcgtct
     8101 tgaggggcgg gaactatgtc aaagctgatg attgtgctgg tcgtgttgtt atcgctggcg
     8161 gtggccggtc tgtttctggt gaaacacaaa aatgccagcc tgcgcgcctc gctggacagg
     8221 gcgaataacg tcgccagtga acagcagacg accatcacca tgctgaaaaa tcagcttcat
     8281 gttgccatca ccagggcaga caaaaacgag ctggcgcagg tggcactgcg tcaggaactg
     8341 gagaacgccg cgaagcgtga agcacagcgc gagaaaacca tcacgaggtt actcaatgaa
     8401 aacgaagatt ttcgccgctg gtacggtgct gacctgcctg atgctgtgcg ccggttgcac
     8461 cagcgcccgg cctgcgcaga cgccagtgat tgtccacaac gcctgcccga aagtgagtct
     8521 ttgcccgatg ccgggcagtg acccgcagaa gaacggcgat ttaagtgccg atatccggct
     8581 gcttgagaac gcgctggcac gctgtgccag ccaggtaaaa atgattaaac actgtcagga
     8641 cgaaaacgat gctcaaaccc gacagcctgc gcagggcgct gactgatgcc gtcacggtgc
     8701 tgaaaaccag tcccgagatg ctgcggatat tcgtggataa cgggagtatt gcctccacac
     8761 tggcgacgtc tatgtcattc gaaaagcgtt acacgctcaa tgtgattgtg accgacttta
     8821 ccggtgattt tgacctgctc attgtgccgg tgctggcgtg gctgcgggaa aatcagcccg
     8881 acatcatgac caccgacgaa ggccagaaaa agggcttcac gttttatgca gacatcaaca
     8941 atgacagcag ctttgatatc agcatcagcc tgatgctgac cgagcgcacg ctggtcagtg
     9001 aggttgacgg cgcgctgcat gtgaagaata tcccggaacc tccgccgccg gagccggtta
     9061 cccgcccgat ggagctgtat atcaatggcg aactggtgag caagtgggat gaatgagttt
     9121 aagcgttttg aagaccggct gaccggactg attgagtcgc tgtcaccgtc agggcgtcga
     9181 cgactgagta ccgaactggc gaaacgtctg cgtcagagtc agcagcgtcg ggtgatggca
     9241 cagaaagccc cggacggcac accctacgcg ccacgccagc agcagagcgc cagaaaaaag
     9301 accggtcgtg ttaagcgaaa aatgtttgcg aaacttatca ccagtcgctt tttgcatatc
     9361 cgcgccagcc cggaacaggc atcaatggaa ttttacggcg ggaagtcacc aaaaatcgcc
     9421 agcgtgcatc agttcggcct gtcggaagaa aaccggaaag acggtaagaa aattgattat
     9481 ccggcgcgtc ccctgctcgg ctttaccggt gaggatgtgc agatgattga agagactatc
     9541 ctggctcacc ttgagcgtta gttttatcca ggcagaggct gatgcgcaat taaacattga
     9601 gcggccgtgc tggtcgctca atgtttagag gtttatgagt ggtttttatt tgatgctttg
     9661 tattctaaaa ccttcttatt ggcgtaaagg aattttgtat atgacaggaa tataagcagg
     9721 cctgaaatga aatagacgag ggatagtatt aataatgctt ttttgtggct gttattatct
     9781 ttaatctcct gacttaacca ttcggagtcc tcctcgttta gctgtaagag cttattgcag
     9841 gcgatttcag gaagtgtgtc ttttataaat acgttttgca gggtcttgca atcggcaagg
     9901 ctataagttt tattaagttc aactgtttta tttttgaagg ataaaagaac tttgtcacta
     9961 taaacatagt acatcatatt tttatatggt atgcctatgg catctcttac tatagcggat
    10021 tgtttgttgt gtatataaca tgcgaagaga atataaataa tactggccag aattacaatt
    10081 attgttttaa ttatgtgtgg tggttttgtt atgtcacccc agaagcgagt aaggaaaaaa
    10141 tacgatgttt ttagttttcc atcaatcagc ccctgctgta tcattctcac atcttcgatg
    10201 cctgatacat tgattccgtt aattgtttta aatagttgaa tgtcgcgcca ttttttgtcg
    10261 agcattttta actttctgtc tgaatactca agattgaaat gatgtgcaat aaacctcata
    10321 agattacttt taccaaaggt aacaaatgtt aatgctatta ataaaaatag atacaaagag
    10381 ataaaccacc acgcattaat cacattatca ctgaacatta cgctctcctc gaatgttgta
    10441 tggtcgttct acaaatgaat ccagatagca taacttttat atattgtgca atctcacacg
    10501 catgaacact ctcgcaaata ttcaggaact cgcgcgcgca ctgcgcaaca tgattcgtac
    10561 cggccttgtc gtcgaaaccg accttaacgc cggtcgctgc cgtgtgcaga ccggcggcat
    10621 gtgcaccgac tggcttcagt ggctgaccca tcgcgccggg cgttcgcgca catggtgggc
    10681 accttccgtg ggggaacagg tgctgattct ggccgtgggt ggtgaacttg acacggcgtt
    10741 cgttctgccg gggatttatt ccggcgataa ccccgcgccg tctgcgtcgg cggatgccct
    10801 gcatatccgt ttccctgacg gggcggtgat tgagtatgaa cccgaaacca gtgcactcac
    10861 ggtaagcgga attaaaacgg ccagcgtgac ggcttctgat tctgttactg ccacggtgcc
    10921 ggtggtcatg gtgaaagcgt caactcgcat caccctggac acaccggagg tggtctgcac
    10981 caacaggctg attactagca cgctggaagt acagaagggc gggacgatgc gtggcaacat
    11041 tgaacacacc ggcggtgaac tctcatcaaa cggtaaggta ctgcataccc acaaacaccc
    11101 cggcgacagc ggcggcacaa ccgggagtcc tttatgacag ctcgttatct cggaatgaat
    11161 cgcagtaatg gcctgactgt cactgacctt gagcatatca gccagagtat cggcgatatc
    11221 ctgcgtacac cggtcggctc acgggtgatg cgtcgtgatt acggctcgtt gctggcatca
    11281 atgattgacc agccgcagac cccggcgctt gagttgcaga ttaaggtcgc ctgttacatg
    11341 gcggtgctga aatgggaacc ccgcgtcacg ctgtcatctg tcaccacggc gcgcagtttt
    11401 gacgggcgaa tgacggtcac gttaaccggc cagcacaacg acaccggcca gccactttcg
    11461 ttaaccatcc ctgtgagttg aaaccatgcc gattatcgac ctgaaccagc tacccgcacc
    11521 ggatgtggtc gaggagctgg actttgaaac cattctcgcc gaacgcaagg cgacactgat
    11581 ttccctttac ccggaagacc agcaggaggc ggtcgcccgt accctgacgc tggaatctga
    11641 gcctctcgtc aaactgctgg aggaaaatgc ttatcgtgag cttctctggc gtcagcgtgt
    11701 gaatgaggcc gcacgggcgg tgatgctggc ctgtgccgcg ggtaatgacc ttgatgtgat
    11761 tggtgccaat tacaacacca cgcgtctgac tatcaccccg gcagatgatt cgaccatccc
    11821 gccgacaccg gccgtgatgg aatctgacac cgattatcgt ctgcgtattc agcaggcctt
    11881 tgaaggttta agcgtcgccg ggtcggtggg tgcctatcag tatcatggtc gcagtgctga
    11941 cgggcgtgtc gcggatatct ctgtcaccag tccgtctccg gcctgcgtca ccatctctgt
    12001 gctgtcacgt gaaaataacg gtgtcgcatc cgaagacctg ctggcggtgg tgcgtaacgc
    12061 ccttaatggc gaggacgtca ggccggtggc cgaccgcgtg accgtgcagt ctgccgccat
    12121 cgttgaatac cagataaacg ccacgcttta cctttaccct ggtcccgaaa gcgaacccat
    12181 acgcgctgcc gccgtgaaaa aactggaagc gtacatcacg gcacagcacc ggctggggcg
    12241 cgacatccgt ctgtctgcca tttatgccgc tttgcatgtg gaaggcgtgc agcgtgtcga
    12301 gctggccgca ccactggccg acatcgtgct caacagtacg caggcgtctt tctgtaccga
    12361 ataccgcgtc gtgaccggag gctcggatga gtgattcgcg actgttgccg accggctcat
    12421 caccgcttga ggtcgccgcc gcaaaagcct gtgcggaaat tgaaaaaaca ccggtcagta
    12481 ttcgtgagct gtggaacccg gactcctgtc cggcaaatct gctgccgtgg ctggcgtggg
    12541 cgttttcggt cgacagatgg gatgaaaagt ggccggaagc gacaaaacgc gctgttatcc
    12601 gcgatgccta tttcatccac tgtcataaag gcactatagg tgcaatccgg cgtgtggtgg
    12661 agccgctggg ctatctcatc aacgtgacgg agtggtggga aaccagtgac ccgccgggca
    12721 ccttccggct tgatattggt gtactggaaa gtggcatcac agaggcaatg tatcaggaaa
    12781 tggaacggct gattgctgat gccaaacctg caagccgcca ccttattggc ctgaacatta
    12841 cccgggacat tcccggctac ctgttcgccg gtggtgtggc ttacgacggc gatgtaatta
    12901 cggtttaccc cggataagtg aggaataatg agcacaaaat tcaaaaccgt tatcaccact
    12961 gccggtgcag caaagctggc agcggcaacc gcaccgggag ggcggaaggt caacattacc
    13021 acgatggccg tcggggatgg cggtggtaaa ttgcctgtcc cggatgccgg acagaccggg
    13081 cttatccacg aagtctggcg acatacgctg aacaaaatca gccaggacaa acgaaacagt
    13141 aattatatta tcgcagagct ggttattccg ccggaggtgg gcggtttctg gatgcgtgag
    13201 cttggcctgt acgatgatgc gggaacgtta attgccgtgg cgaacatggc cgaaagttat
    13261 aagccagctc ttgccgaagg ctcagggcgt tcgcagacct gtcgcatggt catcatcgtc
    13321 agcagtgtgg cctcggtgga gctgaccatt gacaccacaa cggtgatggc gacacaggat
    13381 tacgttgatg acaaaattgc agagcacgaa cagtcacgac gtcacccgga cgcctcgctg
    13441 accgccaaag gttttactca gttaagcagt gcgaccaaca gcacgtctga aacactggcc
    13501 gcaacgccga aagcggtaaa ggccgcgtat gaccttgcta acgggaaata taccgcacag
    13561 gacgctacca cagcgcgaaa aggtcttgtc cagctaagta gtgcgaccaa cagcatgtct
    13621 gaaacgctcg ccgcaacacc aaaagccgtt aagacggtaa tggatgaaac gaacaagaaa
    13681 gcgccattaa acagccctgc actgaccgga acgccaacga cgccaactgc gcgacaggga
    13741 acgaataata ctcagatcgc aaacacggct ttcgttatgg ccgcgattgc cgcccttgta
    13801 gactcgtcgc ctgacgcact gaatacgctg aacgagctgg cggcggcgct gggcaatgac
    13861 ccgaattttg ctaccaccat gactaatgcg cttgcgggta agcaaccgaa agatgccact
    13921 ttgacggcgc tggcggggct tgctactgcg gcagacaggt ttccgtattt tacggggaat
    13981 gatgttgcca gcctggcgac cctgacaaaa gtcgggcggg atattctggc taaatcgacc
    14041 gttgccgccg ttatcgaata tctcggttta caggaaacgg taaaccgagc cgggaacgcc
    14101 gtgcaaaaaa atggcgatac cttgtccggc gggcttactt ttgaaaacga ctcaatcctt
    14161 gcctggattc gaaatactga ctgggcaaag attggattta aaaatgatgc cgacagcgat
    14221 actgattcat atatgtggtt tgaaacaggt gacaacggca atgaatattt caaatggaga
    14281 agtcgccaga gcaccacaac aaaagacctg atgaatctta aatgggatgc tctgtatgtt
    14341 cttgttaaag cccttttcag tagtgaagta aaaatatcta cagtcaatgc gctgaggata
    14401 tttaattcat cttttggtgc tatttttcgc cgttctgaag aaaacctgta tatcattcct
    14461 acacgagaaa atgagggtga aaatggagat attgggtcat taaggccatt cggcatcaac
    14521 ttaagaacag gagttgtgtc tgttggtaat ggtgccagga ttgatggcgg gctggcactt
    14581 ggcacgaata acgcgttggg tgggaactct attgttcttg gtgataacga caccggattt
    14641 aaacaaaatg gcgatggtaa tctggatgtt tatgctaata acgtccatgt tatgcgcttt
    14701 gtttccggaa gcattcaaag taataagacc ataaatatta cggggcgtgt taatccctcg
    14761 gattacggta actttgattc ccgctatgtg agagatatca gacttggcac acgtgttgtc
    14821 cagaccatgc agaaaggggt gatgtatgag aaagcagggc acgtaattac cgggcttggt
    14881 attgtcggtg aagtcgatgg tgatgacccc gcagtattca ggccaataca aaaatacatc
    14941 aatggcacat ggtataacgt cgcacaggtg taatttatgc agcatttaaa aaatattact
    15001 gcgggtaatc caaaaactgt tgcccaatat caactgacaa aaaattttga tgttatctgg
    15061 ttatggtccg aagagggaaa aaactggtat gaggaagtaa gtaattttca ggaagacacg
    15121 ataaagattg tttacgacga gaataatata attgtcggca tcaccagaga tgcttcaacg
    15181 cttaaccctg aaggtttcag cgttgtcgag gttcctgata ttacctccaa ccgacgtgct
    15241 gatgactcag gtaaatggat gtttaaggat ggtgccgtga ttaagcggat ttatacggca
    15301 gatgaacagg agcaacaggc agaatcacaa aaggcagctt tactttccga agctgaatcc
    15361 gtgattttgc cgctggaacg cgctgtcagg ctgaatatgg cgacggatga ggaacgcagc
    15421 cgactggaag catgggaacg ctacagcgtt ctggtcagtc gtgtggatcc tgcaaatccc
    15481 gaatggccgg aaatgccgca ataagttgta tgatctctgg tgtgagctta catatctata
    15541 gcacagagta aagcctaatc tgacagtccg ctatgtgcca aaagcggacg ttaagcttac
    15601 caactcatat tttaaggtta taggtgcgta taaacgaagt gccgcgctta ggagtagtga
    15661 atttacagac ttacaaaatc atttatttca ggccaattta tgtaatggca taatcaggtt
    15721 ggtgaaagag agggcggaaa tggataaaaa atttaaaaat cagatagtag atagcgatat
    15781 tgcacgttta ttgcttaaac acaggaaggc tcgcaatttg actgtaacag agcttgctca
    15841 acgatcgggc gtcagtcagg ccatgattag taagattgaa cgaggcatct ccagccctag
    15901 tgcaactatc cttagccgcc tggccaacgc catgaatgta acgctatcta aactctttgc
    15961 agagcttgag caacatcaga attccctggt tcgttttgct catcagcaac agcactggac
    16021 ggatgagcaa tcaggcatca cccgttggtc tttgtctcct gcgggggcat gcccggaact
    16081 gatcaaagtt gaaatccctg caatgggtga gctgacgata cccgcctccg caaatgagca
    16141 tctttccggg caaacgcttt ggatgatgag tggcagcctt gattttcgag tcaaaaatca
    16201 gacccatcat ttacgtgccg gtgactgtct tgcccttacc tttgcaagcg agtaccaaat
    16261 gagcaatcca gatagtgagc agagctgttc ttacatcgtg gcttttagcc aaaaaaatca
    16321 atgatatctt ctctgccaca ttggttacat gttattggcc attctcagat gcaacaaggg
    16381 gtatggcctt ccctgattat ccagatcgga acgccctgat acctgaaaac ccatatgctg
    16441 ataaaaccct acagcctgct ggttttgttc atttacatca acgcttagct ctggatgcag
    16501 cgccagtgca tgagatatca aacgttttcc gacgccaagt ccgcgagcag agcctgctac
    16561 aaaaagtgcc tccagatgcc cctcatgcaa aaacataaat cctagcggtt ggtcatcctg
    16621 atttgttgca acccatacag gagtttctga gaaaaagccg ataacttctt tttcgatttc
    16681 ttgtcggtca tgacctgtaa gaaagtcgtg ggtagcatcc actgaactct tccagatttg
    16741 tatgatctct gatgcttcag atggtcgtga cattctgata tctaacattt ttcaacctta
    16801 gagagaactt tattttatgt aataacgaac gaaaagagct tactttaatt tactatagtg
    16861 aaattatatt cacaatagtg tttgcggttt ctatgcctta tcaacgtgat gagaagtgct
    16921 aagttagtct gatcctcttt atcatcatca acgacaatgt tcctgagctt tatcacattg
    16981 aacttccgct tctcgctcaa agcagactgt cagatttgat agcgttttgg ctatgtaaat
    17041 tgtcagtcgg gaaataagtg agtacaaatc atgacaggcg ggcggattgt ccgccttttc
    17101 tttatctgtt gtttcatcca ctgaccagcc aggtcaaata gcgtctcatg ctctgcacaa
    17161 cagaaaatac cactcaccca ttaaccacgg agttagacgg atgagtgact atcatcacgg
    17221 cgtgcaggtg ctggagatta acgacggcac ccgcgtcatt tccaccgtat ccactgccat
    17281 tgtcggcatg gtctgcacgg ccagcgatgc ggatgcggaa acattccccc tcaataaacc
    17341 tgtgctgatt accaatgtgc agagcgcaat tgcaaaggcc ggtaaaaaag gcacgctggc
    17401 ggcgtcgtta caggccatcg ctgaccagtc aaaaccggtc accgttgttg tgcgcgtgga
    17461 agacggcacc ggcgacgacg aggaaacgaa actcgcgcag actgtttcca atattatcgg
    17521 caccaccgac gaaaacggtc agtacaccgg actgaaagcc ctgatggggg ctgagtcggt
    17581 taccggcgtt aaaccgcgca ttctcggcgt accgggactg gacaccaaag aggtcgccgt
    17641 cgcactggca tcggtatgcc aggaactgaa tgccttcggg tatatcagcg catggggttg
    17701 taaaaccatt tccgaggtga aagcctaccg ccagaatttc agccagcgtg agctgatggt
    17761 tatctggccg gatttcctcg catgggatac ggttaccagt accaccgcca ccgcgtatgc
    17821 caccgctcgt gcgctgggtc tgcgcgctaa aatcgaccag gagcagggct ggcataaaac
    17881 gctgtccaac gtcggggtga acggtgttac cggcatcagt gcatccgtct tctgggattt
    17941 gcagaagcca ggcactgatg ctgacctgct gaacgaggca ggcgtcacaa cgctggttcg
    18001 ccgcgacggt ttccgtttct ggggtaaccg tacctgctct gatgacccgc tgttcctctt
    18061 tgaaagctac acacgcaccg cgcaggtact ggccgacacg atggctgagg cgcacatgtg
    18121 ggctattgat aagcccatca ccgcaacgct gattcgcgac atcattgacg gcatcaatgc
    18181 caaattccgt gaactgaaaa acaacggtta tatcgtggat ggcacatgct ggttcagcga
    18241 agaatccaac gatgcggaaa ccctcaaggc cggaaaactg tatatcgact acgactatac
    18301 cccggtgcct cctctcgaaa acctgaccct gcgccagcgt attacttcca gatacctggc
    18361 aagtctggtc acttcggtta acagcaatta aggagcctga ccgatggcaa tgccgcgcaa
    18421 actcaagtta atgaacgtct ttctgaacgg ctacagctat cagggcgttg caaagtccgt
    18481 cacgctgcca aaactgaccc gtaagctcga aaactatcgc ggtgcgggga tgaacggcag
    18541 cgcaccggta gacctcggcc ttgatgatga tgctctgtca atggagtggt cgctcggtgg
    18601 attcccggat tcggttatct gggagcttta cgctgcaacc agtgctgatg ccgtaccgat
    18661 tcgttttgct ggttcttacc agcgcgacga taccggcgaa acggtggccg tcgaagtggt
    18721 catgcgtggg cgtcagaaag aagtcgatac cggagagggt aaacagggag aggataccga
    18781 atcgaaactc ccggtgattt gtacttattt caagttgacg atggacggta aggagctggt
    18841 cgaaatcgac accatcaaca tgattgagaa ggtgaacggc gtcgaccggc tggagcaaca
    18901 ccgccgcaat atcggcctgt gattttcatc cggtcagcct ggctgaccgg ttaaccccga
    18961 ttcagaagtg agaaaaccat gaacaaagaa aatgtgatta ccctggacaa tccggtcaag
    19021 cgtggtgagc aggttatcga acaggtcacg ctgatgaaac ctaatgccgg gacgctgcgc
    19081 ggtgtcagtc tggctgcggt cgcaaactcc gaagtcgatg cactgattaa ggtgctgccg
    19141 cgcatgacgg caccgatgct gaccgagcag gaggtcgccg cgctggaact gcctgacctt
    19201 gtggcgctgg ccggtaaggt ggtcggtttt ttgtctccga actcggtgca gtgacgtttc
    19261 cgaaaaatct gtcggtcgat gacctgatgg cagatgtggc agtgatattt cactggccgc
    19321 catcagaact gtatcccatg agcctgaccg aactcatcac atggcgcgaa aaggcgctcc
    19381 ggcgaagcgg aagcacgaat gagtaacaat gtaaaattac aggtattgct cagggctgtt
    19441 gaccaggcat cccgcccgtt taaatccatc cgtacagcga gtaagtcgct gtcgggggat
    19501 atccgggaaa cacaaaaatc actgcgcgag ctgaacggtc acgcatcccg tattgaggga
    19561 tttcgcaaga ccagcgcaca gcttgccgtg actggtcatg cacttgaaaa ggcacggcag
    19621 gaagccgaag cacttgccac acagtttaaa aacactgaac gtccgacccg tgctcaggcg
    19681 aaagtgctgg aatccgcaaa gcgtgcggcg gaggacttac aggcgaaata taaccgcctg
    19741 acggattccg ttaagcgcca gcagcgggaa ctggccgctg tgggaattaa tacccgcaat
    19801 cttgcacatg atgagcaggg actgaaaaac cgtatcagtg aaaccaccgc acagcttaac
    19861 cggcagcgtg acgcgctggc gcgtgtcagt gcgcaacagg caaaacttaa tgcagtaaaa
    19921 cagcgttatc aggccggaaa ggaactggcc ggaaatatgg cctcagtggg cgctgccggt
    19981 gtggggattg cggcggcgga aacgatggcc ggagttaagt tgctgatgtc cggttatgag
    20041 tttgcgcaga aaaactcaga attgcaggcc gtgctgggtg tggcaaaaga ctccgccgaa
    20101 atgaccgcac tacgcaaaca ggcgcgccag ctcggcgaca ataccgccgc ctcggcggat
    20161 gatgcggccg gtgcacagat aatcatcgcg aaagcgggtg gggatgttga tgccattcag
    20221 gcggcaacgc cggtcacgct gaatatggcg ctggcgaacc gccgcacgat ggaagaaaac
    20281 gccgccctgc tgatggggat gaaatccgcc tttcagcttt caaacgataa ggtcgctcat
    20341 atcggggatg ttctctccat gacgatgaac aaaaccgccg ccgattttga cggcatgagc
    20401 gatgcgctga cctatgccgc acctgtggca aaaaatgccg gtgtcagcat tgaagaaacc
    20461 gccgcaatgg tcggggcgct gcatgatgca aaaatcacag gctcaatggc ggggacggga
    20521 agccgtgccg tgttaagccg cctgcaggca ccgacgggaa aagcatggga tgcactcaaa
    20581 gagcttggag tgaaaacctc agacagcaag ggaaacaccc gaccagtatt taccattctg
    20641 aaagaaatgc aggccagttt tgagaaaaac cggctcggta ctgcccagca ggctgaatac
    20701 atgaaaacca ttttcgggga ggaggccagc tcagccgccg ccgtgctgat gactgtcgcc
    20761 tcaaccggaa agctggacaa actgaccgct gcgtttaaag cctcggacgg gaagacctca
    20821 gagctggtaa atattatgca ggacaatctc ggcggtgact ttaaggagtt tcagtccgct
    20881 tatgaggcgg tggggactga cctgtttgac cagcaggaag gcgcactgcg taagctcacg
    20941 cagacggcca caaagtatgt gttaaaactc gacggctgga tacagaaaaa caaatcactg
    21001 gcgtcaacca tcggcatcat tgccggcggt gcactggtgc tgactggcat catcggtgcc
    21061 attggccttg tagcctggcc ggttatcacc ggcatcaatg ccattatcgc ggcagcaggc
    21121 gcaatggggg caatcttcac gacggttggt agtgccgtta tgacggccat cggtgcgatt
    21181 agctggccgg ttgtggccgt ggtggctgcc attgtcgccg gggcgttgct tatccgtaaa
    21241 tactgggagc ctgtcagcgc attctttggc ggtgtggttg aagggctgaa agcggcattt
    21301 gcgccggtgg gggagctgtt cacgccactt aagccggtgt ttgactggct gggcgaaaag
    21361 ttacaggccg cgtggcagtg gtttacaaac ctgattgctc cggttaaagc cacacaggac
    21421 accctgaacc gttgccgtga cacgggcgtc atgttcgggc aggcactggc tgacgcgctg
    21481 atgctgccgc ttaatgcgtt caacaaactg cgcagcggta ttgactgggt actggaaaaa
    21541 ctcggtgtta tcaacaaaga gtcagacaca cttgaccaga ccgccgccag aactcatgcc
    21601 gccacgtatg gcaccggtgg ttatattccg gcgaccagct cttatgcagg ctatcaggct
    21661 tatcagccgg tcacggcacc ggctggccgc tcttatgtgg accagagtaa aaacgaatat
    21721 cacatcagcc tgacgggtgg tactgcgccg gggacacagc ttgaccgcca gttacaggat
    21781 gcgctcgaaa aatacgagcg ggataaacgt gcgcgcgccc gtgccagcat gatgcatgac
    21841 ggttaaggag gtgacgaaaa atgatgctcg cgttaggtat gtttgttttt atgcgccaga
    21901 cgctgccaca ccagaccatg cagcgtgaat cagattatcg ctggccgtca aattcccgta
    21961 tcggtaaacg ggacgcctac cagtttctcg gtgtgggtga ggaaaacatc acgcttgccg
    22021 gtgtgcttta tccagaactg accggcggca agctgacgat gaccacgctc aggctgatgg
    22081 cagaggaagg ccgggcgtgg ccgttgctgg atggcaccgg catgatttac ggcatgtatg
    22141 tcatcagcag ggtgagtgaa acaggaagta ttttctttgc agacggcaca ccccgaaaaa
    22201 ttgattttac gctgtcgctc acccgcgttg atgaatcact ggccgcgctt tatggcgata
    22261 tcggtaaaca ggcggaatcg ctcatcggta aggctggcag tatggcgacc agattcacgg
    22321 gtatgacggg ggcgggataa tgctggatgc actgacattt gatgcaggca gtacgctgac
    22381 gccggattac atgctgatgc tcgacagcag ggatatttcc ggcaatatca gcgaccgact
    22441 gatgagcatg accctgacgg ataaccgggg ctttgaggct gaccagcttg atattgaact
    22501 gaacgatgcc gacgggcagg tcgggctgcc ggttcgtggc gctgtcctga cggtgtatat
    22561 cggctggaaa ggttttgccc tggtatgcaa agggaaattt accgttgatg aggttgaaca
    22621 ccggggcgca ccggatgtgg tcaccatccg cgcccggagt gcagattttc gcgggacgct
    22681 caattcccgc cgtgaaggct cctggcatga caccacgctc ggtgcgattg ttgaggcgat
    22741 agcctcccgt aacaggctgg aagccagtgt cgctccgtca ctggccggaa ttaaaatccc
    22801 gcacatcgac cagtcgcagg agtctgatgc gaaattcctg acccgtcttg cagaacgcaa
    22861 cggcggtgag gtgtcggtaa aaatgggaaa actgttgttt ctcaaagcgg ggcagggggt
    22921 gacggccagc ggtaaaaaaa tcccgcaggt caccataacc cgcagcgacg gcgaccgcca
    22981 tcattttgcg attgctgacc gtggagccta taccggcgta acggcaaagt ggttacacac
    23041 caaagacccg aagccacaaa agcagaaggt aaaactgaaa cgcaaaaaga aagagaaaca
    23101 cctgcgcgca ctggagcacc cgaaagcgaa accagtcacg cagaagaaag cgccaaaagt
    23161 accggaagcg cgcgaaggtg aatacatggc tggtgaggct gacaacgttt ttgccctgac
    23221 cacggtatat gccacgaaag cacaggccat gcgcgccgct caggcgaagt gggataagct
    23281 gcaacggggc gttgcggagt tctccatcag tctggctact ggtcgggcag atatttacac
    23341 ggaaacgccg gtcaaagtgt caggctttaa gcgcgtcata gacgagcagg actggacaat
    23401 cactaaggtg acacattttc tgaataatag cggcttcacg acgtccttgg agcttgaggt
    23461 caggctttct gatgtggagt acgaaacaga agatgatgag tgatgttttt gttttatctg
    23521 tttgttttat aaggataaat taactaaaat ggcaccatca acaaaaccgg aagaggtgct
    23581 cgcgatgttt cattgtcctt tatgccagca tgccgcacat gcgcgtacaa gtcgctatat
    23641 cactgacacg acaaaagagc gttatcacca gtgccagaac gtgaattgca gcgccacgtt
    23701 catcacttat gagtcggtac agcgatacat cgtgaagccg ggagaagtcc acgccgtaag
    23761 accgcacccg ttgccgtcag ggcagcaaat tatgtggatg taattacaaa caggaagccc
    23821 ctcagtcgag gggctttttt gtcgatgtgg tcaatgtgtg gacgtgacca gaaataaatc
    23881 cttttatttc attgtattac gcgtaaaaaa taagcccgtg taagggagat tacacaggct
    23941 aaggaggtgg ttcctggtac agctagcatt ttatgggtta tgtttttcag cgaaacggat
    24001 gataacctta ataaatgcag ctgtatgtga tcggtttcta agaatttttc atccgggaaa
    24061 aataatcgaa attaatcact taccgtgggg gttacgcgtg gtttcaccgg agaaattacg
    24121 catcagcagc gcgtaattca gttcgagatc ctgcggcacc gggagccaca cagtataacc
    24181 atcgcctggt gcgaccggca ttgcttcacc tttggcgttt tccatatgct caagggtaaa
    24241 gttaatgttg ccttgcggcg tcattagctc aaggctgtcg ccaacggaga atttattttt
    24301 caccgctacc gccgcgagct cccccttgcg ctcaccggta aactcaccaa caaactgctg
    24361 gcggtcagaa accgaataac cgtattcgta gttctgataa tcgtcgtggg tatgacgacg
    24421 caggaaacct tcggtatagc cacgatgcgc cagaccttcc agcgtttcca gcaggctggt
    24481 atcgaacggt ttgcccgcag cggcgtcatc gatagcttta cggtaaacct gcgcggtgcg
    24541 tgcgcaatag tagaaagatt tggtacggcc ttcgattttc agcgaatgca cgcccatttt
    24601 ggtcaggcgt tcaacatggg cgatggcgcg cagatctttc gagttcatga tgtaagtgcc
    24661 gtgctcatct tcaaacgtgg tcatatactc gcccggacgc tgggcttctt cgatcataaa
    24721 cactttgtcg gttggcgcgc cgatacccag cgtcggctca acattttgca ccggaattgg
    24781 ctcgtacttg tgtacgatgt tgccaacgtc atcttctttc ccttcctgga cgttgtactc
    24841 ccagcggcag gcgttggtgc aggtaccctg gttcgggtcg cgcttgttga tatagccaga
    24901 gagcaggcag cgaccggagt aggccatgca cagcgcaccg tgaacgaaga tctcgatctc
    24961 catatccggc acctgattgc ggatctcttc aatctcttcc agcgacagct cgcgagagag
    25021 gatcacgcgg gtcaggccca tttgctgcca gaatttcacc gtcgcccagt tcacggcgtt
    25081 agcctgtacc gagagatgga tcggcatttc agggaagtgc tcacgcacca gcataatcag
    25141 ccctggatcg gacataatca gcgcatccgg ccccatttcc accaccggtt tcaggtcacg
    25201 gataaaggtt ttcagcttgg cgttgtgcgg tgcaatgttg accacgacat aaaacttttt
    25261 ccccagcgcg tgggcttcat tgatgccgag ctgaagattt tcgtggttga attcgttgtt
    25321 gcgcacacgc agggagtaac gcggctggcc cgcataaaca gcatctgcgc cataagcgaa
    25381 agcgtaacgc atatttttca gcgttcccgc cggggaaagg agttccggtt taaacataat
    25441 tctctcgttc tgatgacagg tcagatccgc ttcacctgat gaagcggcca ggggagtgcc
    25501 cccactttaa gggcgggcat tgtagcgctg taggggggag ctggtaaagc gccggacgcc
    25561 aacaacacgc gcccggcatc gtaattacgt attgtctttc accgtctggc ttgtgccatt
    25621 ggctttaacg gaggcaattc cggtttcacg agattgcgcg gtggcgtaca tctggctgga
    25681 gccgataatt tgatgattgg cggctttcag attgaaatag aatttgccgt tgcttgccgt
    25741 ttttttctca tagcgttctt cttgcgggct gttgctacgc accgacgcga tgcccttttc
    25801 cgcagaggct tttgaggtat acagctcgct ggtgaggata gtctcaccat tgcccgcttt
    25861 tagcacaaac cgaaattgat tatcactgct cttactgagt tcaaaccaac cagccataat
    25921 gactccttga ttgaaattgc gtatcgaaaa cagcatctaa aggtgctgtt taccgattaa
    25981 agtatgagcg attttctggt gcctgccagg gcggagagat aatgtcagtt tgctgaccat
    26041 acgtgggata atgttgccgg atctgatctg aacgccatct ccggctaaca aagtcatgtc
    26101 gctaaaacta aacgatgcgg caagcgtcgg cttcccagcg ataaccgaca ccgtagacgg
    26161 cgcggataaa agactgctct tcgtccagag cttccagctt gcgacgcagg tttttaatgt
    26221 ggctgtcgat ggtgcggtca gttactacgc ggtagtcgtc ataaagatga ttgagcaatt
    26281 gctcgcggga gaacactttt cctggttcat gagagagcgt tttcaacaga cgaaattccg
    26341 caggcgtcag gtcaagcatt ttaccgcgcc atgaagcctg aaaacgacct tcgtcgatca
    26401 tcaacgggct ttcggcatcc tgttgctgaa gttcgcgttg cggtttacaa cggcgcaaaa
    26461 tggttttgac acgcgctacc acttcccgtg ggctgtacgg cttgcagatg tagtcatcgg
    26521 cgccaatctc cagccccagc aggcggtcga tctcttcgat ttttgccgtc accatcacga
    26581 tcggaatgtc agaaaaacga cgaatttccc ggcacagcgt caggccatcg gtgccaggga
    26641 gcatcagatc taacaggatc agatccggtg gtgtctggcg cacatacggc agtacctgat
    26701 cgccgtggct gataagcgtc ggcgcatagc tcgcggcacg cagataatca atgagcaact
    26761 gtcccagctt cggttcatct tccacgatca aaatacgcgg tgtgttttcg tcgattggta
    26821 actcggtcat acttctctct gtaaatcccg ttccagcggt aactctactg taatgcttac
    26881 cccgccaaaa ggcgaatggg cagcaataat gcggccatta tgtgcttcaa caatgttcag
    26941 gcaaatcgcg agtcccagcc cggaaccgcc gctggcacgg ttgcgggaac cttcggtgcg
    27001 ataaaaacgt tcaaacaatt tttgtagctg atcgtcactg acgcccggcg cgctgtcggc
    27061 aaaggtcagg cgcaccgttt tgtcgtgctg ctcggcagag attttcaggc tgccgccgct
    27121 gtcagtgtag cgcaggctgt tttccagtaa gttattgaac aactgcatta aacgatcgcg
    27181 atcgccaaat acggtaatac tgtctggcag ggaaaattgc agtttcaggc cacggctggc
    27241 aaatcgttcg cgaaatgcgc cgcccgccac ttccagcagt gggatcaaat ctaccggtgc
    27301 tttttgatag gcgagagcgc cttcatcaga catcgacaac tgatgaagat cgtcaaccag
    27361 tttggtcagt gtaccgacct ccgcctgtaa agaagccagc gtctccggcg tgaatttacg
    27421 cacaccatcc tgaatggctt ccagttcgcc gcgcagtact gctaaaggcg tgcgcagttc
    27481 atgagaaata tcggccataa aatcgcggcg catctgctgg tttttctcca gtgtgctggc
    27541 gagttgattg aaatcctctg ccagtctgcc caactcatct tcgctggtgg gggctacgcg
    27601 agtagtgaaa tcacccgctg ccagtttgtg cgtgccatcc accagtcgtt ttaccggagc
    27661 cagcaaaccg cgcgccagtg ggaatgtcgc cagcgccgct aataaggtag ataaagcaac
    27721 aatcagccaa ctggtttgcc gctgttgtct gtcaaaattg atatcagtat tacgggtcag
    27781 tcgttcaaca ggggaggcga tcaccgcgcc aacttccgca ccattgacca taatgggccg
    27841 ccgtgtgccg tcgggtggaa ccggtgctcg cgggccaacc agcactttat tattttgatc
    27901 aaccacccaa aattgcgtgc gccagccgtg tggtggcata ccggggccgg gcttatcttc
    27961 gttgttatca tgctccagtg aacgtaggat ctgaaataca aagcgatcgt tattacgcag
    28021 gaagcgccag ttcccgtgct gggcgtactg ctcaccaagc gcatcgccga gcatttgcag
    28081 ccgttgttca ttaccatgct tgatgtaatc gataaagccg cgctcaaaac tgatacgtac
    28141 cgcccagtgc atcgtgatca ataagacaat gcaggtggcg aaaatcgcca gaaacagttt
    28201 gccggtaata ccgggtcgcc agaacttcat tgcgcgctcc tttttcgccg cgaaatagct
    28261 acattttgat gcgtatcgct cggcactctg gcaaagacaa acgccggaag ggcgatgata
    28321 aacgccatgc taagccaggt gtacataaag acggtttgtg tggtgccgct gtcgacgctg
    28381 acatgctgtg aaccaaaaag ccccagcaac agcccggcga tagtgacgcc gatactcatc
    28441 gacaattgca taatcatcga cagcagactg ttgccgctgc tcgccagatt gtccgggaga
    28501 tctttcagcg tcagggtgtt catggaggag aaacgggtcg agttgaccat cccttgtaaa
    28561 aacaggacga acggcaaaac gtagtaccag cccagcagag cagtagtcat aaacaacagg
    28621 gtgaccagcg acagacccag cgtggtcgcc accagtaccc gacgataacc aaagcgattc
    28681 actacctgta ccacaattcg cttcatcccc atgctgccga gcaccatcgg gatcatcatc
    28741 agtccggcat gaaacggtga gaagccaagg ccaatttgta ggaaaaccgg cgtcataaag
    28801 ggcaacatgc cgctaccgat acgtccggca aaactccccg ccagacccag cgaaaaggta
    28861 cgagtacgga acagtttcag actgaacagg gcgcagttgt tatttctggc gtgcagcaga
    28921 taaagcacca gtgccaccac gccaactgcg accaggccag caatcgccag cggcgataaa
    28981 cctgtacctt tactgccgtc cagcgccagg gttaataccg ccatgccaac cgccagcaat
    29041 aaaaagccgg agagatcaaa gcaccgcgtc tgcatggtgt agttcggcat taacatcagt
    29101 gtggtgatcg cgccaataat ccccaccgga atgttgatca aaaagatcca gtgccacgat
    29161 gcgtactcca ccagcagacc gccgagcgcc gggccaagca gcggcccgat ctgaccgggc
    29221 agcgtgacaa aggtcatcgc cgccatatat tgctcgcgcg gtacgatttt catcaccgtc
    29281 aacctgccga ccggcaccat catcgcgccg ccaacgccct gtaacgcgcg tgccagcaac
    29341 agttcgttca gcgtgccgga aagcgcgcaa aacagtgaac cgagagtaaa cagcacgatg
    29401 gcggtaaaga aaatattgcg cacgccgact ttgtccgcca gccagccgct ggcgggcagc
    29461 atcaccgcca cggtcagtac ataagagaca atgaccatgt gcatatgcaa cggactttcc
    29521 ccgaggcttt gcgccattga gggaagcgcg gtgttaacga tggtggtgtc cagcgactgc
    29581 ataaagaagc cgaaagccac aatccacaat tgccagcggg tgctgtcggg aagatctgtc
    29641 atttactcgg ttaccgcttg tttaggttta cgcgaaaaac gcagtcgcag acggtcgaaa
    29701 aagagataca ccaccggcgt ggtatacagc gtcagaagct ggctcatgac cagaccgccg
    29761 acaatggtga tccccagggg ttgccgcagc tccgaaccgt caccgcccga caataccagc
    29821 ggcagcgcgc caaacagcgc cgccagggta gtcatcataa tcgggcgaaa acgcagcaga
    29881 caggcctgga aaatagcttc ctgcggcgtg aggttaccgt gccgttgggc ttcaagcgca
    29941 aaatcgacca tcataatggc gtttttcttc acgatgccga ttaatagcat gatccctatc
    30001 agggcgatta ggctgaacgg ggcattgaac agctccagcg ccagcagcgc tccaacgccc
    30061 gccgagggca gggtggagag aatcgtcagc ggatgtacgt aactctcgta aaggataccc
    30121 agcacgatat acaccgtggc gatggcggcg ataatcagga tcacctgcga gttcatcgtc
    30181 tcctggaaca cctgcgccgt gccggcaaaa ctaccgcgca ccgtcgaagg cacaccaagc
    30241 tgggtcattg cgcgatcgat cgccgcactg gcgtccgaga gcgatttccc ggtcggcagg
    30301 ttgaacgaga tggtcgaggc tgccgataat ccctgatgat tcaccgacag cggagcattc
    30361 gccggttgcc atttagcgaa atacgacagc gggatcgctt ttccttcgtt attgataacg
    30421 aacatttttt ccagcgcact gatgtcctgg gtatagcgcg gatccacttc catcaccact
    30481 ttatactggt tcatcggctg gtaaatggtc gagatttgtc gctgaccgaa ggcgttattt
    30541 aacaggctgt tggcggcctg cacgtcgatt cccagccgtg ccatggtgtc gcggtcgtaa
    30601 accagattca tctccgcgcc gttatcctgc tggtcggagt tcacgtccgc cagttccggc
    30661 aatgtcgcca gttttttgcg aattttcggc tcccattcgc gcagtgccgc caggtcgtcg
    30721 gacagcaacg tgtactgata gctggcgttc gactgacgtc cgccaacgcg aatatcctgt
    30781 accgccatca ggaacagatt cgcccccggt tctttcgcca gcttcacgcg cagacggtcg
    30841 ataatttgct gcgccgtttc gctgcgttcg ccgcgtggct tcagcgtgat aaacatcatc
    30901 ccgctgttca cccgcgaacc gccggtaaat ccggtgacat tatccactgc cggatcgtca
    30961 cggataattt tcataaaatc ctgcaactta ccgcgcatcg cctgaaacga aatactctga
    31021 tccgcctgta tcccgcccat caacacgccg gtgtcctgct ccgggaagaa ggttttcggg
    31081 atcgagatat acagccagat attcaaagca atggtgccaa gcagcaccac gcccaccaga
    31141 cgggtatgat taagcaccca tttcagcgac ttaccatagc cttgttgcag ggctaccaac
    31201 atgcgaccaa aaccacgtag tcgcttttgc tcgcgcggct tactggcttt tagcatccag
    31261 ccgcacatca ttggcgttag cgtcagagaa accagcagcg aaataccaat ggcgacagaa
    31321 agcgtcacgg caaactcgcg taacaggcga cccggcaatc cgcccatcaa cagcagcggc
    31381 agaaacaccg ccaccagtga cagactcatc gacagcaccg taaaaccgac ttcgcgagtg
    31441 ccttgcagcg cggcctgtag cggtttcatc cccgcttcca ggtgacgggc aatgttttcc
    31501 agcaccacga tggcgtcatc caccacgaaa ccagtagcga tggtgagcgc cattaacgaa
    31561 aggttattga gactgaatcc gcacaggtac atcgccgcaa acgtaccaat cagcgaaacc
    31621 ggcaccgcaa cggcgggaat aatagtggcg cgacccgagc gcaggaataa aaagaccacc
    31681 agaatcacca gcgccaccga gataatcagc gtttgctcga cttcctccag cgaggcgcga
    31741 atggtggggg agcgatcctg ggcaatttgc agatcaatcg ccgccggaat ggtctcctgc
    31801 aactccggta attttgcccg gatgctgtca accgtctgga taatattggc ttccggcagc
    31861 ttgcggatca tcagcaaaat agccggtttg gcgttggtca tcccggcgtt gcgcacatcc
    31921 tgcaccgagt cggtcaccgt cgccacatcg cccagacgaa gcgcgccgcc gttgttgtaa
    31981 tgaataatca acggctgata ttcggcggcg gttttcagtt catcgttagt ctggatctgc
    32041 cagcgatggg tatcatcttc cagcgccccc tgcggcttac gcacgttggc attgctgatg
    32101 gcgctgcgca catcgtccag cgacacgccc tgattaaaca gcgcctgcgg attcagcccg
    32161 atgcgtacgg cgggcagtga gctgccgcca acatcgacat caccgacacc gtcgatttgc
    32221 gaaatcgtcg gagccagctg cgtcgaggcg aaatcgtaca gttcaccctg cgaataagta
    32281 tcggatgtca gcgtgaggat cataattggc gcatccgacg ggttcgcttt gcgataggtc
    32341 ggacggcttg gcatcccgct gggcagcaaa ctttgcgcgg cgttgatcgc cgcttgcaca
    32401 tcgcgcgctg cgccgttgat atcgcggtca aaatcaaact gcaaaataat gcgcgtgctg
    32461 ccgagcgaac tgctggaggt catttcactg accccggcaa tgcgtccaag cgaacgttcc
    32521 agcggcgtgg caacggaaga cgccattgtt tctggtgacg caccgggcag cgaggcgctg
    32581 accataatca ccggaaaatc gacctgcggc agcggggcga ctggcagcat acggaaaccc
    32641 agtatgccgc acagggtaat ggcgaccgac agtaaaatcg tcgccaccgg gcggtaaatg
    32701 aagagggcaa aaaacttcac ttacgcctcc tcttcatgac gagcaaagcg gcttttggtc
    32761 cacaatgcca ggcggtcgaa tagcagataa atcaccggcg tggtgaacag cgtcagcacc
    32821 tggctgacaa tcagaccgcc gaccatgccg atacctaacg gacgacgcag ttccgcgccg
    32881 accccggtac tcaacatcag cggcagcgcg ccaagcagag ccgccagggt ggtcatcagg
    32941 atcggacgaa aacgcaacag acaagcctgg tagattgcat cgcgcggcga catgccttgc
    33001 tcgcgctcag cagccagcgc gaagtcgatc atcatgatgg cgttcttctt cacgataccg
    33061 atcagcaaaa taatgccgat aatcgcaatc acatccagct cgctaccggc aatcagtaac
    33121 gccagcagtg cgccgacccc tgcggtgggt agcgtcgaga gaatggtgat cgggtgaata
    33181 aagctctcgt acagaatgcc gagcacgata tacatcgcca ccaccgccgc gacaatcagc
    33241 cagacagtgc tgcccaacgc cgactggaag gcgagggtgc tgccctggaa ctgcgtggtg
    33301 atatccaccg gcagattcag cgtcttttcg gtgtccataa tcgcctgcac cgcatcgccc
    33361 agcgaatagt tatccggcac gttaaaggag atggtcgtta ccgggaactg atccagatgg
    33421 ttgatggaaa gcggcgcaaa acgctgctca attttggcaa ttgagcttag cggtaccacg
    33481 ccgccgtcgc tgctggtcag gcgaatagta tccagcgccg caagcccagg agtattttcg
    33541 gtgttgtgct ccagcaccac gcgatactgg ttggcctgag tataaatagt ggaaatcagt
    33601 cgctgaccaa acgcgttgta aagggcgtta tcgacgtccg ccatgctgat ccccagacgg
    33661 ctggcgctgt cgcgatcaac attgacatac gccaccagcc ctttgtcctg ccagtcgctg
    33721 gagacatcag aaagctgtgg cagttgctgg agtttttcca tcaactgtgg cacccaggta
    33781 ctgagcgcat ccagtgacgt ggcctgcaag gtaaactggt actgggtgcg gctgacctga
    33841 gtatcgatgg tcaggtcctg cgttggttgc aggaagagat cgacgcccgg cactttatcc
    33901 accgccgttt gcaggcgggc gatgactttt tgcacccgat catcacgttc atccaacggt
    33961 ttgaggttga tctgcaaacg cgcactgttc agcgacgggt tagtgccatc aacgccaaca
    34021 aatgaggtca gactttgcac cgccggatct tgcaaaatca catccgcgac ctggcgttgc
    34081 cgctgggcca tattggtaaa ggagctggat tgcggtgcct gcaaagtgcc ctgaataatg
    34141 ccgttgtcct gtaccgggaa gaaacctttc ggaatgaaca cccacaacag cacgctaagc
    34201 agcagcgtgc tgagcgccac gcttaaggtc agccacggat gattcagcac tttcgcaagt
    34261 ccctgaccat aggcggcgat tatcctgtcg aacatttttt ccgaggcacg ggagaagcgg
    34321 ttctgtttac gcagcgactc ctggctgagc atccgcgcac acatcatcgg tgtcagggtc
    34381 agcgacacca cggctgagat caaaatcgcg actgccagag taatagcaaa ttcgcggaac
    34441 agtcgcccga cgatatcgcc cataaacagt agcgggatca gcactgcaat cagcgagaag
    34501 gtcagcgaga taatggtaaa accgatttca cctgcgcctt tgagcgccgc cgccagcggt
    34561 ttttcgcctt tttcgatata gcgggaaatg ttttcgatca ccacgatggc gtcatcgacc
    34621 acaaatccgg tggcgatagt taacgccatc agcgtcaggt tattgattga aaaatcgaga
    34681 aacaccatga ccgcgaaggt gccgattaac gacagcggca ctgcgacgcc gggaatgatg
    34741 gtcgccggaa tattacgcaa aaacaggtag ataatcatga ctaccagcgc gatagccatc
    34801 atcaattcaa actgagtatc atccacggat gcgcgaatat tggtggtacg gtcagaaagt
    34861 accgtcacct tcaccgattt cggcagactc tcggtgagct gtggcagcat ctgccgaatg
    34921 ctgtcggcgg tggagataat gttagcaccg ggctggcgct gaacattcat cacaatggcc
    34981 tgttctttgt tagcccatgc gccaagccag ctgttttctg caccttgctc tacggttgcg
    35041 acatcgccca gacgaatcgg cgcgccgttc tggtaggcga tgattagctg gcgatactct
    35101 tcggcggatt gcatctggtc gttagcggaa agcgtaaccg cacgagaagg gccgtcgagg
    35161 ctaccttttg ccgagttgac gttagcgccg gtaatggcgg tgcgcacggt ttcgctggtc
    35221 aggccgaggg cggcaatcgc ctgagcgtta agtttgacgc gaacagccgg acgctgaccg
    35281 ccggaaagtg tcaccaggcc gacgccggaa atctgcgaga ttttctgcgc gacgcgggtt
    35341 tccaccatat cttccacttg cgtcatcggc atggcggttg aggtgacagc gagcgtcatg
    35401 atcggcggat ctgccgggtt cactttgctg taaaccggcg ggttaggcag atcgctcggc
    35461 aacaagttgg tcgcagcgtt aatcgcggcc tgcacttcct gctcggcgac atcgagcggt
    35521 aatgttagct ggaactgcaa agtgataact gacgcaccgc cggaactttg cgacgacatc
    35581 tgtttcaggc cagacatctg cccaaactgg cgttcaagcg gcgcggtaac ggcagaggtc
    35641 atgacatccg ggctcgcacc tgggtagagc gtgactacct gaatggtcgg atagtccact
    35701 tccggcagcg ccgaaacggg cagggctcga taaccgataa tcccggcgag taagatcgcc
    35761 accatcagca gcgtggtggc gacaggacgc ataataaaca ggcgcgacgg gccgcctgtg
    35821 ctgctcgggg gtaacacctg catcaggagc gtgctccttt tttcgcgtat tcgcggctgg
    35881 tggctttctc ttccggagtg gtggcgctct gggcttccac tacttccact ttcgcccctt
    35941 cggtcaggcg atcaatgccg tctgtcacta cgcggtcgcc cgcagaaata cctgcgcgga
    36001 tcaccacttt ctgactgtcc tgaatgcccg gcgtcaccag atgtttgctg accttgtttt
    36061 cgctattcag cacccagaca aaatggcctt cattgcccat ttgcagggcg gctgttggga
    36121 tcaccacagc gttttgctct gtgtcgacta acatgcgcgc gttaacaaac tgattgggaa
    36181 acagcgcatc atcctgatta ttgaagcgtg ctttcacttt aatcgtaccg gtagtggcat
    36241 cgatttggtt atcgagactt aacagcgtgc cttcacttaa tttctttgaa ttggtgcgat
    36301 cccaggcttc aacaaccagc ggttttccgg ctttttgcgc ctgcactacg gtagcgatat
    36361 cgctttccgg cagggtaaag agcaaatcga taggatgcgt ctgggtgatc accacaatcc
    36421 cggtggtatc accactggag atttggttac caacatcaac ctgcttgaga ccaacgcgac
    36481 catcgactgg tgcggtgatg cggctccagt cgagttgcag ctgcgcgctg gcgacgcttg
    36541 cttcatcagc cttaatggtg ccttcggttt cactgaccag cgcctgttgg gcatccagct
    36601 cctgacgaga tacgagattg gtttttacca gttgttgata acgcgccagg tcacggcggg
    36661 cgttggcaag cgtggcttta tcttttgcca gttggccctg ggcttgtgct aatgcaactt
    36721 tgaactggct ggggtcaatt tctgccagta aatcgcctgc tttgacctgc tggccttcct
    36781 ggaaatgcaa cgccatcagt tgaccgtcca cgcggctgcg caccgtaaca gtattagcgg
    36841 cggtaatggt gccaagcccg gtgaggtaac gcggaactgc ctgttctacg gcggtcgccg
    36901 cctgaaccgg ggctaatggg ccggaacgca taccacggcg accacccgct ggcgattgct
    36961 gcgcttgttt cgtcgcccct ggcgctgcac tccgggagtc attgcggcct tgccagaacc
    37021 agaatgcggc gatggcggcg ataaccacca cgattaccat tacccaacgg gatttataac
    37081 tgcttttcat cgttaagagt ttctcttcct gaaacgattc gcggaatgat actagtgtag
    37141 ccagccaatc agggagaaaa atggaggaat tatgaaacac tatcgggaat cgtctgaatg
    37201 gcgggcacat ttgcgagcac ccatccagta ataacacagg aaactatttt atctacgcgt
    37261 tagcgataga ctgcattcag ggcaaaagga ggtaggccga cgatttcagc gggacgctga
    37321 aacgggcaag cccctcccga ggaaggggcc ttaaataagg aaagggtcat gatgaagata
    37381 ctcatcatcg tggtgctctt agtcgttagc tacccagctt actaagacta ccagggcggg
    37441 ggaaaccccg ctctaccctc actcctgaaa gtatgccttc acggtccgat tgtcaatccg
    37501 caggctttgc agtctgcgat cctgccagca aatattcttt gcgagacgtc acgcaataat
    37561 cacatgtgaa actattttat ccgcaagtca gcgatagact gcattcaggg cgaaaggagg
    37621 taagccgatg atttcagcgg gacgctgaaa cgggaaagcc cctcccgagg aaggggcctt
    37681 aaataaggaa agggtcatga tgaagtggat catcatcgtg gtgctcttag tcataagctt
    37741 cccggcttac taagactacc agggcggggg aaaccccgct ctaccctcac tcctgaaagt
    37801 atgccttcac aattagattg tcaatccgca ggctttgtag tctgcgatcc tgccagcaaa
    37861 tattctttgc gagacgttac gcaataatca catgtgaaac tattttatct gcaagttagc
    37921 gatagactgc ttgcatggcg aaaggaggta ggccgccgat ttcagcggga cgctgaaacg
    37981 ggaaagcccc tcccgaggaa ggggccttaa ataaggaaag ggtcatgatg aagctactca
    38041 tcatcgtggt gctcttagtt attagctggc cagcttacta agactaccag ggcgggggaa
    38101 accccgctct accctcactc ctgaaattat gccttcacga taagattgtc aatccgcagg
    38161 ctttgtagtc tgcgatcctg ccagcaaata ttctttgcga gtcgttacgc aataatcaca
    38221 gaggaaacta ttttgttcac gcgttagcga tagactgcat tcagggcgaa aggaggtaag
    38281 ccgacgattt cagcgggacg ctgaaacggg aaaccctctc ccggagaaga gggcttttaa
    38341 taaggaaagg gttatgatga agcacgtcat catactggtg atactcttag tgattagctt
    38401 ccaggcttac taagaacacc agggggaggg ggaaacctct ccctaaccct cactcctgaa
    38461 attatgtcac tccgccctta gtgtcaatcg acggctctcc gttcttgcga ggcgaatagc
    38521 aaaattttct gcgccgccgg gcctaattct gaaagtttct tcgtttgctc ttatttcatt
    38581 gttgcagtcg gtatgatacc gggcctcaat aattctctaa gaaaggattt cttatgagcg
    38641 aacaaatcac gtttgccacc agcgattttg ccagtaaccc ggaaccacgt tgcccctgca
    38701 ttttgctgtt ggatgtttct ggttctatga acggtagacc tattaatgaa cttaacgccg
    38761 gattggttac ctttcgcgat gagctgcttg ccgatccgct ggccttaaaa agagtggaac
    38821 tcggcattgt gacgttcggc ccggtgcatg tggaacaacc atttaccagc gccgctaatt
    38881 ttttcccgcc catcctgttt gcccagggcg atacaccaat gggggccgcc attaccaaag
    38941 ccctggatat ggttgaggag cgcaaacgtg agtatcgcgc caatggtatt tcctattatc
    39001 gtccgtggat tttcctgatt accgatggcg caccaaccga tgagtggcag gctgctgcca
    39061 acaaggtgtt tcagggggaa gaagataaga agtttgcctt cttttccatc ggtgttcagg
    39121 gggcggatat gaagacgctg gcccaaatca gcgttcgtca gcctttgcct cttcaaggat
    39181 tacaattccg cgaactattt agctggttat ccagttcgct gcgttcggtt tcccgctcca
    39241 cgccgggaac ggaagttgtg ctggaagcgc caaaaggctg gacgtcagtg tgagctggcg
    39301 tctggtctat gcatcaaccg ttggaacgtc gcacatcagc gcggacttgc cttgccagga
    39361 tgcctgccag atgcaggttg cctggttaaa tgaccagcag ccattattgt cggtatttgt
    39421 cgctgatggc gcaggtagcg cctcgcaggg aggcgaaggt gcgatgctcg ccgtcaatga
    39481 agcgatggct tatatgtcgc aaaaagtgca gggcggggaa ttggggctta atgatgttct
    39541 cgcaacggac attgtgctga ccattcgtca gcgacttttt gccgaggcgg aagccaaaga
    39601 attagcggtt cgcgattttg cctgcacgtt tttgggattg atttcatcag ctaatggcac
    39661 cttgattatg cagattggcg atggcggcgt ggtggtagat ttggggcacg gtttgcaact
    39721 gccgctcacg ccgatggtgg gtgaatatac caatatgacg cacttcatta ccgatgaaga
    39781 tgccgtttcc agactggaaa ctttcaccag cactgagcgt gcgcacaaag ttgcagcatt
    39841 tacggatggt atccagcggc tggcgttaaa tatgctggat aattctccgc atgtgccttt
    39901 ctttaccccg tttttcaatg ggctggcgtc agcaacgcag gaacaactcg atttattacc
    39961 tgaattgtta aagcagtttt tgtccggccc ggcggtaaac gagcgtaccg atgatgataa
    40021 aacgttggca ctggcgctgt ggttaccatg aaacccactt tatatactgc tactggtgag
    40081 tgcgttacgc caggctgtga actgggcaaa ggcggtgaag gcgcggttta tgatatcgag
    40141 gagtttgtcg atagcgtcgc caagatttat cacacgccgc cacccgcctt aaaacaggac
    40201 aaacttgcct ttatggctgc gacagctgac gcgcagttgt tgaattatgt cgcctggccg
    40261 caggcaacgc ttcacggtgg acgaggcgga aaagtaatcg gttttatgat gccaaaagtt
    40321 tctggtaaag aaccgattca tatgatctat agcccggcac atcgtcgtca acgttaccct
    40381 cattgtgcgt gggattttct actctatgtt gcgcgcaata ttgcttcatc ttttgctacg
    40441 attcacgagc acgggcacgt cgtgggtgac gtaaaccaga acagctttat ggtaggtcgc
    40501 gacagcaaag tggtgttgat tgatagcgac tcctttcaga ttaacgccaa tggcacgctg
    40561 catttatgcg aagtcggcgt gtcgcatttt acgccgccag agcaacaaac cttgccatca
    40621 tttgtcggct ttgaacgcac cgcgaatcac gataattttg gccttgcgtt gctgattttt
    40681 cacgtcttgt ttggtgggcg gcatccttat tccggtgtac cgcttgtctc tgatgcgggt
    40741 aatgcactgg agacggatat tgcccatttc cgttatgcct acgcgtcaga taatcagcga
    40801 cgtggtttaa aaccgccgcc acgatctatt ccgctgtcga tgttaccggg catgttgaag
    40861 ccatgtttca gcaggcattc acggaaagtg gcgtggcaac cgggcgtccg acggcaaaag
    40921 cgtgggtagc ggcacttgat ttactacgcc aacagttaaa gaaatgtacc gtttcggcaa
    40981 tgcatgttta tcccggtcat ttggctgact gcccgtggtg tgctctggat aatcaaggcg
    41041 ttatctattt tattgatctc ggcgaagagg tcattaccac cagcggtgat tttgtgctgg
    41101 cgaaagtctg ggcgatggtg atggcgtcag tagcaccgcc agcattgcaa ttaccattac
    41161 ccgatcattt ccaaccgact ggcaggccgc ttcctttagg cctgttaagg cgtgaataca
    41221 tcattctgat tgagatcgca ctgtcagcgt tatcgctgct gctttgcggc cttcagacag
    41281 aaccacgtta tattattttg gttcctgtgc tggcggctat ctggattatt ggcagcctga
    41341 caagcaaagc gtacaaagca gaaatccagc aacgccgtga ggcgtttaat cgcgcgaaaa
    41401 tggactatga ccatttagtc agccagatcc agcagttggg cgggctggaa ggttttatcg
    41461 ccaaacggac gatgctcgaa aaaatgaagg acgaaattct tgggttaccg gaagaagaaa
    41521 agcgcgatct ggcagcactt caggacaccg caagggaacg gcagaagcag aagtttctgg
    41581 agggattttt tattgatgtt gcctctattc ccggcgttgg ccctgcgcgt aaagcggcgt
    41641 tacggtcctt tggtattgaa acagcagcgg atgttacccg acggagcgtt aagcaagtga
    41701 aaggttttgg tgatcatctg acccaggcgg ttatcgactg gaaagcgagc tgtgaacgcc
    41761 gttttgtgtt caggccgaac gaagcggtaa cgcctgcaga cagacaagcg gtaatggcaa
    41821 aaatggccgc caaacgacat cggctggaat cggcgctgaa tgtcggtgcg acagagttgc
    41881 agcgattccg ccttcatgct ccagcacgga ccatgccgtt gatggaaccg ctacgtcagg
    41941 cggcagaaaa actggctcag gcgcaggctg atttaagccg ttgctgagcc agttttcaat
    42001 taacgaaaca ccacttccgc ccagcgtgcc agcccggcgg tgacggagcc aaagtcatcg
    42061 ccacctgcaa tcggaatgcc cggcaactgt tctgtcagcg cttttttaat cagcggtgaa
    42121 cgggcgctac cgccggtcag atagataaca tccggttttt cctgggcgtt atccagcgcc
    42181 agttgcacct gttccagaat ccgcgccagt ggctggctga gggcgctttc cagcccttgc
    42241 tggctaatca gcgtagccag ttcatcgctg ataaacggca gtgaggcgcg ggtttccgct
    42301 acgcttgaaa gcgcaatttt gctctcttct gcgctacgta ccaggcgata gcttaaacgc
    42361 tgacgccaga ctttctgtaa cagggccact ttttccggtt cgcgggcatc gcgtaccagg
    42421 tcgttaagca gacgaccgtt ggcactactg tagaaatcac tttgcgcagg aacgtcgttg
    42481 atggcaaccg cattccacca cggcagaatc ggcagggcga tgcctttttc ggtttcgcca
    42541 cccatgccca gtaacggcat caggttttta aacgccagtg cgatatccag atcgttaccg
    42601 ccaatacggc aaccactgtg acccagcagg ctggcttcac gatcgagacg cgaacgccac
    42661 tgcggcccca tcagcagcaa tgaacagtca gtcgtaccac cgccgatatc caccaccagc
    42721 acccgttttt cttcctgcaa ggtggcttcg taatccagcc cagccgcgac cggctcgtac
    42781 tggaatacca catccttaaa cccggcacgc ttcgccgcgc gttccagaat cccttgcgcc
    42841 tgggcgtttg cttcatcgcc gcccagcccc tggaagttga tcgggcgacc aatcaccgcc
    42901 tgagtaattg cctccggcag ctgcgcctgc gcctgctggc gaatgtgcaa catcattgcg
    42961 cagaccagat cctcaaacag cgctacctgc tgcggtttta agccgctggc accgaggaat
    43021 gattttggtg atttcacaaa ccacacctct tctggatcat caatgtactg tgccagtgag
    43081 gaaagaccga actgcacgct tttcgccgta acatcgatat cttcttcgcg gttataacga
    43141 atcgcccgac gcaacagcgc ctgcgtttca tcgtcgtctg ccggaacatc atgatggcgg
    43201 tacagccatt cgcttaccgc ttcacgcgtt ggcgcgcaaa gcattgaagg cagcagcgtg
    43261 ctgtcgtttt ccattttcag taattgcggc ttaccgtcgc gcatgaccgc cactgaacag
    43321 tttgctgtac cgtaatcaaa accaataaac acgaaataat ccccatgccg gtgaagaagg
    43381 ggcgtgactt tagcgaaatg ttgccgtcgc gacaaccgga atatgaaagc aaagcgtagc
    43441 gtctgaataa cgtttatgct gaaagcggat taacaaggag atgcgatgta taccctgaac
    43501 tggcagccgc cgtatgactg gtcgtggatg ttgggatttc tcgccgcccg tgcggtgaac
    43561 ggtgtggaaa cggtcgctga cgattattat gcccgtagtc tggcggtggg cgaatgtcgc
    43621 ggcgtggtga ctgctattcc ggatatagcc agccatactc tgcacataaa tttaagtgca
    43681 ggtttagaac ccgttgccgc agagtgtctg gcgaaaatga gccgcctgtt tgatctgcaa
    43741 tgtaacccgc agattgttaa cggtgcgttg ggcaagttag gcgcggcacg gcccggattg
    43801 cgtttacccg gttgtgttga tgcttttgag cagggcgtgc gggcgatttt aggccagctg
    43861 gtgagcgtgg cgatggcggc aaaattgacc gccagagtgg cacagcttta tggcgaaagg
    43921 ctggatgatt ttccggatta tgtctgcttc cccacgcctc agcggctggc agcagcagat
    43981 ccgcaggcat taaaagcgtt aggtatgccg ttgaaacggg cagaggcgct gattcatctg
    44041 gcaaatgcgg cgctggaggg caccttacca atgacaatac cgggcgatgt ggagcaggcg
    44101 atgaaaacgc tgcaaacttt tccgggtatc gggcgctgga cggcgaatta tttcgccttg
    44161 cgcggctggc aggcgaaaga tgttttcctg ccggatgatt atctgattaa acagcgattt
    44221 ccgggaatga caccggcaca aatccgccgt tatgccgaac gctggaagcc cttgcgttct
    44281 tatgcgctgt tgcatatctg gtatacggaa ggctggcaac cagacgaagc atgacgttgg
    44341 tcgccggatg cggtgcgagc gccttatccg gcctggggaa tgagcgattt gtaggcatga
    44401 taagacgcgt tagcgtcgta tcaggcgatg gagaagcacg cctcagttaa tcgcgaaata
    44461 actgctattc accagcaaat ccagcggttg ggcatcggca atcacatcgc cataaatcag
    44521 atcgacgccg atgccagaaa gcgtatccat caccaggggt aaaactactg gcccggcgat
    44581 ggtcttcatc ccgagccgct gagcgtgccc ctgaatgatc gtaatcagca tctcatccat
    44641 caaatttccc tgcacattgg cgcataactc accatcaagt agcaggtaat ctgccatatt
    44701 cgctttcagc gagttgaaga tttgcagatc gcggcccacc tgactgaata cgatccgaca
    44761 tcccgccagc cgcagttttt gcacgctttc tgcgtgatct aaaatcgcgt ctgccggaat
    44821 aatcagatgt aataaccgtg ggggtagagg gctattttcc agctgctcaa gcagttcatt
    44881 caccagcgtg gcgctactca aaccggcaac ggaaaggggg agggcgatgc ttaagccttt
    44941 actggcaatc gcttttgcgg cctgctggaa aaaatcgtgg aatacccgtc ggtcaagagc
    45001 atggctaagt gccggatcgc tgaagctacg acgaaatgtt tgttcatcaa taatctcgcc
    45061 ttcgcaactc cagagcttaa gtgaaatcag ccacaaatta cgcgcttccg ggatccgcgg
    45121 cgaagcgaca ccgtgggcga tcatcatcaa ctgattctct ttaatcatcc gccactgttc
    45181 atcaagcgac atcaccgccc gttcgctatg tgcggcagct tgctgcggtt cgtaaaccgt
    45241 cacccggccc cgtccaccat ttttggaggc ataacaggcg atatcagcct gcgacatcac
    45301 ttcagccgcc tgatgattgt tgtcatcaat caaggtaatc ccggcactgg cacctacccg
    45361 atgtacacgt ccttcccaga taaagtgata gtcattcacg gcgctgataa tgcgtgtagc
    45421 gataaaacgc gcactttcga cattacaatc tggcagcagc agaccaaatt catcgccacc
    45481 gagtcgcgcc agcacgtcgc tggagcgcag catactcagc atcaatgacg ccagttcgcg
    45541 cagtaaagcg tcgccagcgg cgtgcccggc gctgtcattc accgctttaa agcgatcaag
    45601 atcgataaac accagtgcat gtcgctgatg tgtactgttt accgtttgca gcaggatgcg
    45661 tagttgcttc tcaaaactgg cgcgattggc gagatgcgtc agtgcatcat gggaggcgct
    45721 gtagctcagc tggcgcagca ttttacgcga ttcggtgacg tcctgaatca ccagaaccga
    45781 gcctatattg ctgccgtcca gagtacttaa cggcgtaata ctgtaatgca cgtcgtagct
    45841 gccgccgctc cgacagtgca acaccacatc ttgttcaatc gcggaacgtg aggtgtcggc
    45901 actgtaaatg ttctccatta atggtccgtt gtcgccaaaa gtaatatgca acaccgtcag
    45961 gagcggaaca cctaacgctt cttcctgcgt ccagccgctc atcttctccg ccaccggatt
    46021 cataaaggta attttcattg ccatatcaat acagaccacg gcttcgccga tggaatccag
    46081 cgtaatgtgc aggcgctctt tttcctgaaa caatgcctcg ttaagctgtt tcacttcggt
    46141 catatccata ttaatgccga gcaggcgttc gacttcgcct tctttattca gtacccggtt
    46201 ggcgagggcg cggatatggc ggatgccatc tttcacggca atgcgaaatt ccagcttaaa
    46261 gggcgagcgt gattgcaacg aatcacgaat cactttttcg gcatgctggc gatcttccgg
    46321 cagtacgcac tcgtaccaca cctgccagtt cggtttgata tgcggaggaa tttcatacag
    46381 ctcgaacatc cgcttatccc agctaaaaat attcggcttt agctcccact cccagatgcc
    46441 aatcccgccc gcttcgttag ccagcgtgat gcgctccatc agttgctggt tcacctgttc
    46501 ggtgcgtttt agctcgttaa tgtcttcaat ctgagcgata aaatagagcg gcgtaccatc
    46561 ggtgtggcgc accagtgaga cggcaagcaa cgcccagaca acatcgccat tgcggttgta
    46621 gtagcgtttt tccattgaat aggtgtttat ttcaccgctg atcagctttt caacctgttg
    46681 cagatcttta ttgagatcct ccggccaggt cagttgctga aaggtgagtc cgcgcagctc
    46741 ttcctgactg taaccgagaa actggcagag cgctttgttg gattgcagcc attgtccctc
    46801 ggtgcccact aatgccatgc cgatggcgga atattccatc gcgttccgaa agcgggtttc
    46861 gctttcggaa atgtgtttgc gttccgcacg aaaggcatac atcaccatgg tcatgatgtt
    46921 ggcgggcagc aggatcagca aaaacggtag ccacggcata tggctcatca ggtatgtacg
    46981 cggcgtagca agcagggagg gatccgcggc catcatcagt gacaccatca tcaccgtggt
    47041 aaggaagatc aaaaaggctt ccattcgtgg caggcgcacg gcgctccaca tcaacagcac
    47101 aataatgaaa gtaaaaggcc acggcagata cagcatcgaa agccagctta acgtcagtgt
    47161 gatggctaac gtgagcagcg actcaaaaag caaccgtggg ttgcgatggc gcagcagata
    47221 gtgtggttta aataacaatc ccagcggcac cagtgccagt gcgccgatgg attctgacag
    47281 tacccatatc aaaaatgccc tgagaggatc gtctccgggc gtcagcagga taaccagaac
    47341 accccctaac agaggtggaa caatggcgct gccgagtgcc agacgcagcc agtcagccag
    47401 attttgcaat gggttatacc acggcagcaa tttacgcagt agcactgccc cgaccacggc
    47461 ttcaacaata ttgatggtcg tccaggtcat gttcagcgag ctggtggaaa aaagcaggat
    47521 ggatgcggcg atatttccca gcgagcagct cagcgcaatc cccggccaca tgcgcccggc
    47581 atggcgataa aacgccacca tcatgatgga cgtcgggaac cacagtgggg cgagttgggt
    47641 gccaaactgc gaaagctcaa gcgagaaaag ggtaaagata aacgagacta aacctaaact
    47701 gaccaggtga agcagcgggt ggggcagggc aattaataca tgctgtgatt gtttgctcat
    47761 taccgcttta tccgcaagac caggtcgcca gtattcgttt ctgtgtttat gctagtacaa
    47821 aagttttaca ttttatatgc gaattgctca taaagtgacg taaaggcgga taatttgcgc
    47881 aactgcgtat aacaattttt accttgtata aaactgatca acgtaatttg ccaccgggaa
    47941 ttcttcgtca acaattaact ctgagcgcgc ggcaacgcta ttcgactggt atcaggcgga
    48001 tgaaatccct ataattgccg cgtttggcgc ttcgtcgccc ccttcctaac atccaggtta
    48061 atcaggtcgc taaatatatg actgatcagt ctcatcagtg cgtcattatc ggtatcgctg
    48121 gcgcatcggc ttccggcaag agtcttattg ccagtaccct ttatcgtgaa ttacgtgagc
    48181 aagtcggtga tgaacacatc ggcgtaattc ccgaagactg ctattacaaa gatcaaagcc
    48241 atctgtcgat ggaagaacgc gttaagacca actacgacca tcccagcgct atggatcaca
    48301 gcctgttgct tgagcattta caagcgttga aacgcggctc ggccattgac ctgccggttt
    48361 acagctatgt tgaacatacg cgtatgaaag aaacggtgac ggttgagccg aagaaagtca
    48421 tcattctcga aggcattttg ctgctgacgg atgcgcgttt gcgtgatgaa cttaacttct
    48481 ccattttcgt tgataccccg ctggatatct gcctgatgcg ccgcatcaag cgtgacgtta
    48541 acgagcgtgg gcgttcaatg gattcagtga tggcgcaata tcaaaaaacc gtgcgtccga
    48601 tgttcctgca attcattgag ccttctaaac aatatgcgga cattatcgtg ccgcgcggcg
    48661 ggaaaaaccg catcgccatc gatatattga aagcgaaaat aagtcagttc tttgaataag
    48721 cttgataaat tgtgtaccgt tcagtgataa cctggtatgc ccttgacgta attggcgtta
    48781 agggagtgat gcgcgaaagg agaaaatgcc atgcgtctgt gtgaccgaga tattgaagcc
    48841 tggcttgatg aaggccgttt gtcgatcaac ccacgtccgc cagtggagcg tattaacggc
    48901 gcgacggtgg atgtgcgcct gggcaataaa tttcgtacct tccgtggtca cacggctgcg
    48961 tttatcgatc tgagcggtcc caaagatgaa gtgagcgccg cgcttgaccg cgtgatgagc
    49021 gatgagatcg tactcgatga aagcgaggcg ttttatcttc acccaggaga gctggcgctg
    49081 gcggtgacgc tggagtcggt gacgctgcca gccgatctgg tgggatggct ggacggtcgt
    49141 tcctcactgg cgcgtctggg gcttatggtg cacgtcaccg cgcaccgtat cgatccgggc
    49201 tggtctggct gtattgtgct ggagttctac aactccggta agctgccgct ggcgctgcgt
    49261 ccgggcatgt taattggtgc gctgagcttt gagccgcttt ccggcccggc ggcgcgacct
    49321 tacaaccgcc gtgaagatgc gaaatatcgc aaccagcagg gcgcggtagc cagtcgaatc
    49381 gataaagact aattcaggcc cattgaggat accatgagac gatttctgac gacgctgatg
    49441 atactcctgg tcgtgctggt ggccgggtta tctgcgttag tgttgctggt gaatccgaat
    49501 gatttccgcg actatatggt caagcaagtt gctgcacgta gcggttatca attgcagctc
    49561 gacgggccac tgcgttggca cgtctggccg cagcttagta tcctctccgg gcgaatgtct
    49621 ctcactgccc agggcgcgag ccagccactg gttcgcgcag acaacatgcg tctggacgtg
    49681 gcgcttttac cactgctgag tcatcaactg agcgttaagc aggtgatgct aaaaggggca
    49741 gtgatccaac tgacgccgca gacggaagcg gtgcgcagtg aagacgctcc ggttgcaccg
    49801 cgcgacaata ccttgccgga tctgtcagac gatcgcggat ggtcgtttga tatatccagt
    49861 cttaaggtgg cggacagcgt gctggtgttc cagcatgaag atgacgagca ggtgacaatc
    49921 cgcaatattc gcctgcaaat ggaacaagat ccccaacatc gtggttcatt tgagttctcc
    49981 gggcgggtta atcgcgatca gcgcgatctc acgatatccc ttaacggtac ggtagatgct
    50041 tctgattatc cgcatgattt aacggcggct attgaacaag ttaactggca gttgcagggt
    50101 gcagatttac caaaacaagg tattcagggg caggggagtt tccaggccca gtggcaggag
    50161 tcacataaac gcctttcatt taaccaaatc agcttgaccg ccaatgacag tacgctgagc
    50221 gggcaagcac aggtcacgct gacagagaaa ccggaatggc agctgaggct gcaattcccg
    50281 caactgaatc ttgacaacct catcccgctt aaagaaacag cgaacggtga aaacggtgcc
    50341 gcgcagcagg ggcagagcca atccacgttg ccgcgcccgg tcatttcttc gcgtattgat
    50401 gaaccggcct atcagggact gcaaggcttt acggctgata ttttgttgca ggccagtaac
    50461 gtgcgctggc gcggaatgaa ttttacagat gttgccacgc aaatgaccaa caagtcgggt
    50521 ctgctggaaa ttactcaact gcagggcaaa cttaacggtg gacaggtttc actgccgggc
    50581 acgctggacg cgacatcaat aaatccgcga ataaacttcc agccacggct ggaaaacgtt
    50641 gagattggca ccattctgaa ggcgtttaac tatccgattt cgttgaccgg aaagatgtca
    50701 ctggctggtg atttctccgg tgctgacata gatgccgacg cattccgcca taactggcaa
    50761 ggacaggcac atgtcgaaat gaccgacacg cgcatggaag ggatgaactt ccagcagatg
    50821 attcagcaag cggtagaacg taatggtggt gatgtgaagg ccgctgaaaa cttcgataac
    50881 gtaacgcgtc ttgaccgctt taccaccgat ttgacgttga aggatggcgt cgtgacgtta
    50941 aacgacatgc aaggtcaatc gcctgtgctg gcgctgacag gggaaggcat gttgaatctg
    51001 gcagatcaaa cctgcgacac ccagtttgac attcgtgtga ttggcggttg gaacggggaa
    51061 agcaaactga ttgatttcct caaagaaacg ccagtaccgc tgcgggttta tggcaactgg
    51121 cagcaactta attacagcct gcaagtggat cagttactgc gcaaacatct acaggacgaa
    51181 gcgaaacgtc gcctgaatga ctgggccgag cggaataaag attcccgcaa tggcaaagat
    51241 gtgaagaagt tgctggagaa gatgtaagac aagttatgca gatggcggcg tcaatttgtc
    51301 gccatctgta tttatagtac taagcagcat gtggatattg aacttcaata cgttgaatat
    51361 gcaatcctgc atgagccatc atggtgatca atttatcgat actaaagatc tggatcttac
    51421 ctctggcaag ttcagatatg cgaggttgag tgacccctaa agcagacgct gcctgagcct
    51481 ggctaaaccc ttgttgttca atccagttat taatgatcgt aacaagttcg gcacggatcc
    51541 gcatattttc cgcttgttca ggggtgtcac tcaccgcatc ccacacacta tcaaaggttt
    51601 caattttcat ttttccgctc ctgaacaagt tctttatagc gtcgtttggc taaatcaata
    51661 ttcgattgac tggtagtctg tgttttcttt tgaaaacaat gcaatacgta aaccgcttct
    51721 tcaaattttg cgacatacat gacgcgatag atcccatcag catcgcgagt acgaatctct
    51781 cgcacaccag ggccgatagt tgaaaaaggt ttccagtcgt atgggtttaa cccttgttgg
    51841 atcctgtcga gttgatagcc aatttcctgt cgagacgatt ctggaaatgc gcgtaaatcg
    51901 tcaagactgg tatcaagaaa agccagcttt tttctcatta ctccatcctt agattatact
    51961 aaattttgta taaatcaaat gcgcagtttg aacctgcgca tttgatgttt tatcacacct
    52021 cgtactccat ctcgccatcc ttatgcagcg gaataatctg caccttctgc acgcgatggc
    52081 tttccacctg caacgtttta agcaaataat cccccacctg aacttcttcg ccaggttttg
    52141 gaatacgttg caaatattcc atcaacagcc cggcaatggt gtgatattcg cgtttctcat
    52201 ccagcggtag cggcacatat tgcaccagat cttccagcgg catatgacca ttcgccgtcc
    52261 aggaaccgtc cgcattcttc tgaatatcat ggcgggcgtc gatctcttcc acttcgttcg
    52321 gtaagttacc ggcaatggtt tcagtgacgt cacttaatgt cacaatccct tccaccgagc
    52381 caaactcatc caccacaaaa gcaaagtgtg tgcgggcatt acggaactgc tccagggcag
    52441 gtaacaacgg caaggtttcc gggaacacca gcggctggcg aatcaacacc cgcaggttga
    52501 gcggttcgcc gcgcagagac tgttgtaaca ggtcgataac gtgaacaaca ccgagcaaat
    52561 cttctgcgtc atcgccatca gtaacgacaa gccgcgtatg ctgatttcgc tccagtaact
    52621 ggcggatttc ttcttccggc gcgttgagat cgatatgctc aatatcgtgg cgcgacgtca
    52681 taatgctgct gacggtacgc tggttaagat taagtacccg ctcaatcatc cgccgttcct
    52741 gcggattaaa gatctgctgg ttaccatgat ccaccagcat agacgcggtt tcggcgtcca
    52801 gttccgcatc ttctttttgc ccgctaagca ggcgcattac cgcttcggta gtccgctggc
    52861 gcagtgtctg gttagcggaa agaaaacgtc gccggttaaa gatagccaac tgattgagcg
    52921 cctcgatcat caccgagaaa ccaatggcag cgtacaggta gcctttcgga atgacgaagc
    52981 cgaaaccttc tgccaccagg ctaaagccaa tcatcaacag gaaacttagg cagagaataa
    53041 cgatggtcgg gtgactgtta acaaattgcg ttaacggctt gctggccatc aacatcaggc
    53101 tgatagcgat aaccacggcg gccatcatga ccagtaaatg gtcgaccatc ccgacggcgg
    53161 taatcaccga gtcgagtgag aagatggcgt ccagtaccac aatttgtgtc accacccccc
    53221 agaacttcgc acctttgcgt tgggtggggt tattgctgtc tttcccttcc agccgttcgt
    53281 tcagctccat cgtggctttg aacaacagga agaaaccacc aaacagcatg attaagtcgc
    53341 gggcgctaaa ggtaaacgag cggaagctga acagcggttg agtcagggtg accagccagg
    53401 agattgacgc cagcagtaac aggcgcatta acatcgccag cagcagcccg gtaacccgtg
    53461 cgcggtcgcg ctgcttcggc ggtagttttt cggcgaggat ggcaataaag accaggttat
    53521 caatgccgag gaccagttcg atcacaatca gcgtgattaa cccggcccag atagacggat
    53581 cggcaatcca ttccatagta agtacaaaac ctattcgttg tatgacaaat gtcacaattc
    53641 gatcatggat aaatcggggc aaaaatgcaa tgttgcagca ctatttatct catattattt
    53701 gaggtttata acctcacatt atccctgaat taaaagtggt aataataaaa cattatttaa
    53761 gaaatatcgc atgtggagac actatattaa atattgtcaa cctaaagaaa ctcctaaaaa
    53821 ccatattgaa tgacacttaa tataattctt aaaaatagcc aattgccgaa ttgttacctt
    53881 gcctgctatt ccgttagctg taacacttcc tcctgcatta ttggagagcc aatattcaat
    53941 taccataatg tcaccttata ttcggaagtt aatttccgca ataacccttt ctcatcaacg
    54001 actgtatgtt gagagggttt taaaaattaa gctaaatcag tgtattggta gctaaaaagc
    54061 caggggcggt agcgtgtctg gatgcctgaa agaccagtct gaattattct gtcaatagcc
    54121 tgcggatgaa atcaattttt ttaggactga tgccagttaa atttttattc aattaactgc
    54181 aaggtaaatg tgcatggcga cattgctatt aatagtgcac aggataatta ctctgccaaa
    54241 gtgataaata aacaatgatg aaatccaaaa tgaaattgat gccattattg gtgtcagtaa
    54301 ccttgataag cggttgcaca gtacttccgg gcagcaatat gtcgacgatg ggtaaagacg
    54361 tcatcaaaca gcaggacgct gatttcgatc tcgacaaaat ggtgaatgtt tatccgttga
    54421 ccccgcgcct gattgatcaa ttacgcccac gcccgaatgt agcgcgcccc aatatgacgc
    54481 tggaaagtga gatcgcgaat tatcagtatc gcgtcgggcc gggggacgtt cttaatgtca
    54541 ccgtctggga tcacccggaa ctcaccacgc cagccggtca gtaccgcagc tccagcgaca
    54601 ccggcaactg ggtacagcct gacggcacca tgttctaccc gtatatcggc aaggttcacg
    54661 tcgtggggaa aacgctcgct gaaatccgca gtgatattac cggtcgctta gcgacgtaca
    54721 tcgctgaccc gcaggtggac gttaatatcg ccgccttccg ctcgcaaaaa gcctatatct
    54781 caggtcaggt gaataaatcc ggtcaacagg cgattaccaa cgtaccactg accattctcg
    54841 atgccatcaa cgccgcaggt ggcctgaccg acaccgctga ctggcgcaac gtggtgctaa
    54901 cacacaatgg tcgtgaagaa cgcatttctt tgcaggcgct gatgcaaaac ggcgacctca
    54961 atcagaaccg actgctttac cccggcgata ttctctacgt gccgcgtaat gatgatctga
    55021 aagtgtttgt gatgggtgaa gttaaaaaac agagcaccct gaaaatggac tttagcggca
    55081 tgaccctaac tgaagccctg ggtaatgctg aaggcatcga catgaccacc tccaacgcca
    55141 gcggcatctt tgtcattcgt ccgttgaaag gcgagggcgg gcgtaacggc aagattgcca
    55201 atatctacca gctggatatg tccgatgcca catcgctggt gatggcgaca gaattccgcc
    55261 tgcaacctta tgacgtggtg tatgtcacca ccgccccggt ttcccgctgg aaccgtctga
    55321 tcaatcagtt gctgccaact attagcggtg tccgttacat gacggataca gccagcgaca
    55381 ttcataactg gtaatcgtca tgtttaacaa catcttagtt gtctgtgtcg gcaatatttg
    55441 ccgttccccg acggcggaac gcttactgca acgttatcac ccggagctga aagtggagtc
    55501 cgctggactc ggcgcgctgg tcggtaaggg cgctgaccct actgctatca gcgtcgccgc
    55561 agaacatcaa ctgtctctgg aaggtcactg tgcacggcaa atcagccgtc gcctgtgtcg
    55621 caactacgac ctgattttga ccatggaaaa gcgccatatc gaacgcttat gcgagatggc
    55681 accggagatg cgcggcaaag tgatgctgtt tggtcactgg gataacgaat gtgaaatccc
    55741 cgatccgtat cgcaaaagcc gggaaacgtt tgtagcggtg tacacattac ttgaacggtc
    55801 tgcccgccag tgggcgcagg cattgaacgc agagcaggta taagaatgac agaaaaagta
    55861 aaacaacatg ccgctccggt aacgggcagt gatgaaatcg atattggtcg cctggtcggc
    55921 accgtcattg aagcgcgctg gtgggtgatt ggcatcaccg ccgtatttgc cctctgtgcc
    55981 gtggtttaca ccttcttcgc tacgccgata tacagcgccg acgcactggt acaaatcgaa
    56041 caaagcagcg gcaattcgtt agtgcaggat atcggatcgg cattagccaa caaaccgcct
    56101 gcatcggacg ccgagatcca gttgattcgt tcgcgcctgg tgcttggtaa aacggtggac
    56161 gatctcgacc tcgatattgc ggtgagcaaa aacacgttcc cgattttcgg tgcgggctgg
    56221 gatcgcctga tgggacgcca gaacgagacg gtaaaagtga ccacctttaa ccgtcctaag
    56281 gagatggcgg atcaggtgtt tacgcttaat gtgcttgatg acaaaaacta cactttgagc
    56341 agcgatggcg gctttagcgc ccgtgggcaa gcgggccaga tgctgaaaaa agaaggcgtc
    56401 acgctgatgg ttgaagctat tcacgccagc ccgggcagtg agtttaccgt caccaaatac
    56461 tccacgctgg ggatgatcaa tcaactgcaa aacagcctga cggtaacgga gaacggcaaa
    56521 gatgctggcg ttttgagcct gacttatact ggagaagatc gcgaacagat ccgcgacatt
    56581 cttaacagta tcgcccgtaa ctatcaggaa caaaatattg agcgcaaatc ggcggaagcg
    56641 tcgaaaagcc tcgctttcct cgcgcaacag ttaccggaag tacgtagccg cctcgatgtt
    56701 gccgaaaaca aactgaatgc cttccgtcag gataaagatt cagttgatct gccgctggaa
    56761 gcgaaagcgg tgctcgattc gatggtgaac atcgacgcgc agttgaacga actgaccttt
    56821 aaagaagcgg aaatctccaa gctgtacacc aaagttcacc cggcgtatcg cacgctgctg
    56881 gagaaacggc aggcgctgga agacgaaaaa gccaaactta atggtcgcgt aacggcgatg
    56941 ccgaaaaccc agcaggaaat tgtccgtctg acccgcgatg tcgagtctgg gcagcaggtc
    57001 tatatgcaac tgctgaataa agagcaggag ctgaaaatca ccgaagccag caccgtcggc
    57061 gatgtgcgca ttgttgaccc ggcaatcacc cagcctggtg tgctaaaacc gaagaaaggg
    57121 ctgattatcc ttggggcgat tatccttggc ctgatgctct ctatcgtggg tgtgctgctg
    57181 cgctcgttgt ttaatcgcgg tatcgaaagt ccgcaggtgc tggaagaaca cggtatcagc
    57241 gtctatgcca gcatcccgct gtcggagtgg cagaaagcgc gcgatagcgt caaaaccatc
    57301 aaagggatta aacgctataa acagagccag ctactggcgg tggggaatcc aaccgatctg
    57361 gcgattgaag ccatccgtag tctgcgcacc agtctgcact tcgcgatgat gcaggcacag
    57421 aacaatgtgt tgatgatgac cggggttagc ccgtcaatcg gtaaaacctt tgtctgcgcc
    57481 aacctggcgg cggtgatcag tcagaccaat aaacgggtgt tgttgatcga ctgcgatatg
    57541 cgcaaaggct acacccacga gctgttgggc actaataacg tcaatggcct gtcggaaatt
    57601 ctgattggtc agggcgagat tactaccgct gctaaaccga cctctattgc caaatttgac
    57661 ctgatcccgc gcggtcaggt accgccaaat ccttcggaac tgttgatgag cgaacgtttt
    57721 gccgaactgg tgaactgggc gagtaaaaac tacgacctgg tgttgattga tacgccgccg
    57781 attctggcag tgaccgatgc ggcaattgtt ggtcgtcatg tcggaaccac gttgatggtg
    57841 gcgcgttatg cggtcaacac attgaaagaa gtggaaacca gtctgagccg ctttgagcaa
    57901 aacggtattc cggtgaaagg ggtgattctg aactccatct tccgtcgcgc cagcgcgtat
    57961 caggattatg gctattacga atacgaatat aagtcggacg cgaaataacg aaatccgctg
    58021 tcggatgcga tgctgacgca tcttatccga cctacgttca cgccgcatca ggcaagcaaa
    58081 cccgctccgt aggtcggata aggcgttcac gccgcatccg gcaacagaac ctgtaaacca
    58141 gcttataaac cggcagaaca tctcgacact ccgggagaac aacctatgaa agacaatccg
    58201 ctgatttcaa tctatatgcc gacctggaac cgtcaacaac tggcgattcg ggcgataaaa
    58261 tcggtcctgc gccaggacta cagtaactgg gagatgatca tcgtggatga ttgctccacc
    58321 tcctgggaac aactacaaca gtacgtcacc gccctcaacg atccgcgtat cacttacatt
    58381 cacaacgaca ttaacagcgg ggcgtgcgcg gtacgtaacc aggcgattat gctggcgcaa
    58441 ggggaatata tcaccgggat cgatgatgat gatgaatgga cacccaaccg tctgagcgtc
    58501 ttcctcgccc ataaacagca actggtcaca cacgcctttt tgtacgctaa cgactacgtt
    58561 tgccagggcg aagtctattc ccaaccggca agtttgccac tgtatcccaa atcgccgtat
    58621 tcacgccgcc tgttctacaa acgcaatatc attggcaatc aggtctttac ctgggcgtgg
    58681 cgtttcaaag agtgtttgtt cgataccgaa ctgaaagcgg cacaggatta cgacattttc
    58741 ctgcggatgg tggtggagta tggcgaaccg tggaaagtgg aagaggcgac gcagatcctg
    58801 catatcaatc acggcgaaat gcagatcacc tcgtcaccga aaaaattctc cggatacttc
    58861 cacttttacc gtaagcacaa agacaaattt gaccgcgcca gcaaaaaata tcagctgttt
    58921 accctctacc agatccgcaa taaacgcatg acctggcgca ccttgttgac gctgctgtcg
    58981 gtgcgtaacg gcaaacgtct ggctgatggt attcgggggc gctaaagatg ctggaagatc
    59041 tgcgcgccaa cagctggagt ttacgcccgt gctgcatggt tcttgcctat cgcgccgctc
    59101 atttttgttc ggtgtggcgc aaaaagaacg tcctcaacaa tctttgggcg gccccgctgc
    59161 tggtgctgta tcgcattatc accgaatgct ttttcggtta tgaaatccag gccgccgcga
    59221 ccattggccg ccgtttcact atccatcacg gttacgccgt agtgatcaat aaaaacgtgg
    59281 tagcggggga tgatttcacc attcgccacg gcgtcactat cggcaatcgt ggtgccgata
    59341 acatggcatg tccacacatt ggcaacggcg tcgaactcgg tgccaacgtc attattcttg
    59401 gtgatatcac gcttggtaac aacgtcaccg tgggcgcggg cagcgtggtg ctcgactctg
    59461 tcccggacaa cgcgctggtg gtgggagaaa aagcgcgagt gaaggtaatt aaatgaatat
    59521 tctgcaattc aatgtgcgac tggcggaagg cggggcggca ggtgtggcgt tagatctcca
    59581 ccagcgcgcg ctgcaacagg ggctggcgtc acattttgtc tacggctacg gcaaaggcgg
    59641 caaagagagc gtcagccatc aaaactatcc gcaggtcatc aaacatacgc cgcggatgac
    59701 cgcgatggcg aacattgcgt tgtttcgtct gcttaatcgc gatctgttcg gcaatttcaa
    59761 tgagttatat cgcaccatta ctcgcacacc gggtccggtg gtcctgcatt ttcatgtgct
    59821 gcacagctac tggttgaatc ttaagagcgt ggtgcgcttt tgcgaaaaag tgaaaaacca
    59881 taaaccggat gtcactctgg tctggacgtt acacgatcac tggagcgtta ccggacgctg
    59941 cgcctttacc gacggttgcg aaggctggaa aacgggctgc cagaaatgcc cgaccttaaa
    60001 taattatccg ccggtgaaga ttgatcgcgc acaccaacgg gtggcgggca aacggcagtt
    60061 attcagcgag atgctggcgc tgggctgtca gtttatttcc cccagccagc atgtggcaga
    60121 cgctttcaat agtctgtacg gtccagggcg ttgccggatt atcaataacg gtattgatat
    60181 ggcaaccgaa gcgattctgg cggacttgcc tccggtgcgc gaaacccagg gcaagccgaa
    60241 aatcgcggtg gtggcgcatg acctgcgtta cgacggcaaa actaaccagc aactggtacg
    60301 cgagatgatg gcgctgggcg acaaaatcga actgcatact ttcggtaagt tctcgccgtt
    60361 caccgctggt aacgttgtta atcacggctt tgaaactgac aaacgtaaac tgatgagcgc
    60421 gctcaaccag atggatgcgc tggtgttcag ttctcgcgtc gataactacc cgctgatttt
    60481 gtgtgaggcg ttatcgattg gcgtgccggt gattgccacc catagcgacg cggcgcggga
    60541 agtgctgcaa aaatccggcg gtaaaaccgt cagcgaagaa gatgtgctgc aactggtgca
    60601 gtcaagcaaa ccggaaatcg cacaggcgat atttggtacc acgctggctg agttcagcca
    60661 gcgcagccgc gccgcctaca gtggacaaca gatgctggag gagtatgtca acttctatca
    60721 gaatctgtag ctacctgctg ctgccgctga tctacctgct ggttaacgtc aaaatcgccc
    60781 agcttggcga aagttttccc atcaccatcg tcactttttt acctgtcttg ttactgctgt
    60841 ttttagaacg catcagcgtt aaaaaattga tgattgcctt agggattggt gcgggactca
    60901 cggcgtttaa ctacctgttt ggtcagtcgt tggatgccgg taaatacgtc acttcaacta
    60961 tgctgtttgt ctatattgtg atcattattg gcatggtgtg gagtattcgc tttaaaacga
    61021 tttcgcccca caaccaccgc aagatattac gtttctttta tctggtggtc gggctggtgg
    61081 tggtgctggc ggcggtggag atggcgcaaa ttatcctcac cggcggtagc agtattatgg
    61141 agtcgatttc gaaatatctg atttacagca acagctatgt gctgaatttc attaaattcg
    61201 gcggcaagcg cacgacagca ctttatttcg aaccggcatt tttcgctctg gcattaatct
    61261 caatttggct cagcatcaaa cagtttggta tcaaaacgcc taaaaccgat gctatgattc
    61321 ttgcagggat aatattatcc ggatcgtttt caggggttat gacctttatc ctgttttatt
    61381 tgctggagtg ggcatttcaa tatctgaata aagaggcgat taagaaaaag ttaccgttag
    61441 cattgatttc tctggctgta ttcctggttg gtgtggtaat cgcgtttcct tatatttcca
    61501 cccgtctggg cgatttaggt acggaaggat cgtcatcgta ttatcgtatt gtcggtccgc
    61561 tggtgatggt cggttattct ttgacccata ttgacggtgt agtcagattt ggctcacttt
    61621 atgaatatgt cgcatcattc ggaatattta acggtgcgga tgtcggaaaa accatagaca
    61681 atggtttgta tctgctgatt atttattttt cctggttcgc agtgttttta tcgctgtggt
    61741 acatggggaa agtgataaaa atgatgatca acgcttttgg tgataaccgc aattttcgcg
    61801 tgcaattata tctttttact ccggtatcgc tgttttttac cggttcgata tttagcccgg
    61861 aatatgcatt tttaatcgtc tgtccgttta ttttgcgaaa agcgttaaat attacgaggt
    61921 aagaataaga acatgttgct tagcataatc actgtcgcgt ttcgtaacct cgcagggata
    61981 gtcaaaacac atgcctcgtt ggcgcatctg gcgcaggctg acgatatcag cttcgaatgg
    62041 attgttgtcg atggcggttc caatgacggc acccgtgagt atctggaaaa tctcaatggt
    62101 atctataacc tacgctttgt cagcgagcca gataacggta tctacgacgc catgaacaaa
    62161 ggcattgcga tggcgcatgg caagttcgcg ttgtttctca actcaggcga tatttttcat
    62221 caggatgccg caaattttgt ccgtaagtta aaagtgcaaa aagataacat gatgatcacc
    62281 ggcgatgcgc tgctggattt tggcgacggg cataaaatta aacgtagcgc caaaccgggt
    62341 tggtatattt atcacagcct gcccgccagt catcaggcga tatttttccc ggtgtccggc
    62401 ttgaaaaaat ggcgttatga cctggaatat aaagtttctt ccgactacgc gctggcagcc
    62461 aaaatgtata aagccggtta tgcatttaaa aaactcaatg gcctggtgtc tgaattttct
    62521 atgggtgggg tatcaaccac caataatatg gaattgtgtg ctgacgccaa aaaagtccaa
    62581 cggcaaatat tacatgtgcc tggcttctgg gcagaattat cctggcattt acgccaacgt
    62641 actacctcaa agacgaaagc cttatataac aaaagctgaa tataagggaa aacgatgcaa
    62701 gatttaagcg gtttctcggt accgaaaggg ttccggggtg gcaacgctat taaagtgcaa
    62761 ttgtggtggg cagtacaggc aacaatattt gcctggtcgc cacaagtatt gtatcgctgg
    62821 cgggcatttt tattacgttt attcggcgca aaaataggaa aaaacgtagt tattcgtccg
    62881 tcagtaaaaa ttacctatcc gtggaaatta accttaggtg attacgcgtg ggtcggcgat
    62941 gacgtcaatt tatataccct cggtgaaata accattggcg cacattcggt gatatcgcaa
    63001 aaaagttatt tatgcaccgg tagccacgac catgcaagtc aacatttcac cattaacgcc
    63061 acgcctattg tgattggcga gaaatgctgg ctggcaaccg atgtctttgt tgccccaggc
    63121 gtcacgatcg gcaacggcac cgtcgtgggt gcacgaagca gtgtttttaa atcgcttccg
    63181 gcaaatgtgg tttgccgggg gaatcccgca gtggtgatac gcgaacgcgt tgaaactgaa
    63241 taaattcaaa aaatacagag gaataagaca tgtcaaaagt cgctctcatc accggtgtaa
    63301 ccggacaaga cggttcttac ctggcagagt ttctgctgga aaaaggttac gaggtgcatg
    63361 gtattaagcg tcgtgcatcg tcattcaaca ccgagcgcgt ggatcacatt tatcaggatc
    63421 cgcacacctg caacccgaaa ttccatctgc attatggcga cctgagtgat acctccaacc
    63481 tgacgcgtat tttgcgtgaa gtgcagccgg atgaagtgta caacctgggc gcaatgagcc
    63541 acgttgcggt ttcttttgag tcaccggaat ataccgcaga cgtcgatgcg atgggtacgc
    63601 tgcgcctgct ggaggcgatc cgcttcctcg gtctggaaaa gaaaacccgt ttctatcagg
    63661 cttctacttc tgaactgtac ggtctggtgc aggaaattcc gcagaaagag accacgccgt
    63721 tctacccacg ctctccgtat gcggtcgcca aactgtacgc ctactggatc accgttaact
    63781 accgcgaatc ctacgacatg tacgcctgta acggtattct cttcaaccat gaatccccgc
    63841 gccgtggcga gactttcgtt acccgcaaaa tcacccgcgc aatcgccaac atcgcccagg
    63901 ggctggagtc gtgcctgtat ctcggcaata tggattccct gcgtgactgg ggccacgcca
    63961 aagactacgt aaaaatgcag tggatgatgc tgcaacagga acaaccggaa gatttcgtta
    64021 tcgcgaccgg cgttcagtac tccgtacgtc agttcgtgga aatggcggca gcacagttgg
    64081 gcatcaaact gcgctttgaa ggcacgggtg ttgaagagaa gggcattgtg gtttccgtca
    64141 ccgggcatga cgcgccgggc gttaaaccgg gcgatgtgat tatcgccgtt gacccgcgtt
    64201 acttccgtcc ggcggaagtt gaaacgctgc tcggcgaccc gaccaaagcg cacgaaaaac
    64261 tgggctggaa accggaaatc accctcagag agatggtgtc tgaaatggtg gctaatgacc
    64321 tcgaagcggc gaaaaaacac tctctgctga aatctcacgg ctacgacgtg gcgatcgcgc
    64381 tggagtcata agcatgagta aacaacgcat ttttattgct ggccatcgcg ggatggtcgg
    64441 ttccgccatc aggcggcagc tcgaacagcg cggtgatgta gagctggtat tacgcacccg
    64501 cgacgagctg aacctgctgg acagccgcgc ggtgcatgat ttctttgcca gcgaaagcat
    64561 tgaccaggtc tatctggcgg cggcgaaagt gggcggcatt gttgccaaca acacgtatcc
    64621 ggcggatttc atctaccaga acatgatgat tgagagcaac atcattcacg ccgcgcatca
    64681 gaacgacgtg aacaaactgc tgtttctcgg atcgtcctgc atctacccga aactggcaaa
    64741 acagccgatg gcagaaagcg agttgttgca gggcacgctg gagccgacta acgagcctta
    64801 tgctattgcc aaaatcgccg ggatcaaact gtgcgaatct tacaatcgcc agtacggacg
    64861 cgattaccgc tcagtcatgc cgaccaacct gtacgggccg catgacaact tccacccgag
    64921 taactcccat gtgatacctg cattgctgcg tcgcttccac gaggcgacgg cacagaatgc
    64981 gccggacgtg gtggtatggg gcagcggtac accgatgcgt gaattcctgc acgtcgatga
    65041 tatggcggcg gcgagcattc atgtcatgga gctggcgcac gaagtctggc tggaaaacac
    65101 ccagccgatg ctgtcgcaca ttaacgtcgg cacgggcgtt gactgcacta tccgcgagct
    65161 ggcgcaaacc atcgccaaag tggtgggtta caaaggtcgg gtggtttttg atgccagtaa
    65221 accggatggt acaccgcgca aactgctgga tgtgacgcgc ctgcatcagc ttggctggta
    65281 tcacgaaatc tcactggaag cggggcttgc cagcacttac cagtggttcc ttgagaatca
    65341 agaccgcttt cgggggtaat gatgttttta cgtcaggaag actttgccac ggtagtgcgc
    65401 tccacaccgc ttgtctctct cgactttatt gtcgagaaca gtcgcggcga gtttctgctt
    65461 ggcaaaagaa ccaaccgccc ggcgcagggt tactggtttg tgccgggagg gcgcgtgcag
    65521 aaagacgaaa cgctggaagc cgcatttgag cggctgacga tggcggaact ggggctgcgt
    65581 ctgccgataa cagcaggcca gttttacggt gtctggcagc acttttatga cgataacttc
    65641 tccggcacgg atttcaccac tcactatgtg gtgctcggtt ttcgcttcag agtagtggaa
    65701 gaagagctgt tactgccgga tgagcagcat gacgattacc gctggctgac gccggacgca
    65761 ctgctcgcca gtgacaatgt tcacgctaac agccgcgcct attttctcgc tgagaagcgt
    65821 gccggagtac ccggattatg aaaatactgg tctacggcat taactactcg ccggagttaa
    65881 ccggcatcgg caaatacacc ggcgagatgg tggaatggct tgcggcgcaa ggtcatgagg
    65941 tgcgggttat taccgcaccg ccttactacc cgcaatggca ggtgggcgag aactattccg
    66001 cctggcggta caaacgagaa gagggggccg ccacggtgtg gcgctgcccg ctgtacgtgc
    66061 caaaacagcc gagcaccctg aaacgcctgt tgcatctcgg cagttttgcc gtcagcagtt
    66121 ttttcccgtt gatggcgcaa cgtcgctggc agccggatcg cattattggc gtggtgccaa
    66181 cgctgttttg cacgccggga atgcgcctgc tggcgaaact ctctggggcg cgcacggtgc
    66241 tccatattca ggattacgaa gtggacgcca tgctggggct gggccttgcc ggaaaaggta
    66301 aaggcggcaa agtggcacag ctggcaacgg cgttcgaacg tagcggactg cataacgtcg
    66361 ataacgtctc cacgatttcg cgttcgatga tgaataaagc tatcgaaaaa ggcgtggcgg
    66421 cggaaaacgt catcttcttc cccaactggt cggaaatcgc ccgttttcag catgttgcaa
    66481 atgccgatgt tgatgccctt cgtaaccagc ttggcctgcc ggataacaaa aaaatcattc
    66541 tttactccgg caatattggt gaaaagcagg ggctggaaaa cgttattgaa gctgccgatc
    66601 gcctgcgcga tgaaccgctg atttttgcca ttgtcgggca gggcggcggc aaagcgcggc
    66661 tggaaaaaat ggcgcagcag cgtggactgc gcaacatgca attttttccg ctgcaatcgt
    66721 atgacgcttt acccgcactg ctgaagatgg gcgattgcca tctggtggtg caaaaacgcg
    66781 gcgcggcaga tgccgtattg ccgtcgaaac tgaccaatat tctggcggta ggcggtaacg
    66841 cggtgattac cgcagaagcc cacaccgaac tgggacagct ttgcgaaacc tttccgggca
    66901 ttgcggtttg cgtggaaccg gaatcggtcg aggcgctggt ggcggggatt cgtcaggcgc
    66961 tcctgctgcc caaacacaac acggtggcac gtgaatatgc cgaacgcacg ctcgataaag
    67021 agaacgtgtt acgtcaattt ataaatgata ttcggggata attatggcgc agtcgaaact
    67081 ctatccagtt gtgatggcag gtggctccgg tagccgctta tggccgcttt cccgcgtact
    67141 ttaccccaag cagtttttat gcctgaaagg cgatctcacc atgctgcaaa ccaccatctg
    67201 ccgcctgaac ggcgtggagt gcgaaagccc ggtggtgatt tgcaatgagc agcaccgctt
    67261 tattgtcgcg gaacagctgc gtcaactgaa caaactcacc gagaatatta ttctcgaacc
    67321 ggcagggcgt aacactgcac ctgccattgc gctggcggcg ctggcggcaa aacgtcatag
    67381 cccggagagc gacccattaa tgctggtgct ggcggcggat catgtgattg ccgatgaaga
    67441 cgcttttcgc accgccgtgc gtaatgccat gccgtatgcc gaagcgggca agctggtgac
    67501 cttcggcatt gtgccggatc tacctgaaac cggatatggc tatattcgtc gcggtgaagt
    67561 gtctgcgggt gagcaggata cggtggcctt tgaagtggcg cagtttgtcg aaaaaccgaa
    67621 tctggaaacc gcgcaggcct atgtggcaag cggcgaatat tactggaaca gcggtatgtt
    67681 tctgttccgc gccggacgct atctcgaaga actgaaaaaa tatcgcccgg atattctcga
    67741 cgcctgtgaa aaagcgatga gcgccatcga tccggatctc gattttattc gtgtggatga
    67801 agaagcgttt ctcgcctgcc cggaagagtc ggtggattac gcagtcatgg aacgtacggc
    67861 agatgctgtt gtggtgccga tggatgcggg ctggagcgat gtcggttcct ggtcctcttt
    67921 atgggagatc agcgcccaca ccgccgaggg caacgtttgc cacggcgatg tgattaatca
    67981 caaaactgaa aacagctatg tgtatgccga atctggcctg gtcaccaccg tcggggtgaa
    68041 agatctggtg gtggtgcaga ccaaagatgc ggtgctgatt gccgaccgta acgcggtgca
    68101 ggatgtgaaa aaagtggtcg agcagatcaa agccgatggt cgccatgagc atcgggtgca
    68161 tcgcgaagtg tatcgtccgt ggggcaaata tgactctatc gacgcgggcg accgctacca
    68221 ggtgaaacgc atcaccgtga aaccgggcga aggcttgtca gtacagatgc accatcaccg
    68281 cgcggaacac tgggtagtgg tcgcgggaac ggcaaaagtc actatcgacg gtgatatcaa
    68341 actccttggt gaaaacgagt ccatttatat tccgctgggg gcgacgcatt gcctggaaaa
    68401 cccggggaaa attccgctcg atttaattga agtgcgctcc ggctcttatc ttgaagagga
    68461 tgatgtggtg cgcttcgcgg atcgctacgg acgggtgtaa acgtcgcatc aggcgctgtt
    68521 tgtcggatgc ggcgtgaacg ccttatccga cctacggttc gggtttgtag gcctgataag
    68581 acgcggcagc gtcgcatcag gcaatgaatg cgaaaccgcg atgtaaataa cgacaaaaac
    68641 aaaattggcc gtttcggtca gggccaacta ttgcctgaaa aagggtaacg atatgaaaaa
    68701 attaacctgc tttaaagcct atgatattcg cgggaaatta ggcgaagaac tgaatgaaga
    68761 tatcgcctgg cgcattggtc gcgcctatgg cgaatttctc aaaccgaaaa ccattgtgtt
    68821 aggtggtgat gcccgcctca ccagcgaaac cttaaaactg gcgctggcga aaggtttaca
    68881 ggatgcgggc gtcgatgtgc tggatattgg tatgtccggc accgaagaaa tctatttcgc
    68941 cacgttccat ctcggcgtgg atggcggcat tgaagttacc gccagccata acccgatgga
    69001 ttacaacggt atgaaactcg tgcgcgaggg ggcccgcccg atcagcgggg ataccggact
    69061 gcgcgacgtc cagcgtctgg cagaagccaa cgactttcct cccgttgatg aaaccaaacg
    69121 cggtcgctat cagcaaatca atctgcgtga cgcttacgtt gatcacctgt tcggttatat
    69181 caacgtcaaa aacctcacgc cgctcaagct ggtgatcaac tccgggaacg gcgcagcggg
    69241 tccggtggtg gacgccattg aagcccgctt taaagccctc ggcgcaccag tggaattgat
    69301 caaagtgcac aacacgccgg acggcaattt ccccaacgga attcctaacc cgctgctgcc
    69361 ggaatgccgc gacgacaccc gcaatgcggt catcaaacac ggcgcggata tgggcattgc
    69421 ctttgatggc gattttgacc gctgttttct gtttgacgaa aaagggcagt ttatcgaggg
    69481 ctactacatt gtcggcctgc tggcagaagc gttcctcgaa aataatcccg gcgcgaagat
    69541 catccacgat ccgcgtctct cctggaacac cgttgatgtg gtgactgccg caggcggcac
    69601 cccggtaatg tcgaaaaccg gacacgcctt tattaaagaa cggatgcgca aggaagacgc
    69661 catctacggt ggcgaaatga gcgcccacca ttacttccgt gatttcgctt actgcgacag
    69721 cggcatgatc ccgtggctgc tggtcgccga actggtgtgc ctgaaaggga aaacgctggg
    69781 cgaactggtg cgtgaccgga tggcagcatt tccggcaagc ggtgagatca acagcaaact
    69841 ggcggaccct gttgaggcga ttaaccgcgt cgaacagcat tttagccgcg aggcgctggc
    69901 ggtggatcgc accgatggca tcagcatgac ctttgccgac tggcgcttta acctgcgcac
    69961 ctccaacacc gaaccggtgg tgcgcctgaa tgttgaatcg cgcggtgatg tgccgctgat
    70021 ggaagcgcga acgcgaactc tgctgacgtt gctgaacgag taatgtcgga tcttccctta
    70081 ccccactgcg ggtaaggggc taataacagg aacaacgatg acaaatctaa aaaagcgcga
    70141 gcgagcgaaa accaatgcat cgttaatctc tatggtgcaa cgcttttcag atatcaccat
    70201 catgtttgcc ggactatggc tggtttgcga agtcagcgga ctgtcattcc tctacatgca
    70261 cctgttggtg gcgctgatta cgctggtggt gttccagatg ctgggcggca tcaccgattt
    70321 ttatcgctca tggcgcggtg ttcgggcagc gacagaattt gccctgctgc tacaaaactg
    70381 gaccttaagc gtgattttca gcgccgggct ggtggcgttc aacaatgatt tcgacacgca
    70441 actgaaaatc tggctggcgt ggtatgggct gaccagtatc ggactggtgg tttgccgttc
    70501 gtgtattcgc attggggcgg gctggctgcg taatcatggc tataacaagc gcatggtcgc
    70561 ggtggcgggg gatttagccg ccggacaaat gctgatggag agcttccgta atcagccgtg
    70621 gttagggttt gaagtggtgg gcgtttacca cgacccgaaa ccgggtggcg tttctaacga
    70681 ctgggcgggc aatctgcaac agctggtcga ggatgcgaaa gcgggcaaga ttcataacgt
    70741 ctatatcgcg atgcaaatgt gcgacggcgc acgcgtgaaa aaactggtcc atcaattggc
    70801 ggacaccacc tgttcggtgc tgctgatccc cgatgtcttt accttcaaca ttctccattc
    70861 ccgtatcgaa gagatgaacg gcgtaccggt ggtgccgctg tatgacacgc cgctttccgg
    70921 ggttaaccgc ctgctcaaac gtgcggaaga tattgtgctg gcgacgctta tcctgctgct
    70981 gatctccccg gtgctgtgct gcattgcgct ggcggtgaaa ctgagctcac cggggccggt
    71041 tattttccgc cagactcgct acggcatgga cggcaagccg atcaaagtgt ggaagttccg
    71101 ttccatgaaa gtgatggaga acgacaaagt ggtgacccag gcgacgcaga acgatccgcg
    71161 cgtcaccaaa gtggggaact ttctgcgccg cacctcgctg gatgaattgc cgcagtttat
    71221 caatgtgctg actggcggga tgtcgattgt cgggccacgt ccgcacgcgg tggcgcataa
    71281 cgaacagtat cgccagctca ttgaaggcta catgctgcgc cataaagtga aaccgggcat
    71341 taccggctgg gcgcagatta acggctggcg cggcgaaacc gacacactgg agaaaatgga
    71401 aaaacgcgtc gagttcgacc ttgagtacat ccgcgaatgg agcgtctggt tcgatatcaa
    71461 aatcgttttc ctgacggtat tcaagggctt cgttaacaaa gcggcatatt gatatgaact
    71521 tacgtgaaaa aaccatcagc ggcgcgaagt ggtcggcgat tgccacggtg atcatcatcg
    71581 gcctcgggct ggtgcagatg accgtgctgg cgcggattat cgacaaccac cagttcggtt
    71641 tgcttaccgt gtcgctggtg atcatcgcgc tggcagatac gctttctgac ttcggcatcg
    71701 ctaactcgat tattcagcga aaagaaatca gccaccttga actcaccacg ctgtactggc
    71761 tgaacgtcgg gctggggatc gtggtgtgcg tggcggtgtt tttgttgagt gataccattg
    71821 gcgacgtgct gaataacccg gacctggcac cgttgattaa aacattgtcg ctggcgtttg
    71881 tggtgatccc ccacgggcag cagttccgcg cgttgatgca gaaggagctg gagttcaaca
    71941 aaatcggcat gatcgaaacc agtgcggtgc tggcgggctt cacttttacg gtggttagcg
    72001 cccatttctg gccgctggcg atgaccgcga tcctcggtta tctggtcaat agtgcggtaa
    72061 gaacgctgct gtttggctac tttggccgca aaatttatcg ccccggtctg catttctcgc
    72121 tggcgtcggt ggcaccaaac ttacgttttg gtgcctggct gacggcggac agcatcatca
    72181 actatctcaa taccaacctt tcaacactcg tgctggcgcg tattctcggc gcgggcgtgg
    72241 cagggggata caacctggcg tacaacgtgg ccgttgtgcc gccgatgaaa ctgaacccca
    72301 tcatcacgcg cgtgttgttt ccggcatttg ccaaaattca ggacgacacc gaaaagctgc
    72361 gcgtcaactt ctacaagctg ctgtcggtgg tggggattat caactttccg gcgctgctgg
    72421 ggctgatagt ggtgtcgaat aactttgtac cgctggtatt tggtgagaag tggaacagca
    72481 ttattccggt gctgcaattg ctgtgtgtgg tggggctgct gcgctcagtg gggaacccga
    72541 ttggttcgct gctgatggcg aaagcgcggg tcgatatcag ctttaaattc aacgtattca
    72601 aaacctttct gtttattccg gcgattgtta ttggtgggca gatggcgggc gcaatcggcg
    72661 tcacgcttgg cttcctgctg gtgcagatta tcaacaccat tctgagctat ttcgtgatga
    72721 ttaaaccggt gctcggttcc agttatcgcc agtacatcct gagtttgtgg ctgccgtttt
    72781 atctctcgct gccgacgctg gtggtcagtt atgcgctggg cattgtgctg aaagggcaac
    72841 tggcgctggg gatgctgctg gcggtgcaaa tagccacggg cgtgctggcg tttgtggtga
    72901 tgattgtgct gtcgcgccat ccgctggtgg tggaagtgaa gcgtcagttt tgtcgcagcg
    72961 aaaaaatgaa aatgctttta cgggcggggt gaatggttgt tttcggttcc ggcaacggtt
    73021 gccggatgcg gcgtaaaacg ccttatccgt cctggggggc tgggcttgga tcttgtaggc
    73081 ctgataagac gcgccagcgt cgcatcaggc actgaatgcc ggatgcggcg tgaacgcctt
    73141 atccggccta cggttctgtg ctcggatctt gtaggcctga taagacgcgt cagcgtcgca
    73201 tcaggcactg aatgccggat gcggcgtaaa acgccttatc cgtcctacgg ctctgtgctc
    73261 gggtcttgta ggcctgataa gacgcgtcag cgtcgcatca ggcactgaat gccggaagcg
    73321 gcgtgaacgc cttatccgtc ctacggttct gggcttggat cttgtaggcc tgataagagg
    73381 cgtcagcgtc gcatcaggta ctgaatgccg gatgcggcgt gaacgcctta tccgtcctac
    73441 ggttctgggc ttggatcttg taggcctgat aagacgcgtc agcgtcgcat caggcactga
    73501 atgccggatg cggcgtgaac gccttatccg tcctacggtt ctgtgctcgg atcttgtagg
    73561 cctgataaga cgcgttagcg tcgcatcagg caaaaaacgg caacgaacac aacaaacaca
    73621 gcctcacctg gcatcaaatt tatgaggaaa aaatgaaatt acttattctg ggcaaccaca
    73681 cttgcggcaa tcgtggcgat agcgccatcc tgcgtggctt acttgatgcc atcaatatcc
    73741 tcaatccaca tgccgaagtg gacgtgatga gccgctatcc ggtcagttct tcctggctgc
    73801 tcaaccgccc ggtaatgggc gatccgctgt tcctgcaaat gaaacaacac aacagcgcgg
    73861 cgggcgttgt cgggcgtgtt aaaaaagtcc tccgtcgccg ctaccagcac caggtattgc
    73921 tctcacgcgt caccgacact ggcaagctgc gtaatatcgc catcgcccag ggattcactg
    73981 acttcgtgcg actgctgtca ggttacgacg ccattattca ggtcggcgga tcgttttttg
    74041 tcgatctcta cggcgtgccg cagtttgaac atgcgctttg cactttcatg gcgaaaaagc
    74101 cgctgtttat gattggtcac agcgtcggcc ccttccagga tgagcaattt aaccaactgg
    74161 cgaactatgt ctttggtcac tgcgacgcgc tgatcctgcg cgaatcggtc agccttgatt
    74221 tgatgaaacg cagcaatatc accactgcaa aagtggaaca tggcgtcgat accgcgtggc
    74281 tggtcgatca ccacattgaa gacttcaccg ccagctatgc tgttcaacac tggctggatg
    74341 ttgccgcaca acagaaaacg gtggcaatta ccctgcgcga actggcaccg ttcgacaaac
    74401 gtctcggcac cactcaacaa gcgtatgaaa aagcctttgc cggggtggtc aatcgcattc
    74461 tcgatgaagg gtatcaggtg attgcgctct ccacctgtac gggcatcgac agttataaca
    74521 aagacgaccg catggtggcg ctcaatctgc gccagcacat cagcgatcct gcccgttatc
    74581 acgtagtgat ggatgaactc aacgatctgg aaatgggcaa aattctgggg gcctgtgaac
    74641 ttaccgtcgg tacgcgcctg cactctgcta ttatctcgat gaactttgcc actccggcga
    74701 ttgccatcaa ctacgaacat aaatccgccg ggatcatgca acaactgggg ctaccagaaa
    74761 tggcgattga tatccgtcat ttattagacg gcagcctgca agcgatggtt gcggatacct
    74821 taggccagct tccggcactg aacacgcgac ttaacgaagc cgtcagtcgc gaacgtcaga
    74881 cgggaatgca gatggtgcaa tctgtgcttg aacgcatcgg ggaggtgaaa tgaaggtcgg
    74941 cttcttttta ctgaaatttc cgttgtcgtc ggaaaccttc gttcttaacc aaatcactgc
    75001 gtttattgat atgggatttg aggtggagat tatcgcgctg caaaaaggcg acacacaaaa
    75061 cacccacgcg gcatggacga aatacaacct tgctgccaga acccgctggt tacaggacga
    75121 acctacgggc aaagtggcga aactgcgcca ccgcgccagc cagaccttat gcggcattca
    75181 tcgtaaaaat acctggcagg cgctcaacct caaacgctat ggcgccgagt cgcggaacct
    75241 gattttgtct gctatttgcg gtcaggtcgc aacaccgttt catgccgatg tctttatcgc
    75301 tcactttggt ccggcagggg taaccgcagc aaaactccgc gaactgggtg tcattcgcgg
    75361 caaaattgcc actatcttcc acggtattga tatctccagt cgggaggtgc tcaaccatta
    75421 cactcccgaa tatcagcaac tgttccgccg tggcgacctg atgttaccga taagcgatct
    75481 gtgggccgga aggctgcaaa aaatgggctg cccgagggaa aaaatcgccg tatcgcgtat
    75541 gggcgtggac atgacgcgct ttagcccgcg tccggtgaaa gcgcctgcaa cgccgctgga
    75601 aatcatttcc gtcgcacgct taaccgagaa aaaaggcctg catgtggcga tcgaagcctg
    75661 ccgtcagttg aaagagcagg gcgtggcatt tcgctatcgc atcctcggca ttggcccgtg
    75721 ggaacgacgc ctgcgcaccc tcatcgaaca atatcaactg gaagatgtga tagagatgcc
    75781 gggctttaaa ccgagccatg aagtgaaggc gatgctcgac gacgcggatg tcttcctgtt
    75841 gccatcggtt acaggtgcgg acggcgatat ggaaggtatt ccggtggcac taatggaagc
    75901 gatggcggtc ggcattccgg tggtttctac tctgcatagc ggaataccgg aactggtgga
    75961 ggccgataaa tccggctggc tggtgcctga gaacgatgct cgcgcactgg cgcaacgact
    76021 ggcggcgttt agccaactgg acaccgacga actggctccg gttatcaaac gtgctcgcga
    76081 aaaagtcgaa cacgatttta accagcaggt gatcaatcga gaactcgcca gcttgctgca
    76141 ggctttatag aggagaacgt atgccattta aaaaactctc ccgacgcacc ttcctgacgg
    76201 caagctcggc gcttgccttc ctccataccc ccttcgcccg cgcacttcca gcccgacaaa
    76261 gcgttaatat caacgattac aatccgcacg actggatcgc ctcatttaaa caagccttca
    76321 gtgaagggca aacggtcgtc gtgcctgccg gattggtttg tgacaatatc aacaccggca
    76381 tcttcattcc tcctggaaaa accttacaca tccttggaag cctgcgcggc aacggcagag
    76441 ggcgatttgt cttacaggac ggcagccagg tgacagggga ggagggcggc agtatgcata
    76501 acatcaccct ggatgtgcgc ggttctgact gcatcatcaa agggttggcg atgagcggtt
    76561 ttggcccggt aacgcagatt tatatcggcg gcaaaaataa acgcgtcatg cgcaacttga
    76621 ccatcgataa cctgaccgtc agccacgcta attacgccat tttacgccag ggatttcata
    76681 accagattat cggtgccaac atcaccaact gtaagttcag cgacttacag ggcgacgcca
    76741 ttgaatggaa tgtggcgatt aacgaccgcg atattttgat atccgaccac gttattgagc
    76801 gcatcaactg taccaacggc aaaatcaact ggggcatcgg cataggcctt gcgggaagca
    76861 cttacgataa caactatccg gaagaccagg cagtgaaaaa ctttgtcgtg gcgaatatca
    76921 cgggatcaga ttgtcgacag ttgatacatg ttgaaaatgg taaacatttt gttattcgta
    76981 atatcaaagc ccgcaatatc acgccggatt tcagtaagaa agcaggtatt gataacgcca
    77041 cggtcgctat ttacggttgt gacaatttcg tgattgataa tattgaaatg attaatagcg
    77101 ccgggatgtt aattggctat ggggtaatta aaggcaaata tctctcgata ccgcaaaatt
    77161 tccgagtgaa taatattcaa ctggataaca ctcaccttgc ttataaattg cgcggcatcc
    77221 aaatctccgc cgggaatgct gtctcctttg tggcgctgac taacattgag atgaagcgtg
    77281 cctcgctgga gcttcacaat aaaccgcaac atctttttat gcgaaatatc aaagtgatgc
    77341 aggaatcctc tgttggaccc gcattgagca tgaacttcga catgcgcaaa gacgttcgcg
    77401 gcgtctttat ggcgaaaaaa gaaacactgc tgtctcttgc aaatgttcat gcgatgaatg
    77461 aaagagggca aagctccgtc gatatcgaca gggttaatca ccatattgtt aatgtggaaa
    77521 agattaactt tagattgcca gaacggaggg agtagatttg cgaccattca taaacttact
    77581 ggatataatg ttctaaaaga ttataatcct cacactaact gttaaagctg gggctgggca
    77641 gcattgacta ctcagggaag gtactcatct ttatcttgtg tttcttacgt aacaaccaga
    77701 ctaaagtgat ttatgaacga taacgttttg ctcataggag cttccggatt cgtaggaacc
    77761 cgactacttg aaacggcaat tgctgacttt aatatcaaga acctggacaa acagcagagc
    77821 cacttttatc cagaaatcac acagattggc gatgttcgtg atcaacaggc actcgaccag
    77881 gcgttagctg gttttgacac tgttgtgcta ctggcagcgg aacaccgcga tgacgtcagc
    77941 cctacttctc tctattatga tgtcaacgtt cagggtaccc gcaatgtgct gacggccatg
    78001 gaaaaaaatg gcgttaaaaa tatcatcttt accagttccg ttgctgttta tggtttgaac
    78061 aaacacaacc ctgacgaaaa ccatccacac gatcctttca accactacgg caaaagtaag
    78121 tggcaggcag aggaagtgct gcgtgaatgg tataacaaag caccaacaga acgttcatta
    78181 accatcatcc gtcctaccgt tatcttcggt gaacgcaacc gcggtaacgt ctataacttg
    78241 ctgaaacaga tcgctggcgg caagtttatg atggtgggcg cagggactaa ctataagtcc
    78301 atggcttatg ttggaaacat tgttgagttt atcaagtaca aactgaagaa tgttgccgca
    78361 ggttatgagg tttataatta cgttgataag ccagatctga acatgaacca gttggttgct
    78421 gaagttgaac aaagtctgaa caaaaagatc ccttctatgc acttgcctta cccactagga
    78481 atgttgggtg gatattgctt tgatatcctg agcaaaatta cgggcaaaaa atacgctgtc
    78541 agctctgtgc gcgtgaaaaa attctgcgca acaacacagt ttgacgcaac gaaagtgcat
    78601 tcttcaggtt ttgtggcacc gtatacgctg tcgcaaggtc tggatcgaac actgcagtat
    78661 gaattcgttc atgccaaaaa agacgacata acgtttgttt ctgagtaatg cagacgagtc
    78721 agagaaaaaa ggaccaggtt ggactacgat tattcggaaa gaggtactga ttaaattatc
    78781 agtatgcgga attccgagta atccgagtaa cccagatgct gatggtgtgt agtgttattc
    78841 taaggtttaa gcaaagttat gcggaactaa gctttggtat atagccataa cgtttggcta
    78901 acctatggca ataatttacg cactatcagc cacttagagg taggaaaaga atgacgaatt
    78961 taaaagcagt tataccggta gcaggtcttg ggatgcatat gttgcctgcc actaaggcga
    79021 ttcccaaaga gatgctaccg atcgtcgaca agccaatgat tcagtacatt gttgacgaga
    79081 ttgtggctgc agggatcaaa gaaatcctcc tggtaactca cgcgtccaag aacgcggtcg
    79141 aaaaccactt cgacacctct tatgaattag aatctctcct tgagcagcgc gtgaagcgtc
    79201 aactactggc ggaagtgcaa tctatctgcc cgccgggcgt gactattatg aacgtgcgtc
    79261 agggcgaacc tttaggttta ggtcactcca ttttgtgtgc acgccccgcc attggtgaca
    79321 acccatttgt cgtggtgctg ccggacgttg ttatcgatga tgccagcgcc gacccgctgc
    79381 gttacaacct tgccgctatg attgcgcgct tcaacgaaac gggccgcagc caggtgctgg
    79441 caaaacgtat gccgggtgac ctctctgaat actccgtcat ccagaccaaa gaaccgctgg
    79501 atcgtgaagg caaagtcagc cgcattgttg aattcatcga aaaaccggat cagccgcaga
    79561 cgctggactc agacatcatg gccgttggtc gctatgtgct ttctgccgat atttggccgg
    79621 aactggaacg cactcagcct ggtgcatggg ggcgtattca gctgactgat gctattgccg
    79681 aactggcgaa aaaacagtcc gttgatgcca tgctgatgac tggtgacagc tacgactgtg
    79741 gtaaaaaaat gggctatatg caggcgtttg tgaagtatgg actgcgcaac ctgaaagaag
    79801 gggcgaagtt ccgtaaaggt attgagaagc tgttaagcga ataatgaaaa tctgaccgga
    79861 tgtaacggtt gataagaaaa ttataacggc agtgaagatt cgtggcgaaa gtaatttgtt
    79921 gcgaatattc ctgccgttgt tttatataaa caatcagaat aacaaagagt tagcaatagg
    79981 attttcgtca aagttttcca ggattttcct tgtttccaga gcggattggt aagacaatta
    80041 gcgtttgaat ttttcgagtt aagcgcgagt gggtaacgct cgtcacatcg taggcatgca
    80101 tgcagtgctc tggtagctgt aaagccaggg gcggtagcgt gcattaatac ctctattaat
    80161 caaactaaga gccgcttatt tcacagcatg ctctgaagta atatggaata aaagagtgaa
    80221 gatacttgtt actggtggcg caggatttat tggttctgct gtagttcgtc acattataaa
    80281 taatacgcag gatagtgttg ttaatgtcga taaattaacg tacgccggaa acctggaatc
    80341 acttgctgat gtttctgatt ctgaacgcta tatttttgaa catgcggata tttgcgatgc
    80401 tgctgcaatg gcgcggattt ttgctcagca tcagccggat gcagtgatgc acctggctgc
    80461 tgaaagccat gtggatcgtt caattacagg ccctgcggca tttattgaaa ccaatattgt
    80521 tggtacttat gtccttttgg aagccgctcg caattattgg tctgctcttg atggcgacaa
    80581 gaaaaatagc ttccgttttc atcatatttc tactgacgaa gtctatggtg atttgcctca
    80641 tcctgacgaa gtaaataata aagaacaatt acccctcttt actgagacga cagcttacgc
    80701 gcctagtagt ccttattccg catcaaaagc atccagcgat catttagtcc gtgcgtggaa
    80761 acgtacctat ggtttaccga ccattgtgac taattgttcg aataactacg gtccttatca
    80821 ctttccggaa aaattgattc cactagtaat tcttaatgct ctggaaggta aggcattacc
    80881 tatttatggc aaaggggatc aaattcgcga ctggctgtat gttgaagatc atgcacgtgc
    80941 gttatatacc gtcgtaaccg aaggtaaagc gggtgaaact tataacattg gtggacacaa
    81001 cgaaaagaaa aacatcgatg tagtgctcac tatttgtgat ttgttggatg agattgtacc
    81061 gaaagagaaa tcttaccgcg agcaaattac ttatgttgcc gatcgtccgg gacacgatcg
    81121 ccgttatgcc attgatgctg agaagattgg tcgcgaattg ggatggaaac cacaggaaac
    81181 gtttgagagc gggattcgga agacagtgga atggtacctg tccaatacaa aatgggttga
    81241 taatgtgaaa agtggtgcct atcaatcgtg gattgaacag aactatgagg gccgccagta
    81301 atgaatattc tccttttcgg caaaacaggg caggtaggtt gggaactaca gcgtgctctg
    81361 gcacctttgg gtaatttgat tgctcttgat gttcactcca ctgattattg tggtgatttt
    81421 agtaatcctg aaggtgtggc tgaaaccgtc aaaaaaattc gccctgatgt tattgttaat
    81481 gctgcggctc ataccgcagt agataaggct gagtcagaac ccgaatttgc acaattactc
    81541 aatgcgacca gcgttgaatc aattgcaaaa gcggctaatg aagttggggc ctgggtaatt
    81601 cattactcaa ctgactacgt atttccggga accggtgaaa taccatggct ggagacggat
    81661 gcaaccgcac cgctaaatgt ttacggtgaa accaagttag ctggagaaaa agcattacaa
    81721 gagcagtgtg cgaagcacct tattttccgt accagctggg tatatgcagg taaaggaaac
    81781 aattttgcca agacaatgtt acgtctggca aaagagcgtg aagaattagc cgttattaat
    81841 gatcagtttg gtgcgccaac tggcgcagag ttgctggctg attgtacggc acatgccatt
    81901 cgtgtggcac tgaataaacc ggaagtcgca ggtttgtacc atctggtagc cagtggtacc
    81961 acaacctggc acgattatgc tgcgctggtt tttgaagagg cgcgcaaagc aggcataccc
    82021 cttgcactca acaagctcaa cgcagtagca acaacagcct atcctacacc agctcgtcgt
    82081 ccacataact ctcgccttaa tacagaaaaa tttcagcaga attttgcgct tgttttgcct
    82141 gactggcagg ttggcgtgaa acgaatgctc aacgaattat ttacgactac agtaatttaa
    82201 tagtttttgc atcttgttcg tgatgatgga gcaagatgaa ttaaaaggaa tgatgaaatg
    82261 aaaacgcgta aaggtattat tttagcgggt ggctctggta ctcgtcttta tcctgtgact
    82321 atggctgtca gtaaacagct gttacctatt tatgataaac cgatgatcta ttacccgctt
    82381 tctacactga tgttagcggg tattcgcgat attctgatta tcagtacacc acaggatact
    82441 cctcgttttc accaactgct gggtgacggg ggccagtggg ggctaaatct tcagtacaaa
    82501 gtgcaaccga ctccagatgg gcttgcgcag gcgtttatta tcggtgaaga gtttatcggt
    82561 ggtgatgatt gtgctctggt tctcggtgat aatatctttt acggtcatga tctgccgaag
    82621 ttaatggatg tcgctgttaa caaagaaagt ggtgcaacag tatttgccta tcatgttaat
    82681 gatcctgaac gctacggtgt cgttgagttt gataaaaaag gtacggcaat tagcctggaa
    82741 gaaaaaccgt tacaaccaaa aagtaattat gcggtaaccg ggctttattt ctatgataac
    82801 gacgttgtcg aaatggcgaa aaaccttaag ccttctgccc gtggtgaact ggaaattacc
    82861 gatattaacc gtatttatat ggaacagggg gggttatccg ttgccatgat gggacgtggt
    82921 tatgcatggc tggacacggg tacacatcaa agtcttattg aagcaagcaa cttcattgca
    82981 acaattgaag agcgtcaggg gttaaaagtt tcctgtccgg aagaaattgc ttaccgtaaa
    83041 gggtttattg atgctgagca ggtgaaagta ttagctgaac cgctgaaaaa aaatgcttat
    83101 ggtcagtatc tgctgagaat gattaaaggt tattaataaa atgaacgtaa ttaaaacaga
    83161 aattccagaa gtcttgattt ttgagcctaa aaaatttgag gactccaggg ggtatttttt
    83221 tgaaagcttt aatcaaa
//
LOCUS       DAZGQL010000007        76290 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.007, whole genome shotgun sequence.
ACCESSION   DAZGQL010000007 DAZGQL010000000
VERSION     DAZGQL010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 76290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 76290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..76290
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.007"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(69..548)
                     /gene="folK"
                     /locus_tag="ACHKIR_000570"
     CDS             complement(69..548)
                     /gene="folK"
                     /locus_tag="ACHKIR_000570"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFO9988361.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESRILAVSSFYRTP
                     PLGPQDQPDYLNAAVALKTTLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELLFPDGLSLVEALQAKGFNELDKW"
     gene            complement(545..1942)
                     /gene="pcnB"
                     /locus_tag="ACHKIR_000571"
     CDS             complement(545..1942)
                     /gene="pcnB"
                     /locus_tag="ACHKIR_000571"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HFO9988362.1"
                     /translation="MFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQHAISRKDI
                     SENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLV
                     GRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGSIEEDAQR
                     RDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLRAVRFAAK
                     LGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEYHLFQPLF
                     PTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLLETAQKIA
                     QESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLL
                     EHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDEEPSPRRR
                     TRRPRKRAPRREGTA"
     gene            complement(2002..2898)
                     /gene="gluQRS"
                     /locus_tag="ACHKIR_000572"
     CDS             complement(2002..2898)
                     /gene="gluQRS"
                     /locus_tag="ACHKIR_000572"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HFO9988363.1"
                     /translation="MTDTHYIGRFAPSPSGELHFGSLIAALGSYLQARARQGRWLVRI
                     EDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWLHEQGLSYYCT
                     CTRARIQSIGGIYDGHCRELHHGPDNAAVRIRQQHPVTLFTDQLRGIIHANEKLARED
                     FIIHRRDGLFAYNLAVVVDDHFQGVSEIVRGADLIEPTVRQISLYQLFGWKVPDYIHL
                     PLALNTQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFSVEQILQSAVK
                     NWRLTAVPESAIVNSTFSNASC"
     gene            complement(2965..3420)
                     /gene="dksA"
                     /locus_tag="ACHKIR_000573"
     CDS             complement(2965..3420)
                     /gene="dksA"
                     /locus_tag="ACHKIR_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HFO9988364.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3598..4302)
                     /gene="sfsA"
                     /locus_tag="ACHKIR_000574"
     CDS             complement(3598..4302)
                     /gene="sfsA"
                     /locus_tag="ACHKIR_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFO9988365.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTSC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     LSAEGMALKKSLPVTL"
     gene            complement(4317..4847)
                     /gene="thpR"
                     /locus_tag="ACHKIR_000575"
     CDS             complement(4317..4847)
                     /gene="thpR"
                     /locus_tag="ACHKIR_000575"
                     /EC_number="3.1.4.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HFO9988366.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPDAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYTVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4921..7350
                     /gene="hrpB"
                     /locus_tag="ACHKIR_000576"
     CDS             4921..7350
                     /gene="hrpB"
                     /locus_tag="ACHKIR_000576"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HFO9988367.1"
                     /translation="MSSLPVAAVLPELLAALDCASQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDEAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVAHFDPRTGLTRLVTQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDTAQMSWLDQPPTVNLQAAKRL
                     LQMLGALEGERLSAQGQKMAVLGSDPRLAAMLVSAKNDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAYADRIACRRGQDG
                     RYQLANGMGAMLDADDALSRYEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLFAAKWLPEYDWPAVDDESLLATLETWLLPHMSGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDKPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLELLSPAQRPLQITRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7444..9975
                     /gene="mrcB"
                     /locus_tag="ACHKIR_000577"
     CDS             7444..9975
                     /gene="mrcB"
                     /locus_tag="ACHKIR_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HFO9988368.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDYDDYDDYEDEEP
                     MPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQLP
                     AAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFDF
                     PDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPRS
                     GFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNLF
                     LSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYYF
                     GRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYDM
                     LSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAAE
                     KAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSIG
                     SLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLVD
                     ALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLNPVPAMLLGALNLTPIEVAQAF
                     QTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGTG
                     RQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAMS
                     IYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSEM
                     QQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10195..12384
                     /gene="fhuA"
                     /locus_tag="ACHKIR_000578"
     CDS             10195..12384
                     /gene="fhuA"
                     /locus_tag="ACHKIR_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HFO9988369.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAAKHSATATKTDTPIEKTPQSISVVTNEEMQMHQFQSVKEA
                     LGYTPGVTVSSRGASNTYDFVIIRGFSSVGLNQNNYLDGLKLQGNFYNDAVIDPYMLE
                     RVELMRGPTSVLYGKSNPGGIISMVSKRPTTEPLKEIQFKMGTDNLFQTGFDFSDALD
                     DNGEFSYRLTGLARSTNEQQKNSESQRYTIAPSFSWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGASNNTYSRNQKMVGYSFEHGFNDTFTVRQNLR
                     FSEMKTSQKSVYGTGIASDGHTLNRGTVVDNERLQNFSVDTQLESKFATGEVEHTLLT
                     GVDFMRMRNDINASFGSAPSIDLYNKYHPEYFAFGNAEPYQMNESKQTGIYVQDQAEW
                     NKWVFTLGGRYDWSKQATTVRENSYTPTEGYIERNDHQFTWRGGVNYLFDNGISPYFS
                     YSQSFEPSAFDLWSNPRVSYKPSKGEQYEAGVKYVPNDMPVVVTGAVYQLTKTNNLTA
                     DPTNPLAQVPAGEIRARGVELEAKAALNANINLTASYTYTDAEYTKDTNLKGKTPEQV
                     PEHMASLWGDYTFNEGPLSGLTLGTGGRFIGSSYGDPANTFKVGSAAVMDAVVKYDLA
                     RFGMAGSSLAVNVNNLLDREYVASCFQTYGCFWGAERQVVATATFRF"
     gene            12435..13232
                     /gene="fhuC"
                     /locus_tag="ACHKIR_000579"
     CDS             12435..13232
                     /gene="fhuC"
                     /locus_tag="ACHKIR_000579"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HFO9988370.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRGGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13232..14122
                     /gene="fhuD"
                     /locus_tag="ACHKIR_000580"
     CDS             13232..14122
                     /gene="fhuD"
                     /locus_tag="ACHKIR_000580"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HFO9988371.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAIIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMIW
                     SAGYGPSPEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRILDNAIGGKA"
     gene            14119..16101
                     /gene="fhuB"
                     /locus_tag="ACHKIR_000581"
     CDS             14119..16101
                     /gene="fhuB"
                     /locus_tag="ACHKIR_000581"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HFO9988372.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVAAERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAINPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16136..17416)
                     /gene="hemL"
                     /locus_tag="ACHKIR_000582"
     CDS             complement(16136..17416)
                     /gene="hemL"
                     /locus_tag="ACHKIR_000582"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HFO9988373.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPHEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17582..17629
                     /gene="yadX"
                     /locus_tag="ACHKIR_000583"
     CDS             17582..17629
                     /gene="yadX"
                     /locus_tag="ACHKIR_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YadX"
                     /protein_id="HFO9988374.1"
                     /translation="MERASKMPSSYLYDQ"
     gene            17641..19062
                     /gene="clcA"
                     /locus_tag="ACHKIR_000584"
     CDS             17641..19062
                     /gene="clcA"
                     /locus_tag="ACHKIR_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HFO9988375.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDVFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19086..19151
                     /gene="yadW"
                     /locus_tag="ACHKIR_000585"
     CDS             19086..19151
                     /gene="yadW"
                     /locus_tag="ACHKIR_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YadW"
                     /protein_id="HFO9988376.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            19144..19488
                     /gene="erpA"
                     /locus_tag="ACHKIR_000586"
     CDS             19144..19488
                     /gene="erpA"
                     /locus_tag="ACHKIR_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HFO9988377.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19535..20158)
                     /gene="yadS"
                     /locus_tag="ACHKIR_000587"
     CDS             complement(19535..20158)
                     /gene="yadS"
                     /locus_tag="ACHKIR_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HFO9988378.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20196..20996)
                     /gene="btuF"
                     /locus_tag="ACHKIR_000588"
     CDS             complement(20196..20996)
                     /gene="btuF"
                     /locus_tag="ACHKIR_000588"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HFO9988379.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLVPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARAPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(20989..21687)
                     /gene="mtnN"
                     /locus_tag="ACHKIR_000589"
     CDS             complement(20989..21687)
                     /gene="mtnN"
                     /locus_tag="ACHKIR_000589"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFO9988380.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21771..23288
                     /gene="dgt"
                     /locus_tag="ACHKIR_000590"
     CDS             21771..23288
                     /gene="dgt"
                     /locus_tag="ACHKIR_000590"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HFO9988381.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLYSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFNKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23418..24842
                     /gene="degP"
                     /locus_tag="ACHKIR_000591"
     CDS             23418..24842
                     /gene="degP"
                     /locus_tag="ACHKIR_000591"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HFO9988382.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            24997..26154
                     /gene="cdaR"
                     /locus_tag="ACHKIR_000592"
     CDS             24997..26154
                     /gene="cdaR"
                     /locus_tag="ACHKIR_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HFO9988383.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEQR"
     gene            complement(26269..26655)
                     /gene="yaeH"
                     /locus_tag="ACHKIR_000593"
     CDS             complement(26269..26655)
                     /gene="yaeH"
                     /locus_tag="ACHKIR_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HFO9988384.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGIGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(26813..27625)
                     /gene="yaeI"
                     /locus_tag="ACHKIR_000594"
     CDS             complement(26813..27625)
                     /gene="yaeI"
                     /locus_tag="ACHKIR_000594"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase YaeI"
                     /protein_id="HFO9988385.1"
                     /translation="MITRRRFLQAAAATLAAGSSLSYMHYWEPGWLELIRHRLAFFKD
                     NAAPFKILFLADLHYSRFVPLSLISDAIALGVAQKPDLILLGGDYVLFDMQLNFSAFS
                     DVLSSLAECAPTFACYGNHDRPVGTEKNRLIGEALKSAGITVLFNEAMLITTQKQQFE
                     LVGTGDLWTGQCKPPPASEANLPRLVLAHNPDSKEVMRNEKWDLMLCGHTHGGQLRIP
                     LVGEPFAPIEDKRYAAGLNAFGERQIYTTRGVGSLYGLRLNCRPEVTILELV"
     gene            complement(27678..28502)
                     /gene="dapD"
                     /locus_tag="ACHKIR_000595"
     CDS             complement(27678..28502)
                     /gene="dapD"
                     /locus_tag="ACHKIR_000595"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HFO9988386.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(28533..31205)
                     /gene="glnD"
                     /locus_tag="ACHKIR_000596"
     CDS             complement(28533..31205)
                     /gene="glnD"
                     /locus_tag="ACHKIR_000596"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HFO9988387.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCAWPRDELTVCGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(31267..32061)
                     /gene="map"
                     /locus_tag="ACHKIR_000597"
     CDS             complement(31267..32061)
                     /gene="map"
                     /locus_tag="ACHKIR_000597"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFO9988388.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            32429..33154
                     /gene="rpsB"
                     /locus_tag="ACHKIR_000598"
     CDS             32429..33154
                     /gene="rpsB"
                     /locus_tag="ACHKIR_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFO9988389.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            33412..34263
                     /gene="tsf"
                     /locus_tag="ACHKIR_000599"
     CDS             33412..34263
                     /gene="tsf"
                     /locus_tag="ACHKIR_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFO9988390.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            34410..35135
                     /gene="pyrH"
                     /locus_tag="ACHKIR_000600"
     CDS             34410..35135
                     /gene="pyrH"
                     /locus_tag="ACHKIR_000600"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFO9988391.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            35427..35984
                     /gene="frr"
                     /locus_tag="ACHKIR_000601"
     CDS             35427..35984
                     /gene="frr"
                     /locus_tag="ACHKIR_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFO9988392.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            36076..37272
                     /gene="ispC"
                     /locus_tag="ACHKIR_000602"
     CDS             36076..37272
                     /gene="ispC"
                     /locus_tag="ACHKIR_000602"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HFO9988393.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            37461..38219
                     /gene="ispU"
                     /locus_tag="ACHKIR_000603"
                     /gene_synonym="uppS"
     CDS             37461..38219
                     /gene="ispU"
                     /locus_tag="ACHKIR_000603"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002094 - polyprenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008834 -
                     di-trans,poly-cis-undecaprenyl-diphosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0043164 - Gram-negative-bacterium-type cell
                     wall biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HFO9988394.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            38232..39089
                     /gene="cdsA"
                     /locus_tag="ACHKIR_000604"
     CDS             38232..39089
                     /gene="cdsA"
                     /locus_tag="ACHKIR_000604"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFO9988395.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            39101..40453
                     /gene="rseP"
                     /locus_tag="ACHKIR_000605"
     CDS             39101..40453
                     /gene="rseP"
                     /locus_tag="ACHKIR_000605"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HFO9988396.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVTSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            40483..42915
                     /gene="bamA"
                     /locus_tag="ACHKIR_000606"
     CDS             40483..42915
                     /gene="bamA"
                     /locus_tag="ACHKIR_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HFO9988397.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            43037..43522
                     /gene="skp"
                     /locus_tag="ACHKIR_000607"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             43037..43522
                     /gene="skp"
                     /locus_tag="ACHKIR_000607"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HFO9988398.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            43526..44551
                     /gene="lpxD"
                     /locus_tag="ACHKIR_000608"
     CDS             43526..44551
                     /gene="lpxD"
                     /locus_tag="ACHKIR_000608"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFO9988399.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            44656..45111
                     /gene="fabZ"
                     /locus_tag="ACHKIR_000609"
     CDS             44656..45111
                     /gene="fabZ"
                     /locus_tag="ACHKIR_000609"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFO9988400.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            45115..45903
                     /gene="lpxA"
                     /locus_tag="ACHKIR_000610"
     CDS             45115..45903
                     /gene="lpxA"
                     /locus_tag="ACHKIR_000610"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HFO9988401.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            45903..47051
                     /gene="lpxB"
                     /locus_tag="ACHKIR_000611"
     CDS             45903..47051
                     /gene="lpxB"
                     /locus_tag="ACHKIR_000611"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HFO9988402.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTTRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNAARDVLGIPHDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHMLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            47048..47644
                     /gene="rnhB"
                     /locus_tag="ACHKIR_000612"
     CDS             47048..47644
                     /gene="rnhB"
                     /locus_tag="ACHKIR_000612"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFO9988403.1"
                     /translation="MIEFVYPHTHLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALCEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEYGATEHHRRSFGPVKRVLGLAS"
     gene            47681..51163
                     /gene="dnaE"
                     /locus_tag="ACHKIR_000613"
     CDS             47681..51163
                     /gene="dnaE"
                     /locus_tag="ACHKIR_000613"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFO9988404.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERVKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            51176..52135
                     /gene="accA"
                     /locus_tag="ACHKIR_000614"
     CDS             51176..52135
                     /gene="accA"
                     /locus_tag="ACHKIR_000614"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFO9988405.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            52234..54036
                     /locus_tag="ACHKIR_000615"
     CDS             52234..54036
                     /locus_tag="ACHKIR_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HFO9988406.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTDEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGERWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDTATRVYSIDLYV"
     gene            55144..55323
                     /locus_tag="ACHKIR_000616"
     CDS             55144..55323
                     /locus_tag="ACHKIR_000616"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9988407.1"
                     /translation="MRKRKGEGVTSGTDAPESGSMVCRNVWRLSFRWWLCLSGMTDTT
                     PLVLWCWLVVLRALV"
     gene            55348..55545
                     /locus_tag="ACHKIR_000617"
     CDS             55348..55545
                     /locus_tag="ACHKIR_000617"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9988408.1"
                     /translation="MLPMWWLPGTAGTTTGVAVIPPLVLCGVLLPWLVVGMVAHVTAV
                     MMCDMVSARYAALCARSGALT"
     gene            complement(55721..56437)
                     /locus_tag="ACHKIR_000618"
     CDS             complement(55721..56437)
                     /locus_tag="ACHKIR_000618"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9988409.1"
                     /translation="MQNDTSDTLLNRILNEPEPVSQLPGRAFLQHVHERTGADTQRQR
                     KEKNRLNSEKLAEKICRWIKETGRGPDAVMYEADLKAFFKCSREELAIAASLIPLYSH
                     QRELHDVRVVAYTAAPPVVLPSDLRPAERFLYHCLKRGYTGLKSEGWPVWIRTKSLYT
                     HFMKSQYFDQLGQSVLSHAVTTSGIGTLAYRREPEGKKNRNTRVARMYPLEQAREAFA
                     TNTLKNPSFDWSVYDPNVDA"
     gene            complement(56713..57021)
                     /locus_tag="ACHKIR_000619"
     CDS             complement(56713..57021)
                     /locus_tag="ACHKIR_000619"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9988410.1"
                     /translation="MPVIYPLRPGDVFGVAGVEMLVVWADTAKRRRCVTVIHALEVIL
                     SNGERRSVTDEQAEEIKGLRPLPPAKSDTDIDNDPLVIRLREIMHRANGGQDWRPDYG
                     "
     gene            complement(57195..57620)
                     /locus_tag="ACHKIR_000620"
     CDS             complement(57195..57620)
                     /locus_tag="ACHKIR_000620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9988411.1"
                     /translation="MSSIKIFNWPDTLVTAAIIEDRPHLFAETGAFCVPMVHVAPSGS
                     HLIYHTLPAKGTYMDFLERGDMVFIFEDVMYLCPMFTDGRLPHAEEEECMAQLVLRHY
                     DARRRGRRPRMSVEILYTNEWCYEESYKMARAESLQEGL"
     gene            complement(57625..60105)
                     /locus_tag="ACHKIR_000621"
     CDS             complement(57625..60105)
                     /locus_tag="ACHKIR_000621"
                     /inference="COORDINATES: protein motif:HMM:NF017826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HFO9988412.1"
                     /translation="MMQPQHVHDSTTVTGAAGSSTDTVTAGHTTGTPARTGAAATPVI
                     ADGIRAELEDFLRDTVAGAASPYLKRKGLESFATKPLVLSGGRTLIPMQDASDVVVAF
                     QTISADGKEKRFGGVAKGAFHTINQQTQPVRIIIAEGLATTLSAHQLQHDAMAVMAGS
                     AGNLEAVARTMRERHPEAEIIIAADNDNTGKGKNTGKVAAEKAALAVGGTVALAPAED
                     GEGRDWNDVLVSEGREKATMLFAGSMYRPTAAEKTTSRKQRRATSDGESGVVIEDTDE
                     GPMMRLRFRDTSLDICQKVDVVGYGSYEGKEYRVLRWMNNGRPATVAVNLGGFASSAA
                     LTTLMDGGLLVRDGRQLRSALANWIIGQKPATRWTLAASAGWTDDNRAYLLPDGQVIG
                     DEIPALFMDKSDVAGGWTARGTADTWRESVARLVDGNSLMMVCVAIPFAGPLLKLAGI
                     QNFGLHLYAPSTTGKTTCATLAASVCGEPSVLKMTWNGTANGIQNKAAARCDGFLWLD
                     ELGEGSPKALASVIYSLFNGSGRVRATKDGSNAPHDSWRLPVLSTGEADTAAFASQAQ
                     ITLAPGQLVRLLNIPVEEMQPENLHEYHTAGAHARALTRACNDNFGAVFREWITWLIR
                     NLPEAGNTIRQRLDEWQAAVQKRFPDSPQVCRVADAFAVLDAALVMARPFTGWNEAAC
                     RDAVVNVFKSWVKNFGTRDKAQSQIISQATDFLMQYGRSQFATLTTDGKANAATDQEA
                     SRMGRLCGYRKPTDGDNGDEWFYVAPAAFRNHIAKGHNYRQAAAALEKAGMLERETGS
                     ESRQSRLRIGGSQQRFYKVKLLDAVEGE"
     gene            complement(60102..60470)
                     /locus_tag="ACHKIR_000622"
     CDS             complement(60102..60470)
                     /locus_tag="ACHKIR_000622"
                     /inference="COORDINATES: protein motif:HMM:NF019878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primase-helicase zinc-binding domain-containing
                     protein"
                     /protein_id="HFO9988413.1"
                     /translation="MTLKTNRAGRYIRAVVEAAEGQWPLILRRLVIDVPPVGQGGPCP
                     ACGGDDRFTFDDKEGRGTFVCRKCPDETDAYYQAGDGLELVRRVASVRFGSPVSTYQA
                     AKLVGNVLGIVEATPTQGAI"
     gene            complement(60708..61049)
                     /locus_tag="ACHKIR_000623"
     CDS             complement(60708..61049)
                     /locus_tag="ACHKIR_000623"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO9988414.1"
                     /translation="MTDKQRTDLLTIPPGAVIFDRLVYEKECKAYTGLCRMTRLRMEK
                     AGTFPVPRRLDDTKTAKNAWLLSELVEWARSRPRADHGPDTGDDEDSDYVTLTSTEEL
                     LFRAKEILDAM"
     gene            complement(61257..62456)
                     /locus_tag="ACHKIR_000624"
     CDS             complement(61257..62456)
                     /locus_tag="ACHKIR_000624"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9988415.1"
                     /translation="MTALSFRQVRDRLNLSSDDDLLSLIARVPQWTDTGAFVDIYSPG
                     MYGHEYRRDGVPLSLDGDSDRYISEEVGPLRLRMWVTREDYRSACHISVPVSPFAYRR
                     VLWGDEETYDYGAEPDSPDTPPHRIRLFAGGMFRIWGAVLTDDLVTFRLAPWRWQQPG
                     VMPLYWQVTRPLPEALTNSMTIRDDGLHILKELLASSTSDAKQLHDANTTAVDQPPAK
                     ANTTPAKTDTAAQNTPYTTDDFDPGVLHAREDAVGRRALAVLVGHVIHQPVNAMDDLP
                     VRLEKMRPGGEFQEVINAWLEKRSDGERQRVEEIAGVSTDSWPDCTRGGETPEHLTRM
                     IWLCLAKLHGVAAVYDADVGDRARKTSNRYGKYGGKHIGNDAEKTAARPSIRRRAEAH
                     LHPIKKD"
     gene            complement(62532..63779)
                     /locus_tag="ACHKIR_000625"
     CDS             complement(62532..63779)
                     /locus_tag="ACHKIR_000625"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO9988416.1"
                     /translation="MGGAINRLKDIELKRRNGVERDKEETLSDGGGLYARCQKGGFIT
                     WYFQYKQKGSEARAVKVYFGRYPDVGLKVARELRQECREWLARGKDPKTEYERRRAAS
                     AEPVKRITVADAITYWLDNYVRNTWKRPGTVISRMNTYIISSIGSRPLDECSAGDWRD
                     CFRKIADKYPYTAGKALGECKQALNYCDAFGYATSNALDKMTVKAVGGVPSGKCARVL
                     DNDELIDIWKAASDGSLNPYYADLMRLLIVFGCRTVEARLSRIDEWDVDKWIWTVPRE
                     HNAKGGIPVRRPVPDAIKPVITRLIQANKKTGLLLGVEKKEKTVGSWGNRAWKMARFR
                     HLKRWSFHDLRRTFSTRHNEDIHTALYVVEQMLGHVMQGVMAVYNRSEYMAEKTEALN
                     KWVEWLDALASGDNVIPLQKTKN"
     gene            64267..64659
                     /locus_tag="ACHKIR_000626"
                     /pseudo
     CDS             64267..64659
                     /locus_tag="ACHKIR_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706131.1"
                     /note="constitutive; catalyzes the formation of cadaverine
                     from lysine; incomplete; partial in the middle of a
                     contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
     gene            64716..65105
                     /gene="yaeR"
                     /locus_tag="ACHKIR_000627"
     CDS             64716..65105
                     /gene="yaeR"
                     /locus_tag="ACHKIR_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFO9988417.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            65170..66468
                     /gene="tilS"
                     /locus_tag="ACHKIR_000628"
     CDS             65170..66468
                     /gene="tilS"
                     /locus_tag="ACHKIR_000628"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFO9988418.1"
                     /translation="MTLTLNRQLLSSRQILVAFSGGLDSTVLLHQLVQWRMENPGVTL
                     RAIHVHHGLSANADAWVRHCENICQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMGEVSEFAGTRLIRPLLARTR
                     GELVQWARQYDLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDIAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGKNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKAPGLLHIIGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(66517..66777)
                     /gene="rof"
                     /locus_tag="ACHKIR_000629"
     CDS             complement(66517..66777)
                     /gene="rof"
                     /locus_tag="ACHKIR_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HFO9988419.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGTTRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(66764..66964)
                     /gene="yaeP"
                     /locus_tag="ACHKIR_000630"
     CDS             complement(66764..66964)
                     /gene="yaeP"
                     /locus_tag="ACHKIR_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HFO9988420.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            67130..67675
                     /gene="yaeQ"
                     /locus_tag="ACHKIR_000631"
     CDS             67130..67675
                     /gene="yaeQ"
                     /locus_tag="ACHKIR_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HFO9988421.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFAYNSRAAQIWWQQNQSKCAQFSNLSIWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            67672..68094
                     /gene="arfB"
                     /locus_tag="ACHKIR_000632"
     CDS             67672..68094
                     /gene="arfB"
                     /locus_tag="ACHKIR_000632"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HFO9988422.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTTEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            68108..68818
                     /gene="nlpE"
                     /locus_tag="ACHKIR_000633"
                     /gene_synonym="cutF"
     CDS             68108..68818
                     /gene="nlpE"
                     /locus_tag="ACHKIR_000633"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414734.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HFO9988423.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEGNPDTGAPTKVLA
                     PDTAGKFYPNQDCSSLGQ"
     gene            complement(68848..69672)
                     /gene="yaeF"
                     /locus_tag="ACHKIR_000634"
     CDS             complement(68848..69672)
                     /gene="yaeF"
                     /locus_tag="ACHKIR_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HFO9988424.1"
                     /translation="MDKPKAHCRLLLPCFLFLSACTVDISQPDSSATAVDVEAKTWVV
                     KFQHQSSFTEQSIKEITAPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIFLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQVCSLNPFSEDFRQQCVSGLAKAQLSSVGEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRTGDISAFKPETQLQYVGHMKPGIYIKAGRFVGLTQ"
     gene            complement(69725..71443)
                     /gene="proS"
                     /locus_tag="ACHKIR_000635"
     CDS             complement(69725..71443)
                     /gene="proS"
                     /locus_tag="ACHKIR_000635"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFO9988425.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVLMPVVQPSELWQESGRWEQYGPELLRIADRGDR
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEIADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(71554..72261)
                     /gene="tsaA"
                     /locus_tag="ACHKIR_000636"
     CDS             complement(71554..72261)
                     /gene="tsaA"
                     /locus_tag="ACHKIR_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HFO9988426.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPNASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(72258..72662)
                     /gene="rcsF"
                     /locus_tag="ACHKIR_000637"
     CDS             complement(72258..72662)
                     /gene="rcsF"
                     /locus_tag="ACHKIR_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HFO9988427.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAESAKPKVP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(72780..73595)
                     /gene="metQ"
                     /locus_tag="ACHKIR_000638"
     CDS             complement(72780..73595)
                     /gene="metQ"
                     /locus_tag="ACHKIR_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HFO9988428.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKESPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(73635..74288)
                     /gene="metI"
                     /locus_tag="ACHKIR_000639"
     CDS             complement(73635..74288)
                     /gene="metI"
                     /locus_tag="ACHKIR_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HFO9988429.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(74281..75312)
                     /gene="metN"
                     /locus_tag="ACHKIR_000640"
     CDS             complement(74281..75312)
                     /gene="metN"
                     /locus_tag="ACHKIR_000640"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HFO9988430.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAITWLQEHH
                     VKVEVLGYV"
     gene            75500..76072
                     /gene="gmhB"
                     /locus_tag="ACHKIR_000641"
     CDS             75500..76072
                     /gene="gmhB"
                     /locus_tag="ACHKIR_000641"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HFO9988431.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAAAANVGTKVLVRTGKP
                     VTPEAENAADWVLNSLADLPQAIKKQQKPA"
BASE COUNT        17707 a        19165 c        20901 g        18517 t
ORIGIN      
        1 taatgatatt gttttggcaa taacttgagg ttgttttcag aatatattta tattcacacg
       61 aataataatt accacttatc taattcatta aagccttttg cctgtagagc ttcaactaat
      121 gataagccat caggaaatag caattccggc gcaatttcaa acagtggcca cagcataaag
      181 ccgcgatttt tcatatcgta gtgcggaacg gtcagacgtt cggtatttat tacttcatta
      241 ccaaacagca tgatgtcgag atccagcgtg cgcggtcccc agcgttcagc tttacggacg
      301 cgaccttgct gcaattcgat acgctgtgtg tgattgagta gctcttcagg ggcaagagtg
      361 gttttcagcg cgacggctgc atttaagtaa tcgggttgat cttgcggccc cagcggtggg
      421 gtgcggtaaa acgaagaaac agcaagaatg cggctttctg gaatatcgcc taatgctttc
      481 agggcagcat tgacctgctc cagcggagag gccagattgc tgcctatggc aatatacgcc
      541 actgtcatgc ggtaccctca cgacgcggtg cgcgtttgcg tggacgacga gtacggcgac
      601 gcggtgacgg ttcttcatcc agttcgttaa gcatcccttt ttggtctggt ggcgcggaaa
      661 cctggaactc accccaccat ttcaccagac gttgcagttc agcgttacgc tcaacttcgg
      721 ctcgtaaggc caacaggtcg tatgccgcac ggaacttagg atgctccagc agtttccagg
      781 cgcgtttacc ctgacgtcgg gacatacgca actgcaactg ccagatatcg cgggttaacg
      841 tcgtcagacg tttcgggatc gccagtgaac gacaggcttc gtccagcacg tcgttcatcg
      901 ccagcgcgaa agcgtcgtga taggtcaggc cgctttcctg ggcgatcttc tgtgccgtct
      961 ccagcagtgg gtaccagaac atggcggcaa acaggaacgc cgggttcacg cgcatatcgt
     1021 tatggatacg cgtatcggta ttcttcagca cctgttcaat gatccgctcc atcgggctgt
     1081 cgccattttc cgtgaagtag cgggtaatgg tcgggaacag cggctggaac aggtgatatt
     1141 cacacagcag cttataggtt tcgtaaccgt agcccgcttg cagcagtttc aaagattctt
     1201 caaacagacg tgccggtgga atatcgttca gcagggtagc caggcgaggg atcggttctg
     1261 cggtttcagg actgatacgc atacccaatt tggcggcaaa acgtaccgcg cgcagcatcc
     1321 gtaccggatc ttcacggtag cgcgtttccg ggttaccaat cagacggata acgccgtcct
     1381 tcagatcctt catgccgcca acgtaatcac ggacggtaaa atctgccacg ctgtaataca
     1441 ggctgttgat agtgaaatcg cggcgctggg cgtcttcttc gatggagccg aaaatgttgt
     1501 cgcgcagcaa catgccgttt tgcccgcgtt gggaggtcgt gcggtcgctg acgttacctt
     1561 cgtgatgtcc acggaaggtc gcaacttcga taatctccgg gccaaacatc acatgagcca
     1621 gacggaaacg gcgacccacc aggcggcagt tacggaacag tttacgtacc tgttcaggcg
     1681 tggcgttagt ggttacgtca aaatctttcg gctttttgcc aagtaacagg tcgcgcacgc
     1741 caccgccaac cagccaggct tcgtatcccg ctttattgag cctgtacatt accttcaggg
     1801 cattttcact gatatctttg cgggaaatag catgctgctc acgcgggatc accgtcacct
     1861 gtggacgggc gactgcctgt tcagcctcgc tttcctcgcg gcttagcacc ttgcggcaaa
     1921 aattagcgac tcgggtaaaa atagtacacc tcggtagtgt caaacatcat tcaggacaaa
     1981 aaaatagcgg ctaatcatag ctcagcatga cgcatttgag aatgttgaat ttacaattgc
     2041 cgactcaggc acggcggtaa gccgccagtt tttgacggct gactgaagga tttgctcgac
     2101 gctgaaatcc tgccagtgtg cttctgcctg ctgacccaga aattgaagtg ccgcgattag
     2161 taccgggcgc ggatcgcctt tcggcaacgc aggcgcatga ttctgcttgg aaagtttagc
     2221 gccttgtgtg ttaagcgcca gcggtaaatg aatgtaatct ggcactttcc agccaaaaag
     2281 ctggtacagc gagatttgcc gcactgtcgg ttcgatcaaa tctgccccgc ggacaatttc
     2341 gctcacgccc tggaagtgat catccaccac caccgccagg ttataggcaa acaaaccatc
     2401 acggcgatga ataataaaat cttcccgcgc cagtttttcg ttggcgtgaa taatgccgcg
     2461 cagctgatca gtaaatagcg tgaccggatg ctgctggcgg atacgtactg cggcgttgtc
     2521 tggtccatga tgcaattccc ggcaatgacc gtcgtaaata ccgccaatgc tttgaatacg
     2581 cgcgcgcgtg caggtgcagt aataacttag tccttgctca tgtaaccagg cgagtgcttc
     2641 gcgataggca tcgtgacgtt gcgattgcca gagaacgtcg ccgtcccagt gcagaccgta
     2701 atgttccagc tggcgcagga tagtctctgc ggcaccggga acttcacgag gcgggtcgat
     2761 atcttcaatg cgtaccagcc agcgaccttg ccgggcgcga gcctgtaaat agctgccaag
     2821 cgcggcaatc agagagccaa aatgaagctc gccggaagga gagggggcaa agcggccaat
     2881 atagtgtgtg tctgtcatct cgttgaacaa aaaataaggc gggagcattt cccgcctgtg
     2941 gtaaacgtga tggaacggct gtatttagcc agccatttgt ttttcgcgaa tttcagccag
     3001 cgttttgcag tcgatgcaca gatcggctgt cgggcgcgct tccagacggc gaataccaat
     3061 ttcaacaccg caggattcgc agtagccgaa atcttcgtct tccacttttt tcagcgtctt
     3121 ctcgatcttt ttgatcagct tacgctcgcg atcgcggtta cgcagttcga ggctgaactc
     3181 ttcttcctgg gctgcacggt ctaccgggtc cgggaagttg gctgcttcat cctgcatatg
     3241 tgtaacggtg cgatcgactt catccctgag ttgattacgc catgcttcca gaatacgacg
     3301 gaagtgcgcc agctgggctt cattcatata ctcttcgccc ggcttctcct gatatggttc
     3361 caccccagcg atggcgagaa tactcaggga cgatgtttta cggttttgcc cttcttgcat
     3421 gttgcttctc cttaacacgc actatcgatc cccatgttcg ggggaaaaat gaggccgcta
     3481 taaatagcag atgcttttcc ggatagcaat tatctaaacg taacacttga caactgtgtg
     3541 aggaaaagcg tatttgcgca cgcgaccaga atgtaaatta accagttaca tactttacta
     3601 caatgtaacc ggcagtgatt ttttaagagc catgccttca gcagaaagtt ccgctttgta
     3661 agccagaatt tctacccccc tctgttgagc ttctgacaat agttgcgcgt atttctcatc
     3721 gatgtggcgc gcgggtgaaa accgtgtaat ggctgaatgc agcacggcga aaaagataac
     3781 cgcacgctgg ccttcagccg ctacgctcat caactcccga aggtgtttct gacctcgttc
     3841 agtgaccgca tcgggaaaat atccctgttc gttctccgct aacgtaaccg atttcacttc
     3901 aatatagcag tctggacgcg aatccgcctg caacataaag tcaatacggc tgcgttcggc
     3961 gccgtatttt acttcgcttt tcagcgagct atagcctgac agctctgaaa ttgattcatt
     4021 aaggatagcc tctttcgtca acctgttagc ccaaagcgtg ttgacgcaaa taaatgcgcc
     4081 gctctggctt tgagtcaatt cccaggtgtg tgggtatttc cgtttggtgt tgtctgaagt
     4141 cgaataccag acggtatcgc caggcgttgc acaactggtc atcgcacccg tattcgggca
     4201 gtgtagcgtt aattcgcgac catcgggtgt gatcacatcg gctaaaaaac gtttgtaacg
     4261 ctgaattagc gtcgcgcgct gtagaggggg agaaaattcc attacgacaa tccttgttat
     4321 tgcgttagcg cccagcgttt tagcggcgtg tagcgtgtgc gtccacgggc aaacgaggag
     4381 gcgtaaaggg tgaactccgt caccgtatac gaccagttaa aacctggcgg cgggattgtc
     4441 accgcctcgc tggcgtcgcg caataaggta atatgtggat gaaacggacg attgctttga
     4501 aaacaaccgc tgcgggcagc ctgtgaacgg agcatattcg ccagctggat taagccgcgt
     4561 ggtggctgac gcatccctaa ccacaccaca cgcgaacgca gccattgacc ggcgtcatca
     4621 agcgtgagtg tgaaacccgg ttgacgaatc cgcccggcta aaagagaaag cgccttctct
     4681 ttctcggcgc tcacttcgcc taaaaatgcc agagtcagat gcaaattatc ggcggcgacc
     4741 ggacgtcccg cgtcaggtgg gaagtgtgtg gcgcgccagt ggataatctg ttcgcggatt
     4801 tctgcaggta agtcgatagc aaagaacagc cgttgcggtt cagacatgtg aggcactcgg
     4861 ttatgaatta ccgcgatgct acaatgtggc gcgaagaatg ttaaccctct ggagcgtttt
     4921 gtgtcgtcgt tgcccgttgc tgccgtctta cctgagttac ttgctgctct cgattgtgca
     4981 tcgcaggtgt tattaagtgc gccgaccggg gccgggaaat caacctggct gccgctgcaa
     5041 ctgctggcgc atcccggcat taacgggaaa attatcctgc tggagccgcg tcgtctggcg
     5101 gcgcgtaacg ttgcgcaacg actggcggag ctgcttaacg aaaagccagg cgataccgtt
     5161 ggctaccgga tgcgtgcgca aaactgcgtc gggccgaata cccgcctgga agtggttacc
     5221 gaaggcgtgc tgacgcggat gatccagcgt gacccggaac tgagcggtgt tggattggtg
     5281 atccttgatg aatttcatga gcgcagcttg caggcggatt tggcgttggc gctgttactc
     5341 gatgtgcaac aaggtctgcg tgatgacctt aaactgctga ttatgtcggc tacgctggac
     5401 aacgaccgct tgcagcaaat gctgccagaa gcgcccgtcg tcatctcaga agggcgctcg
     5461 tttccggttg aacgccgtta tttgccgctg cccgctcatc agcgttttga cgaagccgtc
     5521 gcggttgcca ccgccgaaat gctgcgtcag gaaagtggat cattactgtt atttttacct
     5581 ggcgtcggag aaattcagcg tgtgcaggaa caactggctt cacgcatcgg cagtgatgtg
     5641 ttgctctgcc cgctgtatgg cgcgttgtcg ctgaacgatc agcgaaaagc gatcctcccg
     5701 gcaccgcaag ggatgcgtaa agtggtgctg gcgaccaata ttgctgaaac cagtttaacc
     5761 atcgaaggta ttcgtctggt ggtggattgt gcccaggagc gtgtggcgca ttttgatccg
     5821 cgtacagggc ttacgcggct ggttactcaa cgcgttagtc aggcgtcgat gacgcaacgt
     5881 gccgggcgtg ccgggcgtct ggagccgggt atctgcctgc atttaatcgc caaagaacaa
     5941 gcagaacgcg ctgccgcgca aagtgaaccg gagatcttac aaagcgatct ttccggtttg
     6001 ctgatggaat tactgcaatg gggatgcagc gatacggcgc agatgagctg gctggatcaa
     6061 ccgccaacgg tgaatctaca ggccgcgaaa cggctgttac aaatgctggg ggcgctggag
     6121 ggtgaacggc ttagcgcaca agggcaaaaa atggcagtgc tgggtagcga tccgcgttta
     6181 gcggcaatgc tggttagcgc gaagaacgac gacgaagctg ctaccgcggc aaaaattgcc
     6241 gccattctcg aagagccgcc acggatgggc aatagtgacc tgggcgtggc gttttcgcgc
     6301 aatcaacccg cctggcagca acgtagtcag caactgttaa aacgcttaaa cgtacgcggc
     6361 ggtgaggcag acagttcgct tatcgcgccg ctacttgccg gggcgtatgc cgatcgcatt
     6421 gcttgtcgcc gtgggcaaga tggacgctat caactggcaa acggcatggg ggcgatgctc
     6481 gatgccgacg atgcgctaag ccgctacgaa tggttgatcg caccgttatt attgcagggc
     6541 agcgcctcgc cggatgcgcg gattttactg gcgctaccgg tcgatatcga tgaattagta
     6601 caacgctgtc cgcagctggt acagcagtcg gacaccgtgg aatgggatga cgcgcaaggc
     6661 acgctgaaag cctggcgtcg gctgcaaatc ggtcagttga cggtgaaagt gcagccactg
     6721 gcgaaaccct cggaagacga gttgcatcag gcgatgctta acggcatccg tgataaaggt
     6781 ttaagcgtgc tcaactggac ggcggaagcg gaacagctac gcttgcgttt gttattcgcc
     6841 gcaaagtggt tgccggaata tgactggcca gcggttgatg atgagagttt gttggcgacg
     6901 ctggaaacgt ggctactgcc gcatatgagc ggcgtgcatt cactacgcgg cctgaaatca
     6961 ctcgacattt atcaggcgct acgcggatta cttgattggg gaatgcagca acgtctggat
     7021 agtgaattgc ctgcgcatta cactgtgccg acgggaagcc ggatcgccat tcgttatcat
     7081 gaagataaac cgcccgcgct ggcggtgaga atgcaggaga tgtttggtga ggccaccaat
     7141 ccgacgatcg cccaggggcg cgtgccgctg gtgctggagt tgctttcccc tgcccaaagg
     7201 ccattacaaa tcacacgaga tttgagcgcc ttctggaaag gagcgtaccg tgaggtgcaa
     7261 aaagagatga aagggcgtta tcccaaacat gtctggccgg acgacccggc aaatactgca
     7321 ccgacgcgac ggacgaaaaa gtattcgtga agaccaaagc aaatttgaga gatatcttct
     7381 tctgtcttgc aacagaagaa cagaaaatcg ggcttttgcg cctgaatatt gcggagaaaa
     7441 agcatggccg ggaatgaccg cgagccaatt ggacgcaaag ggaaaccgac gcgtccggtc
     7501 aaacaaaagg taagccgtcg tcgttacgaa gatgacgatt acgacgatta tgatgactat
     7561 gaggatgaag aaccgatgcc gcgcaaaggt aagggcaaag gcaaagggcg taagcctcgt
     7621 ggcaaacgcg gctggctatg gctgctgcta aaactggcta tcgtttttgc cgtgctgatc
     7681 gccatttacg gcgtttatct cgatcaaaaa attcgcagcc gtattgatgg caaggtctgg
     7741 caattgcccg cggcagttta tggccgaatg gtcaaccttg agccagacat gaccatcagc
     7801 aagaacgaaa tggtgaagct gctggaggcg acccagtatc gtcaggtgtc gaaaatgacc
     7861 cgtcctggcg aatttaccgt gcaggccaac agcattgaga tgatccgccg tccgtttgat
     7921 ttcccggaca gtaaagaagg gcaggtgcgt gcgcgtctga cctttgatgg cgatcatctg
     7981 gcgacgatcg tcaatatgga gaacaaccgt cagtttggtt tcttccgtct tgatccgcgc
     8041 ctgatcacca tgatctcctc gccaaacggt gagcagcgtt tgtttgtgcc gcgcagtggt
     8101 ttcccggatt tgctggtgga tactttgctg gcgacagaag accgccattt ctacgagcat
     8161 gatgggatca gtctctactc catcggacgt gcggtgctgg caaacctgac cgccggacgc
     8221 acggtgcagg gtgcgagtac gctgacgcaa cagctggtga aaaacctgtt cctctccagc
     8281 gagcgctctt actggcgtaa agcgaacgaa gcttacatgg cgctgatcat ggacgcgcgt
     8341 tacagcaaag accgtattct tgagctgtat atgaacgagg tgtatctcgg tcagagcggc
     8401 gacaacgaaa tccgcggctt cccgctggcg agcttgtatt actttggtcg cccagtagaa
     8461 gaattgagcc tcgaccagca ggcgctgttg gtgggtatgg tgaaaggggc gtccatctac
     8521 aatccgtggc gtaacccgaa actggcgttg gagcgacgta atctggtact gcgtctgctg
     8581 caacagcaac agattattga tcaagaactc tatgacatgt tgagtgcacg tccgctgggg
     8641 gttcagccgc gcggtggggt gatctctcca cagccagcct ttatgcaact ggtgcgtcag
     8701 gagctgcagg caaaactggg cgataaggta aaagatctct ccggcgtgaa gatcttcact
     8761 acctttgact cggtggccca ggacgcggca gaaaaagccg ccgtggaagg cattccggca
     8821 ctgaagaaac agcgtaagtt gagcgatctt gaaaccgcga ttgtggtcgt cgaccgcttt
     8881 agtggtgaag ttcgtgcgat ggttggaggt tctgagccgc agtttgcggg ctacaaccgt
     8941 gcgatgcagg cgcgtcgttc gattggttcc cttgcaaaac cagcgactta tctgacggcc
     9001 ttaagccagc cgaaaatcta tcgcctgaac acgtggattg cggatgcgcc aatcgcgcta
     9061 cgtcagccga atggccaggt ctggtcaccg cagaacgatg accgtcgtta tagcgaaagc
     9121 ggcagagtga tgctggtaga tgcgttgacc cgttcgatga acgtgccgac ggtgaatctg
     9181 ggcatggcgt tggggctgcc tgcggttacg gagacctgga ttaaactggg tgtgccgaaa
     9241 gatcaactga atccagttcc agcaatgttg ttgggggcgt tgaacttaac gccaatcgaa
     9301 gtggcgcagg cgttccagac catcgccagc ggtggtaacc gtgctccgct ttctgcactg
     9361 cgttcggtaa tcgcggaaga tggcaaggtg ctgtatcaga gcttcccgca ggcggaacgc
     9421 gctgttccgg cgcaggcggc gtatctgaca ctatggacca tgcagcaggt ggtacaacgt
     9481 ggtacggggc gtcagcttgg ggcgaaatac ccgaacctgc atctggcagg gaaaacaggg
     9541 actaccaaca ataacgtaga tacctggttt gcgggcattg acggcagtac ggtgaccatc
     9601 acctgggtag gccgtgataa caaccaaccg accaaattgt atggagccag cggggcaatg
     9661 tcgatttatc agcgttatct ggctaaccag acgccaacac cgctgaatct tgttccgcca
     9721 gaagatattg cagatatggg cgtggactat gacggcaact ttgtttgcag cggtggcatg
     9781 cgtgtcttgc cggtctggac cagcgatccg caatcgctgt gccagcagag tgagatgcag
     9841 cagcagccgt caggcaatcc gtttgatcag tcttctcagc cgcagcaaca gccgcaacag
     9901 caacctgctc agcaagagca gaaagacagc gacggtgtag ccggttggat caaggatatg
     9961 tttggtagta attaacgtct aagcgtgaca taccggatgg cgagtagcca tccggtaaaa
    10021 taacatccca cccaaagtat taaccctttc ttttcatctg gttgtttatt aacccttcag
    10081 gaaagctcag attgcgtacc gcttgcgaac ccgccagcgt ttcgaatatt atcttatctt
    10141 tataataatc attctcgttt acgttatcat tcactttata tcagagatat accaatggcg
    10201 cgttccaaaa ctgctcagcc aaaacactca ctgcgtaaaa tcgcagttgt agtagccaca
    10261 gcggttagcg gcatgtctgt ttatgcacag gcagcggttg aaccgaaaga agacactatc
    10321 accgttaccg ctgcacctgc gccgcaagaa agcgcatggg gaccggctgc aactattgcg
    10381 gcgaagcact ctgctactgc gactaaaacg gatacaccaa ttgaaaaaac gccgcagtct
    10441 atttcggttg tgacaaatga agagatgcag atgcatcaat ttcagtctgt aaaagaagca
    10501 ttaggttata cacctggcgt tacggttagc agtcgtggtg cttctaatac atatgatttt
    10561 gttattattc gtggtttctc atctgttggt ctgaatcaaa ataattacct tgatggacta
    10621 aaacttcagg gcaactttta taacgatgct gtgattgatc cttacatgct cgagagggtt
    10681 gaactgatgc gcgggccgac gtctgttctc tatgggaaaa gtaatcctgg tgggattatt
    10741 tctatggtga gtaagcgtcc gacaactgaa cccctgaaag aaattcagtt taaaatgggg
    10801 acggataatc tgtttcagac cggattcgat ttcagtgatg cgctggatga taacggtgag
    10861 ttctcctacc gtttgactgg cctcgcacgc tcaacaaacg aacagcagaa aaactctgaa
    10921 tctcagcgtt ataccattgc tccatcattc tcatggcgtc cagacgacaa aaccaatttt
    10981 actttcctgt cctattttca gaacgaacca gaaacgggtt attacggttg gttgccgaaa
    11041 gaggggaccg ttgagccatt gcctaatggt aagcgtctac cgactgactt caatgaaggt
    11101 gcatcgaata atacatactc ccgtaaccag aaaatggtgg gatatagttt tgaacatggt
    11161 tttaatgaca ccttcaccgt gcgtcagaat ctgcgtttca gtgaaatgaa aacctcacag
    11221 aaaagtgttt atggcacagg gattgccagc gatggtcata ccctaaaccg cgggacagtg
    11281 gtggataatg agcgtctgca aaactttagc gttgataccc aacttgaaag taaatttgct
    11341 acaggtgaag tcgagcatac tttgctgaca ggggtagact tcatgcgtat gcgcaatgat
    11401 atcaatgcca gctttggatc tgcaccatcc atcgatcttt ataacaaata tcatcctgaa
    11461 tactttgcat ttggtaacgc agagccatac caaatgaatg aaagcaaaca aacaggtatt
    11521 tatgttcagg atcaggcgga atggaataaa tgggtattca ctctgggggg acgctacgat
    11581 tggtctaagc aagcgaccac tgttcgtgaa aactcttata cgccgactga aggttatatt
    11641 gagcgtaatg atcatcagtt cacctggcgc ggtggtgtaa actacttatt cgataatggt
    11701 atttcacctt actttagtta tagccagtcc tttgaaccga gtgctttcga tctgtggagc
    11761 aacccgcgcg tttcctataa gccatcgaaa ggtgaacagt atgaagctgg cgtaaaatat
    11821 gttccgaatg atatgccggt cgttgttacg ggcgcagtct atcaattgac gaaaacaaat
    11881 aacctgacag cagacccaac aaacccgtta gcgcaagtcc cagcaggtga gattcgcgct
    11941 cgtggtgtgg aacttgaagc aaaagcggcg ttaaatgcca atattaactt gacggcttct
    12001 tatacctaca cagatgcgga atacaccaaa gacaccaatc tcaaaggtaa aactccagaa
    12061 caagtaccgg agcatatggc atctctctgg ggggattata ccttcaatga agggccgctt
    12121 tctggtttaa cattgggaac aggtggtcgt tttattggtt ccagctatgg tgatccggcg
    12181 aacactttta aagtgggtag cgcagctgta atggatgctg ttgtaaaata tgatctggca
    12241 cgctttggta tggcgggatc cagccttgct gtgaacgtca acaatttgct cgatcgtgag
    12301 tatgttgcca gttgcttcca gacctatggc tgcttctggg gcgcagaacg tcaggtcgtt
    12361 gcaaccgcaa ccttccgttt ctaatttttt ctttggggca cggatttccg tgcccgcttc
    12421 acaagttggc tgttatgcag gaatacacga atcattccga taccactttt gcactgcgta
    12481 atatctcctt tcgtgtgcca gggcgcacgc ttttgcatcc gctgtcgtta acctttcctg
    12541 ccgggaaagt gactggtttg attggtcaca acggttctgg taaatccact ctgctcaaaa
    12601 tgcttggccg tcatcagcca ccgtcggaag gcgaaattct tcttgatgcc cagccgctgg
    12661 aaagctggag cagcaaagcg ttcgcccgca aagtggctta tttgccgcag cagcttcctc
    12721 cggcagaagg gatgaccgtg cgtgaactgg tggcgattgg tcgttacccg tggcatggcg
    12781 cactggggcg ctttggtgcg gcagatcgcg aaaaggtcga agaggcgatt tcgctggttg
    12841 gcttaaaacc gctggcgcat cggctggtcg atagtctctc tggcggcgaa cgtcagcggg
    12901 cgtggatcgc tatgctggtg gcgcaggata gccgttgtct gttgctcgac gaaccgacct
    12961 cggcgctgga tatcgcccac caggttgatg tgctggcgct ggtgcatcgt ttaagtcagg
    13021 aacgtggtct gacggtcatt gccgtgctgc acgatattaa tatggctgcg cgctactgtg
    13081 attatctggt cgccctgcgc ggtggtgaaa tgattgctca gggaacgcct gcggaaatta
    13141 tgcgcggcga aaccctcgaa atgatttatg gcatcccgat gggtattctg ccgcatccgg
    13201 cgggtgctgc acctgtgagt tttgtttatt gatgagcggc ttacctctta tttcgcgccg
    13261 ccgactgtta acggcgatgg cgctttctcc gttgttatgg cagatgaata ccgcccacgc
    13321 ggcgattatt gatcccaatc gtattgtggc gctggagtgg ttgccggtgg aattactgct
    13381 ggcgctcggc atcgtgcctt acggcgtggc ggacaccatc aactatcgcc tgtgggtcag
    13441 cgaaccgcca ttgccggact cagtgattga cgttggtttg cgtacagaac ccaaccttga
    13501 actgctgacc gaaatgaaac cgtcgtttat gatctggtcg gcaggatatg gcccttcacc
    13561 agaaatgctg gcacgtattg cgccggggcg cggatttaac ttcagcgacg gcaaacagcc
    13621 gctggcgatg gcgcgtaaat cactgacgga aatggcagat ttacttaacc tgcaaagcgc
    13681 agcagaaacg catttagcgc aatatgaaga ctttatccgc agcatgaaac cccgctttgt
    13741 gaagcgtggt gcacgcccgt tattgctgac gacgcttatc gatccgcgcc atatgttggt
    13801 cttcggtcca aacagcttgt tccaggaaat tcttgatgag tacggcatcc caaatgcctg
    13861 gcaaggggaa accaacttct ggggcagtac cgccgtcagt atcgatcgtc tggcggcgta
    13921 taaagacgtt gatgtgctct gttttgatca cgacaacagc aaagacatgg atgcgctaat
    13981 ggcaaccccg ctgtggcagg ccatgccgtt tgtccgtgca ggacgttttc agcgcgttcc
    14041 ggcggtctgg ttctatggcg cgacgctttc ggcaatgcac tttgtgcgta ttctggataa
    14101 cgccatcgga ggtaaagcgt gagtaaacga attgcgcttt ttccggcgtt attgctggcg
    14161 ctgctggtga ttgtcgctac ggcgctcacc tggatgaact tctcgcaggc gttgccgcgc
    14221 agccagtggg cgcaggccgc ctggtcgccg gatattgacg tcatcgagca gatgattttt
    14281 cactacagct tgttgccgcg tctggcgatt tcgctgctgg tgggcgcggg cctggggctg
    14341 gtgggcgtgc tgtttcagca agtgctgcgt aacccgctgg cggaaccgac gacgttgggc
    14401 gttgcaacag gcgcgcaact ggggattacc gtcactacgc tctgggcgat cccaggtgct
    14461 atggcgagcc agtttgctgc gctggcaggg gcttgcgttg ttggcttaat tgtctttggc
    14521 gtcgcgtggg ggaaacggct gtcgccggta acgctgatcc tcgcggggct ggtagtgagc
    14581 ctttattgtg gggcaatcaa tcagttactg gttatcttcc atcacgacca gctacaaagc
    14641 atgttcctgt ggagcaccgg aacgctgacg caaaccgact ggggcggcgt tgagcgttta
    14701 tggccgcagc tgctgggcgg cgtgatgctg acgttattgc tacttcgccc gttaaccctg
    14761 atggggcttg atgatggcgt ggcgcgcaat ctcggtctgg ccttgtcgct ggcgcgtctg
    14821 gcggcgctgt cgctggcgat agtcatcagt gcgctgctgg tgaatgcagt ggggattatc
    14881 ggctttatcg ggttgttcgc gccgctgctg gcaaaaatgc tgggggcgcg gcgtctgctg
    14941 ccacggttaa tgctggcgtc gctgattggt gcgttaattc tgtggctttc tgatcaaatc
    15001 atcctctggc tgactcgcgt gtggatggaa gtttccaccg gttcggtcac tgcgttgatc
    15061 ggtgcgccgc tgctactgtg gctgctgccg cgtttacgca gcattagcgc gccggatatg
    15121 aaggtcaacg atcgtgtcgc ggctgaacgc caacatgtgc tggcgtttgc cctcgcgggc
    15181 ggcgtgctgc tgttgatggc tgtggtagtg gcgctgtcgt ttggtcgtga tgcgcacggc
    15241 tggacgtggg cgagcggggc gttgctcgag gatttaatgc cctggcgctg gccgcgaatt
    15301 atggcggcgc tgtttgcggg cgtcatgctg gcggtggcgg gctgtattat tcagcggctg
    15361 accggaaacc cgatggcaag cccggaagtg ctggggatta gctccggcgc ggcgtttggc
    15421 gtggtgttga tgctgtttct ggtgccgggt aatgcctttg gctggctgtt acctgcaggc
    15481 agtctcggcg cggcggtgac gctgttgatc attatgatcg ccgccggacg cggtggattt
    15541 tccccacacc gcatgttact ggcggggatg gcgttaagta ccgcgttcac catgcttttg
    15601 atgatgttgc aggcaagtgg tgacccgcga atggcgcaag tgctgacctg gatctccggt
    15661 tcgacctaca acgcgaccga tgcgcaggtc tggcgcacgg gaattgtgat ggtaattttg
    15721 ctggcgatta acccgctgtg ccgccgctgg ctgaccattt taccgctggg cggtgatacc
    15781 gcacgagccg taggaatggc gctgacgccg acgcgaattg cgctgctgct attagcggct
    15841 tgcctgacgg cgaccgcgac gatgactatt ggaccgttga gttttgttgg tttaatggca
    15901 ccgcatattg cgcggatgat gggttttcga cggacgatgc cacacatcgt aatttcggcg
    15961 ctggtgggtg gtttactgct ggtgttcgct gactggtgtg ggcggatggt attgtttcca
    16021 ttccagatcc cggcggggct gctgtcaacc tttatcggcg cgccatattt tatctatttg
    16081 ttgagaaagc agagccgtta atagaatgcc tcgcccggtg ggcgaggcaa aatcatcaca
    16141 acttcgcaaa cacccgacgt gcagcatcga tggtgttatt gatatcttcc atgctgtgcg
    16201 ccacggacat aaagcccgct tcaaacgctg acggtgccag ataaacacct tcgtccagca
    16261 tcatatggaa gaaacgctta aagcgttcca cgtcacaggc catcacatcc tgatagcacg
    16321 tcacggactc ggcgtcggta aagaaaatac cgaacatgcc gccaacgtgg tttaccacca
    16381 gcggaattcc ggcttcttct gccgcttcca gcagaccttc tgccagacgt gttgtcagct
    16441 catccagcgt ctcgtgaacg cccggctgtg cgacttcatt cagacaggcg aaaccagctg
    16501 ccatcgcaat cgggttaccg gaaagcgtac ccgcctgata gaccggacca gtcggggcca
    16561 gcgcatccat tacatcacga cgaccaccga atgcgcctac cggcattcca ccgccgatga
    16621 ttttgccgag gcaggttaaa tctggcacta cgccgtaata atcctgtgcg ccagctagcg
    16681 ctacgcggaa accggtcatc acttcatcga tgatcagcaa cgcgccaaat tcgtcacaca
    16741 gcgcacgcag acctggcagg aactctggca gcggtggaac gcagttcata ttgcctgcca
    16801 ccggctcgac gataatacag gcaatctcat gcgggtactg ttcgaacgcg gcgcgtacag
    16861 aggccagatc gttataagta caggttaagg tatgtttggc gaaatctgcc ggaacacccg
    16921 gcgagtttgg ctggcctaac gtgagtgcgc cagaaccggc tttcaccagc aggcagtcag
    16981 cgtgaccgtg gtaacaacct tcaaatttaa tgattttgtc gcgaccggta aaaccacggg
    17041 ccaggcggat tgcgctcatg gtcgcttcag tgccggagtt caccatgcgc accatatcca
    17101 tggtcgggac cagttcggtc accagttgcg ccattttcac ttccatttcg gtcggtgcac
    17161 caaagcttaa accacgctcg gcggcttcaa tcacggcatt gcggatcgcc ggatggttat
    17221 ggcccagcac catcggcccc caggaaccga cataatcgat ataggctttg ccatcaacat
    17281 cgtacagata agcgccgtcc gctttttcga taaacagtgg agtgccgccc acgccagtaa
    17341 aggcgcgaac aggggagttc acaccgccag ggatcagctc gcgcgctgcg ctgtaaagat
    17401 tttcagactt actcatggag ggttcctgat tcgtagaaaa agtgaatggc tgctattcta
    17461 tgttattcat aacaagttaa atactcgtca aacatcaggc tgcttgtact ggtgcaatcc
    17521 tgatttctta gagtataaaa gttttgtgca tttgaaacat tacgctttgc aaaggatttt
    17581 catggaacgt gcgagtaaaa tgccgtcatc ttatttgtat gaccaataag tgatcattgg
    17641 atgaaaactg atactccctc tttagaaaca ccgcaggccg cgcgcctgcg acgcagacaa
    17701 ctgattcgcc aacttcttga gcgcgataaa accccgttag ccattttgtt tatggcggca
    17761 gtcgtcggca cgcttgtcgg gctggctgcg gttgcttttg acaaaggtgt cgcctggttg
    17821 cagaaccaac gtatgggggc actggtacat actgctgata attatccgct actgttaacc
    17881 gtcgcttttc tctgttcggc ggtgctggcg atgttcggct actttttggt gcgcaaatac
    17941 gcgccggaag caggtggttc ggggatcccg gaaattgaag gggcgctgga agatcaacgt
    18001 cccgttcgct ggtggcgtgt gttgccggtg aaattctttg gcgggctggg aacactcggc
    18061 ggaggcatgg tgctgggccg cgaagggcca accgtacaga tcggcggcaa tatcggtcgc
    18121 atggtgcttg atgttttccg cctgaaaggt gatgaagcgc gccatacgct gctggcaacc
    18181 ggtgctgcgg cggggctggc tgcggccttt aacgccccgc tggcgggtat tttgtttatt
    18241 atcgaagaga tgcgtccgca gtttcgctat acgttaattt cgattaaagc ggtatttatt
    18301 ggcgtcatta tgtcgaccat tatgtaccgg atttttaatc atgaagttgc gttgattgac
    18361 gtcggtaaac tttctgacgc gccgcttaat acgctgtggc tttatctgat cctcggtatt
    18421 atttttggca ttttcggccc tatttttaat aaatgggtgc tggggatgca ggatttgctg
    18481 caccgtgtgc acggcggcaa tattaccaaa tgggtactga tgggcggtgc aattggcggt
    18541 ctgtgtggat tgctggggtt tgtggcacca gcaacgtcgg gcggcggttt taacctgatt
    18601 cctatcgcta ccgcggggaa tttcagcatg ggaatgctgg tgtttatctt cgtcgcgcgg
    18661 gtcattacca ccttactctg cttctcttcc ggcgcgccgg gcggtatttt tgccccgatg
    18721 ctggcgctgg gtactgtgct gggaaccgct ttcggaatgg ttgccgttga gctgtttccg
    18781 caatatcacc ttgaggcggg gacatttgct attgccggaa tgggggcatt actggcggca
    18841 tctattcgcg cgccgttaac ggggatcatt ctggttctgg aaatgaccga taactaccag
    18901 ctcattttgc caatgattat taccggtctt ggcgcaacac tattagcgca atttaccggc
    18961 gggaaaccgc tatactcggc gattcttgcg cgcacgctgg cgaaacagga agctgagcaa
    19021 ctggcgcgaa gcaaggccgc atcagccagc gagaatactt gaacgaaata ccagggtatt
    19081 agataatggc gattattatt gggttagaat ttgcccaatt gccgatgtcg tttggagcaa
    19141 aatatgagtg atgacgtagc actgccgctg gagtttaccg acgcagcagc caacaaagtt
    19201 aaaagcctga tcgctgacga agataacccg aatctgaaat tacgcgtgta tattaccggt
    19261 ggcggttgca gcggcttcca gtatggtttc acctttgatg atcaggtgaa cgaaggcgat
    19321 atgaccatcg aaaaacaggg cgttggcctg gtggttgatc cgatgagcct gcaatatctg
    19381 gtcggcggtt ccgttgacta taccgaaggt ctggaaggtt ctcgtttcat cgtgaccaac
    19441 ccgaacgcga aaagcacctg cggttgcggt tcttccttta gcatctaatc tgttgttcat
    19501 gtgccggata aactatccgg cacatcttct ctcttcaacg cccattctca tccagcgcaa
    19561 acgtcggtag cttaagatgc caacgaatag ccgccagccg aatcaatagc gtcacgacca
    19621 tgcccatcat actggctgtt tccagtggta cggaaaatgt gtaataagcc gtagcgtgga
    19681 caataccgcc gataatacag gctgttgcgt agatttctgt acgtaaaatc atggggatct
    19741 cgcgggcaag tacatcacga atgatcccgc cgccaacgcc agtaatgacg cccatacaaa
    19801 ccgcgattaa cggaccggct tccgcattaa aggctttatt cacgccaatg ccgacaaaca
    19861 ctgccagacc aacggcgtcc agcaccggca acatccattt tggtaagcgt cttggctggc
    19921 gcaccagcac gatggtcagc atgctggtga ccattgcaac gaccagatcg gtgggatctt
    19981 tcacccaaaa taccgggccg tgatccagcg ccatgtcgcg aattgttccg ccgcctactg
    20041 cggtaaccac gcccaatacc agaacaccaa aagggtccat acgcaatttt ccggctaaca
    20101 aaacgccaga gatagcaaat accgctgtgc cgactatatc cagccaatag acgagcattg
    20161 ttcaatcccc actgagcacc tgaaaaggtc aggcattaat ctacctgtga aagcgcatta
    20221 cagagctgtt gtgcagcgag gataatacgt gggcttgcac gttcaaacca gtcactcgtg
    20281 agaggaataa cgggaatttt gagctgttca ccccagtatt gtttgatttt aggaatttgg
    20341 tccggtccgc ctgtaatgac aatcgcctgt ggcgcgcgtg ctaacacctg ttcgcggcta
    20401 acttgcggcc agggaacccg gctgtcttta aagatgtttt ctccgccaca aacttcgagt
    20461 acctggtttt gaatcgactc ttttccacta gtaaatggcg gattaatgcc gaattgcaga
    20521 aaaacacgtt ttttaggttt atcagcatat tgcgctttca attgcgcgta ctggtccagc
    20581 agggattgcg cggcttgttc ggccttatcc ggttgcggac tccaggggac cagttgacgt
    20641 aacgcattgg caatttgttc aatgcttgtc gcatcgaccc acatcacttt tattcccaga
    20701 gaagccagct ggtcaacctg ccgctcggca ttacctccac gccaggcaat caccagatcg
    20761 ggtttcagcg ccacaatgcg ttccagattc atcccctgcc aggtggaaac ctgctcaatt
    20821 ttttgcgctt gtggaggata gtcggaatag ctgctgaccc caaccggcgt gatcccggcg
    20881 gcaaaggcaa gttcagtgtt ggcgggagaa agcgtgatga cgcgcggcgc ggcgttgagc
    20941 cacagcggcg cgagaaaaga cagggcgacc agcgccctga acagtgactt agccatgtgc
    21001 cagtttctgc accagtgact caaccatcag gctggactgt ttagcggcaa cagccaggaa
    21061 ctcatcgaag ctaagatgag actgttgatc ggccacgtcg gaaatggcgc gtacgacaac
    21121 aaacgggacg ttgaaattgt ggcagacatg ggcgattgcc gtcgcttcca tctctacagc
    21181 aatggcctgt gggaagttgt ggcggatttt cgccagacca acagaaccat tgatgaaagc
    21241 gtcgccgctg acaatcaggc cacgtacagc gtttagattc agttcggcaa tgcaggcctc
    21301 agcggcagcg atcagtttat cgtcagcttt aaagcctgcc gggcagcctg gtaactggcc
    21361 gtattcataa ccaaatgccg tgacatccgc gtcgtgataa cgcgcttcgt ccgagacaac
    21421 gatatcgccc actttcagtg ttggtgccag gccaccggca gaaccggtgt taataatcac
    21481 atctggcttg cagtgttcca acagcaaagt ggcacccagc gccgcagcga ctttaccgat
    21541 gcccgatttc agaagcgcaa cctcggttcc attcagttgg ccggtataga tttcgcaacc
    21601 gccgagactg atagtttgac ggttttcgat tttgtcacgc agtagcgtaa cttcttcttc
    21661 cattgcacca atgatgccga ttttcataga tttactcgcg ataagcccga tttgaaggca
    21721 tagtttacca tgcgcttacg gggaagcgta tttctcacgc gggagaggac atggcacaga
    21781 ttgatttccg aaaaaaaata aactggcatc gtcgttaccg ttcaccgcag ggcgttaaaa
    21841 ccgaacatga gatcctacgg atcttcgaga gcgatcgcgg gcgtatcatc aactctccgg
    21901 caattcgtcg tctgcaacaa aaaacccagg tttttccact ggagcgcaat gccgccgtgc
    21961 gcacgcgtct tacccactcg atggaagtcc agcaggtggg gcgctacatc gccaaagaaa
    22021 ttttaagccg tctgaaagag cttaaattac tggaagcata cggcctggat gaactgaccg
    22081 gcccctttga aagcattgtt gagatgtcat gccttatgca cgatatcggc aatccgccgt
    22141 ttggtcattt tggcgaagcg gcgataaatg actggtttcg ccagcgtttg cacccggaag
    22201 atgccgaaag ccagcctctg actgacgatc gctgcagcgt ggcagcacta cgtttacggg
    22261 acggggaaga accgcttaac gagctgcggc gcaaaatacg tcaggactta tgtcattttg
    22321 aggggaatgc acaaggcatt cgtctggtgc atacattgat gcggatgaat ctcacctggg
    22381 cacaggttgg cggtatttta aaatatactc gtccggcgtg gtggcgtggt gaaacgcctg
    22441 agacacatca ctatttaatg aaaaagccgg gttattatct ttctgaggaa gcctatattg
    22501 cccggttgcg taaagaactt aatttggcgc tttacagtcg ttttccatta acgtggatta
    22561 tggaagctgc cgacgacatc tcctattgtg tggcagacct tgaagatgcg gtagagaaaa
    22621 gaatatttac cgttgagcag ctttatcatc atttgcacga agcgtggggc cagcatgaga
    22681 aaggttcgct ctattcgctg gtggttgaaa atgcctggga aaaatcacgc tcaaatagtt
    22741 taagccgcag tacggaagat cagtttttta tgtatttacg ggtaaacacc ctaaataaac
    22801 tggtacccta cgcggcacaa cgatttattg ataatctgcc tgcgattttc gccggaacgt
    22861 ttaatcatgc attattggaa gatgccagcg aatgcagtga tcttctaaag ctatataaaa
    22921 atgtcgctgt aaaacatgta tttagccatc cagatgtcga gcagcttgaa ttgcagggct
    22981 atcgggtcat tagcggatta ttagagattt atcgcccttt attaagcctg tcgttatcag
    23041 actttactga actggtagaa aaagaacggg tgaaacgttt ccctattgaa tcgcgcttat
    23101 tcaacaaact ctcgacgcgc catcggctgg cctatgtcga ggctgtcagt aaattaccgt
    23161 cagattctcc tgagtttccg ctatgggaat attattaccg ttgccgcctg ctgcaggatt
    23221 atatcagcgg tatgaccgac ctctatgcgt gggatgaata ccgacgtctg atggccgtag
    23281 aacaataacc aggcttttgt aaagacgaac aataaatttt taccttttgc agaaacttta
    23341 gttcggaact tcgcgctata aaacgaatct gaagaacaca gcaattttgc gttatctgtt
    23401 aatcgagact gaaatacatg aaaaaaacca cattagcact gagtgcactg gctctgagtt
    23461 taggtttggc gttatctccg ctctctgcaa cggcggctga gacttcttca gcaacgacag
    23521 cccagcagat gccaagcctt gcaccgatgc tcgaaaaggt gatgccttca gtggtcagca
    23581 ttaacgtaga aggtagcaca accgttaata cgccgcgtat gccgcgtaat ttccagcagt
    23641 tcttcggtga tgattctccg ttctgccagg aaggttctcc gttccagagc tctccgttct
    23701 gccagggggg ccagggcggt aatggtggcg gccagcaaca gaaattcatg gcgctgggtt
    23761 ccggcgtcat cattgatgcc gataaaggct atgtcgtcac caacaaccac gttgttgata
    23821 acgcgacggt gattaaagtc cagctgagcg atggccgtaa gttcgacgcg aagatggttg
    23881 gcaaagatcc gcgctctgat atcgcgctga tccagatcca gaacccgaaa aatctgaccg
    23941 caattaagat ggcggattct gatgcactgc gcgtgggtga ttacaccgta gcaattggta
    24001 acccgtttgg tctgggcgag acggtaactt ccgggattgt ctctgcgctg gggcgtagcg
    24061 gcctgaatgc tgaaaactac gaaaacttca tccagaccga tgcagcgatc aaccgtggta
    24121 actccggtgg tgcgttggtt aacctgaacg gcgaactgat cggtatcaac accgcgatcc
    24181 tcgcaccgga cggcggcaac atcggtatcg gttttgctat cccgagtaac atggtgaaaa
    24241 acctgacctc gcagatggtg gaatacggcc aggtgaaacg cggtgagctg ggtattatgg
    24301 ggactgagct gaactccgaa ctggcgaaag cgatgaaagt tgacgcccag cgcggtgctt
    24361 tcgtaagcca ggttctgcct aactcctccg ctgcaaaagc gggcattaaa gcgggtgatg
    24421 tgatcacctc actgaacggt aagccaatca gcagctttgc cgccctgcgt gctcaggtgg
    24481 gtactatgcc ggtgggcagc aaactgaccc tgggcttact gcgcgacggt aagcaggtta
    24541 acgtgaacct ggaactgcag cagagcagcc agaatcaggt tgattccagc tccatcttca
    24601 acggcattga aggcgctgag atgagcaaca aaggcaaaga tcagggcgtg gtagtgaaca
    24661 acgtgaaaac gggcactccg gctgcgcaga tcggcctgaa aaaaggtgat gtgattattg
    24721 gcgcgaacca gcaggcagtg aaaaacatcg ctgaactgcg taaagttctc gacagcaaac
    24781 cgtctgtgct ggcactgaac attcagcgcg gcgacagcac catctacctg ttaatgcagt
    24841 aatctccctc aaccccttcc tgaatacggg aaggggttct ccttacaatc tgtgaacttc
    24901 gccacaactc catacatctt catcatcctt tgggcatttg cacaatgccg tacgttacgt
    24961 acttccttat gctaagccgt gcataacgga ggacttatgg ctggctggca tcttgatacc
    25021 aaaatggcgc aggatatcgt agcacgtacc atgcgcatca tcgataccaa tatcaacgta
    25081 atggatgccc gcgggcgaat tatcggcagc ggcgatcgtg agcgtattgg tgaattgcac
    25141 gaaggagcat tgctggtact ttcacaggga cgagtcgtcg atatcgatga cgcggtggca
    25201 cgtcatctgc acggtgtgcg gcaggggatt aatctaccgt tacggctgga aggtgaaatt
    25261 gtcggcgtaa ttggcctgac aggtgaacca gagaatctgc gtaaatatgg cgaactggtc
    25321 tgcatgacgg ctgaaatgat gctggaacag tcgcggttga tgcacctgtt ggcgcaggat
    25381 agccgtttgc gggaagaact ggtgatgaac ctcattcagg ctgaggagaa tactcccgca
    25441 cttactgaat gggcgcaacg gctggggatc gatctcaatc aaccgcgagt ggtggctatt
    25501 gttgaggtcg acagcggtca gcttggcgtg gacagcgcaa tggcggagtt acagcaactg
    25561 caaaatgcgc tgactacgcc cgagcgtaat aatctggtgg cgattgtctc gctaaccgaa
    25621 atggtggtgt tgaaaccggc gttgaactct tttgggcgct gggacgcaga agatcatcgt
    25681 aagagagttg aacaactgat tacccgcatg aaagagtatg gccagttgcg ttttcgcgtt
    25741 tcgctgggca actattttac cggtcctggc agtattgccc gatcctatcg tacggcgaaa
    25801 acgacgatgg tagtggggaa acagcggatg ccagaaagtc gctgctattt ttatcaggat
    25861 ctgatgttac ctgtgttact cgacagtttg cgtggcgact ggcaggccaa cgaactggcg
    25921 cgaccgctgg cgcggctgaa agcgatggat aataacggct tgctgcgacg tacgctggcg
    25981 gcgtggtttc gccataacgt gcagccgctg gcaacgtcaa aggcgctgtt tattcatcgt
    26041 aataccctgg agtatcggct taatcgtata tcggaactca caggcttaga tctggggaat
    26101 tttgatgaca ggttattgtt gtatgtggcg ttacaactgg atgaacagcg atagggtaaa
    26161 aatagtaaat gccgggaaat atgctcgtct taattcaagt tgcatgtacg gcgactcgaa
    26221 ttattcagcg tatactttac ccggctggca gaatggcaat atgcaaaatt atttacgtgt
    26281 cagcttttct aaatctgctt ctatctcgct gattttattg gtcaccactg actccaggtg
    26341 acgcaagtcg tcgagaattt tacgcttaag gtcaacttcg ctacgatcac gctggcagat
    26401 ttgatcaagc tcatcaatga tataacgaag attagggctg atttcctgaa cttctttata
    26461 tccctggcca ataccatcgg cgaccaccgt tttgcgctga cgcggatatt taaacttcac
    26521 actcttggcg aaaaactcgc ctttatcttt ctgaaaatag attttcaaaa tatcgttatt
    26581 ggcttcctgc cggaggctgt aacgatcaat ttcctcagga ttggtaatgc ccaaactttt
    26641 cagattgtcg tacatagttg tacctcaaaa gagtcagtaa ttgtgcttat cttagcattt
    26701 cgcctggcgg tttagctcga cggagatcgc aggaagcctt ttcttccaga cttttatcat
    26761 ggcctaaaaa tcaaaaaatt acctgcttta ttctgataag agaattcgct ttctacacca
    26821 gttccaggat tgtcacttcc gggcggcaat tcagacgcaa gccatacaaa cttccgacac
    26881 cccgagtcgt gtaaatttgt cgctcaccaa aggcatttaa accggcggcg tagcgtttat
    26941 cttctatggg cgcaaaaggc tcaccaacca acggtatgcg taactgcccg ccgtgggtgt
    27001 gaccacagag catcagatcc cacttttcat tacgcatgac ttctttgctg tcgggattat
    27061 gcgccagcac cagtcgtggc agatttgctt cgctggcggg aggcggtttg cattgtccgg
    27121 tccataaatc acccgtgcca accagttcaa attgctgctt ttgagtggtg attagcatgg
    27181 cttcgttaaa taacaccgtg atccccgccg attttaatgc ctcgccaatt aagcgatttt
    27241 tctctgtacc aacagggcga tcgtggttgc cgtaacaggc aaatgtcggc gcacattcgg
    27301 caagggagga gagtacgtca ctaaacgccg aaaaattcag ctgcatatca aataatacgt
    27361 aatcgccgcc cagcaatatc aaatcgggct tttgtgctac gccaagagca attgcgtcag
    27421 aaatcaggct taaaggaaca aaacgagagt aatggagatc tgccagaaaa aggattttga
    27481 aaggagcagc gttgtcttta aaaaaggcga ggcggtgacg gattaattca agccagccag
    27541 gttcccaata atgcatatag ctgaggcttg atccggcagc taacgtcgca gcggctgcct
    27601 gtaaaaaacg cctgcgtgtg atcatcatcc atccctgcaa aaaatcgggc agcgtcatgc
    27661 tgcccgtgta ctgattttta gtcgatggta cgcagcagtt cgttaatgcc gactttgccg
    27721 cgagttttcg cgtcaacttt cttaacgata actgcacaat aaaggctgta tttgccatct
    27781 ttcgacggca ggttgccaga aacaaccacc gaaccagccg gaacgcgacc gtagtggatt
    27841 tcgccggttt cacggtcgta aatacgggtg ctctgaccaa tgtatacgcc catagagatg
    27901 acggaacctt cttcgacaat caccccttca accacttcag agcgtgcacc gatgaagcaa
    27961 ttatcttcaa taatggtcgg gttagcctgc agcggctcca gcacgccgcc gatgcctaca
    28021 ccgccagaca gatggacgtt tttaccaatt tgtgcacagg aaccgacagt ggcccaggta
    28081 tcaaccatag tgccttcatc aacatatgcg ccgatgttga cgtaagacgg catcagcacg
    28141 gtgttacggg caataaatgc accctgacgt accgccgctg gtggcacaac gcggaagcct
    28201 tctttctgaa aacgcgcttc gtcgtagtcg gcgaatttca tcggcacttt atcgaagtag
    28261 cggctttctg ccccttcgat cacctgatta tcattaatac ggaaagagag cagcaccgct
    28321 tttttcaacc actgatgcgt cacccactga ccgtcaattt tttccgctac acgcagtgcg
    28381 ccggaatcca gcagggcgat cacctgattt accgcttcgc gggttacggt gtctgcattg
    28441 gctggcgtga tctcggcacg gcgttcaaaa gcggtttcaa taatgttctg taactgctgc
    28501 attgttaaac tcttttcata tcagtaaaca catcaccctt tatcgtttgg attgagggcc
    28561 tctgtcaacc gctgatgcac ttcctgctgc aactcgttat taagcgcacg ccggtcagcg
    28621 gtggcaatta tgaataaatc ttctactcgc tcgccaatgg ttgtaattcg ggcgccatga
    28681 agcgaaattc ccagatcggc aaaaattttc ccgactcgcg ccagcagtcc aggttggtcg
    28741 agggcgatca gttcgaggaa cgatttgcgg tcggtatggg tcggcaaaaa cgtcacttcg
    28801 gtttcaacag taaaatggcg caatttggcg ggttggcgac ggggctgcgg tggctgccag
    28861 ctactttgcg tcagcacctg ctccagacca aaccgaataa cctcatgacg atctgcggat
    28921 agcgggctac catcgggttc cagcacgatg aaggtatcca tcgccatacc gtcgcgagtg
    28981 gtgaaaattt gtgcgtcgtg aacacttaaa ttgcggcggt ctaattcggc acagacggcg
    29041 gcaaacagat aggggcggtc cgggctccag ataaaaatct cggtgcctcc acgcgtagcc
    29101 tgcggactaa gcaataccag cggtttgctt aaatcatgct gtaataaatg gcgcgcatgc
    29161 caggccagtt gatttgggct atggcggaca aaatagttag cacgacagcg tgaccaaatt
    29221 tggtgcagcg cctcttcgtc gatgttatcc atgcgcagta gtgccagcgc ctggagttgg
    29281 tgatggcgaa cccgttcgcg catatccggc gtgttttgca ttccgcgtcg tagctgcttt
    29341 tcggtggcga aatagagttc gcgcaacaga ctttgcttcc agctattcca cagcgtttcg
    29401 ttggtggcgc atatatcggc cactgtcagg cataccagat aacgaagtcg gttttctgtt
    29461 tgcacttctt cggcaaactg cttgatcact tccgggtcct gaatatcgcg gcgttgggcg
    29521 gtcaccgaca tcagcaagtg ctggcgaacc agccacgcca ccagctgtgt ttcgcgcgag
    29581 ttaagcccgt ggagttcggc aaaatgcacc acgtcttgtg caccgagaat ggagtggtcg
    29641 ccgccacgcc ctttggcgat atcgtgaaac agcgcggcga tgaaaatcag ctcagttgac
    29701 ggcaggcgcg gccagacgtc tacacacaac ggatggcgct ggcgcgtttc ttcactggca
    29761 aaactctcca gtttcagcat cacgcgaata gtatgttcat ccaccgtgta ggcgtggaac
    29821 aggtcaaact gcatctgccc gacgatatgc gaccattggg gcatatacgc gccgagcacg
    29881 ctatgacgat gcatcggtaa tagtccgcga cgcaccgcac cagggtgacg cagaatgctc
    29941 aaaaacagtt ttcgcgcttc cggaatatta cacagcggtt gttgcaaatg gcgacgggcg
    30001 tggcgtaact ggcgcagcgt ggtagagtaa atgccggtga tcgcactgtt gcgcaccatg
    30061 gtgtagaaca tacgcaagat ggcttccggc tggcgcataa atagtgtttc atcacgcagg
    30121 tcgatcagcg ttccgcgtag ctgaaactca tcgtcgattg gacgtggttt ttcgtcggcg
    30181 ggaagggcga ggatggcttc atcgaacagt tgcagcagca tctggttgag ttcactgacg
    30241 cggcgtgtaa cgcggaagta atccttcatc atccgctcga ccggttcgtt accttcgcca
    30301 ctgtaattca gacgctgggc gacgctaagc tggcgatcaa acagcaggcg attatcgtaa
    30361 cggctgacga ccagatgcag ggcaaagcga atacgccaca atatatgcag acattcgttt
    30421 aattccgctc gctccgctga ggttaagaag ccaaacccga ccatttcatc cagcgatgtt
    30481 gcgccaaaat gacggcgggc cacccattgc agagtgtgaa tatcgcgcaa gccgccagga
    30541 ctacttttga tgtctggttc aaggttgtag ctggtgccgt ggtaacgctg atggcgctgg
    30601 ttctgttctt caactttcgc tgcgtagaac ttgtcggaag gccagaatcc ttcgctaaaa
    30661 atatgttttt gcagttcgag gaagagcgct acatcgccaa ttaataagcg ggattcgatt
    30721 aaattggtgg cgacggttaa atccgataac ccttccagca tgcactcttc aagcgtgcgc
    30781 acgctatgac cgacttccag ctttacatcc cagagcagcg ttaacagctc gcccactttt
    30841 tgcgcctgat catccgggag ctttttacgg cttaaaatca gtaaatcgat gtcggaaagt
    30901 ggatgcagct cgccacgacc ataaccaccg acggcaacca atgccaggtc ggcaatctgg
    30961 ctgaatcccg cttcaatcca taatcgttgc aggagttggt cgataaactc ggtgcgtgcc
    31021 tcaatcaact gttctgcgga gatcccattg tcaaaggcat cacccagcca acgctggaaa
    31081 gtatcgatat gggcttttat cccacagacg gttaattcat cacggggcca ggcgcatgga
    31141 ttttgcggtt gaccgggcag ggtggggaga gcggtgtttg cgtactgttc tggaagggta
    31201 ttcattgtgc gccacccata agattaaatt atcgcattaa aaaagccggc attcgccggc
    31261 ttcatcttat tcgtcgtgcg agattatcgc cgggatggtg tcatccttgc gtagcgtcag
    31321 aatttcgcag ccgttatcag tcaccacaat agtatgctca tattgtgcag acaagctgcg
    31381 atctttggtt tttaccgtcc agccatcttt catggtgcgg atctcttttt tacccgcgtt
    31441 gaccattggc tcgatggtga acgtcatccc aggtttcagt acgacgttgg tttcacggga
    31501 gtcatagtgc agcacctgcg gttcttcgtg gaagccgcga ccaataccgt gtccacaata
    31561 ttcacgaacg acggagaagc cttctgcttc gacaaatttc tgaattgccg caccgatttc
    31621 gcgcagatta atgccaggtt ttaccatgcg tagcgccagg tacaggcttt cttgcgtgat
    31681 gcggcacaga cgttcgccca tgatggtcgg cttaccgacg ataaacattt tcgaggtatc
    31741 gccgtggaaa ccatctttga ttacggtgac atcaatgtta acgatatcgc catctttcag
    31801 cagcttggcg tcgtccggga taccgtggca caccacttca ttaatagaaa tgcaaacgga
    31861 tttcggatag ccgtgatagc cgaggcaggc agaaaccgcg tgttgttcat taacaatgta
    31921 atcattacag atgcgatcca gctcgccggt gctgacgccc ggtttaacat acggttcgat
    31981 catctccagc acttcggcag ccagtcggcc agcgacgcgc attttttcga tatcttctgg
    32041 ggtcttgatt gagatagcca ttaattctgt ccatcagcgt cggtgatacc gacaatatat
    32101 atgtaagtgc cgtcaatggt atcacacccg ggcaaattga gaatcattct gaatttcgcc
    32161 aaacgtgcca ctgaaggttt tctataatag aaaattcaac gtctgatgct gtacacagcg
    32221 ccaacaatta ttggtgtcca cgacgtattt gtggtataaa gcgcgccgga cttccgatcc
    32281 atttcgtata cacagactgg acggaagcga caatctcact ttgtgtaaca acacacacgt
    32341 atcggcacat attccggggt gccctttggg gtcggtaata tgggatacgt ggaggcataa
    32401 ccccaacttt tatatagagg ttttaatcat ggcaactgtt tccatgcgcg acatgctcaa
    32461 ggctggtgtt cacttcggtc accagacccg ttactggaac ccgaaaatga agccgttcat
    32521 cttcggtgcg cgtaacaaag ttcacatcat caaccttgag aaaactgtac cgatgttcaa
    32581 cgaagctctg gctgaactga acaagattgc ttctcgcaaa ggtaaaatcc ttttcgttgg
    32641 tactaaacgc gctgcaagcg aagcggtgaa agacgctgct ctgagctgcg accagttctt
    32701 cgtgaaccat cgctggctgg gcggtatgct gactaactgg aaaaccgttc gtcagtccat
    32761 caaacgtctg aaagacctgg aaactcagtc tcaggacggt actttcgaca agctgaccaa
    32821 gaaagaagcg ctgatgcgca ctcgtgagct ggagaaactg gaaaacagcc tgggcggtat
    32881 caaagacatg ggcggtctgc cggacgctct gtttgtaatc gatgctgacc acgaacacat
    32941 tgctatcaaa gaagcaaaca acctgggtat tccggtattt gctatcgttg ataccaactc
    33001 tgatccggac ggtgttgact tcgttatccc gggtaacgac gacgcaatcc gtgctgtgac
    33061 cctgtacctg ggcgctgttg ctgcaaccgt acgtgaaggc cgttctcagg atctggcttc
    33121 ccaggcggaa gaaagcttcg tagaagctga gtaataaggc ttgataactc ccccaaaata
    33181 gttcgagttg cagaaaggcg gcaagctcga gaattcccgg gagcttacat cagtaagtga
    33241 ccgggatgag cgagcgaaga taacgcatct gcggcgcgaa atatgaaggg ggagagccct
    33301 tatagaccag gtagtacacg tttggttagg gggcctgcat atggccccct ttttcacttt
    33361 tatatctgtg cggtttaatg ccgggcagat cacatctccg aggattttag aatggctgaa
    33421 attaccgcat ccctggtaaa agagctgcgt gagcgtactg gcgcaggcat gatggattgc
    33481 aaaaaagcac tgactgaagc taacggcgac atcgagctgg caatcgaaaa catgcgtaag
    33541 tccggtgcta ttaaagcagc gaaaaaagca ggcaacgttg ctgctgacgg cgtgatcaaa
    33601 accaaaatcg acggcaacta cggcatcatt ctggaagtta actgccagac tgacttcgtt
    33661 gcaaaagacg ctggtttcca ggcgttcgca gacaaagttc tggacgcagc tgttgctggc
    33721 aaaatcactg acgttgaagt actgaaagca cagttcgaag aagaacgtgt tgcgctggta
    33781 gcgaaaattg gtgaaaacat caacattcgc cgcgttgctg cgctggaagg cgacgttctg
    33841 ggttcttatc agcacggtgc gcgtatcggt gttctggttg ctgctaaagg cgctgacgaa
    33901 gagctggtta aacacatcgc tatgcacgtt gctgcaagca agccagaatt catcaaacca
    33961 gaagacgtat ccgctgaagt ggtagaaaaa gaataccagg tacagctgga tatcgcgatg
    34021 cagtctggta agccgaaaga aatcgcagag aaaatggttg aaggccgcat gaagaaattc
    34081 accggcgaag tttctctgac cggtcagccg ttcgttatgg aaccaagcaa aactgttggt
    34141 cagctgctga aagagcataa cgctgaagtg actggcttca tccgcttcga agtgggtgaa
    34201 ggcatcgaga aagttgagac tgactttgca gcagaagttg ctgcgatgtc caagcagtct
    34261 taattatcga aaaggagccg cctgagggcg gcttcttttt gtgcccatct tgtaaattca
    34321 gctaaccctt gtggggctgc gctgaaaagc gacgtacaat gtcgctagta ttaattcatt
    34381 tcaatcgttg acagtctcag gaaagaaaca tggctaccaa tgcaaaaccc gtctataaac
    34441 gcattctgct taagttgagt ggcgaagctc tgcagggcac tgaaggcttc ggtattgatg
    34501 caagcatact ggatcgcatg gctcaggaaa tcaaagaact ggttgaactg ggtattcagg
    34561 ttggtgtggt gattggtggg ggtaacctgt tccgtggcgc tggtctggcg aaagcgggta
    34621 tgaaccgcgt tgtgggcgac cacatgggga tgctggcgac cgtaatgaac ggcctggcaa
    34681 tgcgtgatgc actgcaccgc gcctatgtga acgctcgtct gatgtccgct attccattga
    34741 atggcgtgtg cgacagctac agctgggcag aagctatcag cctgttgcgc aacaaccgtg
    34801 tggtgatcct ctccgccggt acaggtaacc cgttctttac caccgactca gcagcttgcc
    34861 tgcgtggtat cgaaattgaa gccgatgtgg tgctgaaagc aaccaaagtt gacggcgtgt
    34921 ttactgctga tccggcgaaa gatccaaccg caaccatgta cgagcaactg acttacagcg
    34981 aagtgctgga aaaagagctg aaagtcatgg acctggcggc cttcacgctt gctcgtgacc
    35041 ataaattacc gattcgtgtt ttcaacatga acaaaccggg tgcgctgcgc cgtgtggtaa
    35101 tgggtgaaaa agaagggact ttaatcacgg aataattccc gtgatggata aataagggta
    35161 agattccgcg taagtatcgc gggggcgtaa gtctggttat aaggcgttat tgttgcaggc
    35221 agtttggtca cggccagcgc gcagcaaccg gagcgtacaa aagtacgtga ggatggcgag
    35281 cactgcccgg ggccaaaatg gcaaataaaa tagcctaata atccagacga ttacccgtaa
    35341 tatgtttaat cagggctata cttagcacac ttccactgtg tgtgactgtc tggtctgact
    35401 gagacaagtt ttcaaggatt cgtaacgtga ttagcgatat cagaaaagat gctgaagtac
    35461 gcatggataa atgcgtagaa gcgttcaaaa cccaaatcag caaaatacgc acgggtcgtg
    35521 cttctcccag cctgctggat ggcattgtcg tggaatatta cggcacgccg acgccgctgc
    35581 gtcagctggc aagcgtaacg gtagaagatt cccgtactct gaaaatcaac gtgtttgatc
    35641 gttcaatgtc tccggccgtt gaaaaagcga ttatggcgtc cgatcttggc ctgaacccga
    35701 actctgcggg tagcgacatc cgtgttccgc tgccgccgct gacggaagaa cgtcgtaaag
    35761 atctgaccaa aatcgttcgt ggtgaagcag aacaagcgcg tgttgcagta cgtaatgtgc
    35821 gtcgtgacgc gaacgacaaa gtgaaagcac tgttgaaaga taaagagatc agcgaagacg
    35881 acgatcgccg ttctcaggac gatgtacaga aactgactga tgctgcaatc aagaaaattg
    35941 aagcggcgct ggcagacaaa gaagcagaac tgatgcagtt ctgatttctt gaacgacaaa
    36001 aacgccgctc agtagatcct tgcggatcgg ctggcggcgt tttgcttttt attctgtctc
    36061 aactctggat gtttcatgaa gcaactcacc attctgggct cgaccggctc gattggttgc
    36121 agcacgctgg acgtggtgcg ccataatccc gaacacttcc gcgtagttgc gctggtggca
    36181 ggcaaaaatg tcactcgcat ggtagaacag tgcctggaat tctctccccg ctatgctgta
    36241 atggatgatg aagcgagtgc gaaacttctt aaaacgatgc tacagcaaca gggtagccgc
    36301 accgaagtct taagtgggca acaagccgct tgcgatatgg cagcgcttga ggatgttgat
    36361 caggtgatgg cagccattgt tggcgctgct gggctgttac ctacgcttgc tgcgatccgc
    36421 gcgggtaaaa ccattttgct ggccaataaa gaatcactgg ttacctgcgg acgtctgttt
    36481 atggacgccg taaagcagag caaagcgcaa ttgttaccgg tcgatagcga acataacgcc
    36541 atttttcaga gtttaccgca acctatccag cataatctgg gatacgctga ccttgagcaa
    36601 aatggcgtgg tgtccatttt acttaccggg tctggtggcc ctttccgtga gacgccattg
    36661 cgcgatttgg caacaatgac gcccgatcaa gcctgccgtc atccgaactg gtcgatggga
    36721 cgtaaaattt ctgtcgattc ggctaccatg atgaacaaag gtctggaata cattgaagcg
    36781 cgttggctgt ttaacgccag cgccagccag atggaagtgc tgattcaccc gcagtcagtg
    36841 attcactcaa tggtgcgcta tcaggacggc agtgttctgg cgcagctggg ggaaccggat
    36901 atgcgtacgc caattgccca caccatggca tggccgaatc gcgtgaactc tggcgtgaag
    36961 ccgctcgatt tttgcaaact aagtgcgttg acatttgccg caccggatta tgatcgttat
    37021 ccatgcctga aactggcgat ggaggcgttc gaacaaggcc aggcagcgac gacagcattg
    37081 aatgccgcaa acgaaatcac cgtagctgct tttcttgcgc aacaaatccg ctttacggat
    37141 atcgccgcgt tgaatttatc cgtactggaa aaaatggata tgcgcgaacc acaatgtgtg
    37201 gacgatgtgt tatctgttga tgcgaacgcg cgtgaagtcg ccagaaaaga ggtgatgcgt
    37261 ctcgcaagct gaggataatc cggctacaga gagtcgcgct atttgttagc gtagggcttc
    37321 agtgatatag tctgcgccat ctgatcgtaa gtagttggct ttataaggtc aggtatgccg
    37381 tggttttaca cggctttttt ttgtataggc ttcagtattc ctgagtaccg taaaccctgt
    37441 cagggaataa gaaacgcgtg atgttgtctg ctactcaacc acttagcgaa aaattgccag
    37501 cgcatggctg tcgtcatgtt gcgatcatta tggacggcaa tggtcgctgg gcaaaaaagc
    37561 aagggaagat tcgtgccttt gggcataaag ccggggcaaa atccgtccgc cgggctgtct
    37621 cttttgcggc caacaacggt attgaggcgt taacgctgta tgcctttagt agtgaaaact
    37681 ggaaccgacc agcgcaggaa gtcagtgcgt taatggaact gtttgtgtgg gcgctcgata
    37741 gcgaagtaaa aagtctgcac cgacataacg tgcgtctgcg tattattggc gataccagtc
    37801 gctttaactc gcgtttgcaa gaacgtattc gtaaatctga agcgctaaca gccgggaata
    37861 ccggtctgac gctgaatatt gcggcgaact acggtggacg ttgggatata gtccagggag
    37921 tcaggcaact ggctgaaaag gtgcagcaag gaaacctgca accagatcag atagatgaag
    37981 agatgctaaa ccagcatgtc tgtatgcatg aactggcccc tgtagattta gtaattagga
    38041 ctggggggga gcatcgcatt agtaactttt tgctttggca aattgcctat gccgaacttt
    38101 actttacaga tgttctctgg cccgatttcg atgaacaaga ctttgaaggg gcgttaaatg
    38161 cctttgctaa tcgagagcgt cgtttcggcg gcaccgagcc cggtgatgaa acagcctgat
    38221 gggggtcgct tttgctgaag tatcgcctga tatctgcttt tgtgttaata cccgttgtca
    38281 tcgcggcgtt gtttctgttg ccgccggtgg ggttcgccat tgtaacgctg gtggtctgca
    38341 tgctggcagc gtgggaatgg ggacagctta gcggttttac cactcgttcg cagcgagtat
    38401 ggttggcggt gttatgcggg ttattgttgg cgctgatgct ttttctgttg ccggaatatc
    38461 accgaaatat tcatcaaccg ctggttgaaa tctcactttg ggcttcgctg ggttggtgga
    38521 ttgtcgcgct attgctggtg ctgttttacc ctggttccgc agcaatctgg cgtaactcta
    38581 aaacattgcg ccttattttt ggcgtgctaa ccattgttcc cttcttctgg ggcatgctgg
    38641 cgttacgggc ctggcactac gacgagaatc attacagtgg cgcaatatgg ctgctctatg
    38701 tcatgatcct ggtatggggc gctgactctg gcgcatatat gtttggcaaa ttgtttggta
    38761 aacataagct ggcaccgaag gtttctccgg gtaaaacctg gcaaggcttt ataggtggac
    38821 tcgcgaccgc agcggtaatc tcatggggtt atggcatgtg ggcgaatctc gacgtcgctc
    38881 ccgtcacctt actcatttgc tctattgtcg cagcgttagc ctcagtgctc ggcgatctga
    38941 ccgagagtat gtttaagcgt gaagcaggaa ttaaggacag cggtcattta attccaggac
    39001 acggtggtat tttagatcgt attgatagcc tgacggctgc ggtaccggtc tttgcttgct
    39061 tgttgttact ggtattcagg acgctttaac ggaaggtaat atgctgagtt ttctctggga
    39121 tttggcttcg ttcatcgttg cactgggtgt acttatcacc gtgcatgaat ttggtcattt
    39181 ctgggttgcc cggcgttgtg gtgttcgcgt tgagcgtttc tcaatagggt ttggtaaggc
    39241 gctctggcgg cgaactgata agctcggcac cgaatatgtt atcgccctga tcccgctggg
    39301 cggttatgtc aaaatgctgg atgagcgcgc agaaccggtc gttccggaac tccgccacca
    39361 tgccttcaat aataaatctg tcggccaacg agcggcgatt attgccgcag gtccggttgc
    39421 aaacttcatt tttgctatct ttgcctactg gctggttttt attattggtg tgcctggcgt
    39481 acgtccggtg gttggcgaaa tagcagccaa ttcgatagct gcggaagcac aaattgcacc
    39541 aggtacggaa ctaaaagccg tagatggtat cgaaacgcct gattgggatg ccgtgcgttt
    39601 gcagttggtc gataaaattg gcgatgaaag caccaccatt acggtagcgc catttggcag
    39661 cgaccaacgg cgggatgtaa agctcgattt acgtcactgg gcgtttgagc ctgataaaga
    39721 agatccggta acttcgctgg ggattcgtcc tcgtgggccg caaattgaac ctgtactgga
    39781 aaatgtgcag ccaaactcgg cggcaagcaa ggcaggtttg caagcaggcg acaggatcgt
    39841 taaagtcgat ggtcagccct taacgcagtg ggtgaccttt gtgatgcttg tccgggataa
    39901 cccgggtaaa tccttagcgt tagaaatcga aaggcagggg agtcccttgt ctttgacatt
    39961 aatcccggag agtaaaccgg gtaatggtaa agcgattggt tttgtcggta ttgagccgaa
    40021 agtcattcct ttgccagatg agtataaagt tgtacgccag tatgggccgt tcaacgccat
    40081 cgtcgaagcc acggacaaaa cgtggcagct gatgaagctg acggtcagta tgctgggaaa
    40141 attgatcacc ggtgatgtga aactgaacaa cctcagtggg ccgatctcta tcgccaaggg
    40201 ggctgggatg acagcggaac tcggggttgt ttattacctg ccgtttcttg cgcttattag
    40261 cgtgaactta gggataatta acctgtttcc gttgcccgta cttgacgggg ggcatctgct
    40321 gttccttgcg atcgaaaaga tcaagggcgg accggtatcc gagcgggttc aagacttttg
    40381 ttatcgcatt ggctcgattt tgctggtgct gttaatgggg cttgcacttt tcaatgattt
    40441 ctctcggtta tgagagttag ttaggaagaa cgcataataa cgatggcgat gaaaaagttg
    40501 ctcatagcgt cgctgctgtt tagcagcgcc accgtatacg gtgctgaagg gttcgtagtg
    40561 aaagatattc atttcgaagg ccttcagcgt gtcgccgttg gtgcggccct cctcagtatg
    40621 ccggtgcgca caggcgacac ggttaatgat gaagatatca gtaataccat tcgcgctctg
    40681 tttgctaccg gcaactttga ggatgttcgc gtccttcgtg atggtgatac ccttctggtt
    40741 caggtaaaag aacgtccgac cattgccagc attactttct ccggtaacaa atcggtgaaa
    40801 gatgacatgc tgaagcaaaa cctcgaggct tctggcgtgc gtgtgggcga atccctcgat
    40861 cgcaccacca ttgccgatat cgagaaaggt ctggaagact tctactacag cgtcggtaaa
    40921 tatagcgcca gcgttaaagc tgtcgtgacc ccgctgccgc gcaaccgtgt tgacctaaaa
    40981 ctggtgttcc aggaaggtgt gtcagctgaa atccagcaaa ttaacattgt tggtaaccat
    41041 gctttcacca ccgacgaact gatctctcat ttccaactgc gtgacgaagt gccgtggtgg
    41101 aacgtggtag gcgatcgtaa ataccagaaa cagaaactgg cgggcgacct tgaaaccctg
    41161 cgcagttact atctggatcg cggttatgcc cgtttcaaca tcgactctac ccaggtcagt
    41221 ctgacgcctg ataaaaaagg tatttacgtc acggtgaaca tcaccgaagg cgatcagtac
    41281 aagctttctg gcgttgaagt gagcggcaac cttgccgggc actccgctga aattgagcag
    41341 ctgactaaga tcgagccggg cgagctgtat aacggcacca aagtgaccaa aatggaagat
    41401 gacattaaaa agcttcttgg tcgctatggt tatgcctatc cgcgcgtaca gtcgatgcct
    41461 gaaattaacg atgccgacaa aaccgttaaa ttacgtgtga acgttgatgc gggtaaccgt
    41521 ttctacgtgc gtaagatccg ttttgaaggt aacgatacct cgaaagatgc cgtcctgcgt
    41581 cgcgaaatgc gtcagatgga aggtgcatgg ctggggagcg atctggtcga tcagggtaag
    41641 gagcgtctga atcgtctggg cttctttgaa actgtcgata ccgataccca acgcgttccg
    41701 ggtagcccgg accaggttga tgtagtctac aaggtgaaag aacgtaacac cggtagcttc
    41761 aacttcggga ttggttacgg tactgaaagt ggcgtgagct tccaggctgg tgtgcaacag
    41821 gataactggt taggtacggg ttatgctgtt ggtatcaacg ggaccaaaaa cgattaccag
    41881 acctatgctg aactgtcggt aaccaacccg tacttcaccg tagatggtgt aagcctcggt
    41941 ggtcgtctct tctataatga cttccaggca gatgacgccg acctgtccga ctataccaac
    42001 aagagttatg gtacagacgt gacgttgggc ttcccgatca acgaatataa ctcgctgcgt
    42061 gcaggtctgg gttatgtaca taactccctg tccaacatgc agcctcaggt tgcgatgtgg
    42121 cgttatctgt actctatggg tgaacatccg agcacctctg atcaggataa cagcttcaaa
    42181 acggacgact tcacgttcaa ctatggttgg acctataaca agcttgaccg tggttacttc
    42241 ccgacagatg gttcacgtgt caacctgacc ggtaaagtga ccattccggg atcggataac
    42301 gaatactaca aagtgacgtt agacacggcg acttatgtgc cgattgatga cgatcacaaa
    42361 tgggttgttc tgggtcgtac ccgctggggt tatggtgatg gtttaggcgg caaagagatg
    42421 ccgttctatg agaacttcta tgccggtggt tccagcaccg tgcgtggctt ccagtccaat
    42481 accattggtc cgaaagcagt ttacttcccg catcaggcca gtaattatga tccggactat
    42541 gattacgaat gtgcgactca ggacggcgcg aaagatctgt gtaaatcgga tgatgctgta
    42601 ggcggtaacg ccatggcggt tgccagcctc gagttcatca ccccgacgcc gtttattagc
    42661 gataagtatg ctaactcggt tcgtacttcc ttcttctggg atatgggtac cgtttgggat
    42721 acaaactggg attccagcca atattctgga tatccggact atagtgatcc aagcaatatc
    42781 cgtatgtctg cgggtatcgc attacaatgg atgtccccat tggggccgtt ggtgttctcc
    42841 tacgcccagc cgttcaaaaa gtacgatgga gacaaggcag aacagttcca gtttaacatc
    42901 ggtaaaacct ggtaagtgtt ctccacaaag gaatgtagtg gtagtgtagc gatgacttta
    42961 ggcgatcaat ataagatcgc cgggccacgc aaagaactgc accctccggt gcaaatggga
    43021 tggtaaggag tttattgtga aaaagtggtt attagctgca ggtctcggtt tagcactggc
    43081 aacttctgct caggcggctg acaaaattgc aatcgtcaat atgggcagcc tgttccagca
    43141 ggtagcgcag aaaaccggag tttctaacac gctggaaaat gagttcaaag gccgtgccag
    43201 cgaactgcag cgtatggaaa ccgatctgca ggctaaaatg aaaaagctgc agtccatgaa
    43261 agcgggcagc gatcgcacca aactggaaaa agacgtgatg gcccagcgcc agacttttgc
    43321 tcagaaagcg caggcttttg agcaggatcg cgcacgtcgt tccaacgaag aacgcggcaa
    43381 actggttact cgtatccaga ctgctgtgaa atccgttgcc aacagccagg atatcgatct
    43441 ggttgttgat gcaaacgccg ttgcttacaa cagcagcgat gtaaaagaca tcactgccga
    43501 cgtactgaaa caggttaaat aagtaatgcc ttcaattcga ctggctgatt tagcgcagca
    43561 gttggatgca gaactacacg gtgatggcga tatcgtcatc accggcgttg cgtccatgca
    43621 atctgcacaa acaggtcaca tcacgttcat ggttaaccca aaataccgtg agcatttagg
    43681 cttgtgccag gcgtccgcgg ttgtcatgac ccaggacgat cttcctttcg cgaaaagtgc
    43741 cgcgctggta gtgaagaatc cctacctgac ttacgcgcgc atggcgcaaa ttttagatac
    43801 cacgccgcag cccgcgcaga acattgcacc cagtgcggtg attgacgcga cggcgaagct
    43861 gggtaacaac gtatcgattg gcgctaacgc ggtgattgag tccggcgttg aactgggcga
    43921 taacgtgatt atcggtgccg gttgcttcgt aggtaaaaac agcaaaatcg gtgcaggttc
    43981 gcgtctctgg gcgaacgtaa ccatttacca tgagatccag atcggtcaga attgcctgat
    44041 ccagtccgga acagtggtag gcgcagacgg ctttggttat gccaacgatc gtggtaactg
    44101 ggtgaagatc ccacagattg gtcgcgtaat tattggcgat cgcgtggaga tcggtgcctg
    44161 cactaccatt gatcgtggcg cgctggatga cactgttatt ggcaatggtg tgatcattga
    44221 taaccagtgc cagattgcac ataacgtcgt gattggcgac aatacggcag ttgccggtgg
    44281 cgtcattatg gcgggcagcc tgaaaattgg tcgttactgc atgatcggcg gagccagcgt
    44341 aatcaacggg catatggaaa tatgcgacaa agtgacggtt acgggcatgg gtatggtgat
    44401 gcgtcccatc actgaaccag gcgtctattc ctcaggcatt ccgctgcaac ccaacaaagt
    44461 ctggcgcaaa accgctgcac tggtgatgaa cattgatgac atgagcaagc gtctgaaatc
    44521 gcttgagcgc aaggttaatc aacaagacta acgttccatc ttttgttcgc caaactttac
    44581 ggcctgtctc attcttacga ttgcggcagg ccgtgttatt attgtcgttt cttatatttt
    44641 gacaggaaga gtatcttgac tactaacact catactctgc agattgaaga gattttagaa
    44701 cttctgccgc accgtttccc gttcttactg gtggatcgcg tgctggattt tgaagaaggt
    44761 cgttttctgc gcgcagtaaa aaatgtctct gtcaatgagc cattcttcca gggccatttc
    44821 cctggaaaac cgattttccc gggtgtgctg attctggaag caatggcaca ggcaacaggt
    44881 attctggcgt ttaaaagcgt aggaaaactg gaaccgggtg agctgtacta cttcgccggt
    44941 attgacgaag cgcgctttaa gcgcccggtc gtgcctggcg atcaaatgat catggaagtc
    45001 actttcgaaa aaacgcgccg cggcctgacc cgttttaaag gggttgctct ggtcgacggt
    45061 aaagtagttt gcgaagcaac gatgatgtgt gctcgtagcc gggaggcctg atacgtgatt
    45121 gataaatccg cctttgtgca tccaaccgcc attgtggaag agggcgcgtc gattggcgcg
    45181 aacgctcaca ttggtccttt ttgtatcgtt ggaccccatg tcgaaattgg tgagggtacc
    45241 gtactgaaat ctcacgttgt cgtgaatggt catactaaaa ttggccgcga caatgagatt
    45301 tatcagttcg cctccatcgg cgaagtcaac caggatctta aatatgctgg cgaaccgacc
    45361 cgtgtggaaa tcggcgatcg taaccgcatt cgcgaaagcg tcaccattca tcgtggcaca
    45421 gtccagggcg gtggattgac gaaggtgggc agcgacaact tactgatgat caacgcgcac
    45481 attgcgcacg attgtacggt aggtaaccgc tgtattctcg ccaacaacgc aacgctggcg
    45541 ggtcacgtat cggttgacga cttcgcgatc atcggcggca tgaccgcagt ccatcagttc
    45601 tgcatcattg gtgcgcacgt gatggttggc ggctgctccg gtgtggcgca ggacgtccct
    45661 ccttatgtca ttgcgcaggg taaccacgca acgccgttcg gtgtcaatat cgaagggctg
    45721 aagcgccgcg gattcagccg tgaggcgatt accgctatcc gcaatgcgta taagctgatt
    45781 tatcgtagcg gtaaaacgct cgatgaagtg aaaccggaaa ttgctgaact ggcggaaaca
    45841 tatccggaag tgaaagcctt taccgatttc tttgcacgct caacgcgcgg tctgattcgt
    45901 taatgactga acagcgtcca ttaacgattg ccctggtcgc cggagaaacc tccggcgata
    45961 tcctgggggc cggtttaatc cgcgctctga aagaacgtgt gcccaacgcc cgctttgttg
    46021 gtgttgccgg gccacgaatg caggctgaag gctgcgaagc ctggtacgaa atggaagaac
    46081 tggcggtgat gggcattgtt gaagtgctcg gtcgtctgcg tcgcttactg catattcgtg
    46141 ccgatctgac aacgcgtttt ggcgaactga agccagatgt ttttgtcggt attgatgcgc
    46201 ctgacttcaa tattaccctt gaaggtaacc ttaaaaagca gggtatcaaa accattcatt
    46261 atgtcagtcc gtccgtctgg gcgtggcgac agaaacgcgt tttcaaaata ggcagagcca
    46321 ccgatctggt gctcgcattt ctgcctttcg aaaaagcgtt ttatgacaaa tataacgtgc
    46381 cgtgccgttt tatcggtcac accatggctg atgccatgcc gttagatcca gataaaaacg
    46441 ccgcgcgtga tgtgctgggg atccctcacg atgcccactg tctggcattg ttgccgggaa
    46501 gccgtggcgc ggaagtggag atgcttagcg ccgatttcct gaaaacggcc cagcttttgc
    46561 gccagacgta tcctgatctg gagatcgtgg tgccgctggt aaatgccaaa cgccgtgagc
    46621 aatttgaacg catcaaagct gaagtcgcgc cagacctttc ggttcatatg ctggatggaa
    46681 tgggccgtga ggcgatggtc gccagcgatg cggcgctatt ggcgtcgggt acggctgcac
    46741 tggagtgcat gctggcgaaa tgcccgatgg tggtggggta tcgcatgaag cctttcacct
    46801 tctggttggc gaagcggctg gtgaaaaccg attatgtctc gctgccaaac ctgctggcgg
    46861 gcagagagtt agttaaagaa ttattgcagg aagagtgtga gccgcaaaaa ctggctgcgg
    46921 cgctgttacc gctgttggcg aacgggaaaa ccagccacgc gatgcacgat accttccgtg
    46981 aactgcatca gcagatccgc tgcaatgccg atgagcaggc ggcacaagcc gttctggagt
    47041 tagcacaatg atcgaatttg tttatccgca cacgcacctg gttgcgggtg tggatgaagt
    47101 cggacgcggg ccgttagttg gcgcggtcgt caccgctgcg gtgatcctcg acccggcgcg
    47161 cccgattgcc gggctgaatg attccaaaaa gctgagcgaa aaacgccgtc tggcgctctg
    47221 cgaagagatc aaagagaaag cgttgagctg gagtctgggc cgagcggaac ctcacgaaat
    47281 cgacgaactg aatattttac acgcgaccat gctggcgatg cagcgtgccg tcgctgggct
    47341 gcatattgcg ccggaatatg tgttgattga tggtaaccgc tgcccgaaat taccgatgcc
    47401 tgcgatggct gtggtgaaag gcgatagccg cgtaccggaa atcagtgccg cgtctatcct
    47461 ggcgaaagtg acgcgtgacg ccgaaatggc ggcgctggat attgttttcc cgcaatatgg
    47521 ttttgcccaa cataaagggt atccaaccgc ttttcatctg gaaaaactgg ctgaatatgg
    47581 cgcgactgaa catcatcgtc gcagctttgg gcctgtcaaa cgcgtactgg gacttgcgtc
    47641 ctgattcttg tgtcgagatt aagtaaaccg gaatctgaag atgtctgaac cacgtttcgt
    47701 acacctgcgg gtgcacagcg actactcgat gatcgatggt ctggccaaaa ccgcaccgct
    47761 ggtaaaaaag gcggcggcgt tgggtatgcc agcactggcg atcaccgatt tcaccaacct
    47821 ttgcggtctg gtgaagttct acggagcggg acatggcgca gggattaagc ctatcgtcgg
    47881 ggcagatttt aatgtccagt gcgacctgct gggtgatgag ttaacccacc tgacggtgct
    47941 ggcggcgaac aataccggct atcagaatct gacgttgctg atctcaaaag cgtatcagcg
    48001 cgggtacggt gcagctgggc cgatcatcga tcgcgactgg cttatcgaat taaacgaagg
    48061 gttgatcctt ctttccggcg ggcgcatggg cgacgtcgga cgcagtcttt tgcgtggtaa
    48121 cagcgcgctg gtagatgagt gtgtcgcgtt ttatgaagaa cacttcccgg atcgctattt
    48181 tctcgagctg atccgcaccg gcaggccgga cgaagaaagc tatctgcacg ctgcggtgga
    48241 actggcggaa gcacgcggtt tgcccgttgt ggcgaccaac gacgtgcgct ttatcgacag
    48301 cagcgacttt gacgcacacg aaatccgcgt cgcgatccac gacggcttta ccctcgacga
    48361 tcctaaacgc ccgcgtaact attcgccgca gcaatatatg cgtagcgaag aggagatgtg
    48421 cgagcttttt gccgacattc ccgaagccct tgccaacacc gttgagatcg ccaaacgttg
    48481 taacgtaacc gtgcgtcttg gtgaatactt cctgccgcag ttcccgaccg gggacatgag
    48541 caccgaagat tatctggtca agcgtgcaaa agaggggctg gaagagcgtc tggccttttt
    48601 attccctgac gaggaagaac gtgttaagcg ccgcccggaa tatgacgaac gtctggagac
    48661 tgaacttcag gttatcaacc agatgggctt cccgggttac ttcctcatcg ttatggaatt
    48721 tatccagtgg tcgaaagata acggcgtacc agtagggcca ggccgtggct ccggtgcggg
    48781 ttcactggtg gcctacgcgc tgaaaatcac cgacctcgat ccgctggaat ttgacctgct
    48841 gttcgaacgt ttccttaacc cggaacgtgt ctccatgcct gacttcgacg ttgacttctg
    48901 tatggagaaa cgcgaccagg ttatcgagca cgtagcggac atgtacggtc gtgatgcggt
    48961 atcgcagatc atcaccttcg gtacgatggc ggcgaaagcg gtgatccgcg acgtaggtcg
    49021 cgtgctgggg catccgtacg gctttgtcga tcgtatctcg aaactgatcc cgcccgatcc
    49081 ggggatgacg ctggcgaaag cgtttgaagc cgagccgcag ctgccggaaa tctacgaagc
    49141 ggatgaagaa gttaaggcgc tgatcgacat ggcgcgcaaa ctggaagggg tcacccgtaa
    49201 cgccggtaag cacgccggtg gggtggttat cgcgccgacc aaaattaccg attttgcgcc
    49261 gctttactgc gatgaagagg gcaaacatcc ggtcacccag tttgataaaa gcgacgttga
    49321 atacgccgga ctggtgaagt tcgacttcct tggtttgcgt acgctcacca tcatcaactg
    49381 ggcgctggag atgatcaaca agcggcgggc gaagaatggc gagccgccgc tggatatcgc
    49441 cgcgatcccg ctggatgata agaaaagctt cgacatgctg caacgctcgg aaaccactgc
    49501 ggtattccag cttgaatcgc gcggcatgaa ggacctgatc aagcgtctgc aacctgactg
    49561 cttcgaagat atgatcgcac tggtggcact gttccgcccc ggtccgttgc aatcagggat
    49621 ggtggataac tttatcgacc gtaaacatgg tcgcgaagag atctcctatc cggacgtgca
    49681 gtggcagcat gaaagcctga aaccggtact ggagccaacc tacggcatca tcctgtatca
    49741 ggaacaggtc atgcagattg cccaggtgct ttctggttat accctcggtg gcgcagatat
    49801 gctgcgtcgt gcgatgggta agaaaaagcc ggaagagatg gctaagcagc gttctgtatt
    49861 tgctgaaggt gcagaaaaga acggaatcaa cgccgaactg gcgatgaaaa tcttcgacct
    49921 ggtggagaaa ttcgcgggtt acggatttaa caaatcgcac tctgcggcct atgctttggt
    49981 gtcatatcaa acgttatggc tgaaagcgca ctatccggca gagtttatgg cggcggtaat
    50041 gaccgccgat atggacaata ccgagaaggt ggtgggcctg gtggatgagt gctggcggat
    50101 ggggctgaaa atcctgccac cagatataaa ctccggtctt taccatttcc acgtcaacga
    50161 cgacggcgaa atcgtgtatg gtattggcgc gatcaaaggg gtaggtgaag gtccgattga
    50221 ggccatcatc gaagcccgta ataaaggcgg ctacttccgc gaactgtttg atctctgcgc
    50281 ccgaaccgac accaaaaagt taaaccggcg ggtgctggaa aaactgatca tgtccggggc
    50341 gtttgaccgt cttgggccac accgtgcggc gctgatgaac tcgctgggcg atgcgttaaa
    50401 agcggcagat caacacgcga aagcggaagc tatcggtcag gccgatatgt tcggcgtgct
    50461 ggccgaagaa ccggaacaaa ttgaacaatc ctacgccagc tgccaaccgt ggccggagca
    50521 ggtggtatta gatggggaac gtgaaacgtt aggtctgtac ctgacgggac accctatcaa
    50581 ccagtatcta aaagagattg agcgttatgt cggaggcgta aggctgaaag acatgcaccc
    50641 gacagaacgt ggtaaagtca tcacggctgc ggggctcgtt gttgccgcgc gggttatggt
    50701 caccaagcgc ggcaatcgta tcggtatctg cacgctggat gaccgttccg ggcggctgga
    50761 agtgatgttg tttactgacg ccctggataa ataccagcaa ttgctggaaa aagaccgcat
    50821 acttatcgtc agcggacagg tcagctttga tgacttcagc ggtgggctta aaatgaccgc
    50881 tcgcgaagtg atggatattg acgaagcccg ggaaaaatat gctcgcgggc ttgctatctc
    50941 gctgacggac aggcaaattg atgaccagct tttaaaccga ctccgtcagt ctctggaacc
    51001 ccaccgctct gggacaattc cagtacatct ctactatcag agggcggatg cacgcgcgcg
    51061 gttgcgtttt ggcgcgacgt ggcgtgtctc tccgagcgat cgtttattaa acgatctccg
    51121 tggcctcatt ggttcggagc aggtggaact ggagtttgac taatacagga atactatgag
    51181 tctgaatttc cttgattttg aacagccgat tgcagagctg gaagcgaaaa tcgattctct
    51241 gactgcggtt agccgtcagg atgagaaact ggatattaac atcgatgaag aagtgcatcg
    51301 tctgcgtgaa aaaagcgtag aactgacgcg taaaatcttc gccgatctcg gtgcatggca
    51361 gattgcgcaa ctggcacgcc atccacagcg tccttatacc ctggattacg ttcgcctggc
    51421 atttgatgaa tttgacgaac tggctggcga ccgcgcgtat gcagacgata aagctatcgt
    51481 cggtggtatc gcccgtctcg atggtcgtcc ggtgatgatc attggtcatc aaaaaggtcg
    51541 tgaaaccaaa gaaaaaattc gccgtaactt tggtatgcca gcgccagaag gttaccgtaa
    51601 agcactgcgt ctgatgcaaa tggctgaacg ctttaagatg cctatcatca cctttatcga
    51661 caccccgggg gcttatcctg gcgtgggcgc agaagagcgt ggtcagtctg aagccattgc
    51721 acgcaacctg cgtgaaatgt ctcgcctcgg cgtaccggta gtttgtacgg ttatcggtga
    51781 aggtggttct ggcggtgcgc tggcgattgg cgtgggcgat aaagtgaata tgctgcaata
    51841 cagcacctat tccgttatct cgccggaagg ttgtgcgtcc attctgtgga agagcgccga
    51901 caaagcgccg ctggcggctg aagcgatggg tatcatcgct ccgcgtctga aagagctgaa
    51961 actgatcgac tccatcatcc cggaaccgct gggcggcgct catcgtaacc cggaagcgat
    52021 ggcggcatcg ttgaaagcgc aactgctggc ggatctggcc gatctcgacg tgttaagcac
    52081 tgaagattta aaaaatcgtc gttatcagcg cctgatgagc tacggttacg cgtaattcgc
    52141 aaaagttctg aaaaagggtc acttcggtgg ccctttttta tcgccacggt ttgagcaggc
    52201 tatgattaag gaaggatttt ccaggaggaa cacatgaaca tcattgccat tatgggaccg
    52261 catggcgtct tttataaaga tgagcccatc aaagaactgg agtcggcgct ggtggcgcaa
    52321 ggctttcaga ttatctggcc acaaaacagc gttgatttgc tgaaatttat cgaacataac
    52381 cctcgaattt gcggcgtgat ttttgactgg gatgagtaca gtctcgattt atgtagcgat
    52441 atcaatcagc ttaatgaata tctcccgctt tatgccttca tcaacaccca ctcgacgatg
    52501 gatgtcagcg tgcaggatat gcggatggcg ctctggtttt ttgaatatgc gctggggcag
    52561 gcggaagata tcgccattcg tatgcgtcag tacaccgacg aatatcttga taacattaca
    52621 ccgccgttca cgaaagcctt gtttacctac gtcaaagagc ggaagtacac cttttgtacg
    52681 ccgggtcata tgggcggcac cgcatatcaa aaaagcccgg ttggctgtct gttttatgat
    52741 tttttcggcg ggaatactct taaggctgat gtctctattt cggttaccga gcttggttcg
    52801 ttgctcgacc acaccgggcc acacctggaa gcggaagagt acatcgcgcg gacttttggc
    52861 gcggaacaga gttatatcgt taccaacgga acatcgacat cgaacaaaat tgtgggtatg
    52921 tacgccgcgc catccggcag tacgctgtta attgaccgca attgtcataa atcgctggcg
    52981 catctgttga tgatgaacga tgtagtgcca gtctggctga aaccgacgcg taatgcgttg
    53041 gggattcttg gtgggatccc gcgccgtgaa tttactcgcg acagcatcga agagaaagtc
    53101 gctgctacca cgcaagcaca atggccggtt catgcggtga ttaccaactc cacctatgat
    53161 ggcttgctct acaacaccga ctggatcaaa cagacgctgg atgtcccgtc gattcacttc
    53221 gattctgcct gggtgccgta cacccatttt catccgatct accagggtaa aagtggtatg
    53281 agcggcgagc gtgttgcggg aaaagtgatc ttcgaaacgc aatcgaccca caaaatgctg
    53341 gcggcgttat cgcaggcttc gctgatccac attaaaggcg agtatgacga agaggcgttt
    53401 aacgaagcct ttatgatgca taccaccacc tcgcccagtt atcccattgt tgcttcggtt
    53461 gagacggcag cggcgatgct gcgtggtaat ccgggcaaac ggctgattaa ccgttcagta
    53521 gaacgagcgc tgcattttcg caaagaggtc cagcggctgc gggaagagtc tgacggctgg
    53581 tttttcgata tctggcaacc gccgcaggtg gatgaagccg aatgctggcc cgttgcgcca
    53641 ggcgaacgat ggcacggctt taacgatgcg gatgccgatc atatgtttct cgatccggtt
    53701 aaagtcacta ttctgacacc ggggatggac gagcagggca atatgagcga ggaggggatc
    53761 ccggcggcgc tggtggcaaa attcctcgac gaacgtggga tcgtagtaga gaaaaccggc
    53821 ccgtataacc tgctgtttct ttttagtatt ggcatcgata aaaccaaggc aatgggatta
    53881 ttgcgtgggt tgacggaatt caaacgctct tacgatctca acctgcggat caaaaatatg
    53941 ctacccgatc tctatgctga agatcccgat ttctaccgca atatgcgtat tcaggatacg
    54001 gctacacgtg tatatagtat tgatttatat gtgtaataac gatgtttata gtaaccgatt
    54061 ggatgttgtg ggggactgat gggggtgtat ccatatttta cccgcaaggt gatgatgaac
    54121 gtgtacgcaa taaatcacgt acacactgaa ttacatatac gtgtatatat aagtcttatt
    54181 gctacggagt tgtctcgttg actcagatca caacatattg atttttaaac atatttcccg
    54241 agcaatttga gacaactaca attctgcctg atgtaactca ttgatttaca aagtaaactt
    54301 ctggtaaaag ttgtctcggt caggttattg atttataagg atttattttt atctcattga
    54361 tttatagtgt gttttttctt gtctcactcc ggcgctggtg gcggttttcc gggcttccgg
    54421 tggtgatgcc ataaaacgct ctcagtcgcg ctctgacgca atatttgtaa aaggtactgg
    54481 ttggtatgcc tcagggtatt ttatctcgct ggtagcgcgt ttatcggcct tcctgtcggt
    54541 gtctgaatgt gattacccac aatgctgttt taccctccac tgggtgctgg tgagtcgcct
    54601 ttatcgctgt gtgagtgaga caacatcttg gggggatgtg agcggcgaac tgatgaacgt
    54661 gtaccctgta cacgaacgga gaagatggat cgcatatgca acatagtact caattcgatt
    54721 cgctgggttg gccgcagttc gtcccgccag ataaaccatg tagacagtca atacatattt
    54781 ttaaacggag gcagaagcag tgtagcccct tcctggctgg tggaggggtg gcggtgctgg
    54841 tggggcgccc gtctcttcct ctgcgactgc gcgagcggcg gcgggggttc tggaccgtcc
    54901 ggcatccgcg cggccccgca ctccttaccg tagtacacgc gatcgattcg tgtttccgaa
    54961 aatttttttt gttctttccg ggaggggtgg ggggtgttgc ctgcctgctt cctgaggccg
    55021 tgcacagaat gcccggaaaa tttttttgtt ttttctgtgg cgggtatgca gtgaagagtt
    55081 gcaacaagtt acgggtgata ccgctcgccg caggtccgtg accgaacgcc agtgagcgaa
    55141 cggatgagga agcggaaagg cgaaggcgtt acatcgggca cggacgcacc ggagtctggc
    55201 agcatggttt gccgaaatgt gtggcggtta tccttccgct ggtggttgtg cctgagcggg
    55261 atgacagaca ctaccccgct ggtgctgtgg tgctggctgg ttgtgttacg tgcgctggtg
    55321 taatgtgatg gggcgtcatg gaaacagatg ctgccgatgt ggtggctgcc cggaacagcc
    55381 ggaacgacta cgggcgtggc agttattcct ccgctggtgc tgtgcggtgt tctgctgccc
    55441 tggctggttg tcggcatggt ggcacatgtt acggctgtta tgatgtgcga tatggtttcc
    55501 gcgcgttatg ctgcgttgtg cgcacgttca ggcgcattaa cgtaacgcgt tactgaactg
    55561 gtgaacccgt ggcaaggggg tcgttcgtgg ctgcggcgaa ctgaaacatc ggcgggatgg
    55621 tggtggggct ttattgttct ggtctgcatg ttaccgggcg gggtgagtgt tttccccgct
    55681 ggtggttggt tcgcgtcatg cagctgcttc gcgtcgctgg tcatgcgtcc acgttcgggt
    55741 catacactga ccagtcaaac gaaggattct taagcgtgtt ggtggcgaac gcttcccgtg
    55801 cctgctcgag cgggtacatc ctggctacac gggtgttgcg gttcttcttc ccttccggtt
    55861 cgcgtcggta ggccagtgtg ccaatgccgg acgtggtgac ggcgtggctt aacaccgact
    55921 ggcccagctg gtcgaagtac tggcttttca tgaagtgcgt atacagtgat ttcgtgcgta
    55981 tccacacggg ccagccttcc gactttaatc cggtataccc ccgcttgagg cagtggtaca
    56041 ggaagcgttc cgcgggtctg aggtcggagg gcagtaccac gggcggcgct gctgtgtaag
    56101 ccacgacgcg cacatcgtgt aactcccgct ggtggctgta cagggggatg agtgaggcgg
    56161 ctatagcaag ctcttcacgt gagcacttaa agaacgcttt gaggtcagcc tcatacataa
    56221 cggcatcggg gccgcgtccg gtttctttga tccacctgca aatcttttcc gccagctttt
    56281 ccgagttgag gcggttcttc tctttccgct ggcgctgtgt gtctgccccg gtccgttcgt
    56341 gtacgtgttg aaggaacgca cgccccggca gttgtgacac gggttccggt tcgttgagga
    56401 tgcggtttaa cagggtgtcg ctggtatcgt tctgcatgtt gcagcctccg ttgttgcgcg
    56461 aataacttgt tttatataca catgtatatt aatacagtat ggcgtggtac ggctattgcg
    56521 ggggtggggg tactggctgt atagtgattt acaggcattg ggatagcaac ggatgtcagg
    56581 atggtataag ctgtgcggtg agtctggtat tggtttattc cgtaacttac tccccgccgc
    56641 ggcttccgtc ctatcgtctg cggcgttttg cccgtcagat ttgtcacctg acgggctttt
    56701 ctttatgggc gcttacccgt agtcaggccg ccagtcctgt cctccgttcg cgcggtgcat
    56761 aatctcgcgc agccggataa caagcgggtc gttatcgatg tctgtatcgg acttagcggg
    56821 agggagtgga cgaagcccct taatctcttc cgcctgttcg tcggtgacgc tgcgccgttc
    56881 gccgttactg agtatgacct caagcgcgtg aataacagta acgcagcgcc gccgcttcgc
    56941 tgtgtctgcc cacaccacca gcatttccac accagcgaca ccaaacacat cgccggggcg
    57001 aagagggtat ataacaggca caaggtcgcc ggataccagg acgtgttcag ttcccctgtc
    57061 ggtcgcgtgg tactcgcacc ccttcgtata gtcgcgcacc ttgtacctga accggacggg
    57121 gactaacacc ccgccggatt gtgggttatg cctcggcggt ttcgccacga cacagacagc
    57181 tttgatctcc ctgctcatag cccctcctgt agactctccg cacgggccat cttatagctt
    57241 tcctcatagc accactcatt tgtgtaaagt atttcgacac tcatccgcgg gcggcggccg
    57301 cgtcgtcgcg catcgtaatg tcgtaggaca agctgcgcca tacactcctc ctcttcagca
    57361 tggggcagcc tcccatccgt aaacatcgga cacaggtaca taacatcctc aaatataaac
    57421 accatatccc cccgctcgag gaagtccata tatgtgccct ttgcggggag cgtgtggtat
    57481 atgaggtgcg agccgctcgg ggctacgtgt accatcggca cgcagaaggc gccagtctca
    57541 gcgaacagat gcggacggtc ttctataatt gccgcagtca ccagcgtatc cggccagtta
    57601 aatatcttaa tactactcat tggcttactc cccctcgact gcatccagta gtttcacttt
    57661 atagaaccgc tgctgactcc cgccgatacg cagccgggat tgtcgggact ctgaacctgt
    57721 ttcgcgttcc agcatccccg ccttctctag tgcggcggcg gcctgcctgt agttgtggcc
    57781 ctttgcgatg tggttcctga acgcagcggg agccacgtag aaccactcat cgccgttatc
    57841 accatcggtg ggcttccggt atccgcatag tctgcccatc cggctggcct cctggtctgt
    57901 cgcggcgttt gccttcccgt cagttgtgag cgtggcgaac tggctgcgcc cgtactgcat
    57961 gaggaagtct gtcgcctggc tgataatctg actctgtgcc ttgtcacgcg taccgaagtt
    58021 cttcacccac gacttgaaca cattcaccac tgcatcacgg cacgcggcct cattccagcc
    58081 ggtgaacggt cgcgccataa caagcgccgc atccagtaca gcgaacgcat cagccacacg
    58141 gcagacctgc gggctgtcag ggaagcgctt ctggaccgct gcctgccact catccaggcg
    58201 ctgacggata gtgtttcctg cctccggcag gttccgtatc agccaggtaa tccactcccg
    58261 gaatactgcg ccgaagttgt cgttgcacgc acgggtaagc gcgcgggcgt gtgctccggc
    58321 tgtgtggtat tcgtgaaggt tttccggctg catctcctcc accgggatat taagcaagcg
    58381 gacaagctgc cctggtgcaa gcgtgatttg tgcctgacta gcgaaggctg ccgtatcggc
    58441 ctcgccggta ctgaggaccg ggagccgcca tgaatcatgt ggcgcgttgc tgccgtcttt
    58501 cgttgccctc acgcgtcccg aaccgttaaa cagggagtag attacgctgg caagggcttt
    58561 cgggcttcct tcgcccagct catcaagcca gagaaaacca tcacaacggg cagccgcttt
    58621 gttctgtatc ccgttcgctg ttccgttcca tgtcattttc agcacagaag gttcaccgca
    58681 tacactcgcc gcgagtgtcg cgcatgtggt cttacccgtt gttgaggggg cgtacaggtg
    58741 gaggccaaag ttctgtatac ctgccagctt cagcaacggc ccggcgaagg gtatcgcaac
    58801 acataccatc atgaggctgt taccatccac cagccgggcg actgactccc gccaggtatc
    58861 agccgttcct cttgcggtcc agccgccggc tacgtcgctt ttatccatga acagcgccgg
    58921 gatttcgtca ccaataacct gaccatccgg cagaagatac gcgcggttgt cgtcggtcca
    58981 gccagcggac gctgccagcg tccagcgcgt cgcgggtttc tgtccgataa tccagttcgc
    59041 cagcgctgac cgtagctgcc tgccatcgcg caccagtagc ccgccatcca ttaacgtggt
    59101 aagcgctgcg ctggaggcga agccgcccag gtttaccgcg acggtggcgg ggcggccgtt
    59161 gttcatccac cgaagtaccc ggtactcttt gccctcatag ctaccgtagc ccaccacgtc
    59221 caccttctgg cagatatcca gcgacgtgtc acggaaacga aggcgcatca tcggcccctc
    59281 gtcagtgtcc tcaatcacga caccagattc gccgtcgctg gtggctctgc gctgtttacg
    59341 gctggtggtc ttctctgccg ctgtcggtcg gtacatgctc ccggcaaaca gcatagtagc
    59401 cttctcgcgc ccttcgctga caagtacatc attccagtcg cggccctccc cgtcctccgc
    59461 cggggccagt gcgaccgtac cgccgacggc aagggcggct ttttcagcgg ctaccttccc
    59521 ggtgtttttg cccttcccgg tgttatcgtt atcggcggcg atgataatct ctgcttccgg
    59581 gtggcgttcc cgcatggttc tggcgacggc ttccaggttg cccgctgagc ctgccatcac
    59641 ggccatcgcg tcgtgctgta gctggtgggc acttaaggtt gtggccagac cttcggcgat
    59701 gatgatacgc accggctgcg tctgctggtt gatggtgtgg aaagcgccct ttgctacacc
    59761 tccgaagcgc ttttctttac cgtccgctga tatcgtctgg aatgccacca ccacgtcgct
    59821 ggcatcctgc atcgggatga gggttcgccc accactcaaa accaggggct tcgttgcgaa
    59881 actctccagg cctttacgct tcaggtacgg gcttgccgcc cccgcgacgg tgtcacgcag
    59941 gaagtcctca agctcagccc tgataccgtc ggcaatgacg ggcgtcgctg ctgcccctgt
    60001 ccgggctggc gttcctgtgg tgtgcccggc ggtcacagta tcggtggatg acccggcagc
    60061 gcccgttaca gttgtgctat catgtacgtg ttgaggctgc atcatattgc cccctgcgtt
    60121 ggtgtggctt cgacaatacc cagcacatta cccaccagct ttgccgcctg gtatgtgctg
    60181 accggggagc cgaaccgcac ggatgcgaca cgtctcacca gttcaagccc gtcgcctgcc
    60241 tggtagtaag cgtcggtttc gtccggacac ttgcggcaaa caaacgttcc tcgcccctcc
    60301 ttatcgtcga aggtgaaacg gtcatcacca ccgcaggcgg ggcatggtcc accctgacca
    60361 actggcggaa cgtcaatcac aagccgccgc agaataaggg gccattgtcc ctcagctgct
    60421 tcgacaacgg cgcgaatata tcggcctgcg cgatttgttt ttaaagtcat aataaaatcc
    60481 tttccgggga atcccggtgt ttgtgtttaa caaagtcatg acggctatcc tgtgtggtat
    60541 ccattctaat cggcggtaaa taattgttca gttgttgcgt cataatatta tggtggttat
    60601 gcctgcataa tattatggtg gttatgcctg cgtaatatta tggtggtcgt gcaccgaatt
    60661 agtgacaacc gtgtaattca atcccgttgg taattgctgg cgcggaacta catcgcatca
    60721 agtatctctt tagcgcggaa taacagttct tcggtactgg ttaacgtcac gtagtcgctg
    60781 tcttcatcat cgccagtatc cggcccgtgg tccgcacgtg gtcggctgcg tgcccactcc
    60841 accagttccg acaataacca cgcgttcttc gcggtcttcg tgtcgtccag cctgcgcggt
    60901 acggggaaag tgccggcttt ttccatacgc aaacgcgtca tccggcatag cccggtatat
    60961 gccttacatt ctttttcgta taccagtcgg tcaaatataa cggctcccgg tggtattgtt
    61021 aaaaggtcgg tgcgttgttt gtctgtcata ttaaatctct ccgctgctgt aggtttatat
    61081 aagtgatatg cggaaattaa tatggcatag cgcaataatc gaatatatga tttaacccat
    61141 gaacattata agatcaaaag atcaaataag atcaaaataa gttcattata aatatgaggg
    61201 cgttataaaa ataaccgcct gtaaaagttc aggcggatta ataaaaataa agttagttag
    61261 tctttcttga tggggtgtaa atgggcctct gcccggcgtc tgatggaggg acgcgcggcg
    61321 gtcttttctg cgtcattccc tatatgcttc ccgccgtatt tgccgtatct gttactggtt
    61381 tttcttgcgc gatcccccac atcggcatca tacacggcag ctacgccgtg aagcttcgca
    61441 aggcatagcc atatcatccg cgtaaggtgc tcaggcgttt cgccgcctct cgtacagtct
    61501 ggccatgagt cagtacttac cccggctatt tcttccacgc gctggcgctc accatcagaa
    61561 cgtttctcaa gccacgcatt aatcacctcc tggaattcgc caccgggccg catcttctcc
    61621 agtcggacgg gtaaatcatc catagcattt actggctggt ggataacatg ccccaccagc
    61681 accgcaaggg cacggcggcc tacagcatct tccctggcgt gtaacacgcc gggatcaaag
    61741 tcgtctgttg tgtacggggt attctgcgcg gcggtatctg ttttcgctgg tgtagtgttt
    61801 gccttcgctg gtggctggtc tactgctgtg gtgttcgcgt catgcagctg cttcgcgtcg
    61861 ctggtggaac tggcgagaag ttccttaagg atatgaaggc cgtcatctcg tatggtcatg
    61921 ctgttcgtca gtgcttccgg cagtgggcga gtaacctgcc agtacaatgg cattacgccc
    61981 ggttgctgcc agcgccatgg ggcaagtcgg aacgtgacaa ggtcatccgt gagcactgct
    62041 ccccatatgc ggaacatacc accggcaaac agccgaatac ggtgcggagg ggtatcaggc
    62101 gagtcgggtt ctgcgccgta atcgtaagtt tcctcatcac cccacaggac gcgcctgtat
    62161 gcgaaggggg aaacagggac tgatatgtgg cacgcactcc ggtaatcttc gcgcgtcacc
    62221 cacatacgaa gccggagagg cccgacttcc tcgctgatat agcggtcgct gtcaccatca
    62281 agcgacaaag gaacgccatc gcggcggtat tcatgcccgt acatgccggg gctgtaaata
    62341 tcaacaaacg ccccggtgtc tgtccactgt ggcacacggg caatcagcga gagtaaatca
    62401 tcatcggagg aaaggttaag gcgatcccgc acctgtcgga atgataaggc ggtcatgttg
    62461 ttgcgtcctg tcgttgctgt tccccggtcc ggtcatgggc cagaccaggg aggtggtaac
    62521 ttacatgctt attaattttt tgttttctgt aagggtatta cattatcgcc ggatgcgagc
    62581 gcgtcaagcc actctaccca cttgttaagc gcttccgttt tttccgccat gtactcacta
    62641 cggttatata ccgccataac gccctgcatg acgtggccca acatctgttc caccacataa
    62701 agcgcggtgt gtatatcctc attgtgtcgg gttgagaacg ttcgccgcag gtcatggaat
    62761 gaccagcgtt taaggtgtcg gaaccgtgcc atcttccagg cgcgattgcc ccatgatcct
    62821 actgtctttt ctttcttctc cacgccgagc agtagcccgg tctttttgtt cgcctgtatc
    62881 agtcgggtaa tcactggctt tatggcatcg ggaacggggc gacgaaccgg aatgccgcct
    62941 ttggcgttgt gctctctggg aaccgtccat atccatttat ccacatccca ctcgtcaatc
    63001 cgtgacagtc tggcttctac cgttctgcaa ccaaacacga ttaacaaccg catcaaatcg
    63061 gcatagtagg ggtttaagct gccatcgctg gcggccttcc atatgtcgat aagctcgtcg
    63121 ttatccagta cgcgggcgca cttcccggag ggtacaccgc cgaccgcttt gacggtcatt
    63181 ttgtccagcg cgttactggt tgcgtacccg aacgcatcgc aatagttcag cgcctgctta
    63241 cactcaccga gggccttacc tgccgtatag ggatacttgt cggctatctt gcggaagcag
    63301 tcacgccagt ctcccgcgct gcactcatcc agcggacgcg aaccgatgga actgatgatg
    63361 tatgtattca tccggcttat aaccgtgccc ggtcgtttcc acgtgttgcg gacgtagtta
    63421 tcaagccagt aggtaatggc atccgctacg gtgatacgct tcacaggttc cgcgctggct
    63481 gcgcgtcggc gctcgtattc tgtcttcggg tctttccctc gcgccagcca ttcccgacac
    63541 tcctgccgta actctcgcgc gaccttaagc cccacatccg ggtaacggcc aaaatacact
    63601 ttaacggctc tggcctcgct ccctttctgt ttgtactgga agtaccaggt gataaagccg
    63661 cctttctgac acctggcata gagtccaccg ccgtccgata atgtttcttc tttgtcgcgt
    63721 tcaacgccgt tacgtcgttt cagttcgatg tctttgagtc tgttaattgc cccgcccata
    63781 ctccccccgt ttttacctgg ctggtgtgaa tttacctgtt ctgtcttgtc tctcttgtct
    63841 cattcagcgg gtttcttgtc tcagataacc agcgagggat tgtatatatt tattatattt
    63901 atcatataga tacatatata tatataaagt gagtgtgaca agtaggacaa gtgagacacg
    63961 tatatttata tacacatgtg agaaaacaca ttttgcttct tcgctagcta tatccccccg
    64021 tatccatata cccgctgttc ccgtccgtgg ggtactttgg ggatccgtta catatacgca
    64081 gtatcctaaa atccttccgt tccatctctg tggggtactc tgtgggatcc taattcgctt
    64141 tttaaatata cacaagtacc gcataaaaat ttacagcaac atgcgcatta cgcattaaat
    64201 atatagtaat caattggtta tgtgattgag ggtttacaga gatagacgag aatgtaaggt
    64261 aagcgtatcc gtattcagga tctggcgcaa gggatccata agctgattcg taaacacgat
    64321 ctttccggtt tgatgttgcg ggcatttgat accttgccgg agatgatcat gacgccacat
    64381 caggcatggc agagacagat taaaggcgaa gtggaaacca ttgcgctgga acaactggtc
    64441 ggtagagtat cggcaaatat gatcctgcct tatccacctg gcgtaccgct gctgatgcca
    64501 ggagaaatgc tgaccaaaga gagccgcacg gtactcgatt ttctactgat gctttgttcc
    64561 gtagggcaac attaccccgg ttttgaaacg gatattcacg gcgcgaaaca ggacgaagac
    64621 ggcgtttacc gcgtacgagt cctaaaaatg gcgggataac ttgccagagc ggcttccggg
    64681 cgagtaacgt gctgttaaca aataaaggag acgttatgct gggtttaaaa caggttcacc
    64741 atattgcgat tattgcgacg gattatgcgg tgagcaaagc gttttactgc gatattcttg
    64801 gtttcacgct gcaaagcgaa gtctatcgcg aagcgcgcga ctcgtggaaa ggggatttgg
    64861 cgcttaatgg gcaatatgtg attgagcttt tctcatttcc gttcccgccg gagcgcccca
    64921 gccgaccgga agcttgcggc ctgcgtcatc tggcttttag cgttgatgat atcgatgcgg
    64981 cagtggcgca ccttgaaagc cataacgtga agtgtgaagc catccgtgtc gatccataca
    65041 cgcaaaaacg cttcacgttc tttaacgatc cggacgggct gccgttggaa ctgtatgagc
    65101 agtaaggctt gtcatcgccg catttgcccg gtaacgtgcc gggcattgct actgtaaaat
    65161 cgcatcatca tgacactcac gctcaataga caacttctct cctcacgcca gattctggtg
    65221 gcctttagcg gcgggcttga ctccaccgtt ctgctgcatc agttggtgca gtggcggatg
    65281 gaaaatccgg gcgtcactct gcgcgctatc catgtgcatc acggtttaag tgctaatgcc
    65341 gatgcctggg ttaggcattg cgagaacatc tgccaacagt ggcaggtgcc gctggtggtc
    65401 gagcgcgtgc aacttgcgca agaaggactg ggcattgagg cccaggcgcg gcaggcacgt
    65461 tatcaggcat ttgcccgcac cttgttaccc ggtgaagtgc tggtcaccgc gcaacatctc
    65521 gacgatcaat gtgaaacctt tctgctggcg ctaaaacgcg gtagcggccc tgccgggctt
    65581 tcggctatgg gggaagtttc ggagtttgcc ggaacacggc ttatccgccc gttgctcgcc
    65641 cgcacgcggg gagaactggt gcagtgggcg cgtcagtatg atttacgctg gattgaagac
    65701 gaaagtaatc aggatgatag ctacgatcgt aacttcctgc gcctgcgcgt ggtgccgtta
    65761 ttgcagcagc gttggccgca ttttgccgaa gcaacggccc gcagcgctgc gctttgcgct
    65821 gaacaagaga gcctgttgga tgaactgctg gcggatgata tagcacactg tcaaacgtcg
    65881 caggggacgc tgcagattgc gccaatgctg gcgatgagtg atgcccgccg cgcggcgatt
    65941 atccgccgct ggctggcagg gaagaatgca ccgatgcctt cccgcgacgc gttggtgagg
    66001 atctggcagg aagtggcgct ggcgcgggaa gatgcttccc cctgtttacg cctgggcgcg
    66061 tttgaaatcc gccgctatca gtcgcaactg tggtggatta aatccgtcac tgggcaaagc
    66121 gaaaccattg tgccgtggca gacgtggcta caaccgctgg aattaccggc gggactggga
    66181 agtgtacagc ttactgcggg aggcgatatt cgccctccgc gtgcagacga agcggtcagc
    66241 gtgcgtttca aagcgccagg attgctgcat attatcgggc gcaacggcgg acgtaagctg
    66301 aagaaaatct ggcaagagct gggtgtgccg ccgtggctac gtgacaccac gccactgctg
    66361 ttttatggcg aaacgttgat tgcggcggca ggggtatttg tgacgcaaga aggtgtggct
    66421 gaaggtgaga atggcgtcag ttttgtctgg cagaaaacgc ttagttaagt gaaagccgga
    66481 taagacgctt caaacgtcgc atccggcgaa agtcaatcag gactcgctca ccaccaccgt
    66541 accgatttcc gggtggctaa agctggtaat tttatccaga cgcagctcgc gggttgtacc
    66601 agcggcctcg acgaccagat actccacatt tttgcgggag actaaatcgc tggccttcgc
    66661 ctgcaatttt tcgccatctt tcagctcaag tgtcagcatt aaatgatgct ggcaggcgag
    66721 ctcaagatta tcgtaatcat cacaattgat tggttgatac gtatcattca ttgacataat
    66781 cgctcaccag taagtttgcc gcagcgtatg ctgctttttc cctgacagcc tcagaaaggg
    66841 cgtcgtcggc agccatttca ttcagcactt tcaaaacgca gcccagcgcg tccggaacgt
    66901 atcctaagtc tccgctggcg atttccgcgt accgcttgcg tattaactca caatattttt
    66961 ccacatgccc tcctgtcagc actctgactt aaccgtggat gcaagtctaa gcctacgaag
    67021 ataaactctg tttcgcaagg tgactatacc acactcattt ctgcaatatc agcgccgcaa
    67081 ctgcacgtat tccgttacaa tggcctcctg attcgaaagg agttttctta tggcgcttaa
    67141 agcgacaatt tataaagcga cggttaatgt ggccgatctc gaccgcaacc agtttctcga
    67201 tgcctctctg acgctggcgc gccacccttc agaaacccag gagcgcatga tgctgcgcct
    67261 gctggcgtgg ctaaaatatg ccgatgaacg tctgcaattt acccgtggtt tgtgtgccga
    67321 tgatgagccg gaagcgtggc tgcgtaacga tcatttgggt attgatttgt ggattgagct
    67381 ggggctgccg gatgagcgac ggattaagaa agcctgcacc caggccgcag aagtggcgct
    67441 gtttgcctat aatagtcggg cggcgcaaat ctggtggcag caaaatcaga gcaaatgcgc
    67501 gcagttttcc aatctttcca tctggtatct ggacgatgaa caactggcga aagtgagcgc
    67561 ctttgccgat cgtaccatga cgctgcaggc aacgattcag gatggcgtga tctggttatc
    67621 ggatgataag aataatctgg aagtgaactt aaccgcctgg caacaacctt catgattgtg
    67681 atttcccgac atgttgctat ccccgatggt gagcttgaga tcaccgccat tcgtgcgcag
    67741 ggcgcgggcg ggcagcatgt taataagacc tcaacggcta ttcatctgcg ttttgacatt
    67801 cgggcgtcca gcctgccaga gtattacaaa gaacgtctgc tcgccgccag ccatcacttg
    67861 atcagcagtg atggcgtgat tgtcattaag gcacaggaat accgcagtca ggaactgaac
    67921 cgcgaagcgg ctctggcccg gctggtggca gtgattaaag atttaacaac agaacaaaaa
    67981 gcccgacgac ccacgcgccc cacccgtgca tcgaaagagc gcaggctggc atcgaaagca
    68041 caaaaatcaa gcgtgaaggc gatgcgcggc aaagtgcgca gcggtcggga ataaaaagaa
    68101 ggaatggatg gtgaaaaaag cgatagtgac agcgatggct gtaatcagcc tctttactct
    68161 gatgggttgt aataatcggg ccgaagtcga tacgctttct ccggcgcagg ctgccgaact
    68221 gaaaccgatg ccgcaaagtt ggcgcggcgt gctgccgtgt gctgattgcg aaggaatcga
    68281 aacctctctg ttcctcgaaa aagacggaac atgggtgatg aatgagcgtt atctcggtgc
    68341 tcgtgaagaa ccttcctcct tcgcttccta cggtacatgg gcgcgaaccg ctgacaagct
    68401 agtattaacc gacagcaaag gtgaaaagtc atattatcgg gcgaaaggcg atgcgctgga
    68461 gatgctcgat cgtgaaggca atccgattga atcgcagttc aactatacgc tagaaccggc
    68521 acaatccagt ttacctatga cgccgatgac cctgcggggc atgtattttt atatggctga
    68581 tgcggcgacc ttcactgatt gcgcgaccgg aaaacgtttc atggtagcga ataacgcaga
    68641 gctggagcgt agctacctgg ctgcgcgcgg tcacagtgaa aaaccggtgt tattgtcagt
    68701 agaaggtcac tttacgcttg agggtaatcc ggataccggt gcgccgacta aagtattggc
    68761 acccgatacg gcgggtaaat tttatccgaa ccaggattgc agtagtttgg ggcagtaatt
    68821 gaaagccgga gcattctccg gcttttatca ctgagtcagc ccaacaaagc gacccgcctt
    68881 gatataaatc cctggcttca tgtgcccaac atactgtaac tgcgtttctg gcttaaacgc
    68941 agagatatcg ccagtacgca tatgcatcag atcggcaggg ctaatccagc ctgattgcgc
    69001 cagcgtaagc ggatgcccgg ctttggcaaa tgcatcagtg acaaattccg aacaaaacca
    69061 cgactttttg tctccttcgc ccacactgct taactgcgct ttcgccaggc cgctgacgca
    69121 ctgttgacga aaatcctcgg agaacggatt cagtgagcac acctggcgag tcaccataaa
    69181 gggaataaat tcgacaatgc cgcgatagtt ataaccgcta tctttgattt tattagcaaa
    69241 cgcggtgata tctgtggctt gttgcggggt aagatccggg actcgtaaga cgaaaagctt
    69301 atcactgtgc ttcatcgctt ttttaaggga aacgatctgg acgccagcgc ctgtcgcttc
    69361 tgcaacgtta ttatcaccca gaaagattgc aacgtgactc acagaggaag tgctgaagac
    69421 gcggattcca aatgaggtta ccccaaggct tgaggagaac agcaaatcgc cgggtttgag
    69481 atcaggtgcc gttatttctt ttattgattg ttcggtgaaa gagctttgat gctggaattt
    69541 gactacccat gtttttgcct cgacatctac cgctgtggca gaggagtctg gctggctgat
    69601 atcaacggta caggccgaaa ggaaaagaaa gcaggggaga agcaggcggc aatgcgcctt
    69661 tggtttatcc attttataca atccatgtaa aaaagggccc tgaaattcag gaccctttct
    69721 ggcatcagcc tttaatctgt ttcaccagat attcgacgat gtcaccagtc ttaattaact
    69781 gtttctcgcc gttacgacga tatttatatt cgatatcatc gttgtcgagg ttacggtcgc
    69841 ccagcacaat agtgtgcgga ataccgatca gttccatatc agcaaacatc acgcccggac
    69901 gctctttgcg gtcatccagc agcacttcga taccttgtgc acgcagttcg ctgtacagtt
    69961 tctcagcaag ctcttgtacg cggaaggatt tgtgcatgtt catcggcaga atcgccacct
    70021 ggaaaggcgc gatagcgtca ggccatacga tgccgcgttc gtcatagttc tgctcaattg
    70081 ccgcagccac aacgcgcgtt accccgatac cgtagcaacc catcgtcagg atttgattac
    70141 ggccatcttc accctgtacg gaggctttca gtgcttcgga gtacttggta cccagctgga
    70201 agatgtgacc aacttcgata ccacgtttga tcagcagcgt accctgacca tccgggcttg
    70261 gatcgccagc caccacgttg cggatatcag caatttccgg ggtagcgaca tcgcgatccc
    70321 agttgatacc gaagtagtgt ttaccatcga tgttagcacc agcagcgaaa tcactcatcg
    70381 ccgcaacggt acggtcaatc accaccggaa ttggcatgtt taccggaccc agtgagccag
    70441 ggccggcttt aaccacggca cgaatttctt cttcggtcgc gaaagtcagc gggcttgcaa
    70501 cctgcggcag tttttctgct ttaacttcgt tcagctcgtg gtcaccgcgc accagcagcg
    70561 caaccagcgg gaagctactg ccttcaaccg ctttaaccag cagggtctta accgttttct
    70621 caatcggcag attgaactgt tcaaccagtt ccgcgatggt cttcgcgttc ggcgtatcaa
    70681 ccagcgtcat ttcctgggta gcagcagcgc gcggttcttt cggcgcgata gcttctgcca
    70741 gttcaatgtt cgctgcatag tcagaggtgt cggagaagac cacatcgtct tcaccgcttt
    70801 gcgccagcac ctggaattcg tgagaggcgc taccgccgat agaaccggta tcggcctgta
    70861 cggcgcggaa atccagcccc atgcggctga agattttgct gtaggccgca tacattgcat
    70921 cgtaggtttc ctgcagggat tcctgagagg tatggaaaga gtaagcatct ttcatcagga
    70981 attcgcggga acgcatgacg ccgaaacgcg ggcgcacttc atcacggaac ttagtctgga
    71041 tctgatagaa gttcagcggc agctgtttgt aagagctcag ctcgttacga atcaggtcgg
    71101 taatcacttc ttcatgagtt gggccaagta cgaacggacg gtcaccacgg tcagcaatac
    71161 gcagcaattc cgggccatac tgttcccaac gaccactctc ttgccacagt tcagatggct
    71221 gaaccaccgg cattaacacc tcaatcgcac cggcgttgtt catctcttca cgcacgatgt
    71281 tttcgacttt tttcagaacg cgcacgccgg tcggcagcca ggtgtataac ccggaggcca
    71341 gcttgcggat catcccggcg cgcagcatca gctgatggct gatcacctcg gcgtcggcag
    71401 gtgtctcctt gagagtggag agcaggtatt ggctagtacg catgttgtta cggttccagt
    71461 tggaaggtag aacaggctca aggtgagcct gggacaaaaa aagtgattta gtttaccagt
    71521 gcaaaagaaa tgtcaaaaga gaagggcgtg aatttaacgc ggttccagcg caaagacttc
    71581 aaaacctgcg tcggtgacgc gccagcgaac gttaaaatca tgcagccaga cggcataggt
    71641 tttgcccgtt tcctcacctt tacgataggc cgggcgcggg tcctgcgcta gtacttcgcg
    71701 gataaacagc gttaactgcg gataacgctt ctccagcgtc aaaagctgct tttcgacctc
    71761 tgcggtaaaa ctcaccgcca tctctgcagc tggcgcactt tgcgcatagc tggcactggc
    71821 atttggaagc gattcggcaa aggggagata cggtttgata tccactaccg gcgtaccatc
    71881 gaccagatcc agactgccga gcttcagaat cacgctgtct ttatggcaaa caacctcttt
    71941 cagctctacc agcgacatac caattgggtt agggcgaaaa gtggagcgtg ttgcgaaaac
    72001 ccccattctg gcattaccgc caagacgagg cggacgcaca gtcggacgcc agccgccttc
    72061 catcgtttga tgaaagacga aaagtatcca taaatggctg aacgcttcca ggccgcgaac
    72121 ggcgtcggct tggttgtagg gagcaatgag atgcagttcg ccgttggcgc tttttaccag
    72181 acctggttgg cgcggaacgg cgaatttttc tttatagggc gagcgaataa cgcctatttg
    72241 ctcgaactgg aaactgctca tttcgccgta atgttaagcg cagaaccgat acatacagcc
    72301 tgacgatagc agcctggcgt accgctggtg acttcgcagc tatgcagtaa taccgcattg
    72361 gctttcattt tcgaggcgtt gatttgcatc cgcttacgcg cggttggaat gctcggcgga
    72421 gagtcctgat tagaggcttg gcaagagtcg ccactgactt caccgagatc gcggaacggt
    72481 ttgccgacta attcttctgc attggtataa attcggaccg gcgtggcgcg cggcactttc
    72541 ggttttgcag actccgcttt cggctggggt gcagtgcttt gaacgggttc gacaggggat
    72601 ctgcttaaca tggaacagcc gcttagcatg agtgctacta aacagatcgg taaagcacgc
    72661 atagtatttc ctcaatgtat gatcaaaacg tcaatattga atcaggagct tgtaaaaatg
    72721 acaagacggg caagcgcccg tcctgaatga tattacaaat tgtggaaaca gcctaaaaat
    72781 taccagcctt taacagcgcc gccgttaaac actttgtttg ctgcttcgta aacttcgtca
    72841 gactgataag cctggacgaa tttcttcacg ttctcggcgt ctttgttatc ttcacgcgtt
    72901 acgatcaggt ttacgtacgg ggactcttta tcttcgacaa agataccgtc tttcgccgga
    72961 gtcaggccaa tctggctggc ataggtggta ttgataactg ccagagcgat ttgcgcgtcg
    73021 tccagagaac gcggcagctg cggtgcttcc agctcaacaa ttttcagatt tttggggttc
    73081 tcaacaacat caagaacggt cggcagcagg ccaacgccat ctttcagttt gatcaagccc
    73141 actttttgca gcagcagcag tgaacgacca aggttagttg ggtcgtttgg cacggcaacc
    73201 tgcgaaccat cctgcagttc atccagtgat ttgattttct tggagtaacc tgcaatcgga
    73261 taaacaaaag tgttgcctac tgcgaccagt ttgtagccac gatctttcag ttgctgatca
    73321 aggtacggtt tatgctggaa ggcgttggcg tcgatatcgc ctttgctcaa tgcttcgttt
    73381 ggcagaacat agtcgttgaa tgttaccagc tcaacgtcca ggccatattt gtctttcgca
    73441 actttctgcg cgacttctgc aacctgctgt tcggcaccaa caatcacgcc gactttaatg
    73501 tggtttggat ctttttcatc ctgaccgcag cctaccagtg ccagtgatcc aatcagggct
    73561 cccactgccg caaaggtttt gaatttgaac gccatacctt attccttctt caattattta
    73621 tgttgtgttg aacgttactt gcgagtgaca gcccggacga tgcggtcgcc tgcgaactga
    73681 attaaataaa ccagaatgac cagcaatacc agtaccgtat tcatcaccgt cgcgttgtag
    73741 ccgatgtagc catactgata gccaatctga cctaaaccac cggcaccgac tgcaccaccc
    73801 atcgcggaat aaccgaccag ggtaatcagg gtgatagttg ccgcatttac cagacccggc
    73861 aacgcttccg gtaacagcac tttacggacg atctgcattg gcgtggcacc cattgcgcgg
    73921 gaagcttcaa ttaacccggt tgggatctcc agcagagcgt tctcgaccat acgggcaata
    73981 aacggtgctg caccaacggt taacggaaca atcgctgcct gcaaaccaat cgatgtacca
    74041 acaataacgc gggtaaacgg aatcatccat accagcaaga taatgaacgg gatggaacgg
    74101 aaaatgttca caatcgcaga aacggtacga tacagcttcg cgttagcaat aatttgcccc
    74161 ggacgcgtga cataaagcag gacgccaacc ggcagaccaa tcacaaatcc aaaaaaaccg
    74221 gatacgaagg tcattgccag cgtttcccat acgccacgaa ccagcagcca catcatcggc
    74281 tcagacataa cccagtacct ctacttttac atggtgttcc tgcagccagg taatggcggc
    74341 ttgcgtatct tgttgtgtgc cgtgcatttc agtcagcatg atgccgaact taacgccacc
    74401 ggcgtaatcc atctgcgcgc taataatgtt gttgttgacg ttgaaacgac gcgcggtttc
    74461 agaaagcagt ggggcatcga ccgattgacc ggtaaactcc agacgcaaca tcggcacgca
    74521 gtcagtgaat ggctccgctt gcagacgttc ctggtaatct tccgggatat ccagatgcag
    74581 ggtcgactga ataaacttct gcgccagcgg cgttttcgga tgcgagaaca cttcacttac
    74641 cgtgtcctgc tcgatcagtt cgccattgct gatgaccgcc acgcaatcac aaatacgctt
    74701 cacaacgtcc atttcgtggg tgatcaacag aatcgtcaaa cccagacggc ggttgatgtc
    74761 tttcagcagt tcgagaatag aacgtgtcgt tgccggatcc agcgcgctgg tggcctcatc
    74821 acacagcaat actttgggat tgctggctaa cgcacgggca atcgccacac gctgtttctg
    74881 cccaccggaa agattcgacg ggtagctatc atgcttatcg ccaagaccaa ccaatgacag
    74941 caattccgtc acgcgacgtt tgatctcatc tttcggtgtg ttgtccagct ccagcggcag
    75001 agccacgttg ccaaaaacag tacgcgaaga gagcaggtta aaatgctgga aaatcatacc
    75061 aatctggcgg cgagctttgg tcaactcgga ttctgacagc gtggtcagtt cctggccatc
    75121 gaccagcacg ctaccctcgg ttgggcgctc cagcaggttt acacaacgta taagcgtact
    75181 cttacccgcg ccagaggcac cgataacgcc ataaatttgt ccagctggca catgcaggct
    75241 gacgttgttc aacgcctgga tggtgcgggt gccctggtgg aacactttgg tgatattcga
    75301 aagttttatc attgattatt tattatcgtc attaagttag tcgtggcatt tcgaatgcct
    75361 ggaacgggca acgccttcaa tgaaatggat gttaaggcat ccagacgtct aaatcaatca
    75421 ggtttatgcg aagagcactt tcttgctggt cgaaacatgc gatactagcg tcacatgcct
    75481 tattaaggag ctataaaagg tggcgaagag cgtacccgca atttttcttg accgtgatgg
    75541 caccattaat gtcgatcacg gctatgtcca tgagatcgac aactttgaat ttatcgacgg
    75601 tgttattgac gccatgcgcg agctaaaaaa aatgggcttt gcgctggtgg tagtaaccaa
    75661 ccagtctggc attgctcgcg gtaaatttac tgaagcacag tttgaaacgc tgaccgagtg
    75721 gatggactgg tcgctggcgg accgcgatgt cgatctggat ggtatctatt attgcccgca
    75781 tcatccgcag ggtagtgttg aagagtttcg ccaggtctgc gactgccgca aaccacatcc
    75841 ggggatgctt ttgtcagcac gcgattattt gcatattgat atggccgctt cttatatggt
    75901 gggcgataaa ttagaagata tgcaggcagc agctgcggcg aacgtgggaa caaaagtgct
    75961 ggtgcgtacg ggtaaacctg ttacgcctga agcagaaaac gcggcggatt gggtgttaaa
    76021 tagcctggca gacctgccgc aagcgataaa aaagcagcaa aaaccggcgt aatgattaaa
    76081 agatgagcgg ttgaaataaa aatgcatttt tccgcttgtc ttcctgagcc gactccctat
    76141 aatgcgcctc catcgacacg gcggatgtga atcacttcac acaaacagcc ggttcggtta
    76201 aagagaaaaa tcctgaaatt cagggttgac tctgaaagag gaaagcgtaa tatacgccac
    76261 ctcgcgacag tgagctgaaa gccgcgtcgc
//
LOCUS       DAZGQL010000008        76149 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.008, whole genome shotgun sequence.
ACCESSION   DAZGQL010000008 DAZGQL010000000
VERSION     DAZGQL010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 76149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 76149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..76149
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.008"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(47..1297)
                     /gene="icd"
                     /locus_tag="ACHKIR_000642"
     CDS             complement(47..1297)
                     /gene="icd"
                     /locus_tag="ACHKIR_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HFO9988432.1"
                     /translation="MESKVVVPAQGKKITLQNSKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            1469..2122
                     /gene="rluE"
                     /locus_tag="ACHKIR_000643"
     CDS             1469..2122
                     /gene="rluE"
                     /locus_tag="ACHKIR_000643"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HFO9988433.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTEDALEALRNGVTLNDGPTLPAGAEMVEEPEWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREATD"
     gene            2132..2593
                     /gene="nudJ"
                     /locus_tag="ACHKIR_000644"
     CDS             2132..2593
                     /gene="nudJ"
                     /locus_tag="ACHKIR_000644"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HFO9988434.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            2647..3753
                     /gene="mnmA"
                     /locus_tag="ACHKIR_000645"
                     /gene_synonym="trmU"
     CDS             2647..3753
                     /gene="mnmA"
                     /locus_tag="ACHKIR_000645"
                     /gene_synonym="trmU"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFO9988435.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILIVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYDGEVCLGGGIIEQRLPLPV"
     gene            3789..4430
                     /gene="hflD"
                     /locus_tag="ACHKIR_000646"
     CDS             3789..4430
                     /gene="hflD"
                     /locus_tag="ACHKIR_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HFO9988436.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            4434..5804
                     /gene="purB"
                     /locus_tag="ACHKIR_000647"
     CDS             4434..5804
                     /gene="purB"
                     /locus_tag="ACHKIR_000647"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFO9988437.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVASFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            5973..6644
                     /gene="phoP"
                     /locus_tag="ACHKIR_000648"
     CDS             5973..6644
                     /gene="phoP"
                     /locus_tag="ACHKIR_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HFO9988438.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            6644..8104
                     /gene="phoQ"
                     /locus_tag="ACHKIR_000649"
     CDS             6644..8104
                     /gene="phoQ"
                     /locus_tag="ACHKIR_000649"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HFO9988439.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            8180..9301
                     /gene="roxA"
                     /locus_tag="ACHKIR_000650"
                     /gene_synonym="ycfD"
     CDS             8180..9301
                     /gene="roxA"
                     /locus_tag="ACHKIR_000650"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HFO9988440.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETSWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(9447..10676)
                     /gene="pepT"
                     /locus_tag="ACHKIR_000651"
     CDS             complement(9447..10676)
                     /gene="pepT"
                     /locus_tag="ACHKIR_000651"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFO9988441.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRKS"
     gene            10926..12062
                     /gene="potA"
                     /locus_tag="ACHKIR_000652"
     CDS             10926..12062
                     /gene="potA"
                     /locus_tag="ACHKIR_000652"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HFO9988442.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPADEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFTVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            12046..12903
                     /gene="potB"
                     /locus_tag="ACHKIR_000653"
     CDS             12046..12903
                     /gene="potB"
                     /locus_tag="ACHKIR_000653"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HFO9988443.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            12900..13694
                     /gene="potC"
                     /locus_tag="ACHKIR_000654"
     CDS             12900..13694
                     /gene="potC"
                     /locus_tag="ACHKIR_000654"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HFO9988444.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            13691..14737
                     /gene="potD"
                     /locus_tag="ACHKIR_000655"
     CDS             13691..14737
                     /gene="potD"
                     /locus_tag="ACHKIR_000655"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HFO9988445.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLSNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            14795..15256
                     /locus_tag="ACHKIR_000656"
     CDS             14795..15256
                     /locus_tag="ACHKIR_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HFO9988446.1"
                     /translation="MSQDSRVFFRIFFGIGLVLILISVVIFYNQFTYSKDAIHTEGVI
                     VDTVWHSSHSHRTGKNGSWYPVVAFRPTPDYTLIFNSSIGSDFYEDSEGDKVNVYYSP
                     GHPEKAEINNPWVNFFKWGFIGIMGVIFISVGLLISMPYSKKTRRKRKSRP"
     gene            15253..16038
                     /locus_tag="ACHKIR_000657"
     CDS             15253..16038
                     /locus_tag="ACHKIR_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HFO9988447.1"
                     /translation="MKKIIILLSLLILLPLTVTSKPLIPIMKTLFTDVTGTVPDAEEI
                     ARKAELFRQQTGIAPFIVVVPGVNEASFRQNGKAMLAHAASSMSNVKGSVLLLFTTRE
                     PRLIMITNGQVESSMDDKHLGLLVENHTLAYLHADLWYQGINNALAVLQAQILKQPTP
                     PLTYYPHPGQQQENDLPGSTTTLGLFAWAVAFIVFAAFFNYTTRLYYALKFAVAMAVA
                     NMGYQALCLYIDNSFAITRISPLWAGLIGVCTFIAALLWTSKR"
     gene            complement(16164..16985)
                     /gene="cobB"
                     /locus_tag="ACHKIR_000658"
     CDS             complement(16164..16985)
                     /gene="cobB"
                     /locus_tag="ACHKIR_000658"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD+-dependent deacetylase"
                     /protein_id="HFO9988448.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQTFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(17001..17912)
                     /gene="nagK"
                     /locus_tag="ACHKIR_000659"
     CDS             complement(17001..17912)
                     /gene="nagK"
                     /locus_tag="ACHKIR_000659"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045127 - N-acetylglucosamine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HFO9988449.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADRRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(17941..19185)
                     /gene="lolE"
                     /locus_tag="ACHKIR_000660"
     CDS             complement(17941..19185)
                     /gene="lolE"
                     /locus_tag="ACHKIR_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HFO9988450.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVDQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(19185..19886)
                     /gene="lolD"
                     /locus_tag="ACHKIR_000661"
     CDS             complement(19185..19886)
                     /gene="lolD"
                     /locus_tag="ACHKIR_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HFO9988451.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(19879..21078)
                     /gene="lolC"
                     /locus_tag="ACHKIR_000662"
     CDS             complement(19879..21078)
                     /gene="lolC"
                     /locus_tag="ACHKIR_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HFO9988452.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            21340..22413
                     /locus_tag="ACHKIR_000663"
     CDS             21340..22413
                     /locus_tag="ACHKIR_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFO9988453.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHLTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLMSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATLMTYVKEVPLR
                     RHLLMTSLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGTN
                     TIAIYTTHRILVEIFSLTLLAPMNAARWSPQVELTLLMVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            22541..25987
                     /gene="mfd"
                     /locus_tag="ACHKIR_000664"
     CDS             22541..25987
                     /gene="mfd"
                     /locus_tag="ACHKIR_000664"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFO9988454.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEEINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLENSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLESFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDTVRNLALICESDLL
                     GERVARRRQDSRRAINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            26131..27093
                     /gene="ldtC"
                     /locus_tag="ACHKIR_000665"
     CDS             26131..27093
                     /gene="ldtC"
                     /locus_tag="ACHKIR_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HFO9988455.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPAGSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(27176..27433)
                     /gene="bhsA"
                     /locus_tag="ACHKIR_000666"
                     /gene_synonym="ycfR"
     CDS             complement(27176..27433)
                     /gene="bhsA"
                     /locus_tag="ACHKIR_000666"
                     /gene_synonym="ycfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HFO9988456.1"
                     /translation="MKNVKTLIAAAILSSMSFASFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            27674..28306
                     /gene="comR"
                     /locus_tag="ACHKIR_000667"
     CDS             27674..28306
                     /gene="comR"
                     /locus_tag="ACHKIR_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HFO9988457.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIARTTLRLWPELVK"
     gene            complement(28368..28907)
                     /locus_tag="ACHKIR_000668"
     CDS             complement(28368..28907)
                     /locus_tag="ACHKIR_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFO9988458.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(29134..30438)
                     /gene="ndh"
                     /locus_tag="ACHKIR_000669"
     CDS             complement(29134..30438)
                     /gene="ndh"
                     /locus_tag="ACHKIR_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HFO9988459.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHSELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(30838..31380)
                     /gene="ycfP"
                     /locus_tag="ACHKIR_000670"
     CDS             complement(30838..31380)
                     /gene="ycfP"
                     /locus_tag="ACHKIR_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase YcfP"
                     /protein_id="HFO9988460.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(31403..32428)
                     /gene="nagZ"
                     /locus_tag="ACHKIR_000671"
     CDS             complement(31403..32428)
                     /gene="nagZ"
                     /locus_tag="ACHKIR_000671"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HFO9988461.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAREAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHSDPEKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISARLNQLHERW
                     QEEKAGH"
     gene            complement(32439..33263)
                     /gene="thiK"
                     /locus_tag="ACHKIR_000672"
     CDS             complement(32439..33263)
                     /gene="thiK"
                     /locus_tag="ACHKIR_000672"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019165 - thiamine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HFO9988462.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPRFAFLRQYRALSQLPACIAPKPHLYLRDWMVVDYLPGEVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRRLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTDQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(33244..33885)
                     /gene="lpoB"
                     /locus_tag="ACHKIR_000673"
     CDS             complement(33244..33885)
                     /gene="lpoB"
                     /locus_tag="ACHKIR_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HFO9988463.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(33899..34276)
                     /locus_tag="ACHKIR_000674"
     CDS             complement(33899..34276)
                     /locus_tag="ACHKIR_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HFO9988464.1"
                     /translation="MRKGCFGLVSLALLVLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTLPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(34279..34638)
                     /gene="hinT"
                     /locus_tag="ACHKIR_000675"
     CDS             complement(34279..34638)
                     /gene="hinT"
                     /locus_tag="ACHKIR_000675"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HFO9988465.1"
                     /translation="MAEETIFSKIIRREITSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            34972..37161
                     /gene="fhuE"
                     /locus_tag="ACHKIR_000676"
     CDS             34972..37161
                     /gene="fhuE"
                     /locus_tag="ACHKIR_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HFO9988466.1"
                     /translation="MLSTQFNRDNQYQTTSKPSLLAACIALALLPSAAFAAPATEETV
                     IVEGSATTPVDDENDYSVTSTSAGTKMQMTQRDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQRIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLVGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGSYEL
                     FGRQHNLMFGGSYSKQNNRYFSSWAYIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNVEGSIVYGAPRNFSITGTYQF"
     gene            complement(37221..38654)
                     /gene="ptsG"
                     /locus_tag="ACHKIR_000677"
     CDS             complement(37221..38654)
                     /gene="ptsG"
                     /locus_tag="ACHKIR_000677"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005355 - glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:1904659 - glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HFO9988467.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(38949..39746)
                     /locus_tag="ACHKIR_000678"
     CDS             complement(38949..39746)
                     /locus_tag="ACHKIR_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO9988468.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPQDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(39757..40761)
                     /gene="holB"
                     /locus_tag="ACHKIR_000679"
     CDS             complement(39757..40761)
                     /gene="holB"
                     /locus_tag="ACHKIR_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFO9988469.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFHGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVVE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(40758..41399)
                     /gene="tmk"
                     /locus_tag="ACHKIR_000680"
     CDS             complement(40758..41399)
                     /gene="tmk"
                     /locus_tag="ACHKIR_000680"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFO9988470.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATLPLEAVMDAIRTTVTNWVKELDA"
     gene            complement(41389..42411)
                     /gene="yceG"
                     /locus_tag="ACHKIR_000681"
     CDS             complement(41389..42411)
                     /gene="yceG"
                     /locus_tag="ACHKIR_000681"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HFO9988471.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKLRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(42414..43223)
                     /gene="pabC"
                     /locus_tag="ACHKIR_000682"
     CDS             complement(42414..43223)
                     /gene="pabC"
                     /locus_tag="ACHKIR_000682"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HFO9988472.1"
                     /translation="MFLINGHKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMISCDFWPQLELEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTVYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(43343..44584)
                     /gene="fabF"
                     /locus_tag="ACHKIR_000683"
     CDS             complement(43343..44584)
                     /gene="fabF"
                     /locus_tag="ACHKIR_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFO9988473.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(44672..44908)
                     /gene="acpP"
                     /locus_tag="ACHKIR_000684"
     CDS             complement(44672..44908)
                     /gene="acpP"
                     /locus_tag="ACHKIR_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFO9988474.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(45119..45853)
                     /gene="fabG"
                     /locus_tag="ACHKIR_000685"
     CDS             complement(45119..45853)
                     /gene="fabG"
                     /locus_tag="ACHKIR_000685"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HFO9988475.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ITDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(45866..46795)
                     /gene="fabD"
                     /locus_tag="ACHKIR_000686"
     CDS             complement(45866..46795)
                     /gene="fabD"
                     /locus_tag="ACHKIR_000686"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFO9988476.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVPVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(46811..47764)
                     /gene="fabH"
                     /locus_tag="ACHKIR_000687"
     CDS             complement(46811..47764)
                     /gene="fabH"
                     /locus_tag="ACHKIR_000687"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFO9988477.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(47832..48902)
                     /gene="plsX"
                     /locus_tag="ACHKIR_000688"
     CDS             complement(47832..48902)
                     /gene="plsX"
                     /locus_tag="ACHKIR_000688"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFO9988478.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRVALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(48983..49156)
                     /gene="rpmF"
                     /locus_tag="ACHKIR_000689"
     CDS             complement(48983..49156)
                     /gene="rpmF"
                     /locus_tag="ACHKIR_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFO9988479.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(49208..49729)
                     /gene="yceD"
                     /locus_tag="ACHKIR_000690"
     CDS             complement(49208..49729)
                     /gene="yceD"
                     /locus_tag="ACHKIR_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HFO9988480.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEAQK
                     PNPFAVLASLKRK"
     gene            49928..50512
                     /gene="yceF"
                     /locus_tag="ACHKIR_000691"
     CDS             49928..50512
                     /gene="yceF"
                     /locus_tag="ACHKIR_000691"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HFO9988481.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(50625..51584)
                     /gene="rluC"
                     /locus_tag="ACHKIR_000692"
     CDS             complement(50625..51584)
                     /gene="rluC"
                     /locus_tag="ACHKIR_000692"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HFO9988482.1"
                     /translation="MKTETPSVKIVAINADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            52157..55342
                     /gene="rne"
                     /locus_tag="ACHKIR_000693"
     CDS             52157..55342
                     /gene="rne"
                     /locus_tag="ACHKIR_000693"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008995 - ribonuclease E activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HFO9988483.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAAPVVAPAPKAATATPASPA
                     QPGLLSRFFGALKALFSGGEETKPTEQPAPKAEAKPERQQDRRKPRQNNRRDRNERRD
                     TRSERAEGNDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETVAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEVAEVVEAPVQVAEPQPEVAETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            55476..55769
                     /locus_tag="ACHKIR_000694"
     CDS             55476..55769
                     /locus_tag="ACHKIR_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFO9988484.1"
                     /translation="MPAANNIPARRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEA
                     ARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPNAALRKTMQTPAPWEQKK"
     gene            55766..56260
                     /locus_tag="ACHKIR_000695"
     CDS             55766..56260
                     /locus_tag="ACHKIR_000695"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO9988485.1"
                     /translation="MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTF
                     VSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEPIPVVVLGRLAIDKSLQGQGIDR
                     AMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
                     ECLK"
     gene            complement(56355..57308)
                     /gene="flgL"
                     /locus_tag="ACHKIR_000696"
     CDS             complement(56355..57308)
                     /gene="flgL"
                     /locus_tag="ACHKIR_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HFO9988486.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTESAPFSEADGKYEGGAESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKETAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(57320..58963)
                     /gene="flgK"
                     /locus_tag="ACHKIR_000697"
     CDS             complement(57320..58963)
                     /gene="flgK"
                     /locus_tag="ACHKIR_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HFO9988487.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITMANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQDLDQTRNTLGQLALAFAEAFNTQHKAGFDANGDAGEDFFAIGKPA
                     VLQNTKNKGDVAIGATVTDASAVLATDYKISFDNNQWQVTRLASNTTFTVTPDASGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASAEDTGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSTTQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(59029..59970)
                     /gene="flgJ"
                     /locus_tag="ACHKIR_000698"
     CDS             complement(59029..59970)
                     /gene="flgJ"
                     /locus_tag="ACHKIR_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HFO9988488.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKAGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQTLSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTSMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(59970..61067)
                     /gene="flgI"
                     /locus_tag="ACHKIR_000699"
     CDS             complement(59970..61067)
                     /gene="flgI"
                     /locus_tag="ACHKIR_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HFO9988489.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(61079..61777)
                     /gene="flgH"
                     /locus_tag="ACHKIR_000700"
     CDS             complement(61079..61777)
                     /gene="flgH"
                     /locus_tag="ACHKIR_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HFO9988490.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(61830..62612)
                     /gene="flgG"
                     /locus_tag="ACHKIR_000701"
     CDS             complement(61830..62612)
                     /gene="flgG"
                     /locus_tag="ACHKIR_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HFO9988491.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(62783..63538)
                     /gene="flgF"
                     /locus_tag="ACHKIR_000702"
     CDS             complement(62783..63538)
                     /gene="flgF"
                     /locus_tag="ACHKIR_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HFO9988492.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLAVQTADGSEGYTRN
                     GSIQVDPTGQLTIQGHPVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAETQTTRGPVLQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(63558..64763)
                     /gene="flgE"
                     /locus_tag="ACHKIR_000703"
     CDS             complement(63558..64763)
                     /gene="flgE"
                     /locus_tag="ACHKIR_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HFO9988493.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPSVNAFDASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     TGTAEPAMTLVFNANGVLTSDPTANISTGAINGAEPATFSLSFLNSMQQNTGANNIVA
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSA
                     TQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            complement(64788..65483)
                     /gene="flgD"
                     /locus_tag="ACHKIR_000704"
     CDS             complement(64788..65483)
                     /gene="flgD"
                     /locus_tag="ACHKIR_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HFO9988494.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGTTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNNGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(65495..65899)
                     /gene="flgC"
                     /locus_tag="ACHKIR_000705"
     CDS             complement(65495..65899)
                     /gene="flgC"
                     /locus_tag="ACHKIR_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HFO9988495.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(65903..66319)
                     /gene="flgB"
                     /locus_tag="ACHKIR_000706"
     CDS             complement(65903..66319)
                     /gene="flgB"
                     /locus_tag="ACHKIR_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HFO9988496.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            66475..67134
                     /gene="flgA"
                     /locus_tag="ACHKIR_000707"
     CDS             66475..67134
                     /gene="flgA"
                     /locus_tag="ACHKIR_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HFO9988497.1"
                     /translation="MLAIKRSVAIIAILFSPLSAASNLTSQLHTFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            67210..67503
                     /gene="flgM"
                     /locus_tag="ACHKIR_000708"
     CDS             67210..67503
                     /gene="flgM"
                     /locus_tag="ACHKIR_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HFO9988498.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNTRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            67508..67924
                     /gene="flgN"
                     /locus_tag="ACHKIR_000709"
     CDS             67508..67924
                     /gene="flgN"
                     /locus_tag="ACHKIR_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HFO9988499.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRRKEPNAANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGSKKISI"
     gene            complement(67964..69499)
                     /gene="murJ"
                     /locus_tag="ACHKIR_000710"
     CDS             complement(67964..69499)
                     /gene="murJ"
                     /locus_tag="ACHKIR_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFO9988500.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTFALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(69609..70532)
                     /locus_tag="ACHKIR_000711"
     CDS             complement(69609..70532)
                     /locus_tag="ACHKIR_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFO9988501.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWMLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVHKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGEQAVRAQRIVDKIWRDAMSE"
     gene            complement(70534..71181)
                     /locus_tag="ACHKIR_000712"
     CDS             complement(70534..71181)
                     /locus_tag="ACHKIR_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HFO9988502.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVMDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(71192..71776)
                     /gene="rimJ"
                     /locus_tag="ACHKIR_000713"
     CDS             complement(71192..71776)
                     /gene="rimJ"
                     /locus_tag="ACHKIR_000713"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HFO9988503.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            72012..73220
                     /gene="mdtH"
                     /locus_tag="ACHKIR_000714"
     CDS             72012..73220
                     /gene="mdtH"
                     /locus_tag="ACHKIR_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HFO9988504.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            73284..73931
                     /gene="grxB"
                     /locus_tag="ACHKIR_000715"
     CDS             73284..73931
                     /gene="grxB"
                     /locus_tag="ACHKIR_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HFO9988505.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPIELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            74065..74625
                     /locus_tag="ACHKIR_000716"
     CDS             74065..74625
                     /locus_tag="ACHKIR_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO9988506.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            74731..75777
                     /gene="pyrC"
                     /locus_tag="ACHKIR_000717"
     CDS             74731..75777
                     /gene="pyrC"
                     /locus_tag="ACHKIR_000717"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFO9988507.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSVDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVRKEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            75851..76096
                     /gene="dinI"
                     /locus_tag="ACHKIR_000718"
     CDS             75851..76096
                     /gene="dinI"
                     /locus_tag="ACHKIR_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HFO9988508.1"
                     /translation="MRIEVTIAKTSLLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
BASE COUNT        18414 a        20256 c        19111 g        18368 t
ORIGIN      
        1 aacgggcgtg ttatacgccc gtttcaatat ttaacacatg tagagattac atgttcttga
       61 tgatcgcatc accaaactcg ctgcatttca gcagtttagc gccttccatc agacgttcga
      121 agtcataggt tacagtcttg gcgttgattg cgccttccat acctttaaca atcaggtctg
      181 cggcttcagt ccagcccata tggcgtaaca tcatctcagc ggagagaata atagagccag
      241 ggttaacttt gtcctgaccg gcatatttcg gcgcagtacc gtgggtggct tcaaacaggg
      301 cgcattcgtc accgatgttt gcgcctgggg cgataccgat accgccgacc tgtgcagcca
      361 gagcgtcaga aatgtagtca ccgttcaggt tcatacaggc gataacatca tattcagccg
      421 gacgcagcag gatctgttgc aggaatgcat cagcaatcac atctttaatg acgatctctt
      481 tgccggtatt cgggttttta actttcagcc acgggccgcc gtcgatcagt tcaccgccaa
      541 actcttcacg cgccagctgg tagccccaat ctttaaacgc gccttcggtg aacttcatga
      601 tgttgccttt gtgcaccagg gtcacagagt cacgatcgtt cgcaattgcg tattcgatcg
      661 ctgcacgcac cagacgtttg gtgccttctt cagaacacgg cttaataccg ataccgcaat
      721 gttccgggaa acgaattttc ttcacgccca tctcttcacg caggaattta atcactttct
      781 cggcgtcggc agagtctgct ttccattcga tacccgcata aatgtcttcc gagttttcac
      841 ggaagataac catatcggtc agttcagggt gtttaaccgg gcttggggtg ccctggtagt
      901 aacgtaccgg acgcaggcag atgtagagat ccagttcctg gcgcagggca acgttcagag
      961 agcgaatacc gccaccaacc ggagtggtca gcgggccttt aatggcaacg cgatattcac
     1021 gaatcagatc aagggtttca gcaggcagcc agacatcctg accataaacc tgtgtggatt
     1081 tttcaccggt gtaaatttcc atccaggaga ttttacgctc gcctttatag gctttctcga
     1141 ctgcagcgtc gaccactttc agcatggctg gggttacatc tacaccgatt ccatcacctt
     1201 caatgtaagg gataatcgga ttttcaggaa cgttgagttt gctgttttgc agggtgatct
     1261 tcttgccttg tgccggaaca actactttac tttccattca cctctccttc gagcgctact
     1321 ggtttgctcg tctgccacca cgttgcatat gcgtttgcgt cctgcgatac ggatgcttta
     1381 gagcaatttt ttgttaatga tttgtaattg gcttgtcaat actaccctat tgtttgccgt
     1441 catgaaagat gcgcgttatt aggctataat gcggcaattc ataatctctg aaaataccat
     1501 gcaaaaaact tcttttagaa atcaccaggt taagcgattc agctcgcaac gttctaccag
     1561 gcgtaaacct gaaaaccagc ccacgcgtgt gatcctgttc aataaaccct acgatgttct
     1621 tccgcagttc accgatgaag ccggacgcaa aacattaaaa gaattcatcc cggttcaagg
     1681 cgtttatgca gcaggtcgcc ttgaccgcga tagcgaaggg ttactggtgc tgaccaataa
     1741 cggcgcgttg caggcgcgtt taacccagcc gggtaaacgt accgggaaaa tctattatgt
     1801 acaggtagaa ggtattccca cggaagacgc ccttgaagcg ttgcgcaatg gcgtaacctt
     1861 aaatgatggc cctaccctgc ccgcaggtgc agagatggtt gaggagccgg aatggttatg
     1921 gccccgtaat ccgccaattc gtgaacgcaa aagtattccc accagctggc tgaagatcac
     1981 cttatatgaa ggacgaaatc gccaggtgcg ccgcatgacc gcccatgttg gtttccccac
     2041 gctgcgactg attcgctatg cgatgggtga ttactctttg gataatcttg ccaatggcga
     2101 atggcgagaa gcgacagatt aaggacgcaa aatgtttaaa ccgcacgtta ccgttgcctg
     2161 cgtggtgcac gcagaaggca aatttttagt cgttgaagag acgattaatg gtaaagcgtt
     2221 atggaaccaa cctgccgggc atctggaagc ggatgagacg ctagtggaag ctgctgcgcg
     2281 tgagctgtgg gaagaaaccg gcatcagcgc gcagccgcaa cactttattc gtatgcatca
     2341 gtggattgcg ccagataaaa cgccgttttt gcgtttcctg tttgctattg agcttgagca
     2401 aatatgcccg acccagcctc atgacagcga tatcgactgc tgccgttggg tcagcgccga
     2461 agaaatttta caggcgtcaa atctgcgttc gccgctggtg gcggaaagta ttcgttgtta
     2521 tcaaagcggg caacgctatc cgctggagat gattggcgat tttaactggc cttttacaaa
     2581 gggtgtcatc taaaaggctg cgtgatagaa tacgccgcct tgaagttcaa tgtcgtgagt
     2641 gatccaatgt ctgaaaccgc aaaaaaagta atcgtcggca tgtccggcgg tgtcgattcc
     2701 tccgtttctg cctggctgtt gcaacaacag ggatatcagg tcgaaggcct gtttatgaag
     2761 aactgggaag aagacgacgg tgaggaatat tgcacagcgg cagcggatct ggctgatgcc
     2821 caggctgtct gcgacaagct cggcattgaa ctgcacaccg ttaactttgc tgccgagtac
     2881 tgggacaacg tcttcgaact gttccttgcc gaatataaag ccggtcgcac gccgaatccg
     2941 gatattctgt gcaacaaaga gatcaaattt aaagccttcc tcgaatttgc cgccgaagat
     3001 ttaggtgccg attatatcgc taccggtcat tacgtacgtc gggcagatgt cgatggcaag
     3061 agccgcctgc tgcgtggtct ggacagcaat aaagaccaga gctacttcct ttatacgctc
     3121 agccatgagc agattgcgca aagcctgttc ccggtcggcg aactggaaaa accgcaggtg
     3181 cgtaagattg ctgaagatct tggtctggtc accgcgaaga aaaaagactc taccggcatt
     3241 tgcttcattg gcgaacgtaa attccgcgag ttcctgggcc gttatctccc ggcgcaaccg
     3301 ggcaaaatca ttaccgtcga tggcgatgaa attggtgaac accaggggct gatgtatcac
     3361 accctcggtc agcgtaaagg tctgggtatt ggtggcacca aagaaggcac cgaagaaccg
     3421 tggtatgtgg tcgataaaga cgtcgaaaac aacattctga ttgttgcgca gggccatgaa
     3481 cacccgcgtc tgatgtctgt cggtctgatt gcccagcagt tgcactgggt cgatcgcgaa
     3541 ccattcaccg gcactatgcg ttgcacggta aaaacccgct atcgccagac cgacattccg
     3601 tgcaccgtca aggcgctgga cgatgatcgc attgaagtga ttttcgatga gccggttgcc
     3661 gccgtgacgc cgggccagtc tgccgtattc tatgacggtg aagtgtgcct cggtggcggt
     3721 attattgagc agcgtctgcc gctgccggtc tgattatttt ctttacttaa aacaaggaag
     3781 cagtgaacgt ggcaaagaat tactatgaca tcaccctcgc tctggccggt atttgtcagt
     3841 cggcacgcct ggtgcaacaa ctcgctcacc aggggcattg tgatgccgat gcgctacacg
     3901 tctcactcaa cagtattatt gatatgaacc ccagctcgac gctggcggtt tttggcggta
     3961 gcgaagccaa cctgcgcgtc gggctggaaa ccttgctcgg cgtgctcaat gccagcagtc
     4021 gccagggctt aaacgctgag ttaacccgct acacactcag cctgatggtg cttgagcgca
     4081 aactctcctc agcgaaaggt gcgctcgaca ctctgggcaa ccggatcaac ggcctgcaac
     4141 gccagctcga acacttcgat ttacagtccg aaacgctgat gagcgcgatg gctgctatct
     4201 atgttgatgt gattagcccg cttggcccgc gcattcaggt caccggttcc cctgctgtac
     4261 tgcaaagccc acaagtgcag gcgaaagttc gcgctaccct gctggcaggc attcgcgccg
     4321 ccgtgctctg gcaccaggtc ggcggcggac gtctgcaact gatgttttct cgtaatcgcc
     4381 tgaccactca ggcaaaacaa attcttgctc atttaacccc ggagttgtga tctatggaat
     4441 tatcctcact gaccgccgtt tcccctgtcg atggacgcta cggcgataaa gtcagcgcgc
     4501 tgcgcgggat tttcagcgaa tatggtttgc tgaaattccg tgtacaagtt gaagtacgtt
     4561 ggctgcaaaa actggccgcg cacgcagcga tcaaggaagt tcctgctttt gctgccgacg
     4621 caatcggtta ccttgatgca attgtcgcca gtttcagcga agaagatgca gcacgcatca
     4681 aaaccatcga gcgtaccact aaccacgacg ttaaagcggt tgagtatttc ctgaaagaaa
     4741 aagtggcgga gatcccggaa ctgcatgcgg tttctgaatt catccacttt gcctgtactt
     4801 cggaagatat caataacctc tcccacgcgt tgatgctgaa aaccgcgcgt gatgaagtga
     4861 tcctgccgta ctggcgtcaa ctgattgatg gcattaaaga tctcgccgtt cagtatcgcg
     4921 atatcccgct gctgtcccgt acccacggtc agccagccac gccgtcaacc atcggtaaag
     4981 agatggctaa cgtcgcctac cgtatggagc gccagtaccg ccagcttaat caggtggaga
     5041 tcctcggtaa aatcaacggc gcagtcggta actataacgc ccacatcgcc gcttacccgg
     5101 aagttgactg gcatcagttc agcgaagagt tcgtcacctc gctgggtatt cagtggaacc
     5161 cgtacactac ccagatcgaa ccgcacgact acattgccga gctgtttgat tgcgttgcgc
     5221 gcttcaacac cattctgatc gactttgacc gtgacgtctg gggttatatc gcccttaacc
     5281 acttcaaaca gaaaaccatt gctggtgaga ttggttcttc caccatgccg cataaagtta
     5341 acccgatcga cttcgaaaac tccgaaggga atctgggcct ttccaacgcg gtattgcagc
     5401 atctggcaag caaactgccg gtttcccgct ggcagcgtga cctgaccgac tccaccgtgc
     5461 tgcgtaacct cggcgtgggt atcggttatg cgctgattgc gtatcaatcc accctgaaag
     5521 gggtgagcaa actggaagtg aaccgtgacc atctgctgga tgaactggat cacaactggg
     5581 aagtgctggc tgagccaatc cagacagtta tgcgtcgcta tggcatcgaa aaaccgtacg
     5641 agaagctgaa agagctgact cgcggtaagc gcgttgacgc cgaaggcatg aagcagttta
     5701 tcgacggtct ggcgttgcca gaagaagaga aagcccgcct gaaagcgatg acgccggcta
     5761 actatattgg tcgcgccatc accatggttg atgagctgaa ataagcctca aatcagtgcc
     5821 ggatggcgat gctgtccggc ctgcttatta agattatcca ctttttgttt tttcacttta
     5881 cctccccttc ccgctggttt atttaatgtt tacccccata accacataat cgcgttacac
     5941 tattttaata attaagacag ggagaaataa aaatgcgcgt actggttgtt gaagacaatg
     6001 cgttgttacg tcaccatctt aaagttcaga ttcaggatgc tggtcatcag gtcgatgacg
     6061 cagaagatgc caaagaagcc gattattatc tcaatgaaca tttaccggat attgcgattg
     6121 tcgatctcgg tttgcctgac gaggacggtc tgtcactgat tcgccgctgg cgtagcaatg
     6181 atgtttcact gccgattctg gtattaaccg cccgtgaaag ctggcaggac aaagtcgaag
     6241 tattaagtgc tggcgctgat gattacgtga ctaaaccgtt tcatattgaa gaggtgatgg
     6301 cgcgaatgca ggcattaatg cggcgtaata gcggtctggc ttcacaggtc atttcgctac
     6361 caccgtttca ggttgatctc tctcgccgtg aattatctat taatgacgaa gtgatcaaac
     6421 tgaccgcgtt cgaatacacc attatggaaa cgttgatacg caataatggc aaagtggtga
     6481 gcaaagattc gttaatgctc caactctatc cggatgcgga gctgcgggaa agccatacca
     6541 ttgatgtact gatgggacgt ctgcgcaaaa aaattcaggc acaatatccg caagaagtga
     6601 ttaccaccgt tcgcggccag ggctatctgt tcgaattgcg ctgatgaaaa aattactgcg
     6661 tctttttttc ccgctctcgc tgcgggtacg ttttctgttg gcaacggcag cggtagtact
     6721 ggtgctttcg cttgcctacg gaatggtcgc gctgatcggt tatagcgtca gtttcgataa
     6781 aaccacgttt cggctgttac gtggcgagag caatctgttc tatacccttg cgaagtggga
     6841 aaacaataag ttgcatgtcg agttacccga aaatatcgac aagcaaagcc ccacgatgac
     6901 actaatttat gatgagaacg ggcagctttt atgggcgcaa cgtgacgtgc cctggctgat
     6961 gaagatgatc cagcctgact ggctgaaatc gaatggtttt catgaaattg aagcggatgt
     7021 taacgatacc agcctcttgc tgagtggaga tcattcgata cagcaacagt tgcaggaagt
     7081 gcgggaagat gatgacgacg cggagatgac ccactcggtg gcggtaaacg tctacccggc
     7141 aacatcgcgg atgccaaagt tgaccattgt ggtggtggat accattccgg tggagctaaa
     7201 aagttcctat atggtctgga gctggtttat ctatgtgctc tcagccaatc tgctgttagt
     7261 gatcccgctg ctgtgggtcg ccgcctggtg gagtttacgc cccatcgaag ccctggcaaa
     7321 agaagtccgc gaactggaag aacataaccg cgaattgctc aatccagcca caacgcgaga
     7381 actgaccagt ctggtacgaa acctgaaccg attgttaaaa agtgaacgcg aacgttacga
     7441 caaatatcgt acgacgctca ccgacctgac ccatagtctg aaaacgccac tggcggtgct
     7501 gcaaagtacg ctgcgttctc tgcgtagtga aaagatgagc gtcagtgatg ctgaaccggt
     7561 aatgctggag caaatcagcc gcatttcaca gcaaattggc tactacctgc atcgtgccag
     7621 tatgcgcggc gggacattgc tcagccgcga gctgcatccg gtcgctccac tgctggacaa
     7681 tctcacctcg gcgctgaaca aagtgtatca acgcaaaggg gtcaatatct ctctcgatat
     7741 ttcgccagag atcagctttg tcggtgagca gaacgatttt gtcgaggtga tgggcaatgt
     7801 gctggataat gcctgtaaat attgcctcga gtttgtcgaa atttctgcaa ggcaaaccga
     7861 cgagcatctc tatattgtgg tcgaggatga tggccccggt attccattaa gcaagcgaga
     7921 ggtcattttc gaccgtggtc aacgggttga tactttacgc cctgggcaag gtgtggggct
     7981 ggcggtagcc cgcgaaatca ccgagcaata tgagggtaaa atcgtcgccg gagagagcat
     8041 gctgggcggt gcgcggatgg aggtgatttt tggtcgccag cattctgcgc cgaaagatga
     8101 ataaatatgt ccatacttca cgcattacgt taagcatccg ttataatcgg ttgcagatac
     8161 cagcctgtgg atgcttaaca tggaatacca actcactctt aactggcccg attttcttga
     8221 acgtcactgg cagaaacgcc cggtggtgtt aaaacgcggc tttaataatt ttattgaccc
     8281 gatctctcca gacgagttgg cgggtctggc gatggaaagc gaagtcgaca gtcgactggt
     8341 cagtcatcag gatggcaaat ggcaggtcag ccacggtcca ttcgaaagct acgatcatct
     8401 cggtgaaact agctggtcat tgttagtgca agcagtgaac cactggcatg agccgaccgc
     8461 cgcgctgatg cgaccgttcc gtgaactgcc ggactggcgt attgatgacc tgatgatctc
     8521 tttttctgta cctggcggcg gcgtcggccc gcatctcgat cagtacgacg tgtttatcat
     8581 tcagggtacc ggacgtcgtc gctggcgagt gggcgaaaag ctgcaaatga aacagcactg
     8641 cccacatccg gatctgttac aggtcgatcc gttcgaagcc atcatcgatg aagagctgga
     8701 gcctggtgat attctttata ttccgccagg attcccgcat gaaggctacg cgctggaaaa
     8761 tgcgatgaac tattccgtgg gttttcgcgc gccaaatacg cgggaactga ttagtggatt
     8821 tgccgattat gtgctgcaac gtgaactggg cggcaactac tacagcgatc cggatgttcc
     8881 acctcgcgct catcctgcgg acgttctgcc gcaagagatg gataaactgc gtgagatgat
     8941 gctcgaattg atcaaccagc cggaacattt taagcaatgg tttggcgagt ttatatccca
     9001 gtcacgtcat gaactggata tcgcgccgcc agagccgcct tatcagccgg atgaaatcta
     9061 cgatgcgctg aaacaaggtg atgtgctggt gcgcctgggg ggtctgcgcg tattgcgcat
     9121 tggcgacgac gtgtatgcca atggtgagaa gatcgattcc ccgcaccgtc cggcactgga
     9181 tgcgctcgcc agcaacattg cgctgaccgc ggagaatttt ggcgatgcgc tggaagatcc
     9241 gtcattcctc gcgatgctcg cggcgctggt caatagcgga tactggttct tcgaagggta
     9301 agtttggatt taagccggat gcggcgctga ttgcgcctta tcgtgcctac atgcactgaa
     9361 cgcctgtagg cacgataagt atgtggacca gtgtcaatat aaaggtttca tcccggtaat
     9421 tggcagtcca catacgcatc gggcaatcac gacttccgtt gcgccgtcaa ctcggcaata
     9481 cggacgatca cctgcaccgc tttttccata ccttccagag tcacaaactc atgcttacca
     9541 tgatagttgt aaccgccagt gaacaggttc gggcacggta atcccataaa cgacaactgc
     9601 gcgccgtcgg taccgccgcg gatcggtttc agttccggtt caatatcgca gtcacgcatc
     9661 gcctgctggg cgatatcgag aatatgcgga tgctcaacca ctttctcgcg catattgtag
     9721 taactgtctt caatcaccag ttcaatgtag caatcaggat gtaacccttt gcccactttt
     9781 ttggcgatct ccatcatttt acgtttacgc gcttcaaact gtttacggtc gaaatcacgg
     9841 atgatgtagt gcatatcggc ccgttcaacg gtgcctttca tgctcgccag gtgatagaaa
     9901 ccttcatagc cttctgtcat ttccgggctt tcatccgccg gaacttccgc atgaatacgt
     9961 gccgccagcg acagcgcatt taccattact cctttcgccg tgcccgggtg aacattgtta
    10021 ccgacaattt tgatattgac cgacgcggcg ttgaagtttt caaactccag ttcgcctacg
    10081 ccaccaccat caacggtgta agcccagcgg gcatcgaagg cgtcaacatc aaaatgcttc
    10141 gcccctttgc ccacttcttc atccggggta aaggcgacgc gaatatcacc atgcggaatg
    10201 tttttttgtt gcaataccgc cagcgcggtc atgatttctg caatacctgc tttgtcatcg
    10261 gcacctaaca aggttttacc atcggtggta atcagcgtct gacccagtag ctgatgcagc
    10321 accgggaaca taaccggtga taaaacttca tcgccgatac ccagcgcaat atcgccaccg
    10381 cgatagtttt caacaatttg cggattcaca tttttgccgc tgcaatccgg tgaggtatcc
    10441 acatgagaaa taaagccaat cgccgggata tcgccaggga cgttagccgg taacgtcgcc
    10501 atcaaagtgc ccttctcact taaggtcaca ttgataagcc ccatctcttc gagctgctct
    10561 ttcagcagat gcaataactt ccattggcct tccgtgctgg gaacctgtct cacccctgcc
    10621 tttgattggg tatccagaga cacgtagttc aaaaatcgct caagtagttt atccatgtag
    10681 tcaccctcac tttttgtgac aacattatta agaagcaaga aaagacaaat attgcgtcag
    10741 gtcactttta gccctgcaag cgggaatatt tatcagcatt accttaatga atataaagct
    10801 aagccagtaa ttcacaacaa agattggcga ttcaagcggt ttgccgtaga attaccgccc
    10861 tcattaagag tcaccaaggt ggttaaccac aaaccccgca tcggtaagcc atccgttgcg
    10921 tttacatggg acagagtaaa aaattgaata aacaaccgag ttcgctttca ccgctggtgc
    10981 aattggcggg aattcgcaaa tgctttgatg gtaaagaggt cattccccag ctggatctga
    11041 ctatcaacaa tggcgagttc ctcacgctgc ttggcccttc tggctgcggt aaaacaaccg
    11101 ttcttcgcct gattgcaggt ctggaaactg ttgattccgg acgcatcatg ttggataacg
    11161 aggacatcac ccacgttccg gcggaaaacc gctacgtgaa cactgttttc caaagctacg
    11221 cacttttccc ccacatgacc gtgttcgaaa atgtggcctt tgggttgcgc atgcaaaaaa
    11281 cccccgctga tgaaattacg ccccgcgtga tggaagccct gcggatggtg cagctggaaa
    11341 ccttcgctca acgcaaaccg catcagctct ctggtggtca acagcaacgc gtcgccattg
    11401 cccgcgcggt ggttaacaag cctcgtctgt tgttgctgga tgagtcgctc tcagcgctgg
    11461 attacaaact gcgcaagcaa atgcagaacg agctgaaagc gttacagcgt aagcttggta
    11521 ttacattcgt gttcgtaact cacgaccagg aagaagcact taccatgtcg gacaggatcg
    11581 tggtgatgcg cgatggtcgc attgagcaag acggcacgcc gcgtgaaatc tacgaagagc
    11641 cgaaaaacct gtttgttgcc gggtttattg gcgaaatcaa tatgtttaac gccactgtca
    11701 tcgaacgtct cgacgagcag cgcgtacgcg ccaacgttga aggccgcgaa tgtaatatct
    11761 acgttaactt caccgttgaa ccggggcaaa aactgcatgt tctgctgcgc ccggaagact
    11821 tacgtgttga agagattaac gacgacaacc acgccgaagg gctgattggt tacgttcgcg
    11881 agcgtaacta caaaggcatg acgctggagt cggttgttga gctggaaaat ggcaagatgg
    11941 tgatggtcag cgaattcttc aatgaagacg atcctgactt tgaccactct ctcgaccaaa
    12001 aaatggccat taattgggta gaaagctggg aggtcgtact ggctgatgaa gaacacaagt
    12061 aagttccaga atgtagtgat tgtcactatt gtcggttggc ttgtgttgtt tgtctttctg
    12121 cccaacctga tgatcattgg caccagcttt ttgacccgcg acgacgccag tttcgtcaaa
    12181 atggtcttta cgctggataa ctacacgcgt ctgctcgatc cgctctattt tgaagtgcta
    12241 ttgcactcgc tgaatatggc gctgatcgcc accctcgcct gcctggtgct gggctatcct
    12301 tttgcctggt ttctggcgaa gctgccacac aaggtgcgtc cgctgctgct gtttctgctg
    12361 attgttccgt tctggaccaa ctcattgatt cgtatttacg ggctgaaaat tttcctcagc
    12421 accaaaggct atctcaacga gtttttgctc tggctgggcg ttatcgatac accaattcgc
    12481 atcatgttca cgcctagtgc ggtgattatc ggtctggttt acattctgct gccgtttatg
    12541 gtgatgccgc tgtactccag tatcgaaaaa ctggataagc cgttgcttga ggcggcgcgc
    12601 gatctcggtg ccagcaagtt gcagaccttt atccgcatca ttatcccact aacgatgccg
    12661 ggaattattg ccggatgtct gctggtgatg ctgccagcga tgggcctgtt ctatgtgtcc
    12721 gacctgatgg gcggtgcgaa aaacctgctg atcggtaacg tcatcaaggt acagttcctc
    12781 aatattcgtg actggccgtt tggtgcagcc accagcatta cgctgactat cgtaatgggc
    12841 ctgatgttgc tggtttactg gcgcgcttct cgtctgctga ataagaaggt ggaactcgaa
    12901 tgatcggtcg actgcttcgc ggcggtttta tgaccgctat ctacgcgtac ctgtatatcc
    12961 caatcattat tttgattgtg aactccttta acagctcgcg ctttggcatc aactggcagg
    13021 gttttaccac caaatggtat agcctgctga tgaacaacga cagcctgtta caggcagcgc
    13081 agcattcact aacaatggcg gtgttttcgg cgacgtttgc tacgcttatc ggttcactga
    13141 cagcagttgc gctgtaccgt tatcgctttc gtggtaagcc gttcgttagc ggaatgctgt
    13201 ttgtggtgat gatgtcacca gatatcgtga tggcgatttc gctgctggtg ctgtttatgc
    13261 tgctgggtat tcagcttggc ttctggtcgc tgctgttctc gcatatcacc ttctgcctgc
    13321 catttgtggt ggtgacagtg tattcgcgcc tgaaaggttt tgacgtgcga atgctggaag
    13381 cggcgaaaga tctcggtgcc agcgaattta ccattctgcg aaaaatcatt ctgcctctgg
    13441 caatgccagc ggtagcggcg ggctgggtgt taagctttac cctgtcgatg gacgacgtgg
    13501 tggtttcttc gtttgtcacc gggccgagtt atgaaattct gccgttaaaa atttattcga
    13561 tggtcaaagt cggcgtatcg ccggaagtta acgcgctggc aaccatatta ctggtgctgt
    13621 cgctggtgat ggtaattgcc agccagctta ttgctcgtga taaaacgaaa ggtaacacag
    13681 gggacgttaa atgaaaaaat ggtcacgcca cctgctcgcg gcgggtgctc tggcactggg
    13741 catgagcgcc gctcacgccg atgacaacaa cacgctgtat ttctacaact ggaccgagta
    13801 cgtgccgcca ggactgcttg aacagttcac caaagaaacc ggtattaagg ttatctattc
    13861 gacttacgag tcgaacgaaa ccatgtacgc gaagctgaaa acgtacaaag acggtgccta
    13921 tgacctggtg gttccttcaa cctattacgt cgataaaatg cgtaaagaag ggatgatcca
    13981 gaagatcgac aagtcgaagt taagcaattt cagcaacctc gatccagaca tgctcaacaa
    14041 gccgtttgac ccgaataacg actattccat tccgtatatc tggggtgcaa cggcgattgg
    14101 cgttaacggt gatgcggtgg atccgaaatc tgtcaccagc tgggccgatc tgtggaagcc
    14161 tgagtacaaa ggcagcctgc tgctgaccga cgatgcccgt gaagtgttcc agatggcgct
    14221 gcgtaagctg ggctactccg gtaacaccac cgatccgaaa gagattgaag ctgcatacaa
    14281 cgagctgaaa aaactgatgc caaacgtggc ggcgtttaac tccgataacc ccgctaaccc
    14341 gtatatggaa ggcgaagtta acctcggcat gatctggaac ggttctgctt tcgttgcacg
    14401 ccaggcgggt acgccaattg atgtggtgtg gccgaaagaa ggcggcattt tctggatgga
    14461 cagcctggcg atcccggcaa atgccaaaaa caaagaaggc gcactgaaat tgatcaactt
    14521 cctgctgcgc ccggatgtgg caaaacaggt tgctgaaact atcggttatc caacgccaaa
    14581 ccttgcggcg cgtaagctgt taagtccgga agtggcgaac gataaaacgc tctacccgga
    14641 tgctgaaacc attaaaaatg gtgaatggca gaatgacgtt ggcgcagcca gcagcattta
    14701 tgaagagtat tatcagaagc tgaaagcagg acgttaattc agcaagcggg cggtggtatt
    14761 accgcccgtt ttttatttag caaggatgtg gctaatgagc caggacagta gagttttctt
    14821 tcgtatattc ttcggtatcg gtctggtact gatcctcatt tctgttgtta tcttttataa
    14881 ccagttcacc tatagcaaag acgcgattca tactgaaggc gtcattgttg ataccgtctg
    14941 gcactccagt cattctcatc gcacgggtaa aaatggttcg tggtatcccg tcgttgcctt
    15001 tcgtccgacg ccagattaca cgctaatttt caattcaagt attggcagcg atttttatga
    15061 agacagtgaa ggcgataaag ttaatgttta ttattctcca ggacatcctg aaaaggctga
    15121 gataaataac ccgtgggtta atttttttaa atgggggttt atcggcatta tgggcgttat
    15181 ttttatctcc gtcggtttat taatcagtat gccctactct aaaaagacac gcaggaaacg
    15241 gaagtcccgc ccatgaaaaa aatcataata ttgctcagtt tactgatact cctgcctctg
    15301 acggtaacca gtaagccatt gattccaata atgaagactc tttttacgga tgttaccggt
    15361 actgtaccgg atgccgaaga gatcgcgcgt aaagccgaac tttttcgtca acaaaccggg
    15421 attgcgccgt ttatcgtggt tgtacctggc gttaatgaag caagcttcag gcaaaatggc
    15481 aaagcgatgc tggcgcatgc ggcatcttca atgagtaatg taaaaggaag tgttctgtta
    15541 ctttttacta cccgcgagcc acggttaatt atgataacca acggccaggt tgaaagcagc
    15601 atggacgata aacatctcgg ccttctggta gaaaatcaca ctctggctta tttacatgca
    15661 gatctctggt atcagggaat caataatgca ttggctgttt tacaagcaca gatattaaaa
    15721 caaccgacgc cgccactgac atattatccg catcccggac aacagcagga gaatgatctt
    15781 cccggcagta ccacgacatt aggccttttt gcctgggcag ttgcattcat cgtctttgct
    15841 gcatttttta actacacaac gcgcctttat tatgcgttaa aatttgcagt ggctatggct
    15901 gttgccaata tgggctatca ggctttatgt ttgtatatcg ataacagctt tgcgattacc
    15961 agaatatcgc cgctctgggc agggctgatt ggtgtctgta cgtttattgc cgcgttacta
    16021 tggacgagta aacggtaaaa gaggcagtaa agttgccgga tgcggcgcga acgccttatc
    16081 cggcccacaa aacccgcaaa ttcaattaat tgcgcccctt cacaggcctg acaagcgtag
    16141 cgcatcaggc aatttttccc gctttataat cccttcagca acttttcaac aaactctggc
    16201 accacctggc ttgccggacc gtaatatttc tcggcaaatt cattaccaac ctgactcggt
    16261 tcaagattca gttccacggt gtgcgcgcca tgcagtttcg cttcgtgaac aaacccagcc
    16321 gccggataaa catgccccga agtaccaata gcaatgaaaa tatcggccat cgacaacgcc
    16381 atataaattt catccatgcc gagtggcatt tcgccaaacc acactacgtg cgggcgcaag
    16441 ggtgccggga actggcaaca atggcattta tcttctgggg taacatctcc ggtccagtcg
    16501 agaacctgac cactttgcga acagcgaact ttcagcagtt ccccatgcat atgaatgaca
    16561 ttggtattac ctgcgcgttc atgcaggttg tcgatattct gcgtcaccag caaaaagcga
    16621 tcgccgaggg cgtcttgcag tttcgccagc gcaagatgcg cggcgttagg ctgaatttct
    16681 ggctgctgca gctgtcgacg acgggcgtta taaaacgttt gcaccagttc aggatcgcga
    16741 tcgaaacctt ccggcgttgc cacatcttca acccgatgtt cttcccacag gccatctgcg
    16801 gcgcgaaagg tacgaatacc tgattccgca gaaatccctg cccctgtcag tacgagtact
    16861 cttggttttt ccattgcttc cggcaccact ttatctctga aaaaaatacg ctgacgcaaa
    16921 cgctcgcgca gacggcgttt atttttacga aaacgactta accgatgacc ccgacgcgac
    16981 agcatagcaa cctctgttgt ttaatcggtt agatgtagga aggccgcacc acgcattcct
    17041 cccgcatcac cgtggcgcgc gcgttcaatg cgcggaacac gagctacagg taagagatga
    17101 cgaggcagcc tgtccgccag ttgcgttgtg attgccggga aattcgataa gccaccacca
    17161 atgacgacca ggtcagggtc aacaatggtc aggatatttc ccagacaaac cgctaataaa
    17221 tccagataac gctcaacgtg cgcccttgcc tgctcatcac cttgatcata aagcgcaata
    17281 atttcgggag cctgcaacgg ttgatgataa tagtgttgat acagccacgc aaaaccgcga
    17341 ccagacagat aattttcaat gcagccatgc tgaccacagc cgcagcggcg taacgggaaa
    17401 tccagcccca tcatggttaa cgcatcaacc ggcagacgca tatggccaaa ctcgccggta
    17461 atatagcttt taccggtaat gggtttaccg ttgaaaatca gcccgccgcc aacgccggtg
    17521 ccgagaatca accccatcac cagtggatat tgagtaaatt catcatccca tgcttcagaa
    17581 agggcaaaac agttggcatc gttatcaagg cgtacatcgc gatcaagacg tgcgctcagg
    17641 tcggcacgca gcggtttacc gctggcagca gggacattgg cggcatacag cgtaccatct
    17701 tctgtttccg gcattcccgg aataccgatg ccgacagaac ctttacagcc aaaacgccgg
    17761 tcagcttcgg ctaccagctc gcacactgca tctaaaaatg cgtcatagct gtcacgcggt
    17821 gtcggcaccc gcttttccca ctgcaactgc cgaccgctat caaacacgcc aagcgcaatt
    17881 tttgttccac caatatcaaa cccgtaatac attgccgctc cttgttttaa tgtactgcct
    17941 ttactggccg ctaaggactc gcgcagggtc aatattactg gcgcgtcgcg ccggatacca
    18001 acttgccaaa agactcagca acaatgctgt gaccagtacg tagaagacgt ccagccagtg
    18061 caattccgat ggcaggaagt caataaaata gatatcgctg gagaggaact gatgaccgat
    18121 caacttttca atccactcaa taatcggggt aagttgcagt gaaacaacta cgccgataat
    18181 cacaccacac aggctaccga acagccctgc cagcaatcca taccagacaa agatggcgcg
    18241 aattaaacca tctttcgccc ccagcgttct taatactgcg atatcgccac tcttgtcttt
    18301 caccgccatc actaaggtgg agacgatgtt gaaacaggcc acgccaatca ccagtaccat
    18361 cgccagatac ataatggcgc ggatcatctg gatatcgcga tacatatagc cgtaagtacc
    18421 aatccagctt ttaatataaa catagctgtt ggtcacttca cccgcatcgc gtaccagctt
    18481 attggcgttg aaaacatccg tcattttaag ggcaatacct gacacgctgg aacccatatc
    18541 aagatattgc tgggcatccg ccagcgggat catggcaaaa ctgtgatcga gttgaccact
    18601 caactgcaaa ataccggcaa tgtgcaaacg cacacgtttt ggctgcatca gtttatgctc
    18661 gggattcgag ttggggatca taatcgacac ccaatcgccc tgcttcactt tcagcgcatc
    18721 cgccacgcct ttgccgatga taatttgctg ttcgcccgct ttaaaattgc gccaggcatc
    18781 cccctgaaca aacgagggta atgcgctcag acgctgttcc tgttgcgggt taacgccctt
    18841 cacctggatt gcgcgcagat tggcaccact ttctaccagt ccggtgaaat tgatatacgg
    18901 cgcagcggcg gcaatacctg gcactttttg cacgttatcc agggcttcct gccagttagt
    18961 ccacggttga tccaccgcct ctatttcgcc atgcggcacc accgccagaa tgcggttatt
    19021 cagttcgcgt tcaaagccgt tcatcgcgct taagccgacg atcaataccg ccacgccaag
    19081 ggcaatgcca atggtagaaa tgacggagat cagcgacacc atgccgccgc gccgccgtcc
    19141 gcggctaaaa cgcaggccaa tcaataacga taaaggcatc gccattactc cgcccccatc
    19201 aggctcagtt ccgccgtcag acgaccatca cgcatctcca gttggcggct catacgtttt
    19261 gccagttgca ggtcgtgagt aaccaccagg aaggcggtgc cctgcaagcg attcaattct
    19321 ccaagcaact ggaagatgct gtcggcgtta cgtgcatcga ggttaccagt aggttcatcc
    19381 gccagcacca gccgcgggtt attgaccagc gcacgggcaa tcgccacgcg ctggcgttcg
    19441 ccgccagaaa gttcagatgg gcggtgattc gcacgatgct ccagccccac cgcttttaac
    19501 atctccagcg cacggctgtt gatttcagcg ggctttttct tgccaatcag cagcggcata
    19561 gccacgtttt ccagggcagt aaaatccggc agcaggtggt gaaactgata aataaagccc
    19621 agcttctggt tgcgcagttc tgctttcgcc gccgacgaca gtttgctcat cggctgaccg
    19681 ttaaagatca catcgccgga ggttggcgta tccagcccgc ctaacaggtg cagcaaggta
    19741 cttttaccgg aaccagagct accgacaatt gccatcattt caccttcgcc cacgctgaaa
    19801 ctgacattgt gcagcacatc ggtttgcaca ctgccttcct gatagcgttt gcacaggttg
    19861 tcgcattgca acaggatctt attcataacg taaagcctca gcgggttgtg tggcggcagc
    19921 gcgccatgaa gggtaaagcg tagacagcag cgcgatagcc atcgccacca gcgcaataac
    19981 aatgacctgt aaaggttcga tagcgaccgg cagcgccgcg ccatcaagca ggacgccgat
    20041 tatcggcatc agattattta actggctggc aagcagtgcg ccaagcgccg ctccgaggat
    20101 cgcaccgata atcccggcgc tggccccttg caccataaag accatcatga tttgtcgcgg
    20161 agttaagcct tgcgtttgca ggatcgcgac ttcgccctgc ttctccatca ccattagccc
    20221 tagcgaggta ataatgttaa acgccgctac ggcaacaatc aggctcagca gcaagcccat
    20281 catatttttt tccatgcgta cggcctggaa cagctcgcct ttacggtcac gccagtcctg
    20341 ccatttgctg ccttcaggca gtttttgttg acttaatgag tcgactttca gcggctcatc
    20401 cagccacaaa cgccagccgg taatattgcc tgccggataa cgcatcagac gcgaggcatc
    20461 ctcaatattc accagcattt catagccatc gacttcactg ttggcggcga aagtgccaat
    20521 cacattgaac aggcgctggc ttggaatacg ccccatcggc gtgaactggc tggcagatgg
    20581 caccatcacg cggatttgat caccgcgatt gacgcccaac tgtgaggcca gctgttcacc
    20641 gaggatgaca ttatatttcc ccggctcgag gtcagtttgt ttcacattga ccagatacgg
    20701 cgttagtgga tctttttgtg ccgggtcgat acccagcatc accccgaccg ccacgctgcg
    20761 cgcgctttgc agtaccacat caccggtagt aataggtgcg acgcgattaa cgccgtccag
    20821 tttgactgcc gtttccggga gttgctgcgg gttaagagag ccatgctcag aagagagaat
    20881 tgcctgtggc atcaggccaa ggatgttgtt ttgtagctcg cgctcaaagc cgttcatcac
    20941 tgacaatact gtgaccagcg ccatcacccc gagggtaatg ccgatggtag aaagccagga
    21001 gacgaaacga ccgaagcgat ccgctgcacg cccacgcatg taacgcaggc caataaatag
    21061 agcgacaggt tggtacatga aatccgtctg gttgctgtta gcaaagacac gagtatataa
    21121 gcgaaagcgg gatgggtaaa cgtctgaact gtaagtgatc gtcagggcta atctgaaaag
    21181 tgccagcaaa tcaatcggct aaagggcctc attttctcat cggccttgta gcctgatctt
    21241 tatctgaaaa ataaccaaat acagacagat acgcattaca ccacgcgact ggcccctcag
    21301 gatcaacagc atgttaaata aaaagagcgt gttgtcctga tgaaacaaaa agagctatgg
    21361 attaaccaga tcaaagggtt atgtatttgt ctggtggtga tttatcactc ggtcattacc
    21421 ttttatccgc atctcaccac ttttcagcat ccgttatcgg aagtcctgag caaatgctgg
    21481 atctatttca atctttacct tgcacccttt cgtatgccgg tttttttctt tatatccggc
    21541 tatttgattc gccgttatat cgacagcgta ccgtggggaa attgtctcga taaacggatc
    21601 tggaacatct tctgggtgct ggcgctttgg ggcgtggtgc agtggctggc gttaagtgcg
    21661 ctgaaccagt ggctggcacc tgagcgcgat ttaagcaatg cctccaatgc cgcgtatgcc
    21721 gattccaccg gtgagttcct gcacgggatg atcaccgcca gcaccagttt gtggtatctg
    21781 tatgcgttaa ttgtttattt cgtggtatgt aaaattttta gccgtctggc gctgccacta
    21841 ttcgccttgt ttgtactgat gagtgtggcg gttaatttcg ttcccacgcc gtggtgggga
    21901 atgaacagtg tgatccgcaa tttgccttat tacagccttg gcgcatggtt tggcgcaaca
    21961 ttaatgacct acgttaaaga ggtgccgttg cgccgccatc tgctgatgac ttctttgctg
    22021 accgttctgg cggtcggtgc ctggttgttt actatctcgc tgctgttgtc gctggtatcg
    22081 attgtggtga tcatgaagct gttttatcaa tacgagcaac gtttcggtat gcgctccacc
    22141 agcctgctga atgtgattgg caccaacacc attgctatct acaccaccca tcgcattctg
    22201 gtagagatat tcagcttaac tctgcttgcg ccaatgaacg cggcacgctg gtcgccgcaa
    22261 gtcgaactga cactcctgat ggtttatccc tttgtcagtt tgttcatctg tactgttgcg
    22321 ggcttgctgg taagaaaact ttcacagcgt gcattcagcg atctgttgtt ctccccgcct
    22381 tcactgcccg ctgccgtcag ttactcccgc taagccatcg ccccagccgg ggcgattcaa
    22441 tttgctaaac catgtcgatt acggataatg aacagcattg cttatcagag atatgccccc
    22501 atatgttgag gcatatccta acgagaatct gacaaccgtt atgcctgaac aatatcgtta
    22561 tacgctgccc gtcaaagcgg gtgagcagcg tctgctgggc gagttaaccg gcgctgcctg
    22621 cgcgacgctg gtagcggaaa ttgccgaacg tcacgccggt ccggtggtac ttattgcacc
    22681 agatatgcaa aatgctctgc gtttgcatga tgaaatcagc cagtttaccg atcagatggt
    22741 gatgaatctg gcggactggg aaacccttcc ctacgacagt ttttcgcctc atcaggacat
    22801 tatctcctcg cgcctttcca ccctttacca gctaccgacc atgcagcgtg gcgtactgat
    22861 tgttccggtg aatacgctta tgcagcgcgt ttgcccgcac agttttctcc acggtcatgc
    22921 gctggtgatg aaaaaaggtc agcgcctgtc acgagatgca ttacgaaccc aactggacag
    22981 cgccggttat cgccatgttg accaggtaat ggagcacggc gaatacgcca cgcgcggcgc
    23041 gttgctggat ctcttcccga tgggcagtga gctgccttat cgtcttgatt tctttgatga
    23101 tgaaatcgac agcctgcgag tgtttgacgt cgatagccag cgcacgctgg aggaagtaga
    23161 agagatcaat ctgctgcccg cgcacgaatt tccgaccgat aaagcggcaa ttgaactgtt
    23221 tcgcagccag tggcgcgata ccttcgaagt gaagcgcgat ccggaacata tttatcagca
    23281 agtgagtaaa ggcacattac ctgccgggat cgagtactgg cagccgctat tcttcagcga
    23341 accgctgccg ccactgttca gttatttccc tgccaatacg ctgttggtga atactggcga
    23401 tctggaaaac agtgccgaac gtttccaggc tgacacgctg gcgcgttttg aaaatcgcgg
    23461 cgtcgatccg atgcgcccac tgttgccacc acaatcgctc tggctgcggg tagacgagct
    23521 cttttcagag ctgaaaaact ggccgcgtgt gcagctaaaa actgaacatt taccgacaaa
    23581 agccgcgaat gccaatttag gtttccagaa actgccagac ctggccgttc aggcacaaca
    23641 aaaagcaccg ctggatgcgc tgcgtaagtt cctcgagtct ttcgacggtc cggtggtgtt
    23701 ctcggtagaa agtgaaggtc gccgtgaagc gctgggtgaa ctgctcgcgc gaattaaaat
    23761 tgctccgcaa cgcattatgc gtcttgatga agccagcgac cgtgggcgtt atttgatgat
    23821 tggcgctgcc gaacatggtt ttgtcgatac ggtacgtaat ctggcgctga tttgcgaaag
    23881 cgatctgctc ggtgaacgcg ttgcccgtcg tcgtcaggat tctcgccgcg ccatcaaccc
    23941 cgatacactg atccgtaacc tcgcggagct gcatattggt cagccggtgg tccatctgga
    24001 gcacggtgtc gggcgctatg ccggaatgac cacgctggaa gcgggcggta ttactggcga
    24061 gtacctgatg ctcacctatg ccaacgacgc caaactgtat gttccggtgt cgtcactgca
    24121 tctgattagc cgttacgcgg gtggcgcgga agaaaacgcc ccgctgcata aacttggcgg
    24181 cgatgcctgg tcacgcgcgc ggcagaaagc ggcggaaaaa gtgcgtgatg tggcagcaga
    24241 attgctggat atctacgcgc aacgcgccgc caaagagggc ttcgcgttta aacacgatcg
    24301 cgagcagtat cagttgttct gcgacagctt cccgtttgaa accacgccgg atcaggcaca
    24361 ggccattaat gcggtactta gcgacatgtg tcagccgctg gcaatggatc gtctggtgtg
    24421 cggcgatgtt ggctttggta aaacagaagt ggcgatgcgc gccgctttcc tggcagtaga
    24481 taaccacaag caggtggcgg tgctggtgcc aaccaccctt ctcgcgcagc agcattacga
    24541 caacttccgc gaccgtttcg ccaactggcc ggtacgtatc gaaatgatct cccgtttccg
    24601 cagcgccaaa gagcagacgc aaatccttgc ggaagtagcg gaagggaaaa tcgatattct
    24661 gatcggtacg cacaaactgc tgcaaagtga cgtcaagttt aaagatttag gcctgctgat
    24721 tgtcgatgaa gaacaccgct tcggggtgcg tcataaagag cgcattaaag cgatgcgcgc
    24781 gaacgtggat attctgacgc ttactgcaac gccgatccca cgtacgctga atatggcaat
    24841 gagcggaatg cgtgacctgt cgattatcgc cacgccgccc gcccgtcgtc tggcagttaa
    24901 aacctttgtc cgtgagtatg acagtctggt ggtccgggag gcgatcctgc gtgaaatttt
    24961 gcgcggagga caggtttatt atctctacaa tgatgtggaa aacatccaga aagccgccga
    25021 acggctggca gaactggtac ctgaagcgcg gattgccatc ggtcacgggc aaatgcgtga
    25081 gcgcgaactg gaacgggtga tgaatgattt ccatcatcaa cgtttcaacg tgctggtttg
    25141 taccaccatt atcgaaaccg ggatcgacat tcctaccgcc aacaccatta tcattgaacg
    25201 cgcggatcac ttcggtctgg cacagttgca ccagttacgc ggtcgcgtcg ggcgttcgca
    25261 ccaccaggca tatgcatggc tgctgacgcc gcatccaaaa gcgatgacta ccgatgcgca
    25321 aaaacgtctt gaagcgattg cctcgctgga agatctcggg gcaggttttg cgctggcaac
    25381 gcacgatctg gagatccgcg gcgcaggtga actgcttggc gaagaacaaa gcggctcaat
    25441 ggaaaccatc ggtttctcgc tgtatatgga gttgctggaa aacgctgtcg atgcgctgaa
    25501 agccggacgc gagccgtcgc tggaagatct caccagtcag caaacagaag tcgagctgcg
    25561 gatgccgtcg ctattgcccg atgatttcat ccctgacgtg aacacgcgtc tgtcgttcta
    25621 caaacgtatt gccagcgcca aaacggaaaa cgaactggaa gagatcaaag tcgagcttat
    25681 cgatcgcttc ggcctgctgc cggatccggc gcgtaccctg ctggatattg cccgtctgcg
    25741 ccagcaagcg cagaaactgg ggatcaggaa gctggaaggt aatgagaaag gcggcgtgat
    25801 cgaatttgcc gagaagaatc acgttaatcc ggcctggttg attggtttgc tgcaaaaaca
    25861 gccgcagcat tatcgcctcg atggtccgac gcgcctgaag tttattcagg atttgagtga
    25921 gcggaaaacg cgtatcgaat gggtacgcca gtttatgcgt gaactggaag agaacgcgat
    25981 cgcttaatgg taaatcgggg gcgtttctgc gcccccatct gcaacattta caaattcttt
    26041 gcacttccct gcactatccg acactgtcac catccataat tcaggcttat ctgtttatta
    26101 caataacctt atatttatta tggatttttg gtgatgatca aaacgcgttt ttctcgctgg
    26161 ctaacgtttt ttacgttcgc cgctgccgtg gcgctggcgc taccggcaaa agccaacacc
    26221 tggccgctgc cgccagcggg cagtcgtctg gttggcgaaa acaaatttca tgtggtggaa
    26281 aatgacggtg gttctctgga agccatcgcc aaaaaatata acgtcggctt tctcgctctg
    26341 ttacaggcta accccggcgt tgatccttac gtaccgcgcg cgggcagcgt gttaacgatc
    26401 ccgttgcaaa ccctacttcc agatgcgccg cgcgaaggca ttgtgatcaa tattgcggaa
    26461 ctgcgtctct attactaccc gccgggtaaa aattcggtaa ccgtgtatcc catcggcatt
    26521 ggtcagttag gtggtgacac gctgacgccg acaatggtga ccaccgtttc agacaaacgt
    26581 gcaaacccaa cctggacgcc aacggcaaac atccgcgccc gttataaagc acagggaatt
    26641 gagttgcctg cggtagttcc ggctggaccg gataatccaa tgggccatca tgcgattcgc
    26701 ctggcggcct atggcggcgt ttatttgctc cacggtacga acgccgattt cggcattggc
    26761 atgcgggtaa gttctggctg tattcgtctg cgggatgacg atatcaaaac actctttagc
    26821 caggtcaccc caggcaccaa agtgaatatc atcaacactc cgataaaagt ctctgccgaa
    26881 ccaaacggtg cgcgtctggt tgaagtacat cagccgctgt cagagaagat tgatgacgat
    26941 ccgcagctgc tgccaattac gctgaatagc gcaatgcaat catttaaaga tgcagcacaa
    27001 actgacgctg aagtgatgca acatgtgatg gatgtccgtt ccgggatgcc ggtggatgtc
    27061 cgccgtcatc aagtgagccc acaaactctg taaagctcgg ataaaaaaat ccccgcgaca
    27121 cggcatgtcg cggggaaaaa aatcagcagt agcaggcatt aatgagggtt aatgcttatt
    27181 tataaattac tgctgttcca tggagggtat tcggaccggt tacagaagta atacggaaag
    27241 attttgcgcc catctcatcc gctttttgcg ccagctgctc ttccagcgat cccagatttg
    27301 tccccgcgtt agcactgata gtaccgactt tttgttggcc ttctggcgtt gactgaactt
    27361 cgacagccgc aaagctggca aatgacattg agcttaaaat cgccgcagcg atgagggttt
    27421 ttacgttttt cataatagtg gccttatgca gatgaatgac gtcgcaagca attacttaac
    27481 gatcgataac taaatgatag atgtgatctg gatcacatac aagatatttt ttataacaat
    27541 cattaattaa tttaaaaatc tttaaatttc agttaaatag aaagcaatta tttttatggt
    27601 ttgtggactg gtgaacggag gtgattattt ttataataac ctttcattca cagtcaccag
    27661 gtacaacggc atcatggcaa ctgactcaac acaatgtgta aaaaaaagcc gcggccgccc
    27721 aaaagtgttc gacagggatg ccgcgcttga taaggccatg aaattgttct ggcaacacgg
    27781 ttatgaagcg acttctcttg cggacctcgt cgaagcgacc ggagccaaag cgcccacgct
    27841 atacgcggaa tttaccaaca aagaggggtt attccgcgcc gtactcgacc gctatatcga
    27901 tcgttttgcc gctaagcatg aagcacagct gttttgtgaa gagaaaagcg tggagtctgc
    27961 gctggctgac tattttgctg ccatcgccaa ctgctttacc agcaaagaca ccccggcagg
    28021 ctgcttcatg atcaacaact gcaccaccct ctccccagat tcaggagata tcgccaatac
    28081 gctgaaatca cgccatgcga tgcaagagcg cactttgcag cagtttttat gtcaacgaca
    28141 agcgcgcggg gaaatcccga cccactgtga cgtgacgcat ctggcagaat tccttaattg
    28201 tattattcag gggatgtcga tcagcgcacg cgaaggtgca tcgctggaaa aactgatgca
    28261 gattgcccga acgactttgc gtttatggcc cgaactggtg aaataaggcg caaaaaaagc
    28321 ccctcagcct gagcgaatga ggggccaaat tgcagagtac agccttgtta tactttgtta
    28381 ttcaaaatca gttgcccgtt gctatctagc gggatctgcg tacccggatc gcggtccatg
    28441 cggattttgc cctgctgatc gccaatctta taggtcacat cataaccgag cattttttct
    28501 gacttgtcat acaccgtttt acaacgctgt tgcgtagtcg tgtaagtatc gctttcctgg
    28561 agagagccct ggatctggtt accggcatat ccaccaccca gcgcccccac aacagtagcg
    28621 acatctttac cgcgaccacc accaaactga tgccctatca cgccgccagc aacagcgccg
    28681 agcaccgacc cggtaatgcg attttcatcc tgcaccggtc gacgatgggt cactgtgacg
    28741 ttacgacact cctgacgcgg tgttttaacc gtttccttga ttggggttgc agaaacaacc
    28801 tgagcgtatt gcgggccacg ttcaaacacg ttcagactgg ccactgccgc tacgcccaac
    28861 gcagctgcga caccaatccc gatacccgcc aacattgatt tattcacggg aatacctcct
    28921 tctttgcagt tatgccaatc atgcaagcga aggagaaaag cagctatcag acagaagatg
    28981 aaaagcgcgg gaatggctgg aaatacttag ggattcggag gagtctgaag gaatgcctga
    29041 tgcgacactt aacgcgtctt atcaggccta ccgctcgtac catttgtcca gggcggataa
    29101 ggcgtttatg ccccatccgc cagtacaagc cgattaatgc aacttcaaac gtggacggat
    29161 aacgcggtta atactcccca ccagcatcat taatccagtt ttaaagtaac catgcagcgc
    29221 aatctgatgc attcggtata gcgagatata tacaaagcgc gcaattcgtc cttcaatcat
    29281 cattgagccg cgtgtcaggt tacccatcag gctaccgacg gtggaaaagt tcgacagcga
    29341 taccagcgaa ccgtgatctt tatactgata atttttcagc ggcttaccgt tcatctgcgc
    29401 cagaatgttg ttcattgcgc aggttgccat ctgatgtgcg gcctgagcgc gcggcggaac
    29461 aaagccccct tccggacgcg ggcatgacgc gcagtcgcca atagcgtata tgtctggatc
    29521 gcgagtggtt tgcagcgtcg gttccaccac cagctggttg atacggttgg tttcaagacc
    29581 accgatatct ttcaggaagt ctggcgcttt gatcccggct gcccacacca tcagatcagc
    29641 ctcaatatat tcgccatctt tagtgtgcag gccgccttca tcagcactgg tgaccatggt
    29701 ttgcgtcagc acgcgaacgc caagtttcgt taactcacta tgggccgcag cagagatacg
    29761 tggcggcaat gcaggcaaaa tacgttcgcc cgcttctacc agcgttacgt tcagggcttc
    29821 gttggtcaga cctttataac cgtaactgtg cagttgcttg accgcgttgt gcaattcagc
    29881 ggagagttct acacccgtcg cgccgccgcc gacaatcgca atgtttactt tgccattcgc
    29941 gcccaggttg gcggagtatt tcaggaacag attcagcatc tcctggtgga agcgacgcgc
    30001 ctggtgcggg ttatcgagga aaatgcagtt ctctttgaca cctggcgtat tgaaatcgtt
    30061 ggaggtgcta cccagcgcca ttaccagggt gtcataggcg attttacgtt ccggaaccag
    30121 caattcacct ttttcgtcgc gcagttctgc aatagtgatt gttttcgctt cacgatcaat
    30181 atcaattacg gaacccagct ggaactggaa accatgattg cgcgcatggg ccagatagct
    30241 caacgcatcg acgccttcat caagcgagcc agtcgccact tcgtgcagca gcggtttcca
    30301 caggtggctg tggttacgat cgaccagcgt aattttggct tttttcttgc gtcccagctt
    30361 atgccccagc tgtgttgcca tttccagccc accagcaccg ccgccgacaa tcacaatttt
    30421 tttcaatggc gtagtcaacg tgaccccctt aaatttatta accaattgtt aattaatggt
    30481 tattaacata gctcttaatt aacaacaggt tacagcaatg aaaatgttct tcactattga
    30541 gaataacatg aatggtgcat tggtcatacc aaaattgatg ttaatcaagt tttgttgctg
    30601 aaaagatagg cgactttacc gggcgagatc aaaaaacaga gtttattgtt tattatttga
    30661 gcgaatttgt gagagtgata tagagtgagg ggtgtatgga gtgcgttcgg taggcctgat
    30721 aagaggcgca tcaggcattg atgctcgact gtcggatgcg gcgtggacgc cttatccgac
    30781 ttacagaatt cggagcgcat caggtaccgg caaggttacc tgatgcgacg agggcattta
    30841 cccgagggtt ttgaacgcct taatgcgctg caaatgcggg gagatattct tgaatttgtg
    30901 cgtctgctct tcgtcccaga caatctcgta ataatgatgc agctcttcag atgtccgctg
    30961 gctgttaaga gcttcatcat tacgcgacaa aatcaccaaa caacgatcgc gattcttttc
    31021 gcggaagttg gtcacacact tagtggcaat atcggcatac tcttccgggc gatcaatctt
    31081 gccttccatg ttctcataag ggaacaaatt agggttgaag atcacctggc ggatatcgca
    31141 aagaaaacca atccgttccg cccagtatcc gcccagacca acgccgcaaa tcagcggacg
    31201 ctcgtcgacg ttcagttgca acattttgtc cacttctttg agcagatgct gcatatcatg
    31261 tttcggatgc cgcgtactgt agcttatcaa gcgtacatcc gggtcaataa actgcaattg
    31321 taagactttc tcgtggttac ccggactgtt agagtcaaaa ccgtgtaaat agataatcat
    31381 cgcttcctca cataagccag ggttagtgac ctgctttctc ttcctgccag cgttcatgta
    31441 actgattcag acgggcgctg atcgctttcc agcgagccga gtccatcagt tcctgtcgcg
    31501 aaaatgaacc tttatgatac aaacgtgtaa cacgttctgc cttgatcggt gacagattat
    31561 ctaacacgct gacggcccct ttacgattat tgcagaccag gatcatatcg caacccgcgt
    31621 ccagtgatgc ctgcccgcgt tcggcataac tgcccataat cgcggctcct tccatcgata
    31681 aatcgtcaga gaaaatcacg ccatcaaaac ccagttcctg acgcaaaacg gttttcagcc
    31741 aatagggaga accgctcgcc ggacgcggat cgacatcact gtagatcaca tgcgcaggca
    31801 taatggcgtc gagtttattt tcgcggatta aggaactgaa gaccgacata tctttagcgc
    31861 gaatctccgc ttgcggacgt gggtcgcacg gtgtttcttt gtgtgagtct gccgttactg
    31921 caccgtgtcc tgggaagtgt ttcccggtcg ttttcattcc ggcttcatgc ataccatcaa
    31981 taaaccggct ggcaattgcc agggcttttt cgggatcgga atgataagaa cgctcgccaa
    32041 tcgccgcgct gatatgcccg acgtccagca ctggcgcaaa gctgatatca atatccatag
    32101 caatcatttc gctggccatc aaccaacctg cctctcgcgc cagttttccg ccttcttcca
    32161 ttcccaacag cgcagcaaat gattgtgccg ctggcaagcg ggtaaaacct tcacgaaaac
    32221 gctgcacgcg tccaccttcc tgatcaaccg ccaccaccag atgattgcgc gaagctgcgc
    32281 ggatctggcg caccagttca cgtaactggg caggatcatg atagttacgc gtaaagagaa
    32341 tcagccctcc caccagcgga tgcgccagta tttcacgctc ttccgcgtcc agttcgtaac
    32401 cttcgacatc caacattact ggacccacac tgctctcctt attgttttat taatagctgc
    32461 cgccaggtgt catcggccag cctgataaat tgttgatcgc cggtttgtcg ccagcggtac
    32521 tcaaaccacc ctgctttgag catcagcagc cagggaaacc atcgcctgac ctgacgccat
    32581 aattgcgccg gataaatctt cgcgcgagtg gcatagtcat tgaccaattg ccggtgctgg
    32641 tcagtatttt ccacccacac cgccgccagt tccagcgcga tatcaccatc tccggcatac
    32701 tcccagtcga tgagttttaa ccctgacgcg ctatgcacta aatttccggc gtggacatcc
    32761 atatgcaatg gacttaagcg taaaggccgt ggttcccgcg ctttgcgcag acgttttaac
    32821 cttcgcagcc aacccactgt ccgccgcgcc ggatcgcttt gctgccagta cagttccagt
    32881 aacggcaaca gcgttattcg ccagccaaaa cgcggttgtt gatgtagata atacagcaag
    32941 cctgccagtt cgttggtatc cggcaaatac gtttttacct cgccgggcag atagtcgacc
    33001 accatccagt cacggagata taaatgcggc ttcggtgcaa tgcatgcggg tagttgtgat
    33061 aaagcccgat actggcgcaa gaacgcgaac cgcggcgcat caggatcgtg cggctgacgc
    33121 acaacaaaac gctggccctg atgttcaatg agaaaactcc cgccactaag cccactatta
    33181 aacgtcgtga cgggatgaaa ctgcgggaaa aagcgcgaca gcaattcgtc gcgcgtgatg
    33241 ggattattgc tgcgaaacgg cacctttacc tgaccagata atttcgcccg tctgcaccag
    33301 catcagctgc atttgaaggg tcggagcgtt aacattgcca gaggcgctgg agtacagcac
    33361 gtaatgagcg ccgacattgc gggcaatgcc tatggcttta ctacgggtgc ctaaactgtc
    33421 ctgcggcgac aaacctaact gttgctttgc catcgacagc tgctgggcgg aaaccagggt
    33481 aaatttaccg ttattagcca gcgcatttcg cagcgtttcg gtcgcttctg cggcattcag
    33541 cgaaccgtta gtacggttgt taacgctatc aaccagcagg acgctacccg cagtcacgcc
    33601 gtcagcccca agcatcttac tgaccatcgg ctgcattgcg ccattccagt cataatggcg
    33661 aatatgcggc gcaggcggtg cagtttgatc ttcgtgctca attgggcctg gctgctgcgg
    33721 tatcgtcggc accgatggca ctgtggggac aggctgttgc ggctcggctg gttgttccgg
    33781 cgctggtttc acttcttcta ccggcgcagg ttcacgttgc cccacacacc cggcgagaaa
    33841 catcgccagc gcggtaatca aggcgtagcg actcattttg gtcatcaaga ttcacccctt
    33901 acaaatatag ataaagtctg actttgtgcg cccccagaaa attggcgctg ccgtacagcg
    33961 ttaccgccga atgtgcggga agggtaacgc tgcgtggcct ttccagagga tgcatctcca
    34021 gccctctggc gtcataccag taaaaacgat aatgaacggt aacgggttct tgcctttcgt
    34081 tataaagcgt tgaggatgct gaaggttgaa tatcagacgt cgaaaggacg ggcttttctg
    34141 cactgatgcc cgcagccaat aaagatgact ccatcaccag cgattgctca tcattcaccg
    34201 gaatttccgg atgtgaacga cagcccacca gcactaacaa cgccagagac accagcccaa
    34261 agcatccttt tctcattgtt acagaccttt atgcgccagc attggtccca gtggacggcc
    34321 acccaacaaa tgcatatgga tgtggtaaac ctcttgtccg ccatggcggt tggtgttcat
    34381 gatcaggcga tagccatctt cggcaatacc ttcttgctca gcaatttttg ccgctacggt
    34441 gatcatgcgt cccagcgcct gctcatgctc agctgacacg tcgttcacag tcggaatgag
    34501 gatattcgga atgatcagaa tatgcgttgg cgcttgcggc gaaatatcgc gaaacgccgt
    34561 taccagatca tcctggtaga cgatatcgga ggtgatctca cgacgaataa ttttgctgaa
    34621 tatagtttct tctgccacga cattttcctt tttcaaatag ccatgcgttg cgccatgcta
    34681 cgccgcgcca cactgcgagt atagagtatg gtcgagttaa acctgctctt tcaaccttaa
    34741 ctcacgattt cttaagcaaa aaatcgtttt gctgccgtat atatcgccat tattcccatt
    34801 tctgcctgcg aatgcgctga aaagacattc tctgtttggt ttacacgtca aatatgaatg
    34861 cgtatatttc tcatttgcat ttacatgcaa aattattcgc acactaaata taaccgctca
    34921 tcaccctcct ccggcatgag cctgacgaca acataaacca agagatttca gatgctttca
    34981 acacaattta acagggataa tcaatatcaa accacctcca aaccgtcact gcttgccgcc
    35041 tgcatagcac tggcactctt accttccgcc gcttttgctg caccagccac tgaagaaacg
    35101 gtgattgttg agggttcagc cactactcca gttgatgacg aaaatgatta cagcgtaacg
    35161 tctacctctg cgggtaccaa aatgcagatg actcaacgtg atattcctca gtcggtcact
    35221 attgttagtc agcagcggat ggaagatcag cagttacaaa cgctgggcga agtgatggaa
    35281 aacacgctgg ggatcagcaa aagccaagcg gattccgatc gtgctcttta ttattcccgc
    35341 ggattccaga tcgataacta tatggttgat ggtatcccca cctattttga atcgcgctgg
    35401 aatctgggcg acgcactttc tgatatggca ctgtttgaac gcgtagaagt agtgcgtggc
    35461 gcgacaggac tcatgaccgg gacgggtaat ccatctgcgg caattaatat ggttcgaaaa
    35521 cacgcgacca gtcgtgaatt taaaggcgat gtctcggcgg aatacggtag ctggaacaaa
    35581 gaacggtatg tggcggattt acaaagccca ctcaccgaag atggtaaaat ccgcgcacga
    35641 attgtcggcg gctaccagaa taacgactca tggctggacc gctacaacag tgaaaagacc
    35701 ttcttctccg gcattgtcga tgctgattta ggcgatctta cgacgctttc agccggttac
    35761 gaatatcagc gcattgatgt taatagccca acctggggcg gtttaccgcg ctggaatact
    35821 gatggcagca gcaacagtta cgatcgcgca cgcagtaccg cacctgactg ggcgtataac
    35881 gataaagaga tcaacaaggt ctttatgacc ctcaagcagc ggtttgctga tacctggcaa
    35941 gcgacactga atgccaccca ctccgaagtc gaatttgaca gtaaaatgat gtatgtcgat
    36001 gcctatgtaa acaaagcgga tggtatgctg gttgggccat acagtaatta tggacctggc
    36061 tttgattatg tcggcggtac cggttggaac agcggcaaac gtaaagttga tgcgctggat
    36121 ttgtttgctg acggtagtta tgaattgttt ggtcgtcagc acaatctaat gtttggtggc
    36181 agttacagca aacaaaacaa tcgttacttc agttcatggg cctacatctt cccggatgaa
    36241 attggcagtt tctacaactt taatggcaat ttcccacaaa ccgactggtc accacagagc
    36301 ctggcgcagg acgataccac acatatgaaa tcgttatatg ctgccactcg tgtcaccctt
    36361 gccgatccgc tgcatctgat cctcggcgca cgttatacca actggcgggt tgatacgctg
    36421 acttacagca tggagaaaaa ccacaccacg ccttacgctg gtctggtgtt tgacatcaat
    36481 gacaactggt cgacctacgc cagctatacc tctattttcc agccgcaaaa tgatcgtgac
    36541 agttcaggca aatatctggc tccaatcacc ggtaacaact acgagctggg tctgaaatcg
    36601 gactggatga atagccgtct gaccaccacg ttagccatct tccgtattga gcaggataat
    36661 gtcgctcagt ccaccggtac acctatcccc ggcagcaacg gcgaaaccgc ctataaagcg
    36721 gtggatggga cagtcagtaa aggtgtggaa tttgaactca acggcgcaat taccgacaac
    36781 tggcagctga cgtttggcgc aacgcgctat attgcagagg ataacgaagg aaacgccgtt
    36841 aatcctaatc tgccacgcac cacggttaaa atgttcacca gctaccggtt gcctgtcatg
    36901 ccagagttga cggtcggcgg tggtgttaac tggcaaaatc gcgtgtatac cgacaccgtg
    36961 acaccgtatg gcaccttccg cgccgagcaa ggtagctacg cgctggtgga tctcttcacc
    37021 cgctaccagg tgacgaaaaa cttctcgtta caggggaatg tcaataacct gttcgacaaa
    37081 acctacgata ccaacgtgga aggttctatt gtctacggcg caccgcgtaa tttcagcatt
    37141 accggcacgt atcaattctg aataacacct gtaaaaaagg cagccatctg gctgccttag
    37201 tctccccaac gtcttacgga ttagtggtta cggatgtact catccatctc ggttttcagg
    37261 ttatcggatt tagtaccgaa aatcgcctga acaccagaac cagcaaccac tacgcccgct
    37321 gcgcccagtt tcttcaggcc agcctgatcc actttagaca catcagcaac gctgacgcgc
    37381 agacgggtaa tacatgcgtc gaggttagta atgttttctt taccaccaaa tgcagcaacc
    37441 agagccggtg ccatttcgct ggtacctgtc gcttttgcgt cttcagtcgc gtcttcacga
    37501 cccggcgttt tcagatccag tgctttaatc agcacgcgga agatggtgta gtaaacaatg
    37561 gcataaccga taccgacgat cgggaacagc cacagtttgc tgctgttacc agacagaacg
    37621 atgaagtcga tcagaccgtg cgagaacgac gtaccgtcac gcatccccag aagaatacag
    37681 attgggaatg ccaggcctgc cagaatcgcg tggatgatgt acaggatcgg cgcaacgaac
    37741 atgaaggaga actcgatcgg ctcggtgata ccggtcagga acgaggtcag cgccgcggag
    37801 atcataatac cgcccacttt cgcgcggttt tctggtttag cagagtgcca gatagcaatt
    37861 gcggcagctg gcagaccgta cattttgaac aggaagccac cagacagttt acccgcagtc
    37921 gggtcacccg ccatataacg cggaatgtcg ccgtggaaaa cctgacctgc tgcgttggtg
    37981 tattcaccaa tctgcatctg gaaaggtacg ttccagatgt ggtgcagacc aaacggtacc
    38041 aggcaacgtt cgatgaaacc gtaaatgcca aacgcaacta ccgggttctg gtaagcagcc
    38101 cactgagaga aggtctggat tgcagaacca atcggcggcc aaatgaagga cagcacaacg
    38161 ccagtaaaga tggcagccag gcctgaaatg atcggcacaa agcgtttacc ggcaaagaag
    38221 ccaagatact caggcagctt aatacggtag aaacggttaa acatgtacgc tgcgatcgca
    38281 ccggagataa tccccccgag tacgccagta tccgccaggt gtttagaggc gatttcttca
    38341 gcaggtaaat gcagtaccag tggcgcaacc acggccatgg ttttaaccat gatgccatag
    38401 gcaacaactg cggccagcgc ggatacgcca tcgttattgg taaagccgag ggcgacaccg
    38461 atcgcaaaaa tcagtggcat gtttgcaaag acggaaccgc ctgcttctgc cataacatgc
    38521 gatacaacgg cgggcagcca gctgaaattc gcggaaccga cgcccagcag aatacctgcg
    38581 ataggcagta cggataccgg cagcatcagc gatttaccga ccttttgcag gttagcaaat
    38641 gcattcttaa acataattga gagtgctcct gagtatgggt gcttttttta cgttttcacg
    38701 cgtggcaagg ggggagagcc tcgccgtgta cagggcatct aagcgccctt tatttattac
    38761 acagagtaaa ataattcagt gccgatttgt ttgacggcta tcacgtttca acttcagatc
    38821 attggagaag ttgcacattt tgacaaaaca tatgtcacaa tatttcaaac gtggttattc
    38881 accgcccaat tttaggcggt gaactttact cgttttagcc attaattacg agctttaaaa
    38941 aaactcattc aacggatgga ttgaaggcgg gaagcgtcga tgtgaaacag acgggcgaag
    39001 ttatcggtgg ttacctgcgc cagttcttca acggcaacac ctttcaacac agccatgtat
    39061 tctgcaacgt cacgaaccat cgcaggttga ttctcttttc ctcgatgcgg aaccggtgca
    39121 aggtaaggtg agtcagtttc taccagtaac cgatcctggg ggacataacg cgcggcatcg
    39181 cgcagttgct ccgcattacg gaaggtcaca atgccggaaa aggagatgta aaatccgaga
    39241 tccagtaatt tacccgccgt ttctctgtcc tctgtaaaac agtgtagtac gccaccgcaa
    39301 tccgtcactt tttcttcgcg cagaattgcc agcgtatcgg cgcgggcgtc acgggtatgg
    39361 acgataaccg gcttgttcag ttcacgacca atctggatat gatggatgaa ggattcttgc
    39421 tgccgtactt tagtttccgg cgtgtaataa taatccaacc cggtttcacc cagcgcaaca
    39481 acgccctctt ctgccgccag acggcgtaaa tcttctacat cgtagggatc attctggttg
    39541 agtggatgca cgccacaaga aaatacgacg ttgtcacgtt cgcctaccag atcccgcatg
    39601 tgtaaataac ccggtaatgt tgtggcgact gccagacaaa atttcacatc gcgtgcggcg
    39661 gctttcgcca gaacgtcatc cacgtcctta tgcaaagatt cataatccag accatcgaga
    39721 tggcagtgtg agtcgactaa aaacatgatg tctctcttaa agatgaggaa ccggtagcac
    39781 aacgcccggt tgcaggtaat gctcaatacg cagtaaaaga tcggtgatga gaagctcgcg
    39841 gttaatacct gtgacagaca ttaactgttc acgaatgtgg caaacatccc ccagtatagc
    39901 ctgcaggcgc gagggagaaa gatggtttgc cagttcgacg accaggcccg gcacatcaac
    39961 attggtcacc tgcgcagcgc catgatggcg ttttagcgca tccatcagca acgttgccag
    40021 ccagtgtaaa cgcgccggag cttgttcatg attaagggcc gctagcagcg aataccagtc
    40081 gcccgatggc acgctatatg ccaaagcctg acacaatgtt tcacgagcct gccagttatc
    40141 tccgtgaaac aacgccagtg ccgcgccagg cgaaccggca cttaagcgca atgcggcaag
    40201 taatgcatcc tgtgacattg tcacttcgcg tgaaagccag gtcacggcgt actgttccgg
    40261 cggcggcgca aggtaatgta accgacaacg actacgtaat gttgccagta aacgttctgg
    40321 ctcgcgggta gcaaggaaaa accacgtttc tgctggtggc tcttcaagcg ttttcagcaa
    40381 tgcgttagcc gcggcgtcgg tcagtaaggc agcatcggtt acccagacaa ctttcgcacc
    40441 acctaagcgt gcgtgctcat tcagcttttc ggtgacctca cgtaccgcat caatgcccag
    40501 cgtatttttc cctttttcgg gagccagggt gtagtaatcg ggatgcgtgc cagcctgcat
    40561 caactgacat ccacgacagt gaccgcaact tttgtggccc tgcggttgtt ggcagagtaa
    40621 ataacggctc agggcgtaga ttaaagcatc atcgcccatg cccggtaacg cctgaatgag
    40681 cagcgcatgg tgacctcttc cggcctgata gctggctacc agtttttcga aatcaggtcg
    40741 taaccatgga taccatctca tgcgtccaac tccttcaccc agttggtcac ggtagtgcgg
    40801 attgcatcca tcacagcctc cagcggcagg gtggcatcaa tggtatgaat gcttttatct
    40861 tgtgctgcca gttccagata gcgggcgcgg gtgcgattaa aaaaatcgaa agactcttgc
    40921 tcaatacgat ccagctcgcc gcgtgcacgc gcgcgtttta agccaacttc cggggtaaca
    40981 tcgagataga gcgttaagtc ggggcgaaaa tccccgagaa cagcatcacg cagtgttgcc
    41041 agcatatgtt ggtcaatacc acgtccgccg ccctgatacg cctgagtgga gagatcgtgg
    41101 cgatcgccaa tcacccaagt gccgttagcc agcgcaggtt tgatgaccgt ttctaccagt
    41161 tgaacgcgcg cggcataaaa catcagaact tcggctttat cggtaatgac ttcatcgcct
    41221 accgatttga tatccagcac caggcttctt aacttttcgg caagttgcgt accgccaggt
    41281 tcccgagtga aaaccatgtc gcggataccc agttgctcaa gcgtctcaac caccacgtta
    41341 cgcgcggtag ttttgcctgc gccttccagc ccctcaatga cgatatactt actgcgcatt
    41401 tttttcctta agcactttca gataatcctg cacagacttg ttatgactgg caagattggt
    41461 attaaacgtg tgaccacctt taccatcggc cacaaaatag agatacggcg tttttgccgg
    41521 atgcgcagca gccttcagcg aatccgcccc cggcgtcgct atcgcacctg gcggcaaacc
    41581 ggtaatggta taggtgttat acgctgtcgg cgtttccagg tctgcacgag aaagtttgcc
    41641 attataacgc tctcccatcc cgtaaatcac ggtcgggtcg gtctgcaggc gcataccaat
    41701 gcgtaaccgg ttgataaata ccgaggcaac ctgatcgcgt tcactggcaa cggcggtttc
    41761 tttttcgata attgatgcca tcgtcaccaa ctggttttta tctttataag gcagaccgtc
    41821 cgcacgccct tcccaggcgc tatcgaccgc tttcaccatt ttcttgtgcg ctcgcttgag
    41881 taacgcgaca tcggtggtat tggcggtata catccaggtg tctggccaga accaaccttc
    41941 aatccactcc gggttttcca gttcaagtgc ctgcgctacg gtggcgtact tatcgtcgct
    42001 cagcgtgtgc ttgatatagg gggcctcacg caattgcttg aggtaatcgc tcagacgcat
    42061 cccttctacc agtcgcagag ggaactgtgc ttctttaccg ctttccagca atttcagcat
    42121 ctcgcgcacg gtcatctgcg gtgtaaagcg gtaagtcccg gctttaaagt gagaaagatc
    42181 cggttcgata cgcagcagcc attggaaaac ccgtgggcga ttgatgatct tatcggcata
    42241 aagctgttca ccgagcgcca gacgtccggt ccctggcttc agggtaaata tcgtctcttc
    42301 tttgataagt aatttgctgt cggcaagatg gcgaagcttc cagacgccca caccagcggc
    42361 gatacccagt accaccagca ataacaagat aatcaataac acttttttca tgactaattc
    42421 gggcgctcac aaagtggggc taaatattca tataacgttg ctgacgaaaa ggagacatcg
    42481 ccacaggcac gtacgggcat cactggcatt aacgcattac aaataaccat ctcatctgcc
    42541 tgcaacgcct cctccagcga ggcttgcact tcgacaagct gataagagga ttgtgccagc
    42601 aaacggatac agaattgtcg cataatgccg ttaacacctg cctgatccag tcgcggcgta
    42661 taaactacgt tgcccttccg ccagaacaaa ttagccgcac agcattccgt aacccaccct
    42721 tcgctgtcaa ggaccagcgc ctcatcagcg tttgtctgct caagatgaga gcggatcaat
    42781 acttgctcaa gccgattaag atgtttaata cctgcaagat gaggattgcg ccccagccgc
    42841 accgggctta gcgccaacgt catcccctcg ttacgcaaac ggtcgtaatg tgcgggataa
    42901 accgtaacgg agagaatccg cgttgccggt ccgctgttca atgtgctgta tcctcgcccg
    42961 ccactaccgc gactgatcac cactttcagc acaccatttt gctgttctgc tgccagcgtt
    43021 ttcatctcta gttcaagctg aggccagaag tcacaggaaa tcatcaaccg ctgacaagca
    43081 tcctgtagtc gctggatatg cgccgataac aaactgactt taccgtcgat aactctggcg
    43141 gtggtaaaac aaccatcacc aaactgcgtt gcccgatcgc ttactgccag cgattcctgc
    43201 ttatgaccgt taattaagaa cataccggct ccttatcaga gaattgttag tgtggcagca
    43261 tgttcactac ggaacaagtc ggaataaaag ctaagaaaaa aggcccgcaa gcggaccttt
    43321 tataagggtg ggaaatgaca acttagatct ttttaaagat caaagaacca ttagtgccac
    43381 cgaagccgaa ggagttacac agcgtgtatt ccattccgct gacctgacgt gcttcgtgcg
    43441 gtacgaaatc cagatcgcaa ccttcatccg ggttatccag gttgatggtt ggcggaacag
    43501 cctgatcgcg cagcgccagg atggagtaga tagattctac tgcacccgcc gcacctaaca
    43561 ggtgaccggt catagatttc gtggagctta ccaacacacg gcttgcagct tcaccgaaga
    43621 tggttttcac cgcctgcgct tcagctttat cgccagccgg cgtagaagta ccgtgagcgt
    43681 taacgtagcc aatctgactc gcttcaatgc ctgcatcacg cagagcattt gccattgcca
    43741 gagctgcgcc tgcgccattt tctggcggtg acgtcatatg ataagcatcg ctgctcatac
    43801 caaagccgac gagttcagcg taaattttcg caccgcgttt tttcgcgtgt tcgtactctt
    43861 caagtaccag cataccggca ccatcgccca gtacaaaacc atcacgctct ttatcccacg
    43921 ggcggctcgc cgcttgcggg ttatcattgc gggtagataa tgcacgtgcc gcgccaaaac
    43981 caccaacgcc cagcggcgta ctggctttct ctgcgccacc tgcaaccatc acgtcagcat
    44041 cgccatacgc gataatacgc gcagcatggc caatgttgtg cacgccggaa gtacaggcgg
    44101 tcgcgataga gatgcttggg ccacgcaggc catacatgat agtcagatga cctgccacca
    44161 tgttcacaat cgttgacgga acgaagaatg ggctgatctt acgtggacca ccgttcatca
    44221 gagatgtgtg gttttcttcg atcagtccga ggccgccaat cccggagcca attgcggcac
    44281 caatgcgggt tgcgttctct tccgttattt caaggccaga atcctgcatg gcctgaacgc
    44341 cagcgacaat tccatattga atgaaggcat ccatcttgcg ctgttctttg cgcgagataa
    44401 tgtcctcaca gttaaaatcc tttactaagc cagcaaattt cgttgcatag gcgctagtat
    44461 cgaaatggtc gattaggctg atgccactct gaccggcaag cagagctttc caggtagact
    44521 ctacggtatt gccgacagga gacaacatgc ccagtccggt cacaactaca cgacgcttag
    44581 acacgtttgt cctccaggga gggaaaaaat gattctagtg ggacaaaaag ataaaactca
    44641 ggcggtcgaa cgaccgcctg gagatgttca cttacgcctg gtggccgttg atgtaatcaa
    44701 tggcagcctg aacggtggtg attttctcag cttcttcgtc cggaatctca gtatcaaact
    44761 cttcttccag agccattacc agctcaacgg tgtcaagaga atccgcgccc aggtcttcaa
    44821 cgaaagaagc attgttggta acttcttcct gcttaacgcc cagctgttcg ccgataattt
    44881 tcttaacgcg ttcttcgata gtgctcatac tcttaaattt cctatcaaaa ctcgctttcg
    44941 cgatggtttt cgtagtgtat aaaatgttga aaaatttgca actaaatccc ggcaggtctt
    45001 accacgattt tacgttattt tgaggcctta ccccccaata acgcaaatca ttttgcacta
    45061 attattactc ataaccacgc agactgcgcg caacatgagc attttgtgca aatcgcggtc
    45121 agaccatgta catcccgccg ttcacatgca aagtttcacc cgtgatgtaa gctgcttcgt
    45181 cggatgccag gaatgcaacc gcgttggcga tttcctgtgc gccgccgagg cgacccgcag
    45241 gaacctgcgc caggataccc gcacgctggt catcgctcag cgcacgtgtc atgtccgttt
    45301 caataaagcc cggagcaaca acgtttacag taataccgcg tgacgcaact tcgcgcgcca
    45361 gtgatttact gaagccgatc aagcccgctt tcgccgcagc gtagttggcc tgaccgccat
    45421 ttcccatggt accaaccaca gaaccgatag tgataatacg accatgacgc tttttcatca
    45481 tagcgcgcat taccgctttt gacagacgga aaacagatga aaggttggtt tcgataatat
    45541 cgttccactc ttcatctttc attcgcatta acaggttatc acgagtgata ccggcattat
    45601 tgaccaggat atccacttca ccaaattctg cgcgaatttt ttccagaaca gattcgatag
    45661 atgccgggtc ggtcacattc aacatcagac ctttgccgtt ggcacctaaa taatcagtga
    45721 tcgcctgagc gccattttca ctggtcgcag tgccgataac tttcgcgcca cgggctgcga
    45781 gcgtttcagc aattgcgcgg ccaattccgc ggcttgcacc ggttaccagt gcgatttttc
    45841 cttcaaaatt catgattttc ctcttttaaa gctcgagcgc cgctgccatc gctgaaggtt
    45901 cgttcagcgc cgaggcggtc agggtgtcga caatgcgttt cgtcaggcca gtaagcactt
    45961 tgcccgggcc gacttcatag agatgttcta cgccttgcgc tgccatgtat tcaacagact
    46021 tcgtccactg aaccgggtta tacaactgac gtaccagtgc gtcacggatg gcatcaccat
    46081 tggtttcgca tttcacatca acgttattca caacaggaac tgttggtgcg ttaaaggtga
    46141 ttttcgctaa ttctactgcc agtttgtcgg ctgctggttt catcagcgca cagtgagacg
    46201 gtacgctcac tggtaacggc agagcgcgtt ttgcgcccgc agctttacag gcagcgccag
    46261 cacgctcaac tgcttcttta tgaccggcaa taaccacctg tcccggagag ttaaagttta
    46321 ccggagaaac gacctgacct tctgcagctt cttcacacgc tttcgcaata gacgcatcat
    46381 ccagaccgat gattgccgcc atagcacccg ttccttccgg tacggcttct tgcatgaact
    46441 tgccgcgcat ctcaaccaga cgcaccgcat cagcgaaatc aatcacacca gcgcaaacca
    46501 gcgcggagta ttcccccagg ctgtgaccag ccatcattgc cggtgcttta ccgccctgct
    46561 gctgccatac gcgatacagc gcaacagatg cagtcaacag cgctggctgg gtttgccagg
    46621 ttttattcag ttcttcagcc ggcccctgct gggtcagcgc ccacaggtcg tagcccagcg
    46681 ccgcagaagc ttcagcaaac gtttcttcga caattggata gctcgccgcc atatcagcca
    46741 gcattccaac ggtttgagaa ccctgtccag ggaacacaaa tgcaaattgc gtcatgtttt
    46801 aatccttatc ctagaaacga accagcgcgg agccccaggt gaatccaccg ccaaaggctt
    46861 caagcagaac caactgcccc ggcttaatgc gcccgtcgcg tacagcttca tccagagcgc
    46921 acgggacaga ggccgcagag gtattaccgt ggcgatccag cgtcaccacg acattgtcca
    46981 tcgacatgcc gagttttttc gctgttgcac tgataatacg caggttagcc tgatgcggaa
    47041 ccagccagtc cagttgagaa cggtcaagat tattcgccgc cagcgtctca tctacgatgt
    47101 gcgccagttc tgttaccgca accttgaaga cttcgttgcc cgccatcgtc agatgaattg
    47161 aattctctgg attcacacgg tcagcattag gcagcgtcag caactcacca tagctaccgt
    47221 cggcatgcag atgggtggag atgattcccg gctcttcaga ggcagccagc accgcagcgc
    47281 ccgcgccatc gccaaaaata ataatagtcc cacgatcggt tggatcgcag gtgcgcgcca
    47341 gtacatcgga accgacgacc agagcatact tcaccgcccc agatttcacg tattgatcgg
    47401 ctacgcttaa tgcataggtg aaacctgcgc aggctgctgc aacgtcaaat gccgggcaac
    47461 ctttaatgcc cagcatgctt tgaatctgac aagctgcgct cgggaaagcg tgcgtagcag
    47521 aagtcgttgc cacaacgatc aggccaatct ggtctttctc aatgcccgcc atctcaattg
    47581 cgcgtgtcgc cgcttcaaag cccatggttg aaacggtttc gtttggcgcg gcaatgtggc
    47641 gttcgcggat accggtacga gtgacaatcc actcgtcaga ggtgtccacc attttttcca
    47701 aatcggcgtt tgtccgcact tgttcgggca gatagctgcc agtaccaata atcttcgtat
    47761 acatgtacgc tcagtcactt ttcggttata taccgtcact tgcaaactgc gagttcgctg
    47821 gcagcgtcct gctaccgcag agttccgctt ttgccaccgt ccagcagctc aaaaccagct
    47881 gggtatacag attccaggcg agcggcaatt cgctgaggaa cttgtcgctg caccgcctgc
    47941 actgcctgtt caatcgcgac cgcaaaagct cgctgattgg ctgcaccatg actttttatc
    48001 accgtgccgc gcaatcctaa cagacaggcg ccgttatact ggtcggggtt gaggtgactg
    48061 aatcgcctcg tcaggctctt ttgtagccaa cgctttaata acagtagcca ccacgaccgt
    48121 tttttccctt caccctgaga tttcagcaga gaaaggaaca tcctgacaac accttccatc
    48181 gtctttaatg tgacatttcc tgtaaagccg tcacaaacca gcacatctgt cttgccagtt
    48241 aacaactcat tggcttcaag atagccgata taattgatag aagggattgt tttaagcacc
    48301 gctgaggcat cccgaatact gtcgagaccc tttacttctt cttcaccaat attgagcaac
    48361 gccacgcgag gattaggaat ttccaccacc tcttcagcca gaactgagcc cataatggca
    48421 aattgcacca acattgtact gtcacaatcg acgttggccc ctaaatcgag gaccaccgtt
    48481 ttgccctttt gctgatgcgg taataccgtc accagcgccg gacgctcaat cccctccagg
    48541 ggcttgagta ataattttgc cagccccatt agcgccccgg tattaccggc actgacacag
    48601 gcttgcgctc gaccttcttt caccagctcc agtgccacgc gcattgaact cccacgactg
    48661 gcgcggatag cttgcgaagg ccgggcatca ctggcgataa ctgactgcgc aggaataatc
    48721 tgcagacgcg aacgttgttc aaagtcagct ttagcaagta atggcgtgat ggcgtcggga
    48781 ttgccgacta aaagaagagt gagttgcgaa ttagaattca gtgcctgcaa tgctgcaggc
    48841 actgtcacgg aagggccaaa atcccctccc atgacatcta acgccagggt tagacgtgtc
    48901 aaggtatcgt cgccgcccgg tttggtcttt ccccagttgc ctggggaaaa tcctcactaa
    48961 gcttcatcac gcagacgcgt gattacttag cgatgacctt gcggccgcgg tagtaaccgt
    49021 cggcagtgat gtggtgacgc aggtgttttt caccagaagt tttgtctaca gacaggctgg
    49081 tgactgcggt cagcgcgtca tgggaacgac gcatgccacg tttggaacgg gttggtttat
    49141 tctgttgtac ggccatggac cttactcctc aattacggtt tatccccgat ccaacgggga
    49201 gcaccaatta cttacgcttt aagctggcta atacggcaaa tgggtttggc ttttgcgctt
    49261 cttcaggcag ttcaccaaag accatgtccg cttcggacac ttcacagtgt tcagaatcat
    49321 gcaccggaac taccggcaag gcgaggatga tttcatcttc aaccattgca agcagatcga
    49381 tttcaccgaa ttcgttaacc tcaatcggtt catacgcttc cggcagtgct tcagcctgtt
    49441 cgtctgaacg cacaggacta aaacaatacg ttgtgtagac ctgatgagta aacggcttcc
    49501 cgcaacgctg acactcgagc gttaccgtca ccttcgcatc gccgtttaac actgcgagac
    49561 gttggttatc gatagcgaac gacatggagc attccacatc actgtccaca ctgactacgg
    49621 attcggcgac gcgctcaacc tgatcaggag tatagatacc ctggtaatca aggcgttttt
    49681 gagccgtacg aaccggatcg agagtcaggg gtaattttac cttttgcata gggcgcgcat
    49741 attaactttg taacgtcata gagtcaaaga aaaaggcagc ctgtggttgc cttttgccaa
    49801 taattcgcac acattgcggg ttactgcttt attttcgttc agtgctgata acgaacatca
    49861 acctgcaaaa cgaagcgcat agtttaaaat ggctatcatc aatacgctat atctgatgga
    49921 aaaaaatatg cctaaactta ttttagcctc cacctcgccc tggcgtcgcg ctcttctgga
    49981 aaaactgcaa atctcttttg aatgtgcagc acccgaggtc gacgaaactc cacgcagcga
    50041 cgaatcaccg cgacagttgg tgcttcgact ggcacaagaa aaagcgcaat ctctggcgtc
    50101 acgttatccg gatcatttaa ttattggttc agaccaggta tgtgttcttg atggcgaaat
    50161 caccggtaaa ccgttaacgg aagaaaatgc ccgtctgcaa ttacgcaaag ccagcggcaa
    50221 tatcgtcacc ttctatactg gactggcgtt gtttaattcg gcgaatgggc atctacaaac
    50281 agaagtggag ccttttgacg tccatttccg tcacctgagc gaggcggaga ttgataatta
    50341 cgtgcgtaaa gagcatcccc tgcactgcgc gggtagcttt aagagtgaag gatttggcat
    50401 tacgctgttt gagcgtttag aggggcgaga tcctaacacg ctggttggtt taccgctaat
    50461 cgcgctgtgt cagatgttac gtcgggaagg gaaaaacccg ctgatgggat agttttctgc
    50521 gccggatgcg gttacgtcgc atccggcaat cggtgctggc ggcctgatgc ggcgtaaacg
    50581 ccttatcaag gccaaccgcc cattatcagg tttatatgct tgtattagcg cgcgttacgc
    50641 agcttttgca aacaacgctt caaaccttca tccatcggcg cttcgatacg catcacctca
    50701 ccggtccccg gatgggtaaa cttcaacgct gcggcgtgca ggaacagacg atttaatcct
    50761 gtgcctgctt cagtgagctg tctgtcaaat tcacggtcac cgtagcgatc gtcaaaggca
    50821 atcggatgac ccgcatactg tgtatgcaca cggatctgat gagtacgccc tgttaccggg
    50881 ctacaacgca ccagggtggc aaaagcatag cgttcttcca ctttaaagcg tgtttccgac
    50941 ggtttgcctt cctgactcac acgcacgata cgttcgccgc tttgcagaat atttttcagc
    51001 aacggcgctt gaacgctctt cacatgcgac tgccactgac cgcgcaccag cgccaggtaa
    51061 tctttttgca tccctttttc acgtaattgc tcatgcagag aacgcaacgc cgagcgtttt
    51121 ttcgctacca gcaacacacc tgaagtgtcc cggtcgagac gatgaaccag ttcaaggaac
    51181 cgcgcttccg ggcgcaacgc ccgcaaacct tcgataacgc cgaagcttaa accactgccg
    51241 ccatgtaccg ccgtaccgga aggtttattc agcaccagga tatggtcatc ttcatataag
    51301 atgacgtccg ccagcgccgc caccttttgc agatgtggcg aaaccgcctc ttcttcccgt
    51361 tcagcaacgc gaaccggtgg gatacgcacc tcatcacccg cttcgagttt atattcaggc
    51421 ttaatacgtt ttttgttcac ccgcacttcg cctttacgca aaatacggta aatcatactt
    51481 tttggtacgc ctttcaattg ggtacgcaaa aagttatcga tacgttgccc cgcttcgtca
    51541 gcgttgatag caacaatttt tacggatgga gtctctgttt tcatggtggg cgattctaat
    51601 tagccaacag gattcgcgcc actcattttt ctatgcttat atttagtttt gcaccttatt
    51661 acttcactac gtgatcactt tattgatggt tattaaacca atcaccagca agaagtgaaa
    51721 aaactgtgag taagcgggtg ataaatggta aaagtcatct tgctataaca aggcttgcag
    51781 tggaataatg aggccgtttc cgtgtccatc cttgttaaaa caagaaattt tacggaataa
    51841 cccattttgc ccgaccgatc atccacgcag caatggcgta agacgtattg atctttcagg
    51901 cagttagcgg gctgcgggtt gcagtcctta ccggtagatg gaaatatttc tggagagtaa
    51961 tacccagtct gtttctttga taattgcgct gtttttccgc atgaaaaacg ggcaaccgac
    52021 actctgcgcc tctttgagct gacgataacc gtgaggttgg cgacgcgact agacacgagg
    52081 ccatcggttc acacccggaa aggcgttact ttgcccgcag cttagtcgtc aatgtaagaa
    52141 taatgagtaa gttacgatga aaagaatgtt aatcaacgca actcagcagg aagagttgcg
    52201 cgttgccctt gtagatgggc agcgtctgta tgacctggat atcgaaagtc cagggcacga
    52261 gcagaaaaag gcaaacatct acaaaggtaa aatcacccgc attgaaccga gtctggaagc
    52321 tgcttttgtt gattacggcg ctgaacgtca cggtttcctc ccactaaaag aaattgcccg
    52381 cgaatatttc cctgctaact acagtgctca tggtcgtccc aacattaaag atgtgttgcg
    52441 tgaaggtcag gaagtcattg ttcagatcga taaagaagag cgcggcaaca aaggcgcggc
    52501 attaaccacc tttatcagtc tggcgggtag ctatctggtt ctgatgccga acaacccgcg
    52561 cgcgggtggc atttctcgtc gtatcgaagg cgacgatcgt accgaattaa aagaagcact
    52621 ggcaagcctt gaactgccgg aaggcatggg acttatcgtg cgcaccgctg gcgtcggcaa
    52681 atctgctgag gcgctgcaat gggatttaag cttccgtctg aaacactggg aagccatcaa
    52741 aaaagccgct gaaagccgcc ctgctccatt cctgattcat caggagagca acgtcatcgt
    52801 tcgcgcattc cgcgattact tacgtcagga tatcggcgaa atccttatcg ataacccgaa
    52861 agtgctcgaa ctggcacgcc agcatatcgc tgcattaggt cgcccggatt tcagcagcaa
    52921 aatcaaactg tacaccggcg agatcccgct gttcagccac taccagattg agtcacagat
    52981 tgagtccgcc ttccagcgtg aagttcgtct gccatctggt ggttccattg ttatcgacag
    53041 caccgaagcg ttaaccgcca tcgacatcaa ctccgcacgc gcgacccgcg gcggcgatat
    53101 cgaagaaacc gcgtttaaca ccaacctcga agctgccgat gagattgctc gtcagctgcg
    53161 cctgcgtgac ctcggcggcc tgattgttat cgacttcatc gacatgacgc cagtacgcca
    53221 ccagcgtgcg gtagaaaacc gtctgcgtga agcggtgcgt caggaccggg cgcgtattca
    53281 aatcagccat atttctcgct ttggcctgct ggaaatgtcc cgtcagcgtc tgagcccatc
    53341 actgggtgaa tccagccatc acgtctgccc gcgctgctcc ggtaccggta ccgtgcgtga
    53401 caacgaatcg ttgtcgctct ctattctgcg tctgatcgaa gaagaagcgc tgaaagagaa
    53461 cactcaggaa gttcacgcca ttgttcctgt gccaatcgcg tcttacctgc tgaatgaaaa
    53521 acgttctgca gtgaatgcga ttgaaactcg tcaggacggc gtgcgctgtg tgattgtgcc
    53581 aaacgatcag atggaaaccc cgcactacca tgtgctgcgc gtgcgtaaag gggaagaaac
    53641 tccaacctta agctacatgc tgccgaagct gcatgaagaa gcgatggcgc tgccgtctga
    53701 agaagagttc gctgaacgta agcgtcctga acaacctgcg ctggcaacct ttgccatgcc
    53761 ggatgtgccg ccagctccaa ccccagctga acctgccgcg cctgttgtag ccccagcacc
    53821 taaagctgca acggcaacac cagcatctcc tgcacaacca gggttgttga gccgcttctt
    53881 cggcgcactg aaagcgctgt tcagcggtgg tgaagaaacc aaaccgaccg agcaaccagc
    53941 accgaaagca gaagcgaaac cggaacgtca acaggatcgt cgcaagcctc gtcagaacaa
    54001 ccgccgtgac cgtaatgagc gccgcgacac ccgtagtgaa cgtgctgaag gcaacgataa
    54061 tcgcgaagaa aaccgtcgta atcgtcgcca ggcacagcag cagactgccg agacgcgtga
    54121 gagccgtcag caggctgagg taacggaaaa agcacgtacc accgacgagc agcaagcgcc
    54181 gcgtcgtgaa cgtagccgcc gccgtaacga tgataaacgt caggcgcaac aagaagcgaa
    54241 ggcgctgaat gttgaagagc aatctgttca ggaaaccgaa caggaggaac gtgtacgtcc
    54301 ggttcagccg cgtcgtaaac agcgtcagct caatcagaaa gtgcgttacg agcaaagcgt
    54361 agccgaagaa gcggtagtcg caccggtggt tgaagaaact gtcgctgccg aaccaattgt
    54421 tcaggaagcg ccagctccac gcacagaact ggtgaaagtc ccgctgccag tcgtagcgca
    54481 aactgcacca gaacagcaag aagagaacaa tgctgataac cgtgacaacg gtggcatgcc
    54541 gcgtcgttct cgccgctcgc ctcgtcacct gcgcgtgagt ggtcagcgtc gtcgtcgcta
    54601 tcgtgacgag cgttatccaa cccaatcgcc aatgccgttg accgtagcgt gcgcgtctcc
    54661 ggaactggcc tctggcaaag tctggatccg ctatccaatt gtacgtccgc aagatgtaca
    54721 ggtcgaagag cagcgcgaac aggaagaagt acaagtgcag ccgatggtga ctgaggtccc
    54781 tgtcgccgcc gctgtcgaac cggttgttag cgcgccagtt gttgaagaag tggctgaagt
    54841 tgtagaagcc cccgttcagg ttgccgaacc gcaaccggaa gtggctgaaa cgacgcatcc
    54901 tgaagtaatt gccgccgcgg taactgaaca gccgcaggtg attaccgagt ctgatgttgc
    54961 cgtagcccag gaagttgcag aacacgcaga accggtggtt gaaccgcagg aagagacggc
    55021 tgatattgaa gaagttgccg aaactgctga ggttgtggtt gctgaacctg aagttgttgc
    55081 tcaacctgcc gcgccagtcg tcgctgaagt cgcagcagaa gttgaaacgg tagccgcggt
    55141 tgaaccagag atcaccgttg agcataacca cgctaccgcg ccaatgacgc gcgctccggc
    55201 accggaatat gttccggagg caccgcgtca cagtgactgg cagcgcccta cttttgcctt
    55261 cgaaggcaaa ggtgccgcag gtggtcatac ggcaacgcat catgcctctg ccgctcctgc
    55321 gcgtccgcaa cctgttgagt aataattagc tcaagtaatc aagccctggt aactgccagg
    55381 gcttttttat ttcaggggta gcatctttgc tgtaatgaca ttagcgttac atgtactgta
    55441 taattatcat ccacgcaaag ccgaaggata tatttatgcc agcagccaat aacattccag
    55501 ccaggcgcga atctttaaac ttgcgcataa aacccgcaga acgcgatctt attgaccggg
    55561 ccgctaaagc ccgaggaaaa aatcgcaccg aatttgtcct tgaggccgcc agagccgcag
    55621 ctgaagaggc gctcattgag cagagaattt tcaggatcga ttctgatgat tatcagctat
    55681 ttttgaatcg tctggatcag ccccccgcgc ccaatgctgc attacgcaaa accatgcaaa
    55741 cacccgcgcc gtgggagcaa aaaaaatgat tcaggccccc gagccactca tggcaaggca
    55801 tcaattcacc tctttttgct cgggagtaga aacgatggat aactggctaa agcagagagc
    55861 cctaaaaaat cagcttgccg gcgcatcacg tacatttgtt agctgtgata cgtatagcaa
    55921 cgttttggca tattattcgc ttgcttccag cgccgttgaa acgtatgtcg cgacaggacg
    55981 atttcgccga aatatgccag aaccgatccc tgttgtagta cttggtcgtc tggcaataga
    56041 taaatcatta cagggtcagg gcatcgatcg tgcgatggtc agagatgccg gtctacgagt
    56101 actacaggcg gctgaagtca ttggtattcg cggaatgttg gtccatgcgt tatcagacca
    56161 ggcccgtgag ttttatctgc gagtggggtt tgaaccatcg cctgtcgatt ctatgatact
    56221 gatggcaaca ttggctgatt tgcaggaatg tcttaaatag ttcgttatgt ctcatgcaaa
    56281 aaaatgcccg ccccggcgat cccagagcag gcagacaaaa catacccagt ttcatgatat
    56341 gttttaaagc gaaattattt gttgagctgg aagagcgaca atccctgcat atcggtaaat
    56401 gctttatacg acgcctgcaa tgccgtttgc tgcatgatgt aagatgaaat agttgcattc
    56461 cagtccacat caaccagatc gctcatctgc tgcgtttgcc ctaaagcgcg atcgctacct
    56521 aatgaatcca gcgactccag ttcgttcagc tgcgtgccta attccgcgcg aacagtcagc
    56581 acattgttca gcgagttttt cagtccgcgg ttggttttat ccagtgccgc agcggcggtt
    56641 tctttatccg cttcgctatc cgcgaccggc gttttcaggg ctgcgatggc gctatccagc
    56701 atggcaaaaa gattggtttc agaagcgcta ccgtctggtt ccgctaccgc gttgctggta
    56761 atactgtcga aaattttgtc acccgtgtgc cctatcacca tcgaacgcga agcatcgacc
    56821 tgttgtttaa tactttctgc tccgccttcg tattttccat ccgcctcgct aaacggtgcg
    56881 gattctgttt tataaccggc aaaaatgtag cgcccgttac cgtcagtggt gttcgccaga
    56941 ttcagcaact ggtcacgaag cccctgaata tccgtagcca gcgaggcccg gtcatcgtca
    57001 ctcaaggtgc cattgctggc gtagacaatt ttttcctgag cattctggat agcggtggtg
    57061 acctggctaa gtacactctc ttccagtgac actttttgag tggcgaaagt acgcgccagc
    57121 gtgtactggc tgttttgcgc ctgtgcctgg gagagaacaa cagcttgtga tgcagcaatg
    57181 ggatcatctg aagggttaac gactcgctta cccgtcgaca tctgttcgcc gtacttcatc
    57241 cattctgcct gagaattggt gataccacgc atgttttgct ggtacatcat ctgggtactg
    57301 aaacgcatct tatctcccct tagcgaatgt taatcagcgc atcaaaaatc gcgtttgccg
    57361 tctgcagaac ctgcgcattc gccaggtaat actgctgaaa acgttgcaga tttccgtact
    57421 cctcatcgag attgacaccg gaaatcgact gctgctgatt ggaaagctgc gtcaccacat
    57481 taccttgcgt ggtgctactg gttttcaacg tcgcggtttt attaccgata tcactcacta
    57541 acgaggcata agcgtcgtta aaggatttcg caccgcccac cgttttactg ttgctttgca
    57601 gatcgagcag ggcctgaccg ttgcggttgt cgctatcacc tgtatcttct gcgctcgcca
    57661 tggcgatttt cgcttcgtcg gtgattaata catccatgtt gacgatggcg tcacttaccg
    57721 gtttcagcgt gaagctgtcg ttaacggcag gcgttcctgt aaacgtcaac tccagaccat
    57781 caaatgccac tttaccgctg gcatccggcg tcaccgtaaa agtggtatta ctggcaaggc
    57841 gggtgacctg ccactgatta ttatcgaacg agattttgta atctgtcgcc agtaccgcgg
    57901 aggcatcagt taccgttgca ccgatcgcaa cgtcaccttt gtttttcgtg ttttgcagaa
    57961 ccgcgggctt accgatagca aagaaatctt caccggcatc gccgttagca tcaaatccgg
    58021 ctttgtgttg ggtgttgaaa gcctcggcaa atgccagcgc cagttgtcca agcgtattac
    58081 gcgtctggtc cagatcctga gaacggaatg tcagaatgcc gcccagcgac ccggtattca
    58141 gtaatttctc cgggatctca atattgcctg ccgtcccatc aacataagcg acagtcgtac
    58201 gagaagggtc agcgctggaa ggaactgccg ccagttgccg agccgtactt ccctgaacca
    58261 gtgagtaacc attggccatc gtgatgttat aagtgccacc atcctgaacg ctgacttcta
    58321 caccaacaat ctggtttaat tcgctcacca gttgatcgcg ttgatccagc agattgttag
    58381 gtgacgcccc tgcccccacg cctgtcaggc gggagatttg atcgttcagg ctggcgattt
    58441 gtttagcgta attgttgatc tgatcaacgc tggcacctat cgcgatattg acctgtttgt
    58501 cctggtcgcg cagatattga tcggtggttt taaactgatt caccaaccct tctgatttcc
    58561 caatcagcgc ctggcgcgct gccggatctt ccgcgttact caccagcgtt tgcaggctgg
    58621 tgaagaaatc ctgcatctgt gttgccagcg aagaggtact ggtggagagc atattgtcga
    58681 ttttcgacat ctgctcatag cgggcggtca ggccgctact ttgcgtctgc gcagcacgta
    58741 actggttggt aataaacgca tcatactcac gctgcacacc agaaacgtag acgccattgc
    58801 caacccagcc gccagcgccc aacgtgctat tggcctgcgc cataatagtg gtttggcggg
    58861 tatatccggc aacgttatag ctggagatat tattacttgc cgtatttaac gccgcctggg
    58921 ccgcgttcag tccgctcatg gcgttattga tcaagctgga catggaggtt ccttataagc
    58981 cttcaattac agagtattat cggcagcgcc ccgccggact tgagttattc agaacagatt
    59041 atcaatgttc atgctgtagg ttttgctcac cttgtcgctt atcgatttca tctgctgaat
    59101 catgctggtg agtttgcggg catagtgagg atcggtggca tagcccgcgt cctgtagggc
    59161 ctgcgccccc tgctccgcac tcgcggcggt cgtcacggcg gcgtagcgcg ggttacgcgt
    59221 taacagccca acgtaatccg acaatgcttc cagatacgag ctgtagaccc gaaacttcgc
    59281 ttttactttc ttcgcttcgc cattttcata ttcagtcgtg gtgatttcag tgactggccc
    59341 tttccagttg ccagaggctt tgacgccaaa caggttatag ctcggctcgc cgttttcacg
    59401 gcggatttgc cgttgtcccc agccagattc cagcgccgcc tgagcgagga tcaaatgatg
    59461 tggcacaccg ctttgctggc ttgccagttg ggcgggcaac gagagttgcg cgaggaatgc
    59521 tttactgtca cccggcagcg aatcatcgta gttacgtggt acggcctttt gcaccagctg
    59581 cgaaagcgtc tgattttgat aacgcaccac ggtttcgagc gggaatttca tcggtgctgc
    59641 tggcgtggac tcctctggca atggttgttc tggcgtcatc tgtttaacca tcatctctgc
    59701 cagccccaga cctttgcccg ccgtcatctg ttgggcaatc tgctggtcat acatactggt
    59761 atacaggcga gtgtgctcgc tgctgaacag gccatctttt ggtaaagcgt cgcgcatgct
    59821 tttcaacatc atctgcacga acatcccttc cacctggcgg gccaccggac ggatatttgc
    59881 cgccggatct tcgcccgctt tcgccttaag ttcgttgagc gattgtgcat cccaggccgc
    59941 acttgccagt agtttgctgt cgctgatcat cagatgattt ccagttttgc ccgcagacat
    60001 cccgcacttt gcattgattg caaaatagac atcagatcca tcggcgtagc gcccagcgca
    60061 ttgagcgcac gcaccacgtt attgaggctg gcgctggaac gtacgctttg cagcgaaccg
    60121 ccgctctggc gtaaatcgat ctgcgtttgt ggagtaacca cagtctgtcc accaccaaac
    60181 ggtgtatctg gctggctgac attggcctgg cgattaactg ttactgagag atttccctgc
    60241 gctaccgcgc agctgtcgag ggtcacttcg cgattcatca ccaccgaacc ggtgcgcgag
    60301 ttaatcacta ctttagcgtc ctgcggggta acattaacct gcatattctg gatatcggca
    60361 aggaaacgga cctgggaact gttgccactc ggtacgcgca cctgaatagt ccgcgcatcc
    60421 aacgcggtgg cgctgccata tccacgcact cggttgatgg tgtcagcgat ttgctgtgcc
    60481 atgctgaaat cttcgtcgtt aagttgcaaa ttaagggtat tcccgacgcc aaactggctg
    60541 ggcaattcac gttcaataac cgcaccattg gtgatccgtc caccgttcag ttggttcacc
    60601 tgaacactgc taccgccagc ggaggcccct gcgccgccaa ccagaatatt gccctgcgcc
    60661 agcgcataca cctgactgtc aacgccctta agcggtgtca tcaataacgt accgccacgc
    60721 agacttttgg catttcccat ggaagaaacc accacgtcaa tggtttgccc ctgacgtcca
    60781 aacggtggaa gtgacgctgt caccataacc gcagcgacgt tttttagctg catattagtg
    60841 cccgtcggaa cggtaattcc cagctgtgag agcatgttat taagcgtttg tgtggtaaac
    60901 ggcgtctgag ttgtctggtc accagttcca tccagcccca ccaccaggcc atagccaatc
    60961 agtgagtttt gccttacccc ctgaacactg gtgagatcgc gaatacgctc agcctgagcc
    61021 gccgtcgtaa ccagtagaag aattaatgca gagagaaatt taatcaccac agcctcactt
    61081 acattggaga caggttaaga aagaaacgct gcaaccagcc catattttgc gcttcgttaa
    61141 tgtagccatt gcctacgtat tcaatgcgcg catccgccac ctgagtagac ggtacggtat
    61201 tgctgccgct gatagtgcgt ggattaacca cgccagagaa gcgaataaat tcggtaccct
    61261 gattaatggc gatctgtttt tcacccacca catgcaggtt gccgttaacc agtacctggt
    61321 caaccgtcac cgtcaacgtg ccgctaaagg tattactggc attggccccg ccttttccgt
    61381 tgaacgtgtt accaccggag gcttcgacat cggcacgagc gttaccaaac agcccctgca
    61441 aatagcgcgg cacagtatca aagccaaaat tagttttacc gtcacggctg gcattcgcag
    61501 aggagctttt gctggcgctg acgttctcct gcaacacgat ggtcagcgta tcgccaatat
    61561 tgcgtggtcg acgatcttca aacagcggtt gatagccata gttaatcggc tgagcagact
    61621 ggaaaataga accgttggcg acgggcgtcg gaccgggaac cggttgtgca ctggtcgccc
    61681 cctgtaccag cggcgtggag ggtatccagg cgcagccggt tagtgaaagc accaacaagc
    61741 tggaaatggc ataagtatgc gcagcgtttt tttgcatggc tatcatcttc aaaaatcagt
    61801 aaaccggtga atctgccacc ggttatgcct tagagttgcg tcagtttttg cagcatctga
    61861 tcggtggtgg ataccgcttt actgttgatt tcgtaagcgc gttgcacctg aatcatattg
    61921 accagttctt ccgccacgtt gacgttagac gtttcaacat acccttgata cagcagtccc
    61981 gcgccgttca ggcccggcgt gctttcgttc ggtgcaccgg aggattgcgt ttcggtgtag
    62041 aggttttcgc caatactctc cagtccggta tcattcataa aggtggtgag attgagctgc
    62101 ccaacctgaa ccggagctgc ctggccttgt tgtgttacgc tgaccacgcc atcacgaccg
    62161 atggtgatac ttagcgcatt cgccggaatg gtgatcgctg gctgcacctg aaaaccacct
    62221 gccgtcacca gctgcccgtt ctgatccacc tggaaagagc cgtcacgggt ataggctgat
    62281 gagccatctg gcaacatcac ctggaaaaag ccctgccctt taatcgcgac atctttgctg
    62341 ttgttggtct gcgacagatt gccctggctg tgtaagcgtt cagtggcgac cgggcgtaca
    62401 cccgtgccga tttgtaatcc ggatggtaag gtggtttgtt cggaagactg agcacctggc
    62461 tggcgaatgg tttgataaag cagatcttca aataccgcgc gctgacgctt aaaaccgttg
    62521 gtactgacgt ttgccaggtt gttggcaatg acgtccatat tggtttgctg ggcatccagg
    62581 cccgttttgg cgatccataa tgaacttatc ataaaatgtc ctgtagtgga ttagtgacaa
    62641 agtgcatctg tcttcttgcc ggatgcggcg taaacgcctt atccggccta caagagctcg
    62701 cgaaatcaat aaattgcgat tgccccatag acccgataaa cttgcgcatc gggcaactta
    62761 tacttgtcat gtatcctttc aattaactca tcgacagcag ttggttggct cggcctgcgt
    62821 tatcatcgac gctgctgatc accttcatct gcatttcaaa acgccgcgcg ctggcaatca
    62881 tgtcgctcat tgccgcaacg gcattgacgt tactgccttc cagaacaccc gacatcacac
    62941 gcaaggttgg atctgcctgc agtaccggcc cacgcgtggt ctgggtttct gcgcttaaac
    63001 gaaaaatgcc gtcgtcaccg cgctggactt cgctgcccgt ggctttcacc agtttcagac
    63061 gccccactgg cgcaaccgta tttgccggat cgcccggatt cagcgccgag attgtgccat
    63121 cggcagcaat agtgatttcc gccccttccg gcacagcaat tggcccagcc tcgcctatca
    63181 ccggatgtcc ctgaattgtc agttgcccgg tgggatcaac ctgaatgctg ccattacgcg
    63241 tatacccttc gctaccgtca gcggtctgca cggccagcca gccatcctgc tgcaacgcaa
    63301 cgtccagcgg gcgcgaggtg taatccatct tgccgggcgt catgtctgca cccggcgttg
    63361 acgccgttac cagcgtgcgc gtaggcagtg aaagcccttc caccggcact gcgcgcaagg
    63421 cgtttaactg cgcgcgaaaa ccgggcgttg aggcattggc cagattactg gcggttaccg
    63481 cctgttgatt caacgtctgg ctggctgctc ccatcgcggt ataaattgcg tgatccattg
    63541 agctatcccg tcagcgatta gcgtaagtta accagcgtgt tgaggatctg gtcctgggtt
    63601 ttgatggtct gggcgttaga ctgatagtta cgctgagcaa cgatcatatt gaccagttct
    63661 ttactgagat cgacgttgga cgcttccagc gcaccgttgg tcagggtgcc aaagttgccc
    63721 gtcccggctg tccccaacag cgccacgcca gaagattgcg tcgcagacca gacgttgtcg
    63781 ccttcggatg ccagaccttc gttgttggca aagttcgcca gtacaatctg ccccagcagt
    63841 tgggtttgtt cgttggaata gttgccgaca accgtaccgt catcattgat ttgataactc
    63901 accagatcgc ccggtttgta gccgttctgg gtggttgcca caatgttgtt agcgccggta
    63961 ttttgctgca tggagttgag gaagctcagg ctaaacgtgg cgggttctgc accgttaatt
    64021 gcgccagtgg aaatattcgc tgttggatct gaggtcagaa cgccattggc attaaacacc
    64081 agcgtcattg caggctcagc tgtacctgtt ggatcactgc tatcctgggt gtagacctgc
    64141 cagttattat ccccggtctt cacaaagtag acgctcatgt catgagcatt accctgacta
    64201 tcgaaaacag tcaccgaacc ttttttgtta tagctatccg cattgctagc atcaaatgcg
    64261 ttaacagagg gaagcggatc actggaattc aggttgatct gcatcgacgc cgtggtggta
    64321 gttttcgctg ccatcagggt attcggaatc gaaatattgg tcggattcgc cccttgctga
    64381 atagtcggcg gcgtaccggt tgccgggtaa cccgtcagct gtaaaccttg catattcacc
    64441 aggttacggt tttcatccag cttaaattgt ccgttacggc tgtagaacac cgaaccgttg
    64501 ctgtctacca gacggaaaaa accgttctgg ctgatagcaa cgtccagacc tcgcccggtg
    64561 ttggtggtcg tgccatcggt aaagtcctga gtgataccag caacttttac ccccagtccc
    64621 actttcgaac cggcaaacat atcggcaaaa gaggccgtgc ctgatttaaa gccgtaggtg
    64681 gcagagttgg cgatattgtt gccaataaca tcgaggttgg tggcggcagc gtttaatccg
    64741 ctaaccgctt gagaaaaggc catgactgac tcctgaaatg tgaaggctta aattatctgc
    64801 cgtacttcgt cgagggtggt ggtgccgtaa gtgccgagat ccagcgtatt accgttgttg
    64861 ccgcggatca caccctgcac cagagcaaac tgcagcggct gggcaaccag ttgtgtacca
    64921 ccgttactgg cgctaatcgc tacgttgtaa gaaccgttcg gcgcagtggt gccgtcagtc
    64981 aacgtaccgt cccaggtgaa actgtggact ccggcggtca gttcaccaat atcaatggta
    65041 cggacaaccg caccgttttt atcggtgatg gtggccgtca ctttgtctgc cgcctgttgc
    65101 agctcaacac caaacggcgt ggtcgtggtc acagcccctt cttcactgcc ggttcccgca
    65161 agaacagtgg tgccggggat catcacgccg tgaccgatca ggttactggc ctgtaacgac
    65221 tggctgttat caatctgtcc ggaaatagat ccgagcgtgg tattgagttt ttcaatcccg
    65281 ctgaccgtgc tgatttgagc caattgcgac gtcagctcgt tgttttccat tggattggtc
    65341 gggtcctggt ttttcagctg cgccaccaac aaagtcagaa aactgctttg taaatctgcg
    65401 gcgttgctgc ccgtgagcga actactactg gtggtgctga cgccggtatt tgtcggatcg
    65461 gtggtggtta ccgcaatgga catagttttc tcctttattg accgagcgta agggttttca
    65521 gcatcatgct tttcaccgtg ttgagcactt caacattggc ctgatagctg cgtgacgccg
    65581 acatggtgtt aaccatctct ccgacgacat caacgtttgg cattttcacg tagccctttg
    65641 catccgccag cggattaccc ggttcataaa ccagtttgtc cggggcctga ctttctataa
    65701 catcggcaac ctttacgccg cctgtcgcag cacctggcgc agcgttaacc tgaaatacca
    65761 cctgttttgc ccgatatggc tgtccatcgg gaccggtcac gctatcagca ttcgccagat
    65821 tactggccgc cacgttcagg cgctgggact gagcagttaa cgccgacccg gcgatatcaa
    65881 aaatattcag cagtgccatc cgttaatttc cgctctgtaa aacgttcatc atgcctttga
    65941 tttgcccgct caacgcgcta aggctcatct ggtattgcag gctgttatcg gcaaactggg
    66001 tgcgttcgcg atccatatcg acagtattac catccagcga gggctggtcc ggaatacggt
    66061 attgcagttc tgcggtagga ggcgtcagcg cctgcgccgg aatgtgttgc gttgaggtca
    66121 tcgtcagtgc aaccacgctt gttgcatccc gtccacgttg catgactttt ttaagttcac
    66181 tggcaaaatc gatatcgcgc gcctgataac caggggtatc ggcattggcg atgtttgctg
    66241 ccagcacttc ctgacgctgg gcgcgcagat tgagcgcctc ttgttgaaaa cgtaaggcgg
    66301 cgtcgagctt atcgagcata tctcctccgc aggtatcaaa attcggccat cagcttaaat
    66361 gcctttacgc gcgcgttatc ggcggaataa acgcaaaatg ggtcgctatt tatgccgttg
    66421 atggtcattg cggacaggta caattcacgt tgtagaaatg gctggggggc gaaaatgctg
    66481 gcaataaaac gtagcgtggc gatcatcgcg atactgttca gtccgttaag tgcggcgagc
    66541 aatctcacat cgcaattgca cacctttttt agcgcccagc tcgcgggggt aagtgatgag
    66601 gttcgtgttt ctattcgtac agcgcccaat ctactaccgc cgtgcgagca gccattgctt
    66661 tcgatgagca ataattcccg cctgtggggc aatgtgaatg tgttggcacg ctgcggtaac
    66721 gacaaacgat atttacaggt taatgtgcag gccacaggaa attatgtggt tgccgcaatg
    66781 cccattgtgc ggggaggaaa gctggaagct ggcaatgtca aactgaaacg cggacggctg
    66841 gacaccttgc caccgcgtac ggtgctggat atcaatcaac ttgttgatgc cgttagcctg
    66901 cgcgatctat cacccgatca acctatccag ttaacccagt ttcgccaggc atggcgggta
    66961 aaagcgggac aacgcgtcaa cgtgatcgcc agcggtgatg ggtttagcgc caacgcagaa
    67021 ggtcaggcgc tgaacaatgc tgccgtcgca cagaatgcgc gggtgcgcat ggtatcggga
    67081 caagtggtca gcggcgttgt tgatgcagat gggaatattc ttataaacct gtaagctgtt
    67141 aaagattacc cgtcccttgc cgataaataa gcaacacatg ataaaagcgc cctcaatgag
    67201 gaataaacca tgagtattga tcgcacttcg cctctgaagc ccgtaagcac cgttcaaccg
    67261 cgcgaaacca ctgacgcgcc ggtaacgaac acccgggcgg caaaaacaac cgcctccacc
    67321 agcaccagtg tgacgttaag cgacgcgcaa gcaaaactga tgcaacccgg cagcagtgat
    67381 atcaatcttg aacgtgtcga agcgttaaaa ctagcgattc gtaacggtga actaaaaatg
    67441 gacaccggca aaattgccga tgcgctgatc aacgaagcgc agcaagactt gcagagtaac
    67501 tgatcgtatg acgcgtcttg cagagatcct cgaccagatg tccgctgtgc ttaacgatct
    67561 caaaacggta atggatcaag agcagcaaca tctctctatg gggcagatca acggcagcca
    67621 gttgcaatgg attacagaac aaaaaagctc tctgctggcg acgctggatt acctcgaaca
    67681 gttacgcagg aaagaaccca atgcagcaaa tagcgttgat attagtcaac gctggcagga
    67741 aattactgtg aaaacgcagc aactacgcca aatgaatcaa cataacggct ggttactgga
    67801 aggacagatt gagcgcaatc aacaggcgct ggaaatgttg aaaccgcatc aggaaccgac
    67861 gctatatgga gcgaacggtc agacctcaac aacccatcgc ggcagtaaaa agatttcgat
    67921 ctgaagatta tctccggcct gcactgcagg ccggaatgcc tttttacacc gtccggcggg
    67981 caaactcttt aactttgaag cccagtaccg ccagtgctgc aaagtacgcg gcaatccccg
    68041 ccagcacgac cgccattaaa cgcagtaaac gccagggcat ggtacccaat gaccactccg
    68101 gcatgatatg taacatacct aaaagcacgc cagacatcac cagtaccgcc accaccagac
    68161 gcaacagaaa cgccatccag ccgggttgcg gggtaaagat tttctgctta cgcaactgcc
    68221 agtaaagcag cgaagcattc agacacgccg ccagaccaat agaaagtgac agcccggcat
    68281 gtttcaacgg accgataaac gccaggttca tcaattgcgt taaaatcagc gtaacgatgg
    68341 caattttcac tggcgtttta atgtcctggc gggaataaaa gccaggagcc aacactttca
    68401 ctacaatcag gccaatcaaa cccaccgagt aggcaattaa cgcccgctgg gtcatcagcg
    68461 catcaaaggc ggtaaattta ccgtactgga acagcgaaac ggttaacgga cctgaaagaa
    68521 tgcccaacgc aaccgcactc ggcagcgcca acaggaaaca aagacgcagc ccccagtcca
    68581 tcaaacggtt gtattcatca tgattgccac tggcaaaact tttcgacagc gacggcagca
    68641 aaatggtgcc aagcgccacg cccagtacac cggacggaaa ctccattaag cggtcggcgt
    68701 aatacatcca agacaccgaa ccggaagcaa gaaacgaggc aaaaatggtg ttgatgatta
    68761 aggagatctg gctcacagag acgccaagga tcgccggtcc catctgtttc accacgcgca
    68821 ttgctccggc atcgtggaag ttaatgcgcg gcaggaccag catgccgatc ttcttcaggt
    68881 gcggtagctg atacaccagc tgcaggatgc cgccgaccgt aacagcccac gccagcgcca
    68941 gcaccggtgg gttaaagtac ggtgcggcaa acagcgcgaa accaatcatg ctgatattaa
    69001 gcagtgttgg agcaaacgcc ggaatcgaga agcggttcca cgtattcaga atcgctccca
    69061 ccagcgacgc cagggagatc agcaagatat agggaaaggt tatctttagt agctggctgg
    69121 tcagggcaaa tttgtcggca gtgtcagcga agcctggcgc ggtcaccatg atcacccacg
    69181 gtgcagcgag catgccagcg accgtcacaa ccgccagcgc gaatgtcagc aggccagaaa
    69241 cataagagac aaagacccgc gtggcgtctt caccctgctt gcttttatat tctgccagaa
    69301 tcggtacaaa tgcctgggaa aatgcccctt cggcaaagat acggcgcaac aagttaggaa
    69361 gtttaaaagc gacgaaaaag gcgtcggttg ccatccctgc gccaaagatt ctggcgacaa
    69421 ttgcgtcacg tgcgaagcca agcacacgcg aaaacatggt catcgagctg acggcggcca
    69481 gcgattttaa taaattcatc ggtgttctaa tccagaccca cgggcaaaac gcctgcaaag
    69541 caggcgctga aagaggcgaa tagtctacct ggattatggt gaattgctac cgccagatgt
    69601 tacagggttt attcactcat cgcatcgcgc cagatcttgt caacgatacg ttgcgcccgc
    69661 actgcctgtt cgccggcggt ttgcggaact gtctgatttt gcacgcattc aatgaagtgc
    69721 cgcgcacagc cgacaaatcc acgttgctca agcgtactct gccaaccagg aatcggttta
    69781 tgcactacgc cctgcccgcg ctcctcacgc cattcgcgca tatccgtaat gtcgatgagc
    69841 gcaccgtcag tcacggcctg cacggtttca cgctggcttc cggcacggcg atgcatacag
    69901 gtggtgatct gcaaaggacc ggccgaaaaa tggtgctcgg caaacagcat ttcgccagcg
    69961 tcgttagtca gtagcgtacc gccatccaga gaggctttgc cgcccgacaa ccacagcgcg
    70021 gtatccacca catgcagata atcatccagc aacgtgaaat aaagatcgtg tggcccgacg
    70081 ctattgctac gatgtttatc cattcttagc gaggctgcgg tggcgagttg cgtttttaac
    70141 tctccgtaga gtggtgcgaa acgacggtta aaaccgacca tcaacgtcag ttttttccgc
    70201 gccgccagtt ccaccaaccg ttcagcatcg cgcagatttt ctgccagcgg tttatcgaca
    70261 cagacatgta cccccgcatt gagtaacgta ctgaccacgt caaagtggct ggcggtgctg
    70321 gaatgcacaa aaaccgcatc gcaactggcg gcaaggctgg ataacgaatc ggcataagga
    70381 atgcgccagc tttcacaaat tggcagggct ttcgcgcgcg taggcgacca ggctccttgt
    70441 aacatccagt cagacgctgc cgccagcacc ggtaaccacg ctttttgcgc aatgccgcct
    70501 aatcccacta cgccgatacg taattttttc actttaatct cccagatggg caagcaacga
    70561 atcaagacgc tgtttcagtt ctgccacttc gatttccagg gcttcgacgc gagcctgtaa
    70621 atcaccgtca accgcgttcg acatatccat caccgccggc tgatcttcaa cctcaccact
    70681 gaaaagatgc atgtagcggc tttcacgttt acccggttcg cgggccagac gcaccacaaa
    70741 aggaccatct tcgcgatttg ccagttgttc cagcgtcgac tccacttccg ccatatcgct
    70801 gaattcatac attcgcgcgg cgcggctgcg cagttcaccc ggcgtctggg caccacgcaa
    70861 taacaacgtg gtgattaacg ccacttctgc ggcgctcagt ttcagatcgc caaattctga
    70921 attacaaaaa cgttgctcat atttggtgac ccgattacca aaaccgctca ctgtgcgtag
    70981 ataatgacgt ttgaccagat tatccagctg ttcctgcact tcggattcac tcagattcat
    71041 gaccggttca cggttcgttt tctgattaca ggccgtgact acaccattga ctgagagcgg
    71101 gtattgctcc ggcgtggtca cctgcttttc cagcaagcaa ccaatcacgc gagcttcaag
    71161 ggcagtaagt tgatatttca tgagattctc cttagcggcc gggcgtccag tctggggtag
    71221 ttaatgccgt cagtacgtga tcgcgccatt gtccatcaat caacagatag tctttcgcat
    71281 agccttcttt ttcaaaaccc agtcgcgcca gtaaatcacc gctgcgttta ttgtgcggca
    71341 tataattagc cataatgcga tgaatatgtt gggtgcgctg catataacga atggctgcgg
    71401 tcagggcttc aaacatgagt cctttgccct gccatttttg cccaatcgaa taaccgagat
    71461 agcaggcatg aaaagagcca cgaacaacat tggaaaaatt ggcaacgcca ataatctctt
    71521 tttcgtccgg gtcgaataag ccaaagtaga aagctgaacc ttgtttatga aattcgttaa
    71581 tcatccctag cctggcctgc cagcctgatg gataacagtg gctttcgtcg cgcactggct
    71641 cccagggctt gaggaaatgg cgattctctg cgtaataatc cgccagacgc caggcatcac
    71701 gatcatgcac cagacgcacg accagtcggt ctgtggttaa gcgcactttt ggcacgttac
    71761 tgcgatagcc aaacattcta tacctactcc ttcccgtaac gtctttaata cgctgtaagt
    71821 attactatac ctgcgcaacc agaccctgtg aaaagtccag aatgccattt tctgcattat
    71881 tcacatattt gataaaaact ctctatcaaa gcgacgtttt gataaaaaaa tattgtcccc
    71941 gaagtgatgg gaaaagtctt agcggcaatg cagaatagaa agtgctcatc ttttatttcc
    72001 cgggagggga aatgtcccgc gtgtcgcagg ccaggaacct gggtaaatat ttcctgctca
    72061 tcgataatat gctggtcgtg ctggggttct ttgttgtctt cccgctgatc tctatccgct
    72121 tcgttgatca aatgggctgg gccgccgtca tggtcggtat tgccctcggc ctacgccagt
    72181 ttattcagca aggtctgggt attttcggcg gagcaattgc cgaccgcttt ggtgccaaac
    72241 cgatgattgt taccggtatg ctgatgcgtg ccgccggatt cgccacaatg ggtatcgccc
    72301 acgaaccgtg gctactgtgg ttttcatgcc tgctttcggg actcggcggc acgttgtttg
    72361 atcctccgcg ttcggcgctg gtggtgaaat taatccgtcc acagcagcgt ggtcgttttt
    72421 tctcgctgtt gatgatgcag gacagtgccg gtgcggtcat tggcgcattg ttggggagct
    72481 ggctgttgca atacgacttt cgcctggtct gcgccacagg ggcagttttg tttgtgctgt
    72541 gtgcagcgtt taatgcgtgg ttgttaccgg catggaaact ctccaccgta cgcacgcccg
    72601 ttcgcgaagg catgacccgc gtgatgcgcg acaagcgttt tgtcacctat gtactgacgc
    72661 tggcgggtta ctacatgctg gctgtacaag tgatgctgat gctgccaatt atggtcaacg
    72721 acgtggcggg cgcgccctct gccgttaaat ggatgtatgc cattgaagcg tgtctgtcgt
    72781 taacgttgct ctaccctatc gcccgctgga gtgaaaagca ttttcgtctg gaacaccggt
    72841 tgatggctgg gctattgata atgtcattaa gcatgatgcc ggtgggcatg gtcagcggtc
    72901 tgcaacaact tttcaccctg atttgtctgt tttatatcgg gtcaatcatt gccgagcctg
    72961 cgcgtgaaac cttaagtgct tcgctggcgg acgcaagagc tcgcggcagc tatatggggt
    73021 ttagccgtct gggtctggcg attggcggcg ctattggtta catcggtggc ggctggctgt
    73081 ttgacctggg caaatcggcg caccagccag agcttccgtg gatgatgctg ggcattattg
    73141 gcatcttcac tttccttgcg ctgggttggc agtttagcca gaaacgcgcc gcgcgtcgtt
    73201 tgctggaacg cgacgcctga tttgatactc ccccctgact cctccatact gaaagtgaac
    73261 tgacagccaa atggaggagt cacgtgaagc tatacattta cgatcactgc ccttactgcc
    73321 tcaaagcccg catgattttc ggcctgaaaa atatccccat cgaattacat gttctgctca
    73381 acgacgacgc agaaacaccc acccggatgg tcggtcaaaa acaggttccc attctgcaaa
    73441 aagatgacag ccgctatatg ccagaaagca tggacatcgt tcactatgtc gataaactcg
    73501 acggcaaacc gttactgacc ggcaaacgtt cccctgccat tgaagagtgg ctgcgcaagg
    73561 tcaatggcta cgccaacaaa ctgctgttgc cgcgttttgc caaatcggca tttgatgagt
    73621 tttctactcc tgccgcgcgc aaatatttcg tcgacaagaa agaggctagc gcgggtaatt
    73681 ttgccgacct gctggcccac tctgacggtc tgattaagaa tatcagcgat gatttacgtg
    73741 cgctggacaa actgatcgtc aaaccgaacg ccgtgaatgg cgaactttcg gaagatgata
    73801 ttcagctatt tccgctcctg cgtaatctga cgctggtagc cggaatcaac tggccaagcc
    73861 gcgttgctga ttaccgcgat aatatggcga aacagacaca aatcaatttg ttatcatcaa
    73921 tggcgattta atcctgaccc gccgcggcag cctgtattcg cgggttgccg ccgttttcag
    73981 ttttttctgc tggcgcgttt atcgctggtg acacatgctg cgttgtgtca ggatattgac
    74041 gcagatttca tgtcgaggac taccatgaac aagtttttat ttgccgctgc attgatcgtc
    74101 agtggcctgc tcgttggctg taatcaactc acccaataca ccatcaccga acaagaaatt
    74161 aaccagtcgc ttgcgaaaca taataatttc tcaaaagata tcggtttacc cggcgtggct
    74221 gacgcccata ttgttctgac aaacctgacc agccaaattg gtcgcgaaga gccgaataag
    74281 gttaccctaa ccggagacgc caatctggac atgaactccc tgttcggtag ccagaaagcg
    74341 accatgaaac tgaagctgaa agcgctgccc gtgtttgata aagaaaaagg cgctatcttc
    74401 ctgaaagaga tggaagtggt cgatgcgacg gtacaaccgg aaaaaatgca aacggtgatg
    74461 caaacgttgc ttccctattt aaaccaggca ttacgcaatt actttaacca gcaacctgct
    74521 tacgtcctgc gcgaagatgg cagccagggc gaagcaatgg cgaagaaact ggcgaaaggc
    74581 attgaagtga agccaggcga aattgtcatt ccgtttactg attaatcacg agggcgcatt
    74641 cgcgcccttt atttttcgtg caaaggaaaa cgtttccgct tatcctttgt gtccggcaaa
    74701 aacatccctt cagccggagc atagagatta atgactgcac catcccaggt attaaagatc
    74761 cgccgcccag acgactggca ccttcacctc cgcgatggcg acatgttaaa aactgtcgtg
    74821 ccgtatacca gcgaaattta tggacgggct atcgtaatgc ccaatctggc tccgcccgtg
    74881 accaccgttg aggctgccgt ggcttatcgc cagcgtattc ttgacgccgt acctgccggg
    74941 cacgatttca ccccgctgat gacctgttat ttgacagatt cactagaccc taatgagctt
    75001 gagcgtggat ttaacgaagg cgtgttcacc gctgccaaac tttacccggc aaacgcaacc
    75061 actaactcca gtcacggcgt gacgtcagtt gacgcaatca tgccggtact tgagcgcatg
    75121 gaaaaaatcg gtatgccgtt actggtgcat ggtgaagtga cacatgcaga tatcgacatt
    75181 tttgatcgtg aagcgcgctt tattgaaagc gtgatggaac cgctacgcca gcgtctgact
    75241 gcgctgaaag tcgtttttga gcacatcacc accaaagacg ctgccgacta tgtccgtgac
    75301 ggaaatgaac ggctggctgc caccatcact ccgcagcatc tgatgtttaa ccgcaaccat
    75361 atgctggttg gtggcgtgcg tccgcacctg tattgtctac ccatcctcaa acgcaatatc
    75421 caccaacagg cattgcgtga actggtcgcc agcggtttta atcgcgtatt cctcggtacg
    75481 gattctgcgc cgcatgcacg tcatcgcaaa gagagcagct gcggctgcgc gggctgcttc
    75541 aacgccccaa ccgcgctggg cagttacgct accgtctttg aagagatgaa tgctctgcag
    75601 cactttgaag cattctgttc tgtaaacggc ccacagttct atggcttgcc ggtcaacgac
    75661 acattcatcg aactggtccg caaagagcaa caggttgctg aaagcatcgc attgactgat
    75721 gacacgctgg tgccattcct cgccggggaa acggtacgct ggtccgttaa acaataaaaa
    75781 tcgtagcttc ctgttgtcat taggttattt taactgtata aataaccagt atattcaaca
    75841 gggggctatt atgcgaattg aagtcaccat agcgaaaact tctctattgc cagctggggc
    75901 tattgacgcc ctggctggcg aactttcccg ccgtattcag tatgcgtttc ctgataatga
    75961 aggccacgta tcggtacgtt atgccgcagc gaataattta tcggttattg gcgcgacaaa
    76021 agaagataaa cagcgcatta gcgaaattct ccaggaaacg tgggaaagcg ccgatgactg
    76081 gtttgtcagc gaataatatg cagtgatttt tgtttttgcc gggtcgcccc ggctttttta
    76141 ttttacttg
//
LOCUS       DAZGQL010000009        76067 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.009, whole genome shotgun sequence.
ACCESSION   DAZGQL010000009 DAZGQL010000000
VERSION     DAZGQL010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 76067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 76067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..76067
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.009"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(157..1104)
                     /gene="dusC"
                     /locus_tag="ACHKIR_000719"
     CDS             complement(157..1104)
                     /gene="dusC"
                     /locus_tag="ACHKIR_000719"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HFO9988509.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPAGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMTISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
     gene            complement(1166..2359)
                     /locus_tag="ACHKIR_000720"
     CDS             complement(1166..2359)
                     /locus_tag="ACHKIR_000720"
                     /EC_number="1.14.13.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybenzoate 6-monooxygenase"
                     /protein_id="HFO9988510.1"
                     /translation="MTKVTRAVIVGGGIGGAATALSLARQGIKVVLLEKAHEIGEIGA
                     GIQLGPNAFSALDSLGVGEIARQRAVFTDHITMMDAVNAEEVVRIETGQAFRDHFGGP
                     YAVIHRVDIHASVWEAVLTHPDVEYHTSTNVVDIRETEDDVTVFDDRGNSWIGDILIG
                     CDGVKSVVRQSLLGDAPRVTGHVVYRAVIDCADMPEDLRINAPVLWAGPCCHLVHYPL
                     RGGKQYNLVVTFHSRQQEEWGVKDGSKEEVLSYFEGIHPRPRQMLDKPTSWRRWSTAD
                     REPVAKWGTKRITLVGDAAHPVAQYMAQGACMALEDAVTLGKALAQCEGDAAQAFALY
                     ESVRIPRTARIVWSTREMGRVYHAAGVERQVRNLLWKGKSQAEFYRGMEWLYGWKEDN
                     CLQPR"
     gene            complement(2374..3018)
                     /gene="maiA"
                     /locus_tag="ACHKIR_000721"
     CDS             complement(2374..3018)
                     /gene="maiA"
                     /locus_tag="ACHKIR_000721"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311055.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HFO9988511.1"
                     /translation="MKLYSFFNSSASYRVRIALALKGIDYQTEGVNIRIGQQNELAYR
                     RMNPVGLVPTLLTDEGESLGQSLAIIDWLESHYPQVPLMPQEEPARNKVLEIVYAIAC
                     DIHPLNNLRVLRYLTEELNVSEEDKKRWYAHWIQQGLSAVEQLLRQSQSGQFCVGETP
                     TLADCCLVPQWANALRMNCDLSGYPRCKAVYDACTQLPAFIAAAPENQQDKISA"
     gene            complement(3026..3727)
                     /locus_tag="ACHKIR_000722"
     CDS             complement(3026..3727)
                     /locus_tag="ACHKIR_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFO9988512.1"
                     /translation="MTQYVFEPQAPVTVPVVGSDEQFPVRRVYCVGRNYAAHAREMGF
                     DPDREPPFFFCKPADAIVPVAAGETLALPYPAQTDNYHYEIELVVAIGKKGSDIPLEN
                     AHEYVWGYATGLDMTRRDRQMEMRQMGRPWEIGKAFDLSAPIAPLHKASETGGIDNAP
                     IWLQVNGEDHQRSDIRHLIWSVNETISYLSGFFELQPGDLIFTGTPEGVGSVVKGDVI
                     TGNVEGLTPIAVKIV"
     gene            complement(3743..4771)
                     /gene="gtdA"
                     /locus_tag="ACHKIR_000723"
     CDS             complement(3743..4771)
                     /gene="gtdA"
                     /locus_tag="ACHKIR_000723"
                     /EC_number="1.13.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047922 - gentisate 1,2-dioxygenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gentisate 1,2-dioxygenase"
                     /protein_id="HFO9988513.1"
                     /translation="MTDNNQNSREQFYQHISGQNLTPLWESLHHLVPKTPNANCAPAY
                     WNYQEIRPLLLESGSLIGAKEAVRRVLVLENPALRGQSSITATLYAGLQLIMPGEVAP
                     SHRHNQSALRFIVEGKGAFTAVDGERTSMNEGDFILTPQWRWHDHGNPGDEPVIWLDG
                     LDLPLVNTLGCGFAEDYPEEQQPVTRKEGDYLPRYAANMLPLRHQTGNSSPIFNYRYD
                     RSREVLHDLTRLGDADEWDGYKMRYVNPVTGGYPMPSMGAFLQLLPKGFASRVARTTD
                     STIYHVVEGSGQVTIGNETFSFSAKDIFVVPTWHGVSFQTTQDTVLFSFSDRPVQEAL
                     GLFREARY"
     gene            complement(4783..6141)
                     /locus_tag="ACHKIR_000724"
     CDS             complement(4783..6141)
                     /locus_tag="ACHKIR_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9988514.1"
                     /translation="MTQRRDLQALIDAAPVGKMQWRVIICCFLVVMLDGFDTAAIGFI
                     APDIRTHWQLTAGDLAPLFGAGLLGLTAGALLCGPLSDRFGRKRVIELCVFLFGALSL
                     ASAFSPDLQTLVFLRFLTGLGLGGAMPNTITMTSEYLPARRRGALVTLMFCGFTLGSA
                     FGGIVSAQLVPVIGWHGILVLGGVLPLMLFVALLVVLPESPRWQVRRQLPQAVIAKTV
                     SAIIRERYVDTHFYLIESASVTKGSIRQLFMGRQLPITLMLWVVFFMSLLIIYLLSSW
                     MPTLLNHRGIDLQHASWVTAAFQIGGTLGALALGVLMDKFNPFRVLALSYAIGAVCIV
                     MIGLSQDGLWLMALAIFGTGIGISGSQVGLNALTATLYPTQSRATGVSWSNAVGRCGA
                     IVGSLSGGVMMAMNFSFDTLFFIIAVPAAISAVMLTQLITVVRQSTSVPDSLPHAGVV
                     NE"
     gene            6268..7176
                     /locus_tag="ACHKIR_000725"
     CDS             6268..7176
                     /locus_tag="ACHKIR_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO9988515.1"
                     /translation="MANWAQKLKLHHLQMLVALGEQGNLTHVARMMNITQPALSKWLS
                     QLEDEIGLTLFERHSKGLRPTEGGKLLLQHAQRLINDMERSQHELARFKQGGLVGSLK
                     IGCSPVATDCVSQAILHLLKEMPTLHLNIEEKVMTPLLQDLLAGSVDVVVGRIGGRAL
                     QLPLNYRVLYTEPVCFVARPEHPLAKYATLSWNDLANWRWIVWPTGTPIRISIDNALV
                     DHGVMLPENTIESASMNVSTNLLQSSDMISILSLRLAQRYASQGQLVILNLPKIEQKG
                     SVGVFWRNNETPSYALSQFLHFLQQN"
     gene            7307..7705
                     /gene="yohJ"
                     /locus_tag="ACHKIR_000726"
     CDS             7307..7705
                     /gene="yohJ"
                     /locus_tag="ACHKIR_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFO9988516.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            7702..8397
                     /gene="yohK"
                     /locus_tag="ACHKIR_000727"
     CDS             7702..8397
                     /gene="yohK"
                     /locus_tag="ACHKIR_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HFO9988517.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            8527..9411
                     /gene="cdd"
                     /locus_tag="ACHKIR_000728"
     CDS             8527..9411
                     /gene="cdd"
                     /locus_tag="ACHKIR_000728"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFO9988518.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALVAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHNIDRVLLA"
     gene            9561..10280
                     /gene="sanA"
                     /locus_tag="ACHKIR_000729"
     CDS             9561..10280
                     /gene="sanA"
                     /locus_tag="ACHKIR_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HFO9988519.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            10283..10522
                     /gene="yeiS"
                     /locus_tag="ACHKIR_000730"
     CDS             10283..10522
                     /gene="yeiS"
                     /locus_tag="ACHKIR_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HFO9988520.1"
                     /translation="MDVQQFFVVAVFFLIPVFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            10841..12079
                     /gene="preT"
                     /locus_tag="ACHKIR_000731"
     CDS             10841..12079
                     /gene="preT"
                     /locus_tag="ACHKIR_000731"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HFO9988521.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDTPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAQPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGNTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLQRARQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLPGELT
                     MAADKIILAVGQHARLDAFAELEPQRNTIKTQNYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLKGACSC"
     gene            12073..13308
                     /gene="preA"
                     /locus_tag="ACHKIR_000732"
     CDS             12073..13308
                     /gene="preA"
                     /locus_tag="ACHKIR_000732"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HFO9988522.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEDNLAALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPHINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IDLGEVKFKKGEKEHPVTL"
     gene            complement(13379..14389)
                     /gene="mglC"
                     /locus_tag="ACHKIR_000733"
     CDS             complement(13379..14389)
                     /gene="mglC"
                     /locus_tag="ACHKIR_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HFO9988523.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(14405..15925)
                     /gene="mglA"
                     /locus_tag="ACHKIR_000734"
     CDS             complement(14405..15925)
                     /gene="mglA"
                     /locus_tag="ACHKIR_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043211 - ABC-type carbohydrate
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HFO9988524.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKKINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            complement(15986..16984)
                     /gene="mglB"
                     /locus_tag="ACHKIR_000735"
     CDS             complement(15986..16984)
                     /gene="mglB"
                     /locus_tag="ACHKIR_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HFO9988525.1"
                     /translation="MNKKVLTLFAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            complement(17264..18304)
                     /gene="galS"
                     /locus_tag="ACHKIR_000736"
     CDS             complement(17264..18304)
                     /gene="galS"
                     /locus_tag="ACHKIR_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HFO9988526.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNLAM"
     gene            complement(18446..19603)
                     /gene="yeiB"
                     /locus_tag="ACHKIR_000737"
     CDS             complement(18446..19603)
                     /gene="yeiB"
                     /locus_tag="ACHKIR_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein YeiB"
                     /protein_id="HFO9988527.1"
                     /translation="MGRNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTTLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGPARSKTSR"
     gene            complement(19620..20288)
                     /gene="folE"
                     /locus_tag="ACHKIR_000738"
     CDS             complement(19620..20288)
                     /gene="folE"
                     /locus_tag="ACHKIR_000738"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HFO9988528.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            20546..21382
                     /gene="yeiG"
                     /locus_tag="ACHKIR_000739"
     CDS             20546..21382
                     /gene="yeiG"
                     /locus_tag="ACHKIR_000739"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFO9988529.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEQVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSSYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            complement(21414..23393)
                     /gene="cirA"
                     /locus_tag="ACHKIR_000740"
                     /pseudo
     CDS             complement(21414..23393)
                     /gene="cirA"
                     /locus_tag="ACHKIR_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311074.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
     gene            complement(23686..25155)
                     /gene="lysP"
                     /locus_tag="ACHKIR_000741"
     CDS             complement(23686..25155)
                     /gene="lysP"
                     /locus_tag="ACHKIR_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HFO9988530.1"
                     /translation="MVSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAVS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            complement(25360..26241)
                     /gene="yieE"
                     /locus_tag="ACHKIR_000742"
     CDS             complement(25360..26241)
                     /gene="yieE"
                     /locus_tag="ACHKIR_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YeiE"
                     /protein_id="HFO9988531.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            26340..27389
                     /gene="yeiH"
                     /locus_tag="ACHKIR_000743"
     CDS             26340..27389
                     /gene="yeiH"
                     /locus_tag="ACHKIR_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HFO9988532.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            27463..28320
                     /gene="nfo"
                     /locus_tag="ACHKIR_000744"
     CDS             27463..28320
                     /gene="nfo"
                     /locus_tag="ACHKIR_000744"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFO9988533.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVAEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTSAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            28323..29411
                     /gene="yeiI"
                     /locus_tag="ACHKIR_000745"
     CDS             28323..29411
                     /gene="yeiI"
                     /locus_tag="ACHKIR_000745"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFO9988534.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKGKGYILTEQEYCVVVGTINMDIRGMADIRYPQAASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDQT
                     VLAINDTHLLDQLTPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLADEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGNNFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(29467..30717)
                     /gene="nupX"
                     /locus_tag="ACHKIR_000746"
     CDS             complement(29467..30717)
                     /gene="nupX"
                     /locus_tag="ACHKIR_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFO9988535.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKSFINRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(30817..31758)
                     /gene="rihB"
                     /locus_tag="ACHKIR_000747"
     CDS             complement(30817..31758)
                     /gene="rihB"
                     /locus_tag="ACHKIR_000747"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HFO9988536.1"
                     /translation="MEKRKIILDCDPGHDDAIAIMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGELFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            31888..32586
                     /gene="yeiL"
                     /locus_tag="ACHKIR_000748"
     CDS             31888..32586
                     /gene="yeiL"
                     /locus_tag="ACHKIR_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HFO9988537.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFSTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            complement(32654..33904)
                     /locus_tag="ACHKIR_000749"
     CDS             complement(32654..33904)
                     /locus_tag="ACHKIR_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFO9988538.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(33998..34936)
                     /gene="psuG"
                     /locus_tag="ACHKIR_000750"
     CDS             complement(33998..34936)
                     /gene="psuG"
                     /locus_tag="ACHKIR_000750"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HFO9988539.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELAHTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEHTINAAIDQAVAEAEAQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            complement(34924..35865)
                     /gene="psuK"
                     /locus_tag="ACHKIR_000751"
     CDS             complement(34924..35865)
                     /gene="psuK"
                     /locus_tag="ACHKIR_000751"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HFO9988540.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHGEFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNQIHTLKPNRLEAETLSGIALSGREDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGESGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            complement(36289..37980)
                     /gene="fruA"
                     /locus_tag="ACHKIR_000752"
     CDS             complement(36289..37980)
                     /gene="fruA"
                     /locus_tag="ACHKIR_000752"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HFO9988541.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVVATAPVATSG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATTE
                     GKKEGAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYISFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            complement(37997..38935)
                     /gene="fruK"
                     /locus_tag="ACHKIR_000753"
     CDS             complement(37997..38935)
                     /gene="fruK"
                     /locus_tag="ACHKIR_000753"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFO9988542.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFDVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(38935..40065)
                     /gene="fruB"
                     /locus_tag="ACHKIR_000754"
     CDS             complement(38935..40065)
                     /gene="fruB"
                     /locus_tag="ACHKIR_000754"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HFO9988543.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEAGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            40433..41248
                     /locus_tag="ACHKIR_000755"
                     /pseudo
     CDS             40433..41248
                     /locus_tag="ACHKIR_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            41237..41800
                     /locus_tag="ACHKIR_000756"
     CDS             41237..41800
                     /locus_tag="ACHKIR_000756"
                     /inference="COORDINATES: protein motif:HMM:NF019303.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFO9988544.1"
                     /translation="MLIAADIIVANKSDRATPESEQALQRWWQQNGGDRQLIHSEHGK
                     IDGHLLDLPRRNLAELPASAAHSHQHGVKKGLAALSLPEHQRWRRSLNSGQGYQACGW
                     IFDADTVFDTIGILEWARLAPVERVKGVMRIPEGLVRINRQGDDLHIETQNVAPPDSR
                     IELISSSEADWNALQSALLKLRLATTA"
     gene            41839..42552
                     /gene="lpxT"
                     /locus_tag="ACHKIR_000757"
     CDS             41839..42552
                     /gene="lpxT"
                     /locus_tag="ACHKIR_000757"
                     /EC_number="2.7.4.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HFO9988545.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIIGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            42964..43530
                     /gene="mepS"
                     /locus_tag="ACHKIR_000758"
     CDS             42964..43530
                     /gene="mepS"
                     /locus_tag="ACHKIR_000758"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HFO9988546.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            43711..45267
                     /gene="pdeN"
                     /locus_tag="ACHKIR_000759"
     CDS             43711..45267
                     /gene="pdeN"
                     /locus_tag="ACHKIR_000759"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFO9988547.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLITDVQKYLDTYFTDLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLKELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTFVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVVYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAEAQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPAHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEREATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            45349..47163
                     /gene="yejA"
                     /locus_tag="ACHKIR_000760"
     CDS             45349..47163
                     /gene="yejA"
                     /locus_tag="ACHKIR_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFO9988548.1"
                     /translation="MIVRILLLFIALFTFGAQAQTIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNAGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYRITSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRLEND
                     AKNWVTRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPSSSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRVWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKATKLPSARQQGE"
     gene            47164..48258
                     /gene="yejB"
                     /locus_tag="ACHKIR_000761"
     CDS             47164..48258
                     /gene="yejB"
                     /locus_tag="ACHKIR_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HFO9988549.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNTGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            48258..49283
                     /gene="yejE"
                     /locus_tag="ACHKIR_000762"
     CDS             48258..49283
                     /gene="yejE"
                     /locus_tag="ACHKIR_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO9988550.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            49285..50874
                     /gene="yejF"
                     /locus_tag="ACHKIR_000763"
     CDS             49285..50874
                     /gene="yejF"
                     /locus_tag="ACHKIR_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HFO9988551.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LINSQGSIVFDGKPLQNLNRRQLLPIRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFAAPQQEYTRQLLALS"
     gene            complement(50878..51222)
                     /gene="yejG"
                     /locus_tag="ACHKIR_000764"
     CDS             complement(50878..51222)
                     /gene="yejG"
                     /locus_tag="ACHKIR_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejG family protein"
                     /protein_id="HFO9988552.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(51556..52746)
                     /gene="bcr"
                     /locus_tag="ACHKIR_000765"
     CDS             complement(51556..52746)
                     /gene="bcr"
                     /locus_tag="ACHKIR_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HFO9988553.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVW
                     LSWHYIFWILAVAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNILFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(52774..53469)
                     /gene="rsuA"
                     /locus_tag="ACHKIR_000766"
     CDS             complement(52774..53469)
                     /gene="rsuA"
                     /locus_tag="ACHKIR_000766"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HFO9988554.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            53619..55379
                     /gene="radD"
                     /locus_tag="ACHKIR_000767"
     CDS             53619..55379
                     /gene="radD"
                     /locus_tag="ACHKIR_000767"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFO9988555.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAVTRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            55504..55788
                     /gene="rplY"
                     /locus_tag="ACHKIR_000768"
     CDS             55504..55788
                     /gene="rplY"
                     /locus_tag="ACHKIR_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HFO9988556.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAVELDH
                     DKVMNMQVKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLLHIDFVRA"
     gene            complement(55927..56934)
                     /gene="yejK"
                     /locus_tag="ACHKIR_000769"
     CDS             complement(55927..56934)
                     /gene="yejK"
                     /locus_tag="ACHKIR_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HFO9988557.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            57116..57343
                     /gene="yejL"
                     /locus_tag="ACHKIR_000770"
     CDS             57116..57343
                     /gene="yejL"
                     /locus_tag="ACHKIR_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HFO9988558.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            57363..59123
                     /gene="yejM"
                     /locus_tag="ACHKIR_000771"
     CDS             57363..59123
                     /gene="yejM"
                     /locus_tag="ACHKIR_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS biosynthesis-modulating metalloenzyme YejM"
                     /protein_id="HFO9988559.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            59198..59274
                     /locus_tag="ACHKIR_000772"
     tRNA            59198..59274
                     /locus_tag="ACHKIR_000772"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:59232..59234,aa:Pro,seq:ggg)
     gene            complement(59400..60159)
                     /locus_tag="ACHKIR_000773"
                     /pseudo
     CDS             complement(59400..60159)
                     /locus_tag="ACHKIR_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            60479..61126
                     /gene="narP"
                     /locus_tag="ACHKIR_000774"
     CDS             60479..61126
                     /gene="narP"
                     /locus_tag="ACHKIR_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HFO9988560.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(61161..62213)
                     /gene="ccmH"
                     /locus_tag="ACHKIR_000775"
     CDS             complement(61161..62213)
                     /gene="ccmH"
                     /locus_tag="ACHKIR_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HFO9988561.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKAAPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(62210..62767)
                     /gene="dsbE"
                     /locus_tag="ACHKIR_000776"
     CDS             complement(62210..62767)
                     /gene="dsbE"
                     /locus_tag="ACHKIR_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HFO9988562.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKL
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            complement(62764..64707)
                     /gene="ccmF"
                     /locus_tag="ACHKIR_000777"
     CDS             complement(62764..64707)
                     /gene="ccmF"
                     /locus_tag="ACHKIR_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HFO9988563.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAMLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTRNGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVNPQKTAPEAV"
     gene            complement(64704..65183)
                     /gene="ccmE"
                     /locus_tag="ACHKIR_000778"
     CDS             complement(64704..65183)
                     /gene="ccmE"
                     /locus_tag="ACHKIR_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HFO9988564.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            complement(65180..65389)
                     /gene="ccmD"
                     /locus_tag="ACHKIR_000779"
     CDS             complement(65180..65389)
                     /gene="ccmD"
                     /locus_tag="ACHKIR_000779"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HFO9988565.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVILVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            complement(65386..66123)
                     /gene="ccmC"
                     /locus_tag="ACHKIR_000780"
     CDS             complement(65386..66123)
                     /gene="ccmC"
                     /locus_tag="ACHKIR_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HFO9988566.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            complement(66165..66827)
                     /gene="ccmB"
                     /locus_tag="ACHKIR_000781"
     CDS             complement(66165..66827)
                     /gene="ccmB"
                     /locus_tag="ACHKIR_000781"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HFO9988567.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            complement(66824..67447)
                     /gene="ccmA"
                     /locus_tag="ACHKIR_000782"
     CDS             complement(66824..67447)
                     /gene="ccmA"
                     /locus_tag="ACHKIR_000782"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HFO9988568.1"
                     /translation="MGMLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTT
                     LLRLLTGLSRPDAGDVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHR
                     DGDTAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAID
                     VNGVDRLTQRMAQHTEQGGIVILTTHQPLNVAENKIRRISLTQTRGE"
     gene            complement(67460..68062)
                     /gene="napC"
                     /locus_tag="ACHKIR_000783"
     CDS             complement(67460..68062)
                     /gene="napC"
                     /locus_tag="ACHKIR_000783"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HFO9988569.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            complement(68072..68521)
                     /gene="napB"
                     /locus_tag="ACHKIR_000784"
     CDS             complement(68072..68521)
                     /gene="napB"
                     /locus_tag="ACHKIR_000784"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HFO9988570.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            complement(68518..69381)
                     /gene="napH"
                     /locus_tag="ACHKIR_000785"
     CDS             complement(68518..69381)
                     /gene="napH"
                     /locus_tag="ACHKIR_000785"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HFO9988571.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPASVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILLGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AATDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            complement(69368..70063)
                     /gene="napG"
                     /locus_tag="ACHKIR_000786"
     CDS             complement(69368..70063)
                     /gene="napG"
                     /locus_tag="ACHKIR_000786"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HFO9988572.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            complement(70070..72556)
                     /gene="napA"
                     /locus_tag="ACHKIR_000787"
     CDS             complement(70070..72556)
                     /gene="napA"
                     /locus_tag="ACHKIR_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HFO9988573.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGSNMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPLSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            complement(72553..72816)
                     /gene="napD"
                     /locus_tag="ACHKIR_000788"
     CDS             complement(72553..72816)
                     /gene="napD"
                     /locus_tag="ACHKIR_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HFO9988574.1"
                     /translation="MYTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            complement(72806..73300)
                     /gene="napF"
                     /locus_tag="ACHKIR_000789"
     CDS             complement(72806..73300)
                     /gene="napF"
                     /locus_tag="ACHKIR_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HFO9988575.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLLFTIGEAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNACGACAASCPVSAITAEY
                     LHVH"
     gene            73409..73573
                     /locus_tag="ACHKIR_000790"
     CDS             73409..73573
                     /locus_tag="ACHKIR_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9988576.1"
                     /translation="MHTPIGVKPVAGSKEWQEAWQKRAFAHISNGYKHIYIAINSPEI
                     FLLVCFLIRI"
     gene            73687..74196
                     /gene="eco"
                     /locus_tag="ACHKIR_000791"
     CDS             73687..74196
                     /gene="eco"
                     /locus_tag="ACHKIR_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004867 - serine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HFO9988577.1"
                     /translation="MKKESKKMKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAE
                     KGMKRQVIQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFD
                     KVSSPVSTMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAE
                     EKIDNAVVR"
     gene            complement(74346..75992)
                     /gene="mqo"
                     /locus_tag="ACHKIR_000792"
     CDS             complement(74346..75992)
                     /gene="mqo"
                     /locus_tag="ACHKIR_000792"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - L-malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HFO9988578.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDAEKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLDLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
BASE COUNT        18508 a        20495 c        18920 g        18144 t
ORIGIN      
        1 gcgaggtaac atccctgtaa aacgcgccct cagcccacat ccatgtgggc tgccccggcc
       61 ttcagggaac gcctcggcga ttttgacgcc accaaacaac cgtgcggcct attgataaag
      121 agctaacaca ttgtcaaaaa acatcactat ggtttcttag agtttctcga tatcaattgc
      181 ctgaatagcc cttgcaatat caggggaatt attcaacacc cgaacatgct gaaataattc
      241 cgttgcttca tcgtattctt tacgcaaata actcaaccac tgtttaatcc gcgcaacgtg
      301 atataacccg gtatcgccct gcttttccag acgggtatat ttttgcagca aagcaactac
      361 ctccggccac ggcattcgcg gttcgttata ttttaccacc cggctcaggt tgggaatatt
      421 gagcgccccg cgaccaatca tcactgcgtc gcatccgctg atcgtcatgc attgttgcgc
      481 actctgccag tcccagattt caccgttggc aatcacagga atattcaacc gctggcgaat
      541 ctcgccaatc gcctgccagt caatatgctc cgcgcggtaa ccctgctctt ttgtccgccc
      601 atgtactacc agctccgtag cgccagcctg ttgcaccgca tcggcgattt caaatttctt
      661 ctcaccgctg tcccagccca gacgcacttt tacgctgaca ggcaaatgcg ccggtacagc
      721 ttcgcgcatc gcttttgcac cctgatagat gagttcagga tctttgagta acgtcgcccc
      781 accgccgcta ccgttaaccg ttttcgacgg gcagccgcaa ttgagatcca cgccccagga
      841 acctaactcg accgcacggg cggcattctc tgccagccat tgtgggaact gacctaacaa
      901 ctgcacgcgc accagcgtac cggctggtgt ccggctggcg ttttgtagct cagggcaaat
      961 gcgatgaaag acttttaccg gcagcagttg atccaccacg cggacaaact cggtgatgca
     1021 cagatcgtag tcgttaactt cggtcagcaa ttcacgcacc agagagtcga gtacaccctc
     1081 catcggtgcc agtaacacac gcatatcatc acccgcaaaa aaatgaggcg ctatgttagc
     1141 gcctatggtc agcggattaa agtactcagc gtggttgcaa acagttatct tctttccagc
     1201 cgtacagcca ctccatgccg cggtaaaact ctgcctgcga tttccctttc cacaacaggt
     1261 tacgtacctg gcgctctact cctgcggcat gataaacccg ccccatttca cgggtcgacc
     1321 agacgatgcg cgcggtgcgc ggaatacgta ccgactcata cagcgcaaag gcttgtgccg
     1381 catcaccctc acactgcgcc agcgccttac ccagtgttac cgcgtcttcc agcgccatac
     1441 aggcaccctg cgccatatac tgcgccaccg gatgggccgc atcgcccacc agcgtaatgc
     1501 gcttcgttcc ccacttcgct accggttcac ggtcggcggt tgaccagcgt cgccaggagg
     1561 tcggtttatc cagcatctgg cgcggacggg ggtgaatccc ttcaaagtaa gagagcactt
     1621 cctctttact gccatccttc acgccccact cttcctgctg gcggctgtgg aatgtcacca
     1681 ccaggttata ctgcttgccg ccgcgtaaag ggtaatgcac caagtgacag cacggccccg
     1741 cccacagtac tggtgcgtta atgcgcaaat cttccggcat atcggcacaa tcgataacag
     1801 cacggtagac cacatgcccg gtgacacgcg gcgcatcgcc caataagctc tggcgcacca
     1861 ctgacttcac gccgtcacag ccaatcagaa tatcgccgat ccaactattg ccgcgatcgt
     1921 cgaaaacggt gacatcgtct tctgtctcgc gaatatccac gacgttggtg gaggtgtgat
     1981 attccacgtc cggatgcgtc agtaccgctt cccacaccga ggcgtgaata tcaacccggt
     2041 ggatcaccgc gtaagggccg ccgaaatgat cgcgaaaggc ctgaccggtt tcaatgcgca
     2101 ccacttcttc ggcgtttacc gcatccatca tggtgatgtg atcggtgaat accgcgcgct
     2161 ggcgggcgat ttcgccgacg ccgaggctat ccaacgccga aaacgcattt ggccccaact
     2221 ggatacctgc gccaatctca ccgatctcat gcgccttttc cagcagcacc actttgatcc
     2281 cctggcgggc cagtgacaat gcagttgccg caccgccgat cccgcctccc acaattactg
     2341 cgcgtgtcac tttagtcatt atggttctcc tggtcaggct gaaattttat cttgttgatt
     2401 ttctggcgct gcggcaatga acgctggcag ctgcgtgcag gcgtcgtata ccgctttgca
     2461 gcgcggatac ccgctcagat cgcagttcat acgtagcgcg ttcgcccact gtggaacaag
     2521 gcagcaatct gccagcgtcg gcgtctcgcc cacacaaaat tgccccgact ggctctggcg
     2581 cagtagctgt tccaccgcgc ttaacccttg ctggatccag tgcgcatacc agcgtttttt
     2641 atcttcttca ctgacgttca gctcttcggt caggtagcgc agcacgcgta ggttattcaa
     2701 tggatgaata tcgcaggcga tggcatagac aatttccagc accttgttgc gggccggttc
     2761 ttcctgcggc atcagcggaa cttgcgggta gtgactctcc agccagtcaa taatcgccag
     2821 cgattgtccg agtgattcgc cctcgtcggt cagcagcgtc ggcaccagcc cgaccggatt
     2881 catccgccgg taggccagtt cgttttgctg accaatgcgg atattcaccc cttcggtctg
     2941 gtaatcgata cccttcagcg ccagcgcaat gcgcacccgg taagacgctg aactattaaa
     3001 aaaactgtac agcttcatca tcacctcaga caatcttcac cgcgataggc gttaagccct
     3061 cgacgttgcc cgtgatcaca tcacctttga ctactgagcc aaccccttcc ggcgtaccgg
     3121 taaagattag atcgcccggt tgcagttcaa agaagcctga caggtagctg atggtttcat
     3181 tcactgacca gatgagatga cgaatatcgc tgcgctgatg atcttcgccg ttaacctgta
     3241 accagatagg tgcgttatca ataccgccag tttcgctggc tttgtgcagc ggcgcgatgg
     3301 gggccgagag atcgaacgct ttgccgattt cccacgggcg gcccatctgg cgcatttcca
     3361 tttgacggtc acggcgagtc atatcaagac ccgtggcata accccagaca tattcatggg
     3421 cgttttccag cgggatatca ctgccttttt taccaatcgc caccaccagt tcaatttcat
     3481 aatggtagtt atcggtctgc gccggatacg gcagcgccag cgtttcgcca gcggcaaccg
     3541 gaacaatggc atccgccggt ttgcagaaga agaatggcgg ttcacgatcc ggatcaaagc
     3601 ccatttcgcg ggcgtgggcg gcatagttac gtccgacgca gtaaacgcgg cgtacgggga
     3661 attgttcatc actgccaaca accggtacgg taacgggcgc ttgtggttcg aatacgtatt
     3721 gagtcatttt ctctctccca aattaataac gtgcttcgcg gaacagcccc agggcttctt
     3781 gtaccggtct gtccgaaaaa ctaaataaaa cggtatcttg cgtggtctgg aacgacacgc
     3841 cgtgccaggt cggcaccacg aagatatctt ttgcactaaa gctgaaggtt tcattgccga
     3901 tagtgacctg cccgctacct tccaccacat ggtagatggt gctgtcagtg gtacgcgcca
     3961 cgcgtgaggc aaaccctttc ggcagcagtt gcagaaaagc gcccatcgac ggcatcggat
     4021 agccaccggt gaccgggttg acgtagcgca ttttgtagcc atcccactca tcggcatcgc
     4081 ccaggcgggt taaatcgtgt agcacttcgc ggctacggtc ataacggtag ttaaagatgg
     4141 gcgaggagtt cccggtctga tggcgcaacg gcaacatatt ggcagcgtaa cgcggcaggt
     4201 aatcgccctc tttacgcgtt actggttgtt gctcctccgg gtaatcttcg gcaaaaccgc
     4261 agcccagagt gttcactaat ggcagatcca gcccatcgag ccagataacc ggttcgtcgc
     4321 cagggttacc gtgatcgtgc cagcgccact gcggggtcag gataaaatcg ccctcattca
     4381 ttgacgtgcg ttcgccatca acggcggtga atgccccttt accttcaaca ataaaacgca
     4441 gcgccgactg gttatggcga tgactcggtg ccacttcgcc cggcatgatc agttgcaaac
     4501 cggcatataa agtcgcggtg atggaagact gcccgcgcag cgccgggttt tccagcacca
     4561 gcacgcggcg cacggcttct ttcgcgccaa tcaggctgcc gctctccagc agcaacgggc
     4621 ggatctcctg ataattccag tacgctggcg cacagttagc gttgggcgtt tttggcacca
     4681 gatggtgcag tgactcccac agcggggtca ggttttgccc ggaaatatgc tgataaaatt
     4741 gctcgcggct attttgattg ttatcagtca ttgggatgct ccttattcgt tcacaacacc
     4801 tgcgtgaggc agtgagtcag ggacagaggt cgactggcga accacggtga tcagctgggt
     4861 taacatcacc gcactgatcg ccgcgggcac agcgataatg aaaaacaggg tgtcgaaaga
     4921 gaaattcatc gccatcataa cgccgccaga cagcgagcca acaatcgcgc cgcagcggcc
     4981 cacggcgttt gaccagctca cgcccgtggc gcgactttgt gttggataaa gtgttgcggt
     5041 cagcgcattc agccccacct gcgatccgct gatgccaatg ccggtgccga aaatcgccag
     5101 cgccattagc cataatccgt cctggcttaa gccaatcatc acgatgcata cagcgcctat
     5161 ggcatagctc agcgccagta cgcggaaagg attgaactta tccataagca cgcccagcgc
     5221 cagcgcgccc aacgtaccgc caatctggaa cgcggcggta acccaggagg catgttgcag
     5281 gtcgatgccg cgatggttga gcagcgttgg catccagctt gagagcaggt aaataatcag
     5341 caagctcata aagaacacca cccacagcat caacgtgatg ggtaactgac gccccataaa
     5401 cagttgacga atgctgcctt ttgtaacgga ggctgattca atgagataaa agtgggtatc
     5461 gacataacgc tcgcggatga tggcgctgac ggttttcgca atcaccgcct ggggtagctg
     5521 gcggcgcacc tgccagcgtg gcgattccgg cagaaccacc agcagtgcaa caaacagcat
     5581 cagcggtaat acgccaccca acaccaggat cccgtgccag ccaatcaccg gcaccagctg
     5641 cgcgctgaca atcccgccaa acgccgaacc cagggtaaag ccgcaaaaca tcagcgtcac
     5701 cagcgcgccg cggcggcggg caggcagata ttccgaggtc atggtaattg tatttggcat
     5761 cgcgccaccc agcccaaggc cggtgaggaa acgcaggaat accagcgttt gcagatccgg
     5821 tgagaaagcc gaggcgaggc ttaacgcgcc aaacaaaaag acgcacagct caatcacccg
     5881 tttgcggcca aagcgatcgg acaacggacc acaaagcagt gcgcctgcgg ttaaccccag
     5941 cagacccgcg ccaaaaagcg gggcgagatc gcctgcggtg agttgccagt gagtgcgaat
     6001 atcaggggcg ataaagccga tagccgcggt gtcaaaacca tcgagcatta ccaccagaaa
     6061 acagcagatg ataacgcgcc actgcatctt gccgaccgga gcagcatcga tcagtgcttg
     6121 taaatcacgt cgttgagtca tagtaaatgc ctcagtgcag gtaaggtaga tgtttcattt
     6181 gttttatgta tgttgcgata ttgtgattaa agagatgaaa tgtacaatgg catttcggac
     6241 agagagataa cctggaggtt atggcaaatg gcaaactggg cgcagaaact aaagcttcat
     6301 cacctgcaaa tgctggtggc gctgggtgaa cagggcaatc tgacccatgt cgcgcggatg
     6361 atgaatatca cccagcctgc gctgtcgaaa tggttgtcac aactcgaaga tgagatcggc
     6421 ctcacactct ttgagcggca cagcaaagga cttcgtccta ctgaaggcgg caaattgtta
     6481 ttgcaacatg cccaacggtt aatcaatgac atggagcgct cgcaacatga actggcccgt
     6541 tttaaacaag gtggactggt cggcagtctg aaaattggct gttctcccgt cgccactgac
     6601 tgcgtatccc aggcgatttt gcatttgctc aaggagatgc caactctaca cctgaatatc
     6661 gaagagaagg tgatgacccc attgctgcaa gatctgttgg caggttctgt cgatgtggtg
     6721 gttgggcgaa ttggcggtcg cgcgttgcag ctgccgctga attatcgcgt gctctacacc
     6781 gagccggtgt gttttgtcgc ccgtcctgag catccactgg cgaaatacgc caccctcagc
     6841 tggaatgatc ttgctaactg gcggtggata gtctggccga caggtacccc catccgcatc
     6901 agcattgata atgcgctggt agaccacggc gtgatgctgc cagaaaacac gattgagtcg
     6961 gcgtcgatga acgtcagtac caacctgtta caaagcagcg atatgatctc tattttgtca
     7021 ttacgcctgg cgcaacgtta tgccagccag ggacaactgg tgattctcaa cctgccaaag
     7081 atagaacaga aaggcagcgt cggtgtgttc tggcgcaata acgaaacgcc ttcttatgct
     7141 ttaagtcagt ttctgcactt tttacagcag aattagtgtg ctgaactggg cgctcgcccg
     7201 aggttacgtc cctaaaattc aactatgctg agatttctta atgtgatcgg tagcacgttt
     7261 tacgaattaa ttgtatgatg aatccatctc atctggggtg ttgattatga gtaagacact
     7321 gaacattatc tggcaatatt tacgtgcttt tgtcctgatt tatgcctgcc tgtatgcagg
     7381 cattttcatt gcttccctgc taccggtaac cattccgggc agcatcatcg ggatgctgat
     7441 cctgtttgtc ctgctggcct tgcaaattct tccggcgaaa tgggtcaatc cggggtgcta
     7501 cgtactgatt cgctatatgg cgctattgtt tgtgccgatt ggtgtaggcg tcatgcaata
     7561 ttttgatttg ctccgcgcac agtttggtcc ggtagtggtt tcctgtgcag tcagtacgct
     7621 ggtggttttt ctggtggtga gctggagttc acaactggtg cacggtgaac gtaaagtcgt
     7681 aggtcagaaa ggatcagaag aatgatggcg aatatctggt ggtcattacc gctgacgttg
     7741 attgtctttt ttgccgcccg caaactggcg gcacggtata agtttcccct gcttaacccg
     7801 ttgctggtag caatggtggt gatcattcct tttttaatgc tgaccggcat ctcttacgac
     7861 agctacttta aaggtagcga agtgctaaac gatctgcttc aaccggcggt tgtcgcactg
     7921 gcctatcctt tatatgagca gctgcaccag atccgcgcgc gctggaaatc gatcatcacc
     7981 atctgtttta tcggcagcgt ggttgcgatg gtgacgggga cttctgtggc attgttgatg
     8041 ggcgcttcac cggaaatcgc cgcgtcaatc ctgccgaaat cagtcaccac gcctattgca
     8101 atggcggttg gcggcagtat tggcggtatt ccggcaatca gcgcagtttg cgtgattttc
     8161 gtcggcatcc tcggcgctgt gtttggccat acattgctta atgcgatgcg tattcgtacc
     8221 aaagctgcgc gcggtctggc aatgggcaca gcctcgcacg ccctcggtac ggcgcgctgc
     8281 gccgagctgg attatcagga aggtgcattt agttcgctgg cgctggtgtt atgcgggatt
     8341 attacttcgc tgatcgcacc gttccttttc ccgattattc tggcagtaat gggctaaaat
     8401 ttgcgatgcg tcgcgcattt ttgatgtaag tttcacgcgt tgcataatta atgagattca
     8461 gatcacatat aaagccataa cgggttcgta aactgttatc ccattacatg attatgaggc
     8521 aacgccatgc atccacgttt tcaaaccgct tttgcccaac ttgcggataa cttgcaatct
     8581 gcactggaac ctattctggc agacaagtac ttccccgctt tgttgaccgg ggagcaagtc
     8641 tcatcgctga agagcgcaac ggggctggac gaagacgcgc tggcattcgc actacttccg
     8701 ctggcggcag cctgtgcgcg tacgccattg tcgaatttta atgttggcgc aattgcgcgc
     8761 ggtgtgagcg gaacctggta ttttggtgcc aatatggaat ttattggtgc gacaatgcag
     8821 caaaccgttc atgccgaaca aagcgcgatc agccacgcct ggttgagtgg tgaaaaagcg
     8881 cttgtagcca tcaccgttaa ctatacgcct tgtggtcact gccgtcagtt tatgaatgaa
     8941 ctgaacagcg gtctggatct gcgtattcat ctgccgggcc gcgaggcaca cgcgctgcgt
     9001 gactatctgc cggatgcctt tgggccgaaa gatctggaga ttaaaacgct gctgatggac
     9061 gagcaagatc acggctatgc gctgacgggt gatgcgcttt ctcaggcagc gattgcggcg
     9121 gcaaaccgtt cgcacatgcc ttacagtaag tcgccaagcg gtgtcgcgct ggaatgtaaa
     9181 gacggtcgta ttttcagcgg cagctacgct gaaaacgccg cattcaaccc gactctgcca
     9241 ccgttgcagg gggcgttaat tctgttgaat ctcaagggtt atgattaccc ggatatccag
     9301 cgcgcggttc tggcagaaaa agccgatgcg ccgttgattc agtgggatgc cacctccgca
     9361 acactgaaag ctctcggctg tcacaatatc gaccgcgtgc ttctcgctta agcctggtgc
     9421 cggatgcggc gtgaacgcct tgtccggctt gccagccctc tcctggtgtc gaaaatcccg
     9481 gcaaacagtt tgccgtttct tgcgcaaaac cagcgggtaa agtagcctga tggaaatttt
     9541 ccttagatcg agtctcctgc atgttaaagc gcgtgttcct cagcctgtta gtcctgatcg
     9601 gcttgctgct gttgactgtg ctcggcctcg atcgctggat gagctggaaa accgcgcctt
     9661 atatctacga cgaattgcag gatctcccct accgccaggt cggtgtggtg ctcggaacag
     9721 caaaatatta tcgtactggc gtaattaatc agtattatcg ctaccgcatt caaggagcga
     9781 ttaatgccta taacagcggt aaggtaaatt atctattact gagcggcgat aacgcattgc
     9841 aaagttataa tgagccgatg accatgcgca aagatttaat cgctgctggt gtcgacccat
     9901 cagatattgt tctcgattac gcaggctttc gtacgctgga ttccatcgtg cgtacacgca
     9961 aagttttcga tactaatgat ttcattatta tcacccaacg tttccactgt gagcgggcat
    10021 tatttattgc gctgcatatg gggattcagg ctcagtgtta tgccgtaccg tcaccgaaag
    10081 atatgctgtc agtacgtatt cgcgaatttg ccgcccgttt cggtgcgctg gctgaccttt
    10141 atatttttaa acgtgaaccg cgttttttag ggccgctggt ccctattccg gctatgcacc
    10201 aggtgcctga agatgcgcag gggtatcccg ccgtcacacc cgaacagtta cttgaattac
    10261 aaaagaaaca aggaaagtag ttatggatgt acagcagttt tttgtcgttg ccgttttttt
    10321 ccttatcccg gtattttgtt tccgcgaagc atggaaaggc tggcgcgcag gcgcgattga
    10381 taaacgggtt aaaaatgcac cggaaccggt gtatgtctgg cgagcaaaaa atcccggact
    10441 cttcttcgct tatatggtgg catatatcgg cttcggaatt ttatctatcg gcatgattgt
    10501 ttatcttatt ttctatcgtt aatattttct cttttctgtt aattattgag aataattatt
    10561 acttcactca ataagctgcg gatataacat tctgaaccgc agctatttgt gaatcctttc
    10621 acagtttaaa tttccccacg cgcatagcct taatatcagc acgcaatatt tatactctaa
    10681 ataattcgag ttgcaggaag gcgacaagtg agtgagtccc caggagctta cataagtaag
    10741 tgactggggt gagcgaacgc agacgcagca catgcaactt gaagtatgac gagtatattt
    10801 agcgttcgcc ttaattactc atagcattaa ggaagatcac atgccgcaac aaaattatct
    10861 ggatgaactc actccggctt ttacaccgtt actggcgatt aaagaagcct ctcgctgttt
    10921 attatgccat gacgccccct gtagtcaggc ttgcccggca cagaccgatc cggggaaatt
    10981 tattcgctca atctactttc gtaattttaa aggcgctgcc gagacaattc gtgaaaataa
    11041 tgccctcggt gccgtttgtg ccagagtgtg cccgacggag aaattatgcc aaagcggttg
    11101 tacccgtgcc ggtgttgata ctcccattga tatcggtcgc ttacagcgtt ttgttactga
    11161 ttttgaacaa caaactggga tggaaattta tcagcccggt actaaaacgc tcggcaaagt
    11221 cgccattatt ggcgcgggtc ctgccggatt gcaggccagt gtgacactga caaaccaggg
    11281 ttatgacgtc acgatctatg agaaagaagc acaacccggt ggctggttgc gtaacggcat
    11341 tccgcaattc cggttaccgc aatcagtact ggatgcagaa atcgcccgta ttgaaaaaat
    11401 gggcgtgacc attaaatgca ataacgaaat cggtaacaca ctcaccctgg agcagctgaa
    11461 agcggaaaac cgcgcggtac tggtcactgt ggggttatcg agcggttcgg ggctaccact
    11521 gtttgagcat agcgatgttg agattgccgt cgacttcttg caacgtgcac gacaggcgca
    11581 aggcgatatc agcattccac aaagcgcatt aattatcggc ggcggtgatg tcgcgatgga
    11641 cgtagccagc acgctgaaag ttctcggctg tcaggcagta acttgcgtag cgcgtgaaga
    11701 gttagatgag tttccggcaa gcgaaaaaga gtttgccagc gcccgggagc tgggcgtttc
    11761 gatcatcgat ggattcacgc cagtagctgt ggaaggcaat aaagtcacgt ttaagcatgt
    11821 acggttaccg ggcgaactga cgatggcggc agataaaatt attctcgccg tcggtcagca
    11881 tgccagactg gatgcctttg cggagttaga gccgcagcgt aacaccatca aaacacaaaa
    11941 ttaccagacc cgcgacccgc aagtctttgc tgctggcgat attgttgagg gtgacaaaac
    12001 cgtggtttat gccgtgaaaa ccgggaaaga agccgctgag gcgattcatc actatttaaa
    12061 gggagcttgc tcatgttaac gaaagatctt tcgattacct tttgtggtgt gaagtttccc
    12121 aacccgttct gcctctcttc ttcgcctgta ggcaactgct atgagatgtg tgccaaagcc
    12181 tacgacacag gctggggtgg cgtggtgttt aaaacgatcg gtttttttat cgccaacgaa
    12241 gtctcgccgc gttttgatca tctggtgaaa gaagataccg gttttatcgg cttcaaaaat
    12301 atggagcaga ttgctgaaca tccgttggaa gataatctgg ccgcactgcg tcggctgaaa
    12361 gaagattacc cggacaaagt attgatcgct tcaatcatgg gcgaaaatga gcagcaatgg
    12421 gaagagctgg cgcgtctggt gcaagaagct ggcgcggata tgatcgagtg taacttctcc
    12481 tgtccgcaaa tgacttctca tgcgatgggt agcgatgtcg ggcaaagccc ggagctggta
    12541 gaaaaatatt gtcgggcagt aaaacgtggt tccacgctgc caatgctggc gaagatgacg
    12601 ccgaatatcg gtgatatgtg cgaagtggcg ctggcggcga agcgcggcgg cgcagatggc
    12661 attgcggcga ttaacaccgt taaatccatc accaatatcg atcttaatca gaaaatcggt
    12721 atgccgatcg ttaacggtaa atcgagtatt tccggatatt ccggtaaagc ggtaaaaccg
    12781 atcgccctgc gcttcattca gcaaatgcgt acccatccag aactgcgcga tttcccgatc
    12841 agcggtatcg gcggcattga aacctgggaa gatgcggcgg agtttttatt gctcggtgca
    12901 gcgacgttgc aggtgaccac cggcattatg cagtacgggt atcggatagt ggaagatatg
    12961 gcaagcgggt tgtcgcatta tctcgccgat caagggtttg attcgttgca ggagatggta
    13021 ggtctggcga ataacaatat tgtcccggcg gaagatttag accgcagtta tattgtctat
    13081 ccccatatca atctcgataa atgtgttggc tgtggacgct gttatatttc ctgttacgac
    13141 ggcggtcacc aggcgatgga atggagcgag aaaacccgta caccgcattg taataccgag
    13201 aaatgtgttg gttgcctgct ttgtggtcac gtctgcccgg tgggttgtat tgatctcgga
    13261 gaagtgaagt ttaagaaggg agagaaagaa cacccggtaa cgttgtaaaa ccagagacgc
    13321 atccggcatt tggcaccgat tgccggatgc ggcgcatccg gcaataccca cattcacatc
    13381 atttcttacg cgcgtatttc agtgaatcca gcgccacggc gaagataata atcgcccctt
    13441 tgatgatgta ctgccagtat gggtttacgc cgatgtacgt caggccatag ttgatgacgg
    13501 taaaaataat caccccggtc accacgccaa tcaccgtccc tacaccaccg ctgaacgata
    13561 caccacccac cacacatgcc gcgatagcat ccaactcata cataaagccg aggttgttgg
    13621 tggcagagcc gatacgtccg gcttctaaca tcccgccgaa ggcatagaac acgccagaca
    13681 acgcgtagat catcagcagg ttcaggccga cgttgacacc agatactttc gccgcttccg
    13741 ggttaccgcc aatggcaaaa atgttcttac cgaagcgggt tttgttccac aacacccaga
    13801 cgaacgccac cgcaatcaac gcgtagaagg tgatgtaaga gagacggaaa ctccccagcg
    13861 cgacaaagcc ctgagcaaag gtagagaagc cactgtcgaa accagaaatc ggcgacgccc
    13921 cgacaaagtc gtaatagagc gagttgatgc catagacgat gatcatcgtg ccgagcgtgg
    13981 taatgaacgg cgtcacgttg agataggcga taatcagacc gttgatcaaa ccgatcaccg
    14041 caccaatggc acagacaatc agaataacca gcgcaatcgg catcgtcgcc atttccggga
    14101 acactttgtt ggcgttatcc atggactgca ataacgtcgc cgctactact gccgccagcc
    14161 ccacctggcg acctgcagaa aggtcggtcc cctgggtgac aattaaccct gccacaccga
    14221 gcgcgataat aatacgcacc gatgactggg tgagaatatt acttaagttc aacagactta
    14281 aaaatgttgg gtcctggaaa ataataattg ccagcaaaac taaaagaacg acgtaaatac
    14341 cgccctcttt caggtaagta agaaaacttt tcttatttaa cgcactcatg ggaagcccct
    14401 aatcttaaag gtgcaaagac gcaagacgca gaatttcgtt ttgcgttgtt gtttttgtat
    14461 cgacaattcc ggaaacgaga ccattgctca tgaccagaat acggtctgtt atccctaaca
    14521 actcaggcat ttcagaggag ataataataa tccctttgcc tttcttcgcc agttcggcaa
    14581 ttaactgata gatctcaaac ttcgctccga catcaatacc gcgcgtcggt tcatcgagca
    14641 ttaatatttc tggttgcgtt agtagccagc gaccaataat caccttttgc tgattaccgc
    14701 cggagagcga accaatttgc gtccgatgcc ccggtgtttt tacccgcatc gaatcaatca
    14761 cccactgggt atcgcttttc atccgcgagt tatccagtaa accaacttta tttttgtagt
    14821 tgcgaatatt ggaaattaac gagttaaaac cgatatccag ataggcataa attcccgttg
    14881 agcggcgctc ttcagttacc agtgcaaatc cgtggtttat ggcttcgttg gcattatggt
    14941 tattgatctt tttgccgtgc aacgtaatgg tgccagccga tttctcgcga ataccaaata
    15001 acgtctcaac aatatcggta cgtttcgctc ccaccagacc ggctataccg agaatctccc
    15061 ctttatgcag atcaaacgag acatcgcgaa tcgacggctg gcgcagcgac gtcaggttac
    15121 gcacttcaag aatcacttcg cccggcttgt tttctttgtc agggaaacgc tggttaagag
    15181 aacgcccaac catcatggcg atgatcttgt ccatcgtcag tcctgccagc ggttcagtgg
    15241 cgatccactg accgtcgcgc aatacggtaa cttcatcaca taactggaag atttcttcca
    15301 ttttatgtga gatataaaca ataccgcagc cgcgctcttt taatttacga ataatagtga
    15361 acagatgatt gacctctttt tcggttaacg aagaagtcgg ttcatccata atcacaattt
    15421 tcgcgttata ggaaaacgct ttggcgattt cgatcatctg catttgcgaa acggataatg
    15481 tgccgacgcg cgcacgcgga tcgatatcaa tatccagttc atcaaaaatc gctttggttt
    15541 cgcggtacat tttgtcctga tcgacaaaca tgcctttggt gggatatcgc cccagccaca
    15601 tgttgtccat caccgaacgt tgtaatacca ggtttaactc ctggtgtacc atcgaaatac
    15661 cattttccag cgcttctttg gcagaatgga aatcgatctc tttaccctgg aataaaatgg
    15721 tgccggagtc tttttgataa ataccaaaca ggcattttaa taatgttgat tttcctgcgc
    15781 cgttttcccc cattaatgca tggatagaat gtggccggac ttttaaatta acgttatcaa
    15841 gtgccttaac acctggaaaa gacttgttga taccgctcat ttccaacaag tattccccgg
    15901 aagacggagt cgttgagctg accatataat tttaccttgt tggccataca ataagggcgc
    15961 agtaaacgac tgcgcccaat cagtcttatt tcttgctgaa ttcagccagg ttgtctttat
    16021 ctacgccaac ataaggtacg cggaccactt tgttgtcgat tttccagttg gtgccatcag
    16081 ccgcaccttt gccatcggcc aggtttttcg ccagatcaaa ggtcgctttc gcctggttgt
    16141 tagcatcgtt cagtacggtg cccgccagtg caccggattt caccagcgcc agcgcttctg
    16201 gcagcgcatc gacgccaaac accggaatgc tggacttgtt gtgtgctttc agtgcttcta
    16261 ccgcgcccat tgccatcgca tcgttgttgg cgataaccac ttcgattttg ttggcgttcg
    16321 ggccagacag ccaggcgtcc atcttatctt tcgcctgagc ggtatcccac atagcggtat
    16381 ctaactgtaa ctgttcagtt ttgatgcctt tgtcgttcaa ttctttaatc acgtaagtgg
    16441 tacgtgcttc tgcatccgga tggcccggct cacctttcag cagtacgaac tgaatctgac
    16501 cgtctttgtt cagatcccaa ccctgattcg ccgcccagtg tttagcaatc aaatcgccct
    16561 gaataatgcc ggactctttg gagtcagtgc caacgtagta ggctttgtcg tagctatcca
    16621 gcgccttacg agacggttct ttgttgaaga aaaccaccgg cacgttttgc ccacgcgctt
    16681 tctcaatcac cgtacccgca gctgccgggt caaccaggtt gattgccagt gccttcaccc
    16741 ctttcgccag caatacgtcg atctgatcgt tctgcttgga ctggtcattc tgagaatcat
    16801 tcatcagcag ctgaacatct ggcgcggctt tcgcatcttg ctcaatagcc ttgcgcacca
    16861 cagacataaa gttatcgtcg tacttataga ttgttacacc aatgcgagta tcagcagcgt
    16921 gtgcagcggc accgaataac atgctggcca tcacagcaaa cagggttaac accttcttat
    16981 tcatggtatc tccggttttt cttatgcagg gtagtgcttg agataaatgc tcggcggggc
    17041 agtagagtta atgaagtgtt actgaacgcc gaagctcact ttttaaaatt cgttcttcca
    17101 tgcccggtaa cgctccagaa aacggcttta attgttgtta tgacgctatt acctcggtga
    17161 aagtgattat tcactgttac atacgggtta caacgttaaa acggtgcaat catagctatc
    17221 acattgttaa gatactgtga aatcactcac agattgaaag cggttacatc gccagatttg
    17281 ttgagttagt gatcgccgcc gcattctggc gcgttgcgac agaatggcga cgcactaacg
    17341 tcggcataaa acagtggctg gcacgcggat caatattgcc tgctgccccc tgcaaggcca
    17401 gttcggtggc taatttagcc attgaagcaa tgggataacg cacagtcgtt aattgcgggt
    17461 cggtgtaacg ggcgatggga atatcatcga aaccgatgat ggagagatgt aacggaatcg
    17521 caatgccatt atcttttaat gctgtcagtg cgccagcggc catattgtcg ttataagcaa
    17581 atacagcggt aagttgtaga ttgcgcccca gcagttcaac catcgccgcc tcaccgcccg
    17641 gcatgtccgg cgtacctgtg ccaatccagc tttccggcgg aataatatcc tgctctttca
    17701 acgcactcat ccagcctgct ttacgcatgg cgtcatcttc aatgccgtga ctggaagaaa
    17761 gataaccaat acgttgatga ccgttattca gcaacatgcg cgtcgccatt cgggcaccgc
    17821 tgagattatc caggcaaacg caacgatggg cgtaccccgg cacaacgcgg ttgattaaca
    17881 ccataccagg aatgttatcc ataaattgtg ccagttcatc atcactcaat gcttttgagt
    17941 gaacaatcaa cgcattacac cgctggcgaa ttaacacctc aatggcgtga cgctcttttt
    18001 ccgcttcatg atagctattg cctattagca cgtatttctg atgctgctgg gcgaccaaat
    18061 ccaccgcttt taccagcgca ccaaaaaacg catcagaaac gtccatcacc accacgccaa
    18121 tggtatcgct aacctgagtt gccagcgcct gggcattggc gtttggccga taatccagct
    18181 cactcacggc tttcattact gcttcacgcg tgtcggcact gaccagcgtg ctgttattga
    18241 gcacccggga aaccgttgcc acagagacgc cagcctgacg cgctacatca cgaatggtga
    18301 tcatattcac tacctgttat gagattgcag taaatgactg cttgctggcg gctattttgt
    18361 cagcactgaa tacaggactt cgtgaatcga gtcacagcaa tggaaacggt tacagccgtt
    18421 ttgttaacga atgtgatcca gatcgttatc ttgatgtttt agatcttgcc ggcccagctg
    18481 cacgcaaagt taactgacgc cagagccatt ccaccggccc ctggcggaag taacgcagcc
    18541 agataacaga gaagaggata ttcgccagcc ataccggaat aacaaacgcc agcagctcca
    18601 ggcggtcaaa atgcataaac aaaccgaggt ggtaaaaaag cgtggtacaa atcagcgttt
    18661 gcaatagata gttggtcagc gccatccgtc cgacgcaggc gatcgcaagc accagcttaa
    18721 agcggctcaa ttgcggccag aagccataaa acagcgacgc atagccaatc gcctgaaacg
    18781 gcgcactcag ttcccgcggc atctgaagta aaaaggcaca ccagcgatac gcccagtcca
    18841 gctgccattg cagggcgatg gcaggaaggt taatggtcac cccaatcgcc accagcacaa
    18901 aaccagtacg acgatagtga cgtaagctga actgcccttt cagccagccg ctgcgcatca
    18961 gcgccgcacc aatgagcatc atccctgcca gttgccagcc atactgtgcg cccagtgcca
    19021 gtaaactgtt gccaacacca tcggcacgat tactgatcgc ttcaacacca ccgtgaagtt
    19081 tccagtactt ttcatacaaa atagccgatg catcaggtgt ccaggcgcgg ctggtctggc
    19141 tatcggaaat caaccccaat aacagcaaaa cgccaagccc caccagataa agcatgacgc
    19201 cggtattaaa caggcttttt accgatggcg catcgcgcac cagccgccag cagattaagc
    19261 ccaccagccc gtaagccagc agaatatcgc cgtcccagaa caataaaccg tgaataaagc
    19321 ccagcaagac taacagcgtt aaccgcgact ggatccagcg tctgccacgg ggcagcaaca
    19381 tttgcaggcc agcaccaaac agcagcgcaa aaagcgtgag gaatttcacc tggccgatga
    19441 gatcgagaaa tgcccaggtc catgcatcct gcggcgtaat agcgccgtac caggcgggat
    19501 taagataagc cgcctttggt agcccaaagg cgctgatgtt taatagcagg atccccagaa
    19561 tggcgacgcc gcgaacaaaa tcgagcgtga cgttgcgccc catggttcct gccttttaat
    19621 cagttgtgat gacgcacagc gcgcagaaat tcgtggcgcg tattctgact ggatttgaac
    19681 aatccaccaa gagaggtcgt tgttgtggca ctggttgcgt cgcggatgcc acgcgctttc
    19741 acgcagtaat gcaccgcgtc gatagagaca gccacgttat tggtgcccag cagcgtttgt
    19801 agcgcaatga gaatttgctg ggtcagacgt tcctgtacct gtgggcgctg ggcaaagaac
    19861 tgcacgatgc ggttaatttt tgacagaccg atcaccgaat ctttcgggat ataagccacc
    19921 gtcgctttgc catcgatggt aacaaaatgg tgttcacagg tgctggtcag ggtgatatcg
    19981 cgcacggtga ccatttcatc gaccttcatt ttgttttcaa tgagggtgat tttcgggaaa
    20041 ttggcgtaat ccagaccgga gaaaatttca tcgacataca ttttagcgat gcgatgcggc
    20101 gtttccatca aactgtcatc agccaggtcg agattcagca gctgcatgat ttcggtcata
    20161 tgaccagcaa taaggctttt gcgcgtttcg ttatccattt catgcacggg cgggcgcagc
    20221 ggtgtttcca gtcctcgcgc aactaacgct tcatgaacca gggccgcttc tttactgagt
    20281 gatggcattt atgatttctc ctgcaggtgt gacgcctccg ccctgcgtgg gggcaaagtt
    20341 attaagctga tttacagcct gattattgtg cgtgaggcgg cgcacataat ccagtattca
    20401 cagcgataat tattgtaatt gccgctgcct ttcatcagca gatgttaaaa catcgttatg
    20461 caaatacgga agtgaaagtt actcacagca cattgaataa acggtatgat gaagaaattg
    20521 caaacaacac aacaaggagc cacgcatgga aatgctcgaa gagcaccgct gttttgaagg
    20581 ctggcagcaa cgctggcgac acgactccag taccttaaac tgtccgatga cgttcagtat
    20641 ctttctccca ccacctcgtg atcacacccc gccaccagtg ctgtactggc tttccggatt
    20701 aacctgcaat gacgagaact tcaccaccaa ggcgggtgcc cagcgcgtgg cggcagaact
    20761 ggggattgta ctggtgatgc cagacaccag cccgcgcggc gaacaggttg ccaacgacga
    20821 tggctacgat ttaggccagg gcgcaggctt ttatcttaat gccacgcaac cgccgtgggc
    20881 gacgcattac cggatgtatg attatctgcg cgatgaatta ccggcgctgg ttcagtcgca
    20941 atttaatgtc agcgaccgct gcgctattag cggtcattca atgggtggtc acggtgcgct
    21001 gattatggcg ctgaaaaacc cgggcaaata caccagcgtt tccgcctttg cgccgattgt
    21061 gaatccatgc agcgtaccct ggggcattaa agcgtttagc agctatttag gtgaggacaa
    21121 aaatgcatgg ctggaatggg acagttgtgc gctgatgtat gccagtaacg cgcaggatgc
    21181 gatcccgacg cttatcgatc agggtgataa tgatcagttt cttgccgacc agctgcaacc
    21241 cgcagtgctg gcagaagccg cccgccagaa agcgtggccg atgacgctgc gtattcagcc
    21301 gggatatgat cacagttact acttcatcgc ctcttttata gaggatcacc tgcgcttcca
    21361 tgcgcagtat ttactgaagt gaaagtccgc ccggttcgcc gggcatcttc tcatcagaag
    21421 cgataatcca ctgccataaa gtaacgacgt ccgtcttcgt tatagctgta gtcgtcacga
    21481 ctgagatcct tgtcgccaag gttcagcacg cctgcgcgca gtttgacgtc tttagtcacc
    21541 tgccaggccg caccggtatt ccagatggta taaccgcccg gtgttttagc cgtcgcgctg
    21601 tcggcgcgtt tctgcccggt atagtgtccg gaaacataga atgaccagtc ttccagcgcc
    21661 agcggtttcc agtccagcgt accgttagcg gtatggaacg gcagatcgga taaaggttta
    21721 ttttcgccgt tgctgacatc acgaccatcg ttgtaggtgt agttgagtga cagtttccat
    21781 tcatcgttga acggaatttt cagctcggtt tccacgccct gaatacgtgc tttgttaacg
    21841 ttgtagtagc taaataccgg cacaccgttc gctgtaaaac caacaaagtt ttggtagcct
    21901 ggtgcagcat tgacgtcaga cgtacggctg atgctgatac gatctttcac atcgttacgg
    21961 aaaacggtaa cgctggattc aaccccttcc agccagcctt cttcgcccat gtagtaaagc
    22021 cccagctccc aactttcgct ggtttctggt ttcagatccg ggctacccac aatcttacat
    22081 gcgccacggc aggaattgct cgtccagtca gggctaagtt gcaacagaga aggtgcttta
    22141 aatgccgtcg cccagccccc tttcaccgtt acggtgtcgg tggcgttata aaccaggtag
    22201 gcacgcggac tccagtgttc accgtaggtt tcatgatcgt ccatacgtac gccggtcgtc
    22261 agcgccagcg gctcgaagat ccgccattca tcttcaacaa atagcgcgta ctggctggca
    22321 gatgttttgg agctggttcc cccagtcagg ttcaccgcat cgctaagttt gtcgtgacgc
    22381 cattcaccgc caaccgtgag aaactgatta atcgccgtca gcggcaacgt gtatttgccg
    22441 tcgaccgtat tgctttcgga agttatcggg ctgctgttgc cagggttttt gttctcgact
    22501 ttctcaccgt agtatttcag ttcgctggtg ccgtaatccc aacgcccatt atggctgacg
    22561 gagtagttct ggcgttccag gcggtttttg tccagcgagt cggaatcacg atcctgacgg
    22621 tcgaaaccgt atccggcagt aaaatcgtga ttttgattcg gtgtccaggc aaattcgaca
    22681 ttgccgtcgc ggctggagaa tccttcaata cgcggcgttt ctccggtatc ggtggtcgtt
    22741 gagttttgcg ggtcatcctt ttcacgtttt gccaggctgc cgtaagcttt cattcccagc
    22801 acgccgtcaa ttaatggtcc gctggtaaag aattgaccgt tataggtatc accgcgatcg
    22861 cgatgttcct gaacggtggt atcgacggta acggtgcccg accatttctg accgattttt
    22921 ttggtgatga tattcactac accgccgagc gcatcggaac cgtacagcga cgacatcggg
    22981 ccacggacca cttcaatacg ttcgatggaa tcgaccggga tccagttcag atcgaaatca
    23041 ttgtggcgga agacggcatt gcgggagtta acgcgtttac cgtcgacgag aatcagggtg
    23101 tagctgctgt ccagaccacg aatactaacg cccttacggt tatccccttc gttcgtcagt
    23161 tgtacgccag gcacttcttt gaggacatcc ttcagattct gtaccggttt tcgctgcagg
    23221 tcttcctagg taatgacgct gatactggca ggtgcatctt taaggttttg ctcaacggaa
    23281 gatgcagtga caaccatcgt ttcgccatca tcatcgaccg ctaacacagg ccatgcacaa
    23341 gaaatagcgg acaaacacag cccgacccgt acgaaagggt tcaacctaaa cattccatat
    23401 ctccatgagg taactacgaa aataaaatgg gttatcgctc acatcttctt cacgtcccct
    23461 tgcgtacggc agcatcgcgg tggacttatt tgtatgcagg tgatcatccc agaaagccag
    23521 accagcctct gatttgtcag ccttttttga ttgcggcgta acgataatgc aaacgataac
    23581 aattatcaat ccaaatgtta aattttatat ccgcatgatt tgtcagggaa atggaattga
    23641 aaaagccctc tcggttgaga gggcttagca aggaagggag aaaacttatt tcttatcgtt
    23701 ctgcgggaac ttcatttcgc tgtagcgtac aaagtgagtt cctttaatca gcttgtagcc
    23761 gaaccaaata atcaggaaca gcgggatacc aatatacgtt gccgctacgc cgccccagtc
    23821 aatggtatct ttcaggaacg cttcatagtt ctggcccaaa gtgataatca gacacagaat
    23881 gaatgcgaag atcggcccca gtgggaagaa acctgaacgg tacggcagat cgttaatgtc
    23941 gtgtccctgc aatacgtaac cgcgacggaa gcgatagtgg ctaacggcaa tccccagcca
    24001 ggcgataaaa ccggtcatcc cggaggtgtt cagcagccac aggtataccg tctggttgcc
    24061 aaacatggag gtcaggaagc acagaccggc aatcaccgtc gtcgcataca gcgcattacg
    24121 cggcacgcca ccacgcgaca gtttagcgaa aatgcgcggc gctttaccgt cacacgccag
    24181 ggtgtacagc atacgagtag acgcatacat accggagtta cccgctgaca gcaccgccgt
    24241 cagaataact gcgttcatca ccgctgcagc agagagcaga cccgcgtgct ggaacaccag
    24301 ggtgaacgga ctgacgctga tgtctttaac atcgttacgc agcaggctcg gatcggtgta
    24361 cggaataatc aggctgataa tcaggatcgc gaacacatag aacaacagga ttcgccagaa
    24421 cacctgacgt accgcgcgtg gaatgttttt cgccggatct tcggactcgc ctgcagcaat
    24481 accgatcagc tcggttccct ggaaagagaa gccgacaatc atagctacgc cgatcatcgc
    24541 cgcaaaacca ccagcaaacg gcgcttcgcc gattgtccag ttgctccagc ccgcaggctg
    24601 cgcgcctttg aagataccga taatcatcag cacgccaacg atgataaaga caataactgt
    24661 cgtgactttg atcagtgaga accagtattc cgcttcacca aagccacgaa ctgagatgta
    24721 gttcagcagg aagataacgc cgaggaacag cgcactccag atccagcccg gtgtatccgg
    24781 gaaccaccag ctcatgacca gctgagctgc aaccaggtca acggcgatag tcaccgccca
    24841 gttgtaccag tagttccagc ccagcgcgaa gccaaagcct tcttcaacat agttctgacc
    24901 gtaagtggca aacgaaccgg aaaccggcat atatgcagcc agttcaccga gactggtcat
    24961 caggaagtaa accatcaggc caatcaacat atacgagagc aatgccccgc ccggacctgc
    25021 ctgagaaatc gttgcgccag aggcaacaaa aagacctgta ccgatggaac cgccaatggc
    25081 aatcatcgtc aggtgacgcg cctttaattc acggcgtaag cccggcgctt ctgtggtttt
    25141 agtttcggaa accatacaaa aatgctatcc atcttaaaaa tgaggcgcga ttgtagcaga
    25201 cgattggcaa tccttccggc agaaatacac ggttataaga caccttcatg atcgcccagg
    25261 gattataagt aaagcagcca atctcttttc tggcgaatgc atgctgaggg cgctgcgtca
    25321 cgaaatcgac acagcaccag catgttcttg tacagcaact taacgcggta cattcgcggg
    25381 atcgcaatag tccagaaagc gccgtagcgc gttggaaagg tgtttttgcc gatgatgtat
    25441 acgccacaac gtacgcatca ggcgcggcag agggaccgca acttcactta atgtgcctgc
    25501 ctgcaattga tcttcaatca cccgtcgcga caggcaacta attcccaacc catgacgcac
    25561 cgcatgtttg attgcctcgg agttacctaa ttccatcgcc atctcaaact tcggtaaatg
    25621 tgacagcaac agataatcga caatctcccg cgtgccggag ccgcgttcac gcaggatcca
    25681 cggcgcagcg gccagctgtt ctaaggtgac cggaccacga gccaatggcg aagtcggcgc
    25741 ggcgaaaacc accagctcgt cttccagcca cggttcagaa atgatttcag tgctgtggca
    25801 cggtccttca ataaagccaa tatcaacgcg gaaatccagc accgcttgca tcacgtcctg
    25861 gctgttccca acgctaagtt caatcggcaa ctgcggataa tcatggcgat aacgggcaat
    25921 gactgcaggc agaatgtagt taccgatagt actactggca tagatacgaa tcgcgccgtt
    25981 gtcttcgcga aacagttgtt cgatttcaac cgcctgttca agcagtgcca acgcgcgcgg
    26041 atagagcagc cgcccgtgtt cattaacaac cagtcttttc cccacgcgat caaacagttg
    26101 cacgccaagc tgcccttcca gatcggtcaa ggctgcgctc actgctgatt gcgacaacgc
    26161 cagcatcacc gacgcctggg tggttgatcc acttttcaat acttctgcaa aaacttccaa
    26221 ctgccggagg gtgatgtgca tagtcgctta ccacttataa agattaatta taaatatata
    26281 atcaatttta tttttaaacc agttagtcgt aaccttatgc ccggttaaag gagagggtta
    26341 tgacgaatat caccttacag aaacaacatc gtacactgtg gcattttatt ccggggttag
    26401 ccctgagtgc agttatcacc ggggtcgccc tgtggggtgg ttccattccc gcggttgcgg
    26461 gtgccgggtt tagtgccctc accctcgcaa tcttgttggg gatggtttta ggcaacacca
    26521 tctatccgca catctggaaa agctgtgacg gtggtgtgct gtttgccaag caatatttat
    26581 tacgtctggg tatcattctt tatggcttcc gtctgacgtt ctcgcaaatt gccgatgtcg
    26641 gtatcagtgg gatcatcatt gacgtgctga cgctgtccag taccttcctg ctggcttgct
    26701 tcctggggca gaaagtgttt ggtctggata agcacaccag ctggttgatc ggtgccggta
    26761 gcagtatctg tggtgctgcc gcggtactgg cgactgagcc ggtagtgaaa gcggaagcca
    26821 gtaaagtaac cgtggctgtt gcaaccgttg ttatcttcgg gaccgtcgcg attttcctct
    26881 acccggcgat atatccgctg atgtctcaat ggtttagtcc ggaaactttc ggtatctaca
    26941 ttggttctac tgtgcacgaa gtggcgcagg tggtggcggc aggtcatgcc atcagcccgg
    27001 atgcagaaaa cgcagcagtt atttccaaaa tgctgcgcgt gatgatgctg gctccgttcc
    27061 tcatcctgct ggcggcgcgt gttaaacagc tgtctggggc gaacagcggc gagaaaagca
    27121 aaatcactat tccgtggttt gccatcttgt tcatcgtagt tgccatcttt aactcgttcc
    27181 acctgttacc gcagagcgtg gtgaacatgc tggtaacgct ggataccttc ctgctggcaa
    27241 tggcgatggc ggcgctgggt ctgaccactc acgtcagcgc gctgaaaaaa gccggggcga
    27301 aacctctgct gatggcactg gtactgtttg cctggctgat tgttggtggt ggtgcgatta
    27361 actatgtgat tcaaagcgtc atcgcataaa ccactacatc ttgctcctgt taacccgcta
    27421 tcattaccgt tttcctccag cgggtttaac aggagtcctc gcatgaaata cattggagcg
    27481 cacgttagtg ctgctggcgg tctggcaaat gccgcaattc gcgccgccga aatcgacgca
    27541 accgcgtttg ccttgttcac caaaaaccaa cgtcagtggc gtgccgcacc gctcacgacg
    27601 caaaccatcg atgaattcaa agccgcctgt gaaaaatatc actacacatc ggcgcaaatt
    27661 cttccccacg acagttatct gattaacctc ggacatccgg tcgctgaagc tctggaaaaa
    27721 tcgcgcgatg cgtttataga tgaaatgcag cgttgcgaac agctggggct ttctttgctc
    27781 aacttccacc ctggcagcca tctgatgcag atttcagaag aggattgcct tgcgcgtatt
    27841 gccgaatcca tcaacattgc gctggataaa actcaaggtg tgacagcggt gattgaaaac
    27901 accgccggtc agggcagtaa cttagggttt aaattcgaac atctcgcggc gattatcgac
    27961 ggcgtggaag ataaatcccg cgtcggcgtc tgcattgata cctgccatgc ttttgctgcc
    28021 gggtatgatt tgcgtacttc tgccgaatgc gagaaaacgt tcgcggattt tgcccgtatt
    28081 gttggcttta agtatctgcg cgggatgcac cttaacgatg cgaaaagcac ctttggcagc
    28141 cgcgttgacc gccatcatag cctcggtgaa ggcaatattg gtcatgatgc gttccgctgg
    28201 atcatgcagg acgaccgttt cgacggcatt ccgctgatcc ttgaaaccat caacccggat
    28261 atctgggcag aagagatcgc ctggctgaaa gcgcaacaaa ctgaaaaagc ggtagcctga
    28321 agatgaacaa ccgggagaag gagatccttg caattttacg gcgtaacccg ctgattcagc
    28381 agaacgaaat tgcggatatg ctgcaaatca gccgttcgcg cgttgcggcg catattatgg
    28441 atttaatgcg caaaggccgg attaaaggca aaggttacat tctcaccgag caggaatact
    28501 gcgtagtggt ggggacaatc aatatggata ttcgcgggat ggcggatatc cgttacccgc
    28561 aagcggcttc tcatcccggt accattcatt gctcggcagg cggcgttgga cgcaatatcg
    28621 cccacaacct ggcgctgtta ggccgcgacg tccatttgct ttcagtgatt ggtgatgact
    28681 tttatggcga aatgctcctg gaagaaacgc gccgcgccgg cgtgaatgtc tccggctgcg
    28741 ttcgtttaca tggccaaagc acatcgacgt atctggcaat tgccaatcga gacgatcaaa
    28801 ccgtgctggc gattaacgat acccatctgc tggatcagtt gacaccgcaa ctactgaacg
    28861 ggtcgcgcga tttacttcgt catgcgggcg tggtacttgc tgattgtaac ctgacagccg
    28921 aggcgctgga atgggtcttt accctcgctg atgaaatccc ggtgtttgtc gataccgttt
    28981 cagaattcaa agcgggcaaa atcaaacact ggctggcgca tattcacacc ctgaaaccca
    29041 ctttaccgga gctggaaatt ttatggggac aggcgatcac cagcgatgct gaccgtaatg
    29101 ccgcagtgaa tgcgttgcat cagcaaggcg ttcagcaact gtttgtttat ttgcccgatg
    29161 agtcagttta ttgcagcgaa aaggatggag agcaattttt gctgacagcg ccagcgcata
    29221 cgacagtaga cagttttggt gctgacgatg gttttatggc gggcctggta tatagctttc
    29281 tggagggaaa caatttccgt gacagcgccc gttttgcgat ggcctgcgcg gcaatttcac
    29341 gcgccagcgg cagcttaaac aaccctaccc tgtctgccga taacgcgctt tcattagtgc
    29401 cgatggtgta acaatgttgc cggattcgac gcttgactac atcttatccg gctcataact
    29461 cgcgcactac gctaagccaa taaagaatcc tgcaatggtt gcactcatca ggttagaaag
    29521 tgtcgccgcc gccagcgcgc gtaaaccaag ctgggcgatt tccggtgcac ggtgtggcgc
    29581 aaccgcagaa aacgccccca ccaccacccc gatagaacca aagttagcga aaccgcacaa
    29641 cgcgaaggaa ataatcgcca cggttttagc atccagagtg ccagctgttt gcagataagg
    29701 tgagaaattg aggtaagcga caaattcatt tattgccagt ttctgtccaa tcaaactccc
    29761 ggcaagattc gcatcactcc agtcaacacc cattacccac gccagtgggg ccagcaggta
    29821 acctaaaatg gactccagcg aggcatgttc aaagccaaac cagccgccaa cgccgccgat
    29881 aataccgtta attaacgcaa tgatggcgac aaatgccatc actactgtcg ccacacctgc
    29941 ggcgattttc agcccggtca ttgcccctgt cgcagcggct tcaataatgc tttttggcgg
    30001 tgtttcggtg aaagagaggt tattaaagga aacctgcgaa gattctgttg ccgggcttaa
    30061 caggcgggca aacaagatcc cgccggggat cgccattaac gatgccgcca gcagatattc
    30121 cacaggcacg cccagtgcgg cgtaaccaat cattgtcgaa ccagcaatcg aggccatccc
    30181 gctacaaatc gctgtaaata attcattgcg attcagacga ttgataaagg atttgacgat
    30241 tgccggaatt tcgttttgcc cgaggaaaat ggtagtgacc gcgacgaatg actcgatctt
    30301 gctgatattc aatgctttct ggaagatacc gccgagaatg cgaattaaga tccccatcac
    30361 accgatgtag tagagaatac tcaccagtgc tgtaacgaag ataattgccg gtaataccct
    30421 gaaaccaaag ataaatcctg cgccatcaaa taaggtatcc atttttggtc cgaccagaga
    30481 accaaagata aatgcgctac ctgcatcgct gtacgccatc actttatgca cgccaaaagc
    30541 gactttttct gcgacccaac gccctggtgg taaccaaagc ataatgccgc caatcacgac
    30601 ctgtaacact aacgccgcgc caacggtacg caggctgatc ttcttcttgt ttactgacag
    30661 caaaaacgca attgccagca ataccaccat tcccagaaca cttctcatga catccatagt
    30721 tattatctct tcatgtcatt ttgggcgacg gctttctgcc catcagaaat agccgggcct
    30781 gcatcttgcg atactgtccc ggcttgttat caacacttaa tgggttttga tgtagccgcg
    30841 cacgcactct tcgactaatc cccagaacca gtcagtatca atagtgatgc cgactttggt
    30901 attggcgggc ttgccaagaa cgcccagctc gtcgcagacc gtacgcccgt agcaagggcc
    30961 gctgttaacg tccacttcga cgtacatctc ctgggtttta atgccatcag ggttaatcag
    31021 ataaccgatg caggtggcgt catgcaccgg gccgccagcc aggccgtagt tttcgaactg
    31081 ggttttgaga gtgaagttca tgatgtcgct gaacagctct ccggcagggc cgcctgccct
    31141 ttccatccga gcaatcacgt ccggggtgca aacggtctgg ttggtgagat cgaggcccat
    31201 catcactaat ggaacgccgg aggtgaacac cacgcgtgcg gcttccgggt cagcaaagat
    31261 gttgaactcg gcagatgggg tgaagttgcc agtaccgtaa gcgccgccca tcagcacaat
    31321 ttcacggatt ttcggcagga tcgcgggttg catacgcatt gccaccgcga tatttgaaag
    31381 tgggccaacc ggcaccaaag tgatatcgcc atcgcttgcc atcagggtat caatgatata
    31441 tttcaccgca tgagtgcttt ctgcctggcg ggtcagcggc tcgaataccg ggccatccag
    31501 tccggtttca ccgtgaatat tatcggcaac gatttgttga cgcataatgg gctgtggcat
    31561 ccccgcataa accggaacat taatctccag tttctggcaa acattcaggc cattaattaa
    31621 tgttttatca agcgtctgat tacccgccac aatggtgata cctaataaat caatcgccgg
    31681 atgtttcgct gccatcatta tggcaatagc atcatcatga cccggatcac aatccagaat
    31741 aatttttctc ttttccattg tttatttcct ctgtttccag ttgcgttatt ttttctacag
    31801 caaagaaaat tcgaccgggc cgatgattga atcttaacaa cagcgtaagt atgctaaata
    31861 tgagaaatct catagcggat aaacatcgtg aaagaaatcc acaataatga ccttaagcag
    31921 caattgatga gtgaatctgc gtttaaggat tgcttttcaa cggatgtttc agccgatacg
    31981 cggctgtttc attttttagc gcgtgactac attgtgcagg aagggcaaca gccgtcatgg
    32041 ctgttttatc tgacgcgagg ccgtgccagg ctttacgcca cgcttgctaa tggtcgcgtg
    32101 tcgctgatcg atttctttgc cgctccctgt tttattggcg agattgagtt aatcgacaaa
    32161 gaccatgaac cgcgtgcggt gcaggctatt gaagagtgtt ggtgccttgc gctccctatg
    32221 aaacattacc gcccgctgtt attaaacgac acgctatttt tacgaaaact ctgcgtcacc
    32281 ttaagtcata aaaattatcg taatattgtt tctttaactc agaatcaatc atttccgtta
    32341 gtgaatcgcc tggcagcttt tatattactc tcgcaggaag gtgatcttta tcacgaaaag
    32401 catacgcaag cggcagagta tttaggtgtt tcttatcgac atcttttata tgttctcgcg
    32461 caattcattc acgacggttt attaattaaa agcaagaaag ggtatctcat taaaaacaga
    32521 aagcagttgt caggactggc actggagatg gacccggaaa ataaattctc agggatgatg
    32581 cagtaaaaat tatttgcaat agagcgattg ccggatgtga cgcttaataa gcgcgtacat
    32641 ccggcaatga cactcaagct aaaccaataa agaacccggc aatggtcgcg ctcatcaggt
    32701 tggaaagcgt tgctgctgcc agcgcccgta aaccaagctg ggcgatttcc ggtgcgcgtt
    32761 ttggcgaaat agccgaaaat gccccaacga caacaccgat agaaccaaag ttagcaaaac
    32821 cacaaagcgc aaaggagata atcgcaatgg ttttcacttc cagcgtgccg cccgtttgca
    32881 ggtatgggga gaaactcagg taagcgacga attcgttaat cgccagtttc tgcccaatca
    32941 ggctacccgc aagattggca tcactccagt ccacacccat gatccacgcc agcggtgcca
    33001 gcacatagcc aaaaatactt tccagagagg cattggcgaa accaaaccag ccgccaattc
    33061 cgccgataat gccgttgatc agcgcaataa ttgcgacaaa cgccattaca accgtcgcca
    33121 caccagcggc gattttcagc ccggtcatcg caccgctcgc cgccgcttcg ataaagcttt
    33181 ttggcggcgt ttcgctgaac gacagatttt caaatgtcac ctgcgaaggc tcggtggctg
    33241 ggctaagaat acgtgcaaac aaaataccac cagggatcgc catcagcgat gccgctaaca
    33301 ggtagtcaat tggtacgccc attccggcat aaccaatcat catcgaacca gcaatggacg
    33361 ccatcccgct acaaattgcg gtaaacaact cgttgcgatt catgcgatcg ataaacggtt
    33421 taacgatcgc cgggatctca ttttgcccga ggaaaatagt ggtaaccgca acaaaagatt
    33481 cgattttgct gatgttgagg gctttctgga aaatgctgcc aaggatgcga atcagcagcc
    33541 ccatcacgcc aatgtagtac agcagactga tgagcgcagt gacgaagata atcgccggaa
    33601 gtacgcgaaa ggcgaagata aaacccgcac cgtcaaagag gacatccatt ttcggcccga
    33661 ccagcgaacc aaaaatgaag gcgctaccgg catcactgta agacatcact ttatgaacgc
    33721 ctaatgccgc ctgttctact gcccattttc ccggtgggaa gtagagcatg atgccaccaa
    33781 tagcgatttg cagcagcagt gcggctccaa ccgtgcgcaa actgatgctc tttttattca
    33841 ctgacaacag aaatgctatt gccagtaaca ccaccatccc cacaacactt ctcattatat
    33901 ccataatgat tttcccttca tgccagtaaa cccggcgtca gcgccaggtt ttggtatgct
    33961 tgatgagtac gggcgacggc tttctgcccg tcagaaatta acccgcgaga cgctggtatt
    34021 ctttggctat ttcactcgcc aggatggcgt tgttgaacac cagctggatg ttggatttca
    34081 ggctgtcacc gccggtcaac tcagcaacgc gcgccagcag gaatggcgta ctttctttac
    34141 caataacacc ctgcgcttca gcttcagcta ccgcctgatc gatcgccgca ttgatagtgt
    34201 gttccggcat agcaaactgt tccgggatcg ggttcgctac cacgaggcca ccgttcagcc
    34261 cgctttgcca tttcaccgcc attgcacggg caatttcgct ggcgctgtcg agacgaatgc
    34321 tcacgtcaaa cgggctggtg cggcagaaaa acgcaggcag cgctttggtc tgatagccaa
    34381 ttagcggtac accgaaagtt tctaaatatt cggtagttaa gcctaaatcg agaatagatt
    34441 tcgccccggc acaaacgacg gtgacattgg tgtgcgccag ttcttgcaaa tcggcagaaa
    34501 tatcgaaggt atgttccgcc ccgcgatgca caccaccaat tcccccggtg gcaaatactt
    34561 taattccggc aagcgccgca ataatcatcg ttgaagcaac ggtggttgca ccattttttc
    34621 cggcggcaac aacaaaaggt aaatcgcgac gactaacttt ggtcacgtta tgcccttcac
    34681 gacccagcaa ttcaatttct tctttgctta aacccacttt catcacgccg ccaataatgg
    34741 cgatagtggc aggtacagcg ccctgtttac gaatagtttc ttcaacttca attgcggtct
    34801 gggcattttg tgggaacggc atcccgtgag aaataatggt cgattccagc gccacaaccg
    34861 gttttttgtt ttttaaagcg tcctgcactt ccggggaaat ttgtaataat tcaggggaaa
    34921 tttttaattc agacattctg cgttctccac taacgatata acgttggcaa tcgataaatc
    34981 ggggttattg gtgtattcac aggagagcgc cattgacgaa catccctgtg cgaaacgaac
    35041 agattcggca aacggcattc cgtctaccca acacgaagca agtcccgcca tcatggcatc
    35101 gcccgctccg gtaacattaa tgacattggt tttgatcggc gcagaccaac cactttcacc
    35161 gctgatatca ctgtaataaa cgccgtcgcc gcccatgctc aataccagtc ggttcaggcc
    35221 atgttgatgg aaccaggcag caacttttgc cacatcttca cgccctgaca aagcaatccc
    35281 actcagggtt tccgcttcaa ggcggtttgg cttgagagtg tggatttgat ttagacggtc
    35341 gcgcactttg acacatttcc acgcggaaac aggatcgaca aatacgggta cgttggcggc
    35401 attatccaga atccatgcca gtgcctcttc actgatatta cagtccgcga caatgacctt
    35461 tgccctctga ataaattcac cgtgctgtgc gagatattca gctgtaatag cgttgctaat
    35521 attcatgtca tttatagcaa ccagcatttc accggtatta tcgagtaatg ataaatagct
    35581 cgacgtattt tctcccggca caatcaggca tttatcgaca taaacaccag attgattggt
    35641 ttgcgttagc agcgattgac cataaaaatc actgcctacg gcgctcagta gccaggcttt
    35701 attccccagc aacgccaggt tttgtgcaat attgcgccct actccaccag gcgtaaactt
    35761 tattttacct ggatttgaat ccgcataatt taatgattca tgtgaatatc cggcgacatc
    35821 aatattcgcc gaacctataa ttacgacata atccttttcg cgcatagcac gtcctctggt
    35881 aaaaaattac caatcctgtc aatccacaag aatatagata ctgcaaagac ggtttaaaca
    35941 tgtgttcata tttaaacatg tgctcatagt atgcatgttt tacgtaaagt aaattgccgc
    36001 cagagaggca aaatgggcag atttgagcag gctcacaaaa tgcgattaaa tgcctgaatt
    36061 acaaaagtga ggaatttgtg acgggatgca cagaacagga acactaaaaa agggcagaaa
    36121 ataatctgcc cttgaggaaa taaccttagt gtttgatgaa catttcaggt ttctactgaa
    36181 agataaaacc atttaaattc ataaaattac attcacaaac tggtcattcc ctttgcgctc
    36241 atcaaatgtt acagggcagg aaatttctgc cctgtaacac accttttatt acgctgcttt
    36301 cgctactgcg tccacttccg gacgtttcag gaaggcatag gccaaacccg ccaccagcgt
    36361 accggcaata attgctacca ggtaacccag taccggcgta atagcgccag ggatcagcag
    36421 aacaaacaga ccaccgtgcg gtgccataag tttcgcacca atcgccattg agattgcgcc
    36481 agtcagcgcc ccaccaacga tacaacacgg cagcacacgc atcggatcac gagcagcaaa
    36541 cggaattgca ccttccgaaa taaagcacag accaagaacc agagcggctt tgccaccttc
    36601 ctgctgcgct ttgtcgaatt tgcgacgcgc caccattgtt gccagaccca ttgccagcgg
    36661 tggcaccata cctgccgcca taatcgccgc catcgggcca taggtttgag tactcagcag
    36721 acccacaccg aatgcgtacg ctgctttatt taccggaccg cccatgtcag tacacatcat
    36781 gccaccgagg atcgccccca gcagaaccgc attcgcagtc cccatggtct gcagccagtg
    36841 agtcaaccct tcgagaatgc cagcaaccgg tttaccgatc aggtagatca tcgccagacc
    36901 gaccaccaga ctggaaatta gcggaatgat caggatcggt ttcagcgcct ccatactctg
    36961 tggcagtttc agttgcgtac tgattaactt cgcaatgtaa ccagccagga agcccgcaat
    37021 aataccccca atgaagccag aaccggtgct gaccgccagc atacccccaa tcagacccgg
    37081 cgtgagaccc ggacgatcgg caatggagaa ggaaatgtaa cctgccagta ccggcaccat
    37141 cagcgcaaag gctgaaccac caccaatctg catcagcgcc gctgccaacg tacccggctc
    37201 tttaaacgct tcgataccaa aagcaaaaga aagcgcgata cacagaccac ctgcaacgac
    37261 catcggcagc atgtaagaga cgcccgtcag caagtgacgg taagcgcctg caccctcttt
    37321 cttaccttca gtggtcgctg tttgagcttt gcccgccggt tcatacggcg ttgcttcagc
    37381 aaccgcttta tccagttcct gcgcggtttt cttcagcgcc agaccggtag aggtgcgata
    37441 catcggttta ccagcaaatt tcgccagatc cacttcgata tctgccgcca caatcaccag
    37501 atccgctgcg gctacttctt caggggtgat cgcattaccc gcgccaacag aaccacgggt
    37561 ttcaactttc acccaccagc cacgtttttt cgcttcggtt tcaatggctt cagccgccat
    37621 aaatgtgtgt gctacgccag tcgggcaagc tgtcaccgca actacgcgtt tcggaccgct
    37681 ggtggcaact ggtgctgtcg caacgaccgg tgcagtgtaa ggtttcgcat ggcctttggc
    37741 ttcactcagg aacagctcag ggtgcgcaac tgcccgggaa atatcgccca gccagacatt
    37801 tttaccgttc agcgcgctgt cgttcgggat ggaatcaccg agaacaatcg ccatttcagc
    37861 gtcgttcgga ttgtcgatga tttccagttt tgcttttcgc gccgccgcgc ccagcagggt
    37921 cttcgccata taggcgcgtg cctgaccgag attagcgtca ataatcagca gcgttttcat
    37981 tatgcctctc ctgctgtcag ttaaaaggtt gtaagtcgac gcgcgccatc attgcggcca
    38041 actgcggacg atcggtaata cccacattgc tttgacttac cgccagggct gcaacagctg
    38101 tcgccagacg cagtgtgtgt tcactggatt cacgcatcag caagccataa atcaggccac
    38161 caaccataga atcccctgcg ccaacggtgc ttacgacatc gactgacggt ggtttggcga
    38221 tccattcgcc ggaggcatta acccaaagcg cgccttcggc acccagtgaa ataacgacat
    38281 gcgcgatacc ttgttcacgc agcgcatgcg cagcttcaat cacatctttc atttcaggca
    38341 gtttacggcc tgcccagatt tccagctcgc ggcggttagg tttcaccagc cacggtgccg
    38401 ctttcaaacc tgctactaac gcttcacggc tactatcaaa gataatgcaa ggacactgac
    38461 tacgcaggcg agtcatccag tcggtgaacg cttccgggct gacgcctgac ggtaagcttc
    38521 cgctgacaca gaccatatcg aactgaccga gccagctcag agaatcagtc acaaagcgtt
    38581 cccagtcggc gggggtaacg tcaaaacccg agaagttgaa gtcggtcact tcgccgtctt
    38641 tttccgtcag cttaacgtta attcgggtgc gcccctgtac aacctggaaa cggttggcaa
    38701 tgcccagctc gctgaacagt tgctgaaaac catcctgatt gtctttaccg aggaagccac
    38761 caacggtgac atcaattccc aggtctttta atactttggc cacgttgatg cctttacccg
    38821 ccgcatgcag accggtggtt ttcaccaggt tcacttcgcc gcgttcaatt tccgggcaga
    38881 aaccaacaag gtcataagcc ggattaaggg tgatagtagc aacacgtctg ctcattatgc
    38941 gccctcccca agaccagcag cgatagcgtc gccgattgct ttcagcgcct gttcagcatc
    39001 tgcaccctgg gcggtaaagc gtaggcgatg acctttctta acgccaagtg ccacaacttt
    39061 catcagacta cgtccgtttg ccggtttgcc ggtaccatca aggtttgtca cggtaatatc
    39121 actgttaaat tgtttaatgg tattgaccag catggtacct ggacgagcat gcaggccgtg
    39181 ttcattgcgc accacaaact ccgcgcttaa cacgtcgtcg gtcggcgcat catcgctggt
    39241 cagcagcgcc agcaacgttg ccgcatccgc tttcagcaag cggtcagctt tattgtcgag
    39301 taacaaatca gcgagacgct taagaaccgc aatgggctga tcgtcattca tcgccacact
    39361 caccagcatg gctgccgttt cgccgtccac atcaaaagca tttgccgcac ggcttaccgc
    39421 aatcgcgcta cgcagattgc cttcggcgct atcgctcagc cagataccct gcccgaggtt
    39481 cagcggttgt tcattgatgg ctttggtgac gaaagtggcg tcaactgccc ccgcttcttt
    39541 cagacgcgca gcgttcagcg cctgaagagt cagcagatcg ctggcgacga tatccagcgt
    39601 cagcatttcg ttgtcgagct tcagctgctc actctgcttt tcgcccatca gcaatgcgcg
    39661 aagttcttct gctgttgttg ctgacttcag ttgttcagca acggaatcat cgctcagtac
    39721 gtgggtcagc tggcgtagca ggcccagatg ttcatccgag ctggcagcaa taccgatcgc
    39781 cacgtacgct acctggccgt caccccaggt gacgccttcc gggaactgaa atacctgaac
    39841 gccggttttc agcacctgat cgcgggtgtc ggtagtgccg tgtggaatag caataccatt
    39901 gccgaggaac gttgaggttt gctgttcgcg cgccagcatg ccattgacgt agccttcagc
    39961 tacattaccg gcctgcacca gcgccgcagc gacctggcga atcgcctctt ctttgtctcc
    40021 ggccttttcg cccggatgga tgtcctgtac ggataactgg aacatagttc tcctctcttg
    40081 ctgaattgaa acgattcagc ttctatgaga aaaaaagcgc caacctggct tagggttaaa
    40141 gacaagatcg cgctgaaacg tttcaagaga gcataatact tttgtttcag cacgcaagga
    40201 aagccgtaaa gttatgagca aaagtttgat gtgctgcaca attttttgtc atttttccta
    40261 attgctgatg ggaaaatccg ttatcagcaa tttcatttca gcagcgtcag cagccaatca
    40321 gcaacagtga aatggcattt tgattattcg tggcgctttt gatttcgctg tctgtttatt
    40381 ttctgacaag cagcgtaaac tccgcgtctt cctcttccag tgatcgacca gcatgcataa
    40441 ctcccccgca gtcaccagcg cgaaatcgtt tgacctgacc tcgacggcgt ttttaatcgt
    40501 tgcctttctc accggtattg cgggcgctct gcaaaccccg acactcagta tttttcttac
    40561 cgatgaagta catgcccgtc cggcgatggt gggattcttc tttaccggca gcgctgtcat
    40621 tgggattctg gtgagtcagt ttctcgccgg gcgctctgat aagcgcggcg atcgcaaatc
    40681 gctgattgtc ttttgctgcc tgttaggcgt gctggcctgc accctttttg cctggaatcg
    40741 caactacttt gttttgctat tcgttggcgt ctttcttagc agctttggct cgaccgctaa
    40801 cccgcaaatg tttgcccttg cccgtgaaca tgccgacaaa accggacgtg aggcggtgat
    40861 gttcagctct tttttacgcg ctcaggtttc actggcatgg gtcattggcc caccgctggc
    40921 ttatgcctta gcgatgggtt tcagctttac ggtaatgtat ctgagcgcag cggtagcgtt
    40981 tattgtttgc ggcgtgacgg tgtggctgtt tttaccgtca atgcaaaaag aacttccgct
    41041 ggcgaccggc acggttgaag cgccgcgccg taaccgtcgc gatacgctgc tgctgtttgt
    41101 catttgtaca ttgatgtggg gctcgaacag cctgtacatc atcaacatgc cgctatttat
    41161 tatcaacgaa ctgcatcttc ccgagaaact ggccggtgtg atgatgggga ccgccgccgg
    41221 gctggaaatc ccgaccatgt tgattgccgc agacattatt gtcgccaata aatccgaccg
    41281 tgcgacgccc gaaagtgagc aagcgctaca gcgctggtgg cagcaaaatg gtggcgatcg
    41341 gcaattaatt cacagcgagc atgggaaaat tgacggtcat cttctggatt tgccgcgtcg
    41401 caatttagcc gagttgcccg ccagcgccgc gcattctcat cagcatggcg tgaaaaaagg
    41461 gttagcagcg ttaagcctgc cagagcatca acgctggcgt cgcagtctga acagcgggca
    41521 aggatatcag gcctgtggct ggatattcga cgctgatacg gtattcgaca ccattggcat
    41581 tctggaatgg gcgcgacttg caccggtgga acgcgtcaaa ggcgtgatgc gtattcccga
    41641 agggctggtg cgaatcaacc gtcagggcga tgacctgcac attgaaacgc aaaacgttgc
    41701 gccaccggac agtcgtattg agctgatttc cagcagcgaa gctgactgga atgccttgca
    41761 gagcgcgctg ttgaagcttc gtttagcgac caccgcgtaa ggttgcctgc gtttttcagt
    41821 aagataatta gagaaaatat gattaaaaat ttgccgcaaa tagtgttgtt gaatattgtc
    41881 ggcctcgcgc tgtttctttc ctggtatatt cccgttaatc atggattctg gttgccgatt
    41941 gatgcggata ttttttattt ttttaatcag aaactggtcg aaagtaaggc ctttttgtgg
    42001 ctggttgcat tgaccaacaa tcgcgctttc gacggttgtt cactgctggc gatgggaatg
    42061 ttgatgctga gtttctggct gaaagaaaac gccccaggca gacgacgtat cgtgattatt
    42121 ggtctggtca tgctattaac tgcagtggta ttaaaccagc tgggtcaggc attaattcct
    42181 gtaaaacggg ccagcccaac attgactttt accgatatta accgcgtcag cgaactgctc
    42241 tctgttccca cgaaagatgc ctcacgagat agctttcccg gcgatcacgg tatgatgctg
    42301 cttatttttt cggcattcat gtggcgttat ttcggcaaag ttgcaggcct tatcgccctt
    42361 attatttttg tggtttttgc gtttcccaga gtaatgattg gcgcacactg gtttactgac
    42421 atcattgtcg gttcgatgac cgtgatattg atcggtttgc cctgggtgtt gctgacgcca
    42481 ttaagtgatc gattaatcac cttttttgac aaatcactac cgggaaaaaa caaacatttc
    42541 caaaacaaat aactcacagt aattaacatc atcagggtta tttttatagt gaggataatc
    42601 ctgatgatgc gcaccgtgct ttcatccatc gcgcgcaaaa atcattctct aagtaaatga
    42661 atggattgca tgcgtttcac tcaatcgtac tttaattgac caatcccgtt tattaacttt
    42721 ctgtatcact ttttcttata aaaaatcatg taaaaccgct cgccaagacc gcaccaatcg
    42781 ggtaatctcg aactcgtttt gcctcggcgg tagattatcc tcacagcata taattttgtg
    42841 cgttagtcca cagatttggc cttaaggaat tgtttcaaca tgcccaggta attagtctcg
    42901 tgtcgcttgg cattttttta taacgatatt tgtcgttaag gacttcaagg gaaaacaaac
    42961 aacatggtca aatctcaacc gattttgaga tatatcttgc gcgggatccc cgcgattgca
    43021 gtagcggttc tgctttctgc atgtagtgca aataacaccg caaagaatat gcatcctgag
    43081 acacgtgcag tgggtagtga aacatcatca ctgcaagctt ctcaggatga atttgaaaac
    43141 ctggttcgta atgtcgacgt aaaatcgcga attatggatc agtatgctga ctggaaaggt
    43201 gtacgctatc gtctgggtgg cagcactaaa aaaggcatcg attgttctgg tttcgtacag
    43261 cgtacattcc gtgagcaatt tggcttagaa cttccgcgtt cgacttacga acagcaggaa
    43321 atgggtaaat ctgtttcccg tagtaatttg cgtacgggtg atttagttct gttccgtgcc
    43381 ggttcaacag gacgccatgt cggtatttat atcggcaaca atcagtttgt ccatgcttcc
    43441 accagcagtg gtgttattat ttccagcatg aatgaaccgt actggaagaa gcgttacaac
    43501 gaagcacgcc gggttctcag ccgcagctaa taaaccgttt ggatgcaatc ccttggctat
    43561 cctgacgagt taactgaaag cactgcttag gcagtgcttt tttgttttca ttcatcagag
    43621 aaaatgatgt ttccgcgtct tgatccaggc tatagtccgg tcattgttat cttttaaatg
    43681 ttgtcgtaat ttcaggaaat taacggaatc atgttcatac gcgctcccaa ttctggacgt
    43741 aagctcctgc ttacctgcat tgttgcaggc gtgatgattg cgatactggt gagctgcctt
    43801 cagtttttag tggcctggca taagcacgaa gtcaaatacg acacactgat taccgacgta
    43861 caaaagtatc tcgataccta ttttaccgac ctgaaatcca ctactgaccg gctccagccg
    43921 ctgaccttag atacctgcca gcaggctaac cccgaactga ctgcccgcgc ggcgtttagc
    43981 atgaatgtcc gtacgtttgt gctggtgaaa gataaaaaaa cattctgttc atctgcgact
    44041 ggtgagatgg acattccact aaaagaattg attccggcgc tcgacattaa taaaaatgtc
    44101 gatatggcga tcttacccgg tacgccgatg gtgccgaaca aacccgcaat cgtcatctgg
    44161 tatcgcaacc cgttgttgaa aaatagtggc gtgtttgccg cgctgaatct caatctgacg
    44221 ccttctctct tttatagttc acggcaggaa gattacgatg gcgtcgccct cattattggt
    44281 aatactgcgc tatctacctt ttcttcacgt ttgatgaacg ttaatgaatt aaccgacatg
    44341 ccggtccgtg aaactaaaat tgcgggcatt cctctgaccg ttcggcttta tgcagatgac
    44401 tggacatgga acgatgtgtg gtacgcattt ttactgggtg gcatgagtgg aactttcgtt
    44461 ggacttctct gctattacct gatgagtgtg cgtatgcgcc caggcagaga aatcatgacc
    44521 gccatcaagc gcgaacaatt ttacgtggtg tatcaaccgg tggtggatac acaagctttg
    44581 cgggtaacgg gcctggaagt actgctacgc tggcggcatc cagtagcagg agaaatcccc
    44641 ccggatgcct tcattaactt tgccgaagcg caaaagatga ttgtgccact gactcagcac
    44701 ctgtttgagt tgattgcccg cgatgccgca gaattagaaa aagtactgcc ggtaggcgtc
    44761 aaatttggca ttaacattgc gccggcccac ttgcacagcg aaagctttaa agcggatatc
    44821 cagaaactgc tcacttccct gcccgcacac catttccaga ttgtgctgga aattaccgag
    44881 cgcgatatgc tgaaagagcg agaagccaca caactcttcg cctggctgca ttcggtcggc
    44941 gtagaaattg ctattgatga cttcggcacc gggcacagcg cgcttatcta tcttgagcgt
    45001 tttacgctcg attatctgaa aattgatcgt ggatttatca acgccatcgg tacggaaacg
    45061 atcacttcac ccgtacttga cgcggtgctg acgctggcga aacgcctcaa tatgctgacg
    45121 gttgctgaag gggtcgaaac gccagaacag gcacgatggc taagcgaacg cggcgttaat
    45181 ttcatgcaag gctactggat tagtcgcccg ttaccgctgg acgattttgt tcgctggctg
    45241 aagaaaccgt atacgccgca gtggtaaggt gtgcttacgt cccttattat tcatagtgaa
    45301 agcatgccgg attgcggcta atgatgagta aaaggaaatc cgttgcagat gattgtgcgc
    45361 atactgctgc tgtttatcgc tctgttcacc tttggtgcgc aggcgcagac tatcaaggaa
    45421 agctatgcct ttgccgtact gggcgaaccc cggtacgcat ttaatttcaa ccattttgat
    45481 tatgtgaacc ccgccgcgcc aaaaggtggg caaataacgt tgtctgcgct cggcaccttc
    45541 gataatttca accgctatgc actgcgcggc aatgcgggcg cacgcaccga gcagctgtac
    45601 gacacgctat ttacgacttc cgatgacgaa ccaggcagtt attacccgct gattgctgaa
    45661 agcgcacgct atgctgacga ttattcctgg gtggaggtcg ctattaatcc acgcgcccgt
    45721 tttcatgatg gttcgcccat tactgcccgc gatgtagagt ttacttttca aaaatttatg
    45781 accgaaggcg tgccgcaatt tcgtctggtc tacaaaggca ccaccgtcaa agccattgcg
    45841 ccgttaaccg tgcgcattga gttagcgaaa cccggcaaag aagatatgct gagcctgttt
    45901 tcgctgccgg tctttccaga aaagtactgg aaggatcaca aacttagcga cccgctcgcc
    45961 acgcctccgc ttgccagtgg tccgtaccga attacgtcct ggaaaatggg gcaaaatatt
    46021 gtctattccc gcgtgaaaga ttactgggca gcaaacttac cggtaaaccg tggacgctgg
    46081 aatttcgaca ccattcgcta cgattattac ctcgatgata atgtcgcctt tgaagcgttt
    46141 aaagccggtg cctttgattt gcgtttggaa aacgacgcca aaaactgggt cacgcgctat
    46201 accggtaaaa atttcgataa gaaatacatc atcaaagatg agcaaaagaa cgaatcagcc
    46261 caggatacgc gctggctggc gtttaatatc caacgtccgg tattcagcga tcgccgggtg
    46321 cgggaagcaa tcactctcgc ctttgacttt gaatggatga acaaggcgtt gttttataat
    46381 gcctggagtc gcacgaacag ttattttcag aataccgaat atgcggccag aaattacccc
    46441 gacgccgcgg agctggtgct tctggcacca atgaaaaaag atctaccgcc agaagtcttc
    46501 acgcaaatct accagccgcc ggtatccaaa ggcgatggct acgatcgtga caacctgtta
    46561 aaagccgaca aacttctcaa cgaagcgggc tgggtgctga agggtcagca acgcgtaaat
    46621 gccacaacgg gtcagccgct cagctttgag ttattgctac cttcgagcag caatagtcag
    46681 tgggtattgc cgttccagca cagcctgcaa cggctgggta tcaacatgga cattcgcaaa
    46741 gtggataact cgcaaatcac caaccgcatg cgcagtcgcg actatgacat gatgccgcgc
    46801 gtatggcggg cgatgccgtg gcccagttcc gatttacaga tttcctggtc atcggaatat
    46861 atcaattcca cttataatgc ccccggcgtg caaagcccgg ttatcgactc gctgatcaac
    46921 caaattattg ccgcgcaggg aaataaagaa aaattactgc cgttggggcg agcactggat
    46981 cgcgtattaa cgtggaatta ttacatgctg ccaatgtggt acatggcgga agaccgtctc
    47041 gcctggtggg ataaattctc ccaacccgct gtacgccctg tttacagcct gggtattgat
    47101 acctggtggt atgacgttaa taaagcgacg aaactgccgt cagccagaca acagggagag
    47161 tagatgggcg cttatctgat tcgccgtctg ttgctggtga tcccaacgtt atgggcgatt
    47221 atcactatca actttttcat cgtgcaaatt gcgcctggcg gtccggtcga ccaggccatc
    47281 gccgccattg agtttggtaa tactggtgta ttacccggtg caggcggtga aggtgttcgc
    47341 gccagccatg cacaaacggg cgtcggtaat atcagcgaca gtaattaccg tggcggacgc
    47401 ggattggatc cagaagtgat cgccgagatc actcatcgct acggtttcga taagccgatc
    47461 cacgaacgtt acttcaaaat gctctgggac tacatccgtt ttgattttgg cgacagcctt
    47521 tttcgcagcg cctcggtgct gacgctgatt aaagacagtc tgccggtctc catcaccctc
    47581 ggattgtgga gcacgctgat tatctatctg gtgtcgattc cgttaggcat tcgcaaagct
    47641 gtttataatg ggagccgttt tgacgtctgg agtagtgcat ttatcatcat cggctacgcc
    47701 attccggcct ttttgtttgc catcctgctg attgtcttct tcgcgggcgg cagctatttc
    47761 gacctgttcc ctctgcgcgg cctggtttcc gctaactttg attcgctgcc gtggtatcag
    47821 aaaatcaccg attatctgtg gcatatcacg ctgccggtgc tggcgacagt gattggcggc
    47881 tttgcggctc tgaccatgct gacaaaaaac tcattccttg atgaagtgcg taagcaatac
    47941 gtggtgacgg cacgtgcgaa aggggtaagt gaaaaaaata ttctctggaa acatgtgttc
    48001 cgcaacgcca tgctgctggt gattgccggt tttccggcga cgtttatcag catgtttttt
    48061 accggctcgc tgctgattga ggtgatgttt tcactcaatg gtcttggctt actgggctac
    48121 gaagcgaccg tctcgcgcga ttatcctgta atgtttggta ccttgtatat tttcaccctg
    48181 attggcctgc tgctgaatat tgtcagtgat atcagctata cgctggtcga tccgcgtata
    48241 gattttgagg gacgttaatg tcgcgactca gccccgtcaa tcaggcccgt tgggcgcgtt
    48301 ttcgtcataa ccgtcgcggc tactggtcgt tatggatttt cctcgtcttg tttggtttga
    48361 gtttgtgttc tgaacttatc gccaacgata aaccgttgct ggtgcgttat gacggcagtt
    48421 ggtatttccc gttgttgaaa aactacagcg aaagcgattt tggcggcccg ctggcaagtc
    48481 aggctgatta tcaggacccg tggctgaaac aacggctgga aaataacggc tgggtactgt
    48541 gggcaccgat tcgctttggt gctaccagta tcaactttgc taccgataag cccttccctt
    48601 ctccaccctc ccggcaaaac tggctgggaa cggatgccaa tggcggcgat gtgctggcgc
    48661 gcattctcta tggcacgcgg atctcggttc tgtttggcct gatgctgact ctctgttcca
    48721 gcgtgatggg cgtgctggcg ggggcgctac aaggctatta cggcggtaaa gtcgatctct
    48781 gggggcaacg ctttattgaa gtatggtcag ggatgccgac gctgtttttg attattttac
    48841 tttccagcgt cgtacagcct aacttctggt ggctgctggc aattaccgtc ttgtttggct
    48901 ggatgagtct ggtcggcgtg gtgcgggcgg agtttttacg tacccgtaat ttcgactaca
    48961 tccgcgcggc gcaggcgctt ggcgtcagcg atcgcagtat catcctgcgt catatgttgc
    49021 caaatgccat ggtcgctacc ctcacctttt taccgtttat tttatgtagt tcgataacca
    49081 ccctgacctc actcgatttc ctcggcttcg gtctgccgct cggttcaccg tcactcggcg
    49141 aactgctgtt acaagggaaa aataaccttc aggccccgtg gcttgggatc accgccttct
    49201 tgtcggtggc gatattgttg tctttgctga tctttattgg tgaagccgtc cgcgacgcat
    49261 ttgatcctaa taaggcggtg tagcatgacg caaactctgt tagcgattga aaatttgtcg
    49321 gtgggttttc gccatcagca aaccgtacgc acagtagtca atgatgtttc acttcagatt
    49381 gaggctggcg aaacgctggc gctggtgggt gagtcaggtt caggcaaaag cgttaccgcg
    49441 ctgtcaattt tacgcctgct cccttccccg ccggttgaat atctctccgg cgatattcgt
    49501 tttcatggcg aatcgctgct tcatgccagc gatcaaacgt tgcgcggtgt acgcggtaat
    49561 aagatcgcca tgatttttca ggagccgatg gtgtcgttaa atccattaca taccctggaa
    49621 aaacagcttt atgaagtact ttcactccac cgcgggatgc gtcgggaagc ggctcgtggc
    49681 gaaattctta actgccttga tcgcgtcggt atccgccagg cggcaaaacg gctgacagat
    49741 tatccgcatc agctctccgg cggcgaacgc cagcgggtga tgattgcgat ggcgctgtta
    49801 acgcgaccgg aattattaat tgccgatgaa ccaaccaccg cgctggacgt ctctgtccag
    49861 gcgcagattt tacagctgtt gcgcgaactg caaggcgagc tgaatatggg tatgctgttt
    49921 attactcata acctcagcat tgttagaaaa ctggcccacc gcgtggcggt aatgcaaaac
    49981 ggtcgctgtg tcgagcaaaa taacgccgct acgctatttg cctcacccac tcatccttac
    50041 acacaaaagc tactcaacag tgaaccgtca ggcgacccgg tcccattgcc agaacctgcc
    50101 tcaacgttgc tggatgttga acagcttcag gttgccttcc ccattcgcaa agggattttg
    50161 aagcgcattg tggatcataa tgtggtggtg aaaaacatca gttttacgct acgagcgggt
    50221 gaaacactgg gtttagtggg cgagtctggt tccgggaaaa gcacgacggg actggcgctg
    50281 ctgcgactga ttaactctca gggcagcatc gtctttgacg gtaagccact gcaaaattta
    50341 aatcgccgcc agctgttacc tattcgtcat cgcattcagg tggtatttca ggatccaaac
    50401 tcctcgctca acccacgact caacgttttg cagattattg aggaaggctt acgggttcac
    50461 cagccgacgc tttctgccgc gcaacgcgaa caacaagtga tagccgtgat gcatgaagtg
    50521 ggattagatc ctgaaacacg ccaccgttat ccggcggagt tctctggtgg tcagcggcaa
    50581 cgtattgcga ttgccagggc gttaattctt aagccctcgc tgatcattct tgatgaacca
    50641 acatcatcac tcgacaaaac ggtgcaggcg caaatattga cgctattgaa atcattgcaa
    50701 caaaagcatc aactggccta tttgtttatc agccacgatt tgcacgttgt ccgcgcgtta
    50761 tgtcatcagg ttatcgtact gcgacaaggg gaagtagtgg aacaaggacc gtgcgcgcgc
    50821 gtgtttgccg caccgcagca ggagtatacg cgtcagctac tggcgttgag ctgacgctta
    50881 aaaaggattg tagtttgaaa agggttcggc aattgccaca ccaaaatttt tcaatcggca
    50941 tgttgcagca aattcgtcct ggcgatttac aaacaggcac ggctcacctt cacactccag
    51001 caccgaacat ggaacttcga tgtcgcttaa cgcctggctt agtttgtaca tcaccgacca
    51061 tgcattatca ccatccgggc taagcaattt aagcgccacc aggctgttgt caccgcacgg
    51121 tccattttgc ggaatcggtt caaccttcga acctgcgaaa ccagcagacc agcgatagtt
    51181 ctgcggcagt cgatggacga ttgagagttg taatgaagtc acttattttc cccggaagta
    51241 cattttactt cacaatttgt ttacatcaat tttaacacat catcaacaat ccgtctttat
    51301 acagatcgta aagattgatg cctcgcctgc gtggcctcta tggctctgat ttaaataata
    51361 tctgcgggcg tggtcacgtt tgcggggcta tatgtacccg tttctgcgat ctaactcaac
    51421 ctttttaact acaatgatgt gactttttac ataaattgat tttacataaa ataaacatat
    51481 atcgggggaa tgatagattt gtggttgaca tgcatcaaca aaggaagcct tttagcttcc
    51541 tcgttgtgca atagatcacc gttttttcgg ccgactggcg tacaaacaga agagaatgga
    51601 gctggttgcg cagaatgcaa ttgaccaaat catcggccat gcagagttaa aggtcgcaag
    51661 agaaagtaat gcgccaacaa ttgcccctat gccgaaacgg aaggttcctg ccagcgaaga
    51721 tgccgttccc gccatatggg gaaactcatc aagaatgacc gccatcgcat tggatgacac
    51781 catcgacacg cagcccacaa acgccgcaac gccaaccacc agcgaccaga atcccagccc
    51841 cagcagtgca ctgatgacca tccacgctgc cataataaac tgtatccaca accccgagcg
    51901 gaacatattt aacgcgccaa tacggcggac gaagcggctg ttaaagatgg tcatcacgaa
    51961 cagaaaaaga atgtttagcg caaagtaata accaaagttt tccggcgcga cgtggttgat
    52021 ttcaatgtag acaaacggtc cggcgcttaa gaatgagaac atcccggcaa agctgaaacc
    52081 gctggccagc atataactca ggacacgctt atgacggaac agcgccgcaa agttaccaat
    52141 agtggtacga atgtgaaaag gctggcgacg ctccggtggt aaggtttctt tgatcagaaa
    52201 gaagatcatt gccgaagcca gaatcgccgc cacggcgagg atccagaaga tgtaatgcca
    52261 gctcagccac accagcaccc agccgccaac tatcggtgcc atcagcggtg caatggtcgt
    52321 caccagcatg acaaacgaca tcatccgtga gaactcttct ttcgggtaga tatcacgcat
    52381 caaagcgttg ataaccacgc tggccgcagc cgcagccagc ccgtggaaga aacgcatcac
    52441 aatcagctga tcgatggttt gtgccaacgc acacgccacc gcggcggcgg caaacaccag
    52501 cgtaccgcca agaaccaccg gcttacgccc gaagctgtct gccatcggcc cgtagattaa
    52561 ctgccccaac gcaaagccca gaatataagt actgagggtc atctgcgtac tgcccgccgg
    52621 tacaccaaac tgagctgaaa ttaccggtag cgcaggcaga tacatatcaa tcgacagcgg
    52681 catcaacatg gccagcaggc caaggataaa aacaatagca aacgacgaat gctgtcgggt
    52741 ggtcacaacg ggctcctgaa agtcattgaa aagttagacg acgctggcaa tttcttcttc
    52801 agttaacgga cgatattcac cgggagctaa atcagcatcc agcgtaatgc cgccaatacg
    52861 ttcacgatgc agctcaacca catggttacc cacggcggcg aacatgcgtt tcacctgatg
    52921 ataacgccct tcgctgatgg tcagacgaac ctgcgttggg gtaatcactt ccagcaccgc
    52981 cggcttagtg agatcttttt cgttatgcag ctgcacgcct ttagcaaatt gctctgccgt
    53041 atcgtcagct acgggtgatt ccagcgtcac cagataggtc ttctcgcaat gatggcgcgg
    53101 ggaagtaatg cggtgcgacc actgaccatc atctgtcatt agtaccagac cggtggtatc
    53161 gatatccaga cgccctgcag cgtgcagttt ccacgctacc ggttcatcaa gaaaatagag
    53221 caccgttggg tgatccgggt cgtccgtgga gcaaacatag ccctgaggct tattgagcat
    53281 gaagtaacgt ggaccgtgtt gctgcgccag cgggttgcca tcgtaagcga catcatgttc
    53341 agggagcagt ttgaacgctg cattacggac gatttcgcca tcgacggtga cacgattgcc
    53401 gcggatttca cgcccggcaa tagcacggct aacgccgagt tgctgtgcga taaatttatc
    53461 aagtcgcatg tgtgtgattt tgcctgtaaa aaacggaggt cgggtaatga cccgaaaatc
    53521 tgaactgttg tctgcccagt atagcggtct aacaacatcc ctcaagggaa aaagatccat
    53581 ggcatactat tagcagaata atctacctac gcgagaccat gatttttaca cttcgcccat
    53641 atcagcaaga agccgtggat gccacgctca accattttcg tcgtcataaa acccctgccg
    53701 ttatcgtgct gcccaccggt gcaggtaaaa gcctggtgat agcggaactg gcgcggctgg
    53761 cgcgtggtcg cgtgctggtg ctggcacacg ttaaagaact ggtggcgcaa aaccatgcaa
    53821 aatatcaggc gctggggctg gaagccgata tttttgccgc cgggctaaag cgcaaagaga
    53881 gccacggtaa agtggtattt ggcagcgtgc agtcggtcgc ccgtaatctt gatgcctttc
    53941 agggtgaatt ttcgctgttg attgtcgatg aatgtcaccg tattggtgac gatgaagaga
    54001 gccagtatca gcaaatcctc actcacctga ccaaagtgaa tccccactta cgcctgctgg
    54061 ggctgactgc cacgcctttt cgactgggca aaggctggat ttatcagttc cattatcacg
    54121 gcatggtacg cggcgatgag aaagcccttt tccgtgactg catttatgag ctaccgctgc
    54181 gttatatgat taaacacggc tatctgacgc cgccagaacg actggatatg ccagtagtgc
    54241 aatacgattt cagccgcttg caggcacaga gtaacgggct attcagcgaa gccgatctca
    54301 accgcgagct gaaaaaacag caacgcatta ccccgcacat catcagccag attatggagt
    54361 ttgctgtaac gcgcaaaggg gtgatgattt tcgccgccac cgttgaacac gcaaaagaga
    54421 ttgtgggact gctgcctgcc gaagatgctg cactgattac tggcgacacc cccggcgctg
    54481 agcgcgatgt gttaattgaa gattttaaag cccagcgttt tcgctatctg gtcaacgtcg
    54541 cggtactgac caccggattt gacgccccgc acgtcgatct tatcgccatt ctgcgcccta
    54601 ccgaatcagt gagtctttac caacaaattg tcgggcgcgg tctgcgtctc gctcccggca
    54661 agactgattg cttaattctt gattatgcgg gtaaccctca cgatctctac gcgccggaag
    54721 ttggtacacc gaaaggcaaa agtgacaacg ttccggtaca ggttttctgc cctgcctgcg
    54781 gttttgccaa caccttttgg gggaaaacga ccgccgacgg gacattgatt gaacactttg
    54841 gtcgtcgctg tcagggatgg tttgaagatg acgacggaca tcgcgaacag tgtgacttcc
    54901 gtttccgttt taaaaattgc ccgcaatgta acgctgaaaa tgatattgcc gcccgccgct
    54961 gccgcgaatg tgacacggta ctggttgatc cggatgatat gttaaaagcg gcgctacgac
    55021 tgaaagacgc gctggtatta cgctgtagcg gcatgtcttt gcagcatggg cacgacgaga
    55081 aaggcgaatg gttgaaaatc acctattacg atgaagacgg cgcggatgtg agtgagcgtt
    55141 tccgtctgca aacgcccgcc cagcgtaccg ccttcgaaca actttttatc cgtccgcata
    55201 cgcgcacacc gggcatccct ctgcgctgga tcaccgccgc cgatatcctc gcccagcaag
    55261 cattactgcg acacccggat tttgtcgtcg cccgcatgaa aggtcagtac tggcaagtgc
    55321 gtgaaaaagt gttcgattac gaaggtcgtt ttcgtcgggc gcacgaatta cgcggttaat
    55381 ggtagttttg attgatgtat aaggcgattg agtatagaat ctcgcccgct tttgcatacg
    55441 caaagcagat cacttacctg ttgctgggtc gcctgtagca ggattaattt aagagagaaa
    55501 gaaatgttta ctatcaacgc agaagtacgt aaagagcagg gtaagggtgc gagccgccgc
    55561 ctgcgtgccg ctaacaagtt cccggcaatc atctacggtg gcaaagaagc gccgctggct
    55621 gtcgaactgg atcacgacaa agtcatgaac atgcaagtta aagctgaatt ctacagcgaa
    55681 gttctgacca tcgttgttga cggtaaagaa atcaaagtta aagctcagga cgtacagcgt
    55741 cacccgtaca aaccgaagct gctgcacatc gacttcgttc gcgcttaatt gctgaataag
    55801 ttgtaaaaaa ccccgctccg gcggggtttt ttgtctctgc aaattatgcc tgattacggt
    55861 attgctattt tttgcaggcc agataaggcg ttcacgccgc atctggcatt cagaacacaa
    55921 aaccgattaa ttgccgccag acgtccggcg ctgcaattga tcgcgcaagt tcggcggcgt
    55981 gcctttaatg gtcagggtat cggttgctgg atcccagaag atgcgttcac ctaacagcat
    56041 cgcatcaaag ttaatagtca agccaccgcc gctaccggca aacttcgtca actgacgcag
    56101 tgtgctacgg tctgccggga agctctcttc cagttcgtag cctttttcag ccgcaaactc
    56161 cgtgaagctc acttcgctga cgcctgccag ctctttcgac agcgattcca gttcaatctc
    56221 ttcacctgcc tgcagctgct cattacagta gctataaacc tgctggcgca cgttctgtcg
    56281 ctctgcttta tccagttgtg cttcggcagt gaaatcatcg accgcttgca acagcccacg
    56341 gttttgcgct ttcgcgttta acccttcgct ggcaccgagg aaatccataa agaaatccgc
    56401 cactttgcgc cctactcgcc ctttcaggaa agtgagatag cgggtcgact ctggattggt
    56461 ttcccactcg gttaaatcga tacgcgcaac aatatccgca tgattgatat caagataatg
    56521 ggttgggttg atgtcgagat tttcgttaac acgcatactg ctcaggttgc tcagaactgc
    56581 caccagcaga tactccaccg ccagatagcg atagtggcaa aacagcacga atccaccgtc
    56641 agcaaaagga tatttcgcta attcgtcacg caggcgaccg gttgccgcgc ggctaaatgc
    56701 cagaaagtcc tcttcgccct gacgctgtaa gcgcagcgtc tgcgccagtt cactctcttc
    56761 gctaaacaag ccgtaagctt tatttttggc gctatagacc cgatgcagtt ctgccaccat
    56821 ctcaacgacg gtttccgtcg gttccagcaa tgaatcgcgc aacaccagct caaggttctg
    56881 ctcatcacgc ttgataagct ggtgcagagc aatctggttg atatccagac tcatgataaa
    56941 ctctccttta agaccgggcg gtattcaacc accgccggtt aagcgacgca ataaaagatg
    57001 gcggtcattt tacagaaggc ggcgtaacga gaatgattta acgcataaaa aagcagaaaa
    57061 aaaaccgttg ctacggtaat atgttgccct ttcatcaact aactgatttc gatttatgcc
    57121 acaaatttcc cgctacagtg atgaacaggt tgaacaattg ctcgcagagc tgctcaacgt
    57181 actggaaaaa cataaggctc cgaccgacct ttccctgatg gtgttaggca acatggtcac
    57241 taaccttatc aacaccagca ttgccccggc ccaacgccag gcaattgcca actcttttgc
    57301 ccgcgcctta cagtcctcta tcaacgaaga caaagcgcac taagggaaac agataacagg
    57361 ttatggtaac tcatcgtcag cgctaccgtg aaaaagtctc ccagatggtc agttgggggc
    57421 actggtttgc actgttcaat attctgcttt cgctcgtcat tggcagccgt tacctgttta
    57481 tcgccgactg gccgacaacg cttgctggtc gcatttattc ctacgtaagc attatcggcc
    57541 atttcagctt cctggtgttc gccacctact tgctgatcct cttcccgctg acctttatcg
    57601 tcggctccca gaggctgatg aggtttttgt ccgtcattct ggcaacggcg ggaatgacgc
    57661 tattactgat cgatagcgaa gtctttactc gtttccatct ccatcttaat cccatcgtct
    57721 ggcaactggt tatcaaccca gacgaaaatg agatggcgcg cgactggcag ctgatgttca
    57781 tcagcgtgcc ggttatttta ttgcttgaac tggtgtttgc gacgtggagc tggcaaaagc
    57841 tacgcagcct gacgcgtcgt cggcgcttcg cgcgcccgct ggccgcattc ttatttatcg
    57901 cctttatcgc ctcgcatgtg gtttatatct gggccgatgc caacttctat cgcccgatca
    57961 ccatgcaacg cgctaacctg ccgctttcgt acccgatgac ggcgcgacgt tttcttgaga
    58021 agcatggtct gcttgatgcg caggagtatc aacgccgtct cattgagcaa ggtaatccag
    58081 acgccgtttc cgttcagtat ccgttaagcg aactgcgcta tcgcgatatg ggcaccgggc
    58141 agaatgtgct gttgattact gtcgatggcc tgaactactc acgcttcgag aagcagatgc
    58201 ctgcgctggc aggttttgct gagcaaaata tttcgttcac gcgccatatg agctccggca
    58261 acactacaga caacggcatc tttggcctgt tctatggcat ctcgccgagc tatatggatg
    58321 gcattctgtc gacccgtacg cctgcggcat taattactgc gcttaatcag caaggctatc
    58381 agctggggtt attctcgtca gatggcttta ccagcccgct gtatcggcag gcattgttgt
    58441 cagatttctc gatgccgagt gtacgcaccc aatccgacga acagaccgcc acgcagtgga
    58501 tcaactggct gggccgctac gcacaagaag ataaccgctg gttctcgtgg gtctctttca
    58561 atggcacaaa cattgacgac agcaatcagc aggcatttgc acggaaatat agccgggcgg
    58621 caggcaatgt cgatgaccag atcaaccgcg tgctcaatgc actgcgtgat tctggcaaac
    58681 tggacaatac ggtggtgatt atcactgccg ggcggggtat tccgctgagc gaagaggaag
    58741 aaacctttga ctggtcccac ggtcatctgc aggtgccatt agtgattcac tggccaggca
    58801 cgccggcgca gcgtattaat gcgctgactg atcataccga tctgatgacg acgctgatgc
    58861 aacgcctgct acatgtcagc acacctgcca gcgaatattc gcaaggtcag gatttgttca
    58921 accctcaacg ccgccattac tgggttaccg ccgcggataa cgatacgctg gcaattacca
    58981 ccccgaaaaa gacactggtg ctgaacaata acggtaaata ccgcacttac aacttacgtg
    59041 gtgaaagagt gaaagatgaa aaaccacagt taagtttgtt attgcaagta ctgacagacg
    59101 agaagcgttt tatcgctaac tgattaatta taaatcagtt agcaaaatat cttacttgca
    59161 atcggtgtgg aaaacggtag tattagcagc cacgagtcgg cacgtagcgc agcctggtag
    59221 cgcaccgtca tggggtgtcg ggggtcggag gttcaaatcc tctcgtgccg accaaaaatc
    59281 ccaagaaaac caacctattg cggttggttt ttttatatct gcatttaatt cgataaacag
    59341 acagcgacac atcacgacct gtttattttc tgttatcaga acgtccagac tacacccgcc
    59401 tgagttgtgc tgctttcttc acgggagaaa cgactatcga taccgagcgt caggcccgtc
    59461 aatgtttgct caaagccagc tcccgcatca gtgttcacgt tgttgcggct gttaattgcc
    59521 gaactccaca tcgccgccct ttacgctacc aagacgctca cgcacggtgt ccagttctgc
    59581 atcaaatacc agcggttgtg ctgccgccat attcagcaca tccgtggttg aagggggcat
    59641 ttgtacgcga ttctctgtga gactccagct atggttgcca ttatccagca aggtatattc
    59701 atacgtactg atatcaacaa cgcttccggc attgcccaac gtaaatgccg catcaccgcg
    59761 ccctccttta atcgtgggat cagtcgccgc gccataaacc tgtagcactc cattattgat
    59821 cgtagtctgg tttaccgcgc cacccatttg aacaatttga taaccatcat ttaatactga
    59881 gtattctgcg gtgccattta tttcaatgta ctgatatccc ccgttaattt tagtattact
    59941 acttacacca aattcttata tatgctgttc tccgccagga ttgattttcg tattattggc
    60001 ggccccataa accagctgga tatctttttc caggacaaca ccatcaactg tctcaccata
    60061 ctcaacagtt gatgccaatg ctatgggtga atataacgct gtagccacca gaacagatat
    60121 tgcggaacga caaagagaaa cagaaccaga ttggtgcatt aagctttcat cctatgaaat
    60181 taattgctgt taaaagcatt ggggacagaa aatacccata gctccatacc cggagtcagt
    60241 tttaaaaaac tgtttaaaga aatgcacaag tattgtgatt gattttttag ttgtttttcc
    60301 tgatgagaag ctgatgcaaa attccgtctt tataatgaag atgatgccaa agcgaacgac
    60361 aaagttgtag ttttcactac atgtccatac ataaaatggg gtaacattca cgcgcctggt
    60421 agcgttacca acgctacgct caaacataat gattctaata aaacctcagg agactactat
    60481 gcctgaagca acgccttttc aggtgatgat tgtggatgat catccactta tgcgacgcgg
    60541 tgttcgtcag ttactggagc ttgatcctgg ctttgaagtg gtcgccgaag cgggcgacgg
    60601 cgcgagcgcg atcgatctgg cgaatagact ggatatcgac gtgatcttgc tggatctcaa
    60661 tatgaaaggt atgagtggcc tggataccct caatgccttg cgcagggatg gcgttaccgc
    60721 gcaaattatt atcctgaccg tatccgatgc ctccagcgat gtctttgcgc tgatagacgc
    60781 aggcgcagac ggttatctgt tgaaagacag cgacccggaa gtgttgcttg aagcgattcg
    60841 tgccggagcg aaaggcagca aagtttttag cgaacgcgtc aatcagtact tacgtgaacg
    60901 tgaaatgttt ggcgcggaag aagatccctt cagcgtgctg acggagcgcg agctggatgt
    60961 tctgcacgag ctggcacagg ggctgtcaaa taaacagatt gcctcggtgt tgaatatttc
    61021 cgagcagaca gtaaaagtac acattcgcaa tctgctgcgt aaactcaatg tccgctcacg
    61081 cgtggcggcc accattctgt tcctgcaaca acgcggggca caataaaaat agcccgatgg
    61141 catcaccatc gggccacttt ttatttactc tcctgcggcg acaaatgttg catcgcctgc
    61201 gcgatactac gttcgatcac cgcacggcga gtatcgttgg caggtaagag tttcaacatc
    61261 atttcccacg cggcaacggc ttcgccaaat cgttgctgct caaaggcatt aaatgcatac
    61321 atgcttaaca cacggatatt gctatggtcc gttctcacca actggcgcag cagttcgccg
    61381 ccgaggcggt tgtcgttggg atcagatgaa cgtgtcaacg cttcagcgta tccgagcgcc
    61441 gcatcactgt tcttcggatc gaggcgatat gcagtagcgt atgcatcggt ggcgatactg
    61501 gcgttaccca gcgccatgcc aacgcggccc aacataatcc agccttctat atctcccgga
    61561 tttttttgca gttgagtacg catccccagc gcaaggcgcg acatctcttc ttcgttgagc
    61621 ggagcggctt tcggatccag cgccctgtcc agtaacgccg gagcctgcgc ggtggcctgc
    61681 tgccagattt tcacctgctg ataattgcca gtctggtagt agctgacgcc agccacaatt
    61741 aacgccacca caatacccgg cagataaaca acatatccgg cacgcttacc ttccggcacg
    61801 ctttgttctg gaaacgcttc cggcaccacg cgtacccgac gccgcgaacg ggcgtatatg
    61861 acccaaccgc caatgccaat agccactact ggcagcaccc acagcagcac ggtcagcggc
    61921 gttaacggcg gatcgtaagt gacgaagttg ccgtaacgcg ccaccatata atcgacaatc
    61981 tctttcttac ttttgccttc ctgcatcagt tcatacactt tctggcgcag gtcggtggca
    62041 atcatcgagt tggaatcggc aatgctgttg ttctggcatt tcgggcagcg cagttcttca
    62101 gtgagctgac ggaactgttg ttcctgtgct tcatctttaa actgcaacac gtcgatggtc
    62161 gccagcgctg agccggagat catcagcatc agcacgccca ataaaaacct cattgtgcgg
    62221 cctccttact gtatttctcc cacagtggct tgatctcttc ttcccagacg cgaggattga
    62281 gatcgcccgc atggcgatag cgaatgatgc cgttgccgtc aataaggaac gtttctggcg
    62341 cgccatagac accgagatcc agccctaaca tgccgtcgcc atcaaacagg cttagtgcgt
    62401 aaggattgcc caactctttc agccagctga ttgccttctg gcgatcgtct ttatagttca
    62461 tgccgaccac gcggatgccc tgcgcagaaa gctgattcag atattgatgt tccgcacggc
    62521 aggtcggaca ccaggtcgcc cagacgttaa gcagcaccgg tttgccctga gtcagcacat
    62581 ccgcctgata aaactgcccc ggattgtcca gtgattcgag gcgaaacttc ggcactagct
    62641 tgccaatgag cgccgattcc agattggtcg gatcatcccc ttcggcatta cgcgccagct
    62701 gccacagtag cgccgcggca atcgccagga agataatcaa cggaattaac aatactttgc
    62761 gcttcataca gcctccggcg cagttttctg cgggttcacg cgcttacgat agcgaggatc
    62821 aaacaggcac agcagcccgc ccaatgccat catcagcccg cccgcccaaa tccagcgaac
    62881 aaacggtttg tagtaaagac gcacggccca cgcgccgttt tccagctctt caccgagggc
    62941 cgcgtacagg tcacgcgtga tgccgccgtc aattgccgct tcggtcatca tcgacccggc
    63001 agtgttgtaa taacgttttt ccgcatacag cactgtttcc ggcttgccat ttcgcgttac
    63061 gccgatagtc gccacaccgc cacgccagtt cggaccagtc acctctttga catcacggaa
    63121 ggtgaagcga tattcatgaa tatcgacgct atcgccggac ttcatgcgca catcacgctc
    63181 aacgctatag ttctggctaa aggcaatacc aacaattgtc actgccagcc ccaggtgagc
    63241 cgccaccatt ccccaataac tgaaggtggt tttcgtgcca cgtgaaatac gcagcgcagc
    63301 ttccgcaatt gccagcatcg caatccagca ggccattgcc aggccgagca ccgtcatcgc
    63361 cacaactttg ctttcgaaca gccacggcag cagcagcgac agcaccagcg ttgagatgaa
    63421 ggcgataatc aataaattgc ggattttacg tgggcgatcg cgcccccagc gcaccagagg
    63481 accgacacca agcagcagcg caaacggcac catcagccag gtaaacatgg tgttgaagaa
    63541 cggttcgcca atcgaaatac tgcccagtcc cagttgcttg tgcaccagcg gcagcagcgt
    63601 ccccagtaat accaccagca tcgcggcgac cagcaaaacg ttgttcgcta acagcagaga
    63661 ttcccgcgac cacagcgcat tgtttacgcg tgagcgaact ttgtgtccac gcgcggcaaa
    63721 cagcagcagc gaaccgccaa tcaccaacac cataaaggcg aggataaaca taccgcgcgc
    63781 cggatcagac gcaaacgcgt gtaccgatac cagcacgccg gaacgcacga ggaaagtccc
    63841 caacagacac aacgagaagg cactgattgc cagcagtaat gtccacgctt tgaagctggc
    63901 gcgttgttca gtgaccgcca gtgagtgcat cagcgcagtc cccaccagcc acggcataaa
    63961 cgaggcgttt tctaccggat cccagaacca ccagccgccc cagccgagtt cgtaataggc
    64021 ccatgcggaa ccgagcacaa tgccgagcgt caagaagatc cacgccgcca gcgtccacgg
    64081 gcgggtaaaa cgcgcatagg tgctgtccag acgcccactc aacaaagaag caatggcgaa
    64141 agcaaacgcc accgagaaac cgacgtaccc catatagagc agaggcggat ggaagatcag
    64201 ccccggatcc tgcaacagcg ggttaagatc gcgcccttca atcgggaaat tcggcagcgt
    64261 gcgtgagaac gggttagagg taaagagaat gaacagcaaa aagccgacac tgaccatccc
    64321 cattatcgcc agtacgcggg ccacaatatc cagcggaata cgctgactaa aaatcgctac
    64381 cgcaaaggtc cagccgctca tcagtagcac ccacagcagg agcgagcctt catgcgcgcc
    64441 ccaggtagcc gccacgcgat accacaccgg aagctgggta ttggagttgc tggcaacata
    64501 ggtgacggta aagtcgttga ccacgaaagc attgaccagc accagaaatg cgccagccac
    64561 agacataaac agcagccagg caaacaagcg ggaagacgcc atcatgcgcg cgtccccgcg
    64621 cgccacgccc catagcggat acacagacag cagcagcgca attcctagcg ccagacacag
    64681 cagcccgtta ccaatttctg gcatcatgat gctgggtcct tataaacact cgccgggcga
    64741 cggtggttag cttccatcgc tttctcaact tctggcggcg tgtagttttc atcgtgtttc
    64801 gccagcactt ctttcgcgag gatatgattg cctttttcca gctcgccctg caccacaacg
    64861 ccctgccctt cacggaacag atccggcaaa atgccttcgt aagagacatc tactgaacct
    64921 tcagcgtcgt aaatggtgaa agttactttt agcgaattgg gatcgcgctg cacactcccc
    64981 ggcatcacca tcccgccaac gcgcagacgc tgaccgactt ccggcatctg ctgagtttca
    65041 cgcttgccgt agagaatctc ccccggcgta taaaagagat cgatattcga gcgcagcgca
    65101 tatagcacca gaccgatagt cagcgccagc cccgccagca cggcacaggc aatccacaag
    65161 cggtttttac ggcgaatatt catgcagcct cctgctgttg cgcagcacgt aaacgcgcct
    65221 cacgcgcccg ctgttgcgcc acgccacgca gaattgcgcg atgctgcatc accgagtgca
    65281 cgaccaaaat caccagcgga ataacggtca tcaccaccgc cagccagaca aaaaaggcgt
    65341 aaccgcccat tgcgaaaaat tcattccagg aagcaaatgc aggggtcatt tacggcctct
    65401 tttcagtatc agttcactca cccacggacg gcgtttttcc atcagcaaaa tcaaattacg
    65461 catccgcatc agcgtcagcg tggcagacag cagcaggaag ccaaaaatcg accagcgcag
    65521 cggcgaacgc atcgccggat cgatactttg ttgcatccgc gttgatccct gatgcagggt
    65581 gttccaccac tccacggagt aatgaataat cggcagattc accacgccaa tcagcaccag
    65641 aatacctgcc gcacggcccg ccagacggcg gtcgtcgaag gcgtgccaca gggcaatcac
    65701 acccacatac aaaaacagca gtaccagttc agaagtcagg cgtgcatccc atacccacca
    65761 ggtgccccac atcggttttc cccatgcaga gccggtaacc agggcaataa aggtaaacac
    65821 ggcaccaatg ggggccatcg ccgccaccgc caggttggcc attttcatct gccagacaag
    65881 gccaataaac gccgccaccg ccattgatgc ataaatgccc atcgaccaga tcgccgcagg
    65941 cacatgcagg tagataatgc ggtagctatt tccctgctga taatcagccg gagcaaaacc
    66001 gaatccccag atccagccga cggtaagcac gaccacactg gcaattgcca gccacggtat
    66061 aaaccagcca cagatttgat acagccgtgg tgggatcgcc agttgatgca gtgttttcca
    66121 catagtttcg ataccagact cgaacaaaaa tcagtaatcc agcgttattg aatgctgatt
    66181 cgtaacgctg ccgccgtcgc aaaaggactt aatgtcgcgg tgcctgccag caacgcgcct
    66241 aaaattgcca gatacccatc aacgggcaaa tgcatagaag ccgcgtccat cgcggcggtg
    66301 gcaaagatga gtaatgggat agtcagcggt aacaccagta tgctgagcag cacaccaccg
    66361 cgcttaagtc ccactgtcag cgccacgccc ggcgcaccaa gaaagccaag cgtaggggtt
    66421 cccagcagta gcgttaacgc catcacttgc cagccataaa catccattcc cagtagcatt
    66481 gctaccaggg gcgaaaggat gagtaacggc agaccggtta ccatccagtg cgccatcacc
    66541 ttcgccagca caacggcggg caagggtaac ggcaacaaca tcaattgttc aagactgccg
    66601 tcctgcaaat cgtcacggaa cagtcgctcc agtgccagca aggatgaaag cagcgcagca
    66661 acccagataa tgcccggcgc aatacgcgcc agcagttgcg gctccggacc gatactgagc
    66721 ggaaaaaggg taatgacaat caggaagaac cacagcgggt tggcgatttc ggcgctatgg
    66781 cgaaacgcta cgcgcagctc aagacggaaa atgcgccaga acattattcg cccctcgttt
    66841 gcgtcagtga aatgcggcga attttatttt cagcaacgtt gagcggctgg tgggtagtca
    66901 gaatcacaat ccccccctgc tccgtatgct gcgccatacg ctgggtcaga cgatcgacgc
    66961 cgttaacgtc aatcgcggta aaaggctcgt cgaggatcca taacgtggca cgggtcagcc
    67021 acagacgcgc taaagcgacg cggcgttgtt gcccggcaga aagttgattt accggaatat
    67081 cttcgaatcc ggcaagcccg gcctgcgcca gggcttccag acattgtgcg gtatcgccat
    67141 cgcgatgata aaagtgcaga ttttctaacg ccgtcagccg ggttttgatc cccggctgat
    67201 gccctatcca taacaggttt tgatggtagc tgtcgcgtac ctgatgcaag ggctgccctt
    67261 gccagagaac gtcacccgca tcagggcgag acaaccccgt cagcaaacgg agaagcgttg
    67321 tcttccccgc gccgttgcta ccggtgattt gtacccactc tcctgcgttc agcgtaaatg
    67381 acaagccact aaataaggtt cgttcatccc gctcgcaaag taactctctg gcttcaagca
    67441 tacccacgca ataaccctgt taaaaacctg gctcgacttc acgcatatcg ggcagcttgt
    67501 gcgctatccc tttatggcaa tcaatacagg tttgcccatc tttcaccgcc tggtcatgca
    67561 tcttcgcggc aaccgatttc tgggcggttg tatccatata ctcgaagttg tgacagttac
    67621 ggcactcctg cgagttattg tccttcatgc gccgccactc attctgtgcc atcgtcaggc
    67681 gatgagcttc aaatttctgc ggcgtgtcaa taacgccaaa aattttacca tacagctctt
    67741 tacttgcttt gagcttgcgt atcatcttcg gcacaaactc gtgcggaacg tgacaatccg
    67801 gacaggtcgc acgaacgccg ctacggttgt tgtagtgcac ggaatccatg tattcctgat
    67861 acaccgtgtt acgcatttcg tggcagctaa tgcagaactc ttcggtattg gctttttcca
    67921 tcccggtgtt aaagccgccc cagaagacga tgccgccaac aaaaccgatc aacagcagcg
    67981 tccccagcgc cagacggctg ggggtacgcc accatttcca caggcgctta atcagaccag
    68041 gcttacggtc agaatttccc ataataacct cttatttccc gtaacctttt gatggggtaa
    68101 aggtattccc cacgattggc gcggtatcgg cctgcggtac gtgacattgc agacagaaat
    68161 aacgacgcgg agccacttct gcgcccactt tgccgtcgct gtccataaag tgagtaggac
    68221 tgatacgcgg cgcgccagtg gtgcgatagc tttcgacacc gtggcactgc aagcagcggt
    68281 tggtattggt cgttacctga taaccttcaa cgctatgcgg gatcattggc ggctgattca
    68341 catagttcag cggcatccga tcctgctctt ttggcatccg aatggcccct tcctgcgtcc
    68401 cggatacttc cggcgactgg ctaaaatcga cgccattagc cgcccaaacc gcgccgctca
    68461 ccaccagggc cagcatcgcc gtccattgac acagcgcttt cttcaggtca tggcttttca
    68521 tgatttcgct cccgaactcc atcgtgtagt tattgtaaat acatcctcag aacagacatc
    68581 cacgcagcga ccgcacgtca tgcaatcgcg gctggtgacc tgcaccgggc tttgttcatc
    68641 cagcaccggg gcacgtagca catgcggttc cgggcaaaca tgaaaacaat ccatacagcg
    68701 gttacatttc tgccgatcgg tggccgcaac ggtaatcaca cctttgctac ccagcacgcc
    68761 atacagcgcg ccaaccggac aaatgtgccc gcaccagccg tgttcaacga ccagtagatc
    68821 aaataaaaac agcgcgagaa taagcagcgc gccgctgccg aatcccatta ccagactgcg
    68881 ccccatcagg gaaacggggt tgatccattc ccagatgagc gtgccggtta aggccgatcc
    68941 cagcagaatg accaccaaca gcacgtagcg aatgtggcgt gggatcgtcg cagactgatt
    69001 gaggtcaaag cgcctgcgta accagttcgc taagtcggta atcgggttca gcgggcagac
    69061 ccagctgcaa aataatcgct ttcctgccag ggcgtagagc acggtgataa tcaccgctcc
    69121 ggtcagcgcc accgaggccg gcagatgacc gctggcaaga ctttgcagcg tcatcagcgg
    69181 atcggtcagc ggaacggtgt cgaataacag gctactgctg tagttgccgt gcaagatcca
    69241 cacaccaaac cacggaccgc tcaaaaacat ccccagcacg aagaactgac aaaggcgacg
    69301 taacactaac caacggtgac tgcgccacca gccttttttc tccagcgcct cgcgcccggc
    69361 gtcacgttta cgatttgcca ttgttcccct ccagccagcc gaagcggtaa tggtgaccta
    69421 actccccttt cgctagtgac agcggtaaca ccttgattgc cggttgttcc agcacgcaca
    69481 ctttttcgca cttaccgcaa ccggtacagg cgtcgctgtg aaccgtcggc agaaagcggg
    69541 cgtgcttacc ggtacgcgtg ttgcgctcca gctccagggt gatggcctca tcaattttcg
    69601 ggcattcgcg ataacaaaca tcgcagcgca gcccctgaaa gttgagacag ttttcctggt
    69661 ccaccagtac cgccagcccc attcgcgcgt cgtcgatcga ttcgatctca cgatccagcg
    69721 caccgctcgg gcacactttg gcgcacggaa tgtcctcaca catttcgcaa ggaatatccc
    69781 gtgcgacaaa atatggcgtg cccgccgaca ggccagaggc cagcgtcgcc agttttaagg
    69841 tgtcgtaagg gcaagcctga acacactgac cgcaacgcac acaggcactg gcaaaggcgt
    69901 tctcgtttat ggctccgggc gggcgcaacc gcacgccaga tgcgcgtgcg gtttgctgtt
    69961 gtaaccccag cgccacaccc acggcagcca gcccgccagc tgtgcgaacg acatcgcgca
    70021 gaaagcggcg gcgaccattt tgaggtttcg ctgaccggga cataatggct tacaccttct
    70081 ccagtttgac cgcgcacttc ttgaagtccg tctctttcga gagcggatcg gtcgcatcca
    70141 gcgtcagttt gttaaccagc tgtgcggcat cgaagaacgg catgtacacc aggccctgcg
    70201 gcggacggtt acgaccgcgc gtttcgacaa tcgagatcac ttcgccacgg cgagaaacca
    70261 ctttcacttt gtcgccacgg cgcagatcgc gcgctttcgc atccagcggg tgaataaaca
    70321 ggaccgcttc cgggaaggcg cggtgcagtt ccggtacacg gcgagtcata ctgccggtat
    70381 gccagtgctc caggacgcgt ccggtagaga gccacaggtc gtactcttca tccggtgctt
    70441 ccgccgcagg ttcgaacggc agcgcgaaga tcaccgcttt gccatccggt ttaccgtaga
    70501 acttgtagcc ttcgcccgct ttcacgtacg ggtcgttacc ttcgctgtaa cgccactgcg
    70561 tttctttacc gttcaccacc ggccagcgca gaccgcgtgc tttgtggtag tcatcgaacg
    70621 gcgcgagatc gtgaccgtga ccgcgaccaa accatgcgta ctcttcgaac agcccttttt
    70681 gcagatagaa gcccagctcg cgggattcat cgttcagctg atcttccgcc agttcggata
    70741 gcgggaattt gctcacttcc ggggtggcat acagaacttc gtacagcgtt ttgccacgca
    70801 gttccggttt cttcgccagc agctcttccg gccatacttc ttcagttttg aagcggcggg
    70861 agaactggac taactgccag agatccgatt tcgcttcgcc cggtgcctgt acctgctgac
    70921 gccagaattg agtacggcgt tcggcgttac cgtaagcccc ctctttctct acccacattg
    70981 cggtcggcag gatcaagtcc gctgccagcg cactgactgt cggatacgga tcggaaacga
    71041 tgataaagtt gcgcggatcg cgccagcctg gcatacgctc ttcattaatg ttcggcccgg
    71101 cctgcatgtt gttggtacac atggtccagt aaacattcag cttgccgtct ttcagcgcac
    71161 ggtcttgtgc caccgcgtgc agaccgattt tcgccggaat ggtgccactc gggatattcc
    71221 acttcttctc gcaaatatca cgatgtttct cgttagtcac caccatgtcc gcaggcagac
    71281 ggtgagcaaa ggtgcccact tcacgcgcag taccacacgc ggaaggctgc ccggtcaggg
    71341 agaacggacc gcaacccggc tgggaaattt tgccggtcag caggtgcagg ttgtagacca
    71401 ggttgttggc ccacacacca cgggtatgct ggttgaagcc catcgtccag taggagatga
    71461 ctttcttgtt cggatcggca tacagctgcg ccagttgttc taactggtct ttcggcacgc
    71521 cggtcatttc ggcggttttt tccagcgtat attcagcaac gaaggctttg taatcttcaa
    71581 agctcatcgg ttcggaggcg tcagaacccg gattcttcgc tgctttttcc agcggatggg
    71641 tcggacgtaa accgtagccg atgtccgtcg cccctttgcg caggttaacg tgtttgctga
    71701 agaagtcctg atttatcgca ttgttttgaa tgatatagtt ggcgatatag ttcaggatca
    71761 ccaggtcaga ttgcggcgta aagatgatgc cgttatccgc cagctcgaag ctacgatgct
    71821 ggtaggtaga aagcaccgcc acggtgacgt tctggttgga gagacgacgg ttagtgatgc
    71881 gcgaccagag gatcgggtgc atctccgcca tgttggagcc ccacagcaca aacgcgtcag
    71941 cctgctcgat gtcgtcatag cagcccatcg gctcatccat accaaaggta cgcataaagc
    72001 caactaccgc cgacgccata cagtgacgcg cgttcgggtc gatgttgttc gaacggaatc
    72061 ccgctttaaa cagcttggac gcggcataac cttcccagat agtccactga ccagaaccga
    72121 acataccgat cgattccggc cctttttctt tcagggcggt tttgaatttc tcttccatca
    72181 catcgaaggc ttgatcccag gtgattgggg taaattcgcc ttctttgtca tatttaccgt
    72241 ttttcatacg cagcagcggc tgcgtcaaac ggtctttacc gtacatgatt ttgggcagga
    72301 aatagccctt aatgcagttc agaccacggt taaccggtgc gtccgggtcg ccctgacagg
    72361 ccaccacacg cccctgctgc gttccgacca gaacgccgca acccgtaccg cagaaacggc
    72421 acggcgcttt atcccattta atggcttcct gctgaccaac aacggcgcgg gcaacgcccg
    72481 gcacgctgag accggcagcc gccgcagcgg ccgcaacggc gttagctttc ataaagctac
    72541 gacgactgag tttcatggtg tttcctcacc ttgctcttcc tgctggtgat aaaccagcga
    72601 caccgccagc acgccctcta cgttgcgtac tgactcaatg gtttggatca gcgtttcgct
    72661 gtcttctgct tccaccacca caatcaactg gccgctcggc gcgtcgctga cagcaacttc
    72721 acagccggga aaggcgttca gttgggtgct gatgtctgaa attcgttcgc ttttggcctg
    72781 cacgaccagg ctgcaaactt gccagttagt gtacatggag atactccgca gttatggctg
    72841 ataccggaca gctggctgca catgctccac atgcgttgca aagttggcta ttaagttgcg
    72901 gctggtagat cccggagagc gtcgggcgaa agataattgc cattggctca cagctatcct
    72961 gacagcggcg gcattcaact gactgatacg ccaggcacgc ctccccgatg gtgaacagca
    73021 aatcccaggc cctggtgtgg cgcggagaaa atagcgattc ggggcaagcc tgcgcgcagg
    73081 cgtaacagaa gctgcactcg ttatttttga aattaacgct cggatagcca cccgcgccgc
    73141 gttgcagaat gttgttttca caagcattaa tacaggcgtc gcagcgagtg caatgggtca
    73201 gaaaatgaga ttcatcaccg gaccagggcg gacggatacc gttactggct ttgcgccagc
    73261 gaccagtgag tatgccccga cgggatgcat caatcttcac attgaccttc catcattaac
    73321 gcgctctgaa aattgagagc gaccaaataa accgcataat taataagcca tttttatagc
    73381 cgctaagata ttaaaggatg tgtcaaagat gcataccccg atcggggtaa aacctgtagc
    73441 aggatcaaaa gagtggcagg aagcgtggca aaaacgggct tttgctcaca tttcaaatgg
    73501 ttataaacat atttatatag cgattaattc gccagagata tttctgctgg tttgttttct
    73561 catcagaatt taacactaaa agagcaggta aaattatctg aatgttcttt aagttattca
    73621 taaagcaaat taataaatct gatgaatatg ttaaccttca gcgacatcat cggtgaaaac
    73681 ctataaatga agaaggaaag caaaaaaatg aagaccattc tacctgcagt attgtttgcc
    73741 gctttcgcca ccacttccgc ctgggcggca gaaagcgtcc agccactgga aaaaatcgca
    73801 ccttatccac aagctgaaaa aggcatgaag cgccaggtga ttcagttaac cccgcaagaa
    73861 gatgaatcta ccctgaaagt agaactgtta atcggccaga cgctggaagt cgattgcaat
    73921 ttgcatcgtc tcggcgggaa gctggaaagc aaaacgctgg aaggctgggg ctatgattat
    73981 tatgtctttg ataaagtcag ttccccggtt tcgacgatga tggcctgccc ggatggcaag
    74041 aaagagaaga aatttgtcac cgcgtatctg ggcgatgccg gaatgctgcg ttataacagc
    74101 aagctgccga tcgtggtgta tacgccagac aatgtagatg tgaagtaccg cgtctggaag
    74161 gcggaagaga aaattgacaa cgcggtagtt cgctaaactg ctgtgaagtg ctgcaaccgc
    74221 caggtcggat aaggcggtta cgccgcatcc gacatctaac gcccgagccg gttgcctgat
    74281 gcgacgctga cgcgtcttat ctgcatacgc cacatccggc ataccatgcc ggatgtggca
    74341 catcatcaca acgcaatatc cgccacttct ttttgaacgg gttgcggttt caactgcggt
    74401 ttcggcgtac tatctgctgc ctgcggtttg tcgtatttca gccccagcac ttcgctggtg
    74461 tactgcaact cgcgttctgt tgccgctaca tcaccgttca gcttgcgtcc ataagacgga
    74521 acgatcgctt tcaacgtagc ctgccattgc gggctggaaa cgcgatcgcc aaatactttt
    74581 tccagcagat ccaacataat cggcgcggcg gttgacgccc ctggcgatgc ccccagcagc
    74641 gcggcaatgg ttccttgttg gtcgctgacc acttcggtac ccagacgcag tacgccacct
    74701 ttctcggcat cacgcttgat aatctgcacg cgctgccccg cttgccacaa acgccagtcc
    74761 tcttttttcg cctgcggata gtactctttc aacgcttcaa aacgatcctc ttcactcaac
    74821 atcacctgac tcaccagata tttcaccaga tcgaaattat ccagcccgac gtgcatcatc
    74881 ggcatcacgt tagaggtggt ggtggaactc atcagatccc acaatgaacc gtttttgagg
    74941 aatttggttg agaaggtggc aaatggccca aacagcacga cgcgtttacc gtccagaaca
    75001 cgggtatcga tatgcggaac cgacatcggt ggtgcgccaa cggatgcttt accgtaaacc
    75061 ttcgccagat ggtgattaac cacgtccggg ttttccgaaa caaggaactg tccgcccacc
    75121 gggaaaccgg cgtagtcttt cgcttccgga atccccgatt cctgtaacag cttcagcgcc
    75181 gcaccgcccg cgccgataaa gacaaatttg gcgcggatgt tctgtgcagt gccatttttc
    75241 agatcggcaa cagtgacggt ccaggtgtta tcgtcattac gctttagggc gcggacttcg
    75301 ctgctgagtt gcagcgaaaa attagatttc ttctgcaagg aagcaattaa ctggcgggtg
    75361 atctcgccat agttcacatc agtaccaatt tccgtacgcg tggctgccac tttctgttgc
    75421 ggatcgcgcc cttccatcac taacggtgcc cactctttga tctgcgcgtg atcttcagag
    75481 taacgcatac cgcgaaacag cgagctttgt tgcaacgcgg cgtaacgggc gcgcaggaaa
    75541 ttgacgttat cctcgcccca gacaaagctc atatgcggaa cggtattgat aaatgaacgc
    75601 ggagtacgca gcacgccgcg ctcgacctgg tgcgcccaga actggcgcga aatctgaaat
    75661 gcttcgttaa tggcgactgc tttttcaata ctgatgctgc catcggcgtt ttgcggagtg
    75721 taattgagtt ccatcagtgc agaatgcccg gttccggcgt tgttccagcc gttcgaactc
    75781 tcctgcgcga caccctccag gcgctccacc atggtcatcg accattcagg ctccagctcg
    75841 cgtaaatagg tccccaacgt ggcgctcata atgccgccgc caatcaacag tacatcagtt
    75901 tcctgctcct cggatgcttt cgcttttgcc gccatcgaaa cggcattaag ccccacggcc
    75961 atcgagaaga gcatggcagt cacttttttc atcttgttaa tgccttactt ttagtcgctt
    76021 tattgcaggt gagatttgcg cggcatcaac ggtaacactt aagtaac
//
LOCUS       DAZGQL010000010        71847 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.010, whole genome shotgun sequence.
ACCESSION   DAZGQL010000010 DAZGQL010000000
VERSION     DAZGQL010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 71847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 71847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..71847
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.010"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            48..440
                     /gene="ydeI"
                     /locus_tag="ACHKIR_000793"
     CDS             48..440
                     /gene="ydeI"
                     /locus_tag="ACHKIR_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HFO9988579.1"
                     /translation="MKFQAIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISHKGEDRYVFRDKSGEINVVIPAAVFDGREV
                     QPDQMINISGSLDKKSAPAVVRVTHLQK"
     gene            695..1585
                     /gene="dgcZ"
                     /locus_tag="ACHKIR_000794"
     CDS             695..1585
                     /gene="dgcZ"
                     /locus_tag="ACHKIR_000794"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HFO9988580.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYELCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGLLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNAEPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAAN
                     DEEACRAGVRICQLVDNHAITHSEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
     gene            complement(1700..1804)
                     /gene="mgtT"
                     /locus_tag="ACHKIR_000795"
     CDS             complement(1700..1804)
                     /gene="mgtT"
                     /locus_tag="ACHKIR_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtT"
                     /protein_id="HFO9988581.1"
                     /translation="MNGDNPSPNRPLVTTVYKGPDFYDGEKKPPVNRR"
     gene            complement(1804..1899)
                     /gene="mgtS"
                     /locus_tag="ACHKIR_000796"
     CDS             complement(1804..1899)
                     /gene="mgtS"
                     /locus_tag="ACHKIR_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HFO9988582.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
     gene            complement(2026..3213)
                     /gene="ydeE"
                     /locus_tag="ACHKIR_000797"
     CDS             complement(2026..3213)
                     /gene="ydeE"
                     /locus_tag="ACHKIR_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HFO9988583.1"
                     /translation="MNLSLRRSTSALLASSLLLTIGRGATLPFMTIYLSRQYNLSVDL
                     IGYAMTIALTIGVVFSLGFGILADKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLF
                     FALINCAYSVFATVLKAWFADNLSSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSI
                     NLPFWLAAICSAFPMLFIQIWVKRNEKIIATETGSAWSPKVLLQDKALLWFTCSGFLA
                     SFVSGAFASCISQYVMVIADGDFAEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRAL
                     MTAGTLCFVIGLVGFIFSGNSLLLWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKAS
                     YFSAQSLGWLGAAINPLVSGIVLTSLPPFSLFIILALVIIAAWVLMLKGIRARPWGQP
                     ALC"
     gene            3408..4307
                     /gene="eamA"
                     /locus_tag="ACHKIR_000798"
     CDS             3408..4307
                     /gene="eamA"
                     /locus_tag="ACHKIR_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HFO9988584.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVASLILDGSATMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFFGAV
                     LIMTGLYINVFGLRWRKAVKVGS"
     gene            complement(4338..4556)
                     /gene="marB"
                     /locus_tag="ACHKIR_000799"
     CDS             complement(4338..4556)
                     /gene="marB"
                     /locus_tag="ACHKIR_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HFO9988585.1"
                     /translation="MKPLLSAIATALILFSAQGVAEQTTQPVVTSCGNVVVVPTSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(4588..4971)
                     /gene="marA"
                     /locus_tag="ACHKIR_000800"
     CDS             complement(4588..4971)
                     /gene="marA"
                     /locus_tag="ACHKIR_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HFO9988586.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNN"
     gene            complement(4992..5426)
                     /gene="marR"
                     /locus_tag="ACHKIR_000801"
     CDS             complement(4992..5426)
                     /gene="marR"
                     /locus_tag="ACHKIR_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HFO9988587.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            5638..6303
                     /gene="marC"
                     /locus_tag="ACHKIR_000802"
     CDS             5638..6303
                     /gene="marC"
                     /locus_tag="ACHKIR_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HFO9988588.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            complement(6328..7518)
                     /gene="ydeA"
                     /locus_tag="ACHKIR_000803"
     CDS             complement(6328..7518)
                     /gene="ydeA"
                     /locus_tag="ACHKIR_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HFO9988589.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGELITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(7660..7779)
                     /gene="yneK"
                     /locus_tag="ACHKIR_000804"
     CDS             complement(7660..7779)
                     /gene="yneK"
                     /locus_tag="ACHKIR_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HFO9988590.1"
                     /translation="MVTPVSISNFISLPDDFPVRNIAPQVKEEQAPFLIVIKM"
     gene            complement(7863..8738)
                     /gene="yneJ"
                     /locus_tag="ACHKIR_000805"
     CDS             complement(7863..8738)
                     /gene="yneJ"
                     /locus_tag="ACHKIR_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO9988591.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAATKVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPAMLAEFNRRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVTRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDVSDSAKQS"
     gene            8839..10227
                     /gene="sad"
                     /locus_tag="ACHKIR_000806"
     CDS             8839..10227
                     /gene="sad"
                     /locus_tag="ACHKIR_000806"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004030 - aldehyde dehydrogenase [NAD(P)+]
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HFO9988592.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGADDIENALQLAAAGFRD
                     WRETNIDYRAEKLRDIGKALRVRSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNNGVSQMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEENALGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITIAKDAEHALELANDSEFG
                     LSATIFTTDETRARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            10291..11217
                     /gene="glsB"
                     /locus_tag="ACHKIR_000807"
     CDS             10291..11217
                     /gene="glsB"
                     /locus_tag="ACHKIR_000807"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HFO9988593.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRMGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCIELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            11217..11576
                     /gene="yneG"
                     /locus_tag="ACHKIR_000808"
     CDS             11217..11576
                     /gene="yneG"
                     /locus_tag="ACHKIR_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HFO9988594.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGVKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGEALSEEQQR
                     YIVTVIYHWLVIQMNQP"
     gene            11715..13133
                     /locus_tag="ACHKIR_000809"
     CDS             11715..13133
                     /locus_tag="ACHKIR_000809"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFO9988595.1"
                     /translation="MHVQPISTFRLFQEGHLLRNSIAIFVLTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLAINFSNMMFI
                     VTVALLVARDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPQMFSARLGIA
                     TMAICPIMVSFSVAAINSLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFAQHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGCASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            13361..14812
                     /gene="uxaB"
                     /locus_tag="ACHKIR_000810"
     CDS             13361..14812
                     /gene="uxaB"
                     /locus_tag="ACHKIR_000810"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFO9988596.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTGLVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILKKGMREAVRPLC"
     gene            15009..15923
                     /gene="yneE"
                     /locus_tag="ACHKIR_000811"
     CDS             15009..15923
                     /gene="yneE"
                     /locus_tag="ACHKIR_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HFO9988597.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTEDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISVVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
     gene            complement(15927..16685)
                     /gene="tam"
                     /locus_tag="ACHKIR_000812"
     CDS             complement(15927..16685)
                     /gene="tam"
                     /locus_tag="ACHKIR_000812"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030798 - trans-aconitate
                     2-methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HFO9988598.1"
                     /translation="MSDWNPSLYLHFAAERSRPAVELLARVPLENIKYVADLGCGPGN
                     STTLLHQRWPAARITGIDSSPAMIAEARSTLPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNSHGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQSIIDWVTATGLRPWLQDLTESEQQHFL
                     TRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(16742..17032)
                     /gene="lsrG"
                     /locus_tag="ACHKIR_000813"
     CDS             complement(16742..17032)
                     /gene="lsrG"
                     /locus_tag="ACHKIR_000813"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HFO9988599.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDAVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            complement(17056..17931)
                     /gene="lsrF"
                     /locus_tag="ACHKIR_000814"
     CDS             complement(17056..17931)
                     /gene="lsrF"
                     /locus_tag="ACHKIR_000814"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HFO9988600.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFTLKGCGALDWGMQSRLSRIF
                     NPKTGKTVMLAFDHGYFQGPTTGLERIDINIAPLFEHADVLMCTRGILRSVVPPATNK
                     PVVLRASGANSILAELSNEAVALSMDDAVRLNSCAVAAQVYIGSEYEHQSIKNIIQLV
                     DAGMKAGIPTMAVTGVGKDMVRDQRYFSLATRIAAEMGAQIIKTYYVDKGFERIVAGC
                     PVPIVIAGGKKLPEREALEMCWQAIDQGASGVDMGRNIFQSDHPVAMMKAVQAVVHHN
                     ETADRAYELYLSEKQ"
     gene            complement(17958..18980)
                     /gene="lsrB"
                     /locus_tag="ACHKIR_000815"
     CDS             complement(17958..18980)
                     /gene="lsrB"
                     /locus_tag="ACHKIR_000815"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
                     /protein_id="HFO9988601.1"
                     /translation="MTLHRFKKIALLSALGIAVISMNVQAAERIAFIPKLVGVGFFTS
                     GGNGAQQAGKELGVDVTYDGPTEPSVSGQVQLINNFVNQGYNAIIVSAVSPDGLCPAL
                     KRAMQRGVRVLTWDSDTKPECRSYYINQGTPAQLGGMLVDMAARQVNKDKAKVAFFYS
                     SPTVTDQNQWVKEAKAKIAKEHPGWEIVTTQFGYNDATKSLQTAEGILKAYSDLDAII
                     APDANALPAAAQAAENLKNDKVAIVGFSTPNVMRPYVERGTVKEFGLWDVVQQGKISV
                     YVADALLKKGSMKTGDKLDIQGVGQVEVSPNSVQGYDYEADGNGIVLLPERVIFNKEN
                     IGKYDF"
     gene            complement(18992..19984)
                     /gene="lsrD"
                     /locus_tag="ACHKIR_000816"
     CDS             complement(18992..19984)
                     /gene="lsrD"
                     /locus_tag="ACHKIR_000816"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HFO9988602.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVAVYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            complement(19984..21012)
                     /gene="lsrC"
                     /locus_tag="ACHKIR_000817"
     CDS             complement(19984..21012)
                     /gene="lsrC"
                     /locus_tag="ACHKIR_000817"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HFO9988603.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGVLVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLFGVS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYVTGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFIPNQTGTVLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGMVLLAVLVFDGRLRCALERNLRRQKYARFMMPPPSVKPAS
                     SGKKREAA"
     gene            complement(21006..22541)
                     /gene="lsrA"
                     /locus_tag="ACHKIR_000818"
     CDS             complement(21006..22541)
                     /gene="lsrA"
                     /locus_tag="ACHKIR_000818"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HFO9988604.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYTRLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGLAKKQLSIQKMNNLLVALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFRRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPALREKSLSASQKLWLELPGNRPQHAAGTPVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNGKEINRLSTGD
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGGNQQKILIAKCLEASPQVLIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLLISSDLEEIELMADRVYVMHQGEIAHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            22790..23743
                     /gene="lsrR"
                     /locus_tag="ACHKIR_000819"
     CDS             22790..23743
                     /gene="lsrR"
                     /locus_tag="ACHKIR_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HFO9988605.1"
                     /translation="MTINDSVISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            23822..24847
                     /gene="lsrK"
                     /locus_tag="ACHKIR_000820"
                     /pseudo
     CDS             23822..24847
                     /gene="lsrK"
                     /locus_tag="ACHKIR_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416028.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0071518 - autoinducer-2 kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer-2 kinase"
     gene            complement(24913..27951)
                     /locus_tag="ACHKIR_000821"
     CDS             complement(24913..27951)
                     /locus_tag="ACHKIR_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001175011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HFO9988606.1"
                     /translation="MQKYKKRISILTKSEINELYQVPSFNPVERDEFFSLDISLKREI
                     DKIINIESRVYLILITGYFRYKPVIPEFTLKDVKRDVKYIIQTYYKNESTNLNLNIPK
                     STKYRLVKRMLSTLGFEQLTPSIKNELSERLNDVATICMEPKYIFDELLAFLGQRRIA
                     LPGYSTVQNLVSDALNFERKRTINILSKRIYPATAQKITSIIHDDGILNSIRGYNYST
                     RDFSASEIENELRTHNVISSIYYEIKDIINSLGISQGNMEYYATIVKHSTTFRLKRHP
                     KWQGILYLCCYLYFRYREINDKIITAFRYLVKKHKDASVLFAKARATEEIDLINKKLK
                     FASKIFNFFVDSNIEDSIPFGYVREKVFSLISKDDMHLVSEFFDKNKIDVIEYQWQYI
                     DSHHLKIANSIRKLFLAIDIVYDNEKSVIGQQITAAKDDLRGKGKLDAKDLRVIHPSE
                     KNYIVKEGEINTQRFEFHMYSRVSNHLENGVFYAAESEQNKRLEDDLIERSYWHKNKS
                     ILIEKTGLERLITPITQTLNELENTFSDKLARITIGINSDANEFVKKQPHSNRLQWSL
                     ASKKWKTSIDNPIYNQIKNIGIVDVMKYVNNETNFLSVFNAISSRKNNLEADYNDLLA
                     CIFGNGANYGVHRMASASDRSIGILRDVNDKFIRPETTGMANTVISDAIANLSVYKYW
                     TINEDSPFGSVDGQKHTCRINTFKARYSAKYFRKGKGVSAMTLVSNHVPLSTVVISPN
                     EYEGHFAFDLLYNNTSEIQPKSLATDNHGINNVNFAILDIFGYQFSPRYAKFKKIFNE
                     LFDVILESDKLIIKLKKQFNFKLIEHEWENIQHIICSLSRKTTIQSTVVKKLSNTKRS
                     NKTLAALREYDRVIKCIYVLDYADNKTLRQFVQQALNRGEAYHQLRRAIASVNGNQFR
                     GGNDYQIDQWNDCARLIANCVIYYNSAILSRLVEKFEKENNKKAIDVLANLSPVAWRH
                     ILLGGNYSFEDQVAITNLDSLLEGVDPLSDIGDIESEYDG"
     gene            28108..28689
                     /locus_tag="ACHKIR_000822"
     CDS             28108..28689
                     /locus_tag="ACHKIR_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO9988607.1"
                     /translation="MALLGYARVSTSHQKLTHQISELKNAGVREDRIFTDMMTGATDE
                     REGLQRLLARAEKDDVIICTKMDRLGRNTADMIRVVDSCYKKGIAIRFLENGLSTEGT
                     MGKMVIQILAAVAEAERERILERTNDGRIAALAAGVKFGRKKHPRTPTALELISQGES
                     LGSVTEKTGISRSTYFRLKRTIKNDAKIATFYK"
     gene            28747..29883
                     /gene="pilO2"
                     /locus_tag="ACHKIR_000823"
                     /pseudo
     CDS             28747..29883
                     /gene="pilO2"
                     /locus_tag="ACHKIR_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681793.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 4b pilus protein PilO2"
     gene            29873..30325
                     /gene="pilP"
                     /locus_tag="ACHKIR_000824"
     CDS             29873..30325
                     /gene="pilP"
                     /locus_tag="ACHKIR_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilP"
                     /protein_id="HFO9988608.1"
                     /translation="MPDKAKTLLLSALLLPATAMATTGNAEQQNTQQIRIEQIQEETA
                     LYEAMLAREKIRHELRTLEQGVPAEGGRQQESISAVTTNAQALPSPRLTAIFGVQPQL
                     TARIELPGGSISDVKAGQNIPGTTYHVEKITPDSVVLEQSGTRKTLRP"
     gene            30410..31918
                     /locus_tag="ACHKIR_000825"
     CDS             30410..31918
                     /locus_tag="ACHKIR_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HFO9988609.1"
                     /translation="MLDPADLPPDDILDYVAIDTDNTGQLRIRVVEGKKHLRAVQEYL
                     TRLRARYQGRVGDFEFTTLDVIARLRQDATTTGDENAINPIQQKMLGYIRHSASLGAS
                     DLHMTPGRDNTDFTYVEARVHGELEVLDILRKEEGLELLGATYSGMTDVIKGTQFDPG
                     VPQDARLSEQYLKLAGLFGARYSHYPCVGGLYAVLRLIKDDSQQIPTFSMLGYHPDQE
                     CTLRRILQRPEGIITLSGPTGSGKSTTLRTASAAYLEQYGFNNTGGILLPRRRLFTIE
                     SPPEGRIPGAIQTAVMDTTRGWVDSIKSALRLDPDSILNGEIRDHDSAITAIKAAMTG
                     HLMLTTIHANDPINILERLEMEGVQARMIADPQLFIGLLSQRLVQLICPHCRHPWNDV
                     ASRRTEDERHLVETFCNADAVYLRNHEGCPHCWRGVTGRTVIAEVISPDARFFQLYRE
                     KGRIEAKTYWHRELGGMTRNQHLLGKINSGLVDPLAAHYISPIDEDSYTLLR"
     gene            31920..33020
                     /locus_tag="ACHKIR_000826"
     CDS             31920..33020
                     /locus_tag="ACHKIR_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000670135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HFO9988610.1"
                     /translation="MKFLEKLNSTELDLSALNSWTARKTFGLKRAEFYESLGGMIRDG
                     TPSLRALEFICNVETDFGTRKGTSGMYFLARECIASLKASGMLSGALPTWVPPEEAAL
                     IRNGEERGNIAEALFQVAKTVKARREMVSSLITLCLYPLLLLSLCVVNMYNAHTRIIP
                     IVSAFAPEEKWSFQMKVLAAMSELVIGYGLWLSGAIITLVIIIRFSLSRYTGPGRRLL
                     DKLPPWSLYQTFHGVNYLFSISSMLQINIPLSQALLRIEKSAEHNKWLKERIQAIRKN
                     VLSGQNLATAMRNSDYDFPSKICINKLLLNSEREDSVSAMNTYAGQWLEDAKKSVRKS
                     GLIITGISGGFVFFFILNMISAIYSISDMLKQ"
     gene            33094..33630
                     /locus_tag="ACHKIR_000827"
     CDS             33094..33630
                     /locus_tag="ACHKIR_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009447271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4 pilus major pilin"
                     /protein_id="HFO9988611.1"
                     /translation="MNFRARLSSYFSLKKDKEDDKGLTLLEIAGAMIIIAIVVALAMS
                     QMSSTMASSDNQRELKNLQTIATKMKQYKFQGRYTGTDYIKTLTESGALPADMIAGGN
                     KAKNAWGGDVTIAATADKYGYIITSNNVPKTNCVELINSLRSSSIFTKIMNTAPNTVD
                     PVTVCSSDKNNIKLETNS"
     gene            33684..34157
                     /locus_tag="ACHKIR_000828"
     CDS             33684..34157
                     /locus_tag="ACHKIR_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFO9988612.1"
                     /translation="MKWFYLSSLFLFCLPATAAVNSNTLDSCFIQAGKRYSIDPGLLK
                     VIARKESSLNPRAINHNRNASGKIISTDYGLMQINSTHIPGLKRMGIIRSENELLSNV
                     CLNIQTGAWILARHFQQCGVNWECLGSYNAGFNKNNTHRRMKYARLIYAAYMQGR"
     gene            34176..34811
                     /locus_tag="ACHKIR_000829"
     CDS             34176..34811
                     /locus_tag="ACHKIR_000829"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HFO9988613.1"
                     /translation="MFLIILLVISATLTIQLLAIRLAGHYLMQVHTLRRPRHLSLLCA
                     VLALTATITHIIRGTDITGLCFLLLMAGCMTLMTLTDAASCRLPRPFTLTFLMLGVAF
                     RLWQGDAAIALACALFWFLLLSLLRRYTSYRKGVETLGLGDVFLIAGVAMWSSANSTP
                     WIIGIAAASTMICMSLFPRSNTGAAIPFGPFICASLYIFTFFPGAIFHTGG"
     gene            34816..36180
                     /gene="pilV"
                     /locus_tag="ACHKIR_000830"
     CDS             34816..36180
                     /gene="pilV"
                     /locus_tag="ACHKIR_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001168069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shufflon system plasmid conjugative transfer
                     pilus tip adhesin PilV"
                     /protein_id="HFO9988614.1"
                     /translation="MQKDDDKGFVLFEILAGLIVIGIATPMIYSEIENWLNEQLYQSA
                     AYHADAYNTAARNYIADNNARLHSGSLPANFTADDLIRQGYLKQGFNHSPFGQSYITG
                     IRRNQTTGRLEALTCSTGGQTIKEEGLRSVAGQLPGLGGFISKNGTATGAFGAWTDKP
                     GDYGLTCSTGHIAVVMSGDDLQESDRLYRFQVAGRPELNQMHTAINMGGNNINNTGNI
                     NGQSATLKGDITSEDGWLITRNNKGWMNITHGGGFTMTDSQWIRAVNGKGITTTGEIK
                     GGKVSGGTVRSDGRLSTGEYLQLDKTATAGTKCSPDGLVGRTSTGAILSCQSGMWAGF
                     ESYPVGSPIPWPSATPPQGYLVMNGQSFSCSRYPQLARAYPGCKLPDLRGVFIRGWDN
                     GRGLDGDRNRHLLSYQADQSGVYHERGGWLKGHHSGMPYWAQGSTTEMRPKNIAFNYI
                     VKAS"
     gene            36795..37961
                     /locus_tag="ACHKIR_000831"
     CDS             36795..37961
                     /locus_tag="ACHKIR_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000486835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFO9988615.1"
                     /translation="MFQKKTRKMSLSRALDKYLKTVSVHKKGHLQEFYRVNVIKRHPM
                     ADRYMDEITTIDIAGYRDQRLAQINPRTGRQITGNTVRLELALLSSLFNIARVEWGTC
                     RMNPVELVRKPKISNGRDRRLTSGEERRLSRYFRDKNPQLYVIFHLALETAMRQGEIL
                     SLRWEHLDLQHGVAHLPETKNGSPRDVPLSRKARNYLQILPQQINGNVFSYTSSGFKS
                     AWRTALLDLKIENLHFHDLRHEAISRLFELGTLNVMEIAAISGHRSLNMLKRYTHLRA
                     YHLVSKLDARRKQTSKISPYFVPYPATVRNRNGVFIVTLHDFDLESRAETRELAISHA
                     SVLLLRALAQAAQRGERVPTPGELPANIDERVMICPLTNSQFMTKANSIFISSS"
     gene            38003..38293
                     /locus_tag="ACHKIR_000832"
     CDS             38003..38293
                     /locus_tag="ACHKIR_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9988616.1"
                     /translation="MKHLSSRAATADNNRIVTIEDVYTIAFSQPIHTESALGQRWMLI
                     HTGEYESASDAKRFLDTLVTGHCACMVTPSPVLDHDTWLRIRLLMGEIRDAK"
     gene            complement(38385..38705)
                     /locus_tag="ACHKIR_000833"
     CDS             complement(38385..38705)
                     /locus_tag="ACHKIR_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9988617.1"
                     /translation="MKIKQHKEKLEIIRDIIRETLLGNAALIVLAAMVPVVLVKPWDS
                     MTFIFMGAVCVSFAVILFWLVYIVCLYIGTLVKNIFIRIFAFSIWALSAEGAIIYNMI
                     KILS"
     gene            38965..39786
                     /locus_tag="ACHKIR_000834"
     CDS             38965..39786
                     /locus_tag="ACHKIR_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HFO9988618.1"
                     /translation="MKPNSTGIAARMMLSIDRARISEALLNNRQLQSTPLQIRYPQGV
                     REALGIMSEQLSLSVSDLTRILVEDALTEMFLPAENIVHRLICRTEHIMQAHDVCVTT
                     MTTLLAPWNIRPAVFRDPDKLADYLTGEILTALADWFYLNPEWLNGRVRYPLSRAGEW
                     PATQDAFCQLISASDNVDIIIWHSFPFAGVHSQEYCGVLFRKKKEINKAIIYPVLSLS
                     PVRMNKEKENWFHTARNLYAKTSVRTVTLRPAQAEYLMTGKILPAELFSIPLLPW"
     gene            39876..41078
                     /locus_tag="ACHKIR_000835"
     CDS             39876..41078
                     /locus_tag="ACHKIR_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001532112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /protein_id="HFO9988619.1"
                     /translation="MMMNMHITRTLIASAVLFSFNSTAATSYFEARNDAMGGTGVASS
                     HYGVAPLANPALLTKAGPEDDFSLLLPSVGGQLSDPGNISDNADKISDDWKAFDRAID
                     SQSNVSQTAARLKTRLQDFRHTHADAQVGTSAVATLPGDTLAAALMLKSYGTVSVDGK
                     VSDADLNYLESIANSNGQDVDKDRLTSQAFARAALVTDVGIALATELETAGQKWSLGF
                     TPKFQRVDLFNYNVLVKNYDSSAFKGDRYHNTRNGINADIGASMDLDDNWTLGLVAQN
                     LLPRSIETKEVNGVTETFRIRPQVTAGVSWHNDMFTTALDVDLTPASGFTSDSKRQFA
                     AIGAEFNAWKWAQLRAGYRQNLASNDGSAFTAGLGISPFDVVHIDVAGIAGTDNNYGA
                     VAQLQFTF"
     gene            41218..41388
                     /locus_tag="ACHKIR_000836"
     CDS             41218..41388
                     /locus_tag="ACHKIR_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9988620.1"
                     /translation="MFNKNKTHIENKAFMVEPFRDPTANECNVVIANDICIRVALSPS
                     EVFQPFSKNTNQ"
     gene            41437..41889
                     /locus_tag="ACHKIR_000837"
     CDS             41437..41889
                     /locus_tag="ACHKIR_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081199.1"
                     /note="Members of this family include DotD of type IVB
                     secretion systems and TraH of plasmid conjugative plasmid
                     systems, both lipoproteins; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotD/TraH family lipoprotein"
                     /protein_id="HFO9988621.1"
                     /translation="MNRMIPAGCLAVLCLNGCHLPPDNTAPTRKYGAASDVAVARHAQ
                     YQLWGQGIYSAALPERQSGGSLKAHSDRVSFDWEGDALELLNELARLRGQQFSYSGVR
                     LPLPVSLHVRDMTFADVLRLTEAQTAWRATLSQSAGRLHLSFMTPEKK"
     gene            41886..42722
                     /locus_tag="ACHKIR_000838"
     CDS             41886..42722
                     /locus_tag="ACHKIR_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HFO9988622.1"
                     /translation="MKTHLCLLLSALLLTGCAGQHHRSSAVMTENAPPPDIRAWLTPS
                     GNQDDGISDTRRQMLTDAGRTLGFRGGKAQRSWELIQALNARESTLNALYDFRPLISK
                     EGWLPPVIDEAQDVAHIRPDQIRTASRVWTILRPERFVSNPPGWRDWLLKGLSTTATP
                     GTEGRVVPEDRAQRRLWENALRQGWQEGRLNADLTLEANQKRLTRDYRGMMLYALLWR
                     QGMITRPDVTEQMQTVTGNGQKLITGDRVRRLKTHAEFTLQKSRWRPLVSTEGEKNDI
                     TR"
     gene            42706..43854
                     /gene="traJ"
                     /locus_tag="ACHKIR_000839"
     CDS             42706..43854
                     /gene="traJ"
                     /locus_tag="ACHKIR_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid transfer ATPase TraJ"
                     /protein_id="HFO9988623.1"
                     /translation="MISPDDFGTFPFSRFTAEEFRHFFAWCARHRVSDIDLTGGSPVS
                     VSRFGRRVRSSVNPLPSSIMMALSDELFGREVLPRVLSGHPVDRTIQVSGDASGRYGL
                     QRGQRVRLRCHLIQGTSATEEKAISVTMRVIPSDIPDILTMNIEPDLLDAMLRKTGLG
                     FICGETGSGKSTLAAALYRHILKNFPDRKIVTYEDPVEYILGGENDLLPPHQAEIGRD
                     VESFAAGLRSAVRRNPDIIGIGEIRDYETADAAVQAGNTGHFCIGTMHTKSPGETFAR
                     LLGLFPPEIRDSMAAATLSLVQFILVQVLVRTNDGGRQAVREYIVMTDELRNTLSGQS
                     HATWGHYIDEIIRQEKRRIRDQVLTMYQNDRIEKSEAALFIPAGEFPR"
     gene            43851..44141
                     /locus_tag="ACHKIR_000840"
     CDS             43851..44141
                     /locus_tag="ACHKIR_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFO9988624.1"
                     /translation="MKREGFWRDVTPPLNIYGVPLPFTMIYLLLFPYPSKTTFWVCSG
                     ILLFFMLLGYSGWTVRTLCIRLYSLLQGKQASGRPWWYRHRTELPREWHGPE"
     gene            44205..48221
                     /locus_tag="ACHKIR_000841"
     CDS             44205..48221
                     /locus_tag="ACHKIR_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD7 domain-containing protein"
                     /protein_id="HFO9988625.1"
                     /translation="MKSRYRTWLAVPPEETEAVKNAVPPLNGRKAVAWDPEKKLWYAE
                     AGTDLSLLKRWLPRPQEMSMDAGDPVTEFAQVLENAGLVIQGLPQMDGAIHRVATRDD
                     KKGAQSGAYKAYLDGRPSGWYRDYRSADNSPTTWTFSRAENIDPLARLHLKASAQQNR
                     DEKERKLQQQYNKQAGYARSYLNGLPQATAHEYLTRKGIRAAPGVRLNNKNELVIPFS
                     NGRGEIRSYQRIPVTGGKDARILKGSEKSGNWFAFGTPENGRPLLFAEGYATAASLHE
                     ATGLPVLMTIDADNMINVAKNARKIWTDSPFVFCADNDHQRKINKGVSSAKKAAASTH
                     GEVIIPTFTEAEKAQGLTDFNDLDASRGRDAFQRAMNAQLEYIGIPTPNNDTTDRREA
                     IVIGNLIFTPVKDEKPQVSPENRQSTAPEPAVSEQNHPYDIPAEETDNQAYEDDYAAY
                     ASLQSVTAGTASPNEPQVNAVSAIDSNAKNTSAATTDDRNVLAQETNADATVTTTAHD
                     TGTAETDSPSEPAEIQPVQQASPEPSAKDKPQENVVSATDNDAENTSSTTTALRSYDT
                     PETSVSDATGTQHTATTQPEEPQQVSMGSADDWIAFAEDLNADAPGNATVHSTDNAET
                     KTLSEPAGMHPVQQATPEPTEENKPQEEGVMSATDTGEESAAAAIDDDLNTVAEDLNA
                     DVTVMTTALHTDTAETASPSEPAEIQPVQQATPEPTAEDKPKEERPVSATETSTENEA
                     ARPETPSLFSRLRRFLTGGKSRTAEPPVIQENTLTEAETATEAAAPPSPPETSPEDTS
                     SAEPDTIIFAPRRPNSPQRHNLDEIIKNLTYDELPDNTALCRVDGEPAFLDKLNRLEM
                     VDGASADDKKVLAALAVATNFYGGVIELTGSDAFKQKAMQLIVEYDIKVRMKLPVQRA
                     ALEKLRQEKGIAHDAIVTHRPTPELNRLSPEETTMAQPAPPVSPKAETEQTAGSTAET
                     PHPEAPVTAKAEAPRAGTPEQPAASSSAPEKAPVSAQPQQPDTPEEEPPGKLRPGESV
                     TAVLTNYGEKEYAPGKGRCFFVELTNRSGKREYWGNGLEELVKNHQKGNPVTLTLQAR
                     ETFRLSGETRDRIRNIWAMTPVSNGITVDNDHPHAGQHILEFPMKTFSQVMSQMQQHW
                     PQIMQEVRSPKNPPNNLYIGEDRQPASAPQDQSMRLPLAGNAPNELIPAIASADKDNR
                     QLNLLLVHSADDHLQGVVRLNGTLYPALATPSADNRQLVINALTDNGLQFAGYGEAVN
                     HDENTHQRPSPQIMQFHLKQQDSPLFAAIHKPEEQPDKLFRSLGFEQTWKEWSDSQKA
                     EDRQEKTLQQAQSHSPGL"
     gene            48238..48588
                     /locus_tag="ACHKIR_000842"
     CDS             48238..48588
                     /locus_tag="ACHKIR_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFO9988626.1"
                     /translation="MNMKKTLLVILTSISLASPAAVAATCRAGAAAEAGSKAAWERAR
                     TASQAWSQRENDVASSLQSCLSRIKDVSITLPQFPSLDKILRDLENQVCDAVVDRVNE
                     HLPGNIDPWKDYNL"
     gene            48600..49295
                     /locus_tag="ACHKIR_000843"
     CDS             48600..49295
                     /locus_tag="ACHKIR_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotI/IcmL family type IV secretion protein"
                     /protein_id="HFO9988627.1"
                     /translation="MSEAHPTSPVSPETDNSGDIYKQTLSTIAQSEQRAALVPGLVRA
                     LLCSSAALVISLTGNIIQYWHNSNVTREYFATDNGRLVRLAPTSQPAWSQSDVMTFGS
                     MTLSEAFNLDFVHYRKQITTLAPRFSEAGFAGYVNALQASNILDTIKKEKFNLTATIG
                     AGTLVNQGQMENGIWFWKFQYPVRMRLVGQTSTRPEQAFTFEITVRRVDPRIKPGGME
                     ISQMVSRNAASGT"
     gene            49306..50280
                     /locus_tag="ACHKIR_000844"
     CDS             49306..50280
                     /locus_tag="ACHKIR_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotH/IcmK family type IV secretion protein"
                     /protein_id="HFO9988628.1"
                     /translation="MQIHHLLGALLIVCGTVTAADNPGWQNARTPQAGGKTGQAPGNA
                     PAGGPPPATQLTGPLPPPASPLVQEAASLDSPLNAGEIRELRGRMADNERAISAPVTS
                     VVPRISSLTVNLSPGASLPLVRTALHNVSVVTFTDINGSPWPQSDPPYNAAPGHFDVQ
                     FNGNMVSVTPLRAWAAGNVTVYLKGLSVPVILNITSGETDTNTLSQEIDSRLDLRIPR
                     QGPASPVVAVPSEKIALHDATLQAFLDGVPPDDPTVRRLKFSGNVPGTTIWQHGDDLL
                     VRSRAMLRDEFEQTLASADGTRLWKLPVTPLLTFSVNGQSIHVTPELE"
     gene            50284..51615
                     /gene="traO"
                     /locus_tag="ACHKIR_000845"
     CDS             50284..51615
                     /gene="traO"
                     /locus_tag="ACHKIR_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001272008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraO"
                     /protein_id="HFO9988629.1"
                     /translation="MSAEQDTAAKSGKKLSALLVLGGVIIIGGGYLLLTWLSPENSAS
                     LSSVNINSTTTGSGRTVVESQHYRDLLRADNERGAAEALRNNQTFIASLPRGLDTDHG
                     NNQRRDTQPADQAEGSGQPSTPPQADQTAREKQREALQKLLARINTQHPDARPPVVAA
                     AMWGTTGAPGISSHASPPGTGTSPQPRLQEASLATPAARTGIQLIPALTRVPAFIDTE
                     VDSDNAVSRVVASVPAGPWAGAVLYSQDARLAGKGMDIHFDRMMWRGMFLKVNAYALN
                     EHTGMSSVASDVNHRWFKHIVLPSVLGGVGSVGSLYKDANTQVIQGNYGSITGRVGMP
                     DGEALAGVIAGGMAERGSQLLTRQAESEPYRQVTVSRGEVVSILFVEPVMSNDISPPS
                     SGTAPSVPPPSGRPLTQEEAERQASDRIQAAVMRKQNEMRQRYGIQQESHP"
     gene            51612..52325
                     /gene="traP"
                     /locus_tag="ACHKIR_000846"
     CDS             51612..52325
                     /gene="traP"
                     /locus_tag="ACHKIR_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraP"
                     /protein_id="HFO9988630.1"
                     /translation="MKNEHEDPGSFGETTTPASGKSFLKRQYMGYSLPVWAGCLLIIL
                     AAVWYKFLRAPSEPPADAGFAVTPPAAESWAEPMPMTETGSGNTMSAPPVTPQADMQQ
                     LARDLKAELDRRDEEIRRTLTMLKESITTLGDTIRKDEAWAQETRQLLDETRKQVHAL
                     QMQGPLQIPTGNNGQSTKTPPSRLQGMQIMSVEKGMAWIRWQGSAWAVREGDRLGNIT
                     ITRIEPSSRSVITSAGTLR"
     gene            52322..52852
                     /gene="traQ"
                     /locus_tag="ACHKIR_000847"
     CDS             52322..52852
                     /gene="traQ"
                     /locus_tag="ACHKIR_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraQ"
                     /protein_id="HFO9988631.1"
                     /translation="MMRIDALTAIENFASGIFSAGMEFLFTWGELLGIIGLIGHLMRA
                     RAEGRHSMGPGKFIAGIVICGMLVALPSFINAGGTQMGFRADSFGPIAYVQPTTFGAA
                     AGAANAMLSLVKLAGVGFAMNGISIWRKSLLDGHTALSSSESISKGNVKFVAGVLLVF
                     IDRVIDASLASLGLAF"
     gene            52899..53297
                     /locus_tag="ACHKIR_000848"
     CDS             52899..53297
                     /locus_tag="ACHKIR_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001605438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6750 family protein"
                     /protein_id="HFO9988632.1"
                     /translation="MSRFRAMAARLCAAFIYVQLCLVACRDRLIRLLLLLPVILLPHT
                     AMANTLFDMIKTVGDGAKGSSSSFVAIAQVIGLVLFIAGLLGLKQIGKKPGAGLACIL
                     SIVVGACLAVVPEMMSSGQKTLGLSPVSIS"
     gene            53354..53605
                     /locus_tag="ACHKIR_000849"
     CDS             53354..53605
                     /locus_tag="ACHKIR_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003665769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraS"
                     /protein_id="HFO9988633.1"
                     /translation="MSCELPPFTLLRTEHGNVIARDNRDNQEFVFAECYSTAIAALIL
                     AAVTLYTERKTYEQNTATHRPGPGTLAFPAPATGHGIQR"
     gene            53520..54338
                     /locus_tag="ACHKIR_000850"
     CDS             53520..54338
                     /locus_tag="ACHKIR_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraT"
                     /protein_id="HFO9988634.1"
                     /translation="MNKTPPLIAPARERWLFPLPQPDTVFSGESLLPPPVLSTPGRCP
                     CCRRTVTHRFILEDSWPLQQMADTCRDTVVLLEKNLTRVMRLKKHPVPENADEKKKHT
                     RTLQDAERSLAQARLSARRLALRHVEKSQIVTTDALSENESELLQPEGPPFHLCAFCH
                     AWHCLNGYAAAQGVMVWLPDLHPASVVALNARALKEIFSDERKRIRQGRAVLNALVQN
                     RLAVEEKFRTWRPADFADALRRWPPAQRKTLREKMDGVALILMPDSFPDKKYVM"
     gene            54393..54752
                     /locus_tag="ACHKIR_000851"
     CDS             54393..54752
                     /locus_tag="ACHKIR_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9988635.1"
                     /translation="MDTLTAGYQFSADAFRRFIIRTLTDDIPPDGDEKEIARFILRHP
                     AFNGGWLLFLAAEIHERSRTHNSITVTVSVDNYLSWMDIAVSGLPELPDMPFCDFFIR
                     RQQFIYPGVPPTKSGEE"
     gene            54752..55132
                     /locus_tag="ACHKIR_000852"
     CDS             54752..55132
                     /locus_tag="ACHKIR_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9988636.1"
                     /translation="MKIHRISPETLITLILAHLAGKADSTAKEEHRLLRRFLRDDDGR
                     LAGILLNIAGILQFNRELSARHNYPATPLTEFSLRKRGKQLHLCLCSLRFFYIPPVFI
                     RNERRKCIVVHINKITYPPVNSLR"
     gene            55308..58352
                     /locus_tag="ACHKIR_000853"
     CDS             55308..58352
                     /locus_tag="ACHKIR_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO9988637.1"
                     /translation="MNINRIIFAIEDCISTLSRYSVSAFFPDYCDLHTVIGLDQQDRE
                     RRPWLKAPYIATTKQGYYLSVFEISGALKEMDENPDLTAPGTLESLITALAERMNTAY
                     KNIGHKISFVFERDPERGRNEIEVMLLPQRKSIARTGILLQDVLDEKVTTLTPWLVRE
                     RCWLTVWSSEELLSRTDRKDHQTRIRKLAEHAPPARFAQDPWRWTLSALKIRHDAFLD
                     TLEQALTHDSDGLLVRLMDIHEVGREIRRQLERNSTPAVWQPHLPEDARPAGWRQGED
                     TSVFHAPSLNLQLFTTQPETHGSLIKAGDLWHGMVAITLPPQNLRTFNQLVRDVPRAI
                     PWRIRMDLMPGGMKALGVKKTLLTYSSFISPLRPMYDSVMMLNDINKHDPVCIMTIVA
                     TTWGNSREVCTRNQALLKSALEGWGVCGTTTTFGDPRRAWVNTVLAASHGSGPVPLYP
                     PLSHALSLFPLNRAGSVWRGQGNLMMHTEDGSAWEVALASSQQNKHTELTPGAPGLGK
                     SVLINALSEIQIASAQKNLPFIAYIDKGFSAQGLVQLIRDSLPEQRKDEAVGIILSND
                     PEHTRNLFDVMYGAKKPATPEKNFMVSVLCALCVDTGTGQPCNPGDTRQIISNLVDLA
                     FREYGENNPRLYRAGTEPLVDLALEESGIAEQHDAGWWNAATWFEIRDMLHMAGNIPA
                     AQRAHYQAMPLLAEMSALLGQPSIRDVFGTVQRDNSAELLLDYIRRALDQGHSDYPMM
                     SGCTRFMLSPDTRVVAVDLNNVAGDKTPAGRLRTGIMYLLAGQIAGGDFVLPQYQEEI
                     RKNLDPRYHEVIFRRIEQLDQEVKTKVYDELHNAKGINFIWEALDTQELEQRKFGIRT
                     VLSTQLLQDYPPAVLKSANTLWLLRYRPDEIPFLRDNFGVPEVTLRRFLKMPEGAAPD
                     GSGVPVLAVFRVKNGTLARILKFTLGPLELWALNSSPKDSALRRALTQELGSLRARQI
                     LAENFPRGSATSLIEHRARTHDSENVIHDLAAELIRKQGYHL"
     gene            58352..58969
                     /locus_tag="ACHKIR_000854"
     CDS             58352..58969
                     /locus_tag="ACHKIR_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraV"
                     /protein_id="HFO9988638.1"
                     /translation="MYSIHITPRCRRPARGQVFLKTLRVDGDSFSFTLPSACFTLRPS
                     PNPRVIFASDRRADGEMSGVRLALPGETGTLITTATWQVNVPERPPFDIQHVIHWLLP
                     SPLTALMSDNTWLWQDSPGTPALTAGDWPVMETDTPTLKRVKRRTFTLIDNRNRQGWI
                     TRRFQCFPLAPLPESHSHRVPDILYPLKTEKKECKDNQQPHCLHS"
     gene            58930..60135
                     /gene="traW"
                     /locus_tag="ACHKIR_000855"
     CDS             58930..60135
                     /gene="traW"
                     /locus_tag="ACHKIR_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraW"
                     /protein_id="HFO9988639.1"
                     /translation="MQRQPATTLLALLIGCAVIPSTHAYSVTVVASQPVTTQIMPELA
                     AIQGVMAEMVRMQTATGTAINQSSEKLASVIAQDGQATRQQMIFSNETRRLEEARKSF
                     SVPDSICSESASGVAAESRRAAASAAARLSQGGGVSSKPIRERLSRAADSPIREAYDS
                     AGIHAGYCTEAEYARFGGTDVCPAVGELPGGDSQVRSLYQGAGTADTPAALTWDQKQI
                     DAATAYMKNTARPSAGRAPGKGEVGTQTGRTYVGLQNEYNGIIDAASHPQLSLIADST
                     PNEATRGALTEALQSPSAAAYFDRTASSEARTRGHMSQREFEAFEAGRRYANTDWQQD
                     LQGMEGDNLLRELLRTTALLNWQMNDLKEQIRQGNVIAGQQLALAARQYYGQRLGELS
                     QAMSQGSVR"
     gene            60132..60701
                     /gene="traX"
                     /locus_tag="ACHKIR_000856"
     CDS             60132..60701
                     /gene="traX"
                     /locus_tag="ACHKIR_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000650756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraX"
                     /protein_id="HFO9988640.1"
                     /translation="MKDDNSTDKKKPAPRLRRIGDIGINLFAPLAETRWMLRSCRHML
                     GQHKTRLKRLMPDRQPESPVCMTWEEAVRASGLTVNELDRRLRRRRMFWRTGFWLMMI
                     PVLLLCGMALAATALPAITLIRILACLLLMSAIAALCASKTLIVSYRLWQLNERRVSE
                     DEQGTFPDYLQARHGWKQATWLAITHRQY"
     gene            60774..62933
                     /locus_tag="ACHKIR_000857"
     CDS             60774..62933
                     /locus_tag="ACHKIR_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotA/TraY family protein"
                     /protein_id="HFO9988641.1"
                     /translation="MKIIPRALSAGLALAAVPAQAFVTYQDIVSAATNPDDLSRQALV
                     TIFGDVVTNPLTASSNTIIGNLFMVFNAIIAIMGIVWFLFIGIRHVVRTGHQGNVFNN
                     GRDVVGMLSIVSGFMMIVPTASGWSLAQLVMLWGASIMGIGSANIMVQTAAENIAEGY
                     SMTVQPVHVSTRTAARGILEMALCKHAMNAGLDDFNRTARSSTPPMSESSRTENGRYT
                     VTVSNGSATCGSVSLTVKGNGSNNQSALDYFFNPFIETEYQGVLTAQRSAMDTMLRDL
                     DSAAQEFVSAYRTRRDTGNGNLPDIESRIQKAANRYEETVQKALPTDNAETERREQLK
                     YYLTTYGWVALGAWYQTFATANQRLAELADRAPTVSGVSSQGEAGDTEFLKEVKGAYQ
                     AQLQNSPYTPALGTTASSTEYRTAEAAEPKSIIMKVWSGVNFADKIATSLSGTGTTSD
                     QLNPLIKMKNIGDYTMVTTETIWAGYTTVRVLSTAGSNSIFGRLVNAVSGLSDAAKAF
                     LEAAAPPLYFLLFLLFCAGFSLSIYLPFIPFIFWMTGIGNWIVSVLIGCAAGPLWAAT
                     HLGTNQDRGSRAAYGYIYLIDSMIRPLLMVFGFFFASVAIVAGGTILNALFGAALVNV
                     QADSLTGLFSIAGFLMIYARACTTLVAAVFALQAYLPDHVINFLGGRDGVNMLGNMVS
                     SVKDIIAGGNTNIRRTPGVREEKLKNINGDDKDGIKQ"
     gene            63015..63662
                     /gene="excA"
                     /locus_tag="ACHKIR_000858"
     CDS             63015..63662
                     /gene="excA"
                     /locus_tag="ACHKIR_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid IncI1-type surface exclusion protein
                     ExcA"
                     /protein_id="HFO9988642.1"
                     /translation="MNTTKSRYPSWWAYYQLARNTYFIFGIPCLLFYAFFGTLIVHDS
                     SLSQTRDFIFAYGCWALIILPLLWLYLKTRAKKKKVQQVVQQIKESSPFAPTNDYEQL
                     SFSKSCYFGIDIKNGTMLYVRIYPNNVMDVIGLDIHNFTRTVAEDGKLEIHTTYVSLP
                     MIPLEISGISARTLANTIHTMAARGYEYKERFPQMIRYRVKEWEKVAGVPVAEVF"
     gene            complement(63976..64128)
                     /locus_tag="ACHKIR_000859"
     CDS             complement(63976..64128)
                     /locus_tag="ACHKIR_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO9988643.1"
                     /translation="MPQRTFLTMLIVVCVTILCFVWMVRDSLCGFRVEQGNTVLVATL
                     AYEVKR"
     gene            64461..64703
                     /locus_tag="ACHKIR_000860"
     CDS             64461..64703
                     /locus_tag="ACHKIR_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001317518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9988644.1"
                     /translation="MSNEYQSTSINPDVFEQFLTLLNTHCKSSEAALAMLSTLCYELM
                     RRSGIHSLDSLMLNGEGILIQLQGADIREKTGQHIH"
     gene            complement(64796..66331)
                     /locus_tag="ACHKIR_000861"
     CDS             complement(64796..66331)
                     /locus_tag="ACHKIR_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO9988645.1"
                     /translation="MSCIKEKSKYAFERAKQFYFMLWCKESLIYLLFSVLVTWCCGYT
                     VKNALGNVLILFLMMLLGRRNVISATCVCIFLLLSAFYAPVGMIYGKINNSFIASVMQ
                     TNINEAGEFITGIPVLYFIVSLVIAAFTFWFWRKRDKSHRGFIFLLFFIVLSVNAWPK
                     RMVTNVMTGIAETREEMMRYESLKNNQKDSWDIVSVEKKYKTIIIVIGESVRRDYLSV
                     YGYPLPTTPWLNSAPGIFINGYFSAAPNTIGSLSRTLTLDYTETGNPGNNIVTLARKA
                     GYETWWISNQGSLGQHDTLISVIAANADKKHFLKEGSSSSLRIDDEDMLQYVREAVNS
                     SSPKVIFIHMLGSHTNPCDRLFNDAQPFRELFGHKVSCYLSTLLKLDRFLQAIYNMTT
                     EHGDDFAMLYFSDHGQSVSSDSTPVHHAPGLRSGYDVPLMMMASDITEHHLNHVHISA
                     RRFPEIFQWITGIQTKNLPLSFSLLQNDFAESVSVFNGERDVPVMSLPEEPILTGNAE
                     SEK"
     gene            complement(66531..66986)
                     /locus_tag="ACHKIR_000862"
     CDS             complement(66531..66986)
                     /locus_tag="ACHKIR_000862"
                     /inference="COORDINATES: protein motif:HMM:NF022214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 family protein"
                     /protein_id="HFO9988646.1"
                     /translation="MRDKSEVLTGAQKNRFGLILYIILLSVIYILITRLNLTTTYYSL
                     YSSLDENSQVTEYFIISIATFLFLAVLCLFFIYSFFKRKRVVGKLLLCIYILYISHYA
                     ITFVYCLYVVGAECMATGKIEHILIDIIIAMLSLSYVCLSGRARHIFVN"
     gene            complement(67074..67697)
                     /locus_tag="ACHKIR_000863"
     CDS             complement(67074..67697)
                     /locus_tag="ACHKIR_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001579788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO9988647.1"
                     /translation="MQERIDYHIEKYQFRARNESPRLMRQWADVMRECRESGAGARER
                     LRTALLNVDYVTSFELPFRLLLTRTPQMIDTLRREWLLSQKKALFNGRQRGCVYSLQN
                     DLSGVPDCFSYSLATRIRRMDCYGMTTRCFTDIAGQVKAPTARLKLALESGLLVTALD
                     GLFWLGTQRIAADVQRLRQSGMVIVTREVEVSDSLTGTTRLVSAYTL"
     gene            67846..68331
                     /locus_tag="ACHKIR_000864"
     CDS             67846..68331
                     /locus_tag="ACHKIR_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFO9988648.1"
                     /translation="MIKYIFALTLLLSGSVCADQIQGLVIRVLDGDTLEILQARHPVR
                     IRLVNIDAPEKKQPFGRWSMNQLKSLVVNTPVTVTYTQTDRYGRILGRVYTEDGMEVN
                     RWLVQHGAAWVYPDFNTDYTLPAYQREARTMKRGLWADKKPVPPWLWRKENKNSHNNY
                     K"
     gene            68975..69339
                     /locus_tag="ACHKIR_000865"
                     /pseudo
     CDS             68975..69339
                     /locus_tag="ACHKIR_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997724.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            69349..69540
                     /locus_tag="ACHKIR_000866"
     CDS             69349..69540
                     /locus_tag="ACHKIR_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000264907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9988649.1"
                     /translation="MWILIFWLSSPFNVSSGDARQPTLAVHSQEFRTEKACRDAFTAL
                     KKENVGEFALRGVCVPGGE"
     gene            69574..69897
                     /locus_tag="ACHKIR_000867"
     CDS             69574..69897
                     /locus_tag="ACHKIR_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgM"
                     /protein_id="HFO9988650.1"
                     /translation="MAYHSPDITLHYPYLRRMSWAQITAAAEDAEIHHDYARALILWQ
                     HAYHAATLTLNKNLAVAKIDFCIKRIRMREQMSGIIRKTGTDEILFRLSKHHHLYEKK
                     KTKEG"
     gene            complement(70040..70135)
                     /gene="dqlB"
                     /locus_tag="ACHKIR_000868"
     CDS             complement(70040..70135)
                     /gene="dqlB"
                     /locus_tag="ACHKIR_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinQ-like type I toxin DqlB"
                     /protein_id="HFO9988651.1"
                     /translation="MINLLIIVLRAVVAVANAVIAILELIRELID"
     gene            70634..>71847
                     /locus_tag="ACHKIR_000869"
     CDS             70634..>71847
                     /locus_tag="ACHKIR_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbA"
                     /protein_id="HFO9988652.1"
                     /translation="MSYQHSPTTEDPMNIWYIVGTICLLFAFLIWLFLPDIVFATCLI
                     LHTMWGMTDWGPFHHFAAPRYNLLANTANNAATITFSQWLDVMSQTVGILWLILLPMT
                     LGFLWMWFQHPAQPRFTRRPLNIHTLPHLFSSLSPAIAPVLADGDSNRLFHGQKRPER
                     RVALTPEVFVEQNNLIRNMQLDVASTRQCFMAQLGQPLTSWKDMAPHEKALFAIFGLQ
                     FFLGDRKAAVALMNNLNLSCRLKSKRDQGRFSTPVYSLARNAFIRVIKTEGAQQWLRQ
                     HRYVRSGLVWLYAHDLRLTPPNWLWLKGVDRTLFYALHRANTTKGFIEGAGVVAVARA
                     ENEASRLGLPCPEPCVEEAIEGLRRDMLGLGLIWDEPQPDRDRKRQIRTRWSLTDDVI
                     PRRHDNDEDTDTG"
BASE COUNT        18570 a        18757 c        17426 g        17094 t
ORIGIN      
        1 agcaaccaga cttaataagt caaaccaaac gcaattaagg agtcagtatg aaatttcagg
       61 ccattgtatt agcaagtttt cttgtcatgc cttatgcgtt ggcagatgac cagggcggat
      121 taaaacagga tgcagcgcca ccgccgcctc atgcaataga agatggctat cgcggaaccg
      181 atgatgcaaa aaaaatgacc gttgatttcg caaaaaacat gcacgatggc gcttcggttt
      241 cactacgcgg taatttgatt tctcacaaag gagaggaccg ttacgttttt cgcgataaga
      301 gtggtgaaat taatgttgtt attcctgcgg cagtctttga tggacgagaa gttcagccgg
      361 accagatgat caacattagc ggcagtctgg ataagaaaag cgcgccggcc gtcgttcggg
      421 tcactcattt gcagaaataa aaataaatta gcctgatggc ctaaactact gggccattag
      481 ggtattgggt tatgctattt gtcatccttt tatttacgat aatgcagagg taatttacag
      541 aattaaacac ttttttatcc gctgattttg ttttaaatca caatgttatc tcagagatga
      601 ctcataactt cggatttcta tgaaaatatt gacgtaattt tgcttttctc tcgttagaat
      661 agcgcgcaca aggaactgtg aaaaaggagt ggcaatgatc aagaagacaa cggaaattga
      721 tgccatcttg ttaaatctca ataaggctat cgatgcccac taccagtggc tggtaagtat
      781 gtttcacagc gtggtcgcga gagatgccag taagccagaa ataacggata accattctta
      841 tgaactgtgc cagtttggtc ggtggattga tcatctggga ccactcgata acgatgaatt
      901 accttacgtt cggctaatgg attctgccca tcaacatatg cataactgtg gtcgggaatt
      961 aatgctggct attgttgaaa atcactggca ggacgcgcat ttcgacgcct ttcaggaagg
     1021 gttgctttct tttactgcgg cattaaccga ttacaaaatt tatttgctga cgatccgtag
     1081 caatatggat gttttgacgg gattgccggg tcgtcgggtt cttgatgaat cctttgatca
     1141 tcagttacgc aacgctgagc ctctgaatct ttatttaatg ttgttggata ttgaccgatt
     1201 taaattggtt aatgatacct acgggcattt aatcggcgat gtagtattac gcaccctggc
     1261 aacttactta gccagttgga cgcgcgatta cgaaacggtt tatcgctacg gtggcgaaga
     1321 atttatcatt attgtcaaag cggctaatga tgaagaagca tgtcgtgcag gtgtcagaat
     1381 ttgccagtta gtcgataacc atgccatcac acattctgaa gggcatatca acattaccgt
     1441 gacagcaggt gtgagtcgcg catttcctga agagcctctg gatgtggtca ttggaagagc
     1501 ggaccgggca atgtatgagg gtaagcaaac cggaagaaat cgctgtatgt ttattgacga
     1561 acaaaacgtg attaaccgag tttaagctcc gtttaacatt cattgagaaa actgatgcta
     1621 ctgtgtcaac gatccgttat cagtgcagga aaatgcctgt tagcgtaaaa gcaaaacaca
     1681 aatctatcca tgcaagcatt caccgccggt ttactggcgg ttttttttcg ccgtcataaa
     1741 aatcaggccc cttgtacaca gttgtaacaa ggggccggtt aggtgaggga ttatctccgt
     1801 tcattagtca tcccatttgt ggctgaaata cgcggccaga aaaccagaaa ataaaattat
     1861 tcccagtacg gccataaaaa cattcatatt acccagcatt atttgcctcc gtttatggtg
     1921 tggtgtgttt cctcgcttac cttaattttc atctcaatga gaaagatatt agcagaggta
     1981 acctaaacga aggctgaatt aaatttttat tgtgttcgac ttaaatcaac aaagcgcggg
     2041 ctgcccccac ggtcttgcac gaattccttt taacatcagc acccacgcag caatgatcac
     2101 caacgctaag atgataaaca gcgagaaagg cggcagactg gtaagcacta tgccactcac
     2161 taatgggtta atcgccgcac caagccagcc taaagactga gcggaaaaat agctggcttt
     2221 cattcctggc ggcgcaatat ggtcaatcaa catatactcg cccggcgcat aaatgatttc
     2281 accgacagta aataccgcag ctgacatacc ccacaatagc aggctgttgc cggaaaaaat
     2341 aaaaccgacc agcccaatga cgaaacagag ggtgcctgct gtcatcagcg cgcggatgtt
     2401 agccgggtta agtcggcggc ccacggaata ttgcaacgta actaccatgg cagcattaac
     2461 cggaagaaca accgcaacca ccttttcggc aaaatcacca tcagcaatca ccatcacata
     2521 ttgtgaaatg cacgaggcaa atgcgccgct aacaaaggaa gccagaaaac cagagcaggt
     2581 aaaccacaac agtgctttat cttgtaataa aactttcggc gaccaggcac tgcctgtttc
     2641 cgtggcgatg attttctcgt tgcgctttac ccaaatttga atgaaaagca tgggaaacgc
     2701 ggaacagata gctgccagcc aaaagggcag attgatgctc tgcatcacca acaacgtgcc
     2761 gagcggcgga ccgatggtcc agccaatgtt gagcatggtg tagttgattg agaagatttt
     2821 cgttttgctg gtggacgaaa gattgtcggc aaaccaggct ttcaacacgg tagcaaaaac
     2881 ggaataggcg cagttaatga gggcaaaaaa gagcaccacc agcgtcacgt tattcactaa
     2941 aggaatggca ataaaaccgc tggcgaaggc ggtaattgcc agtaacatat agcgtttctt
     3001 gtcgaactta tccgccagga taccaaaacc gaggctaaaa acgacgccaa tagtgagcgc
     3061 aattgtcatc gcataaccga ttagatcgac actcaggttg tactggcgac tcaagtaaat
     3121 ggtcataaac ggcagcgtag cgccgcgtcc aatggttaat aacaacgacg aggcaagaag
     3181 ggcgctggta gagcgtcgta gggataagtt catttgtctg tccggcagtg cgtttcgtta
     3241 tgttttgttt tagagatgta aagaaatagc atggcaaaaa tccgaagtat agcgacttat
     3301 ttgcgcatta atgccgtcgt aaacggtgac taacgaatcg actgttaaag aaaagtaaat
     3361 tcagctaagt tgattgctta caaaagatga aattcagagg cggtataatg tcgcgaaaag
     3421 atggggtgtt ggcgctactg gtagtggtcg tatgggggct gaattttgtg gtcatcaaag
     3481 tggggcttca taacatgcca ccgctgatgc tggccggttt gcgctttatg ctggtcgctt
     3541 ttccggccat cttttttgtc gctcgaccga aagtaccact gaatttgctg ctggggtatg
     3601 gattaaccat cagttttgcg cagtttgcct ttctgttttg cgccattaac tttggtatgc
     3661 ctgctggact ggcttcgctg gtgttacagg cacaggcgtt ttttactatc gtgcttggcg
     3721 cgtttacttt cggggagcga ctgcatggca aacaattggc ggggatcgcc ttagcgattt
     3781 ttggcgtact ggtgttaatc gaagatagtc tgaacggtca gcatgtggcg atgctcggct
     3841 ttatgttgac cctggcggca gcatttagtt gggcgtgtgg caacatcttc aataaaaaga
     3901 tcatgtcgca ctcaacgcgc ccggcggtga tgtcgctggt aatctggagc gcattaatcc
     3961 caatcattcc cttctttgtt gcctcgctga ttctcgatgg ttccgcaacc atgattcaca
     4021 gtctggttac tatcgatatg accaccatct tgtctctgat gtatctggcg tttgtggcga
     4081 caattgttgg ttatgggatc tgggggacgt tactgggacg ctatgaaacc tggcgggttg
     4141 caccgttatc gttactggtg cccgtagtag gactggcaag tgcggcacta ttattggatg
     4201 aacgcttaac gggtctacaa tttttcggtg cggtgctcat tatgaccggg ctgtatatca
     4261 atgtatttgg cttgcggtgg cgtaaagcgg taaaagtggg aagttaataa gccccgatat
     4321 gtcggggcca gaacaaacta catagcgtgt tgattataat agggcacgcc gagcgcatcc
     4381 gacttatcac tgccagtacc catgtgattt aaatcaaacg gtggttgttc ctgcgatgtg
     4441 ggaacaacca cgacattgcc acaggaagta acaaccggct gcgtggtttg ttccgcaacg
     4501 ccctgcgcag aaaagagaat aagcgcagtt gctattgcgg ataaaagtgg tttcatgatt
     4561 gcctcagtga cgttgtcacg ttatcaacta gttgttgtaa tgatttaatg gatgtaaaaa
     4621 gcgcgattca ccctgcatat tggtcatccg gtatttatgt ggcggaacat caaagtaatt
     4681 tttgaaggtt cgggtcagag tttgttgcga ctcgaagcca tatcgttctg ccagatagag
     4741 tatcggctcg ttactttcct tcagcttttg cgcgatttcc gtcatcttac ggctgcggat
     4801 gtattggcct aatgaatgac cggtttcttt tttaaacatc cgttgcaggt gccatttgga
     4861 gtaacccgaa cgctctgaca ctttctccag tgacagtggc gattccaggt tgtcctcgat
     4921 ccagtccaaa atgctatgaa tggtaatagc gtcagtattg cgtctggaca tcgtcatacc
     4981 tctttttttg tttacggcag gactttctta agcaaatgct caagtgttgc cacttcgtcc
     5041 gccgtcaggt tttttgttaa ttcttgatgc aggtcctggc caactaattg atggcattgt
     5101 tcacatattg ccgcgccgct ggtggtaagt tttaccagta cgccgcgctt gtcattcggg
     5161 ttcggcaacc tttccaccca gcctttacag accaggcgat ccagcatacg ggtcagcgcc
     5221 cccaggtcga ccgacaatac ctttttcagt tcaaccggag taatacacgc cgcgcagcgg
     5281 atagagcaga gcaccttaaa ctgtgccgcg gtaatatcca gcggagacag atactcgtta
     5341 agcaggcgat ctttcttctg attaaccata tggattaagc gacccaatgg aataatttca
     5401 ttgaacagat cgctggtact tttcacatta gttgccctgg caagtaatta gttgcaaggg
     5461 ataatattgc ccaggcaagt ataagtcaaa tgaatgaatt gaccgatgcc acgttttgct
     5521 aaatcggttc aagttttggt caatgcgatg caaggttagc taaagagtga cactttatcg
     5581 ttgtttaccc cttatacttt tcgctgataa accagataca caggataaca accaccaatg
     5641 ttagatttat ttaaagcaat tggcctgggg ctggtggtgt tactaccgtt agctaaccca
     5701 ttaacaactg tcgcgttatt tcttggcctg gcaggcaaca tgaacagtgc cgaacgtaat
     5761 cgtcagtcgt tgatggcctc ggtgtacgtc tttgccatca tgatggtggc gtattacgcc
     5821 gggcaactgg tgatggatac atttggcatt tcgattcccg gtctgcgaat tgcaggcggc
     5881 ttaatcgtcg cgtttatcgg ttttcggatg ctctttccgc aacagaaagc gattgattca
     5941 ccagaagcga aaagcaagtc ggaagagctg gaagatgaac ccagcgccaa tattgctttt
     6001 gtaccactgg caatgccaag taccgccggt ccgggaacca tagcgatgat tattagctcg
     6061 gcgtccacag tgcgtcagag ttcaaccttt gctgattggg tactgatggt tgcgccgccg
     6121 ctgatctttt tcctggtggc ggttattttg tggggaagtc tacgcagctc tggcgcaatt
     6181 atgcggctgg tgggtaaggg gggcattgaa gcgatctccc gtctgatggg attcctgctg
     6241 gtatgtatgg gggtgcagtt tattattaac ggcatcctgg aaatcattaa aacgtaccac
     6301 taaaaaaggc ccaaatgggc ctttcaacta ttgcgtctgt tcttcgagtg tcactggcca
     6361 gcggcggaaa atgatgattg accatattaa cgcggcaaag gcaggcaccg cgcccacata
     6421 accaatcatc gacattgacc agtgcagact tacctgatta cctaccaacg cacccgcgcc
     6481 gattccaata ttaaatatcc cggagaatag cgccatcgcg acgtcggtag catctggtgc
     6541 cagcgccagc actttaacct gcataccaag cccgatgatc atcatcgcta tcccccagaa
     6601 aatactcagc accccgaggt gtatttcact gttcgccgca ggtagcagca gtgccaggca
     6661 caccagcaac agcgcaatcg ccgtacttac caacgcagac gcatactgat tacccagttt
     6721 accgaaaatc acgctgccaa taatgcccgc accaccgagt aataacagta atgccgtggc
     6781 aaagttggcg ctgaatcccg caatgttttg cacaaaaggc tcgatatagc tgtatgccgt
     6841 gtaatgggcg gtgacaacca ccacagttaa caaataaatg ctcatcaatg ctgggcggcg
     6901 gaataatagc gggaggcttt tcagcgaacc ggaatgctca ctgggcagta agggaagtaa
     6961 cttaatcagg cacaaaaggg tgataagctc cccgatacca atcgcgaaga aggtcattcg
     7021 ccaaccgaaa tactgcccca caatgcgccc gagaggtaaa cctaagacca tcgccagtgc
     7081 agtaccggtg gcaattaaac tcaatgcctg tgctcgcttc ccggccggag ccatacggat
     7141 cgccagagac gccgtaatcg accagaaaat cgcatgtgca aaagccacac caatgcgact
     7201 gatcaccaga acggtaaaac tccacgataa aaacgacagt acgtggctgg caataaacac
     7261 cacaaacagg cagatcagta atttgcgccg ttcaacctgg ctggtcatta acataaaagg
     7321 caatgacatt agcgctacta cccatgcgta aatggtcaac ataatgccga cctgagcggt
     7381 ttgcatgtga aaactctgcg caatgtcaga gagcaggcca actgggacaa attcggtggt
     7441 gttgaagatg aaggcggcga ctgccagcgt aacgacccgt agccacgcca ctttgcggga
     7501 aacagtgttt gttgtcataa cagattatcg ggtgagttgc gaaaaagtga tgaaatgatc
     7561 ttaaaacggt taatgatgtg aatacaatgc atttgtgatc tggatcgcat ttccatctct
     7621 ctgacggttg ttctgtcacg catcagagag ttgttaacat cacattttaa tgacgatcag
     7681 gaagggggcc tgttcttctt ttacctgagg tgcaatatta cgaacgggaa aatcatcagg
     7741 aagtgagatg aaattgctga tgcttactgg cgtaaccata tttgccccat atagtcataa
     7801 gtcatcggag catcactata tagagcatat tagccgcaag aactatcaaa aatagctcat
     7861 tactaactct gttttgccga gtcagacaca tccagtagtt gaataaatgc ctcaagcgga
     7921 cgcgttttcg caccgcgacg ccagaccagc caggttgtta accaacgcca ttgctcagct
     7981 aacggccacg cttcaacctg gtgatgcccc ggcatacttt ccagcattga gcgtggaata
     8041 agcgcaatgc ctgctcctgc gaccacacag gccaacatcc cgtgatacga ctccatctcg
     8101 tgaatagttc ccggagcggc accgtcagca tgaaaccagc tctcgaagtg gcgacgatac
     8161 gaacaattgg cgcggaaggc ataaatgtta ctgccattaa cctgactggc acgggttact
     8221 ggcgcatgtc cttgtggcgt gacaatcatc agctcctcgc ggtacaccgg tatcccgtca
     8281 atggcagtat ggttaatggg cccatcaata aacgccgcat tcagttttcc ctccagcacc
     8341 ccttccagca tagtgccgga agggccggtg gaaagtgaaa actgaatttt gggataacga
     8401 cggttaaatt ccgccagcat ggccggaatg cgcactgctg cggtgctttc cagcgaacca
     8461 agagaaaata aaccttgcgg ctcatcgcca gcgacaacgc tccgcgcttc atccactaac
     8521 gcgagaattt gttggctata gcgtaaaaag ttatgcccgg caggcgagag acgtaaacgc
     8581 tgattctcgc gaataaacag atcaaccccc agttctgttt ccagctggcg tagacgggta
     8641 gtgagattcg acggcacgcg atgcactttt gttgcagcct gcgtaatgct gccagcttcg
     8701 gcaaccgcgt tgaacatctc cagttgggtc agatccataa tattctcgat tcgtgaataa
     8761 gtggcttaat attattcatt ttaaagcaag agtaaatctg cgtatctttt taccataact
     8821 cataaaggag ataccccgat gaccattact ccggcaactc atgcaatttc gataaatcct
     8881 gccaccggtg aacaactttc tgtgctgccg tgggctggcg ctgatgatat cgaaaacgca
     8941 cttcagctgg cggcagcagg ctttcgcgac tggcgcgaga caaatataga ttatcgtgct
     9001 gaaaaactgc gtgatatcgg taaggctctg cgtgtccgta gcgaagaaat ggcgcaaatg
     9061 atcacccgtg aaatgggcaa accaatcaac caggcgcgcg ctgaagtggc gaaatcggcg
     9121 aatttgtgtg actggtatgc agaacatggt ccggcaatgc tgaaggcgga acctacgctg
     9181 gtggaaaatc agcaggcagt tattgagtat cgaccgttgg ggacaattct ggcgattatg
     9241 ccgtggaatt ttccgttatg gcaggtgatg cgtggcgcgg ttcccatcat tcttgcaggt
     9301 aacggctact tacttaaaca tgcgccgaat gtgatgggct gtgcacagct cattgcccag
     9361 gtgtttaaag atgcgggtat cccgcaaggc gtatatggct ggctgaatgc tgacaacaac
     9421 ggtgtcagtc agatgattaa agattcgcgc attgctgctg tcacagtgac cggaagtgtt
     9481 cgtgcgggag cggctattgg cgcacaggct ggagcggcac tgaaaaaatg cgtactggaa
     9541 ctgggcggtt cggatccatt tattgtgctt aacgatgccg atctggaact ggcggttaaa
     9601 gcggcggtag ccggacgtta tcagaatacc ggacaggttt gtgcggcggc aaaacgcttt
     9661 attatcgaag agggaattgc ttcggcattt accgaacgtt ttgtggcagc tgcggcagcc
     9721 ttgaaaatgg gcgatccccg tgacgaagag aacgctctcg gaccaatggc tcgttttgat
     9781 ttacgtgatg agctgcatca tcaggtggaa aaaaccctgg cgcagggtgc gcgtttgtta
     9841 ctgggcgggg aaaagatggc tggggcaggt aactactatc cgccaacggt tctggcgaac
     9901 gttaccccag aaatgaccgc gtttcgggaa gaaatgtttg gccctgttgc ggcaatcacc
     9961 attgcgaaag atgcagaaca tgcactggaa ctggctaatg atagcgagtt cggcctttca
    10021 gcgaccattt tcaccaccga cgaaacccgg gccagacaga tggcggcacg tctggaatgc
    10081 ggtggggtgt ttatcaatgg ttactgtgcc agcgacgcgc gagtcgcttt tggtggcgtg
    10141 aagaagagtg gctttgggcg tgagctttcc cattttggct tacacgaatt ctgtaatatc
    10201 cagacggtgt ggaaagaccg gatctgacgc tacatattca gctctgatat actcgcaggt
    10261 cttttcagac ctgcggtcca ggagtagaaa gtggcagtcg ccatggataa tgcaatttta
    10321 gaaaacatct tgcggcaagt gcggccgctc attggtcagg gtaaagtcgc ggattatatt
    10381 ccggcgctgg ctacggtaga cggttcccga atggggattg ctatctgtac cgttgacgga
    10441 cagctttttc aggccggaga cgcgcaagaa cgtttttcca ttcagtctat ttccaaagtg
    10501 ctgagtctcg ttgtcgccat gcgtcattac tccgaagagg aaatctggca acgcgtcggc
    10561 aaagatccgt ctggatcacc gttcaattct ttagtgcaac tggaaatgga gcagggtatt
    10621 ccgcgtaatc cgtttattaa tgccggtgcg ctggtggtct gcgatatgtt gcaagggcga
    10681 ttaagcgcac ctcggcaacg tatgctggaa gtcgtgcgcg gcttaagtgg tgtgtctgat
    10741 atttcctacg atacggtggt agcgcgttcc gaatttgaac attccgcgcg aaatgcggct
    10801 atcgcctggc tgatgaagtc gtttggcaat ttccatcatg acgtgacaac cgttctgcaa
    10861 aactactttc attactgcgc tctgaaaatg agctgtatag agctggcccg gacgtttgtc
    10921 tttctggcga atcaggggaa agctattcat attgatgaac ctgtggtgac gccaatgcag
    10981 gcgcggcaaa ttaacgcgct gatggcgacc agcggtatgt accagaacgc gggggagttt
    11041 gcctggcggg tgggactacc ggcaaaatct ggcgttggtg gcggtattgt ggcgattgtt
    11101 ccgcatgaaa tggccatcgc agtctggagt ccggaactgg atgatgccgg taactcgctt
    11161 gcgggcattg ccgtccttga acaattgacg aaacaattag ggcgttcagt ttattaatgc
    11221 agtctctcga tccactcttc gcgcgtttat cccgttcaaa atttcgctct cgctttcgtt
    11281 tgggtgtgaa agagcgtcag tattgtctgg agaaaggcgc gccagtcatt gaacaacatg
    11341 cggcggattt tgtcgctaaa cgtcttgctc cggcattacc ggctaacgac ggtaagcaaa
    11401 cccccatgcg cgggcatccg gtttttatcg ctcaacacgc caccgcgacc tgttgtcgcg
    11461 gttgtctggc taagtggcat aacattcctc agggggaagc gttaagtgag gagcaacaac
    11521 gttatattgt gacggtaatc taccactggc tggttataca gatgaatcag ccatgataat
    11581 tacggaaatc gataaaatag cattcccagg atatctttag ttgccgccgt tgtcagcgaa
    11641 catgctatcc tttctaagaa tttacttatt gcgttgagat aattgccacc cgcaattgta
    11701 tggacagata attaatgcat gtacagccca tttcaacttt ccgtttgttc caggaaggtc
    11761 atctgctacg taatagcatc gctatttttg tgctaaccac gctgttctat tttattggtg
    11821 cagagttacg gctggttcac gaactttctc ttttctggcc gctgaatggc gtaatggcgg
    11881 gggtgtttgc ccgctatgtc tggcttaatc gactgcatta ctatgcgatc agttatgtgg
    11941 cgatgctggt ttatgatgcc ataaccaccg aatgggggct ggtttcactg gctatcaatt
    12001 tctccaatat gatgtttatt gttaccgtcg ccttactggt cgcgcgggat aagcgtctgg
    12061 gaaaaaataa gtatgagcca gtaagtgcct tacggctatt taattactgt ctgattgccg
    12121 cattattatg cgctattgtc ggggcgattg gttcggtcag tattgatagt ctggatttct
    12181 ggcctttgct tgcggactgg ttcagtgagc aattctcaac gggcgtgttg atcgtgcctt
    12241 gtatgctgac gttggcaatt cctggagtgc tgccgcgctt taaagcagaa cagatgatgc
    12301 ccgctatcgc gcttattgtg tcggttattg cctcggtagt cattggcgga gcggggagtc
    12361 tggcgttccc gctgcctgca ttaatctggt gtgcagtgcg ctatacgccg caggtaacat
    12421 gtctgttgac ctttgtcacc ggtgcggtgg aaatcgtact ggtggcaaat tcggtgattg
    12481 atatctcggt cggttcgccg ttctccattc cgcaaatgtt ctccgcacgt ctcggtattg
    12541 ccacgatggc gatatgccca attatggttt cttttagcgt ggcagcgatc aattcgctaa
    12601 tgaagcaagt tgcgctgcga gccgactttg attttctgac tcaggtttac tcacggtccg
    12661 gtctttatga ggcgctgaaa agtccatcgc tgaaacagac acaacatctg actgtcatgc
    12721 tgcttgatat cgactatttc aaaagtatta acgataacta tggacatgaa tgtggcgata
    12781 aagtgttaag cgtgtttgcc cagcatattc agaagattgt cggtgataag gggctggtgg
    12841 cgcgaatggg cggcgaagaa tttgctgttg cagtgccatc ggtgaatcct gtcgatggtc
    12901 tgctaatggc ggaaaaaatc cgtaaaggcg ttgaactgca accgttcacc tggcaacaaa
    12961 aaacgcttta tctcacggta agtattggcg tcggtagtgg ttgcgcatcc taccgaacgc
    13021 tgaccgatga ctttaataaa ttgatggtcg aagccgatac atgtttgtat cgctcgaaga
    13081 aagatgggcg caaccgtacc agcactatgc gttacggtga ggaagttgtc tgacatcttt
    13141 attcattcac agcgatttta acggaatgtg ttctaccact ttattgagcg aataaaaggc
    13201 gtagttcctc gattaaacca tgatccgcgc cacacttttc tcataaaact gctcattaat
    13261 catcaggagt tgatcaaaac aggggttcgg cgtattaaag tttgctaacc tgtcgtacca
    13321 attaaaacag gttgtatgac taatcagaag ggaacccatt gtgaaaacac taaatcgtcg
    13381 cgattttccc ggtgcacagt atccagaacg tatcattcag tttggtgaag gtaacttcct
    13441 gcgcgccttt gttgactggc aaattgatct cctgaatgag cacaccgatc tgaattctgg
    13501 cgtggtcgtt gttcgtccga ttgaaacttc attcccaccg tcactgagca cacaggatgg
    13561 tctgtacacc accattatcc gtggcctgaa tgagaagggg gaagcggtca gcgacgcgcg
    13621 tctgattcgc tctgttaatc gtgaaatcag cgtctacagt gaatacgatg aattcctgaa
    13681 actggcgcac aacccggaaa tgcgctttgt tttctctaac accaccgaag cgggtatcag
    13741 ctaccatgcg ggcgataaat tcgatgacgc gccagcggta agttatccgg caaaactgac
    13801 ccgtctgctg ttcgaacgtt ttagccattt caacggtgcg ctggataaag gttggatcat
    13861 catcccgtgt gagttgatcg actacaatgg cgacgcgttg cgtgaactgg ttctgcgcta
    13921 tgcgcaagag tgggcactgc cagaagcatt tattcaatgg ctggatcagg ctaacagctt
    13981 ctgttctacg ctggtggacc gtatcgttac cggttatccg cgcgatgaag tggctaaact
    14041 ggaagaagaa ctgggttatc acgatggttt cctcgacacc gctgaacact tttacctgtt
    14101 tgtgattcag gggccgaaat ccttagcgac tgagctgcgc ctggacaaat atccactcaa
    14161 cgtgctgatt gttgacgata ttaaaccgta taaagagcgc aaagtggcaa tcctcaacgg
    14221 tgcgcacacc gggctggtgc cggtggcgtt ccaggcagga ctggataccg taggtgaagc
    14281 gatgaacgat gctgaaattt gcgcattcgt tgaaaaagcc atctacgaag agattattcc
    14341 ggtactggat ttgcctcgtg atgaactgga atctttcgcc agtgcagtca ccggacgttt
    14401 ccgtaatccg tacatcaagc atcagctgct gtctatcgcg cttaacggta tgaccaagtt
    14461 ccgcacccgt atcctgccgc agctgctggc agggcagaag gcaaacggca cacttccggc
    14521 gcgcctgact ttcgcattag cggcattgat tgcgttttat cgcggtgagc gtaatggaga
    14581 aacatatccg gtacaggacg atgcacactg gctggaacgt tatcagcaac tctggagcca
    14641 gcatcgtgac cgtgttatcg gtacccagga actggtagcg attgtgttgg cagaaaaaga
    14701 ccactgggag caggacctga cgcaagtgcc tggtctggtc gagcaggtgg ctaatgacct
    14761 ggatgcgatt ctcaaaaaag gaatgcgcga agctgtacgt ccgttgtgct aatactctga
    14821 acccggtttc gaccgggttt cttatttcac tatattagtt acaacatcct atgcgccttt
    14881 atcattaaca ggtgccacat gcagcacctg ttaatgttgt aagcttgtag gtaatttgta
    14941 agtagtgtct tgagaatgtg atcgtaatca cgtttaatag ggcgactttt ccacgcctga
    15001 agataaccat gattgttcgt ccacaacaac actggctgcg ccgtattttt gtctggcacg
    15061 gttcagtatt atccaagata tcctcgcgct tactactcaa ttttctcttt tctatcgctg
    15121 ttattttcat gctgccctgg tacacgcatt tgggcattaa attcaccctc gcaccgttca
    15181 gcattctcgg tgtcgccatc gccatttttc ttggttttcg taataatgcc ggttacgctc
    15241 gttacgttga agcgcgaaaa ctttgggggc agttgatgat tgcctcgcgg tcgttactgc
    15301 gcgaggtaaa aacgacattg ccggattcgg caagtgtaag ggagtttgcc cggctgcaaa
    15361 tcgccttcgc ccactgttta cgcatgacat tacgcaaaca gccacaggcg gaagtgctgg
    15421 ctcattatct caagactgaa gatcttcagc gtgtactggc ttctaactct ccagctaacc
    15481 gcatcttgtt aataatgggg gagtggttgg cggttcagcg acgcaatgga caactttcag
    15541 atatcctgtt tattagcctc aacgatcggc ttaatgatat ttcagtggtc ctggcaggat
    15601 gcgagcgcat tgcctatacg ccaattccct ttgcctacac cctgattttg catcgcaccg
    15661 tgtatctgtt ttgtatcatg ctgccgtttg cgctggtcgt ggacctgcat tacatgacgc
    15721 cttttatctc tgtgctgatt tcctacactt ttatttcgct ggattgtctg gcggaagaac
    15781 tggaagatcc cttcggtact gaaaacaatg acttaccgct ggatgccatc tgcaacgcta
    15841 ttgaaattga cctgttacag atgaacgatg aagcagaaat tccagcgaaa attcttcccg
    15901 atcgccatta ccagctgacg tgataattac tccgtacggc gggcaacaat aaacagacgc
    15961 ggaaatgcca gcaatatctg tccgttctcc tggaggggat attgctcttc cagcatctga
    16021 tggtagcgcg taagaaaatg ctgctgttcg ctctcggtca gatcctgtaa ccacggacgt
    16081 aatccagtgg cggtcaccca atcgataatc gactggtgcg acggcatctg gtgatagtaa
    16141 gtggttcgcc agatatcgac ctcacatccg gcttcactca gaatatcgta gtaagcatga
    16201 acgccagcca gcggctcacg cccgcgatcc ggataatttt gttcccaggc gacttcgcgc
    16261 atgagcacat gggttggctc tagccagtta tctggcatct gtactgccaa cacaccatgc
    16321 gaattaagta aagaaaccag atgagggaac aattcgtagt ggtcgggcag ccattgcagt
    16381 gaggcattag caaaaatcag atcgagtgcc tgttccggtt gccagttgcg gatgtctgct
    16441 tccacgaact ggcagtctgg caaagtactg cgcgcttcag caatcatcgc cggagacgag
    16501 tcgatgcctg ttatcctggc cgcaggccaa cgttgatgca gcagggtggt gctgttacct
    16561 gggccacagc caagatcggc aacgtatttg atattctcca gcggcactct ggcaagcagc
    16621 tccaccgccg gacgcgatcg ttcagcggca aagtgcagat ataaagaggg gttccagtca
    16681 gacatttatt tactcccgtc agcgcgttac cccaattgta gataaaactg ataaattcgc
    16741 ctcacggcat caacccattg aacagacgtt ttttacgcgg cccggtcatt aaagattcca
    16801 gtttcgcgac acaggttttg tagtggggcg tggttttatg gaatgccact gcgtcttcat
    16861 ctttataggc ttcgtagata taaaagcgcg aattcacttc cgggtcctgt aagacatcga
    16921 agcgcaaatt gccttcttcc tgtacagagc ccaggtggtt ctggcgaaaa acttcgataa
    16981 actcgtcaac cttatcttca tgaacgttaa tttcaaccag tgtgacgtgc ataattcttc
    17041 tccttaaatc cgcagttact gtttttcact cagatagagt tcatatgccc gatcagccgt
    17101 ttcgttatgg tgtaccaccg cctgtacggc tttcatcatc gccaccggat ggtcagactg
    17161 gaaaatatta cgccccatat ccacaccaga agcgccctga tcgatagcct gccagcacat
    17221 ttcaagcgcc tcgcgctccg gtaacttttt accgccagca ataacaatgg gtaccggaca
    17281 tccggcaaca atccgttcaa aacctttatc gacataataa gttttgataa tctgtgcccc
    17341 catttcagcg gcgattcgag tcgcgagcga gaaataacgc tgatcgcgca ccatatcttt
    17401 gcccacacca gtcacggcca tggtcggtat tcccgctttc attccggcat cgaccagctg
    17461 aataatattt ttgatcgact gatgttcata ttcgctgccg atataaacct gcgccgccac
    17521 cgcgcaactg ttcaggcgca cggcatcatc catcgataac gccacggctt cattacttaa
    17581 ttccgccaga atagagttcg cacctgacgc ccgcagtacc accggcttat tggtcgccgg
    17641 ggggactacg ctgcgcaaaa tgccgcgcgt acacattaat acatcggcat gttcaaacag
    17701 cggggcgata tttatatcaa tgcgttcaag tccggtagtc ggcccctgaa aataaccatg
    17761 atcaaaggcc agcatcacgg ttttacccgt tttcggatta aatatccgcg ataagcgtga
    17821 ctgcattccc caatccagtg caccgcaacc tttcagggta aaagggatat tttgttgcgg
    17881 ttgatcggta cgaaaatctt taccatcttt aatatcatct aaatctgcca taacgtactc
    17941 cggttaagta atgcacatca gaaatcgtat ttgccgatat tctctttgtt gaatatcacg
    18001 cgctccggta acagtacgat gccattacca tcagcttcgt agtcatagcc ctgaacgctg
    18061 tttggcgaga cttccacctg acctacgccc tggatatcca gcttgtcgcc cgttttcatt
    18121 gatccttttt tcaataatgc atccgcgaca taaactgata ttttgccttg ctgaaccaca
    18181 tcccacaggc caaattcttt caccgtgccg cgttccacat atggacgcat cacgtttggc
    18241 gtactgaatc cgacaatcgc aactttgtca tttttcaggt tttctgcggc ttgtgcggca
    18301 gcgggcaggg cattggcatc gggggcgata atggcgtcga gatcgctata ggcttttaat
    18361 attccttctg cggtttgtaa cgatttagtg gcatcgttat agccaaactg cgtagtgaca
    18421 atttcccagc ctggatgctc tttggcgatt ttcgctttcg cttctttcac ccactggttt
    18481 tggtccgtaa cggtggggct tgagtagaaa aacgcgactt tggctttgtc tttattcacc
    18541 tgacgcgccg ccatatccac caacatacct cccaactggg cgggcgttcc ctgattaatg
    18601 tagtaagagc ggcactccgg tttagtatca gagtcccagg tcagcactct cacaccacgt
    18661 tgcatggcgc gtttcagtgc cggacacaag ccatcaggcg aaaccgcaga aacgataatg
    18721 gcgttatacc cctgattgac gaagttatta atcaactgta cctgaccaga aacactgggt
    18781 tctgtcggcc cgtcgtaggt cacatcaacg cccaactctt tacccgcttg ttgtgcgccg
    18841 ttgccaccgc tggtaaaaaa tcccacgcca accagtttgg gaataaatgc aatacgctct
    18901 gccgcctgca cattcataga gattacggca atgccaagcg cgctaagtaa ggcgattttc
    18961 ttaaagcgat gaagtgtcat gaagatatcc tttatggcaa tgggttattg gcccgacgcg
    19021 ccagccactc tttaatttgc tggcgatgca ggctaacgga acgacctacg acaacgacga
    19081 taagtagcgc accggaaagg gcgctggaca cctgatttgg cactcctgcc atttgcaaac
    19141 cttgttgcaa atatcccact aataaaaccg ctatggcggt gccgataatg gaaccagaac
    19201 caccataaat attggcaccg ccgagcacta cggcggtgat ggcgggcatc agaaacgacg
    19261 caccgagatc ggaacgtgct gaaccaaaat acgataccag cagcacagcg gcgaccgcag
    19321 acgccagccc cgtcatggca tagagcgcac acaaggtgcg gttaactgga atcgcgctat
    19381 aaaccgccac acgcgggctt tgcccaatta aaaacacgtt acgtccggca tgggttttat
    19441 gcagccagag ccagaaaacg aggagacata tcaggaagat aatcagcgga acggggagcc
    19501 ccagcacatc caggttagcg aaatctgtaa acgccatcgg gaatccacca ataccttcgt
    19561 accccgtcgc tccggccatc ccggaaagca gcagggcgct tccggcaaac agatacagcg
    19621 tgccaagcgt aatcaccagc gggttaactt tggtatagat aattaatccg gcgttgatca
    19681 gcccgcacaa tgcgccgagc agtaaggtca gaagaatcgc cagcggcatc ggcacaccac
    19741 tttgaaacag tacgcccaat gcaatggcgc agaggccgat ggtcgaacca aacgaaatat
    19801 cgatcccgcc actgacaatc accatcgtca gcggtagggc gacaatgcca atgcagataa
    19861 agtcactggt gctgaacagc aacatattga gatctaacat ccgcgggtta attgcaccaa
    19921 atgcaacaat ctcaataacg agcagtgcgg caagagccag ttcccaaccg tagcgaatac
    19981 gcattacgcg gcctcccgtt ttttacctga cgaagcgggt ttaacggatg gcggtggcat
    20041 cataaagcgg gcatattttt gccgccgcag attacgttcc agcgcacaac gcaggcgtcc
    20101 atcaaacacc agcaccgcca gcagaaccat gcccgcgata aaatcattcc accatgccgg
    20161 aatgcgcaac agcaccagta cactatcgat ctgcgtcagg aaccatgcgc cgagtaccgc
    20221 accgatgatc gcgccggaac cgccgagcaa actaatgccg cccagcacgc aggctgcaat
    20281 tgctttcatc tccagcacgg taccggtctg gttggggata aaaccaatct gcgaagcaaa
    20341 cacaattccc gccagtgccg ccatgcagcc gttcaacgaa aatgccacaa tgcgaatggc
    20401 ttccgtacga acgcccagtt gacgagcgcc ctgtaaatta tcgcccgtga cataaaaact
    20461 gcgtccaaac gccgtctttg ccagcagcca ggccataaat gccaccagaa ttatcgtcaa
    20521 ccaaccaatc gcggaaacgc caaacagcag cggggcggag agttgtttca gttcggcggg
    20581 taatccctca atccatttac cgccagtcca cagcaacatg atgcctctgt acaaccctaa
    20641 cgtgccaagg gtggcaacaa tggcagggat ctttagccac gcgaccagga caccgttgaa
    20701 aaatcccgcg agcaaaccaa gcagtaaagt cgcgacacaa gcaacaggta gtgaataccc
    20761 cgcgttcagt aacatcccca acagcaccgc gcacattccg gtaatcgaac ccactgaaac
    20821 atcaatattg cgcgtaagca ttaccagcgt cgcgcccatt gccagcagga tcaggatttg
    20881 cgcgctgcta taaaccatgg tcagcgtttg cacacttaaa tactgacggt cgagaaaacc
    20941 gggtaataca aacagcagca ccaccgccag cagtgccgtg atttcacggt tgttctgaat
    21001 aaatttcagc atgacgcctc ctgacgctga ctatctccga aggcaacgcg cataatggtc
    21061 tcgacattaa tatcgcgtcc ggtcagtgca gagtgggcaa tttcgccctg atgcatcaca
    21121 tacacgcgat ctgccatcag ttcgatctct tccagatcag aggagataag cagcacagcc
    21181 acattttgtg cggcgatgct gcgcaacagt tggtagatat cattacgcgc cgagacatcc
    21241 acgccgcgcg tcggctcatc gacaatcaaa acttgcggcg aagcttccag acatttggca
    21301 atcaggattt tttgctggtt gccgccggat aaggtccgcg cagcttgttc cggttggtta
    21361 aatttaatat tcagcgcccg gcgatatcgt tccagggtgg cattatcttt cgcggttttc
    21421 gcccagaatc cacgaaggtt atgagtaagg gcgcagacgt tccacgccag tgaagcatcg
    21481 agattaagtc cggatgactg gcgatcttcc ggcagataaa ccagaccgcg cagtaaacga
    21541 tctccggtgg ataatctatt gatctcttta ccattcagca taatgcgtcc gccacgcaaa
    21601 gtacgcagac catagagcgt ctcggccagt tctgtgcgtc cggctcccac cagcccagcc
    21661 aagcccagaa tttctccggc attgagcgtc aggctgacat tcctgaaacc ttcgccggtc
    21721 agattttcca gtgtcagcac cggcgttccc gcggcatgtt gtgggcggtt gccaggtaat
    21781 tccagccata atttttggct ggcagagagc gatttttccc gtagcgctgg cgtgatggcc
    21841 tgaataatgt cgtcggtaga cagttcgctg gttttgccgc ttaaggcgat ggttccgtcg
    21901 cgcatcacgc taattcgatc ggcaatctgg cgaatttccg gcagcttatg cgagataaaa
    21961 acaataccca cgccagtagc aagcagctct tgcaagcgac gaaacaaacg ttcggtttcc
    22021 gcaggggtaa gcgaggctgt aggttcatcg aggatcagaa tccgcgagtc gcgcatcagg
    22081 ccgcggagga tttccaccat ttggcgatcg gcgacatcca gcgatcctgc cagactatgc
    22141 agatcaaact ggcagcccag cgccaccagc aagttattca ttttctgtat ggagagttgt
    22201 ttttttgcca gaccaaacag gatgttttct tttatcgaca ggcttggaaa aagcagcggt
    22261 tcctggggaa cgagataaat gcccagctga tgagcatgaa ctggcgttaa tctggtgtaa
    22321 ttattgcccc caatctccag cgtaccgcta tcagcagggg taataccggc aataatcttc
    22381 attaacgtcg atttaccggc accattgccg ccgagcaggg cgtggacctc cccctgatgc
    22441 aacgtaaaat cgatgccttt caggacatta acccctgaat actgtttata aaccgagcgg
    22501 gcgcaaagta gcggtaacgc gcgggtatca ctcgtttgca tatttccccc gttcagtttt
    22561 gcaggtgagt tttgaacaaa tgtatttctg cttttaattt gttcataacc ttaggtggac
    22621 attgcacata tttccgacga atagatcaca atttatgcta ttttgatttt cacggttgcg
    22681 tttgttcatg ctcgtagagt caaactgtgg ttgctatcac agatataaat gcgcaagaac
    22741 tgaacaattg cattaaagat ttaaatatgt tcaaagtgaa gaatgaatta tgacaatcaa
    22801 cgattcggta atttcagaac agggaatgtg tgaagaagaa caggtcgcgc ggattgcgtg
    22861 gttttactac cacgacgggc tgacccagag tgagatcagc gatcgtctcg gcctgacccg
    22921 tttgaaagtg tcgcgattac tggagaaagg gcatcaatcc ggcattattc gcgtacagat
    22981 taattctcgc tttgaaggct gtctggaata tgaaacacaa ttacgtcgtc agttttcgct
    23041 gcaacatgtc cgggtgatcc cagggcttgc ggatgctgat gtcggcgggc gactagggat
    23101 aggcgcggcg catatgttga tgagtttact tcaaccacaa cagatgctgg cgattggttt
    23161 tggcgaggca actatgaata cgctgcaacg cttaagtggt tttatttcgt cacagcaaat
    23221 tcgcctggtc acgctctccg gtggagtcgg ttcttatatg acgggtatcg ggcagcttaa
    23281 cgcggcgtgc agtgtgaata ttattccggc tccgttgcgg gcatcctccg ctgacattgc
    23341 ccgtacgcta aaaaatgaaa attgcgtcaa agatgttctg ctagccgcgc aagcagcgga
    23401 tgtggcgatt gtcggcattg gggctgtgag tcaacaagac gacgcaacaa tcattcgctc
    23461 cggctatatc agccagggcg aacagttaat gattggtcga aaaggggctg ttggcgacat
    23521 tttaggctac ttttttgatg caaaaggtga cgttgtcacg gatattaaaa tacataacga
    23581 actgattggc ttacctttaa gcgcgctgaa gaccataccc gtccgggttg gcgtggcagg
    23641 gggagaaaat aaagccgaag ccattgccgc tgcaatgaaa ggcggctata tcaacgcact
    23701 ggttaccgat caggacacag cagcggcgat tttacgtagt taaatttgca tgaccttgtt
    23761 accgggaatg atggagaacg tatagaggaa cagacagaac gcataagccg aggataatct
    23821 aatggctcga ctctttaccc cttcagaatc aaagtactac ctgatggcgc tggatgcagg
    23881 caccggaagt attcgggctg tgatattcga cccggaaggc aatcaaatag ccgtgggaca
    23941 ggcggagtgg cggcatctgg cagtaccgga cgttcctggt tctatggaat ttgatctcaa
    24001 caaaaactgg caactggcgt gtgagtgtat gcgccaggcg ctgcacaacg ccggcatagc
    24061 cccggagtat atcgctgctg tttcggcatg ttcgatgcgt gaaggcattg ttttatataa
    24121 caatgaagga acaccgatct gggcctgcgc caatgtggat gccagagcgg cgcgcgaagt
    24181 taacgaactt aaagaactgc acaacaatac ctttgaaaac aaagtttatc gagcgaccgg
    24241 gcaaacgctg gctttaagcg ccatccccag attactttgg ctggcgcacc atcgttccga
    24301 tatttaccgt caggcatcaa ccatcaccat gatcagcgac tggctggcct atatgctcag
    24361 cggcgaactt gcggtggatc cctctaacgc tggcaccacg ggacttattg atctaaccac
    24421 ccgtgactgg aaacctgcat tgctggatat ggctggcctg cgtgccgata ttctttctcc
    24481 tgtcaaagaa accggcacat tgctgggtgt ggtaagttca caagcggcgg aactctgcgg
    24541 tctgaaggcg ggcactccgg tggtcgttgg aggaggtgac gtgcagcttg gttgcctggg
    24601 gttaggcgtt gtacgtccgg cacaaaccgc ggttcttggc ggcacattct ggcagcaagt
    24661 tgtaaactta gccgcgccgg tgacagaccc agaaatgaac gtgcgcgtta atccccatgt
    24721 tattcctggc atggtacaag ctgaatctat aagctttttt accggactca ccatgcgctg
    24781 gttccgcgat gctttctgtg ccgaagaaaa actgattgcc gaacgtttag gcatcgacac
    24841 ctatacgggg ttctgcgccc atttcctcca aaagtctcac tcttagcata aaaaccatta
    24901 tttatcatgt tgttaaccgt catattcgga ctcaatatct ccgatatcac ttagtgggtc
    24961 tactccctcc aaaagactgt ccaaatttgt gatcgcaact tgatcttcaa aagaataatt
    25021 ccctccaagg agaatgtgtc tccatgctac tggggataag ttagccagaa catctatcgc
    25081 ttttttatta ttttcttttt caaatttctc gaccagcctg gataatattg ctgaattata
    25141 atagattaca caattagcta ttaacctcgc acagtcgttc cattgatcta tttggtagtc
    25201 attaccaccg cgaaattgat ttccatttac tgatgctatt gctcttctta gttgatgata
    25261 agcttcacct ctgttcagtg cttgttgcac aaattgcctt aaggttttat tgtcagcata
    25321 atccaggaca tatatgcatt ttataactcg gtcatactcc cgaagtgctg ctaatgtctt
    25381 gttgctacgc tttgtattag ataacttctt aacaaccgta ctttgaatgg ttgtttttct
    25441 gcttaaagaa caaataatat gttggatgtt ttcccattca tgttcaatga gcttaaagtt
    25501 aaattgtttt ttaagtttta taatgagttt atcactctcc agtataacat caaataattc
    25561 attaaatatt ttcttgaatt ttgcataacg tggtgaaaac tgatagccaa aaatgtcaag
    25621 aatcgcaaaa ttaacgttgt ttataccatg attgtcagtc gctaatgact ttggttgtat
    25681 ttcactggtg ttgttatata acaagtcaaa tgcaaaatga ccttcatatt catttggaga
    25741 aattaccaca gttgataatg gcacatgatt agataccagt gtcatcgcag aaaccccttt
    25801 cccctttctg aaatacttgg cagaataacg ggctttaaat gtatttattc tgcaagtgtg
    25861 cttctgccca tcaacacttc caaatggaga atcttcattt atcgtccagt atttatatac
    25921 agaaagattt gcaattgcat cagatattac tgtgttggcc attcctgttg tttctggacg
    25981 aatgaatttg tcattcacat cacgtagtat tccaatacta cggtctgatg cagatgccat
    26041 tctgtgaaca ccataatttg cgccattacc aaatatacag gcaagaaggt cattatagtc
    26101 agcttcaaga ttatttttcc tggatgatat ggcattaaac acagacaaaa aattcgtctc
    26161 attgtttaca tacttcatta cgtcaactat tccgatattt tttatctgat tgtaaatcgg
    26221 attatcgatg ctggtcttcc attttttact ggccagactc cactgtaatc tgttggagtg
    26281 tggttgtttt ttcacaaact cattagcatc tgagtttatt ccaatggtta tacgagccag
    26341 tttatcgcta aaggtattct ccagctcatt aagagtctgc gttattggtg tgataagtcg
    26401 ttcaaggcct gttttctcta tcaaaatcga tttattctta tgccagtaac ttcgttctat
    26461 gagatcatct tccagtctct tgttttgttc actctccgca gcataaaaga caccgttttc
    26521 cagatggtta gataccctgc tgtacatatg aaattcgaat cgttgagtgt taatttctcc
    26581 ttcttttaca atgtaatttt tctcggatgg atgaattacc ctcagatcct tagcatccag
    26641 ctttcctttt cctctgagat catccttggc agcagtaatt tgctggccaa taacagactt
    26701 ctcgttgtca tatactatat ctatagccag aaaaagcttt cggattgaat tggcgatctt
    26761 aaggtgatgg ctgtcaatat actgccattg atattcaatt acatcgattt tatttttgtc
    26821 aaaaaattca ctcacaagat gcatatcatc tttcgatata agtgaaaaaa ccttttcacg
    26881 aacatatcca aaagggatgc tgtcttcaat gttactatca acgaagaagt taaatatttt
    26941 actcgcaaac ttaagctttt tatttattag atctatttcc tctgttgccc gagcttttgc
    27001 aaacaaaact gaagcgtctt tatgtttttt tactaaatat ctgaaggctg tgattatttt
    27061 atcatttatc tctctgtatc tgaagtacaa gtagcagcac aaataaagta ttccttgcca
    27121 tttagggtgt cgcttaagtc tgaatgttgt actgtgtttt acgatagttg catagtactc
    27181 catgtttccc tgggatatac ctaatgaatt aattatatct tttatttcat agtaaatact
    27241 acttatcaca ttgtgagttc ttaattcatt ttctatttct gatgctgaga agtctctcgt
    27301 gctataatta tatcccctga tggagtttaa tattccatca tcatggatga tggatgtaat
    27361 cttttgtgca gtggcaggat aaattctctt tgacaaaata ttaattgtcc ttttcctttc
    27421 aaagtttaaa gcatctgaaa cgaggttctg gacagtactg tatccaggaa gagctatacg
    27481 ccgctgtcct aaaaaagcaa gtaattcatc aaaaatatat tttggttcca tacatatagt
    27541 agcaacatca ttcagacgtt cacttaactc attttttata gatggggtga gttgttcaaa
    27601 tcctagagta gaaagcattc gtttaacaag gcggtacttt gtacttttag gtatgtttaa
    27661 attgagatta gtactttcgt ttttataata tgtctggata atatatttca catccctttt
    27721 tacgtctttt aatgtaaatt caggaatcac aggtttatat ctgaaatacc ctgttattaa
    27781 tatcagatac accctgcttt ctatatttat tattttatca atttctcttt taaggctgat
    27841 atccagcgaa aaaaactcat ccctttcaac aggattaaaa gaggggactt gatataattc
    27901 atttatttca ctttttgtta atatactaat tcttttcttg tacttctgca tacatttacc
    27961 aaaatgctaa atatttgtta aatatggcta gtatcaaata ttaagatatc tgatacctgt
    28021 ctaaaacagc ataaaagcag gtttcaaaaa tcgtattttg tacattatga taccatgcag
    28081 tgtcaaaaac gataagtgag actaaaaatg gcgttattgg ggtatgcacg ggtatcaacc
    28141 agtcatcaga agctaacaca tcaaatctcg gagctaaaaa atgccggggt acgggaagat
    28201 cgtattttta cggatatgat gactggcgca actgatgaac gggaaggact acagcggttg
    28261 ttggcccgtg cagagaaaga cgacgtaatc atctgtacaa aaatggaccg actggggcgt
    28321 aacactgctg acatgattcg tgtggttgat agctgttaca aaaaggggat cgccatccgg
    28381 ttcctggaga acggtctcag tacagaaggg acaatgggca aaatggttat ccaaatcctt
    28441 gctgctgtag ctgaagcgga acgtgagcgt attctggagc ggacaaacga cggaagaata
    28501 gcggcacttg ccgcaggcgt aaaatttggc aggaagaagc atccacgaac gcctacagcg
    28561 ttggagctta tatcacaggg agaatctctg ggcagtgtga cagaaaaaac aggtatctct
    28621 cgatcgacgt acttcaggtt aaaaagaaca ataaaaaatg atgctaaaat tgcaacgttt
    28681 tataaataaa ttcaattaat tacctaagca aggtgagact tttagaggaa atgggcgcga
    28741 accccctatc tcagtaatga agatgccgat acccacacca tggtagccac tgtcagtcgc
    28801 cgcatactgc ccggaaaacc ccgtcagtgc ttctccctcg ccctgctgat cctgccacta
    28861 cttgaacatg gcggatatgc catcacggaa ctgacgttac ccggagaaac accgctctat
    28921 agctttgttt ccgttgtcga cggtgttctc gtcagtgacc tggtgggaag cggggaagag
    28981 gttcgggaag cccgggatac ctttctttcc atcaacaccg aaccgactca gggctggata
    29041 cactatgaac cagcggcatt ttcagcaggc gctcagaatc agcacctgcc gctggcctcc
    29101 ctgactggct ctggtaaaca tccggctgcc gcacgcctga atccggtatc ccggggaccg
    29161 cagttcatca ctgtctctct gatcatcgcc cttctcggca ctgcatggta cggctggcag
    29221 tattatgaac gctggcagac agaaaaagcc gcgcaggcag cggttgcccg acaggccaca
    29281 gaggaacgaa tcactccccc ctggcccgga ctccctgaaa cagacgcgtt catccgggga
    29341 tgtgctgata tatggacttc actgccactg tctgctgccg gatggcgtta ttcgctggca
    29401 gaatgctcga ctgacggtgg cagcggcaat cttcgcgcat cgtacaccaa tactgcgggc
    29461 gcgacagtga ctgacttcat ccggcgcatc agtgatatga cagacagccg accgtttatt
    29521 gtgatgccgg aaggaaagac cggcggcatt ggcctgccgg tcaccttccg gctgtctgaa
    29581 aacgccgtca cagttgatgc actaccggaa accgctgtcc tgcaggaacg actgacctcg
    29641 ctggctcagt caatgcaact gaaactggac tggcaggaag ttaataacag cctcactgat
    29701 gagaacggac agatcattca gcccccatgg aaagagtatg accttcagat gcagaccaca
    29761 ctccctgcac accatctggc tgaacgcctg aaagagcctt ctgtccgttt tatctctgtc
    29821 acacggcaac tggagaacgg tcgtttccac tatcaattta caggtaaata ttatgcccga
    29881 taaagccaaa acccttctgc tttcagccct gctgttaccg gcaacggcaa tggccacaac
    29941 cggaaatgca gagcaacaga acacacagca aataaggatt gaacagatac aggaagagac
    30001 agccctgtat gaagccatgc tggcgagaga gaagatccgc catgaactgc gaacacttga
    30061 gcagggcgtt cccgcagagg gcggacgtca gcaggaaagc atttccgcag ttacaacaaa
    30121 tgcacaggca ttaccatccc cccgcctgac agcaatattc ggtgttcagc ctcaactgac
    30181 tgccaggata gaacttccgg ggggaagtat ctccgatgtg aaggcgggcc agaacatccc
    30241 cggaaccact tatcacgtgg aaaaaatcac gccggacagt gtagtgctgg aacagtctgg
    30301 caccagaaaa accctgcgac catgagactg gccgccgtaa ctggcggctg tcaaaaataa
    30361 aaggatgaaa gacatatttt ccgggcaccg ctgcccgtaa ggaggcttta tgctggatcc
    30421 agcagatctg ccgccggatg atattctgga ttatgtggcc atcgacaccg acaacaccgg
    30481 acaactgcgg atcagagttg tggaggggaa aaaacatctg cgtgccgtcc aggaatatct
    30541 gacccgcctt cgcgcacgct atcaggggcg tgtgggagac tttgagttca ccacgctgga
    30601 tgtgatagcc cgcctcagac aggacgccac aacgacaggc gatgaaaacg ctattaaccc
    30661 gatacagcag aaaatgctcg gctatatccg ccattctgcc tcccttggtg cctccgacct
    30721 tcatatgacg ccagggcggg acaacacaga cttcacctat gtggaagccc gggttcatgg
    30781 cgaactggaa gtgctcgata tcctccggaa agaagaaggt ctggaattac tgggggctac
    30841 ctattccggc atgacggatg tcatcaaggg cacacaattt gatcccgggg ttccacagga
    30901 cgcacgtctc tctgaacagt acctgaaact ggctggcctg tttggcgcac gctactccca
    30961 ctacccctgc gtgggaggcc tctatgctgt tctccgtctg atcaaggatg acagccagca
    31021 aatcccgacc ttcagtatgc tgggttatca cccggatcag gagtgcaccc tgcgccgtat
    31081 cctgcaacgc cctgaaggca ttatcaccct ctctggcccc accggctcgg gaaaatccac
    31141 cacgctgaga accgcctctg cggcatacct tgaacaatac ggcttcaaca acaccggcgg
    31201 tattcttctg ccacggcgca ggttgttcac gattgaatcc ccgccggaag gccgtatccc
    31261 cggagcaatt cagacagccg ttatggatac cacccggggc tgggtggact ccatcaaatc
    31321 agcactgcgt cttgatcctg acagcatcct gaacggtgaa atccgcgatc acgattccgc
    31381 cattacggca ataaaagccg ccatgaccgg gcatctgatg ctgaccacca tccatgcgaa
    31441 cgacccgatt aacatcctcg aacgccttga gatggagggt gttcaggcca ggatgattgc
    31501 agacccgcag ttgttcatcg gactgctcag tcagcgtctg gttcagctga tatgtcccca
    31561 ctgtcgtcat ccatggaatg acgtagcatc ccgtcgtact gaggatgagc gacatctggt
    31621 ggaaacattc tgtaatgctg atgcggtata tctgagaaac catgaaggct gtccacactg
    31681 ctggcgtggt gtcacagggc gtacagtgat tgcagaagtc atctctcctg acgccagatt
    31741 ttttcagtta taccgggaaa aaggccgtat cgaagctaaa acctactggc acagggaact
    31801 gggcgggatg acgcgtaatc agcatctgct gggaaaaatc aacagcggac tggttgaccc
    31861 tctggcggcc cattacatct ctcccattga tgaggacagc tatacgctgc tgcgctgata
    31921 tgaaatttct ggaaaaactc aacagtactg aactggatct gtctgccctg aacagctgga
    31981 cagccagaaa aaccttcggt cttaagcgtg ccgagttcta tgagtccctc ggcggaatga
    32041 tacgtgacgg aacgccgtca ctgcgcgccc tggagtttat ctgtaatgtc gaaactgact
    32101 ttggcacccg gaaaggaaca tccggcatgt actttctggc cagagaatgt attgcctccc
    32161 tgaaagccag cggtatgctg tcgggggccc tgcccacatg ggttcccccg gaagaagcgg
    32221 cactgatcag gaacggtgaa gaacgcggga acatcgccga agccctgttc caggtcgcaa
    32281 aaaccgtgaa agcacggcgg gaaatggtga gcagtctcat cacactctgt ctttatcccc
    32341 ttctgctgct ttcactctgt gtggtgaata tgtacaacgc acatacccgc atcatcccca
    32401 ttgtcagcgc ttttgcaccg gaggagaaat ggtcattcca gatgaaagtc cttgcagcca
    32461 tgtcagagct tgtcatcggc tatggtctgt ggctcagtgg tgccatcatc acgcttgtca
    32521 tcatcatccg cttctcactc agtcgctata ccggcccggg acgcaggctt cttgacaaac
    32581 tgccaccatg gtcgctctat cagacttttc atggtgtgaa ttatctgttc agcatatcgt
    32641 caatgctgca gataaatatt ccgctttcac aggcgctgct cagaattgaa aaaagtgcag
    32701 agcataataa atggttaaag gagagaattc aggcaataag aaaaaatgta ttatccggcc
    32761 agaatctggc caccgccatg cgcaattctg actatgattt tccttcaaaa atatgcatca
    32821 ataaacttct cttaaattct gaaagggaag acagcgtatc tgcaatgaat acctatgccg
    32881 gacagtggct ggaagatgca aaaaagagcg tcagaaaaag tggattaatt atcaccggta
    32941 tttcaggagg ttttgtattc ttttttattc ttaacatgat tagcgcgata tacagtatca
    33001 gcgatatgct caagcaataa tacctgccgt aacactaaaa cgcccttcaa tatactttca
    33061 ttaacgtaac aaccattaat acaggagtca tatatgaact tcagagcacg tctgtcttct
    33121 tatttttctt taaaaaaaga taaagaagat gataaaggtt taacattatt agaaattgcc
    33181 ggcgcaatga ttatcatcgc aatagttgtc gcgctcgcga tgagccagat gtccagcaca
    33241 atggcctcca gtgacaacca gcgcgaactg aaaaacctgc agacaattgc aaccaaaatg
    33301 aagcaataca agttccaggg aagatatacc ggaacggatt atatcaaaac cctgacggaa
    33361 agcggtgcat tgcctgctga tatgattgca ggaggcaaca aagccaaaaa cgcatggggt
    33421 ggtgatgtca cgattgctgc cacagcagac aaatatggct acattatcac cagtaataat
    33481 gttcctaaaa caaactgtgt tgagctgatt aacagcctgc gcagttcgtc catatttacc
    33541 aaaattatga acacagcccc gaacacggtg gatcccgtta ccgtctgcag tagcgataaa
    33601 aacaatatca agctggaaac caacagctaa tgccctgcca cacagcactc cctccggggt
    33661 gctgtttccc ggagcacata atcatgaaat ggttttatct ctcatctctt ttcttattct
    33721 gtctcccggc aactgccgcc gtaaacagta acacactgga tagctgcttt attcaggcag
    33781 gaaaacgtta ctccatcgat cccggactac tgaaagtaat tgcccgtaaa gaaagcagcc
    33841 tgaatccccg ggccatcaat cataacagaa atgccagcgg aaaaattatc agcacagatt
    33901 atggcctgat gcagattaat tccacacata ttcccggact gaaaagaatg ggaattatcc
    33961 gaagtgaaaa tgaactgctc agtaatgttt gcctgaatat acagacaggt gcatggatcc
    34021 ttgcccggca ttttcagcag tgtggtgtga actgggagtg tctcggtagc tacaacgccg
    34081 gatttaataa aaataacact caccgcagaa tgaaatatgc ccggctgatt tatgccgcat
    34141 atatgcaggg acgttaataa cggagaatac gagccatgtt cctcatcatc ttactcgtga
    34201 tctccgctac gctgaccata cagttacttg ccatcagact ggccggacat tatctcatgc
    34261 aggtgcatac cctccggcgt ccacgtcacc tctccctgct ctgtgctgtt ctggcactca
    34321 ccgccaccat tacgcacatc atacggggga cagacattac cggactttgc tttctgttgc
    34381 tgatggccgg ctgcatgaca ctgatgacac tgactgatgc agcctcctgc cgtcttcccc
    34441 gtcccttcac cctcacattc ctgatgctgg gtgtggcgtt tcgtttatgg cagggcgatg
    34501 ctgctattgc gcttgcctgt gctctgttct ggtttctcct gttatctctc ctgaggcgct
    34561 acacctcata ccgcaaggga gtggagacgc tgggactggg ggacgtgttt ctgattgcag
    34621 gtgtggccat gtggtcatct gccaacagca ctccgtggat catcggtatt gccgcagcca
    34681 gcacaatgat ctgtatgtcg ctgttcccgc gcagtaatac tggggccgct attccgttcg
    34741 gtccgtttat ctgtgcatca ctctacattt tcactttttt tcctggtgcc atttttcaca
    34801 ccggagggta acaccatgca aaaagacgac gataaaggtt ttgttctgtt tgaaattctg
    34861 gccggactga ttgtgatcgg tatcgccaca ccaatgattt attcagaaat tgaaaactgg
    34921 ctgaatgagc agctttacca gagtgctgca tatcatgcag atgcctacaa taccgcagcc
    34981 agaaactata ttgccgacaa caatgcccgt ctgcatagcg gcagccttcc ggcaaatttc
    35041 acggctgatg acctgattcg tcagggatat ctgaaacagg ggtttaacca ttctcccttt
    35101 ggtcagagtt atatcaccgg tatccgccgt aaccagacca ccgggcgtct ggaagccctg
    35161 acctgctcaa ctggagggca gacaatcaaa gaagaagggc tgcgtagtgt ggcgggtcag
    35221 ttgccggggc tgggtggatt tatcagcaaa aatggaacgg caacaggcgc attcggagcc
    35281 tggacagata aaccgggaga ctacggactt acctgttcga caggtcacat cgccgtcgtc
    35341 atgagcggtg atgacctgca ggaaagtgac cgtctctacc gttttcaggt agcaggtcgt
    35401 cctgaactga atcagatgca taccgcaatc aatatgggcg ggaataacat aaacaacacc
    35461 ggcaatatca atggtcagag tgccacactg aaaggtgata ttacgagtga agacggctgg
    35521 ctaatcacca gaaacaataa gggatggatg aatatcacac atggtggtgg attcaccatg
    35581 acggacagcc agtggatacg cgcggttaat ggcaaaggca tcacaaccac cggggaaatc
    35641 aaagggggga aagtcagtgg tggtacagtc cggtctgacg gtcgtctttc aaccggggag
    35701 tatttacagc ttgataaaac agctacggca ggcacaaagt gttcaccgga cggacttgta
    35761 ggccgcacca gtacaggggc catactttcg tgccaatccg gtatgtgggc aggttttgaa
    35821 tcctaccctg tcggaagccc tataccgtgg ccatcagcca cccctccaca aggctacctg
    35881 gtgatgaacg ggcagtcatt ttcatgttcc cggtatcctc agcttgcccg tgcttatccc
    35941 gggtgtaagc tgccggattt gcgcggtgtt tttatccgtg gctgggacaa cggacgtggg
    36001 ctggatggag atcggaacag acatctcctg agttatcagg ccgatcaatc cggtgtctat
    36061 catgagcgtg ggggatggct gaaagggcat cacagcggta tgccttactg ggcccaggga
    36121 agcacaacgg aaatgcgccc caaaaacatt gcctttaact acatcgtcaa agcaagctga
    36181 tgcatttaag gcgtattaac ttttaactta cagcaacgta ttttgaccca gaccatgctt
    36241 cccgaatacc ccggtttacc tctgtacgct ctgaatcctg ccgcaccata gcctgctttg
    36301 ccgtcacaga cggcaaagcc gtttgagcca ctaccccagt tcgcataaac cggttttctg
    36361 gagtcttctt ctgcgtcata acaggccgaa taatcaaaaa ccagtgcatc gctgatatta
    36421 ttcttccaga caccggattg gcacgctggc tactgcctga cgcttaatta aataccggcc
    36481 ccaggtatat tgagacacgt aacagatatc gttccacctg actcataatt accccacgca
    36541 taattattga cataccagac attattttta tcggaataaa cctgctgaaa accaccgaaa
    36601 ggagaaccat taagatacgt ccatgcacaa tatgcatgga caccaagtct gaaattacga
    36661 atattttttc cgatgttata ggtctgcgtt gtgaatttaa tttcagttga ccgccagaca
    36721 ccggattggc acgaaagtat tgcatttctg tatccagcct cctaccttcc ccgtttttat
    36781 cgtggaaggc agatatgttt caaaaaaaaa ctcgcaaaat gtccctcagc cgggcactgg
    36841 acaaatacct gaagactgtt tccgtccata aaaaaggtca tctgcaggag ttttatcgcg
    36901 tcaatgtgat taaacgtcac cctatggccg atcgctatat ggatgaaatc actaccattg
    36961 atatcgccgg ttaccgggat cagcgtttag ctcaaattaa cccacgaaca gggcgtcaaa
    37021 tcaccggaaa taccgtccgt cttgaacttg cccttttatc atcattattc aatatcgccc
    37081 gtgtggaatg gggaacatgc cgtatgaatc ctgttgaact ggtccgaaaa ccgaaaatca
    37141 gtaatggccg cgaccgccga ctgacttctg gtgaagaacg ccgcctttcc cgctatttca
    37201 gggacaaaaa tccgcagctc tacgttatct tccatctggc tctggaaaca gcaatgcgcc
    37261 agggagaaat tctctcatta cgctgggaac acctcgattt gcagcacggc gtggcgcatc
    37321 ttcctgaaac gaaaaatggc tcaccacgcg acgtaccact ctcccggaaa gcacgtaact
    37381 acctgcaaat tctgccacaa cagataaacg gcaatgtgtt cagttacacc tcgtccggtt
    37441 tcaaaagcgc ctggcgtacc gctcttctgg atctgaaaat agaaaatctg cattttcacg
    37501 atctccgcca tgaggccatc agccgtctgt tcgagctcgg cacactgaac gttatggaga
    37561 ttgcagccat ttccggtcac cgctccctga acatgttgaa acggtacaca catctccggg
    37621 catatcacct ggtcagtaaa ctcgatgcaa ggcgtaaaca gaccagcaaa atttcccctt
    37681 attttgttcc ctaccctgca actgtcagaa acagaaacgg agtgttcatt gtgacactgc
    37741 acgatttcga ccttgaaagc cgtgctgaaa cccgggaact ggcaatatct cacgccagcg
    37801 tattgttgct gcgcgcactg gcccaggctg ctcagcgggg tgaacgcgtc cccacaccag
    37861 gcgagctacc cgcaaacatt gatgaacggg tcatgatttg tccgttaaca aacagccagt
    37921 tcatgacaaa agcgaacagt attttcatat cctcctcctg aacaaataat ttttgttcaa
    37981 aattaacgct atgagaccct ttatgaaaca cttatcctcc cgtgcagcca ctgcggataa
    38041 taatcgtatc gttacaattg aagacgttta cacaatcgca ttctcgcaac caattcacac
    38101 cgaatcagcg ttggggcaaa ggtggatgtt gatccatact ggtgaatatg aaagtgccag
    38161 cgatgcaaaa cgcttccttg atacgctcgt caccgggcac tgtgcttgca tggtaacacc
    38221 atcacctgtt ctggatcatg acacctggct gcgtatccgc ctgctgatgg gcgagataag
    38281 ggatgccaaa taaacattca tggccccaac acataaaaat aacgccgggg actctggcag
    38341 aggtaccatt ctttttacat agagccaata tataaacaac ttttctatga taatatcttt
    38401 atcatgttat aaataattgc cccctcagca gaaagggccc aaatagaaaa agcaaaaatc
    38461 cttataaaaa tattctttac aagcgttcct atatacaaac aaactatata aaccagccag
    38521 aacagaatga ctgcaaaaga aacacacacg gcccccataa aaataaatgt catcgaatcc
    38581 catggtttca ccagtacaac cggaaccatg gctgccagaa caataagagc agcattaccc
    38641 aacagcgtct ctctaataat atcccttata atctccagtt tttctttatg ctgtttaatt
    38701 ttcatacgaa cctcgttgat ttcatcccga aaaggaaaag attatataac gaaatacttt
    38761 caatctcctg actccgtctg ctctgcagca taatgccaga ccagcggcat gctaaatcac
    38821 gtcctgtccg aatccttttc attcacgctt aaaacagagt taaaagcact cttgaaacac
    38881 actcgatcat aacgatcgaa ataactcgtt tccgttcagt aaacacgctc tttagactta
    38941 acatatgaac cgcacggagg tgaaatgaaa cccaacagca caggcattgc tgcccgcatg
    39001 atgctctcca ttgacagggc ccggattagc gaagctctgc tcaataaccg ccagttgcag
    39061 tccactcccc tgcagatccg ttatccgcag ggcgtcagag aagcactcgg aataatgagc
    39121 gagcaactct ccctttctgt atccgacctg acgcgtatcc tggtcgaaga tgcattaact
    39181 gaaatgtttc tcccggctga aaacatcgtt caccgtctta tctgccggac agaacacatc
    39241 atgcaggcac atgatgtctg cgtaacaacg atgacaacgt tactggctcc ctggaatatc
    39301 cgtcctgccg ttttccgtga cccggacaaa ctcgcagatt atctgacagg agagattctg
    39361 actgctctgg ctgactggtt ttatctcaac ccggaatggc tgaacggcag ggtacgttat
    39421 cctttaagcc gtgccggtga gtggccagca acacaggatg cgttctgcca gcttatttca
    39481 gccagcgata atgtggatat tattatctgg cacagttttc cttttgccgg tgttcattca
    39541 caggaatatt gcggtgtact gttccgcaag aaaaaagaaa ttaataaagc catcatttat
    39601 cctgtacttt ctttatcacc tgtaagaatg aataaggaaa aagaaaactg gtttcacact
    39661 gcccgcaatt tatatgcaaa gacatccgtc cggaccgtaa cgcttcgtcc ggcacaggca
    39721 gaatatctga tgacaggaaa aatattacct gctgagttat tcagtatacc gttattaccc
    39781 tggtaataac gcagagaaaa aaccaataaa tttttaatgg cacagtcgtg tcataccgtt
    39841 gctgtaccca aaacacctca aaccaggaaa ctgaaatgat gatgaatatg cacattaccc
    39901 gaactctcat cgcatctgct gttttatttt cctttaattc aacagccgca acttcatatt
    39961 ttgaagcccg caatgatgca atggggggaa ccggggtagc ctcctctcac tacggtgtgg
    40021 cacctctggc aaacccggcc ctgctgacaa aagccggtcc ggaagatgac ttcagtcttc
    40081 tgcttccgtc cgttggtggg caattgtccg atccgggtaa tatcagtgac aacgcagaca
    40141 aaatcagtga tgactggaaa gcctttgatc gtgccatcga cagtcagagc aacgtgtcgc
    40201 agactgctgc acgtctgaag acgcgacttc aggacttccg tcacacgcat gctgacgcac
    40261 aggtgggaac atctgctgtg gcgactcttc ccggtgacac cctcgctgcc gctctgatgc
    40321 tgaaaagcta cggtacggtc agtgtcgacg gtaaagtcag tgatgccgac ctgaactatc
    40381 tggaaagcat tgcaaacagt aacggtcagg atgtggataa agacagactg acctctcagg
    40441 cttttgcccg tgccgcactg gtcaccgatg tgggtattgc tctggctact gaactggaaa
    40501 cggccggaca aaagtggtct ctgggcttca caccaaaatt ccagcgtgtt gacctgttta
    40561 actacaacgt gctggttaaa aattacgaca gcagcgcttt caaaggcgac cgttaccaca
    40621 acaccagaaa cggcattaac gctgatatcg gggccagcat ggacctggat gacaactgga
    40681 cactgggtct ggtcgcacag aacctgctcc cccgcagtat tgagacaaag gaagtgaacg
    40741 gtgtcactga aactttcagg atccgcccgc aggtgactgc cggtgtctcc tggcacaacg
    40801 acatgttcac tacagcactg gatgttgacc tgaccccggc cagtggcttc acatcagaca
    40861 gtaaacgtca gtttgctgcc attggtgctg aatttaatgc ctggaaatgg gcacagttac
    40921 gtgcaggcta ccgccagaat ctggccagca atgatggcag tgcgttcacc gccgggctgg
    40981 gtatctcacc atttgatgtc gtccatattg atgtggcggg tatagcaggc acagacaaca
    41041 actacggtgc cgttgcacaa ctgcagttca cattctgatc atctctgccc cggtactgac
    41101 cggggtatcc agacctgcat cctcgcgttt tccgaccatc aaacgacaca gccagacctg
    41161 tagtgctgcc tctgatctgg ctgttatctg acatatcatt ttcgcatgag gaaatgcatg
    41221 tttaacaaaa ataaaacgca tatcgaaaac aaagccttta tggtggaacc gttccgggat
    41281 ccgacagcta acgaatgtaa tgtcgtgata gcaaacgata tttgtatccg ggtagcactg
    41341 tcaccctctg aggtgttcca gccattcagc aaaaacacga accagtaaat cctccccaca
    41401 acgctttctt ctttcactgc tcaggaaaaa tacaccatga accgtatgat acctgcggga
    41461 tgtctcgccg tactctgcct gaacggctgc catctccccc cggataatac agctccgacc
    41521 agaaagtatg gtgctgcctc tgatgtcgcc gtcgcccggc atgcgcagta tcagctatgg
    41581 gggcagggaa tttactctgc agcccttccg gagcgtcagt ccggcggcag tctgaaggct
    41641 cacagcgacc gtgtttcctt tgactgggaa ggcgatgccc tggaattgct caacgaactg
    41701 gcccgtctgc gcggccagca gttcagctac agcggcgtcc gtctcccctt acccgtctca
    41761 ctgcacgtcc gcgacatgac ctttgctgat gtccttcgtc tcactgaagc ccagactgcc
    41821 tggcgggcaa cactctcgca gtcagccggg cgtttacatc tgtcttttat gacgccggag
    41881 aaaaaatgaa aacacacctg tgtctcctgc tgtctgctct cctgcttaca ggctgtgccg
    41941 gacagcatca ccgttcttct gccgtcatga cagaaaacgc accgcccccg gatatccgtg
    42001 catggctgac gccctccgga aaccaggatg atggtatttc agatacccgt cgtcagatgc
    42061 tcacggatgc cggcagaacg ctgggattcc gtggaggaaa agcacagcgc tcatgggaac
    42121 tgattcaggc actgaatgcc cgggaaagta ccctgaatgc cctgtatgat ttccggcctc
    42181 tcatcagcaa ggaaggctgg ctgcctcctg ttatcgatga agcgcaggat gttgcacaca
    42241 tcagaccgga ccagataaga acagcctccc gtgtctggac catcctccgc ccggaacgct
    42301 ttgtcagtaa tccacctgga tggcgtgact ggctgctgaa aggactctca accaccgcca
    42361 caccaggcac tgaaggccgg gttgtgccgg aagaccgggc acaacgcaga ctatgggaaa
    42421 acgccttacg tcagggatgg caggaagggc gcctcaacgc agatctcaca ctggaagcta
    42481 atcagaagcg cctgacccgt gattaccggg gcatgatgct ttacgccctt ctgtggcgtc
    42541 agggcatgat cacacgtcca gatgtcacag agcagatgca gacggttacc ggcaacgggc
    42601 aaaaactcat cactggcgat cgcgtccgcc gcctgaaaac acatgctgaa tttacgctgc
    42661 agaaatcgcg ctggcgccct cttgtcagca ccgaaggaga gaaaaatgat atcaccagat
    42721 gacttcggta ccttcccctt ctcccgtttt acggcagaag agtttcgcca tttttttgcc
    42781 tggtgtgccc gccaccgcgt cagtgatatt gatctgaccg gcggttcacc ggtatccgtc
    42841 agtcgctttg gacggcgggt tcgctcatcc gtaaatcccc ttccgtcctc catcatgatg
    42901 gcactgagtg atgagctgtt tgggcgggag gttcttcccc gggttctgtc aggtcatccc
    42961 gtggatcgta ccattcaggt cagcggagat gcctccggtc gctatggcct gcagcgaggt
    43021 cagcgtgttc gcctgcgctg ccacctgata cagggaacat cggcaacaga agaaaaagcc
    43081 atttccgtca ccatgcgggt gatcccgtca gatattccgg acatcctcac tatgaatatt
    43141 gaaccggatc tgctggatgc catgctcaga aaaaccggcc tgggcttcat ctgcggagaa
    43201 accggctccg ggaaatccac actggcggcg gcactgtatc gccacattct gaaaaatttc
    43261 cccgatcgca aaatagtgac ctatgaagat ccggtggaat acattctggg cggagagaat
    43321 gaccttctgc ctccccatca ggccgaaatc gggcgcgatg tggaaagttt tgctgcagga
    43381 cttcgctctg ccgttcgccg taatccggac atcattggta tcggtgaaat cagggattat
    43441 gaaacagcag atgccgcagt ccaggcgggt aataccggac acttttgtat cggcaccatg
    43501 cacacaaaat cacccggaga aacctttgcc cgcctgctgg gattgtttcc tcctgaaatc
    43561 agggattcga tggctgcagc cacgctttcc ctcgtacagt tcattctggt gcaggtactg
    43621 gtcagaacaa atgacggcgg caggcaggct gtccgggaat acatcgtcat gactgacgaa
    43681 ctgcgcaata cgctctccgg acaatcccat gctacatggg gacattacat tgacgaaatc
    43741 attcgtcagg aaaaacggcg gataagggat caggttctga ccatgtatca gaacgaccgc
    43801 attgagaaat cagaagcagc gctcttcatc ccggccggag agttcccccg atgaaacgcg
    43861 aaggcttctg gcgggatgta acaccaccgc tcaacattta tggtgtcccc ctgccgttta
    43921 ccatgattta cctgctgttg tttccgtatc cgtcaaaaac gacattctgg gtgtgcagcg
    43981 gcattctgct gttttttatg ctcctgggat attccggctg gacagtccgc accctctgta
    44041 tacgtctgta cagcctgctt cagggtaagc aggcatcggg acgtccctgg tggtaccgac
    44101 accgcacaga attaccccgt gaatggcacg gaccagaata gccctctttt gtccatcatc
    44161 ccggcgtatc acaacgccct catatttaat ccccggagaa cgacatgaag tccagatatc
    44221 gtacctggct tgctgtcccg cctgaggaga cagaagccgt aaaaaatgca gtccccccgt
    44281 taaatggccg caaagcggta gcatgggatc cagagaaaaa actctggtat gccgaggcgg
    44341 gtaccgacct ttcactgctg aagcgctggt taccccgtcc acaggaaatg tcgatggatg
    44401 ccggcgatcc ggtcacagaa tttgctcagg tcctggaaaa cgccgggctg gttattcagg
    44461 gattgccaca gatggatggc gccatacaca gagtcgccac ccgggatgac aaaaaagggg
    44521 cccaaagtgg cgcatacaag gcctatcttg atggccgccc ctccggctgg taccgcgatt
    44581 accggagcgc tgataacagt cccacaacct ggacattctc ccgcgcagaa aatatcgatc
    44641 ctctcgccag actgcatctg aaggcatccg ctcagcaaaa tcgcgatgag aaagaacgta
    44701 aactacagca acagtacaac aaacaggccg gctatgcccg ctcctatctt aacgggttgc
    44761 cccaggcaac agcacatgaa tacctgacac gcaagggcat ccgggccgcg cctggcgtgc
    44821 ggctgaacaa taaaaatgag ctggttattc ccttcagcaa cggccggggg gaaatacgtt
    44881 cgtaccagcg tattccggtc accggtggga aggatgcccg cattctgaaa ggctctgaga
    44941 aaagcggcaa ctggttcgcc ttcggaaccc cggaaaatgg ccgtcccctg ttatttgcgg
    45001 aaggttacgc tactgccgcc tccctgcacg aagcaaccgg ccttcctgtt ctgatgacaa
    45061 ttgatgccga caatatgatt aatgtggcaa aaaatgcccg caaaatctgg actgacagtc
    45121 cctttgtctt ctgtgccgac aacgaccatc aacggaaaat taataaaggt gtttcaagtg
    45181 caaaaaaagc tgccgcaagc acgcatggcg aagtcattat tccgaccttt acggaggcgg
    45241 aaaaagcaca gggactgacc gatttcaatg acctggacgc cagccgggga cgagacgcct
    45301 ttcagcgagc catgaacgca cagttagaat atatcggtat ccccacgccc aacaatgaca
    45361 ctactgaccg ccgtgaagct atcgtgatcg gcaacctgat attcacaccg gtaaaagatg
    45421 agaagccaca agtgtcccct gaaaacagac agagcacagc ccctgaaccg gcagtgtcgg
    45481 aacagaatca tccttatgac atccctgcag aagagacaga caatcaggca tatgaagatg
    45541 actatgcagc atatgcatcc ctgcagtcgg taactgcagg gacagcctcc ccgaatgaac
    45601 cgcaggtgaa tgctgtttct gccattgaca gcaacgcaaa gaatacctct gccgccacca
    45661 ctgacgaccg aaatgtatta gcacaagaaa ccaatgctga cgccacggtt acgaccacgg
    45721 ctcacgacac cggcacagca gaaacagatt caccttctga acctgcagag attcagccag
    45781 tgcagcaggc cagtcctgaa ccatcggcta aagataaacc acaggagaac gtagtgtctg
    45841 ctactgataa tgacgcagag aacacctctt ccaccaccac tgccctccgg agttacgaca
    45901 caccggaaac atccgtctcc gacgcaaccg ggacacagca caccgccaca acgcaacctg
    45961 aagaaccaca gcaggtctcc atgggcagcg ctgacgactg gattgcattt gcggaagacc
    46021 tcaatgctga tgctccgggg aatgccactg ttcacagcac cgacaacgcg gagacaaaga
    46081 cactttctga acctgccggg atgcaccctg ttcaacaggc aacccctgaa ccaacggaag
    46141 aaaataaacc tcaggaagag ggcgttatgt ctgccactga tacaggtgaa gagagtgccg
    46201 ctgccgctat cgatgacgac ctgaacacag ttgcggaaga cctcaatgct gatgttactg
    46261 tgatgaccac agctctccac accgatacag cagaaacagc ctcaccttct gaaccagcag
    46321 aaattcagcc cgtacagcag gcaacccctg agccaacagc agaagataaa ccgaaggaag
    46381 agcgtcctgt gtctgccact gagaccagca cagagaatga agcagcccgc cctgaaacac
    46441 catcgctttt cagccgcctg cgccgttttc tgaccggtgg aaaatcacgt accgccgaac
    46501 ctcccgttat tcaggagaac acgcttacgg aggccgaaac tgcgacagag gccgcagcac
    46561 caccgtcacc acctgaaacc agcccggaag acacatcgtc agcagaaccg gacacgatta
    46621 tctttgcgcc ccgccggccc aacagtccgc agcggcacaa cctggacgag attattaaaa
    46681 atcttacata cgatgagtta cccgacaata ctgcgctctg ccgcgtcgac ggcgaaccgg
    46741 cattcctgga taaactcaac cgtctggaaa tggtggatgg tgccagtgct gatgacaaaa
    46801 aggtcctggc tgcccttgct gtggccacca acttctatgg cggcgttatc gagctgaccg
    46861 gcagtgatgc ctttaaacaa aaagccatgc agctgattgt cgaatatgac atcaaggttc
    46921 gcatgaaact cccggtccag agggctgcgc tggaaaaact ccgccaggag aaagggatag
    46981 cgcatgatgc catcgtcacc cacagaccca cgccggaact caaccgcctg tcaccggaag
    47041 agacaacaat ggcacaaccg gccccccctg tgtcaccgaa agcagaaaca gagcaaactg
    47101 caggcagtac ggctgaaaca ccgcatccgg aagcgccggt cacggcaaaa gctgaagccc
    47161 cccgagccgg cacaccggaa caacccgccg cctcgtcgtc tgcgccagaa aaagcccctg
    47221 tcagtgcaca gccgcagcaa cccgacacac cggaggaaga gccgcccggc aagctcagac
    47281 ccggtgaaag tgtgactgcc gtcctgacaa actacggaga aaaagagtat gcgcccggaa
    47341 aagggcgctg cttttttgtg gagctcacca accgcagtgg caaacgggaa tactggggaa
    47401 acggcctgga agaactggtc aaaaaccacc agaaaggcaa ccccgtcaca ctgacgctgc
    47461 aggcgagaga aacattccgg ctgtccggtg aaacacggga tcgcatccgt aatatctggg
    47521 ccatgactcc tgtcagtaac ggcattaccg tcgataatga ccatcctcat gctggtcagc
    47581 atatcctgga atttccgatg aaaacattca gtcaggtaat gtcacaaatg caacaacact
    47641 ggccacagat aatgcaggaa gttcgctccc ccaaaaatcc tcccaataat ctgtacattg
    47701 gtgaagacag gcaacctgca tcagcacctc aggatcaatc aatgcgactc cccctcgctg
    47761 gtaatgcccc caatgaactg ataccagcca tcgcctctgc cgataaggac aatcgtcagt
    47821 tgaatcttct gctggtccac agcgcagatg atcatctgca gggcgtggtc cgcctgaacg
    47881 gcacccttta cccggcactg gccaccccgt cagctgacaa ccgtcagctg gtcatcaatg
    47941 ccctgacgga taacggtctg cagtttgccg gttacggtga agctgttaac catgatgaga
    48001 atactcatca acgtccgtcc ccacaaataa tgcagtttca cctgaagcaa caggactccc
    48061 cgttgtttgc cgccatccac aaaccggaag agcaaccgga caagcttttc cgctctctgg
    48121 gttttgagca gacctggaaa gagtggtctg acagccaaaa ggcggaggac aggcaggaaa
    48181 aaactctgca acaggcacaa tcccacagcc ccggcctgtg agccggtaag gacgaccatg
    48241 aacatgaaaa aaacgctgct ggtcatactg accagcatct cgctggcttc tccggctgcc
    48301 gtggcagcaa cctgtcgggc aggtgctgcc gcagaagccg gcagcaaagc cgcctgggag
    48361 cgggcgcgca cagccagcca ggcatggtca cagcgagaaa acgacgtcgc ctcctccctg
    48421 cagtcctgcc tgtcccgcat taaggacgtc agcatcactc ttccacaatt ccccagcctg
    48481 gacaaaatcc ttcgcgatct ggaaaaccag gtctgtgatg ccgtcgtgga tcgggtgaat
    48541 gaacacctgc cggggaatat cgacccctgg aaagactata acctctgaac aggagcccca
    48601 tgagcgaagc acaccccaca tcccctgtct ctccggaaac agataacagc ggagatattt
    48661 ataaacaaac gctcagtacc attgcacagt cagaacaacg ggccgcactg gttcccggac
    48721 tggtcagggc cttgctgtgt agcagcgcag ctctcgtcat cagtctgacc ggcaatatca
    48781 tacagtactg gcataacagc aacgtaacgc gtgaatactt tgccaccgac aatggccggc
    48841 tggtccgcct ggcccccacc agtcagccag cctggagtca gagcgacgtc atgacgtttg
    48901 gcagcatgac gctgagcgaa gcttttaatc tggattttgt ccactacagg aaacaaatca
    48961 cgacactggc cccccgcttt tccgaagccg gttttgccgg ctatgtgaat gctcttcagg
    49021 cctccaacat tctggacacc attaaaaagg agaaatttaa cctcacggcc accatcgggg
    49081 ccggcacgct ggtgaatcag ggccagatgg aaaacggcat ctggttctgg aaattccagt
    49141 atccggtcag gatgcgtctg gtcggtcaga ccagcacccg ccccgagcag gccttcacct
    49201 ttgaaatcac tgtccggcgc gttgacccac gaatcaaacc cggcggcatg gaaatcagcc
    49261 agatggtttc ccgtaacgcc gcatcaggaa cctgagactc atatcatgca aatacatcat
    49321 cttcttggcg cactgcttat tgtgtgcggc actgtcacgg ctgcagataa cccgggctgg
    49381 cagaacgcac gaaccccaca ggctggcgga aagacaggac aggctcccgg gaacgccccc
    49441 gcaggcggcc ccccgccagc cacacaactc acaggccccc tgccgccacc tgcctctccc
    49501 cttgttcagg aagccgcatc actggattcc cccctgaatg caggtgaaat tcgggaactg
    49561 cggggacgaa tggccgataa cgaacgggcc atcagcgcac ctgtcaccag tgtggtgccc
    49621 cgtatcagct ccctcacggt aaatctgtca cccggggcca gtctgccgct tgtccgaacc
    49681 gcactgcata acgtcagcgt ggtaacgttc acggacatca acggctcccc gtggcctcag
    49741 tcggatccgc cttataacgc ggcacccggt cactttgatg tgcagttcaa cggcaatatg
    49801 gtctccgtca ctcccctgcg cgcctgggct gcagggaatg taacggtcta cctcaaagga
    49861 ctgtctgtac cggtcattct gaatatcacc agtggtgaaa ccgacaccaa taccctgagt
    49921 caggagatcg acagccgtct ggatctgcgc attccccgtc agggacctgc cagtcctgtg
    49981 gtagccgtac catcagagaa gattgccctg catgatgcca ccctgcaggc ctttctggac
    50041 ggtgttcctc ccgatgatcc caccgtgcgt cggctgaaat tctccggaaa tgttccgggc
    50101 accaccatct ggcagcacgg cgatgatctg ctcgtgcgat cgcgggccat gctgcgggat
    50161 gaatttgaac agacgctggc atctgctgac ggtacgcgtc tgtggaaact gccggtgact
    50221 cccttactca ccttctccgt gaatggtcag tccattcacg tcacccctga actggagtaa
    50281 atcatgagcg cagaacagga cacagccgca aagtccggca aaaaactgtc agccctgctg
    50341 gttctcgggg gcgtgattat catcgggggc ggatacctgc tgctcacctg gctttccccc
    50401 gaaaacagcg catcgctctc gtcagtcaat atcaacagca cgacaacagg cagcgggcgg
    50461 accgtggtgg agtcacagca ctaccgggat ttactgcgtg ctgacaatga gcgcggggcc
    50521 gcagaagcgt tacgtaataa ccagacgttc atcgccagtc tgcccagggg actggatact
    50581 gatcacggaa ataatcaaag aagggatact cagcctgctg accaggccga gggttctggt
    50641 caaccatcaa cgccccctca ggcagatcaa acagcccggg aaaaacagcg ggaagcgtta
    50701 caaaaactgc ttgcccgcat caacacacag catcctgacg cccgcccccc ggtagtggcg
    50761 gctgcaatgt ggggtaccac tggtgctccc gggataagct cacacgcctc accacccggt
    50821 accggcacat caccacaacc acgtctgcag gaggccagtc tggcaacccc ggcagctcgt
    50881 acagggatac agctgatacc ggcactgacc cgcgtacctg ccttcattga tacggaagtg
    50941 gactctgaca atgctgtctc ccgcgtggtg gcctccgtac ctgccggccc atgggccggg
    51001 gccgtcctct acagccagga cgccagactc gccggcaagg gaatggatat ccactttgac
    51061 cggatgatgt ggcggggcat gtttctgaaa gtcaatgcat atgccctgaa tgaacatacc
    51121 ggcatgtcat ccgtggccag cgacgtgaac caccgctggt ttaagcatat cgttctgccg
    51181 tctgtgctcg gtggggtggg cagtgtcgga agtctttaca aagacgccaa cacacaggtc
    51241 attcagggaa actacggcag cattaccggc agggtgggaa tgcctgatgg cgaggcgctg
    51301 gctggcgtca ttgcaggcgg catggccgaa cggggcagtc agttgctgac ccgccaggcg
    51361 gagagcgaac cttaccggca ggtgacagtg tcacgcggag aggttgtctc cattctgttt
    51421 gttgagccgg tcatgagcaa cgatatcagt cccccctcct ccggcactgc cccttctgtg
    51481 ccaccgccct ccggacgccc gctcacccag gaagaagcag agcgtcaggc gtctgacagg
    51541 atccaggcag ctgtcatgcg taaacagaac gaaatgcggc aacgttacgg aatacaacag
    51601 gaaagtcatc catgaaaaac gaacatgaag atcccggcag ttttggcgaa acaacgacac
    51661 cggcatccgg caaatccttc ctgaaacgcc agtatatggg atacagcctg ccagtctggg
    51721 ccggctgtct cctcatcatc ctggccgcag tctggtacaa gtttctgcgg gcaccctcag
    51781 agccccctgc agatgcaggc ttcgctgtca caccgcccgc agccgaaagc tgggcagagc
    51841 ccatgcccat gacggagacc gggagcggaa acaccatgtc cgcgcctcct gtcaccccac
    51901 aggcagacat gcagcagctt gcccgtgacc tgaaagcaga gctggacagg cgggatgagg
    51961 aaatccgacg taccctgacc atgctgaaag agagcataac aacccttggc gacaccatac
    52021 ggaaagatga ggcctgggca caggaaaccc gtcagttgct tgatgaaacc cgtaagcagg
    52081 tgcatgccct gcagatgcag ggaccactgc aaatcccgac cgggaataac ggccagtcaa
    52141 cgaaaacccc gccttcccgc ctgcagggca tgcaaatcat gtcggtggaa aaaggaatgg
    52201 cctggatccg ctggcagggt agcgcctggg cagtacggga aggtgacagg ctggggaata
    52261 tcaccatcac ccggattgaa ccgtccagcc gctcagtcat cacctctgcc ggtacattac
    52321 gatgatgaga attgatgccc tgacagccat agagaacttt gccagcggta tattcagtgc
    52381 aggcatggag tttctcttca cctggggaga gctgctgggg attatcggcc tgattggtca
    52441 tctgatgcgt gcccgggctg aagggcgtca cagtatggga ccgggaaaat ttatcgccgg
    52501 tatcgtcatc tgcggcatgc tggtcgccct gccatccttc atcaatgccg gggggaccca
    52561 gatgggattc agggcagatt ccttcggccc tatcgcttat gtacaaccaa ccacctttgg
    52621 tgccgctgcc ggtgccgcca atgccatgct ctcactggta aaactggccg gagtggggtt
    52681 tgccatgaac ggcatttcca tctggcgtaa atccctgctg gacgggcata ccgccctgag
    52741 cagcagtgag tccatcagca aaggaaacgt caagtttgtt gccggcgttc tgctggtttt
    52801 tatcgatcgc gtcatcgatg cctctctggc gagtcttggt ctcgccttct gatccacatt
    52861 cattttcagt aattcagtct gttagttaag gagactatat gtcacgattc cgtgccatgg
    52921 cggcgcgtct gtgcgctgca tttatttatg ttcagttatg cctggtggcc tgccgtgacc
    52981 ggctgatacg cctgttactt ctgcttcccg tgatactgct gccccacaca gcgatggcaa
    53041 acaccctatt cgatatgatc aaaaccgttg gcgatggtgc aaaaggctcc agcagttcat
    53101 ttgtggccat cgctcaggtt attggcctgg tgctctttat tgccggcctg cttggcctca
    53161 aacaaatcgg taaaaaaccc ggagcagggc tggcctgcat actgtccatt gtggtcggtg
    53221 cctgtcttgc cgtcgtaccg gaaatgatga gcagtggaca gaaaacgctc ggtctttctc
    53281 cggtctccat cagttaatcc acaacggcac cggtttcccg gtgccgtctt cacacaatga
    53341 agaggaccag accatgtcct gtgagctgcc ccctttcacc ctgctcagaa cagagcacgg
    53401 caacgtgatt gcccgcgaca accgggataa tcaggagttt gtgtttgcag aatgttattc
    53461 caccgccatc gcagcactga ttctggctgc cgtcaccctg tatacagaga gaaaaacgta
    53521 tgaacaaaac accgccactc atcgccccgg cccgggaacg ctggcttttc ccgctcccgc
    53581 aaccggacac ggtattcagc ggtgaatccc tgctgccacc acccgtgctc tcaacacccg
    53641 gacgctgtcc ctgctgtcgc aggaccgtca ctcaccgctt cattctggag gattcatggc
    53701 cattacagca gatggctgac acctgccggg atacggtcgt cctgctggaa aaaaacctca
    53761 cccgggtgat gcgcctgaaa aaacatccgg taccggaaaa cgctgacgaa aaaaagaaac
    53821 acacccgaac cctgcaggat gcggagcggt ccctggcaca ggcccgcctg tcggccaggc
    53881 gactggccct gcgtcatgtg gaaaagtcac aaatcgtcac aacagatgca ctcagcgaaa
    53941 atgaaagtga actgctgcag cctgaaggcc cgccctttca tctctgtgcc ttctgccacg
    54001 cctggcactg cctgaacggc tacgctgccg cacagggggt gatggtctgg ctccctgatt
    54061 tgcatccggc ctcggtagtt gctctcaatg cacgggcact gaaggaaatt ttcagcgatg
    54121 agcgtaagcg aatcagacag ggacgggccg tactgaatgc cctggtacag aaccgcctgg
    54181 ccgttgagga gaaatttcgc acctggcgtc cggcggattt tgctgatgca ctgcgccgct
    54241 ggccgccggc acagcgtaaa acactgcggg agaaaatgga cggggtggcg ctcattctga
    54301 tgccagattc gtttcctgac aaaaagtacg tcatgtgaca cacaccgctg ccggtgacgt
    54361 tctgccggcc ccaactgaca gaggaacaaa aaatggatac cctcactgcc ggatatcagt
    54421 tttccgcaga tgcattccgg cgctttatta tccggacgct gactgacgat attccgcctg
    54481 acggagatga aaaagagatt gcccggttca ttctccgcca cccggcattt aacggcggct
    54541 ggctgctctt tctggctgca gaaattcatg aaagatcccg tacccataac agcatcaccg
    54601 tcacagtgag cgtcgataac tatctctcat ggatggatat tgccgtgtcc ggtctcccgg
    54661 aattaccgga tatgcccttc tgtgattttt tcattcgccg ccagcagttt atctatccgg
    54721 gtgtaccccc gacaaaatca ggagaagaat gatgaaaatt caccgcatat ccccggagac
    54781 gcttataaca ctcatcctcg cacacctggc cggaaaagct gacagtaccg ccaaagaaga
    54841 gcaccggcta ctccgtcggt tccttcggga tgatgacggc aggctggccg gcatactgct
    54901 gaatattgcc ggaatacttc agttcaacag ggaattaagc gcccggcata attatccggc
    54961 cacacccctg actgaatttt cattaaggaa acggggaaaa caactacatc tctgtctctg
    55021 cagtctccgt ttcttttata tcccccctgt atttatacga aatgaaagaa gaaaatgtat
    55081 tgttgtacat attaataaaa tcacttatcc gccagtaaat tcactacgct gacagcgtgt
    55141 ttccacctta ttacattttt attattaact gtaatcacac agaaatacgc acctgtccct
    55201 caacagatta tttattttct ctgctgtttt ttcatgccac atcccctgct gtggcatcaa
    55261 aaaatatcag accgtgatga ttcatcctta ccggagtgct ccatgccatg aatatcaacc
    55321 gcattatttt tgccattgaa gactgtatca gtaccctgtc acggtattcc gtttccgcat
    55381 tcttccccga ttactgtgat ctgcacaccg tcatcggtct ggaccaacag gaccgcgaac
    55441 gccgcccctg gctgaaagcc ccctacattg ccaccaccaa acaggggtat tacctttccg
    55501 tctttgaaat cagcggtgcc ctgaaagaaa tggatgaaaa cccggacctg acagcccccg
    55561 gcacactgga atcactgatt acagcgctgg cagagcgaat gaacactgcc tataaaaaca
    55621 tcggtcacaa aatcagtttt gttttcgaac gcgatccgga gcgtggaagg aatgaaatcg
    55681 aagtcatgct cttaccccag cgaaaatcca tcgcacgcac ggggatcctg cttcaggacg
    55741 ttctggatga aaaagtcacc acgctcaccc cctggcttgt ccgggaacgc tgctggctga
    55801 ccgtctggag ctctgaagaa ctgctgagcc ggaccgatcg caaggaccac cagacccgta
    55861 tcaggaaact cgctgaacac gctccgccag cccgatttgc gcaggatcca tggcgctgga
    55921 cactttccgc actcaaaata cggcatgacg cctttctgga cacgctggag caggccctga
    55981 cacatgattc tgatggtctg ctggttcgcc tgatggatat tcatgaagtg ggacgggaaa
    56041 tccgccgtca actggagcgc aacagcacac ctgcagtatg gcagcctcat ctgcctgaag
    56101 atgcccgtcc tgccggctgg cgacagggcg aggatacctc tgtattccac gcaccgtcgc
    56161 tgaatctgca gttattcacc acacagcctg aaacacacgg cagcctgata aaggccggtg
    56221 acctctggca cggtatggtc gccattaccc tgccccccca gaatctgcgg acattcaacc
    56281 agctggtcag ggatgtgccc cgcgcaatcc cctggcgtat ccggatggat ctgatgcccg
    56341 gcggcatgaa ggcgcttggg gtcaagaaaa ccctgctgac ctacagcagc tttatttccc
    56401 ctctccggcc catgtatgac tcagtgatga tgctgaatga catcaacaaa catgatccgg
    56461 tctgtatcat gactattgtt gccaccacat ggggaaacag ccgggaagtc tgtacccgca
    56521 accaggcgct cctgaaatct gcgctggaag gctggggagt atgcggcacc accaccacct
    56581 tcggtgatcc gagacgtgcc tgggtgaaca cggttctggc ggcctcccac ggttccggtc
    56641 cagtccccct ttacccgccc ctttcacacg ccctttccct gtttcccctg aacagggccg
    56701 gctctgtctg gcggggacag ggaaatctga tgatgcacac cgaagacggc tctgcatggg
    56761 aggtcgcgct ggcatcgtca caacagaaca agcacacgga actgacgccc ggcgcacccg
    56821 gtctgggcaa atcggtactc atcaacgcac tgtcggagat ccagatagcc tcagcccaga
    56881 agaacctgcc tttcattgcc tatatcgaca agggattcag tgcacaggga ctggtacagc
    56941 ttatccgtga cagcctgccg gagcagagaa aggacgaagc cgtggggatc attctgagca
    57001 atgatccgga acacacccgt aacctgtttg atgtgatgta cggtgctaaa aaacccgcca
    57061 ccccggaaaa gaacttcatg gtatctgtac tctgcgcact gtgcgtggat accggcaccg
    57121 gtcagccctg taatcccggt gacacccggc aaatcataag caacctggtg gatctggcct
    57181 tcagggagta tggcgaaaac aaccccagac tgtaccgtgc aggaacagag ccccttgtgg
    57241 atctcgccct ggaagagagt ggcatcgcag aacaacatga cgctggatgg tggaatgctg
    57301 ccacctggtt tgaaatcagg gacatgctgc atatggctgg caatatcccc gcagcccagc
    57361 gggcacacta ccaggccatg cccctgctgg ctgaaatgtc cgccctgctg ggacaaccat
    57421 ccatccggga tgtgttcggc acagttcagc gcgataactc agcagaattg ttactggact
    57481 acatccgccg tgccctcgat cagggacaca gtgactaccc gatgatgtcc ggctgtaccc
    57541 ggtttatgct cagccccgac acccgcgttg tggccgttga tctgaacaat gtggccggtg
    57601 acaagacacc tgccgggcgt ctgcgcactg gcatcatgta cctgctggca ggacaaatcg
    57661 ccggcgggga ttttgtcctg ccgcagtatc aggaggaaat acggaaaaat ctcgacccac
    57721 gctaccatga ggtgattttc cgccgcattg aacagcttga tcaggaggtc aagaccaaag
    57781 tctatgacga actgcacaat gcaaaaggga tcaatttcat ctgggaagcc ctggacaccc
    57841 aggagctgga gcagcgtaag ttcgggatac gcacagtgct gagcacccag ctattgcagg
    57901 actatccacc ggcagttctc aaatccgcca acaccctctg gttgctgcgt taccgccctg
    57961 acgaaattcc gtttctgcgg gataattttg gtgtccctga agtcaccctg cggcgcttcc
    58021 tgaaaatgcc ggaaggggct gcaccggatg gcagcggtgt cccggtactg gctgtgttcc
    58081 gtgtaaaaaa cggtacgctg gcccggatcc tgaaattcac tctcggtcca ctggagctgt
    58141 gggcactgaa ttcctcaccc aaagacagcg ccctgagacg tgcgctgacg caggagctcg
    58201 ggtcactgcg ggcacgccag atcctggcag aaaactttcc ccgtggttca gccacctcac
    58261 tgatagaaca ccgcgcccgt acacatgaca gtgaaaacgt catccatgat ctggctgcag
    58321 aactcatccg taagcaggga tatcacctct gatgtacagc atacacatca ctccccgctg
    58381 ccggcgtcct gcacggggac aggtcttcct gaaaacgctg cgcgtggacg gtgacagttt
    58441 cagcttcacc ctgccgtccg cctgcttcac gctgcgtccc tcccccaatc cccgggtcat
    58501 tttcgccagt gacagacggg ctgacgggga aatgagtggc gttcgcctgg ctctgcccgg
    58561 agagacaggc acgctgatta caacggccac ctggcaggtc aacgtgccgg agcggcctcc
    58621 ttttgatata cagcatgtca ttcactggct cctgccctcg cctctcacgg cgctgatgtc
    58681 cgacaacacc tggctgtggc aggatagccc cggcacaccg gcactgacgg cgggagactg
    58741 gccggtaatg gaaaccgata ccccgacgct gaaacgcgtg aagcgtcgta ccttcacgct
    58801 catcgataac cgcaaccgtc agggatggat cacccgtcgc ttccagtgct tcccgctggc
    58861 tccgctgccg gaatcacaca gtcaccgcgt accagacatc ctgtatcccc tgaaaacgga
    58921 gaaaaaagaa tgcaaagaca accagcaacc acactgcttg cactcctgat aggctgtgcg
    58981 gttatcccgt ccacccacgc ctattctgtg accgtagtgg ccagccagcc tgtcaccaca
    59041 caaatcatgc cggagctggc cgccattcag ggcgtgatgg cagagatggt ccgtatgcag
    59101 acagccaccg gcacagccat caaccagagt tcagagaaac tggcctctgt cattgcgcag
    59161 gatgggcagg ccacccgtca gcagatgata ttcagtaatg aaacccgccg gcttgaggaa
    59221 gcccggaaga gcttcagcgt gccggactcc atctgcagtg aatccgcctc cggagttgca
    59281 gctgaaagtc gccgtgcagc agcgtctgcg gctgcacgtc tgagtcaggg aggaggcgtc
    59341 agcagtaaac caatccgtga acgtctctcc cgggcagcag actcaccaat ccgcgaggca
    59401 tatgacagtg caggcattca tgccggttac tgcacggaag ctgaatatgc ccgctttggc
    59461 ggaaccgatg tctgccctgc cgtgggcgag ctgccgggcg gagacagcca ggtgcgatcg
    59521 ctctatcagg gtgcaggcac agcagatacc cctgccgccc tcacctggga tcagaaacag
    59581 attgatgctg ccacggctta catgaaaaac accgccagac cgtcagcggg tcgtgccccg
    59641 gggaaaggtg aagtgggaac acagaccggg cgcacgtatg tgggcctgca gaatgaatac
    59701 aacggcatca tcgatgccgc ttctcatccg caactgtcac tgatagctga cagtacccct
    59761 aatgaggcca cccgtggagc gctgactgaa gccctgcagt caccatccgc tgcggcatat
    59821 tttgaccgga ccgcatcatc agaggcccgc acccgtggtc acatgagcca gcgggagttt
    59881 gaggcctttg aagctggcag acgctatgca aacaccgact ggcagcagga tctgcagggc
    59941 atggaaggcg ataaccttct ccgtgaactg ctgcgcacta ccgccctgct gaactggcag
    60001 atgaacgacc tgaaagagca gatacgccag ggtaatgtta tcgccgggca gcagctggca
    60061 ctggcggcac gacagtatta cggacagcgt ctcggtgaac tgtcacaggc catgagccag
    60121 gggagcgtaa gatgaaagac gacaacagca ccgacaaaaa aaaaccggcc ccccgtctcc
    60181 gccgcatcgg tgacatcggg ataaacctgt tcgcaccact ggcagaaacc cggtggatgc
    60241 tgcgcagctg ccgccatatg ctgggccagc ataaaacgcg cctgaagcgc ctcatgcccg
    60301 accggcaacc ggagagtccg gtctgtatga cctgggaaga agccgtcagg gccagcggcc
    60361 ttacggtcaa cgaactggat cggcgtctgc gacgtcgcag gatgttctgg cggacaggat
    60421 tctggctgat gatgataccg gtacttctgc tgtgcggcat ggcccttgct gccacagcgc
    60481 ttccggccat caccctgata cgtatactgg cctgcctgtt actgatgagt gccattgcag
    60541 cactgtgtgc ctcaaaaacc ctgattgtgt cataccgcct ctggcaactg aatgaacggc
    60601 gggtcagcga ggacgaacag ggcaccttcc ccgattacct gcaggcccgg cacggctgga
    60661 aacaggccac atggctggcc attacccacc ggcagtactg accgtccggt ctgcaattcc
    60721 ttttcctcat tccggaggct gatgccctgc atcccttcgg ttccggagtt atcgtgaaaa
    60781 tcatacccag agcactgagt gcgggtctgg cgctggctgc cgtacctgca caggcattcg
    60841 tcacctatca ggacattgtg agcgccgcca ccaatccgga tgacctgtcg cgccaggcac
    60901 tggtcaccat ttttggcgac gtggtcacaa atcccctgac agccagcagc aacaccatta
    60961 tcggtaacct gttcatggtg tttaacgcaa ttatcgccat tatggggata gtctggttcc
    61021 tgtttattgg aatccgccat gttgtccgca ccggccacca gggcaatgtc tttaacaacg
    61081 gacgggacgt cgtcggaatg ctcagtattg tgagcggttt tatgatgatt gtgcccaccg
    61141 cttccggctg gagcctggca caactggtca tgctctgggg cgcatccatc atgggcattg
    61201 gttccgccaa tatcatggtt cagactgctg cagaaaacat cgctgaaggc tattcaatga
    61261 ccgtacagcc agttcatgtc tccacccgca cggcagcacg gggaatactg gagatggcgc
    61321 tgtgcaaaca cgccatgaat gcaggcctcg atgactttaa ccgtactgcc cgttcatcaa
    61381 cgccccccat gagcgaatcc tcacggacag agaacggcag gtataccgtc accgtttcaa
    61441 atggcagtgc cacctgcggg agtgtgagtc tcaccgtgaa agggaatggc agcaacaacc
    61501 agtccgcact ggactatttt ttcaatccct tcattgaaac ggagtatcag ggtgtcctga
    61561 cggcacaacg ctccgccatg gataccatgc tccgtgacct ggacagcgca gcacaggaat
    61621 ttgtgtctgc gtaccggacc cgacgcgaca cgggtaacgg caacctgccg gatattgaaa
    61681 gccgtattca gaaggctgcc aaccggtatg aagaaacggt gcagaaggcg ttgccaacag
    61741 ataacgcaga aactgagcgc agggagcagc tgaaatacta cctgaccacc tacggctggg
    61801 ttgccctggg ggcctggtac cagacctttg ccaccgccaa ccagcgactg gcagaactgg
    61861 ccgaccgtgc ccccactgtg agtggagttt catcgcaggg cgaagccgga gatactgaat
    61921 tcctgaagga agtaaaagga gcttaccagg cacagttgca aaactcacca tatacacctg
    61981 cactgggaac aaccgcttca tcaactgaat acagaacagc agaagctgct gaacctaaaa
    62041 gcataatcat gaaagtatgg tcgggtgtaa attttgcaga caaaattgca acatccttat
    62101 ctggaacagg aaccacctca gaccaactaa atcctctgat aaaaatgaaa aatatcggtg
    62161 attacactat ggtcacaaca gaaaccatct gggcgggtta taccaccgtc agggtacttt
    62221 ccactgccgg cagcaactct atttttggtc gactggtcaa tgccgttagc ggcttatctg
    62281 atgccgcaaa ggcttttctg gaagcagctg caccaccgct ctacttcctg ctgtttttgc
    62341 tgttctgtgc cggtttttcg ttatccattt acctgccgtt tattccgttt atcttctgga
    62401 tgaccggcat cggtaactgg atagtcagtg tactgattgg atgcgccgcc ggtcccctgt
    62461 gggctgccac ccatctgggg acaaaccagg accggggaag ccgggctgct tacggttaca
    62521 tctacctgat agacagcatg atcagaccac tgttgatggt gttcgggttc ttttttgcat
    62581 cggtcgccat cgtggctggc ggcaccatac tgaatgcgtt gtttggcgcg gctctggtta
    62641 atgtgcaggc tgactccctt acagggttat tcagtatcgc gggatttttg atgatttatg
    62701 cccgtgcctg taccacgctg gttgcggccg tttttgcgct tcaggcttat ctgcctgacc
    62761 atgtaattaa ttttctgggc ggacgtgacg gcgttaatat gctggggaat atggtttcat
    62821 ccgtcaaaga tattattgcc ggtggcaaca ccaatatacg cagaacacct ggcgttcgtg
    62881 aagaaaaact gaaaaacatt aatggagatg ataaagacgg aataaaacaa taaagtacag
    62941 acatcaaaaa aaacagtatt gccacattca cctgaagaga ttaaaataca ttcaggtgaa
    63001 tatggaggtt tattatgaac acaacaaaaa gcagatatcc ttcctggtgg gcatattatc
    63061 aactagcccg caatacttac tttatatttg gaattccatg cttattattc tatgcttttt
    63121 tcggaacact aattgttcat gactcaagct tatctcaaac aagagatttt atttttgcat
    63181 atggatgctg ggcattaata attctcccat tactctggct atatctgaaa accagagcga
    63241 agaaaaagaa agtccagcag gttgttcagc agataaaaga aagcagtccc tttgcaccga
    63301 ccaacgatta cgagcaactg tcattctcga agagttgtta tttcgggatc gacattaaaa
    63361 acggcaccat gctgtatgtg cgcatttacc caaacaatgt catggatgtg attggcctgg
    63421 atattcacaa cttcacccga acagttgctg aagacggcaa gctggaaatc cacaccacct
    63481 atgtgagtct gccgatgatc cctcttgaaa tctccggcat ctcagcaagg acgctggcca
    63541 acaccataca caccatggct gcacgtggat atgagtacaa agagcgtttc ccgcagatga
    63601 tccggtatcg ggttaaagag tgggaaaaag ttgccggtgt tcctgttgca gaagtatttt
    63661 gaggtagcta tcaactgtta gacgataatc cgtcagccgg ttttctctga accggctttt
    63721 tcttttcagg cagaatgcct gtctgccgtc cctgtatcag actgtaatta gcacaatacg
    63781 gcaatttgcc gttcagtata ttcaggaagt acaaatcatg ccatctgcaa cacaaactgc
    63841 ccgactggaa gcaaggatca gcacagaact gcatgctatg ctgaaacgcg ctgcagaaat
    63901 ccaggtaaaa aagggcgcat tgaggtttgc tttcgcaaca atccggcggc agatttcctg
    63961 ccgccgtgtt gcccattaac gtttaacttc gtaggctaac gttgccacaa gcactgtgtt
    64021 tccctgctcg acacggaatc cgcaaagcga atccctcacc atccagacaa aacacagtat
    64081 cgtcacacag acgacaatta acattgttaa aaacgttcgt tgtggcatcg cagcccctcc
    64141 attgcctttc ggccgtcatc gggctactat aatgttgtct aggcattaca gtgggccctg
    64201 ggttgattga taaaaaaatc gcctggggct ttctgctgca tgccgtccac aaaacgcttt
    64261 cagcatacag cctcaagcgc ccgccgctat tctaatcgat tttcagtctg ccacaatcgt
    64321 tttccaccta atgacatgtt atgcacaaca gtatttttac gggaaactac acggcaagga
    64381 ttcccggaac acgttcatta tttgatggtt acagacatca tgttaaatcc ggaagtaata
    64441 atgacctgaa aggaaatact atgagtaacg aatatcaaag caccagtata aaccctgatg
    64501 tttttgaaca atttcttacc ctcctgaata cacactgtaa atcatcagaa gctgcacttg
    64561 ccatgctgag tacactctgc tacgaactga tgaggaggag tgggatccat tcactggaca
    64621 gcctgatgct caacggagaa ggtatattga tacaattaca gggggctgat attcgcgaaa
    64681 aaacaggtca gcatattcac tgatccaggg atgtgctgaa aacagtgtca gcctgaaata
    64741 aacgttcaat gcctcataca catgaggcat tgagcacggt catcaataat ctttattatt
    64801 tttctgattc agcatttccg gtcagtatcg gctcttctgg caaagacatt accggaacgt
    64861 cacgttcacc attgaataca gaaacacttt cagcgaaatc attctgtaac aaagaaaaac
    64921 ttaacggcag atttttggtt tgtatccctg tgatccattg aaaaatctcg ggaaaacgac
    64981 gtgcactgat atgcacatga ttgaggtggt gctcagtaat atcactggcc atcatcatga
    65041 gaggtacatc gtatcccgaa cgcaaacctg gcgcatgatg cacaggggtg ctgtctgaac
    65101 tgacggattg cccatgatca gaaaaataca gcatggcaaa atcatcacca tgctctgtcg
    65161 tcatgttata aattgcctga agaaaccgat ccagtttaag cagtgttgat aaataacagg
    65221 atactttatg tccgaataat tccctgaatg gctgtgcatc attaaaaagt cggtcacagg
    65281 gattagtgtg agaccccagc atatgaataa aaatcacttt tggagaagac gagttaacag
    65341 cttccctgac atactgaagc atatcctcat catcaatgcg aagggaagaa gagctccctt
    65401 ccttaagaaa atgtttttta tccgcattgg cggcgatgac agaaatcagc gtatcatgtt
    65461 gccctaaaga gccctgattt gatatccacc acgtctcata cccggctttt ctggcaagcg
    65521 taacaatatt attacccgga ttacctgttt cagtgtaatc aagcgtcagt gttctgctga
    65581 gtgaacctat cgtattcggc gctgctgaga aatacccatt aataaatata cccggagcag
    65641 aattcagcca tggtgttgtc ggcaaaggat acccataaac agaaagataa tcccgtctga
    65701 cactttcacc aattacgata attatggttt tatatttctt ctccacactc acaatatccc
    65761 atgagtcttt ctgattgttt ttcagcgatt catatcgcat catttcttct ctggtttcag
    65821 ctatccctgt cattacattc gtaaccatac gttttggcca tgcatttact gacaatacga
    65881 taaagaacaa cagaaagata aatccccggt ggcttttatc acgcttacgc cagaaccaga
    65941 atgtaaaagc agcaatgacc agcgaaacaa taaaataaag aacaggtatg cctgtgataa
    66001 actcacctgc ctcatttata tttgtctgca ttacagacgc aataaaactg ttgtttattt
    66061 tcccgtaaat catcccaacc ggagcataga atgcacttaa caaaagaaag atacagacac
    66121 acgttgctga aataacattc cgccgcccca acaacatcat caggaacaat ataagcacat
    66181 tccccagggc gtttttcact gtatatccgc agcaccatgt aacaagtaca gaaaacaaca
    66241 gataaatcag tgactcttta caccacaaca taaaataaaa ctgttttgct ctctcaaaag
    66301 catatttcga cttttcttta atacaagaca cgccagtcat tttcctgtac aaacacagac
    66361 gatcatttca acgcatggat atttatatga aacagttcac aaaaataccc taccctgtcc
    66421 agcagggttt acatattatc aggttctgtg tttacagcaa ctgctccccg aaccagatta
    66481 ttcgcataac ccgatgaagg cctcataacc atcaaacact cagaatacat ttagttaaca
    66541 aaaatatgtc ttgccctccc ggaaaggcaa acatacgaaa gagaaagcat ggctataata
    66601 atatcaatca aaatatgctc aatctttccg gttgccatac actcagcacc gacaacataa
    66661 agacaataaa caaaagtgat agcatagtgt gatatataaa gaatataaat gcacagaagt
    66721 aatttcccga caactctttt ccgtttaaag aaagaataaa tgaagaacaa acataaaacc
    66781 gccagaaaaa gaaatgtagc tatagatatt ataaaatact ccgtcacctg tgaattctca
    66841 tccagagatg aatacagcga ataatatgtg gttgtgagat taagccttgt tatcaatata
    66901 taaataactg ataatagtat aatataaaga atcagaccaa atctgttttt ctgcgctccg
    66961 gttaatactt ctgatttatc tcgcataaaa tatcctgtct gtaatctgtc ccggtaaaca
    67021 atggtaaatt tcagaatcag tgcgaatacg gtaacaaaga ttcctcctgt gctttaaagt
    67081 gtgtaggcag aaaccagtct ggtggttccg gtcagggagt ctgatacctc aacttcccgg
    67141 gtgactataa ccatacctga ctgacgcagt ctctgcacat cagctgcaat tcgctgagtt
    67201 cccagccaga acaggccatc cagtgccgtt accagcaacc cgctttccag cgccagtttc
    67261 agccgtgccg tcggggcttt aacctgcccg gcgatatcag taaaacaacg cgtcgtcatg
    67321 ccatagcagt ccatccgccg tattcgcgtc gcaagactgt agctgaaaca atccggcacg
    67381 ccggataagt cattctgcag actgtataca catccccgct gccgcccgtt gaacagggct
    67441 tttttctgac tgagcagcca ttcccgacgc agagtgtcaa tcatctgcgg tgtccgggtt
    67501 aacagcagac gaaacggcag ctcaaaactg gtcacataat caacattcag taatgctgtc
    67561 cgcagtcgct ccctggcacc tgccccgctc tcccggcatt cacgcatgac atcagcccac
    67621 tgacgcatca gtcgtggaga ctcattcctg gccctgaact gatatttctc aatatgataa
    67681 tcaatacgtt cctgcatatc gccaccggat atctgttgtt tacccagaca cagtgattag
    67741 cctacaggaa taaatatcct ccgtgaagcc aggtgcctca gaagataaaa acatgtactt
    67801 ttttcaacgt gtaatattaa caccacctcc acaggaaaat ataaaatgat aaaatatata
    67861 ttcgcactca cacttctgct ttccgggtct gtctgtgccg atcaaattca gggactcgtt
    67921 attcgtgtgc tggacggaga tactctggag atattacagg cccggcatcc tgtgaggatt
    67981 cgtcttgtta atattgatgc accggaaaag aaacagcctt ttgggcgctg gtcaatgaat
    68041 caacttaaaa gccttgtggt aaatacgccc gtaaccgtga catacacaca gacagaccgc
    68101 tacgggcgta ttctgggacg ggtatatacc gaagacggca tggaagttaa ccgctggctg
    68161 gtacagcatg gtgcagcctg ggtatatcct gatttcaaca cagattacac cctgccggca
    68221 taccagagag aagccagaac aatgaaacgg ggtctgtggg cagacaaaaa acctgttcca
    68281 ccatggctgt ggcgaaaaga aaataaaaat agccacaata attataaata agacatctca
    68341 cagaaatata tttcctgccg ttttatttcc cctgccagtc cccaacggga aataacccac
    68401 agaaattaca cagatgtgaa taataaacct gttcgatgta tccgtcacaa aagagttgat
    68461 gtttgactca gaagaaaata cgcctgaagt taaggaaggc ttcagcttca tgattgttct
    68521 gacatgaaca tatatccctc tgtagcgccc tgcctttcat aacatttcta ttacagcaac
    68581 aacgaaagac aacagcaatg tcggcctttt acgtccacat gaatattcca cttatccgga
    68641 attgtgacac agcccctgaa tacaggattg attgccagtg cccacaaatg gcacattttc
    68701 agtaagattc tgaatgttac attcccccgt catgtgtggc acaactgtca ccgggaaaga
    68761 aacggaaagc acatgaaaaa catcaaaact cacaccggac tccttatcca caaagaacaa
    68821 acgcggcgcg ttcgtcttca tgaaactccg gccgcctggt gccacacgca ccgggaatgc
    68881 tacagcaaaa cgaccggtcg tcgctgcggc agccctgaga gtctcagcag gctgattctg
    68941 agcagcattc agcccataga atgagatgac ccgtatgaat gatggcagac agacgcctcc
    69001 gtcccctgag gcatggaaaa tccggtttct ggcgaacggt acacccggca catggtttta
    69061 cacggatgag cagccgtcac aaaagagtgg ctgcgggcac attgtgacga tgtggaagtg
    69121 atccccctgt gccgtatgca tgacggtccc gttttccggg aacaccatat taatgagctc
    69181 agcgccgcac tggccagaat gctccatatg caccacatac tgctggaacg aacccggtac
    69241 agagaaaata tccatgatgc agaatgtatt cgtgaaattc acgaagctcc cctgcaggct
    69301 atcggcatcc tggaaaaata caaacgcaca aaatcataag gcagttctat gtggatactg
    69361 attttctggc tctcatcccc ctttaatgtc tcctccggcg atgcccggca acccacactg
    69421 gccgttcaca gtcaggagtt ccgtactgaa aaagcctgtc gggatgcctt caccgcgctg
    69481 aaaaaagaaa acgtgggaga attcgctctc cggggcgtct gcgtccccgg cggagaataa
    69541 accggagatg atgattagcc acaggagtat tcaatggcat accattcccc ggatatcaca
    69601 ctgcattatc cgtatctgcg ccggatgtcc tgggcgcaga taacggcagc agctgaagat
    69661 gcggaaatac atcatgatta tgcccgggca ttaatattgt ggcagcatgc atatcatgct
    69721 gccacgctga ccctcaacaa aaacctggca gttgctaaaa tcgacttctg tattaaacgt
    69781 atccggatgc gtgaacaaat gagcgggata atccggaaga ctggcacaga cgaaatactc
    69841 ttccgtctga gtaagcacca tcatttatat gagaaaaaga aaacaaaaga gggatgacaa
    69901 tcccatgata tgggaaaaac ttgccatgct tactgcaggt gcatttttga tactggcagt
    69961 cctgtggggg taataatatc ctgtttatta aagggcagaa ggcggtgccc ctctgccctt
    70021 aagtacgttt ccgggattat cagtcaataa gttcccggat cagttccagg attgctatca
    70081 ctgcgttagc cacagcaaca accgccctga gaacgatgat taacaggtta atcatccgtt
    70141 tctccgtcaa tggaggaaat cactgttgtt tactcttaca ttgcctgtca caacagctct
    70201 ttcattgtta gatgtaaagt gatttctctc cagccagagc actgaacaca gcaccacctg
    70261 ttcgatcagc caggacttaa aatctcgcgt tcatcgtccc attggagatc acggcagaag
    70321 catcaccaga cagagctaac ggagcgatta tagtcatttt tctgattgca aaaaagagaa
    70381 gcgcagccta ctatgatggt gttagatgta gagtgatttc cctttcggtc agagtcacca
    70441 actccgcccc ggaatagcaa aaaaaccgac ctcaccagtc ggtttttttg tgcctgtctc
    70501 tcactgcctt ctgctgttcc tgtgaaaata ccctggttat ttcatccctg cccggtcatt
    70561 accggcgatt gttcgcatta attccactca gcctcatccc gaaatttatc catttatcga
    70621 tggaggaata ctcgtgtctt accaacactc acccacaact gaagacccga tgaatatatg
    70681 gtatatcgtt ggcaccatat gcctgctctt tgcgtttctc atctggctct ttttacccga
    70741 catcgtcttt gccacctgcc tgatcctgca cacaatgtgg gggatgactg actgggggcc
    70801 ttttcaccat tttgccgccc cccgttataa cctgctggcc aacaccgcca acaacgctgc
    70861 aacgatcact ttcagccagt ggctggatgt catgtcacag accgtgggga tcctctggct
    70921 gattctgctt cccatgacgc tcggctttct gtggatgtgg tttcagcatc cggcccagcc
    70981 ccgcttcacc cgcagaccac tgaatatcca cacgctgcca cacctgtttt catcactgtc
    71041 accggcgatc gcccccgttc tggctgacgg agacagcaac agactcttcc acggacaaaa
    71101 acgtcccgag cgccgtgtcg cactaacccc cgaagtcttt gtggagcaga acaaccttat
    71161 ccgcaacatg cagctcgatg ttgcgtccac acgccagtgc ttcatggcgc aactggggca
    71221 accactgacc agctggaaag acatggcccc acatgaaaaa gcgctctttg ccatctttgg
    71281 cctgcagttc ttcctcggtg accggaaagc agcggtagcc ctgatgaaca acctcaatct
    71341 ctcctgcagg ctcaaaagta aacgggacca ggggcgtttc agcacgccgg tgtattcact
    71401 ggccagaaac gcctttatcc gggtcatcaa aacagaaggc gcacaacagt ggctcaggca
    71461 gcaccgttac gttcgcagcg gtctggtctg gctttacgca catgatttgc gcctgacgcc
    71521 tccgaactgg ctgtggctca agggcgttga ccggacgctc ttctatgccc ttcaccgggc
    71581 aaacaccaca aaaggcttca tcgaaggggc cggcgtggtg gccgttgccc gggcagaaaa
    71641 tgaagccagc cgtctcggcc tcccctgccc ggaaccctgt gtggaagagg ccattgaagg
    71701 gcttcgacgg gacatgctgg ggctgggcct gatatgggac gaacctcagc cagatcgcga
    71761 tcgtaaacgt cagatcagga cccgctggtc actcacggat gacgtgatac cccgccgcca
    71821 tgataacgac gaagacactg acactgg
//
LOCUS       DAZGQL010000011        69811 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.011, whole genome shotgun sequence.
ACCESSION   DAZGQL010000011 DAZGQL010000000
VERSION     DAZGQL010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 69811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 69811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..69811
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.011"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            178..801
                     /gene="clpP"
                     /locus_tag="ACHKIR_000870"
     CDS             178..801
                     /gene="clpP"
                     /locus_tag="ACHKIR_000870"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFO9988653.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            927..2201
                     /gene="clpX"
                     /locus_tag="ACHKIR_000871"
     CDS             927..2201
                     /gene="clpX"
                     /locus_tag="ACHKIR_000871"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HFO9988654.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            2389..4743
                     /gene="lon"
                     /locus_tag="ACHKIR_000872"
     CDS             2389..4743
                     /gene="lon"
                     /locus_tag="ACHKIR_000872"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HFO9988655.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            4934..5224
                     /gene="hupB"
                     /locus_tag="ACHKIR_000873"
     CDS             4934..5224
                     /gene="hupB"
                     /locus_tag="ACHKIR_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013356995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-beta"
                     /protein_id="HFO9988656.1"
                     /translation="MKRKRRVNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKE
                     GDDVALVGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            5416..7287
                     /gene="ppiD"
                     /locus_tag="ACHKIR_000874"
     CDS             5416..7287
                     /gene="ppiD"
                     /locus_tag="ACHKIR_000874"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFO9988657.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVVQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKK
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            7438..7809
                     /gene="ybaV"
                     /locus_tag="ACHKIR_000875"
     CDS             7438..7809
                     /gene="ybaV"
                     /locus_tag="ACHKIR_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFO9988658.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAAPAAKPTTVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            7903..8301
                     /gene="fadM"
                     /locus_tag="ACHKIR_000876"
     CDS             7903..8301
                     /gene="fadM"
                     /locus_tag="ACHKIR_000876"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HFO9988659.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(8353..9048)
                     /gene="queC"
                     /locus_tag="ACHKIR_000877"
     CDS             complement(8353..9048)
                     /gene="queC"
                     /locus_tag="ACHKIR_000877"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HFO9988660.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLHHYLADKP
                     TVMAAMKQKTGLK"
     gene            complement(9113..10813)
                     /gene="ybaE"
                     /locus_tag="ACHKIR_000878"
     CDS             complement(9113..10813)
                     /gene="ybaE"
                     /locus_tag="ACHKIR_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HFO9988661.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHWETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLLLLQLPARDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPQWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            10913..11731
                     /gene="cof"
                     /locus_tag="ACHKIR_000879"
     CDS             10913..11731
                     /gene="cof"
                     /locus_tag="ACHKIR_000879"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HFO9988662.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGNVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            11884..12342
                     /gene="decR"
                     /locus_tag="ACHKIR_000880"
     CDS             11884..12342
                     /gene="decR"
                     /locus_tag="ACHKIR_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HFO9988663.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            12372..14144
                     /gene="mdlA"
                     /locus_tag="ACHKIR_000881"
     CDS             12372..14144
                     /gene="mdlA"
                     /locus_tag="ACHKIR_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFO9988664.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGQILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAEDAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIRQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDAPEIREEAIDA"
     gene            14137..15918
                     /gene="mdlB"
                     /locus_tag="ACHKIR_000882"
     CDS             14137..15918
                     /gene="mdlB"
                     /locus_tag="ACHKIR_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFO9988665.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVIMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDR
                     PLQSGTIDVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSTLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     EIVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDANTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            16099..16437
                     /gene="glnK"
                     /locus_tag="ACHKIR_000883"
     CDS             16099..16437
                     /gene="glnK"
                     /locus_tag="ACHKIR_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFO9988666.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            16467..17753
                     /gene="amtB"
                     /locus_tag="ACHKIR_000884"
     CDS             16467..17753
                     /gene="amtB"
                     /locus_tag="ACHKIR_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HFO9988667.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNING
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(17802..18662)
                     /gene="tesB"
                     /locus_tag="ACHKIR_000885"
     CDS             complement(17802..18662)
                     /gene="tesB"
                     /locus_tag="ACHKIR_000885"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HFO9988668.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            18880..19449
                     /gene="ybaY"
                     /locus_tag="ACHKIR_000886"
     CDS             18880..19449
                     /gene="ybaY"
                     /locus_tag="ACHKIR_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HFO9988669.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQPAI
                     QQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFVL
                     PFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPVQ
                     ASGGATATVPSTSPTQVNPSSAVPAPTQY"
     gene            complement(19480..19791)
                     /locus_tag="ACHKIR_000887"
     CDS             complement(19480..19791)
                     /locus_tag="ACHKIR_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HFO9988670.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            19954..20050
                     /gene="ffs"
                     /locus_tag="ACHKIR_000888"
     ncRNA           19954..20050
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHKIR_000888"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            20170..20523
                     /gene="ybaA"
                     /locus_tag="ACHKIR_000889"
     CDS             20170..20523
                     /gene="ybaA"
                     /locus_tag="ACHKIR_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HFO9988671.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(20565..22115)
                     /gene="pdeB"
                     /locus_tag="ACHKIR_000890"
     CDS             complement(20565..22115)
                     /gene="pdeB"
                     /locus_tag="ACHKIR_000890"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HFO9988672.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELNTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPEPGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FPEMNISIITWSSTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDWLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(22279..22749)
                     /gene="ylaC"
                     /locus_tag="ACHKIR_000891"
     CDS             complement(22279..22749)
                     /gene="ylaC"
                     /locus_tag="ACHKIR_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HFO9988673.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(22865..23416)
                     /gene="maa"
                     /locus_tag="ACHKIR_000892"
     CDS             complement(22865..23416)
                     /gene="maa"
                     /locus_tag="ACHKIR_000892"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HFO9988674.1"
                     /translation="MSTEKEKMIAGELYHSADEALSRDRLRARRLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
     gene            complement(23589..23807)
                     /gene="hha"
                     /locus_tag="ACHKIR_000893"
     CDS             complement(23589..23807)
                     /gene="hha"
                     /locus_tag="ACHKIR_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HHA domain-containing protein"
                     /protein_id="HFO9988675.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(23833..24207)
                     /gene="tomB"
                     /locus_tag="ACHKIR_000894"
     CDS             complement(23833..24207)
                     /gene="tomB"
                     /locus_tag="ACHKIR_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HFO9988676.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(24753..27902)
                     /gene="acrB"
                     /locus_tag="ACHKIR_000895"
     CDS             complement(24753..27902)
                     /gene="acrB"
                     /locus_tag="ACHKIR_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease AcrB"
                     /protein_id="HFO9988677.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(27925..29118)
                     /gene="acrA"
                     /locus_tag="ACHKIR_000896"
     CDS             complement(27925..29118)
                     /gene="acrA"
                     /locus_tag="ACHKIR_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HFO9988678.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     AAYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATTLATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            29260..29907
                     /gene="acrR"
                     /locus_tag="ACHKIR_000897"
     CDS             29260..29907
                     /gene="acrR"
                     /locus_tag="ACHKIR_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HFO9988679.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            30035..33397
                     /gene="mscK"
                     /locus_tag="ACHKIR_000898"
     CDS             30035..33397
                     /gene="mscK"
                     /locus_tag="ACHKIR_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HFO9988680.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRVKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLRDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            33458..33556
                     /locus_tag="ACHKIR_000899"
                     /pseudo
     CDS             33458..33556
                     /locus_tag="ACHKIR_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(33609..33770)
                     /gene="rsmS"
                     /locus_tag="ACHKIR_000900"
     CDS             complement(33609..33770)
                     /gene="rsmS"
                     /locus_tag="ACHKIR_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HFO9988681.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDDEAEK"
     gene            complement(33784..34311)
                     /gene="priC"
                     /locus_tag="ACHKIR_000901"
     CDS             complement(33784..34311)
                     /gene="priC"
                     /locus_tag="ACHKIR_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HFO9988682.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVAQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREATAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARATDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            34381..34758
                     /gene="ybaN"
                     /locus_tag="ACHKIR_000902"
     CDS             34381..34758
                     /gene="ybaN"
                     /locus_tag="ACHKIR_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HFO9988683.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            34911..35462
                     /gene="apt"
                     /locus_tag="ACHKIR_000903"
     CDS             34911..35462
                     /gene="apt"
                     /locus_tag="ACHKIR_000903"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFO9988684.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            35591..37522
                     /gene="dnaX"
                     /locus_tag="ACHKIR_000904"
     CDS             35591..37522
                     /gene="dnaX"
                     /locus_tag="ACHKIR_000904"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFO9988685.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQ
                     PQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERL
                     ASVTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPE
                     LAAKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNSVCLHLRSSQRHLNNR
                     GAQQKLAEALSTLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNI
                     QTLRRFFDAELDEESIRPI"
     misc_feature    36855..36919
                     /gene="dnaX"
                     /locus_tag="ACHKIR_000904"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            37575..37904
                     /gene="ybaB"
                     /locus_tag="ACHKIR_000905"
     CDS             37575..37904
                     /gene="ybaB"
                     /locus_tag="ACHKIR_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFO9988686.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            37904..38509
                     /gene="recR"
                     /locus_tag="ACHKIR_000906"
     CDS             37904..38509
                     /gene="recR"
                     /locus_tag="ACHKIR_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFO9988687.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            38619..40493
                     /gene="htpG"
                     /locus_tag="ACHKIR_000907"
     CDS             38619..40493
                     /gene="htpG"
                     /locus_tag="ACHKIR_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HFO9988688.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTHTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            40674..41318
                     /gene="adk"
                     /locus_tag="ACHKIR_000908"
     CDS             40674..41318
                     /gene="adk"
                     /locus_tag="ACHKIR_000908"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFO9988689.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRAALEKILG"
     gene            41450..42412
                     /gene="hemH"
                     /locus_tag="ACHKIR_000909"
     CDS             41450..42412
                     /gene="hemH"
                     /locus_tag="ACHKIR_000909"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HFO9988690.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEGGSPLMVYSRQQQQALAQRLPETPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(42409..43368)
                     /gene="aes"
                     /locus_tag="ACHKIR_000910"
     CDS             complement(42409..43368)
                     /gene="aes"
                     /locus_tag="ACHKIR_000910"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HFO9988691.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPPWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYTLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FTGDSAGAMLALASALWLRDKQIDCGKVAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            43520..44824
                     /gene="gsk"
                     /locus_tag="ACHKIR_000911"
     CDS             43520..44824
                     /gene="gsk"
                     /locus_tag="ACHKIR_000911"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HFO9988692.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(44908..46584)
                     /gene="ybaL"
                     /locus_tag="ACHKIR_000912"
     CDS             complement(44908..46584)
                     /gene="ybaL"
                     /locus_tag="ACHKIR_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HFO9988693.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKAIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSLAAFFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALDLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVAYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(46822..48042)
                     /gene="fsr"
                     /locus_tag="ACHKIR_000913"
     CDS             complement(46822..48042)
                     /gene="fsr"
                     /locus_tag="ACHKIR_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HFO9988694.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            48260..49912
                     /gene="ushA"
                     /locus_tag="ACHKIR_000914"
     CDS             48260..49912
                     /gene="ushA"
                     /locus_tag="ACHKIR_000914"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFO9988695.1"
                     /translation="MKLLQRGVALALLTTFTLASEAALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQAPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLIMAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNNLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKAGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(49949..50428)
                     /gene="ybaK"
                     /locus_tag="ACHKIR_000915"
     CDS             complement(49949..50428)
                     /gene="ybaK"
                     /locus_tag="ACHKIR_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HFO9988696.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(50632..51426)
                     /gene="ybaP"
                     /locus_tag="ACHKIR_000916"
     CDS             complement(50632..51426)
                     /gene="ybaP"
                     /locus_tag="ACHKIR_000916"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HFO9988697.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDISLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEKRISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDNGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNEITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLCAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            51559..51861
                     /locus_tag="ACHKIR_000917"
     CDS             51559..51861
                     /locus_tag="ACHKIR_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO9988698.1"
                     /translation="MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIIN
                     AATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYRLIFKWVNGKAEELFLDPHNY"
     gene            51897..52238
                     /gene="ybaQ"
                     /locus_tag="ACHKIR_000918"
     CDS             51897..52238
                     /gene="ybaQ"
                     /locus_tag="ACHKIR_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFO9988699.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(52296..54800)
                     /gene="copA"
                     /locus_tag="ACHKIR_000919"
     CDS             complement(52296..54800)
                     /gene="copA"
                     /locus_tag="ACHKIR_000919"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HFO9988700.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSVTEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTNEGEK
                     NVPLADVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADFDEAQALRLAAALEQGSSHPLAR
                     AILDKASDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAVDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            55063..55995
                     /gene="glsA"
                     /locus_tag="ACHKIR_000920"
     CDS             55063..55995
                     /gene="glsA"
                     /locus_tag="ACHKIR_000920"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFO9988701.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNIYRAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENTEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            55998..57290
                     /gene="ybaT"
                     /locus_tag="ACHKIR_000921"
     CDS             55998..57290
                     /gene="ybaT"
                     /locus_tag="ACHKIR_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFO9988702.1"
                     /translation="MMNTEGNNDNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGALLLSLIVAMVMKRNKTV"
     gene            57321..57418
                     /locus_tag="ACHKIR_000922"
                     /pseudo
     CDS             57321..57418
                     /locus_tag="ACHKIR_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308571.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            57415..57822
                     /gene="cueR"
                     /locus_tag="ACHKIR_000923"
     CDS             57415..57822
                     /gene="cueR"
                     /locus_tag="ACHKIR_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HFO9988703.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIEQHIEELQSMR
                     NQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(57823..58553)
                     /locus_tag="ACHKIR_000924"
                     /pseudo
     CDS             complement(57823..58553)
                     /locus_tag="ACHKIR_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000898666.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(58625..58831)
                     /locus_tag="ACHKIR_000925"
     CDS             complement(58625..58831)
                     /locus_tag="ACHKIR_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9988704.1"
                     /translation="MKEQRKIPLTHIMIIGAFIFAFLQVVLLASLVHAVNVNNEIQEG
                     LFQSGRIMVESLQHILSVQTGING"
     gene            complement(58828..59250)
                     /locus_tag="ACHKIR_000926"
     CDS             complement(58828..59250)
                     /locus_tag="ACHKIR_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9988705.1"
                     /translation="MMNSSIKSFSLLAVILLAGCSSPASRIADCQAQGVSHDTCYLAE
                     QQRQTAILSASEAQAFKNAEAAQHAQAAKKAIYKGFGMTFRMSSKNFAYLNDSLCAID
                     EDNADATVYQSGLYNVIVYHHTGKVALMKEGQFVGYLK"
     gene            complement(59336..60352)
                     /locus_tag="ACHKIR_000927"
     CDS             complement(59336..60352)
                     /locus_tag="ACHKIR_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFO9988706.1"
                     /translation="MKTVNVALLALIISATSSPVVLAGDTIEAAATELSAINSGMSQS
                     EIEQKITRFLERTDNSPAAYTYLTEHHYIPSETPDTTQTPTVQTDPDAGQKTVAATGD
                     TQTPARYQSMINARQSAVTDAQQTQITEQQAQIVATQKTLAATGDTQNTAHYQEMINA
                     RLAAQNEANQRTTTEQGQKMNALTTDVAAQQQKERAQYDKQMQSLAQKSVQAHEQIES
                     LRQDSAQTQQQLSNTQKRVADNSQQINTLNNHYSSLKNEVEDNRKEANAGTASAIAIA
                     SQPQVKTGDVMMVSAGAGTFNGESAVSVGTSFNAGTHTVLKAGISADTQSDFGAGVGV
                     GYSF"
     gene            60719..61081
                     /locus_tag="ACHKIR_000928"
     CDS             60719..61081
                     /locus_tag="ACHKIR_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9988707.1"
                     /translation="MNEFEKIFNEMNLDRALLPILFRSNRSTVWKYLSGDSTAPASAM
                     SLIMLLQLIQKRNPDLLAEWLTLSDFTIPPEVYLDQPDYWKGWVYTQHKVNKNVLEYL
                     KKHYPDEDQKSMGKGREE"
     gene            complement(61184..61642)
                     /gene="ybbJ"
                     /locus_tag="ACHKIR_000929"
     CDS             complement(61184..61642)
                     /gene="ybbJ"
                     /locus_tag="ACHKIR_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFO9988708.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLLPLGWEWQGVMFAVLTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLSAGTHVEVIAIEGITLIIRAVIA"
     gene            complement(61639..62556)
                     /gene="qmcA"
                     /locus_tag="ACHKIR_000930"
     CDS             complement(61639..62556)
                     /gene="qmcA"
                     /locus_tag="ACHKIR_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFO9988709.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            62702..63379
                     /gene="fetA"
                     /locus_tag="ACHKIR_000931"
     CDS             62702..63379
                     /gene="fetA"
                     /locus_tag="ACHKIR_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HFO9988710.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNQQPDPAIFLDFLERFALPDTILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            63366..64145
                     /gene="fetB"
                     /locus_tag="ACHKIR_000932"
     CDS             63366..64145
                     /gene="fetB"
                     /locus_tag="ACHKIR_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HFO9988711.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(64208..65062)
                     /gene="cnoX"
                     /locus_tag="ACHKIR_000933"
     CDS             complement(64208..65062)
                     /gene="cnoX"
                     /locus_tag="ACHKIR_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HFO9988712.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(65123..65932)
                     /gene="ybbO"
                     /locus_tag="ACHKIR_000934"
     CDS             complement(65123..65932)
                     /gene="ybbO"
                     /locus_tag="ACHKIR_000934"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFO9988713.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNSMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHCGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(65922..66545)
                     /gene="tesA"
                     /locus_tag="ACHKIR_000935"
     CDS             complement(65922..66545)
                     /gene="tesA"
                     /locus_tag="ACHKIR_000935"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016298 - lipase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HFO9988714.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            66516..67202
                     /gene="ybbA"
                     /locus_tag="ACHKIR_000936"
     CDS             66516..67202
                     /gene="ybbA"
                     /locus_tag="ACHKIR_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter ATP-binding protein
                     YbbA"
                     /protein_id="HFO9988715.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            67199..69613
                     /gene="ybbP"
                     /locus_tag="ACHKIR_000937"
     CDS             67199..69613
                     /gene="ybbP"
                     /locus_tag="ACHKIR_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease subunit YbbP"
                     /protein_id="HFO9988716.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGSENQLDGYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSIERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLALSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMLLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVAPLKAF
                     LAEHQIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFAG"
BASE COUNT        16975 a        17606 c        18557 g        16673 t
ORIGIN      
        1 catgaatttt gcatggaacc gtgcgaaaag cctctttcgg tgttagcgta acaacaaaag
       61 attgttatgc ttgaaatatg gtgatgccgt acccataaca cagggactag ctgataatcc
      121 gtccataagg ttacaatcgg tacagcaggt tttttcaatt ttatccagga gacggaaatg
      181 tcatacagcg gcgaacgaga taactttgca ccccatatgg cgctggtgcc gatggtcatt
      241 gaacagacct cacgtggtga gcgctctttt gatatctatt ctcgtctact taaggaacgc
      301 gtcatttttc tgactggcca ggttgaagac cacatggcta acctgattgt ggcgcagatg
      361 ctgttcctgg aagcagaaaa cccagaaaaa gatatctatc tgtacattaa ctccccaggc
      421 ggggtgatca ctgccgggat gtctatctat gacaccatgc agtttatcaa gcctgatgtc
      481 agcaccatct gtatgggcca ggcggcttcg atgggcgctt tcttgctgac tgcaggggca
      541 aaaggtaaac gtttctgcct gccgaattcg cgcgtgatga ttcaccagcc gttgggcggc
      601 taccagggcc aggcgaccga tatcgaaatt catgcccgtg aaattctgaa agttaaaggg
      661 cgcatgaatg aacttatggc gcttcatacg ggtcaatcat tagaacagat tgaacgtgat
      721 accgagcgcg atcgcttcct ttccgcccct gaagcggtgg aatacggtct ggtcgattcg
      781 attctgaccc atcgtaattg atgccagagg cgcaactgtg ccgctatact tatccagggc
      841 ggcacaacgc tgtaagcggc ttgcgcctga gaatggcatt tgcgtcgtcg tgtgcggcac
      901 aaagaacaaa gaagaggttt tgacccatga cagataaacg caaagatggc tcaggcaaat
      961 tgctgtattg ctctttttgc ggcaaaagcc agcatgaagt gcgcaagctg attgccggtc
     1021 catccgtgta tatctgcgac gaatgtgttg atttatgtaa cgacatcatt cgcgaagaga
     1081 ttaaagaagt tgcaccgcat cgtgaacgca gtgcgctacc gacgccgcat gaaattcgca
     1141 accacctgga cgattacgtt atcggccagg aacaggcgaa aaaagtgctg gcggtcgcgg
     1201 tatacaacca ctacaaacgt ctgcgcaacg gcgataccag caatggtgtc gagttgggca
     1261 aaagtaacat tctgctgatc ggtccgaccg gttccggtaa aacgctgctg gctgaaacgc
     1321 tggcgcgcct gctggacgtc ccgttcacca tggccgacgc aaccacgctg accgaagccg
     1381 gttatgtggg cgaagacgtt gaaaacatca ttcagaagct gttgcagaag tgcgattacg
     1441 acgtacagaa agcgcagcgc gggattgtct acatcgatga aatcgacaag atttctcgta
     1501 agtcagacaa cccgtccatt acccgagacg tttccggtga aggcgtacag caggcactgt
     1561 tgaaactgat cgaaggtacg gtagctgctg ttccaccgca aggtgggcgt aaacatccgc
     1621 agcaggaatt cttgcaggtt gatacctcta agatcctgtt tatttgtggc ggtgcgtttg
     1681 ccggtctgga taaagtgatt tcccatcgtg tagaaaccgg ctccggcatt ggttttggcg
     1741 cgacggtaaa agcgaagtcc gacaaagcaa gcgaaggcga gctgctggcg caggttgaac
     1801 cggaagatct gatcaagttt ggtcttatcc ctgagtttat tggtcgtctg ccggttgtcg
     1861 caacgttgaa tgaactgagc gaagaagctc tgattcagat cctcaaagag ccgaaaaacg
     1921 ccctgaccaa gcagtatcag gcgctgttta atctggaagg cgtggatctg gaattccgtg
     1981 acgaggcgct ggatgctatc gctaagaaag cgatggcgcg taaaaccggt gcccgtggcc
     2041 tgcgttccat cgtagaagcc gcactgctcg ataccatgta cgatctgccg tccatggaag
     2101 acgtcgaaaa agtggttatc gacgaatcgg taattgatgg tcaaagcaag ccgttgctga
     2161 tttatggcaa gccggaagcg caacaggcat ctggtgaata attaaccatt cccatacaat
     2221 tagttaacca aaaagggggg attttatctc ccctttaatt tttcctctat tctcggcgtt
     2281 gaatgtgggg gaaacatccc catatactga cgtacatgtt aatagatggc gtgaagcaca
     2341 gtcgtgtcat ctgattacct ggcggaaatt aaactaagag agagctctat gaatcctgag
     2401 cgttctgaac gcattgaaat ccccgtattg ccgctgcgcg atgtggtggt ttatccgcac
     2461 atggtcatcc ccttatttgt cgggcgggaa aaatctatcc gttgtctgga agcggcgatg
     2521 gaccatgata aaaaaattat gctggtcgcg cagaaagaag cttcaacgga tgagccgggt
     2581 gtaaacgatc ttttcaccgt cgggaccgtg gcctctatat tgcagatgct gaaactgcct
     2641 gacggcaccg tcaaagtgct ggtcgagggg ttacagcgcg cgcgtatttc tgcgctctct
     2701 gacaatggcg aacacttttc tgcgaaggcg gagtatctgg agtcgccgac cattgatgag
     2761 cgggaacagg aagtgctggt gcgtactgca atcagccagt tcgaaggcta catcaagctg
     2821 aacaaaaaaa tcccaccaga agtgctgacg tcgctgaata gcatcgacga cccggcgcgt
     2881 ctggcggata ccattgctgc acatatgccg ctgaaactgg ctgacaaaca gtccgttctg
     2941 gagatgtccg acgttaacga acgtctggaa tatctgatgg caatgatgga atcggaaatc
     3001 gatctgctgc aggttgagaa acgcattcgc aaccgcgtta aaaagcagat ggagaaatcc
     3061 cagcgtgagt actatctgaa cgagcaaatg aaagctattc agaaagaact cggtgaaatg
     3121 gacgacgcgc cggacgaaaa cgaagccctg aagcgcaaaa ttgacgcggc gaaaatgccg
     3181 aaagaggcaa aagagaaagc ggaagcagag ttgcagaagc tgaaaatgat gtctccgatg
     3241 tcggcagaag cgaccgtagt gcgtggttat atcgactgga tggtacaggt gccgtggaat
     3301 gcgcgcagca aggtcaaaaa agacctgcgt caggcgcagg aaatccttga taccgaccat
     3361 tatggtctgg agcgcgtgaa agatcgcatc cttgagtatc ttgcggttca aagccgtgtc
     3421 aacaaaatca agggaccgat cctctgcctg gtagggccgc cgggggtagg taaaacctct
     3481 cttggtcagt ccattgccaa agccaccggg cgtaaatatg tccgtatggc gctgggcggc
     3541 gtgcgtgatg aagcggaaat ccgtggtcac cgccgtactt acatcggttc tatgccgggt
     3601 aaactgatcc agaaaatggc gaaagtgggc gtgaaaaacc cgctgttcct gctcgatgag
     3661 atcgacaaaa tgtcttctga catgcgtggc gatccggcct ccgcactgct tgaagtgctg
     3721 gatccagagc agaacgtagc gttcagcgac cactacctgg aagtggatta cgaccttagc
     3781 gacgtgatgt ttgtcgcgac gtcgaactcc atgaacattc cggcaccgct gctggatcgt
     3841 atggaagtga ttcgcctctc cggttatacc gaagatgaaa aactgaacat cgccaaacgt
     3901 cacctgctgc cgaagcagat tgaacgtaat gcactgaaaa aaggtgagct gaccgtcgac
     3961 gatagcgcca ttatcggcat tattcgttac tacacccgtg aggcgggcgt gcgtggtctg
     4021 gagcgtgaaa tctccaaact gtgtcgcaaa gcggttaagc agttactgct cgataagtca
     4081 ttaaaacata tcgaaattaa cggcgataac ctgcatgact atctcggtgt tcagcgcttc
     4141 gactatggtc gcgcggataa cgaaaaccgt gtcggtcagg taactggtct ggcgtggacg
     4201 gaagtgggcg gtgacttgct gaccattgaa accgcgtgcg ttccgggtaa aggcaaactg
     4261 acctataccg gatcgcttgg cgaagtgatg caggagtcca ttcaggcggc gttaacggtg
     4321 gttcgtgcgc gtgcggaaaa actggggatc aaccctgatt tttatgaaaa acgcgacatc
     4381 cacgtccacg taccggaagg tgcgacgccg aaagatggtc cgagtgccgg tattgctatg
     4441 tgcaccgcgc tggtttcttg cctgaccggt aacccggttc gtgctgatgt ggcaatgacc
     4501 ggtgagatca ctctgcgtgg tcaggtactg cctatcggtg gtttgaaaga aaaactactg
     4561 gcagcgcatc gcggcgggat caaaacagtg ttaattccgt tcgaaaataa acgcgatctg
     4621 gaagagattc ctgacaacgt aattgccgat ctggatattc atcctgtgaa acgcattgag
     4681 gaagttctga ctctggcgct gcaaaatgaa ccgtctggca tgcaggttgt gactgcaaaa
     4741 tagtgacctc gcgcaaaatg cactaataaa aacagggctg gcaggctaat tcggacttgc
     4801 cagccttttt ttgtctcgct aagttagatg gcggattggg cttgccctta ttaaggggtg
     4861 ttgtaagggg atggctggcc tgatataact gctgcgcgtt cgtaccttga aggattcaag
     4921 tgcgatataa attataaaga ggaagagaag agtgaataaa tctcaattga tcgacaagat
     4981 tgctgcaggg gctgatatct ctaaagctgc ggctggccgt gcgttagatg ctattattgc
     5041 ttccgtaact gaatctctga aagaagggga tgatgtagca ctggtaggtt ttggtacttt
     5101 tgccgttaaa gagcgtgctg cccgtactgg ccgcaatccg cagaccggta aagagatcac
     5161 catcgctgct gctaaagtac cgagcttccg tgcaggtaaa gcactgaaag acgcggtaaa
     5221 ctaagcgttg tccccagtgg ggatgtgacg aagttcaagg gcgcatctac tgatgtgcct
     5281 tttttatttg tattcggtga ctttctgcgt cttgtgggct gacaattgcc cccgtttctt
     5341 gtcacaatat gcctttgcgc gcatcgatac gttgcgtgag gtacacagtc atctacagcg
     5401 gagtgttgtt acaccatgat ggacagctta cgcacggctg caaacagtct cgtgctcaag
     5461 attattttcg gtatcattat cgtgtcgttc atattgaccg gcgtgagtgg ttacctgatt
     5521 ggcggaggca ataactacgc cgcaaaagtg aatgaccagg aaatcagtcg tgggcaattc
     5581 gagaacgcct tcaacagcga gcgtaatcgc atgcagcaac agctgggcga tcaatactcc
     5641 gagctggcag cgaacgaagg ctatatgaaa accctgcgtc aacaggtgct gaatcgtctg
     5701 atcgacgagg cgctgctgga tcagtacgct cgtgagctga aactgggtat cagcgatgag
     5761 caggttaaac aggcgatttt cgcgacccca gccttccagg ttgacggcaa atttgataac
     5821 agccgctata acggtatcct caaccagatg gggatgaccg ccgatcagta cgcccaggcg
     5881 ctgcgtaacc agctcactac ccaacagctg attaacggcg ttgccggtac cgattttatg
     5941 ctgaaaggtg aaaccgacga gctggcggca ctggtagttc aacaacgcgt ggtgcgtgaa
     6001 gcgactatcg atgttaacgc gctggcggcg aagcagcctg tgaccgaaca ggaaattgcc
     6061 agctactacg aacaaaacaa aaacaatttc atgacgccgg aacaattccg cgtgagttac
     6121 atcaagctgg atgccgcaac gatgcagcaa ccggttagcg atgcggatat ccagagctac
     6181 tacgatcagc atcaggatca attcacccag ccgcagcgta cccgttacag catcatccag
     6241 accaaaactg aagatgaagc gaaagcggta cttgatgagc tgaataaggg cggtgatttt
     6301 gctgcattag ccaaagaaaa atctgccgat attatctctg cccgcaacgg cggtgatatg
     6361 ggttggttag aagatgccac tatcccggac gaactgaaaa atgctggtct gaaaaaaaaa
     6421 ggccaactgt ctggtgttat caaatcttcg gtcggtttcc tgattgtgcg tctggacgac
     6481 attcagccag cgaaagtgaa atcgttagac gaagtacgtg acgacattgc ggcgaaagtg
     6541 aaacacgaaa aagccctcga tgcgtactac gcgctgcagc agaaagtgag cgatgcggca
     6601 agcaacgaca ccgagtctct ggccggtgca gagcaagctg ccggcgttaa agccactcag
     6661 acgggttggt tcagcaaaga taacctgccg gaagagttga acttcaagcc agttgccgac
     6721 gctatcttca acggcggtct ggtcggtgaa aacggcgcgc cgggcatcaa ctctgacatc
     6781 atcaccgtag acggcgaccg cgcattcgtg ctgcgcatca gcgagcacaa accggaagcg
     6841 gtgaaaccgt tggcagatgt tcaggaacaa gttaaggcac tggttcagca caacaaagct
     6901 gaacaacagg cgaaagtgga tgctgagaaa ctgctggttg atttgaaagc aggcaaaggt
     6961 gcggaagcta tgcaggctgc cggtctgaaa tttggcgagc cgaaaacctt aagtcgttcc
     7021 ggtcgtgacc cgattagcca ggctgcgttt gcactgccgc tgccagcgaa agacaaaccg
     7081 agctacggta tggcgaccga tatgcaaggt aatgtagttc tgctggcgct ggatgaagtg
     7141 aagcaaggtt caatgccgga agatcagaaa aaagcgatgg tgcagggtat cacccagaac
     7201 aacgcacaaa tcgtctttga agctctgatg agtaacctgc gtaaagaggc gaaaatcaaa
     7261 attggcgatg cgctggaaca gcaataatcc tgaagccgcc tcgcaaaaaa atgctttgca
     7321 gctgtaacaa gaaaaggtcg ctttcgcggc cttttccatt tctgaacatt gccatttgtt
     7381 tactgttttc actgccgtta aggtgattcc actgttaaca aacaaggaga aaacagtatg
     7441 aaacacggaa ttaaagcact gctcattacc ctgtccctgg cctgtgccgg aatgtctcat
     7501 agtgctctgg cggcagctcc tgcggcaaaa ccgacaacgg tagaaaccaa agcggaagct
     7561 cctgcagcac aaagtaaagc agcagttccg gcgaaagcca gtgacgaaga aggcacccgg
     7621 gtcagcatta ataatgccag cgcggaagag ttagcccgcg cgatgaatgg cgttggcctg
     7681 aagaaagcgc aggcgattgt cagttatcgc gaagagtacg gtccgtttaa aacggtggag
     7741 gatctaaagc aggtgccggg gatgggcaat tcgctggtgg agcgtaatct ggcggtatta
     7801 accctgtaat taatttgcat agtggcaatt ttttgccaga ctgaagaggt cataccagtt
     7861 atgacctctg tacttataac aacaacgtaa ggttctcgcg ctatgcaaac acaaatcaaa
     7921 gttcgtggat atcatctcga tgtttaccag cacgttaata acgcccgcta tctggagttt
     7981 ctcgaagaag cccgctggga tgggttggaa aatagcgaca gttttcagtg gatgacggcc
     8041 cataacatcg ctttcgtggt ggtgaatatc aatattaact atcgtcgccc ggcggtatta
     8101 agcgacttgt taaccattac gagccagtta cagcaattaa acggtaaaag cggcatctta
     8161 agccaggtca ttacactgga gccggaaggg caggtggtag cggatgcgct tattacgttt
     8221 gtttgtattg atcttaaaac gcagaaagca ttagctctgg aaggggaatt gcgcgaaaag
     8281 ctcgagcaga tggttaagta aacgttttgt ggtgccggat gctcaagccg catccggcga
     8341 cacccggaat aattacttca acccggtttt ctgcttcatc gctgccatca ccgtcggttt
     8401 atcggccaga taatgatgca aaccgttggc acgtaaatta cacgccgcac aatgaccgca
     8461 accgtcgcct ttaatgccgt tataacaggt cagcgtttcg ttacggacta aatccagctt
     8521 gccgtaataa tctgccagcg cccaggtttc cgctttatca atccacatca gcggcgtttc
     8581 aaaacgaatg tctttcgcca tacccagact gacggcatgg ttcagtgctt tcacaaactc
     8641 atcgcggcaa tccgggtagc cggagaaatc cgtttcgcag acgccggtaa tcaccgcttc
     8701 tgcttttacc tgatacgcat atattgccgc cagtgtcagg aacaaaatat tacgcccagg
     8761 gacaaacgta ttcgggatgc catcggcttc aggttcataa tctggcactg gaatgctgtc
     8821 acgtgtcagg ctgctgaccg ccagctcgtt gagcagcgtt acatcaagaa ccttatgcgc
     8881 gcgtgccccc agtttcagcg ccagttcgcg tgccacgtcg atttccgcgc gatgtcgctg
     8941 accgtaatcg aacgtcacgc aatggacttc atcatattgt tgtaatgcct gcaccagaca
     9001 ggtggtagaa tcctggcctc cactgaacac aacgacagca cgtttcatag ataatcctgc
     9061 ttgaacaata aaagcgttat ggtaacgcct gcgatgaacc cggaccagct attcattgcg
     9121 atggcgcggg aagccaggct tcggtaaatt caaaccagcc tcgcggtgtc agtcgcacac
     9181 cgttcacgcc gggaggggcg ctaatgcgat agtgatagtt gaacagcggc gtcagcgtcg
     9241 catctgccat taactggcta aaaaccgctt tcagggcatt aaatcggttt tcctcatcag
     9301 gcattacttg caccgcgtcc agtgtcgatt gcaaatgtgc atatgctgga gcgtcgaaaa
     9361 catgtggcca tagtggatcg caacgcagcc attgctccag ggtatattcc ggtgcttcgc
     9421 caattaatct gtcgcccatc atgaggtctg cgtgtgccag tagggtcgtg tcgtcccagt
     9481 tttttgcgtt atgaaaaata attgtgagtt cacagccttc cgctgccagt gtcgcctgta
     9541 gacgttctgc catggtatga agttctatcg gtaggtgata aaccagcgtc aaggttttcg
     9601 gtagtttgac ctcatccggt acttgccatt gcggaatagt ccagcctggc agtaatgcat
     9661 gactggcggt gatcaggttt tctccgactt ctaacgtttg taataaaccg gattgatgaa
     9721 taatggaaat cacttttcgc gcctgccaga gggacagtcg gggacttttg cgcaacgtta
     9781 aatagcaaaa acctaaactg atgccactac tgacctggct gacccgttgc agctcctccg
     9841 gtttgccgat ggtgatttgc acgggatgcc gacaactggt tcccaaatct ttttcgaaaa
     9901 gcggcggagt tatccagtac tcaaccgctt taagcagcgg atgacgtaaa tggtaataat
     9961 catggctttc caggcgcacc agctccgctg tgaattgtgt taagcgaaaa ggacccgtgc
    10021 cgatcagtgg gaattgcgga tgcgccagat ggctgcaata gctcgccagt cggtgcgcaa
    10081 gccagtaatc ggggcgatgt aaaaagaagg tcagacactg cggatgggtg acttcaatac
    10141 gcttcacgct aataaataat tgatcccgtg ctggcagttg taacagcagc aataatcgct
    10201 ggtgtaagtg tgaggctttt actgcatcgc cgttatgcca gtgtagggtt gaacgaagat
    10261 aaaagtccca gcgtaacccg tcagtagagg tttcccagtg atgtgctaaa tcgccaatcg
    10321 ggcgctgagt attattatcg aagcgggtca ggccggaaaa tatctgcccg gccagatgct
    10381 gctcggcacg gccgggcaaa aagcccggtt gtagcggttc gagcgggcga tagtagggaa
    10441 tacgcaacgt gggcgtatcg ttttgccatt gtccgcccat aaacggctgt aacagagtgc
    10501 gcagctcacc tggggccagt tgcgccagct ccagcacatc ttgctgcttt ccggtttcca
    10561 gcgcctgttc catcatcgca ttgcgtagcg attctggcgt gaccagaaag cgtagttgcc
    10621 cgcgttttcc gcgtcctgac tgcgcctgcc actccagcca tcccgcctcc tgtgcctggc
    10681 gtaacagcgt acgaacatgg cgttcgctgc aaaaacagcg ttcggccagt tcgctgacgg
    10741 tgacggtttg cggctttccg gcggaaggtt gccacagacg ttgatactgg ttaagacggt
    10801 tgagcagtcg catataaacc cggaacaata ttatttaact attcactatt acttccgtat
    10861 atatcaggtg atactcaatc accattaacc gtgtcacaga gtggagaaag aaatggctcg
    10921 tctggcagca tttgatatgg atggcacttt attgatgcct gaccatcatt taggtgagaa
    10981 aaccctctct actttggcgc gactgcgtga acgcgacatt accctcactt ttgccactgg
    11041 gcgtcatgcg ctggagatgc agcatattct cggggcgcta tcgctggatg catatttgat
    11101 taccggcaac ggaacgcgcg tgcattctct ggaaggtgaa cttttacatc gtgatgattt
    11161 acctgcggat gtcgcggagc tggtgctgta tcagcagtgg gatacccgag ccagcatgca
    11221 tatcttcaat gacgacggtt ggtttaccgg gaaagagatc cctgcgttgt tgcaggcatt
    11281 tgtctatagt ggttttcgtt atcagataat cgatgtcaaa aaaatgccac tcggcagcgt
    11341 caccaagatc tgcttctgtg gcgatcacga cgatcttaca cgcttgcaga tccagctata
    11401 cgaagcatta ggcgagcgtg cacatttgtg tttttccgcc acggattgcc tcgaagtgct
    11461 gccggtgggc tgcaataaag gcgctgcatt gacggtgctg acccaacatt taggtttatc
    11521 gttgcgcgat tgcatggcct ttggtgatgc gatgaacgat cgcgaaatgt taggcaacgt
    11581 cggtagcgga tttattatgg gcaatgcgat gccgcaattg cgcgcggagc tcccgcattt
    11641 accggtgatt ggacattgcc gaaatcaggc tgtctctcac tatttgacgc actggctgga
    11701 ctatccacat ctaccttatt cccccgaata acgagatccc ttccagcacc gggcaattgc
    11761 ccggtttttt ttgcgttgaa tttgtcattt tgtgccgtgg tgtttaaacc gcacagaata
    11821 aattgtcgcg atttcacctt taaaatagaa ttaaaagaga aaaaaattct ctgtggaagg
    11881 gctatgttag ataaaattga ccgtaagctg ctggccttac tacagcagga ttgtaccctc
    11941 tctttgcagg cactggctga agccgttaat ctgacaacca ccccgtgctg gaagcgtctg
    12001 aaacggctgg aggacgacgg tattcttatc ggcaaagtcg ccctgctgga tccggaaaaa
    12061 ataggcctcg gcctgaccgc ctttgtgctg ataaaaacgc aacatcacag cagcgaatgg
    12121 tattgccgct ttgtcacggt ggttaccgaa atgccagaag tgctggggtt ctggcgcatg
    12181 gccggtgaat acgattatct gatgcgcgtc caggttgccg acatgaaacg ctacgacgag
    12241 ttttataagc gtttggtaaa cagcgtgccg gggctgtcgg acgtcacttc cagcttcgcg
    12301 atggaacaga ttaaatacac cacttcttta cccatcgaat aaatatccag aatcaggtca
    12361 ggacacaacg cgtgcgatta tttgctcaat taagctggta tttccgtcgg gaatggcgtc
    12421 gctatctcgg ggctgtcgcc ttgcttgtca ttatcgcgat gctgcaactg gttccgccaa
    12481 aagtggttgg tattgttgtt gatggcgtga cagaacaaca ctttactacc gggcagatcc
    12541 tgatgtggat cgccaccatg gtgctgattg ccgttgtggt ttatctcctg cgttacgtct
    12601 ggcgtgtatt gctgtttggc gcgtcttatc aactggctgt tgaactgcgt gaagattatt
    12661 accgtcagct aagccggcag catcctgagt tttacctgcg tcatcgcacg ggtgacctca
    12721 tggctcgtgc gaccaatgac gtcgatcgcg tcgtgtttgc cgccggagaa ggggtgctga
    12781 cgctggtgga ttcactggta atgggctgcg ctgtgttgat tatgatgtct acgcaaatta
    12841 gctggcagtt gaccttattt gccctgttgc cgatgccagt gatggcgatc atgattaagc
    12901 gcaacggcga tgccttgcat gaacgcttta agctggcgca ggcggcgttt tccagtctta
    12961 atgaccgcac ccaggaaagc ctcaccagta tccgcatgat caaagctttt ggtctggaag
    13021 atcgccagtc ggcgttattt gccgaggatg ccgaagatac cggcaaaaaa aacatgcggg
    13081 tggcgcgtat tgatgctcgt ttcgacccga ccatctatat cgcgattggt atggcgaact
    13141 tgctggcgat tggcggcggt agttggatgg tggtgcaggg cagtttaacg ctgggccagc
    13201 tcaccagttt tatgatgtat ttaggtctga tgatttggcc aatgctggcg ctggcatgga
    13261 tgtttaacat agtggaacgt ggtagtgctg cgtacagccg cattcgcgcg atgctggcgg
    13321 aagcgccggt ggtgaatgat ggtagcgaac ccgtgccgga aggtcgtggc gaactggatg
    13381 taaatattcg ccagttcacg tatccgcaga ctgaccatcc tgcgctggaa aacgtcaatt
    13441 tcgccctgaa acccggtcag atgctgggta tttgcgggcc gactggttcc ggcaaaagta
    13501 ccctgttgtc gctcattcag cgtcatttcg acgtcagcga gggggatatc cgctttcatg
    13561 atattcctct gacgaagtta caactcgata gctggcgtag ccgcctggcg gtagttagcc
    13621 agacaccgtt ccttttttcc gacaccgtag cgaacaacat cgcgttgggt tgcccgaatg
    13681 ccacccagca agagattgag catgtcgcgc ggttagccag cgtgcatgac gatattttgc
    13741 gtttaccgca aggttacgat acagaagtgg gcgagcgcgg cgtgatgctt tccggtgggc
    13801 aaaaacagcg tatctccatt gctcgtgcgt tattagtcaa cgcggaaatc ctcatccttg
    13861 atgatgcgct ttcggcagta gacggacgca cagagcacca gatcctgcat aacctgcgtc
    13921 agtgggggca gggaagaacg gtaatcatca gcgcccatcg cctttctgca ctgacggaag
    13981 ccagtgaaat tattgtgatg cagcacggac atatcgctca gcgtggcaat catgatgagc
    14041 tggcgcaaca aagcggctgg tatcgcgata tgtatcgcta tcaacaactg gaggcggcgc
    14101 tcgacgacgc tccggaaatt cgcgaggagg ccatcgatgc gtagttttag ccaactgtgg
    14161 ccgactctca agcgtctgtt agcgtacggt tcgccgtggc gtaaaccgct ggggattgcg
    14221 gtcataatga tgtgggttgc ggcagcggca gaagtcagtg ggccgctgct tatcagctat
    14281 tttatcgaca atatggtagc gaaaaataac ctgccgttga aagtggttgc agggctggct
    14341 gcggcgtatg ttgggctgca actgtttgcc gccgggctac attacgcgca gtcgctgctg
    14401 tttaatcggg cggcagtagg cgtagtgcaa cagttgcgta ccgacgtgat ggatgctgcg
    14461 ttacgccagc cattaagcga gtttgatacc caacccgtcg ggcaggtgat ttcccgcgtc
    14521 actaatgaca ctgaagtgat ccgcgacctc tacgttaccg tagtggcaac tgtcctgcgt
    14581 agcgccgcac tggtgggcgc gatgctggtg gcgatgttca gcctcgactg gcgaatggcg
    14641 ctggtggcga taatgatttt cccggtggtg ctggtggtaa tggtgatata tcagcgttac
    14701 agcacgccga ttgtccgtcg tgtgcgcgct tatctggcag atatcaacga cggctttaac
    14761 gaaattatca atggcatgag cgttatccag cagttccgtc agcaggcgcg atttggcgaa
    14821 cgtatgggtg aggccagtcg ttcacactat atggcgagga tgcaaaccct gcgcctcgac
    14881 ggttttctgc tgcgtccgct gctgagtctg ttttcatcgc tcattctttg tggcttgttg
    14941 atgctgtttg gcttctccgc cagcggcacc attgaagtgg gcgtgttgta tgcgttcatc
    15001 agttatttgg ggcgacttaa cgaaccctta atcgaactga ccacgcagca ggcgatgctg
    15061 caacaggctg ttgttgctgg tgagcgcgtt tttgagctga tggacggacc gcgccagcaa
    15121 tatggcaatg atgatcgccc gttgcagagc ggcaccatcg acgtcgataa cgtgtcattt
    15181 gcttatcgcg atgacaatct ggtgctaaag aacattaatc tctctgtgcc ttcgcgcaac
    15241 tttgtggcgc tggtcgggca taccggcagt ggcaaaagca ccctcgccag tttattgatg
    15301 ggctattacc cgctaacgga aggtgagatt cgccttgatg gtcgtccatt aagtacgcta
    15361 agtcacagcg cgctgcgcca gggcgtggca atggtgcagc aagatccggt ggtgctggcg
    15421 gataccttcc tcgccaacgt gacgctggga cgtgatatct ctgaagaacg cgtctggcag
    15481 gcgctggaaa tcgtgcagct ggcggagctg gcgcgtagca tgagtgatgg tatttacacg
    15541 ccgcttggcg agcaggggaa taatctctca gtcgggcaaa agcaactgct ggcactggcg
    15601 cgcgtgctgg tggagacgcc gcaaatcctg atccttgatg aggcaaccgc cagcattgac
    15661 tccgggactg aacaggcgat tcaacatgct ctggcggcgg tgcgtgaaca tactacgctt
    15721 gtggtgattg ctcaccgctt atcgactatt gttgatgcca acaccattct ggtgcttcat
    15781 cgtgggcaag ccgtggagca gggcactcac cagcaactgc tggcggccca gggacgatac
    15841 tggcagatgt atcaactgca acttgcgggc gaagagctgg cagccagcgt gcgtgaagag
    15901 gaatcattga gcgcctgaat agcgcaatat ttcatcgttg gtgcaaaaat gtaacgcact
    15961 gtgcactgtc atagtgcgtt ttcattttca atcttcttaa cttcctgctc tctttctcgt
    16021 ttttcatttt tggcacaccg cttgcaatac cttcttcgtg tagcagaacc attaccgaat
    16081 tctgaccgga ggggatctat gaagctggtg accgtgatta tcaaaccatt caaacttgaa
    16141 gacgttcgtg aagcgttatc ttccattggt attcagggcc tgaccgtcac cgaagtgaaa
    16201 ggtttcgggc gtcagaaagg acatgccgag ctgtaccggg gggcggaata cagcgtcaat
    16261 ttcctgccaa aagtaaaaat tgatgtggcg attgctgatg accaactcga tgaagtgatc
    16321 gatatcgtca gtaaggcggc ttacaccgga aaaattggcg acggcaaaat cttcgtcgct
    16381 gaattgcaac gcgtcattcg tattcgtacc ggcgaagccg acgaagcggc gctgtaatct
    16441 ctggcacaca gcaacaggaa cgaaaaatga agatagcgac gataaaaact gggcttgctt
    16501 cactggcgat gctgccggga ctggtaatgg ctgcacctgc ggtggccgat aaagccgaca
    16561 atgcgtttat gatgatttgt actgcgctgg tgctgtttat gactattccg gggattgccc
    16621 tgttttacgg tgggttgatt cgcggcaaaa acgtgctgtc gatgctgacg caggtgacgg
    16681 tgacgtttgc actggtctgt attctctggg tggtttacgg ttactcgctg gcgtttggtg
    16741 agggaaacaa cttcttcggc aacattaacg ggttgatgct gaaaaacatc gaactgacgg
    16801 cggtgatggg cagcatttat cagtatatcc acgtagcgtt tcagggatcg tttgcctgca
    16861 ttaccgtagg cttgatagtt ggagcactgg cggaacgaat ccgcttctca gctgtgttga
    16921 ttttcgtggt ggtatggctg acgctctcct acattccgat tgcgcatatg gtctggggcg
    16981 gcggtttgct ggcttctcac ggtgcactgg atttcgcggg tggcacggtg gtgcacatta
    17041 acgccgcaat cgccggtctg gtgggcgcgt atctgatagg caaacgcgtg ggcttcggca
    17101 aagaggcgtt taaaccgcac aacctgccga tggtcttcac cgggactgcc attctctata
    17161 tcggttggtt tggctttaac gccgggtcag cgggcacggc gaatgaaatc gcagcactgg
    17221 catttgtgaa tactgtggtc gcaacggcgg cggcaattct tggctggatc ttcggtgaat
    17281 gggcgctgcg tggtaagcct tcactgctgg gagcgtgttc aggcgcgatt gccggtctgg
    17341 tcggcgtgac gccagcgtgt ggctacattg gtgttggcgg cgcgttgatt atcggcgtgg
    17401 tagctggtct ggcgggcttg tggggcgtta ccatgctcaa acgcttgttg cgggtggatg
    17461 atccctgcga tgtcttcggt gtgcacggcg tttgtggcat tgtcggctgt atcatgaccg
    17521 ggatttttgc tgccagctcg ctgggcggcg tgggcttcgc tgaaggtgtg acgatgggcc
    17581 atcagttgct ggtacagctg gaaagcatcg ccattacgat cgtctggtcc ggtgtggtgg
    17641 catttattgg ctacaaactg gcggatctga cggttggtct gcgtgtgccg gaagaacaag
    17701 agcgagaagg gctggacgtc aacagccacg gcgagaatgc ctataacgcg taacaagcac
    17761 tgcaaaaaac agccggacgg ttttcacctc cggctatttt tttaattatg attacgcatt
    17821 accccttcct gaacggtcga ggcaaccagc acgccgtcct gagtataaaa ctcaccgcgc
    17881 acaaagccgc gtgcgctgga cgccgaggtg ctctccacgc tatacagcag ccattcattc
    17941 aaattaaacg ggcgatggaa ccacatggaa tggtcgatgg tcgctatctg aattcccggt
    18001 tcgagaaaac cgatgccgtg cggctgtaga gctaccggca ggaagttaag atcagaagcg
    18061 taaccgagca gatactgatg aacgcgcaga tcatccggca cgctaccatt tgcgcggatc
    18121 cacacctgac gatgcggttc tgcgacgtga cctttcaatg ggttatgaaa ttccaccgga
    18181 cggacttcca gcggacgatc gcagatgaat ttatctttca gcactggcgg cagcaggtgc
    18241 gccagcgatt gggcgatttg tgtttccgaa ggcaggccat caggcgctgg cgcggacggc
    18301 attgtttttt gatgttcgaa acccgcttct ggtgcctgga aagaggccgt catataaaaa
    18361 atcggtttgc cgttttgaat agcagcaacc cggcgggcgc tgaagctgtt accgtcacgc
    18421 agcgtttcga catcataaat aatcggcttc ttactatcgc ctgggcgaag aaagtagctg
    18481 tgaaacgaat gtaccagccg ctcttcaggg acggtctctt ttgcagcata caaggcctga
    18541 cccacgacct ggccgccaaa cacctggcgt aaacctaaat cttcactctg gccgcgaaag
    18601 agtccttcct caattttttc cagatttaac aatgtcagta aattttttag cgcctgactc
    18661 atataactct ccagtaacaa agctgccgca gcaagccaaa gtgagttgag tataacgcaa
    18721 atttgccact ggtccgatgg gtgcaatggt ctgaattacg ggctaattac aggcagaaat
    18781 gcgtgatgtg tgccacactt gttgacgtta ctattttgtt aaccactctt ccggcgagga
    18841 aagttagccc gctggtgcat tgataataag gagaagtgaa tgaaactcgt gcacatggcc
    18901 agtggtttag cggttgcgat tgcgttggcg gcttgcgcag ataaaagcgc ggatattcag
    18961 acgccagcac cggctgcaaa tacgtctatt tcagcaacac aacctgctat ccagcaaccg
    19021 aatgtctccg gtaccgtctg gatccgtcag aaagtcgcac tgccgcctga tgctgtgctg
    19081 accgtgacac tttctgacgc gtcgttagcc gatgcgccgt caaaagtgct ggcgcagaaa
    19141 gcggtgcgta ccgaaggtaa acagtcacca ttcagctttg ttctgccatt taacccggca
    19201 gatgttcagc cgaacgcgcg tattctattg agtgcggcga ttaccgtgaa tgacaaactg
    19261 gtatttatta ccgataccgt tcagccggtg atcaatcagg gcggaactaa agccgacctg
    19321 acattggtgc cggtgcagca aaccgccgtg ccggttcagg ccagcggtgg cgcaacggct
    19381 accgtacctt cgacttcacc aactcaggtg aatccgtctt cggcagttcc agctcctacg
    19441 caatattaag cgcagttaac cctctccgcc cggagagggt tagtagttcc agcgataacg
    19501 ctgcaagtcg atttgcccgc ttcccgatac catcacacct tctgccagta atgcctgtcg
    19561 ttgacgctgt aaatccggtc cggttagcga aattgtgccg tggcgattaa ccacccggtg
    19621 ccagggcaag gtgctgcctt cggggagacg ctttaacaca ccgcctacct ggcgcgcggc
    19681 gcgcggcgat cccgccagtt tcgccacatc accgtaagtg gtgacatagc cttcgggaat
    19741 agcggcgacg atttgccaga cgcgttgggg aaatgaatct tctttttcca tcttttcttc
    19801 ctgaggtaat ttttcagcat aatctggaaa aacgcccgag tgaagccgca ttgcgcaaga
    19861 aaccagcatc tggcacgcaa tgggttgcaa ttagccgggg cagcagtgat aatgcgcctg
    19921 cgcgttggtt ctcaacgctc tcaatggggg ctctgttggt tctcccgcaa cgctactctg
    19981 tttaccaggt caggtccgga aggaagcagc caaggcagat gacgcgtgtg ccgggatgta
    20041 gctggcaggg cccccaccca tttctgcctc cccccgtttc gtcaaaaaat cccaacatgg
    20101 ctaaacttta accacaactg acgtcgcaag aattgtctgg ctgcgcagta cgcttcggag
    20161 gtatgtctga tgaagtatgt tgatggtttt gttgttgccg ttcctgccga taaaaaggat
    20221 gcctatcggg aaatggccgc taaggccgcg ccattattta aagagttcgg tgcgcttcgt
    20281 attgtcgaat gctgggccag cgatgtaccg gatggtaaag tgaccgattt tcgtatggcg
    20341 gtgaaagcgg aagagaatga agaggttgtc tttagctgga ttgaataccc ttcaaaagag
    20401 gtccgcgacg ctgctaatca aaagatgatg tcggacccac ggatgaaaga gttcggcgag
    20461 tccatgccgt ttgacggcaa gcgaatgatc tatggtggat tcgaatcaat catcgacgag
    20521 taggtagcgt gacgggctgt gcggcgtgcc gtcagcccta aaattcacaa atgttgctcg
    20581 gcccagcgta agaaatcctc tttcggtaat gccttgctgt agagccagcc ctgaccgtag
    20641 tgcacgccat gctggcgtaa ccactcttct tgtttactgg tttcgattcc ctccgctacc
    20701 attttcagtt tcagtgtttt tgccatttcg atgatatgcg gcgtgacatt tttatattcc
    20761 agcgcatcaa cgaaagattt atcgatcttc agaatgtcga catccagatc ctgtaaataa
    20821 cttaaacttg aataccccgt accaaaatca tcgagataaa tttcatggcc cgcctcccgg
    20881 tagcgagaaa ttatcggggc gctggttttc ggatcggcaa actcgcgttc agtgagttca
    20941 agcgcgatct gtctgggatt aacctgatag tgattgatca tttcacgcag caattgcggg
    21001 attttttccg aggtgagcac ggtggattca agattgatcg aaatatgctg ctgcggatgc
    21061 tgacgcagcc agtcgcccat atcttcgaag acgcttctta tgatcagtag cgtcaacggc
    21121 tcagaaaggc ccgtttgctg tgccagcgga ataaaactat ctggtgacaa ccaactaccg
    21181 tctgtctgcg gccagcgcgc cagtgcctca gcaccgacaa ttttgccatt ggctaaggag
    21241 acaatcggct gatagtgcac gcaaatatca cgattttcga tagcatcctg cagccgatga
    21301 tgcggtgact ggatacggcg caaaatacgc agcacaaaca tcgctgccag caggccaatc
    21361 accaacccga gcggtaacca aataaagact tgccgatgcc agcctttctg caacattttc
    21421 gttgaagacc aggtgatgat cgaaatattc atttccggga agggcaggat atcgtaaatg
    21481 attccattat tttcgatatg ctcaccgggt gttttttgta gtctggtaat aattccctga
    21541 gcaatttcat cgctgctggt tatcacaacg ttatgggcat tgccaataat ggcggcatca
    21601 atttgccatg agctataggg aatgacatca ataaaggaag cggggtcgat catgacgaca
    21661 taatgtgccg ttcccatggc aaccatgtaa cggataatgc ctaaatcgtt atgcgatgtt
    21721 aaccagacac gataaccatc tttcgaaatt ttacctggct cggggaaggt atcgggcggg
    21781 ctttcatgct ccagagacga acactgggga acgttgttat cgatataaac cacttcctga
    21841 atatagcgat aactgtaaga tacccgacgc atttccatga gatgggcttc gctacaggca
    21901 gcgccttgcc atctttccag ctcttgcagc gcatctttcc cttgtgtcgc caccttattg
    21961 gcccgaatag cgacgcggga ggaatacgta ttcagctctt caataaatga tgtttctacc
    22021 tgctgatggg ccagccagat gcttaagccg acaggcagca atactgaaag aatcagtact
    22081 cccgaaataa ggccgaccag atgtcgtgtt ctcaccagca tatccttatt aacacagtgt
    22141 agggaaagta tatctgattg tggaaaaagg ctggcaggag ataaggaggt agagccggga
    22201 tttcatgaag ggatgggtgg aaacgaacgt tgtcatttga ccaggcgcag agctttacgc
    22261 ctggtatgta accctaactt aagatgttga ttcggcgcga gcgagggtaa aaatatcgta
    22321 aaaagatagt tcacctttac ggacgatcat tttttgcagt tgttctttat gaacatccgc
    22381 gacacgcgga gagttcagga tggcttcaac tagcgcggca ggtacaaagc gcgtgttgta
    22441 ccattcgccg ttatagcaaa cgcggaaatc cagcacgtcg atatcttcat agcggtagcg
    22501 tgggtagaca tcgaaaaaga agaaaccatt aagcaggata aataatacaa ccagtagcca
    22561 gacagagcct gaaagcgttt cggactgcaa catcactgcc agggtggcaa aaaaagcaac
    22621 gtacataccg ataaacaacc ccggatgttt acggataaaa ctgatactaa agcggggttt
    22681 gttgtcgcgt ttttcgcggg tatttagaga ctcaattgtt tcggtcagca ggcgttgtat
    22741 ttcggtcatc gtttgccttc gtggtatctg caactttcca gggaacaccc tattttaacg
    22801 tgggaatagt agagggaaag taacagattt accacaattt tgcataacag ttgcgaaaaa
    22861 ccgattacaa ttttttaatt attctggctg gattaccgcc cacgacaacg ttgtccggga
    22921 catcttttgt gacaactgca cctgaggcta ccacgacgtt atcaccaatg gtcacaccag
    22981 ggttaatgac cgcacgtccg ccaatccaga cgttattacc gatggtgacg ggtttcccca
    23041 gttcagcacc gctattacgt gctacagggt cgatgggatg tgttgccgtg tagatatgaa
    23101 cgcctggtgc caacatacag ttatcaccaa tgcgaatagg gcagacatca agcatcacgc
    23161 aatcgaagtt ggcgaaaaaa ttattgccga gaaaaatgtt ataaccatag tcacagcgaa
    23221 acgttggctc aatataagcc tctgtcacct gaccgaataa atcagcaaga atttgctggc
    23281 gtaatgtgtg ctcttccgcc agggaatgat tgtatcggtg aataagccga cgagcgcgca
    23341 ggcgatcgcg agataacgcc tcatctgccg agtgatacaa ctcaccagca atcatctttt
    23401 ctttttctgt gctcattact gacctccggt tgctgaaaat gcggcaacac agtaagacga
    23461 aaagtcagtg aagagaacgt tcccattaca tctgtgtgat gaagatcaca tagggtatgc
    23521 cttcatggcg aaagaattat aaaactctta ataaacagcc ggttatagct ccgaaagcga
    23581 gttatttatt agcgaataaa tttccatact gaggaaggga tcttgtcgta cagtttattc
    23641 atggtcaatt cggcgaggcg gtgatctgcg gctgagtaaa ataccgccag ctcattatct
    23701 gataattcgt atttattttt ctcgataaca cgctccagcg tgtcaattgt ctggcaacga
    23761 cgtaaacgca ttaaataatc ggtttttgtt aaaggttttt cggacatact tctacccatg
    23821 gttgtaataa ttctaacaag ataaactcgc aggattctct ttcgtcgcat tgacaaaaca
    23881 acggaatagt cgattacctg acttccgcca tttctgaaga tcctgcatat taataccata
    23941 actactgaac aacataaagg tgtcatccag atattcgtcg atctgctcaa tgagcttatt
    24001 gtcttcatta tacttaattt tgtaattaag tgcgaaggtc gcaatatgct caatcagttc
    24061 attcaactgg aggttgatcg ccgaggttgg gtcgttaacc cagccatgat tgctttcttc
    24121 aaggtttgca agacagtcat gatacagggt ttcacagaga aacttaagct gcgcgatatc
    24181 atgtcttttg ggtgagtatt catccataac gcgtcccctt cttagcggtt gaactaacgg
    24241 acacctttcg ggatggaaaa aacttactga cctggacttg ccctctcgtt tgttagctta
    24301 actataagcc actctttgca ggttttcatc gcattattac gaaaaattac aattaggaag
    24361 gaagcgggac aggactcact ttttcatact agcactaacc cgcagcaggt aaaagcagtt
    24421 tgctcgaact gttcataaca tttcaggatt atcgccagat gaaattggta acactaatac
    24481 caggattgct ctgaatatga cgtaataacc gaggaatgaa taaagaatta ccgtaaataa
    24541 ttaagaatag cctctaaatg attatggatt attaaatcta ttagcgctac ttaatataaa
    24601 ttaactaaca atcagtattt tttatgaatt ttatacgtgt ttaatactgg ttttcgtatg
    24661 agatcctgag ttggtggttc aatgactcct taatgttcgt cggttatgca taaaaaaggc
    24721 cgcttgcgcg gccttagtga ttacacgttg tatcaatgat gatcgacagt atggctgtgc
    24781 tcgatatctt cattcttgcg gctaaagcgg cggcgaacca ccacaaagaa taccggaacg
    24841 aagaagattg ccagtaccgt tgcggtcacc atcccgccca ttacaccggt acctactgcg
    24901 ttctgcgcgc cggaaccagc accagtactg ataaccagcg gcataacgcc gaggataaac
    24961 gccaacgagg tcatcaggat tgggcgtaaa cgcatccgca ccgcatcaag cgtcgcttca
    25021 atcagacctt taccttcttt atccatcaag tctttggcga attcgacgat aagtatcgcg
    25081 ttcttcgccg acaaaccaat ggttgtgagc aggcctacct ggaagtaaac gtcattggtc
    25141 aggccacgga aggtggcagc cagcaacgca ccgataaccc ccagcggaac gaccagcata
    25201 acggagaacg gaatcgacca gctctcgtac agcgcagcca gacacaggaa cacgacaatc
    25261 aacgaaatcg cgtacagtga aggcgcctgg ttgccggaga gacgttcctg ataggacatt
    25321 cccgtccagt catagccaac accggtaggc agtttgctcg ccagttgttc catcagctcc
    25381 attgcttcac cggtactttt acccggtgcc gcctggccta agatttccat agatggcagg
    25441 ccgttgtaac gttccagacg cggcgaaccg tactcccaac gagaagagga gaacgccgag
    25501 aacggcacca tctgaccatc agcagcacga acataccagt cgccgatatc atccggcagc
    25561 atacggtatt tcgcttctga catgacgtaa actttcttca cacgaccgcg gtcgataaag
    25621 tcgttcacat agctaccgcc ccatgcagcg cccagagtgg tgttaatgtc gttgatagaa
    25681 acacccagcg cctgcgcttt ttcctggtcg atatcaatct taaactgcgg ggtatcttcc
    25741 agaccgtttg gacgtacgct ggtcaacata tcagggtgct tcgctgcttc tgcaagcaac
    25801 tggttacgcg cctgagtcag tttttcgtga ccaaggccag cctggtcaat cagctcaaag
    25861 tcaaagccgg ttgcggtacc cagttccacg attgcgggca ggttaaaggc gaaaaccatc
    25921 gcatctttga tttgcgagaa agcacgtgtt gcacgcatgg taatcgcttc aactttgttt
    25981 tcttcgcccg gacgatcggc ccagtccttc aaggaaacga acgcaatacc tgtattctga
    26041 ccacgtcccg caaagccgaa gccgttaacg gcgaacaccg actcaacgtt gttcttttct
    26101 ttggtcagat agtaatgcgt tacctcattg agcactttct gcgtacgttc ctgcgttgca
    26161 cctgctggca gctgaaccat ggtcataaat acgccctggt cttcatctgg caagaaggag
    26221 cttggcagac gcacgaacag ataggccatg ccgaccacga tgatcagata cagcaccagg
    26281 taacgccctg tactgcgcag aataccgcct acgctgtcgg tgtagtggtg cgtgctcttc
    26341 tcgaacatgc ggttaaacca gccgaagaag ccttttttac cttccccgtg atcgcctttg
    26401 gcaatcggtt tcagcatggt ggcacaaaga gccggagtca ggatcaacgc caccagtacc
    26461 gacagcgcca ttgctgaaac aatggtaata gagaactgac gatagatagc accagtagaa
    26521 ccgccaaaga aggccatcgg tacgaatacc gccgacagta ccatcgcgat accgaccaga
    26581 gcgccctgaa tctgccccat cgacttacgg gtagcttctt ttggcggcaa accttcttcc
    26641 gccataacac gctcaacgtt ttctaccaca acgatggcgt catccaccaa caggccgatg
    26701 gcgagcacca tcccgaacat tgttagcgtg tttatcgaga agccaaaggc ggcaaggacg
    26761 gcaaaggtcc ccagcaatac caccggtacg gcaatggtcg gaatcaacgt cgcgcggaag
    26821 ttctgcagga acagatacat aaccaggaac acgaggatga tcgcttcgac tagcgtttta
    26881 accacttcgt gaatcgagat tttcacgaac ggcgtggtgt cgtacgggta aacaattttc
    26941 agacccgacg ggaagaacgg ttccatcttc gccagttcag cacggattgc cgcagcggta
    27001 tccagcgcgt ttgcaccggt cgccagcttg atccccagac cggaagccgg ttggccgtta
    27061 aactctgcga tgatgtcgta gttctcacca cccagctcaa ttttcgccac atcacgcagc
    27121 agcacgcggg aaccatcctg attcactttc agcaggattt tgccgaactc ttcagtagag
    27181 gtcagacgcg tctgagcaat aatagaggcg ttaagctgtt ggcctttcac cggcggcgta
    27241 ccaccgagct gacccgccgc aacctgggcg ttctgcgctt tgatggcggt aatgacatca
    27301 accggcgtta gctggaattt gttcagttca ttcgggttca tccagatacg catcgcatac
    27361 tgtgaaccga acaactgaac gtcacccacg cccgacgtac ggctgatggc atctttcata
    27421 ttcgctgcca cgtagtcaga gatatcctcc tgcgtcatgg tgccatcggt gttgataacg
    27481 ccgacaacca tcaggaagct gctggatgat ttctcaacgc tcaccccttg ctgctgtact
    27541 tcttgcggca gcaacggcat cgccagctgc agtttgttct gcacctgaac ctgcgcgata
    27601 tccgcatcag taccagactc aaaggtcagg gtgatctgca cggtacccgt ggagtcactg
    27661 ttagaggaca tgtacatcag gttatcgata ccgttcatat tctgttcgat aacctgtgtc
    27721 accgtgtcct gcactgtttt cgcatcagcg ccagggtagg aggcggagat cgttactgcc
    27781 ggcggtgcaa tcgtaggata ttgcgccacc ggcagtttga ggatcgccag cccccctgcc
    27841 aacatgatga taatggcgat cacccacgca aaaatcgggc gatcgataaa gaaattaggc
    27901 atgtcttaac ggctcctgtt taagttaaga cttggactgt tcaggctgag caccgcttgc
    27961 ggcttgctgg ttattatcag cggtaacttc ttgtgctttt acctggacac caggacgcac
    28021 tttctgcagc ccacttatta ctacgcgatc gcctgctttc agaccttctg tcaccagcca
    28081 cttatcgccg atagcctggc ttgcaacgat cggacgggtt tccactttgt catccgcgcc
    28141 aaccaccagt acggtggcat cgccacgcgg cgtacgggtt acgccctgtt gcgggactaa
    28201 aatagcgttt ggattaagcc cttcttccag acgtgcccgc acgaacatac ccggcagcag
    28261 agtgtgatcc gggttcggga agatagcgcg tagggtgata gacccggtgg tctgatcaac
    28321 ggtaacgtca gagaattcca gcgtaccgtc ctgcgggaac ttaatgccgt cactggtgat
    28381 cagcgacact ttggctttgc cgttttcttg tttcaacgtg ccattcgcca gttcctgttt
    28441 caggcgcagg aagtcgttgc tggactgggt cacatcaacg tagatcggat caagttgctg
    28501 cacggttgcc agcgtagtcg cctgaccgtt ctgtaccaat gcgccttccg tcacgttcga
    28561 cttaccaatg cgaccgctaa tcggcgaggt gactttggtg taagccagat tgatccgcgc
    28621 agtttcaacg gcagctttcg ccgcagtgac cgcagcattc gcctgttgcg catcagccag
    28681 agcctgatcg tactcttgtt tactgatgta ctgagtaccg agcagtttct gataacgatt
    28741 caccgtcaat tgcgcgatat tggctgcagc ctgggctttc gccaaatcac ctttcgcact
    28801 gtcgtatgcc gcctgatagg tcgcaggatc aatctgatag agagagacac ctgcttcgat
    28861 gtcgctacct tctttgaaat tacgcttcag gataatcccg ctaacttgag gacgaacttc
    28921 tgcgatccgg taggcactgg tgcgacccgg aagctcggtt gtgatctgca gaggttcagt
    28981 tttgactgtt actacgccaa cggcgggcat ctgctggcca ccttgttggg cctgtttgtc
    29041 gtcacatcct gttagggcta agctgcctga gagcatcaga acgaccgcca gaggcgtaaa
    29101 ccctctgttt ttgttcatat gtaaacctcg agtgtccgat ttcaaattgg tcaatggtca
    29161 aaagttaata aacccattgc tgcgtttata ttatcgtcgt gctatggtac atacattcac
    29221 aaatgtatgt aaatctaacg cctgtaaatt cacgaacata tggcacgaaa aaccaaacaa
    29281 gaagcgcaag aaacgcgcca acacatcctc gatgtggctc tacgtctttt ctcacagcag
    29341 ggggtatcat ccacctcgct gggcgagatt gcaaaagcag ctggcgttac gcgcggtgca
    29401 atctactggc attttaaaga caagtcggat ttgttcagtg agatctggga actgtcagaa
    29461 tccaatattg gtgaactcga gcttgagtat caggcaaaat tccctggcga tccactctca
    29521 gtattaagag agatattaat tcatgttctt gaatccacgg tgacagaaga acggcgtcga
    29581 ttattgatgg agattatatt ccacaaatgc gaatttgtcg gagaaatggc tgttgtgcaa
    29641 caggcacaac gtaatctctg tctggaaagt tatgaccgta tagaacaaac gttaaaacat
    29701 tgtattgaag cgaaaatgtt gcctgcggat ttaatgacgc gtcgggcagc aattattatg
    29761 cgcggctata tttccggcct gatggaaaac tggctctttg ccccgcaatc ttttgatctt
    29821 aaaaaagaag cccgcgatta cgtcgccatc ttactggaga tgtatctcct gtgccccacg
    29881 cttcgtaatc ctgccactaa cgaataacct tgaatctgac tccaggattt ttcctggaca
    29941 ttttcgtcgt tgctattctg gttcactgcg tcgtgatatt cttgcggttt gactttttca
    30001 ggtcgttctt caggttcaga aaccttcatt catcatgact atgttccagt attacaaacg
    30061 atcacggcat tttgtttttt cagcatttat tgcttttgtt tttgtcttgt tatgccagaa
    30121 cacggcgttt gcgcgggcgt catcgaatgg tgatctgccg acaaaagcgg acctgcaggc
    30181 acaacttgac tcactgaata aacaaaaaga tctttctgct caggacaaac tggtgcagca
    30241 ggatctgaca gatacattag ctaccctcga taaaatcgat cgcgtaaaag aagagacagt
    30301 tcagctacgg caaaaggttg ctgaagcgcc ggaaaaaatg cgccaggcga ccgcggcgtt
    30361 aactgcactt agcgatgtcg ataatgacga agaaactcgc aaaattctga gtacgctttc
    30421 gttgcgccag ctggaaactc gcgttgccca ggcgctggac gatttgcaaa acgcacaaaa
    30481 cgatctggcg tcttataaca gccagctggt ttcgttacag acgcagcccg aacgcgtgca
    30541 aaatgcgatg tataacgctt cgcagcagct gcaacaaatt cgcagtcgtc tggatgggac
    30601 tgatgtcggc gagactgcct tacgtcccag ccagaaagtg ttaatgcagg ctcagcaggc
    30661 tttgctgaat gccgagattg accagcagcg taaaagcctg gaagggaaca cagtcttaca
    30721 ggataccttg caaaagcaac gggattacgt gacggcgaac agcgcccgtc tggagcacca
    30781 gttgcagctg ttgcaagaag cagtaaacag caagcgcttg actttaaccg aaaaaacggc
    30841 gcaggaagcc gtctccccgg atgaagccgc gcgtattcag gctaatccgc tggtgaagca
    30901 ggaactggaa attaaccagc agttaagtca gcgtctgatt accgcgactg aaaacggtaa
    30961 tcagttgatg cagcaaaaca ttaaagtcaa aaactggctg gagcgggcgc tgcaatcgga
    31021 acgcaatatt aaagagcaga ttgccgtcct gaagggcagc ctgctgttgt ctcgtatcct
    31081 ttaccagcaa caacaaacgc tgccctcggc ggatgaactg gaaaacatga ccaaccgcat
    31141 cgcggatttg cgtctcgaac agtttgaagt taaccagcag cgtgatgcac tcttccagag
    31201 cgatgcgttc gtcaacaaac tggaagaagg tcacaccaac gaagtcaaca gcgaagttca
    31261 cgatgcgtta ttgcaagtgg ttgatatgcg tcgcgaattg ctggatcagc ttaacaaaca
    31321 gttgggtaac cagctgatga tggccattaa cctgcaaatc aaccagcagc agttaatgag
    31381 tgtgtcgaaa aacctgaaat ccatcctgac tcagcaaatc ttttgggtga atagtaaccg
    31441 tccaatggac tgggactgga tcaaagcgtt cccgcaaagc ctgagagatg aatttaagtc
    31501 gatgaaaatc acggtgaact gggaaaaagc ctggcctgcc gtttttatcg ctttcctcgc
    31561 tggtttgccg ctgctgttga ttgccgggct gatccactgg cgtctgggct ggctgaaagc
    31621 gtatcaacaa aaactggctt ccgctgtggg ttccctgcgt aacgacagcc agctcaacac
    31681 accaaaagcg atccttatcg acctgatccg tgcgctgccg gtgtgcctga ttattctcgc
    31741 ggttggcctg attctgttga ccatgcagct caacatcagc gaactgctat ggtcgttcag
    31801 taaaaaactg gcgattttct ggctggtgtt tggcctgtgc tggaaagtgt tggagaaaaa
    31861 cggcgttgcc gtgcgtcact ttggtatgcc ggaacagcag accagccact ggcgtcggca
    31921 aattgtccgc atcagtctcg cgttgctgcc tatccatttc tggtctgtgg tggcagaact
    31981 ttccccgctg catctgatgg atgatgtgct ggggcaggcg atgattttct tcaacttgct
    32041 gctgattgcc ttcctggtgt ggccgatgtg ccgcgaaagc tggcgtgata aagagtcgca
    32101 caccatgcga ctggtcacca ttaccgtact gtcgattatc ccgattgcgt tgatggtgct
    32161 gaccgccacg ggttatttct acaccacgct gcgcctctcc gggcgttgga ttgaaaccgt
    32221 ttatttggtg atcatctgga acctgctgta ccagacggta ttgcgcggct taagcgtagc
    32281 ggcgcggcgt atcgcctggc gtcgtgcgct ggcgcgtcgg cagaatctgg tgaaagaggg
    32341 cgcagaaggt gctgaaccgc cggaagaacc caccattgca ctggagcagg ttaaccagca
    32401 gacgctgcgt attaccatgt tgctgatgtt tgcgctgttc ggtgtcatgt tctgggcaat
    32461 ttggtccgat ttgatcaccg tgttcagcta tctggacagc atcacgctct ggcattacaa
    32521 cggcactgaa gctggtgctg cggtggtgaa aaacgtcacc atgggcagtc tgttgtttgc
    32581 gattatcgcc tcaatggtgg cctgggcgtt gattcgcaac ctgcctggtt tgctggaagt
    32641 gttggtgctc tcgcgcctga acatgcgcca gggcgcgtcg tatgccatta ctaccatcct
    32701 taactacatc attattgccg ttggtgcgat gacggtgttc ggatcgctgg gcgtctcgtg
    32761 ggataaactc cagtggctgg cggcagcatt atccgtaggt cttggttttg gtttacaaga
    32821 gatcttcggt aacttcgttt ccggtttgat cattctgttc gaacgtccgg tgcgcatagg
    32881 cgacacggta accattggta gcttctcggg gacggtaagt aagatccgta ttcgtgcgac
    32941 aacgattacc gatttcgatc gcaaagaagt gatcatcccg aacaaagcgt ttgttaccga
    33001 gcgtctgatc aactggtcgt tgactgacac gactacgcgt ctggtgatcc gtctcggcgt
    33061 agcttatggc tccgatctgg aaaaagtgcg taaagtgttg ctgaaggcgg cgactgagca
    33121 cccaagggtg atgcacgaac caatgccgga agtcttcttt acggcatttg gtgccagcac
    33181 gttggatcat gagctgcgtc tgtatgtgcg tgaactgcgt gaccgtagcc gtactgtcga
    33241 tgagctgaac cgtactatcg atcagttgtg ccgtgaaaac gacatcaaca ttgcctttaa
    33301 ccagcttgaa gtgcatctgc acaacgagaa gggcgatgag gtgacggaag tcaaacgcga
    33361 ctacaaaggc gacgatccga cgccagcggt agggtaataa ccaaaggggg cgacatttag
    33421 ttgccccgag attactaaca aagtgcgcgt tgttcatgcc ggatgcggcg tgaccgcctt
    33481 atccggccta cgaaaccgca agaattcaat atattgcagg agcggtgtag gcctgataag
    33541 cgtagcgcat caggcagttt tgcgtttgcc cacaatctta ggggacattt agcggcccca
    33601 tttatttctc acttttccgc ctcatcatcg cgcgttaatt tcttttcata atcgcgtttt
    33661 acaatatcca gcgcgcgcag cacggtgcgg gctgggatct gattttcttc cagtagcaca
    33721 atcaaatcga cggccagttt gacatcgtca ggggcatttt ccagtgacat actctctcca
    33781 ttgctagcgg gttaaacgcg ctaacctgtt ttcgattttt tccagcgcat ggcggcagcg
    33841 tgccaggcgc gcttcatagg cttccacttc acgatgcagc gtctgctgtt ccacgagatc
    33901 ggtcgctcgt gccagacggg ctctacgttc ggcaaccatc tcacgtagcc gccgctcaaa
    33961 atcctgatgc tgaatacgtt tacgctgcca gcgggcaatt ttcggtggtg cgctatccca
    34021 ctcgcgtagc gaccaggctg tggcttcacg cgctatagcc tccagttgcg ccgccagatg
    34081 ttctgccagc caggcgactt gcggcagctg ttgctgctca accgcatgac gaagcgcagc
    34141 cagattatcg cccgcctcat cgagacaagc ctgcagtgtt gtcgcacgag tctgaaaaag
    34201 atgcctgtca aaacgggcgc ttagcgtggc aaattgcgct accggggcac aacgctgacg
    34261 cagcgtagcg agctgtcctt ccagtttttc cagcagcagg gcggttttca cgataagacc
    34321 tcaatgaaaa tgataattgt tatgctaaag tagcaactct gtaagctgac aacactcaat
    34381 atgcaacgaa tcattttaat catcattggc tggctggcgg tagtgctggg tacgctgggc
    34441 gtggtattac cggtattacc gacgacgccg tttatcctgc tggcggcctg gtgctttgcc
    34501 cgttcttccc cgcgctttca cgcctggttg ctgtaccgct catggtttgg cagctatcta
    34561 cgtttctggc agaaacatca tgcgatgccg cgcggcgtca aaccgcgggc gattttgctt
    34621 attttgctca cgtttgccat ttctctgtgg ttcgtccaga tgccatgggt gcgcatcatg
    34681 ttgctggtaa ttctcgcctg tttgcttttt tatatgtggc gaattccggt gattgatgaa
    34741 aagcaagaaa agcactgaag cacaacaatc gcagttgcaa ttattgcgta cagccagtac
    34801 attctggcgt tttcgagcac aggcgcaggc ggtcaaaggt taaacaactg ttacttttga
    34861 tacgtttaaa acgcgccgtg agtaccaccg taacaagcag gcatacactt atgaccgcga
    34921 ctgcacagca gcttgagtat ctcaaaaata gcatcaaaag cattcaggac tacccaaaac
    34981 ccggcattct tttccgtgat gtcaccagct tactggaaga cccgaaagct tacgctctca
    35041 gcatcgactt gctggttgag cgttacaaaa atgcgggcat taccaaagtt gtcggcaccg
    35101 aagcgcgtgg cttcttgttt ggcgctccgg tagctctggg tctgggcgtt ggctttgtac
    35161 cggtccgtaa accgggcaaa ctgccgcgtg aaaccatcag tgaaacttac gacctggaat
    35221 acggcaccga tcagctggaa atccacgttg atgccatcaa accgggcgac aaagttctgg
    35281 tggtggacga tctgctggca accggcggca ctatcgaagc gaccgttaaa ctgatccgtc
    35341 gtctgggtgg tgaagtggct gacgctgcgt tcattatcaa cctgttcgat ctcggcggcg
    35401 aacagcgtct cgaaaaacag ggcattacca gctacagcct tgtcccgttc ccgggccatt
    35461 aattatcgcc agtcttgtgc tgcccacgct acggacagca caagatgtgc attcagcctc
    35521 gccgttctga cggggctgtg ttagcattac cccttcgtga atccaccttc cagcgtttca
    35581 gagcctgcca atgagttatc aggtcttagc ccgaaaatgg cgcccacaaa cctttgctga
    35641 cgtcgtcggc caggaacatg tgctgaccgc actggcgaac ggcttgtcgt tagggcgtat
    35701 tcatcatgct tatctttttt ccggtacccg tggcgtcgga aaaacctcta tcgcccgact
    35761 gctggcgaag gggctaaact gcgaaaccgg cattaccgcg acgccgtgcg gcgtgtgcga
    35821 taactgtcgt gaaatcgagc aggggcgctt tgtcgatctg attgaaatcg acgccgcctc
    35881 gcgcactaaa gtcgaagata cccgcgacct gctggataac gttcagtacg ctccggcgcg
    35941 tggtcgtttc aaagtttatc tcatcgacga agtgcatatg ctgtcgcgtc acagctttaa
    36001 cgcactgtta aaaacccttg aagagccgcc agagcacgtt aagtttttgc tggcgacgac
    36061 cgatccgcag aaattgccgg tgacgatttt gtcacgctgt ctgcaatttc atctcaaggc
    36121 gctggatgtc gagcaaattc gccatcagct tgagcacatc ctcaacgaag aacatatcgc
    36181 tcacgagccg cgggcgctgc aattgttggc acgcgccgct gaaggcagcc tgcgagatgc
    36241 tttaagtctg accgaccagg cgattgccag cggtgacggc caggtttcaa cccaggcggt
    36301 cagtgcgatg ctgggtacgc ttgacgacga tcaggcgtta tcgctggttg aagcgatggt
    36361 cgaggccaac ggcgagcgcg taatggcgct aattaatgaa gctgctgccc gtggtatcga
    36421 gtgggaagcg ttgctggtgg aaatgctcgg tctgttgcat cgtattgcga tggtacaact
    36481 ttcgcctgct gctcttggca acgacatggc cgccattgag ctgcggatgc gtgaactggc
    36541 gcgcaccata ccgccgacgg atattcagct ttactatcag acgctgttga ttggtcgcaa
    36601 agaattaccg tatgcgccag accgccgtat gggtgttgag atgacgctgc tgcgcgcgct
    36661 ggcgttccat ccgcgtatgc ctctgcctga gccagaagtg ccacgccagt cctttgcgcc
    36721 cgttgcgcca acggcagtaa tgacgccaac acaggtgccg ccgcaaccgc aatcagcgcc
    36781 gcagcaggca ccgactgtac cgctcccgga aaccaccagc caggtgctgg cggcgcgcca
    36841 gcagttgcag cgcgtgcagg gagcaaccaa agcaaaaaag agtgaaccgg cagccgctac
    36901 ccgcgcgcgg ccggtgaata acgctgcgct ggaaagactg gcttcggtca ccgatcgcgt
    36961 tcaggcgcgc ccggtgccat cggcgctgga aaaagcgcca gccaaaaaag aagcgtatcg
    37021 ctggaaggcg accactccag tgatgcagca aaaagaagtg gtcgccacgc cgaaggcgct
    37081 gaaaaaagcg ctggaacatg aaaaaacgcc ggaactggcg gcgaagctgg cggcagaagc
    37141 cattgagcgc gacccgtggg cggctcaggt gagtcaactt tcgctaccaa aactggtcga
    37201 acaggtggcg ttaaatgcct ggaaagagga gagcgacaac tcagtatgtc tgcatttgcg
    37261 ctcctctcag cggcatttga acaaccgcgg cgcacagcaa aaactggctg aagcgttgag
    37321 cacgttaaaa ggttcaacgg ttgaactgac tatcgttgaa gatgataatc ccgcggtgcg
    37381 tacgccgctg gaatggcgtc aggcgatata cgaagaaaaa cttgcgcagg cgcgcgagtc
    37441 cattattgcg gataataata ttcagaccct gcgtcgattc ttcgatgcgg agctggatga
    37501 agaaagtatc cgccccattt gatcgtaagc acagcttacg ttcgtcatcc ttaacgtgat
    37561 tgagagagaa acctatgttt ggtaaaggcg gtctgggtaa cctgatgaag caagcccagc
    37621 agatgcaaga aaaaatgcag aaaatgcagg aagagatcgc gcagctggaa gtcaccggcg
    37681 aatctggcgc aggtctggta aaagtgacca tcaacggtgc acacaactgc cgtcgcgtag
    37741 agatcgaccc gagcctgctg gaagacgaca aagagatgct ggaagacctg gtggctgcag
    37801 cattcaacga cgcagcacgt cgtattgaag aaacgcagaa agaaaaaatg gcctctgtat
    37861 cctccggaat gcagctgccg cctggcttta agatgccgtt ctgatgcaaa ccagcccgct
    37921 gttaacacag cttatggaag cactgcgctg tctgccgggc gttggcccga agtcggcgca
    37981 gcgtatggcg ttcacgctgc ttcagcgcga tcgtagcggc gggatgcgtc tggcgcaggc
    38041 gctcacccgg gcgatgtcgg aaatcggcca ctgcgccgat tgccgcacct tcaccgaaca
    38101 ggaagtctgt aacatctgtt cgaatccacg tcgtcaggaa aacggtcaaa tctgcgtggt
    38161 ggagagtccg gcggacattt acgccattga gcagacgggg cagttttcag gtcgttattt
    38221 tgtgttgatg ggacacctgt caccgctgga cggcatcggt cctgatgata tcgggcttga
    38281 tcgtctggaa cagcgtctgg cagaggaaaa aatcactgaa gtgatcctcg ccaccaaccc
    38341 cacggttgaa ggtgaagcta ccgctaacta cattgccgag ctttgtgcgc aatatgacgt
    38401 ggaagccagc cgaatcgctc atggcgtacc ggttggcggc gaactggaaa tggtcgatgg
    38461 caccacgctg tcacattccc ttgccgggcg tcataagatt cgtttttaag caaacgagag
    38521 caggatcacc tgctctcgct tgaaattatt ctcccttgtc cccatctctc cctcatcctg
    38581 tttttaacct taaaatggca ttattgaggt agacctacat gaaaggacaa gaaactcgtg
    38641 gttttcagtc agaagtgaaa cagcttctgc acctgatgat ccattctctc tattccaata
    38701 aagaaatctt cctgcgtgag cttatctcta acgcctccga tgcggcggac aagctgcgtt
    38761 tccgcgcgct ttctaacccg gacctgtacg aaggtgatgg tgaactacgc gttcgtgtct
    38821 ctttcgataa agacaagcgc acgctgacca tctccgataa cggcgtgggg atgacccgcg
    38881 acgaagtgat tgaccatctg gggactatcg ctaaatccgg caccaaatca ttcctcgaat
    38941 ccctgggttc tgaccaggcg aaagacagcc agctgatcgg tcagtttggt gttggtttct
    39001 actctgcgtt tatcgtggct gacaaagtga ccgtgcgtac tcgcgcagca ggcgaaaaac
    39061 cagaaaatgg cgtcttctgg gaatcggctg gtgaaggtga atacactgtt gccgacatca
    39121 ccaaagaaga tcgtggtact gaaatcaccc tgcatctgcg tgaaggcgaa gacgagttcc
    39181 tcgatgactg gcgtgtgcgt tccatcatca gcaaatattc cgaccatatc gcgctgccgg
    39241 tagagatcga aaaacgcgaa gagaaagacg gcgaaaccgt tatctcctgg gagaaaatca
    39301 acaaagcgca ggcgctgtgg actcgtaaca agtcggaaat caccgatgaa gagtacaaag
    39361 agttctacaa acacatcgcc cacgacttta acgatccgct gacctggagc cacaaccgcg
    39421 ttgaaggtaa gcaggagtac accagcctgc tgtacattcc gtctcaggca ccgtgggata
    39481 tgtggaaccg cgatcataaa cacggtctga aactgtatgt tcagcgtgtg ttcatcatgg
    39541 acgacgcaga acagttcatg ccgaactatc tgcgcttcgt gcgtggtctg attgactcca
    39601 gcgatctgcc gctgaacgtt tcccgtgaaa tcctccagga cagcacggta acgcgtaacc
    39661 tgcgcaatgc gctgaccaag cgtgtgctgc aaatgctgga aaaactggcg aaagacgacg
    39721 cggaaaaata ccagaccttt tggcaacagt ttggcctggt actgaaagaa ggtccggcgg
    39781 aagatttcgc taaccaggaa gcgatcgcca aactgctgcg gtttgcgtct acccataccg
    39841 attcttctgc gcagaccgtg tctctggaag actacgtttc ccgcatgaaa gaagggcagg
    39901 agaaaatcta ctacatcact gcagacagct acgcggcagc gaagagcagc ccgcacctgg
    39961 aactgctgcg taagaaaggc atcgaagttc tgctgctttc cgaccgcatc gatgagtgga
    40021 tgatgaacta tctgactgag ttcgacggta aaccgttcca gtctgtgtct aaagttgacg
    40081 agtcgctgga aaaactggct gacgaagttg atgagagcgc gaaagaagcg gagaaagcac
    40141 tgactccgtt tatcgaccgt gtgaaagccc tgctcggcga gcgcgtgaaa gatgtccgtc
    40201 tgactcaccg tctgaccgat acgccagcga tcgtttcgac cgacgcggac gaaatgagca
    40261 ctcagatggc gaaactgttc gctgcggcgg gccagaaagt gccagaagtg aaatacatct
    40321 tcgaactgaa cccggatcac gtactggtga aacgtgcggc agatactgaa gatgaagcca
    40381 agttcagcga gtgggtagaa ctgctgctgg atcaggcgct gctggcagaa cgcggcacgc
    40441 tggaagatcc gaacctgttt attcgtcgta tgaaccagct gctggtttcc tgatgtcatg
    40501 ccggatgacc tttgtgtcat ccggcatttt tcttttcatc atctgcactt tccgcaaatt
    40561 atctcgccat taaccgtttc agccccaggt gcctttcttg aggcaatcgc ctgttggtgg
    40621 tatcgtttat cgctttttca aaaaattcga cacattttaa ggggattttc gcaatgcgta
    40681 tcattctgct tggcgctccg ggcgcgggga aagggactca ggctcagttc atcatggaga
    40741 aatatggtat tccgcaaatc tccactggcg atatgctgcg tgctgcggtc aaatctggct
    40801 ccgagctggg taaacaagca aaagacatta tggatgctgg caaactggtc accgacgaac
    40861 tggtgatcgc gctggttaaa gagcgcattg ctcaggaaga ctgccgcaac ggtttcctgt
    40921 tggacggctt cccgcgtacc attccgcagg cagacgcgat gaaagaagcg ggcatcaatg
    40981 ttgattacgt tctggaattc gacgtaccgg acgaactgat tgttgaccgt atcgtaggcc
    41041 gccgcgttca cgcgccgtct ggtcgtgttt atcacgttaa attcaatccg ccgaaagtag
    41101 aaggcaaaga cgacgttacc ggtgaagagc tgactacccg taaagatgat caggaagaga
    41161 ccgtacgtaa acgtctggtt gaataccatc agatgactgc accgctgatc ggctactact
    41221 ctaaagaagc ggaagcgggt aacaccaaat acgcgaaagt tgacggcacc aagccggtgg
    41281 ctgaagttcg cgcagctctg gaaaaaattc tcggctaatc aagccgcagc agatctgctt
    41341 tcgggcaggt ctgctcatcc tcctcaggca atacctctca cagcaattag ttcttcttct
    41401 tcacttttcc gctacaatta tcaacaagtt gaatcgataa gaggcggtaa tgcgtcagac
    41461 aaaaaccggt atcctgctgg caaacctggg tacgcccgat gcccccacac ctgaagcggt
    41521 aaaacgctat ctgaaacaat ttttaagcga cagacgtgtg gttgatacct cacggttgtt
    41581 atggtggcca ttgctgcgcg gcgtgatttt gccactgcgc tcgccgcgtg tggcgaagct
    41641 gtatgcctct gtctggatgg aaggtggctc gccgctgatg gtttacagcc gccagcaaca
    41701 gcaggcgctg gcacaacgtt taccggagac gcccgtagcg ctgggaatga gctacggctc
    41761 gccatcactg gaaagcgccg tagatgaact actggcagag catgtagatc atattgtggt
    41821 gctgccgctt tatccgcaat tctcctgttc aacggtcggt gcggtatggg atgaactggc
    41881 acgcattctg gcgcgcaaac gtagcattcc ggggatatcg tttattcgtg attacgccga
    41941 taaccacgat tatattaatg cactggcgaa cagcgtacgt gcttcttttg ccaaacatgg
    42001 cgaaccggat ctgctgttgc tctcttatca tggcattccc cagcgttatg cagatgaagg
    42061 cgatgattac ccgcaacgtt gtcgcacaac gactcgcgaa ctggcttccg cactggggat
    42121 ggcaccggaa aaagtgatga tgacctttca gtcgcgcttt gggcgggaac cctggctgat
    42181 gccttatacc gacgaaacgc tgaaaatgct cggagaaaaa ggtgtaggtc atattcaggt
    42241 gatgtgcccg ggctttgctg cggattgtct ggagacgctg gaagagattg ccgagcaaaa
    42301 ccgtgaggtc ttcctcggtg cgggcgggaa aaaatacgaa tatattccgg cacttaatgc
    42361 cacgccggaa catattgaaa tgatggctaa tcttgttgcc gcgtatcgct aaagctgagc
    42421 ggtaaagaac tgagcgccgt cgcgaagagc ctcgtcggcg gttttcatca tccgtgaata
    42481 atgcaaaaag gcgtgcagcg tgcctgggta gagtttgaac tcacagggct gctgatgcgc
    42541 cgctaacgtc tggtaaagca gacggctgtc atccagcagc ggatcgaact ccgccccggc
    42601 aataaaacag ggcggaactt cgcgagtgag atcattatta aacaggcagt aatacggcga
    42661 ctcgcggtcc gcgtcgttgc ttaaatacgc ctcttcatac atctgtaaat cctgttgcgt
    42721 taagccatcc cagacaccgc ccaacagacg acgagtcacg gaatcccgta atccgtaaag
    42781 cccataccac agcaaaacgc ccgcaacttt accgcaatcg atctgtttat cacgcaacca
    42841 caacgcactg gcgagcgcca acatggcacc tgcggaatca ccggtaaagc caatgcggga
    42901 catattgatt tgataatctt ccgcctgctg gtggaaataa caacaggcag ccacaatttc
    42961 ctctattgct tgcggaaaac gcgcttcagg tgaaagggtg taatcaatgc caatcaccgt
    43021 acattggctg tagcttgcca gcaggcgcat gatgcgatcg tgggtatcaa gattgccgag
    43081 aataaaaccg ccgccatgca aataaaatag cgtcgccggg ctatctggct gcggacaaaa
    43141 gagacgtgtt tccacctgcc catattttgt tggaaccatg taagctctgg ttgccatttc
    43201 tggagcgccc gcattccaga atcggcgctc aagcgtgtaa tactgtcgtt gctcagcaat
    43261 cgttcccgtt gcgggccagg gcggtaaatc cggctgaaga gtattcacaa cggtcttcat
    43321 ttcagcagaa ataaggtcca gaacgggtaa tttgttttcc ggcttcatgc aaaaactcct
    43381 ttcaaattac gtcattgtaa ggaaccactg ccatgaaaat gcgatcccgc ctgctgatat
    43441 tgaaactggc tgcgtctcgc gcgctcccgt cagattgtgt taacattcgc cgctcagtta
    43501 accacccgta aaaacaacca tgaaatttcc cggcaaacgt aaatccaaac attattttcc
    43561 cgtaaacgca cgcgatccgc tgcttcagca attccagcca gaaaacgaaa ccagcgctgc
    43621 ctgggtagtg ggtatcgatc aaacgctggt cgatattgaa gcgaaagtgg atgatgaatt
    43681 tattgagcgt tatggattaa gcgccgggca ttcactggtg attgaggatg acgtagccga
    43741 agcgctttat caggaactaa aacagaaaaa cctgattacc catcagtttg cgggtggcac
    43801 cattggtaac accatgcaca actactcggt gctcgcggac gaccgttcgg tgctgctggg
    43861 cgtcatgtgc agcaatattg aaattggcag ctatgcctat cgttacctgt gtaacacttc
    43921 cagccgtacc gatcttaact atctacaagg cgtggatggc ccgattggtc gttgctttac
    43981 gctgattggc gagtccgggg aacgtacctt tgctatcagc cccggccaca tgaaccagct
    44041 gcgggctgaa agtattccgg aagatgtgat tgccggagcc tcggctctgg ttctcacctc
    44101 ttatctggtg cgttgcaagc cgggtgaacc catgccggaa gcaaccatga aagctattga
    44161 gtacgcgaag aaatataacg taccggtggt gctgacgctg ggcaccaagt ttgtcattgc
    44221 cgagaatccg cagtggtggc agcaattcct caaagaccac gtctctatcc ttgcgatgaa
    44281 cgaagatgaa gccgaagcgt tgaccggaga aagcgatccg ttgttggcat ctgacaaggc
    44341 gctggactgg gttgatctgg tgctgtgtac cgcagggcca atcggcttgt atatggcggg
    44401 ctttaccgaa gacgaagcga aacgtaaaac ccagcatccg ctgctgccgg gtgctatagc
    44461 ggaatttaac cagtatgagt ttagccgcgc catgcgccac aaggattgcc agaatccgct
    44521 gcgtgtctat tcgcacattg cgccgtacat gggcgggccg gaaaaaatca tgaacactaa
    44581 tggagcaggg gatggcgcac tggcagcgtt gctgcatgac attaccgcca acagctacca
    44641 ccgtagcaac gtaccaaact ccagcaaaca taaattcacc tggttaactt attcatcgtt
    44701 agcgcaggtg tgtaaatatg ctaaccgtgt gagctatcag gtactgaacc agcattcacc
    44761 tcgtttaacg cgcggcttgc cggagcgtga agacagcctg gaagagtctt actgggatcg
    44821 ttaagttatc gaccagaccg gacgacgatg gcatccgtag gccggataag atgcgtcagc
    44881 atcgcatccg gcaatggaat caatgtttta ccccgtcacc acctcacccg ccggtggcgt
    44941 ttccagcagt tccagcatag tacgagcaat ttcacgctca cccatcacta cctgattcgc
    45001 accacgttcg gtgatatacg ccacttcatc gtcataatgg gcgcgggcaa taatctcaat
    45061 atccggattt ttcgcgcggg cagatgccac aatctcgccc gcttcataac cgttgggaat
    45121 cgtcaggatc agccattttg cacattccag atgcgccagt tgcataattt cttcgttcgc
    45181 cgcattgccc aatactgcgc ggaccccgcg ctctcgcagt tcatcaacac gggttcgtga
    45241 cgtctcaatc accaccagcg gaatatcaga ggcgagcaat ttctccccca gcaggctgcc
    45301 tacacgaccg taacctacca gtagcgcatg gttgcaaata tccactggga tctgcttctc
    45361 ttcttcgata gcctcttcca gcgtctgctc ttccagcgtt tcggtcttcg ccagatattt
    45421 ctccagcagc gcaaacagga ccgggttgag cataatcgac aaaatcgccc ctgccagtac
    45481 caggttctgc ccggcttgcg gcaatagatc cagcgccatg cccagtcccg ccaggataaa
    45541 cgcgaactca ccaatttgcg ccaggctggc ggcgatagtt aatgccgtac gttgggagtg
    45601 accaaacagt cgcaccagga aaaacgcggc taacgactta ccaaacagaa taatcgccag
    45661 cgtcgccagc actgccagcg gttgctgaat cagaattaac ggatcaaaca acatcccgac
    45721 ggagacaaaa aacagcaccg caaacgcgtc gcgcaatggc agcgtatcgt gggcggcacg
    45781 gtgactcagt tcagactcgt tcagtaccat cccggcaaag aacgcaccga gtgcaaagga
    45841 gacatcaaac agctccaccg caccaaaggc aatccctaac gccagcgcca gcaccgacag
    45901 ggtaaacagc tcacgggaac cggttgctgc gctacgtgcc ataatccacg gcaccagacg
    45961 gcgacctacc agcatcataa tggcgataaa tgcgatcacc ttgccgatgg tgatccccat
    46021 atcgacagca agagtggcaa agcccacatc gccttgttcc atcattcctg ccactgcggg
    46081 cagcaacacc agcgtcagaa ccatgaccag gtcttccaca atcaaccaac cgatggcgat
    46141 ttgcccacgc tgactgtcaa ttaattgccg ttcttcaagt gcgcgcagta acaccacggt
    46201 actggcggtg gaaaggcata agccgaacac gataccggtc attaacgacc agcccagcac
    46261 ggcggagagc gccataccca gcagcgtcgc cacggctatc tgggcgatcg caccgggaat
    46321 ggcgatggcc tttaccgcca tcaaatcctt cagcgaaaag tgcagaccca cgccaaacat
    46381 caacagaatg acgcccagtt cagccagttc cggcgcaagc ttggtatcgg caacaaagcc
    46441 cggagtgaat ggtcctgcca gcacacccgc taacagatat cccaccagag gagaaatacg
    46501 tagtttattg gccagcatgc cgaggataaa ggcgagcaca aggccgccaa caatggtggt
    46561 gataagcggg gtggcgtgat gcattccgtc tccttttcct ggtggttatt gtccattttt
    46621 ggccgggaaa accaaaatta caggtaatag tttatgacaa tttcattgat gatgtttatg
    46681 aataattgtt gaattttgca gaaaaatgga attagctgca aaaaaagcac ggatcggaaa
    46741 acagaaggcg gtttaacaga ggaaaggctt atggcgactg taaaacgatg cagccaaagt
    46801 ttgcctttgg ctgcaatgaa atcagtcttt atgccggtta tcaggcagga atatggtcaa
    46861 catccccaat agtggcagga aagcacagat tttatagact aactcgatgc tggtgtgatc
    46921 ggcgataagc cccagaactg ccgctcccag acctcccatg ccaaaagcaa aaccgaaaaa
    46981 gagtccagaa accataccga tacgtcccgg aagtagctcc tgagcgtaga ccagaatggc
    47041 agagaatgcc gaagcgagga taaatccaat aatcaccgtt aaaacccccg tccagtgcag
    47101 gctggcgtag ggtaaaatca gcgtaaacgg cgcaacgccg aggatagagc cccaaatcac
    47161 atatttccgt ccaattttat cccctacagg cccgccaatc accgtacctg ccgcaacggc
    47221 aaacaggaag gcaaacaaat gaagctgagc attctggata gataatccga atttttgcat
    47281 cagataaaag gtgtaatagc tgctgatgct cgccatatag aaatatttcg agaaaatgag
    47341 gattaacaga atgctgaccg ccagaacaac tttattgcgc ggcagtggat tgataatcgt
    47401 cgctttgggt tttcctttat tcattcggtg ctgtgccgag taccaacggc tgatttgcgc
    47461 caacaccacg atcgccagca gtgccgcaag cacaaaccag gcaacgttgc ctttgccata
    47521 aggcgcgata atcaccgccg ccagcaaggg gcccagggaa ctgccaaagt tgccgccgac
    47581 ctgaaagata gattgcgcca ggccatgacg cccgccggaa gccatacggg ccacgcgaga
    47641 agattccgga tgaaagaccg atgaaccggt accgaccagc gccgctgcca gcagaactgc
    47701 gccgaaactg cccgccagcg ccagcagcac cagaccactt aaggtaaagc acatgccaat
    47761 tggcaacgac cacggcatcg gatatttatc ggtccagtag ccgacaactg gttgcagtag
    47821 cgaagaggcg agctggaagg tgagggttat catgccaatc tgcataaatg tcagagaaaa
    47881 ctctgactga agcagcggat aaatcgccag aatcagcgat tggatcatgt cgttcagcag
    47941 atgtgagagg ctgatagcac ctaaaatacc aaacgatgtt cgggccttgg tcgttgacgc
    48001 agccgcgccc gccacaggct gggtttgttc actcattgcc ataggaaagt cactttttca
    48061 gggttgcgat gtaaagaatg atcttatttg tgattattac ccgactaaca tacctgtatg
    48121 cgccgcctga aggaagtctc aacgctgaat acagaatttc taatctggat acagatttat
    48181 cttcaccgga cgcagacttg tctatgatgt cgcgtcatac tatttttcaa cacgttgaaa
    48241 tcaggtcagg gagagaagta tgaaattatt gcagcggggc gtggcgttag cactgttaac
    48301 cacatttaca ctggcgagtg aagctgctct ggcgtatgag caggataaaa cctacaaaat
    48361 tacagttctg cataccaatg atcatcatgg gcatttttgg cgcaatgaat atggcgaata
    48421 tggtctggcg gcgcaaaaaa cgctggtgga tggtatccgc aaagaggttg cggctgaagg
    48481 cggtagcgtg ctgctacttt ccggtggcga cattaacact ggcgtgcccg agtccgactt
    48541 acaggatgcc gaacctgatt ttcgcggtat gaatctggtg ggctatgacg cgatggcgat
    48601 cggtaatcat gaatttgata acccgctcac cgtattacgc cagcaggaaa agtgggctaa
    48661 gttcccgttg ctttccgcca atatctacca gaaaagtacc ggcgagcgcc tgtttaaacc
    48721 atgggcgctg tttaagcgtc aggatctgaa aattgccgtt attggcctga cgacggatga
    48781 cacagccaaa attggtaatc cggaatattt cactgatatc gaatttcgta agcccgctga
    48841 tgaagcgaag ctggtgattc aggagctgca acagacagaa aagccagaca ttattatcgc
    48901 ggcgacccat atggggcatt acgacaatgg tgagcacggc tctaacgcac cgggcgatgt
    48961 ggagatggca cgcgcgctgc ctgccggatc gctggcgatg atcgtcggtg gccactcgca
    49021 agctccggtc tgcatggcgg cagaaaacaa aaaacaggtc gattacgtgc cgggtacgcc
    49081 atgcaaaccg gatcaacaaa acggcatctg gattgtgcag gcgcatgagt ggggcaaata
    49141 cgtgggacgg gctgattttg agtttcgtaa tggcgaaatg aaaatggtta actaccagct
    49201 gattccggtg aacctgaaga agaaagtgac ctgggaagac gggaaaagcg agcgcgtgct
    49261 gtacacccct gaaatcgctg aaaaccagca aatgatctcg ctgttatcac cgttccagaa
    49321 caaaggcaaa gcgcagctgg aagtgaaaat aggcgaaacc aatggtcgtc tggaaggcga
    49381 tcgtgacaaa gtacgttttg tacagaccaa tatggggcgg ttgattatgg cagcacaaat
    49441 ggatcgcact ggtgccgact ttgcggtgat gagcggaggc ggaattcgtg attctatcga
    49501 agcaggtgat atcagctata aaaacgtact gaaagtgcag ccattcggca atgtggtggt
    49561 gtatgccgac atgaccggta aagaggtgat cgattatttg accgccgtcg cgcagatgaa
    49621 gccagattca ggtgcctacc cgcaatttgc caacgttagc tttgtggcga aagacggtaa
    49681 actgaacaac ctgaaaatca aaggcgaacc ggtcgatccg gcgaaaactt accgcatggc
    49741 gacattaaac ttcaatgcca ccggcggtga tggctatccg cgccttgata acaaagcggg
    49801 ctatgtgaat accggcttta ttgatgccga agtgctgaaa gcgtatatcc agaaaagctc
    49861 gccgctggat gtgagtgttt atgaaccgaa aggtgaggtg agctggcagt aatccgaaag
    49921 tgccggatgt tcgcatccgg cacaatgctt aatcgcgacg ggcgatatca gcaaatttgg
    49981 catcgaggat ctttgccaga tcgcctgccg ccagttcgat atccagcccg cgcttgccgc
    50041 cggaaacata aatagtggca aattcttgtg cgggggcgtc gataatcgtt ggcagacgtt
    50101 ttttctgccc cagtgggcta atccccccta ccagataccc cgtcgaacgc tgcgcgacca
    50161 tcggatcggc catctcaact ttcttggcac ccagcgcttt tgctactttt ttaagatcca
    50221 gttgacctgc gaccggcgta acggccacgg caaggtgttt catatcaccg ttcactgcga
    50281 ccagcagcgt tttgtagacc tgatccggat tcaaacctaa ttttttgacg acttcatcgc
    50341 caaaattggt ttcagccgga tcgtgctcgt aggtgtggat ttgaaacgaa atcttgtttt
    50401 tttcgagtaa tttaactgcg ggtgtcatgg taattttcct gccttaaaca aaaaaacgaa
    50461 gcaagcatac gcctgatggc ggtccaaaaa aaagagtcat cttgcctaag agtattggca
    50521 ggatggtgag attgagcgac aatcgagtta caccgtcgct taaagtgacg gcataataat
    50581 aaaaaaatga aattcctctt tgacgggcca atagcgatat tggccatttt tttagcgcaa
    50641 catctgcggc aaattccctt ctccatacag gtgtagtgca ccaaccgcga ccacatagcg
    50701 ccccggcggc atggcgcata atttgtcccg ccaggcgaga tttcgctgat gcatcagcac
    50761 atcgtacagc gactgactga acgtattagg cagcgttatt tcattatttt gcggcggcgc
    50821 attcagccac cagctcatca tttgttgcag caaccgtgcg ttggtgtgcc agtgggtcag
    50881 cgtatcgtcc agcagcgcca gtccgttgtc agggagctgg agcaacatgg caatctggtt
    50941 ttcagcccct tccagttcaa tcacgggttt atgttgttgc ttagccgcct gcaatagctg
    51001 gtaatcgata ccgtattctg cccgcagccc cagtttttgc gcctgcgtcg cctgaagaac
    51061 catcgcgatt tgccacagcg gttgagtaga aaagagcgac ggagaaatgc ccatctcctg
    51121 gctaatatgc tgcaggtttt ggagttgctc ctcgctaatg cgcttttcca gggcctcgca
    51181 ggcaggcaaa ttggcaaaag gcgtatcgct ggtggaaaca tccgcctcga cgatcagcgc
    51241 atcggcgttt ttgagctttt tgagcaaacg ggtgggcagg ggagccatat cgtggctacc
    51301 catatgaata ctgccgatca gatgaaaatg gcgattgccg ggaagagaga tatcgatagc
    51361 aggccaggtg taatgattac cgcgcagcgc ggcccaaagc gtttttaccc ggtacaacag
    51421 atccatacga cctccctttg tgaaatatca tgctagcgcg cggggaggga tggcgcaaat
    51481 ggagttgatt gttgcacttg cgcacagtgc ttgtgtgcat tacaatgatt aaataatgga
    51541 caaggagatg agagaggaat ggctcaaaag aaaaatataa ggagttttcg tgatgcctgg
    51601 ttggctgatt tttttgtcca ttcaacgcca catcgaaaga tccctgctga aatccatacc
    51661 acgttatcac gaaagttgga catcattaat gctgccactt ctcatcggga tttacgctcc
    51721 cctccgggga accgatacga agagttatcc gggaaattac aggaatactc ctccattaga
    51781 gtgaataaac agtaccgact gatttttaaa tgggttaatg gcaaggccga agaattgttt
    51841 ttagacccac acaactacta aaacatagct gtcgtattga cgagaaaggt aaaactatga
    51901 aacaggcaac aagaaaaccg acgacacccg gagatattct tctctatgaa tatctggaac
    51961 cgctcgattt gaaaatcaat gagttagcag agttgctgca tgttcatcgt aatagcgtca
    52021 gtgcactgat caataacaat cgtaaactca ccactgagat ggcattccgt ctggcgaaag
    52081 tttttgatac cacagtcgat ttttggctaa acctccaggc ggcagttgat ctttgggaag
    52141 ttgaaaacaa catgcgtacc caggaagaat tgggacggat tgaaacagtg gctgaatatt
    52201 tggcacgccg tgaagagcgt gcaaaaaagg tcgcgtagaa taatttccct ataacggatg
    52261 cgccacccta aagcagtgca tccgcaatga tgtacttatt ccttcggttt aaaccgcagc
    52321 aaccggttgg cgttactcac tacggtaatc gacgagagcg ccattgccgc tccggcgacc
    52381 accgggttaa gcagcgtacc ggtgaacggc cacaaaatac cggcggcgac cggaatacca
    52441 atgctgttgt agataaacgc accgagcagg ttctgcttca tattgcgcag cgttgcgcgg
    52501 gaaatcgcga gcgcatccgc aacacccatc aggctatggc gcatcagggt aatcgccgcg
    52561 gtttcaatgg caacatcact gccgccaccc attgcaatgc cgacatccgc ctgagccagc
    52621 gctggcgcgt cgttaatgcc atcgcccacc attgccacct gacgtccttc actttgcaga
    52681 cgtttgatcg cttcggcttt accgtccggc agcaccccgg ctatcacctc atcaatccct
    52741 gcttctttgg cgatcgcatt ggcggtggtt gggttatccc cggttagcat caccagacga
    52801 tatcccgctt tatgcaggcg ttgcagcgcc gccacgctat cactacgcaa tggatcgcgt
    52861 acagccagca gggctaccgc tttcccgtca accgccagca gcacaggcgt tgccccttgc
    52921 gatgcctgag ctgaaatatc tgcttcaatc gctttagtat caacttgctg gtcatttaac
    52981 agcgcctgat tgcccagcaa taacgcatga ccttcagctt caccgctcac gcccagcccg
    53041 cgcaatgtgc ggaaaccgtt gacctgcggc agctgcatat cgcttgcttt atcgaggatc
    53101 gcccgtgcca gcggatggct ggaaccttgt tccagtgccg ccgccagacg cagcgcctgc
    53161 gcttcgtcaa aatcagcaaa tgtttttact gcgacaacct gcggcttccc ttcggtcagc
    53221 gtcccggttt tatcgaacac cacggtgtcg agcgtactgg cgcgttgcag cgcgtcggcg
    53281 tcccgcacca gcacgccaaa ctcagccgcc cgcccgacgc ccgaaataat cgacatcggc
    53341 gtcgccagtc ccagcgcaca cggacaggcg ataatcagca ccgtggtggc aatcaccagg
    53401 gtataaacaa tttgcggtgc cgggccaaag aaataccaga ttgccgcact gaccagcgca
    53461 atgactacca ccaccggcac aaacacagcg gagattttat ccgccagttg accgatttct
    53521 ggcttgctgc tctgggcctg tcgcaccatg cgaatgatcc gtgacagcgt agtatggctg
    53581 ccgaccgcac tggcgcgaaa cagcacactg ccgtcctgta ccactgtccc ggcatggacg
    53641 ctatcgcctt cgcctttttg ctgcgggatt ggttcgcccg tcagcatcgc ttcatccagc
    53701 cacgcttcgc cctgggtaat ttcaccatct accggcaccc gatcacccgt cgtcaggcgc
    53761 agcaacatac ctggctgaac atctgccagc ggcacgtttt tttcaccttc gttggtgacc
    53821 aggcgcgccg tcggtggggt taaatcgagt aatttctcca gcgccttaga agaacgctga
    53881 cgtgcgcgcg cttccagcat atgaccgaga ttaatcagac cgataatcat cgcactggct
    53941 tcatagtaaa gatgtcgcgc ttccatcggg aaccactgcg gccacaggtt gacgctcatt
    54001 gaatagagcc acgccacgcc cgtacccagc gccaccagcg tatccatcgt cgctgcaccg
    54061 ttcagcaggc ttttccatgc gctgcggtaa aaatggccgc cggcgaaaac cattaccgcc
    54121 agggttatca ggccgataac caaccacagg ctgcggttgt cggcggtgac catcatgtta
    54181 tcgccgatca tcccccagac catcaccggg atacccaccg ccagtgcgac aatcgcctgc
    54241 cagcggaagc gcttcatcgt agcgacggcg gtttcttgct ggcgctcgcg gcgtttagcg
    54301 tcatcttcaa tcgcttccgc gccgtagccc gctttttcca ccgcctgcac taaatcttgt
    54361 ggggaggcac tgcccatcac cagcgcagta cgctccgcca ggtttacccg tgcctgagtg
    54421 acgcccggta cgctttgcaa cgcattttgt acgcgggtga cacagctggc gcagctcatg
    54481 ccgctcagca gcaactgctg gctgtcatcg tcatcggcgg tcgctgccgg aagcgcctca
    54541 gaaaccgctg tcagtgcttc cgacgggatt gatgactccg ccagcggttt agcctttggg
    54601 tggcttacag atgcgtcata acccgcttgt ttgatggttt cgatcagctg ttctgcactg
    54661 gcagtcccgg taacgtgcgc ttcagtgaca gacacatccg cctgctcaac atccgggcgc
    54721 tgttcgagac tttctttcac gcgtttaacg cagtgaccgc aggacaggcc gtccagggtc
    54781 aggtcgatag tttgtgacat aaaacactcc ttttaagaca gttttgactg gctgtgataa
    54841 aggttaaacc ttccagcaag gggaaggtca agaaattaat aaaccaggcg ggtaaaagta
    54901 cgtaaagatt aaaaaatcgg ctcgatttgc atcaggatta gacatttatc tctttgtttt
    54961 cctgtagtta agttgcgggt gctaagttaa atcaggatgc ctgaaaattg gcaccggggt
    55021 gaggaattac ctcccgcatc tataaaaagg agttaacaaa agatgttaga tgcaaacaaa
    55081 ttacagcagg cagtggatca ggcttacacc caatttcact cacttaacgg cggacaaaat
    55141 gccgactaca ttccctttct ggcgaatgta cctggtcaac tggcagcagt ggctattgtg
    55201 acctgcgatg gcaacatcta tcgcgcgggt gatagtgatt accgctttgc gctggaatcc
    55261 atctcgaaag tctgtacgtt agcccttgcg ttagaagatg tcggcccgca ggcggtacag
    55321 gacaaaattg gcgctgaccc gaccggattg ccctttaact cagttatcgc cttagagttg
    55381 catggcggca aaccgctgtc gccactggta aatgctggcg ctattgccac caccagcctg
    55441 attaacgctg aaaatactga acaacgctgg cagcgaattt tacatatcca acaacaactg
    55501 gctggtgagc aggtagcgct ctctgacgaa gtcaaccagt cggaacaaac aaccaacttc
    55561 cataaccggg ccattgcctg gctgctgtac tccgccggat atctctattg tgatgcaatg
    55621 gaagcctgtg acgtgtacac ccgtcagtgc tccacgctca tcaatactgt tgaactggca
    55681 acgcttggcg cgacgctggc ggcgggtggt gtgaatccgc tgacgcataa acgcgttctt
    55741 caggccgaca acgtgccgta cattctggct gaaatgatga tggaagggct gtatggtcgc
    55801 tccggtgact gggcgtatcg cgttggttta ccgggcaaaa gcggtgtagg tggcggtatt
    55861 ctggcggtcg tccctggcgt gatgggaatt gccgcgttct caccaccgct ggacgaagaa
    55921 ggcaacagtg ttcgcggtca aaaaatggtg gcatcggtcg ctaagcaact cggctataac
    55981 gtgtttaagg gctgatcatg atgaacacgg aaggtaataa cgataacaaa cccctcggtc
    56041 tatggaacgt cgtttccatc ggtattgggg caatggtggg ggcggggatc ttcgcgctgc
    56101 tggggcaggc tgcgttgcta atggaagcct cgacctgggt cgcctttgct tttggcggta
    56161 ttgtggcgat gttttccggt tatgcctatg cgcgcctggg ggcgagctat cccagcaatg
    56221 gcggtattat cgacttcttt cgtcgcggat taggcaacgg cgtcttttcg ctggcactct
    56281 cgttattgta cctgttgacg ctggcggtga gcatcgccat ggtcgcccgt gcttttggcg
    56341 cttatgccgt gcagtttttg catgaaggca gtcaggagga gcaccttata ttgctctacg
    56401 cgttggggat cattgcggtg atgacgcttt tcaactcctt aagcaaccat gcggtagggc
    56461 ggctggaagt gatcctcgtc ggcattaaaa tgatgatcct gttattgctg atcattgccg
    56521 gtgtctggtc gctgcaaccg gcgcatattt ccgtctctgc gccccccagc tccggtgcgt
    56581 tcttctcctg tatcgggata accttccttg cctatgcggg ctttggcatg atggcgaacg
    56641 cggcggataa agtgaaagat ccgcaggtca ttatgccacg ggcgtttctg gtggcgattg
    56701 gcgttaccac gttgctttat atctcgctgg cgctggtttt gcttagcgat gtatcggcat
    56761 tagagttaga aaaatatgcc gataccgccg tagcgcaggc tgcttctccg ctgctcgggc
    56821 atgtgggtta tgtgatcgtc gtcatcggcg ctttactggc gacggcttca gccataaacg
    56881 cgaacctgtt cgccgtgttt aacatcatgg acaacatggg cagcgaacgc gaactgccga
    56941 agctaatgaa taaatccctg tggcggcaga gtacctgggg caacatcatt gtcgtggtgc
    57001 tgattatgct gatgacggcg gcgctgaatt taggctcact cgccagcgtt gccagcgcca
    57061 cctttttgat ctgctatctg gcggtgtttg tggtggcgat ccgcctgcgt catgatattc
    57121 acgcctcgtt gccgattctt atcgttggta cgttggttat gttgttggtg atcgttggct
    57181 ttatctacag tctctggtcc cagggcagcc gtgcgctgat atggattatt ggcgcactct
    57241 tactcagcct tattgtggca atggtcatga agcgcaataa aactgtataa catctctctg
    57301 tgcgcagtac ctcctgtatt attgtggtgg cggtcgatat tcgcactggc aaaaaaacgt
    57361 gcttgaatat ctgttgaaac cctttaacaa agcacaggag gcgttgcgcg aacgatgaac
    57421 attagcgatg tagcaaaaat taccggcctg accagcaaag ccattcgctt ctatgaagag
    57481 aaggggctgg tgacgccgcc gatgcgcagc gaaaacggtt atcgcaccta cacgcagcag
    57541 catctcaacg aactgacctt actgcgccag gcacgccagg tgggctttaa cctggaggag
    57601 agcggcgaac tggtgaatct gtttaacgat ccgcagcgtc acagcgccga cgtcaaacgt
    57661 cgtacgctgg agaaagtggc ggagatcgaa cagcatatcg aagaactgca atccatgcgt
    57721 aaccagctgc tggcgctggc gaatgcctgc cccggtgatg acagcgccga ctgcccgatt
    57781 atcgaaaatc tctccggctg ctgtcatcat cgggcggggt gattaaggct tgcgaaaata
    57841 gtcgtccatt attttcgtca gctccggacg ttccccgtca tcattgatgg caaaatccag
    57901 aaaaaaagca tctgcttgcg gcgttcggtt ttgcgccatc aaccggaagg tgagggggcc
    57961 tgaccaggtt tcggatgggc tgatgtgctt atcagggccg aaactctccc atgcgttggg
    58021 cggtaatgtt ttgcacgcgg tgagttttgg tatgtggtat tgcagctctg ttttgtgcag
    58081 acattccccg ccaggaaata ccagggtttg ttgctcatca ttaaaatggg ttgccgtgct
    58141 gagcaccagt gttttgagcg gtcgcccttc cagcgcggcg gcagtagcct tatggtgtcc
    58201 gtcgagcaga acgcagagat aggaatcagg gagataccag gccagagcgc gggcgattcc
    58261 agttttcgag agcagacaaa aaccgggcgg ctccctgttc accctccgca aaaccgctgt
    58321 agacaaattc cgcacaggta gggcaaccag cctcggacca gaaacgtatt aacgggacat
    58381 ccttgccagc agagcgaatg gataagaccg tgcgagtgcg gatcgtaaat cacacattga
    58441 cgcagaaatc ctttcccgga cttcaccgaa aaaatgacgc ccgcagcggt atttttcagc
    58501 agacgtactt caggttttcg aaaggggaaa aagcatggcc actccttagc caaaatttca
    58561 gttccctgaa ctgtgccaga aaattacgct ggtgactatt gctctggaaa tggaaacagt
    58621 gtccttaccc attaatcccc gtttgtaccg aaagaatatg ttgcaaactt tctaccataa
    58681 tgcgccccga ctgaaataag ccttcctgga tttcattgtt aacattcaca gcgtgaacca
    58741 gggaggctaa taacaccact tgcaagaagg caaaaataaa cgcaccgata atcataatat
    58801 gcgtcagggg gattttcctt tgctccttca ttttaaataa cccacaaact ggccttcttt
    58861 cattaaggcg acttttcctg tgtgatgata aacaatgaca ttatacagac ccgactgata
    58921 aaccgtggca tcggcattat cttcatctat ggcacataat gaatcattga gataagcaaa
    58981 attcttacta ctcattctaa aggtcatgcc aaatccttta taaatggctt tttttgccgc
    59041 ctgggcatgt tgtgcggctt ctgcattttt aaatgcctgc gcttcagaag cacttaaaat
    59101 agccgtctgg cgctgctgtt ctgcgagata acaggtgtca tggctgacgc cctgcgcctg
    59161 acaatctgcg atgcgggaag cgggtgagct acagccagcc agtaatataa ccgccagcag
    59221 ggaaaaggat ttaatggagc tattcatcat tgttaacctt aaataatgtg cgaattaatg
    59281 aagaggtgaa acccggcaag cttccttgct cttatttata ttgaggattg aaatattaga
    59341 acgaatatcc tacgccgaca cctgcgccga aatcagattg cgtatccgca gaaataccgg
    59401 ctttaagtac cgtatgcgtt ccggcattaa aagatgttcc gacagacacc gcagattcac
    59461 cgttgaaggt tcccgctccc gctgacacca tcatcacgtc accggtttta acctgtggtt
    59521 gtgaggcgat agcgatggca gatgcagttc ccgcattggc ttctttacgg ttgtcctcaa
    59581 cttcgttttt cagcgaactg taatggttat tgagcgtgtt aatttgttgg ctgttatctg
    59641 cgacccgttt ttgcgtgttg cttaactgtt gctgcgtttg tgcggaatct tgtctcagac
    59701 tttcaatttg ctcatgtgcc tggacagact tctgcgccag actttgcatt tgtttatcgt
    59761 attgagccct ttctttttgc tgttgtgctg ccacatcggt tgtcagtgca ttcattttct
    59821 gcccttgttc cgtggtagtg cgctgattag cctcattttg agccgccagt ctggcattaa
    59881 tcatctcctg ataatgcgca gtattttgcg tatctccagt cgcggcgagc gttttttgtg
    59941 tggctacgat ctgcgcctgt tgctctgtaa tttgcgtttg ctgggcatca gttaccgcag
    60001 actgtcgggc gttgatcatg ctctgataac gggcaggtgt ctgtgtatca cctgtagcgg
    60061 caacggtttt ttgtcctgcg tcaggatctg tctggacagt gggagtctga gtggtatcag
    60121 gtgtttcaga agggatgtag tgatgttcag tcaaataggt atacgcagcg gggctgttgt
    60181 ctgtgcgttc taaaaagcgg gtaatcttct gctcaatctc cgattgcgac atgccagagt
    60241 taatggctga aagctctgtt gccgccgctt caatggtatc accagctaaa acaacagggc
    60301 tggatgttgc tgaaattatg agtgccagta aagctacgtt tacagttttc attcattttc
    60361 tcttgttcaa aataagtcgc attaatgttt ctataagaaa caaataatat tattttaaga
    60421 aataaactgc gttgcaattt aatcgaaatc tacgccgcca tttgtgtgat gtaaagcgca
    60481 tttttgtctg gtgtaagcgt aaacaccacc aataagaaac aaaaaatgtt tcttatataa
    60541 ataaaatcga aaatcatttt gtgctctttc aatgtaaaaa agagtaaaga cttgttttga
    60601 taagcgaaat aaattctgtt tctggactaa acgtgaagcg gttcctgatg tgtgctaaaa
    60661 tgcgtttctt tgataaatct caatttttaa gaggtttacg ccaggcagga aaatttaaat
    60721 gaatgaattt gagaagattt ttaatgaaat gaatctggac cgggcattgt taccgatcct
    60781 ttttcgttcg aaccggtcga cagtgtggaa gtatttaagc ggcgatagta cggctcctgc
    60841 ctctgccatg tcgctgatca tgctgttgca gttgatccag aaacgaaacc ccgatctgtt
    60901 agccgaatgg ttaacgcttt ctgatttcac cattccgcca gaagtttacc tggaccaacc
    60961 tgattactgg aaagggtggg tgtataccca acacaaggtc aataaaaacg tcctggaata
    61021 tctaaaaaaa cactatccgg atgaagacca aaaaagtatg ggtaaaggcc gggaagagta
    61081 attttgcctc tactcggcta tatgccggat gcggcgcaag cgccttatct ggcctacgat
    61141 tcgggcatat tttgtaggca tgataagacg cgccagcgtc gcatcaggcg atgaccgcac
    61201 ggataatcag cgttatccct tctatcgcaa tcacctcaac atgcgtacct gcactgagat
    61261 cctcgctggc gctgacaggc catgaactgt caccgacgcg catatgacca cgcccgttga
    61321 ccagcggaga ttccagaaca aaacgtcggc caatcaactg ctgcccgcgc tggtttaaat
    61381 gactgtcgct gtgcttttgt tcgcgcaccc gccgcgacaa ccatttccac cacagccagg
    61441 cggcgagcag cgtcaggacg gcaaacatta ccccttgcca ctcccaaccc agcggcagca
    61501 accagaccac cagaccggta atcaccgctg ccacgccgct ccacaacaga taaccattgc
    61561 cgcccaacat ctcggctgcc agcagcaaac cgccgagact tagccagaaa atatgtggat
    61621 gaacgaccat taactccatc atggctgagt ccgtttgttg gcgctgtctt tcaccagctc
    61681 agcaatcccg gcaatcgacc ccatcaggct gctggcctct aatggcatca tcaccacttt
    61741 gctgttactg gacgaaccga tctgctgtaa cgcttcggtg tatttctgtg cgacgaagta
    61801 gttcaccgcc tgaatatcac cggaggcaat ggcttcagac accattttag tggcgcgggc
    61861 ttctgcttca gcggaacgtt cacgcgcttc agcctgtaaa aacgccgact gacgttcgcc
    61921 ttccgctttc aggatttgcg actgtttttc accttcggct ttgaggattt ccgcctgacg
    61981 gatcccttcc gcttcaagga tgtaagcgcg tttggtacgt tccgctttca tctgcgcgtt
    62041 cattgaagag ataagctctg ccggtgggcg cacgtcgcga atttcaatac gggtgacttt
    62101 aatcccccac gggttggtgg cctcatcgac aatacgcagc aggcgtgagt tgatgctgtc
    62161 gcgctgagag agcatttcgt caagttccat tgaacccagc acggtacgga tgttagtcat
    62221 ggtcaggttg atgatcgcca gctccagatt gctgacttca taagccgcgc gcggcgcgtc
    62281 aatcacctga ataaagcaca cggcgtcgat ggtaacgttg gcgttatctt tcgagataac
    62341 ttcctgggaa gggatatcga gcacttgctc catcatattg atcttgcgac caatgcgatc
    62401 cataaacggc accaccagac tgagccccgg ctgtaacgtt ttggtatagc gaccaaaacg
    62461 ttctactgtc cactgatagc cctgcggcac gattttgacg cccgcgccga caatgaccag
    62521 cgcgacaaaa atgagaatcg ggataaagat aagcatcgga aaaacctcct gttgtaccgt
    62581 ccataatcag caaaattgct gcttgattaa acaaattata cctgattact gaaagagagt
    62641 tcccccttat tcctgcgaag gataaactgt ttttagtaaa aatcagaaaa agggaacagt
    62701 gatgcaggaa aatagtcctt tgcttcagct acaaaacgta ggatatctgg cgggtgatgc
    62761 gaagattctt aataacatca atttttcgct gcgtgctggc gaatttaagt taattaccgg
    62821 tccttctggt tgtggcaaaa gtacgctgct aaaaatagtt gcttcattga tcagcccaac
    62881 cagcggaacg ttactgtttg aaggtgagga tgtcagcaca ctaaagccag aaatctaccg
    62941 ccagcaagtc tcttattgcg cccagacgcc gacgctgttt ggcgacacgg tatacgataa
    63001 tctgatcttt ccctggcaga tccgtaacca gcagcctgac ccagccattt ttctcgattt
    63061 tctcgaacgc ttcgccttac cggacaccat tttgacgaag aatatcgccg agctatctgg
    63121 tggtgaaaaa caacgcatct cattgattcg taacctgcaa tttatgccga aggttttatt
    63181 gctggatgaa ataaccagtg cgctggacga aagtaataaa cataacgtta atgagatgat
    63241 ccaccgttat gtgcgcgagc aaaatattgc cgtgctgtgg gtgacacacg ataaagacga
    63301 aattaatcat gcggataaag tgattacact gcaaccgcat gccggagaaa tgcaggaagc
    63361 acgctatgaa ctcgcataat attactaacg aatcattagc actggcattg atgctggtgg
    63421 tggtggcaat cttaattagc cataaagaaa aactggcgct ggagaaagat attctctgga
    63481 gcgtcgggcg cgcgataatc cagctgatta ttgtcggcta tgtgctgaag tatattttca
    63541 gcgtggatga tgccagcctg acattattga tggtgttatt tatctgcttt aatgcggcgt
    63601 ggaacgcgca aaaacgcagt aaatatattg ctaaagcttt tatctcatcg tttattgcca
    63661 ttacggtcgg ggcgggaatt accctggcgg tgctgattct ctcagggtcg attgaattta
    63721 tcccgatgca ggtgatccct atcgccggga tgatagccgg caacgccatg gtagcggtgg
    63781 ggttgtgtta caacaattta gggcaacggg tcattagcga acagcaacag attcaggaga
    63841 aactgagtct tggcgcgacg ccgaaacagg cttcagcgat attgattcgc gacagtattc
    63901 gcgcggcttt aattccgacg gtcgattcag caaaaacggt aggcttagtg agtttaccag
    63961 gaatgatgtc cgggctgata tttgccggga ttgatccggt gaaggcgatt aaatatcaga
    64021 ttatggtgac ctttatgctg ctctcaaccg ccagcttgtc gaccattatt gcctgctatt
    64081 taacctatcg taagttttac aattcgcgcc accaattggt ggtgacgcaa ttgaagaaga
    64141 aatgatgcga tgccggacgc gcagcatcgc atccggcgtt gtggttcatg tgccgatcaa
    64201 cggaaactca atacaacaac gcgtacagct ggcggcgata cttcgacgcc agtgcatcac
    64261 ccgtacccag cgcagcgagg atctcctgga atgttttacg tgtctgacca tctgcggcgg
    64321 tgagatcttt acgcagatgc ccaaacagta actccagcgc ctcttcattg cgcccaacct
    64381 ggtgcagttg cagtgccagt tgtgtcgcca gtgcggcatc ttctggattc tccgccacct
    64441 gctgttgcaa ctgttgaatt tccggcgtat cagccgcctg cttcagcagt tcgatttgcg
    64501 ccaccagccc ctgatagcgg gtgtcctgat cctgcaacgg aatggttttc agcacggctt
    64561 ctgcatcttc agaacggttc agcgcaatca gcgtttctgc cagcagcagg ccgatctccc
    64621 cgttctgatt cgacaactgc caggcgtctt tcagcaatgg caaggcgtcg gtgtaattgc
    64681 cttcctgcat cagctgcatc gcctgctgcg ctttcagctc ttcttcgcgc ggcagcactt
    64741 tatccagcag ggcgcggatc gcctcttccg gttgcggccc ctggaagcca tctaccggtt
    64801 gcccgttctg gaacagatac acggtcggaa tcgcgcgcag accaaactgg gcggcaatca
    64861 tctgctccgc gtcgcagtcc agcttcgcca gaataaattg cccgttgtac tgcgccgcga
    64921 ggctttccag gatcggggtt aactgcaaac agtgctggct acgttcagac caaaaataga
    64981 acagcaccgg agtggtcatc gactgttcaa gaacctgttg caggttagat tcgttaatgt
    65041 tgacaatatt ttctacggac atggagtcgc tctctgttgt cgatattttc tttgacatgg
    65101 gggcttaagc gcgcgcttca actcacccct gcaatatttt gtccatcacg cgccccggca
    65161 gcaggcgctt aagcaccatt accgcccagg tcaccagcgt taccggatag cgcatcttcg
    65221 gcttctcgct aataaaagca tggcgtactt tgtccaccac cgcttccggt cccaacgtaa
    65281 agcgggcggc gatgccggga ttttcgactg gtttatcact ttgcgtctgg ttgacgttgt
    65341 cggtgaagcg agtacgaatg ggaccgggtt cgatcaggct gactttaatt ccacagtggc
    65401 gtagctccat gcgcagcgcg tctgaccacg cctccagcgc atatttactg gccgcgtaag
    65461 caccacgacc cggcgtggag attaatccca tcaccgatga ggtcatcaca atacgccctt
    65521 caccgtgcgg taacatcgcg ggtaacaggc gcatggtgag ctggtgtgcg ccgaaaaagt
    65581 tggcggaaaa ctgctgttcc atctgcgcac ggctgatggt ggaaaggggg ccatacatgc
    65641 cgaatccggc attgttaaag atcccataca gacaattatc ggtcagggcg atcacctcgt
    65701 cggctgcgcg atcaacactt tctggtgaat ccagatcgat caacacgccg gtaaatccca
    65761 tgctgttcat gcgctcaaca tcatccggtt tccggcaacc tgccagcaca tgaaaaccct
    65821 ggcgttttaa ttcgagcgcg ctttccaggc caattccact ggaacatccg gtaattaaga
    65881 ccgatttttg cataacttta cctgtcagga tctccgttgc tttatgagtc atgatttact
    65941 aaaggctgca actgcttcgc catccagtcg gcaataaacg gctgggcgtc gcggttggga
    66001 tgaataccgt catcctgcat ccattgtggt ttgaggtaga cctcttccat aaaaaagggc
    66061 agcagcggaa catcaaactc tttggcgagt ttggggtaaa tggcgctaaa ggcttcatta
    66121 taacggcgac catagtttgc aggcagacgt atttgcatta acaatggttc agcgttggcg
    66181 gctttgacat cctgcaatat ctggcgcagc gtttgctcgg tttgttgtgg ctgaaaacca
    66241 cgcaaaccgt cattgccgcc cagttctacc agcacccagc gcggctgatg ctgtttcagc
    66301 agagccggaa ggcgcgccag cccttgttgc gaggtgtcgc cgctgatgct ggcattgact
    66361 accgacgttt tactctgcca cttatcgttc aacaaggcag gccaggccgc gctggcagac
    66421 attcgatacc cggcgctcag gctatcaccc agaatcaata acgtgtccgc tgcggcggca
    66481 cggaaggtta acaggaccag gaacaggaag ggcaaatgcc agcggaaaac attgttgaag
    66541 ttcatcatct taagaagtcc gtcggtcagg gggagcatga actctccatc ctcaccggag
    66601 ttgagctggt tgtcaaacgt ggcgagacca tcgcactggt gggcgagtcg ggatcgggta
    66661 agtcaacttt gctggcgatc ctcgccgggc tggatgacgg cagcagtggc gaagtgagtc
    66721 tggtgggaca accgctacat aatatggacg aagaagcgcg ggcaaagttg cgcgcgaagc
    66781 acgtcggctt tgtttttcag tcatttatgt taattcctac ccttaacgcg ctggaaaacg
    66841 tcgagcttcc ggcgctgctg cgcggtgaga gtagcgcgga aagtcgtaac ggggcgaaag
    66901 cgttgctcga acagttaggg ctgggtaaac gtctggatca tcttccggca cagctttccg
    66961 gcggtgaaca gcaacgagtg gcgctggcac gagcctttaa cggtcgacct gatgtgctgt
    67021 ttgccgacga acccaccggc aatcttgacc gacagacggg cgataaaatt gccgacctgc
    67081 tattttccct caaccgtgag catggcacca cgttgattat ggtgacccac gacctgcaac
    67141 tggcggcacg ctgcgaccgc tgcttacggc tggtgaacgg gcaattgcag gaggaagcat
    67201 gattgcacgt tggttctggc gcgaatggcg ctcgccgtcg ctattaattg tctggctggc
    67261 gctaagcctg gcggtggcct gcgtgctggc gctgggcaat atcagcgatc gcatggagaa
    67321 gggcttaagc cagcaaagcc gggagtttat ggcgggcgat cgggcgttgc gcagttcacg
    67381 cgaagtgcca caagcgtggc tggaggaagc gcaaaagcgc ggcctgaaag tcggcaagca
    67441 gctgactttc gccacaatga cctttgcagg cgacacaccg cagctggcga acgtcaaagc
    67501 ggtggatgat atctacccga tgtatggcga tctgcaaact aatccccctg gcctgaaacc
    67561 gcaggcgggc agcgtattgc tggccccacg cctgatggca ctgcttaacc tgaaaacagg
    67621 cgacaccatc gacgtgggtg atgccacctt gcggattgcc ggagaagtga ttcaggaacc
    67681 ggattccggt tttaacccct tccagatggc tccgcgtctg atgatgaatc tggcggatgt
    67741 cgataaaacc ggagccgtgc agccggggag tcgggtcacc tggcgctata aattcggcgg
    67801 cagcgagaac cagctcgacg gctatgagaa atggttgtta ccccagctta aacctgaaca
    67861 acgctggtac ggtctggaac aggacgaagg cgcgctgggg cgatcgatag aacgttcgca
    67921 acagttcctg ctgctttcgg cgcttctgac cttgctgctg gcggtggcgg cggttgcggt
    67981 ggcgatgaat cattactgtc gcagtcgcta cgacttggtg gcgatcctca agacgctggg
    68041 ggcagggcga gcgcaactgc gtaagttaat cgtcggtcag tggttgatgg tgctggcgct
    68101 ttcagccgtt accggtggtg cgataggcct gttgttcgaa aacgtgttga tggtgctgct
    68161 caagcccgtt ttgcctgctg cactaccgcc agccagcctc tggccgtggt tgtgggcgct
    68221 gggcaccatg acggtcatct cgctgctggt ggggctacga ccgtaccgct tgttgctggc
    68281 aacgcagcct ttacgcgtat tacgtaatga tgtggtcgcg aacgtctggc cgctgaagtt
    68341 ttatctgccg attgtcagtg tggtggttgt gctgctgctc gccggattaa tgggtggcag
    68401 catgctgctt tgggcggtgc tggcgggcgc ggtagtactg gctttgctgt gcggtgtgct
    68461 gggctggatg ctgctgaatg tacttcgccg catgacgctg aaatcgctac ctctgcgcct
    68521 ggcggttagc cgcctgttac gtcagccgtg gtcaacgtta agccagcttt cggcattttc
    68581 gctctccttt atgctgctgg cgctgttgct ggtattgcgt ggcgatctgc tcgaccgctg
    68641 gcagcagcag ctacctccag aaagcccgaa ctacttttta attaacatcg ccacagaaca
    68701 ggtagcaccg ctaaaagcgt tcctcgcgga acatcagata gtcccggaat cgttttatcc
    68761 ggtggtgcgg gcgcggctga cggcgattaa cgataagccg acagaaggca atgaagatga
    68821 ggcgctcaat cgcgaactca atcttacctg gcaaaatacg cggcccgatc ataatccgat
    68881 tgtcgccggt aactggccgc caaaagccga tgaagtgtcg atggaagagg ggctggcgaa
    68941 acgcttaaac gttgccctcg gtgataccgt gacttttatg ggcgataccc aggagttccg
    69001 cgctaaagtg accagcctgc gcaaagtgga ctgggaaagt ctgcggccta atttctattt
    69061 tattttccct gaaggggcat tagacgggca accgcagagc tggcttacca gtttccgctg
    69121 ggagaatggc aacggcatgt tgacgcaact caaccgccag ttccctacta ttagcctgtt
    69181 agacattggc gcgattttaa aacaggtcgg tcaggtgctg gagcaggtaa gtcgggcgct
    69241 ggaggtgatg gtggtgctgg tcaccgcctg cggtatgttg ctgttgctgg cacaggtgca
    69301 ggtgggaatg cgtcagcgtc atcaggagct ggtggtgtgg cgcacactcg gtgcggggaa
    69361 aaaactgctg cgtaccacgt tgtggtgtga gttcgccatg cttgggtttg tttctggcct
    69421 ggtagccgca attggtgcgg aaacggcgct ggcagtgttg caggcgaaag tgtttgattt
    69481 tccgtgggag ccagactggc gattgtggat tgtgctgccg tgcagcggtg cactgctgct
    69541 gtcgcttttc ggcggctggc tgggtgcgcg actggttaag ggtaaggcgc tgttcaggca
    69601 gtttgcggga tgatgagagt aaataagggg cgttctggtt gaatggaacg ccttgtttaa
    69661 attaattcta caatatgttt cattgatgat gttattgaat tggtgatttc ctatctttct
    69721 attgctgata ttaatctgaa tcggtggagt ttaatgattt taaatatagc cctacaaaaa
    69781 taactcagac taataaaatc ataaatcata t
//
LOCUS       DAZGQL010000012        69192 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.012, whole genome shotgun sequence.
ACCESSION   DAZGQL010000012 DAZGQL010000000
VERSION     DAZGQL010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 69192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 69192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..69192
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.012"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(7..83)
                     /locus_tag="ACHKIR_000938"
     tRNA            complement(7..83)
                     /locus_tag="ACHKIR_000938"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(47..49),aa:Arg,seq:acg)
     gene            complement(282..358)
                     /locus_tag="ACHKIR_000939"
     tRNA            complement(282..358)
                     /locus_tag="ACHKIR_000939"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(322..324),aa:Arg,seq:acg)
     gene            complement(362..454)
                     /locus_tag="ACHKIR_000940"
     tRNA            complement(362..454)
                     /locus_tag="ACHKIR_000940"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(418..420),aa:Ser,seq:gct)
     gene            complement(770..955)
                     /gene="csrA"
                     /locus_tag="ACHKIR_000941"
     CDS             complement(770..955)
                     /gene="csrA"
                     /locus_tag="ACHKIR_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HFO9988717.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(1190..3820)
                     /gene="alaS"
                     /locus_tag="ACHKIR_000942"
     CDS             complement(1190..3820)
                     /gene="alaS"
                     /locus_tag="ACHKIR_000942"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFO9988718.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVNSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAIATVHADSDRLNEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3948..4448)
                     /gene="recX"
                     /locus_tag="ACHKIR_000943"
     CDS             complement(3948..4448)
                     /gene="recX"
                     /locus_tag="ACHKIR_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFO9988719.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4517..5578)
                     /gene="recA"
                     /locus_tag="ACHKIR_000944"
     CDS             complement(4517..5578)
                     /gene="recA"
                     /locus_tag="ACHKIR_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFO9988720.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5658..6155)
                     /gene="pncC"
                     /locus_tag="ACHKIR_000945"
     CDS             complement(5658..6155)
                     /gene="pncC"
                     /locus_tag="ACHKIR_000945"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HFO9988721.1"
                     /translation="MTDSELMQLSEQVGQSLKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMVIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(6300..7385)
                     /gene="mltB"
                     /locus_tag="ACHKIR_000946"
     CDS             complement(6300..7385)
                     /gene="mltB"
                     /locus_tag="ACHKIR_000946"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HFO9988722.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            7641..8204
                     /gene="srlA"
                     /locus_tag="ACHKIR_000947"
                     /gene_synonym="gutA"
     CDS             7641..8204
                     /gene="srlA"
                     /locus_tag="ACHKIR_000947"
                     /gene_synonym="gutA"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFO9988723.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            8201..9160
                     /gene="srlE"
                     /locus_tag="ACHKIR_000948"
                     /gene_synonym="gutE"
     CDS             8201..9160
                     /gene="srlE"
                     /locus_tag="ACHKIR_000948"
                     /gene_synonym="gutE"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFO9988724.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            9171..9542
                     /gene="srlB"
                     /locus_tag="ACHKIR_000949"
                     /gene_synonym="gutB"
     CDS             9171..9542
                     /gene="srlB"
                     /locus_tag="ACHKIR_000949"
                     /gene_synonym="gutB"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFO9988725.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            9546..10325
                     /gene="srlD"
                     /locus_tag="ACHKIR_000950"
     CDS             9546..10325
                     /gene="srlD"
                     /locus_tag="ACHKIR_000950"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HFO9988726.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            10431..10790
                     /gene="gutM"
                     /locus_tag="ACHKIR_000951"
     CDS             10431..10790
                     /gene="gutM"
                     /locus_tag="ACHKIR_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFO9988727.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIVDTLFMKGLTVFARPQKIPAITGMYAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            10857..11630
                     /gene="srlR"
                     /locus_tag="ACHKIR_000952"
                     /gene_synonym="gutR"
     CDS             10857..11630
                     /gene="srlR"
                     /locus_tag="ACHKIR_000952"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HFO9988728.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEKKGIDVIITGESNE"
     gene            11623..12588
                     /gene="gutQ"
                     /locus_tag="ACHKIR_000953"
     CDS             11623..12588
                     /gene="gutQ"
                     /locus_tag="ACHKIR_000953"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HFO9988729.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDDQRLVKGVFTDGDLRRWLVGGGALTTPVNEAMTVGGTTL
                     QSQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(12585..14099)
                     /gene="norR"
                     /locus_tag="ACHKIR_000954"
     CDS             complement(12585..14099)
                     /gene="norR"
                     /locus_tag="ACHKIR_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HFO9988730.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDAAPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYSFPGNVRELEHAIHRAVVLSRATRSGDEVILEAQHFAFPEVTLPPPEAAAVP
                     IVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            14286..15725
                     /gene="norV"
                     /locus_tag="ACHKIR_000955"
     CDS             14286..15725
                     /gene="norV"
                     /locus_tag="ACHKIR_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016966 - nitric oxide reductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HFO9988731.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLTDIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            15722..16855
                     /gene="norW"
                     /locus_tag="ACHKIR_000956"
     CDS             15722..16855
                     /gene="norW"
                     /locus_tag="ACHKIR_000956"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016731 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HFO9988732.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDACIPLTLIAADSMDEYNKP
                     DLSHVISQRQRADDLTRQTAGEFAEQFNLHLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRNARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILAALMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDRQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPV"
     gene            complement(17079..19331)
                     /gene="hypF"
                     /locus_tag="ACHKIR_000957"
     CDS             complement(17079..19331)
                     /gene="hypF"
                     /locus_tag="ACHKIR_000957"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HFO9988733.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSAGGVMNTQI
                     VPDAATCPACLAEMNTPGERRWRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVAEGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDAHPGYVSSQWAREMNLPTQTVLHHHAHAAACLAEHHWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECQHLGGLPAVALAGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAGLGCAPATLSYEGEAACALEA
                     LAASCDGVTHPVTMPLVDNQLDLATFWQQWLSWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(19484..20011)
                     /gene="hydN"
                     /locus_tag="ACHKIR_000958"
     CDS             complement(19484..20011)
                     /gene="hydN"
                     /locus_tag="ACHKIR_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HFO9988734.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(20160..21170)
                     /gene="ascG"
                     /locus_tag="ACHKIR_000959"
     CDS             complement(20160..21170)
                     /gene="ascG"
                     /locus_tag="ACHKIR_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HFO9988735.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSVERLAGYKDALAQHGIALNEKLIA
                     NGKWTPASGAEGVEMLLERGAKFSALVASNDDMAIGAMKALHERGVAVPEQVSVIGFD
                     DIAIAPYTVPALSSVKIPVTEMIQEIIGRLIFMLDGGAYTPPKTFSGKLIRRDSLIAL
                     SR"
     gene            21430..22887
                     /gene="ascF"
                     /locus_tag="ACHKIR_000960"
     CDS             21430..22887
                     /gene="ascF"
                     /locus_tag="ACHKIR_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HFO9988736.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRFVIKDDQLIDS
                     PTLKTISGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEATAEARKHQSAQPTVAKEVSLN"
     gene            22896..24320
                     /gene="ascB"
                     /locus_tag="ACHKIR_000961"
     CDS             22896..24320
                     /gene="ascB"
                     /locus_tag="ACHKIR_000961"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HFO9988737.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAYREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     ELTPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLIASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPA
                     YSARVFREKGVTIDKAPGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LARTRKKSFWWYKKVIASNGEDLE"
     gene            complement(24478..24948)
                     /gene="hycI"
                     /locus_tag="ACHKIR_000962"
     CDS             complement(24478..24948)
                     /gene="hycI"
                     /locus_tag="ACHKIR_000962"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HFO9988738.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            complement(24941..25351)
                     /gene="hycH"
                     /locus_tag="ACHKIR_000963"
     CDS             complement(24941..25351)
                     /gene="hycH"
                     /locus_tag="ACHKIR_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HFO9988739.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDDYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQKEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(25348..26115)
                     /gene="hycG"
                     /locus_tag="ACHKIR_000964"
     CDS             complement(25348..26115)
                     /gene="hycG"
                     /locus_tag="ACHKIR_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HFO9988740.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDYLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            complement(26115..26657)
                     /gene="hycF"
                     /locus_tag="ACHKIR_000965"
     CDS             complement(26115..26657)
                     /gene="hycF"
                     /locus_tag="ACHKIR_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HFO9988741.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(26667..28376)
                     /gene="hycE"
                     /locus_tag="ACHKIR_000966"
     CDS             complement(26667..28376)
                     /gene="hycE"
                     /locus_tag="ACHKIR_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HFO9988742.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(28394..29317)
                     /gene="hycD"
                     /locus_tag="ACHKIR_000967"
     CDS             complement(28394..29317)
                     /gene="hycD"
                     /locus_tag="ACHKIR_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HFO9988743.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(29320..31146)
                     /gene="hycC"
                     /locus_tag="ACHKIR_000968"
     CDS             complement(29320..31146)
                     /gene="hycC"
                     /locus_tag="ACHKIR_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HFO9988744.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEIMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLAIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGILTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(31143..31754)
                     /gene="hycB"
                     /locus_tag="ACHKIR_000969"
     CDS             complement(31143..31754)
                     /gene="hycB"
                     /locus_tag="ACHKIR_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HFO9988745.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(31879..32340)
                     /gene="hycA"
                     /locus_tag="ACHKIR_000970"
     CDS             complement(31879..32340)
                     /gene="hycA"
                     /locus_tag="ACHKIR_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HFO9988746.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            32552..32902
                     /gene="hypA"
                     /locus_tag="ACHKIR_000971"
     CDS             32552..32902
                     /gene="hypA"
                     /locus_tag="ACHKIR_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFO9988747.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            32906..33778
                     /gene="hypB"
                     /locus_tag="ACHKIR_000972"
     CDS             32906..33778
                     /gene="hypB"
                     /locus_tag="ACHKIR_000972"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HFO9988748.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            33769..34041
                     /gene="hypC"
                     /locus_tag="ACHKIR_000973"
     CDS             33769..34041
                     /gene="hypC"
                     /locus_tag="ACHKIR_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HFO9988749.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            34041..35162
                     /gene="hypD"
                     /locus_tag="ACHKIR_000974"
     CDS             34041..35162
                     /gene="hypD"
                     /locus_tag="ACHKIR_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HFO9988750.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAETHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            35159..36169
                     /gene="hypE"
                     /locus_tag="ACHKIR_000975"
     CDS             35159..36169
                     /gene="hypE"
                     /locus_tag="ACHKIR_000975"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HFO9988751.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARAAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIELSEAALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            36243..38321
                     /gene="flhA"
                     /locus_tag="ACHKIR_000976"
     CDS             36243..38321
                     /gene="flhA"
                     /locus_tag="ACHKIR_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HFO9988752.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLATGGLYPKFGHYCLMPLAAEGHIFGGCEFIRHDDRPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     HERDDLAPYERMLFNTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLSLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRILSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            38366..38794
                     /locus_tag="ACHKIR_000977"
     CDS             38366..38794
                     /locus_tag="ACHKIR_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012134844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HFO9988753.1"
                     /translation="MAVSARNQLTGTVSTVAMGAVNDEVELTLAGGAKLVAIVTHSSQ
                     QALGLAKGKEAIALIKAPWVTLATEDCGLKFSARNQFAGSVSTITEGAVNATVHIKTD
                     AGFEIVAVVTNESQDEMKLTTGSRVIALIKASAILIATKA"
     gene            complement(38833..39177)
                     /gene="ygbA"
                     /locus_tag="ACHKIR_000978"
     CDS             complement(38833..39177)
                     /gene="ygbA"
                     /locus_tag="ACHKIR_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HFO9988754.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFAYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKK"
     gene            39463..42024
                     /gene="mutS"
                     /locus_tag="ACHKIR_000979"
     CDS             39463..42024
                     /gene="mutS"
                     /locus_tag="ACHKIR_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFO9988755.1"
                     /translation="MRTIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMERQQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLEGVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIATGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            42130..42786
                     /gene="pphB"
                     /locus_tag="ACHKIR_000980"
     CDS             42130..42786
                     /gene="pphB"
                     /locus_tag="ACHKIR_000980"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HFO9988756.1"
                     /translation="MPSTRYQKINAHHYHHIWAVGDIHGDYQLLQSRLHQLSFCPETD
                     LLISVGDNIDRGPESLNVLRLLNQPWFISVKGNHEAMALEAFETGDGNMWLASGGDWF
                     FDLNDSEQQEAIDLLLKFHHLPHIIEITNDTIKYVIAHADYPGKEYQFGKEIAESELL
                     WPVDRVQKSLNSELQKINGADYFIFGHMMFDNIQTFANQIYIDTGSPKSGRLSFYKIK
                     "
     gene            complement(42837..43604)
                     /gene="ygbI"
                     /locus_tag="ACHKIR_000981"
     CDS             complement(42837..43604)
                     /gene="ygbI"
                     /locus_tag="ACHKIR_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor YgbI"
                     /protein_id="HFO9988757.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRMNVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGRCIYLDAGT
                     TTLAIAQHLTHMEPLTVVTNDFVIADYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKLDLSLLVAKNE"
     gene            43800..44708
                     /gene="ltnD"
                     /locus_tag="ACHKIR_000982"
     CDS             43800..44708
                     /gene="ltnD"
                     /locus_tag="ACHKIR_000982"
                     /EC_number="1.1.1.411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonate dehydrogenase"
                     /protein_id="HFO9988758.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            44705..45967
                     /gene="otnK"
                     /locus_tag="ACHKIR_000983"
     CDS             44705..45967
                     /gene="otnK"
                     /locus_tag="ACHKIR_000983"
                     /EC_number="2.7.1.217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate kinase"
                     /protein_id="HFO9988759.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSSAKGNIGPVTDAL
                     MDALDTPFTVFSPALPVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVEA
                     QSTGRCGVVSAHVFEQGVDAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDTPL
                     VTGGSGLAIGLARQWAQENGNQAREAGRPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVARCLSTETLAAYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVTRFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            45964..46602
                     /gene="ygbL"
                     /locus_tag="ACHKIR_000984"
     CDS             45964..46602
                     /gene="ygbL"
                     /locus_tag="ACHKIR_000984"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HFO9988760.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVTADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLEGLDSNNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            46607..47383
                     /gene="ygbM"
                     /locus_tag="ACHKIR_000985"
     CDS             46607..47383
                     /gene="ygbM"
                     /locus_tag="ACHKIR_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HFO9988761.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYDYSPQQ
                     IQQQLAQNHLTLALFNTAPGDINAGEWGLSALPGREHEARADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADHFAPHGKRILVEALSPGVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGCEYKPRGLTEEGLGWFDAWR"
     gene            47472..48836
                     /gene="ygbN"
                     /locus_tag="ACHKIR_000986"
     CDS             47472..48836
                     /gene="ygbN"
                     /locus_tag="ACHKIR_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HFO9988762.1"
                     /translation="MSTITLLCIALTGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVGFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVAAAGLLHADIGWLTIIGIAISIPVGIVGYFAAKIINKRQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMAGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCI
                     WAVI"
     gene            complement(48874..49110)
                     /locus_tag="ACHKIR_000987"
     CDS             complement(48874..49110)
                     /locus_tag="ACHKIR_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit D"
                     /protein_id="HFO9988763.1"
                     /translation="MICPRCADEQIEVMAKSPVKDVWMVYQCQHCLYTWRDTEPLRRT
                     SREHYPEAFRMTQKDIDDAPMVPSIPPLLAEGKR"
     gene            complement(49121..50548)
                     /locus_tag="ACHKIR_000988"
     CDS             complement(49121..50548)
                     /locus_tag="ACHKIR_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit C"
                     /protein_id="HFO9988764.1"
                     /translation="MAFDDLRSFLQALDDHGQLLKISEEVNAEPDLAAAANATGRIGD
                     GAPALWFDNIRGFIDARVAMNTIGSWQNHAISLGLPPNTPVKKQIDEFIRRWDNFPIA
                     PERRANPAWAQNTVDGDEINLFNILPLFRLNDGDGGFYLDKACVVSRDPLDPDNFGKQ
                     NVGIYRMEVKGKRKLGLQPVPMHDIALHLHKAEERGEDLPIAITLGNDPIITLMGATP
                     LKYDQSEYEMAGALRESPYPIATAPLTGFDVPWGSEVILEGVIESRKREIEGPFGEFT
                     GHYSGGRNMTVVRIDKVSYRTKPIFESLYLGMPWTEIDYLMGPATCVPLYQQLKAEFP
                     EVQAVNAMYTHGLLAIISTKKRYGGFARAVGLRAMTTPHGLGYVKMVIMVDEDVDPFN
                     LPQVMWALSSKVNPAGDLVQLPNMSVLELDPGSSPAGITDKLIIDATTPVAPDNRGHY
                     SQPVVDLPETKAWAEKLTAMLAARQ"
     gene            complement(50548..51141)
                     /locus_tag="ACHKIR_000989"
     CDS             complement(50548..51141)
                     /locus_tag="ACHKIR_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit B"
                     /protein_id="HFO9988765.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAATISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            51288..51695
                     /locus_tag="ACHKIR_000990"
     CDS             51288..51695
                     /locus_tag="ACHKIR_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001208086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFO9988766.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDAADKRRRFVWLTAEGEKVLAAA
                     IPIGDSVDEEFLGRLSAEEQELFMQLVRKMMSK"
     gene            complement(51816..52808)
                     /gene="rpoS"
                     /locus_tag="ACHKIR_000991"
     CDS             complement(51816..52808)
                     /gene="rpoS"
                     /locus_tag="ACHKIR_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HFO9988767.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(52871..53965)
                     /gene="nlpD"
                     /locus_tag="ACHKIR_000992"
     CDS             complement(52871..53965)
                     /gene="nlpD"
                     /locus_tag="ACHKIR_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HFO9988768.1"
                     /translation="MSLVSLWLAGCSDTSNPPAPVSSVNGNAPANTNSGMLITPPPKM
                     GTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENGRIVYNRQYGNIPKGS
                     YSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITG
                     GNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSANKMLPNNKPTATTVT
                     APVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASEGGNKGIDIAGSKGQA
                     IIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATM
                     GSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(54150..54776)
                     /gene="pcm"
                     /locus_tag="ACHKIR_000993"
     CDS             complement(54150..54776)
                     /gene="pcm"
                     /locus_tag="ACHKIR_000993"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HFO9988769.1"
                     /translation="MVSRRVQALLDQLRAQGIQDELVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(54770..55531)
                     /gene="surE"
                     /locus_tag="ACHKIR_000994"
     CDS             complement(54770..55531)
                     /gene="surE"
                     /locus_tag="ACHKIR_000994"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HFO9988770.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(55512..56561)
                     /gene="truD"
                     /locus_tag="ACHKIR_000995"
     CDS             complement(55512..56561)
                     /gene="truD"
                     /locus_tag="ACHKIR_000995"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HFO9988771.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAEETELQTLLVREKVEAARRAMLLYPQKLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(56558..57037)
                     /gene="ispF"
                     /locus_tag="ACHKIR_000996"
     CDS             complement(56558..57037)
                     /gene="ispF"
                     /locus_tag="ACHKIR_000996"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HFO9988772.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYALGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(57037..57747)
                     /gene="ispD"
                     /locus_tag="ACHKIR_000997"
     CDS             complement(57037..57747)
                     /gene="ispD"
                     /locus_tag="ACHKIR_000997"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFO9988773.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVYA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPRITVVDGGEERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(57766..58077)
                     /gene="ftsB"
                     /locus_tag="ACHKIR_000998"
     CDS             complement(57766..58077)
                     /gene="ftsB"
                     /locus_tag="ACHKIR_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HFO9988774.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNNDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(58270..58593)
                     /gene="ygbE"
                     /locus_tag="ACHKIR_000999"
     CDS             complement(58270..58593)
                     /gene="ygbE"
                     /locus_tag="ACHKIR_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HFO9988775.1"
                     /translation="MRNSHNITLTNNDSLTEVEDTTWSLPGAVVGFLSWLCALAIPML
                     IYGSNTLLFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLATVGIMFGALF
                     MWLLG"
     gene            complement(58643..59248)
                     /gene="cysC"
                     /locus_tag="ACHKIR_001000"
     CDS             complement(58643..59248)
                     /gene="cysC"
                     /locus_tag="ACHKIR_001000"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HFO9988776.1"
                     /translation="MALHDENVVWHSHPVTPQQREQHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(59248..60675)
                     /gene="cysN"
                     /locus_tag="ACHKIR_001001"
     CDS             complement(59248..60675)
                     /gene="cysN"
                     /locus_tag="ACHKIR_001001"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HFO9988777.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(60677..61585)
                     /gene="cysD"
                     /locus_tag="ACHKIR_001002"
     CDS             complement(60677..61585)
                     /gene="cysD"
                     /locus_tag="ACHKIR_001002"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HFO9988778.1"
                     /translation="MDQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            61837..62874
                     /gene="iap"
                     /locus_tag="ACHKIR_001003"
     CDS             61837..62874
                     /gene="iap"
                     /locus_tag="ACHKIR_001003"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HFO9988779.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKSWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGIDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     repeat_region   62895..63350
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=63017..63043
                     /rpt_unit_seq="cggtttatccccgctggcgcggggaac"
     gene            complement(63456..63740)
                     /gene="cas2e"
                     /locus_tag="ACHKIR_001004"
     CDS             complement(63456..63740)
                     /gene="cas2e"
                     /locus_tag="ACHKIR_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2e"
                     /protein_id="HFO9988780.1"
                     /translation="MSMLVVVTENVPSRLRGRLAIWLLEVRAGVYVGDVSARVREMIW
                     EQISGLAEEGNVVMAWATNTESGFEFQTFGVNRRTPVDLDGLRLVSFLPV"
     gene            complement(63742..64659)
                     /gene="cas1e"
                     /locus_tag="ACHKIR_001005"
     CDS             complement(63742..64659)
                     /gene="cas1e"
                     /locus_tag="ACHKIR_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1e"
                     /protein_id="HFO9988781.1"
                     /translation="MTWLPLNPIPLKDRVSMIFLQYGQIDVIDGAFVLIDKTGIRTHI
                     PVGSVACIMLEPGTRVSHAAVRLAAQVGTLLVWVGEAGVRVYASGQPGGARSDKLLYQ
                     AKLALDEDLRLKVVRKMFELRFGEPAPARRSVEQLRGIEGSRVRATYVLLAKQYGVKW
                     NGRRYDPKDWEKGDTVNQCISAATSCLYGVTEAAILAAGYAPAIGFVHTGKPLSFVYD
                     IADIIKFDTVVPKAFEIARRIPGEPDREVRLACRDIFRSSKTLVKLIPLIEDVLAAGE
                     IQPPAPPEDAQPVAIPLPVSLGDAGHRSS"
     gene            complement(64675..65274)
                     /gene="cas6e"
                     /locus_tag="ACHKIR_001006"
     CDS             complement(64675..65274)
                     /gene="cas6e"
                     /locus_tag="ACHKIR_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HFO9988782.1"
                     /translation="MYLSKVIIARAWSRDLYQLHQGLWHLFPNRPDAARDFLFHVEKR
                     NTPEGCHVLLQSAQMPVSTAVATVIKTKQVEFQLQVGVPLYFRLRANPIKTILDNQKR
                     LDSKGNIKRCRVPLIKEAEQIAWLQRKLGNAARVEDVHPISERPQYFSGDGKSGKIQT
                     VCFEGVITINDAPALIDLVQQGIGPAKSMGCGLLSLAPL"
     gene            complement(65261..65935)
                     /gene="cas5e"
                     /locus_tag="ACHKIR_001007"
     CDS             complement(65261..65935)
                     /gene="cas5e"
                     /locus_tag="ACHKIR_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HFO9988783.1"
                     /translation="MRSYLILRLAGPMQAWGQPTFEGTRPTGRFPTRSGLLGLLGACL
                     GIQRDDTSSLQALSESVQFAVRCDELILDDRRVSVTGLRDYHTVLGAREDYRGLKSHE
                     TIQTWREYLCDASFTVAIWLTPQATMVMSELEKAVLKPRYTPYLGRRSCPLTQPLFLG
                     TCQASDPQKALLNYEPVGGDIYSEESVDGHHLKFTVRDEPMITLPRQFASREWYVIKG
                     GMDVSQ"
     gene            complement(65938..67029)
                     /gene="cas7e"
                     /locus_tag="ACHKIR_001008"
     CDS             complement(65938..67029)
                     /gene="cas7e"
                     /locus_tag="ACHKIR_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HFO9988784.1"
                     /translation="MSNFINIHVLISHSPSCLNRDDMNMQKDAIFGGKRRVRISSQSL
                     KHAMRKSDYYAQNIGESSLRTIHLAQLRDVLRQKLGERFDQKIIDKTLALLSGKLVDE
                     AEKISADAVTPWVVGEIAWFCEQVAKAEADNLDDKKLLKVLKEDIAAIRVNLQQGVDI
                     ALSGRMATSGMMSELGKVDGAMSIAHAITTHQVDSDIDWFTAVDDLQEQGSAHLGTQE
                     FSSGVFYRYANINLAQLQENLGGASREQALEIATHVVHMLATEVPGAKQRTYAAFNPA
                     DMVMVNFSDMPLSMANAFEKAVKAKDGFLQPSIQAFNQYWDRVANGYGLNGAAAQFSL
                     SDVDPITAQVKQMPTLEQLKSWVRNNGEA"
     gene            complement(67042..67524)
                     /gene="casB"
                     /locus_tag="ACHKIR_001009"
                     /gene_synonym="cse2"
     CDS             complement(67042..67524)
                     /gene="casB"
                     /locus_tag="ACHKIR_001009"
                     /gene_synonym="cse2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HFO9988785.1"
                     /translation="MADEIDAMALYRAWQQLDNGACAQIRRVSEPDELRDIPAFYRLV
                     QSFGWENPRHQQALLRMVFCLSAGKNVIRHQDKKTEQTTGISLGRALANSGRINERRI
                     FQLIRADRTADMVQLRRLLTHAEPVLDWPLMARMLTWWGKRERQQLLEDFVLTTNKNA
                     "
     gene            complement(67517..69025)
                     /gene="casA"
                     /locus_tag="ACHKIR_001010"
     CDS             complement(67517..69025)
                     /gene="casA"
                     /locus_tag="ACHKIR_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HFO9988786.1"
                     /translation="MNLLIDNWIPVRPRNGGKVQIINLQSLYCSRDQWRLSLPRDDME
                     LATLALLVCIGQIIAPAKDDVEFRHRIMNPLTEDEFQQLIAPWIDMFYLNHAEHPFMQ
                     TKGVKANDVTPMEKLLAGVSGATNCAFVNQPGQGEALCGGCTAIALFNQANQAPGFGG
                     GFKSGLRGGTPITTFVCGIDLRSTVLLNVLTIPRLQKQFPNELHTENQPTWVKPVKPN
                     ESVPASSIGFVRGLFWQPAHIELCDPIGIGKCSCCGQESNLRYTGFLKEKFTFTVNGL
                     WPHPHSPCLVTVKKGEVEEKFLAFTTSAPSWTQISRVVVDKIIQNENGNRVAAVVNQF
                     RNIAPQSPLELIMGGYRNNQASILERRHDVLMFNQGWQQYGNVINEIVTVGLGYKTAL
                     RKALYTFAEGFKNKDFKGAGVSVHETAERHFYRQSELLIPDVLANINFSQADEVIADL
                     RDKLHQLCEMLFNQSVAPYAHHPKLISTLALARATLYKHLRELKPQGGPSNG"
BASE COUNT        16503 a        18774 c        17661 g        16254 t
ORIGIN      
        1 aagagatggt gcatccggga ggattcgaac ctccgaccgc tcggttcgta gccgagtact
       61 ctatccagct gagctacgga tgcatcggga aacttacttt actgcagatt ttttgatacc
      121 gctactaaag ccgtatcaag taagagatgg tgcatccggg aggattactc gccttgcggc
      181 tcgcccttcg ggccgttgct aaagcaacgt tatcctccct ggtgcctgcg gttattaccg
      241 ccacctcttc cataccttca tcgctgaagg tacgtagaat atggtgcatc cgggaggatt
      301 cgaacctccg accgctcggt tcgtagccga gtactctatc cagctgagct acggatgcaa
      361 atggcggtga ggcggggatt cgaaccccgg atgcagcttt tgaccgcata ctcccttagc
      421 aggggagcgc cttcagcctc tcggccacct caccacacgc ctcttacgag tgcttcgaag
      481 aacttgttta tcgctcatcg tcgctgcgtg gcgcacatat tactttctga gacttataag
      541 tcaaacaatt tttcccacac ttttatcgtt tgcacacttc acgctcaatt agtctgtaaa
      601 aacgtcaaaa agagtgtttt atcaacagaa aaacggaggt ctgtcagata gcagtaatgc
      661 aaaaaaatgg agacttaagt tgaatgaacg ggagtaaagc gaaaagacaa tggagtgaag
      721 aagaaatttt gagggtgcgt ctcaccgata aagatgagac gcggaaagat tagtaactgg
      781 actgctggga tttttcagcc tggatacgct ggtagatctc ttcacggtga acagaaactt
      841 ccttcggggc atttacgcca atacgtacct ggttgccctt tacccctaaa actgtcacgg
      901 tgacctcatc cccaatcatg agggtctcac caactcgacg agtcagaatc agcattcttt
      961 gctccttgaa agattaaaag agtcgggtct ctctgtatcc cggcattatc catcatataa
     1021 cgccaaaaag taagcaatga caaacacatt acatctaagc agtcatggca ttacattctg
     1081 ttaaacctaa gtttagccga tatacacaac ttcaacctga ctttatcgtt gtcgatagcg
     1141 ttaatgcgaa tgccgtgaag cgagtccacg gcattgcctg acgcttatat tattgcaatt
     1201 tcgcgctgac ccagcctttc acactggcta acgctgcagg taaggccgca gcatccgtac
     1261 caccggcttg cgccatgtca ggacgtccac cacccttgcc gcccacctgc tgagcgacca
     1321 taccaatcag ttcccctgct ttcacacgat ctgtgacgtc cttagatacg cctgcaatca
     1381 gagaaacctt accttcggct accgttgcca gcacgataat tgtcgacccc agctgatttt
     1441 ttaaatcgtc aaccatggta cgcaacattt tcggctcaac accgctaagc tcgctaacca
     1501 acagcttaac accattaaca tcaattgcct tactggaaag atttgcgctc tcctgtgcgg
     1561 cagcttgttc tttaagctgt tgtaactctt tttccagctg acgcgtacgt tccagtactg
     1621 agcgcacttt atcagccaga ttattgctat cgcctttcag cagatgcgcg acttcgttta
     1681 agcgatcgct gtctgcatga acggtggcga tagcgccttc tccggttacc gcttcgatac
     1741 gacgaacgcc tgcagcagta cccgattcag agatgatgcg gaacagacca atatcaccag
     1801 tgcgactggc gtgagtaccg ccacacagct cggtggagaa atcgcccatg ctcagcacac
     1861 gtacacgttc atcatacttc tcgccgaaca gcgccatcgc acctttcgct ttcgccgctt
     1921 cgagatccat gatgttggtt tcaatcggca agttacggcg aatctgtgcg ttcaccaggt
     1981 cttcgaccgc acgaatctct tctggtttca tcgcttcgtt gtgtgagaag tcgaagcgca
     2041 gcactttgtc gttaaccagt gaacctttct gcgatacatg agtacccaga acctggcgca
     2101 gcgcagcgtg catcaggtgc gttgcggagt gattcagacg aatacgggcg cgacgagctt
     2161 catcaacatc agcctgcacc gcgtcgccca ctttcagaga acccgcagca agtttaccga
     2221 tgtgaccaat cgcctggccg tatttctgcg tatcttccac cgcaaaggag aagttagcgc
     2281 ctttcagttc gcctttatca ccaacctgac cgccggattc cgcatagaat ggcgtttgat
     2341 ccagcacgac cacagcttcc tggcctgcat tgatggcatc aaccgcttta ccatcaacaa
     2401 acagcgcggt cactttgccg ttcagttcca gatggtcata gcctttaaat tcagatgcac
     2461 tgttaacacg gatcattgcg ttgtaatcgg caccaaagcc gctggcttcg cgcgcacgac
     2521 gacgctgctc ttccattgct gcttcaaatc cagcttcgtc aactttgatg ttgcgctcac
     2581 gacaaacatc agccgtcagg tcaaccggga agccataggt gtcgtacaga cggaaagccg
     2641 tttcaccatc cagcgtatca ccagaaagtt ttgccagctc ttcgtccagc agcgccagac
     2701 cgcgctccag agtacgggca aactgctctt cttcagtctt cagcacctgc tcaacctgcg
     2761 cctgctggcg tttcaggtct tcacccgcag agcccattac gtcgatcagc ggaccaacca
     2821 gtttgtagaa gaaggtttct ttcgcgccca gcatattgcc gtgacgcact gcgcgacgaa
     2881 tgatacgacg cagtacataa ccacggtttt cgttggacgg cattacgcca tccgcgatca
     2941 ggaacgcaca agaacgaatg tggtcagcga ttacgcgcag cgatttgttg ctcaaatcgg
     3001 tcgcgccagt gactttcgct accgcctgga tcagcgtacg gaacaggtcg atgtcatagt
     3061 tagagttaac gtgttgcagc accgcagcaa tacgctccag acccataccg gtatctacag
     3121 acggcttcgg cagcggttcc atcgtgccat ctgcctggcg gttgaactgc atgaagacga
     3181 tgttccagat ctcaatgtag cggtcgccgt cttcttccgg acttcccgga gggccacccc
     3241 aaatgtggtc gccgtgatcg tagaagattt cggtgcacgg gccgcacgga ccagtgtcac
     3301 ccatctgcca gaagttgtca gatgcgtatg gcgcaccttt gttatcgccg atgcgaataa
     3361 tacgttcgcg cgggatcccg acttcttttt cccagatttc gtaggcttcg tcgtcgcttt
     3421 catagacggt aacccacaga cgctctttcg gcagggcaaa ccatttttcg ctggtcagca
     3481 gttcccatgc aaactgaatg gcatcgtgtt tgaaatagtc gccgaagctg aagttgccca
     3541 gcatttcgaa gaaggtatgg tgacgcgcgg tgtaaccgac gttttccagg tcgttgtgtt
     3601 taccacccgc acgcacgcag cgttgggaag tggtagcgcg ggaataatta cgcttgtcga
     3661 gcccaaggaa cacatccttg aactggttca tcccggcgtt ggtaaacaac aaagttgggt
     3721 cgttatgggg taccagggag ctgctggcaa ctacctgatg tcccttacta tggaaaaagt
     3781 cgagaaacgc ctgacggatc tcagcggtgc tcttgctcat aattatcctg aaatcaagct
     3841 aacgaaatat cgccaccagc tacagcgtgt tttaaccgcc gggctggtaa ctgaaaagtg
     3901 ggaataagat aagttttctt gactgggaag taaaatcccg tatgcgttca gtcggcaaaa
     3961 tttcgccaaa tatcctggat atcttccatc agatagccac gatagagcag aaaacgctgg
     4021 atcttaactt tttctgaaaa gacagttggc aaaggttcgc catattttcg cgtcgcctga
     4081 tcgcgcgcca gtgcgcacca gtcgatgtca cattcacgca tcgctttttc tgtcgcttcg
     4141 cgggaaatac ctttctgatt cagttcctga cgaatacgcg caggtccata acctttgcgg
     4201 ctacggctgg cgataaagcg cgcaacaaat cggctgtcat cgagatagcc atgttcatgg
     4261 caccaggcga taacgcgctc gtaatcttct gccgtggcat caatctcttc cgggccattt
     4321 ttgcccatga tcggtgccgc gagtttacgt cgcaattctt gctcactgtg atcgcgcacc
     4381 gccagaatgc gtaccgcacg atccaacagg cgagcatatg ccgggcgacg ggacgttgat
     4441 tctgtcatgg catatcctta caacttaaaa aagcaaaagg gccgcagatg cgacccttgt
     4501 gtatcaaaca agacgattaa aaatcttcgt tagtttctgc tacgccttcg ctgtcatcta
     4561 cagagaaatc cggcgttgag ttcgggttgc tcagcagcaa ctcacgtact ttcttctcga
     4621 tctctttcgc ggtttccggg ttatctttca gccaggcagt tgcattcgct ttaccttgac
     4681 cgatcttctc acctttgtag ctgtaccacg cgcctgcttt ctcgatcagc ttctctttca
     4741 cgcccaggtc aaccagttcg ccgtagaagt tgataccttc gccgtagagg atctggaatt
     4801 cagcctgttt aaacggcgca gcgattttgt tcttcaccac tttcacacgg gtttcgctcc
     4861 ccaccacgtt ttcgccctct ttcaccgcgc cgatacgacg gatgtcgaga cgaacagagg
     4921 cgtagaattt cagcgcgtta ccaccggtag tggtttccgg gttaccgaac attacaccaa
     4981 ttttcatacg gatctggttg atgaagatca gcagcgtgtt ggactgcttc aggttacccg
     5041 ccagcttacg catcgcctgg ctcatcatac gtgccgcaag gcccatgtga gagtcgccga
     5101 tttcgccttc gatttccgct ttcggcgtca gtgccgccac ggagtcaacg acgataacgt
     5161 ctactgcgcc agaacgcgcc agggcgtcac agatttccag tgcctgctcg ccggtatccg
     5221 gctgggagca cagcaggttg tcgatatcaa cgcccagttt acgtgcgtag attgggtcca
     5281 gcgcgtgttc agcatcgata aacgcacagg ttttaccttc gcgctgcgct gcggcgatca
     5341 cctgcaacgt cagcgtggtt ttaccggaag attctggtcc gtagatttcg acgatacggc
     5401 ccatcggcag accacctgcc ccaagcgcga tatccagtga aagcgaaccg gtagagatgg
     5461 tttccacatc catggaacgg tcttcaccca ggcgcatgat ggagccttta ccaaattgtt
     5521 tctcaatctg gcccagtgct gccgccaacg ctttctgttt gttttcgtcg atagccattt
     5581 ttactcctgt catgccgggt aataccggat agtcaatatg ttctgttgaa gcaattatac
     5641 tgtatgctca tacagtatca agtgttttgt agaaattgtt gccacaaggt ctgcaatgca
     5701 tacgcagtag cctgacgacg caccgcatca cggtcaccgc tgaaacattc ccgccgggta
     5761 atgccttcac cacgggcagt ggcaaaagca aaccagacgg tgccgacagg tttctcttca
     5821 ctgccgccat ccggcccggc gataccacta atagacacgg cgtaatcagc acgagccgct
     5881 ttcagtgcgc ctatcaccat ttccaccacg acaggttcgc tcaccgcgcc atgctgtgcc
     5941 agcgtctctt cgcgtacacc gatcatttgc gcttttgctt cgttactgta ggtgacaaat
     6001 ccgcgttcaa accaggcgga actgccggca atatcggtaa tcactttcgc tacccaaccg
     6061 ccggtacaag actcagcagt tgttacggtt gcgccacggg ctttcagcga ctgcccaacc
     6121 tgttcgctta actgcatcag ttcactgtca gtcatcacaa cctctgtcag tcaaaaattt
     6181 atgccggaca agataacact ttcgtcagga agatggtgtt ggcgttgcaa ttttacgagg
     6241 aggattcaga aaaaagctga ttagccagag ggaagctcac gcccccctct tacaaatagt
     6301 tactgtacgc gcgccagcgc cacggcttgt cctaactgcc agaccgccat tgcgtaatgg
     6361 gtgctgtggt tataacgggt aatggtgtag aagttcggca gaccgtacca gtactggtag
     6421 ccggtgccaa catccagacg cagcaggctg gcttgttgat ggttgcccag cggctgctgt
     6481 ggcgttaaac ctgccgcggc aagctgcgaa atgctgtact tggttttgaa gccatttggc
     6541 aagcctggag cctgaccgtt tgccatcacc gcaacctgat cacctttcac ccagccgtgc
     6601 gctttgaaat agttcgccac actaccaatg gcatcaaccg gatcccacag gttgatatgc
     6661 ccgtcgccgc tgaaatctac cgcatattgt ttgtaagacg acggcataaa ctgtccgtag
     6721 cccatcgccc cggcaaagga acctttcaga ttgagcggat cgtcctgctc gtcgcgcgcc
     6781 atcagcagga aggtttccag ctcgccagaa aaatactccg cgcggcgtgg gtagttaaat
     6841 gacagcgttg ccagcgcatc gaggatgcga gttttcccca tcacgcgccc ccagcgggtt
     6901 tcaacgccga taatcccgac gataatttcc ggcggtacac catacacctg ccacgcgcga
     6961 ttcaacgcat cttcatactg attccagaaa accacaccgt tctgcacgtt gtccggcgta
     7021 ataaattttt tgcgataacg cagccatgcg ccgttcggac ctgatggcgg tttcaccgat
     7081 gtggttggtg cctggttatc catcagccgc agtaccgaat ccagacgttt cgcctgggag
     7141 agaatttcct gcaactgctg acgatcgaaa ccgtgtttgt tcaccatttt gtcgatgaac
     7201 tgctgggcat tcggattatt ggcgaagtca ccgcccatct gcatcacatt gtgctgcggc
     7261 tcaagcagga agccgccaga cggcgttccg gtggtcgttt cagtctcagt aggttttggc
     7321 ttgctgctac aggcggcaag caacacaaaa aggggaagca atgttacata acgacgcttg
     7381 aacatgaggg gtccatttaa cagattcaac caggggcaag tatggtaaag catcacgccc
     7441 cgcacaagga agcggtagtc actgcccgat acggacttta cataactcaa ctcattctcc
     7501 tcgctatcct tttattcaaa ctttcaaatt aaaatattta tctttcattt tgcgatcaaa
     7561 ataacacttt taaatctttc aatctgatta gattaggttg ccgtttggta ataaaacaat
     7621 aaatcctgaa ggagagaaca atgatagaaa ccattactca tggtgcagag tggtttatcg
     7681 ggctgttcca aaagggcgga gaggtgttta ccgggatggt gaccggcatt cttccgctgt
     7741 tgattagtct gctggttatc atgaacgcac tgattaattt tatcggtcag catcgtattg
     7801 aacgttttgc tcaacgttgc gccggtaacc ctgtttcccg ttacctgctg ttaccgtgca
     7861 ttggcacgtt tgtcttttgc aatccgatga ccttaagcct gggtcgcttt atgccggaaa
     7921 agtacaaacc cagctactac gcggccgcct cttatagctg ccactcaatg aatggcctct
     7981 tcccccatat caacccaggc gaactgtttg tttatcttgg cattgccagc ggtctgacaa
     8041 cactgaacct gccacttggc ccactggcgg tgagttatct gctggttggt ctggtcacca
     8101 acttcttccg cggctgggtg accgatctga ccaccgccat ttttgagaaa aagatgggca
     8161 ttcaacttga acaaaaagtt catctggcag gagcaacatc atgacgcgta ttcggatcga
     8221 aaaaggaacg ggtggctggg gcggcccgct tgagctggaa gccacgccgg gcaaaaaaat
     8281 cgtctatatc accgccggta cccggcctgc gattgttgac aaactggcac agcttactgg
     8341 ctggcaggct attgacggat ttaaagaagg tgaacccgcg gaggcggaaa ttggtgtcgc
     8401 ggtaatcgac tgtggcggca cattacgctg cggcatttat ccgaaacggc gtattcccac
     8461 cattaatatc cactcgacgg gcaagtccgg tccactagcg caatacattg tagaagatat
     8521 ttatgtctct ggcgtaaaag aagaaaacat cactgtagtg ggtgatgcga caccacaacc
     8581 ctcttccgtg ggccgtgact atgacaccag caagaaaatc accgaacaaa gcgatggttt
     8641 actggcgaag gtgggaatgg gtatgggttc tgccgttgcc gtgttgtttc aatctggtcg
     8701 tgacaccatc gacactgtat taaaaaccat tctgccgttt atggcattcg tttcggcgct
     8761 tatcggcatc attatggctt ccggtcttgg cgactggatt gcccacggtc ttgctccgct
     8821 ggcgagccat ccactgggtc tggtcatgct ggcgctcatc tgctccttcc cgctgctttc
     8881 acctttcctc ggcccaggcg cagttatcgc acaggttatc ggcgtattga ttggcgtgca
     8941 gattggtctc ggcaatattc cgccgcatct ggctttacct gcactgtttg ccatcaacgc
     9001 gcaggcggcc tgcgacttca tcccggtcgg tttgtcgctg gcggaagccc gtcaggacac
     9061 ggttcgcgtc ggtgtccctt ctgttctggt gagccgcttt ttaaccggtg cgccaactgt
     9121 actgatcgcc tggtttgtct ccggttttat ctatcaatag aggctgaaac atgaccgtta
     9181 tttatcagac caccatcacc cgtattggcg cgagtgccac tgacgccctc agcgaccaga
     9241 tgctcatcac ctttcgtgaa ggcgcgccag cggatctcga agagtattgc ttcattcatt
     9301 gccacggcga gttgaaaggt gcactccatc ccggtttgca attttcactc gggcagcatc
     9361 gctatccggt gaccgccgtt ggcagcgtgg cggaagacaa ccttcgcgaa ctgggtcatg
     9421 tcaccctgcg cttcgatggt ttaaacgaag cggaatttcc gggcactgtc catgtggcag
     9481 gccctgtacc cgacgatatc gcgccgggat cggttttgaa gtttgaatct gttaaggagt
     9541 aaaaaatgaa tcaggttgcc gttgtcatcg gtggtgggca aaccttaggc gcgttcctgt
     9601 gccacggtct ggctgccgag gggtatcgcg tcgcggttgt cgatattcag agcgacaaag
     9661 ccgcaaatgt ggcacaagaa attaacgccg aatatggtga aggtatggcg tacggttttg
     9721 gtgctgacgc cactagcgag caaagcgttc tggcgctctc tcgtggggta gatgaaatct
     9781 ttggtcgcgt ggatttgctg gtctacagcg ccggaatagc aaaagcagcc tttatcagcg
     9841 acttccagct cggcgatttt gaccgttcgc tacaggtgaa tctggtgggt tatttcctgt
     9901 gtgcccgtga attttcgcgt ttgatgatcc gcgacgggat tcaggggcgc attattcaga
     9961 tcaactcgaa atccggcaaa gtgggcagca aacacaactc tggctacagc gcagcgaaat
    10021 ttggtggcgt cgggctgact caatcactgg cgctggatct ggcggagtac ggcattacgg
    10081 tgcattcact gatgctcggt aatctgctga aatcgccgat gtttcagtcg ctattgccac
    10141 aatatgcgac caagctgggt atcaaaccgg atcaagtcga gcagtattac atcgacaaag
    10201 taccgctcaa acgcggctgc gattatcagg atgtgctgaa tatgctgctg ttctacgcca
    10261 gtcctaaggc gtcgtactgc actggtcagt cgatcaatgt caccggcggt caggtgatgt
    10321 tctgattaac agcggagatc cgttaaggat ctccgcgaga ctaataaaat gcctgatgcg
    10381 ctacgctcat caggcataca ggacttccgc cactacatta aggaaaagtt atggtatccg
    10441 cactcatcac cgtcgccgtt atcgcctggt gtgcgcaact ggccttaggc ggttggcaaa
    10501 tttctcgttt taaccgtgcc ttcgacacac tatgccagca agggcgggtt ggcgtgggcc
    10561 gttccagcgg gcgctttaaa ccgcgggtcg tggtcgccat cgccctggac gatcagcaac
    10621 gcatcgtcga caccttgttt atgaaaggac tgaccgtctt cgcccgacca caaaaaattc
    10681 ccgcaattac cggtatgtat gcgggtgatt tacagcccga tgtgatcttt ccccatgatc
    10741 cactatcaca gaatgctcta tcattggcgc ttaaactgaa acgtggataa tttcgttgtg
    10801 aatgttactt gcttgcgaag ttatcatttt gaaacctaaa tcaggtaatc acgcccatga
    10861 aacctcgtca gcgtcaggcc gccattctgg agtatctgca aaagcagggt aaatgctcgg
    10921 ttgaagaatt ggcgcaatac tttgacacca caggcacaac cattcgcaaa gatctggtca
    10981 ttctggaaca tgccggaacc gtcattcgta cttatggcgg agtggtgttg aataaagagg
    11041 aatccgatcc gccaatcgat cataaaacac tcatcaacac ccacaagaaa gagctgattg
    11101 cagaagctgc cgttagtttt atccatgatg gcgattcgat cattcttgat gctggcagta
    11161 ccgttttgca gatggtcccc ctgctctcgc gctttaataa catcacagtg atgaccaaca
    11221 gcctgcatat cgtcaatgcg ctatccgaac tggataacga acaaactatc ctgatgccag
    11281 gcggaacatt tcgtaaaaaa tcggcctcat ttcacgggca actggcagag aatgccttcg
    11341 agcatttcac cttcgataaa ttgtttatgg gcaccgacgg catcgatctc aatgcgggcg
    11401 taaccacgtt caacgaggtt tataccgtca gtaaggcaat gtgcaatgcc gcgcgcgaag
    11461 tgattttgat ggcggactca tcaaagtttg gccgtaaaag ccccaacgta gtttgcagtc
    11521 ttgaaagcgt cgataagcta attaccgacg caggtatcga tccagcgttt cgtcaggcgc
    11581 tggaaaagaa agggatcgac gtgatcataa ccggagagag caatgagtga agcactactg
    11641 aacgcgggac gtcagacgtt aatgctggag ttgcaggaag caagccgttt accggaacgt
    11701 ctgggcgatg attttgttcg cgccgccaat atcatcctgc actgcgaagg caaagtggtg
    11761 gtttcgggaa ttggcaaatc gggccacatt ggtaagaaaa tcgccgcaac gcttgccagc
    11821 accggcactc ctgccttttt tgtccatccg gcagaggcat tgcacggcga cctggggatg
    11881 atcgaaagcc gcgatgtgat gctgtttatc tcttactccg gtggcgcgaa ggaactggat
    11941 ctgattattc cgcgtctgga agataaatct atcgcgctgc tggcgatgac cggcaaaccg
    12001 acgtcaccgc tgggcctggc ggcgaaagcg gtgctggata tctccgtaga acgcgaagcc
    12061 tgcccgatgc accttgcgcc gacctccagc accgtcaata ccctgatgat gggtgacgcg
    12121 ctggcgatgg cggtcatgca ggcgcgcgga tttaatgaag aagattttgc ccgctcccac
    12181 cctgctggag cactgggcgc tcgcttgctg aataaagtgc atcatctgat gcgccgtgac
    12241 gatgccatcc cgcaggtggc gttaaccgcc agcgtgatgg acgcgatgct ggaacttagc
    12301 cgcaccggtt taggtctggt ggcagtgtgc gatgatcaac gactggtgaa aggcgtcttt
    12361 accgacggtg atttacgtcg ctggctggtt ggcggcggcg cactcaccac gccagtcaat
    12421 gaagcgatga cggtcggcgg caccacgttg caatcgcaaa gccgtgccat cgacgccaaa
    12481 gaaatcctga tgaaacgcaa aatcaccgcc gcaccggtag tggatgaaaa cggcaaactc
    12541 accggcgcaa ttaacctgca ggatttctat caggccggga ttatttaatc cttcagcccc
    12601 agacgtttcg ccagccgatg caggttggcg acgtcggttt ccagcatccg cgcacaggcg
    12661 gcccagttgt gatgattttg cgccagtgcc tgacgaatgg tttcacgctg gaacgcttct
    12721 gtcgcttcac gcaggttttg cttaacgata ggcaccgccg ccgcttctgg cggcggcaac
    12781 gtcacctcag gaaaagcaaa atgttgcgcc tcaagaatca cttcatcgcc gctgcgagtg
    12841 gctctcgaca gtactaccgc ccgatgaata gcatgttcca gttcgcgcac gtttcccgga
    12901 aaactgtagt gttgcagtaa atttcgcgcc ccggcactta ataccacgcg ggagagcccc
    12961 agccgcaaac gacactgctc gcagaaatac cccgccagca gaatgacatc atcgccccgc
    13021 tcacgcagcg gcggcaccga aagtggaaac acgctcaggc gatgaaacaa atcggcgcgg
    13081 aatcgccctg ccagcacctc ttcgcgtaaa tcgcggttag tcgccgccag cacgcgcaca
    13141 tcgacccgca aactgcggtc atcgccaacg cgctgaatat cgccatactg caacaccctc
    13201 agcagcttgg cctgcaatgc caacgacaac tcgccgatct catcgagaaa cagcgtgccg
    13261 ttatccgcca tttcaaactt cccgctgcgg ttactgatag caccagtaaa cgcccctttc
    13321 acatgcccga acaactcact ttccgccaca ctttccggca gtgcggcaca gttgagatag
    13381 accagcggat tcaccgcccg tggcgaggct tcatgaatcg ctttcgccac cagctcctta
    13441 ccggttcccg tctcaccgct aattaaaacg ttgagatcgg atgccgccac aatctcaatc
    13501 tcttttttca attgcgtcat gcctggggac aaaccaatca tctgcgtctg tttcaccgct
    13561 tcaaacggcg cggcatcgcc tggcagcata ttctggcttt ccagttgttc aatcagtaac
    13621 gcattactta acgctcccgc cgccagtgcg gcaatcagcc gtagctcttc gtcgctgaaa
    13681 acatcgaact gatcgggctg catcccgtcg agcgtcaagg caccgatcag gttttgcccg
    13741 gcaaacaacg gcaaaccaac gcaggcgtga accttcagac tctcctgccc tggaatcaaa
    13801 ccgtcataag gatcgggcaa ttcgctgtct gcgggaaagc gcaccacatc acccgcgcgg
    13861 gcaatcgctt ccagccgtgg atgaccttcc agcgcaaagc gtctaccgag tacatccttc
    13921 gccagcccgt cgatggcaag cggaataaac tgccgcgagt cgtaacgtag caacgcagac
    13981 gcatcgcact ccagcacctg acgcagcgtg gtgatcaggc gctgaaaacg atcctgatga
    14041 ccaatcccac gctgcaattc gatggcgata ttcgccagca catcaacgga aaaactcatc
    14101 tttgcctcac tgtcaatttg actatagata ttgtcatatc gaccatttta ttgatagtca
    14161 ttttgactac tcattaatga gcacaatttt atttatagag taaaaacaat tagataaaaa
    14221 actggcacgc aatctgcaat cagcaagaca tctttttaga acacgctgaa taaattgagg
    14281 ttgctatgtc tattgtggtg aaaaataaca ttcattgggt tggtcaacgt gactgggaag
    14341 tgcgtgattt tcacggtaca gaatataaaa cgctgcgcgg cagcagctac aacagctacc
    14401 tcatccgcga agaaaaaaac gtgctgatcg acaccgtcga ccataaattc agccgcgaat
    14461 ttgtgcagaa cctgcgtaat gaaatcgatc tgacggatat cgattacatc gtaattaacc
    14521 atgctgaaga ggaccacgcc ggggcgctga ccgaactgat ggcacaaatt cccgatacgc
    14581 cgatctactg tactgccaac gctatcgact cgataaatgg tcatcaccat catccggagt
    14641 ggaattttaa tgtggtgaaa acaggcgaca cgctggatat cggcaacggc aaacagctca
    14701 tttttgtcga aacgccaatg ttgcactggc cggacagcat gatgacttac ctgacaggcg
    14761 acgcggtact gttcagtaac gatgctttcg gtcagcacta ctgcgacgag catctgttca
    14821 acgatgaagt ggatcagacg gagcttttcg agcagtgcca gcgttattac gccaatatcc
    14881 tgacgccgtt cagccgcctg gtaacgccga aaattaccga gatcctgggc tttaacttac
    14941 cagtcgatat gatagcgacc tctcacggcg tggtatggcg cgataacccg acgcaaattg
    15001 tcgagctgta cctgaaatgg gcggcggatt atcaggaaga cagaatcacc attttctacg
    15061 acaccatgtc gaataatacc cgcatgatgg ccgatgctat agcccagggt attgcggaaa
    15121 ccgacccacg cgtagcggtg aaaattttca acgtcgcccg aagcgataaa aacgaaatcc
    15181 tgaccaatgt cttccgctca aaaggcgtgc tggtcggcac ttcgacgatg aataacgtga
    15241 tgatgccaaa aatcgccggg ctggtggagg agatgactgg tttacgcttt cgtaacaaac
    15301 gcgccagtgc tttcggctct cacggctgga gcggcggtgc ggtggatcgt ctttccacac
    15361 gcctgcagga tgcgggtttc gaaatgtcgc ttagcctaaa agccaaatgg cgaccggacc
    15421 aggacgctct ggagctatgt cgtgaacacg gtcgcgaaat cgcccgtcag tgggcgctcg
    15481 cgccgctgcc gcagagcacg gtgaatacgg tggttaaaga agaaacctct gccaccacga
    15541 cggctgacct cggcccacgg atgcagtgca gcgtctgcca gtggatttac gatccggcaa
    15601 aaggcgagcc aatgcaggac gttgcgccag gaacgccgtg gagtgaagtt ccggataact
    15661 tcctgtgccc ggaatgctcc cttggcaaag acgtctttga cgaactggca tcggaggcaa
    15721 aatgagcaac ggcattgtga tcattggttc gggcttcgcc gcccgccaac tggtgaaaaa
    15781 tattcgcaaa caggacgcct gtattccatt aaccctgatt gccgccgaca gcatggatga
    15841 gtacaacaaa ccggacctca gccatgttat cagccagagg caacgtgccg atgaccttac
    15901 ccgccagacg gcgggtgaat ttgccgagca gtttaatctg cacctgtttc cgcacacctg
    15961 ggtaacggat atcgatgccg aagcccatgt ggtgaaaagt cagaataatc agtggcaata
    16021 cgacaagtta gtgctggcaa ccggtgccag cgcctttgtc ccgccagtgc ccgggcgtga
    16081 gttaatgctg acgttaaata gtcagcaaga gtatcgcgcc tgtgaaacgc aactgcggaa
    16141 tgcccgacgc gtgttgattg ttggcggtgg cttgattggt agcgaactgg cgatggattt
    16201 ttgtcgtgca ggcaaagcgg tcacgctaat cgacaacgct gccagtattc tggcagcgtt
    16261 aatgccaccg gaagtaagca gccgcttgca gcatcggttg acggagatgg gcgttcatct
    16321 gctgttaaaa tctcagttgc aggggctgga aaaaacggat tctggcattc tggcaacgct
    16381 ggaccgccag cgcagcatcg aagtggatgc ggtaattgcc gccaccggac tgcgcccgga
    16441 aaccgccctg gcacgacgcg ccgggctgac gattaatcgc ggcgtttgcg tcgatagtta
    16501 tctgcaaacc agtaatgccg atatttatgc gctgggcgat tgcgcggaaa ttaacggtca
    16561 ggtattgccg ttcctccagc cgattcaact tagcgcgatg gtgctggcaa aaaatcttct
    16621 cggcaataac acgccgctga aactcccggc gatgctggtg aaaatcaaaa cgccggaatt
    16681 accgctgcat ctggcaggcg aaacccagcg tcaggattta cgctggcaaa ttaataccga
    16741 acgccaggga atggtggcgc gcggtgttga tgatgccgac cagcttcgcg cctttgtggt
    16801 cagtgaggat cggatgaaag aggcgtttgg attgttgaaa acgttgccgg tgtaggcgag
    16861 ctacagtgcc ccaaatgtcg gatgcggcgc tgacgcgtct tatccgacct acagggaacg
    16921 catgtgttga tcacataata cgtttatgtc gcatcctgca ctgtcccaaa atgtcggatg
    16981 cgacgctggc gcgtcttatc cgacctacgg ggacgcacgt gtaggccgga taaggcgttt
    17041 acgccgcatc cggcaatggt gtccaaatgc aacacgcttt atccgttctg gacttcaccc
    17101 gctaaccagc gcgccgcagc aataaccccc tgccccagag acaatccacc atcgcccgcc
    17161 ggtaaactct gcgggaagag caacgtaaaa tcagcaagat aatgcgccag acgtgcacgc
    17221 agcaaacggt tatgaataac tccgccgcta aataccagcg tagtgatacc ccgcatcgtg
    17281 gcctgctcac gcatcaacgc ggcaaaaccc tgcgccagcg catcatgaaa cgcccacgcg
    17341 cgttgattaa ccggagcctg ccagctcagc cactgctgcc agaaagtggc gagatccagt
    17401 tgattgtcca ccagcggcat cgtcaccgga tgcgtcactc cgtcgcacga agctgcgagc
    17461 gcctccagag cacaagccgc ttcaccttca taacttaacg tggctggcgc acagcccagt
    17521 cccgccgcca cagcatcgaa caaacgccca cacgatgacg ccagcggcgc gttaattcca
    17581 cgctcaatgg cccgcgccag cacgctccag ttttgctgtt gcacacttgc cgtttcaggg
    17641 taattctgcc actccggcac aaagcgcagg cactgcgcca gcaggttacg ccacggctgc
    17701 ttcgccgcca aatcgccccc agcaagcgcc actgcaggca agccgcccag gtgctggcat
    17761 tcgcgatagt tcacccgcag gcactcgccg ccccacaaag cgccgttctc ccccataccg
    17821 ataccgtcga gcgtcaacgc aatgacatca ccgccatcca gtggccagtg atgttctgcc
    17881 agacacgccg ccgcatgagc atgatgatgc agcaccgttt gcgtcggcag attcatctca
    17941 cgcgcccact ggctggagac atagcccgga tgcgcgtcat gcacaacgta ttgcggggtg
    18001 aaatcgtaga tgttttgcat caggcgtaac gcttcgcgcc actgcatctg gatgccatcg
    18061 tcacttaaat cgcccagatg ctggctcaac accgcttgtt caccgcgcac caggcagaag
    18121 gtatttttca gatccgcgcc gagacacagc acaggcggaa catttttaaa gcccggaggc
    18181 aaagccagcg catccggcac atacccccgc gaacggcgca gcatttcgcc gctttcgcgc
    18241 accaccgaat catccatccg ctgcacgatg tcgcggttat gtatcaagaa tccgtcggca
    18301 atgccctgca aatccgccag cgcctgttcg ttgctgatag ccggtggttt accgctcagg
    18361 ttgccggagg tcatcaccag cgggcattgc agttcctgta acagcaaatg ctggagcggg
    18421 ttcgcaggca acatcacccc aacttcgtta aggccagggg cgatatcatc acaaagctca
    18481 ggaacgtatt ttttatccac cagcacaatc ggcgcggcgg gtgtggtaag caactgacgg
    18541 gcagcgtctg gtaaaccttc tgccactggc aacataaccg ccagcggttt cgccgggcga
    18601 tgtttgcgcg cccgcagtgt cgccaccgcg ttactgttac gcgcatcgca ggcgagatga
    18661 aatccgccaa tccctttgat ggcgacaatg ttgcccattt ttaactgtgc gatagctgcc
    18721 tgtaatgccg cctcttgttc tgcatgttca ccatgactta cccattcaag atgggggcca
    18781 cactccgggc aggccaccgg ctgggcgtgg aagcgacgat cgagcgggtc acggtactct
    18841 ttgtcacagg ccggacacag cggaaacgcc gccatcacgg taaacgggcg gtcgtaaggc
    18901 atggcgcgaa taatggtgaa acgcggacca cagtgagtac agttgataaa cggataacgc
    18961 cagcgtcgtt cgcctggggt attcatttcg gcaagacaag cagggcaagt cgcggcatcc
    19021 gggacaattt gcgtattcat gacgccaccc gcgctctggc ggatggtgaa ctcagtgggc
    19081 agttgtgacc agataaacgg ctcacgctcg acgctatcaa tacgcgccag tggcgggcag
    19141 tgctgatgca actgcacaag aaacgtttcc ggatcttcca gcagccggac ttctacgcca
    19201 tcgccgtcat tacagacatc gccgtgaaga tttaattgct gtgccagttg ccagacaaac
    19261 ggacgaaaac cgacgccctg cactttgcca cgaatacgca gttggacacc gcaagatgtg
    19321 ttttttgcca ttgagttatt cccgccatca tgaattgcgt aacccgccca gccggacacg
    19381 acagcgtcgc atccggcagt cacaggtcgg cgatactgcc cgcttcgtat tctacgaata
    19441 tttccgggaa ttcctttgat gccagaacag ttcagtaaga tttttagaac atcagcgccg
    19501 tacggcgacg tttttctgcg ctcagttgtt caagtttatt acgatcgaca caaatcagcg
    19561 catgggtcgg gcaagccgcc atacacgccg ggccgtcttc acgatggttg cacaggtcgc
    19621 atttattggc ttcggctttg tcagcccgta cattcagccc cgcgccgctg ttgcgaatca
    19681 ccggacgtac caccacttcc atcgcgccat acgggcaagc cacaacgcag gttttgcaac
    19741 caatacaacg ttcctgcatc acatgaacaa accctttatc acgactgata gcaccattcg
    19801 gacagacgtt agcgcacggt gcatcttcac actgacggca aactgtcgcc gtggaaatgt
    19861 tcacaccttt aatgacatgg attcgcggta aaaaagtttc cggggtcagc gatgcacagt
    19921 cctgattttc ctgatgagaa accacgcacg ccacttcaca ggtacggcaa ccaatacatt
    19981 tactcgcgtc agcaatgatg aaacggttca tcaaattctc cagcaatgac agttaatgcg
    20041 ccgatacatt cacaaatcat gccagttttt aatttactgt tatttaaaga aattaaattc
    20101 tgtaatgcag gaaaaacgat gtcatcgaca ctagtgacga tgacatgtga tgacaatgtt
    20161 tatcgcgaaa gagcaatgag ggagtcgcgg cggatcagtt ttccgctgaa ggttttcggc
    20221 ggtgtgtaag caccaccatc gagcataaat atcagccgcc caattatttc ctgaatcatc
    20281 tcagtcaccg gaatttttac gctggagagc gccggaacgg tgtagggggc aatagcgata
    20341 tcatcgaatc cgataactga cacctgctct ggcaccgcta cgccgcgctc gtgtaacgct
    20401 ttcatcgcac ctatcgccat atcgtcgtta ctggcaacta acgcgctaaa tttagcccca
    20461 cgttcgagca acatttctac cccttcggcc ccgctggcag gcgtccattt accgttagcg
    20521 ataagttttt cattgagcgc aataccatgc tgcgccagcg cgtctttata cccggcaaga
    20581 cgttcaacgc tggtggggga atccatcgaa ccggtaagga aagcaatctc ctgatgtccg
    20641 gcgtttatca actctgccac ggcgttaaag ctggtctgtt tatgatcgca ccagacgcta
    20701 tggctgctgt ttttgcgcag gcggcgatta agcaccatta tcggctgact gtgtgcgtca
    20761 atgatgtcat caatctcatc cacgcttaaa aaacgcgggt aaatcataat ggcgtcgcag
    20821 cgcaaatcca gcagatactg aatcgcctgg cgctcttctt cggcgctgtg tttaccatct
    20881 gccaatagca actgtcgccc tttctcttcc gccattcgcg cggcatgaaa gagtaattca
    20941 ctaaaataaa tgccgtggta aagcgtgttg gtcacgacca gccccagcgt ctgagtactc
    21001 ttcgccgaca gattgcgcgc cagcaagttt ggacggtaac cgctctcttc taccgcctga
    21061 aacacgcgat ctttagtctc ctggctgacg tagccattac ctgaaagcac gcgggaaacg
    21121 gtcgcttttg aaaccccggc gcgcttcgcc acttccagca tcgtcgtcat cattttcatc
    21181 cctttacacg caatcaacgc agtgtactgc accgtttgcc gattgtcctt gcgcaatcgg
    21241 cgggaaaaat attcaggtga ccggtttcac aaatataaaa aatgaacaat taactctctt
    21301 gcttattcag tgacaactat tcatgatttt gtgaaaccgg tttcctaatt ccgtttcagc
    21361 atcggcattt ttccgtcacg tcgactgata acaactacat ctaccctact gataacagga
    21421 taaaatccga tggccaaaaa ttatgcggcg ctggcacgct cggtgatagc ggcactgggc
    21481 ggcgttgata acatctcggc agtcacgcac tgtatgacgc gtttgcgctt tgttatcaaa
    21541 gatgaccagc ttatcgacag tccgacgttg aaaaccatct ccggcgtgct cggcgtggta
    21601 cgtagtgaca accagtgtca ggtgattatc ggcaataccg tttcacaagc ctttcaggaa
    21661 gtcgtcagcc tgctgccggg agatatgcag cccgcacagc ccgtgggtaa acccaaactt
    21721 acgctacgtc gcataggtgc ggggatcctc gatgcgctga tcggcaccat gtcaccgctg
    21781 atcccggcga ttatcggcgg atcgatggtc aaactgctgg cgatgatcct cgagatgagc
    21841 ggcgtgctga caaaaggatc gccgacctta accattctga atgtgattgg tgacggtgct
    21901 ttcttcttcc tgccgctgat ggtcgcggct tcggccgcca tcaaatttaa aaccaatatg
    21961 tcgctggcga ttgccatcgc gggtgttctg gtacatccga gttttattga actgatggcg
    22021 aaagcggccc agggtgaaca tgtcgaattt gccctgatcc cggtcaccgc ggtgaaatac
    22081 acctacacgg tgatcccggc gctggtgatg acctggtgcc tgtcatatat cgaacgctgg
    22141 gtggatcgca ttacaccggc ggtgactaaa aacttcctca agccaatgct gattgtgttg
    22201 attgccgcac cgctggcaat cctgctgatt ggcccgattg gtatctggat cggtagcgcc
    22261 atttcggcgc tggtttacac cattcatggt tatctgggct ggctttcagt cgccattatg
    22321 ggcgcgctgt ggcctctgct ggtaatgacc gggatgcacc gcgtctttac gccaaccatc
    22381 attcagacca ttgccgaaac cggcaaagaa gggatggtca tgccgtcaga gatcggcgct
    22441 aacctgtcgc tgggcggttc atcactggca gtggcgtgga aaacgaaaaa cccggaactg
    22501 cgtcagacgg cgctggctgc ggcggcatca gccattatgg cggggatttc cgaaccggcg
    22561 ttatatggcg tggcgatccg cctgaaacgt ccgcttatcg ccagtcttat cagcggtttt
    22621 atttgcggcg cggttgccgg tatggcgggg cttgccagcc actcaatggc agcgccgggg
    22681 ctatttacca gcgtgcagtt cttcgatccg gcgaatccaa tgagcatcgt ctgggtgttc
    22741 gccgtcatgg cgctggcggt cgtgctgtcg ttcatcctca cactgttgct cggctttgag
    22801 gatattcctg ttgaggaagc gactgccgag gcgcgaaagc atcagagcgc acaaccgacc
    22861 gtcgccaaag aagtaagtct taattgagga tgaaaatgtc agtatttcca gaaggttttt
    22921 tatggggcgg cgcgcttgcc gccaaccagt ctgaaggtgc gtaccgggaa ggtggcaaag
    22981 ggctgaccac cgtcgatatg atcccacacg gcgagcatcg aatggcggtg aaactggggc
    23041 tggaaaaacg ttttcagttg cgcgatgacg agttttatcc cagccatgag gcgacggatt
    23101 tttatcatcg ttataaagaa gatatcgccc tgatggcaga gatgggattc aaagtgttcc
    23161 gtacctcaat tgcctggagc cgcctcttcc cgcagggcga tgaactgacg cccaaccagc
    23221 agggcattgc tttttaccgt tctgtctttg aagagtgtaa aaagtacggt atcgaaccgc
    23281 tggtcacgtt gtgccacttc gatgtgccga tgcatctggt caccgaatat ggctcctggc
    23341 gtaaccgcaa gctggtggag tttttcagcc gctacgcccg gacctgcttt gaagcatttg
    23401 atggtctggt gaaatactgg ctcaccttca atgaaatcaa cattatgttg catagcccgt
    23461 tctccggcgc gggtctggtg tttgaagaag gcgaaaatca ggatcaggtg aaatatcagg
    23521 ctgcgcatca ccaactgatt gccagtgcat tagccaccaa aatcgcccat gaggttaacc
    23581 cgcaaaatca ggtgggctgt atgctggcgg gcggtaactt ctacccttac agttgcaagc
    23641 cggaagatgt ctgggcggcg ctggagaaag atcgggaaaa cctgtttttt atcgatgtgc
    23701 aggcgcgggg cgcgtatccg gcttactctg cccgcgtatt ccgcgaaaaa ggggtaacca
    23761 tcgacaaagc accgggcgat gatgagatcc tgaaaaacac cgtcgatttt gtctctttca
    23821 gctattacgc ctcacgctgc gcctcggcgg agatgaacgc caacaacagc agtgcggcga
    23881 acgtggtgaa atcgctgcgt aacccatatc tacaggtaag cgactggggc tgggggattg
    23941 atccactcgg tctgcgtatc accatgaaca tgatgtatga ccgctatcag aagccgctgt
    24001 ttctggtgga aaacggcctc ggcgcaaaag atgaacttgc tgccaatggc gagattaacg
    24061 atgactatcg catcagctat ttacgcgaac atatccgcgc aatgggcgaa gcgattgcag
    24121 atggcattcc gctgatgggc tacaccactt ggggctgtat tgatttggtt tccgcctcaa
    24181 cgggtgaaat gagcaaacgc tacggttttg tctacgtaga ccgtgacgac gcaggcaacg
    24241 gtacgctggc gcgcacgcgt aagaaatcgt tctggtggta taaaaaagtg attgccagta
    24301 atggtgagga tttagagtaa gtaacagtgc cggatgcggc gtgaacgcct tatccggcct
    24361 acatgtcgca cgaaccaggt aggcccgata agcgcagcgc atcgggcaat ctggcgtttg
    24421 ctatcagttc tgattccctc atcaagaaca tccctgtcct gattccttac tgaaaaacta
    24481 ctcttcttcc accgctaact gcgcgaatcc gccattccct tcccagcctt ccagtcgttg
    24541 ataaacggtt tctaccgcat ctttaatcgg ctgggtcatc gggtagtaaa agccgacgat
    24601 atccggctgg atgccgagga aaatcacttc gccaatgtct tcttttaact ggtcgataag
    24661 gtagttcaac ggcatgttat gggtagtcat cataaacatc tcggcgatat catccggatc
    24721 gatgatgcgg atctcgccgg ggtttaaccc catatccgtg gcgtcgacaa tcagcagccg
    24781 tgtcgggcgc agttcgcgga tggcgacgat gtcgttttcc ggagcgctac cgccgtcaat
    24841 caccacccag ttacctttcg gcgcggcggc gcacttttcc gccagcagcg ggcctgcgcc
    24901 atcatcgccc atcatgctat tgccaacaca aagtaaaacg tcagtcacgc agtctcctca
    24961 ccatcaaata gatggcgttt tcctgatgaa tatcatgcag catacttaac agcattgtac
    25021 tccattcttt ctgctgcgaa gtttgcgctg cccgggcttt atcgaatgcg ttagccagca
    25081 tcggcacatg gttaatgtcg atgacgatct caccatattt cggcacgcct tccattttgc
    25141 ggcgggcttc gctgcccgcc tccagagtgg caatccatgc cagatagtca tcccacgggc
    25201 aggtaagcgc cgcttccagg caatcgataa ccccaaggtg gtgaccaatc gccaggctgt
    25261 aatagaccac ctgctgcgcc tcggcgggcg tagcatcgtt ctcatcaata aatttacggc
    25321 tcagttgact gaacaccacc ttttcactca tcggatacgc gcctcttcaa caacatgatt
    25381 cagatggctg acaatctcgt tcagacgcgg atcgttttcc gcttccagcc agcgtgcaac
    25441 ctgttcttcg ccctgcccta actgtgtaag gtaatcatcg gcaatctgac gaccgtaacg
    25501 ataacccgcc aggcggcgtg cttcgcgatc cactttcaca cgcagcggct gcaccatatc
    25561 gccatgcagg atctccgccg gttgttcatc ctgctcaccc ggcccacggg cgtgaatttt
    25621 ctgctccagc aggccgagcg ccattgcaaa gccgtatagc gtagcggcag gcgttggcgg
    25681 gcagccaggg atgtagacat ctaccgggac gattttatcc gtaccgcccc acacgcagta
    25741 gagatcgtgg aagatcccgc cactgttacc gcaggcaccg taggagatac agattttcgg
    25801 gtccggcgcg gactgccacg cacgcagcgc aggggatcgc attgcacggg tgaccgcgcc
    25861 ggtaaacagt aaaatatccg catgacgcgg tgaaggaacg actttaatgc cgaagcgttc
    25921 tgcatcaaac agcggcgaaa gcgtggcgaa aatttcgatt tcgcagccgt tgcagccacc
    25981 gcagtccacg cggtaaacgt aggcagaacg tttgattttt ttcagtaacg acgccttcat
    26041 gctggcgatg gattcatcca ccgtcatcgg gaccggaatg ccgttggcgt cacgggggcc
    26101 taataaattg ctcatcagat ggcctctttc atatggcgag tcagttcaat acggtcggac
    26161 ggcaccaggc atttctggcg cttacattcc gggcaagtct caaagctttc gcggtggttt
    26221 tccgcgcggc tgtcaccatt gtgcttaagc agcgcaatgg cgtagtcgat ctctttctgg
    26281 acggcgaaag gacgattgca gacgcggcag ttgcacagcg cgaagcggga ctgttggagg
    26341 aagtcttctt tcttccacac cgccagttcg tactcttgcg acagtttgat cgccgccgtc
    26401 gggcaaactt cttcgcagcg tccacagaag atgcagcgcc cgagattaaa ctcccaggcc
    26461 agctctcctg tggcgaggtc ggtttcaacc gttaaggcgt ttgacgggca ggcattgacg
    26521 caggccgcgc agccgatgca ctgctgcggg ttctgctctg gcttaccacg gaagttttta
    26581 tcaaccgcaa tcggctccag cggataagac gaggtcgccg tgccggtttt gatgactttt
    26641 ttgataaagg taaacatggc gattccttat ttcagcggcg agtttttacg ctcaatgctg
    26701 taacgctcga gttctttgta cggcaccact ttgctcttct tcttacgcac atcgaccacg
    26761 gtcatgcggt cggtacagga gtagcaaggg tccaggctac cgataatcag cggcgcatcg
    26821 gaaacggtgt tgccgcgcag catgtagcgc agggtcggcc agttcgcgta ggtcgcggca
    26881 cggcagcgcc agcggtacag cttctggttg tcgccggtca tgctccagtg gatatcgtcg
    26941 ccgcgcggcg cttcggcaaa gcccagcgca aaacggtgcg gaatgtaggt aaaaccttct
    27001 accatcaacg gaccgcccgg caggttatcc agaccgtagt cgatcatgtt cagcgcggta
    27061 tagacctcgt tgattcgcac tttcagacgg gaaataacgt cgcagccctg ctcgctgtgg
    27121 acttccattg gcagcaggcc ataaccgaca aacgggtgat cggcgcgagt atcgcgggcg
    27181 tgaccgctgg cgcgaaccat cgggccaacg ttactgaaat cgcgggcgat ttccgggtcc
    27241 agacgaccaa tgccgacggt gcgctgttcc atgttcggtg tgctcagcag cacatccacc
    27301 agctcctgca cttcacgacg catctgttgt gccagctggc gggtctggat catgtcgtct
    27361 ttcagcagat cgcgacgaat cccgccgatc aggttcaggc cgtaggtttt acgtgcaccg
    27421 gtaaggatct ctgccatttt catggaggtt tcacgcacgc ggaagaactg cataaagccg
    27481 gagtcgaagc cggtaaagtg acaggcgagg ccgagattga gcagatgcga gtgcaggcgt
    27541 tccacctcca acagaatggc gcggatcatc tgcgcacgtt ctggcacctg aatacccatc
    27601 gcgttttcca ccgacgtggt gtaggcggtg ctgtgagcaa agccgcagat cccgcacaca
    27661 cggtcagaga ggaaggtcac ttcgttgtaa cccatacggg tttccgccag tttttccatg
    27721 ccgcgatgga cgtagaacag gcggtagtcg gcgtcgataa tgttttcacc atcgacaaac
    27781 agacggaagt gacccggttc gtcagaagtg acgtgcaggg gaccaatcgg cacgacgttg
    27841 tttttcttgt cgcccagttc gttgatgaac tcgtaggttt cagcatcggt agtcggtgcc
    27901 ggacgctgac gataatccat gctgtcttta cgcagaggat aaagttcatc cggccagtca
    27961 tccggcagca ccagacggcg ttcatccggc agaccaaccg gaatcaaacc gtacatatcg
    28021 cgcacttcac gctcgcccca cactgccgcc ggaacgcgcg gcgtcacgga aggatactcc
    28081 ggtttgttgg cgtcaacttc gacgcgcacc gttacccaac acttagtccc cttctccatc
    28141 gacagcacgt aataaacggc gtaatgacca ttcagtttgc gttcgtcgtt accaaacagc
    28201 accgacagcc agcccccctg cttgtagtag agaaactcca ccacttccgg caggtagttc
    28261 accttcacgg tcacggtcag ctgatctttg gtctgccagg cgtggtccag cacgacgccc
    28321 ggaaatgcct cattcagcgc ggcgagataa tgttgaccta atttttcttc agacatgctc
    28381 aaactctctt taatcacgcc gccagcaagg agacgaacgc taaaaatgca aagccaaaac
    28441 cagcccaggt aatgcgtgga gtaatatcaa gacgcaaacg ggccatgctg ttttcgaaca
    28501 gcgcgataac cagtacgccg accaccagtt tgacgatggc aatcaccagc gccagcagca
    28561 gcccaccgac ggtgaaggtt tccatttgtc cccacggaat aaacaccccg acgaacatct
    28621 gcaacaccac cagctgtttc aggctgatgc cccatttcat gacgccaaag ccgctgccgc
    28681 tatactcaga gagcggacct tcctgcaact cctgctcggc ttccgccagg tcgaacggca
    28741 gtttgcccat ttcgataaaa gtggcgaacg cacaggcaca aagcgccagc accagcggga
    28801 tgctctggct cagtggccag tgataaacgg tgtcggtgat gttgctgatg ttggtggaac
    28861 cggcaacctg tgcggcgacc cacagaccaa gcagcagcat cggttcaacc aacacgccaa
    28921 gcatcgcttc acggctcgcg ccgatagcgg taaacgggct accagtatcc agaccagaaa
    28981 tggcaaagaa gaaacgcgcg atggcgaaga gatacagtaa ggtgatcaaa tcgcccagtt
    29041 gcggcagcgg agagccgacg gtcaccaccg gcagcgcagt ggcgatagtc agcatgacgc
    29101 ccaccatcac atacggcgtc aggcggaaca cccagccgga ggcatccggg ccaacgctct
    29161 gacgccccag cagtttgata atgtcgcgat actcctgcaa cacgcccggc ccacgacggt
    29221 tatgcaagcg ggcgcgcgct acgcgggtaa taccggagag cagcggcgca acggcaaata
    29281 acaccagcgc ctgaattaac ggatataaaa cactcattct caggctcctc gtgaaacaat
    29341 aatcaccacc agtaccgcca gttcaaccag cgccatccgg cggaacagca acgcactccc
    29401 ctcgcactgc cagcccggca ccagcgacac cggattcagc catttgcgta gtttcagcac
    29461 cggcgcaaac gcctgtttca ccggcatggc aaaaccgtga gcggtaatca ccattgattt
    29521 ttcatgatcg tagccgcaca cccaggccgc gccgcgggaa cgcgacggca aacgatcgcc
    29581 tttgcaaatc gccataatga tgaatggcag cagcgggcag gcaatcagca gcaacgtgat
    29641 catgggttga gaaacggtgg tgttagcagg ctccagcggc agaggtacag cagcagagag
    29701 catcggcagt agccacggcg cagcaacacc gccaattacg cagcaaatcg ccagtgccac
    29761 tacgcttacg ctcatcagga gcggcgcaca ggtggcgttt tcggcctctt tggtacgcgg
    29821 cgcgccgagg aaagtaacgc cataaacttt cgccatacac atcaccgcca gcgcaccggt
    29881 aattgccagc cccacggcga gcagcggccc gagaagacgg gcaacaaacg cgccactatt
    29941 gctcagtttg aagaaggatt gatagataac ccattccccg gcaaacccgt tcagcggcgg
    30001 cagcgcagcc attgccatca gcccgactaa catggcgatg gagataaccg gcattttctt
    30061 gccaatacca ccgagttttt cgatatcgcg atgaccggta cggaaccaga cgctccccgc
    30121 accaaggaac agtacgctct tgaacaggct atggttaagc agatggtaca gaccaccaac
    30181 cagaccaagg gcaatcagcg ccggttgttc gagcgcgata cccgttacgc cagcgcccag
    30241 ccccagcagg atgatgccga tattttccag ggtgtggtaa gccagcagac gttggatatt
    30301 atgttccatc aacgcataca gaccgccgac gaatgcggtg atcatgccga gtaccagcag
    30361 tgcgatcccc caccacagcg gtgcattacc gcccagtagt gacagagtta aaatgcccag
    30421 caggccaatt ttcatgacta ccgtggaaaa cagcgcggca gctggcgcag aggcgttggc
    30481 atgtgcctgt ggcacccagc cgtgcagcgg aataatcccg gccagcaggc caaagccaat
    30541 cacaccgagc agccagatat cggaaccgag cggcagctgt tgcatacgca tatccagcag
    30601 gcgcagatcc agcgtgccgt aacgctgcca cagcagccag caggcaatcg ccagcagcag
    30661 agtgccaaga cgccccagcg caaaccacag tttgccctct ttgctgttgc tggtgaggaa
    30721 caccgcacac agggccatga tttcggccat taccacgaac atgccgaggt tgctggcgat
    30781 gacggcgcag acggcggcag ccatcaacat attgatctgc aagccgttgc acttcacctg
    30841 cgcgtggcga tgccagtcaa tgttgtagag gctgacaaac agaccgcata ggccgagcgt
    30901 aatcagccaa atcgcattaa gcggagagat ttgcacatcg tagcttacca gcgacagcgc
    30961 accgctcacg ccaaccgcgc cagtcagcac tgtgaagccc gcggctgccg tatacaggct
    31021 accaaccgcg ccgccaattc cggctatcca gccgcttagc gccttttgaa aagaaaagag
    31081 aaatgccaga acagcggcgg cgacaaacca cgccacgccg ctattgatca gggaaattgc
    31141 gctcatttag cctctccact ttgagcctgc tgaaacaagg tgagatcgcc aaaatccgtg
    31201 ttaaaagtca gctcacgctt acgtttgctg acgcgggcga tatcggtgtt atccaccaga
    31261 tgcagggctt tagtcgggca catccgcacg caggccggac cttgttcatc aaagctacaa
    31321 aggtcacatt tgacggcgat cgcgcgaata cctggcaccc agtcaagcaa tgtgctgaca
    31381 cgcgccggag caggcggtgc cggtggcgct ttcggggtat tggcgtttgc cggaatatcc
    31441 agcggacggc tgccggaaaa ttcaattgcg ccaaacgggc aggcgatgcc gcacagtttg
    31501 cagcttacgc acagactttc attcaactgc acggccccat caacgcgggt gatggcgtta
    31561 accgggcaaa ccaccgcaca gggcgcatct tcacagtggt gacagagctg cggcgcagat
    31621 tctttttcat tgagcatcac tcgcaggcgc ggcattgatt gcaggccgtg ctggcgatgc
    31681 gtctctgaac aggcggcctc acaggtgtgg cagccgatac agagcgtgga gtcagcaatt
    31741 acaaaacgat tcaccaggca ttcctcaggt gattgtcatt tttgacgaaa acatgccgat
    31801 gaaatgtcat tttcgacact catcgacacg cccatcccca aacaggcgta acgcctgcaa
    31861 aacgggcaaa gcctcagctc atgctgccgg gctttgtccc tttaccagtt ggcttaaatt
    31921 caccggcaca ttgttttcaa tggcggtgta taagctgtcg taaacgccag cgattttttc
    31981 gagatagtgt tccagaacct gttcgcgatc gcggatgttg accttgccat caatcatgcc
    32041 gtcaatgctc agttgcgcct gcgcaatccg ctgtttgtct tcaccatctt ttgtttcgac
    32101 gtaatactcg atggtgtggc tgttaaagcc atccgccagg gtgacgtaaa tacgaaaatg
    32161 ttcaaaaagg acgaaacaga gttctttgtc cggcgcgcag ctcatggcgt gatgcaggcg
    32221 cgctttcgat aacgtgattc cctgaaccag cgaattgcag tagatgtgcc actggtcctg
    32281 taggcgacga tgccgctgtg cgatgtaatc ggctttctcg cttatttccc aaatagtcat
    32341 tgtcaggtta cccgtttaac agagatgcca gctttaagca ttttttgtgc caacttttaa
    32401 tttattgtta ttaaagcgat ttttaagcta aagatgaatt tcgtcgccgt gtcgacgtgt
    32461 catttcgaca tcatcgacat tattcaccgc agggataatc aacactggca caattattgc
    32521 ttgtagctgg caatagttaa tgggaggcga tatgcacgaa ataaccctct gccaacgggc
    32581 actggaattg atcgaacagc aggccgcaaa acacggcgca aaacgcgtaa ctggggtctg
    32641 gctcaaaatt ggcgcatttt cttgtgtcga aaccagctct cttgcctttt gttttgatct
    32701 ggtttgccgc ggcagcgtgg cggaaggttg taaactgcac ctcgaagaac aagaggccga
    32761 atgctggtgt gaaacatgcc aacagtatgt gacgctactg acccagcgcg tccgccgctg
    32821 tccacagtgt catggtgaca tgctacagat tgtggcagac gacggtttac agattcggcg
    32881 gatagaaata gaccaggagt gagcgatgtg tacaacatgc ggttgcggtg aaggcaacct
    32941 ctatattgag ggtgatgaac ataaccctca ttccgcgttt cgtagcgcgc catttgcccc
    33001 ggcggcacgc ccgaagatga aaatcaccgg cattaaagcg cctgaattta cccccagcca
    33061 gactgaagaa ggcgacctgc attacggtca tggcgaagcg ggcactcacg cgccgggcat
    33121 gagccagcgt cggatgctgg aagtcgaaat tgacgtgctg gacaaaaata accgtctggc
    33181 tgaacgcaac cgcgcgcgct ttgctgcccg caagcaactg gtgctcaacc tggtttccag
    33241 ccctggttcc ggtaaaacca ccctgctgac ggaaacttta atgcgcctga aagacagcgt
    33301 tccgtgcgcg gttattgaag gtgaccagca aaccgtgaac gatgccgcac gcattcgcgc
    33361 taccggcacc ccagcgattc aggtgaacac cggtaaaggc tgccatcttg acgcacagat
    33421 gattgccgac gccgcgccgc gcctgccact ggacgataac ggtattctgt ttatcgaaaa
    33481 cgtcggtaac ctcgtttgcc cggccagctt cgatctcggt gaaaaacaca aagtggcggt
    33541 gctttccgtt accgaaggtg aagacaaacc gctgaaatat ccgcacatgt ttgccgccgc
    33601 ctcgctgatg ctgctcaaca aagttgactt gctgccgtat ctcaatttcg acgttgagaa
    33661 gtgcatcgcc tgcgcccgtg aagtcaatcc agaaattgaa atcatcctta tttccgccac
    33721 cagcggcgaa gggatggacc agtggctgaa ctggctggag acacagcgat gtgcataggc
    33781 gttcccggcc agatccgcac tattgacggc aaccaggcga aagtcgacgt ctgcggcatt
    33841 cagcgcgatg tcgatttaac gttagtcggc agctgcgatg aaaacggtca gccacgcgtg
    33901 ggccagtggg tactggttca cgttggcttt gccatgagcg taattaatga agccgaagca
    33961 cgcgacactc tcgacgcctt acaaaacatg tttgacgttg agccagatgt cggcgcgctg
    34021 ttgtatggcg aggaaaaata atgcgttttg ttgatgaata tcgcgcgccg gaacaggtga
    34081 tgcagttaat tgagcatctg cgcgaacgtg cttcacatct ctcttacacc gccgaacgcc
    34141 ctctgcggat tatggaagtg tgtggcggtc atacccacgc catttttaaa ttcggcctcg
    34201 accagttgct accggaaaac gttgagttta tccacggtcc gggttgcccg gtgtgcgtac
    34261 tgccaatggg tagaatcgac acctgcgtgg agattgccag ccatccggaa gtcatcttct
    34321 gtacctttgg cgatgccatg cgcgtaccag ggaaacaggg gtcgctgttg caggcaaaag
    34381 cacgcggtgc cgatgtgcgc atcgtttact cgccaatgga tgcgttaaaa ctggcgcagg
    34441 agaatccaac ccgtaaagtg gtattcttcg gcttaggttt tgaaaccacc atgccgacca
    34501 ccgccatcac tctgcaacag gcgaaagccc gcgatgtgca gaatttttac ttcttctgcc
    34561 agcacattac gcttatcccg acgctgcgca gtttgctgga acagccagat aacggtatcg
    34621 acgcgttcct cgcgccgggc cacgtcagta tggttatcgg cactgatgcc tataatttta
    34681 tcgccagcga ttttcatcgc ccgctggtgg tcgctggttt cgaacccctt gatctactgc
    34741 aaggcgtggt gatgctggtg gagcagaaaa tagcggccca cagcaaggta gagaatcagt
    34801 atcgtcgggt ggtaccggat gccggtaacc tgctggcgca acaggcgatt gccgatgtgt
    34861 tctgtgtcaa cggcgacagt gaatggcgcg gcttaggcgt gattgaatct tctggcgtgc
    34921 acctgacgcc ggattatcaa cgattcgatg ccgaaacaca tttccgcccg gcaccgcagc
    34981 aggtctgcga tgacccgcgt gcgcgttgtg gcgaagtctt gacgggcaaa tgtaagccgc
    35041 atcaatgccc gctgtttggt aacacctgta atcctcaaac cgcgtttggt gcgctgatgg
    35101 tttcctccga aggagcgtgc gccgcgtggt atcagtatcg tcagcaggag agtgaagcgt
    35161 gaataatatc caactcgccc acggtagcgg cggccaggcg atgcagcaat taatcaacag
    35221 cctgtttatg gaagcctttg ccaacccgtg gctggcagag caggaagatc aggcccgtct
    35281 tgatttggca cagctggtag cggaaggcga ccgtctggcg ttctcaaccg acagttacgt
    35341 cattgacccg ctgttctttc ctggcggtaa tatcggcaaa ctggcgattt gcggcaccgc
    35401 caatgacgtt gcggtcagtg gcgcaattcc gcgctatctc tcctgtggct ttatcctcga
    35461 agaaggattg ccgatggaga cactgaaagc cgtagtgacc agcatggcag aaaccgcccg
    35521 cgcggcaggc attgccatcg ttactggcga taccaaagtg gtacagcgcg gcgcggcaga
    35581 taaactgttt atcaacaccg ctggcatggg cgcaattccg gcgaatattc actggggcgc
    35641 acagacgcta accgcaggcg atgtattgct ggttagcggt acactcggcg accatggggc
    35701 gactatcctt aacctgcgtg agcaactagg gctggatgga gaactggtca gcgactgcgc
    35761 ggtgctgacg ccgcttattc agacgctgcg tgacattccc ggcgtgaaag cgctgcgtga
    35821 tgccacccgt ggtggtgtaa acgcggtggt tcatgagttc gcggcagcct gcggttgtgg
    35881 tattgaactt tcagaagcgg cactgccggt taaacctgct gtgcgcggcg tttgcgaact
    35941 gctgggactg gacgccctta actttgccaa cgaaggcaaa ctggtgatcg ccgttgaacg
    36001 caacgcagca gagcaagtgc tggcggcgtt acattcccat ccattgggaa aagatgcggc
    36061 gctgattggc gaagtagtag aacgtaaagg tgttcgtctt gccggtctgt atggcgtgaa
    36121 acgaaccctc gatctaccgc acgccgaacc gcttccgcgt atatgctaat aaaattctaa
    36181 atctcctata gttagtcaat gaccttttgc accgctttgc ggtgctttcc tggaagaaca
    36241 aaatgtcata tacaccgatg agtgatctcg gacagcaagg gttgttcgac atcactcgga
    36301 cactattgca gcagcccgat ctggcctcgc tgtgtgaggc tctttcgcaa ctggtaaagc
    36361 gttctgcgct cgccgacaac gcggctattg tgttgtggca agcgcagact caacgtgcgt
    36421 cttattacgc atcgcgtgaa aaagacaccc ccattaaata tgaagacgaa actgttctgg
    36481 cacacggtcc ggtacgcagc attttgtcgc gccctgatac gctgcattgc agttacgaag
    36541 aattttgtga aacctggccg cagctggcca caggtgggct ataccccaaa tttggtcact
    36601 attgcctgat gccactggcg gcggaagggc atatttttgg tggctgtgaa tttattcgtc
    36661 atgacgatcg cccctggagc gaaaaagagt tcaatcgtct gcaaacattt acgcagatcg
    36721 tttctgtcgt caccgaacaa atccagagcc gcgtcgttaa caatgtcgac tatgagttgt
    36781 tatgccggga acgcgataac ttccgcatcc tggtcgccat cactaacgcg gtgctttccc
    36841 gcctggatat ggacgaactg gtcagcgaag tcgccaaaga aatccactac tacttcgaca
    36901 ttgacgatat cagcatcgtc ttacgcagcc accgtaaaaa caaactcaac atctactcca
    36961 ctcactatct tgataaacag catcccgctc acgaacagag cgaagtcgat gaagccggaa
    37021 ccctcaccga acgcgtcttc aaaagtaaag agatgctgtt gattaatctc cacgagcggg
    37081 acgatttagc cccctatgaa cgcatgttgt tcaacacctg gggcaaccag attcaaacct
    37141 tgtgcctgtt accgctgatg tctggcgaca ccatgctggg cgtgctaaaa ctggcgcaat
    37201 gcgaagagaa agtctttacc actaccaatc tgagcttact gcgccagatt gccgaacgtg
    37261 tggcaatcgc tgtcgataac gccctcgcct atcaggaaat tcatcgtctg aaagaacggc
    37321 tggttgatga aaacctcgcc ctgaccgagc agctcaacaa tgttgatagt gaatttggcg
    37381 agattattgg ccgcagcgaa gccatgtaca gcgtacttaa acaagtggaa atggtggcgc
    37441 aaagtgacag taccgttctg atcctcggtg aaactggtac aggtaaagag ctgattgccc
    37501 gcgcgatcca taatctcagt gggcgtaata atcgccgtat ggtcaaaatg aactgcgcgg
    37561 cgatgcctgc cggattgctg gaaagcgatc tatttggtca tgagcgtggg gcttttaccg
    37621 gtgccagcgc tcagcgcatt ggtcgttttg aactggcgga taaaagctca ctattcctcg
    37681 atgaagtggg cgatatgccg ctggagttac agccgaagtt gctgcgtgta ttacaggaac
    37741 aggagtttga acgtctcggc agcaacaaaa tcattcagac ggacgtacgt ttaattgccg
    37801 cgactaaccg cgatctgaaa aaaatggtcg ccgaccgaga gttccgtagc gatctctatt
    37861 accgtctgaa cgtattcccg atccacctgc cgccactacg cgagcgtccg gaagatattc
    37921 cgctgctggc gaaagccttt accttcaaaa ttgcccgtcg tctggggcgc aatatcgaca
    37981 gcattcctgc cgagacgctg cgcatcctga gcaacatgga atggccgggc aacgtgcgtg
    38041 agctggaaaa cgtcattgag cgcgcggtat tgctaacacg cggcaacgtg ctgcagctgt
    38101 cattgccaga tattgcttta ccagaacctg aaacgccgcc tgccgcaacg gttgtcgccc
    38161 aggagggcga agatgaatat cagttgattg ttcgcgtgct gaaagaaaca aacggcgtgg
    38221 ttgccgggcc taaaggcgct gcgcaacgtc tggggctgaa acgcacgact ctgctgtcac
    38281 ggatgaagcg actgggaatt gataaatcgg cattgattta acagatgtgg ttatactcga
    38341 tcaacattca atgaaaggag gcagtatggc agtttcagca agaaatcaat taaccggaac
    38401 agttagcaca gtagcaatgg gcgctgtgaa tgatgaagta gaactgacgc tggcaggtgg
    38461 tgcaaagctg gtggcaattg tgactcacag cagccagcaa gcgctgggtc tggcgaaagg
    38521 gaaagaagcg attgccctga ttaaagcccc gtgggtcacg ctggcaacgg aagactgtgg
    38581 actgaaattc tctgctcgca accagtttgc tggcagcgtg tcgacaatca ccgaaggcgc
    38641 agtgaacgcc actgtccata tcaaaacgga tgcgggtttt gaaattgtgg cagtggtaac
    38701 aaatgaaagt caggatgaaa tgaaactcac cactggctcg cgcgttattg cattgatcaa
    38761 agcatcagcc atcctgattg ccaccaaagc gtaaacgcct tatttgccgg agagtatggc
    38821 tctccggcag atttatttct ttggacgata tttttctggc aactccggca ccggacgctt
    38881 gtcatcgatg agatgacgca cggttaagat cggatgacgc cacagcattc tcggcccggc
    38941 ccaacgcata atctgtttca tctcttcacg ctttgcaggc tggtaacagt gcaccggaca
    39001 ctgcttacag gctggtttct cttcaccgaa cacacattta tccagccgct tttgcgcgta
    39061 agcaaacaac gcctcgtaat gctccggctc tgctgacgcc tgcgggcatt tcgcttgata
    39121 aagatcgatc atttttttaa tcgtcagttt ttcacgagag atacgcttgc cggacatgct
    39181 gcctccatac tcttaaggtg catttatatt acaacttaat tttaaagggg actatggctc
    39241 tgaaggagga ggtttcttca accgacgaat ttggcgatct tgtttcatcg tcatcacgca
    39301 aaagctgcaa agcagcattt ttcgcggaac cgacatcaag aactcacctt tcgcgtcttc
    39361 ccctgaaatg attaactccg gtatcatgtg cgccttatgt gattacaacg aaaataaaaa
    39421 ccatcacact acatttaaca tcagggagcc ggacttaacc ccatgagaac aatagaaaat
    39481 ttcgacgccc atacgcctat gatgcagcag tatctcaagc tgaaagccca gcatcccgag
    39541 atcctgctgt tttaccggat gggcgacttt tatgaactgt tttatgacga cgcaaaacgg
    39601 gcgtcgcaac tgctggatat ttcactgacc aaacgcggtg cttcagcggg agagccaatc
    39661 ccgatggcgg ggattcccta ccatgcggtg gaaaactacc tcgccaaact ggtgaatcag
    39721 ggcgaatcgg ttgctatttg tgaacagatt ggcgatccgg cgaccagcaa agggccggtc
    39781 gagcgcaaag ttgtccgtat cgttacgccg ggcaccatca gcgatgaagc gctgttgcag
    39841 gaacgtcagg acaacctgct ggcggctatc tggcaggaca gcaaggggtt cggctacgcc
    39901 acgctggata tcagctccgg gcgttttcgc ctgagcgaac cggcggaccg cgaaacgatg
    39961 gcggcagaac tgcaacgtac gaatccagcg gaattactgt atgcggaaga tttcgccgag
    40021 atgtcgctga ttgaaggtcg ccgcggcctg cgtcgtcgcc cgctgtggga gtttgaaatc
    40081 gacaccgctc gccagcaatt gaacctgcaa tttggcaccc gagatctggt cggttttggt
    40141 gtggagaacg caccacgcgg actttgtgct gccggttgtc tgttgcagta tgcgaaagat
    40201 acccaacgca cgaccctgcc gcatattcgt tctatcacta tggaacgtca gcaggacagc
    40261 atcattatgg atgccgcgac gcgtcgtaac ctggaaatta ctcagaacct ggccggcggt
    40321 gcggaaaata cgctggcttc agtgctcgac tgtactgtaa cgccgatggg tagtcgtatg
    40381 ctgaaacgct ggctgcatat gccagtgcgc gatacccgcg tgttgcttga gcgccagcaa
    40441 actattggcg cattgcagga tttcaccgcc gagctgcagc cggtactgcg tcaggtcggc
    40501 gacctggaac gtattctggc gcgtctggct ttacgaactg ctcgtccacg cgatctggcc
    40561 cgtatgcgtc acgctttcca gcaactgccg gagctgcgcg cgcaactgga aggtgtcgat
    40621 agcgccccgg tacaagcact gcgtgagaag atgggcgagt ttgccgaact gcgcgacctg
    40681 ctggagcgag caatcatcga cacgccgccg gtgctggtac gcgacggtgg tgttattgcg
    40741 actggttata acgaagaact ggatgaatgg cgcgcgctgg ctgacggcgc gaccgattat
    40801 ctggagcgtc tggaagtccg cgagcgtgaa cgtactggcc tggacaccct gaaagtcggc
    40861 tttaacgcgg ttcatggtta ttacattcag atcagccgtg gacaaagcca tctggcaccc
    40921 atcaattaca tgcgtcgcca gacgctgaaa aacgccgagc gctacattat tccagagcta
    40981 aaagagtacg aagacaaagt tctcacttca aaaggcaaag cactggcact ggaaaaacag
    41041 ctttatgaag agttgttcga cctgctgttg ccgcatctgg aagcgttgca acagagcgcg
    41101 agcgcgctgg cggaactcga cgtgctggtg aacctggcgg aacgggccta tacgctgaac
    41161 tacacctgcc cgacctttat tgataaaccg ggtattcgca tcaccgaagg ccgccatccg
    41221 gtggttgaac aggtgctgaa tgagccgttt atcgccaacc cgctgaacct gtcgccgcag
    41281 cgtcgcatgt tgatcattac cggtccgaat atgggcggta aaagtaccta tatgcgccag
    41341 accgcactga ttgcgctgat ggcctacatc ggcagctatg taccggcaca aaaagtcgag
    41401 attggaccta tcgatcgcat ctttacccgc gtaggggcag cggatgatct ggcatccggg
    41461 cgttcaacct ttatggtgga aatgaccgaa accgccaata ttttacataa cgccaccgag
    41521 tacagtctgg tgctgatgga tgagattggg cgtggaacgt ccacttacga tggtctgtcg
    41581 ctggcgtggg cgtgcgcgga aaatctggcg aataagatta aagcgttgac gttgtttgct
    41641 acccactatt tcgagctgac ccagttaccg gagaaaatgg aaggcgtcgc caacgtgcat
    41701 ctcgatgcac tggagcacgg cgacaccatt gcctttatgc acagcgtgca ggatggcgca
    41761 gcgagcaaaa gctacggcct ggcggttgca gctctggccg gcgtgccaaa agaggttatt
    41821 aagcgcgcac ggcaaaaact gcgtgagctg gaaagcattt cgccgaacgc cgccgctacg
    41881 caagtggatg gtacgcaaat gtctttgctg tcagtgccag aagaaacgtc gcctgcggtc
    41941 gaagcactgg aaaatctcga cccggattca ctgactccgc gtcaggcgct ggagtggatt
    42001 tatcgcttga agagtctggt gtaataacaa ttcccgatag tcttttgcta tcgggaatat
    42061 taacgacaac tgacgaatca aataaaaata ccctgtataa taggaaatct tattttacag
    42121 ggtaaaacca tgccatctac acgctaccaa aaaatcaatg cccatcacta tcaccatata
    42181 tgggccgttg gtgatattca tggtgactat caattattac aatcccgctt acatcaactc
    42241 tctttttgtc ctgaaaccga cttgcttatt tctgtcggcg ataacatcga tcgtgggccc
    42301 gagagtctta acgtgctgcg cctgttaaac cagccctggt ttatctccgt caaaggcaac
    42361 cacgaagcga tggcgcttga ggcattcgaa actggcgatg gaaatatgtg gcttgccagc
    42421 ggtggtgact ggtttttcga tttaaatgat tcagagcaac aagaggcaat agatctgttg
    42481 ctgaaattcc atcaccttcc acatattatt gaaatcacta acgacaccat aaaatatgtc
    42541 atcgcacatg cagattatcc ggggaaggag tatcagtttg gcaaagaaat agcggagagc
    42601 gaattactct ggcctgttga tcgtgtgcag aaatcactta atagcgaatt acaaaaaata
    42661 aacggcgctg attattttat atttggacat atgatgtttg ataacattca gacgttcgca
    42721 aaccagattt atattgatac cggatcgccg aaaagcgggc ggctgtcatt ttataaaata
    42781 aagtagtctc atgcttcttc tgtgaagcat gagtaacccg gcgttattgc aggtcattat
    42841 tcatttttcg ctaccagcaa agagagatcc agcttcgcca gcgcgcggct ggcactctcc
    42901 ggcagaccat cgtcagtaat gatctgatca aactcgctta atggtaacgc cagccatgtc
    42961 gccacctgtc catatttcgt cgcatcacag accaaaactc gctggcggct ggcactggca
    43021 atcgcccgtt tcaccgtgac tttatcttct gctggcgtag agatcccccg cacactccat
    43081 gacgatgcag aaataaaagc ctgatcaatc atcaggctac gcagcatggt cgcagcggct
    43141 tccccaacac aggaacggtt ttcccgacac actgcgccgc cagtgtgaat aattgtgcaa
    43201 ttactgttgt cgagcaagta gtccgcaata acgaagtcgt tagtaaccac tgtgagtggc
    43261 tccatgtgag tcagatgctg tgcaatcgct aacgtggtcg ttcccgcatc cagatagata
    43321 caacgtcccg gctgaacaag acttgccgcc agcttgccaa tagccgcttt ttgcgtcatt
    43381 gccagcgcag tttttacctg atgagaaggt tcatgcgcca cccgtcccgg agactggacg
    43441 cccccggaca ccaacacaac ggctccctgc tgctccagtt tttgtaaatc ccgacgaatg
    43501 gtcatatgtg acacattcat tctgtccgtt agttcagcaa tactaacaat gcctttttca
    43561 gctaccatct caaggatgat ttggcgacgc tctacgggta tcaacttttg ctccttcctt
    43621 tgtcctgctg acattctacg ctatttgcct gcgaaacgtg cgcggcgcaa ctaacgctta
    43681 gttcacataa aataacatac aatgttaatt tttgtgaatc agctcaccat atcgttatct
    43741 ttccgcgctt atattcacaa catcaaacaa aatatcactt aaattaacaa ggagagcaga
    43801 tgaaaacggg atcggagttt catgtcggta tcgttggctt agggtcaatg ggaatgggag
    43861 cagcactgtc atgtgtccgc gcaggtcttt ctacctgggg cgcagacctg aacagcaatg
    43921 cctgcgctac gttgaaagag gcaggtgctt gcggagtttc tgataacgcc gcgacgtttg
    43981 ccgaaaaact ggacgctctg ctggtgctgg tggtcaatgc ggcccaggtt aaacaggtgc
    44041 tgtttggtga aacaggcgtt gcacaacatc tgaaacccgg tacggcagta atggtttctt
    44101 ccactatcgc cagtgctgat gcgcaagaaa ttgctaccgc tctggctgga ttcgatctgg
    44161 aaatgctgga tgcgccagtt tctggtggtg cagtaaaagc cgctaacggt gaaatgactg
    44221 tcatggcctc cggtagcgat attgcctttg aacgactagc acccgtgctg gaagccgttg
    44281 ccggaaaagt ttatcgcata ggtgcagaac cgggactagg ttcgaccgta aaaattattc
    44341 accagttgtt agcgggcgta catattgctg ccggagccga agcgatggca cttgcagccc
    44401 gtgcggggat cccgctggat gtgatgtatg acgtcgtgac caatgccgcc ggaaattcat
    44461 ggatgttcga aaaccggatg cgtcatgtgg tggatggcga ttacaccccg cattcagccg
    44521 tcgatatttt tgttaaggat cttggtctgg ttgccgatac cgccaaagcc ctgcacttcc
    44581 cgctgccatt ggcctcaaca gcattgaata tgttcaccag cgccagtaac gcgggttacg
    44641 ggaaagaaga cgatagcgca gttatcaaga ttttctctgg catcacccta ccgggagcga
    44701 aatcatgatc aagattggcg ttatcgccga tgattttacc ggcgcgacgg atatcgccag
    44761 ttttctggtg gaaaacggtc taccaacggt acaaattaac ggtgttccaa caggtaaaat
    44821 gccggaagca atcgacgcac tggtgatcag cctgaaaacg cgctcctgtc cggtggttga
    44881 agccacacag caatcgctgg cggctctgag ctggttgcaa cagcaaggtt gcaaacagat
    44941 ctatttcaaa tactgctcta ctttcgacag ttcggcgaaa ggtaatatcg gcccggttac
    45001 cgatgcatta atggatgctc tcgacacgcc gtttacggtc ttctctccgg ccctgccggt
    45061 caacggacgt acggtttatc aggggtattt gttcgtaatg aatcaactgc tggccgaatc
    45121 cgggatgcgc catcacccgg taaatcccat gaccgacagc tatcttcccc gtctggttga
    45181 agcgcaatcc acagggcgct gcggcgtcgt ttcggcacat gttttcgaac aaggtgtgga
    45241 tgccgttcgt caggagctgg ctcgcttaca gcaagagggc taccgctacg cggtgcttga
    45301 tgcgctgacc gaacaccatc tggaaattca gggagaagcc ttgcgcgata ccccactggt
    45361 aacgggcggt tctggtctgg cgattggcct tgcccggcag tgggcgcaag aaaacggtaa
    45421 ccaggctcgc gaagcagggc gtccgctcgc tgggcgcggc gtagtgctct ccggttcatg
    45481 ctctcaaatg accaaccgcc aggtggcaca ttaccgtcaa attgcaccag cccgtgaggt
    45541 tgatgtggca cgctgcctct caaccgaaac tctggccgct tatgcacacg agctggcaga
    45601 gtgggttctg ggccaggaaa gtgtacttgc tccactggtt tttgccaccg ccagcactga
    45661 cgcattggca gcaattcaac agcaatacgg tgcacaaaaa gccagtcagg cagtggaaac
    45721 actgttttct caactagcgg cgcggttagc agcggaaggc gtgacacgct ttattgtcgc
    45781 aggcggtgag acctccggcg tagtcacaca gagcctgggc ataaaaggtt ttcatattgg
    45841 cccaaccatt tcccccggcg tgccgtgggt aaacgcactg gataagcctg tctcactcgc
    45901 ccttaaatct ggcaacttcg gtgatgaagc ctttttttca cgagcccaaa gagagttttt
    45961 atcatgagcg atttcgcaaa agtagagcag tctttgcgag aggagatgac gcggattgcc
    46021 agttcattct ttcagcgcgg atatgcaact ggttcggctg gcaatctgtc gctgctttta
    46081 cctgacggga atttactggc gacaccgaca ggttcatgcc tgggcaatct cgatccgcag
    46141 cggctttcca aagtcaccgc ggatggcgaa tggttaagtg gtgacaaacc ctcgaaagag
    46201 gtgctctttc atctggcgct gtatcgcaac aatccgcgct gtaaagcggt agtgcatttg
    46261 cacagtacgt ggtcgacggc gctttcctgc ctggaagggc tggacagcaa taacgttatt
    46321 cggccgttca caccatacgt ggtgatgcgg atgggaaacg tcccgctggt gccctattac
    46381 cgaccgggcg ataaacgcat cgcacaggat ctggcggaac tggcagcaga caatcaggct
    46441 tttttactgg caaatcatgg cccagtggtt tgcggtgaaa gcctgcaaga agccgccaac
    46501 aatatggaag agctagagga aacggcaaag ctgattttta ttctcggtga ccgcccgatc
    46561 cgttatctga ccgcaggtga aattgcggaa ttaaggagtt aatgcaatgc ctcgttttgc
    46621 agctaattta tccatgatgt tcaccgaagt gccttttatt gaacgcttcg ccgcagcgcg
    46681 aaaagccggg ttcgatgctg tggagtttct gtttccctat gactactccc cccagcaaat
    46741 ccagcagcaa ctggcgcaaa accacctgac gctggcgctg ttcaataccg ctcccggaga
    46801 tattaatgcg ggagaatggg gattatccgc ccttcccgga cgtgagcacg aagcacgtgc
    46861 agacatcgac ctggcactgg aatatgcatt ggcgctcaat tgtgaacaag tccatgtgat
    46921 ggcaggcgtc gtgcccgctg gcgaagatgc tgaacggtac cgggcagtat ttattgataa
    46981 tctccgctac gctgccgacc actttgcgcc acacggcaag cgaatattgg ttgaagcact
    47041 cagccccggc gttaagcctc attatctctt ctccagccag tatcaggcac tggctattgt
    47101 tgaagaggtt gcgcgagata acgtttttat tcaactagat actttccatg cgcaaaaagt
    47161 agatggcaac ctgacgcatt taatccgcga ctacgccggg aaatatgcgc acgtacaaat
    47221 tgccggacta cctgatcgac atgaaccgga cgatggagaa atcaactatc cgtggctgtt
    47281 ccgcctgttc gatgaagtgg gatatcaggg ctggatcggt tgtgaataca aacctcgtgg
    47341 cctcaccgaa gaaggacttg gctggtttga cgcctggcgc taattcgtta aatccctaat
    47401 tacaacgtac ccatacatcc ccccatctac ggatggggta ggttaactat aactttcaga
    47461 cagggtttcc catgtccaca attacattgt tatgcattgc gttaactggc gtaatcatgc
    47521 tgttgctgct ggtcatcaag gcaaaggtac aaccattcgt tgctctgctc ctcgtcagcc
    47581 tgttagtcgc acttgcggcg ggtataccgg cgggcgaagt gggtaaagtg atgatcgccg
    47641 ggatgggcgg tgtgcttggt tccgtcacta ttattattgg tctgggcgct atgctggggc
    47701 gtatgatcga acactctggt ggtgcagagt cactggctaa ttatttcagt cgcaagttag
    47761 gcgacaaacg aactatcgct gcgctgactc tggcagcgtt cttcctcggt attcccgtct
    47821 tctttgatgt cggctttatt attcttgcgc caatcattta cggttttgcc aaggttgcca
    47881 aaatatcacc actcaaattt ggtctgcctg tcgctgggat catgctcact gttcacgtgg
    47941 cggtaccgcc tcatccaggc cctgtcgccg cagcggggtt actccacgca gacatcggct
    48001 ggctaaccat catcggtatt gcgatttcta ttcccgtagg gattgttggc tactttgcag
    48061 cgaaaataat caataagcgc caatatgcga tgtcggtcga ggtactggaa cagatgcaac
    48121 tggctccggc cagtgaggaa ggcgcgacaa aattaagcga taaaataaat ccaccgggcg
    48181 tcgcgctggt cacctcgcta attgttattc ctatcgcgat tatcatggcg ggtacggttt
    48241 ccgcaacact gatgccgcct tcgcatcccc tgcttggtac gctacagctg atcggctcac
    48301 caatggtagc tctaatgatt gcgctggtgc tggcattctg gttattggct ttacgtcgcg
    48361 gctggagctt acaacacacc agcgacatta tgggctcagc ccttcccact gcggcggtag
    48421 tgattttggt tactggtgct ggaggggtat ttggcaaagt gctggtggaa tcgggcgtcg
    48481 gtaaagccct tgccaacatg ttacaaatga ttgacctgcc tctgttacca gccgcattta
    48541 ttatttcact ggcgctgcgt gcatcgcagg ggtcagcaac cgtagcaatc ctgacaaccg
    48601 gcgggttact ctcagaagcg gtgatgggat tgaatccaat tcagtgcgta ttggtgacgc
    48661 tggcagcctg ctttggtggg cttggcgcgt cacatattaa tgactcaggg ttctggattg
    48721 tgaccaaata tctggggttg tcggtagcag acgggctgaa aacctggaca gtgttaacga
    48781 ccattctcgg ttttaccgga ttcttaatta cctggtgcat atgggcggta atttgacctt
    48841 aataaaaggt ccgatgggca tcggaccttt tatttagcgc ttaccttccg ccagcagcgg
    48901 cgggatgctc ggcaccatcg gcgcgtcatc aatatctttc tgcgtcatgc ggaacgcttc
    48961 gggataatgt tcgcggctgg tgcggcgtag cggttcggta tcgcgccagg tatagaggca
    49021 atgctggcac tggtagacca tccagacatc ttttaccggc gatttcgcca tcacttcaat
    49081 ctgttcatcg gcacaacgtg gacaaatcat cttgttctcc ttattgacgt gcggccagca
    49141 tagcggtcag tttttcagcc caggctttgg tttccggtaa gtccaccacc ggctggctgt
    49201 agtgaccacg gttgtccggg gcgacaggcg tggtggcgtc gataatcagc ttgtcggtga
    49261 tccccgccgg acttgagcca gggtcgagtt ccagcacgga catattcggc aactgtacca
    49321 aatcccctgc cgggttcact ttcgaggaga gcgcccacat cacctgcggc aggttgaacg
    49381 gatcaacgtc ttcatcgacc ataatcacca tcttcacgta gcccagaccg tgcggcgtgg
    49441 tcatcgcacg caggcccacc gcgcgggcaa agccgccgta gcgttttttg gtggagataa
    49501 tcgccagcag gccgtgggtg tacatggcgt ttaccgcctg cacttccggg aactcggctt
    49561 tcagttgttg atacagcggc acgcaggtgg caggccccat caggtagtcg atttcggtcc
    49621 acggcatacc aagatagagc gattcgaaaa tcggtttggt acggtaagag actttatcga
    49681 tacgcaccac ggtcatgtta cgcccgccgg agtagtggcc ggtaaactca ccgaacggcc
    49741 cttcaatctc acgtttacga ctttcgataa ccccttcgag gatcacttcc gagccccacg
    49801 gcacatcaaa accggtcagc ggggcggtgg cgatcgggta cgggctttcg cgcagcgcgc
    49861 ctgccatttc gtactcagac tgatcgtatt tcagcggcgt ggcccccatt agggtgatga
    49921 tcggatcgtt accgagtgtg atggcaatcg gcagatcttc accgcgctct tctgctttat
    49981 gcagatgcag ggcgatatcg tgcatcggca ccggttgcag gccgagctta cgcttgccct
    50041 tcacttccat gcggtagatg ccgacgttct gcttgccgaa gttatccggg tcgagcggat
    50101 cgcgggaaac cacgcacgct ttatcgagat agaaaccgcc atcaccgtcg tttaaacgaa
    50161 acagcggcag aatattgaac aggttgatct cgtcgccatc gacggtgttc tgcgcccagg
    50221 ctggattggc gcggcgctcc ggggcaatgg ggaagttatc ccagcggcgg ataaactcat
    50281 caatctgttt tttaaccggg gtgtttggcg gcaggccgag ggaaatcgcg tggttctgcc
    50341 aggaaccgat ggtgttcatt gccacgcggg catcgataaa gccgcgaata ttatcaaacc
    50401 aaagcgcggg cgcgccgtcg ccgatacgcc cggtggcgtt ggctgcggct gccagatccg
    50461 gttccgcgtt cacctcttcg ctgattttca gtaactggcc gtggtcatca agcgcctgta
    50521 aaaagctgcg taaatcatca aatgccatta ttcattctcc tgagaaaaat tccgggcctg
    50581 cggcaatcct tgccagcgcc tggcgtgagg atgttcgagg ccaaattgat ccagcacgcg
    50641 ggctaccacg tggtggacaa tgtcatctac cgtttcggga tggttataaa aggcaggcat
    50701 cggcggcacc atcgccacgc ccatgcgcga aagtgcgagc atattttcga gatggattgt
    50761 gctaagcggc atttcacgcg gcaccagcac cagtttgcgg ccttctttga gcacgacgtc
    50821 cgccgcgcgc cccaccaggc catcggcgta accagcgcgg ataccggcga gcgttttcat
    50881 actgcatgga ataacgatca tgccgtcggt acgaaaggag ccggaagaga tagtcgccgc
    50941 ctgatccgcc gggttatggc tgaagtctgc gagggcggca acatcgcgag cgctgtaagg
    51001 cgtttccagt tcaatcgtgg ttttcgccca cttcgacatc actagatgag tctcgacatt
    51061 cggcatctcc cgcagcgctt gcagtaatgc cacaccaaga ggcgcaccgg tagcccctgt
    51121 catcccgacg atcagtttca ttgcttactc tcttaattgt tcgtatacga acgttattat
    51181 taacatactc cttcatttcc acagcggcaa ggattgaaaa gtaacaacat cttcttcgtc
    51241 actgaaaaac cgctatgata gcgacagaga attagcccgg agtttacatg gcgttacgaa
    51301 ataaagcgtt ccatcagtta cgacagcttt ttcagcagca tacagctcgc tggcagcacg
    51361 agttacctga cctgaccaaa ccacagtacg cggtgatgcg cgccattgct gataagcctg
    51421 gtatcgaaca ggtggcactg atagaagcag cagtcagcac caaagcaacg ctggcagaaa
    51481 tgttggcaag aatggaaaat cgtggcttag tcagacgtga acatgacgcc gctgacaagc
    51541 ggcgacgctt tgtctggctg actgccgaag gagaaaaggt acttgctgcg gcgatcccca
    51601 ttggtgacag tgtggatgag gaatttttgg ggcgtttgag tgcagaagag caggagctgt
    51661 ttatgcagtt ggtgcgcaag atgatgagca aataggatgc tgattgccgg gtaggacgct
    51721 gacgagtctt atccaggcga caaaacacac cgacttccca aacaaaagaa aaaggccagc
    51781 ctcgcttgag actggccttt ctgacagatg cttacttatt cgcggaacag cgcttcgata
    51841 ttcagcccct gcgtttgcag gatttcgcgc aaacggcgca ggccttcaac ctgaatctgg
    51901 cgaacacgtt cacgggtgag gccaatttca cgacctacat cttccagtgt tgccgcttcg
    51961 taccccagca aaccgaatcg acgtgccagt acttcacgct gtttggcgtt cagctcgaac
    52021 agccatttga cgatgctctg cttcatatcg tcatcttgcg tggtatcttc cggaccgttc
    52081 tctttttcat cggccaggat gtccagcaac gctttttcgg aatcaccacc cagcggggtg
    52141 tctaccgagg taatgcgctc gttaagacga agcatacggc tgacgtcatc aactggctta
    52201 tccagttgct ctgcgatctc ttccgcactt ggttcgtggt ccagtttatg ggacaactcg
    52261 cgtgcggttc gcaggtaaac gttcagctcc tttacgatgt gaataggcaa acgaatagta
    52321 cgggtttggt tcataatcgc ccgttcgatc gtctggcgaa tccaccaggt tgcgtatgtt
    52381 gagaagcgga aaccacgttc cgggtcaaac ttctctaccg cgcggatcag ccccaggttg
    52441 ccctcttcga tcaggtccag caacgccaga ccacgattgc cataacggcg ggcaattttt
    52501 accaccagac gcaagttact ctcgatcatc cggcggcggg aggcaacatc tccacgcagt
    52561 gcgcgacgcg caaaataaac ttcttcttcg gccgttaaca gtggtgaata accaatctca
    52621 ccaaggtaaa gctgagtcgc gtccagcaca cgctgtgtgg ctccctgcga taacagttcc
    52681 tcttcggcca aatcgttatc actgggttcc tcttctacta aggccttttc gtcaaaaacc
    52741 tcaactccgt tctcatcaaa ttccgcatct tcatttaaat catgaacttt cagcgtattc
    52801 tgactcataa ggtggctcct acccgtgatc ccttgacgga acattcaagc aaaagcctgg
    52861 ttccgccgat ttatcgctgc ggcaaataac gcagcgggtt tacggatttc cccttgtaac
    52921 gaatttcaaa atgcaagcgt gttgaactgg ttccggtgct acccatggtt gctatttttt
    52981 gccccgcctt cacttcttgt tgttcccgga ccagcattgt gtcgttatgg gcgtaggcac
    53041 tcaggtaatc atcattatgt ttgatgataa tcagattacc gtagccgcgc agcgcgttac
    53101 cggcataaac aacgcggcca tctgcggtcg cgataattgc ctgtcctttg ctgcctgcga
    53161 tatcaatccc cttgttgccc ccctcagaag cgccaaaggt ttcgatcact ttgccctcag
    53221 tcggccagcg ccaggtggag ataggcgtac tggttgatgt actgctgaca gtcggctcgg
    53281 ttgtgcttgc tgttggtacc gttacaggcg ctgtgaccgt ggtcgcagtt ggcttgttgt
    53341 tcggcaacat tttgttagca ctctgttcac ccgaagactc agaatacgta attgtcggtt
    53401 gcgacgcaac agcaacggtg gaattttgtg caggcttgat cacaactcct tgctctgctg
    53461 cgtcggcctg ggtaatggca tttccgccag tgattggcgt accggaagca ttacccacct
    53521 gtaaggtctg accgacgttc agcgcgtatg gtgcctgaat attgttgcgc tgagcaaggt
    53581 cacggaaatc gttgccagta atccaggcga tatagaaaag tgtgtcgcct tttttcacgg
    53641 tataggtact gccgctataa ctgcctttcg gaatgttccc atactgacgg ttgtagacga
    53701 tgcgtccgtt ttccatctgt accggctgct gagctactgg ctgtactggc tggatttgcg
    53761 gttgttgagt agcctgaatt tgtggctgct gtaccggctg aatttgcggt tgctgcgctg
    53821 tagacgtcgt ccccattttc ggcggcggcg taatcaacat accagaattg gtatttgcag
    53881 gcgcattgcc attaacggag ctgaccggtg ccggtggatt tgaagtgtca gaacagcctg
    53941 ccagccatag cgaaaccagt gacaaagccg caatgcggcg aacggtgaat tttgggcttc
    54001 ccgcgctcat ttatccccca ggaaaaattg gttaataacc agtgacataa ttaccgtgca
    54061 aggcaccata ctgaacactg gaaaagatgt tcacgatacg ctgacctgcg gcaaaataac
    54121 caggaaaaat ccaggtattt cctcacgttt taagccagct cacccttcac taaagggaca
    54181 aagcgcacgg cctccacggt atcgataata aattcgcctc cccgacgacg cacccgtttc
    54241 aaatactggt gctcctcccc tacgggtaag acgagaattc cgccttcgtc cagctgcgtc
    54301 attagcgcag ttggaatttc cggcggtgcc gccgtaacaa tgatagcgtc aaacggcgca
    54361 cgtgcctgcc aaccttgcca tccatcgcca tgacgggttg aaacattatg taaatcaaga
    54421 tttttcaggc ggcgacgcgc ctgccactgc aagcctttaa tccgttcaac cgagcaaaca
    54481 tgctggacaa gatgcgccag gattgccgtt tgatatcccg aaccggtgcc aatttccagc
    54541 acccgcgact gcggcgtcag ttcgagtaat tcggtcattc gcgccaccat atacggctgc
    54601 gaaattgtct gcccctgacc tatcggcaag gcgatattgt cccaggcttt ttgttcaaac
    54661 gcttcatcaa cgaatttttc acgcggcacg gcggcaagtg cattcagcac cagctcatcc
    54721 tgaatacctt gcgcacgtaa ttgatccaga agtgcttgta cgcgtctgct taccattgcg
    54781 tgccaactcc cacgctgttt aaccagtctg aaaccacatc ttgcgcgcta tgcgcagtta
    54841 aatccacatg cagcggcgtg atggaaacat agccctcatc taccgcagca aaatcggtcc
    54901 ccggaccggc atcacattta ccgcccggcg ggccaatcca gtacagcgta ttgccgcgcg
    54961 gatcttgctg cgggatcacc tgatctgccg gatgtcgtgt accgcagcgc gtcacgcgaa
    55021 tacctttgat ttgatccaat ggtaaatccg gaacgttaat attaagaata cgtccggtgc
    55081 gcagcggctc tttacacagt gcgcgcaaaa ttgaacaggt taccgccgcg gcagtgtcgt
    55141 aatgtttatg cccgtcaagc gagacggcca gcgccggaaa acctaaatga cggccttcca
    55201 tcgcggcggc taccgtaccg gaataaataa catcatcccc cagattcggc ccggcgttaa
    55261 ttccggacac aacaatgtcc gggcgcggac gcatcagagc attcacgcca agatagacgc
    55321 aatcggtcgg ggttcccatt tgcacagcaa tatcaccatt ttcaaaggta aacgtgcgca
    55381 gggaggattc cagtgtcaga gaatttgaag cgccgctgcg gttacgatcg ggggcgacca
    55441 cctgaacgtc agcaaactca cgcaaggctt tcgccagcgt ttgtataccg ggtgcatgta
    55501 ccccgtcatc attactcagc aatatgcgca taatcacctg ttgtgttgat aagttccctg
    55561 acaacgctgg ttgcaaaact tcccgccgga agccagaaac ggatttctac ggtgacgtca
    55621 tcccaccagt tccagcttaa tttttgcgga tacagcagca tcgctctgcg cgcggcttca
    55681 actttttcac gcaccagtaa agtttgtaat tcagtttctt cggcgacagc tgcttgttcg
    55741 aatgccagcg cttcacgctg agttccccat tcgccactgc ccggcaatgc ggcggttatc
    55801 atcagctctt tatcgttgac gcgacgctgt aattccgcca gttcttcggt ggttgcaaca
    55861 aaccagctac cacgtccggc taattgtagc gcatcgccgt caacaacttg attaacgtct
    55921 gcttttttga ggcgctcagc aacaatctga ttaaacaacg cactgcgggc tgccgacaac
    55981 caaaaactcc gtttattgcg atcgcgcacc ggagtattgg tttgcgccca acgcagcgcg
    56041 ccctgcaagt tgctaccgcc aatcccaaaa cgttgggcac cgaagtagtt cggtacacct
    56101 tttacgcaaa tatcgatcag acgttgttca acgtcatcgc gattgctcac ttcgcgtagt
    56161 accagggtaa aggcgttacc tttcagcgcc cctaaacgca gcttgcgctt gtgccgcgca
    56221 tactccagca cctggcagcc ttccagttga aaggcgctca gatcgggcat ctctttgccc
    56281 ggcacgcgag cgcataacca ctgttccgta acagcatgtt tatctttttg cccagcgaag
    56341 ctgacttcac gggcatgaat tttcaggaat ttcgccagtg catccgccac aaaacgggta
    56401 ttgcagccgt ttttgaggat tctaaccaga atatgctcac cttcaccatc aggctcaaag
    56461 cccaaatctt ccaccaccac aaagtcttcc ggattggctt tcagcagccc ggtgccttgc
    56521 ggtttaccgt ggaggtaagt gagattatca aactcaatca ttttgttgcc ttaatgagta
    56581 gcgccaccgc ttcacaggca atcccttccc cacgtccggt aaatcccagt ttttccgtag
    56641 tagtggcttt cacgttaaca tcatccatat ggcagccgag atcttcggca ataaacacgc
    56701 gcatttgtgg aatgtgcggc aacatcttcg gtgcctgagc gatgatagtg acatcgacgt
    56761 tgccgagggc ataacctttc gcctgaatac gacgccaggc ttcgcgcaac agctcgcggc
    56821 tgtcggcacc tttaaatgcc ggatcggtat ccgggaacag cttgcctata tctcccagcg
    56881 ccgccgcgcc aagcaatgca tcggtcaacg catggagcgc cacgtcgcca tcagaatgcg
    56941 ccagcagccc tttttcgtaa ggaatgcgta cgccaccaat gataattggg ccttcaccgc
    57001 caaaggcgtg tacgtcaaaa ccgtgtccaa ttcgcattat gtattctcct gatggatggt
    57061 tcgggtgagg taaaattcgg ccagcgccaa atcctccggg cgcgttacct tgatgttatc
    57121 cgcgcggcct tcaacaagtt gcggatgaaa accgcaatat tccagcgccg aggcctcatc
    57181 ggtaatggta gcgccttcat ttagagcgcg cgtcaggcag tcatgtaaca gctcacgagg
    57241 gaaaaattgc ggcgtcagcg cgtgccataa gccgttgcga tcaacggtat gagcaatggc
    57301 atttttgccc ggttcggcac gtttcatcgt atcgcgcacc ggcgcagcga ggatcccccc
    57361 cgtgcggctg gtttcgctca acgccaacaa tcgcgcgagg tcatcctgat gcagacaagg
    57421 acgagcggcg tcatgcacca atacccactg cgcgtcgcca gcggctttca gacctgccag
    57481 cacggaatcg gcacgctctt caccaccatc aacaaccgtg attcgtggat gattcgccag
    57541 aggaagttgt gcaaagcggc tatcgccagg acttatggca atgacgacac gtttcaccct
    57601 gggatgcgcc agcagcgcat acaccgagtg ttcaagaatg gtttgattac cgattgagag
    57661 atattgctta ggacattccg tttgcattcg gcggccaaat ccggccgccg gaaccacggc
    57721 gcaaacatcc aaatgagtgg ttgccatgtt aattcccggg ctgatttatc gattattttg
    57781 ccccgcagac tgtgcgcgct ttgacgcgtc aggcaccaga cgataaaacg tttcgcccgg
    57841 cctggtcatg ctgagttcat tacgcgcacg ctcttcgagc gcctcctggc cgccattgag
    57901 atcgtcaatt tcggcaaaaa gctgatcgtt tcgcgcttta agtttcgcgt ttgtagcttg
    57961 ctgtgccgcc acatcattat tgacgcgagt atagtcatgt ataccgttct taccgaacca
    58021 cagcgaatac tgtagccaga ccagaatagc cagcaacagc agcgttagtt tacccatcct
    58081 gccccctgaa aaacggcatc ttcatcccat gcatccgaag acgactctac atcctctgtt
    58141 ggggataccg cgacaacgcg ggcaaatgta ccacatttgt ccattgttac gtatacccag
    58201 ggcgtgcaga acataatctc attattagtt acggattgaa ttatgaacag aatagagagg
    58261 aaagaacaat taacccagta gccacataaa cagcgcacca aacataatgc caaccgtcgc
    58321 cagagtgaaa acaatactgt agcgtagctt tccgtccatc aatgaatgca gtgcaatccc
    58381 caccactaca gcgacgggca tcagcgccag aaagaaaggc caggtgtaga taaaaaagag
    58441 cagcgtgtta gagccataaa tcaacatcgg aattgccaac gcgcacagcc acgataaaaa
    58501 accaaccaca gcgcctggca gcgaccatgt ggtgtcttca acctcagtaa ggctgtcgtt
    58561 atttgttaat gtaatgttgt ggctgttacg catatctgat cctgttactt tgacgaaccg
    58621 ggcatggaaa cccggcggtg tctcaggatc tgataatatc gttctgtctc aacagatcta
    58681 ataattgctg taccaaattt gttactaatt gttcaccatt gagatgaatt tctgccgatt
    58741 caggcgcttc gtaaacggaa tctattcccg taaagttgcg cagttcaccg gcacgcgctt
    58801 tcttatataa accttttgga tcgcgggctt cgcaaatcgc cagcggcgta tcgacaaaca
    58861 cttcgataaa gcgcccttct cctacgcgtt cgcgaaccat ctggcgttcg gcgcggtgtg
    58921 gcgagataaa tgcggtcagc accaccagtc cggcttcaac catcaaattc gccacttcac
    58981 caacccgacg gatattctct ttacggtcgg catcgctaaa accgagatcg ctgcataatc
    59041 cgtggcgaac gttgtcgcca tccagcagat acgtactgac gccaagttta tgtaacgcct
    59101 cctccagcgc ccccgcgacg gttgatttac cggaaccaga gaggccggta aaccacagca
    59161 caacaccacg atgaccgtgg tgctgttcgc gttgttgcgg agttactggg tggctatgcc
    59221 agacgacgtt ttcgtcatgc agcgccatta tttatctccc agtaaatcgc gcgcacccca
    59281 atgcgggaaa tggcggcgaa ccagggcatt caattccagt tcgaatgcac tgaattcaga
    59341 tggcgcagca gttgcctggc taactggttc gtgcaccata ccggcaccta cggtcacatt
    59401 actcaggcga tcgataaaaa tcagcccacc ggtaaccggg ttttgctgat aacgatctaa
    59461 caccagtggc tcgtcaaagg tgagatctac caggccgatg ccgttcagcg gcaggttttc
    59521 aacttcgcgt tgggtaaggt tattgatatc aacctgataa cgaatgccat caacacgagc
    59581 acgcgtcttc ttaccggcaa ttttgatgtc ataactctgg cccggggaaa gcggctgttc
    59641 cgccatccat accacatcca ccgacgcgct ctgcacagcc ggtaacgctt cgtctgccgc
    59701 cagcagcaga tcgccacggc tgatgtcgat ctcatccgtc agcaccaggg tgatcgcttc
    59761 tccggcaaag gcttcttcgc gatcgccatc aaaagtcacg atccgcgcga cgtttgattc
    59821 cacaccagag ggcagcactt tgacacgttg cccgacttcc acgcgaccgg atgccagcgt
    59881 tccggcgtag ccacgaaaat cgagattcgg acggttaacg tactgcaccg ggaagcgcat
    59941 tggctgagca tccaccactc gctggatctc cacggtttcc agcacttcta gcagtgtcgg
    60001 accgctgtac cacggcatac tttcactttg cgaagccacg ttgtcgcctt ccagtgcgga
    60061 gagcggcaca aagcggatat ccagattacc cggcagctgc ccggcaaaag tcagataatc
    60121 ttcacgaata cgggtgaacg tctcttcact gtaatccacc agatccattt tgttgatcgc
    60181 cacgaccaga tgtttgatcc ccaacagtgt ggagataaaa ctatgacgac gggtttgatc
    60241 gagcacgcct ttacgggcat cgatcagtaa gatcgccagt tcacatgtcg atgcgccagt
    60301 cgccatattg cgggtgtact gctcgtgccc tggggtgtcg gcgataataa atttacgctt
    60361 ctcggtagag aaatagcggt aggccacatc aatagtgatg ccctgttcgc gctcagcttg
    60421 caggccgtcc accagcagtg ccagatccag cttttcgccc tgggtgccgt gacgcttact
    60481 gtcgttatgc agcgatgaga gctgatcttc gtagatttgg cgggtatcgt gcagcaggcg
    60541 accaatcagg gtacttttgc cgtcatcgac gctaccacag gtcagaaaac gcagcaggct
    60601 tttatgttgt tgcgcaatca tccatgcttc gacgccgcct tcattggcga tttgttgtgc
    60661 aagtgcggtg ttcatcttaa aaatacccct gacgtttttt cagctccata gaccccgcct
    60721 ggtcgcggtc aattacgcgg ccctgacgtt cactggtggt ggaaaccagc atctcttcga
    60781 tgatctccgg cagtgtttgc gcatttgact ccaccgcgcc ggtcagcggc caacagccca
    60841 gtgtgcggaa acgcaccatc cgttttttaa tcacttcgcc cggttgcagg tcgatacggt
    60901 tgtcatcaat catcatcaac ataccgtcgc gttccagaac cggacgttcc gcagcaagat
    60961 acagcggaac aatgtcgata ttttccagcc agatgtattg ccagatatcc tgctcggtcc
    61021 agttagagag cgggaagacg cggatgcttt cgcctttgtt aatttgcccg ttgtagttgt
    61081 gccacagctc cgggcgctga tttttcggat cccagcgatg gaagcggtca cggaaagagt
    61141 agattcgctc tttagcgcgg gatttctctt cgtcacggcg cgcaccaccg aaggcggcat
    61201 caaaaccgta tttgttcagc gcctgtttca ggccttcagt tttcataata tcggtatgtt
    61261 tcgcgctgcc gtgtacgaat ggattaatcc ccatcgccac gccttccggg tttttatgca
    61321 ccagcagctc gcagccgtag gctttcgccg tacgatcgcg gaactcatac atctcgcgga
    61381 atttccagcc ggtatcgaca tgcagcaacg ggaaaggcag cgtacctgga taaaacgcct
    61441 tgcgcgccag atgcagcatg acgctggaat ctttaccgat agagtagagc atcaccggat
    61501 ttgagaattc tgcagccacc tcgcgaataa tgtggatgct ttccgcctcc agttgccgca
    61561 ggtgggtaag tcgtttttga tccataaccg ttcctttgca ataccgctat tttcttgcca
    61621 tcagatgttt cgactatagg gagcgtaaga gaacgaatga aattaccaat tagaatgagt
    61681 agttccttaa cggaataacg atttggcaaa gctaatatca aaaagtgctt aaggcaccgg
    61741 atttcgggcg tttaggaaga tttgaaattg ttttagcgca gcggcagttt catactatga
    61801 cggtaaaaaa atttgcatgg tatttaagga ctcactatgt tttccgcatt gcgccaccgt
    61861 accgctgccc tggcgctcgg cgtatgcttt atcctccccg tacacgcctc gtcacctaaa
    61921 cctggcgatt ttgctaatac ccaggcacga catattgcta ctttctttcc gggacgcatg
    61981 accggaactc ctgcagaaat gttatctgcc gattatattc gccaacagtt tcagcaaatg
    62041 ggttatcgca gtgatattcg gacatttaat agtcggtata tttataccgc ccgcgataat
    62101 cgcaagagct ggcataacgt gacgggaagt acggtgattg ccgctcatga aggcaaagct
    62161 ccgcagcaga tcatcattat ggcgcatctg gatacttacg ctccgctgag cgatgctgac
    62221 gccgatgcca atctcggcgg gctgacgtta caaggaatag atgataacgc cgcaggttta
    62281 ggtgtcatgc tggaactggc agaacgcctg aaaaatacgc ctaccgagta tggtattcga
    62341 tttgtggcga ccagtggtga agaggaaggg aaattaggtg ctgagaattt actcaagcgg
    62401 atgagtgaca ccgaaaagaa aaatacgctg ctggtgatta atctcgataa cttaattgtt
    62461 ggcgataaat tgtatttcaa tagtggtgta aaaacccctg aagcagtaag gaaattaacg
    62521 cgcgacaggg cgctggcaat tgcgcgtagt catggaattg ccgcaacgac caatccgggt
    62581 ttgaataaaa attatccgaa aggcactggg tgttgtaatg acgcagaaat attcgacaaa
    62641 gccggtattg ctgtactttc ggtggaagcg actaactgga atcttgggaa taaggatggt
    62701 tatcagcaac gcgcaaaaac agccgcattc cctgcgggaa atagctggca tgacgtaaga
    62761 ctggataatc aacaacatat tgataaggcg cttcctggaa gaatagaacg tcgctgccgt
    62821 gatgttatgc ggataatgct acctctggtg aaggagttgg cgaaggcgtc ttgatgggtt
    62881 tgaaaatggg agttgggagt tctaccgcag gggcggggaa ttctaagtga tatccatcat
    62941 cgcatccagt gcgcccggtt tatccccgct gacgcgggga acacacatga atgtcggttc
    63001 agaccgtgtt tttacccggt ttatccccgc tggcgcgggg aacacgaggc tgaacaggga
    63061 gggggctacc ggcacagcgg tttatccccg ctggcgcggg gaacacttgc caaccgccaa
    63121 agactggaaa accaatctcg gtttatcccc gctggcgcgg ggaacacgaa ctggcgttga
    63181 gtgataaatt tgacggaatc ggtttatccc cgctggcgcg gggaactctt cagtttcaca
    63241 ctcggtaatg ccgttattta cggtttatcc ccgctggcgc ggggaactcc tcggggccag
    63301 cctcccccat taacccggca ccggtttatc cccgctggcg cggggaactc tctaaaagta
    63361 tacatttgtt cttaaagcat tttttcccat aaaaacaacc caccaacctt aatgtaacat
    63421 ttccttatta ttaaagatca gctaattctt tgtttttaaa caggtaaaaa agagaccaac
    63481 cttaaaccat ccaaatctac cggagtacgc ctgtttaccc caaatgtctg gaactcaaat
    63541 cccgattccg tattcgtagc ccatgccatc accacattgc cttcttccgc cagtccagat
    63601 atttgttccc agatcatttc acggactctt gcggatacat cacctacata cacccctgca
    63661 cgtacctcca acaaccagat ggctaatctg cctcgtaagc gcgaaggtac attttcagtg
    63721 accacgacca acatactcat ctcagctact ccgatgtccg gcatctccca gagaaacggg
    63781 aagtggaata gcaacaggct gcgcatcttc aggtggggcc ggcggctgta tttccccagc
    63841 agcaagcacg tcctctataa gcggaatcaa tttgactaat gttttactac tgcgaaaaat
    63901 atccctgcac gccaaacgga cttcccggtc cggctcacca ggaatacgac gcgctatctc
    63961 aaaagctttc ggtacaacag tgtcaaattt aatgatgtcc gcgatatcgt aaacaaagga
    64021 aagaggcttt cctgtatgca caaacccaat agctggtgca taaccagctg caagtatcgc
    64081 cgcttcagtt acgccatata aacaagaagt tgcagcgcta atgcattggt tgaccgtatc
    64141 gcccttttcc cagtctttcg gatcgtaacg acgtccattc catttcaccc cgtattgctt
    64201 cgccagaagt acataggttg ctcgcacacg acttccttct atacctctga gttgctctac
    64261 ggagcgccgg gcaggcgcag gttctccaaa ccgaagttca aacattttac gtacgacctt
    64321 cagacgcaaa tcttcatcca gagcaagttt tgcctgatag agcagcttat ctgaacgcgc
    64381 accaccaggc tgaccagaag cataaacacg aacgcccgct tcccccaccc ataccaataa
    64441 tgttccaact tgcgcagcca ggcgtacagc tgcatgcgaa acccgtgtac caggttccaa
    64501 catgatgcag gcaaccgagc caacaggaat atgagtgcgg atccctgtct tgtcgataag
    64561 tacaaacgcg ccatctatta catcgatctg cccatattgc agaaagatca tggagacgcg
    64621 atctttgagt ggaatgggat taaggggaag ccaggtcatt ttattacacc tcaatcacag
    64681 tggagccaaa gatagcaagc cacatcccat cgatttagct ggcccaatac cttgctgtac
    64741 aagatctatt aacgctggcg cgtcgttgat ggtgatcacc ccttcaaagc aaaccgtttg
    64801 gatctttcca cttttaccat caccagaaaa atactgtggc cgttccgata tgggatgcac
    64861 atcttcaacg cgcgccgcat tgcccaattt acgttgcaac cacgcgattt gttctgcttc
    64921 ttttattaac ggaacccgac agcgtttaat attcccttta ctgtccaggc gcttttgatt
    64981 gtcgagaata gttttgatcg gatttgcccg aagccgaaaa tagagtggaa caccaacctg
    65041 aagttgaaat tcaacctgtt tagttttaat gactgtcgca acggcagtag aaacaggcat
    65101 ttgcgctgac tgcaataaaa catgacagcc ttctggtgtg tttcgcttct caacatgaaa
    65161 aagaaaatca cgagcagcat ccggtctgtt tggaaataaa tgccataatc cctggtgaag
    65221 ttggtaaaga tccctgctcc aggccctggc aatgatgact ttactgagat acatccatac
    65281 ctcctttaat cacataccat tctcgggaag caaattgtcg aggtaaggtg atcatcggtt
    65341 cgtcgcgcac cgtaaatttt aaatgatgcc cgtcaactga ttcctcacta taaatatcgc
    65401 cgccaacggg ctcataattt aatagcgcct tctgaggatc cgacgcctga catgtcccga
    65461 gaaaaagcgg ttgtgttagc ggacaactcc tccgccccag atacggtgta taccgaggct
    65521 ttaatactgc tttttcaagt tctgacataa ccatcgttgc ctgtggtgtt aaccagatag
    65581 cgacggtaaa ggaggcatca cataaatatt cgcgccatgt ttgaatcgtt tcatgacttt
    65641 tcaaaccacg gtaatcttct cgcgctccaa ggactgtatg gtaatcacgc aaccccgtta
    65701 cagacacacg acgatcgtca agaatgagtt catcgcagcg cactgcaaat tgcacactct
    65761 ctgataacgc ctgtaatgaa gaagtatcat cacgttggat cccaagacaa gccccgagta
    65821 gccctaataa cccgcttcgg gtcggaaatc ttccggtagg tcgcgttcct tcaaaggtcg
    65881 gctgccccca ggcttgcatt ggcccagcaa gccgcaagat caaataagat ctcatgttca
    65941 cgcctcgcca ttattacgaa cccaggattt taactgttct aaagtaggca tttgtttaac
    66001 ttgagcagta attgggtcta catcagataa gctgaattgc gcagcagctc cgttcagacc
    66061 atatccattg gcaacgcgat cccaatattg attaaacgcc tgtatagacg gttgcaaaaa
    66121 gccatctttc gctttaaccg ctttttcaaa agcatttgcc atagaaagtg gcatatcgga
    66181 gaaattaacc attaccatat ccgcagggtt aaaagcggca taagtacgct gttttgctcc
    66241 agggacctct gttgccagca tatgaacaac atgggttgca atttccagag cctgctccct
    66301 ggaggcacca cctaaatttt cctgaagttg agcgaggtta atgttggcat aacgataaaa
    66361 aacacccgat gaaaattcct gagttcccag atgtgcagaa ccttgttcct gtaaatcatc
    66421 tacagcggtg aaccagtcaa tatcagaatc aacctgatga gtagtgatcg catgcgcaat
    66481 ggacattgca ccatcaactt ttcccaactc actcatcatg ccgctggttg ccattcttcc
    66541 actaagcgca atatcaacac cctgctgtaa attcacacgt atggcggcaa tatcttcctt
    66601 aagaactttg agcagctttt tatcatccag attatcagcc tctgcttttg caacctgctc
    66661 acagaaccag gctatttctc ccacaaccca gggagtaacc gcatcggcag aaatcttttc
    66721 ggcttcatca actaatttac cggagagcag cgctaatgtc ttatcgatga ttttttggtc
    66781 aaaacgttca ccaagttttt gccgaagaac atcacgtaat tgtgcaagat gaattgttct
    66841 gagactggat tcaccaatat tttgtgcgta ataatcactt ttacgcatcg catgtttaag
    66901 gctttgactt gaaattctta ctcgtctttt cccgccgaaa atagcgtctt tctgcatgtt
    66961 catatcgtcg cggttcagac atgaagggct gtgagagatc agaacatgaa tattgataaa
    67021 gttagacata gaaaggtttc cttacgcatt tttgtttgtg gtcaatacaa aatcttccag
    67081 aagttgctgg cgttcgcgct ttccccacca ggtcaacatc ctggccatta atggccagtc
    67141 aagtacgggt tcggcgtgag taagtaatcg acgtaactgg accatatcgg ctgttctgtc
    67201 agcccgaatt aattgaaaga tacggcgctc gttaattctt ccactattgg ctaaagctct
    67261 tcccaacgag atacctgttg tttgctccgt ttttttgtcc tgatgtcgga tgacattctt
    67321 tcctgcgctc aggcaaaaca ccatgcgcaa aagagcctgc tggtgacgtg ggttttccca
    67381 accaaaagat tgcaccagcc tataaaacgc agggatatcg cgtaattcat caggttctga
    67441 aacacgtcta atttgcgcac atgctccatt atccagttgt tgccaggctc gatataaagc
    67501 cattgcatca atttcatcag ccatttgatg gccctccttg aggttttaac tcccgtaaat
    67561 gtttgtatag cgtggcgcgg gcaagcgcta atgtgcttat taatttagga tgatgtgcat
    67621 agggagctac agattgatta aatagcattt cacacaattg atgaagtttg tctcgtaaat
    67681 cagctattac ctcatcagct tgggaaaaat taatattcgc caatacatcg ggaattaata
    67741 attcactctg tcgatagaaa tgcctttctg cagtctcatg aacagaaact ccggcccctt
    67801 tgaagtcttt atttttaaac ccttctgcaa aggtatataa cgccttgcgt aaggctgttt
    67861 tatatcccaa accaacagtc actatttcgt ttatcacatt gccgtattgt tgccacccct
    67921 gattaaacat caacacatca tgacgccgtt caagaataga tgcttgatta ttacgatatc
    67981 cccccataat caattcaaga ggactttgcg gcgcaatatt tctgaattga ttcacaaccg
    68041 ccgccacgcg atttccattt tcattttgaa taatcttatc taccacaact cggctgattt
    68101 gtgtccatga tggtgcggag gtggtgaaag caagaaattt ttcctcaacc tcccctttct
    68161 tgactgttac cagacaaggg gaatgcggat ggggccatag cccattaact gtaaaggtaa
    68221 atttttcctt aagaaaaccg gtataacgca aattgctttc ctgaccacag caagaacatt
    68281 taccaatccc aatgggatcg catagttcaa tatgcgctgg ttgccagaat aaaccacgga
    68341 caaacccaat tgaagaagca ggcaccgatt cattcggctt gacaggctta acccaggtag
    68401 gttggttttc cgtatgtaat tcattgggaa attgtttttg aagacgaggt attgtgagga
    68461 cattgagtaa taccgtcgag cgaagatcga tcccacatac gaaggtggtt ataggtgttc
    68521 ctccacgtaa accgctttta aagccaccac caaaaccagg tgcctgattc gcctggttga
    68581 ataacgcaat cgcagtgcat ccaccacata atgcttcacc ctgccccggt tgattgacaa
    68641 atgcacaatt cgtcgcgccg cttaccccag ccaacagttt ttccattgga gtcacatcat
    68701 ttgctttgac acctttggtc tgcataaagg gatgttctgc gtgattaagg tagaacatat
    68761 ctatccacgg cgcgatgagt tgttgaaact catcttcagt gagcggattc attatgcgat
    68821 gtcgaaattc aacgtcatct tttgccgggg cgataatttg cccaatgcaa accagcagtg
    68881 ctaaagtggc cagttccata tcgtcacggg gcaaacttaa tcgccactga tctctactgc
    68941 agtatagcga ttgcagattt atgatttgga ctttcccccc gtttcgcggg cgtacaggga
    69001 tccagttatc aataagcaaa ttcatttgtt ctccttcata tgctccgaca tttctcctgc
    69061 atttctatgc gtcggcacac ttccgttatc ggtaaaaaca aagaaaaaat acggcgtaat
    69121 taattcaata atcacattca ctgcaaaaat atattcattg gtttaataca attaacctat
    69181 acatatatta ag
//
LOCUS       DAZGQL010000013        63605 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.013, whole genome shotgun sequence.
ACCESSION   DAZGQL010000013 DAZGQL010000000
VERSION     DAZGQL010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 63605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63605
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.013"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(25..231)
                     /gene="ybfA"
                     /locus_tag="ACHKIR_001011"
     CDS             complement(25..231)
                     /gene="ybfA"
                     /locus_tag="ACHKIR_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HFO9988787.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            544..633
                     /gene="kdpF"
                     /locus_tag="ACHKIR_001012"
     CDS             544..633
                     /gene="kdpF"
                     /locus_tag="ACHKIR_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HFO9988788.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            633..2306
                     /gene="kdpA"
                     /locus_tag="ACHKIR_001013"
     CDS             633..2306
                     /gene="kdpA"
                     /locus_tag="ACHKIR_001013"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HFO9988789.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTTGVER
                     VLFRALGVSDREMNWKQYLSAILGLNILGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFALIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVAGD
                     RRQGRMLLWAMSVIFIICTGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLALMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2329..4377
                     /gene="kdpB"
                     /locus_tag="ACHKIR_001014"
     CDS             2329..4377
                     /gene="kdpB"
                     /locus_tag="ACHKIR_001014"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HFO9988790.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTFISIAMASGAMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVEEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHVEANGGHFPVDVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            4386..4958
                     /gene="kdpC"
                     /locus_tag="ACHKIR_001015"
     CDS             4386..4958
                     /gene="kdpC"
                     /locus_tag="ACHKIR_001015"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HFO9988791.1"
                     /translation="MRGLRPALSTFLFLLLITGGVYPLLTTALGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASANVPVELVTASASGLDNNITPQAAAWQIPRVAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            4951..7635
                     /gene="kdpD"
                     /locus_tag="ACHKIR_001016"
     CDS             4951..7635
                     /gene="kdpD"
                     /locus_tag="ACHKIR_001016"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HFO9988792.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPLKRQAYRGRHISEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSSQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANALERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHVEGEKLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEEFHEDM"
     gene            7632..8309
                     /gene="kdpE"
                     /locus_tag="ACHKIR_001017"
     CDS             7632..8309
                     /gene="kdpE"
                     /locus_tag="ACHKIR_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HFO9988793.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVFEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSAVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFML"
     gene            8543..8647
                     /locus_tag="ACHKIR_001018"
     CDS             8543..8647
                     /locus_tag="ACHKIR_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9988794.1"
                     /translation="MENNSRTMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            8905..11103
                     /gene="speF"
                     /locus_tag="ACHKIR_001019"
     CDS             8905..11103
                     /gene="speF"
                     /locus_tag="ACHKIR_001019"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004586 - ornithine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HFO9988795.1"
                     /translation="MSKLKIAVSDSCPDCFTTQRECIYINENSNIDVAAIVLSLNDVT
                     RGKLDEIDATGYGIPVFIATENQERVPAEYLPRISGVFENCESRREFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEALFRADLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELIAEFAPQR
                     AKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELV
                     DGKPWQSYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     ESDAPLSEVLPSIYKQHEERYTGYTLRQLCQEMHDLYARHNVKQLQKEMFRKEHFPRV
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            11100..12419
                     /gene="potE"
                     /locus_tag="ACHKIR_001020"
     CDS             11100..12419
                     /gene="potE"
                     /locus_tag="ACHKIR_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015496 - putrescine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:0015847 - putrescine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HFO9988796.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYANSWNPHHVPFFSAVGSSIAMTLWAFLGLESACANAEVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSADEGYFPKIFSRVSKVDAPVQGMLTIVIIQS
                     GLSLMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(12476..14116)
                     /gene="pgm"
                     /locus_tag="ACHKIR_001021"
     CDS             complement(12476..14116)
                     /gene="pgm"
                     /locus_tag="ACHKIR_001021"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HFO9988797.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAISNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALLADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(14142..14687)
                     /gene="seqA"
                     /locus_tag="ACHKIR_001022"
     CDS             complement(14142..14687)
                     /gene="seqA"
                     /locus_tag="ACHKIR_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HFO9988798.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            14872..15636
                     /gene="ybfF"
                     /locus_tag="ACHKIR_001023"
     CDS             14872..15636
                     /gene="ybfF"
                     /locus_tag="ACHKIR_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO9988799.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPTLFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            15776..16069
                     /gene="ybfE"
                     /locus_tag="ACHKIR_001024"
     CDS             15776..16069
                     /gene="ybfE"
                     /locus_tag="ACHKIR_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HFO9988800.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            16209..16739
                     /gene="fldA"
                     /locus_tag="ACHKIR_001025"
     CDS             16209..16739
                     /gene="fldA"
                     /locus_tag="ACHKIR_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HFO9988801.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            16949..17035
                     /gene="uof"
                     /locus_tag="ACHKIR_001026"
     CDS             16949..17035
                     /gene="uof"
                     /locus_tag="ACHKIR_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HFO9988802.1"
                     /translation="MIRIISRANSVTSPNEVNRLVTGQIPHD"
     gene            17028..17474
                     /gene="fur"
                     /locus_tag="ACHKIR_001027"
     CDS             17028..17474
                     /gene="fur"
                     /locus_tag="ACHKIR_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HFO9988803.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(17558..17884)
                     /gene="chiQ"
                     /locus_tag="ACHKIR_001028"
     CDS             complement(17558..17884)
                     /gene="chiQ"
                     /locus_tag="ACHKIR_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChiQ/YbfN family lipoprotein"
                     /protein_id="HFO9988804.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFAEQESVRVLDYQQCIQATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(17934..19340)
                     /gene="chiP"
                     /locus_tag="ACHKIR_001029"
     CDS             complement(17934..19340)
                     /gene="chiP"
                     /locus_tag="ACHKIR_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HFO9988805.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSIGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDIYDGTAWLQALTFGYRAADVVDLRLEGSWVKADGQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFGAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
     gene            complement(19789..21453)
                     /gene="glnS"
                     /locus_tag="ACHKIR_001030"
     CDS             complement(19789..21453)
                     /gene="glnS"
                     /locus_tag="ACHKIR_001030"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HFO9988806.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRTGSFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSCEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(21656..23602)
                     /gene="nagE"
                     /locus_tag="ACHKIR_001031"
     CDS             complement(21656..23602)
                     /gene="nagE"
                     /locus_tag="ACHKIR_001031"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HFO9988807.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLNQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVVDSARVNDVMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAETAPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            23935..24735
                     /gene="nagB"
                     /locus_tag="ACHKIR_001032"
     CDS             23935..24735
                     /gene="nagB"
                     /locus_tag="ACHKIR_001032"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFO9988808.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            24795..25943
                     /gene="nagA"
                     /locus_tag="ACHKIR_001033"
     CDS             24795..25943
                     /gene="nagA"
                     /locus_tag="ACHKIR_001033"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFO9988809.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTAFTPDFKITRTIVNGNEVVTQ"
     gene            25952..27172
                     /gene="nagC"
                     /locus_tag="ACHKIR_001034"
                     /gene_synonym="nagR"
     CDS             25952..27172
                     /gene="nagC"
                     /locus_tag="ACHKIR_001034"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HFO9988810.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            27220..27972
                     /gene="nagD"
                     /locus_tag="ACHKIR_001035"
     CDS             27220..27972
                     /gene="nagD"
                     /locus_tag="ACHKIR_001035"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HFO9988811.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            28228..29892
                     /gene="asnB"
                     /locus_tag="ACHKIR_001036"
     CDS             28228..29892
                     /gene="asnB"
                     /locus_tag="ACHKIR_001036"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HFO9988812.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKALVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDYDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFEAYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            30273..30349
                     /locus_tag="ACHKIR_001037"
     tRNA            30273..30349
                     /locus_tag="ACHKIR_001037"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30307..30309,aa:Met,seq:cat)
     gene            30358..30442
                     /locus_tag="ACHKIR_001038"
     tRNA            30358..30442
                     /locus_tag="ACHKIR_001038"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30392..30394,aa:Leu,seq:tag)
     gene            30466..30540
                     /locus_tag="ACHKIR_001039"
     tRNA            30466..30540
                     /locus_tag="ACHKIR_001039"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30498..30500,aa:Gln,seq:ttg)
     gene            30575..30649
                     /locus_tag="ACHKIR_001040"
     tRNA            30575..30649
                     /locus_tag="ACHKIR_001040"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30607..30609,aa:Gln,seq:ttg)
     gene            30665..30741
                     /locus_tag="ACHKIR_001041"
     tRNA            30665..30741
                     /locus_tag="ACHKIR_001041"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30699..30701,aa:Met,seq:cat)
     gene            30790..30864
                     /locus_tag="ACHKIR_001042"
     tRNA            30790..30864
                     /locus_tag="ACHKIR_001042"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30822..30824,aa:Gln,seq:ctg)
     gene            30902..30976
                     /locus_tag="ACHKIR_001043"
     tRNA            30902..30976
                     /locus_tag="ACHKIR_001043"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30934..30936,aa:Gln,seq:ctg)
     gene            complement(31130..32305)
                     /gene="ubiF"
                     /locus_tag="ACHKIR_001044"
     CDS             complement(31130..32305)
                     /gene="ubiF"
                     /locus_tag="ACHKIR_001044"
                     /EC_number="1.14.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-demethoxyubiquinol 3-hydroxylase"
                     /protein_id="HFO9988813.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVMVIEHAQPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLIALHRHNDLQELELKGGETILAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLIRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFMMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRKALKYALGL"
     gene            32451..33875
                     /gene="miaB"
                     /locus_tag="ACHKIR_001045"
     CDS             32451..33875
                     /gene="miaB"
                     /locus_tag="ACHKIR_001045"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HFO9988814.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            34028..35068
                     /gene="ybeZ"
                     /locus_tag="ACHKIR_001046"
     CDS             34028..35068
                     /gene="ybeZ"
                     /locus_tag="ACHKIR_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFO9988815.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            35065..35532
                     /gene="ybeY"
                     /locus_tag="ACHKIR_001047"
     CDS             35065..35532
                     /gene="ybeY"
                     /locus_tag="ACHKIR_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFO9988816.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEAIETEIMLALGYEDPYIAEKE"
     gene            35622..36500
                     /gene="corC"
                     /locus_tag="ACHKIR_001048"
     CDS             35622..36500
                     /gene="corC"
                     /locus_tag="ACHKIR_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HFO9988817.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            36525..38063
                     /gene="lnt"
                     /locus_tag="ACHKIR_001049"
     CDS             36525..38063
                     /gene="lnt"
                     /locus_tag="ACHKIR_001049"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HFO9988818.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDGDQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSLNGIQLTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     ARKH"
     gene            complement(38141..40018)
                     /locus_tag="ACHKIR_001050"
     CDS             complement(38141..40018)
                     /locus_tag="ACHKIR_001050"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFO9988819.1"
                     /translation="MSASSVKPLNVQLPAITLILFALCVGIFCYLAQWMSYEEVDQSA
                     LIHLGANVASLTLSGESWRLLSSVFLHSSVSHLLMNMFALLVVGGAVERILGKWRLLI
                     IWLFSGIFGGLISACYALRESEQIVISVGASGAIMGIAGAAIATQLSSGAGTHHKNQR
                     RVFSLLGMVALTLLYGARQAGIDNACHIGGLIAGGALGWLSARLVGQNRLVTEGGIIV
                     AVTLLLTGTIWFVQQQIDESVLQVRQSLREAFYPQEIEQERRQKKQQLVEERNALRET
                     LSAPVSREQASGDLLAEIADIHDMAISRDGNTLYAAIENTNSIVVFDLGQKKILHTFT
                     APIAKEKSVKHCGGCKDQGVRSLALSLDEKLIYATSFEANAVSVINVATGEIIQSITT
                     GAHPDSLILSRDGTKAWVMNRTSNSVSAIDLVTYQHVTDIPLEKYDGTGTSGKPGAWV
                     MALSPDEKTLLIPGMVRGDIVRINTITHQKEDFPTGDARGIVSAMGFRPKNGEIIFAD
                     SQGISRISVGDQQASIMTQWCSRSVYSVEGISPDGQYLALVSYGLQGYVILLNINAGQ
                     IIGVYPASYVNHLRFSADGRKIFVMAKNRLIQMDRTRSLDPQAIIRHPQYGDVACIPE
                     P"
     gene            40473..41381
                     /gene="gltI"
                     /locus_tag="ACHKIR_001051"
     CDS             40473..41381
                     /gene="gltI"
                     /locus_tag="ACHKIR_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HFO9988820.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNAERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWEIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            41551..42291
                     /gene="gltJ"
                     /locus_tag="ACHKIR_001052"
     CDS             41551..42291
                     /gene="gltJ"
                     /locus_tag="ACHKIR_001052"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HFO9988821.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAFIMLVMTLVERKVRLPGNMGGK"
     gene            42291..42965
                     /gene="gltK"
                     /locus_tag="ACHKIR_001053"
     CDS             42291..42965
                     /gene="gltK"
                     /locus_tag="ACHKIR_001053"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HFO9988822.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            42965..43690
                     /gene="gltL"
                     /locus_tag="ACHKIR_001054"
     CDS             42965..43690
                     /gene="gltL"
                     /locus_tag="ACHKIR_001054"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP-binding
                     protein GltL"
                     /protein_id="HFO9988823.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            43808..44743
                     /gene="rihA"
                     /locus_tag="ACHKIR_001055"
     CDS             43808..44743
                     /gene="rihA"
                     /locus_tag="ACHKIR_001055"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050263 - ribosylpyrimidine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HFO9988824.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRNLIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLCESEEPITIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            complement(44788..45270)
                     /gene="ybeL"
                     /locus_tag="ACHKIR_001056"
     CDS             complement(44788..45270)
                     /gene="ybeL"
                     /locus_tag="ACHKIR_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HFO9988825.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            45505..48087
                     /gene="leuS"
                     /locus_tag="ACHKIR_001057"
     CDS             45505..48087
                     /gene="leuS"
                     /locus_tag="ACHKIR_001057"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFO9988826.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSKAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            48102..48683
                     /gene="lptE"
                     /locus_tag="ACHKIR_001058"
     CDS             48102..48683
                     /gene="lptE"
                     /locus_tag="ACHKIR_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HFO9988827.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            48683..49714
                     /gene="holA"
                     /locus_tag="ACHKIR_001059"
     CDS             48683..49714
                     /gene="holA"
                     /locus_tag="ACHKIR_001059"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFO9988828.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAIRQVAAV
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAVRAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHMPLRALFDKH
                     RVWQNRRGMMGEALNRLSHPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            49716..50357
                     /gene="nadD"
                     /locus_tag="ACHKIR_001060"
     CDS             49716..50357
                     /gene="nadD"
                     /locus_tag="ACHKIR_001060"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFO9988829.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSMQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            50381..50992
                     /gene="cobC"
                     /locus_tag="ACHKIR_001061"
     CDS             50381..50992
                     /gene="cobC"
                     /locus_tag="ACHKIR_001061"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HFO9988830.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLHD
                     VSFDLVLCSELERAQHTARLVLSDRQHPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILVVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            51252..51569
                     /gene="rsfS"
                     /locus_tag="ACHKIR_001062"
     CDS             51252..51569
                     /gene="rsfS"
                     /locus_tag="ACHKIR_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFO9988831.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            51573..52040
                     /gene="rlmH"
                     /locus_tag="ACHKIR_001063"
     CDS             51573..52040
                     /gene="rlmH"
                     /locus_tag="ACHKIR_001063"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFO9988832.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            52071..53972
                     /gene="mrdA"
                     /locus_tag="ACHKIR_001064"
     CDS             52071..53972
                     /gene="mrdA"
                     /locus_tag="ACHKIR_001064"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HFO9988833.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            53975..55087
                     /gene="mrdB"
                     /locus_tag="ACHKIR_001065"
                     /gene_synonym="rodA"
     CDS             53975..55087
                     /gene="mrdB"
                     /locus_tag="ACHKIR_001065"
                     /gene_synonym="rodA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HFO9988834.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTAIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            55098..56186
                     /gene="rlpA"
                     /locus_tag="ACHKIR_001066"
     CDS             55098..56186
                     /gene="rlpA"
                     /locus_tag="ACHKIR_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HFO9988835.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGLYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVPVPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            56326..57537
                     /gene="dacA"
                     /locus_tag="ACHKIR_001067"
     CDS             56326..57537
                     /gene="dacA"
                     /locus_tag="ACHKIR_001067"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HFO9988836.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            57647..57910
                     /gene="ybeD"
                     /locus_tag="ACHKIR_001068"
     CDS             57647..57910
                     /gene="ybeD"
                     /locus_tag="ACHKIR_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein YbeD"
                     /protein_id="HFO9988837.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            58011..58652
                     /gene="lipB"
                     /locus_tag="ACHKIR_001069"
     CDS             58011..58652
                     /gene="lipB"
                     /locus_tag="ACHKIR_001069"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HFO9988838.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            58912..59435
                     /locus_tag="ACHKIR_001070"
                     /pseudo
     CDS             58912..59435
                     /locus_tag="ACHKIR_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YbeF family transcriptional regulator"
     gene            59676..60641
                     /gene="lipA"
                     /locus_tag="ACHKIR_001071"
     CDS             59676..60641
                     /gene="lipA"
                     /locus_tag="ACHKIR_001071"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HFO9988839.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(60742..60945)
                     /gene="tatE"
                     /locus_tag="ACHKIR_001072"
     CDS             complement(60742..60945)
                     /gene="tatE"
                     /locus_tag="ACHKIR_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HFO9988840.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(61074..61862)
                     /locus_tag="ACHKIR_001073"
     CDS             complement(61074..61862)
                     /locus_tag="ACHKIR_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HFO9988841.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENEVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLDRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRHVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARVLDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFALPQLL"
     gene            61955..62338
                     /gene="crcB"
                     /locus_tag="ACHKIR_001074"
     CDS             61955..62338
                     /gene="crcB"
                     /locus_tag="ACHKIR_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HFO9988842.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(62392..62601)
                     /gene="cspE"
                     /locus_tag="ACHKIR_001075"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(62392..62601)
                     /gene="cspE"
                     /locus_tag="ACHKIR_001075"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFO9988843.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(62776..63336)
                     /gene="pagP"
                     /locus_tag="ACHKIR_001076"
     CDS             complement(62776..63336)
                     /gene="pagP"
                     /locus_tag="ACHKIR_001076"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HFO9988844.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADEWMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGQSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYDP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
BASE COUNT        15399 a        16356 c        16315 g        15535 t
ORIGIN      
        1 ttcaatgtat tcagaactca gcaatcagta aaaatcacca gtcgcctttt cggcctgatc
       61 catccacacc ggtttatcgc tggttttcga ccagacgcgg tgcaggtagc tgtaaaagcg
      121 ggcgcggtct ttccagaaaa gcatgaaagg cagcgccaga acgcccgctg caaccgcata
      181 agtacggcgt aaaaagataa gccatgcagg atattctctg tagagttcca tacttttctc
      241 ccccttttgt atctacccgg tgaatagcac cggaaaaatg aatttgttta tctgatgaaa
      301 atagtaccgc cttttgtgta attttactac tcatccgacc acttattttt gcttattgat
      361 ggtttattta cattcatacc gtaattaagt tacacaaagg ttaaattaat actaaacatt
      421 agttaaatca tggcttttgc catttttata ctttttttac accccgcccg cagatttttg
      481 cgaaatcttt gcagccagaa ttctactctt ccggtatcac ttttaggcca ctggaggtgc
      541 actgtgagtg caggcgtgat agccggcgtg ttgctggtgt ttttattact gggttatctg
      601 gtttatgccc tgatcaatgc ggaggcgttc tgatggctgc gcaagggttc ttactgattg
      661 ccacgttttt actggtgtta atggtgctgg cgcgtccttt aggcagcggg ctggcgcggc
      721 tgattaatga cattcctctt cccggtacga cgggcgttga gcgcgtactt tttcgcgcgc
      781 ttggcgtctc tgaccgcgag atgaactgga agcagtatct ctcagccatt cttggcctga
      841 atatactggg gctggcggtg ctgtttttta tgttgctcgg tcagcactat ctgccgctta
      901 atccacagca gttgccagga ctgtcgtggg atctggcgct gaataccgcc gtcagctttg
      961 tcacaaatac caactggcaa tcttacagcg gtgaaaccac gttgagctat ttcagccaga
     1021 tggcgggctt aacggtgcaa aactttcttt ctgccgccag cgggattgcg gtgatttttg
     1081 ccctcatacg tgcatttacc cgccagagca tgagcacact cgggaatgcc tgggtcgatc
     1141 tgctacgcat cacgttatgg gtgctagtcc ctgtggcgtt gttgatcgca ttgtttttta
     1201 ttcaacaagg tgcgctgcaa aactttctgc cctatcaggc ggtgaatacc gttgaaggag
     1261 cgcaacagct gttacccatg gggcctgtag cttctcagga agcgatcaag atgctcggca
     1321 ctaacggcgg tggcttcttt aatgccaact cgtcgcatcc gtttgaaaac ccaaccgcac
     1381 tgaccaactt cgtgcagatg ctggcgatct tcttgatccc aacagcgctg tgctttgcct
     1441 ttggcgaagt ggcgggcgat cgccgtcagg ggcgcatgtt gctgtgggcg atgtcagtga
     1501 tttttatcat ctgcacaggc gtggtgatgt gggcagaagt tcagggtaat ccgcatctgc
     1561 tggcactggg cgcggacagc agcatcaata tggaaggtaa agagagtcgt ttcggcgtgc
     1621 tggtcagtag cctgtttgcg gtcgtgacta cggcggcttc ctgtggcgcg gtgattgcga
     1681 tgcatgattc gtttaccgct cttggtggca tggtgccgat gtggctgatg caaattggtg
     1741 aagtggtgtt cggcggtgtc ggttctggtc tttacggcat gatgctgttt gtcctgctgg
     1801 cggtgtttat tgctgggctg atgattggtc gtacaccgga atatctgggt aaaaaaatcg
     1861 acgtacgcga gatgaaactg accgcgctgg cgattctggt caccccaacg ctggcgctga
     1921 tgggcgcggc gctggcaatg atgaccgacg ccggacgtag cgccatgctc aaccctggcc
     1981 cgcatggttt tagcgaagtg ctgtacgccg tgtcatccgc cgctaacaac aacggcagcg
     2041 cctttgccgg attaagcgcc aactctccat tctggaactg tttgctggcg ttctgcatgt
     2101 ttgtcggtcg cttcggggta attatcccgg tgatggcaat tgccggttcg ctggtgagta
     2161 aaaagagcca gcccgccagc tccggcacgc tgccaacgca cggcccgctg tttgttggcc
     2221 tgttaatcgg caccgtgttg ctggttggcg cactgacctt tatccctgcc ctggcgcttg
     2281 gtccggtggc ggaatatctc tcctgatgat attgagtgag cactgaatat gagtcgtaaa
     2341 caactggcgc tattcgaacc aacacttgtc gttcaggcgc tgaaagaagc ggtgaaaaaa
     2401 ttaaacccgc aggcgcaatg gcgtaatcca gtgatgttta tcgtctggat cggcagtctg
     2461 ttaaccacct ttattagcat tgcaatggca agcggcgcga tgcctggcaa tgcgctgttt
     2521 agcgcggcaa ttagcggctg gctgtgggtc accgtactgt tcgctaattt cgccgaggcg
     2581 ctggcagaag gccgcagtaa agcgcaagcc aatagtctga aaggggtgaa aaaaactgcc
     2641 tttgcccgca agctgcgtga gccgaaatat ggcgctgcgg cggacaaagt tcccgcagat
     2701 caactgcgta aaggcgatat cgtactggta gaagctggcg atattatccc ctgcgatggt
     2761 gaagtcattg aaggcggcgc atcggtcgat gaaagcgcca tcaccgggga atcggctccg
     2821 gtgatccgtg aatccggcgg cgattttgcc tccgtcactg gcggcacgcg tattctttct
     2881 gactggctgg tgattgagtg cagcgttaac cccggcgaga catttctgga tcggatgatc
     2941 gcgatggtgg aaggcgcgca gcgacgcaaa acgccgaacg agattgccct gaccatcctg
     3001 ctgattgccc tgactatcgt ctttttactg gcaaccgcca cgctgtggcc gttttccgca
     3061 tggggtggta atgcagtcag cgtaacggta ctggtggcgc tgttggtctg tctgatccca
     3121 accactattg gcggcctgtt gtcagcgatc ggcgtcgccg ggatgagccg gatgttgggc
     3181 gcgaatgtga ttgccaccag cgggcgtgca gttgaagcgg cgggcgacgt ggacgtcctg
     3241 ctgctggata aaactggcac catcaccctc ggtaaccgtc aggcatcaga gtttatccct
     3301 gcgcagggcg tagaggaaaa aacgctggcc gacgcagcac aactggcttc gctggccgac
     3361 gaaacgccgg aaggccgcag tattgtgatc ctcgccaagc agcgctttaa cctgcgcgag
     3421 cgcgatgtgc agtcgcttca cgccactttt gtaccgttta ctgcgcaaag tcgaatgagc
     3481 gggatcaata tcgacaaccg catgatccgt aaaggttctg tcgatgccat tcgccgccat
     3541 gttgaggcta acggtggtca cttccctgtc gatgttgatc aaaaagtcga tcaggttgcg
     3601 cgtcaggggg ccacgccgct ggtggtggtg gaaggttctc gtgtgctggg cgttattgcg
     3661 ctgaaagata tcgtcaaagg cggtattaaa gagcgcttcg cccagctgcg caaaatgggc
     3721 attaaaacgg tgatgattac cggcgataac cgtctgactg ccgccgccat tgctgcggaa
     3781 gcgggtgtcg atgattttct cgccgaagcg acaccggagg ccaagctggc gttaattcgt
     3841 cagtatcagg cggaaggccg tttggtggcg atgaccggcg acggcaccaa tgatgctccg
     3901 gcgctggcgc aggcagatgt cgcggtagcg atgaactccg gtactcaggc ggcgaaagaa
     3961 gcgggcaata tggtggatct cgactctaac ccgaccaagt tgatcgaggt ggtgcatatt
     4021 ggcaaacaga tgctgatgac ccgtggctcg ctgaccacct tcagcattgc caacgatgtg
     4081 gcgaaatact tcgccattat tccggcggcg ttcgcggcaa cgtatccgca gttaaatgcg
     4141 ctgaacatca tgcgcctgca ttcgcccgac tccgccattc tcagtgcggt cattttcaac
     4201 gccttgatta tcgtcttttt gattcccctg gcgttaaaag gcgtgagtta taaaccgctt
     4261 accgcttctg ccatgttgcg ccgtaactta tggatttacg gtctgggtgg gctgctggtg
     4321 ccgtttattg gtatcaaagt cattgattta ctgctgaccg tttgcggtct ggtgtgaggt
     4381 ttaccatgcg tggattacgt ccggcattat caacatttct ctttttgtta ctgattactg
     4441 gcggtgttta cccgctgctg accaccgcgc tggggcaatg gtggtttccc tggcaggcca
     4501 acggttcgtt gattcgcgaa ggtgatacgg tgcgcggctc ggcattgatc gggcagaatt
     4561 ttactggcaa cggctatttt catggtcgcc cgtcggcaac ggcagaaatg ccctataatc
     4621 cacaggcttc tggcgggagc aatctggcgg ttagtaaccc ggagctggat aaacaaatcg
     4681 ctgcacgcgt tgccgcatta cgggccgcta acccggatgc cagcgcgaac gttccggttg
     4741 aactggtgac ggcgtcggca agcgggctgg acaataacat caccccgcaa gcggcggcct
     4801 ggcagatccc acgcgtggcg aaagcgcgta atctcagcgt tgaacagctc acgcagctga
     4861 tcgcaaaata tagccaacaa ccgctggtga aatatatcgg ccagccggtc gtcaacattg
     4921 tcgaactcaa tctggcgctg gataagcttg atgaataacg aacccttacg tcccgacccc
     4981 gatcgtctgc tggaacaaac tgccgcgccg catcggggga agctgaaagt tttcttcggt
     5041 gcctgcgcag gcgtcggcaa gacctgggca atgctggcag aagcccagcg actgcgggcg
     5101 caagggctgg atattgtggt tggcgtggta gaaacccacg ggcggaaaga taccgccgca
     5161 atgctggaag ggctggctgt tctgccgtta aaacgccagg cgtaccgtgg gcggcacatc
     5221 agcgagtttg atctcgatgc cgccctcgcc cgccgtccgg cactgatctt aatggacgaa
     5281 ctggcgcaca gtaatgcgcc aggttctcgt catcccaaac gctggcagga catcgaagag
     5341 ctgctggaag ctggcattga tgttttcact accgtcaacg ttcaacatct ggaaagcctg
     5401 aatgatgtgg tcagcggcgt caccggaatt caggttcggg aaaccgtgcc cgatcctttt
     5461 tttgatgccg ccgacgacgt agtgctggtg gacttgcccc cggacgatct gcgccagcga
     5521 ctgaaagaag gcaaagtcta tattgccggg caggcggagc gcgccattga acattttttc
     5581 cgcaaaggca atctgatcgc cctgcgcgaa ctggcactgc gccgtactgc cgatcgcgta
     5641 gatgagcaaa tgcgcgcctg gcgggggcat cctggcgaag agaaagtctg gcacacgcgc
     5701 gacgcgatcc ttttatgcat cggccataac actggtagtg agaaactggt ccgcgcggcg
     5761 gcgcggctgg cgtcacggct gggtagcgtc tggcacgcgg tgtatgttga aacccctgcc
     5821 ctgcatcgct taccggagaa aaaacgtcgg gcaattctca gcgccttacg tctggcgcag
     5881 gagctgggcg cggagacggc aacactttct gatccagcgg aagagaaagc ggtagtgcgt
     5941 tacgcccgtg agcataatct cggcaagatt attctcggtc gcccagcctc gcgccgctgg
     6001 tggcgtcggg aaacgtttgc tgaccgactg gcgcgcatcg cccccgatct cgatcaggtg
     6061 ctggtcgcgc ttgatgaacc acccgcccgc acgattaaca acgcgccgga tagccgctct
     6121 tttaaagaca agtggcgggt acagattcag ggatgcgtgg ttgccgccgc gttatgcgcc
     6181 gttatcacct taattgccat gcagtggctg atggcgtttg atgccgccaa cctggtgatg
     6241 ctgtatctac ttggcgtggt ggtggtggcg ctattttatg gacgctggcc ttcggtggtt
     6301 gctaccgtca ttaatgtggt gagtttcgat ctctttttta tcgccccacg cggcacgctc
     6361 gccgtctctg atgtgcaata tctgctgacc ttcgcggtga tgttaaccgt cgggctggtg
     6421 atcgggaacc ttactgctgg cgtgcgttat caggcgcggg tagcccgtta ccgcgaacaa
     6481 cgcacacggc acttatatga aatgtccaaa gctctggctg tgggccgcag ttcgcaggat
     6541 atcgccgcca ccagcgaaca gtttattgcc tccacgtttc atgcccgtag ccaggtgttg
     6601 ctgcctgatg acaacggtaa attgcagccg ttaacacatc cgcaaggaat gacgccgtgg
     6661 gacgatgcca tcgcgcagtg gagttttgat aaaggtctgc ctgcgggcgc gggcaccgac
     6721 acgttacccg gtgtaccgta ccagattttg ccgctaaaaa gcggcgagaa aacctacgga
     6781 ctggtggtgg tggaaccggg gaatctgcgc cagttgatga tcccggaaca gcagcgcctg
     6841 ctggagacgt ttacgctgtt agtcgccaat gcgcttgagc ggctgacgct gaccgccagc
     6901 gaagaacagg cgcggatggc aagcgaacgt gaacagatcc gcaacgctct gctggcggcg
     6961 ctttcgcatg atttacgcac gccgctcacg gtgctgttcg gtcaggcaga aatcttaacg
     7021 ctcgatctgg caagcgaagg atcgccccac gcccgccagg ccagcgagat tcgtcagcat
     7081 gtgctgaaca cgacccgact ggtgaataat ctgctcgata tggcgcgaat tcagtccggc
     7141 ggctttaatt tgaagaaaga gtggttaacg ctggaagaag tggtcggcag cgcgctgcaa
     7201 atgctggaac cgggtttatc gtcgcctatc aatctttctc tgccagaacc gctgacctta
     7261 atccacgttg acgggccgct ctttgaacgg gtgctgatta atctgctgga gaacgcggtg
     7321 aaatacgcgg gtgcgcaggc cgaaattggt atcgatgccc acgttgaggg cgagaagcta
     7381 caactggatg tctgggataa cggccccggt cttccgccag gccaggagca gacgatattt
     7441 gataagtttg ctcgcgggaa taaagagtcg gcggtaccgg gtgtagggct tgggctggca
     7501 atttgtcggg cgattgtgga tgtacacggc ggcaccatta ccgcgttcaa ccgaccggaa
     7561 ggtggtgcct gttttcgtgt tacacttccc cagcaaaccg ctcctgaact tgaagaattt
     7621 catgaggata tgtgacaaac gttctgattg ttgaagatga acaggctatt cgtcgctttc
     7681 tgcgcacggc gctggaaggc gacggtatgc gcgtctttga ggccgaaacg ctgcaacgcg
     7741 gcttgctgga agcggctacc cgtaagccag atttgattat tctcgatctc ggcctgcccg
     7801 atggtgatgg gattgagttt atccgcgacc tgcgccagtg gagcgcggtg ccggtgattg
     7861 tgctttccgc acgtagcgaa gagagcgaca aaatcgccgc gctggatgcc ggagcggatg
     7921 attatctgag taagccgttt ggcattggcg aattgcaggc ccgtctgcgc gtcgctttac
     7981 gtcgccactc cgccaccgcc gcgcccgatc cgctggtgaa attttccgat gttaccgtcg
     8041 atttagcagc tcgtgtgatc catcggggcg aggaagaggt gcatctcacc ccgattgagt
     8101 tccgcctgct ggcagtgttg cttaataatg cgggaaaagt gctcacccag cgccagctcc
     8161 ttaaccaggt gtgggggcca aacgcggtcg aacacagtca ttatttgcgt atttatatgg
     8221 ggcatctgcg acaaaaactg gaacaggacc cggcccggcc tcgccatttc attactgaaa
     8281 ccggtattgg gtatcggttt atgctttgaa tattaatttc aatacatcct gccttttatt
     8341 aattaaagcc gtaataataa atacggcttt ttatcttaaa caacacacaa aaataacaat
     8401 tcaatatttt atattactga gtaaaaagct catcattaaa taaattaaga ttgatcattt
     8461 tttattgatc accttcacag ttcaaccgta tttcctggat agaatacctt caccttcatt
     8521 cacatcagga aaggtaaatt aaatggaaaa taacagccgc actatgcccc atataaggcg
     8581 gacaactcat attatgaagt ttgctcatcg caatagcttc gactttcact tcttcaatgc
     8641 ccgttagtct accgactaag ggcacttcag cgtacaggtc ttcctgactc tctgtattac
     8701 aggcatatgc ctgtattccg cagtgctcat tctctgttaa cccttgtatt atcagccacc
     8761 ggactgataa gggtttctgc attctgaaaa aagcaaactt ctgattaatt tatcagcggg
     8821 gtaactttgc tttttttccg gcacgatcga tttctcattc gagaaattga ggacctgcta
     8881 ttacccaaaa taaagagatg gaaaatgtca aaattaaaaa ttgcggttag tgattcttgc
     8941 ccggactgtt ttaccacgca gcgagaatgt atctatatta atgaaaacag taatatcgat
     9001 gtggcggcaa tagttttatc gctcaacgat gttacacgcg gaaaactcga tgaaatcgat
     9061 gccacgggtt atggcatccc ggtatttatt gctactgaaa atcaagaacg tgtacccgca
     9121 gagtatttgc cccgtatttc gggtgtcttt gagaattgcg aatcgcgacg agaattttat
     9181 ggtcgtcagt tagaaaccgc agccagccat tatgaaacgc aactgcgccc gcccttcttc
     9241 cgcgcactgg tcgattatgt caatcagggt aacagcgcgt ttgattgccc cggtcatcag
     9301 ggcggcgaat ttttccgtcg ccatcccgcg gggaatcagt ttgtggaata ctttggtgag
     9361 gcgctgttcc gtgctgactt gtgcaacgcc gacgtagcga tgggcgatct gctgattcac
     9421 gaaggcgcgc catgcattgc acagcaacat gcggcaaaag tgtttaatgc cgataaaaca
     9481 tacttcgttt taaatggcac ttcatcttct aacaaagtgg ttttaaacgc cctgctgaca
     9541 ccgggtgatc tggtgctgtt tgaccgcaat aaccacaaat ctaaccacca cggagcgttg
     9601 ctacaggctg gcgcgacacc ggtttatctg gaaaccgcac gtaacccgta tggttttatc
     9661 ggtggtattg acgctcactg ttttgaagaa agttacctgc gtgaactgat cgcggaattc
     9721 gcaccgcagc gggcaaaaga ggctcgtcct ttccgcctcg ccgttattca gctaggcact
     9781 tacgacggta cgatttataa cgcccgtcag gtggtggata aaattggtca tctgtgtgac
     9841 tacatcctct ttgactccgc atgggtcggc tatgagcagt ttatcccgat gatggcggac
     9901 tgttcaccac tgttgctgga tcttaatgag aacgatccgg gtattctggt tacgcaatct
     9961 gtgcataaac agcaggctgg tttttcccag acttcacaaa tccataaaaa agacagccat
    10021 atcaaagggc aacagcgtta tgtaccgcac aaacgcatga acaacgcctt tatgatgcat
    10081 gcctccacca gcccgttcta tccgctgttt gccgcactgg atatcaacgc caaaatgcat
    10141 gaaggtgtca gcggtcgtaa tatgtggatg gattgtgtgg taaatggcat taatgcccgc
    10201 aaactaatcc tcgataactg tcagcatatt cgtccattcg tacctgaact ggtggatggt
    10261 aaaccctggc agtcgtatga aacggcgcaa attgcggttg atctgcgctt cttccagttt
    10321 gtaccaggtg agcactggca ttcttttgaa ggctatgcag agaatcaata ctttgtcgat
    10381 ccgtgcaaac tgttgctgac aaccccaggt attgatgcac gtaacggcga atatgaagcg
    10441 ttcggtgtac ccgcgacgat tcttgctaac ttcctgcgcg aaaatggcgt agtgccggaa
    10501 aaatgcgatc ttaactccat cctctttctg ctgactccgg cagaagatat ggccaaactt
    10561 cagcaacttg ttgctctgct ggtacgcttc gaaaaactgc ttgagtccga cgcgccatta
    10621 tcagaagtac tgccttccat ctacaaacag catgaagagc gctacaccgg ttataccctg
    10681 cgtcagttgt gtcaggaaat gcatgatttg tatgcccgtc acaacgtgaa acaactgcaa
    10741 aaagagatgt tccgtaagga acacttccca cgcgtaagca tgaatccaca agaagccaac
    10801 tacgcctatt tacgcggtga agtggagctg gttcgtctgc cggatgcaga aggccgtatc
    10861 gctgccgaag gtgcgcttcc ttatcctccg ggtgtgctgt gtgttgttcc gggtgaaatc
    10921 tggggtggtg ctgttctgcg ttacttcagc gctctggaag aagggatcaa cctgctgcca
    10981 ggttttgcac cagagctgca gggtgtctat atcgaagaac atgatggtcg taagcaagtt
    11041 tggtgctatg tcatcaagcc tcgtgatgcg caaagcaccc tgttgaaagg ggaaaaatta
    11101 tgagtcaggc taaatcgaat aagatgggcg tcgttcagtt aaccatactg acgatggtca
    11161 acatgatggg ctccggtatc atcatgctgc cgacaaagct tgccgaagtt gggacaatct
    11221 caattatctc ctggctggtg acagccgtag gctcaatggc actggcatgg gcattcgcaa
    11281 aatgcggtat gttcagccgt aaatcaggcg gtatgggcgg ttatgcggag tatgctttcg
    11341 gtaaatccgg gaactttatg gcgaactaca cctacggtgt ctcgctgctg attgctaacg
    11401 tcgcgattgc tatttcggcg gttggttacg gcaccgaact gctcggcgcg agtttgtcgc
    11461 cagtgcagat tggtcttgcg accatcggag tgctgtggat ttgtaccgtg gctaactttg
    11521 gtggtgcgcg cattaccggg caaatcagta gcattactgt gtggggggtc attattccgg
    11581 tcgtcggtct gtgcattatc ggctggttct ggtttagccc gacgctatac gctaactcat
    11641 ggaacccgca ccatgtgccg ttctttagcg ctgtaggttc ttccatcgcc atgacgttgt
    11701 gggctttcct cggtctggag tcagcctgtg ccaacgccga agtagttgaa aacccggaac
    11761 gtaacgtgcc gattgcagtt cttggcggta cgttaggtgc ggcggtgatt tatatcgtct
    11821 ccaccaacgt gattgccggg attgtgccaa atatggagct ggcaaattca acggcaccat
    11881 ttggtctggc cttcgcgcag atgttcacgc cggaagtggg taaagtcatt atggcgctga
    11941 tggtgatgtc ctgctgcggt tcgctgcttg gctggcagtt caccattgcc caggtgttta
    12001 aatcttctgc cgatgaaggg tacttcccga aaattttctc ccgtgtgagc aaagtcgatg
    12061 caccagtaca gggaatgctg accattgtca tcatccagag cggattatca ctgatgacca
    12121 ttagcccgtc gctgaacagt cagttcaacg tgctggttaa cctggccgtg gtgaccaata
    12181 tcattccgta tattctgtca atggcggcat tagtcattat tcagaaggtc gctaatgtgc
    12241 ccccctcaaa agcgaaagtt gcaaactttg ttgcttttgt tggcgcgatg tacagctttt
    12301 atgcgctgta ctcatccggg gaagaagcca tgctatacgg ttccatcgtg accttcctcg
    12361 gttggacact gtatggtctg gtctcaccac gctttgaact gaaaaataaa cacggttaat
    12421 taagcaaagc aaaaaagggc gatcttccga ccgccctttt tcttatttga tatgtttacg
    12481 cgtttttcag aacttcgcta acaatctcaa ccgcttcttt ctcaatctgc ttgcgatgtt
    12541 cttcaccaag gaagctttcg cagtagatct tatatgcgtc ttccgtgcct gacggacgcg
    12601 cggcgaacca gccgttgtca gtcatcactt tcagaccgcc gatagaagcg ccattacccg
    12661 gtgccgccgt cagacgcgcg gtgatcgggt cacctgccag ggtgctggcg ctcaccattt
    12721 ccggagacag cttagacaac gccgcttttt gtgcggaagt cgctgctgcc tgcaaacggt
    12781 tatagctcgg cgcaccaaag cgttttgcca gttcgttata gtgttcctgc gggttcttac
    12841 cggtgacagc ggtgatttcc gccgccagca gacacataat gataccgtct ttatcggtgg
    12901 accacggcgt gccgtcgaaa cgcaggaagg aagcgcctgc actctcttcg ccgccaaagc
    12961 cgaagctgcc atcgaacaaa ccatcaacaa accatttgaa acctaccggt acttctacca
    13021 gcttacggcc caaatcattg accacacggt cgatcatcgc agatgaaacc agcgttttac
    13081 cgacagcaac atctttgccc cactgcggac gatgctggaa caggtagttg atcgccaccg
    13141 ccaggtagtg gttcggattc atcaaacctg ccggagtgac gataccgtga cggtcataat
    13201 ccgggtcgtt agcaaacgcc agatcgaact tatcacgcag tgccagcagg cccgccatcg
    13261 cacactcgga ggagcagtcc atacggatcg caccgtcttt atcgaggtgc ataaagcgga
    13321 aggtttgatc gacctgatcg ttaacgatgg tcaggttgag gttgtaatac tcgccaatgc
    13381 gcttccagta ttcgataccg gaaccaccca gcggatcaac gcccagcgtc aggcccgctt
    13441 tctgaatcgc cgccatatca acgatatcgg ccagcccttc cacaaacggc tgcaccagat
    13501 cctgctcttt cacatgaccg gatgccatcg cttcgtcgag ggagatacgc ttcacgcctt
    13561 tcaggccatc agccagcagt gcgttggctc tgtcttccac cactttagtg acgttggtat
    13621 cagccgggcc accatttggc ggattgtact tgataccacc atcttccggc gggttatggg
    13681 acggtgtaat cacgataccg tctgccagtg ggccaccttt tttattgtga accagaatgg
    13741 cattggaaat ggcaggtgtc ggagtgaagc cattgttttc ctgcacaatg acatcaacgc
    13801 cgttcgctgc cagcacttcc agtacggaaa tgaacgcagg ctcggacagg gcgtgagtat
    13861 ctttacccac atagcaaggg ccagtgatgc cgtttttcgc acgttcttca gcaattgcct
    13921 gagcgatcgc cagaatgtgc ggctcgttaa agctgtggcg cgctgcactg ccacggtgac
    13981 cggaagtacc gaatttcacc gcgtgctccg cattccctgc ttctggtttc agtacataat
    14041 attgcgccgt cagttgggcg acgttaatca aatcactctg ttgtgcaggt tggcctgcac
    14101 gattgtggat tgccattgct ttgtcctttg tctgcaacgt tttagatagt tccgcaaacc
    14161 ttctcaatca attccgccgg gaattgcatc gactgcatga tgtgttcgat catgctgcat
    14221 ttacggccgg tgttggtgtt ggtgatcacc caatacggcg taccaggcac atgtttcggc
    14281 ttggtctgat taccattttt cagcagcgtt tgttcatctg ccgcaaagta aacgcgtgta
    14341 cgaccgtgca acgattccgt tgcttcggca aacgcctggg cgtcaagaga atatagtgta
    14401 gacaacagca gcataaagcg attgaccgct cgcttttgct ctgcgtattc gtccgaaagc
    14461 agaagttcac gcattgcgcg aaccttgtct ttaatcgttt tgaccggctt cgcttcgacg
    14521 atagcaggtg acgcaacgcg aacctctttc gtcaccggag cagcaggctg tgatgcggcg
    14581 gaaaatttca acatacgccg taaaatgtcg gatgcgctct cgccgatatg cttagtgtgg
    14641 ctggcaatat agctgtagag ttcatcatca acttcaatcg ttttcatctt aatccagtgc
    14701 agtgtcttag ctgaatacaa gtcgccagga gtatatgaat aaatcccggc agcggatagc
    14761 gtcaagcctg gcttgcagaa aaactcggaa taaaccttat ttgttgcagc cctgtggcac
    14821 aatggtcaac ataataccct aacccgacaa cgcgaaaaaa agaactttgc catgaaattg
    14881 aatatccgcg cgcaaactgc acaaaaccag cacaataatt ctcccatcgt tcttgtccat
    14941 ggtctgtttg gcagcctcga caaccttggc gtactggctc gcgatctggt aaacgatcac
    15001 aatatcatcc aggttgatat gcgtaaccac ggtctttcac cgagagatcc ggtaatgaat
    15061 tacccggcga tggcgcagga tcttgttgat accctggatg cacagcagat cgacaaagca
    15121 acatttatcg gtcactccat gggcggtaaa gcggtaatgg ctcttactgc actagccccc
    15181 gatcgcattg acaagctggt ggcgattgat attgcgccgg tcgattatca cgttcgccgt
    15241 catgatgaga ttttcgcggc catcaacgca gtcagtgaat cagacgcaca aacacgccag
    15301 caagcagcgg caataatgcg ccagcatctt aatgaagaag gggtgattca gtttctgttg
    15361 aaatcttttg ttgacgggga gtggcgcttt aacgtgccag tattgtggga tcagtatccg
    15421 catattgtgg gttgggagaa aatcccggca tgggatcatc ctaccctgtt tatccctggc
    15481 ggcaattctc cgtatgttag cgagcagtac cgtgatgatt tactggcaca atttccacag
    15541 gctcgggcgc atgtgattgc aggcgcgggt cactgggtcc atgctgaaaa accggatgcg
    15601 gtattacgcg ctatccgtcg ctatctcaat gattaacgca actgattaaa aacccagcgt
    15661 ccgcgtcgct ttccgcgcgt ggacgttggc gtgactcatc gcgctgatgt attatggcgc
    15721 gctatccatc cgggcaggcg cctggctgat tgtttccccc gaagtcacca agatcatggc
    15781 caaagaacaa acggaccgta cgacattaga tctgttcgcg cacgagcgtc gaccgggacg
    15841 accgaaaact aatccgcttt cgcgcgatga gcagctgcgt attaataaac gcaaccaact
    15901 aaaacgcgac aaagtacgtg gccttaagcg tgtcgaactg aagctgaacg cggaagctgt
    15961 cgaggcactg aacgagctgg cggaatcgcg caatatgagc cgtagcgagc tgatcgaaga
    16021 gatgttaatg cagcaactgg cggcgctgcg tagtcagggc atcgtttaaa tttccacttt
    16081 catgtagcac agtgtgcagt cctgctcgtt tgctgattcc gcaagactgc ctgttctgct
    16141 atgattgcct ttatccgtgg gcaattttcc acccccattt caataagttt caagaggtta
    16201 tttcactcat ggctatcact ggcatctttt tcggcagcga caccggtaat accgaaaata
    16261 tcgcaaaaat gattcaaaaa cagcttggta aagacgttgc cgatgtccat gacattgcaa
    16321 aaagcagcaa agaagatctg gaagcatatg acattctgct gctgggcatc ccaacctggt
    16381 attacggcga agcgcagtgt gactgggatg acttcttccc gactctcgaa gagattgatt
    16441 tcaatggtaa actggttgcg ctgtttggtt gtggcgacca ggaagattac gcagaatact
    16501 tctgcgatgc attaggcacc atccgcgaca tcattgaacc gcgcggcgca actatcgttg
    16561 gtcactggcc aaccgcgggc taccacttcg aagcgtcaaa aggtctggct gatgatgacc
    16621 actttgtcgg tctggctatc gacgaagatc gtcagccgga actgactgct gaacgtgtag
    16681 aaaaatgggt taaacaaatt tctgaagagc tgcatctcga cgaaattctc aatgcctgat
    16741 gtgatgcggc gtagactcat gtctacgccg tattaataga taatgccaat caaaataatt
    16801 gctacaaatt tgtaactttt gctgttgtac ctgtacaatg tcccggtgtt caagtggcct
    16861 tgccgttgta aatgtaagct gtgccacgtt tttattaaca atatttgcca gggacttgtg
    16921 gttttcattt aggcgtggca attctataat gatacgcatt atctcaagag caaattctgt
    16981 cacttctcct aatgaagtga accgcttagt aacaggacag attccgcatg actgataaca
    17041 ataccgccct aaagaaagct ggcctgaaag taacgcttcc tcgtttaaaa atcctggaag
    17101 ttcttcagga gccggacaac catcacgtca gtgcggaaga tttatacaaa cgtctgatcg
    17161 atatgggtga agaaattggt ctggctacgg tatatcgcgt actgaaccag tttgacgatg
    17221 ctggtatcgt cacccgccac aattttgaag gcggtaaatc cgtatttgaa ctgacacagc
    17281 aacatcacca cgatcacctg atctgcctcg actgcggcaa ggttatcgaa tttagtgatg
    17341 attccatcga agcgcgtcag cgtgaaatcg ccgcaaaaca tggcattcgc ctgactaacc
    17401 acagtctcta tctttacggt cactgtgccg aaggcgattg ccgcgaagat gagcacgcgc
    17461 acgaaggcaa ataagccagc ctgaacgaga aaagccaacc tgcaggttgg cttttttata
    17521 caagggaaat aagaaaagcg agagttacag ctctcactta tttgttatta ctgcgaattt
    17581 cctgccagac cttatcacaa tcggctttca ccgcctgatc atttccggtt tgcgttgcct
    17641 gaatacactg ctgataatcc agtacgcgga cactttcctg ctcggcaaac tgctgatgtt
    17701 gtttctcttt cttcagcacg tttaacacgc tctggcaggc ttcaattttt tccggcgaac
    17761 cttgcgcggt gttgatacag gcgctgtaag cctctttcag acgactgtct tcctgcggtg
    17821 cggttgattg cgcacaagct accagccccg atgccatcat ggcgatgaga atcagttttt
    17881 tcatagcaaa ttcctcaaat gtgcggcagg cacaaaacct gccgcgtcgg gcatcagaag
    17941 atggtgaatg gtgcgattac cataaatttc acgtcgcgct catcctggaa gatgttaccg
    18001 taaccgccgc cccagcttgg gatgtcggag tggttgtcgt attcggtgaa gtgcagtttg
    18061 aacatcgtgc ctttggcgcg accgtcctga atggtgtaca ccgcatccag gctgtaggca
    18121 gactcttcaa tagtccggtt tttgtcgtag tacgcatccg gattgctctg ccaggtcgca
    18181 ggtttagcat cccatgcgta aacgtaggaa gcgccgatgg cgaagcctgg aagattccag
    18241 tttttcaggt catacatcgc accgaagaag accgcttttt cgccgttggc gttgaagtca
    18301 gaacggttat cccaccagat atccaggcga ccgttcgagg aagcgtaggt tggagtcata
    18361 cgttgcagga agtatccctg ctgaccgtcg gctttaaccc agctgccttc cagacgcaaa
    18421 tccactacat cagccgcccg gtaaccaaag gtcaacgctt gcagccaggc agtgccgtcg
    18481 taaatgtcgt tgacgctgcg atcgtcaact ttatcgcgcg taccgtagaa ctggtagctg
    18541 gtggttaacg ggctaccggc gatatcaaat ttgtagctgg ctttggcaaa atattgatct
    18601 atataccctt ccgcctgacc aaatgccgct tccagtacaa agttattttt gaagtcgtac
    18661 ttcgcaccga tggagtgcag ataatcaact ttggtagttt tatcgttctg ataaaactca
    18721 tccatttcga gatgccacgg tgctttgtat tcgttggtcc acatgtagga gaaactcaac
    18781 gcaccagcat cgccgtaatc aaaattcgcc ccggcttccg caccctgata agtacccggc
    18841 ataaagctcc agtgcggcgc taacagcgtc tgaccagttg gctgaatgta acctgccctc
    18901 gcccacaccg gaccgtattt aaatttggcc gctgctttat acaggcttat accgcttttg
    18961 tcgccggacc agtcttcgtc ataggcttta ttgctttttg aaaacgcgat ttcgttcggg
    19021 tgagagctgt caccgttttc cgccatttca atcgccgtaa acgcagcaat atcaaggccg
    19081 aacatatcag cagcataacc ggactgaaaa tcgaggttgg cattccaggt ggagtgagaa
    19141 aggttggttt tgtatttgtc gccgtcggta acatctttac ggtcgcgttc acgctgccag
    19201 taatagatac cgccggttaa ggttgaatcg tcgatgaatc cttcggcgcg agcctccggc
    19261 attgccataa agcccgacat tgctgtaaca ccggcgatag ccagcgccag cgtactacgt
    19321 ttgccactaa acgtacgcat gggttaatcc tctttgacgt ataaattgct gcaccaaagg
    19381 tgaacagcga aaaaaataaa aattaaaaaa gtagcgttga gaaaagtctc gctgactaca
    19441 ggaacagact atttttcgcg acgcgaatta tcaatctctt tctgtaacca gaatgtaaga
    19501 acatgagcaa ccgcacatat tttgatgatt tttgttacaa ggcttaagtt tgcagttgaa
    19561 aggcttgcag aagaataaaa gcgtgaattt ccagtaagat gaaaacggtt acattttatt
    19621 taattatggt ttgtgatttt aaatttgttt tatggataaa ttgcgaagcg gaattaattc
    19681 gcgaagaaaa gcagaaaaaa acgccgcaga gcgcggcgtt tgggattgtt tgcatatttc
    19741 ttgccggatg tggcgtaaat gcctcacccg gcctggtaga acttaaaact actcgcctac
    19801 tttcgcccag gtatcacgca gccctacggt gcggttaaat accggttttt cggcagtgga
    19861 atggcggctg tcgaggcaga aataaccttc acgctcaaac tggaatgctt tacccgctac
    19921 cgcgtctttc agcgacggtt cagcgaaacc ctgtttgatc accagcgatt ccgggttaat
    19981 caccgacagg aaatcatccg cagcacctgg gttaggcacg ctgaacagac ggtcatacaa
    20041 acggatttca accggcagcg catgtgccgc gctcacccag tgaataacac ctttcacttt
    20101 acgaccatct gccggatctt tgcttaaggt gtcggcgtca taagtacaga agatggtggt
    20161 gatattacct tcggcatctt tctcgacgcg ttctgcctta atcacgtaag cattacgcag
    20221 acgcacttct ttacccaaca ccagacgttt gtactgcttg ttagcttctt cgcggaaatc
    20281 ggcgcgatcg atccaaatct cacagctaaa cggcacctga cggctaccca tttccggttt
    20341 gttcggatgg ttcggcatgg tgaccatttc gccttcgccc tggtagtttt cgataaccag
    20401 tttcaccgga tcgataaccg ccattgcgcg cggggcattt tcgttgagat cttcacggat
    20461 gcaggattcc agtgacgcca tctcaatggt gttgtcctgc ttggtcacac caatgcgttt
    20521 gcagaactca cgaatagaag ccgcagtgta accacgacga cgcagaccag aaatggtcgg
    20581 catacgcggg tcatcccagc cttcaacgtg cttatcggtc accagcaggt tcagcttacg
    20641 cttggacatc acagtgtatt ccagattcag gcgcgagaat tcgtactggc gcgggtgaac
    20701 aggaatggtg atgttgtcca gtacccagtc gtacagacgg cggttgtcct ggaactcaag
    20761 cgtacacaga gagtgcgtaa taccttccag cgcatcgctg atgcagtggg tgaagtcgta
    20821 catcgggtag atgcaccact tgttgccagt ctggtggtgt tcagcaaatt taatgcggta
    20881 cagcaccgga tcgcgcatta cgataaatgg cgacgccatg tcgattttcg cacgcaggca
    20941 ggctttacct tcttcaaaac taccggtacg cattttttcg aacagcgcca ggttctcttc
    21001 aacgctgcgg tcgcggtacg ggctgttttt acccggctgc gtcagggtgc cgcggtattc
    21061 gcggatctgt tccggtgtca gttcatcaac gtacgccagg cctttattga tcagttcgat
    21121 cgcataggcg tggagctgat caaaataatc ggaggagtaa cggacgttac cagaccagtg
    21181 aaaacctaac cactcaacgt cgtttttgat cgactcaaca tactcgatat cttcttttac
    21241 cgggttagtg tcgtcgaaac gcaggttgca ctggccttta tagtcctggg cgatcccgaa
    21301 gttcaggcag atagatttcg catggccaat atgcagatag ccattcggct ccggcgggaa
    21361 acgggtgtgt actgtggtgt gcttaccact ggccagatct tcatcgatga tctgacgaat
    21421 aaagttagtc gggcgggctt ctgcctcact catcgtggat tcctcaaagc gtaaacaacg
    21481 tataacggcg tatgatctta taagcgggac aggctgacaa ctgttagtta gtgaaaatcg
    21541 aaaatctcac tgagattatg gggatgattg atgaaaaaaa aagcggcaga gaagatctcc
    21601 accgcttgtc attgttggat gcgacgctca agcgtcgcat caggcataaa gcagattact
    21661 ttttgatttc atacagcggt gtttgacccg ccacaacatg gccctgagct ttaatgatca
    21721 ggccgctgaa atcgtcgata ttgctgcaaa ccaccgggct aatcatcgag cgggcgttag
    21781 cgttcaggta atccagatcc atttccagaa tcggttgtcc tgcgcttacc tgcgcgccct
    21841 cttccaccag acgtttaaag cctttacctt ccagcgctac ggtgtcgata cccatatgga
    21901 cgacgatctc cgcgcctttt tcggtttcca gacagaacgc gtggttggtg ttgaagattt
    21961 tcacgattgt ccctgcggct ggtgatacga cgattttatc tgtcggtttc accgccacac
    22021 cgtcacccac cgctttgctg gcgaatgctt cgtcaggaac ctgatccagt gccacaacat
    22081 caccggtaat cggcgatacc agctccgcga tagacaccgc gtttggtaca gcctgcggtt
    22141 ttgctacagg cgcggcagtt gcaggcgcag tttcagctga cgcagcggct accggaccac
    22201 gggcaacgac tttcttcatc gcatcgccga tggattctgc tttcgcgccc acaatcacct
    22261 gaatagtctg tttgttcagt tttactaccc cagaagcccc cagacgttta cacatcacat
    22321 cgttgacgcg agctgagtca accacggtaa ggcgcagacg agtaatacag gcgtcaatgg
    22381 ctttcaggtt gtcagtaccg ccaaccgcag caatatagtt ggttgccagt tgattgagac
    22441 cttcttccgt gttgctgttg gcttcttcag taacgatctc gtcttcttta tcttcacggc
    22501 ccggcgtttt caggttgaac atgcggataa ccaaactgaa caccacgaag tagatagcga
    22561 agaagacaac acccatcacc agcagcatcc agacgttctg gctggcggcc ggcaggttat
    22621 acatcaacgc gtagtcgata gcccccgcag agaaggagaa gcccgcgtgg atacccagca
    22681 gcgttgccac aaacaggctg ataccggtca gcagtgcgtg caggaggtac agcagcggcg
    22741 caaggaacat gaacaggaat tccagcggct cagtcacacc ggtcaggaac gcagtaacag
    22801 caacagaaag cagcataccg ccaaccatcg gacgacgctc tttcggtgct gcaaagtaca
    22861 tcgccagcgc cgcacccggc agaccgaaca tcatgatcgg gaagaagccg gacatgaaca
    22921 tccccgcggt gccgtcaccg gcatagaagc ggttaatgtc gccgtggaaa accgtacccg
    22981 ccgcgttggt gaattcacca atctggaacc aggcgatggt gttcagtacc tgatgcagac
    23041 cggttgggat cagcagacgg ttgatgaaac caaagatacc ggaacccagc gcgcccgcag
    23101 aaacgatcca ctcgccgcct gcatggatag cgtgctgtac cggcggccag acgtaaccaa
    23161 aaatggccgc cagcaccagg cagaagaagc cggtggcaat cggcacaaag cgtttgccgc
    23221 cgaagaagct caggaagtcc ggcagtttaa tatcggacca acggttatag gctgcaccac
    23281 cgaccagacc ggtaatgata cccgccagta cacccatgtt aatttctggg ttgatggtca
    23341 ccatcgcttt ggttaacaca aagtaaccta ccgcacccgc cagtgccgcc gcacctgcgc
    23401 tgtctttcga ccagctggat gccacgccga tggcgaagat taatgcgagg ttatcaaaaa
    23461 tcgcaccgcc cgcctgggca ataaacgcaa cgttaagtaa atctggctga ccgaatcgca
    23521 gcaacagcgc cgccaccggc agcaccgcga tagggagctg taacgcccta cctagtcgct
    23581 ggaaaaaacc taaaatattc atcttattcc ccctacgaga accctatttg gctcgtttaa
    23641 agccgtattt ttattttgct gcaaattgta ctgccgatgt tctgtaatca gattgttaga
    23701 tcatctgcta cagagtgtgt gaaaatttaa ttcgtatcgc aaattaaacg cgtgtctttt
    23761 gtgagttttg tcaccaaata tcgttatcat cactcccttt tactggctaa accagaaaac
    23821 ttattttatc attcaaaaaa tcaggtcgga ttgacgcctg tctgcgcaaa tccaggttac
    23881 gcttaaagat gcctaatccg ccaacggctt acattttact tattgaggtg aataatgaga
    23941 ctgatccccc tgactaccgc tgaacaggtc ggcaaatggg ctgctcgcca tatcgtcaat
    24001 cgtatcaatg cgttcaaacc gactgccgat cgtccgtttg tactgggcct gccgactggc
    24061 ggcacgccga tgaccaccta taaagcgtta gtcgaaatgc ataaagcagg ccaggtcagc
    24121 tttaagcacg ttgtcacctt caacatggac gaatatgtcg gtctgccgaa agagcatccg
    24181 gaaagctact acagctttat gcaccgtaat ttcttcgatc acgttgatat tccagcagaa
    24241 aatatcaacc ttctcaacgg caacgccccg gatatcgacg ccgaatgccg ccagtatgaa
    24301 gaaaaaatcc gttcttacgg aaaaattcat ctgtttatgg gcggtgtagg taacgacggt
    24361 catattgcat ttaacgaacc ggcgtcttct ctggcttctc gtactcgtat caaaaccctg
    24421 actcatgaca ctcgcgtcgc gaactctcgt ttctttgata acgatgttaa tcaggtgcca
    24481 aaatatgccc tgactgtcgg tgttggtaca ttgctggatg ccgaagaagt gatgattctg
    24541 gtgctgggta gccagaaagc actggcactg caggccgccg ttgaaggttg cgtgaaccac
    24601 atgtggacca tcagctgtct gcaactgcat ccaaaagcga tcatggtgtg cgatgaacct
    24661 tccaccatgg agctgaaagt taagacttta agatatttca atgaattaga agcagaaaat
    24721 atcaaaggtc tgtaattatt atccctgccc tggctccttg ctcagggcaa tattttttaa
    24781 aatcgggggt cagaatgtat gcattaaccc agggccggat ctttaccggc cacgaatttc
    24841 ttgatgacca cgcggttgtt atcgctgatg gcctgattaa aagcgtctgt ccggtagcgg
    24901 aactgccgcc agagatcgaa caacgttcac tgaacggggc cattctctcc cccggtttta
    24961 tcgatgtgca gttaaacggc tgcggcggcg tgcagtttaa cgatactgct gaagcagtca
    25021 gcgtagaaac gctggaaatc atgcagaaag ccaatgagaa atcaggctgt actaactatt
    25081 tgccgacgct tatcaccacc agcgacgagc tgatgaaaca gggcgtgcgc gttatgcgcg
    25141 agtacctggc aaaacatccg aatcaggcgt taggtctgca tctggaaggt ccgtggctga
    25201 atctggtaaa aaaaggcacc cataatccga attttgtgcg taagcctgat gccgcgctgg
    25261 tcgatttcct gtgtgagaac gccgacgtga ttaccaaagt gactctggca ccggaaatgg
    25321 ttccagcgga agtcatcagc aaactggcaa atgccgggat tgtggtttct gctggtcact
    25381 ccaacgcgac gttgaaagaa gcgaaagccg gtttccgcgc ggggattacc tttgccaccc
    25441 atctgtacaa cgcgatgccg tatattaccg gtcgtgaacc gggcctggcg ggcgcgatcc
    25501 tcgacgaagc tgacatttat tgcggtatta ttgctgatgg cctgcatgtt gattacgcca
    25561 acattcgtaa cgctaagcgt ctgaaaggcg acaaattgtg tctggttacc gatgccaccg
    25621 cgccagcagg tgccaacatt gaacagttca tttttgcggg taaaacaata tactaccgta
    25681 atggactttg tgtggatgag aacggtacgt taagcggttc atccttaacc atgattgaag
    25741 gcgtgcgtaa tctggtcgaa cattgtggta tcgcactgga tgaagtgctg cgtatggcga
    25801 cgctctatcc ggcacgtgcg attggcgttg agaaacgtct cggcacgctc gccgcaggta
    25861 aagtagccaa cctgactgca ttcacacctg attttaaaat caccaggacc atcgttaacg
    25921 gtaacgaggt cgtaactcaa taagagaaag tatgacacca ggcggacaag ctcagatagg
    25981 taatgttgat ctcgtaaaac agcttaacag cgcggcggtt tatcgcctga ttgaccagta
    26041 cgggccaatc tcgcggattc agattgccga gcaaagtcag cttgcccccg ccagcgtaac
    26101 caaaattacg cgtcagctta tcgaacgcgg gctgatcaaa gaagttgatc agcaggcctc
    26161 caccgggggc cgccgcgcta tctccatcgt caccgaaacc cgcaatttcc acgcaatcgg
    26221 cgtacggctt ggtcgtcacg acgccaccat caccttgttt gatctcagca gcaaagtgct
    26281 ggcagaagaa cattacccgc tgccggaacg tacccagcag acgctggaac atgccctgct
    26341 gaatgccatt gctcagttta ttgatagcta ccagcgcaag ctacgcgagc tgatcgcgat
    26401 ttcggtgatc ctgccagggc ttgtagaccc ggacagcggc aaaattcatt atatgccgca
    26461 tattcaggta gaaaactggg ggctggttga agcactggaa gagcgtttta aagtgacctg
    26521 ttttgtcggc cacgatatcc gcagtctggc gctggcagag cactacttcg gtgcaagtca
    26581 ggattgcgaa gactccattc tggtgcgtgt ccatcgcgga accggggccg ggattatctc
    26641 taacgggcga atttttatcg ggcgcaacgg caacgtcggt gaaattggcc atattcaggt
    26701 cgaaccgctg ggtgaacgct gccactgcgg caactttggc tgcctggaaa ctatcgccgc
    26761 caatgctgcc attgaacaac gggtgttgaa tctgttaaag cagggctacc agagccgtgt
    26821 gccactggac gactgcacca tcaaaactat ctgcaaagcc gcgaacaaag gcgatagcct
    26881 ggcctcggaa gtgattgaat atgtcggtcg tcatctgggt aaaaccatcg ccattgctat
    26941 caacttattt aatccgcaaa aaattgttat tgctggagaa atcaccgaag ccgataaagt
    27001 gctgctccct gctattgaaa gctgcattaa tacccaggcg ctgaaggcgt ttcgtactaa
    27061 tctaccggtg gtacgttctg agttggatca ccgctcagca atcggcgctt ttgcgctggt
    27121 aaaacgcgcc atgctcaacg gtattttgct ccagcatttg ctggaaaatt aatgtgcttt
    27181 tatagtggcg cttattgttg tcaatattct gggtagtcca tgaccattaa aaatgtaatt
    27241 tgcgatatcg acggcgtgct gatgcacgat aacgtcgccg taccgggtgc agcggaattt
    27301 ttgcacggga ttatggataa aggcctgccg ctggtgttgc tgaccaacta tccttcgcag
    27361 accgggcaag atctggcgaa tcgctttgcc actgccggtg tcgatgtacc ggatagcgtg
    27421 ttttatacct ccgcgatggc gaccgccgat ttcctgcgtc gccaggaagg caagaaagcg
    27481 tatgtggtgg gcgaaggcgc actgattcat gaactgtaca aagccggttt cactattacc
    27541 gatgtgaacc ctgattttgt gattgttggc gaaacgcgtt cctacaactg ggacatgatg
    27601 cataaagcag cctatttcgt cgctaacggc gcacgcttta tcgccaccaa cccggacacc
    27661 cacgggcgcg gtttttatcc cgcctgtggc gcgttgtgtg cagggatcga gaaaatctcc
    27721 gggcgcaaac cgttctatgt tggaaagccg agtccgtgga tcatccgcgc ggcattaaac
    27781 aaaatgcagg cgcattcgga agaaacggtg attgtcggcg ataacttgcg taccgatatt
    27841 ctggcgggct tccaggccgg tctggagacg attctggtgc tttctggtgt ttcgtcactc
    27901 gacgatatcg acagtatgcc gttccgccca agttggattt acccatcggt cgctgaaatc
    27961 gacgttatct gaatagcaac cacgcctcag ggcgtggttt cttattttta cccatcatta
    28021 aaatcacccc attcaataaa acccgtcaaa aattatcgat ccatcaataa atatacccaa
    28081 aagccctccc ctttttcatc aatcagcaat cgccattacg ttttttattt ttccactgcc
    28141 aaatcgcttg cgcaccgtgc tgctttgcgg cattttttta acaagtaaac acaacacgca
    28201 gtaaacacca ggttaacgga gaaggttatg tgttcaattt ttggcgtatt cgatatcaaa
    28261 acagacgcag ttgagctgcg taagaaagca ctcgagctgt cacgcctgat gcgtcatcgt
    28321 ggcccggact ggtccggtat ttatgccagc gataacgcca tcctcgccca cgaacgtctg
    28381 tcaattgttg acgttaacgc gggggcgcaa cctctctaca accaacaaaa aactcatgtg
    28441 ctggcagtaa acggtgaaat ctacaaccac caggcattgc gcgctgaata tggcgatcgt
    28501 taccagttcc agaccgggtc tgactgtgaa gtgatcctcg cgctgtatca ggaaaaaggg
    28561 ccggaatttc tcgacgactt gcagggcatg tttgcctttg ccctgtacga cagcgaaaaa
    28621 gatgcatatc tgattggtcg cgaccatctg gggatcatcc cactgtatat gggctatgac
    28681 gaacacggtc agctgtatgt ggcctcagaa atgaaagccc tcgtgccagt ttgccgcacg
    28741 attaaagagt ttccggcggg gagctatttg tggagtcagg acggcgaaat ccgttcttat
    28801 tatcatcgcg actggttcga ctacgatgcg gtgaaagata acgtgaccga caaaaacgag
    28861 ctgcgtcagg cactggaaga ttccgttaaa agccatctga tgtctgatgt gccttacggt
    28921 gtgctgcttt ctggtggtct ggattcctca attatttccg ctatcaccaa gaaatacgcc
    28981 gcccgtcgcg tggaagatca ggaacgctct gaagcctggt ggccgcagtt gcactccttt
    29041 gctgtaggtc tgccgggctc accggatctg aaagcagccc aggaagtggc aaaccatctg
    29101 ggcacggtgc atcacgaaat tcacttcact gtacaggaag gtctggatgc catccgcgac
    29161 gtgatttacc acatcgaaac ttatgatgtg accactattc gcgcttcaac accgatgtat
    29221 ttaatgtcgc gtaagatcaa ggcgatgggc attaaaatgg tgctgtccgg tgaaggttct
    29281 gacgaagtgt ttggcggtta tctatacttc cacaaagcgc cgaacgctaa agagttgcat
    29341 gaagagacgg tgcgtaaact gctggccctg catatgtatg actgcgcgcg tgccaacaaa
    29401 gcgatgtcag cctggggcgt ggaagcacgc gttccgttcc tcgacaaaaa attccttgat
    29461 gtggcgatgc gcattaaccc acaggataaa atgtgcggta acggcaaaat ggaaaaacac
    29521 atcctgcgtg aatgttttga agcgtatctg cctgcaagcg tggcctggcg gcaaaaagag
    29581 cagttctccg atggcgtcgg ttacagttgg atcgacaccc tgaaagaagt ggcagcgcag
    29641 caggtgtctg atcaacagct ggaaactgcc cgcttccgct tcccgtacaa cacgccgacc
    29701 tcaaaagaag cgtatctgta ccgggagatc tttgaagaat tgttcccgct tccgagcgcc
    29761 gctgagtgcg tgccgggcgg tccttccgtc gcttgttctt ccgctaaagc gattgagtgg
    29821 gatgaagcgt tcaagaaaat ggacgatccg tctggtcgcg cggttggcgt tcaccagtcg
    29881 gcatataaat aagacgtgta atattgcccc ggagaaatcc ggggcaattt cagttactgg
    29941 ggcgcttgtt gattctgata tgcagtaagg aaatgttccg ggtttgccac atcgctgttt
    30001 gacaactcat tactgtaggc cagtatttca tagattaatt tcccaccact attgttgaat
    30061 tccgaatttc atcctgtatg acaaaagaat ggctgtaggg tggggaaaaa acacaaagta
    30121 aacaataatt gacgaatata gcgccatgct gttcgcaacc taaccaaaca gtcactttcg
    30181 agcaattttc cttgaaaaag aggttgacgc tgcaaggctc tatacgcata atgcgccccg
    30241 caacgccgat aaggtatcgc gaaaaaaaag atggctacgt agctcagttg gttagagcac
    30301 atcactcata atgatggggt cacaggttcg aatcccgtcg tagccaccat cttttttgcg
    30361 ggagtggcga aattggtaga cgcaccagat ttaggttctg gcgccgcaag gtgtgcgagt
    30421 tcaagtctcg cctcccgcac cattcaccag aaagcgttgt acggatgggg tatcgccaag
    30481 cggtaaggca ccggtttttg ataccggcat tccctggttc gaatccaggt accccagcca
    30541 tattcttcga ataagcggtt caccgcccgg ttattggggt atcgccaagc ggtaaggcac
    30601 cggtttttga taccggcatt ccctggttcg aatccaggta ccccagccat cgaagaaaca
    30661 atctggctac gtagctcagt tggttagagc acatcactca taatgatggg gtcacaggtt
    30721 cgaatcccgt cgtagccacc aaattctgaa tgtgtcgaat atgttcggca aattcaaaaa
    30781 ccaatttgtt ggggtatcgc caagcggtaa ggcaccggat tctgattccg gcattccgag
    30841 gttcgaatcc tcgtacccca gccaatttat tcaagacgct taccttgtaa gtgcacccag
    30901 ttggggtatc gccaagcggt aaggcaccgg attctgattc cggcattccg aggttcgaat
    30961 cctcgtaccc cagccacatt aaaaaagctc gcctcggcga gctttttgct tttctgcgta
    31021 tattcaatgc cgaatgcgat gttgactcgt cttatccttc aaagtcggat gcgacgctgc
    31081 cgcgtcttat ccgacctacg gttggcatgc atccagcaat gttgtgaggc tacaacccta
    31141 acgcatattt cagcgcctta cgtttcaaca cgccagcacg ctccgccgcc atcaaaccaa
    31201 gattacgcac aaaacgcagc ggtggcagat tattgctgaa tccggcataa aacagatcca
    31261 taccactttg catcataaag ttatccgcca tacgccgcat ctggtaacgc ttgagcacag
    31321 gataactggc ccacgcttcg ccgtagctgc gggcgttcac cagaacgtca atcagggcat
    31381 cgacatcacg ataaccaaga ttcaccccct gccccgccag cggatggatg gtatgcgcgg
    31441 catcgcccac cagagcaagc cctggttgca catactgcaa cgcatggcga cgtatcagcg
    31501 gaaacgcacc agcggcaagc ggtgtcacgt aacccagacg cgacgggaaa tgcttcgcga
    31561 tttccgcctg tagctgcgcc atattcatat tctgcaactg gcgaatgcgc gccggagagt
    31621 cataccacac cagcgatgcc cagttatcaa acaacggcag aaacgcacgc ggtccgtccg
    31681 gggtaaattg ctgccaggtg ctgtcgccgg gatcgttctc acactgtacg ctaatcaaca
    31741 tacacgactg cgcatactgc caggcatgaa cgccaattcc cgccatctgt cgcacctgcg
    31801 aatttgcgcc gtcggcacca atcaccagct tcgcgaggat cgtttcaccg cccttcagtt
    31861 ccagctcctg aagatcatta tggcgatgca gtgcaatcag cgaggttggc acacgtaacg
    31921 ttactttcgg atgcgcttcc agcgcctgcc acaacgcctg ttgcaggaca gtgttttcca
    31981 ccatgtagcc aagcaacggt agcttaagtt cagcggcgtc aaacaccaca tgcgccgttt
    32041 cccactccca cgtttccagt ctgcggtaag gatggcaacg catagcctgt actgcatccc
    32101 agacccctaa ccctttaagc aatgataccg aagccgcact aatcgccgag atccgcacgt
    32161 ccggctggct gtcagcgaca aacggtgcag gttgggcatg ttcgataacc attaccgaaa
    32221 atccgtgctg agccagtccc agcgccagtg cgccgccgac cattcctccg ccgacaatgg
    32281 caatttccgt tggttgattt gtcatggtca atcatcctgt tagcaatatg ctttaagttt
    32341 acaggatttt tacccccaag ggctgataac gctcatactg gtcacaacgg cagcaaagca
    32401 ttacactatg cgccctgcat tcctggctac tatttcgcaa gagcaagtcg atgaccaaaa
    32461 aactccatat taaaacctgg ggctgtcaga tgaacgagta cgattcatcg aagatggccg
    32521 atctgctgga tgccacccac ggctatcaac tgaccgacgt ggcggaagaa gcggatgtgc
    32581 tgctgctgaa cacctgctca atccgcgaga aggctcagga aaaagtcttc catcagttgg
    32641 gtcgctggaa actgttaaaa gagaagaatc cagacctgat tatcggcgtc ggcggctgcg
    32701 tggcatcgca agaaggcgag cacattcgcc agcgcgccca ctatgtcgat attatttttg
    32761 ggccgcaaac gctgcaccgt ctgccggaga tgatcaactc cgtgcgcggc gaccgcagcc
    32821 cggttgtaga tatcagcttc ccggaaatcg agaagtttga ccgtctgccg gaaccgcgcg
    32881 ccgaagggcc gaccgcgttt gtctccatca tggaaggctg caataaatat tgcacctact
    32941 gcgtagtgcc ttacacccgt ggtgaagagg taagccgtcc gtccgacgat attctgtttg
    33001 agattgccca gcttgctgct cagggcgtgc gtgaagttaa cctgctcggc cagaacgtga
    33061 acgcctggcg cggtgagaac tacgacggca ccaccggatc gtttgccgat ctgctgcgtc
    33121 tggttgcggc gatcgacggg atcgatcgta ttcgctttac caccagccat ccgatcgaat
    33181 tcaccgacga tatcatcgaa gtgtaccgcg acacgccgga gctggtgagc ttcctgcatc
    33241 tgccggtaca gagcggttcc gatcgcattc tgaacctgat gggacgtacc catacagcgc
    33301 tggagtacaa agcgatcatc cgtaagctgc gtgcggcgcg tccggatatt cagatcagtt
    33361 ctgacttcat cgttggcttc cctggcgaaa ccaccgaaga cttcgagaaa acgatgaagc
    33421 tgattgccga cgtcaatttc gacatgagct acagctttat cttctccgct cgtccaggta
    33481 caccagccgc cgatatggtt gatgatgttc cggaagaaga gaagaagcag cgcctgtata
    33541 ttctacaaga acgtatcaat cagcaagcaa tggcatggag ccgccggatg ctcggtacca
    33601 cccagcgtat tctggtagaa ggtacatcgc gtaagagcat catggagctt tccggccgta
    33661 ctgaaaataa ccgcgtcgtc aacttcgaag gtacgccgga tatgatcggt aaattcgtcg
    33721 atgtagaaat taccgacgtc tacccgaact ctctgcgcgg taaagtggtg cgaactgaag
    33781 atgaaatggg tctgcgcgtg gcagaaacac cggaatcggt gattgcccgt acccgcaaag
    33841 aaaacgacct tggcgtgggt tattatcagc cgtaattctc aggcccgccg ttccggtggg
    33901 ccttgttttt tctctcttta tccccatctt tatcgcaatg cttgcgcctt tttcctgtgg
    33961 cgtgaataat ttccttatag gccggtgaat gttcagcttc cggcggcata cagataagag
    34021 gaacggtttg aacatagaca ctcgcgaaat caccctggag ccagcagaca atgcgcgtct
    34081 gttgagcctg tgcggcccgt ttgatgacaa catcaaacaa ctcgaacgcc gtctcggcat
    34141 cgaaatcaat cgccgcgata accactttaa actgaccggc cgtccgattt gcgtcaccgc
    34201 tgcggcagac attctgcgta gcctgtatgt cgatactgcc ccgatgcgcg gtcagattca
    34261 ggatatcgaa ccggaacaga tccaccttgc gattaaagaa gcgcgggtac tggagcaaag
    34321 cgcggagagc gtgccggagt acggcaaagc ggtcaatatc aaaaccaaac gcggcgtaat
    34381 taagccgcgc acgccaaacc aggcgcagta catcgccaat attctcgacc atgacattac
    34441 cttcggcgtt ggcccggcgg gtacgggtaa aacctacctg gcagtggctg cggcagttga
    34501 tgccctggag cgtcaggaaa ttcgccgtat tctgctgact cgtccggcag tagaagccgg
    34561 tgagaaactg ggcttcctgc ctggcgattt aagccagaaa gtagacccgt atctgcgccc
    34621 gctgtacgac gcgctgtttg aaatgctggg ctttgagaaa gtcgagaaac tgattgagcg
    34681 caacgttatt gaagtcgcac cgctggccta tatgcgtggt cgtacgctga acgacgcgtt
    34741 tatcattctt gatgagagcc agaacaccac catcgaacag atgaagatgt tcctgacccg
    34801 tatcggtttt aactcaaaag cggttatcac cggcgacgtc acacagatcg acctgccgcg
    34861 taataccaag tctggtttgc gtcatgccat cgaagtactg gctgatgtcg aagagatcag
    34921 ctttaacttc ttccacagcg aagacgtggt tcgtcacccg gtggtggcgc gtatcgttaa
    34981 cgcctatgaa gcctgggaag aagccgaaca aaaacgtaaa gcggcgctgg cagcagaacg
    35041 caagcgcgaa gaacaggaac aaaaatgagt caggtgatcc tcgatttaca actggcatgt
    35101 gaagataatt ccgggttacc ggaagagagc cagtttcaga catggctgaa tgcggtgatc
    35161 ccgcagtttc aggaagaatc ggaagtgact attcgcgtgg tcgataccgc cgaaagccac
    35221 agtctgaatc tgacctatcg cggtaaggat aagccgacca acgtgctctc cttcccgttt
    35281 gaagtaccgc ctggcatgga aatgtcgcta ctgggcgatc tggttatctg ccgtcaggtg
    35341 gttgagaagg aagctcagga gcaaggcaaa ccactggagg cgcactgggc gcatatggtg
    35401 gtgcacggca gtctgcattt gttaggttac gatcacatcg aagatgacga agcagaagaa
    35461 atggaagcca tcgaaacaga gattatgctt gctctgggct atgaggatcc gtacattgcc
    35521 gagaaagaat aatttgccag gcatttgact ggcagacgtc cccgccgttg gtgattaacg
    35581 acggcgtttt gattaactaa catgagaacc catacgacgc catgagcgac gacaattcac
    35641 acagtagtga cacgataagc aacaagaagg gatttttctc cctgttactc agccaacttt
    35701 tccacggtga accgaaaaac cgtgacgaac tgctggcgct gatccgtgat tccgggcaga
    35761 acgaccttat cgacgaagat acgcgcgata tgctcgaagg ggtgatggac atcgcagacc
    35821 aacgcgttcg cgacatcatg atcccccgct cccagatgat taccctgaaa cgcaaccaga
    35881 cgctggacga atgccttgat gtcatcatcg agtccgccca ctcacgtttc ccggtgatta
    35941 gcgaagacaa agatcacatt gaagggattc tgatggcgaa agacttgctg ccgtttatgc
    36001 gcagcgatgc tgaagccttc agcatggaca aagtgttacg tcaggcggtt gtcgttcctg
    36061 aaagtaagcg cgtagaccgg atgctgaaag agtttcgctc tcagcgttac cacatggcga
    36121 tcgtcattga cgaattcggt ggggtttccg gtctggtgac cattgaagac atcctggaac
    36181 tgattgttgg tgagattgaa gacgagtatg acgaagaaga tgatatcgac ttccgtcagc
    36241 tgagtcgtca tacctggacc gtgcgcgcac tggcttctat tgaagacttc aacgaagcgt
    36301 tcggcaccca ctttagcgat gaagaggttg acactatcgg tggtctggtg atgcaggcat
    36361 ttgggcatct tccggcgcgt ggcgaaacca tcgacatcga cggttaccag ttcaaagtgg
    36421 cgatggccga cagtcggcgt attattcagg ttcatgtcaa aatcccggat gactcacccc
    36481 agccgaagct ggatgaataa aaccgaaact ggatagataa ctacatggct tttgcctcat
    36541 taattgaacg ccagcgcatt cgcctgctgc tggcgttatt attcggtgcc tgcggaacgc
    36601 tggccttctc tccttacgac gtctggcctg cggcgattat ttcgctgatg gggcttcagg
    36661 cgttgacctt taaccgccgt ccactccagt ctgccgctat tggcttttgc tggggatttg
    36721 gcctctttgg cagcggtatt aactgggtct atgtcagcat cgcgaccttt ggcggaatgc
    36781 ctggcccggt taacatcttc ctggtggtac tgctggcggc gtatttgtcg ctgtataccg
    36841 gactgtttgc tggcgtgctg tcgcgtctgt ggccgaaaac cacctggctg cgcgtggcga
    36901 ttgccgcccc tgctctctgg caagtgaccg agtttctgcg cggttgggta ctaacaggct
    36961 tcccgtggtt acagttcggc tatagccaga ttgatggccc gttaaaaggg ctggcaccga
    37021 taatgggcgt ggaagccatt aacttcctgc tgatgatggt tagcggcctg ctggcactgg
    37081 cgttggtcaa acgcaactgg cgtccgctgg tggtggccgt cgtgctgttt gcccttccct
    37141 tcccgctgcg ttacatccag tggtttaccc cgcaaccgga aaaaactatt caggtttcga
    37201 tggttcaggg cgatattccg caatcgctga aatgggacgg agaccagcta cttaatacgc
    37261 tgaagatata ctacaacgca acggcaccgc tgatgggcaa atcatcgttg attatctggc
    37321 cggagtcggc gataaccgat ctggaaatta atcagcaacc gttcctcaaa gctctggacg
    37381 gtgagttgcg cgataaaggg agctcgctgg tcaccgggat tgtcgacgcg cgtctcaata
    37441 aacagaaccg ctacgatacc tacaacacca tcatcacact gggtaaaggc gcaccgtaca
    37501 gctatgaatc agccgatcgc tataacaaaa accatctggt gccgtttggc gagtttgtcc
    37561 cactggagtc gatactgcgt ccgttagcac cgttctttga tttgccgatg tcttcattca
    37621 gccgcggacc atatattcag ccaccgctgt cgttgaatgg cattcaactt actgcggcca
    37681 tttgctacga aatcattctc ggcgagcaag tgcgcgataa cttccgcccg gataccgact
    37741 atctgctgac tatctccaac gatgcgtggt ttggtaagtc tattggtcca tggcagcact
    37801 tccagatggc gcgaatgcgt gcgctggagc tggcgcgccc actgttgcgc agcaccaaca
    37861 acggcattac ggcggtgatt ggcccgcagg gtgagattca ggcgatgatc ccgcagttca
    37921 cccgcgaggt gttaaccact aacgtgacgc cgactaccgg actcacaccg tacgcacgta
    37981 ccggcaactg gccgctgtgg gtgctgacgg cactgtttgg ttttgcagct gtgctgatga
    38041 gtctgcgagc acgtaaacat taatgtgcct gatgtatccg gcatgaacaa aacatacttt
    38101 ctcattgagt tgtggggcat gattatcatg cccttttttc ttacggttca gggatacaag
    38161 ccacatcgcc atattgggga tgccgaataa ttgcctgcgg atcaagcgag cgcgtcctgt
    38221 ccatttggat taacctgttc ttcgccataa caaatatctt tctaccatcc gctgaaaaac
    38281 gaaggtgatt aacgtagctg gcaggataaa cgccaataat ctgcccggca ttaatattga
    38341 gcaggatgac ataaccttgc aggccatatg acaccaacgc taaatattga ccgtccgggc
    38401 taataccctc aacggaataa acgctcctgc tacaccattg cgtcataatg ctggcttgtt
    38461 gatcccctac gcttatacgt gaaatcccct ggctatcggc aaaaataatc tcgccatttt
    38521 ttggtcggaa tcccatcgca gaaacgatac cacgcgcatc acctgtggga aagtcttctt
    38581 tctgatgcgt gatggtattg atgcgtacaa tgtcgcctct gaccataccg ggtatcaaca
    38641 atgttttttc atccggggaa agtgccataa cccaggcgcc tggtttaccg ctcgtccccg
    38701 tcccgtcgta tttctccagc gggatatccg tcacatgctg ataagtcacc agatcaatgg
    38761 ctgacacact attactggtg cgattcatca cccaggcttt tgtgccatca cgcgagagga
    38821 taaggctgtc aggatgggcg ccggtggtaa tcgactgaat aatctccccc gtcgccacgt
    38881 taatgaccga tactgcattc gcttcaaatg aagtcgcata aatcaacttt tcatccaggc
    38941 ttaatgccag cgatctgacg ccctgatctt tacagccacc acaatgtttg actgactttt
    39001 ctttcgctat gggcgctgta aaggtatgca ggattttctt ttgtccgagg tcgaaaacaa
    39061 caatgctgtt ggtgttttca attgcggcat acaacgtatt accatcccga ctgatcgcca
    39121 tatcatggat atcggcaatc tcagcgagta aatcaccact ggcctgctca cgacttaccg
    39181 gagcggataa tgtttccctg agggcgttgc gctcctccac taactgctgc tttttttgtc
    39241 gtcgctcttg ttcaatctcc tgcggataaa acgcttcacg caggctttgc ctgacctgta
    39301 aaactgattc atcaatctgc tgctgcacaa accagatagt cccggtcaga agaagagtga
    39361 ccgcaacaat aatcccgcct tccgtaacga gccgattttg cccaactaaa cgcgcactca
    39421 gccaacccaa cgcgcccccc gcaatcaggc cgccaatgtg gcaagcgtta tctattcctg
    39481 cttgccgggc accatacaac agtgtcagcg ccaccatacc caacagagaa aatactcgcc
    39541 gctggttttt atggtgtgta cccgcacctg aagaaagctg tgttgctatc gcagcgcccg
    39601 ctattcccat aattgccccg gatgccccaa cgctgatgac tatctgctca ctttcgcgta
    39661 acgcataaca ggcgctgatc agcccaccaa agatgccgga gaataaccag ataatcagga
    39721 gtcgccattt ccccagaata cgttccaccg ctccccccac caccaggagt gcaaacatat
    39781 tcatcagcaa atgagaaaca ctgctgtgca gaaagacact gctcaataag cgccaggatt
    39841 cacccgacaa cgtgagagaa gcaacgttag caccgagatg gataagtgcg gattgatcga
    39901 cttcttcata actcatccat tgtgcgaggt aacaaaatat cccaacacag agcgcaaaaa
    39961 ggataagggt tattgcgggt aattgaacat ttaacggctt aaccgaagat gcagacataa
    40021 ggtgattctt ccttgaaaag acaggtaaaa agcatttttc ccgtgtgcag ttatcatccc
    40081 ggcaaacgtc ccatgccgaa aagataaccc tgtccgaaat atgactattt cagcctaaca
    40141 ttacaatcgt aattaattca cctataagta ttttttggca tgtaagttgc tttatttact
    40201 gtgtaagcaa tgaaatcagc cgcgacgcaa ccgggttgca ccaccacaaa tcagcggtag
    40261 caccaaaaag gtgcgattta agaattttgc ttcggaatgg tgcggcgcat ctcgcaaaaa
    40321 caaacaataa aacagcaata tctttacatt tagccaaact aaatgttacc taacaagcac
    40381 aacactgcac aataaagttg cagatgataa caacacaaac actcacaacg ggtatccatg
    40441 cgttcttaac gcagaagata aaggagttgg atatgcaatt acgtaaacct gccacagcaa
    40501 tcctggccct ggcgctttcc gcaggactgg cacaggcaga tgacgccgcc ccggcagcag
    40561 gcagtacgct ggacaaaatc gccaaaaacg gcgtgattgt cgtcggtcac cgtgaatctt
    40621 cagtaccttt ctcttattac gacaatcagc aaaaagtggt gggctactcg caggattact
    40681 ccaacgccat tgttgaagca gtgaaaaaga aactcaacaa accagactta caggtgaaat
    40741 tgattccgat cacctcacaa aaccgtattc cactgctgca aaacggcact ttcgattttg
    40801 aatgcggttc taccaccaac aatgccgaac gccaaaaaca ggcggctttc tctgacacta
    40861 ttttcgtggt cggtacgcgc ctgctgacca aaaagggcgg cgatatcaaa gattttgccg
    40921 acctgaaagg caaagccgta gtcgtcactt ccggcaccac ctctgaagtt ttgctcaaca
    40981 aactgaatga agagcaaaaa atgaatatgc gcatcatcag cgccaaagat cacggtgact
    41041 ctttccgtac cctggaaagc ggtcgtgccg ttgcctttat gatggatgat gctctgctgg
    41101 ccggtgaacg tgcgaaagcg aagaaaccag acaactggga aatcgtcggc aagccgcagt
    41161 ctcaggaagc ctatggttgc atgttgcgta aagacgatcc gcagttcaaa aagctgatgg
    41221 atgataccat cgctcaggtg cagacctccg gtgaagcgga aaaatggttt gataagtggt
    41281 tcaaaaatcc aattccgccg aaaaacctga acatgaattt cgaactgtca gacgaaatga
    41341 aagcactgtt caaagaaccg aatgacaagg cactgaacta attacaagaa ccaggggcgg
    41401 aaattccagc cctctcgatt gttacgtagc acggacagac tatacgcctg gtggtcgttc
    41461 cccatcgggc ctgaaaaccg caatacgctg ggtaacaatc ttcgagggta gcagttaacg
    41521 ctgctaccct cttttttctg gagtagattt atgtctatag actggaactg gggtattttt
    41581 ttacaacaag ccccgttcgg caacaccacc tatcttggtt ggatctggag tggttttcag
    41641 gtgacgatcg ctttgtcgat ctgcgcctgg attatcgctt tcctcgtcgg ttcatttttc
    41701 ggcattttac gtaccgttcc aaaccgtttt ctctccggtc tgggtacgtt gtatgtcgaa
    41761 ctgttccgca acgtgccgtt gattgtgcaa ttctttacct ggtacctggt gatcccggag
    41821 ctgctgccag agaaaatcgg catgtggttt aaggcagagc tggatcccaa tattcagttt
    41881 ttcctttcat ccatgctctg cctggggctg tttaccgccg cccgtgtttg cgaacaggta
    41941 cgcgccgcta ttcagtcgct gccgcgtggg caaaaaaatg ccgcgctggc gatgggtctg
    42001 acgctaccgc aggcttaccg ctacgtactg ctgcctaatg cttatcgcgt tatcgtcccg
    42061 ccgatgacct cagagatgat gaacctggtg aaaaactccg ccatcgcctc gactatcggt
    42121 ctggtggata tggcggcgca ggcgggtaaa ttgctcgatt actcggccca cgcctgggag
    42181 tcgtttaccg ccatcacgct ggcgtatgta ttgattaacg cttttatcat gctggtgatg
    42241 acgctggttg aacgtaaagt tcgtctgccc ggcaacatgg ggggcaaata atgtacgagt
    42301 ttgactggag ttccattgtt ccttccctgc catatctgct cgacgggctg gtgatcacgc
    42361 tgaaaatcac cgtcacggca gtggtgattg gtattttgtg gggcaccatg ctggcggtga
    42421 tgcgcttgtc cagctttgcg cctgttgcgt ggtttgccaa agcctatgtt aacgtattcc
    42481 gctcgattcc tttagtcatg gttttgctgt ggttttacct gattgtgccg ggatttctgc
    42541 aaaacgtgct gggattatcg ccaaaaaatg atatccgttt aatttcggca atggtggcgt
    42601 tttcgatgtt tgaagcggcc tactattcag agattatccg tgccggtatt caaagtattt
    42661 cccgtggtca gtcgagcgcc gcgctggcgt tggggatgac tcactggcag tcgatgaaac
    42721 tgattattct gccgcaggct ttccgcgcga tggtgccgct gctgctcact cagggcatcg
    42781 tactgttcca ggatacctcg ctggtgtatg tgttatctct ggccgatttc ttccgtaccg
    42841 cttcaaccat tggtgagcgt gatggtacgc aggttgagat gatcctgttt gccggatttg
    42901 tttatttcgt tattagcctt agcgcgtcgt tgttggtcag ctacttgaaa agaaggacag
    42961 cataatgatt accctgaaaa atgtttcaaa atggtatggt cactttcagg tgctgaccga
    43021 ctgctcaacc gaagtgaaaa aaggcgaagt ggtggtggtt tgcggcccgt ctggttccgg
    43081 caaatcaacg ctgattaaaa ccgtcaacgg cctcgaaccg gtgcagcaag gtgaaatcac
    43141 cgtcgatggc atcgtggtta acgacaagaa aaccgatctg gcaaagctgc gttcccgcgt
    43201 cgggatggta ttccagcatt tcgagctgtt ccctcatctg tcgattatcg aaaacctgac
    43261 cctggcgcag gtgaaagtgc ttaaacgcga taaagcgccg gctcgtgaaa aagcattgaa
    43321 attgctggag cgcgtcgggc tttccgcaca tgccaataag tttccggcgc agctttccgg
    43381 cggtcagcag cagcgtgtgg cgatcgctcg cgcattgtgt atggatccta ttgccatgct
    43441 gttcgacgaa ccgacatcgg cgctggatcc ggagatgatc aacgaagtgc tggacgtgat
    43501 ggtggaactg gcgaacgaag gaatgaccat gatggtggtg acccacgaaa tgggctttgc
    43561 ccgtaaagtg gcgaatcggg tgatctttat ggacgagggt aaaattgtcg aagactcgcc
    43621 gaaagacgct ttcttcgatg atccgaaatc ggaccgcgca aaagacttcc tcgcgaaaat
    43681 cctgcattaa tcactctggc gcgcataccc aagtgcgcgc catctcacgc tttatcgttt
    43741 cgctcctcgt atctgttaca tcctagcggt acccttgtcg caaagaagca cacaacaagg
    43801 agcaacaatg gcactgccaa ttctgttaga ttgcgaccca ggtcatgacg acgctatcgc
    43861 aatagttctt gccctcgcct caccagagct tgatgtcaaa gcaattacgt cttccgccgg
    43921 aaaccagaca ccagaaaaaa ccttacgcaa tgttctgcgt atgctgacct tgcttaatcg
    43981 caccgatatt ccggtagcag gcggcgcggt aaaaccgtta atgcgtaatt tgattattgc
    44041 ggacaatgtg cacggcgaaa gcggtctcga cggcccggca ttaccggaac cgacgttcgc
    44101 accgcaaaac tgtacggcgg tggagctaat ggcgaaaacg ctgtgtgaaa gtgaggaacc
    44161 tatcaccatt gtttcaaccg gaccgcaaac taacgttgcc ttgctgctca atagccaccc
    44221 ggaactgcat agcaaaattg cccgtatcgt gattatgggc ggcgcaatgg ggcttggtaa
    44281 ctggacgcct gcggctgaat ttaatattta cgttgacccg gaagcggcag aaattgtctt
    44341 ccagtcagga attccggtgg tgatggccgg tctggatgtt actcacaaag cacaaatcca
    44401 cgttgaagac actgagcgtt tccgcgcgat tggcaaccct gtttcaacca ttgttgccga
    44461 actgctggat ttcttcctcg aatatcataa agacgaaaaa tggggctttg tcggcgcacc
    44521 actgcatgac ccatgcacca tcgcctggct gttgaaaccg gagttgttta ccactgttga
    44581 gcgctgggtt ggcgtggaaa cacaagggaa atatacccag ggtatgacgg ttgttgatta
    44641 ttattatctg actggcaata aaccgaatgc caccgtaatg gtcgatgttg atcgtcaggg
    44701 ctttgttgat ttactggctg atcgtctgaa attttacgct taatcactcg ccggatgaca
    44761 tcacgccatc cggcaccact catttcatta cggctcgaac ggacgtctct ggaactgatc
    44821 atgaccacat ttcgggcata gcgtcagcac ttccggtgta tagatcggga gatggaagtg
    44881 acatttctcg cagaccagat ttcccagccc taccacttct ccgctgtgat aaaccccgtg
    44941 atgattgagg tcctggaaaa cttcgcgcca ttcaagctgc gttttatcgg tgatgtctgc
    45001 cagctcctgc cacaagcttt ctttaatcac ccgcataaag acgctgtcag attcttcttt
    45061 caggctctct tcatagctca tggcgaactc ttccaggtca cgtctgacag ctcgcgtcag
    45121 ctcatcgacc tcggttcgcg ttaactcccc tgtttttatt acgcgctcgc gcgcctgttc
    45181 caccagtgcg tcgatatcac gttcgccatt gcgcaggcgt tcgctcagtg acgcgaccag
    45241 ttcacggtaa tattgagcaa ccttgttcat cgtttcatct cccgggtcgt taactgacta
    45301 taataataga cgttctttgt tccttgcttt gctaatacgc ccacaaactc tgtaaattaa
    45361 tgtatgaata atccgggcat catttcaacg tcgagtgcgc gaaaggctgt tttgacgcgg
    45421 acgtttgggc tatgctatgc ggatctgaaa aaccacctca acgctacatt tgtagccgta
    45481 ttgaaaacag gaccactggc tgccatgcaa gagcaatacc gcccggaaga gatagaatcc
    45541 aaagtacagc ttcattggga tgagaagcgc acatttgaag taaccgaaga cgagagcaaa
    45601 gagaagtatt actgcctgtc tatgcttccc tatccttctg gtcgactaca catgggccac
    45661 gtacgtaact acaccatcgg tgacgtgatc gcccgctacc agcgtatgct gggcaaaaac
    45721 gtcctgcagc cgatcggctg ggacgcgttt ggtctgcctg cggaaggcgc ggcggtgaaa
    45781 aacaacaccg caccggcacc gtggacgtac gacaacatcg cgtacatgaa aaaccagctc
    45841 aaaatgctgg gctttggtta tgactggagc cgcgagctgg caacctgtac gccggaatac
    45901 taccgttggg aacagaaatt cttcaccgag ctgtataaaa aaggcctggt atataagaag
    45961 acttctgcag tcaactggtg cccgaacgac cagaccgtac tggcgaacga acaagttatc
    46021 gacggctgct gctggcgctg cgataccaaa gttgagcgta aagagatccc gcaatggttt
    46081 atcaaaatca ccgcttacgc tgacgagctg ctcaacgatc tggataaact ggatcactgg
    46141 ccagacaccg ttaaaaccat gcagcgtaac tggatcggtc gttccgaagg cgtggaaatc
    46201 accttcaacg ttaacgacta tgacaacacg ctgaccgttt acactacccg cccggacacc
    46261 tttatgggtt gtacctacct ggcggtggct gcgggtcatc cgctggcaca gaaagcggcg
    46321 gaaaataatc ctgaactggc cgcctttatt gacgaatgcc gtaacaccaa agttgccgaa
    46381 gctgaaatgg cgacgatgga gaaaaaaggc gtcgatactg gctttaaagc ggttcaccca
    46441 ttaacgggcg aagaaattcc cgtttgggca gcaaacttcg tattgatgga atacggcacg
    46501 ggcgcagtta tggcggttcc gggtcacgac cagcgcgact acgagtttgc ctctaaatac
    46561 ggcctgaaca tcaaaccggt tatcctggca gctgacggct ctgagccaga tctttctcag
    46621 caagccctga ctgaaaaagg cgtgctgttc aactctggcg agttcaacgg tcttgaccat
    46681 gaagcggcct tcaacgccat cgccgataaa ctgactgcga tgggcgttgg cgagcgtaaa
    46741 gtgaactacc gcctgcgcga ctggggtgtt tctcgtcagc gttactgggg cgcgccgatt
    46801 ccgatggtga cgctggaaga cggtaccgta atgccgaccc cggacgacca gctgccggtg
    46861 atcctgccgg aagatgtggt gatggacggc attaccagcc cgattaaagc agatccggag
    46921 tgggcaaaaa ctaccgttaa cggtatgcca gcgctgcgtg aaaccgacac tttcgacacc
    46981 tttatggagt cctcctggta ctatgcgcgc tacacttgcc cggagtacaa agaaggtatg
    47041 ctggattcca aagcggctaa ctactggctg ccggtggata tctacattgg tggtatcgaa
    47101 cacgccatta tgcacctgct ctacttccgc ttcttccaca aactgatgcg tgatgcaggc
    47161 atggtgaact ctgacgaacc agcaaaacag ttgctgtgtc agggtatggt gctggcagat
    47221 gccttctact atgttggcga aaatggcgaa cgtaactggg tttccccggt tgatgctatc
    47281 gttgaacgtg acgagaaagg ccgtatcgtg aaagcgaaag atgcggcagg ccatgaactg
    47341 gtttataccg gcatgagcaa aatgtccaag tcgaagaaca acggtatcga cccgcaggtg
    47401 atggttgaac gttacggcgc ggacaccgtt cgtctgttta tgatgtttgc ttctccggct
    47461 gatatgactc tcgaatggca ggaatccggc gtggaagggg ctaaccgctt cctgaaacgt
    47521 gtctggaaac tggtttacga gcacacagcg aaaggtgatg ttgcggcact gaacgttgat
    47581 gcgctgactg aagatcagaa agcgctgcgt cgcgatgtgc ataaaacgat cgctaaagtg
    47641 accgatgata tcggccgtcg tcagaccttc aacaccgcaa ttgcggcgat tatggagctg
    47701 atgaacaaac tggcgaaagc accgaccgat ggcgagcagg accgcgccct gatgcaggaa
    47761 gcactgctgg ccgttgtccg tatgcttaac ccgttcaccc cgcacatctg cttcacgctg
    47821 tggcaggaac tgaaaggcga aggcgatatc gacaacgcgc cgtggccggt tgctgacgaa
    47881 aaagcgatgg tggaagactc cacgctggtc gtggtgcagg ttaacggtaa agtccgtgca
    47941 aaaatcaccg ttccggtgga cgcaacggaa gaacaggttc gcgaacgtgc tggccaggaa
    48001 catctggtag caaaatatct tgatggcgtt accgtgcgta aagtgattta cgtacccggt
    48061 aaactcctca atctggtcgt tggctaagcg cgggaggaag cgtgcgatat ctggcaacat
    48121 tgttgttatc tctggcggtg ttaatcaccg ccgggtgtgg ctggcatctg cgtgatacca
    48181 cgcaggttcc ttccactatg aaggtcatga tcctggactc aggcgatccg aacgggccat
    48241 taagccgtgc ggtgcgtaac cagttacgtc taaatggtgt cgagttgctt gataaagaaa
    48301 ccacgcgtaa ggacgttcca tccctgcgtt tgggtaaagt gagcatcgcg aaagataccg
    48361 catcggtatt ccgtaacggt caaacagcag agtatcagat gatcatgacg gtcaatgcga
    48421 ccgtgttgat ccccggccgt gacatctacc cgattagcgc caaagtcttc cgttcgttct
    48481 tcgataaccc gcaaatggcg ttagcgaaag ataacgaaca agacatgatc gtaaaagaga
    48541 tgtacgaccg tgctgccgaa cagctgattc gtaagctgcc aagcatccgt gctgcggata
    48601 ttcgttccga cgaagaacag acgtcgacca caacggatac tccggcaacg cctgcacgcg
    48661 tctctaccac gctgggtaac tgatgattcg gttgtacccg gaacaactcc gcgcgcagct
    48721 caatgaaggg ctgcgcgcgg cgtatctttt acttggtaac gatcctctgt tattgcagga
    48781 aagccaggac gctattcgtc aggtagctgc ggtacaagga ttcgaagaac accacacttt
    48841 ttccattgat cccaacactg actggaatgc gatcttttcg ttatgccagg ccatgagtct
    48901 gtttgccagt cgacaaacgc tattgctgtt gttaccagaa aacggaccga atgcggcgat
    48961 caatgaacaa cttctcacac tcaccggact tctgcatgac gacctgctgt tgatcgtccg
    49021 cggcaataaa ttaagcaaag cgcaagaaaa tgccgcctgg tttactgctc ttgcgaatcg
    49081 cagcgtgcag gtgacctgtc agacaccgga gcaggctcag cttccccgct gggttgctgt
    49141 gcgcgcaaaa cagctcaact tagagctgga tgacgcggcg aatcaggtgc tctgctactg
    49201 ttatgaaggt aacctgctgg cgctggctca ggcactggag cgtttatcgc tactctggcc
    49261 agacggcaaa ctgacattac ctcgcgttga acaggcggtg aatgatgccg cgcatttcac
    49321 cccttttcat tgggttgatg ctttgttgat gggaaaaagt aagcgcgcgt tgcatattct
    49381 tcagcaactg cgtctggaag gcagcgaacc ggttattttg ttgcgcacat tacaacgtga
    49441 actgttgtta ctggttaacc tgaaacgcca gtctgcccat atgccactgc gtgcgttgtt
    49501 tgataagcat cgggtatggc agaaccgccg gggcatgatg ggcgaggcgt taaatcgctt
    49561 aagccatccg cagttacgtc aggccgtgca actcctgaca cgaacggaac tcaccctcaa
    49621 acaagattac ggtcagtcag tgtgggcaga gcttgaaggg ttatctcttc tgttgtgcca
    49681 taaacccctg gcggacgtat ttatcgacgg ttgatatgaa atctttacag gctctgtttg
    49741 gcggcacctt tgatccggtg cactatggtc atcttaaacc cgtggaaacg ctggcgaatt
    49801 tgattggtct gacgcgggtc acaatcatcc ctaataatgt tcctccgcat cgtccccagc
    49861 cggaagcaaa cagcatgcag cgtaaacaca tgcttgaact ggcgattgcc gacaagccat
    49921 tatttactct tgatgaacgc gagctaaagc gcaatgcccc ctcttacact gcgcaaacac
    49981 tgaaagagtg gcggcaggag caaggaccgg acgtgccgct ggcgtttatt attggtcagg
    50041 attcattgct gacctttccg acctggtacg aatacgaaac gatactcgac aatgcacatt
    50101 tgatcgtctg tcggcgtcct ggttacccac ttgaaatggc acaaccacaa tatcagcaat
    50161 ggctggagga tcatttgaca cataacccgg aagatcttca ccttcagcct gccggtaaaa
    50221 tttatctggc tgaaacgccg tggtttaaca tctcggcgac catcatccgc gaacgtttgc
    50281 aaaatggtga atcgtgtgag gatttattac cggaaccggt actgacttac attaaccaac
    50341 aaggcttgta tcgctgatac ctgtcgttac ggagggcatg atgcgactgt ggttaattcg
    50401 tcatggtgaa acgcaagcga atgtcgatgg tctgtacagc ggtcatgcgc ccacacccct
    50461 gaccgcgcgc ggtattgagc aagcgcaaaa tctgcatacg ctgctacatg atgtttcctt
    50521 tgacctggtt ttatgcagtg aactggaacg ggcacagcat accgcgcgac tggttcttag
    50581 tgaccgccag caccccgttc atatcatccc tgaactcaat gaaatgtttt ttggcgactg
    50641 ggagatgcga catcatcgtg acctcatgca agaagatgcc gaaaactata gcgcgtggtg
    50701 caatgactgg cagcatgcta tccccacgaa cggtgaagga tttcaggcat tttcgcaacg
    50761 tgtggaacga tttatcgcaa ggcttagtga atatcagcac tatcagaata ttttagtcgt
    50821 cagccatcag ggcgtactga gtctgttaat tgctcgttta attggcatgc ctgccgagtc
    50881 tatgtggcat ttccgcgttg accaggggtg ttggagcgcc attgatatca accaaaaatt
    50941 cgcgacgcta cgcgtcctca atagccgtgc catcggggtc gagaatgcat gacttttctg
    51001 tttttttacg ggtaagccgc aacgaccatt gacagccccg ggcaggctga tattctccgc
    51061 agccagactt tttccgccag acacgacttt gtagaaattg ttttacaaaa atggcgatgc
    51121 aatctgcggc gcggggtggg atgatagccc actttcgaaa gccgattcgg cgacaattgt
    51181 cccgaaatcg cctctggttc aggtatactg acagaccatt tttatctatt tgattcaccc
    51241 agggggaaaa cttgcagggt aaagcactcc aggattttgt tatcgacaaa attgatgacc
    51301 tcaaaggtca ggacatcatc gccttagacg ttcagggcaa atccagcatc accgactgca
    51361 tgatcatctg tacgggtacg tccagccgcc atgttatgtc cattgctgat cacgttgtgc
    51421 aggagtctcg cgcagcgggc ctgttaccgc tcggcgtaga aggtgaaaac agcgccgact
    51481 ggattgtcgt ggatttgggc gatgtgattg tccatgtcat gcaggaagag agccgtcgcc
    51541 tgtatgaact ggaaaaactc tggagttaat gcgtgaagct gcaacttgtc gccgtgggaa
    51601 cgaaaatgcc ggactgggta caaaccggtt ttaccgagta cctgcgtcgt tttccgaaag
    51661 atatgccctt cgagctgatt gaaattccgg ccggaaaacg cggcaagaat gcggacatca
    51721 agcgcatact cgacaaagag ggtgagcaga tgttggcggc cgcaggcaaa aaccgcattg
    51781 tcaccctcga tattccaggc aagccctggg atacgccgca gttagccgct gagctggaac
    51841 gctggaagct ggatggtcgc gacgtcagtc tactgattgg cgggcctgaa gggttgtcgc
    51901 ctgcctgtaa agcggcggcg gagcagagct ggtcgctgtc ggcacttacc cttccccatc
    51961 cgctggttcg cgtgctggtc gcagagagtc tgtaccgggc gtggagcatc accaccaacc
    52021 atccttatca ccgtgagtga taagtgagct ttgagtagaa aacgcagcgg atgaaactac
    52081 agaactcttt tcgcgactat acggcagagt ccgcgctgtt tgtgcgccgg gcgctggtcg
    52141 cctttttggg gattttgctg ctgaccggcg tgcttatcgc caacctgtat aatctgcaaa
    52201 ttgttcgctt taccgactac cagacccgct ctaatgaaaa ccgcattaag ctggtgccta
    52261 tcgcacccag ccgcggcatt atctacgacc gtaacggtat ccctctggct ctcaaccgca
    52321 ctatctacca gatagaaatg atgccggaga aagtcgataa cgtgcagcaa acgctggacg
    52381 ctttgcgcag cgtggttgat ctgaccgatg atgatattgc ggcattccga aaagagcgcg
    52441 cacgttcaca ccgtttcacc tctattccgg tgaaaactaa cctgaccgaa gtacaagtag
    52501 ctcgctttgc cgtcaaccag taccgttttc cgggtgttga agttaaaggc tataaacgtc
    52561 gttactatcc ttacggttcg gcgttgaccc acgtcatcgg ctatgtgtcg aaaatcaacg
    52621 ataaagacgt cgaacgcctg aataatgacg gcaaactggc caactatgcg gcaacgcatg
    52681 atatcggtaa gctgggcatt gaacgttact atgaagacgt gctgcacggt cagaccggtt
    52741 atgaagaggt tgaagttaac aaccgtgggc gtgttatccg ccagttaaaa gaagtaccac
    52801 cgcaagccgg acacgatatt tacctgacgc tggatctcaa actccagcaa tatattgaaa
    52861 cgctgctggc gggtagccgc gcagctgtgg tagtcaccga tccgcgtaca ggtggggtgt
    52921 tggcgctggt ttccacgcct agttatgacc caaacctgtt tgttgacggt atctccagca
    52981 aagattattc cgccttgctg aacgacccga atacaccgct ggtgaaccgc gccacacagg
    53041 gggtttatcc tcccgcgtct acagttaaac cctatgttgc ggtttcggca ttgagtgccg
    53101 gggtgatcac gcgcaatacg acgctgtttg accccggctg gtggcaactg ccagggtcgg
    53161 aaaaacgtta tcgtgactgg aaaaaatggg gccacgggcg tctgaatgtc acaagatcgc
    53221 tggaagaatc tgcggatacc ttcttctatc aggtggccta cgatatgggg atcgatcgcc
    53281 tctccgaatg gatgggtaaa ttcggttatg gtcattacac cggtatcgac ctggcggaag
    53341 aacgttccgg caacatgcct acccgcgaat ggaaacagaa acgctttaaa aaaccgtggt
    53401 atcagggtga caccattccg gttggtatcg gtcagggtta ctggacagcg accccaatcc
    53461 agatgagtaa ggcactgatg atcctgatta atgacggtat cgtgaaggtt ccgcatttgc
    53521 tgatgagcac cgccgaagac ggcaaacagg tgccatgggt acaaccgcat gaaccgcccg
    53581 tcggcgatat tcattccggt tactgggagc tggcgaaaga cggtatgtac ggtgttgcta
    53641 accgccctaa cggtacggcg cataaatatt ttgccagcgc accgtacaaa attgcggcga
    53701 aatccggtac cgctcaggtc ttcggtctga aagcgaacga aacctataat gcgcacaaaa
    53761 ttgccgagcg tttacgtgac cacaaactga tgaccgcctt tgcgccatac aacaatccgc
    53821 aagtggctgt cgccatgatt ctggagaacg gtggcgcggg tccggcggtt ggtacgctga
    53881 tgcgccagat cctcgaccac attatgctgg gtgataacaa caccgatctg cctgcggaaa
    53941 atccagcggt tgccgcagcg gaggaccatt aatcatgaca gataatccga ataaaaaaac
    54001 attctgggat aaagtccatc tcgatcccac aatgctgctg atcttactgg cattgctggt
    54061 ttacagcgcc ctggttatct ggagcgccag cggtcaggat attggcatga tggagcgtaa
    54121 aatcggccag attgcgatgg gtctggtcat catggtggtg atggcgcaaa ttcctccacg
    54181 cgtttatgaa ggctgggccc cctatctcta tatcatctgt attattttgc tggtggcggt
    54241 agatgctttc ggtgccatct ctaaaggcgc tcaacgctgg ctggacctcg gtattgttcg
    54301 ttttcaaccg tcggaaattg ccaagatagc cgtaccgttg atggttgcac gctttatcaa
    54361 ccgcgacgtt tgcccacctt cattgaagaa tacagctatc gcgctggtgc tgatatttat
    54421 gcccacgctg ctggtggctg cacagcctga cttgggaaca tcaatcctcg ttgcgctttc
    54481 cggcctgttt gtactgttcc tctctggcct tagctggcgt ctgattggcg tcgcagtagt
    54541 gctggtagcg gcgttcattc cgattctgtg gttctttctg atgcatgatt accagcgcca
    54601 gcgcgtaatg atgctcctgg acccggaatc agacccactc ggcgcaggct atcacattat
    54661 tcagtctaaa attgctattg gctccggcgg attacgcggc aaaggctggc tgcacggcac
    54721 tcagtcacag cttgaatttc tccccgaacg ccataccgac tttatcttcg cggtactggc
    54781 ggaagagctg ggattagtgg gcattttgat tctgctcgct ctctacattc tgctgatcat
    54841 gcgcgggctg tggatagccg ccagagcgca aaccaccttt ggtcgcgtca tggctggcgg
    54901 cttaatgctg atattattcg tttatgtctt cgtaaatatt ggtatggtaa gcggtattct
    54961 gccggttgta ggggttccgc tcccactggt cagttatgga ggatcggcgc taattgtgct
    55021 gatggctggg ttcgggattg taatgtcaat ccacacccac aggaaaatgt tgtcgaaaag
    55081 cgtgtaagag gtgcgcaatg cgtaagcagt ggctcgggat ctgcatcgcg gcaggaatgc
    55141 tcgcggcatg tacaagcgat gatggtcagc aacagacggt aagtgtaccg cagcctgcgg
    55201 tatgtaacgg ccccatagtt gaaattagcg gggcagaccc gcgtttcgaa ccactgaacg
    55261 cgacggcaaa tcaggattac cagcgcgacg ggaaaagcta caaaatcgtg caggatccgt
    55321 ctcgatttag ccaggcggga ctggcggcaa tctatgatgc cgaacccggc agtaacctga
    55381 cggcctctgg cgaagctttc gatccgacac agttgacggc ggcccatcca acgcttccaa
    55441 tccccagcta cgccagaatc actaacctgg ctaacgggcg aatgatcgtg gtgcgcatta
    55501 atgatcgcgg cctttatggc aacgaccgcg tgatttcgct ttctcgcgcg gcagctgacc
    55561 gtcttaacac gtcaaacaac accaaagttc gtatcgatcc gattattgtc gcccaggatg
    55621 gttcgctttc tggtcctggt atggcttgta ccacagtcgc caaacagact tacgccctgc
    55681 cagcacctcc cgatttaagc ggtggcgcgg gaacaagttc agtgcctgtc ccgcagggtg
    55741 acattcttcc ggtcagtaat tcgacgctaa aaagcgaaga tccgaccggc gcgccggtaa
    55801 ccagcagcgg tttcctcggc gcaccaacga ccttagcgcc tggtgtactg gaaggcagcg
    55861 aaccgacgcc tgcgccacag cccgttgtta cagctccgtc gacaacgcct gcaacctcac
    55921 ctgcaatggt gacaccgcaa gccgcctcgc aaagcgccag cggcaacttt atggtgcaag
    55981 tcggggccgt tagcgatcag gctcgtgcgc aacagtacca acagcaactg ggacagaagt
    56041 tcggcgtccc cggtcgcgta actcaaaatg gcgcggtctg gcggatccag cttggcccat
    56101 tcgccagcaa agccgaagcc agtaccttgc agcaacgttt gcaaaccgaa gcccaattac
    56161 agtcatttat taccaccgcg cagtagcgta aagcaggcat ctgatgtgtc aatttctgta
    56221 aatgacgtta acaaagtcac gcggaaagtc agatgcctgc cggtagtgca tttgctatag
    56281 tagggcactt tttttaattc catcacggat gtcgtagttc agaccatgaa taccattttt
    56341 tccgctcgta tcatgaagcg cctggcgctc accacggctc tttgcacagc ctttatctct
    56401 gctgcacatg ccgatgacct gaatatcaaa accatgatcc cgggtgtacc gcagatcgac
    56461 gcggagtcct acatcctgat tgactataac tccggcaaag tactcgccga acagaacgct
    56521 gatgtccgcc gcgatcctgc cagcctgacc aaaatgatga ccagttacgt tatcggccag
    56581 gcaatgaaag ccggtaaatt taaagaaact gatttagtca ctatcggcaa cgacgcatgg
    56641 gccaccggta acccggtgtt taaaggttct tcgctgatgt tcctcaaacc gggcatgcag
    56701 gttccggttt ctcagctgat ccgcggtatt aacctgcaat cgggtaacga tgcttgtgtc
    56761 gccatggccg attttgccgc tggtagccag gacgcttttg ttggcttgat gaacagctac
    56821 gttaacgccc tgggcctgaa aaatacccac ttccagacgg tacacggtct ggatgctgat
    56881 ggtcagtaca gctccgcgcg agatatggcg ctgatcggcc aggcgttgat ccgtgacgta
    56941 ccgaatgaat actcgatcta taaagaaaaa gaatttacgt ttaacggtat tcgccagctg
    57001 aaccgtaacg gcctgttatg ggataacagc ctgaatgtcg acggcatcaa aaccggacac
    57061 actgacaaag caggttacaa ccttgttgct tctgcgactg aaggccagat gcgcttgatc
    57121 tctgcggtga tgggcggacg tacttttaaa ggccgtgaag ccgaaagtaa aaaattgcta
    57181 acctggggct tccgtttctt cgaaaccgtt aatcctctga aagtaggtaa agagttcgcc
    57241 tctgaaccgg tttggtttgg tgattctgat cgcgcttcgt taggggttga taaagacgtg
    57301 tacctgacca ttccgcgtgg tcgcatgaaa gatctgaaag ccagctatgt gctgaacagc
    57361 agtgaattgc atgcgccgct gcaaaagaat caggtcgtcg gaactatcaa cttccagctt
    57421 gatggcaaaa cgatcgaaca acgcccgctg gttgtgctgc aagaaatccc ggaaggtaac
    57481 ttcttcggca aaatcattga ttacattaaa ttaatgttcc atcactggtt tggttaaaaa
    57541 ttgaacactt gaaagtgtaa tttccgtccc catatactaa gcatcagtaa aaaactcccg
    57601 ccttctggcg ggagttgcta tttaattacg ttacgccgga gctgacatga aaaccaaact
    57661 taacgaactg cttgaattcc ctactccttt tacttacaaa gttatggggc aggcgttacc
    57721 tgagctggtt gatcaggtgg ttgaagtggt acagcgccat gcgccaggtg actacacccc
    57781 aacggtaaaa ccaagcagca aaggcaacta ccactcggta tctatcacta tcaacgccac
    57841 tcatatcgag caggttgaaa cactgtatga agaactgggc aaaatcgata ttgtccgcat
    57901 ggttctgtaa ctcgcttctc cgttacccgg ctcctggtcg ggtaactccc ctctcctcgc
    57961 tgtgatatac ttttccccca cttttactca ttctccacgg agatgccgtt ttgtatcagg
    58021 ataaaattct tgtccgccag ctcggtcttc agccttacga gccaatctcc caggctatgc
    58081 atgaattcac cgatacccgc gatgatagta cccttgatga aatctggctg gtcgagcact
    58141 atccggtatt cacccaaggg caggcaggaa aggcggagca cattttaatg ccgggtgata
    58201 ttccggtgat ccagagcgat cgcggtgggc aggtgactta tcacgggccg gggcaacagg
    58261 tgatgtatgt gttgcttaac ctgaaacgcc gtaaactcgg tgtgcgtgaa ctggtgacct
    58321 tgcttgagca aacagtggtg aataccctgg ctgaactggg tatagaagcg catcctcggg
    58381 ctgacgcgcc tggtgtctat gtcggggaaa agaaaatttg ctcactgggt ttacgtattc
    58441 gacgcggttg ttcattccac ggtctggcat taaacgtcaa tatggatctt tcaccatttt
    58501 tacgtattaa tccttgtggg tatgcaggaa tggaaatggc taaaatatca caatggaaac
    58561 ccgaagcgac gactaataat attgcaccac gtttactgga aaatatttta gcgctactaa
    58621 acaatcctga cttcgaatat attaccgctt aattccatac atcaatggcc caatttacac
    58681 tgggtcatta ctcataatac ctgctaatgc tttacatctc cttcgcgttt gtatattttc
    58741 tttatcactt gcagaaatgc gatgctacgc cttgctccgt agcccaccta attgattaac
    58801 ccattgaaat aattgaccat ggtgagcaaa agcgttccgt ctcaattaga cataagatta
    58861 ttgtaatttt tttaaatatt attttttcat gaataaaatt ggagtaagtg cgtggatagt
    58921 aataatcaaa ttgaaccctg tttaagcaga aaatcgtcag aaggtaaacc acaaatattt
    58981 acaaccttac gaaatattga tcttaccctt ctgactattt tgaagttgca tatgtccata
    59041 aaggaatcgt taatgcagcg aaagtgctta atctgccccc ctcggcaatc tatcagccta
    59101 ttcagaaact gcgcgttata ttccctgacc tattgtttat tcgcaaaggc cagggtgtca
    59161 ctcctaccgc atttgcgatg catctatatg agtctatcac tcagggactt gagtccattc
    59221 ttggcgcgct ggatatcgaa ggaagctatg ataagcaacg gacgataact attgcgccct
    59281 ccccctcggt cggggcccag gtccttcctg tcatctatcg ggcgattaaa actcactatc
    59341 cacagctttt actgcgcaac ccgcccatcg gcggcgcgga aaaccaactc tgtcagtttc
    59401 aaaccgatct catcaccgat aacatgtttt gcacaggacc atagatgtta tccgtaatgc
    59461 attttgaaaa agtattgtta cagccagatc acagcgcaga acgataaaat ggcacaatcc
    59521 aacaacaatt ttacattttc gcgaccgctt tggctgcttt caggtccgtt tcaatgatat
    59581 actgccggtc gttaattcaa aaatagttga taattacaac aatctattga attgaaacgc
    59641 tttccttcgt aattcgcaac tggaacacgc acgctatgag taaacccatt gtgatggaac
    59701 gcggtgttaa ataccgcgat gccgataaga tggcccttat cccggttaaa aacgtggcaa
    59761 cagagcgcga agccctgctg cgcaagccgg aatggatgaa aatcaagctt ccagcggact
    59821 ctacacgtat ccagggcatc aaagccgcaa tgcgcaaaaa tggcctgcat tctgtctgcg
    59881 aggaagcctc ctgccctaac ctggcggaat gcttcaacca cggcacagca acgtttatga
    59941 tcctcggcgc tatttgtacc cgccgttgtc cgttctgtga cgttgcccac ggtcgcccgg
    60001 tagctccgga tgccaatgaa ccagtgaaac tggcgcaaac cattgccgat atggcgctgc
    60061 gttatgtggt tatcacctcc gttgaccgtg atgacctgcg cgatggcggt gcccagcact
    60121 ttgcggattg cattactgcc attcgggaaa aaagcccgca aatcaaaatt gaaactctgg
    60181 tgccggattt ccgcggtcgt atggatcgtg ctctggatat tctgaccgcc acaccaccag
    60241 atgtgttcaa ccataacctg gaaaacgtac cgcgtattta ccgtcaggta cggcctggtg
    60301 cagattacaa ctggtcgctg aagctgctgg aacgctttaa agaagcgcat ccggaaatcc
    60361 cgaccaagtc tggtctgatg gtgggtctgg gtgaaaccaa tgaagaaatt attgaggtaa
    60421 tgcgcgacct gcgccgtcat ggtgtgacga tgctaacgct ggggcaatat ttgcagccaa
    60481 gccgccatca cctgccggtt caacgttacg ttagcccgga tgagttcgac gaaatgaaag
    60541 ccgaagcgct ggcgatgggc tttacccatg ctgcatgcgg tccgtttgtc cgctcgtctt
    60601 accacgccga tttgcaggcg aaagggatgg aagttaagta atcctgtaat aattacttac
    60661 atctgtctga taaaaaaccc gctttttgaa gcgggttttt tgtatcgaac aagatattga
    60721 gggagcgtcc tgctcgccac gtcactcttt atgagagagc ttttcagcct gaagatcaac
    60781 gtctgcgcct tttttcgccg cagcatcgtc atcattcatc gccttcttga accctttaat
    60841 ggccgctcca aggtctccgc ccagcgtacg taacttctta gtcccaaaca gcagaacgac
    60901 cagcgccgca actaccagca gtttggtaat actaatctca cccatagata ccttcttgac
    60961 atataaccgg caacagaacg cgccttaaaa accagagaat attaacggtc attcgacgcg
    61021 cgcacacaat agcaatctgt aacaaggtga atcaagcgcc gagtaaaaaa acatcataat
    61081 aattgcggca gcgcaaagcg acggttgttt aagacgggca gttgcgcgcg tacctgacgc
    61141 acacgttcgg gcgtcacttc cgccataatg agtgcaggca tttctgacgc tgccgcaatg
    61201 gtgacgccaa agggatctat aatccggctt tgcccgatat ttttgttccc gcactccccc
    61261 gccgccacca tataacaggt ggtatccagc acacgagcgg caagcaacgt tgaccagtga
    61321 tgctctttga gcggcccacg aacccaggcg gcaggaagta ccaggatttc agctccctgt
    61381 aatgcctgtg ccagcgccag ctctggaaag cgtaagtcat aacaggtcat cagaccgacc
    61441 ttcatccctt ccacctccag taacggagct atttcattac cagcatcaac atggcgtgat
    61501 tcctgaatgg caaatgcatc atagagatgc agtttggcat aacgggcgac gatgtttcct
    61561 gcctgtagcg ccaccagcat attccacgcg cgccccggtg ttgaaggaac atgaatcgtc
    61621 agaattgtcg tcatcatgtt acgtttactt tctcgccgta aacggtcgag gaattcgcct
    61681 tccagcagct gtgctgattt aaccgaaaga tccgcatcat gatcatcacg cgccagtaat
    61741 gcttccggca ggacaaacag cgaaacctcg ttttccgccg cctgcgccat caacgaggca
    61801 caaatctcag cgttcttttc ccacacagat gtaacagcaa actgtcctgc ggcaaccagc
    61861 attgccacct cctgtcagag tcgtaaaatt cgccaacggc gattattcgg cgttacactt
    61921 atcactcata caaatcaaat agcaggattt tgcagtgtta caacttcttt tagcagtttt
    61981 tattggcggt ggtacgggaa gcgtggcgag atggctgtta agtatgcgat ttaacccgct
    62041 gcatcaggcg attccgttgg ggacgctggc tgcaaacctg attggggcat tcatcatagg
    62101 aatgggattc gcctggttca gcaggatgac gaacattgat ccagtgtgga aagtattaat
    62161 caccaccgga ttttgtggcg gtctaacaac cttctcaaca ttttcggcag aagtggtgtt
    62221 tttgttacaa gagggccgct ttggctgggc gttactgaac gttttcgtca accttttagg
    62281 gtcttttgcc atgaccgcac tggctttctg gctgttttcc gcctcaaccg cacactaaag
    62341 accaaaaaaa acccgctgat taagcgggtt ttgaattctt gctgacgtat cttacagagc
    62401 gattacgttt gcagcagaag ggcctttggc accgttagtg atttcgaact ctacgcgctg
    62461 accttcagca agagttttaa aaccattagt ctggattgca gagaagtgta cgaacacgtc
    62521 tttgctgccg tcttccggag taatgaaacc gaatcctttg gactcattaa accacttaac
    62581 gttaccttta atcttagaca tcaaaattac ctttacatga aaaatagaca caaatgctgt
    62641 gtcggttacc agtacaccaa ttgtggtacg ctttgtccag tgcaacttta ctaaaaagtg
    62701 ataaaagtcg cgttattttt gatgttaatt attttaccca ttgattttaa agagtttact
    62761 aaaatttcat ttgtctcaaa actgaaagcg catccaggca aagtacacat tgccattgtt
    62821 ataggtaccc ggaatgtagg tcatctgaaa agtcactgga tcataaccca cagaggccaa
    62881 tggcagtaga accgggagag ggatgtaatt ccagttatcg cgtgccgtta cgccagcggt
    62941 gaatcccagt cctaaatgaa aattttcatc cgccagcggt cgccaggtac tttcccatcc
    63001 gtatccggca atcggttccc atttgttcca cgagtcctta aatgccatgg catacaggcc
    63061 atgccagttt cctttttcat cccaacgcga ctgaccaaaa ccgccacccc acggtcgctc
    63121 gttatagcga tcggtttttt ctttgtcgta agcgaaacgt gcatgccagg tgatggcagg
    63181 aatatataaa tcataatgtt caggctgttg ccaggtttgt gcaatatttt ctctaaacgt
    63241 tgtcatccac tcatctgcgt tagcaaaaac tttaccaacg ctgattaact gaataaaaac
    63301 aaaggaaaag atagcgacat atttactcac gttcatttgt gaccataaaa catttatcaa
    63361 aaatctacta ctagcatagc aaagctacaa ttaacataac cttaatagcc ccaacataaa
    63421 gaataatctg aatagctgct ctatttattt ccgcttgttt aagttaaata tttccgcgca
    63481 tcgattaaag atgatgcgtc gttttctgcg atgggaatag tcaaaaaaga aaaatcaagt
    63541 cttttttgat gacaaaatgc aatcaaggaa aaattatttt attttttaac gagatacaac
    63601 aatca
//
LOCUS       DAZGQL010000014        63534 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.014, whole genome shotgun sequence.
ACCESSION   DAZGQL010000014 DAZGQL010000000
VERSION     DAZGQL010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 63534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63534
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.014"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..415
                     /locus_tag="ACHKIR_001077"
     CDS             <1..415
                     /locus_tag="ACHKIR_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708149.1"
                     /note="catalyzes the formation of (1 R,6
                     R)-2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     in menaquinone biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HFO9988845.1"
                     /translation="QNAEQRTERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDE
                     QRRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALA
                     AELAAECHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            430..1287
                     /gene="menB"
                     /locus_tag="ACHKIR_001078"
     CDS             430..1287
                     /gene="menB"
                     /locus_tag="ACHKIR_001078"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HFO9988846.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            1287..2249
                     /gene="menC"
                     /locus_tag="ACHKIR_001079"
     CDS             1287..2249
                     /gene="menC"
                     /locus_tag="ACHKIR_001079"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HFO9988847.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQSGLLAWVNNWLAGDSELPQMPSVAFGVSCALAELADTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRDRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSLLPLVDVDALERLL"
     gene            2246..3601
                     /gene="menE"
                     /locus_tag="ACHKIR_001080"
     CDS             2246..3601
                     /gene="menE"
                     /locus_tag="ACHKIR_001080"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HFO9988848.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELASSF
                     AMQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQRVEGAHAATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAEGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNDEVWLRAASMVEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVVEYDQQTVDLDEWVKDKLARFQQPVRWLNLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            3711..3977
                     /gene="pmrD"
                     /locus_tag="ACHKIR_001081"
     CDS             3711..3977
                     /gene="pmrD"
                     /locus_tag="ACHKIR_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HFO9988849.1"
                     /translation="MEWLVKKSCCNKQDNRHVIMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASCYSPDEWERQCKAAGKTQ"
     gene            complement(3971..4357)
                     /gene="arnF"
                     /locus_tag="ACHKIR_001082"
     CDS             complement(3971..4357)
                     /gene="arnF"
                     /locus_tag="ACHKIR_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HFO9988850.1"
                     /translation="MGLMWGLFSVIIASAAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(4357..4692)
                     /gene="arnE"
                     /locus_tag="ACHKIR_001083"
     CDS             complement(4357..4692)
                     /gene="arnE"
                     /locus_tag="ACHKIR_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HFO9988851.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            complement(4689..6341)
                     /gene="arnT"
                     /locus_tag="ACHKIR_001084"
     CDS             complement(4689..6341)
                     /gene="arnT"
                     /locus_tag="ACHKIR_001084"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HFO9988852.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLAAVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFAMDDAQHRAPFWYYLPVIIAGSLPWLGLLP
                     GALYAGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVVSPWGAMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRNEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            complement(6341..7231)
                     /gene="arnD"
                     /locus_tag="ACHKIR_001085"
     CDS             complement(6341..7231)
                     /gene="arnD"
                     /locus_tag="ACHKIR_001085"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HFO9988853.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQAHSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQQVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSGMLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            complement(7228..9210)
                     /gene="arnA"
                     /locus_tag="ACHKIR_001086"
     CDS             complement(7228..9210)
                     /gene="arnA"
                     /locus_tag="ACHKIR_001086"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HFO9988854.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VARLAAERGIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRIAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPTIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQTLGLVQGSRLNSQPACAARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LTVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NARRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            complement(9210..10178)
                     /gene="arnC"
                     /locus_tag="ACHKIR_001087"
     CDS             complement(9210..10178)
                     /gene="arnC"
                     /locus_tag="ACHKIR_001087"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HFO9988855.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTAACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            complement(10182..11321)
                     /gene="arnB"
                     /locus_tag="ACHKIR_001088"
     CDS             complement(10182..11321)
                     /gene="arnB"
                     /locus_tag="ACHKIR_001088"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HFO9988856.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKSTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     HTWGRAPQAEVLTPGYKYNLTDINAAIALTQLVKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWSHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            11629..12231
                     /gene="ais"
                     /locus_tag="ACHKIR_001089"
     CDS             11629..12231
                     /gene="ais"
                     /locus_tag="ACHKIR_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II)-phosphate
                     phosphatase Ais"
                     /protein_id="HFO9988857.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQRKAPDKNIVIFTHNH
                     CLTYIAKDKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            complement(12270..12695)
                     /gene="nudI"
                     /locus_tag="ACHKIR_001090"
     CDS             complement(12270..12695)
                     /gene="nudI"
                     /locus_tag="ACHKIR_001090"
                     /EC_number="3.6.1.12"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HFO9988858.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVEPGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLAHYDLNVATRKTLRLKGLL"
     gene            12974..13516
                     /gene="yfaZ"
                     /locus_tag="ACHKIR_001091"
     CDS             12974..13516
                     /gene="yfaZ"
                     /locus_tag="ACHKIR_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaZ family outer membrane protein"
                     /protein_id="HFO9988859.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            13616..14818
                     /gene="yfaY"
                     /locus_tag="ACHKIR_001092"
     CDS             13616..14818
                     /gene="yfaY"
                     /locus_tag="ACHKIR_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HFO9988860.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLTRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEKQAMEKLWLDVKRVAGQSVIFEGTEGLPSQISRELQTRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            15038..15820
                     /gene="yfaX"
                     /locus_tag="ACHKIR_001093"
     CDS             15038..15820
                     /gene="yfaX"
                     /locus_tag="ACHKIR_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFO9988861.1"
                     /translation="MLESSKVPALTRAIDILNLIARIGPCSAATIIDTLGIPKSTAYL
                     LLNELRRQRFLSLDHQENFCLWTRLVELSGHALSKMDLRELARPRLTQLMDTTGLLCH
                     LGIIDNGSAYYILKVESSATISVRSHEGKSLSLYRSGIGKCLLAWQPAAVQQSIIEGL
                     IWEQATPTTITHPQQLHEELARIRRQGWSYDNGEDYADVRCVAAPVFNANNELTAAIS
                     VVGTRLQINEEYRDYLAGKAIACARDISRLLGWKSPFDLQAS"
     gene            15835..17040
                     /gene="rhmD"
                     /locus_tag="ACHKIR_001094"
     CDS             15835..17040
                     /gene="rhmD"
                     /locus_tag="ACHKIR_001094"
                     /EC_number="4.2.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050032 - L-rhamnonate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
                     /protein_id="HFO9988862.1"
                     /translation="MTLPKIKQVRAWFTGGATAEKGAGGGDYHDQGANHWIDDHIATP
                     MSKYRDYEQSRQSFGINVLGTLIVEVEAENGQTGFAVSTAGEMGCFIVEKHLNRFIEG
                     KCVSDIKLIHDQMLNATLYYSGSGGLVMNTISCVDLALWDLFGKVVGLPVYKLLGGAV
                     RDEIQFYATGARPDLAKEMGFIGGKMPTHWGPHDGDAGIRKDAAMVADMREKCGEDFW
                     LMLDCWMSQDVNYATKLAHACAPYNLKWIEECLPPQQYEGYRELKRNAPVGMMVTSGE
                     HHGTLQSFRTLSETGIDIMQPDVGWCGGLTTLVEIAAIAKSRGQLVVPHGSSVYSHHA
                     VISFTNTPFSEFLMTSPDCSTMRPQFDPILLNEPVPVNGRIHKSVLDKPGFGVELNRD
                     CNLKRPYNH"
     gene            17097..18386
                     /gene="yfaV"
                     /locus_tag="ACHKIR_001095"
     CDS             17097..18386
                     /gene="yfaV"
                     /locus_tag="ACHKIR_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9988863.1"
                     /translation="MSTALLDAVVKKNRARLIPFMLALYVLAFLDRSNIGFAKQTYQI
                     DTGLSNEAYALGAGIFFVVYAFLGVPANLLMRKLGARTWIGTTTLLWGFLSAAMAWAD
                     TEAKFLIVRTLLGAAEAGFFPGMIYLTSQWFPQRNRASIMGLFYMGAPLALTLGSPLS
                     GALLEMHGFMGHPGWFWMFVIEGLLAVGAGVFTFFWLDDTPEQARFLSKEEKTLLINQ
                     LASEEQQKVTSRLSDALRNGRVWQLAIIYLTIQVAVYGLIFFLPTQVAALLGTKVGFT
                     ASVVTAIPWVAALFGTWLIPRYSDKTGERRNVAALTLLAAGIGIGLSGLLSPVLAIVA
                     LCVAAIGFIAVQPVFWTMPTQLLSGTALAAGIGFVNLFGAVGGFIAPILRVKAETLFA
                     SDAAGLLTLAAVAVIGSLIIFTLRVNRTVAQTDVAHH"
     gene            18403..19206
                     /gene="yfaU"
                     /locus_tag="ACHKIR_001096"
     CDS             18403..19206
                     /gene="yfaU"
                     /locus_tag="ACHKIR_001096"
                     /EC_number="4.1.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxy-L-rhamnonate aldolase"
                     /protein_id="HFO9988864.1"
                     /translation="MNALLTNPFKERLRKGEVQIGLWLSSTTAYMAEIAATSGYDWLL
                     IDGEHAPNTIQDLYHQLQAVAPYASQPVIRPVEGSKSLIKQVLDIGAQTLLIPMVDTA
                     DQARQVVSATRYPPYGERGVGASVARAARWGRIENYMAHVNDSLCLLVQVESKTALDN
                     LDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAADKAAGFLAV
                     APDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGNY"
     gene            complement(19247..19480)
                     /locus_tag="ACHKIR_001097"
                     /pseudo
     CDS             complement(19247..19480)
                     /locus_tag="ACHKIR_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(19481..20476)
                     /locus_tag="ACHKIR_001098"
     CDS             complement(19481..20476)
                     /locus_tag="ACHKIR_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HFO9988865.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDSEGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIEEIAERSPLQKERLMTIAERLRQEGHQIGWQEGKIEGWQEGKIEGWQ
                     EGKLEGLQEGKLDGLQEGMHEQAIKIALRMLEQGFEREIVLATTQLTDADIPNCH"
     gene            complement(20669..21859)
                     /gene="glpC"
                     /locus_tag="ACHKIR_001099"
     CDS             complement(20669..21859)
                     /gene="glpC"
                     /locus_tag="ACHKIR_001099"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit GlpC"
                     /protein_id="HFO9988866.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSVAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(21856..23115)
                     /gene="glpB"
                     /locus_tag="ACHKIR_001100"
     CDS             complement(21856..23115)
                     /gene="glpB"
                     /locus_tag="ACHKIR_001100"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HFO9988867.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVTDIHSGLEALRQQAPAHPYTLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRVTPLGTLRSTWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLAVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWSLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERDGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQLFGVTTDEALRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQL"
     gene            complement(23105..24733)
                     /gene="glpA"
                     /locus_tag="ACHKIR_001101"
     CDS             complement(23105..24733)
                     /gene="glpA"
                     /locus_tag="ACHKIR_001101"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046174 - polyol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HFO9988868.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQRIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTMSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            25006..26364
                     /gene="glpT"
                     /locus_tag="ACHKIR_001102"
     CDS             25006..26364
                     /gene="glpT"
                     /locus_tag="ACHKIR_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015169 - glycerol-3-phosphate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HFO9988869.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHQELLQKRN
                     GG"
     gene            26369..27445
                     /gene="glpQ"
                     /locus_tag="ACHKIR_001103"
     CDS             26369..27445
                     /gene="glpQ"
                     /locus_tag="ACHKIR_001103"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFO9988870.1"
                     /translation="MKLTLKNLSMAIMMSGMIMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYSGKDDKVYLQCFDADELKRI
                     KNELEPKMGMDLNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            27648..28298
                     /gene="inaA"
                     /locus_tag="ACHKIR_001104"
     CDS             27648..28298
                     /gene="inaA"
                     /locus_tag="ACHKIR_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HFO9988871.1"
                     /translation="MAVSAKHDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYAQHAVTPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKSEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            complement(28352..28606)
                     /gene="yfaE"
                     /locus_tag="ACHKIR_001105"
     CDS             complement(28352..28606)
                     /gene="yfaE"
                     /locus_tag="ACHKIR_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HFO9988872.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEL"
     gene            complement(28606..29736)
                     /gene="nrdB"
                     /locus_tag="ACHKIR_001106"
     CDS             complement(28606..29736)
                     /gene="nrdB"
                     /locus_tag="ACHKIR_001106"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFO9988873.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDSEVDTDDLSNFQL"
     gene            complement(29802..32087)
                     /gene="nrdA"
                     /locus_tag="ACHKIR_001107"
     CDS             complement(29802..32087)
                     /gene="nrdA"
                     /locus_tag="ACHKIR_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFO9988874.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            32215..32358
                     /gene="ypaB"
                     /locus_tag="ACHKIR_001108"
     CDS             32215..32358
                     /gene="ypaB"
                     /locus_tag="ACHKIR_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpaB"
                     /protein_id="HFO9988875.1"
                     /translation="MALLQVHNFVDNSGRKKWLSRTLGQTRCPGKSMGREKFVKNNCS
                     VIS"
     gene            32782..36540
                     /gene="yfaL"
                     /locus_tag="ACHKIR_001109"
     CDS             32782..36540
                     /gene="yfaL"
                     /locus_tag="ACHKIR_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HFO9988876.1"
                     /translation="MRIIFLRKEYLSLLPSMIASLFSGNGAAAVIDSCQGYDIKANCQ
                     ASRQSLSGNMQDWSIADGQWLIFSGMANNASGGAVFLQQSAEFTISPQNETGMTLFAD
                     NSVSGEYNNGGAIFAKENSTINLANVIFDSNVAGGYGGAIYSAGTNDTGAADLRVTNA
                     VFHNNIANDGKGGAIYTINNDVYLSDDAFNNNQAYTSTSYSDGDGGAIDVTDNSTDNT
                     HLSGKTIINNTSFTNNYAEGYGGAIYTSSTTSPYLIDISVDDNYDQNNGVMIDENNSA
                     SGYDHSATAAAGGFMYIGHSVAEFNIAADKTLVIGNTSNDGAIDSLAGTGVIVKEGAG
                     ELVLNADNNAFTGEISIQNGEVTLGRSDELMNVGDTHCQSDPQDCFGLMVGSTVHSEY
                     QAELNVGNTQQTFVHSLTGFANGILNIDAGGNVTVNQGGFSGSIQGEGQLTVAQDGSY
                     LLTGAQSMALTGDIVVEDNAVLSLAGNQADLRAMQSDPQSIVLNGGVLDLSDFTTWDG
                     DSSYNDGLQISGSGGTVIGSNDVVDISSGDDLHIGGSDASQNGVYVVINAGDQRVTLA
                     NNNGYLGNTQIASGTLEVSDNSQLGDTSYNRSVIFTDPQQHSEMDVTTDVDTRSATTG
                     QGRNIEMRADGEIHVEDGVDTQWGGLMADSTGQQLESVSTLTKSGGGTLELTASGTAT
                     SAVRVEDGTLKGEAENIIPYVSSLWVGEDGVFETGQNQDIRSIDATSGGDIDITDGTV
                     LRLTQQDTNQVLDASLFSGDGTLVNATDGVTLTGELNTNLETDSLTYLSDVTVNGNLT
                     NTSGAISLQNGVAGDTLTVNGDYTGGGTLLLDSELNGDDSASDQLVLNGNTAGNTAVV
                     INPITGIGEPTSTGIKVVDFAADPTQFQNNAQFRLAGSGYVNMGAYDYTLVEDNNDWY
                     LRSQEVTPTPPPDPDPTPDPDPTPDPDPTPDPGPTPAYQPVLNAKVGGYFNNLRAANQ
                     AFVMERRDQAGGDGQTLNLRVIGGRYHYTAAGQLAQHEDTSTVQLSGNLFSGHWGDDG
                     EWMLGIVGGYSDNQGDSRSNMTGTRADNQNHGYAVGLTSSWYQHGNQKQGVWLDSWLQ
                     YAWFNNDVSEQDDGEDHYHSSGIIASLEAGYQWLPGHGVVIEPQTQVIYQGVQQDDFT
                     AANHARVSQSQGDDIQTRLGLHSEWSTAVGVTPTLDLNYYHDPHATEIEEDGSTISDD
                     GVKQRGEIKVGVTGNISQRVSLRGSVAWQKGSDDFAQTAGFLSMTVKW"
     gene            complement(36680..37402)
                     /gene="ubiG"
                     /locus_tag="ACHKIR_001110"
     CDS             complement(36680..37402)
                     /gene="ubiG"
                     /locus_tag="ACHKIR_001110"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HFO9988877.1"
                     /translation="MNAEKSPVNHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVEYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHMTGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            37549..40176
                     /gene="gyrA"
                     /locus_tag="ACHKIR_001111"
     CDS             37549..40176
                     /gene="gyrA"
                     /locus_tag="ACHKIR_001111"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HFO9988878.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLIIQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVEGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            40325..42013
                     /gene="yfaA"
                     /locus_tag="ACHKIR_001112"
     CDS             40325..42013
                     /gene="yfaA"
                     /locus_tag="ACHKIR_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HFO9988879.1"
                     /translation="MSGEKKAKGWRFYGLVGLGAIVLLSAGVWALQYAGSGPEKTLSP
                     LVVHNNLQIDLNEPDLFLDSDSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIE
                     GSIRRIVYEHDLTLKDKLFSSLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPL
                     LFAATSDSQLSKTEISSIKLNSETIPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFK
                     DDQQDTEATAIASDLLSGKKRWQASFGLEERAAEKTPVRQRIVVSARLLGFGYQRLMP
                     SFAGVRFEMSNDGWHSFVALNDESASVDASFDFTPVWNSMPAGASFCVAVPYSHGIAE
                     EMLSHISQENDKLNGALDGAAGLCWYEDSKLQTPLFVGQFDGSAEQAQLPGKLFTQNI
                     GAHESKAPEGVLPVNQTQQGEAQIWRREVSSRYGQYPKAQAVQPDQLMSDYFFRVSLA
                     MQNKTMLFSLDDTLVNNALQTLNKTRPAMVDVIPTDGIVPLYINPQGVAKLLRNETLT
                     SLPKNLEPVFYNAAQTLLMPKLDALSQQPRYVMKLAQMEPGAAWQWLPITWQPL"
     gene            42010..42633
                     /gene="yfaT"
                     /locus_tag="ACHKIR_001113"
     CDS             42010..42633
                     /gene="yfaT"
                     /locus_tag="ACHKIR_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HFO9988880.1"
                     /translation="MRHGLLALICWLCCVVTHSEMLNVEQSGLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGLSNQYLPPEMTLTPEQRQLAQNW
                     NQGNGKTGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            42657..47171
                     /locus_tag="ACHKIR_001114"
     CDS             42657..47171
                     /locus_tag="ACHKIR_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0010951 - negative regulation of
                     endopeptidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
                     /protein_id="HFO9988881.1"
                     /translation="MGTGLANADDSLPSSNYAPPAGGTFFLLADSSFSSSEEAKVRLE
                     APGRDYRRYQMEEYGGVDVRLYRIPDPMAFLRQQKNLHRIVVQPQYLGDGLNNTLTWL
                     WDNWYGKSRRVMQRTFSSQSRQNVTQALPELQLGNAIIKPSRYVQNNQFSPLKKYPLV
                     EQFRYPLWQAKPVEPQQGVKLEGASSNFISPQPGNIYIPLGKQEPGLYLVEAMVGGYR
                     ATTVVFVSDTVALSKVSGNELLVWTAGKKQGEAKPGSEILWTDGLGVMTRGVTDDSGT
                     LQLQHISPERSYILGKDAEGGVFVSENFFYESEIYNTRLYIFTDRPLYRAGDRVDVKV
                     MGREFHDPLHSSPIVSAPAKLSVLDANGSLLQTVNVTLDARNGGQGSFRLPENAVAGG
                     YELRLAYRKQVYSSSFRVANYIKPHFEIGLALDKKEFKTGEAVSGKLQLLYPDGEPVK
                     DARVQLSLRAQQLSMVGNDLRYAGRFPVSLEGSETVSDDSGHVALNLPAADKPSRYLL
                     TVSASDGAAYRVTTTKEILIERGLAHYSLSTAAQYSNSGESVVFRYAALESSKQVPVT
                     YEWLRLEDRTSHSGDLPSGGKSFTVNFDKPGNYNLTLRDEDGLILAGLSHAVSGKGST
                     AHTGTVDIVADKTLYQPGETAKMLITFPEPIDEALLTLERDRVEQQSLLSHPANWLTL
                     QRLNDTQYEARIPVSNSFAPNITFSVLYTRNGQYSFQNAGIKVAVPQLDIRVKTEKTH
                     YQPGELVNVELTSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRNNVRT
                     SSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPSLTTDKQGKA
                     YFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVYRMGDKPAAG
                     LFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLTQNIQQSGLLSAELQQNGQV
                     QDSISTKLSFVDNSWPVEQQKNVMLGGGDNALMLPEQASNIQLQSSETPQEIFRNNLD
                     ALVDEPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGPGAR
                     FTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPDYWQHMLDSYAEQADNMPILHRA
                     LVLAWAQEMNLPCKTLLKGLDEAIARRGTKTEDFSEEDTSDINDSLILDTPESPLADA
                     VANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQAAAIL
                     SGLTAEQSTIERALAMNWLAKYMATMPPVVLPAPAGAWAKHKLTGGGEDWRWVGQGVP
                     DILSFGDELSPQNVQVRWREPAKTAQQSNIPVTVERQLYRLIPGEEEMSFTLQPVTSN
                     EIDSDALYLDEITLTSEQDAVLRYGQVEVPLPPGADVERTTWGISVNKPNAGKQQGQL
                     LEKARNEMGKLAYMVPVKELTGTVTFRHLLRFSQKGQFVLPPARYVRSYAPAQQSVAA
                     GSEWTGMQVK"
     gene            47172..48821
                     /gene="yfaQ"
                     /locus_tag="ACHKIR_001115"
     CDS             47172..48821
                     /gene="yfaQ"
                     /locus_tag="ACHKIR_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HFO9988882.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDTLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVNSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVLVELFARYPLKKITAEKSS
                     TAVKPGVLNGRYRVTFANGNHIMFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTFQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            48826..49602
                     /gene="yfaP"
                     /locus_tag="ACHKIR_001116"
     CDS             48826..49602
                     /gene="yfaP"
                     /locus_tag="ACHKIR_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HFO9988883.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPIHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(49676..50860)
                     /gene="atoB"
                     /locus_tag="ACHKIR_001117"
     CDS             complement(49676..50860)
                     /gene="atoB"
                     /locus_tag="ACHKIR_001117"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HFO9988884.1"
                     /translation="MKNCVIVSAVRTAIGSFNGSLASTSAIDLGATVIKAAIERAKID
                     SLHVDEVIMGNVLQAGLGQNPARQALLKSGLAETVCGFTVNKVCGSGLKSVALAAQAI
                     QAGQAQSIVAGGMENMSLAPYLLDAKARSGYRLGDGQVYDVILRDGLMCATHGYHMGI
                     TAENVAKEYGITREMQDELALHSQRKAAAAIESGAFTAEIVPVNVVTRKKTFVFSQDE
                     FPKADSTTEALGALRPAFDKAGTVTAGNASGINDGAAALVIMEESAALAAGLTPLARI
                     KSYASGGVPPALMGMGPVPATKKALQLAGLQLADIDLIEANEAFAAQFLAVGKTLGFD
                     SEKVNVNGGAIALGHPIGASGARILVTLLHAMQARDKTLGLATLCIGGGQGIAMVIER
                     LN"
     gene            complement(50891..52213)
                     /gene="atoE"
                     /locus_tag="ACHKIR_001118"
     CDS             complement(50891..52213)
                     /gene="atoE"
                     /locus_tag="ACHKIR_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HFO9988885.1"
                     /translation="MIGRISRFMTRFVSRWLPDPLIFAMLLTLLTFVIALWLTPQTPI
                     SMVKMWGDGFWNLLAFGMQMALIIVTGHALASSAPVKSLLRTAASAAKTPVQGVMLVT
                     FFGSVACVINWGFGLVVGAMFAREVARRVPGSDYPLLIACAYIGFLTWGGGFSGSMPL
                     LAATPGNPVEHIAGLIPVGDTLFSGFNIFITVALIVVMPFITRMMMPKPSDVVSIDPK
                     LLMEEADFQKQLPKDAPPSERLEESRILTLIIGALGIAYLAMYFSEHGFNITINTVNL
                     MFMIAGLLLHKTPMAYMRAISAAARSTAGILVQFPFYAGIQLMMEHSGLGGLITEFFI
                     NVANKDTFPIMTFFSSALINFAVPSGGGHWVIQGPFVMPAAQALGADLGKSVMAIAYG
                     EQWMNMAQPFWALPALAIAGLGVRDIMGYCITALLFSGVIFVIGLTLF"
     gene            complement(52210..52860)
                     /gene="atoA"
                     /locus_tag="ACHKIR_001119"
     CDS             complement(52210..52860)
                     /gene="atoA"
                     /locus_tag="ACHKIR_001119"
                     /EC_number="2.8.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit beta"
                     /protein_id="HFO9988886.1"
                     /translation="MDAKQRIARRVAQELRDGDIVNLGIGLPTMVANYLPEGIHITLQ
                     SENGFLGLGPVTTAHPDLVNAGGQPCGILPGAAMFDSAMSFALIRGGHIDACVLGGLQ
                     VDEEANLANWVVPGKMVPGMGGAMDLVTGSRKVIIAMEHCAKDGSAKILRRCTMPLTA
                     QHAVHMLVTELAVFRFIDGKMWLTEIADGCDLATVRAKTEARFEVAADLNTQRGDL"
     gene            complement(52860..53522)
                     /gene="atoD"
                     /locus_tag="ACHKIR_001120"
     CDS             complement(52860..53522)
                     /gene="atoD"
                     /locus_tag="ACHKIR_001120"
                     /EC_number="2.8.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit alpha"
                     /protein_id="HFO9988887.1"
                     /translation="MKTKLMTLQDATGFFRDGMTIMVGGFMGIGTPSRLVEALLESGV
                     RDLTLIANDTAFVDTGIGPLIVNGRVRKVIASHIGTNPETGRRMISGEMDVVLVPQGT
                     LIEQIRCGGAGLGGFLTPTGVGTVVEEGKQTLTLDGKTWLLERPLRADLALIRAHRCD
                     TLGNLTYQLSARNFNPLIALAADITLVEPDELVETGELQPDHIVTPGAVIDHIIVSQE
                     SK"
     gene            complement(53718..55103)
                     /gene="atoC"
                     /locus_tag="ACHKIR_001121"
     CDS             complement(53718..55103)
                     /gene="atoC"
                     /locus_tag="ACHKIR_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate metabolism transcriptional
                     regulator AtoC"
                     /protein_id="HFO9988888.1"
                     /translation="MTAINRILIVDDEDNVRRMLSTAFALQGFETHCANNGRTALHLF
                     ADIHPDVVLMDIRMPEMDGIKALKEMRSNETRTPVILMTAYAEVETAVEALRCGAFDY
                     VIKPFDLDELNLIVQRALQLQSMKKEIRHLHQALSTSWQWGHILTNSPAMMDICKDTA
                     KIALSQASVLISGESGTGKELIARAIHYNSRRAKGPFIKVNCAALPESLLESELFGHE
                     KGAFTGAQTLRQGLFERANEGTLLLDEIGEMPLVLQAKLLRILQEREFERIGGHQTIK
                     VDIRIIAATNRDLQAMVKEGTFREDLFYRLNVIHLILPPLRDRREDISLLANHFLQKF
                     SSENQRDIIDIDPMAMSLLTAWSWPGNIRELSNVIERAVVMNSGPIIFSEDLPPQIRQ
                     PVCNAGEAKTAPVGERNLKEEIKRVEKRIIMEVLEQQEGNRTRTALMLGISRRALMYK
                     LQEYGIDPADV"
     gene            complement(55100..56926)
                     /gene="atoS"
                     /locus_tag="ACHKIR_001122"
     CDS             complement(55100..56926)
                     /gene="atoS"
                     /locus_tag="ACHKIR_001122"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     AtoS"
                     /protein_id="HFO9988889.1"
                     /translation="MHYMKWIYPRRLRNQMILMAILMVIVPTLTIGYIVETEGRSAVL
                     SEKEKKLSAVVNLLNQALGNRYDLYIDLPREERIRALNAELAPITENITHAFPGIGAG
                     YYNKTLDAIITYAPSALYQNNVGVTIAADHPGREVMRTNTPLVYSGRQVRGDILNSMI
                     PIERNGEILGYIWANELTEDIRRQAWKMDVRIIIVLTAGLLISLLLIVLFSRRLSANI
                     DIITDGLSTLAQNIPTRLPQLPGEMGQISQSVNNLAQALRETRTLNDLIIENAADGVI
                     AIDRQGDVTTMNPAAEVITGYQRHELVGQPYSMLFDNTQFYSPVLDTLEHGTEHVALE
                     ISFPGRDRTIELSVTTSRIHNTHGEMIGALVIFSDLTARKETQRRMAQAERLATLGEL
                     MAGVAHEVRNPLTAIRGYVQILRQQTRDPIHQEYLSVVLKEIDSINKVIQQLLEFSRP
                     RHSQWQQVSLNALVEETLVLVQTAGVQARVDFISELDNELSPINADRELLKQVLLNIL
                     INAVQAISARGKIRIRTWQYSDSQQAISIEDNGCGIDLSLQKKIFDPFFTTKASGTGL
                     GLALSQRIINAHQGDIRVASLPGYGATFTLILPINPQGNQTV"
     gene            57093..59942
                     /gene="rcsC"
                     /locus_tag="ACHKIR_001123"
     CDS             57093..59942
                     /gene="rcsC"
                     /locus_tag="ACHKIR_001123"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0018106 - peptidyl-histidine
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HFO9988890.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQTDVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPLTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(60028..60678)
                     /gene="rcsB"
                     /locus_tag="ACHKIR_001124"
     CDS             complement(60028..60678)
                     /gene="rcsB"
                     /locus_tag="ACHKIR_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor RcsB"
                     /protein_id="HFO9988891.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(60723..62012)
                     /locus_tag="ACHKIR_001125"
     CDS             complement(60723..62012)
                     /locus_tag="ACHKIR_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO9988892.1"
                     /translation="MSNASYPTGVENHGGSLRIWFHYNGKRVRENLGVPDTAKNRKIA
                     GELRTSVCFAIRMGSFDYAAQFPNSPNLKHFGLGKKEITIKALSEKWLELKKMEITAN
                     AMVRYKSAIRNCLDCLGGNKLASSITQEDVLIFRKELLTGYQLPGKNQRKPIKGRSVP
                     TVNSYMTIISGMFRFAANNGYIGKNPFSELPSLKRAKTPPDPLTKDEFARLIDACHHQ
                     QTKNFWSLAVYTGMRHGELCGLAWEDIDLEAGTLTVKRNYTQTKEFTLPKTDAGTNRV
                     IQLIQPAIDVLKNQATLTRLGKQYQIEVKLREYGRTSTHSCSFVFNPQILDRTGNSGV
                     HYAVTSINRTWESALKRAKIRHRKAYQSRHTYACWALTAGANPNFIANQMGHANAQMV
                     YNVYGAWMKDNNQSQVEVLNRKLCTYVPQVPQRLNKA"
     gene            complement(62046..62300)
                     /gene="xisR"
                     /locus_tag="ACHKIR_001126"
     CDS             complement(62046..62300)
                     /gene="xisR"
                     /locus_tag="ACHKIR_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFO9988893.1"
                     /translation="METIIYQITPSKWCTERTLIAATGLKSGTIARARKKSWLLGKEY
                     RHYAAEGEPGPFSECVYNIEEIMRWIENQKQPGAKNASSG"
     gene            complement(62308..62454)
                     /locus_tag="ACHKIR_001127"
     CDS             complement(62308..62454)
                     /locus_tag="ACHKIR_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9988894.1"
                     /translation="MNREFEIWIRLRYGYRYDLTRDAHGFYCREVVKRMFDVWCHCRG
                     LKIV"
     gene            complement(62475..62717)
                     /locus_tag="ACHKIR_001128"
     CDS             complement(62475..62717)
                     /locus_tag="ACHKIR_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000504369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9988895.1"
                     /translation="MGEMVDINDELVRTVITVDDGCDHTQRFIWMMNTRRNIRNKVFR
                     HEPKPVRVTKIRIEARKRRKYRPRKARAPAQPNELA"
     gene            complement(62710..>63534)
                     /locus_tag="ACHKIR_001129"
     CDS             complement(62710..>63534)
                     /locus_tag="ACHKIR_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9988896.1"
                     /translation="AFHSLATPPVILALISHIENLEKKLDEIVTSEISPKAWLNNLTG
                     EVCSIDAMPDARNDRETYTPLYPAWQRIAAADDNGRVAWMYTHKKAGYRVPTMTSWES
                     LAEERGGSLDKYIVSPLFTVPQIPPVRTEQDAYPDVQVSWDATRHYVNGWNACRQAML
                     TTAEKVLDVRIPEYIGRWLSAALHDQNSCEEMKNDIRRFLAEYHHVPSKYNGWRLVPD
                     KLTEQMKYRIVPLLATIKPRSVDEYDRSGENAQFLEWVWTQLMEATKFAYEGSVNG"
BASE COUNT        15243 a        15721 c        17008 g        15562 t
ORIGIN      
        1 gcaaaatgct gaacaacgca cggaacgtca gcgttccgat cgccaatggg cgcagcgttt
       61 tcgcacagaa ccgttaacgg cggtatttgc tgactggtat caacagcctg tttttgcctc
      121 actcaatgac gaacaacgcc gggagctggt tgcgctgcgc agcaacaata atggcgcaac
      181 acttgccgcc atgctggagg cgacttctct cgccgtccag cccgatttac gtgctaacct
      241 tagcgcccgc acatttgcgt tttattattt atgtggtgaa cgtgacagca aattccgcgc
      301 cctggcggcg gaactggctg ccgaatgcca tgtcattcct cgcgccggac ataacgcgca
      361 ccgggaaaat cccgctggcg taatcgcaag tctggcgcag atcttgcgtt tctgactaaa
      421 ggacacaata tgatttatcc tgatgaagca atgctttacg caccggttga atggcacgac
      481 tgctccgaag gttttgagga cattcgttat gaaaaatcca ccgacggtat cgcaaaaatc
      541 accattaatc gtccgcaggt gcgcaatgcc ttccgtcctc tgacggtaaa agagatgatc
      601 caggcactgg cagatgcgcg ttatgacgac aacatcggcg tgatcattct gactggcgca
      661 ggcgataaag cgttctgctc cggcggtgac cagaaagtgc gtggtgatta cggcggctat
      721 aaggatgatt ccggcgtaca tcacctgaac gtgctggact tccagcgtca gatccgtacc
      781 tgcccgaaac cggttgtcgc gatggtggct ggctactcca ttggtggcgg tcacgttctg
      841 cacatgatgt gcgacctgac tatcgcggca gataatgcca tcttcggtca gactggcccg
      901 aaagtcggtt ccttcgacgg cggctggggc gcttcctaca tggcgcgcat cgtcgggcag
      961 aaaaaagcgc gtgaaatctg gttcctgtgc cgtcagtacg acgcgaaaca ggcgctggat
     1021 atgggccttg taaataccgt ggtgccgctg gcggatctgg aaaaagaaac cgtgcgctgg
     1081 tgccgtgaaa tgctgcaaaa cagcccgatg gcgctgcgct gcctgaaagc tgcactgaac
     1141 gccgactgtg acggtcaggc gggtctgcag gagctggcgg gcaacgccac catgctgttc
     1201 tacatgacgg aagaaggtca ggaaggtcgc aacgctttca accagaaacg tcagcctgat
     1261 ttcagcaaat ttaaacggaa tccgtaatgc gtagcgcgca ggtataccgc tggcagatcc
     1321 ccatggacgc gggggtggtt ctgcgcgaca ggcggttaaa aacccgcgac gggctgtatg
     1381 tttgcctgcg tgagggcgag cgcgaagggt ggggggagat ctccccacta ccgggcttca
     1441 gccaggaaac ctgggaagag gcacaaagcg ggctgcttgc ctgggtaaat aactggctgg
     1501 caggtgatag cgagctaccg caaatgcctt ccgtcgcctt tggcgtaagc tgtgcattgg
     1561 cggagctggc agacacgctc ccgcaggcgg ccaattatcg tgcggcacca ctgtgtaatg
     1621 gcgatccgga cgatctgatc ctgaaacttg ccgatatgcc gggcgagaaa gtggcgaagg
     1681 tcaaagtggg attgtacgaa gccgtgcgcg acggcatggt ggtcaatctg ttgctggagg
     1741 caattccgga tctgcatttg cgtcttgacg cgaaccgcgc ctggacgccg ctgaaaggcc
     1801 agcagtttgc caaatacgtt aacccggatt atcgcgaccg catcgcgttt ctcgaagagc
     1861 cgtgcaaaac ccgcgatgat tcccgtgcgt ttgcccgtga aaccggcatt gccattgcct
     1921 gggatgaaag cctgcgcgag ccggattttg cctttgtggc tgaagagggc gtgcgcgcgg
     1981 tagttatcaa acccacgctc acgggcagcc tggagaaagt acgcgagcaa gtgcaggcgg
     2041 cgcatgcgct ggggctgacg gcggtgatca gttcttccat tgagtcgagc ttaggtctaa
     2101 cgcaactggc gcggattgct gcctggttaa cgccagacac cattccggga ctggacacgc
     2161 tggatctgat gcaggcgcag caggttcgtc gctggccggg gagcctgctg ccgttagtgg
     2221 acgttgatgc actggagcgg ctgttatgat cttctctgac tggccgtggc gtcactggcg
     2281 gcaagtgcgg ggagaagcca tcgccttacg tcttaatgac gagcaactca actggcgcga
     2341 gctttgtgct cgcgtcgatg aattagcctc cagctttgcg atgcagggtg tggttgaggg
     2401 cagcggcgtg atgttgcggg cgtggaatac gccgcaaacg ctgctggcct ggctggcgtt
     2461 actgcaatgc ggggcgcggg tgctgcccgt gaatccacag ctgccgcaac cgttacttga
     2521 ggaattgctc cccaatctta cgctgcaatt tgcgctggtg ccggatgggg aaaacacgtt
     2581 tccggcatta acgtcgctgc acattcagcg ggttgaaggc gcacatgccg ctacgtggca
     2641 gccgacgcgt ctgtgctcaa tgacgttgac ctcgggttct accgggctgc cgaaagccgc
     2701 tgtccatact tatcaggccc atcttgccag tgccgaaggg gtgttatcgt tgattccgtt
     2761 tggcgatcac gatgactggt tgctctcttt accgctgttt cacgtctccg gtcagggaat
     2821 tatgtggcgc tggttatacg ctggtgcgcg gatgacggtg cgtgataaac agccattgga
     2881 gcaaatgctg gcaggctgta ctcacgcttc actggtgcca acacaactct ggcgtttgct
     2941 ggttaaccgc agttccgttt ccctgaaagc ggtgttactt ggcggcgcgg ctatcccggt
     3001 cgagttgacg gaacaggcgc gcgagcaggg gattcgttgc ttttgcggct atggtctgac
     3061 cgagtttgcc tccacggtgt gtgcgaaaga agccgacggt ctggcagacg ttggttcgcc
     3121 gctgccgggt cgggaagtga aaatcgttaa tgatgaagtg tggctgcggg ctgctagtat
     3181 ggtagaaggt tactggcgta acgggcaact ggtttcactg gttaatgacg aaggctggta
     3241 cgctacgcgc gatcgcggtg agatgcataa tggcaagctg accattgtcg gacgtttaga
     3301 caatctattc ttcagtggcg gagagggtat tcagccggaa gaagtcgagc gcgtaattgc
     3361 tgcacatcct gcggttttgc aggtgtttat cgtccccgtt gccgacaagg agtttggtca
     3421 tcgaccggtg gcggtggtgg agtatgacca gcaaaccgtt gatcttgacg aatgggtgaa
     3481 agacaagctg gctcgttttc aacaaccggt gcgctggctg aatctgccgc cggagctgaa
     3541 aaacggcggt attaaaattt cacgtcaggc gctaaaagag tgggtgcaac gccagcaata
     3601 attaaccctc cgtttcactt ctcatttctg ccattccatt gccgttgata aagagtttat
     3661 ctgttaatct gtaaattgat gtgaaaactc ttagcaaaca ggataatgca atggaatggc
     3721 tggtcaaaaa atcgtgctgc aataaacaag ataacagaca tgtaattatg ctctgcgatg
     3781 ctggcggcgc gataaaaatg atcgccgaag tgaagagtga cttcgccgtg aaagtggggg
     3841 atttactctc gcctttgcag aatgcgcttt actgtattaa tcgtgaaaag ctgcacacgg
     3901 tgaaagtcct ttctgcaagc tgttatagcc ccgatgagtg ggaacggcag tgtaaagcgg
     3961 cagggaaaac tcagtaacgt tgttttgttg tgggcaggaa aatcagcatc aacccgctca
     4021 taatacaggc tactcccagt agtgctttca gcgaaaaagt cccttcccag ccgggaagta
     4081 ccattgaagc aatccatacc agcacataac tcatactgag caaggcatag gctttgctta
     4141 gtgcaagttt atgtagtgtt ttataccaac aaaaaacgga aagcagataa cccagcagcc
     4201 cgagtaacag tatccgggca tccaggccaa aagctaacaa cgccgcgata aaatcccata
     4261 agtgcgtcat cggcggcaga tgactcgccg caaaacccag gcttaattgc gcagcggagg
     4321 cgataatgac gctgaataat ccccacatca ggcccattac acggtactcc cgaggatcac
     4381 aatgccacca ataatgaacg ccaccccaca ccagtgacgc ggcgataccg gttcgtgcca
     4441 cagttttact gcagccagcg tcacccagac aaaattcaga cttaacatcg ggtaggcaat
     4501 gcctaccggt acgttctgca agaccagcag ccagagcacc atggcaagac caagacaagc
     4561 cagcgccagt cccagccaca gcacgatatg tttgcgccgt ttgtttatcg ccacaaagca
     4621 ggttgcctgt ttctgacaca actgcccggc aacgctaagc aagctggcaa agactaatgt
     4681 tagccagatc atttgggacg atactgaatc agcaccagac gctcctgacg atcgatggca
     4741 tctgcgggcg gaatggcgag actgttgata tcttcattgc ggtcaaccga aagcacgagt
     4801 gtaataatcc cctcctgacg atgttgatta agccagtttg cgaactcatc accgctgaca
     4861 aatctccctt tcgcatccgg ataattaagg ccgtatttca actcacctgt ctggcgatac
     4921 atgatgatgt catcgcgttg caggctccat gccagacctg cggcaacacc gacgctatca
     4981 gtaagaatat agcggcttgg ctgaagtgat tcttgtgtca tctcgacaaa aaattgcgga
     5041 tgttttcctt ccatgactct gtcaggaatt gaaaatccta ccagcaacgc cagccccagc
     5101 gggcaaagcg cggcaaaaga ccaggtcttt tcgacgtttg ttaaggtgta ccagccgaaa
     5161 aatgcccata gcgaaaaaat cgaccaggca caaaagactt tatagctctc gaaggtttgc
     5221 cacaccggcg tgttcattgc tccccagggg gagaccacaa atgtggcaat aatgccagtg
     5281 acgccaaaag cgatgttaat ccagccatta atccgcaacg ccagaggatt attttttgct
     5341 gccagcaaag cgtaatgcgc catcagcatt gccagaggag caaagcaaga aagaatatag
     5401 gtgggcaatt tgcctttggc gacggagaaa aacaacagcg gcattatcgt ccagctcaac
     5461 aaatagacgg ttgcggaatg cttgcggttt ttccagcctg cgtacagtgc tccggggagt
     5521 aatcccagcc acggcaggct accggcaatg atgaccggca agtagtacca gaacggagct
     5581 ctatgttggg catcatccat tgcaaagcgt tgaatatgct caacccagaa aaaatagtgc
     5641 cagaagtcag gctcccgctg cgctatcgcc agtccccagg ggagaaccgt cagtacgcaa
     5701 ctgatgaccg ccagccagcc gtaaataaag agatctttcc agcgtttttg cgttgcaacc
     5761 catggaagca cgcttaatac cggcacggca agggcgagaa aacctttggt catcaccccc
     5821 atgccgcaag tgattcccag cagtaaaaat cccgcgcttt tgcctttcca cgtctgtgcc
     5881 tgcattgcca gccagaagct gcacattccc gccaccagcc agaaggcaat aaacggatcg
     5941 agcacggcat aagtgccaat ggcatagaca ataaacaatg agagataaat cactgcggcg
     6001 agtagagcca gacgtttatc gcgccataag cgcagagtaa accaggtcac cagcgcggca
     6061 gttaacaggg tcgcaaagat aacgcctgcc cgcacaccaa agttattcgc gccaaatagc
     6121 cattgcccaa tgctgttaat ccagtatccg gcaatgggtt tttcgaaata acgtagccct
     6181 aacagatggg gcacaatcca gtcgccggat gccagcattt ctcgactgat ttccgcataa
     6241 cgcgtttcat cgggttgcca tagcagacgc gtgctgatcg gtaacaggta atagcaggca
     6301 ataaacgcga agaggccgat aaggtaacgt accgatttca tcagcgacta cccgcaattt
     6361 gttggcaacc cagccagcct tcacgtccgg caatatttcc gcgaacaact tgtccgagcg
     6421 gcaacatccc tgataacagt tcgcttaaag ggcaaaatgt cacgccttcc tgggctgccc
     6481 gtttaaggag atccacaaaa ttatgctgat aagcgcagcc ttcaacttct gcatgaatgg
     6541 tataaaccgg cgtgccttta tctcgcagga tgcgattgag taaccaaccg ttaaaatctt
     6601 ctgctttcac atcccgacca atcacttcat cccaggtggg taaggtcacc ggaatttgcg
     6661 cagtaccagg gtttcctgat tcgagcagtg gacgaaacgg catcgcccca cgacaatcgc
     6721 tgttgtagcg caaatggaac gcttcttttg cttcgatcac ctgctggtcg gcacgccagc
     6781 cagcggctgc ggagcaggtt accggttggc cgataatttc ttccagggtg cgaaggccgc
     6841 gtgcgatatc gtcgatcatt gtttgccgat cccaattacc gctatgggct tgccaggcat
     6901 ggtgatccca ggcgtgcagg ccgacttcgt gatgtttagc cgcttcacga atgatatcgg
     6961 cattggcatg accaatctct ttacccggcc aggccgtacc tgccagtaaa atatcccagc
     7021 cataaagcga tgccgcgttt gagcgcagca tcttccacaa aaactgtggc ttcaccagtc
     7081 gccagagatg gcggcccata ttgtccgggc cgacgctgaa aaaaatgctg gcctgaatat
     7141 tatgcttact caagatttcc agcagacgcg gcacgccttc acgggtgcca cgaaaggtat
     7201 cgacatcaat gcgtaagcct actttggtca tgatggttta tccgtaagat caacggtgcg
     7261 caggaagaaa tccagcgttt cgtcgatggt ttcctgcata tcaattttcg gttcccagtc
     7321 caggcagcgg cgggcattgc ggatgctcgg tttacgatgc tctacgtcct gatatccttt
     7381 gccatagtag ctgctacttt cgacaacacg aaagcctgca aacggcggga agtgatggcg
     7441 cagcggatgt ttttcgaagc tcgccagcag catctcgccc agttcctcaa tgctcgcttc
     7501 gttctcagga ttgccaatgt tgataatttc gccgtcgcag cgatttcccg cattttcgat
     7561 aatgcggtac aacgcctcga taccatcccg gatatcagta aagcagcgtt tttgttttcc
     7621 gccatcaatt agcttaattg gtgaaccttc taccagattg agaatgagtt gcgtaatagc
     7681 gcgggagctg ccaattcgcg ctgcattaag gttatccagt cgtggtccca tccagttaaa
     7741 cggccggaag agggtgaact gtaaaccctc tttttcgcca taggcccaga tcacccgatc
     7801 aagtaattgt tttgacaccg aataaatcca gcgtggttta ttcaccgggc cgacggttaa
     7861 attagaatgg tcctcatcga agtatttatc gctacacatc ccataaactt ctgaagttga
     7921 cgggaagatg attcgcttac ggtacttcac gcagtagcgg ataatgcgca gattctcttc
     7981 aaaatcgagt tcaaatacgc gcagcgggtt gcgggtatat tcaatcggcg tggctatcgc
     8041 caccagcggc aagacgacat cacatttttt gacgtgatac tcaatccatt cggaatgaat
     8101 actgatatcg ccttcgacaa agtgaaaatg cggatgattc agaaaacggc ttatcgcatc
     8161 gctgccaata tccagaccgt aaacttcata atgatcttcg cgcagcaggc gttctgtcag
     8221 atggttgcca ataaagccat tcaccccgag gatgagtacc cgggtacggc gtcgggcggc
     8281 acaggcaggc tggctattca agcgtgaacc ttgcaccagg cccagcgtct gcgctaattg
     8341 cgagccctgc atagtaatgc cgtcgcccgc ctgtccggtg acgatttcca gcgcgccatc
     8401 gccacaggca atcagcagtg gcgctacaga aatcacgctc cccggctgtg ctttgctggc
     8461 atgaggatga acacgcgacg accagacggt gaatttctga ttgccaacat agctgaaggc
     8521 acccggccac ggatcggcaa cggcacgtac catgttgtgc agtacggatg ccggcttatg
     8581 ccattcgagg aaactgtcat ccggcgttct gcgaccaaaa caggtagctt cgttttcgcg
     8641 ctgggcgatt tccagaatat taccgtgttt aatggtgggt aatgtctgct ccagtagctg
     8701 gcgcgcggca tgacataact tatgatgcaa cgtaatggcg atatcgtctg gcgcaatggc
     8761 aatgcgcagt tgcgccacaa tggccccggc atcggcacgt ttcaccattc ggtgcaatgt
     8821 aacgccagtt tccgtttcac cgttgaccag cacccagttc agcggcgcgc gaccacgata
     8881 ttttggtaac agcgaaccat gcagattaaa tgcacctgcg ggagcgagct gcaaaatctc
     8941 gtcgcaaata agatggcgat aataaaaaga gaaaatcacc tctggtgaga gttgggcaat
     9001 acgttccacc cacagcggat gattaacgtt atctggcgca taaaccggaa tgcctctttc
     9061 cgccgccaga cgagctaccg aaccataaaa ggctttttca ccgggattat cggtatgagt
     9121 aaaaatagcg ctaatttcgt aaccggcagc cagcagggct tcaataccga ggcatcccat
     9181 atcgtggtag gcaaaaacga cggttttcat tattcatttt ccttgctgga tggacggata
     9241 acttgctgaa caaaatagcg ggggcgggcg cggacatcgg tgtagatcct gccgatatat
     9301 tcaccgagta atcccatgcc gataaactga gcgccaataa aagtaaacag cacggcaaat
     9361 agcataaaga cgccttctgc cgcccattgt ggtccgaagg tcaggcgtaa aatcaccagc
     9421 agcaccgcaa tgctaaaacc tccaatcgca ataatgctgc cgagcagact cagcatacgt
     9481 agcggcgttg tggtaaggca ggtcaccagg tcgtacatca aattaatcag gcgcataaaa
     9541 ctgtatttgg attcaccaaa ctcacgctcg gcatggtgca ctggaatttc aatggcacgg
     9601 cgggcgaaga tattcgccag aatcgggata aaggtactgc gttcatggca gtgcaacatc
     9661 gcatcgacaa tatgacggcg ataggcgcgc agcatacaac cgtagtcacc catcgctttg
     9721 ccggtggtgc gctgaataag ccggttaatc atcttcgaag cggttttacg aaaccagctg
     9781 tcctggcggt tctggcgtac agtccctacc acgtcgtaac cttcatcggc ttttgccacc
     9841 agacggggga tttcctctgg cggattctgg agatcggcat cgagggtaat aattaagtcg
     9901 ccagtaacgt gactgaatcc cgccataatc gctgaatgtt gcccgtaatt gcggttaagc
     9961 aaaatagaca caatatggct gttctccgct tgtgaggctt cgaccagcat atgcgcggaa
    10021 ttatcgctac tgccgtcatc aatcagcagg atttcatact ctttccccag tgattcacag
    10081 gctgcggtgg tgcgcctgat taattccggt aagctttcct gctcgttata aacgggaata
    10141 accaccgaga ctttcttaac agggtggatt tcaaacatgg cttattgtcc tgcgagttgc
    10201 tgaagggctg tgatgacgcg gtcggcatcg gcggtggtca tatccgggaa cagcggcaac
    10261 gaacagatgc gttcgctatt ccattcggta ttcggtaacg acagcgtggg aaaacgctcg
    10321 cgataatatt tttgtgtgtg agcggcgcgg aaatgtaacc cggtaccaat gcctctttct
    10381 tttaacgctt ccatcaacgc atcgcgactg ataccacaac gttgttcatc gacacgaata
    10441 ataaacagat gccaggcgtg aacgtgtgac caggcgggaa ggcttaatgg ctgaaagggg
    10501 agagctgcca gtgcttgctg atattgctgg gcaatttcgc gccgacgggt gttgaggtgc
    10561 tctaatttga ctaactgtgt cagggcaatc gcggcgttaa tatcggtcag attgtactta
    10621 tagcccggcg ttaagacttc agcctgcggt gcacggcccc aggtatgtct gtcataggca
    10681 tcgacgccca gaccgtgaaa ttttagcatc cgtagctggc gggcaaggtt ttcattatct
    10741 gtaacaatca ggccaccttc cgcacaggta atatttttga tggcgtgaaa cgaaaaaata
    10801 gcggtacttt ttgcgccaat atgtcgccct ttgtaatacg tacctacggc atgggcagca
    10861 tcttcgataa ctgcgatgcc gtaacgttcg ccaatggcgc gaatggcgtc aatatctgct
    10921 ggcgcaccgg cataatgcac cggaatgatg gctttagtgc gtggcgtaat ggctgactcg
    10981 atcgcttcag gcgtgaccat cagcgtatcg cggtcgacat ccaccattac cggcgttgca
    11041 cccaacaagg agatcatatt gagggttgaa acccaggtca gggaaggcgt aatcacttca
    11101 tcgcccttgc caatttccaa cgccattagc gtgatatgca ttccggcggt ggctgaactg
    11161 accgcgatgg catgctgatt tcccgtcaac tggcaaaagg cttgttcaag cgcctgattc
    11221 ttcggaccgg ttgtgatcca accggattcg agaacctctt taactgctgc gagttcctcc
    11281 acgcccattg ctggtcgcga aaaaggcaaa aattctgaca ttgcttttcc ttccgccatt
    11341 gaatacctgt ccacttatat ttgctataaa gagtgttgtg tatattttgc catttggagc
    11401 gaaattctaa ggatagaata ttaacttaac cttaagaaac taatattaga cgtaaatatt
    11461 gaaattttta tattttttct tatttaggct ttgcatttgg caaaattttg aggcattttg
    11521 ccggcatcgt aggattttta atattacacc aactgctaat tatcgccaga aatgtcgctc
    11581 aatttcacgg taattgtctg gttgcgcttg tctataggtg gagtttacgt gttagctttt
    11641 tgccgctctt cgttgaagtc aaaaaaatat ttcatcattt tactggcgct cgctgcaatt
    11701 gccggactgg gtactcatgc cgcctggagt agcaatggtt tgccacgtat cgacaataaa
    11761 acgctggcca gactggcaca gcagcatccg gttgtcgttt tgtttcgcca tgctgaacgt
    11821 tgcgatcgtt caaccaatca atgcttgtca gataaaacag gtattacggt caaaggtacc
    11881 caggatgccc gtgaactggg caacgctttt agtgctgata tccctgattt cgatctctat
    11941 tccagtaata ccgtccggac cattcagtcg gcgacctggt tttcagcggg taaaaagctg
    12001 acggtagata aacgacttct tcaatgcggt aatgagattt atagtgcaat taaagactta
    12061 caacgcaaag cgcctgataa aaatatcgtt atttttaccc ataatcattg cctgacatat
    12121 attgctaaag ataagcgtga tgcgacattt aaacctgatt atctggatgg tttagtcatg
    12181 catgtggaaa aaggcaaagt ttatctggat ggggaattcg ttaaccacta aattttcaat
    12241 ctgacagcca gtgatggctg tcatcattat tacagaagac ctttcaaacg taacgttttt
    12301 cgggtggcga cattcaaatc atagtgcgcc agatcttcag gttttaccca cgcgtagtct
    12361 tgaaactctt cgtttatttt cacttctcgg ttggcagaaa cgcagtcaaa aatcaggtaa
    12421 atcatataaa tctcttcctt gcgaccatct gcatacgtct tggtgcgaat atcatcgctg
    12481 aaggtccacg gcgtgatttc tgtcaaaagc agctgttctc ccagctcttc gcgaatttcg
    12541 cggcgtagtg cctcttcaat tcgttcgccg ggctccacgc cgccacccga aagcgcccat
    12601 tgaccgggga aaacgccgcg atcgtcggcc attttacaca gcaaataagc accatcattt
    12661 tgaatcaaag ggcatacaat agtccgttgt cgcacagggg tattccttat aaataaaaag
    12721 aaaccatgag aatacggtaa ctcagaaata tgtgaaagtg acagtaatct cttttgcgat
    12781 gataactgca cttattatta tttttgtgtg tatcggtaaa ctgtgtttat tttttcggcg
    12841 tttaattatt taagaagtat ttagttacaa taagtgacta gatccctttt ttcgctcatt
    12901 gaatcctctg cgccaggcag tacaatcccg cagctttggg aattttgatt aattgcttgt
    12961 gggaaagggg ataatgaaaa aaattgcgct agcaggtctg gccggaatgc tgttggtttc
    13021 tgcatcggtc aatgcaatga gcatcagcgg ccaggcgggt aaagaataca ccaatattgg
    13081 tgtcggtttt gggactgaat cgacgggcct ggcgttaagc ggtaactgga cacataacga
    13141 cgacgacggt gacgtcgcgg gcgtggggct ggggttgaat ctgcctctcg ggccgttaat
    13201 ggcgaccgtt ggcggaaaag gcgtttacac caacccgaat tacggcgatg aaggttatgc
    13261 cgcagcggta ggaggtggtt tgcagtggaa aattggcaac agcttccgct tgtttggcga
    13321 gtattactac tctccggatt cgctctccag cggtattcaa agttatgagg aagcgaatgc
    13381 tggcgcgcgt tacaccatta tgcgtccagt cagtattgag gcgggttatc gctacctgaa
    13441 cctttctggt aaagacggta accgcgacaa cgcagtggct gacggcccgt atgtgggggt
    13501 taacgccagt ttttgatccc ttccggcgcg gcattctgcc gcgccattca gtttcttact
    13561 acgccttatc tccctttgcg ttatagtgat tcgataacaa aagcaggaga taaccatgtt
    13621 aaaagtggaa atgttatcca ccggggatga agtgttacac gggcaaatcg ttgacactaa
    13681 cgctgcctgg ctggccgatt ttttctttca tcaggggttg cctttaactc gccgcaatac
    13741 ggtgggggat aaccttgatg acttagtgac cattcttcgc gaacggagtc agcacgccga
    13801 tgtgctgatc gttaacggcg ggctggggcc gaccagtgat gatttaagcg cactcgccgc
    13861 tgcgacagca aaaggtgaag gcctggtgct gcatgaggcc tggctcaaag agatggaacg
    13921 ctatttccac gaacgtggac gagtaatggc accgagcaac cgtaaacaag cggagctgcc
    13981 tgccagtgct gaatttatca ataacccggt aggcaccgcc tgtggttttg ccgtgcagct
    14041 taatcgttgc ctgatgttct ttacccccgg cgtaccgtca gaatttaaag tgatggtcga
    14101 gcacgaaatc ctgccgcgcc tgcgcgagcg tttttcttta ccgcagccgc cggtttgtct
    14161 gcgtttgact acatttggtc gttcggaaag cgatctggca caaagcctgg acaccctgca
    14221 actgccgccg ggcgtaacaa tgggctatcg ctcttctatg ccgattatcg aactgaaact
    14281 caccggaccg gcaagcgaga aacaggcgat ggaaaaactg tggctggacg tcaaacgtgt
    14341 tgccggacag agcgtgattt tcgaaggtac cgaaggactg ccctcgcaga tcagccgcga
    14401 attgcaaacc cgccagttca gcctgacgtt gagcgagcaa ttcacaggtg gtttattggc
    14461 tttgcaactt tctcgcgcag gtgctccatt gctggcgtgt gaagtggttc cttcacagga
    14521 ggaaaccctg gcgcaaactg cgcactggat tacagaacgg cgggccaacc attttgccgg
    14581 gctggcactg gctgtttcgg gtttcgagaa cgagcatctc aactttgcgc tagccacgcc
    14641 agatggcact ttcgctctgc gtgtgcgttt cagcactaca cgctacagcc tggctatccg
    14701 tcaggaagtg tgcgcaatga tggcactgaa tatgctgcgc cgttggttaa acggccagga
    14761 catcgccagt gagcatggct ggattgaggt tgttgagtcc atgaccttat ctgtctgatt
    14821 ccgccactct gtgccggttt actgcaccgg cacattcccc ctccggcaaa atttgtccaa
    14881 cctgttgtac tcaataagaa aaaatccgtg cgctaattca caaatttgtg cgtaatttct
    14941 ccagtccgct ggacgaggag cgggatttga tgagcttcac aattttactc cacctgagca
    15001 acgagactga ttccagtatc taagactgga gtccagtatg ttggaatcaa gcaaagttcc
    15061 cgccctgaca cgggctattg atattctcaa tttgattgcc cgcattggcc cctgtagtgc
    15121 tgcgacgatc attgacacat tggggatccc taaaagcacg gcctatttgc tgcttaatga
    15181 actcaggcgt cagcgttttc ttagtcttga tcaccaggaa aatttttgtt tgtggaccag
    15241 gctggtggag ctttccggtc atgcgttaag caagatggat ctccgggaac tggctcgacc
    15301 gcgtttgacg caactgatgg ataccaccgg gctgttatgt cacctgggga ttattgataa
    15361 cggcagcgct tattacattt tgaaagtgga gtcatcagcc actatcagcg tgcgttccca
    15421 tgaaggaaaa agcctttcgc tttatcgctc cggaataggt aaatgcctgc ttgcctggca
    15481 acctgcagct gtacagcaaa gtattatcga agggctgatc tgggagcagg cgacacccac
    15541 aacgattact catccacagc aacttcacga agaactggcg cgtattcgcc gtcagggctg
    15601 gagctatgac aacggcgaag attacgccga tgtccgttgt gtcgctgcgc cggtgtttaa
    15661 cgccaataac gagcttaccg ctgcgatctc ggtggtcggt acccgcttac aaatcaatga
    15721 agaataccgt gattatctgg cgggcaaggc cattgcctgc gccagggata tttcacgtct
    15781 gttgggatgg aaaagtccct ttgacttaca ggcctcataa caatggagaa cagcatgacc
    15841 ctaccaaaaa tcaaacaggt tcgcgcctgg tttactggcg gtgcgacagc agaaaaaggc
    15901 gctggcggcg gtgattatca cgaccagggg gcaaatcact ggattgatga tcatattgcc
    15961 accccgatga gtaaataccg cgattacgag cagtcacgcc agtcatttgg cattaacgtt
    16021 cttggcacgt tgattgttga agtcgaagca gaaaacggcc agaccggatt cgccgtttcg
    16081 acagccggtg aaatgggctg ttttattgtc gaaaaacacc ttaaccgttt cattgagggt
    16141 aaatgtgtca gtgatatcaa actgatccac gatcaaatgc tcaatgccac cctgtattac
    16201 tccggctctg gtggcctggt gatgaacacg atttcctgtg tcgatctggc tctgtgggat
    16261 ctgttcggca aagtggtcgg gcttccggtt tataaacttt taggcggcgc agttcgtgat
    16321 gagattcagt tctacgccac aggtgcgcgt ccggatctgg caaaagagat gggctttatc
    16381 ggtggcaaaa tgccgacgca ctgggggcca catgatggcg atgcggggat ccgcaaagat
    16441 gccgctatgg tcgcggatat gcgtgaaaaa tgcggtgagg atttctggtt aatgctcgac
    16501 tgctggatga gtcaggacgt gaactatgcc accaaactgg cccacgcttg cgcgccctat
    16561 aacctgaaat ggatcgaaga gtgcctgccg ccacagcagt atgaaggtta tcgcgaactg
    16621 aaacgcaacg cgccagtcgg aatgatggtc accagcggtg agcatcacgg cacactgcaa
    16681 tctttccgta cgctttcaga aaccggtatc gacattatgc agccggatgt tggctggtgc
    16741 ggtggcttaa ccacgctggt ggaaattgcc gcaatcgcca aatccagagg gcaactggtg
    16801 gtgccgcacg gttcgtctgt ttactctcac catgcggtga tcagcttcac caatacgcca
    16861 ttcagcgaat tcctgatgac cagcccggat tgttcaacga tgcgtccgca atttgatccg
    16921 attctgctta atgagccagt gccggtgaat ggtcgtattc ataaatcagt gcttgataaa
    16981 cccggtttcg gcgtcgaact caatcgtgac tgcaatttga aacgccccta caaccactaa
    17041 tcactccggg cgttgctgtg acaacggtaa cgcccacata ttctaaggag ctttttatga
    17101 gcaccgcttt gcttgacgcc gtggtgaaga aaaaccgtgc gcgtttaatt ccgtttatgt
    17161 tggcgctgta tgtgctggcg tttctcgacc gttcgaatat cggttttgcc aaacagacct
    17221 accagattga taccgggctg agtaatgaag cttatgcgct gggagcaggc attttctttg
    17281 tggtatatgc gtttctaggt gttccggcga atcttttgat gcgcaaactg ggggccagaa
    17341 cctggattgg tacgacaaca ctgctgtggg gatttctttc ggcagccatg gcatgggccg
    17401 atactgaagc gaaatttctg atcgttcgca ctctgcttgg tgctgcggag gccgggttct
    17461 tccctggtat gatttatctc acctcgcaat ggtttccgca gcgtaatcgc gccagcatta
    17521 tggggctgtt ctatatgggc gcaccgttgg cgttaacact gggatcaccg ctttccggcg
    17581 cgctgctgga gatgcatgga tttatgggac atcccggctg gttctggatg tttgtgattg
    17641 aaggattgct ggcagtcggc gctggggtat tcacattctt ttggcttgat gacacaccgg
    17701 agcaggcacg ttttctgagt aaagaagaaa aaacattgct tatcaatcaa ctggcaagtg
    17761 aagaacaaca gaaagtgact tctcgactga gcgatgcgct gcgtaatggg cgagtctggc
    17821 aactggcgat tatctacctg accattcagg tggcggttta cggattaatt ttcttcctgc
    17881 cgacccaggt tgctgcattg ctggggacaa aagtgggctt tacggcgtcg gtggtcaccg
    17941 ccattccgtg ggttgcggcc ttgtttggga cctggcttat tccgcgctat tccgataaaa
    18001 ccggcgaacg gcgtaatgtc gcagcgctga cattactggc ggcaggcatt ggtattggtc
    18061 tctccgggct gctttctcca gtactggcga tcgtagcgct gtgtgttgca gccatcgggt
    18121 ttattgccgt gcagccggtg ttctggacga tgccgacaca actgctttcc ggtacggcgc
    18181 tggcggcggg gattggtttt gtaaacctgt ttggtgcagt gggtgggttt attgccccga
    18241 tcctgcgcgt gaaagcagaa acgttgtttg ccagcgatgc ggcgggatta ctgacgctgg
    18301 cagcggtggc ggtcatcggt tcgctgatta ttttcactct gcgtgtaaat cgcactgttg
    18361 cgcagaccga cgtggcacat cattaatagg ttaaggaaca cgatgaacgc attattaacc
    18421 aatcccttta aagaacgttt acgcaagggc gaagtgcaaa ttgggctgtg gttaagctca
    18481 acgactgcct atatggcaga aattgccgct acttccggtt atgactggtt gctgattgac
    18541 ggggagcacg cgccaaacac cattcaggat ctttatcacc agctacaggc ggtagcgccc
    18601 tatgccagcc aacccgtgat ccgtccggtg gaaggcagta agtcgctgat taaacaagtc
    18661 ctggatattg gcgcgcaaac tctactgatc ccgatggtcg atactgccga tcaggcacgt
    18721 caggtggtgt ctgccacgcg ctatcctccc tacggtgagc gtggtgtcgg ggccagtgtg
    18781 gcacgggctg ctcgctgggg acgcattgag aattacatgg cgcacgtcaa cgattcgctt
    18841 tgtctgttgg tgcaggtgga aagtaaaacg gcactggata acctggacga aatcctcgac
    18901 gtcgaaggga ttgatggcgt gtttattgga cctgcggatc tttctgcgtc gttgggctac
    18961 ccggataacg ccgggcaccc ggaagtgcag cgaattattg aaaccagcat tcggcggatc
    19021 cgtgctgcgg ataaagcggc tggttttctg gctgtggctc ctgatatggc gcagcaatgc
    19081 ctggcgtggg gagcgaactt tgtcgctgtt ggcgttgaca cgatgctcta cagcgatgcc
    19141 ctggatcaac gactggcgat gtttaaatca ggcaaaaatg ggccacgcat aaaaggtaat
    19201 tattgatatc aaaggcccat gggggtcggc tgtgggcctg tgttaattag tggttattcg
    19261 ctgccagatc ggcttcgctt agctgggtgg ctgcgagcac caggtcacga tcaatgccct
    19321 gtaccagcat gcgcaaagca attttaatgg cttgtttacg cataccttcc tgcaaacctt
    19381 ctaatttacc ctcctgccag ccttcaagct taccttcctg ccagccaatc tgatgccctt
    19441 cctgcctcag ccgttcagca atagtcatca atctctcctt ttaatgacag ttcggaatat
    19501 cagcatcagt gagttgggtt gtcgccagca caatctcacg ttcaaagccc tgctccagca
    19561 tgcgcaaggc aattttaatg gcttgttcat gcatgccttc ctgcaaacca tctaatttac
    19621 cttcttgcaa accttctaat ttaccttcct gccaaccttc aattttacct tcctgccagc
    19681 cttcaatctt accttcctgc cagccaattt gatgcccttc ctgccgcagc cgttcagcaa
    19741 tagtcattaa tctctccttt tgtagtggtg aacgttcggc aatctcctca ataaaacggg
    19801 tgaaacggga ggtatcgccg cattgcagca gataattaaa cagcgtttgt agctggctgt
    19861 cattagtgaa gcctctaacc aaaagcgtgg tgatcctgtc gaccataccg attaaatcgc
    19921 ggtcgcgaat atgcttttga atcagttcaa gcagagctat acgccgatgt tgcatgatct
    19981 catcgtcagg tacgatggtg atatccacca acggaaaagc ttcggtgtac aactgccggg
    20041 caagttgcgg atcgtcaaac tcatccagcc agttgagtga gtacgggtag ggcgaggttt
    20101 cgccatgata aaacagcaat ggcaccacca gcggaactct gtcatagcct ttatccaggt
    20161 gacgctgcat ggcggcagtg gcatagcgca ttagccgaaa agccatattc ttttctgcag
    20221 agctttgatg ttcaatcacg cagtagatat agccttcact gtcgctggtt tccacagacc
    20281 acaaaacatc cgagtagtaa gcgcgtaaac ttttttcaat aaagctggtg ggttccaggc
    20341 gtaaagtttg caggttgcaa agcttgcgca gcggctctgg tagatgtatt tccagaaaat
    20401 cccgagcggt ttcgggtgtg aacataaagg ttttaaacac cgcatcatgc ggggaggagg
    20461 ttgttgattc ggtcatggtt gtccgtcacc tgatatttat gatgacgcga cattacggtt
    20521 atcccaggcc tttgacatct gccagtttca gcctttgcga gacgttttca gaggaatttt
    20581 cactgcgttg caacgcatac attgggcacg gaatcgtgta cgcaaaaatg cccggtaaca
    20641 acatgcttac cgggcatcag aaaggagttt aagccagcgc ctgggccagc agcgtaatcg
    20701 gatgttcgca gcgaagactt gtggacatct caatctgcca tttgcaggtt tcgcagtcgg
    20761 taaccaccag atctgcgccg ctttcttcta tctgacggaa cagtggtgcg ccgatggctt
    20821 gtgaggtggg gtagttctct tttttgaaac cgtaagtacc tgcaataccg cagcactggg
    20881 aatccagcac cgttaactca agccccggga ttttacgcaa cagctccagg gtgtagagcg
    20941 tccagcccat tttttccata tggcacggag tgtgataaac cactttcagc ggcagcggtt
    21001 tcagcggcaa ggttttgcct tcgtccagct tgcgccacag ccagcgggtc gccagttcga
    21061 tgtgatcgcg caagcctttg ttgtcgacat tcagcacttc cggatattcg tcgcgcaggg
    21121 caaatgtaca ggttgaggag gtggcaatca ccggaatgcc ttttactccc actgcttcgc
    21181 ggatcgactc tacatttgtg attgcctgtt tacgtgcttt atcggtaaag ccgttggcga
    21241 tcagcggtac gccgcagcat ttttctttgc tgagcagttg tacaccggta cccattgcgt
    21301 tgagcacttt aattaaatct ttgcctaact gcggatggtt gtagttaacg aagcagccgt
    21361 gaaagaaagc cacctggtct ttatattgtg cctgctgggc cgccacgctg cgataccagc
    21421 gacggaacgt accgaaggag tatttcggta gcgtgcggcg atgatcgatt tttaacgccg
    21481 catcaagcag ctgccgcact ggtttcagcg aggtagcggt attgacgatt ggtgcgaatg
    21541 gcgtggaaac gctacccatc aggtcggtat gactcaacac aaaattgcgc agcgacgggc
    21601 gtgtggtgtc atatttcgcc cgcgcgcgct ggatgatatc gccaatcttc acatcggacg
    21661 ggcaggcgac ttcgcagcgt ttgcagttga tgcaatattt cagcgcttcg tcatacagtg
    21721 cgccatcttt cagacgcaga cgctcgccat ccggcccggc ttgttttggc cctggatagc
    21781 cgggattcac ccggctcacc gggcaggcgg tggtgcagac ggtgcactta atgcagtttt
    21841 cgaagctggt gtcgttcata gttggcctcc tgcgcgttgg gcaatctgtt gagcggcatg
    21901 taaagcactg acggcacaaa caccgccgcc gcatccctgg gcgatgggat caaatccgcc
    21961 cagcaccgaa ccgatggcaa acaggttctc aatggtttgc cctgcctgcg atgggcgtag
    22021 tgcctcatca gtggttacac cgaacagctg ccacggttgc ggcgcaaaaa aatcgccctt
    22081 ataccattca ccccgcgtgg cggtttgtag cacatcaagg ccgagaatcg gctcgcgaat
    22141 gccgtcacgt tctgccacca gtccgccgct aaagaaactg ccgctcgcaa gcaccgcaaa
    22201 tcgtgggcgc agcgggatat cggcgtgatt gcgcgtccag atttcattca ctacgccatt
    22261 tttacaggtc acttttttca cttcatcgcc tggcatccac acgccaccct ggcgcacaaa
    22321 ctggcgctgt aactggtttt gcagacgaat gcccagcacg gaaggcggca gcgttggcaa
    22381 aagcatcagt gaacaaggta gtttttcatt caaccaacgc cacagtttgt catcggccag
    22441 gccgaagcag gcgggcatca ggatcatttc gcaggtattg gcgacaggaa taagcgcatc
    22501 aagtaacagc gaccagttct cttcgttatc aaggaaacgg gcgatattca ccgcgcgaaa
    22561 ctcggtggcg ttgttgcgca gcacatccag ttccggcagc tctatttctg cggtttcaac
    22621 ggcaaggtcg agttcgcgca acgaagccgc cgcaagatgc gcctgaaaat ccatcaggcc
    22681 gcttatcccg accacacata ttttcttcgc gggcaacggc cagacaggaa cttctggcga
    22741 acttagccag gttgagcgca gagtgccgag cggggtaact cgctggtggg ccagttctac
    22801 gctgccctgt aattgcgcac cgctttcagc gattaatgcc tgcgcctggc aagcgagatc
    22861 gagtacgcgt tgtggcccga gaagagtgta aggatgggca ggtgcctgct gacgcaaagc
    22921 ttccagtcca ctgtgaatgt ctgtcaccgg ttgaccatcc ggcagatggc tcagcaaatc
    22981 cagcgatcca gatgagaaat ggagcgcgct ttgaccacgc gtgacaatgg cacagcgcag
    23041 gccgtgtttt tgcagttgca ggccacagag taatccggcg aggccgccgc ccataatgac
    23101 agtatcaaag cgcatctttc tgctccttct ccagaccaca cagtccctga taaacccagc
    23161 gggtaaattc gctttcgcgc agtgcatctc cccaggcgat gggttgcacg cctttccagc
    23221 gttcgttaag gaaggtggaa agttgctcga tagattgcgc ggacatcgtg acgttaaaac
    23281 gttgcagcag tccggcagcg cggcaggcgc agagttcgcc ctggcaggta cccatcccca
    23341 cacgggtacg acggcgtaaa tccagcaggc tattaacgtt taaattttct accgcgtact
    23401 gaacttcacc cgcagttacc gcttcgcact cacataccag gctacggtgc agacggcctt
    23461 cgctcagcca ggcgggtgtg cgatcgccat gacgataaac cgcagaaccg cgcagcgggg
    23521 caggcaggga gatgacttta cgcaaggtaa cttcagcggg ctcctgtgaa ccaggaagtg
    23581 ccagatcggc ggttgtacag gggcgcgtgt tgcccagctt gcgacatacc gcgtcggtag
    23641 cccattccgc catcagccga taggtcatca gtttgccgcc ggtgatggtg ataaatccgt
    23701 ccagaccatc gcgttcagca tggtcgagca gcacgatgcc acgactgacg ttacggccgc
    23761 tcgggtcgtc atcgctggca accagcggac gcacgccaga gtaagcccgc aaaatacgcg
    23821 ttttcgccat cacgggggcc agtttttccc cttcacgcag cagaatatca acttcttctg
    23881 ccgtcactcg attatcgtca atctcattgt aatcaatacg taaagaggtg gtgccaatta
    23941 gcgaaatggt atcgccaggc accagaatat cggcgtcgga aggtttgcgg cagcggttga
    24001 tcacatgctg attaatgcgg tgatccatga tcagcagcga tcctttcgcc gggaacatgc
    24061 gaatgcgcag atcggcatat tcggcaatgc gttgccccca gatcccagcg gcattaacca
    24121 cgacaggcgc atgaagggcc tgagtttcgc cggtgagatg gttacgtaca cgaacgccgc
    24181 acaccgtcgc accttcacga atcagccccg tgacttcatg agcggtaagg ataacggcac
    24241 cgtgttcttt ggcatccagc atgtttgctg cggtcagacg aaatggatca acggtgccat
    24301 ccggaacttt caccgcgcca atcagtgccg ggttaacggc aggttcgata atgcgcgctt
    24361 gctgcgggtc tatagcttct gcgctgatcc ctgcttcttc gcaggcgcgg ataaaagtgg
    24421 cctggaagga gaggtcatct tccggcaggg tgataaacag gccgttggtc ggttcaacgc
    24481 agtgacgtgc aatgcgtttc aggatctggt tttcactaat acattcgcgg gccgattccg
    24541 catcggttac cgcatagcgc gcaccgctgt gcagcaggcc gtggttacgc ccggtggcac
    24601 cggttgcgat gtcgtggcgc tcaaccaaaa tcacgcgcag tccgcgcagg gcacagtcgc
    24661 gggcaatccc ggctcccgtt gcgccgccgc caatgataat cacgtcactt gattgcgagt
    24721 cgcgagtttt cattgttatc cctctgaagt tcgtttttta ccatttagcc atagtaaaaa
    24781 catgaattgt ttgatttcgc gcatattcgc tcataattcg aaagtgaaac gtgatttcat
    24841 gcgtcattat gaacatttaa cccatcgaat ttaataatgt gtgcggtaat tcacatttaa
    24901 tttatgaatg ttttcttaac atcgcggcaa ctcaagaaac ggcaggttct cccactgaat
    24961 caggctgtta atcataaata agaccacggg ccacggaggc tatcaatgtt gagtattttt
    25021 aaaccagcgc cacacaaagc gcgcttacct gccgcggaga tcgatccgac ttatcgtcga
    25081 ttgcgctggc aaattttcct ggggatattc tttggttatg ctgcttacta tttggttcgt
    25141 aagaactttg ccctcgctat gccttatctg gtcgagcagg gattctcacg cggtgattta
    25201 ggttttgccc tttcggggat ctcgattgct tatggatttt cgaaattcat catgggctcg
    25261 gtgtcggatc gctcgaatcc gcgcgttttc ctgcccgcag gtttgattct ggcggcggcg
    25321 gtgatgcttt ttatgggctt tgtgccgtgg gcgacgtcga gcattgcggt gatgtttgtg
    25381 ctgctgttcc tctgcggttg gttccagggg atgggatggc cgccgtgcgg tcgtactatg
    25441 gtgcactggt ggtcgcagaa agaacgtggc ggcattgtgt cagtgtggaa ctgtgcgcat
    25501 aacgtcggtg gtggtattcc gccgctgctg ttcctgctgg ggatggcctg gttcaatgac
    25561 tggcacgcgg cgctctatat gcccgctttc tgcgccattc tggtggcact gttcgccttt
    25621 gcgatgatgc gcgatacccc gcaatcctgt ggcctgccgc cgatcgaaga gtacaaaaat
    25681 gattatccgg acgactataa cgaaaaagcg gaacaggagc tgacggcgaa gcaaatcttc
    25741 atgcagtacg tgctgccgaa caaactgctg tggtacatcg ctatcgccaa cgtgttcgtt
    25801 tatctgctgc gttacggcat cctcgactgg tcaccgactt atctgaaaga ggtgaaacac
    25861 ttcgcgctgg ataaatcctc ctgggcctac ttcttctatg agtatgcggg cattccgggc
    25921 accctgctgt gcggctggat gtcggataaa gtcttccgtg gcaaccgtgg ggccaccggc
    25981 gtcttcttta tgacactggt gaccatcgcg actatcgttt actggatgaa cccggcaggt
    26041 aacccaaccg tagatatgat ttgtatgatt gttatcggct tcctgatcta cggccctgtg
    26101 atgctgatcg gtctgcatgc gctggaactg gcaccgaaaa aagcggcagg tacggcagcg
    26161 ggctttaccg ggctgtttgg ttacctgggc ggttcggtgg cggcgagcgc gattgttggc
    26221 tacacagtgg acttcttcgg ctgggatggc ggctttatgg taatgattgg cggcagcatt
    26281 ctggcggtta tcttgttgat tgttgtgatg attggcgaaa aacgtcgcca tcaggaatta
    26341 ctgcaaaaac gcaacggagg ctaatggcat gaaattgacg ctgaaaaacc ttagcatggc
    26401 gatcatgatg agtggaatga tcatgggaag cagtgcaatg gcggcggaca gcaacgaaaa
    26461 aatagtcatc gcccatcgcg gtgccagtgg atatttgccg gagcatacgc tgccagcaaa
    26521 agcgatggcg tatgcgcagg gagcggatta tctggaacaa gatttggtga tgaccaaaga
    26581 cgaccatctg gttgtcctgc atgaccatta tctcgatcgt gttactgatg ttgccgatcg
    26641 tttcccggat cgggcgcgca aagacggtcg ttactacgcg atagatttca cgctcgatga
    26701 aattaagtca ctgaaattta ccgaaggttt cgatattgaa aatggtaaga aagtacagac
    26761 ttatccgggg cgtttcccga tgggtaaatc tgacttccgg gtacacactt tcgaggaaga
    26821 gattgaattt gttcaggggt taaatcactc taccgggaaa aatatcggta tctatccaga
    26881 aattaaagcg ccgtggttcc atcatcagga agggaaggat attgcggcaa aaacgctgga
    26941 agtgctgaag aaatatggtt acagcggtaa ggacgacaaa gtttatttgc aatgttttga
    27001 tgccgatgaa ctgaagcgta ttaagaatga gctggaacct aaaatgggca tggacctcaa
    27061 tctggtacag ctgattgcct ataccgactg gaacgaaact cagcagaaac agccggacgg
    27121 aagctgggtt aattacaact acgactggat gtttaagccg ggggccatga aacaggtggc
    27181 ggaatatgct gatggtattg gtccggatta tcatatgttg attgaggaga catcgcagcc
    27241 aggtaatatc aaactcactg gcatggtgca agatgctcag cagaacaagc tggtagtgca
    27301 tccttatacc gtgcggtcag ataaactgcc tgaatacact actgatgtga atcagttata
    27361 tgatgctctg tataacaaag cgggtgtaaa tgggctattt actgatttcc ctgataaggc
    27421 agtaaaattc cttaataaag agtaaattaa ctaccggatg gcacttatgt gctatccgat
    27481 accactcttc attaaagtct gtgggttatc cttaattgca attattttga gcgagatgac
    27541 ttgttcccgg cataagatac tcgtcaatac aacacgtatt attaagattt tcctcagcta
    27601 aacatctata gtcattgtcg ttaataaaaa acgacaggcg atagggtatg gcagtttcag
    27661 caaagcacga cgaatttaat cactggtggg caacagaggg cgactgggtt gaagagccta
    27721 actatcgccg taatggtatg agcggggtgc aatgcgtcga gcgcaacggc aaaaagctat
    27781 atgtaaagcg catgacgcat catctgtttc attccgtacg ttatccgttc ggccgaccaa
    27841 cgattgtccg tgaggttgcg gtaattaaag aactggaacg ggccggtgtc atcgtgccga
    27901 agatcgtttt tggtgaagcg gtgaaaattg agggggaatg gcgcgcgttg ctggtgactg
    27961 aagatatggc ggggttcatc agcattgctg actggtatgc ccagcatgca gtaacgccct
    28021 attctgacga agtacggcaa gccatgttga aagcagtggc gctggcgttt aagaaaatgc
    28081 atagcgttaa tcgtcagcat ggctgttgct atgttcgcca tatctatgtg aaaacagaag
    28141 gtaagtcaga agctggtttt cttgatctgg agaaaagtcg tcgccgcttg cgtcgcgata
    28201 aagcgattaa tcatgacttc cgccagctgg aaaaatatct ggagccgatc cctaaagcgg
    28261 actgggagca ggtgaaagcg tattactacg ccatgtaatc gttgtaggcc agataatacg
    28321 cgtaagcgtc gtatctggcg ctagtacccg atcacaactc gatttcaata tcgccttttg
    28381 cccggcaaca acagggcaaa atttcccccg gctgaataaa ggctaacggt tcggcaatcc
    28441 agtccacctg gcctgcaaca aggcgcgtgc gacaggaacc gcagtaacct tcgcggcact
    28501 ggtactcaac cgccacattg tgggattcca gcgccgccag aagggaaggg tgttcatcct
    28561 ggcacagcag ttgtgtgcca gtgatgcgca gggtaacgcg agccatcaga gctggaagtt
    28621 actcaaatcg tcggtgtcca cttccgagtc aatctgcccg accagataag aactgacttc
    28681 cacttcctgc ggagcaacct gcacgttatc agacaccagc caggtattga tccacgggat
    28741 cgggttggag cgtgtctgga acggcagatc caaaccgact gcctgcatgc ggatattggt
    28801 gatgtattca acgtactggc agagaatgtc tttattcaga ccaatcatcg aaccgtcacg
    28861 gaacagataa tccgcccagt ctttctcttg ctgtgccgcc tggacaaaca ggtcatagca
    28921 ctcctgctta cattcttcgg caatttccgc catctccgga tcatccgcgc cgctgcgcag
    28981 cagattcagc atatgctgag taccggtcag gtgcagggct tcatcgcgag caatcaggcg
    29041 aataattttg gcgttacctt ccatcagttc gcgttctgca aaggcaaagg aacaagcaaa
    29101 gctgacgtag aagcggatcg cttccagcgc gttaacgctc atcaggcaga gatacagttt
    29161 tttcttcagc tcgcgcaggc taacggttac ggttttaccg ttaacggtgt gggtaccttc
    29221 gcccagcaga tgccagtagc tggtcatttc gatcagctca tcgtaatagc tggagatccc
    29281 ttccgcacgt ttctggatct gctcgttggt gacgatatcg tcaaacacaa cagacggatc
    29341 gttaacgata ttacgaatga tatgggtata agaacgagag tgaatggttt ctgagaacgc
    29401 ccaggtttcg acccaggttt ccagctcagg gatcgagatc agcggcagca gtgccacgtt
    29461 cgggctgcga ccctgaatgg agtccagcag cgtctgatat ttcaggttgc tgataaagat
    29521 gtgtttttcg tgttcaggta gtgcctggta atcgatacgg tcacgagaaa cgtcaacttc
    29581 ttccggacgc cagaagaaag agagctgctt ttcgatcagc ttttcgaaga tgtcatattt
    29641 ttgctgatcg tagcgagcca cgttaaccgg ctgaccaaag aacatcggtt ctttcagctg
    29701 atcatttttc gtctgtgaaa aggtggtata tgccatgaat gtgtcctgtt gggagtttaa
    29761 tgccggataa ggcattttac gccgcatccg gcatctcaat atcagatctt acatgcgccg
    29821 ctttcgcagc catcgtcctg gattgacggt accagatcgt cttgtgcgtc ttcagcaccg
    29881 tcacgggtgt tctgataata cagcgttttg accccgaatt tgtaggcggt gagcaggtct
    29941 ttcagcagtt gctgcatcgg cacttttcct gacgggaagc gtgacggatc gtagttggtg
    30001 ttagcagaga tcgactgatc gataaatttc tgcatgatac ccaccagttg caggtagcca
    30061 tcgttacccg gcatttccca cagcagttca taggcgtcgt gcagatgctc gtagtccggc
    30121 accacctggc gcagaatacc gtctttcgac gctttgatgc tgacgtaacc acgcggcggt
    30181 tcaataccgt tagtggcgtt agagatctgc gaagaggtct cggacggcat cagggcagaa
    30241 agcgtggagt tacgcaggcc gtgcgttttg attgactcac gcagtgcttc ccagtcgtaa
    30301 tgcagcggct cattagcgat ggcatccaga tctttcttat aggtatcgat cggcaggatc
    30361 cctttcgcgt aagtggtttc gttaaaccac gggcacgcgc cttgctcttt cgccagctca
    30421 ttagacgctt tcagcaggta atactgaatg gcttcgaagg ttttatgcgt caggttgttg
    30481 gcgctgccgt cggagtagcg tttaccgtgc ttcgccaggt agtaagcgaa gttgatcaca
    30541 ccaataccca gcgtacgacg acccatcgct ccacgtttgg cggccgggat cgggtaatcc
    30601 tgataatcca gcagcgcgtc gagtgcgcgt actgccagaa ttgccagctc ttccagttca
    30661 tccaggctgt taattgcgcc caggttgaaa gcagacagcg tacacagcgc gatttcaccg
    30721 ttctcgtcgt tgacgtcgtt cagtggtttg gtcggtaggg cgatttccag gcacaggtta
    30781 gactggcgca ctggtgcaat agccggatca aacgggctgt gggtgttgca gtggtctacg
    30841 ttctgaatat agatacgacc ggtagacgca cgttcctgca tcatcagcga gaacagctca
    30901 acggctttca cacgctgctt gcggatgctg tcgtctttct catatttggt gtacaggcgt
    30961 tcaaactctt cctgatcggc gaagaacgcg tcgtacagcc ccggtacgtc ggacgggctg
    31021 aacagggtga tatcttcacc tttcagcaga cgggtataca tcagtttgtt gatttgtacc
    31081 ccgtagtcca tatgacgcac gcggttgcct tcaaccccac ggttgttttt caacaccagc
    31141 aggctttcca cttccagatg ccacatcggg tagaacagtg ttgccgcacc gccgcgcaca
    31201 ccgccctgag agcaggattt caccgctgtc tggaaatgtt tgtaaaacgg aatacagccg
    31261 gtgtggaatg cttcgccacc gcgaatcggg ctacccaacg cacgaatacg cccggcgttg
    31321 atgccgatcc cggcacgctg ggaaacgtat ttaacaatcg cgctggaggt ggcgttgatg
    31381 gaatccaggc tgtcgccgca ctcgatcagt acgcaggagc tgaactgacg agtcggggta
    31441 cgcacgccgg acatgattgg cgtcggcagc gaaattttaa atgtggaaac cgcgtcgtaa
    31501 aaacgcttca cgtattgcag gcgcgtttca cgcgggtagt tcgagaacag gcacgcggca
    31561 accagaatat aaaggaactg ggcgctttcg tagatttcgc cggtcacgcg gttctgtacc
    31621 agatatttgc cttccagctg cttaacggca gcataagaga aggtcatatc acggtcgtga
    31681 tcgataaacg tgtccatctg cttgaactct tcttccgtgt agtcttccag cagatgatta
    31741 tcgtatttgc ccatctcgac cattttcacc acgtggtcgt acagcgcagg cggctcaaac
    31801 tggccgtagg cttttttacg caggtggaag atcgccaggc gcgcggcgag atactgataa
    31861 tccggcgcat cacgggagat caggtctgcg gcagccttga taatggtttc gtggatgtca
    31921 gaggtcttga taccgtcata aaactgaatg tgggagcgca gttcgacctg ggaaatcgaa
    31981 acgttatgca gtccttctgc cgcccaatcc agaacgcgat ggattttgtc gagattgatg
    32041 cgctctgtgc taccgtcgcg ctttgtcacc agcagattct gattcatgta tgtcgtacct
    32101 gtttttggaa ttctttccgc aatagtttca tgcagactac ctgtagtgag ggaagctgct
    32161 tagaacacta tatatagggg gtatgtttgg gattcactgc aagatagtgt gaaaatggcc
    32221 ctcttgcaag tgcataactt tgtggataac tcaggaagga aaaagtggct ttcgcgcacc
    32281 ttaggtcaga caaggtgtcc gggaaagtca atgggaagag aaaaatttgt taaaaataac
    32341 tgttcggtga tttcttgaac ggcagaatag gttggataaa ttgatataga tggtttatta
    32401 atctttttgc taatgattgc aaaaattgat ctaagcacga gtcatcacat aatctgatgt
    32461 atcagttgtg gtcaatatat aagcgaatgt aagcgattgt tgatgtcgaa atggttgttt
    32521 tctatgcggt tattgttaaa tactaagcca atttgttact tttgtattta ttataagaat
    32581 attatttatt gctttaattt agtttaaata taaccgattt accttaaagt tattaatttg
    32641 aattgtgatg catctaaatt aattatcaga taataacaaa atagttatta catcaggggc
    32701 agaatttaca aaagaaaaat accctatatc gcataatgcg attaaatacg ccatcttata
    32761 gaaaatgatg gttagatgaa tatgcggatt atctttctac gcaaggagta tttatcttta
    32821 ctcccgtcaa tgattgcatc tcttttctct ggtaacggtg ccgcagcggt catcgattca
    32881 tgccagggat atgatatcaa agcgaactgt caggccagca ggcaaagcct ttcgggcaat
    32941 atgcaggact ggagcatcgc ggatgggcaa tggttgattt tttcgggtat ggccaataat
    33001 gccagcggtg gcgcagtatt tttgcagcaa agtgctgaat ttacgatttc accacaaaat
    33061 gaaacaggga tgaccctgtt tgctgataac tcggttagtg gcgaatataa taatggtggg
    33121 gctatctttg ctaaagaaaa ctcaacgata aatcttgcga atgttatttt cgacagtaat
    33181 gttgcaggag gctatggcgg agcaatctat tctgccggta ccaatgatac cggtgccgcc
    33241 gatttacgtg tcactaacgc cgtatttcac aataacatcg ctaatgacgg caaaggcggt
    33301 gcaatttata ccatcaataa tgacgtctat ttaagtgatg atgcttttaa taataaccag
    33361 gcatatacat caacaagtta tagtgatggg gatggcggcg ctattgatgt tacggataat
    33421 agtacggata acacacatct ttcaggaaaa acgataatta ataatacctc ctttaccaat
    33481 aactatgcag aaggttatgg cggagcaatt tataccagca gcaccacatc cccgtatctt
    33541 attgatattt ctgttgatga taattatgac caaaacaatg gtgtgatgat tgatgaaaat
    33601 aacagcgcct cgggatatga ccattcggcg acggcagcgg cggggggctt tatgtatatc
    33661 ggtcatagtg tggctgaatt taacattgcc gccgataaaa cgctggtaat aggcaatacc
    33721 agcaacgatg gcgcgattga ttctcttgcc ggaacgggag tcatcgttaa agagggagcg
    33781 ggagagttag tcctcaatgc cgacaataat gcgtttaccg gcgagataag tatccagaat
    33841 ggcgaggtga cgttggggcg cagcgatgag ttaatgaatg tcggcgacac gcactgtcag
    33901 agcgatccgc aggattgctt tggtctgatg gtcggcagca ctgttcattc tgagtatcag
    33961 gcagaactga atgttggcaa tacacaacaa acgtttgtgc actcattaac cggttttgct
    34021 aatggcattt tgaatatcga cgcaggcggc aatgttaccg taaaccaggg cggtttttcc
    34081 ggctcgattc agggtgaagg acagttgacg gtagcgcaag atggcagcta tctcctgaca
    34141 ggggcgcagt cgatggcatt aaccggcgat attgtggtgg aagataacgc cgtattgtcg
    34201 ctagcgggga atcaggccga tttacgggca atgcagagcg atccgcaatc gatcgtgcta
    34261 aatggcggag tattggatct ctctgatttc accacctggg atggcgatag ctcatacaat
    34321 gacggtctgc aaatcagcgg cagcgggggg acagtgattg gcagtaatga tgtcgttgat
    34381 atcagcagtg gcgatgattt acatattggc ggcagtgatg ccagccagaa tggcgtttat
    34441 gtcgtcatta atgcggggga tcagcgcgta acgctggcga ataacaacgg ttatctcggt
    34501 aatacgcaaa tcgcctccgg tacgcttgaa gtcagcgata attcgcaatt gggggacaca
    34561 agttacaacc gttcagtgat cttcacagat ccccaacaac acagtgaaat ggatgtgacg
    34621 actgatgttg acactcgctc ggctacaaca ggtcagggca ggaatattga aatgcgcgct
    34681 gatggcgaaa tacacgttga ggatggcgta gatacgcaat ggggcgggtt gatggcggat
    34741 agtaccgggc aacaactgga gagcgtaagc acgttgacta aaagtggcgg cggtacgctg
    34801 gagttgaccg ccagcggcac tgcgacgtct gcggtacgtg tggaggacgg aacgctaaaa
    34861 ggtgaagcgg agaatattat tccttatgtt tcttcactgt gggtggggga agacggtgtt
    34921 tttgaaaccg ggcaaaatca ggatattcgc tcaatcgatg ccacctctgg cggcgatatc
    34981 gacataaccg atggtactgt gttgcgattg acgcagcagg atacgaacca ggtgctggat
    35041 gcctcgctgt ttagcggcga cggtacgttg gtgaatgcca ccgatggcgt gacgctgaca
    35101 ggcgagctta ataccaacct tgaaactgac agcctgactt atctttccga cgtgacggtt
    35161 aatggcaatc tgaccaatac gtccggtgcg attagcctgc aaaatggcgt cgctggcgac
    35221 acgctgacgg taaatggtga ttataccggc ggcggtacgc tactgctcga cagcgaatta
    35281 aacggcgatg actcggcaag tgaccaactg gtattgaacg gtaatactgc tggcaacacg
    35341 gccgtggtaa ttaaccccat tacgggtatt ggtgagccga catctacagg tattaaagtg
    35401 gttgatttcg cagctgatcc cacgcagttt caaaacaatg cgcagttccg tctggcagga
    35461 agcggctacg tcaatatggg ggcgtatgac tatacgctgg tggaagataa caacgactgg
    35521 tatctgcgat cgcaagaagt cactccgacg ccgccgcccg atccggaccc gactccagat
    35581 cctgatccca cgccggatcc cgacccaacc cccgaccctg gacctacgcc tgcttaccag
    35641 ccggtgttga atgccaaagt tggtggatat tttaataacc tacgtgcggc aaatcaggca
    35701 tttgtgatgg agcgacgcga tcaggctggt ggcgatggtc agacgctgaa tttacgcgtt
    35761 atcggagggc gttatcatta cacggcggca gggcagttgg ctcagcatga agacacttct
    35821 acggtgcagc ttagcggcaa cctgtttagc gggcactggg gtgatgatgg cgagtggatg
    35881 cttgggattg ttggtggcta cagcgataac cagggcgaca gccgctcgaa tatgaccgga
    35941 actcgcgccg ataaccagaa ccacggttat gccgttgggc tgacctcaag ctggtatcag
    36001 cacggtaatc agaagcaggg ggtctggctg gatagctggc tgcaatatgc gtggtttaac
    36061 aatgatgttt ccgaacaaga cgatggcgag gatcattacc actcgtcggg gattatcgcc
    36121 tcgctggaag ccggttatca gtggttaccg gggcatggtg tggtgattga accgcagacg
    36181 caggtgattt atcagggcgt gcagcaggat gattttaccg ccgctaacca tgcgcgcgtg
    36241 tcacaatcgc agggtgatga tattcagacg cggctgggtt tacacagcga atggagtact
    36301 gctgttggtg tcacgccaac gttagatctg aattattatc acgatcccca tgcgacggaa
    36361 attgaagagg atggcagcac tatcagtgac gatggggtga agcaacgggg tgaaataaaa
    36421 gtgggagtca cgggcaatat cagtcagcga gtgtccctgc gcggcagcgt ggcgtggcag
    36481 aaagggagtg atgattttgc ccagacggca gggtttttgt cgatgacggt gaaatggtaa
    36541 tcgctgtctg accggcactt ttgccggatg cggcgtaaac gccttatctg gcctacggga
    36601 gcatttgtag gcctgataag acgcggcaag cgtcgcatca ggcattggca cggttgtcgg
    36661 atgaatccaa acaacaacct cacttattct gcgtgtgcag catatagttc acatccacgc
    36721 cggggccgag cttaaaggaa ttagtgatcg ggttgtaatg cagcccggtc atatgccgct
    36781 cttttaaact ggtctgatcc acccagccca gcaattctgc cggtttaata aacttcttca
    36841 catcatgcgt gcctttgggc accatgcgca aaatatattc agcaccaacc accgccatca
    36901 gccatgactt gccgttgcgg ttaagcgtcg agaaaaagac atcgccgcct ggtttcacca
    36961 gttgcgcaca ggctctgacc actgactgcg gatcggggac gtgctccagc atctccatgc
    37021 aggtcactac atcatactgc ccggcatgtt ttgccgcgtg ctcttccacg gtttcctgta
    37081 cgtactctac ctgaatgccg ctttccagcg cgtgcaactt tgccacctgc aatggctcaa
    37141 agcccatatc cagaccggtc accgtcgcgc cttcgcgcgc catactctcg gccagaatgc
    37201 cgccgccaca accgacatcg agcacctttt tgccaaataa accgccagca cgctcggcaa
    37261 tatagcccag acgcagcggg ttaatgcggt gcagcggttt gaactcacct tcaagatccc
    37321 accagcggga ggcgacagct tcaaatttag cgatctcttc gtggtctacg ttatggttta
    37381 ccggcgattt ttcggcattc attggcactt ctactccgta attggcaaga caaacgagta
    37441 tatcaggcat tggaggtgaa taaagcgtat aggtttacct caaactgcgc ggctgtgtta
    37501 taatttgcga cctttgaatc cgggatacag tagagggata gcggttagat gagcgacctt
    37561 gcgagagaaa ttacaccggt caacattgag gaagagctga agagctccta tctggattat
    37621 gcgatgtcgg tcattgttgg ccgtgcgctg ccagatgtcc gagatggcct gaagccggta
    37681 caccgtcgcg tactttacgc catgaacgta ctaggcaatg actggaacaa agcctataaa
    37741 aaatctgccc gtgtcgttgg tgacgtaatc ggtaaatacc atccccatgg tgactcggcg
    37801 gtttatgaca cgatcgtccg tatggcgcag ccattctcgc tgcgttacat gctggtagac
    37861 ggtcagggta acttcggttc catcgacggc gactctgcgg cggcaatgcg ttatacggaa
    37921 atccgtctgg cgaaaattgc ccatgaactg atggccgatc tcgaaaaaga gacggtcgat
    37981 ttcgtagata actatgacgg cacggaaaaa attcccgacg tcatgccaac caaaattcct
    38041 aacctgctgg tgaacggttc ttccggtatc gccgtaggta tggcaaccaa catcccgccg
    38101 cacaacctga cggaagtcat caacggttgt ctggcgtata tcgatgatga agacatcagc
    38161 attgaagggc tgatggaaca catccccggg ccggacttcc cgacggcggc aatcattaac
    38221 ggtcgtcgcg gtattgaaga agcttaccgt accggtcgcg gcaaggtata tatccgcgcc
    38281 cgtgctgaag tggaagttga cgccaaaacc ggacgtgaaa ccattatcgt ccacgaaatt
    38341 ccgtatcagg taaacaaagc gcgcctgatc gagaagattg cggaactggt aaaagaaaaa
    38401 cgcgtggaag gcatcagcgc gctgcgtgac gagtctgaca aagacggtat gcgcatcgtg
    38461 attgaagtga aacgcgatgc ggtcggtgaa gttgtgctca acaacctcta ctcccagacc
    38521 cagttgcagg tttctttcgg tatcaacatg gtggcattgc accatggtca gccgaagatc
    38581 atgaacctga aagacatcat cgcggcgttt gttcgtcacc gccgcgaagt ggtgactcgt
    38641 cgtactattt tcgaactgcg taaagctcgc gatcgtgctc atatccttga agcattagcc
    38701 gtagcgctgg cgaacatcga cccgatcatc gaactgatcc gtcatgcgcc gacgcctgca
    38761 gaagcgaaaa ctgcgctggt ggctaatccg tggcagctgg gcaacgttgc cgccatgctc
    38821 gaacgtgcgg gcgatgatgc tgcgcgtccg gaatggctgg agccagagtt cggcgtgcgt
    38881 gatggtctgt actacctgac cgaacagcaa gctcaggcga ttctggatct gcgtttgcag
    38941 aaactgaccg gccttgagca tgaaaaactg ctcgacgaat acaaagagct gctggatcag
    39001 atcgcagaac tgctgcgtat tcttggcagt gccgatcgtc tgatggaagt gatccgcgaa
    39061 gagctggaac tggttcgtga acagttcggt gacaaacgtc gtactgaaat caccgctaac
    39121 agcgccgaca tcaacctgga agatctgatc atccaggaag atgtggtggt cacgctttct
    39181 caccagggtt acgtgaagta tcagccgctt tctgaatatg aagcgcagcg tcgtggcggg
    39241 aaaggtaaat ctgccgcacg tattaaagaa gaagacttta tcgaccgact gctggtggcg
    39301 aacacccacg accatattct gtgcttctcc agccgtggcc gcgtctattc gatgaaggtc
    39361 taccagttac cagaagccac tcgtggcgcg cgtggtcgtc cgatcgtcaa cctgctgccg
    39421 ctggagcagg acgaacgtat cactgcgatc ctgccggtga ccgagtttga agaaggcgtg
    39481 aaagtcttca tggcgaccgc taacggtact gtgaagaaaa ccgtcctcac cgagttcaac
    39541 cgtctgcgta ccgccggtaa agtggcgatc aaactggttg aaggcgatga gctgatcggc
    39601 gttgacctga ccagcggcga agacgaagta atgctgttct ccgccgaagg taaagtggtg
    39661 cgctttaaag agtcttctgt ccgtgcgatg ggctgcaaca ccaccggtgt tcgcggtatt
    39721 cgtttaggcg aaggcgataa agtcgtctct ctgatcgtgc ctcgtggcga tggcgcaatc
    39781 ctcaccgcaa cgcagaacgg ttatggtaaa cgtaccgcag tggcggaata cccgactaag
    39841 tcgcgtgcga cgaaaggggt tatctccatc aaggttaccg aacgtaacgg tttagtggtt
    39901 ggcgcggtgc aggtagatga ctgcgaccag atcatgatga tcaccgatgc cggtacgctg
    39961 gtacgtactc gcgtttcgga aatcagcatc gtaggccgta acacccaggg cgtgatcctc
    40021 atccgtactg cggaagatga aaacgtagtg ggtctgcaac gtgttgctga accggttgac
    40081 gaggaagatc tggataccat cgatggcagt gccgcggaag gggacgatga aatcgctccg
    40141 gaagtggacg ttgacgacga gccagaagaa gaataatttt acttcttcat gccaaaaggg
    40201 agctatctcc cttgtttgaa ttgaaaagtc caggctgcaa agtctgggct tttgtcgtat
    40261 tagggcgcgg taaagttttg ctgtgctcgt aaaaaatggc tggctttaca caaggaatgt
    40321 ggcaatgagt ggtgaaaaaa aggcgaaagg ctggcggttc tatggtcttg taggtttggg
    40381 cgcaatagta ctgctttccg ctggcgtctg ggcgttgcaa tatgctggca gtgggccaga
    40441 aaaaacgttg tcgccgctgg tggtgcacaa caatctgcaa atcgatctca atgagccgga
    40501 cctctttctc gacagcgact ctctgagcca gcttcccaaa gacctcctta ccattccgtt
    40561 tctccacgat gttctgagtg aagatttcgt tttctattat cagaatcatg ccgatcgtct
    40621 gggcattgaa ggcagcattc gccgcattgt ctatgaacac gatctcacgc tgaaagataa
    40681 gctcttttcg tcactcttag atcagcccgc gcaggcggcg ctatggcacg ataaacaagg
    40741 ccatctttca cattacatgg tgctgatcca gcgcagtggt ttaagcaaac tgttggagcc
    40801 attgttgttt gccgctacca gcgacagcca gttaagcaaa acggaaatca gtagtattaa
    40861 gctaaatagt gaaaccatcc cggtttatca gttgcgctat aacggcaata acgccctgat
    40921 gttcgcgact tatcaggaca agatgctggt gttttccagt acggatatgc tgtttaaaga
    40981 tgaccagcag gataccgaag ccacggcgat cgcaagtgat ttgttgagcg gtaaaaaacg
    41041 ctggcaagcc agctttggct tggaagagcg tgctgctgaa aaaacgccag tacgccagcg
    41101 tattgttgtc agcgccaggt tgctggggtt tggctaccag cgattaatgc cttcttttgc
    41161 tggcgtacgc ttcgaaatga gcaacgacgg ctggcatagc tttgtggcgt taaatgatga
    41221 atccgccagc gtagatgcca gtttcgattt tacgccggta tggaacagta tgcctgccgg
    41281 ggccagcttc tgtgtggcgg tgccgtattc gcatggtatt gccgaagaga tgctttcgca
    41341 catcagccag gaaaacgaca agctgaacgg ggcgttagat ggtgcagccg ggctgtgctg
    41401 gtatgaagac tcaaaattac aaaccccgct atttgttggt caatttgatg gctcagccga
    41461 acaggcacaa ctgcctggga aactgtttac gcaaaatatt ggtgcgcacg aaagcaaagc
    41521 gccagaaggt gttttgccgg taaaccagac tcagcagggc gaagcgcaaa tctggcgtcg
    41581 cgaagtgagt tcccgatacg gccagtatcc gaaagcgcag gcggtgcaac ccgatcaatt
    41641 aatgtcggat tattttttcc gcgtgtcgct ggcgatgcaa aacaagacga tgcttttctc
    41701 cctcgatgac acgctggtta ataacgcgct gcaaacactg aataaaaccc gcccggcaat
    41761 ggtggatgtg atacccactg acggcatcgt tccgctctat atcaatccac aaggcgtggc
    41821 gaaactgctg cgtaacgaaa cgctgaccag tttgccgaag aatctcgaac cggtttttta
    41881 taacgccgca caaactttat taatgccgaa gctggacgct ttatctcaac aaccgcgtta
    41941 tgtcatgaag ctggcccaga tggaacccgg tgccgcctgg cagtggctac ccataacctg
    42001 gcaaccgcta tgagacacgg gctgctggcg ctgatttgct ggctgtgttg tgttgttacc
    42061 catagcgaaa tgctgaatgt cgaacaatcc gggctgtttc gcgcctggtt tgtacgcatt
    42121 gcacaggaac agctccgcca gggtccaagt ccacgctggt atcagcagga ttgtgcgggc
    42181 ctggtgcgat ttgcagcgaa cgagacgctg aaagttcacg acagtaaatg gctaaaaagt
    42241 aacgggttat ctaaccagta tttgccgcca gagatgacgc taacacctga acagcggcaa
    42301 ctggcgcaaa actggaatca ggggaacggg aaaaccggcc cctacgtgac cgcaattaat
    42361 ttgattcagt acaacagcca atttattggc caggacataa accaggcgct gcctggcgat
    42421 atgatttttt tcgatcaggg cgatgcccag cacttaatgg tctggatggg gcgttacgtc
    42481 atctaccaca ccggaagcgc cacgaaaact gacaacggaa tgcgcgcagt cagtctgcaa
    42541 caacttatga catggaagga cacccgatgg atacccaacg attccaatcc caatttcatt
    42601 ggcatttatc gtttaaattt tctggcgcga tagcagcgtg cttgttcctg tctcttgtgg
    42661 gaacagggct tgccaatgct gatgattcgc ttccttccag caactatgcg ccgcccgccg
    42721 ggggaacatt ctttttgctc gctgacagca gttttagcag cagtgaagag gcgaaagtgc
    42781 gactggaagc gccggggcgt gattatcgac gctatcagat ggaagagtac ggcggcgtgg
    42841 acgttcgcct gtatcgtatt cctgacccga tggcattttt gcgccagcag aaaaacctgc
    42901 atcgcattgt ggtgcaaccg caatatttgg gcgacgggct gaacaatacg ctgacctggc
    42961 tgtgggataa ctggtacggc aaatctcgcc gcgtgatgca gcgtactttt tcttctcagt
    43021 ctcggcagaa tgtgactcag gcgttacccg aattacagct cggtaatgcc attattaaac
    43081 cttcccgtta tgtacagaac aaccagtttt caccgctgaa aaaatatccc ctggtggaac
    43141 agttccgtta cccattatgg caggctaaac cggtcgagcc gcagcaaggg gtaaaactgg
    43201 aaggcgcatc cagcaatttc atctcgccgc agccgggcaa catctatatt ccgctaggca
    43261 agcaagagcc ggggctgtac ctcgtcgagg cgatggtagg tgggtatcgg gcgacgacgg
    43321 tggtgtttgt ttccgatacc gtggcgctta gcaaagtgtc aggtaacgag cttctggtgt
    43381 ggaccgcggg taaaaaacag ggggaagcga agccgggatc agagatcttg tggactgacg
    43441 gtcttggcgt gatgacccgc ggtgtgaccg atgacagcgg taccttgcag ttacaacata
    43501 tatcgccgga acgttcatac attctgggta aagatgctga aggcggcgtt tttgtctccg
    43561 agaatttttt ctacgaaagc gaaatctaca acacccgctt gtatatcttt accgaccgcc
    43621 ctctgtatcg tgcaggcgat cgtgttgatg tcaaagtgat gggccgcgag ttccacgacc
    43681 cgttgcattc atcccccatc gtcagcgccc cggcgaagct ttcagtgctg gacgccaacg
    43741 gcagtctgtt gcaaaccgtc aatgtcacgc ttgatgcgcg taatggcggg caggggagtt
    43801 tccgcctgcc agaaaatgcc gtcgccggag gttatgagtt acgcctggct taccgcaaac
    43861 aggtctatag cagcagtttt cgcgtggcaa actacatcaa gccacatttc gagattggtt
    43921 tagctctcga caaaaaagag ttcaaaacag gcgaagcggt gagcggcaaa ctgcaactgc
    43981 tttacccgga tggagagccg gtaaaagatg cccgcgtgca gttaagtttg cgcgctcagc
    44041 aattatcaat ggtcggtaac gatttgcgtt atgccggacg ttttcccgtg tcgctggaag
    44101 gcagcgaaac ggtgtccgat gatagcggtc atgtggcttt aaatctcccc gccgccgata
    44161 aaccgagccg ctatttgtta accgtctccg ccagtgacgg cgcggcgtat cgcgtcacca
    44221 ccaccaaaga gatcctcatt gaacgcggcc tggcgcatta ctctttaagt accgccgcac
    44281 aatacagtaa tagcggcgag tcggttgtgt tccgttatgc cgcgctggaa tcttcaaaac
    44341 aggttcctgt tacgtatgaa tggttgcgtc tcgaagaccg cacgagccat agcggagatc
    44401 taccgtcagg cggcaaatcc tttaccgtca atttcgataa acctggcaac tacaatctga
    44461 cattacgcga tgaagacggc ttaattctcg ccgggttaag ccatgcagtc agcggtaagg
    44521 gcagcacggc gcatacgggt acggtagata tcgtggcgga taaaacgctg taccagccag
    44581 gcgaaaccgc gaagatgctg attaccttcc cggagccaat tgatgaagca ttattgacgc
    44641 tggaacgcga tcgcgttgaa cagcagtcgc tgctatcgca tccggcaaac tggctgacgt
    44701 tacaacgttt aaacgatacc cagtatgaag cccgcatacc agtgagcaat tcctttgcgc
    44761 ctaacatcac tttttcggtg ctgtataccc gtaacggtca gtacagtttt cagaacgccg
    44821 ggatcaaagt tgccgttccc cagctggata tccgggtgaa aacggaaaaa acccattacc
    44881 agcctggtga actggtcaat gtcgaattaa cctcgtcgct gaaaggtaaa cctgtttctg
    44941 cgcagctaac ggttggtgtg gtcgatgaaa tgatctacgc gctgcaaccg gaaatcgcgc
    45001 cgaatatcgg caaatttttc tatccgctgg ggcgtaacaa tgtgcgtacc agctccagtc
    45061 tgtcgtttat cagctacgac caggcgctct ccagcgagcc ggttgcgcct ggcgcgacta
    45121 accgcagcga gcggcgagta aaaatgcttg aacgtccacg gcgtgaagag gtggataccg
    45181 cggcatggat gccgtcactc acaaccgata aacaaggcaa agcgtatttc acgttcctga
    45241 tgcctgattc gttaacccgc tggcgcatca ccgcgcgtgg gatgaacggc gacgggctgg
    45301 tcgggcaggg gcgtgcttat ctgcgttcgg aaaaaaatct ctacatgaag tggagtatgc
    45361 caacggtgta tcgcatgggc gacaaaccgg cagcaggact gtttatcttc agtcagcagg
    45421 ataacgaacc ggtggcgctg gtgactaaat ttgcaggtgc tgagatgcgc cagacgctga
    45481 cgctgcacaa aggggcgaat tatatttcgc tgacgcagaa tattcagcaa tctggcttgt
    45541 taagtgcaga actgcaacaa aatgggcaag tacaggacag cattagcaca aaactgtctt
    45601 ttgtggataa cagctggccc gttgaacagc agaaaaacgt catgctcggc ggtggcgata
    45661 acgcgctgat gttgcccgag caggcgagca atatccagct acaaagtagc gaaacgccgc
    45721 aggagatttt ccgtaacaat cttgatgcgt tagtcgatga accgtggggg ggcgtgatca
    45781 acaccggtag ccgtctgatc ccgctcagtc tcgcctggcg ttcacttgcc gatcatcaaa
    45841 gtgccgccgc taacgacatt cgtcagatga ttcaggataa ccgtctgcgg ctgatgcaac
    45901 tggcggggcc cggagcgcgc tttacctggt ggggtgaaga tggcaatggc gacgccttcc
    45961 tgacggcatg ggcatggtac gccgactggc aggccagcca ggcgctcggc gtaacgcagc
    46021 aaccggatta ctggcaacat atgctcgaca gttatgccga gcaggcagat aacatgccga
    46081 tattgcatcg cgcgctggtg ctggcgtggg cacaggagat gaatctaccg tgcaaaacgt
    46141 tgttgaaagg gttggatgaa gctatcgccc ggcgcggaac taaaactgaa gatttctctg
    46201 aggaagacac cagcgatatc aatgacagcc tgatcctcga tacaccggaa tctccactgg
    46261 cagatgcggt ggcaaacgtc ttaaccatga cgttgctgaa aaaagcgcag ttgaagtcca
    46321 cggtgatgcc acaggttcag caatatgcgt gggataaagc ggcaaacagc aatcagccgc
    46381 tggcgcacac ggttgtgctg ctcaatagcg gtggtgacgc tacccaggcg gccgctattt
    46441 taagtggttt gaccgctgag caatccacta ttgagcgcgc gctggccatg aactggctgg
    46501 cgaaatatat ggcgacaatg cctccagttg ttttgcctgc gcctgcgggc gcatgggcta
    46561 aacataagtt aactggaggg ggcgaagact ggcgttgggt tggtcagggt gtgccggaca
    46621 ttctctcttt tggtgacgaa ttatcgccgc aaaatgtgca ggtccgctgg cgtgaaccgg
    46681 caaaaacggc tcaacaaagt aacattccgg tgaccgttga acgccagttg tatcggctta
    46741 tccccggtga agaagagatg agctttactc tgcagccggt gaccagcaat gagattgaca
    46801 gcgatgcgct gtatctcgat gaaattacgc ttaccagcga gcaggatgcc gttctgcgct
    46861 acggtcaggt ggaagtaccg ctcccgccag gggctgacgt tgagaggact acgtggggca
    46921 tttcggtcaa taaaccgaac gctggaaaac agcaggggca gttgctggaa aaagcgcgaa
    46981 atgaaatggg caaactggcc tatatggtgc cggtgaaaga actgacggga acggtcactt
    47041 tccgccattt gctgcgcttc tcgcaaaaag ggcaattcgt tctgcctcct gctcgttatg
    47101 tgcgttccta tgcacctgca cagcaaagtg ttgcggcagg gagcgaatgg accgggatgc
    47161 aggtgaaata agtgaactgg cgcagaatcg tctggctatt ggcgttggtt actctgccaa
    47221 cgcttgcgga ggagacgccg ttacaactgg tgctgcgcgg tgcgcagcac gatcaactgt
    47281 atcagctgtc gtcatcaggc gtgaccaaag tctcggcatt acctgacacg ctcaccacgc
    47341 cgttagggag tttgtggaaa ctgtatgttt acgcctggct ggaggatacc catcaaccgg
    47401 agcagcccta ccagtgtcgc ggaaactccc cggaagaggt ttattgttgt caggctgggg
    47461 agagtataac gcgcgatacc gcgctggtac gttcctgtgg gctctatttt gcgccgcagc
    47521 ggttgcatat tggcgcagat gtgtgggggc aatattggca acagcgtcag gcacctgcgt
    47581 ggttagcgtc tcttactacg ctgaagccag aaacctcggt aacggttaac tcgctgcttg
    47641 attcattagc gacgttacct gcgcaaaaca aagctcagga agtcttactg gatgtggtgc
    47701 tggacgaggc gaaaatcggt gtcgcatcca tgctcggcag tcgggtacgg gtgaaaacct
    47761 ggagctggtt tgccgatgat aaacaggaaa tccgtcaggg tggttttgcc ggctggttaa
    47821 ccgatggtac accgctgtgg gtgactggca gtggcaccag caaaacggta ctgacccgtt
    47881 atgcgacggt gcttaatcgg gttttgccag tgccgacgca agttgccagt gggcaatgtg
    47941 tcctggtgga gctgtttgcc cgctatccgc tgaagaaaat cacggcggaa aagagttcga
    48001 cggcagtaaa acccggcgta cttaatgggc gctatcgtgt gacgtttgcc aatggtaacc
    48061 acattatgtt tgtcagccac ggtgagacga ccttactgag cgaaaagggc aaactgaaat
    48121 tgcagtcgca tctcgatcgt gaagagtacg ttgccagagt tctggatcgt gaggcgaaaa
    48181 gtacgccacc tgaggcagcg aaagccatga ccgtggcgat acgcactttt ttacagcaaa
    48241 acgccaatcg ggaaggcgat tgcctgacca tccctgacag cagcgccacg cagcgtgttt
    48301 ctgcttcgcc agctaccacg ggggcgcgaa cgatgaccgc ctggacgcag gatcttatct
    48361 atgcgggcga tccggttcat tatcacggca gtcgcgctac cgaagggacg ctttcctggc
    48421 ggcaagccac ggcccaggcg gggcagggag agcgttacga tcagatactc gcgtttgctt
    48481 atcccgacaa tagccttagc cgctggggag cgccacgctc aacctgccag ttattaccca
    48541 aagcgaaagc ctggctggcg aaaaaaatgc cgcagtggcg gcgtatatta caaggtgaga
    48601 cggggtacaa cgaaccagac gtgtttgcgg tttgccgttt agtctcaggt ttcccctata
    48661 ccgatcggca gcagaaacgg ctgtttattc gtaacttctt tacgtttcag gatcggctgg
    48721 atttaaccca tgagtatctg cacctggcct ttgatggtta tcccaccggg cttgatgaga
    48781 actatatcga aacgcttacc cgacaattat taatggacta atgctatgcg aaagattttt
    48841 cttccgttgt tactggtggc gctttcgcct gtcgctcaca gtgaaggtgt gcaggaagtc
    48901 gagattgatg caccgctctc tggctggcat ccggcagagg gtgaagatgc cagtttttca
    48961 caatcgatca attacccggc gtcgtccgtc aacatggcag atgatcaaaa tatctcagcg
    49021 cagatccgcg gcaaaataaa gaattatgct gcggcgggta aagttcagca gggccggctg
    49081 gtggtcaacg gtgccagtat gccgcagaga attgaatccg atggttcatt tgcacgccct
    49141 tatattttca cggaaggcag caacagcgtg caggtcatca gcccggatgg gcaaagccgg
    49201 caaaaaatgc agttttactc aacgccgggt acaggaacga ttcgtgcacg tttacggctg
    49261 gttctctcgt gggatacgga caataccgat ctcgatctcc atgttgtcac gcccgatggc
    49321 gaacacgcct ggtacggtaa cacggtgctg aaaaacagtg gtgcactgga tatggatgtc
    49381 acgacggggt acggacccga gattttcgcc atgccagcgc cgattcacgg acgttatcag
    49441 gtgtatatca actactatgg cggacgcagc gaaacggaat tgacgaccgc ccaactgacg
    49501 ctgatcaccg atgaaggatc ggtcaatgag aaacaggaaa catttattgt accgatgcgt
    49561 aatgctggcg agctgacgct ggtgaaaagt tttgactggt gattaatggg gttaccttcg
    49621 ccgtcgatgt tcaagaaaac acccgataac tttcgctatc gggtgttttt attgattaat
    49681 tcaaccgttc aatcaccatc gcaattccct ggccgccacc aatgcatagt gttgccagcc
    49741 ccagcgtttt atcgcgtgcc tgcatggcat gtaatagtgt gaccagaata cgagcaccac
    49801 tggcaccgat aggatgcccg agcgcgatgg ccccgccgtt gacattcact ttctcagaat
    49861 caaagcccag ggttttccca acggcaagga actgtgcagc aaatgcttca ttagcctcaa
    49921 tgagatcaat atccgccagt tgcagccccg ccagttgtaa cgcttttttc gtggcaggta
    49981 ctggccccat acccatcaat gcggggggca cgccaccgct ggcataactt ttaatgcgag
    50041 ccaggggggt aaggcctgct gccagcgccg cagattcttc cataatcacc agagcggcag
    50101 caccgtcgtt aataccagac gcgttcccgg cggtgactgt tcctgcttta tcgaaggccg
    50161 ggcgcaatgc gcctaacgct tcagtcgtag aatccgcttt cgggaattcg tcttgactga
    50221 agacgaaggt tttcttccga gtgacaacat ttaccgggac gatttcggct gtaaaagcac
    50281 cggactcaat tgcggctgcc gctttacgct gtgaatgtag cgccagttca tcctgcattt
    50341 cacgggtaat tccgtactct ttagccacgt tttcggcggt aatccccata tgataaccat
    50401 gggtggcgca catcagacca tcgcgcagga ttacgtcata aacctgtccg tctccaagac
    50461 gataaccaga gcgtgctttt gcatcgagta agtagggcgc taaactcata ttttccatac
    50521 cccccgccac aatgctctgc gcctgacctg cctgaattgc ctgggcggca agcgccacac
    50581 ttttaagacc cgaaccacac actttattga ccgtgaatcc gcacaccgtt tcagccagtc
    50641 cgctttttag cagcgcctga cgtgccggat tttgccccag tccggcttgc aacacgttac
    50701 ccataatcac ttcatcaacg tgtagtgaat cgatttttgc acgttcaatg gcggctttaa
    50761 ttactgtcgc ccccaggtcg atggcgctgg tggaagcgag tgaaccgtta aaactaccga
    50821 tagcagtacg taccgcactg acgatgacac aatttttcat tttatattcc ttctgtctgt
    50881 agggttgccg tcagaacagc gttaaaccaa tgacgaaaat gacgccggag aagagcaagg
    50941 cagtgatgca atagcccatg atgtcgcgga caccgagtcc ggcgattgcc agtgctggca
    51001 gcgcccagaa cggttgtgcc atgttcatcc attgctcgcc gtaggcgatc gccattaccg
    51061 atttaccgag atcagcgccc agcgcctggg ctgcgggcat cacgaacggc ccctgaataa
    51121 cccagtgccc gccgccagac ggaacggcga agttaatcag tgcagaacta aaaaaggtca
    51181 tgatcgggaa ggtgtctttg ttcgcaacat tgatgaagaa ttcggtaatg agtccgccca
    51241 gaccggaatg ctccatcatc agttggatcc cagcgtagaa ggggaattgc accagaatac
    51301 cggcagtact gcgtgctgcc gcgctgatag cacgcatata agccattggc gttttatgta
    51361 gcagcagacc cgcaatcata aacatcaggt tgacggtatt gatggtgatg ttgaagccat
    51421 gttcgctgaa gtacatcgca aggtaagcga taccgagtgc gccgatgatc aacgtcagaa
    51481 tgcggctttc ttccagtcgc tcggatggtg gggcatcttt cggtagctgc ttttgaaaat
    51541 cagcctcttc catgagtagt ttcggatcga tactcaccac gtcagacggt tttggcatca
    51601 tcatgcgggt gataaatggc atcaccacaa tcaacgccac agtgatgaaa atattaaaac
    51661 cactgaacag agtatcgccc accgggatca gcccggcgat atgctcaacc gggttgcccg
    51721 gtgttgcagc caacagaggc attgagccag agaaaccgcc accccaggtg agaaaaccaa
    51781 tgtaggcgca ggcaatgagc aacggataat cagaaccggg tactcgccgg gcgacttcac
    51841 gggcgaacat tgcaccgaca accaaaccaa atccccagtt gatgacacaa gcgactgaac
    51901 cgaagaaagt aaccagcatg acgccctgta cgggcgtctt tgcggcggag gcggcagtac
    51961 gcagcaaact tttcaccgga gcggagctgg caagggcatg accggtaacg atgataagcg
    52021 ccatctgcat accaaacgcc agcaagttcc agaaaccatc accccacatt ttcaccatgc
    52081 tgatcggcgt ttgtggtgtt aaccaaagcg cgatcacgaa tgttagcaat gtcagcaaca
    52141 tggcaaagat cagtggatcg ggaagccacc ggctgacaaa acgcgtcata aaacgcgata
    52201 tgcgaccaat cataaatcac cccgttgcgt attcagatcg gcggcaactt caaaccgagc
    52261 ttctgttttg gcacgcacgg tggctaaatc acacccgtcg gcaatttcgg tgagccacat
    52321 tttgccgtca ataaaacgaa agacagccag ttcagtaacc agcatatgca ccgcatgttg
    52381 cgcagtgagt ggcatggtgc agcggcgcaa aatttttgct gaaccatctt tggcgcaatg
    52441 ttccatggcg atgatcactt tgcgtgaccc ggtcaccaga tccatcgcgc cacccatacc
    52501 gggcaccatt ttcccaggca ctacccagtt cgcgaggttt gcttcttcgt ctacttgcaa
    52561 accgccgagc acgcaggcat caatatgacc gccgcggatt agcgcaaatg acatggcgct
    52621 atcaaacatg gctgcgccgg gtaaaatgcc gcacggttgt ccgccagcgt tcaccagatc
    52681 tggatgcgct gtcgtgaccg ggcctaaacc gaggaagccg ttttccgatt gcagagtgat
    52741 atgaataccc tccggtaaat aattggcgac cattgtgggt aaaccgatcc ctaagttaac
    52801 gatgtcacca tcacgaagct cttgcgccac acggcgcgca atacgttgtt tcgcatccat
    52861 tatttgctct cctgtgaaac gatgatgtgg tcgataacgg caccaggggt gacaatatgg
    52921 tcaggttgca gctcgccggt ttcgaccagt tcatctggct ctaccagcgt gatatcagcc
    52981 gcaagggcta tcagggggtt aaagttgcgg gcgctaagtt gataggtaag gttgccaagt
    53041 gtgtcgcaac gatgagcgcg aattagcgcc aggtcggcgc gcagcgggcg ttcgagcagc
    53101 caggttttac cgtcgagtgt cagtgtctgt ttgccttcct ctacgacggt gccgacacca
    53161 gttggggtga gaaaaccacc aagtccagct ccgccacagc gaatttgctc gattagcgta
    53221 ccttgcggca ccagaacgac gtccatctca ccagatatca tgcgccgacc tgtttccggg
    53281 ttggtgccga tatgtgaagc aatcactttg cggactcgac cattgacgat gagcggaccg
    53341 atgccggtat caacaaacgc ggtatcattg gctatcaatg tcaggtcgcg aacaccagat
    53401 tccagtaatg cttcaaccag gcgggatgga gtgccaatcc ccataaatcc gcccaccatg
    53461 atggtcatgc cgtcacgaaa gaagccggtg gcgtcttgta atgtcatcaa ttttgttttc
    53521 attttttatc cctcttgcat acaccgttgt gggtacagtc agggaatagc aaggggagtg
    53581 ccagaatgag attattgaat gttgttattt aactaattga atagaaagga ataaattaga
    53641 tggggattca taaggagaat gagcggctat gcagaaaatt gcgcactgtg caaatttctg
    53701 catagcaagt tttggcgtta tacatccgcc ggatcgatac cgtattcctg gagtttatac
    53761 atcaatgcac ggcgactgat gcccagcatt aacgcagtgc gggttcggtt tccttcttgt
    53821 tgttccagca cttccataat gatgcgtttt tcgacgcgtt taatttcctc ttttaaatta
    53881 cgctcaccga caggggctgt ttttgcctca ccagcattac agactggctg acgaatctgc
    53941 ggcggaagat cctcagagaa aatgatcggg cctgagttca tcacgacggc gcgttcaatg
    54001 acgttggaaa gctctcgaat attacccggc catgaccagg cggtaagcag cgacattgcc
    54061 atcggatcga tgtcgataat atcgcgctga ttctcactac tgaatttttg caaaaagtga
    54121 ttagctaaca gggaaatatc ttcccggcga tcgcgcagag gcggcagtat taaatgaata
    54181 acgttaaggc gataaaagag atcttcacgg aaggtgcctt cttttaccat tgcctgcaag
    54241 tcacggttgg tggcagcaat gatgcggata tcaactttta tggtctgatg accgccaatc
    54301 cgttcaaatt cccgttcctg cagaatgcgt agtaatttgg cttgcagtac gagcggcatt
    54361 tcgccaattt cgtcgaggag cagagtacct tcgttggcac gttcaaataa tccctgacgc
    54421 aaggtttgtg caccagtaaa tgcacctttt tcatgaccaa acagttcact ttcgagcaac
    54481 gattccggca gcgccgcgca gttgacttta atgaacggcc cctttgcccg ccgcgaattg
    54541 tagtgaatcg ctctggcaat caactctttc ccggtgccgc tttcaccgct aatcaagacg
    54601 ctggcctgag aaagggcaat tttggcggtg tctttgcaga tatccatcat cgccgggctg
    54661 ttggtgagaa tgtgccccca ttgccagctg gtgctcagtg cctggtgcag atgacggatc
    54721 tcttttttca ttgactggag ttgtaaagcg cgctgaacga ttaaattcaa ctcatcgaga
    54781 tcaaacggtt taataacata gtcgaaggcc ccgcagcgta gcgcttcgac ggcggtttcc
    54841 acttcagcat aggccgtcat cagaataacg ggtgtccggg tctcattact gcgcatctcc
    54901 tttagtgcct tgatgccgtc catctctggc atgcggatat ccatcaacac cacgtcaggg
    54961 tgaatatcag caaacaggtg taatgctgtg cgtccgttgt tcgcacaatg tgtttcgaaa
    55021 ccttgtagtg caaaagcggt gctcagcata cggcgaacat tatcttcatc atccacaata
    55081 aggatgcgat taatagcagt catacagtct gatttccctg cgggttgatc ggtaaaataa
    55141 gcgtgaaggt tgcgccgtag cccggcaaac tggcgacgcg aatatcaccc tgatgggcat
    55201 taatgatgcg ttgacttaac gccagaccaa gcccggttcc tgaggctttg gtggtgaaaa
    55261 agggatcgaa gatctttttt tgcagcgaga gatcaatgcc acagccgttg tcctctatcg
    55321 aaatggcctg ttgtgagtcg ctgtattgcc aggttcgaat gcgaattttc cctcgtgcac
    55381 tgatagcctg gacggcattg atcaggatat tcagtagtac ctgtttgagc agttcacgat
    55441 cggcgttaat cgggcttaat tcattatcca gttcgcttat gaagtcgacc cgcgcttgta
    55501 cgccggcggt ttgtaccagt accagagttt cttcaaccaa tgcattgagg ctgacttgtt
    55561 gccattgact gtggcgtggg cgtgaaaatt cgagcaattg ctgaataact ttgttaattg
    55621 aatcgatttc tttgagtact acggacagat attcctgatg tattgggtca cgggtttgtt
    55681 ggcgcaagat ctgtacataa ccacgaatag ctgttagcgg attacgtact tcatgcgcca
    55741 cgccagccat cagctcaccc agtgtggcga ggcgttctgc ttgcgccatg cggcgctggg
    55801 tttctttgcg ggcagttaaa tcagagaaaa tcaccaaagc acctatcatt tcaccgtgcg
    55861 tgttatgaat acgactggtc gtgacactga gttcaatggt gcggtcacgg cctggaaaac
    55921 tgatctccag tgctacatgt tcggtgccat gttccagcgt atccagtacg ggactgtaga
    55981 actgagtatt gtcgaacaac atggagtaag gctgccctac cagttcatga cgttgatagc
    56041 cagtgatgac ttctgctgct gggttcatgg tggttacatc accctggcgg tcaatggcaa
    56101 tgacgccatc ggcagcgttt tcaataatca gatcgttaag tgttcgcgtt tcacgcagtg
    56161 cctgggcgag gttgttaaca ctctgactga tttgccccat ttcaccgggc aattgtggta
    56221 atcgagtggg aatattttga gccagagtcg agaggccatc ggtgatgata tctatattgg
    56281 cgctcaggcg acgggagaaa aggacaatca acagcaggct tatcagcaaa ccggcggtga
    56341 gcacaatgat aatcctcaca tccattttcc aggcctggcg gcgaatatct tcggttaatt
    56401 cattggccca gatatagccg aggatttcac cattacgctc aatgggaatc attgaattca
    56461 aaatatcgcc gcgcacctgt ctgcctgaat aaaccaaagg ggtatttgta cgcatgactt
    56521 cgcgaccagg gtgatctgcg gcaatggtaa cgcccacatt attctgatat agcgctgaag
    56581 gcgcgtaggt gattatcgca tccagcgttt tgttgtaata accagcgccg atgccaggga
    56641 aggcgtgagt gatattttcg gtaatggggg caagttctgc atttaatgcg cggatacgct
    56701 cctcacgcgg taagtcgatg tagagatcgt agcgattgcc tagcgcctga ttaagcaggt
    56761 tgaccacggc agaaagtttt ttctcttttt cagataagac tgctgaacgt ccttccgttt
    56821 ctacgatata accaatagta agcgttggga caatgaccat caggattgcc atcaggatca
    56881 tttgattacg taagcggcgt ggataaatcc acttcatata atgcatgctg aaatccacta
    56941 gtcttgtccg gtatatgacg attatcagag gttaaggtga tgatttctcg gcggtgtatc
    57001 atattccaga gaagagagaa cattgcggta acacgctttt accgctacct taaccacact
    57061 ccatcggtca cctgaggcgg agcttcgccc ctttgaaata ccttgcttct tttcgtacaa
    57121 ccctgaaagc ctcgcgctac atgttcagag cattggcgtt agtgctctgg ctgttgattg
    57181 ctttttcatc cgttttttac atcgttaatg cgttacatca gcgagaatcg gaaattcgtc
    57241 aggaatttaa tctgagttcc gatcaggctc agcgctttat tcaacgcacc tctgatgtga
    57301 tgaaagagct gaagtacata gctgaaaatc gcttatcggc agaaaacggt gtgctttccc
    57361 cgcgtggacg agaaacgcag acggatgtgc ctgcgtttga accgctattt gctgactctg
    57421 attgttccgc aatgagtaac acctggcgag gttctctgga gtcattggcg tggtttatgc
    57481 gctactggcg cgataatttt tctgcggctt acgatctcaa ccgggtattt ttaatcggca
    57541 gcgataacct ctgcatggcc aatttcggtc tgcgtgatat gccagtggaa cgcgataccg
    57601 cgttaaaagc tttgcatgaa cgcattaata aatatcgaaa tgcaccacaa gatgatagtg
    57661 gcagtaacct ctactggatc agcgaaggtc cgcgccctgg cgtcgggtat ttttacgcct
    57721 tgacgccagt ttatctggcg aaccggttgc aggcgctttt gggtgtcgag cagaccatcc
    57781 ggatggagaa ctttttctta cctggtacgt tgccgatggg ggttaccatt cttgatgaaa
    57841 atggtcatac cctgatttcg cttaccggac cagaaagcaa aattaagggc gatcctcgct
    57901 ggatgcagga acgctcctgg tttggctata cggaagggtt ccgggagctg gtgctgaaga
    57961 aaaatctgcc accctcatcg ctaagcatcg tgtattcggt gccggttgat aaggtgctgg
    58021 aacgcattcg catgttgatc cttaacgcaa ttttgctgaa tgtgcttgcc ggagctgcat
    58081 tgtttactct cgcgcggatg tacgagcggc gtattttcat tccggcggaa agcgacgccc
    58141 tgcgactgga agaacatgag cagtttaatc gcaagattgt cgcctccgcg ccagtgggta
    58201 tctgcatttt gcgtaccgct gatggcgtca atattttaag taacgaactg gcgcatacct
    58261 atctcaatat gcttacgcat gaggaccgcc aacggctgac acaaattatc tgtgggcagc
    58321 aggtcaattt tgttgatgtc ctgaccagca acaataccaa cctgcaaatc agcttcgtcc
    58381 attcgcgcta tcgtaatgaa aacgtggcca tttgtgtgct ggtggatgtt tcttcgcgcg
    58441 tgaagatgga agagtcgttg caggagatgg cacaagcagc ggaacaggcg agccagtcaa
    58501 aatcgatgtt ccttgccacc gtcagtcatg agctgcgaac gccgctgtat ggcattatcg
    58561 gtaacctgga tctgttgcaa accaaagagt taccgaaagg cgtcgatcgt ctggtgacgg
    58621 caatgaacaa ctcttccagc ctgttgttga aaattatcag cgatattctc gacttctcga
    58681 agattgaatc ggaacagttg aagatcgaac cgcgtgagtt ttcaccgcgt gaagtgatga
    58741 accacatcac cgccaactat ttaccgctgg tggtacgcaa gcagctaggc ttgtactgct
    58801 ttattgaacc ggatgtgcca gtggccttaa atggcgaccc gatgcgttta cagcaggtca
    58861 tctccaacct gttgagtaac gccataaaat tcaccgatac cggctgtata gttctgcatg
    58921 ttcgcgctga tggcgattat ctctctatcc gtgttcgcga taccggcgta gggattccgg
    58981 cgaaagaagt ggtgcgcttg tttgatccct tcttccaggt cggaacgggc gtacagcgta
    59041 acttccaggg gaccggtctg ggtctggcga tttgtgaaaa actgatcagc atgatggacg
    59101 gcgacatctc ggtagattca gaaccgggaa tgggcagcca gtttaccgtg cgtattccgt
    59161 tgtacggcgc tcagtacccg cagaaaaaag gcgtggaagg gttgagtggt aaacgttgct
    59221 ggctggcggt ccgcaatgcg tcgctctgtc agttcctgga aaccagtttg cagcgcagcg
    59281 gcatcgtcgt tacaacatac gaagggcagg aaccgactcc cgaagatgtg ttaatcactg
    59341 acgaggtagt gagcaaaaaa tggcagggta gagcggttgt gaccttctgt cgtcgccata
    59401 ttggtattcc gctggagaaa gcgccaggag agtgggtaca cagtgtggct gccccgcatg
    59461 agctaccggc attgttggcg cgtatttatt tgatcgagat ggagagcgac gatcctgcta
    59521 acgctctgcc gttaacggac aaagcggtca gcgataatga cgatatgatg attctggtcg
    59581 tggatgatca tccgattaac cggcgtctgc tggcagatca gttgggatcg ttgggctatc
    59641 aatgtaaaac cgcgaatgat ggcgtcgatg cgcttaatgt acttagcaag aatcatattg
    59701 atatcgtgct tagcgacgtc aacatgccaa atatggatgg ttaccgcttg acgcaacgca
    59761 ttcgtcagtt gggactgacg ttgccggtaa tcggagtaac tgctaatgcg ttggctgaag
    59821 agaagcagcg gtgtctggag tccggtatgg acagctgcct gtcgaagccg gtaacgctgg
    59881 atgtgataaa acagacgctg acggtatatg ccgagagggt caggaaatcg cgggaatcgt
    59941 aggacggata aggcgtttac gccgcatccg gcaatccgtg caaatgccag ataagacact
    60001 gacgcgtctt atctggccta caggtgatta gtctttatct gccggactta acgtcacaga
    60061 agagagataa ttcagcagag caatatcgtt ctcgacaccc agcttcatca tcgcagattt
    60121 cttctggcta ctgatggttt tgatactgcg gttcagcttt ttagcgatct cggtcaccag
    60181 gaaaccttcc gcaaacaggc gtagaacttc actctctttt ggcgagagac gcttgtcacc
    60241 gtaaccacca gcactgattt tttccaacag gcgagaaacg ctttccgggg taaatttctt
    60301 ccctttctgc agcgcggcga gagctttcgg cagatcggtc ggtgcacctt gtttcagcac
    60361 gatcccttcg atatccagat ccaataccgc actaagaatc gccgggttgt tgttcatagt
    60421 cagaacaatg atcgacaggc ttgggaaatg gcgcttgatg tacttgatta aggtaatgcc
    60481 atcgccgtac ttatcgccag gcatggagag atcggtaatc aacacatgcg catccagttt
    60541 cggcaggttg ttgatcagtg ctgtagagtc ttcaaattcg ccgacaacat tcacccactc
    60601 aatttgctca agtgatttgc gaataccgaa caagactatc ggatggtcat cggcaataat
    60661 tacgttcata ttgttcatgt acttgcacct atgtattaac tatttgattt taaaaatata
    60721 ttctatgctt tgtttagtcg ttggggcacc tgtgggacat aagtacatag ctttctgttt
    60781 agtacttcta cctgggactg gttattgtct ttcatccatg caccgtaaac attgtaaacc
    60841 atctgggcat tcgcatgtcc catctggttg gctatgaagt ttggatttgc tccggcagtt
    60901 aaagcccagc aagcataagt atgccgagac tgatacgctt ttctatggcg aatttttgct
    60961 cgttttaatg cagattccca tgttctgttg atggatgtta cggcataatg aactcctgag
    61021 ttacctgtgc gatctaatat ctgaggattg aaaacaaagc tgcatgagtg agtgcttgtc
    61081 cggccatact ctcgcagctt tacttctatc tgatattgtt taccaagcct ggttaatgtc
    61141 gcctgatttt ttaatacgtc aattgctggc tgtatgagtt gtattaccct gtttgttcct
    61201 gcatctgttt tgggtagtgt aaattccttt gtctgagtgt aattcctttt gacggtcaag
    61261 gtacccgctt ccagatcgat atcctcccat gccagcccac acagttcgcc atgccgcatc
    61321 ccggtatata cagccagcga ccagaaattt ttagtttgct ggtggtggca cgcatcaatc
    61381 agtctggcaa actcatcttt tgttagtgga tcgggaggtg ttttcgctct tttcagtgat
    61441 ggaagctcac tgaaagggtt tttgcctata tacccattgt ttgccgcaaa acggaacatc
    61501 ccggagataa ttgtcatata gctatttaca gtaggtacac tacgaccttt aataggcttc
    61561 ctctgatttt ttcctggcaa ctgatatccc gttaataatt ctttacgaaa aatgagaaca
    61621 tcttcctgtg taattgatga tgccagtttg ttccctccaa ggcagtcaag gcaatttcgg
    61681 atagctgatt tgtatctgac catggcattt gctgttattt ccattttctt aagctcaagc
    61741 catttttccg aaagtgcctt aatggttatc tctttttttc ccagaccaaa atgtttcagg
    61801 ttaggggaat tagggaactg cgcggcgtag tcgaagctcc ccattcttat tgcaaaacaa
    61861 acggaagtgc gaagttcacc tgcgatcttc cggtttttgg cggtgtcagg aacaccgagg
    61921 ttttctctga cacgtttgcc gttatagtga aaccatatgc ggagtgatcc gccatggttt
    61981 tcaacgcctg tcgggtatga tgcgttactc attaaacctc ccagacgtcc aggagcatta
    62041 acaggttaac cggaacttgc atttttggca cctggttgtt tctggttttc gatccagcgc
    62101 ataatttcct caatgttgta tacacattca ctgaaaggcc cgggctctcc ttctgctgcg
    62161 taatgacggt attctttccc cagtaaccag gatttttttc ttgctctggc aatggttcct
    62221 gatttcagtc cagttgcagc aataagggtt ctttctgtgc accatttgct gggcgttatc
    62281 tgatagatga ttgtttccat tcctgactca cactatcttc aggccacggc agtggcacca
    62341 cacgtcaaac attcgcttta caacttcacg gcagtagaag ccgtgagcat ctctggttaa
    62401 atcgtatcgg tacccgtatc tgagtcgtat ccatatttca aactcgcgat tcacgactta
    62461 ttctcctgct gtctttatgc cagttcattc ggttgcgctg gggccctggc cttccgtggc
    62521 ctgtatttgc ggcgttttct ggcttcaatg cgtatctttg tcaccctgac tggctttggt
    62581 tcgtgccgga acactttgtt tctgatatta cgccgtgtat tcatcatcca gataaaacgc
    62641 tgggtatggt cgcatccgtc atcaacggta attactgtgc ggaccagttc atcgttaata
    62701 tcaaccattt cacccattca ctgagccttc atacgcgaat ttcgtggctt ccatcagttg
    62761 cgtccatacc cattcaagaa attgtgcgtt ttctcctgag cggtcatatt catcgacgct
    62821 gcggggcttg atggtcgcca gtagtggcac aatccggtac ttcatctgtt cggttaactt
    62881 gtccggtaca agtcgccagc cgttatattt cgatggaaca tggtggtatt cagccaggaa
    62941 gcgtcgtata tcgtttttca tttcctcgca tgagttctga tcatgaagtg cagcggaaag
    63001 ccagcgtccg atatattcag ggattcgcac atcaagtact ttttctgccg tcgtcagcat
    63061 tgcctgtcgg caggcgttcc agccgttgac atagtgacgt gttgcatccc atgaaacctg
    63121 cacatcaggg taagcatcct gttctgttct cactggcggt atctgaggaa cagtgaatag
    63181 tggactgacg atatatttat ccagagagcc tccccgctct tctgcaagac tttcccacga
    63241 tgtcattgtg gggaccctgt aacctgcttt tttgtgcgta tacatccatg ccactctgcc
    63301 gttatcatct gcggcggcaa tgcgctgcca ggcaggataa agcggggtgt atgtttctct
    63361 gtcattacgg gcgtcaggca tggcatcaat actgcaaact tcccccgtaa ggttattcag
    63421 ccaggccttc gggctaatct ctgacgttac gatttcatcc agttttttct ccagattttc
    63481 tatgtgactg attagcgcaa ggataactgg cggagtggca agggagtgaa atgc
//
LOCUS       DAZGQL010000015        62789 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.015, whole genome shotgun sequence.
ACCESSION   DAZGQL010000015 DAZGQL010000000
VERSION     DAZGQL010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 62789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62789
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.015"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..132
                     /locus_tag="ACHKIR_001130"
     CDS             <1..132
                     /locus_tag="ACHKIR_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HFO9988897.1"
                     /translation="VLATLTACTGHIENRDKNCSYDYLLHPAISISKIIGGCGPTAQ"
     gene            complement(239..1324)
                     /gene="yhfS"
                     /locus_tag="ACHKIR_001131"
     CDS             complement(239..1324)
                     /gene="yhfS"
                     /locus_tag="ACHKIR_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFO9988898.1"
                     /translation="MKTFPLQSLTLIEAQQKQFALVDTICRHFPGSEFLTCGDLGLTP
                     GLNQPRITQRVEQVLADAFHAPAAALVQGAGTGAIRAALAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITADFNDLSALKQVVDEQQPDAALVQHTRQQPQDRYVLADVLATL
                     RAAGVPALTDDNYAVMKVSRIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRA
                     TLYSGGSQIQGAQALEVLRGLVLAPVMHAVQAGVSERLLALLNGGAVAEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLE
                     HYAIRINPNRSGEETVLRILRESIASI"
     gene            complement(1336..2640)
                     /gene="yhfT"
                     /locus_tag="ACHKIR_001132"
     CDS             complement(1336..2640)
                     /gene="yhfT"
                     /locus_tag="ACHKIR_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HFO9988899.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLI
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(2652..3005)
                     /gene="yhfU"
                     /locus_tag="ACHKIR_001133"
     CDS             complement(2652..3005)
                     /gene="yhfU"
                     /locus_tag="ACHKIR_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HFO9988900.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGCFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(3016..3894)
                     /gene="php"
                     /locus_tag="ACHKIR_001134"
     CDS             complement(3016..3894)
                     /gene="php"
                     /locus_tag="ACHKIR_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HFO9988901.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     AQGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVATRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNIVKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRETPSQFFQ"
     gene            complement(3891..5117)
                     /gene="yhfW"
                     /locus_tag="ACHKIR_001135"
     CDS             complement(3891..5117)
                     /gene="yhfW"
                     /locus_tag="ACHKIR_001135"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFO9988902.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPMLEKMGLINALGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALLNADWQVERRGDDLQFLWVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVREQVQVGRVITFGGLLTDSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEVGVPTVLVGKVADIASNPYGVSWQNLVDSQRIMD
                     ITFNEFNTHPTAFICTNIQETDLAGHAEDVARYAERLQVVDRNLARLIEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGMVATQLGVLTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(5117..6280)
                     /gene="yhfX"
                     /locus_tag="ACHKIR_001136"
     CDS             complement(5117..6280)
                     /gene="yhfX"
                     /locus_tag="ACHKIR_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HFO9988903.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQILENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPLAHQ
                     GHLVQIPCHQVSDAVAQGTDVITVFTLDKAREVSAAAVKAGRVQSVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVVADIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLIQAR
                     DQLAKSGIAIEQLNAPSATSCTSLPLLAEYGVTHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEFPVSSAVVLCFRTQIFVTRSDVVLLSGIHRGEPEIVGRYDSLGNSLGA"
     gene            complement(6364..6726)
                     /gene="yhfY"
                     /locus_tag="ACHKIR_001137"
     CDS             complement(6364..6726)
                     /gene="yhfY"
                     /locus_tag="ACHKIR_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFO9988904.1"
                     /translation="METRLNLLCEAGVIDKDICKGMMQVVNVLETECHLPVRSEQGMM
                     AMTHMASALMRSRRGEEIEPLDDELLAELAQSSHWQAVVQLHQVLLKEFALEVKPCEE
                     GYLLANLYGLWMAANEEV"
     gene            complement(6743..7648)
                     /gene="yhfZ"
                     /locus_tag="ACHKIR_001138"
     CDS             complement(6743..7648)
                     /gene="yhfZ"
                     /locus_tag="ACHKIR_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator YhfZ"
                     /protein_id="HFO9988905.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGLCLALE
                     LGPHTYVGEHQLICRKGESANVNRVGLDNRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKQALLAHQQRVVSGEQEPSY"
     gene            complement(7938..8942)
                     /gene="trpS"
                     /locus_tag="ACHKIR_001139"
     CDS             complement(7938..8942)
                     /gene="trpS"
                     /locus_tag="ACHKIR_001139"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFO9988906.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASAHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(8935..9693)
                     /gene="gph"
                     /locus_tag="ACHKIR_001140"
     CDS             complement(8935..9693)
                     /gene="gph"
                     /locus_tag="ACHKIR_001140"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HFO9988907.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADILMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(9686..10363)
                     /gene="rpe"
                     /locus_tag="ACHKIR_001141"
     CDS             complement(9686..10363)
                     /gene="rpe"
                     /locus_tag="ACHKIR_001141"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFO9988908.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(10381..11217)
                     /gene="dam"
                     /locus_tag="ACHKIR_001142"
     CDS             complement(10381..11217)
                     /gene="dam"
                     /locus_tag="ACHKIR_001142"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFO9988909.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVD
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(11324..12610)
                     /gene="damX"
                     /locus_tag="ACHKIR_001143"
     CDS             complement(11324..12610)
                     /gene="damX"
                     /locus_tag="ACHKIR_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HFO9988910.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPELEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPATTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(12702..13790)
                     /gene="aroB"
                     /locus_tag="ACHKIR_001144"
     CDS             complement(12702..13790)
                     /gene="aroB"
                     /locus_tag="ACHKIR_001144"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFO9988911.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(13847..14368)
                     /gene="aroK"
                     /locus_tag="ACHKIR_001145"
     CDS             complement(13847..14368)
                     /gene="aroK"
                     /locus_tag="ACHKIR_001145"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HFO9988912.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(14769..16007)
                     /gene="hofQ"
                     /locus_tag="ACHKIR_001146"
     CDS             complement(14769..16007)
                     /gene="hofQ"
                     /locus_tag="ACHKIR_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HFO9988913.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSVAWQNDNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQQAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(15919..16323)
                     /gene="hofP"
                     /locus_tag="ACHKIR_001147"
     CDS             complement(15919..16323)
                     /gene="hofP"
                     /locus_tag="ACHKIR_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HFO9988914.1"
                     /translation="MRVKRWLLAGITLCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDALTLGTGTNCEPPHWLWQRQ
                     GDTNEAMDSRTTVDAYTRRTGGKAAKSDADGG"
     gene            complement(16313..16753)
                     /gene="hofO"
                     /locus_tag="ACHKIR_001148"
     CDS             complement(16313..16753)
                     /gene="hofO"
                     /locus_tag="ACHKIR_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HFO9988915.1"
                     /translation="MDMLFDWWFATSPRLRQLCWAVWLLMLVTLIFLSSTHHEESDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVYWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            complement(16737..17276)
                     /gene="hofN"
                     /locus_tag="ACHKIR_001149"
     CDS             complement(16737..17276)
                     /gene="hofN"
                     /locus_tag="ACHKIR_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HFO9988916.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTS
                     NAEARVDTVLLQAEQQLARSLQITKPRLLERQQLREQRLQRQLQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDGHAL"
     gene            complement(17276..18045)
                     /gene="hofM"
                     /locus_tag="ACHKIR_001150"
                     /pseudo
     CDS             complement(17276..18045)
                     /gene="hofM"
                     /locus_tag="ACHKIR_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
     gene            18165..20717
                     /gene="mrcA"
                     /locus_tag="ACHKIR_001151"
     CDS             18165..20717
                     /gene="mrcA"
                     /locus_tag="ACHKIR_001151"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HFO9988917.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQEATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRHDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(20885..21445)
                     /gene="nudE"
                     /locus_tag="ACHKIR_001152"
     CDS             complement(20885..21445)
                     /gene="nudE"
                     /locus_tag="ACHKIR_001152"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HFO9988918.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVFEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRL"
     gene            21765..23900
                     /gene="igaA"
                     /locus_tag="ACHKIR_001153"
     CDS             21765..23900
                     /gene="igaA"
                     /locus_tag="ACHKIR_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HFO9988919.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAHDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMKIQAYYESCLNPQLITPSESLIE"
     gene            23965..24633
                     /gene="yrfG"
                     /locus_tag="ACHKIR_001154"
     CDS             23965..24633
                     /gene="yrfG"
                     /locus_tag="ACHKIR_001154"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HFO9988920.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            24644..25045
                     /gene="hslR"
                     /locus_tag="ACHKIR_001155"
     CDS             24644..25045
                     /gene="hslR"
                     /locus_tag="ACHKIR_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HFO9988921.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMALAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            25070..25948
                     /gene="hslO"
                     /locus_tag="ACHKIR_001156"
     CDS             25070..25948
                     /gene="hslO"
                     /locus_tag="ACHKIR_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFO9988922.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(26086..27810)
                     /gene="yhgE"
                     /locus_tag="ACHKIR_001157"
     CDS             complement(26086..27810)
                     /gene="yhgE"
                     /locus_tag="ACHKIR_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HFO9988923.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATLGMSGWLKWQIDGMNPWRAEKALWD
                     FGCYLLLMAMLLLPWIQQSLRIRNDSSRYRYFYQSVWHNVLILLVIFLANGLTWLVLL
                     LWGELFKLVGITFFKTLFFATDWFIYLTLGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVAAWALWLRVAQYGWTVDRLQGALAVLVLLVWSLGY
                     FVSIVWRKGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVSLYMLEQSGRYGRAALESLKSDAEYMKDPKRRRGLLMAFDGVQRLQQQISEKA
                     LADNVFIASGSGKPDTIFWSAVMKNRYNAMICIEKDACVLVEQDLNSDGRAERILFAF
                     NDERYIIYGIDPAGKEWKEITMNSLPAVITKEKLLMAAEDGKLGTKPKVWRDLTVDGE
                     RLDLNLNE"
     gene            28189..29811
                     /gene="pckA"
                     /locus_tag="ACHKIR_001158"
     CDS             28189..29811
                     /gene="pckA"
                     /locus_tag="ACHKIR_001158"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFO9988924.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTKQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIENIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTRILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            29926..30243
                     /locus_tag="ACHKIR_001159"
     CDS             29926..30243
                     /locus_tag="ACHKIR_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO9988925.1"
                     /translation="MFTFIELQGFSKRRQLLLPDDEFRAFQELLIQDPTAGDIIAGTG
                     SFRKIRWNRSGMGKRGGIRIIYYNVTRNGRIYLALIYPKNEQDDLTEEQRKALKLLSE
                     KLL"
     gene            30302..30598
                     /gene="nadS"
                     /locus_tag="ACHKIR_001160"
     CDS             30302..30598
                     /gene="nadS"
                     /locus_tag="ACHKIR_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="HFO9988926.1"
                     /translation="MKDELFADLLASAEEMVRIEKGEETPEPEHVHTFSEIDVKAIRE
                     ATGLRQQDFAVAVGVSYDLVKSWETKRRQPTGAPRKLLLLLQANPFIINQLKTI"
     gene            complement(30630..31982)
                     /gene="envZ"
                     /locus_tag="ACHKIR_001161"
     CDS             complement(30630..31982)
                     /gene="envZ"
                     /locus_tag="ACHKIR_001161"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HFO9988927.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFVSLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGSSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(31979..32698)
                     /gene="ompR"
                     /locus_tag="ACHKIR_001162"
     CDS             complement(31979..32698)
                     /gene="ompR"
                     /locus_tag="ACHKIR_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HFO9988928.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            32926..33402
                     /gene="greB"
                     /locus_tag="ACHKIR_001163"
     CDS             32926..33402
                     /gene="greB"
                     /locus_tag="ACHKIR_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HFO9988929.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            33499..35820
                     /gene="yhgF"
                     /locus_tag="ACHKIR_001164"
     CDS             33499..35820
                     /gene="yhgF"
                     /locus_tag="ACHKIR_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFO9988930.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDELANAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYIDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVNGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFEEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNDRPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            36258..36485
                     /gene="feoA"
                     /locus_tag="ACHKIR_001165"
     CDS             36258..36485
                     /gene="feoA"
                     /locus_tag="ACHKIR_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HFO9988931.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            36502..38823
                     /gene="feoB"
                     /locus_tag="ACHKIR_001166"
     CDS             36502..38823
                     /gene="feoB"
                     /locus_tag="ACHKIR_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HFO9988932.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGSRQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            38823..39059
                     /gene="feoC"
                     /locus_tag="ACHKIR_001167"
     CDS             38823..39059
                     /gene="feoC"
                     /locus_tag="ACHKIR_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HFO9988933.1"
                     /translation="MASLIQVRDLLALRGRMEAAQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGNCKSCPEGKACLREWWALR"
     gene            39262..40143
                     /gene="rpnA"
                     /locus_tag="ACHKIR_001168"
     CDS             39262..40143
                     /gene="rpnA"
                     /locus_tag="ACHKIR_001168"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HFO9988934.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAEHSPKHKESLMTIAERLRQEGFQKGIRAIAKTMLDAGVPLEDV
                     LRFTSLTVEDLAEINHQ"
     gene            40215..40334
                     /locus_tag="ACHKIR_001169"
                     /pseudo
     CDS             40215..40334
                     /locus_tag="ACHKIR_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312280.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(40363..41133)
                     /gene="bioH"
                     /locus_tag="ACHKIR_001170"
     CDS             complement(40363..41133)
                     /gene="bioH"
                     /locus_tag="ACHKIR_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HFO9988935.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGAMSLADMAEAVLRQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKMVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPAEFCRMLVALKQRV"
     gene            41171..41854
                     /gene="gntX"
                     /locus_tag="ACHKIR_001171"
     CDS             41171..41854
                     /gene="gntX"
                     /locus_tag="ACHKIR_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HFO9988936.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRAARTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALARLLLLEVL
                     HARRTTGLQLPDRIISVPLWQQRHWRRGFNQSDLLCQPLSRWLHCRWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            41913..42488
                     /gene="nfuA"
                     /locus_tag="ACHKIR_001172"
     CDS             41913..42488
                     /gene="nfuA"
                     /locus_tag="ACHKIR_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HFO9988937.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            42849..44165
                     /gene="gntT"
                     /locus_tag="ACHKIR_001173"
     CDS             42849..44165
                     /gene="gntT"
                     /locus_tag="ACHKIR_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFO9988938.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(44210..46294)
                     /gene="malQ"
                     /locus_tag="ACHKIR_001174"
     CDS             complement(44210..46294)
                     /gene="malQ"
                     /locus_tag="ACHKIR_001174"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HFO9988939.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTFTQEDQRVHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWKLPTTQQTLQQTRDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QVAAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGSETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMADPVNIPGTSYQYKNWRRKLSATLEAMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(46304..48697)
                     /gene="malP"
                     /locus_tag="ACHKIR_001175"
     CDS             complement(46304..48697)
                     /gene="malP"
                     /locus_tag="ACHKIR_001175"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HFO9988940.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELAEYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLKKEWTNDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPQVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            49319..52024
                     /gene="malT"
                     /locus_tag="ACHKIR_001176"
     CDS             49319..52024
                     /gene="malT"
                     /locus_tag="ACHKIR_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HFO9988941.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGDWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTSDKAAAANWLRHTAKPEFA
                     NNHFLQGQWHNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            complement(52067..53083)
                     /gene="rtcA"
                     /locus_tag="ACHKIR_001177"
     CDS             complement(52067..53083)
                     /gene="rtcA"
                     /locus_tag="ACHKIR_001177"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HFO9988942.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITGIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGDYRFAIGSAGSSTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKMGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVALFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFSLVEADGVTR
                     VSIE"
     gene            complement(53087..54313)
                     /gene="rtcB"
                     /locus_tag="ACHKIR_001178"
     CDS             complement(53087..54313)
                     /gene="rtcB"
                     /locus_tag="ACHKIR_001178"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HFO9988943.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPVNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESDQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQETLETLPSRD
                     LAYFMEGTEYFDDYLKAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKMFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            54502..56100
                     /gene="rtcR"
                     /locus_tag="ACHKIR_001179"
     CDS             54502..56100
                     /gene="rtcR"
                     /locus_tag="ACHKIR_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HFO9988944.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCLCQQESLVINRL
                     ELLHDARSRSLFETLKCDIASVSPETEVVSVEIELHNPWDFEEVYACLHDFARGYAFQ
                     PDKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSPPRKKEQPRGAGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEMNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLSQRQEDIEPNLDYEVERHASLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITREVVEDEINRLRYNWQES
                     RPSAITALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(56082..56840)
                     /locus_tag="ACHKIR_001180"
     CDS             complement(56082..56840)
                     /locus_tag="ACHKIR_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HFO9988945.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(56857..57687)
                     /gene="glpG"
                     /locus_tag="ACHKIR_001181"
     CDS             complement(56857..57687)
                     /gene="glpG"
                     /locus_tag="ACHKIR_001181"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HFO9988946.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(57732..58058)
                     /gene="glpE"
                     /locus_tag="ACHKIR_001182"
     CDS             complement(57732..58058)
                     /gene="glpE"
                     /locus_tag="ACHKIR_001182"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HFO9988947.1"
                     /translation="MEQFECINVAVAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWRRQFPA
                     EVAYGA"
     gene            58248..59753
                     /gene="glpD"
                     /locus_tag="ACHKIR_001183"
     CDS             58248..59753
                     /gene="glpD"
                     /locus_tag="ACHKIR_001183"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HFO9988948.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDDGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNTHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGTVSDLGEDFGHEFYEAE
                     LKYLVDHEWVRRADDALWRRTKQGMWLNAEQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(59807..60412)
                     /locus_tag="ACHKIR_001184"
     CDS             complement(59807..60412)
                     /locus_tag="ACHKIR_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9988949.1"
                     /translation="MIKFRLYIPPVILGFVIVPLLVWPTVIALAVLIFTLTFLAEIIF
                     SFPLLVVRISLQELQLELMVEYALFFSVMGGIGWQFSRRTPPELKNRLHYWLVFSPAY
                     FWLILSNFILYITPEKSALLENIRNFFLTFVWLPLNFSPFWPQPWTDFVGPISAQLGF
                     ALGYYCQWRSKNRSQGQKWVEWGTCLSLAILAQGPLFNYLQ"
     gene            complement(60415..61191)
                     /locus_tag="ACHKIR_001185"
     CDS             complement(60415..61191)
                     /locus_tag="ACHKIR_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9988950.1"
                     /translation="MQPSSAMQALIEQVYHIFRRYPVPQQFVVCCEYCLSQQEQKALR
                     NTSLRAIPYSLINAWNSSPGPDPQNSDEVRYFLPRLLEFVAQGQFDNIHEVFSLRRIN
                     LASKENWREDERAILQRFACQYMADWVSGDEAVELQYMLEMFSRADIALSPLLDAIIS
                     VPGYQPTVSMACLLWHNREENIQNSRFEQDDDDKAITAQINAWAFNNQFILKERARQA
                     IENLLNQPEQGTHYQVWEDDWMIDECLCAMYDASSESPGK"
     gene            complement(61194..61904)
                     /locus_tag="ACHKIR_001186"
     CDS             complement(61194..61904)
                     /locus_tag="ACHKIR_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9988951.1"
                     /translation="MELVDTLFASLSGTDPFTGVDITIANCKSTYWDEGIIQQLINQV
                     LDEGEKFAGTVGLEGLSRYDVTLNIGLTSSNVWPGFSLDTATIGRLCACGADFGFDPY
                     ISDVPDVQCDLNTTNDFTVHFTAMLNPDERVIIAKRPLKKCDSWIEDVYIFQVFKEAW
                     KFHTDNSLRGFRDKQAELKLYARHYSVENCTEESCRDCNYCIRPSFSLSRSSIIRLNA
                     ANARFVYQPFTRDQRKRG"
     gene            complement(62078..>62789)
                     /locus_tag="ACHKIR_001187"
     CDS             complement(62078..>62789)
                     /locus_tag="ACHKIR_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO9988952.1"
                     /translation="MTDKTVPFENYSTNYPADRSKADYWSSGTEGYIKIRLDKKIISD
                     VSLNNVLLVSLYASQRQTEHGPVPLFNAWISNLDIQVPQGCTINEGTSFTVNMPDVWA
                     SELSRAGAGAKPAGVTPVATTIPINCTNKDTDAVMTLVFDGNISATRDTNGKQSIIQA
                     QDNPDVGIMIMDSQQNSVDLNALATSVGVPFRLVENQAASAQTADVTFLALPVSTTGK
                     VPAAGRYNALAVLRVEYQ"
BASE COUNT        15223 a        17095 c        15656 g        14815 t
ORIGIN      
        1 gtactggcta cgttaactgc atgtactggt catattgaaa accgtgataa gaactgctct
       61 tacgactacc tgctgcaccc ggcaatttct atttctaaaa tcattggcgg ttgcggtcct
      121 actgcacagt aagtctctga ctgaaagcgg ctaacaccgg tttaattgcc tgacgccctc
      181 ggggttaccc gagggcgtat ttatttctgc attcttttcg aaaacatcat gcacaagatt
      241 aaatactggc aatactctca cgcaaaatcc gcagcaccgt ctcttcaccg ctgcgatttg
      301 ggttaatacg aatcgcgtaa tgttcgagtt gcggattcgc ctggcgaaac gttccggaaa
      361 gtcgataaaa aagtggcgga atttcatatt tcgactctgc accaaccggg taaggcaagg
      421 caccgcgctt ttgcgcctct tccagtactc tggcggcaat cggctgatga aactccacaa
      481 tcaacacctt cgactgcgca ttggcaatca ccgcgctttt cacttccgcc actgcgccac
      541 cgttaagtaa agccagcaac cgttcagata cccccgcctg caccgcgtgc atcactggcg
      601 cgagcaccag accacgcaac acctctaatg cctgcgcccc ctgaatctga ctgccgccgg
      661 aatagagcgt ggcgcgaatc cggctaataa catcggcatc gcctaccacc gcaccaacgc
      721 cctctggccc aaatagcttg aagcaggaaa atgtcgacac attcgccccg cattcgcagc
      781 caattcgcga caccttcatc accgcatagt tgtcatcggt taacgctgga acacctgccg
      841 cgcgcaacgt tgccagcacg tctgccagca catagcggtc ctgcggctgc tggcgcgtat
      901 gctgcaccag cgcggcatca ggttgttgct cgtcaacaac ctgcttcagt gccgacagat
      961 cattgaaatc agccgtaatg agcgttagcc ccatctgctc aataatgacg cgcgtcgttg
     1021 gataaaccgg cgcatcatgc accagaagac gttgccccgg tttgagcaaa gccgccagtg
     1081 ccgcacgaat cgcgccagta cccgcgccct gtaccagtgc cgccgcgggt gcatgaaacg
     1141 cgtcagccag cacctgttcc acacgctggg taatgcgggg ctgatttagc cccggcgtta
     1201 agcccaaatc accgcaggta agaaactcgc tgccaggaaa atgacggcaa atcgtgtcca
     1261 ccagcgcaaa ctgcttttgc tgcgcctcaa taagcgtcag gctttgcaga ggaaacgtct
     1321 tcataggtgc cttccttaag ccggaacgaa caggccaagc cagtaaagaa cattcagcag
     1381 gatgccggtg atcattactg ctacgaccgg tgccgccatt ttctgtaccg gacggcccag
     1441 ggattcgttg aggaagtaaa tggcaaccgc gatagagaat ccggtatagc ccgccatttt
     1501 gattgctgca aagatcgaac cgaccagcaa cgccacttcc atcagcatat tcatggcgtt
     1561 acggatgtta tccgacgcat tacgtaccga cgggtagcgc cccaaccatt tgccgatcga
     1621 acgaagcagc aagacttccg ccgaaataac cactgcgcct aataccgctg caaccatcgg
     1681 attcggcgag agatagccca ccgcataaac aaaggtaaag cccgcaactg catacacacc
     1741 agtggctaac gcggtggtgg caatcaacgg tacaaaaccc agcccgcgca taaattccgc
     1801 cagagctgcc tggttgatta gtgtttgcga ttgttccggc gttacgcctg cggaatatgc
     1861 tttctccagt gtgaagatcg acacttcact gccagcaaaa atcttcatgc tggcaacggc
     1921 ggcaatcagc gccccgacaa tagcgatata cggtaagttt ttgataatcc gtgacgtgcg
     1981 ttcttcaaac accgaaagcc cgctggcatc aatgtcattt tcatcacgat gacgcaggtc
     2041 gtgagtaatc gcgatcccca gcagcatcac catgccaata aagatttcga tggattccgg
     2101 attgagatgc gggaaatagc gcactactac cacacgggtc atcagtacca ccacggcggc
     2161 gatcagactt tgcttccagc caaactggta gaaaatcgcc accaacggga acagtgcaaa
     2221 agctgaaacc accggcgagc ttaattcccc caggctacct aatacatcca ccggtagcgc
     2281 ggtcagcagc tggtttaccg gtaacaggca agtaaggatc aacacgcccc agatagcgcc
     2341 aagaccaaac gccatcaggc tgtttatcgc cagtacgccg aggatatcgg taggaagaaa
     2401 gagtaaccat gcgttgagca gtccggtttt cagggtaaaa gagatcccca ccgaagccac
     2461 aaaaccaatg ctcagaccaa aagcgatact ccccgcctcg cgacggttca tatagccttc
     2521 aatcagttgc ggcaggatcg ggcggatacc gtcatgaaaa acggccgccg agcgatgcgc
     2581 cagaagcgat gtcatacccg tcaggcacgc caccacgata atctgaatat acagatccat
     2641 agtcgtgcct tttatttcag atggttaatc agcatcggaa tcgcgtgttc gacgtgctca
     2701 acggaaagcc caaacgccac tttcccttcg gcgatcattt tggcgatatg ttcgtctttc
     2761 gctttaatgc ctggtttggc aatggtgcaa cttttgttat agccaatcac cgcgatggca
     2821 atcgacaatg ccgcgcccgc accggtatta cacgcgccga tgtagtaatc cagttgcccg
     2881 gatttgactt tcattgccgc ttccatgtcg ttgtgaatga aaacttcaaa acagccagga
     2941 gccgtcgctt caatggtttt tttaatctgc tcacgctgta agcctgcaac accaatcttt
     3001 ttcatgagtc tgtccttatt ggaaaaattg agagggagtt tcacgtaaca tcacatccac
     3061 atcggcctga ctgaatcctg actggcgcaa ttgcggaata aaggtggtca ataaatagtc
     3121 atagccataa ccaccgttgg cttttaaatg ggagcggcgc gtaatatcca tcgacagcat
     3181 gacgcggtta agcaaacctc ggtcacgtag cgcgtggagc atcgcaatgc gcttttcgtc
     3241 cgggtagtaa ctgttcttac cgatggtgtc gaactgcaca tacgcgccga gatcgatcat
     3301 cttcacaatg ttgtcgaggt tgtctttcag atcgcagtga ccaacggtga cgcgcgaaag
     3361 atcaactccg tgggcttgca gtagcgccag ttgttccagc cccatggtgc taaaagaggt
     3421 atgcgtggag atcgggcgtc cggtctggtt atgcgccagc gcggcggcaa taaatacctt
     3481 ctcttccagc ggcgtgattt ttccttcgct ggagcctatc tccgcgatga tcccggcttt
     3541 cagctccgtg ccatcgatac cctgttcaat ttcatcgacc atctcttgcg ccagttcctg
     3601 cacgctgcgg gtcgccacat gttccgggaa aaacgcgtcc tggtaataac cggtacaggc
     3661 caccacgttg atcccggttt cgcgcattac atcgagcata aattgcgcat tgcgccccat
     3721 gtaacggttg gtcatctcaa tcacattacg cacgccctgg gccatcaggt cgttcatctc
     3781 ctggcaaatg aacgcatact gatcaaggcg gcagtccacg ttgtttttaa agccggagag
     3841 atcaatatgc agatgctcat gggcgagggt gtagcccgtc ggatcaaaac tcatagggtg
     3901 tctcctgcaa accggaggga ggaaagaaaa gagcgaccat tttgtggcgg tggcgcgcgg
     3961 aaaaattcac atacggtagc ccccacatca gaaagcgtgg tgagcacacc aagctgcgta
     4021 gcgaccatcc cttgctgata aaccagcact ggcaccactt cgcgggtatg gtggctgtga
     4081 ccaatggtcg gatcgttacc gtgatccgcc atcacgacca ggcaatcatc tggctgcatc
     4141 gcctcaataa gccgggcaag gttacggtca acgacctgca aacgttccgc ataacgtgcg
     4201 acgtcttctg catgaccagc gaggtcggtt tcctggatat tggtacaaat aaacgccgtc
     4261 ggatgggtgt taaattcgtt gaaggtgata tccataatcc gctggctatc caccaggttt
     4321 tgccagctca cgccataagg attactggca atatccgcca ctttccccac cagcacggtt
     4381 ggtacgccca cttcatacag tttttgtggc acctgcactt tttcatcgac gccgtagccc
     4441 atatgcacga cctggaagcc gttgtcataa gcgccagaac gcggcgcatt gatgccaata
     4501 aagcgccctt ctttgctttc tgcggcatcg aggatgcgct ggctgtcggt taacaggcca
     4561 ccaaatgtaa tgacccgacc gacctgtacc tgctcacgca cgatacgacc aattttgatt
     4621 gcgtcgtcaa aagagatcac agagagattg gcggtgatgt tatagacctg acctaaatcc
     4681 gcctcgagat tatcgccaat cgcaaccgcc tgattgaccc acagaaattg cagatcatcg
     4741 ccacggcgct ccacctgcca gtcagcgtta agtaatgctt gctcaacacg gtcaatcaca
     4801 tcgcgaaagg gcatccgtag cggcggtaac gggcgcgtgc ctaaaatttc ctgatgcccc
     4861 ataaaggtat cgccaccttc atgttgcagc tctgccacgc cccaggttgc ggagtctgac
     4921 ggctgcatat ctccaggcgc ataacctaat gcgttgatta gccccatttt ctccagcatt
     4981 ggtagctgca aatgcggcaa ctggctcagg atgtgaccac aggtatttgc cccggcatcc
     5041 tgcggacgca ccaacgtgac atctttcatt gcccctacgc caaagctatc aatcactaac
     5101 accacaaatc gcgccattac gcccccagag agtttccaag actgtcataa cgaccgacga
     5161 tttctggttc gccacggtga ataccggaca gcaacaccac atcgctacgg gtgacaaaaa
     5221 tctgcgtgcg aaaacagagc actactgcac tgcttaccgg aaactcgcct gccagcggca
     5281 gggtgtagtc gatactgctg tcatccacag ttttgagatt ggtttcagta atcttttgat
     5341 tttctggcgt aaacaccagc gcatgttgcg cgtgaccacg acggtagtaa ccgccgccgt
     5401 agcagtagct gtcgccacga aaatgatggg agatttcact taaccagagc atcgcgatac
     5461 gttcaggctg atcgccctgc tggtttgccg gaatcgtccc cgtcagcgca tgaccgggtt
     5521 cggcatgagt cacaccgtat tccgccagca atggcagcga agtgcagctg gtcgcagaag
     5581 gtgcgttcag ttgctcaatc gcaataccag atttcgccag ttgatcccgt gcctgtatca
     5641 gcgtgtgaag attcggtgtc ggcaaaactt tcccggcagc ctcatcccaa agcaggcaag
     5701 ggaaatgggt tagtccggct aaatgcagcc ccggcagttt cttaatatca gcaaccacct
     5761 cgtgcagcga atgctgtaca aaaccgctct cctgccccgg ataaagaaaa tcatcgtcgc
     5821 tataaacttt gagcagcaca gactgcacac gtccggcctt caccgctgct gcagaaactt
     5881 cccgcgcttt atcgagagta aacacggtga tgacgtcagt tccctgtgca acggcgtcag
     5941 aaacctggtg acaagggatt tgcaccagat gcccctgatg cgccaacggc agaccggcgc
     6001 ggcgcatgac ccgcgcctct ttgtaatcca ccgccacaat gccgctgtag cccagtgcca
     6061 gaagtttttc cgccagccac ggattacggc caaactgctt ggtcatcaga tacagttcaa
     6121 tgccgtaaag tcgcgccgtc tcaatcagcc gcttaccgtt ttccagtatc tgatccacat
     6181 cgatcaccca gctgtccggg gcgatcttgc cctgctgcca caaacttagt gcggcggaaa
     6241 tcagcgccgg gttctggcgt ttcaatgctt ctacaaacat cttgttgcct cgtttctgtt
     6301 ccttgaatta aatattcatt tttctgaata tttagattat gcgacgtgga tttgtcggga
     6361 gcttcaaacc tcttcgttag cagccatcca taatccataa agattagcca gcaaatagcc
     6421 ttcttcacac ggcttaactt ccagtgcgaa ttccttcaac aacacctgat gcaattgcac
     6481 aacggcttgc cagtggctgg attgcgccag ctctgccagc aattcgtcat ccagcggctc
     6541 tatttcttca ccacggcgac tgcgcatcag tgcgctcgcc atatgtgtca tcgccatcat
     6601 tccttgctcg ctgcgcaccg gtagatgaca ctctgtttcc agtacgttga cgacctgcat
     6661 catgcctttg cagatgtcct tatcaataac gcctgcctca caaagcaggt tgagtctggt
     6721 ttccatttca gatgcctttc gtttaataac tcggttcctg ttccccactg accactcgtt
     6781 gttgatgggc aagcagggcc tgtttatcaa caactgcacg cagcaattgt tgcattgggt
     6841 aatcatcgac tcgcgtcaaa actacggctt cggtggcctg taaaaatcgc ggatcgtctg
     6901 tgagcggcgt cgcttctaat cccagcatgg tcagttcgtt ttccgccacc acgttccaga
     6961 tcaccgcatc aacatcgcct ttaacaatgc gttgtaaact ctcgtgataa gagagatcga
     7021 ctcgttccac atcactatcg ccaaaaaaga catcggtcat gattttctga tccgccgaac
     7081 ggttatccag ccccacgcga ttcacgtttg cggactcgcc tttacggcaa atcaactggt
     7141 gctcgccaac gtaggtgtgc ggccccaact ccagcgcgag gcataaccct ttttgcgtga
     7201 gataactttc cgccgccagt cgggaaacaa ccgccatgtc atacacgccg ttaagtaaac
     7261 actccacgcg aatatccgcg ccgcgcatgt gcgcatagta aaaaggaata ccatcaaact
     7321 gagctttcaa tccgctcgcc aggccttcgt acaaacgggt atagggcaag ggcatcgcgc
     7381 acaccacatt gttgatatcc acatgactca gcaatgcttt gttatccatt tccaccagat
     7441 aactgccatt acgcccacgg cgttctatcc gtatcgctcc gcttgattcc agcgttttca
     7501 acgcggcctg cgtcaggcca acggatgaac ggcattcagt tgccagttca tctatggttt
     7561 tcagtcgatt accgcacttt tcacccaaca aataacgggc cagtgtcgtg atgacgacgc
     7621 cttctttttt gataaacgtt cgacgcataa taaattttca gtaaattgaa tatttatatc
     7681 ttcaggaatt tgaagataag gcgcaacagc gagatgtgga aacggcgagg cacttcacat
     7741 tttttcggat tattgcaaat gaggataaaa aaaccgggtt tccccggttt cagagtgatg
     7801 ataaaagcaa aattgcctga tgcgctacgc ttatcaggcc tacatttcct tgcaatatgt
     7861 gcattacttt gtaggccgga taaggcgttc acgccgcatc cggcatgaac aaagcgcaat
     7921 ttgccagcaa tagtgaatta cggcttcgcc acaaaaccaa tcgcttcgta caccgctttc
     7981 agcgtacgag aagcgtgtgc actggctttt tccgcgccat ctttcatcac ctgttgcagg
     8041 aaggcttcgt cattgcggaa acggtgatag cgttcctgca attcagtcag cataccggaa
     8101 acggcatcag ccacttcgcc tttcagatga ccatacatct tgccttcgaa ctgtttttcc
     8161 agttccggga tgctctggcc cgttaccgca gaaaggatat ccagcaggtt ggaaacgccc
     8221 gctttgttct gcacatcgta gcgaactacc ggcggctcgt cggagtcagt gaccgcacgt
     8281 ttgattttct tcactaccga tttcggatct tccagcaggc cgataacgtt attgcggtta
     8341 tcgtcagact tggacatctt tttggtcggc tccagcagcg acattacgcg cgcgccagat
     8401 ttcggaataa acggctccgg cactttaaag atctcgccat acagcgcgtt gaaacgctgg
     8461 gcgatatcgc ggctcagttc caggtgctgt ttctggtctt caccgaccgg taccagatta
     8521 gtttgataca gcaggatgtc cgcagccatc agcaccggat agtcaaacag accagcattg
     8581 atgttctcgg cataacgcgc agatttatct ttaaactgcg tcatgcggct cagttcgccg
     8641 aagtaggtat agcagttcag tgcccagcct aactgtgcat gttccggcac gtgggactga
     8701 acaaaaatgg tgcttttctc aggatcgata ccacaagcca gatacaaggc cagcgtatcc
     8761 agcgtcgctt tacgcagctt ctgtgcatcc tggcgcacgg tgatcgcgtg ttggtcaacg
     8821 atacagtaaa tgcaatgata gtcatcctgc atgtttaccc actgacgcag cgcacccatg
     8881 tagttaccaa tggtcaattc acctgagggc tgtgcgccac taaaaacgat gggcttagtc
     8941 atttttcgat tcctgatttt cgctatgcgg aagccctaat gcgggcagaa ggtcatttat
     9001 agactgataa attacatcag gctggctgag atcgatagcc tcgccgtagt tatatccgta
     9061 ggttaagcca actgatgggc aacctgccgc ttttgctgcc tgaatatcat tgcgtgagtc
     9121 gccaacaaac agcatctgtt gtggggcaat tcccatccgc tcagccacca gtaacagcgg
     9181 gtccggatgc ggttttttgt tttgcacatc atcaccgccg ataaccacgc tgaagtattt
     9241 ggcgatatct aaggcttcga gcagcggcgc gacgaacggc gtcggtttgt tggtgaccag
     9301 gcctagcggc aggcctttag cctgcaatgc gcccagcgta tcggcaacgt gcgggaacaa
     9361 aaacgtcccc tcttcggcaa cctcgccata gtagcgatcg aacagtttac gcagaatacg
     9421 tacctgttct tctgccggaa tgtcgtcatc aacgggcggt ttacccattg ttttacgcag
     9481 agtcgcacgt tcctgacgcg cccaggtcaa tgcgcgctcc atcagaatat ctgcgccgtt
     9541 accaatccag gtaataacgc gttcttcacc tgcgacaggc aactccagcg catacagcgc
     9601 catatctacc gcagcagcaa gaccgggtgc actgtcaacc agcgtaccgt cgagatcaaa
     9661 agcgacgccg cgaatatctt caaacttatt catgacttac ctttgccagt tcactgcgca
     9721 tttcatcaat gacttttttg tagtctggct ggtcgaagat tgccgaacca gcaacaaaca
     9781 tatccgcgcc cgccgcagcg atttcgccaa tgttgttcac cttcacgcca ccgtccactt
     9841 ccaatcgaat gtcaaagcca gactcgtcga tacgacgacg tacttcgcgc agtttatcca
     9901 gtgtttgagg aatgaaagac tgaccgccga aaccaggatt gacggacatc agcaggatca
     9961 catccagctt atccatcacg taatccagat aactcagagg tgtcgccggg ttaaatacca
    10021 gacccgcttt acagccattt tctttaatca gttgcagcgt gcggtcaaca tgctcggagg
    10081 cttctggatg aaaggtaatg atgctggcac cagcggcagc gaaatcaggc acaatgcgat
    10141 cgacgggttt caccatcagg tgtacgtcga taggggcggt aatgccgtag ttacgcaagg
    10201 atttcagcac cattggccca atagtcagat tgggaacata gtggttatcc atgacgtcaa
    10261 aatgcacgac atcagcgcca gctgccaggg cttttgcggt atcttcaccc aggcgggcaa
    10321 aatcagccga cagaattgag ggggcaatca aatactgttt catccgcttc tccttgagaa
    10381 ttattttttc gcgggtgaaa cgactcctgg tttgtacaaa gccagcagtt cgtccacctt
    10441 tttacgtgtg ccgccgttgc tgcttatact gcgtcgaact ttgacgacat gcaattttgc
    10501 gcgctgatac cactcacgcg ttaacatcgt atcgtgattg gagatcagca ctggaatatg
    10561 gcgatcaacc agaccttcgg cgatctccgc cagatgcgct tgttgttcaa gcgtaaaact
    10621 gtttgtgtga tacgccgtaa agttggcggt cgcagacagc ggcgcataag gcggatcgca
    10681 atagacgacg gatgcatcat ctgcgcgcgc catgctatcg gcgtaagact cacagtagaa
    10741 aaaggcattc tgcgcttttt cagcgaagtg atacaactct gcttccggga aatagggttt
    10801 tttgtagcgg ccaaacggca cgttaaactc accgcgcaga ttgtaacgac acagaccgtt
    10861 gtaaccgtag cggttcaaat ataaaaacag taccgcccga cggaacggat cctggctttt
    10921 gttgaactct tcgcggaact gatagtaaac ctcggcgcaa tttgtttcgg gaacaaacag
    10981 ctcgcgtgcg gcctgtacgt actcatcagt acgcatcttc acaatgttat agagactgat
    11041 caggtcgcta ttgatatcgg caaggatata acgagaaaag tcggtgttga gaaacaccga
    11101 cccggcacct acaaaaggct caaccagaca ttcgcccttg ggcaaatgcc gtttaatatc
    11161 atcaagcagg ggatacttgc cccctgccca cttcaaaaaa gcgcgatttt tcttcatgct
    11221 gactaactaa ttacaccttc tccggctgtg gagaaagctc cgacagcatc cagcgcttca
    11281 ggcaactccc gcgatgataa accattgcgg gagataacct tgattacttc agatcggcct
    11341 gtacctgacg cagcggtttc gcccacgggt ttttggcctg gacatcggct ggcaatgtag
    11401 ataccgcttt tttcgcctct tctttcgaag cgtacacgcc agaaaccagg acataccacg
    11461 gctgaccatt acgcgtcgtt tcatagacaa cgtagttttt cagattctct ttcttcgccc
    11521 aaccgttcag gttgtcgtag ttagaggaac tgctcagctg cagagtgtaa tggctggacg
    11581 gtgccgattt caacgaacca acattacctg cggtcttccc tccagcggct ggtgttgccg
    11641 tggtttgcgc cggggatgcc gtctgtactg gagccgtagt cgccgtctct tttggtgctg
    11701 gcgtagaagt cgcagccggt gccttcgtgc ttgccacagg agcagctggt tcagtacgct
    11761 tcggcgtctg tgctaccggc ttcggctccg ttttcacggt tgcttgcggt tttttcggtt
    11821 caatcaccgc ctgctggcga gctggacgcg tagtggccgg acgttcagcg gtttgcgttt
    11881 tcgccgtgtc acgcgatgca ttgccattgc gaacaggcgc gaccgtcgcg ggttcagtcg
    11941 gcaacgtgga attgaccgcc acattattca actgttgctg attttgtggc tgggtcagcg
    12001 cattgttcag gtcaccctgc acttcaacac gttgttgacc atccgttgcc gccggggttt
    12061 gcccttgagt cggcgtagaa gagatcggcg gcagagaaac atcctgctga gtattttccg
    12121 cagacgtggt tcccggcgct ggctgcacgc cattcgcctg atcggtcgca ttgccagcaa
    12181 gatcaatact cttctcgcca gacgcggttt gatcgctgga agaggtcgag ggggctttta
    12241 gcgcagaacc gataccgatg atcaacagca ggagaaccag aatgccgacg cccatcatca
    12301 tatactgacg agaagcgggt ttactggctg cttttttgcg cttacgtgga cgacgctcta
    12361 cgcgctcttc atccacggtt tcatcttcgg attcgtcaat ttcttcttcg agttccggtt
    12421 cctcattgcg ctctttttgc gcacgagtcg gacggcgatc gtcagtgtca tcaagttcaa
    12481 tatcatcaaa attgatctgc ggttcgccac gttcagtacg ctcagaacgt tcagaagatt
    12541 gacgagaacg accagtacga cgatcgctgg gatcgggttt cagctcgtct tctggtttga
    12601 attcatccat ttaacacccc actaaaaggt taatgcttac cacgttgcaa ttaacctgaa
    12661 gctaatagac cgcttgataa gcggcctgac ctttcttgtt gttacgctga ttgacaatcg
    12721 gcaatggcgt taagaacaag ctcgtgcgaa acgccgctgc gaacttcact cttaccaatt
    12781 gccaacggaa gaattaagcg catctctccc gcaaggactt tcttgtcacg cagcatatgc
    12841 ggtaaatacg cctgcgcaga catttcgcgc ggcccattga ccggtaaccc agcccgcgtg
    12901 agcagggtta taatacgctg cgtttcggca gaactaaact gcccgagacg ttccgacgtc
    12961 cgcgccgcca tcaccatacc cgcagcaacc gcttcaccgt gtaaccaatt gccatacccc
    13021 atttcagctt caatggcatg accaaaggtg tgtcccagat tcagtaaagc acgtaacccg
    13081 gtttcgcgct cgtcggcggc gacaacttct gccttcagtt cacaacaacg gcgaatacag
    13141 tacgccattg ccggaccgtc cagacgcaac aacgcatcca gattctcttc cagccagttg
    13201 aaaaacgcac cgtcaagaat aatgccgtat ttgatgactt ctgccagccc cgacgctaac
    13261 tcacgcgggg gaagcgtttt cagacagtcg agatccacca ccactgaagc aggctggtag
    13321 aacgcgccaa tcatgttttt accgagggga tggttgaccg cagttttgcc gccaacggag
    13381 gaatcgacct gcgacagcaa tgtcgtcggg acttgaatga aacgtacacc gcgctgataa
    13441 ctcgccgccg caaagccggt cagatcgccc actacgccgc cgccaagcgc caccagcgta
    13501 gtatcgcgac catgcggctt ttgtaacaac gccgtaaaga cggtatcgag tacagccagg
    13561 cttttatact gctcgccgtc agggaggata acgctatcga cgttaacacc cgcctgttca
    13621 agtacgccgc ggaccttatc gagatacagg ggagccaggg tttcgttggt aaccaacatg
    13681 acctgctcgc ccgatttcag cggtaagaat gaagctggtt cattaaacaa accagatgcg
    13741 atggtaattg ggtaactacg ttccccgaga gtaacgacaa tcctctccat aacgcgacat
    13801 ccaccttaat tactgtaccc gcagacgagt gtatataaag ccagaattag ttgctttcca
    13861 gcatgtgaat aatctggttt gcaaccactt tagcgctttg atcatcagta cgaatggtca
    13921 cgtcggcaat ctcttcatac agcggattgc gttcattggc caacgcttcc agaacttcac
    13981 gcggcggtgt ttcaacgtgc agcaacgggc gttttttatc acgctgcgtg cgtgcaagtt
    14041 gcttttcgat ggtcgtttca agataaacga caacgccacg agcggaaaga cggttacgcg
    14101 tttcacggga tttcacagag ccgccgccag tagccagcac aataccctgt ttctcggtca
    14161 actcattgat gaccttttct tcgcgatcgc ggaagccttc ttcgccttct aaatcgaaaa
    14221 cccagcccac atcagctccg gttcgtttct caatctcttg atcggaatcg taaaattcca
    14281 tattgagttg ttgagctaac tggcgcccaa tagtgctttt tccggcaccc ataggcccaa
    14341 ccagaaagat attgcgtttc tctgccattt tttcggtact actaagacta ttcgttaatg
    14401 ataaacccgc ttcgctcaga gagcgccgca ggacatgaac tgaaacctca taagatattg
    14461 cgagagtcag actgaaaatt atctcaatac tccagcgggt ttggcaactg aataaatcac
    14521 ccatccctct gcatatctgg tcgctgcaag cgcgctgcct tgctaccacc gctctggcga
    14581 ttaatcaccg ggtaagatta gcgtaaaaaa gacagcaaaa tgccgcctga atgataaatc
    14641 atcatcatgg aacgcaggca gcgaagagca ccaactctca aatcggtact ccttgtatgc
    14701 taaatacctg cgcgcgtcaa atagatgaaa cacgttcagc gtaaaaacat taccgctttt
    14761 acggctgttt actcactgga aaccagtcgt ggcgtgataa acaccactaa ctcgcgtcgt
    14821 tcatcttctt ttccgtcatg acgaaataat tgcccgaacc agggaatgtc gccaagcaac
    14881 ggtacgctat cctgacccga tttattttta cgggtaaaaa tgccgcccag cgccaacgtt
    14941 tctccgcttt tgacctcgac ctgcgtttcg atctcctgct tatcaatcgc caacacttcg
    15001 ccatcggcct gctgtagcac ctgccccgga acgttctggc tgatgtgtaa tttcagccgg
    15061 atgcgacctt tttgtaacac cgtgggcgta acttccatcc ccaggacggc ctctttaaat
    15121 tccaccgacg tcgcgccact ttccccgctg gaaacctgat atggaatttc gctcccctgt
    15181 ttaatgctgg caggctgaag atgtgaggcc agcagacgcg gactggcgat aatatccagc
    15241 tgctgttttt gttcgagcgc ggaaagctca agatccagta aacgtccgtt gatgcgtcca
    15301 atgttaaaac cgacatgcgt tgtcgccgtc gctacggaga ggtcgctgcc aagcgtggtg
    15361 acttgcccaa cgccaccagc ttgctgcgca tcagccaacg tccatttcac gcctaactca
    15421 cgcaaacttt tttcattaat ggtgacaata tgcgccgaca gctcaacctg cccgaccggc
    15481 agatccattt gcgctaccca ctgttcaagc gcgcttaacg ccgttttgtt atctcgcagc
    15541 aaaaggcgat tggtgcgttt atcgacggtc atactccctt tggcactcag tagcttctcc
    15601 ccggctttcg ccagttctcc cgcgtcggcg tattgcaggg ttatactgcg attttccagc
    15661 ggcagatttg cctgcgcccg cgcctgctcc gcctcctgac gggcgatatt gtcattctgc
    15721 caggcaacgg aatgcaccga gagaatgttg ccctcctggc gcgttatcaa tccggcgctt
    15781 ttcactacag tttgtagtgc ctgcttccag ggaacgtcag ttaaatgtaa cgacaccgta
    15841 ccgctgacgt ctggcgaaac caccaggttc aacttctcct gttcagccag cgcctgcaac
    15901 acctgagcta ccggaacgtc atccaccatc agcgtcactt tttgcggctt tgccgcctgt
    15961 acgccgggta taagcatcaa cagtagtgcg gctatccatt gcttcatttg tatctccttg
    16021 ccgttgccac aaccagtgtg gcggttcgca gtttgtcccg gttcccagcg ttagtgcgtc
    16081 tggcgtcagc tgtaaaattg tccagccgtt ttccagcacg tcgttttgct gcacccgtcg
    16141 ccattttttt tgcccgtctt ttattacacc gatgatgcgc tcacctcgcc ctaccatccc
    16201 ctgatagcgc cactggctaa gttcgctaat ccggcataga tcttccggcg gtttaaaagg
    16261 gtcacgcata ccggttaaaa ggcacaatgt aatacctgcc aacaaccagc gtttaaccct
    16321 catgcggcgt ctccagttgt agcgtgaaca aaaggtcatc accttccacg cttaacgaaa
    16381 aacggctcac gctgacgttg cgctctgcca gccgtgtaaa tgccgacggc actgcttccc
    16441 acagcgtttt caacgccaac tcacctccct gcgcggatgg atgccagtaa accagttgcg
    16501 cgccggataa ctgaaaatcc agtggcgaaa agggcagcgt tttttcctcg ctgaagggag
    16561 tggtgtcaac caggcgatac agtgcggccc actgctggtg atgacttgcc cgtagtcgaa
    16621 ttaatgcgtc gctctcttca tggtgtgtcg atgacagaaa aatgagcgta actaacatca
    16681 gcaaccagac tgcccagcaa agctggcgga ggcggggtga tgtggcgaac caccagtcaa
    16741 agagcatgtc catcgctaac cttccttgtt aactgatact caaattgcca gcgtccctgc
    16801 gcatcctgct gcgtggcccc ccgctgattg agatgaaaag atgcatcctg gcggagtgac
    16861 gtttccagcg cgtttaacgc ggtaatgctt gttgtcagcc ccttgatctc cagcgtacct
    16921 tgctgccagc ttatcgttgt cagccaggca tgttcgggta aaagcgccgc cagtgcttcc
    16981 agcgcagatt gccagtcacg ggtaaattgt cgctggagct gcctttgcaa acgctgttcg
    17041 cgtaactgct ggcgttccag caaacgtggt ttcgttatct gtaagctgcg ggcgagttgt
    17101 tgttccgcct gaagcaaaac ggtgtctacg cgtgcttcgg cgttgcttgt cagacgcagt
    17161 attagcgtta tcccgacagc cagcagcaga ggcgcaacga acatcagcaa ccagaaacgc
    17221 agaaaagcgg tccggcgttg ctgtcgccag ggcaaaaaat taattggcgg gttcatcagt
    17281 acgccttccc tagcgccagc cccagcgcga tggcaaagtc tccacctggc ggcggtagcg
    17341 gcggctgacg aacagaaacc gcctcccagg gatcaaatcc gccttcgcca catatcgcga
    17401 cgctttcggg atcaacggat aacgctgccg ccagctcctt cgcgctagtc atccccaccg
    17461 ccagtttgcg cccccagcga tagcgcgtcg cccacagcca ctgttcgtta tcacgccagg
    17521 ccagacattg ctgatgagaa ggtaaaaaag gcaggaatcg ctgtaatgca ctggcatccg
    17581 gggtgatcgc gctaacatga acacgcaacc tttctgccag agtaagcagc gttgccaact
    17641 ctttgctttg cgcggcagtc acgttatagg cgggactgag tgagtcttcg ctgtaatcga
    17701 agcgcaggga gtccggatcc atatccagct cgcgggccat cgtcccggac agccaggccg
    17761 tttgctcccg ctcaccaagg gacatcgacg gacgtggaaa tgaccgctgt aatgtgcgac
    17821 tggcgggaaa cgccaacata atgtgatgac gctgcggcag ttcgcgactc cacggtaaca
    17881 acgttttagc cagccgctgc acatcaacaa tccgcccatc tttgataatg tcgttctcca
    17941 gcggcaaccg ccaccagcgt tgcaaaaagc attcttttgt gccccaaccg ctaccgcttc
    18001 ttgctgttgt aaatgcaaac caatttgcca gatcttaaat gccattgtga tgatctcctt
    18061 atcacccgtc actctgacgg gtatatcaat gcgtctggct tgcctttata ctaccgcgcg
    18121 tttgtttata aactgcccaa atgaaactaa atgggaaatt tccagtgaag ttcgtaaagt
    18181 attttttgat ccttgcagtc tgttgcattc tgctgggagc aggctcgatt tatggcctat
    18241 accgctacat cgagccacaa ctgccggatg tggcgacatt aaaagatgtt cgcctgcaaa
    18301 ttccgatgca gatttacagc gccgatggcg agctgattgc tcaatacggt gagaaacgtc
    18361 gtattccggt tacgttggat caaatcccgc cggagatggt gaaagccttt atcgcgacgg
    18421 aagacagccg cttctacgag catcacggcg ttgacccggt ggggatcttc cgtgcggcaa
    18481 gtgtggcgct gttctccggc cacgcgtcac aaggggcgag taccattacc cagcagctgg
    18541 cgagaaactt cttcctcagt ccagaacgca cgctgatgcg taagattaag gaagtcttcc
    18601 tcgcgattcg cattgaacag ttgctgacga aagacgagat cctcgagctt tatctgaaca
    18661 agatttacct cggttaccgc gcctatggtg tcggtgctgc ggcacaagtc tatttcggta
    18721 aaacggtcga ccaactgacg ctgaacgaaa tggcggtgat agccgggctg ccgaaagcgc
    18781 cttccacctt taatccgctc tactcaatgg atcgtgccgt cgcgcggcgt aacgtcgtac
    18841 tgtcgcggat gctggatgaa gggtatatca cccaacaaca gttcgatcag acacgcactg
    18901 aggcgattaa cgctaactat cacgcgccgg agattgcttt ctctgcgccg tacctgagcg
    18961 aaatggtgcg ccaggagatg tataaccgtt atggcgaaag tgcctatgaa gacggttatc
    19021 gcatttacac caccatcacc cgcaaagtgc agcaggccgc acagcaggcg gtacgtaata
    19081 acgtgctgga ctacgacatg cgccacggct atcgcggccc ggcaaatgtg ctgtggaaag
    19141 tgggcgagtc ggcgtgggat aacaacaaga ttaccgatac gctgaaggcg ctgccgacct
    19201 atggtccgct gctgcctgcc gcagtcacca gcgccaatcc tcaggaagcg acggcgatgc
    19261 tggcggacgg gtcgaccgtc gcattgagta tggaaggcgt tcgctgggcg cgtccttacc
    19321 gttcggatac tcagcaggga ccgacgccgc gtaaagtgac cgatgttctg caaacgggtc
    19381 agcaaatctg ggttcgtcag gttggcgatg catggtggct ggcgcaagtg ccggaagtga
    19441 actcggcgct ggtgtcgatc aatccgcaaa acggtgccgt tatggcgctg gtcggtggct
    19501 ttgatttcaa tcagagcaag tttaaccgcg ccacccaggc actgcgtcag gtaggttcca
    19561 acatcaaacc gttcctctac accgcggcga tggataaagg tctgacgctg gcaagtatgt
    19621 tgaacgatgt gccaatttcc cgctgggatg caggtgccgg ttctgactgg cagccgaaga
    19681 actcaccacc gcagtatgcc ggtccaattc gcttacgtca gggactgggc cagtcgaaaa
    19741 acgtggtgat ggtacgcgca atgcgggcga tgggcgtcga ctacgctgca gaatatctgc
    19801 aacgcttcgg cttcccggcg caaaacattg tccacaccga atcgctggcg ctgggttcag
    19861 cgtcattcac cccaatgcag gtggcgcgcg gctacgcggt catggcgaac ggcggcttcc
    19921 tggtggaccc gtggtttatc agcaaaattg aaaacgatca gggcggcgtg attttcgaag
    19981 cgaaaccgaa agtagcctgc ccggaatgcg atattccggt gatttacggt gatacgcaga
    20041 aatcgaacgt gctggaaaat aacgatgttg aagatgtcgc tatctcccgc gagcagcaga
    20101 atgtttctgt accaatgccg cagctggagc aggcaaatca ggcgttagtg gcgaagaccg
    20161 gcgcgcagga atacgcaccg cacgtcatca acactccgct ggcattcctg attaagagtg
    20221 ccttgaacac caatattttt ggtgaacccg gctggcaggg tactggctgg cgagcaggtc
    20281 gtgatttaca acggcatgat ataggcggaa aaacgggtac cactaacagt tcgaaagatg
    20341 cgtggttctc gggttacggt ccgggcgttg tgacctcggt ctggattggc tttgatgatc
    20401 accgtcgtaa tctcggtcat acaacagctt ccggagcgat taaagatcag atctcaggtt
    20461 acgaaggcgg tgccaagagt gcccaacctg catgggacgc ttatatgaaa gccgttcttg
    20521 aaggtgtgcc ggagcagccg ctgacgccgc cgccgggtat tgtgacggtg aatatcgatc
    20581 gcagcaccgg gcagttagct aatggtggca acagccgcga agagtatttc atcgaaggta
    20641 cgcagccgac acaacaggcg gtgcacgagg tgggaacgac cattatcgat aatggcgagg
    20701 cacaggaatt gttctgatta aaaaggcgct tcagcgcctt tttaagtttg ctgacaaagt
    20761 gcactttgtt tataccggat acggcgtgaa cactttaccc gtctaaaaac atctagaaaa
    20821 atcaatatat tgcaggagcc acacaggcct gataagcatt atgcatcagg caaacttcac
    20881 gcatttacaa ccgcccctgc cccttcaacc attcgcgcac gaggaaaagc gcgctgacat
    20941 tacgcgcttc gttgaagtca gggtcttcca gcaaatccat catatgcgcc agcggccagc
    21001 gcacctgtgg tagcggctct ggctcatcgc cttccagcga ttccgggtag agatcttgcg
    21061 ctaccacgat attcattttg ctggaaaagt aagacggtgc catgctgagc ttcttcaaaa
    21121 aagtcagatc gttcgcacca aatcccacct cttcttttag ctcgcggttc gcggcttcga
    21181 agacgctttc acccggatca attaatcctt tcgaaaaacc taattcgtag gattcagttc
    21241 ccactgcgta ttcgcggatc aggatcaggt gatcgtccac aatcggcaca atcatcactg
    21301 cttcccggtt ggttggacgc attcgttcat aaacacgccg cacgccattg ctgaactcca
    21361 gatccacgct ttcgacggta aacagtcggg aacgggctac agtttcaaca ttcagaatgg
    21421 tgggtttttg taatgatttg ctcatcgtgg gatctatgct gtgaaatcag cggttattgt
    21481 gcgatatcgg acacgctttc ggcaatgtga attgcatgtt atttacattt atgtaactta
    21541 ataaataatc gtcctcaaat caaattaaaa gtcaataggt tgaaataact ccaggaattt
    21601 gctgatattc cgccttcaga gggtttgcta tgatcagcgg ttactgtgat gtgcttaatg
    21661 atgctcaagt taaactccac gcttgccgat agccaaccgc agaatcatgt tttgtgtccg
    21721 gtgcgactga ccacgcctga cagactaagt aagatgggga aagcatgagc accattgtga
    21781 tttttttagc tgctttgctg gcctgctcac tacttgcggg atggctgata aaagtgcgat
    21841 ccagacggcg tcagctgccc tggaccaacg ccttcgcgga tgcgcaaacg cgtaaactca
    21901 cacctgaaga acgtagcgcc gttgaaaatt atcttgagag cctgacgcag gtattacagg
    21961 tgcctggccc aacgggagcc agcgcggcac cgatttctct ggcgctgaat gccgaaagca
    22021 acaacgtcat gatgctaaca cacgctatca cgcgttacgg catctctacc gacgatccga
    22081 ataaatggcg ttactacctc gattcggtag aagtccacct gccccctttc tgggagcagt
    22141 acatcaacga tgagaatacc gttgaactga ttcataccga ttcgctgccg ctggttattt
    22201 cactcaacgg tcatacgctg caggagtaca tgcaggaaac tcgcggctat gccttgcaac
    22261 ctgttccgtc aacacaggcg tcgattcgcg gggaagagag tgagcaaatc gagctactca
    22321 atattcgcaa agaaacgcac gaagagtatg cgctaagtcg tccgcgcggg ctgcgcgaag
    22381 cgttgctgat tgtcgcctcc ttcctgatgt tctttttctg cctgattacc ccggatgtat
    22441 ttgttccgtg gctggcaggc ggcgcgttac tgctgctggg cgcaggtctg tgggggctat
    22501 tcgcgccccc ggcaaaatcc tccctgcggg aaattcattg tctgcgcggt acaccccgtc
    22561 gttggggatt gtttggcgaa aacgatcagg aacagatcaa caatatttca ctcggtatta
    22621 tcgacctggt ctaccccgca cactggcagc cctacattgc tcatgatctc ggccaacaaa
    22681 ccgatatcga tatctatctc gaccgccacg tagtgcgtca ggggcgatat ctttcgctgc
    22741 atgatgaagt aaaaaacttt ccgttacagc actggctgcg cagtacgatt atcgctgcgg
    22801 gttcgctgct ggtgctgttt atgctgttat tctggatccc gctggatatg ccgctgaaat
    22861 tcactctctc atggatgaaa ggcgcgcaga ccattgaagc caccagcgtg aaacaactgg
    22921 ctgacgctgg cgtacgggtt ggcgatacat tgcgtattag cggtacggga atgtgtaata
    22981 ttcgcacttc cggtacctgg agcgcgaaaa ccaactcacc ttttttaccg tttgactgct
    23041 cgcagatcat ctggaatgac gcccgctcat tgccattacc agaatctgaa ttggtcaaca
    23101 aagcgacggc attgaccgaa gcagttaatc gccagctgca ccctaaaccg gaagatgaat
    23161 ctcgcgtcag cgcctcatta cgttcagcaa ttcaaaaatc cggcatggta ttgcttgatg
    23221 attttggcga catagtactg aagacagcgg atctgtgttc tgccaaagat gactgtgtgc
    23281 gactgaaaaa tgcgctggtc aatctcggca acagtaaaga ctgggacgcg ctggtaaaac
    23341 gcgccaacgc cgggaaactc gatggcgtga atgtgttatt acgcccggtg agtgcggaat
    23401 cgctggataa cctggtggca acctccaccg cgccgtttat cacgcatgaa acggcgcgag
    23461 cggcacaatc actaaacagt ccggcccccg gcggattcct gattgtcagc gacgaaggca
    23521 gcgattttgt tgatcagccc tggccttcgg catcacttta cgactacccg ccgcaagaac
    23581 agtggaacgc tttccagaaa ctggcacaaa tgctgatgca tacgccgttt aacgccgagg
    23641 gtatcgtcac aaaaatcttc actgacgcca atggtacgca gcatataggc cttcatccga
    23701 tcccggatcg ttccggcctg tggcgctatc tcagcaccac gctgctgcta ctgacgatgc
    23761 tgggtagcgc catttataat ggcgtacagg cctggcggcg ttaccagcgt catcgtactc
    23821 gcatgatgaa gattcaggcc tattatgaaa gctgcctgaa cccacaactg atcacccctt
    23881 cagaaagcct tatcgaataa cacgtttgcg cggcaggtta tgctaccctg tcgcgcaaat
    23941 tgcttcactc tggagatttc cctcatgcat atcaacattg cctggcagga cgtagatacc
    24001 gttctgctgg atatggacgg cacgttgctc gacctcgcct tcgataacta tttctggcaa
    24061 aagctggtgc ctgaaacatg gggcgcgaaa aacggggtta cgccacagga agcgatggaa
    24121 tatatgcgcc agcaatatca cgacgtacag catacgctaa actggtactg tcttgattac
    24181 tggagtgagc aactgggtct ggatatctgt gcgatgacca ccgagatggg accgcgcgcc
    24241 gtactgcgtg aagataccat tccgtttctt gaggcactga aagccagcgg taagcagcga
    24301 attttgctca ccaatgcgca tccgcacaac ctggcggtaa aacttgagca taccggtctg
    24361 gacgcacacc ttgatttatt actttccacc cacacatttg gttatccgaa agaggatcag
    24421 cggttatggc atgcggtggc cgaagctacg ggtctgaaag ctgaaagaac gctgtttatt
    24481 gatgacagcg aagcgattct cgatgctgcc gcgcaatttg gtattcgtta ctgcctcggc
    24541 gtgactaatc ctgactccgg gattgccgag aaacagtatc aacgccatcc gtcactgaat
    24601 gactaccgcc gcctgatccc ctcgctaatg tgaaggagac gccatgaaag aaaaacctgc
    24661 tgttgaggtt cgactggata aatggctatg ggctgcccgt ttttataaaa cccgcgcgct
    24721 ggcccgtgaa atgattgaag gcggtaaggt gcattacaac gggcagcgca gcaagccgag
    24781 caaaatcgtc gagctgaatg ccacgctcac tctgcgccag ggaaatgacg aacgcacggt
    24841 gattgtaaag gcgattactg aacagcgtcg ccccgccagc gaggcagcct tgctgtatga
    24901 agagactgcg gaaagtgtag agaaacgcga aaaaatggcg ctggcacgta aacttaatgc
    24961 cttaaccatg ccgcacccgg accgacgccc ggacaaaaaa gagcgccgcg acctgttacg
    25021 atttaaacac ggcgacagtg aataactgtc acctgcaaga gagatgatta tgccgcaaca
    25081 tgaccaatta catcgctatc tgtttgaaaa ctttgccgtt cgcggcgaac tggtaaccgt
    25141 ttcggaaacc ctgcaacaga tccttgagaa ccacgattat ccgcagcccg ttaaaaacgt
    25201 gctggcagaa ctgctggttg cgaccagcct gttaaccgct acgctgaagt ttgatggtga
    25261 tatcaccgta cagctgcaag gcgacggtcc gatgaatctg gcggttatta acggtaacaa
    25321 caaccagcag atgcgcggtg tggcgcgcgt gcagggcgaa attccagaaa atgccgacct
    25381 gaaaacgctg gtcggcaatg gttacgtggt gatcaccatc accccgagcg aaggtgaacg
    25441 ctatcagggc gtggtgggtc tggaaggtga tactctggcg gcctgcctgg aagattactt
    25501 tatgcgttct gaacagctgc cgacgcgcct gtttattcgc accggcgacg tagacggcaa
    25561 accggctgca ggcggtatgt tgttgcaggt aatgcctgcg caaaatgccc agcaggacga
    25621 ctttgaccac ctggcgacgc taaccgaaac catcaaaacc gaagaactgt tgaccttacc
    25681 ggcaaacgaa gtgttgtggc gtttgtatca cgaagaagag gtgacggttt atgatccgca
    25741 ggatgtggag ttcaaatgca cctgctcgcg tgaacgttgc gccgacgcgc taaaaacgct
    25801 gccggatgaa gaagtcgata gcatcctggc ggaagatggc gaaattgaca tgcattgtga
    25861 ttactgcggt aaccactatc tgttcaatgc gatggatatt gctgaaatcc gcaacaacgc
    25921 gtctccggca gatccgcaag ttcattaatg agtatgtccg gcagagaggg ctctgccgga
    25981 ttttacgacg taatggcctg atgcgcttat cagacctacg gattcaatgc aatttatcgc
    26041 aggtaagata atgtgccgaa tccaataaaa atcagatatg cgctgtcatt cattcagatt
    26101 caaatccagt ctttcgccat ccaccgtaag atcgcgccac actttaggct tcgtccccag
    26161 ttttccatcc tccgcagcca tgagtaattt ttctttcgtt attacagccg ggagtgaatt
    26221 catcgtaatc tctttccatt ctttcccagc aggatcaata ccatagataa tgtatctttc
    26281 atcattaaag gcaaacagga tccgctctgc cctgccatca ctattcagat cttgctctac
    26341 cagcacgcag gcatcttttt caatgcatat cattgcgtta tagcgatttt tcatcactgc
    26401 cgaccagaat atcgtatccg gttttccaga acctgaggca ataaatacat tatctgctaa
    26461 tgctttttcc gatatctgct gctgaagacg ttgtactcca tcaaatgcca tcagcagacc
    26521 ccgcctgcgt ttcgggtctt tcatatactc ggcatcgctt ttcagcgact caagcgccgc
    26581 acgtccatag cgaccgctct gttcgagcat atagagactt acctggtctg gcgtgttttt
    26641 gccgctctga taacgcgcca tatgactgtt cacgctaatg cgcatactgt ccagcaccgg
    26701 agaattaaga agcaccagta ttaccaacac caataacgaa accgcaaggt tcactttgcc
    26761 ctgaagaaca agggggtttt gccctttacg ccagacgata ctgacaaaat accccagcga
    26821 ccataccagt aacaccagca ccgccagcgc gccctgcaag cggtcgacag tccagccgta
    26881 ctgagcgacc cgcagccata acgcccaggc ggcgacgaac acatacagcg gagcaaccag
    26941 caaagcggtt ttaatcaggc aacgcaacgg ccccgtccag ggaagagacg ctttttgcgg
    27001 atcgcggaca atagccatta agatcaattg caaaaaggcc agcgttaaca gcaacccagc
    27061 ggcggagatg tggcgagaaa tcgcgctcag gcccgtaaac ggcagggtga taataaacat
    27121 cagggttagc aatgatacta acggcagcaa ccccgtggcg attaacgtga acaacttttg
    27181 aatagagtcg attaaacgtg actgtgtacg cgcgaggatc accgccagtg cggtaaccag
    27241 acctaacgtg agataaataa accagtcggt tgcaaaaaag agcgttttga aaaacgtgat
    27301 gccaacaagt ttaaacaact ctccccacag taaaagtacc agccacgtca ggccattggc
    27361 gagaaaaatc accagtaata taagtacgtt atgccatact gactggtaaa aatagcgata
    27421 gcggctactg tcattgcgga tgcgaaggct ttgttgtatc cacggtagca acaacattgc
    27481 catcagcagc agatagcaac caaaatccca aagcgccttt tctgctctcc aggggttcat
    27541 gccatcaatt tgccacttta gccaaccgct catccctagc gtggcaataa acaccagcgc
    27601 cagccatccc caaaggcgct tctgtttaaa ggagatcacg gagaagagta aaacggatga
    27661 aaacgcgacg gtcgcaggca cgccataaac aatccaactg tgattttttc cggcaagcca
    27721 ggcgatcagc aggtagcaaa ccagaccctg aagtaatccg gtagcaatca tcccccagcg
    27781 tgttgccggt gaaagttcaa cgttatccat gttagttatc cagaatcaaa aagtgggtta
    27841 attattgcat ccgggccgta gtattttgct tttttcagaa aataatcaaa aaaagttagc
    27901 gtggtgaatc gatactttac cgattgaatt tgcatcaatt tcattcagga atgcgattcc
    27961 actcacaata ttcccgccat ataaaccaag atttaacctt ttaagaacat tttccacacc
    28021 taaaatgcta tttctgcgat aatagcaacc gtttcgtgac aggaatcacg gagttttttg
    28081 tcaaatatga atttctccag atacgtaaat ctatgagcct tgtcgcggtt aacaccccca
    28141 aaaagacttt actattcagg caatacatat tggctaagga gcagtgaaat gcgcgttaac
    28201 aatggtttga ccccgcaaga actcgaggct tatggtatca gtgacgtaca tgatatcgtt
    28261 tacaacccaa gctacgacct gctgtatcag gaagagctcg atccgagcct gacaggttat
    28321 gagcgcgggg tgttaactaa tctgggtgcc gttgccgtcg ataccgggat cttcaccggt
    28381 cgttcaccaa aagataagta tatcgtccgt gacgatacca ctcgcgatac tttctggtgg
    28441 gcagacaaag gcaaaggtaa gaacgacaac aaacctctct ctccggaaac ctggcagcat
    28501 ctgaaaggcc tggtgaccaa acagctttcc ggcaaacgtc tgttcgttgt cgacgccttc
    28561 tgtggtgcga acccggatac tcgtctttcc gtccgtttca tcaccgaagt ggcctggcag
    28621 gcgcattttg tcaaaaacat gtttattcgc ccgagcgatg aagagctggc aggtttcaaa
    28681 ccagacttta tcgttatgaa cggcgcgaag tgcactaacc cgcagtggaa agaacagggt
    28741 ctgaactccg aaaacttcgt ggcgtttaac ctgaccgagc gcatgcagct gattggcggc
    28801 acctggtacg gcggcgaaat gaagaaaggg atgttctcaa tgatgaacta cctgctgccg
    28861 ctgaaaggta tcgcttctat gcactgctcc gccaacgttg gcgagaaagg cgatgttgcg
    28921 gtgttcttcg gcctttccgg caccggtaaa accacccttt ccaccgaccc gaaacgtcgc
    28981 ctgattggcg atgacgagca cggctgggac gatgacggtg tgtttaactt tgaaggcggc
    29041 tgctacgcaa aaactatcaa gctgtcgaaa gaagcggaac ctgaaatcta caacgctatc
    29101 cgtcgtgatg cgctgctgga aaacgtcacc gtgcgtgaag atggcactat cgactttgat
    29161 gatggttcaa aaaccgagaa cacccgcgtt tcttatccga tctatcacat cgaaaacatc
    29221 gttaagccgg tgtccaaagc gggccacgca actaaagtta tcttcctgac cgcagacgcc
    29281 tttggtgtgc tgccgccggt ttctcgtctg actgccgatc aaacccagta ccacttcctc
    29341 tctggcttca ccgccaaact ggccggtaca gagcgcggca tcaccgaacc gacgccaacc
    29401 ttctccgcct gcttcggcgc ggcattcctg tcgctgcacc cgacgcagta cgcagaagtg
    29461 ctggtgaaac gtatgcaggc ggcaggcgcg caggcgtatc tggttaacac cggctggaac
    29521 ggcactggca aacgtatctc cattaaagat acccgcgcca ttatcgacgc catcctcaac
    29581 ggttcgctgg ataacgcaga aaccttcact ctgccgatgt ttaacctggc gatcccaacc
    29641 gaactgccgg gcgtagacac caggatcctc gatccgcgta acacctacgc ctctccggaa
    29701 cagtggcagg aaaaagctga aacgctggcg aaactgttta tcgacaactt cgataaatac
    29761 accgacaccc ctgcgggtgc cgcgctggta gcggcaggtc ctaagctgta atcagctgat
    29821 atctcattga ccccttatcc aaacggataa ggggtttttt tatctaatta gctaccaata
    29881 tgtacacggt acacttataa ttatcaatag accgcaggat gcattatgtt cacctttatt
    29941 gaactacaag gatttagcaa acgccgccag ttattgctac ctgatgatga gttccgggct
    30001 tttcaggaac tactcattca agatcccact gccggtgaca ttatcgcagg cacaggcagt
    30061 tttagaaaaa ttcgctggaa ccgttctgga atggggaaac ggggtggcat tcggataatt
    30121 tattacaacg taacacgcaa tggccgaatt tatctggcgt tgatttatcc taaaaatgaa
    30181 caggatgatt taacagaaga acaaagaaag gcattgaaac tattgtccga gaagctgctt
    30241 taatgttgaa gctccacaaa ttccaccatg gccgaaatgt caacggaggc attgaaaaca
    30301 gatgaaagat gaattatttg ctgatcttct tgccagcgca gaagaaatgg tacgcatcga
    30361 aaagggtgaa gaaacgcctg aaccagagca cgtccatact tttagcgaaa tcgatgtaaa
    30421 agcgatccgt gaagcaacgg gtttacgtca gcaagatttt gctgtcgccg taggggtaag
    30481 ttatgacctg gtcaaaagct gggaaaccaa acgccgtcaa cctaccggag caccccgaaa
    30541 attgctcctg cttttgcaag cgaatccttt cattatcaat caattgaaaa caatctaaaa
    30601 acaggaggca ccttcgcctc ccgttttatt tacccttctt ttgtcgtgcc ctgcgcccgc
    30661 gttaccggca ctggcagcca ggcgcgaatg gaaagcccgc cccgctcgct gctgccaagc
    30721 tccagcatgc cgttatggtt atccacgata cgctgcacaa tcgccagccc taatcccgtg
    30781 ccgctaatgg tgcgcgcact gtcgccgcga acaaacggct ggaacaggtg cttacgttgt
    30841 tccggcgcaa ttcccggacc atcatcttcc acctggaacc aggcgcgatt cggctccgtt
    30901 ccgctgctga ctttgatcca gccattgcca tagcgggcag cgttcaccac catattcgcc
    30961 accgcacgtt tgatcgacag cgggtgcatt ttcacctcaa tgctgccggg gtaaagcgcg
    31021 gtttcaattt cccgctcata gccactttcg gcggcaatca cctcaccgag cactgcgttg
    31081 agatccgcca tttccatcgg catctcctgc ccggtgcgca ggtagtcgat aaactgctca
    31141 atgatggcgt tgcactcttc gatatcttta ttgatcgatt cggccaggta gccatcctgc
    31201 tcgctcatca tctcggtcgc caggcgaatg cgcgtcagcg gcgtacgcag atcatgactt
    31261 acccccgcca tcagcagcgt gcgatcatcc gccagttgct taacaccagc cgccatatgg
    31321 ttaaaggcgc gggtaacgga acgcacctcc gaagcgccat attcacgcag cggcggcgga
    31381 ataatccctt taccgacctg cagagcagca tgttcgagat cgaccaacgg tcggttctgg
    31441 atacggataa acagccacgc cccgcctatc gccaatagca taatcgccag cgtatagcgg
    31501 aacagcggag aaaaatcacc ctgatgaatt tcggtcagcg gcacgcgcac ccagatattg
    31561 ggcgacagcc aggttttcag ccagacaaca ggcgaacttt tgttgacctc aacgcgcact
    31621 tccgtcgggc cgcccagttg ctgcgccatc tgatggctta agaattcata gtgttgtgcc
    31681 caacgcagac ctgcctcttc ggcagcttcg ttggaataga gagagatccc cagctcacgg
    31741 tagatctccc gacggaaagc gggaggcaca accaactgcg tgccgtcctc cagttgcagt
    31801 ttgtcggtca tcaacatacg cacttcgtac gcgaggactt tattaaactg ctggaggctc
    31861 ggcaaaatcg cgaagttcag caccaccaga taagtcgtca ccaggctgac gaacagcaag
    31921 gtgacgatga gcaataacgt acgggcaaat gaacttcgtg gcgagaagcg caatcgcctc
    31981 atgctttaga accgtccggt acaaagacgt agcccagacc ccagacggtc tgaatgtaac
    32041 gcggatgcgc cggatcttct tccaccatgc ggcgtagacg cgaaatctgc acgtcgatgg
    32101 agcgttccat tgcggagtat tcacggccac gggcaaggtt catcagctta tcgcgggaga
    32161 gcggctcacg cggatggctg accagtgcct tcagtaccgc aaactcaccg ctggtgagcg
    32221 gcatcggctc atcttcgcgg aacatttcac gcgtaccgag gttaagtttg aacttaccga
    32281 aagcaattac cgcctcttcc tgtgacggcg cgcccggcag ttcgttggcc tgacgacgca
    32341 gcaccgcacg aatgcgggcc agcaattcac gcgggttaaa cggttttgga atgtagtcgt
    32401 cagcgccaat ctccaggcca acgatacggt ccacttcttc ccctttcgcc gtcaccataa
    32461 tgatcggcat cgggttgctc tggctacgaa gacgtcggca aatcgacaag ccatcttcac
    32521 caggtaacat caaatccagt accataagat ggaaagattc acgagtcagc aggcgatcca
    32581 tctgttctgc attagcgacg cttcgaacct ggaagccttg ttcggtgaga taacgttcca
    32641 gcagcgcgcg caggcgcata tcgtcatcga ccaccagaat cttgtagttc tcttgcattg
    32701 tttgtactcc caaaggttcg caacaatttg taagcgtgta ttcttaaaaa agctcacgtt
    32761 cgtcaccagc taaatctggt atgaatttca gcctaaattg ttacaaagca tattaaacag
    32821 cagcttaagt atacaattta ttcggcgaaa cattattgat tctgttgata tgatcacgtt
    32881 atacccaatg tgcgcattat caaacagaca aagggaatca acgagatgaa aacgcccctg
    32941 gttacccggg aagggtatga aaaactcaaa caagagctta attatctctg gcgtgaagaa
    33001 cgcccggagg tcacaaaaaa ggtgacctgg gccgcaagtc tgggcgaccg cagcgaaaat
    33061 gctgactatc agtataataa aaagcgcctg cgtgaaatcg accgtcgcgt gcgctatctc
    33121 actaaatgca tggaaaatct caaaatcgtc gattactccc ctcagcagga aggcaaagtc
    33181 ttttttggtg cgtgggtgga gattgaaaac gacgatggcg tgactcaccg tttccgtatt
    33241 gtcggctacg atgaaatttt tggccgtaaa gattacatct ctatcgattc cccgatggct
    33301 cgcgcattgt tgaaaaaaga ggtgggcgat ttggcggtgg tgaatacccc tgccggggaa
    33361 gcgagctggt atgtgaatgc tatcgagtac gtgaaaccgt aaggaagagt cttaacctcc
    33421 tgccgatggc tggcattttt gccagccagt ccgtataact atcccctgat ttttgatccg
    33481 aaaagatgaa ctcaaaccat gatgaatgat tcgttctgcc gcattattgc gggtgaaatt
    33541 caggcgcgcc cggaacaggt tgacgctgcc gttcgcctgc ttgacgaagg gaataccgtg
    33601 ccgtttatcg cacgttatcg taaggaaatc accggcggtc tggatgacac gcagctgcgt
    33661 aatctggaaa cgcgtctgag ctatctgcgc gagctggaag agagacgtca ggcgatcctc
    33721 aagtccattt ccgagcaagg caaactcact gacgagctgg cgaatgccat caacgccaca
    33781 ctaagcaaaa ccgaactcga agacctctac ctgccctaca aacctaaacg ccgcacccgc
    33841 gggcaaatcg ccattgaagc agggcttgag ccgttggctg acctgctgtg gagcgatccg
    33901 tcacacacgc cagaagtcgc cgctgcacaa tatattgatg ccgataaagg cgtggcagat
    33961 accaaagccg cgctggacgg cgcgcgctat atcctaatgg aacggtttgc cgaagatgcc
    34021 gcgctgctgg cgaaagtgcg tgattatctg tggaagaacg cgcatttggt ctcaaccgtg
    34081 gtgaatggta aagaagagga aggggcgaaa ttccgcgact atttcgatca tcacgaaccg
    34141 ctgtccacgg tgccttctca ccgcgcgctg gcgatgttcc gtggtcgtaa cgaaggcgta
    34201 cttcagcttt cgctgaatgc cgatccgcag ttcgaagagc cgccgaaaga gagctattgc
    34261 gagcaaatca tcatggatca ccttggcctg cgcctgaaca atgccccggc ggatagctgg
    34321 cgcaaaggtg tggtgagctg gacgtggcgc atcaaggtgt tgatgcatct ggaaactgaa
    34381 ctgatgggca ccgtgcgcga acgcgcggaa gatgaagcaa tcaacgtctt tgcccgtaac
    34441 ctgcacgatc tgctgatggc ggcccctgcc ggactgcgtg caacgatggg cctcgatccg
    34501 ggtctgcgta ccggggtaaa agtggcggta gtcgatgcga caggtaaact ggtggcgacc
    34561 gataccattt atccgcatac cgggcaggcc gcaaaagcag cgatgaccgt tgctgcgctg
    34621 tgtgaaaagc ataacgttga actggtggcg atcggcaacg gtacagcttc tcgcgaaacc
    34681 gaacgtttct atctcgacgt gcagaagcag ttcccgaaag tgaccgcgca gaaagtgatc
    34741 gtcagcgaag ctggcgcgtc ggtttactcg gcttccgagc tggcagcaca ggaattcccg
    34801 gatctcgacg tttcgctgcg tggcgcggtg tctatcgccc gtcgtttgca ggatccgctg
    34861 gcggagctgg tgaaaatcga tccgaaatct atcggcgtag gtcagtatca gcatgacgtc
    34921 agccagacgc agctggcacg caaactggat gcagtagtag aagactgcgt aaacgccgtt
    34981 ggcgtcgatc tcaacaccgc ttctgttcct ctgttaaccc gcgtggcggg cctgacgcgc
    35041 atgatggcgc aaaacatcgt ggcctggcgc gatgagaacg gccagttcca gaaccgtcag
    35101 caactgttaa aagtgagccg tctggggccg aaagccttcg agcagtgcgc gggcttcttg
    35161 cgcattaacc atggtgataa cccgctggac gcgtctaccg tccacccgga agcctatccg
    35221 gtggtggaac gcattctggc agcaacacag caggcactga aagatctgat gggtaacagc
    35281 agcgagctgc gtaacctgaa agcgtctgac tttactgatg aaaaattcgg tgtgccgacg
    35341 gtaaccgaca tcatcaaaga gctggaaaaa ccgggtcgcg atccgcgtcc ggaatttaaa
    35401 accgctcagt ttgccgatgg cgtcgagaca atgaacgacc tgcaaccggg tatgatcctc
    35461 gaaggtgcgg tgaccaacgt caccaacttt ggcgcgtttg tcgatattgg cgtgcatcag
    35521 gacggcctgg ttcacatctc ttcattgtcg aacaagtttg tggaagatcc gcataccgtg
    35581 gtgaaagcgg gcgacattgt gaaggtgaaa gtgctggaag tggatcttca gcgtaaacgt
    35641 atcgccctga ccatgcgtct ggacgagcag cctggcgaaa ccaacgcccg tcgcggtggc
    35701 ggtaacgatc gcccgcaaaa caaccgcccg gcagccaaac cgcgtgggcg tgaagcgcag
    35761 cctgccggta atagcgcgat gatggatgcg ctggcggcgg caatgggcaa aaaacgttaa
    35821 acgcccggac tggcccctaa acctgcgcga ttaggcgttc acgccacatc tggcaacgaa
    35881 cgccttgcct gacataaaag tgccggagaa tatctccggc ctttttattc cacaagcaaa
    35941 ctcataatat atcccattaa tatttatcac ttcattaaca actgaaacct taattaaaca
    36001 ttaaccagcc ctaataattc gtcatgcgaa ttatattttc gctgcgatat aaccttgagc
    36061 catatcaaca ttgagtcaga ttattattca aaccaacatt cacacacatt ttaagtattg
    36121 ctgatagaaa ccattctcat tatcattgta ctgttgatta tttaatctct ccttcgttgg
    36181 caaatcatct ggtctcatgt cgctgtcaaa cgccccatga ggtagttatc cagttaatga
    36241 gaaacaagta ggcacctatg caatacactc cagatactgc gtggaaaatt actggctttt
    36301 cccgtgaaat cagcccggca tatcgccaaa aactgctttc tcttggcatg ttacctggct
    36361 cctcttttaa tgtggtgcgc gtcgctccac tcggcgaccc cattcatatc gaaacccgtc
    36421 gtgtgagcct ggtattacga aaaaaagatc tggccttatt agaagtggaa gcggtttcct
    36481 gttaatacgg tgataacaac aatgaaaaaa ttaaccattg gcttaattgg taatccaaat
    36541 tctggcaaga caacgttatt taaccagctc actggctcac gtcagcgtgt aggtaactgg
    36601 gctggcgtta ccgtcgaacg taaagaaggg caattctcca ccaccgatca tcaggtcacg
    36661 ctggtggacc tgcccggcac ctattctctg accaccatct catcgcagac ctcgctcgat
    36721 gagcaaatcg cctgtcacta cattttgagt ggcgacgcag acctgctgat taacgtggtg
    36781 gatgcgtcta accttgagcg taacctgtac ctgacgctgc aactgttgga actcggcatt
    36841 ccctgcatcg tggcactgaa catgctcgac attgccgaga agcaaaatat tcgtattgaa
    36901 attgatgctc tgtcggcgcg tcttggctgc ccggtgatcc cgctggtttc aacccgtggt
    36961 cgcggtattg aagcgctcaa gctggcgatt gatcgctata aagctaacga gaatgtggaa
    37021 ctggtgcatt acgcacagcc gctactcaac gaagcagatt cactggcaaa agtgatgcct
    37081 tccgacatcc cgctgaaaca acgtcgctgg ctgggcctgc aaatgctgga aggcgatatc
    37141 tacagccgcg cctacgccgg tgaagcgtcg cagcatctgg atgccgccct cgcccgtctg
    37201 cgtaatgaga tggacgatcc ggcgctgcac attgccgatg cgcgttacca gtgcattgct
    37261 gccatctgtg atgtggtaag caacaccctg acggcagaac ccagccgttt caccactgcg
    37321 gtagataaaa tcgtgctcaa ccgtttcctc ggtctgccga ttttcctctt tgtgatgtac
    37381 ctgatgttcc tgctggctat caacatcggc ggggcgttac agccgctgtt tgatgtcggt
    37441 tccgtggcgc tgtttgtgca tggtattcag tggattggct acacgctcca cttcccggac
    37501 tggctgacta tcttcctcgc ccagggcctg ggtggcggta ttaacaccgt gctgccactg
    37561 gtgccgcaga tcggcatgat gtacctgttc ctctccttcc ttgaggactc cggctatatg
    37621 gcgcgtgcgg cgtttgtgat ggaccgcctg atgcaggcgc tgggcttgcc ggggaaatcc
    37681 tttgtaccgc tgatcgtcgg cttcggttgt aacgtaccat cggtaatggg cgcacgtacg
    37741 cttgatgcac cgcgtgaacg cctgatgacc atcatgatgg caccgtttat gtcctgcggc
    37801 gcacgtctgg ctatcttcgc agtattcgcg gctgccttct tcgggcagaa cggtgcgctg
    37861 gcggtcttct cgctgtatat gctgggtatc gtaatggcgg tgctgacggg cctgatgctc
    37921 aagtacacca tcatgcgcgg tgaagcgacg ccgtttgtca tggagctgcc ggtctaccat
    37981 gtaccacacg ttaaaagcct gattatccag acctggcagc gtctgaaagg cttcgttctg
    38041 cgtgctggta aagtgattat catcgtcagc attttcctga gcgccttcaa cagcttctcg
    38101 ctgagcggaa aaatcgtcga taacatcaac gactcggcgc tggcttccgt cagccgggtg
    38161 atcaccccag tcttcaagcc gattggcgtg catgaagata actggcaggc aacggttggc
    38221 ctgtttacag gcgcaatggc gaaagaagtg gtggtgggca cgctcaacac cctctacacc
    38281 gcagaaaata ttcaggacga agagttcaat ccggcagaat ttaacctcgg tgaagagctg
    38341 ttcagcgcgg tagatgaaac ctggcagagc ctgaaagaca ccttcagcct tagcgtactg
    38401 atgaatccca ttgaagcgag caaaggcgac ggcgaaatgg ggaccggggc gatgggcgtg
    38461 atggatcaga aattcggtag cgcagctgcc gcatacagct acctgatttt cgtcctgctg
    38521 tatgtaccgt gcatctcggt gatgggggct atcgcccgtg aatcaagccg tggctggatg
    38581 ggcttctcca tcctgtgggg gctgaatatc gcttactcac tggcaacatt gttctatcag
    38641 gtcgccagct acagccagca cccaacttac agcctggtgt gcattctggc ggttatcctg
    38701 tttaacatcg tggttatcgg tttgctgcgt cgcgcgcgta gccgggtgga tatcgaactg
    38761 ctggcaacgc gcaagtcggt aagcagttgc tgcgcagcca gcaccaccgg tgattgccat
    38821 taatggcttc acttattcag gtgcgtgatt tgctggcgtt acggggccgt atggaagcgg
    38881 cccagataag ccagacattg aacaccccgc agccaatgat taacgccatg ctgcaacagc
    38941 tggaaagcat gggcaaagcc gtgcggattc aggaagaacc tgacggctgc ctctctggca
    39001 attgtaaaag ctgcccggaa ggcaaagcct gcctgcgcga gtggtgggcg ctgcgttaac
    39061 catactccat cacctgtttt gaaaagcccg gtatgcgttt gcatccgggc ttttttgcgt
    39121 gcggctttcc ataaaaatgc aacccttgca gcgcgacgta aattcctctg aaagcgtctc
    39181 gcagggatga aaactcgcta atgcacaggt gtggagtggt gcgtagagtc gcggcattca
    39241 aacaacaggt gaaggaacgc catgagcaaa aagcagagtt ccaccccaca cgatgcgctg
    39301 ttcaaactct ttttacgcca accggagacg gcgcgcgatt ttcttgcgtt tcatttaccg
    39361 gcacctattc acgcgctctg tgatatgaaa accctcaagc tggagtcgag cagctttatt
    39421 gatgacgatc tgcgtgaaag ctattccgac gtgctgtggt cggtgaaaac ggaacaaggg
    39481 ccaggatata tctattgtct gattgaacat caaagcacct caaacaaact gatcgcattt
    39541 cgcatgatgc gttacgccat tgccgcaatg caaaatcacc tggatgccgg atacaaaaca
    39601 ttgccgatgg tggtgccatt gttgttttac cacggtattg aaagccccta tccctattcg
    39661 ctgtgttggc ttgattgttt cgccgatccc aaactggcaa ggcagcttta tgcctccgca
    39721 tttccgctga ttgatgtcac cgtcatgcct gatgatgaaa tcatgcagca ccgacgtatg
    39781 gcgctgctgg agttaattca aaaacatatt cgtcaacgcg atctgatggg gctggtagag
    39841 caaatggcct gcttattaag tagtggatac gctaatgaca gacaaatcaa agggctgttt
    39901 aattacatct tgcaaaccgg cgatgcggta cgttttaacg attttatcga cggcgttgcc
    39961 gaacattcac cgaaacacaa ggagagctta atgactattg cggaaagatt gcggcaagag
    40021 gggtttcaga aaggtatacg agctatcgcc aaaaccatgc tggatgccgg agtccctctt
    40081 gaggacgttc tgcgatttac cagcctgacg gttgaagacc ttgctgaaat aaatcatcaa
    40141 taacgtaaag gatggtttta atgacagatg cagaaagaga atatcttatt agttacctga
    40201 ttggttattt attgggtcga cgcctggaag ccctggatat cgccaaaaga atgctgtaat
    40261 ccggagtccc tcttgcagac atcatgcgct ttaccggggt gtcagaagaa gagttggctg
    40321 cggcgagcca gtaaagttct gtctcgccat ttcaaaagcc acctacaccc tctgcttcaa
    40381 cgccaccagc atgcgacaaa actcggccgg atgcgaaata aacggcgcat gggccgcttt
    40441 ggcgaagatg tatgattcgc tgtgaggcca aagtttatcc agcatcggca ccactttgcg
    40501 cggcaccaga ccgtcgagat agccatacaa tcgcaaaaac ggcatggaca cgttttgcag
    40561 cggctgacgg agatcgacca ttttcaggat ttccagcccg ccattaagca cgtcaacctc
    40621 cggcatcggt aacgccagaa cggttttctt cagcgcccgc gcatcctggc gcgccgtttc
    40681 agtacccatg gtttgtaacg ccaggaaccg ctccaccgta cgctgaaaat catcactgag
    40741 ttgctgctga aatcccgcca gcacgtccgg ttttatcccc ggccactcgt cacgagcact
    40801 aaaacaaggt gacgacgcca cggtgaccag cgcctgaaca cgctcgggat gggttaacgc
    40861 aatctggctt gccaccagcc cgcccagact ccagcctaac caaatggctt tatcaggtgc
    40921 ctgtcgcagc acggcttcgg ccatatcagc aagtgacatc gcaccaaatc cccggctacg
    40981 cccgaagccg ggcagatcaa caaggtgcag cgtaaaatgc gagctcagtt cctcgtcaat
    41041 gcaacgccac acttcggcat tcagtcccca tccgtgcagc agcacaagat gaacattccc
    41101 ctgacctttg gtctgccacc agatgttatt catccgctat tgttctcttt tgacttacaa
    41161 ggatgaacat atgctaacag taccgggatt atgctggcta tgccgaatgc cgctggcgtt
    41221 aggtcattgg gggatttgtt cggtatgctc acgcgccgcc cgtacagata aaacgttatg
    41281 cccccaatgt ggattacccg ccacacactc acatcttccc tgcggtcgct gcctgcaaaa
    41341 accgccgccc tggcaaagac tggtcacggt tgccgactat gcgccgccgt taagtccgct
    41401 tatccaccag cttaagtttt cccggcgcag cgaaatcgcc agcgccctgg cacgtctgtt
    41461 actattggaa gtcttacacg ctcgtcgcac caccgggttg caattgccgg atcgcattat
    41521 cagcgttccg ttatggcagc agcgtcactg gcgtcgggga tttaatcaga gcgatttgct
    41581 gtgtcagccg ttatcacgct ggttgcactg ccgatgggat agcgaagccg tcacacgaac
    41641 acgggccact gcgacccagc attttctcag tgcccggctg cgcaagcgca acctgaaaaa
    41701 tgcctttcgc cttgaattgc ctgtgcaagg tcgccatatg gtgattgtgg atgatgtcgt
    41761 caccaccgga agtaccgtcg cagagattgc gcagttgctt ttacgcaatg gtgcggcgac
    41821 tgtccaggtc tggtgccttt gtcgaacctt gtagagcctc gatgatgggc gtattataac
    41881 caactaaaat agtcaactat taggccatta ctatgatccg tatttccgat gctgcacaag
    41941 cgcactttgc caaactgctg gcaaatcagg aagaagggac acaaatccgc gtatttgtga
    42001 ttaaccctgg cacgcctaac gctgaatgtg gcgtttctta ttgtccgccg gacgctgtag
    42061 aagccaccga cactgccctg aaatttgacc tgctgaccgc gtatgttgat gagttaagcg
    42121 ctccttacct ggaagatgca gagatcgact ttgttacaga ccagttgggt tcccagttaa
    42181 cgctgaaagc gcctaacgcc aaaatgcgta aagtcgcaga cgatgcaccg ctgatggagc
    42241 gcgtggagta tatgctgcag tcgcagatca acccacagct tgctggtcac ggtggtcgcg
    42301 tttcgctgat ggaaatcacc gaagacggtt acgccattct gcaatttggc ggcggctgta
    42361 acggttgttc tatggtagat gtgacgctga aagaagggat cgagaagcag ctgctgaacg
    42421 aattcccgga gctgaaaggt gtgcgcgatc tcaccgaaca ccagcgcggc gagcactcct
    42481 actactaagt tatcccctct tttgtagatt gcccgatgcg acgctaaagc gtcatttcgg
    42541 gcctacgttg aacatgtgcc agacgttggc gcagcattgc tttggcaaac ccctcccttc
    42601 gtttccccgc gataatatga ccaacctctc ataatttaaa tttaccccgc tctggtgatt
    42661 ctcaaacgcc ggatgttacc cgtatcattc acgtgggtac caaacatact cctgacatct
    42721 gactacaata attagtttta gtgggtatca gtcgtggtgc cgcaatatct ctgttcccga
    42781 ttgggataat tagagtttgt cgtcagaaaa ttgacgttac ccataacaaa tgaaaggcca
    42841 ggtaaatcat gccattagtc attgttgcta tcggtgtaat cttgttgttg ctcctgatga
    42901 tccgtttcaa aatgaacggc ttcatcgctc tcgtcctcgt ggcgcttgct gttggattaa
    42961 tgcaaggaat gccgctggat aaagttattg gctccatcaa agcaggtgtc ggcggaacgc
    43021 tcggtagcct tgccctgatc atgggttttg gcgcaatgct gggcaaaatg ctggcagact
    43081 gcggtggcgc acaacgtatc gccaccacgc tgattgctaa atttggtaaa aaacacatcc
    43141 agtgggcggt ggtactgacc ggttttaccg ttggttttgc cctgttctat gaagtgggct
    43201 ttgtgctgat gctgccgctg gtgtttacca tcgcggcttc cgcgaatatt ccactgctgt
    43261 atgttggtgt accaatggcg gctgcactgt ctgtgaccca cggcttcctg ccaccgcatc
    43321 cgggcccgac tgcgattgcc accattttca atgccgatat gggtaaaacc ctgctgtacg
    43381 gtactattct ggcaatcccg accgtgattc tcgccggtcc ggtttacgct cgcgtgctga
    43441 aaggtataga taagccgatc ccggaaggtc tgtacagcgc gaaaaccttc agcgaagaag
    43501 agatgccaag ttttggcgtc agcgtctgga cctctctggt cccggttgtg ctgatggcga
    43561 tgcgtgcgat tgccgaaatg atcctgccga aaggtcacgc tttcctgccg gtagcggagt
    43621 tcctcggtga cccggtaatg gctacgctga ttgccgtact gattgcgatg ttcacctttg
    43681 gtctgaaccg tggtcgttca atggatcaga ttaacgacac gctggtttct tccatcaaaa
    43741 tcattgcgat gatgctgttg attatcggtg gtggcggtgc gttcaagcag gtgctggtag
    43801 acagcggcgt ggacaaatac attgcttcca tgatgcacga aaccaacatt tctccgctgc
    43861 tgatggcctg gtcgattgct gccgtactgc gtatcgcgct gggttccgca accgttgcgg
    43921 caatcactgc gggtggtatc gcggcaccgc tgattgcaac gacgggtgtt agcccggagc
    43981 tgatggttat tgcggttggt tccggtagtg tgattttctc tcacgtgaac gatccgggct
    44041 tctggctgtt caaagagtac tttaacctga ctatcggcga gaccatcaaa tcctggtcga
    44101 tgctggaaac cattatctcg gtgtgcggtc tggtaggctg tctgctgctg aatatggtga
    44161 tttgataaca ctgactgccg gatgcatcgc atccggcatc aaccgcactc tacttcttct
    44221 tcgctgccgc tctgcgccgt ctgtccaaat ccttcagcaa cttgttcaca ccatcatcgg
    44281 caaacatcgc ctcaagcgtt gcggagagct tgcgccgcca gtttttatac tggtaactgg
    44341 tgccgggaat atttaccggg tccgccatat ccagccagtc ttctggctgt agtcctaaca
    44401 gggcgctgtt gctatcggcg atgtagcgct gtaagccacg attcagcgtc ggtgtcatcg
    44461 acatcaacga tgccttatgc ccggcacgtt ttggcaggca accatattta tgcagagcat
    44521 ccagtagtcc ttgcttcgcc agttcgcgat cctgatacag accgcgcagt accacttcat
    44581 ccggatacag ccccagcgtt ttgcccagcg ttaaatcccc gctctcccag tagccgcgta
    44641 gcgtaggcag gtcgtgtgtc gccgcaaccg ccatcgactg ctctgggtac gctttcggtg
    44701 cgcggaatgt cttctcgtgg tcgttttcga aatagagcac tttgtaggag tacacaccgc
    44761 tgctgcgcag cttaccgaca atctctaccg gtacggtacc gagatcttca ccaatcacca
    44821 tacagcgatg acgtttactt tcgagtgcca gaatcgagag cagatcatcc accggatagt
    44881 gaacatacgc gccctgatct gccgtctcgc catacggtat ccaccacaaa cgcagcatcg
    44941 acatcacgtg atcaattcgt aatgcgccgc agttttgcat attggcacgc aacagctcga
    45001 taaacggttc ataggcacgc gcggtgataa tatgcgggtc cattggcggc aatccccagt
    45061 tctgccccaa cgggccgagg atatccggcg gtgctccaac cgacgctttc aggcaataca
    45121 actcccggtc gcaccaggtt tctgatccac cctctgctac accaaccgcc agatcacgat
    45181 acaagccaat cggcatttca tagccctggc ttatctccca gcaggcggca aactggctgt
    45241 aagccagcca ctgcaaccag agataaaaat cgacgtcatc acgatgttct tcgcagaact
    45301 ggcgcacttc tggtgaatcc acgttctgat acatctctgg ccatgcaggc cagccccagc
    45361 gcatttcgtc ctctttcact tgctgggcat gtagcgcgtc aaacgccgcc tgccagaaca
    45421 ggctatcgcc ttgctcggca acaaactgac gaaacgcggc cacctgttca tcatcacgtt
    45481 gcgcgaaacc tttccacgcc atgcgcaatg ctgtcatttt tagggcggta accgtggagt
    45541 aatcgaccca gtcggcatcc cgcgtctgtt gcagcgtctg ttgcgtggtc ggcaacttcc
    45601 accaggcctg agcctcttcg ctaagatgga aatcttcgac ggcgttaacg tcgatataaa
    45661 tcacattcag ccaacggcga gaagacgggc tgtatgggct ggcactttcc ggatttgccg
    45721 gatagagcgc atgaatcggg ttcagaccga tgaacgaccc gccacgtttt gccacatcca
    45781 ccagcatcgc tttgagatcg ccaaaatccc caatacccca gtttttttcc gatcgcagcg
    45841 tataaagctg aacgcaggca ccccacagct tttgtttatt cagcaacgcc tgcggttcgt
    45901 aacagcgttt cggggcgaca atcaccctgc aatgcacgcg ctggtcttcc tgggtaaacg
    45961 tcagcgtgtg ataaccttcc ggcagcttcg tcggtagatt gaacgctttg ccccccgtta
    46021 catggccttt gtactgcgtt ccttcttcgg tggtcagcag ccagctatat tcgccgctgc
    46081 cctccaccac catcggcatt tttttgccgc tggtataaac catgacattc gggactggcg
    46141 ttaccgccac tttcgtggcg gtacgttgat gcatcgcgtc aagcaaacgc cgtttggttt
    46201 cggcgctaat cgactgcggt ttaccgtggg cattgatgta attggggcta atccccgccg
    46261 ccagcgcggc attatccaga cgtttgcttt ccatcgagct tccttagcgt tttgcctgcc
    46321 agatacgagc ctgataatcg cgaatagagc gatccgagct aaacataccg cagcgggcgg
    46381 tattgaggat cgccgcgcga gtccaggcct cctggtcgcg gtacagcaca tccacctgct
    46441 tttgtgcctc tacataggct gcgaaatccg ccatcaccag atacggatcg ccgccctgtt
    46501 tgccgatact gtgcagcatc tgatcgaagg catgcttatc gccgtcgctg tatttgccgc
    46561 tttccagctc tttcaatact gcgtccagca ccttatcttt cttccgccat ttcaccgggt
    46621 cgtagccttt ggccagaatt gccttcactt gttccacggt atgaccaaaa ataaagatat
    46681 tttcttcacc aactttctcg gcgatttcaa cgttcgcacc atccagcgta ccgacagtaa
    46741 gcgcaccatt gagcgccagt ttcatattgc cggtaccgga agcttcttta cctgccgtcg
    46801 aaatttgttc ggagatatcc gccgccggga tcagtttttc cgccgccgaa acgcaataat
    46861 ccggcaggaa caccaccttc aatttgtcgc caacctgcgg atcgttgttg atcacgtcag
    46921 ccactttgtt gatcgcaaag ataatgtttt tcgccaggta gtagcccggt gccgctttcg
    46981 cgccaaagag gaagacgcgc ggtacgcgat cagcctgcgg gttttcacga atttctttgt
    47041 acaacgccag aatatgcagc agattcaggt gctggcgttt gtactcatgc agacgtttga
    47101 tctgaatatc gaaaatcgcc tgtggattga tctcaatacc ggtacgaact ttcacaaact
    47161 ctgccagacg gactttattc gcctgcttga tctcgcgata ttgctgacgg aatttcgcat
    47221 catcagcgaa tttttccaga ttgatcagtt gatcgagatc gttagtccac tcttttttca
    47281 gtgatttatc caacagagcc gccagtgccg ggttgcactg tttgatccag cgacgtgggg
    47341 taataccgtt ggtgacgtta tggaatttgt tcggccatag ctggtgatat tccgggaaca
    47401 gatctttcac caccagatcc gagtgcaggg ccgcaacacc gtttaccgcg aaaccgccaa
    47461 ccacacacag gttcgccata tgcacttgtt tgtcgtgtac caccgccagt ttggcccata
    47521 ctttttcatc gcccggccag gttttttcca ccagcgtttt aaagcgtgta ttaatttcgt
    47581 taataatctg catgtggcgc ggcagtaagc ctttcaccag tttcacatcc cagcgttcca
    47641 gcgcttctgg catcaaggta tggttggtgt aagcgaaagt tttactggtg atggcccagg
    47701 catcatccca gctcatctgg tgctcatcga tcagcacgcg cagcagttct ggaatcgcga
    47761 tagttgggtg ggtatcgttc agctgaataa cttcgtactc cgccagctcg tgcagtttac
    47821 gccccgccag atgatggcga cgcaaaatat ccgctaccga gcaggcacac tggaagtatt
    47881 gctgcatcag gcgcagcttt ttaccggcag tatggttgtc gtttggatag agaactttgg
    47941 tcagtttttc cgcgttgatg ccctgctgtt cggcacgcag gaaatcacca tcgttaaatt
    48001 tagtcagatc aaacggatgc gcatgcgtcg cctgccacag acgcagcggc tgcgccacgc
    48061 cattacgata gccgacaacg gggagatccc acgcttgacc ggtaatggta aactccggct
    48121 cccagcgtcc gtctttcgtc actttaccgc caatccctac ctgcacatcc agtgcttcgt
    48181 tgtggcggaa ccacgggtag ttactgcgat gccagtcatc cggcgcttca acctgtttgc
    48241 catcgacaaa tgactggcgg aacaagccat attgataatt aaggccgtaa ccagtagctg
    48301 actgcccgac agttgccatt gagtcgagga agcacgccgc cagacgtccc agaccaccgt
    48361 tacccagcgc cgggtcgatc tcttcttcca gcaagtcagt caggttgatg tcataagcct
    48421 tcaacgaatc ctgtacatcc tgataccagc cgagattcaa cagattgttg cccgtcaggc
    48481 gaccaatcaa aaactccatt gagatgtagt taacatgccg ctgattcgcc accggtttgg
    48541 caaatggctg agcacgcagc atttcggcca gtgcttcgct cactgccaac caccactgac
    48601 gaggggtcat ttcagccgca gaatttaagc cataacgctg ccactgacgt gaaagcgctt
    48661 cctgaaattg cttatcgtta aaaataggtt gtgacatagg agttccactt ttcttagatt
    48721 ttcaacacaa cgttatcgct agtttgccag gctcgatgtt gaccttcctc atcctgcggg
    48781 ggattaggca gggaggagtt gcggggatga gcaaggaaat gtgatctcga ccacttaaag
    48841 ctagtgcaaa ccacaggatt agcagcaaat caatgccata cagcgcagaa aatctgtatc
    48901 aaagtgcaaa aaatggccgt tgcgtatttt caaaaagcgg aaggtaactc tataattaag
    48961 taaaggagtg aaacagtttc acaagtaaaa tatccagtgt gctccatctc attcttaata
    49021 gatttattaa gatcatcttt ttagatggca ctttcatcag aaatgaagaa gaaacccttg
    49081 cttaaatgaa actgatgaac ataaggaaaa taccgtacaa cccagtattc acgctggatc
    49141 agcgtcgttt taggtgagtt gttaataaac atttggaatt gtgacacagt gcaaattcag
    49201 acacataaaa aaacgtcatc gcttgcatta gaaaggtttc tggccgacct tataaccatt
    49261 aattacgaag cgcaaaaaaa ataatatttc ctcattttcc acagtgaagt gattaactat
    49321 gctgattccg tcaaaattaa gtcgcccggt tcgactcgac cataccgtgg ttcgtgagcg
    49381 cctgctggct aaactttccg gcgcgaacaa cttccggctg gcgctgatca caagtcctgc
    49441 gggctacgga aagaccacgc tcatttccca gtgggcggca ggcaaaaacg atatcggctg
    49501 gtactcgctg gatgaaggtg ataaccagca agagcgtttc gccagctatc tcattgccgc
    49561 cgtgcaacag gcaaccaatg gtcactgcgc gatatgtgag acgatggcgc aaaaacggca
    49621 atatgccagc ctgacgtcac tcttcgccca gcttttcatt gagctggcgg aatggcatag
    49681 cccactttat ctggtcatcg atgactatca tctgatcact aatcctgtga tccacgagtc
    49741 aatgcgcttc tttattcgcc atcaaccaga aaatctcacc cttgtggtgt tgtcacgcaa
    49801 ccttccgcaa ctgggcattg ccaatctgcg tgttcgtgac cagctgctgg aaattggcag
    49861 tcagcaactg gcatttaccc atcaggaagc gaagcagttt tttgattgcc gtctgtcatc
    49921 gccaattgaa gccgcagaaa gcagtcggat ttgcgatgac gtttccggtt gggcgacggc
    49981 actgcagcta atcgccctct ccgcccggca gaatactcac tcagcccata agtcggcacg
    50041 ccgcctggcg ggaatcaatg ccagccatct ttcggattat ctggtcgatg aggttttgga
    50101 taacgtcgat ctcgcaacgc gccattttct gttgaaaagc gccattttgc gctcaatgaa
    50161 cgatgcactc atcacccgtg tgaccggcga agaaaacggg caaatgcgcc tcgaagagat
    50221 tgagcgtcag gggctgtttt tacagcggat ggatgatacc ggcgattggt tttgctatca
    50281 cccactgttt ggtaacttcc tgcgccagcg ctgccagtgg gaactggcgg cggagctgcc
    50341 ggaaatccac cgtgccgccg cagaaagctg gatggcccag ggatttccca gcgaagcgat
    50401 tcatcatgcg ctggcggcag gcgatgcgct gatgctgcgc gatattctgc ttaatcacgc
    50461 ctggagtttg ttcaaccata gcgaactgtc gctgctggaa gagtcgctta aggccctgcc
    50521 gtgggacagt ttgctggaaa atccgcagtt ggtgttattg caggcgtggc tgatgcaaag
    50581 ccagcatcgc tacggcgaag ttaacaccct actggcccgc gctgaacatg aaatcaagga
    50641 cattagagaa ggcaccatgc acgcagaatt taacgctctg cgcgcccagg tagcgattaa
    50701 cgatggtaat ccggatgaag cggaacggct ggcaaaactg gcactggaag agctgccgcc
    50761 gggctggttc tatagccgca ttgtggcaac ctcggtgctg ggtgaagtgc tgcactgcaa
    50821 aggcgaattg acccgctcac tggcgctaat gcagcaaacc gaacagatgg cacgccagca
    50881 cgacgtctgg cactacgcct tatggagttt aatccagcaa agtgaaattc tgtttgctca
    50941 gggattcctg caaaccgcgt gggaaacgca ggaaaaagcg ttccagctga tcaacgagca
    51001 gcatctggaa cagctgccaa tgcatgagtt tctggtgcgc atccgagcac aactgttatg
    51061 ggcctgggca cggctggatg aagccgaagc gtcggcgcgt agcgggattg aagtcttgtc
    51121 gtcttatcag ccacagcaac agcttcagtg cctggcaatg ttgattcaat gctcgctggc
    51181 ccgtggtgat ttagataacg ctcgtagcca gcttaaccgt ctggaaaacc tgctggggaa
    51241 tggcaaatat cacagcgact ggatctctaa cgccaacaaa gtccgggtga tttactggca
    51301 aatgaccagc gataaagccg ccgctgccaa ctggttgcgt catacggcta aaccggagtt
    51361 tgcgaacaac cacttcctgc aaggtcaatg gcacaacatt gcccgtgcac aaatcttgct
    51421 gggcgagttt gaaccggcag aaattgttct cgaagaactc aatgaaaacg cccgcagtct
    51481 gcggttgatg agcgatctca accgtaacct gttgctgctt aatcaactgt actggcaggc
    51541 cggacgtaaa agtgacgccc agcgtgtgtt gctggacgca ttaaaactgg cgaatcgcac
    51601 cggatttatc agtcattttg tcatcgaagg cgaagcgatg gcgcaacaac tgcgccagct
    51661 tattcaactc aatacgctgc cggagctgga acagcatcgc gcacagcgta ttctgcgaga
    51721 aatcaatcaa catcatcggc ataaattcgc ccatttcgat gagaatttcg ttgaacgtct
    51781 gctaaatcat ccggaagtgc cagaactgat ccgcaccagc ccgttgaccc aacgtgaatg
    51841 gcaggtcctg gggctgattt actccggtta cagcaacgag caaattgcag gtgagctgga
    51901 agtggcggca accaccatca agacgcatat ccgcaatctg taccaaaaac tcggtgtcgc
    51961 ccaccgtcag gccgcggtac aacacgcgca gaaactgctg aagatgatgg gatatggggt
    52021 gtgagtttag ccggataata cgtcggatcc ggcttacatc tttatatcat tcaatgctca
    52081 cccgcgttac gccatccgcc tccaccagac taaaccgcac tggcaagaaa cgctccacca
    52141 ccgcaatatt ggtcagcaga tggcatgagg gatgggcgac cgtaaattct cccgcgcccg
    52201 ccagcgccat cggcagtacc agctggtcgg cgagatattc ccccaccgcc gccggacttg
    52261 ccaggtagcg tttcacctct ttcaccaact gtgccgcgac cacttcggcg ctgacgcgct
    52321 tttcaccgac gacaaaaaag cgttcggtga tattttcact ttcgacttca agcgaaacgg
    52381 tattacccgg tccctggtca cgcggcaggt tatgaatatt ctgctcatgc agggaaaaac
    52441 tccccgccag tgtagcgatt tcacgctcag cgacatggcg cggcacgcca gctaatagca
    52501 cctctccacg catctgcaca atgttcccgc gctcgccaag ttgcaaggtg ttaaacaatg
    52561 ccaccggcga gacttccgtt gccaccaccc cgcctccagc aggataaaaa ccgtggcgta
    52621 ataatgtggt ttgctgatga atgcccattt tcgccagcag cggctccagc acccggcgga
    52681 taaaatccgc aggcggtgcc gacgggttat cggtgccgcc gctcacttca acacgcgaag
    52741 gcccatcagc aaaccacagc gcgggtagca ccgtttgcag caccagcgtg ctgcttccgg
    52801 cactaccgat agcaaagcgg taatcgccgc cacgcacggt accgggccgg aagaccagac
    52861 gctgtgaccc cagctccgcg ccttccaccg ttgccctaca aatttccgca gccgctttta
    52921 ccgcggtcag atgttggcgc aacagccccg gtttcgcccg cccggcacga atgccggtga
    52981 tggtaaatgg ctggccggtt atcatcgaca ggctcagcgc cgagcgcagg atctgcccgc
    53041 cgccttcgcc ctgtgcgcca tccagcgcaa tcatcctttt catcatttat ccttttacgc
    53101 acaccacctg acgcagggta tagataactt ccaccagatc gctttgtgcc gccatcaccg
    53161 catcaatatc tttatacgcc atcgggattt cgtcgatcac ttcggcatct ttacggcatt
    53221 ccacatgcgc ggtggcacga atttgatctt ccacgctgaa catttttttc gctttagtac
    53281 ggctcataac ccgcccggca ccgtggctgc acgaacagaa cgactcttca tttcccagcc
    53341 cacggacgat aaagcttttc gctcccatcg accctggaat aattccatat tgaccagcac
    53401 gcgcagacac cgcgccttta cgcgtcacgt agatctcttc accaaagtgc tgttcttttt
    53461 gcacatagtt gtggtgacag ttgatctctt ccatcgccag cgtttgtggc tgtctgaccg
    53521 ttttctgcgt aatgctctgc aacgccgtta ccacgttttc catcatcgca tcgcggttaa
    53581 ggctggcaaa aagctgcgcc caggccacgg ctttcaggta atcatcaaag tattccgtac
    53641 cttccataaa gtacgccaga tcacgcgacg gcaatgtttc aagcgtttcc tgcatctctt
    53701 tttgcgccag atcgataaag taagtcccga tggcgttacc aattccgcgt gaaccggagt
    53761 gcagcataat ccacacctgg tccgactcat caaggcagat ttcaataaag tggttacccg
    53821 ttcccagcgt tcccaggtgt ttatagttgt tagtgttaag gaaacgggga tatttttgcg
    53881 ttaaccactg ataaccggct tccagctcag cccatttcgc atcgacgtta acaggcggat
    53941 tttcccatgc gcctttatca cgtttacaac gtccggtagt acgtccgtgc ggcacggctg
    54001 tttcaatcgc ctgacgcagc tccgccaggt tttccggcag gtctgccgcc gttaacgcgg
    54061 tacgcagcgc gttcattcca caaccgatat ccacgcccac cgccgccgga ataatcgccc
    54121 ctttggtcgg gatcacgcta ccaatggtgg aacctttacc caggtgtaca tccggcatta
    54181 ccgcaatatg tttgaaaata aacggcatct tcgccgtatt aataagttgc tgacgcgcat
    54241 cggcctctac cggcacgcct ttggtccaca tttttaccgg ggcattttca gtggtcagta
    54301 attcgtaatt cattttgttt ttctcttttc gttgtttgct gtcctgataa ttgcaaccgt
    54361 cgtgccagaa aattaaataa gcagcttaat ttttttattt attgttttta aaaagattat
    54421 atctttacgt ccgtaaccgg agatttcccg caaagccgat ttaccgataa tgaaatctcg
    54481 tcttttataa ggatatctaa gatgcgtaaa acagtggctt ttggctttgt cggaacggtg
    54541 ctggattatg ccgggcgcgg cagtcagcgc tggtcgaaat ggcgtccgtc actctgtcta
    54601 tgccagcaag aatcgttagt catcaatcga ctggaattgt tgcacgacgc ccgctcgcgc
    54661 tcgctgtttg aaacgcttaa atgcgatatc gccagcgttt cgccagaaac tgaagtggtg
    54721 agcgttgaga ttgaactgca taacccgtgg gatttcgaag aggtttacgc ctgcctgcat
    54781 gatttcgccc gtggctacgc gtttcaacct gataaagaag actatttaat tcacatcacc
    54841 actggcaccc acgtcgcaca gatttgctgg tttctgctgg cggaagcgcg ttacctgccc
    54901 gcccggctga tacaatcttc cccaccgcgc aaaaaagaac agccgcgcgg cgcaggtgaa
    54961 gtgacgatta tcgatctcga tttaagccgt tataacgcca tcgccagccg ctttgccgag
    55021 gaacggcagc aaacgctcga ttttcttaag tccggcattg ccacgcgtaa cccccacttc
    55081 aaccgcatga ttgagcagat tgaaaaagtg gcgattaaat cccgcgcacc gatcctgctt
    55141 aacggcccga caggtgcggg caagtcattt ctggcacgac gcatcttcga attaaaacag
    55201 gcacggcatc agtttagcgg cgcatttgta gaaatgaact gcgccactct gcgtggcgat
    55261 accgccatgt cgacgctatt tggtcatgtg aaaggcgcgt ttaccggggc gcgggagtcg
    55321 cgggaaggat tattacgcag cgccaacggc gggatgttat ttcttgatga gattggcgaa
    55381 ctgggcgcag acgaacaggc aatgctgctg aaagccattg aagagaaaac cttttatccg
    55441 tttggcagcg atcgccaggt gagtagcgac tttcagctta tcgccggaac ggtgcgcgat
    55501 ttgcgtcagc tggttgccga aggcaaattc cgcgaagatc tgtacgcgcg gatcaatctc
    55561 tggaccttca ccttgccggg gctgagccag cgccaggaag atattgaacc gaaccttgat
    55621 tatgaagtgg agcgccacgc ctcactcact ggcgacagcg tgcgttttaa caccgaagcg
    55681 cggcgcgcct ggctggcttt tgcgacctct ccccaggcaa catggcgcgg taactttcgc
    55741 gaactttctg ccagcgtcac gcggatggca acctttgcca ctagcggacg catcactcgg
    55801 gaagtggtgg aagatgagat aaaccgtctg cgctataact ggcaggagag tcgcccctcc
    55861 gcgattacgg cgttgctggg cgcagaggca gaaaacatcg atctcttcga ccgtatgcaa
    55921 ctggaacacg taatagccat ctgccgccag gcaaagtcac tttctgctgc cggtcgccag
    55981 ctttttgatg tttcgcgcca gggcaaagcc agcgtcaacg acgcagatcg gctacgcaaa
    56041 tacctggcgc gttttggtct gacgtgggaa gccgtgcagg atcagcacag ctccagttga
    56101 atatgatggt ccgtcagcac ctgcatgacg cttactggcg gcggggcgtc ggtgtagaca
    56161 gcatcaacca tgctgatgct ccccatattg accatcgcat tacggccaaa tttcgagtga
    56221 tcgacaacca gcataacgtg gcgcgagttc tcaataatgg cgcgtttggt gcgaacttcg
    56281 tggtaatcga attccagcaa cgagccgtcg ctatcgatgc cgctgatccc cagaatgccg
    56341 aaatcaaggc ggaactggga gataaaatcg agcgtcgctt cgcctatgat cccaccatcg
    56401 cggctacgta attcgccacc ggcgagaata atgcgaaaat cctcttttac catcagcgtg
    56461 ttagcgacgt taagattgtt ggtgacaatg cgcaaattgc tgtgattgag cagcgcgtgc
    56521 gccaccgctt ccggggtggt gccgatatcg ataaacagcg ttgagccatt aggaatttgc
    56581 tccgccactt tgcgggcgat gcgctctttt tcttcggtct gggtggcttt acgatcgtgc
    56641 catggcgtgt taaccgaact ggaaggcagc gccgcaccgc catgatggcg caggatcagg
    56701 ttttgctctg ccagctcgtt gaggtcgcga cgaatagtct gcgggctgac ggagaagtgc
    56761 tccaccagtt cctcggtact gacataaccc tgctgtttaa ccagttcgat aataccgttg
    56821 tgacgttgtg tttgtttcat ttataaatcc ctggaattat tttcgttttc gcgcattgag
    56881 cgaatcaaca aaagccatcg ctaaacccac ggctaacccg gcgatgtgtg ctccgttcgc
    56941 catcgacatc ccaaacaaat caaaccatcc ggcgacaatc cagatcagcg caaagataat
    57001 taacccacgt tgcaggtaaa tgccactttg cggatcgcgt tcgccacgta gccagacgta
    57061 gcccatcagc gcatacacca cgccagaaag cccgccaaac cacggcccgc tgaatttttg
    57121 ctgcacatag ccgcttaaca gggcgctaat gagagtaatg acaattagct taccgctacc
    57181 gaggcgtttt tccaccgcac cgccgagata ccaccaccag agcaggttaa agaggatatg
    57241 catcagcgag aagtgcatta acgcgtgggt gaagtaacgc cagaactcaa atttcagtgt
    57301 tggatcgaat ggccaggcca gccataacat cacttcctga tcgccgagaa tttgcatggc
    57361 aataaacacc accacgcagg cgatcatcat cacccaggtt accggacctg cgcgttcacg
    57421 caaggcagca aagaaaggat aacggcgata atgcaggcca ctgccggtat ggcccgcctg
    57481 ccagctggcc gccagataac gcggatctgc cgggttttcg agaaaacgcg ccagctctgc
    57541 ccgcacgcgc tcggcctggg actcatccgc cagccagaca tcgctttggt tatgttgttg
    57601 aatcgtgagg ataacaccct gcgtcgccat gtaatcaaca aacgcctgcg ccacgcgggg
    57661 gttagcaaaa gaggtaatca tcaacatcgt tgctgtcgct tattccacac aaaaggggac
    57721 agtataaagc gttacgcgcc gtacgccacc tctgcaggaa actgacgccg ccaggcttca
    57781 aagccgccgt caatgctata gaccacatcg tagccctgtt gcagcagata ctgcgccgcg
    57841 cctttgctgc tattgccgtg ataacacatc accatcaccg gtgtgtcaaa gtcgttatca
    57901 cgcataaacg cgcccagtgt gtcgttagtt aaatggaaag cctgcaccgc atgccccatt
    57961 gcgaaactct gtggatcgcg aatatcgacc agcaccgcct ctttttcctg caacttctgg
    58021 tgcgcgacgg caacgttaat acattcgaac tgctccatgc gtctctcttt ctttacaaac
    58081 aagtgggcaa atttaccgca cagtttacgt cgaagcggca gataaacgcc ataatgttat
    58141 acatatcact ctaaaatgtt ttttcaatgt tacctaaagc gcgattcttt gctaatatgt
    58201 tcgataacga acatttatga gctttaacga aagtgaatga gggcagcatg gaaaccaaag
    58261 atctgattgt gataggtggc ggcatcaatg gtgctggtat cgcggcagac gccgctggac
    58321 gcggtttatc cgtgctgatg ctggaggcgc aggatctcgc ttgcgcgacc tcttccgcca
    58381 gttcaaaact cattcacggt ggcctgcgct accttgagca ctatgaattc cgcctggtca
    58441 gcgaggcgct ggctgaacgt gaagtgctac tgaaaatggc cccgcatatc gccttcccga
    58501 tgcgttttcg cctgccgcat cgtccgcatc tgcgcccggc gtggatgatt cgcattggtc
    58561 tgtttatgta cgatcatctg ggtaaacgca ccagcctgcc gggatcaacc ggtttgcgtt
    58621 ttggcgcaaa ttcagtgtta aaaccggaaa ttaagcgcgg attcgaatat tccgactgtt
    58681 gggtagacga cgcccgtctg gtactcgcca acgcccagat ggtagtgcgt aaaggcggtg
    58741 aagtgcttac tcgtactcgt gccacttctg ctcgccgcga aaacggcctg tggattgtgg
    58801 aagcggaaga tatcgatacc ggcaaaaaat atagctggca agcgcgcggc ctcgtgaacg
    58861 ccaccggccc gtgggtaaag cagttcttcg acgacgggat gcatctgcct tcgccttatg
    58921 gcattcgcct gatcaaaggc agccatattg tcgtgccgcg cgttcataca caaaagcagg
    58981 cctacattct gcaaaacgaa gataaacgta ttgtcttcgt gatcccgtgg atggacgagt
    59041 tttccatcat cggcaccacc gatgtggaat acaaaggtga tccaaaagca gtgaagattg
    59101 aagagagtga aatcaattac ctgctgaaag tgtataacac gcactttaaa aagcagttaa
    59161 gccgggacga tatcgtctgg acttactccg gcgtgcgtcc gctgtgtgat gatgagtccg
    59221 attcgccgca ggctatcact cgtgattaca cccttgatat ccatgatgaa aacggcaaag
    59281 cgccgctgct gtcggtattc ggcggtaagc tgaccaccta ccgcaaactg gcggagcacg
    59341 cgctggaaaa actgacgcca tattatcagg gcattggtcc ggcgtggacg aaagagagcg
    59401 tgctgccggg tggtgccatt gaaggcgacc gcgacgatta tgccgctcgc ctgcgccgcc
    59461 gctatccgtt cctgactgaa tcgctggcac gccattacgc gcgcacgtac ggcagcaaca
    59521 gcgagttgct gctcggcaat gcggggacgg taagcgatct cggggaagat ttcggtcatg
    59581 agttctacga agcggagctg aaatacctgg tggatcacga atgggtccgc cgcgccgacg
    59641 atgccctgtg gcgtcgcaca aaacaaggca tgtggctgaa tgcggaacaa caatctcgtg
    59701 tgagtcagtg gctggtggag tatacgcagc agaagttgtc gctggcgtcg taaggatgat
    59761 gcccggttgt gaagtgcaac cgggctattt ggattcctta gactattcat tgtaaataat
    59821 tgaataacgg cccctgagct aaaatcgcca aacttaagca cgttccccac tcaacccact
    59881 tctgcccctg gcttctattt ttgctacgcc actggcagta atatcccaac gcaaaaccaa
    59941 gctgtgcact aatcgggccg acaaaatcag tccacggctg cggccaaaaa ggggaaaaat
    60001 tcaggggaag ccagacaaac gtcagaaaga agtttcggat attttccagc aacgctgatt
    60061 tctctggagt aatataaaga atgaaattcg agagaattaa ccagaaatag gccggagaaa
    60121 agaccagcca gtaatgcagt ctgtttttta attcaggagg cgttctgcgg gagaattgcc
    60181 aaccgatgcc acccattaca ctgaaaaaaa gtgcatattc aaccattaac tcaagttgta
    60241 attcctgaag agaaatacgc acaaccagga gcggaaagga gaatattatt tcagccagaa
    60301 aagttaacgt gaatataagt acggctaagg caataaccgt cggccatacc aataatggca
    60361 cgataacaaa accgagaatt acagggggaa tataaagccg gaatttaatc atttttattt
    60421 tcctggtgat tcggatgatg cgtcatacat cgcgcataaa cactcatcaa tcatccagtc
    60481 gtcttcccag acctgataat gtgtgccctg ttcgggctga ttaagcaggt tctcaatcgc
    60541 ctgccgcgca cgttctttca ggatgaattg attgttgaag gcccaggcat tgatttgggc
    60601 ggtaatggct ttatcgtcat catcctgctc aaaacgacta ttctgaatat tctcttccct
    60661 gttatgccac agcaaacatg ccatcgatac tgtgggttgg taaccgggta cagagatgat
    60721 ggcatcaagc aatggcgaaa gagcgatatc ggcacgggaa aacatttcca gcatatactg
    60781 taattcgaca gcctcatcgc cagataccca atcagccata tactggcagg caaatcgctg
    60841 caatattgcc cgttcatcct cacgccagtt ttctttactg gcaaggttga tacgccgtag
    60901 agaaaatact tcgtgaatat tgtcaaattg cccctgcgca acaaactcca gtaaacgagg
    60961 aagaaaataa cgaacctcat cgctgttttg cggatcaggt cctggactac tattccaggc
    61021 attgattaag gaataaggaa tcgctcgcaa cgatgtattc cgtaatgctt tttgttcctg
    61081 ttgactcagg caatattcac aacaaaccac aaattgctgc ggcaccggat aacgacgaaa
    61141 aatatgataa acctgctcaa ttaatgcctg catggcggag ctgggttgca taattacccc
    61201 ctcttccgct ggtcgcgtgt aaacggttga taaacaaaac gagcattcgc cgcattcagg
    61261 cgaataatgg aactacgaga gagggaaaag ctgggccgaa tgcaataatt acaatcccga
    61321 cagctttcct ctgtgcaatt ttccactgag taatgacgcg cgtagagttt cagttcagcc
    61381 tgcttatccc gaaaaccgcg taagctgtta tcggtatgga atttccatgc ttctttaaac
    61441 acctgaaaga tataaacatc ctcaatccag gagtcacatt ttttaagcgg gcgctttgct
    61501 atgatcaccc gctcatccgg attgagcatt gccgtaaaat gaacggtaaa gtcatttgtc
    61561 gtattcaggt cacattgaac atctggtaca tcactgatat aggggtcaaa tccaaaatca
    61621 gcaccacagg cacaaagacg accgatcgtg gcggtatcga gggaaaatcc cggccataca
    61681 ttgctggagg ttaagccaat atttaatgtg acatcatatc gagataatcc ctcaagaccg
    61741 acagtaccgg cgaacttttc cccttcatcc aggacctggt taatgagttg ctgaataatg
    61801 ccttcatccc agtatgttga tttgcagttg gcaatcgtaa tatccacgcc agtgaatgga
    61861 tcagtaccgg agaggcttgc aaataacgta tcaactaatt ccatttttat tatccggctt
    61921 aatgcaacat tttatccatc ggcacataac ctacattttc caccagattt tgtccctgta
    61981 gaattaaaaa tatttttttg ttttacattc tgttcatccc tattattgca ttttattttg
    62041 cggtgatggc taaaccgatt ctgtactagc tcaaaaatta ctgatattcc actcgcaata
    62101 cagccagtgc attataacga cctgccgcag gcacttttcc agttgtacta acaggtaaag
    62161 ccaggaaagt aacatcagca gtttgcgcag aagcagcctg gttttccacc aatctgaacg
    62221 gaacgcctac tgatgttgcc agagcattta aatctacgga gttttgctga ctatccataa
    62281 tcataatacc aacatcagga ttatcttgtg cctgaataat actttgtttc ccattggtat
    62341 cacgtgtagc tgaaatgtta ccgtcgaata ccaacgtcat taccgcatct gtatctttat
    62401 tcgtacaatt aatcggaata gttgttgcta caggagtaac accagcaggc ttcgctccag
    62461 caccagcccg gctcaattca ctggcccaca catccggcat attaacagta aaactagtac
    62521 cctcattaat agtgcaacct tgcggaacct gaatatccag gttactgatc caggcattaa
    62581 ataatggtac tggcccatgt tccgtttgcc gctgactggc atacaatgaa actaaaagta
    62641 cattattcag agagacatca gagatgattt ttttatccaa cctaatttta atatagcctt
    62701 ctgttcctga agaccagtaa tctgctttac ttctgtcagc aggataatta gtactatagt
    62761 tctcgaatgg tactgtttta tctgtcatc
//
LOCUS       DAZGQL010000016        62676 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.016, whole genome shotgun sequence.
ACCESSION   DAZGQL010000016 DAZGQL010000000
VERSION     DAZGQL010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 62676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62676
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.016"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            141..644
                     /gene="ftnB"
                     /locus_tag="ACHKIR_001188"
     CDS             141..644
                     /gene="ftnB"
                     /locus_tag="ACHKIR_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HFO9988953.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(722..973)
                     /gene="yecJ"
                     /locus_tag="ACHKIR_001189"
     CDS             complement(722..973)
                     /gene="yecJ"
                     /locus_tag="ACHKIR_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 family protein YecJ"
                     /protein_id="HFO9988954.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTNHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(1088..1174)
                     /gene="azuC"
                     /locus_tag="ACHKIR_001190"
     CDS             complement(1088..1174)
                     /gene="azuC"
                     /locus_tag="ACHKIR_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HFO9988955.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            1437..1760
                     /gene="yecR"
                     /locus_tag="ACHKIR_001191"
     CDS             1437..1760
                     /gene="yecR"
                     /locus_tag="ACHKIR_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HFO9988956.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HARTDRYVSRGIADRACQQAGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            1932..2429
                     /gene="ftnA"
                     /locus_tag="ACHKIR_001192"
     CDS             1932..2429
                     /gene="ftnA"
                     /locus_tag="ACHKIR_001192"
                     /EC_number="1.16.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HFO9988957.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(2467..2706)
                     /gene="yecH"
                     /locus_tag="ACHKIR_001193"
     CDS             complement(2467..2706)
                     /gene="yecH"
                     /locus_tag="ACHKIR_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family metal-binding protein"
                     /protein_id="HFO9988958.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            2896..4107
                     /gene="tyrP"
                     /locus_tag="ACHKIR_001194"
     CDS             2896..4107
                     /gene="tyrP"
                     /locus_tag="ACHKIR_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HFO9988959.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     IIGSAIPLVAYIFWQLATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(4158..4823)
                     /gene="yecA"
                     /locus_tag="ACHKIR_001195"
     CDS             complement(4158..4823)
                     /gene="yecA"
                     /locus_tag="ACHKIR_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HFO9988960.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIESAQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALDAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(5019..5105)
                     /locus_tag="ACHKIR_001196"
     tRNA            complement(5019..5105)
                     /locus_tag="ACHKIR_001196"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5069..5071),aa:Leu,seq:taa)
     gene            complement(5118..5191)
                     /locus_tag="ACHKIR_001197"
     tRNA            complement(5118..5191)
                     /locus_tag="ACHKIR_001197"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5157..5159),aa:Cys,seq:gca)
     gene            complement(5246..5321)
                     /locus_tag="ACHKIR_001198"
     tRNA            complement(5246..5321)
                     /locus_tag="ACHKIR_001198"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5286..5288),aa:Gly,seq:gcc)
     gene            complement(5473..6021)
                     /gene="pgsA"
                     /locus_tag="ACHKIR_001199"
     CDS             complement(5473..6021)
                     /gene="pgsA"
                     /locus_tag="ACHKIR_001199"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFO9988961.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(6078..7910)
                     /gene="uvrC"
                     /locus_tag="ACHKIR_001200"
     CDS             complement(6078..7910)
                     /gene="uvrC"
                     /locus_tag="ACHKIR_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFO9988962.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDESKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(7907..8563)
                     /gene="uvrY"
                     /locus_tag="ACHKIR_001201"
     CDS             complement(7907..8563)
                     /gene="uvrY"
                     /locus_tag="ACHKIR_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HFO9988963.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     ANAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            8859..9035
                     /gene="yecU"
                     /locus_tag="ACHKIR_001202"
     CDS             8859..9035
                     /gene="yecU"
                     /locus_tag="ACHKIR_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecU"
                     /protein_id="HFO9988964.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            9022..9246
                     /gene="yecF"
                     /locus_tag="ACHKIR_001203"
     CDS             9022..9246
                     /gene="yecF"
                     /locus_tag="ACHKIR_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein YecF"
                     /protein_id="HFO9988965.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(9313..10035)
                     /gene="sdiA"
                     /locus_tag="ACHKIR_001204"
     CDS             complement(9313..10035)
                     /gene="sdiA"
                     /locus_tag="ACHKIR_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HFO9988966.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMEAAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPEAWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFSE
                     AQPLWEAARAHGLRRGVTQYLMLPNRALGFLSFSRCTAREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILKWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
     gene            complement(10265..11017)
                     /gene="tcyN"
                     /locus_tag="ACHKIR_001205"
     CDS             complement(10265..11017)
                     /gene="tcyN"
                     /locus_tag="ACHKIR_001205"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     TcyN"
                     /protein_id="HFO9988967.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPEQPRTRQFLEKFLLQ"
     gene            complement(11014..11682)
                     /gene="tcyL"
                     /locus_tag="ACHKIR_001206"
     CDS             complement(11014..11682)
                     /gene="tcyL"
                     /locus_tag="ACHKIR_001206"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HFO9988968.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(11697..12683)
                     /gene="dcyD"
                     /locus_tag="ACHKIR_001207"
     CDS             complement(11697..12683)
                     /gene="dcyD"
                     /locus_tag="ACHKIR_001207"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019148 - D-cysteine desulfhydrase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HFO9988969.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKIGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(12788..13588)
                     /gene="tcyJ"
                     /locus_tag="ACHKIR_001208"
     CDS             complement(12788..13588)
                     /gene="tcyJ"
                     /locus_tag="ACHKIR_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015276 - ligand-gated monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO9988970.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTADDLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(13676..14227)
                     /gene="fliZ"
                     /locus_tag="ACHKIR_001209"
     CDS             complement(13676..14227)
                     /gene="fliZ"
                     /locus_tag="ACHKIR_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HFO9988971.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDMLLDENGWQVEQKSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(14273..14992)
                     /gene="fliA"
                     /locus_tag="ACHKIR_001210"
     CDS             complement(14273..14992)
                     /gene="fliA"
                     /locus_tag="ACHKIR_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HFO9988972.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(15311..17050)
                     /locus_tag="ACHKIR_001211"
     CDS             complement(15311..17050)
                     /locus_tag="ACHKIR_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001602758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliC/FljB family flagellin"
                     /protein_id="HFO9988973.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTITIDL
                     KKIDSDTLGLSGFNVNGGGAVANTAATKSDLAAAQLLAPGTADANGTVTYTVSAGLKT
                     STAADVIASLANNAKVNATIANGFGSPTATDYTYNSATGDFTYSATIAAGTNSGDSNS
                     AQLQSFLTPKAGDTANLNVKIGSTSIDVVLASDGKITAKDGSELFIDVDGNLTQNNAG
                     TVKAATLDALTKNWHTTGTPGAVSTVITTEDETTFTLDGGTDATTSGAITVANARMSA
                     ESLQSATKSTGFTVDVGATGTSAGDIKVDSKGIVQQYTGTVFEDAYTKADGSLTTDNT
                     TNLFLQKDGTVTNGSGKAVYVSADGNFTTDAETKAATTADPLKALDEAISSIDKFRSS
                     LGAVQNRLDSAVTNLNNTTTNLSEAQSRIQDADYATEVSNMSKAQIIQQAGNSVLAKA
                     NQVPQQVLSLLQG"
     gene            17316..18722
                     /gene="fliD"
                     /locus_tag="ACHKIR_001212"
     CDS             17316..18722
                     /gene="fliD"
                     /locus_tag="ACHKIR_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0009421 - bacterial-type flagellum
                     filament cap [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFO9988974.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLIIQQGGDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKAQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSNYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            18747..19157
                     /gene="fliS"
                     /locus_tag="ACHKIR_001213"
     CDS             18747..19157
                     /gene="fliS"
                     /locus_tag="ACHKIR_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000270662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HFO9988975.1"
                     /translation="MYAAKGTQAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPD"
     gene            19157..19522
                     /gene="fliT"
                     /locus_tag="ACHKIR_001214"
     CDS             19157..19522
                     /gene="fliT"
                     /locus_tag="ACHKIR_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:1902209 - negative regulation of
                     bacterial-type flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HFO9988976.1"
                     /translation="MNNAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            19600..21087
                     /gene="amyA"
                     /locus_tag="ACHKIR_001215"
     CDS             19600..21087
                     /gene="amyA"
                     /locus_tag="ACHKIR_001215"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFO9988977.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(21121..21534)
                     /gene="yedD"
                     /locus_tag="ACHKIR_001216"
     CDS             complement(21121..21534)
                     /gene="yedD"
                     /locus_tag="ACHKIR_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YedD"
                     /protein_id="HFO9988978.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     SALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            21721..22926
                     /gene="yedE"
                     /locus_tag="ACHKIR_001217"
     CDS             21721..22926
                     /gene="yedE"
                     /locus_tag="ACHKIR_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HFO9988979.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRA
                     APQIAKEIA"
     gene            22923..23156
                     /gene="yedF"
                     /locus_tag="ACHKIR_001218"
     CDS             22923..23156
                     /gene="yedF"
                     /locus_tag="ACHKIR_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HFO9988980.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            23265..23933
                     /gene="yedK"
                     /locus_tag="ACHKIR_001219"
     CDS             23265..23933
                     /gene="yedK"
                     /locus_tag="ACHKIR_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HFO9988981.1"
                     /translation="MCGRFAQSQTREDYLAFLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPYFIYRADGQPVFMAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAVREWMRQEVGGKEASEIAASGCVPANQFTWHPVSCAVGNVKNQGAAL
                     IQPV"
     gene            24044..24523
                     /gene="yedL"
                     /locus_tag="ACHKIR_001220"
     CDS             24044..24523
                     /gene="yedL"
                     /locus_tag="ACHKIR_001220"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO9988982.1"
                     /translation="MFTIKTDDLTHPAVQALVAYHISGMLQQSPPESSHALDVQKLRN
                     PKVTFWSVWEGEQLAGIGALKLLDDKHGELKSMRTAPNYLRRGVASLILRHILQVAHD
                     RCLHRLSLETGTQAGFTACHQLYLKHGFVDCEPFADYQLDPHSRFLSLTLCENNELP"
     gene            24763..24903
                     /locus_tag="ACHKIR_001221"
                     /pseudo
     CDS             24763..24903
                     /locus_tag="ACHKIR_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003844207.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(24928..25242)
                     /gene="fliE"
                     /locus_tag="ACHKIR_001222"
     CDS             complement(24928..25242)
                     /gene="fliE"
                     /locus_tag="ACHKIR_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HFO9988983.1"
                     /translation="MSAIQGIEGVISQLQATAMSARTQESLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            25457..27115
                     /gene="fliF"
                     /locus_tag="ACHKIR_001223"
     CDS             25457..27115
                     /gene="fliF"
                     /locus_tag="ACHKIR_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HFO9988984.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYRFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESQAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSEESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            27108..28103
                     /gene="fliG"
                     /locus_tag="ACHKIR_001224"
     CDS             27108..28103
                     /gene="fliG"
                     /locus_tag="ACHKIR_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HFO9988985.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            28096..28782
                     /gene="fliH"
                     /locus_tag="ACHKIR_001225"
     CDS             28096..28782
                     /gene="fliH"
                     /locus_tag="ACHKIR_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HFO9988986.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPMVESEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGVAEGRQQGHKQGYQEGLAQGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            28782..30155
                     /gene="fliI"
                     /locus_tag="ACHKIR_001226"
     CDS             28782..30155
                     /gene="fliI"
                     /locus_tag="ACHKIR_001226"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HFO9988987.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGSALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            30174..30617
                     /gene="fliJ"
                     /locus_tag="ACHKIR_001227"
     CDS             30174..30617
                     /gene="fliJ"
                     /locus_tag="ACHKIR_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HFO9988988.1"
                     /translation="MTEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            30614..31741
                     /gene="fliK"
                     /locus_tag="ACHKIR_001228"
     CDS             30614..31741
                     /gene="fliK"
                     /locus_tag="ACHKIR_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HFO9988989.1"
                     /translation="MIRIAPLITADVDNATLPGGKASDAAQDFLALLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSDIVSDAQQADLLIPVDETPPVINDKQSTSTSLTTAQ
                     TITLAAAADNNTAKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPTEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSSVTAAASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTANHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            31846..32310
                     /gene="fliL"
                     /locus_tag="ACHKIR_001229"
     CDS             31846..32310
                     /gene="fliL"
                     /locus_tag="ACHKIR_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HFO9988990.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAAED
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            32315..33319
                     /gene="fliM"
                     /locus_tag="ACHKIR_001230"
     CDS             32315..33319
                     /gene="fliM"
                     /locus_tag="ACHKIR_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HFO9988991.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            33316..33729
                     /gene="fliN"
                     /locus_tag="ACHKIR_001231"
     CDS             33316..33729
                     /gene="fliN"
                     /locus_tag="ACHKIR_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HFO9988992.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSGKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            33732..34097
                     /gene="fliO"
                     /locus_tag="ACHKIR_001232"
     CDS             33732..34097
                     /gene="fliO"
                     /locus_tag="ACHKIR_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HFO9988993.1"
                     /translation="MNNHATVQSSAPVSTAPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRSGRS"
     gene            34097..34834
                     /gene="fliP"
                     /locus_tag="ACHKIR_001233"
     CDS             34097..34834
                     /gene="fliP"
                     /locus_tag="ACHKIR_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HFO9988994.1"
                     /translation="MRRLLSVAPVLLWLVTPLAFAQLTGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            34844..35113
                     /gene="fliQ"
                     /locus_tag="ACHKIR_001234"
     CDS             34844..35113
                     /gene="fliQ"
                     /locus_tag="ACHKIR_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HFO9988995.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            35122..35907
                     /gene="fliR"
                     /locus_tag="ACHKIR_001235"
     CDS             35122..35907
                     /gene="fliR"
                     /locus_tag="ACHKIR_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HFO9988996.1"
                     /translation="MMQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEMFNLLADIISELPLI"
     gene            36197..36820
                     /gene="rcsA"
                     /locus_tag="ACHKIR_001236"
     CDS             36197..36820
                     /gene="rcsA"
                     /locus_tag="ACHKIR_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HFO9988997.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(36864..37052)
                     /gene="dsrB"
                     /locus_tag="ACHKIR_001237"
     CDS             complement(36864..37052)
                     /gene="dsrB"
                     /locus_tag="ACHKIR_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HFO9988998.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            37215..37442
                     /gene="yodD"
                     /locus_tag="ACHKIR_001238"
     CDS             37215..37442
                     /gene="yodD"
                     /locus_tag="ACHKIR_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HFO9988999.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            37741..38556
                     /gene="yedP"
                     /locus_tag="ACHKIR_001239"
     CDS             37741..38556
                     /gene="yedP"
                     /locus_tag="ACHKIR_001239"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050531 - mannosyl-3-phosphoglycerate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0051479 - mannosylglycerate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HFO9989000.1"
                     /translation="MLSIQQPLLVFSDLDGTLLDSHSYDWQPAAPWLSRLREANVPVI
                     LCSSKTSAEMLYLQKMLGLQGLPLIAENGAVIQLAKQWQEIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQLSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPARVWRTQREGPEGWREGLDHFFSAR"
     gene            complement(38553..40247)
                     /gene="dgcQ"
                     /locus_tag="ACHKIR_001240"
     CDS             complement(38553..40247)
                     /gene="dgcQ"
                     /locus_tag="ACHKIR_001240"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator diguanylate
                     cyclase DgcQ"
                     /protein_id="HFO9989001.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRNAV
                     TEFEQHRDEHAWQIELNRRRTLPVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNSSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSENYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRFLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMNSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLLSWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     VGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGANLTQAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVFASDNA"
     gene            complement(40418..40600)
                     /gene="yodC"
                     /locus_tag="ACHKIR_001241"
     CDS             complement(40418..40600)
                     /gene="yodC"
                     /locus_tag="ACHKIR_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HFO9989002.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETDLVPGEGSRSAEEV"
     gene            complement(40679..41596)
                     /gene="yedI"
                     /locus_tag="ACHKIR_001242"
     CDS             complement(40679..41596)
                     /gene="yedI"
                     /locus_tag="ACHKIR_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HFO9989003.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            41769..42689
                     /gene="yedA"
                     /locus_tag="ACHKIR_001243"
     CDS             41769..42689
                     /gene="yedA"
                     /locus_tag="ACHKIR_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HFO9989004.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVESWPPLMMAGVRF
                     LAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     VATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(42678..43148)
                     /gene="vsr"
                     /locus_tag="ACHKIR_001244"
     CDS             complement(42678..43148)
                     /gene="vsr"
                     /locus_tag="ACHKIR_001244"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HFO9989005.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(43129..44547)
                     /gene="dcm"
                     /locus_tag="ACHKIR_001245"
     CDS             complement(43129..44547)
                     /gene="dcm"
                     /locus_tag="ACHKIR_001245"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HFO9989006.1"
                     /translation="MQENISVTDSYSTGNVVQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEVKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(44614..45309)
                     /gene="yedJ"
                     /locus_tag="ACHKIR_001246"
     CDS             complement(44614..45309)
                     /gene="yedJ"
                     /locus_tag="ACHKIR_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HFO9989007.1"
                     /translation="MDLQHWQAQFENWLKNHHQHQDAAHDVCHFRRVWATAQKLAADD
                     DVDMLVILTACYFHDIVSLAKNHPQRQHSSILAAEEARRLLREEFVQFPAEKIEAVCH
                     AIAAHSFSAQIAPLTTEAKIVQDADRLEALGAIGLARVFAVSGALGVALFDGEDPFAQ
                     HRPLDDKRYALDHFQTKLLKLPQTMQTARGKQLAQHNAQFLVEFMAKLSAELAGENEG
                     VDHKVIDAFSPAG"
     gene            complement(45349..45630)
                     /gene="drpB"
                     /locus_tag="ACHKIR_001247"
     CDS             complement(45349..45630)
                     /gene="drpB"
                     /locus_tag="ACHKIR_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DrpB"
                     /protein_id="HFO9989008.1"
                     /translation="MEERLSRSPGGKLALWAFYTWCGYFVWAMARYIWVMSRIPDAPV
                     SGFESDLGSTAGKWLGALVGFLFMALVGALLGSIAWYTRPRPARSRRYE"
     gene            46281..47354
                     /locus_tag="ACHKIR_001248"
     CDS             46281..47354
                     /locus_tag="ACHKIR_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFO9989009.1"
                     /translation="MKRKVLAMLVPALLVAGAANAAEIYNKDGNKVDFYGKMVGERIW
                     SNTDDNNSENEDTSYARFGVKGETQITSELTGFGQFEYNLDASKPEGSNQEKTRLTFA
                     GLKYNELGSFDYGRNYGVAYDAAAYTDMLVEWGGDSWASADNFMNGRTNGVATYRNSD
                     FFGLVDGLNFAVQYQGKNSNRDVTKQNGDGYALSVDYNIEGFGFVGAYSKSDRTNEQA
                     GDGYGDNAEVWSLAAKYDANNIYAAMMYGETRNMTVLANDHFANKTQNFEAVVQYQFD
                     FGLRPSLGYVYSKGKDLYARDGHKGVDADRVNYIEVGTWYYFNKNMNVYTAYKFNLLD
                     KDDAAITDAATDDQFAVGIVYQF"
     gene            47946..48797
                     /gene="hchA"
                     /locus_tag="ACHKIR_001249"
     CDS             47946..48797
                     /gene="hchA"
                     /locus_tag="ACHKIR_001249"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036524 - protein deglycase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase III HchA"
                     /protein_id="HFO9989010.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHGDNPLNGYSICAFPDAADKQTP
                     EIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKVLTGDSPFAANALGKLAAQE
                     MLAAYAG"
     gene            complement(48904..50262)
                     /gene="hprS"
                     /locus_tag="ACHKIR_001250"
     CDS             complement(48904..50262)
                     /gene="hprS"
                     /locus_tag="ACHKIR_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HFO9989011.1"
                     /translation="MKKLSITVRLNLLFILVLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGHGINKIVNRTNVSDGMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIVLCSVLSPLLIRTGLREIKKLSGVTEALNYNDNREPVEVSALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICL
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIQITSFLDTNGSLNIDIASPGTK
                     IHEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLNKHNVFRITLPQ
                     RN"
     gene            complement(50262..50933)
                     /gene="hprR"
                     /locus_tag="ACHKIR_001251"
     CDS             complement(50262..50933)
                     /gene="hprR"
                     /locus_tag="ACHKIR_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HFO9989012.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLRMDSVSQSVSRNNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            51066..51479
                     /gene="hiuH"
                     /locus_tag="ACHKIR_001252"
     CDS             51066..51479
                     /gene="hiuH"
                     /locus_tag="ACHKIR_001252"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HFO9989013.1"
                     /translation="MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            51588..52592
                     /gene="msrP"
                     /locus_tag="ACHKIR_001253"
     CDS             51588..52592
                     /gene="msrP"
                     /locus_tag="ACHKIR_001253"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HFO9989014.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKPAAWQNNLPLTPADKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTHERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYAEQVASLYRGLDLRENF
                     "
     gene            52593..53228
                     /gene="msrQ"
                     /locus_tag="ACHKIR_001254"
     CDS             52593..53228
                     /gene="msrQ"
                     /locus_tag="ACHKIR_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HFO9989015.1"
                     /translation="MRLTAKQVTWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLAALLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLLSLFNRLRKQVHNKLSL"
     gene            53485..54135
                     /gene="zinT"
                     /locus_tag="ACHKIR_001255"
     CDS             53485..54135
                     /gene="zinT"
                     /locus_tag="ACHKIR_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HFO9989016.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYHKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            complement(54805..54894)
                     /locus_tag="ACHKIR_001256"
     tRNA            complement(54805..54894)
                     /locus_tag="ACHKIR_001256"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(54858..54860),aa:Ser,seq:cga)
     gene            54988..55785
                     /gene="mtfA"
                     /locus_tag="ACHKIR_001257"
     CDS             54988..55785
                     /gene="mtfA"
                     /locus_tag="ACHKIR_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HFO9989017.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            55886..55961
                     /locus_tag="ACHKIR_001258"
     tRNA            55886..55961
                     /locus_tag="ACHKIR_001258"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:55919..55921,aa:Asn,seq:gtt)
     gene            56274..57047
                     /locus_tag="ACHKIR_001259"
                     /pseudo
     CDS             56274..57047
                     /locus_tag="ACHKIR_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter beta domain-containing
                     protein"
     gene            57097..58032
                     /locus_tag="ACHKIR_001260"
     CDS             57097..58032
                     /locus_tag="ACHKIR_001260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="invasin domain 3-containing protein"
                     /protein_id="HFO9989018.1"
                     /translation="MAGPADASQSVLKNNRSSLKGDFTESAELYLVLHDLSGHPINVS
                     EGLEFVQSGTNVSYVQISTIDYTQNLYGEYKATVTGGGEGIATLIPVLNGVHQAGLST
                     TIEFISAGTRPMNGTVSVNGANLPAASFPSQGFTGAYYQLNNDNFAPGKTAADYAFSS
                     TASWVDVDTSGKVTFKNVGDRNAVIITATPRSGGAIYQTQVRVKGWWVNHGNNLMQLS
                     QAENYCSNQVGNGYPLPRADLLSNGHMRREIGSLYGEWGDMGNYMKEADFYSMVYWSS
                     NSAGAGQQYIVSLETGTQNTYQTYEFFYGACYKQI"
     gene            complement(58294..59346)
                     /locus_tag="ACHKIR_001261"
     CDS             complement(58294..59346)
                     /locus_tag="ACHKIR_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HFO9989019.1"
                     /translation="MFLASLLRRIAFSYYDYKAYNFNIEKTDFVVIHIPDQIGDAMAI
                     FPVIRALELHKIKHLLIVTSTINLEVFNALKLEQTKLTLVTMTMQDHATLKEIKDLAK
                     NITQQYGTPDLCIEAMRKKNLKTMIFISQLKAKTNFQVVGLTMKCYSPLCKSASRMDQ
                     NLRAPVPMTWAFMMREAGFPAVRPKYELPLSKDVLDEVREEMRSLGSYIALNLEGSSQ
                     ERTFSLSIAENLIEKIQSETDIPIVIVHGPKGEEKARVLVDCYNNVFRLSLSPSIKRS
                     AAIIKDAYIAITPDTSILHMASAYNTPVVAIYADYKTRWPAMADVSESVVVGQKIDNI
                     SLDEFAKALKSVLLRI"
     gene            59661..60977
                     /gene="shiA"
                     /locus_tag="ACHKIR_001262"
     CDS             59661..60977
                     /gene="shiA"
                     /locus_tag="ACHKIR_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HFO9989020.1"
                     /translation="MDSTLISTRPDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRAIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQHNQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGTLSAFPFF
                     MALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASYRYSGAGVGYQVASVVG
                     GGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAMTALLMKDNQRA"
     gene            61079..62533
                     /gene="amn"
                     /locus_tag="ACHKIR_001263"
     CDS             61079..62533
                     /gene="amn"
                     /locus_tag="ACHKIR_001263"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HFO9989021.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGKYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
BASE COUNT        15773 a        15727 c        15056 g        16120 t
ORIGIN      
        1 tattcttatt tcagatcatc gtcagaattg actccacgat cacatttcgg accggcagaa
       61 aggaattatt ctgcaaacag taattatggt gttttgattt atcttgcacc tctccacttc
      121 tggatataag gatattaggt atggcaaccg ctggaatgct tctcaaactc aactctcaaa
      181 tgaaccgcga gttttacgca tccaatctct accttcacct gagtaactgg tgttctgaac
      241 agagtctgaa cggcaccgcc actttccttc gcgcccaggc acagagtaat gtgacccaaa
      301 tgatgcgcat gtttaacttt atgaagagtg tcggcgctac ccccatcgtt aaagccattg
      361 atgttcccgg tgaaaaactg aactctctgg aagaactgtt ccaaaaaacg atggaagaat
      421 acgagcaacg ttctagtacg ttggcacagt tagccgatga agcgaaagaa ctgaatgatg
      481 attcaaccgt caatttcctg cgcgatctgg aaaaagaaca gcagcatgat ggcctgttgc
      541 tgcaaaccat tcttgatgaa gtgcgcagtg cgaaacttgc gggtatgtgc cctgtgcaga
      601 ccgaccaaca tgttctgaat gtcgtgtcgc atcagctaca ttgatcatca tcggcgctaa
      661 tgcattgcgc cgatgaagtt tttgagaaac cgctgcctca tttttttgaa gcagcggttt
      721 tttaatggga ttcgccctgt ggggtaaact tgagttcaat caacgcgatg gctttttgga
      781 ttgcccgcat ggtgaccggg tctgcggcgg cgggatggtt agtaaagtcg atattcttta
      841 gctggctgga cattttttcg cgaacttcaa cgggtgcgat cacatcgaga acatccagaa
      901 tttgtttgat aaccaactgg caagcaacca catcagaaac cagttcctga tcggcattca
      961 gcggctggga catcgtaaac tcctgatagc attttgaaag ccgttatagt agcggcttca
     1021 catcttcagc gatagtcaca tccaccgtca tcaggacaca aaaaaacctg ccggagcagg
     1081 ttttttgtta tcggaacata ttgcctggcg gtacgtcttt gaacgtcttg caatagttat
     1141 tgaacatact tttcaggatt ttgcgcagtt tcatcgcggc actccgacca tttgttatac
     1201 aggtgttatt gtctttgcgc ttataatgtg acaaccatca caaaaatcaa tctttatgtg
     1261 atgcaaatca cataaatacc cctttaatgt tataaaaatg ataatcaaaa agcagctacc
     1321 ccatttctga cacctacaga tggcaagaaa tagcgcctgc caggcgtctt ttccggccat
     1381 tgtcgcagcg ctgtagcgcg taaaatagtg ctttctttta ctcttctggc tggaccatga
     1441 gacttctgat tctgactctt tcattaataa cgctggcggg gtgtacggtt actcgccagg
     1501 cccatgtgag cgaagttgac gcggcaacag gtattgttcg gttggtttat gatcaggctt
     1561 ttttgcagca tgcccgtacc gatcgttatg tcagtcgcgg gatcgccgat cgcgcatgcc
     1621 agcaggcagg ctatacccac gctatcccct ttggtcagcc agtaggcaac tgtagcctct
     1681 ttgcgggttc cttatgtttg aatacggaat tcactttgtc atatcagtgc catcattccg
     1741 ccttccctgt ctttctctaa acgttttaat cagcgagggg gatcttcgct gattaaataa
     1801 atagcggtaa tgcgttttat tcccattcgt atttttaata aatgatgttt atattttacc
     1861 ttttgcaaat aataaaataa caaattatag tgacgccaca gcaacaaata taacctttgt
     1921 ggagcactat catgctgaaa ccagaaatga ttgaaaaact taatgagcag atgaacctgg
     1981 aactgtactc ttcactgctt tatcagcaaa tgagcgcctg gtgcagctat cataccttcg
     2041 aaggtgctgc cgcgttcctg cgccgtcacg cccaggaaga gatgacgcat atgcagcgtc
     2101 tgtttgatta cctgactgat accggcaact taccgcgtat taataccgtt gaatctccgt
     2161 ttgctgaata ttcctcactt gatgaattat tccaggaaac ctataaacac gaacaattaa
     2221 tcacgcagaa aattaacgaa ctggctcatg ctgcaatgac caatcaggac tacccaacat
     2281 ttaatttcct gcaatggtat gtttctgagc agcatgaaga agagaaactg ttcaaatcga
     2341 ttattgataa attaagcctg gcaggcaaaa gtggcgaagg tctgtatttt atcgacaaag
     2401 aactctctac cctcgacaca caaaactaat gctctccggc ggcaggtttt ctgccgccgt
     2461 ttcaaatcag tgacggcaaa tcttactctg atcggttgaa aaaccttctt ctgaaggtat
     2521 aaatttgcct tttgctgcca gaaacgccac caactctccc gcagtcatcc cttctgccga
     2581 gcaggtgtga aaacgtgcct gttcaccaaa acgcgcttta attgctgctt ccagactggc
     2641 atgcgtatat tgctcgcctg attcaatcat catatttaac acttcgtgac cgtgaataga
     2701 gtccatcgtt cctcctcaaa aaaagcctag cgtagcgatt gccgcttatg aagactttgc
     2761 gccagcgcag aactgaacgc ttttattgta catttatatt tacatcatat gtaacgccgg
     2821 tttgacgaat cagccgttat gccttaacct gcgccgcaga tatcactcat aaagatcgtc
     2881 aggacagaag aaagcgtgaa aaacagaact ctgggaagtg tttttatcgt ggcgggaacc
     2941 acaattggcg caggcatgct ggcaatgccg ctggctgcgg caggtgttgg ttttagcgtc
     3001 acgttaatct tgttaattgg gctttgggcg ttgatgtgct acacggcgct attactgctg
     3061 gaggtgtacc agcatgtccc ggcagatacc ggtctgggca cgctggcaaa acgctatctg
     3121 ggacgctacg gtcagtggct gacgggcttc agtatgatgt tcttaatgta tgctctgact
     3181 gcggcataca tcagcggtgc cggtgaattg ttggcctcca gcatcagcga ctggacaggt
     3241 atttcaatgt cggcaaccgc tggcgtgctg ttgttcactt ttgttgccgg tggcgtggtt
     3301 tgtgtcggaa cttcgctggt cgatttattt aaccgttttc tgttcagcgc caaaattatt
     3361 tttctggtgg tgatgctggt attgctgcta ccgcatattc acaaagtgaa tcttttaacc
     3421 ctgccgttgc aacagggact ggcactgtct gcaatcccgg tgatttttac gtcgtttggt
     3481 tttcacggta gcgtgccgag tattgtcagc tatatggatg gcaacattcg taagctacgc
     3541 tgggtgttta taatcggtag tgcgatcccc ctggtggcat atattttctg gcagctggcg
     3601 acccttggca gcattgattc aacaaccttt atgggattgc tggctaatca tgccggatta
     3661 aacgggctgt tacaggcgtt acgcgaaatg gtggcctctc cgcatgttga gctggcagtg
     3721 catttatttg ctgatttagc cctcgccacg tccttcctcg gcgtagcgtt aggcttattt
     3781 gattatctgg cagatttgtt tcagcgttca aataccgttg gtggacgatt gcaaactggt
     3841 gcaattacct ttctgccgcc actggcgttt gcactgtttt atccacgagg atttgtgatg
     3901 gcgctgggtt acgccggtgt ggcgctggcg gtactggcat tgattatccc ttcgctgttg
     3961 acctggcaaa gcaggaagca caatcctcag gcgggttacc gggtgaaagg tggtcgtccg
     4021 gcgctggtgg tggtgtttct ctgtggtatt gctgtgattg gcgtgcaatt tttgattgcg
     4081 gcaggcttgt taccagaagt ggggtgatta gattcttatt gggtgccaga attaacgctg
     4141 acacccaatt tgacctctta atgcaggcag cactgcttaa atttcttacc actaccgcac
     4201 ggacaaggat cgttacgccc cggtttctct tctgctttga taggttgctg aacagctttt
     4261 tcctgcggat gcgccatcca gtatgcatgt aaatcaagcg ccgccagtcg aatagcatct
     4321 acgctctctt caaacgcttc tggcgacatc ttctctaccc gctcgaagtt ttcctcagta
     4381 ccgtgcagcg caatcgcatc cagcgctggt tttaacgaat cgggcaacgt tgaccagtca
     4441 gaaagtgcca cgccccgcat atagccaaag caccactcct caacaatcgt cagctcgctg
     4501 ccatcgactt ctcgcaagcc gaataacggc tcaaactgct ccgggaattc gttcagacgc
     4561 tctgcggtat cagccatatg ttgaaaagcc aggttcataa agcgcgtcat ctctttctct
     4621 gacgcccagc gcggcacata gtcagcccca ccccacacgg caaccagcca ctgtgccgat
     4681 tcaatctctt gcggagaact caacaccgcc gtcaataaac cgtccagctc cgccacatca
     4741 agaatggcgt ggtcagtgtt gtatttggtc agaatatcgt ccagccattc caactcactt
     4801 tcgtttaacg gtcccgtttt catacgcttt tccttgtgga tctcaactcg ccagcaccta
     4861 tcttacatgc cggtccgtat cagagatact ttttgagtgg ctttgccggt gattaaaaat
     4921 taaggagggt gtaacgacaa gttgcaggca caaaaaaacc acccgaaggt ggtttcacga
     4981 cactgcttat tgctttgatt ttattcttat ctttcccatg gtacccggag cgggacttga
     5041 acccgcacag cgcgaacgcc gagggatttt aaatcccttg tgtctaccga ttccaccatc
     5101 cgggctcggg aagaaagtgg aggcgcgttc cggagtcgaa ccggactaga cggatttgca
     5161 atccgctaca taaccgcttt gttaacgcgc caaattcttc aggcctttca gccagacatc
     5221 cgcttgacgc cgatgtcttt taaactggag cgggaaacga gactcgaact cgcgaccccg
     5281 accttggcaa ggtcgtgctc taccaactga gctattcccg cattcatcaa gcaatcagtt
     5341 aatcacttga ttttattatc gtctggcaat cagtgccgcc gttcgatgcg ttgcattcta
     5401 cttacctggc gcgatgagtc aacgatattt ttcaccactt ttgatcgttt gctgaaaatt
     5461 gcgccgaaac gatcactgat caagcaaatc tgcacgcgca gcgctcaaat attgcaacat
     5521 tgaccacaga gtcagtaccg cagccacaaa gaaaagtgca ataccggcgt actcaaccca
     5581 aatgttcgga cgccacagca gccaggccag cgccaccatc tgggcagtgg ttttcacttt
     5641 cccaatccag gagacagcca cgctactgcg tttacccaac tccgccatcc attcgcgtaa
     5701 cgcagaaata ataatttcac gggcgatcat cgttgctgcc ggtaaggtca cccaccagct
     5761 gtgataatgc tcggttacca gcaccatggc gatagccacg agaactttat ctgctacagg
     5821 gtcaaggaaa gcaccaaacc gggtactctg gttccagcgg cgtgccagaa aaccatcgaa
     5881 ccagtcagtc accgccgcga cgcagaaaat gagcgcggcg gcaaacggcg accaggtgac
     5941 aggcagataa aagaccaata caaagaatgg gataaggatg acacggaaca gtgtaagcaa
     6001 cgtagggata ttaaattgca taatgacggg taactatctg ttgtcagtaa gattacccct
     6061 atgttgctac agagacatca atgtttcaac gaccagaaga tcttttctgc cagaccttgc
     6121 gaaatacccg gcacttttgc aatttcctcg acgctggcgt tacgtaaacc ttgcaaaccg
     6181 cccatatatt tcaacaacat ttgccgacgt tttggcccga cgccttcaat ggtttccagg
     6241 gaactggtat ttttgacctt cgcccgtttt ttacggtgcc cgccaatcgc gtgatcgtgt
     6301 gattcatcgc gaatatgctg gataacatgc agtgcgggag aatctggcgg caaactgaat
     6361 ccctcacctt ccggctcaaa gaacagcgtt tccagcccag ccttacgatc tgctcctttg
     6421 gcaacgccta gtagcagcgg atgattttta tcccatgaga catccagttc ggcgaaaaca
     6481 tttttcgcct gcgcaagttg gcctttgccg ccgtcgataa ggatcacatc cgggatctta
     6541 ctctcgtcga tggctttacc ataacgccga cgcagcacct gattcatcgc cgcataatca
     6601 tcgcccggcg tgatgccagt aatgttatag cgtcgatact ccgcacgcag cgggccgtta
     6661 gcatcaaaca ccacacagga agcgacggtt tgttccccca tggtatggct gatgtcaaag
     6721 cattccatcc gcttcacttc cggcaatttc aacacgctgg caagcgcggt cagtcgctgg
     6781 tgaacggtag attgctgcga aagtttgctg gttaaggccg tcgccgcatt ggtgcgcgcg
     6841 agtttcagat aacgcgcccg atcgccgcga ggtttggttt gaacattaat cttgcgtccc
     6901 gccagttctg aaagggaatc ggcgagcagc gttttatcgc taagattaaa atcgagcagg
     6961 atctcacccg gtaaggtgcg catctggctg ccttgtaaat agaactgacc tacgaaggtt
     7021 tccaccacct cgctcagttc cgtaccgcca ggcactttcg ggaaatagct gcggctgccg
     7081 agcactttac cctgacgaat gaacaatacg tggacacaag ccatgcccgc atcgaacgcc
     7141 acaccaataa cgtcgaggtc gtcgccggta ttggacacaa actgtttttc ggtgacgcgt
     7201 cgcaccgcct gaatttggtc gcgaatacgt gccgcttctt caaactccag attctggctg
     7261 gcagtttcca tacgactaat gagttgcgta agcacctgat catctttgcc agacaaaaac
     7321 aggcgcacat actcgacctg ctgagcgtat tcttcttcac tcaccagtcc ttcaacgcac
     7381 ggtcccagac agcgccctat ctgatattgc agacacggac gcgagcgatt gcgataaaca
     7441 ctattttcgc actggcgaat ggggaatatc ttttgcagta gcgccagtgt ttcacgtacg
     7501 gcatagccat tcgggaacgg gccgaaatat tcacctttag catgcttcgc accacgatgc
     7561 atcgccagac gcggatgggt atcgccactc aggaagataa agggataaga tttatcatcg
     7621 cgtagcaaaa cgttgtaacg cggctgatag agtttgatgt agttgtgttc cagcaacagc
     7681 gcttcggttt ctgtatgagt aaccgttaca tcaatttgct ggatctgggc gaccagcgct
     7741 tcggttttgc gcgaagcgag gttgctacgg aaatagctgg aaagccgttt tttcaggtct
     7801 ttcgctttgc cgacatagat aaccgtacca ccagcatcgt acatgcgata aacgcctggc
     7861 tggctggtta cggtttttaa aaacgctttt gcgtcaaact gatcactcac tgacttgata
     7921 atgtctccgc attacacaga ccatggcgaa ttgccaggtg agtcagctca acatcgccat
     7981 gaatgtttag tttactgaac atacgatagc ggtagctgtt caccgttttc ggactgagat
     8041 tgagctgttc tgagatctca ttgaccttct ggcccttggt gatcatcagc ataatctgca
     8101 attcacgttc agacaaactg gcaaatgggc tttctgtttt ttctggttcg atctggctta
     8161 acgccatttg ttgagcgatg tcagaagcaa tgtaacgctg ccctgaatag acagaacgga
     8221 tcgcactcac aacttcctgc ggagccgcgc ctttgctgag gtagcccgca gcaccggcct
     8281 gcatgacttt cgctggtaaa gggttttctg tatggacggt aagcatgatg attttaacat
     8341 cagctgtgga acgcgcgatt ttacgcgtcg cctcaaggcc gccaatgccc ggcatactca
     8401 tgtccattag caccacgtca acggcatttg cccggcacca tttaacggcg tcttcaccgc
     8461 acgatgcctc tccgacgact tttataccct ttatatcttc cagaatgcgt cgtatccctg
     8521 cgcgcaccag ttcgtggtca tcaacaagta gaacgttgat caaaggaata tctccagaaa
     8581 tagggataac gctactgata gttagtcatt cgtatattag cggtttttat tgcaactttg
     8641 aaacgttaaa aatggtgggt attcgatttt tctctcgttt ttgtaaattc gattgtccac
     8701 agttatggtc acgcccgtca ccaacggctt ccgggatcgt ttcactgtaa gccctgcgat
     8761 gaaatgtgac aaaaattgac tttattcagc aaaaatgaaa atcagcccgc tgaatatgta
     8821 acattaatta acccgaacaa tacgggcaaa aaacattcat gataaaaata tttattggtc
     8881 attatattaa cgtattttat agcactgccg atatcacgct caaaaaacaa ccactgctat
     8941 ttttagcaaa gcttatggta tactccgccg ccttaacatt tttcaccgca aattttcatt
     9001 gcaacatgac gaggaaaata aatgagtacg cctgattttt ctactgccga gaataatcaa
     9061 gaactggcaa atgaagtctc ctgcctgaaa gcgatgctga cgctgatgct gcaggcgatg
     9121 ggacaagctg acgcgggccg cgtgatgtta aagatggaaa aacagcttgc gctgatcgaa
     9181 gacgaaaccc aggctgcagt attttccaaa acggttaagc aaattaaaca agcctaccgt
     9241 cagtaatata aaaccggctg agagcgtgcc tttcagccgg tttttgcatc tggcacgcag
     9301 aaagaaaagc gatcaaatta agccagtagc ggccgcgtaa caggccacct gggtcttatt
     9361 tggtgcattg atttttttct gcatgttttt ctgatggaaa ttgaccgtat tctcagagat
     9421 tgacaaaatc atcgctatct ctgctgatgt cttcccttcc gccgtccatt tcagaatttc
     9481 tttttcgcgc ttgctgaaat tcatctctgg cgtcatcact atttcatcat ttaaacgcat
     9541 cagagccatc agactttcgc gcaccagtaa ctgcattttt aattgcagtt catcactaag
     9601 aatgggtatt tcgcgcgcag tgcaacggga aaaggacaaa aagcccagcg cccggtttgg
     9661 cagcattaaa tactgagtga caccgcggcg taaaccatgc gcgcgcgcag cttcccataa
     9721 cggctgtgct tcgctgaata agtcatcatt ccacattaaa tggccctgac taaagttttc
     9781 agggttcagt acaggatcaa ttgcgagaaa gttttttgcc tgataataac taacccacgc
     9841 ctcagggtaa ttggtgtaga aagccacttt aggtcgcgtg aatggtaccg ggtggcgaac
     9901 acataacgaa tagtaatcgt actccagctg ctgagcctga agctctattt catggtagac
     9961 ctcttctgcg gcctccatcc tctgaaaacg caacagcatc gtgcgacgcc agctgaaaaa
    10021 atccgtatcc tgcatagtaa accgcaacgc ccctgagagt gagtatcatt tataatgata
    10081 attcaaacta tcacataaat aagatttata tataatttat attattcagg caatgaatta
    10141 cttttgcaag ccatcgcatt ctcttatgtt attaatgagt tatgctgatt tgttaagcag
    10201 ttttatcacg tttgaagtgg cgcaccgccc cgacaggtga atcgtcgggg ctgatttttt
    10261 cttattattg cagcagaaac ttctcgagga actggcgggt gcgaggctgc tcgggatcgg
    10321 caaataacgc ttttgcggcc ccctgctcga ctatccgccc ctggtccata aagatcgccc
    10381 ggtccgcaac atcccgggca aagctcattt cgtgcgtcac aatcaccatc gtgcgctttt
    10441 cctgcgccag ctggcggatg gtgttcagga cttcgcccac cagctctgga tccagcgccg
    10501 acgttggctc gtcaaacaaa atcacttcag gacgcatagc cagcgctcgc gcaatcgcga
    10561 cacgctgctg ttgaccgcca gacaaacgac gtggatagct ggtttcttta cctgccagcc
    10621 caactttcgc cagcagctcg cgagcgcgcg ccgtggcctc ttctttcggt tcgcctttga
    10681 cgatcaccgg cccttcaata atgttttcca gcaccgtacg atgcggaaac aaattaaagt
    10741 tctggaagac aaacccgacg tgctgacgca actggcgaat cagcgatttt tgctgactta
    10801 atgaacgtgc agtatcaatg gtgatatcgc caacggtgat ggttcccgct tccggttgtt
    10861 ccagcagatt tatgctgcgt agcaacgtgg ttttgccgga accacttgga ccgataattg
    10921 ccaccacttc gccaggcttc acctcaaggt cgataccgtg cagcaccgtc tgaccgtgga
    10981 attttttcac caggttctta acttcaatgg cactcatttt ggttctctct cctggcgatt
    11041 aagttgattc tcaaaatggt tctgcaacgt cgataatact gtcgccatga tccagtagat
    11101 gagcgaagcc gccaggtaca tggtgaacac ctccagcgtg cgcgaggtaa tcaactgcgc
    11161 ctgacggaac agctccggca cctggattgt cgcggccagc gacgtatctt ttaccaggct
    11221 gataaagctg ttcgacagcg gcggcaacgc cacacgcgcc gcctgtggca aaatcgcgcg
    11281 acgcatggtc tgccacggcg tcataccaat actggcagca gcttcccact gacctttatc
    11341 aatagaagaa atggcggcgc gcagtgtttc ggcggcatag gcggcagtgt ttagcgacag
    11401 accaatcatt gctgacggaa tgggatctaa ttcgatgcca aactgcggca ggccgtaata
    11461 gatcataaac agttgtgcga tgagtggcgt accacgaaaa atagagatat aaaaacgcgc
    11521 cagccagcgc acgggccaga ttggcgaaag gcgcatcagc gcgagaataa acccgagcag
    11581 taagccaaaa aacatgccgc cgatactgag ttgcagcgta tacccagccc ctttcagcag
    11641 gaacggcaag gaatcaataa ccagttgtat actttcttgc atgagcgttt ccggagctaa
    11701 acgtggggat gataggcgaa cagcgcaggc gcgccgccgg tatgaataaa cagaatcggc
    11761 ccttcatctt tgaagcgttt ctgactgata ccgtcaatca gccccgccat tgcttttccg
    11821 gtatacacag gatcaagcag aatgccttcc agccgcgcca gcaatttcac tgcttccatg
    11881 ccttcgtcgt tcggcacgcc gtagccaggt gcaaaatagt catcccagag taaaatttcc
    11941 gctgatgcgg tcagctccag ttctttcgca atcgcctgtt ggaggttaac cactttcggc
    12001 aattgatcgg caacggaacg cgacacggtc acgccaatca gttcgctttc aggcatcagg
    12061 tgttccagcc caacagccag tccggcgtga gttccggcac tgcccgacgc gaccaccacc
    12121 gacgaaatat taactgcccc ttcacactgt tgcgcaattt ccagcgcact ctccacataa
    12181 cccagcgcgc ccaaagcatt agaaccgcca accggaatga catacgggcg aaagccttgt
    12241 gcttcgactc gcgtcgccag ctcttccagt tgggcattgg gatcggtcag tgcgtcgcac
    12301 atttcaatct gggtattgaa cagatccagc aacaaacgat tgccgttggt taaatagttt
    12361 tctgcggttg tgccaatagg attttccagc agcgccacgc agtgcagacc gattttcgcc
    12421 gcaactgcgg cagtctggcg cacatggtta gactggatcg ccccggcagt aatcagcgta
    12481 tcggcacctt cacgcagagc atctgccgcg agaaattcca gcttacgtaa tttattgccg
    12541 cccattgcca tgggagtgac gtcatcccgt ttgatgaaaa tttcccgtcc aagataatca
    12601 gaaaagcgcg gcagatattc gagcggcgtt ggcgcgccga taaactccag ccgtggaaaa
    12661 cgggttaaat tatgcagtgg cataacagcc tccgatgtgt gttgttgtga ttttcttatt
    12721 atgcacgctg aaaacgcgta aataaaaaag gcgctagtga aagcgccctt ttttgtcatt
    12781 atgctgatta tttggtcaca tcagcaccaa accatttttc ggaaagggct tgcagagtgc
    12841 catctttttg catttccgca attgcatcat tcaccgcttt cagcaggtct tcatttcctt
    12901 tacgcagcgc cacaccagac tcctgacggg agaatgcttc accggttact gccagcgtat
    12961 cgttggtttt cttcaccaga tccagcgccg ccagacggtc aacgaggatc gcatcgatac
    13021 ggcctacgcg cagatcctga tatttggtcg ggtcatcatc ataggtacgc acatcgacgc
    13081 cctgaacatt ctgacgcagc cactcttcat agttggtgcc cagaccgaca cccacttttt
    13141 tgcctttcag atcatcagcc gttttaatgg tgccttcgtt accttttttc accagcgcct
    13201 gaataccgga aatggtgtac ggggtagaga aatcgtattt tttcttgcgc tcgtcagaaa
    13261 tggtgacctg gttaatcacc acatcaatgc gtttagagtc cagcgacgcc agcataccgt
    13321 cccatttagt cggttttagt gacgcctcaa cgccaagatg ttttgccagc tgttgggcaa
    13381 attccacttc aaaaccggtt aatttgccgt catccccctg aaaactgaac ggcggataag
    13441 ttccttccag ccctaccagc agcgtgccgc gctctttgac tttattaagc agaccttcat
    13501 ctgcaaaact tttaacgctc atacccgcaa ccagcgccac ggccatcaca cccatcaatg
    13561 cctgacgtcc cagatgtgct aatttcatat ttaccccgaa tgttgttatg tctgtttgca
    13621 gtgtagagcc attgtttgta aacacaaaaa caactccgct acatcttatt cttatttaat
    13681 atatatcaga agcaggcaga ctggaggatt tctgcacaag tccggtacac gtttgacgct
    13741 gatagtgttg atacttccgt aacgcaatgc ggtaattgtt ggtgctggtg gtgggcagcc
    13801 acggggcgag gatttcatca aggaaaccgt cctgcagctg atcgagggaa atattttgct
    13861 cgtgtagatg attacccaaa cggcgcagac gaacgacata ttcacgcacc gtacctgggc
    13921 tcatttcagt ttgctcaaaa agaaattgct taaagccgat aatatcgaaa aaatcactct
    13981 gcgttttgca atgtaaatca ccgcaaaatc ggcacaatgc cgcccatgat ttttgttcca
    14041 cttgccagcc attttcatca agcagcatgt ccaggcggga aatctcgatt ttattcacta
    14101 ttttgccgtc gcgaacgagg gtaatgcgat cgagtaattt acggcaatgc gcgcaatggg
    14161 tctggctgtg tttaaagtct ttaagatagc ggcttaatgg ccgtcttttc aggtgctgca
    14221 ccatcattaa gaactcctgg tagtcaaaat taaagtgcgg catttactga cgttataact
    14281 tacccagttt agtgcgtaac cgtttaatgg cctggctgtg taactgactg acccgcgatt
    14341 ccccgacctc cagcaccgcg ccaatctctt tgagattcag ctcttcctgg taatagaggg
    14401 ttaataccag tttttcgcgc tccggcaacg tttcgatagc ttccatcacc cgctggcgca
    14461 gattactgtc cagcagttgt tgtagcgggt tttctcgctg atgatcatca gtaaccagtt
    14521 cgatgctatc gccgtgctct tcgcgccact catcgtagga gaagagctgg ctgttattgg
    14581 tgtcgagcaa catttggcga tagtcggcaa tatcgatccc taaacgttcc gctacctcag
    14641 tttccgtggc gttgcggcca agttcctgct ccagttgccc tattgcctgt gccacttcac
    14701 gcgcgttgcg tcgcacgctg cgcggcaccc agtcacggct gcgaagttca tccagcatag
    14761 cgccacggat acgctgcact gcgtaagttg taaatgccgt tccttgtagg gcgtcatagc
    14821 gttcgacggc attgagtaac ccaatgccgc ccgcctgtag cagatcgtca agttccacgc
    14881 tcgcgggcag tcgaacctgc aggcgcaatg cttcgtgacg caccagcggg acataacgct
    14941 gccacagcga gtgtttatcc attacacctt cagcggtata gagtgaattc acgataaaca
    15001 gccctgcgtt atatgagtta tcggcatgat tatccgtttc tgcagggttt ttaatcggac
    15061 gattagtggg tgaaatgagg ggttatttgg gggttaccgg taaattgcgg gcagaaaaaa
    15121 ccccgccgtt ggcggggaag cacgttgctg acaaattacc attcatgttg ccggatgcgg
    15181 cgtaaacgcc ttatccggcc tacaaaatcc gcaaattcaa taaattgcaa ttcaccttgt
    15241 aggcctgata agcgcagcgc atcaggcaat ttggcgttgc cgtcagtctc agttaatcag
    15301 gttacgacga ttaaccctgc agcagagaca gaacctgctg cggtacctgg ttagcttttg
    15361 ccagcacgga gttaccggcc tgctggatga tctgcgcttt cgacatgttg gacacttcgg
    15421 tcgcatagtc ggcgtcctga atacgggact gcgcttcgga caggttggta gtggtgttgt
    15481 tcaggttggt gaccgcggaa tccagacggt tttgcaccgc accgagggag gagcggaatt
    15541 tgtcgatgga gctgatggct tcgtccagag ctttcagtgg atcggcggtg gttgcagctt
    15601 tagtttcagc gtcagtagta aaattaccat ccgctgaaac atagactgct ttacctgaac
    15661 cattggtcac agttccgtct ttttgcaaaa acagattggt tgtattatcg gtagtcagtg
    15721 aaccatcagc tttggtgtaa gcgtcttcaa aaactgtacc tgtgtattgt tgtactatac
    15781 ctttactatc aactttaata tcgcctgcgc tggtaccagt agctccaaca tcaactgtga
    15841 atcctgtgga cttagttgcc gattgaagag actcagcact cattcttgca tttgctacag
    15901 tgattgcacc agaagtagta gcatcagtac cgccatccag agtgaaggtt gtttcatctt
    15961 cagttgtaat taccgtagat acggcacccg gtgtgcctgt tgtatgccag tttttagtca
    16021 gtgcatcaag agtggctgct ttgacagtcc cagcattgtt ttgagtgagg ttaccatcta
    16081 cgtcaataaa tagttctgaa ccatctttcg cggtaatttt accgtcgcta gccaatacaa
    16141 cgtcaattga cgtagaacca attttaacgt ttaagttagc agtatcgccc gcttttggtg
    16201 tcaggaagga ttgtaactga gcactgttac tatcaccaga atttgtacca gctgcaatag
    16261 ttgcactata tgtaaaatca cctgtagcgc tgttgtatgt ataatctgta gctgttggcg
    16321 atccaaaacc atttgcaatt gtggcattaa cttttgcgtt attagccaaa ctcgcaatta
    16381 catctgcagc tgtagatgtt ttcaggcctg cgctaacagt ataggtaact gtaccattag
    16441 catcagcagt acctggagcc aagagttgag ctgctgccaa atcagattta gtcgctgcag
    16501 tattagccac agccccgcca ccattcacat taaacccact cagccccagc gtatcagagt
    16561 caattttctt cagatcaata gtgatagtct ggccgtcatt cgcaccaacc tgaattttca
    16621 ttgaaccgtc tttcgccagt acgttcacgc cgttgaactg ggtctggcca gatacgcggt
    16681 caatttcgtc cagacgggat ttgatttcgt cctgaatgga gtccagatcc gaatcggagt
    16741 tagtcccggt agaagcctga accgtcagtt cacggatacg ctgtaagttg ttgttgattt
    16801 cggacagcgc gccttcggtg gtctgtgcaa cggagatacc gtcgttggcg ttacgtgcag
    16861 cttgagtcag acctttaatg ttagaagtaa aacggttagc aatcgcctga cctgcggcgt
    16921 catccttcgc gctgttaata cgcaagccag aagacagacg ctcgatagaa ctcgacagcg
    16981 cagactggtt cttgttgata ttattttgag tgatcagcga gaggctgttg gtattaatga
    17041 cttgtgccat gattcgttat cctatattgc aagtcgttga ttacgtattg ggtttccacc
    17101 cgtcggctca atcgccgtca accctgttat cgtctgtcgt aaaacaacct ttagaatttt
    17161 tttcacaaac agccattttt tgttagtcgc cgaaatactc ttttctctgc cccttattcc
    17221 cgctattaaa aaaaacaatt aaacgtaaac tttgcgcaat tcaggccgat aaccccggta
    17281 ttcgttttac gtgtcgaaag ataaaaggaa atcgcatggc aagtatttca tcgctgggag
    17341 tcgggtcagg tctggattta agttcaatcc ttgatagcct caccgccgcg caaaaagcga
    17401 cgctaacccc tatttcaaat cagcaatcgt cgtttaccgc taaacttagc gcctacggta
    17461 cgctgaaaag cgcgctgacg actttccaga ccgccaatac tgcattgtct aaagccgatc
    17521 ttttttccgc caccagcact accagcagca ccaccgcgtt cagtgccacc actgcgggta
    17581 acgccatcgc cgggaaatac accatcagcg tcacccatct ggcgcaggcg caaaccctga
    17641 ccacgcgcac caccagagac gatacgaaaa cggcgatcgc caccagcgac agtaaactca
    17701 tcattcaaca aggcggcgac aaagatccga ttaccattga tatcagcgcg gctaactcat
    17761 cgttaagcgg gatccgtgat gctatcaaca acgcaaaagc gggcgtaagc gcaagcatca
    17821 ttaacgtggg taacggtgaa tatcgtctgt cagtcacatc aaatgacacc ggccttgata
    17881 atgcgatgac actctcggtc agcggtgatg atgcgctaca aagttttatg ggctatgacg
    17941 ccagtgccag cagcaacggc atggaggtct cggtcgccgc ccagaatgcg cagctgacgg
    18001 tcaacaacgt cgccattgag aacagcagca acaccatcag cgacgcgctg gaaaacatca
    18061 ccctgaacct gaacgatgtc accacgggca accagacgct aaccatcact caggacacct
    18121 ccaaagcgca aacggcgatt aaagactggg tgaatgccta taactcgcta atagatacct
    18181 tcagcagcct gaccaaatac accgccgtag atgcgggagc tgatagccag agttctagca
    18241 atggcgcact gctcggcgac tccacgttgc ggacgattca gacgcagttg aaatcgatgc
    18301 tgagtaatac cgttagttct tccaactata aaacgttggc gcagattggt atcacgaccg
    18361 atcccagcga tggcaaactg gaactggatg ccgacaaact caccgctgca ctgaaaaaag
    18421 atgccagcgg cgtaggtgca ttgattgttg gcgatggtaa aaaaaccggc atcacgacca
    18481 ccatcggcag caacctgacc agttggcttt cgacaacagg cattattaaa gccgctaccg
    18541 atggcgttag taagaccctg aataagttaa ccaaagacta caacgccgcc agcgatcgca
    18601 ttgatgcgca ggtcgcgcgc tacaaagagc aatttaccca actggacgtt ttaatgacct
    18661 cgttaaacag caccagcagc tacttaacgc agcagttcga aaacaacagt aattccaagt
    18721 aatcaatatt catcgggaga caggtcatgt acgcggcaaa aggcacccag gcctatgcac
    18781 aaattggcgt cgaaagcgcc gtaatgagcg ccagccagca acagctggtc accatgctat
    18841 ttgatggagt gctgagcgca ctggttagag cgcgcctgtt tatgcaggac aacaatcagc
    18901 aaggcaaagg cgtctctttg tcaaaagcga tcaacatcat tgagaacggg ctgcgggtga
    18961 gtcttgatga agagagcaaa gacgaactaa cccaaaactt gattgctctt tatagctata
    19021 tggtcaggcg cttgctgcaa gccaatttac gcaacgatgt ctccgcagtc gaagaagtgg
    19081 aagcattaat gcgcaatatt gccgatgcct ggaaagagtc tttactctcc ccttctttga
    19141 ttcaggaccc agactgatga acaatgcacc gcatttgtat ttcgcctggc aacaactcgt
    19201 cgaaaaaagt cagctcatgt tacgcctggc gacagaagaa caatgggacg aactcatcgc
    19261 cagcgaaatg gcgtatgtga atgcggtgca ggagatcgca cacctgactg aagaggttgc
    19321 cccgtccacc acgatgcagg agcagctccg cccgatgctg cgcctgattc tcgacaacga
    19381 aagcaaggta aagcagttat tacagattcg gatggatgaa ctggcgaaac tggtcggtca
    19441 gtcatcggtg caaaaatcgg tgttaagtgc ctatggcgat cagggtggct ttgtgctggc
    19501 tccgcaggat aacctctttt gaatcttaaa gagccaatgg ctcgcgaata atctgattac
    19561 ggctacgctt ctaatgttcc ccttgaatgg agtcgaagaa tgcgtaatcc cacgctgtta
    19621 caatgttttc actggtatta cccggaaggc ggtaagctct ggcctgaact ggccgagcgc
    19681 gccgacggtt ttaatgatat tggtatcaat atggtctggt tgccgcccgc ctataaaggc
    19741 gcatcgggcg ggtactcggt cggctacgac tcctatgatt tatttgattt aggcgagttt
    19801 gatcagaaag gcagcatccc tactaaatat ggcgataaag cacaactgct ggccgccatt
    19861 gacgccctga aacgtaatga cattgcggtg ctgctggacg tggtagtcaa ccacaaaatg
    19921 ggcgcggatg aaaaagaagc tattcgcgtg caacgtgtaa atgctgatga ccgtacgcaa
    19981 attgacgaag aaatcattga gtgcgaaggc tggacgcgtt acaccttccc cgcgcgtgcc
    20041 gggcaatact cgcagtttat ctgggatttc aaatgtttta gcggtatcga ccatatcgaa
    20101 aaccctgatg aagatggcat ttttaaaatt gttaacgact ataccggaga aggctggaac
    20161 gatcaggttg atgatgaact cggtaatttc gattatctga tgggtgagaa tatcgatttt
    20221 cgcaatcatg ccgtgacgga agagattaaa tactgggcgc gctgggtgat ggaacaaacg
    20281 caatgcgacg gctttcgtct tgatgcggtc aaacatattc cggcatggtt ttataaagag
    20341 tggatcgaac acgtacagga agttgcgcca aagccgctgt ttattgtggc tgaatactgg
    20401 tcgcatgaag tcgataaact gcaaacgtat attgatcagg tggaaggcaa aaccatgctg
    20461 tttgatgcgc cgctgcagat gaaattccat gaagcatcgc gcatgggtcg cgactacgac
    20521 atgacgcaga tcttcactgg gacgctggtg gaagccgatc cttttcacgc cgtaaccctc
    20581 gttgccaacc acgacaccca accgttgcaa gccctcgaag caccggtcga accgtggttt
    20641 aaaccgctgg cgtatgcctt aattttgttg cgggaaaatg gcgttccttc ggtgttctat
    20701 ccggacctct acggtgcaca ttacgaagat gtcggtggtg acgggcaaac ctatccgata
    20761 gatatgccaa taatcgaaca gcttgatgag ttaattctcg cccgtcagcg tttcgcccac
    20821 ggtgtacaga cgttattttt cgaccatccg aactgcattg cctttagccg cagtggcacc
    20881 gacgaatatc ccggctgcgt ggtggtcatg tcgaacgggg atgatggcga aaaaaccatt
    20941 catctgggag agaattacgg caataaaacc tggcgtgatt ttctggggaa tcggcaagag
    21001 agcgtagtga ccgacgaaaa cggcgaagcg accttctttt gcaacggcgg cagcgtcagc
    21061 gtgtgggtta tcgaagaggt gatttaaatt catccccggc ggcaagccgg ggagatttca
    21121 ttacggcagt ggcgtcggta acggcgcttt atccagcgcg gcggcacact ctgccgtcgg
    21181 gcgatccaca cgctccatcg tcataccatc gtactcaatg gtgttaccgt cacgctcaac
    21241 ctcgtacagc tcacgtttca cggtaacgtt agtgagatca tcagacatca atgtcaactt
    21301 acccggcacg gcaatcaccc gctgccactg acggcaatcc agggtatcgc cctctttagt
    21361 gacaatcagg ctgcctatcg cttccggact gaccagcgca ctttgcggcc ctttggtctg
    21421 ccagtagccc gccagccagt ccggcgcggg ggttttcacg acattgttat agttttcgac
    21481 ttcggcacag cccgccagca acatgagcgc gcctgcaatt gcgagttttt tcatcattcg
    21541 tcctgcatga gaagaaaaga ggattgtggc attaaagccc tgatgtcgcc agcctttcca
    21601 ggttcagaga gaaatttgat ctaactatta tttttgtgaa ttattacccc gaaaataaat
    21661 ttgcttgatg tatgattcgc aactcttgtt tcaaaccttc tgagttcaga ggctacatac
    21721 atgtcatggc agcaattcaa acacgcctgg ttgattaaat tctgggcccc catccctgcg
    21781 gtcatcgcgg cgggtattct ctccacttac tactttggca tcactggcac cttttgggct
    21841 gtcacgggtg aatttacccg ctggggcggt cagcttctgc agctgttcgg cgtccatgct
    21901 gaagagtggg gttattttaa aattatccat ctggaaggat cgccattaac ccgcatcgac
    21961 ggcatgatga tcctcggtat gtttggcggc tgctttgctg cagcgctgtg ggccaacaat
    22021 gtcaaactac gcatgccacg cagccgtatc cgcattatgc aggccattat tggcggcatt
    22081 atcgctggtt ttggcgcgcg tctggcaatg ggctgtaacc tggcggcgtt ctttaccggc
    22141 attcctcagt tctcgctgca tgcctggttc tttgccatcg ccactgccat tggttcatgg
    22201 tttggcgcgc gctttaccct gctgcccatc ttccgtattc ccgtgaaaat gcagaaagtt
    22261 tctgccgcct caccgctgac gcaaaaaccg gatcaggcgc ggcgtcgttt tcgtctcggg
    22321 atgctggtct ttttcggcat gctgggctgg gcgctgctca cggcgatgaa ccaaccaaaa
    22381 ctggggctgg caatgctgtt tggcgtcggc tttggtttac tgattgaacg tgcgcaaatc
    22441 tgctttactt cggcgttccg cgatatgtgg atcaccggac gtacccatat ggcgaaagca
    22501 atcattatcg gtatggcggt gagtgccatc gggatcttca gttacgtaca gttaggcgtt
    22561 gaacctaaaa tcatgtgggc gggaccaaat gcggtaattg gtggtttact ttttggtttt
    22621 ggcatcgtgc tggcaggcgg ctgcgaaacc ggctggatgt accgcgcggt agaaggccag
    22681 gtgcactact ggtgggtcgg tctgggcaac gtgatcggct caacgattct ggcgtattac
    22741 tgggatgatt tcgctccggc gctggccacc gactgggaca aaatcaacct gctgaaaacc
    22801 tttggtccga tgggtggcct gctggtgaca tatttgctgt tgtttgcagc tctaatgttg
    22861 attatcggct gggaaaaacg cttcttccgc cgtgcggcac cgcagattgc taaggagatc
    22921 gcatgaaaaa tatcgttcct gattaccgtc ttgatatggt gggtgaaccc tgcccttatc
    22981 cggctgtcgc aacccttgag gcgatgccgc agttaaaaaa aggggagatc ctggaagtgg
    23041 tgagcgactg tccgcagtcg atcaataata ttccactgga tgcgcgtaat cacggctata
    23101 cggtgctgga tattcagcaa gacgggccga ccattcgtta tttaattcag aagtaatccc
    23161 tcattcccgc tgtggcccac agcgggaaca tcgttaccac tctatcacat agattttacc
    23221 cgccccctct cctattcttt tcagtgtgct ctcaggaggt ttctatgtgt ggacgctttg
    23281 cccaatccca aacgcgtgaa gattacctcg cgtttctcgc ggaagatatt gaacgcgata
    23341 ttccctacga tcccgaacct attggcagat acaacgtcgc gccgggaacc aaagtccttc
    23401 tgctcagtga acgtgatgaa caccttcatc tggatccggt tttctgggga tatgctcccg
    23461 gatggtggga taaaccgccg ctgattaacg cccgcgtaga aactgccgca accagtcgca
    23521 tgtttaaacc gctctggcaa cacggtcggg caatctgttt tgccgatggc tggtttgagt
    23581 ggaaaaaaga aggcgacaaa aaacagcctt attttatcta tcgcgctgac ggacaacctg
    23641 tttttatggc cgcgataggt agcacaccgt ttgagcgtgg tgacgaagcc gaaggatttt
    23701 tgatcgtcac tgcggcggca gatcaaggtc tggtagatat tcatgaccgc cgcccgctgg
    23761 ttctgtcgcc agaagctgta cgggaatgga tgcggcaaga ggttggcggt aaagaagcct
    23821 cagaaattgc tgccagtggc tgtgtgccag caaaccagtt cacctggcac ccggtatcgt
    23881 gcgcggtggg taatgttaaa aaccagggag cggcgttaat tcaacctgtt tgataagcct
    23941 ggagattatt gatcaacaat actgcgtcat aagaaatctc tattagacaa agatttcagt
    24001 acctgttggc atattgcaaa aataacacca atacggaatc gtcatgttca cgattaaaac
    24061 agatgatctc acccatccag cagtgcaagc attagtggct taccatattt ccggcatgct
    24121 gcagcagtct ccccccgaaa gtagtcatgc tttagatgtg caaaaattac gtaacccgaa
    24181 agtgacattc tggtcagtat gggaaggcga acaactcgca ggaattggtg cgctgaagtt
    24241 gctggatgat aaacatggcg aactgaaatc aatgcgtacc gcgccaaatt atttacgtcg
    24301 gggtgtcgcc agtctgattt tacgccacat tttgcaggtc gcccatgaca gatgccttca
    24361 tcgcctgagc ttagaaacgg gtacacaggc tggatttacg gcctgccatc aactttattt
    24421 gaaacatgga ttcgttgatt gcgaaccgtt tgctgattat caacttgatc cgcacagtcg
    24481 atttttgtca ttgacgctat gcgaaaataa tgaattgcca tgagccagac gcagcacatt
    24541 cttgcattcg acgtgctgcg cctttattta ttatcagccg caaatgtctt gtccttagac
    24601 gctctttcca catgcgccac aagaaacctc tattccagtg acacaattat gcctgattat
    24661 ttccctagcc cggaatatga ataaaaacat caaataagat gaaattatca aaatattaat
    24721 gttgcgtctg aggtcgtaac aacatccgac gtggtgatat gtcaaatgag acactctgat
    24781 ggtcaaaggg tttatcgcgt ttacagatcg tggatggcag aaaactttca gaatcaggtt
    24841 tccattttaa accagaaatt aagtgagttt gtcccatcca tgccccacgc ggtgagatcg
    24901 gatgtaatca acaacaggtt acaaaaccta cacctgcatg ctcatcactt cctgatacgc
    24961 cgccaccagc ttattacgca cctgaatccc catttgcatt gaaactgagg ctttttgcat
    25021 atcggtcatc acatcgttta acgccacgcc gggttcaccg agagtgaatt tttctgcctg
    25081 cgtgcgggcg gctgtttgtg tatcgcttat gcgatcgagc gcggcgtgca gctgcccggc
    25141 aaaactaatg gtcggttgtg gcagtgattc ctgtgtacgc gcactcatcg ccgtagcctg
    25201 taactggctg ataacccctt caatcccctg tatcgctgac attttcatct cccggataat
    25261 ttctggcagc aaagcctacc agtaagtcaa taagacaaag gcgctaaata gcaacaaaaa
    25321 aacgggttta ttggcggata gaaaaaaacg aaagcacaaa taatgggagc gtcaattttt
    25381 cgagtttgct gacccgggag tgagtcttgt tccactttgc caataacgcc gtccataatc
    25441 agccacgagg tgcgcgatga atgcgactgc agcccagaca aaatctcttg agtggcttaa
    25501 tcgcctgcgt gcgaatccga aaattccatt gattgttgcc ggctccgcag cagtggcggt
    25561 catggtcgca ctgatcctgt gggcgaaagc ccccgactac cgcacattat tcagcaatct
    25621 ttccgatcag gatggtggcg caattgtcag ccaactgacg caaatgaata ttccttaccg
    25681 cttcagcgaa gccagcggcg ctattgaagt tccggcagat aaagttcacg aactgcgcct
    25741 gcgcctggcg caacaaggtt tgcccaaagg cggcgcggtc ggtttcgaac tgctcgatca
    25801 ggaaaagttt ggtatcagcc agttcagcga acaggtgaat tatcagcggg cgctggaagg
    25861 cgagctttct cgtaccatcg aaactatcgg cccggtaaaa ggggcgcgcg tacatctggc
    25921 aatgccgaaa ccgtctttat tcgtccgtga acaaaaatcc ccttctgcat cggtgacggt
    25981 aaatctgtta cccggccgcg cactcgatga agggcaaatt agcgccattg tacatctggt
    26041 ttccagcgcg gttgctggtc tgccgccggg gaacgtcacg ctggtggatc agggtggaca
    26101 tctgttaacc cagtccaata ccagcgggcg cgatcttaat gacgctcagt tgaaatatgc
    26161 cagcgatgtc gaaggccgga ttcagcggcg tattgaagcg atcctgtcgc caattgttgg
    26221 taacggtaat attcacgccc aggtcacggc gcaactggac ttcgccagta aagaacaaac
    26281 agaagaacag tatcgcccta acggtgatga atctcaggcg gcgcttcgtt cacgccagct
    26341 taatgagagc gagcaaagcg gttccggtta tccaggcggc gtaccggggg cgttgtcgaa
    26401 tcaaccggca cctgcgaata acgcgccaat cagcacgcct ccggcaaatc aaaataaccg
    26461 ccagcagcag gcgagcacca ccagcaatag tgggccgcgt agcacacagc gtaatgaaac
    26521 cagtaactac gaagtcgatc gcaccattcg tcataccaaa atgaacgtgg gcgatgtgca
    26581 acgtctgtca gtcgcggtag tggtgaatta caaaaccttg ccagacggta aaccgttgcc
    26641 tctcagcaac gaacagatga agcaaattga agatctgacc cgcgaggcga tgggcttttc
    26701 tgaaaaacgc ggtgactcgc tcaatgtcgt taactcgccg ttcaatagta gtgaggaaag
    26761 cggcggagaa ctgccgttct ggcaacaaca agcgtttatc gatcagttac ttgctgccgg
    26821 tcgctggttg ctggtactgc tggtggcgtg gctgctgtgg cgtaaagcgg tacgcccgca
    26881 gctaacacgt cgcgctgagg cgatgaaagc tgtacagcaa caggcgcagg cccgcgagga
    26941 agtggaagat gcggtggaag tccgcctgag caaagacgaa caactccaac aacggcgcgc
    27001 taaccaacgt ctgggggcag aagtcatgag ccagcgtatc cgtgaaatgt ctgataacga
    27061 tccgcgcgtg gtggcgctgg tcattcgcca gtggataaat aacgatcatg agtaacctga
    27121 caggcaccga taaaagcgtc atcctgctga tgaccattgg cgaagaccgg gcggcagagg
    27181 tgttcaagca cctctcccag cgcgaagtgc aaactctgag cgctgcaatg gcgaacgtca
    27241 cgcagatctc caacaagcag ctaaccgatg tgctggcgga gtttgagcaa gaagctgaac
    27301 agtttgccgc actgaatatc aacgccaacg attatctgcg ttcggtattg gtcaaagctc
    27361 tgggtgaaga acgtgccgcc agcctgctgg aggatattct cgaaacccgc gataccgcca
    27421 gcggtattga aacgctcaac tttatggagc cgcagagcgc cgccgatctg attcgcgatg
    27481 agcatccgca aattatcgcc accattctgg tgcatctgaa gcgcgcccag gccgccgata
    27541 ttctggcgtt gttcgatgaa cgtctgcgcc acgacgtgat gttgcgtatc gccacctttg
    27601 gcggcgtgca gccagccgcg ctggcggaac tgaccgaagt actgaatggc ttgctcgacg
    27661 gtcagaatct caagcgcagc aaaatgggcg gcgtgagaac ggcagccgaa attatcaacc
    27721 tgatgaaaac gcagcaggaa gaagccgtta ttaccgccgt gcgtgaattc gacggcgagc
    27781 tggcgcagaa aatcatcgat gagatgttcc tgttcgagaa tctggtggat gtcgatgatc
    27841 gcagcattca gcgtctgttg caggaagtgg attccgaatc gctgctgatc gcgctgaaag
    27901 gggccgagca gccactgcgc gagaaattcc tgcgcaatat gtcgcagcgt gccgccgata
    27961 ttctgcgcga cgatctcgcc aaccgtggtc cggtgcgtct gtcgcaggtg gaaaacgaac
    28021 agaaagcgat tctgctgatt gtgcgccgcc ttgccgaaac cggcgagatg gtgattggca
    28081 gcggcgagga tacctatgtc tgataatctg ccgtggaaaa cctggacgcc ggacgatctc
    28141 gcgccaccac aggcagagtt tgtgccaatg gtcgagtcgg aagaaaccat tattgaagag
    28201 gccgaaccca gccttgagca gcaactggcg caactgcaaa tgcaggccca tgagcaaggt
    28261 tatcaggcgg gtgttgccga aggtcgccag caaggtcata agcagggcta tcaggaagga
    28321 ctggcccagg ggctggaaca aggtctggca gaggcgaagt ctcaacaagc gccaattcat
    28381 gcccggatgc agcaactggt cagcgaattt caaactaccc ttgatgcgct tgatagtgtg
    28441 attgcttcgc gcctgatgca gatggcgctg gaggcggcac gtcaggtgat cggtcagacg
    28501 ccaacggtgg ataactcggc actgatcaaa cagatccaac agttgttgca gcaagaaccg
    28561 ttattcagcg gtaaaccaca gctgcgcgtg cacccggatg atctgcaacg tgttgatgat
    28621 atgctcggcg caactttaag tttgcatggc tggcgcttgc ggggcgatcc caccctccat
    28681 cctggcggct gtaaagtctc cgccgatgaa ggcgatctcg acgccagtgt cgccactcgc
    28741 tggcaagaac tctgccgtct ggcagcacca ggagtggtgt aatgaccacg cgcctgactc
    28801 gctggctaac cacgctggat aactttgaag ccaaaatggc gcagttgcct gcggtacgtc
    28861 gctacgggcg attaacccgc gctaccgggc tggtgctgga agccaccgga ttacaattgc
    28921 cgctcggcgc aacctgtgtc attgagcgcc agaacggcag cgaaacgcac gaagtagaaa
    28981 gcgaagtcgt tggctttaac ggtcaacggc tgtttttaat gccgctggag gaagtcgaag
    29041 gtgtcctgcc cggcgcgcgt gtttatgcca aaaacatttc ggctgaaggg ctgcaaagcg
    29101 gcaagcagtt gccgctcggt tcggcgttat taggtcgcgt tctggacggc agcggtaaac
    29161 cgcttgatgg cctcccctcc cccgatacga cggaaaccgg tgcgctgatt accccgccat
    29221 ttaacccgtt gcaacgtaca ccgattgaac atgtgctgga caccggcgtg cgcccaatca
    29281 atgccctgct taccgtcggg cgcgggcagc gtatggggct gtttgccggg tccggcgttg
    29341 gtaaaagtgt gctgctgggg atgatggcac gttacacccg cgccgatgtc attgtcgtgg
    29401 gtttgattgg tgaacgaggg cgcgaagtaa aagattttat tgagaatatc ctcggtgccg
    29461 aaggacgtgc acgctccgtg gtgattgccg ctccggcgga tgtttctccg ctcctgcgaa
    29521 tgcagggtgc cgcctatgcc acgcgcattg ccgaagattt tcgcgatcgt ggtcagcatg
    29581 tgttgctgat tatggactcc ctcacccgct acgcgatggc ccagcgtgaa attgcgctgg
    29641 cgattggcga accacctgcc actaaaggtt atccaccgtc ggtgtttgcc aaattaccgg
    29701 cactggtcga gcgtgccgga aatggcatta gcggcggcgg ctcgattacc gcgttttata
    29761 ccgtgctcac cgaaggcgat gaccagcagg accccattgc cgactccgcg cgggcaatcc
    29821 tcgacggtca cattgtgctg tctcgccgac tggcggaagc cgggcactat ccggctatcg
    29881 atattgaagc gtcgatcagt cgcgcaatga cggcgttgat cagtgagcaa cattacgcgc
    29941 gagtgcgcac cttcaaacag ctgttgtcga gttttcagcg taaccgcgat ctggttagcg
    30001 tcggcgcgta tgccaaaggc agcgatccga tgctcgacaa agccatcgcc ctgtggccgc
    30061 agctggaggg ctatttgcaa caaggcattt ttgaacgcgc ggactgggaa gcgtctctcc
    30121 aggggctgga gcgtattttc ccgacagtat cataacccag gagataacgg cagatgacag
    30181 aacatggcgc gctggcgacc ctgaaagatc tggcagaaaa agaggtagag gatgccgcgc
    30241 gcctgctggg tgaaatgcgt cggggatgtc agcaggcgga agaacagctc aaaatgctga
    30301 tcgattatca gaatgaatat cgcaataacc tcaatagcga tatgagtgcc gggatgacca
    30361 gcaaccgctg gatcaactat cagcagttta tccagacgct ggaaaaagcc attactcagc
    30421 atcgccagca acttaatcag tggacgcaga aagtagacat tgccctgaac agctggcgag
    30481 aaaagaaaca acgtttgcag gcttggcaga cactgcagga acggcaatcc acggcggcac
    30541 tgcttgcaga aaaccgcctc gatcagaaaa agatggatga gttcgcccag cgcgccgcca
    30601 tgaggaaacc tgaatgattc gcatagcgcc cttgattacc gctgacgttg ataacgccac
    30661 attgcctggc ggcaaagcca gcgatgctgc acaagatttt ctcgcgttgt tgagcgaagc
    30721 attagcaggc gagacaacaa ccgacaaagc ggccccccag ttgctggtgg caacagataa
    30781 gcccacgaca aaaggcgagc cgctggtcag cgatattgtt tccgatgcgc aacaagcaga
    30841 tttactgatc cctgtggatg aaacaccgcc tgtcatcaac gacaaacaat ccacatcaac
    30901 atcgttaacc accgctcaga cgataacgtt ggctgcggcg gctgacaaca atacggcaaa
    30961 agacgaaaaa gcggatgatc tgaatgaaga cgtcaccgca agcctgagcg ccctttttgc
    31021 gatgttgccg ggttttgaca atacgcccaa agtgactgat gcaccgtcaa ccgtgttacc
    31081 gacagagaaa ccaacgcttt tcacaaaact gacttctgcg caactcacta cagcacagcc
    31141 tgatgacgcc cccggcacac cagctcagcc attaacaccg ctggtagcag aagcccagag
    31201 taaagcggaa gtcatcagca cgccttcgtc ggtgaccgct gccgccagcc cgctaatcac
    31261 tccacaccag acacagccac tgcccaccgt cgccgcgcct gttttgagtg caccgctggg
    31321 ttctcacgaa tggcaacaat cattaagcca gcatatttcg ctgttcaccc gccaggggca
    31381 acaaagtgca gagttgcgtc tgcacccaca ggatttaggt gaagtgcaaa tttccctcaa
    31441 agtggatgat aaccaggcgc aaatccagat ggtttcaccg catcagcacg tacgcgccgc
    31501 cctggaagca gcgctgccgg tactgcgtac gcagctggcc gaaagtggca ttcagttagg
    31561 gcaaagcaac atcagtggcg aaagctttag tggtcagcag caggccgctt cccaacaaca
    31621 gcaaagccaa cgcacagcaa accatgaacc tctggcgggg gaagacgacg atacgcttcc
    31681 ggttcccgtc tctttacaag ggcgtgtaac aggcaacagc ggcgttgata ttttcgccta
    31741 acgtcagagg tagcaccgta atccgcgtct tttccccgct ttgttgcgct caagacgcag
    31801 gataattagc cgataagcag tagcgacaca ggaagaccgc aacacatgac tgattacgcg
    31861 ataagcaaga aaagcaagcg atcgctttgg atcccgattc tggtattcat taccctcgcg
    31921 gcctgtgcca gcgcaggtta cagctactgg cattcgcatc aggttgccgc tgaagacaaa
    31981 gcgcagcaac gcgtcgtgcc ctccccggtc ttctatgcgc tggatacctt tacggtcaat
    32041 ctgggcgatg cggatcgcgt actttatatc ggcataaccc tgcgactcaa agatgaagct
    32101 acccgctcgc ggctgagtga gtatttgccg gaagtccgta gccgtttgct gttactgttt
    32161 tcgcgtcagg atgctgccgt actggcgaca gaagaaggta agaaaaacct gattgccgag
    32221 attaaaacca cactttccac cccgcttgtt gccgggcaac cgaaacagga tgtcaccgac
    32281 gtgctgtata ccgcttttat tctgcgataa cgacatgggc gatagtattc tttctcaagc
    32341 tgaaattgat gcgctgttga atggtgacag cgaagtcaaa gacgaaccga cagccagtgt
    32401 tagcggcgaa agtgacattc gtccgtacga tccgaatacc caacgacggg ttgtgcgcga
    32461 acgtttgcag gcgctggaaa tcattaatga gcgctttgcc cgccattttc gtatggggct
    32521 gttcaacctg ctgcgccgta gcccggacat taccgtcggg gccatccgca ttcagccgta
    32581 ccatgaattt gcccgcaact tgccggtgcc gaccaacctg aaccttatcc accttaaacc
    32641 gctgcgcggc acagggctgg tggtgttctc accgagtctg gtgtttatcg ccgtggataa
    32701 cctgtttggc ggcgatggac gcttcccgac caaagtggaa ggtcgcgagt ttacccatac
    32761 agaacagcgc gtcatcaacc gcatgttaaa gctggcgctt gaaggctata gcgacgcctg
    32821 gaaggcgatt aatccgctgg aagttgagta cgtacgttcg gaaatgcagg tgaaatttac
    32881 caatatcacc acctcaccga acgacatcgt ggttaacacg ccgttccatg tggagattgg
    32941 caacctgacc ggcgaattta atatctgtct gccattcagc atgatcgagc cgctacggga
    33001 attgttggtt aacccgccgc tggaaaactc gcgtaatgaa gatcagaact ggcgcgataa
    33061 cctggtgcgc caggtgcagc attcacagct ggagctggtc gccaactttg ccgatatatc
    33121 gctacgcctg tcgcagattt taaaactgaa acccggtgac gtcctgccga tagaaaaacc
    33181 cgaccgcatc atcgcccatg ttgacggtgt cccggtgctg accagtcagt acggcaccct
    33241 caacggtcag tatgcgttac ggatagaaca tttgattaac ccgattttaa attctctgaa
    33301 cgaggaacag cccaaatgag tgacatgaat aatccggccg atgacaacaa cggcgcaatg
    33361 gacgatctgt gggctgaagc gttgagcgaa caaaaatcaa ccagcggcaa aagcgctgcc
    33421 gacgcggtgt tccagcaatt tggcggtggt gatgtcagcg gaacgttgca ggatatcgac
    33481 ctgattatgg atattccggt caagctgacc gtcgagctgg gccgcacacg aatgaccatc
    33541 aaagagttat tgcgtctgac gcaagggtcc gtcgtggcgc tggacggcct ggcgggcgaa
    33601 ccgctggata ttctgatcaa cggttattta attgctcagg gcgaagttgt cgtagtcgct
    33661 gataaatatg gcgtgcggat caccgatatc attactccgt ctgagcgaat gcgtcgcctg
    33721 agccgttagt gatgaataac catgctactg tgcaatcttc cgcgccggtt tctactgcgc
    33781 cactgctgca ggtgagcggc gcactcatcg ccattattgc cctgatcctc gctgctgcct
    33841 ggctggtaaa acggctggga tttgccccga aacgcactgg cgttaacggt ctgaaaatta
    33901 gcgccagtgc ttcgctgggc gcgcgtgaac gggtcgtggt ggtcgatgtg gaagatgcac
    33961 ggctggtgct cggcgttacc gcagggcaaa tcaatctgct gcataaactt cccccttcag
    34021 caccaacgga agagataccg cagaccgatt ttcagtcggt catgaaaaac ttgcttaagc
    34081 gtagcgggag atcctgatgc gtcgtttgtt gtctgtcgca cctgtccttc tctggctggt
    34141 tacacccctc gccttcgcgc aactgacggg tattaccagc cagccgctgc ctggcggtgg
    34201 acaaagctgg tcgctcccgg tgcagacgct ggtgttcatt acctcgttga cgtttattcc
    34261 ggcaatttta ctgatgatga ccagtttcac ccgcatcatc attgtttttg gtttattgcg
    34321 taacgcactg ggaacaccct ccgcgccacc taaccaggta ttgctggggc tggcactgtt
    34381 tttgaccttt tttattatgt caccggtgat cgataaaatt tatgtcgatg cgtaccagcc
    34441 attcagcgaa gagaaaatct caatgcagga ggcgctggaa aaaggggcgc agccgctgcg
    34501 tgagtttatg ctgcgtcaga cccgtgaggc ggatttagga ttgtttgcca gactggcaaa
    34561 tactggcccg ttgcagggac ctgaagccgt gccgatgcgc attttgctcc cggcttacgt
    34621 gaccagcgag ttgaaaaccg catttcagat aggcttcacg atcttcattc cgtttttgat
    34681 tatcgacctg gtgattgcca gcgtgttgat ggcattgggg atgatgatgg tcccccccgc
    34741 caccattgct ctgcccttta aactgatgct gtttgtactg gtggatggct ggcaattgct
    34801 ggtcggttcg ctggcgcaga gcttttacag ctagagaggc aaaatgacac ctgaatcggt
    34861 catgatgatg gggactgaag cgatgaaagt cgcgctggca ctggctgccc cgctattgct
    34921 ggtagcattg gtcacgggcc ttatcatcag tattttgcag gccgccacgc agattaacga
    34981 aatgacgctg tcgtttattc cgaaaatcat cgccgtattt atcgccatta ttattgccgg
    35041 accgtggatg ctcaatctgt tgctggatta cgtccgcacc ttgttcacta acctgccgta
    35101 tatcatcggg taggccgtac aatgatgcag gtgacaagcg atcaatggct ttcctggcta
    35161 agtctgtact tctggccgtt actgcgcgtg ttggcgctga tctccaccgc gccgattctg
    35221 agcgaacgca gcgtaccgaa acgggtaaaa ctgggtctgg caatgatgat cacgttcgct
    35281 attgccccat cattacctgc caacgatgtt cctgtttttt cgttctttgc tctgtggctg
    35341 gccgtgcagc agatcctgat cggcattgcg cttggtttta ccatgcaatt tgcctttgcc
    35401 gcggtgcgaa ccgctggtga aattatcggt ctgcaaatgg ggctgtcatt tgcgacgttt
    35461 gtcgatccgg ccagccatct taatatgccc gttttagcgc gtatcatgga tatgctggcg
    35521 ttactgctgt tcctgacatt taacggtcat ttatggttga tttcactgct ggtcgatacc
    35581 tttcacaccc tgccaattgg tggcgaaccg ttgaacagca atgcgtttct ggcactcacc
    35641 aaagcaggga gtttgatttt ccttaacggg ctgatgctgg cgttaccgct cattactctg
    35701 ctgctgaccc tgaatctggc attaggttta ctaaatcgta tggccccgca attatccatt
    35761 tttgttattg gatttccatt aactctgact gtcggtatct ctttaatggc ggcattaatg
    35821 ccgttaattg cgcctttttg cgaacattta ttcagtgaga tgtttaattt gctggctgat
    35881 attattagtg aattgccatt aatataattc cgtaacgttt atcatgctac cctaaggatt
    35941 atccgaaaaa taatacctac gaacatcttc caggatactc ctgcggcgaa atatttgttt
    36001 taagctcact cacatatcgc aacatttact ttactttaag acaattccag gcaaattata
    36061 caacacttta cgggatagta agtccgcctg aaaaatcgcg agagtggcgc attaggtgac
    36121 ccatgttgtt ccgtttagtc atgatgaaat attcaggtaa ggggaattat cgttacgcat
    36181 tgagtgaggg tatgccatgt caacgattat tatggattta tgtagttaca cccgactagg
    36241 tttaaccggg tatctgttga gtagaggggt taaaaaaaga gaaatcaacg acattgaaac
    36301 cgttgatgac cttgccatag cttgtgattc acagcgccct tcagtggtgt ttattaatga
    36361 ggactgtttc atccacgatg cttctaacag tcagcgcatc aagcacatca ttaatcaaca
    36421 tcccaatacg ttatttatcg tttttatggc aattgccaat gttcattttg atgaatatct
    36481 attggtcaga aaaaatttat tgatcagttc taaatcgatt aaaccggaat ctctcgatga
    36541 tatccttggc gatattctga aaaaagagac aacgataacc tcgtttttaa atatgccgac
    36601 gttatcattg agccgaaccg aatcgagtat gttgcgaatg tggatggcag gtcagggaac
    36661 cattcaaatc tccgaccaaa tgaatatcaa agccaagacc gtttcatcgc ataaaggtaa
    36721 tattaaacgt aagatcaaaa cgcataataa acaggttatc taccatgtcg tccgactgac
    36781 ggataatgtg actaatggta tttttgtcaa catgcgctaa cacattctga ctggtggttt
    36841 cccaccagtc aggctgaatt agattactct gctttctcca caaagatacc gtcctgatgc
    36901 cctgcttcat taaagaacca gatgccgagc gggtagtctt ccagcgaaac caggtacatt
    36961 gtgccttcac taaactcctc aactgccagt accacgccag gacgacgcgg accgccatcc
    37021 gttttgactg ttacccgatc attcaccttc atcgttttcc tcctgtggct ttgtgccagt
    37081 gtagaacaat ttcgtttttc tggcagcgcc aggcgcgcgc gagtgctgat tttctcgacg
    37141 gtctatactt aagagatgcc aggcggactt aacgactggc ggcaacaaca gagtaacggt
    37201 tgcgaggaaa gatgatgaaa accgcaaaag agtacagcga taccgcaaaa cgtgaggtca
    37261 gcgtcgatgt cgatgccctg ctggcggcga tcaatgaaat tagcgaaagc gaagttcatc
    37321 gcagccagaa cgattctgaa cacgttagtg tcgatggacg tgaatatcat acatggcgtg
    37381 aattggcgga tgccttcgaa ctggatattc atgacttcag cgtctctgaa gtgaatcgtt
    37441 gaatgcacaa taaaaaaaat cccgaccctg agggggtcgg gatgaaactt gcttaagcaa
    37501 gaagcactta aaaaattcgt tacaccagga aatctgatgt gttcatcacc ttatccgcaa
    37561 tttttttcgc tgacaagaaa atattcgtga tatgaatgat taacgctact tatgaataga
    37621 aatgtgacgc gcgtcacccc ttggtggctc tggcgtcaac gggatcgctc ccaaaaaaat
    37681 catctttgat aatttgtgct gttttgagaa tcatcctggt aaggggagta ctgcgcaacc
    37741 atgctttcaa ttcaacaacc actactggtt tttagcgatc ttgatggcac cctgctggac
    37801 agtcatagtt atgactggca accggcagcc ccctggctca gccgtttacg cgaagcgaat
    37861 gttcccgtca ttctctgtag cagtaaaact tcagcggaaa tgctgtactt gcaaaaaatg
    37921 ttggggctac aaggcttacc gctgattgca gagaatggcg cagtgatcca gcttgctaag
    37981 caatggcagg agatagacgg ttttccacgc atcatctcag gtattagcca tggcgaaatc
    38041 agccaggttt taaatacgct acgcgagaaa gaacatttta aattcacgac ttttgatgat
    38101 gtcgacgatg caaccatcgc cgaatggacg ggattaagcc gtagccaggc ggcgctgacg
    38161 cagctgcatg aggcgtcggt aacgctaatc tggcgcgaca gtgacgagcg tatggcacaa
    38221 tttaccgctc gtctgaacga actgggcttg cagtttatgc agggtgcgcg cttctggcac
    38281 gtcctggatg cctctgccgg aaaagatcag gctgccaact ggattatcgc gacctatcaa
    38341 caattgtcag gcaaacgccc aaccacactt ggcctgggcg atgggccaaa cgatgcgccc
    38401 ttactggagg taatggatta cgcggtgatt gtgaaagggc taaaccgtga aggggtccat
    38461 ctgcatgatg aggatccggc ccgcgtctgg cgaacgcagc gtgaaggacc ggaaggctgg
    38521 cgtgaagggc tggaccattt tttctccgcc cgttaagcgt tatcgctcgc gaatacccga
    38581 ttgcgcccgg cctgttttgc gaggtaaagc cgacggtcgg ccaatgactg gagttgttca
    38641 aaatcataat caccggtttc ctcgctgctg cttaccccca gcgaggcact gatgcgtatc
    38701 gtcgtactct tggcgatcag catctctttt tcatttaact tcaggcgaat acgttctgcg
    38761 acctccgcag cctgcgtcag attcgcgcct ggcagaatca cacaaaactc ctcaccaccg
    38821 acccgcccgg caacgtcctg cgcacgtaag gaactgctaa ttaatccggc agcatgagaa
    38881 agaacacggt cgcccacctg atgaccaaag cggtcattaa tcgctttaaa atggtcaaga
    38941 tcgacctgaa tgacagaaaa tggatgttgg tgcgtctgac acaatttagc gagcggacgg
    39001 gctttttcga acagtgcgcc acggttatat aaacgcgtta aggtgtcgtg ccacgcctgc
    39061 cactgcaacg agctttgcag aacatacatg ttgctgacca tccggcgaat cacataccag
    39121 gagagtagta acatggtggt aaagagcgcc cacagcaggg ttaatgcaat gctgatactg
    39181 ccgaaatcac cacgcacgcc ttcgcttagc gtatggacgc gcaccagcac accgtcgaaa
    39241 tggtccagac gttcccagct aacataacga ctgttcatac gaatgccacc ccgcgtgtca
    39301 tgttccattg cctgcgccaa caaggccagt tcacgaggat caaaaatatt ccctgttgga
    39361 tggtcaggat tggaagaggt caaaaatctc agcttactgt catagagctg atactcacca
    39421 tcgaggtttt tatcgatggc gtttcttaaa aattgctgca tggtacgcac gggaatactc
    39481 atccccagca cgccatacca gtagttttca ctgtctaccg gaacactgac ggtaacctgc
    39541 ggttcagtat tgctggcgtg ttccggttgc gaagtgaacc agcgtaccgc gcggtgacga
    39601 ttttctcgtt gcgaatggcc gataaaccag ggttgggtga catagccgta ataacgcgtt
    39661 ggtacattgc gcgtaaacaa ggtcggctgc gtcgaaacgt aaaatccggc acgcgagaca
    39721 tacatcgcct gttcaaccat cgacgaggag ttgtgcgcca gtcgcagcaa gtaaccaact
    39781 tccagtgcag cggtaatttc attgtcgagg ctttcatttt cgcgagacag gagattcccc
    39841 tcgctgacta atgcatccga cacgccattg actggcaggg tgcgtcgtcg gttgagttcg
    39901 atttgccagg cgtgctcgtc gcgatgctgt tcgaactcgg ttacggcatt acgcagagag
    39961 gtgaaatcca gtggcgctac gagagcttcg cgcatgccat tacgcaaaaa gatcagtttg
    40021 tcgacgttat actgcaaatg cttatcgagc gcgttggcta cgttttccag atgattacgc
    40081 tggctggaga tataggcatc ttccagcacc accacttcac gccaggtgag caaggtggaa
    40141 aaaagcaata ccacgataaa gcagagatta acgacatgac caggccccag gcggcgtgcg
    40201 agttttttca accagctctg gttttccatt tttgtctcgt gctgcaccct gacgactccc
    40261 aacctgcttt tttatgattc tggcataacc cggcgataac gtaccgcaaa acagcgataa
    40321 ttttataatg gtggcatgat aagacgcgac tatcattcat tgcaggcatt ggcacaccag
    40381 gccggatgtg atccggcctg gtgttaatga taccctttca aacttcttcc gcagaacgac
    40441 tcccctcccc cggaacaaga tcggtttcat gaaaagcttc ccgcttgacc ccgtaaccgt
    40501 cataccaacg acactcaacc ataccgctgg agtatccagt gacaatcatc cgcgggccgc
    40561 cctctttaac cgtaacttcc tcactaacca taaagctcat actcgcctcc ttttttcgag
    40621 tgaaacttgt tcaccttagt tgaagatggc gaattttgca taatagccag tgcgagtatt
    40681 aatgtgcctg accgcgcatt ttcgctactg ctttcactcc cagcaccacg atgccgccga
    40741 tgataaaacc aagaatcaga tttaaaacag tcggtaatat catcgccacc actgcacttt
    40801 gctgcccggc gaaatgttca atggcgtgat gcagcggcgc aataccatgt accacaatcc
    40861 cgccgccgac gaggaacatc gccagcgtgc cgacaatcga taatgctttc atcagccagg
    40921 gcgcgataat caataaccct ttacctaatg cctgcatcag cgcgctggat ttttccgcca
    40981 gccaataccc caggtcatca atcttaacga taacccctac cagaccgtag acgcccacgg
    41041 tcactaccag cgcgatgcct gaaagcacca gcacctgatt aagcaacggc gcctctgcca
    41101 caatccccag cgtgatggca acgatttccg cagacaaaat aaaatcggta cgaatcgccc
    41161 ctttaatttt gtccttttca aacttcagcg gatcctgcgc cgccagcttc tccagacgct
    41221 gctgactctg cgccggatct tctttatgtt tacgcgcctc cagcatatgc agcactttct
    41281 ctaccccttc aaagcagaga aacgcgccgc caatcatcaa cagtggcgta atcgcccacg
    41341 ggataaacgc actgatgatc agagccagcg gcaccagaat cactttattg atcagcgatc
    41401 ctttcgccac gccccagacc acgggaagtt cccggttggc ccgcacgcct gaaacttgtt
    41461 gcgcattgag cgataagtca tcccctaata caccggcggt tttcttcgcc gccagtttgc
    41521 ccatcacgga aatatcgtcc agcagtgtgg cgatatcatc gagcaacgtc agtaaactac
    41581 tcccggccaa aagcattccc tctcattttt attgttgaag ggtgtaagta tgaagcaaaa
    41641 acggcagctc tgatacttgc ttcacaggtc aacatttttt taacaatcaa gtgcaattat
    41701 atctctcgcc agcagaatga ttttcacgtt tactatgacc caccttttta tttcagctgt
    41761 gagggattat gcgtttccgc cagttgttac cgctttttgg cgcgctgttt gcgttgtata
    41821 tcatttgggg ctcaacctat tttgtcattc ggattggcgt ggaaagctgg ccgccgttaa
    41881 tgatggcggg cgtgcgattc ctggcagccg gtattttatt gctggcattt ttgctactgc
    41941 gcggacacaa acttcccccg ctacgtccgc tgctcaatgc cgcgctgatt ggcctgttat
    42001 tgctggctgt cggtaatggc atggtgacgg ttgccgaaca tcaaaatgtt ccttccggca
    42061 tcgccgccgt agtggttgca accgtgcccc tctttaccct gtgcttcagc cgcctgtttg
    42121 gcattaaaac gcgcaaactg gaatgggtgg gtatcgccat tgggcttgcc ggaatcatca
    42181 tgctcaatag cggtggaaat ttaagcggca atccgtgggg cgcgattctg attttaatcg
    42241 gctcgattag ctgggcgttt ggctcggttt atggctcgcg cattacctta cctgtaggga
    42301 tgatggcggg tgcgattgag atgcttgcgg caggcgtggt gttaatgatt gcgtcgatga
    42361 ttgcgggtga aaaactgacg gcgctccctt ccctttcagg cttccttgcg gtcggctatc
    42421 tggcgctgtt tggttcgatt atcgccatta acgcttatat gtatttaatc cgcaacgtca
    42481 gtccggctct cgccaccagc tacgcctacg ttaacccggt ggtcgcggtc ttactgggta
    42541 cgggactggg tggcgaaacg ctgtcgaaga ttgaatggct ggcgctcggc gtaattgttt
    42601 tcgcggtggt actggtcacg ttgggaaaat atctcttccc ggccaagccc gttgtcgcgc
    42661 cagttattca ggatgcatca agcgagtaaa tgaatcccct gcgtgtcgat ctgcgcgctg
    42721 gcaccttcgc cgcagatcca ctcttccaga cgctcggtaa gcgccgcatc cgtaagcttc
    42781 tcgcgcccac gtaacgcaca ctcccagaca atcagtacgc gccagccgag ttcctgcaag
    42841 cgactgatat cgcggcgatc gcgttcaaca tttttaccta tcttctccag ccagaactcg
    42901 gttcgagtcg caggcacttt aaacagatag cagtgatgat gatgccagaa gcagccgtgg
    42961 gtaaatatca cgcagcgata ttcatcaacg acaaaatccg ggcgtccggg cagactggcg
    43021 tcctgaacgc gaaacgcaag gccctgcccg gttaacaaac tggcgaggcg cttctcaatc
    43081 gccgtatcac gcgtggcaat cgcgcgcata tttttgctgc gggtggcctt atcgtgaacg
    43141 tcgaccatgt tgtgcctctt gctgacgcaa cgccaccgcc tgtttgattt ttggctcaag
    43201 cagttttgcc accgcggcaa agaccggcac gaccaccgag ttaccgaact ggcgataggc
    43261 ctgagtgtcc gaaaccggaa tgcggaattt cacttctcct ggcgcttcaa aacccattaa
    43321 gcgcgcacat tcccgaggcg ttaaccgacg tgggcgatgt tgctgattca gcggatcgtc
    43381 aaagtctttt tcacccgtgg ccatatccca gccgcgatcg attaaaattt ccgcgccatc
    43441 tttgtaataa cgagcagaca gcgtacgcgt gacgctttgc ggattgttcg gataaaccat
    43501 tccataaccg aagccattac cgcgcgcctg atgttttttc gcataacgat agaggtactt
    43561 ccacagcacc ggcgtcagga tatatttcgc ctcgaccatc gggtccagca gctgggccag
    43621 cgtcactcgc tgcgcaggga aacattcgct gatatcacgc agggtaaaat cggctttcag
    43681 attcagatcg cgacgaaaac ccaccagcac gatgcgttca cggtgctgcg gcaggaaatg
    43741 tttgccatcg atgattttcg gatcgtccgg cccgttatct tctgcatcag ccacgtcata
    43801 gcccagttcg tccagcgtct gcatgatgat gcggaacgtt ttaccctggt cgtgactttt
    43861 caggtttttg acgttttcga gcacaaacat cgccggacga cgcgcgtcga taatgcgcac
    43921 cacatcaaag aacagcgtgc cctgagtatc gcaggcaaaa ccgtgcgccc gcccgagcga
    43981 gttctttttc gatacgccag ccagcgaaaa tggctgacaa ggaaaacccg ccagtaatac
    44041 atcgtgttca ggaatgtgtt gacgaatatg ttccgccgcc gcctcatcac tcacgccttc
    44101 tttatggctg agggtgatgt cgcggatatc ttcattaaaa tgatgcgtcg ccgggtcgca
    44161 ataatgattg gctttataag tgcgtaccgc atgtttgttc cattcgctgg taaacacgca
    44221 ctgtccgcca atcgattcaa aaccgcgacg gatgccgcca attccggcaa atagatcgat
    44281 aaagcgaaac gcataatgcg gatgatgtgc cggtggtttg ggtaacaacg tttgcagatg
    44341 ggcgaactct ttctcactta aacggtgcca tgccgagtca ttcgccagcg cacgttttaa
    44401 aattgccgcg ctccagtgat tctcacccac accattaagc tgcgccacca gcgttttaac
    44461 atcataaatt tgcagcagtt tctccagcat tgcctgaacg acattcccgg tgctgtatga
    44521 atcggttact gatatatttt cctgcataga tttaaccggc catctgagat ggccgacagg
    44581 ttaacataat tacagccatt tagggccacg cgctcagccc gcgggtgaaa acgcatctat
    44641 caccttgtga tcgacacctt cattctcccc cgccagttcg gcactgagct tcgccataaa
    44701 ctcgactaaa aattgcgcgt tgtgctgcgc cagctgcttg cctcttgcgg tttgcatggt
    44761 ttgcggtagc ttcagcaact tagtctggaa atgatccagc gcgtagcgtt tatcatccag
    44821 cgggcgatgc tgtgcgaacg gatcttcacc atcaaacagc gccacgccca atgcccctga
    44881 aacagcaaac acgcgcgcca ggccaatcgc ccccagggct tccagccgat ccgcatcctg
    44941 gacgatttta gcttccgttg ttaagggggc gatttgcgcg ctgaaactgt gagcggcaat
    45001 ggcatgacaa acggcctcga ttttctccgc cggaaattgc acgaactctt cacggagcag
    45061 gcgacgcgcc tcttctgccg ccaggattga agaatgctgc cgttgcggat gatttttcgc
    45121 caggctcaca atatcgtgaa aataacaggc ggttaaaatc accagcatat cgacatcgtc
    45181 atctgccgcc agcttctgtg ccgtagccca gacgcggcga aaatgacata cgtcatgggc
    45241 cgcgtcctgg tgctgatgat ggtttttcaa ccagttttcg aactgtgcct gccagtgttg
    45301 taagtccata cgcgctccgt tgccaaagag tcgcaacctt agcagttttt attcatagcg
    45361 tctgctacgt gcgggacgag gtcgggtgta ccaggcaatg ctgcctaaca atgccccgac
    45421 cagagccata aataaaaatc cgaccagtgc ccctaaccac tttcccgcgg tagagcctaa
    45481 atcgctttca aatcccgaca ccggggcatc agggatccgg ctcatcaccc agatgtaacg
    45541 cgccattgcc cagacgaaat agccacacca tgtatagaaa gcccacaaag ccaatttgcc
    45601 gccagggctg cgagagagtc tctcttccat ctttgtcgaa ccgtattcca ttttgagata
    45661 acccggaaaa ttcagactta gtactattat gtgataaaag tcacattttt ccacattgga
    45721 tgagccagtc aagactcaat cattttttga acaaatagat aatttttgcg caacggtagt
    45781 gaatacagga aaaatcgttt tgagcgattt tatttatctt tgatatcggc tttattaatt
    45841 cattcaatca atatattagc actgattaca attatacctt tttattattc tgtgtcatga
    45901 tgcctccgtt attagccttt tatcgtcttg tttatatttt ttgggccggc atgatgccgg
    45961 ctttttttta ttccttcatt aatgtgcgcg tgatcacacc agccgtttgg cgcaacaatc
    46021 attgataccc cctatgtttc cgctcaactc ataacatatt gatatgcatt cataatattc
    46081 gtgaatattt atattcagaa ttcataatta tgaattatat taaatagatt agaaacattt
    46141 tgaaatcttt taaatacatt tgttacatgt aatctttaaa ataaaatgaa cttcatagaa
    46201 tagtatccaa atgtgctttt ttttggataa cggcacttat tgatatattc atgaagatta
    46261 taatcacaag ggaatacata atgaaaagaa aagttctggc aatgctggtc ccggcgttat
    46321 tagttgctgg cgcagcaaat gcggctgaaa tttataataa agatggcaat aaagttgatt
    46381 tctacggcaa gatggttggc gaacgcatct ggtcaaatac tgatgataat aacagcgaaa
    46441 acgaagatac ctcctatgct cgtttcggtg ttaaaggtga aacccaaatc accagcgaac
    46501 tgactggttt tggtcagttt gaatacaacc tcgacgccag caagcccgaa ggctctaatc
    46561 aggaaaaaac ccgtttaacc ttcgcaggtt tgaaatataa cgagttagga tctttcgact
    46621 atggtcgtaa ctacggtgtt gcttatgatg ccgcagctta taccgatatg ttagttgaat
    46681 ggggtggtga ttcctgggcg tccgctgaca acttcatgaa cggtcgtacc aacggtgttg
    46741 caacctaccg taactctgat ttctttggtc tggttgatgg tctgaatttt gctgtgcaat
    46801 atcaaggtaa gaacagcaat cgtgacgtta cgaaacaaaa cggtgatggc tatgcgttgt
    46861 ctgtagacta caacatcgaa ggttttggtt ttgtaggtgc atatagcaaa tctgatcgta
    46921 ctaatgaaca agctggtgac ggctacggtg ataacgctga agtgtggtca ttagcagcca
    46981 agtatgatgc aaataatatc tatgcagcaa tgatgtacgg tgaaacccgc aacatgaccg
    47041 ttttggctaa tgatcatttt gcaaataaaa cccaaaactt tgaagctgtt gtacagtatc
    47101 agttcgactt cggtttacgt ccgtctttag gctacgtata ttccaaaggc aaagatcttt
    47161 atgctcgtga tggacataaa ggtgttgatg ctgaccgcgt aaattatatc gaagttggta
    47221 cctggtacta cttcaataag aacatgaacg tctacacagc atacaaattt aacctgctgg
    47281 ataaagacga tgcagcgatt accgatgccg caactgatga ccagtttgca gttggtatcg
    47341 tctaccagtt ctaatcagcc aacgcgccgc ggcgtgttac tgtaaaactt aacaccagcc
    47401 tgagttcgtc cctcatcgaa ctcaggcttt tttattggca tttcatctcc atctgcggca
    47461 cacgcaattg ccaaagcata tcgatacagc accttacccc acccctcctg gctcaataaa
    47521 atctaccagc gtctcttccg gccaacatcc aaatgcattt ctttatgaat taatccgcaa
    47581 caaaaccgcc gtccatatag aaatatttgc aaaaatagtg catgaaaaaa gctttttctc
    47641 taccaatccg cgatataaat attcatcagc accacaatat tccacagcac taaatctctc
    47701 cctgccgccc cggacctctg ataatggtct aaaatcattg aggccacttg cgacgacgta
    47761 gctcaataag gaaaatttgg cgtgaataat attcaacacc agtgcagcat catcattaca
    47821 tacttatgcc cgccaactac cgctttcgat atattgactc aatttttcag cctggttaaa
    47881 aatagcgacc agctcagtcg caaatatagt gactacccta actaagcaac aataaggaat
    47941 acactatgac tgttcaaaca agtaaaaacc cgcaggtcga tattgctgaa gataatgcat
    48001 tcttcccttc agaatattcg cttagccaat ataccagtcc cgtctctgat cttgatggcg
    48061 tggactatcc aaaaccgtat cgcggtaaac ataaaattct ggtgatcgcc gcggacgaac
    48121 gttatttgcc gaccgataac gggaaactat tctcgaccgg taaccatccg attgaaacgt
    48181 tgctgccgtt gtatcatctc catgctgcag gtttcgaatt cgaagtggcg accatttccg
    48241 gtctgatgac caagtttgaa tactgggcta tgccacacaa agatgaaaaa gtaatgccat
    48301 tctttgagca gcataaatcg ttgttccgca atccgaagaa actcgcggat gttgtcgcaa
    48361 gcctcaacgc tgatagcgaa tatgcagcaa tctttgttcc tggtggtcat ggcgcactta
    48421 ttggtttacc tgaaagccag gacgtagctg ccgctttaca gtgggcaatc aaaaatgacc
    48481 gttttgttat ctccctttgc cacggcccgg cggcttttct ggcgcttcgc cacggcgata
    48541 acccactgaa tggttattcc atttgcgcat tcccagacgc cgcagacaaa caaacgccag
    48601 agataggcta tatgccgggt catctcacct ggtacttcgg cgaagagctg aagaaaatgg
    48661 gcatgaacat cattaatgac gacatcaccg ggcgagtaca taaggaccgt aaagttctca
    48721 ccggcgacag tcctttcgca gcgaatgcgt tgggtaaact ggcggcgcag gaaatgctgg
    48781 cagcttacgc gggttaatcc ctgttacatt gtaatgtaag cgcattatca atcgcagcca
    48841 cgttaatact cacgacgtga ctgcgttttt tcacggttaa ttttggcgta ctgaagccct
    48901 atattaattt ctttgcggta acgtaattcg gaacacgtta tgcttattga gatagtgata
    48961 cgtagcactt cccccatgca attcggcaat cgctttgact aaagaaagac caagtccctg
    49021 acctacggaa tgacgcgaat tatctccccg ccaaaatcta cggaagagtt tttcaggctc
    49081 atgaattttc gttccaggac tggcgatatc aatattaaga gagccgttgg tatcaagaaa
    49141 actggttatc tgaatgcgcg atttttctgg tgaatatcga atagcgttaa caataagatt
    49201 cgataacatt cgttgcagta aaattttatc cgcaaagatt tgctgattgc actcgacctt
    49261 aaggcaaatc tctttctcgt ctgaaaggta ttcaagatag tccaacaaat tttcgacttc
    49321 cttattgaga gaaagcgaat ccagtttcac caaaacattg tttttatctg ccctggcaag
    49381 aaacagtatg ttctctgtta accgcgaaat attctccagc tcttcaatgt ttccggcaat
    49441 tgttttttga tattcagcga tacttctggt ttgactgagc gtaacctgat tctgacccag
    49501 taatgcatta attggtgttc taagttcatg agcgagatcg tcagcaaact gacttagacg
    49561 ctcaaaatct ttgactaaag catgatgcat tttattcaac gcctgcccaa gaggttttag
    49621 ttctctcggt aatgcgctaa cctcaacagg ctcccggtta tcgttataat tcagcgcttc
    49681 cgttacacca ctcaactttt tgatctctcg taaccccgtt ctgattaaca gcggacttaa
    49741 tactgagcaa agtacaatgg cgacaatgca aattataatg ctattaattt tatactgttc
    49801 aagcatgtta tgtctggctg aagccaattt agccacagta accgttaatg atggcgaaac
    49861 ttcatcaata ttaattcgta aagcatctat ctctgtgtca ttaataatgc ttctgtaaat
    49921 gccagctgcg ctgattgtct cactagcagg tatgttattt aacatgccat cactgacatt
    49981 tgtccggtta acaattttat tgatgccatg accatgaatg atcaagatat cctgactaac
    50041 atccatcatc cggttaaaat agacaggtaa cgtatctgga tttaccccat caattaacaa
    50101 ctgcttgatc tgcgctgtcc ggttaatgag tgttgtatca tcgcgccatt tcaactcact
    50161 tgccaggcca ttatagagag tccatacaat tccagcgcca gcaacagaca gcaccaatat
    50221 aaaaagcaag tttaaacgga cggttataga taattttttc attatttttt taccgctacg
    50281 aatgaatatc ccatcccacg gattgtggca atgagctttt caggaaaagg atcatcaact
    50341 tttgcgcgga gcctgcgaat ggcgacatcc accgtattgg tatcactatc aaagttgatt
    50401 ccccaaattt cactcgcaat aaccgttctg ggtataattt cgccagctct ggaggccagt
    50461 agccaaagta actgaaactc cttgcgcgtc agtgtaatac tgatattgtt cctgctcaca
    50521 ctttgactaa cagagtccat tcttaagcca ctgatttcta atgttgaatt caaagcgtga
    50581 tgttgcctta attgtgcccg aacccttgcc agcaactcag aaaatgaaaa aggttttacc
    50641 agataatcat ttgctccact gtccagccct ctgactctgt catcgacaga atcccttgct
    50701 gtaaggcaaa taacaggagt ttgctttgcc gttcttaacg tttgtaagat ctgccagcca
    50761 tccatacccg gaagcataat atccagaatg atcaatgcat aatcatcctt cagcgcaaga
    50821 taaagcccat ctctgccatc agaaacggca tcaatgacat aacccgcttc ggaaagcccc
    50881 tgcgttaccc actcctgggt cctttgatta tcttcaataa gtaaaatctt catgaaaata
    50941 atatgccata aatcatatgt tatagaaaca gcctggttca ttacaaaatt gtaatgctgc
    51001 tgtaaggtta ccctggacgc tttttcgcta tcctcaaaac tcattcacat gacaaggata
    51061 taaacatgtt aaagcgttat ttagtactct ccgtagtaac ggctgcattt tcattacctt
    51121 ctttggtcta tgctgcacaa caaaacattc ttagcgtgca cattttgaac cagcaaacag
    51181 gaaaacccgc tgccgacgtg acagtcactc ttgaaaagaa agcggataac ggctggttac
    51241 aacttaatac cgccaaaaca gataaggatg gacgaattaa ggcactgtgg cccgagcaaa
    51301 ctgcgactac gggcgattac cgtgtcgtat ttaaaaccgg ggactatttc aagaaacaaa
    51361 atcttgaaag tttcttccct gagatccccg ttgaatttca tattaataaa gtgaacgagc
    51421 attatcatgt gcctttatta cttagccaat atgggtattc aacctatcgt ggcagttaat
    51481 gcataaatat ataaaagcag atctctgcta ctgttaggga ggtgactccc tgacagatta
    51541 acagtaaacg gctcttgctg gctaacgaca aaaaagtgtg atggcttatg aaaaagaatc
    51601 aatttttaaa agaatcagat gttacggccg agtcggtatt ctttatgaag cgtcggcagg
    51661 tgttaaaagc actgggcatc agcgcggctg cactatcttt gcctcacgct gcgcatgccg
    51721 atctgcttag ctggtttaaa gggaacgatc gtccacccgc ccccgccgga aaaccgctgg
    51781 agttcagcaa gcctgccgcc tggcaaaata acctgccact gacgccagca gataaagttt
    51841 ccggttataa caacttctat gaattcgggc tggataaagc cgatcccgcc gctaatgctg
    51901 gtagcctgaa aaccgatcca tggacactga aaatcagcgg cgaagtagca aaaccattaa
    51961 ccctcgatca cgatgattta acccgtcgct tcccgctgga agagcgtatt tatcgtatgc
    52021 gctgcgtgga agcatggtcg atggtggtgc cgtggattgg ttttccgctg cacaaattgc
    52081 tggcgcttgc cgaacccacc agcaatgcga agtatgtcgc tttcgaaaca atttatgcac
    52141 cggaacagat gccaggccag caggaccgct ttatcggcgg cgggctgaaa tatccttatg
    52201 tcgaaggatt gcgtctcgac gaagcaatgc atccgctcac actgatgacc gtaggtgttt
    52261 atggcaaggc gttaccgcca caaaatggcg cgccggtgcg actgattgtg ccgtggaaat
    52321 atggctttaa agggattaaa tcgatcgtca gtattaagct gacccacgag cgtccgccaa
    52381 ccacctggaa tctggcagcg cctgacgaat acggttttta cgccaacgtt aatccgcatg
    52441 ttgatcaccc gcgctggtca caggctaccg aacgatttat tggttcaggc ggcatcctcg
    52501 atgtacagcg ccagccaacg ctactgttta atggttacgc cgagcaggtg gcatcgctgt
    52561 atcgtggcct ggatttgcgg gagaatttct aaatgcgtct gacagcaaaa caggtgacat
    52621 ggctgaaagt ttgcctgcat cttgccggat tgttgccgtt tctctggctg gtctgggcga
    52681 ttaatcacgg tggattgggt gccgatccgg tgaaagatat tcagcatttt accggtcgca
    52741 ctgcactgaa atttttgctt gcggccttgt taatcacacc tctggcacgc tacgcaaaac
    52801 agccgttatt gatacgcact cgccgcctgt taggcttatg gtgctttgcc tgggcgacac
    52861 tgcatttaac cagttacgca ttactggagc tgggcgtgaa taatctggcg ttattaggta
    52921 aggagttaat tacccggcct tatttaacgt taggaattat cagctgggtt attttgcttg
    52981 ctttagcgtt tacttcaacc caggcgatgc agcgaaaact cggcaagcac tggcaacagt
    53041 tgcataattt cgtctatctt gtcgcgatcc tggccccaat acattatctg tggtcagtga
    53101 agattatttc accgcagcca ctcatctacg ctgggctggc ggtactgctt ttagccttac
    53161 ggtataagaa gttgctttcc cttttcaacc ggttacgcaa gcaggttcac aacaaattat
    53221 ctctataact tgatattgcc gacaaagtac gcgttatccc tgccggatgc ggcgcgagcg
    53281 ccttatccgg catacacaga tcgcattagg ttatgtttcg tttgtcatca acctcaaatc
    53341 atcgacataa tttattttat ttcaataaga tagataagca cacctgagag aactattctc
    53401 ttgtctccta agagttgttg cattttgtta tatgttacaa tataacatta cacatcatat
    53461 acattaactc tggaggaaac tgttttggcg attcgtcttc ataaactggc tgttgcttta
    53521 ggtgtcttta ttgttagcgc tcctgccttt tcgcatggtc atcactcaca cggcaaaccc
    53581 ttaacagagg tcgaacaaaa agctgctaat ggtgtttttg atgatgccaa tgtacaaaac
    53641 cgaacgctaa gtgactggga tggagtctgg caatccgttt atcctttatt gcaaagtggc
    53701 aaacttgacc ccgtctttca gaagaaagcg gatgcagata aaactaaaac atttgctgaa
    53761 attaaagatt attatcacaa aggttatgca acagatatcg agatgattgg cattgaggac
    53821 ggcattgttg aattccatag aaataatgaa acaacatcct gtaaatatga ttacgatgga
    53881 tacaaaatac tcacctataa atcaggcaag aaaggcgttc gttacttatt cgaatgtaaa
    53941 gatcctgaaa gcaaagcccc taaatatata caatttagcg atcatataat tgcaccacga
    54001 aaatcatctc attttcacat ttttatgggt aatgattccc agcaatcttt attgaatgaa
    54061 atggaaaact ggccaacgta ttatccctat cagttgagta gcgaagaagt ggtcgaggag
    54121 atgatgtctc attgattttt ttgcgctgac ttcagttatt taacagaaca gtgcctggta
    54181 gtaacaacaa taatttacca agacaaataa gtaagacaaa ctcagacacg ctcatacttg
    54241 agtcgtgtct tttttattat gtctgtagaa aaaaactgcg aaaaaggcga acaataaata
    54301 cacaactcag ccatacagac tggcagcgca aatgtttcca ggagcaagtc atctgtcatg
    54361 gtaaagctaa ccagaaatat aggttaattt tggtgtttaa acctttttta cttgttagta
    54421 tgatacaaag gctttcaaaa aagctgggaa attcaacaac tcttggaaat tatggacttt
    54481 gtcccgcggc tttaatctga cggccgcgtt ccttttttgg ctcaattatt gaccccttct
    54541 ctacgcaact tcagttcccg ccagcaactt tgcggcagtt tcataggatc aatgtctaaa
    54601 agaataatgg tgatcgataa gaaaacgact gaataactgt agattttcgc tcgaaacctt
    54661 cctgtcagct ccatagcgaa tcaagtgctg aatgtcacag tatcgaacag aaaacagtga
    54721 cgatctaacc cttcaagaat attctacgat tgttctgttt aggaaaagca aggcgggaag
    54781 tcgggagata agtcattgat aaagtggcgg agagaggggg atttgaaccc ccggtagagt
    54841 tgcccctact ccggttttcg agaccggtcc gttcagccgc tccggcatct ctccgttcag
    54901 atggttgcca tgatgccagg aaatttggca ttttaacagt ccctgtccgt gcaattttgt
    54961 tcaagtgacg agtttgcgag caaaacgatg attaagtggc cctggaaagt acaagaatca
    55021 gcacatcaaa ctgcccttcc ctggcaggaa gcactatcga tccccctttt aacgtgtctg
    55081 acggaacagg aacaaagcaa attagtcact cttgccgaac gttttttaca gcaaaagcgg
    55141 cttgttcctt tacagggctt tgaactggat tcattaagaa gctgccggat agcacttcta
    55201 ttttgcctgc ccgttctgga gttaggactg gaatggctgg atggttttca tgaagtctta
    55261 atttatcctg cgccatttgt ggtcgatgat gaatgggaag acgatatcgg tctggtgcat
    55321 aaccaacgta ttgttcagtc aggtcagagc tggcagcaag ggcctatcgt tttgaactgg
    55381 ttggatatac aagattcttt tgatgcttct ggttttaacc tgattattca tgaagtcgct
    55441 cataagctgg acacccgtaa cggcgatcgc gccagcggag ttccctttat ttcgttgcgt
    55501 gaggttgctg gctgggaaca cgatcttcat gctgcaatga acaacattca ggaagaaatc
    55561 gaattagttg gtgagaatgc ggcgagcatt gatgcttatg ctgccagtga tcctgctgaa
    55621 tgttttgccg tactttctga atatttcttt agcgccccag aactttttgc tcctcgtttc
    55681 ccttcattgt ggcaacgttt ctgtcaattt tatcaacaag atcctttgca gagactgcat
    55741 cacgctaatg atacagactc gttttcggcg acgaatgttc attaattaac aactttgcag
    55801 attaattaac caattgaaat ggcttatgaa atttagtgtt gacagacaag gtaccgctaa
    55861 gtaatatgcg ccccgttctc acgattcctc tgtagttcag tcggtagaac ggcggactgt
    55921 taatccgtat gtcactggtt cgagtccagt cagaggagcc aaattctaaa aattcgcttt
    55981 ttagcgcaat gtcactgacc ttagttgagc attgcttttt aacggatagc gggtttttga
    56041 catcttaagc gccctcgacc tttatggttg agggcgtttt gctatgaacg ccatcaccat
    56101 tttcccctcg attacaaaac ttgagtaatt cagtagtctc ccctcttgca actcacaccc
    56161 aaaactgctt aacgaaaagt tattaatttt caatcatatt gctatcagga tttacatttt
    56221 ttcgctgtgc tagaaagggc gcatttatgt tagctcgttc agggaaggta agcatggcta
    56281 cgaagaagag aagtggagaa gaaataaatg accaacaaat cttatgcggg atgggtatta
    56341 aactacgccg cttaactgcg ggtatctgcc tggtaactca acttgtgttc cctatggctg
    56401 cggcagcaca aggtgtggta aacgccgcaa cccaacaacc agttcctgca caaatcgcca
    56461 ttgcaaatgc caatacggtg ccctataccc ttggagcgct ggaatcggcc caaagcgttg
    56521 ccgaacgttt cggtatttcg ttggctgagt tacgcaaact caaccagttt cgtacgtttg
    56581 ctcgaggttt tgataatgtc cgccagggtg atgaactgga tgtcccggca caagttagtg
    56641 aaaaaaaatt aaccccgccg ccgggtaata gcagtgacaa ccttgagcaa cagatagcca
    56701 gtacttcaca gcaaatcggg tctctgctcg ccgaagatat gaacagcgag caagcggcca
    56761 atatggcgcg tggatgggcc tcttctcagg catctggcgc attgacagac tggttaagcc
    56821 gcttcggtac cgcaagaatc acgctgggcg tggatgaaga ttttagcctg aagaattccc
    56881 agttcgattt tctccatcca tggtatgaaa cgcctgataa tctctttttc agccagcata
    56941 ccctccaccg tactgacgaa cgtacgcaga ttaacaacgg cttgggttgg cgtcatttca
    57001 ctcccacatg gatgtcgggc atcaacttct ttttcgacca cgatcttaag cgcagaaacg
    57061 agctatggtg gggcgcaaac agtagatata acgctggtgg caggcccggc agacgcctcg
    57121 cagtccgtcc ttaaaaacaa ccggtcatca ctgaaagggg actttaccga aagtgctgaa
    57181 ctatatcttg ttttacacga tctatcaggt catccgatca atgtttctga agggctggaa
    57241 tttgtgcaat caggtaccaa cgtgtcctat gtgcaaatca gcacaattga ttacactcaa
    57301 aatttatacg gtgagtataa agccactgtt acaggcggcg gagagggtat cgcaacgctg
    57361 atccccgtgt tgaatggtgt tcaccaggcg ggtctgagta ccacgataga attcattagc
    57421 gcgggaacca gaccaatgaa cggtacagta tcagtgaatg gtgcaaacct acctgcagct
    57481 tcattccctt cgcagggatt taccggggcg tattatcagt tgaataatga caactttgca
    57541 ccaggaaaaa cggcggctga ttatgcgttt tcaagcactg cctcctgggt tgatgttgat
    57601 acttccggta aggtgacatt caaaaatgtt ggagatagaa atgctgtaat aatcacggcg
    57661 accccaagat caggtggcgc tatttaccaa acgcaagtac gggtgaaggg ctggtgggtg
    57721 aatcacggta ataacctcat gcaattaagc caggctgaaa actactgcag caaccaggtc
    57781 ggtaatggct acccccttcc ccgcgcggac cttttaagca atgggcacat gcggcgcgaa
    57841 atagggtcac tgtatggtga gtggggagat atgggaaatt acatgaagga agctgatttt
    57901 tattcaatgg tatattggtc ttctaattcc gcaggcgcag gccaacaata cattgtatcc
    57961 ctggaaacag ggacgcaaaa cacttatcaa acgtatgaat ttttttatgg ggcgtgttat
    58021 aaacagattt aatttttcgt tataatttct tacagagtgc tccattcggg gcgctctttt
    58081 ttacattctc tctacagaac ttgcccttct ctcacagaaa atgaataaat aaaaatgcga
    58141 taccgccaga ttcgcgttcg gtgtgactat ggttgtatcc ggtgagtaca gctttgatcc
    58201 aaaggctggc tttctgtaat gattgaccag ccacaaagta ttgatgacac caccgttcat
    58261 aacagatcgg cactactatg cataagtact gcctcatatt ctcaacaaaa cactttttaa
    58321 tgcctttgcg aattcatcca gacttatatt gtcaattttt tgcccaacga cgactgactc
    58381 cgagacatcg gccattgcgg gccatcgcgt tttgtaatca gcataaattg caacaacggg
    58441 ggtattatag gcacttgcca tatgtaatat tgaggtgtca ggagttattg cgatataagc
    58501 atcttttatg attgctgccg aacgtttaat cgagggtgat aaggataaac ggaagacatt
    58561 attataacaa tccactaaca ccctggcttt ttcttcgcct tttggtccat gaacgatcac
    58621 tattggtata tctgtttcac tttgaatttt ttctattaga ttttctgcaa tcgataatga
    58681 aaatgtacgt tcctgcgagc taccttctaa attaagcgca atgtaagatc ctaacgatcg
    58741 catttcctcg cgcacctcat cgagtacatc cttacttagt ggcaattcat attttggcct
    58801 gactgctgga aaacccgcct cacgcatcat aaatgcccat gtcatgggta caggagcccg
    58861 gagattctga tccatccggg acgcgctctt acacaaaggg gaatagcact tcatggttaa
    58921 accaacaacc tgaaaattcg tttttgcttt caactgactg ataaatatca tcgttttcag
    58981 gttcttttta cgcatcgcct ctatacaaag atccggcgta ccgtattgct gtgttatgtt
    59041 cttcgctaga tcttttattt cttttaatgt tgcgtgatcc tgcatagtca ttgtaactaa
    59101 tgttaattta gtctgttcaa gtttgagcgc attaaagact tctaaattaa ttgtcgacgt
    59161 tacaattaaa agatgcttaa ttttatgcaa ttcaagcgcc cgaataacag gaaagatggc
    59221 catagcatcg ccaatctgat cgggaatatg gatgacaaca aagtctgttt tttcaatatt
    59281 gaaattataa gctttataat cgtagtaact aaatgcaata cgtctcaaca atgatgctaa
    59341 aaacatacct aacctcgcct ccctactggt tataatgcaa tgcagtctat cagactcatc
    59401 agggtgccat tttgtgcata tgcggacttt tatgtttcat atctcgaacc tgtgaggcct
    59461 ctgcttaatc cttaaacaac accagcaact cctgcgcttt catcttccag cgaatttttc
    59521 atgttgctgc tcatcagcca taaaatcatt tgcagatgcg ctctgtcgag gtagtctcat
    59581 aaggttcgtt tatagatcga cggcaatgtg agttaccttt tccatactaa ttataaaaag
    59641 acagtacaaa caggatcatt atggactcca cgctcatctc cactcgtccc gatgaaggga
    59701 cgctttcgtt aagtcgcgcc cgacgagctg cgttaggcag tttcgctggt gccgtcgtcg
    59761 actggtatga ttttttactc tatggcatca ccgccgcact ggtgtttaat cgcgagtttt
    59821 tcccgcaagt aagcccggct atgggaacgc tcgccgcatt tgccaccttt ggcgtcggat
    59881 tcctgttccg tccgctcggc ggtgtcattt tcggtcactt tggcgaccga ctgggacgta
    59941 agcgcatgtt aatgctgacc gtctggatga tgggcatcgc gacagccttg attggtattc
    60001 ttccttcatt ctcgaccatt gggtggtggg cacctatttt gctggtgaca ctgcgtgcca
    60061 ttcagggatt tgccgtcggc ggcgaatggg gaggcgcggc gttgctttcc gttgaaagtg
    60121 cgccgaaaaa taaaaaagcc ttttacagta gcggtgtaca agttggctac ggtgtaggtt
    60181 tactgctttc aaccggactg gtttcattga tcagtatgat gacaactgac gaacagtttt
    60241 taagctgggg ctggcgcatt cctttcctgt ttagcatcgt actggtactg ggagcattgt
    60301 gggtgcgcaa tggcatggag gagtccgcgg aatttgaaca acagcaacat aatcaagcgg
    60361 ccgcgaaaaa acgcatcccg gttatcgaag ctctgttacg acatcccggt gctttcctga
    60421 agattattgc actacgactg tgcgagttgc tgacgatgta cattgttact gcctttgcac
    60481 ttaattattc aacccagaat atggggttac cgcgcgaact tttccttaat attggtttgc
    60541 tggtaggtgg attaagctgc ctgacaattc cctgttttgc ctggcttgcc gatcgttttg
    60601 ggcggcgcag ggtttatatc acaggtgcgt taatcggaac gttgagcgca tttcctttct
    60661 ttatggcgct tgaagcacaa tctattttct ggatagtttt cttctccatc atgctggcaa
    60721 acattgcgca tgatatggtg gtgtgtgtac aacaaccgat gtttaccgaa atgtttggtg
    60781 ccagttatcg ctatagtggc gctggagtcg gttatcaggt tgccagtgtg gttggcggtg
    60841 gatttacacc ttttattgcc gctgcactca tcacttactt tgccgggaac tggcatagcg
    60901 tcgccattta tttgctggct ggatgcctga tttccgcaat gaccgctttg ttgatgaaag
    60961 acaatcaacg cgcttaatag cctggcgaag atcatccgat cttcgcctta cacttttgtt
    61021 tcacattcct gtgacatact atcggatgtg cggtaattgt atggaacagg agacacacat
    61081 gaataataag ggctccggtc tgaccccagc tcaggcactg gataaactcg acgcgctgta
    61141 tgagcaatct gtagtcgctt tacgcaacgc cattggcaaa tatattacaa gtggcgaatt
    61201 acctgatgaa aacgcccgca aacaaggtct ttttgtctat ccatcactga ccgtaacctg
    61261 ggacggtagc acaaccaatc cccccaaaac gcgcgcattt ggtcgtttta cccacgcagg
    61321 cagctacacc accacgatta ctcgccctac tctctttcgt tcgtatctca atgaacaact
    61381 tacgttgctg tatcaggatt atggtgcgca tatctcggtg caaccctcgc agcatgaaat
    61441 cccttatcct tatgtcatcg atggctctga attgacactt gatcgctcaa tgagcgctgg
    61501 gttaactcgc tacttcccga caacagaact ggcgcaaatt ggcgatgaaa ctgcagacgg
    61561 catttatcat ccaacggaat tctccccgct atcgcatttt gatgcgcgcc gcgtcgattt
    61621 ttccctcgcg cggttgcgcc attataccgg tacgccagtt gaacattttc agccgttcgt
    61681 cttgtttacc aactacacac gttatgtgga tgaattcgtt cgttggggat gcagccagat
    61741 cctcgaccct gatagtccct acattgccct ttcttgtgct ggcgggaact ggatcaccgc
    61801 cgaaaccgaa gcgccagaag aagccatttc cgaccttgca tggaaaaaac accagatgcc
    61861 agcatggcat ttaattaccg ccgatggtca gggtattact ttggtgaata ttggcgtggg
    61921 accgtcaaat gctaaaacca tctgcgatca tctggcagtg ctacgcccgg atgtctggtt
    61981 gatgattggt cactgtggcg gattacgtga aagtcaggcc attggcgatt atgtacttgc
    62041 acacgcttat ttacgcgatg accacgttct tgatgcagtt ctcccgcccg atattcctat
    62101 tccgagcatt gcggaagtgc aacgtgcgct ttatgacgct accaagttag tcagcggcag
    62161 gcctggtgaa gaagtcaaac agcggttacg tactggcacg gtggtaacca cagatgacag
    62221 gaactgggag ttacgttact cagcttctgc acttcgtttt aacttaagcc gggccgtagc
    62281 aattgatatg gaaagtgcaa ccattgccgc gcaaggatat cgtttccgcg tgccatacgg
    62341 gacactactg tgtgtttcag ataaaccgtt acatggtgag attaaacttc ccggccaggc
    62401 taaccgtttt tatgaaggcg ctatttccga acacctgcaa attggcattc gggcgatcga
    62461 tttgctgcgc gcagaaggcg accgactgca ttcacgtaaa ttacgaacct ttaatgagcc
    62521 gccgttccga taataagtta tgaaccttct tatcagaaat agatgtgaag gattaagtaa
    62581 gacatctggc aaatagcctg caataaacgc tggctgtttg cttccttggg cagatgcgag
    62641 ttttattatc gtcttaatga tttccacata ttaaaa
//
LOCUS       DAZGQL010000017        61734 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.017, whole genome shotgun sequence.
ACCESSION   DAZGQL010000017 DAZGQL010000000
VERSION     DAZGQL010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 61734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61734
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.017"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            173..799
                     /gene="ydhY"
                     /locus_tag="ACHKIR_001264"
     CDS             173..799
                     /gene="ydhY"
                     /locus_tag="ACHKIR_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HFO9989022.1"
                     /translation="MNPVDRPLLNIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTHRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            820..2922
                     /gene="ydhV"
                     /locus_tag="ACHKIR_001265"
     CDS             820..2922
                     /gene="ydhV"
                     /locus_tag="ACHKIR_001265"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HFO9989023.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIRWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAERWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEDMTQEKLDLAAERIFTLHRAYTVKLMQTKDMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRKTLQR
                     LGLEDIAADLAAHNLLPA"
     gene            2935..3573
                     /gene="ydhW"
                     /locus_tag="ACHKIR_001266"
     CDS             2935..3573
                     /gene="ydhW"
                     /locus_tag="ACHKIR_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310406.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HFO9989024.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARAVFLSPPYSVAEEELSVLLENIKQNGDHADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDIAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            3637..4305
                     /gene="ydhX"
                     /locus_tag="ACHKIR_001267"
     CDS             3637..4305
                     /gene="ydhX"
                     /locus_tag="ACHKIR_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFO9989025.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTMQEKKVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQENKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            4302..5087
                     /gene="phsC"
                     /locus_tag="ACHKIR_001268"
     CDS             4302..5087
                     /gene="phsC"
                     /locus_tag="ACHKIR_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HFO9989026.1"
                     /translation="MNPSQHAEQFQSQLANYVPLFTPQFWPVWLIIAGLLLVGMWLVL
                     GLHALLRARGVKKSANDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFALVGAT
                     TVKSLVAVHEVCGFLLLACWLGFVLINAVGGNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDMFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            5091..5903
                     /gene="ydhT"
                     /locus_tag="ACHKIR_001269"
     CDS             5091..5903
                     /gene="ydhT"
                     /locus_tag="ACHKIR_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdhT"
                     /protein_id="HFO9989027.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGERAFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGEDSTIVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRAGVRYRLNEQHQFVEC"
     gene            complement(5909..7519)
                     /gene="ydhS"
                     /locus_tag="ACHKIR_001270"
     CDS             complement(5909..7519)
                     /gene="ydhS"
                     /locus_tag="ACHKIR_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001602606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HFO9989028.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPIYCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFHRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EVGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYEMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEEIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAIAQAVVKPASRARRRLSFNQM"
     gene            complement(7645..7950)
                     /gene="ydhR"
                     /locus_tag="ACHKIR_001271"
     CDS             complement(7645..7950)
                     /gene="ydhR"
                     /locus_tag="ACHKIR_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HFO9989029.1"
                     /translation="MATLLQLHFSFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTNEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            complement(8058..8134)
                     /locus_tag="ACHKIR_001272"
     tRNA            complement(8058..8134)
                     /locus_tag="ACHKIR_001272"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8098..8100),aa:Val,seq:gac)
     gene            complement(8139..8215)
                     /locus_tag="ACHKIR_001273"
     tRNA            complement(8139..8215)
                     /locus_tag="ACHKIR_001273"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8179..8181),aa:Val,seq:gac)
     gene            8524..9780
                     /gene="ydhQ"
                     /locus_tag="ACHKIR_001274"
     CDS             8524..9780
                     /gene="ydhQ"
                     /locus_tag="ACHKIR_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFO9989030.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDKGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPGGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSTYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGADLQQSTITVQQGGVLILDGSTVKGDSVTFSVGSINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVTGDIHVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            complement(9821..11194)
                     /gene="mdtK"
                     /locus_tag="ACHKIR_001275"
     CDS             complement(9821..11194)
                     /gene="mdtK"
                     /locus_tag="ACHKIR_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HFO9989031.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRLPSAI
                     ILQRAAR"
     gene            11409..12050
                     /gene="ribE"
                     /locus_tag="ACHKIR_001276"
     CDS             11409..12050
                     /gene="ribE"
                     /locus_tag="ACHKIR_001276"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HFO9989032.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            complement(12090..13238)
                     /gene="cfa"
                     /locus_tag="ACHKIR_001277"
     CDS             complement(12090..13238)
                     /gene="cfa"
                     /locus_tag="ACHKIR_001277"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HFO9989033.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(13529..14740)
                     /gene="punC"
                     /locus_tag="ACHKIR_001278"
     CDS             complement(13529..14740)
                     /gene="punC"
                     /locus_tag="ACHKIR_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside transporter PunC"
                     /protein_id="HFO9989034.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVDCQNH
                     GNAEVAHSESH"
     gene            14853..15785
                     /gene="punR"
                     /locus_tag="ACHKIR_001279"
     CDS             14853..15785
                     /gene="punR"
                     /locus_tag="ACHKIR_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator PunR"
                     /protein_id="HFO9989035.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            complement(15782..16807)
                     /gene="purR"
                     /locus_tag="ACHKIR_001280"
     CDS             complement(15782..16807)
                     /gene="purR"
                     /locus_tag="ACHKIR_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HFO9989036.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            17106..17195
                     /gene="cydH"
                     /locus_tag="ACHKIR_001281"
                     /gene_synonym="ynhF"
     CDS             17106..17195
                     /gene="cydH"
                     /locus_tag="ACHKIR_001281"
                     /gene_synonym="ynhF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I accessory subunit CydH"
                     /protein_id="HFO9989037.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVVVGLTAALH"
     gene            17361..18530
                     /gene="ydhP"
                     /locus_tag="ACHKIR_001282"
     CDS             17361..18530
                     /gene="ydhP"
                     /locus_tag="ACHKIR_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9989038.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGAGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(18676..19257)
                     /gene="sodB"
                     /locus_tag="ACHKIR_001283"
     CDS             complement(18676..19257)
                     /gene="sodB"
                     /locus_tag="ACHKIR_001283"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HFO9989039.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(19385..20212)
                     /gene="mepH"
                     /locus_tag="ACHKIR_001284"
     CDS             complement(19385..20212)
                     /gene="mepH"
                     /locus_tag="ACHKIR_001284"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HFO9989040.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASANFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            20547..20894
                     /gene="grxD"
                     /locus_tag="ACHKIR_001285"
     CDS             20547..20894
                     /gene="grxD"
                     /locus_tag="ACHKIR_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HFO9989041.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            complement(20945..25561)
                     /gene="lhr"
                     /locus_tag="ACHKIR_001286"
     CDS             complement(20945..25561)
                     /gene="lhr"
                     /locus_tag="ACHKIR_001286"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFO9989042.1"
                     /translation="MADNLNPSSPLPDVFSPATRDWFLRAFKQPTAVQSQTWHVAARG
                     EHALVIAPTGSGKTLAAFLYALDQLFREGGEDTREAHKRKTSRILYISPIKALGTDVQ
                     RNLQIPLQGIADERRRRGETEVNLRVGIRTGDTPAQERSKLTRNPPDILITTPESLYL
                     MLTSRARETLRGVETVIIDEVHAVAGSKRGAHLALSMERLDTLLHTSAQRIGLSATVR
                     SASDVAAFLGGDRPVTVVNPPAMRHPQIRIVVPVANMDDVSSVASGTGEDSHAGREGS
                     IWPYIETGILDEVLRHRSTIVFTNSRGLAEKLTARLNELYAARLQRSPSIAVDAAHFE
                     STSGATSNRVQSSDIFIARSHHGSVSKEQRAITEQALKSGELRCVVATSSLELGIDMG
                     AVDLVIQVATPLSVASGLQRIGRAGHQVGGVSKGLFFPRTRRDLVDSAVIVECMFAGR
                     LENLTPPHNPLDVLAQQTVAAAAMDALQADEWYSRVRRAAPWKDLPRRVFDVTLDMLS
                     GRYPSGDFSAFRPKLVWNRETGILTARPGAQLLAVTSGGTIPDRGMYSVLLPEGEEQA
                     GSRRVGELDEEMVYESRVNDIITLGATSWRIQQITRDQVIVTPAPGRSARLPFWRGEG
                     NGRPAELGEMIGDFLHLLADGAFFSGTIPPWLAEENTIANIQGLIDEQRNATGIVPGS
                     RHLVLERCRDEIGDWRIILHSPYGRRVHEPWALAIAGRIHALWGADASVVASDDGIVA
                     RIPDTDGKLPDAAIFLFEPEKLLQIVREAVGSSALFAARFRECAARTLLMPGRTPGHR
                     TPLWQQRLRASQLLEIAQGYPDFPVILETLRECLQDVYDLPALERLMRRLNGGEIQIS
                     DVTTTTPSPFAASLLFGYVAEFMYQSDAPLAERRASVLSLDSELLRNLLGQVDPGELL
                     DPQVIRQVEEELQRLAPGRRAKGEEGLFDLLRELGPMTVEDLAQQHTGSSEEIASYLE
                     NLLAVKRIFPTMISGQERLACMDDAARLRDALGVRLPESLPEIYLHRVSYPLRDLFLR
                     YLRAHALVTSEQLAHEFSLGIAIVEEQLQQLREQGLVMNLQQDIWVSDEVFRRLRLRS
                     LQAAREATRPVAATTYARLLLERQGVLPATDGSPALFASTSPGVYEGVDGVMRVIEQL
                     AGVGLPASLWESQILPARVRDYSPEMLDELLATGAVIWSGQKKLGEDDGLVALHLQEY
                     AAESFTPAEANQANSSALQQAIVTVLADGGAWFAQQISQRIRDKIGESVDPSAMQEAL
                     WALVWQGVITSDIWAPLRALTRSSTNTRTSTRRSHRARRGRPVYAQPVSPLVSYNTPK
                     LAGRWSLLQVEPLNDTERMLALAENMLDRYGIISRQAVIAENIPGGFPSMQTLCRSME
                     DSGRIMRGRFVEGLGGAQFAERLTIDRLRDLATQATQTRHYTPVALSANDPANAWGNL
                     LPWPAHPATLIPTRRAGALVVVSGGKLLLYLAQGGKKMLVWQEKEELLAPEIFHALTT
                     ALRREPRLRFTLTEVNDLPVRQTPMFTLLREAGFSSSPQGLDWG"
     gene            complement(25654..26301)
                     /gene="rnt"
                     /locus_tag="ACHKIR_001287"
     CDS             complement(25654..26301)
                     /gene="rnt"
                     /locus_tag="ACHKIR_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HFO9989043.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            complement(26404..26811)
                     /gene="gloA"
                     /locus_tag="ACHKIR_001288"
     CDS             complement(26404..26811)
                     /gene="gloA"
                     /locus_tag="ACHKIR_001288"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFO9989044.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            complement(26892..27989)
                     /gene="nemA"
                     /locus_tag="ACHKIR_001289"
     CDS             complement(26892..27989)
                     /gene="nemA"
                     /locus_tag="ACHKIR_001289"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HFO9989045.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            complement(28026..28625)
                     /gene="nemR"
                     /locus_tag="ACHKIR_001290"
     CDS             complement(28026..28625)
                     /gene="nemR"
                     /locus_tag="ACHKIR_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HFO9989046.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYATYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRDSHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKTIIATPAV"
     gene            28728..28967
                     /gene="ydhL"
                     /locus_tag="ACHKIR_001291"
     CDS             28728..28967
                     /gene="ydhL"
                     /locus_tag="ACHKIR_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HFO9989047.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            29016..29912
                     /gene="ydhF"
                     /locus_tag="ACHKIR_001292"
     CDS             29016..29912
                     /gene="ydhF"
                     /locus_tag="ACHKIR_001292"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFO9989048.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSAEQSLINLATDYLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            29993..30514
                     /gene="sodC"
                     /locus_tag="ACHKIR_001293"
     CDS             29993..30514
                     /gene="sodC"
                     /locus_tag="ACHKIR_001293"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFO9989049.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGAGHLGDLPALVVNNDGKATDAVIAPRLKSLDEVKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(30515..32527)
                     /gene="ydhK"
                     /locus_tag="ACHKIR_001294"
     CDS             complement(30515..32527)
                     /gene="ydhK"
                     /locus_tag="ACHKIR_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFO9989050.1"
                     /translation="MNASSWSLRNLPWFRATLAQWRYALRNTIAMCLALTVAYYLNLD
                     EPYWAMTSAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMAA
                     WLGFCTWACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGI
                     LCGGMMMMILPSSSDATALLTALKNMHARLLEHASLLWQPETTDAIRAAHEGVIGQIL
                     TMNLLRIQAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPCATREIL
                     EQLLTALASSQTDVYTVARIIAPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELQHL
                     QSGVDDHTRLPRTSGLARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGSNALTLA
                     AISCVLYSAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLAT
                     MQLLKLQMPKFAALWGQLIVFMGSFIAVTNPPVYDFADFLNDNLAKIVGVALAWLAFA
                     ILRPGSDARKSRRHIRALRRDFVDQLSRHPTLSESEFESLTYHHVSQLSNSQDALARR
                     WLLRWGVVLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATL
                     EELQRICDSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(32527..33384)
                     /gene="ydhJ"
                     /locus_tag="ACHKIR_001295"
     CDS             complement(32527..33384)
                     /gene="ydhJ"
                     /locus_tag="ACHKIR_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416161.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFO9989051.1"
                     /translation="MSIKTIKYFSTIIVAVVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVDAAQATLKQAQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSNLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            complement(33387..33623)
                     /gene="ydhI"
                     /locus_tag="ACHKIR_001296"
     CDS             complement(33387..33623)
                     /gene="ydhI"
                     /locus_tag="ACHKIR_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HFO9989052.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLSLAILIAW"
     gene            33824..34258
                     /gene="slyA"
                     /locus_tag="ACHKIR_001297"
     CDS             33824..34258
                     /gene="slyA"
                     /locus_tag="ACHKIR_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HFO9989053.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLIKLIAKLEHNIIELQAKG"
     gene            complement(34305..34772)
                     /gene="slyB"
                     /locus_tag="ACHKIR_001298"
     CDS             complement(34305..34772)
                     /gene="slyB"
                     /locus_tag="ACHKIR_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HFO9989054.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            35046..36155
                     /gene="anmK"
                     /locus_tag="ACHKIR_001299"
     CDS             35046..36155
                     /gene="anmK"
                     /locus_tag="ACHKIR_001299"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HFO9989055.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLILSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            36253..36582
                     /gene="mliC"
                     /locus_tag="ACHKIR_001300"
     CDS             36253..36582
                     /gene="mliC"
                     /locus_tag="ACHKIR_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HFO9989056.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLFERMQTDTLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGEEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            36641..37297
                     /gene="pdxH"
                     /locus_tag="ACHKIR_001301"
     CDS             36641..37297
                     /gene="pdxH"
                     /locus_tag="ACHKIR_001301"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HFO9989057.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            37426..38700
                     /gene="tyrS"
                     /locus_tag="ACHKIR_001302"
     CDS             37426..38700
                     /gene="tyrS"
                     /locus_tag="ACHKIR_001302"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFO9989058.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            38759..39622
                     /gene="pdxY"
                     /locus_tag="ACHKIR_001303"
     CDS             38759..39622
                     /gene="pdxY"
                     /locus_tag="ACHKIR_001303"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HFO9989059.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(39666..40271)
                     /gene="gstA"
                     /locus_tag="ACHKIR_001304"
     CDS             complement(39666..40271)
                     /gene="gstA"
                     /locus_tag="ACHKIR_001304"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HFO9989060.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     AVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKSTVRAQLEKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(40377..41879)
                     /gene="dtpA"
                     /locus_tag="ACHKIR_001305"
     CDS             complement(40377..41879)
                     /gene="dtpA"
                     /locus_tag="ACHKIR_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HFO9989061.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHTILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
     gene            complement(42490..43125)
                     /gene="nth"
                     /locus_tag="ACHKIR_001306"
     CDS             complement(42490..43125)
                     /gene="nth"
                     /locus_tag="ACHKIR_001306"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFO9989062.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(43125..43820)
                     /gene="rsxE"
                     /locus_tag="ACHKIR_001307"
     CDS             complement(43125..43820)
                     /gene="rsxE"
                     /locus_tag="ACHKIR_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HFO9989063.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRAEAA
                     AERALPNGETGNV"
     gene            complement(43824..44444)
                     /gene="rsxG"
                     /locus_tag="ACHKIR_001308"
     CDS             complement(43824..44444)
                     /gene="rsxG"
                     /locus_tag="ACHKIR_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HFO9989064.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLETTAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDANWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(44448..45506)
                     /gene="rsxD"
                     /locus_tag="ACHKIR_001309"
     CDS             complement(44448..45506)
                     /gene="rsxD"
                     /locus_tag="ACHKIR_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HFO9989065.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTTSGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGMLAGV
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLASPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLMFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(45507..47729)
                     /gene="rsxC"
                     /locus_tag="ACHKIR_001310"
     CDS             complement(45507..47729)
                     /gene="rsxC"
                     /locus_tag="ACHKIR_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HFO9989066.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSHDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIARA
                     KARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEQQVDPRKAAVE
                     AAIARAKACKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQVDP
                     RKAAVEAAIARAKARKLEQQQANAEPEEQIDPRKAAVAAAIARVQAKKAAQQKVVNED
                     "
     gene            complement(47722..48300)
                     /gene="rsxB"
                     /locus_tag="ACHKIR_001311"
     CDS             complement(47722..48300)
                     /gene="rsxB"
                     /locus_tag="ACHKIR_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HFO9989067.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISYNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            complement(48300..48881)
                     /gene="rsxA"
                     /locus_tag="ACHKIR_001312"
     CDS             complement(48300..48881)
                     /gene="rsxA"
                     /locus_tag="ACHKIR_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HFO9989068.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFFQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            complement(48958..49398)
                     /gene="ydgK"
                     /locus_tag="ACHKIR_001313"
     CDS             complement(48958..49398)
                     /gene="ydgK"
                     /locus_tag="ACHKIR_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HFO9989069.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGEQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            complement(49484..49699)
                     /gene="ydgT"
                     /locus_tag="ACHKIR_001314"
     CDS             complement(49484..49699)
                     /gene="ydgT"
                     /locus_tag="ACHKIR_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HFO9989070.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            complement(49972..50097)
                     /gene="blr"
                     /locus_tag="ACHKIR_001315"
     CDS             complement(49972..50097)
                     /gene="blr"
                     /locus_tag="ACHKIR_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HFO9989071.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSTTVQHK"
     gene            50340..51380
                     /gene="ydgJ"
                     /locus_tag="ACHKIR_001316"
     CDS             50340..51380
                     /gene="ydgJ"
                     /locus_tag="ACHKIR_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFO9989072.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELEALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEGWGYDMRD
                     GVLTRVEGEERIEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            complement(51416..52417)
                     /gene="add"
                     /locus_tag="ACHKIR_001317"
     CDS             complement(51416..52417)
                     /gene="add"
                     /locus_tag="ACHKIR_001317"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFO9989073.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(52521..53693)
                     /gene="malY"
                     /locus_tag="ACHKIR_001318"
     CDS             complement(52521..53693)
                     /gene="malY"
                     /locus_tag="ACHKIR_001318"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HFO9989074.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFASAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQSVVYGPSVIYMVSE
                     LIRQWSEKGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKRADGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHGVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYIADKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            complement(53703..55295)
                     /gene="malX"
                     /locus_tag="ACHKIR_001319"
     CDS             complement(53703..55295)
                     /gene="malX"
                     /locus_tag="ACHKIR_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HFO9989075.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            55470..56498
                     /gene="malI"
                     /locus_tag="ACHKIR_001320"
     CDS             55470..56498
                     /gene="malI"
                     /locus_tag="ACHKIR_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HFO9989076.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECSSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            56610..57377
                     /gene="hdhA"
                     /locus_tag="ACHKIR_001321"
     CDS             56610..57377
                     /gene="hdhA"
                     /locus_tag="ACHKIR_001321"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HFO9989077.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFACRCDITSEQELSALADFAVSKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            57598..58188
                     /gene="uidR"
                     /locus_tag="ACHKIR_001322"
     CDS             57598..58188
                     /gene="uidR"
                     /locus_tag="ACHKIR_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HFO9989078.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLSREASFAQGLRAMIGGILTAS"
     gene            58575..60386
                     /gene="uidA"
                     /locus_tag="ACHKIR_001323"
     CDS             58575..60386
                     /gene="uidA"
                     /locus_tag="ACHKIR_001323"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFO9989079.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESVLQEIRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KDEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            60383..>61734
                     /gene="uidB"
                     /locus_tag="ACHKIR_001324"
     CDS             60383..>61734
                     /gene="uidB"
                     /locus_tag="ACHKIR_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HFO9989080.1"
                     /translation="MNQQLSWRAIVGYSLGDVANNFAFAMGALFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHSSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQAQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQQ
                     "
BASE COUNT        15491 a        15279 c        16111 g        14853 t
ORIGIN      
        1 ttttctccta accataaagg attagtttat cagcgttaaa tcttttctca tacactattg
       61 ggttagggaa aatatctggt gttgaggaca gagtcagcag cgtattagtt gagtgcctaa
      121 tatcaggctg ctgaattgtc agcactgtgt ttgcgaagag gatgcgaaat aaatgaaccc
      181 ggttgatcgt ccactattaa atattggctt aacgcgatta gaattcttgc gtatatcagg
      241 aaaaggcctt gctggtttaa caattgcccc tgcgttactg tcacttttgg gttgcaaaca
      301 ggaagatatt gatagcggca cagtaggctt gataaataca cccaaagggg tgctggtgac
      361 ccatcgtgcg cgatgcacgg gttgccaccg ctgtgaaatc tcttgtacca acttcaacga
      421 tggctcagta gggacattct tctcccgtat caaaatccat cgcaattatt tctttggcga
      481 taacggggtt ggctctggtg gcggcctgta tggcgatctc aactataccg cggacacctg
      541 ccgtcaatgc aaagagccgc aatgcatgaa cgtctgcccg attggtgcga ttacctggca
      601 gcagaaagaa ggctgtatta ccgtcgatca taaacgttgt attggctgta gcgcctgtac
      661 tacggcatgc ccgtggatga tggccaccgt aaataccgaa agtaaaaaat cctcgaaatg
      721 tgtgttatgc ggtgaatgcg caaacgcctg cccaacaggg gcgttaaaaa ttatcgagtg
      781 gaaagatatt actgtgtgaa tcttgcaaag gaaaaaatca tggctaacgg ttggacaggt
      841 aatatattaa gagtcaatct cacgacagga aatattaccc tcgaagattc cagtaagttt
      901 aaaagttttg tcggtggcat gggcttcggc tacaaaatta tgtatgacga agtaccgcca
      961 ggcacgaaac ctttcgatga agcgaataaa ttagtttttg ctaccggccc attaactgga
     1021 tctggtgccc cctgtagttc tcgcgtaaat atcacctcac tttctacttt taccaaagga
     1081 aatttagtcg tcgatgccca tatggggggc ttttttgcag cgcaaatgaa attcgctgga
     1141 tacgacgtca ttattatcga agggaaagcg aaatcaccgg tatggctgaa gattaaagat
     1201 gacaaagtta gccttgaaaa agccgatttc ttatggggaa aagggacgcg cgcaacgacg
     1261 gaagaaattt gtcgcttgac cagtccggaa acctgtgtgg cggctattgg tcaggctggg
     1321 gaaaaccttg ttcctctctc tggcatgttg aatagccgta accacagcgg tggtgcggga
     1381 actggcgcaa taatgggttc gaaaaacctg aaagcgattg cggttgaagg gacgaaaggg
     1441 gtcaacattg ccgatcgtca ggagatgaag cgtctcaacg attacatgat gactgaactt
     1501 atcggtgcga ataacaacca tgtcgtgcca agtacgccgc aatcgtgggc agagtattca
     1561 gatcccaagt cacgttggac tgcacgtaaa gggttgtttt ggggcgcggc tgaaggtggt
     1621 ccgattgaaa cgggtgagat tccgccaggc aatcagaaca cggtcggctt tcgtacctat
     1681 aaatccgttt ttgacttagg tccggcggca gagaaataca cagtaaaaat gagtggctgc
     1741 cactcttgcc cgatccgttg tatgacccaa atgaatattc ctcgggtgaa agagtttggc
     1801 gtgcccagca caggtggtaa cacttgtgta gcaaactttg tccataccac catcttcccg
     1861 aacggtccga aagattttga agataaagat gatggtcgtg tgattggtaa cctggttggt
     1921 ctgaatctgt tcgatgacta cggcctatgg tgtaactatg gtcagcttca tcgcgacttt
     1981 acctattgtt acagcaaagg ggtgttcaag cgtgttctgc ctgctgaaga gtatgcggaa
     2041 attcgctggg atcaactgga agcgggtgac gttaacttca ttaaagattt ttactaccgt
     2101 ctggcgcatc gcgtgggtga gctgagtcac ctggctgatg gttcatatgc catcgcagaa
     2161 cgctggaatt tgggtgaaga gtactggggc tacgcgaaaa ataaactctg gtcgccgttt
     2221 ggctatccag ttcaccacgc caatgaagcg tcagcgcagg tcggctccat tgttaactgt
     2281 atgttcaacc gtgactgcat gacgcatacc catatcaact ttattggttc cggcttgcca
     2341 ttgaaactgc aacgtgaagt ggcgaaagaa ctttttggtt ctgaagatgc ttacgatgaa
     2401 accaaaaact acacgccaat caatgacgca aaaattaaat atgctaagtg gtcgctgttg
     2461 cgggtctgtt tgcataacgc cgtcaccctg tgcaactggg tctggccaat gaccgtttcg
     2521 ccgctgaaaa gccgtaatta tcggggcgat ctggcgcttg aagccaaatt cttcaaagcg
     2581 atcactggcg aagatatgac tcaggaaaaa ttagatttag ctgcagagcg tatctttacg
     2641 ttgcatcgcg cctacacggt aaaactgatg caaactaaag atatgcgtaa tgaacacgat
     2701 cttatctgtt cctgggtatt cgacaaggat ccgcagatcc cggtctttac tgaaggtaca
     2761 gacaaaatgg accgtgacga tatgcatgct tcgctcacga tgttctacaa agaaatgggc
     2821 tgggacccac agcttggttg tccgacccgc aaaacattgc agcgtctggg gctggaagat
     2881 attgccgccg atctggcagc acacaatcta ctgcctgcgt aaggaatggg tgagatgaac
     2941 catcaggatg aattgccgtt agcgaaagtc agcgaagttg atgaggcgaa gcggcaatgg
     3001 ttgcagggga tgcgtcaccc cgttgatacg gtaaccgaac cagaacctgc tgaaatcctg
     3061 gcagagttca ttcgtcagca ttctgcggct gggcagctgg tggcgcgcgc cgtgtttctc
     3121 tcgccgccct attcggttgc agaagaggaa ctgtcagttt tgctggaaaa cattaagcag
     3181 aacggagacc acgcggatat cgcgtgtatg acgggaagcc aggatgatta ctattactca
     3241 acgcaggcca tgagtgaaaa ctacgcggcg atgtctttgc aagttgtgga gcaggacatt
     3301 tgtcgcgcca ttgcacacgc cgttcgtttt gagtgccaga cgtatcctcg tccttacaaa
     3361 gtcgccatgc tgatgcaagc gccatattac ttccaggaag cgcagatcga agccgcgatc
     3421 gccgcgatgg atattgcacc tgaatatgct gatatacggc aggtggagtc atcgacggcg
     3481 gtgttgtact tgtttagcga gcgatttatg acctatggca aggcgtatgg cttatgcgaa
     3541 tggttcgagg tagagcagtt tcagaacccc tgaaatttca gggagatgtc atggataggg
     3601 tggacagtag cagccactgc attaggggat aatcagatgt ccttcactcg acgcaaattt
     3661 gttctgggga tgggaacagt aatctttttt actggttctg cgtcttctct gttagcgaac
     3721 acgatgcaag aaaagaaagt tcggtatgcc atgattcatg acgagtcacg gtgcaatggc
     3781 tgtaatattt gcgcccgcgc atgccgtaaa actaaccacg tccccgctca gggaagccgt
     3841 ttatcgatag cacacattcc agtcaccgat aacgacaacg aaacgcagta tcactttttt
     3901 cgccagtcat gccagcactg tgaagatgct ccttgcatcg acgtttgccc gacaggggcg
     3961 tcgtggcgtg atgagcaggg gatcgtgcgg gtagaaaaat cgcagtgcat cggctgcagt
     4021 tattgcattg gcgcttgtcc ttaccaggtt cgctacctta accctgtcac taaagtggca
     4081 gataaatgtg atttctgcgc tgagtcccga ctggcaaaag gctttccgcc catttgcgtc
     4141 agcgcctgtc cggaacacgc attgatcttt ggtcgcgaag atagcccaga aattcaggcg
     4201 tggttgcagg aaaataaata ctatcaatat cagctacctg gtgccgggaa accgcacctg
     4261 tatcgccggt tcggtcaaca tttgattaaa aaggaaaatg tatgaacccg tcgcaacatg
     4321 ctgaacagtt tcagagccag ttggcgaact atgtgccact gttcactccc caattttggc
     4381 cggtgtggtt gatcattgcc ggactattgc tggtagggat gtggttggtg ctggggctgc
     4441 atgccttgct tcgtgctcgt ggcgtgaaga aatcagccaa cgaccatggt gagaaggttt
     4501 atctttacag caaagcggtc agattatggc actggtcgaa tgcgttactc tttgtattgt
     4561 tgctggcaag tgggctgata aatcactttg cgctggtggg cgcaactacg gttaaaagtc
     4621 tggttgcggt gcatgaagtt tgcggatttt tgttactggc atgctggctc ggctttgttc
     4681 tgatcaatgc cgttgggggt aatggtcacc actatcgcat tcgtcgtcag gggtggctgg
     4741 agcgagcggc aaaacaaacg cgattctatt tgtttggcat tatgcagggg gaagaacatc
     4801 ctttcccggc aacaacccag tctaaattta atcccttaca gcaggtcgcc tatgttggtg
     4861 tcatgtatgg attgctgcca ttgttactat tgacggggct gctgtgtctc tatccgcaag
     4921 ccgtgggaga tatgtttcct ggcgtaagat actggttatt gcaggcgcat tttgctctgg
     4981 catttataag cctctttttt atcttcggtc atctttatct ttgcaccacg gggcgtacgc
     5041 cacacgaaac ctttaaaagc atggtcgatg gctatcaccg gcactaacga atgatcatta
     5101 cacgagcaga cctgcgagaa tggcgtatcg gagcggtgat gtatcgctgg tttctgcgcc
     5161 atttcccacg cggcggtagt tatgccgata ttcatcatgc actgattgag gaaggatata
     5221 cggactgggc ggagagtctg gttgagtatg cgtggaaaaa atggctggcg gatgaaaact
     5281 tcgcgcatca ggaagtttct tctatgcaaa aactggccac agatcccgga gagagagcat
     5341 tttgcagtca gtttgcccgc agcgacgatc atgctcgcat cggctgttgt gaggataatg
     5401 ccagaatcgc aaccgctggg tatgccgcgc aaattgccag tatgggttat agcgtgcgga
     5461 ttggaagtgt cggctttaac agtcacattg gcagctccgg tgaacgcgca cgggttgctg
     5521 taaccggtaa ttcttcgcgg ataagcagcg ctggagatag cagtcggatt gcgaataccg
     5581 gaatgcgagt acgcgtttgt acattgggtg aacgatgtca tgtcgccagt aatggtgacc
     5641 tggtgcaaat cgccagcttt ggcgcaaacg ccagaattgc caacagcggc gacaatgtac
     5701 acatcattgc cagcggtgaa gattcaacaa tagtgagcac gggcgtggtc gactcaatta
     5761 ttctcgggcc agggggaagc gccgcactgg catatcatga cggcgaaaga gttcgcttcg
     5821 ccgtcgccat tgaaggtgaa aacaatattc gagccggagt ccgctatcgc cttaatgaac
     5881 agcatcagtt tgttgagtgc tgatagcttt acatttgatt aaacgaaaga cgccgacgag
     5941 cacgggatgc aggctttacg accgcctgag ctatcgcctc accaatttct gcacatgccg
     6001 taagtccctg tacgaatggc tggtcgtgca tcaaccaggg caacgcgccg aacgctacgc
     6061 gcccacgaat ttcttcgggt tgctgtagcg tataatcttc gccaacatca gggatttcat
     6121 cccctgtttt ctgtaattgt tcgcgtagtc cagggaaagg aatatctttc actttaagcg
     6181 gacgctgccc acgggcatca ataaaaacgt caaacgagta gctattgtct tccgttttca
     6241 ggacggtgcg cgactcatta atttccattt cgtagtcttc accgagggcg agaatatgaa
     6301 tgattcccgc ttcacgtaag gccagtagtc gacgaataga ctctgaaggg attgccgcat
     6361 aattatcgat gaaaacccgg gcaaggcctt tactgaaccg tttatggtcg tgttcattca
     6421 gatgaggaac aatttcctgt acggcttcat gcaggcgcag aatgacataa cgccagggaa
     6481 cagtatgttt ttctcgttta ttgcgttcga cttcctggag atttttttct gcccagtcga
     6541 atgggtcgcg ttgtttgcgc tcggcaaacc aggcttgagc aaaggaatcg acattcaggc
     6601 tttctaaggc tatgcgttgg ctccagtctg gatcagcaaa tttgatttcc tctactatca
     6661 atctaaatac ccgatccaaa aggcccactt cgcctttttg aatctcagca tttaatgctt
     6721 gatcggtgac gatgtgtaag ggctcgtagg gaatagggca atagaaatcg gcttcgggta
     6781 aaatacccgt acgcgacatc aacgtaatat ttagcttttc acttgcgtta tcgcggtgaa
     6841 aaacgacgtg ttgtttatca tcttcaatga acgaaccatg ctgaatagcc actgccattg
     6901 ccgcatccag tccgctcaag gatgttccca taatacccac gttacacgca tcgacctttg
     6961 cttccatcag gcctgaccac gggctgggaa aatacgttcg ggttgcttct tcttcatcag
     7021 gccagacgtg acccgtggcg atcaccgcta aatcaaacgt ctcgctgggt aaatcctgat
     7081 ttgtagcgag catgacgcca gcatttgtaa tttgcagatc ggtaacctgg catgattcat
     7141 aaacagccac tgcaaatttt tgctgtcgtg cctggtctac taatcgtaaa aattgatcgc
     7201 ggaaatattc gcccagcaga attcgcggta aaaactgacg atcgtgcaag gtttcttttt
     7261 taacgccata acgctggaga tggctggctt cttgcttttg tagccattcg agatacgtac
     7321 aataaatcgg cggtatttca atactggcaa tatttgccag catcattttt gagttttcct
     7381 catcactgta tggcatcccg acaccggctt catcagcctg ctcgaagata gaaatagaaa
     7441 gtggagtttg ttgctgtaga agcgagaata aggtgtagat ccccgtaggc ccggcaccca
     7501 caatagcaat ttttttcatt gttacttacc tgtacgcatt ctttcgaatt tagtgttatc
     7561 agataagttt ggttgagatt tttagaattt gagaaatttc agacagggat gaaaagggcg
     7621 ctaaccgccc ttgattaaat tctgtcaggc gagttttgcc tgattgattt gactaagtgg
     7681 ctcattgaca tcgaaaactt tggcgacaac ttcctcaacg ccgagatttt tcagtcgggc
     7741 agtatgtttt tccagatagg caagggcgct tttttcatta gtaaaaaggt agatcccacc
     7801 ggcttcgtgg ttcttttcac tttctgtcca taccttccac agaaaaccag gttcctgatt
     7861 aatcgactca gcaagcggtt taagctgctc agccattgcg tcgccaaatg ggccattgaa
     7921 agaaaaatga agttgtaaca aagttgccat agcgattctc cttctgataa cgcatacgaa
     7981 gacagtgtaa atcttttaac gtattggata tagagaagaa acagagggaa aaaggagaag
     8041 accagattaa gaaaatctgg tgcgtccgag tggactcgaa ccaccgaccc ccaccatgtc
     8101 aaggtggtgc tctaaccaac tgagctacgg acgcaggatg gtgcgttcaa ttggactcga
     8161 accaacgacc cccaccatgt caaggtggtg ctctaaccaa ctgagctatg aacgcaacgt
     8221 tgtaggtgac aacggggacg aatattagcg gcagagtggg aaggtggcaa gaggcaaaac
     8281 gtaattttct gcgctatttc gaccgtttgc agagctttta agcaaattgg ctatattttg
     8341 ttgatttgca agggcgattt ttattcagga tcgcatttac atcagataca acccaataat
     8401 ccgtgtatca gagtttaaac agagttgttt gccaccatct caaaatctgt cgtggataaa
     8461 tcctccgtag cctttacact taggtagcaa taaaggcaca cagatgcagc aaaggaataa
     8521 atcatgggat ctgatgcgaa aaacttgatg agcgacggga acgtgcaaat tgttaagacc
     8581 ggcgaggtca ttggcgcgac gcaacttact gaaggtgagt taattgttga agctggcgga
     8641 agagccgaaa ataccgtggt cacgggggct ggctggttga aagtggcaac cggtgggatc
     8701 gccaaatgca cacagtacgg caacaatggc acgctatcgg tcagcgacgg tgccattgcc
     8761 acagatattg ttcagtccga gggaggcgca attagtctct ctacgctcgc tacggttaat
     8821 ggccgccatc ccgaaggtga attcagcgtt gataaaggtt atgcctgcgg tttgttgctg
     8881 gaaaatggcg gtaacctgcg tgtactggaa ggccatcgcg cggaaaaaat tattctcgat
     8941 caagagggtg gcctgttggt caatgggaca acctcagcgg tcgtggtaga tgaaggtggt
     9001 gaattgttgg tgtatccagg tggggaagcc agcaattgtg agattaatca gggcggcgtt
     9061 tttatgctgg cggggaaagc cagtgataca ttgcttgctg gtggcaccat gaataatctc
     9121 ggtggtgaag actctgacac tattgttgag aatggatcca cttatcgtct ggggacggat
     9181 ggtcttcagc tctacagttc cggtaagacg caaaacctgt ccgttaatgt gggtggtcgg
     9241 gctgaagtgc atgccggtac gctggaaaat gcggtaatac aaggtgggac agtcatcctg
     9301 ttgtcaccca ccagcgcgga cgaaaatttt gtcgtagagg aagatcgcgc accggttgaa
     9361 ctgaccggta gtgttgcatt actggacggc gcttcaatga ttattggcta tggcgcagat
     9421 ctgcaacaat caacgattac tgtacagcag ggcggtgtat tgattctcga cggcagtacg
     9481 gtaaaaggtg acagtgtcac tttcagtgtt ggtagcatca atctaaatgg cggaaaactg
     9541 tggctaatca ctggtgcggc aacgcatgtg caactgaaag tgaaacgcct gcgcggagag
     9601 ggagcgattt gcctgcaaac cagtgcgaaa gaaatttcac ctgacttcat caatgtgaaa
     9661 ggggaagtta ctggtgatat acacgttgag ataacagatg ccagtcggca aactctgtgt
     9721 aacgcactga aactacagcc agacgaagac gggattggcg caacgctcca gcctgcgtaa
     9781 aaacacaggc gcaacccgtg aaggttgcgc cttgtcttta ctagcgagct gctcgttgca
     9841 gaatgatggc tgacggcaga cgttgcagga accgcatacg cagcatcatc ataatggctg
     9901 ccgacgtcag gccaataata aagcctatcc agaagcctgc tggccccata ggttcaacga
     9961 ccagatcggt cagtgccaga atatagccgc ttggcaagcc cagcacccag taagccgtaa
    10021 aggtaatata gaaaatggaa cgcgtatctt tatatccacg caaaatccca ctgccaatca
    10081 cctggattga gtcagaaatc tgatataccg ccgccagcaa catcaaatgc gcagccagcg
    10141 ttacaacctc gggattgtcg ttgtacaaca gggcgatttg ctcccgcagt gaaaccgtga
    10201 aaatggccgt cagggttgcc atacagacac ccaccataag cccggtccgc gcagcggttt
    10261 gcgcatccag cgttgagccc tgacccagac gataacctac gcggatggtt accgctgccg
    10321 ccagcgacat gggaagtacg aacatcagtg aactaaagtt cagggcaatc tgatgtcctg
    10381 cgacatcaac aataccgagc ggagacacta acagagccac gacggcaaac agcgttactt
    10441 caaagaacag cgccagcgca attggcaaac cgagttgaat cagtcgtttc ataaccgcag
    10501 gatcgggctt cgcagtgcct ttttcgttac gaatatcgcg catcgagcgg gcgcgtttaa
    10561 tgtaagaaac catggcaagg aacatgaccc aatacaccgc agcagtagcc acgccacaac
    10621 caacgccacc gagctcaggc ataccgaaat gaccgtaaat aaagatatag ttcaccggaa
    10681 tgttcaccag caggccgata aagcccatca ccatacccgg cttggttttt gccagacctt
    10741 cacactggtt acgggcaacc tggaagaaaa gatagcccgg cgcgccccat agcaacgcac
    10801 gcagataacc cacggctttg tccgccagag ccggatcgat gttttccatg gagcggataa
    10861 tgtaacctgc attccacagc accagcataa tgagaacgga aacaaaaccc gccagccaga
    10921 aaccttgtcg cacctgatgc gcaatgcgct cacgtcgacc ggaaccattt aattgcgcga
    10981 taaccggcgt taatgccagc agcagtccgt gaccaaagag gatcgccgga agccagatag
    11041 aagtaccgat agcgaccgcc gccatgtcgg tggcactata gccgcccgcc atcacggtat
    11101 cgacaaaacc catcgcagtt tgggcgattt gcgcgagaat caccgggatt gctaatgcta
    11161 ataacagacg cgcttcactg atatacttct gcacgtgaac accttttatt tgtagttata
    11221 tgagagacta aaaagccgcc tgaatgggca gcaaaaagaa gaagcagggg aaattcagtc
    11281 tattgtagcg aggatttatt aattctccag tgaaaaaata gtcattctta ccccttcact
    11341 ggtaagggcg tttgccacct gctattgtgc tgaacagaat gtcttaactg atttcaggag
    11401 ttgtaagtat gtttacgggg attgtacagg gcaccgcaaa actggtgtcg attgacgaga
    11461 aaccaaattt tcgcacacat gtggtggagt tacccgacca catgctggac ggtctggaaa
    11521 ccggtgcttc cgtggcgcat aacggttgct gcctgaccgt gacggaaatt aacggcaacc
    11581 atgtcagttt tgacctgatg aaagaaacgt tacgcattac caatcttggc gatttaaaag
    11641 tgggggattg ggtaaacgtt gagcgtgcgg cgaaattcag tgatgaaatt ggcggacact
    11701 taatgtcagg tcatattatg accaccgctg aagtggcgaa aatattaacc tcggaaaata
    11761 atcgccagat ctggtttaaa gtccaggata gtcagttgat gaaatatatt ctgtacaaag
    11821 gatttattgg catcgacggt attagcctga ccgtcggtga agtcacgcca acgcgttttt
    11881 gcgtccattt aattccggaa acactggaac gcacgactct tgggaagaaa aaacttggcg
    11941 cacgcgtcaa cattgaaatc gatccacaaa ctcaggcagt ggtagatacg gtagaacgag
    12001 tgctggcggc acgagaaaat gccatgaatc aaccaggtac agaagcctga tctgaaatcg
    12061 cccccggaga gggggcgata gaatagcctt tagcgagcca ctcgaaggcc gttttcaaca
    12121 ccgcgtgaga acacgacctg ccagagctga atatcacggg cgcggaaagc acccgcacag
    12181 gcattcagat aataggtaaa catccgttta aagcgttcac tgtagttatc cgcaatttct
    12241 ggccatgcgg cgaggaatcg ttcataccac gccatcaacg tagtatcgta atcagcaccg
    12301 aagttatgcc agtcttccat cacaaagtgg ggttcgctgg actgagcaat ctggcgtaca
    12361 gagggcaggc aaccgttcgg aaaaatatat ttattaatcc agggatcaac attcagatcg
    12421 gtttttttcg aaccgatagt atggagcagg aatatgcctt ccggtttcaa attacgatcc
    12481 accaccgcaa aataggtatc gtaatttttc ggtccgacgt gctcgaacat ccccacagaa
    12541 acaatacgat caaactggtc gttcaggtca cgataatctt gcagcaaaat ggtgacatcc
    12601 aggccttcac agcgttcctg agccattttt tgctgttcgg cagaaatggt gacgccgacc
    12661 acgcttacgt cataattaga cgccatgtag tgcgccagtc caccccagcc acagccaata
    12721 tccagtacgc gcatccctgg ttttaactgc aatttttcac aaatcatttt gagcttcgcc
    12781 tgctgggctg attccagatt atcggcatct ttccagtaag cacaggaata ttgcatgaag
    12841 ggatcaagca tgcggctgaa caagtcatta cccaaatcgt aatgctcttt gccgactatc
    12901 caggcacgtt ttttactctg cagattgaag agacgagcgc cggcaatacg cagcgtgtct
    12961 ttgaaatgat gggggagttg gttctcgaga cctgcgcgta agactttgct aaaaaacata
    13021 tccagtcggt cacattccca ccagccatcc atataacttt cgcctaaccc caacgatcct
    13081 tcctgcagaa cgcgtttaaa aaaatcgggg tttttcacac gaatatccgc cggggcagaa
    13141 ccgttaatgg ctataccggc acggctaagt aattcgttgg cgatacggta ccagttgtca
    13201 tccggtacac tgacttcttc tatacacgat gaactcatag tttctccatc actggtcggt
    13261 gatcagaacc ttaaaacagc gtagacgctt tttgggcttt gtgagaaatc tcgcagaaaa
    13321 aaccgcgagt ctataatccg acgtagaaca gagggaaagg aggaaccctt gccgaaatga
    13381 ccatccatag tgagtcggga acgatcctgt ccccgtaaaa acataatatt tatcgtagct
    13441 ttaacaactc gaaattagta taggccgcta atttcaatca tacaacaaaa ttttgttagc
    13501 agcctaagta taatcatatc gatatagatc agtgtgattc gctatgagcg acttcggcat
    13561 tgccatgatt ctgacagtct acttcttcac aacgttgcat catgtaaccc agagcgacca
    13621 gcactactgt tgataacatc acgctggtgg tggtgagcaa tggcgtgctg atactgatca
    13681 gccaggaaac taccagactt gcgaggaagc acagacccag ttgtagagtg ttctgcaacg
    13741 ctgcggcgcg accggttgca tgtgggaagg gacgcagcgc ctgggcgaca acaatcgggt
    13801 agatcgcgcc attggcaatc gccatcacac agaatgggat caggatttcg actagcgaca
    13861 catggctaat gaagcccgca gcccaggtcg caatgacgct gacagcaaac agtaccagca
    13921 accacggtaa taactgcttg ccttgccatt tctgcagtgc ggcgcgacag ccataaccac
    13981 caatcagaaa cgcgatagtt tgcgggacat aacttaaacc aataactgcc gggctgtagc
    14041 ccatttcact aaggatgaac ggtgagccgg tcagccatgc aaaaaaactg gctgaacagg
    14101 ccgcatatat cagcacgttg ccgcgatagg ttttagaacg tagcaggtcg gtaaaggtca
    14161 gaccatcctg actattgtta cgggccttcg tcgtgggttt gagccagaaa ataggcagaa
    14221 tcagcaccac ggtaatggca aacagggtgg cgaaaatcgc ctgccaggaa aaatggacca
    14281 gcagccagct tcctaacaga ggagccagtg ccggagatag acccaccagc ggcatgatgg
    14341 tcgcaaaaat acggttaact ttctgtgaag gataataatc cgtcactaac gcttgccaga
    14401 taactgccgc ggcgcagaca cccacagcct gtacaaaacg caataccagc agcgtagcgg
    14461 cgttttctac ccacagcatc cccagactac ctaacgcaaa aattgtcagg ccgattaata
    14521 ataccggttt acgaccataa cggtcggaga gcggccccca cagaagctgg gctgcggcaa
    14581 aaccggcaag gaacagacta aggctggcac tgacagcaga cgcaggcgtt tgcaggtcgg
    14641 cctgtatggc ggcgaaagca ggcagataca tatcggttgc cagaaaaccg agtacgctca
    14701 aacccgccag ccagactaaa aatcttttcc caggttgcat gtatattctc taaatctctc
    14761 aattaaacac cgccgcaaag tgtagggagt gaaattagtg ttgtgaaacg gtaatatttg
    14821 atgactggtt tcaaaaattt tgcaggcaga ctatgtggtc agaatattca ctcgaagttg
    14881 ttgatgcggt agcgcgtaat ggtagtttta gcgctgcggc acaggagttg catcgcgttc
    14941 cttctgcggt cagctatacc gtgcgtcagc tggaagagtg gctggcggtg ccgctgtttg
    15001 aacgacgtca ccgtgatgtg gaactgaccg ctgccggggc gtggtttctc aaagaagggc
    15061 gctctgttgt caaaaaaatg cagatcaccc ggcagcaatg tcagcagata gcgaacggct
    15121 ggcgcggtca gttagctatc gcagtggata atattgtcag gccagaacgt acacggcaga
    15181 tgatcgttga tttttatcgc cattttgatg atgtcgaact tcttgtcttt caggaagtgt
    15241 tcaacggtgt ctgggatgcg ctttccgacg ggcgcgtgga actggcgatt ggcgcaacac
    15301 gcgcgattcc ggtaggcggt cgttatgcct tccgcgatat ggggatgcta agctggagtt
    15361 gcgttgttgc cagccaccac ccgctggcgt tgatggatgg cccgttcagc gatgatacgt
    15421 tgcgcaactg gccgtcgttg gtgcgcgaag acacctcgcg aacgctacct aaacgtatta
    15481 cctggttgct ggataaccaa aaaagacttg tcgtgcctga ctgggaatca tcggcaacct
    15541 gtatctcggc aggattatgc atagggatgg tgccaacaca tttcgccaaa ccgtggctta
    15601 atgaggggaa gtgggtagca ctagaactgg aaaacccctt cccggattcg gcatgttgtc
    15661 tgacatggca gcaaaatgat atgtcgcctg cgttaacctg gttgcttgag tatctcggcg
    15721 atagtgagac gctgaataag gagtggctgc gggagccgga agagactccc gcaacgggtg
    15781 attaacgacg atagtcgcgg aacgggccgt cagccacgga gcggcgttca atcaagcgcg
    15841 gatgcacttc aatggactgc ggttcttcgc gtttattgac gatacgatcc aacagcatgt
    15901 tgaacgccgt ttcgcccagc gaatcttttg gctgatggat cgtggtcagc gctggcgtaa
    15961 aatagcgggc gttgcgcacg ttatcataac cgatcagcga aacatcctgc ggcacgcgca
    16021 gacccatttc atcagcagca caaagtgcgc ccattgccat gatatcgcca ccacagaaga
    16081 cggcagtagg gcgatgcggt tgcgacagta tttgctgcat ggcgcgataa ccggattcag
    16141 gttcaaagtc accctgtaca atccagcttt ccggcacttt gatcatcgct tcttccatcg
    16201 ccttcataaa accggcaagg cggcctgcgc cggtgttacg ttccagcggg ccagggataa
    16261 cgccgatttc gcgatgaccg cgttcaatca gataacgccc ggccatgtag ccgccttcga
    16321 atgcgttatc aatgaccgca tcggtgaagt cagcttttgc ttcaccccag tccatcacca
    16381 ccattgggat atggcgatac tcttccagca tcgccagcaa cggctctggg tactcagaac
    16441 acatcaccag cagaccatcg acgcgttttt gcgccatcat cgacagataa gcccgctgtt
    16501 tctcaagatt gttccacgca ttgcccagaa tcagggtgta acctttctgg aagcaatttt
    16561 tttcaaccgc ttcaatgatc tcggcaaaat aggccgcttc gctgctggtc gccagcaaac
    16621 cgatagactt ggtgtggtta accttcaggc tacgcgccac cgcgctaggg gagtagtgta
    16681 attctttaat cgctgcccac acggcgttgc gcgtttcttc agcgacgaaa cgtgttttgt
    16741 tgatcacgtg tgacacagtt gtagtggaaa cgtttgctcg tttcgctaca tcttttattg
    16801 ttgccattcc atttcactcc agaccctaac ttcagctcct gcaaaatcgc aaggtaaacg
    16861 tttgccttta cacaccttac gcacaacaaa agtgttgcgg tacgccggaa aaacgacacg
    16921 gagcgtcagg aaggggtcaa tggccagtac gcaaatagtg taagcgtgga attttggccg
    16981 atcttgacga aaaggggaag tggtaaaaac acttatcatt ataaatgacg ggagattttt
    17041 tcatcacagt gtgtaaaaat gcgtaatatc actttttgtg tggtaataaa aaaggagaaa
    17101 acttgatgag caccgacctt aaattttcac tggtaacaac gattatcgtc ctcggtttga
    17161 tcgtggtcgt gggtttgact gccgcgctgc actgatttcc tctcgaggga gcgcgttctc
    17221 cctcgcatct ccttcctgtt tgttagcaaa atggcaaaag tgttttctct ttttgttatt
    17281 ttcatttttt tgtgattgct gtcatgcttt cgccacttta atgaatatcg cgtaatgacg
    17341 cgaagaggtg gagataaggc atgaaaatta actatccgtt gctggcgctg gcgattggcg
    17401 cgtttggtat cgggacaacg gagttctcgc caatgggctt gttgcccgtc attgcgcgcg
    17461 gtgtggatgt ctcgattccc gctgccggaa tgttaatcag tgcttatgca gttggcgtaa
    17521 tggttggcgc gccgctgatg acgcttctac tttctcatcg tgcccgccgc agtgcgttga
    17581 ttttcctgat ggcgattttc acgcttggca acgtactttc cgccatcgcg ccggattata
    17641 tgaccctgat gctttcacgc attttgacca gcctgaatca cggggcattt tttggtttgg
    17701 gttcagtcgt ggccgcaagc gtggtgccaa aacataaaca ggccagcgca gttgccacta
    17761 tgtttatggg gttaaccctg gcaaatatcg gtggcgtgcc ggcggcgacc tggttgggtg
    17821 aaaccatcgg ctggcggatg tcatttctgg caacggcggg gctgggagtg atttcaatgg
    17881 taagtctgtt cttctcatta cctaaaggtg gtgcaggggc acgacctgaa gtgaaaaaag
    17941 agctggcggt attaatgcgt ccgcaggtgc tgtctgcatt gctgacgacg gtactgggag
    18001 ctggtgcaat gtttaccctc tacacctata tctctccggt actgcaaagt attacccacg
    18061 caacaccggt gttcgtcaca gcaatgctgg tgctgattgg tgtcggattc tctatcggta
    18121 actatctcgg cggcaaactg gcagatcgtt cagttaacgg cacgttgaaa ggctttttgt
    18181 tgctgctgat ggtgattatg ctggcaatcc cgttcctggc ccgcaatgag ttcggcgcag
    18241 ctattagcat ggtggtgtgg ggcgctgcaa cctttgcggt cgtaccgccg ttacagatgc
    18301 gcgtgatgcg tgtcgccagt gaagcgccag gtctgtcttc atcagtcaat attggtgcct
    18361 ttaatcttgg aaatgcgctg ggagcagctg ctggtggtgc ggtaatttcc gctgggctgg
    18421 gatacagctt tgtgccggtg atgggggcga ttgtcgcggg actggcatta ttgctggtgt
    18481 ttatgtcagc cagaaaacaa cctgaaacag tttgcgttgc caacagctaa ttaaacaaaa
    18541 acgcagaagg ataaccttct gcgttacagt tgctggcaac gtgcgattta tcttgccgaa
    18601 tgcgctgtta acgcctcatc cgtgttggca tggcgcgcga agcgcatcag gtaatgttgc
    18661 atttgccatc agttattatg cagcgagatt tttcgctacg aattcccagt tcaccagcgc
    18721 ccagaagtgc tccagatagc caggacgtgc attgcgatag tcgatgtaat aagcgtgttc
    18781 ccagacatca acggtcagca gcggagttgc atcggtggtc agtggagtac ccgcgttaga
    18841 ggttgaaacg atagccagtt tgccatcgct gtttttcacc agccaggtcc agccagaacc
    18901 aaagtttttg atcgctgcat cagtaaactg agctttgaaa tcggcaaagc tgccaaaaga
    18961 tgcggcgata gcttcagcga cttttccagt cggttcgcca cccgcgttcg gtgccaggca
    19021 gttccagtag aaagtatggt tccagacctg agctgcgttg ttgaatacgc caccttcaga
    19081 gctgcgaata atctcttcca gtgatttacc ttcaaacgcg gtacctttaa tcaggttgtt
    19141 caggttagtg acataagtct gatgatgctt gccatagtga tactcgatgg tttccgcaga
    19201 aatgtgcggt gccagagcat ctttagcata tggtagtgca ggtaattcga atgacattgc
    19261 tactctcctt tattattaat ttgcatacgt acaatagcct tattgtgcgt atggataggg
    19321 tagcaaatta aaaggtgaga caaaagatga acttaccctg ttgccgtaac aacagggtaa
    19381 agttttagcg aagtgttttt ggggtcatta cccgacgagc gccaacatag tggcgctgcc
    19441 agtagtcttc actgagagaa gtgatttgaa tttcttggcc cgtgcgcggt gactgaataa
    19501 atttgccgtt gccgacatac acgccgacat gatcggctgt accgcgtccc tgagtacgga
    19561 agaagaccag gtcgccgttt ttcagttcac tacgttcgat aggtgctgca tcacgcaggt
    19621 gatacatttc attcgccgta cgcggaatac gaattttcac caaatcttta taagcgtaat
    19681 aaaccaggcc gctgcaatca aaaccggtac gcggtgagct gccgccccaa cgatatggct
    19741 taccaatttg ttgcatcagt ttattcattg ccactttcgt ggctttttgc accttcgctt
    19801 tgtgcgcgtc ggcaagggtt cctgacgcgg catttttgac tttcacacaa tgcgatttat
    19861 agcccttacg tttggtacac ttttcggtaa agttggctga ggccgtttta ttgacggcat
    19921 gtttgctgcg agttttggcg tttttagaag cggtaatgga agatttttta acggaagaag
    19981 cggttttgct tttggtcgta cttttactgg cggctttttt cgctgttttc tggctggctt
    20041 tctgggtctt tttggtggtt gccgtgcttt tctttttatc tgcttttttg gtgatatgag
    20101 tagtagcaga actctccctg gcttgctttg aagcatgggc cataggcgtt aaaggcaggg
    20161 tggtaaaaag taaagcacag agcgtgatcg aaatacggtt tatccgcgcc actgagcgtc
    20221 cctctgttaa ggcaggtcta tcatgcctgc gtctgattta caaaacttga tgattctaat
    20281 gcatagaaga agagaaagtt aatagctttt attgttaaaa aatacgtttc gttggcgagc
    20341 gcaaaaaaat gcagcagtga aagctaaaaa tggttataaa tacgccagtt tgccgtgcgt
    20401 cagggaagaa acgaatattt gaaatgcaac aaatagagca aagggataaa atagcaaaag
    20461 cgtgatgaaa atgttatttt ccgatgccgc actgacccgc tacaatgtca gattactgcc
    20521 gtaaagaagt ttgaggaagc aagacaatga gcaccactat cgaaaaaatc caacgccaga
    20581 ttgctgaaaa cccgatcctg ctgtacatga aaggttcacc gaaactgccg agctgcggtt
    20641 tctctgccca ggcagtccag gcgcttgccg catgtggcga acgttttgcc tatgttgata
    20701 tcctgcagaa tccggacatt cgtgcggaac tgccgaaata tgctaactgg ccgaccttcc
    20761 cgcaactgtg ggttgacggc gagctggtcg gcggttgtga tatcgtgatc gaaatgtatc
    20821 agcgtggcga actgcagcag ctgatcaaag aaactgccgc taaatacaag tctgaagagc
    20881 cggacgctga ataatccgtc ctgcaatact gacggatgcg aacgggcatt cgtcagtcct
    20941 ttctctatcc ccaatccagc ccttgtggcg aacttgaaaa tcccgcctca cgcagcagcg
    21001 taaacatcgg cgtttgccgg accggtaggt cattcacttc tgttagcgta aagcgcagcc
    21061 gtggttcgcg acgcagtgcg gtagtcagcg cgtggaaaat ctctggggcg agtaattcct
    21121 ctttttcctg ccagaccagc atttttttgc caccttgcgc cagatagagt aacaatttgc
    21181 cgccagaaac gaccaccagc gcacccgccc gacgcgttgg tatcagcgtt gccggatgtg
    21241 caggccaggg cagaagattt ccccatgcat tagccggatc gttggcagag agcgccactg
    21301 gtgtatagtg gcgcgtttgc gtggcttgtg tcgccagatc gcgcaatcgg tcaatagtca
    21361 gacgttcagc gaattgcgcg ccacccagac cttctacaaa acgacctcgc ataattcgcc
    21421 cggagtcttc catacttcga caaagcgttt gcatcgatgg aaacccgcca gggatatttt
    21481 cggctatcac cgcctgacga ctgatgatgc cgtagcggtc gagcatattt tccgccagcg
    21541 ccagcatcct ttcggtatcg tttagtggct ccacctgcaa taacgaccag cgtccagcaa
    21601 gctttggtgt gttgtaagat accagcggcg agacgggttg cgcatagaca ggacgtccac
    21661 gacgagcccg gtgactgcgg cgagttgagg tgcgtgtgtt ggtactgctg cgggtgaggg
    21721 cgcgtaacgg tgcccaaatg tcgctggtga tgacgccttg ccagaccagc gcccataacg
    21781 cctcttgcat ggctgaggga tcaaccgatt cgccgatttt atcgcgtatc cgttggctga
    21841 tttgttgtgc aaaccaggct cctccgtcag ccagaaccgt gactatcgct tgctgcagcg
    21901 ccgaactatt cgcctgattc gcttcggcgg gagtgaacga ttctgcggca tattcctgta
    21961 gatgcagtgc caccagaccg tcatcttcac ccagcttttt ttgccccgac cagataaccg
    22021 caccggttgc cagtaattca tcgagcattt ctggcgaata gtcgcgtacg cgggcaggca
    22081 ggatctggct ttcccagagt gaggcgggta aaccgactcc ggcaagctgt tcgatcaccc
    22141 gcatgacgcc atccacgccc tcataaacgc ctggcgatgt tgaggcaaag agtgccgggc
    22201 taccatcggt ggcgggtaat acgccctgac gttccagcag caatcgcgca taggtcgtgg
    22261 ccgcaacggg acgcgtcgct tctctggcgg cttgcagcga gcgcaaacgc agacgacgaa
    22321 atacttcatc gctcacccag atgtcttgtt gcagattcat caccagcccc tgttcgcgca
    22381 gttgctgaag ttgctcttcg acaatggcaa taccgagact aaactcatga gccagttgtt
    22441 cagacgtgac cagagcatga gcccggagat agcgcagaaa gaggtcgcga agcgggtaac
    22501 tgactcggtg caaataaatc tctggcagtg actctggtag tcgtacgccg agagcatcgc
    22561 gcagcctggc ggcatcatcc atacaggcaa gacgctcctg cccgctaatc atggtcggga
    22621 agattcgttt tactgcaaga agattttcca gatacgacgc aatctcttca ctgctgcctg
    22681 tatgttgttg cgccaggtct tcaacggtca ttggccccag ttcgcgcagc aggtcgaaca
    22741 atccttcttc acctttcgct cttctgccag gagccagtcg ttgcaactct tcttccacct
    22801 ggcgaatgac ctgtgggtcg agtaattccc ccggatcgac ctgtccgagt agattgcgca
    22861 gtaactcgct gtccagcgac agtacggatg cccggcgctc tgccagcggg gcgtcgctct
    22921 ggtacataaa ttccgcgaca tagccgaaca ataaactggc ggcgaaaggc gaaggcgtag
    22981 tggtcgttac atcggatatt tgaatttcgc caccgttcag gcgacgcatc aaacgttcca
    23041 gcgcgggaag atcataaaca tcttgcagac attcgcgtag ggtttcgaga atgaccggaa
    23101 aatctggata tccctgagcg atttccagca actgactggc gcgcagccgt tgttgccaga
    23161 gcggggtgcg atggcccggc gtgcggcccg gcattaataa tgtccgcgcg gcgcattcgc
    23221 gaaaacgggc ggcgaaaagt gccgagctgc ctaccgcctc gcggacaatt tgcagcaact
    23281 tttctggttc aaacaaaaaa atcgcggcat caggcaattt accatcggtg tcaggaatgc
    23341 gcgcaacaat gccgtcatca ctggcaacca cagacgcgtc agcgccccat agtgcatgta
    23401 ttcgcccggc aatcgccagc gcccagggtt catgcacccg tcttccatag ggagagtgca
    23461 aaataatacg ccagtcacca atttcatcac ggcaccgttc gaggaccaga tgacgactcc
    23521 ccggaacgat gcccgtcgcg ttgcgctgct cgtcaatcaa cccctgaata ttggcgatcg
    23581 tattttcttc tgccaaccac gggggaatag tcccggaaaa gaacgcgcca tccgccagta
    23641 aatggagaaa atcgccgatc atctcgccta attctgccgg acgtccgtta ccttcaccac
    23701 gccagaaggg aaggcgggca gaacgacctg gagcaggagt cacaatcacc tgatcgcggg
    23761 tgatttgctg gatccgccat gaggtagcgc cgagagtgat aatgtcgttc acccgcgact
    23821 catataccat ctcctcatcc agttcaccca cccgccgcga accggcctgt tcttcacctt
    23881 cgggtaataa cacgctatac atgccacgat ccggaatggt gccaccgctg gtaaccgcca
    23941 acagctgagc gccaggtcgg gcggtcaata tcccggtctc cctgttccag accagttttg
    24001 gtcgaaaagc agaaaaatcg ccagagggat agcgcccgga aagcatatcc agcgtgacgt
    24061 caaaaacacg tcttggcaga tctttccacg gtgcggcacg gcgtacgcgg gagtaccatt
    24121 cgtctgcctg taatgcatcc atcgccgcgg cggcaacggt ttgctgcgca aggacgtcga
    24181 gtggattatg cggcggtgtc aggttttcca gcctgcctgc gaacatacac tctacaatga
    24241 ctgcggaatc gactaagtca cgcctggttc gggggaaaaa aagcccttta gatacaccgc
    24301 caacctgatg tccggcgcga ccaatgcgtt gtaacccact ggcaacagaa agcggcgttg
    24361 ccacctgaat caccagatcc accgcgccca tatcaatccc cagttcgaga ctggaggttg
    24421 cgaccacaca gcgtaattca cccgatttca acgcctgttc ggtgattgct cgttgttctt
    24481 tagagacgga gccgtggtgt gagcgggcaa taaaaatgtc gctactttgt acacggttag
    24541 aggttgcgcc ggaggtcgac tcgaaatggg ccgcatcaac ggcgatagat ggggaacgct
    24601 gtaagcgtgc ggcgtaaagc tcatttaatc gtgccgtcag tttttccgcc agcccgcgtg
    24661 aattagtaaa gacaatggtc gagcgatggc gcaaaacttc atcaaggata cccgtttcaa
    24721 tatatggcca gatggagcct tcccggccgg catggctgtc ttcgccggtg ccgctggcga
    24781 ccgatgagac atcatccata ttggcgaccg gtacgacaat ccgtatctgc ggatggcgca
    24841 ttgcgggcgg gttgactacc gtaaccgggc gatcgccacc aagaaatgct gccacatcgc
    24901 tggctgaacg cacagtggca gaaaggccaa ttcgctgtgc tgaggtgtgg agcagcgtat
    24961 cgagccgctc catacttaac gccagatgcg caccacgttt actgcccgct accgcgtgga
    25021 cttcatcaat aattaccgtt tcgacgccgc gtagcgtttc gcgcgcgcgg gaggtcagca
    25081 tcagatagag tgattcgggt gtggtgatca gaatatccgg cggattacgg gtgagtttgc
    25141 tgcgttcctg tgcaggcgta tcgccagtac ggatccccac gcgaagattg acttccgttt
    25201 cgccgcgccg ccgccgttca tcggcaatac cctgtaacgg gatctgcaag ttgcgctgaa
    25261 cgtcggtgcc cagagctttg atcggtgaaa tatagaggat gcgtgaggtt ttacgcttat
    25321 gcgcctcgcg ggtatcttcg ccgccttcgc ggaagagctg atcgagggcg tagagaaatg
    25381 ctgccagcgt tttcccggag ccagtcggtg caatcaccag cgcatgttcg cctcgcgccg
    25441 ccacatgcca ggtttgcgac tggacagcgg tcggctgttt aaaggcgcga agaaaccagt
    25501 cgcgggtcgc cggtgaaaac acgtccggca ggggcgatga agggtttaga ttatctgcca
    25561 ttaacgctat tccccgtgaa tatcatgcat gatgcgtgga atagcctcac tatagatgcc
    25621 tgaatcattg catgtcatca ggcatcgact cgattacacc tcttcggcgg caggtagcgg
    25681 ccagcctccc agacgtttcc agcggttgac gatttcacaa aacagcacag cagtgcgttc
    25741 ggtgtcgtac agcgcggagt gcgcctgggt gctgtcgaag tccatgccag cggtctggca
    25801 agcctttgac aatacggttt gtccgagtgc cagcccggcc agtgcagcag tgtcaaaagt
    25861 ggcgaaaggg tggaacgggt tacgtttcag tgaggcgcgt tctgcggcgg ccatcataaa
    25921 gctgtgatcg aaattggcgt tgtgcgccac cataatggcg cggttacaac cgctcgcttt
    25981 aataccttta cgcacaactt taaaaatttc gtgcagcgcc tcgtattcgc tgaccgcgcc
    26041 gcgatcggga tcgttcgggt caatgccgtt gaaggcgagg gcctctggct gcaaatttgc
    26101 gccgacaaat ggttcgacgt ggaaatgtaa tgtggtgtcc ggcatcagcc agccttgttc
    26161 atccattttc agggtgatgg cggcaatctc aagcagcgca tcggttttgg cgttaaatcc
    26221 agctgtttca acatcgatca caacaggata aaaaccacga aaacggtcgc acagaccggt
    26281 aagttgagcg ttatcggaca tctgattctc ttaatacgga taaattgcag cgcgcattat
    26341 gacaaatatt acttgtcgat gcagtaaaga tgcgggcgcg atgagttcac gcccggcagg
    26401 agattagttg cccagaccgc gaccggcgtc tttctcttcg attaactcaa ttttgtaacc
    26461 gtccggatct tccacaaacg cgataaccgt agtgccgcct tttaccggac ccgcttcacg
    26521 ggtgacgtta ccgccgttct gacggatttt ttcgcacgct tcagcggcgt tatctacgct
    26581 aagcgcgatg tgaccataag cagtgccgag ttcgtattta tccacgcccc agttgtaggt
    26641 cagttcaatc accgcttctt cggtttccgg gccgtagcca acaaacgcca gtgagtattt
    26701 gtattccggg ttttcgctgg tacgcagcag tttcatgccc agtactttgg tataaaaatc
    26761 gatggagcgt tgcagatcgc caacgcgcag catggtatga agaagacgca tctttatatc
    26821 ctcaaaatgg ctaacagatt caaaatgttg ttttagtata gcggcggctt tgcgccgctc
    26881 gcaatgttgg attacagcgt cgggtaatca gtgtagcctt ccgcgccgcc accgtaaaaa
    26941 ctttcggcac gctgtgggtt aagctcagct ttgcgttgca agcgggcgac cagatccggg
    27001 ttcgcaatcc agtcacgacc aaatgccacc gcatcaatta accctttgcc gatcagcgtt
    27061 tcggcttttt ctaccgtgta tgcacctgcg ccgataatcg gaccgtggaa acgggcgcgt
    27121 actttttcgc ggaacgcatc agtatacggt tcaccccccg cccaatctgg ttctgacata
    27181 tgcagataag caatgccgcg tttacccagt tgttctatca gatacagtgc atcggcttct
    27241 tcattcgggc cgttatctgt gttctggaaa gtaccgattg gcgaaacgcg aatgccaatg
    27301 cgatcggcac cccattcttc aatcccggca tcgaccactt ccagcaccag acgtgcgcga
    27361 ttttccacgc tgccgccgta ctgatcggta cgatggtttg aagaaggaga aaggaactga
    27421 tgcagcaaat aaccatgagc agagtggagc tctaccagat caaaaccggc ttcacgcgcg
    27481 ttagcaatgg cctgacggaa atcattgacg atacccggaa tctcttccag ttcaagcgca
    27541 cgcggcatgg atgtttcaac acggatcgcc tgaccatttt catcgcgcag agaagtacgt
    27601 gttcccgcgc taagtgcgga aggcgctacc ggtgcctgac cgccaggttg caggctggcg
    27661 tgagaaatgc gtccggtgtg ccacagctgt acggccatat gaccattttc agcatgaacg
    27721 ccagcggtaa tttttttcca tgcggcaatt tgttccggac tatggatgcc aggtgcacca
    27781 gcataccctt ttgcctgggc agaaatttgc gtggcttcgc taataatcaa accggcactg
    27841 gcacgttggc gatagtattc cgccatcaac ggggtaggga tgtcacccgg ttcaatactg
    27901 cgcagacgcg tcagcggtgc cataaaaata cggtttgccg ccgtgatcgc gcccactttc
    27961 agtggggaat acagtttttc agatgacata aaggctcccg gtcgtctggt gataaaaaat
    28021 gcctgctaaa cggcaggcgt cgcaataatg gtttttacat gtgccagcgc gttttccagt
    28081 ggctcgaaac tgcgcgaaat tttggcctgc agattcgcac ccagccatag tgcgtaaagc
    28141 acttgtgcct gttgcagcgg ttcgccacaa aaggttaaac aatggctatc acggccattt
    28201 tccagcgcct gcgagagcag ggcgatcacg ccgcgagcgc ctttatccat cgcgctacgc
    28261 atatcttctg acagatcgca cacttcggca gagagtttta ctgtcaggca accactgatg
    28321 gttccatgtt ggcaaaactg gttcagtgtt tgctggtaat aagccagtat gcggtcgcgg
    28381 tagttacctt cgccggattg cagcaactca gtcagtcgtt ggtgatatgt ggcgtaatgg
    28441 cgctcaagca tggcaacgcc aaacgcttct ttagagcgaa agtagtgata gaaggaccct
    28501 ttcggcactt cagcggtttt tagtaattcg cttagcccca tcccggtgaa tccacgttgc
    28561 aggcaaagtt gctcgcccgt cgccaggaga tgttcgcgag tatcatgttc ggtgtgtttg
    28621 ttcatgcgtt ggagtgtagt agaccagtcg gtctagtgca agcaaagttg ctactttggg
    28681 cattaacagc ttcaataatg gtaataatct tatttgagga gggcgaagtg gcggagcaat
    28741 tagagttctt tcccgttcag agcccgtgcc ggggaatttg ccagtctgac gaacgcgggt
    28801 tttgtcgcgg ctgttttcgt agtcgggatg agcgttttaa ctggaataaa atgagcgatg
    28861 gcgaaaaaca agaggtgctg cgcctttgtc ggcagcgatt aatgcgcaaa ttacgcgcaa
    28921 ataagccagc gtcatcagac gaaccggagc aaccatcact cttttgagcc taatcttgcg
    28981 tatactcctc tcatatctga taagaggaag cgattatggt tcagcgtatt actattgcgc
    29041 cgcaaggccc ggagttttcc cgttttgtga tgggctactg gcgattgatg gactggaata
    29101 tgtccgcccg ccagctggtc agttttattg aagagcatct ggatctcggc gtgaccaccg
    29161 tggaccatgc tgatatttat ggtggctatc agtgcgaagc ggcgtttggc gaggcgctga
    29221 aactggctcc tcaccttcgt gaacggatgg aaatcgtcag taaatgcggt atcgcgacga
    29281 ccgcgcgtga agaaaacgtc attggtcatt acattactga ccgcgatcac atcattaaga
    29341 gcgccgaaca gtcgctaatt aatcttgcga ccgattatct ggatctgctg ttaatccacc
    29401 gaccagaccc gttaatggat gccgatgaag tggcggacgc gtttaaacat ctgcatcaga
    29461 gcggcaaagt gcgccatttt ggcgtatcga actttacgcc tgcgcaattt gccctgttgc
    29521 aatcacgtct gccgtttacc cttgccacta atcaggtgga aatatccccg gtgcatcagc
    29581 cgttactgct ggatggcacg ctcgaccaac tgcaacaact gcgtgttcgt ccgatggcgt
    29641 ggtcctgcct tggtggtggt cgtctgttta atgatgatta tttccagccg ctgcgtgatg
    29701 aactggctgt ggtggcggag gagttaaacg cgggctcgat tgaacaggtg gtttacgcct
    29761 gggtattacg tttaccgtcg cagccgctgc caattatcgg ctcaggtaaa attgagcgtg
    29821 tacgggcagc tgtcgaagct gaaacactga aaatgacccg tcaacaatgg ttccgtatcc
    29881 gtaaagcggc attgggttac gacgtaccgt aagcactttt aggaatagcc gccgttcaaa
    29941 aatgtgtcac tggtttacac ttattcagga atgcacaatg aacggaggtc ctatgaaacg
    30001 ttttagtctg gccattctgg cgctggttgt tgcgaccggc gcacaagctg ccagtgaaaa
    30061 agtcgagatg aacctcgtca cgtcgcaagg ggttgggcag tcaattggta gcgtcaccat
    30121 tactgaaacc gataaaggtc tggagttttc gcccgatctg aaagcattac cccccggtga
    30181 acatggcttc catattcatg ccaaaggaag ctgccagcca gccaccaaag atggcaaagc
    30241 cagcgccgcg gaatccgcag gcgggcatct tgatccacaa aataccggta aacatgaagg
    30301 gccagaaggt gccgggcatt taggcgatct gcctgcactg gtcgtcaata atgacggcaa
    30361 agctaccgat gccgtcatcg cacctcgtct gaaatcactg gatgaagtca aagacaaagc
    30421 actgatggtc cacgttggcg gcgataatat gtccgatcaa cctaaaccgc tgggcggtgg
    30481 cggtgaacgc tatgcctgtg gtgtaattaa gtaattaaga ggccagcgta ccttgtggcg
    30541 gtgcttgctc aagttgcgaa agcgagcagt acagccgcca gacaattgcc gccagctcgc
    30601 gggcggcagg ttgatgatgg cgggcaaggc tgtcgcaaat ccgctgtaat tcttcaagtg
    30661 tggccgccag tgatttttgc tgaacgccac gctcactcat cactccccgc aacagtgaaa
    30721 tacagttatc ccgtactcgc gataacggat cggaacgcga ttcccagtcg cgcaattgcc
    30781 agacaacatg agaacagttc agcagcacta caccccagcg taataaccaa cggcgagcca
    30841 gcgcatcctg gctgttactc aactgactga cgtgatgata agtgagcgat tcaaattcgc
    30901 tttcactcag tgttggatgg cggcttagtt gatcgacaaa atcccggcgc agcgcgcgta
    30961 tatggcggcg gcttttgcga gcatccgatc ctggacgcag aatggcgaac gctaaccacg
    31021 ccaacgcgac gccaacgatt tttgccagat tatcgttaag aaaatcagca aaatcataca
    31081 ccgggggatt agtgacagcg ataaacgaac ccataaaaac aatcagttgc ccccacagtg
    31141 cggcaaattt gggcatctgc aatttaagaa gctgcattgt cgccagcagt ggaaagagaa
    31201 acagtaaaaa ttgccacaga tcgctaatct gcaccatcag gccaaatttg accacaaagc
    31261 tgaatagcga aagtaacacc agcgtgcgca tcagcagtga caacgactta aacggtgctg
    31321 cgacggcgga gtagagtacg cagctaattg ctgccagcgt taatgcattg gaaccggcat
    31381 cccattgcga agcaatactc catgcgccaa tcatcatcaa cgtacaaaat gtacgcagtc
    31441 cgctccacat agcttcggcg ttatcggtat gacgagccag gccggatgtc cgtgggagtc
    31501 tggtatgatc atccacaccg ctttgcagat gttgtaattc ctgactactt tgcagataaa
    31561 ggcggcaaaa ataacgtagt cgctgccaga aggcgacgtg ccgatagtcg gcgacgttgg
    31621 tcgggcgtag cggggcgata atacgtgcga cggtgtaaac atctgtttgc gaactggcga
    31681 gcgccgtcag cagctgttcg agaatttctc gtgtggcaca tggcggtgag ggccagttga
    31741 gcaacatacg tcgcaggctg gagatgacac tggtcatacg taattgctgg tggagcagcg
    31801 cattaaggcg tgcgttttgc tggcgaaaac gatagtggct ccagaaagcc tggatacgga
    31861 gcaaattcat ggtcagtatc tgtccaatca ccccctcatg tgctgcacga atggcatcgg
    31921 ttgtttcagg ctgccagagt aaactggcat gttccagtaa tcgggcgtgc atgtttttca
    31981 atgcggttaa aagggctgta gcatcggaac tgctcggcag gatcatcatc atcatgccgc
    32041 cgcacaaaat accgacaatc acctcgcaaa cgcgcgcctg agcgatatcc cacagctggc
    32101 tggcctcagt aatattaacc atcggaaagg cgatgatggc agctgtgtag cccgccagtt
    32161 gaaatgcata cgcgacatta ttcgtgaagt gcgcacaggc ccaggtacaa aagccaagcc
    32221 acgccgccat gctcaaaaga aaaaaccacg gctcattgag cgtatgtccg gcaagaagca
    32281 gtgccgcaat ggctccgagc aaactgccag cgatgcgtcc gaggcttttg ctgatgacac
    32341 cgccaacggt gggaaagcta accacggcag ccgaggtcat cgcccaatag ggttcatcca
    32401 gatttaaata ataggcaacc gtcagcgcca gacacatggc aatggtattg cgtaacgcat
    32461 aacgccattg cgccagcgtg gccctgaacc agggcaaatt gcgcaaggac catgacgatg
    32521 cgttcattat tgacctacgg caatactaca ggtggtgccc gacaccagcg tgacatcgcc
    32581 tgggacttta tccagcgcaa agcggacggg aactcgttgg gcgaggcgaa cccaggggac
    32641 gttaggtttc acatccggaa taaggtttga gtcactttca acgctctgat cataaatcgc
    32701 ccgaccgata ctggaaacgt gaccctgtaa cgttttgtta tcactataga gggtaatttg
    32761 tgctggtgca ccttcgcgga tatggcgcaa cttggtttct tcaaaataac caatgacgta
    32821 aaacgagtgg ctgtcgacaa gggcaaacag cggtttcccg gtgtcagcgt aatcgccgat
    32881 gcgggtagtg aggttagtca cccatccact caccggggcg cgaatttccg tttgcgccag
    32941 ttgccattgt gcctgtttca gcgtcgcctg tgcggcatca acactggcct gcatcgcttt
    33001 aacattgagg ttagcggtat cgagttcttc ggcagaaata aaattttgtg agagatgacg
    33061 acgacgattc gcttcgttat tggcttttgc gaggtcagat tgcgctttcg ccaactgagc
    33121 ctgtgcgttc agctcggcga tctgaaaggg cgttttatcg atcgtgagca gaagatcccc
    33181 cgcgttaacc agttggttgt cttttatatt cagctcaaca atacgtcccg acacctgagg
    33241 ggtgatagaa acctgttctg cgcgtatttt cccgtcgcgt gtccacggag attgcatgta
    33301 ataattccac agccaccatc cggcaagaac cgcaactacc gctacaatga ttgttgagaa
    33361 atacttaatt gttttaattg acatagttac cacgcaatca gtattgccag ggaaaggcaa
    33421 acgcaaatcg caaacagcga taaatccatt aacaagggat gccagatgtc accggcgtag
    33481 atccagccac gaagcaggcg gtgtacgaca agccagatga cgaatccaaa cgcgaaggct
    33541 ttgaaaaacg gaggaaagta gacggacgca ccgaacacca gatcttgtaa gggcaatcct
    33601 gttgcattga gcataaactt cacaaagtag attcctttac gaccgaatat attgcgttag
    33661 attaataaat attctttaag tgcgaaaaat ttacgcgcaa tttctgaaaa atatgtctga
    33721 cggtaaccaa atgcagcaat acatttgttt tagcaataca attgctgcac actattctaa
    33781 aagccgcata atatcttagc aagctaatta taaggagatg aaattggaat cgccactagg
    33841 ttctgatctg gcacggttgg tgcgcatatg gcgtgctctg atagaccatc gcctgaaacc
    33901 gctggagtta acacaaaccc attgggttac gttacacaat atccatcagt tacctccaga
    33961 ccagtcgcaa attcaactgg caaaagcgat tggcatcgag cagccatcac tggtccgtac
    34021 tctggaccaa ctggaagaaa aagggttaat ttcgcgtcaa acttgtgcca gcgatcgtcg
    34081 ggctaaacgt attaaactga cggaaaaggc agagccgctg atcagcgaaa tggaagctgt
    34141 tattaacaaa acccgcgcgg aaattttaca tggcatctcc gcagaggaac tggaacaact
    34201 gattaagctc atcgcaaaac ttgagcataa tatcattgag ttacaggcca aagggtgaaa
    34261 tgaagggggc gtgtggtcag taacctgacc acacgcaaac ttatttagcg cggggaaacg
    34321 gtcacctgac tgccattgct ggccagtacg acacgttggc ccggagagaa acgagtgttg
    34381 ccttgtttct gtaccaccat gatggtatta ccatcgtctt tacgaatttc cagctcgaca
    34441 ccctgcgttt tgttcattgc actctgtacg ccctgaccgg ctacgccacc tgcaacagcg
    34501 cctgcagcag tagccagaga acgcccggtt ccgccaccaa cagtattccc caggaaacca
    34561 ccaagaacag caccgccaat tgcgccgata acgttggaat catcaccgcc ctgaatctgt
    34621 accggacgta cgttaacgat ggtgccatag ctgacattct gtacttgttt cgcttcagaa
    34681 gcggtataaa catcccctga cagggtgtcg ttattaacac aaccgacaag agacagacct
    34741 accattgaaa caaccaatac gcgtttaatc attgaaaaat ctcctgttca ccatgaaacg
    34801 ccacgcaagc atccctcatg gtcaaagtat atggcatatg tgaatccatg gttacatgtt
    34861 ctaccaaata tgagcaaatc ataatgaaag taggatcaac caattataaa caaaacattc
    34921 atgatgatta ttgatttgaa gataatgttt tactaccaac ggtaaaaatc ggtcattccg
    34981 catggtattc acgaaaagtt tatgtttgag tggtgcgcaa acgtcagatt gaaaaaggat
    35041 gaactatgaa atcgggccgc tttattggcg ttatgtcagg caccagcctt gatggtgttg
    35101 atgttgtgtt ggcgacaatt gatgaacacc gggtcgcaca gctggcaagt ttgagctggc
    35161 cgatcccggt gtctctgaaa caggctgtac tggatatttg ccagggccag cagcttatac
    35221 tttctcagtt tggacagctt gatactcaac tcgggcgact ttttgctgat gcggtcaatg
    35281 ccttgcttaa ggaacaaaac ctgcaggcga gagatatcgt tgcgatcggc tgtcacggtc
    35341 aaaccgtctg gcatgaaccg acgggcgtgg caccacacac tttacagatt ggcgataaca
    35401 atcaaattgt ggcacgcacc ggaattacgg ttgtcggtga ttttcgccgt cgcgatattg
    35461 ccttgggagg acaaggcgca ccgctggtac ctgcgttcca tcatgcgctg ctggctcacc
    35521 caaccgagcg acgaatggtg ctcaatattg gcggcatcgc caatctgtca ctgctcattc
    35581 ctgggcagcc ggttgggggc tacgataccg gtcctggtaa catgctgatg gatgcctgga
    35641 tctggcgtca ggccggtaaa ccttacgata aagatgccga gtgggcacgg gcgggtaaag
    35701 ttattctccc actgctgcaa aatatgctca gcgacccgta tttctcgcaa cccgcaccga
    35761 aaagcaccgg acgcgaatac tttaactacg gctggctgga gcgccatttg cgccattttc
    35821 cgggtgttga tccccgtgat gtgcaggcca cactggcaga actcaccgcc gtgaccattt
    35881 ctgaacaagt tttgttgagc ggtggctgtg aacgattgat ggtatgtggt ggaggaagtc
    35941 gtaatccgct actcatggcg cgtctggcgg cattactgcc aggcacagaa gtcaccacca
    36001 ctgatgccgt tggcattagt ggcgatgaca tggaagcatt ggctttcgcc tggcttgcct
    36061 ggcggacgct tgcgggatta ccaggaaatc tgccttccgt cactggcgca agccaggaga
    36121 cggtactcgg ggctattttc cccgctaacc cgtgatatca gagttaactg aatttttctt
    36181 accgcgtctg ccgttacact tggaacgtta gggagggcgt attgccctcc agaccaggaa
    36241 agtcttcggg atatgtctat gaaaaaactg ttactgatct gtttgccggt ccttctcacc
    36301 ggctgtagcg cgttcaatca actgtttgag cgcatgcaaa ccgatacgct ggaataccag
    36361 tgtgatgaaa aaccgttgac ggtcaaactg aataatccgc gccaggaagt cagttttgtt
    36421 tacgataatc aactactgca tctcaaacag ggcatttctg cgtctggtgc acgttacacc
    36481 gacgggatct atgttttctg gtcgaaaggc gaggaagcga ctgtctataa acgcgaccgc
    36541 atcgtcttga ataactgtca gttacaaaat ccacagcgtt gagatttttc caggggcggc
    36601 gcacaatagc gtcacccact gacaatccgt aaagaaaacc atgtctgata acgacgaatt
    36661 gcagcaaatc gcgcatctgc gccgtgaata caccaaaggc ggattacgcc gccgcgatct
    36721 tcccgccgat ccattaaccc tttttgaacg ctggctctct caggcgtgtg aagccaaact
    36781 ggcggaccct accgcgatgg tggtcgctac cgtggatgaa catggtcagc cttatcagcg
    36841 catcgtttta ctcaaacatt acgacgaaaa aggcatggtg ttttacacca acctcggcag
    36901 ccgtaaagca catcaaatcg aaaataatcc gcgcgttagc ctgctgttcc cgtggcatac
    36961 ccttgagcgc caggtgatgg tgatcggtaa agcggaacga ctttcgactc tcgaagtgat
    37021 gaaatatttt catagtcgcc cacgtgatag ccagattggt gcatgggttt cgaagcagtc
    37081 cagtcgcatt tctgcccgcg gtatccttga aagtaaattc ctggagctga agcagaagtt
    37141 tcaacagggc gaagtgccat tgccgagttt ttggggcggt tttcgcgtca gccttgaaca
    37201 gattgagttc tggcagggcg gtgagcatcg cctgcatgac cgctttttgt accagcgtga
    37261 aaatgatgcg tggaagattg atcgtcttgc accctgaaaa gatgcaaaaa tcttgcttta
    37321 atcgctggta ctcctgattc tggcacttta ttctatgtct atttcgcatc tggcgaaaag
    37381 tcgtgtaccg gcaaaggtgc agtcgttata tacatggaga ttttgatggc aagcagtaac
    37441 ttgattaaac aattgcaaga gcgggggctg gtagcccagg tgacggacga ggaagcgtta
    37501 gcagagcgac tggcgcaagg cccgatcgcg ctctattgcg gcttcgatcc taccgctgac
    37561 agcttgcatt tggggcatct tgttccattg ttatgcctga aacgcttcca gcaggcgggc
    37621 cacaagccgg ttgcgctggt aggcggcgcg acgggtctga ttggcgaccc gagcttcaaa
    37681 gctgctgagc gtaagctgaa caccgaagaa actgttcagg aatgggtgga caaaatccgc
    37741 aagcaggttg ccccgttcct cgatttcgac tgtggagaaa actctgctat cgcggcgaac
    37801 aactatgact ggttcggcaa tatgaatgtg ctgaccttcc tgcgcgatat tggcaaacac
    37861 ttctccgtta accagatgat caacaaagaa gcggttaagc agcgtctcaa ccgtgaagat
    37921 caggggattt cgttcactga gttttcctac aacctgttgc agggttatga cttcgcctgt
    37981 ctgaacaaac agtacggtgt ggtgctgcaa attggtggtt ctgaccagtg gggtaacatc
    38041 acttctggta tcgacctgac ccgtcgtctg catcagaatc aggtgtttgg cctgaccgtt
    38101 ccgctgatca ccaaagcaga cggcaccaaa tttggtaaaa ctgaaggcgg cgcagtctgg
    38161 ttggatccga agaaaaccag cccgtacaaa ttctaccagt tctggatcaa cacagcggat
    38221 gccgacgttt accgcttcct gaagttcttc accttcatga gcattgaaga gatcaacgcc
    38281 ctggaagaag aagataaaaa cagcggtaaa gcaccgcgcg cccagtatgt gcttgcagaa
    38341 caggtgacac gtctggtgca tggtgaagaa ggtttgcagg cggcaaaacg tattaccgaa
    38401 tgcctgttca gcggttcctt gagtgcgctg agtgaagcgg acttcgaaca gctggcgcag
    38461 gacggcgtac cgatggttga gatggagaag ggcgcagacc tgatgcaggc gctggtcgat
    38521 tctgaactgc aaccttcccg tggtcaggcg cgtaaaacta tcgcctccaa tgccatcacc
    38581 attaacggtg aaaaacagtc cgatcctgaa tacttcttta aagaagaaga tcgtctgttt
    38641 ggtcgtttta ccttattgcg tcgcggcaaa aagaattact gtctgatttg ctggaaataa
    38701 tgcattaagt ggaaaggggg agtgagaaat cactccccct ggtttttata cagggaacat
    38761 gatgaaaaat attctcgcta tccagtctca cgttgtttat ggtcatgcgg gtaacagtgc
    38821 ggcagagttt ccgatgcgcc gcctgggcgc gaacgtctgg ccgctgaaca ccgttcaatt
    38881 ttctaatcac actcaatacg gcaaatggac tggctgcgtg atgccgccca gccatttaac
    38941 cgaaattgtg caaggcattg ccgccattga taaattacac acctgtgatg ccgtattaag
    39001 tggctatctg ggttcggcgg agcagggtga acatattctc ggtatcgttc gtcaggtgaa
    39061 agcagcgaat ccgcaggcga aatatttttg cgatccggta atgggtcatc cggaaaaagg
    39121 ctgtatcgtt gcaccgggtg tcgcagagtt tcatgtgcgg cacggtttgc ctgccagcga
    39181 tatcattgcg ccgaatctgg ttgagctgga aatactctgt gagcatgcgg taaataacgt
    39241 cgaagaagcg gttctggcag cgcgtgaact cattgcgcaa gggccacaaa ttgtgttggt
    39301 taaacacctg gcgcgggctg gctacagccg tgaccgtttt gaaatgctgc tggttaccgc
    39361 cgatgaagcc tggcatatca gccgtccgct ggtggatttt ggtatgcgcc agccggtagg
    39421 tgttggtgat gtgacgagcg gtttactgct ggtgaaactg cttcaggggg caacgctgca
    39481 ggaggctctg gaacatgtga ccgctgcagt atacgaaatc atggtgacca ccaaagcaat
    39541 gcaggaatat gagctgcaag tggttgctgc tcaggatcgt attgccaaac cagaacatta
    39601 tttcagcgca acaaagctct gaaatatctc atttcggccc acaacgacct gtgggccgat
    39661 ggctattact ttaagccttc cgctgacagc gcgtcttgta cttccggacg ttcagccata
    39721 cgttgcataa atgccgcaat gtgctctaac ccttccagat tcagtttcac cgcgtatgcc
    39781 cagcgcagaa ccgtaaacag ataggcatca gcaattgtaa atcgttgccc gcagatccag
    39841 tgctcatcct tcagtgcctc gttcacatat tgcagcttct tctccagctg cgcgcgaact
    39901 gtcgatttgt actcttccgg tgtatcaggg cgaaacagag gtgtgaaacc tttatgcagc
    39961 tcggtggcga tgtaattcag ccattcgatg gttttatagc gggaaatact gtttaccggt
    40021 gccagcaact ggcggtcggg gacgctgtcg gcaagatact gcataatcgc tacgccttcc
    40081 gtcagcaaag taccatcgtc cagcagcaat gcaggtacct gtcccttagg gttaacggca
    40141 aagtaatcgt caccgttttc gagacgtttt ttcattaaat ccacactgac gagcgtaaaa
    40201 tcctttccgc tctcacgcag ggtgatatgg gaagcgagag agcaggcacc cggtttgtag
    40261 aacaatttca tcggtaactc cttttgtctg tggtttaaag tatggtagtg cgttgctgaa
    40321 caaaaaaaaa gccgctaacg aaaagttagc ggcttgtaaa agagtttccc tgaattttac
    40381 gctacggctg ctttcgccgc tttgtctgca gcgtcatcct gcgtcatgcg gtgcagtttc
    40441 ggcgcggtca gcagcatcag tactgcaata acggcagtag cgacaccaat ctgcaagaat
    40501 acgcgaccat agacttccag tgacatcagt ggatcggtaa cgttatccgg cacagccatc
    40561 atacccgcaa cataaccacc aatcaggttt gcaccggcag tggtcaggaa ccagctaccc
    40621 ataatgaagc ccatcagacg ctgcggaacg agttgtgcaa ccattgccag acccagacca
    40681 gagatcatca gttctccgat actctgcagg ccatagcttg cgaccagcca gcttacagac
    40741 acgatacctg cgtcagatgc gaattttgca cccagcggca gaatcaggaa cgcaccagaa
    40801 cacatcacca taccgattgc aaacttagtt ggcatcggca gggtatcgcc catcttgtta
    40861 tagatagcgg ccagaatcgg gctaccaatg atgatccaga acgggttcag tgcctgatac
    40921 tgctcaggtt ctacggccag gcccagaatg gtgtgttcaa cgttacgaat cgcaaagaag
    40981 ttcagtgacg ttggcatctg gctgtacagc acgaagaaga taatggcttc gagcatcagg
    41041 atgaaggcaa cgatcatttt acgacgcgca gcacctttca tcgcgaaggc ttctttaccg
    41101 aagataacca cgataccaaa ggcaacaacg cccagcgcca tacgcgcaac ttcctgattg
    41161 tgcagcagcc aggtggcgat agcgatcagt gccacaacac caataatggt cagcagcagg
    41221 ttacggtagt tgataggctc gaagtctggt tttgaaccgt actgtttaac ccagcgttgg
    41281 cagaaggcga agttaacgat agtgatcagc aggcctacaa cgctcaacgc aaacgcaaca
    41341 ctccagccgt atttcgcggc cagccacggg gtggcgatca tagagaagaa agagccgatg
    41401 ttgacggaca tgtagtacat ggtgaatgca ccgtccagac gcgggtcgtt tttctcatag
    41461 catgtagaaa gcagagaaga cgggttagct ttaaacaggc cgttaccgac cgcaatagcc
    41521 gccataccca tataaacgat accggcgtcg tgaccagacc aggcaaccag agcataacca
    41581 atcgccagca caatagcgcc aagcataatt acgcgtttag tacccagtac cttgtcacct
    41641 aaccagccgc cgatagcgac cagaccataa accagggcac taaaggaaga gaaaagggtg
    41701 attgaatccg cttcagacat acccagttgt ttaaccaggt aaacagccat aattccttgt
    41761 aggccgtaat aaccaaaacg ttcccataac tcaatcgaga agatgagata gaacgccttc
    41821 ggttgtttga aagcgttcaa actgacgctt tcagttggtt tttggtttgc agtggacact
    41881 tttacctctt tttttacatc ccatattaac gggggtgctt tctcgcgtta tgcctaaaaa
    41941 acatacgcgc aattgttata gtgggagggg aaacggcagg taatgttcac tatcttgcac
    42001 tttcagacaa gtcctttgta ataatctgtt acatataact gaggcgggga attcaaccat
    42061 tccgggaaat gttatccagc gttaaatttt tcaaaaaacg taacggcaga ttttttcact
    42121 accctgagag ggggatgatg gcttaacggc gatatgttct gctatttagc gtttttccta
    42181 gtgagatagt ccagtttctg aaaaatagcc agtgtaatgt tttgtaggtc aatatttgtt
    42241 gcgtgtgtaa atttaaatga ttttaaccta gttcttgcaa tgtttttaca ttgccacagc
    42301 atcgtgatct tgatcacgaa tgaatagaaa atttcgctat taaaaaaaca attaacctgc
    42361 atttttggtt atccgcaaac ggattacctg atgataacgc taagcaatgg catcattgac
    42421 gttaaaaaga tgttttaaaa gaggataaag aaaggttatc aatggggtaa tcggtgttac
    42481 cccttttctt cagatgtcaa ctttctcttt gtattcacaa agatcttcaa taatacaaga
    42541 gccacagcga ggcttgcggg caatacaggt ataacgcccg tgcaggatca accaatggtg
    42601 gcagtcgact ttaaactctg ccggaaccac tttcagtagc ttttcttcta cctgttcgac
    42661 gtttttcccc ggcgcaaatt gagtacgatt acaaacgcgg aaaatgtgcg tgtcgacagc
    42721 aatagtaggc cagccgaatg cagtgtttaa tacgacgttg gctgttttac gacctacacc
    42781 aggcagggct tcaagcgcag cacgatcttc cggaacctcg ccattatgct gttccagtaa
    42841 gatacggcag gttttgatga tattttctgc tttgctgtta taaagcccaa tggttttgat
    42901 ataggttttc accccttcga cgcccagttc aagcatcgct gcaggtgtat tagccaccgg
    42961 gtagagtttc gccgtcgcct tattaacact gacatcggtc gcctgagcgg aaagcagtac
    43021 ggcaatcagc aattcaaaag gcgaactgaa attaagctcg gtggtgggat gaggattgtt
    43081 ctcacgcagg cgagtgagga tctccaggcg ttttgctttg ttcatcagac attccctgtt
    43141 tcaccgtttg gcaatgcacg ttcggcagct gcttcagcac ggcgcttttt cattctttca
    43201 tcaatcaggt atttccctgc cagcatcagt cccaggccaa taaatgcacc tggtggcagc
    43261 atcgccagca ggaaagggga gtcggtgtgg aatatctcca cgcgtaatac ttttgcccag
    43321 ctacctaaca gcgcatctgc accgtcaaac aatgtgccat tgccgataat ttcgcgtagt
    43381 gaacccagca cgaacatggc gcaggttgcg cccataccaa ttgaaaagcc gtccagtgcc
    43441 gaaagcgccg gacctttttt ggcggcgaag gcttcagcgc ggcccacaac gatacagtta
    43501 gtgacaatca gcggaataaa aatccctaat gattgataca ggccaaaggc gtaggcgttg
    43561 atcagcatct gtacagcgct gaccaccgag gcgatgatca tcacgtaaat gggaatgcgg
    43621 atctcggctg gcgtccagtg acgcagcgtc gaaatggtca agttggtcag cgtcagtacc
    43681 agcgtagtcg caagtcctaa acccagagcg ttagtggcag tggacgtgac cgccaatagc
    43741 ggacagaggc cgagcaactg gaccagcgca gagttgtttt tccacaaccc ctgaacaata
    43801 acgtctttaa tttcgctcat gctttattct ccacaggcag gaagttgaga aagttgtgcc
    43861 ggtaacgtct gagcgtacaa tccggcgcgt tttaccgcat ttaccaccgc gcggggagta
    43921 atcgtcgcgc cggtgaactg gtcgaaatca ccaccatctt tcttcaccgc ccagtttgca
    43981 tcatccgcac cactgatttt tttacccgca aaatgggtga tccagtcaga aaggcgcagt
    44041 tcgattttat cgccaagccc tggcgtttcg tggtgctctg tcacgcgcgt gccaagtacc
    44101 gtgccgttaa aatcggctcc caccagcagc tgaatcgcac cggaatagcc atctggcgcg
    44161 gttgtttcca gaacggcggc taccggtttg tcatcctgtt tggcgatgta aacccgatgc
    44221 tcacctttac ctaactctgg cgcagtgacc agatagcaac tctgtgccag cgcattgtta
    44281 tagcgttcgg ctggcagcac ctgatcaaat aacgcctttt gttgcagact ggcctgttca
    44341 gcaattgtcg ttttggtcat ctggttgatg gccgcagtta accctgtaga acccgctgca
    44401 aatagtgcca gtgtaatgcc gtgttttcgg atagttttca gcatggttta ccctttgcga
    44461 tggccgtaga cgcgcggacg cgtgtagtaa tcgatcagag gaaccgtgat gttcgccagc
    44521 aggacggcaa aagccacgcc gtcaggatag ccgccgaaac tgcggatcaa ccagactaat
    44581 aaacccgcca gcgcgccgaa catcagacga ccacgattgg tcgtagaagc agtaaccggg
    44641 tcagtcaaaa taaagaatgc gccgagcata gtcgcaccag acagcagatg aatttgcggt
    44701 gatgccagtg tttctggtga gaacaaccag cccaacgttg cgcataacgc cagcgttact
    44761 aagaagctga ggggaatatg ccagcgaatc gctttctgcc atagcaacca cacgccgcca
    44821 gccagccagg cgagatttac ccattgccaa ccaacgcccg ccagcatacc gctgtagatc
    44881 ggatattgca taatctgttc aaccgaatga ccggcacgga cagaggtttt aaatgtatcc
    44941 aacggtgtcg cctgactaat gccatcaata cctaagcgta gcgtgttcat atcaccacca
    45001 ctggtggtat gtccgctgaa aataacctgt atggcgtcga taaaaccagg gatgttgacc
    45061 gcaatttcat gtggcggtaa ccagctggtc atctgcaccg ggaaggagat cagtaagacc
    45121 acataaccaa tcattgccgg attaaacggg ttttgcccca gaccgccata caactgttta
    45181 gcaatgatca ccgcaaacac cgtacccagc acgaccatcc accatggcgc gaggggggga
    45241 atacttaccg ccagcaataa gcctgtcagc aatgctgagt tatctttcaa cgttgcggct
    45301 accgactgct tgcgtagttt gagtacgaga gcttcggcta acagagcact gaccgacgcc
    45361 aacaggatct gaacgagagt accccaacca aaaaaccaca gttgcgctgc gattcctggc
    45421 acggctgcga gcaacaccaa cagcataatg cgcgatgttt ggcgctggtt atgggtataa
    45481 ggggagctag ctattctgaa taccatttag tcctcgttta caaccttttg ctgggcggct
    45541 tttttggcct gaacgcgggc aatagccgca gcaactgccg ctttgcgcgg atcaatctgt
    45601 tcttctggct ccgcattagc ctgttgctgt tccagcttgc gtgctttggc acgggcaata
    45661 gcagcttcga cggcggcttt gcgcggatca acctgttctt ctggttctgc gttagcctgt
    45721 tgctgttcca gcttgcgcgc tttggcacgg gcaatagccg cttcgacggc agctttgcgc
    45781 ggatcgacct gttcttctgg ttccgcatta gcctgttgct gttccagctt gcatgctttg
    45841 gcacgggcaa tagcggcttc gacggcggct ttgcgcggat cgacctgttg ttctggctcg
    45901 gcattagcct gttgctgctc cagcttgcgc gctttggcac gggcaatagc ggcttcgacg
    45961 gcggctttgc gcggatcgac ctgttcttct ggttccgcat tagcctgttg ctgttccagc
    46021 ttgcgcgctt tggcgcgggc gatagcggct tcaacggcag ttttacgtgg atcagcaacg
    46081 gttgctgcgt cgttagtttg ctgcagttct gcctgtttcg ctctggcttg cgctttacgg
    46141 gcttcccgtg ctgcaataat tgcactgtta tccgggcgtt cgcccgcttt aatcacaata
    46201 ggctgtgtag cctgggcctg tttctctttc acccgcgcca gagcggcagc aatcgcatct
    46261 ttatctttgg ctgcaggttg aacggctgcg ctcttatgtc gttcaaggcg agccgctttt
    46321 tcgcgctcca gacgagcctg gcgcgcttcg aaacgcgctt tggcttctgc ggcgcgcttt
    46381 tcttcctgac gaatagctgc aatttcagct ttttcctgac ggaaatattg caccagggga
    46441 atattgctcg ggcaaaccca cgcgcaagcc ccacattcaa tgcagtcggc aatgttatgc
    46501 gtggtagctt tatcgtgttg ctgacctttg ctgaaccagt acaactgttg cggcaaaaga
    46561 tccgcagggc aggcgtcagc acaggcgcta caccggatgc agctttgttc ttcctgtggt
    46621 tcgccaagct cattggcaga gggagccaac agacagttgg taatctttac gaccgggaca
    46681 tccagccatg gcaaggtaaa gcccattagc gggccgccca taatcaccat ttgatcggca
    46741 gaggggcaga atccggcgtc attcaataaa tgacgcactg gcgtccccag ccgtgcccag
    46801 acgttgcccg ggcgagcgat tgcttcgcca gtcagggtta cgacacgctc ggtaatcggc
    46861 tcgccatcaa taacggcacg tttcactgca taagcagtgc cgacgttttg cattaatacg
    46921 ccgatatcgg atgaacgccc gccatgtgga acctgcttcc cggtcagaat gtaggttaat
    46981 tgtttagcac cgccagaagg atatttggtt ggaatcaccc gcagagaaat atcgtgagag
    47041 tccgccagca ccgcgcgcag catggaaatc gcctgcggtt tgttatcttc aatgccgata
    47101 agaatttcgc gtggctgcag aatatgcgca agaatgcgaa taccctctac gacctgagcc
    47161 gcgcaatcct gcatcaaacg gtcatcggcg gtaatgtacg gctcgcactc agcagcgttg
    47221 ataatcaacg tttcaatctt atctccgcca ccctgcaatt taacgcctgt cgggaatcct
    47281 gcaccgccca gcccggcaac accaaactga tgaatgcgct cgattaactc ttcgcgactg
    47341 cgagagcgat aatcggccca gccgtctcgc gggatccagc agtcttcacc atcggcatca
    47401 ataatcacgc ttaattcagc taacgctgaa ggatgagccg tagagtgggg cgcaatagcc
    47461 gtaacggtac ccgaggtggg cgcgtgaaca ggcagcattt tgccgcgacc acgggtaagc
    47521 ggctggccgc gcaatacttt atcgccgacg ctaacgcaca actcaccttc agcgccaata
    47581 tgctgtttca gtggaataac aaaacgctgc gccagtggta cctggcgcag gggtgtaccg
    47641 ttggactggg ttttcatctc cggtggatgg atgccgccgt tgaaatccca gattttattt
    47701 tttctgaatg cagagaataa cttaagcatg gtgttccacg ggaatgatac gcacgggaat
    47761 ggtgttcaga tcccatttcc aggagtcagg tgtttctgcg accggttgca acgagatgca
    47821 gtgcgtcggg cacggatcaa cacataaatt gcagcccgta cagagatcac tcattaccgt
    47881 atgcatggca cgggtagcgc caacgatggc gtctaccgga caagcctgaa tacatttagt
    47941 gcagccaata cagttatttt catcaataac cgccaccatc cgcgcaggcg ttagctcttg
    48001 cgcttcgcca tccagcggct gcggctcgac attaagcaac tcggcaattt ttagcatcac
    48061 agcttcgcca ccaggggcgc aacggttgat tttttcaccg ttatagctga tggcttccgc
    48121 gtaggggcga cagccgggat aaccgcactg accacactgg ctctgcggta agatttcgtc
    48181 aattttctca acgaccggat cgtcttccac cgcaaaacgg cgggaggcat aacccagaat
    48241 ggcgccaaac gccaggccca gcaggctcac ggcggcaacg gcaatccaga tagcattcat
    48301 tacaacttca ccaaaccact aaagcccata aaggccagag acataagacc tgcggtaatt
    48361 aatgcaatgg cattaccgcg aaaaggtgcc gggacatcag ccacagcaag gcgttcgcgg
    48421 atggcggcga agagcaccat caccagcgag aaaccgacag cggcggaaaa accgtacagc
    48481 gccgactgaa agaaattgtg cccgagattg atattcagca acgccacgcc gagcactgca
    48541 cagttggtgg tgataagcgg caaaaaaatc cccagcagac ggtaaagcac cgggctggtt
    48601 ttgcgcacca ccatctcggt gaactgcaca accacagcaa tcaccagaat aaacgccagg
    48661 gtgcgcaggt aaatcagatt aagcgggatc aaaatccacg tatcgataag ccaggcgcaa
    48721 atagacgcca gcgtcatcac aaacgttgta gccagcccca tgcccatcgc ggtttccagc
    48781 tttttggaaa cccccataaa cggacagaga ccgagaaact tgaccagtac aaagttattg
    48841 accagtacag ttccgacaaa cagtaacagg tagtcagtca tttttcggcc tgaaataaaa
    48901 aagccgccta ttatccgtta atccagagca ggcgacaaca ggttaactgt aaggttatta
    48961 cggattcaca aacgtcgctt tcacccgcga cgagcgttta aaatagggga cgatcaatgc
    49021 ggttgccaac aaagtaaaca gtaactggcg aacggctatg ccatcttcca cgggtgaaaa
    49081 agcgaacgct tttactgcca gcaatacgga aataagcagc cagataatat agtgtttagg
    49141 aacacaacgc cgacgtttga agaaagcgat ggtcaaccac agtgtgtaat accacatggc
    49201 gatagcggta ataaaagaaa cgccccataa gatttgtgtc gtcagtgctt gttcgccgag
    49261 cgtttgaaat gtttgcggag aagataacgc agcggtatac agcaacaacg ccagcgtcgt
    49321 acttaacaga gcgactaaaa gccaggccaa tgggccaagc aaccagcctc caatacgttg
    49381 tggcgttgtg gtggtcatag gttctccata atagcggcgc aaacaggcaa atctaatttt
    49441 aggggcgtta ttataaggct tttataaccg atcgctactt tctttattgg acatagtgcc
    49501 agacggactt cggtacctgg ccgaggtcaa acaaacgccc gccagaaacc agctctgcgc
    49561 gacggtgatc ggcagcacga tacatattga tcagttcctg atcgtccgtc agggtgtaat
    49621 taagatggtc gtagagtttt tccaaacttt cgagtgaact gattttgcga aattttaata
    49681 agtagtcctg aacagtcata aaaggtccat aaaataatta ataccaaagg ggtaggtatt
    49741 attagtgatg ataaaatttc accaaaagtg aacgcttgtg ttattacaat aataagaatg
    49801 cgctgatggc aaatattcag ggatgaaaaa ctgctctgaa aagagtaacg cgctttacta
    49861 tctctgacaa tcaccaacaa catcgaacaa gataataaat tcctggttta atatccgaca
    49921 agtgaaaaca tgcacccgga cgggcagcat gtcgctctac aagcgcagag cttacttgtg
    49981 ttgtaccgta gtactctgtt caggtggctg atagttgtca atgtgactcg ccacgccaag
    50041 aagaatgact gaaacgacaa gaacgatcca acctgttaat tcaataagac gattcattac
    50101 agcccacatc tctgttgatt gatccattaa cttcaggggg taaatgttac ttagcaataa
    50161 tagctccagc aagattttta ctgaggtttt tgcgatattt agcttttgtc gttggaaaat
    50221 tcgctatttt tttgacttaa gttaaacaac atcccttatt gctggcaggt tattaaactg
    50281 ttgagcgagg gtaaaggata gtgtcaaata gccatcatac ttcaatgaga ggcaatgaca
    50341 tgagcgacaa catccgtgtt gggttgattg ggtatggtta tgcgagcaaa accttccatg
    50401 cgcccctgat tgcgggcacg cccgggctgg aactggcggt aatctccagc agcgatgaaa
    50461 caaaagtaaa agccgactgg ccaacggtta cggttgtctc tgagccgaag catctgttta
    50521 acgatcccaa catagacctg attgtcattc ctacacccaa cgatacccat ttcccgttag
    50581 ccaaagcggc gcttgaggcg ggtaaacatg tggtcgttga taaacccttt accgtgacac
    50641 tgtcacaagc gcgagagctg gaagcgctgg caaaaagcct agggcgtgtg ctgtctgtat
    50701 tccataaccg tcgctgggat agcgatttcc tgacgctaaa aggtttgctc gcggaaggcg
    50761 tgctgggtga agttgcttac tttgagtctc attttgaccg cttccgtccg caggttcgcg
    50821 atcgttggcg tgaacagggc ggcccaggca gcggtatctg gtacgattta gcaccgcacc
    50881 ttcttgatca ggccattacg ctatttggtt taccggtcag catgacggta gatttggcac
    50941 agttacggcc cggagcgcag tcgaccgatt atttccacgc catcttgtcc tatccgcagc
    51001 ggcgagtcat tttacacggt accatgctgg cagctgctga gtcagcacgt tatatcgtgc
    51061 atggatcccg aggcagttat gtgaaatatg gcctcgatcc acaggaagaa cgactgaaaa
    51121 atggcgagcg tctgccgcag gaaggctggg gctacgatat gcgtgatggc gtacttaccc
    51181 gcgtggaagg tgaggaacgt atcgaagaaa cgctgttgac agtaccaggg aattatccgg
    51241 cttactatgc ggctattcgt gatgcgttaa atggcgatgg tgaaaatccg gttccggcaa
    51301 gtcaggcaat ccaggtaatg gagttgattg agctgggcat cgaatccgcc aaacatcgcg
    51361 cgacgctgtg ccttgcgtga aaagaggatg gtgcaattag caccatccat ttttgttact
    51421 tcgcggcgac tttttctcgc agtgcgcgtt tttcctctgc gctgaggaaa gccatttcca
    51481 gaccattaat ctgcgcctgg cggatttgct cgcgggataa cccagcagcc ggcgcggcaa
    51541 cggtatattc gtgaatgata tccactccct gtacgccggg atcgtcagtg ttaatgctgg
    51601 cacgaatgcc atgctcaagg aacgttttca gcggatgtgc cgccagctct gccacggtgc
    51661 tggtctgaat attggaggtc agacaggatt caataccaat ttgctgctcg gcgagaaaat
    51721 ccatcagcgc ccgatcttca atggctttta cgccatgtcc aatacgttcc gcccccagtt
    51781 cacgaatcgc ctgccagatg ctttccggcc cggcagcttc gcctgcatgg acggtaatat
    51841 gccagcccgc atcacgcgcg cggttgaagt gagaaaggaa cagacttccc gggaaaccaa
    51901 gttcatcacc ggctaaatca agtgcggtaa tctggtcacg gtgggctaaa aaggcctcca
    51961 gctcttgctg acaggcggct tcgccgaagg tccggctcat aatgccgata agcttcgcct
    52021 gcacaccaaa ggtgcggcaa ccttcacgta cgccatcgat caccgcttcg acaacacccg
    52081 ctacaggcag ctgatgtgcc attgccatgt agcccggtga aaaacgcagc tcgacatagt
    52141 gcaggccatt acgggctgca tcttcaatgt tttcaaatgc cacgcggcgg caagcatcaa
    52201 gagaggcgag aactttaacg ccccagtcaa gtttggtcag aaagctcacc agatcgggtt
    52261 cgttggcaat gacctgaacg tggggaatca gtgtttccag ggattgtgca ggaagcgaga
    52321 tattatactg gcggccaagt tcaagaatgg tctggggacg aatgttgcca tcaaggtggc
    52381 gatggatatc agttaatggc agggtggtat caatcatggt cgcactcttt ttctggatat
    52441 aaaagtgcgc accattataa aaacaaagca gggtaaaaat ccacgattgc gcaacgattt
    52501 tttacgttgc gcaattgggg ttaacgaaca gcgcggatgg cgttaattaa tccagcaaca
    52561 cctttttcca gtttcgaacg cgggcagccg gcattgagac ggacaaaacc acgaccttct
    52621 tcaccgtagg tataccccgg catgatcgcg accttctctt gctcgataag cgctttttgc
    52681 aacgcgttgt cgtcaatatt caatggacgt agatcaagcc aggccagata agtggattgc
    52741 gggatctgcc agttgagttc agggaacgcg gcgttcattt tatctgcgat atacgtcagg
    52801 ttatctttca gatagacgcg taaggcatcc agccacggtg cgccttgctg ataggcggcg
    52861 atatgagcag ttaacgccag taccgaaggg gaagaaagcc catcacgtcc tttcagtgcc
    52921 gataaatagg catcacggct gctgctattt tctataatcc cgtaagcacc ggtcagggcg
    52981 ggaatattga aacttttcga tcccgacgtt agcaatgccc agtctccgcg cgccacatta
    53041 ctccagggaa tatgcggctg ctcgccccaa accatatcca tatggatttc atcggaaata
    53101 acccgcacac catgacgctc gcacaggtca gccatgatct ccagttcatc gcacgtccac
    53161 actttcccgg taggattctg tgggctacac aggagcataa ttttacattc tggtttcgcc
    53221 aacacggctt ccagcttgcc catatcgcaa aaccagccgt cagcccgctt ctctaaagcg
    53281 acgggcatta ctgtgcgctg gttaccttca atggccttgt aaaatgcgtc ataggcgggt
    53341 gtgtggatca ccacgccttc acctttttca gaccactgac gaatcagttc cgagaccatg
    53401 tagataacag aagggccata caccaccgac tgcgtatcaa ttgcggtgta atgctgggtg
    53461 gaaaaccagt gggcaatagc cgcgagaaac tcatcgtttt tccagcggct gtagccaaac
    53521 acgccgtgca tcaggcgctg attcagcgcc tcgataatgc agggggcaga ggcaaaatcc
    53581 atgtctgaaa tcgtgaacgg taacaggtca gcagtgccga aacggtcagc gacataatcc
    53641 cactgcgtac accatgtgcc atgacgatcc acgacctttg aaaaatcgaa catatcttat
    53701 ccttatgcct ggacagtatg catcagaccg gccatttcat cttttactga ctgaacttgt
    53761 ggcccgataa caacctgcag gttatgttga ttaagttgta ctacgccaat tgcccgattg
    53821 tccttcagtg cctgcacatt aacaagcgac atatctttca cagacaaacg cagacgggta
    53881 atgcagttat cgaggctgac aatattgtcg gcaccgccta aggcttcgag gattgccgga
    53941 acgttgtaac ctgatttacc cggcgcaccg gcaacggctt tttcgattga gctggcaact
    54001 tcgctatcgc gccccggggt tttcagattg aagcgggtga tagcgaaacg gaagatgacg
    54061 tagtaaacga caaaccagat tgccgccacc actggcacca tgtaccactt ggttgacaga
    54121 ccatgcaaaa taccgaacac cacgaagtcg atgatattgc cgtcggtatt accgatggtg
    54181 acgccgagca cagacatgac ggtgaagccg aggccggtta acagcgcgtg gatgacatac
    54241 agaactggcg ctacgaacag gaacaggaat tccagcggtt cggtcgtgcc gccaacgaca
    54301 caggcgatca ggccagaaat aagcagacct ttaattttat ggcgattttc cgggcgcgcg
    54361 cagtgataca tagctaacgc tgcacctggc aggccgccga gaaacgcagg cattttacct
    54421 tgcgaaagga aacgcgtggc gctttcagaa aaaccgtgag tggtcgggca actcaattgc
    54481 gcctggaaga tggtcagtgc gccgctgacg gtttgaccgc agacttcctg cgtgccgcct
    54541 gcgtcggtaa agcgaattaa tgccaccaga atgtgatgca gaccaaacgg caacagcaga
    54601 cgttcaccgg taccaaacag catcggtccg aaatcacccg cgctgttgat catatggcct
    54661 aagccgctaa tacccatggc gaaaattggc cagactaatg gaatcaccag accgacaagg
    54721 cccatcacca gcgaggagat gattggcacg aagcgcgtac cgccgaagaa tgccagcgca
    54781 tccggcaggc ggatattatg gaaacgctca tggagcatcc agacgataat accggcgatc
    54841 accgcaccga ggatccctgt atcgatcgac tggatcccaa gaatgctctg aatgttattg
    54901 gctttcagaa ccgcggcgtc cgtggttggc agaatgcctt tattggtcaa ccagaagttt
    54961 accgcgagat tcattaccgc ataaccgaca aagccagcaa atgccgctac acctttgttt
    55021 tcgcgtgcca ggcccagcgg gatggcgata cagaacatga ctggcaggaa actaaaagca
    55081 aacgagccaa tcttactcat ccaggtaaag atagcctgca acacggggtt gcccaggacc
    55141 gggatcaggg tgatgacatc atggctgcta agagaactac caatgccgag cataatgccg
    55201 cagaacgaca ataatgccac aggtagcatg aaggttttgc ctaactgctg gaagaactcc
    55261 cacagcgtga ctttcggtgc tgttttcgcc gtcataaaac gactcctcgt agagaacaat
    55321 ctggattcag taaattgcgc gagaagataa aacgttttat caaattttag tgaggcataa
    55381 atcacattac gcaacgataa tagcgggtat aagataaata aaaggtaaaa cgttttatct
    55441 gtcacataat cagggagtag gtcatctgca tggctaccgc caaaaaaata accattcatg
    55501 atgttgcgct ggctgcgggc gtgtcggtaa gcaccgtttc gctggtgcta agtggcaaag
    55561 ggcgaatctc taccgccaca ggagaacgcg ttaacgccgc cattgaagag ctgggatttg
    55621 tgcgcaatcg tcaggcgtcg gcgctgcgcg gcgggcaaag cggcgtcatt ggtttgatcg
    55681 tccgtgattt atctgcgccg ttttacgccg aattaacggc cggattgacg gaagctctgg
    55741 aagcgcaggg acggatggtt tttttgcttc acggcggtaa agacggcgag cagctggcac
    55801 agcggttttc actgttactg aatcaggggg tcgatggtgt ggtcattgcc ggggctgcag
    55861 gaagcagcga tgacctgcga cggatggcag aagaaaaagc tatcccggtg attttcgctt
    55921 cccgtgccag ttatcttgat gatgttgata cggttcgccc ggataacatg caggctgcac
    55981 agttgttgac ggagcatctc attcgcaatg ggcatcagcg tatcgcctgg ctgggagggc
    56041 aaagttcctc attaacccgg gcagaacggg tggggggcta ttgtgcaact ctactaaaat
    56101 ttggcctgcc gtttcacagc gattgggtgt tggagtgctc ttccagccag aagcaagccg
    56161 cggaagctat cacggcgctt ttacgtcata acccgaccat cagtgctgtg gtttgctata
    56221 acgaaactat tgcgatgggg gcatggtttg gtttgctgaa agcagggcgg caaagcgggg
    56281 aaagcggagt cgatcgttac tttgaacaac aggtttcgtt ggcggcattt accgatgcaa
    56341 cgccaaccac acttgatgat atacctgtta cctgggccag cacgcctgcg cgggaacttg
    56401 gtaccacact tgcggatcgc atgatgcaaa aaatcaccca tgaagagacg cattcacgca
    56461 atcttattat tcccgcccgg ctcattgcgg cgaaataatc ctctctttat ctgctatacc
    56521 tggtagtgtc ctttcctcaa ggttaatgat aaaaaatgtg gcacacaggc ggtgcctttt
    56581 atttttactg aagttcacag gaggtttatg tgtttaattc tgacaacctg agactcgacg
    56641 gaaaatgtgc catcatcaca ggtgccggtg caggtattgg taaagaaatc gccattacat
    56701 tcgcgacagc tggcgcatct gtggtggtca gtgatattaa cgccgacgca gctaaccatg
    56761 ttgttgatga aattcaacaa ctgggtggtc aggcatttgc ctgccgttgt gatattactt
    56821 ccgaacagga actctctgca ctggcagact ttgccgtcag taagctgggt aaagttgata
    56881 tcctggttaa caacgccggt ggcggtggtc ctaaaccgtt tgatatgcca atggcagatt
    56941 ttcgccgtgc ttatgaactg aatgtgtttt cttttttcca tctgtcacaa cttgttgcgc
    57001 cagaaatgga aaaaaatggc ggtggcgtta ttctgaccat cacttctatg gcggcagaaa
    57061 ataaaaatat aaacatgact tcttatgcat catctaaagc tgcggccagt catctggtca
    57121 gaaatatggc gtttgacctg ggtgaaaaaa atattcgggt gaatggcatt gcgccggggg
    57181 caatattaac cgatgccctg aaatccgtta ttacaccaga aattgaacaa aaaatgttac
    57241 agcacacgcc gatcagacgt ctgggccaac cgcaagatat tgctaacgcg gcgctgttcc
    57301 tttgctcgcc tgcagccagc tgggtaagcg gacaaattct caccgtctcc ggtggtgggg
    57361 tacaggagct caattaatac actaacggac cggtaaacaa ccgtgcgtgt tgtttaccgg
    57421 gataaactca tcaacgtctc agctaaataa ctggcagcca aattacggct attggttaac
    57481 caatttccga gtgaaaagta tacgaataga gtgtgccttc gcactattca acagcaatga
    57541 taggcgctca cctgacaacg cggtaaacta gttattcacg ctaactataa tggtttaatg
    57601 atggataaca tgcagactga agcacaaccg acacggaccc ggatcctcaa tgctgccaga
    57661 gagatttttt cagaaaatgg atttcacagt gcctcgatga aagccatctg taaatcttgc
    57721 gccattagtc ccgggacgct ctatcaccat ttcatctcca aagaagcctt gattcaggcg
    57781 attatcttac aggaccagga gagggcgctg gcccgtttcc gggaaccgat tgaagggatt
    57841 catttcgttg actatatggt cgagtctatt gtctctctca cccatgaggc ctttggacaa
    57901 cgggcgctgg tggtcgaaat tatggcggaa gggatgcgta acccacaggt cgccgccatg
    57961 cttaaaaata agcatatgac gatcacggaa tttgttgccc agcggatgcg tgatgcccag
    58021 caaaaaggcg agataagccc agacatcaac acggcaatga cttcacgttt actgctggat
    58081 ctgacctacg gtgtactggc cgatatcgaa gcggaagatc tgtcgcgtga agcgtcgttt
    58141 gctcagggat tgcgggcgat gattggcggt atcttaaccg catcctgatc ctctcatttc
    58201 gcgggctggt gataactgtg cccgcgtttc atatcgtaat ttctctgtgc aaaaattatc
    58261 cttcccgggt tcggagaatt ccccccaaaa tattcactgt agccatatgt catgagagtt
    58321 tatcgttccc attatgctcg aacgaacgtt cggttgctta ttttatggct tctgtcaacg
    58381 ccgttttaaa gattaatgcg atctatatca cgctgtgggt attgcagttt ttggtttttt
    58441 gatcccggtg tcagttcttt ttatttccat ttctcttcca tgggtttctc acagataact
    58501 gtgtgcaaca cagaattggt taactaatca gattaaaggt tgaccagtat ttttatctta
    58561 atgaggagtc ccttatgtta cgtcctgtag aaaccccaac ccgtgaaatc aaaaaactcg
    58621 acggtctgtg ggcattcagt ctggatcgcg aaaactgtgg aattgatcag cgttggtggg
    58681 aaagcgtgtt acaagaaatc cgggcaattg ctgtgccggg aagttttaac gatcagttcg
    58741 ccgatgcaga tattcgtaat tatgtgggca acgtctggta tcagcgcgaa gtctttatac
    58801 cgaaaggttg ggcgggccag cgtatcgtac tgcgtttcga tgcggtcact cattacggca
    58861 aagtgtgggt aaataatcag gaagtgatgg agcatcaggg tggctatacg ccgtttgaag
    58921 ccgatgtcac gccgtatgtt attgccggga aaagtgtacg tatcaccgtt tgtgtgaaca
    58981 atgaactgaa ctggcagact atcccgccgg gaatggtgat taccgatgaa aacggcaaga
    59041 aaaagcagtc ttacttccat gatttcttta actatgccgg gatccatcgc agcgtaatgc
    59101 tctacaccac gccgaacacc tgggtggacg atatcaccgt ggtgacgcat gtcgcgcaag
    59161 actgtaacca cgcgtctgtt gactggcagg tggtagcaaa tggtgatgtc agcgttgaac
    59221 tgcgtgatgc ggatcagcag gtggttgcaa ctggacaagg caccagcggg actttgcaag
    59281 tggtgaatcc gcacctctgg caaccgggtg aaggttatct ctatgaactg tgcgtcacag
    59341 ccaaaagcca gacagagtgt gatatctacc cgctgcgcgt cggcatccgg tcagtggcag
    59401 tgaaggacga acagttcctg attaaccaca aaccgttcta ctttactggc tttggtcgtc
    59461 atgaagatgc ggatttgcgc ggcaaaggat tcgataacgt gctgatggtg cacgatcacg
    59521 cactaatgga ctggattggg gccaactcct accgtacctc gcattaccct tacgctgaag
    59581 agatgctcga ctgggcagat gaacatggca tcgtggtgat tgatgaaact gcagctgtcg
    59641 gctttaacct ctctttaggc attggtttcg aagcgggcaa caagccgaaa gaactgtaca
    59701 gcgaagaggc agtcaacggg gaaacccagc aggcgcactt acaggcgatt aaagagctga
    59761 ttgcgcgtga caaaaaccac ccaagcgtgg tgatgtggag tattgccaac gaaccggata
    59821 cccgtccgca aggtgcacgg gaatatttcg cgccactggc ggaagcaacg cgtaaactcg
    59881 atccgacgcg tccgatcacc tgcgtcaatg taatgttctg cgacgctcac accgatacca
    59941 tcagcgatct ctttgatgtg ctgtgcctga accgttatta cggttggtat gtccaaagcg
    60001 gcgatttgga aacggcagag aaggttctgg aaaaagaact tctggcctgg caggagaaac
    60061 tgcatcagcc gattatcatc accgaatacg gtgtggatac gttagccggc ctgcactcaa
    60121 tgtacaccga catgtggagt gaagagtatc agtgtgcatg gctggatatg tatcaccgcg
    60181 tctttgatcg cgtcagcgct gtcgtcggtg agcaggtatg gaatttcgcc gattttgcga
    60241 cctcgcaagg catattgcgc gttggcggta acaagaaagg gatcttcact cgcgaccgca
    60301 aaccgaagtc tgcggctttt ctgctgcaaa aacgctggac cggcatgaac ttcggtgaaa
    60361 aaccgcagca gggaggcaaa caatgaatca acaactctcc tggcgcgcca tcgtcggcta
    60421 cagcctcggt gacgtcgcca ataacttcgc cttcgcaatg ggggcgctct tcctgttgag
    60481 ttactacacc gacgtcgctg gcgtcggtgc cgctgcggcg ggcaccatgc tgttactggt
    60541 gcgggtattc gatgccttcg ccgatgtctt tgccggacga gtggtggaca gtgtgaatac
    60601 ccgatgggga aaattccgcc cgtttttact cttcggtact gcgccgttaa tgatcttcag
    60661 cgtgctggta ttctgggtac cgaccgactg gagccatagc agcaaagtag tgtatgcata
    60721 tttgacttac atgggcctcg ggctttgcta cagcctggtg aatattcctt atggttcact
    60781 tgctaccgca atgacccaac aaccacaatc ccgcgcccgt ctgggcgcgg ctcgcgggat
    60841 tgccgcttca ttgacttttg tctgcctggc atttctgata ggaccgagca ttaagaactc
    60901 cagcccggaa gagatggtgt cggtatatca tttctggaca attgtgctgg cgattgccgg
    60961 aatggtgctt tacttcatct gcttcaaatc gacgcgtgag aatgtggtac gtatcgtggc
    61021 gcagccgtca ttgaagatca gtctgcaaac cctgaaacgg aatcgcccgc tgtttatgtt
    61081 gtgcatcggt gcgctgtgtg tgctgatttc gaccttcgcg gtcagcgcct cgtcgttgtt
    61141 ctacgtgcgc tatgtgttaa atgataccgg gctgttcact gtgctggtac tggtgcaaaa
    61201 cctggtcggt actgtggcat cggcaccgct ggtgccgggg atggtcgcga ggatcggtaa
    61261 aaagaatacc ttcctgattg gcgctttgct gggaacctgc ggttatctgc tgttcttctg
    61321 ggtttccgtc tggtcactgc cggtggcgtt ggttgcgttg gccatcgcat caattggcca
    61381 gggcgttacc atgaccgtga tgtgggcact ggaagctgat accgtagaat acggtgaata
    61441 cctgaccggc gtgcgaattg aagggctcac ctattcacta ttctcattta cccgtaaatg
    61501 cggtcaggca atcggaggtt caattcctgc ctttattttg ggattaagcg gatatatcgc
    61561 caatcaggcg caaacgccgg aagttattat gggcatccgc acatcaattg ccttagtacc
    61621 ttgcggattt atgctactgg cattcgtcat tatctggttt tatccgctca cggataaaaa
    61681 attcaaagaa atcgttgttg aaattgataa tcgtaaaaaa gttcagcagc aatt
//
LOCUS       DAZGQL010000018        54361 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.018, whole genome shotgun sequence.
ACCESSION   DAZGQL010000018 DAZGQL010000000
VERSION     DAZGQL010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 54361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54361
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.018"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(54..1352)
                     /gene="tig"
                     /locus_tag="ACHKIR_001325"
     CDS             complement(54..1352)
                     /gene="tig"
                     /locus_tag="ACHKIR_001325"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFO9989081.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(1696..2013)
                     /gene="bolA"
                     /locus_tag="ACHKIR_001326"
     CDS             complement(1696..2013)
                     /gene="bolA"
                     /locus_tag="ACHKIR_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HFO9989082.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            2318..2896
                     /gene="yajG"
                     /locus_tag="ACHKIR_001327"
     CDS             2318..2896
                     /gene="yajG"
                     /locus_tag="ACHKIR_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO9989083.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            2940..4415
                     /gene="ampG"
                     /locus_tag="ACHKIR_001328"
     CDS             2940..4415
                     /gene="ampG"
                     /locus_tag="ACHKIR_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HFO9989084.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            4875..5822
                     /gene="cyoA"
                     /locus_tag="ACHKIR_001329"
     CDS             4875..5822
                     /gene="cyoA"
                     /locus_tag="ACHKIR_001329"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HFO9989085.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAAFDQWVAKAKQSPNSMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            5844..7835
                     /gene="cyoB"
                     /locus_tag="ACHKIR_001330"
     CDS             5844..7835
                     /gene="cyoB"
                     /locus_tag="ACHKIR_001330"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HFO9989086.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            7825..8439
                     /gene="cyoC"
                     /locus_tag="ACHKIR_001331"
     CDS             7825..8439
                     /gene="cyoC"
                     /locus_tag="ACHKIR_001331"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HFO9989087.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKVFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVVSWL
                     ALTWLFGAGFIGMELYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            8439..8768
                     /gene="cyoD"
                     /locus_tag="ACHKIR_001332"
     CDS             8439..8768
                     /gene="cyoD"
                     /locus_tag="ACHKIR_001332"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HFO9989088.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            8780..9670
                     /gene="cyoE"
                     /locus_tag="ACHKIR_001333"
     CDS             8780..9670
                     /gene="cyoE"
                     /locus_tag="ACHKIR_001333"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HFO9989089.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            9819..11183
                     /gene="yajR"
                     /locus_tag="ACHKIR_001334"
     CDS             9819..11183
                     /gene="yajR"
                     /locus_tag="ACHKIR_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9989090.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPILTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILAATGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHILLMSTFVALPGQMADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPADTAANEALEVRLLETEGVKEVLIAEEEHSAYVKIDSKVTNRFEVEQA
                     IRQA"
     gene            complement(11311..11802)
                     /gene="yajQ"
                     /locus_tag="ACHKIR_001335"
     CDS             complement(11311..11802)
                     /gene="yajQ"
                     /locus_tag="ACHKIR_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HFO9989091.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            11970..12881
                     /gene="panE"
                     /locus_tag="ACHKIR_001336"
     CDS             11970..12881
                     /gene="panE"
                     /locus_tag="ACHKIR_001336"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFO9989092.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHDVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGITVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            12844..13434
                     /gene="yajL"
                     /locus_tag="ACHKIR_001337"
     CDS             12844..13434
                     /gene="yajL"
                     /locus_tag="ACHKIR_001337"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HFO9989093.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(13488..14936)
                     /gene="thiI"
                     /locus_tag="ACHKIR_001338"
     CDS             complement(13488..14936)
                     /gene="thiI"
                     /locus_tag="ACHKIR_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HFO9989094.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSIDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            15142..15384
                     /gene="xseB"
                     /locus_tag="ACHKIR_001339"
     CDS             15142..15384
                     /gene="xseB"
                     /locus_tag="ACHKIR_001339"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFO9989095.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            15384..16283
                     /gene="ispA"
                     /locus_tag="ACHKIR_001340"
     CDS             15384..16283
                     /gene="ispA"
                     /locus_tag="ACHKIR_001340"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HFO9989096.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLEALERIHRHKTGALIRAAVRLGALSAGDKGRRALSVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            16308..18170
                     /gene="dxs"
                     /locus_tag="ACHKIR_001341"
     CDS             16308..18170
                     /gene="dxs"
                     /locus_tag="ACHKIR_001341"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFO9989097.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEIVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPEAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDATGMEAKIKAWLA"
     gene            18225..19199
                     /gene="yajO"
                     /locus_tag="ACHKIR_001342"
     CDS             18225..19199
                     /gene="yajO"
                     /locus_tag="ACHKIR_001342"
                     /EC_number="1.17.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HFO9989098.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLLRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHGWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYQESNENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHPVVGFK"
     gene            complement(19253..19771)
                     /gene="pgpA"
                     /locus_tag="ACHKIR_001343"
     CDS             complement(19253..19771)
                     /gene="pgpA"
                     /locus_tag="ACHKIR_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFO9989099.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVTAGFVIFRILDMWKPWPIRWFDHNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(19749..20726)
                     /gene="thiL"
                     /locus_tag="ACHKIR_001344"
     CDS             complement(19749..20726)
                     /gene="thiL"
                     /locus_tag="ACHKIR_001344"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HFO9989100.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTLDWKGYDHFATP"
     gene            complement(20804..21223)
                     /gene="nusB"
                     /locus_tag="ACHKIR_001345"
     CDS             complement(20804..21223)
                     /gene="nusB"
                     /locus_tag="ACHKIR_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFO9989101.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(21243..21713)
                     /gene="ribE"
                     /locus_tag="ACHKIR_001346"
     CDS             complement(21243..21713)
                     /gene="ribE"
                     /locus_tag="ACHKIR_001346"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HFO9989102.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(21802..22905)
                     /gene="ribD"
                     /locus_tag="ACHKIR_001347"
     CDS             complement(21802..22905)
                     /gene="ribD"
                     /locus_tag="ACHKIR_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HFO9989103.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRLRAQSHAILTSSATVLADDPALTVRWSELDEQTQGL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGNDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(22909..23358)
                     /gene="nrdR"
                     /locus_tag="ACHKIR_001348"
     CDS             complement(22909..23358)
                     /gene="nrdR"
                     /locus_tag="ACHKIR_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFO9989104.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            23509..24048
                     /gene="yajI"
                     /locus_tag="ACHKIR_001349"
     CDS             23509..24048
                     /gene="yajI"
                     /locus_tag="ACHKIR_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFO9989105.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     KETVKITQQNKLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     MSVDQLGFVRIHDIQPVMQ"
     gene            24325..25230
                     /gene="tsx"
                     /locus_tag="ACHKIR_001350"
     CDS             24325..25230
                     /gene="tsx"
                     /locus_tag="ACHKIR_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HFO9989106.1"
                     /translation="MFKQWHTYEKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQ
                     SVNVVGSYHTRFGPQLRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHG
                     SPLFMEIEPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDI
                     DTGLPMSLSMNVYAKYQWQNYGAANENEWDGYRFKVKYFVPITDLWGGQLSYIGFTNF
                     DWGSDLGDDSGYANNGIKTRTNNSIASSHILALNYDHWHYSVVARYWHNGGQWNDDAE
                     LNFGNGNFNVRSTGWGGYLVVGYNF"
     gene            complement(25268..25615)
                     /gene="yajD"
                     /locus_tag="ACHKIR_001351"
     CDS             complement(25268..25615)
                     /gene="yajD"
                     /locus_tag="ACHKIR_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease YajD"
                     /protein_id="HFO9989107.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(25745..26716)
                     /gene="secF"
                     /locus_tag="ACHKIR_001352"
     CDS             complement(25745..26716)
                     /gene="secF"
                     /locus_tag="ACHKIR_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HFO9989108.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(26727..28574)
                     /gene="secD"
                     /locus_tag="ACHKIR_001353"
     CDS             complement(26727..28574)
                     /gene="secD"
                     /locus_tag="ACHKIR_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HFO9989109.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPSEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(28602..28934)
                     /gene="yajC"
                     /locus_tag="ACHKIR_001354"
     CDS             complement(28602..28934)
                     /gene="yajC"
                     /locus_tag="ACHKIR_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFO9989110.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(28957..30084)
                     /gene="tgt"
                     /locus_tag="ACHKIR_001355"
     CDS             complement(28957..30084)
                     /gene="tgt"
                     /locus_tag="ACHKIR_001355"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFO9989111.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(30139..31209)
                     /gene="queA"
                     /locus_tag="ACHKIR_001356"
     CDS             complement(30139..31209)
                     /gene="queA"
                     /locus_tag="ACHKIR_001356"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFO9989112.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDERINATMTARHGALFEVEFNDQRSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            31302..31883
                     /gene="acpH"
                     /locus_tag="ACHKIR_001357"
     CDS             31302..31883
                     /gene="acpH"
                     /locus_tag="ACHKIR_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HFO9989113.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRNETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFT
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQSVLNGMASRRPRLDALR
                     NSWYDLDAHYDALETCFWQFYPRMMEQASRKAL"
     gene            complement(31888..33705)
                     /gene="malZ"
                     /locus_tag="ACHKIR_001358"
     CDS             complement(31888..33705)
                     /gene="malZ"
                     /locus_tag="ACHKIR_001358"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308480.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004558 - alpha-1,4-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HFO9989114.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EETSVPMHKQRSQPQPGVTAWRAAIDLSGGLPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNLQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYQRMIALRKKSQALRRGGCQVLYAEDNVVVFVRVLYQQRVLVAINRGEACEV
                     VLPASPLLNVAQWQRKEGHGQLTDGILALPAISATVWMN"
     gene            complement(33861..35234)
                     /gene="proY"
                     /locus_tag="ACHKIR_001359"
     CDS             complement(33861..35234)
                     /gene="proY"
                     /locus_tag="ACHKIR_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HFO9989115.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGVAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     SLKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENH"
     gene            complement(35310..36629)
                     /gene="brnQ"
                     /locus_tag="ACHKIR_001360"
     CDS             complement(35310..36629)
                     /gene="brnQ"
                     /locus_tag="ACHKIR_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HFO9989116.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIIWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(37036..38331)
                     /gene="phoR"
                     /locus_tag="ACHKIR_001361"
     CDS             complement(37036..38331)
                     /gene="phoR"
                     /locus_tag="ACHKIR_001361"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HFO9989117.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTHLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKVTRFSFVIPERLIAKNSD"
     gene            complement(38389..39078)
                     /gene="phoB"
                     /locus_tag="ACHKIR_001362"
     CDS             complement(38389..39078)
                     /gene="phoB"
                     /locus_tag="ACHKIR_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HFO9989118.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            39268..40470
                     /gene="sbcD"
                     /locus_tag="ACHKIR_001363"
     CDS             39268..40470
                     /gene="sbcD"
                     /locus_tag="ACHKIR_001363"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HFO9989119.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQAHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITEQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELEESQQQRLQHLFATTLH
                     SLAGEHEA"
     gene            40467..43610
                     /gene="sbcC"
                     /locus_tag="ACHKIR_001364"
     CDS             40467..43610
                     /gene="sbcC"
                     /locus_tag="ACHKIR_001364"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HFO9989120.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISSMVFEQHKSARTELEKLQAQASGVALLTPEQV
                     QSLTASLQVLTDEEKQLLTAQQQEQQSLNWLTRLDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHTRQQIKEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNAWLQEHDRFRQWNNELAGWRAQFSQQTSVREHLRQWQQQLTHA
                     EQKLNALTAITLTLTADEVATALAQHAEQRTLRQRLVALHGQIVPQQKRLAQLQVAIQ
                     NVTQEQTQRNAALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGATLRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQHRQN
                     ELTALQNRMQQLTPILETLPQSDELPHSEETVVLENWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTMEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESAFAVK"
     gene            43736..44920
                     /gene="araJ"
                     /locus_tag="ACHKIR_001365"
     CDS             43736..44920
                     /gene="araJ"
                     /locus_tag="ACHKIR_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HFO9989121.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGKLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNVLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFFGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKP
                     LG"
     gene            complement(45059..45967)
                     /gene="mak"
                     /locus_tag="ACHKIR_001366"
     CDS             complement(45059..45967)
                     /gene="mak"
                     /locus_tag="ACHKIR_001366"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HFO9989122.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVPQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            46092..47003
                     /gene="rdgC"
                     /locus_tag="ACHKIR_001367"
     CDS             46092..47003
                     /gene="rdgC"
                     /locus_tag="ACHKIR_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HFO9989123.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(47084..47164)
                     /locus_tag="ACHKIR_001368"
     CDS             complement(47084..47164)
                     /locus_tag="ACHKIR_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9989124.1"
                     /translation="MTQRPWSKLQHKTHNIAALRIIARCS"
     gene            complement(47161..47490)
                     /locus_tag="ACHKIR_001369"
                     /pseudo
     CDS             complement(47161..47490)
                     /locus_tag="ACHKIR_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(47647..47931)
                     /gene="ppnP"
                     /locus_tag="ACHKIR_001370"
     CDS             complement(47647..47931)
                     /gene="ppnP"
                     /locus_tag="ACHKIR_001370"
                     /EC_number="2.4.2.1"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HFO9989125.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSLFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(48003..48680)
                     /gene="aroM"
                     /locus_tag="ACHKIR_001371"
     CDS             complement(48003..48680)
                     /gene="aroM"
                     /locus_tag="ACHKIR_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HFO9989126.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNHLAHVSRRKVERDLQGVVEVLDNQGYDVIILMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEELLTVQAQKWQILQKPPVFSL
                     GNPIHDSEQKIIDVGKELLAKGADVIMLDCLGFNQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLM"
     gene            complement(48938..49129)
                     /gene="yaiA"
                     /locus_tag="ACHKIR_001372"
     CDS             complement(48938..49129)
                     /gene="yaiA"
                     /locus_tag="ACHKIR_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HFO9989127.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(49179..49703)
                     /gene="aroL"
                     /locus_tag="ACHKIR_001373"
     CDS             complement(49179..49703)
                     /gene="aroL"
                     /locus_tag="ACHKIR_001373"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HFO9989128.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(49886..50344)
                     /gene="yaiI"
                     /locus_tag="ACHKIR_001374"
     CDS             complement(49886..50344)
                     /gene="yaiI"
                     /locus_tag="ACHKIR_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFO9989129.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            50464..51273
                     /gene="proC"
                     /locus_tag="ACHKIR_001375"
     CDS             50464..51273
                     /gene="proC"
                     /locus_tag="ACHKIR_001375"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFO9989130.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVTA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGITL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGTLKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(51290..52405)
                     /gene="adrA"
                     /locus_tag="ACHKIR_001376"
                     /gene_synonym="dgcC"
     CDS             complement(51290..52405)
                     /gene="adrA"
                     /locus_tag="ACHKIR_001376"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HFO9989131.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPSPGWWWLLLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNALPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVIYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVHEGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(52507..52827)
                     /gene="psiF"
                     /locus_tag="ACHKIR_001377"
     CDS             complement(52507..52827)
                     /gene="psiF"
                     /locus_tag="ACHKIR_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HFO9989132.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(52946..>54361)
                     /gene="phoA"
                     /locus_tag="ACHKIR_001378"
     CDS             complement(52946..>54361)
                     /gene="phoA"
                     /locus_tag="ACHKIR_001378"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HFO9989133.1"
                     /translation="VKQSTIALALLPLLFSPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATVGEWQGKTLREQAQARGYQLVSDAASLNAVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKDGNTLVIVTADH
                     AHASQIVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
BASE COUNT        13067 a        14536 c        13717 g        13041 t
ORIGIN      
        1 ccacctggcg gtgacgggcc tttgtgcgaa tttagcgcgt tatgctgcgt aaattacgcc
       61 tgctggttca tcagctcgtt gaaagtggtt tctttttcag tcactttcgc tttcgccagt
      121 acagcttcaa cagcctgttc ttccagagca acattgcgca tgttgtccat cagttctttg
      181 tttttgctgt agaactcgat aacttctttc ggatcttcgt acgcagaagc catctcttcg
      241 atcaggcctt tcacgcgctc ttcgtcagct ttcagctcgt tggtgcggat aacttcgccc
      301 agcagcaggc caacaactac gcgacgttta gcctgttctt caaacagttc acgcggcagt
      361 tccagagctt gtttttcgtt gccaccgaaa cgctgtgcag cctggcgacg cagaacgtcg
      421 atttcgctgt cgatcagcgc agccggtacg tcgatgtcgt tagcttttac cagaccttcg
      481 atcgcctgag acttaacgcg gttacggatg gcgctcttca gctcgcgctc catgttttta
      541 cgcacttcag cgcgcagacc ttctacggaa ccatcttcaa cgccgaaacg tttgatgaat
      601 tctgcagtca gttccggcag ttcacgctct tcaactttct tcaggttgat agcgaatttc
      661 gctgctttac ctttcaggtt ttctgcgtgg tattcttccg ggaaggtcac gtcgatggtg
      721 aactcttcgc cagctttgtg gcctttgata ccgtcttcaa agcccggaat catacgcccc
      781 tggcccatcg ccagtacgaa atcagacgct ttaccgcctt cgaactcttc gccgtctaca
      841 gaaccggtga agtcgatggt tacgcggtct tctgcttcaa cagcgccgtc tttttctttc
      901 caggtcgcct gctgtttacg cagagtatcc agcatgccgt caacgtcagc gtcggtcact
      961 tcaacgatcg gtttttcaac ttcgatcgct tccagaccct gcagttcaac ttccggataa
     1021 acttcaaact ctacagagta ggtgaagtct tcacccagct tgtattcgcc cggaacataa
     1081 gtcggtgcgc cagccggatt gattttttct ttaatgatgg cgtcaatgaa gttacggctc
     1141 atcaggtcac ccagaacgtc ctggcgtaca gacgcaccat aacgctgagc aacgatattc
     1201 attggcactt tgcctttgcg gaagccgtca atacgtactt ttttcgcaac gttgaccagc
     1261 tcgcttttaa cagcggtctc gatgctgtca gcagcgatag taatcgttac acggcggcca
     1321 aggccttgag tggtttcaac tgaaacttgc atcttgttac ctcaaaaaat cacagtgctc
     1381 ggtcaactct taccccgcaa gcacaggttg ttggcttcgc ggaaccggga tgttcttata
     1441 atcaatcaca ttccctgtta ccagaatcat cccgaaagca ttcaaatagg acgcggcatt
     1501 atagcggcat cacttttatg agtcgagaac ggttattgcg cgctgtcgcg tattttttca
     1561 caattcccgg gcaattttcg tctgaatgct gggcaaaaag cgcaaaatcc agacaaaaca
     1621 aaacggcccg caggccgttt ttcataaaat ctttagcaac atactggaaa agcgccgaca
     1681 gttgcaaatg cgtttttacg cgatgcttcc tgctccacga cagggaggag aggcaaagac
     1741 ggtgtcctgc aacccttccc actccttaat agtgtaagta tgcagagcca gcgcatgaac
     1801 ggtagtagag agttcctccg ctaaagtact gtaaatcatt cgatgacgat tcagaaaacg
     1861 ttcacccgta aaacgatcgc tgaccagcac aactttaaaa tggctttcag agccggctgg
     1921 gacattgtga cgatagcttt catccactac ttcgaggaat acgggttgga acgccgccct
     1981 taatttttct tctatccgct cacgtatcat catgaaatat ctcctccgac aacgctgaga
     2041 tgtcatccat ccctttaaat actagccgct tttaccgttt ccattgcagc ttaacaacaa
     2101 atatttaggt ttcgtcctgg tttttctgtc aagcgtatga agtttactga tttaaagatt
     2161 gaaacacagg cctcgcatca gcgtttcttt taccaggggc gattatttcg tcaacatgat
     2221 gaatttacat gccttaccca cttcccctcg ccgttggcaa tgttatgatg gcgggaattt
     2281 ttaccaaaca cgcttaagag tctctgagag cccgaacatg ttcaaaaaaa tcctcttccc
     2341 gttagttgct ctgtttatgc ttgcaggatg cgcaaaaccg ccaacaacta ttgaagtttc
     2401 cccgacgatt acactgccac agcaggatcc aagcctgatg ggcgtcaccg taagcattaa
     2461 tggtgccgat cagcgtaccg atcaggcgct ggcaaaagtc acccgcgata atcaaatcgt
     2521 taccctgacc gcctcccgcg atctgcgttt cctgctgcaa gaagtgctgg aaaaacaaat
     2581 gaccgcccgt ggatacatgg ttggtccgaa tggcccggtt aatctgcaaa tcatcgttag
     2641 ccaactgtat gctgacgtgt cccagggcaa cgtgcgctac aacatcgcga ccaaagcgga
     2701 tatcgccatc atcgctaccg cgcagaatgg caacaaaatg accaaaaact atcgtgccag
     2761 ctacaacgtt gaaggtgcgt tccaggcctc caacaaaaat atcgccgatg cggttaacag
     2821 tgtgctgacc gataccatcg ctgatatgtc tcaggacacc agcatccacg aattcatcaa
     2881 gcagaacgcg cgttaatttc tgccctctgg cccggtgcaa gccgggcctg tagacgccca
     2941 tgtccagtca atatttacgt atttttcaac agccgcgttc agccatattg ctgatcctgg
     3001 gttttgcttc cgggctaccg ctcgccctga cgtccggcac cttacaggcc tggatgacgg
     3061 tcgagaatat cgatctcaaa accattggtt tcttctctct ggtaggccag gcttacgttt
     3121 ttaaattcct ctggtcacca ctgatggacc gctacacgcc cccatttttc gggcggcggc
     3181 gcggttggct gctcgccacg caaattctgt tattagtcgc cattgcggcg atgggttttc
     3241 tcgaaccagg cacccagctc cgctggatgg cggcactggc ggtggtgatc gctttttgct
     3301 ctgcctccca ggatattgtc ttcgatgcgt ggaaaaccga tgtgctcccg gcagaagaac
     3361 gtggtgcggg cgcggcaatc agcgtgctgg gttaccgttt agggatgctg gtttccggcg
     3421 gcttagccct gtggctggca gataaatggc tgggctggca gggcatgtac tggttgatgg
     3481 cggcgctgtt gatcccctgt attatcgcga cgttgcttgc accagaaccc accgacacca
     3541 ttcctgtgcc caaaacgctg gaacaagcgg ttgttgcacc tctgcgagat ttctttggtc
     3601 gcaataatgc ctggcttatt ttgcttctta tcgtgctgta taagctgggc gacgcattcg
     3661 ctatgagcct gacaaccacg tttttgattc gcggcgtcgg gtttgatgcg ggtgaagtag
     3721 gcgtggttaa caaaacgctt ggcttactgg cgaccattgt tggcgcattg tacggtggga
     3781 ttttgatgca gcgcctgtcg ctgttccgtg cactgctgat tttcggcatt ttacaaggtg
     3841 catctaacgc cggttactgg ctgctgtcga ttactgataa gcatctctac agcatgggcg
     3901 cagccgtctt tttcgaaaac ctctgtggcg ggatgggtac atcagccttt gtcgcgctgt
     3961 taatgacgct gtgtaataag tcattttccg ctactcaatt tgccctgctc tcagcgcttt
     4021 ctgctgtagg gcgagtttat gtcggccccg tggcgggttg gtttgttgaa gcacacggct
     4081 ggtcgacatt ctatctattc tccgtcgccg ctgccgtacc agggcttatc ttgctgctgg
     4141 tttgtcgcca gacgcttgaa tatacacgag taaatgataa ctttatctcc cgtaccgaat
     4201 atccggcagg ttatgccttt gccatgtgga cactggcggc gggcgtcagc ctgttggccg
     4261 tgtggttact gctgttgacg atggacgcgc tggatttgac gcacttctct ttcctgcctg
     4321 ctctgctgga agtcggggtt ttagtcgccc tttctggcgt cgtgcttggt ggtttactgg
     4381 attatctggc gctacgaaaa acgcatctga cgtaatctgt aaatattatt tagccgtagt
     4441 aatcactcgc cgtaattatt acggctaaat aaacatcagc atcgcttatt aatatttgtt
     4501 cttttgtgcg gcttagcgtc tggaaattat tgacgccatt tatgaattct atttttctta
     4561 cacgattcag ctaatgagtc tttattttcc cactacccag ttgtcacctt aatgataatt
     4621 atttgttaaa taattgtttt atttcgcatt ggttatacca attgcccgcc cagactcctt
     4681 ctgcactccc cttttgttat aacgcccttt tgcaacagct tcttaaaatc aacctgatat
     4741 gttttgcaac atatgtgacc tgtaagccaa atccaagtaa caggaattta atcatgttta
     4801 cagtaatgta accttcccgt aaaatgccca cacactttaa acgccaccag atcccgtgga
     4861 attgaggtcg ttaaatgaga ctcaggaaat acaataaaag tttgggatgg ttgtcattat
     4921 ttgcaggcac tgtattgctc agtggctgta attctgcgct gttagatccc aaaggacaga
     4981 ttggtctgga gcaacgttca ctgatactga cggcatttgg cctgatgttg attgtcgtta
     5041 ttcccgcaat cttgatggct gttggtttcg cctggaagta ccgtgcgagc aataaagatg
     5101 ctaagtacag cccgaactgg tcacactcca ataaagtgga agctgtggtc tggacggtac
     5161 ctatcttaat catcatcttc cttgcggtac tgacctggaa aaccactcac gctcttgagc
     5221 ctagcaagcc gctggcacac gacgagaagc ccattaccat cgaagtggtt tccatggact
     5281 ggaaatggtt cttcatctac ccggaacagg gcattgcaac cgtgaatgaa atcgctttcc
     5341 cggcgaacac tccggtgtac ttcaaagtga cctccaactc cgtgatgaac tccttcttca
     5401 ttccgcgtct gggtagccag atttatgcca tggccggtat gcagactcgc ctgcatctga
     5461 tcgccaacga acccggtact tatgacggta tctccgccag ctacagcggc ccgggcttct
     5521 caggcatgaa gttcaaagct attgcaacac cggatcgcgc cgcattcgac caatgggtcg
     5581 caaaagcgaa acagtcgccg aacagcatgt ctgacatggc agcgttcgaa aaactggccg
     5641 cgcctagcga atacaaccag gtggaatatt tctccaacgt gaaaccagac ttgtttgccg
     5701 atgtgattaa caagtttatg gctcacggta agagcatgga catgacccag ccagaaggtg
     5761 agcacagcgc acatgaaggt atggaaggca tggacatgag ccacgcggaa tccgcccatt
     5821 aaaggggttg aggaagaata aagatgttcg gaaaattatc acttgatgca gtcccgttcc
     5881 atgaacctat cgtcatggtt acgatcgctg gcattatttt gggaggtctg gcgctcgttg
     5941 gcctgatcac ttacttcggt aagtggacct acctgtggaa agagtggctg acctccgtcg
     6001 accataaacg cctcggtatc atgtatatca tcgtggcgat tgtgatgttg ctgcgtggtt
     6061 ttgctgacgc cattatgatg cgtagccagc aggctcttgc ctcggcgggc gaagcgggct
     6121 tcctgccacc tcaccactac gatcagatct tcaccgcgca cggcgtgatt atgatcttct
     6181 tcgtggcgat gcctttcgtt atcggtctga tgaacctggt ggttccgctg cagatcggcg
     6241 cgcgtgacgt tgcgttcccg ttcctcaaca acctaagctt ctggtttacc gttgttggtg
     6301 tgattctggt taacgtttct ctcggcgtgg gcgaatttgc gcagaccggc tggctggcct
     6361 atccaccgct atcgggaata gagtacagtc cgggagtcgg tgtcgattac tggatatgga
     6421 gtctccagct atccggtata ggtacaacgc ttaccggtat caacttcttc gttaccattc
     6481 tgaagatgcg cgcaccgggc atgaccatgt tcaagatgcc agtatttacc tgggcatcac
     6541 tgtgcgcgaa cgtactgatt attgcttcct tcccaattct gacggttacc gttgcgttgt
     6601 tgaccctgga tcgctatctg ggcacccatt tctttaccaa cgatatgggt ggcaacatga
     6661 tgatgtacat caacctgatt tgggcctggg gccacccgga agtttacatc ctgatcctgc
     6721 ctgttttcgg tgtgttctcc gaaattgcgg caaccttctc gcgtaaacgt ctgtttggtt
     6781 atacctcgct ggtatgggca accgtctgta tcaccgtgct gtcgttcatc gtctggctgc
     6841 accacttctt tacgatgggt gcgggcgcga acgtaaacgc cttctttggt atcaccacta
     6901 tgattatcgc catcccgacc ggggtgaaga tcttcaactg gctgttcacc atgtatcagg
     6961 gccgcatcgt gttccattct gcgatgctgt ggaccatcgg ttttatcgtc accttctcgg
     7021 taggcggtat gactggcgtg ctgctggcag taccgggcgc ggacttcgtt ctgcataaca
     7081 gcctgttcct gattgcgcac ttccataacg tgatcatcgg cggcgtggtc ttcggctgct
     7141 tcgcagggat gacctactgg tggcctaaag cgttcggttt caaactgaac gaaacctggg
     7201 gtaaacgcgc gttctggttc tggatcatcg gcttcttcgt tgcctttatg ccgctgtatg
     7261 cgctgggctt catgggcatg acccgtcgtt tgagccagca gattgacccg cagttccaca
     7321 ccatgctgat gattgcagcc agcggtgcag tactgattgc gctgggtatt ctctgcctcg
     7381 ttattcagat gtacgtttct attcgtgacc gcgaccagaa ccgtgacctg actggcgacc
     7441 cgtggggtgg ccgtacgctg gagtgggcaa cctcttcccc gcctccgttc tataactttg
     7501 ccgttgtgcc gcacgttcac gagcgtgatg cattctggga aatgaaagag aaaggcgaag
     7561 cgtacaaaaa gcctgaccac tatgaagaaa ttcatatgcc gaaaaacagc ggtgcaggta
     7621 tcgtcattgc ggctttctcc accatcttcg gtttcgccat gatctggcat atctggtggc
     7681 tggcgattgt tggcttcgca ggcatgatca tcacctggat cgtgaaaagc ttcgacgagg
     7741 acgtggatta ctacgtgccg gtggcagaaa tcgaaaaact ggaaaaccag catttcgatg
     7801 agattactaa ggcagggctg aaaaatggca actgatactt tgacgcacgc gactgcccac
     7861 gcgcacgaac acgggcacca cgatgcaggc ggaaccaaag tcttcggatt ctggatctac
     7921 ctgatgagcg actgcattct gttctctatc ctgtttgcta cctatgccgt tctggtgaac
     7981 ggcaccgcag gcggcccgac aggtaaggac attttcgaac tgccgttcgt tctggttgaa
     8041 actttcctgc tgttgttcag ctccatcacc tacggcatgg cggctatcgc catgtacaaa
     8101 aacaacaaaa gccaggttgt ctcctggctg gcgttgacct ggttgtttgg cgccggattt
     8161 atcgggatgg agctctatga attccatcat ctgattgtta acggcatggg tccggatcgc
     8221 agcggcttcc tgtcagcgtt ctttgcgctg gtcggcacgc acggtctgca cgtcacttcc
     8281 ggtcttatct ggatggcggt gctgatggtg caaatcgccc gtcgcggcct gaccagcact
     8341 aatcgtaccc gcatcatgtg cctgagcctg ttctggcact tcctggatgt ggtttggatc
     8401 tgtgtattca ctgttgttta tctgatgggg gcgatgtaat gagtcattct accgatcaca
     8461 gcggcgcgtc ccatggcagc gtaaaaacct acatgacagg ctttatcctg tcgatcattc
     8521 tgacggtgat tccgttctgg atggtgatga caggagctgc ctctccggcc gtaattctgg
     8581 gaacaatcct ggcaatggca gtggtacagg ttctggtgca tctggtgtgc ttcctgcaca
     8641 tgaataccaa atcagatgaa ggctggaaca tgacggcgtt tgtcttcacc gtgctaatca
     8701 tcgctatcct ggttgtaggc tccatctgga ttatgtggaa cctcaactac aacatgatga
     8761 tgcactaaga gcggcggtta tgatgtttaa gcaatacctg caagtaacga aaccaggcat
     8821 catctttggc aacctgatct cggtgattgg gggattcctg ttggcctcaa agggcagcat
     8881 tgattatccc ctgtttatct acacgctggt tggggtgtca ctggttgtgg cgtcgggttg
     8941 tgtgtttaac aactacatcg acagggatat cgacagaaag atggaaagga cgaagaatcg
     9001 ggtgctggtt aaaggcctga tctcacctgc tgtctcgctg gtgtacgcca ccttgctggg
     9061 tattgctggc tttatgctgc tgtggtttgg cgcgaatccg ctggcctgct ggctgggggt
     9121 gatgggcttt gtggtttatg tcggcgttta tagcctgtac atgaaacgcc actcagtcta
     9181 cggcacattg attggttcgc tctccggcgc tgcgccgccg gtgatcggct actgtgcagt
     9241 aaccggcgag ttcgatagtg gcgcagcgat cctgcttgct atcttcagtc tgtggcagat
     9301 gcctcactcc tatgccatcg ccattttccg ctttaaggat taccaggcgg caaacattcc
     9361 ggtattgcca gtggtaaaag gcatttcggt ggcgaagaat cacatcacgc tgtatatcat
     9421 cgcctttgcc gttgccacgc tgatgctctc tcttggcggt tacgctgggt ataaatatct
     9481 ggtagtcgcc gcggcggtga gcgtctggtg gttaggcatg gctctgcgcg gttataaagt
     9541 tgctgatgac agaatctggg cgcgcaagct gttcggcttc tctatcatcg ccatcactgc
     9601 cctctcggtg atgatgtccg ttgattttat ggtgccggac tcgcatacgc tgctggctgc
     9661 tgtgtggtaa cacaacctct cagttaaaaa ggtgctacgg cacctttttt cttagcatta
     9721 gaatcatatc cctctcgaaa tatttactaa aaaatccgca tgtttacccc attcgtttgc
     9781 cgctttacac tagtcgcgaa tttaaaacag aggtggtaat gaacgattat aaaatgacgc
     9841 caggtgagcg acgcgcgacc tggggtttag ggaccgtatt ctcgttgcgc atgctgggca
     9901 tgttcatggt tctgccgatt ctgaccacgt acggcatggc tctgcaaggt gccagcgaag
     9961 cattaatcgg tattgccatt ggtatttatg gtctgactca ggccgttttt cagattccgt
    10021 ttggcctgct ttccgaccgc attggtcgca aaccattaat tgtcggtggg ctggcagtgt
    10081 ttgccgccgg tagcgttatc gctgcgctct ctgactccat ctggggaatt attctgggcc
    10141 gggcgctaca aggctccggt gcgattgccg ctgccgttat ggcgctgctt tccgatctca
    10201 cgcgcgaaca aaaccgcacc aaagcgatgg cgtttatcgg cgtgagcttt ggcattacct
    10261 ttgccattgc gatggtgctt ggcccgatca tcactcacaa acttgggctg cacgcgctgt
    10321 tctggatgat cgctattctg gcagcaaccg gcattgcgtt gaccatttgg gttgtgccca
    10381 acagtagcac tcacgtactt aatcgtgagt ccggaatggt gaaaggaagt ttcagtaaag
    10441 tgctggcgga accgcggctg ctgaaactca actttggcat tatgtgtctg catattttgc
    10501 tgatgtcgac ctttgttgcc ctgcccggac agatggctga tgcggggttc ccggcggctg
    10561 aacactggaa ggtctacctg gcaacgatgt taatcgcctt tggctcggtc gtgcctttca
    10621 ttatctacgc tgaagttaag cgcaaaatga agcaagtctt tgtcttttgc gtcgggctga
    10681 tcgtggttgc ggaaattgtg ttgtggaacg cacaaacgca gttctggcaa ctggtggtcg
    10741 gcgtgcagct tttctttgtg gcgtttaatt tgatggaagc ccttctgcct tcacttatca
    10801 gtaaagaatc gccagcaggt tacaaaggta cggcgatggg tgtttactcc accagccagt
    10861 ttcttggcgt ggcgattggc ggttcgctgg gcggctggat tgacggcatg tttgacggtc
    10921 agggagtatt tctcgctggc gcaatgctgg ccgcagtgtg gctggcagtc gccagtacca
    10981 tgaaagaacc gccgtatgtc agcagcttgc gcattgaaat cccggcggac actgccgcaa
    11041 acgaagcgtt agaagtacgt ttgctggaaa ctgaaggcgt caaagaagtg ttgattgcag
    11101 aagaagaaca ttcagcgtat gtgaaaatcg acagcaaagt gacgaatcgc tttgaggttg
    11161 aacaggcaat tcgccaggca taaaagcgag gcgaaattgc ctgctgcgct acgcttatca
    11221 ggcctactaa gttcctgagt tacaaacctt tgtaagccaa atcaggcgtt catgccgcat
    11281 ctgacatgaa caaagcgcag gttgtcgcga ttaatcgcgg aagtttttga actggaacgg
    11341 ctgaccgaga tcgccaccac gtaccatcgc catgacagcc tgcaaatcat cacgagattt
    11401 acccgttacg cggatctcat cgccctgaat ttgcgcctgc actttcagtt tgctgtcttt
    11461 gatcattttg acgattttct tctgagtcgc gctctcaatg ccctgtttca gtttcgcttc
    11521 cacaaaccag gttttaccgc tatgaacgat attttccggt acatccagcg aactgccttc
    11581 aatgccgcgc ttcagcagct tggcacgcag aatatccagc aactgattga cctggaaatc
    11641 ggactcgctc aacactttga tggttttgct ggcgtcgttc agctcaaatg aggcttcaac
    11701 attacggaag tcaaaacggg actccacttc gcggctcgcg ttatcgaccg cgttacgtgc
    11761 ttcctgaaga tcaacttcag agacaatatc gaaagatggc atcttttcct ctcccttcat
    11821 ttttgatgcg aagcataata cctgcaaagt taaataacca acagtcctgc accaggacgt
    11881 atcccttaat tgtgcatagt cgcaacagca tgcacaatta agggatagcc ttattgaaca
    11941 cccggagtgg ttgcgggtga ggaggaacaa tgaaaattac cgtattggga tgcggtgcct
    12001 tagggcaatt atggcttaca gcactttgta aacagggtca cgacgttcag ggctggctgc
    12061 gcgtaccgca accttattgt agcgtgaatc tggttgagac agatggttcg atatttaacg
    12121 aatcgctgac cgccaacgat cccgattttc tcgccaccag cgatctgctc ctggtgacgc
    12181 tgaaagcatg gcaggtttcc gatgccgtca aaagcctcgc gtccacactg cctgtaacta
    12241 cgccaatact gttaattcac aacggcatgg gcaccatcga agagttgcaa aacattcagc
    12301 agccattact gatgggcacc accacccatg ccgctcgccg cgacggcaat gtcattattc
    12361 atgtggcaaa cggtatcacg catattggtc cggcacggca acaggatggc gattacagtt
    12421 atctggcgga tattttgcaa accgtgttgc ctgacgttgc ctggcataac aatattcgcg
    12481 ccgagctgtg gcgcaagctg gcagtcaact gtgtgattaa tccactgact gccatctgga
    12541 attgcccgaa cggtgaatta cgtcatcatc cgcaagaaat tatgcagata tgcgaggaag
    12601 tcgcggcagt gatcgaacgc gaaggacatc atacttcagc agaagatttg cgtgattacg
    12661 tgatgcaggt gattgatgcc acagcggaaa atatctcgtc gatgttgcag gatatccgcg
    12721 cgctgcgcca caccgaaatc gactatatca atggttttct cttacgccgc gcccgcgcgc
    12781 atgggattac cgtaccggaa aacacccgcc tgtttgaaat ggtaaaaaga aaggagagtg
    12841 aatatgagcg catcggcact ggtttgcctc gcccctggta gtgaagagac tgaagccgtc
    12901 accactatcg atctgctggt tcgcggcggt atcaaagtca ccactgccag cgtcgccagc
    12961 gatggcaacc tggcgattac ctgctcgcgc ggcgtgaagc tgctggcgga tgcgccgctg
    13021 gtcgaagtag ctgatggcga atatgacgtg atcgtgctgc ctggaggcat taaaggcgca
    13081 gagtgttttc gcgatagcac cctgctggtt gaaaccgtta aacagttcca ccgttccggg
    13141 cgtatcgtcg cggctatttg cgccgcacca gccaccgtgc tggtcccgca cgatatcttc
    13201 ccgattggca atatgaccgg cttcccgacg ctgaaagaca aaatacccgc cgaacaatgg
    13261 caggacaagc gcgtcgtctg ggatgcacgg gtaaaattgc tgaccagtca ggggccgggt
    13321 acagctatcg actttggtct gaaaattatc gacctgctgg ttgggcgtga aaaagcccat
    13381 gaagtggcat cacaactggt gatggcggca gggatttata attattacga gtagtggcgg
    13441 atgcggcaaa cgtcgcatct gaccagatgc gacgtaaaaa ccacaaatta cgggcgatat
    13501 accttcacat tgttaaagcc ctgctcgcgc agatagagcg cctgcaggcg gctcatcacc
    13561 ccgcgctcac accacaacag ccaggttttg ttctggtcga gatcgccaaa tttggtgctc
    13621 agtttataga acggcagaga aaccacatca atcccttcga ctttcagcgg cttatcttcc
    13681 tgttcatcga tagaacggat atcgaggatc acgtcgttcg ggccgaagcc attgacggtt
    13741 tccacttcca ccacttcctg ctcggtctgc tgggcgattt cgcggatatc aacgttattc
    13801 gcttcctcaa ccactttatc gagaatgctg aagtcgaact tctcttcttc cgcttcaatc
    13861 ttcgatttaa ccgctttcac cgtcgggctt ttggagatca caccgcaata ttcgggcatc
    13921 gtgcgggcaa agtcttcagt gccaatctgg cgagccaggt tgatgatgtg ctctttgtcg
    13981 taagaaatca gcggacgcag gatcagcgtg tcggagacgt tatcaatcag gcgcaggtta
    14041 gtcagcgtct ggctggacac ctggcccagt gcttcgccag tgaccagcgc ctgtacaccg
    14101 taacgttcag ccactttaga cgcggcacgc accatcatac gtttgaggat aacacccatc
    14161 tgaccgtcgt cgattttctc gagaatttcc ccgacgacag gttcgaaatt aatagcgaca
    14221 aaacgcacgc ggtgggagct gccaaagcgg ttccacagat aatgcgccac ctgacgcacg
    14281 ccaatttcat gcgccgcgcc gccaaggtta aagaagcagt aatgcacacg acatccgcga
    14341 cgcatcaaca tataactgga aacgccggag tcaaaaccac cggaaatgag cgacagcaca
    14401 tcttcctggg taccaatcgg gaaaccgcca ataccttcgt agcgaccttt aatcagcagg
    14461 agacgatcgt cttccacttc cagatggaca gtcacatccg gattggtcag cttcacgcgc
    14521 gcggactcaa tatgctgatt taaaccgccg ccgacgtaac gttccacatc aatcgagcta
    14581 aaatcatgtt tgccacggcg cttcacgcgc acgcagaagg ttttgccttc cagctgatcg
    14641 cgatactgaa ccaacgcttt ctcgaaaata tcgtgcatgt cggtaaacgg cacgtcttcg
    14701 acttcgagaa tatggtggat gcccggaata cgggtcaggg cgtcgcgaat agccagacgc
    14761 tggttttcat cttttgcgcg aacttcgatg ttatcccagt ggcggacgac agcgagcgtc
    14821 tcatcatagt gctttaaaac gttacgaatg ttcccggtaa ggatttttat aaagcgcaag
    14881 cgcacagatt ggcttttgat ggtgatttcc gggaacaatt taatgataaa cttcatggcg
    14941 gcaatggttc gttggcaagc cttaagcgac ttgtataggg aaaaatacag cagcccacac
    15001 ctgcggctgc atccaggcgc ggaagtatac cactaacatc gctttgctgc gcacatcacc
    15061 ttaccattgc gcgttatttg ctatttgccc tgagtccgtt accatgacgg ggcgaaaaat
    15121 attgagagtc agacattcat tatgccgaag aaaaatgagg cgcccgccag ctttgaaaag
    15181 gcgctgagcg agctggaaca gattgtaacc cgtctggaaa gtggcgacct gccgctggaa
    15241 gaggcgctga acgagttcga acgcggcgtg caactggcac gtcaggggca ggccaaatta
    15301 caacaagccg aacagcgcgt gcaaattctg ctgtctgaca atgaagacgc ctctctaacc
    15361 ccttttacac cggacaatga gtaatggact ttccgcagca actcgaagcc tgcgttaagc
    15421 aggccaacca ggcgctgagc cgttttatcg ccccactgcc ctttcagaac actcccgtgg
    15481 tcgaaaccat gcagtatggc gcattattag gtggtaagcg cctgcgacct ttcctggttt
    15541 atgccaccgg tcatatgttc ggcgttagca caaacacgct ggacgcaccc gctgccgccg
    15601 ttgagtgtat ccacgcttac tcattaattc atgatgattt accggcgatg gatgatgacg
    15661 atctgcgtcg cggtttgccg acctgccatg tgaagtttgg cgaagcgaac gcgattctcg
    15721 ctggcgatgc tttacaaacg ctggcgttct cgattttaag cgatgctgat atgccggaag
    15781 tgtcggatcg cgacagaatt tcgatgattt ctgaactggc gagcgccagc ggtattgccg
    15841 gaatgtgcgg tggtcaggca ttagatttag acgcggaagg caaacacgta cctctggagg
    15901 cgcttgagcg tattcatcgt cataaaaccg gcgcattgat tcgcgccgcc gttcgccttg
    15961 gtgcattaag cgccggagat aaaggacgtc gtgctctgtc tgtactcgac aagtacgcag
    16021 agagcatcgg ccttgccttc caggttcagg atgacatcct ggatgtggtg ggagatactg
    16081 caacgttggg aaaacgccag ggtgccgacc agcaacttgg taaaagtacc taccctgcac
    16141 ttctgggtct tgagcaagcc cggaagaaag cccgggatct gatcgacgat gcccgtcagt
    16201 cgctgaaaca actggctgaa cagtcactcg atacctcggc actggaagcg ctagcggact
    16261 acatcatcca gcgtaataaa taaacaataa gtattaatag gcccctgatg agttttgata
    16321 ttgccaaata cccgaccctg gcactggtcg actccaccca ggagttacgt ctgttgccga
    16381 aagagagttt accgaaactc tgcgacgaac tgcgccgcta tttactcgac agcgtgagcc
    16441 gttccagcgg gcacttcgcc tccgggctgg gcacggtcga actgaccgtg gcgctgcact
    16501 atgtctacaa taccccgttt gaccagttaa tctgggatgt ggggcatcag gcttatccgc
    16561 ataaaatttt gaccgggcgc cgcgacaaaa tcggcaccat ccgtcagaaa ggtggcctgc
    16621 acccgttccc gtggcgcggc gaaagcgaat atgacgtatt aagcgtcggg cattcatcaa
    16681 cctccatcag tgccggaatt gggatcgcag ttgctgccga gaaagaaggc aaaaatcgcc
    16741 gcaccgtctg tgtcattggc gatggcgcga ttactgctgg catggcgttt gaagcgatga
    16801 atcacgcggg cgatatccgt cctgatatgc tggtggttct caacgacaat gaaatgtcga
    16861 tttccgaaaa tgttggcgcg ctcaacaacc atctggcgca gctgctttcc ggtaagcttt
    16921 actcttcact gcgcgaaggc ggaaaaaaag ttttctctgg cgtgccaccc attaaagagc
    16981 tgctcaaacg taccgaagaa catattaaag gcatggtagt gcctggcacg ttgtttgaag
    17041 agctgggctt taactacatc ggcccggtgg acggtcacga tgtgctgggg cttatcacca
    17101 cgcttaagaa catgcgcgac ctgaaaggcc cgcagttcct gcatatcatg accaaaaaag
    17161 gtcgtggtta tgaaccggca gaaaaagacc caatcacctt ccacgccgtg cctaaatttg
    17221 atccctccag cggttgtctg ccgaaaagta gcggcggttt gccaagctat tcaaaaatct
    17281 ttggcgactg gttgtgcgaa accgcagcga aagataacaa gctgatggcg attactccgg
    17341 cgatgcgtga aggttccggc atggtcgagt tttcacgtaa attcccggat cgctacttcg
    17401 acgtggcaat tgccgagcaa cacgcagtga cctttgctgc gggtctggcg attggaggct
    17461 acaaacccat tgttgcgatc tactccacct tcctgcaacg cgcctatgat caggtgctgc
    17521 atgacgtggc gattcaaaag cttccggtcc tgttcgccat cgaccgtgcg ggcattgttg
    17581 gtgctgacgg tcaaacccac cagggcgctt ttgatctctc ttacctgcgc tgcataccgg
    17641 aaattgtcat tatgaccccg agcgatgaaa acgaatgtcg ccagatgctc tataccggct
    17701 atcactataa cgatggcccg tccgcggtgc gctacccgcg cggcaacgca gttggcgtgg
    17761 aactgacgcc actggaaaaa ctgccaattg gcaaaggcat tgtgaagcgt cgtggtgaga
    17821 aactggcgat ccttaacttt ggtacgctga tgccagaagc ggcgaaagtc gctgaatcgc
    17881 tgaacgctac gctggtcgat atgcgtttcg tgaaaccgct tgatgaagcg ttaattctgg
    17941 aaatggccgc cagccatgaa gcgctggtta ccgtagaaga aaacgccatt atgggtggtg
    18001 caggcagcgg cgtgaacgaa gtgctgatgg cccatcgtaa accagtaccc gtgctgaaca
    18061 ttggtctgcc ggacttcttt attccacaag gaactcagga agaaatgcgc gccgaactcg
    18121 gcctcgatgc caccggtatg gaagccaaaa tcaaagcctg gctggcataa tccctactct
    18181 actcctgcta tgcttaagaa attattcatg gcaggagtgg cagcatgcaa tacaacccct
    18241 taggaaaaac cgaccttcgt gtttcccgac tttgcctcgg ctgtatgacc tttggcgagc
    18301 cagatcgcgg taatcacgca tggacactgc cggaagagag cagccgtccc atcattaaac
    18361 gcgcactgga aggcggcata aatttctttg ataccgccaa tagctattcc gatggcagca
    18421 gcgaagagat cgtcggtcgc gcactgcggg atttcgcccg tcgtgaagac gtggtcgttg
    18481 cgaccaaagt gttccatcgc gttggtgatt taccggaagg attatcccgt gcacaaattt
    18541 tgcgctctat cgacgacagc ctgctccgtc tcggcatgga ttatgtcgat atcctgcaaa
    18601 ttcatcgctg ggattacaac acgccgatcg aagagacgct ggaagccctg aacgacgtgg
    18661 taaaagccgg gaaagcacgt tatatcggcg cgtcatccat gcacgcttcg cagtttgctc
    18721 aggcactgga actacaaaaa cagcacggct gggcgcagtt tgtcagtatg caggatcact
    18781 acaatctgat ttatcgtgaa gaagagcgcg agatgctgcc gctgtgttat caggaaggcg
    18841 tggcggtgat tccatggagt ccgctggcgc ggggccgtct gacgcgtcct tggggagaaa
    18901 ccaccgcacg gctggtgtcg gatgaagtgg ggaaaaatct ctaccaagaa agcaatgaaa
    18961 atgacgcaca aattgcagaa cgcttaacgg gcgtcagtga agaacttggt gcaacgcgag
    19021 cacaagttgc gctggcctgg ttgttgagta aaccgggcat tgccgcaccg attatcggaa
    19081 cttctcggga ggaacagctt gatgaactgc taaacgcggt ggatatcacc ttgaagccgg
    19141 aacagatcgc cgagctggaa acgccgtata aaccgcatcc ggtcgtagga tttaaatagg
    19201 tatgcaggcc tgatgagacg tgacaagcgt cacatcaggc atcggtgcac aactacgaca
    19261 gaatacccag cggccagtga tgaccgataa aatacaggat gccagcggag atcaccccgg
    19321 cgacaatatc gtcaatcatg atccccatgc cgccatgtac attgtgatca aaccagcgga
    19381 tcggccacgg cttccacata tccagaatac ggaaaatcac aaatccggcg gtaacccact
    19441 gccagtcatt ggtcggcagc gccatgagcg taatccacat accaataaat tcgtcccaga
    19501 caatactgcc gtgatcgtgc acgcccatgt ctttcgccgt ttgatgacaa agatagacgc
    19561 cgatacagat ccccagcatt accaccagcg aatagagctg ccagggcaaa aaagtcatca
    19621 gataccagaa gggaatcgcc gccagcgacc ccatcgtgcc aggaacgatc gggcttaatc
    19681 cacttccgaa tccgacagca agcagatgcc acggattact cattttcagg cgacttttcg
    19741 cgacatcttt atggcgtggc aaaatggtca tatcctttcc agtctaacgt gacaggttcg
    19801 ccgtcacgaa taaaacaaag cccttcgata tcggcggtca tttgcccgat acaggtaaac
    19861 ggtacgccca ggtgtccgag agccacatcc agcgcgccac ggttcagttc cggcaccgtg
    19921 aaacacaact cgtaatcttc accaccagag agcgcccaac gcagcgcctg ttccggttca
    19981 acatggcgag aaagcgcatc agaaaacggc agcaatgcca ggtcaatacg tgcgccgcag
    20041 tcgctggctt tcacgatatg cccgagatcg gaaatcagac cgtcagagag atcgatagct
    20101 gaatttgcca gatcgcgcag tgcctgcccc tgtaaaatac gcggcgacgg acggagatga
    20161 cgtttgatca agtagtccgc atctttagca tcggcaacct gcaaacggtt ttgcaaaatc
    20221 gccagcccgg cagcgctatc gcccggtgta ccggtcacat agatccagtc acccggtttc
    20281 gccccagagc gcgttaaggc tcgtcccatc ggaacaaagc cgtggatacc caacgtcatt
    20341 gataatggcc cacgcgtggt atcgccgcca atgagttgca tatcgtaata attgagaaga
    20401 tcaaacaagc tgtcgctgaa ggactcaagc cacgcttcgt ctacgtccgg taaggttaat
    20461 gccagcgtca gccaggccgg atcggccccc atcgctgcca gatcgcttag gttcaccgcc
    20521 agtgctttat aagccagatc agcaggatcg atatcaggga ggaagtggtt gcccgccacc
    20581 agcgtatcag tgctgatcgc cagggtctgt ttctcgggga tattgagaag agcgcaatcg
    20641 tcgccgatgc ccagttcgac atcaagacga gaacttctta cacggtcaaa ataacgggca
    20701 atcagggaga actcgccaca tgccatacgt tatgcctcag caggtaaaaa agaaaaggcc
    20761 ggcgatcatg gaacggtctt ccgtgaatct accggcctgg atattacttt ttgttagggc
    20821 gaatcacagg tgctgcttta tcgagtacgc cgttgacgaa cttatggctg tcttctgcgc
    20881 cgaacgattt cgccagttcg atcgcttcgt taatggccac tttgtatggc acatcgctac
    20941 gtttagacag ctcgtacagc gcaatgcgca gtactgcttt ttctacctgg cccagttctt
    21001 ccagcagacg ggacaggtac ggcttcatca gtccgtcgag gtatgcggtg ttagtcgcca
    21061 ccccggccag cagctcacgg aagtacagga cgtcaacgtc ttttacatcc tgttcagcca
    21121 ggaactggta ttcaacatca gcgatgtcgt tctgggacaa ctgccaggag tagagcgcct
    21181 ggacggcaca ctcacgagcg cggcgacgag cagcaggttt cacggatttc cccttactaa
    21241 tttcaggcct tgatggcttt caatacatta atcatttcaa gcgcggtcag tgcagcttct
    21301 gcacctttgt tgccagcttt ggtgccagca cgttcgatcg cttgttcaat gctttcagtg
    21361 gtcagaaccc caaaagcaac cggaatttcg ctgtcctggg caacatgcgc caggccgttg
    21421 cttgcaccac cagcgacata ttcaaagtgg gcagtgccac cacgaataac cgtacccagc
    21481 gcaatcaccg cgtcgtattt accggtttta gccagtgcac ccgccgccag cggcagctca
    21541 taggcacctg gcacccaaac aacggtaatg ttttcatctt ttacctgacc gatacgtttc
    21601 agtgcgtcaa ttgcaccttc cagcaggctg tcattgataa agttgttgaa acgcgcgatg
    21661 gtgatggcga cgcgagcgtc cggggtagca acgttagctt caataatgtt cattttcttc
    21721 cttcgggttc gagtatggcc ccgcaggggg gcggatttta gcataatatt tcgtgcgctg
    21781 cttccctttc gagccgggag atcatgcacc cactaaatgc aggcaaacat ccgggcctac
    21841 atgacgtatc tctttgaatt taaattgggg agcgtcggct aatttctcaa gccctggcag
    21901 cgtgcataat ccacgggcgt cgttgcctaa tagtttaggt gcgatataga caatcagctc
    21961 atcgactaaa cccgcctgta gcaatgcgcc agcgagcgtt ggccccgctt ccacccagat
    22021 gctgttaatc tgctgtttac ccagttgcat cattagtaca accagatcca ggtgaccttt
    22081 atgctctgga atcagcaagg tacgcaccgt ttccggccac tcacgagaat cttcctgcgt
    22141 acgcgcgaac caggtttcgc cgggctgctg cacaatgcga tgttccggcg tcacgcgatt
    22201 ttggctatca atcacaatac gtaccggctg gcggagattt tgttgcgggt agagtccctg
    22261 agtttgttca tccagttcag accaacgcac tgttaaggct ggatcatccg ccagcaccgt
    22321 ggcgctgctg gttaaaatgg catgactttg cgcgcgcagt cgttgtacgt cgcgcctcgc
    22381 ctgaggtgaa gtgatccact ggctttcgcc actcgccatc gccgtgcgac catcaagcga
    22441 tgcgccaagt tttaactgaa tataaggaaa gccggtgcgc atccgtttga gaaagccttt
    22501 attcaattgc tcagcttcac tcatcatcag cccgtggctg acgtcaatgc cagcctgttg
    22561 cagacggtaa agtccacgtc cagcgacctg cggattgggg tcttgcattg cagcaaccac
    22621 gcgcgccacg cccgcagcaa ttaatgcatc acaacacggt ggcgtacgac catgatggct
    22681 acagggttcg agtgtgacat acgccgttgc gcctttggct ttttcgccgg ccatgcgcaa
    22741 cgcgtgtacc tcggcatgcg gttcacccgc gcgttgatgg taaccttcac cgacaatttc
    22801 gccatctttg acaatgacgc acccgacatt cgggttggga tgcgtggtaa aacgtcctcg
    22861 ttgcgccagc tttagcgccc gcgccatgta atactcgtcc tgcacggctt agtcctccag
    22921 gcgcgcgatc tcttcgccaa attctttgat atcttcgaaa ctgcggtaga cagaggcaaa
    22981 acggatatag gcgactttat cgagcttttt caattgctcc atcaccagat tgccaatcat
    23041 cttgctcggc acttcgcgct caccggtggc gcgcagctgc gattttatat gattgattgc
    23101 catttcgacg tcatcggaac tcaccggacg tttttccagc gcccgcagca ttccgctacg
    23161 taatttctct tcattaaacg gttcacgcac gtcgttgctt tttacaacac gcggcataac
    23221 cagctccgcc acttcaaagg tggtgaaacg ttcattacac accagacact gccgacggcg
    23281 gcgtacggat gaaccctcgc ccacgagacg agagtcaatt accttagtgt ccacggcgaa
    23341 acagaatggg caatgcatac ggcgtcctga ccaggtggtt aacagaaatc tattttaccc
    23401 tgaactgacg atacaacaaa ggcgcaacgc ttttgagata aaggatcgat gctttcactg
    23461 cttttaccct ctaccattag gctaattgcg acgagaaagg gacgacctat gaatactaac
    23521 gtttttcgac tgctcctgct gggaagcctg ttcagcctta gcgcctgtgt gcagcaaagt
    23581 gaagtgcgac agatgaaaca cagcgtcagc acgctgaacc aggagatgac gcaactcaat
    23641 aaagaaacgg tcaaaatcac tcagcaaaac aagctgaatg caaaatccag cagtggggtt
    23701 taccttctgc ctggagcaaa aacaccagca agactggaaa gccagatcgg tactttacgt
    23761 atgtcgctgg tgaatattac gcctgacaca gatggcacta ccctgacgct acgtattcag
    23821 ggcgaatcca atgacccttt acccgcattc agcggaaccg ttgaatatgg gcaaattcag
    23881 ggaacaatag acaactttca ggaaatcaat gtgcagaatc aattgattaa cgcacctgcc
    23941 agcgtcctcg cccccagcga tgttgatatt ccgttacagt taaagggtat gtctgtggat
    24001 cagttaggct ttgtacgtat ccacgacatt caacctgtta tgcagtaaac gtattgcggg
    24061 gcgatattgt gcgtcccgca acatctttct ccgtcatttt gttactccgc ttacatcacc
    24121 tggattgata gtaaaagttt gcaacaaggg tgaaagtcag tacaatcccc gcccgaatgt
    24181 gtgtaaacgt gaacgcaatc gattacgtaa atgatagaac tgtgaaacga aacatatttt
    24241 tgtgagcaat gatttttata ataggctcct ctgtatacga aatatttaga aacgcaattt
    24301 gcgccttttt cactcccgca agggattttc aaacagtggc atacatatga aaaaacatta
    24361 ctggcagccg gtgcggtact ggcgctctct tcgtctttta ctgtcaacgc agctgaaaac
    24421 gacaaaccgc agtatctttc cgactggtgg caccagagcg ttaacgttgt cggaagctat
    24481 cacacccgtt tcggaccgca gctccgcaac gatacctacc ttgagtacga agcattcgct
    24541 aaaaaagact ggttcgactt ctatggttat gcggatgcgc cggtattctt cggcggtaac
    24601 tccgatgcaa aaggtatctg gaaccacggt tctccgctgt ttatggaaat cgaaccgcgt
    24661 ttctccatcg acaagctgac caatactgac cttagctttg gtccgttcaa agagtggtac
    24721 ttcgcgaaca actacattta cgacatgggt cgtaataaag atggccgcca gagcacctgg
    24781 tacatgggtc tgggtaccga catcgatact ggtctgccga tgagcctgtc catgaacgtc
    24841 tatgcgaaat accagtggca gaactacggc gcagcgaacg aaaacgagtg ggacggttac
    24901 cgtttcaaag tgaagtactt tgtgccgatt accgatctgt ggggcggcca gctgagctac
    24961 atcggcttca ccaacttcga ctggggttcc gacctgggcg atgacagcgg ttatgcgaac
    25021 aacggtatta aaacacgtac caataactct attgcgtcca gccatattct ggcgctgaat
    25081 tacgatcact ggcactactc tgtcgttgcc cgttactggc ataacggtgg tcagtggaat
    25141 gacgatgcag aactgaactt cggcaacggc aacttcaacg ttcgctctac cggctggggt
    25201 ggttatctgg tagtaggtta caacttctaa ttgtgagaat gccggatttc atatccggca
    25261 ttttttatca cttcttcttg ttcatcatcg ctttcaaatc agcgaatggg ttgtacttcg
    25321 cttcaccgac atctttctgc gcatcttccc ctgcgataac ggtcgtacca tactgatccg
    25381 cttcggtgta tttcgaatgc tcatgatcgt ggcaatagag acaaagtaat tcccagttac
    25441 tgccatcttc cgggttatta gtatggtcgt gatcaatgtg gtgaacggta agttcacgca
    25501 ggttggaata aacaaactcg cgggaacagc gaccgcagac ccacggatag atttttaatg
    25561 ctttttcgcg atagccgctt tctaaccgcg catagttttt tgggatgata gccatgaaaa
    25621 agttacctgg atgaaatgta gggttacgcg cagtttccca agatgcgggg attttcgcaa
    25681 tcaccttaca tcaaaaaaaa tcccgatctt ctgaccggga ttttcaacat cagctgaaac
    25741 ttgattaagg cagaattgac ggctgatccg ccccttcttt ttccactttc tgctgcaaca
    25801 tgtgttcgcg cttcataccc agtttcagag ccagcgccga tgccacatag atggaagatg
    25861 cagtaccgat ggaaacaccg ataagcatgg tcagcgagaa gccttccagt accggaccac
    25921 cgaagaggta cagcatcagg ataaccatca aggtagtacc ggatgtgatc aaggtacggt
    25981 gcagcgtctg ggtcaaggac acgttaaaga tttcgtaagg cgtaccgcga cggatcttgc
    26041 ggaagttttc acgaatacgg tccgatacca cgatactgtc gttaagcgag taaccgataa
    26101 ctgacatcaa cgatgccaca atggtcaggt caatctcgat atggaataac gacaaaatac
    26161 ccagcgtaat gataacgtcg tgtgccagcg caataacaac ccctgccgcc agtcgccact
    26221 caaagcggaa acctacgtac acgaggatag acagcagcgc cgccatcaac gccatcgcac
    26281 cggtttgcgc aaggtctgcc cccacgctcg gaccgacgaa ctcaatacgc ttcactgccg
    26341 cattctgatt ggtggattcg ttaatcacct tcaggacctg gctgcccagc acctgaccgc
    26401 cggtttcgcc ttcagcaggc ggcatacgga ccatgatgtc gtggctgcta ccaaagtttt
    26461 gcagcatcgg ctcttcaaaa ccggcttttt gcaatgcatc acgcattacg tcaatttcag
    26521 ccggtttttc gagcgtaatt tcaataaccg taccaccggt gaaatccagc ccccagttaa
    26581 agccgcgcac gcccataata acgatagcgg cgattaacag cagaccagag atgccaaaag
    26641 cccagtagtc ccagcgcata aagtcataga ctttacggcc gtggtttagt tgttcaacag
    26701 tatattcctg tgccacatcg cactcctcag attgacagct tcttgacgcg cttgccgcca
    26761 tatagcaggt ttacgatggc acgcgtaccg acaatcgcgg taaacatcga cgtcgccaca
    26821 ccgataccgg tagtaatcgc gaacccttta attgccccgg tacccactgc gtacaggatg
    26881 atgactttaa tcagcgtggt gatgttcgca tcgaagatag aactgaatgc gccacgataa
    26941 ccttcatcaa ttgcctgttg aacagtacgt ccgttgctca actcttcttt aatacgttcg
    27001 ttgatcagta cgttcgcatc gaccgctacc gcaagggtta agacgatacc cgcgatacct
    27061 ggcatactca gcgttgcgcc tggcaacagc gacataatac cgacaatcaa gatcaagttg
    27121 gcaatcagag cactggtcgc aatcagacca aacttcttat agaagatgat catgaacaga
    27181 atagaaacca gcagaccggc aaggcaagcc tccagcccct gctcaatgtt ctgcataccc
    27241 agggttgggc caatggtacg ttcttcaaca atctgaatcg gcgcgatcaa cgcaccggca
    27301 cgcagcagca gtgaaagctg acgcgcttcg cttgggttat tgatgccagt aatacggaag
    27361 ctgttaccca gacgagactg gatgttggcg atattaatca cctcttcctg tttcaccaga
    27421 accgcacgac cgtttgcatc tttcttaccg ctgtctttgt actccacaaa cagggttgcc
    27481 atcggtttgc cgatgttgtc cttggtgaag ttagacatga tgttaccacc cgcgctatcg
    27541 agcgagatgt taacctgcgg ctggttgtat tcgtcctggc tggaagtgga gtcggtgata
    27601 tggtcaccgg tcagaattac gcgtttgtac agcacaaccg gctgaccttc gcgggtctgt
    27661 ttcacttcag agtcgcccgg tacgcgaccg gatgccgcag cggcctgatc aacgttggtg
    27721 tttaccagac ggaattccag cgttgccgtc gcacccagaa tctctttcgc acgcgctgtg
    27781 tcctgaatac ctggcagttc aacaacgata cggtcagcac cctgacgctg aaccaccggc
    27841 tccgccacgc caagttggtt tacacggtta cgcaggatat taatgttctg ctgcaccgca
    27901 tattcacgcg cttcactcag gcgagcatcg ctcattaccg cacgcagctg gttgctgccc
    27961 tggctgctaa tcaccaggtc cggatggcgc ttgctcagat acgcaatggc ttcatcacga
    28021 gctttagcat cgcggaaagt gatgctcagg ccgtagttgt tttctttacg aacggtggta
    28081 tacgggatgc ccttttcgcg caggtcgctg cgcaggctat cgatgttttg ttcctggagt
    28141 ttgccaagcg cggtgtccat atccacttcc atcaggaagt gaacgccgcc acgcaggtca
    28201 aggccgagct tcatcggctc agcgtgaata gctgccagcc agcgcggcgt tgccggggca
    28261 aggttaagcg ccacgacgta tttgtcaccc ataacgccca ttaatgcttc acgagcgcgc
    28321 agctgggtgt cagtggagtc gaagcgcgca agaatagcgc cctcttccag tgccacagac
    28381 ttagcagtta ttttttcttc ttgtaacgtt ttctggacct ggatcagcgt ttgctcactg
    28441 gcggcgactc cgcgcgcacc agtgatctga acagccggat cctcaccaaa caggttggga
    28501 agcgcataca gcagaccgat gacaatcacc acgatcagca tgacgtactt ccacaaagga
    28561 taacggttta acacggcaat tcccttaggg aaaaatttta attacagcgc cttcatggtg
    28621 cctttcggca gaacggcagc tacgaagtca cgtttaataa ctacttcagt ggtgtcattc
    28681 agcgcgatag caatgtagcc gttttccgct actttggtta cgcgaccgac caggccaccg
    28741 ttcgtcagca cttcatcacc tttagcgatg gagtccatca gctttttgtg ttctttggtg
    28801 cgcttctgct gtggacgcag gatcatgaaa tagaaaatca gaccgaatac caccagcatc
    28861 aaaatcaaag acatcgggct accttgcgcc ggtgcacccg ttgccgctac cgcatcagaa
    28921 ataaaaaagc tcattaaatt tccctcatta ttaatattaa tcaacgttca aaggtggtac
    28981 ttctcgcccc tgacgctggt aaaaatcagt tacgaagctc tctaatttac cctcttcaat
    29041 agccttgcgt aaacccgcca tcaaacgctg gtagtaacga aggttatgaa tggtattgag
    29101 tcgcgcgcct aatatttcgt tgcaacggtc gagatgatgc aagtaagcgc gtgaataatt
    29161 gcgacaggtg tagcaatcac actcaggatc gagtgggcca gtatcgctct tatacttcgc
    29221 attgcggatt ttcaccacgc catcggtcac gaacaaatga ccatttcggg cgttgcgggt
    29281 tggcattacg cagtcaaaca tatcgatacc gcgacgcacg ccttcaacca ggtcttctgg
    29341 tttaccaacg cccatcaggt aacgcggttt gtctgccgga atttgcgggc atacatgctc
    29401 cagaatgcgg tgcatatctg ctttcggctc acccacagcc agaccgccga cagcgtagcc
    29461 atcaaaaccg atatctacca gacctttaac agaaatatca cgtaaatctt cgtaaacgct
    29521 gccctgaatg ataccaaaca gtgcattttt gtttccgaga ctgtcaaaac gctcacggct
    29581 acgcttcgcc caacgcagag acatctccat ggagcgtttt gcgtaatccc agtcagcagg
    29641 atacggcgta cactcatcaa agatcatgac gatatccgaa ccaagatcgt actgaatctc
    29701 catcgacttt tccgggtcga ggaaaatcgg gtcgccgttg atcgggttac ggaagtgcac
    29761 gccctgttcg gtgattttac gaatatcacc aaggctgaag acctggaagc cgccggagtc
    29821 ggtaagaatc ggccctttcc actgcataaa atcgtgtaaa tcgccatgca gtttcatgat
    29881 ttcctggccc gggcgcagcc acaggtggaa ggtgttaccg agaataattt gtgcgccagt
    29941 ggcttcaact tcttccggcg tcatcccttt tacggtgccg taagtgccaa caggcataaa
    30001 acaaggcgtt tccactacgc cacgatcaaa gaccaggcgg ccacggcgtg cgcgaccgtc
    30061 ggtggtgtcc agttcaaatt tcattttttc tccactacgt cagaaaaaca gtccaacgtt
    30121 taaaccagcg ccgcggaatt actccccaac gcgctcatta attgcctgcg gattgtacgt
    30181 gataaacatc gcatcaccgt aactaaaaaa gcgatatttc tcttctaccg ccgctttata
    30241 ggcattcatg gtgtgttgat aaccagcaaa tgccgaaacc agcataatca gcgtcgattc
    30301 cggaaggtgg aagttcgtca ccagcgcatc gaccactttg tactggaaac ccggatagat
    30361 aaagatttgg gtatcgtcaa agaacggttc aatgagatcg tttttcgctg cctgagccgc
    30421 actttccagc gaacgtactg aggtggtacc aaccgcaatc acccggttac cgcgcgcttt
    30481 cgccgccagt accgcgtcta ccacatcctg cggcacttca gcgtattccg agtgcatgat
    30541 gtgatcttca atggtgtcga cgcgcaccgg ctggaaggtg cccgcaccaa cgtgcaacgt
    30601 cacaaacgcc atctccacgc ctttggcgcg taatttttcc agcaaaggtt cgtcaaagtg
    30661 cagtccggcg gtcggtgctg ctaccgcgcc gggtttttcg ctataaaccg tttgataaag
    30721 ttcgcggtct gcatcttcgt ccggacggtc gatgtacggc ggcagcggca tatggccgat
    30781 gctgttgaga atatccagca ccgaacgttg atcattaaat tcgacttcaa acagtgcgcc
    30841 gtggcgcgcg gtcattgttg cgttaatacg ttcgtcatcg cccagcagca gttctgcgcc
    30901 aggttttggc gctttcgagg cgcgaatatg cgcaagaatg cgtttgtcgt cgagcatccg
    30961 ctcaaccagc acttcaatct tgccgccgct ggctttacgc ccaaacagac gcgccgggat
    31021 cacgcgggta ttattaaaaa ccagaagatc gccggggttg agcttatcga gtaaatcggt
    31081 gaaagtaccg tgcgtcagcg cgcccgtcgg cccgtccagc gacagtaaac gacagctact
    31141 gcgttcaggc atgggatagt gggcaatcag ggattcgggc aattcaaaag agaaatcggt
    31201 aacgcgcatg acactgactc agactaaaat aagaggcggg tagtctagtg ccggggcgct
    31261 ctccctgcaa cctttacccc cctttggata gaatataatc gatgaatttt ttagctcacc
    31321 tgcatttagc ccatctcgcg gaaagctcgc tttccggcaa tttactggct gatttcgtac
    31381 gcggaaatcc tgaagaaagt tttccgcccg acgtcgtggc tggcattcat atgcatcggc
    31441 gaatcgacgt attaaccgac aatctgccag aagtccgcga agcgcgggag tggtttcgta
    31501 atgaaacgcg ccgtgtcgcg cctattacgc tggatgtgat gtgggatcac tttctgtccc
    31561 gccactggtc gcagctgtcg cccgattttc cgctacagga atttacctgt tatgcccggg
    31621 agcaagtgat gactattctt ccagactcac cgccacgctt tatcaatctg aacaactact
    31681 tatggtcaga gcagtggttg gtgcgctatc gcgatatgga tttcatccag agcgtgttaa
    31741 acggcatggc aagccgtcgc ccacgtctgg atgccctgcg taactcctgg tacgatttag
    31801 acgcccatta cgatgctctc gaaacctgct tctggcagtt ttatccgcgg atgatggagc
    31861 aggcgtcacg caaggcgtta taagacgtta attcatccac accgtagccg aaatggcagg
    31921 caaagccaga atcccgtctg tcagttgccc gtgaccttct ttgcgttgcc attgcgcgac
    31981 attaagcaac ggcgacgcgg gtagcaccac ttcacaggct tcgccacggt tgattgccac
    32041 cagtacacgc tgctgataca gcacgcggac aaataccacc acgttatctt ccgcatacag
    32101 tacctgacag ccgccacgac gcagcgcctg acttttctta cgcagcgcaa tcattcgctg
    32161 gtacagcgcg aataacgccg tatcctgctt ttccacctgc caggggaacg gtttacggca
    32221 aaacggatcg tttttaccat ccagtcccac ttcatcaccg taataaatgc acggtacacc
    32281 aggccaggtg aacaaccaga ccaccgccag cggcaggcgc gcaatatccc gaccgagcag
    32341 cgttttaaat cgcgcagtat cgtggctatc gagctgatta aacatacgca gttgctgttg
    32401 atgagaaagt ccggcgcggt aattatccat ccaggccata caggtttggg cgtcaatttg
    32461 ctgcggatcg taagagatgt cggtattggc aagaaatccc cacaacggga atgtgaagcc
    32521 gcgatagttc atggcggcat cttccacatc ggcctgtaac cattgccgtg catcgccaaa
    32581 atgctctcca acgatatacg cttccggctg ggtttctttc gccgcttcgg tgatcccggc
    32641 tacgtgctgc aaattattgc gcgccccacc cgcttccccc agcatatgca ccacatccag
    32701 ccgccagccg tccatattcc acggcgcttt cagccagtgg cggacgatac tgtcttcccc
    32761 acgataaatt tcattcacca gactttccga ctgataatcc agtttcggca agctggcata
    32821 gcccagccag tcgagcgccg tgccatcgtc actaaacgag taccagtcgc gccagggcga
    32881 ttcggggttg tgacaagcac cacccgtccc gcgattgtgt ctgtcaaacc aggcatggga
    32941 atcgccactg tggttaaaca cgccgtccag caccagccgc attcccagct gctgcgtatt
    33001 gtgtcgcaaa cgcaacaacg ccccatcacc gccaaactgc ggatcgacat ggcgataatc
    33061 ctcggtatcg tatttatgta cgctgggagc tttaaacacc ggattgagat acagcgccgt
    33121 cacgccaagt tttttcagat acggcagttt ttcgcttatc ccgtctagat cgccgccata
    33181 gaacgttgat ccgcccgcct gcgccgtgac cggttcatcc cagtcacgca agatgatctc
    33241 ttgtccggct gcgtgatggt aatagacatg atcctgttca gcttcacgag gaagactacg
    33301 ggcgaaacga tcagggaaga tctgataaaa aatctgatcc gcagcccatt gtgggccgat
    33361 atccggtaca tcgacggcaa actgctccag tcgtgccggc ggcattcggc tgaagccctg
    33421 cggtgtaaac caacgctggc gatcgtgcca cagtaatttg aaactataac gccgccgagg
    33481 tagtccgccg gagagatcga tcgccgcccg ccatgcggta acaccaggct gcggctgact
    33541 gcgctgctta tgcatcggta ctgacgtttc ttcgttgtcg tgttctgtac gcagcataat
    33601 gcgctgcggt gggtcttcgc ccgtcagcca cagtgtaatg agcagttgat ctttgctttg
    33661 tttaacaaat gggggcaccg gcaggtgcca tgcatttaac atcataattc ccctgtgatg
    33721 aaaatctgat caccttgcca tagcctaatg caggatcact catcatctga aggatatttg
    33781 ggggaggagg acgggggcgg ataactttcg gaatagccgg atcaggattt cctcctgaac
    33841 cggcttacag aacgcaagga ttagtggttt tcagccagct gacgatcgtg gcggcgctta
    33901 aacatccagc caatcaacag cacaacaatc cacgcgaaac cgacatacag cgagatacga
    33961 gtatccgggt gataaccaat caacccgata ataaagagca ggaaaatcaa accgccgatg
    34021 gtggttgcta ccccacccgg tactttaaat ttcagcgact taacttcttc tggcggtaaa
    34081 cggcgacgga aggcgatttg cgacagcagg atcataatcc acacccacac cgtggcgaag
    34141 gttgccagcg aagcgatcac caggaagacg ttttccggca tgatgtagtt cagatacaca
    34201 gcaaacagca gcgcggtagt catcaccagc accgtaaccc acggaatacc gcgacgcgag
    34261 gttttgctga aaatttttgg tgcgctgccc tgctctgcca taccgtggag catacggcct
    34321 acgccaaata cgtcactgtt aattgccgac agcgaagcgg tcagcacaac aaagttaaga
    34381 atgctggcgg caaaggtaat gcccatatgc tggaacgtca gtacgaacgg gctaccggca
    34441 gtgccaacct gattccacgg gtagatagac ataatgacga acagcgtacc gacgtagaac
    34501 accagaatac gcatcggcac ggagttaatc gcacgcggaa tcgatttctc aggatctttc
    34561 gcttcaccgg cggtaatccc gataatttcg atcccaccgt aagcaaacat caccatttgc
    34621 aacgacatca ccatcccaag ccagccgtta ctgaagaagc cgccgttgct ccacaggtta
    34681 tgaataccgg tcggttgccc gccgttgcca atcccccaga tgatgatgcc gaaaccggcg
    34741 acaatcatga tgatgatggt ggcgacttta aagaacgaga accagaactc cagctcaccg
    34801 aataccttca cgctcatcag gtttacggcg caaatgatca gcaccacgct cagtacccaa
    34861 atccagtgcg gcaccgtcgg gaaccagaca cccatataga taccaaaagc ggtcacatcg
    34921 gcgatggcga caataaggat ttcaaagcag taggtccagc cggtaatgta accagccagc
    34981 gggccgaggt tttcctgcgc ataacgcgag aaagagctgg cggccgggtt atgtaccgac
    35041 atttccccca gcgcgcgcat aatgatatac gccgcgacac caccgataat ataggccaac
    35101 aacacgctcg gaccggccat tttgatggcg tctgccgaac cgtaaaacaa cccggtgcca
    35161 attgctgaac ccagtgccat aaagcgtatg tggcgggtac ttagcccacg ctttagcttg
    35221 ttcttacttt ccatcaattc ctaacccatc aacacaataa aaaaccacgg ggcatcgcag
    35281 ccccgtggtt aaaacaaatg ttcagtgatt tagtgagcgc tggaggtcac ctgacgacct
    35341 gccgcgcgat cccagataat ggccagaacc accatcacca ctgttggcat taaccacgcc
    35401 agaccttgtt cggccagcgg taaacgctgc gcccaggacg gtaagatatc gctgaatgca
    35461 gatgctttga tcccgtcgag aataccaaaa agcaggctga taaacatcgg cggagcaatc
    35521 acgcgggacg aattatgcca ccatgagcgt gtaaaactta atacaaccag tgcgatacac
    35581 ggcggataaa tggcggtcag caccggtaca gagatctgaa tcagctgact caagccgagg
    35641 ttagacacca ccatcgagaa gccgccgagg ataaacacca gcgtacgata agagagcggt
    35701 acgtactggg cgaagaattc tgcacaagca caggtcaggc caaccgccgt gaccaggcag
    35761 gcgatgaaga ttaacgccgc cagcaggaag ctaccgccgc cgccaaaggt atgctgaacg
    35821 taagcatgca ggatcgccgc accgtttgca gactgatcga ccagcgacgc gctgtctgaa
    35881 cccagacgga acagcgccag gtacagcaga gtcagaccaa cacccgccat caggccagcc
    35941 cagacggtat aacgggtcag cagacgcgct tcggtaacgc cacgagaacg cgccgcgtta
    36001 acaataacga taccaaacac cattgcgccc agcgtatcca tggtcagata gccgttgacg
    36061 aagccgttag aaaacgcagc gttttgataa gcctcagtag ccgtgctgat agaacccgcc
    36121 ggccagataa ttgcggcaac agacaggatg accagcgcga taattttcag cggcgcaagg
    36181 aagttgccca cggtatccag cagcttgccc ggatagagcg aaaccagaat aacgatagcg
    36241 aaatagacca ggctgtaaat aaacagcggc agtgcggaat cacccgtcag cggcgcaatc
    36301 cccacttcaa aagaaacggt agctgtacgc ggcgtagcga acagcggccc caccgccagg
    36361 taacacaccg ttgccagcag tacgccagca actttaccga ttggcgtgct gaggctgtca
    36421 acaccgccgc caacttttgc cagcgccacc accgttaata ccggcaggcc aacggcagta
    36481 atgaggaagc cgaatgccgc agtccagacg tgttcgcctg cctgcaagcc gaccattgga
    36541 gggaaaataa tgttacctgc gccgacgaac aacgcaaatg tcataaagcc cagagcgatg
    36601 atatcgcgcg atcttaattg atgggtcata aatcatactg cctgtggatg tggtgttgta
    36661 acgttaaatt tttgtgccaa tccctgggga tgatacagcg gtatatttaa acaattttag
    36721 cgggaaatac tcccaaccag gcgcggcgaa gaatagtttt tcgatttacc gcgttaatgc
    36781 agtcagtatg acagcacctg aagcgcaatt taaacgctta taacgtttta aagcaagaag
    36841 ggatctaaaa accagaggta tatagcgata agaaatcacc aaccagtata aaacactatt
    36901 tcaatgaaaa agacatggct aatcatgcga acaaaaaatc accaaccgtt gtaaaatcta
    36961 acgcccgtat ttcgactgca aagaaaataa gccagcatcg gctggcttat ggcaataaaa
    37021 gatgacaaag gcgaattaat cgctgttttt ggcaattaaa cgttccggga taacaaaact
    37081 gaaacgtgta acttttccta ctgtactctc aatattcagg cgactttcgt gatgattcac
    37141 cgcatgtttc acgatcgcta accctaatcc gctaccaccg gtttgccggg aacgcgcttt
    37201 atcaacgcga taaaaacgct cggtcaggcg cggaatatgc tccggtgcaa tgcccggtcc
    37261 gttatcttca acactaaatt cggcaccgtg cggcactcgc tgccagcgta cggtgatatg
    37321 cgtgccttcc ggcgtatgat tcacggcgtt atagaccagg ttcgaaatcg cactgcgtag
    37381 ctgatcttcg ttgcctgaca ccttgaggcc gttatctatc tcaaaggtaa atgtctgttt
    37441 tttctgactc agagtctgag cctcgcgctc aacgacgcgc agcatcatcg gcacatcaac
    37501 cttttcattg agcaaatgcg tcggtgcggc ttctattttc gacagcgtca gcaattgctt
    37561 caccaaccct tccatccgct gggtctgctc gcgcatggtg tgcaacgctt tttcgcgtac
    37621 cgcgccttcc agcggctgct catccatcat ctccaggtaa ccctgtaaca cggtcaatgg
    37681 cgtacgtaac tcatggctca cgttggcaaa aaagttacgc cgcgcccctt ccagttgatg
    37741 catttgcgtg acatcacgcg ccaccatcag caactgtttg tgggtataag gcataacgcg
    37801 aatttccaga tgccgcccgg tgttgagcac cagattaagc gggcgagaaa aatcacgcgt
    37861 tttcagatat tgcgtaaact ccgggtaacg cagcaggtta aggatgttct gcccgttatc
    37921 ttccggccag cgcaaaccaa gaatctgttg cgccagaccg ttacaccaga aaataccgcc
    37981 ctcttccgtg gtcagcacta ctgcgtcggg cagcgactcc gcgccactac gaaagcgttt
    38041 aatcagattg cccagttcgc ggcggcgttt tttatttcgc agctgcatct ggtgtaagcc
    38101 gtatagtagc ggttcccagc taccacgccc cggtggcggg gtcatactgc gatccaccca
    38161 cagccaccat gaaaggcgca ataaattcca gaaatgccag ataagcagtc ctgttaccga
    38221 tgccagcaaa aaccagggca ggtaaccaaa aaatgcaccc aggatgaaag ccgggaggca
    38281 gcaaagtagc agctccagca ccagcctttt ccacgacagc cgttccagca cgtaagatac
    38341 tccagttaag aaatcataag ccctgctctg cgtccgatga gcaaggcgtt aaaagcgggt
    38401 tgaaaaacga tatcctgtac cgcgcacggt ctgcaccatg cggtcatgcc cgccgggctc
    38461 cagtgcttta cgcaggcgac gaatgtggac atcgaccgtg cggtcttcca catacacgtt
    38521 agttccccag acgtggttta acagctgctc gcggctgtac acgcgctcag gatgcgtcat
    38581 aaaaaagtgc agcagtttaa attctgtcgg ccccatctcc agtggctctt cgcccgccat
    38641 cacccggtga gatgtcgggt cgaggcttag cccctgcatc tcgatcacct cttccaccgc
    38701 cattggcgaa atacggcgca ttaccgcttt gattcgcgcc accagctcct tcggcgaaaa
    38761 cggcttggtg atatagtcat ccgcgccggt ttcaaggccg cgcacgcgat cttcttcttc
    38821 ccctctggcg gtcaacatca ccactggaat atcccgggtc atcgactcgc gcttgaggtg
    38881 tttgatgaac tggataccgg agccgccagg taacatccag tcaaggagaa ttaaatccgg
    38941 ccagggttca ttcagttgat tcacagcact gtcataatct tccgcttcga ccggctgaaa
    39001 gccattttgt tcgagcacga agcagaccat ttcgcgaatt ggagcttcat cttctacgac
    39061 cagaatacgt ctcgccatga tttgccctgt tgtaataaat aggttgcgat cattaatgcg
    39121 acgtcattat gcgtcagatt tatgacagat ttatgaaaag ctcgtcgcac atatcttcag
    39181 gttattgatt tccgtggcgc agaaaaaagc aaatggcaca tctgtttggg tataatcgcg
    39241 cccatgcttt ttcgccaggg aaccgttatg cgcatccttc acacctcaga ctggcatctc
    39301 ggccagaact tctacagtaa aagccgcgaa gccgaacatc aggcttttct tgactggctg
    39361 ctggagacag cacaagccca tcaggtggat gcgattattg ttgccggtga tgttttcgat
    39421 accggctcgc cgcccagtta cgcccgcacg ttatacaacc gttttgttgt caatttacag
    39481 caaactggct gtcatctggt ggtactggca ggaaaccatg actcggtcgc cacgctgaat
    39541 gaatcgcgcg atatcatggc gttcctcaat acaaccgtgg tcgccagcgc cggacatgcg
    39601 ccgcaaatct tgcctcgtcg cgacgggaca ccaggcgcag tactgtgccc cattccgttt
    39661 ttacgcccac gtgacattat taccagccaa gcggggctta acggtattga aaaacagcag
    39721 catttgctgg cggctattac tgattattat caacaacact atgccgatgc ctgcaaactg
    39781 cgcggcgatc agcctctgcc catcatcgcc acgggacatt taacgaccgt gggtgccagt
    39841 aaaagtgatg ccgtgcgtga catctatatt ggcacgctgg acgcgtttcc ggcacaaaac
    39901 tttccaccag ccgactacat cgcgctcggg catattcacc gcgcacagat tattggcggc
    39961 atggaacatg ttcgatattg cggttccccc attccactga gttttgatga atgcggtaag
    40021 agtaaatatg tccatcttgt gacattttca aacggcaaat tagagagcgt ggaaaacctg
    40081 aacgtaccgg taacgcaacc catggcggtg ctgaaaggcg atctggcgtc gattaccgaa
    40141 cagctggaac agtggcgcga tgtatcacag gagccacctg tctggctgga tatcgaaatc
    40201 actactgatg agtatctgca tgatattcag cgcaaaatcc aggcattaac cgaatcattg
    40261 cctgtcgaag tattgctggt acgccggagt cgtgaacagc gcgagcgtgt gttagccagc
    40321 caacagcgtg aaaccctcag cgaactcagc gtcgaagagg tgttcaatcg ccgtctggca
    40381 ctggaagaac tggaggaatc gcagcagcaa cgtctgcagc atcttttcgc cacgacgttg
    40441 catagcctcg ccggagaaca cgaagcatga aaattctcag cctgcgcctg aaaaacctga
    40501 actcattaaa aggcgaatgg aagattgatt tcacccgcga gccgttcgcc agcaacgggc
    40561 tgtttgccat taccggccca accggtgcgg ggaaaaccac cctgctggac gccatttgtc
    40621 tggcgctgta tcacgaaact ccgcgccttt ctaacgtttc acaatcgcaa aatgatctca
    40681 tgacccgcga taccgccgaa tgtctggcgg aggtggagtt tgaagtgaaa ggtgaagcgt
    40741 atcgcgcgtt ctggagccag aatcgggcgc gtaaccagcc cgacggtaat ttgcaggtgc
    40801 cacgcgtaga gctggcgcgc tgcgccgatg gcaaaattct cgccgacaaa gtgaaagata
    40861 agctggaact gacggcgacg ttaaccggac tggattacgg gcgcttcacc cgttcgatgc
    40921 tgctttcgca ggggcaattt gctgccttcc tgaatgccaa acccaaagaa cgcgcggaat
    40981 tgctcgagga gttaaccggc actgaaatct acggacaaat ctcgtcgatg gtgtttgagc
    41041 agcacaaatc ggcccgcact gagctagaga agctgcaagc gcaggccagc ggcgtcgcgt
    41101 tgctcacgcc ggaacaagtg caatcgctga cagcgagttt gcaggtactt actgacgaag
    41161 aaaaacagtt acttaccgcg cagcagcaag aacaacaatc actaaactgg ttaacgcgtc
    41221 tggacgaatt gcagcaagaa gccagccgcc gtcagcaggc cttgcaacag gcgttagccg
    41281 aagaagaaaa agcgcaacct caactggcgg cgctcagtct ggcacaaccg gcacgaaatc
    41341 ttcgtccgca ctgggaacgc atcgcagaac acagcgcggc gctggcgcat actcgccagc
    41401 agattaaaga agtaaatact cgcttacaga gcacaatggc gcttcgggcg agcattcgcc
    41461 accacgcggc gaagcagtca gcagaattac agcagcagca acaaagcctg aatgcctggt
    41521 tacaggaaca cgaccgcttc cgtcagtgga acaacgaact ggcgggttgg cgtgcgcagt
    41581 tctcccaaca aaccagcgtt cgcgagcatc tgcggcaatg gcagcaacag ttaacccatg
    41641 ctgagcaaaa acttaatgcg cttacggcga tcacgttgac gttaaccgcc gatgaagttg
    41701 ctaccgccct ggcgcaacat gctgagcaac gcacgctgcg tcagcgcctg gtcgcgctgc
    41761 atggacagat tgttccccaa caaaaacgcc tggcgcaatt acaggtcgct atccagaatg
    41821 tcacgcaaga acagacgcaa cgtaacgccg cacttaacga aatgcgccag cgttataaag
    41881 aaaagacgca gcaacttgcc gatgtgaaaa ctatttgcga gcaggaagcg cgcatcaaaa
    41941 cgctggaagc tcaacgtgca cagttacagg cgggtcagcc ttgtccactt tgtggttcca
    42001 ccagccaccc ggcggtcgag gcgtatcagg cgctggagcc tggcgttaat cagtctcgat
    42061 tactggcgct ggaaaacgaa gttaaaaagc tcggtgaaga aggtgcgacg ctacgtgggc
    42121 aactggacgc cttaacaaag caacttcagc gcgatgaaaa cgaagcgcaa agcctccgac
    42181 aagatgagca agcacttact caacaatggc aagccgtcac ggccagcctc aatatcacct
    42241 tgcagccaca ggacgatatt caaccgtggc tggatgcaca agatgagcac gaacgccagc
    42301 tgcggttact cagccaacga catgaattac aagggcagat tgccgcgcat aatcagcaaa
    42361 ttatccagta tcaacagcaa attgaacaac gccagcagca gctcttaacg gcattggcgg
    42421 gttatgcact gacattgcca caggaagatg aagaagagag ctggttggcg acgcgtcagc
    42481 aagaagcgca gagctggcag catcgccaga acgaattaac cgcgctgcaa aaccgtatgc
    42541 agcagctgac gccgattctg gaaacgttgc cgcaaagtga tgaactcccg cacagcgaag
    42601 aaactgtggt gctggaaaac tggcggcagg ttcatgaaca atgtctcgca ttacacagcc
    42661 agcagcagac gttacagcaa caggacgttc tggcggcgca aagtctgcaa aaagcccagg
    42721 cgcagtttga caccgcgcta caggccagcg tctttgacga tcagcaggcg ttccttgcgg
    42781 cgctaatgga tgaacaaaca ctaacgcaac tggaacagct caagcagaat ctggaaaacc
    42841 agcgccgtca ggcgcaaacg ctggtcactc agacagcaga aacgctggca cagcatcagc
    42901 aacaccgacc tgacgggttg gctctcactg tgacgatgga gcagattcag caagagttag
    42961 cgcaaactca ccaaaagttg cgtgaaaaca ccaccagtca gggcgagatt cgccagcagc
    43021 tgaagcagga tgcagacaac cgtcagcaac aacaaacctt aatgcagcaa attgctcaaa
    43081 tgacgcagca ggttgaggac tggggatatc tgaattcgct aataggttcc aaagagggcg
    43141 ataaattccg caagtttgcc caggggctga cgctggataa tttagtccat ctcgctaatc
    43201 agcaacttac ccggctgcac gggcgctatc tgttacagcg caaagccagc gaggcgctgg
    43261 aagtcgaggt tgttgatacc tggcaggcag atgcggtacg cgatacccgt accctttccg
    43321 gcggcgaaag tttcctcgtc agtctggcgc tggcgctggc gctttcggat ctggtcagcc
    43381 ataaaacgcg tattgactcg ctgttccttg atgaaggttt tggcacgctg gatagcgaaa
    43441 cgctggatac cgcccttgat gcgctggatg ccctgaatgc cagtggcaaa accatcggtg
    43501 tgattagcca cgttgaagcg atgaaagagc gtattccggt gcagatcaaa gtgaagaaga
    43561 tcaacggcct gggctacagc aaactggaaa gtgcgtttgc agtgaaataa ctattcagca
    43621 ggataatgaa tacagagggg cgaattatct cttggccttg ctggtcgtta tcctgcaagc
    43681 tatcacttta ttggctacgg tgattggtag ccgttctggt ggttgtgatg gtggtatgaa
    43741 aaaagtcatt ttatctttgg ctctgggcac gtttggtttg gggatggccg aatttggcat
    43801 tatgggcgtg ctcacggagc tggcgcataa cgtaggaatt tcgattcctg ctgccgggca
    43861 tatgatctcg tattatgcgc tgggggtggt ggtcggtgcg ccaatcatcg cactcttttc
    43921 cagccgctac tcactcaaac atatcttatt gtttctggtg gcgttgtgcg tcattggcaa
    43981 cgccatgttc acgctctctt cgtcttacct gatgctcgcc attggtcggc tggtatccgg
    44041 ctttccgcat ggcgcatttt ttggcgtcgg cgcgatcgtg ttatcaaaaa ttatcaaacc
    44101 cggaaaagtc accgccgccg tggcggggat ggtttccggg atgacagtcg ccaatttgct
    44161 gggcattccg ctgggaacgt atttaagtca ggaatttagc tggcgctaca cctttttatt
    44221 gatcgctgtt tttaatattg cggtgatggc atcggtctat ttttgggtgc cggatattcg
    44281 cgacgaagcg aaaggaaagc tgcgcgaaca atttcacttt ttacgcagcc cggccccgtg
    44341 gttaattttc gccgccacca tgtttggcaa cgcaggtgta tttgcctggt tcagctacgt
    44401 aaagccatac atgatgttta tttccggttt ttcggaaacg gcgatgactt ttattatgat
    44461 gttagttggg ctagggatgg tgctggggaa tgtgctaagt ggccggattt caggacgtta
    44521 ttcaccactg cgcattgcgg cagtaactga ctttattatt gtgctggcac tgctgatgct
    44581 ctttttcttc ggcggcatga aaacaacgtc gcttattttt gcatttattt gttgcgcggg
    44641 attatttgcc ctttcagcac cactgcaaat attgttactg caaaatgcca aaggcggaga
    44701 gttattaggt gccgcaggtg ggcaaatagc gtttaacctc ggtagcgccg tcggcgcata
    44761 ttgcggtggt atgatgctga cgctggggct ggcatataat tacgtggcgc tgcctgccgc
    44821 cctgctttcg tttgctgcga tgtcgtcgtt gctgctgtat ggtcgctata agcgccagca
    44881 agcggcggat agtccggtgc tggcgaaacc actggggtag attactgtca ccgtgcgcgt
    44941 tgcttattgc cggatgcggc gtaaacgcct tatccggcct acgggctgct cattttgtag
    45001 gcctgataag acgcggcaag cgtcgcatca ggcatgatgc gctaattgcc tggttttttt
    45061 actcttgtgg ccataaccac gcagcgccgc gtacgccgct ggaatcaccg tgcttcgcct
    45121 tacgcaccgg cgtttcacat tcgccgccga agacaaattg tttaatcaac tgcggaaccg
    45181 tttgatataa acggtctaca ttgctcatcc cgccccccag gacaatcaca tccggatcga
    45241 gaatattcac gacatgtgcc agcgattttg ccagccgcag ctcgtagcga cgcaatgcca
    45301 gttccgctac cggatcgctt tcttcaacca ggcggataat ttcactgcct ttcagcgcat
    45361 gtccgctcaa acgacgataa tccgtcgcaa atcccgtgcc cgaaataaag gtttcaatac
    45421 aaccttgttt accgcaataa caagggactt cctcgcgata acgcagttcg tcttcgtcca
    45481 tccacggtag cggattgtgt ccccactcac ctgccgtgcc attgccgccg atatgcgccc
    45541 gcccattgaa tgccacgccc gcgccgcatc ccgtgccgat aatcacggca aataccgtct
    45601 gcgctcccgc tgccgcgcca tctactgctt ctgaaaccgc cagacagttg gcgtcatttg
    45661 ccagccgcac ttcccgctgc aacctcgcgc ttaagtcttt atcgaatggc tgaccgttga
    45721 gccaggttga attggcattc ttcaccacgc cggtgtaggg cgaaatcgaa ccaggaatgc
    45781 ccatacctac cgttccgcgc tgccctgttg cctgctccgc catatcaacc aacgtggcga
    45841 tcgtttcaat agtctgccga taatcatcac gcggcgtggg cagacgatgg cggtacaact
    45901 gctcccctgc atcgcccagt gcaatcactt cagttttggt gccgcctaaa tcgataccta
    45961 tacgcacggt actctcctta tttttttcaa tatcaatagc gtagagacgg acaactggat
    46021 tggcaatgca aggccgccga caattcgtta tcatgcccgc taaatttaac gacaaggccg
    46081 tggaaattat catgctgtgg ttcaaaaatt taatggttta ccgtcttagc cgcgagattt
    46141 cgttgcgtgc agaagagatg gaaaaacagc tagcctcgat ggcatttacc ccatgcggca
    46201 gccaggacat ggcgaagatg ggctgggttc ctccgatggg atcgcacagc gatgcgttaa
    46261 cgcacgttgc caatggtcaa attgttatct gcgcgcgcaa agaagaaaaa atcctcccgt
    46321 ctccggtgat taaacaggcg ctggaagcga aaatcgccaa actggaagcg gaacaggcgc
    46381 gtaagctgaa gaaaaccgaa aaagattcgc tgaaagacga agtgctgcac tctctgctac
    46441 cgcgtgcttt cagccgtttt agccagacaa tgatgtggat cgacacggtt aacggtttga
    46501 ttatggtgga ctgcgccagt gccaaaaaag cggaagatac gctggcactg ctgcgtaaaa
    46561 gcctggggtc gttaccggtt gtaccattga gcatggaaaa cccgattgaa ctgacgctga
    46621 ccgaatgggt tcgctccggc agcgcggcac agggcttcca gctgcttgat gaagccgagc
    46681 tgaaatcgtt gctggaagat ggcggagtga tccgcgcgaa gaaacaagat ctgaccagcg
    46741 aagagatcac caaccacatt gaagccggaa aagtggtgac taaactggcg ctcgactggc
    46801 agcagcgcat tcagtttgtg atgtgcgacg atggttcgct caagcgtctg aagttctgcg
    46861 acgagctgcg cgatcaaaac gaagatatcg accgtgaaga tttcgcccag cgctttgatg
    46921 ccgatttcat cctgatgact ggtgaactgg cagcgttaat tcaaaacctg attgaaggat
    46981 taggtggcga agcacaacgt taattgctga tttaccttta atgccggatg cgacgcctgc
    47041 cgcgtcttat ccggcgtacg aagccacacc aggaatataa tcgttagcta cagcgggcaa
    47101 taattcttag cgcagcaata ttatgcgttt tatgctgtaa cttgctccag gggcgttgtg
    47161 tcattgtttt tcctcaagcc ggtgacgcgc ttcactgcga atttttcgcg ttgccccgtt
    47221 tttcactaaa tcacgtaaca gcgaaagatc gggttgttcg acaaataaga gtaatgatgg
    47281 ctcctctttt aaccacgccg ttttcacatc ggcagccagc tgtgaattat taatagccag
    47341 cgaccgctcc tgaggctcag tcaatgtcgt taacaacacc ggagtatgtg cgttgtgcaa
    47401 tgccgtttgg cgcaaatgat cattgcgact ttgcagcaat gcacaccacc attcatcatc
    47461 tgatttatct tttaacgtcg ccagttccgc aatagtatct ggagatcttc gctgatattt
    47521 atatcaacct tgatgaatta tgaatatatc ggaaatcgaa aaaatcccag caaaggaaat
    47581 attattatgg gttaaagaga ggacatttca gaaaaatgcc ctcccgttca aggggaaggc
    47641 gaggaattac agatagcggc acagataaga ggtgggttcg gcaacttgca gatgaaactc
    47701 gctgtgaccg ggaacattaa acagcgaacc ggcttcatac acttgccagt cggtcgcgtc
    47761 aggcagtaac acattcagcg cgccactgat taccgtcatc tcttcaggct cagcggtgct
    47821 gaaggtgtat tcaccttcaa ccataacacc cacgctggcg cgaccagtgc tgctgctgga
    47881 aaagccgatc gatttcactt tgccggaaaa gtactcatta ctttgaagca taaactggcc
    47941 ctgtctggta gttgaacatc tacttcacta taggggccag aggcgctggc tgtcacgcaa
    48001 aattacatca gtaattccgc agccagccgt gcaatcaata cgttagagag caagacagga
    48061 acatcgagct gtttttgcag taaatcgcga tgacgctggt taaatcccaa acaatccagc
    48121 atgatgacat ctgcaccttt tgccagtaat tctttcccga catcaatgat tttttgttcc
    48181 gaatcatgaa tgggattacc caatgaaaat actggcggtt tctgcaaaat ttgccatttt
    48241 tgcgcctgaa cggtcagcaa ctcctcaacc ggtacgataa cccccacctg atgatcttca
    48301 acaatagagg aaaccagcgg tggcaatatc cgcgacggct caagaaagat cgtattacgc
    48361 gcggtcatac tactaatgtt tgctgtactc attaatataa tgacgtcgta accctgatta
    48421 tcgagcactt caaccacacc ttgcaggtct cgctccactt tgcgacgcga aacatgggcc
    48481 agatggttgt catttaataa tgtgagaatg gtgtcttcgc ctgcttctgg cgcgtactcc
    48541 gccatcactt cttcgcggct taacttcccc agcaggctat gatgggaaat attatcttcg
    48601 tcaatgtatt ctgtcaggag cggcaaaact tcctgcatag gtacaatgcc gatggtcagg
    48661 atcgccaacg acgcactcat tgttcttcca ccttactttt atttcgtcgt taattaccgg
    48721 gcaagtgtta agcaccactg ctgacactta cttgtgctac aaatgacaaa aagcgtagca
    48781 gcagacctgc actgcataat gtaaagattc caaatgattc cagtaatgga ttgttattgt
    48841 ttaatattct aattatttga aaaacatgaa ttgtgaaaaa tgtgacgcag atcccttgtg
    48901 gcggcactca gaatcccttc cgagcagtct gatgcggtca ctctttgtca gggtcctcat
    48961 agcgtttttt cgcatccatc gcttcctgag cggtcggatg ttcactgatc aacgagtctg
    49021 gtttaggatg atcggctctg agttggtacc aggttaccgt ctgtcctggc tttccttttt
    49081 caatcgtcac tatatatgct tcacgaggat aaggcggttt cgttggcatc gttcttcctt
    49141 atttcacggg atgaacgtta agtataggcg ctcgaaaatc aacaattgat cgtctgtgcc
    49201 agagcgctgc gaatttcaga aatcacctgg ctgggttcgt ttgttgcgtc gatgataata
    49261 tgcgcaactt cgcgatatag cgcatcgcgt tcttccagca cttcctgaac ttcttcgctc
    49321 agtggttttc ccgttaaggt tggccgtaaa tcttcttccg gtgcagcttg cagtcggtta
    49381 accaggactg atactggcgc acacaaataa accacgatcc cgttattttg catgaagtga
    49441 cgattaaatt ccgtcagaat aatgccgccg cctgtagcga taacggtgga tggcgcagtt
    49501 accgcttcca gcgccgccgt ttctctggcg cggaatcccg cccactcttc cctttcgacg
    49561 atctccgcga ccgtcatatt gagctgtgat tgcaaccact ggtcggtatc gacaaaccga
    49621 cggttaagcg aatcggcaag ggccattccg accgttgttt taccacagcc ccgaggcccg
    49681 atcagaaaaa gaggttgtgt catcgtgggt tttccccaat aggtcgcaat gcggcgaaag
    49741 ccggtgtcat gagaatagcg atcataccat caaactagta caatttcgat tgtaaagaaa
    49801 aaattccact taaagtgaaa atctcaatac atcccttact ataccaataa atattcaaga
    49861 atgaagtgta aataataaat tacatttagc cacgactacg ttgcacttcc agccaccact
    49921 tctccagctc cgcagcaaaa gcctggcggt cacgttgtga aagactatct ggtcctccgg
    49981 tctggatccc actggcacgt aaggtatcca taaaatcgcg catcgtcagg cgctcacgaa
    50041 tggtcgctgg cgtgtaacgt tcgccgcgcg gattaagcgc cgccgcgcct ttctcgatgg
    50101 cttcagcagc caaaggtata tctgcggtga tcaccaaatc gcccgcttca cactgccgaa
    50161 caatttcgtt atcagcaacg tcgaaacctg ccgcgacgcg cagcgtacga ataaatcgcg
    50221 atggcggcac gcgtaaactc tggtttgcta ccagtaccag cggcatctgc atacgttccg
    50281 ccgcgcgata caaaatctct ttaattacat tgggacacgc gtcggcatcc acccaaattg
    50341 tcataaagtc atcctttgtt gggtaatcct ctattgtgtc gcgcttttgc cttccggcat
    50401 agttctgttt atgcttctgc cagcgattat caaaacaatg aatttcacgg caggagtgag
    50461 gcaatggaaa agaaaatcgg ttttattggc tgcggcaata tgggaaaagc cattctcggc
    50521 ggtctgattg ccagcggtca ggtgcttcca gggcaaatct gggtatatac cccctccccg
    50581 gataaagtca ccgccctgca tgaccagttc ggcatcaacg ccgcagaatc ggcgcaagaa
    50641 gtagcgcaaa tcgccgacat catttttgcc gccgttaaac caggcatcat gattaaagtg
    50701 cttagcgaaa tcacctccag cctgaataaa gactctctgg tcgtttctat tgctgcaggt
    50761 atcacgctcg accagcttgc ccgcgcgctg ggccatgacc ggaaaattat ccgcgccatg
    50821 ccgaacactc ccgcgctggt taatgccggg atgacctccg taacgccaaa cgcgctggta
    50881 accccagaag ataccgctga tgtgctgaat attttccgct gctttggcga agcggaagtg
    50941 attgctgagc cgatgatcca cccggtggtc ggtgtgagcg gttcttcgcc agcctacgtc
    51001 tttatgttta tcgaagcgat ggccgacgcc gccgtgctgg gcgggatgcc acgcgcccag
    51061 gcgtataaat ttgccgctca ggcggtcatg ggttccgcaa aaatggtgct ggaaacggga
    51121 gaacatccgg gaacactgaa agatatggtc tgctcaccgg gaggcaccac cattgaggcg
    51181 gtacgcgtac tggaagagaa aggcttccgt gccgcagtga tcgaagcgat gacgaagtgt
    51241 atggaaaaat cagaaaaact cagcaaatcc tgatggcttt tgccggacgt caggccgcca
    51301 cttcggtgcg gttacgtccg gctttctttg ctttgtagag cgccaaatct gccgatttca
    51361 accactcgcg atagtgactc atttgtggat tcagcggcgc aacccccaca ctaatccgta
    51421 aagttacctg tggcgtattc ggcaagcgta acgtgtttag cccttcatgc acccgtaaca
    51481 tggcggtgat ggcgctctct gctggcgtac cggacatgat tactgcaaac tcatcgccgc
    51541 caaaccgacc aatcacatcg ctaccgcgta gggtaatttg taactgtcgg gtaagcgcca
    51601 caatcgcttc atcacccaca tcatggcccc aggtatcgtt gatgctcttg aaatggtcga
    51661 tatcgataat cagtaacgtt gcatcgcgat tatgccgccg acagttatca aattcattgc
    51721 gtaacatagt ttcccaatga cgtcggttat acacgcccgt cataccgtcg cgggtactca
    51781 tgacctgcaa cctgcgttta tgttccgcca gtttagtcgc cgtctggtag ctgacccagc
    51841 caaacagcag aggataaatg acaataatgg gaagggagag ccaccattcc agcggtgcgc
    51901 tattgaacga cacggtgatg cccgtcagct cgagggtgac aaggcaggag accaccatca
    51961 acaccagacc cgcgacaaac agacgggggc cgcctgcccc catcaaattc agacacataa
    52021 tcatcaacat cgcggtggaa ggcagtgcgt ttacgcccat tacgcctacc cacattcccg
    52081 ctaatactgc atcggttttt aagttgtaaa tttcccggct aagcggatcg acggccctgc
    52141 tcgctatttg ccaggctaaa tgcggccaga cgaacgccca gccgaccaac aacagccacc
    52201 accagcccgg cgacggatgc gaaaccagcg ttgaagcaat cggtaagaac atgccagcca
    52261 ggccaaccgc gcggggtagt ctgacgcgac gggcaaagcg cagcccggac cgctgatgtt
    52321 cgttttgagg agttaaagga ggttcctccc catgcgccgc cgcttttttg aaaaagtttt
    52381 catcattcat tatttttggg aacattcaga aacaattttc ccaaattata gagacggggc
    52441 taaaggcaga agtatgaaat ttcgggtgag ccattttcgt agctcacccg ttagcactat
    52501 caggtatcag gcggctttct tgaggcaggc gctcataaac ttattacgat catccccttt
    52561 cagagattgt tgcgttgctt gattattaca ttcgcgcatc ttttgctgct gtggcgtcaa
    52621 acttttttca ccaggcgcag acttgctgtt cttcaggcaa tcactcatgt aggtcttacg
    52681 agcatcccct ttcaacgcct gcgccgtcgc ctgctgatta caggaggtca tacgctgttg
    52741 ttgcggggtt aaggttctct cggcagcgcc gacggtggtt aaaaaaacca gtccgaaaag
    52801 caaggtaacc agtaatgtta ttttcatcgc accatccctc ttcatgtttt aaccatgagc
    52861 gtttgcgccc gtgatctgcc attaagtctg gttgctaaca gcaaaaaaac cacccgacag
    52921 cgaaaattca ccgccgggcg cggttttatt tcagccccag ggcggctttc atggtgtaga
    52981 agagatcggt ctggtcggtc agtccaacga cattggcggc atgtgggcca tacgctgcaa
    53041 tacgcaactg actgccggta tgttcttgtg aatcctcttc ggagttcccg taactcatca
    53101 ccatcactgc gccatctttg gtatttagcg cctgggtgag gcccggcgct ttggtgtccg
    53161 gcgcgacaat ctggctggcg tgggcgtgat cagcggtgac tatgaccagc gtgttgccat
    53221 cctttttagc gaattccagc gcacgttgta cggcttcatc gagatcgacc gtctcgccaa
    53281 tttgcccaca aggattcgca gcgtgatcct gtttatcgat ggatgcacct tcaacttgca
    53341 ggaaaaaacc tttctcattt ttactcaaca attcaatggc tttgtcggtc atctgcgcca
    53401 gggtcggtac actgtcatta cgttgcggat taggcgtaca ggtaactgcg ggcttatcga
    53461 tattgccgtg gtacgttgct ttcggtccta gccagcgcac tggcatattg ccgtcagcaa
    53521 acaggccaag caggggtttt tgctgattcg cttccgtcac cgcatttagc gaggcagcat
    53581 cactcaccaa ctgataacca cgcgcctgtg cctgttcacg cagcgttttt ccctgccatt
    53641 cgccgacggt tgccgtttca gcaaaggttt ttgcgccgcc gccaagcgta acatcggcac
    53701 gagcgttaag cagctgttcg gtaatcgatc cttttccgcc tttttccaga gcgttacccg
    53761 gacatttttc actggtcgcg ctcggaccgt agcatttgcg cgaggtcaca tgcgccacca
    53821 gcgcggcggg cgtggcatcc tgcaactctg cggtagaaac gttaccggtc gccagacctg
    53881 cggcttttgc catttccaga atcgttgggt gatctttttc gtgaatatcg acgcccagcg
    53941 caccgttata ggttttgaca ccagttgacc aggcggttgc tgatgcagcc gagtcggtga
    54001 cgtagtccgg tttaccggtt tttttattca gcgcatagtg agtgtattgc ccggtaagcg
    54061 gtaatgcatc gataccttta aaaaagccgc ccgcaccttc ggcataattg cgtgcagcag
    54121 taatttccga gtcccccatc ccatcgccaa tcagcaaaat aatatttttg gcgggtttat
    54181 cgctaagaga atcacgcaga gcggcggtct gatcccccgt taaacggcga gcaccgccgg
    54241 gtgcagtaat atcgccctga gcagcccggt tttccagaac aggcatttct ggtgtccggg
    54301 cttttgtcac aggggaaaac agtaacggta agagtgccag tgcaatagtg ctttgtttca
    54361 c
//
LOCUS       DAZGQL010000019        53786 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.019, whole genome shotgun sequence.
ACCESSION   DAZGQL010000019 DAZGQL010000000
VERSION     DAZGQL010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 53786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53786
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.019"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..1227)
                     /locus_tag="ACHKIR_001379"
     CDS             complement(<1..1227)
                     /locus_tag="ACHKIR_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000512755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HFO9989134.1"
                     /translation="MGIQNFDHSHHKLKIRGLKSPVDVLTFTGREQLSAPFRYDIEFT
                     STDKTIEPESVLMQDGAFSLTAPPVQGMPVQTALRTLHGVITGFKHLSSSQDEARYEV
                     RLEPRMALLTRSRQNAIYQNQTVPQIVEKILRERHQMRGQDFVFNLKSEYPSREQVMQ
                     YGEDDLTFISRLLSEVGIWFRFATDARLKIEVIEFYDDQSGYERGLTLPLRHPSGLFD
                     GETEAVWGLNTAYSVVEKSVSTRDYNYREATAEMTTGQHDATGGDNTTYGEAYHYADN
                     FLQQGDKEAAESGAFYARIRHERYLNEQAILKGQSTSSLLMPGLEIKVQGDDAPAVFR
                     KGVLITGVTTSAARDRSYELTFTAIPYSERYGYRPALIPRPVMAGTLPARVTSTVKND
                     IYAHIDKDGRYRVNLDF"
     gene            complement(1239..3884)
                     /gene="tssH"
                     /locus_tag="ACHKIR_001380"
                     /gene_synonym="clpV"
     CDS             complement(1239..3884)
                     /gene="tssH"
                     /locus_tag="ACHKIR_001380"
                     /gene_synonym="clpV"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HFO9989135.1"
                     /translation="MTGNHPAALLRRLNPYCARALDAAASLCQTRAHAEITIEHWLLK
                     LLEQGEGDITVIARRYEWDIDTLWQSLLAHLDTLPRSVRERPQLSEPLAALIRQAWLI
                     ASLEGDDPQIRSQHLLMALTEKPMLPACNDLWVLLSLSRVQLERLHPLLDAQSDECPA
                     RQPQVTEPLTSAQPESATTDVPAKTLTGKQDDALLAVLNRFTEDVTEKARSGRIDPVF
                     GRDTEIRQMVDILSRRRKNNPILVGEPGVGKTALVEGLALRIAEGNVPDSLKTVHIRT
                     LDLGLLQAGAGVKGEFEQRLKNVIDAAQKSPEPVLLFIDEAHTIIGAGNQAGGADAAN
                     LLKPALARGELRTIAATTWSEYKQYFERDAALERRFQMVKVDEPDDDTACLMLRGLKA
                     RYAQHHGVHMLDSAIQTAVRLSRRYLTGRQLPDKAVDLLDTAGARVRMSLDTLPEPLT
                     QLHARLAALDIEREAIEQDSVFYPETSPERLAELTDLRDELQAEAGHLEAQYQQEKAL
                     VQQIMTLRQEGTDSSELQQQLRTHQGFAPLLALDVDARAVATVVADWTGIPLSSLLKD
                     EQSDLLSMEQSLENRVVGQRPALCAIAQRLRAAKTGLTPENGPQGVFLLTGPSGTGKT
                     ETALTLADTLFGGEKSLITINLSEYQEPHTVSQLKGSPPGYVGYGQGGVLTEAVRKRP
                     YSVVLLDEVEKAHRDVMNLFYQVFDRGFMRDGEGREIDFRNTVILMTANLGSDHIMQL
                     LEEKPDATDADLHELLYPLLREHFQPALMARFQTVIYRPLGQEAMRTIVEMKLAQVVR
                     RLHQHYGLETEINDSLYDALTAACLLPDTGARNIDSLLNQQILPVLSQQLLAQQAAHR
                     KPAQLRLGWDEEDGIVLEFATEEMQ"
     gene            complement(4076..4567)
                     /locus_tag="ACHKIR_001381"
     CDS             complement(4076..4567)
                     /locus_tag="ACHKIR_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFO9989136.1"
                     /translation="MAIPAYLWLKDDGGADIKGSVDVQGREGSIEVVALDHDVYIPTD
                     NNTGKLTGTRTHKPFTFTKEIDASSPYLYKAVPTGQTLKTAEFKFYRINDAGQEVEYF
                     NITLDNVKLVRVAPLMHDIKDPSREKHNHLERIEFRYEKITWTYKDGNIIHSDSWNER
                     PSA"
     gene            complement(4625..6277)
                     /locus_tag="ACHKIR_001382"
     CDS             complement(4625..6277)
                     /locus_tag="ACHKIR_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFO9989137.1"
                     /translation="MRNTLKQAIVLWGMVLLLVLWWVFVSPSGVLRWAGAAVIVLAVA
                     ALLIYRRRQTWTEMTGDAGLLSLPPETYRQPVVLVCGDMSAHLFTDSPVRQVSEGLYL
                     HVPDEEQLVAQVERLLTLRPAWASQLAVAYTVMPGMYRDTAVLTGQLRRFAHSMAIVR
                     RRAGVNVPWLLWSGLSGSPLPERANSPWFICTGGEVQVATSAETTMPAQWIAQSGAQE
                     RSQRLSYLLKAESLMQWLDLNVLAELNGPEAKCPPLAMAVGLVPSLPAVDNNLWQLWI
                     TARTGLTPDIADTGTDDALPFPDALLRRLPRQSGFTPLRRACVTMLGVTTVAGIAALC
                     LSATANRQLLRQVGDDLHRFYAVPVEEFITKARHLSVLKDDATMLDGYYREGEPLRLG
                     LGLYPGERIRQPVLRTIRDWRPPEQKMEVTASLQVQTVRLDSMSLFDVGQARLKDGST
                     KVLVDALVNIRAKPGWLILVAGYTDATGDEKSNQQLSLRRAEAVRNWMLQTSDIPATC
                     FAVQGLGESQPAATNDTPLGRAVNRRVEISLVPRSDACQDVK"
     gene            complement(6290..6955)
                     /gene="tssL"
                     /locus_tag="ACHKIR_001383"
     CDS             complement(6290..6955)
                     /gene="tssL"
                     /locus_tag="ACHKIR_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssL, short
                     form"
                     /protein_id="HFO9989138.1"
                     /translation="MKKETDINIDVLLRDTFLTVVELRQGTTVRHGIELYRHCQRQVE
                     LVRERLKDAGFSRENVEHITYAQCALLDETVLSRSGMDDGQAIWMKNPLQSHFFNTLQ
                     AGELLYERMKQVLQEPAPAQAVLTCFHRVLLLGFRGRYQDPVAPERDQLISTLNGLVA
                     PFGVLPETAVLNVPLSPRQHPLLHSPFFWLVTLTLLLAGVWWGLHHWLNVLVDELLPQ
                     SLR"
     gene            complement(6952..8289)
                     /gene="tssK"
                     /locus_tag="ACHKIR_001384"
     CDS             complement(6952..8289)
                     /gene="tssK"
                     /locus_tag="ACHKIR_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HFO9989139.1"
                     /translation="MKICRPLWAEGTFLSSQQFQQQARWEAFSNDSIAQLCIRHPWGI
                     SNVLFDRDALTLGKLKTQAVRLRFADGTLIDSDVADILPPACDLRTLEGGSAIVLLAL
                     PLAHGNGGNLGQGEQAERPLRYRQEWQKVQDIYGSDSEDMAVERHALSLRFAHDNNQD
                     YITCPLARLVRDVQGNWTQDESYIPPLLTLNAHDGLVQRLDTLLLQLRAKCQRLMAMR
                     RESNQRMADFAVADVSLFWLLNALNSAEPVLSDFLRYPAVHPELVWRELARLAGALLT
                     FSLEHNVSAVPPYVHESPSTVFPPLFSLLSELLEASLPSRVIALDLASLPGNRWKADL
                     HDPRLQEEADFYLSVRSSLPAHQVLHQLPLVCKIGAPDDVTLLINVALNGVPLVPLTS
                     VPAALPLRLENQYFALDMHSDAAKSMLESGCCMIYAPGTMGDLKPELFAVLRS"
     gene            complement(8309..9847)
                     /gene="tssC"
                     /locus_tag="ACHKIR_001385"
     CDS             complement(8309..9847)
                     /gene="tssC"
                     /locus_tag="ACHKIR_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HFO9989140.1"
                     /translation="MSVQQEYATSETATLTTTESGGVYQSLFDKINLTPVSSIQEIDL
                     WQNSETLADASPDERVTAAIHVLLSCLAKSGEDVVKLDKSLLDFHIDDLDQKISKQLD
                     AVMHHPEFQKVESLWRGTWFVVQRTDFRKNVKIELLDISKEHLRQDFDDSPEIIQSGL
                     YRHTYIQEYDTPGGEPVASLISSYEFDNSPQDIALLRNISRVSAASHMPFIGSVGPKF
                     FLKNSMEEVAAIKDIGNYFDRAEYIKWKSFRDTDDSRYVGLVMPRVLGRLPYGPDTVP
                     VRSFNYVEEVKGPDHEKYLWTNASFAFAANMVKSFVNNGWCVQIRGPQAGGAVADLPI
                     HLYDLGTGNQVKIPSEVMIPETREFEFANLGFIPLSYYKNRDYACFFSANSAQKPALY
                     DTADATANSRINARLPYIFLLSRIAHYLKIIQRENIGTTKDRRVLELELNTWIRTLVT
                     EMTDPGDELQASHPLRDGKVIVEDIEDNPGFFRVRLFAVPHFQIEGMDVNLSLVSQMP
                     KAKA"
     gene            complement(9906..10406)
                     /gene="tssB"
                     /locus_tag="ACHKIR_001386"
     CDS             complement(9906..10406)
                     /gene="tssB"
                     /locus_tag="ACHKIR_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HFO9989141.1"
                     /translation="MADSFQNEVPAARVNIKLDLHTGNAKKKVELPLKLLAVGDYSNG
                     KEQRPLSERDKVDINKNNFNSVMAEFSPAVNLTVEDTLNGNGNEQNIALEFKSLKDFE
                     PEQVAKNIPQLRVLLAMRNLLRDLKSNLLDNATFRRELENILKDPTLSSELRDELAKI
                     APQENV"
     gene            complement(11038..11114)
                     /locus_tag="ACHKIR_001387"
     tRNA            complement(11038..11114)
                     /locus_tag="ACHKIR_001387"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11078..11080),aa:Met,seq:cat)
     gene            11323..12420
                     /gene="mltA"
                     /locus_tag="ACHKIR_001388"
     CDS             11323..12420
                     /gene="mltA"
                     /locus_tag="ACHKIR_001388"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HFO9989142.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGVDNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            12497..13303
                     /gene="tcdA"
                     /locus_tag="ACHKIR_001389"
     CDS             12497..13303
                     /gene="tcdA"
                     /locus_tag="ACHKIR_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HFO9989143.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMNAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(13354..13797)
                     /gene="csdE"
                     /locus_tag="ACHKIR_001390"
     CDS             complement(13354..13797)
                     /gene="csdE"
                     /locus_tag="ACHKIR_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HFO9989144.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLTQWEDKYRQLIMLGKQL
                     PALPEELKAQAKEIAGCENRVWLGYTVAENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAAAKQV"
     gene            complement(13797..15002)
                     /gene="csdA"
                     /locus_tag="ACHKIR_001391"
     CDS             complement(13797..15002)
                     /gene="csdA"
                     /locus_tag="ACHKIR_001391"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HFO9989145.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATTLKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPHLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMVHEVSFDGFTPQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            15194..15421
                     /gene="ygdI"
                     /locus_tag="ACHKIR_001392"
     CDS             15194..15421
                     /gene="ygdI"
                     /locus_tag="ACHKIR_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HFO9989146.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            15772..16689
                     /gene="gcvA"
                     /locus_tag="ACHKIR_001393"
     CDS             15772..16689
                     /gene="gcvA"
                     /locus_tag="ACHKIR_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HFO9989147.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            16708..17103
                     /gene="ygdD"
                     /locus_tag="ACHKIR_001394"
     CDS             16708..17103
                     /gene="ygdD"
                     /locus_tag="ACHKIR_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HFO9989148.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            17096..18196
                     /gene="rlmM"
                     /locus_tag="ACHKIR_001395"
     CDS             17096..18196
                     /gene="rlmM"
                     /locus_tag="ACHKIR_001395"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HFO9989149.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPADAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(18240..18971)
                     /gene="fucR"
                     /locus_tag="ACHKIR_001396"
     CDS             complement(18240..18971)
                     /gene="fucR"
                     /locus_tag="ACHKIR_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HFO9989150.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVAASLVTA"
     gene            complement(19029..19451)
                     /gene="fucU"
                     /locus_tag="ACHKIR_001397"
     CDS             complement(19029..19451)
                     /gene="fucU"
                     /locus_tag="ACHKIR_001397"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HFO9989151.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            complement(19453..20871)
                     /gene="fucK"
                     /locus_tag="ACHKIR_001398"
     CDS             complement(19453..20871)
                     /gene="fucK"
                     /locus_tag="ACHKIR_001398"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008737 - L-fuculokinase activity
                     [Evidence IEA];
                     GO_process: GO:0042355 - L-fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HFO9989152.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTDCHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIERLISAQQLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGAEQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYAGSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQILIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTTQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            complement(20980..22755)
                     /gene="fucI"
                     /locus_tag="ACHKIR_001399"
     CDS             complement(20980..22755)
                     /gene="fucI"
                     /locus_tag="ACHKIR_001399"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HFO9989153.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVRKPFVV
                     ATENDSLNGVAMLMGHQLTGTAQIFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGKPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            complement(22788..24104)
                     /gene="fucP"
                     /locus_tag="ACHKIR_001400"
     CDS             complement(22788..24104)
                     /gene="fucP"
                     /locus_tag="ACHKIR_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HFO9989154.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            24651..25298
                     /gene="fucA"
                     /locus_tag="ACHKIR_001401"
     CDS             24651..25298
                     /gene="fucA"
                     /locus_tag="ACHKIR_001401"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008738 - L-fuculose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006004 - fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HFO9989155.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRPIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EANLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            25326..26474
                     /gene="fucO"
                     /locus_tag="ACHKIR_001402"
     CDS             25326..26474
                     /gene="fucO"
                     /locus_tag="ACHKIR_001402"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFO9989156.1"
                     /translation="MANRMILNETAWFGRGAVGSLTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPTLAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(26529..27284)
                     /gene="xni"
                     /locus_tag="ACHKIR_001403"
     CDS             complement(26529..27284)
                     /gene="xni"
                     /locus_tag="ACHKIR_001403"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0017108 - 5'-flap endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0033567 - DNA replication, Okazaki fragment
                     processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HFO9989157.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSASGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVVGIGPKSATQLLVEFKSLEGIYENLNAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(27395..28762)
                     /gene="sdaB"
                     /locus_tag="ACHKIR_001404"
     CDS             complement(27395..28762)
                     /gene="sdaB"
                     /locus_tag="ACHKIR_001404"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HFO9989158.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            complement(28820..30109)
                     /gene="sdaC"
                     /locus_tag="ACHKIR_001405"
     CDS             complement(28820..30109)
                     /gene="sdaC"
                     /locus_tag="ACHKIR_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HFO9989159.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPIIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            complement(30665..32029)
                     /gene="ppnN"
                     /locus_tag="ACHKIR_001406"
     CDS             complement(30665..32029)
                     /gene="ppnN"
                     /locus_tag="ACHKIR_001406"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HFO9989160.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
     gene            complement(32141..32989)
                     /gene="queF"
                     /locus_tag="ACHKIR_001407"
     CDS             complement(32141..32989)
                     /gene="queF"
                     /locus_tag="ACHKIR_001407"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HFO9989161.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSANLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKVSVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            33057..33602
                     /gene="syd"
                     /locus_tag="ACHKIR_001408"
     CDS             33057..33602
                     /gene="syd"
                     /locus_tag="ACHKIR_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HFO9989162.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            34224..34553
                     /gene="yqcC"
                     /locus_tag="ACHKIR_001409"
     CDS             34224..34553
                     /gene="yqcC"
                     /locus_tag="ACHKIR_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HFO9989163.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDKPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLDNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            34553..35335
                     /gene="truC"
                     /locus_tag="ACHKIR_001410"
     CDS             34553..35335
                     /gene="truC"
                     /locus_tag="ACHKIR_001410"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HFO9989164.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDLIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMLVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            35353..35802
                     /gene="yqcA"
                     /locus_tag="ACHKIR_001411"
     CDS             35353..35802
                     /gene="yqcA"
                     /locus_tag="ACHKIR_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFO9989165.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDTLGFQPNLRYGVIALGDSSYVNFCN
                     SGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEHWGTMLS"
     gene            36237..37589
                     /gene="gudP"
                     /locus_tag="ACHKIR_001412"
     CDS             36237..37589
                     /gene="gudP"
                     /locus_tag="ACHKIR_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HFO9989166.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            37591..38931
                     /gene="gudX"
                     /locus_tag="ACHKIR_001413"
     CDS             37591..38931
                     /gene="gudX"
                     /locus_tag="ACHKIR_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HFO9989167.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVKAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGQALNVPVCELLGPGKQRDAITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVGLDWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            38952..40292
                     /gene="gudD"
                     /locus_tag="ACHKIR_001414"
     CDS             38952..40292
                     /gene="gudD"
                     /locus_tag="ACHKIR_001414"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008872 - glucarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HFO9989168.1"
                     /translation="MSSQFSTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDKCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PQKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(40516..43272)
                     /gene="barA"
                     /locus_tag="ACHKIR_001415"
     CDS             complement(40516..43272)
                     /gene="barA"
                     /locus_tag="ACHKIR_001415"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HFO9989169.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSLPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            43329..44630
                     /gene="rlmD"
                     /locus_tag="ACHKIR_001416"
     CDS             43329..44630
                     /gene="rlmD"
                     /locus_tag="ACHKIR_001416"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFO9989170.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKALFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHEVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVRACLGSLQAIRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVEPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            44678..46912
                     /gene="relA"
                     /locus_tag="ACHKIR_001417"
     CDS             44678..46912
                     /gene="relA"
                     /locus_tag="ACHKIR_001417"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HFO9989171.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            47008..47799
                     /gene="mazG"
                     /locus_tag="ACHKIR_001418"
     CDS             47008..47799
                     /gene="mazG"
                     /locus_tag="ACHKIR_001418"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HFO9989172.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     GSAGNSREVLARWEQIKTEERAQKAQHSALDDIPSSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQVVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            48027..49664
                     /gene="pyrG"
                     /locus_tag="ACHKIR_001419"
     CDS             48027..49664
                     /gene="pyrG"
                     /locus_tag="ACHKIR_001419"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine hydrolyzing CTP synthase"
                     /protein_id="HFO9989173.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            49752..51050
                     /gene="eno"
                     /locus_tag="ACHKIR_001420"
     CDS             49752..51050
                     /gene="eno"
                     /locus_tag="ACHKIR_001420"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFO9989174.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(51110..51979)
                     /gene="ygcG"
                     /locus_tag="ACHKIR_001421"
     CDS             complement(51110..51979)
                     /gene="ygcG"
                     /locus_tag="ACHKIR_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFO9989175.1"
                     /translation="MRYFILIFTFVCSFVAAQPTIVPQLQQQVTDLTSSLNSQEKKEL
                     TRKLESIFNNTQVQIAVLIIPTTKDETIEQYATRVFNNWRLGDAKRNDGILIIVAWSD
                     RTVRIQVGYGLEEKVTDALAGDIIRSNMIPAFKQQKLAKGLELAIIALNNQLTSQHQY
                     SANPSESESASSSDHYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSSVCISAIYLLDL
                     FLFSDKTFSIAVFSFFFTFTIFMVFTCLCVLQKRASGRSYHSDNSGSAGGSGGGFSGG
                     GGSSGGGGASGRW"
     gene            52274..52945
                     /gene="queE"
                     /locus_tag="ACHKIR_001422"
     CDS             52274..52945
                     /gene="queE"
                     /locus_tag="ACHKIR_001422"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HFO9989176.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
     repeat_region   53285..53618
                     /inference="COORDINATES: alignment:crt:1.2"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=53285..53313
                     /rpt_unit_seq="gagttccccgcgccagcggggataaaccg"
BASE COUNT        12954 a        13413 c        14343 g        13076 t
ORIGIN      
        1 aaagtcgagg ttgacccggt agcgcccgtc cttgtcgata tgggcgtaga tgtcgttctt
       61 caccgtactg gtgacccgcg ccgggagcgt tccggccatt acggggcggg ggataagcgc
      121 cggacggtag ccgtagcgct ctgagtacgg tatcgccgtg aacgtcagct catagctgcg
      181 gtcacgcgcc gcgctggtcg tcacgccggt aatcagcacg cccttgcgga acaccgcagg
      241 cgcgtcgtcg ccctgaactt ttatctccag ccccggcatc agcagtgaac tggtgctctg
      301 gcctttgaga atggcctgtt cattcaggta gcgttcgtga cggatacggg cataaaacgc
      361 gccgctttcc gctgcctctt tatcgccttg ttgcaggaag ttgtcggcat agtggtaggc
      421 ttcgccgtaa gtggtgttat cgcccccggt ggcgtcatgc tggcctgtgg tcatttccgc
      481 cgtggcttca cggtagttgt agtcccgcgt ggagacactt ttttccacca cgctgtacgc
      541 ggtgttcagc ccccatacgg cttccgtttc accatcaaac agcccagaag gatggcgaag
      601 cggcagcgtc agcccgcgct cgtagccgct ctggtcgtcg taaaattcga taacttcgat
      661 tttcaaccgg gcgtcggtgg caaagcggaa ccagataccc acttctgaca gcaggcgtga
      721 aataaacgtc aggtcgtcct caccgtactg catcacctgt tcacgggacg ggtattcgct
      781 tttgaggtta aacacgaagt cctgaccgcg catctggtgc cgctcgcgca ggattttttc
      841 gacgatttgc ggcaccgtct ggttctggta gatggcgttc tggcggctgc gggtaagcag
      901 cgccatacgg ggttcaaggc gcacttcgta acgggcttcg tcctgtgacg atgagagatg
      961 tttaaagccg gtaatgacgc cgtgcagggt gcgcagggca gtctgtaccg gcattccctg
     1021 taccggcggt gccgtcaggc tgaatgcgcc atcctgcatc agcaccgact ccggctcgat
     1081 ggttttatcg gtactggtaa attcaatgtc gtagcggaag ggtgcgctga gttgttcccg
     1141 gcccgtaaac gtcagcacgt ccaccgggct tttcaggccg cgaattttga gcttgtggtg
     1201 tgagtggtcg aaattctgaa tacccataaa agcctccgtt attgcatctc ttccgtagcg
     1261 aactccagta caatcccgtc ttcctcatcc cagccaagcc gtagttgggc tggtttacgg
     1321 tgcgcggcct gctgtgccag caactgctga cttagtaccg gcaggatttg ctggttcaac
     1381 aggctgtcga tattgcgtgc accggtgtcc ggcagcaggc aggcggcggt cagcgcgtca
     1441 tacaggctgt cgttgatttc cgtttccagc ccatagtgct gatgaagacg acgaaccacc
     1501 tgcgccagtt tcatttccac aatggtgcgc atcgcctcct gtcccagcgg gcggtaaatc
     1561 accgtctgaa aacgcgccat cagtgcaggc tggaagtgct cgcgcagcag gggatacagc
     1621 agttcatgca ggtctgcgtc cgtggcgtcc ggtttttcct ccagcagttg catgatgtgg
     1681 tcgctgccca gattagccgt catcagaatc acggtgttgc ggaagtcaat ttcccggcct
     1741 tcgccgtcgc gcataaaacc ccggtcaaat acctgataga acaggttcat tacgtcgcgg
     1801 tgcgcctttt ccacttcgtc gagcaacacc acgctgtacg ggcgtttgcg aacggcttcg
     1861 gtaagtacgc cgccctgacc gtagccaacg tagcccggag gagacccctt aagctgggag
     1921 acggtatgcg gctcctgata ttcggagagg ttgatggtga taagagattt ttcaccgcca
     1981 aacagggtgt cggccagtgt gagcgcagtt tcggttttac cggtgccgct ggggccggtc
     2041 agcaggaata ccccctgtgg gccgttctcc ggcgtgaggc cggttttagc cgcccgcaac
     2101 cgctgtgcga tggcgcagag cgccgggcgt tgcccgacaa cgcggttttc aaggctctgt
     2161 tccatactga gcaggtcgct ctgctcgtcc ttgagcaggg aagagagcgg gatgccagtc
     2221 cagtccgcca ccaccgtggc gacggcgcgg gcgtccacat ccagcgccag cagcggtgca
     2281 aagccctgat gcgtccgcag ttgctgttgc agttcagagc tgtctgttcc ttcctgacgc
     2341 aacgtcataa tctgctgcac cagtgccttt tcctgctgat actgcgcttc cagatgcccg
     2401 gcttctgcct gcagctcatc acgcaaatcg gtcagttctg ccagccgctc cgggctggtt
     2461 tcgggataaa acacgctgtc ctgctcaatc gcctcccgct cgatatccag cgccgccagt
     2521 cgcgcatgaa gctgcgtcag cggctccggc agggtgtcga ggctcatgcg gactcttgct
     2581 ccggcggtat ccagcaaatc aaccgccttg tccggcagct ggcgtccggt cagatagcgg
     2641 cgcgacaggc gcacggcggt ctgaatggcg ctgtccagca tatgcacgcc gtggtgctgt
     2701 gcataacggg ccttcagtcc ccgcagcatc agacaggcgg tgtcatcatc cggctcgtca
     2761 accttaacca tctggaagcg gcgctccagc gcggcgtcgc gctcaaaata ctgtttgtat
     2821 tcgctccacg tggtcgccgc gatggtgcgc agttcgcccc ttgccagtgc cggtttcagc
     2881 aggttcgccg catccgcgcc gccagcctgg ttacccgcgc cgataatggt atgggcttca
     2941 tcaataaaca gcagtaccgg ctccggcgat ttctgtgctg catcaatcac gtttttcagt
     3001 cgctgctcaa attcaccttt aacacccgcg ccagcctgta acagaccgag gtccagcgtg
     3061 cggatatgca ccgttttcag gctgtcgggc acgttgcctt cggcgatacg cagcgccagc
     3121 ccttccacca gtgcggtttt gcccaccccc ggttctccca ccagaatcgg gttgtttttg
     3181 cggcgacggg agaggatatc gaccatctgg cgaatttcgg tgtcgcgccc gaatactggg
     3241 tcgattcgcc cgctacgggc tttttccgtc acgtcttcgg taaagcggtt aagcaccgcc
     3301 agcagggcgt catcctgttt ccccgtcagc gtttttgccg gtacgtccgt cgttgccgac
     3361 tctggttgcg cggatgtcag cggttcggtg acctgtggct gacgtgccgg acattcatcc
     3421 gactgcgcat ccagcagggg atgcagccgc tcaagctgca cgcggctcag actcagcaat
     3481 acccacaggt cattacaggc gggcagcatc ggtttttctg tcagcgccat cagcaggtgc
     3541 tggctgcgga tttgtggatc gtcaccttcc agtgacgcta tcagccacgc ctgtctgata
     3601 agcgctgcca gcggttcaga aagttgagga cgttcgcgga ccgagcgggg taaggtgtcc
     3661 agatgcgcca gcagagactg ccagagcgtg tcgatatccc attcatagcg acgggcaatc
     3721 accgtgatat cgccttctcc ctgctccagc agtttcagca gccagtgttc aatggttatc
     3781 tccgcatggg cgcgggtctg acacagtgag gcggcagcgt ccaacgcccg tgcacagtat
     3841 gggttaaggc gacgcagcag cgcggcgggg tgatttcctg tcatagaagg gtttccttat
     3901 cgtgttctgt attcctgcga tccgcgttca ggcacgctac cgcccatagc cgttacctca
     3961 cacaggccca tcaggtttca ccagattgtg ggtatgcttt gctcagtacc ggtgcctgcg
     4021 gcactggaca aagcacacaa caaaaaggca gacagtttcc tgtctgccgc agttatcagg
     4081 cggaaggacg ctcattccac gagtcggaat gaatgatgtt gccgtctttg taagtccagg
     4141 tgattttctc gtagcggaac tcaatacgtt ccaggtggtt atgcttctct ctggaaggat
     4201 ccttgatgtc gtgcatcagc ggagcgactc tgaccagctt gacgttatca agcgtgatgt
     4261 tgaagtactc cacttcctga ccggcatcgt tgatgcggta aaacttaaat tctgccgttt
     4321 tcagggtctg accggtgggc acagctttgt agagatacgg actggacgca tcgatttctt
     4381 tggtaaacgt aaagggcttg tgagtacggg taccggtcag tttgccggtg ttgttgtcgg
     4441 tcgggatgta cacatcgtga tccaacgcca ccacttcgat gctgccttcg cgcccctgaa
     4501 cgtccacgga acctttgata tctgcgccgc cgtcatcttt cagccagaga taagcaggaa
     4561 tagccatgag aattactctc cttgttagtg tgattgaagc accgccttct tccggcggta
     4621 tgttttattt cacgtcctga caggcgtcag aacgcggaac aagactgatt tcgacgcgcc
     4681 ggttgactgc cctgcccagt ggcgtatcgt tggtcgccgc aggctggctc tcacccagtc
     4741 cctgtacggc aaaacaggtg gccgggatgt cgctggtctg cagcatccag ttacgcaccg
     4801 cttcggcacg tcgcagcgat aactgctgat tgcttttttc gtcgccggtg gcgtcggtat
     4861 atccggccac gaggatcagc cagcccggtt ttgcccggat gttcaccagt gcgtccacca
     4921 gcacttttgt cgagccgtct ttcaggcggg cctgtccgac gtcaaacagc gacatactgt
     4981 caagacgcac ggtctgaacc tgaagcgaag ccgtcacctc cattttttgt tcaggcggac
     5041 gccagtcgcg aatggtgcgt aataccggct ggcggatgcg ttcgccgggg tataacccca
     5101 gaccgaggcg cagtggttct ccttcccggt aatacccatc gagcatggtc gcatcgtctt
     5161 tcagcaccga caggtgacgg gctttggtga taaattcctc caccgggacg gcataaaacc
     5221 ggtgcagatc gtcaccgacc tgccgtaata actggcgatt tgccgtggct gacaggcaca
     5281 gcgcggcgat acccgccacg gtggtgacgc ccagcatggt cacgcaggcc cgtcgcagcg
     5341 gggtaaagcc cgactgacgc ggcaaccgcc gtaacagggc atccgggaat ggcagcgcat
     5401 cgtctgtgcc ggtgtccgcg atatccggcg tcaggccggt tctggcggtg atccacaact
     5461 gccataggtt gttatccacc gcaggcaacg aggggaccag ccccacggcc atcgccagtg
     5521 gtggacattt cgcctccggg ccgttcagtt ccgccagcac attcagatcc agccattgca
     5581 tcaggctttc ggctttcagc agataagaga gtcgctgact gcgttcctgt gcgccggatt
     5641 gtgcaatcca ctgcgcgggc atggtggtct ctgcggatgt tgctacctga acttcgccgc
     5701 cggtacagat aaaccacggt gaattcgctc tctccggcaa cggcgagccg gacagcccgc
     5761 tccagagaag ccagggtacg tttacgcccg cccggcgacg cactatcgcc atactgtgag
     5821 cgaaccgtcg cagttgtccg gtcagaaccg ccgtatcccg gtacatgccg ggcatgacgg
     5881 tatacgccac ggcaagctgc gatgcccacg ccgggcgaag ggtcagtaat cgctccacct
     5941 gcgccacaag ctgttcttca tcgggaacat gcagatacag cccttctgaa acctgccgga
     6001 ccgggctgtc agtgaacaga tgcgctgaca tatcgccaca gaccagcact accggttgtc
     6061 ggtacgtttc cggcggcagc gataacaacc cggcatcgcc ggtcatctcc gtccacgtct
     6121 gcctgcgccg gtaaatcaac aatgcggcaa ccgccagaac gataactgct gcaccggccc
     6181 atctcagtac gccagacgga ctgacaaaca cccaccacag caccagcagt aacaccattc
     6241 cccacagcac gatggcctgt ttcagcgtgt tcctcactca cggctccctt taccgcaggc
     6301 tttgcggcag caattcatcc accagcacgt tcagccagtg gtgcagtccc caccagacac
     6361 cggccagtag cagcgtcagc gttaccagcc agaaaaacgg cgaatgcagc aaaggatgct
     6421 gacgggggga taacggcacg ttcagtaccg ccgtttccgg cagcacgcca aatggcgcga
     6481 ccagcccgtt gagcgtcgaa ataagctggt cacgctccgg ggccaccgga tcctgataac
     6541 gcccgcgaaa tcccagcagc aggacacggt gaaagcaggt cagcactgcc tgcgcggggg
     6601 cgggctcctg caacacctgc ttcatccgtt cgtacagcag ctcgcctgcc tgcagagtgt
     6661 tgaagaaatg cgactggagt gggtttttca tccagatggc ctgaccgtcg tccatgccac
     6721 tacggctgag caccgtttcg tccagcaggg cgcactgcgc gtaagttatg tgctccacgt
     6781 tctcccgact gaatccggca tctttcagac gttcgcggac cagttccacc tgccgctgac
     6841 agtggcgata cagctcgata ccgtgacgca cggtggttcc ctgacgcagt tccaccaccg
     6901 tcagaaacgt gtcgcgcagc agcacgtcga tattgatatc cgtctctttt ttcatgagcg
     6961 cagcaccgca aacagttcag gtttcaggtc gcccattgtt cccggcgcat aaatcatgca
     7021 gcacccggac tccagcatcg atttagccgc gtcactgtgc atatccagcg caaaatactg
     7081 gttctccagc cgcagcggca gcgccgccgg aaccgaggtg agtggcacca gtggaacacc
     7141 gttcagcgcc acgttgataa gcagcgtgac gtcatcaggc gcaccgattt tgcagaccag
     7201 cggtagctgg tgcagtacct gatgcgcagg cagtgaggaa cgaacggaca ggtaaaaatc
     7261 ggcttcttcc tgcaggcgcg gatcgtgcag gtcggctttc cagcggttgc ccggcaggga
     7321 cgccagatcc agcgcgatca cccgcgacgg caggctggct tccagcagct cgctgagcag
     7381 ggagaacagt ggcggaaaga cggtggacgg cgattcgtgg acatagggcg gaacggcgct
     7441 gacgttatgc tcaagggaaa acgtcagcag cgcaccggca agcctcgcca gctcccgcca
     7501 gaccagttcg gggtggacag ccgggtagcg caggaaatcg ctcagcaccg gctcggcgga
     7561 attcagggcg ttaagcagcc agaacagaga gacatcggcg acggcgaagt ccgccatgcg
     7621 ctggttgctt tcccggcgca tcgccatcag gcgctggcac ttcgcccgta gctgcaacag
     7681 cagcgtgtcc aggcgctgta ccagcccgtc atgggcgttt aacgtcagta acggcggaat
     7741 atagctctca tcctgcgtcc agttgccctg aacatcccgc accagacgcg ccagcggaca
     7801 ggtgatgtaa tcctgattgt tatcatgggc aaagcgcagg gacagtgcat ggcgctcaac
     7861 ggccatatct tcactgtcac tgccgtagat atcctgcacc ttttgccatt cctgacgata
     7921 gcgcagcggc ctttcagcct gctcgccctg accgagatta ccgccgttac catgcgcaag
     7981 cggcagcgcc agcaaaacaa tcgcgctgcc gccctccagc gtgcgcaggt cacaggcagg
     8041 cggcagaata tccgccacgt ctgaatcgat cagcgtacca tcagcaaaac gcaggcgtac
     8101 tgcctgcgtt ttcagctttc cgagcgtcag ggcatcccgg tcaaagagca cgtttgagat
     8161 cccccacgga tggcgaatac aaagctgcgc aatgctgtca ttgctgaacg cttcccagcg
     8221 ggcctgctgc tgaaactgct gagaagacag gaatgtcccc tcagcccaca gtggtcgaca
     8281 aattttcata catcacacca ccgcccgatc aggcttttgc ttttggcatc tgggaaacca
     8341 gagaaaggtt gacgtccatc ccttcgatct ggaaatgcgg cacggcaaag agtctgacgc
     8401 ggaaaaagcc cggattgtcc tctatgtcct cgacgataac tttcccgtcg cgcagtggat
     8461 gcgaagcctg aagttcatcg cccggatcgg tcatttccgt caccagcgtg cggatccagg
     8521 tgttcagctc cagttccagc acgcgtctgt ctttggtggt gccgatattc tcgcgctgaa
     8581 tgattttcag gtagtgagca atgcgggaca gcaggaagat atagggcaaa cgggcgttga
     8641 tacggctgtt ggcggtggcg tcagcggtat catacagcgc cggtttctgg gcagagttcg
     8701 ctgagaagaa gcaggcgtaa tcgcgattct tataataaga gagcgggata aagccaaggt
     8761 tggcgaactc aaactcgcgg gtttccggga tcatcacttc ggacggaatt ttgacctgat
     8821 tgccggtgcc gagatcgtaa agatggatcg gcagatcggc tactgcgcca cccgcctgcg
     8881 gaccacggat ctgcacacac cagccattat tcacaaaact cttcaccata ttggcggcaa
     8941 aggcaaacga ggcgtttgtc cagaggtact tttcgtgatc cgggcctttg acctcttcca
     9001 catagttaaa gctgcgtacc ggcaccgtgt ccgggccata gggcagacgg cccagcacgc
     9061 gcggcatcac taatcccacg tagcggctgt cgtcggtatc gcggaacgac ttccatttga
     9121 tatattccgc gcggtcaaaa tagttgccga tatctttaat tgcggcgact tcttccatcg
     9181 agtttttaag gaagaacttc ggtccgacgg aaccgataaa gggcatatgt gatgcagcag
     9241 agactctgga aatattacgc agcagggcaa tatcctgcgg gctgttatcg aattcatagc
     9301 tggatattaa tgaggctaca ggttcgccac ccggcgtatc atattcctga atgtatgtat
     9361 ggcgatataa accactttga atgatttccg gagaatcatc aaagtcctgc cgcagatgtt
     9421 ctttactgat atccagcagt tcaattttga catttttgcg aaaatcggtg cgctgtacga
     9481 cgaaccatgt gccgcgccag agcgactcga ctttctggaa ttccggatgg tgcatgactg
     9541 catcaagctg tttactgatt ttctgatcca aatcgtcgat atgaaaatcc agcaggctct
     9601 tgtcgagttt aaccacgtcc tcgcctgatt tcgccagaca ggaaagcaga acgtggatcg
     9661 ccgccgtcac gcgctcatcg ggtgaagcat cggccagcgt ttcgctgttt tgccataaat
     9721 cgatttcctg aatggaagag accggggtta aattaatttt atcaaacagg gactgataaa
     9781 cgccgccgga ctcagtggtg gtgagtgttg cagtttcaga ggtggcatat tcttgttgta
     9841 cagacatcag catttccttt ttagggatcc tttcttctcc ggtttaacct gaatacgcgt
     9901 tcgggttaaa cgttctcctg cggcgcaatt ttcgccagtt catcacgcag ttctgaactg
     9961 agcgtcgggt ctttcaggat attttccagt tcacggcgaa acgttgcgtt atccagcaga
    10021 ttggatttca ggtcacgtag taaattgcgc atagcgagta atacacgaag ttgcggaata
    10081 ttttttgcta cctgttcggg ttcaaaatcc ttcaggcttt taaattcaag cgcgatattt
    10141 tgttcattac cgtttccgtt tagcgtatct tctactgtta aattaaccgc aggcgaaaac
    10201 tcagccatga cgctgttgaa gttgttttta ttgatatcaa ccttgtcccg ttcggacagc
    10261 ggacgttgct cttttccgtt actgtaatcg cctacggcaa gaagcttgag ggggagttca
    10321 actttctttt tagcattgcc tgtatgcaga tcaagcttga tatttacacg agcggcggga
    10381 acttcattct ggaaactgtc ggccatatcc ttttcctcat catccacttt ttctgatggt
    10441 tattgacata attatattca ggggattatc ggatgattta aataaaaagc aatgatgagg
    10501 aaaatataca aatgaataat gtatttttca ggggaataga tacagttgta tcattttaga
    10561 tattgtttgg cctcttgatt atttttcggg agagggtggc attatctggt gagagaattt
    10621 ggcatgaaac ttaaaataat aacagggtaa gcttcacaaa tttgcacttt taagaaattg
    10681 ggtagtatga gagcctatta agatattcat taaacttatt gtccgggctt tcacaaaaaa
    10741 ttattaattt ttctactaat taaaaagttg tgtgcttttt gtacaatgat tatataagcg
    10801 tagcgccctg ttgtgatatt gaaatgttaa cataaccaaa ataaattgat ttatagaata
    10861 tataaaattc attaatgggg tatatgttta atatataaat aagatggatg gctttgatgg
    10921 agggatatta acggtttact cattgtctca ggcttcactg aaacgggtaa ggaagcgtaa
    10981 aaacgaaaaa accgcaagcc aatagtagct ttgcggtttg tcagcattct aataaattgg
    11041 ttgcgggggc cggatttgaa ccgacgacct tcgggttatg agcccgacga gctaccaggc
    11101 tgctccaccc cgcgtccgtg gatgcgcact atactctgct cgcgttttca tgcaaccttt
    11161 tttttacccc gatcacggat tcggcatgat tttgaacaaa aacaaatcac taaagcctat
    11221 tttttgtgca aaatttgcgt cagggagagg ctgctcattt cattaaacgc ggtggtttgt
    11281 tatcttcgtt gcgccttatt ttttaacctg aagaagagaa caatgaaagg acgttgggta
    11341 aagtaccttc ttatgggcac ggttgtggca atgcttgccg cctgctcttc caaaccaacc
    11401 gatcgcggac agcaatataa agacgggaaa tttacccagc ctttctctct ggtgaaccag
    11461 ccagatgccg ttggcgcgcc gattaatgcc ggtgattttg ccgagcaaat taaccatatc
    11521 cgtaattcgt caccgcgtct gtatggcaac cagagtaatg tttataacgc ggtgcaagag
    11581 tggctgcgcg caggcggtga tacccgcaat atgcgccagt tcggcattga tgcctggcag
    11641 atggaaggtg tcgacaacta tggtaacgtg cagtttacag gctattacac gccggtaatt
    11701 caggcgcgcc atacccgtca gggcgagttc cagtatccga tttatcgtat gccgccaaaa
    11761 cgtggtcgtc tgccgtctcg tgcggagatc tacgcaggag cgctgagtga taaatatatt
    11821 ctcgcttaca gtaactccct gatggataac ttcattatgg atgtgcaggg tagcgggtat
    11881 atcgactttg gtgatggcag tccgcttaac tttttcagct atgcagggaa aaacggtcat
    11941 gcctatcgca gcattggtaa ggtgttgatc gaccgtggcg aagtgaaaaa agaagatatg
    12001 tcgatgcagg cgattcgtca ctggggcgaa acacacagtg aagcggaggt tcgcgagctg
    12061 ctggaacaga acccgtcttt cgtcttcttt aaaccgcaat cttttgcacc ggtgaaaggg
    12121 gcaagtgcgg tgccgctggt tggccgtgct tctgttgcct ccgatcgttc aattattccg
    12181 ccaggtacta ccttgctggc agaagtgccg ctgctggata ataacggcaa atttaacggt
    12241 cagtacgaat tgcgtctgat ggtggcgctg gatgtcggtg gcgcaatcaa aggccaacac
    12301 ttcgatatct atcaagggat cgggccggaa gccggacacc gcgcaggttg gtacaaccac
    12361 tatggacgtg tctgggtgct gaaaaccgcc ccgggcgcag gtaacgtctt tagcggctga
    12421 tgtgatattc tgtatgccgt ttttacggat atgacagggt gaggggtaac ctcacccttt
    12481 ttaattctga ggtgctatgt ctgtggtaat tagtgatgca tggcgtcagc gttttggtgg
    12541 cacagcgcgt ctgtatggtg aaaaagcatt gcaactgttt gctgacgcgc atatttgtgt
    12601 ggttggtatc ggtggtgtcg gttcctgggc ggcggaagcg ctagcgcgca cggggattgg
    12661 cgcaatcacg cttatcgata tggatgatgt gtgcgtcacc aataccaatc ggcaaattca
    12721 cgctctgcgc gataacgtcg ggctggcaaa agcggaagta atggcggagc gtattcgcca
    12781 gattaacccg gagtgccgtg taacagtagt ggatgacttc gtgacgccgg ataacgtagc
    12841 gcaatatatg aatgccggtt attcgtacgt gattgatgct attgatagtg tacggcccaa
    12901 agcggcgctg attgcttatt gtcggcgcaa taaaatcccg ctggtcacga ccggtggcgc
    12961 tggtgggcag attgacccga cgcagattca ggttaccgat ctggcgaaaa cgattcagga
    13021 cccgttggcg gcgaagttgc gcgagcgcct gaaaagcgat tttggcgtag tgaaaaacag
    13081 taaaggtaag ctcggcgtgg attgcgtgtt ctctaccgaa gcgctggtgt acccgcagtc
    13141 agacggcacc gtctgtgcga tgaaagccac ggcggaaggg ccaaagcgga tggattgtgc
    13201 ctcaggattt ggcgcggcga cgatggtgac cgccactttt ggctttgttg cggtttctca
    13261 tgcgctgaag aagatgatgg cgaaagcggc gcgtcagggg taaacaatca ggtcggatgc
    13321 gacgcgaacg tcttatccga cctggttctg tgcttaaacc tgcttcgccg cagcgataat
    13381 cgcctcgctt aacgcattca acccctggct gcgtgaggcg ctaagttgcg cacgtaatcc
    13441 cagctcatca aacaatgcca gcggcgactg tgcctgcaat tcggcggcgg ttttcccctc
    13501 aacggcagtc aacaacaccg ccaacaggcc gcgcacaatg cgcccttcgc tgtcgccaaa
    13561 gaaatgcatc ttgccgtttt cagccactgt atatcccagc cagacgcggt tttcgcatcc
    13621 ggcaatctct ttagcctgcg cttttaactc ctctggcaaa gccggaagct gtttccccag
    13681 catgatcaac tggcgatatt tatcttccca ttgcgtcaac ggtgcgaagg tattgcgtaa
    13741 cgtttctgcg gttacggttg tgccgaacgg atgtccggcg aattgcgggt ttgtcattaa
    13801 tccaccaata attccagcgc gcggtcaacg gcattcacca gcgcatccac atcactcttt
    13861 gtattatatg gcgcaaatga ggcgcgcagt gtgccggtta cgcctaattc tgccagtagc
    13921 ggctgagcgc aatgctgccc ggcccgcagg gcaataccgt actccgccag cagcgtcacc
    13981 atatcgctgt gatgaacgcc agcaaaatca aaggccagca ggctggaatc ctggcagcga
    14041 aatgagcgga agccaggacg ttttgccagc gcatcttccg ccagcgttgc taagctacgg
    14101 ctccagcttt cggcctggtt gatatcgtaa tctgccagcc attccagcgc cgcgcttaat
    14161 cctatgacac cagcgacatt tggcgttcca gcttccagtt tccacggcgc agattgaggc
    14221 gtgaagccgt caaaactcac ttcgtgaacc attttgccgc cgcccagcca gggcgacatc
    14281 gcttccagca gttctgattt gccatacagc acgccgatac ctgtcgggcc atacagtttg
    14341 tgacctgaaa aagcatagaa atcaatatcc agttgctgaa catccgcggg gaaatgcacc
    14401 gccccctgag caccatcaac catcaccacc atcccggctg aatgagcaaa ggtaatcgct
    14461 cgcgccagat ccgggcaacc gccagtgacg ttcgacattt gtcccagagc aagaattcga
    14521 ctacgaggtg tgatcagttc tggcaacaaa tcgacatccg gcagtcgctg cgcattaagc
    14581 ggcaatttca ccactttggc tccagtttgt tgggcgacca tcagccaggg gacgaggttg
    14641 gcgtggtgtt ctgccacgct gacaataatc tcatcgcccg gttgcagatg cggacgcgca
    14701 tagcattgcg ccaccatgtt gatggattca gtggtgccgc gcgtccagac gatagttttg
    14761 tcatctggtg cattcagtaa ttgcgccact ttctcccgtg cagcttcata acgcgcggtc
    14821 aggcgttggg cttcggcaaa ctggctgcga tggacgtttc cggcgctcag gctgtaaaac
    14881 tgtcgggtgg cttcaaccac ggcttcaggt ttaagcgtgg tcgcggcgct gtcgagatag
    14941 acgcccgcat cctgtagcgc gggaaactgg gcgcgaaact gcgcgggatt aaaaacgttc
    15001 atggtactcc tcggcttgat gcaccgatcg tgacgcaaaa ttcactttga tacaaggaga
    15061 gtgctaacca tcagaattat ctggatatcc tggcgaagcc gcgtcagtaa catatgctga
    15121 atagtgttag gcgttaattt tagcctgtta atcaatacta ttaagattta ttaatcgctt
    15181 taaggagaag atgatgaaaa agactgccgc aattatttct gcctgtatgc tgacttttgc
    15241 cctgagcgcc tgttccggtt cgaactatgt gatgcacacc aatgacggac gtaccatcgt
    15301 ctctgacggc aaaccacaga ctgataacga taccggtatg atttcgtata aagacgctaa
    15361 tggcaacaaa cagcagatca accgtaccga cgtgaaagag atggtcgaac tggatcagta
    15421 attcgcgatc gcaaggtaaa aaaaagcacc gcaattaggc ggtgctacat taatcactat
    15481 ggacagacag ggtaaatgta caggaagtga aaaaaggtag ctttgctacc atggtctgaa
    15541 tcgcagacca attgcaaaca caacaacaca acatcacaac cgtaagccaa aagttcacca
    15601 gaacacgcat tccgataaaa cttttcgttc cggctcagga agtgccgcca ctataggtat
    15661 ttgctggtag aagctcaacg gacaatttat aatggctcag attaaaaaaa ctaataggtt
    15721 acatagtgta atctaattgt taaattcatt taacatcaaa gtttaaaagc catgtctaaa
    15781 cgattaccac cgctaaatgc cttacgagtt tttgatgccg cagcacgcca tttaagtttc
    15841 actcgcgcag cagaagagct ttttgtgacc caggccgcag taagtcatca aatcaagtct
    15901 cttgaggatt ttctggggct aaaactgttc cgccgccgta atcgttcact cctgctgacc
    15961 gaggaagggc aaagctattt cctcgatatc aaagagatat tttcgcaatt aaccgaagcg
    16021 acgcgtaaac tccaggcccg tagcgctaag ggggcgttga cggtcagttt actccccagt
    16081 ttcgccattc attggttggt tccgcgactt tccagcttta attcagctta tccgggaatt
    16141 gacgttcgaa tccaggcggt tgatcgtcag gaagataagc tggcggatga tgttgatgtg
    16201 gcgatatttt atggtcgggg caactggccg gggctacggg tggaaaaact ttacgccgaa
    16261 tatttattgc cggtgtgttc gccgctactg ctgactggcg aaaaaccctt gaagaccccg
    16321 gaagatctgg ctaaacatac gttattacat gatgcgtcgc gtcgtgactg gcagacatat
    16381 acccgacagt tggggttaaa tcatatcaac gttcagcaag ggccaatttt tagccatagc
    16441 gccatggtgc tgcaagcggc tatccacggg cagggagtgg cgctggcaaa taacgtgatg
    16501 gcgcaatctg aaattgaggc cggacgtctt gtttgcccgt ttaatgatgt tctggtcagt
    16561 aaaaacgctt tttatctggt ttgtcatgac agccaggcag aactgggtaa aatagccgcc
    16621 tttcgccaat ggatcctggc gaaagccgct gctgaacaag aaaaattccg ctttcgttat
    16681 gaacaataat ttacgtaggg tacgaccatg accagccgtt ttatgctgat tttcgccgcc
    16741 attagcggct tcatttttgt agctctgggc gcttttggcg cgcatgtgtt aagtaaaacc
    16801 atgggggccg ttgagatggg ctggatccag accggcctcg aataccaggc gtttcatacg
    16861 ctggcgatct taggtctggc ggtggcaatg cagcgtcgca tcagtatctg gttttactgg
    16921 agtagcgttt tcctcgcgtt aggcacggtg ctgttcagcg gcagccttta ttgcctggcg
    16981 ctgtcccatc tgcgtttgtg ggcgtttgtc acaccggttg gcggcgtgag cttccttgcg
    17041 ggctgggcgt taatgttagt tggtgctatc cgtttaaagc gcaagggcgt aagtcatgaa
    17101 taaggttgta ttgctgtgcc gtccgggctt tgaaaaagag tgcgccgcag aaattaccga
    17161 taaagccggc cagcgggaaa ttttcggttt tgcccgcgtg aaagagaatg cgggttatgt
    17221 catttatgaa tgttatcaac ctgatgatgg cgataagtta atccgtgagc tgccgttcag
    17281 ttcattaatt tttgcccgcc agtggtttgt ggtgggggaa ctcctgcagc atttgccgcc
    17341 agaagatcgt attaccccca ttgtcggcat gttacagggt gtagtagaga agggcggtga
    17401 actgcgtgtt gaagttgccg ataccaacga aagcaaagag ttgctgaaat tctgccgtaa
    17461 attcaccgtg ccgctacgcg ctgccttgcg cgatgcgggt gtgctggcga actatgaaac
    17521 gccgaagcgt ccggttgtgc atgtattctt tattgcacca ggctgctgct ataccggtta
    17581 ctcatacagc aacaataatt cgccgttcta tatgggcatt ccgcgcctga aatttccggc
    17641 agatgcgccg agtcgttcca cgctcaaact ggaagaggca tttcatgtgt ttattcctgc
    17701 ggatgagtgg gatgaacgcc tggcgaacgg gatgtgggcg gtggatttag gcgcttgccc
    17761 aggcggctgg acctaccaac tggtgaagcg caacatgtgg gtttattccg tcgacaacgg
    17821 cccgatggcg caaagtctga tggataccgg acaggtgacg tggctgcggg aagacggttt
    17881 caaattccgt ccgacacgca gcaatatctc ctggatggta tgcgatatgg ttgaaaaacc
    17941 ggcgaaagtt gcggcattga tggcgcagtg gctggttaat ggctggtgcc gtgaaaccat
    18001 tttcaacctc aaactgccga tgaaaaaacg ctacgaagaa gtgtcacaca atctggcgta
    18061 tattcaggca cagcttgatg aacatggcat aaatgctcag attcaggcgc ggcagttgta
    18121 tcacgatcgc gaagaagtga cggtgcacgt ccgccgtatc tgggctgcgg tgggtggtcg
    18181 tcgcgacgag cgataacagg caaggccggg gcgtcgtttc cggcctgcta tcctttccgt
    18241 caggctgtta ccaaagaagc tgcaacctgg cgctcatcag aaataatgtg cgtgacctca
    18301 tccagatgcc cgatgcgggc ttcccctgaa cgattaaatt tacttttatc aatcaataac
    18361 aacgattgcg cggcacgttt taatagcatc gatttgtaat cagcgttgat ggcattggag
    18421 tcccacagtg cgccgctgct atcgatccct tcacaagaaa aaataaacag atcgatttcc
    18481 agcgatttaa gctgggaaat cagcgaggga ttgacgtaac aaccatattt gcgctcaagt
    18541 gtgccgccgg aactgatcag ttgaatgcgt tcgcgtttac cgagttcatg gcaaatcgga
    18601 tggctattgg tgaagacctg gatgttgatg tcaggcaact ggcgtgccag ataccagcag
    18661 gttgaacttg cgtctaaggc tattaccatc ccttcttcaa tccacgcgag cgcctcgcgc
    18721 gcgatatctg ctttatgcgc ataatggctt ttcagccgga tgtgaaaggg atcgccactg
    18781 tcttgatttt gacggtggat atatttagcg cgcccatgat tgcgcagaat tttaccctgc
    18841 gtctgtaatt cattgagatc gcgacgaatg gtttctttac tgacctttag ctgttcagag
    18901 agagcttccg tggtcaggct ggtatggttc agcagcaggt cgactatcgc ttgctggcgt
    18961 gccgctttca tatttttcac cccatcattc gataaccgcc cgcgtcatgc gggcgtaccg
    19021 gtatgagatt acggtgttac cccttttttt aaaagaatat tcccgtactt cgctcgttcg
    19081 cctgtgataa cgatcgcaaa ggctttttgt gcccgttcat aaaacgcaaa acgattgatg
    19141 cggatgatgt caggacaggg ggcttgtagt gaaagcgcat tacgataacg tcgttctact
    19201 tcaggatcta gagtgtcacc ttctaccgcc gccatcatca ccagcggtgg tgcataactg
    19261 tccagctcaa ataacgggat aatcgcctgg agcaagtcgc tcaccaacag gccatcagcg
    19321 cggatcacct gcggtcccat cgaatgggcg ggaaagtgag catcggaaaa aataatttca
    19381 tctccatgcc ccatctctgc cagcactttc aatagttcgg gagaaattaa cggcgaaatt
    19441 gttttcagca tttcacacct cctctataaa ttcaggttca gtttgcgggt agaaataacg
    19501 gtactgataa tgaatctgcg cgcgggcttc ttccggactg ttaaattccc ctacgccata
    19561 ccaaccaaac agcgcagctc ctgcgacggt cgtttcggca tcgtcgagaa cttttaccgg
    19621 aatatcaagc atattggctt taatctgatt ccacaatgtg ttgcgacttc ctccgccgac
    19681 taacaataat tcagaggctt taaagtgccc gattttttcc agcatctgta gattgcgctg
    19741 taattgcgtg gttaaccctt ccagcgccgc gcgatagaaa tgcccccgcg tggtattaag
    19801 cgttacgcct tgccagccag cgttctggca cgacaataaa tcacactgca ttttcactcc
    19861 atctgcgcca ggcgcgatca ggcgagcttc ttcaatcaat atttgccagg gagtttcagc
    19921 tgtccagaac agttttctca cccattccag cacgccggat gccagccatt gcatacctgg
    19981 gttatacaac cctgcctggc tatccagttc gcaagtagaa ccggcgtact gacttaacag
    20041 cgaagtatca acctgggcgc tgcggaccat taaaatttcc catgtaccgg aagagagcac
    20101 gggttcattt tgttcggcac cagcgccaaa aagggcgaac tgggtatcgt gacctgcgga
    20161 aatcaccggt atgccaacgg gtaagccgag cattgctgcg gcgctgttct gtagcgtacc
    20221 aatctgttca cccgcttcca ccagacgagg gaagagtcgg cgtggaatac cggtggcttg
    20281 taaaatttgc ggactgaaat cgcgttgctg gatatccagc atctggctgg ttccggccat
    20341 cgtaatatca gtagtgaatt cgccggttaa acggtggtta atcagtgacg aaataaagag
    20401 ccaggcgtgc gcgcgttcca gcagttgtgg atgattttct ttcaaccaca ccaacttata
    20461 taacgtattg aaactaaagg ctccgactcc agaaatagcc tgcaactgct gtgcggagat
    20521 caaccgttca atattgtcca ttaccgcggc tgttcgcgga catttccagc taatgatcgg
    20581 atagagcaga ttgccttgct tatctaccag agcgccatcc acaccaaagg tggtgacggc
    20641 gataccgcgg atatggcaat cagtcagttc actattgatt tgccgacagc aatcagcaaa
    20701 gcgttgcaaa atggcgtcta aagaccactg gtgccaggtg ttgttttcca tcgcgatatc
    20761 gctggcatta ggcgttgagg cgcgggcaac aatttttccc tgccgattaa ccgcgatggc
    20821 cctgacattg gtcgcgccac agtcgagtac caggataact tcttgtttca taatcgctcc
    20881 tgcaatatag ccggataaca ttgcttatcc ggctaaccac tcttgcttgt tcaggcccgg
    20941 tagccggagc gaccgggcat cacatcaggg agtcatgtat taacgcttgt acaacgggcc
    21001 gtagttctgg caagcgcggt aatcctggcc ttcaatatcc atgccgtgcg cggcccaggc
    21061 agaaggacga tacactttgg tctcttcaac gttgtgcata cataccggga tacgcagcat
    21121 ggaggcaaga gtgataaagt ctgcgccaac gtggccgatg gtcagaactc cgtggttagc
    21181 gccccagttc gccattaccg agtacacatc cgtaaacggc cctttaccgg tgaggcgcgg
    21241 tgcaaaccag gtggttggcc aggttgagtt ggtgcgtttg ttgaggatgt catgcacatc
    21301 cttcggcaat tccacgctcc agccttccgc gatttgcagt accggtccca ggcctttgat
    21361 gatgttgaca cgagtcatgg tgaacgggac gccgccttcg gtaaggaagc gggaagagta
    21421 accgccgcca cggaagtatt cgtggatcgc cgggcaccat tcggtagcgg cgaggcaagc
    21481 gtcagcctct tgctgagaga tttcccagtg cggcttcatc gtcggtttac cttcgctgtc
    21541 gcgttgttta caggagccgt ccagcgcagc agaaccggag ttgatcaaat ggatgatgcc
    21601 gtgttctgcc agcccgtcca gtttatgccc cgttacacgc tcaattgctt ctggtgacca
    21661 gtaggtacgc acatcggcaa atatctgagc ggtgccggtg agctggtggc ccattaacat
    21721 tgccacgccg ttaagactgt cgttttcggt cgccacgaca aacggtttgc gcacgccatt
    21781 ccagtcaaat gaactgttga ggatcgcttc agcggtatcg ccattgggat attgatcggt
    21841 ccagtgacgt tgcccctgga agcctgcagc gatggcgttg tagccaagtg attcttccac
    21901 gcggccaata tcggccagtt tgctgttgcc ttgcatcatg tcgcggatac acatcgccat
    21961 cagtaaactt tcgcgcagaa ctgcgcggct ttgctcggca ttgcgttgat actgtttgtt
    22021 attttcatct tcgccatagc ggaagttttt atcagcccag gccagcgcca tttccagttc
    22081 ggcttcgtcg taaatcttct gatcgatacg gcgacgcagt tcggtcatat ccaccgcctg
    22141 gactttcatt cccagccagg attcaaagaa gttgtgatca acgatggaac cggcgatacc
    22201 catcgaaacg ccgcccagcg acagatagct tttacctttc atgctggcga ctgctaaacc
    22261 ggcgcgggca aagcgcagca gtttttcttc aacatcggca ggaatcgatg tgtcatcggc
    22321 atcctgaacg tcatgaccgt aaatggagaa tgccgggatg cctttctggc tgtgagctgc
    22381 cagagccgct gccaggtaaa cagcgccggg acgttcagtg ccgttaaagc cccaaatggc
    22441 tttcgggcgg gttggatcca tatcgatggt ttcactgcca tagcaccagc aaggggttac
    22501 cgtaatggtg aggcctacat tctgactgct gaatttttct tcgcaagcag cggcttcagc
    22561 catacccgcg atacaggtat cggaaatgac acactcgacg gcagctccac aggcatggcg
    22621 cagtttctcg gtcagcagtg cagccgtggc tttcgccata ttcattgttt gttcttcaag
    22681 cgactcacga acacccatgc gacgaccgtc aataaccggg cggataccaa ttttcggtaa
    22741 gctgattttt ttcattacag attcctcact ttattcggaa aatatgttca gttagttgct
    22801 gtttgagaac ggaaacgggc aaagataaag atgaccgcga agcagagtgc ggggatcagt
    22861 tcagcagtgg ggatgttgcc cgccgcgtca ctgacaaaac ccatgaccgg agtgacaata
    22921 ccgccgccaa tgatggtcat aacgatgaag gacgaaccgt atttggtgtc ctggccgaga
    22981 ttcttaatgc ccagcgagaa gattgttggg tactgaatcg acataaaggc gctgcataaa
    23041 gtcagggcta ttaagcccac atgaccgcca gcgaaggctg agatcaggca cagtgccata
    23101 gcgattaatg cgtaggctgc caggactttg tgtggtgcga agcgactgat gagccaggta
    23161 ccggtgaaac gaccaataaa gaagcacacc atggttccgg ttaaatagtt agcggcaaag
    23221 cctgcggtca tacctggaat ttcttctaca gcgtagcgaa tcaaatagct ccagcaggcc
    23281 gtttgcgcgc cgacatagca gaattgcgct aataccgccc agcgccagtg acgaatacgc
    23341 gccagacgag aaagcgatgc ggagaacgat ccttgtttgg cgtcactgtg attatcactc
    23401 tgcaatgccg ggaatttcgt cagcatgatc agcagggcga ccagtaacac gatagccacg
    23461 atgatcatat aaggtgtctg taccgataat accaggctgt gtttatacgc actcaattgc
    23521 tctggagaca ttttatcgag aacgtcttgc gattgatgtg gcacgttaga caaaataaga
    23581 ctttgtccaa agacaaccgc gataattgcg ccaaacgagt taaatgtttg cgcaagattt
    23641 aagcggaagt gaccactgct ttccggccct aataccgtaa caaaagggtt tgcggcagtt
    23701 tccagacaac ctaatccagc tgcaataata aataggccaa ctaaaaataa ggtgtagttc
    23761 attatttctg cagcgggcca gaataatgca gcgcccaagg catataaaaa taatccggta
    23821 ataatccctg ctttataact gagttttttc atcaatatcc cagcagggat tgggataatg
    23881 aaataaccaa agtaaaaggc cgattggatc aggccagcct ggaaatttgt cagcgtaaaa
    23941 gcctgctgga attgaggtaa taaaatgtcg ttaaggttat tggctaccgc ccaaagaaaa
    24001 aacagtgagc acagcagcgc gaatggaata atgtaacttc tgctttgccc tgcatcttta
    24061 tctaccgcac ggtaactctg cgtttgtatt gatgtgtttc ccatagcatc ctcttaggtt
    24121 cagaagctta agcacgtaat cggttatgtt ttcaaaagtg caaaaccagc gagttcgggc
    24181 ttcatatcag tgaatatttg cctgtcagaa atgaactcat ggattcattt tcaaataaaa
    24241 aacgctctgt aatatgacgg cggtcacact tattagttgg gaggtgactt tatgtgacta
    24301 ccatcacttt aataatcgaa atttcatgcc cgtttcagaa aataaatcat aaaaaacaca
    24361 aacccgatga ccgtattaat tgtttgtgcc gaaaacgggc atttctggcg agggggaatg
    24421 gaaaaatgac cgttttgata aatattttta agagcggtca tttttaatag ggttataaaa
    24481 agagaacttg cagttattta ctgtgattag ttgaaccagg tcacaaaata taagcttgtg
    24541 aatttctttc tggttaatca ggaagaagat ggtgggtaat taaacggcta attcaatagt
    24601 gtgaaaggaa caacattatt gccctgtttt gaatcagaga gaggtagcta atggaacgaa
    24661 ataaacttgc tcgtcagatt attgacactt gcctggaaat gacccgcctg ggactgaacc
    24721 aggggacagc gggtaacgtc agtgtacgtt atcaggatgg gatgctgatt acgcctacag
    24781 gcattccata tgaaaaactg acggagtcgc atattgtctt tattgatggc aacggcaaac
    24841 atgaggaagg aaaactcccc tcaagcgagt ggcgtttcca tatggcggcc tatcaaagca
    24901 gaccggatgc caacgcggtt gttcacaatc atgccgttca ttgtacggca gtttccattc
    24961 ttaaccgacc gatccccgcg attcactaca tgattgcggc ggctggcggt aattctattc
    25021 cttgcgcgcc ttatgcgacc tttggaacac gcgaactttc tgaacatgtt gcgctggctc
    25081 tcaaaaatcg taaggcaact ttgttacaac atcatgggct tatcgcttgt gaggcgaatc
    25141 tggagaaagc gttatggctg gcgcatgaag ttgaagtgct ggcacaactt tacctgacga
    25201 ccctggcgat tacggacccg gtgccagtgc tgagcgatga agagattgcc gtagtgctgg
    25261 agaaattcaa aacctacggg ttacggattg aagagtaatt tcgtaaagca acaaggagaa
    25321 ggatgatggc taacagaatg attctgaacg aaacggcatg gtttggtcgg ggggctgttg
    25381 ggtctttaac cgatgaggtg aaacgccgtg gttatcagaa ggcgctgatc gtcaccgata
    25441 aaacgctggt gcaatgcggc gtggtggcaa aagtgaccga taagatggat gctgcagggc
    25501 tggcatgggc gatttacgac ggcgtagtgc cgaacccaac aattactgtc gtcaaagaag
    25561 ggctcggtgt attccagaat agcggcgcgg attacctgat cgctattggt ggcggttctc
    25621 cacaggatac ttgtaaagcg attggcatta tcagcaacaa cccggagttt gccgatgtgc
    25681 gtagcctgga agggctttcc ccgaccaata aacccagtgt accgattctg gcaatcccca
    25741 ccacagcagg tactgcggca gaagtgacca ttaactacgt gatcactgac gaagaaaaac
    25801 ggcgcaagtt tgtttgcgtt gatccgcatg atatcccgca ggtggcgttt attgacgctg
    25861 acatgatgga tggtatgcct ccagcgctga aagctgcgac gggtgtcgat gcgctcactc
    25921 atgctattga ggggtatatt acccgtggcg cgtgggcgct aaccgatgcg ctgcacatta
    25981 aagcgattga aatcattgct ggggcgctgc gaggatcggt tgctggtgat aaggatgccg
    26041 gagaagaaat ggcgctcggg cagtatgttg cgggtatggg attctcgaat gttgggttag
    26101 ggttggtgca tggtatggcg catccactgg gcgcgtttta caacacgcca cacggtgttg
    26161 cgaacgccat cctgttaccg catgttatgc gttataacgc tgactttacc ggtgagaagt
    26221 accgcgatat cgcacgcgtt atgggcgtga aagtggaagg tatgagcctg gaagaggcgc
    26281 gtaatgccgc tgttgaagcg gtgtttgctc tcaaccgtga tgtcggtatt ccgccacatt
    26341 tacgtgatgt tggtgtacgt aaggaagata ttccgacact ggcgcaggcg gcactggatg
    26401 atgtttgtac cggtggcaac ccgcgtgaag caacgcttga ggatattgta gagctttacc
    26461 ataccgcctg gtaaatgcgc tgatgtgata atgccggatg cgacgtttgc gccgtatccg
    26521 gcacgccgtt accttaccaa ccgcaattgc tgtaaattgc cgtcgatatg caaatcggtt
    26581 tgtaagcggg caatatcgcg gcacagaaac gccatctctt tatgggtttc taattttttg
    26641 cgccactttt cggcaaccgc attcagattc tcatatatcc cttccagact cttaaactcg
    26701 accagcagct gcgtggcgct ttttggtccg atccccacaa cacccggtac ctttgaactg
    26761 ctgatccccg caagtcccca gtaatcgggc aactgctgcg gttgaacgcc aaattcttta
    26821 tcgataaatg gcgcatccag ccaacgtttc tggaagtaat cacgaatacg taatgtcggt
    26881 gaaagtaact gacagtagcc tttatctgtc gaaacaatcg ttgcctgatg cccggcctgt
    26941 gtcactttga ccgccagcgt ggcggctaag tcatcggctt cgttgccgct ggcggaccag
    27001 cacgggacgc cgcgttgctc aaaggcggcg cgtaatgcag gcatctcgtc gtgcaactct
    27061 tccggcattg gcggtcgacc cgctttgtaa tctggtaaac gctgatgacg ccagccgcta
    27121 ctgcggtttt catcatcaaa aacggcgacc gcgtgggttg gctggctgtg cataatgagc
    27181 tgatcgagcg cgtgctggca ggtttcgaca cagggcgaac cctgaacggc atgaatgcga
    27241 cgaataagat tcagtgcatc gacaataagc aaatgaacag ccacgataac ccccttaaag
    27301 atcaggcgat aagggtaaca ttcgatgccg gaacaggcta tggctggttg agaaaacggg
    27361 aagaggcctc gcaaaacgag gcctccggga gagattaatc gcaggcaacg atcttcattg
    27421 ccaggccacc gcgagaggtt tcgcggtact tggcgttcat atctttacct gtttcgtaca
    27481 tggtctcgat aactttatcg aggcagacgc gcggctcgct ggtacggcgc agcgccatac
    27541 gcgcggcgtt caccgctttt actgccgcaa tggcgttacg ctcgatgcac ggtacctgca
    27601 cctgtccagc aaccgggtca cacgtcagac cgaggttatg ctccatggcg atttccgccg
    27661 cgatgcacac ctgcgccggg cttgcgccta acagttctgc cagaccagct gccgccatag
    27721 agcacgccac gccaacttca ccctggcaac ccacttcggc accagaaatc gacgcgttca
    27781 tcttataaag agaaccaatg gcgctggcta ccagcagata acgagccagt gagttagcgt
    27841 tcacttcgcg gataaactta tcgtagtacg ccagcacagc cgggataatc ccgcacgcac
    27901 cgttagtcgg cgcagtcacc acgcgaccgc cagcagcatt ctcttcgttc actgccagtg
    27961 caaacatgtt gatccagtca acaaccgcca tcgggtcagt ggtggtttta tcctggctga
    28021 ccagcatccg gcgtagtgcc gcagcacggc gtggaacgcg cagtttgcca ggtaacacgc
    28081 cttcggtaga aataccacgc tcaataccgc cacgcatgac ttcccagacg ttcgccaggt
    28141 gctgttccag ctcttctttg ctgtgcagcg ccagctcgtt tttcatcatc aggccagaga
    28201 gtgacagccc ggtttcctga caatgttttt gcagatcggc tgctgaactg tacggataag
    28261 gaacttcaac cggtgcgcta tcctgctggc caaaatgttc ttcatcaacg ataaagccac
    28321 cgccgataga gtagtaagtc tggctgtaaa cgactttatc gcccgccagt gcggtaatgc
    28381 gcataccgtt ttcatgcaga gaaaggttgt cggcatggaa gttcatgcac tgatcaaccg
    28441 ggaactccac ttcatgctga ccgtttgcca gcatcaggcg accatgagta ttcacatcct
    28501 gaataaaacc agggatggaa tcgatatcca cggtatccgg caggttaccc gccaggccca
    28561 taataatggc gatatcagtg tggtggcctt taccggtcag agagagcgag ccgtacacgt
    28621 caaccaccac gcgggtcacg tctttaagca ggttacgggc aatcagatcg tcggtaaatt
    28681 gtttacccgc tttcattggt ccaacggtat gagaactgga agggccaatg ccgattttga
    28741 aaatatcgaa tacgctaatc ataggaaata catcgcgtta aaacggagga agcgccgccc
    28801 gaaagcggcg cgaaaggact tagctgaaca gagagtagaa gattgcggag attgcaatca
    28861 gacccatcac gacaacgaat acgttgctga tgtgaccgct gtacttacgc attgccggta
    28921 ctttctgaat tgcgtacatc ggcatcagga acaggatcat cgcgatgatt gggccgccca
    28981 gggtttcaat catacccagg atgctcgggt tcagggtggc aacaatccag gtcgttacca
    29041 gcatgaacag cgcagtgata cggttcagct tgttgatttc gatagactta cctttaccac
    29101 gcagagattt aatcaccata ccgttgaagc cttcacgtgc gcccaggtag tggccgagga
    29161 aggatttggt gatagcgata atcgcgataa tcggagccat ccacgcgatg atcggcgcgt
    29221 taaagtggtt agccaggtaa gacagaatcg agatgttctg ctctttagcc gcagccaggt
    29281 ctgctggagt caggctcagt acacagctga agacgaagaa cattacggtc agcaccatca
    29341 tgatgtgtgc gaatgccagg atcttcgagc atttctgttc tgccatatcg ccgtactctt
    29401 cacgcttcgc aacggcgaaa gaagagatga tcggagagtg gttgaacgag aacaccatta
    29461 ccggaattgc cagccacagg gtcatccaaa gaccgtttcc ggttgcagat gcagtgtcca
    29521 gagacagcgt ttccagtgct gcgccgttcc actgcgggat caggtacaga gccagcagca
    29581 tcagtacgcc aacaaacggg aataccagaa tactcatcgc tttaacgatc atctgctcac
    29641 cgaagcgaac gatggtcatc atacccacga tcaggatcag cgacagaatc gcacgcggcg
    29701 gtggtgtcat acccagctgg tgagacatga agctttcaac tgtattggtg attgccacgc
    29761 tataaaccag cagaatcggg tagatagcga agaagtagag cagggtaatc agtttacctg
    29821 cgccaatacc aaagtgttct tctacaacct cggtgatgtc ttcgcccggg tttttaccag
    29881 acagtacgaa gcgagtcaga ccgcggtgag caaaaaatgt catcgggaac gcaaggatag
    29941 ccatgatgat cagcgggatc ataccgccaa caccggcgtt gattggcagg aacagcacgc
    30001 ccgcgccgat tgccgtgccg taaaggccca gcatccacat ggtgtctgtc ttgcgccagg
    30061 cactacgaga gtctttcgac gcaatcgtgc tggtttgagt cgtttccatc tatttctcct
    30121 ggaggaatgc aaataattca gcttttagcc agttcagccc gggaaggtgc ctgaccgtaa
    30181 aaatgaaatc tgtctatagg cgatggctta atcatttacc gccgtaataa tttgagggcg
    30241 gaaagataca cacaagttat gtatctatca gtgatcttga tcccaaatac aataatctac
    30301 ttggaagata gattatccca acgctttact ctgtaaatca aaagtaaaag agaatttaag
    30361 ggcaatattt gcccattaaa cgacatctga cttcttccgg ggcgcgcaat atatgtaggt
    30421 tgaactgaaa aaatcaatac ttttagttaa tcatggcgat aatttctttt tcatagcgat
    30481 aaaatgtggt ttatgaaaat agggaaacgt ttgcgttaca acataaattg tatgacatat
    30541 taactgtaat gaaatttatc gcatgagggt ggtaagaaac attcatataa caaatgaaat
    30601 aaattgatgg gtatgtgacc aaaaacgaga aaacccggca acaaacgtca ccgggggagg
    30661 gagattacgt gcagatttcg tagcaaggga tgtaggctga gcctggcaac ttcatacgat
    30721 gctgggcaac aaaaccctgt agcaggtcat ccatacgacg cataatctct ttatcgccgt
    30781 tgattttata aggaccaaac tcttcaatgg cgcgaatacc gacttctttt acgttacccg
    30841 ccacaatacc ggagaacgca cggcgcaggt cggcagccag cacttcaacg ggttgatccg
    30901 ggtaaagctt cagattagcc atattctcgt gagacggctc aaacggcatt tgcaaatctg
    30961 gcgcaatgcg cattgaccag ttaaagctgt aggcatcgcc tgtgtcgcgg cgattttctt
    31021 tcaccagcgg catcgatttt ttcatctgac gagcgacttc agcggcgtca tcaatgatga
    31081 tgcggtaatg gcggcgcgcg ttttcaccca atgtatgcac gacaaactcg tccagtacgc
    31141 ggaagtagtc ggcgctctct ttcgggccgg tgaggatcaa tggtaaaacc tgatctttgt
    31201 tggccgggtt cattaaaatt cccagcaaat acagcaactc ttctgccgta cccacaccgc
    31261 cagggaagat aatgataccg tgagcgatac ggacaaacgc ttccagacgt ttttcgatat
    31321 ccggcatgat gatcaattcg ttgaccagcg ggttaggcgg ttcagcggcg ataatcgacg
    31381 gctctgtcat accaataaaa cgactgtctt tgtaacgctg ctgtgcgtgt ccgaccgcag
    31441 cgcctttcat cggcgcttcc atcgctcccg gaccacagcc ggtgcagata ttcagctcac
    31501 gcaggcccag ctgattcccg acgcgacggg catacaaata ctcgttttcg ttaattgagt
    31561 gcccgcccca gcagaccacc atatttggcg cttcaccgac atgcagtgcg cgagcgttac
    31621 gcaagatgga aaagaccagg ttagtgatgt ggacggagtt gtcgagattc aggttgggaa
    31681 aacgaacggt gttatggatt tgcccgtaaa cgaagagaat gtcacgcaga accgcgaaca
    31741 agttggcctg caaagcgcga ataattcgcc catcgacaaa agcctcttcc gggggattta
    31801 tcagttccag ctttacgccg cgttcacggc gcaggacgtt aatatcgaaa ttttcaaaac
    31861 gagacagcaa ttctttgctg ttatcggtca aactaccgga gttcagtacg gcaagtgaac
    31921 agttgcgaaa cagttgatag aggtcgctgc tggcggtgcg tttaagcata tccacttcca
    31981 gctgcgacaa catatccatg gagccaagcg ggctaatatg tgtaatcaag aaaaactcct
    32041 tatgggacga atatcgtcat tccctttgat tacaatagcc ctgcctgatg acctttcaca
    32101 accttctgcg tgaatccagc acgcaaaatt gagaaaaaat ttattgccga accagtcttg
    32161 tggtcgatgg gacaaaatcg ctattactgc gccacgggtt aatgtccaga ccgccacgac
    32221 gggtataacg tgcgtaaacg ctcaatttct ctggctggca gaagcgtaac aggtcattaa
    32281 agatgcgttc cacgcactgt tcgtggaact cgttgtgatg acggaaagaa accaggtaac
    32341 gcagcagttt ttctctgtca atttggcgtc cacgatactg aatttggatc gatccccaat
    32401 ctggttgatg ggtgatcagg caatttgatt tcagcaggtg gctgacgagc gtctcttcta
    32461 ctactttttc gccactggtg gcattctcca gatagtcagt agtgaattca tagttatcga
    32521 tagtgatatc ctggtcatca atgcaagtgc cattgaaatg acctatcggt tggccttcca
    32581 gttcatcaag acgatataac gccacgctaa ccttaccctg agcgcaagtg cttaagtcgc
    32641 gctccagcgt ctggcgcacc tcatcccagt tattaaaacg cgtctggtta aaactgttga
    32701 gatagagctt aaaactcttc gactcaatca gatttgcgct ggtgtaatca agttcaacat
    32761 gaccgacagc gacctgcggc aaacctttcg cattcagcca ggaaagttca tacagcgtcc
    32821 agatatccgt accgtgaaaa ggcaggttat ccgctttcag acccagcggg tcgcgattca
    32881 ggctgcgtgg aacgccttgc agtaggctgg cgtcataggt atcccggtaa tcggttgatt
    32941 ttccaagagt caggcccgca agtgcctgat ggtttgcata agaagacatg tttcaccgtc
    33001 ataaaccaga taaactaaac gacaagtgta tctggtttaa gtgaagagag aaattagtgg
    33061 acgatttgac cgcacaagcc ctgaaagatt ttactgcacg ctactgcgat gcgtggcatg
    33121 aagagcataa aagctggccg ttaagtgagg aactgtacgg cgttccttca ccgtgcatta
    33181 tttcaactac cgaagatgcc gtatactggc aaccccagcc gtttaccggg gaacaaaatg
    33241 taaacgcggt tgaacgcgct tttgatattg tgatacaacc cacgattcat accttttata
    33301 cgactcagtt tgccggggat atgcacgcgc agtttggcga tatcaaactg acattgctgc
    33361 aaacctggag tgaagacgat ttccgtcggg tgcaggaaaa tctgattggt catctggtga
    33421 ctcagaaaag gctaaaactg ccgcccacgc tatttatcgc gacgctggaa gaagagttag
    33481 aggtcatctc ggtgtgtaat ctctccggtg aagtgtgcaa agagacgcta ggaacacgta
    33541 aacggacgca tttagcctca aatcttgcgg aattccttaa tcaacttaag cctcttctgt
    33601 aatccaaata ccccatctgg ttgtgagaga tctcttacag actctgtagg agatcgccag
    33661 gaaataagcg aatacttaaa aagataagaa tcgttattct catttaaaaa caacatgttg
    33721 cttgttaatt cttaactttc atatgaaatt ttccttaagg cattgtctgt aagcgccttg
    33781 taagacttcg cgaaaaagac gattctatct tcgtcgacag ggagtcagac aacgaagtga
    33841 acatcaggat gatgacactt ctgaaggaca caccaggatg gtgtttcagg gaaaggcttc
    33901 tggatgaagc gaagaggatg acgcaggacg cgttaaagga cacctccagg atggagaatg
    33961 agaaccggtc aggatgattc ggtgggtcag gaaggccagg aacacttcag gatgaagtat
    34021 cacatcgggg tggtgtgagc aggaagcaat agttcaggat gaacgattgg ccgcaaggcc
    34081 agaggaaaag ttgtcaagga tgagcaggga gcaacaaaag tagccggaat gctgcgaaac
    34141 gaaccgggag cactgtgaat acagtgctcc ctttttttat tcctgctatc cttcgcggca
    34201 gtttttctta ttgaggttgc tttatgacca ctcatgaccg cgttcgtctc cagttgcagg
    34261 cgcttgaagc gttactgcgt gaacatcagc actggcgaaa cgataaaccc cagccgcatc
    34321 aatttaacag tacccaaccg ttctttatgg acaccatgga accactggag tggttgcagt
    34381 gggtgctgat cccgcgaatg cacgatttgt tagataacaa ccagccgtta ccgggcgctt
    34441 ttgccgttgc accctattat gaaatggcgc tggcgactga tcatccgcaa cgcgcgctta
    34501 ttctggctga gctggaaaaa ctggatgctc tttttgcgga tgatgcgagc taatgctgga
    34561 aatactctat caggatgaat ggctggttgc ggtaaataaa ccctccggct ggctggttca
    34621 ccgcagctgg ctggatcgcg acgagaaagt agtggtcatg caaaccgtgc gtgacctgat
    34681 aggccagcat gtgtttactg ctcatcgtct ggaccgaccc acttctggtg tgttgttgat
    34741 gggattatcc agcgaggccg gacggctgct ggcacaacag tttgaacagc accaaatcca
    34801 gaaacgttac catgcgattg tgcgcggctg gttgatggaa gaagcggtgc tggattatcc
    34861 actggtggaa gaactggaca aaatcgctga taaatttgcc cgcgaagata aaggcccgca
    34921 gccagcagtg acgcattatc gcggtctggc gaccgtagaa atgctggtag cgaccggacg
    34981 ttacccgacc acgcgctacg gcctggtgga actggagccg aaaaccggac gtaagcacca
    35041 gctccgtcgc catcttgccc atttgcgcca cccgattatt ggcgacagca aacatggtga
    35101 tttacgccag aatcgcagcg gtgctgaaca ttttggcctt cagcggttaa tgctgcatgc
    35161 cagtcagttg tcattgacgc atccttttac tggcgagcca ctgactattc acgcgggcct
    35221 ggacgacacc tggatgcagg cgttatcaca atttggctgg cggggtctgc ttcctgaaaa
    35281 tgaaagggtt gagttcagtg cgccatcggg tcaggatgga gagataagtt cctaatccag
    35341 ggagaaaacg gtatggcgga aattggtatt tttgtcggca ctatgtacgg aaattcgctg
    35401 ttagtggcgg aagaagcgga agcaattctg accgcgcagg gccacaaagc aacggtattt
    35461 gaagatcctg aattaagcga ctggctgccc tatcaggata agtatgttct ggtggttacg
    35521 tccacgaccg ggcagggcga ccttcctgat agcattgtgc cgctctttca gggaattaaa
    35581 gataccctgg gcttccagcc gaatctgcgt tatggcgtga ttgcgctcgg cgacagtagt
    35641 tatgtgaatt tctgcaatag tggcaaacag ttcgatgcct tgttgcagga acagagcgct
    35701 cagcgggttg gcgaaatgct gttgattgac gccagtgaga acccggaacc ggaaacggaa
    35761 tcaaatccgt gggtcgaaca ttggggcacg atgttgtcct gatatgttca gcgagcggta
    35821 aatgccgttt tagcggtgct gaatcgaatc ttttcaggca aatgccagta aaaactgctt
    35881 catagcgcgg atttttactg gcgtttgcct ggagtcaagc gatccatttc atactcttct
    35941 ttatttcttc gttttaaccc ttcctttctt gttcttgttt tcatttccgt gaagtggatt
    36001 ccaccgtcca gggctaatgc caaaatcggg cctcattgaa cgcattaatg ttgtgttgtt
    36061 gcacggtgag ccgctatggc gcgcttttta tactgctatt gccagatata aacacgcgcc
    36121 gtattcggcg aacgacctat aaaaacggca aaaaacaccc tacgtcacct ctgatttcct
    36181 ggcgatgtcg cagtccagag tgagcgtggc taacgcgaat tttcaggagt gcaacaatga
    36241 gttctttaag tcaggctgcg agcagtgtgg aaaaacgcac gaatgctcgt tactggatag
    36301 tggtgatgtt gtttatcgtc acatccttca actacggcga ccgcgctacg ctttctattg
    36361 ccggttcgga aatggccaaa gatatcggcc ttgatcccgt gggaatgggc tatgtgttct
    36421 ctgccttctc atgggcgtat gttatcgggc agatccctgg tggctggttg ctggatcgtt
    36481 ttggttcaaa acgcgtctac ttctggtcga tctttatctg gtcgatgttt accttgctgc
    36541 aaggcttcgt cgatatcttt agtggattcg gcattatcgt tgccctgttt acactgcgct
    36601 tcctggtcgg gcttgctgaa gcgccatctt tccctggcaa cagccgcatt gttgcggcct
    36661 ggtttccggc gcaggaaagg ggaacggcgg tgtcgatttt taactccgcc caatacttcg
    36721 caacggttat cttcgcgccg attatgggct ggctaactca tgaagtgggc tggtcacacg
    36781 tcttcttctt tatgggcggt ctgggcattg tcatcagctt tatctggttg aaagtcatcc
    36841 acgagccaaa tcaacatccg ggcgtaaatc agaaagagct ggagtacatc gccgcgggtg
    36901 gcgcgctgat caatatggat cagcaaaaca ccaaagttaa agtgccgttc agcgtgaagt
    36961 gggggcagat caaacagctg ctcgggtcac ggatgatgat cggcgtttat atcggtcagt
    37021 actgtatcaa cgccctgact tactttttca ttacctggtt cccggtttat ctggtgcagg
    37081 cgcgcgggat gtcgattctg aaagcgggct ttgtggcttc cgttccggcg gtttgcggtt
    37141 ttatcggcgg tgtgctgggt gggattattt ccgactggct gatgcgtcgc acgggttcac
    37201 tgaacattgc gcgtaaaaca ccgatcgtga tgggcatgtt gttgtcgatg gtgatggtgt
    37261 tctgcaacta cgtcaacgtt gagtggatga tcatcggctt tatggcgctg gccttcttcg
    37321 gtaaggggat cggtgcgctg ggttgggctg taatggctga tactgcgcca aaagagatca
    37381 gcggtctttc cggtggcctg tttaacatgt ttggtaacat ttctggcatc gtcacgccaa
    37441 tcgcaattgg ttatatcgtt ggcacgactg gctcctttaa tggggcgctg atttatgttg
    37501 gtgttcatgc cttaatcgcg gtgctcagct acctggtgct ggtgggcgat atcaagcgta
    37561 ttgagctgaa acctgtcgcg gggcaataag atgacgacac aatccagtcc tgttattact
    37621 gatatgaaag tcattccggt cgccggacat gacagcatgt tgcttaatat tggtggtgca
    37681 cataacgcat atttcacccg caatattgtg gtactcaccg ataatgccgg gcataccggc
    37741 attggtgaag cgccgggcgg agaggtgatt tatcaaacac tggtcaaggc tattccgatg
    37801 gtgctgggcc aggaagttgc gcgcctgaat aaagtggttc agcaggtgca taaaggtaat
    37861 caggcagccg attttgatac cttcggcaaa ggtgcctgga cttttgaatt gcgcgttaat
    37921 gccgtggcgg cgctggaagc cgccttgctt gacctgttag gtcaggcgct gaatgttccg
    37981 gtctgcgaac tgttagggcc aggcaagcaa cgtgatgcta ttaccgttct cggttatctg
    38041 ttttatatcg gtgatcggac caaaaccgat cttccttatc tggaaaatac gccgggcaac
    38101 catgagtggt atcagttgcg gcatcagaaa gcgatgaaca gcgaagccgt tgtgcgtctg
    38161 gcggaagcct cacaggatcg ctatggcttt aaagatttca aacttaaggg tggcgtgtta
    38221 cctggcgagc aagaaatcga cactgttcgt gcattgaaga aacgcttccc tgatgcgcgg
    38281 attaccgttg atcccaatgg tgcctggctg cttgatgaag ccatttctct gtgcaaaggg
    38341 ctgaatgatg tccttaccta tgcggaagac ccgtgcggcg ctgagcaggg tttctccggt
    38401 cgtgaagtga tggcggagtt tcgacgggcg accggcttgc ccgtcgcgac caacatgatc
    38461 gccaccaact ggcgcgaaat gggtcatgcg gtgatgctca atgcggtaga tattccactt
    38521 gccgatccgc acttctggac gctttccggt gcagtccgcg tggcgcaact ttgcgacgac
    38581 tgggggctga cctggggctg ccattccaat aaccactttg atatctctct ggcgatgttt
    38641 acccatgtgg gtgcggcggc accgggtaat cctaccgcta tcgataccca ctggatttgg
    38701 caggagggcg attgtcgcct gaccaaaaat ccgctggaga ttaaaaacgg aaaaattgcc
    38761 gttcctgatg cgcccggtct gggcgtggga ctggactggg agcaggtaca aaaggcgcat
    38821 gaggcctata aacgtctgcc tgggggtgcg cgtaacgacg cgggcccgat gcagtacctg
    38881 atccccggct ggacctttga ccgtaaacgt cccgttttcg gccgtcattg attttgaaaa
    38941 aggacataaa gatgagttct caattttcga cgcctgttgt tactgaaatg caggtcatcc
    39001 cggtggcggg ccatgacagt atgctgatga atctgagtgg tgcacacgca ccgttcttca
    39061 cgcgtaatat tgtgattatc aaagataatt ctggtcacac cggggtaggg gaaattcccg
    39121 gcggcgagaa aatccgtaaa acgctggaag atgcgattcc gctggtggtg ggtaaaacgc
    39181 tgggtgaata caaaaacgtt ctgacgctgg tgcgtaatac atttgccgat cgtgatgccg
    39241 gcgggcgcgg tttgcagaca tttgacctgc ggaccactat tcatgtggtt accgggatag
    39301 aagcggcaat gctggatctg ctggggcagc atctgggggt taacgtggca tcgctgctgg
    39361 gcgatggtca acagcgtagc gaagtcgaaa tgctcggtta tctgttcttc gtcggtaatc
    39421 gcaaagccac accgctgccg tatcaaagcc agccggatga taaatgtgac tggtatcgcc
    39481 tgcgtcacga agaagcgatg acgccggatg cggtggtgcg cctggcggaa gcggcatatg
    39541 aaaaatatgg cttcaacgat ttcaaactga agggcggtgt actggccggg gaagaagaag
    39601 cagaatctat tgtggcactg gcgcaacgct tcccacaggc gcgtattacc ctcgatccta
    39661 acggtgcctg gtcgctgaac gaagcgatta aaattggtaa atacctgaaa ggttcgctgg
    39721 cttatgcaga agatccgtgt ggtgcggagc aaggtttctc cgggcgtgaa gtgatggcag
    39781 agttccgtcg cgcgactggc ctgccgaccg caaccaatat gatcgccacc gactggcggc
    39841 agatgggcca tacgctctcg ttacaatccg ttgatatccc gctggcggat ccgcatttct
    39901 ggacgatgca aggttcggta cgtgtggcgc aaatgtgcca tgaatttggc ctgacctggg
    39961 gttcacactc taacaaccac ttcgatattt ccctggcgat gtttactcat gtagccgctg
    40021 ctgcaccggg taaaattact gctattgata cgcactggat ctggcaggaa ggcaatcagc
    40081 gtctgaccaa agaaccgttt gagatcaaag gcgggctggt acaggtgccg caaaaaccag
    40141 gattgggcgt agaaatcgat atggatcagg tgatgaaagc ccacgagctg tatcagaaac
    40201 atgggcttgg tgcgcgagac gatgcgatgg gaatgcagta cctgatcccc ggctggacgt
    40261 ttgataacaa gcgcccgtgc atggtgcgtt aagacagaaa accaaaaccg gctctatctg
    40321 gagccggttt gagactgatg ccaaactgcc tgttgcgcta cgtatcaggg ttacatcatt
    40381 ttttaattca ttgaatttaa ttggttttgt aggccggata aggcgttaac gccgcatccg
    40441 gcataaacac aggcactttt caccaatctg aaaccagcgt cataaaaatc cggttgctac
    40501 tcgacaagac gtccattacc cgagaatttt gctggcttcg cgcgctacat tatccatctc
    40561 gtccaacagt tccagcagct ccggctctaa atcttcttct ttagtaccgc tacgtagctg
    40621 ttgttcgatc agttggcaga gattcttcat acgcggtaca ccgctatagc cgcaactgcc
    40681 atgcagttta tgaatcaaat caaccaggcc ttccgggttt tctccaacca gctgttcctc
    40741 aactttgttg cgaacttcag gcaggaaatc gagtaacatt tgcagcatat cgcgcgctaa
    40801 atcggttttt cctgctgcct ggcgtagtgc cagttgccag tcgagggtcg cattcgggtt
    40861 gaccacaatt tcgttgactt cgggcgtcac gacgcgagag gaaataccgc tgccaggctt
    40921 gtagcgcaac agcaaattat gcaatcgctc ttcttcgatc ggtttggcca gataatcact
    40981 catccccgcg ccaagcagtt tctctttttg cccggccatc gcatgcgccg ttaccgcgat
    41041 aaccggcgtt tgctgctgat gcgggagctg gtggatgagc tcacatgccc gaatgccgtc
    41101 catgtccggc atttgaatat ccattaagat taaatcgaac ggcatctgtt tcgcccgttc
    41161 aaccgcctga tgcccgctat cgcaaagttc cacatgttgc accatatctt ccagcaatgc
    41221 gccgataagt ttcaggttag cggggttgtc atcaaccgcc atgactgtca ttgccagctt
    41281 actttcatcg gttacaggca aaagcgtgtt ttgtttgtga tgacaaaatt ccgtcagggc
    41341 aggcaacagg cgcgtaggtg ttaatggttt cagcagacac gcgccgatac catcttgctt
    41401 aagtttttca gcattgactt gtgcatggca aggaagtgcc agcatcagga aatcggtcat
    41461 cgatactgct ttcgctaatc gctcatgttg cattgttagc ggctcgcgga aggtcaccgc
    41521 tatgcctaat aacatcatgt cgtaatgcgc gggaggcagc gcggagaacg ttgggctata
    41581 aaccacttcc agcggcgttt cacttaaaat atccagcgtg cattgtgctg ctgcggagtt
    41641 tggttcgaca taggccaggc gcttaccagc gaggcactgg gtggatggcc cttcgataat
    41701 aatgttcggg ttcagatcga gattaatgtg gaaccagaaa gttgaaccac gattcggcag
    41761 gctatggaac gaaatatcgc cgcccatttc attaaccagt ttttgtgtaa tcaccagccc
    41821 cagcccggtg ccaccatgac ggcgggaaat actggcatca gcctgtctga aggcctggaa
    41881 taagcgcgat tgatcgcgtt caggaatacc aatgccggta tcccgaatct gcacttcaat
    41941 ctgcactttg gtattactca gcgcacgttt ttctaccaga atatcgatgt tgccattctc
    42001 ggtgaattta attgcattcc ctaccaggtt agtgatgatt tgctgtaatc gtagtgggtc
    42061 gccgatcacg ttatcaggca cgtcgctttt aatattgagc gtcagttcca gccctttatc
    42121 gtgagaagaa tgtgccagca gggtaacgac ttcatccagc gtgctgcgta gtgggaatgg
    42181 aatactttcc agaatcagct taccggcttc cagtttcgag aagtcgagaa catcattaat
    42241 aattgccagc aaattatttg ccgaacgttc aatcgtattc aggtgatcgc gctgcgttgg
    42301 tgttaattct gttttcagcg tcaggcgggt aaagccaata acaccattca gtggtgtacg
    42361 cagctcgtgt gacatatttg ccagaaactc ggatttaata cgcgccgctt cctgggcgcg
    42421 ctttttcgcc agatccaact caacgttctg aatctccatt tgctccagcg tctcacgcag
    42481 atcggacgtc gcctggtcga tattgtgttg catctcttcg tgataagccg ccagcgacat
    42541 tgccatcgag ttgataccgt ttttcagcat atccagctcg ccgagcataa atccttccac
    42601 ccggctgtcg agttgcccgc gacggatgcg gtcgacggta ttcaccatgt tgcgaatcgg
    42661 accggttaca tcgcgcatta agcgccagcc aaaaataagc gcaataccga tacaaaacag
    42721 catcatcacg ctggaaataa agatctcttt atattgctgc aagcgaaccg atttaagatc
    42781 cagctccagc gcaatatatc ccagcatatt ttgactattt ttggcatcgc tactgggcga
    42841 ttcgtcgggg gagtaacttt cagaaataat cggcgtgcgg aggatcataa tatcgccgtc
    42901 acgagtgaca gtgagctggc gaggaaacgg cacgttgctg ccgagctgca ttgatgaggg
    42961 atcaagatga aaattggagg tgacaaagag tcggttattt tcatcataaa ccgaaatcgc
    43021 gcgaacaata tcggaatggc gacgatgcag tacgcttatt aactgaccga tagattcgcg
    43081 attttgcagg ctcatgccat attcagtaga aactgcaagc ggctcaataa tgctggctcc
    43141 ggcatcttcc agttgacgct gcaagtcgtt atagcgatgc acaacgaaaa agatactcag
    43201 caataaacca ataaggacgg tcggtgccag gatcagaatc atcatgcgtg cgcgcaggct
    43261 gtagttggtc atggagttcc gttatgggac aattaaggtc acactgttaa attgagaaag
    43321 tctcgccaat ggcgcaattc tactctgcaa aacgacgcac gacgacgcgt cagatcataa
    43381 ctgtttcagt caacgacctc gactcttttg gtcagggcgt ggcacgacat aacggcaaag
    43441 cgttatttat tcctggatta ttgccacagg aaaacgcgga agttactgtt actgaagata
    43501 aaaaacaata cgcccgcgct aaagtcgtgc gccggttaag cgatagcccg gaacgcgaaa
    43561 cgccacgctg tcctcatttt ggcgtatgcg gcggctgtca gcaacaacac gccagcgtgg
    43621 atttacagca gcgaagcaaa agtgcggcac tcgcccgatt aatgaaacac gaagtctctg
    43681 aagtgatcgc cgatgttccc tggggctatc gccgtcgcgc gcgtttaagt ctgaactact
    43741 taccgaaaac acagcaactt cagatggggt ttcgcaaagc gggctccagt gacattgtcg
    43801 acgttaaaca atgtcccatt ttagtgcccc aacttgaagc attgctgccc aaagtcaggg
    43861 catgcctggg cagcttacaa gctatacgcc atcttggtca tgttgaactg gtacaggcaa
    43921 ccagcggcac gctgatgatt ttgcgccata ccgcaccgct aagttcggct gatcgcgaaa
    43981 aactggaacg cttttcgcat tctgaaggcc tggatctgta tctcgccccc gatagtgaga
    44041 tactcgaaac cgtctctggt gagatgccct ggtatgactc aaatgggttg cgcttaactt
    44101 ttagcccgcg cgattttatt caggtcaatg cgggtgtgaa ccagaaaatg gtagcgcgtg
    44161 cgttggaatg gctggatgtg gaacctgaag atcgcgtact ggatctgttc tgcggtatgg
    44221 gcaactttac actgccattg gcgacacaag ctgctagtgt ggtgggtgta gaaggtgttc
    44281 cggcgctggt ggaaaaaggc cagcagaatg cgcgtcttaa tggcttacag aatgtgacgt
    44341 tttatcacga aaatcttgaa gaagatgtca caaagcagcc gtgggcgaaa aacggcttcg
    44401 ataaagtgtt gttggacccg gcgcgagcag gtgccgcagg tgttatgcag caaattataa
    44461 aactggaacc tattcgtata gtttatgtat cctgtaaccc tgcaacgctg gctcgggata
    44521 gcgaagcgtt attaaaagca ggatatacca ttgcgcgact ggcgatgctg gatatgttcc
    44581 cacacacggg acatctggaa tcgatggtac ttttctcgcg cgttaaatag ttacgatttg
    44641 ccgatttcgg caggtctggt ccctaaagga gaggacgatg gttgcggtaa gaagtgcaca
    44701 tatcaataag gctggtgaat ttgatccgga aaaatggatc gcaagtctgg gtattaccag
    44761 ccagaagtcg tgtgagtgct tagccgaaac ctgggcgtat tgtctgcaac agacgcaggg
    44821 gcatccggat gccagtctgt tattgtggcg tggtgttgag atggtggaga tcctctcgac
    44881 attaagtatg gacattgaca cgctgcgggc ggcgctgctg ttccctctgg ctgatgccaa
    44941 cgtagtcagc gaagatgtgc tgcgtgagag cgtcggtaag tcggtcgtta accttattca
    45001 cggcgtgcgt gatatggcgg cgatccgcca gctgaaagcg acgcacactg attctgtttc
    45061 ctccgaacag gtcgataacg ttcgccggat gttattggcg atggtcgatg attttcgctg
    45121 cgtggtcatc aaactggcgg agcgtattgc tcacctgcgc gaagtaaaag atgcgccgga
    45181 agatgaacgc gtactggcgg caaaagagtg caccaatatc tacgcgccgt tggcgaaccg
    45241 tcttgggatt gggcaactga aatgggagct ggaagattac tgcttccgtt atctccaccc
    45301 gaccgaatac aaacgcatcg caaaactgct gcatgaacgc cgtctcgacc gcgaacacta
    45361 tatcgaagag tttgtcggcc atctgcgcgc tgagatgaaa gctgaaggcg ttaaagctga
    45421 agtgtatggc cgtccgaaac acatctacag tatctggcgc aaaatgcaga aaaagaacct
    45481 cgccttcgat gagctgtttg atgtgcgtgc ggtacgtatt gtcgccgagc gtttacagga
    45541 ttgttatgcc gcactgggga tagtgcacac tcactatcgc cacctgccgg atgagtttga
    45601 cgattacgtc gctaacccga aaccaaacgg ttatcagtcc attcataccg tggttctggg
    45661 gccgggtggc aaaaccgttg agatccaaat ccgcaccaaa cagatgcatg aagatgcaga
    45721 gttgggtgtt gctgcgcact ggaaatataa agagggcgcg gctgctggcg gcgcacgttc
    45781 gggacatgaa gaccggattg cctggctgcg taaactgatt gcgtggcagg aagagatggc
    45841 tgattccggc gaaatgctcg acgaagtacg tagtcaggtc tttgacgacc gagtgtacgt
    45901 ctttacgccg aaaggtgatg tcgttgattt gcctgcggga tcaacgccgc tggacttcgc
    45961 ttaccacatc cacagtgatg tcggacaccg ctgcatcggg gcaaaaattg gcgggcgcat
    46021 tgtgccgttc acctaccagc tgcagatggg cgaccagatt gaaattatca cccagaaaca
    46081 gccgaacccc agccgtgact ggttaaaccc aaacctcggt tacgtcacaa ccagccgtgg
    46141 gcgttcgaaa attcacgcct ggttccgtaa acaggaccgt gacaaaaaca ttctggctgg
    46201 gcggcaaatt cttgacgacg agctggaaca tctggggatc agcctgaaag aagcagaaaa
    46261 acatctgctg ccgcgttaca acttcaatga tgtcgacgag ttgctggcgg cgattggtgg
    46321 cggggatatc cgtctcaatc agatggtgaa cttcctgcaa tcgcaattta ataagccgag
    46381 tgccgaagag caggacgccg ccgcgctgaa acagcttcag caaaaaagct acacgccgca
    46441 aaaccgcagt aaagataacg gtcgtgtggt ggtagaaggg gtcggtaacc tgatgcacca
    46501 catcgcgcgc tgctgccagc cgattcctgg agatgagatt gtcggcttca ttacccaggg
    46561 gcgcggtatt tcagtacacc gcgccgattg cgaacaattg gcggaactgc gctcccatgc
    46621 gccagaacgc attgttgacg cggtatgggg cgagagctac tccgccggat attcgctggt
    46681 ggtccgcgtg gtggctaatg atcgtagtgg gttgttacgt gatatcacga ccattctcgc
    46741 caacgagaag gtgaacgtac ttggcgttgc cagccgtagc gacaccaaac agcaactggc
    46801 gaccatcgac atgaccattg agatttacaa cctgcaagtg ctggggcgcg tgctgggtaa
    46861 actcaaccag gtgccggatg ttatcgacgc gcgtcggttg cacgggagtt agtcttacat
    46921 agctggatat ggcgtcagcc gtatccagcg atatctttta ctcgaaggta ttacacctga
    46981 cggacccgca attttcagga cattctaatg aatcaaatcg accgtttgct caccatcatg
    47041 cagcgcctgc gtgacccgga aaacggctgc ccgtgggata aagagcagac atttgccacc
    47101 attgcgcctt acacccttga agaaacctac gaggtgctgg acgccatcgc ccgtgaagat
    47161 tttgacgatc tgcgcggtga actgggcgat ctgctgttcc aggtggtgtt ttacgcacaa
    47221 atggcccagg aagaagggcg ctttgatttt aatgatattt gcgctgcgat tagcgataaa
    47281 ttagaacgcc gtcatccaca tgtttttgct gacggttctg ccggaaacag ccgcgaagtt
    47341 cttgctcgtt gggagcaaat caaaactgaa gagcgcgcgc aaaaagcgca gcattcggcg
    47401 ctggacgata tccctagtag tttacccgcc ttaatgcgcg cacaaaaaat ccagaaacgt
    47461 tgcgccaacg ttggcttcga ctggacgacg cttggcccgg tagtcgataa agtctacgaa
    47521 gagatcgatg aggtgatgta cgaagcgcgg caggttgttg tcgaccaggc taaactggag
    47581 gaggaaatgg gtgacctgct gtttgccaca gtcaatctgg ctcgtcattt agggacaaaa
    47641 gcggaaatcg cattgcaaaa agcgaacgaa aaattcgagc gtcgttttcg cgaagtggag
    47701 cgtattgttg ccgcgcgtgg actggaaatg acaggtgttg acctcgaaac aatggaagaa
    47761 gtctggcaac aggtaaaacg gcaggaaatt gatctctaag ggaattacgt ggtcaagcgc
    47821 gatttgtgtc attttttaaa tgacaagcgc ttgatttgcg tcaaaaacat ttaccccaaa
    47881 ggggctattt tctcactcct gatttcaata gtgcgctggc gaagaggagg gataatgaaa
    47941 gtttgtggca caggtcatgt tcgggtatac tgctttcccg tcctggttat tccatcgtct
    48001 tttcaaccta acttctcagg ttcagcatga caacgaacta tatttttgtg accggcgggg
    48061 tcgtatcctc tctgggtaaa ggcattgccg cagcctccct cgcagccatt cttgaagccc
    48121 gtggcctcaa tgtgaccatc atgaaactgg atccgtacat caacgtcgat ccaggtacta
    48181 tgagcccaat ccaacacggg gaagtgttcg ttactgaaga cggcgctgaa accgacctgg
    48241 acctggggca ctacgagcgt ttcattcgta ccaaaatgag ccgccgcaac aacttcacca
    48301 cgggtcgtat ctactctgac gttctgcgta aagaacgccg cggtgactac ctcggcgcaa
    48361 ccgtgcaggt tattccgcac atcactaacg caatcaaaga gcgcgtgctg gaaggcggcg
    48421 aaggtcatga cgtagtactg gtcgaaatcg gcggtactgt aggtgatatc gaatccctgc
    48481 cgttcctcga agcgattcgc cagatggctg ttgaaattgg ccgtgagcac actctgttta
    48541 tgcacctgac gctggtgccg tacatggcag cgtctggtga agtcaaaacc aaaccgactc
    48601 agcactctgt aaaagagctg ctctccatcg gtatccagcc tgacatcctg atttgtcgtt
    48661 cagatcgcgc cgttccggcg aacgaacgtg cgaagattgc attgttctgt aatgttccgg
    48721 aaaaagcggt tatttctctg aaagacgtcg attccatcta taaaattccg ggcctgttga
    48781 aatctcaggg gctggacgat tatatttgta aacgattcag cttaaactgc ccggaagcaa
    48841 atctgtccga atgggaacag gttatcttcg aagaagcgaa tccggtaagt gaagtcacca
    48901 tcggtatggt cggcaagtac attgaactgc cggatgctta taaatcagtg atcgaagcac
    48961 tgaaacacgg tgggctgaag aatcgtgtca gcgtcaacat caaactgatc gattcacaag
    49021 atgttgaaac gcgcggcgtt gaaatcctta aaggtctgga cgcaatcctc gtacctggcg
    49081 gtttcggcta tcgtggcgta gaaggcatga ttacgaccgc gcgttttgcg cgtgagaaca
    49141 atattcctta tctgggtatt tgcctgggta tgcaggtggc gttaattgat tacgctcgcc
    49201 atgttgccaa catggagaac gccaactcta cggaatttgt gccagactgt aagtacccag
    49261 ttgtggcgct gattaccgag tggcgcgatg aaaacggcaa cgttgaagtt cgtagcgaga
    49321 agagcgatct cggcggtacc atgcgtctcg gcgcacagca gtgccagttg gttgacgata
    49381 gcctggttcg ccagctgtac aatgcgccga caattgttga gcgtcatcgt caccgttacg
    49441 aagtcaacaa catgctgttg aaacagattg aagatgcagg tctgcgcgtt gcgggccgtt
    49501 ccggggatga tcagttggtc gagatcatcg aagttccgaa tcacccgtgg ttcgtggctt
    49561 gccagttcca tccggagttt acttctactc cacgtgatgg tcacccgctg tttgcaggct
    49621 ttgtgaaagc cgccagcgag ttccagaaac gtcaggcgaa gtaagtaaaa aagttagaac
    49681 ggcaacgcgt accctgggta cgcgttgttt gtctggagtt tcagtttaac tagtgacttg
    49741 aggaaaacct aatgtccaaa atcgtaaaaa tcatcggtcg tgaaatcatc gactcccgtg
    49801 gtaacccgac tgttgaagcc gaagtacatc tggagggtgg tttcgtcggt atggcagctg
    49861 ctccgtcagg tgcttctact ggttcccgtg aagctctgga actgcgcgat ggcgacaaat
    49921 cccgtttcct gggtaaaggc gtaaccaaag ctgttgctgc ggtaaacggc ccgatcgctc
    49981 aggcgctgat tggcaaagat gctaaagatc aggctggcat tgacaagatc atgatcgacc
    50041 tggacggcac cgaaaacaaa tccaaattcg gcgcgaacgc aatcctggct gtatctctgg
    50101 ctaacgccaa agctgctgca gctgctaaag gtatgccgct gtacgagcac atcgctgaac
    50161 tgaacggcac tccgggcaaa tactccatgc cggttccgat gatgaacatc atcaacggtg
    50221 gtgagcacgc tgacaacaac gttgatatcc aggaattcat gattcagccg gttggcgcga
    50281 aaactgtgaa agaagccatc cgcatgggtt ctgaagtttt ccatcacctg gcaaaagttc
    50341 tgaaagcgaa aggcatgaac actgcagttg gtgacgaagg tggctatgcg ccgaacctgg
    50401 gttccaacgc tgaagctctg gctgttatcg ctgaagctgt taaagctgct ggttatgaac
    50461 tgggcaaaga catcactctg gcgatggact gcgcagcttc tgaattctac aaagacggta
    50521 aatacgttct ggctggcgaa ggcaacaaag cgttcacctc tgaagaattc actcacttcc
    50581 tggaagaact gaccaaacag tatccgatcg tatctatcga agacggtctg gacgaatctg
    50641 actgggacgg tttcgcatac cagaccaaag ttctgggcga caaaatccag ctggttggtg
    50701 acgacctgtt cgtaaccaac accaagatcc tgaaagaagg tatcgaaaaa ggtatcgcta
    50761 actccatcct gatcaaattc aaccagatcg gttctctgac cgaaactctg gctgcaatca
    50821 agatggcgaa agacgctggc tacactgcag ttatctctca ccgttctggc gaaactgaag
    50881 acgctactat cgctgacctg gctgttggta ctgctgcagg ccagatcaaa actggttcta
    50941 tgagccgttc tgaccgtgtt gctaaataca accagctgat tcgtatcgaa gaagctctgg
    51001 gcgaaaaagc accgtacaac ggtcgtaaag agatcaaagg ccaggcataa gcctgacttt
    51061 atctgattta aaaatgccag ccttcgggct ggcgtttttt atggttgagt taccagcggc
    51121 cggatgcgcc gccaccgcca gaagaaccgc caccgccgct aaacccgccg cctgagcctc
    51181 cagcggaacc gctgttgtct gaatgataac ttctacctga tgctcttttc tgaagtacac
    51241 ataaacaggt aaagaccata aatatggtga aagtaaaaaa gaaggaaaat acagcaatgg
    51301 aaaaagtttt atcggagaac aggaataaat ctaaaagata gattgctgaa atacagacac
    51361 tgcttttaca tgcgcgacaa aaattactgc cttgatgaaa aaaccagaaa gggaagaaca
    51421 tcactgcaaa tacccaaaaa atagcaaagt agtaatgatc gctggaagac gctgattcac
    51481 tttctgaagg attagccgaa tattggtgtt gtgaagtgag ttggttattc aaagcgatta
    51541 tagctaactc taatccctta gctaattttt gttgtttaaa tgcgggtatc atgttgctac
    51601 ggatgatatc tccagccaga gcatcggtta ccttttcttc cagcccataa cctacctgga
    51661 tgcggacagt gcgatccgac caggcaacaa tgatcagtat cccatcattg cgtttggcat
    51721 ctcctaaacg ccaattgtta aaaacccttg tagcatattg ttcaattgtc tcgtctttgg
    51781 ttgtgggaat aattaataca gcaatttgta cttgggtatt attgaaaata gattctaact
    51841 tccgcgtcag ttctttcttt tcttgtgagt ttaagctact cgtaagatcc gtaacttgtt
    51901 gctgtaattg tgggacaata gttggttggg ctgcaacaaa ggaacacacg aatgtgaata
    51961 ttagtatgaa atagcgcatt ataaatgtcc cattaaagct ggataaataa attgacggcg
    52021 gcatcggtaa tttgagattc gttataatta aaaacgaaca cagaaaaaaa taagtataat
    52081 tttggtgaat gtttttgaca tgattaaatc cctttacttt atttatttct gacataatac
    52141 gttactccct tttattatta acgatctgtt tctcattttt ctttaaacct gcctgtaaca
    52201 gaatgttaat aagacatgat tgttgtggca ggatctgcaa taattaacaa ttatccactt
    52261 cacagagaat gctatgcagt acccgattaa cgagatgttc cagaccctgc aaggtgaggg
    52321 ttactttacc ggcgtccccg ccatttttat tcgtttacag ggatgcccgg ttggctgtgc
    52381 ctggtgcgac accaaacaca cctgggaaaa gcttgaggat cgggaagtct cccttttcag
    52441 cattctggcg aagaccaaag agagtgataa gtggggggcg gcgagcagtg aagacttgct
    52501 ggctgtcatt ggtcgccagg gatacaccgc gcggcatgtg gtgattacgg gtggtgagcc
    52561 ttgcattcat gatttgctac cactgactga tctgctcgaa aagaatggtt ttagctgcca
    52621 gattgaaacc agtggtactc atgaggtacg ctgcacaccg aatacctggg ttaccgtatc
    52681 accaaagctg aacatgcgcg gcggctatga agtgttgtca caggcactgg agcgagccaa
    52741 cgaaatcaag catccagtgg ggcgcgtacg tgatattgaa gcactggatg aactactggc
    52801 aacgctgacc gatgataaac cgcgagtcat tgcactgcag ccgattagcc aaaaggatga
    52861 tgccacacgt ttgtgcattg aaacctgcat tgcgcgtaat tggcgtttgt cgatgcaaac
    52921 acataaatat ctaaatattg cctgattaaa catttataag cgttataaat gggtggaacc
    52981 tgtaaagact tctactcatt tatattgttt gtcgcctctg aaaactcatc catcttgccc
    53041 atcccggact aatcattatc attctctaca aattctgtgg cgttaatttt tcgctggagt
    53101 gaaaattatt gcggcgaagt ttggtagatt ttagtttgta tagagttatt ttaaatattt
    53161 agctttttaa tcaatggatt aagtgctctt taacataatg gatgtgttgt ttgtgtgata
    53221 ctgtaaagtt ggtagattgt gactggctta aaaaatcatt aattaataat aggttatgtt
    53281 tagagagttc cccgcgccag cggggataaa ccgagatttc agaggcagcc gccaggccgt
    53341 tgatcgagtt ccccgcgcca gcggggataa accgccattt cggtagcgcc atcaaaatgg
    53401 ttacgcgagt tccccgcgcc agcggggata aaccgcgtcg ttagcggtct ggtgacgtta
    53461 aaggggtgag ttccccgcgc cagcggggat aaaccgttga tcgccgtggc ggtgagatta
    53521 gaaacgcgga gttccccgcg ccagcgggga taaaccggaa gtggcaaagc tgccgtttct
    53581 tttctctatg agttccccgc gcctgcgggg ataaaccacc atataacccg ttatctcttt
    53641 ctcaagtttt tatattagct gtacttgtaa taagcaacat atccacgtaa cacctcatgt
    53701 tcaaaatagt tctccatgcc agagaagttc acaattatcg atacaaaaaa ttaaatttaa
    53761 tcaaagtgtt atttgtatga tactta
//
LOCUS       DAZGQL010000020        52832 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.020, whole genome shotgun sequence.
ACCESSION   DAZGQL010000020 DAZGQL010000000
VERSION     DAZGQL010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 52832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52832
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.020"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            58..987
                     /gene="pfkB"
                     /locus_tag="ACHKIR_001423"
     CDS             58..987
                     /gene="pfkB"
                     /locus_tag="ACHKIR_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416237.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HFO9989177.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPVGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIVDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            1088..1378
                     /gene="ghoS"
                     /locus_tag="ACHKIR_001424"
     CDS             1088..1378
                     /gene="ghoS"
                     /locus_tag="ACHKIR_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFO9989178.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDDG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEIAITTWEEWNSNRK"
     gene            1484..2344
                     /gene="yniA"
                     /locus_tag="ACHKIR_001425"
     CDS             1484..2344
                     /gene="yniA"
                     /locus_tag="ACHKIR_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFO9989179.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNALSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(2385..2921)
                     /gene="yniB"
                     /locus_tag="ACHKIR_001426"
     CDS             complement(2385..2921)
                     /gene="yniB"
                     /locus_tag="ACHKIR_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HFO9989180.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFFLIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            3068..3736
                     /gene="hxpB"
                     /locus_tag="ACHKIR_001427"
     CDS             3068..3736
                     /gene="hxpB"
                     /locus_tag="ACHKIR_001427"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HFO9989181.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVEQVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYIDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            3900..4490
                     /gene="ydjM"
                     /locus_tag="ACHKIR_001428"
     CDS             3900..4490
                     /gene="ydjM"
                     /locus_tag="ACHKIR_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO9989182.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPESWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            4623..6014
                     /gene="tcyP"
                     /locus_tag="ACHKIR_001429"
     CDS             4623..6014
                     /gene="tcyP"
                     /locus_tag="ACHKIR_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HFO9989183.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLTA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGLLLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPMDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHR"
     gene            complement(6061..6324)
                     /gene="cedA"
                     /locus_tag="ACHKIR_001430"
     CDS             complement(6061..6324)
                     /gene="cedA"
                     /locus_tag="ACHKIR_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HFO9989184.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQENEVEE"
     gene            6507..8768
                     /gene="katE"
                     /locus_tag="ACHKIR_001431"
     CDS             6507..8768
                     /gene="katE"
                     /locus_tag="ACHKIR_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HFO9989185.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGSRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSADGSFMDELLTLMAAHR
                     VWSRIPKIDKIPA"
     gene            complement(9026..9784)
                     /gene="chbG"
                     /locus_tag="ACHKIR_001432"
     CDS             complement(9026..9784)
                     /gene="chbG"
                     /locus_tag="ACHKIR_001432"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HFO9989186.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSLDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVAKFAAEEGIALRIDRQPLSNDG
                     DLPANLRSSQGFSSAFYGEEISETLFLQVLDDSSHRGERSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELDVLTSASLKGAIAQRGYRLGCYRDV"
     gene            complement(9797..11149)
                     /gene="celF"
                     /locus_tag="ACHKIR_001433"
                     /gene_synonym="chbF"
     CDS             complement(9797..11149)
                     /gene="celF"
                     /locus_tag="ACHKIR_001433"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFO9989187.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIRDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASGVKNIFDLPFSEGLIRSMNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCTLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(11253..12095)
                     /gene="chbR"
                     /locus_tag="ACHKIR_001434"
     CDS             complement(11253..12095)
                     /gene="chbR"
                     /locus_tag="ACHKIR_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HFO9989188.1"
                     /translation="MMQPVINALEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDIPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            complement(12103..12453)
                     /gene="chbA"
                     /locus_tag="ACHKIR_001435"
     CDS             complement(12103..12453)
                     /gene="chbA"
                     /locus_tag="ACHKIR_001435"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HFO9989189.1"
                     /translation="MMDLDNIPDTQTEVEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKTMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(12504..13862)
                     /gene="chbC"
                     /locus_tag="ACHKIR_001436"
     CDS             complement(12504..13862)
                     /gene="chbC"
                     /locus_tag="ACHKIR_001436"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HFO9989190.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGSFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYAVGANWLGGANIISGIIIGLVVA
                     EMFTFIVRRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWTLNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDIANAL
                     KF"
     gene            complement(13947..14267)
                     /gene="chbB"
                     /locus_tag="ACHKIR_001437"
     CDS             complement(13947..14267)
                     /gene="chbB"
                     /locus_tag="ACHKIR_001437"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HFO9989191.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(14566..14904)
                     /gene="osmE"
                     /locus_tag="ACHKIR_001438"
     CDS             complement(14566..14904)
                     /gene="osmE"
                     /locus_tag="ACHKIR_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HFO9989192.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            15106..15933
                     /gene="nadE"
                     /locus_tag="ACHKIR_001439"
     CDS             15106..15933
                     /gene="nadE"
                     /locus_tag="ACHKIR_001439"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFO9989193.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPEQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            16163..17050
                     /gene="cho"
                     /locus_tag="ACHKIR_001440"
     CDS             16163..17050
                     /gene="cho"
                     /locus_tag="ACHKIR_001440"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HFO9989194.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSINIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQTIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHDDHALRLRQSLERLRVVCW
                     PWKGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATMLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(17010..17585)
                     /gene="ves"
                     /locus_tag="ACHKIR_001441"
     CDS             complement(17010..17585)
                     /gene="ves"
                     /locus_tag="ACHKIR_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HFO9989195.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMFLESTDRFNHTLKPLQPFAFAADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(17788..18273)
                     /gene="spy"
                     /locus_tag="ACHKIR_001442"
     CDS             complement(17788..18273)
                     /gene="spy"
                     /locus_tag="ACHKIR_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone Spy"
                     /protein_id="HFO9989196.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTSAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(18603..19571)
                     /gene="astE"
                     /locus_tag="ACHKIR_001443"
     CDS             complement(18603..19571)
                     /gene="astE"
                     /locus_tag="ACHKIR_001443"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009017 - succinylglutamate desuccinylase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HFO9989197.1"
                     /translation="MDNFLALTLTGKKTVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPRFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(19564..20907)
                     /gene="astB"
                     /locus_tag="ACHKIR_001444"
     CDS             complement(19564..20907)
                     /gene="astB"
                     /locus_tag="ACHKIR_001444"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HFO9989198.1"
                     /translation="MNAWEINFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRLAAKQ
                     GLLKMKALADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAS
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            complement(20904..22382)
                     /gene="astD"
                     /locus_tag="ACHKIR_001445"
     CDS             complement(20904..22382)
                     /gene="astD"
                     /locus_tag="ACHKIR_001445"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043824 - succinylglutamate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HFO9989199.1"
                     /translation="MTLWINGDWVTGQGASRVKRNPVSGEVLWQGNDADAAQVGQACR
                     AARAAFPRWARLSFGDRQVRVERFAGLLESNKAELTAIIARETGKPRWEAATEVMAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGTQGDAFLARLVAVSQRLTPGRWDDEPQPFIGGLISEQAAQQVVTAW
                     QELEAMGGRTLLAPRLLQAGTSLLTPGIIEMTGVTGVPDEEVFGPLLRVWRYDNFDEA
                     IRMANNTRFGLSCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(22379..23413)
                     /gene="astA"
                     /locus_tag="ACHKIR_001446"
     CDS             complement(22379..23413)
                     /gene="astA"
                     /locus_tag="ACHKIR_001446"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008791 - arginine N-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HFO9989200.1"
                     /translation="MMVIRPVERSDVAALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEESETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLAANENYHHFRVVLVRTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(23410..24630)
                     /gene="astC"
                     /locus_tag="ACHKIR_001447"
     CDS             complement(23410..24630)
                     /gene="astC"
                     /locus_tag="ACHKIR_001447"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HFO9989201.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFAYRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPPDIRHAAYNDINSASALIDDSTCAVIVEPIQGEGGVVPASNAFLQGLRELCDRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLATEECASVMTVG
                     THGTTYGGNPLASAVAGKVLDLINTPEMLNGVKQRHDWFVERLNTINHRCGLFSEIRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAAACEHFVSGGSS"
     gene            25076..25882
                     /gene="xthA"
                     /locus_tag="ACHKIR_001448"
     CDS             25076..25882
                     /gene="xthA"
                     /locus_tag="ACHKIR_001448"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFO9989202.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSPLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRENPVLIMGDMNISPGDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMERLMSWGLVDTFRHANPQTANRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            26049..26759
                     /gene="ydjX"
                     /locus_tag="ACHKIR_001449"
     CDS             26049..26759
                     /gene="ydjX"
                     /locus_tag="ACHKIR_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFO9989203.1"
                     /translation="MNAERKFLFACLIFALVIYAFHAFGLFELLTDLPHLLTLIRQSG
                     LFGYSLYILLFIIATLFLLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRSPLSHPKNEG"
     gene            26764..27441
                     /gene="ydjY"
                     /locus_tag="ACHKIR_001450"
     CDS             26764..27441
                     /gene="ydjY"
                     /locus_tag="ACHKIR_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YdjY"
                     /protein_id="HFO9989204.1"
                     /translation="MLQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMGYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            27455..28162
                     /gene="ydjZ"
                     /locus_tag="ACHKIR_001451"
     CDS             27455..28162
                     /gene="ydjZ"
                     /locus_tag="ACHKIR_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFO9989205.1"
                     /translation="MMKMQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLNSMDGFFTRYGKHTILVCRLLPFVPF
                     DPVSYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRNA"
     gene            28162..28710
                     /gene="ynjA"
                     /locus_tag="ACHKIR_001452"
     CDS             28162..28710
                     /gene="ynjA"
                     /locus_tag="ACHKIR_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFO9989206.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SIFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASITPPTVDDALRARLVTHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            28720..29886
                     /gene="ynjB"
                     /locus_tag="ACHKIR_001453"
     CDS             28720..29886
                     /gene="ynjB"
                     /locus_tag="ACHKIR_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO9989207.1"
                     /translation="MRHCVWLLGLLSLFSLATHASDWQEIKNDAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYDINLKIVHLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTDWAETLPNWRYVDTQLPVREDFSVPTEGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALKEAPDDA
                     TFARVTAPLWQFLDALHPYLWREGKDFPPSPARMDALLKANTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFSEGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRKADPAV
                     WGDPSVLDPQKLPDGQRESLQSRMPQDLPPVLAEPHAGWVNALEQEWLHRYGTH"
     gene            29859..31394
                     /gene="ynjC"
                     /locus_tag="ACHKIR_001454"
     CDS             29859..31394
                     /gene="ynjC"
                     /locus_tag="ACHKIR_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFO9989208.1"
                     /translation="MATPLRYALIFLLWAMMAVIYAPLIPAVLTLISPALSLTHWQAL
                     FADPQLPQALLATLVSTTIAAVGALLIALLVIVALWPGPKWQRMCARLPWLLAIPHVA
                     FATSALLLFADGGLLYDYFPYFTPPMDKLGIGLGLTLAVKESAFLLWILAAVLSEKRL
                     LQQLIVLDSLGYSRWQCLNWLLLPSVAPALAMAMLAIVAWSLSVVDVAIILGPGNPPT
                     LAVISWQWLTQGDADQQAKGALASLLLMLLLAAYVLLGYLLWRSWRRTIPRVDGVRKP
                     ATPLLPGITLASFLPLTGVLCVVLLAILADQSTINSEALINSLTMGLTATFIALILIL
                     AWLEWGSSRRHFWLWLPILLPALPLVAGQYTLALWLNLDGSWTAVVWGYLLWVMPWML
                     FILQPAWQRIDSRLILIAQTLGWSRSKIFFYVKCPLMLRPTLIAFAVGFSVSIAQYMP
                     TLWLGAGRFPTLTTEAVALSSGGSNGILAAQALWQLLLPLIIFALTALVAKWVGYVRQ
                     GLR"
     gene            31394..32047
                     /gene="ynjD"
                     /locus_tag="ACHKIR_001455"
     CDS             31394..32047
                     /gene="ynjD"
                     /locus_tag="ACHKIR_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFO9989209.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGGLAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRAQAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            32114..33421
                     /gene="ynjE"
                     /locus_tag="ACHKIR_001456"
     CDS             32114..33421
                     /gene="ynjE"
                     /locus_tag="ACHKIR_001456"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HFO9989210.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSANWLDKMSTEQLNEWIKQHNLKADAPVALY
                     GNDKDVDAVKKRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAAARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDIEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPDQQVSFYCGTGWRAS
                     ETFMYARAMGWNNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(33430..34050)
                     /gene="ynjF"
                     /locus_tag="ACHKIR_001457"
     CDS             complement(33430..34050)
                     /gene="ynjF"
                     /locus_tag="ACHKIR_001457"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFO9989211.1"
                     /translation="MLDRHLHPRIKPLLHQCVKVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALIVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGSET
                     ILLFVLSCLFPACFPWFAWVFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            34137..34544
                     /gene="nudG"
                     /locus_tag="ACHKIR_001458"
     CDS             34137..34544
                     /gene="nudG"
                     /locus_tag="ACHKIR_001458"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HFO9989212.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVEPDES
                     QRQALVRELNEELGIEATVGDYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARPAD"
     gene            complement(34510..34782)
                     /gene="ynjH"
                     /locus_tag="ACHKIR_001459"
     CDS             complement(34510..34782)
                     /gene="ynjH"
                     /locus_tag="ACHKIR_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1496 domain-containing protein"
                     /protein_id="HFO9989213.1"
                     /translation="MSRALFAVVLAFPIIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQQDDKNLSTNPLVWRRVKP"
     gene            35018..36361
                     /gene="gdhA"
                     /locus_tag="ACHKIR_001460"
     CDS             35018..36361
                     /gene="gdhA"
                     /locus_tag="ACHKIR_001460"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFO9989214.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQVQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLSQGVI"
     gene            complement(36438..38393)
                     /gene="topB"
                     /locus_tag="ACHKIR_001461"
     CDS             complement(36438..38393)
                     /gene="topB"
                     /locus_tag="ACHKIR_001461"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFO9989215.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTSVRQFRGIMAPGGGE
                     GKKKDSPRKRAPKKSPPSEEAGNGVII"
     gene            complement(38398..39441)
                     /gene="selD"
                     /locus_tag="ACHKIR_001462"
     CDS             complement(38398..39441)
                     /gene="selD"
                     /locus_tag="ACHKIR_001462"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HFO9989216.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRHACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRELLCDPQTSGGLLLAVMPEAENEVKATAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            complement(39558..40109)
                     /gene="ydjA"
                     /locus_tag="ACHKIR_001463"
     CDS             complement(39558..40109)
                     /gene="ydjA"
                     /locus_tag="ACHKIR_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HFO9989217.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAAMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDNKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            40270..42126
                     /gene="sppA"
                     /locus_tag="ACHKIR_001464"
     CDS             40270..42126
                     /gene="sppA"
                     /locus_tag="ACHKIR_001464"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFO9989218.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKTLTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            42291..43307
                     /gene="ansA"
                     /locus_tag="ACHKIR_001465"
     CDS             42291..43307
                     /gene="ansA"
                     /locus_tag="ACHKIR_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFO9989219.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            43318..43959
                     /gene="pncA"
                     /locus_tag="ACHKIR_001466"
     CDS             43318..43959
                     /gene="pncA"
                     /locus_tag="ACHKIR_001466"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001587135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HFO9989220.1"
                     /translation="MHPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTTLDDWLRDHEIDELIIMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQE"
     gene            complement(44120..44392)
                     /gene="yeaC"
                     /locus_tag="ACHKIR_001467"
     CDS             complement(44120..44392)
                     /gene="yeaC"
                     /locus_tag="ACHKIR_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HFO9989221.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVTLTEEQKENCLQL
                     VMLWQARNNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            complement(44434..44847)
                     /gene="msrB"
                     /locus_tag="ACHKIR_001468"
     CDS             complement(44434..44847)
                     /gene="msrB"
                     /locus_tag="ACHKIR_001468"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFO9989222.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSQTKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            45189..46184
                     /gene="gapA"
                     /locus_tag="ACHKIR_001469"
     CDS             45189..46184
                     /gene="gapA"
                     /locus_tag="ACHKIR_001469"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFO9989223.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            46268..47170
                     /gene="yeaD"
                     /locus_tag="ACHKIR_001470"
     CDS             46268..47170
                     /gene="yeaD"
                     /locus_tag="ACHKIR_001470"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HFO9989224.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGPAAQQGLPAHG
                     FARNLPWTLKSHREDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKACEIDLEAH
                     GEFETTSALHTYFNVGDITKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYL
                     NPQDCSVINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAY
                     ASETQKVTKEKPAHLAQSIRVAKR"
     gene            complement(47221..48075)
                     /gene="yeaE"
                     /locus_tag="ACHKIR_001471"
     CDS             complement(47221..48075)
                     /gene="yeaE"
                     /locus_tag="ACHKIR_001471"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HFO9989225.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYAEGGAEKVVGEALTGLRDNVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLDYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQIPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISAAQVLLAWVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELAMLDKAYPAPK
                     GKTALDMV"
     gene            complement(48165..48911)
                     /gene="mipA"
                     /locus_tag="ACHKIR_001472"
     CDS             complement(48165..48911)
                     /gene="mipA"
                     /locus_tag="ACHKIR_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HFO9989226.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYDGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            49347..51281
                     /gene="yeaG"
                     /locus_tag="ACHKIR_001473"
     CDS             49347..51281
                     /gene="yeaG"
                     /locus_tag="ACHKIR_001473"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011411173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HFO9989227.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            51394..52677
                     /gene="yeaH"
                     /locus_tag="ACHKIR_001474"
     CDS             51394..52677
                     /gene="yeaH"
                     /locus_tag="ACHKIR_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HFO9989228.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKILPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
BASE COUNT        12970 a        13765 c        13098 g        12999 t
ORIGIN      
        1 tttcttcact ttccgctgat tcggtgccag actgaaatca gcctatagga ggaaatgatg
       61 gtacgtatct atacgttgac acttgcgccc tctctcgata gcgcaacaat taccccgcaa
      121 atttatcccg aaggaaagct gcgctgtacc gcaccggtat tcgaacccgg aggcggcggc
      181 atcaacgtcg cccgcgccat tgcccatctt ggaggcagtg ctacagcgat cttcccggtg
      241 ggaggtgcga ccggcgaaca tctggtttca ctgttggcag atgaaaatgt ccccgtcgct
      301 actgtagaag ccaaagactg gacccggcag aatttacacg tacatgtgga agcaagcggt
      361 gagcagtatc gttttgtcat gccaggcgcg gcattaaatg aagatgagtt tcgccagctt
      421 gaagagcaag ttctggaaat tgaatccggt gctatcctgg tcataagcgg aagcctgccg
      481 ccaggtgtga agctggaaaa attaacccaa ctgatttccg ctgcgcaaaa ccaagggatc
      541 cgctgtatcg tcgacagttc tggcgaagcg ttaagtgcag cactggcgat tggtaacatc
      601 gagttggtta agcctaacca aaaagaactc agtgcgctgg tgaatcgcga actcacccag
      661 ccagacgatg tccgcaaagc cgcgcaggaa atcgttaata gcggcaaggc caaacgagtt
      721 gtcgtttccc tgggtccaca aggggcgctg ggcgttgata gtgaaaactg cattcaggtt
      781 gtgccaccac cggtgaaaag ccagagcacc gttggcgctg gtgacagcat ggtcggcgcg
      841 atgacactga aactggcaga aaatgcctct cttgaagaga tggtccgttt tggcgtagct
      901 gcggggagtg cagccacact caatcaggga acacgcctgt gctcccatga cgatacacaa
      961 aaaatttacg cttacctttc ccgctaactg aaacattccc ccagcattgg gggaatcacc
     1021 accaacctgt cggcaacgcg tttctccgac tatgctcaaa agtcatgtga taacaaaggg
     1081 gtgaactatg gccagtggcg atcttgtccg ttatgtcata accgtcatgt tgcacgaaga
     1141 tacattgact gaaattaacg agttgaataa ttacctgact cgcgacggtt ttttgctcac
     1201 catgacggat gatgatggaa atatccatga gctggggact aatacttttg gacttatcag
     1261 tacccaaagt gaagaagaaa ttagagaact tgtttcgggg cttacccaaa gtgcaaccgg
     1321 taaagatcct gaaatagcca tcacgacctg ggaggaatgg aatagcaaca gaaaataaat
     1381 ggtttttggg caataatcag tctgtggtgt gcgttagctc gtgtttttac accgcattct
     1441 tgcgctaacc ttatgatctg gcagacaaca tgggagagac atcatgtggc aggcaatcag
     1501 tcgtcttttg agcgagcagt taggtgaagg cgaaatcgaa ctgcgtaatg aactgcctgg
     1561 cggagaagtc catgccgcat ggcatttgcg ctatgcagga catgactttt tcgtcaaatg
     1621 tgatgaaagg gaactgcttc ccggttttac cgccgaagcc gaccaactgg agttactgtc
     1681 gcgtagtaaa accgtcaccg tgcctaaggt ttgggcagtt ggcgctgacc gtgactacag
     1741 ttttctggtg atggattatc tcccacctcg cccgctggat gcgcatagcg catttattct
     1801 tggtcagcaa attgcgcgtt tacatcaatg gagcgaccaa ccgcaatttg gcctcgattt
     1861 cgataacgcg ctctccacaa ctccccagcc caacacctgg caacgtcgct ggtcaacttt
     1921 ttttgctgaa caacggattg gctggcagct ggaactggcg gcagaaaaag ggatcgcttt
     1981 cggcaatatc gatgccatcg tcgagcatat tcagcagcgt ctggcctcgc atcaaccgca
     2041 gccttctctg ttgcacggcg atttatggtc cggcaactgt gcgctgggtc cggatggccc
     2101 gtacattttc gacccggcct gctactgggg tgaccgagag tgcgacctgg cgatgttacc
     2161 gctgcatact gaacagccgc cacaaatcta tgacggctat cagtcagtat ccccgctacc
     2221 tgccgatttc cttgaacgtc aaccggttta ccaactctac acgctgttaa atcgtgcaag
     2281 gttatttggc ggtcagcatt tggttattgc tcagcagtca ctggatagat tattagcagc
     2341 ataatatggg gtgaggataa tggccgctcc gtgcggcctt ttgattaaat aaaccccagc
     2401 agagaaaaga acacataacc tgcagcaata caaataaccg gcaggatata cagcgagaaa
     2461 aactgcagga aaatagtatg gtgcggcaca acaatacgtg actcaatttg ttcacgcgtt
     2521 agcccctctt cccctttggc tttttccaga atgagttgat cttcaacacc ttcccgcaag
     2581 aagcgcgcct ggcgactcat ccgggcaccg gaatcctgca acgccagtcc gacaaaaatc
     2641 aggaagaaaa tcacccagaa cataacgttc acgccaccat taaaattggg cgtcggggag
     2701 ttataccaga acagattcaa aaaaggcgta ttcgcctgca tcatatcgat catgacatga
     2761 gtaaagtcga gcatgaccgc attaatgcct tcctggtttt cctgccgggt attcataaat
     2821 ttaagcaaag aaatcaatgt agagatcaat gcaggtataa aaatcaccca acccaaaatc
     2881 cttttcaaaa cagcaatgcg tccagcttgt tgatacgtca tgagttctcc ttgattaaga
     2941 cgcgtcgttt cacttagttt acctgtagat atctgttttc gcccattctt taaaggcgat
     3001 atgataggcg cttaatcata agcacggctt aataccttac atataatgct ctaaaggaga
     3061 ggttgtaatg tcaaccccgc gtcagattct tgctgcaatt tttgatatgg atggattact
     3121 tatcgactca gaacctttat gggatcgagc cgaactggat gtgatggcaa gcctgggggt
     3181 ggatatctcc cgtcgtaacg agctgccgga caccttaggt ttacgcatcg atatggtggt
     3241 cgatctttgg tacgcccggc aaccatggaa tgggccaagc cgtcaggaag tagtagaaca
     3301 agttattgct cgtgccattt cactggttga agagacgcgt ccattattac caggtgtgcg
     3361 tgaagccgtt gcgttatgca aagaacaagg tttattggtg gggctggcct ccgcgtcacc
     3421 gctgcatatg ctggaaaaag tattgaccat gtttgattta cgcgacagtt tcgatgccct
     3481 cgcctcggct gaaaaattgc cttacagcaa gccgcatccg caagtatata tcgactgcgc
     3541 agcaaaactg ggggttgacc ctctgacctg cgtggcactg gaagattcgg taaatggcat
     3601 gatcgcctct aaagcagccc gcatgcgttc catcgtcgtt cctgcgccag aagcgcaaaa
     3661 tgatccacgt tttgtattag caaacgtcaa actctcatcg ctgacagaac tcaccgcaaa
     3721 agaccttctc ggttaatgac cagggggcag tgatcacact gcccctggtt ctttatctga
     3781 atttgcattc aactgacgga ttaatcgcca atttaagaga aagagttaca ccgtcaccac
     3841 ttccgtgcac tgtataaaaa ccctatactg tacgtatcga cagtttagtg agttttatca
     3901 tgacggcgga aggtcacctt ctcttttcta ttgcttgtgc ggtatttgcc aaaaatgccg
     3961 agctgacgcc cgtgctggca cagggtgact ggtggcatat tgtcccttcc gcaatcctga
     4021 cgtgtttgtt accggacatc gatcacccaa agtcgtttct tgggcagcga ttaaagtgga
     4081 tatcaaaacc aatcgcccgc gcttttgggc atcgtggttt tacccacagt ctgctggcgg
     4141 tatttgcgct gctggcaacc ttttacctta aggttcctga aagctggttc attccggctg
     4201 atgcgctaca aggaatggtg ctgggttatt tgagccacat acttgccgat atgctgacac
     4261 ccgccggtgt tcccctgctc tggccatgcc gctggcgttt ccgcttgcct atcctggttc
     4321 cccaaaaggg caaccaactg gaacgtttta tctgcatggc attatttgtc tggtcggtat
     4381 ggatgcccca ttcattaccc gaaaacagcg ctgtccgttg gtcatcgcaa atgatcaata
     4441 ccttgcagat ccagtttcat cggcttatta agcatcaggt tgaatactaa aaaggcaaaa
     4501 atcacctttc tggaataagc aattccattt gaatataaga gccagctcac agttctgtta
     4561 atcttgcgcc aacactatga ctgctacgca gtgatagaaa taataagatc aggagaacgg
     4621 ggatgaactt tccattaatt gcgaacatcg tggtgttcgt tgtactgctg tttgcgctgg
     4681 ctcagacccg ccataaacag tggagtctgg cgaaaaaagt gctggtgggt ctggtgatgg
     4741 gtgtggtttt tggcctcgcc ctgcatacca tttatggttc tgacagccag gtacttaaag
     4801 attctgtaca gtggtttaac attgttggta acggctatgt tcaactcctg caaatgatcg
     4861 ttatgccgtt agtctttgcc tctattctga gcgcggttgc ccgtctgcat aacgcatcac
     4921 agttaggcaa aatcagtttt ctgaccatcg gaacgctttt gtttaccaca ctgattgcgg
     4981 cgctggtcgg tgtgctggtc accaacctgt ttggtttgac ggctgaaggt ctggttcagg
     5041 gtggtgcaga aactgcacgt ttgaacgcca tcgaaagtaa ctatgttggt aaagtctctg
     5101 acctgagcgt tccgcagctg gtcttgtcct ttatcccgaa aaacccgttt gccgatttga
     5161 ccggagccaa tccgacgtca attatcagcg tggtaatttt tgccgcattc ctcggcgtag
     5221 ctgcgctgaa gctgctgaag gatgatgcgc cgaaaggtga acgcgtctta accgctatcg
     5281 ataccctgca aagctgggtg atgaaactgg ttcgcctggt catgcagctg accccttacg
     5341 gcgttctggc gctgatgacc aaagtggttg caggttctaa cctgcaagac atcatcaaac
     5401 tgggaagttt cgttgtcgcg tcctacctcg gtcttctgat tatgtttgca gtgcatggcc
     5461 ttctgctggg tattaatggc gtgagcccgc tgaagtactt ccgtaaggta tggccagtac
     5521 tgacgtttgc ctttaccagc cgttccagtg ctgcgtctat cccactgaat gtggaagcac
     5581 aaacgcgtcg actcggtgtt cctgaatcca tcgccagttt cgccgcctct ttcggtgcaa
     5641 ccattggtca gaacggctgt gccggtttgt atccggcgat gctggcggtg atggttgcgc
     5701 ctacggttgg cattaacccg atggacccga tgtggattgc gacgctggtc ggtattgtta
     5761 ccgttagttc cgcaggcgtt gccggtgtcg gtggtggtgc aactttcgcc gcactgattg
     5821 tgctgcctgc gatgggcctg ccggtaacgc tggtggcatt gttaatctcc gttgaaccgc
     5881 ttatcgacat gggtcgtacg gcgctaaacg ttagtggctc gatgaccgct ggcacgctga
     5941 ccagccagtg gctgaagcaa accgataaag ctattctgga tagcgaagac gacgccgaac
     6001 tggcacaccg ttaatcctga tttgttacgt taaacgcctg tcatcccgac aggtgttttt
     6061 ttattcttcg acctcatttt cctgacgaat ctgatttgcc caacgctggg cggattcagg
     6121 cacagtaaac gccggtgagc gcagaaatga ctctcccatc agtacaaacg caacatattt
     6181 gccacgcagc atccagacat cacgaaacga atccatcttt atcgcatgtt ctggcggcgc
     6241 gggttccgtg cgtgggacat agctaataat ctggcggttt tgctggcgga gcggtttctt
     6301 cattactggc ttcactaaac gcatattaaa aatcagaaaa actgtagttt agccgattta
     6361 gcccctgtac gtcccgcttt gcgtgtattt cataacaccg tttccagaat agtctccgaa
     6421 gcgggatctg gctgatggtc tatagttaga gggttttttg accaaaacag cggccctttc
     6481 agtaataatt taaggagacg agttcaatgt cgcaacataa cgaaaagaac ccacatcagc
     6541 accagtcacc actacacgat tccagcgaag cgaaaccggg gatggactca ctggcacctg
     6601 aagacggatc tcatcgtcca gcggctgaac caacaccgcc tggtgcacaa cctaccgccc
     6661 cagggagcct gaaagcccct gatacgcgta acgaaaaact taattctctg gaagacgtac
     6721 gcaaaggcag tgaaaattat gcgctgacca ctaatcaggg cgtgcgcatc gccgacgatc
     6781 aaaactcact gcgtgccggt agccgtggtc caactctgct ggaagatttt attctgcgcg
     6841 agaaaatcac tcactttgac catgagcgca tcccggaacg tattgttcat gcacgcggat
     6901 cagcagctca cggttatttc cagccatata aaagcttaag cgatatcacc aaagcagatt
     6961 tcctctcaga tccgaacaaa atcaccccag tatttgtacg tttctctacc gttcagggtg
     7021 gtgctggctc tgccgatacc gtgcgtgata tccgtggctt tgccaccaag ttctataccg
     7081 aagagggtat ttttgacctc gttggcaata acacgccaat cttctttatc caggatgcgc
     7141 ataaattccc cgattttgtt catgcggtaa aaccagaacc gcactgggca attccacaag
     7201 ggcaaagcgc ccacgatact ttctgggatt atgtttctct gcaacctgaa actctgcaca
     7261 acgtgatgtg ggcgatgtcg gatcgcggga tcccgcgcag ttaccgcacc atggaaggct
     7321 tcggtattca caccttccgc ctgattaatg ccgaagggaa ggcaacgttc gtacgtttcc
     7381 actggaaacc actggcaggt aaagcctcac tcgtttggga tgaagcacaa aaactaaccg
     7441 gacgtgaccc ggacttccac cgccgcgagt tgtgggaagc catcgaagca ggcgattttc
     7501 cggaatacga actgggcttc cagttgattc ctgaggaaga tgaatttaag ttcgacttcg
     7561 accttcttga tccaactaaa cttatcccgg aagaactggt gcccgttcag cgtgtcggca
     7621 agatggtgct caatcgcaat ccggataact tctttgccga aaacgaacaa gcagcattcc
     7681 atccaggtca tattgttcct ggtctggact tcaccaacga tcccctgttg cagggacgtt
     7741 tgttctccta taccgataca caaatcagtc gtcttggtgg gccgaatttc catgagattc
     7801 cgattaaccg cccgacctgc ccttaccata atttccagcg tgacggcatg catcgtatgg
     7861 ggatcgacac taacccggcg aattatgaac cgaactcgat caacgataac tggccgcgcg
     7921 aaacaccgcc ggggccgaaa cgcggcggtt ttgaatcata ccaggagcgc gtggaaggca
     7981 ataaagttcg cgagcgcagc ccatcgtttg gcgaatatta ttcccatccg cgtctgttct
     8041 ggctaagtca gacgccattc gagcagcgcc atattgtcga tggtttcagt tttgagttaa
     8101 gcaaagtcgt tcgtccgtat attcgtgagc gcgttgttga ccagctggca catattgatc
     8161 tcactctggc ccaggcggtg gcgaaaaatc tcggtatcga gctgacggac gaccagctga
     8221 atatcacccc gcctccggac gtcaacggtc tgaaaaagga tccatcctta agtttgtacg
     8281 ccattcctga cggtgatgtg aaaggtcgcg tggtagcgat tttacttaat gatgaagtga
     8341 gatcggcaga ccttctggcc attctcaagg cgctgaaggc caaaggcgtt catgccaaac
     8401 tgctctactc ccgaatgggt gaagtgactg cggatgacgg tacggtgttg cctatagccg
     8461 ctacctttgc cggtgcacct tcgctgacgg tcgatgcggt tattgtccct tgcggcaata
     8521 tcgcggatat cgctgacaac ggcgatgcca actactacct gatggaagcc tacaaacacc
     8581 ttaaaccgat tgcgctggca ggagacgcgc gcaagtttaa agcaacaatc aaggtcgctg
     8641 accagggtga agaagggatt gtggaagctg acagcgccga cggtagtttt atggatgaac
     8701 tgttaacgct gatggcagca caccgcgtgt ggtcacgtat tcctaagatt gacaaaattc
     8761 ctgcctgatg ggaggcggcg cagttgcgcc gcctcaaatg atttacatag tgcgctttgt
     8821 ttatgtcgga tgcggcgtga acgccttatc cggcctacaa aactgtgcaa attcaatata
     8881 ttgcaggaaa aacgtaggcc tgataagcga agcgcatcag gcagttttgc gtttgtcagc
     8941 agtctcaagc ggcgcagtta cgccgccttt gtaggaatta atcgccggat gcaaggttca
     9001 cgccgcatct ggcaaacatc ctcacttaca catcccgata acaccccaac cgataaccac
     9061 gctgagcgat agcacctttc aacgacgctg atgtcaacac atccagctcc gttaaccgcg
     9121 ggaagcaata agcactctgg cgaatcgtat tatcgataaa cgccgggtgg cacatcactt
     9181 ccagagagcg ctcgccgcga tgactggagt catcaagcac ttgtaggaac agtgtctcgc
     9241 taatctcttc accataaaac gcgctactga atccctgcga actgcgcaga ttagccggta
     9301 aatcaccatc gttagatagc ggctgacggt cgatgcgcaa cgcaatcccc tcttcagccg
     9361 caaactttgc cacaatcggg aaaatctgcg ggaacatatg cacatgatga tggctatcaa
     9421 gatgcgtagg tttgcgtcca aataactcaa tgaaacgcaa atactgactg gcaagctcct
     9481 gagtaatttc ttccagcggt aaagcatctt cttctgccaa ctgccagatc cattttccca
     9541 gcacaccatc gcgagttaac cccggcatag ctgtcagtgg cttgcccata gtaaggacaa
     9601 agtgcatccc tatggccaga ctcggttcat caagactcaa ctgcaccgca tggtcaatag
     9661 cctgcccatt caccagcgcc gtcgtcgatg tgacaatccc attgcgacag gcctcgataa
     9721 tgccgtagtt ctgacctttg cttaagccaa aatcatcggc attaacaatc agtaagcgtt
     9781 ccataatcag cctcggttaa tgtgcttttt taagctctgc gatgcagtcg gcaaaatttg
     9841 gcaaccattt ctcgtgcgcc agaatcatct cgcgtgccag cagctcagca tcgcgatcgg
     9901 aatgcaccaa cggactaagg tttagggcca gtaacacatc gttaaactct ccgctaagtg
     9961 cggcgttgct ggcagcaatc tcgaagcctt taatggtgtg aatcagcccc atcactttat
    10021 catcgaaatg cgtaatgcgc ggatgtggcg tcgcgccatc gcgccccagc gtacaggtca
    10081 tttctaccgc ccagtctgcc ggaatattat caatatgccc atgatgcggg atattaacgt
    10141 aatgttcagc ttgcttgtcg ttgtagatag cgttgatcac ttcgcacgct gcatcagagt
    10201 aataagcccc accgcgctgt tccagttctt tcggcttaac tttcaactcc gggtttttat
    10261 atagctcaaa aagttgtttc tcgactttct gtactacctg cgctcgtgcg ccgcctttgt
    10321 agtattcgcc catttcaata gccagcatct ctttttgctt gaagtaataa agcaaataag
    10381 aacacggcaa cagattcata gaacgaatta agccttcgct aaatggcaga tcgaaaatat
    10441 ttttaacgcc agatgctttt aactgcccgg atgccacacc atcaagcaat tcggcaaagc
    10501 gtgatttgcc atttaccagc acatccttaa tgaacaccat atggttgagg ccgaacagat
    10561 cgatagataa atcatcgctg tctttcagca tcagaacatc gcgaataaac atcttcatgc
    10621 cgatcggaat attacacacg ccgataaagc gtttaaatcc ggtatgacga taaacggctt
    10681 cagtgaccat tcccgccggg ttagtgaagt taatcaccca tgcattcgga catagttctt
    10741 cgacatcttt tacgatgtca aaaatcaccg gaatggtacg cagaccttta aacagaccgc
    10801 ccgcaccgtt agtttcctga ccaagataac catgacttaa tggaatacgt tcatccagtt
    10861 cgcgcgccgg taattggcca acgcgcagtt gggtagtaac gaaatcagca tctttcaatg
    10921 cttcgcggcg atccagcgtt ttataaagct tcatcgggac gccagcgtta tcaatcatcc
    10981 gttggcagag atcaaaaata atatccagtt tcgctttacc accttcgaca tccaccagcc
    11041 ataattcgct gaccggcaat tcgtgataac gcttaataaa tccttccagt aactccgggg
    11101 tatagctgct cccgccacca atagtgacga cttttaattt ctggctcata atttctccct
    11161 tcagtacaga atactgatat ctggcatatc tgccccccgg acataaataa tccagcaaca
    11221 ggacagatat gtgaattgtc aggtataacg acttactgat taaattcagt caatttctta
    11281 cgatagctct taggcgtaaa ggatgttaat ttcttaaacg ttttaataaa caagctcggg
    11341 ctactataac cggcctcaaa cgcaatatcc gtcactgaat agttggtcat ttccagttgt
    11401 tttttggcaa aattaatacg gatttcatta ataatctgca ttggcgtttt accatagtat
    11461 cgttgagtcg ctcgcgtcaa atattcctgt gatttggctg acaacgtcac catattctcc
    11521 agcgccgatt cactaaactg ctctttatca tgcatctttt ctaccgtact tttcagccac
    11581 tgcggtatat catcaatcac ctgttcttcg cggtaatgac gtaaacggtt aatgacataa
    11641 aaagtaacca tctcaacaaa ctcttccagc cctgtttcgc ggaaattcaa tgaagaaatc
    11701 actgtttcca cataggtgag aaaagcgtta ttggtccggt atacctgcga agcgacaaag
    11761 caataaggca acaacggcag gtaatgctgc tcaaaaaagc gtttactgat cccaacgttc
    11821 aatatacgcg tggcaccaaa ttcataaaaa ctttgatggt gcgaacctaa cggaataaaa
    11881 acaaaatcgc cccgttccag taacacgcgc ttaccgttaa tctcctggaa ataacgcccg
    11941 gttaatacca gagtaaattc ataatagtcg tgctgatgca gtccgctgat actctcagtt
    12001 ttattgtaga taaacacatg gaaatttttg ccattaaaca actgctgttc tcgggcagtg
    12061 gcaatttcca gcgcgttaat cactggctgc atcatcgact ccttatgcct tcagtttttc
    12121 atgaagctca attaattcag taatcagttc acgcgcaagc atggacgtca ttaaatgatc
    12181 ctgagcgtgg accagcacca gactcacttt catcttacct tcgcccgcat cgccttcaat
    12241 cagtttcgtc tgtaccagat gcgcttcatt caatgccatc cgtgactgat ccatcatggt
    12301 ttttgctgcg gcaaaatcgc cctgcttcgc ctgtttcagt gccgcatacg ccaggctgcg
    12361 cgcttgtccg gagttgatga taagccccat caccacttct tccagctctt caacttccgt
    12421 ttgcgtatcg ggaatattat cgagatccat catacatcgt tcctctctat tcttaccggc
    12481 acgattaccc gtaccggcat cgattaaaat ttcagggcgt tagcgatatc ttcttcgctc
    12541 tcttctttat caatcgcgtt ttgtgctttg ttagccacca caacaaaggg cagataaatt
    12601 aacgttgcga taccaaggtt gaagagtgcg accagcaatg cggcgacgct accgttggtg
    12661 ttaaagaagg ctcccagacc ggttggcatg gtccacggtg caatattggt caccggagga
    12721 ataatgccca tgtagtacgc tgcgagggtg attgccgcca gaatcggttg taccagtaca
    12781 aacgggataa acatcaccgg gttcatgata attggcagac caaacagaat cggttcgtta
    12841 atctggaaga tgccggacgg cagcgccagt tttgccacct gacgataatc agcacggcga
    12901 gaggcgataa agatagccag gatcagacct aaagtcgcac cactgccccc aaggaaaata
    12961 aaggagtcca gcatcggctt ggcccagata tggaaggtct taccggctgc cagcgccgct
    13021 tcgacggaac catattgctg ataggtcgcg atattttcca gcgcccaagg cgtcataatg
    13081 ccgttgtcca gtgcggtcag cgccagcgca ccatgaatac cgaagaacca gagcagtgga
    13141 acaaagatca cataggccca gcccaccacg ctacccaacg atgccagtgg ggttgagatg
    13201 gtatccataa taatctgatg gaagttggtg ccccaggtat tcaacgtcca ggcaataatc
    13261 cccatcacgg aaagaataat aaagccggga attaaagccg agaaggaacg cgatactgaa
    13321 gcaggtacgc tatcgggcaa tttaatgacc caattgcggc ggacaataaa ggtaaacatt
    13381 tctgccacca ccaggccaat aataatcccg gagatgatat tcgccccacc taaccagttt
    13441 gcgccaaccg cataggcctc accgacacta tatggggtga cggtcataaa tgctgcaacg
    13501 gataacaacc cggccgccag cgcatcgact ttacgctctt ctgccagcgc catgccaata
    13561 aagaacggtg ccattaaaga cattatccct aatgttccgt tatatacgtt gccgccaata
    13621 cctttcagac cattaagtgt ttcaatggtt gaggcatcca ggcgaatacc taaggaataa
    13681 aaaaacgacc cctccccaaa gcttagaaaa acgttgttaa ttaatacaaa catggccccc
    13741 gcaagggtta acggcattaa gcgaataaag ccatttttga ttgcattaac gtgtggctgc
    13801 tttcctattt taactgcaaa agggaggagt accttttcaa gcgatgcaat aacattactc
    13861 atagaaaaat acccttaaaa accgcaattt aaatattgcg gtattgattt atgaaataac
    13921 tctttgacgg gaaaatttaa aataaattaa tttgctgcgg cttttttaat cgctgcaacc
    13981 gcagccttaa gcacgcctaa accatcgact ttgccataaa gcagcgagtc aattacttcg
    14041 accggtttgt tgggtaacaa acgctggatt tcgggcaaca tataagcaat ctgcggccct
    14101 aataacacga catcggcatt ctgacctttt tcaccagcca gtgtttccgg aaatgcttca
    14161 ataatgaccg gaacttcata tttttctgcc tgtgcgcgca tttttgatac cagtaaagag
    14221 gtagacatgc ccgcagaaca aaacagataa atgtgtttct tttccataaa actgccctca
    14281 tcgacgatta tctgtcagcc agacactccg caagccttaa cctgcttcca tgctctgggt
    14341 aacttgcgaa accaacatga ctttttttgt ggctgaaagg agtatacggt atagacctga
    14401 gcaatagtat ttctttgacc tgataaattg tctctccttg acctgcggtt atgaccctct
    14461 tcacatttcg ggcaaataat tcgcgaagat aattaagaac tcaggccaaa aaaaaccggc
    14521 gcaatggccg gtttccgttg taactcaagc tttcaggcga agtgattact tcgcagcctg
    14581 tggatcagtg tcgtattcag cacaggtctg ataaccggag ttgatgacat gtccggtatc
    14641 atctaacgcg acaaagtagg tttctgcttt accatcacgt tgacccagga tgtaggtctg
    14701 gcaagtacca cgagcatgga tcatgctcac ttcagacgaa ggtttacccg caatttgtgc
    14761 aacctgcgcc cggctcatgc cttttttgac gtctttcacc acaggctgta caaactggtc
    14821 tttggtacga tcataagccg tacaacccgc cagcatggtt aataccgccg ctgcactcag
    14881 aattcctgcc atattcttgt tcatattccg tcctcttgtt tatcagcgtg ttagataagc
    14941 ctggaataca ttgggcgctt tttcaagccc gtgaacgaaa cggctccgct ttcagaggat
    15001 tcctgtatga cgttttaacc accattcagc ccgctgtcgc ttgtcgtttc agtagcaacg
    15061 ggttagcttt aaggaagttt tgtcttttct gtctggaggg gttcaatgac attgcaacaa
    15121 caaataataa aggcgctggg cgcaaaaccg cagattaatg ctgaagagga aattcgtcgt
    15181 agtgtcgatt ttctgaaaag ctacctgcaa acttatccgt tcattaaatc actggtgctc
    15241 gggatcagcg gcggtcagga ctccacgctt gccggaaagc tgtgccagat ggcgattaat
    15301 gagctgcgcc aggaaactgg caacgaatca ctgcaattta ttgccgtacg cctgccctat
    15361 ggtgttcagg ctgacgaaca agattgccag gatgccattg cctttattca accggatcgc
    15421 gtattaaccg ttaatatcaa gggcgcggta ttggccagcg aacaggcgct gcgcgaagcg
    15481 ggtattgaat taagtgattt tgtccgtggc aatgaaaaag cgcgtgagcg gatgaaagca
    15541 caatatagca ttgcgggtat gaccagcggt gtcgtggtgg gcaccgatca tgcagcagaa
    15601 gccattaccg gattcttcac taaatatggt gacggcggta cggacattaa tccactgtat
    15661 cgtcttaaca aacgtcaggg taaacagtta ctggcggcat taggttgccc ggaacacctt
    15721 tataagaaag cgccaacggc cgatctggaa gatgatcgcc cttctctacc agatgaagtg
    15781 gcactcggcg tgacctatga caatatcgac gactatctgg aagggaaaaa cgtacctgaa
    15841 caggtcgcca gaacaataga gaactggtat ctgaaaactg aacataaacg ccgtccgcca
    15901 attaccgttt tcgatgattt ctggaagaag taataatttg cacatattgg tttgtgcgaa
    15961 aaagagtact ttgttcacgc cggatgcggc gtgaacgcct tattcgcccc acaaaactat
    16021 gcaaattcaa tatattgcat gagtcgaata ggcctgacag gcgtagcacg tctgacggtg
    16081 taacctttgt catcaacccg cctctttttt aatcgctccc cgcctgttac actggataga
    16141 taaccagcat tcggagtcaa ctgtggtacg gcgtttaact tctccgcggc tcgaatttga
    16201 agctgcggca atttatgaat atcccgaaca tttacgttca ttccttaatg acttacccac
    16261 ccgaccaggg gtgtatctgt ttcatggcga aagtgacacc atgccgctct atatcggcaa
    16321 aagcattaac attcgcagcc gcgtcctttc tcatttacgt accccggatg aagccgccat
    16381 gctacggcaa tcccgacgca tcagctggat atgtaccgcc ggtgaaatcg gcgctctact
    16441 ccttgaagcg cgattaatca aagagcagca gccgctgttt aataaacgct tgcgccgcaa
    16501 tcgccagctc tgtgccctgc aattaaatga aaagcgcgtc gatgtggtgt atgccaaaga
    16561 ggtggatttt tcacgagccc ccaacctgtt tggcctgttt gccaataggc gcgcagcttt
    16621 gcaagcattg cagaccatcg ctgatgaaca aaaactttgt tatggtctgt tgggactgga
    16681 gccgttaagt cgcggtcgtg catgttttcg ttcagcgcta aaacgttgcg ccggagcatg
    16741 ctgcggtaaa gagagccatg acgatcatgc gctacgtttg cgccagtctc tggaacgttt
    16801 acgagtagtt tgttggccgt ggaaaggtgc ggtggcgctg aaagaacagc acccggaaat
    16861 gactcaatat catattattc aaaactggct gtggctgggg gcggttaatt cgctggaaga
    16921 agcgacaatg ttaattcgga cacccgccgg gtttgatcac gacggttata aaattctttg
    16981 taagccgctg ctttccggta actatgaaat tactgaactt gatccggcga atgaccagcg
    17041 agccagttga tttcactgaa caacagttta ccttgcggtt gtaaaagccg cagggtgtgc
    17101 ctgccatcga accagcacgc gccctggtcg gtagtgagca gtttatcgcc caattgccac
    17161 gccccgttaa tgacaaacac taccccgcca cgtgagccaa aggtagtaaa cgtacgttcg
    17221 gcaattctga ctttcgcctt acagacatcc aggcgtgtca tgatgttgaa atccatcgac
    17281 atctgccctg ccgtcagttt cgcttttacc acctggtccg ccgcaaaggc aaaaggctgt
    17341 aacggtttta aggtatggtt aaagcggtct gtgctttcaa ggaacatctc gccgccttcc
    17401 agcaacgtca ctatcctttc catacctgga aacaaagaaa actccccatt cgccgcgatg
    17461 gatgcaatgc tggcacgcca gtaaaaatca cgttttgccg gtgggaaagt gcaaatttcg
    17521 cgcgtttcac cggcagcatt tcgccacaga ttcaccgaca ttttacgcat atcaaagtat
    17581 tccatactcg ctcccggccc gttcttttca ttgttatcgt tcaggtaacc gtggatggtt
    17641 atcggcaact cgggtgattt acgttagtgg cgatcaggct ttttttgcat ctgtttgaga
    17701 acaaaaaaga aaaccgccga tcctgtccac cgcattactg caaggtagtg gacaagaccg
    17761 gcggtcttac gttttttggc tgaaagatta ttcagcagtt gcaggcattt taccttttgc
    17821 cgctggacgt tctgtcagac gcttctcaaa attagcatta aattgctttt tctgttccgg
    17881 cgtcaagatg ttgtaaatct tgttctgggt ttccatgtgc gccagcatgt tagctttgcg
    17941 ctgttcttcc atttttgcga tctgcgcttc agcttttgct ttatcgaagg tatcgctggc
    18001 aatgatgtca tgcattgcgc ggcgttcttc cagcggcgga cgtttcatct ggtcacgctg
    18061 gcctttcatg atttcgcgga tctgctgttt ctgcgcgtcg gtcaggttca ggtctttgaa
    18121 catcatgtcc tgatgcggac cgaacttgcc tttgtggtgc atcatcggct tcgcgtcagc
    18181 cggtgctgag gtagtggtgt ctgcggcatg ggccaggtta gccgcgccaa gagccagggt
    18241 agaggcaaca aacagtgcag ttaatttacg catattctat atccttcctt tcagttattt
    18301 attacggctt tcttaagtag cgtgccgtgt tgacgagatt aactttactg actttagcgt
    18361 caattaatca gagcaacggt aaaacaatga aagtgtaaaa aacacttttg cgccaattat
    18421 ggagaaaaaa agaaaatttg atggagagta atgagagaat attacaacac gatgattttg
    18481 cagagattat gaagaactat accggatgac tggtgataaa taaagcaaat aaccaggatt
    18541 aatctgtatt aatttataag aaagtaactt aatacccgca gaatgatttc tgcgggtaag
    18601 tattagctta ttttttcgag cattaatccc gcgcgtaatc ccaacgctac caaaggatta
    18661 gggaataaca catactctac atcatgggtt acggtaaaac gttcctcccc gtcctgcgcc
    18721 agcaatgttc ctttctcaaa cggcataaaa ttcagcgtgt cacttgccat atgcatttcg
    18781 aaggacggcg agtggcgagt aatttgcgaa accacccgat aacggagcgg cggcgttctc
    18841 acgataccga cactctcacc agatagcagc gcagcaattg cgctggcagt gactgcaaac
    18901 tggcgaagat cgttttgtcc aaagggcaac gctttgccga gttccagcgt acaggccagc
    18961 gcgccaaaat gtctggcgct gaaatgggta aacgtaccac caggctcctg atggaacacc
    19021 agtgcctcca gacccgccgc acccagccac gtcagaaatt tctcgtccca gggaatgtcg
    19081 cgttgcggta atacaccgaa ccgcggatgc aaggagccac gaattgcggt atggagatca
    19141 aggtgccagc gcacagattc tttgccctgg tcataaaaat cttccaggca ctgctccagt
    19201 tcgcgcgccc gacaggtttc cccgctttca gcaaatagct gccaacgacc gccaaacatt
    19261 cgattcatat cgctatggca ataacgtttc ccttgcttca gcgcaggagg attcccgagg
    19321 atcaccagta accgccaacg taacgggatc tcaccgtgag atatcgcgcc aagcaacgcg
    19381 tccagcattt ccacaggtgc cgtctcatta ccgtggatcc ccgctgaaat caccagcgcg
    19441 ccttgcggtg gcgttaatgg cgtcagttcc agcacgccat cgcccagcca gcgccagcga
    19501 acgccgttga tttctcgctc ggtgataacc gtttttttac ccgttaaggt cagagcaaga
    19561 aaattatcca tttccccctc cctcgcgctg gaacggataa accgaaccga gattcagtaa
    19621 ttggctcaat acatccagtg cttcccgccc ttcgcgcagc aattgcgggt cggccagatc
    19681 ggcagcagta aggcgatcgc ggtagtaacg atccacccag tcattgagcg cattaaacag
    19741 cgtatcgttc atcatcaccg ccggattcac tgcccggcgt tcttcttctg tcaataccac
    19801 gcgcaaccgc aggcacgccg ggcctccgcc attcgccatg ctttcacgta aatcaaagac
    19861 ttttagttcg ctaatcgggt tgtcagcggc aaggagttca ttgagataac cccatactcc
    19921 ggcgtgttcc cgacactcct gaggcagcac caacatcatg gaaccatcat cgcggctcag
    19981 cagttggctg ttaaacagat atgtagacac cgcatctgac acggaaacct gagtcgccgg
    20041 aacttctatc gccataaaac cgttcacccg cgcacgcagg cttgccagta actgtgactg
    20101 gcgagcgaac gcctgttgat ggcaaaacag cacctggcgg ttactcacgg caatcacgtc
    20161 attatgaaaa acgccctggt cgataacgtc cgggttttgc tgggcgaaaa tcacctgttg
    20221 gggattcacc tgattcagcc ttgccaccgc ctcgctggct tcgcgagtct gccgcgccgg
    20281 ataacgggaa ggccgggtat cattgccttc ttctcgcccg tagacaaaaa gttgcatacc
    20341 cggttcaccg taatgaccgc cgagacgatt gtggtttgcc gccccctcat caccgagcaa
    20401 cgctacctgc ggcaacgccg aatggacact aaatttctct tcgtcgttaa aaatcgcttt
    20461 taacaacgat tcggtgacgg gggcttccag cgaacggtga aatttattgt tcaggttggc
    20521 aacggtgaga tgcactttgc catccagcgt atcggcagat ggcgcgatcg ttgccgcatt
    20581 ggctacccac attggcgaag cggagctgac gctggaaagc cagtgcggtg cctggcgggc
    20641 aaccttttcc agtacctgct catcgctgcc gctgaatccc aactgacgca gcaccggaat
    20701 aaacggacgc tcgtgcggcg ggatcaccgc ctgggggaat cccgcgtcgg caagggcttt
    20761 cattttcagt aagccctgct tcgccgccag tcgcgggtta gagacctgaa aacggtgacg
    20821 ggtggaggct tcattaccaa acgacaggcc cgcgtaatga tgcgtcagcc ctaccagccc
    20881 gtcgaaattg atttcccagg cgttcatcgc accacctcat cggaaaaatc cagcccgggg
    20941 ttaagcgttg cgggcaatgt taacgagtcc gactccagac ttgccatcgg ccaggcacaa
    21001 taatctgcgg cataccaggc gctggggcga tggttaccgg aagcgccaat gccgccgaat
    21061 ggcgcggtac tggcagcacc ggtaagcggt ttgttccagt taacaatccc cgcccgtgcc
    21121 tccagcaaca gttgatcgaa tttttcccgc tcgggggaaa ccagaccgca agagaggccg
    21181 aagcgagtgt tattcgccat tcgaatcgct tcatcgaaat tgtcataacg ccagacgcgc
    21241 aataacggcc caaacacttc ttcatccggc acgccagtaa cgcctgtcat ttcaatgatc
    21301 cccggcgtca gcaacgatgt ccctgcttgt aataagcgcg gcgcaagcag ggtccgtccg
    21361 cccatcgcct ccagttcctg ccaggcagta accacctgct gtgcggcctg ctcagaaatc
    21421 agcccgccaa taaacggctg cggttcgtca tcccaacggc ccggcgttaa ccgctggctg
    21481 acggcaacca ggcgagcaag aaatgcatcg ccctgcgttc cacttttcag caataaacgg
    21541 cgggcgcagg tgcagcgttg cccggcagtg acaaacgccg actgaatggt cagatggaca
    21601 gcggcatcga tatccgccac ctcatcgata attagcgggt tattaccgcc catctcaagg
    21661 gcgagaattt tctccggctg accggaaagc tggcgatgca gctggtagcc ggtattggcg
    21721 ctgccggtaa acagcaagcc gtcgagatcc tccagcgcac tcagcgcctg acccgtttca
    21781 cgcccgccct gcaccaggtt cagcacgccc ggcggcaagc cagcctgctg ccataaacgc
    21841 attaccgctt cgccactcca cggtgtcagt tcgctgggtt taaagataat ggtgttacct
    21901 gccagcaatg ccggaacgat atgtccgttc ggtaaatgac cggggaaatt atacggccca
    21961 aacaccgcca gcacgccgtg cgggcgatgt cgcaggctcg ccgcgccgtc cggcatttca
    22021 ctacgctgct cgccggtacg aacgtgatac gccttaattg atatcgcgat tttattgatc
    22081 atcgccatca cttcggttgc cgcttcccag cgcggcttac ccgtttctct ggcaataatc
    22141 gcggttaatt cggctttatt gctttccagc aatccggcaa agcgttcaac tcttacctga
    22201 cgatcgccaa aggagagacg cgcccagcgc ggaaacgccg cacgggcagc ccgacaagcc
    22261 tgcccgacct gagcggcatc ggcatcattg ccttgccata acacctcgcc cgataccgga
    22321 ttacgcttca cacgcgatgc gccctggccc gtaacccagt caccattgat ccataaagtc
    22381 atgctgtttt ctcctctgcg cacaggcgca ccagacgaac gcgatccccg gcgtggcatt
    22441 tcagggcatc cagttgtgcg gcggttaata tcaaacgctc ggttgccgga tcggtacgca
    22501 ccagcaccac gcggaaatgg tgataatttt cattggcggc caggcaggcc gggaaatcgc
    22561 cctgcgcagg ctgcccttct gccacttcca ccagccgact tttgcggatg gcgcgcacgc
    22621 ggtcgatgtc acactcaagc gtcggcccgc cgtcaaagat gtcgatatag ttgcggtagc
    22681 gaaaaccttc tttctccagc accgcgcggg cgggcgcggt ttgcggatgt acctggccga
    22741 taacgtcctg cgcttcctgg gataaaaagt gggtatagat cggatgtttc ggcatcagtt
    22801 ccgcaataaa tgccttttgc ccggtgccac agagaaaatc ggcgcggcta aaatccatcg
    22861 aaaagaagcg tttaccgagg ctttgccaga acggcgaata gccatgttcg tcaatcaccc
    22921 cgcgcatttc ggcgaccact ttgtcattaa acttgtcgcg aaaggccgcc ataaacataa
    22981 agcgcgattt cgacagcaaa tagccgttgc cctctttgcg ccagtccggg tcgagaaaca
    23041 gcgtgcacag ctcgctgctg ccggtgtgat cgttactgag aaacagcgtc ggcaacgcgt
    23101 tatagacatt cagctctttt gaggcgtgaa ccagcgtgcc gacgcgatag ttgtaccagg
    23161 gatcgttcag cccaaccgcc acctcaatgg cgcaaatccc cgccacagtg cctgtttcgc
    23221 tctcttccag tacaaacaca taaccctgct cgcttttggg cagttcgccc tgccaggttt
    23281 tgatggccct ttcgatacgc gctgaaagcg tggcttcatt agcagggagc gacgtcaggc
    23341 cgccgcccgt tttgctggca agctgcatca gcgccgcaac gtctgagcgc tcaacgggac
    23401 ggatgaccat catgatgaac ctccgctcac aaagtgttca caagccgccg caaaacgatc
    23461 cagcccggtc gtcacctctt cttcgctgac attgagcgct ggcgcaaaac gcactacgtt
    23521 gccaccggca atcagcacca ttacgcccgc tttcgccgct tcctgagaaa tttgtttcgc
    23581 ttgcccggcg taatcggcat tcagcacaca gccaatcagc agacctaatc cgcgaatttc
    23641 actgaacaaa ccgcagcggt gattaatggt attaagacgc tcaacaaacc agtcgtgacg
    23701 ctgtttaacg ccattaagca tctctggcgt gttgatgaga tccagcactt tacctgccac
    23761 cgccgaggcc agcggattac cgccataagt ggtaccatgt gtgcccacgg tcatcacgct
    23821 ggcgcattct tcggttgcca acagagcgcc aacggggaaa ccgccgccca gtgctttggc
    23881 ggtagttaac agatcgggcg tcacgccgta gtgcatatag gcatacagtt ccccggtgcg
    23941 cccgacgccg gtttgtactt catcaaaaat caacagcgca ttgtggcggt cacacaattc
    24001 acgcagacct tgtaaaaatg cgttgcttgc tggcaccaca ccgccttccc cctggatggg
    24061 ttcgacaatc accgcacagg tagagtcgtc aatcagcgcg ctggcagagt taatatcgtt
    24121 atatgcggca tgacgaatat ccggcggcag tggcgcaaaa tcctgtgaat aggctggctg
    24181 accgcccgca ctgacggtaa acagcgtgcg accatgaaac gcatttttaa acgccacgat
    24241 accgctctta tggctgccgt agcggtcgtg agcgaattta cgcgccagtt ttagcgccgc
    24301 ttcgttggct tccgcaccgg agttacaaaa gaagacgcga taggcaaacg tggcgtcgat
    24361 caattttttc gccagtcgca gtaccggctc gttggtgtaa ccgttgccgg tatgccagaa
    24421 cttactcgcc tgttcgttca gcgcctcacg cagttccgga tgcgcatggc ccagcgcgtt
    24481 caccgcaatg ccacccgcga agtcgatata ctctttcccc tgctgatccc acaagcgcga
    24541 accttcgcca cgtaccggta taaacggtgc cggagcgtaa acaggtatca tccattcatc
    24601 aaagttttca cgcgtaattg gctgagacat agcgacctct acagtaaata attcgttatt
    24661 tatatgttaa taataagtaa tgtttgcgct gtaaatgtag attgcagggt tcgtgccagc
    24721 cagtgataaa agtgcataaa cggcggaggc taactggaaa tcaaggagtt ataaccaaac
    24781 catatgcatt taaagtgcat ataaagtgaa tacgtttgcg atgtgggtga ataaaaagaa
    24841 taaaaaacgc aatgttatgc agaagtaaaa tataattctg gaattgtgat cattgacgaa
    24901 atttactgga aattactgcg ccattctgac gcaacgcgca ccaaaagcgg gcattttttg
    24961 cgccatcgtt gacatcatta acaaccatcg atcaaatcac ttaacaacag gcggtacgca
    25021 aagcgaaatt ctgctaccat ccacgcactc tttatctgaa taaatggcag cgactatgaa
    25081 atttgtctct tttaatatca acggcctgcg cgccagacct caccagcttg aagccatcgt
    25141 cgaaaagcac caaccggatg tgattggcct gcaggagaca aaagttcatg acgatatgtt
    25201 tccgctcgaa gaggtggcga agctcggcta caacgtgttt tatcacggac agaaaggcca
    25261 ttatggcgtg gcgctgctga ccaaagagac gccgattgcc gtgcgtcgcg gctttcccgg
    25321 tgacgacgaa gaggcgcaac ggcggattat tatggcggaa atcccctcgc cgctgggcaa
    25381 tgtcaccgtg atcaacggtt acttcccgca gggtgaaagc cgcgaccatc cgataaaatt
    25441 cccggcaaag gcgcagtttt atcagaatct gcaaaactat ctggaaaccg aactcaagcg
    25501 tgaaaatccg gtgctgatta tgggcgatat gaatatcagt cctggcgacc tggatattgg
    25561 cattggcgaa gagaaccgta agcgctggct gcgtaccgga aaatgttctt tccttccgga
    25621 ggaacgcgaa tggatggaga gactgatgag ctggggattg gttgatacct tccgccatgc
    25681 gaatccgcaa acagccaatc gtttctcatg gtttgattac cgctcaaaag gttttgacga
    25741 taaccgtggt ctgcgcatcg acctgcttct cgccagccaa ccgctggcag aatgttgcgt
    25801 agaaaccggc atcgactatg aaatccgcag catggaaaaa ccgtccgatc acgcccccgt
    25861 ctgggcgacc ttccgccgct aatttagctg ttctcctggc tcaaactggg tcaggagaat
    25921 taaccttgag aaaaatcaac aaactgtcag taatgatttg ttgcctgccg tcctttgtta
    25981 taccgtcact gcgtttttag ttgtctgacc acttctctat tatcaagttt gatataggaa
    26041 actccacgat gaacgctgag cgtaaatttc tttttgcctg tcttattttt gcgctggtca
    26101 tttacgcttt ccacgctttc ggtttattcg agctgctcac cgatttacct cacttactga
    26161 cactcattcg ccagagcgga ttgttcggtt atagcctcta tattctgtta ttcatcattg
    26221 ccaccctctt tctgttacca ggaagcatac tggtgatcgc tggaggaata gtttttggcc
    26281 cgctcttagg gacactacta tcattaattg ccgccacgct ggcctcctcg tgctcattcc
    26341 tgctggcgcg ctggctggga cgtgatttac tgctgaaata cgttggccat agccatacct
    26401 ttcaggccat tgaaaaaggc attgcgcgta acggtattga ttttcttatt ctgacccgct
    26461 taatcccgtt gtttccttac aatattcaaa attacgctta cggattaacc acaatcgcct
    26521 tctggcctta tacccttatt tcggcactca caaccctgcc cggtattgtt atttataccg
    26581 tgatggcaag cgatctcgcc aatgaaggca ttacgctgcg ctttatttta caactctgtc
    26641 tggcgggcct ggcactgttt attctcgtcc agctcgcaaa actctacgcc cgacacaaac
    26701 atgtggatct gtctgcttcg cgccgcagcc cactttctca ccccaaaaat gaaggataga
    26761 acgatgttgc aacattattc agtgtcatgg aaaaaaggac tggctgcact ctgcttactg
    26821 gctgttgccg ggcttagcgg ctgcgatcaa aaagagaatg cggctgcaaa agtggaatac
    26881 gacggacttt cgaacagcca accgctgcgt gtcgatgcca ataaccatac ggtaaccatg
    26941 ctggtgcaaa ttaatggtcg tttcctcacc gacgacactc gtcacggtat tgtgtttaaa
    27001 gatggctcca acggacataa atcgctgttt atgggttatg cgaccccgaa agccttttat
    27061 gaagccctga aagaggcagg tggtacaccg ggcgaaaaca tgacgatgga taataaagaa
    27121 acgactcatg tcacaggcag caaactggat atttcggtca actggcaagg ggcggcaaaa
    27181 gcgtattcct tcgatgaagt gattgttgac agtaatggaa agaaactgga catgcgcttt
    27241 ggcggtaatt taacggcagc agaagagaag aaaacgggct gcctggtgtg tctggatagc
    27301 tgcccggtcg gcatcgtcag caatgcaaca tacacttatg gtgcggttga aaaacgaggt
    27361 gaagttaaat tcaaaggcaa tgcctcggtt ctcccggcgg ataacacgct ggcaacggtt
    27421 acctttaaaa tcaccgaata aagcaggata aaggatgatg aagatgcaat cgcgaaaaat
    27481 ctggtactac cgtataaccc tcatcatcct gttgttcgct atgctgctgg catgggcgct
    27541 gcttcccggc gtccatgagt ttatcaatcg cagcgttgcg gcgtttgccg ccgtggacca
    27601 acagggtata gaacgcttta ttcagtctta cggcgcactg gcggcagttg tctcgttctt
    27661 gttgatgatt ttacaggcca ttgctgcacc gctacctgcg tttttgatca cctttgccaa
    27721 tgcgtcgctg tttggcgcgt tctggggcgg cttgctgtcg tggaccagtt cgatggccgg
    27781 cgcggcgctg tgctttttta tcgccagagt gatgggccgc gaagtggtgg aaaagttaac
    27841 gggtaaaacg gtactcaata gtatggacgg cttttttacc cgctatggta aacacaccat
    27901 tctggtatgc cgattattgc cttttgtgcc ttttgatccc gtaagctatg ctgccggttt
    27961 gacttcaata cgttttcgct cgttttttat cgccaccggg cttggtcagt taccagcgac
    28021 tattgtttat tcctgggcgg gcagcatgtt aacaggcggt actttctggt ttgtcaccgg
    28081 actgtttatt ctgtttgccc tgaccgtggt gatttttatg gcgaagaaaa tatggcttga
    28141 gcgccagaag aggaatgcct gatgggttta ccgccgctta gcaaaattcc tttcatttta
    28201 cgtccgcagg cgtggctgca tcgtcgccat tacggcgagg tgctaagccc gattcgctgg
    28261 tgggggcgga tcccgtttat cttttatctg gtgtcgatat ttgtcggctg gctggagcgc
    28321 aaacgctcac cgctggatcc ggtagtgcga tcgcttgtca gcgcgcgcat tgcgcaaatg
    28381 tgcctgtgtg agttttgcgt ggatatcacc agtatgaaag tcgccgagcg cactggcagc
    28441 accgataaac tgctggcagt ggctgactgg cggcaaagcc cgctctttag cgatgaagaa
    28501 cggctggcgc tggagtacgc agaagccgca agcataacgc cgccaacggt cgatgatgcc
    28561 ctgcgcgccc gactggttac gcattttgac gctcaggcgc tcaccgaact gacggcattg
    28621 atcggactgc aaaacctgtc agcccgtttt aattctgcca tggacattcc cgctcagggg
    28681 ctgtgccgta ttcctgaaaa acgttcttaa ggagagatga tgcgccattg tgtgtggttg
    28741 ctgggattgt tatcgctgtt ttctttggca acacatgcca gtgactggca ggaaatcaaa
    28801 aacgacgcca aagggcaaac cgtctggttt aacgcctggg gcggcgatac cgcaattaac
    28861 cgctatctcg actgggttag cggcgagatg aaaacccatt acgatataaa cctgaagatt
    28921 gttcatctcg cggatgccgc agacgcggtg aagcgcattc agaccgaagc tgctgccgga
    28981 cgcaaaacag gcggttcggt ggatctgctc tgggtgaacg gcgaaaactt ccgcacctta
    29041 aaagaggcta atttactgca aaccgactgg gcagagactc tgcccaactg gcgctatgtc
    29101 gacacacagc ttccggtgcg ggaagatttt tcagtgccta cagaaggggc tgaatcgccc
    29161 tggggcggcg cacaactgac gtttatcgcc cgccgcgatg ttacgccaca gccaccacaa
    29221 acgccgcaag ccttactgga gtttgctaaa gccaatcccg gcacggttac ctatccgcgc
    29281 ccaccggact ttaccggcac ggcgtttctt gaacagttgc tgattatgtt gacacccgat
    29341 cccgccgcat taaaagaagc gccggacgat gcgactttcg cccgtgtcac tgcgcccttg
    29401 tggcaatttc ttgatgcgct gcatccgtat ttatggcgcg aaggaaagga tttcccgcct
    29461 tcgcccgcgc ggatggatgc tctgctgaaa gctaacacat tgcgcctgtc gctgaccttt
    29521 aatcctgctc atgcgcagca aaaaatcgcc agcggcgatt tacccgccag cagttacagc
    29581 tttggcttta gcgaggggat gattggcaac gtccatttcg tcaccattcc cgccaacgcg
    29641 aatgccagtg ctgcggcgaa ggtagttgcc aatttcctgc tctcacccga tgcacaactg
    29701 cgtaaagcag atcccgctgt ctggggcgat ccttctgttc tcgatccgca aaaactgcct
    29761 gacgggcagc gcgaatcatt gcaatcgaga atgccgcagg atctgccgcc ggtactggct
    29821 gaaccgcacg caggttgggt aaatgcgctg gaacaagaat ggctacaccg ttacggtacg
    29881 cattaatctt tttgctgtgg gcgatgatgg cggtgattta tgcaccgctg atcccggcag
    29941 ttctcacgct gatatcgcct gctttgtcgt tgacgcactg gcaggcgtta tttgccgatc
    30001 cacagttacc gcaggcatta ctggcaacgc tggtgtcgac aaccattgcg gcggtcgggg
    30061 cgttgttgat tgccctgctg gtgattgtag cgctgtggcc agggccgaaa tggcagcgta
    30121 tgtgcgcccg tctgccgtgg ctgctcgcca ttccccatgt ggcttttgcc accagcgccc
    30181 ttctgctctt tgctgacgga gggctgcttt atgactattt cccgtatttc actccgccaa
    30241 tggacaaatt gggtattggg ctgggcctca ccctggcagt gaaagaaagc gcatttctgc
    30301 tgtggatctt agcggcagta ttgagcgaaa aacggctgtt gcagcaactc attgtgctgg
    30361 attcgctggg ctacagccgc tggcaatgcc tgaactggct gctgttgccc tctgtcgcgc
    30421 ctgcgctggc aatggcgatg ctggcgattg tggcctggtc gctgtcggtc gtggatgtgg
    30481 cgattatcct tgggccaggt aatcccccga cgctggcagt aattagctgg cagtggttaa
    30541 ctcagggcga cgccgatcaa caagcgaaag gcgcactcgc cagcctgctg ctgatgctgt
    30601 tactcgccgc ctacgttttg ctgggctatc tgctatggcg tagctggagg cgtactattc
    30661 cccgcgtaga tggcgttcgt aagcctgcca cgcctttatt gcctggcatt actctggcga
    30721 gttttttacc cttgaccggt gtgctgtgtg tggttctgtt ggcgatcctc gcggatcagt
    30781 cgacgatcaa tagtgaagcg ctcatcaata gcctgacgat gggacttacc gcgacgttta
    30841 tcgctttaat cctgatactg gcgtggctgg aatggggatc gtcacgtcgg catttctggc
    30901 tatggttgcc cattttatta cccgctctgc cactggtggc gggccagtac acgctggcgc
    30961 tatggctaaa cctagatgga agctggacgg cggtggtctg ggggtatctg ctgtgggtaa
    31021 tgccgtggat gctgtttatc ctgcaacccg cctggcagcg cattgattca cggttaattt
    31081 tgattgcgca aacactgggc tggtcgcggt ccaaaatctt cttttacgtg aaatgtccgc
    31141 ttatgctacg ccctacgctg attgccttcg cggtgggatt ttcagtcagt attgcacagt
    31201 atatgccgac gctgtggctg ggcgcggggc gttttccgac gctcaccact gaagcggtgg
    31261 cattaagcag cggcggcagc aacggcattc tcgccgccca ggctttatgg caactgctat
    31321 taccgcttat tatttttgcc ctgaccgcgt tagtcgcaaa atgggtaggt tatgtcagac
    31381 aaggactccg ctaatgctct gcgtgaaaaa tgtttcgcta cgtttaccag aaagccgctt
    31441 gctgacaaac gttaacttta cggtggataa aggtgacatt gtcacgttaa tggggccgtc
    31501 tggctgtgga aaatccactc tgttttcatg gatgattggt ggactggccg gacagttttc
    31561 ttgtacaggg gagctatggc tcaatgagca acggattgac atgctaccca ccgcacagcg
    31621 tcagattggc attcttttcc aggatgcact gttatttgac cagttcagcg tcgggcaaaa
    31681 tttactgctg gcgctaccgg cgacacttaa agggaatgcc cgacgtaatg ccgtgaatga
    31741 tgcacttgag cgttcaggcc ttgacggagc tttccatcag gatcctgcta ctttgtctgg
    31801 cggtcagcga gcgcgcgttg ctctgctacg cgcccttctc gcccagccaa aagcgttact
    31861 cctggatgag ccattcagcc gtctagatgt ggctctgcgc gataattttc gccagtgggt
    31921 gttcagcgaa gttcgcgccc aggcgatccc cgtcgttcag gtaacgcacg atctccagga
    31981 tgttcctgct gatagttctg ttctggatat ggcgcagtgg tcagaaaatt acaacaaact
    32041 gcgataacgc aaagtttttc tcaatgcgtc agttcagaat ggcgcactca aaactacaat
    32101 gtcgggattt tcgatgaaac gtgtttctca aatgaccgcg ctggcaatgg ctttagggct
    32161 ggcttgcgct tcttcgtggg ccgctgaact ggcgaagcct cttacacttg accagcttca
    32221 acaacaaaat ggcaaagcga tagatactcg ccccagcgcg ttttataacg gctggccaca
    32281 aaccttaaat ggcccttctg gtcatgaacc tgccgcctta aacctctctg ctaactggct
    32341 cgacaaaatg agcaccgaac agctcaacga gtggatcaag caacataacc tgaaagccga
    32401 tgctccggtg gcgctgtacg gtaatgacaa agatgtcgac gccgtcaaaa agcgactgca
    32461 aaaagcaggt tttacgcata tctccattct gagtgacgcg ctaagcgaac cttcccgtct
    32521 gcaaaaactg ccgcattttg agcagctggt ttatccgcaa tggctgcacg acctgcaaca
    32581 aggtaaagag gttacggcga aacctgccgg tgactggaaa gtcattgaag cggcctgggg
    32641 cgctcctaag ctttacctta tcagccatat tcccggcgct gactacatcg ataccaacga
    32701 agtggaaagc gaaccgctgt ggaacaaagt ttctgatgaa caactaaaag cgatgctggc
    32761 aaaacacggc attcgccatg acaccacggt cattctgtac gggcgtgacg tatacgctgc
    32821 agcgcgtgtg gcacaaatta tgctctacgc tggcgtgaaa gatgtccgcc tgctggacgg
    32881 cggctggcaa acctggtccg acgcgggact gcccgttgag cgcggaacgc caccgaaagt
    32941 gaaagcggaa ccggatttcg gcgtgaagat cccggcacaa ccgcaactga tgcttgatat
    33001 tgaacaagcg cgtggactgc tgcatcgcca ggatgcatcg ctggtgagca ttcgttcgtg
    33061 gccagaattt atcggtacga ccagcggtta cagctatatt aaaccaaaag gtgaaatagc
    33121 cggagcacgt tggggacacg ctggtagcga ctcgacgcat atggaagatt tccataaccc
    33181 ggatggcacc atgcgtagcg ccgatgatat taccgctatg tggaaagcat ggaatatcaa
    33241 gccagatcag caagtttcat tctactgcgg caccggctgg cgcgcgtcag aaacctttat
    33301 gtacgcacgc gcaatgggct ggaataacgt ctccgtttat gacggtggct ggtacgaatg
    33361 gagcagcgat ccaaaaaatc cggtagcaac cggtgaacgc ggcccggaca gcagtaaata
    33421 acattgcaat tactgacgct ggagcgactt cagcgtcaga tacccactcc acacccgcgt
    33481 aaaggtcgtc atccagcaca acgcaccaaa tacccacgca aaccacggaa aacaggccgg
    33541 aaagaggcaa ctcagaacaa acagcaaaat cgtctcgctg ccttcggtta agccgcccag
    33601 ataataaaac gatttatgcg cataaccggg attatcaatc tgatgtttcg ccgccagcgc
    33661 agcaaaagcg agaaaactgc tgcccgtgcc gataaacgca aacaacagcc agcctccggc
    33721 cagcgcgttt tgttctggtg cggcgagaat aaaaccaaac ggcaccagcg cgtaaaagag
    33781 aaaatcgaga gaaatatcga gaaagccgcc cgcgtcggta agtcctctgc gccgcgccag
    33841 cgcaccgtcc agaccatcaa gcagcctgtt caacaaaatg acgattagcg ccgccagata
    33901 ccagcccagc gccagaaacg gcagtgccag cacgccgatg gcaaatccca ctaatgttaa
    33961 accatctggc gtgatgccgg gtttatccag caccttcaca cactgatgca acaaaggttt
    34021 aattcgggga tgaagatggc ggtctagcac aggcactcct taaatataaa gcctttccga
    34081 ttgagcaaca gtgcggatat tatggcattt ttcgcttatc tgcccgtgtg taatttatga
    34141 aaatgattga agttgttgcc gccatcattg aacgtgatgg caaaatttta ctcgcgcaac
    34201 gccccgccca gagcgatcag gcgggattat gggagtttgc cggtggtaaa gtcgagccgg
    34261 atgaaagcca gcggcaggcg ctggtgcgtg agttaaacga agaactgggc atcgaagcaa
    34321 ctgttggtga ctatgttgcc agccatcagc gagaagtttc ggggcggatt atccatcttc
    34381 atgcctggca cgtacccgac ttccacggga cgttacaggc acatgaacat caggcgctgg
    34441 tctggtgctc acctgaagag gcgctgcaat atccgctggc ccctgctgac attccattat
    34501 tagaggcgtt tatggcttta cgcgccgcca gaccagcgga ttagtgctaa ggtttttgtc
    34561 atcctgttgg cattgcagca gtattccttc ggctttaatt accgcccctt cagaataatt
    34621 ttgatcctga taaacgcagc actgagtaca gggctgcgct gactgcccgc ctgaactgaa
    34681 cacttctggc ggtacgttta cctccacgtc cggacgataa tgcgggttag ccagtgcaat
    34741 tattggaaat gctaatacta cggcgaacaa tgctcgactc acagggaact ccttaacgtt
    34801 attgtctctg ctactgataa cggtagccgg gtggcaaaac tttagcgtct gagttatcgc
    34861 atttggttat gagattactc tcgttattaa tttgctttcc tgggtcattt ttttcttgct
    34921 taccgtcaca ttcttgatgg tatagtcgaa aactgcaaaa gcacatgaca taaacaacat
    34981 aagcataatc gtattaatat ataagggttt tatatctatg gatcagacat attctctgga
    35041 gtcattcctc aaccatgtcc aaaagcgcga cccgaatcaa accgagttcg cgcaagccgt
    35101 tcgtgaagta atgaccacac tctggccttt tcttgaacaa aatcccaaat atcgtcagat
    35161 gtcattactg gagcgtctgg ttgaaccgga gcgcgtgatc caatttcgcg tagtatgggt
    35221 tgatgatcgc aaccaggtgc aggtcaaccg tgcatggcgt gtgcagttca gctctgccat
    35281 cggcccgtac aaaggcggta tgcgcttcca tccgtcagtt aacctctcca ttctcaaatt
    35341 cctcggcttc gaacaaacct tcaaaaatgc cctgactact ctaccgatgg gcggtggtaa
    35401 aggcggcagc gatttcgacc cgaaagggaa aagcgaaggc gaagttatgc gtttttgcca
    35461 ggcgctgatg actgaactgt atcgccacct gggcgcggat actgacgttc cggcaggtga
    35521 tatcggcgtt ggtggtcgtg aagtcggctt tatggcgggg atgatgaaaa agctctccaa
    35581 caataccgcc tgcgtcttca ccggtaaggg cctttcattt ggcggcagtc ttattcgccc
    35641 ggaagctacc ggctacggtc tggtttattt caccgaagca atgctaaaac gccacggtat
    35701 gggttttgaa gggatgcgag tttccgtttc tggctccggc aacgtcgccc agtacgctat
    35761 cgaaaaagcg atggaatttg gtgctcgtgt gatcactgcg tcagactcca gcggcactgt
    35821 agttgatgaa agcggattca cgaaagagaa actggcacgt cttatcgaaa tcaaagccag
    35881 ccgcgatggt cgagtggctg attacgccaa agaatttggt ctggtctatc tcgaaggcca
    35941 acaaccgtgg tctgtaccgg ttgatatcgc cctgccttgc gccacccaga acgaactgga
    36001 tgttgacgcc gcgcatcagc ttatcgccaa tggcgttaaa gccgtcgccg aaggggcaaa
    36061 tatgccgacc accatcgaag cgactgaact gttccagcag gcaggcgtac tgtttgcacc
    36121 gggtaaagcg gctaatgctg gcggtgtagc aacatcgggc ctggaaatgg cgcaaaacgc
    36181 tgcgcgcctg ggctggaaag ccgagaaagt tgacgcacgt ttgcatcaca tcatgctgga
    36241 tatccaccat gcctgtgttg agcatggtgg tgaaggtgag caaaccaact acgtgcaggg
    36301 ggcgaacatt gccggttttg tgaaggttgc ggatgcgatg ttaagccagg gcgtgattta
    36361 agacaattgt gccggataca gcgcaaacgc cttatccggc cataaaaatc ttcagtatca
    36421 gatggttgaa agaaccttta tattatcact ccattgcccg cctcttctga tggcgggctt
    36481 tttttcggcg ctctcttgcg tggcgaatcc tttttcttcc cttctccacc gccgggagcc
    36541 attattcccc gaaactgcct taccgacgtg cgctttgcct gatcaatcaa ctggtacaat
    36601 gtccccacca gcggctgcat aaagtcctga tagcgacact gcttttcgct gatttgcgtc
    36661 agcaccgatt cccagtgcgc ggtcatgtcc ggtcgcgtcg ccatttctgg cagcgaatgg
    36721 aatagcgctt ttccggcgtc ggtggagtgg atatagcgcc cttttttggt caggaagcct
    36781 cgcttgaaca gcagttcaat aatcccggcg cgagtcgctt ctgtccccag accatcggtc
    36841 gcacgaagga tctttttcag atctttgtcc tgcacaaaac gtgctatccc ggtcatcgct
    36901 gaaagcagtg ttgcatcggt aaaatggcgc ggcggctggg tttgccgctc taccacttca
    36961 cctttttcac acagcaactc atcgcctttc gccaccacag gcagcggcgt gccgtcgttt
    37021 tcttcatcgc gctctttgct gcctaacagt gtgcgccagc ctgcttcagc aagaaaacgg
    37081 gctttagcga caaatttgcc tttggcaatg tccagttcga taacacactt gcggaacacc
    37141 gcatccgggc agaactgcat caaatactga cgggcaatca ggttatagac cttcgcttcg
    37201 ttctccgtca ggttgatcgc agaactccgt gcggtcggga tgatggcgtg gtgcgcatcg
    37261 acttttttgt catcccaaca gcggttgcgt atatctggat cgaccactgg ctgcggcaac
    37321 agatctggtg catgaacgct gatagcattc atcaccgcgt ggcgtccggc aaaatgttct
    37381 tctggcaaat agcggcaatc ggaacgcgga taagtgatta gcttgtgcgt ttcgtacagc
    37441 ttctggcaga tatcaagcac gttctgcgcg ctcagaccaa aacgtttagc cgcttcaatc
    37501 tgcaacgccg aaagcgaaaa aggcagcggc gcggattctg attcccgttt atcgttatag
    37561 ctggtgacaa tagctggttg accactaatg cggttaacca catgctccgc cagtggacga
    37621 tgtaataagc gcccttcttc atcctggtac ggttcacacg cttcgctcgg ttgccagata
    37681 gcggtaaacc gctcatcggc aggtgtcacg atatgtgctt tgacttcaaa gaagtctttc
    37741 gccacgaagt tttcaatctc ttcgtcgcgg cgcaccacca gtccgagtac gggcgtctgc
    37801 acgcgtccca cggaaagcac gccctgataa ccggcattgc gaccgagaat ggtatacgca
    37861 cgggtcatat taatgccgta cagccagtca gctcgcgcac gcgccagtgc agaaacgcac
    37921 agcggtacaa actcgctatt ggagcgaaga cgatcgatcg cccgctcaac cgcctgcggg
    37981 ttcaggtcgt ttatcaaaca acgctgtacc tgctggcgct tttccggtgc cagttgcagg
    38041 tagtccagca cttcatccac cagcagttgc ccttcgcgat ccgggtctcc ggcgtgaacg
    38101 atttcgctgg cttcatgcag gaagcgtttg atgacattaa gttgtttagt cacggagggt
    38161 cggggctgta attgccactt ttccgggaca atcggcaaat ccgcaagatt ccagcgcgca
    38221 tagcggctgt cgtaggcgtc tggctgcgcc tgctcaagca ggtgaccgat acaccaggtc
    38281 accacctgac cattaccgca ctcgataaag ccatcgcctt tccggtgcgg tttaggcagg
    38341 acatcagcaa tggcgcgcgc cagactcggt ttttcggcaa taaacaaccg cattgaatta
    38401 acggatctca accatggcac gaccgccgcg cgctggcacc agttcgccaa tcgccgtcag
    38461 ttcaatgcca aactcggcgg ctgtagcttt gacctcattt tctgcttccg gcatgaccgc
    38521 cagcagcaaa ccgccagaag tttgcggatc gcacagcagt tcgcgcactt cacgcggcat
    38581 ttcacccatc agatgaccgt agctggcaaa gttacgttcg gtgccgccag gcactgcgcc
    38641 caacttaatg tactcttcaa cccccggcaa tttcgggatc gcgtcatagt cgacgcgcgc
    38701 ctgcacacct gcgccctgac acatttcgct taaatgcccc agtaaaccaa aacccgtaac
    38761 gtcggtcatc gcttttacgc cttcgatgtt ggcaaaggac gcgcctgcga tgttcatccg
    38821 gcacatcact tccgtcgcca gtccctgatg ttctggtttc agcagtgatt ttttctcagc
    38881 cgtggtaaga acgccaatcc ccagaggttt cgtcaggaac agtttgcatc cggcttgtgc
    38941 ggtactgttt ttcttcaccc gctcggtagg tacgatcccc gttaccgcca gaccaaaaat
    39001 cggctctggc gcatcgatgg agtgaccgcc agccagcgca atccccgcct gacggcatgc
    39061 atggcgtcca ccttcggtca cttcgcgggc aatttccggg gaaagtttgt taatcggcca
    39121 gccgaggatc gcaatcgcca taatcggttt gccgcccatt gcgaagatat cgctgatggc
    39181 gttagtcgcc gcaatgcggc caaaatcgaa aggattatca acgatcggca taaagaagtc
    39241 ggtggtactg ataacgctgg tgccattgcc cagatcgtat accgccgcat cgtcgcgggt
    39301 ttcattaccc acaagcaaat tcggatcaac aaacttcgcc tgctcactgt gcaggatggt
    39361 ttccaacact tttggggaaa ttttacagcc gcaaccagcg ccgtggctgt attgggtcaa
    39421 acgaatcgag ttctcgctca tggacatctc ctgtcaatgc aatccgggta tggtaaccct
    39481 cattccatga agtgataagt gagagtgtct gaattcctgc gcctttgctc acaatccaga
    39541 cagtttcgcg acaattatca gaaataagtc acaaacggcg tcgggtccgg gacgtttatc
    39601 gacgtagatg ctttcaactg cggcgtaccg aggtagagaa aaccgacaat tttatcctgc
    39661 tcacggcaac cgaatgcttc acgcactacc ggactttcag ttaatgcacc actgcgccag
    39721 atgccgccaa acccctgggc aactgctgcc atttgcatcg ccatgaccgc gcatcccgca
    39781 gacatttcct gttcccagcg cgggacttta tgattttctt cgcatttcgc caccaccgtt
    39841 atgatgagcg gtgcgcggaa cggcgcatta cgcgctttgt cgatggcttt gttatcacta
    39901 ccggcagcaa tcgccccctg ctccagtacg gcgctgaaac gctcgcgccc ttccccttca
    39961 atcacaaaga aatgccacgg ttgcatggac ttatggtccg gcgcacgcat agccgcacgc
    40021 aggatgtttt gcagttgttc acccgttggc gcaggttcag ccaggcggga ggcgctacgg
    40081 cgattgatca atagttcgag tgcatccatt tgattaactc ctgtcatgat atttattcac
    40141 aaaattaaca cgagagtgga ttttgttaca gcccagtccg caattcctgc tgacaagtgc
    40201 cggttgggtc attacgataa ccacatctat tgcgcctgaa acaggtgtga ccttaagttg
    40261 ggagaataca tgcgaaccct ttggcgattt attgccggat tttttaaatg gacgtggcgt
    40321 ctgctgaatt tcgtccgtga aatggtactt aacctgttct ttatttttct cgtgctggtt
    40381 ggtgtgggga tctggatgca ggtcagtggt ggtgattcga aagaaacggc cagtcgtggc
    40441 gcactgctgc tggacatttc tggtgtgatc gtcgataaac ccgacagttc tcagcggttt
    40501 agtaaattaa gccgccagct gcttggtgcc agttccgatc gtctgcagga aaactcactg
    40561 tttgatatcg tcaacaccat tcgccaggcg aaggacgacc gcaatatcac cggtattgtg
    40621 atggatctga aaaacttcgc aggcggcgac cagccgtcta tgcagtacat cggcaaagct
    40681 ctgaaagagt ttcgtgatag cgggaaaccg gtttatgccg ttggcgagaa ctacagccag
    40741 gggcaatatt atctcgccag tttcgccaat aaaatttggc tgtctccgca aggcgtggtt
    40801 gatctgcacg gctttgccac caacggtctg tactacaaat cattactgga taagctgaaa
    40861 gtttccaccc atgtgttccg cgtgggtacg tataaatctg ccgttgaacc gtttattcgt
    40921 gatgatatgt cgccggcagc ccgcgaagct gacagccgct ggattggtga gctgtggcaa
    40981 aactatctga atactgttgc cgctaaccgg cagatccctg ctcagcaggt attccctggc
    41041 gcgcaagggt tgcttgaggg tttaaccaaa accggtggcg ataccgcgaa atatgcgctg
    41101 gaaaacaagc tggtcgatgc actggcatcg agtgccgaaa tcgaaaaaac tctgaccaaa
    41161 gagttcggct ggagcaagac tgataaaaat tatcgcgcca tcagttatta cgattacgca
    41221 ttgaaaacgc cggcagatac cggtgacagc atcggagtcg tcttcgctaa tggcgcaatt
    41281 atggatggcg aggaaactca ggggaatgtt ggcggtgata ccactgcggc acaaatccgc
    41341 gacgctcgcc ttgacccgaa agtgaaagcg attgtcctgc gtgttaatag cccaggcggc
    41401 agcgttaccg cgtctgaagt gattcgcgct gaactggcag cagcccgggc agcgggtaag
    41461 cctgtggttg tatcgatggg cggcatggcg gcatctggtg gttactggat ttccacacca
    41521 gctaattaca ttgtggctaa ccccagcacc ctgaccggtt ctatcggtat cttcggcgtg
    41581 atcaccaccg tagaaaatag tctggattcg attggtgttc atactgatgg tgtctcaact
    41641 tcaccgctgg cggatgtttc tatcaccagg gcactgccgc cggaagcgca gcagatgatg
    41701 cagttaagca ttgagaatgg ctataaacgc tttatcacgc tggttgctga tgcgcgtcat
    41761 tcaacgccag agcagattga taaaatcgcc cagggccatg tctggaccgg tcaggatgca
    41821 aaagctaacg ggctggtcga tagtctcggg gatttcgatg atgcggtcgc caaagcagca
    41881 gagctggcaa aagtgaaaca gtggcatctg gaatactacg ttgatgaacc gaccttcttc
    41941 gacaaagtga tggacaacat gtctggttct gtccgggcaa tgttgccaga tgcgttccag
    42001 gccatgttac ctgcaccgct ggcctcggta gcctctaccg ttaaaagtga aagcgacaag
    42061 ctggcagcgt ttaacgaccc acaaaaccgt tatgcgtttt gcctgacctg cgccaacgtg
    42121 cgttaagtct tgtactgagt ggccgacaga tcgtcggcca cattttttta cctcgacgaa
    42181 tcctcttccc gctgtctcgc cccatatttc cttatccgca tagtatcagg tgcgctcccc
    42241 cttcctcacg tatatacttt tgctctttcg atatcattca tatcaatatc atgcaaaaga
    42301 aatcaattta cgtcgcctac acgggcggga ccatcgggat gcagcgttcc gagcagggtt
    42361 atattccggt gtcaggtcat ctacagcgcc aactggcgct gatgccggaa ttccatcgcc
    42421 cggagatgcc agatttcact attcatgaat atacgccatt gatggattct tctgatatga
    42481 cgccagaaga ctggcagcat attgctgaag atattaaagc gcactatgac gactatgatg
    42541 gttttgtcat tctgcacggc acagacacga tggcgtatac cgcctctgca ctgtcgttca
    42601 tgctcgagaa tctcggtaaa ccggtcattg tgacagggtc acaaatcccg ctggctgagt
    42661 tacgctctga cggacaaatt aatctgctga atgcgttgta cgttgcggcg aattatccta
    42721 tcaacgaagt aactctcttt tttaataatc gactgtatcg tggcaacaga accaccaaag
    42781 cccatgccga tggttttgat gcgtttgcct ctccaaacct tcctccgtta ctggaagcag
    42841 gtatccatat tcgccgtttg aatacgccac ccgccccgca cggtgaaggg gaattgatcg
    42901 ttcatccaat cacgccgcaa ccaattggcg tagtgacgat ttatccgggg atttctgctg
    42961 acgtcgtgcg caattttctg cgccaacctg tgaaagcatt gattctgcgc tcatatggcg
    43021 tgggtaatgc gccacaaaac aaagccttcc tgcaggaatt acaagaagcc agcgatcgcg
    43081 gtattgtggt ggtcaacctg acacaatgta tgtccggtaa agtgaacatg ggtggttatg
    43141 ccaccggtaa cgccctcgcc catgccggcg ttattggcgg tgcagatatg actgtagaag
    43201 ccacgctaac caaactgcat tacctgctta gccaggaact tgatactgaa accattcgca
    43261 aggccatgag ccaaaacctg cgtggcgaac tgacgccgga tgattaagga gactgcaatg
    43321 caccctcgcg ccctgttact ggtcgattta caaaatgatt tctgtgctgg tggcgcgctc
    43381 gccgtgccgg aaggtgacag tacggtggat gtcgctaacc gcctgattga ctggtgccag
    43441 tcgcgcggtg aagcggttat cgccagtcag gactggcacc cggcgaatca cggcagtttt
    43501 gccagtcagc acggtgtaga gccttatacg ccaggccaac tcgacggttt gccacaaacc
    43561 ttctggccag atcactgtgt gcagaacagt gaaggcgcac aattacatcc gttactgaac
    43621 caaaaagcga tcgcagcggt gttccataaa ggcgaaaatc ctttagttga cagttacagt
    43681 gccttttttg ataacggccg tcggcagaaa accacgctcg atgactggtt acgcgatcat
    43741 gaaatcgatg aattgatcat tatgggtctg gctactgact attgcgtgaa gtttaccgtg
    43801 ctggacgcgt tacagttagg ttataaggtt aatgtgatta ccgatggttg tcgtggcgtg
    43861 aatatccagc cacaggacag tgcgcacgcg tttatggaga tgtcagcagc tggggcaacg
    43921 ctatatacgc tggcagattg ggaagagacg caggagtaat attagttgga ttcatctctg
    43981 gcctacattt cctcaatgac attgtaggcc aggtaatact tgttagtgtt gcatttaacc
    44041 tactcgcagc taaaacacga catccgaaca agtctatttg ccctccctta ataacaggct
    44101 taaatactag acaactcatt cacttaaaca tggcaatagg ctttgctgag ataccaaaat
    44161 cttcttttaa ctgttgtttg ctcttcatga ccatctgacc atttgtgtca attgtcatat
    44221 gctgtgcttc ggtattattg cgggcttgcc acagcatcac cagttgcagg cagttctctt
    44281 tttgttcctc ggtcaacgta acgccatcag gccatttccc cagttcaacg gcggtcgaca
    44341 aacgctggta tacctcaggc gtcatgctgt tgataatgtc atcaagattc ataatttttc
    44401 cgctcctgtg gaataagttg ctgaatcgtt ttttcaaccg ttgatttctt cgccgttttc
    44461 gccatcagtg aagcgtaaag aagcagagtt aacacaataa cgttcgcccg ttggctgcgg
    44521 cccgtcaggg aagacatggc ccagatgggc atcgcagtta ccgcaacgaa tttctatgcg
    44581 ctgcattcca tgtgacaagt ctttgatata acgaatagat tcttcactca ccggttcgta
    44641 gaaactgggc cagccacagc cggaatcata cttggtttgg gaatgaaaca gcggggcatc
    44701 gcagatcaaa cagtgatata cgccgtcacg cttgttatgc agtaaacgac ccgtaaatgg
    44761 cggttctgtc ccatgattct gcgtcacgta gaactgcatc tcggacaaat tttttttcag
    44821 ttcttctgcc gaaggtttat tagccatttg ctcacatctc actttaatcg tgctcacatt
    44881 acgtgactga ttctaacaaa acattaacac caactggcaa aattttgtcc taaacttgat
    44941 ctcgacgaaa tggctgcacc taaatcgtga tgaaaatcac atttttatcg taattgccct
    45001 ttaaaattcg gggcgccgac cccatgtggt ctcaagccca aaggaagagt gaggcgagtc
    45061 agtcgcgtaa tgcttaggca caggattgat ttgtcgcaat gattgacacg attccgcttg
    45121 acgctgcgta aggtttttgt aattttacag gcaacctttt attcactaac aaatagctgg
    45181 tggaatatat gactatcaaa gtaggtatca acggttttgg ccgtatcggt cgcattgttt
    45241 tccgtgctgc tcagaaacgt tctgacatcg agatcgttgc aatcaacgac ctgttagacg
    45301 ctgattacat ggcatacatg ctgaaatatg actccactca cggccgtttc gacggtaccg
    45361 ttgaagtgaa agacggtcat ctgatcgtta acggtaaaaa aatccgtgtt accgctgaac
    45421 gtgatccggc taacctgaaa tgggacgaag ttggtgttga cgttgtcgct gaagcaactg
    45481 gtctgttcct gactgacgaa actgctcgta aacacatcac cgctggcgcg aagaaagtgg
    45541 ttatgactgg tccgtctaaa gacaacactc cgatgttcgt taaaggcgct aacttcgaca
    45601 aatatgctgg ccaggacatc gtttccaacg cttcctgcac caccaactgc ctggctccgc
    45661 tggctaaagt tatcaacgat aacttcggca tcatcgaagg tctgatgacc accgttcacg
    45721 ctactaccgc tactcagaaa accgttgatg gcccgtctca caaagactgg cgcggcggcc
    45781 gcggtgcttc ccagaacatc atcccgtcct ctaccggtgc tgctaaagct gtaggtaaag
    45841 tactgccaga actgaatggc aaactgactg gtatggcgtt ccgcgttccg accccgaacg
    45901 tatccgtagt tgacctgacc gttcgtctgg aaaaagctgc aacttacgag cagatcaaag
    45961 ctgccgttaa agctgctgct gaaggcgaaa tgaaaggcgt tctgggctac accgaagatg
    46021 acgtagtatc taccgatttc aacggcgaag tttgcacttc cgtgttcgat gctaaagctg
    46081 gtatcgctct gaacgacaac ttcgtgaaac tggtatcctg gtacgacaac gaaaccggtt
    46141 actccaacaa agttctggac ctgatcgctc acatctccaa ataagttgag atgacactgt
    46201 gatctaaaaa gagcgacttc ggtcgctctt ttttttacct gataaaatga agttaaagga
    46261 ctgcgtcatg attaagaaaa tttttgccct tccggtcatc gaacaaatct cccctgtcct
    46321 ctcccgtcgt aaactggatg aactggacct cattgtggtc gatcatcccc aggtaaaagc
    46381 ctcttttgca ttacagggcg cacaccttct ctcatggaaa cctgcgggtg aagaagaagt
    46441 tctgtggttg agcaataaca caccgttcaa aaatggcgtc gctattcgcg gtggcgtacc
    46501 ggtttgctgg ccgtggtttg gtccggctgc acaacaaggt ccggctgcac aacaaggtct
    46561 gcctgcgcac ggttttgccc gcaacctgcc gtggacgctg aaatcacatc gtgaagatgc
    46621 tgatggcgta gcgctgactt ttgaattgac acaaagcgaa gagacgaaaa aattctggcc
    46681 gcacgacttt acgctattag cgcatttccg cgtgggtaaa gcctgtgaaa tcgatcttga
    46741 agcacacggc gaatttgaaa ccacctctgc cctgcatacc tactttaacg tgggtgatat
    46801 cactaaggta agcgtcagtg gactgggcga tcgcttcatt gataaagtga atgacgcgaa
    46861 agaagatgta ctgaccgacg gtattcagac cttccctgac cgtaccgatc gcgtgtatct
    46921 gaatccgcaa gattgcagcg tgattaatga cgaagcgctg aatcgtatta tcgccgtagg
    46981 ccaccagcat catctgaacg tcgttggctg gaacccagga ccggcgcttt cagttagcat
    47041 gggcgatatg ccggacgatg gctacaaaac ctttgtttgt gtagaaacgg cttacgcttc
    47101 agaaacgcaa aaagtgacca aagaaaaacc tgcacatctg gcgcaatcca ttcgcgttgc
    47161 gaaacgttaa tttgcgttaa tgttgtgtgc cgggtgcgtt gcatccggca cgtatcaaca
    47221 tcacaccata tccagcgcag tttttccttt tggtgccgga tatgccttat ccagcatagc
    47281 taattccgct gacgaaagtt caacctcaag cacagccgca ttttgttgga catgggcaat
    47341 cgtggccgct tttggaatcg ccatcacacc ctgatgactg atcacccacg ccaacaatac
    47401 ttgtgccgcg ctgatattgt gagcatgtgc aatttcgttg actactgcgt tttttagcag
    47461 cccattgcgc aaccgcccgg cctgagctaa cggactgtaa gccatcaccg gtatctgctg
    47521 ttgctggcac caggggagta gatcgtactc aatccctcgt gaaccgagat ggtaaagcac
    47581 ctgattagtg gcacactgat ttccccccgg cagctgccag agttcctgca tgtcggcata
    47641 atcaaggtta gaaacgcccc agcggcggat ttttccctgg gcgatcaatt tttccatcgc
    47701 tgcgacagtc tcttcaaaag cgaaactgcc agaccagtgt aataagtaga gatcgagata
    47761 atcagtattg agacggcgta aactggcttc gcatgcattt atcgcttttt gcccgccagc
    47821 attccacgga tagactttag agacgagaaa gacgttatca cgcagaccgg ttaatgcttc
    47881 cccaaccacc ttttcagcac cgccttcggc atacatttcg gcggtatcaa tgagggttaa
    47941 accgagttca atgcccgcgc gtagtgcagc aacttctgtt ttgcgctgac tggcatcttc
    48001 gcccatatac catgttccct gccctatggc tggcagtgag acatcgccac taaattgaat
    48061 cattttttgt tgcattgttt cctcccaggt attgcaccac cgtaatgcaa aacagggcat
    48121 gacgccctgt ttttatgcac aaaatgccct ggaaagatgc attatcagaa tttgtaggtg
    48181 atcccggtag aaatcaggcc agtccaggat ttatccacca ttgggctgtc agtaacttca
    48241 tcagacagac gggtgtagcg cgcggtaccg taaacactcc agtcgccgag gaagttgtag
    48301 ctggcgctca gctccaggta agggctccag ctgtcgttcg ggttatagcc acgcagaccg
    48361 ctgcgagcgg actctttgcg cgatacgcca taatagtatt cgttctggtt ttcgctgttc
    48421 cactgcacac caatacccgg agtcacggtc aggccaccgt tggtgtaacg atacaaccag
    48481 gccatatccc agacgatgcc gttgctgtta tccagggtat cgccagccag ggtggtacgc
    48541 aggtaaccgt actgggtaaa gtgagcataa gacagaccag ccatcatggt gctcttacgg
    48601 tcatccaggt gacgcatttg gtgatcgcca ctgtctttag ctttgaagta aagcggcgac
    48661 cagtaagcgg taattgaaag tttgtccgtt gcgtcattcc acaggtagta accaccacct
    48721 aagccacgga accagaagtt atcgccgtca tagttgatta ccggtactgg gtaaacatcg
    48781 gtatcgtaat ctttatatgg gtgttcaacg acacctacgc ctgcgcccag ggaaaattta
    48841 ccttcagcgt gcgctacgcc tgcagacgtt gcgataagca ctccaagtgc cagaagtttg
    48901 agtttggtca caattaatca ttccttaaac aaatgtttag cgggcgacaa agtttacccg
    48961 tcaatacatc gaaacccaac ctttttacgt tttcattttt tatagtaact gtttaatttt
    49021 cctgacgcag atgacaccac tcttacagcc aaatgaattt agcgttaccg gcgaccctgg
    49081 tatttacatt aattatgcaa aatttatgga tgagttgttg atatgccatt gaaattaaga
    49141 aagccgtgca ggcaagtttt ccatttgcca tctacgctta attttgaagg tgtatcaccg
    49201 ggcacgttgt tctcatcgtc gataaaatgg catgagagtt gctgtgtttt agcaagagac
    49261 gtcgttcagt ttacttcttc cgggagcctc tactattcat atgaacggct cttaacctgt
    49321 gctaaaaaac gaaaggacgg cataccatga atatattcga tcactatcgc cagcgatatg
    49381 aagctgccaa ggacgaagag ttcacactgc aggagtttct taccacttgt cggcaagatc
    49441 gcagtgctta tgccaacgcg gctgagcggc tattgatggc tatcggtgag cctgtcatgg
    49501 tcgatacagc ccaggaaccc agactttctc gactcttttc taaccgggtc atagcacgtt
    49561 atccggcatt tgaagagttt tacggcatgg aagacgcgat tgaacagatt gtctcttatc
    49621 tgaaacacgc ggctcagggg ctggaagaga agaaacaaat cctgtatctg cttgggcctg
    49681 tgggtggggg taaatcatcg cttgctgagc gactgaaatc attaatgcag ctcgtaccga
    49741 tttatgtatt gagcgccaac ggtgagcgta gcccggtcaa cgatcatccg ttctgtcttt
    49801 tcaatccgca ggaagatgcg cagattctgg aaaaagagta tggcattcct cgccgttatc
    49861 tcggcaccat catgtcgccg tgggcggcaa aacgcctgca tgaatttggt ggcgatatca
    49921 ctaagttccg ggtagtgaag gtctggccgt caattctgca acaaattgct atcgccaaaa
    49981 cggaacccgg tgatgagaac aaccaggaca tctccgcgct ggttgggaaa gtcgatattc
    50041 gtaaactcga acactacgcg cagaatgacc cggacgccta cggctattcc ggtgcactgt
    50101 gccgcgccaa ccaggggatc atggaattcg ttgagatgtt taaagcaccg attaaagtgc
    50161 tgcatccctt gctaaccgcc actcaggaag gtaactacaa cgggacggaa ggtatctccg
    50221 ccctgccgtt caacgggatt attctcgccc actcgaacga atccgaatgg gtcactttcc
    50281 gtaataacaa aaacaacgaa gccttcctcg atcgtgttta catcgtgaag gtgccgtatt
    50341 gcttgcgcat ttccgaagag atcaaaatct acgagaaatt gcttaatcac agtgaattga
    50401 ctcacgcccc ctgcgcccct ggcacgctgg aaacgctgtc acgtttttcc attctttcgc
    50461 gccttaaaga gccagaaaac tccagcattt attcaaagat gcgggtttat gatggcgaaa
    50521 gtctgaaaga cacagatccc aaagccaagt cgtatcagga atatcgtgac tacgccggtg
    50581 tcgatgaagg gatgaacggt ctgtcgacgc gttttgcgtt taagatcctc tcccgcgtgt
    50641 tcaacttcga tcatgtagaa gtggcggcaa acccggttca tctgttctac gtcctggaac
    50701 agcagatcga gcgcgagcag ttcccgcaag aacaggcaga acgctatctg gagttcctga
    50761 aaggttatct gatcccgaaa tatgccgagt ttatcggcaa agagatccag acggcctacc
    50821 ttgaatccta ttccgaatat gggcaaaaca ttttcgaccg ttatgttacc tacgcggatt
    50881 tctggattca ggatcaggag tatcgcgatc ctgataccgg gcagttgttt gaccgcgagt
    50941 ctcttaacgc cgagctggag aaaatcgaga aaccggcggg gatcagtaat ccaaaagatt
    51001 tccgcaacga gattgttaac ttcgtactgc gcgccagagc gaataacagc ggacgcaatc
    51061 cgaactggac cagctatgaa aaactgcgca cggttattga gaagaaaatg ttctccaata
    51121 ccgaggagct gttgccggtt atttcgttta acgccaaaac gtcaaccgac gagcaaaaga
    51181 aacatgacga ctttgtcgac cgtatgatgg aaaaaggcta cacccgtaaa caggtgcgtt
    51241 tactgtgcga atggtatttg cgcgtacgta aatcgtctta acaaccctgg cccggtacac
    51301 atgctaccgg gcctacaacg acagcgaacc gtgggccaga gaagcggcaa cacaggcgta
    51361 gcataaagtt ggcaaatgta gtacgggggg catatgacct ggtttattga ccggcgtctg
    51421 aacggcaaaa acaaaagcat ggtgaatcgc cagcgttttt tacgccgtta taaagcgcaa
    51481 attaaacagt cgatctccga ggccattaat aagcgttcgg tgactgacgt cgacagcggc
    51541 gagtccgtat ccattcccac ggaagatatt agcgaaccga tgtttcatca ggggcgtggc
    51601 ggtctgcgcc accgcgtgca tccgggcaat gaccatttcg tccagaacga ccgaattgaa
    51661 cgtccccagg gtggcggcgg aggttccggc agcggtcagg gccaggccag ccaggatggt
    51721 gaaggtcagg atgaattcgt ctttcagatt tcgaaagatg agtatcttga tctgctcttt
    51781 gaagatttgg ctttaccgaa tctgaaacaa aaccaacaac gccagctgac cgaatataaa
    51841 acgcatcggg cgggttatac cgctaacggc gttccggcca atatcagcgt tgtgcgttca
    51901 ttgcagaact cactggcgcg gcgcacagcc atgacggcgg gcaagcggcg ggaacttcat
    51961 gcgctggaag agaatttggc catcatcagc aacagtgaac ctgcgcaact gctggaagag
    52021 gaacgtctgc gcaaagaaat tgcagaatta cgtgccaaaa ttgaacgcgt ccctttcatt
    52081 gacaccttcg atttacgtta caagaactac gaaaaacggc ccgatccctc cagccaggca
    52141 gtgatgtttt gcctgatgga cgtttccggt tcaatggatc agtccactaa agatatggct
    52201 aaacgttttt atattctgct gtatctgttc ctcagcagaa catataagaa cgtggaagtc
    52261 gtatacatcc gccatcatac ccaggcgaaa gaagtcgatg aacatgagtt tttctactcg
    52321 caggaaacag gcggcaccat tgtttccagc gccctgaaac tgatggatga ggtagtgaaa
    52381 gagcgttata acccggcaca gtggaatatt tacgcagccc aagcatcaga cggcgataac
    52441 tgggccgatg actctccgct ttgccatgaa attctggcga aaaaaatatt acccgttgtt
    52501 cgttattaca gctatatcga aattacacgt cgcgcgcatc agacactgtg gcgagaatat
    52561 gagcatctgc aatctacttt cgacaacttt gcgatgcagc atatccgcga ccaggatgat
    52621 atttatccgg tgttccgtga actgtttcat aaacaaaatg caacagctaa agactaaaac
    52681 tgtcagccgg gtcattatcg cctggctgat ttttagttca ctgtaaatta tctcatttat
    52741 tacatacatt agcttacaat cgctttaaat atgacagcat aacctttaca caatttagtt
    52801 ccagaaaaca atcattcgga aaaatgattc gg
//
LOCUS       DAZGQL010000021        52422 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.021, whole genome shotgun sequence.
ACCESSION   DAZGQL010000021 DAZGQL010000000
VERSION     DAZGQL010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 52422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52422
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.021"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..1111
                     /locus_tag="ACHKIR_001475"
     CDS             <1..1111
                     /locus_tag="ACHKIR_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9989229.1"
                     /translation="FFDAVNLSSTLGIIFFFILLSISGFSLIIFISSFILILIYSGYE
                     NKLKVYSSITHRISTVCYQNSLFICCLLIIAYYIYYMTKWNGYIITAFSIISIFIHSY
                     VTCAIRLFRKQKSRLNNTVRYEKLSRKKGFNFWLSLQLIIPGVVQILPMMFLFNQLNF
                     SEGTSDWYEMSILIAITVVIVTIGILPGIIHINERQNGNKMTGIIVVLIFIPVATAAL
                     SVWYRPIPNMIANMTMNLSGISDQRTHEYYIERATHPAGMFNGKIWNTRYYKNIPDRF
                     FITGVNTFTLGNIKLICPTAIVKARIESLKFTVNDIDEYEQKGKKLKKTAMKCIPFDK
                     NDIHTWDSPLSEPIYYEKIKQTIDNSMLKILHVVK"
     gene            1179..1487
                     /locus_tag="ACHKIR_001476"
     CDS             1179..1487
                     /locus_tag="ACHKIR_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000966040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RAQPRD family integrative conjugative element
                     protein"
                     /protein_id="HFO9989230.1"
                     /translation="MQSQTALLLLIIPFPVLVIASPESTQLALALKQLDGVKTSLEHA
                     RTSASNDPQARYFFDYLQAQQDISVVEQGIRRYLAPARAQPRAIQPLSANYQQENPDD
                     "
     gene            1480..1713
                     /locus_tag="ACHKIR_001477"
     CDS             1480..1713
                     /locus_tag="ACHKIR_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009654440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03758 family integrating conjugative element
                     protein"
                     /protein_id="HFO9989231.1"
                     /translation="MTDSQLAAFQAASLSKPETVSLLVLGLFTSVLLLWCAWTLMVAW
                     QGVCRQRLSWQSFGAVAAHSLLLVLVSFWLVLS"
     gene            1723..2040
                     /locus_tag="ACHKIR_001478"
     CDS             1723..2040
                     /locus_tag="ACHKIR_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03745 family integrating conjugative element
                     membrane protein"
                     /protein_id="HFO9989232.1"
                     /translation="MRWFLLLLFATFNALADLPTMEDPSRGQGKGIMETLQNYGFDIV
                     VLLALAICAVGFLVVANNCIATYSEIQSGRKQWKDLGSMAGVGSILLVVIIWLLNQAS
                     DVL"
     gene            2042..2395
                     /locus_tag="ACHKIR_001479"
     CDS             2042..2395
                     /locus_tag="ACHKIR_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03750 family conjugal transfer protein"
                     /protein_id="HFO9989233.1"
                     /translation="MATIPFLPDRLNREPVVWRGLTTSELFLALALGLGGGCAFGILL
                     ALITHYWPLIPGSALSGAALLIQGGGRILARAKRGKPDTWLPQALLAWLERHHLRGAQ
                     LIQHSAVWVVRRSPR"
     gene            2392..3051
                     /locus_tag="ACHKIR_001480"
     CDS             2392..3051
                     /locus_tag="ACHKIR_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006686463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PFL_4703 family integrating conjugative element
                     protein"
                     /protein_id="HFO9989234.1"
                     /translation="MSRFRHALAERDNHILTLRAVIVLLCLCLAGVGYGWYSAPQKLT
                     LWFPPDLRSGSTRPWWEVPPASVYAFTFYIFQQLNRWPDNGEEDYSRNLRSLRAYLTP
                     ACYTRLEHDFQVRRNGGELRDRTRGVYELPGHGYSSLVHEGEKRPFPRVEALSRDSWR
                     VTLDVAVEEYYQNEPVKKALVRYPLQVVRYAIDAEKNPWGLAIDCTDAAPQRLVAEEK
                     P"
     gene            3048..3815
                     /locus_tag="ACHKIR_001481"
     CDS             3048..3815
                     /locus_tag="ACHKIR_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009654428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03749 family integrating conjugative element
                     protein"
                     /protein_id="HFO9989235.1"
                     /translation="MKPVLFTLLFSFPAFATEVVQWENIPLPVTLHVGQERIVDVGKP
                     VRIGYPATLEGKVRLQSAGGKVFLLANTAFPATRIQLRDTGNGELILLDIHATQGTST
                     LEPMQISYTPQTPATAKTSVPVTASTEPLPVLLVRYAAQNLYAPLRTVEALPGVTPAP
                     VRLAKRITTLLPQQPVTATPLASWQVNATTVTAIRLQNHSGQRITLDPRELQGQFTAA
                     AFQHDWLGPRGLAEDTTVAYLVTEGPVSRALLPEPKP"
     gene            3812..5107
                     /locus_tag="ACHKIR_001482"
     CDS             3812..5107
                     /locus_tag="ACHKIR_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009654426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03752 family integrating conjugative element
                     protein"
                     /protein_id="HFO9989236.1"
                     /translation="MMPRQSLLLKILLPLIVLVTVWLLLRPASKNTQETSLSRTVAAL
                     TPQELRDLGIDGDTPADTVATLVGQMKQYRRELQAIQTNNESQQAREKRLNAQQATLA
                     SQLREDMRRQQDQMRDELVKSQRGLLDKLDRLMAKRPDDIPPGLGLTPGELPADVQPD
                     IRWLEPLDAPPPVDEKLLRVPLKAADKPPPKPDVKVYTLPQNATLTGSVAMTALIGRV
                     PLNGTVSDPYPFKLLIGEDNLTANGVELPDVAGAVVSGIASGDWTLSCVRGQITSMTF
                     VFHDGIIRTVPQGGKSGNKQQEIGWLSDNQGLPCIPGERKSNAKEYLTSQFLLAGSGA
                     AAQAFANGESTTVVEDGSITSAVTGNNGQYVLGQAIGGGLKETADWMKQHYGQMFDAV
                     YVPPGHKVAVHLTEALSIDYEPEGRQVKYQQASTPGGLE"
     gene            5104..5463
                     /locus_tag="ACHKIR_001483"
     CDS             5104..5463
                     /locus_tag="ACHKIR_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012737281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03751 family conjugal transfer lipoprotein"
                     /protein_id="HFO9989237.1"
                     /translation="MKFTILILLLLLAGCSTSQEELMPVDPNASMLTLWQQQSGGNAA
                     LLDARAQLRRPPDGPVAQPEADTIRSQFPRLPNPDMVLYVFQHLNSNTPVPGYSTVFP
                     FYTRPQYALPGERLTPP"
     gene            5460..8102
                     /locus_tag="ACHKIR_001484"
     CDS             5460..8102
                     /locus_tag="ACHKIR_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003830179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer ATPase"
                     /protein_id="HFO9989238.1"
                     /translation="MNERRGLTEQALNTSYRKGPSFVDWLPWVEFLPESQTLLLEDGR
                     SVGAVFELYPVGTEGRSTAFLEQVRDTVTDALQDAFDEHERAPWVVQFFCQDEDDSEA
                     WLASLASYAAPEIRDSAFTREWLTQMANHMVNLTRDEGLFLDNTVTRTRWRATWRRTR
                     MVVYRWLPGLSPDEPSPEETLNNICERLTAGLKAAGISAIRQNEQDIHRWLVRWFNPA
                     PPAEDKKAFWQQLDLPETEPLPVLADFAERLLASEPRSDAANGVWWLDNRPHKVVVVD
                     RLRKAPPPGLLTGETARGDGINALVDLLPGGTIMNLTVVVYPQDKLEQHISRLADRAI
                     GDNTDSEYTRRDCSEVRSFLKDGHKLYRSTLAFYLSAPDTDILFRQQRQLQSLLLNAG
                     LVPVQEQHEVAPLSHWLRWLPMCFDPLRDPRGLYTRLNFVQHIAGLLPLYGRETGTGH
                     PGISYFNRGGTPLCFDPLNKLDRAQNGHLLLLGPTGAGKSATLNAKIAQLMALHRPRL
                     FIIEAGNSFGLMADYAAAHGLTVNKISLRPGSGVTLPLFADAWRLVEQAVTDAEPPED
                     EESDGDDQRDVLGEMEITARLMITGGEAKEDARLTRPDRAMIRQAIIAAAHTCYEEKR
                     QTLTQDIRDALFALSRDNSLPEARRQRAYEMAESINLFCAGFEGELFNREGQMWPEAD
                     ITLVDLATFAREGYEAQLAISYISLINHINNLGERAQHLARPIVNITDEAHIITVNPL
                     LARFLTKGSKMWRKLGIWLWLATQNLADFPDEAKKLLNMIEWWELLVMPPEEIEQVCR
                     FKALTEEQSQLLRSAVKSPGQYTEGVVLSPRIEALFRVVPPALYLALAMTEKHEKADR
                     MRIMKETGCSEVEAAITLSSVISQ"
     gene            8151..9071
                     /locus_tag="ACHKIR_001485"
     CDS             8151..9071
                     /locus_tag="ACHKIR_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9989239.1"
                     /translation="MTAIACWFNREESDSIWAVSDSRITQQNSRLTDHCPKLFSIPVS
                     VIRSSDVYRIHPQKIFEFGFGFAGSTMIGINVKEMLAVSLARLHEIANNNPEQQISTE
                     AFPSLNEIASLAKNIAEQYMSDIGQSFPQAVRIQMIIFGFCWRTQTYKIIKLNNSIEH
                     PANLQIEDIQNLSTGRPVLLGDNLHELNEFIENTRQQFTPNTINWWRTPFIALNNWIN
                     QETVNTIGGYIQMVTAHPLGTRISFLTNLNENIIRTSYAGINTTESFGPTIGGFILMP
                     MDGMSLPDENGWDVGNQVTRTAADRVATNR"
     gene            complement(9074..9298)
                     /locus_tag="ACHKIR_001486"
     CDS             complement(9074..9298)
                     /locus_tag="ACHKIR_001486"
                     /inference="COORDINATES: protein motif:HMM:NF024835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO9989240.1"
                     /translation="MKNTSSIQAQFGAHLKKLRLQSGLSQEAFADKCGLDRTYVSGIE
                     RGVRNPTLEVISVLGKGLDMDIKELFNFER"
     gene            complement(9338..9601)
                     /locus_tag="ACHKIR_001487"
     CDS             complement(9338..9601)
                     /locus_tag="ACHKIR_001487"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO9989241.1"
                     /translation="MEKTGSIKKVLGKRIRALRREMDLSQEALADKCGIDRTYMSGIE
                     RGVRNPTLVVICAISDGLGMDLSHLFMFDDGAEKDAESTKCDS"
     gene            9709..9984
                     /locus_tag="ACHKIR_001488"
     CDS             9709..9984
                     /locus_tag="ACHKIR_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO9989242.1"
                     /translation="MTKKDWHPADIIAALHKRGTSLAKLSREAGLASSTLANALNRPW
                     PKGEWLIAIALGKHPSEIWPERYLNTGNKREPRKPLHLMTTTENSGT"
     gene            10203..10577
                     /locus_tag="ACHKIR_001489"
     CDS             10203..10577
                     /locus_tag="ACHKIR_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFO9989243.1"
                     /translation="MMTYCARIVAIGLFVADGLADRTLITFDSNGPKDCLDYSLSLEP
                     SFREDDLLILPGDRLLLASHDYLVTAVGKEAQQSLFELGHLTMVFNGSLNACHSGAVH
                     LSGPIPKLHDLYGELLIEEGRP"
     gene            10621..11979
                     /locus_tag="ACHKIR_001490"
     CDS             10621..11979
                     /locus_tag="ACHKIR_001490"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO9989244.1"
                     /translation="MELNRSLKARHLTMISVGGVIGAGFFLGAGAAIARTGPSVVLAY
                     LIGGVITLFIMLLLAEMAVARPVSGSFESYARTAFGPWCGFITGWTYWLAFLIGPASE
                     AIAAGTFLNLWFPGVPVWLFCLVIASSLTAINMVGVHFFGEVEFWLSLVKVVALLAFI
                     VAGCYSLGLDNASPASISHPTDSGSFFAGGLPGFLGAMLMVIFSFGGTEAIGTAAGES
                     ENPQRDIPRTLRGTVVRILLLYVGSLTVLLLVLPWQQAGVSSSPYVEASGILGGHMAR
                     HIMNFVVLTAALSCIDTGVYATSRMLHAMASDGYFPAWFARLHPAHKTPDNAILASSL
                     VLFTGVIIAILSPDAYVILAGISGFGFLFTWLMIALSQPTLHRIALHDGTLKYKAPAA
                     RYIQPLTVGLIALIMAGQFFTDGGGITLVTVLIWLALASAYYIFVVRKNRRLSDEKSH
                     AQ"
     gene            11957..12748
                     /locus_tag="ACHKIR_001491"
     CDS             11957..12748
                     /locus_tag="ACHKIR_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004241872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HFO9989245.1"
                     /translation="MKKVMLNELTGREIRELLQSGKELTAIVNFGSCENHADHLPLGP
                     DMFVPEIVACRVAQRVDNTLVLPCTPFGTSVHYNAYPLAVTLRYETNIALAEDIFISL
                     LHHGIRRILVINGHDGNIPAIEIAARNVKARHTQAAIVVVPAWWDLVGHHLGKGFFDS
                     WDNTGLGHGGEGETSVMMALIPELVELKYAQKQIPVEVFADYGVNRIWDIGEISATGA
                     TGDPRCATAEKGQRILACLVDVLCALLRKLDAANWQYALNKDANM"
     gene            complement(12851..13270)
                     /locus_tag="ACHKIR_001492"
     CDS             complement(12851..13270)
                     /locus_tag="ACHKIR_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001195655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9989246.1"
                     /translation="MANQQAAKWAGVAAIAACLTFVVTTIGLLLAWRSLHQWKPQYKE
                     NSRLLLIEALIAFQKCLITIPKNLDNDPTYQSRKEFLKASTEVELRGQIYLKQHSNEK
                     LKDELANLRSKCAEFVGGKVTKPELSFISAIILLIEV"
     gene            complement(13509..14399)
                     /locus_tag="ACHKIR_001493"
     CDS             complement(13509..14399)
                     /locus_tag="ACHKIR_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006662654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning protein RepB C-terminal
                     domain-containing protein"
                     /protein_id="HFO9989247.1"
                     /translation="MISQCFDSRFYTFKVESLVYARTLPVNVKFSTKYKQIKASVDEI
                     GLIEPIVVYINDANDKVILDGHLRVEVLKELGITTAHCLISPVEDTYSYNKRVSRLTI
                     VQAQNMLLKAVESGVSVEKLCAVLGVTPDTLIGKLKISDGIAPEVMALLAEKNVPQTI
                     FSVLKKLKFYKQIEMVNTMISINNFTRKFAMSMLHAVAPEHLVAPKKDASRETDIRKN
                     LERLEKEMASVQVETQHLQDEYAENNLRLVIIKNHIERLLANGQVLNWLYDNHQEYLS
                     VLKQISGLDDLNKLGQTIEE"
     gene            complement(14401..15288)
                     /locus_tag="ACHKIR_001494"
     CDS             complement(14401..15288)
                     /locus_tag="ACHKIR_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006662653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFO9989248.1"
                     /translation="MKSFDEQIELVDISDIYIANPRKRNKFIHNEIKENIKTIGLKRP
                     VSVRPVNNENYKYALICGQGRLEAYIEYGETKIPAIIKQVDEETAHLMSLAENIARRN
                     PRAAELMEDIRRMKTSGLTDREIGLRLGYNSSWVNGVITLLSSGEKHLLRAFETGDIP
                     LYLAVEISRSGDEETQELLTVALKEGHIKGGQINKIRRILEQRKKGDKGTISTAFFPG
                     KKQKKLTPEELANVYQENINEQKAILLKSTMVKEKILAVKQIFGYLLKDNDFVTLLHS
                     NNIHDIPSLLDGSDVSGEK"
     gene            complement(15288..16823)
                     /locus_tag="ACHKIR_001495"
     CDS             complement(15288..16823)
                     /locus_tag="ACHKIR_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006662652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO9989249.1"
                     /translation="MSDIVAGNRIAAQYLRMSTEHQKYSPENQREYIEKYAKEHCITI
                     KYTYDDAGRSGLSLDNRPGLRSLLYDVENGIIKVSLILVYDVSRFGRFQDIEEAAHYS
                     WLLKRNGVRIIYCAEPFSEEQTELFMLGLSYSRYGAATYSKNLSQKVFLGQANLVRRG
                     YYQGGMPGYGIRRLLIDEHTNPKGMLLPGQRKSLQTDRVILIPGPAEEVVIVNKIYDM
                     FIDEGKPEFVIASELNRKNIPAENGLQWSRCKIHQILTNEKYIGNNVYNRTSGRLKTR
                     SVRNPQDQWIRCDGAWDPVVSVEKFEAAKRIIEARAVQYSDDELLDKLRHLLKLRGRL
                     SGIVIDEEELFPSSSIFRSRFGGLVRAYTLIGYHPRHDYSYLEIKKQIAECHLTFMDN
                     LVQDIYRYGGWAEMHQDGSLYINDEFFVSANLVRCQRLSSGKLRWKIKFNVSHYCDVM
                     IFPRLDSTNNSVVDYFMFPAIDVLYNDLTLHENNGFILELYRFDDLSCLENLVKRTGL
                     ESK"
     gene            complement(16847..17794)
                     /locus_tag="ACHKIR_001496"
     CDS             complement(16847..17794)
                     /locus_tag="ACHKIR_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009654399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO9989250.1"
                     /translation="MRKILFFLLLMIAVLPEVQATCSFYGGVTSEVSGYLNFGNVVIQ
                     RDAQVGSTIATAITGAYNNGDGIAGCKREAWTARWELTQWGTLSGYGNGVYNTNLPGV
                     GLRLSAASTGKTLPYETSYAYNAGGTWVSIPGDGIKGELIKTGDITSGTLTDGVLARA
                     SVVNQFYFANVTLNGMNTVKSEACTVTTPSVDVPMGDHDKSEFSGAGSSTDWVSFDIG
                     LSCDVGARINVRIDATADASASSQGVMLIDSGGASGVGIQLHYRPDDAAVQYGQERFY
                     WQSVYGDEIVQLKARYYQTAGSITPGAANGTATFTMSYK"
     gene            complement(17813..18817)
                     /locus_tag="ACHKIR_001497"
     CDS             complement(17813..18817)
                     /locus_tag="ACHKIR_001497"
                     /inference="COORDINATES: protein motif:HMM:NF012635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO9989251.1"
                     /translation="MRVWRGMILLAGILFCQASWAFCTSSVSFSISPGTVVVQRDAVV
                     GEPISDWLVSNQGMAYQDCNYDATPQYVINAGIKNTGSRASVTWNGEAVFNSNVAGVG
                     FVFEGRSSVDSNPLPENWIGIASGQSQVFTLQISHSVGYHPSINDQARIRLIKTGDIT
                     PGTLSGNLGRFIAGTRENGQWSTEIPITFSGGQITTIACSVTTPDVNVPLGQHQKAEF
                     SGPGTGTGWQAFNIGLDCDKSARINVQIDAVQDPSNVAGVMKLDSAPGDMAATGIGVQ
                     LFFAPDNSAVQFGQSRYYYTSPNGGTETVQLKARYYQTAATITAGVANATATFTLTYK
                     "
     gene            complement(18814..21330)
                     /locus_tag="ACHKIR_001498"
     CDS             complement(18814..21330)
                     /locus_tag="ACHKIR_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014908064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO9989252.1"
                     /translation="MNNPTPRLMIILLTLLLAIVLLSGYLRNAEAREVFNPHALEIDN
                     PGQPVADLSTFAESGGQLPGTYRVTVFVNGEKQSDAQDIAFIDGAKKKLSPQITPAML
                     KAWGVKTDVFPALAALPPDQPLDDIGRYIPMANTELRFSKLQLNVSIPQAAMNASARG
                     YVDPSEWDEGVPAALLNYNLSGSNTWRDGQNGSDDNYYANLQSGINLGAWRLRNYSTW
                     NYDEDNGSHWDSVNTYLQRDIQRLKGQLTLGDSYTPSDIFDSVQFRGAQLASDDNMLP
                     DSLRGFAPVIRGIAQSNAQVTIKQNGYIIYQSYVAPGAFTITDLYPTSGSGDLEVTIK
                     EADGSERTFVQPFSAVPIMQREGRLKYALTAGKYRSGNEDSDEPQFGQVTAIYGLPHA
                     ITVYGGTLYSDDYQSGAAGLGFGLGELGSLSADITAAHTTLNNDETHNGQSYRVQYSK
                     DFQTTDTSFTLAAYRYSTAGFYTFQEANDLRADDDNGWQMTYNKRQRLQLDLSQSIGS
                     YGSFFISGYQQDYWQEDGYERTLSAGWNGNIDGISYSVTWSYSDYPESTQPADQQLAF
                     NIQVPLSRFMPNAWASYSVNTAKHGDTRQQVGLNGTALADNNLSYSLQQSYTNHGVGG
                     SGNINADYKGGQGEITGGYNYDDDTQQVNYGLKGGIVAHPHGITLSQPLGQSLAIVKA
                     PGADDTKVQNNTGVYTDWRGYAVVPYVNPYKKNRIALDTSTLGDEVDTDTAVQTVTPT
                     QGAVVMADFNTRVGRRVLMTLLYRGLPVPFGAEAKLKEGGSGIVGDDGQVYLTGVPEE
                     GDIAVNWNGAQQCVVHYRLPEQENQPSVILVNQECREVAK"
     gene            complement(21342..22022)
                     /locus_tag="ACHKIR_001499"
     CDS             complement(21342..22022)
                     /locus_tag="ACHKIR_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO9989253.1"
                     /translation="MNRYADVVLALLLAFLSVQAQAGIVIGGTRVIYDGDKKETAASV
                     RNPEKSGVYLVQSWVDSGEQGGKTPFIVTPPLFRINPGEENMLRIVRTGGNLPQDRES
                     VFWLNVKSIPATDDSHSRTNVLQVVVKSRLKLFYRPAGLEGQPESAYHQLSVAHSGNH
                     LTVSNPTPYYVTLFTLKVDGQEIKEADMVPPKGSVSFTLPSATASAVTWQAISDYGGV
                     SQTESRKL"
     gene            complement(22136..22648)
                     /locus_tag="ACHKIR_001500"
     CDS             complement(22136..22648)
                     /locus_tag="ACHKIR_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO9989254.1"
                     /translation="MKKTMLSACFLLVAGNAVANDGTIHFTGSITDQTCTVDSGSQNL
                     NVDLGNVGQTALNGAAGMKASPTRFTLNLSGCPDTVTGANVKFDGTSDGVNQNLLALD
                     SGTGIATGVGIEIADKNGAVIPLHTASMDYPLAAGSNTLDFVARYVSTGTAVTTGTAN
                     GTSQFTINYK"
     gene            complement(23016..23606)
                     /locus_tag="ACHKIR_001501"
     CDS             complement(23016..23606)
                     /locus_tag="ACHKIR_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012737266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type DNA invertase"
                     /protein_id="HFO9989255.1"
                     /translation="MPEKRKHLTRHEVGQILLAADRGIHFERDYCLIQMCFLHGLRVS
                     ELCGMRLSDIDLIGRSVYVRRLKNSLSTQHPLFDNELPALMRWLAVRNRWRDADSDWL
                     FLSQKGGALSRHQVRLLLKRYGELAGVSISAYPHMLRHGCGYALADLGRDTRLIQDYL
                     GHRNIRHTVIYTATNTQRFMNVWGNVRENHTISARM"
     gene            23819..24742
                     /locus_tag="ACHKIR_001502"
     CDS             23819..24742
                     /locus_tag="ACHKIR_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014908065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03756 family integrating conjugative element
                     protein"
                     /protein_id="HFO9989256.1"
                     /translation="MRAQYILLLLCAAFPLRAITTPEIAASALTPDCVKYRVVGLCYW
                     LYCTPFGCTVRTSVKVSHFRPDLVVSAYSNTGQNPWAEMSLLSPPLPGVAEGGGDTNP
                     RSASQHSKVRFKNADAIGFPAGDELAKFFTQFGYICSPSSQPFQPYFLSQPDTLAWRS
                     GVPEMTYPEALTPGMREVGQNGDMWGNIYPRAGAINQTHDYKAAAVIAQRAADLVTRT
                     GQPHLYTPLTASSRAGYWPPSPVIEGDSDNHRWQMLTPKKSAACSVFPDGSATDTYAN
                     KLAEDGAYAWTLWRPYKCCPRRGQTFLGSTG"
     gene            24754..26115
                     /locus_tag="ACHKIR_001503"
     CDS             24754..26115
                     /locus_tag="ACHKIR_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006686442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrating conjugative element protein"
                     /protein_id="HFO9989257.1"
                     /translation="MRALFLVLLFFAGVCVAADDYSFRTRGAVDDLLYYQIGGGEAVM
                     PPPVRRSPSPLSLGLGWNADMMCGNFDIDTTVRNQLNGITDGFQQLMGNVIQSATGAV
                     ASLPAMIIQRANPQLYDLLTNGVLQGRLDFDKSLLNCQKMAEKMTDLAADSAWFSGAK
                     AENYQSLAASDNDAIRTDQKAAKEAAEKGKRWVGGEKRGGKGQLPIKVVHDATAAGWN
                     ILNQQPATSTTSLSSSECDGELCSTWTSPEEAAGWMTRVVGEQTISVAQVTDDPDARS
                     GAQAGIGLHPLIDEEKAKILPLLTDLVNGSQKPGFDNLAKASGGSMQLTRGVVEALRD
                     DPDVAVLTERLAGELALARVTEQALLARRTLLAGMREPNIANVKEAQESLGKTTAQLD
                     EELNQLKLELDFRQALTRNTASQILQRKQQRDQLQGQVVEVPDDSDSRLHNLNNPQPD
                     SPR"
     gene            26112..27614
                     /locus_tag="ACHKIR_001504"
     CDS             26112..27614
                     /locus_tag="ACHKIR_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012737263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG N-terminal
                     domain-containing protein"
                     /protein_id="HFO9989258.1"
                     /translation="MTANSYFEYVLTLLGWLVNNGVWQTLTATGLFAVPLLAKLVALW
                     LKARGQGADEGNVARLLVVWVEHSLYSALLVIFFAGVPFLNVDINTLTYDSQRTKQCG
                     WSVVQPGDSGYAPLINELSGQSASVPVWWYFTHALGKGITSAAVAVLPCKPDLRQLRF
                     EVQHTRLADPALAQELQDFVNDCYGPSRARLKRLSSDISEEQSREVDWVGSSFYLATL
                     GYYDRDHSRSPRAAWPYDASRDDGLSDTGGGGYPACKEWWSDGDTGLKNRLLKQVDTT
                     LWQQLQKLGQSKEEYEEAVLRSLVSPRNMQVSQGGLVYNGFGGSAGDNLSQTQLLSRV
                     GGIIGAGLSSYALFPAFDNVRQALPMVQAFLEMALVICIPVILLFSAWDLKTVITLSF
                     VQFALFFLTFWWELARWLDNWLMQMMYDSDTHSYFNLWGLQNTSDDLIVNIIMGVMFL
                     VLPAFWLGALTWAGIRIGAAVAGGMGSAVGDIRSAGEQVGKMIVSKTRIP"
     gene            complement(27759..27959)
                     /gene="glgS"
                     /locus_tag="ACHKIR_001505"
     CDS             complement(27759..27959)
                     /gene="glgS"
                     /locus_tag="ACHKIR_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006174923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HFO9989259.1"
                     /translation="MNRQDINALKNFDFLARSFARMHALGQPVDIDAVTGNMSDEQQA
                     WFRERYDHYRKQAERARVIELR"
     gene            28293..28583
                     /locus_tag="ACHKIR_001506"
     CDS             28293..28583
                     /locus_tag="ACHKIR_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9989260.1"
                     /translation="MSLFDKKPSLMPVVIAPDVWQSAVEFPADPQQVEERLGNLLLVV
                     LLTLRTAGTSRKKVTFTIYCQPPQGDMKVPVALPLSLNYHGHYVQVSMAEQA"
     gene            28649..29554
                     /locus_tag="ACHKIR_001507"
     CDS             28649..29554
                     /locus_tag="ACHKIR_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014908068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zincin-like metallopeptidase domain-containing
                     protein"
                     /protein_id="HFO9989261.1"
                     /translation="MDRSNDIYQKVTDQIIAELENGNVPWLRPWRDGEPPFPINALSG
                     RPYHGINVPLLWNSADRQSFASDRWLTFHQVSALGGQVRRGEKSSLAVLYLPRTQEET
                     DALGQPLLDKDGEPKVRHFGIIREFWLFNLSQCDGLPQALSEPFTRPATPVEMAEAIT
                     MNSGVTLRHRRQSKAYYKPATDLVMMPHPQQFESSEAYYATLLHELTHATGHASRLNR
                     PGIAGAERTEALYAAEELVEEMGSAFLCAHCGIQGRLQHASYIGYWLDILRQDKRAVI
                     RASGMARHASEWLLNAVPQPCQLSA"
     gene            29620..29991
                     /locus_tag="ACHKIR_001508"
     CDS             29620..29991
                     /locus_tag="ACHKIR_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9989262.1"
                     /translation="MGLDLFFFERKKNAPDESQYARVGYFRKVNPLLKWVDKHVGLVR
                     NQVLLEVTKANLQALLSDLQSLTPDNCGAVYPTIDGFFYGYTDYGEHYWKDVRTVTLW
                     VKETLETFDFERDRLMFYAWW"
     gene            30165..31754
                     /gene="mobH"
                     /locus_tag="ACHKIR_001509"
     CDS             30165..31754
                     /gene="mobH"
                     /locus_tag="ACHKIR_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobH family relaxase"
                     /protein_id="HFO9989263.1"
                     /translation="MLKWLKRPSQSQPPTPDATLPEGWFAPQSAEQLLATPLRQSCLH
                     QLRQLTPLPDTLFDSWILAAIHRFAALVQLLPASQSHHHSTPGGLLDHSLEVACNAIR
                     LRQGYLLPPEAGAEEQARQASAWTVAVVCVALLHDTGKIAVDMEIHQRDGTRWHSWQG
                     YLAQPYRWQYQSHGRDYHLHPAATAMLINQLIPSPLLDWLAGYPAAFASLIYQLTGHY
                     ERSGALAELVQQADKASVARYLGGDIKTALAQKPSSFPQQLLTALRSLVQNEYRLNNP
                     ECGSDGWLTEDALWLISKTTADRVRAWLLQHGVSSVPENNNRLFDELLTHQLIVANED
                     KAIWRCIIRSDKGWSPVEPLTLLRLTPSVIWGQDDQRPACFDGDVTPVIEAEPAPELS
                     APTQPLPEIADDPRLGDNNNFLQWLKNHTSPSATVNTRNGKVHVVENHLFLISPGIFK
                     TYAAETTGDSGDGWKLAQRMFQESGLALRCNDDSFIWICEVRVPQKKSSQLKGFLMED
                     PKQVLGEKVPVNNPWLKLLRE"
     gene            complement(31810..32463)
                     /locus_tag="ACHKIR_001510"
     CDS             complement(31810..32463)
                     /locus_tag="ACHKIR_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFO9989264.1"
                     /translation="MKKKLPDQLSRECIIAAMRAYDEGVRHQFSESRLYDVVFEGRSY
                     PPKAIVGIAASNMTGVEFSPEDFSGGIKSKCVKLLTDQGFQIVEKHLGNEQPAISIFP
                     DELPPQSVFTEGAATQVTVNRYERDRKARQAALAWHGCLCKVCGIDMMKVYGEIAKGF
                     IHIHHLIPLSDIKENYSLDPKKDFIPVCPNCHAMLHRRNPPFSPEELKSIIEYHAAL"
     gene            complement(32575..32841)
                     /locus_tag="ACHKIR_001511"
     CDS             complement(32575..32841)
                     /locus_tag="ACHKIR_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005289972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9989265.1"
                     /translation="MSPRTHRQLVSVEVMWPAQTLPLPLQQVVEALNQGETPDQIIIR
                     MNQRGLLAWREDASAQDTHDIFQVRLDNQNEARFLCRYVTLPLH"
     gene            complement(32968..33309)
                     /locus_tag="ACHKIR_001512"
     CDS             complement(32968..33309)
                     /locus_tag="ACHKIR_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012776626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO9989266.1"
                     /translation="MNVTSPKMLSQAIRDARKQRNLTQRETAASMGMKQSTVSEFENH
                     PEGTSLDTLFKLLAALDLKLQVVSWRGKKTLLMAGSRSGDLWKYEQKGHGAIRTEPPF
                     SLAYHLDFQST"
     gene            complement(33720..34208)
                     /locus_tag="ACHKIR_001513"
     CDS             complement(33720..34208)
                     /locus_tag="ACHKIR_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO9989267.1"
                     /translation="MAKKTAPLVPGTNQLLAEFGERLKLARLRRKLTAKRVAERAGMS
                     PMTLRSLESGGTGVTIGAYLSVMQILGLEKDLNKLAAEDEQGRQLQDSRLINKTRVSR
                     PAAISHTNRHTGNVIKNDAFDKQFSTSQEKSIDSEVKTEVVTSASLAALLKPIKKPRK
                     GS"
     gene            complement(34516..35325)
                     /locus_tag="ACHKIR_001514"
     CDS             complement(34516..35325)
                     /locus_tag="ACHKIR_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009111697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFO9989268.1"
                     /translation="MTNIFTLMNPISSATETLLSQINRICTAQGIPFFIAGATAREIL
                     LHHIHSRSIGRRTRDIDIAVFVDSWPRFDALKNAFLAEGAEVVQGNAHRMIVKGIELD
                     IIPFGGVAEGNQIAWPPEREIIMAVDGFAEVFSHTVVVRLDNSENIPFCSLPGLALLK
                     LFAWRDRGHGSTKDATDLFKIICEYGVIENERIYESPVEGERLNWDPIRMGAFLLGSD
                     IATISEHSSLQDLLHLDKERLIDVIVRQHDVGDAGIEQIINDFWDGMASDG"
     gene            complement(35279..36277)
                     /locus_tag="ACHKIR_001515"
     CDS             complement(35279..36277)
                     /locus_tag="ACHKIR_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009111696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin"
                     /protein_id="HFO9989269.1"
                     /translation="MKEEQLLSDAIENLPKGFHLQYEHSGRHGKHDAWGKLTGPDGES
                     VIFALEVKFIHRKESLMAVREQMVRFPADFPVLLVCNRLTSALAEYCADSQINFIDTA
                     GNARIQVPGLYVRIEGRYEKKPVSASSRFAEGVMKLLFVLLSSPETLNETYRSLAGKA
                     GISLGMVSKAFDYLEAQRYFRKSKKGRRLINEGELEALWLKDYATALRPKLDSLSLSA
                     PVSWDELTLASGEYWSGEIAAAKLSGGYLIPENGVIFTSHPLLQRRKELGLKPVPGGK
                     LQLIAGFWGNFVLNHKAEAMLCVAELLASGDDRNWETARIINDKYLHLNESDLFSY"
     gene            complement(36905..38152)
                     /locus_tag="ACHKIR_001516"
                     /pseudo
     CDS             complement(36905..38152)
                     /locus_tag="ACHKIR_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008322911.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(38375..38459)
                     /locus_tag="ACHKIR_001517"
     tRNA            complement(38375..38459)
                     /locus_tag="ACHKIR_001517"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(38423..38425),aa:Leu,seq:caa)
     gene            38655..39674
                     /gene="ahr"
                     /locus_tag="ACHKIR_001518"
     CDS             38655..39674
                     /gene="ahr"
                     /locus_tag="ACHKIR_001518"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HFO9989270.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTILNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALAGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSVSGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            39804..41306
                     /gene="yjgR"
                     /locus_tag="ACHKIR_001519"
     CDS             39804..41306
                     /gene="yjgR"
                     /locus_tag="ACHKIR_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like C-terminal domain-containing
                     protein"
                     /protein_id="HFO9989271.1"
                     /translation="MSEPLLIARTSDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGVAEEGTSSEKLLARLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLPWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(41425..42507)
                     /gene="lptG"
                     /locus_tag="ACHKIR_001520"
     CDS             complement(41425..42507)
                     /gene="lptG"
                     /locus_tag="ACHKIR_001520"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFO9989272.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(42507..43607)
                     /gene="lptF"
                     /locus_tag="ACHKIR_001521"
     CDS             complement(42507..43607)
                     /gene="lptF"
                     /locus_tag="ACHKIR_001521"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFO9989273.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            complement(43670..43720)
                     /gene="ytgA"
                     /locus_tag="ACHKIR_001522"
     CDS             complement(43670..43720)
                     /gene="ytgA"
                     /locus_tag="ACHKIR_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtgA"
                     /protein_id="HFO9989274.1"
                     /translation="MSIIILATISTTQENK"
     gene            43874..45385
                     /gene="pepA"
                     /locus_tag="ACHKIR_001523"
     CDS             43874..45385
                     /gene="pepA"
                     /locus_tag="ACHKIR_001523"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HFO9989275.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYRGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            45519..45962
                     /gene="holC"
                     /locus_tag="ACHKIR_001524"
     CDS             45519..45962
                     /gene="holC"
                     /locus_tag="ACHKIR_001524"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HFO9989276.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            45962..48817
                     /gene="valS"
                     /locus_tag="ACHKIR_001525"
     CDS             45962..48817
                     /gene="valS"
                     /locus_tag="ACHKIR_001525"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFO9989277.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EDEVRKENNLSADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(48871..50067)
                     /gene="yjgN"
                     /locus_tag="ACHKIR_001526"
     CDS             complement(48871..50067)
                     /gene="yjgN"
                     /locus_tag="ACHKIR_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjgN family protein"
                     /protein_id="HFO9989278.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLVGCV
                     LTLLLLVFLIFMAAKGLRYQALMTSLNGVRFSFNCSLKGFWWVTFFLPILMAIGMGTV
                     FFISTKMLPANSSSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTTYCIKYAILAFLALLPFLAVAGYIIFDQILNAYDSSVYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGVM
                     PSLPFL"
     gene            50260..50763
                     /gene="yjgM"
                     /locus_tag="ACHKIR_001527"
     CDS             50260..50763
                     /gene="yjgM"
                     /locus_tag="ACHKIR_001527"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO9989279.1"
                     /translation="MNNIAPQSPVIRRLTLQDNPAIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYEGEVVGGGGIAPLTGSEADICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIGLYEHLGFQHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(50809..51225)
                     /gene="rraB"
                     /locus_tag="ACHKIR_001528"
     CDS             complement(50809..51225)
                     /gene="rraB"
                     /locus_tag="ACHKIR_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HFO9989280.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            51387..52400
                     /gene="argF"
                     /locus_tag="ACHKIR_001529"
     CDS             51387..52400
                     /gene="argF"
                     /locus_tag="ACHKIR_001529"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFO9989281.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     LNN"
BASE COUNT        12519 a        14219 c        13502 g        12182 t
ORIGIN      
        1 ttttttcgat gctgtaaacc tcagttcaac cttagggatt atattctttt ttattttatt
       61 atcgatatca ggattcagcc taataatttt catctcatct tttattttaa tacttattta
      121 ctctggatat gaaaacaagt taaaggtata ttctagcatc acccatcgga tttctactgt
      181 ttgctatcag aatagcttat ttatatgctg cctgctaatc attgcttatt acatttatta
      241 catgacaaaa tggaatggct acataattac agcctttagt attatttcca tatttattca
      301 cagctatgtt acatgtgcga ttcggctatt tagaaaacaa aaatctcgct taaataatac
      361 agtgcgatac gaaaaactat ccagaaagaa aggattcaac ttctggttat ccttacaact
      421 tataatacct ggagtagtac aaatattacc aatgatgttt ctgtttaacc aacttaattt
      481 cagtgagggt actagtgact ggtatgagat gagcatactc attgccataa ctgtagttat
      541 tgttacaatt ggcattctgc ctggtatcat acatattaat gaacggcaaa acggcaataa
      601 aatgacagga attattgttg ttttaatatt tatacctgtc gcaaccgctg cacttagtgt
      661 ttggtacaga cctattccca acatgatagc taatatgaca atgaatcttt cagggattag
      721 tgaccagaga acccatgaat actatattga aagagcaact cacccagcag gtatgtttaa
      781 tggtaaaata tggaatactc gatactataa aaacatccct gatagattct tcattactgg
      841 agttaacaca tttactttag ggaatataaa actcatctgc ccaacagcaa tagtcaaagc
      901 aagaatagaa agtttgaaat tcactgtaaa tgacattgac gagtacgaac aaaaagggaa
      961 aaaactaaaa aagacagcga tgaaatgtat tccctttgat aaaaatgata tacatacctg
     1021 ggactcgcca ttatctgaac caatttatta cgagaaaatc aaacagacca ttgacaactc
     1081 tatgctaaaa attcttcatg tagtgaaatg attttttagc tgcttcacct ctgaagctct
     1141 cctactttgc taatcattga gtataaggag agcttcaaat gcagtcccag acagcactat
     1201 tactgttaat aatacctttc ccggtactgg taatcgcctc cccggaatcc acccagctcg
     1261 ccctcgcgct gaagcaactc gacggcgtta aaacctcgct ggagcacgcc cggaccagcg
     1321 ccagcaacga tccgcaggcg cgctacttct tcgactactt acaggcgcag caggacatct
     1381 ctgtcgttga acaaggcatt cgccgctatc ttgccccggc tagagcacag ccgcgtgcta
     1441 ttcagcccct gagcgccaac taccagcagg agaatcccga tgactgactc gcaactcgcc
     1501 gcctttcagg ccgccagcct gagcaaaccg gagaccgtca gcctgctggt gctcggcctg
     1561 tttaccagcg tcctgctgct gtggtgtgcc tggacgctaa tggtcgcctg gcagggcgtc
     1621 tgccgccagc gtctgagctg gcagagcttt ggcgcggtgg cggcccattc actgttactg
     1681 gtgctagtca gcttctggct ggtactgagc tgaggaggca tcatgcgctg gtttctgtta
     1741 ctgctgttcg ccacatttaa cgccctcgcc gacctgccga cgatggaaga tccgtcacgc
     1801 ggtcagggca agggcattat ggagacgctg caaaactacg gttttgacat cgtggtgctg
     1861 ctggcgctgg ccatctgtgc cgtcggtttt ctggtagtgg cgaacaactg tattgctacc
     1921 tattcggaga tccagagcgg acgcaagcag tggaaagatc tgggctcgat ggcaggcgtc
     1981 ggcagcattc ttctggtggt catcatctgg ctgctcaacc aggcttcgga cgtcctgtga
     2041 tatggcaacc atcccctttc tccccgaccg cctcaaccgc gagcccgtgg tctggcgcgg
     2101 cctcaccacc agcgaactgt ttctggcgct ggctctcggt cttggcggcg gctgtgcgtt
     2161 cggcattctg ctggccctga taacccacta ctggccgttg atccccggtt cggcactttc
     2221 tggcgcggca ttactgattc agggtggtgg gcggattctg gcccgcgcaa aacgcggtaa
     2281 gcccgacacc tggctgccgc aggcgctcct cgcctggctg gagcgtcatc atcttcgcgg
     2341 cgcacaactg atccagcaca gcgccgtgtg ggtggtgcgc aggagtccac gatgagccgc
     2401 tttcgccatg cgctggctga gcgcgataac catatcctga cgctgcgggc ggtgattgtt
     2461 ctgctgtgcc tgtgtctggc gggcgtcggc tacggctggt acagcgcgcc gcaaaagctc
     2521 accctctggt tcccgccgga tctgcgcagc ggcagcaccc gcccgtggtg ggaagtgcct
     2581 cccgccagcg tctacgcctt caccttttat atcttccagc agcttaaccg ctggccggat
     2641 aacggcgagg aggactattc ccgtaatctt cgttcgctgc gtgcctacct gacgcccgcc
     2701 tgttacaccc ggctggagca cgattttcag gtgcgtcgca acggcggtga actgcgcgac
     2761 cgcacgcgcg gcgtctatga actgcccggc cacggctaca gcagcctggt gcatgagggc
     2821 gagaagcgcc ccttcccgcg cgtggaggcg ctgagccggg attcatggcg ggtaacgctg
     2881 gatgtggcgg tggaagagta ctaccagaac gagccggtga aaaaagcgct ggtgcgctac
     2941 ccgttgcagg tggtgcgcta cgccatcgat gcggagaaaa atccgtgggg actggcgatc
     3001 gactgtaccg atgccgcgcc gcagcgtctg gtggcggagg agaaaccatg aagccagtcc
     3061 tctttacttt attgttcagt ttccccgcct tcgccacgga agtggtgcag tgggaaaaca
     3121 tcccgctgcc cgtcacgctg catgtcgggc aggagcgaat tgttgacgtg ggtaaaccgg
     3181 tgcgcattgg ctatcccgcc acgctggaag gcaaagtgcg tctgcaaagc gcaggcggca
     3241 aggtcttcct gctggcgaac acggcgttcc cggccacccg catccagtta cgtgataccg
     3301 gcaacggtga gctgatcctg ctggatattc atgccaccca gggcacttcg acacttgaac
     3361 caatgcaaat tagctatacg ccgcagacgc ctgccacagc aaaaacatcc gtcccggtga
     3421 cagcatcaac agaacctctg cccgtcttgc tggtgcgcta cgccgcgcaa aatctgtatg
     3481 cgccgctgcg caccgtcgaa gccctgcccg gcgtcacacc tgcgcccgtc cggctggcga
     3541 aacgcatcac cactttactg ccgcagcagc cggttaccgc caccccgctg gcgtcatggc
     3601 aggtcaacgc caccaccgtc accgccatcc gcctgcaaaa tcacagcgga cagcgcatca
     3661 cgctcgatcc gcgcgagctt caggggcagt tcaccgctgc cgcgttccag catgactggc
     3721 tcggcccgcg tggtctggca gaagatacca ccgtggcgta cctggtgacg gaaggtccgg
     3781 tttcccgcgc cctgctgccg gagcccaaac catgatgccg cgccagagcc tgctgctgaa
     3841 aatcctgctg ccgctcatcg tactggtaac ggtatggctg ctgctgcgcc cggccagcaa
     3901 aaacacgcag gagacttccc tgtcccgcac cgtggcggcc ttaacgccgc aggaactgcg
     3961 cgatctgggc atcgacggcg acacgcccgc cgacaccgtc gccacgctgg tcggccagat
     4021 gaaacagtac cgccgcgaac ttcaggcaat ccagacaaac aacgaaagcc agcaggcgcg
     4081 cgaaaaacgg cttaacgcac agcaggccac gctggcctct cagcttcggg aagacatgcg
     4141 ccgccagcag gaccagatgc gcgatgaact ggtgaaaagc cagcgcgggc tgctggataa
     4201 gctcgaccgt ctgatggcca agcgcccgga cgatatcccg cccggtctgg ggctgacgcc
     4261 cggcgaacta cctgccgacg ttcaacccga tatccgctgg ctggagccgc tggatgcgcc
     4321 tccacctgtg gatgaaaagc tgctgcgcgt ccctctgaaa gcggccgaca aaccaccgcc
     4381 gaaacctgac gtgaaggtct acaccctgcc gcagaacgcc acgctgaccg gatcggtggc
     4441 gatgacggcg ctgattggcc gcgtgccgct taacggcacc gtcagcgatc cctacccgtt
     4501 taaacttctg attggcgagg acaacctcac cgccaatggc gttgagctgc cggacgtggc
     4561 gggcgcggtg gtctccggca tcgcctcggg agactggacg ctctcctgtg tgcgcgggca
     4621 aatcacctcg atgacctttg tgtttcacga cggcatcatc cgcaccgtcc cgcagggcgg
     4681 caaatccggc aacaaacagc aggagatcgg ctggctttcg gataaccagg ggctgccatg
     4741 tattcccggc gagcgtaaaa gcaatgcgaa ggagtacctg acctcgcagt tcctgctggc
     4801 gggttccggt gcggcggcgc aggccttcgc caatggcgaa agcaccacag tggtcgagga
     4861 cggcagcatc accagcgcag tgacgggcaa caacggtcag tacgtgctcg gccaggctat
     4921 tggcggcggg ctgaaagaga ccgccgactg gatgaaacag cactacggcc agatgttcga
     4981 cgccgtgtac gtgccgccgg gccacaaggt ggcggtgcat ctcaccgaag cgctgtctat
     5041 cgactacgag ccggaaggcc gtcaggtgaa ataccagcag gccagcacac cgggaggtct
     5101 ggaatgaaat ttaccatcct tatacttctg ttgttactgg ccggatgcag cacctcgcag
     5161 gaggaactga tgcctgtcga ccccaatgcc agcatgctga cgctgtggca gcagcaaagc
     5221 ggcggcaacg cggctctgct ggatgcccgc gcccagctac gccgcccgcc agatggtccg
     5281 gtggcgcagc ctgaggcaga cactatccgc agccagtttc cgcgcctgcc caacccggat
     5341 atggtgcttt acgtcttcca gcacctcaac agcaacacgc cggtacccgg ctacagcacc
     5401 gttttcccct tctatacccg tccgcagtat gcgttgccgg gcgaacgcct gacgccgcca
     5461 tgaatgaacg gcgcggcctg actgaacagg cgctgaatac cagctaccgc aagggcccgt
     5521 catttgtcga ctggctgccg tgggtggagt ttttgccgga gtcgcagacg ctgctgctgg
     5581 aagatggccg ctcggtaggt gccgtatttg agctatatcc cgttggcacc gaaggccgct
     5641 caacggcgtt tctggaacag gtccgcgata ccgtcaccga tgccttacag gatgcgtttg
     5701 atgagcatga gcgcgcaccg tgggtggtac agtttttctg tcaggatgag gacgacagcg
     5761 aggcgtggct ggcatcactt gcctcttacg ccgcaccgga aatccgcgac tccgccttta
     5821 cccgcgagtg gctcacacag atggcaaacc atatggtgaa tctcacccgc gacgagggac
     5881 tgtttctcga taacaccgtc acccgtaccc gctggcgggc gacgtggcgg cgcacgcgga
     5941 tggtggtgta ccgatggctt cctggacttt caccggatga accgtcgccg gaagagacgc
     6001 tgaataatat ctgcgaacgc cttaccgccg ggctaaaagc ggcgggtatc agcgctatcc
     6061 gccagaatga gcaggacatt caccgctggc tggtgcgctg gtttaatccc gcgccgcctg
     6121 ctgaggacaa aaaagccttc tggcagcagc ttgacctgcc ggaaactgaa ccgctgccgg
     6181 tcctggccga tttcgccgaa cgcctgctgg cgagtgaacc ccgttcggat gcggcaaacg
     6241 gcgtctggtg gctggataac cgcccgcata aagtggtggt ggttgaccgc ctgcgcaaag
     6301 cgccgccgcc cggcctgctg accggggaaa ccgcgcgcgg agacggcatt aacgccctgg
     6361 tcgacctgct gcctggcgga accatcatga acctgaccgt ggtggtgtat ccgcaggaca
     6421 agctggagca acacatcagc cgcctggccg accgggcgat tggcgacaat accgattcgg
     6481 aatacacccg ccgcgactgc agcgaggtgc gaagcttcct gaaggacggc cataaactct
     6541 accgcagcac gctggcgttt tatctcagcg cgccggacac cgatatcctg ttccgccagc
     6601 agcgtcagct acagagcctg ctgctcaacg ccgggctggt tccggtgcag gagcagcacg
     6661 aagtcgcccc cttaagccac tggctgcgct ggctgccgat gtgcttcgat cccctgcgcg
     6721 acccgcgcgg gctgtatacc cggctcaact ttgtgcagca tatcgccggg ctgctgccgc
     6781 tgtacggacg ggaaaccggc accgggcatc cggggatcag ctacttcaac cggggcggca
     6841 cgccgctgtg cttcgatccg ctgaacaagc tggatcgtgc ccagaacgga caccttttac
     6901 ttcttgggcc aaccggagcg ggcaaatccg ccaccctcaa cgcgaaaatc gcgcaactga
     6961 tggcgctaca tcgtccgcgc ctgtttatca tcgaggccgg aaactcattt ggcctgatgg
     7021 ccgactacgc cgccgcccac ggcctgacgg tgaataaaat cagcctgcgc cccggttctg
     7081 gcgtcaccct gcccctgttc gccgatgcgt ggcgactggt ggaacaggca gttaccgacg
     7141 ccgaaccgcc agaggacgaa gagagcgacg gcgatgacca gcgcgacgtg ctgggtgaaa
     7201 tggagatcac cgcccggttg atgattaccg gtggtgaagc gaaagaggat gcgcgcctca
     7261 cccggccgga tcgggcgatg atccgccagg cgattatcgc tgccgcgcac acctgctacg
     7321 aggaaaaacg acagacgctg acccaggata tccgtgacgc actgttcgcc ctttcccgcg
     7381 acaacagtct gccggaagcc cgccgccaga gggcgtatga aatggcggaa tccatcaacc
     7441 tgttctgcgc cgggtttgaa ggcgaactgt ttaaccgtga aggccagatg tggccggagg
     7501 cggatatcac cctagtggat ctcgccacct ttgcccgcga aggctatgaa gcgcagcttg
     7561 ccatcagcta tatctcgctg attaaccata tcaataacct cggcgagcgc gcccagcatc
     7621 tggcgaggcc tatcgtcaat atcaccgatg aggcgcatat catcactgtt aatcccttat
     7681 tggcgcgctt tctcaccaaa ggctcgaaga tgtggcgcaa gctcggtatc tggctgtggc
     7741 tcgccaccca gaacctggcg gattttcctg atgaggcgaa gaagctgctc aatatgatcg
     7801 aatggtggga actactggtg atgccgccgg aagagatcga gcaggtctgc cggtttaaag
     7861 cgcttacgga agaacagagc caactgctgc gctcagcggt aaaatcaccg gggcagtata
     7921 ccgagggcgt ggtgctttca ccgcgtatag aggcactgtt tcgggtggtg ccgccagcgc
     7981 tctatctggc gctggcgatg acagagaaac atgaaaaggc cgataggatg cggattatga
     8041 aggagacagg gtgtagtgaa gtggaggcgg cgataaccct aagctcggta atctcgcaat
     8101 aaaaatatgc taagctattc gcattaatgt taaataactg gaaaaacctt atgaccgcaa
     8161 ttgcatgttg gtttaataga gaggaaagcg atagtatttg ggcagtcagc gactcaagaa
     8221 tcactcagca aaatagcaga ctaactgacc attgtccgaa attattctca atccccgtat
     8281 cagttattcg ctcttcggac gtttatcgaa ttcacccgca aaaaatattc gaatttggct
     8341 tcggatttgc aggaagtaca atgattggaa ttaatgtcaa agaaatgctt gcagtatcat
     8401 tagcaagact tcatgagatt gcgaacaata atccagaaca acaaatatcg accgaagcat
     8461 tcccaagtct taatgaaata gcttcattag caaaaaatat tgccgagcaa tatatgtctg
     8521 atatcggcca atcctttccc caagcagtaa gaatacagat gataattttt ggtttttgct
     8581 ggagaacaca aacctacaaa attataaaac tcaataactc tattgagcac ccagccaacc
     8641 tccagattga agatattcaa aatttatcaa caggcagacc tgttttatta ggtgataact
     8701 tgcatgaact taatgagttt atagagaata ccaggcaaca atttactcct aacacaataa
     8761 attggtggcg taccccgttt attgctttga ataattggat taaccaagaa acagtcaata
     8821 ctattggtgg atacatccaa atggtaaccg cgcacccact tggtactcga atatcatttt
     8881 tgaccaattt gaatgaaaat attatcagaa caagctatgc aggaattaac actactgagt
     8941 catttggacc aactattggt ggttttatcc taatgccaat ggatgggatg tcactcccag
     9001 atgaaaacgg ctgggatgtt ggaaatcaag ttacccgtac tgctgcggat agagttgcta
     9061 ctaatagata aacctatctt tcaaagttga ataattcttt gatgtccatg tccaaccctt
     9121 ttcctaaaac acttatcacc tcaagggttg gattccttac tccccgctca ataccgctca
     9181 cataagtacg atcgagacca cacttatccg cgaatgcttc ctgagacagg cccgactgga
     9241 gtctgagttt tttgagatgt gcgccaaact gcgcctgaat actggatgta tttttcatcc
     9301 agataaaatg aacttacgtt gtctatcaga ccacggacta tgagtcacac ttcgttgatt
     9361 cggcgtcctt ttctgcgcca tcgtcaaaca taaataaatg gctaagatcc atccccagac
     9421 cgtccgagat tgcacaaatg acaacaagcg tggggttcct tactcctcgc tcaattccac
     9481 tcatataggt gcgatcgata ccgcatttgt cggctaaagc ttcctgggat aggtccatct
     9541 cacggcgtag agccctgatt cttttaccca gtaccttttt gattgatccc gtcttttcca
     9601 tctagccagc atcaatcttc gttgattatc agtccacgga ctatgagtca cataaaaagc
     9661 gtgctagcat acattcgtga ctcatagtca acaatagagg taaacggaat gactaaaaaa
     9721 gactggcacc ccgccgatat cattgccgcc ttacacaagc gcggcaccag tctggcgaaa
     9781 ctctcgcggg aagcgggact ggcctcgtca acgctggcga atgccctgaa ccgaccgtgg
     9841 ccgaaaggtg agtggctgat tgccattgcg ctgggaaagc atccgtcaga aatctggccg
     9901 gaacgttacc tcaatacggg aaataaacgt gaaccccgta aaccgcttca cctcatgaca
     9961 acaacagaaa actccgggac ataacgaccc gcgctgtgag tggcaggacg cattaatcaa
    10021 cgacacctga caggagagtt gtttatgcac accgaaccac acccttgctc catcgctctt
    10081 acaaccggag gccaccttca cccggcctcc cggactgaag gctttattca cctattcagg
    10141 ctacatgcct gctcacctac accaggccgt actctcatgc ccgacaaacg ggagaacgag
    10201 ccatgatgac ctactgcgca cgcatcgttg ctatcgggct gtttgtcgcc gacggactgg
    10261 cagacaggac gcttatcacc tttgacagca acggaccgaa agactgcctg gactattccc
    10321 tgtcgctgga accctcattc cgcgaagacg acctgctgat cctgcccggc gatcggctgc
    10381 tgctggcgag ccatgactac ctggtgaccg ccgtgggtaa agaggcacag cagtctttgt
    10441 ttgaacttgg ccacctgacc atggtgttta acggcagcct gaatgcctgc cattcgggag
    10501 ccgttcatct ctccggccca atccctaaac ttcacgacct gtacggtgaa ctgctgattg
    10561 aggagggacg gccataagcg ctgccttgaa tccatccaca acaacaaaag agaagacatt
    10621 atggaactca acagaagtct gaaagcccgt cacctgacga tgatttccgt gggaggcgtg
    10681 attggtgccg gatttttcct cggcgcaggc gcggccattg cccgtaccgg gccgtcggtg
    10741 gtgctggcct accttatcgg cggtgtcatc accctgttca ttatgctgtt gcttgccgaa
    10801 atggcggtcg ccaggcccgt ctccggttcc tttgaatcct atgcccgcac cgcctttggc
    10861 ccatggtgtg gctttatcac cgggtggacc tactggctgg ccttcctgat tggcccggcg
    10921 tcggaggcca tcgctgcagg caccttcctg aacctgtggt ttcccggagt ccccgtctgg
    10981 ctgttttgcc tggtgatcgc ttcgtcgctg actgccatca acatggtcgg cgttcatttt
    11041 ttcggtgaag tggagttctg gctgagcctg gtgaaagtcg tggcgctgct ggcctttatc
    11101 gtcgcgggct gctacagcct gggactggat aacgcctcac ccgccagcat cagtcatccg
    11161 accgacagcg gcagcttttt tgctggcggg ctgcccggat ttctcggcgc gatgttaatg
    11221 gtgattttct ctttcggcgg gacagaggcc atcggcaccg ccgcgggtga aagcgagaac
    11281 ccacagcgtg atattcctcg tacgctgcgt ggcaccgtgg tgcggatcct gttgctgtat
    11341 gtcggctcat tgaccgttct gctgctcgtt ctcccctggc agcaggcagg tgtcagcagc
    11401 agcccgtatg tcgaagcttc cggcattctc ggtggccata tggcgcggca tatcatgaac
    11461 tttgtggtgc tgaccgccgc gctctcctgc atcgataccg gggtttacgc cacctcgcgg
    11521 atgctgcacg ccatggcgtc tgacggctat ttcccggcct ggtttgcccg cctgcacccg
    11581 gcacataaaa caccggataa cgccattctt gccagcagcc tggtgctgtt taccggcgtc
    11641 atcatcgcca ttctctcgcc agatgcgtat gtcatcctcg ccgggatttc cggattcggc
    11701 tttctgttca cctggctgat gattgcctta agccagccca ccctgcaccg aatagccctg
    11761 cacgacggaa cgctgaaata caaggctccg gccgcccgct acatccagcc actcaccgtg
    11821 gggctgattg cgctgatcat ggccgggcaa tttttcaccg acggcggcgg cattaccctg
    11881 gttaccgtcc tgatctggct ggccctcgcc agcgcgtact acatctttgt cgtccgaaaa
    11941 aacaggaggc tgtccgatga aaaaagtcat gctcaatgaa ctgactggcc gggaaatccg
    12001 cgagctgttg cagtctggta aggaactcac tgctatcgtc aacttcggca gttgtgagaa
    12061 ccacgccgat cacctgccac tcggccccga tatgtttgta cccgaaatcg tcgcctgccg
    12121 ggtggcgcag cgggtggata atacccttgt gctgccctgc acgccgttcg gcacatccgt
    12181 tcactacaat gcctatccac tggcggtcac cctacgctac gagaccaata tcgcgctggc
    12241 ggaagatatc ttcatcagcc tgctgcacca cggtattcgc cggatcctgg tgattaacgg
    12301 ccatgacggt aatattccgg cgattgaaat cgccgcccgc aatgtgaagg cccgacatac
    12361 gcaggcagcg attgtcgtgg ttccggcgtg gtgggatctg gtgggacatc atctcgggaa
    12421 gggatttttc gactcatggg ataacaccgg actaggacac ggcggcgagg gcgaaacgtc
    12481 cgtcatgatg gcgctgattc cggagctggt ggaactgaaa tatgcgcaga aacagatccc
    12541 ggtggaggtg ttcgctgatt atggcgtgaa ccggatctgg gatatcgggg aaatcagcgc
    12601 caccggagca accggcgatc cgcgctgcgc cacggcggaa aaaggccagc ggatcctcgc
    12661 ctgtctggtg gatgtgcttt gcgcgttgct gagaaagctg gatgcagcta actggcaata
    12721 tgcgttaaat aaagacgcaa acatgtaatc gatatgctcg ggcaacctgt ggagtggctt
    12781 ttgcctttcc acaggttacc cggaagcgat atcccaaccg ggtaaccgat tggtgttagc
    12841 ccagaatcta ttatacttca atgagtaata ttattgctga gatgaatgac agttcaggtt
    12901 tagtcacctt accaccaaca aattctgcac atttactcct gagatttgct aattcatctt
    12961 ttaatttttc gttgctgtgt tgcttaagat atatttgtcc tcttagctca acttcagtac
    13021 ttgccttcaa aaattccttt cggctttgat aggtgggatc gttatcgaga ttttttggta
    13081 tagttattaa acatttctga aatgcgatta aagcttcgat taggagaagt ctagagtttt
    13141 ctttgtattg aggcttccac tgatgcaacg accgccaagc aagtagtaaa cctatcgttg
    13201 tcacaacaaa cgtcaaacag gcagcaatag cagcaacccc cgcccatttt gcagcttgtt
    13261 gattagccac caatgtgctc aagcttatga aatccgcatc tatatgcact atttttacct
    13321 cagtaagaaa agtggatgta aatctacttt ttcatttttg aaacagcaac ttgattttaa
    13381 gccatcgtcc tttgagcaag attttccgca ccagttgaat cacgtcaacc accagcacgc
    13441 cctcctctct cagtgcatcc catcataatg gcaatctgta aaacagcagt ccttgcttta
    13501 aaaatgtgtc attcctcaat tgtctggcca agcttattca aatcatccag ccccgagatt
    13561 tgcttgagca cagaaaggta ctcctgatgg ttatcataaa gccagttaag tacctgaccg
    13621 ttcgccagca gacgttcaat gtgattttta atgataacca gccgaaggtt attctcagca
    13681 tattcatcct gaagatgctg cgtttccacc tgaacggaag ccatttcttt ttccagtctt
    13741 tcgagatttt ttctgatatc tgtttctctg ctggcatctt ttttaggcgc tacaagatgt
    13801 tcaggggcaa cggcatgcag catcgacatc gcaaatttgc gggtgaaatt attaatactg
    13861 atcatggtgt taaccatttc tatctgctta tagaacttta gttttttcaa cacagagaaa
    13921 atggtctgcg gtacattttt ttctgccagt aacgccatga cttcaggggc aatgccatca
    13981 gaaattttca gtttaccaat cagcgtatct ggcgtgacac ccagtacggc gcatagtttt
    14041 tcaactgaaa cgccagattc aaccgcttta agcagcatgt tttgggcctg cacaatggta
    14101 aggcgactaa cacgtttgtt atatgaatat gtatcctcca caggggatat cagacaatga
    14161 gcagtagtta ttccgagttc tttcaaaact tcaacgcgaa gatgtccatc cagaatgact
    14221 ttatcattgg cgtcattgat ataaaccaca atgggttcaa ttaacccaat ttcatcgaca
    14281 gaggctttaa tttgtttgta cttcgtactg aatttaacat taaccggcag cgttctggca
    14341 tacaccagtg attccacctt aaaggtataa aatcgggagt caaaacattg tgaaatcatc
    14401 ttatttctcc cccgatacat cactgccatc cagtaaagaa ggaatatcgt ggatattatt
    14461 actgtgcagc aatgtaacaa aatcattatc tttaagtaaa taaccaaata tttgctttac
    14521 tgccagaatt ttctctttaa ccattgtgga tttcagcaga atggcttttt gctcattaat
    14581 gttttcctgg tagacattag ccagttcttc cggggtcagt tttttctgtt ttttcccagg
    14641 aaaaaaagcc gtactaatcg tacctttatc gcctttctta cgttgttcaa gtatgcgcct
    14701 gatcttatta atttgcccac ccttgatatg tccttctttc agggcgaccg tcagcagttc
    14761 ctgagtttcc tcatctcctg accgggatat ttccacagca agatacaagg gaatatcgcc
    14821 agtctcaaat gcccgcaaaa gatgcttctc tccggatgac aacagagtga tcaccccatt
    14881 tacccaactc gaattatacc caagccgcaa gcctatttca cgatcggtca ggccactggt
    14941 cttcattctt ctgatatctt ccattagctc tgccgcccgg ggattacgtc ttgcgatgtt
    15001 ttcagccagg ctcatcaggt gcgctgtctc ttcatcaacc tgcttgatga ttgcaggaat
    15061 tttcgtttca ccatactcaa tatacgcttc aagtcgtccc tgaccacaaa tcagggcata
    15121 tttatagttt tcattattca ctgggcgcac gctaacaggc cgtttcagac caatggtttt
    15181 tatgttctct tttatttcat tatgaatgaa tttattcctt ttccgtggat ttgcgatata
    15241 aatatcactg atatcgacaa gttctatctg ttcatcaaaa gatttcatta tttactctcc
    15301 agaccagttc gttttacaag attttccaga catgacaaat catcaaatcg atatagttcg
    15361 aggataaaac cattgttctc atgaagcgtt aaatcgttat acaacacatc aatagccgga
    15421 aacataaagt aatcaaccac ggagttattt gtgctatcca accgtggaaa gatcatgaca
    15481 tcacaataat gggatacatt aaattttatc ttccaacgaa gcttacctga cgacaaacgt
    15541 tggcagcgaa ccagattagc ggaaacaaaa aactcatcat taatatataa tgaaccatcc
    15601 tgatgcatct ctgcccaacc gccatatcgg tatatatcct gcaccaaatt atccatgaaa
    15661 gtgagatggc attctgcaat ctgctttttt atttcaagat aagagtaatc atggcgcggg
    15721 tgataaccaa taagggtgta agccctgact aacccaccaa accgtgaacg aaaaatgctg
    15781 ctcgatggaa acagttcctc ttcatcaatc acaatcccgg ataatctgcc acgaagcttc
    15841 agcagatggc gtagtttatc cagcagttca tcatcactgt actgaacggc cctggcttca
    15901 ataatccttt tagccgcctc aaatttctct actgatacca cagggtccca cgcgccgtca
    15961 catctgatcc actgatcctg aggattcctg acacttcggg tcttcaatcg tccggaggtt
    16021 ctgttataaa cattattgcc aatatatttt tcattggtca gtatctgatg aattttacat
    16081 cggctccact ggagcccatt ctcggcgggg atgttcttcc ggttcagttc tgaagcaata
    16141 acaaactcag gcttgccctc atcaataaac atgtcataga ttttattgac tattaccacc
    16201 tcttccgcgg gccccgggat caatattact ctgtcagttt gcaggctttt cctttgcccc
    16261 ggcagaagca tgcctttcgg attagtatgt tcatcaatta ataatctccg aatgccatag
    16321 ccaggcatac caccttgata ataaccccgc cgaacgaggt ttgcctggcc aagaaaaact
    16381 ttttgtgaaa ggtttttact gtaagttgcg gcgccatagc gggagtaaga taatcccagc
    16441 ataaacaatt cggtctgttc ttccgagaaa ggctccgcgc aataaataat gcgcacacca
    16501 ttacgcttca gtagccacga gtaatgagca gcctcttcaa tatcctgaaa acgaccaaac
    16561 cgactgacat cgtaaacaag aattaacgag acttttatta ttccgttttc aacatcatat
    16621 aacagcgaac gcaggccagg cctgttatcc aggctcagcc cacttcggcc agcgtcgtca
    16681 taggtatatt taatggtaat acagtgttct tttgcatact tctcgatata ttcccgctgg
    16741 ttctccggcg aatatttctg atgctcggta gacattctta aatattgagc agcaatcctg
    16801 ttcccggcga caatatccga catagaatat tccatatgat tacgcgctat ttataactca
    16861 tcgtaaacgt cgccgtaccg tttgccgcac ctggcgtgat cgagcctgca gtctggtaat
    16921 atcgcgcctt aagctgcacg atctcgtcac catataccga ctgccagtaa aaccgttcct
    16981 gaccgtactg gacagcggca tcatccgggc ggtaatgaag ttgtatcccc acgccagacg
    17041 ccccgccgct atcaatcagc atgacgcctt gcgaactggc ggaagcatcc gcagtggcat
    17101 cgatacgtac gtttattctc gccccgacat cgcagctcaa accaatatca aagctcaccc
    17161 agtcggttga agatcccgca ccactgaatt cgcttttatc gtgatccccc atcgggacat
    17221 caacagaggg cgtggtcaca gtacaagctt ctgatttcac tgtgttcatc ccgttcaacg
    17281 tgacgttggc aaaataaaac tgattcacta ccgaagcgcg cgccagcaca ccgtccgtta
    17341 atgtaccgct ggtgatatcc ccggttttga taagctcgcc cttgatgcca tcaccgggaa
    17401 tggataccca ggtgccgcca gcgttgtagg cgtagctggt ttcatagggc aatgttttac
    17461 ctgtcgaggc cgcactcaga cgtaatccta cccctggcag gttggtgttg tagacaccat
    17521 tcccatagcc actcagcgtc ccccattgcg tcagttccca gcgggcggtc caggcctccc
    17581 gcttacaccc ggcaatgccg tcgccattgt tgtatgcccc tgtaatggca gtggcaatcg
    17641 tgctgccgac ctgtgcatcc cgctggataa ccacgttgcc gaaattcagg taacccgata
    17701 cttctgatgt cacaccaccg tagaacgaac aggtggcctg aacctccggc aggacggcta
    17761 tcatcaacag caggaagaaa agaatttttc tcatctcaac actccggcga cgtcatttat
    17821 aggtcagcgt gaaggtcgcc gtggcattgg caacacctgc agtaatcgtg gctgcggtct
    17881 ggtaataccg cgccttaagc tgcactgtct cagtaccgcc attgggagag gtatagtaat
    17941 agcgtgactg accaaactgt acggcgctgt tatccggtgc aaagaacaac tggacaccga
    18001 tgcccgtagc cgccatgtct ccgggcgcgc tgtccagctt catcacgcca gccacgttgg
    18061 acggatcctg tacggcatct atctgaacgt tgatacgggc acttttatcg cagtccagcc
    18121 cgatgttaaa cgcctgccag ccggttcccg ttcccggccc ggagaactcc gctttttgat
    18181 gctgccccag cggcacattg acatcaggcg tggtcactga gcaggcgatg gtggtgattt
    18241 gcccaccgga aaatgtaatg ggaatttcag tcgaccactg cccgttctcc cttgtccccg
    18301 cgataaaacg ccccaggttg ccgctcagcg taccgggcgt aatgtcgccg gtttttatca
    18361 gccggatccg cgcctgatcg ttaatggagg gatggtagcc aacgctgtgg cttatctgga
    18421 gcgtgaatac ctgcgactgc ccgctggcga taccgatcca gttttccggt aacggattgc
    18481 tgtcgaccga ggagcggcct tcaaacacaa atcccacacc tgcgacatta ctgttaaaca
    18541 ccgcctcacc attccaggtg acgctggcgc gggagcccgt gtttttgatg cctgcattga
    18601 tgacgtattg tggcgttgca tcataattgc agtcctgata ggccatcccc tggttcgaca
    18661 ccagccagtc gctaatgggt tcccccacca cagcatcccg ctggaccacc accgtaccgg
    18721 ggctgatgga gaaactcacc gaagacgtgc aaaacgccca cgacgcctgg cagaacagga
    18781 tccccgccag cagaatcatc ccgcgccata ctctcatttt gccacctccc tgcattcctg
    18841 gttcaccagg atcactgacg gctggttttc ctgttccggt aaacggtaat gcaccacgca
    18901 ctgttgtgct ccgttccagt taaccgcaat atcgccttcc tccggcacgc cagtcagata
    18961 gacctggcca tcatcgccga caataccgct gccgccctct ttcagcttcg cctctgcgcc
    19021 aaaggggact ggcagcccac ggtacagcag cgtcatgagc acacgccgcc cgacacgggt
    19081 gttaaaatcc gccatcacca cggcaccctg cgtgggggtc acggtctgta cggcagtgtc
    19141 ggtatccact tcgtcgccaa gcgtgctggt gtcgagcgca atacggtttt tcttgtacgg
    19201 gttcacatac ggcactaccg cataaccgcg ccagtcggtg taaaccccgg tgttgttctg
    19261 taccttcgta tcatccgcac cgggcgcttt cacgatcgcc agcgactggc cgagcggctg
    19321 cgaaagcgtg atgccatgcg gatgcgcgac aataccgccc ttcagcccgt agttaacctg
    19381 ctgcgtgtcg tcgtcatagt tgtagcctcc ggtgatttcc ccctgcccgc ctttatagtc
    19441 ggcgttgata ttgccggagc cgcccacacc atggttggtg tagctctgct ggaggctgta
    19501 gctcaggttg ttgtcggcaa gcgccgtacc gttcagccct acctgctggc gggtatcgcc
    19561 gtgtttcgcg gtgttgacgc tgtagctcgc ccaggcgttc ggcataaagc ggctgagcgg
    19621 cacctggatg ttgaacgcca gttgctggtc ggcaggctgc gtgctctccg ggtagtcgct
    19681 gtaactccag gtaacgctgt agctgatacc gtcgatattc ccgttccagc ccgcgctcaa
    19741 cgtgcgttcg tagccgtcct cctgccagta atcctgctgg tagccggaga taaaaaacga
    19801 gccatagcta ccgatactct gactgagatc taactggagg cgctggcgtt tgttgtacgt
    19861 catctgccag ccgttatcat catcagccct caggtcgttg gcttcctgga aggtgtaaaa
    19921 tccggcggtg gagtagcgat atgccgccag cgtgaagctg gtgtcggtgg tctgaaaatc
    19981 cttcgaatac tgcacccggt acgactgccc gttgtgggtt tcatcgttat tgagcgtggt
    20041 atgggcggcg gtgatatcgg ctgacagcga accgagttca ccgaggccaa aaccgagccc
    20101 tgcggcgcca gactgataat cgtccgagta cagcgtgccg ccatagacgg tgatagcatg
    20161 cggcagaccg taaatcgcag tgacctggcc gaactgcggc tcgtcgctgt cctcattgcc
    20221 gctgcggtat ttcccggcgg tcagggcata tttcaggcgg ccttcacgtt gcataatcgg
    20281 caccgccgag aacggctgga cgaaggtgcg ctcgctgccg tcggcctctt tgatggtcac
    20341 ttccaggtca ccgctgcccg aggtcggata cagatcggtg atggtaaacg cacccggcgc
    20401 gacgtagctc tggtagatga tgtagccgtt ctgcttaatg gtcacctgag cgttagactg
    20461 ggcgataccg cggatcaccg gggcgaaccc gcgcaggctg tccggcagca tgttgtcgtc
    20521 agatgccaac tgcgcgccgc gaaactggac gctgtcgaag atgtccgacg gcgtgtagct
    20581 gtcgccgagc gtcagttgtc ccttcaggcg ctggatatcc cgttgcaggt acgtgtttac
    20641 tgaatcccag tggctaccat tatcttcgtc ataattccag gtcgagtagt tgcgtagccg
    20701 ccacgcgccg aggttgatac cgctttgcag gttcgcgtag tagttatcat ccgaaccgtt
    20761 ttgtccatcg cgccaggtgt tgctgccgct gaggttgtag ttcagcagtg cggcggggac
    20821 accttcgtcc cattcggacg gatccacgta tcctctggcg ctggcattca ttgccgcctg
    20881 gggaatactg acgttcaact gcaatttact gaagcgcagt tcagtgttgg ccatcgggat
    20941 atagcggcca atatcatcca gtggttgatc cggcggcagt gctgccagtg ccgggaagac
    21001 gtccgttttg acgccccagg ctttcagcat cgcaggcgtt atctgcgggc tgagtttctt
    21061 tttggctccg tcaataaaag caatatcctg tgcgtcgctc tgcttttcac cgttcacgaa
    21121 gaccgttacg cggtaggtac caggtagctg accaccggat tccgcaaagg tggataaatc
    21181 cgccaccggc tggccggggt tatcaatctc cagcgcgtgg gggttaaaca cctcacgcgc
    21241 ctcggcatta cgcaaatagc cagacaaaag cactattgcc aacaacagcg tcagcaggat
    21301 tatcatcaga cgtggcgtcg ggttattcat cacgtcctcc ttcacagctt acggctttcg
    21361 gtctggctga caccgccata gtcgctgatg gcctgccagg tcaccgcgct tgcagtcgct
    21421 gacggtagcg tgaaactcac actgcctttc ggcggcacca tatccgcctc tttaatctcc
    21481 tgcccgtcga ccttcagggt gaacagcgtg acgtaatacg gtgtgggatt gctgacggta
    21541 agatggttac cgctgtgggc aacgctcaac tggtgatagg cactctccgg ctgcccttcc
    21601 aggcctgccg gacggtaaaa cagtttcagc cgcgacttga ccactacctg caacacgttg
    21661 gtgcgcgaat ggctgtcgtc ggtagcgggg atcgatttca cgttcagcca gaacaccgac
    21721 tccctgtcct gcggcaggtt gcctccggtg cgcacaatgc gcagcatgtt ctcttcgccg
    21781 ggattaatgc gaaacaacgg tggcgtgacg ataaacggcg ttttgccccc ctgttcgcca
    21841 ctgtctaccc agctttgcac caggtagacg ccggatttct ccgggttgcg taccgaggcg
    21901 gccgtctctt ttttatcccc gtcataaatc acgcgcgtac cgccaatcac gatcccggcc
    21961 tgcgcctgta cgctcaggaa agccagcaac agtgccagca ccacgtctgc atacctgttc
    22021 atcttcttgt tctccggacg aaaaatacgc atccctgcga aacagtccct gttatgcggc
    22081 ggtacaccag gtcgggtagg atgggagagg tggtgaaccc cgcgacggca cagcgttatt
    22141 tgtagttaat ggtgaactgc gaggtaccgt tagctgtacc tgtggttacc gcggtaccgg
    22201 tggaaacata gcgggcgacg aagtccagcg tattgctgcc tgccgcgagg gggtaatcca
    22261 ttgaggccgt atgcagagga atgactgcac catttttatc ggcgatttcg ataccaacgc
    22321 cggtcgcaat cccggtaccg ctgtccagcg ccagcaggtt ctggtttacg ccatcagacg
    22381 tcccgtcaaa cttcacgttc gccccggtaa cggtatcagg gcaaccactc aggttgagcg
    22441 tgaagcgcgt cggggaggct ttcatcccgg ccgcaccgtt cagtgcggtt tgccctacat
    22501 tgcccagatc gacgttcagg ttttgcgaac cgctgtccac ggtgcatgtc tggtcggtaa
    22561 tactgccggt aaaatggatg gtgccatcat tggcgaccgc attcccggcc accagtaaaa
    22621 aacacgcgct taacatcgtt tttttcatac gtttctcctt gatgaaagat cactcagggc
    22681 tcaaccctga ttcactctgg tgacgagaaa acccgcgtaa gactttcccg ttagtgccag
    22741 agtggtgagt tcaggagaaa atgtggtgga cgaagggggt gaaaaataaa atgtcagtgg
    22801 aagtgaagta tggctttcaa aatggcataa aaccgtaatg cggtgagtga gtaaaaactg
    22861 gcaacgtgaa ccacttaagc acgttgccag ccaggaccag agagagacta catatttgtt
    22921 acattaaata ttattttaaa aacaacaaga tagtccgtta ccaaaagggg tctcagaaaa
    22981 attttgacag tttgctaaat ttttctgata ctactttaca ttctggcact aattgtgtgg
    23041 ttttctctca cgtttcccca gacattcata aacctctgcg tatttgtcgc cgtataaatc
    23101 accgtatgcc ggatgttccg gtgccccaga taatcctgta tcagacgggt gtcgcgcccc
    23161 agatctgcca gcgcgtaacc gcaaccgtgt ctcagcatat gcggataagc ggaaatgctg
    23221 acacctgcca gttctccgta acgtttgagt aacaatctaa cctgatggcg cgacagcgca
    23281 ccacccttct gcgacaggaa caaccagtct gaatccgcat cccgccagcg gttgcgtacc
    23341 gccagccagc gcatcagcgc aggcagttca ttatcaaaca gcggatgctg cgtggagaga
    23401 ctgtttttca accgccggac atacaccgag cgcccgatca gatcgatatc agacaggcgc
    23461 atcccgcaca gttcactgac ccgcaggccg tgtaaaaaac acatctgaat gaggcaataa
    23521 tcgcgctcaa aatggatccc tctgtctgct gcaagcaata tctgtcccac ttcatgacga
    23581 gtgagatgct ttcgtttttc aggcatcagc cttgctcctt atcaaaataa aaaatgaaaa
    23641 aaaatcaggc cagaactgcc ttcacagtcc aggcctgaac cagagaatac caccagaaac
    23701 aacgtctggt gatgatgtaa aactcaacaa ttttctgtaa cggcttccag tttcataccg
    23761 ttgatgaaca gatattgcgg tacacccgac agaagaaata tctgctgtcg gaggcgcgat
    23821 gcgagcccaa tacatccttc tgctgctgtg cgctgctttc cccctcaggg cgattactac
    23881 gccggagatc gcagcctctg cgcttacgcc ggactgtgtg aaataccggg tggtcggcct
    23941 gtgctactgg ctgtactgca cgccgttcgg ctgtacggta cgcacgtcgg tgaaggtcag
    24001 ccatttccgc ccggatctgg tggtgtcggc ttacagcaac accgggcaga atccgtgggc
    24061 ggagatgtcg ttactcagcc caccgttgcc cggtgttgcc gaaggcggcg gcgatacgaa
    24121 tcctcgttct gccagccagc acagcaaggt ccgctttaag aatgccgatg ccatcggctt
    24181 cccggcgggt gatgaactgg caaaattttt cacccagttt ggctatatct gctcaccttc
    24241 atcgcagcct tttcaaccgt actttctgag tcaacccgat acgctggcgt ggcgcagcgg
    24301 tgtgccggaa atgacatacc cggaggcgct gacgccgggc atgcgggaag tcggtcagaa
    24361 cggcgatatg tggggcaata tctacccgcg agccggagcc atcaaccaga cgcacgacta
    24421 taaagccgcc gccgttatcg cccagcgcgc ggccgatctg gtgacccgta ccgggcaacc
    24481 gcatctctac acgccgctga ccgcctcatc gcgcgccggg tactggccgc cctccccggt
    24541 tatcgaaggc gacagcgata accatcgctg gcaaatgctc acgccgaaaa aatccgccgc
    24601 ctgttcggtg ttcccggatg gcagtgccac tgacacctac gccaacaaac tggcggagga
    24661 tggcgcgtac gcctggacgc tgtggcggcc ctataaatgc tgtccgcgcc gggggcaaac
    24721 ctttctcggc agtacgggtt aacggaggcg gtgatgagag cccttttcct ggtcttattg
    24781 tttttcgccg gggtttgcgt ggcggcagac gactacagtt tccgcacgcg aggcgcggtt
    24841 gacgacctgc tttactacca gattggcggc ggtgaagcgg tcatgccgcc tccggtacgc
    24901 cgtagcccct cgccgcttag cttaggtctt ggctggaacg ccgatatgat gtgcggcaat
    24961 ttcgacatcg acaccaccgt gcgcaaccag ctcaacggca tcaccgacgg cttccagcaa
    25021 ctgatgggca acgttatcca gagtgccacc ggggcggtcg ccagcctgcc cgcgatgatt
    25081 atccagcgcg ccaatccgca gctttatgat ctgctgacca acggcgtgct gcaggggcgg
    25141 ctggattttg ataagtctct gctgaactgc cagaaaatgg cggagaagat gaccgatttg
    25201 gcggccgatt ccgcctggtt ttccggagcc aaagcggaga actaccagtc cctcgccgcc
    25261 agcgataacg atgccatccg caccgaccag aaagcggcaa aagaggccgc cgaaaagggt
    25321 aaacgctggg tgggcggtga aaagcgcggc ggcaaaggcc agctgccgat taaggtagtc
    25381 catgacgcca ccgccgcagg atggaatatc ctcaaccagc agcctgccac cagcactact
    25441 tctcttagct cctctgaatg cgacggggaa ctttgtagca cctggacatc ccctgaagag
    25501 gccgccgggt ggatgacccg cgtcgttggc gagcagacca tcagcgtggc gcaggtcacc
    25561 gacgatccgg atgcgcgttc cggcgcgcag gcggggatcg gcctgcatcc gctgatcgac
    25621 gaagaaaagg cgaagatcct gcccctcctg acagatctgg ttaatggcag tcagaaaccc
    25681 ggcttcgaca atttggcgaa agcttccggc ggttctatgc aactgacccg cggcgtggtc
    25741 gaggcgctgc gcgacgatcc ggacgttgcc gtcctcaccg aacggctggc gggagaactg
    25801 gcgctggccc gcgtcaccga gcaggcgttg ctggcccggc gtacgctgct cgcaggtatg
    25861 cgtgaaccca atatcgccaa cgtcaaagag gcgcaggaga gcctgggcaa aaccaccgcc
    25921 caactggatg aagagctgaa ccagttgaag ctggaactcg atttccgcca ggcactgacg
    25981 cgcaataccg ccagccagat cctgcaacgc aaacagcagc gcgaccagct tcagggacag
    26041 gtggtggagg tgccggacga cagcgacagc cgtctgcaca acctcaataa cccgcagccg
    26101 gacagcccgc gatgaccgcc aacagctatt tcgagtacgt gctcaccctg ctcggctggc
    26161 tggtgaataa cggcgtgtgg cagacgctga ccgccaccgg gctgttcgcc gtgcccctgc
    26221 tggcaaaact ggttgccctg tggctgaaag cgcgcggcca gggggccgat gaaggcaacg
    26281 tcgcccgcct gctggtcgtc tgggtggagc actcgctcta cagcgcactg ctggtgatct
    26341 tcttcgcggg tgtgccgttc ctcaatgtcg atatcaacac cctcacctac gacagccagc
    26401 gcacaaagca gtgcggctgg tcggtggtac agcccggcga ttccggctac gcacctttga
    26461 ttaatgaact cagcggccag agcgcttccg ttccagtgtg gtggtacttc acgcatgcac
    26521 tgggcaaagg catcaccagc gccgccgtcg ccgtattgcc ctgcaagccg gacctgcgcc
    26581 agttgcgctt tgaggtgcag cacacccggc tcgccgatcc ggcactggcg caggagctac
    26641 aggatttcgt taacgactgc tacgggccgt cgcgggcaag gctcaagcgg ctgagtagcg
    26701 atattagcga ggagcaaagc cgggaagtgg actgggtggg atcgtctttt tacctcgcca
    26761 cgctaggcta ttacgaccgc gatcactccc gttcgccccg cgccgcctgg ccttatgacg
    26821 ccagccgcga cgacggtctg agcgatacgg gtggcggtgg ctatcctgcg tgtaaggagt
    26881 ggtggtccga tggcgatacg gggctgaaaa accgcctgct gaagcaggtc gataccacgc
    26941 tctggcagca actgcaaaaa ctgggacagt ctaaagagga gtatgaagaa gcggtactgc
    27001 gctcgctggt cagcccgcgc aatatgcagg tatcgcaggg cggactggtg tacaacgggt
    27061 ttggtgggag tgcgggggat aacctcagcc agacgcagtt gctgagtcgt gtgggcggga
    27121 tcatcggcgc ggggctttcg agctacgccc tgtttccggc gtttgataac gtccgccagg
    27181 cgctgccgat ggtgcaggcg ttcctcgaaa tggcgctggt gatttgtatt ccagtaattc
    27241 tgctgttctc ggcatgggat cttaaaacgg tcatcacgct gagctttgtg cagttcgccc
    27301 tgttctttct caccttctgg tgggaactgg cccgctggct ggataactgg ctgatgcaga
    27361 tgatgtacga cagcgatacc cactcatact tcaacctctg gggcctgcaa aacacctccg
    27421 acgatctgat tgtaaatatc attatgggcg tgatgttcct ggtgttaccc gcattctggc
    27481 tgggggcgct gacgtgggcg ggcatcagga tcggcgcagc agtcgctggg gggatgggca
    27541 gcgcggtcgg cgatatccgc agcgctggcg aacaggtcgg gaagatgatc gtcagtaaaa
    27601 cacgcattcc ctgaagattt cgtactgacg gtgtgatgaa cagggcttgt agctgagttt
    27661 atgcgcaccg cataaactgt cgtgtcagtg acaatctcgt ggtaggtagc tggagtctga
    27721 aaaaacgcaa cgggccgtta agcccgttgt tcgttacatc accgcagttc tatcactctc
    27781 gcccgctcag cctgctttcg gtagtggtcg tatcgctccc ggaaccatgc ttgctgttca
    27841 tcactcatat tgccggtaac ggcatcgata tcaactggct ggcccagggc gtgcatacgg
    27901 gcaaaactgc gcgccagaaa atcaaaattc tttaatgcgt ttatatcttg tcggttcatt
    27961 agcatatccc tccagtcatt gtgtttactg ttcaaggtca tatgcttttc tgatttcagt
    28021 atccgcctga aaaagatcgt tttttgagcg atgcgtgcgc atcaatctac ctgtgttcac
    28081 atttcaggaa cgacaaaact cttcagcccc agtaatgatt ttcccctccc ttttatcacc
    28141 cgcgcagaaa acactaccgc cagccatgca gtaagttcgc catgtatccg gaaggtgcga
    28201 acggcgcaga tgcgcaggat acccgtacca ttccatcccg cagggaaacg ccctgcgaag
    28261 ggtgttccct gctcatttac aggagaaaca ccatgtcgct tttcgataaa aaaccgtcgt
    28321 taatgccggt ggttatcgcc cctgatgtct ggcaatccgc agtggaattt cccgccgacc
    28381 cgcagcaggt ggaagaacgg ctgggcaatc tgttgctcgt cgtactgctg accctgcgca
    28441 ccgccgggac atcccgcaag aaagtcacct ttacgatcta ctgccagccg ccgcagggcg
    28501 atatgaaagt ccctgtcgca ttaccgctat cgctcaacta tcacggccac tatgtgcagg
    28561 tctcgatggc tgaacaggca taacctctcc ccgccggagt cgcttccgga tggaggaggc
    28621 tccggcttct ttccatgagg accacacgat ggacagaagc aacgatatct accagaaagt
    28681 caccgaccag attatcgctg agctggaaaa cggcaatgtg ccgtggctcc gcccgtggcg
    28741 cgatggcgag ccgccgttcc cgatcaatgc gttatccggt cgtccgtatc acggcatcaa
    28801 tgtaccgctg ctgtggaaca gcgccgacag gcagagcttc gccagcgacc gctggctgac
    28861 gttccatcag gtcagcgcgc tcggcgggca ggtgcgccgg ggggagaaat cctctctggc
    28921 ggtgttgtat ctgccgcgca cgcaggaaga gaccgacgca ctcggccagc cgttgctgga
    28981 taaagacggc gagccgaagg tgcggcattt cgggattatt cgtgaatttt ggctgtttaa
    29041 cctgtcgcag tgcgacggcc tgccgcaggc gctgtccgag ccgtttaccc ggcccgcaac
    29101 gcctgtggag atggctgaag ccattacgat gaatagcggt gtgacgctgc gccatcgccg
    29161 ccagtcgaaa gcgtattaca aacccgcgac cgatctggtg atgatgccgc acccgcaaca
    29221 gtttgaaagc agcgaggcgt actacgccac gctgctgcat gaactgacgc acgctaccgg
    29281 acacgcttca cgccttaacc gccccggtat tgccggagcg gaacgcaccg aagcactcta
    29341 cgctgctgaa gagttagtgg aggagatggg tagcgccttc ctgtgcgccc attgcggcat
    29401 tcagggcagg ctccagcacg ccagctatat cggctactgg ctggatatcc tgcgccagga
    29461 caagcgggcg gtgatccgcg ccagcggtat ggcgcgccac gccagtgaat ggctgctcaa
    29521 cgctgtacca caaccctgtc agcttagcgc ctgacagccc acccgcacgg ggagccttcc
    29581 ccgccgggaa gttgctcccc tttttctgag gatcaatgta tgggactcga tctcttcttt
    29641 ttcgaacgga aaaaaaacgc gcccgatgaa agccagtacg ccagagtggg ttatttccgc
    29701 aaggtgaatc cgctgcttaa atgggtggat aaacacgttg ggctggtgcg caaccaagtg
    29761 ctgttagaag tcaccaaagc aaatttgcag gcgcttctga gcgacctgca gagcctgacg
    29821 ccggataact gcggcgcggt ttatccgacg attgacggct ttttctatgg ttataccgat
    29881 tatggagaac actactggaa agacgttcgc accgtcactc tctgggtgaa ggagacgctg
    29941 gagacgtttg attttgagcg agacaggctg atgttttatg cctggtggta gtattaaagt
    30001 ggccgacaaa acgtcggtca ctttgcctcg tcggccagaa atacagcgcc agatatacac
    30061 atgagagcat accgttttta ctaataaaaa tgtaaaaggc ccaaagggag acactgcaaa
    30121 ggaagggcga caagggcaaa ggggcctgcc cggaaagggg aaatatgctg aagtggctaa
    30181 aaagaccatc ccagagccag ccacctacgc cagacgctac gctgccggag ggctggtttg
    30241 cgccacaatc cgcagaacaa ttgctggcga cgccgttacg ccagtcctgt ttgcaccagt
    30301 tacggcaact gaccccgctg ccggataccc tgtttgatag ctggatcctc gcggcgattc
    30361 atcgcttcgc cgcgttggta caactgcttc ccgcctcaca aagtcatcac cattccactc
    30421 ccggcgggtt gctggatcat tcgctggagg ttgcctgcaa cgccatccga ctgcgccagg
    30481 gttatctgct accaccggaa gcgggcgcgg aagaacaggc ccggcaggcc agcgcatgga
    30541 cggtcgcagt ggtctgcgtg gcattactgc acgatacggg aaaaatcgcc gtggatatgg
    30601 agattcatca acgtgatggc acccgctggc attcgtggca gggttatctg gcacagcctt
    30661 atcgctggca gtaccagagc cacggtcgcg actatcatct gcatccggcg gctaccgcaa
    30721 tgctgattaa tcagttgata ccctcgccat tactggactg gctggccgga tacccggcgg
    30781 cttttgcgtc cctgatttac cagctcaccg gacactacga acggtctggc gcactggctg
    30841 aactggtgca gcaggccgat aaagcctctg tcgcccgata cctcggcggc gatatcaaaa
    30901 ccgcgctggc gcaaaagccc tcttccttcc cccagcaact tcttacagcc ttgcggtcgc
    30961 tggtacagaa cgagtaccgg ctcaataatc ccgaatgcgg atccgatggc tggctgacgg
    31021 aggatgcgct gtggcttatc agcaaaacca ccgccgaccg ggttcgcgcc tggctgctcc
    31081 agcatggagt ttccagcgtg ccggaaaaca ataaccgcct gtttgacgaa ctgctgacgc
    31141 atcagcttat tgttgccaac gaagataaag cgatttggcg ctgcattatc cgatcggata
    31201 aaggctggtc gcccgttgaa ccgctgacgc tactgcgcct gacgccgtct gtaatctggg
    31261 ggcaggatga tcaacgtccg gcctgtttcg atggtgatgt aacgccggta attgaagccg
    31321 aacccgctcc tgaacttagc gcaccgactc aaccgttacc ggagatagca gacgatccga
    31381 ggcttggtga caataacaat ttcctgcagt ggctgaagaa ccacactagt ccttcagcca
    31441 cggtgaatac ccggaacggc aaggtacatg tggtggaaaa ccatcttttc ttgatttcgc
    31501 cggggatctt taaaacatac gcagcagaaa ccacaggcga tagcggcgac ggctggaaac
    31561 tggcgcaacg aatgtttcag gagtcagggc tggccctgcg ctgtaatgat gacagtttta
    31621 tctggatctg cgaggttcgg gttccacaaa agaaaagtag tcagttgaaa gggtttctga
    31681 tggaagatcc gaagcaggtg ttgggggaga aagtgccggt gaataatccg tggctgaagt
    31741 tgctgcgcga atagctattt atatcaaaat actctcttcc accgagctac agcgaatggg
    31801 ctttaatacc taaagagccg catgatattc aataattgat ttcaattctt caggcgaaaa
    31861 aggcggatta cgccgatgca acatggcgtg gcaatttggg cagacgggaa taaaatcttt
    31921 tttgggatcc agagagtaat tctctttgat gtctgatagc ggaatcaggt ggtgtatatg
    31981 gataaaacct ttcgctatct cgccatacac cttcatcata tcaataccac atactttgca
    32041 cagacaacca tgccaggcta atgcggcctg tcgggcctta cggtcgcgtt catagcgatt
    32101 aaccgttacc tgcgtagctg caccttcagt aaatacgctc tgcggtggca gttcatcagg
    32161 aaaaatactg atggcgggct gctcgttacc caaatgtttc tcaacgatct ggaacccttg
    32221 atcggtaagc aacttgacgc atttcgactt gataccacca ctaaaatctt caggggaaaa
    32281 ctcaacgcca gtcatatttg aagcagcaat accgactata gctttcggtg gataactgcg
    32341 cccttcaaag accacatcat acagccggga ctcgctgaac tggtggcgta caccttcatc
    32401 ataggcacgc atagccgcaa taatgcattc cctggaaagc tggtctggca gtttcttctt
    32461 catttacaac tcatcatccc tgaaacgaca tttgctaact ctttatttta tagcatatta
    32521 atttatccgg tgataaacag cactcttcaa tttcgcgtta ccgggatagg ggcatcagtg
    32581 cagcggcaac gtaacgtagc ggcagagaaa tcgcgcctca ttctggttgt ccagacgtac
    32641 ctgaaagata tcgtgtgtat cctgcgcgga tgcatcctca cgccacgcca gcagaccccg
    32701 ttggttcatg cggataataa tctgatcggg cgtttcgccc tgattcaatg cttcgactac
    32761 ctgttgtagg ggaagcggta gcgtctgagc aggccacatc acttcaacac ttacgagctg
    32821 gcggtgtgtc cgaggtgaca tcggtggttt cctccatggc tggaatttag cggttaatta
    32881 gccatgaaat aggggcattt tttactgttt caactactgc cgagatggct acggaatacg
    32941 cgctgactga tgcaaaaata gcttctctta tgtgctctgg aaatccagat ggtacgctag
    33001 tgaaaatggt ggttccgtcc ggatagcgcc atggcctttt tgttcatatt tccacagatc
    33061 accactcctg ctcccagcca tcagaagagt ttttttaccc ctccagctta cgacctgaag
    33121 cttaaggtcc agagcggcaa gcagcttaaa gagcgtatcc aggctggtcc cctcaggatg
    33181 gttctcaaac tctgaaaccg ttgactgttt catgcccata cttgctgcag tttcacgctg
    33241 ggtcaggtta cgttgcttgc gtgcatccct gattgcctga gacaacattt tgggtgaggt
    33301 tacgttcatt ttatccccca ggatagatat tttgagcata tcccctatag gggataaaaa
    33361 ttcatattct cagcggagaa aagattaata actaaatgaa ttaaaattac ttttcatgga
    33421 aattaagggg ggacgatatt cctcgagcca gtaaatatga acattgccag atggcagaac
    33481 gcgccatttt acaagcattt tccataacag atataagact gattgagcgg ttggattaag
    33541 catgacgata tgtttaaaaa aaccagtttt atcgacataa cgccaaaata tgtttacagc
    33601 atcgacatat caacgccata tgtacatttt ttggcatttt ttaaacatat catcagcagg
    33661 ctttattttt tgctatcgaa atctacagtc cctcgtttca atacaaaaat tccagcgtgt
    33721 cagctacctt ttctgggttt ctttattggt tttagcagtg cagcaagaga tgcagatgtc
    33781 acgacttctg ttttaacttc agaatcgatt gatttttctt gtgatgtcga gaattgcttg
    33841 tcgaaggcat cgttttttat cacgtttccg gtatgcctgt ttgtgtgaga gatagcagca
    33901 ggacgagaaa cacgcgtttt atttattagc cgggaatcct gaagttggcg tccctgttca
    33961 tcctcagcgg caagtttgtt cagatccttt tcaagaccca aaatctgcat aactgacaaa
    34021 taagcgccga ttgtcacgcc cgtcccgcct gattcaagag aacgcaaggt cattggcgac
    34081 atcccggctc gctcagcaac ccgcttcgct gtgagcttac gtcgcagtcg cgccagctta
    34141 agccgttcgc caaactcagc aagcaactga ttagtaccgg gaactaaggg agccgttttt
    34201 ttagccatag tgataatatt ttatcttttc aatacctata tagccataat attagcgcat
    34261 catgccgaag gtgcggtgcg ccgtggaagg tggaacatca agtttacctg cgttctccac
    34321 tatctttgat gggaaccagc aagcctttga ccttcagttt gatgatgtcg cgactgactc
    34381 tgggctggca ttattgataa cataagcgca tcttaatcgg gcagttggcg ttacgccctg
    34441 ccattgttca caataatacc gttcatgatt catgaacaaa actctgttgt gaacatttca
    34501 tctaaaacta catcctcaac catcacttgc cataccatcc cagaaatcat tgatgatctg
    34561 ctcaatccca gcatcaccca cgtcatgttg tcgtactatc acatcgatca ggcgttcttt
    34621 atcaagatgg agtaaatcct gcaaggatga gtgttcacta attgtcgcta tatcgcttcc
    34681 caacagaaaa gcgcccatac gtatgggatc ccagttcagg cgttcccctt cgacgggcga
    34741 ttcatagatg cgttcattct cgataacacc atattcacag ataatcttaa agagatcggt
    34801 cgcgtcttta gtactcccat gcccacgatc acgccatgca aaaagtttta acagagccag
    34861 tcctggcaag gagcagaacg gaatattttc gctgttatcc agccgcacaa ctacggtatg
    34921 agaaaagact tcggcaaatc catccacagc cattatgatc tcacgttctg gtggccaggc
    34981 aatttgattt ccttcagcaa caccaccaaa aggaataata tctagctcga tccctttgac
    35041 aatcatacga tgggcattac cttggacaac ttctgcccct tcagcaagga aagcattttt
    35101 aagcgcatca aatctcggcc agctatccac aaagacggca atatcaatat cacgggttcg
    35161 gcgaccaata cttctgctgt gtatatgatg gagcagaatt tcacgagccg ttgcacctgc
    35221 aataaaaaac gggatcccct gagcggtgca aatacggttg atctgactca gtaacgtttc
    35281 agtagctgaa gagatcggat tcattaaggt gaagatattt gtcattgatt atccttgctg
    35341 tttcccagtt tctgtcatcc ccgcttgcca gcagttcagc cacgcatagc atcgcctcgg
    35401 ctttgtgatt cagtacaaag ttcccccaaa aaccggcgat cagctgtagt ttcccccccg
    35461 gaacaggttt tagtcccagt tcctttcgcc tttgtaacaa cggatgggat gtaaaaatca
    35521 cgccattttc tggtatcagg tatcctccgc tcagctttgc cgccgcaatc tcgccgctcc
    35581 agtattcccc ggacgcaagc gtcaactcat cccatgaaac aggcgcggat aaagaaagcg
    35641 aatccagctt agggcgcaaa gctgtagcat aatctttcag ccacaaagcc tctaactcgc
    35701 cttcattgat taagcgacgc cccttcttcg atttgcggaa atagcgttgc gcttcgagat
    35761 aatcaaaagc cttgctgacc attcccagag agatacctgc ctttccggcc agactgcggt
    35821 aagtctcgtt cagggtctct gggctagaca gcaaaacaaa aagaagcttc atcacccctt
    35881 cagcaaaacg actgctggcg gaaacgggtt tcttttcata gcggccttcg atccgcacat
    35941 acaagcctgg aacctggata cgggcattcc ctgccgtatc gataaagttg atctggctgt
    36001 cggcgcaata ttccgccagt gcagacgtca ggcggttaca caccagcagc acaggaaaat
    36061 ccgcaggaaa gcgaaccatt tgttcgcgaa cagccatcag tgactcttta cggtggataa
    36121 attttacttc cagagcaaat atcactgact caccatccgg ccctgttagc ttaccccagg
    36181 catcgtgctt accatgtcta ccgctatgtt catactgcaa atggaatcct tttggcagat
    36241 tctctatcgc atcagacaaa agctgttctt ccttcatttg ttcacacctc ataactgttc
    36301 atgaatcatg aacaaaacaa tgttatgaac aaataaagaa agaagcaacg taaatgatca
    36361 aaaaaagacc gccagaaatg cgatgcctta ttcaaccttc caaacgtcat tttccattaa
    36421 agaaagaagg gtaaagagta gttggacgca ttctttcagg gttgagtaac cattcctact
    36481 cccccgactc cacctcctcc tgcgccgcca tcagaaccaa ctctgacttc gaaatcagca
    36541 ggtctttcgg cctcccacct ctcaacctgc gattagccga gccaaaccag atagccagcc
    36601 cccacggtgt tttacgctcc ttaaacagtg aaagaatcaa acggacaacc ttgagaggcc
    36661 gcattttctc atccagaaca taatcagggt ataaatcctc accatcgacc ttcagcgaga
    36721 atatcttccc ggcggctttc cagcctttcg gaccagcatc aggatcggcg gaacgaaagc
    36781 ccgcttttgc ggataattca gacgccgtca gccattccga gtcagccagt attctggctt
    36841 tcagctcctc tagctgccgg tcattgcgct cctggaaact catatggtcg agtgcgcctc
    36901 ccggcgactt tttgaagcca tacggcacaa catgcctgaa ccggtttgca tccagataat
    36961 ccgcccacca ctgcatcatc tcccaacgtt cttcgagatg ctgcgccttg tggatatacg
    37021 ccgcacgcac gcgcttgcgc tcctgatggc tcatctggcg ctctaccgcg tcgcttgacc
    37081 acagccccga ctccaccagc gcgctacagg ccatagtgcg aaaaccgtgg ccacagacat
    37141 ctttctgggt gtcgtagccc atcacccgca gcgctttgtt gatggtgttt tcactcatcg
    37201 gcttgcggta attgtgatcg ccggggaaca ccagttcagt tcccggtcgg caatgctgcc
    37261 gcacctcgtg cagcagttcg atggcctgac gtgacaatgg gaccaggtgt ggggagtgca
    37321 tcttcgcccc acgcgctgaa tatttaacgc cggggatcgg ctcacgctca gcggggatgg
    37381 tccacatggc gttacgcaga tcaatttcat cccagcgggc aaagcgcagt tcgctggagc
    37441 ggataaacag cagcagtgcc agttttaccg ccagttgagt cagttgacgg cctttgtaat
    37501 cgtcgattct ttccaggaaa tcagggataa gattcagatc caacgccggg tgatgccgta
    37561 ctttgggcgt ggagaccgcg ccggttaaat ctgatgcagg gttatgatcg ataatgccgt
    37621 tctggacggc gtagcgcatc acgcaggcag tgcgctgctg aagccgggaa gcaacatcca
    37681 gtttgcccgc tttctccact tccttgatgg gcaccagcag atctttgacc ttcattttgg
    37741 tgatgtcaca gctgccgttc gtggggaata cgtaaagctc gaaataacgc agcacccgct
    37801 tcttatgttc cgccgaccag ttcacgttac tggtgaccca ttcacgtgcc acagactcaa
    37861 acgtatgttc cggcgttatc gcaaggcgtt ctgcttttcg gcgtgcactc ggatcgatgc
    37921 cggaaatcag caataattgt gcctcttcac gcatgcgtct tgcctgtgca agcgtcaaaa
    37981 gcggataagg gcctaaagcg agctttttct ctttgtttac aaagcggtac ttgatgtacc
    38041 agcgttttga accgttaggg ttaaccagca ggtaaagtcc atgcgagtca ctgagcttat
    38101 acggcttgcc acaaggcttg gcgtggacta tagcgcgatc tgttagtgcc atttgggggc
    38161 ctcactatta tcgaactacg tctaagcccc caatttggcc cccaaatcgc gtggatgtca
    38221 atggatgaaa aatgaccaac acggaaagac cacaaaataa atatggttat tgtggaaaaa
    38281 taaattacct ttgaattaca aacggatatg gacgaatttg gacggaaatg gacacaaaaa
    38341 aagccaccct taggtagcta attattgcat cacttggtgc cgaaggccgg actcgaaccg
    38401 gcacgtattt ctacggttga ttttgaatca actgcgtcta ccgatttcgc cacttcggca
    38461 ctgaagaggt atgcggaaaa cgttgtggat tatacctgtc gcacgccacc atgcaagcgc
    38521 cagcttgctc gcacctcgct aagtgctgaa attttcagca ttgcataccg cgatgtgtaa
    38581 cctttgtcac actccaggcg ccccgccctg ccatgctcta cacttcccag accacaccag
    38641 agaaggataa aaacatgtcg atgataaaaa gttatgccgc aaaagaggct ggcggcgaac
    38701 tggaagttta tgagtacgat cccggtgaac tgaagccaca agatgttgaa gttcaggtgg
    38761 attactgcgg gatctgccat tccgatctgt cgatgatcga caacgaatgg ggattttcac
    38821 agtatccgct ggttgccggg catgaggtga ttgggcgcgt ggttgcattg ggtagcgccg
    38881 cgcaggataa aggtttgcag gtcggtcagc gtgtcgggat tggctggacg gcgcgtagct
    38941 gtggtcactg cgacgcctgt attagcggta atcagatcaa ctgcgagcaa ggtgcggtgc
    39001 cgacgatttt gaatcgcggc ggttttgccg agaagttgcg tgcggactgg caatgggtaa
    39061 tcccactgcc agaaaatatc gacattgaat ctgctgggcc gctgttgtgc ggcggtatca
    39121 cggtctttaa accactgttg atgcaccata tcactgccac cagccgcgtc ggcgtgattg
    39181 gtattggcgg gctggggcat atcgctataa aacttctgca cgcaatggga tgcgaggtga
    39241 cagcctttag ttctaatccg gcgaaagagc aggaagtact ggcgatgggt gccgataaag
    39301 tggtgaatag ccgcgatccg caggcactca aagcactggc ggggcagttt gatctcatta
    39361 tcaacaccgt caacgtcagc ctcgactggc agccttattt tgaggcgctg acgtatggcg
    39421 gtaatttcca tacggtcggt gcggttctca cgccgctgtc tgttccggcc tttacgttaa
    39481 ttgcgggcga ccgcagcgtc tctggttctg ctaccggcac gccttatgag ctgcgtaagc
    39541 tgatgcgctt tgccgcccgc agcaaggttg cgccgactac cgaactgttc ccgatgtcga
    39601 aaattaacga cgctatccag catgtacgcg atggtaaagc gcgctaccgc gtggttctga
    39661 aagccgactt ctgatccctt tgccgggtgc ctgtcgcccg gcgtttaaat ctctttggaa
    39721 ggcgtctcgc aatctgctga atttagcggt gaagagtgcg cagccgttgc ctatactcca
    39781 ggcaggaaac tggaggatat ctcatgagcg aacccctgtt aattgcccgc acgtcggaca
    39841 cagaactgtt tttattgccg ggaatggcga atcgtcacgg gctgattact ggcgcaacgg
    39901 ggacgggtaa aaccgttacg ctgcaaaaac tggcagagtc tctatctgaa atcggcgtgc
    39961 cggtgtttat ggccgatgtg aaaggtgatt taacgggagt agcggaggaa ggtacttctt
    40021 cggaaaaact gctcgcaagg cttaaaaata tcggcgtaaa tgactggcaa cctcatgcta
    40081 atccggtggt ggtgtgggat atctttggcg agaaaggcca cccggtgcgg gcgacggttt
    40141 cggatctggg gccgctgttg ctggcgcgac tgttgaatct caacgatgtg caatctggcg
    40201 tgctgaatat tatcttccgc attgcggacg atcagggatt gttgctgctc gactttaaag
    40261 atttgcgggc gattacccag tacatcggcg ataacgccaa atccttccag aatcagtacg
    40321 gaaatatcag tagcgcatcg gttggtgcca tccagcgcgg attactgtcg ctggaacagc
    40381 aaggcgcagc acacttcttt ggtgagccga tgctggatat caaagactgg atgcgcaccg
    40441 atgccaacgg taaaggcgtt atcaatatcc tcagcgccga gaaactttat cagatgccga
    40501 aactgtacgc cgccagcctg ccgtggatgc tttcagagtt atatgaacaa ttgcctgaag
    40561 cgggcgatct ggagaaacca aaactggtgt ttttcttcga cgaagcacat ctgctgttta
    40621 acgatgcgcc tcaggtactg ctggataaga ttgagcaggt gataaggctt attcgctcaa
    40681 aaggcgtggg cgtctggttc gtttcgcaaa acccgtctga tattccggat aatgtgctcg
    40741 ggcagctcgg taatcgtgtt caacacgctt tgcgggcttt tacgcccaaa gatcagaaag
    40801 cagtaaaagc tgcggcgcaa accatgcggg ccaatccggc atttgatacc gaaaaggcaa
    40861 tccaggaact ggggaccggc gaggcgttaa tctcgtttct cgatgcaaaa ggaagtcctt
    40921 ctgtggtgga acgggcgatg gtgatcgcac cttgttcgcg aatggggccg gtgacggaag
    40981 atgagcgtaa tggcctgatt aatcactccc cggtgtatgg caaatatgaa gatgaggtgg
    41041 accgcgagtc cgcctatgag atgctgcaaa aaggctttca ggccagtacc gagcagcaaa
    41101 ataatccccc cgtgaaaggg aaagaggtgg cggtggatga cggtattctt ggtggattga
    41161 aggatatttt gtttggcact accggaccac gcggcgggaa gaaagatggt gtggtgcaaa
    41221 caatggcgaa aagcgccgct cgccaggtga cgaatcagat tgtacgcggg atgttgggga
    41281 gtttgctggg ggggagaaaa agataagtca gacgtgatct gtgcccctca ccctaaccct
    41341 ctccccaaag gggcgagggg accgttcgaa ctcgtatttg actttgtcaa caataataaa
    41401 gggagctttc gctcccttgt tagattacga ttttctcatt aacagccaca ggctgattaa
    41461 gaagaagctg gcgcttggca acagtgcgcc gatgatcggc gggatgccat aaaccaacgt
    41521 cagcgggccg aagatctggt ccagtacgta gaagacaaaa ccgaagctga taccggtgac
    41581 cacacgcacg cccatcggta cgctacgcag tgggccaaag atgaacgaca gcgccatcag
    41641 catcatcacc gccacggata gcggctggaa gattttgctc cacatgttga gctgataacg
    41701 tccggcatcc tgaccgctcg acttcagata cttcacgtag ttgtgcaaac cactgatgga
    41761 gagtgcatcc gggtccagcg ccaccacgcc cagtttgtct ggcgtgaggt tggttttcca
    41821 ggtgccgctc accgtctgcg aaccggtaat ctgtttcgga ttggtcaggt cagattcatc
    41881 aacctgcgac agacgccaga ctttatgttc cgggtcaaac ttcgcggtag cggcataacg
    41941 tacggattgc agacgacgat tctcgttaaa ggcataaata ctgatgccac ctaactcctc
    42001 gtcaccttta acccgctcaa tgtagacgaa gttgttgcca tctttcgccc ataagccttg
    42061 ctgggtagag agcaacgagc cgccgtacat cgcctgcgca cggtagttac gcgccatctg
    42121 ctcgccctgc ggcgcgaccc attcgccaat cgccatcgtc agcaagacca gtggaatggc
    42181 ggttttcatc accgacagcg ctacctgcat acgggtaaaa ccagaagcct gcatcaccac
    42241 cagttcgctg cgctgcgcca gcatcccaag accaagcaac gccccaagca gagccgccat
    42301 cgggaagaag atttgcacat ctttcggcac gctcagcaag gtatacattc ctgcgcctaa
    42361 cgcgtcgtaa ctcccctgcc cggctttttt cagctgatcg acaaacttga taatgcccga
    42421 cagcgacacc agcatgaaca gcgtcatcat gatggtggtg aaaatagttt taccgatata
    42481 gcggtcaagt acgccaaaag gttgcatcac accgctcctt tacgcgaaaa actggcgcgc
    42541 aggcggcgga ccggcacggt gtcccaaagg ttgagaacaa tcgccaaagc cagataaatc
    42601 aggttaacgg tccacatcca cagcgtcggg tccagcttac ctttaccgcc gttcgatttc
    42661 agggaggtct ggatcaggaa gaaaagtaga tacagcagca tggctggcag catcgacagt
    42721 acgcgtccct gacgcgggtt aaccacgctc agcggtacga ccataagtgc catcataaac
    42781 acggtgaata ccaacgtgat acgccagttc agttctgcac gggcacgatc ggtgtcagtg
    42841 ttccacaatg tgcgcatgtc catctggtcg gtatcgttcg ggtcgagcgc caccgcctgg
    42901 tgaccaatga tcgcctgata atcctggaag tccgtaatgc ggaaatcacg taacagtgca
    42961 gtgccttcga agcgcgttcc ctggttgaga gtgacgacct gggagccgtc gcgcagctgg
    43021 gttaaatgtc cggaatcggc caccaccaca gaaggacgtg cattaccttt tggtcgaatt
    43081 tgcgcgagga acacatcttt gaaatcgctg ccgtcaacgc tttcgatgaa cagcaccgag
    43141 ctgccattag tcgcttgctg gaattgccct tgcgccagcg ccgccatgcc agggttcgct
    43201 ttcgcttctg ctaacacttc atcctgatga cgcgatgacc acggtcccgc ccacatcacg
    43261 ttaaccgccg cgacgattgc cgtgaatacc gcaaggatca ttgccgcttt caccagaacc
    43321 gctttgctca ggccgcaggc atgcattacc gtaatttcac tttcggtata cagtttgccc
    43381 agcgtcatca gcagcccgag gaacaggctt aatggcagga taagctgcgc catttccggc
    43441 acgcccaacc cgagaaggga gagcaccaga ttcgccggaa tatcgccgtc aaccgctgcg
    43501 ccgaggatcc tcactaactt ttgacagaag aagatcaaaa gcaagatgaa gagtatcgcc
    43561 agctggcttt tgagcgtctc ccgcaccaga tatcttatga ttatcacttt aaatacgccc
    43621 gtaaaaactc gtcttttgca ggattttagc ttgtttcatg gcttaaacgt catttattct
    43681 cttgagtcgt cgaaatcgtc gctaagataa ttatactcaa cggattcacc tctcagattt
    43741 tgttctgacg tgccaatgcc gtaataacgt taagattaac acgaagtcat cgcaacagcg
    43801 gacatgagtt acgaaagctt gcaattctat ctgtagccac cgccgttgtc tttaagattc
    43861 aggagcgtag tgcatggagt ttagtgtaaa aagcggtagc ccggagaaac agcggagtgc
    43921 ctgcatcgtc gtgggcgtct tcgaaccacg tcgcctttct ccgattgcag aacagctcga
    43981 taaaatcagc gatgggtaca tcagcgccct gctacgtcgg ggcgaactgg aaggaaaacc
    44041 ggggcagaca ctgttgctgc accatgttcc gaatgtactt tccgagcgaa ttctccttat
    44101 tggttgcggc aaagaacgtg agctggatga gcgtcagtac aagcaggtta ttcagaaaac
    44161 cattaatacg ctgaatgata ctggctcgat ggaagcggtc tgctttctga ctgaactgca
    44221 cgttaaaggc cgtaacaact actggaaagt gcgtcaggct gtcgagacag caaaagagac
    44281 gctctacagt ttcgatcagt tgaaaacgaa taagagcgaa ccgcgtcgcc cgctgcgtaa
    44341 gatggtgttc aacgtgccga cccgccgtga actgaccagc ggtgagcgcg cgatccagca
    44401 cggtctggcg attgccgccg ggataaaagc agcgaaagat ctcggtaata tgccgccgaa
    44461 tatctgtaac gccgcttatc tcgcctctca ggcgcgccag ctggctgaca gctacagcaa
    44521 gaatgtcatc acccgcgtta tcggcgaaca gcagatgaaa gagctgggaa tgcactctta
    44581 tctggcggtc ggtcagggtt cacaaaacga atcgctgatg tcggtgattg agtacagagg
    44641 caacgcgtcg gaagatgcac gcccaatcgt gctggtgggt aaaggtttaa ccttcgactc
    44701 cggcggtatc tccatcaagc cttcagaagg catggatgag atgaagtacg atatgtgcgg
    44761 cgcggcggcg gtttacggcg tgatgcgtat ggtcgcggag ctgcaactgc cgattaacgt
    44821 tattggcgtg ctggcaggct gtgaaaacat gcctggcggg cgtgcctatc gtccgggcga
    44881 tgtgttaacc accatgtccg gtcaaaccgt tgaagtgctg aacaccgatg ccgaaggccg
    44941 cctggtactg tgcgacgtgt taacttacgt tgaacgtttt gagccggaag cagtgattga
    45001 cgtggcgacg ctgaccggtg cctgcgtgat cgcgctgggt catcatatta ctggtctgat
    45061 ggcgaaccat aatccgctgg cccatgaact gattgccgcg tctgaacaat ccggtgaccg
    45121 cgcatggcgc ttaccgctgg gtgacgagta tcaggaacaa ctggagtcca attttgccga
    45181 tatggcgaac attggcggtc gtcctggtgg ggcgattacc gcaggttgct tcctgtcacg
    45241 ctttacccgt aagtataact gggctcacct ggatattgca ggaaccgcct ggcgttctgg
    45301 taaagcaaaa ggcgcaaccg gtcgtccggt agcgttgctg gcacagttcc tgttaaaccg
    45361 cgctgggttt aacggcgaag agtaagtttg cgttttaccc tcaccctaac cctctcccta
    45421 agggagaggg aaccgatcga gcgcaataga aattcattgc tcatctttca gggtgaggaa
    45481 tgcagcaata tttaaatcca ccacaagaag ccccatatat gaaaaacgca acgttctacc
    45541 ttctggacaa tgacaccacc gtcgatggct taagcgccgt tgagcaactg gtgtgtgaaa
    45601 ttgccgcaga acgttggcgc agcggtaagc gcgtactcat cgcctgtgaa gatgaaaagc
    45661 aggcttaccg gctggatgaa gccctgtggg cgcgtccggc agaaagcttt gttccgcata
    45721 atttagcggg agaaggaccg cgcggcggtg cgccggtgga gatcgcctgg ccgcaaaagc
    45781 gtagcagcag cccgcgcgat atattgatta gtctgcgaac aagctttgca gattttgcca
    45841 ccgctttcac agaagtggta gacttcgttc cttatgaaga ttctctgaaa caactggcgc
    45901 gcgaacgcta taaagcctac cgcgtggctg gtttcaacct gaatacggca acctggaaat
    45961 aatggaaaag acatataacc cacaagatat cgaacagccg ctttacgagc actgggaaaa
    46021 gcagggctac tttaagccta atggcgatga aagccaggaa agtttctgca tcatgatccc
    46081 gccgccgaac gtcaccggca gtttgcatat gggtcacgcc ttccagcaaa ccatcatgga
    46141 taccatgatc cgctatcagc gcatgcaggg caaaaacacc ctgtggcagg tcggtactga
    46201 ccacgccggg atcgctaccc agatggtcgt tgagcgcaag attgccgcag aagaaggtaa
    46261 aacccgtcac gactacggcc gcgaagcttt catcgacaaa atctgggaat ggaaagcgga
    46321 atctggcggc accattaccc gtcagatgcg ccgtctcggc aactccgtag actgggagcg
    46381 tgaacgcttc actatggacg aaggcctgtc caatgcggtg aaagaagttt tcgttcgtct
    46441 gtataaagaa gacctgattt accgtggcaa acgcctggta aactgggacc cgaaactgcg
    46501 taccgctatc tctgacctgg aagtggaaaa ccgcgaatcg aaaggttcga tgtggcacat
    46561 ccgctatccg ctggctgacg gcgcgaaaac cgcagatggt aaagattatc tggtggttgc
    46621 gactacccgt ccggaaaccc tgctgggcga taccggcgtg gccgttaacc cggaagatcc
    46681 gcgttacaaa gatctgattg gcaaatatgt cattctgccg ctggttaacc gtcgtattcc
    46741 gatcgttggc gacgaacacg ccgacatgga aaaaggcacc ggctgcgtga agatcacccc
    46801 ggcgcacgac tttaacgact atgaagtggg taaacgtcac gccctgccga tgatcaacat
    46861 cctgaccttt gacggtgata tccgtgaaag cgcccaggtg ttcgatacca aaggtaacga
    46921 atctgacgtt tattccagcg aaatcccggc agagttccag aaactcgagc gttttgccgc
    46981 acgtaaagcg gtcgttgctg cggttgacgc actcggcctg ctggaagaaa ttaaaccgca
    47041 cgacctgacc gttccttacg gcgaccgtgg cggcgtagtt atcgaaccga tgctgaccga
    47101 ccagtggtac gtgcgtgccg atgtcctggc gaaaccggcg gttgaagcgg ttgagaacgg
    47161 cgacattcag ttcgtaccga agcagtacga aaacatgtac ttctcctgga tgcgcgatat
    47221 tcaggactgg tgtatctctc gtcagctgtg gtggggtcac cgtatcccgg catggtacga
    47281 cgaagcgggt aacgtttatg ttggccgcaa cgaagacgaa gtgcgtaaag aaaataacct
    47341 gagcgcggac gtcgcgctgc gtcaggacga agacgttctc gacacctggt tctcctccgc
    47401 gctgtggacc ttctccacgc tcggctggcc ggaaaacacc gacgcactgc gccagttcca
    47461 cccaaccagc gtgatggtat ccggcttcga catcatcttc ttctggattg cccgcatgat
    47521 catgatgacc atgcacttca tcaaagatga aaatggcaaa ccgcaggtgc cgttccacac
    47581 cgtttacatg accggcctga ttcgtgatga cgaaggccag aagatgtcca aatccaaggg
    47641 taacgtgatt gacccgctgg atatggttga cggtatttcg ctaccggaac tgctggaaaa
    47701 acgtaccggc aatatgatgc agccgcagct ggcggacaaa atccgtaagc gcaccgagaa
    47761 gcagttcccg aacggcattg agccgcacgg tactgacgcg ctgcgcttca ccctggcggc
    47821 gctggcttct accggtcgtg acatcaactg ggatatgaag cgtctggaag gctaccgtaa
    47881 cttctgtaac aagctgtgga acgccagccg ctttgtgctg atgaacacag aaggtcagga
    47941 ttgcggcttc aacggcggcg aaatgacgct atcgctggcg gaccgctgga ttctggcgga
    48001 gttcaaccag accatcaaag cgtaccgcga agcgctggac agcttccgct tcgatatcgc
    48061 cgcaggcatt ttgtatgagt tcacctggaa ccagttctgt gactggtatc tggagctgac
    48121 caagccggta atgaacggtg gaacagaagc cgaactgcgc ggtactcgcc atacgctggt
    48181 gactgtactg gaaggtctgc tgcgcctcgc gcatccgatc attccgttca tcaccgaaac
    48241 catctggcag cgtgtgaaag tactttgcgg tatcactgcc gacaccatca tgctgcagcc
    48301 attcccgcag tatgatgcat ctcaggttga tgaagccgca ctggccgaca ccgagtggct
    48361 gaaacaggcg atcgttgctg tacgtaacat ccgtgcagaa atgaacatcg cgccgggcaa
    48421 accgctggag ctgttgcttc gtggttgcag cgcggatgca gagcgtcgcg taaatgaaaa
    48481 ccgtggcttc ctgcaaacgc tggcgcgtct ggaaagcatc accgtgctgc ctgccgatga
    48541 caaaggtccg gtttccgtta ccaagatcat cgacggcgca gagctgctga tcccgatggc
    48601 tggcctcatc aacaaagaag atgagctggc gcgcctggcg aaagaagtgg cgaagatcga
    48661 aggtgaaatc agccgcatcg agaacaagct ggcaaatgaa ggctttgtcg cccgcgcacc
    48721 ggaagcggtt atcgcgaaag agcgtgagaa gctggaaggt tatgcagaag cgaaagccaa
    48781 gctgattgaa cagcaggctg ttatcgccgc actgtaatca acacagtgaa gaaaacgaag
    48841 gccggagcat gctccggcct tttttatctc ttacagaaat ggtaaagaag gcataactcc
    48901 ccgagaaata ctggctaaga agcctttatc tggttgttct tctttattga ttaaaggaag
    48961 gtcatccaaa tcgcccagca aatacgtatt ttttgcctgc cagtcaatca tccatatttt
    49021 cagcagtgga taagccagac cgcccgtaat cccggatatc accaccaacg cacacatgcg
    49081 ataaagcata ccgtggtacg ttaaagttga gcgaaaacga atacgcccat cattcagtga
    49141 caggttgctc ataaaatgat ttcgcaacga caccgttaaa taactggtgc taacagcaat
    49201 cccaaaataa tagattaact gcgcgattat cattttacgt tgcatttcca taaattgctg
    49261 taaattctca atgtcatcat ttgcatatac agaactatca tacgcattta atatttgatc
    49321 gaagataata taaccagcaa cagcgagaaa aggcaataaa gctaaaaatg cgagaatggc
    49381 atattttata caatacgtag tatctaattt caccttgaaa cgatgtatac cgaaactggt
    49441 attgctccag agaaagctca ttaccaggct atataaagta ccattaaaaa taccaatgga
    49501 aacaatacca actattgcca tcagaaccac agatataata acactacttg aactattggc
    49561 aggtagcatc tttgtcgaga taaagaaaac agtccccatc ccaatggcca ttaaaatcgg
    49621 caagaaaaag gttacccacc agaacccttt caaagagcaa ttaaaactaa atcttacgcc
    49681 gttgagactg gtcattaagg cctgataacg cagtcctttt gctgccataa atataagaaa
    49741 aaccaacagt aacaaagtca aaacacagcc aacaagcggc atatctgctg acactgtcat
    49801 taagattgcg aaatagaaaa aaacaaaaac aagacaacta acaaaaacat tcccaccggt
    49861 aattccataa gaaaatcgtt gtccgttaac ttccatatta gcataaagat aacgcttaca
    49921 tttcattaat gcccatggta aatagatacc tagcgttata atcgttaaca acacattcac
    49981 cacacaaata agaaaatatc tctcacctgt accatgaaaa acaaaagaat gggacggaac
    50041 atccatttca ttaataactt gagccataaa aattccctga attaaaaaat ccattgatat
    50101 aatttataga tacagacagt aaaatgttct gccacgttac aatactctta tcagtaatag
    50161 gctggcaaaa actttattgc tttataagac gcttgcgcac gtaagaagag agtggtatta
    50221 aacaaatgta taattacttt atttttattg ggttaaacta tgaataacat tgcgccgcaa
    50281 tcacctgtaa tacgtcgcct gacgctgcag gataatcctg ctatcgcccg cgtcattcgt
    50341 caggtatccg ccgaatacgg tcttaccgct gataaaggct acaccgtcgc cgatccgaat
    50401 cttgacgagc tgtatcaagt gtatagtcag cctggccatg catattgggt ggttgagtac
    50461 gagggtgaag tggtcggcgg cggtgggata gcgccattaa caggtagtga agcggacatt
    50521 tgcgaactgc agaaaatgta ctttcttccc gccattcgcg gtaaagggct ggcaaaaaaa
    50581 ctggccttaa tggcgatgga gcaggcgcga gaaatgggtt tcaaacgctg ctatctggaa
    50641 acgaccgctt ttttgaaaga agcgattggt ttatatgaac atttaggttt tcaacatatc
    50701 gactatgcac ttggctgcac gggccatgtt gattgtgaag tgcggatgtt gcgcgaactc
    50761 tgaaaaaatg cccgctagta tgtgctatcg ggcattttaa tcaattaatt agtggcgaac
    50821 cccgtcatcg tcttcatcga caaaatcttc atcgtcgcca tcttcgccgt taggatcttc
    50881 aaagtaagtg ccccaaccgt cgtactcaac gtcaaatttc tccgccagcg tcatcagttg
    50941 ttcaacctgg gcatcgatca gatcggcatt caacgcgcac tcgctgagga tgtcgcagca
    51001 aatcacgata tcaccatctt caacttccag ctcttctgga tcggtcactt cgtaaccgag
    51061 tttaaacgct tcaactgccg ctttttccag agtttccaga tcgtctgcgg aaagatggtg
    51121 ttcgatggtg tacagcgcgt ccggatcgct gccatcttcc agtaattctt caataatcaa
    51181 acgtgtttct tcacgctgtt cttccagttg ttccgggttt gccatggctc attcctctta
    51241 atgtcctgcc gatactctta ttgtcacaca ccactgacat tgcctccacc tttgtgataa
    51301 agatttatgc tttagacttg caaatgaata atcatccata taaattgaat tttaattcat
    51361 tgaggcgtta gccacaggag ggatctatgt ccgggtttta tcataagcat ttcctgaaat
    51421 tactcgattt cacgccagct gaacttaaca gcctgctgca gttagccgcg aagctgaaag
    51481 ctgataagaa aagcggtaaa gaagaagcca aactcactgg taaaaacatc gcgctcatct
    51541 tcgaaaaaga ctcgacccgt acccgatgct ctttcgaagt tgccgcatat gaccagggtg
    51601 ctcgcgttac ttatctcggc ccaagcggta gtcagattgg tcataaagag tcgattaaag
    51661 acactgcccg cgtgcttggt cgcatgtatg acggtattca gtatcgcggc tatggtcagg
    51721 agattgtcga aacactggcg gaatacgctg gcgtgccggt atggaacggc ctgaccaatg
    51781 agtttcaccc cacgcagttg ctggcggatc tcctcaccat gcaggagcat ttacccggta
    51841 aagcattcaa cgaaatgacg ctggtctatg caggtgacgc acgtaacaac atgggcaatt
    51901 cgatgctcga agctgcggcg cttaccggtc tggatttgcg tctggtcgcg ccacaggcat
    51961 gctggccaga agctgcgctg gttacggaat gccgcgccct ggcacaacaa aatggcggga
    52021 atattacgct gacggaagat gtagctaaag gagttgaagg tgctgacttt atctataccg
    52081 atgtatgggt gtcgatgggt gaagcaaaag aaaaatgggc tgaacgcatt gcattactgc
    52141 gtgattatca ggtgaacagc aagatgatgc agttgaccgg taacccggag gtcaaattcc
    52201 tccactgcct gcccgcgttt catgacgacc agacgacgct tggtaagaaa atggcggaag
    52261 agtttggcct acatggcgga atggaagtga ctgatgaggt cttcgaatct gccgccagca
    52321 ttgtttttga tcaggcggaa aaccgcatgc ataccatcaa agcggtgatg gtcgcgacgc
    52381 tcagtaaatt aaataattaa tatctctata aagatgccgg ga
//
LOCUS       DAZGQL010000022        49246 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.022, whole genome shotgun sequence.
ACCESSION   DAZGQL010000022 DAZGQL010000000
VERSION     DAZGQL010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 49246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49246
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.022"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..283
                     /locus_tag="ACHKIR_001530"
     CDS             <1..283
                     /locus_tag="ACHKIR_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HFO9989282.1"
                     /translation="INILFTSRQMIIETFICPVSSISNPDEFNTFLLRNQKMMPLSSV
                     GISSVQHEEYYIVFGALSLKSSLEDILLEITSLVDNALDLAEITEEYSH"
     gene            302..1000
                     /gene="yjfJ"
                     /locus_tag="ACHKIR_001531"
     CDS             302..1000
                     /gene="yjfJ"
                     /locus_tag="ACHKIR_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HFO9989283.1"
                     /translation="MGILKSLFTLGKSFISQAEESIEETQGVRMLEQHIRDAKAELDK
                     AGKSRVDLLARVKLSHDKLKDLRERKASLEARALEALSKNVNPSLINEVAEEIARLEN
                     LITAEEQVLSNLEVSRDGVEKAVTATAQRIAQFEQQMEVVKATEAMQRAQQAVTTSTV
                     GASSSVSTAAESLKRLQTRQAERQARLDAAAQLEKVADGRDLDEKLAEAGIGGSNKSS
                     AQDVLARLQRQQGE"
     gene            1050..1709
                     /gene="yjfK"
                     /locus_tag="ACHKIR_001532"
     CDS             1050..1709
                     /gene="yjfK"
                     /locus_tag="ACHKIR_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfK family protein"
                     /protein_id="HFO9989284.1"
                     /translation="MSGFFQRLFGKDNKPAIARGPLGLHLNSGFTLDTLAFRLLEDEL
                     LIALPGEEFTVAAVSRIDLGGGSQIFRYYTSGDEFLQINTTGGEDIDDIDDIKLFVYE
                     ESYGISKESHWREAINAKAMGAMTLNWQEKRWQRFFNSEEPGNIEPVYMLEKVENQNH
                     AKWEVHNFTMGYQRQVTEDTYEYLLLNGEESFNDLGEPEWLFLRALGVDIPLTSLHII
                     G"
     gene            1727..2125
                     /gene="yjfL"
                     /locus_tag="ACHKIR_001533"
     CDS             1727..2125
                     /gene="yjfL"
                     /locus_tag="ACHKIR_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HFO9989285.1"
                     /translation="MHILDSLLAFSAYFFIGVAMVIIFLFIYSKITPHNEWQLIKNNN
                     TAAALAFSGTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPALSE
                     KIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            2135..2773
                     /gene="yjfM"
                     /locus_tag="ACHKIR_001534"
     CDS             2135..2773
                     /gene="yjfM"
                     /locus_tag="ACHKIR_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HFO9989286.1"
                     /translation="MARKRKSRNNKNIGHGAISRIGRPHNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCGDSGDVDNDGDGTFYATVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRSGSGKKESYSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            2776..3939
                     /gene="yjfC"
                     /locus_tag="ACHKIR_001535"
     CDS             2776..3939
                     /gene="yjfC"
                     /locus_tag="ACHKIR_001535"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFO9989287.1"
                     /translation="MLRHNVPVRRDLDQIAADNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGVIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAEQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFDGEKPQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            4023..5648
                     /gene="aidB"
                     /locus_tag="ACHKIR_001536"
     CDS             4023..5648
                     /gene="aidB"
                     /locus_tag="ACHKIR_001536"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HFO9989288.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPAPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERMEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTTGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCL"
     gene            complement(5756..6031)
                     /gene="yjfN"
                     /locus_tag="ACHKIR_001537"
     CDS             complement(5756..6031)
                     /gene="yjfN"
                     /locus_tag="ACHKIR_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protease activator YjfN"
                     /protein_id="HFO9989289.1"
                     /translation="MKQFLASSSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(6180..6509)
                     /gene="bsmA"
                     /locus_tag="ACHKIR_001538"
     CDS             complement(6180..6509)
                     /gene="bsmA"
                     /locus_tag="ACHKIR_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HFO9989290.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            6691..7440
                     /gene="yjfP"
                     /locus_tag="ACHKIR_001539"
     CDS             6691..7440
                     /gene="yjfP"
                     /locus_tag="ACHKIR_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO9989291.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCAIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDAARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLRDDRLAVGGASMGAMTALGITARHSTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNNIVAPLAEWEATNHLEQLADRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            complement(7437..8192)
                     /gene="ulaR"
                     /locus_tag="ACHKIR_001540"
     CDS             complement(7437..8192)
                     /gene="ulaR"
                     /locus_tag="ACHKIR_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HFO9989292.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(8300..9364)
                     /gene="ulaG"
                     /locus_tag="ACHKIR_001541"
     CDS             complement(8300..9364)
                     /gene="ulaG"
                     /locus_tag="ACHKIR_001541"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFO9989293.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADILRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            9719..11116
                     /gene="ulaA"
                     /locus_tag="ACHKIR_001542"
     CDS             9719..11116
                     /gene="ulaA"
                     /locus_tag="ACHKIR_001542"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFO9989294.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASWIAYKFAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVNWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            11132..11437
                     /gene="ulaB"
                     /locus_tag="ACHKIR_001543"
     CDS             11132..11437
                     /gene="ulaB"
                     /locus_tag="ACHKIR_001543"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HFO9989295.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKEHFPQDVK"
     gene            11447..11911
                     /gene="ulaC"
                     /locus_tag="ACHKIR_001544"
     CDS             11447..11911
                     /gene="ulaC"
                     /locus_tag="ACHKIR_001544"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HFO9989296.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            11925..12575
                     /gene="ulaD"
                     /locus_tag="ACHKIR_001545"
     CDS             11925..12575
                     /gene="ulaD"
                     /locus_tag="ACHKIR_001545"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFO9989297.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIGQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            12585..13439
                     /gene="ulaE"
                     /locus_tag="ACHKIR_001546"
     CDS             12585..13439
                     /gene="ulaE"
                     /locus_tag="ACHKIR_001546"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HFO9989298.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLQLAKTLGFDFVEISVDETD
                     DRLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVAKARDWVKARMA
                     KAGMVEAA"
     gene            13439..14125
                     /gene="ulaF"
                     /locus_tag="ACHKIR_001547"
     CDS             13439..14125
                     /gene="ulaF"
                     /locus_tag="ACHKIR_001547"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HFO9989299.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGDAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARGINPQLNHIDSYLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(14254..14529)
                     /gene="yjfY"
                     /locus_tag="ACHKIR_001548"
     CDS             complement(14254..14529)
                     /gene="yjfY"
                     /locus_tag="ACHKIR_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YjfY"
                     /protein_id="HFO9989300.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            14857..15252
                     /gene="rpsF"
                     /locus_tag="ACHKIR_001549"
     CDS             14857..15252
                     /gene="rpsF"
                     /locus_tag="ACHKIR_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFO9989301.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            15259..15573
                     /gene="priB"
                     /locus_tag="ACHKIR_001550"
     CDS             15259..15573
                     /gene="priB"
                     /locus_tag="ACHKIR_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HFO9989302.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            15578..15805
                     /gene="rpsR"
                     /locus_tag="ACHKIR_001551"
     CDS             15578..15805
                     /gene="rpsR"
                     /locus_tag="ACHKIR_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFO9989303.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            15847..16296
                     /gene="rplI"
                     /locus_tag="ACHKIR_001552"
     CDS             15847..16296
                     /gene="rplI"
                     /locus_tag="ACHKIR_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFO9989304.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            16646..17137
                     /locus_tag="ACHKIR_001553"
     CDS             16646..17137
                     /locus_tag="ACHKIR_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO9989305.1"
                     /translation="MGKKTAPLLPYTNQMLSEFGERLKLARLRRRLTAKQVAERAGMS
                     PITLRSLESGSAGVTIGAYLSVMQVLGLETDLNKLAAEDDLGRQLQDSRLIKKRGLSR
                     ASSKNEEGSQTASLQMRHEKEGHLTTDLTALAEGSTPSITGETSTSLAALLKPIKKPL
                     KGN"
     gene            17140..18408
                     /locus_tag="ACHKIR_001554"
     CDS             17140..18408
                     /locus_tag="ACHKIR_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family
                     toxin"
                     /protein_id="HFO9989306.1"
                     /translation="MALIGVYADWEGLDGPERIGYLHSRRTRTREIFEFEYDKKALAD
                     PSLNFIQLDPEIMLYEGAQYPIPPKDKFGAFSDSCPDRWGRMLMKRRFERDIRDGLCD
                     KDSHLYESDYLLGVHDLYRVGALRYKREDAGEFLDNRIDVAAPPFTEIASLERASRAI
                     EEDPDNKELMGQKWLRMLIAPGGSLGGARPKASVVDEAGHLYIAKFPSVKDEYDVGGW
                     EMAVNALAVGCGLNVAPAQAHKFASNYHCFMVRRFDRTNAGRRLHFASAMTLTRHQDG
                     EDASTGVSYLELADVLIRHGAQTNTDLKELWSRIVFNILVSNTDDHLRNHGFILIPGK
                     GWRLSEAYDLNPVVRSDGLKLNITENDNALDLELAREVAEYFRLSLTEADDIIANFRG
                     IVSQWRIIAERLRLPGREQELMAEAFRASI"
     gene            complement(18551..19828)
                     /locus_tag="ACHKIR_001555"
     CDS             complement(18551..19828)
                     /locus_tag="ACHKIR_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9989307.1"
                     /translation="MNKIKNYRWHMIALVCFITVINYLDRTALGIAAPTIMETTGITK
                     EQYSWIVSAFQLAYTLGQPIMGFFIDTVGLKLSFAICAAIWGLATMGHALTGTWSGLA
                     FMRALMGFSEASAIPAGVKTASTWFPAKERGVATGVFNMGTSLGAMLAPPLIAWCIMF
                     HSWQFAFIVSGSLALLAALFWFFCYKDPKDAKRLSDEERHYIESGQEQHLKTDKKEKT
                     SIKHILSQRNFWGIGIARFLADPAWGTINFWVPIFFVETLHFSLKEIAMFVWLPFLLG
                     DLGCLASGFVAKFFHDRGVSLINSRRITFTIAAVIMMTIGLVSIVENPYIAVLLISIG
                     AFSHQCLSTVAATLGGDLFKKDEVATAVGMAGACAWSGQLIFNLFIGAFVHIIGFAPF
                     FIALAFFDIIGAIALWMLIKVKDEEPQVQLATS"
     gene            complement(19921..21993)
                     /locus_tag="ACHKIR_001556"
     CDS             complement(19921..21993)
                     /locus_tag="ACHKIR_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HFO9989308.1"
                     /translation="MSQLDDTILDALTHVTFPKGFAQAEPAWVVTVDGVDYPLWQTDA
                     LVVGSGAAGLRAAVELKRRQQNVLIATAGLYMGTSACSGSDKQTLFTAATAGNGDNFT
                     KLAEALASGGAMDHDTAYVEAVGSLHTLGGLQYLGLELPEDRYGAILRYQTDHDEAGR
                     ATSCGPRTSRLMVKVLLEEVQRLAIPMLTSATVIKLLHQRDENGEDRVAGAILATGHR
                     VHNPWGLAIVIAPNVVLATGGPGELYRDSVYPHKCFGSLGLALEEGLTLTNLTESQFG
                     IGTPRSTFPWNLSGTYVQVIPHIYSVDAGGNEYNFLADYYRTTQELASNIFRKGYQWP
                     FHATRVMEFGSSLLDMAVAQEQQSGRQVFMDFNRNPEAVPGDLPFSLDRLDDDVRAYL
                     ENNDALAPSPIERLQRMNPLSISLYKMHGYDLTTQPLQFAMNNQHMNGGIEVDIWGQT
                     SLPGCFAVGEVAGTHGVTRPGGAALNAGQVFAVRLARFIGCTQKRNIDGDIAQLVAPA
                     LASIREIITQAHDNGTGMPLSVVREKIQARMSDYAGFICHADKVRRATRDALLLNEFV
                     QRHGLAIKHVGEVAELFMWRHMALTSAAVLTQLTHYIDAGGGSRGARMVIDPQGKCLP
                     QTRRGAKEEWRFRSELAEDKNHKLTIQYSQGSFITEVKSLRMQPCINGIYFEKNWPDF
                     LKGDIYTQ"
     gene            complement(21990..23531)
                     /locus_tag="ACHKIR_001557"
     CDS             complement(21990..23531)
                     /locus_tag="ACHKIR_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA--acetoacetyl-CoA transferase"
                     /protein_id="HFO9989309.1"
                     /translation="MRKLSTAETLAAQIQDGATIAISGNGGGMVEADHIMAAIEARFL
                     QTGHPRDLTLIHSLGIGDRDSKGTNRFAHAEMLKRIIAGHFTWSPKMQELVKSNAIEA
                     YCFPGGVIQALLREIGAGRPGLFTHVGLGSFVDPRNGGGKSNECTTDDLVELIEIDGE
                     TKLRYRPFKVDYAILRGTYADPRGNVSLEEEAIDMDSYSMALAAHNSGGKVFVQVRDV
                     LEAGAIEPRKVKLPGILVDGIVEHREQPQTYLGGYDLTISGQHRRLSSNDAIELVSHP
                     VRRLIARRAARELVAGASTNFGFGIPGGIPGVALREGVPYQSLWMSVEQGVHNGMMLD
                     DALFGCARNADAIIPSLDQFEFYSGGGIDITFLGMGEMDRYGNVNVSHLNGNLIGPGG
                     FLEIAQNARKVVFCGTFDAKGSKIDVTPDGLHIAQSGQIPKLVTQVEKITFSAAYAQQ
                     SGQEVLYITERAVFQLTAEGVELIEIAPGVEIERDILPYMAFRPIIKHPRLMESSLFT
                     PMEDA"
     gene            complement(23541..24317)
                     /locus_tag="ACHKIR_001558"
     CDS             complement(23541..24317)
                     /locus_tag="ACHKIR_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HFO9989310.1"
                     /translation="MSDQPVLFSRAAASCRLTLNREDKCHAINEEMIESLDHYLNEIE
                     NDTTLRLVELTATGDKFFCAGGDIKSWSAYSPLDMGRKWIKRGNEVFNRLRNLPQLTV
                     ANLNGHTIGGGIELALCCDIRIARPGAKFSNPEVMLGMVPGWMGIERVLNQVGPVVGR
                     QMLMLGKRLTAQEAQVANLIDEVVEKEQVESWMANQLAQLEKCGPVALAHIKQLILAL
                     ENKHADYSHQLLAGLMSATQDCQQATHAFAEKAGVSFHNQ"
     gene            complement(24327..25169)
                     /locus_tag="ACHKIR_001559"
     CDS             complement(24327..25169)
                     /locus_tag="ACHKIR_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFO9989311.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQGEDLQQIARQLAASNINVSGLCRSTYYTAPTLAERKLAIDDNRRALDDAAVLNAA
                     CYMQVVGGLPMGTKDLYEAREQVKQGIRQLLPHSKEVGVPIALEPLHPMTAADRSCLC
                     TLRQALDWCDELDPDGEFGLGVAVDVYHVWWDPDLASQILRAGKRILAFHVSDWLVPT
                     TDLVNDRGMPGDGVINIPSIRRLVENAGFNGAIELEIFSPYWWQKDINSTLDISVDRI
                     AHYC"
     gene            complement(25179..25970)
                     /locus_tag="ACHKIR_001560"
     CDS             complement(25179..25970)
                     /locus_tag="ACHKIR_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HFO9989312.1"
                     /translation="MKSTRPVAVITGAARGIGKGCALELARGGFNLLINDRPDADSVE
                     KLHATQQECLAEGVEVICFPADVGDLSLHEEMLDAAQNQWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            26183..26830
                     /locus_tag="ACHKIR_001561"
     CDS             26183..26830
                     /locus_tag="ACHKIR_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFO9989313.1"
                     /translation="MYLDTSNVQSLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     QTVYHHFGNKENLFIEVLEYTWNDIRQKEKALDFSDLPPQKAIEKIIDFTWDYYISNP
                     WFLKIVHSENQSKGVHYAKSQRLLEINHAHLQLMESLLDEGKKNNIFKPDIDPLQVNI
                     NIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILSWLLVDPSSTARE"
     gene            complement(26814..27452)
                     /gene="ytfB"
                     /locus_tag="ACHKIR_001562"
     CDS             complement(26814..27452)
                     /gene="ytfB"
                     /locus_tag="ACHKIR_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HFO9989314.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTTHTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GADKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            27670..28290
                     /gene="fklB"
                     /locus_tag="ACHKIR_001563"
     CDS             27670..28290
                     /gene="fklB"
                     /locus_tag="ACHKIR_001563"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFO9989315.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            28599..30011
                     /gene="cycA"
                     /locus_tag="ACHKIR_001564"
                     /gene_synonym="dagA"
     CDS             28599..30011
                     /gene="cycA"
                     /locus_tag="ACHKIR_001564"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HFO9989316.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVLVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(30056..30718)
                     /gene="ytfE"
                     /locus_tag="ACHKIR_001565"
     CDS             complement(30056..30718)
                     /gene="ytfE"
                     /locus_tag="ACHKIR_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HFO9989317.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVDVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMEHISLENNVLFPRALA
                     GE"
     gene            complement(30826..31791)
                     /gene="ytfF"
                     /locus_tag="ACHKIR_001566"
     CDS             complement(30826..31791)
                     /gene="ytfF"
                     /locus_tag="ACHKIR_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO9989318.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALVCIGIGLACVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNTQ
                     TPGFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVVGVVIAVRAKPEKPLTESVSES"
     gene            complement(31899..32759)
                     /gene="qorB"
                     /locus_tag="ACHKIR_001567"
     CDS             complement(31899..32759)
                     /gene="qorB"
                     /locus_tag="ACHKIR_001567"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFO9989319.1"
                     /translation="MIAITGATGQLGHYVIESLMKTVPASQIVAIVRNPAKAQALTAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADSSPLGLADEHIETEKMLADSGIVYTLLRNGWYTENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAVAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDDSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            32848..33228
                     /gene="ytfH"
                     /locus_tag="ACHKIR_001568"
     CDS             32848..33228
                     /gene="ytfH"
                     /locus_tag="ACHKIR_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFO9989320.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKMGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            complement(33357..35300)
                     /gene="cpdB"
                     /locus_tag="ACHKIR_001569"
     CDS             complement(33357..35300)
                     /gene="cpdB"
                     /locus_tag="ACHKIR_001569"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008663 - 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HFO9989321.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDIHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIA
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAGDKKLDIRFETSPSDKAAVFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            35490..36230
                     /gene="cysQ"
                     /locus_tag="ACHKIR_001570"
     CDS             35490..36230
                     /gene="cysQ"
                     /locus_tag="ACHKIR_001570"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HFO9989322.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAVAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            complement(36220..36777)
                     /gene="ytfJ"
                     /locus_tag="ACHKIR_001571"
     CDS             complement(36220..36777)
                     /gene="ytfJ"
                     /locus_tag="ACHKIR_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001498728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFO9989323.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLHKLINK"
     gene            37102..37308
                     /gene="ytfK"
                     /locus_tag="ACHKIR_001572"
     CDS             37102..37308
                     /gene="ytfK"
                     /locus_tag="ACHKIR_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HFO9989324.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            complement(37370..38713)
                     /gene="paeA"
                     /locus_tag="ACHKIR_001573"
     CDS             complement(37370..38713)
                     /gene="paeA"
                     /locus_tag="ACHKIR_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFO9989325.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANI
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(39036..39674)
                     /gene="msrA"
                     /locus_tag="ACHKIR_001574"
     CDS             complement(39036..39674)
                     /gene="msrA"
                     /locus_tag="ACHKIR_001574"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFO9989326.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRRITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            39880..41613
                     /gene="tamA"
                     /locus_tag="ACHKIR_001575"
     CDS             39880..41613
                     /gene="tamA"
                     /locus_tag="ACHKIR_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HFO9989327.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQILDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSFDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            41610..45389
                     /gene="tamB"
                     /locus_tag="ACHKIR_001576"
     CDS             41610..45389
                     /gene="tamB"
                     /locus_tag="ACHKIR_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HFO9989328.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSMPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEAAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYAISMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEIPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHELPESAVGVSSDVVMLNDNLQPEEAKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            45392..45733
                     /gene="ytfP"
                     /locus_tag="ACHKIR_001577"
     CDS             45392..45733
                     /gene="ytfP"
                     /locus_tag="ACHKIR_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HFO9989329.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(45852..46847)
                     /locus_tag="ACHKIR_001578"
     CDS             complement(45852..46847)
                     /locus_tag="ACHKIR_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO9989330.1"
                     /translation="MSIKKLADGKYCVDVRPAGSEGRRFRRRFPTRGEAAMYERHVLT
                     HYHDKDWVEKPIERKALSDLLELWWVYHGKNHRYGAMNRVRIEAVLRDMQELGINRSD
                     HLTRKNIIRYRLELMNRGIKQSTVNRYCAMMSGFFEKLIDVEEFVGDNPFHEIRKLTI
                     NQPEMAYLAHDDIPRLTALLSGDNLKAVLLSLATGGRWGEVANIKGEHIIGGKVIFME
                     TKNGSRRVIPIAKALESLIKVKATGRLMNPSYAIVRSAIRTVRPDLPVGQSVHVLRHT
                     FATHFMINGGNIITLQRILGHSTIQQTMTYAHFAPDYLQDAVRFNPVAELSRFCP"
     gene            complement(46914..47213)
                     /locus_tag="ACHKIR_001579"
     CDS             complement(46914..47213)
                     /locus_tag="ACHKIR_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO9989331.1"
                     /translation="MSIPQNEKLKLIRESERMKMKELSDLIGINYYTYHGYESGKSKM
                     PLDFGILLLRHARFRKYRDWFLFDEVEPKAGQVVPALAHIGQSEAESSHSGKKTG"
     gene            47321..47746
                     /locus_tag="ACHKIR_001580"
     CDS             47321..47746
                     /locus_tag="ACHKIR_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cox family DNA-binding protein"
                     /protein_id="HFO9989332.1"
                     /translation="MQEVTQNAPESPAETQCDQLTDAPETAHQKTGRKAPERQEIRLS
                     QNPSHLLSKEGFAMYIGKTVDAIVSMAKAGKLPAVYMADPLKPGGNAELYINRKAWDE
                     ACDRLTENAPQEWHEWENRLFLFKPAAGSRNKRISKKAA"
     gene            47777..48268
                     /locus_tag="ACHKIR_001581"
     CDS             47777..48268
                     /locus_tag="ACHKIR_001581"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9989333.1"
                     /translation="MIKKYSEHGSKAGEAIAIDEDCLITGDPWLATERDLEGKTFFVR
                     AIKHKDRVVGGIIYSTSTIIPDNYLDISFRPVSIQGCEAYLNYVAELKKINLDYMWPI
                     VQREIMRRKSEGFDVKIEFLSTLMGFLQKPLILFPSLDRNLPVKEGVEKYIVEQVRDL
                     VCL"
     gene            complement(48246..48569)
                     /locus_tag="ACHKIR_001582"
     CDS             complement(48246..48569)
                     /locus_tag="ACHKIR_001582"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9989334.1"
                     /translation="MIDKSVKDAITEYLVSIYTTNLAVAPVDKLTDPRDSVGVVSDLL
                     ISLLISELPDDKRDALMKTASEILRQIEGQQAFAPYAVNIRTRFHSINHLACFIRASK
                     DKPDL"
     gene            48677..49015
                     /locus_tag="ACHKIR_001583"
     CDS             48677..49015
                     /locus_tag="ACHKIR_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000194243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4761 family protein"
                     /protein_id="HFO9989335.1"
                     /translation="MTQRRSFRTGTERHAARFSTSASRSNSHYSLQETHATPDGYLVK
                     QTGKHTWLINDAGIVIHRGARNPITGNTCYSLTRGDEQFGQDFTLHEALRTADRLISG
                     RSFQHPEHRH"
     gene            49035..>49246
                     /locus_tag="ACHKIR_001584"
     CDS             49035..>49246
                     /locus_tag="ACHKIR_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4754 family protein"
                     /protein_id="HFO9989336.1"
                     /translation="MIEAYKILINNALQRYHFELANRGVTRDEGLRNSLRGAIMGLYN
                     CALGSDDREALEELKVIVAELFEGEIV"
BASE COUNT        12120 a        12092 c        12844 g        12190 t
ORIGIN      
        1 aatcaatatt ctatttactt cccgccaaat gattatcgaa acctttattt gtccggtaag
       61 tagtatcagc aatccagatg aatttaatac cttcttatta agaaatcaga agatgatgcc
      121 gttgtcatcg gtagggatct ccagcgtgca acatgaagag tattacattg ttttcggtgc
      181 gttatcgctt aaatcttctc ttgaagatat cctgcttgag ataacctcgc tggtagataa
      241 cgcattggat ctggctgaaa ttacagaaga atattcacac taattacaag gacgggtatt
      301 tatgggaatt ttaaaaagtt tatttacgct ggggaaatcg tttatctccc aggcggaaga
      361 atccattgaa gaaacccagg gcgtgcgcat gctggaacag catattcgtg acgctaaagc
      421 tgaactcgat aaagccggaa aatctcgcgt tgatctgctg gcgcgggtga aattaagtca
      481 cgataagctg aaagatttac gtgagcgcaa agccagtctg gaagctcgtg cgctggaagc
      541 gttaagcaag aacgttaatc cgtcgttgat taacgaagtt gctgaagaaa tcgcgcgcct
      601 tgagaatctc attaccgctg aagagcaagt gttgtcgaat ctggaagtct ctcgtgatgg
      661 cgtggaaaaa gcagttacag cgacagcgca gcgtatcgct cagtttgagc agcaaatgga
      721 agtcgttaaa gctactgaag ccatgcagcg tgcacaacag gctgtaacga cctctactgt
      781 tggcgcatct tccagcgttt cgacagcggc agaatcctta aaacgcctgc aaacgcgtca
      841 ggccgaacgt caggctcgcc tggatgctgc cgcacagttg gaaaaagtcg cagacggtcg
      901 cgatcttgat gaaaagctgg cggaagccgg aattggcggt agcaataaaa gtagcgccca
      961 ggatgtatta gcaagactgc aacgccaaca gggcgagtaa ttttttgcca gcctcactgc
     1021 aaggctggca actattttaa ggataaaata tgtctggttt tttccagcgt ctgtttggca
     1081 aggataataa gcctgctatc gctcgcggtc ctctgggact tcatctcaat agtggcttta
     1141 cgctcgacac gttagcgttt cgtttgctgg aagatgaatt gctgattgcg ctgccgggtg
     1201 aggaatttac ggtagccgct gtcagccgca tcgatctggg cggcggtagc cagattttcc
     1261 gctactacac ttcaggcgat gagtttctgc aaatcaatac cactggcggc gaagatattg
     1321 atgacattga tgatatcaag ctctttgtct atgaagagag ctacggtatc agtaaagaga
     1381 gtcactggcg cgaggcgatc aatgccaaag cgatgggggc aatgacctta aactggcagg
     1441 aaaaacgctg gcagcgattt tttaatagcg aagaaccggg aaatatcgaa cccgtctaca
     1501 tgctggaaaa agtagaaaat caaaaccatg ctaaatggga ggtccataat tttaccatgg
     1561 gctatcaacg gcaagtgacc gaagatactt acgaatatct gctgttaaat ggtgaggaat
     1621 cttttaatga tctcggcgag ccggagtggt tatttttgcg cgcgctgggg gtcgatatcc
     1681 cgctgacatc acttcatatt attggttaat tacaaggacg tttacgatgc atatactgga
     1741 ttcacttctt gcctttagcg cttatttttt tattggtgtg gctatggtga ttatttttct
     1801 gtttatctac tccaaaatta caccgcacaa cgaatggcag ttaatcaaaa ataataacac
     1861 cgcggcggca ctggcgttca gcggaacatt gttggggtac gtgatcccct tatccagtgc
     1921 ggcaatcaat gcggtgagta ttccagacta tttcgcatgg ggcgggattg ctctggtgat
     1981 tcaattactc gtttttgctg gcgtcaggct ttatatgccc gcattaagcg aaaaaattat
     2041 taatcacaat accgcagcag gaatgtttat gggaaccgcc gcgctggctg gcggtatttt
     2101 taacgcagct tgtatgacat ggtaatggac gatcatggcc agaaaacgca aatccagaaa
     2161 taacaaaaat atcggacatg gggcaatcag tcgcattggt agaccgcata atccttttga
     2221 gccgcgccgc aatcgctacg cacaaaaata cttaacgttg gcgctaatgg gcggtgccgc
     2281 ttttttcgta ttgaaaggtt gtggcgatag tggcgatgtc gataacgacg gcgacggaac
     2341 gttttacgcg actgtgcagg attgtattga tgacggtaat aatgcagaca tttgcgcgcg
     2401 tggctggaac aacgccaaag cggcgtttta tgccgatgtt ccgaagaata tgactcagca
     2461 gaattgtcag tctaagtacg aaaattgcta ctacgacaat gttgaacaga gttggatccc
     2521 ggtcgtttct ggatttttgt taagccgggt tattcgtaaa gatcgcgatg agccgttcgt
     2581 ctataacagc ggcggatcct catttgcttc gcgccccgtc tggcgcaaca cttctggtga
     2641 ttactcctgg cgctccggtt ctggcaaaaa agagtcttac tcttcgggcg gctttaccac
     2701 caaaaaagcg tcaaccgttt ctcgcggcgg ctatggtcgt tcttccagcg cccgtgggca
     2761 ttggggaggc taatcatgct gagacacaac gttcctgtgc ggcgggatct ggaccagatc
     2821 gccgctgaca acggtttcga ctttcatatc atcgacaatg aaatctattg ggacgagagt
     2881 cgagcttacc gttttactct gcgccagatt gaagagcaga tcgaaaaacc gactgcggaa
     2941 ctgcatcaga tgtgccttga ggtggtggat cgcgcggtta aagacgaaga gatcctgacg
     3001 caactggcga tcccgccgtt gtactgggat gtgatcgccg aaagctggcg cgcacgcgat
     3061 ccttcgttgt atggccgtat ggattttgcc tggtgtggta atgcaccggt taagttgctg
     3121 gagtacaacg ccgatacgcc aacttcattg tacgagtcgg cttatttcca gtggttgtgg
     3181 ctggaagatg cccggcgcag cggcgttatt ccgcgcgatg ccgatcagta caatgccatt
     3241 caggagcgcc tgatttcacg ctttagcgag ctttacagtc gggaaccgtt ttatttttgc
     3301 tgctgtcagg acaccgacga agacaggagt accgtgctgt acttgcagga ctgcgccgag
     3361 caggcagggc aggagtcgcg gtttatctac attgaagatc tcggtttggg cgtcggcggt
     3421 gtgctgaccg atcttgatga taatgtcatc cagcgtgcat ttaagctgta tccgctggag
     3481 tggatgatgc gtgacgataa cggtccgctg ctgcgcaagc gtcgcgagca atgggtggag
     3541 ccgttatgga aaagtatctt gagtaataaa gggctaatgc cgctgctttg gcgcttcttc
     3601 cctggtcatc ctaaccttct tgcatcctgg ttcgatggtg aaaaaccgca gattgccgct
     3661 ggcgaaagct atgtgcgtaa accgatttac tcgcgcgaag gcggaaacgt caccattttc
     3721 gacggtcaga ataacgttgt tgaccacgct gatggtgatt acgccgatga accgatgatc
     3781 taccaggcgt ttcaacctct gccgcgattt ggcgatagct acacactcat cggtagctgg
     3841 attgtcgatg atgaagcgtg cggaatgggg atccgtgaag ataacacgtt gatcaccaaa
     3901 gacacctcac gtttcgttcc gcattacatt gctggataag aatgttttag caatctcttt
     3961 ctgtcatgaa tccatggcag tgaccatact aatggtgact gccattgatg gagggagaca
     4021 cagtgcactg gcaaactcac accgttttta atcaacctat accattaaat aacagcaact
     4081 tatacctgtc tgatggcgcg ctctgcgaag cggtaacgcg tgaaggtgct ggctgggata
     4141 gcgattttct cgccagtatt ggtcagcagt tagggacggc tgaatccctt gaactggggc
     4201 ggctggcgaa tgtgaatccg cctgaattat tgcgctacga tgcccagggg cggcgtctgg
     4261 acgatgtgcg ttttcacccc gcctggcacc tgctgatgca ggcgctatgt accaatcggg
     4321 tgcacaatct tgcctgggaa gaagacgctc gctccggcgc atttgtggcg cgcgcggcgc
     4381 gttttatgtt gcacgcacag gttgaggcag ggtcgttatg tccgataacg atgacctttg
     4441 ccgccacgcc attgctgtta cagatgttac ccgcgccgtt tcaggactgg accacgccgc
     4501 tgttgagcga tcgctacgat tctcacttat tgccaggtgg gcaaaaacgc ggtttgttga
     4561 ttggcatggg aatgacggaa aaacagggcg gttccgatgt catgagcaac accacccgcg
     4621 cagagcgcat ggaagatggc tcttatcggc tggtggggca taaatggttt ttctcggtgc
     4681 cgcaaagcga tgcgcatctg gtgctggcgc agactacggg cggtttgtcc tgcttttttg
     4741 tgccgcgctt tttgcctgac gggcaacgca acgcgattcg cctcgagcgg ctgaaagata
     4801 agctgggtaa tcgctctaac gccagctgcg aagtggagtt tcaggatgcc attggctggt
     4861 tgttggggca ggaaggggaa ggaattcgtc tgatcctgaa aatgggtggg atgacgcgtt
     4921 ttgattgcgc cctgggtagc catgccatga tgcgccgtgc attttcgcta gcgatttatc
     4981 atgcacatca acgccatgtt tttggtaatc cattgatcca acagcccctt atgcgtcatg
     5041 tcttaagtcg catggcactt cagcttgaag ggcaaacggc gttgctgttt cgtcttgctc
     5101 gagcgtggga ccggcgtgcc gatgccaaag aagccctgtg ggcgcgttta tttacgcctg
     5161 cggcgaaatt tgtgatctgc aaacgaggta tgccgtttgt ggccgaagcg atggaggtgc
     5221 tgggcggcat tggttattgc gaggagagcg agctgccgcg gctttaccgg gagatgccgg
     5281 taaacagtat ttgggaaggt tccggtaata ttatgtgcct ggatgtactg cgcgtgctca
     5341 ataagcaagc gggtgtatac gacttattgt cggaagcatt tgtggaagtg aaagggcagg
     5401 atcgctattt tgatcgcgcg gttcgtcgtt tacagcagca gctgcgtaag ccagctgaag
     5461 aactggggcg agagattact catcagctat tcctgctggg ctgcggtgcg caaatgttga
     5521 aatatgcctc tccgccaatg gcgcaggcgt ggtgtcaggt gatgttagat acgcgcggcg
     5581 gcgtacggtt gtcagagcag atccagaatg atttattgct gcgggcgacg ggaggagtgt
     5641 gtttgtaagc gtatacgacg ctggtttcga ttaactcaat gaaatatgtg aaaattgtta
     5701 gccggacaag gcgctcgcgc cgcatccggc attgttcatt tgctgtagat gacactcacg
     5761 catacaggat cgcctgtacg cgccagttat caggctgctg gtcttcgtac atggtaataa
     5821 tgcggtacca tgacgcacct tgttcatcag cctttaacgc cacgatacgt tccacgtcct
     5881 gcggatcccc tgaaatattg ctaactgaga tctgacctat ttcattcagg cctgtcacgc
     5941 agtcagcact ggcgaattct gcagactgtg ccgtggcgct cgcaggataa gtcactaatt
     6001 gcagcgagga tgaggcaagg aattgtttca tcgtcagctc catttttcgt ttccccacat
     6061 gttgcagggc attccatcgc ggtaataaac gcccaggctt ccagccggta atttattgtg
     6121 cacaggtaaa cgcataggga cgcaaagcaa tgtaaagctg actatgtaag ttgtctgatt
     6181 tatttacggt acaaaatggc ttgtgaatac cactgtcctg tgacgatggt ttcgtccacc
     6241 atgacgacaa cgtaataatc agcttttgca gcgacagctt tcgctctgat ttctgctaat
     6301 gcgtcatccg gagaaccccg aaccatcgtg cttacgctac ctattcgctg taacccttgc
     6361 gtctggtcgc ggcgaatctc ttgcggatgg tcggttactg gcggtgctgg ctgtggcgta
     6421 ccttgtagtg cgctacaggc acttaacatc aacaccaata ataaactggc aaaccggtaa
     6481 ataacgctat tacgtttcct gctaaccata gtgtagtgcc ttattaattt aactttgggg
     6541 gaatgttccc gaattgttac ctggtacgcg attttcgaat gaaaacgtga tgaaagcgta
     6601 acccacatct caacattatg aactaatacc gccagcagaa acttgtgcta aggatttctt
     6661 catgtcatac cagacaaaaa ggagagacag atgattgaaa tagaatcacg cgagctggca
     6721 gatattcccg ttcttcatgc ttatcctgtc gggcaaaaag ataccccgtt accgtgcgcg
     6781 attttttatc acggctttac ttcgtccagt ctggtgtata gctattttgc cgttgcgctg
     6841 gcgcaggctg gtttgcgggt gatcatgccg gatgcgcccg atcacggtag tcgttttagt
     6901 ggcgacgcag cgcggcggtt aaatcaattc tggcaaatct tgctacaaag tatgcaggaa
     6961 ttcactactt tacgtgcggc aatagccgaa gaaaactggc tgcgtgatga ccgtctggca
     7021 gtcggtggcg cgtcgatggg cgcgatgacg gcactgggaa ttaccgctcg ccactccacg
     7081 gtgaaatgta ccgccagcat gatgggatcg ggctatttta catcgctcgc ccgttcactg
     7141 tttccaccgc tgatacctga aacggcagca cagcagaatg aattcaataa cattgtcgcg
     7201 ccgctggcag agtgggaagc gacaaaccac ctggaacaac ttgctgacag acctctactg
     7261 ctgtggcatg gccttgacga tgacgtcgtg cctgccgacg aatcactacg tttgcagcag
     7321 gccttaagcg agacgggacg ggataaactg ctaacctgtt catggcagcc aggcgtgcgt
     7381 caccgcatta cgcctgaggc gttagatgct gccgtgacat ttttccgcca gcatctttaa
     7441 acacgcagaa tgctgacccc ttgcgcttcc agttgttgca ggatttccgg gttagcattt
     7501 ttgccggtga tcagcatatc gatttgatcg gcacggctaa aaagcattcc cgcgcgttcg
     7561 ccgatcttac tgctatcaac cagtaccacc agtttcccta ccacgcttag catcttctgc
     7621 tctgccattg ccgtcagcat atcggtttta tacaaccctt ctgctgtcag ccctttgccg
     7681 ctggtaaaca tccagtgccc ggcatagaga ctgttttcgc tgccttgcgg gcttaaagtg
     7741 atggactgac ttttgttgta ctgtccgccc ataatgatca cgctgtcatg ctcctgatcg
     7801 atcaggtaat ttgccagcgg tagataatta gtgatgattt gcactggctt gccacacatt
     7861 tcccgcccaa gcagaaacgc ggtggagccg cagttgatga ctacgctttc gcccggatta
     7921 accagctgcg aggccgcttt agcaatacgt actttttcat cgtgattctg cgcctgatgc
     7981 agattcatcg gcgtccagcg tgggcgctgc tgggtaatag cttctgcgcc attgcgcact
     8041 tttttcagtt tgccgctttc gtcaagttta ttgatatcgc gtcgcgcagt ggcaggcgaa
     8101 attcccagac gctcaacgac tttctcaacg gtcacaaagc ccaattgtgc gagcatttcc
     8161 aggaggattt gatgtctttg tgcttcagtc atgagctatt ccgataaaat ttgatttttt
     8221 tagatgatat ttgaaatagc caggaaatac tacgccggat agcgcgaact actccacgct
     8281 atccggtaca agcgacagat tacaggaacg acttgaacgg cagatccggt tcaatagtga
     8341 agcaatcatc gaaaccacgc ggatagtggt actcgaagtt gtctttatcc agcggccagg
     8401 taaatttgcc gccgacctgc cagataaacg gcttgaagcc atacttcagg cgatcttttt
     8461 tcatctccca cagcacgcgg atctcttgcg gatcggcctg gaagtttgac cagatatcgt
     8521 ggtggaacgg gatcactact ttagcattca gcgcttcgcc catacgcaag atatcagcac
     8581 tggtcatttt gtcggtaata ccgcgtgggt tttcgccgta tgaacctaaa gccacgtcga
     8641 tctgatgctc gttgccatgt tttgcatagt agttagagta gtgggaatcg ccgctgtgat
     8701 acagagaacc gcccggtgtt ttgaacagat agtttactgc gcgatcgtcc atgccgtctg
     8761 gcagcacgcc agccgctttt tgatccgctg gtagggtgat cagtgcagta cggtcgaacg
     8821 cgtcaagggc atgaatttca atgtctttca cttttacgac atcgcccggt ttgaccacga
     8881 tgcaacgctc tttcggtacg ccccagccaa tccataaatc cacgcaggtt ttcggtccga
     8941 taaacggtac gtcatcagca cagttctgca tcacggcagc agcgacgttg acgtcgatat
     9001 gatcgttgtg atcgtgagtt gccagtaccg cgtcgatctg gcgaatcgca aacggatcaa
     9061 gaacaaacgg ggtggtgcgc aggtttggct gcagtttttt cacgccagcc atgcgctgca
     9121 tctggtgacc ctgtttcatc agcgggttac cgtgactttg tttgccagta ccgcaccaga
     9181 aatcaacgca aacgttggtg ccaccttccg atttcagcca gatcccggtg cagcctagcc
     9241 accacatcgc gaatgtgcca ggggcgacct gctcttgttc aatttcttca ttcagccagc
     9301 taccccactc cgggaaagtg ctcaggatcc aggattctcg ggtgatactt ttcactttac
     9361 tcatcgccat ttaccttcat gatagttcaa ttcgaatcaa catgtgattg gttttgatta
     9421 atcctgacac tattttttca ggaaggcaat gaccattttt tgactttttg ccagggaagt
     9481 tgttgttgat tttgagtgtg gaaagattta atggaatgtg taattcaatt aactaaatga
     9541 atttaaatga ataattattt cgttgtgtga atcccactct atccatgtgg aattatttgc
     9601 gggtcgcgtc acatttaatc ataaataatc ttgttgtgat tacttttgaa aattagagtg
     9661 agtgcacaac attccgggtg tgtggaatac ccggttacct cttcttcagg agatcgttat
     9721 ggagatcctc tacaacatct ttaccgtgtt ttttaaccag gtcatgacca atgccccgtt
     9781 gttgctgggt attgtgacct gtctgggcta catcctactg cgcaaaagtg tcagcgttat
     9841 tattaaaggc acgattaaaa ccataattgg tttcatgttg ttgcaggcag gatccggcat
     9901 cctcaccagc accttcaaac cggtggtggc gaaaatgtcc gaagtctacg gcattaacgg
     9961 cgcaatttcc gatacttacg cgtcgatgat ggcaaccatc gaccgcatgg gcgatgccta
    10021 tagctgggta ggttacgcgg tgttgttagc gctggcactg aacatctgtt acgtgctgct
    10081 gcgtcgcatt accggtattc gtaccatcat gctgactggt cacatcatgt tccagcaggc
    10141 ggggctgatt gccgtcacgc tgttcatctt tggctactcc atgtggacga ccattatctg
    10201 caccgcgatt ctggtttcgc tctactgggg cattacctcc aacatgatgt acaagccgac
    10261 tcaggaagtg acggacggct gcggtttctc catcggtcac cagcaacagt ttgcatcatg
    10321 gattgcctat aaatttgcgc cgttcctcgg caaaaaagag gaaagcgttg aagacctcaa
    10381 actgccaggc tggctgaata ttttccacga caacatcgtc tccacggcga ttgtcatgac
    10441 catcttcttt ggtgccattc tgctctcctt cggtatcgac accgtgcagg cgatggcagg
    10501 caaagtgaac tggacggtgt acatcctgca aactggtttc tccttcgcgg tggcgatctt
    10561 catcatcact cagggtgtgc gcatgtttgt ggcggaactc tctgaagcgt ttaacggtat
    10621 ctcccagcgt ctgatccctg gtgcggttct ggcgattgac tgtgcggcta tttatagctt
    10681 cgcgccgaac gccgtggtct ggggctttat gtggggcacc atcggtcagc tgattgcggt
    10741 tggcatcctg gtcgcctgcg gttcctcgat cctgattatc ccaggcttta tcccgatgtt
    10801 cttctccaac gccactattg gcgtgttcgc taaccacttc ggcggctggc gtgcggcgct
    10861 gaagatttgt ctggtgatgg ggatgatcga aatctttggc tgcgtctggg cggtgaaact
    10921 taccggtatg agtgcctgga tgggtatggc ggactggtcg attctggcac cgccgatgat
    10981 gcagggcttc ttctccatcg gtatcgcctt tatggctgtc atcattgtaa ttgcactggc
    11041 ttatatgttc ttcgctggcc gcgcactgcg cgcagaagaa gatgcagaaa aacaactggc
    11101 agaacagtct gcttaataag gagttttgat tatgaccgta cgtattctgg ctgtgtgtgg
    11161 caacggacaa ggcagttcca tgatcatgaa aatgaaagtg gaccagtttt taacccagtc
    11221 gaacattgac cacacggtaa acagctgcgc ggttggcgaa tacaaaagcg agttgagcgg
    11281 cgcggatatc atcatcgctt ctacccatat tgcgggcgaa atcactgtga ccggcaacaa
    11341 atatgtggtt ggcgtgcgca atatgctctc tcctgccgac tttggcccga aattgctgga
    11401 agtgatcaaa gagcattttc cgcaggacgt gaagtaagga cgcgccatga aattacgtga
    11461 ttcgctggcg gagaataaat ccatccgcct gcaggctgaa gctgagacat ggcaggacgc
    11521 agtaaaaatc ggcgttgacc tgctggtggc ggcggatgtt gtcgagccgc gttactacca
    11581 ggcgattctg gatgcggttg aacagcacgg tccgtacttt gtgctcgcac ccggcctggc
    11641 aatgccgcac ggtcgcccgg aagagggcgt taagaaaacc ggttttgcgc tggtgacgct
    11701 gaaaaagccg ctggagttca accacgaaga taacgatccg gtggatatcc tcatcaccat
    11761 ggcggcggtc gatgccaaca ctcaccagga agtgggcatc atgcagatcg tcaatctgtt
    11821 tgaagatgaa gagaatttcg accgtttacg tgcctgccgt accgagcagg aagtactgga
    11881 tctcattgac cgcaccaacg cggcagctta agaaggaatt gaaaatgtca ttaccgatgt
    11941 tgcaggtcgc gctggacaac cagactatgg atagcgccta tgaaaccact cgcctgattg
    12001 ccgaagaagt cgacattatc gaagtgggta ccattctgtg cgtgggcgaa ggcgtgcgtg
    12061 cggttcgcga tctgaaagcg ctttacccgc acaaaatcgt actggcagac gccaaaattg
    12121 ccgatgcggg caaaatcctt tctcgtatgt gcttcgaagc taacgctgac tgggtgaccg
    12181 tgatttgctg tgcggatatc aacacagcta aaggtgcgct ggacgtggca aaagagttta
    12241 acggcgacgt gcagatcgaa ctgaccggtt actggacctg ggaacaggcg caacagtggc
    12301 gcgacgcagg cattgggcag gtggtttatc accgcagccg tgacgcacag gccgcaggtg
    12361 tggcgtgggg cgaagcggac attaccgcga tcaaacgtct ttccgatatg ggcttcaaag
    12421 tcaccgtcac cggtggtctg gcgctggaag atctgccgtt gtttaaaggc attccgattc
    12481 atgtgtttat cgctggtcgc agcattcgtg atgccgcttc tccggtggag gccgcacgtc
    12541 agttcaaacg ttccatcgct gaactgtggg gctaaggagc ggatatgttg tccaaacaaa
    12601 tcccgcttgg catctatgaa aaagcgctcc ccgccgggga gtgctggctg gaacggctgc
    12661 aactggcaaa aacgttaggc ttcgattttg tcgagatttc ggtagatgaa acggacgatc
    12721 gcctgtcgcg cctcgactgg agccgcgagc agcgtctggc gctggtgaac gcgattgttg
    12781 aaaccggcgt gcgcgtgccg tccatgtgcc tttctgctca tcgtcgtttc ccgctgggca
    12841 gcgaagatga cgcagtgcgg gcgcaggggc tggagattat gcgcaaagct atccagttcg
    12901 cccaggatgt cggtattcgc gtgatccaac tggcgggcta cgacgtttac tatcaggaag
    12961 ccaataacga aacgcgtcgt cgtttccgtg acggcctgaa agagagcgtt gagatggcaa
    13021 gccgcgcgca ggtgacgctg gcgatggaga tcatggatta cccgttgatg aactccatca
    13081 gcaaggcgct gggttacgcg cactatctta acaacccgtg gttccagctc tacccggata
    13141 tcggcaactt gtcggcgtgg gacaacgacg tgcagatgga gttgcaggcc gggatcgggc
    13201 atatcgtcgc ggtgcatgtg aaagacacca aaccaggcgt cttcaaaaac gtcccgtttg
    13261 gcgaaggtgt agtggatttc gaacgttgct tcgaaacgct caaacagagt ggttattgcg
    13321 ggccgtacct gattgagatg tggagcgaaa cggcggaaga cccggcggca gaagtggcga
    13381 aagcgcgtga ttgggtgaaa gcgcgcatgg ccaaagccgg catggtggag gcggcataat
    13441 gcaaaagcta aaacagcagg tatttgaagc caatatggat ctgccgcgct acgggctggt
    13501 gacctttacc tggggcaacg tcagcgccat tgaccgcgaa cgcgggctgg tggtgatcaa
    13561 gcccagcggc gttgcctacg aaaccatgaa agcagacgat atggtggtgg ttgatatgag
    13621 cggcaaggtg gtggaagggg agtatcgtcc gtcttccgac accgcgacgc atcttgaact
    13681 ctaccgccgt tatccgtcgc ttggcggcat tgtccatacc cattccacgc atgccaccgc
    13741 atgggcgcag gcggggctgg ctatcccggc attaggcacc acgcacgctg actacttctt
    13801 tggcgatatc ccctgtacgc gcgggttaag cgaagaagaa gtgcaggggg aatacgagct
    13861 aaacactggc aaagtgatta tcgaaacgct gggtgacgcc gagccgctgc atacgccggg
    13921 aattgtggtg tatcagcacg ggccattcgc ctgggggaaa gatgctcacg atgcggtgca
    13981 taacgcggtg gtgatggaag aagtggcgaa aatggcgtgg attgcccgcg gcattaaccc
    14041 gcaactgaat cacatcgaca gctatctcat gaataaacac ttcatgcgta agcacggtcc
    14101 caacgcctat tacgggcaga agtaaaacac gcgctgcgga gatttccttc ttcgggagat
    14161 aactggtcta agtccgcagc cgtttttcaa aaaaagcccc ctgaaaaggg ggcaaagcaa
    14221 actatggcaa tgtttcgttg gttatacctg gtgctagcga taaatatccg cgctggcgtg
    14281 catattgccg ttactccccg gttcccgcat cagaatcacg tggtagtacg ttgcgccctg
    14341 cgcatctgtc tcttcattta atgcctgacg cgcttcgctt tcagtggcga aattatgatt
    14401 gatataaatc acgcctaagc tttgcacatc gtccatattt ctggcctggt ggttattaat
    14461 ttcaatggct gcccatgtat ttgcacttag caaaagcaca gccagaaggg ctaaaacacg
    14521 gctgaacata gatacctcct cgacggctga ctttgtgtgc tctcctcttg tggtgatctt
    14581 ctgatttaat tttaatcaat gataaagaag tggatggtga ccatttctga tgcagttgtt
    14641 caaaaaaaca ccatgatgaa gtgtgatgaa cttcaaatca gcgtgttagt acgatttggt
    14701 gaaggatgag atttatttgt cggctggcct tgagtttagc ggggtatgtc agtactataa
    14761 cgcgtcattt ttcagccgac ctttaacacg ttccttgcct ccccgggatt cggctgaccc
    14821 agacaggagg ctgaataatc cgtaaggagc aattcgatgc gtcattacga aatcgttttt
    14881 atggtccatc ctgaccagag cgaacaggtt ccgggcatga tcgagcgcta cactgctgcc
    14941 atcactggtg cagaaggcaa gatccaccgt ctggaagact ggggccgccg tcagctggct
    15001 tacccgatca acaaactgca caaagcacac tacgttctga tgaacgttga agctccgcag
    15061 gaagtgatcg atgagctgga aactaccttc cgcttcaacg atgccgttat ccgcagcatg
    15121 gttatgcgta ccaagcacgc tgttaccgaa gcatctccga tggttaaagc gaaagacgag
    15181 cgccgtgagc gtcgcgatga tttcgcaaac gaaaccgctg atgatgctga agctggggat
    15241 tctgaagagt aatttctgat gaccaaccgt ctggtgttgt ccggcaccgt gtgcaggact
    15301 ccccttcgaa aggtcagtcc atcaggaatt cctcactgcc agttcgtgct tgagcatcgt
    15361 tctgtgcagg aggaagccgg ctttcaccgg caggcatggt gccaaatgcc cgttattgtt
    15421 agcggacacg aaaaccaggc cattactcac agtataacgg tcggcagtcg cataaccgtt
    15481 caggggttca tttcatgcca taaggcaaag aacggactga gcaaaatggt tttgcatgcc
    15541 gagcagattg aattgataga ttctggagac tagccatatg gcacgttatt tccgtcgtcg
    15601 caagttctgc cgtttcaccg cggaaggcgt tcaagagatc gactataaag atatcgctac
    15661 gctgaaaaac tacatcaccg aaagcggtaa gattgtccca agccgtatca ccggtacccg
    15721 tgcaaaatac cagcgtcagc tggctcgcgc tatcaaacgc gctcgctacc tgtccctgct
    15781 gccgtacact gatcgccatc agtaatcggt cacggtccat taatacgact ttgagaggat
    15841 aaggtaatgc aagttattct gcttgataaa gtagcaaacc tgggtagcct gggtgatcag
    15901 gtaaacgtta aagcgggcta tgctcgtaac ttcctggtac cgcagggtaa agctgttcca
    15961 gctaccaaga aaaacattga attcttcgaa gcacgtcgcg ctgaactgga agctaaactg
    16021 gctgaagttc tggcagcagc taatgctcgc gctgagaaaa tcaatgcact ggaaactgtt
    16081 accatcgcgt ctaaagctgg cgacgaaggt aaactgttcg gttccatcgg tactcgcgac
    16141 atcgctgacg ctgtaactgc agctggcgtt gaagtggcta agagcgaagt tcgtctgccg
    16201 aacggcgttc tgcgtaccac tggcgaacac gaagtgagct tccaggttca cagcgaagta
    16261 ttcgcgaaag tgatcgtaaa cgtagtagct gaataattcg ttattcaacg agacgtaaaa
    16321 acgccgacca gtggtcggcg ttttgctttt aagtgataaa aaccgcagaa acagacgttc
    16381 cctccataaa tctcttgcta ttccttttat aaaaagttcg cagtacccat acctggcccc
    16441 cagcctcgcc gctggtagtt cgtgtcatgt tttttacgcg taaaacacga caaaagcatc
    16501 aatacagcga tcgtctcact tgtatgataa taaaaagata aaataccatc actttcgtaa
    16561 tcttgaacgc tttgcaggtg cgttcaatcc aataaatgct aatattatgg ctatcatagc
    16621 cataaaaaga taaaatatta tcactatggg taaaaaaaca gcaccgttac tcccttatac
    16681 gaatcagatg cttagtgaat ttggtgagcg gctgaaactt gctcgtttac gacgcaggct
    16741 taccgcaaag caagttgctg aacgtgctgg gatgtcgcca ataacattgc gctctctgga
    16801 gtcaggaagt gcaggggtca cgataggggc atatttgtct gtgatgcagg ttttgggcct
    16861 ggaaacggat ttaaataagc ttgcagctga agacgactta gggcgtcagc tccaggattc
    16921 ccgtttgatt aaaaaaagag ggctttctcg tgcatcaagt aagaatgaag aaggttctca
    16981 gacagcctca ttgcagatga ggcacgagaa agaaggtcat ctcaccacgg atttaacagc
    17041 actggcagaa ggctcgacgc catctattac aggagaaaca tcaacctctc ttgcagcact
    17101 attaaaaccg ataaaaaaac cgttaaaagg aaattaatta tggcattaat cggagtgtat
    17161 gcggattggg aaggcctgga tggtccggag cggattggtt atttacacag tcgccgcact
    17221 cggacgcgtg agatatttga gtttgagtac gataaaaagg cgcttgcaga tccttcactt
    17281 aatttcattc agttagatcc tgaaataatg ttgtatgagg gagcacagta cccaattcct
    17341 ccaaaggata aatttggtgc atttagtgat tcctgcccgg atcgttgggg gcgtatgcta
    17401 atgaagcgtc gttttgagag ggatattcga gacgggttat gtgataaaga ctcccacctt
    17461 tatgagtctg attatctact aggtgttcat gatctttatc gggttggggc ccttcgatac
    17521 aaacgtgaag acgctggaga gtttcttgat aatcgcattg atgttgcagc cccaccattt
    17581 accgaaatag cgagtctgga aagggctagc cgggcaattg aagaagatcc tgataataaa
    17641 gagctaatgg ggcaaaaatg gttaagaatg ctaatcgctc cggggggatc attagggggg
    17701 gcacgtccga aagccagtgt tgttgatgaa gctgggcatc tctacatcgc taaatttccc
    17761 agtgtaaaag atgagtatga tgttggtgga tgggaaatgg ccgttaatgc acttgctgtt
    17821 ggctgtggac tgaatgttgc acctgctcag gcgcataaat ttgccagcaa ttaccattgt
    17881 tttatggttc gtcgttttga ccgcactaat gcgggaaggc gtctgcattt tgcttcggct
    17941 atgacactaa ccaggcatca ggatggtgaa gatgcctcta caggtgtgag ttatctggaa
    18001 ttagcagatg ttcttatcag gcatggcgcg cagactaata ctgatcttaa ggaactctgg
    18061 tccaggattg tttttaatat tcttgtttct aataccgatg atcatttacg caatcatgga
    18121 tttatcttga taccaggaaa aggttggcgg ctttctgagg catatgattt gaatcctgtg
    18181 gtcaggtctg atggtttaaa actcaatatt acggaaaatg acaatgcact tgatttggaa
    18241 ttagctagag aagttgcaga gtattttcga cttagtttga cggaagctga tgacattatt
    18301 gctaatttta gagggattgt cagtcagtgg cgaatcattg ccgagcgtct gagattacct
    18361 ggtcgtgagc aagagttaat ggcagaggca ttcagagcgt caatttagaa tagttatttc
    18421 gatatattcg ctaaatcata aatttgcgaa gcaatgccag tagatactga acgttttgga
    18481 agagacgttt aatttatgag aaaaatagaa acgctacaaa gctaaattat agcgtttcta
    18541 tgtaaaataa ttagcttgtc gctaactgta cttgtggttc ttcgtcttta actttgataa
    18601 gcatccacag cgcaatggcg ccaataatgt caaagaaagc cagggcaata aagaacggcg
    18661 cgaagccaat aatgtgaacg aatgccccga taaacaggtt gaaaatcaac tgaccgctcc
    18721 atgcacaggc tcctgccata cccacagcgg tagctacttc gtcttttttg aataggtcac
    18781 cgcccagagt tgctgcaaca gtagaaagac attgatgcga gaatgcgcca atgctaatca
    18841 gtaatacggc gatgtaggga ttttcgacaa tactcaccag accaatcgtc atcataatga
    18901 cggctgcaat agtgaaggta attcttcgtg agttaattaa actcacgccg cgatcgtgga
    18961 agaacttcgc gacaaaacca ctggctaaac agccgagatc gcccagcagg aaaggtagcc
    19021 agacgaacat ggcaatttct ttcaggctaa aatgcagcgt ttcgacgaag aaaatcggta
    19081 cccagaagtt aatggttccc catgccgggt cagcgagaaa acgcgcgatg ccaatccccc
    19141 agaaattacg ttggctgagg atatgcttga ttgacgtttt ttctttctta tctgttttaa
    19201 gatgctgttc ttgcccagac tcaatatagt ggcgctcttc atcagaaagg cgtttagcat
    19261 cttttggatc tttataacaa aagaaccaaa ataaagccgc gagtaaagca aggctacctg
    19321 agacaataaa cgcaaattgc cagctatgaa acataatgca ccaggcaatc aacggtggag
    19381 caagcatcgc gccgagtgag gtgcccatat tgaaaacacc tgtcgccacg ccacgctctt
    19441 ttgccgggaa ccatgttgat gcggttttta cacccgcagg aattgccgat gcttcgctga
    19501 aacccatcag ggcgcgcata aatgccagac ctgaccatgt tcctgtcaag gcatgcccca
    19561 ttgtcgccag gccccagatt gcggcacata tcgcaaaact taacttcaga cccacggtat
    19621 caataaagaa gcccattatc ggttgcccca atgtataggc caactggaat gcactgacaa
    19681 tccatgaata ttgttctttg gttattccgg ttgtctccat aatcgttgga gccgcaatac
    19741 ctaacgccgt tctgtccaga taattgatta cagtgataaa gcatacgagg gcaatcatat
    19801 gccatcgata attctttatt ttattcattt ttaaaccttt taattatatt aataaaccct
    19861 ggttgttggt atttccatgt aataggcaat aaaaatcagg tacagaggaa atattgaaaa
    19921 ttattgtgtg taaatgtctc cctttaaaaa atctggccag tttttttcaa agtaaatacc
    19981 gttaatacac ggttgcatac gtaaagactt tacttcggta ataaaagaac cttgcgaata
    20041 ttgaattgtt aatttgtgat ttttgtcttc agcgagttca gagcgaaaac gccattcttc
    20101 ttttgcgccg cgacgagttt gtggtaggca tttgccttgt ggatcaataa ccatacgcgc
    20161 cccacggcta ccaccaccag catcaatata atgcgttaat tgtgttaata ccgcggcaga
    20221 agtcagcgcc atatgccgcc acataaacag ctcggcaact tcacccacat gtttgattgc
    20281 caatccatgc cgttggacaa attcgttcaa tagcagggca tcacgagtgg cacgtcggac
    20341 tttatcggca tggcaaataa atcccgcata gtcagacatt cgtgcctgaa ttttttctct
    20401 cacaaccgac aacggcatcc cggtcccgtt atcgtgcgcc tgagtaatga tctctcttat
    20461 agaagccagt gccggagcta ccagctgtgc tatatctcca tcaatattac gtttttgcgt
    20521 gcaaccaata aaacgtgcca gacgaacagc aaaaacctgc ccggcattca atgccgcacc
    20581 accagggcga gtgacgccgt gtgtgccagc aacttccccc acggcgaaac agccgggcag
    20641 ggaggtttgt ccccagatat ccacttcaat gccgccattc atatgctgat tattcatggc
    20701 aaactgcaaa ggctgtgtgg tgagatcgta accgtgcatc ttatacagcg agatagacag
    20761 cggattcatt cgttgcagcc gttcgatggg cgatggtgcc agagcgtcgt tattttccag
    20821 atacgcgcga acgtcgtcgt ccagtcgatc taatgagaag ggcaggtcac ccggcaccgc
    20881 ttcaggattg cgattgaaat ccataaatac ctgacgccct gattgctgct cttgcgctac
    20941 agccatatct aacaagctgg agccaaactc cattacccga gtggcgtgga acggccactg
    21001 atagccttta cgaaaaatgt ttgaagccag ctcctgggtg gtgcgatagt aatccgcgag
    21061 gaagttatac tcgttgcccc cggcatccac ggaatagata tgtgggatca cctgcacata
    21121 ggtgccggac aaattccacg gaaacgtgct gcgcggtgtg cctataccaa actggctttc
    21181 agtcaaattg gttagcgtca gaccttcttc cagcgccagc cccagcgagc cgaaacattt
    21241 gtgtgggtac acactgtcgc gataaagctc gccaggccct cctgttgcca gtaccacatt
    21301 gggcgcaatc acaattgcca gcccccatgg gttatggacg cgatgaccgg tcgcgaggat
    21361 agcccccgcc acgcggtctt cgccgttttc gtcacgctga tgcagcagtt taatcactgt
    21421 tgcactggtc aacattggga tagcaagacg ctgtacttct tccagcagca ctttcaccat
    21481 taaccttgag gtacgtggcc cacacgaggt tgcacgcccg gcttcgtcat ggtcggtttg
    21541 ataacgaaga atcgcaccat agcgatcttc cggtaattcc agaccaagat attgcaaccc
    21601 gccaagagtg tgcagagaac ctaccgcttc gacataagca gtgtcgtgat ccatcgcccc
    21661 accactcgcc agtgcctctg ccagtttggt gaagttgtcg ccgttgcccg cggtagcggc
    21721 ggtaaacagc gtctgtttat cggaaccgga gcaggccgat gttcccatgt ataacccggc
    21781 ggtggcgatt aacacatttt gctggcggcg tttaagttcc acagctgcac gcagcccggc
    21841 tgcaccactg ccgactacca gggcatctgt ttgccaaagt gggtaatcaa caccgtctac
    21901 cgtgacaacc catgcgggtt ctgcctgtgc aaaacccttt gggaaagtaa cgtgcgtcag
    21961 cgcatcaagg atggtgtcat caagctgact catgcatcct ccatcggcgt aaacaggcta
    22021 ctttccatca ggcgtgggtg cttgataatt ggacggaaag ccatatacgg cagaatgtcg
    22081 cgctcaatct ccacacccgg cgcgatttca attaattcaa cgccttctgc cgttaactgg
    22141 aatactgcgc gttcagtgat atacagcact tcctgaccac tttgctgtgc gtaggcggcg
    22201 ctaaaagtga ttttttccac ttgggtaacc agtttaggga tttgacctga ctgggcgata
    22261 tgcaggccat caggcgttac atcaatcttg ctgcctttgg cgtcaaacgt accgcagaac
    22321 accactttac gggcgttttg cgcaatttcg aggaagccgc cggggccaat gagattgcca
    22381 ttgagatggg atacgttgac gttaccgtac cgatccatct ctcccatgcc gaggaaggtg
    22441 atatcgatac cgccgccact gtagaattcg aattgatcga gtgatggaat aatggcgtcg
    22501 gcgttacggg cgcagccgaa cagagcatca tccagcatca tgccgttatg tacgccttgc
    22561 tcgacgctca tccataaact ttgataaggc acgccttcgc gcagcgcaac cccaggaata
    22621 ccgcccggaa taccaaagcc aaagttggtt gaagcgcctg ccaccagttc ccgtgctgcc
    22681 cgacgggcaa tcaggcgacg caccggatga ctaaccagtt caatagcgtc gttagaactt
    22741 agacggcgat gttgaccgct gatcgtcagg tcgtaaccgc caagataggt ttgcggttgt
    22801 tcgcggtgtt caacgatacc atcgaccaga atccccggta gcttgacctt gcgaggttca
    22861 atggcgccag cttccagtac atcgcgcacc tgtacaaaca ctttgccgcc actgttgtgt
    22921 gctgccagcg ccatcgaata gctatccata tcaatcgctt cttcttcgag gctaacgttg
    22981 ccacgcggat cggcataggt gccgcgcaaa atcgcgtagt ccactttgaa agggcgatag
    23041 cggagtttgg tttcaccgtc gatttcaatc agttctacca ggtcgtcggt cgtgcattca
    23101 ttcgacttac cgccaccatt gcgtggatca acaaacgatc ccagcccaac gtgggtaaaa
    23161 agccccggac gtccggcacc gatctcccgc agcagtgcct gaattacgcc accagggaaa
    23221 cagtaggctt caatagcatt gcttttcacc agttcctgca tcttgggcga ccaggtaaaa
    23281 tgtccggcaa taatgcgttt gagcatttcg gcgtgagcaa aacggttagt gcctttgctg
    23341 tcgcgatcgc caatacccag cgagtggatt aatgtcagat cgcgcgggtg tccggtttgc
    23401 aggaaacgcg cttcaatggc ggccataata tggtcagctt ccaccatacc gccgccgtta
    23461 ccgctaatgg caatggttgc gccgtcctga atttgcgccg ccagtgtctc ggcggttgat
    23521 aacttacgca tacaatttcc ttattgattg tggaagctca cgccggcttt ttcggcaaat
    23581 gcgtgcgtcg cctgctggca atcctgagtg gcggacatta atccagccag taactggtgg
    23641 gagtaatcgg cgtgtttatt ttccagcgcc agaatcagtt gcttaatatg tgccagtgcc
    23701 accggaccac atttttccag ctgcgctaac tggttcgcca tccagctttc cacctgttct
    23761 ttttctacca cttcatcaat taaattcgcc acctgggctt cctgcgccgt caggcgttta
    23821 cccagcatca gcatctggcg accgaccacc gggccaacct gattaagcac gcgttcaatg
    23881 cccatccagc caggcaccat gccgagcatc acttccgggt tagagaattt cgcgccgggg
    23941 cgagcgatac gaatatcgca gcacaaggca agttcaatac cgccaccgat ggtatgaccg
    24001 ttgagattcg caacggttaa ttggggtaag ttgcgcaggc gattaaaaac ctcgttaccg
    24061 cgtttaatcc attttctgcc catatctaat ggcgaatagg ccgaccacga tttaatatcg
    24121 ccgcccgcac aaaagaattt atcgccggtt gccgttaatt cgactaatcg taatgtggtg
    24181 tcgttttcaa tttcatttaa ataatggtcg agagattcga tcatttcttc attaatagca
    24241 tgacatttat cttcacggtt caatgtcagg cggcaagaag ccgccgctct gctgaacaga
    24301 acgggttggt cactcatgat tattccttag caataatgtg cgatgcgatc gacgctaata
    24361 tccagcgtgc tgttaatatc tttctgccac cagtacggtg agaatatttc cagttcaata
    24421 gcgccattaa atccggcgtt ttcaaccagc cgacgaattg acggaatatt gatgacacca
    24481 tctcccggca ttccacggtc attgaccaga tcggtggtcg gcactaacca gtcggaaaca
    24541 tgaaacgcga ggatgcgttt tccggcacgc aggatctgac tggcgagatc cgggtcccac
    24601 cagacatggt agacatctac cgccacgccg aggccaaatt cgccatcggg atcgagttca
    24661 tcacaccagt ccagcgcctg acgtaacgtg cacagacaag aacgatcggc agcagtcatc
    24721 gggtgcagtg gctccagtgc aattggcacg ccgacctctt tcgaatgtgg cagcaactgg
    24781 cgaatccctt gtttcacctg ttcccgtgct tcgtacagat cttttgttcc cattggcaga
    24841 ccgccaacca cctgcatgta acaggcggca tttagcacag cggcatcgtc cagcgcccgg
    24901 cggttatcat caatcgccag cttgcgctcc gccagcgtcg gtgcggtgta gtaggtgcta
    24961 cggcacaaac cggaaacgtt tatgttactg gcagcaagct ggcgggcgat ttgctggaga
    25021 tcttcaccct gcaactccct gcgccagggg gcaatggcgc caataccgcg tgcggcgcag
    25081 gcatcaataa tggcgggtag cggcgtttta tagcccagtg tcgcggtatt gatactgaat
    25141 aaatccgggc gttgtaacag atctctcatg attcagcctc agaagcgcgg catacttaag
    25201 ccgccgtcga tggcgaccgc ctgaccacag gtgtaaatca gtttaccttc cgccatcgcg
    25261 cggacagtgg aagcaatatc agccggataa ccccaacgtc cccacggcac cagacctttg
    25321 gcaatcagtt cgtcgtaata ggccgttgcc ggaatagtca tatcggtttt gatgagacca
    25381 ggacgcactt catatacgcc aatttgttca ttacaaaggc gagcagcaaa tagacgagca
    25441 gcggcagaga cggcagtttt ggcaatggta tattcgccgc gattcatcgc cagcatgatg
    25501 gcgttgatgg aactgacgaa aataatcgag cggtgcggca gttctgcttc cggtttcggc
    25561 tgtgccagta agcgcttact gaatgcctga gcgaggaaga acggtgcgcg ggtgttaatg
    25621 gcaatatttt ggtcgaagct gtctggttcg aggtcgagaa gatcaccgcg ttttttcact
    25681 gaaatgcctg cgttgttgag caggcagtcc agacgtcccc actggttttg cgctgcgtcg
    25741 agcatctctt catgcaggga gagatcgccc acatcagcag ggaagcaaat cacttccacg
    25801 ccttccgcca gacattcttg ttgtgtggca tgtaattttt ctacgctatc cgcatccgga
    25861 cgatcattaa tcagcagatt aaatccgcca cgcgcaagct ccagcgcaca gccctttcca
    25921 ataccacgag cggctccggt aatcacggca acaggacgag tggatttcat acaatagctc
    25981 tctttttttg aattttgttt ccttagtgat gattagcttg cgactggagg gtaaaaagat
    26041 caaccatcta gttggttgat atgcgatggc gatcacagga catttgataa cctgatgaaa
    26101 gtacaagttt ttgccctttt ttctgaagct gaaatcaaat caggttatga tagtcaccac
    26161 gaagaggtcg tggggaagcg ttatgtatct tgataccagc aacgtacaga gcctgaaaga
    26221 aaagctgctg ttatgcgcgg ttaatgaatt tgccgaatat ggttacgaag gggccagagt
    26281 cgataatatc gtcaaggccg cggggtgcag taaacagacg gtttatcacc actttggtaa
    26341 taaagaaaat ttatttatcg aagtactgga atatacctgg aacgatattc gacaaaaaga
    26401 aaaggccctt gatttctcag atcttccgcc acagaaagcc attgagaaaa taatcgattt
    26461 tacatgggat tattatattt ctaatccctg gtttttaaaa attgtgcata gtgaaaatca
    26521 aagtaagggt gtgcattatg caaagtcaca gcgtctttta gaaattaacc atgcgcactt
    26581 gcagttaatg gaatccttat tggatgaagg gaagaaaaac aatattttta agccagatat
    26641 cgacccattg caggtgaata ttaatattgc tgcgcttggg ggatattatt tgatcaacca
    26701 gcatacgctt ggtctggttt atcacatcag tatggtttca ccccaggcac tggaagccag
    26761 acgtaaggtc atcaaagaga caatccttag ctggcttttg gttgaccctt catctaccgc
    26821 gcgcgaataa aactgccatc cggctggcgg gtaaacagga cctgttgatt attccccgta
    26881 tcaatggtta agcccgtcac cacgccgctg gcgttctggc ggattttcac catctgcccg
    26941 ttttgcaaat tactcagtgg cttatccgcg ccttctacct gcgccatggc atagacatcg
    27001 gtagcgggca gtccgtggtc gcggaacagc tgcgccatcg ttttaccggg ttcgacacga
    27061 taggagcgcc attgattatc aattccgcta tctggctgga atggttgcgt atgggtggtt
    27121 tgcggctgct cttctggctg gccttcctgt attggttctg gagcgaccgg agccacctga
    27181 tcaggatcat tttgtggcgt gactaattgc gcccgcagct gttcttctgt cggtggttgt
    27241 gattgcgact gaatatctaa ctgcgcttca cgcgtcacca ccggcgcgtt gggcgtatca
    27301 tcagatggaa gcagaaatcc gactaccaat acaatggcgg caataatgat cccccgacga
    27361 tgcatcggcg gcagcgggtc catgaagcga aaattgtccg gcgcgtgcca gactttctcc
    27421 agggttggtt ttagttcaaa gcgcccgggc atggtttcct cctgctccgc gtcttgttcc
    27481 tcaatcatag cctatgaata agctaacgct atgatgtccg tggtaaaccc gcctttatta
    27541 tattcatacg ggatattgct attgtttctt tttccccggg atttgtcatc attcccgcga
    27601 caaagtttta cccaaagaag tgtggctgat atgctgcccg ctactttata ccctaagaaa
    27661 ggaaatacga tgaccacccc aacttttgac accatcgaag cgcaagcaag ctacggcatt
    27721 ggtttgcagg tagggcaaca actgagtgaa tctggcctgg aagggctgct gccagaagca
    27781 ctggttgcag gcattgccga tgcgctggaa ggcaaacatc cggctgttcc ggttgatgtg
    27841 gtgcaccgcg cgctgcgtga aatccacgaa cgcgccgatg ccgttcgtcg tcagcgtttc
    27901 caggcgatgg ctgctgaagg tgtgaaatac ctggaagaaa acgccaaaaa agaaggtgta
    27961 aatagcaccg aatctggcct gcaattccgc gtgatcaacc agggcgaagg cgcaattccg
    28021 gcacgtaccg accgcgttcg tgttcattac accggaaaac tgatcgacgg caccgtgttt
    28081 gacagctccg ttgctcgcgg cgagccagct gaattcccgg ttaacggcgt gatccctggc
    28141 tggattgaag cactgactct gatgccggta ggttctaaat gggaactgac tatcccgcag
    28201 gaactggcat atggcgagcg tggcgcaggc gcgtccatcc ctccgttcag caccctggtg
    28261 tttgaagtcg aactgctgga aatcctctaa gcagcgcatt ctgttcccct cgaaccagag
    28321 gggagcagac attcagcaat aaacccttca gtttgccaaa cggcgctatt ttgtgttgca
    28381 aagaccccgt aagcgtgtat ttttgtgagc tgtttcgcgt tatcaccgtg atatgacact
    28441 cactttaaac ataaaattaa cattagatct aaatcttagt attcataccg cgtattgtta
    28501 cctaatatcg atgagtcccg atacagattc gtcgtatcat agactgacta aaggccgtag
    28561 agcctgaaca acacagacag gtacaggaag aaaaaaacat ggtagatcag gtaaaagtcg
    28621 ttgccgatga tcaggctccg gctgaacagt cgctacggcg caatctcaca aaccgacata
    28681 ttcagcttat tgccattggc ggtgccattg gtacagggtt gtttatgggg tccggcaaaa
    28741 cgattagcct tgccgggccg tcgatcattt tcgtttatat gatcatcggt tttatgctct
    28801 ttttcgtgat gcgggcaatg ggggaattgc tgctttcgaa tctggaatac aaatctttta
    28861 gtgacttcgc ttccgattta ctcgggccgt gggcaggata tttcaccggc tggacctact
    28921 ggttctgctg ggttgtaacc ggtatggcag acgtggtggc gatcacggct tatgctcagt
    28981 tctggttccc cgatctctcc gactgggtcg cctcgctggc ggtgatagtg ctgctgctga
    29041 cgcttaacct cgccaccgtg aaaatgttcg gtgagatgga gttctggttt gcgatgatca
    29101 aaatcgtcgc cattgtgtcg ctgatagtcg tcggcctggt catggtggcg atgcactttc
    29161 agtcaccgac cggtgtggaa gcgtcattcg cgcatttgtg gaatgatggc ggctggttcc
    29221 cgaaaggttt aagtggcttc tttgccggat tccagatagc ggttttcgct ttcgtgggga
    29281 ttgagctggt aggtacaaca gctgcggaaa ccaaagatcc agagaaatca ctgccacgcg
    29341 cgattaactc cattccgatc cgtatcatta tgttctacgt cttcgcgctg attgtgatta
    29401 tgtccgtgac gccgtggagt tcggtagtcc cggagaaaag cccgttcgtt gaactgtttg
    29461 tgttggtagg tttgcctgcg gctgccagcg tgatcaactt tgtggtgctg acctctgcgg
    29521 cgtcttccgc taacagcggt gtcttctcta ccagccgtat gctgtttggt ctggcccagg
    29581 aaggtgtggc accgaaagcg ttcgctaaac tctctaagcg cgcagtaccc gcgaaagggc
    29641 tgaccttctc ttgtatctgt ctgctcggcg gcgtggtgat gttgtatgtg aatcccagcg
    29701 tgattggcgc gttcacgatg attacaaccg tttccgcgat tctgtttatg tttgtctgga
    29761 cgattatcct ttgctcgtac ctggtgtatc gcaaacagcg tcctcatctg catgagaagt
    29821 cgatctacaa gatgccactc ggcaagctga tgtgctgggt atgtatggcg ttctttgtgt
    29881 ttgttctggt gttgttgaca ctggaagatg acacccgcca ggcgctgctg gttaccccgc
    29941 tgtggtttat cgcgctgggg ctgggctggc tgtttattgg taagaagcgg gctgctgaac
    30001 tgcggaaata atcgcattat catgctggat ggcgcaatgc catccagctt ttagatcact
    30061 cacccgccag cgcgcgcggg aatagtacat tgttttccag actgatgtgc tccatcaggt
    30121 catcaatcag ttcattaatg ccgttataca tcgctttcca ggtggtgcag gcttccggcg
    30181 gcggtgtgac gttattggtg gtgtgtttga tcacttccag cagttcgccc gcttcatcgt
    30241 gctcgctttc cattacgctg attggcccca ttgcctggct gcccatgcct tgtttaatca
    30301 tcgggaagag aatttgctct tctttcatca tgtggctgga aagctcttca tgcagcatag
    30361 tcaggtattt tgtcagccct tttggcacac ttggtttgtc ggcgtgaacg cgctcgactt
    30421 tagtcgcttg caggatcagc tccggcagtt gctcgcggtg gcgatcgtgg tagcgcacga
    30481 tgatatggtc gatgatttct gccagcgggg cgctacgcca gtctttctca atcggttgtt
    30541 cagcaagctt tgccagttcg gcttcaatga cgtcaacatc cagttcttta cgtgccgccg
    30601 cgcgcgccag cgtctgctta ccgccacagc agtaatccat atcatattta cgaaacagag
    30661 ccgaagcgcg aggaatagag agcgccagtt cacctaaagg ttggtcgcga taagccatag
    30721 ctgatacctc attcttaata agataagatg tattttaaat gcatctttat ggcaaatgct
    30781 ataaccctta cgtagcagag gcttatttaa ctcactgcca gcgtgtcaac tttctgagac
    30841 agattcagtt aaaggttttt ctggttttgc tctgactgca atgaccacgc caaccactaa
    30901 cagcgcgata ccgcttagcg tcattagcgg cggcatttgc tggcgtaaca aaaaggtgta
    30961 cagcaagcct gccagcgttt cgaaaacaat cagcggcccg agaatcactg tcggtaatcg
    31021 ctggctggcg acgttccagc agagtgcgcc aacccatgag caaagcacgg ctatcgcaac
    31081 catcagacta ataaacacca gcggacgggg gccaaagggt agggagaagc ccggcgtttg
    31141 cgtattcagc cagtaacagg cgacgagata gccgatgaga gaaaccggca gcgtgaccag
    31201 cgcctgcgcc gtcgcccaca tcatcggatg tttgtcggga ttctcccgca gccagcgggc
    31261 gttgcgcagg gcataccatg cccagcagac cacggaaact aacgccagca cgatgccaga
    31321 ggtataacgt gcccagtcaa aatcggggag tccgtggttt aactcagcaa tattcacaca
    31381 cgccaggccg atgccaatac aaaccagtgc cggggcgagt tttccccacg cgagtttgcc
    31441 gtcgcgctgg ctataaagca gattggcaaa aacgggaatc accaccggca gggtgccaat
    31501 aatcatcgtg gaaacaggcg cgccagtacg ttgaatggca ctggcaaggc agaagtaata
    31561 gatgaggttg cccatcatgg tgagcatcaa ggcggtaagc cagtcgcgac gcgccaactg
    31621 acgcagacgc acgcgtccca gccaggcaat gggcagcgca attaacccta acgccagata
    31681 acgccccatc gactgcaaca tcgccgggta ttccggcacg atcaacggcc cgacaaaaat
    31741 aagcccccac atcaaccctg ctaacagggc gtacagcacg ccgctaatca ttactggcat
    31801 ccattggtct gtcagaagaa agttcaggaa gcagacatga ttgctcccgg catcccgtca
    31861 ttatcagaga ggatgcggga ggatgccact ttaattaact agttattaac attaaaaaga
    31921 tggcttacgc tttcggctaa cgttgtcgtt gggcggccaa tcaatttgct aagcgttttg
    31981 ctgtcatcaa acagaccgcc tttcgatgcg ccaacgtcag aatccgccag catatccgcc
    32041 agtccatcag gcagtccgac gcttttcagt gccgcagcga aatcagcttc gctcaggttt
    32101 tgataggtaa cctgtttgcc gctctgtttg gtgagttctg ccgctaactg tgtcagcgtc
    32161 caggcactat cgcccgccag ttcgtaaacc ttgccttcgt gaccggcttc gctaatcacg
    32221 cgtgccgcag ctaccgcata atcagcccgc gttgctgagg caattttgcc atcgcctgcg
    32281 gcaccgataa atacgccgtg ctccagtgct gccggggcgc tggcgaggta gttttcggtg
    32341 taccagccgt tgcgcagcag ggtgtaaacg atgccagaat cagccagcat tttctccgtc
    32401 tcgatgtgct catcggcgag gccgagcggg gaggaatcgg catgtagcag gctggtataa
    32461 gcgataaatt tcacgccggc cgcctttgcg gcattaataa cattacgatg ctgcggggca
    32521 cgttgaccca cttcgctgga agagatcagc agtagttttt ccactccctg aagtgcagat
    32581 gtcagtgcgg cttcatcgcc gtaatcagcc tgacgcacgg taatgccttg tgctgtcagt
    32641 gcctgtgctt ttgccggatt acgaacgata gccactattt ggctggcagg aaccgttttc
    32701 atcaaggatt caataacata gtgaccaagt tggccagtgg caccagtaat agcgatcatg
    32761 ggaagtctcc atcgttttgt cttgtgttta aatacgctaa cacttaaact tacttttagt
    32821 aagtacgtac aaaaaggtaa gtatgagatg agtcaggtta gcctgtcgca acaactgaaa
    32881 gagggaaatc tcttcgctga acagtgtccg tcgcgcgagg ttttgaaaca cgtcaccagc
    32941 cgttgggggg tgttgattct ggtggcgcta cgcgaaggta ctcatcgctt tagcgacctg
    33001 cggcgcaaaa tgggcggggt gagtgaaaag atgcttgcgc agtcgttaca ggcattagaa
    33061 caggatggtt ttcttaaccg tatcgcgtat ccggtggtgc cgccgcatgt ggaatatagc
    33121 ctcacgccgc tgggcgagca ggtgagcgaa aaggttgctg cactggcgga ctggattgag
    33181 ttgaatttgc ccgaggtgtt agcggtgcgg gatgaacgtg cggcataact gccggatgcg
    33241 ctgcgcttat ccggactacg gttcatggct tgtagggcgg ataagatgcg tcatcatcgc
    33301 atccgccaat aaatgccgga tgcggttgat gatttgtagg ccgaaaaaga agtgttttac
    33361 ttactcagat ccacctgata aatcgcaaac ccgatatcat cggtcgcgac tttattcatc
    33421 ggatactgcc ctttctcttt aataaacact gcggctttat ctgacggaga ggtttcgaaa
    33481 cggatatcca gtttcttatc gccagctatt ggtgctaaac gccagttgtt atctgccgcc
    33541 gggtgaattt cccccgcacg tttcgactca tcagcaatcc acgctgccag caccgagcgg
    33601 ttctcatccg gtgaagcaaa agcgatatgg ctgtcgcccg taccggcaaa tttgccgccg
    33661 taagcgcgat agttattggt ggcaacgagg aacatggcgt tcggatcaat cggcttgccg
    33721 ttaaaggtca ggttcttaat cctttccgca ttggcattaa tcatctggca ctcgccgtca
    33781 taacgggcgg gctgggtaac atcaatctga taattcacac catcaataac atcaaagtta
    33841 taggtgcgga aaccatccca gttgatgagt gactgtggtt tcgtgctgtt aggatcaatc
    33901 tggttaaact gtccggcgga gcactccagc cactctttca cctctttacc gctggctttc
    33961 accacaatca gcgtattggg gtagagataa agatcggcgg cattacggaa ggtcaactgg
    34021 cctttttcca cctccacata gcttgccggg tcatttttgc gaccaccaac tttaaacggt
    34081 gcggcagctg aaagcactgg cagttttgcc agatccggat cgccctgaat gtaatgctcg
    34141 acatacgctt tttgcgcgtt gttcaccacc tgcacggtcg gatcgtcctg cacaagcgcc
    34201 agatagctat acatattgtc ggcagattta ccgattggct tgctgacgaa ctggcgtgtg
    34261 gcatcgtgat cggctttgag tgtttctacc agcttgctgt cttccgccgc gagggatttt
    34321 ttattcgcga tgtcgtaaat cggccgtgct tccgctttcg cctgcgtcac ctgccattta
    34381 ccgctgtcat tactgagttg taagtcgacc acaccaagat gatcgcccca catgcctggc
    34441 attaccgccg gaacaccatt cagcgtgcct ttcgcgatat cagccccttc gatatcagca
    34501 aaatctttac ctgggaaaac ggcgtgagca tggccaaaca taatggcgtt aacgcccgga
    34561 atttcactga ggtaataaac tgagttttcc gccatcactt tatacggatc ggcagacagc
    34621 ccggaatgcg ccagaacgac aacgacatcg gcacctttct cgcgcatttc aggcacgtat
    34681 ttgcgcacgg tttcggtaat atcattcacc gtcactttcc cggataaatt agctttatcc
    34741 cagcccatga tttgtggcgg gacgacgcca atataaccaa tcttcagcgt ctgttttttt
    34801 ccgtctttat cgaccacttc ggtgtcttta attaaatacg gtgtaaacat tggctgtttg
    34861 gttctggcgt caatgacgtt ggcatttaca taagggaatt tcgctcccgc cagcgcattt
    34921 ttcagataat ccagaccgta gttaaactcg tggttgccaa gcgtgccgac ggtatagtcc
    34981 agcgtattta atgccttata gaccgggtga atatcacctg cttttaaacc tttcgccgac
    35041 atgtaatctg caagcggact cccctgaatc aaatcgccgt tatcgaccag tacgctgttt
    35101 ttcacttcat tgcgggcatc gttaatcagg cttgcggtac gtaccagtcc gaatttttcc
    35161 gtggcggtgt ctttgtaata atcgaaatcc atcatgttgc tatgcagatc agtggtttcc
    35221 atgatacgca gatcgaccgt cgctgcattc acactggcgg caatcagcgt ggccaggagc
    35281 gttgcgctaa acttaatcat cagggacatc cttttatcat cgggaatacg aaagaaaagg
    35341 gagaataaac gtcttactta tagaacagtg aagaatgcca caattttacg ctttgaaaat
    35401 gatgacacta tcacagttgg cgcattcatt aacgataggg tataagtaaa acaataagtt
    35461 aacaccgctc acagagacga ggtggagaaa tgttagatca agtatgccag cttgcacgga
    35521 atgcaggcga tgccattatg caggtctacg acgggacgaa accgatggac gtcgtcagca
    35581 aagcggacaa ttctccggta acggcagcgg atattgccgc acacaccgtt atcatggacg
    35641 gtttacgtac gctgacaccg gatattccgg tgctttctga agaagatcct cccggttggg
    35701 aagtccgcca gcactggcag cgttactggc tggtagaccc actggatggt actaaagagt
    35761 ttattaaacg taatggcgaa ttcaccgtta acattgcgct catcgaccac ggcaaaccga
    35821 ttttaggtgt ggtgtatgcg ccggtaatga acgtaatgta cagcgcggca gaaggcaaag
    35881 cgtggaaaga agagtgcggc gtgcgcaagc agattcaggt ccgtgatgcg cgcccgccac
    35941 tggtcgttat tagccgatcc catgcagatg cagagctgaa agagtatctg caacagcttg
    36001 gcgaacatca gaccacgtcc atcggctctt cgctgaaatt ctgcctggtg gcggaagggc
    36061 aggcgcagct gtacccgcgc ttcggaccaa cgaatatttg ggacaccgcc gctggtcatg
    36121 ccgtggctgt agctgccgga gcgcacgttc acgactggca gggtaaaccg ctggattaca
    36181 ctccgcgcga gtcgttcctg aatccggggt tcagagtatc tatttattaa ttaatttatg
    36241 cagcaggtcc atcacttgct gcacctcttc ctgagtaagc gccccgtctt tggcccattg
    36301 cacgcgaccg tctttatcca gcaccactac agcggaactt tcttcatcca gctgccaggc
    36361 accgcgcgcg acgccattgc tatcgacaat aaactgcgac cagggataaa gctttttatt
    36421 actctccaga ctgctgcgca caaacatgcc ggaacccgga attgcgtcgt cggtgttaac
    36481 aatggtggtg gtctggtaac gatcgtgcgg taactttgct gatttaatcg cttcaatcag
    36541 cgtcgcgttt ttctcttttg cagaggtgcg accagcaata tgttgcagta ctcgcacttt
    36601 tcccactagc tgcgcgctgt tccaggtttt gtagctaaac tgatctttat caagcaccaa
    36661 ctcgccacga tcggtaatgc caatcggcgg cactcgctga ccggtttcga actgatgtgc
    36721 ggaagccatc atcggcaaca gcaggcaggt gagtgccaga atcttgcgta gggtcatggt
    36781 gtttccttct tatgatatgc aggtgatccg accacttggg tctgagtttt aatcatatgt
    36841 gctatttatc gaatttcccg caagtgtgat gccagtttgc ggtcaagcgc acaaatcata
    36901 tgaaaaatga atgcttatac tgaagacggc gcttcggtaa aaagataatt ctgaataatt
    36961 gtaaccttta ggtaaaaaaa gttatacgcg gtggaaacat tgcccggata gtctatagtc
    37021 actaagcatt aaaatttgcg cctcataata gttgggccga ttgtggcacc gcacaggcgt
    37081 aatactcagc aggagataac aatgaaaatt ttccaacggt acaacccact tcaggtggcg
    37141 aagtacgtaa agatcctgtt ccgtggacgg ttatacatca aggacgttgg cgcttttgaa
    37201 tttgataagg gtaagattct tatcccaaaa gtgaaggata agctgcattt gtcagtgatg
    37261 tccgaagtta accgtcaggt tatgcgtctg caaacagaga tggcttaacc aaagtgctat
    37321 gcagtaataa gacggctcct gattcaggag ccgttgatgt ttctgggggt tacgcgacgc
    37381 tttcttcttt atctttagcg tcaggcaatt ttggcgaaag ggcggtggcc ttgctgtcga
    37441 ttcgggtcac cagcagctgg tcgatgcggt agttatcgat atccaccact tcaaatttgt
    37501 agccggcgaa tttcaccgaa tcggtgcgtt tcgggatctt acgcagcata aacatcataa
    37561 agccgccgat ggtttcgtag ttgcccgact gcgggaactc gtcaatatcc agcacgcgca
    37621 tgacgtcgtc aataggcgtg ccgccgtcta tcagccatga gttctcatca cgggcgacaa
    37681 tttgctcttc cagcccctga ccgaccagat cgcccatcag cgtggtcatc acgtcgttga
    37741 gggtgatgat ccccactacc agcgcgtact cgttcatgat caccgcgaag tcttcacctg
    37801 cggttttaaa actttccaac gcctctgaaa gggttaacgt atccggcaca atcagcgtat
    37861 tgcgaatttg tacgccgctg ttcagtgcca ggctttggtt agccagcacg cggttcagca
    37921 ggtctttaga atcgacatag ccgatgatgt ggtcaatatc ttcattacag acgagaaact
    37981 tagagtgcgg atgttccgcc accttattct tcagactttg ctcatcttcg tggagatcaa
    38041 accaaatcac gttttcacgc ggtgtcattg aagacggaac ggtacgggat tccagctcaa
    38101 agacgttttc aatcagctcg tgttcctgtt tacgtaacac gcccgccagc gcaccggctt
    38161 ccactaccgc gtagatgtca tcagaagtga tatcatcttt acgtaccatt ggcagtttga
    38221 aaatacggaa gattatgttc gccagtccgt tgaagaacca caccagcggg gtgcaaacgt
    38281 acaggcagaa gcgcatcggg ttgatgatac gcaaagccac agcttctggc gcaatcatac
    38341 cgatgcgttt cggggttaaa tccgcaaaca ggataaacat gccagtcact aacgagaaag
    38401 agagaataaa gctcagttgc tcagagagtt ctgccgacat atagcgggag aacaggctgt
    38461 gaaaagctgg agaaaatgcc gcatcaccga cgataccgcc gagaatcgcc actgcgttca
    38521 gaccgatctg gaccacggta aagaacatgc cgggattttc ctgcatattc agaacgcgtt
    38581 gggcatttat attgccttca tcagccagca gtttaagttt gattttgcgt gaggcggcaa
    38641 gcgagatctc ggacatcgag aagaacgcac ttacagcgat caaacagagt atgactaaaa
    38701 tactgtttaa catatcttat ccgacctttc aggccagatc ctcggaaggg gaagttgatt
    38761 aatttgtgtg aaatacacat tgaaagccga ttcgaagaaa gtgaaccggc attttcagtg
    38821 gctagtatag cgtaaggtac tgtaaagccg ccagagggtt aaaattcaga cagctgaaaa
    38881 atgaaaaact gcctgatgcg ctatgccgaa caggcctacg cagtccccgc aatatattga
    38941 atttggacga ttttgtaggc ctggcaaggc gtttacgccg tatccggcat aaacaaaatg
    39001 cactttatca acaatctaca tttgtacccg taacgctatg cttccggcgg cagacagacg
    39061 ccaattccgc caattccaca gtaaccatac gggtttttat gcagatattg ctggtggtca
    39121 tcttcggcat aataaaacgg tgtggcgtta gcgatttccg tggtgatgcg acgatcgtca
    39181 tcggcggcaa gcatcgccgc ctgaaaacgt tccagactgg cgcgagcggc ggcatcctgt
    39241 tctggggtca gcggataaat cgctgaacga tactgcgtgc cgtggtcatt gccctgacgc
    39301 attccctggg cgggatcgtg attctcccaa aatacttgta gcaactgctc atagctgatg
    39361 acggatggat cgtaaacaat gcgcaccgct tcggcatggc cagtatcgcc ggagcacact
    39421 tcccgataag tcggatttgg cgtatagccg ccggtatagc ctgcggcggt gctgtaaacg
    39481 ccgggtaact gccagaacag acgctccaca ccccagaaac aacccatcgc aaaaatggca
    39541 atctccattc cgtcaggtac attggtcatt gagtgaccgt tgaccgcatg cagtgtggct
    39601 acgggcatcg gggtgttacg tccaggcagg gcatcggcgg gggaaaccag atgcttttta
    39661 tcaaataaac tcatggtgtc gctctcccga aaatcggtca ttggggttaa ggttgtaaca
    39721 agaggcgtat ttgcacacaa taaccaccgt gaataggtct aaagtaaaac ataagaaata
    39781 ttttgggttt agtctgcttt ttaatccata ttactggatt tttgttaagc cgtttaacgg
    39841 cgttccaggg gcaggaaaaa aggatattca ggagaaaatg tgcgctatat ccgacagtta
    39901 tgctgtgtaa gcttactctg cttaagcgga tctgccgtcg ccgcgaacgt ccgtctacag
    39961 gtcgaggggt tatcgggaca gctggaaaag aacgttcgcg cgcagctttc tacgattgaa
    40021 agtgatgaag tgacgccaga ccgtcgcttt cgcgcacgcg tcgatgatgc catccgcgaa
    40081 ggtctgaaag cgctgggcta ttaccagccg accattgaat ttgatctccg tccaccgcca
    40141 aagaaagggc ggcaggtatt gatcgccaaa gtcacgccag gcgtgccggt gttaattggc
    40201 ggcaccgatg tggtattgcg cggcggcgcg cggaccgata aagactattt gaaattgctc
    40261 gatactcgcc cggctattgg tacggtactg aaccagggcg attatgaaaa tttcaaaaag
    40321 tccttaacca gcattgcgtt gcgtaaaggt tatttcgata gcgaatttac caaagcgcag
    40381 ctgggcattg cgctcggcct gcataaagcc ttctgggata ttgattataa cagtggcgaa
    40441 cgttaccgct ttgggcatgt gacctttgaa ggatcacaaa tccgcgatga atacctgcaa
    40501 aatctggtgc cgtttaaaga gggcgatgag tacgaatcga aagatctggc agaactgaac
    40561 cgtcgacttt ctgctaccgg ctggtttaac tcggtggtgg tggctccaca atttgataaa
    40621 gcgcgcgaaa cgaaagtatt accattgacg ggcgtggttt cgccgcgaac agaaaacacc
    40681 atcgaaaccg gcgtcggtta ctctacggac gtgggaccgc gcgtgaaagc gacgtggaaa
    40741 aagccgtgga tgaactctta cggtcacagt ttgaccacca gtaccagtat ttccgcgccg
    40801 gaacagatcc tcgacttcag ctataaaatg ccgctgctga agaatccgct ggaacaatat
    40861 tatttggtgc agggcggttt taagcgcact gacctgaacg ataccgagtc tgactccact
    40921 acgctggtgg cttctcgcta ctgggatctc tccagcggct ggcagcgtgc cattaacctg
    40981 cgctggagtt tcgaccactt tacccagggt gaaattacca acaccacgat gctgttttat
    41041 cctggggtga tgattagccg cacgcgttct cgtggtggcc tgatgccaac ctggggcgac
    41101 tcgcaacgct actctatcga ctactccaac acggcctggg gctcggatgt tgatttctcc
    41161 gttttccagg cgcagaacgt ctggatccgc acactgtacg atcgccatcg ttttgtgaca
    41221 cgcggcacgc tgggctggat tgaaacgggt gatttcgaca aagtaccgcc ggatctgcgt
    41281 ttcttcgccg ggggcgatcg cagtattcgc ggctacaaat acaaatctat cgccccgaaa
    41341 tacgccaacg gtgacctgaa aggggcctcg aagctgataa ccggatcgct ggaataccag
    41401 tacaacgtga ccggaaaatg gtggggcgcg gtgtttgtcg atagcggcga agcggtaagc
    41461 gatattcgcc gcagcgactt taaaaccggt accggggtcg gcgtgcgctg ggaatcgccg
    41521 gtcgggccaa tcaaactcga ttttgccgta ccggtcgcgg ataaagacga acacgggtta
    41581 cagttttaca tcggtctggg gccagaatta tgagtttatg gaaaaaaatc agcctcggcg
    41641 tggttatcgt tatcttactg ttgctgggat cggtggcgtt tctggtgggc accaccagcg
    41701 gcctgcatct ggtatttaaa gcggcggatc gctgggtgcc aggactggat attggcaagg
    41761 tcaccggcgg ctggcgtgat ctcaccttgt ctgacgttcg ttatgagcag ccaggcgtgg
    41821 cggtaaaagc gggcaatctg catctggcgg tcgggcttga gtgcctgtgg aacagtagcg
    41881 tttgtattaa tgatctggcg ctgaaagaca ttcaggtcaa catcgacagt aaaaaaatgc
    41941 ctccttctga acaggttgaa gaagaggaag atagcggtcc gttggatctc tccatgccgt
    42001 atcccattac cctgacacgg gtggcgctgg acaacgtcaa catcaagatt gatgacacca
    42061 cggtatcggt gatggacttc acctccggcc tgaactggca ggagaaaacc ctgaccctga
    42121 aaccgacatc gctgaaaggc ctgttgattg ctttgccgaa agtggcggaa gcggcgcagg
    42181 aagaagtggt cgaaccgaaa attgaaaatc cacagccgga ggaaaagccg ctcggcgaaa
    42241 cgctgaaaga tctcttttct cgcccggtat tgccggaaat gaccgacgtg catctgccgc
    42301 tcaacctgaa cattgaagag tttaagggcg agcaactgcg cgtgacgggc gacacggaca
    42361 tcaccgtgcg caccatgctg ctgaaagtga gcagtatcga cggcaatacc aaactggacg
    42421 cgctggatat cgactccaac caggggatcg tcaacgccag cggcacggcg cagctgtcag
    42481 acaactggcc ggtggatatc accctcaaca gcacactgaa cgtggagccg ttgaaaggtg
    42541 aaaaggtgaa gctgaaagtg ggcggtgcgc tgcgcgaaca gctggagata ggcgttaacc
    42601 tttccggtcc ggtggatatg gatttacgcg cccagacgcg actggcggaa gccggattgc
    42661 cgctcaacgt ggaagtgaac agcaaacagc tttactggcc gttcactggt gagaagcagt
    42721 atcaggcgga tgatctgaaa ctgaaactta ccggcaaaat gaccgattat gcgatctcta
    42781 tgcgcacggc tgttaagggc caggagatcc cgccagccac cattaccctt gatgccaaag
    42841 gtaatgaaca gcaggtcaat ctcgacaaac ttaccgtcgc ggcgctggaa gggaaaacgg
    42901 aacttaaggc gttgctcgac tggcagcagg ccattagttg gcgcggtgag ctaacgctta
    42961 acggtattaa caccgccaaa gagatcccgg agtggccgtc gaaactcaat ggcttgatta
    43021 aaacccgcgg tagcctgtac ggcggcacct ggcagatgga ggtgccagaa ctgaagctga
    43081 ccggtaacgt caaacagaac aaagtgaacg ttgacggcac gctgaaaggc aacagttata
    43141 tgcagtggat gatcccaggg ctgcatctgg aactggggcc aaacagtgcc gaagtgaaag
    43201 gcgagctggg ggtaaaagat ctcaatcttg atgccaccat caacgcgccg gggctggata
    43261 acgcgctgcc ggggcttggc ggtacagcga aagggttggt gaaagtacgc ggcacggtgg
    43321 aagcgccaca actactggca gatatcaccg cgcgcggcct gcgctggcag gaactttccg
    43381 tggcgcaggt tcgcgtggaa ggcgatatca aatccaccga ccagattgcg ggtaaactcg
    43441 acgtacgcgt tgagcaaatt tcgcagccgg atgtaaatat caacctcgtc accctgaatg
    43501 ccaaaggcag cgaaaagcag cacgagctac agttgcggat tcagggcgag cctgtctccg
    43561 ggcagcttaa tctggcagga agttttgatc gcaaagaaga acgctggaag ggaactctta
    43621 gcaatacccg cttccagacg ccggtcggcc cgtggtcgct gacccgcgat attgcgctgg
    43681 attaccgcaa taaggagcaa aaaatcagca tcgggccaca ctgctggctt aacccgaatg
    43741 cggaactgtg cgtgccgcaa actatcgatg cgggtgccga agggcgtgcg gtggtgaatc
    43801 tcaaccgctt cgacctggcc atgctgaaac catttatgcc agaaaccact caggccagcg
    43861 gtatcttcac gggtaaagcg gacgttgcct gggacaccac gaaagagggg ctgccgcagg
    43921 gcagtatcac cctttcgggg cgtaatgtgc aggtgacgca aaccgtcaac gatgcggcgc
    43981 tgcccgtggc gtttcagacg ctgaatctga cggcggaatt gcgtaacaac cgtgccgaat
    44041 tgggctggac catccgcctg accaataacg gccagtttga tggacaggtg caggtgaccg
    44101 atccgcaagg ccgccgtaat cttggtggca acgtcaatat ccgtaacttc aaccttgcga
    44161 tgataaaccc catctttacc cgtggggaaa aagcagcggg gatggtgagt gccaacttgc
    44221 gtctgggtgg tgatgtgcaa agcccgcagt tgtttggtca gcttcaggtt acgggtgtgg
    44281 atatcgacgg caactttatg ccgtttgata tgcaaccgag ccagcttgcg gtcaacttta
    44341 acggtatgcg ctcgacgctt gccggtacag tacggaccca gcagggtgaa atctacctga
    44401 acggtgatgc cgactggagc cagattgaaa actggcgggc gcgggtaacg gcgaaaggca
    44461 gtaaagtgcg gatcaccgtg ccgccgatgg tacgaatgga tgtatcgcca gatgttgtat
    44521 tcgaggctac accaaacctg tttaccctcg atggtcgcgt ggatgtcccg tgggcgcgca
    44581 tcgtggtgca tgagctgccg gaaagcgcgg tgggcgtctc cagcgatgtg gtaatgctta
    44641 acgataacct gcaaccggaa gaggcaaaaa cggcgtcgat tccgattaac agcaacctga
    44701 ttgtccacgt cggcaacaat gtgcgcattg acgcctttgg cctgaaagcg cggctgacgg
    44761 gcgatctcaa cgtcgttcag gacaaacaag ggctgggcct gaacgggcag atcaacatcc
    44821 ctgaagggcg cttccatgcc tatggtcagg atctgattgt gcgtaagggt gagttactgt
    44881 tctctggtcc gccggatcaa ccgtatctta atatcgaagc tattcgtaac ccggatgcta
    44941 cagaagacga cgtaatcgcc ggagttcgcg tcactggtct ggcggacgaa ccgaaagcgg
    45001 agatcttctc tgacccggcc atgtcgcaac aggctgcctt gtcttatttg ctacgtggac
    45061 aagggctgga gagcgatcag agcgacagtg cggcgatgac ctcgatgctg attggtctgg
    45121 gggttgcaca aagtggtcag attgtgggta aaatcggcga gacgtttggc gtaagcaatt
    45181 tagcgctcga cacccaggga gtaggcgact cctcccaggt ggtggtcagc ggctatgtat
    45241 tgccaggtct gcaagtgaaa tacggcgtgg gtatatttga ctctatagca acactcacgt
    45301 tacgttatcg cctgatgcct aagctatatc tggaagccgt gtctggtgta gaccaggcac
    45361 tggatttgct ctatcagttc gagttttagc aatgcgaata tttgtctacg gcagtttacg
    45421 ccacaaacaa ggcaacagtc actggatgac caatgcccag ttactgggcg atttcagtat
    45481 cgataactac cagttgtata gcctgggcca ctatccaggc gcagttccgg ggaacggaac
    45541 agtacacggt gaagtttatc gtattgataa cgccacgctg gccgaacttg atgccttgcg
    45601 caccaggggc ggtgaatacg cgcgccagtt gattcagacg ccgtacggga gtgcatggat
    45661 gtacgtttat caacgacccg tcgatggatt aaagctaatt gaaagcggcg actggttaga
    45721 cagggataag taataataaa agcacgccac cttcgggtgg cataaggggg tagcgcgtat
    45781 cgctatcccc ctttatatat caaatggtta cgagttcaaa gtggttcgga atagtgccat
    45841 ttagtccccg tttatgggca aaatctggac aactccgcaa ccggattgaa ccttaccgcg
    45901 tcctggagat aatcaggtgc aaagtgcgca taggtcattg tctgctgaat tgtgctgtgc
    45961 cctaaaatac gctgaagggt aataatgttc cctccattaa tcatgaaatg agttgcgaat
    46021 gtgtgccgta aaacatgcac agactgaccg actggtaaat caggccgcac agtccttatt
    46081 gcacttctga ctatcgcata actggggttc attaatcttc ccgtcgcttt gactttaatc
    46141 agagattcaa gagctttagc tataggaata acccgccgcg atccattctt tgtctccatg
    46201 aaaataacct tcccaccaat tatgtgttca cctttaatat tggcaacttc accccagcgt
    46261 ccgcctgtag ccaggcttaa caatacagcc ttaagattat cgccagataa aagtgcggtt
    46321 agcctgggaa tatcatcatg tgcaagataa gccatttccg gctgattaat tgttagcttg
    46381 cgtatctcgt gaaacgggtt atcacccaca aactcttcaa catcaatcag cttttcaaag
    46441 aaaccgctca tcattgcaca ataacgattt accgttgact gcttaattcc gcgattcatt
    46501 aattcaaggc gatatctgat aatatttttt cgggttaagt ggtcacttct gttaatacca
    46561 agctcttgca tatcacgaag aaccgcttca attctgaccc gattcatcgc gccataacga
    46621 tgattcttac cgtggtacac ccaccacagt tccagaagat cgcttaacgc tttacgttca
    46681 ataggttttt cgacccagtc cttatcgtga taatgcgtca gaacatggcg ttcatacatc
    46741 gccgcttcac cacgggtagg aaatcgccgc cgaaatcttc ttccttcaga tccagcaggc
    46801 cgcacgtcca cacagtattt gccatcagcg agcttcttaa tcgacataag gaagcccccc
    46861 aatgaaaaca tcatcttgat cacaattttg agaaataaag gccagatgga ccgttaacca
    46921 gtcttcttgc cggagtggga tgattccgct tcgctttgcc caatgtgtgc gagagccggt
    46981 accacctgcc cggcctttgg ttcgacctca tcgaacaaaa accagtcacg gtatttacgg
    47041 aaacgagcgt gccgcaaaag aaggatccca aaatcaagag gcatcttaga tttcccactt
    47101 tcatagccat gatatgtata gtaattaatt ccaattaaat cagatagttc cttcattttc
    47161 atccgttcgg actctctgat aagttttaat ttctcatttt gcggtattga cataattttt
    47221 ctaatctcca ataatttttg tgtatagcaa cacaagaata gtcgcaattg gttctacatg
    47281 gtccctattc gaaaaagggg ccaataacgg aggataccag atgcaagaag tcactcaaaa
    47341 cgcgcctgaa tcgcctgccg aaacacagtg cgatcagttg acagatgcgc cagaaaccgc
    47401 acaccaaaag accgggcgca aagcccctga acgccaggaa atcaggcttt cacaaaaccc
    47461 ttctcatctc ctgtcaaaag aagggtttgc gatgtatatc ggcaagaccg ttgacgccat
    47521 cgtgagcatg gcaaaagcag ggaaattacc cgctgtgtac atggcggatc ccctcaagcc
    47581 tggcggaaac gccgaacttt acatcaaccg gaaagcatgg gatgaagcct gcgatcgact
    47641 cactgaaaac gcaccgcaag aatggcacga atgggaaaac cgactttttt tatttaagcc
    47701 agcagctggt tcacggaata aacgcatcag taaaaaagct gcgtgatata gaactggcaa
    47761 tcattgggag gacaggatga ttaagaaata cagcgaacac gggagcaagg ctggcgaagc
    47821 aatcgccatt gacgaggatt gcctgataac aggagacccc tggctggcaa cagaaaggga
    47881 tttagaaggc aaaacatttt ttgtcagagc aataaagcac aaagataggg ttgtgggtgg
    47941 aattatttat tcaaccagta cgattattcc tgataattat ctggatattt cttttagacc
    48001 tgtaagcatt caggggtgcg aggcatacct taattacgtt gcggagctaa agaaaattaa
    48061 cctggactac atgtggccaa tcgtacaacg cgaaataatg cgacgaaaat cagaaggctt
    48121 cgatgtgaaa attgagtttt tatcgacgtt aatgggattt ttacaaaaac cattgattct
    48181 tttcccctcc cttgaccgga acctgccagt caaagaaggg gtggaaaaat atattgttga
    48241 acaagtcaga gatctggttt gtctttagag gcgcgaataa agcacgcaag atgattaata
    48301 ctatgaaatc gtgtccttat attcacggca tagggagcaa aggcttgttg cccctctatc
    48361 tgacgcaaaa tttctgacgc agttttcatc aatgcatccc ttttatcatc gggaagttcg
    48421 ctaatgagga gcgaaataag cagatcggac accactccca ctgagtctct tgggtcagtc
    48481 aacttatcaa caggagccac agcaagatta gttgtgtaaa tgctaaccag gtattcagtg
    48541 atagcgtcct taacgctttt atcaatcatg ttagttacct ttttctggtt gtgtggaaac
    48601 accagaatac cacgcgccgg gcgtggttaa acatcccggc actgattttc atttaactta
    48661 aacggggttt tccgatatga cacagagacg cagcttccgc actggcacag aacgccacgc
    48721 agcccgtttc tcaaccagcg catcacgcag caactcgcac tacagcctgc aggaaacgca
    48781 cgccacacct gacgggtatc tggttaaaca aacaggtaaa cacacctggc tgattaatga
    48841 tgccggaatt gtcattcacc ggggcgcacg caacccgatt accggaaata cctgttactc
    48901 cctgactcgc ggagatgagc agttcgggca ggatttcacc ctgcacgaag cattaagaac
    48961 ggcagaccgg ttaatcagcg gtcgcagttt tcagcacccg gaacatcgcc actgataaat
    49021 aaaaggattc agccatgatc gaagcctaca aaatacttat taataatgcg ttgcagcgtt
    49081 atcacttcga actcgcaaac cggggtgtaa cccgggacga aggacttcgt aactcactgc
    49141 ggggcgcaat tatggggctg tataactgcg cactgggcag cgatgaccgc gaagcactgg
    49201 aagaattaaa agttattgtg gccgaactgt ttgaaggtga aatcgt
//
LOCUS       DAZGQL010000023        49091 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.023, whole genome shotgun sequence.
ACCESSION   DAZGQL010000023 DAZGQL010000000
VERSION     DAZGQL010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 49091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49091
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.023"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            434..523
                     /gene="tisB"
                     /locus_tag="ACHKIR_001585"
     CDS             434..523
                     /gene="tisB"
                     /locus_tag="ACHKIR_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HFO9989337.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            complement(647..721)
                     /gene="ysdE"
                     /locus_tag="ACHKIR_001586"
     CDS             complement(647..721)
                     /gene="ysdE"
                     /locus_tag="ACHKIR_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsdE"
                     /protein_id="HFO9989338.1"
                     /translation="MNVSQIYARNGELFSGRICKQKRQ"
     gene            803..1987
                     /gene="emrD"
                     /locus_tag="ACHKIR_001587"
     CDS             803..1987
                     /gene="emrD"
                     /locus_tag="ACHKIR_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HFO9989339.1"
                     /translation="MKRHRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLIPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(1995..2492)
                     /gene="yidF"
                     /locus_tag="ACHKIR_001588"
     CDS             complement(1995..2492)
                     /gene="yidF"
                     /locus_tag="ACHKIR_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFO9989340.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQLGIRSVQLI
                     PHLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDTTLQKWCGSEKS
                     QPRRDCQACDWHRLCPHAREDKPDNVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(2489..2851)
                     /gene="yidG"
                     /locus_tag="ACHKIR_001589"
     CDS             complement(2489..2851)
                     /gene="yidG"
                     /locus_tag="ACHKIR_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HFO9989341.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(2841..3188)
                     /gene="yidH"
                     /locus_tag="ACHKIR_001590"
     CDS             complement(2841..3188)
                     /gene="yidH"
                     /locus_tag="ACHKIR_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HFO9989342.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            complement(3248..4741)
                     /gene="yidJ"
                     /locus_tag="ACHKIR_001591"
     CDS             complement(3248..4741)
                     /gene="yidJ"
                     /locus_tag="ACHKIR_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO9989343.1"
                     /translation="MKRPNFLFIMTDTQATNMVGCYSGKPLNTQNIDSLAAESIRFNS
                     AYTCSPVCTPARAGLFTGIYANQSGPWTNNVAPGKNISTMGRYFKDAGYHTCYIGKWH
                     LDGHDYFGTGECPPEWDADYWFDGANYLSELTEKEISLWRNGLNSVEDLQANHIDETF
                     TWAHRISNRAVDFLQQPARADEPFLMVVSYDEPHHPFTCPVEYLEKYADFYYELGEKS
                     QDDLANKPEHHRLWAQAMPSPVGDDGLYHHPLYFACNDFVDDQIGRVINALTPEQREN
                     TWVIYTSDHGEMMGAHKLISKGAAMYDDITRIPLIIRSPQGERRQVDTPVSHIDLLPT
                     MMALADIEKPEILPGDNILAVKEPRGVLVEFNRYEIEHDSFGGFIPVRCWVTDDFKLV
                     LNLFTSDELYDRRNDPNEMHNLIDDIHFADVRSKMHDALLDYMDKIRDPFRSYQWNLR
                     PWRKDAQPRWMGAFRPRPQDGYSPVVRDYDTGLPTQGVKVEEKKQKF"
     gene            complement(4738..6453)
                     /gene="yidK"
                     /locus_tag="ACHKIR_001592"
     CDS             complement(4738..6453)
                     /gene="yidK"
                     /locus_tag="ACHKIR_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HFO9989344.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQLSDG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAMGKGSFM
                     QGIEQLTTVHAEKLNSVGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPLVGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWKNVKIAS
                     MGILFAMIGVYAGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWRHRHDPAVTFTPD
                     AKDSL"
     gene            6620..7486
                     /gene="yidL"
                     /locus_tag="ACHKIR_001593"
     CDS             6620..7486
                     /gene="yidL"
                     /locus_tag="ACHKIR_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFO9989345.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNDEQITLHGNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDIPVGQQSVIYNGEVYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMHAVGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDR"
     gene            complement(7576..9237)
                     /gene="yidE"
                     /locus_tag="ACHKIR_001594"
     CDS             complement(7576..9237)
                     /gene="yidE"
                     /locus_tag="ACHKIR_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HFO9989346.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGVGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMAMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESTRTNGGALIRTINIRVENPNLHN
                     LAIKDVPILNGDKVICSRLKREETLKVPSPETVIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASSD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFIHTLVDGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(9433..9861)
                     /gene="ibpB"
                     /locus_tag="ACHKIR_001595"
     CDS             complement(9433..9861)
                     /gene="ibpB"
                     /locus_tag="ACHKIR_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpB"
                     /protein_id="HFO9989347.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(9973..10386)
                     /gene="ibpA"
                     /locus_tag="ACHKIR_001596"
     CDS             complement(9973..10386)
                     /gene="ibpA"
                     /locus_tag="ACHKIR_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpA"
                     /protein_id="HFO9989348.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(10380..10452)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            10691..11023
                     /gene="yidQ"
                     /locus_tag="ACHKIR_001597"
     CDS             10691..11023
                     /gene="yidQ"
                     /locus_tag="ACHKIR_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFO9989349.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(11025..12239)
                     /gene="yidR"
                     /locus_tag="ACHKIR_001598"
     CDS             complement(11025..12239)
                     /gene="yidR"
                     /locus_tag="ACHKIR_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HFO9989350.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYRASQGAYVGVVTVHPKSEKYVFIHGPENPDETWYYDFHHRRGVIAE
                     GGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERVSFTYNDHVMHQLDSTLDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSNEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEAGWKAAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIACAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            12340..13404
                     /gene="cbrA"
                     /locus_tag="ACHKIR_001599"
     CDS             12340..13404
                     /gene="cbrA"
                     /locus_tag="ACHKIR_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance lipid reductase CbrA"
                     /protein_id="HFO9989351.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQHGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTMLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSAEILRSVLLKQPEKSNAAYWRATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKD"
     gene            complement(13401..14738)
                     /gene="dgoT"
                     /locus_tag="ACHKIR_001600"
     CDS             complement(13401..14738)
                     /gene="dgoT"
                     /locus_tag="ACHKIR_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9989352.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASTLWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(14813..15961)
                     /gene="dgoD"
                     /locus_tag="ACHKIR_001601"
     CDS             complement(14813..15961)
                     /gene="dgoD"
                     /locus_tag="ACHKIR_001601"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HFO9989353.1"
                     /translation="MKITKITTYRLPPRWMFLKIETDEGVVGWGEPVIEGRARTVEAA
                     VHELGDYLIGQDPSRINDLWQVMYRAGFYRGGPILMSAIAGIDQALWDIKGKVLNAPV
                     WQLMGGLVRDKIKAYSWVGGDRPADVIDGIKTLREIGFDTFKLNGCEELGLIDNSRAV
                     DAAVNTVAQIREAFGNQIEFGLDFHGRVSAPMAKVLIKELEPYRPLFIEEPVLAEQAE
                     YYPKLAAQTHIPLAAGERMFSRFDFKRVLEAGGISILQPDLSHAGGITECYKIAGMAE
                     AYDVTLAPHCPLGPIALAACLHIDFVSYNAVLQEQSMGIHYNKGAELLDFVKNKEDFS
                     MVGGFFKPLTKPGLGVEIDEAKVIEFSKNAPDWRNPLWRHEDNSVAEW"
     gene            complement(15958..16575)
                     /gene="dgoA"
                     /locus_tag="ACHKIR_001602"
     CDS             complement(15958..16575)
                     /gene="dgoA"
                     /locus_tag="ACHKIR_001602"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HFO9989354.1"
                     /translation="MQWQTKLPLIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEKSIPAIVDAYGDKALIGAGTVLKPEQVDALARMSCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALDAGAQALKIFPSSAFGPQYIKALKAVLPPDIAVFAVGGVTPE
                     NLAQWIDAGCAGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(16559..17437)
                     /gene="dgoK"
                     /locus_tag="ACHKIR_001603"
     CDS             complement(16559..17437)
                     /gene="dgoK"
                     /locus_tag="ACHKIR_001603"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HFO9989355.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLDSRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGDQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGFNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVTAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(17434..18123)
                     /gene="dgoR"
                     /locus_tag="ACHKIR_001604"
     CDS             complement(17434..18123)
                     /gene="dgoR"
                     /locus_tag="ACHKIR_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HFO9989356.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            18400..19056
                     /locus_tag="ACHKIR_001605"
     CDS             18400..19056
                     /locus_tag="ACHKIR_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010377884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9989357.1"
                     /translation="MKLNFKGFFKAAGLCSLAFALTGCITWGLVSNTASRPHAEQWRS
                     DTIKGLSLAEDSNGTKGYVFVGESLDYLLTTGGDEVVKMLNDPAIHGERITVSDNAKF
                     ILSSSNKNFSGAITLYYDWNNEEDKALATQYGFICDTRRCTWMLDGLKGSIHQKNKKA
                     DYSNVMVFHQPFTVGFYEYKATDGVPRGLVNALLPVTLTLDIVTSPLQFLILCTTRNC
                     "
     gene            complement(19100..19912)
                     /gene="yidA"
                     /locus_tag="ACHKIR_001606"
     CDS             complement(19100..19912)
                     /gene="yidA"
                     /locus_tag="ACHKIR_001606"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFO9989358.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTTLSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(20027..20425)
                     /gene="yidB"
                     /locus_tag="ACHKIR_001607"
     CDS             complement(20027..20425)
                     /gene="yidB"
                     /locus_tag="ACHKIR_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HFO9989359.1"
                     /translation="MGLFDDVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQGNQPVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            20543..20644
                     /locus_tag="ACHKIR_001608"
                     /pseudo
     CDS             20543..20644
                     /locus_tag="ACHKIR_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(20665..23079)
                     /gene="gyrB"
                     /locus_tag="ACHKIR_001609"
     CDS             complement(20665..23079)
                     /gene="gyrB"
                     /locus_tag="ACHKIR_001609"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFO9989360.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(23108..24181)
                     /gene="recF"
                     /locus_tag="ACHKIR_001610"
     CDS             complement(23108..24181)
                     /gene="recF"
                     /locus_tag="ACHKIR_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFO9989361.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(24181..25281)
                     /gene="dnaN"
                     /locus_tag="ACHKIR_001611"
     CDS             complement(24181..25281)
                     /gene="dnaN"
                     /locus_tag="ACHKIR_001611"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFO9989362.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(25286..26689)
                     /gene="dnaA"
                     /locus_tag="ACHKIR_001612"
     CDS             complement(25286..26689)
                     /gene="dnaA"
                     /locus_tag="ACHKIR_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFO9989363.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            26986..27066
                     /locus_tag="ACHKIR_001613"
     CDS             26986..27066
                     /locus_tag="ACHKIR_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9989364.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            27296..27436
                     /gene="rpmH"
                     /locus_tag="ACHKIR_001614"
     CDS             27296..27436
                     /gene="rpmH"
                     /locus_tag="ACHKIR_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFO9989365.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            27453..27812
                     /gene="rnpA"
                     /locus_tag="ACHKIR_001615"
     CDS             27453..27812
                     /gene="rnpA"
                     /locus_tag="ACHKIR_001615"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFO9989366.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            27776..28033
                     /gene="yidD"
                     /locus_tag="ACHKIR_001616"
     CDS             27776..28033
                     /gene="yidD"
                     /locus_tag="ACHKIR_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFO9989367.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            28036..29682
                     /gene="yidC"
                     /locus_tag="ACHKIR_001617"
     CDS             28036..29682
                     /gene="yidC"
                     /locus_tag="ACHKIR_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFO9989368.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISSFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            29788..31152
                     /gene="mnmE"
                     /locus_tag="ACHKIR_001618"
     CDS             29788..31152
                     /gene="mnmE"
                     /locus_tag="ACHKIR_001618"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFO9989369.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
     gene            31395..31469
                     /gene="tnaC"
                     /locus_tag="ACHKIR_001619"
     CDS             31395..31469
                     /gene="tnaC"
                     /locus_tag="ACHKIR_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006569 - tryptophan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HFO9989370.1"
                     /translation="MNILHICVTSKWFNIDNKIVDHRP"
     gene            31690..33105
                     /gene="tnaA"
                     /locus_tag="ACHKIR_001620"
     CDS             31690..33105
                     /gene="tnaA"
                     /locus_tag="ACHKIR_001620"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009034 - tryptophanase activity [Evidence
                     IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HFO9989371.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            33197..34444
                     /gene="tnaB"
                     /locus_tag="ACHKIR_001621"
     CDS             33197..34444
                     /gene="tnaB"
                     /locus_tag="ACHKIR_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HFO9989372.1"
                     /translation="MTDQSEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHAGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            34576..35751
                     /gene="mdtL"
                     /locus_tag="ACHKIR_001622"
     CDS             34576..35751
                     /gene="mdtL"
                     /locus_tag="ACHKIR_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HFO9989373.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSALFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WTMATMGIAVLMLSLFILKETRPAAPTTSDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            35726..36685
                     /gene="yidZ"
                     /locus_tag="ACHKIR_001623"
     CDS             35726..36685
                     /gene="yidZ"
                     /locus_tag="ACHKIR_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HFO9989374.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHEAWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            36842..37591
                     /gene="yieE"
                     /locus_tag="ACHKIR_001624"
     CDS             36842..37591
                     /gene="yieE"
                     /locus_tag="ACHKIR_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HFO9989375.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPTIEKLSVWELDGKHGWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKAFSPN"
     gene            37613..38179
                     /gene="yieF"
                     /locus_tag="ACHKIR_001625"
                     /gene_synonym="chrR"
     CDS             37613..38179
                     /gene="yieF"
                     /locus_tag="ACHKIR_001625"
                     /gene_synonym="chrR"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HFO9989376.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEINALPSIA
                     DIPLYDADVQQEDGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(38233..39570)
                     /gene="adeP"
                     /locus_tag="ACHKIR_001626"
     CDS             complement(38233..39570)
                     /gene="adeP"
                     /locus_tag="ACHKIR_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HFO9989377.1"
                     /translation="MSQQHTTQASGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQVGMGAIFWGAIGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMSVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            39737..40402
                     /gene="yieH"
                     /locus_tag="ACHKIR_001627"
     CDS             39737..40402
                     /gene="yieH"
                     /locus_tag="ACHKIR_001627"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HFO9989378.1"
                     /translation="MSQIEAVFFDCDGTLVDSEVICSRAYVTMFREFGIHVDLEEIFT
                     RFKGVKLYEIIDIISKEQGVTMVQADAEHVYRAEVARLFDTELEDIAGANELLASINV
                     PMCVVSNGPVSKMQHSLGKLNMLHYFPEKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSSAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            40469..40942
                     /gene="cbrB"
                     /locus_tag="ACHKIR_001628"
     CDS             40469..40942
                     /gene="cbrB"
                     /locus_tag="ACHKIR_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CbrB"
                     /protein_id="HFO9989379.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLILLVIIQ
                     VLPLFLLMSITTGAIPAMLTGVMVACLPEKIGSQKRYRCLVGGIGGVVITEIYCAVIV
                     HIKDMASSALFENILSGENLVVRIIPALLAGVVMSRIITHLPGLDISCPETDSLS"
     gene            complement(41024..41746)
                     /gene="yieK"
                     /locus_tag="ACHKIR_001629"
     CDS             complement(41024..41746)
                     /gene="yieK"
                     /locus_tag="ACHKIR_001629"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFO9989380.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKMRRVNLAITAGSTPKGMY
                     EYLITLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLHNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGVDGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASV
                     LQLHPSLIVIADKAAAAELALG"
     gene            complement(41761..42930)
                     /gene="yieL"
                     /locus_tag="ACHKIR_001630"
     CDS             complement(41761..42930)
                     /gene="yieL"
                     /locus_tag="ACHKIR_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418175.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HFO9989381.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     TDNSVTFRYFAPGAKNVSVVVGVPVPDNIHPMTKDEAGVWSWRTPALKGNLYEYFFNV
                     DGVRSIDTGTAMTKPQRQVNSSMILVPGSYLDTRSVVHGDLIAITYHSNALQSERQMY
                     VWTPPGYTGIGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPD
                     TETDAKGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFNVRKDADGRALA
                     GLSQGGYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNEPAAINQQLRNFTV
                     VVGDKDVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKLFK"
     gene            complement(42957..44573)
                     /gene="bglH"
                     /locus_tag="ACHKIR_001631"
     CDS             complement(42957..44573)
                     /gene="bglH"
                     /locus_tag="ACHKIR_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HFO9989382.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSRAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAIVMMDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTNTIDLRYKDIPLWDKATLMVSGRYVSANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIASNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKYNESGYKTTLFHTFKVNTSMLTSRPEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            complement(44659..46053)
                     /gene="bglB"
                     /locus_tag="ACHKIR_001632"
     CDS             complement(44659..46053)
                     /gene="bglB"
                     /locus_tag="ACHKIR_001632"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HFO9989383.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWQEDGKGITTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMPYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVVLWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKACHSLIP
                     EAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQIDPVGLRILLNTLWDRYQKPLFIVENGLGAKDSVEVDGSIQDDYRIAYLNDHLVQV
                     NEAIADGVDIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKKSFGW
                     YAEVIKTRGLSLKK"
     gene            complement(46072..47946)
                     /gene="bglF"
                     /locus_tag="ACHKIR_001633"
     CDS             complement(46072..47946)
                     /gene="bglF"
                     /locus_tag="ACHKIR_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HFO9989384.1"
                     /translation="MTELARTIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDDKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFWFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKVDALGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPITFLLVGPLSTWISELIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLIPLIINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPGIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTVMFH
                     FITAKSQSAQVAQQEKTPEVITPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIAI
                     MPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNVG
                     DKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQINAGEPLLSIIR
                     "
     gene            complement(48080..48916)
                     /gene="bglG"
                     /locus_tag="ACHKIR_001634"
     CDS             complement(48080..48916)
                     /gene="bglG"
                     /locus_tag="ACHKIR_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HFO9989385.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRPGERINSS
                     GIEKEYALSSHELNGRLSELLSHMPLEVMATCDRIISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIVKRLGVQLPKDEVGFIAMH
                     LVSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRIIE
                     HASINDSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRK
                     EH"
BASE COUNT        12039 a        12838 c        12145 g        12069 t
ORIGIN      
        1 aaaaaccccg ccggagcgag gtttcgtcag tcgcctgcgg ctggtaaccg caaagcacac
       61 tgtattatgt caacactgaa agtatacgtg ttccgcgcag aacgcgcaat ttcggcacga
      121 attttgacgt atttagtgca tagttgagta tcgatcacag tttgcgtttt gtccaaaatt
      181 actgtttatt tatacagtaa acttctataa tatcactgta cgcaatgtgt tatgcggggg
      241 ccgcatcgtt acccggcgca ctaagtcctg gcagaaacgg gtggtgccgt cagcgcctta
      301 accccgcgtg agcacactgt gttatgtcaa caagcacaac gtttctcctt gagataccgc
      361 gtgcacaaca gctggcaaca ggcagcggaa aggtacgtca gctggcagtg ctcctgaacc
      421 acaggagacg cgtatgagcc tggtggatat cgccattctt atcctcaaac tcattgttgc
      481 agcactgcaa ctgcttgatg ctgttctgaa atacctgaag taattcagat tcaagtcgca
      541 ctaaagggga gcgggtaacc gctccccttt tatatttagc gtgcgggttg gtgtcggatg
      601 cgatgctgac gcatcttatc cgccctacca tctctcccgg caacatttat tgccgctttt
      661 gtttacatat tctgccgcta aacaattccc cattcctggc gtatatctgg ctaacattca
      721 tcaatgtgat agattcctct cccgcattta tgggaatgcg tagtgactta ttctaattat
      781 ttttataaaa gcatccgtga taatgaaaag gcatagaaac gtcaatttgt tattgatgtt
      841 ggtattactc gtggccgtcg gtcagatggc gcaaaccatt tatattccag ctattgccga
      901 tatggcgcgc gatctcaacg tccgtgaagg ggcggtgcag agcgtaatgg gcgcttatct
      961 gctgacttac ggtgtctcac agctgtttta tggcccgatt tccgaccgcg tgggtcgccg
     1021 accggtgatc ctcgtcggaa tgtccatttt tatgctggca acgctggtcg cggtcacgac
     1081 ctccagtttg acggtattga ttgccgccag cgcgatgcag gggatgggca ccggcgttgg
     1141 cggcgtaatg gcgcgtactt tgccgcgcga tttatatgaa cggacacagt tgcgccatgc
     1201 taacagcctg ttaaacatgg ggattcttgt cagtccgttg ctcgcaccgc taatcggcgg
     1261 tctgctggat acgatgtgga actggcgtgc ctgttatctc tttttgttgg ttctttgtgc
     1321 cggtgtgact ttcagtatgg cccgctggat gccggaaacg cgtccggtcg acgcaccgcg
     1381 cacgcgcctg cttaccagtt ataaaacgct tttcggtaac agcggtttta actgttattt
     1441 gctgatgctg attggcggtc tggccggaat tgccgccttt gaagcctgct ccggcgtgct
     1501 gatgggcgcg gtgttagggc tgagcagtat gacggtcagt attttgttta ttctgccgat
     1561 tccggcggca ttttttggcg catggtttgc cggacgtccc aataaacgct tctcaacgtt
     1621 gatgtggcag tcggttatct gttgcctgct ggctggcttg ctgatgtgga tccccgactg
     1681 gtttggcgtg atgaatgtct ggacgctgct cattcccgcc gcgctgttct ttttcggtgc
     1741 cgggatgctg tttccactgg ctaccagcgg cgcgatggag ccgttcccct tcctggcggg
     1801 caccgctggc gcgctggtcg gcggtctgca aaacattggt tccggcgtgc tggcgtcgct
     1861 ctctgcgatg ttgccgcaaa ccggtcaggg cagcctggga ttgttgatga ccttaatggg
     1921 attgttgatc gtgctgtgct ggctaccgct ggcgacgcgg atgtcgcatc aggggcagcc
     1981 tgtttaagct cacgtcaccg cagcatcgtc atcagctcca tgggagaacg atgctgcttt
     2041 atcagatcac gcatcacccg catatgcggc gcggagtaag aataaaacgc ctgatagccc
     2101 gcacaaagca cgttgtctgg tttatcttcc cgagcgtgcg ggcacagccg atgccagtcg
     2161 cacgcctggc agtcacggcg cggttgcgac ttttcgctgc cacaccattt ttgtaaggtg
     2221 gtgtcaaaaa gttcaatcga aatacgactg atatctgccc gcacccaaca ctcgaacagg
     2281 gcaattaaaa atttccccca cagctctgct gtcaccgaag cggccgtcag atcgccccgg
     2341 tcatcaaatt caagatgggg aataagctgt actgagcgga tgcccagctg tttgaaattg
     2401 tgataaaaat cggcaggttg cagagcatcc ttatactcta caaccagttt atgcctgtcc
     2461 tcttcagcat caatgacctg acttcctgtc atgcgactct ctcaataaat acgataagtt
     2521 gatgtatatg cgttacagca aacagtattg cgagagataa caccgcgagg gagatcaaaa
     2581 atttgtcacg gactacgtga aattgggaaa aatcgctatt cgtgacatcc attaaattgc
     2641 gattacgggt gtagtgccag aggatcagcg ccacgatggc gaggatgcca atcgaaatcc
     2701 agaataacat gcccgcctgg tgccagttgt gtttgatagc caacgccatc aacgcgccgt
     2761 agcccagcat ggtacgaaac cacgccagtg atgtgcgttc cggctgcagc cctgggtcgg
     2821 caatgcgtct ggctttgcgg ctatccggca tacaacacca gtcccatgac gatcactgcc
     2881 acgaccatca atattaaact gatgattaat aagctgttgg tatagggcag gtcctctttc
     2941 aggcgcatcg ctttttcatt gcgtaaccag cgcaagtagc catacatcgc taacccgccg
     3001 gaaaacagac acaacaacaa cgccagcagt tcgcggatga cgggcgtggc gaaatctggc
     3061 gcaagttgat caagcccaac ccccgccgcc agaaatccta gtgcagtacg gatccacgcc
     3121 agaaaagtac gctcattagc cagcgagaag cggtaatcgg gcgcttctcc gaggcgggaa
     3181 attttcattg ttgctccttg taaactgttc ctcgccgaaa aattaggcca ggaacagcaa
     3241 taatgcatca gaacttctgt tttttctctt ccaccttcac cccttgtgtc ggtaaaccgg
     3301 tgtcatagtc gcgtaccact ggagaataac catcctgtgg gcgtggacga aacgccccca
     3361 tccagcgcgg ttgtgcatct ttgcgccacg gacgcaggtt ccattgataa ctgcggaacg
     3421 gatcgcgaat tttgtccata taatccaata aggcgtcatg cattttgctg cgaacgtcgg
     3481 caaaatggat atcatcgatc aggttatgca tttcatttgg gtcattacgg cgatcgtaaa
     3541 gttcatcact ggtgaagagg ttgagcacca gtttaaagtc atccgttacc cagcaacgca
     3601 ccggaataaa accgccaaag ctgtcatgct caatctcgta gcgattaaat tccaccaaca
     3661 cgccacgtgg ttcttttacg gcaaggatat tatcccccgg cagaatctct ggtttttcaa
     3721 tatctgccag cgccatcatt gtcggcagta agtcaatatg actgactggc gtatcgacct
     3781 gtcgccgctc cccttgcggc gaacggatga tcagcggaat gcgggtgatg tcgtcataca
     3841 tcgccgcccc tttactgatc agcttatgtg cgcccatcat ttcaccatga tcggaggtat
     3901 aaatgaccca cgtattttca cgttgctctg gcgttaaggc attgatgaca cgtccgattt
     3961 ggtcatcaac aaagtcattg caggcaaaat agagcggatg gtgataaagc ccgtcatcac
     4021 cgactggcga tggcatcgcc tgcgcccata agcggtgatg ttccggtttg ttcgccaggt
     4081 catcctgtga tttctcgcca agttcgtagt aaaaatcagc gtatttctct aaatactcca
     4141 ccggacaggt gaacgggtga tgcggctcat catacgaaac caccatcagg aaaggttcgt
     4201 cggcgcgcgc gggctgttgc agaaaatcta ccgcccgatt gctgatgcgg tgcgcccagg
     4261 tgaaggtttc gtcgatatgg ttcgcctgta aatcctcaac gctgtttagg ccattgcgcc
     4321 acaggctgat ctctttttcc gtcagttcgc taaggtagtt cgccccatcg aaccagtaat
     4381 cagcgtccca ctccggcgga cactcgccag tgccgaaata gtcatgaccg tcgagatgcc
     4441 atttgccaat gtaacaggtg tgatagcccg catccttaaa gtagcgcccc atggtggaaa
     4501 tgtttttacc tggcgcgacg ttgttggtcc acgggccgga ctggttagcg tagataccgg
     4561 taaacagccc ggcgcgcgct ggtgtacaaa ccggtgaaca ggtataggcg gaattaaagc
     4621 gaatactttc cgccgccaga ctatcaatat tttgcgtatt cagcggctta ccgctatagc
     4681 aaccgaccat attggtggcc tgggtatcgg tcatgatgaa cagaaaattg gggcgtttca
     4741 taggctatcc ttcgcgtcgg gagtaaaggt tacggctggg tcgtgacgat gccgccagct
     4801 atcaaaaatc aggtagacaa tcactacggc ggcaatgaaa taactgatca tcgctaacca
     4861 gcgcgtaccg tagccgccga attcagccag cccggcatag acgccaatca tcgcgaacag
     4921 gatgcccatt gacgcgatct tgacgttttt ccacggtttc atgtccaccg caaacgcatc
     4981 tttgaaggtg aacggcgtgg cgcgcggttt gataaaaccg atcaccagca tcacgaccac
     5041 gttgatgcag aacgtacagg ccagcacata gaggaaatgg aagtcgaact tcaccagata
     5101 gttgatggtg atgtagctga ttatgccaat ccccatcgcc acttttgccg ccagcgccgg
     5161 gatgcgcggg aagaaaaagc ccataatgat gatggtaacc agcggcacgt tgtaaatgcc
     5221 gttgagctgt ttcatccagc tatacagccc ctgcggcgcg ttggcgatcc acggcgctac
     5281 cagcaccgaa acgatggcga taaagaaacc aaatttgcgc ccgacggtga ccagctgctg
     5341 cggctcggca ttctggttaa tgatgcgccg gtaaataccc atactgaata acgtactggc
     5401 gctattcaga aagccgttga aggtactgat caccgcacca aataacaccg cgccgaagaa
     5461 acccaccagt ggcactggca gaacgttatt gaccagcgtc gggtaggcca tgtcggcttt
     5521 cggtagatcc tgatacagat gaaacgcaat caaccctggc agcaccagta ccagcgggtc
     5581 gagcattttc agcaccgccg ttaacagcgc ccccttttgc ccttccgcca ggctttttga
     5641 cgccagcgtg cgttgcacga tgccctgatt tgtacaccag taaaaggtgt tcaccagaat
     5701 caaaccggta aatgccgcgc caatcggcaa gggatcggtc gggccgccga ctgagtttaa
     5761 tttctcggcg tgaacggtgg tgagttgctc aatgccctgc ataaagctgc ccttgcccat
     5821 cgcgatcagg ccaaataccg gcaccatcaa cccgccaata accagcccaa taccgttaat
     5881 ggagtccgcc actgccattg cgcgcagtcc gccgatcacc gcatacaaaa ttcccgccag
     5941 accaagcaaa ataaccagta accagatagc cgcaccatcg gaaagttgta gcgactcacc
     6001 cacatgaaac aggctgttga gcgccaacgc gccggaatag agaacaatcg gcagaaagca
     6061 gacgccggtg gcaatgagga agcagaagtc gatgataata cgcgtcgttt tatcataacg
     6121 ttcctccaga aaatcgggga tggtggcaat gccgcgcttc agatagcgcg gtaaaaagat
     6181 tagcgcgagg aagatcagcg tcactgcaga ggtgacttcc cagcccatca ccgacatgcc
     6241 acttttgtag gcctgcccag aaaggccgac cagttgttcc gtggaaaggt tggttagcat
     6301 taacgaagcg gcaataaccg gcgcttttag tgaacgtccg gcaagaaaat agccttgttg
     6361 cgatccggta tctgttttac ggaccttcca ccaggtgatg accgctacca gcagcgtaaa
     6421 accgacaaaa ctcaagattt gtaacgaatt catctcatag cccttattta tgtttatgtc
     6481 ccggacgcat gaatataggg agaggtaaaa tgcgtcgggt caggcagtca gattcttaaa
     6541 atacaaacgt cgtatccctg aacggattgt tttctgttaa gttcaggttg tgagcataat
     6601 tcgccggagg gatattaaaa tgaatggaaa attgcaaagt tcggatgtaa aaaacgaaac
     6661 tccgtacaat attcccttat tgattaacga aaatgtgatc tccagcggaa tttctctgat
     6721 atcgctctgg catacctacg ccgacgagca ttatcgggtg atctggccgc gggacaagaa
     6781 gaaaccgctt atcgccaact catgggttgc ggtgtatacc gtacaaggat gtgggaaaat
     6841 tcttttaaag aatgacgaac aaataactct gcatggcaac tgtattatat ttttaaagcc
     6901 aatggatatt cactcttatc actgtgaagg tttggtctgg gaacagtatt ggatggaatt
     6961 tacccccacc agtatgatgg atattcccgt cggtcagcaa agcgttattt ataatggcga
     7021 agtttataat caggaactca ccgaagttgc tgagttaatt acttcaccag aagcaataaa
     7081 aaataatctg gcagtcgctt ttctgacgaa aattatttat cagtggattt gtcttatgca
     7141 cgcagtcggt aaaaaagatc cacaacggcg gcaaattgaa aaattaattg ccactttaca
     7201 tgccagtctg caacaacgct ggagcgtagc tgatatggct gccacgatcc cctgtagcga
     7261 ggcctggttg cgtcgtctgt ttttacgcta taccggcaag acgccgaaag aatattacct
     7321 cgatgcgcgt ctggatctgg cgctatcgct attaaagcaa caagggaact cggttggcga
     7381 agtcgctgat acgctcaact tcttcgactc ctttcatttc agcaaagcct ttaagcataa
     7441 atttggttat gcgccgtcag ctgtgctgaa gaatacggac aggtaataac cagaatggcg
     7501 agtgtgtaaa tgactcgatg atgttcagaa ataccaggtg gagtaccatt tacggcgact
     7561 ccacccggaa aagagttaac cgatactcca gaagagcacc gccagtaatt ggggggtgat
     7621 aatgcgcagg aacatcacca acggatagac agtggcgtaa gagagcgccg ccgcaccgct
     7681 cgttggatga agattattag caaatgccag cgccggagga tcggtcatgg agccagccag
     7741 catcccgcac atggtcaggt aattcatttt ggctaacatc cgcgccagaa tgccaacagt
     7801 aatcagcgga acggcggtga tcagggcacc ataaccaatc cagcttagcc cttcgccatc
     7861 caccagtgta tggataaaat cgccgccgga tttcaggcca accaccgaga gaaagagcac
     7921 aatgcctaac tcgcgtagcg ccaggttagc gcttggtggc ataaaccagt acagcttgcc
     7981 gatactgccg atacgcccga ggatcaacgc cataatcagc ggaccgcccg ccagcccgag
     8041 tttcaacgcc gccgggaacc caggcacaaa gacgggaata gaacctaaca atacgccaag
     8101 cccgatacca ataaacactg gcagcatctg aacctgttgc agtttttgct gcgcattccc
     8161 cagcacattg gcaacggcat caattgcgga cggacgcccc accaggttga ggatatcgcc
     8221 gaactgcagg ctgatatcgc tacttgcaac taattcaaca ccagcacggt tcaggcgcga
     8281 gataacaacg tcatagcgtt ctttaaagtg caggtcgcga atacgtttac cgagcacgtt
     8341 ttcattggtg accaccacac gctcgacgcg caagtcagtg cctttcgtag acagcgaggt
     8401 atcgacctcc tgaccaatca ccagttgcgc attatgtaaa tccgcaggct gccccaccag
     8461 atgcagcaaa tcgcccagtt gaataacggt ttctggcgaa ggcactttta gggtttcttc
     8521 tcgtttcaat cgtgagcaaa taactttatc gccattgaga atcggcacat ctttaatggc
     8581 taaattatgc aaattaggat tctcaactcg gatgttaatc gtcctgatta gcgcaccgcc
     8641 gttggttcgc gtggactcgt gctgctgggc ctcagtctca acattgacgc ggaaaataac
     8701 ccgcagcatc cacatagtga acaaaatgcc gcaaatccca aatggatacg ccatcgcata
     8761 gctcatcccc atttgatcga ccatcgccat cggtgtcccc aggtcgcgca agatctgctg
     8821 ccctgccccc agtgctggcg tattggttac cgcaccggag aaaatcccca gtactaccgg
     8881 cagtggaata tcaaacagtt tatgcaggat ggcggtaacc agccccccga tgatgacgat
     8941 cagaacagca aacaggttga ggcgtaatcc ggagacgcgc aatgaggcaa agaagcccgg
     9001 ccctacctga atcccgatgg tataaacgaa caggatcagg ccaaattcct gaataacatg
     9061 cagcatatcg ctacttaatg tcatccccgc ctgagaaaca aaatggccga cgatgatccc
     9121 accgaacagc acgccgccaa tgcccaatcc aacaccgcga aacttgacgt tgccgataaa
     9181 caacccgacg accgccacca aagccagaat actgaccgtt aatgctatat cactcatcgt
     9241 ccatttctct tgagataaca ttttaattgc tacggattat ggctgaatcg gtgaagcaga
     9301 tttgccttat agcgcacatt atcacgggaa agaacgtgcc gaaatatctt aaacaggcga
     9361 aaattaacgc attgttgaag aagaaagaca aaaaaagccc caccagaatg gcggggcaaa
     9421 gagaatagct agttagctat ttaacgcggg acgttcgctg atagcgatac gctgcgctgc
     9481 gatgggttca ggctcattac gaattaaatc aatatgcagt aaaccgttta cgaaggttgc
     9541 accagagact tccatatttt cagccagcgt aaagctcagg ctaaatggct gattcataag
     9601 cccttgatgc agccattttt tctcttcttt tggctgctcc ggcgtgcctt ttacgctcag
     9661 gcgcgtacct tccagttgaa tctctaaatc ttcctgacga aaacctgcca gcgcaagggt
     9721 aatgcggtag tggttatcgt cgcttttctc aatgttgtac ggcgggaagc tctggctttc
     9781 accggcgttt tgcagtgcgt tggccagttt gtcaaaaccg atccattgac gcatcagtgg
     9841 ggataaatcg aagttacgca tagtcatttc tccttctaag aagcgagtaa gtacctgcaa
     9901 atccgaagat tcgcatatgc tccctgacgg cgagcatgga gatgtcaggc cgcgcaaggc
     9961 ggccttaggg aattagttga tttcgatacg gcgcggtttt ttcgcttccg gaatcacgcg
    10021 ttcgagatcg atatacagca aaccatttac caggttagca ccacgaacat gaatgttctc
    10081 agctaactgg aatttgcgtt caaagttgcg ttcagcgatg ccctggtaca gataggtgcg
    10141 ctctttttgt tcgtcggcgt gagcaccttt caccaccagc agattatcct gggcggtaat
    10201 ttccagttcg ctctcagcaa aaccagccac agcgatagca atgcggtaat ggttttcgtc
    10261 taccagttca acgttatacg gagggtagcc gccattactc tggctctggt tgttttctaa
    10321 gtggttaaac aaacggtcaa atccaatagc agaacggtaa agcggggata aatcaaagtt
    10381 acgcataatc aatagctcct gaaatcagcg agaatctaag accttccaca atggacaggt
    10441 caggtagcca gaacacccat caggcgcgtc ctcatcggct acgatgtaaa aatgggtctg
    10501 gaaatgaact tttcaagcct tatcagcgga ctttttttgc agtttatggt ctattgcata
    10561 gactgagggg gcagcacgaa acgttaaagt gcgatagccg ccacagtgcg acgtaatgat
    10621 gatgttattt tcagcaaggg atcgctataa ctcatcaggc caacacccac gatcaacaga
    10681 tgaatttatt atgataagaa atgtgttgtt agcgttcatg atatgcagcg gaatgacatt
    10741 acttggagga tgctccagcg tgatgtccca taccgggggg aaagaaggaa cgtatcccgg
    10801 cacccgcgcc agcgctacca tgattggcga cgatgaaacc aactggggca ctaagtcgct
    10861 ggcgattctc gatatgccgt ttaccgctgt gatggacacg cttttactgc cgtgggacgt
    10921 gttccgcaaa gacagctcgg tgcgctcacg cgttgaaaaa agcgaagcta acgcgcaagc
    10981 caccaatgcg gttattccac ccgccagaat gcctgataac tgatttagcg atcagtttcg
    11041 gtgatccaca gctggccgcc ttccatccac gccagccact gaccatccgg cgagaagacc
    11101 acggcgtccg cagaaggtgg attggcgtgg ttttcggtta aatactcaac ctcgccgctt
    11161 tgcgcatggg cgcaagcaat tcgattatcc agcacaaagc ccaaccattc tcctgatgga
    11221 tgccagttaa atgcagactg aatatccgtt ttgttatggg ttaactggcg cggctcgccg
    11281 ccctgtggcg agataagcca cagttgcaca atgccgttat catcacgcat taaaaacgcg
    11341 atttgcgtac cctgcggatt acagcgcacc cagtggcgcg ggacgttgac taaccccgga
    11401 taagcccgat ggtgggtaaa ggttaaacgt cgctgcacga cgccacgcgg tggcgcgggc
    11461 agggttgttt ccgttccact taacggcgca tcaccagctg ctttccagcc agcttcatct
    11521 tgcggtaact caacgataaa tagctccggc actttctcgc cctttggcga aagtgtgtcg
    11581 ccaataaacg ccagcgcgtg atttcctacc catccttctt cataagcacg attgatttca
    11641 ttactgccag gctgcggcgt gggcgttgtt ttactcacca gcacgcacca gtggctaccg
    11701 ctgtattcac gcggatgctg cttttgtacg ttgaccgggc caaacggcgc agcaacacct
    11761 acgtttcgca aatccagcgt cgaatcgagt tgatgcatta catggtcgtt ataggtaaag
    11821 ctcacccttt ctccgttcgg gctaaagaca tgcacatggc tgccgccgcg cagcgctcct
    11881 ggggtgtacg gcgcggtaat atccattgca tcgagattgc tcaccttgcc gccttcagca
    11941 atcactccgc gccgatgatg gaagtcgtaa taccatgttt catcaggatt ttccgggccg
    12001 tggatgaaaa catatttctc tgacttcgga tgaacggtca ccacgccgac gtacgcgccc
    12061 tgtgacgcgc gatagataac ctcgacctcg ccggtatgga tattcacacg ctcaatggtc
    12121 tcgccggtaa acgacgcccc tgaaggacgc acgtcaaata ccagccactg gctatcgggc
    12181 gtccaggtat tggtattggt gagcaggtga ttacggggag caaaggttat ctgtttcatg
    12241 gcgcggtcct gatacaaaac gggtcccgcc atcatacttc acaaggactt cactttcaga
    12301 caatagtctt ttgccatcgt gatgaatgtg agtgaaaaga tggaacattt cgacgtggcg
    12361 attattggcc tcggcccggc agggtcggcg ttggcgcgaa agttagcagg caaaatgcag
    12421 gtgatcgcgc tggataaaaa gcaccagcat ggtactgaag gtttcagcaa accttgtggc
    12481 ggtctgctgg caccggacgc gcagcgatct tttattcgcg atggactgac gcttcctgtc
    12541 gatgtcatcg ccaacccgca gattttcagc gtcaaaaccg tcgacgtcgc cgcatcgctc
    12601 acgcgtaact accagcgaag ctatatcaat attaatcgcc atgctttcga cttgtggatg
    12661 aaatcgctga tccccgccag cgttgaggtt taccacgaca gcctgtgtcg aaaaatctgg
    12721 cgtgaggatg ataaatggca tgtcattttt cgcgcagacg gctgggagca gcatattacc
    12781 gcccgctatc tggtcggtgc cgatggtgcc aactcgatgg tgcggcgaca tctctacccg
    12841 gatcatcaaa ttcgtaaata tgtcgctatc cagcagtggt tcgcagagaa acatccggtg
    12901 ccgttctact cctgcatctt tgataatgcg ataactgact gttattcatg gagtatcagc
    12961 aaagacggtt attttatctt tggcggtgcc tatcccatga aagacggtca gacgcgtttc
    13021 acgatgctga aagagaaaat gagcgccttt cagttccagt ttggtaaggc ggtgaaaagc
    13081 gaaaaatgca cggtgctgtt tccctcacgc tggcaggatt ttgtctgcgg taaggacaat
    13141 gcctttctga ttggtgaagc ggcgggattt atcagcgcca gctcgctgga ggggattagc
    13201 tatgcgctgg atagcgcaga gattctgcgt tcggtgttac tgaagcagcc ggagaagagc
    13261 aacgccgcct actggcgcgc cacccgcaaa ctgcgtttaa aactcttcgg caagatagta
    13321 aaaagccgat gcctgaccgc accggcttta agaaagtgga ttatgcgcag tggtgtggcg
    13381 catattccac agttgaaaga ttagccaacg cgcttcacat cgcccaccag caggatgtaa
    13441 gagagcgcgc caatcaacgc gacggcggag atataaacca gtgcaggtgc gaaaccgtaa
    13501 ccctgcgcca ggtaccccac caccagcgga acggtgatgc cgcccagacc accggcgaag
    13561 ttaaacacgc cgccggttaa accaatcagg cgcatcggtg ccagagaaga gaccagcgac
    13621 caggtaatcg aagcaaaacc gttgccgaag aatgccagcg ccatcaggca cataatcatc
    13681 atcggatcgt tggtgtagtt agcgcccata atgcaggtgg agatcagcaa gccgcagata
    13741 atcggcgttt tacgcgcaaa gcccagtgaa aagcccttac gtaccagcag atccgcgacc
    13801 cagccagaga gcaggacgcc gacaaacgcc gcgaggaatg gcaccgtggt cataaagcct
    13861 gctttcagcg ccgtgattcc tttttcctgg gttaaatagt tcgggaacca agttaagaaa
    13921 aaccacagtg tagaagccac cgcaaattgc ccaagataga cgccgatcag tttacgatgg
    13981 aacaccagtt tccagtcttt ggctgttaac ggctgacgcg cctctttttt caccggcgca
    14041 tcaccatcca ccagaccgcc gccatcacga atgtaatcca gttcagcttt gctgatgcct
    14101 ttagtcaggc gcggcggctg ataaacctta aaccaaatca gcgaccagat aatgccgata
    14161 ccaccggtga caatgaacac ccagtgccag ctcaacattt cctgaatcca gatcagcagc
    14221 ggagtcaaaa acgccagacc gacaaactga ccagacgtat aaaaaccaac ggcggaagcg
    14281 cgttcatgtt ccgggaacca gctggtcacc atccggttat tggtcgggaa agcgggcgct
    14341 tcgaaaatac cggttattgc gcgcagacca attaatgaca tcaatcccgt ggcaaagccc
    14401 tggaataaag tcgccactga ccagccaaat atcgcaataa aataagtcac gcgtgaacct
    14461 acgcgatcta aaaaccaacc gccggggatc tgacatagtg tataaagcca ggcgaaggcc
    14521 gaaaatacat agcccatttc cgctttggta ataccgaact cttcctgaat atgggcggaa
    14581 gccacggcca ggttagcgcg gtcaacataa caaatgacca ccgtaataaa gatcatcacc
    14641 agcgtcagat aacgccgacg ccccggcttt gctgcattaa cgggaatatc catagcgagc
    14701 tttctccaga ttttgggcat agcgaagccg ctcaccatgc cctgtaattt acagagggtt
    14761 atatttttgt attgctgttt tagtgcccga tgagaggctt acgtggcagg aattaccact
    14821 ctgctacgct gttatcttca tgacgccaga gcggattacg ccagtccggg gcatttttac
    14881 tgaactcaat aactttagct tcgtcgattt ccacacctaa gcccggtttc gttaatggtt
    14941 taaagaaacc gccgaccatg ctgaagtctt ctttgttttt cacaaagtcg agtaactccg
    15001 cgcctttgtt gtaatgaatg cccatacttt gttcctgaag tacggcgtta taggaaacaa
    15061 agtcgatatg caggcaagcc gccagtgcaa tcggtccgag cggacagtgc ggcgcaaggg
    15121 tcacgtcata ggcttctgcc attccggcaa ttttgtagca ttcggtaata ccgcccgcgt
    15181 gggagagatc tggttgcaga atcgaaatac cacccgcctc cagcacacgt ttaaaatcga
    15241 aacgtgagaa catgcgttca cccgccgcca gtggaatatg cgtttgtgcc gccagtttcg
    15301 ggtagtattc ggcttgttct gccagcaccg gctcttcaat aaacagcggg cgatacggct
    15361 ccagttcttt aatcagcact ttcgccatcg gcgcgctgac gcgaccgtgg aaatcaagac
    15421 caaactcaat ctgattgcca aaagcttcac gaatttgtgc cacggtgttg actgccgcat
    15481 ctaccgcgcg ggagttatca attagcccca gttcttcaca accgttcagt ttgaaggtat
    15541 cgaagccgat ttcgcgcagg gttttaatgc cgtcgataac atccgccgga cgatcgccgc
    15601 cgacccaact gtaggcttta attttgtcgc gaaccaggcc gcccatcagt tgccagaccg
    15661 gcgcattcag cactttgcct ttgatatccc ataacgcctg gtcgatcccg gcgatggcgc
    15721 tcatcaggat tggaccgcca cgataaaatc cggcgcgata catcacttgc cataagtcat
    15781 tgatgcgcga tggatcctga ccaatcaaat agtcacccag ctcgtgaact gccgcttcca
    15841 ccgtgcgggc gcggccttca atcactggct cgccccagcc gaccacgcct tcatcggttt
    15901 caattttcag gaacatccag cggggaggta aacgatacgt ggtaattttg gtgattttca
    15961 ttgcactgcc tctcgatacg ccttaacaaa tgctgctgcc tgctgcgcgg tgcgttctac
    16021 ggactgcccg gcgcgataga gatcgctgcc taagcccgcc cctgcacaac ctgcgtctat
    16081 ccactgcgcc aggttttctg gcgtcacgcc gccaacggca aagactgcga tgtccggtgg
    16141 taataccgct tttaacgctt tgatgtattg cggaccaaaa gccgatgacg gaaatatttt
    16201 cagcgcctga gcgcccgcgt cgagcgcggt aaaggcttcg gtcgccgtcg cgcagccggg
    16261 gcaaacggtc atgccgtagc ccaccgcacg gcggatcact tcactatgga tattgggcgt
    16321 aacgatgagc tggcagctca tcctggcgag tgcatcgacc tgttcaggtt tcagtaccgt
    16381 acctgcgcca atcaacgcct tatcgccata cgcatcaacg atggcgggaa tgcttttctc
    16441 ccattgaggg gaattcagcg ggatttcaac cgcgtcgaac ccggcgtcaa tcaccgcgcc
    16501 aacatgcgcc agcgcctcgt cgggcgtaat gccgcgcaaa atggcgatca gcgggagttt
    16561 agtttgccac tgcatgagcg atgctcctta taccagcctg aaatgccgtg tcgcccgcca
    16621 ccgccgtcac gtcgcaaccc atcgcctgaa aggcttgctg gtagcgcgcg gtcagcgatg
    16681 ttccggcgac aagggtgatg gcgtgttgat gggtcacata gtcgcgcata ctggcaacct
    16741 ctgcgccaat caacaaacca gagagaaatt cgctgacctg ttcgcgggga agtgttccca
    16801 gcacatgcga ggcgcgaact tcaaaaagct gcggcaatat ggcgggagca ttaaagccac
    16861 gctcaagacc agccgcgaag gcatcggcag agttttcctg cggcggcaaa cctgcgccaa
    16921 tcaatgagtg atttaacagt aaatgatgta attcaccggt catcacggtg cgaaaatcgt
    16981 tgatttgctg gctatcggcc tgcacccatt tgcaatgggt accgggcata acataaagag
    17041 aggaaggagc gagagcacgc gcgccgatca attgtgtttc ttcgccgcgc atcacattgt
    17101 ggttatcgtc atgagagacg cataatccgg gaataatcca gatattgtcg ccaaccgacg
    17161 ttaattgatc gccaatagac gaaaaacggg caggaacaga taaatacggt gcaactttcc
    17221 agccgacatt gctgccgacc attcctgcca tcaccactgg cgtattctct tcacgccagt
    17281 cggtcgtgac ttctgctaac accgcagccg gagattttcc gttcaggcgc gtgacgcctg
    17341 cttctgattg cctgctgtcc aggcagtggt cgccctgata aagccaggcg cgcagattgg
    17401 tcgatcccca gtcaattgcg atgtagcgag ctgtcatgtg atttccttta accttcgtgt
    17461 cgagctggcg atcatggtaa gcgccgcctg ctctgccgca tcgccgtcct gatgccgtat
    17521 tgcatcgaac agcgccttat gttcctggag cgtttgcggc atgttggcct catcgcccat
    17581 ccaggttcgt tcaaaaaccg cccgctgcag cgaactgatc gcaatgctaa gttgctgtaa
    17641 caccgggtta tgcaccgact gcagcaccgc ctcgtggtag cgaatatccg cttcgttaaa
    17701 cgcttcgcgg tcctgattgt tggcaatcat ctcgttcagc gccgattcaa tctgcgccag
    17761 atcgctggag gtcgcgcgct ctgctgccca acgggcaata gccggctcta ccaggtttcg
    17821 cacttcgctc attgcactga taagccgtgg gtcatagtcg ttttccagca cccattgcag
    17881 tacgtcagtg tcgaggtaat tccactggtt acgcggagcc acaaacgccc cgcgataacg
    17941 tttcatttca atcagccgct tcgccatcag cgaacgaaac acctcacgga tgatgttgcg
    18001 cgaggttgca aactcttcac agagctccgc ctcagccgga agtggagagc ctggtacgta
    18061 tttgccgtga acgatctgtt tacccagcgt aataacgatg cgatcggttt tattgagagt
    18121 catggagagt ccttgtgctc tgtatgttct tctctacttt accccgatcg atgcataacg
    18181 cggcaacttt gtagtaccag cgtgatgacg ttcgcgtttg ccgtgcgcct aatgtagtac
    18241 aaacttatat tgttgtacta caatttagat cacaaaaaga acaatacata aaaaatgaca
    18301 tgcgtcgggc agaaatctga aaagggatgt caggcgctaa acaggaggga aagaagagta
    18361 tgctttcaac ggcttagcta ctcgtttaaa ggattaatca tgaagttgaa ttttaaggga
    18421 ttttttaagg ctgccggttt gtgttctttg gcatttgcgt tgaccggatg tatcacctgg
    18481 ggattggttt ccaatactgc aagccgtcca catgccgagc aatggcggtc agacacgatt
    18541 aagggtttat cgctggctga agacagtaac ggtacgaaag gctatgtttt tgttggtgaa
    18601 tcgttggact atctgttgac caccggcggc gatgaggtgg ttaaaatgct gaacgatccg
    18661 gcaatccatg gtgaaagaat caccgtttct gataacgcga aatttatttt aagcagcagt
    18721 aataagaatt tctctggtgc tattacgctc tattacgact ggaacaacga agaagataag
    18781 gcactggcaa cacaatatgg ttttatttgt gatacccggc gttgtacatg gatgctggac
    18841 ggtttgaaag gctcaatcca ccagaaaaat aaaaaagcgg attactcaaa cgtgatggtt
    18901 ttccatcaac cctttacggt gggattttat gagtacaaag caacagatgg cgtcccgcgc
    18961 ggcctcgtta atgcgctgct gccagtcact cttacgctgg atatagtaac gtcaccactc
    19021 cagttcctga ttttatgcac gaccagaaat tgttaatcgc tgcgggcaaa cgcattcatg
    19081 ccgtttgccc attcactaat taattcagca catacttctc aatagcaaat gccacgccat
    19141 cttcaaggtt ggatttggtg acaaagttcg ccacttcttt caccgaagga atagcgttat
    19201 ccatcgccac accgacgcct gcatattcaa tcattgcgat gtcgttttcc tgatcgccaa
    19261 tcgccatgat ttcttccggt ttaataccta acacgtcggc cagtgatttc acacccgtac
    19321 ctttgttaac gcgtttatcg aggatttcga ggaagtacgg cgcacttttc agcacggtgt
    19381 atttttcttt cacttcctgc ggaatacgcg cgatggcctg gtcgaggatg gcaggttcat
    19441 caatcatcat cactttcagg aactgggtat tggggtccat tttctccgct tcgcagaaca
    19501 ccagcggaat ggtggcaacg aaagattcat gcaccgtgta gtagctgata tcacgattgg
    19561 cggtgtacag cgtggtgcgg tccagggcgt ggaaatgaga accgacttcg cgagagagtt
    19621 tttccaggaa acggtagtcg tcatagctga gagtggtttg cgccacggtg ctaccatcag
    19681 cggccttctg taccagcgcg ccgttataag taatgcagta gtcgcccggc tgttccatat
    19741 gcagctcttt caggtaattg tgcacacctg cataagggcg acccgtcgtt agcacgacat
    19801 tcacgccacg ggcgcgagct gcggcaatcg catttttaac ggcgggtgaa atggtgtgat
    19861 cgggcagcag aagggtgcca tccatatcga tagcaatgag tttgatagcc atgagttccc
    19921 caggtagatt ggttcctgac ccatgctaac gcgattccgc tcaaaaatca gtacaacacc
    19981 cgagggaaaa gggggatgca acgcgcgttg cgtgctcccc ttttgcttag cggaagagtt
    20041 tccctttcag cagttccatg cctgcggaaa gcagatcgtt attggcttgt ggtgacactt
    20101 caccttgcgg tgagagtgca tcaataatct tcggcaattg ttctgccagt aaactggaag
    20161 ctgtactggt atccacgccc agtttttgcc cgagatcgga caccgcattt gtgccgagcg
    20221 ccgattccag ttgctcgccg ctaactggtt ggttgccctg ttgattactc agccaggttg
    20281 agagaatggc acctaaaccg ccactttgca gtttttccag cagcacctga atgccgccct
    20341 gctcctcaac ccaacttaaa atcgcctgat atttccccgc atcgcctttc agaaaggcgc
    20401 cgacaacgtc atcaaaaagg cccatgataa tcacctgtaa agcgttacgt gttgactcaa
    20461 aaagtatagt tctgtgggat gataattgcg gattgcagga ataaaaaggg cggagatgat
    20521 ctccgccctt ttcttatagc ttcttgccgg atgcggcgtg aacgccttat ccggcctaca
    20581 aaaacatgca aattcaatat attgcaggag tctcgtaggc ctgataagcg tagcgcatca
    20641 ggcacgctcg cattgttaac gccattaaat atcaatattc gccgctttca gagcgttctc
    20701 ttcaataaac gcacggcgcg gttcaacggc gtcgcccata agcgtagtga acaactggtc
    20761 ggcagcaatc gcatctttaa cggtaacgcg cagcatacga cggctttccg ggtccatggt
    20821 ggtttcccac agctgttccg ggttcatctc gcccagacct ttataacgct ggatggagag
    20881 gccgcgacgg gactctttca ccagccagtc cagcgcctgc tcgaagctgg ctaccggctg
    20941 acgacgttcg ccacgttcga taaatgcatc ttcttccagc aagccacgca gtttctcacc
    21001 cagcgtgcag atacgacgat attcgccgcc agtgataaat tcgtgatcca gcggatagtc
    21061 agtatccaca ccgtgggtac gcacgcgaac aatcggctcg aacaggtttt gttcggcatt
    21121 ggtgtggaca tcaaacttcc actggctgcc gtgctgttct ttgtcgttca gttcgctgac
    21181 cagcgcgttc acccagcggg taacggtctg ctcatcagag aggtcggctt ccgtcagcgt
    21241 cggctgatag ataagctctt tcagcattgc tttcggataa cgacgctcca tgcggttgat
    21301 cattttctgc gtcgcgttgt actcagacac cagtttctct aacgcttcgc cagccaatgc
    21361 cggtgcactg gcgttggtgt gcagcgttgc gccatccagc gcgatggaga tctggtactg
    21421 atccatcgct tcgtcgtctt taatgtactg ttcctgcttg cctttcttca ctttgtacag
    21481 cggcggctga gcgatgtaga cgtgaccgcg ttcaacgatt tccggcatct gacgatagaa
    21541 gaaggtcaac agcagcgtac gaatgtgcga gccgtcgacg tccgcatcgg tcatgatgat
    21601 gatgctgtga taacgcagtt tgtccgggtt gtactcgtca cgaccgatac cacaaccaag
    21661 cgcggtgata agcgtcgcca cttcctggga agagagcatc ttatcgaagc gcgctttctc
    21721 gacgttgagg attttaccct tcagcggcag aatcgcctgg ttcttgcggt tacgcccctg
    21781 cttcgcagag ccgcccgcgg agtccccttc caccaggtac agttcagaga gcgccggatc
    21841 gcgttcctgg cagtctgcca gtttgcccgg caggcccgct aagtcgagcg cacctttacg
    21901 gcgggtcatt tcacgcgcgc gacgcgccgc ttcacgggca cgggcagcat cgataatttt
    21961 gccaaccacg attttcgcgt cggttgggtt ttccagcagg tattctgcca gcagttcgtt
    22021 catctgctgc tcaaccgccg atttcacctc agaagaaacc agtttgtctt tggtctggga
    22081 ggagaatttc gggtccggca ctttcacgga aacgaccgca atcaggcctt cacgcgcatc
    22141 gtcaccggtg gcgctgactt tggctttttt gctgtagcct tctttgtcca tgtaggcgtt
    22201 cagggtacgg gtcatcgccg cacggaagcc tgccaggtga gtaccgccgt cacgctgcgg
    22261 aatgttgttg gtaaagcagt agatgttttc ctggaagcca tcgttccact gcaacgccac
    22321 ttcgacgcca ataccgtctt tctcggtgga gaagtagaag atattcgggt ggatcggcgt
    22381 tttgttcttg ttcagatatt caacgaacgc cttgatgccg ccttcatagt ggaagtggtc
    22441 ttctttgccg tcgcgcttgt cgcgcagacg aatggaaacg ccggagttga ggaacgacaa
    22501 ctcacgcaga cgtttcgcca gaatttcata ttcgaactcg gtcacattgg tgaaggtttc
    22561 gaggcttggc cagaaacgca ccatggtgcc ggttttttca gtctcgccgg taaccgccag
    22621 cggggcctgc ggtacaccgt gttcgtagat ctgacggtga attttaccct cgcgctggat
    22681 aaccagctcc agtttttgcg acagggcgtt tactaccgaa acaccaacgc cgtgcagacc
    22741 gccggacact ttataggagt tatcgtcaaa tttaccgcct gcgtgcagaa cggtcatgat
    22801 cacttccgcc gccgatacgc cctcttccgg gtgaataccg gtcggaatgc cgcgcccgtc
    22861 atcctgtaca gagacagagt tatcggcgtg aatggtgacg ataatttctt tacagtgacc
    22921 cgcgagcgct tcgtcgatag cgttatctac cacctcgaat accatgtggt gcagaccggt
    22981 gccgtcatcc gtgtcgccga tatacatacc cgggcgctta cgcaccgcat ccagcccttt
    23041 caggactttg atactggagg agtcataaga attcgacatc aacgtttctc gctcatttat
    23101 acttgggtta atccgttatt ttaccctttt ccacggtaaa catcttcgaa ttttcgtccg
    23161 acatgtctat aacgtgttca gcactgatcg cgctgacaaa gacctgtgat tgcgtcgctt
    23221 ttaagcggct ggcaagcagc ccgcgacgct catcatcaag ctcagaggca aaatcatcta
    23281 tcaggtagag acaccgccgc ccgctttcac gggtgaggaa ctctccttgc gccagacgta
    23341 aggcgcacat caacagctta agctgcccac gcgataaggt atcttccacc ggcgcaccgt
    23401 cggcgcgaat gcgtaagtcc gctttatgcg ggccgtgcgc ggtgtaggtt agctggcgat
    23461 cgcgttcaaa attacgttcc agcacctcag cgtactctgt ctctttctcc cagccgcgct
    23521 ggaaagagaa agtcagagaa aactcaggga gaaattgctt acaggtatcg gccatatcag
    23581 ccgcgatacc ggcgctatac tccgcgcgcc aggtgcttat ttgctccgcc agcggaatca
    23641 gttctttatc ccacgggcgt agctgttcgt aacgtgtcac ctggcgcagc gccgcattgc
    23701 gctgcttgag caatcgcttg agattgctcc aggcggtgaa aaatccgggt tcgttgtgaa
    23761 agcatcccca gtcgaggaat gctcttctgt atttggggcc gccgttgagt aaagtaaacc
    23821 cttctggcgt tatcaactgc attggcatca ggtgcgccag ttccgcgacc ttatgcccgt
    23881 ctgtaccgtc gatgcggact ttgctgtcgc cctgtttgtc tttggttaag ccaatcgccg
    23941 tctcgcgctc ttcgccctgt aatcgcccgt ggagaacaaa cgcctcctgc tcgtggcgaa
    24001 tgacgcgacc aatctgcaaa ctgcgaaacg cccgaccatg gccgagcgta tagatggctt
    24061 ccagcacgct ggttttgcca ctaccgttgg cacctaccag aaagttaaag ccgggagata
    24121 aagcgagatc cgcggtttcg atattgcgga aatcgcggat caacaagcgg gtgagggaca
    24181 ttacagtctc attggcatga caacataggc agccgactgt gatgcggcat cttcaatctg
    24241 cacgctggaa accgaatcgg tcagcatcat gcggacgttt tcgcatttca gtgcgttcag
    24301 aacatccagc acatagctga cgttgaagcc gatttccatc tccgcaccgc tataggtaac
    24361 gtcgaggatc tcttccgctt cttcctgttc tgggttgttg gcggtgattt tcagctggtt
    24421 ttcgctgaca tacagacgca cgccgcggaa tttctcgtta gagagaattg ccgcacgggc
    24481 aaacgcctgc ttgagcagat cacagccagc ttccagatgc ttgtccgggt tcttcggcag
    24541 aacgcgacgg taatccggga agcgaccatc caccagtttc gaggtgaaga taaagtcgcc
    24601 aacgtgggcg cgaatgttgt tgctgccaat ctgcacgcgc agcggattgt cgccgccgtc
    24661 gagcatacgc atcagttcaa tcacgccttt acgcggcacg atcaccgaat ggcttggcaa
    24721 agattgacca attggcattg aacagactgc cagacggtgg ccgtcggtcg ccacggtgcg
    24781 cagttcttca ccttcggttt caaacagcat accgtttaag taatagcgaa cgtcctgatg
    24841 cgccatagaa aactgggtcg cttcaatcag acgcttcatc gtcgcctgcg gcagggtaaa
    24901 ttcgacttcg ctctgccagt catcgaggtt cgggaaatct gccgctggca gggtagacag
    24961 cgaaaaacgg ctacgcccgg agcgcaccag catccgctca ccttccagct gcacggcaat
    25021 ttccgcgcct tcaggcagac cacggcagat atcaaagaat ttgcgcgccg gaacggtcgt
    25081 cgcccctggt tcgtgcggct gaaccagcgc aacgcgtgcc accatttcca tctcgagatc
    25141 agtaccggtc agcgacaacg taccgtcagc aacctgtaac agcagattac cgagaatcgg
    25201 tagcgtagga cgaccaccta acggaccgct cacctgttgt agcggtttta ataaatgctc
    25261 acgttctacg gtaaatttca taggtttacg atgacaatgt tctgattaaa ttagaaaaat
    25321 cttctttgat atcgtggctc tcttcacgca actgctcgat cttccggcag gcatgaagca
    25381 ccgtcgtgtg gtcacgacca ccaaaggcat cgccaatctc cggaagactg tggttagtca
    25441 gctctttcgc cagcgccatc gccatttggc gcggacgggc caccgagcgg gatcgacgct
    25501 tggaaaggag atccgcgact ttgatcttgt agtactccgc caccgtcttc tgaatattgt
    25561 cgatggtgac cagtttttcc tgcaatgcca gcaagtcgcg cagcgcctca cgcacgaagt
    25621 cgatggtgat cgcccgtccg gtaaagttgg cattggcaat gacgcggttc agcgcgcctt
    25681 ccaactcacg tacgttagat cgtagacgct tggcgataaa aaacgccact tcgcccggca
    25741 aacgaatgtc gttttcgtcg gcctttttca tcaggatcgc cacgcgggtt tccagctctg
    25801 gcggttcgat cgccacagtc agtccccagc cgaagcggga tttcaaacga tcctcaacgc
    25861 cgttgatctc tttcggatag cgatcggagg tgagaatgat ctgttgatta ccttccagca
    25921 gggcgttgaa ggtgtggaaa aactcttcct gagatcgttc tttattagca aaaaactgaa
    25981 tatcgtcgat cagcagtgca tctacggaac ggtagtagcg tttaaactct tcgatcgcgt
    26041 tgttttgcag ggctttaacc atgtcctgaa caaagcgctc ggagtgcata taaaccactt
    26101 tggcattcgg cttgcgcgcc ataatgccgt tacccaccgc atgcagcagg tgagttttac
    26161 ccaaacccgt gccgccataa aggaacaacg ggttataagc accgccaggg ttatctgcca
    26221 cctggcgagc cgccgcgcgc gccagttggt tagatttacc ttcaacgaag ttatcaaacg
    26281 tgtgtttgac gtttacgtta gaacgatagg tcggttctgc cggagccggg acgttatccc
    26341 aacccgagcg cgtagaaggc gcagcacgtt gcggctgcgt ttgcgccacc tgtgcagggg
    26401 ccgcgacgtt gctcgtcact gccgcttgtg gcgtttgcgt caccggtttg gtgccgactt
    26461 caaaacgcag ctgtggggca tccgctccgc agaaactggt tagcagtcca ttgatattat
    26521 taaggtactt gtcccgtacc caatcgagga caaaacggtt tggcgcgtac agggccagcg
    26581 tgttatcgct cagttccgcc tgcaatgggc gtatccacat actgaattct gtggctggta
    26641 actcatcctg caatcgggca agacactgct gccaaagcga aagtgacacg gcggactcca
    26701 ctcgaacaaa agtcgataat gactaaggct gaaacattca tgattgttga cgtacgtcga
    26761 aaagaccctg catgagggtg acgcacgaac cgctgtctgc ggttatatgc ccgatcaaga
    26821 tcctgcaaaa cgatcgggac cgcggatcat agcctaaact gcgcaagaga tcttctgttt
    26881 ctcacagatt ttcccgattt atccacagga ctttccagaa ctcgctaagt gtaaacgatc
    26941 ctgccgcggg ccgggcacga tttacgccgc atattggaaa atttaatgac catagacaaa
    27001 aattggctta atcgatctaa taaagatcca ggacgatcct tgcgctttac ccatcagccc
    27061 gtataatcct ccacccggcg cgccatgctg gttttcactg gtgtgaggcc gtacattttc
    27121 cctgcgaaaa ggtgcggaaa agcgcggtaa ataaggaaag agaattgact ccggagtgta
    27181 caattattac aatccggcct ctttaatcac ccatggcttc ggtgtccatc gtttcatttt
    27241 tcggcggata tccaataaag ccattgaatt tattcaagtt taggtagaaa tcgccatgaa
    27301 acgcactttt caaccgtctg tactgaagcg caaccgttct cacggcttcc gtgctcgtat
    27361 ggctactaaa aatggtcgtc aggttctggc acgtcgtcgt gctaaaggcc gcgctcgtct
    27421 gaccgtttct aagtaataaa gctaacccct gagtggttaa gctcgcattt cccagggagt
    27481 tacgcttgtt aactcccagt caattcacat tcgtcttcca gcagccacaa cgggctggca
    27541 cgccgcaaat taccattctc ggccgcctga attcgctggg gcatccccgt atcggtctta
    27601 cagtcgccaa gaaaaacgtt cgacgcgccc atgaacgcaa tcggattaaa cgtctgacgc
    27661 gtgaaagctt ccgtctgcgc caacatgaac tcccggctat ggatttcgtg gtggtggcga
    27721 aaaaaggggt tgccgacctc gataaccgtg ctctctcgga agcgttggaa aaattatggc
    27781 gccgccactg tcgcctggct cgcgggtcct gatagccctc attcgggtct atcaacgcct
    27841 gattagtccg ctactcgggc cgcattgtcg tttcactcca acctgttcaa gctacggaat
    27901 tgaggcattg cgcaggtttg gagtgataaa aggcagttgg ttgacggtga aacgcgtatt
    27961 aaaatgccac cctttacacc ctggtggtga cgatcccgtc ccgcccggac catttgatac
    28021 cagagaacac taacgatgga ttcgcaacgc aatcttttag tcatcgcttt gctgttcgtg
    28081 tctttcatga tctggcaagc ctgggagcag gataaaaacc cgcaacctca ggcccaacag
    28141 accacgcaga caacgaccac cgcagcgggt agcgccgccg accagggcgt accggccagt
    28201 ggccagggga aactgatctc ggttaagacc gacgtgcttg atctgaccat caacacccgt
    28261 ggtggtgatg ttgagcaagc tctgctgcct gcttacccga aagagctgaa ctctacccag
    28321 ccgttccagc tgctggaaac ttcaccgcag tttatttatc aggcacagag cggtctgacc
    28381 ggtcgtgatg gcccggataa cccggctaac ggcccgcgtc cgctgtataa cgttgaaaag
    28441 gacgcttatg tgctggctga aggtcaaaac gaactgcagg tgccgatgac gtataccgac
    28501 gcggcaggca acacgtttac caaaacgttt gtcctgaaac gtggtgatta cgctgtcaac
    28561 gtcaactaca acgtgcagaa cgctggcgag aaaccgctgg aaatctcctc gtttggtcag
    28621 ttgaagcaat ccatcactct gccaccgcat ctcgataccg gaagcagcaa cttcgcactg
    28681 cacaccttcc gtggcgcggc gtactccacg cctgacgaga agtacgagaa atacaagttc
    28741 gataccattg ccgataacga aaacctgaac atctcttcga aaggtggttg ggtggcgatg
    28801 ctgcaacagt atttcgcgac ggcatggatc ccgcataacg acggtaccaa caacttctat
    28861 accgctaatc tgggtaacgg catcgccgct atcggctata aatctcagcc ggtactggtt
    28921 cagcctggtc agactggcgc gatgaacagc accctgtggg ttggcccgga aatccaggac
    28981 aaaatggcag ctgttgctcc gcacctggat ctgaccgttg attacggttg gttgtggttc
    29041 atttctcagc cgctgttcaa actgctgaaa tggatccata gctttgtggg taactggggc
    29101 ttctccatta tcatcatcac ctttatcgtt cgtggcatca tgtacccgct gaccaaagcg
    29161 cagtacacct ccatggcgaa gatgcgtatg ctgcagccga agattcaggc aatgcgtgag
    29221 cgtctgggcg atgacaaaca gcgtatcagc caggaaatga tggcgctgta caaagctgag
    29281 aaggttaacc cgctgggcgg ctgcttcccg ctgctgatcc agatgccaat cttcctggcg
    29341 ttgtactaca tgctgatggg ttccgttgaa ctgcgtcagg caccgtttgc actgtggatc
    29401 cacgacctgt cggcacagga cccgtactac atcctgccga tcctgatggg cgtaacgatg
    29461 ttcttcattc agaagatgtc gccgaccact gtgaccgacc cgatgcagca gaagatcatg
    29521 acctttatgc cggtcatctt caccgtgttc ttcctgtggt tcccgtcagg tctggtgctg
    29581 tactatatcg tcagcaacct ggtaaccatt atccagcagc agctgattta ccgtggtctg
    29641 gaaaaacgtg gcctgcatag ccgcgagaag aaaaaatcct gattcggtga gttttcgcta
    29701 aaataagggc ggtcagttga ccgccttttt tcttttcgta gggcggataa gcaccgcaca
    29761 tccgccacac aaagcaacag gaacatcatg agcgataatg acactatcgt agcccaggcc
    29821 acgcctccgg gacgtggcgg cgttggcatc ctgcgcatct ccggcttcaa agcccgtgaa
    29881 gttgccgaaa ccgtgctggg taaactgcct aagccgcgct acgccgatta tcttccgttt
    29941 aaagacgccg acggcagcgt gctcgatcag gggattgcgc tatggttccc tggcccgaac
    30001 tcgttcaccg gcgaagatgt gctggaactg caaggtcatg gcggtccggt gatcctcgac
    30061 ctgctgttaa aacgcattct gaccattccc ggcctgcgga ttgctcgccc gggtgagttt
    30121 tccgaacgcg cgtttcttaa cgataaactc gacttagccc aggccgaggc gattgcggat
    30181 cttatcgacg ccagttcgga acaggcggcc cgttcggcac ttaactcgct gcaaggcgca
    30241 ttctccgcac gggttaatca tctggtagaa gccctcaccc acttgcgcat ttacgttgaa
    30301 gcggcaattg atttccccga tgaagagatc gatttcctct ccgacggcaa aattgaagcc
    30361 cagcttaatg acgtgattgc cgatctcgat gcagtgcgtg ctgaagcacg tcagggtagt
    30421 ttgttgcgcg aagggatgaa agtggtgatt gccggacgtc ctaacgccgg taaatcgagc
    30481 ctgttaaacg cgctggctgg ccgtgaagca gcaatcgtaa ccgatatcgc cggaactacg
    30541 cgtgacgtgt tgcgtgagca tatccacatt gacggaatgc cgctgcatat catcgacacc
    30601 gccgggctac gtgaagccag cgatgaagtg gaacgtattg gtatcgagcg cgcgtggcaa
    30661 gaaattgaac aagccgaccg cgtgctgttt atggtcgatg gcaccacgac agacgccgtt
    30721 gatccagcag agatctggcc ggagtttatc gcccgtctgc cagcgaaact gccgatcacc
    30781 gtggtgcgca ataaagccga tatcaccgga gaaacgttgg ggatgagcga agtgaacggt
    30841 cacgcgttaa ttcgtctctc tgcaaggact ggtgaaggtg tggacgtgct gcgtaaccat
    30901 ctcaaacaga gcatgggctt tgacaccaac atggaaggcg gcttcctggc gcgtcgtcgt
    30961 cacctacagg cactggaaca ggcggcagag cacctgcaac agggtaaagc gcaactgtta
    31021 ggagcctggg caggcgagct gctggcggaa gaactgcgtc tggcgcagca gaacttaagc
    31081 gagatcaccg gggaatttac ttctgacgac ctgctggggc gaattttctc cagcttctgt
    31141 attggtaagt aaccgcgctt gcgaagccgc attctgactg tcagatgcgg cttcgcttca
    31201 ttgttagcac tcctgttact cctcaaccct ttttttaaac attaaaattc ttacgtaatt
    31261 tataatcttt aaaaaaagca tttaatattg cttcccgaac gattgtgatt cgattcacat
    31321 ttaaacaatt tcagaataga caaatttctt gagtattata ctgtagcctc gtgtctttcg
    31381 aggataagtg cattatgaat atcttacata tatgtgtgac ctcaaaatgg tttaatattg
    31441 acaacaagat tgtcgatcac cgcccttgat ttgcccttct gtagccatca ccagagccaa
    31501 accgattaga ttcaatgtga tctatttgtt tgctatatct taattttgcc ttttacaaag
    31561 gccatctttc gtttatttac ttgttttagt aaatgatggt gcttgcatat atatctggcg
    31621 aattaatgga tattgcaaat gtaatattca cagggatcac tgtaattaaa ataaatgaag
    31681 gattatgtaa tggaaaactt taaacatctc cctgaaccgt tccgcattcg tgttattgag
    31741 ccagtaaaac gtaccactcg cgcttatcgt gaagaggcaa ttattaaatc cggtatgaac
    31801 ccgttcctgc tggatagcga agatgtgttt atcgatttac tgaccgacag cggcaccggg
    31861 gcggtgacgc agagtatgca ggccgcgatg atgcgcggcg acgaagccta cagcggcagc
    31921 cgcagctact atgcgttagc cgagtcagta aaaaatatct ttggttatca atatactatt
    31981 ccgactcacc agggccgtgg tgcagaacaa atctatattc cggtactgat taaaaaacgc
    32041 gagcaggaaa aaggcctgga tcgcagcaaa atggtggcgt tctctaacta tttctttgat
    32101 accacgcagg gccatagcca gattaacggc tgtaccgtgc gtaacgtcta tatcaaagaa
    32161 gccttcgata ctggcgtgcg ttacgacttt aaaggtaact ttgaccttga gggattagaa
    32221 cgcggcattg aagaagttgg cccgaataac gtgccatata tcgttgcgac catcaccagt
    32281 aactctgcag gtggtcagcc ggtttcactg gcaaacttaa aagcgatgta cagcatcgcg
    32341 aagaaatacg atattccggt ggtcatggac tccgcacgct ttgctgaaaa tgcctatttc
    32401 atcaagcagc gtgaagcaga atacaaggac tggaccatcg agcagatcac ccgcgaaacc
    32461 tacaaatatg ccgatatgct ggcgatgtcc gccaagaaag atgcgatggt gccgatgggc
    32521 ggcctgctgt gcatgaaaga cgacagcttc tttgatgtgt acaccgaatg cagaaccctt
    32581 tgcgtggtgc aggaaggctt cccgacatat ggcggcctgg aaggcggcgc tatggagcgt
    32641 ctggcggtag gtctgtatga cggcatgaat ctggactggc tggcttatcg tatcgcgcag
    32701 gtgcagtatc tggtcgatgg tctggaagag attggcgttg tctgtcagca ggcgggcggt
    32761 cacgcggcat tcgttgatgc aggtaaactg ttgccgcata tcccggcaga tcagttcccg
    32821 gcacaggcgc tggcctgcga gctgtataaa gtcgccggta tccgtgcggt agaaattggc
    32881 tctttcctgt taggccgcga tccgaaaacc ggtaaacaac tgccatgccc ggctgaactg
    32941 ctgcgtttaa ccattccgcg cgcaacatat actcaaacac atatggactt cattattgaa
    33001 gcctttaaac atgtgaaaga gaacgcggcg aatattaaag ggttaacctt tacctacgaa
    33061 ccgaaagtat tgcgtcactt caccgcaaaa ctgaaagaag tttaattaaa acactcagag
    33121 tggctataag gatgttagcc actctattac cctacatcct caataacaaa aatagccttc
    33181 ctctaaaggt ggcataatga ctgatcaatc agaaaaaaaa cactctgcat tttggggtgt
    33241 tatggttata gccggtacag taattggcgg tgggatgttt gctttacctg ttgaccttgc
    33301 cggagcctgg tttttttggg gcgcttttat tcttattatc gcctggtttt caatgttaca
    33361 ttcagggcta ttattattag aagcaaactt aaattatccc gtcggatcca gttttaacac
    33421 catcaccaaa gatttaatcg gtaacacctg gaacattatc agcggtatta ccgttgcctt
    33481 cgttctctac atcctcactt atgcctatat ctctgctaat ggtgcgatca ttagtgaaac
    33541 gatatcaatg aatttgggtt atcatgctaa tccacgtatt gtcgggatct gcactgccat
    33601 tttcgttgcc agcgtattgt ggataagttc gttagccgcc agtcgtatta cctcattgtt
    33661 ccttgggctg aagattatct cctttgtaat tgtttttggt tctttcttct tccaggtcga
    33721 ttactccatt ctgcgcgatg ccaccagcac cacggcggga acatcttatt tcccgtatat
    33781 ctttatggct ttgccggtgt gtctggcgtc atttggtttc cacggcaata ttcccagcct
    33841 gattatttgc tatggcaaac gcaaagataa gctaatcaaa agcgtggtct tcggttcgct
    33901 gctggcgctg gtgatttatc tcttctggct ctattgcacg atggggaata tcccgcgaga
    33961 aagctttaag gcgataatct cctcaggcgg caacgttgat tcgctggtga aatcgttcct
    34021 cggcaccaaa cagcatggca ttatcgagtt ttgcctgctg gtgttctcca acttagctgt
    34081 tgccagttcg ttctttggtg tcacgctggg attgttcgat tatctggcgg atctgtttaa
    34141 gattgataac tcccacgccg ggcgtttcaa aaccgtgctg ttaaccttcc tgccacctgc
    34201 gttgttgtat ctgatcttcc cgaacggctt catttacggg atcggcggtg ccggactgtg
    34261 tgccaccatc tgggcggtca ttatccccgc ggtgctggca atcaaagctc gcaagaagtt
    34321 tcccaatcag atgttcacgg tctggggcgg caatcttatt ccggcgattg tcattctctt
    34381 tggtataacc gtaattttat gctggttcgg taacgtcttt aacgtgttac ctaaatttgg
    34441 ctaaatcctt caagaagcca gccattcgct ggcttcttgc ctctcaggaa atcacttatg
    34501 tccaaatggc aactcgcctg atcctccttc accacgtatg ctttgcgtca ccttactatc
    34561 aggacgcttt agcccatgtc ccgctttttg atttgtagtt ttgccctggt tttactttat
    34621 cccgcaggga ttgatatgta cctcgttggt ttaccgcgca tcgccgccga tctcaatgcc
    34681 agcgaagcgc agttgcatat tgcgttctcc gtttatctgg cggggatggc ggctgcgatg
    34741 ttatttgccg gtaaagtggc cgatcgttca gggagaaagc ctgtcgccat acccggcgcg
    34801 gcgctattta ttatcgcctc ggtgttctgt tcactggctg aaaccagcgc gttatttctt
    34861 gcagggcgat ttctacaggg actgggcgcg ggctgttgtt acgtagtggc gttcgccatt
    34921 ttgcgcgaca cgctggatga tcgacgtcgg gctaaagtgc tgtcgttact caacggcatt
    34981 acctgcatca ttccggtgtt agcgccggtg ctcggacacc tgattatgct taaattccct
    35041 tggcagagtc tgttctggac gatggcaacg atgggcatcg cggtactgat gttgtctttg
    35101 tttattttaa aagaaacgcg ccctgcggcc cccacaactt cggacaaacc acgagaaaat
    35161 agcgagtcac tgcttaatcg gtttttcctc agccgtgttg ttatcaccac cctcagcgtt
    35221 tcggtgatcc tcaccttcgt caacacgtcg ccagtattgc tgatggaaat catgggtttt
    35281 gagcgcggag aatacgccac cattatggcg ttgaccgctg gcgtcagcat gaccgtttca
    35341 ttctccacgc cgtttgcgct gggaattttt aagccacgta cgttgatgat cacctcacag
    35401 gtgttattcc tggcggcggg gatcaccctt gccgtttcac cttcccatgc ggtttcgctg
    35461 tttggtatca cgctgatttg cgccggtttc tcggtaggtt ttggtgtggc gatgagtcag
    35521 gcgttagggc cgttttcatt acgcgcgggc gtagccagct cgaccttagg tattgcgcaa
    35581 gtttgcggtt cgtcactgtg gatttggctg gcggcggtgg ttggtatcgg cgcatggaat
    35641 atgctgatcg ggattctgat tgcctgtagc atagtgagcc tgttgctgat tatgttcgtc
    35701 gcgcctggac gcccggttgc cgctcatgaa gaaatccatc accacgcttg atctcaatct
    35761 gctgctctgt ctgcaactgc tgatgcagga gcgcagcgtg accaaagcgg cgaagcggat
    35821 gaacgtgacc ccttcggcgg tgagtaagtc gctggcaaag ttaagagcgt ggtttgacga
    35881 cccgctcttt gtgaactcac cgctggggct gtcgcccaca ccgctaatgg tcagcatgga
    35941 gcaaaatctg gcggagtgga tgcaaatgag caacctgctg ctggataaac cgcaccacca
    36001 gacaccgcgc ggcctgaagt ttgagctggc agcagaatca ccgctaatga tgatcatgct
    36061 taatgcgctg tcgaaacgga tctaccaacg ttacccgcag gcgaccatca aattacgtaa
    36121 ctgggattac gattccttag atgccattac tcgtggtgaa gtggatattg gtttttccgg
    36181 tcgcgaaagt catccccgtt cgcgggagct gttaagctcg ctaccgttag ccattgatta
    36241 tgaagtgctg tttagtgatg tgccctgcgt ctggttacgc aaagatcatc cggcactgca
    36301 tgaagcatgg aatctggaca cctttttacg ttatccgcat atcagcattt gctgggaaca
    36361 gagcgatacc tgggcgctgg acaatgtgtt acaggagctg gggcgcgaac gcacgattgc
    36421 tatgagcctg ccggaattcg agcagtcact gtttatggca gcacagcccg acaatctgct
    36481 actggcgacc gcaccgcgct actgtcagta ctacaatcaa ctccaccaac tgccgttggt
    36541 tgctcttcct ctcccgtttg acgaaagcca gcaaaaaaag ctggaagtcc cttttaccct
    36601 gctgtggcat aaacggaaca gccataatcc gaagatcgtc tggttacggg aaaccattaa
    36661 aaatctttac gcgtcgatgg cataaccgaa tcgtatgaaa cgcgacccaa tttcacaata
    36721 aaatgtaaaa aagttgtaat aagcttgtct gaatcgaact tttagccgct ttagtctgtc
    36781 catcattcca gtaaatgatt actcttgtat tcataatgga ccattaagca tggagcgaaa
    36841 aatggcgact cactttgccc gagggatttt aacggaagga catctgattt ctgttcgtct
    36901 cccctcccag tgtcatcaag aagcccgaaa cattccccct catcgtcaaa gccgttttct
    36961 ggcgtccaga ggtttactcg cagaactgat gttcatgctg tatggcattg gcgaattgcc
    37021 ggaaatcgtc accctgccga aaggaaaacc ggttttcagc gataaaaatt tgccttcgtt
    37081 ttctatttcc tatgccggga atatggtcgg cgtggcgtta acaaccgaag gtgaatgtgg
    37141 cctcgatatg gaactacagc gtgcgacgcg cgggtttcat agcccacacg cgcccgataa
    37201 ccacaccttt tccagcaatg aatcgctatg gatcagtaaa caaaacgatc ctaacgaagc
    37261 gcgggcgcag ctcatcacgc tgcgccgaag cgtgctaaaa ctaaccggtg atgttttgaa
    37321 tgacgatccg cgcgatctgc aactactgcc cattgccgga cgcctgaaat gtgctcatgt
    37381 aaatcatgta gaagcgttat gcgacgcgga ggacgtgctg gtgtggtccg tggcggtcac
    37441 gcccacgatt gaaaagctca gtgtctggga gttagatggc aaacatggct ggaaaagcct
    37501 gcccgatatt cacagccgcg ccaacaatcc taccagccgg atgatgcgtt ttgcccagct
    37561 ctctaccgtg aaggcttttt cgccaaattg atagacaacc acaggagtca tcatgtctga
    37621 aaaattgcag gtggttacgt tactagggag cctgcgcaaa ggctcattta atggcatggt
    37681 tgcacgtacc ctgccgaaaa ttgctccggc gagcatggaa atcaatgcgt taccttccat
    37741 tgccgacatt cccctgtatg acgctgacgt acagcaggaa gacggttttc cagcaacagt
    37801 tgaagctctg gcggaacaga tccgtcaggc tgacggtgtg gtgatcgtca cgccggaata
    37861 taactactcg gtaccgggtg ggctgaaaaa tgccatcgac tggctttccc gcctgccgga
    37921 tcaaccgctg gctggtaaac cggtattgat tcagaccagc tcaatgggcg tgattggcgg
    37981 cgcgcgctgt cagtatcacc tgcgccagat tctggttttc ctcgatgcaa tggtgatgaa
    38041 caagccggaa tttatgggcg gcgtgattca gaacaaagtt gatccgcaaa ccggggaagt
    38101 gattgatcag ggtacgctgg accacctgac cgggcaattg accgcatttg gtgagtttat
    38161 tcagcgagtt aagatctaaa taaaaaaccc gccagcaatc atgcatggcg ggtttttaac
    38221 gcgctatcga ttttagtgag cgtcgataaa gacaatcttc aggataaaca gcagcgcaac
    38281 gatgattacg cacgggctaa ggtcacgcag acgtccggta ccaatcttca tcacgcagta
    38341 ggagataaag cccagcgcga taccttcagt aatcgagaag ctgaacggca tcatcacagc
    38401 ggtaataaac gccggaacag attcagtaag atcctgccag ttcacgcgag caagacttga
    38461 ggtcatcaac acgccaacgt aaatcagcgc accagctgca gcgtagcctg gcaccatccc
    38521 cgccagcggc gacaggaaga taaccagcag gaacagcaga ccaacaacca ctgccgtcag
    38581 accggtacga ccgccaacgg aaacgccgga agaagattca atatacgccg taacagaaga
    38641 agtaccgata aacgaaccgg tcaccgaaga gatactgtcg acatacagcg cctgcttcat
    38701 gcgcgggaat ttacctttct catcagccag acctgcttta tcggtcacgc caatcagcgt
    38761 accggaggag tcaaacaagt tgaccagcat gaaagagaaa atcacccctg ccagcccaag
    38821 gttaaacgac ccggctaaat ctacatgacc cacgacactc attacgctcg gcggcgcaga
    38881 aacgatgcca ttatagtgga catcacccag catccagccc agcagcgttg tcaccacgat
    38941 ggaaaccagc accgccgcgt gaatattgcg cgaggccaga atggcgatga tgaagaagcc
    39001 gaggatcccc agcagtacgc tgtgagaagt cagattaccg atgctcacca gcgtttccgg
    39061 gttagcgaca atcacacctg cattcttcag tcccatcatg ccaataaaca ggccgatacc
    39121 gctggtaatg cccacacgca gactcaccgg aatgttggct atcatccagt agcgaacgcg
    39181 gaaaatcgtc agtaacagca gaccaatcgc gccccagaag attgcgccca tcccgacctg
    39241 ccacggcaag cccatcgcct gcacaaccac aaaagcgaag aacgcattca ggcccatagc
    39301 gggtgccagt gcaactggca ggttagcaaa cagtcccatc ataatactgc cgaatgcagc
    39361 aatcagacag gtagtaacga agacggcgct ggtatccatg ccagcaacgc caagaatttg
    39421 cgggttaaca aaaacgatgt aaaccatcgt caggaaggtg gtaaaaccgg cgatcacttc
    39481 ggtccgtgcc gtcgtgccat gttcgcgcag tttaaacacg cgttccagca tcccctggcc
    39541 agaagcctgg gttgtgtgtt gttgactcat tatctatttc cgaacaagga gggaaaatcc
    39601 gtcgctatcg tataccaaaa tgagacaata tggacggctg aagataactt tttttcattg
    39661 tatttgctta tacggcaacg attgcgtgat gcgaattggc atgacgaaaa cgttaaacta
    39721 acgataaagg aaagacatgt cccagataga agcggtattt ttcgactgcg acggtacgct
    39781 ggtcgatagc gaggtcattt gctctcgcgc ctatgtcacg atgttcaggg aatttggcat
    39841 tcacgttgac ctggaagaga tttttacccg ttttaaaggc gtaaaactct acgaaatcat
    39901 cgacatcatc agtaaagaac agggcgtgac gatggtccag gccgatgccg agcacgttta
    39961 ccgcgcagaa gtagcacgtc tgtttgatac cgaacttgag gacatcgcag gagcgaacga
    40021 attgctggcg agcattaacg tgccgatgtg cgtcgtctct aacggtccgg tcagcaaaat
    40081 gcagcattcg cttggtaagc tcaacatgct gcactacttc ccggaaaaac tgttcagcgg
    40141 ctacgatatc cagcgttgga agcccgaccc ggcgttaatg ttccatgcgg caaaagcgat
    40201 gaatgtgaac gttgaaaact gcattctggt cgatgactcc agcgccggtg cgcaatcagg
    40261 aatcgatgct ggtatggaag tgttttactt ctgcgctgac ccgcacaata agccgatcgt
    40321 tcatccgaaa gtcaccacct ttacccatct ttcgcaatta ccggaactgt ggaaggcgcg
    40381 tggttgggat attacggcat agttattcac actcccttca cttactccac ttaaattggc
    40441 acgcaaaggt aagtaaaggg agtttgatat gtctgtttca cgtcgggtaa ttcatcacgg
    40501 actgtatttt gcggttttag ggccgttaat tggtgttctg tttcttgtcc tctacatatt
    40561 cttcgcaaaa gaaccgctga ttcttttggt aataatacag gtacttccat tgtttctctt
    40621 aatgtcgata actacgggag ctattcctgc aatgttaact ggcgtaatgg tcgcctgtct
    40681 gccggaaaag atcgggtcac agaaacgtta tcgttgtctg gttggcggca taggtggagt
    40741 cgttatcacc gagatctatt gcgctgttat cgtacatatt aaggacatgg cttcctcagc
    40801 gttgtttgaa aacattcttt ctggtgaaaa tctcgttgtc cgcatcatcc cagcattgct
    40861 ggcaggcgta gtgatgagca gaatcattac acatctaccc ggattggata tttcatgtcc
    40921 tgaaacagac tctttaagct aagcggaatg ctttatctat ttgtcattaa atgttggaga
    40981 acaggccgga gcgtaattca cacatccggc cttattttta agcttaaccg agcgctaatt
    41041 ctgcggcagc ggctttatca gcgatgacta tcagcgaagg gtgcaactgc aatacggaag
    41101 caggaacatc ttcggtaacc ggaccttgca gaacattttt cagtgcctgc gctttacccg
    41161 cgccactgac aattatcagc aagtttttcg ccgccatgat gcttttcggc cccatcgtca
    41221 cgtagctgtc aggcactaat gaaaagtcgc cacccaactc gccatgcgcc acaatatcaa
    41281 ccatctctcc ctggatcggg aactccacgg tttgctcatg gaaatgggtc gtatttggca
    41341 gattgccaca aaaatgacca tctacaccta atcccaacac caccaaatcc aggccgcctt
    41401 cacgcgccag tttctgatca tgctcgcggt agttatcaat agtgagcttc tggatattct
    41461 cttctttgat ccctgcaggg gtgaaaaaca gattatgcag attggtaatc gttacgcctt
    41521 ccccttcttt accgcgaaat ggaatttcat cgaaattgta gaaatagcag ttatcgtacc
    41581 agggcttacc tttaaccaga gtgatgagat attcgtacat gcctttgggc gtgctcccgg
    41641 cggtaattgc caggttaacg cgacgcatct ttgacatata accaagcaga tgatgtgccg
    41701 cgacacggct catttcctgg taatcttcgg taatgatcaa tttcatctta atgccttata
    41761 ttatttgaat aatttctgta caaaggctgc ataggctggt cgccagacat ccatttcatg
    41821 gttcagaccg ggatattcct ggtaatcaaa cttaattttt ttctgctcaa gctcagtttt
    41881 cagcccggcg atatccttgc cggttacgac atctttatcg cccacgacca cagtaaaatt
    41941 acgtagttgc tggttaatag ctgccggctc gttcagccgg gcagcgaccc cttcatctgg
    42001 tacggtcgtc gtggtaacac cactgaatgt ggccagccag ccaaagcttt ccagatgatt
    42061 cattcccgat accagtgcct gatacccacc ttgtgaaagc cctgccagtg cgcgaccatc
    42121 ggcatcttta cggacattaa aacgcttgct aatcagcggg ataatatcgt tcatcaattc
    42181 gcgatctgcc gctttagcat ttaacggata aaagacttta cgtctctcct gaggaacgaa
    42241 atcttcggga ataatgccct tcgcatcggt ttcggtatcc gggatcacca ccagcatcgg
    42301 tttaattttc ccttcagcca gcaggttatc catgatttgc gggatacgcc cctgatcaat
    42361 agcggaacgt ccggtatcac caaagccgtg atagaaatag agcactggca aaggctcgcc
    42421 tatgccggtg tatcctggcg gggtccagac atacatctga cgttcggatt gcaatgcgtt
    42481 ggagtggtaa gttatggcga tcaaatcacc atgcacaaca gaacgtgtat ccagataact
    42541 gcccggtacc agaatcatac tggagttaac ctggcgctga ggcttagtca ttgctgtacc
    42601 tgtatcaatg ctgcgtacac catcaacatt gaaaaaatac tcgtacaggt tgcctttcag
    42661 agcaggcgtg cgccacgacc agactcccgc ttcgtctttg gtcatcgggt gaatattgtc
    42721 cggaaccgga acacccacca ctacagagac attttttgcc ccaggggcaa agtagcgaaa
    42781 ggtcacgctg ttatcggtat tgacctgagt cacatactgt ttcaccggaa tagtgggtgc
    42841 cgggctggct ggcatatccg ctgctatggc ccattgagca gaaataccgc tggcaatagc
    42901 cagcgttaaa gcagcaattt ttatattcat cgtaatattc cttttattaa gttccattac
    42961 caccagattt cagcctgggc accgacagca aactggtcgt ctttattgtc ttcaaaggtg
    43021 aagccatcca gttcgttttc cagagctttg atatatgtag cgtagaaacg aatttccggg
    43081 cgcgaggtca acatactggt attgacttta aaggtatgga agagcgtggt tttataacca
    43141 gactcattat atttattgct attcgcatct ttgttttgct gggtgaaata gcccagttca
    43201 acacctgtct ggttatattg gtcccaaata taggccgggc gaacaaccgc acgaatagat
    43261 tcgaaatcag aatgtgcgcc tgtttcgtag ctataaatat cgttaccgaa ggagtaaaca
    43321 atcgcgttag ccacaatgaa atgatcgccg atataagctt cgccctgcga agtcagacga
    43381 actgcagttc cgcctgtgtg atcaccataa taacgaccat taaaggtagt aaatggactt
    43441 gcaccagcat aacgaccaaa gttactggcg atagagttat tcgcaaccag gaaggagaat
    43501 tcgttgaagc cacccttatc aaatttctgc gttaaagatg taccaaacat ccaggtatct
    43561 ttccagtcat aatacccgtt attatcctga ttatcttttt ccgatgcgct ttcatttgct
    43621 gagacataac gaccgctcac cattaaggtg gctttatccc ataacgggat atctttatag
    43681 cgtaaatcaa tggtattggt attaatctgc tgtttgtttt gcaggctgcg atcgtaatca
    43741 tcgacatctt cacgaaccag cgcgatatca attttacccg gaccgacttt ccagttttcc
    43801 agacctacac ctgcggcagc atcggtacgc tgcgttttcc agtcaagcat ctggatttca
    43861 attttcggcg caccgtgttt acccacccag aaatcagcct ctggcgcaaa gggcaggaaa
    43921 cctttggtgg taacgtacat atcagagaac tgcataaagt tctcaccacc ggcgttatcg
    43981 ccaaaccagc cagtagagta ctgctgacca acgttaccat ccatcatcac gatggcatca
    44041 acccgtttgc cgttttcgtt gtagacacgt tgttttaact gcaaatcaaa ccagccggag
    44101 tattcgttac caaagcggcc cagagaacca attgcccatg atttaggtga accatgagag
    44161 gctgttcccc aaccagaacg gtagtagccg ttgtaactaa agccgatttc atctttcaca
    44221 aatttactga aatctttcag agtcatggaa gaataaatcg agcctgtctc actggcattt
    44281 ttctcttcat ttttaaccag tacagcatca ggctttgcgg cacgactggt cgggaatgga
    44341 ttggcggcat acccttgatc attcgtactt acgctacgat tcaccgtcgc tggcgtatac
    44401 tttttcttct cttcatcttt atattttttc agttcgcttt gcgtttctct taacgccttt
    44461 tctaatagct caaggcgttg ttccactgtt aatgattcag ccaatgattg tgcggaaaag
    44521 gctaacggtg ccattaataa gatggcagag gtaataagat ttcgtctaaa cataatatcc
    44581 ctttatggtg caaagaaaga attaacgcat cgcatccaga ctgttctgaa tgcgacgata
    44641 attaaggtgc tttaatggtt atttttttaa tgacagcccc cgagttttga tcacttctgc
    44701 ataccatccg aagcttttct tgcgcgttct tgtgaggctt ccttcaccat tatcatcacg
    44761 atccacataa ataaagccgt agcgcttaga catttgtgaa tgagatgcac tgactaaatc
    44821 aattggcccc caactggtgt accccataat atccacacca tcggcaatcg cttcatttac
    44881 ctgtaccagg tgatcgttta aataggcaat tcgataatcg tcctgtatcg aaccatccac
    44941 ttcaacgctg tcttttgcgc ctaatccgtt ctcgacaata aataacggtt tttgataacg
    45001 atcccaaagc gtatttaaca gaatccgtaa tccaaccgga tcaatttgcc acccccactc
    45061 tgaacttttc agatgcggat ttgggatcat attcagtatg ttgccctgcg catttttatt
    45121 aatgctttcg tcgtgggaaa cacaaccggt catgtaataa ctaaaagaga tgaaatctac
    45181 ggtatgtttt aaatcttctg cgtcactttc agtcatctca atggtgatat tgtggtcgcg
    45241 gaagaaacgc tgcatatagc cgggatactg accacgcgcc tgaacatcac caaagaacat
    45301 ccagcgccgg ttctcttcca tggcctgcaa catatcctgt ggctggcagg tgagggggta
    45361 aaccagccca ccgagaagca tattgccaat tttcgcttcg gggatcaggc tatgacaggc
    45421 tttaactgcc cgcgcactgg caaccagttg atgatggatc gcctgataaa cttccgcctc
    45481 gccactctct tctgccagcc ccactcccgt gaatggcgcg tgtaatgaca tgttgatttc
    45541 attaaatgtc agccataaca caactttatg ttggtagcgg gtgaagaccg tgcgggcgta
    45601 atgctcgaag tgatcgatga ccgcccgatt agcccaaccg ccgtagtttt tcaccagccc
    45661 atatggcatt tcgtaatggg ataacgttac cagcggcttg atccccgcct gcgccatttc
    45721 atcaaacagc cgatcgtaaa acgctaaccc cgcttcattc ggttcggctt cgtcgccctg
    45781 agggaaaatt cgcgcccagg caatggaaat acgcagacag gtgaagccca tctcggcaaa
    45841 taacgcgata tcttccgggt aacggtgata aaaatcgatg gcgacatctt tgatattctc
    45901 tttccccagg atgcgcggtt ccatttttcc cattacgcca tgaggctgta aatctgaggt
    45961 cgtgatccct ttgccatctt cctgccaggc gccttccacc tgattggcag ctgttgcacc
    46021 accccaaaga aatgtttctg gaaatgcttt cataattaac tccttttatc gttagcgaat
    46081 gatggataac agcggctcac cagcgtttat ctgcgccgtg ccgtggggta atacgtccgt
    46141 aaaatcatcg ctattactga ttaataccgg cgtcgtcaga tcaaatccgg cctcgcgaat
    46201 agcagggata tcaaaagaaa tcagccgatc gcctgtattg accttgtcac ccacgttgac
    46261 gtgagcggaa aagaatttgc cgtccagttt tacggtatcg ataccgacat gaatcaggat
    46321 ctccacacca tcatctgact caatgccaat ggcgtgtaat gtggcgaaca acgaagcaat
    46381 tcgacctgcc accggagaac gcacttcacc aaccgagggc ataatggcaa tacctttacc
    46441 caacaggccg ctggcaaacg tggtatcagc gacgtgaatg agcggcacaa tctctcccgt
    46501 catcggtgaa cagataccgc cctgctcagg tgtaataacc tctggtgttt tctcttgctg
    46561 ggcaacttgc gctgactgac tcttagcggt gatgaaatga aacatcaccg taccgacaaa
    46621 tgcgcaaccg atggcaatga caccgccaat aacgctggcc catacggtga aatcaatccc
    46681 cgttgacggg atggtttgca tgaaggtgaa aatacctggc aaaccaaagg agtaaacttt
    46741 cgtttgcgcg tagccaatga tggtggcccc caatgcccca cttatgcagg cgataacgaa
    46801 ggggtactta cgcggcagat tgacgccata taccgctggt tcggtgatac caaacaaacc
    46861 tgtcaacgcc gctgatcccg ccaccacttt tttctgtgca tcgcgttcgc agaggaagac
    46921 gccgagcgcc gccccgactt gcgccataat ggcgggcatt aacagcggga tcatggtgtc
    46981 gtagcccagc acggtgaagt tattgataat taaaggtatc aaaccccaat gcagaccgaa
    47041 catcacaaag atttgccaga agccgcccat taccgcgcct gcaaatgcag gaaccgcctg
    47101 ataaagccag agataaccgg cagcaatcag ttcacttatc caggttgata gcggccccac
    47161 cagcagaaat gtgatgggcg tgatgaccat cagacatagc aagggtgtga agaaattttt
    47221 gattgccgaa ggtaaccacg cattaagtcg gcgttccaga atgctgcaca accaggcaga
    47281 aaaaataatg ggaataaccg atgacgagta attcaacaat gtgaccggaa tacccaggaa
    47341 atccaggccc agcgcatcca ctttttgccc gttctcgaag gcagtcagaa ttaatggatg
    47401 cactaacgct ccgccaatca ccatggcagt aaatggattg ccgccgaagc gtttccctgc
    47461 ggtgtagccc aggattatcg ggaagaacca aaacaaggca tcactggcgc taaataaaat
    47521 taaataagta ccactttgtt cggtcgtcca ctgaaaagtg agtgccagag ccaacatacc
    47581 tttcaggatc ccggttgccg ccatcaaacc gatcagaggc gtaaaaatac ctgaaataac
    47641 ataaacaaag cggtttaaca gattaccttt atcatcattt tccggtgcct gttgcgcttt
    47701 ttcgccaagg cctgccacgc tgttaaccgc caggaagaca tcggccacat ggttacctat
    47761 gacaacctga aactggccac cgctttccac caccataata ataccggggg tctttttcag
    47821 tacctcagct tgcgctttgc tttcatcctt taacttaaaa cgtaatcgcg ttgcacaatg
    47881 catcagactc acaatgttat ctgcgccccc gactcctgcg actattgttc tggctaactc
    47941 cgtcataact tgccctctac cgctttgcgg caaaactcca aaaaaaaacc tgaaaaaaac
    48001 ggcctgacgt gaatcaagca atttttttca ggttttgccc gcttagtgcg gtaacaatcc
    48061 tttattcagt aataatattt cagtgttctt tgcgcacgcg ctctatattt atggctaaaa
    48121 acataatctc tgcaggtgag attttacgtt gatactgcaa accaataaaa atggcgatcc
    48181 gctccgcaca ttgccatgct tgcgggtaat tttgctttac tgcttgttgt aatgattcat
    48241 cactatcgtt aatcgatgca tgttcaataa tacgccagga taaaaatttc agatgcgtaa
    48301 ccagtcgctg ataactcaag ctttcttcct ggtaattaag gctgaactga aattttatta
    48361 attgcagcat ttctcgcatt aattgcgtga cacctgcaac atcctccata tttccgctca
    48421 tttgggcact gaccagatgc atggcaataa agcccacttc atctttcggt aactgcacgc
    48481 ccaaccgttt aacaataatg gttaacgctt cttcccctag ctggaactct ttcgggtaaa
    48541 gtcgctggat atcccacagc agcgggttgg gcagtagcac gttttgctga aagcgtttaa
    48601 tcgcaaactg gcaatggtca gttagcgaga tataaatact gtcctgcaac tttcccaggc
    48661 gctcctgcgc cagagagata atacgatcac aggttgccat cacctcaaga ggcatatgac
    48721 ttaagagttc gcttaatcgc ccgttcagtt catgactgct caaggcatac tctttttcta
    48781 ttccacttga gttaattctt tcgcctgggc gtttttgaaa gccaattccg cgccccatga
    48841 cgactttttc ccgctgttga tcatcaataa ccaccacaac attattgttg agaattttgg
    48901 tgatttgcat gttcatagca aggacctttt ttataaacaa aaaaacccga cttcaccagt
    48961 attctctggt tatgtcaggt tttgcctgcg aatgcagtaa caatccagtc attaattaat
    49021 ggtttttata acgaacatcc aggttcgaaa attaatttag ttgcgtgcag agaaaatagc
    49081 aatgctctat t
//
LOCUS       DAZGQL010000024        48274 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.024, whole genome shotgun sequence.
ACCESSION   DAZGQL010000024 DAZGQL010000000
VERSION     DAZGQL010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 48274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48274
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.024"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            48..1772
                     /locus_tag="ACHKIR_001635"
     CDS             48..1772
                     /locus_tag="ACHKIR_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HFO9989386.1"
                     /translation="MSNKIFTHSLPMRYADFPTLVDALDYAALSSAGMNFYDRRCQLE
                     DQLEYQTLKARSEAGAKRLLSLNLKKGDRVALIAETSSGFVEAFFACQYAGLVAVPLA
                     IPMGVGQRDSWSAKLQGLLASCQPAAIITGDEWLPLVNAATHNNNPELHVLSHAWFKA
                     LPEADVALQRPVPNDIAYLQYTSGSTRFPRGVIITHHEVMANLRAISHDGIKLRPGDR
                     CVSWLPFYHDMGLVGFLLTPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVAPP
                     FGYELCQRRVNEKDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDDKTFMPC
                     YGLAENALAVSFSDEACGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALPEH
                     GIEIRNEADIPVAERVVGHICISGPSLMSGYFGDQVSQDEIAATGWLDTGDLGYLLDG
                     YLYVTGRIKDLIIIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCRIS
                     DEERRGQLIHALAARIQSEFGVTADIDLLPPHSIPRTSSGKPARAEAKKRYQKAYAAS
                     LHVQESLA"
     gene            1769..2683
                     /locus_tag="ACHKIR_001636"
     CDS             1769..2683
                     /locus_tag="ACHKIR_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFO9989387.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     IWVRGSLEDTHSLSELVAGASAVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYANSKHVAEQRLIAMADEITLGIFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLNAETVQTQTYELCDGVAGGYDW
                     QRVRQLAAEARCGSVRMVGIPLPLLTCLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SANNNRISEDINWFPGISLEHALRNGLF"
     gene            2715..2963
                     /locus_tag="ACHKIR_001637"
     CDS             2715..2963
                     /locus_tag="ACHKIR_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFO9989388.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            2963..4135
                     /locus_tag="ACHKIR_001638"
     CDS             2963..4135
                     /locus_tag="ACHKIR_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFO9989389.1"
                     /translation="MGLYDKYARLAGERLQFSDNGLTPFGTCIDEVYSATEGRIGNKK
                     VILAGTNNYLGLTFNHDAIREGQAALAAQGTGTTGSRMANGSYAPHLALEKEIAEFFN
                     RPTAIVFSTSYTANLGVISALADHNAVVLLDADSHASIYDACSLGGAEIIRFRHNDVN
                     DLERRMVRLGERAKEAIIIVEGIYSMLGDVAPLAEIVDIKRRLGGYLIVDEAHSFGVL
                     GATGRGLVEAVGVEDDVDIIVGTFSKSLASIGGFAVGSEAMEVLRYGSRPYIFTASPS
                     PSCIATVRSSLRTISTQPELRQKLMDNANNLYDGLQKLGYELSSHISPVVPVIIGSKE
                     EGLRIWRELISLGVYVNLILPPAAPAGITLLRCSVNAAHSREQIDAIIQAFATLKQ"
     gene            complement(4170..5249)
                     /gene="lptG"
                     /locus_tag="ACHKIR_001639"
     CDS             complement(4170..5249)
                     /gene="lptG"
                     /locus_tag="ACHKIR_001639"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFO9989390.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDDVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVDVEIFHYRDDLSLESYIYARSATIEDDKTWILHGVNHKKWLNGKETQ
                     ETSDNLPWQSAFTSMNLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYRLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(5246..6316)
                     /gene="lptF"
                     /locus_tag="ACHKIR_001640"
     CDS             complement(5246..6316)
                     /gene="lptF"
                     /locus_tag="ACHKIR_001640"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFO9989391.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRARSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFVAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLMLVARDFSLLQKFSR"
     gene            complement(6346..6906)
                     /locus_tag="ACHKIR_001641"
     CDS             complement(6346..6906)
                     /locus_tag="ACHKIR_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFO9989392.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLRNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWHLQEQSSTILVLNKTGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            complement(6918..7742)
                     /locus_tag="ACHKIR_001642"
     CDS             complement(6918..7742)
                     /locus_tag="ACHKIR_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9989393.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVNRQELLRLICKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRVYLSSPVWSLVNYSLRHSQ
                     LESVSGFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYDAPMALDSDIRQRLELLISRFPV"
     gene            complement(7742..9091)
                     /locus_tag="ACHKIR_001643"
     CDS             complement(7742..9091)
                     /locus_tag="ACHKIR_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFO9989394.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVVAWYFDFGFAGFVIAWYASNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVVLSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            complement(9137..9895)
                     /locus_tag="ACHKIR_001644"
     CDS             complement(9137..9895)
                     /locus_tag="ACHKIR_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9989395.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERRLYQQMAQYRPELIIRLGIDIDTAISRKPDHDYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            complement(9927..10640)
                     /gene="yghS"
                     /locus_tag="ACHKIR_001645"
     CDS             complement(9927..10640)
                     /gene="yghS"
                     /locus_tag="ACHKIR_001645"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFO9989396.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAATMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPFGRYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGYLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     QPADEILQASLRAIHAALS"
     gene            10814..11506
                     /gene="yghT"
                     /locus_tag="ACHKIR_001646"
     CDS             10814..11506
                     /gene="yghT"
                     /locus_tag="ACHKIR_001646"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFO9989397.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIEKNEVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEKVLVDAEK
                     AITDFMTARGYH"
     gene            complement(11555..13054)
                     /gene="pitB"
                     /locus_tag="ACHKIR_001647"
     CDS             complement(11555..13054)
                     /gene="pitB"
                     /locus_tag="ACHKIR_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HFO9989398.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRFWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(13346..15205)
                     /gene="gss"
                     /locus_tag="ACHKIR_001648"
     CDS             complement(13346..15205)
                     /gene="gss"
                     /locus_tag="ACHKIR_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HFO9989399.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFSVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKE"
     gene            15410..16276
                     /gene="yghU"
                     /locus_tag="ACHKIR_001649"
     CDS             15410..16276
                     /gene="yghU"
                     /locus_tag="ACHKIR_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HFO9989400.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRINGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(16399..16647)
                     /gene="hybG"
                     /locus_tag="ACHKIR_001650"
     CDS             complement(16399..16647)
                     /gene="hybG"
                     /locus_tag="ACHKIR_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HFO9989401.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(16660..17001)
                     /gene="hypA"
                     /locus_tag="ACHKIR_001651"
     CDS             complement(16660..17001)
                     /gene="hypA"
                     /locus_tag="ACHKIR_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFO9989402.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPICHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(16994..17482)
                     /gene="hybE"
                     /locus_tag="ACHKIR_001652"
     CDS             complement(16994..17482)
                     /gene="hybE"
                     /locus_tag="ACHKIR_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HFO9989403.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARCSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMLLSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(17475..17969)
                     /gene="hybD"
                     /locus_tag="ACHKIR_001653"
     CDS             complement(17475..17969)
                     /gene="hybD"
                     /locus_tag="ACHKIR_001653"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HFO9989404.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNAPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(17969..19672)
                     /gene="hybC"
                     /locus_tag="ACHKIR_001654"
     CDS             complement(17969..19672)
                     /gene="hybC"
                     /locus_tag="ACHKIR_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HFO9989405.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(19669..20847)
                     /gene="hybB"
                     /locus_tag="ACHKIR_001655"
     CDS             complement(19669..20847)
                     /gene="hybB"
                     /locus_tag="ACHKIR_001655"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HFO9989406.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSAMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICTYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(20837..21823)
                     /gene="hybA"
                     /locus_tag="ACHKIR_001656"
     CDS             complement(20837..21823)
                     /gene="hybA"
                     /locus_tag="ACHKIR_001656"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HFO9989407.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGSGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(21826..22944)
                     /gene="hybO"
                     /locus_tag="ACHKIR_001657"
     CDS             complement(21826..22944)
                     /gene="hybO"
                     /locus_tag="ACHKIR_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HFO9989408.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(23133..23420)
                     /gene="yghW"
                     /locus_tag="ACHKIR_001658"
     CDS             complement(23133..23420)
                     /gene="yghW"
                     /locus_tag="ACHKIR_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 family protein YghW"
                     /protein_id="HFO9989409.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKYCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(23539..24426)
                     /gene="yghX"
                     /locus_tag="ACHKIR_001659"
     CDS             complement(23539..24426)
                     /gene="yghX"
                     /locus_tag="ACHKIR_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YghX family hydrolase"
                     /protein_id="HFO9989410.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVTRRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQSYPQATGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHYAELDTRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            24583..25623
                     /gene="gpr"
                     /locus_tag="ACHKIR_001660"
     CDS             24583..25623
                     /gene="gpr"
                     /locus_tag="ACHKIR_001660"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HFO9989411.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNAIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLHEMAQQ
                     RGQSMAQMALSWLLKDERVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(25663..26157)
                     /gene="yqhA"
                     /locus_tag="ACHKIR_001661"
     CDS             complement(25663..26157)
                     /gene="yqhA"
                     /locus_tag="ACHKIR_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HFO9989412.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            26348..27232
                     /gene="yghA"
                     /locus_tag="ACHKIR_001662"
     CDS             26348..27232
                     /gene="yghA"
                     /locus_tag="ACHKIR_001662"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFO9989413.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAITDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     ASYVTAEVHGVCGGEHLG"
     gene            complement(27271..27696)
                     /gene="exbD"
                     /locus_tag="ACHKIR_001663"
     CDS             complement(27271..27696)
                     /gene="exbD"
                     /locus_tag="ACHKIR_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HFO9989414.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTV
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(27703..28437)
                     /gene="exbB"
                     /locus_tag="ACHKIR_001664"
     CDS             complement(27703..28437)
                     /gene="exbB"
                     /locus_tag="ACHKIR_001664"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFO9989415.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            28689..29876
                     /gene="metC"
                     /locus_tag="ACHKIR_001665"
     CDS             28689..29876
                     /gene="metC"
                     /locus_tag="ACHKIR_001665"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HFO9989416.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFDSVEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCVLFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLLPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            30016..30675
                     /gene="yghB"
                     /locus_tag="ACHKIR_001666"
     CDS             30016..30675
                     /gene="yghB"
                     /locus_tag="ACHKIR_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YghB"
                     /protein_id="HFO9989417.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(30715..31614)
                     /gene="yqhC"
                     /locus_tag="ACHKIR_001667"
     CDS             complement(30715..31614)
                     /gene="yqhC"
                     /locus_tag="ACHKIR_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HFO9989418.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            31808..32971
                     /gene="yqhD"
                     /locus_tag="ACHKIR_001668"
     CDS             31808..32971
                     /gene="yqhD"
                     /locus_tag="ACHKIR_001668"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:1990362 - butanol dehydrogenase (NAD+)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFO9989419.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRDTKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            33076..33903
                     /gene="dkgA"
                     /locus_tag="ACHKIR_001669"
     CDS             33076..33903
                     /gene="dkgA"
                     /locus_tag="ACHKIR_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050580 - 2,5-didehydrogluconate reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HFO9989420.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIVENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            34103..35029
                     /gene="yqhG"
                     /locus_tag="ACHKIR_001670"
     CDS             34103..35029
                     /gene="yqhG"
                     /locus_tag="ACHKIR_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HFO9989421.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQPD
                     ADHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     AIASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTTNNPDEDM
                     RIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            35080..35337
                     /gene="yqhH"
                     /locus_tag="ACHKIR_001671"
     CDS             35080..35337
                     /gene="yqhH"
                     /locus_tag="ACHKIR_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HFO9989422.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSDANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(35379..37598)
                     /gene="ygiQ"
                     /locus_tag="ACHKIR_001672"
     CDS             complement(35379..37598)
                     /gene="ygiQ"
                     /locus_tag="ACHKIR_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HFO9989423.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKNPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRLQNAGAKKRPKAAVGR"
     gene            complement(37709..39121)
                     /gene="ftsP"
                     /locus_tag="ACHKIR_001673"
     CDS             complement(37709..39121)
                     /gene="ftsP"
                     /locus_tag="ACHKIR_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HFO9989424.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPTLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(39196..39933)
                     /gene="plsC"
                     /locus_tag="ACHKIR_001674"
                     /gene_synonym="parF"
     CDS             complement(39196..39933)
                     /gene="plsC"
                     /locus_tag="ACHKIR_001674"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HFO9989425.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(40167..42425)
                     /gene="parC"
                     /locus_tag="ACHKIR_001675"
     CDS             complement(40167..42425)
                     /gene="parC"
                     /locus_tag="ACHKIR_001675"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFO9989426.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDERRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVAGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(42563..44170)
                     /gene="ygiS"
                     /locus_tag="ACHKIR_001676"
     CDS             complement(42563..44170)
                     /gene="ygiS"
                     /locus_tag="ACHKIR_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO9989427.1"
                     /translation="MYTRNLLWLVSLVSAAPLYAADVPANTPLAPQQVFRYNNHSDPG
                     TLDPQKVEENTAAQIVLDLFEGLVWMDGEGQVQPAQAERWEILDGGKRYIFHLRSGLQ
                     WSDGQPLTAEDFVLGWQRAVDPKTASPFAGYLAQAHINNAAAIVAGKADVTSLGIKAT
                     DDRTLEVTLEQPVPWFTTMLAWPTLFPVPHHVIAKHGDSWSKPENMVYNGAFVLDKWV
                     VNEKITARKNPKYRDAQHTVLQQVEYLALDNSVTGYNRYRAGEVDLTWVPAQQIPAIE
                     KSLPGELRIIPRLNSEYYNFNLEKPPFNDVRVRRALYLTVDRQLIAQKVLGLRTPATT
                     LTPPEVKGFSATTFDELQKPMSERVAMAKALLKQAGYDASHPLRFELFYNKYDLHEKT
                     AIALSSEWKKWLGAQVTLRTMEWKTYLDARRAGDFMLSRQSWDATYNDASSFLNTLKS
                     DSEENVGHWKNAQYDALLNQATQITDATKRNALYQQAEVIINQQAPLIPIYYQPLIKL
                     LKPYVGGFPLHNPQDYVYSKELYIKAH"
     gene            complement(44279..44761)
                     /gene="ygiV"
                     /locus_tag="ACHKIR_001677"
     CDS             complement(44279..44761)
                     /gene="ygiV"
                     /locus_tag="ACHKIR_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFO9989428.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGMTERRLETDIYVPLA
                     "
     gene            complement(44814..45206)
                     /gene="ygiW"
                     /locus_tag="ACHKIR_001678"
     CDS             complement(44814..45206)
                     /gene="ygiW"
                     /locus_tag="ACHKIR_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HFO9989429.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            45358..46017
                     /gene="qseB"
                     /locus_tag="ACHKIR_001679"
     CDS             45358..46017
                     /gene="qseB"
                     /locus_tag="ACHKIR_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing response regulator transcription
                     factor QseB"
                     /protein_id="HFO9989430.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGDPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            46014..47363
                     /gene="qseC"
                     /locus_tag="ACHKIR_001680"
     CDS             46014..47363
                     /gene="qseC"
                     /locus_tag="ACHKIR_001680"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing histidine kinase QseC"
                     /protein_id="HFO9989431.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHTMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQANID
                     VRLTLNANGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVVDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHDMNVEFGNAEQGGFEAKVSW"
     gene            complement(47409..47741)
                     /gene="ygiZ"
                     /locus_tag="ACHKIR_001681"
     CDS             complement(47409..47741)
                     /gene="ygiZ"
                     /locus_tag="ACHKIR_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HFO9989432.1"
                     /translation="MLKKQLLSIFEALLYIMLTYWLIDAFFAFNKYDWMLESGDDICS
                     IPSVSGEDRILQAMIAAFFLLTPLIIIILRKLFVREMFEFWLYVISLVICLVCGWWLF
                     WGRFIFCY"
     gene            complement(47748..>48274)
                     /locus_tag="ACHKIR_001682"
     CDS             complement(47748..>48274)
                     /locus_tag="ACHKIR_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000834027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9989433.1"
                     /translation="KHAYAAGIRPELLGGVAWIESGGMPENYKFQIYETKRMIGSLDM
                     PENKTSFGSMGIQIRTAAITLGLDPSELTTRNQLELATCLMEDDFTFKIAATHLRDLA
                     LFDYPSSATLYMTNEQYIMAGIRYNRGVERDLGFFIYLINNLPARDTDDYKFISYGMR
                     LLEIREHIKKLINE"
BASE COUNT        12001 a        12758 c        12048 g        11467 t
ORIGIN      
        1 gcattattgt cattttattt tggatatcca attttttcgt ggtgtatatg tctaataaaa
       61 tctttacgca ttccctacct atgcgctatg ccgattttcc aacgctggtt gatgctttgg
      121 actacgccgc tctgagtagc gccggaatga atttttatga cagacgttgc caacttgaag
      181 atcaactgga atatcagacg ttaaaagcgc gttccgaagc tggtgcgaaa aggttgttat
      241 cgctgaacct gaaaaaaggc gatcgcgtgg cgctgattgc cgaaacgagt agcgggtttg
      301 tagaggcttt ttttgcctgc cagtatgccg gcttagtcgc cgtcccgttg gcgattccaa
      361 tgggcgtcgg tcagcgggat tcctggagcg ccaaactgca gggtttactg gcaagttgcc
      421 agcccgcagc cattatcact ggtgatgagt ggttgccact ggtcaatgcc gcgacgcata
      481 acaacaaccc cgaattacat gttttaagcc acgcctggtt taaggcatta ccggaagccg
      541 atgttgcgct ccagcgtcca gttccaaacg atatcgccta cctccagtac acctccggca
      601 gcacccgttt tccccgtggc gtcattatca cccatcacga agtgatggct aatctacgtg
      661 ctataagcca cgatggcatt aaattacgcc ctggcgaccg ctgcgtctcc tggctgcctt
      721 tctaccatga tatgggactg gtcggctttc tcctgacccc cgtcgccacg cagctttcag
      781 tagattattt gcgcactcag gattttgcca tgcgtcctct gcaatggctt aaattgatca
      841 gtaaaaatcg cggcaccgtt tccgttgcac cgccgtttgg ctatgaattg tgccagcgcc
      901 gcgtgaatga aaaagatctc gctgaactgg atctttcctg ctggcgcgtc gctggtattg
      961 gagcagaacc gatctccgca gaacaactcc atcaattcgc tgaatgtttt cgtcaggtta
     1021 actttgacga taaaactttc atgccgtgct acggactggc agaaaatgcg ctggctgtca
     1081 gcttctctga tgaagcctgc ggggttgtgg ttaacgaagt ggatcgcgac atcctcgaat
     1141 accagggtaa agccgtcgcg ccgggtgctg aaacacgcgc cgtatcgact ttcgtcaact
     1201 gcggtaaagc gttgccggaa catggcattg aaatccgcaa tgaagcagat atacctgtcg
     1261 cggaacgtgt ggtaggccat atttgcatct ccggccccag cctgatgagc gggtactttg
     1321 gcgaccaggt ttcgcaagac gagattgccg cgacgggctg gttagacacc ggcgaccttg
     1381 gttatctgct ggacggttat ctgtatgtca ccggacgcat taaagatctg attattattc
     1441 gtggccgtaa tatctggccg caggatattg aatacattgc ggaacaggaa ccggaaattc
     1501 attctggcga tgcgattgct tttgttaccg cccaggaaaa aatcattttg cagatccagt
     1561 gtcggatcag cgacgaagaa cgtcgcgggc agcttatcca cgcgctggca gctcggatcc
     1621 aaagcgaatt tggcgttacc gcggatatcg atctgttgcc gccccacagt attccccgaa
     1681 catcctccgg caagcctgcc cgtgcggaag cgaaaaaacg ttatcagaag gcttatgctg
     1741 ccagtcttca tgtgcaggaa tccctggcat gaatcaaact gtcgcggtga cgggcgcaac
     1801 cgggtttatc ggtaaatata ttattgataa cctgctcgcc cgcggctttc acgttcgcgc
     1861 attaacgcgt actgcccgcg ctcacgtcaa tgataatctc atctgggtgc gcggttcgct
     1921 ggaagataca cattcgctta gcgaactggt tgccggagcc agcgcggtgg tccattgcgc
     1981 cggacaagtg cgcggacaca aagaagagat ttttacccgc tgtaacgttg acggcagcct
     2041 gcgcctgatg caagcggcaa aagagagcgg cttttgccaa cgttttctgt ttatctcttc
     2101 gctggcggcg cgccatcccg aactctcctg gtacgcaaat tccaaacacg tcgccgaaca
     2161 acgactgatc gcaatggctg acgaaattac gctgggcatt tttcgcccga cagccgtgta
     2221 tggtcccggc gataaagagt taaaaccgct gtttgactgg atgctgcgcg gcctgctgcc
     2281 acgacttggt gcgccagata cacaactctc tttcctgcac gtcaccgatt ttgcgcaagc
     2341 agtgggtcag tggttaaacg ccgaaactgt acagacgcaa acctatgaat tatgcgatgg
     2401 cgtcgctggc ggctatgact ggcaacgcgt acgacagctt gccgccgaag cccgttgtgg
     2461 ttctgtgcgg atggttggca ttcctctgcc gctactcacc tgccttgcgg atatcagtac
     2521 tgcgttgagt cgcctggcag gtaaagaacc tatgctgacc cgctcgaaaa ttcgtgaatt
     2581 aacccacgcc gactggtcgg caaataataa ccgtatttct gaagatatta attggtttcc
     2641 cgggattagc ctggaacacg cattacgcaa cgggctattt tgattgtctt gtttaaaggt
     2701 gtgttaacgt ttctatggta aatcgtgaaa tagtaatgga ttacatcctt tcctgtttac
     2761 aggatttggt cgaaaacggt gtggaaatta agcccgacag cgatctcgtc aacgatcttg
     2821 gcctggagtc gatcaaagtg atggatctct taatgatgct ggaagacaga ttcgatattt
     2881 ccattcctat taatattctg ctggatgtca aaacccctgc gcaattaatg gaaactctac
     2941 ttccatggct ggagaataaa taatggggct atacgataaa tatgcgcgcc ttgctggcga
     3001 gcgcctgcaa tttagtgaca acgggttaac accatttggc acctgtattg atgaagtgta
     3061 ttccgccacc gaaggccgga tcggcaataa aaaagtgatt ctggccggga ccaataatta
     3121 tctcggttta acctttaatc atgatgctat ccgcgaaggt caggcagcgt tagcggccca
     3181 aggcactggc accaccggtt ctcgtatggc aaacggcagt tatgcgccgc atctggcgtt
     3241 agagaaagag attgccgagt ttttcaatcg ccctaccgct atcgtttttt caaccagtta
     3301 taccgctaat ttaggtgtca tcagcgcact tgccgaccat aacgccgtgg tgttactgga
     3361 tgccgacagc cacgccagta tttatgacgc ctgttctctc ggcggcgcag agattattcg
     3421 tttccgccat aatgacgtca acgatctgga acggcgcatg gtccgcctcg gtgaacgggc
     3481 gaaagaggcg attattatcg ttgaaggcat ctacagtatg ctcggtgacg ttgcgccgct
     3541 ggcagaaatt gttgatatca aacgtcgtct cggtggttat ttgattgtcg atgaagcaca
     3601 ttcgtttggc gtgttaggcg caacaggccg cggactggtt gaagccgttg gcgttgaaga
     3661 cgacgtggat attatcgtcg gcaccttcag taaaagtttg gcttccattg gtggttttgc
     3721 cgtgggttca gaagcgatgg aagtgctgcg ctacggcagc cgcccgtata tttttaccgc
     3781 ctctccttct ccgtcctgca ttgcgaccgt gcgctcgtcg ttaagaacca tttccacaca
     3841 accagagctg cggcaaaaac tgatggataa cgccaacaat ctgtatgacg gtctgcaaaa
     3901 actgggttat gagttaagtt cacatattag cccggtagtg ccagtaataa tcggttcgaa
     3961 agaggaaggt ttacgcatct ggcgcgagct tatttcgctg ggcgtttatg tgaatcttat
     4021 cctgccacct gccgcgcctg caggcatcac gctgctgcgc tgtagcgtaa acgctgccca
     4081 cagtcgtgag caaatcgatg ccatcattca ggcatttgct actctgaagc agtaatgtga
     4141 gcccgctgtg gtgttgcgcc acggcgggtt taatgttgtc tgtcatacca atacaccagg
     4201 ctcaacgcca ccagcacaaa cgctaccggt aaaccgagcg cggtaacagg tgcgctcaac
     4261 gcaaataaca atcccagatt gaccatgatt tgatagctga tccaggtgag taagccaacg
     4321 atgacgccta cagcgagacg gctacccatc ccaggcgaac gcggggcgct aaaggtaaac
     4381 ggcacagcca gcaaaatcat cgccagggtc aagatgggtt gtcccagttt ttcccacagt
     4441 gcaaggcggt attcgctgct gggttgcccg gtttcctgca aataatggat gtaatgatta
     4501 agttgacgga cagaaaaagt atttcccggc atcgataact cttcaagatt catactggtg
     4561 aaggccgatt gccagggaag attatcagat gtttcctgcg tttctttgcc attcagccat
     4621 tttttatgat tcacaccatg caggatccac gttttgtcgt cttcaatggt agcactgcgc
     4681 gcataaatgt aggattcgag cgagagatca tcgcggtaat gaaatatctc cacgtccacc
     4741 ggctggccct gctcgttcag cgatttcacc gtcacaaatt cattaccacg cctggcccac
     4801 agcatattgc cggtaatgtc atcgtcctcc cccaacgcag tggcagtcga tttgatttgc
     4861 agcgcctgtt gctgcaatgg cgacgccacc cactcatcta tcgcgcctaa tgaaacagtc
     4921 cacaatatcc cggcaaccag cgccaccagt gcaatacgga agatagaaaa ccccgtgctg
     4981 cgaatggcgg taagctcact gtttttcgat aactgcccca ggccgacaat ccctcccaat
     5041 aaggcgataa acggcgaaag ttcgaccagt gtgcgcggta aggtcatcag caccaccagc
     5101 accgcctgag tccagcgata accacccggg ctgacatcat ccagttcgtt aatcaggttg
     5161 aaggtggtaa aaagcggcag caatagacct gcggcggcgg caaaaccgag aaagagatga
     5221 cggattaaat agcgactgaa aacattcatc gggaaaattt ctgcagcaaa gagaagtcgc
     5281 gtgcgaccaa catcagcagg cccattagca tgagtccggg tactaaccac aaaccaggaa
     5341 tgaggggaat cgcgccgtta gccaccagcg tacggcagac gtcgccgcca taaaaaatgg
     5401 caacaaacag caacgtcagt ggcaataacg ttgaaaatcg cccttgccgc ggttttaccc
     5461 gacttaatga aatggccagc agcgccatca acaatgctgt caggccacgg ctttgtcgcc
     5521 attgcagttc ggcatgatcg gcaggaaagg cggagcgcgc cagctccgtg accgattttg
     5581 ctttgcgttt gacgttaggg ctttgatcca gcggattcag atgtaattgc agattacggt
     5641 agatctgttc gttgtcgtca cgcccctgat gatcgagaag ataggcggtc ccgttatgca
     5701 acataacggt cggtttctca ggtgatgggt caaccacatc aaccgaacgg gcgcggaaaa
     5761 tgcgggttcg attagcagta gaagtataaa tcagcgcatc agtcaggcga ttattatcct
     5821 gatcaaccgt ctgcgaaagg atcattcgtc cgttatcgtt agtgttaaat ttctttgccc
     5881 gcaactggcg aacatccagc tccgactgtg actgttgctc cagttgataa atctgcgcat
     5941 aagcccacgg acgaccatac atcgacagaa gggtgacaaa aatacttagc ggtatcgcca
     6001 gataaagaac ggctttgtac aagcgtcccg gactgccccc cgccgcagag atagcggtaa
     6061 tttccgagtc ggtatacatc tgccctagcg tcacgccaac tgacacatac agtccaacag
     6121 gtaacaacat ctccagtgca atcagcactt tgtagaaaac gatatccagc acaacatcaa
     6181 gagctaatgt cccatttgcc gcttcggtca gataacgctg tgcggagtag ctggcgaaaa
     6241 taaagatcag aaaaccgacg ataatcagca ccagacggcg cgtaccacgc atgatgtagt
     6301 gttcaaccag cttcatctca ctaacgccct caatcctttg aatttttatt cattgatcag
     6361 cttctgcgcc agcgcaatga catgatctac ctcttccggc gtcaggctgc cgtcctttgc
     6421 ccactgaatt tgcccggttt tattcagcac cagaatggtt gaactctgct cctgcaagtg
     6481 ccaggcaacg cgtccctgtc cattgccgtc aataacgaat tgcgcccatg gatagcggcg
     6541 tttatttttt tcgattttgc caacgacaaa atagccggta ccaaagatgg catcatcagt
     6601 gttcacaatg gtggtgggct gaaaacgatc ctgcggaaag tttgccgctt caactgcttt
     6661 aattaacagt gagttctttt tcttcgccga cttacgcccg gcgatatact gaataatgcg
     6721 aacttttccc gccaattggg tgctgttcca cttttggtag tgaatttcgt cgtcgctatt
     6781 ccgaagcagc tcgccgcctt cctggatata aacgggggta accgttttgc cagtgacgaa
     6841 attgtgtgcc tgcacacccg gggctaagag catgataatc agcgcaataa tcaaacgaga
     6901 tgacatcatt gctccggtta taccggaaaa cgcgaaatca gcagttctaa gcgctggcga
     6961 atatcgctat ccagcgccat cggtgcatca tacgactggc ggttttccat atcatccgcc
     7021 accatgtcgt cgtgtttatc ccccatcagg ccattaatgg cggcgaggaa gcgggaaatc
     7081 tgtaacgctt ctttattttc aggatcaagc gatcttaggg tgtcgtaata agaaacctgc
     7141 tggctgatga tttttcgcac atcatgaaat tccctgccgg ttagtcgcgg tttggcaatt
     7201 atctctttca gggtatgcat ttgcttttgc cgataggcaa taaaaccgct tacgctttct
     7261 aactggctat ggcgtaatga atagttcacc aacgaccaga ccggagagct taaataaacc
     7321 cgtaacaggt tgccgtaata cgacacaata tttttcttac cgttacgaaa gccgtcctga
     7381 aaacgcccca aaaaaacggt cgttttgccg aataaaaatc cggcctgatg cttctttaaa
     7441 tacagccgtt gcgcaaaacg caggtgcttg tagcgcgctc gcatgtattt aaattgtttg
     7501 cgctcatcag cagtcagctc atcgcctttg gcctgtttgc atattaaacg cagtaactcc
     7561 tggcgattga cgccattgag ccagaattgc aggcataacg cgtaattatc atggatgatt
     7621 tcggtggtac aacttagtgc gatagtcggc ggcaaactga cctgtgcatc aacgacatca
     7681 tcttcttcaa tcgcaacaaa tagacgctcg ctcgcctcta gcgggaaata ttttctcatc
     7741 atcaggattt ctctccggct gcgttataaa gcaaggaatg gcgctgaaaa aatgccttta
     7801 aggtcggaat tagcgaaagc accacatcca gacattgacc accgaaataa gcaaacgctg
     7861 ctcccagcac gccaaacaga tgacagaaca tgaacagcaa tacgatgtag ccgattgagg
     7921 cgatggtttg cgccacgaga aaagcgcgct gttttcccgc catcaatagc aatgactcct
     7981 gagggaagcc cagcatcgag atcacaattg cgcccagcat cacctgaatc aggtcatacg
     8041 cttcgagata cttaacgcca aacaccagcg aaatgagcgg cttgccgaca atcaacaccg
     8101 cgagcgccac cagaataccg attccgcccg ccagtaaacc tgacttcacg cctaacaacc
     8161 acggtctggt ggtacgcgga tctaaacgca tcacctctgg gtaaaaactt ttgcccagta
     8221 aacccgctgg cgttccggcg gcgtcgaaga atgtcatggc gattttaaat aacccggcgg
     8281 cagcgggtcc taacacgatc cccaccaaca ctgtgctgca cgaattacgc gccgaccaga
     8341 tggaatgggc aatgttggtt gaccagacaa aactccacgc gcctttgatg tgtcgggcag
     8401 actcaaacag attcaatttg aacgcgttat ggatatttct gcggcgtaat tcacgtgcgg
     8461 caaaccacca gtacatggtg ccgccaacaa gattcgaggc gtaccaggca ataacaaaac
     8521 ccgcaaaacc aaagtcaaaa taccaggcaa cgacgctccc cgctgcgcgc agaaaaggtt
     8581 tcgtcgcttg ctgtacagca attagatcga agcgatctac cgcacgcaga atgccggtcg
     8641 gcgtggagga agccattgaa ggaatgagcg tgcaatacag cgctgccagc caaaagcttt
     8701 ggtcatccag acctaatgaa tgggaaagaa aagggagtaa ggcaatgccg ccgacaatcg
     8761 ccaccgcgcc gctgacgata tccagcgaga aggaaaatga gacgacattg cggaattgct
     8821 gcggattatt gttggttaat gctggtgttc cgtactgaac caccagctgc catgtctgaa
     8881 acttaataaa atcgctgatc gacttggcgt acgattgtac aatcaccagt acgccaaaca
     8941 tggcgggcgt catcccttta ccggcacacg agagcgccaa cagacccagc aaagcgctca
     9001 cgacattact ggagcctaac caggcgctat tgcgaataat ggtgcgaaac gcgccatctg
     9061 caaaccaatg tttgatgtta aaacccgcca attcagcctg accttatcgt tgatagtaaa
     9121 aagtatcccg ccagccttaa gttaaacttc ggcggtcaga aacgatggca accagagaaa
     9181 ccgccttctg tgcctgttcc agcacttcgc tgtagggcgc tctggaatca atctcaagaa
     9241 tttttgtgcc gttatagcca atcgtcgaca tgacgccgat tttgtcctgc agctcggcat
     9301 agtcatggtc aggcttgcgg gagatggcag tatcaatatc aatccccaaa cgaatgatta
     9361 attcaggacg gtattgcgcc atttgttggt ataaacgccg ttcgcgctgc gccagaaaca
     9421 tgctgatttt cccggtcgca cgttcgacgc cgatccccgg tccgtcataa taaaagcccg
     9481 aaatttcagc ctgcgggaag cgatcgctga ccaccagaac gccactttgc gccagtcgct
     9541 gaaccttgcg tagattcgcc attcttcgca gcgagaagca gtacataatc accgccgccc
     9601 atagcgctgg agatttggtt ttcatgcttt gggttttcga ggatttggcc gccagtcgcc
     9661 gttcgagcca gacgccaacc aacggcaatc gtttgatttt gtcgccgtct tcgcccgaga
     9721 gcagccccag atagcgccgc tcggtttgcc agtgttgttg cagcgatttc accaggtcgg
     9781 tggtcagtgt ggatttaccg gtgccatcac acccgaccac cgcaatcagc cccggaatgt
     9841 agttgggctg cggcgcggtt gtactattaa cagtttgtgt ttgtagtgcg tccattcgtt
     9901 aaatcatccc ctatccattt tggtgatcag gaaagcgcgg cgtgaattgc gcgtagtgac
     9961 gcttgcagaa tttcatcggc gggttgccga ccatccagtt caaggatctt tgcgccatta
    10021 aatgtcagtt gcggcgtaac ggcgattttt tcctgtaacg ccgccagttg gtggtcaggt
    10081 ttacgcgcaa acgcggtttg ttcatcaatg ccaagacgaa tcaacaatac gggcaaataa
    10141 gacgccatcc attggtacag cttcagctcg cgctgcctta acatttttat ccaaccgtta
    10201 ccgcccgtgg tttttgccaa ttgcgggcca tcaaagcgaa aacccggcac ttcaacctgc
    10261 gggtagcggt cggtgatgag cagatagcct tgctggcttt tacacaacat tttgcgaaac
    10321 ttgtacgccc gccagcagga aagcagatag ataactagcg cagtaatatt gccaggtggt
    10381 gtggagggct tttcgtgcac atgtgccgct ttacttcgca gataacgccc aaaaggtgcg
    10441 ccaataacag ggagctgtga aatccattcg ccaattcgcc cggacgattg cccgagataa
    10501 atgtgttctg ttggcattgt tgctgccagt tcatttacca ggcttgccgt gagggtcgat
    10561 ttacctgaac catcacatcc tacaatggca ataacacgca ctggcgtgga attaattatt
    10621 gacatattac gttgattcac gaaaaacccg gcagcaaata atgtattgaa tattacgtgg
    10681 tccgtgctca gaatatccgt tcaaccttat tgtgacaatg gttaattgtg acattaacct
    10741 gaattacgct ttcataaaaa catattaatc aaataaatat tttggatgga tatttaaatt
    10801 taaggatata ttcatgcagt caataacacc tccattaatt gccgttattg gtagcgatgg
    10861 ttcaggcaag tcaacggtgt gtgaacatct tattaccgtt gtcgaaaaat atggtgctgc
    10921 cgaaagagtt catttaggaa aacaggccgg aaatgtcggt cgtgcagtga caaaattacc
    10981 gttgatggga aagtctctgc acaagactat tgaaaaaaat gaagtgaaaa cggcaaaaaa
    11041 attgccgggt ccggttccgg ccctggtgat caccgctttc gtcgcccgcc gactgttacg
    11101 ctttcgtcat atgctcgcct gtcgtcgtcg cgggctgatt gtcttaaccg atcgttatcc
    11161 tcaggaccaa attcctggcg cttacgatgg tacggtgttc ccacctaacg ttgaaggcgg
    11221 ccggtttgtc tcatggctgg caagccagga acgtaaagca tttcactgga tggcgagcca
    11281 taagcctgat ctggtcatca aactcaatgt tgaccttgaa gttgcctgcg cgcgtaaacc
    11341 cgaccataaa cgggaatcgc tggcgaggaa gattgccata acgccacagt taacctttgg
    11401 cggtgcacaa ctggttgata tcgacgccaa tcagccactg gaaaaggtgc tggttgatgc
    11461 agaaaaagcg attacggatt ttatgaccgc gcgtggttat cactagtcaa aaatggaaat
    11521 gcccgatcgc caggaccggg cattttcagg aaggttaaat caactgcaat gctatccagt
    11581 acaacccacc agaaagaaaa atcgccgccg gtaaagtaaa cacccacgcc atcaggatgc
    11641 tggttaccgt tttacgctgt aacccaccgc cgtccaccac catcgtccct gctaccgcag
    11701 acgagaggac gtgtgttgtg gagacgggca tcccaatata actggcaagg ccgatagaca
    11761 ctgccgccgt catttgtgcc gccatgcctt gcgcatacgt catgccgcgc ttaccaatct
    11821 tctcaccgat ggtcatcgct acacgacgcc agccaatcat ggtgccaatg ccgagcgcca
    11881 gagctactgc catgatgatc cacaccggag cgtactcaat ggtgcttaac atatcgctgc
    11941 gcagtttttt cagcaggttc tggtcttctt tactgacgcc tggcagtttc gccagcttcg
    12001 cggaggtatc agagatgcac agcataatgc ggcgcagctg gctgcgctga ctcacgctta
    12061 acggctcgta actttccata ttgcctggca gcatcgtttt aacgcgcgca atcgcatcaa
    12121 aggtatttgc cggatgacag tgaaactccg ttacttgcgt gccatcagtc gatgccgcag
    12181 gcaatggagg ttccatcgca atcaacttct gcggcagttc aggatgctgt tgcaggtagt
    12241 gttcgaagtt ggtgacggca tcgcgggtac gggtaatttc atagccggac gcattcatat
    12301 tgacgacgaa accagcaggg gcaatcccca ccagtaccag catcaccagg ccgatccctt
    12361 tctgcccgtc gttagcaccg tgcgaaaacg ccacgcccgc agcggaaaca atcagcgcaa
    12421 tacgcgtcca gaatggtggc ttacgtttgc cttttttctt tttgcgatct tccggaatgc
    12481 ggtgaatacg gtcacgcttt ttcgtcccgc tccagaaacg gcgcagcagg aaaatcagac
    12541 cacccgcaat gaccaggccg acgatagggg aaacaatcag cgaggagaaa attttggtca
    12601 cttcacgcag gtttaacgca tccatcaccg atgagccggt taacagcgcg ttggttaaac
    12661 cgatgccgat aatcgcacca atcaaggtgt gcgaactgga ggccggtaaa ccaaagaacc
    12721 acgttcccag gttccagata atcgcggcca gcagcatgga aaagaccatc gccaggccgt
    12781 gagttgaccc catattcagc aacaaatcgg ttggtaacat atggacaatg gcataggcaa
    12841 cgctaagtcc gcctagcaat acgccaaaaa agttaaaaaa tgccgccatc accacggcaa
    12901 gctgtggctg catggctcga gtataaataa cggttgccac cgcattcgcc gtgtcatgaa
    12961 aaccattgat tgcttcgtag aacaacacaa atgccagagc aagtaataac aaaagccctg
    13021 tgtatatatc aaggccaaca aataaattta gcatatatta gattacgcca ttttgaattt
    13081 acgaacggac gcattatcag tgactttaac ggcatgggca aagtgaaata tcttttttta
    13141 acgataaaaa gagtgttatt tcttgtgtat cagacaataa gcattcattc aaaatcttta
    13201 gaaataatca aaacacaggt gttccactgg ttaatatatt catatattaa ataaataaat
    13261 attgaaatat ataaacgttg aatgttttga ttaatccccg tactgattat tcttcataat
    13321 cagtacgggg ttgctacaac atgtattact ctttcaccac aattagcggt tcaatatcac
    13381 tctctttttt gatgaccagt gattcatcac cgcgcaaaca cgtcccaccg tagttgccgc
    13441 caacggtgaa ggtacatacc tgaatgtatt taccgtccac tttcggcaaa caccacagtt
    13501 gctgatagat gtttttctgc tcggcaaatt taccgctggt tttgtccagc acctcttcat
    13561 gatggctgac gaggtcgatg ttactgccac agcgaccggc gatcggtttc actgcataac
    13621 cggttttcac cagttcatca ttcacggaga aatcggtatc cagcaggtaa cgatggtgcg
    13681 ggaacagcga ccagaggatc ggcagaatcg ctttgttgcc ggggatcact gtccacagag
    13741 gttcaaagac cagcacttcc gggcgcagca atacgtcgat aagacgcact tcattttgcg
    13801 gatgaccggt acggatcggc accgcagcaa actcacggtc gctaacttca cgaatctgat
    13861 caaacgcggt ttcccacgcc caggttttcc acacgcagtt aaccagtcgc ccttccccat
    13921 caatcagttg cccggcagca tcccagccca gttcatccag cccgcgcaag atacgcgttt
    13981 caaagcccgc ctggtgcagc gcctgctcca taaactgcgc gtgatagttt tcctcgatat
    14041 ctttgtcctg catgatatgg acaaacggac gtgcacgact gtgtttccag gcaccagcca
    14101 gttcgttaat cagcccttcc gccggattga agccgttgcc tttatagccc tgctccgccc
    14161 aacgttcgag gatcaagcca gcttcggtat gacaggaggc ggagtcggcg ttgtattcgt
    14221 agaccttcag gccacgttcg tccatacaga aatccatacg accggtgatc atatggtgac
    14281 ggcgacgctg ccaggagaga cgcaaacgtg gccagaggat ttttgggatg tcgaacagcg
    14341 ccagcaggtt gtcatctttc agcaccttgt cggttgcgtg aagatacatc aggtgcagtt
    14401 cgttggtggc tttaatcagc tcctgctctg cgctctcggt aatggtgtag taatgatacg
    14461 gatcctgatt gatcacctga ccgttggcct gcacataggc gttttgtagc ggatcttttt
    14521 catccagcca tttaccgtca aactggcctt tgttttccag gcgcgctccg ctgattttca
    14581 acagctcgcc tgcaatttcc ggctgcggta agctgtattc agtatcttcc gtctggatca
    14641 tccagccgag aatggtggtg tcatcaaaag tgtctttaag ggtatagcag ccgttttcga
    14701 ccaccatctc cagctcgcgc gtccactgtt gcccttgcgg taacggggaa tgaatcacgt
    14761 tctgttccgc aatacggact ttgttgccat gcaattgggt aatgatggcg acatgaccag
    14821 tgtctttaaa ttcaccgcct ttatcccaga taagaagcgc acccgcgacc ggcgcacgcg
    14881 gcgagccgtt aggaaatgcc tgcaatggca ggatgttgtc attaaccact tcacgcagga
    14941 agcgcagcga gaaaatctcc cacgccatac ccacgtcagt gaagaccaca ccgtaattca
    15001 gaaagagaaa acggcgagca aattcaacgc attgccattt gtggcccata tattcgtcgt
    15061 cgatatagct acggaatacg gcgtcatctt cgtattcctg cggatcgaga gaactgtaat
    15121 ctgaagagta gattgctacc ccacctgggg cgtagcccaa taatgtcccg aacggggcat
    15181 cctggctggt cgttcctttg ctcatcactt tacctttaac aatacaacct aagcagttgg
    15241 cgtaattgtt gtgctctgat tacctgctca gcgacattaa ccggacagag gtcagactaa
    15301 tcatacacct catcgcagcg gctggcgcaa agggttaaaa aattcacatt ctgtacagca
    15361 agtgacctgc tacactgctt caacactacc actcagaagg caactcacta tgacagacaa
    15421 cacttatcag cccgcgaaag tctggacgtg ggacaaatcc gccggcggcg cgttcgccaa
    15481 tatcaatcgc ccggtttctg gtccgacgca tgaaaaaacg ctgcccgttg gcaaacaccc
    15541 attgcaactt tattcgctgg gaacgccgaa cggtcagaaa gtaacgatta tgcttgagga
    15601 gctgctggcg ctgggcgtta ctggtgcaga gtacgacgcc tggctgattc gtattggcga
    15661 tggcgatcaa ttctccagcg gctttgtcga agtgaaccca aactcgaaga tcccggcgct
    15721 gcgcgatcat acgcataatc cgccgatccg cgtgtttgag tctggatcga ttctgcttta
    15781 tctggcggag aaatttggct acttcctgcc gcaggatctg gcaaagcgta ctgaaacgat
    15841 gaactggctg ttctggctac agggggctgc accgttcctc ggcggtggtt ttggtcactt
    15901 ttaccattac gcgccggtga aaattgagta cgccatcaat cgctttacca tggaagccaa
    15961 gcgcctgctc gacgtgctgg ataagcaact ggcgcagcat aagtttgttg caggcgatga
    16021 gtacaccatt gcggatatgg cgatttggcc gtggtttggc aacgtggtgt taggcggcgt
    16081 gtatgatgcc gcagagtttc tggatgcggg aagttataaa cacgtacaac gctgggcgaa
    16141 agaagtgggc gaacgtccgg cagtgaagcg tgggcgtatt gttaaccgca tcaacggacc
    16201 gctgaacgag cagttgcatg aacgccatga cgccagtgat ttcgagacga atacggaaga
    16261 taagcgtcag gggtaagggt tggtattcgt cgcagcaagc catccaggcc ggataaggcg
    16321 ttcacgccgc atccggcaat cgagcacaat gcctgatgcg acgctgccgc gtcttatcag
    16381 gcctacaaaa gctaatcatc acgcgctggt aatgtcgtaa tccatttggc gcagcgcgtc
    16441 taatgtggct ttggcttcat cttcgtcgat gatgctcatg gcaaatccga cgtgtaccag
    16501 tacccactgg cccagcagat cggcagggtt accttcacaa atcagggcga tattcacgtc
    16561 gcgcttgata ccacatactt caacctgcgc aagctggtga atatcttcac cgacagccag
    16621 cacctggcct ggaacgccaa tacacattgt taactccggt tattcaactt caatactttt
    16681 gacgattagc gaatcgccgg tatcgacacg caaccgctcg ccatgacaga tcggacactg
    16741 cgcatcgtgc tgatgaatct ccaccacctg gctgcaatcc cagcaccagg cctgggcggg
    16801 tttatagacg atatgtaaat cgcacccttg cgccaccgtt ccgtggcaga caatttcaaa
    16861 actaaaacgg acggcgctct cctcaacgca ggagagcgca ccaatttcca gccacacggc
    16921 ggtgacgcgc ttaacatcgt gctgctccgc ctgccgttgg ataatttcaa cggcgctctg
    16981 gcaaagcgac aactcatgca ttttcgccac tcctgcggcc aaatagcagg gcacgacgcc
    17041 ctgcgtgtgg tacatccgga ttggtgactg gcagcgaaag gagcattcgg gcgcagtcat
    17101 ccgtcagccg ttggccctct tcaatcgaca tgctgtgcga aagcggcgac atcagcgagc
    17161 aggagaggta ttgcgaaaca ccatccagtt caccaacagt aaaggtcata gtgccatacg
    17221 gcagttgcag accaattttt tcactcactt tgcgcagcgg ccagagttga tccgggccgg
    17281 ggaagataac tgcactcagc atccacgggg tgatcacaca ccccgtccac tgaccttcga
    17341 acagcgtaaa gtcagaaaca tacaccggca ttgaaggatg cagaaaagag agatcatgca
    17401 tcgaacaccg ggcaatttct tcaaaagctg cctgtacttg cgccttcggg gaggtctgga
    17461 aacctgctat ctcttcagtc atgaatcgcc tcccgtggga tggcttccac gcctgattca
    17521 cgcagcgcag ccagaacctg ctcaagcgca ggttcaatca tcgcttcgac cgtcggcgtc
    17581 aagccaatgt gcggctccag tgattccggg atcacgccga ccagggtcag ctttttcgga
    17641 aactcaccgg tgaagcgcag ggccgacaat acgtcggcca ggccaagctg atgcggagag
    17701 attttgttgg taaacaacgc cggaacttct tcatctcgca ggatcatcat cgttcccggc
    17761 gcgttctttt tcgagacaat ggcatccgca ataatcagat gatcgcgatt tgccatgtcg
    17821 ccaagcagct ccattcccgc cgttccgcca tcgaggatct caacataatc cggcagaatg
    17881 tatcgttgct ctaacgcttc aacaattcgc acaccgatgg cttcatcggt cagcaaaata
    17941 ttgccaaccc ctaagactaa aatacgcatt acagaacctt cactgaaacc acttcgttgc
    18001 cgtcggcatc cactacgtgt accgcacagg ccatgcacgg gtcgaaggag tgaatggtac
    18061 gcaccacttc cagcggttta ttcggatcgg caaccggtgt acccaccagc gactgctcgt
    18121 aaggaccgac gtcatcattg aagttacgcg ggccagagtt ccaggttgat ggaacaactg
    18181 cctggtagtt actgataata ccgtctttga tcaccatcca gtgagagagc ataccgcgcg
    18241 gtgcttcaag gaaaccaacg cctttgaact cacctgtagc cggaatgttc ggcttcacaa
    18301 aggtggtgtg atcgcctttg ccgatattgg tgatcagtgc actgtattga ttttgcagga
    18361 tatcctgcaa ttcgcagcag tgaacggtac gaccaataat acggcccagc gtggagtgca
    18421 gctgcgccac ttccagcgtg ttgccagtca gtttctgata aatcgcaacg atttcattca
    18481 gtttgttctg ggtagactcg cgtcctgccg ccagtttcac cagcatgtta gccagcggac
    18541 ccacttctac cgttttgccg tagaaagtcg gtgatttcac ccaggaatat ttaccgtcgt
    18601 cagaccaacc atcataagcc ggaatggtgg taccttccca cggtgcctgc ggcgcttcgt
    18661 ctttatacca ggagtgcttc gcgctctcct gaatgccttt aatcaggtat tcatcggagt
    18721 gggaagtgat cgggcgatac gaggacagat ccgcattctc aatgtagccg cccgggaaca
    18781 ggaagctgcc gtttttgctg tcggtcggga attccggcac gctcaggtag ttcaccgcac
    18841 ctttaccgcg cgtcagccat tccgggtaga acgcggcgat aactgcggta tcaaccttgt
    18901 aaacctgctc aacaaagtcg ctcagtttat cgatgaaaga cttgatgtac atcaggcgct
    18961 caaggttcag cacgcccaga ccgtcgaggt tgatcgggtt agcgacccca cctactgcca
    19021 ggttctgaat gtgcggcgtt ttaccgccca gcagcgccac gacgcggtta gcgtcacgct
    19081 ggcactccag cgcctgcagg tagtgcgcta ccgcaatcag gttcacttcc ggcggcagtt
    19141 tcatcgccgg gtgaccccag tagccattgg cgaaaatccc caactgaccg ctggcaacca
    19201 gatctttaat cttgttctga actttggtga actcttccgg actgttcaga tgccaggtcg
    19261 aaacgccttt cagcatttca gaggctttgg ttgggtcagc ttgcagtgca gaagtgatgt
    19321 ccacccagtc cagcgccgag agctgataga aatgaacgat atggtcatgc gtggtgtgcg
    19381 cagccagaat gatgttacgg atgtattgcg cgttaaccgg aacgtcgata ttcagtgcac
    19441 tttctgccgc acgaacggaa gagagcgcgt gagtagtcgt acatacgcca cagatacgtt
    19501 gcacaatcat ccatgcatcg cgcggatcgc ggtttttcac gatctcttcc atgccgcgcc
    19561 acatggtacc ggaagcccat gctttcgaaa cgacgccatt ttcgatttcg caatcgatgc
    19621 gtaaatgccc ctcaatacgg gttaccggat caatagtaat tctctggctc atgctttgct
    19681 cgcctcatga cgattatgat cgttttgttt taaaggagga agtatcggca gtagacgaat
    19741 gagtacgatg taagtgcaaa tctcaatagc cacaaaacca atagaaatca acagttcttc
    19801 ccaggtcggg aagtaggcgt aaccgccgcc cgggttgaat gccaccagcg aataggtcag
    19861 acgccaggtt gcacaaccta acagcgcgct cagtgctgac aggaacagca tgcgggaatc
    19921 attacgcagc ttcgccacac gcagaacgac cagcgggaag agcatcagca ggacttcaat
    19981 ccagaacatc gcggagtaga agtcaccggc aaacgctaac gacagcttgt cgcgatagat
    20041 aagctcgcca aagcgcagca cgatgaaaat cgccagcaac acactgatgg tgttggtcag
    20101 cttgacgaac aggctctttt catctggacc attgccacgc agacccgcct gcaccaacga
    20161 accttcaaag atgacaatcg agaagcccat gatgaacgcc gtcagcagcg agaacagcgg
    20221 caacatttca tagctctgcc acaacggatg cactttgtag cccgccgaga tcatcagcga
    20281 ccccattgaa gactggtgca tagttggcag cagcgcaccg agcgcgataa tgaagaacat
    20341 caccttgttc agacgctgta gcgacacctt ccagcccaat cgttcaaaca atgccggagc
    20401 aaactccagt gccatcacgc cgatgtagat ggtcatacag accgccgtct cgaacagtac
    20461 cgagttcacg ttgaagtgac ccggaatgta gaagtacggc aggttccagt agcgaccaac
    20521 gtcgatggtg atcgacaagc cacccagtga gtaaccgaac agactcgcca acagcgccgg
    20581 acgcaccagc ggatggtatt gcccacggtt aaagacgtat accgcccacg ccagcgccca
    20641 gccgccacag gcaaagccgg tgccgatcaa caggtcaaac gcgatccaca cgccccacgg
    20701 gaagccgccg ttcaggtcag agacagagcc cagaccgaac accagacgct tcacaatcag
    20761 gagcatacag atgacgatta acggtccaaa aatcatgacc ggtttactga tgattttgcc
    20821 gcccagcggt tgtggatcat gactcatgat cgtctcctcc gtcgtgatgg tcgtttttgg
    20881 tgttgcgacg aaccagcacg gttaagcccg ccagcacagc cagtggtagc atcatgcctt
    20941 tatacagggt gtgttgaata ttttcggaac gcgcaccggt agaaagatcg tccagtttcg
    21001 gcaggtcgag attttcgtaa ggcacaccgg tcagtaccag aacctgagta ccgccgccct
    21061 ctttctcgcc gtacagatgc ggataatatt tcggcaccgt atgcaggtaa gtgtcgccag
    21121 atttcagcgt ctgacgcgga tagtggtatt cgctgcctgg cttcagcgcc agacgttttt
    21181 tcgcctcagc catcagctct tcacgcgtac caaaaatcac cgcgcccgcc gggcacactt
    21241 ctacgcagcc aggcagaccg cctttatcaa gacgttccac acctttctgg ttgcacagtt
    21301 cacacttatg cagcgcacca aatgggttgt tgtagtcgta cttcggcacg ttgtatgggc
    21361 aggcgaccat gcagtaacgg cagccagtgc acacatcttt gtcgtaatgg acaatgccgg
    21421 ttttcggatc ttttttcagc gcagagaccg ggcacacaga gacacaattc ggatcgacgc
    21481 agtgcataca ctgtttctta atgtacgcgt agccgttctc ctcctggtct ttgttgaccc
    21541 ccgagccgct ggtccacacc tgaatgatgt tattggtgta cggcgacagt ttgtcgttgt
    21601 tcgaccaggt ctgttcccct tgcgggttac gttcagggaa attgatatcc tgacacttgg
    21661 tgacgcaagc ctggcagcca acgcacaagg tcgagtcgta caacatcccc agcgatcccg
    21721 gaattggcgg gcggttttca gcagccgcat gactgacaga cggcagcgcg cccgtcagca
    21781 atgccccgca ggaggctgct ttaataaaat tacgtctgtt cacggttatt ctccccgtga
    21841 gtcagcgtta tctttctttt gctgacgacc cagttcacgc accgccatca cgctgacacc
    21901 ggcaaccagc ccaaccacac cgccgagcaa accaatagcg cccgcagaga cgttgccgcc
    21961 ttctttagcg ttaacgtccg gtttctgtga acgcggagtc tgattttcga cgttggcaag
    22021 ctgatggatg cctttatgga agccgatacc ttcttcgtta cagccatagc acgggtgacc
    22081 aatcgctacc ggccacacgc cgccaacgtc gcagaattgc agcgttgagc agttgccgta
    22141 tgtttctggc cctttacagc cgaggtggta caggcaccag ccttcgcggt ggccttcatc
    22201 accgaactct ttggcaaaac gaccagcatc gaagtgcggg cgacgttcgc agtgttcgtg
    22261 aatcagacgg ccataggcga aggtcggacg gtttttgtca tccagtttcg gcggcttgcc
    22321 gtaagtgatg atgtgcgcaa cggtcgcgag gaagttgtgc gggttcggcg ggcagcccgg
    22381 aatattgata acggttttgc ctggcagaac ttcttgcagg ctgactgcgc cagttgggtt
    22441 aactccagcg gcggcaacac cgccccacgc agagcaggaa ccgatagcga taatggctgc
    22501 tgcgccttcc gccgctttgc ggatgtgatc cacaatcggc tcaccggcaa ccatgcaata
    22561 aataccgtta tcttttaatg ggatggaacc atccaccact aacacatact gccctttgta
    22621 cttctcgaga gcgttatgtt tgttctcttc gacctggtga ccgaaagcag cagaaagcac
    22681 ctcgtgatac tccagagaga tagtctccag cacgaggttt tctaccgttg gatgcgttgc
    22741 acgaagcaga gattccgtac aaccggtgca ctcctgcgcg ccaatccaga taactggcgg
    22801 acgctgcggg ttagtaaccg attcggccat ctctgcagcg gctttgctac ttaaccccat
    22861 ggtggcggct aatgctgcac aaagcttcat gaaatcacga cggttaatgc cgtgagaatg
    22921 gatgagggtg ttatctccag tcatttatag ttattccgtt gcgaagacct ggcatatatt
    22981 ttgcctcaat cgcaaaatca ataatgcgat cgatgcgcca tttaccacac atttattatg
    23041 gttatcgata tcatgatact gcggcggggc gctaaaacga agggaagaag cattagtgta
    23101 attaattaaa gcaagataat acgtatgttt gatcaatttt cgaggcgata accggccata
    23161 aaaaagcgca acgtagaaca ggaattattc tcacgaaaga aatccattac catctcttta
    23221 tccagtccat agcggcgcgt cagaataccc gcttcccagg cgctaagctg gcgatctccg
    23281 gtcttttcgt acatccggtg tgagtagcct aatacaaaac cgcgtttata atcggcacag
    23341 tattttgtca cattaaccgc actgtcggca tgcgttgctt ttaatcccgc cattaagcct
    23401 ttaccaaaat ggttattcat cgttcacctc attcgctata tattgccgta tatagcgata
    23461 ttttaggcaa agaacgacat ttgcgcacgc tattttttca tgcttgttta agacgtaatc
    23521 ctgtaagaga taaacccatc aggagagata tttatcgaac catttcagcg tcctttgcca
    23581 ggcaagatcg gcggcagatt tgtcataacg gggcgtggaa tcattatgga atccgtgatt
    23641 aacccccgga tatatatacg cctcatagac cttattattg gctttcaacg ccgcctcgta
    23701 agcaggccag ccctcgttga ttcgggtgtc cagttccgcg tagtgcaaca gtaaaggcgc
    23761 ttcaatcttc gccacatcgg cagtgggtgc ctgacgacca taaaacggca ccgcgcaggc
    23821 cagttccggg taagcgaccg ctgccgcgtt cgatacgcca ccgccatagc aaaatccagt
    23881 aatacccact ttgcctgtcg cttgcggata gctttgcata aactcaatcg cggcaaagaa
    23941 atcattcatc agtttggttg gatcaacctg ctgttgcagc tcacgacctt tatcatcatt
    24001 acccggataa cctccaacgg aacttaagcc gtcaggtgcc agggcgatat accccgcctt
    24061 cgccactcgc cgcgtcacat cttcgatata cggattcagc cctcgattct catgcaccac
    24121 taccacggct ggcgttttgc cgctcatttt tgcgggcttc accagataac cccgtacctc
    24181 gccgtgacca tttggcgagg gatacgtgat gtactctgca acaatttccg ggtcggtaaa
    24241 ctctacctga gtcgccagcg cataatttgg cttgagcaaa tcaaacagcg ctaatgccgt
    24301 catcccgccc accgcatact tcgccgcaag gttgaggaac tcacgtttcg agattttccc
    24361 gtgagcgtaa tagtcgtagt aatccagcaa ctcttgtggg aaatctttgg cggttaaacg
    24421 cggcatcgtt gcactcctca actggtgttt ttttaagcaa agcataagcg cgtgtttttg
    24481 cccagttttt cgtcactctg tgagccagac tacgggatac gcgctggcga atcgctaaac
    24541 tagaaacatt gtttcgaaat tgaacgatgg aaaggagagg tcatggtctg gttagcgaat
    24601 cccgaacgtt acgggcagat gcagtaccgc tattgcggaa aaagtggttt acgcctgccc
    24661 gcgttatcgc tcggtttatg gcacaatttc ggtcacgtta acgcgctgga atcacagcgt
    24721 gcgatcctgc gtaaagcgtt tgatttaggc attacgcact ttgatttagc caacaattac
    24781 gggccgcctc caggaagcgc agaagagaac tttggtcgcc tgctgcgcga ggattttgcc
    24841 gcttatcgcg atgaactgat tatctctacc aaagccggat acgatatgtg gcccggcccg
    24901 tacggctctg gcggctcacg taaatacctg ctcgccagcc tcgaccaaag cctgaagcgt
    24961 atggggcttg agtatgtcga tatcttttac tctcatcgcg tcgatgaaaa tacgccgatg
    25021 gaagaaaccg cctctgcgct ggctcatgcg gtacaaagcg gtaaggcgct gtatgtcggg
    25081 atctcctctt actcgccaga gcggacgcaa aaaatggttg agttgctgca cgagtggaaa
    25141 attccgctgt taattcatca accttcgtac aatttactga accgctgggt ggataaaagc
    25201 ggcctgctgg ataccctgca aaataacggc gtgggctgca ttgcctttac tccactggct
    25261 cagggattgc tgaccggaaa atatctcaac gctattccgc aagattcacg gatgcatcgt
    25321 gaagggaata aagttcgtgg tctgacgccg aaaatgctca ccgaagccaa cctcaacagc
    25381 ctgcgcttat tgcatgaaat ggcacagcag cgtggacaat caatggcgca aatggcgtta
    25441 agctggttgc tgaaagatga gcgagtgacg tcggtattga ttggagccag ccgcgcggag
    25501 caacttgagg agaacgtgca ggcgctgaat aatctgacat ttagcaccga ggagctggcg
    25561 cagattgatc agcatatcgc cgatggagag ctgaatctgt ggcaggcgtc ttccgataaa
    25621 tgacctgtta ataacgggcg cgagaaccgc gcccgaatca gatcagtgat tatgacgagt
    25681 cagtcggtca agatagccca tcacaaatgc agagagcaca aacgtcagat ggataatgac
    25741 gtaccacatc agtttgttat cagggacatt tttcgcatcc ataaagacgc gcagtaagtg
    25801 aatggaagaa attgccacaa tcgacgctgc tactttgttt ttcagcgacg ttgcgtccat
    25861 tttccccagc cagttcagct tctctttgtt ctcagagata tcgagttgtg agacgaaatt
    25921 ctcgtaaccg gaaaacatca ccatcaccag caaaccgcca accagcgtca tatccaccag
    25981 cgacagcaac acgaggatca aatctgattc cgccatcgag aagatattcg gcagtacgtg
    26041 aataatctcc tggaagaact tcagcgccag ggcaactaac gccagcgaaa ggccaaagta
    26101 cacgggggca agcagccagc gagaagcata cattgcattt tcaagaaaac gttccataca
    26161 ttcctgtcgt gtagttaaaa cgggcaacag tatatcgcaa ttaagtaaac agcacgcaac
    26221 agcaaatcaa catatacgtt aaacctttgt cgcataattc tgagggttag ggatcttcct
    26281 ggttccctta tcaacgcatc gagagcgtct acccttaaag gacgttgaac ctgaagggag
    26341 aaaaatgatg tctcatttaa aagacccgac cacgcagtat tacactggtg aatatcccaa
    26401 acagaaacaa ccgacgccag gcatccaggc gaagatgaca ccggtaccgg attgcggcga
    26461 gaaaacctat gttggtagcg gtcgcctgaa agatcgtaaa gcactggtga cagggggcga
    26521 ttccggaatc ggtcgcgctg ccgccattgc ttacgcgcgt gaaggggctg acgtggcgat
    26581 cagttatctt cccgtggaag aagaagacgc tcaggatgtc aaaaagatca ttgaagaatg
    26641 cggacgcaaa gccgttctgc tgccaggcga tttaagcgat gagaaattcg cccgttcgct
    26701 ggttcacgaa gcacacaagg cgttaggcgg gctggatatt atggcgctgg tcgccgggaa
    26761 acaggttgcc attactgata ttgcagacct caccagcgaa cagtttcaaa agacctttgc
    26821 cattaacgtt ttcgcgctgt tctggctaac ccaggaagcg atccccctgc taccgaaagg
    26881 tgccagtatc atcaccactt cgtcaatcca ggcataccag ccaagcccgc atttactgga
    26941 ctatgcggct acgaaggcgg caattctgaa ctacagccgt ggcctggcaa aacaggtcgc
    27001 ggagaaaggt attcgggtga atattgtcgc gccaggcccg atctggacag cactgcaaat
    27061 ttccggcgga caaacgcagg ataagatccc gcagtttggt cagaaaacgc cgatgaagcg
    27121 tgcggggcaa ccggcggaac tggcccctgt atatgtttat ctggcaagtc aggaggcgag
    27181 ctacgtcacc gcagaagtgc atggcgtgtg cggcggcgag catttaggct aaaaaaatgc
    27241 ccggttgtga aaagcaaccg ggcatcattt ttatttcgct ttggcagttt cttcgccgac
    27301 cagacctatc ttcaggtaac ccgcctgatg cagcgtatcc attaccttca tcaacgtctc
    27361 gtaatcgacg gttttatccg ctcggaagaa gacggtggtg tctttcttgc cttcggttaa
    27421 cgcattcaac gccgtaatca ttgtttcatc ggtgaccgga tcgttaccga taaacatcga
    27481 gttgtctgcc ttcaccgaca gataaaccgg tttttccggg cgcggctgcg gcgtgctggt
    27541 agaagcaggc aagttcacct tcacatctac cgtcgctaac ggtgccgcca ccataaagat
    27601 aatcagcaga accaacatca cgtcgataaa cggcgtcacg ttgatatcat gcatttcgcc
    27661 gttatcgtcg aggttttcgt taagatgcat tgccatcgga cattatcctg cgcgtaattt
    27721 ttgtgcgaca cgaaccggat gcgcagcggc gctggcttcc agatccaggt cacggctttg
    27781 cagcaacaat acctgcgctg caacatcacc cagcatcgct ttaaagccgc caatctggcg
    27841 tgcaaatacg ttatagataa cgaccgccgg aatcgctgcc acgaggccga ttgccgttgc
    27901 taacagagct tctgcgatac ccggcgcaac gactgccagg ttagtagtct gcgtttgcgc
    27961 gataccaata aagctgttca tgatgcccca gaccgtacca aacagaccaa cgaacggcga
    28021 aatcgcgccg atggttgcca gatagccgtt accacggccc atttgacgac ctactgcggc
    28081 gacccgacgc tccagacgga agctggtacg ttctttaata ccttcgttat cgtcgctgcc
    28141 ttctgacagt tccagctcat tctgcgcttc attgagcaaa tgcaggctca agcttttgct
    28201 accaaaatca gcggcgatat cgttggcctg gtttaaggaa cgcgcttcag ccagcagttg
    28261 ctgctcgcgc ttaagacgac gcttctgatt gaagaactcc acgctcttac tgaagaagat
    28321 tgcccaggtg actacggagg ccaaaataag cccaatcatc acgcacttaa cgacaatatc
    28381 ggcgtgctga tacatacccc agacggagag gtccgtctgc attaaattat tacccactgt
    28441 gtatctccag gacgcaagtc acaaaatctg cgcataataa tatcaaaacg acgtcgaatt
    28501 gatagtcgtt ctcattacta tttgcatact gccgtacctt tgctttcttt tccttgcgtt
    28561 tacgcagtaa aaaagtcacc agcacgccat ttgcgaaaat tttctgcttt atgccaattc
    28621 ttcaggatgc gcccgcgaat attcatgcta gtttagacat ccagacgtat aaaaacagga
    28681 atcccgacat ggcggacaaa aagcttgata ctcaactggt gaatgcagga cgcagcaaaa
    28741 aatacacgct cggtgcggta aatagcgtga ttcagcgcgc gtcttcgctg gtctttgaca
    28801 gtgtggaagc caaaaaacac gcgacacgta atcgcgccaa tggcgagttg ttctatggac
    28861 ggcgtggaac gttaacccat ttctccttac aacaagcgat gtgtgaactg gaaggtggcg
    28921 caggctgtgt gctatttccc tgcggggcgg cagcggttgc taattccatt cttgcttttg
    28981 tcgaacaggg cgatcatgtg ttgatgacca acaccgccta tgaaccgagt caggatttct
    29041 gtagcaaaat cctcagcaaa ctgggcgtaa cgacatcgtg gtttgatccg ctgattggtg
    29101 ccgatatcgt taagcatctg ctgccaaaca ctaaaatcgt gtttctggaa tcgccaggtt
    29161 ccatcaccat ggaagtccac gatgttccgg cgattgttgc cgccgtacgc agtgtggtcc
    29221 cggatgccat cattatgatc gacaacacct gggcagccgg tgtgctgttt aaggcgctgg
    29281 attttggcat cgatgtttct attcaggccg ccaccaagta tctggttggg cattcagatg
    29341 cgatgattgg cactgcggtg tgcaatgccc gttgctggga gcagctgcga gagaatgcct
    29401 atctgatggg ccagatggtc gatgccgata ccgcctatat aaccagccgt ggcctgcgca
    29461 cattaggtgt gcgtttgcgc caacatcatg aaagcagtct gaaagtggct gaatggctgg
    29521 cagaacatcc ccaagtagcg cgagttaacc accctgccct gcctggcagt aaaggccacg
    29581 aattctggaa acgagacttt acaggcagca gcgggctatt ttcctttgtg cttaagaaaa
    29641 aactcaatga tgaagagctg gcgaactatc tggataactt cagtttattc agcatggcct
    29701 actcgtgggg cgggtatgaa tcgttgatcc tggcaaatca accagaacat atcgccgcta
    29761 ttcgcccaca aggcgagatc gattttagcg gaaccttgat tcgcctgcat attggtctgg
    29821 aagatgtcga cgatctgatt gccgatctgg acgccggttt tgcgcgaatt gtataacatt
    29881 gccacttttg gacaattttg cagacatttt attgtgaaaa gtcttaaatt gttgcgtccg
    29941 ggatcaaggc gtcccggacg attcaggagt acaataggca gataaaggct taaacgctgt
    30001 tccacaggaa agtccatggc tgttattcaa gatatcatcg ctgcgctctg gcaacacgac
    30061 tttgccgcgc tggcggaccc tcatattgtt agcgttgttt actttgttat gtttgccacg
    30121 ctgtttttag aaaacggctt gctgcccgcc tcatttttgc caggcgacag cttgttgata
    30181 ctggcaggcg cattgattgc ccagggggtt atggattttc tgcctacgat tgcgattctg
    30241 accgccgcag caagtctggg ctgctggcta agttatattc aggggcgctg gttagggaat
    30301 accaaaacgg tgaaaggctg gctggcacag cttcctgcta aatatcacca gcgcgccacc
    30361 tgcatgtttg accgccacgg tctgctggcg ctgctggctg gacgttttct tgcatttgtc
    30421 cgtacgctgc tgccaaccat ggcgggaatt tccggtctgc caaaccgccg cttccagttt
    30481 ttcaactggt taagcggatt gctgtgggtc agcgtggtaa ccagtttcgg ctatgcctta
    30541 agtatgattc cgttcgttaa acgccatgaa gatcaggtaa tgacgttcct gatgatcctg
    30601 ccaattgcct tgttaaccgc tggcttgttg ggcacgctgt ttgtggtgat taaaaaaaaa
    30661 tactgtaacg cctgacgatt ttccccattc ccggtagctg taccgggaac gtatttaatt
    30721 cccctgcatc gcccgcattc ttgccgcatc ttcccccggc gtcacaccga agtaacgttt
    30781 aaactcacgg ctaaattgcg atgcgctttc atagccgacg cgcatcgccg ccgcgctggc
    30841 cttcatgccg tcgtggatga tcatcatccg cgccttatgc agacggtaat tcttcaaata
    30901 ctgcaacggc gaggtgctgg tgacagactt aaaattatgg tggaacgccg atacgctcat
    30961 gttggcttct gccgccagtt gctcgacgct caggttttcg gtgtatttat tctcaatccg
    31021 tttcagcacg cgactaatca ggctgaagtg agtctggcgg ctgaccagtg ccagtaacgc
    31081 gccgccgcaa ggtccggtca gcacgtagta cagaatttcg cggatgatct gtttgccgag
    31141 aatacgcgca tccagcggtc gctccatcac atccagtaac cgctccgccg cgcataaaat
    31201 ctcttctgat aacgtggcag agttaatccc gctggctgcc atcgacggct ggaaatgctc
    31261 atcttcgcca atgtccatca acagttcctg taactgcaaa atatcgacat tgagacgcaa
    31321 ccctgccagc ggcacctctg acgtggcata ggtttcgcac tcaaacggca acggcaccgt
    31381 cagcaggaga tattcattag cgtcataacg aaacacgcgt tcattgatat aaccgatttt
    31441 atgtccggaa aagagaatta tgatgccagg ctcgtacatc accggtgtgc gtgcgaaagg
    31501 cgtctcgcca tacaacaaac gcacatcggg caacagctct gacaaactat tttctttatt
    31561 tttcagttta ttaactttat ccgccagcaa gcggcaaatc tcttcacgtt tcatatcgcg
    31621 taatttctta ggaataatgc ggcaatttga ttgtgcgcaa ttttgtagca tttctccagc
    31681 actctggaga aataggcaag acattggcag aaatgagcat tgagaggtac ggcgctggcg
    31741 accacaatga aaaacatcag gcagaccgtt ctctgccctc atattgaccc agcaaaggga
    31801 gcaagtaatg aacaacttta atctgcacac cccaacccgc attctttttg gtaaaggcgc
    31861 aatcgctggt ttacgcgaac aaattcctca cgatgctcgc gtattgatta cctacggcgg
    31921 cggcagcgtg aaaaaaaccg gcgttctcga tcaagttctg gatgccctga aaggcatgga
    31981 cgtgctggaa tttggcggta ttgagccaaa cccggcttat gaaacgctga tgaacgccgt
    32041 gaaactggtt cgcgaacaaa aagtgacttt cctgctggcg gttggcggcg gttctgtact
    32101 ggacggcacc aaatttatcg ccgcagcggc taactatccg gaaaatatcg atccgtggca
    32161 cattctgcaa acgggcggta aagagattaa aagcgccatc ccgatgggct gtgtgctgac
    32221 gctgccagca accggttcag aatccaacgc cggcgcggtg atctcccgta aaaccacagg
    32281 cgacaagcag gcgttccatt ctgcccatgt tcagccggta tttgccgtgc tcgatccggt
    32341 ttatacctac accctgccgc cgcgtcaggt ggctaacggt gtagtggacg cttttgtaca
    32401 caccgtggaa cagtatgtta ctaaaccggt tgatgccaaa attcaggacc gtttcgcaga
    32461 aggcattttg ttgacgctga tcgaagatgg tccgaaagcc ctgaaagagc cagaaaacta
    32521 cgatgtgcgc gccaacgtta tgtgggcggc gactcaggcg ctgaacggtt tgatcggcgc
    32581 tggcgtaccg caggactggg caacgcatat gctgggccac gaactgactg cgatgcacgg
    32641 tctggatcac gcgcaaacac tggctatcgt cctgcctgca ctgtggaatg aaaaacgcga
    32701 taccaagcgc gctaagctgc tgcaatatgc tgaacgcgtc tggaacatca ctgaaggttc
    32761 agacgatgag cgtattgacg ccgcgattgc cgcaacccgc aatttctttg agcaattagg
    32821 cgtgccgacc cacctctccg actacggtct ggacggcagc tccatcccgg ctttgctgaa
    32881 aaaactggaa gagcacggca tgacccaact gggcgaaaat catgacatta cgctggatgt
    32941 cagccgccgt atatacgaag ccgcccgcta agctttttac gcctcaaact ttcgttttcg
    33001 ggcatttcgt ccagacttaa gttcacaaca cctcaccgga gcctgctccg gtgagttcat
    33061 ataaaggagg aacgtatggc taatccaacc gttattaagc tacaggatgg caatgtcatg
    33121 ccccagctgg gactgggcgt ctggcaagca agtaatgagg aagtaatcac cgccattcaa
    33181 aaagcgttag aagtgggtta tcgctcgatt gataccgccg cggcctacaa gaacgaagaa
    33241 ggtgtcggca aagccctgaa aaatgcctca gtcaacagag aagaactgtt catcaccact
    33301 aagctgtgga acgacgacca caagcgcccc cgcgaagccc tgctcgacag cctgaaaaaa
    33361 ctccagcttg attatatcga cctctactta atgcactggc ccgttcccgc tatcgaccat
    33421 tatgtcgaag catggaaggg catgatcgaa ttgcaaaaag agggattaat caaaagcatt
    33481 ggcgtgtgca acttccagat ccatcacctg caacgcctga ttgatgaaac tggcgtgacg
    33541 cctgttatta atcagatcga acttcacccg ctgatgcaac aacgccagct acatgcctgg
    33601 aacgcgacac acaaaatcca gaccgaatcc tggagtccat tagcgcaagg tgggaaaggt
    33661 gttttcgatc agaaagtcat tcgcgatctg gcagataaat acggcaaaac cccggcgcag
    33721 attgttatcc gctggcatct ggatagcggc ctggtggtga tcccaaaatc ggtcacacct
    33781 tcacgcattg tcgaaaactt tgatgtctgg gatttccgtc tcgacaaaga cgaacttggc
    33841 gaaattgcaa aactcgatca gggcaagcgt ctcggccccg atcctgacca gttcggcggc
    33901 taacgtgcaa attctcccgg tggcggtaat gttccgctac cggacttttc agaaatcatt
    33961 tattcccctc gcgtcctgcc cattgttact cttccttgtt caggaatgcc aaatataagg
    34021 acatcatcat gcagagccgg aagctcttaa aggaacaact catctacacc cgggataaac
    34081 gcaacggaga ggtgaaaaac agatgaaaat aatacttctg tttttagcag ccctggcaag
    34141 ttttaccgta cacgcgcagc ctccttcaca gaccgtagaa caaacagtcc ggcagattta
    34201 tcagaactat aaatcagatg ccagtacgcc ttattttggt gaaaccggag agcgagcgat
    34261 aacttctgcg cgtattcaac aggcgcttac cctgaacgac aatcttacgc tgccgggcaa
    34321 tattggctgg ctggattatg atccggtttg tgattgtcag gattttggcg atctggtgtt
    34381 agaaagcgta gcgataaccc aacctgacgc cgatcatgcc gatgccgttg tgcgctttcg
    34441 tatctttaaa gacgataaag aaaagaccac gcagacactg aaaatggtgg cggaaaatgg
    34501 tcgttgggtc attgacgata ttgtcagcaa tcatggcagc gtcttacaag cagttaatag
    34561 cgagaatgaa aaaacgctgg ccgctatagc ttcgttgcaa aaagaacagc cggaagcctt
    34621 tgttgccgaa ctctttgaac atattgctga ttatagctgg ccgtggacgt gggtggtttc
    34681 cgactcttac cgccaggcgg tcaatgcctt ctataaaacc accttcaaga ctaccaacaa
    34741 tcccgatgaa gatatgcgaa tagaacggca atttatttac gacaatccga tctgttttgg
    34801 cgaagagtcg ctattttcac gcgttgatga aattcgagtc ctggagaaaa ccgccgattc
    34861 cgcccgcatt catgttcgtt ttacgctgac caatggcaac aatgaagagc aagaactggt
    34921 tttacagcgg cgcgaaggta agtgggaaat cgctgatttt atccgcccga atagcggtag
    34981 cctacttaag cagattgagg caaaaaccgc cgccagatta aagcaatgag ctgagttaaa
    35041 taacaattag ccggaacaat aaataaaagg gaacactata tgaaaacgat tttcaccgtg
    35101 ggagctgttg ttctggcaac ctgcttgctc agtggctgcg tcaatgagca aaaggtcaat
    35161 cagctggcga gcaatgtgca aacattaaat gccaaaatcg cccggcttga gcaggatatg
    35221 aaagcattac gcccacaaat ctatgctgcc aaatccgacg ctaacagagc caatacgcgt
    35281 cttgatgctc aggactattt tgattgcctg cgctgcttgc gtatgtacgc agaatgataa
    35341 aaaaattccc ggcagcattc tgttaccggg gatatttttt aacgtccaac cgccgcttta
    35401 gggcgtttct tcgcaccagc attctgcagg cgagattgcg tagacgacgc tttttttgcc
    35461 gtagcaggcg tctgacgctg ggtcgccatc ggcgtatgtt tagtcaacgc cggacgggta
    35521 ttgcggttct ggcgacgagc ctcacgcatc tcttcaatgg ttggcgcagg cactaagcaa
    35581 tcgcgacggc tgccaatcag atgctttttg cccatcgctt ccagcgcctg gcggattaac
    35641 ggccagtttg ccggatcgtg gtaacgcaac aacgctttat gcaggcgacg ctgcttgtcg
    35701 cccttcggta caaagacgtc ttcactttta taaccaatct tcgccagcgg gtttttgccg
    35761 gtgtaataca tagtggtcga gttagccagc ggcgacgggt agaagttctg tacctggtcg
    35821 aggcggaagc ggtgcttttt cagccacagc gccagattca ccatatcttc atcgcgcgta
    35881 cccgggtgcg cggagatgaa atacgggatc agatactgtt ctttacctgc ctgtttcgag
    35941 taagtatcga acagctcttt aaagcggtca tagctgccca tgcccggctt catcatcttc
    36001 gataacggcc cttcttcggt atgttccggg gcaatcttca gataaccgcc aacgtgatgg
    36061 gtcgccagct ctttgatata gcgcggatct tctacggcga tgtcataacg cacaccagag
    36121 gcaatcagga tctttttaat gcctttcaga tcgcgcgcac ggcgatagag gttaatcgtc
    36181 ggttcgtggt tagtgtccat atgcggacaa atatccggat aaacgcacga caaacgacgg
    36241 caggtttgct cagcgcgtgg cgatttgcaa cgcaacatat acatgttggc agtaggccca
    36301 ccgagatcgg aaatcacacc cgtaaaacct ggaacagtgt cgcggatcgc ttcgatctca
    36361 ttaataatcg aatcttcgga acggctctga ataatgcgcc cttcgtgctc ggtaatagaa
    36421 cagaaagagc agccgccaaa gcagccacgc ataatgttga ccgagaaacg aatcatttcg
    36481 taagccggaa ttcgggcatt accgtaagcc ggatgcggca cgcgcttgta cggcagcgca
    36541 aagacgctat ccatctcttc ggtagaaagc ggaatagcag gcgggttgat ccatacatag
    36601 cggtcgccat gtttttgcat caatgcgcgg gcgcagcctg ggttagtttc gtggtgaaga
    36661 atgcgcgaag catgggcgta cagcacttta tcgcccttca ctttctcgaa agaaggcagc
    36721 aacacgtagg ttttttccca cggtttcggg cgcggcggct gcacggttac ggctttcgct
    36781 tcctgctttt tcggcgcaac aggtttgtta tccgcgcacg gcagatcttc gccatacgga
    36841 tgcgggatcg ggtcgatttt cccaggagta tcaagacggg tggaatccac gccgctccag
    36901 cctggcaacg cctcttttac gataatcgcg gtattacgca catcgcggat ttcactaatc
    36961 ggctcgccca tcgccagacg atgcgccact tccaccagcg gacgctcacc gttaccgaac
    37021 atcagcatgt cggctttcga atccaccagc acggaacggc gcacggtatc ggaccagtaa
    37081 tcataatgcg cggtacggcg cagacttgcc tcaataccgc cgaggatcac cggtacatct
    37141 ttccacgcct ctttacaacg ctgggtataa accagtgtgg cgcgatccgg tcgcttaccc
    37201 gcgacgttat ccggcgtgta ggcatcgtca tgacgtaaac ggcgatcggc ggtatagcgg
    37261 ttgatcatcg agtccatgtt gccagcagta acaccgaaaa acagattcgg tttacccaga
    37321 cgcataaagt cgtctttgct gctccagtcc ggttgggcaa tgatcccgac acgaaagccc
    37381 tgcgcttcca gcatacgacc gcaaatcgcc atcccgaagc ttgggtgatc gacatacgcg
    37441 tcgccagtaa ccaaaatgat gtcgcagcta tcccagccaa gttgatccat ctcttcacga
    37501 gacatcggca aaaacggtgc cggtccaaaa caggcggccc agtactgcgg ccaggagaac
    37561 aggtcgcgat ccggttggat cagggagata gagctcattt tgcttccaga aatgataaaa
    37621 aaataatcaa aggccgggga ttataagccg gaacgaaaga gaaatcgaaa ggtattccat
    37681 actcgccctc ctcgggcgag tatgaagatt acggtaccgg attgaccaac agttgcccta
    37741 tcgagccacg gtccgccatt tccagcgtct gactgttgaa gtagaacggg aagtgcgccc
    37801 aggaaggctg accgaaataa accagcagct ccacttgtcc gtcaacccaa acggtatctt
    37861 tccagcctct gtcttccggg aacggcatcg cgccattcac gttacggatc tggaacatca
    37921 cgccttcaat atggaacgct tgcggctcgt ccgcgcgtac cgtccagcgt tcccacgttc
    37981 cttgctgcgc ggtgacatca atacggttga cgtcccacag ttgtccatta atacccgggt
    38041 catcacccag actgatatcg cgactgcgaa tcggcgaacc ggccatgatt tcagttggca
    38101 gcaggcgcat cggaagactg tctgtgacca gcggcagaag gccggttggg cgcagcgtta
    38161 gcaccagggt agaaaccaga atgctggatg gctcaaagaa gccgcgaata cgatcaacaa
    38221 tgctcgccgc ttcaccacag gtgatcgaca cttcatcacc gttgctcata tccaccagaa
    38281 tctcgcggcg ctcgcccggt gccagcgaaa gttgcttcac cgatacagga gcgggcagga
    38341 atccctgatc gccagaaatc acatgtaacg ggcgaccatc gctcatctgt agttgatagc
    38401 gacgagagtt cgacgcgttc aacagacgca agcgcaccca gccacgcgag acttcaacgt
    38461 acgggctttg tacaccgtta accagcagcg tatcaccaac aaagccgccg cttcccggtt
    38521 cgttatattc cggcgtacca aagttatcca gccgtttatc ctgaataatg accggaaaat
    38581 catccacacc ataatggttg gggataggca gcgatttgct gacttcatct tccaccagcc
    38641 acattccggc aaggccgtta tagacctgct gagccgtgcg gttgggagta ttggcgtgat
    38701 accacagagt agctgcgttc tggcgaatgg gcagtacggg tgcccagtca gcgtttggcg
    38761 acatcatccg tgccggaccg cccatcagcg ggccaggtac ctgtagcccg gccaccgtca
    38821 ttgagacatt ttctgtcagg cgattgctgt aaataagctt aacatcgtcg cccttccaga
    38881 cgcggatagt cggccccagg taacgaccat tgattcccca aaccgatgcg cgtgtccctg
    38941 gcgtaaatga ccagtgcgca cgttgtacag tcataaacag cggttgccca cggcgagatt
    39001 caagcagcgt cggaacgggt agcggttgct gttgcccggc tgcgctggcc ttcaggggaa
    39061 cagcgcctgc acaaagtgca atccccgatg cctgaatgaa ctgacgccga ctgagtgaca
    39121 tatttgctcc atgtaaaact ggctaaagta ataccgtgca ggaaagtgtt ccccgcaaaa
    39181 atacagattg ccgacttaaa cttttccggc ggcttcgcgc tctgcgactt ctttatcgag
    39241 ctcagcgatt ttttgttcca ttatcgaacg acaatgggca gccaactcgc gaacctgatc
    39301 tttgccatac tgactgacgt caattggcgg cagcatttcg acaatcacca gaccgttgtg
    39361 tagccgatta agattaatct tattcgaagt tgtagagacg cacacgggaa taatcgggac
    39421 gcccgccgca attgccgcgt gaaatgcccc ggtcttgaac ggcagcaggc cgcgaccacg
    39481 gctgcgggtt ccttccggga acatccagat ggaaatacgg cgttttttga agtgattcac
    39541 gacttccgca atagtgccgt gagctttagt gcgattgttt ctgtcgatca ataagttgcc
    39601 ggttaaccag tacaactgcc cgaagaaggg gatccacagc aagctctttt tccctaccgt
    39661 cactgtcggc ggctgcacaa tgttcgatgc cgtcaccatg tcataattgt tctggtggtt
    39721 agcaatatag atagcatttc cgtagctttc agcatccgca ggtttgcggc actcaacttt
    39781 caggccgaac agcggtgcaa gacggccaaa catatgccca aaggtggcca catgtttcgg
    39841 gttacgcggg ctgaaaaggc agtaaatgga gccgaataca cagactaaga tgctgtaaat
    39901 cacggtaata ataagacgaa agatatatag cataacaccc tctgaaggtc tgagagactg
    39961 gcattgtacg tcacctgaaa tcaggttagg gcattgttaa aagccgctct ctggaaaagc
    40021 gtattgttaa tcgcaacgca tgaataaaca acggtttcaa gggaattttt atcgtttccg
    40081 ggggttgaat acgctgccgg atgacgactt aacgtttcat ccggcgttcc ttgcaagcgg
    40141 gaggaaacag cgccctcccc ggcatattac tcttcgctat caccgctgct ggcacggcga
    40201 ggagagtcga tctcaacacg atcgatacgc tgcaaaccgc gcatcaacgt accgcggcgt
    40261 ccacgttcgc cagcgacttt ctgtagctct tccggacgca gtttaatttt gcgtttccca
    40321 acatgaatgg tcagcgtgct ttgcggcggc agaacgtaca gttgcgcaag accatcctcg
    40381 ccacgcgcgg cttctgccga tggaatgttg ataatcttgt tgcctttgcc cttcgacagc
    40441 tgcggcagat cgcttaccgg gaacatcaac atacggcctg cctgagtgat tgccagcagc
    40501 atatcggaag catcttcaat caccaccggc ggcataacat gggcattttc cggtaaggtg
    40561 atcaaagcct tacctgcacg gttacgcgcc accagatcgt taaaggtgca gacgaaaccg
    40621 taacccgcat cggaagccat cagcagtttc tgatcgtcgc tttccatcag catatggtca
    40681 acggtcgctc caggcggcaa cgttaatttg ccggtgagcg gctcaccctg accacgcgcc
    40741 gacggcagcg taatcgggtc gatggcatag ctacgaccgg tggaatcaac aaacactacc
    40801 ggttggttgc tcttaccttt caccgccgct ttgaagctat cgcccgcttt ataattcagg
    40861 cccggcgcgt caatatcatg gcctttagcg ctgcgcaccc agcccatctg cgacagcaca
    40921 atggtgacag gttcagacgg cagcatgtcg tgctcgctca ttgctttcgc ttcttcgcgt
    40981 tcctgcaacg gcgaacgacg ttcgtcaccg taggcttgcg cgtctgcctg cagttctttc
    41041 ttcagcaggt tattcatttt acgctcggaa gccaaaatgc cctgcaactg gtcgcgctct
    41101 ttttccagct cactctgctc accgcgaatc ttcatctctt ccagtttggc aagatgacgc
    41161 agtttcagtt cgaggatcgc ttccgcctgg gtttcagtaa ggccaaaccg cgacatcagc
    41221 gccggtttcg gttcatcttc attacgaatg atctcaatca cttcgtcgat attgagaaac
    41281 gccaccagca aaccttcgag gatatgcagg cgcttgagga ctttctccag acgatagttc
    41341 agtcggcggc gcacggtatc gcggcggaac accagccatt cggagaggat ttccagcagg
    41401 tttttcaccg ccggacgacc atccagaccg atcatattga ggttaatgcg atagctcttt
    41461 tccagatcgg tggtagcaaa gaggtggttc atcacctgat ccatatccac gcggttggaa
    41521 cgcggcacaa tcaccagacg ggtcgggttc tcgtggtcag attcatcgcg cagatcgtca
    41581 accatcggca gctttttgtt gcgcatttgc gcagcaattt gctccagtac gcgcgcacct
    41641 gaaacctgat gcggcaatgc gctgataacc accgcgccat cttctttctt ccaaaccgcg
    41701 cgcatacgca ctgaaccacg tccgttctcg tagattttac gaatctcggc gcgcgaagtg
    41761 ataatttccg cttcagtcgg ataatccggc ccctgcacga tatccagcag ctgatcgagc
    41821 gtggttttcg gctggtcgat taatgcgatt gccgcctgag ccacttcacg caggttatgc
    41881 ggtggaatat cggtcgccat gccgacggca ataccggtgg tgccgttaag caaaatgttt
    41941 ggcagacggg caggcagcat tttcggctcc tgcaaagtgc cgtcgaagtt tggcacccag
    42001 tcagccgtcc cctgccccag ctcgctcaat agcagctcgg aatatttcga caaccgggat
    42061 tcggtgtaac gcattgccgc gaacgatttc ggatcgtccg gcgcgcccca gttcccctga
    42121 ccatcaacca gcggataacg gtaagagaac ggctgcgcca tcaggaccat cgcttcataa
    42181 caggcactat cgccgtgcgg atggtattta cccagtacgt caccgacggt acgggccgat
    42241 tttttaaatt tggcgctggc attcaggccc agctcagaca tcgcatacac aatgcggcgc
    42301 tgaacaggtt tcagaccatc accaataaac ggcaacgcac ggtccatgat cacgtacatg
    42361 gagtagttta agtaggcgtt ttccgtaaat tcatgtagcg caaggcgctc tgccatatcg
    42421 ctcattaatt ctgattcctc aacttattcg ccagccttca aaggggcaat attgccggag
    42481 atactacatt atctgccgcg tcgagtcaca ggagatcagc gtgaatagcg ccgccggacg
    42541 tgctccggcg gcgataaagg catcaatgtg ccttgatata caactctttg ctgtagacat
    42601 aatcctgggg attatgcagc ggaaaaccgc caacgtaggg tttaagcagt ttgattaacg
    42661 gctgatagta gatgggaatc agcggtgcct gctggttgat gatcacttct gcctgctgat
    42721 acaacgcatt acgctttgtc gcatcagtga tctgcgtggc ctggtttagt aaggcgtcat
    42781 actgcgcatt tttccagtga ccgacgtttt cttcactatc gcttttgagc gtgttcagga
    42841 agctggaggc atcattgtac gtcgcatccc acgactgccg agacagcata aaatcaccgg
    42901 ctcgtcgggc atcaagatag gttttccact ccattgtgcg cagcgtcacc tgtgcaccca
    42961 gccatttttt ccattcggaa gacaacgcta tcgcggtctt ttcatgcaga tcgtacttgt
    43021 tgtagaacag ctcaaagcgt agcggatgag aggcgtcgta tcccgcctgt ttcagcaagg
    43081 cttttgccat cgcgacacgc tcactcattg gcttttgcag ttcatcgaac gtcgtcgcgc
    43141 taaagccttt tacctctggc ggcgtcagcg tggttgcggg cgttctcaac cccagtacct
    43201 tttgcgcaat aagctgtcga tcaaccgtaa gatatagcgc ccgacgcacc cgcacatcgt
    43261 taaatggcgg tttctcaagg ttaaagttgt aatattcgct gttcagacgc gggataattc
    43321 gtagctcgcc aggcagtgat ttttcaatgg cgggaatttg ctgcgccgga acccaggtga
    43381 gatcgacctc tcccgcgcga tagcggttat agccggtgac cgaattatcc agcgccagat
    43441 actcaacctg ttgcaatacc gtatgttgcg catcgcggta ctttggattt ttgcgtgcgg
    43501 taatcttttc gttaactacc catttatcaa gcacaaaggc accgttgtaa accatgttct
    43561 ctggcttact ccagctatcg ccatgtttag cgatgacatg atgaggaacc gggaacaacg
    43621 tcggccaggc gagcatcgtc gtaaaccaag gaactggctg ctcaagcgta acttcaagag
    43681 tacgatcatc cgtcgctttg atccccagcg atgtaacatc tgctttaccc gcaacaatag
    43741 ccgcggcatt gttaatgtgc gcctgtgcca gatagccagc aaaagggctt gccgttttcg
    43801 ggtcaaccgc acgttgccag ccgaggacaa aatcctctgc cgtcagaggc tgaccgtctg
    43861 accactgcaa accgctacgc agatggaaaa tatagcgctt gccgccgtca agtatctccc
    43921 agcgttcagc ctgagcgggc tgcacctggc cttcaccgtc catccatacc agaccttcaa
    43981 acagatccag cacaatctgc gcggcagtat tctcctctac cttttgcggg tcgagcgtgc
    44041 ctgggtcgct atgattgttg taacgaaaga cttgttgcgg ggcgagcggt gtgttggcgg
    44101 gaacgtcagc agcgtagaga ggagccgcac ttaccaggct gaccagccat aataaatttc
    44161 gcgtatacat ggtattcctt accggagaaa ttataagccc gacaacaata cgcctgtggc
    44221 attgtttgct caaacttatc gcgagtgatt tggctcacac tccggtaaag attccccatc
    44281 acgccaacgg cacataaata tccgtttcca gtcgccgctc tgtcatccct tcggcaagat
    44341 tggtgtagtg aaacaggatc ggggctttgc gcattttttc gccgcttgca ggtagccagt
    44401 ggcgaatgat gccccatatc gtgtgtgaaa tatcgtccag ctcgccaaca tggcgggcaa
    44461 cagcataacg cccaccggta agttcgccat tactcacgcc ataacgatta tcggggatcg
    44521 gttcgctaac gctgccgcag atatcaaagc gaaacgcttc tggtgcggtg gtggctgggt
    44581 cgtcccaggc gacgccaaaa gtctggcttt ggttaacagg agaaagcccc gtctctttac
    44641 gccacatgat aaatttcgcc acgctgtagt tgagcaactc agggctacag cggtgcggca
    44701 acatcgccac agggattgat gggaaatcga taatatttac atccagtgtc aggtttgtca
    44761 ttttgcctcc ctgcgaaatt attcccggga acgcgtcccg ggagcggtaa caattacgga
    44821 tttactttgc ggatctgttt gacgtcaatt tcaacagagt tccagtcttt atcgacttcc
    44881 ccctgaatct caaccgtatc tttcggcgtc accgtcacgc cgttccagcg tttatggtcg
    44941 atatcaacat tgatagtacc gctggcatct ttgaacacgt agagatcgtc agagatgcgt
    45001 tcaacgatat tgccgcgtag ggttacccag gtgtcgtcac gcagggattt tgcgctttct
    45061 acagtcgtta cgctgccgtt cggcccctgg aatcctccgg cctgactttg cgttgccgat
    45121 gggccagaaa aacctccctg ctctgctgcc ataaccggtg cactgcacag ggccattact
    45181 gcgattactg ctgcgaattt tttcatgttt attactccct ttaatgtctg tttccgagca
    45241 tttaacaaga tagtccttaa caacttctta agggaaaaaa ataaaattta atgctgtaca
    45301 gagcgcgtta caacacggtt tactggcagc aaatacggtt atcgcaggga tgaaaaaatg
    45361 cgaattttac tgatagaaga tgacatgctg attggcgacg gcatcaaaac gggccttagt
    45421 aaaatgggtt ttagcgtcga ctggtttaca caaggtcgtc agggaaaaga ggcgctatat
    45481 agcgcacctt atgatgcggt gatcctggat ttaaccttac cgggcatgga tggtcgcgat
    45541 attttgcgcg aatggcgaga aaaaggtcag cgtgagccgg tattgatcct gactgcgcgc
    45601 gatgcgctgg cggaacgtgt agaagggctg cgtctgggag ctgacgatta tctgtgtaaa
    45661 ccttttgcgt tgatagaagt cgccgccagg ctggaagctc tgatgcgccg aaccaacggc
    45721 caggccagca acgagctgcg ccacggcaac gtcatgctag accccggcaa acgtatcgcc
    45781 acgctggctg gcgacccctt aacgctgaaa ccaaaagaat ttgccctgct ggaattactg
    45841 atgcgtaacg ctggtcgggt actgccgcgc aaactgattg aagagaaact gtatacctgg
    45901 gacgaagagg tcaccagtaa tgccgttgaa gtgcatgtgc atcatctgcg acgcaaactc
    45961 ggtagtgatt ttattcgtac cgtgcatggt attggctaca cattaggtga gaaatgaaat
    46021 ttacccaacg tcttagtctg cgagtcaggc tgacgctaat ctttttaatt ctggcctcgg
    46081 tgacctggct gctttccagc tttgtcgcct ggaaacaaac aacggataac gtcgatgagt
    46141 tgttcgacac ccaactgatg ctgtttgcca aacggttaag tacgctcgat ctcaacgaaa
    46201 tcaacgcggc ggatcgcatg gcacagacgc caaataaatt aaaacacggt catgttgatg
    46261 acgatgcgct gacttttgcc atctttaccc acgacggcag aatggtcctt aacgatggcg
    46321 ataacggaga agatattcct tatagctatc agcgggaagg ttttgctgac gggcaactgg
    46381 tcggtgaaga cgatccctgg cgttttgtct ggatgacctc acctgatggc aaatatcgca
    46441 tcgttgtggg tcaggaatgg gaataccgtg aagacatggc gctggcgatt gttgccggac
    46501 aattgatccc gtggctggtc gcactgccaa ttatgttaat catcatgatg gtactactgg
    46561 gtcgtgaact cgcgccgctg aacaaactgg cgctggcact acgtatgcgt gaccctgact
    46621 cggaaaaacc actaaacgcg acgggcgtac ccagcgaagt gcgcccactg gttgagtcgc
    46681 taaatcaact gttcgcccgc acacatacga tgatggttcg tgaacgacgc tttacctccg
    46741 acgcagctca cgaacttcgt agcccgttaa cggcgctgaa agtgcaaacc gaagttgctc
    46801 agctctctga cgatgatccg caggcgcgga aaaaagcgct gcttcaatta cattccggaa
    46861 tcgatcgtgc tactcgtctg gttgatcaac tgctcacgct atcgcggctg gactcactgg
    46921 ataaccttca ggacgtcgcg gagatcccgc ttgaagatct cctgcaatcg tcggtgatgg
    46981 atatttacca cacggcgcag caggcgaaca ttgacgtgcg actgacactc aatgccaacg
    47041 gcatcaaacg caccggacaa ccgctattgc taagtttgtt ggtgcgaaat ttgctggata
    47101 acgccgtgcg ctacagtcca cagggcagcg tggtggacgt cacgctgaat gccgataatt
    47161 tcatcgtgag ggataacggc cccggtgtga caccagaggc actggcgaga attggcgaac
    47221 gcttctatcg cccacccgga caaaccgcta ccggcagtgg acttgggcta tcaattgtcc
    47281 agcgaatcgc caagctgcat gacatgaatg ttgaatttgg gaatgcggaa caaggtggat
    47341 ttgaggcgaa agtaagctgg taacttctct gtctatgaga gccgttaaaa cgactctcat
    47401 agattttatt aatagcaaaa tataaaccgt ccccaaaaaa gccaccaacc acaaaccaaa
    47461 caaataacga gcgagattac atataaccaa aactcaaaca tttctcgcac aaagagtttt
    47521 cttaagataa taataataag tggcgtcaga agaaaaaatg ctgcaatcat tgcttgcaat
    47581 atacgatctt cgcctgaaac ggaaggtata ctgcaaatat catctcccga ctcaagcatc
    47641 cagtcatact tattgaaagc aaaaaaagca tctattaacc aataagtaag cattatgtat
    47701 aatagtgctt caaatattga tagtagttgt ttttttaaca tctctgatta ctcattaatc
    47761 aattttttga tatgttctct aatctctaac aacctcatac cataagagat gaatttataa
    47821 tcatctgtat cacgcgcagg caaattatta attaaataaa taaaaaaacc caaatctcgt
    47881 tcaacaccac gattatatct aataccagcc ataatatact gctcattcgt catatacagt
    47941 gttgccgaag agggataatc aaaaagggca agatctctta aatgagtagc agcaatttta
    48001 aaggtaaaat catcttccat caggcatgtc gctaactcta actgatttct ggtagttaat
    48061 tctgaagggt caaggcctag agtaatcgct gcggttcgta tttgtatacc cattgagcca
    48121 aatgatgttt tattttcagg catatctaac gatccgatca ttctttttgt ttcatatatc
    48181 tgaaacttat aattttcagg catacctcct gactcaatcc aggccacgcc gcccagtaac
    48241 tctggacgaa tccctgcggc atatgcatgt ttaa
//
LOCUS       DAZGQL010000025        47164 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.025, whole genome shotgun sequence.
ACCESSION   DAZGQL010000025 DAZGQL010000000
VERSION     DAZGQL010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 47164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47164
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.025"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            146..226
                     /locus_tag="ACHKIR_001683"
                     /pseudo
     CDS             146..226
                     /locus_tag="ACHKIR_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310173.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(271..2316)
                     /gene="dcp"
                     /locus_tag="ACHKIR_001684"
     CDS             complement(271..2316)
                     /gene="dcp"
                     /locus_tag="ACHKIR_001684"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HFO9989434.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQAPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDFAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAELRDRATREKLFTAGWTRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELVSIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDESQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KSAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGRRFREAILSRGNSEDLERLYRQWRGKAPQIMPMLQHRGLNV"
     gene            2453..3199
                     /gene="ydfG"
                     /locus_tag="ACHKIR_001685"
     CDS             2453..3199
                     /gene="ydfG"
                     /locus_tag="ACHKIR_001685"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HFO9989435.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDSKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            3288..3974
                     /gene="rspR"
                     /locus_tag="ACHKIR_001686"
                     /gene_synonym="ydfH"
     CDS             3288..3974
                     /gene="rspR"
                     /locus_tag="ACHKIR_001686"
                     /gene_synonym="ydfH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator rspR"
                     /protein_id="HFO9989436.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLEIFSALQKRDGDAVERAMTHHLQEISESVRQIR
                     QENSDWFSEE"
     gene            4151..4354
                     /gene="ydfZ"
                     /locus_tag="ACHKIR_001687"
     CDS             4151..4354
                     /gene="ydfZ"
                     /locus_tag="ACHKIR_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HFO9989437.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(4390..5850)
                     /gene="ydfI"
                     /locus_tag="ACHKIR_001688"
     CDS             complement(4390..5850)
                     /gene="ydfI"
                     /locus_tag="ACHKIR_001688"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFO9989438.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQI
                     DGLETVLAVMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVVADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEENVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYALMLQEQAP
                     TLKVQGVDLQDYANRLIERYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(5940..7307)
                     /gene="ydfJ"
                     /locus_tag="ACHKIR_001689"
     CDS             complement(5940..7307)
                     /gene="ydfJ"
                     /locus_tag="ACHKIR_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
                     /protein_id="HFO9989439.1"
                     /translation="MTIEKHERSTKDLVKAAVSGWLGTALEFMDFQLYSLGAALVFHE
                     IFFPESSTAMALILAMGTYGAGYVARIVGAFIFGKMGDRIGRKKVLFITITMMGICTT
                     LIGVLPTYAQIGVFAPILLVTLRIIQGLGAGAEISGAGTMLAEYAPKGKRGIISSFVA
                     MGTNCGTLSATAIWAFMFFILSKEELLAWGWRIPFLASVVVMVFAIWLRMNLKESPVF
                     EKVNDSNQPTAKPAPAGSMFQSKSFWLATGLRFGQAGNSGLIQTFLAGYLVQTLLFNK
                     AIPTDALMISSILGFMTIPFLGWLSDKIGRRIPYIIMNTSAIVLAWPMLSIIVDKSYA
                     PSTIMVALIVIHNCAVLGLFALENITMAEMFGCKNRFTRMAISKEIGGLIASGFGPIL
                     AGIFCTMTESWYPIAIMIMAYSVIGLISALKMPEVKDRDLSALEDAAEDQPRVVTAAQ
                     PSRSL"
     gene            complement(7365..8384)
                     /gene="rspB"
                     /locus_tag="ACHKIR_001690"
     CDS             complement(7365..8384)
                     /gene="rspB"
                     /locus_tag="ACHKIR_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent oxidoreductase"
                     /protein_id="HFO9989440.1"
                     /translation="MKSILIEKPNQLAIIEREIPTPSAGEVRVKVKLAGICGSDSHIY
                     RGHNPFAKYPRVIGHEFFGVIDAVGEGVESARVGERVAVDPVVSCGHCYPCSIGKPNV
                     CTTLAVLGVHADGGFSEYAVVPAKNAWKIPEAVADQYAVMIEPFTIAANVTGHGQPTE
                     NDTVLVYGAGPIGLTIVQVLKGVYNVKNVIVADRIDERLEKAKESGADWAINNSQTPL
                     GESFAEKGIKPTLIIDAACHPSILKEAVTLASPAARIVLMGFSSEPSEVIQQGITGKE
                     LSIFSSRLNANKFPVVIDWLSKGLIKPEKLITHTFDFQHVADAISLFEQDQKHCCKVL
                     LTFSE"
     gene            complement(8396..9610)
                     /gene="rspA"
                     /locus_tag="ACHKIR_001691"
     CDS             complement(8396..9610)
                     /gene="rspA"
                     /locus_tag="ACHKIR_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HFO9989441.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPQLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNW"
     gene            complement(9816..10142)
                     /gene="ynfA"
                     /locus_tag="ACHKIR_001692"
     CDS             complement(9816..10142)
                     /gene="ynfA"
                     /locus_tag="ACHKIR_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HFO9989442.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLSLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            10277..10618
                     /gene="ynfB"
                     /locus_tag="ACHKIR_001693"
     CDS             10277..10618
                     /gene="ynfB"
                     /locus_tag="ACHKIR_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HFO9989443.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            10653..11213
                     /gene="speG"
                     /locus_tag="ACHKIR_001694"
     CDS             10653..11213
                     /gene="speG"
                     /locus_tag="ACHKIR_001694"
                     /EC_number="2.3.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
                     /protein_id="HFO9989444.1"
                     /translation="MPSAHSVKLRPLEREDLRYVHQLDNNASVMRYWFEEPYEAFVEL
                     SDLYDKHIHDQSERRFVVECDGEKAGLVELVEINHVHRRAEFQIIISPEYQGKGLATR
                     AAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFTVEGELMHEFFINGQYRNAI
                     RMCIFQHQYLAEHKTPGQTLLKPTAQ"
     gene            complement(11216..11926)
                     /gene="ynfC"
                     /locus_tag="ACHKIR_001695"
     CDS             complement(11216..11926)
                     /gene="ynfC"
                     /locus_tag="ACHKIR_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HFO9989445.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAQT
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            12034..12339
                     /gene="ynfD"
                     /locus_tag="ACHKIR_001696"
     CDS             12034..12339
                     /gene="ynfD"
                     /locus_tag="ACHKIR_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HFO9989446.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYTRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            12538..14964
                     /gene="ynfE"
                     /locus_tag="ACHKIR_001697"
     CDS             12538..14964
                     /gene="ynfE"
                     /locus_tag="ACHKIR_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfE"
                     /protein_id="HFO9989447.1"
                     /translation="MSKNEQMVGISRRTLVKSTAIGSLALAAGGFSLPFTLRSAAAAV
                     QQASEKVVWGACSVNCGSRCALRLHVKDNEVTWVETDNTGSDEYGNHQVRACLRGRSI
                     RRRINHPDRLNYPMKRVGKRGEGKFERISWDEALDTIASSLKKTVEQYGNEAVYIQYS
                     SGIVGGNMTRSSPSASAVKRLMNCYGGSLNQYGSYSTAQISCAMPYTYGSNDGNSTTD
                     IENSKLVVMFGNNPAETRMSGGGITYLLEKAREKSNAKMIVIDPRYTDTAAGREDEWL
                     PIRPGTDAALVAGIAWVLVNENLVDQPFLDKYCVGYDEKTLPADAPKNGHYKAYILGE
                     GDDKTAKTPQWASQITGIPVDRIIKLAREIGTAKPAYICQGWGPQRQANGELTARAIA
                     MLPILTGNVGISGGNSGARESTYTITIERLPVLDNPVKTSISCFSWTDAIDHGPQMTA
                     IRDGVRGKDKLDVPIKFIWNYAGNTLVNQHSDINKTHEILQDESKCEMIVVIENFMTS
                     SAKYADILLPDLMTVEQEDIIPNDYAGNMGYLIFLQPVTSEKFERKPIYWILSEVAKR
                     LGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPALPSYDELKKMGIYKRKDPNGHFVAYK
                     AFRDDPEANPLKTPSGKIEIYSSKLAEIARTWELEKDEVISPLPVYASTFEGWDSPER
                     STFPLQLFGFHYKSRTHSTYGNIDVLKAACRQEVWINPIDAQKRGIANGDMVRVYNHR
                     GEVRLPAKVTPRILPGVSAMGQGAWHEANMSGDKIDHGGCVNTLTTLRPSPLAKGNPQ
                     HTNLVEIEKI"
     gene            15025..17448
                     /gene="ynfF"
                     /locus_tag="ACHKIR_001698"
     CDS             15025..17448
                     /gene="ynfF"
                     /locus_tag="ACHKIR_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfF"
                     /protein_id="HFO9989448.1"
                     /translation="MKIHTTEALMKAEISRRSLMKTSALGSLALASSAFTLPFSQMVR
                     AAETPVEEKAVWSSCTVNCGSRCLLRLHVKDDTVYWVESDTTGDDVYGNHQVRACLRG
                     RSIRRRMNHPDRLKYPMKRVGKRGEGKFERISWDEALDTISDNLRRILKDYGNEAVHV
                     LYGTGVDGGNITNSNVPYRLMNSCGGFLSRYGSYSTAQISAAMSYMFGANDGNSPDDI
                     ANTKLVVMFGNNPAETRMSGGGVTYYVEQARERSNARMIVIDPRYNDTAAGREDEWLP
                     IRPGTDGALACAIAWVLITENMVDQPFLDKYCVGYDEKTLPANAPRNAHYKAYILGEG
                     PDGIAKTPEWAAKITSIPAEKIIQLAREIGSAKPAYICQGWGPQRHSNGEQTSRAIAM
                     LSVLTGNVGINGGNSGVREGSWDLGVEWFPMLENPVKTQISVFTWTDAIDHGTEMTAT
                     RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTHEVLQDDSKCEMIVGIDHFMTAS
                     AKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFERKPIYWMLSEVAKRL
                     GPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEEMKTTGIFKKKCPEEHYVAFRA
                     FREDPQANPLKTPSGKIEIYSERLATIADTWELKKDEIIHPLPAYTPGFDGWDDPLRK
                     TYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARGIRHGDTVRVFNNNG
                     EMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILTSHRPSPLAKGNPSH
                     SNLVQIEKV"
     gene            17459..18076
                     /gene="ynfG"
                     /locus_tag="ACHKIR_001699"
     CDS             17459..18076
                     /gene="ynfG"
                     /locus_tag="ACHKIR_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO reductase like molybdoenzyme iron-sulfur
                     subunit YnfG"
                     /protein_id="HFO9989449.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            18078..18932
                     /gene="ynfH"
                     /locus_tag="ACHKIR_001700"
     CDS             18078..18932
                     /gene="ynfH"
                     /locus_tag="ACHKIR_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate reductase membrane anchor subunit YnfH"
                     /protein_id="HFO9989450.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAANNDADRQRIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWYNGYTTLAFFLTVLLS
                     GPILAATILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCTGLGCWLCPLIRRREPHVAGLILGLILILGGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            18975..19589
                     /gene="dmsD"
                     /locus_tag="ACHKIR_001701"
     CDS             18975..19589
                     /gene="dmsD"
                     /locus_tag="ACHKIR_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HFO9989451.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTRDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            19748..21040
                     /gene="clcB"
                     /locus_tag="ACHKIR_001702"
     CDS             19748..21040
                     /gene="clcB"
                     /locus_tag="ACHKIR_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HFO9989452.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYSVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAKHS"
     gene            complement(20993..21688)
                     /gene="bioD"
                     /locus_tag="ACHKIR_001703"
     CDS             complement(20993..21688)
                     /gene="bioD"
                     /locus_tag="ACHKIR_001703"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFO9989453.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(21813..23033)
                     /gene="mlc"
                     /locus_tag="ACHKIR_001704"
                     /gene_synonym="dgsA"
     CDS             complement(21813..23033)
                     /gene="mlc"
                     /locus_tag="ACHKIR_001704"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar metabolism global transcriptional
                     regulator Mlc"
                     /protein_id="HFO9989454.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDSPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            complement(23168..24061)
                     /gene="ynfL"
                     /locus_tag="ACHKIR_001705"
     CDS             complement(23168..24061)
                     /gene="ynfL"
                     /locus_tag="ACHKIR_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO9989455.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMMDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPSVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYNL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPAARNFRIHLLNALR"
     gene            24168..25421
                     /gene="ynfM"
                     /locus_tag="ACHKIR_001706"
     CDS             24168..25421
                     /gene="ynfM"
                     /locus_tag="ACHKIR_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9989456.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYVGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHHRLHA"
     gene            complement(25471..25590)
                     /locus_tag="ACHKIR_001707"
     CDS             complement(25471..25590)
                     /locus_tag="ACHKIR_001707"
                     /inference="COORDINATES: protein motif:HMM:NF033695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KPN_01571 family protein"
                     /protein_id="HFO9989457.1"
                     /translation="MTHSKMNPFIWVLIILMTLDALRELAGASSILGWLLTLV"
     gene            25846..26154
                     /gene="asr"
                     /locus_tag="ACHKIR_001708"
     CDS             25846..26154
                     /gene="asr"
                     /locus_tag="ACHKIR_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance repetitive basic protein Asr"
                     /protein_id="HFO9989458.1"
                     /translation="MKKVLALVVAAAMGLSSAAFAAETATTPAPTATTTKAAPAKTTH
                     HKKQHKAAPAQKAQAAKKHHKNAKAEQKAPEQKAQAAKKHAKKHSHQQPAKPAAQPAA
                     "
     gene            26247..26330
                     /locus_tag="ACHKIR_001709"
     CDS             26247..26330
                     /locus_tag="ACHKIR_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9989459.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            26430..27251
                     /gene="ydgD"
                     /locus_tag="ACHKIR_001710"
     CDS             26430..27251
                     /gene="ydgD"
                     /locus_tag="ACHKIR_001710"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HFO9989460.1"
                     /translation="MRRTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKAAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(27290..27619)
                     /gene="mdtI"
                     /locus_tag="ACHKIR_001711"
     CDS             complement(27290..27619)
                     /gene="mdtI"
                     /locus_tag="ACHKIR_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HFO9989461.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(27606..27971)
                     /gene="mdtJ"
                     /locus_tag="ACHKIR_001712"
     CDS             complement(27606..27971)
                     /gene="mdtJ"
                     /locus_tag="ACHKIR_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015606 - spermidine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015848 - spermidine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HFO9989462.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            28382..29416
                     /gene="tqsA"
                     /locus_tag="ACHKIR_001713"
     CDS             28382..29416
                     /gene="tqsA"
                     /locus_tag="ACHKIR_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HFO9989463.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLQQYRNSIM
                     TPLKAIEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            complement(29441..30829)
                     /gene="pntB"
                     /locus_tag="ACHKIR_001714"
     CDS             complement(29441..30829)
                     /gene="pntB"
                     /locus_tag="ACHKIR_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HFO9989464.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATILGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(30840..32372)
                     /gene="pntA"
                     /locus_tag="ACHKIR_001715"
     CDS             complement(30840..32372)
                     /gene="pntA"
                     /locus_tag="ACHKIR_001715"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HFO9989465.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFTAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            32896..33840
                     /gene="ydgH"
                     /locus_tag="ACHKIR_001716"
     CDS             32896..33840
                     /gene="ydgH"
                     /locus_tag="ACHKIR_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YdgH"
                     /protein_id="HFO9989466.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            34026..35408
                     /gene="ydgI"
                     /locus_tag="ACHKIR_001717"
     CDS             34026..35408
                     /gene="ydgI"
                     /locus_tag="ACHKIR_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO9989467.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWS
                     ITGAGILLLTFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            35445..36167
                     /gene="folM"
                     /locus_tag="ACHKIR_001718"
     CDS             35445..36167
                     /gene="folM"
                     /locus_tag="ACHKIR_001718"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HFO9989468.1"
                     /translation="MGKAQPLPILITGGGRRIGLALAWHFINQKQPVIISYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKSTHGLRAILHNASAWMAEKPGTPLTDVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(36164..36499)
                     /gene="ydgC"
                     /locus_tag="ACHKIR_001719"
     CDS             complement(36164..36499)
                     /gene="ydgC"
                     /locus_tag="ACHKIR_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HFO9989469.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLASLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            36628..37347
                     /gene="rstA"
                     /locus_tag="ACHKIR_001720"
     CDS             36628..37347
                     /gene="rstA"
                     /locus_tag="ACHKIR_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HFO9989470.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDRAEETILQE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLSKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            37351..38652
                     /gene="rstB"
                     /locus_tag="ACHKIR_001721"
     CDS             37351..38652
                     /gene="rstB"
                     /locus_tag="ACHKIR_001721"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HFO9989471.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGVAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAVESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLAQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            38728..39657
                     /gene="tus"
                     /locus_tag="ACHKIR_001722"
     CDS             38728..39657
                     /gene="tus"
                     /locus_tag="ACHKIR_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006274 - DNA replication termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HFO9989472.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAELEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKTAVRLPGVLCYQV
                     DNLSQAALVSHIQHINQLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IATLPQDAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(39654..41057)
                     /gene="fumC"
                     /locus_tag="ACHKIR_001723"
     CDS             complement(39654..41057)
                     /gene="fumC"
                     /locus_tag="ACHKIR_001723"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HFO9989473.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRVFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGG"
     gene            complement(41200..42846)
                     /gene="fumB"
                     /locus_tag="ACHKIR_001724"
     CDS             complement(41200..42846)
                     /gene="fumB"
                     /locus_tag="ACHKIR_001724"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFO9989474.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            43045..44220
                     /gene="manA"
                     /locus_tag="ACHKIR_001725"
     CDS             43045..44220
                     /gene="manA"
                     /locus_tag="ACHKIR_001725"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009298 - GDP-mannose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HFO9989475.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESNKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     RNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPDAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISECYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLCKGSQQLQLKPGESAFIAANESPVTVRGHGRLARVYNKL"
     gene            44321..45829
                     /gene="ydgA"
                     /locus_tag="ACHKIR_001726"
     CDS             44321..45829
                     /gene="ydgA"
                     /locus_tag="ACHKIR_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFO9989476.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKVNPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMPLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(45874..47139)
                     /gene="uidC"
                     /locus_tag="ACHKIR_001727"
     CDS             complement(45874..47139)
                     /gene="uidC"
                     /locus_tag="ACHKIR_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HFO9989477.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQPADDEAGLRIRLKNELRRA
                     DKPSAGTGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRLTPQFTLTFAPIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSALGDRLNYWDVKMSIQYDFMLK"
BASE COUNT        11903 a        12133 c        11329 g        11799 t
ORIGIN      
        1 ttaaaatcag gcaactagct gtcaaatatt cagagaattt tactcactaa agtaagaaga
       61 agattaaaga gtcttaaaca tatttcagaa caatcagatt tataaatttg aaaattatta
      121 taacggaagg gtatacaaaa aaagtaattt tgctggcgac tgcgaaacgg cattcgcatt
      181 agcggtgaag tttgccctca cccagcggct gcaattactg ctataacgag gctggtctgg
      241 cgataaatcc aggccagcca ttggtggtgt ttatacgttc aagccacgat gttgcagcat
      301 cggcataatc tgaggtgcct taccgcgcca ttgtcgatac aggcgttcca gatcttcgct
      361 gttacctctg gaaagaatcg cctcgcgaaa acgccgccca ttttcacgcg ttaatccgcc
      421 ctgctcaaca aaccactggt aaccatcatc ggccaacatt tgcgtccaca gataagcgta
      481 ataacctgcg gcatatccgc caccaaaaat atgggcgaaa taactgctgc gatagcgtgg
      541 cggtatagca ggaagatcca tattttccgc caccagcgcc cgcaattcaa aatcatcgac
      601 atcctgcatt gcttcgtttt cttccaggca atgccagcgc atatcgagaa gtgcggcgct
      661 aagcagttcg ctcatctcat accctttatt gaacaggctg gcattacgca ttttctgttg
      721 cagttcatca ggcattgctg ccccgctctg ataatgccgg gcgtagcgag cgaatacctg
      781 cggatgcgtt gcccagtgtt cgttgatttg cgacggaaat tcgacaaaat cacgcggcgt
      841 gttggtgccg gaaagcgtgg cataacgctg gcgggcaaaa aggccgtgca gcgtatgacc
      901 aaattcatgg aataaggtta tgacatcatc ccagagtaac aacgcaggct ccccagcggc
      961 ggatttctga taattgcaga cgttataaat taccggatgc gtttcattca gcgttgattg
     1021 ctcaacaaaa ttgcccatcc atgcaccgcc gctttttgaa tcacgggcga agaaatcacc
     1081 gtaaaataac gccagcccca cgccattatg atcaaaaatt tcccacacac gaacgtcagg
     1141 atggtagaca ggaatatcaa aacgttcgac aaacttaata ccgaagagct gattcgcggt
     1201 ccagaataca ccttcattca acaccgtgtt taattcaaaa tatggcttga gctgcgattc
     1261 atcgagatcg aatttctcac gtcttacctg ctcggcataa aatgcccagt cccacggctg
     1321 cgcgctaaac ccgccctgct gcttatcaat aaccgcctgt atggagacta attcatcgct
     1381 cgcacgttga cgcgccgctg gaacaatttc ccgcataaag ttgagtgctg cttctggcgt
     1441 ttttgccatc tgatcggcga ttttccatgc ggcataatga gggaaaccaa gcagtttcgc
     1501 ctgctgcgcg cggatctcca ccagacgttg aatgatagcg cgggtatcat tggcatcatt
     1561 tttttccgct cgcgtccagc ccgcagtgaa cagtttttca cgcgtcgcgc gatcgcgcag
     1621 ttcagcaagc gctggttgct gggtggtatt cagcaacgga atcagccatt tgttatccag
     1681 acctttctcg cgagccgcct ctgccgccag cgcaatctct tgctcactca ttcctgccag
     1741 ctgcgcgaaa tcgttcacaa ccagaccgcc ggatttattt gctgccagta accgctggtt
     1801 aaactggctg gtcagggtcg cagcttctgt attcagtact tttaattttg ctttatcagc
     1861 ttgtgcaagt ttggctccgg caaggacaaa acgttgatga atgacctcca ccaggcggat
     1921 ggattcacta tcaagtccca gggattcacg gcgttgccag acagcatcta cccgcgcgaa
     1981 taattcaccg ttcagataga tatcattagc cagttccgcc agttcagcgg aaaactgttc
     2041 atcaagacgc tgtaattcat cattagtatg cgccgcagtc atcgcaaaaa agacgctggt
     2101 aacgcgggta agtaattctc cgctttgttc cagtgccaga atagtattgt tgaaatcagg
     2161 cgcttgcggg ttaagcgcga tggcagcaat ttctgcccgc ttttgctgca ttccctcatc
     2221 gaatgccggg cgatagtgat gattggcaat ttgatcaaaa tggggagcca gatacggcag
     2281 tgtgctttgc acaaggaaag gattcattgt tgtcattttc ttctcctgaa cgcgaggtgt
     2341 tccatagcgt aggcttactg ataacggagt gcaatcttgc aaaccagtat tacccgctct
     2401 taagcatcac gtgctatgtt attgacacac aaaagcgttg aggaacagtg agatgatcgt
     2461 tttagtaact ggagcaacgg caggttttgg tgaatgcatt actcgtcgtt ttattcaaca
     2521 agggcataaa gttatcgcca ctggccgtcg ccaggagcgg ttgcaggagt taaaagacga
     2581 actgggagat aatctgtata tcgcccaact ggacgttcgc aaccgcgccg ctattgaaga
     2641 gatgctggca tcgcttcctg ccgagtggtg caatattgat atcctggtaa ataatgccgg
     2701 cctggcgttg ggcatggagc ctgcgcataa agccagcgtt gaagactggg aaacgatgat
     2761 tgataccaac aacaaaggcc tggtatatat gacgcgcgcc gtcttaccgg gtatggttga
     2821 acgtaatcat ggtcatatta ttaacattgg ctcaacggca ggcagctggc cgtatgccgg
     2881 tggtaacgtt tacggtgcga cgaaagcgtt tgttcgtcag tttagcctga atctgcgtac
     2941 ggacctgcat ggtacggcgg tgcgcgtcac cgacatcgaa ccgggtctgg tgggtggcac
     3001 cgagttttcc aatgtccgct ttaaaggcga tgacagtaaa gcggaaaaaa cctatcaaaa
     3061 taccgttgca ttgacgccgg aagatgtcag cgaagccgtc tggtgggtgt caacgctgcc
     3121 tgctcacgtc aatatcaata ccctggaaat gatgccggtt acccaaagct atgccggact
     3181 gaatgtccac cgtcagtaat ttttataccc ggcgtaactg ccgggttatt gcttgtcaca
     3241 aaaaagtggt agactcatgc agttaactca ctcacaagca agaacgaatg accgtcgaaa
     3301 cgcaacttaa tcccacacag cctgtcaatc agcagattta tcgtattctt cgtcgcgaca
     3361 ttgtccattg cctgattgca ccaggcacac cgttgtcgga aaaagaagtt tctgttcgtt
     3421 tcaatgtgtc acgccagccg gttcgtgaag cctttattaa actggcggaa aacggcctga
     3481 ttcaaattcg tccgcagcgt ggcagctacg tcaacaaaat ttccatggcc caggtgcgca
     3541 acggcagttt tatccgtcag gccattgagt gcgcggtggc gcgtcgggcg gcgagcatga
     3601 ttaccgaaag ccagtgctac caactggaac aaaatcttca ccagcaacgc attgccatag
     3661 agcgcaagca actggatgat ttttttgaac ttgatgacaa cttccatcaa ctcctgacgc
     3721 agattgccga ctgtcaactg gcgtgggata ccattgagaa cctgaaagcg accgttgatc
     3781 gcgtgcgcta tatgagtttc gaccacgttt ctccaccaga aatgctgtta cgccagcatc
     3841 ttgagatttt ctctgccctg caaaaacgtg atggcgatgc ggtagaacgt gcaatgacgc
     3901 accatttgca ggaaatcagc gaatccgtgc gccagatccg tcaggaaaac agcgactggt
     3961 ttagcgaaga gtaattcatt tcctctcatc ccatccgggg tgagagtttt tccctccgtc
     4021 atatggctca tgcatgcatc aaaaaagatg tgagcttgat caaaaacaaa aaatatttca
     4081 ctcgacagga gtatttatag tgcgcccgtt acgtgggctt cgactgtaaa tcagaaagga
     4141 gaaaacacct atgacgacct acgatcgtaa ccgtaacgca atcaccactg gcagccgtgt
     4201 tatggttagc ggcaccggtc acactggcaa gatcctgtcg attgatactg aaggtctgac
     4261 cgctgagcaa atccgccgcg gaaaaaccgt agttgttgaa ggttgtgaag agaaactggc
     4321 acctctggac ctgattcgtc tcggcatgaa ctaagcgtgt gaatgccgcc gatggcggca
     4381 ttgctttttt tacttcacgg aatatttcgc cacggtcgct ttcgcaccat gcgctaataa
     4441 agacaagtac gcttccgtca cttttgcagt aaacaagcta ttgtctggca aatcatcacc
     4501 aaagatcgcc ttaatcgcca gcaatgactg gacgcgcgct ttcccttcgg cactactttg
     4561 tacagccttc tgaataacag gtaacagtgg gtcactgatt tctatcggat ttccctgttc
     4621 atcaacacca ccgacataac gcatccaacc tgcgacgccc agcgccagca gatcgaactt
     4681 gctgtcatgc gccagatgcc agcgaacaga atccaacatc cgctgtggca atttctggct
     4741 accatccatc gcaatctgcc aggttcgatg acgtagcgcc gggttgctat agcgttcaat
     4801 taatcggtta gcgtaatctt gcaaatcaac gccctgcact ttcaacgtcg gcgcttgttc
     4861 ctgcaacatc aaggcatacg ctgcatgacg ataatgttca tcttccatgc agtcattaat
     4921 gtgctgatat ccggcaagat accccagata cgccaggaat gaatgactgc cgttgagcat
     4981 gcgcaacttc atctcttcat aaggcagcac atcactaacc agttcggctc ccgctttttc
     5041 ccattccgga cgtccggcaa caaagttatc ttctattacc cactggcgga aaggttcaca
     5101 ggcaacgccc gcaggatcgc gcacaccggt aagttgttcg attttcgcca gcgtatcctc
     5161 cgtcactgcg ggcacaatac ggtccaccat tgttgatggg aaagtcacgt tttcttcgat
     5221 ccattgtgcc agttttacat caacagcttg cgcgtaggaa gtgacaacgt cacgcataac
     5281 atgaccgttt tctggcatgt tgtcacatga catgacggta aatgcgggaa gtcctgccgc
     5341 tttacggcga gccagcgcct cgacaatcac ccctgttgca gttttcggct ggtggggatt
     5401 ttgtacgtcg gcaacgacca tcgggtgatc gagcattaac tgtccggtcg ccggagagtg
     5461 gaaataccct ttttcggtga ttgtcagaga gacaatcgcg atttgcggtt cacacatcac
     5521 agccaacacg gtttctaagc catcaatctg tacgtgcaag gcttttttaa cgacgccaac
     5581 gacgcgagcc gtccacgcat cggccgacat ttccgcaacg gtataaagat tatcttgctg
     5641 ttgtaaatcg gcaatttgct gttcgccgcc gattaaattg acctcataat atccccagtc
     5701 actgaaatgt tccgtagcaa gaatatcggc atacacaccc tgatgcgcac ggtgaaatgc
     5761 accaaagcct aaatgaacaa ttcttggggc caggttatta cgatcataaa cagggagcgt
     5821 cgcttttgct gataacaaat tatttcccat aacaattcct taaatttaaa tatggcaagc
     5881 tgcatgtttt gttatatgaa taaaaaatcc cctctccggt aagagaaggg atggaggatt
     5941 tacagacttc ttgaaggttg cgcagctgtt acaacacgcg gttgatcttc cgcggcatct
     6001 tccagtgcac ttaaatcacg gtctttcact tctggcattt tcagcgcaga gattaaacca
     6061 atcactgaat atgccatgat cataatggcg atcggatacc aggattccgt catggtgcag
     6121 aaaatacccg ccaggatagg accaaaaccg gaagcgataa gaccaccaat ttctttagaa
     6181 atggccatcc gggtaaagcg gtttttacag ccgaacattt ctgccatggt aatgttttcc
     6241 agagcaaata atcccagcac cgcacagtta tgaatcacaa tcagtgccac cataatggtg
     6301 ctcggggcat agcttttatc tacgatgata gaaagcattg gccatgccag cacaatcgcg
     6361 gaggtattca taataatata cgggatccgg cgaccaattt tatcggataa ccaaccaagg
     6421 aacggaatgg tcataaagcc gagaatcgaa ctgatcatca atgcatctgt tggaattgct
     6481 ttgttaaaca ataacgtctg tactaaatag cctgcaagga aagtctgaat taaaccggag
     6541 ttaccagcct gaccaaaacg cagccctgtt gccagccaga aggatttgct ctggaacatg
     6601 ctaccagcag gtgcaggttt tgctgtcggt tggttgctgt cgttaacctt ctcaaagacc
     6661 gggctttctt tcagattcat acgcaaccag atagcaaaga ccatcacgac aacgctcgcc
     6721 aggaacggta tacgccatcc ccacgccaac agttcctctt tactgagaat gaagaacata
     6781 aaggcccaga ttgccgttgc gcttaaggtt ccgcagttag ttcccatagc cacaaatgag
     6841 gagataattc cgcgcttacc ttttggcgca tattccgcca gcatcgtacc ggcaccggaa
     6901 atttccgcac ctgcacccaa tccctgaata atacgcagcg tcaccagcaa gatgggtgca
     6961 aaaacaccaa tctgtgcata ggtcggtaac acaccaatta aggtggtaca gatccccatc
     7021 atggtgatgg taataaagag tactttttta cgccctattc tgtcgcccat tttgccgaaa
     7081 ataaatgctc cgacaatacg cgccacataa cctgcaccgt aggttcccat tgccagaatt
     7141 aacgccattg ccgttgatga ttcaggaaaa aatatttcat gaaacactaa cgctgcaccg
     7201 agcgaatata actggaaatc cataaattca agcgcagtgc ccagccatcc cgatactgct
     7261 gctttcacca aatccttagt gcttctttcg tgtttttcta ttgtcataat ggttatctct
     7321 aaaaaagagg taagatgcgt actacttact cgccgttatt ggtattattc agaaaaagta
     7381 agtaagactt tgcagcaatg tttttgatcc tgttcaaata aactaatggc atcagcaaca
     7441 tgctggaaat caaacgtatg ggtaattaat ttttctggtt taattaaccc tttacttaac
     7501 cagtcgataa cgaccggaaa tttatttgca tttaagcgtg aagagaaaat agagagttct
     7561 tttccggtaa ttccttgctg aatcacttca gacggttcac tggagaagcc catcaataca
     7621 atacgtgccg ctggagaagc cagcgttaca gcctctttca ggatagaagg atgacaagcc
     7681 gcatcgataa ttaatgtcgg cttgatgcct ttttcagcga aactctcgcc aagcggtgtc
     7741 tggctgttat taatcgccca gtctgccccg ctctctttcg ctttttccag tcgttcatca
     7801 atgcgatcgg caacaatcac atttttaacg ttatagacgc cttttaatac ctgaacgatc
     7861 gtcaggccga ttggaccggc accgtaaacc agaacggtat cattttcagt cggttgacca
     7921 tgaccggtaa cgttagccgc aatggtaaaa ggttcgatca tcaccgcata ttgatcggcc
     7981 actgcttcag gaattttcca cgcatttttt gccggaacca cggcatattc actgaaacca
     8041 ccgtcagcgt gcacacctaa tacagccagt gtcgtacaaa cgttcggctt acctatagag
     8101 cacggatagc aatgcccaca gctgaccacc ggatcgacag caacgcgttc accgactctg
     8161 gcgctttcca cgccttcacc cactgcatca atgacgccaa agaattcatg accaatgacg
     8221 cgcggatatt tcgcaaaagg attatgccca cgataaatat ggctatctga accacaaatt
     8281 ccggcaagtt tcacttttac tcgtacttca cccgctgacg gggtgggtat ttcgcgttcg
     8341 ataattgcca gttgattcgg tttttcaatt aaaatgcttt tcattatctt actccttacc
     8401 agttccacag cgtgccatct tccagacgtg cgactggcag ataagcaggt tcatagggat
     8461 atttcgccgc cagcttttca tcgaattcga tgccaagacc cggtttgtct cccggatgca
     8521 tatagccgtt atcgaaagtc cagttgtgcg ggaagacttc aagcatttgt tcggaataac
     8581 ccatgtactc ctggacaccg aaattgggga cccacagatc aaagtgcagc gccgcagcca
     8641 tgcagactgg cgacaaatcg gaaggaccgt gtgagccagt acgtacctga tacagcgaag
     8701 caaaatcggc aatccggcgc ataccggtaa tcccccccgc gtgagtcagc gtggtgcgga
     8761 tatagtcgat aagttgttct tcaatcagct gtttgcagtc ccagatgctg ttgaagactt
     8821 cacccactgc aattggcgtg acggtgtgtt ggcgaatcag gcggaaacac tcctggtttt
     8881 ccgcaggcgt cgggtcttcc atccagaaca tgcgataatc ttcaatgctt ttaccaaagc
     8941 gcgccgcttc aataggcgtt aagcgatggt gcatgtcatg cagcaaatgt tcattaaaac
     9001 caaacttgtt acgtaccgcg tcaaacaatt tcggcatgaa atcgaggtat ttctccgtcg
     9061 accacagctg ctcttccggc cactgtcctt tggttgcggg ttcataagcc agacctttac
     9121 ctttcgacat gccataggtg gttttcatac cagggattcc gcactgcacg cggatggctt
     9181 tgaatcccag ctcctgatga cgggcataat catccagagc ttcatcaata ctgtgaccgg
     9241 tggtatggca ataaaccatc accccttcac gagacgcgcc gccgagtaac tggtaaagcg
     9301 gcatgttggc agctttggct ttaatatccc acagcgccat atcaaccgct gaaatggccg
     9361 acatcgtaac cggaccgcga cgccagtaag cacctttata gaaaaactgc cagatatctt
     9421 cgatacggtg tgcatcgcga ccaataagct gcggacaaag gtgatcctgc aaataagagg
     9481 ccacggaaag ctcacgtcca ttgagggtgg catccccaag gcccgtaata ccgtcctcag
     9541 tggtgatttt taatgtgacg aaattacgcc ccggacaggt aacaaaaact tcagccttta
     9601 cgatcttcat gttcgattcc ttgcatcgct tgtcgtgatg catgaaatct acgcaactga
     9661 gctactacca tacaagtatg aagatcaaaa aaagccggag tgatcacaaa aaaaggcgta
     9721 tatttgcgcc gtgaatggtt gacaaaagat gaaatagaat accttttgtc agctgacact
     9781 tcctcgtatc ttattgataa aagggattta tgttcctacg tgcgccccca gcccgcaaca
     9841 atgatcaaca tgccgcaaag cgcaatcaac gcccccgtcc agtcataaag actcagtttc
     9901 acgccatcca caacgcgcag ccacatcaac gccgtgcaga cataaacgcc accataagcg
     9961 gcgtaaacac gcccactcgc cgcaggatgc aacgttaaca accagacaaa cagcgccagt
    10021 gaaatccccg ccggaagcaa cagccagata ctggcgtttc gttttaacca caaccagggc
    10081 agaaagcatc caataatttc acacagcgca gtagcaaaaa atagtaacgt tgttttaatc
    10141 atctttgtca cttattgaca tcatgtatag ttataggacg acataatatc atcaatataa
    10201 acatcctcct ggtacgtttt gcgttcgcag tggatggtgt agaatcacct ttaatcattc
    10261 atacagggaa tgaattatga aaattactct cagcaaacga attggcctgc tcgcttttct
    10321 gctgccttgc gcactggcat tgagcacaac tgttcatgcc gaaactaaca aactggtgat
    10381 tgagtctggc gacagtgcac aaagccgcca gcacgccgct atggaaaaag agcaatggaa
    10441 tgacacgcgc aatctgcgcc agaaagtgaa taaacgcact gaaaaagagt gggataaagc
    10501 cgacgccgct tttgataacc gcgataaatg tgagcaaagc gccaacatca atgcctactg
    10561 ggagccaaat actttgcgct gcctggaccg tcgaactggc cgcgttatta ccccctaacc
    10621 tgttattgat ttaaggaatg taaggacacg ttatgccaag cgcccacagt gttaagctac
    10681 gcccgctgga gcgtgaagat ttacgctatg tacatcaact cgacaataac gccagtgtga
    10741 tgcgttactg gtttgaggaa ccctacgaag cctttgttga actctctgat ctgtacgata
    10801 agcatattca cgatcagagc gaacggcgct ttgtggtgga atgtgacggc gaaaaagccg
    10861 gtctggtgga gctggtggaa attaaccatg ttcatcgccg cgcagaattt cagataatta
    10921 tctccccgga atatcagggg aaaggtctgg caacgcgtgc cgcaaaatta gcaatggact
    10981 atggcttcac cgttctcaat ctctataagc tgtatctgat cgtcgataaa gagaatgaaa
    11041 aagcgattca catttaccgc aagcttggct ttacggttga aggtgaattg atgcatgagt
    11101 tctttattaa tggtcaatat cgtaatgcca ttcgcatgtg catattccag catcagtatc
    11161 tggcagagca caaaacaccg ggtcagactc tcctgaagcc gaccgcacaa tagcattaat
    11221 aataatcgat agtatttttg atggtgtaaa cccgttcgac ggcgggtttt actccttcat
    11281 caacaatgat tagctgacag tccaccggat tggcgtgact gtcatattca cagctctgtt
    11341 ttacattacc aaccctttga ttattcagta aagtaaccgc tgtgtaatct aattttttga
    11401 tcggatccgt tgatggcgta gcgctgacag ataatgtttt gtcgttactt ttcgtggttt
    11461 tacccagcgg atacccctga tcatcatagc gatattccat ctgcatctgt ttgctgctgg
    11521 ctttaatcac aaagccatta tcatcggttt cccagctcac cccggcagaa ggtaattctg
    11581 ctagctggca ttttccctgc aaacgaactc tcttctccag tgtctgggca tcacggtaat
    11641 aattggcgtc cagtaccagg gcaaccacgg tattattttc cagatccagt aattcgagtg
    11701 aatcaaaaca gccttcttcc gacaaagtcc ccgaaacacg tttcgtcact tcaccttgct
    11761 catccattaa tgtctgagtg aaatctttta ccggaccacg cagcggatca aaatcgaatt
    11821 cattagagaa actggccatc tcaggggtaa atgaaagtgt tacctctgtg cggtcacatc
    11881 ctgtgaggaa tatcgcgagt aagcatggca gtaatttgta tttcacaaca gtcaccagag
    11941 agtagagatg attctcaatc atagtagcaa atacagtact ttacacgtta aatgctatgc
    12001 ttaaagaagt tatcttcgcg taaggagctt atgatgaaac tctcaacctg ctgtgccgcg
    12061 cttctgctcg ccctcgcctc acccgcggta ctcgccgcgc cgggatcctg tgagcgcata
    12121 cagagcgata tatcacagcg cattatcaat aatggcgtac ccgaaagcag cttcacgtta
    12181 agtattgtac ccaatgacca ggttgatcag cctgattccc aggtcgtcgg gcattgcgct
    12241 aatgatacgc ataaaattct ctacacccgc accaccagcg gtaacgtctc tgctcccgcg
    12301 cagtctaccc aggatggcgc gcctgccgaa ccgcagtaat acattatcga ctgcacgccg
    12361 gatacgacac atccggcgat ttgaacatac aacataatcc caccttatta ctcataccct
    12421 tctattgata tggattaata attcttaacc caaaataggt agactccctc tattgttagc
    12481 gcgctaaata ttcaatatat aaacttttat ataacgataa agaacaggga gtgagttatg
    12541 tccaaaaatg aacaaatggt gggcatcagc cgcagaacac tcgttaaatc taccgcgata
    12601 ggttctctgg cgctggctgc aggcggtttt tctttaccgt tcaccctgcg cagtgcagcg
    12661 gcagcggtac agcaggccag cgagaaagtg gtctggggtg cctgttccgt caactgtggt
    12721 agccgctgtg cacttcgtct gcatgttaaa gataatgaag tgacctgggt ggaaactgac
    12781 aacaccggca gcgatgagta tggcaatcat caagtacgcg cctgtttgcg cggtcgctcc
    12841 atccgtcggc gtattaatca tcccgaccgc ttgaattacc caatgaaacg cgtgggcaaa
    12901 cgcggcgaag gcaaattcga acggattagc tgggatgaag ccctggatac tatcgccagt
    12961 agcctgaaaa aaaccgtcga acaatatggc aatgaggctg tatatattca gtactcttca
    13021 gggatcgttg gcggcaatat gacccgctct tcgccatccg cctcggcggt caaacgcctg
    13081 atgaactgct acggcggttc actcaaccag tatggctcct acagcaccgc gcaaatctcc
    13141 tgtgccatgc cctacaccta cggcagtaat gatggcaaca gcaccacgga tattgaaaac
    13201 agcaagctgg tcgtgatgtt tggtaacaac ccggctgaaa cccgcatgag cggcggtggc
    13261 atcacttatc ttcttgaaaa agcgcgcgag aaatcgaacg caaaaatgat tgttatcgat
    13321 ccgcgatata ccgatacggc tgctggtcgt gaagatgaat ggcttcctat tcgcccgggc
    13381 accgatgccg cgctggttgc gggtattgcc tgggtattgg ttaacgaaaa tctcgttgat
    13441 caaccttttc tcgataaata ctgcgtcggc tatgacgaaa aaaccttacc cgcagatgca
    13501 cccaaaaatg gtcactataa agcctatatt cttggtgaag gtgatgataa aacagcgaaa
    13561 acgccgcagt gggcttcgca aattacaggt atcccggtag accgtatcat caaactggcg
    13621 cgtgaaattg gcacagcaaa acccgcttat atctgccagg gctgggggcc gcaacgccag
    13681 gcaaacggcg agctgacggc acgcgctatt gctatgttgc ctattttgac gggcaatgtc
    13741 ggcatcagcg gcggaaatag tggtgcgcgt gaatcgacct acaccattac catcgaacgc
    13801 ctgccggtgc tggataatcc ggtcaaaacg tcaatctcct gcttcagttg gacagatgct
    13861 atcgatcatg gcccgcaaat gacggcaatc cgcgacggcg tccggggcaa agataaactg
    13921 gatgtgccca ttaagttcat ctggaactac gcgggaaata ccctggttaa tcagcattct
    13981 gacatcaaca aaacgcatga aattctgcag gacgaatcga aatgcgaaat gattgtggtc
    14041 atcgaaaact ttatgacctc atcggcaaaa tatgccgaca ttctgctgcc agacctgatg
    14101 accgttgagc aggaagatat tattcctaac gactacgccg gtaacatggg atatctcatt
    14161 ttcctccagc ctgtcaccag cgaaaaattc gaacgcaaac caatttactg gatcctgagt
    14221 gaagtcgcga aacgtcttgg acctgacgtc tatcaaaagt tcactgaagg tcgcacgcag
    14281 gaacaatggt tacaacatct gtacgccaaa atgcttgcca aagatccggc gttaccgtct
    14341 tacgacgaat taaaaaaaat gggtatctat aagcgtaaag atcccaatgg tcattttgtc
    14401 gcctacaaag catttcgtga cgaccccgag gcaaatccac ttaaaacgcc ttccggtaag
    14461 attgaaattt attccagcaa gctggcggaa attgcccgta cctgggaact ggaaaaagat
    14521 gaagtgataa gcccactgcc ggtttatgcc tcaacctttg aaggctggga ctcccctgaa
    14581 cgtagtacct ttccactgca actgtttggt ttccattaca aatcccgtac tcactcgacc
    14641 tacggcaaca ttgatgtcct gaaggctgcc tgccgtcagg aggtgtggat caaccctata
    14701 gacgcacaga aacgtgggat tgccaacggc gatatggtgc gggtgtataa ccatcgtggc
    14761 gaagttcggc taccagccaa agtgacacca cgcattctcc ctggggttag cgctatgggc
    14821 cagggagcct ggcacgaggc caatatgtct ggcgataaaa tcgaccatgg cggctgtgtg
    14881 aatacgctaa ccactctgcg cccttcacca ctggcgaagg gaaacccgca gcacactaat
    14941 ctggtcgaga tcgaaaaaat ataacccact acaaccataa attctggcat gacattttgt
    15001 ttgaaaagca ataagtgagt aatgatgaaa atccatacca cagaggcgct catgaaggct
    15061 gaaatcagcc gtagaagtct gatgaaaacc tccgcacttg gcagtctcgc gctggcaagc
    15121 agtgctttca ctctgccatt ttcccaaatg gtccgagcgg cagagacacc ggtagaagag
    15181 aaagcggtct ggagttcctg caccgttaac tgcgggagcc gctgtctgtt acgtttgcat
    15241 gtgaaagatg acaccgtgta ctgggtggag tctgatacga caggagacga cgtttacggt
    15301 aatcatcagg ttcgggcatg tttacgcggg cgctctatcc gccgacggat gaatcatcct
    15361 gataggttga aatatcccat gaagcgcgtc ggcaagcgcg gtgaaggtaa atttgaacgg
    15421 ataagttggg atgaagccct ggataccatc agtgataatc ttcggcggat ccttaaagat
    15481 tacggcaatg aggctgtaca tgtcctgtac ggaacaggcg tagatggcgg aaacatcacc
    15541 aactcgaacg tcccgtaccg tctgatgaac tcttgcggtg gttttctcag tcgctatggc
    15601 agctacagta ccgctcaaat cagtgccgca atgagttata tgttcggtgc caatgatggc
    15661 aacagcccgg atgatatcgc caatacgaaa ctggtcgtta tgttcggaaa taacccggca
    15721 gaaacccgga tgagcggcgg tggtgtcact tactacgtcg agcaagcccg agaacgttca
    15781 aacgcacgca tgatcgtcat cgatccacgt tataacgaca cagctgccgg gcgtgaagat
    15841 gaatggctgc caattcgccc aggcaccgat ggcgcactgg cctgtgcgat tgcctgggta
    15901 ctgattactg aaaacatggt cgatcagcca tttctcgaca aatattgtgt tggttacgat
    15961 gaaaaaacgc tgcccgccaa cgcaccacgt aacgcgcatt ataaagccta tattctgggc
    16021 gaagggcctg acggcatagc taaaacgccg gaatgggcag caaaaatcac cagcatcccg
    16081 gcagaaaaaa ttatccagtt ggcacgagag atcggttcag caaaacctgc ttatatttgt
    16141 cagggttggg ggccacaacg acactccaac ggcgaacaaa cgtcccgcgc tattgccatg
    16201 ctttccgttc tcaccggtaa cgtcggcata aacggcggca actcaggtgt acgcgaaggt
    16261 agctgggatc tgggggtaga atggttcccg atgcttgaga atcctgttaa aacgcagatt
    16321 tccgtcttta catggacaga tgccatcgac cacggtacgg aaatgaccgc gacccgtgat
    16381 ggtgttcgtg gaaaagaaaa actggatgtc cccatcaagt ttttatggtg ctacgccagt
    16441 aacacattga tcaatcaaca tggcgacatc aatcacaccc atgaggtgct tcaggatgac
    16501 agcaagtgcg agatgattgt tggcattgac cacttcatga ctgcctcggc taagtattgc
    16561 gatatcctgt tgcccgacct gatgccaaca gagcaagaag accttatctc tcacgaatct
    16621 gccgggaata tgggctatgt gatcctcgcc caacccgcaa cctcagcaaa atttgaacgc
    16681 aaacccatct actggatgct gagcgaagtt gccaaacgct taggaccaga cgtttatcaa
    16741 acctttactg aaggccgcag tcagcacgaa tggatcaaat atctccatgc gaaaacgaag
    16801 gaacgtaacc ctgagatgcc cgactacgag gagatgaaaa cgaccgggat ctttaagaaa
    16861 aaatgcccgg aagaacacta cgtcgctttc cgcgcattcc gtgaagatcc acaggcaaac
    16921 ccgttgaaaa caccttcggg gaaaatcgaa atttattctg aacgactggc gacgattgcg
    16981 gatacctggg aattgaaaaa agatgaaatt attcatcccc ttcctgcgta taccccaggt
    17041 tttgatggct gggacgatcc cctgcggaaa acctatccac tgcagctaac gggcttccac
    17101 tacaaagcgc gtacccactc cagctacggc aatattgatg tgttacagca ggcctgccca
    17161 caagaggtgt ggatcaaccc tattgatgct caggcacgcg gtatccgtca tggcgatacc
    17221 gtgcgggtat ttaacaataa tggagaaatg ctgattgccg caaaagtgac tccgcgtatt
    17281 ctgcctggcg ttaccgccat cgggcaaggt gcgtggctta aagcggatat gtttggtgac
    17341 cgggtcgatc acggcggcag tatcaatatt ctgacctctc accgcccttc accgctggca
    17401 aagggaaacc cgtcgcacag caatcttgtc cagatcgaaa aggtttaagg agtagcccat
    17461 gaccacacaa tatggatttt ttatcgattc cagccgctgt actggctgta aaacctgcga
    17521 actggcgtgc aaagatttta aagatcttgg cccggaagtc agtttccgcc gtatttacga
    17581 atatgctggc ggcgactggc aggaagataa tggcgtctgg caccaaaacg tgttcgctta
    17641 ttatctctcc atctcctgta accattgcga tgaccccgca tgtacaaaag tctgccccag
    17701 cggcgcaatg cataagcgtg aagatggctt tgtggtggtt gatgaggatg tttgtatcgg
    17761 ctgccgctac tgccacatgg cttgtccgta cggcgcgcca cagtacaatg ctgaaaaagg
    17821 gcacatgacg aagtgcgatg gttgttattc gcgcgtcgcc gaggggaaac aacccatatg
    17881 tgtcgaatcc tgcccgctgc gtgcacttga gttcggtcca attgaagagc tgcgccagaa
    17941 gcacggcact ctggctgccg tcgcgccact accgcgcgcg catttcacaa aacccaatat
    18001 cgttatcaaa cctaacgcca acagccgccc gaccggcgat accactggtt atctggctaa
    18061 tccggaggaa gtgtaaaatg ggaaatggat ggcatgaatg gccactggtt atctttaccg
    18121 tattgggtca atgcgtagta ggcgcgctga ttgttagcgg aattggctgg ttcgccgcca
    18181 ataatgacgc agaccgtcag cgtattgtcc gtggcatgtt tttcctctgg ctgttgatgg
    18241 gcatcgggtt tattgcctct gtcatgcacc tgggatcgcc actgcgagcg tttaactcgc
    18301 tgaatcgaat cggagcttct ggtctgagta acgagattgc tgccggttcc atattttttg
    18361 ccgttggcgg attatggtgg ctggtggcag tcatcggtaa aatgccgcaa gcgttgggga
    18421 aactctggtt acttgtcagc atggcgctcg gcgtcatttt cgtctggatg atgacctgcg
    18481 tgtatcaaat cgacacagtg ccaacctggt ataacggtta cactacgctg gctttcttcc
    18541 tgacagtatt gttgagcggt ccaatacttg ctgccacaat tctgcgggca gcccgcgtta
    18601 catttaatac cacaccattt gccatcatta gcgttctggc gctgatcgct tgtgccgggg
    18661 tgattgtgct gcaaggtttg tctctggcgt ctattcactc atccgtacaa caagccagcg
    18721 cactggtacc agattacgcc tccttacagg tctggcgtgt ggtattgtta tgcacaggtc
    18781 taggatgctg gctttgtccg ctcattcgtc gccgtgaacc tcacgttgcc ggacttattc
    18841 tggggctaat cctcatactt ggcggtgaga tgatcgggcg tgtgctcttt tatggcctgc
    18901 acatgacggt aggaatggct attgcaggtt aacaaattgg tgcggggcaa cccgcacatt
    18961 tcaggatgtt aggaatgacc catttttcac agcaagataa tttttctgtc gcggcgcgcg
    19021 tgttgggcgc gctgttttat tacgctccag agagtgcgga agccgcacct ctggttgcgg
    19081 tactcacccg tgacggctgg gaaactcagt ggcctctacc agaagcgtca ttagcgccgc
    19141 tggtgacggc ttttcagaca cagtgtgaag agactcacgc ccaggcctgg cagcgtttgt
    19201 ttgtcggccc gtgggcactg ccgtctccgc cgtggggatc ggtctggctg gatcgcgaat
    19261 ctgtgctgtt tggcgattca acattggcac ttcgtcagtg gatgcgcgag aaaggcattc
    19321 agtttgaaat gaagcaaaac gaaccggaag atcattttgg ttcactgctg ttgatggctg
    19381 cgtggttggc ggagaatggt cgccagacag aatgcgaaga attgctggca tggcaccttt
    19441 ttccgtggtc aacacgattt cttgacgttt ttatcgaaaa agcagaacac cctttctacc
    19501 gtgcactggg ggaactggct cgcttaacgc tggcgcaatg gcaatcacaa ctgttgattc
    19561 ctgtcgcggt taaaccactc tttcgatagg atcaccgtaa tattgccgga tggtggcgat
    19621 tacgcgcaat tcggcaataa taggttacag tgtcacgttt ttttatctct taaagcacgc
    19681 actgcttttg cggctggcct cttttgccgc aaaatagtcg cccgtgtttc attgcccatt
    19741 tctgctcatg catcatctac acatctatcc ggatctgcgc actatgttcc gccgtctgct
    19801 tatcgcaaca gtcgtcggta ttcttgcggc ctttgccgtt gccgggtttc gtcatgcaat
    19861 gctgctactg gagtggttgt tcctcaataa tgactcgggc agtctggtca atgcagcgac
    19921 aaatctttcc ccatggcgac ggctgctaac tccggcgctc ggcggactgg cggcgggttt
    19981 gttgctgatg ggctggcaga aatttaccca gcaacgccct catgcgccga ccgattacat
    20041 ggaagcgttg caaaccgatg ggcagtttga ttacgcagca agcctggtta aatcgcttgc
    20101 ctctctgctg gtagtaacca gcggcagtgc aattggtcgc gaaggtgcga tgattctttt
    20161 agctgccctt gccgcctcct gttttgccca acgttttacg ccacgccagg agtggaaatt
    20221 atggatcgcc tgtggagctg cggcgggaat ggctgcggcc tatcgcgccc cgcttgctgg
    20281 cagtttattt atagccgaag tgctgtttgg cactatgatg ttggcctctc tcggcccggt
    20341 gattatttcc gccgtcgtgg cgttgctggt tagcaatctg attaatcata gcgacgcgtt
    20401 actctacagc gtacaactct cagtgacggt tcaggctcgt gactatgcgc tgattatcag
    20461 tacaggtgtg ctggcaggtc tgtgcggacc actgttgtta acgttaatga acgcctgtca
    20521 tcgtggattt gtaagtctca aacttgcgcc gccctggcaa ctggcactgg gcgggttgat
    20581 cgtgggtctg ctttccctgt tcacacctgc agtgtggggc aacggctata gcaccgtaca
    20641 atccttttta accgccccgc cactgttaat gatcattgcc gggatcttcc tttgtaaact
    20701 gtgtgccgtg ctggcgagta gcggttccgg cgcacccggt ggtgtcttta caccgacgct
    20761 atttatcggt cttgccattg gcatgttgta tggtcgtagc ctgggattat ggttccctga
    20821 tggcgaagaa attacgcttt tactcggatt gaccgggatg gcgacactgt tggcggcaac
    20881 cacgcacgcg ccgattatgt cgacgttgat gatatgtgaa atgaccgggg agtatcagct
    20941 actccccggt ttattgattg cctgcgtaat tgcgtcggta atttcgcgga cgttacaccg
    21001 tgactctatc taccgccagc acactgcgaa gcatagctaa gcggatgtat tgccccagtt
    21061 cacgctgttc ggcgcgcggc agataaggca gttcaccaat gagcggtgcc ggaagttttt
    21121 taccgagcac atcaatgatt tccgcataat gcgccagtcc tggattgatt cggttagcca
    21181 cccagccaat gagcggcagc ccgtcgttgg caatcgcctg agctgttagt agtgcatggt
    21241 taatgcaacc ttcctgaata ccgacaacca tcaataccgg cagttgttcc tgcaccaccc
    21301 attcagagag cggacgcaaa tcattcatca gactgcgcca gccgccagtc ccttctacca
    21361 cgacatgatc gactttatcg gtcaggtttg ccaggccgtt tgaaatgagg gtgtaattga
    21421 ttgggcaact gtgcgccacg ctactttctt cttcgcttaa cgcgatagga ttaactgctt
    21481 cataaggcag ttcgatggtt gaaacactct gcaacaccag ggcatcttta ttacgcagcc
    21541 cttcgggtgt ctctttgctc cccttcgcta cgggtttata tcccgcaacc gtttttccct
    21601 gggaggctaa cgcttgtagc aatgcgcggg aaaccaccgt tttccctaca gaagtgtctg
    21661 taccggtaat aaagaaacgc ttcagcatca ctaactccac cgttatgctt cacaaatata
    21721 aaccaggaaa ataattaacc ttgaaagtct aagttatgct ttcctggccc aaattgagat
    21781 agcgcaaatt ttggtagaac agttaaaaaa tgttaaccct gcaacagacg aatcaacaaa
    21841 gaaccgttat acatcgcgtc ttttaccagc gcagcccctg ccatcgtacc ctggttagaa
    21901 aactgagtac tctcaacgct aatgtgctga ctatacgcag gaagggcctg ctgacggatg
    21961 ctgtccgaga tgaccgggaa gaggatatct gccgctttac ttaacggtga gccaatcagt
    22021 attttttgtg ggttaaataa attcaccatg atggcaagaa tgcgccctac atgcgcgccc
    22081 accccggtaa tgatgtcttt tgccagtaga tcgccgcgca atgccgcctg acacaatgaa
    22141 tccacggtta acggctgtcc atgtaacatc gagctcatgg attgattgag acgcagctgt
    22201 gccagctcaa gaatactgtc cacactggcg atggtttcga ggcagccgtg attcccgcaa
    22261 taacagcgtt tcccatacgg gtcgacctgc gtgtgaccta tttccacgag gctactgctg
    22321 cctgcgtgta gcagatgacc atcggtaatg acgcccgccc ccacgttgtg atcgataacc
    22381 acctgaatca catcgcgcgc cccgcgtgag gcaccaaaca aggcctcagc catcgtccat
    22441 gcgctgatat catgctgaat ataaaccggt acgccggtat gctgctccag cgcctcgccg
    22501 agcggcatct cttttacatc ctcgtagaac ggcatgcgat gtacaatacc attttccgta
    22561 tcaataattc ccggcaaggt tatggcaatc gaagtcagac gctcaagttt tttctggtgg
    22621 cggataaaaa actgatcgat atgggaaatg atacgatcca gcaatggcga gtcatctttt
    22681 aacgccagtt cctgcgactc ctccactacc agtttgctgc tcagatcgcg caaagcaagg
    22741 aaaatctccc cgcgactaat gcgcagagaa agatagtgcc aggcttcagt ttcaaccacc
    22801 agccccactg ccggacggcc acggttcccc gcttctttga tttccagctc ttgcaccagg
    22861 tgtgcttcga gcatctcacg gacaatttta gtgatactgg caggagccag ctgcgccaga
    22921 cgggaaagat cgatacgcga gactggacca agctgatcaa tcaggcgata aaccgcgccc
    22981 gcgttggtct gctttatttg atcaatgtgc ccaggctgat tttcagcaac caccgcatac
    23041 tccctatatt ttcgcgctcc gaaataatct gtaggctatg gtgaagcact tcaatacgtg
    23101 tcgtcaaatt tttacttagg catgtgatta acagcacatt tttcgggctt tttcgctgaa
    23161 atttccctca cctgagagca ttcagcagat gaatacgaaa gttacgcgca gccggacttt
    23221 gttcatggtg tttcggccag accaaccaca tttcagaaac cgcatcctct tcagcaatcg
    23281 gcacccagcg catttcgttg agctgaacac gtttaaatga cgcaggcaaa attgaaacac
    23341 ccagaccagc ggaaaccaga ccgatgatgg tcattgcctc acccacctcc tgagtgatga
    23401 cgggcgtcaa attgtaacgt cgcatcagcc cgagaatatc gtcatacagc cctgtcccta
    23461 cgtgcggatc aaaaaagaca aagggttctt tcgccagttc agccagcgtt acactcgggt
    23521 tatttgccaa gggatgatcg tgcgggatca tcgccataag cggttcatgg acgatgactg
    23581 cgtgttcaag cgtctccggt aacgctgtgt tacgcagcaa tcccatatcc agcgttcctt
    23641 caatgagcgg agcgatttgc tcgcgggtgt tcatttcgcg ggtttgtaaa tggacatcgg
    23701 gataatcacg gcgaaacagc gataacgtat cggacaccgc ccgaataaaa ggagcggacg
    23761 aagtaaaacc aatgcgcaac tcccccgctt caccctgatg cagtctttca gcgcgagcgg
    23821 cagcgtcatc catcatagac aggatttgcc gactatctgc aagaaactgt tttcctgctg
    23881 ccgtcagcaa tacactgcga ttagttcgtg ccagcagtcg ggcaccaatt tgttgctcca
    23941 gcgcctgaat ctgctgactt agcggcggtt gcgaaatatt caggcgggca gcggcgcgcc
    24001 cgaaatgcag ctcttctgca acagcaacaa agtaacgcag atgacgaagt tcaatattca
    24061 tatttaaaac atcttatttg agattattaa tatattagac agaacaattc gattttccta
    24121 atctattcat aagcctgaac cacaggcata tttagcaagg atttcaagtg agccgtacta
    24181 caactgttga tggcgctccg gcaagcgaca ctgacaagca aagcatttct cagccaaatc
    24241 aatttattaa acgcggtacg ccgcaattta tgcgcgtcac cctggcgctg ttctctgccg
    24301 gactggcaac cttcgcactt ctctattgtg tgcaacctat ccttccggtg ctttcgcagg
    24361 agtttggctt aacgcccgcg aacagtagta tttcactgtc catttctacg gcgatgttgg
    24421 ctatcggttt gctgtttact ggcccgttat ccgatgccat tggtcgcaaa ccagtgatgg
    24481 tcacggcgct actgttggcc tccatttgta cgttactttc gacaatgatg accagctggc
    24541 acggcatttt gattatgcgc gccttgattg ggctttcgtt aagtggcgta gcagctgttg
    24601 gcatgactta tcttagcgag gaaatccacc ccagtttcgt ggccttttcg atggggttgt
    24661 atatcagcgg caactcaatt ggcggcatga gcggacgctt aattagcggt gtcttcacgg
    24721 actttttcaa ctggcgaatt gcgctggcgg caatcggttg tttcgcgctg gcctcggcgt
    24781 taatgttctg gaaaatcctc cctgaatcac gccattttcg cccgacttca ctgcgcccta
    24841 agacgttatt catcaacttt cgtctgcact ggcgtgaccg gggattaccg ttattgttcg
    24901 cagaaggctt tttgctgatg gggtcgttcg tcacgctgtt taattacgtc ggctatcggt
    24961 tgatgctctc cccctggcat gtcagccagg ccgtggttgg cttattatcg ctggcttatt
    25021 tgaccggtac atggagctca cccaaagccg gaaccatgac cacccgctat gggcgcggtc
    25081 cggtgatgtt gttttcgacg ggggttatgc tgtttggttt attgatgacc ttattcagct
    25141 cgctgtggct gatctttgcc ggaatgttac tcttctcagc cggattcttc gcagcccact
    25201 cagtagccag cagctggatc ggcccccgcg caaaacgcgc taaaggccag gcctcctcgc
    25261 tgtatctgtt cagttactat ctggggtcga gtattgccgg gacgctgggt ggtgttttct
    25321 ggcataacta tggctggaac ggcgtcggcg catttattgc tctgatgctg gtcattgctc
    25381 tgctggtcgg gacgcgtttg catcatcgtc tgcacgcctg aaaaaataag tccggactga
    25441 ggtaaatacc cgtccggact tattgcctgc tcaaaccaac gttaatagcc atcctaaaat
    25501 agacgaagcg ccagccaatt cccgcagcgc atctagtgtc atcaggatta taagtaccca
    25561 aataaacgga ttcattttgc tgtgtgtcat ttattactga tgcgcagtta ttctaccgct
    25621 ttgtaagtag taaaatagtt aacacgatca agactactat tattggtaga tgaattttcc
    25681 ttaagtcaca atacgttatt atcaacgctg taatttattc agcgtttgta catatcgtta
    25741 cacgctgaaa ccaaccactc acggaagtct gccattctca gggatatagt tatttcaacg
    25801 gccccgcagt ggggttaaat gaaaaaacaa attgagggta tgacaatgaa aaaagtatta
    25861 gctctggttg ttgccgctgc tatgggtctg tcttctgctg cctttgctgc agagactgcg
    25921 accacacctg ctccgactgc gacgaccacc aaagcagcgc ctgcgaaaac cacacatcat
    25981 aaaaaacagc ataaagcagc acctgcccag aaagcgcagg cggctaaaaa gcatcataaa
    26041 aatgccaaag ctgaacagaa agctcctgaa caaaaagcgc aggcagcgaa gaaacacgcc
    26101 aagaaacaca gccatcagca accggcaaaa cctgctgcac aacccgcagc gtaagttttc
    26161 aacagtaatg ctggcgcgcc ccctcgcgcc tgaaaattac ggtgctaaac gggtaacgtt
    26221 tagcaccgcc tttagcggag ggtagtatgg tgggccgtta tcgctttgag ttcattctta
    26281 tcatccttat tttatgcgca ctgattgccg cccgttttta tctttcctga tcgtagttat
    26341 ctgattttac tcccactttc atcccgtccc gtctatagta tttatgaggg tttgctttta
    26401 ataatcataa ttacccacca gagtgtgata tgcgtagaac cattgctgta gtgttgggtg
    26461 caattagttt gacgtctgct tttgtgtttg cagataaacc agacgttgcc aaatcggcaa
    26521 acgatgaggt cagcaccctg ttttttggtc atgatgatcg tgtgccagtg aatgacacga
    26581 cccaatcacc gtgggatgcg gttggccaac tggaaacggc cagcggcaat ttatgtacag
    26641 cgacgctgat tgcacccaat ctggcattaa cggcaggaca ctgtttattg acgccgccaa
    26701 agggtaaagc cgataaagcg gtggcgctgc gttttgtgtc aaataaaggt ctttggcgct
    26761 atgagatcca cgacatagaa ggccgcgttg acccgacact gggaaagcgg ttaaaagcag
    26821 atggggatgg ttggattgtt cctcccgcag ctgcgccgtg ggacttcggt ttgattgtgc
    26881 tacgtaatcc cccttctggc attacaccat tgccgttatt tgagggagat aaagccgcgc
    26941 ttaccgccgc attaaaagcg gcaggtcgta aagtgactca ggcgggatac cctgaagatc
    27001 atctcgatac gttgtacagt catcaaaact gtgaagtgac tggctgggcg caaacgtcgg
    27061 tgatgtcgca tcagtgcgat accttgccgg gtgacagcgg ttcgcctctg atgttgcata
    27121 ccgatgacgg ctggcaatta attggggtgc aaagttcggc tcctgccgcg aaagatcgct
    27181 ggcgcgccga taaccgggcc atttctgtta ccggttttcg cgacaagctg gatcaactgt
    27241 cgcaaaaata atgttcaaac gctgcccgac agcgcgggca gcatgttcat caggcaagtt
    27301 tcaccatgat cattccagcc aacagcagca ccaggccaat ccagccttta cgatttaacc
    27361 gttgaccaaa caagatccaa cctgcggcta acgtggcggc aataccaaac ccgccccaca
    27421 atgcataagc gacagacaag tcgatccctt taacggcctg agaaagcgca ctaaaggcag
    27481 ccagcaccgc cgccagagag agcaagccaa atattttgcg acgaaagccg tcagaaaatt
    27541 tcaaaaagac gttagcaacg atttccagca cgattgccaa tgccagccag gcggcgtgaa
    27601 cccattcaaa ctgcgccatg gttcacctcc agttcaggtt tacgcgcttt acgggtacct
    27661 gatttgatca acacaatccc ggcgaccagg gtggttaacc cggcaatttt catcagagat
    27721 aaactttcgt cgaataacaa aacgctaaac aaggtaataa ataaaatacc gataccttcc
    27781 cacagcgcat aagctacgcc taaggcgatc tttttaacgg cgaaagagag aaatatatac
    27841 gacagagaaa tcatcaccag cattaaaata aagccgccat ttccctcact gacgctcgcc
    27901 catttcatgg acagcgtacc ggtaatttct gtagcaatag ccagacctaa taaaatccaa
    27961 taaatataca ttgtccttct cctgcaagag aattatttta attttcgctt aattcagcga
    28021 aaccaaagta aagtggtcaa gcccgcgctt agcgcaagtt ttggcagaag aaaggactaa
    28081 agcgcgttgc gccagtgctg ctcacctacg agcaagatag tggatgaggt acgaagatgg
    28141 gtaaatgtag aaaataacgt cctgaacaaa ttgtccataa tattacaatt atccgcagtg
    28201 ttgcttctcg tcatcgcgga tgataattgt cctcggtagt tgaacacgcc tgatttgtat
    28261 catagcttaa gaattaactc aaaatatttt cacttcttta cctgagcggt ttgattttcg
    28321 ttatgatgac ggagcgaaaa agacattatt attagcaaag gaagaaaaaa cggggacaag
    28381 catggcaaag ccgatcatca cgctcaatgg cctaaaaatc gtcattatgt tgggaatgct
    28441 ggtcattatt ctctgcggta tccgttttgc cgccgagatc atcgtgccgt ttattctcgc
    28501 attatttatt gctgttattc ttaacccgct ggtgcaacac atggtccgct ggcgtgtgcc
    28561 gcgtgtactg gcagtgtcaa ttttgatgac catcatcgtg atggcgatgg tgttgctgtt
    28621 agcttatctg ggttccgcgc tcaatgagtt gacgcggacg ttacagcaat atcgcaactc
    28681 tattatgacg ccactgaaag cgattgaacc gttgttgcaa cgcgtaggga ttgatgtctc
    28741 agttgaccaa ctggcgcatt acattgatcc gaacgcggcg atgacgttgc tcaccaactt
    28801 attgacgcag ttatctaatg ccatgtcatc aatcttttta ttgctgctga cggtgctgtt
    28861 tatgctgctc gaagtgccac aattgcccgg aaaatttcag caaatgatgg cgcgtccggt
    28921 tgaagggatg gcggcgattc agcgtgcgat tgacagtgtg tctcattatc tggtgctgaa
    28981 aacagccatc agcatcatca ccggcctggt cgcatgggcg atgctcgccg cactcgatgt
    29041 tcgcttcgct tttgtctggg gattgctggc ctttgcgctt aattacatcc cgaatattgg
    29101 ttcagttctc gcggcaatcc cccctattgc tcaggtactg gtgtttaatg gcttctacga
    29161 agcgttgctg gtgctggcgg gatatctgct gattaatctg gtcttcggca atattctgga
    29221 gccgcgcatc atgggacgtg ggctggggct ttcgacattg gtggtatttt tgtcgttgat
    29281 tttttgggga tggttgttag gaccggtggg tatgctgctt tccgtgccgt tgacaattat
    29341 tgtcaaaatt gcgcttgaac aaacagcggg aggtcaaagc atcgccgttc tgttaagcga
    29401 tctcaataaa gagtgacggc ctccgcagag gccgtcaggg ttacagagct ttcaggattg
    29461 catccacgct ggctttggcg tcaccaaaca gcatgtgggt gttttccttg aagaacagcg
    29521 ggttttgcac accagcatag ccagtgttca tcgaacgttt aaagacaatc acgttctgcg
    29581 ctttccacac ttccagcaca ggcataccag caataggact cttcggatca tcctgcgccg
    29641 ccgggttaac cgtatcgtta gcaccaatca ccagtacggt atcggtatca gcaaagtcat
    29701 cgttgatctc gtccatttcc agcacgatgt catacggtac ttttgcttca gccagcaata
    29761 cgttcatatg tccaggcaaa cgccccgcaa ccgggtggat accgaaacgc acgttgatac
    29821 cacgagcgcg cagtttctcg gtaatttcag cgacaggata ttgcgcctgc gcgactgcca
    29881 tgccgtaccc cggagtaatg atcactgaat gggagttttt cagcagttcc gctgtctctt
    29941 ctgcggtgat ttcgcggtgc tcgcccactt cctgatcatc gccagtagaa gagccgtcgg
    30001 tgccgaaacc acccgcaata acgctgataa aggaacggtt catcgcctta cacataatgt
    30061 aagaaaggat agcgcccgaa gaaccgacca gcgcaccggt cacaatcagc agatcgttgc
    30121 tgagcataaa gcccgcagcc gcagccgccc agccggagta cgagttcagc atcgacacca
    30181 ccactggcat atctgcacca ccgatggagg cgactaaatg ccagccgaat accagcgcaa
    30241 tggcggtcat tatcagcaat gccagcactt gcaggccgac gctgtccgtg cgaacaaata
    30301 caatcagcag caggaaggaa acgaccagag ccgccaggtt cattttgtga cggtttggca
    30361 gcatcaacgg tttagacgaa atcttgccac acagtttgcc gaacgccacc accgaacccg
    30421 tgaacgttac cgccccgatg aagataccga ggaacacttc cgtcaggtga atattgacca
    30481 gaatcggtgc cattcccgcg tcatgatgca gatagctgtt aaagccaacc agcactgccg
    30541 ccagacccac gaagctgtgc aggatcgcca ccagttctgg catttcggtc atttcaactt
    30601 tcttcgccag ccggatacca attgccccac caatgaccat cgccagcaag atccagccaa
    30661 cattacccgt atccggtccc aaaatggttg cgattaacgc aatcgccatc ccggcgatac
    30721 cgaagttgtt accctggcga gacgtttcat gtttcgaaag accggccaga ctgaagataa
    30781 acaggatcgc ggcaacaatg tatgcagctg taactaatcc tccagacata tgttacccct
    30841 taatttttgc ggaacatttt cagcatgcgc tgagtcacgg tgaagccacc gaaaatatta
    30901 atgctggcta taagcaccgc gataaaacta aggaagctaa cccagccgcc ctggccaatc
    30961 tgcaacagtg ctccgacaac aataatccct gaaatcgcgt tggtgaccga catcaacggt
    31021 gtatgcagcg cgtgcgatac attccacacc acgtaataac cgacaacaca ggccagcgca
    31081 aaaacggtga agtgcccaag aaactctttc ggcgcaacgc ttgccaacca gccgaaaaga
    31141 atgattgcca gcgccatcaa cgcgtattta cgccacggtg agcaggcaca tttttcctcg
    31201 gttttcactt ccggtgccgc tttttgtgcc gcctgcggct gagctgatac ctgaatcggc
    31261 ggtgccggcc aggtaatttc gcccgcacgg atcacggtca cgccgcgaat caccacatca
    31321 tcaaaatcaa cagtgatatt gccgtctttc tctttgcaca acagtttcag cagattaacg
    31381 aggttagtac cgtaaagctg tgaggattgc gtcggcagac ggcccggaag atcggtataa
    31441 ccaatcactt tgacaccatt ttccgtagtg aagatttcac ccggcacggt gtattcacag
    31501 ttgccgccgt tttgggctgc caggtcgaca atcacactac ccgccttcat ggagtcaacc
    31561 atttcacggg taattagctt cggcgctggt ttgcctggaa taagcgcggt ggtgacaatg
    31621 atatcgacct cttttgcctg ggcggtaaag agttccattt ccgctttgat gaacgcgtcc
    31681 gacatcactt tggcatagcc atcgccgctg cccgcttcct ctttaaaatc cagctcgagg
    31741 aattccgcgc ccatactttg aacttgttct ttcacttccg ggcgggtgtc gaatgcacgc
    31801 acaatcgcgc cgagactgtt tgcagcgcca atggcggcca gacccgcaac acccgcacca
    31861 atcaccatca cttttgccgg tggcactttc ccggccgcag taatttgccc ggtaaagaag
    31921 cgcccaaatt catgtgccgc ttcaacaatg gcgcgataac cggcgatatt cgccatcgag
    31981 cttagtgcgt ccagcgattg tgcgcgtgag atacgcggca cagagtccat cgccatcacg
    32041 gtcacgttac gttccgcaag tttttgcatt aattccggat tctgcgcagg ccagataaaa
    32101 ctcaccagcg ttgtcccagg attcagtaac gcaatttcat catctaacgg cgcattgacc
    32161 ttcagaatga tctctgactg ccagacgcta ttcccttcta caatttcagc gcccgcttgc
    32221 acaaacgctt tatcgtcaaa acttgccagt tgacccgcgc cgctctctac cgcgacggta
    32281 aaacccagtt tcagcagctg ttccactgtt tttggcgttg ctgcaacacg ggtttcattg
    32341 gttaaccgtt ctcttggtat gccaattcgc atgatattcc cttccatcgg ttttattgat
    32401 gatggtttgc ctgtgtcagg agccacacaa gccgctcatg tacgagctaa atgttactcc
    32461 gttaaaataa attagtaaca aacgccctat aacgtactga aaattatgcc tgtgatctag
    32521 cgccaaaaat cagtatttcg gcgtgaactc gcaaaatatt aacgattcag ccgtgatagt
    32581 gggataaaca ccttagaacg ccggataaag actgataatt gtcttcggcg ttcgcataaa
    32641 acgagacatc gccccggcac gaatcactac ttaacattaa attaacttat acaattcagt
    32701 tgcttcagta gtattgatgc tgatacggct gttttttaag catagacggc catttgagca
    32761 ggattaaaat tggcttaagg aatgtgatat gaaaaatgac gcagacagtt acaccgttta
    32821 aatgcaataa tcagccacgt ttctcgttaa taacaatacc agtacctggt ttgcgcaagg
    32881 cgaaggatta tttttatgaa gcttaagaac accctcctgg cgtcggcact gctttctgct
    32941 acggcattct ccgttaacgc agcaacagaa ctgacaccgg agcaagcggc agcggttaaa
    33001 ccttttgacc gtgtagtggt taccggtcgt tttaatgcta ttggcgaagc ggtgaaagcc
    33061 gtttctcgtc gcgctgataa agaaggtgcc gcctcttttt atgttgtcga cacttctgat
    33121 tttggtaaca gcggtaactg gcgtgtggtc gctgacctct ataaagccga tgctgaaaaa
    33181 gcagaagaaa caagtaatcg cgtaattaac ggtgttgtcg aactgccgaa agatcaggct
    33241 gttctgattg aaccgtttga cacggtcacc gtccagggct tctatcgtag ccagccagaa
    33301 gtcaatgatg ccatcaccaa agcggcaaaa gcgaaaggtg cctactcttt ctacatcgtt
    33361 cgtcaaatcg atgccaacca gggcggcaac cagcgtatta ctgcattcat ctataaaaaa
    33421 gatgctaaga aacgtatcgt ccagagcccg gatgtgatcc cggcagattc cgaagcagga
    33481 cgtgcagctc tggctgccgg tggcgaagct gcgaagaaag ttgagatccc gggtgttgcg
    33541 actaccgcat caccaagttc tgaagtcggt cgcttctttg aaacccagtc atcaaaaggc
    33601 gggcgttaca ctgtcacgct cccagacggc actaaagtcg aagaactgaa caaagcgacc
    33661 gcagcgatga tggtcccgtt cgacagcatc aaattctctg gcaactacgg caacatgact
    33721 gaagtctcct atcaggttgc gaaacgtgcc gcgaagaaag gtgctaagta ttaccacatc
    33781 acccgccagt ggcaagaacg tggtaataac ctgaccgtca gcgcagatct gtataaataa
    33841 ctattcagtt actcctataa ggcggcttga tgccgccttt cgcattttta gtcacaaatt
    33901 tccccgcgac cattgcatgc agacacatct cttcgtaaaa tcccgcgctt cagtggcaaa
    33961 ctccattttt atgcgttttg ccctaatatt tattcttctt gtcacgtttt aatcactgga
    34021 tatcgatgga aaagaaactg ggactgagcg cactcaccgc gctggtatta agctcaatgc
    34081 tgggcgcggg tgttttcagt ctgccgcaaa atatggcggc agttgccagc ccggcagcac
    34141 tgctcatcgg ctggagtatt actggtgctg gtattttatt actgaccttt gccatgctga
    34201 tcctcacgcg cattcgtccc gaacttgacg gcggtatctt tacctatgcc cgcgaaggat
    34261 ttggtgagct aatcggcttt tgttccgcat ggggatactg gctgtgcgcg gtcatcgcca
    34321 acgtctccta tctggtgatc gttttttccg cgttaagctt ttttacggac acgccggaat
    34381 tgcgcctgtt tggcgatggt aatacctggc aatcgatcgt cggtgcatcg gctttattgt
    34441 ggattgttca ctttttgatc ctgcgcggag tgcaaaccgc tgccagcatt aacctggtgg
    34501 cgacactggc aaaattgttg ccgctgggtc tgtttgttgt gctggcgatg atgatgttca
    34561 aactggatac cttcaagctc gacttcaccg gacttgcact tggcgtaccc gtttgggaac
    34621 aggtgaaaaa caccatgctg atcaccctgt gggttttcat tggtgtggaa ggtgccgtgg
    34681 ttgtttctgc gcgtgcgcgt aataaacgtg atgttggcaa agcgacactg ctggcggttc
    34741 tctccgctct gggcgtttac ttgctggtaa cgctgctttc gctgggcgtg gtggcacgtc
    34801 ctgaactggc tgaaattcgt aacccgtcaa tggccggact gatggtggaa atgatgggac
    34861 cgtggggcga aatcatcatc gctgccggtt tgatcgtttc cgtttgcggt gcgtacttaa
    34921 gctggaccat catggcggca gaagttccgt tcctggcagc cactcataaa gcattcccgc
    34981 gcatttttgc gcgccagaac gcacaagctg cgccatccgc ttcactgtgg ctgaccaata
    35041 tctgcgtgca aatttgtctg gtactcatct ggctgaccgg ttccgattac aacacattgc
    35101 tgaccatcgc ctcagaaatg attctggtgc cctatttcct ggtcggcgcg ttcttgctga
    35161 aaatcgcaac ccgtccactg cataaagcgg taggtgtcgg tgcctgcatt tatggcttat
    35221 ggttattgta cgcctctggt ccaatgcact tgctgttatc cgttgttctg tatgcaccgg
    35281 ggctgctggt tttcctgtac gcgcgtaaaa cgcataccca tgataatgta ctgaatcgtc
    35341 aggaaatggt gctgattggc atgctgttaa ttgcctcagt tccggcgacc tggatgctgg
    35401 tgggataacg ttcatcccat cgttggatag caaaggagat aacgatgggt aaagcccaac
    35461 ccttgccaat attaattact ggcggaggtc gtcgcatcgg cctcgccctc gcctggcatt
    35521 tcattaatca aaagcaaccg gtgattatca gctatcggac gcactatccc gccattgatg
    35581 gactgattaa cgcaggtgcg cagtgtattc aggctgattt ttcgaccaac gacggtgtga
    35641 tggcgtttgc cgatgaagta ctaaaaagca cccatggcct gcgtgctatt ttgcataacg
    35701 ccagcgcgtg gatggcagaa aaaccaggta caccactgac cgacgttctg gcttgcatga
    35761 tgcagatcca cgttaatacc ccatacctac tcaaccatgc actggaaaga ttactgcgtg
    35821 ggcacggaca cgccgccagc gatatcattc actttaccga ttatgtggtg gagcgcggta
    35881 gcgacaaaca tattgcctat gctgcaagca aagcggcact ggataatatg acccgctcgt
    35941 ttgcccgcaa gctggcaccg gaagtgaaag tgaattctat tgcgccatcg ctgatcctgt
    36001 ttaatgagca tgatgatgcc gaataccgac aacaggcgct gaataaatca ctgatgaaaa
    36061 ccgcgcctgg cgagaaagaa gtgatcgacc tggtcgatta cttacttacc agctgctttg
    36121 tcaccggacg cagtttccct cttgatggcg gtcgtcatct gcgttaatgc agctttatcc
    36181 agcatataat caacacccat gcgcttatcc cccagcatgc aacagaacca acaaaagcgg
    36241 cgggcaagcg catcattccg gtgaaatacc acagcgacgc caggtaaaca aaatagggaa
    36301 taatcgacca catgctaaaa atgatggttg cgcgtaaggc ttcaatgccg cgttcgctgg
    36361 caacaatata atgcgcgatg agcgcaaagg tcgggaaaag tggtatcagc ccggcgatat
    36421 aataattttt cgtttttgct aaaacaccaa tcaacagcac taccagcgca ccgagcgcgg
    36481 ctttgattac cagccccatc tttttacctt aacacttcca taacaagtta tcagtagaat
    36541 acctgatgaa accttgttta gaaacgattg atagtaagta aaaacagcgc ggtgtattgt
    36601 gacgttttta tatctaccgt gaatgttatg aacactatcg tatttgtgga agatgatgcg
    36661 gaagtcggtt cactgattgc cgcgtacctg gcaaaacatg atatgcaggt taccgtggaa
    36721 ccgcgcggcg accgggccga agaaactatt ctgcaagaaa atccggattt ggtgttactc
    36781 gacatcatgc taccaggcaa ggacggcatg accatttgtc gtgatttacg cgcaaagtgg
    36841 tccggaccga ttgttcttct aacctctctc gatagcgata tgaaccacat cctggcactg
    36901 gaaatgggtg cctgcgacta tattctcaaa acgacgcccc ctgctgtttt gttagcgcgt
    36961 ttacgtttgc atttgcgtca gaatgagcaa gccacactga gcaaaggtct tcaggaaacg
    37021 tctctgactc cctacaaagc cctgcatttc ggcacgttga ccatcgatcc tatcaaccgc
    37081 gtagtcaccc tggctaacac tgaaatctcg ctctcgacag ctgatttcga attattgtgg
    37141 gaattagcta cccatgctgg gcaaatcatg gaccgcgatg cattgctgaa aaatttacgc
    37201 ggcgtcagtt atgacggact ggatcgtagc gtggacgtgg ctatttcgcg gttaagaaaa
    37261 aaactgctcg ataacgccgc agaaccttat cgcattaaaa ctgttcgtaa caaaggctat
    37321 ctttttgcgc ctcatgcatg ggaataagcg atgaaaaaac tgtttatcca gttttacctg
    37381 ttattgtttg tctgcttcct tgtgatgtct ctgctggttg ggctggtgta caaatttacc
    37441 gccgaacgcg cgggcaaaca gtcgctggat gatttgatga acagttcgct gtatctgatg
    37501 cgcagcgaat tgcgtgagat ccccccacac gattggggta agacgctgaa ggagatggat
    37561 ttaaatctct ctttcgatct gcgagtcgag ccactgagta aataccatct tgatgatatt
    37621 tccatgcacc ggctgcgtgg cggcgaaatt gtcgccctgg acgatcagta cacttttttg
    37681 caacgtatcc cgcgcagcca ctacgtgctg gcagttggtc ctgttcctta tctttattac
    37741 ctccatcaga tgcgattact ggatatcgcc ctgatcgctt ttattgccat ttccctcgcc
    37801 tttccggtgt ttatttggat gcgtccgcac tggcaggata tgttaaaact ggaagcagcg
    37861 gcgcaacgat ttggcgatgg gcatctcaat gaacgtatcc actttgatga aggttcgagc
    37921 tttgaacgac ttggcgtcgc atttaaccag atggcggaca atatcaacgc cttgattgcc
    37981 agcaaaaaac agcttattga cggtatcgct cacgaactgc gaacaccgtt agtgcgcctg
    38041 cgttatcgac tggagatgag cgataacctg agcgccgtcg aatcccaggc gttgaatcgc
    38101 gatatcagtc aacttgaagc tttaattgaa gagctgctga cttatgcccg actcgatcgc
    38161 ccacaaaatg agcttcatct tagcgaacca gacctgccgt tgtggctgtc aacgcatctg
    38221 gcagatattc aggcagtaac gcccgataaa acggtacgga taaaaacgct cgcgcaaggc
    38281 cattatgctg cgttggatat gcgcttaatg gagcgcgtgc tggataattt gctcaataac
    38341 gccctgcgct actgtcactc aacggttgaa accagcctgc tactgtcggg gaatagagcg
    38401 acattaattg ttgaggatga tggcccaggg attgcccctg aaaaccgcga acatatcttt
    38461 gaaccttttg ttcgcctcga tcccagccgg gatcgctcaa ccggcggctg cgggctgggg
    38521 ctggcaattg tccactctat agcactggca atgggcggta cggttaattg tgacaccagc
    38581 gaactgggtg gtgcccgctt ctcgtttagc tggccgttat ggcataacat cccgcaattt
    38641 acctctgctt gacattacgc gcacggtggt caagtcacca ccactgtgct ataaaataag
    38701 gatgttgtaa ctaaagtggt taatattatg gcgcgttacg atctcgtaga ccgactcaac
    38761 actacctttc gccagatgga acaagagctg gctgcatttg ccgctgagct agaacagcac
    38821 aaattgctgg ttgcccgcgt gttctctttg ccggaggtaa aaaaagagga tgagcataat
    38881 ccgcttaatc gtattgaggt aaagcaacat ctcggcaacg acgcgcagtc gctggcgtta
    38941 cgtcatttcc gccatttatt tattcaacaa cagtccgaaa atcgcagcag caagaccgct
    39001 gttcgtctac ctggcgtgtt gtgttaccag gtcgataacc tttcgcaagc agcgttggtc
    39061 agtcatattc agcacatcaa tcaactcaag actacgttcg agcatatcgt cacggttgag
    39121 tcagaactcc ccacagcggc gcgttttgaa tgggtgcatc gtcatttgcc ggggctgatc
    39181 acccttaatg cttaccgaac gctcactgtt ctgcacgatc ccgccacttt acgttttggc
    39241 tgggccaata aacatatcat taagaatttg catcgtgatg aagtcctggc acagctggaa
    39301 aaaagcctga aatcaccgcg cagtgtcgca ccgtggacgc gcgaggagtg gcaaagaaaa
    39361 ctggagagag agtatcagga tattgctacc ctgccacagg acgcgaagtt aaaaatcaaa
    39421 cgtccggtga aggtgcagcc gattgcccgc gtctggtaca agggagatca aaaacaagta
    39481 caacacgctt gccctacacc gcttattgcg ctgattaatc gggataatgg cgcgggcgtg
    39541 ccggacgttg gggagttgtt aaattacgat gccgacaatg tgcaacaccg ttataaacca
    39601 caggcgcagc cgcttcgttt gatcattcca cggctgcacc tgtatgttgc agattaaccc
    39661 ccggctttca tactgccgac catctgttct ggccgtaccc agctgtcaaa ctcggcttcg
    39721 ctaagatacc ccagcgcaag ggccgcagct tttaaggtca gcccttcttt atgcgctttt
    39781 ttggcgatct cggcggcttt gtcataacca atgtgggtgt taagcgcagt caccagcatc
    39841 agcgattcat tgagtaattg attgattcgc tcacgattcg gttcaatgcc cactgcgcag
    39901 tgtttgttaa aactttccat gccatctgcc agcaagcgca ccgattgcag gaaattatgg
    39961 atcaccatcg gacggaagac gttcagttca aagttaccgg aagcgccacc catgttgatc
    40021 gccacatcgt tccccatcac ctgacagcag agcatggtta atgcttcaca ctgcgtcggg
    40081 ttcactttcc ccggcatgat tgagctaccc ggctcatttt ccgggattga gatttcacca
    40141 attccgcagc gcgggccaga ggccagccag cggacatcat tggcaatttt catcagtgac
    40201 gcagccaacc ctttcaacgc gccgtgcgcc tgaaccaggg catcacaggt cgccagcgct
    40261 tcaaatttgt tcggcgcggt aacaaacggt gcacaagtaa tgactgccag ttcatctgcc
    40321 acgcgacgcg catactccgg atgggtattt agtccagtac ccaccgctgt accgccaaga
    40381 gccagttccg ctacatgcgg caggctgtat tcgatatgtt tcagattatg ctcgagcatc
    40441 gctacccagc cggaaatctc ctgccccagc gttaacggcg tggcgtcctg caagtgggta
    40501 cgaccgattt tgacgatatc agcaaatacg cgggattttt cactcagcgt ctgggtcagg
    40561 gttttaagtt gtggaatgag ttgcttgcgc agcgccagta gtgccgcaac gtgcatcgcc
    40621 gtcggaaaga catcgttgga actttggctt ttgttcacgt cgtcgttagg gtgaacttta
    40681 cgttccatcc cgcgcacgcc accgagtaat tcactggccc ggttagccag cacttcgttc
    40741 atgttcatgt tactttgcgt gccggagccg gtctgccaga tagccagcgg gaattcgtcg
    40801 tcatgctgtc ctgccagtac ttcatccgcc gcctgccgaa tggcgctcgc tttctcttca
    40861 gacaacaagc ctaaatcttc attaactttt gccgctgcgc gcttggttag cgccagcgca
    40921 tgaatcagtg aggtgggcat tttctccgtc gaaatgcgga aatgctccag cgagcgctga
    40981 gtttgtgcgc cccacagctt atctgccggg acatcaatcg ctcccatcga atctttttcg
    41041 ctgcgtactg tattcatggc ctgctcctca cctgattaat tttttctttc tgttttgctt
    41101 tcgttaagca acttttagct tactcattat ttaccatttg ataacaaatg tttggtcttt
    41161 cgtgccatgt aaaaaaaccg ccccgaaggg cggctctgtt tatttcacac agcgggtgca
    41221 ctgtgtgagt tgtatctgct ggaagaagtc atttccttta tcatccacaa ggataaatgc
    41281 cgggaaatct tccacttcaa ttttccagat ggcttccatt cccagttccg gatattcaac
    41341 acattccagg ctcttaatgc ttccctgcgc caatacagct gccggaccac cgatactgcc
    41401 aaggtagaag ccgccgtgtt ttttacaggc atccgtcacc tgctggctgc ggttgccttt
    41461 cgccagcatg atcatacttc cgccctgcgc ttgcagttga tcgacataag aatccatccg
    41521 tccggcggtc gttgggccaa gagaaccgga ggcataacct tccggcgttt tggccggacc
    41581 cgcgtagtaa atcggatgat ctttaatata ctgcggcagc ccttcgccgt tatccatccg
    41641 ctctttcagt ttggcgtgag caatatcacg accaacgata atcgtgccat tcagcgataa
    41701 gcgtgtagaa acgggatact gcgacaactg tgcgaggatc tctttcatcg gacggttaag
    41761 gtcaacgcgc accgcttcgc cttctcccgc tttacgcagc tcttccggga tatatttgcc
    41821 tggattatgt tccagttttt cgatccagat cccctgacgg ttgatcttcg ctttgatatt
    41881 acggtcagca gagcaggaga cgcccatacc gaccgggcag gatgcgccgt gacgtggcag
    41941 gcgaatcacg cggatgtcgt gagcgaagta tttaccacca aactgcgcac ccagaccaag
    42001 attttgcgct tcgatcagca attctttttc cagttccaca tcgcggaacg cctgaccgtg
    42061 ctcattccct tccgttggca gttcgtcata gtatttagcg gaagccagtt tcaccgtttt
    42121 aaggttcgtt tccgcagaag ttccaccaat tacgaacgca atatgatacg gaggacaggc
    42181 cgccgtaccc agcgtgcgca tcttctcaac caggtaattt ttcagtttcc ccggcgttag
    42241 taacgctttg gtttcctgat agagatacgt cttgtttgcc gaaccaccac ctttggcgat
    42301 acagaggaat ttgtactcgt cgccatcaac ggcataaaga tcgatctgcg ctggtagatt
    42361 ggtgccggta ttcacctctt tatacatatc cagcggcgcg ttttgcgagt agcgcagatt
    42421 atcttcgata taagtgttat agacaccgcg tgccagcgcc gcttcatcac cgccaccggt
    42481 ccatacacgc tgcccttttt tgccaacaat aatcgcggtg ccggtatcct gacaggttgg
    42541 cagaacgcct ttcgccgcga tgtcggagtt gcgcaggaat tgcagcgcca catatttatc
    42601 attttcgctg gcttccgggt cacgcagaat gtcggccacc tgttgttggt gagccggacg
    42661 cagcataaat gacgcatcat gaaacgcctg acgcgccaac aaagttaacg cttcgggtgc
    42721 gactttcaaa atctcctgcc cttcaaattc agatacgcta acgtgttcgc tggttagcag
    42781 gtaatactca gtatcatctt ttttgagtgg aaaaggagcc tgataatgaa agggtttgtt
    42841 tgacattgtt ctctcactta ctgcctggtt tggttatgct ctgggcgggt gttccgttgc
    42901 cctgttaaaa gcgagtaaca atatcctaca cactttttta acaaaaactg agactagtac
    42961 gactttttgc ggctccaggt tacttcccgt aggattcttg ctttaatagc gggattaatt
    43021 tccacattaa aacagggatt gatcatgcaa aaactcatta actcagtgca aaactatgcc
    43081 tggggcagca aaacggcgtt gactgaactt tatggtatgg aaaatccgtc cagccagccg
    43141 atggccgagc tgtggatggg cgcacatcca aaaagcagtt cacgagtgca gaatgccgcc
    43201 ggagatatcg tttcactgcg tgatgtgatt gagagtaata aatcgactct gctcggagag
    43261 gccgttgcca aacgctttgg cgaactgcct ttcctgttca aagtattatg cgcagcacag
    43321 ccgctctcca ttcaggttca tccaaacaaa cgcaattctg aaatcggttt tgccaaagaa
    43381 aatgccgcag gtattccgat ggatgccgcc gagcgtaact ataaagatcc taaccacaag
    43441 ccggagctgg tttttgcgct gacgcctttc cttgcgatga acgcgtttcg tgaattttcc
    43501 gagattgtct ccctgctcca gccagtcgca ggtgcacatc cggcgattgc tcacttttta
    43561 caacagcctg atgccgaacg tttaagcgaa ctgttcgcca gcctgttgaa tatgcagggt
    43621 gaagaaaaat cccgcgcgct ggcgatttta aaatcggccc tcgacagcca gcagggtgaa
    43681 ccatggcaaa cgattcgttt aatttctgaa tgttacccgg aagacagcgg cctgttctcc
    43741 ccgctgttgc tgaatgtggt gaaattgaat cctggcgaag cgatgttcct gttcgctgaa
    43801 acaccgcacg cttacctgca aggcgtggcg ctggaagtga tggcgaactc cgataacgtg
    43861 ctgcgtgcag gtctgacgcc taaatacatt gatattccgg aactggttgc caacgtgaag
    43921 ttcgaagcca aaccggctaa ccagttgttg acccagccgg taaaacaagg tgcagaactg
    43981 gacttcccga ttcctgtgga tgattttgcc ttctcgttgc atgaccttag tgataaagaa
    44041 accaccatca gccagcagag tgccgccatt ttgttctgcg tcgaaggcga tgcaacgctg
    44101 tgtaaaggtt ctcagcagtt acagcttaaa ccgggtgaat cagcgtttat tgccgccaac
    44161 gaatcaccgg tgactgtcag aggccacggc cgtttagcgc gtgtttacaa caagctgtaa
    44221 gagcttactg aaaaaattaa catctcttgc taagcttatt aaaggcttat aacaccttca
    44281 ggcggccagt ccgcctgatt tcattttatg gataatcatt atgaataaat cgctggtagc
    44341 ggtaggcgtc attgttgcgc taggcgtagt ctggacaggc ggcgcatggt atacaggcaa
    44401 gaagattgaa acccatctcg aagacatggt cgcgcaggcg aacgcgcaac tcaaactgac
    44461 cgctcctgaa tccaacctgg aagtgagtta tcaaaactat catcgcggcg tattcagcag
    44521 tcagctgcaa ctgttggtga aacccattgc cggaaaagta aatccgtgga ttaaaagcgg
    44581 tcagagcgtc atcttcaacg aatcggttga tcatggtccc ttcccgcttg cccagcttaa
    44641 aaaactgaat ctgatcccgt cgatggcatc aattcaaacc acgctggtta ataacgaagt
    44701 aagcaaacca ctgtttgata tggcaaaagg tgaaacgcct tttgagatta actcccgcat
    44761 tggttacagc ggagattcca gttccgacat ttcgctcaag ccactgaatt acgagcaaaa
    44821 ggatgaaaaa gtcgccttta gcggcggcga gttccagtta aatgcagaca gagacggcaa
    44881 agctatctcc ctttccgggg aggcgcaaag tggtcggata gacgctgtta acgaatacaa
    44941 ccagaaagta cagttgacct ttaataatct gaaaaccgac ggttccagca cgctggcaag
    45001 ttttggtgag cgcgtaggaa accaaaaact gtcactggaa aaaatgacca tttcagtgga
    45061 aggcaaagaa ctggcactgc tggaaggcat ggagatcagc ggtaaatcgg atctggtcaa
    45121 tgatggtaaa acgatcaata gccaactgga ttactcgcta aacagcctga aggtacagaa
    45181 tcaggatctg ggcagcggca agctgacttt aaaagtcggc caaattgatg gcgaagcctg
    45241 gcatcagttt agccagcaat ataacgcgca aactcaggcg ctgctggcac agccagaaat
    45301 tgccaacaat cccgaacttt atcaggaaaa agtgacggaa gccttcttta gcgccctgcc
    45361 gctgatgttg aaaggcgatc cggtgattac tatcgcgccg ctaagctgga aaaacagtca
    45421 gggtgaaagt gcgctgaatc tgtcgctgtt cctgaaagat ccggcaacga ctaaagaagc
    45481 gccgcaaacg ctggcgcagg aagtagaccg ttcggttaaa tctctggatg cgaaactgac
    45541 cattccggtg gatatggcaa ctgagttgat gactcaggta gcgaagctgg aaggttatca
    45601 ggaagatcaa gcgaaaaaac tggcgaaaca gcaagttgaa ggtgcatcag caatggggca
    45661 gatgttccgt ctgaccacct tgcaggacaa taccatcacc accagcctgc aatatgctaa
    45721 cggtcagatc acgttaaacg ggcagaaaat gccactggaa gattttgttg ggatgtttgc
    45781 gatgccggca ttaaacgttc cggctgtacc cgctattccg cagcagtaat tcacagccaa
    45841 aatgccggag atttttctct ccggcatttt ttcttacttc agcataaagt catactgaat
    45901 actcattttc acatcccagt aattcaggcg atcgcccagc gcactgccat cgcgcttttc
    45961 ccgcccttta tcggcagcaa gaaagaaatt catcccttgt aacggccctt cgctgacccg
    46021 atagttcata ccacctaaga tacccgcgcc ttcaatccgc acatctcgtt tactggattc
    46081 atagccgcga gtcacaatcg gtgcaaatgt cagcgtaaat tgcggcgtta aacgatagtt
    46141 gacacccaat tgccaggact gcattccttc acggttacga tcaagactta atgaaaaggg
    46201 atagccaatg tcggtatcca ccacggcacc attcattgca tggcgcaaat aaccaccact
    46261 gccaaatacg cttccccacg gcgcattcca ggtcaattgt ccactaacca acgccgtttc
    46321 attgggctgg ctggtattac gacttaaacc ttcaagttgc gcgtaaaagc cgagcaactc
    46381 ccctttcaac gtataattcg ggtcaagcgg tatgcttttc tcgagaatac tttgtatctg
    46441 attggaaaca tctttctgta ccgatgcccc cagagaatag cgactggtat catcatgcca
    46501 actggcggct aaaaaagaac gtgcgcgatg tttatcgtat tttcccgtct ggctgttata
    46561 caccccttca tcacgaatac cggttgttga atcggtccat aaaaatactc gtgaccgcca
    46621 cattccccac agatcaatat ttggcgttaa agcccagtaa cctaatgctc cttcagaaac
    46681 tgtcggtaat gtacgttgcg aactgccagc cattaacgga atacgataag gtaagctacc
    46741 ataactataa tccgtcccga agcgacctaa ttttagcagg ctattttcac tgggttttat
    46801 ttttactgcc cccagggcaa agccaaaact tttatcacca tcaagatacc accgggtact
    46861 catatcagca cgagcaccta atttataaac ataatacgcc ccgccttcaa cgccaataat
    46921 attggaataa taaccactat tgaaatcgag caatcctccc tgtacccatg cgtaaatatc
    46981 tcttcccgtg ccagcactgg gcttatcagc cctgcgtaat tcgtttttca gtctgatgcg
    47041 tagtcccgct tcatcgtcag ccggttgcgc cgcgtaagca gaggtaaggc cagaggcagc
    47101 cgtcaggcaa ataacggcca tggccactat ttttctcata gttgcacctt tgatgttctg
    47161 atta
//
LOCUS       DAZGQL010000026        47155 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.026, whole genome shotgun sequence.
ACCESSION   DAZGQL010000026 DAZGQL010000000
VERSION     DAZGQL010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 47155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47155
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.026"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            146..688
                     /gene="zapC"
                     /locus_tag="ACHKIR_001728"
     CDS             146..688
                     /gene="zapC"
                     /locus_tag="ACHKIR_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HFO9989478.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFNLEQAV"
     gene            complement(685..1794)
                     /gene="ycbX"
                     /locus_tag="ACHKIR_001729"
     CDS             complement(685..1794)
                     /gene="ycbX"
                     /locus_tag="ACHKIR_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HFO9989479.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEVLATAPAKIYGAAA
                     ADDTVNITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLTR"
     gene            2038..4146
                     /gene="rlmKL"
                     /locus_tag="ACHKIR_001730"
     CDS             2038..4146
                     /gene="rlmKL"
                     /locus_tag="ACHKIR_001730"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HFO9989480.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPVMAA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            4158..6065
                     /gene="uup"
                     /locus_tag="ACHKIR_001731"
     CDS             4158..6065
                     /gene="uup"
                     /locus_tag="ACHKIR_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO9989481.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKIEEAAAPKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            6195..7448
                     /gene="pqiA"
                     /locus_tag="ACHKIR_001732"
     CDS             6195..7448
                     /gene="pqiA"
                     /locus_tag="ACHKIR_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HFO9989482.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADDPVCPRCGTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            7453..9093
                     /gene="pqiB"
                     /locus_tag="ACHKIR_001733"
     CDS             7453..9093
                     /gene="pqiB"
                     /locus_tag="ACHKIR_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HFO9989483.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            9090..9653
                     /gene="pqiC"
                     /locus_tag="ACHKIR_001734"
     CDS             9090..9653
                     /gene="pqiC"
                     /locus_tag="ACHKIR_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HFO9989484.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVAVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLASVWSQEAASIAQEIKRLP"
     gene            9909..10076
                     /gene="rmf"
                     /locus_tag="ACHKIR_001735"
     CDS             9909..10076
                     /gene="rmf"
                     /locus_tag="ACHKIR_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0141014 - ribosome hibernation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HFO9989485.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(10146..10664)
                     /gene="fabA"
                     /locus_tag="ACHKIR_001736"
     CDS             complement(10146..10664)
                     /gene="fabA"
                     /locus_tag="ACHKIR_001736"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008693 -
                     (3R)-3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HFO9989486.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(10733..12493)
                     /gene="ycbZ"
                     /locus_tag="ACHKIR_001737"
     CDS             complement(10733..12493)
                     /gene="ycbZ"
                     /locus_tag="ACHKIR_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO9989487.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARALQSDAGQLVGGHYEVSGHTIRLR
                     NAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTLTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANIR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            12679..13131
                     /gene="matP"
                     /locus_tag="ACHKIR_001738"
     CDS             12679..13131
                     /gene="matP"
                     /locus_tag="ACHKIR_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HFO9989488.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(13207..14259)
                     /gene="ompA"
                     /locus_tag="ACHKIR_001739"
     CDS             complement(13207..14259)
                     /gene="ompA"
                     /locus_tag="ACHKIR_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HFO9989489.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDVYTRLGGMVWRADTKSNVPGGASTKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKH
                     FTLKSDVLFTFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQA
                     LSERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRV
                     EIEVKGIKDVVTQPQA"
     gene            complement(14615..15124)
                     /gene="sulA"
                     /locus_tag="ACHKIR_001740"
     CDS             complement(14615..15124)
                     /gene="sulA"
                     /locus_tag="ACHKIR_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS-induced cell division inhibitor SulA"
                     /protein_id="HFO9989490.1"
                     /translation="MYTSGYAHRASSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            15342..15971
                     /gene="sxy"
                     /locus_tag="ACHKIR_001741"
     CDS             15342..15971
                     /gene="sxy"
                     /locus_tag="ACHKIR_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HFO9989491.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(15934..18096)
                     /gene="yccS"
                     /locus_tag="ACHKIR_001742"
     CDS             complement(15934..18096)
                     /gene="yccS"
                     /locus_tag="ACHKIR_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HFO9989492.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWKFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNQALAGLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(18106..18552)
                     /gene="yccF"
                     /locus_tag="ACHKIR_001743"
     CDS             complement(18106..18552)
                     /gene="yccF"
                     /locus_tag="ACHKIR_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFO9989493.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            18675..20729
                     /gene="helD"
                     /locus_tag="ACHKIR_001744"
     CDS             18675..20729
                     /gene="helD"
                     /locus_tag="ACHKIR_001744"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HFO9989494.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPGEKVVRLHGTEWGETQRFYHHLDAHWQRWSSEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEVIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESHLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPSVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(20761..21219)
                     /gene="mgsA"
                     /locus_tag="ACHKIR_001745"
     CDS             complement(20761..21219)
                     /gene="mgsA"
                     /locus_tag="ACHKIR_001745"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFO9989495.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(21315..21977)
                     /gene="yccT"
                     /locus_tag="ACHKIR_001746"
     CDS             complement(21315..21977)
                     /gene="yccT"
                     /locus_tag="ACHKIR_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HFO9989496.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     HGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            22150..22563
                     /gene="yccU"
                     /locus_tag="ACHKIR_001747"
     CDS             22150..22563
                     /gene="yccU"
                     /locus_tag="ACHKIR_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFO9989497.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLAEVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(22608..22925)
                     /gene="hspQ"
                     /locus_tag="ACHKIR_001748"
     CDS             complement(22608..22925)
                     /gene="hspQ"
                     /locus_tag="ACHKIR_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HFO9989498.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(22983..24173)
                     /gene="rlmI"
                     /locus_tag="ACHKIR_001749"
     CDS             complement(22983..24173)
                     /gene="rlmI"
                     /locus_tag="ACHKIR_001749"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HFO9989499.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPNESIDIAFFTRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGPITGELPPALLPIEEHGMKLLVNIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            24268..24546
                     /gene="yccX"
                     /locus_tag="ACHKIR_001750"
     CDS             24268..24546
                     /gene="yccX"
                     /locus_tag="ACHKIR_001750"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFO9989500.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKKLGLAGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIH"
     gene            complement(24543..24872)
                     /gene="tusE"
                     /locus_tag="ACHKIR_001751"
     CDS             complement(24543..24872)
                     /gene="tusE"
                     /locus_tag="ACHKIR_001751"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HFO9989501.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGITLSPEHW
                     EVVRFVRNFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(24963..25622)
                     /gene="yccA"
                     /locus_tag="ACHKIR_001752"
     CDS             complement(24963..25622)
                     /gene="yccA"
                     /locus_tag="ACHKIR_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HFO9989502.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(26032..27051)
                     /locus_tag="ACHKIR_001753"
     CDS             complement(26032..27051)
                     /locus_tag="ACHKIR_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO9989503.1"
                     /translation="MGRPRKNKKDNVLPPRVRSNGYSYVWKPEGSTRTIGLGRVRKTS
                     VAKVWQNYELEKAKLHNIMTVAKLWHMFMDSPAFTELAPRTQKDYRQHQKALLMVFGK
                     VLADNVKTEQVRIFMDKRGLESKTQANHELASLSRVYGWGYERGYVKNNPCKGVRKFS
                     LKARTVYITDEQYAAIYAEAIPQLRIAMEISYLCAARLGDVLELKWQDIMDKGIYIEQ
                     NKTGTKQIKEWSPRLRTAIQLARNVSSCTCEYVINTTKGGKVIAKTLNNWWNQAKRSA
                     EQKVGVPFGCNFHDIKAKGISDYEGSSRDKQIFSGHKTENQVLIYDRKTKITPTLDLP
                     LVVSK"
     gene            complement(27029..27271)
                     /locus_tag="ACHKIR_001754"
     CDS             complement(27029..27271)
                     /locus_tag="ACHKIR_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HFO9989504.1"
                     /translation="MYELTLSPAEIKEITKYERYTKQQHQLRLHGIPFVIGPKNEPIV
                     LRRDIPHGLTTMPKTSELVSAEPDFEALNNGKTKKK"
     gene            complement(27339..29777)
                     /locus_tag="ACHKIR_001755"
     CDS             complement(27339..29777)
                     /locus_tag="ACHKIR_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFO9989505.1"
                     /translation="MSTDKEEFALYCEAKNDKVRKRFGIKGGFYWSTEKKLSVAISRC
                     VVAMDEAGYDADDFKKPVRVHFPVVNDLPPEGVFDTEFCNRYEKGGEDGITMIFIAPS
                     PSVQEKPASTDNTNVNGEDMAEIEDNMLLPISGQELPIRWLAQHGSEKPVTHVSREEL
                     QALHIARAEELPAVTALAISHNTKLLDPLEIRDLHKLVRDTDKVFPNPVNSSLGLMTA
                     FFEAYLDADYTDRGLLTKEWMKGNRVSRITRTASGANAGGGNKTDRNPDLVHTLDTLD
                     VEAAAATLPMDFNIYEIPVSVYRRAKEIVLKKESPFREWSAALRATPGILDYSRAAIF
                     ALIRSAHPEFYHYPGRLQGYINANLTETDHENPTAETLMAARHTPEKDILEEINDGLA
                     AECKTEEEKNDEENPQPSGAMADEQATAETMAADTVEHHQDPQPLDDKSQVKVTAEEV
                     NKIMEAADISRPDADKLLAASRGEFVEGISDPNDSKWVKGIETCDSVNQNQLETEQND
                     QKAEQNSQNALQDEPETKQSEPVAQQEEEKVCTACGQSGGGNCPDCGAVMGDATYQET
                     FDEKNQAEVQEDDPEEIEGAEHPNNENAGNDQHHTSDSETGEAADPLIAVNGHHEITS
                     TSRTCDHLMIDLETMGKNPNAPIISIGAIFFDPQTGDMGPEFSKTIDLETAGGVIDRD
                     TIKWWLKQSREAQSAIMTDEIPLDDALLQLREFIDENSGEFFVQVWGNGANFDNTILR
                     RSYERQGIPCPWRYYNDRDVRTIVELGKAIDFDARTAIPFEGERHNALDDARYQAKYV
                     SAIWQKLIPSQADF"
     gene            complement(29870..30058)
                     /locus_tag="ACHKIR_001756"
     CDS             complement(29870..30058)
                     /locus_tag="ACHKIR_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFO9989506.1"
                     /translation="MTSAFALVMTVFLITGEPQNVITGIYASKESCLRARDEQKISGE
                     CLPLKKVSLYLNNEIPAG"
     gene            complement(30055..30243)
                     /gene="dicB"
                     /locus_tag="ACHKIR_001757"
     CDS             complement(30055..30243)
                     /gene="dicB"
                     /locus_tag="ACHKIR_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFO9989507.1"
                     /translation="METLLPNVNTSEGCFDIGVLLSNREFTEDAIKMRKYEPYLLNDN
                     SILSRIALLKLGIFGGQQ"
     gene            complement(30729..30881)
                     /locus_tag="ACHKIR_001758"
     CDS             complement(30729..30881)
                     /locus_tag="ACHKIR_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFO9989508.1"
                     /translation="MDTLNLGNNESLVCGVFPNQDGTFTAMTYTKSKKFKTEAGARRW
                     LARNTN"
     gene            31156..31458
                     /locus_tag="ACHKIR_001759"
     CDS             31156..31458
                     /locus_tag="ACHKIR_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004142601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HFO9989509.1"
                     /translation="MKIISVKTLRDFWAENPDAEQPLRAWVDEAAKADWKSPADIKAQ
                     YRTASILKNRRVVFNVKGNHYRLIVAIAYQRGWVFVKFIGNHKEYDAIDADTIELE"
     gene            31461..31820
                     /locus_tag="ACHKIR_001760"
     CDS             31461..31820
                     /locus_tag="ACHKIR_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007374288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO9989510.1"
                     /translation="MNIKPIRTEQDYEAALRAVEPMFDNEPEIDTPEGDFFEVMCLLI
                     EEYEKKHYPIEPPSPIEAIRFRMEQQGLTVKDLEPAIGKKNRVYEVLNGTRSLTLPMI
                     RRLHNQFGIPLESLVGL"
     gene            complement(31867..32259)
                     /locus_tag="ACHKIR_001761"
     CDS             complement(31867..32259)
                     /locus_tag="ACHKIR_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000578360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO9989511.1"
                     /translation="MIFVKRLQQVLQELNINQSELGRRLGVKPQSVQGWLKGVMPRMD
                     KLEKLAELSQHPVHWFFMEEETLGDKMAVSSNDNQPQLTEQQRKIISLLDELPQSDAE
                     QIIRDMEQKRDFYKRKLEELLRQKNKTA"
     gene            32386..32646
                     /locus_tag="ACHKIR_001762"
     CDS             32386..32646
                     /locus_tag="ACHKIR_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO9989512.1"
                     /translation="MQKSTQVKILSIMSQSELGRRLGKTPQTISGWFKKRVPAEEVIP
                     ACEALDWGVTPHELRPDKYPNPTDGLPVEYQANAQAAAGVDS"
     gene            32643..33080
                     /locus_tag="ACHKIR_001763"
     CDS             32643..33080
                     /locus_tag="ACHKIR_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000693932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO9989513.1"
                     /translation="MKIKHEHIRMAMNAWLLYPRVGRKKIADDIATAYFELEMTYPPM
                     HDTSTTEGIGLNIQNIFRWLEKDTPDAVEKIQALIPAILTVLPRELRYHLSIFDTVER
                     RALLAAQEALSTAIDAHDDAVQAVYRKAYFSDGGSSGESVVVH"
     gene            33167..34177
                     /locus_tag="ACHKIR_001764"
     CDS             33167..34177
                     /locus_tag="ACHKIR_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1376 domain-containing protein"
                     /protein_id="HFO9989514.1"
                     /translation="MAALPYMQLYIADYLADTMHLSAEEHGAYLLLMFNYWQTGKPIP
                     KNRLAKISRLTNERWADVEPSLREFFCDNGDEWVHLRIEEDLASVREKLTKKSAAGKA
                     SVQARRSRKEVYVQTKQERDLTGVQTNVGVVFEHDANTKATNKDTDLKELNPTHNVRE
                     RESIPTSELHGAPLQTAEPEYLDGLSEPIGKFSMTTVWQPSPDFRQRAAVWGMALPEP
                     EFTPAELAAFRDYWMAEGKVFTQVQWEQKFARHVQHVRAQVKPVSKGVSHAASGGTAS
                     RAIQEIRAAREQWERDNGFISNGNGLEAVGAYGGGVFEPLDSEERGRTFEALDCPDWR
                     DD"
     gene            34170..34631
                     /locus_tag="ACHKIR_001765"
     CDS             34170..34631
                     /locus_tag="ACHKIR_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFO9989515.1"
                     /translation="MTEQQIRLVCRQCMDRCRAGETWPPDLAEFVALISGSGANPFGL
                     TVDAVMEEYRRWRNESWRYDGSDKYPWPQPVLYHICLEMRTRGIERQMTQGELKRLAE
                     RQLTKWAKHVGNGMSVPPVRRQLEGAKHPQGPTPIERLKQEYERRKAAGFI"
     gene            34665..35381
                     /locus_tag="ACHKIR_001766"
     CDS             34665..35381
                     /locus_tag="ACHKIR_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFO9989516.1"
                     /translation="METVFDALKALKKASSQVVASRLGISREDAVNELWKLKRRGEAD
                     NKGSMWWLIQAGESEPVSPVPKVTAQMLIKAIEQHGPQTADELALMFGITSRRANSSL
                     AMAISKGRLIRVNQGGKFRYCIPVADLPAEPEAASVAETDGKAFPQPAGVALPVREAE
                     TQEDIKTETVADIVQSLPSFTETRADDLVLPSLHVANRELRRAKGQVQKWERVCAALR
                     ELNKHRDILRDITATRELLR"
     gene            35414..35695
                     /locus_tag="ACHKIR_001767"
     CDS             35414..35695
                     /locus_tag="ACHKIR_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO9989517.1"
                     /translation="MILRQCAGTMTVESIGSLIGRSGAAVRTKARELGICMMLRGDYH
                     QSAKCSQRDIELARQLHQRGVPRREIAEKFGMKLGAVNNYVYFDRRVQA"
     gene            35692..35997
                     /locus_tag="ACHKIR_001768"
     CDS             35692..35997
                     /locus_tag="ACHKIR_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001376361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HFO9989518.1"
                     /translation="MRVRIYIAGPMTGYENFNREAFHKAEEALKQEGHTVLNPAVLPD
                     GLTQPHYMDICMAMIRCVDAVYMLKGWQRSAGAKAELALAEKLGHAVIFQEATSEKN"
     gene            35984..36586
                     /locus_tag="ACHKIR_001769"
     CDS             35984..36586
                     /locus_tag="ACHKIR_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFO9989519.1"
                     /translation="MRKINYQTLREIAKLATQGEWVAFISPGTDTYAVHTPGDERCGD
                     IIKWPGFDDQKNAENNAEFIAAFNPEVVQALLDEREAQSKRIAELEANLVALAAENAL
                     ARKAVQAFCDVVGDNTEVICEEVGRDGVLVILEAMKATGNMPATDAFLADVRAEVFND
                     LCAAFVRHAKVAGLDDADTVTFKEATEALLHCAEQLRAPE"
     gene            36682..36864
                     /locus_tag="ACHKIR_001770"
     CDS             36682..36864
                     /locus_tag="ACHKIR_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9989520.1"
                     /translation="MRKFKIIIETGIAGGDFEDVFEVDDDATPDEIHDEAKEIFFNYC
                     NYSYHEIKDEEEEQNG"
     gene            36857..37042
                     /locus_tag="ACHKIR_001771"
     CDS             36857..37042
                     /locus_tag="ACHKIR_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9989521.1"
                     /translation="MADFGSTKYNASFEEWHELLMDYAELRGGSAADAEAWRDDYEAG
                     KTPVEAYCDEWGEDDED"
     gene            37148..37351
                     /locus_tag="ACHKIR_001772"
     CDS             37148..37351
                     /locus_tag="ACHKIR_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019705292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9989522.1"
                     /translation="MTIVKTHTGIVITKDGPQVKKLHQTERMWVVDKNEFYHKETGRR
                     HFAENTRRRLLIDTIKPVEVKHV"
     gene            37344..37583
                     /locus_tag="ACHKIR_001773"
     CDS             37344..37583
                     /locus_tag="ACHKIR_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9989523.1"
                     /translation="MFKQNEKSIAQIAEYIPRACRGMQLQEAKARLEKKIALYINDGC
                     DAAVLNAAFAPALNSHTRESFFSCIAAQIHKGGNQ"
     gene            37580..37924
                     /locus_tag="ACHKIR_001774"
     CDS             37580..37924
                     /locus_tag="ACHKIR_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFO9989524.1"
                     /translation="MSKIDYQALREAAQNYRSMLAWYQEKPDSPNAEQDCDAALSAFK
                     REIRHREVDIIADLLDELEEAKQRIDEQESRTVKLPEPFKLAKSSSGLTYYYADEVNA
                     ALTAAGIRIEGE"
     gene            37926..38369
                     /locus_tag="ACHKIR_001775"
     CDS             37926..38369
                     /locus_tag="ACHKIR_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9989525.1"
                     /translation="MTTITKERLQWLANISGRDDIDDIDGGEIRELALIALASLEAEP
                     VVYMWDSERKDIDAPGYYRTEHLVFAESSVKQWGGRVVPLYTAPPAPIVPAVLERLRA
                     IVADPRALPRRKEWVSGQQYSYVLLENVEAMVDEACRAAMLQGDK"
     gene            38369..38512
                     /locus_tag="ACHKIR_001776"
                     /pseudo
     CDS             38369..38512
                     /locus_tag="ACHKIR_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311037.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            38540..39070
                     /locus_tag="ACHKIR_001777"
     CDS             38540..39070
                     /locus_tag="ACHKIR_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9989526.1"
                     /translation="MEEAKELHEDLVRSHISKALSGEKMKKKDLDADLCWIHGVIVQA
                     SWFVKASLEQNALTGNSQVIPDDGREQFETLIRFHADDKNHETLLLRANEGMNYRDPN
                     VDLAWIFWKSSREHILPGKSDNNPALGDQVSELTMLVKRLASSLKSVNKSSKLPDKAM
                     DYLKRNGLISAEDILR"
     gene            39368..39523
                     /gene="hokD"
                     /locus_tag="ACHKIR_001778"
     CDS             39368..39523
                     /gene="hokD"
                     /locus_tag="ACHKIR_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO9989527.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYESEK"
     gene            complement(39630..39983)
                     /locus_tag="ACHKIR_001779"
     CDS             complement(39630..39983)
                     /locus_tag="ACHKIR_001779"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9989528.1"
                     /translation="MNKTICFLLVFAISGCVNPYNYREEQNVIISFKTNSPPQEIQEC
                     ILSEWQRTPLLATITSQKIGKYYSVLAVGDNADIYKLADGSTIIDFYSLRGVLDPTNG
                     KSKRIQGIKSCISTK"
     gene            complement(40045..40344)
                     /locus_tag="ACHKIR_001780"
     CDS             complement(40045..40344)
                     /locus_tag="ACHKIR_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO9989529.1"
                     /translation="MTYEVIITSEAEQQIINLHRYITEKAGSAIADNYANALLDYLDG
                     FSTFPHRGNKRDDIRQGMRVTHFRHRTIIAFAVDGNKVFIAGVYHGGQSYESDFL"
     gene            complement(40350..40607)
                     /locus_tag="ACHKIR_001781"
     CDS             complement(40350..40607)
                     /locus_tag="ACHKIR_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HFO9989530.1"
                     /translation="MRTTQQFSITLTNEMADMVRARVASGAYASESEVIREGLRALNE
                     RDKAIEAWLTHSAAPSLDSIRENPNNGRSISQVRAAIRAGK"
     gene            40743..41021
                     /locus_tag="ACHKIR_001782"
     CDS             40743..41021
                     /locus_tag="ACHKIR_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9989531.1"
                     /translation="MAHDTRFYNSDNSAAPASRHGRRSHAFKSDWLQHPPCTEEQAEW
                     LIQCYRRRGYEFEKALSFDRRHWIISVRLPYSERPPRPSRTFQQRIWR"
     gene            41023..42072
                     /locus_tag="ACHKIR_001783"
     CDS             41023..42072
                     /locus_tag="ACHKIR_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO9989532.1"
                     /translation="MRVLLRPVLVPELGMVLLKPGRESMSAFHNGRILVEPEPKNMRG
                     LPSGVVPAVRQPLAEDKTLLPFFSNERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLDQLAQQNLAAWMIDVIRHAMNDTQERE
                     LSLAELCWWAVCHQVADALPESVLRRSLGLPAEKIRSVYRESDIVPGEQTAISILKQR
                     TKNIALPLHVHQQQNPPQKKTVVSIAVDPESPESFMRRPKRRRWVNEKYTRWVKTQPC
                     ACCGKPADDPHHLIGHGQGGMGTKAHDIFTLPLCREHHNELHADPLKFEKKYGSQIEL
                     IFRFLDHAFATGVLG"
     gene            42085..42459
                     /locus_tag="ACHKIR_001784"
     CDS             42085..42459
                     /locus_tag="ACHKIR_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO9989533.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFVSKSGERYRRAVALIVRQQ
                     QLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMLEGQVKK"
     gene            42456..43277
                     /locus_tag="ACHKIR_001785"
     CDS             42456..43277
                     /locus_tag="ACHKIR_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFO9989534.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRERATELLTEYALSRCDRVAALRKLPAEIKPAVMRIMASY
                     AFEDYARSAASKKQCPCCHGKKFIESEVFTNKIQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESEKQGVPVFRNCQRCGGRGY
                     ERLPSTEAFNAICNVTDAISLDTWKKTVKRFYDTLVVQFDIEEAWAEQQLKKVTR"
     gene            43505..43702
                     /locus_tag="ACHKIR_001786"
     CDS             43505..43702
                     /locus_tag="ACHKIR_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFO9989535.1"
                     /translation="MLKQQDMTETARVVFNELSVTEPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQFGCYRRLQS"
     gene            43853..44905
                     /locus_tag="ACHKIR_001787"
     CDS             43853..44905
                     /locus_tag="ACHKIR_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001575625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFO9989536.1"
                     /translation="MLSTVKISSCELINADCLEFIRSLPENSVDLIVTDPPYFKVKPE
                     GWDNQWKGDDDYLKWLDQCLAQFWRVLKPAGSLYLFCGHRLASDIEIMMRERFSVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERILFAEHYQGPYRPKDAGYEAKGRALKQHVMA
                     PLIAYFRDARAALGITAKQIADATGKKNMVSHWFSASQWQLPDESDYLKLQALFARVA
                     EEKHQRGELEKPHHQLVSTYSELNRQYTELQSEYKHLRRYFGVTAQVPYTDVWTHKPV
                     QYYPGKHPCEKPAEMLQQIISASSRPGDLVADFFMGSGSTVKAALALGRRATGVELES
                     ERFLQTVKEIRDDFCR"
     gene            44994..45716
                     /locus_tag="ACHKIR_001788"
     CDS             44994..45716
                     /locus_tag="ACHKIR_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO9989537.1"
                     /translation="MAVVCSVILVCSPINIFIEKDKLSLVPGMVILVTRNIRELFRTY
                     TDRVKVADINHDIVSQYLEGGRRRMSSPQACFPHYLTSGIHQPVLAEALITNHGFPSA
                     PSGDLSTMTGLSCLSLFANDESLSSFLSRCLSNTSDKVRAIIQSDISVNWKLGVIALQ
                     LHMSESLLKKKLKDEGNSFSRLLLEERMRGAVNMLCFQYGRGAIVAEKCGYSSLSYFI
                     SVFHRYYGVPPDKYVSLHMVDF"
     gene            46794..>47155
                     /locus_tag="ACHKIR_001789"
     CDS             46794..>47155
                     /locus_tag="ACHKIR_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFO9989538.1"
                     /translation="MAFKHYDVVRAASPSDLAERLTQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVTTPVVVKPSDGEGTVISATSDPEYYFVVVLAGQSNGMSYGEGLPLPETY
                     DRPDPRIKQLARRSTVTPG"
BASE COUNT        12105 a        11059 c        12404 g        11587 t
ORIGIN      
        1 taatcagggc tttattttaa gccctggttg ttttatattc atcgctgttg cttatttaga
       61 cattttgtac ttttattgtt gaggttatta agcgaagcga caatggattg tgttgctgcg
      121 gttttatagg gtaggggaga ggcagatgcg aattaaacca gacgataact ggcgttggta
      181 ttacgatgaa gagcacgatc gaatgatgct cgatttagcc aatggtatgc tatttcgctc
      241 acgttttgcg cgcaaaatgt tgactccaga tgccttttct cccgcaggct tttgcgttga
      301 cgacgccgcg ctctatttct catttgaaga aaaatgccgc gattttaatt tatctaaaga
      361 acaaaaagcc gaactggtgc ttaatgcgct ggtagcaatt cgttatctca aaccgcaaat
      421 gccgaaaagc tggcattttg tttcccatgg tgaaatgtgg gtgcccatgc cgggcgatgc
      481 ggcctgcgtt tggttgagcg acactcacga gcaagtcaat ttgctggtcg ttgaatctgg
      541 cgaaaatgcc gcactatgcc tgctggcaca accctgcgtt gttattgcgg gtcgcgcgat
      601 gcagctgggc gacgccatca aaatcatgaa cgacaggctg aaaccgcagg ttaatgttga
      661 cagcttcaac ctcgaacagg cagtctaacg cgtcagctta agcgcagtct tcggtacaca
      721 gctacagcaa agaatggtgc catcatcgcc cattgctgat tttttcagcg gcgtgacttc
      781 gccttctaaa agctgaacac ggcaacttcc acaaatgccc gcgcggcaag aataagggat
      841 acgaattccc tgattttcta attgctccag caacacctgc tggttatttc cacgaaatgc
      901 ctgtccctgc cagtcaatat ctacatttgc gtccggttgt tgcgtgatgt tgacggtatc
      961 atcagcggcg gctgcgccgt aaattttagc cggagccgtt gccagaactt ccacctcatc
     1021 gccaacgcga attacgccgc tattacgggc aattaaattc tgaccaaaat cgacatcgcc
     1081 gttatcctgg gcagtgcgga aagattgcaa tgtttttaat ggttcgcctg ccggatgttt
     1141 ttgccctttt tctggactga cggtggtgaa aatacagcgg ctacaaggtt taaccacatc
     1201 aaacaccaca tcaccaatgc gaatcacttt ccagctatct tcttcccagg ctgacgcgcc
     1261 ggaaaccacc agattggggc ggaattgctc catttttaca ctggccggac aacgttgttg
     1321 gagatcacgt aacgaggctt cgttagcaag aaggtaagga tagccatcag caaatgacag
     1381 gggtacagtg ttgtggcgtt tcacgcgccg cgtcatttgt ggccccaccc agcgcaactg
     1441 cacttcgcgg gagaaaaatc cacttagcca tttgttgatc gcgtctggtg caattcgcgc
     1501 ggtaaaatgt gtgccccaaa cttcggttgg tgcgtcttgt gtggcgaaat cagcaaaacg
     1561 aacatatgcg ctactgccat ctggtgcggt gagatgcaag ccatcatgca caggtgaagg
     1621 agtaaaccgt accatctggg gaaactggcg agcggtaata aacgtaccgt caggttccgt
     1681 gatcataaag atgcgatcga aggccagacc actgacatct gccagggcat gtgtaagacc
     1741 aatgccgcgc atcgatttaa caggatgaat aaaaagccgg gttaatgtcg ccacggtgcg
     1801 gtcctcaaat gaaaataagc cctcaacttt atgacatgac gcgcttatta gctataatgc
     1861 gcaacaattt tcttagcgcc tgtcccatta ggctatttta tttgtcagtt tggcccaggg
     1921 aagtgctcga aatcctcacg tacttgtgta cgctccggtt tctccgcgct gtccatgtcc
     1981 agactgactg caacaattac gcctactgcg gtaggctctt agagtaaaag tgacgatatg
     2041 aattctctgt ttgccagtac ggcccgtggg ctggaagagc tgttaaaaac tgaactggaa
     2101 aacctggggg ccgttgaatg ccaggtggtt cagggtgggg tccatttcaa gggcgacaca
     2161 cggcttgttt accagagcct gatgtggagc cgcctggcct cgcgtattat gttgccgctg
     2221 ggcgagtgta aggtttacag cgatttagac ctctatctcg gtgttcaggc gatcaactgg
     2281 acagagatgt ttaatcctgg cgcgaccttc gctgtccact tcagtggttt gaatgacacc
     2341 atccgcaaca gtcagtacgg cgcgatgaaa gtgaaagacg cgatcgtcga tgctttcacg
     2401 cggaaaaatc tgccgcgtcc aaatgttgat cgcgatgcgc cggatatccg cgttaacgtc
     2461 tggctgcata aagaaaccgc cagtattgcc cttgatctca gtggtgatgg tttacatctg
     2521 cgtggctatc gcgatcgtgc tggtattgcg ccgatcaaag aaaccctggc agccgcgatt
     2581 gttatgcgat ccggctggca gccaggaaca ccgctgctcg atccgatgtg tggttccggt
     2641 acgttgttga ttgaagcggc gatgctggcg accgatcgcg caccaggctt gcaccgtgga
     2701 cgttggggct ttagcggctg ggcacagcat gatgaagcca tctggcagga agtgaaagca
     2761 gaagcgcaaa cccgcgcccg taaaggcctg gctgagtata gctctcattt ttacggttcg
     2821 gacagcgacg cacgggtgat tcaacgtgcg cgcactaacg cccgtcttgc ggggattggt
     2881 gaactgatca cctttgaagt gaaagatgtc gcgcaactga ccaatccgct gccgaaaggg
     2941 ccgtacggta cagtgttgag caacccgcca tacggtgaac gtctggacag cgaaccggcg
     3001 ctgattgcgc tgcatagtct gctgggccga atcatgaaaa accagttcgg cggctggaat
     3061 ctctctttat tcagtgcctc gccggatctg ttaagttgcc tgcagttgcg tgcagacaaa
     3121 cagtacaaag cgaaaaacgg tccattagac tgcgtgcaga aaaattacca cgttgccgaa
     3181 agcacaccag acagcaaacc ggtaatggca gcggaagact acgccaaccg tctgcgtaag
     3241 aacctcaaaa aattcgagaa gtgggcgcgt caggaaggca ttgaatgcta ccgcctgtac
     3301 gacgccgatc taccggaata caacgttgca gttgaccgct acgccgattg ggtggtggtg
     3361 caggagtatg cgccgccaaa aactattgat gcgcacaaag cgcgtcagcg tctgttcgat
     3421 attatcgctg caaccatttc ggtattgggg attgcgccaa acaaactggt gctgaaaacc
     3481 cgtgaacgcc agaagggcaa aaatcagtac cagaaactgg gcgagaaggg cgagtttctc
     3541 gaagttaccg aatataacgc tcacttgtgg gtgaacctga cggattacct cgataccggt
     3601 ctgttcctcg atcaccgtat cgcccgtcgt atgctcggtc agatgagcaa aggcaaagat
     3661 ttcctcaacc tgttctctta taccggcagc gccaccgtgc acgcgggatt aggcggtgca
     3721 cgcagcacta ccaccgtgga tatgtcgcgt acttatctgg agtgggcaga acgcaacctg
     3781 cgtctgaatg gcctaaccgg gcgtgcgcat cgcctgattc aggccgattg cctggcgtgg
     3841 ctgcgtgagg caaatgaaca gttcgatctg atttttatcg atccgccaac cttctctaac
     3901 tcaaaacgaa tggaagatgc gtttgatgtt cagcgcgatc atctggcgct gatgaaagat
     3961 ttgaaacgtc tgctgcgtgc tggtgggacg atcatgttct cgaacaacaa acgtggcttc
     4021 cgtatggatc ttgacggact ggcaaaactg ggactgaaag cacaagaaat tacgcaaaaa
     4081 acgctctccc aggatttcgc ccgtaaccgc cagatccaca actgctggct gattaccgca
     4141 gcctgaaagg aatagtaatg tcattaatca gtatgcatgg cgcatggctg tcgttcagcg
     4201 acgcgccgct tctcgataac gcagaactgc atatcgaaga taacgaacgt gtttgtctgg
     4261 tgggccgcaa cggcgcaggc aaatcgacgt taatgaaaat cctcaaccgt gaacaagggc
     4321 tggatgacgg tcgcattatt tacgagcaag atttgattgt agctcgtctg caacaggatc
     4381 cgccgcgtaa cgttgagggg agtgtttatg atttcgttgc cgaaggcatt gaagaacaag
     4441 cggaatacct gaaacgctat cacgatattt cgcgcctggt gatgaacgac ccgagcgaga
     4501 aaaatctcaa cgaactggcg aaggttcagg aacagttgga tcaccacaac ctgtggcagc
     4561 tggaaaaccg cattaacgaa gtgctggcgc aactggggtt agatcctaac gttgcgctgt
     4621 cgtcgctttc cggcggctgg ttgcgtaaag cggcattagg acgcgcgctg gtgagcaatc
     4681 cgcgcgtatt gctgctcgac gaaccgacta accacctgga tatcgaaacc atcgactggc
     4741 tggaaggatt ccttaaaacc ttcaatggga cgataatttt catctcccac gaccgttcgt
     4801 ttatccgcaa tatggcgacg cgcattgttg atctcgatcg cggcaagctt gtgacctatc
     4861 ctgggaatta cgaccagtac ctgctggaaa aagaagaagc cctgcgcgtg gaagaactac
     4921 aaaatgccga gttcgatcgc aaactggcgc aggaagaggt gtggatccgc caggggatca
     4981 aagcacgccg tacccgtaat gaaggccgcg tacgcgccct gaaagcaatg cgtcgtgagc
     5041 gtggcgaacg tcgcgaagtg atgggtaccg caaagatgca ggtggaagag gccagccgct
     5101 ccggtaagat cgttttcgaa atggaagacg tttgctacca ggttgacggt aagcaactgg
     5161 tgaaagattt ttctgcccag gttctacgtg gcgacaaaat tgccctgatt ggtccgaatg
     5221 gatgcggcaa aaccacgctg ctgaaactga tgctcggtca gcttcaggcg gacagcgggc
     5281 gtattcacgt tggcaccaaa ctggaagtgg cttatttcga tcagcaccgt gcggaactgg
     5341 atcccgataa aacggtgatg gataaccttg ctgaaggtaa gcaagaggtg atggttaacg
     5401 gcaagccacg tcatgttttg ggctatttgc aggacttcct gttccatccg aaacgggcga
     5461 tgacgccggt acgtgcgctt tctggcggtg agcgtaaccg cttgctgttg gcgcgtttgt
     5521 tcctcaaacc aagcaactta ttgattcttg acgaaccgac caacgatctt gatgttgaaa
     5581 cgctggaact gctggaagaa ctgatcgaca gctaccaggg cacggtattg ctggtgagcc
     5641 acgatcgtca gtttgtcgat aacaccgtta cagaatgctg gatcttcgaa ggcggcggta
     5701 aaattggtcg ttatgtcggc ggttatcatg atgcccgtgg tcagcaagaa cagtatgtgg
     5761 cgctcaaaca gcctgcggtg aaaaaaatcg aagaagccgc cgcgccaaaa gcagaaactg
     5821 taaaacgcag cagtagcaaa ctaagctata aattgcagcg cgaactggag cagctaccgc
     5881 aattgctcga agatctggag gcgaagctgg aagccctaca gacgcaggtg gcggatgctt
     5941 ccttcttcag tcagccgcat gagcagacgc aaaaagtgct cgccgatatg gctgctgcag
     6001 agcaggagct ggagcaagcc tttgaacgct gggagtatct tgaagcgtta aaaaatggtg
     6061 gctgatcgca aaatagtcga tttaaacaga gacagctgac aacggtaaaa ttgcctgatg
     6121 aactgggttt atcaggcatg aaaaccaaca ctttgttagt catctgtaaa atagcgcatc
     6181 attaaggagt accaatgtgc gaacatcatc atgccgcgaa gcacatcctg tgctcgcagt
     6241 gtgacatgct ggtggcgtta ccgcgccttg agcatggtca gaaagcggca tgtccccggt
     6301 gtggcacaac gttaaccgtg gcgtgggatg cccctcggca gcgtcccacc gcctatgcgt
     6361 tggctgcact gttcatgctg ttgctgtcca acttgtttcc ttttgtgaat atgaacgttg
     6421 cgggagtcac cagtgaaatt acattactgg aaattcccgg cgtgcttttt tctgaggact
     6481 acgccagcct cggcaccttt ttcctgttgt ttgtgcaact ggttcccgcg ttttgtctga
     6541 taaccattct gttactggtg aatcgcgcag aattaccggt ccgtttaaaa gagcaactgg
     6601 cacgggtgct ttttcaactc aaaacctggg gaatggcgga gattttcctc gcgggtgtgc
     6661 tggtcagttt cgttaaactg atggcttacg gcagcattgg cgtaggcagc agctttctcc
     6721 cctggtgttt attttgtgtc ctgcaactgc gtgcttttca gtgcgttgat cgtcgctggt
     6781 tatgggacga catcgccccg atgccagaac tgcgccagcc gctaaaacct ggcgtcacgg
     6841 ggatacgcca ggggttacgt tcgtgctcct gttgtacagc aattcttcca gctgatgatc
     6901 cggtgtgtcc gcgctgtggt actaaagggt acgttcgtcg tagaaacagc ctgcaatgga
     6961 cactcgcgct gcttgttacg tccatcatgc tgtatcttcc ggctaatatt ttgcccatca
     7021 tggtgacgga tttattaggc tcgaagatgc catcgacgat tctcgctggg gtcatcctgt
     7081 tatggagcga aggatcttat cccgtcgctg cggttatctt tctggccagt attatggtgc
     7141 caacgttaaa gatgatcgcc atagcgtggt tgtgttggga tgccaaaggg catggcaagc
     7201 gcgacagtga aagaatgcat ttgatttatg aagttgttga gtttgtaggc cgctggtcga
     7261 tgattgacgt tttcgttatc gcggtgctct cggcgctggt gcgtatggga ggtttaatga
     7321 gtatttatcc ggcaatgggt gcattaatgt ttgctttagt cgtcataatg acaatgtttt
     7381 ctgctatgac gtttgacccg cgtttgtcgt gggatcgtca acctgaatca gagcatgagg
     7441 agtcctgaca gtatggaatc taataatggg gaagccaaaa tccagaaagt gaagaactgg
     7501 tctcccgtgt ggatatttcc tatcgtcacg gcgctcattg gggcctgggt tcttttttat
     7561 cattacagcc atcaggggcc ggaagtgacc ctgatcaccg cgaatgcgga aggaatagaa
     7621 ggtggtaaaa ccaccattaa aagccgtagc gttgacgtcg gcgtggttga aagcgccaca
     7681 ctggctgatg atttgacgca cgttgaaatc aaagcgcggc tgaattccgg tatggaaaaa
     7741 ttgctgcata aagataccgt cttttgggtg gtgaaaccgc agattggtcg agaagggatt
     7801 agcggcctgg gaacgctgct gtctggtgtt tatatcgaac tgcagccagg cgcgaaaggc
     7861 agcaaaatgg ataaatacga tttgctggac tcaccaccgt tggccccgcc tgatgcgaaa
     7921 ggtatccgtg tgattctcga tagcaaaaaa gccgggcagc tctcgccagg agatcccgtg
     7981 ctgttccgtg gctatcgggt aggttcggtt gaaaccagca cctttgatac gcaaaaacgc
     8041 aatatcagct atcaactgtt catcaatgca ccttatgacc gactggtgac cagcaatgtt
     8101 cgcttctgga aagatagtgg cattgcggtt gatttgacgt cagcgggaat gcgtgtggag
     8161 atgggttcat tgacaacgct gcttagtggc ggtgtcagct ttgatgtgcc ggaaggtctg
     8221 gatttagggc agccagtggc accgaaaaca gctttcgttt tgtatgatga tcagaagagc
     8281 attcaggatt cgttgtacac cgatcacatt gattatctga tgttctttaa agattcggta
     8341 cgcggtctgc aaccgggagc tccggtagaa ttccggggta ttcgcctggg taccgtaagc
     8401 aaagtgccat tctttgcgcc gaatatgcgt cagacattta acgatgatta ccgtattccg
     8461 gtactgattc gtatcgagcc agagcggctg aaaatgcagc ttggcgaaaa tgcggatgtt
     8521 gttgagcacc ttggcgaatt gttgaaacgt ggtttacgcg gatcgctgaa aaccggaaac
     8581 ctggtcactg gcgcactgta tgttgatctc gatttctatc caaatacgcc tgcaataacc
     8641 ggtattcgtg aatttaatgg ttatcagatt atcccgaccg ttagcggcgg tctggcgcaa
     8701 atccagcaac gactgatgga agcgttggat aagatcaaca aactgccatt gaatccgatg
     8761 attgaacagg caaccagtac gctttctgaa agtcagcgca caatgaaaaa cctgcaaacg
     8821 acgctggata gcatgaacaa gatcctcgcc agccagtcga tgcagcagct gccgacggat
     8881 atgcagtcaa cgttgcgtga attgaatcgc agcatgcagg gcttccagcc tggctccgca
     8941 gcctacaaca agatggtggc ggatatgcag cgccttgatc aggtgttgcg agaactgcaa
     9001 ccggtgctga aaacgctcaa tgagaagagt aacgcgctgg tatttgaagc gaaggacaaa
     9061 aaagatccag agccgaagag ggcgaaacaa tgaaaaagtg gctagtgacg attgcggcac
     9121 tgtggctggc cggatgcagc tccggcgaaa ttaataaaaa ctattaccag ttacctgtgg
     9181 tgcagagcgg tacacaaagc accgccagcc agggtaatcg cctgttatgg gttgagcagg
     9241 tcgctgttcc tgactatctg gcggggaatg gtgtggttta tcaaaccagt gatgtgaagt
     9301 atgtgattgc caacaacaac ctgtgggcca gcccgttgga tcaacagttg cgcaacactc
     9361 tggttgccaa cctgagcacg caactgcccg gctgggtggt tgcctcccag cctctgggaa
     9421 gcgcccagga tacgctcaat gttaccgtaa cggagtttaa cgggcgatat gatggcaagg
     9481 tcattgtcag tggtgagtgg ctgttgaacc accagggaca actgatcaaa cgtccgttcc
     9541 gtctggaagg ggtgcaaact caggatggtt acgatgaaat ggttaaagtg ctggccagtg
     9601 tctggagtca ggaagccgct tctattgcgc aagagataaa gcgtctaccg taatcataaa
     9661 gatttgtaaa tataaccgtc tccggtatgt tgcctgaggc ggtttttttg tttctaaggt
     9721 gcggaaaaag ttgttcctct tcacattttt tgtacaactg acatgcccgt gtagctcaca
     9781 aatatgacag tggcgtgaat tttgcgcatt gacggcagtt atgattcgcg gtattgctta
     9841 actgtgattg cacatttagt aatcactgtt ttcttttcca ccagaaacca gtatgaggga
     9901 aacgaggcat gaagagacaa aaacgagatc gcctggaacg ggcacatcaa cgtggttatc
     9961 aggccggcat cgccggacgc tcaaaagaaa tgtgtcccta tcagacgctg aatcaaaggt
    10021 cacaatggct gggaggctgg cgagaagcca tggcggacag ggtagtaatg gcctgattct
    10081 gtctctttaa aaagaaacct ccgcattgcg gaggtttcgc cttttgatac tctgtctgat
    10141 tataatcaga aggcagacgt atcctggaac agaccgactt tcaggtcgct ggcggtatag
    10201 atcagacgac catcaaccag cacttcgcca tctgccagac ccataatcag acgtcgatta
    10261 acaatgcgtt taaagtgaat acggtaggtc acttttttcg ctgtcggcag tacctgaccg
    10321 gtgaatttca cttcgccaac gcccagcgcg cggcctttac cttcgccgcc cagccagccg
    10381 aggtagaacc ctaccagctg ccacattgcg tccaggccca ggcatcccgg cataaccgga
    10441 tcgccaatga agtggcatcc gaagaaccac agatccggat tgatatccag ttctgcttca
    10501 acataccctt tgtcgaagtt accacccgtt tcggtcattt tgaccacacg gtccatcatc
    10561 agcatgttcg gtgctggcaa ttgcgggcct ttagcgccaa acagttcacc gcgaccagag
    10621 gcaagaaggt cttcttttgt ataggattcg cgtttatcta ccatgttctc tgtaagcctt
    10681 attttattga agcacgcagg atagctaaca cgtgtacgct gaacaagtcc gatcagttcg
    10741 gaataaacca gttcagccaa cgcaatggcc atggaaaacg gtgacgtcct tcctgttgcg
    10801 atgcttgcgc gatacgttcc tggatggttt gcatcagcgt cgtttggcct tcgccatccc
    10861 acaccagatt taataataac ggcagtgcgt cagtcacatc gtctactgcc cagatggtga
    10921 atttgccttc ttctaccgct ttcaccagtt cactgtgaag acttaaatgg cgaatgttag
    10981 cagtggggat aatgacacct tgtttcccgg ttaactcacg ttgctggcaa atagcaaaga
    11041 agccttcgat tttctcattt aaaccaccga ccggctgggc acgaccgaac tgatcgactg
    11101 aacctgtgat agcgatactc tgattcaccg gcacatcggc gagggcgctt atcagggcgc
    11161 agagttcagc catcgaggca ctatcgccat ccacttcact gtatgactgc tcaaatgtca
    11221 gcgatgctga gaaggggatc tgttgctcaa gctgtagttc cgacatcagg aacgcctgca
    11281 tgatcatcat ccctttcgca tggatattgc cgccaagctc cgctttgcgt tcgatgtcgg
    11341 tgaactcacc atcgccaata tgcacaacgc agctaatgcg agaaggttcg ccaaaagcgc
    11401 gtggatgacc cggaaattca atgaccgaaa gggcgttaat ttgcccgatg cgttcgcctt
    11461 cggtttcaat caggatttgc tcctgaagaa tctcatcctg catgcgttca gcgaggaaac
    11521 cttcacgcca ttcacgctgc tgcagcatta agtttagctg ctcgccggag aaggtgtcgc
    11581 catcgcacag ggaggcgacc tctttacact ggcggaggat ccactgcggg ctaagcggaa
    11641 gtgtttcttg ttcaccggtg tagcgggctg cttcgcggat aagtaccggc caggcatccg
    11701 ctcccggtgc aggtaagtga ttatgtcttg cggtcaatgt tacccagcga caccactggc
    11761 ttactgactc cgcatcgaca atctgcagag tatcttcaaa ttcgctataa atagcctgct
    11821 ctgaaagctc tggttccatc tcctggaaat cagccaatga ttcgcgttcg cctaccagaa
    11881 tgactttcag cttcaatggc atcgaaggca cagagacggg gagagggcgc gactcatcaa
    11941 acgcaaccca gtcaaaacgc tcgcggttaa cgatattttt cagccgcatc cacagcagag
    12001 gttgcgccag cagtgtacgc aaagagatga tgagaatacc gccatttgcc tgatgcacca
    12061 gacctggctg cagggtaatg tcgccattaa actggcgcag gcagccaaag agttgttccg
    12121 cttctaccca gtcggcagcg acaacttgcg ttaaagtcgc aaaattatca tctgcactca
    12181 ctgcgttacg taagcggatg gtgtgcccgg aaacctcata gtgaccgccc accagttggc
    12241 ctgcatcgct ttgtagcgca cgcgcggcat cggcaataag attcagatac tcagactctt
    12301 ccggggcctt cgccagcata aaagaggagg atgctcgcgt atgcagcaac tgctccagcg
    12361 caaattgcag tcgcggttga gtatcactga ataaaggatc gttttcgtca atcaaatgtg
    12421 gctgagcaaa tatttcctga tagctatcgg tatcaggaac caggtcacgc catgcaagtt
    12481 tcgtaatggt caaagttgat gttttttagt ctgttgtcaa agccgcgatt ataccgtaac
    12541 cggcactaca gcacacgtag aaagcaccga caatactcct ggcatgggcg ttaaagctca
    12601 caggatggag attcttttct tcactggccc aaaaagctga tattctgtaa agagttacac
    12661 ggtaacattg agatcgctat gaaatatcaa caacttgaaa atcttgaaag cggttggaaa
    12721 tggaagtatc tggtgaagaa gcaccgcgaa ggggagttaa tcacccgtta catagaagcc
    12781 agtgccgccc aggaagccgt cgatgtgttg ctctcgctgg aaaatgaacc cgtgctggta
    12841 aatggctgga ttgacaagca tatgaatccg gaactggtca atcgcatgaa gcaaaccatt
    12901 cgggccagac ggaagcgcca ttttaatgcg gaacatcagc atacgcgcaa aaaatcgatc
    12961 gatctggaat ttattgtctg gcaacgtctg gctggtcttg cgcagcgtcg cggtaaaacg
    13021 ctttctgaaa cgattgttca gctgattgaa gatgcggaaa acaaagagaa atacgcgaat
    13081 aaaatgtctt ctttgaagca ggatctgcag gcattgctgg gtaaggaata actgacgaaa
    13141 gtcagttcaa ttttctaaag acgaaaaaaa accccgcagc agcggggttt tttaccagac
    13201 gagaacttaa gcctgcggct gagttacaac gtctttgatg cctttaactt cgatctctac
    13261 gcgacgatcc ggagccaggc aatcgatcag tgcagcacgc tgtttcacgt tgtcacaggt
    13321 gttgccagta accgggttgg attcgcccat accacgtgcg gagattttgt ctgccgggat
    13381 acctttagag atcaggtaat caacaacgga ctgagcacga cgctcggaca gagcctggtt
    13441 ataagcgtca gaaccgatgc ggtcagtgta acccagaaca actacggaac cgtctttcgg
    13501 atccaggttg ctcagctggc tgtacagctg atccagagca gcctgacctt ccggtttcag
    13561 ggttgctttg ttgaaggtga acagaacgtc agacttcaga gtgaagtgct tggtctgtac
    13621 ttccggtgcc ggagctggag ccggagcaac tactggagct gcttcgccct gaccgaaacg
    13681 gtaggaaaca cccaggctca gcatgccgtt gtccggacga gtgccgatgg tgtgtgcgtc
    13741 accgatgttg ttggtccact ggtattccag acgggtagcg atttcaggag tgatcgcata
    13801 ctcaacaccg ccagcgaaga ccggagaaac gccggtgtcg tggtctttag tggatgcgcc
    13861 accaggtacg ttagacttgg tgtctgcacg ccataccata ccacccagac gagtgtaaac
    13921 gtccagatcg tcagtgattg ggtaacccag tttagcggtc agctgaacgc cctgagcttt
    13981 gtatgcgccg ttgatgttgt cgcctttgta cggcatacga cctaaccagt cgtaacccat
    14041 ttcaaagcca acatacgggt taacctggta accaccaaaa gcacctgcac ccagttggtt
    14101 ttcgtgggtc ggaccattgt taggaataaa accagtgtca tggtactggg accagcccag
    14161 tttagcacca gtgtaccagg tgttatcttt cggagcggcc tgcgctacgg tagcgaaacc
    14221 agccagtgcc actgcaatcg cgatagctgt ctttttcatt ttttgcgcct cgttatcatc
    14281 caaaatacgc catgaatatc tccaacgaga taacacggtt aaatccttca ccggggatct
    14341 gctcaatatt aactctaccg atatcttcgg cttatgccga gcacccctgg cgatgtaaag
    14401 tctacaacgt agttgaaaac ttacaagtgt gaactccgtc aggcatatga aaaaaaagtc
    14461 ttgtataagg tacgtttaat cttttttgtc agcgacaatt tacagaagaa aatcgcggaa
    14521 accgcttcag acaagcctcc gcaaggaaaa ttagtcacga ctgaaagcat tggctgggcg
    14581 acaaaaaaag ttccaggatt aatcctaaat ttacttaatg atacaaatta gagtgaattt
    14641 ttagcccgga aagttgtctc gttgcgtgag aggatgcgct taccggacgc ataataaacc
    14701 ccatagcgtt accttcattt gccgcatcaa caagttcagc atgctcttct tcagtcaaat
    14761 catctgccaa ccaaccgatc accacgctgt aattgcccgt gcgtaaagcg cgaaccattg
    14821 actcaacggt gtggcaaggg gagagctggc taatctgcat tactttcgtt aagggtagcc
    14881 cagatgcctg aacccattcg cgactcagtt tttgttgcgg tgttaaccag agttgccagc
    14941 gcgattgctg accgagttgc tgtaacaatg gcaacagtag aagttgcgtc atcatgggct
    15001 gatcttcgcg atagacaact tcactgataa gcccggctgt agtgttttcc gtagagacac
    15061 gcgcaatttt acttgctgcg gatgagaacg acgaagcacg atgtgcatag cctgaagtgt
    15121 acataatcaa tccagccctg tgggttactg tatgaatata cagtacatcc ggtgacgaca
    15181 aagatcaacc ctattttcgg aaagagcctc gcaaattttg tcgttagtga cgggaaaaca
    15241 taaattaatc ttgcccctta agaataagtt gcctattttc gtagttaacg gatccgttaa
    15301 tgtgaatcat tcttttatgt tatgatttta aaaggaattt tatgaaaagc ctctcctata
    15361 agcggatcta taaatcacaa gaatacctgg caacgttggg cacaattgaa taccgatcat
    15421 tgtttggcag ttacagcctg accgttgacg acacggtgtt tgcgatggtt tctgatggtg
    15481 agttgtatct tcgggcttgt gagcaaagtg cacagtactg tgtaaaacat ccgcctgtct
    15541 ggctgacata taaaaagtgt ggccgatccg ttaccctcaa ttactatcgg gttgatgaaa
    15601 gtctatggcg aaatcaactg aagctggtgc gtctgtcgaa atattctctc gatgctgcgc
    15661 tgaaagagaa aagcacgcgc aatacccggg aaagactgaa agatttgccc aatatgtctt
    15721 ttcatctgga agcgattctc ggggaggtgg ggattaagga tgtacgggcg ttacgtatac
    15781 ttggggcaaa aatgtgttgg ttgcgactgc ggcagcaaaa cagtctggtg acagaaaaga
    15841 ttctgtttat gcttgaaggg gccattatcg gcattcatga agctgcgctc ccggtggcac
    15901 gccgccagga gcttgcagaa tgggctgact ctcttacgcc gaaacaggag tttcctgcgg
    15961 aacttgagta atctggcgtt gcagacgacc aatctctggc agtaatgcaa tcaataaccc
    16021 aacttgttgt acgaccagag gttctttgct gtctgcccgt ggttcaagtt gctgcatccg
    16081 ctgtttcagg ccagctaatg cctgattgac gcgttcttca tcagcaggtt gatgatgtaa
    16141 cgcgtcatca acatagcaaa ctgcgtcatc aagaaacgcc agaatttcag gattagttaa
    16201 ctgctcccgg tgagcaccga gggctgagat atagctggta aacgtatggt taaggcacag
    16261 caaccgaaac gcggcttcgc gaatttgcgg ggtaacgttc ggttcgctgg acatatttga
    16321 taccaccgac gccagttcag catcacggtt gtgtgcatcg cggcgggcaa tacgatacgc
    16381 cagacggtta tcacgcccct gatggtattg ctccagtatg gcatcgagat accggcagtt
    16441 ggcctcggtg gcgcgttcga gcattcgcgg cagattgcga aacttccagt caggccagat
    16501 gtagctcact gccgcccacg caatggcaca accaatcagc gtatcgatta cgcgaggtaa
    16561 cgccacttca aaaccttcac ccagcaagtt gaaacacagt agcaccaaaa gtgtgatgaa
    16621 catcgttgca tgggcgtatt gcacgttacg gaaggcaaaa aagagcacgc cggtaataac
    16681 cagcagcacc agctgccctt ccagtgatgg cacaaaccac agcacaggaa taccaatcgc
    16741 gatacctacc agcgtaccaa taatccttaa cttcaggcgg tggcgcgtgg cgttatagtt
    16801 tggctggcag acaaacaaac ttgtcagcaa gatccaatac ccgtgatgca ttccggttat
    16861 ctgaatgatg gcgtagccga agcacaacac cagcgacatt cttaccgcat gacggaagag
    16921 ggcggattcc ggcgtgaagt gacggctaag acgcagccag atatcactca atccgtgcgg
    16981 gctgtcatca gcgagctcat tttcgtcatt attatggggc agtgcctggg cctgttctga
    17041 ttcaattgtt gccagttggg catcaatggc acgtaaattg ttcagtaaaa atcctagtgt
    17101 tttgagtaaa tcagcaggag cgccgttatc gcgcatccgc tccagcgcag catcaatatg
    17161 cgtaaaagcg cgctcaaaat gcggatcatg ttgataaggc tgacgcagca aaatacagcg
    17221 tgacagttgc tggcacgcct ggccctgcat cgacatcagc cgctgaaaac ggaacagcac
    17281 gtcgctgtgg cgaaaatgtt cacgcaatgt ttgatattga atatgagaag agctggcacg
    17341 ctcgtgaata tcctgcgcga caaagtaata atgcagtgtg cgacgcgttc ctcgttgacc
    17401 acgatcgcca cgtaagcggg tcagcagcga gagtttcgtc tgattcaatg tcgccatcag
    17461 ctgaccgttg gcgagagcca aatcgtacag cggtgcctgg ctttcatctt caatatcagg
    17521 atcaaacatg cgcgacttga gctcaagata acgcgccagt tgttcatagc aacgcgccag
    17581 gttgtcctgc agcgggcgga ccgggaacag cagatgacca ataagtgtca ggacgttgta
    17641 ccagacggca ccggccagca gatacatcgg ctgctgatac cagtgctcat acagtgacgt
    17701 ccccaacata gtgtaaatgg cgatcagtaa cgcaccgaag gcaattgttg catagcgttg
    17761 acccagaccg ccgagcaaaa tgaagccgct ggtagagagc gttaaaccaa ttgcaaatag
    17821 ccagggccag ggaaacagca attctactga ggccgaggcg ataaaaaagc agaacagcgt
    17881 aatgatgagg ttacgcaaac gtcccgccag tcggtcatcg agatcggtca gcgccgctgc
    17941 caccatcccc agcgttagcg gaatcgtcag ttttacatca cccagccacc acggaaacgc
    18001 tgtggttcca caaagcgcaa taaaaatacg cgcgtaatac agccaggcgc tgttccaggt
    18061 atagcgtttg agcaaaggac ttagcataaa ggccatatta gtggattatt caaaacgacg
    18121 acgtgcattg gcttcacgcg cagcttgcgc tgtttctacc gataccacgc gacgaccgac
    18181 cggccatagt gcaatagcgg caattttaaa gttcgcaatg ccgacaggaa tgccaatgat
    18241 ggaaatacat tgtgcgatgc ccgttgcaat gtgcatcagg cataaccacc agccaaagaa
    18301 aatcagccag aaaatattca ataccgtacc gccagtattt agcagcacat ttttgccagc
    18361 cgggttcagt tcatcgacat gaatagcttc attgccataa ggcaccagag acagtttagt
    18421 gatctcccag caggatcgtg tcagcggtaa ggtaaaaatc agcacaatac tgaccagagt
    18481 cgccaacagc cagcccagag tggtggcaaa tccgccaagc acaaagttca gaatgttcaa
    18541 aacggtacgc ataaaacctc gctttactgt ggttttcagt aatgggcggc aattgtaacg
    18601 tttttttggg ctggagcacg ttttctctga cggttacact gataagaaat aatttcgtgt
    18661 ggatctacag agtcatggaa ctgaaagcga caacgcttgg aaaacgtctg gcacagcacc
    18721 cttacgatcg ggcggtgatc ctcaatgccg ggattaaagt ctccggcgat cgccacgaat
    18781 accttattcc tttcaatcaa ttactggcga ttcactgtaa gcgcggtctg gtatggggcg
    18841 agctggaatt tgtactgccg ggcgaaaaag tggtgcgtct gcacggtacc gaatggggcg
    18901 agacgcagcg tttttaccat catcttgatg ctcactggca gcggtggagt agcgagatga
    18961 gcgaaattgc atctggtgtt ttacgccagc aactggattt gattgccacg cgtactggag
    19021 aaaataaatg gctgacgcgt gagcaaacct ctggcgttca gcaacaaatc cgccaggctt
    19081 tgtcggcgtt gccgttgccg gttaaccgac tggaagaatt cgataactgc cgtgaggcat
    19141 ggcgtaaatg tcaggcctgg ttgaaagata ttgaaagcgc tcggttgcag cataaccagg
    19201 cgtataccga agccatgctt accgagtatg cggatttttt ccgccaggtc gagtcttcac
    19261 cgctgaatcc ggcgcaggcc cgggcagtcg ttaatggcga gcattctctg ttagtgctgg
    19321 ctggtgcagg aagcggaaaa acgtcggtgc tggtggcccg tgcaggctgg ttgctggcgc
    19381 gtggtgaagc gtcccctgag caaattttat tgctggcgtt tggtcgcaaa gccgctgaag
    19441 agatggacga gcgtattcga gaacgactac ataccgaaga cattaccgca cgcacatttc
    19501 atgcgctggc gctgcatatt attcagcagg gcagcaaaaa agttccgata gtcagcaaac
    19561 tggaaaatga taccgctgcc cgtcatgaac tttttattgc tgagtggcgc aagcaatgca
    19621 gcgaaaagaa agcgcaggcg aagggctggc ggcaatggct gacggaagaa atgcagtggt
    19681 cagtgccaga aggtaatttc tgggatgatg aaaaattaca gcgtcgtctt gcctctcgcc
    19741 tcgatcgctg ggtaagtctg atgcggatgc acggtggtgc acaggcagaa atgattgcca
    19801 gtgcacccga agtgattcgc gatctgttca gtaaacgtat caagttgatg gccccgttat
    19861 taaaagcctg gaaaggtgcg ctgaaggcag aaaacgctgt cgatttttcg ggccttattc
    19921 accaggcgat tgtgattctg gagaaaggtc gctttatcag cccgtggaag catattctgg
    19981 ttgatgaatt tcaggatatc tcgccgcagc gcgcagcgtt gttagcggca ttacgcaagc
    20041 aaaacagtca gacgacgttg ttcgccgttg gtgatgactg gcaggcgatt tatcgcttca
    20101 gcggtgcgca aatgtcgctc accaccgctt tccatgaaaa ctttggtgaa ggtgatcgct
    20161 gcgatttaga cacgacttac cgttttaaca gtcgtatcgg tgaggtggca aaccggttta
    20221 ttcagcagaa cccaggccag ctgaaaaagc cgctaaacag cttaaccaat ggagacaaaa
    20281 aagccgtcac gttattggat gagagccatc ttgacgcttt gctggataag ctctctggtt
    20341 atgccaaacc ggaagagcgc attctgatcc tggcgcgtta ccatcacatg aggcctgcca
    20401 gcctggaaaa agcggcaaca cgctggccga agttgcaaat cgactttatg accattcatg
    20461 ccagcaaagg gcaacaggcg gattacgtca tcatcgttgg cttgcaggag gggagtgatg
    20521 gttttccggc tgcggcgcgg gagtcgatta tggaagaggc gctactgcca tcggttgagg
    20581 atttcccgga cgctgaagaa cggcggttaa tgtacgtggc gctgacccgg gcacgccatc
    20641 gggtatgggc actgtttaac aaagagaatc cctctccatt tgtcgaaata ctgaaaaatc
    20701 ttgatgtgcc ggtggcgaga aaaccgtaag aaacaggtgg cgtttgccac ctgtgcaata
    20761 ttacttcaga cggtccgcga gataacgctg ataatcgggg atcagaatat cgaccgcgtc
    20821 gttgaaatgc ggcgactgga taatgaagtc tgccgttgcc acgttggtgg cgaccggaat
    20881 gttccatacc gtcgccagac gcagcaaggc tttcacgtca gggtcgtgcg gcacggcgtt
    20941 tagtgggtcc cagaagaaaa tcaatacatc aattttccct tctgagatca atgcgccaac
    21001 ctgctggtca ccccccattg ggccactcaa catcgcgttg acgttcatgc cggttgcacg
    21061 ggaaattaag ttaccggtag tgcctgttgc atacagtacg tgttgttcca gtaacggttg
    21121 atgccgttcc acccagctca tcagcatttg tttgcagtga tcgtgtgcca ccagcgcaat
    21181 atgtttccgc gcaggtaaag tgcgtgtcgt cagttccata atgtacatcc gtagttaact
    21241 ttcctacaga ttactgtaag cacttatcgc tgcaagataa agaccgaaaa agcctgcgca
    21301 caggcacaaa aatctcagga agatggttgt ttttccgccc attgcaggaa agtatttcgc
    21361 gtttgtgggt cagccagttt aaaccaatac ttcagccgtt gttctgtgag cacctgagac
    21421 tgcggtggaa tagcatccag ctcggaaaca cccgaaagca gcgtactatc atctgccatc
    21481 atcgtggcaa attgcggtag tgaagcgcgt ttcgcggatt tgttatagcg ttcaacctcg
    21541 acctcataat caatcgtttt tgccgttgag gtaatggcga gaatatccag ctttaccgga
    21601 atcggcgtcg catcgccatc caacaattca aggcgcggcg cggcatcaaa atggttagct
    21661 tcccgctcat tctccagacg aggcaggcga aaattgacct ggttgatgag ctgggtatta
    21721 aaactgacca ccagcggtgg ggagatatag agccgttctt cactattgga aagatgaatc
    21781 gtcttctcaa cgcgaaacac taactgatgc ggtccgttat ccagttcaat gctgtccgca
    21841 ccgtgcagca gagagctgga aaccttcttg ccgtcgagca ccagaagatc gacatcggtt
    21901 gaaagccgca gcgtggtggc aaaaacggat accggcaaac ataatgcaat caaggtggtc
    21961 acgatgccgg ttttcatagg tagctcctgt caaaagaccg cccgaataat aatgtgtcaa
    22021 aatttttctt taatcatact tactaacata tagacatatt tcccgctttt gctctcattc
    22081 attcgtatta gctgcatggt tggcatgtcg gctttgtcgt acactttgtt aaacagtctg
    22141 gaggaaataa tgaaagaaac cgatattgca ggcattttaa cgtccactca caccattgcg
    22201 ctggtggggg caagcgacaa acccgatcgt cccagctatc gcgtgatgaa atatctactc
    22261 gaccaggggt atcacgtcat tccggtttcg ccaaaagttg ctggcaaaac actgctgggc
    22321 cagcaggggt atggcacgct ggcagaagtt cctgaaaaag tcgatatggt cgatgttttc
    22381 cggaattcag aagcggcgtg gggcgtagcg caggaagcga tcgccatcgg cgcgaaaacc
    22441 ctctggatgc aactgggggt gattaatgag caagcggcag tgctggctcg ggatgccgga
    22501 ttaaatgtag tgatggatcg ctgcccggct attgagatcc ctcgcctggg cctggccaaa
    22561 taaaaaatcc ccggaaggca aaaaccttcc ggggatttgt tcagggatta gttacgcaga
    22621 cgcggggcct ggagttgttt acggatggtc tgcgccagct catccatgga tggctgttca
    22681 ggatgttcat cctgcaattc gctacttagc tgggcttcag ccaggtaggt atgaaccggt
    22741 aggccgttat cgtcctccat caccacgtga taccacggag cggcgcgaag ctcgtcattc
    22801 accgccagct catcaggcga cggttcagag agcgaataaa ccgggtcgat atccacgacc
    22861 actccgagat aacctaacag ggaatggcgg acctgctggc cgataccgaa tttgctggca
    22921 atcatagtca cctcccggga aatctttacc tttgatataa gggtaaaatt ccacatttca
    22981 agttacatga cgcgacaggc aaaccctttc agatatagcc cttccggata ggtagcgatc
    23041 accggatgat cggctgcctg acggaactgc tctataaatt gtacatcacg accggcatca
    23101 attgcggcat ccgcgatgat tttctgaaat aaatcgctgg tcatcagacc ggaacaggag
    23161 aaagtcagga gaataccgcc ttcattcagc agctgaatcg ccagcatgtt gatgtcttta
    23221 taaccacgac acgcgcccat caactggctt ttattctcaa caaacttcgg cgggtccatc
    23281 acgataacgt caaatttttc accgcgatcg cgataagtac gcagcaattt aaagacatca
    23341 tcacggacaa actcagcctt gctcagatcc agtttgttca gctcaacgtt ctgccgtgca
    23401 atatccaggg cttcctggga ggtatcaacg ctgacaacct ggctgcaacc gcccatcagt
    23461 gccgatacgg cgaaaccacc ggtataggag aaacagttaa gtacgcgttt attttcgacg
    23521 tagcggcggg tagccaggcg gctgtcacgc tgatccaggt agtagcccgt tttgtgtccg
    23581 tgctgaatat tcaccagcag tttcatgccg tgttcttcaa tcggcagcag ggcaggcggc
    23641 aactcgccgg tgatggggcc ctgagtcagc tccatccctt cttttttacg taccgcgacg
    23701 tcgctgcgat cgtaaatggc acattccggg tacagcgttt gcagggcgct aattaatgcc
    23761 gcgcgctgat actctgcgcc agcactcagc agttgcagca ccagaaaatt accgaaacga
    23821 tcgatagtaa tacccggcag gccatcagat tctccggcga ttaaacgata gctgtcgagg
    23881 ccatcttttt gcgccagcca gtcacgccat ttttgtgctt gctgcaaacg gcgggtaaaa
    23941 aaagcaatgt cgatagattc attcgggtca aacgtccaga cgcgcgcccg gatttgcgaa
    24001 gctggcgaat aagcgccgcg tgctaaccat tttccctgat gatcaacaat atcgatggtt
    24061 tcaccgaggc tggctttacc ttccatgcgg gcaacggccc cggaaaagac ccacggatgg
    24121 cgacgaagta atgatttttc gcgccctttg gctaacacta aacgtacact cataatttac
    24181 ttttctgtcg atgcaaagaa atgggcgtca ttgtccggag ttttccatgg atttgcaacg
    24241 cgtccattaa ggataaggag agcagcaatg tcgaaagtct gcataattgc ctgggtttac
    24301 ggtcgagttc agggcgttgg atttcgctat accacgcagt acgaagcgaa aaaactggga
    24361 ttagccgggt acgccaaaaa tcttgacgat ggcagcgtgg aagtggttgc ctgcggtgaa
    24421 gaagggcagg tagaaaaatt aatgcagtgg ttaaagagtg gcggcccgcg ttctgcgcgt
    24481 gtagaacggg tgctcagcga gccgcatcat ccctcggggg aattaaccga ttttcgtatc
    24541 cattaaatac attttaccgg ttttggcagg ccagcaattt tggtggcttg cttagccgga
    24601 cctttcggaa acagtcggta taaatagcgg ctgtttcctt tttcttcgcc aaatttattc
    24661 gccatcgctt ttaccagcat acgaatcgcc ggagaagtat tgaattccag atagaaatta
    24721 cgcacaaaac gcaccacttc ccagtgttct ggcgacagcg taatcccttc gttctctgca
    24781 atcaccactg ccagcggctc actccactgg ctgctttctt tgagatagcc ttcggtatcc
    24841 gtttctatct ctttaccttc gaagatcagc ataattatta ctaccttaat cagactggcg
    24901 gcagtgtaac aaaaaacaaa gccccgcata aagcgaggct atgaaagtgt tagcgggtaa
    24961 gattaatcgc ggctggcgaa gcccagaatg ctcagcaggc tgacgaagat gttgtacagc
    25021 gaaacataca ggctaaccgt ggcacgaata tagttcgttt caccgccatg aatgatgttg
    25081 ctggtttcaa acaagatagc gccagaggag atcagaatga agaccgcgct gatcgccaga
    25141 tgcagagcag gcagttgcag gaagatatta gcaaccatac caatcagcac caccacaata
    25201 cccgccatca gcataccgcc gaggaacgac atatctttgc gggtggtcag cacatatgcg
    25261 gagcagcaga agaacactaa cgccgttccg cccagtgcca tagcgattac gtcacccatt
    25321 ccggcagaca gataggtatt cagaattggt ccgaggatat aacccagaaa accggtaaag
    25381 gcgaatgcgg agataatccc ggtcggctta ttcgccgttt tataggtcag gaacatcaaa
    25441 ccatacatac ccaccagcgt cagaatcaga cccggagatg gcagcatcag caccgtactg
    25501 gcagttgcgg taatcgccga aaaggccagc gtcaggctca gcagaaaata ggtattacgc
    25561 agcaccttat gggtgctaag cagcgatgta cggtcatgtg aagaactaac aatacgatcc
    25621 attgagtcac tctctatgac agatgtaatt aattaagcag cataatgata atgcgtaagg
    25681 gcacccagaa gttttaccca tctttacgca tttgatctgg aacaggttta acagcggatt
    25741 atcaggttat taagcaatga tataacgctc tgagaatttc gacagacaaa agaaaaaggg
    25801 gttagcattt agctaacccc ttatcttatt tggcggaagc gcagagattc gaactctgga
    25861 accctttcgg gtcgccggtt ttcaagaccg aagaaaaaca aataaaaatc agtgtaatac
    25921 gtgaagctgc tgaaaattta atcaaattta acaccaagaa aaatcaaggt gttaggaagt
    25981 tgctcatagc tagtatacca agtatttttg ccagaaaatt ttctgtattg cctacttgct
    26041 aaccacgagc ggcagatcca gtgttggtgt gatttttgtt ttacgatcgt aaatcaacac
    26101 ctgattttct gttttatgcc cgctgaaaat ttgtttgtcg cgactgctgc cttcgtaatc
    26161 agagatcccc ttggctttta tatcgtgaaa attgcacccg aacggaacgc caactttttg
    26221 ctcggctgag cgtttagcct gattccacca gttgttcagc gttttagcta tgactttccc
    26281 gcctttggtt gtattgatca catattcgca tgtacaggaa gatacatttc gggctaactg
    26341 gatcgctgta cgtaatcgcg gtgaccattc cttgatttgt ttggtgccgg ttttgttttg
    26401 ctcaatgtag atccctttat ccataatatc ctgccatttc aactcaagca catcaccgag
    26461 ccttgccgca cagagatagg aaatctccat tgcaatgcgt aactgtggaa ttgcttccgc
    26521 atatatcgct gcatactgtt catcggtgat gtaaacagtg cgggctttaa gagagaattt
    26581 tctgactcct ttgcatgggt tattcttcac gtatccacgc tcatatcccc acccgtatac
    26641 tcgactcagg cttgccagtt catgatttgc ctgggtcttg ctctcaagcc ctcgtttatc
    26701 catgaaaatt cttacctgct cagttttgac attatcagca agcacttttc cgaataccat
    26761 cagcaacgcc ttctgatgtt gtcgataatc tttttgggtt cggggggcca gttctgtaaa
    26821 tgcaggggag tccataaaca tgtgccataa tttagctacg gtcattatgt tgtggagttt
    26881 tgctttttcc agttcataat tttgccagac tttagctacg ctggttttcc gcactcttcc
    26941 tagccctata gttcttgtac ttccttctgg tttccacacg taactgtaac cattcgatct
    27001 aacccgcggt ggcagtacat tatctttttt attttttctt ggtcttccca ttgttcagcg
    27061 cctcaaaatc gggttcagca gaaaccagtt cagatgtttt tggcatcgtt gtcagtccgt
    27121 gtggaatatc cctgcgtaga actattggtt cgtttttagg accgattaca aatgggatgc
    27181 cgtgcagtct taactggtgt tgctgttttg tgtatcgctc gtatttcgtg atctctttaa
    27241 tctctgctgg agatagagtt aattcgtaca tgtggtcacg tgcctttaca gcatgaccgc
    27301 cgccactata gctggtgggc ggcgatatgg gttgaacatt aaaaatcagc ctgactcggg
    27361 atcagttttt gccagatagc tgaaacgtat tttgcctgat aacgggcgtc atcaagtgca
    27421 ttatggcgct caccttcgaa tggaatagcc gttctggcat cgaagtctat ggctttcccc
    27481 agctcaacga ttgtgcgtac atcgcgatcg ttgtagtaac gccacgggca ggggatcccc
    27541 tgccgttcgt atgaacggcg caaaatcgtg ttgtcgaagt tggctccatt tccccagacc
    27601 tgaacaaaaa attcaccgga gttttcgtcg ataaattccc gcagttgtaa cagtgcatca
    27661 tctaacggga tttcatcggt cataatggca gattgcgctt cgcgtgattg cttaagccac
    27721 catttaatgg tgtcccgatc aatgactccg ccagcagttt ccagatcgat agtcttacta
    27781 aattccggtc ccatatctcc ggtttgcgga tcgaaaaata ttgcacctat tgagatgatc
    27841 ggggcattag gattttttcc catggtttca aggtcgatca ttagatggtc acacgtcctg
    27901 ctggtggatg tgatttcgtg atgaccgttc accgcaatta agggatctgc cgcttcgcca
    27961 gtttcactat cgctggtgtg atgctgatcg ttaccagcat tctcattgtt cggatgttca
    28021 gcgccttcta tttcctccgg atcatcttcc tgaacttcag cctgattttt ttcatcgaat
    28081 gtttcctggt atgttgcgtc gcccatcacc gcgccacagt caggacagtt gccgccaccg
    28141 ctctgaccgc aggcggtgca gactttttcc tcttcctgtt gcgctactgg ttcggattgt
    28201 ttcgtttctg gctcgtcttg taacgcattt tggctgtttt gttctgcttt ctggtcgttc
    28261 tgttccgttt ctagctggtt ctggttcaca gaatcgcagg tttcaatccc cttaacccat
    28321 ttcgaatcat tcgggtcgct aatcccttca acaaactcac cacgagaagc ggcaagcaat
    28381 ttgtcggcat ctggtcgact gatgtctgct gcctccataa ttttatttac ttcttcagcg
    28441 gtaactttta cctgtgactt gtcatccagc ggctgcgggt cctgatgatg ttcaactgta
    28501 tccgctgcca ttgtttcagc cgttgcctgt tcatctgcca ttgcgccaga tggttgcggg
    28561 ttttcttcat catttttttc ttcttctgtt ttgcactcag cagccagtcc gtcgttaatt
    28621 tcttccagga tatctttttc cggtgtatgc cgggcagcca tgagcgtttc agctgtggga
    28681 ttctcgtgat cagtttctgt caggttggca ttgatatacc cctgaaggcg tcccgggtag
    28741 tgataaaact cagggtgggc gctccggata agtgcaaaaa tagctgcgcg ggaatagtcc
    28801 aggataccag gggttgcgcg aagtgctgcg gaccattccc tgaacggact ttcttttttc
    28861 aggacgattt cttttgcgcg acgataaacg ctcacgggta tttcataaat attaaaatcc
    28921 atcggaagcg tggctgctgc agcctccaca tccagagtat cgagggtgtg tactaagtca
    28981 ggattacgat cggttttatt tccgccaccg gcatttgcac cggaagccgt gcgggtgatg
    29041 cgagaaacac gatttccttt catccactct tttgtcagca gacctcgatc ggtatagtca
    29101 gcgtccaggt atgcttcgaa aaaagcagtc attaacccca gactggaatt aacgggatta
    29161 gggaaaactt tgtctgtgtc gcgtaccagt ttatgaaggt cgcgaatctc cagcgggtcg
    29221 agcagctttg tgttgtggga aatagccagg gcagtaacag ccggcagttc ttcagctcgt
    29281 gcgatatgta atgcctgaag ttcttcccgt gaaacgtgcg ttaccggttt ttcgctgcca
    29341 tgttgcgcaa gccagcgaat gggtagttcc tgaccggaaa ttggcaggag catattatcc
    29401 tcaatctcag ccatgtcttc gccattgacg ttggtattgt cagtgctggc tggtttttcc
    29461 tgaactgagg gggaaggcgc tataaatatc attgtgatgc catcttcccc gcctttttca
    29521 tagcggttgc agaattcagt atcaaacacg ccttccggtg gaaggtcatt cacaacgggg
    29581 aaatggacgc ggacgggttt tttgaaatca tcagcgtcgt agcctgcctc gtccattgca
    29641 acaacgcacc gcgagattgc tacagataat ttcttttctg tactccagta aaaaccccct
    29701 ttaatcccaa agcgttttct gaccttgtca ttttttgctt cgcaatatag cgcaaattct
    29761 tctttatcag tgctcattga taaacctcat aactatttta aggttgtacg aacccctgcc
    29821 attgctggca tgcttaatca acgggtatgg cgttaatatg gctggtggat tatccagccg
    29881 gtatttcgtt attcaggtac agcgatactt tttttaacgg gaggcattcg ccagaaattt
    29941 tttgctcgtc tcttgcccgg aggcatgatt ctttactggc ataaattccg gtaatcacat
    30001 tctgtggctc acccgttata agaaaaacag tcattaccag tgcaaatgca gaagtcactg
    30061 ctgccccccg aaaataccaa gtttaagaag ggcaattctg gagagtatgg aattatcatt
    30121 gagaagataa ggctcatatt ttctcatctt aatggcatct tcagtaaact cccggttact
    30181 gagcagaaca ccaatatcaa aacacccttc agacgtatta acgtttggta ataacgtttc
    30241 cattatcgtg tcctcaacaa tgaattttgt gatgtggtgc ctggtgcctc caggtgacgt
    30301 taaccagtta acaattaacg ccggaataca gaaggatgcc cgttacgccc cgtaaaagac
    30361 cactttacgg atttaactgt tccgcgtgcg catagccgca ttcaccgcat cacaaaattc
    30421 actttaaaaa ggatggcaga gcagtcatgg agtcgaactg atgccaccaa agactacaca
    30481 tggatattgt ggcggggctg tcacttaagc gtatggtcag cctgacaacc cggtgtcctc
    30541 aaggggaagg aataaccccg ccatacttac cgccgcgcca tttcgcggat tgccacaacc
    30601 ggaagcgcac ggtcgaatta aatttaacga caaagtacag agagacgagc ttctccgtgc
    30661 gctttcgtgt tatgccctga cttttcaggg taaattaacc agtggcaacc tgtttttact
    30721 ggtgctaatc agttagtgtt tctggctaac cagcgacgcg cgccagcttc ggttttaaac
    30781 tttttacttt tggtatacgt catcgcggta aacgtgccgt cctggttagg gaacacaccg
    30841 cataccagag attcgttgtt gccaagattg agcgtatcca tgttgacctc atttcccctt
    30901 aacgccgggg tagcggaaca aaaaacctgc tgcatagtta aacgtaatcc ccgccgtcat
    30961 gttcatacgc ctcaggctgg ctacttaacc cctgaccact gccgggtaac tcgaagtatt
    31021 gccctgcgtt ctgtgggtcg gggtgggtgt atggatacaa tttacaaatt aaaatttaac
    31081 tggtcaacat gtttattatt aaatttgtaa ttgcgagctg ttgcattagt cccaaaatgg
    31141 gactactata cagttatgaa gattatctca gttaaaacac tcagggattt ttgggcggag
    31201 aatcctgatg cagaacaacc gctaagggca tgggtggatg aggcggcaaa agctgactgg
    31261 aaaagcccag cagacattaa ggcacaatac cgaacggcca gtatattgaa aaaccggcgc
    31321 gtggtattca acgtaaaagg taatcattac cgtttaattg ttgcaattgc gtatcagcga
    31381 ggatgggtat ttgttaaatt catcggaaac cacaaggaat acgatgctat tgatgctgat
    31441 accatcgaac tggagtaagc atgaacatca aacctattcg tacagagcaa gattatgaag
    31501 ccgcgttgcg tgctgttgaa cccatgttcg acaatgagcc cgaaattgac acgcctgagg
    31561 gggatttttt tgaggtgatg tgtttgctaa tagaggaata tgaaaaaaaa cattatccca
    31621 ttgagccacc atccccaatt gaagctataa gatttcgcat ggaacagcag gggctgactg
    31681 tgaaagattt ggaacccgca attgggaaaa aaaatcgggt ttatgaggtg ttgaatggta
    31741 ccagaagcct tacgttacca atgattcgcc gtcttcataa tcaatttggt atccccctgg
    31801 aaagcctggt tggattataa aatctgctag tcatttgcct gatgctcgtt ccagaaaagg
    31861 aatgcatcag gcagttttgt ttttctgccg cagtaactct tcaagtttcc gtttatagaa
    31921 atcgcgtttt tgctccatat cacgaatgat ctgctctgcg tcgctttgag gtaactcatc
    31981 taaaagcgat atgatttttc gttgttgttc tgtaagttgc ggttggttgt cattactgga
    32041 tacagccatt ttatcgccga gagtttcttc ttccataaag aaccaatgga cgggatgttg
    32101 tgagagctct gctaattttt ccagtttatc cattcttggc atcacgcctt tcaaccaacc
    32161 ttgcacggat tggggtttta caccaagacg tcttcccagc tctgactggt ttatattcaa
    32221 ttcctgcaac acctgctgaa ggcgttttac aaagatcatc accacccctc gtaaactagt
    32281 tccgcgatcc tacagaaaaa attgataagt ggcattacaa atagaagttg aaatttaaat
    32341 ttaaatttgt aatcatcggt gtcatcgtaa agttcggagg gaaacatgca aaaaagtact
    32401 caagtgaaaa tcctgtcaat aatgagccaa tcagaattag ggcgtcgtct tggtaaaaca
    32461 ccgcaaacca taagtgggtg gtttaaaaaa cgagtgccag cggaggaggt tataccagca
    32521 tgtgaggcgc ttgactgggg agtaactccg catgaattgc gccctgataa ataccctaat
    32581 ccaaccgatg gtttacctgt tgagtatcag gctaacgcac aagcagcggc gggagttgat
    32641 tcatgaaaat caagcatgag cacatccgca tggcgatgaa tgcgtggctg ctttatccga
    32701 gggtagggcg caaaaaaatc gctgatgata tagcgacagc atattttgag cttgaaatga
    32761 cttatccacc aatgcatgac acctctacga cagagggtat tggattgaat atacaaaata
    32821 tttttcgctg gcttgaaaag gatacgcctg atgctgttga aaaaattcag gcactaattc
    32881 cggctatcct gactgttctt ccgcgtgaac tgcgttatca cctcagtatt tttgacactg
    32941 ttgagcgccg tgcattactg gcggcgcagg aagcgttgag cacggcaatt gatgcacatg
    33001 atgatgctgt ccaggccgtt taccggaaag cgtatttcag cgacggcggg tcatccggcg
    33061 agtctgttgt ggtgcattga tatttatgcc agccccctgc tgattctgtt gattggggaa
    33121 tcacagaata tcaccagagg atggttcgtc acaagatgag gcaattatgg ccgcattacc
    33181 atacatgcaa ctgtacatag ctgattacct ggctgacacc atgcatttgt ccgcagagga
    33241 gcacggtgcg tatttgttgc tgatgttcaa ttactggcaa acagggaaac caatacctaa
    33301 aaacaggctg gcaaaaattt cccgtctgac taacgagcga tgggctgatg tggaaccatc
    33361 cttgcgggag tttttttgcg ataacggcga tgaatgggtg catcttcgga ttgaggaaga
    33421 tctggcatca gtcagggaaa aattgaccaa aaaatcagcc gccggaaaag catctgttca
    33481 ggccagaaga agcagaaagg aagtatatgt tcaaacaaaa caagagagag atttaacagg
    33541 tgttcaaaca aatgttggtg ttgtgtttga acatgacgca aacacaaaag caactaataa
    33601 agatacagat ctaaaagaat taaaccccac acataacgtg cgtgaacgcg agagtattcc
    33661 gaccagtgag ttgcatggtg cgccgttgca gacagccgaa cctgaatacc tggacggcct
    33721 gagcgaaccg atcgggaaat tttcgatgac cactgtctgg cagccgtcgc cggattttcg
    33781 acaacgggca gcagtgtggg gtatggctct gcctgagccg gaatttacac ctgctgagct
    33841 tgccgcattc cgggattact ggatggcgga ggggaaggtt ttcacacagg ttcagtggga
    33901 gcagaaattt gcccgccacg tgcagcacgt cagggcacag gtaaaaccag tcagcaaggg
    33961 ggtaagccat gcagcatcag gtggcacggc atcacgggca attcaggaaa tccgggcagc
    34021 acgcgaacag tgggaacgag acaacggatt tatcagcaac ggaaacggcc tggaagctgt
    34081 gggagcttat gggggaggtg tattcgaacc gctggactca gaagaacggg gccgcacctt
    34141 cgaagctctg gattgcccag attggcgcga tgactgaaca gcaaatccgg ctggtctgcc
    34201 gtcagtgcat ggaccgctgc cgggcgggtg aaacgtggcc cccggacctg gctgagtttg
    34261 ttgcgctgat ttcggggagt ggggcaaatc catttggtct tacggtggat gccgtgatgg
    34321 aagagtaccg gcgctggcgc aatgaatcct ggcgatacga cgggagtgat aaatacccgt
    34381 ggccacagcc tgtgctgtac cacatctgcc tcgaaatgcg taccagaggg attgagcgcc
    34441 agatgacgca gggtgagtta aaacgacttg cggaacggca actgacgaaa tgggcaaagc
    34501 atgttggtaa cgggatgagt gttccgccag tgcgacgaca actggaaggg gcgaaacacc
    34561 cgcaagggcc aacgccaatt gaacggctga aacaggaata cgaacgccgg aaggcagctg
    34621 gttttatttg aatctgagaa acgattttgt cggaggaaat tttaatggaa accgtatttg
    34681 acgcactgaa agcactgaaa aaagcctctt cacaggtagt ggcatcgcgc cttggaatca
    34741 gccgcgaaga tgcggtcaac gaactgtgga aactgaagcg ccgcggtgaa gcggataaca
    34801 agggttcgat gtggtggctg attcaggctg gtgaaagtga accagtgtca ccggtaccga
    34861 aagtgacagc gcaaatgctg attaaggcga ttgaacaaca tggcccacaa acggcggatg
    34921 agctggcact gatgttcggg attacctccc gccgggcgaa ttcatcgctg gccatggcaa
    34981 tcagcaaagg gcgtctgatt cgcgtgaatc agggcggtaa atttcgttac tgcataccgg
    35041 tcgctgattt accggcagag ccggaagctg catccgtagc ggaaacggat ggtaaagcct
    35101 ttcctcagcc agcaggtgtt gcgttaccag tccgggaagc ggaaacacag gaagatatta
    35161 aaacagaaac tgtggctgat attgtgcaat cgttgccatc gtttactgaa acgcgagcgg
    35221 atgacctggt tttaccatcg ctgcatgtgg ctaaccgcga gctgcgccgg gcaaaaggtc
    35281 aggttcagaa gtgggagcga gtctgtgccg cgctgcggga actgaacaaa cacagggata
    35341 ttctccggga tattaccgcc accagagagc tgctgcggtg agtggctgga agaagtggcg
    35401 ctgggcggaa attatgatac tccggcagtg tgcgggaacg atgacagtcg aaagcatcgg
    35461 cagtctgatt ggccgtagtg gggctgccgt caggacgaaa gcgcgggaac tgggtatctg
    35521 catgatgtta cggggtgatt atcaccagtc agccaaatgt tcacagcgtg atattgagct
    35581 ggcgcggcaa ctgcatcagc gtggcgtacc ccgacgggaa attgccgaaa agtttgggat
    35641 gaagttgggc gcagtgaata actacgttta tttcgacagg agggttcagg cgtgagggtg
    35701 agaatttata tcgccggtcc gatgacggga tatgaaaatt tcaaccgtga ggcgtttcac
    35761 aaggcggaag aagcactgaa acaggaaggg cataccgtat taaatcccgc agtacttccg
    35821 gacgggctga ctcagccaca ttacatggat atttgtatgg caatgattcg ttgtgtggat
    35881 gcggtttata tgctgaaagg ctggcagcgg tcggcaggcg ctaaggcaga actggcactg
    35941 gcggagaaac tggggcatgc agttattttc caggaggcaa ccagtgagaa aaattaatta
    36001 tcagacgcta cgtgagatag caaaactggc aacccagggc gaatgggtcg cattcatttc
    36061 gccgggcact gatacatatg cggtgcatac gccgggggat gaacgttgcg gagacattat
    36121 caaatggcct ggctttgatg accagaaaaa tgcagagaac aacgcagaat ttattgcagc
    36181 tttcaaccct gaagtagtac aggcactgct ggatgaacgg gaagcccaaa gcaaacgtat
    36241 tgccgagctg gaagctaatc ttgtggcgct ggctgcggag aatgcacttg ctcgtaaagc
    36301 agttcaggca ttctgcgatg ttgttggcga caacaccgag gttatttgcg aggaggttgg
    36361 gagagatggc gttctggtta ttttggaggc catgaaggca acaggaaata tgccagccac
    36421 cgatgctttc ctggctgatg tacgagcgga ggtgtttaac gacctttgcg cggcgtttgt
    36481 caggcacgca aaagttgcag gactggatga tgccgatacc gtaacgttta aggaagcgac
    36541 agaagcattg ctgcattgtg cagaacaact tcgcgcacct gaataattaa atttagcact
    36601 gtaaataaaa tttaatcctt aaccggaggg attcctgcac cctcagaaca tcaggagacc
    36661 gcccggcagg gcggtagtga aatgcgaaag ttcaaaataa ttattgaaac gggaatagcc
    36721 ggtggagatt ttgaggatgt attcgaagtg gacgatgacg caacacctga tgaaattcat
    36781 gacgaagcaa aagaaatttt ctttaactac tgcaattact catatcacga aataaaagac
    36841 gaagaggaag aacaaaatgg ctgattttgg ttcaactaaa tataacgcca gttttgaaga
    36901 atggcatgaa ctgttaatgg actatgcaga gttacgcggt ggaagtgccg ccgatgctga
    36961 agcatggcgt gatgactatg aagcaggaaa aactccggtt gaagcttatt gtgatgagtg
    37021 gggtgaggac gatgaagatt aactatcagg cgatggtgca ggccaggcga ttcgataccg
    37081 gctcgtgttc agagtcatgt gaaatctggg gtgaacaatt ggaattgaaa ttcgaagagg
    37141 tggtggcatg acaatcgtaa aaacccatac cggcatcgtg atcaccaaag acggtccgca
    37201 ggtaaaaaaa ctgcaccaga cggagcggat gtgggtcgtc gacaaaaacg agttttacca
    37261 caaagaaacc ggacgccgcc actttgcaga aaatacgcgc cgccgactac tgatcgacac
    37321 catcaagcct gtcgaggtga agcatgttta aacagaacga aaaatctatc gctcaaattg
    37381 ctgagtatat cccgcgtgcg tgccggggta tgcagttgca ggaagccaaa gcgcgcctgg
    37441 agaaaaaaat tgcgctctat atcaatgacg gctgtgatgc tgccgttctt aacgcggcgt
    37501 tcgcaccagc tcttaacagt catacgcggg agtctttttt ttcgtgcatc gcagcgcaga
    37561 tccacaaagg aggcaaccag tgagcaagat tgactatcag gcattgcgtg aggcagcaca
    37621 aaactatcgg tcgatgctgg cgtggtatca ggagaaacca gacagtccaa acgctgagca
    37681 ggattgtgat gcagctttgt ctgcgtttaa gcgagaaatc cgtcatcgtg aagtggatat
    37741 tatcgctgat ttgctggatg aactggagga agcaaaacag cgtatcgacg agcaggagtc
    37801 ccgcacagtg aagttaccag aaccattcaa gctggctaaa tcatcaagtg gattaacgta
    37861 ctactacgct gatgaggtca atgcagcact gactgctgct ggtattcgca tagaaggaga
    37921 gtgagatgac cactattacc aaagagcgac tgcaatggct ggctaacatt tctggccgcg
    37981 atgacattga cgatatagac ggcggtgaaa ttcgtgagct ggcgcttatc gcactggcat
    38041 cgctggaagc agaaccggtg gtttacatgt gggatagcga aagaaaagat attgatgctc
    38101 ctgggtatta tcggactgag catttagtgt tcgctgagag tagtgttaag caatggggag
    38161 ggcgagttgt tccactttac accgccccgc cagcgccgat agtgccagct gttctggaac
    38221 gtttgcgagc tattgtagcg gacccacgcg cattacctcg cagaaaagaa tgggttagcg
    38281 ggcagcagta cagttacgta cttctcgaaa acgtagaagc tatggttgat gaagcctgcc
    38341 gcgcagccat gcttcaggga gataaataat gattaatcga accaaactgg agcacattct
    38401 cgaatatgcc aggcagcaga aatgtattgg gcaactttgt aaaattccac caggggatat
    38461 ggttgaaatc atggagattg ccatgcgcaa ggctggcaac tctccgataa ctcaggctag
    38521 tctgcctggc ggtttcacca ttgaggaggc gaaggagtta catgaagacc tggtgcgcag
    38581 ccacataagc aaggctttaa gtggcgaaaa gatgaagaag aaggatctcg atgctgattt
    38641 gtgctggatc catggggtta tagtccaggc atcctggttt gtaaaagcat cactggagca
    38701 gaatgcgcta acgggtaact ctcaggtaat ccctgatgac ggtcgcgaac agtttgaaac
    38761 acttatcagg ttccatgcag atgataaaaa ccatgaaaca ttattgcttc gtgctaacga
    38821 agggatgaat taccgggatc caaatgtgga tttggcgtgg atattctgga aatccagtcg
    38881 agagcacata ttgccgggaa aaagcgacaa taatccagca ttaggcgatc aggtgagtga
    38941 attaactatg ttggtcaagc gattagccag ttcattaaaa agcgtcaata aatcaagcaa
    39001 actaccggat aaagcgatgg actacctgaa gcgaaacgga ctgataagcg cggaggatat
    39061 tttacgatga cctggcctga ggcattcgtg gcggtaggaa ttgcaatggc ggtggcgctg
    39121 gtgctggtgg tgtattcgat ttgccgctgg ggataaaaac ggtttgcggg gaaagaagag
    39181 ttaagtagaa gtgctgcggg tgcttgaggc tgtctgcctc gggcatgccg ccgtaaggca
    39241 gacagagaaa agccccagtt aacattacgc gtcttgcagg acgcttaaca ttaatctgag
    39301 gcccaatcta tgcttcacaa acgtaggtta gcctcttacg gaccgaaagg tcaaggagaa
    39361 gcagactatg aagcagcaaa aggcgatgtt aatcgccctg atcgtcatct gtttaaccgt
    39421 catagtgacg gcactggtaa cgaggaaaga cctctgcgag gtacgaatcc gaaccggcca
    39481 gacggaggtt gctgtcttcg tagactacga atctgagaag taagagtgac caggcgaggg
    39541 agaaatccct cgccacctct gatgtgtcag gcatcctcaa cgcacctgag ataaaatcta
    39601 tttggctgtt tctgggttcg aactgatgat tatttggtgc taatacatga tttaattccc
    39661 tgaatcctct tgcttttccc attggtgggg tctaagactc ctcgaaggga ataaaaatca
    39721 atgatagtag aaccatctgc caacttataa atgtcggcat tatctccaac cgctaatacg
    39781 ctgtaatact tgccaatttt ttgagacgtg atagtggcta agagaggtgt acgctgccac
    39841 tcactgagaa tgcattcttg tatttcttgt ggtggagaat ttgttttaaa cgagataata
    39901 acattttgtt cttctctgta gttataaggg ttgacgcatc ctgaaattgc aaaaaccaat
    39961 agaaaacaaa ttgttttatt catttagaag cccttttata agtatttctt ggttatttaa
    40021 gttttttgtt agtgaaaata taaactataa aaagtcagat tcataacttt gccctccatg
    40081 gtaaacgcca gcgataaaga ctttattgcc atcaacggca aaagcaatga tcgttctgtg
    40141 gcggaagtga gttacccgca ttccctgtcg aatatcatcg cgtttattgc cccgatgcgg
    40201 gaatgtggaa aaaccatcaa gataatcaag aagcgcgttg gcataattgt cagcaatggc
    40261 gctccctgcc ttctctgtta tatatctgtg caggttgatt atctgctgtt cggcctcaga
    40321 agtaatgatg acttcatacg tcatgcagat tacttcccgg ctcgaatcgc ggcgcgaacc
    40381 tgtgaaatgg agcgtccgtt gtttgggttt tcgcggatag aatcgagaga tggggcggct
    40441 gaatgcgtta accacgcttc gattgcttta tcgcgctcat tcagtgcgcg aagcccttca
    40501 cgaatgacct cactttctga agcataggca ccggaagcca cacgggcgcg caccatgtca
    40561 gccatctcgt tagttaatgt aatgctgaat tgttgggttg tacgcatggt aaacctcacg
    40621 gagtaggata gaacactatt cgatgatagc acactgcctg ttgacgacac cagaaatcag
    40681 atacaatatt gccgcacgcc agcctgaaca actggcacct gctgcgccag cagagacaac
    40741 cgatggcgca cgataccaga ttttacaatt cggataactc cgccgcccct gccagcaggc
    40801 acgggcggcg ttctcatgca ttcaaatctg actggttaca gcatccccca tgcactgaag
    40861 aacaggccga atggctgatc cagtgttacc gcaggcgcgg atatgagttt gagaaagccc
    40921 tcagcttcga tcgtcgtcac tggataatct ccgtcaggct cccttattcc gaacgcccac
    40981 cgcgtccgtc ccgcacattc cagcagcgca tctggaggta acgtgcgggt attacttcga
    41041 cctgttctgg taccggaact cgggatggtg ctccttaagc cgggccgtga atcaatgtca
    41101 gcattccata acggcagaat attggtggaa ccggaaccga aaaacatgcg cggtctgccg
    41161 tccggagtcg ttcctgccgt tcgacagccg ctggcagagg ataaaacatt actgccattt
    41221 ttcagcaacg aacgggtgat tcgtgctgcg ggtggcgctg gtgcactgtc tgactggtta
    41281 ttacgtcacg tgaaatcctg ccagtggcca cacggtgatt atcatcacag cgaaaccgtc
    41341 attcaccgtt acggtaccgg cgcgatggtg ttgtgctggc actgtgacaa ccagctgcgc
    41401 gaccagacat cagaatcact cgatcaactt gctcagcaga atctggcagc ctggatgatt
    41461 gatgtcatcc gtcacgcaat gaatgacaca caggagcggg aactgtcgct ggcagagtta
    41521 tgctggtggg ctgtttgtca tcaggtggct gatgcgcttc cggagtctgt attgcgtcgt
    41581 tcgctgggat taccagcgga aaaaatccgc tccgtatacc gtgagagtga catcgtaccg
    41641 ggagaacaga cagccatcag catactgaag cagcgcacaa aaaatattgc gctgccactt
    41701 cacgtccacc agcaacaaaa tccaccacag aaaaaaacgg ttgtcagtat cgctgttgat
    41761 ccggagtctc ctgaatcgtt catgaggcgg cctaaacgtc gccgttgggt taatgagaaa
    41821 tacacgcgct gggtaaagac acagccgtgt gcgtgttgtg gtaagcctgc tgacgatccg
    41881 catcacctga ttggtcatgg tcagggggga atggggacaa aggcccacga tattttcacg
    41941 ctaccgttgt gccgggagca ccacaacgaa cttcatgcag acccgctgaa gtttgagaaa
    42001 aagtacggct ctcagattga gttaattttt cgttttcttg atcacgcctt tgcgactggc
    42061 gtgctcgggt aaaagaggtg actgatgctc atagatttgg ttttacctta cccgccgacg
    42121 gtgaacactt actggcgacg ccgtggcagc acatattttg tatcaaaatc cggggagcgt
    42181 tatcgccggg cagtggcact tattgttcgc cagcagcagt tgaaattaag cctgtccgga
    42241 cggctggcaa taaaaattat tgcagagcca ccggataagc gtcgtcgtga cctggacaat
    42301 attctgaaag caccgctgga tgcgctgacg cacgcggggt tgttaatgga cgatgagcag
    42361 tttgatgaaa tcaatattgt acgtggccag ccagtatctg gtggacgtct gggggtgaag
    42421 atttacccca taatgcttga agggcaggtc aaaaaatgaa actggaagat ttaccgaaat
    42481 actactcccc aaaatccccc ggcctgactg atgcatcggc ctcaacgtcg aaagatgcgc
    42541 tgagtatcac tgatgtgatg gccgcgcagg gcatgacaca gaatcgggct gagatggggt
    42601 tttctgcgtt ccttgggaaa atgggcatta gtatgaatga cagagagcgg gcaacagaat
    42661 tgctgacaga atatgcactc agtcggtgtg atcgcgtggc ggcgttaaga aaactcccgg
    42721 cagaaataaa accggcagtg atgcgtatta tggcttcgta tgcgtttgaa gattatgccc
    42781 gtagcgcggc gagcaaaaaa cagtgcccct gctgtcacgg aaaaaaattt attgaaagcg
    42841 aggtttttac aaacaagatc cagtatccgg atggtaagcc gccagtgtgg gcaaagtgca
    42901 caaaaggcgt gtatccgtct tactgggagg aatggaaaaa agtcagggag gtggtaaaag
    42961 ttgcctgtcc ggagtgtgga gggaagggag aggtatccac tgcctgtaaa gattgtcgtg
    43021 ggcgcggtgt tgccattcat cgtgaagagt cggaaaaaca gggtgtgccg gttttcagaa
    43081 actgtcagcg ttgtggtggg cgtggctatg aaagattacc ttcaacggag gcatttaatg
    43141 ccatatgtaa tgtaaccgat gccatatctc tcgatacatg gaaaaaaaca gttaaacgtt
    43201 tttacgatac gctggtggtg cagtttgata ttgaagaagc atgggcagaa caacaactga
    43261 aaaaggtgac cagatagctt tgttgatttt tcccgaatct gtggtaaatt tgccctaacg
    43321 atgggtgttt tatgcctgac gttagaagat tttttacacc ccgccgcctg gcgggttttt
    43381 ttatgactga aatcacgcca gtacagtaaa cgcgctggtg gcggtgaata ccggtctttc
    43441 agcttgctgg ctttttcgac aagagttatt ggtgtgtcac gttaaccgga aaagggaaaa
    43501 agacatgctg aaacagcagg atatgaccga aaccgccaga gtggtgttta atgaattaag
    43561 cgtcaccgaa ccggcgactg tcggggagat tgcacagaat acttaccttt cacgtgaacg
    43621 ctgccagtta atactgaccc agctggttat ggcggggctg gcagactatc agttcggttg
    43681 ttacagacgc cttcagtcct gaaggctttt ttatttgtgg taaatgggcg gctggtgggt
    43741 gttaggggca ctcaccagcc atctgctcat gcgtccggat cacaagcaaa cctcaggccc
    43801 actgctttgc gcaaaagcag aatgagccta tcagagacag gcttaatgat ccatgcttag
    43861 tactgtaaaa atatccagtt gtgagttaat caacgccgac tgcctggaat ttatccggtc
    43921 gttacccgaa aattctgttg acctgatagt cacggacccg ccgtacttta aagtgaagcc
    43981 tgagggctgg gataaccagt ggaagggcga cgatgattac ctgaagtggc tggaccagtg
    44041 tcttgcgcag ttctggcggg tgctgaaacc tgccggaagt ctttacctgt tctgtggcca
    44101 tcgcctggca tctgacattg aaatcatgat gcgtgaacgc ttcagtgtgc tgaaccatat
    44161 tatctgggcg aagccgtccg gacgctggaa cgggtgcaac aaggaaagcc tgagggcgta
    44221 tttccccgcc acagagcgca ttctgttcgc ggaacattat caggggccgt atcgtccgaa
    44281 agatgccggg tatgaggcga agggcagggc actgaaacag catgtgatgg ccccgctgat
    44341 tgcttacttt cgtgatgcac gtgctgccct ggggataacg gcaaaacaga ttgctgatgc
    44401 cacaggaaag aaaaacatgg tgtcgcactg gttcagtgcc agtcagtggc agctaccgga
    44461 cgaaagcgat tatctgaaat tacaggcgct gtttgcccgg gtggcagaag agaagcatca
    44521 gcgcggagaa ctggaaaagc cacatcacca actggtcagc acatacagtg aactgaaccg
    44581 acagtatacg gaactgcaga gtgaatataa gcatctgcgg cggtatttcg gtgtgacggc
    44641 gcaggtgccg tacaccgatg tgtggacgca taaaccggtg cagtactatc ccgggaaaca
    44701 tccgtgcgaa aaaccggcag aaatgctgca gcagataatc agcgcgagca gtcgtccggg
    44761 tgacttggtt gcagatttct tcatggggtc gggctcaacc gtcaaagcag cgctggcact
    44821 ggggcgtcgt gcaactggtg ttgagctgga gagtgaacgt tttttacaga cagtgaagga
    44881 gatccgggac gatttttgca ggtgacagag gttgaggaaa ggttgaaggg aggatcattt
    44941 gttattgttg tatataatta ttctacccct ttcctgagtc aggtaaatat ataatggcag
    45001 ttgtctgttc tgtaattctg gtttgttccc cgatcaatat ttttattgag aaagacaaac
    45061 tttcacttgt gccgggcatg gtcattctgg tcaccagaaa catcagggag ctttttcgca
    45121 cttacacaga ccgggttaag gttgctgata taaatcacga tatagtcagt caatatctgg
    45181 aaggtgggcg aaggcggatg agctccccac aggcatgttt tccgcattat ctcacttctg
    45241 gcattcatca gcctgtactg gcagaggcgt taattactaa tcatggattt cccagtgctc
    45301 cttctggtga tttgtccacg atgacagggc tttcatgtct ttctctgttt gccaacgatg
    45361 agagcctgtc atcatttttg tcaaggtgtt taagcaatac aagcgataaa gtcagggcaa
    45421 taattcagag cgatatatcc gtaaactgga agcttggtgt tattgcgtta caactgcata
    45481 tgagcgaaag cttgttaaag aaaaagctta aagatgaagg aaacagcttc agccgcctgt
    45541 tgctggaaga gaggatgcgg ggggcagtaa atatgttatg ctttcaatat ggacggggag
    45601 cgatagtggc agaaaaatgc ggttattcaa gcctgtctta ctttatatca gtattccaca
    45661 ggtattatgg agtacctcct gacaaatatg tgtctctgca tatggttgac ttttaatttc
    45721 atcctgctgg cggatggagt gctggtggtc ggcattgtgg tggtgctttt tcctttggtt
    45781 aaaaaggaca tgccaggtag ttagcgggta tcagtcatgc ccagaaaatt ttaaatgtct
    45841 cacaattcag acggttgaca gttgtctgtt ttgcggggag tttgttaaaa gaaactggca
    45901 tggtgaatcc ccctgagcgg aggggcgact ggtgcatcag gtgttatctt tgatacccag
    45961 gaaagtgaac gcgcgggttc ggtggcaccg ggctgaactc accgggaggc acccggcact
    46021 atgcgcatga tgtacagata cgcggcttca gcccctctcc ggaggggctt ttctgtgcct
    46081 tatgcatcac agtttctgga accctgagta ctacagtatc agtcagggtg ctatatttgc
    46141 agatgtgatg aaagcctgcc ggcaggcagg gcgtatcgga aatgacccag taagagaaaa
    46201 cgttgactca gataccggtg ctgagttacc gggaaaccgg catcacatga ccgctatcct
    46261 tcatggctct ctcaggagag cctttttact ggagaaaacc gcttccccgt aaaatgccgt
    46321 gttgctcaca attcagtcag ttgaaggttg tctgcccgac tgagaatttg ttaaaaaatt
    46381 tctgcatgat gaatccccct gtgcggaggg gcggctggtg tacggaatgg tctctgatga
    46441 tcgttagcga gaatgacgcg ggttcggtgg caccgggctg aactcaccgg gaggcacccg
    46501 gcatcatgta atgaacggta catagcgcta ctcttcagcc cctctccgga ggggctttct
    46561 tatggataaa aaaagcccgc atggtgagat gcgggcagca aggaataaac aacaaaacgt
    46621 gaagtaatca atttttcagc tggcgaataa tacccgacag taatcactct gcgcaactgc
    46681 gcggcctttt tcgtattgcg ggctgtagtc ttcctcctgc cattgtcctg taacttccgg
    46741 acttcagccc gcccctcatc tgactcacaa cattatcccg accgggagga ttcatggcat
    46801 ttaaacacta tgacgtggtc agggcggcgt cgccgtcaga ccttgcggag cgactgactc
    46861 aaaaactgaa ggaggggtgg cagccatttg gcagtcctgt cgccatcacg ccttataccc
    46921 tgatgcaggc cattgcggcg gaaggtgatg tcaccacacc tgtggtggtg aagccgtcgg
    46981 atggagaagg cacagttatc agcgccacca gcgacccgga gtattacttt gttgtggttc
    47041 tggcagggca gtcaaacggc atgtcgtatg gtgaaggcct tccgctgccg gagacatatg
    47101 accgtccgga cccgcgtatt aagcagctgg cgcgccgaag tacggtgaca ccggg
//
LOCUS       DAZGQL010000027        46705 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.027, whole genome shotgun sequence.
ACCESSION   DAZGQL010000027 DAZGQL010000000
VERSION     DAZGQL010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 46705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46705
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.027"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(36..350)
                     /gene="pspE"
                     /locus_tag="ACHKIR_001790"
     CDS             complement(36..350)
                     /gene="pspE"
                     /locus_tag="ACHKIR_001790"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HFO9989539.1"
                     /translation="MFKKGLLALTLVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(425..646)
                     /gene="pspD"
                     /locus_tag="ACHKIR_001791"
     CDS             complement(425..646)
                     /gene="pspD"
                     /locus_tag="ACHKIR_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HFO9989540.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(655..1014)
                     /gene="pspC"
                     /locus_tag="ACHKIR_001792"
     CDS             complement(655..1014)
                     /gene="pspC"
                     /locus_tag="ACHKIR_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HFO9989541.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(1014..1238)
                     /gene="pspB"
                     /locus_tag="ACHKIR_001793"
     CDS             complement(1014..1238)
                     /gene="pspB"
                     /locus_tag="ACHKIR_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009271 - phage shock [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HFO9989542.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(1292..1960)
                     /gene="pspA"
                     /locus_tag="ACHKIR_001794"
     CDS             complement(1292..1960)
                     /gene="pspA"
                     /locus_tag="ACHKIR_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HFO9989543.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            2127..3104
                     /gene="pspF"
                     /locus_tag="ACHKIR_001795"
     CDS             2127..3104
                     /gene="pspF"
                     /locus_tag="ACHKIR_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HFO9989544.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(3224..4489)
                     /gene="puuE"
                     /locus_tag="ACHKIR_001796"
     CDS             complement(3224..4489)
                     /gene="puuE"
                     /locus_tag="ACHKIR_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HFO9989545.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKMFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLTNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDEAMKILQDALSD"
     gene            complement(4527..5807)
                     /gene="puuB"
                     /locus_tag="ACHKIR_001797"
     CDS             complement(4527..5807)
                     /gene="puuB"
                     /locus_tag="ACHKIR_001797"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HFO9989546.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESISCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVHELSAVTQIQHTTPAVV
                     RTANGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(5809..7296)
                     /gene="puuC"
                     /locus_tag="ACHKIR_001798"
     CDS             complement(5809..7296)
                     /gene="puuC"
                     /locus_tag="ACHKIR_001798"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HFO9989547.1"
                     /translation="MNFHHLAYWQDKALSLAIETRLFINGEYTAAAENETFETVDPVT
                     QAPLANIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEANAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQKAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDCAHADSVHSFIREGESKGQLLLDGRNAELAAAIGPTIFVEVDPNAS
                     LSREEIFGPVLVVTRFTSEEQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(7446..8003)
                     /gene="puuR"
                     /locus_tag="ACHKIR_001799"
     CDS             complement(7446..8003)
                     /gene="puuR"
                     /locus_tag="ACHKIR_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HFO9989548.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(8030..8794)
                     /gene="puuD"
                     /locus_tag="ACHKIR_001800"
     CDS             complement(8030..8794)
                     /gene="puuD"
                     /locus_tag="ACHKIR_001800"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HFO9989549.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPGRDLLSMALINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            9006..10424
                     /gene="puuA"
                     /locus_tag="ACHKIR_001801"
     CDS             9006..10424
                     /gene="puuA"
                     /locus_tag="ACHKIR_001801"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HFO9989550.1"
                     /translation="METNIVEVENFVQQSEERRGSSFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGYLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDAEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            10573..10737
                     /locus_tag="ACHKIR_001802"
     CDS             10573..10737
                     /locus_tag="ACHKIR_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9989551.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            10727..12112
                     /gene="puuP"
                     /locus_tag="ACHKIR_001803"
     CDS             10727..12112
                     /gene="puuP"
                     /locus_tag="ACHKIR_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HFO9989552.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YDGDRTPVSET"
     gene            12246..12491
                     /gene="ymjA"
                     /locus_tag="ACHKIR_001804"
     CDS             12246..12491
                     /gene="ymjA"
                     /locus_tag="ACHKIR_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HFO9989553.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVADAERTPLALYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            12804..14447
                     /gene="sapA"
                     /locus_tag="ACHKIR_001805"
     CDS             12804..14447
                     /gene="sapA"
                     /locus_tag="ACHKIR_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein SapA"
                     /protein_id="HFO9989554.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQYIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            14444..15409
                     /gene="sapB"
                     /locus_tag="ACHKIR_001806"
     CDS             14444..15409
                     /gene="sapB"
                     /locus_tag="ACHKIR_001806"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine export ABC transporter permease SapB"
                     /protein_id="HFO9989555.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMVMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            15396..16286
                     /gene="sapC"
                     /locus_tag="ACHKIR_001807"
     CDS             15396..16286
                     /gene="sapC"
                     /locus_tag="ACHKIR_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HFO9989556.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            16286..17278
                     /gene="sapD"
                     /locus_tag="ACHKIR_001808"
     CDS             16286..17278
                     /gene="sapD"
                     /locus_tag="ACHKIR_001808"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HFO9989557.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            17280..18086
                     /gene="sapF"
                     /locus_tag="ACHKIR_001809"
     CDS             17280..18086
                     /gene="sapF"
                     /locus_tag="ACHKIR_001809"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HFO9989558.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            complement(18135..19298)
                     /locus_tag="ACHKIR_001810"
     CDS             complement(18135..19298)
                     /locus_tag="ACHKIR_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HFO9989559.1"
                     /translation="MARVSLSWALILGLLAGIGPMCTDLYLPALPEMSEQLAATTTIT
                     QLTLTASLIGLGVGQLLFGPLSDKIGRKRPLILSLLLFIVSSILCATTNNIYWLVVWR
                     FIQGIAGAGGSVLSRSIARDKYQGVTLTQFFALLMTVNGLAPVLSPVLGGYIVSTFDW
                     RTLFWVMAEISTVLLLGCLLFINETLPENKRGSSLLLTGRSVVQNRRFIRFCLIQSFM
                     LAGLFAYIGSSSFVLQKEFGFSPMQFSLVFGLNGIGLIIASWIFSRLARRINAMTLLR
                     GGLIAAILCALLTVLCAWIQLPIPALVALFFTIAFCSGIGTVGGAEAMSAVGTQESGT
                     ASALMGMSMFVFGGIAAPLSGIGGETLLKMSLAITVCYTLALLVALTRIDNQK"
     gene            complement(19307..20680)
                     /locus_tag="ACHKIR_001811"
     CDS             complement(19307..20680)
                     /locus_tag="ACHKIR_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HFO9989560.1"
                     /translation="MLRRSLIFLVLLSAGCVSLDPHYSTPESPIPATLPGAQGQGKAI
                     SHDWQQVIHDPRLQQVVTIALNSNRDVQKAIADIDSARALYGQTNASLFPTVNAALSS
                     TRSRSLANGTGTTAEADGTVSSYTLDLFGRNQSLSRAAQETWLASEFTAQNTRLTLIA
                     EISTAWLTLAADNSNLALAKETMTSAENSLKIIQRQQQIGTAAATDVSEAMSVYQQAR
                     ASVASYQTQVMQDKNALNLLAGTTLAENLLPGTLESLPEQMISLVPAGVSSDVLLRRP
                     DIQEAEHNLKSANADIGAARANFFPTISLTASAGVGSDALSSLFSHGMQIWSFAPSVT
                     LPLFTGGSNLAQLRYAEAQKRGLIATYEKTVQSAFKDVANALARRTTLEEQLDAQRQY
                     VKAEQQTVDVGLRRYQAGVGDYLTVLTAQRSLWSAQQELLALQLTDFTNRITLWQSLG
                     GGMSSLK"
     gene            complement(20684..23791)
                     /locus_tag="ACHKIR_001812"
     CDS             complement(20684..23791)
                     /locus_tag="ACHKIR_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HFO9989561.1"
                     /translation="MFSRFFVRRPVFAWVIAILIMLAGILAIRTLPVAQYPDVAPPTI
                     KISATYTGASAETLENSVTQVIEQQLTGLDNLLYFSSTSSSDGSVSINVTFEQGTDPD
                     TAQVQVQNKIQQAESRLPSEVQQTGVTVEKSQSNFLLIAAVYDTTDKASSSDIADWLV
                     SNVQDPLARVEGVGSLQVFGAEYAMRIWLDPAKLASYSLMPSDVQSAIEAQNVQVTAG
                     KIGALPSPNTQQLTATVRAQSRLQTVDQFKNIIVKSQSDGAVVRIKDVARVEMGSEDY
                     TAIGKLNGHPSAGVAVMLSPGANALNTATLVKDKIAEFQRNMPQGYDIAYPKDSTEFI
                     KISVEDVIQTLFEAIVLVVCVMYLFLQNLRATLIPALAVPVVLLGTFGVLALFGYSIN
                     TLTLFAMVLAIGLLVDDAIVVVENVERIMRDEGLPAREATEKSMGEISGALVAIALVL
                     SAVFLPMAFFGGSTGVIYRQFSITIISAMLLSVVVALTLTPALCGSVLQHVPPHKKGF
                     FGAFNRFYRRTEDKYQRGVIYVLRRAARTMGLYVVLGGGMALMMWKLPGSFLPTEDQG
                     EIMVQYTLPAGATAARTAEVNRQIVDWFLINEKANTDVIFTVDGFSFSGSGQNTGMAF
                     VSLKNWSQRKGAENTAQAIALRATKELGTIRDATVFAMTPPAVDGLGQSNGFTFELLA
                     NGGTDRETLLQMRNQLIEKANQSPELHSVRANDLPQMPQLQVDIDSNKAVSLGLSLND
                     VTDTLSSAWGGTYVNDFIDRGRVKKVYIQGDSEFRSAPSDLGKWFVRGSDNAMTPFSA
                     FATTRWLYGPERLVRYNGSAAYEIQGENATGFSSGDAMTKMEELANSLPAGTTWAWSG
                     LSLQEKLASGQALSLYAVSILVVFLCLAALYESWSVPFSVILVIPLGLLGAALAAWMR
                     DLNNDVYFQVALLTTIGLSSKNAILIVEFAEAAVAEGYSLSRAALRAAQTRLRPIIMT
                     SLAFIAGVMPLAIATGAGANSRIAIGTGIIGGTLTATLLAIFFVPLFFVLVKRLFAGK
                     PRRQE"
     gene            complement(23791..24912)
                     /locus_tag="ACHKIR_001813"
     CDS             complement(23791..24912)
                     /locus_tag="ACHKIR_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFO9989562.1"
                     /translation="MKYIATSVVAMLLLSGCDNTQSNNSSPSETEVGVVTLKSQPVSV
                     VSELTGRTSAALSAEVRPQVGGIIQKRLFKEGDLVKAGQPLYQIDAASYQAAWNEARA
                     ALQQAQALVKADCQKAQRYARLVKENGVSQQDADDAQSTCAQDKASVEAKKAALETAR
                     INLDWTTVTAPISGRIGISSVTPGALVTASQDTALTTIRGLDTMYVDLTRSSVDLLRL
                     RKQSLATNSDTMSVSLILEDGTTYSEKGRLELTEVAVDESTGSVTLRAIFPNPQQQLL
                     PGMFVRARVDEGVMEDAILAPQQGVTRDAKGNATALVVNKDNKVEQRTLETGETYGDK
                     WLVLNGLHNGDRLIVEGSAKVTSGQTVKAVEVQANGGNA"
     gene            complement(25061..25627)
                     /locus_tag="ACHKIR_001814"
     CDS             complement(25061..25627)
                     /locus_tag="ACHKIR_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFO9989563.1"
                     /translation="MTSKLEIRHKQRQDEIINAARRCFRRCGFHAASMSQIASEAQLS
                     VGQIYRYFANKDAIIEEMVRRIIDFRIAQMDIDARTDHFPEVLALRKSLNEDDDALML
                     EVAAEATRNPRVMAMLEEADARMFANGCAHMKRMHPHLSDEHIRCCVEVFATMMEGTV
                     YRRLTPQKSDPQHLQEIYQDIVSMLINK"
     gene            26105..26893
                     /gene="fabI"
                     /locus_tag="ACHKIR_001815"
     CDS             26105..26893
                     /gene="fabI"
                     /locus_tag="ACHKIR_001815"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFO9989564.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDTSIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            27037..28164
                     /gene="yciW"
                     /locus_tag="ACHKIR_001816"
     CDS             27037..28164
                     /gene="yciW"
                     /locus_tag="ACHKIR_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HFO9989565.1"
                     /translation="MEKRHITGKSYWYHETQSSTAEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTAWDC
                     VIINQIIGFIGFQARSIATFQAYLGHPVRWLPGLEIQNYADASLFADESIRWRSSYEV
                     EKLPEEHTKSSTAELCQLAETLSLHPISLSLLEKLLNSTRVNTQPDNQLAALLCARIN
                     GSPACFATCMNSSNEYKKISALLRKGENEINQWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            28232..30166
                     /gene="rnb"
                     /locus_tag="ACHKIR_001817"
     CDS             28232..30166
                     /gene="rnb"
                     /locus_tag="ACHKIR_001817"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008859 - exoribonuclease II activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HFO9989566.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDLVSDWLENTGDWKPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTDTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETAYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(30243..30347)
                     /locus_tag="ACHKIR_001818"
     CDS             complement(30243..30347)
                     /locus_tag="ACHKIR_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9989567.1"
                     /translation="MTYMKLTAAKRIVFFIYLFVIQFWKNASFQTKVS"
     gene            30401..32386
                     /gene="pdeR"
                     /locus_tag="ACHKIR_001819"
     CDS             30401..32386
                     /gene="pdeR"
                     /locus_tag="ACHKIR_001819"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFO9989568.1"
                     /translation="MKTVRESTTLYNFLGSHNPYWRLTESSDVLRFSTTETTEPERTL
                     QLSAEQAARIREMTVITSSLMMSLTVDESDLSVHLVGRKINKREWAGNASAWHDTPAV
                     ARDLSHGLSFAEQVVSEAHSAIVILDSRGNIQRFNRLCEDYTGLKEHDVIGQSVFKLF
                     MSRREAAASRRNNRVFFRSGNAYEVELWIPTRKGQRLFLFRNKFVHSGSGKNEIFLIC
                     SGTDITEERRAQERLRILANTDSITGLPNRNAMQELIDHAINQADNNKVGVVYLDLDN
                     FKKVNDAYGHLFGDQLLRDVSLAILSCLEHDQVLARPGGDEFLVLASNTSQSALEAMA
                     SRILTRLRLPFRIGLIEVYTSCSVGISLSPEHGSDSAAIIRHADTAMYTAKEGGRGQF
                     CVFTPEMNQRVFEYLWLDTNLRKALENDQLVIHYQPKITWRGEVRSLEALVRWQSPER
                     GLIPPLDFISYAEESGLIVPLGRWVILDVVRQVAKWRDKGINLRVAVNISARQLADQT
                     IFTALKQVLQELNFEYCPIDVELTESCLIENDELALSVIQQFSQLGAQVHLDDFGTGY
                     SSLSQLARFPIDAIKLDQVFVRDIHKQPVSQSLVRAIVAVAQALNLQVIAEGVESAKE
                     DAFLTKNGINERQGFLFAKPMPAVAFERWYKRYLKRA"
     gene            complement(32368..32496)
                     /gene="ymiD"
                     /locus_tag="ACHKIR_001820"
     CDS             complement(32368..32496)
                     /gene="ymiD"
                     /locus_tag="ACHKIR_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmiD"
                     /protein_id="HFO9989569.1"
                     /translation="MQKLPLKEKCLTATANYHPGIRYIITEYIAKYIYSSTYARFR"
     gene            32534..32707
                     /gene="yciZ"
                     /locus_tag="ACHKIR_001821"
     CDS             32534..32707
                     /gene="yciZ"
                     /locus_tag="ACHKIR_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciZ"
                     /protein_id="HFO9989570.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            32797..33546
                     /gene="yciT"
                     /locus_tag="ACHKIR_001822"
     CDS             32797..33546
                     /gene="yciT"
                     /locus_tag="ACHKIR_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YciT"
                     /protein_id="HFO9989571.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEIILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            33815..34033
                     /gene="osmB"
                     /locus_tag="ACHKIR_001823"
     CDS             33815..34033
                     /gene="osmB"
                     /locus_tag="ACHKIR_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HFO9989572.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(34159..34485)
                     /gene="yciH"
                     /locus_tag="ACHKIR_001824"
     CDS             complement(34159..34485)
                     /gene="yciH"
                     /locus_tag="ACHKIR_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HFO9989573.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGVDLDDAELTKLAAELKKKCGCGGAVKDGIIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(34485..35222)
                     /gene="pyrF"
                     /locus_tag="ACHKIR_001825"
     CDS             complement(34485..35222)
                     /gene="pyrF"
                     /locus_tag="ACHKIR_001825"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFO9989574.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDAALAFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAARFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(35413..36582)
                     /gene="lapB"
                     /locus_tag="ACHKIR_001826"
     CDS             complement(35413..36582)
                     /gene="lapB"
                     /locus_tag="ACHKIR_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HFO9989575.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMALLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(36589..36897)
                     /gene="lapA"
                     /locus_tag="ACHKIR_001827"
     CDS             complement(36589..36897)
                     /gene="lapA"
                     /locus_tag="ACHKIR_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HFO9989576.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVAPHSSAAKE
                     "
     gene            complement(37046..37810)
                     /gene="pgpB"
                     /locus_tag="ACHKIR_001828"
     CDS             complement(37046..37810)
                     /gene="pgpB"
                     /locus_tag="ACHKIR_001828"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HFO9989577.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            37980..38570
                     /gene="ribA"
                     /locus_tag="ACHKIR_001829"
     CDS             37980..38570
                     /gene="ribA"
                     /locus_tag="ACHKIR_001829"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HFO9989578.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(38634..41309)
                     /gene="acnA"
                     /locus_tag="ACHKIR_001830"
     CDS             complement(38634..41309)
                     /gene="acnA"
                     /locus_tag="ACHKIR_001830"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HFO9989579.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGNITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSTLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDDVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(41459..41554)
                     /gene="ymiC"
                     /locus_tag="ACHKIR_001831"
     CDS             complement(41459..41554)
                     /gene="ymiC"
                     /locus_tag="ACHKIR_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YmiC"
                     /protein_id="HFO9989580.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            complement(41668..41835)
                     /gene="yciX"
                     /locus_tag="ACHKIR_001832"
     CDS             complement(41668..41835)
                     /gene="yciX"
                     /locus_tag="ACHKIR_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciX"
                     /protein_id="HFO9989581.1"
                     /translation="MVGQEQLESSPLCQHSDNETETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQQKQ"
     gene            complement(41838..41978)
                     /gene="ymiA"
                     /locus_tag="ACHKIR_001833"
     CDS             complement(41838..41978)
                     /gene="ymiA"
                     /locus_tag="ACHKIR_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588449.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HFO9989582.1"
                     /translation="MRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVVVALLIWKV
                     WG"
     gene            complement(42297..43271)
                     /gene="cysB"
                     /locus_tag="ACHKIR_001834"
     CDS             complement(42297..43271)
                     /gene="cysB"
                     /locus_tag="ACHKIR_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HFO9989583.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEIMFKDIKLPEK"
     gene            complement(43481..46078)
                     /gene="topA"
                     /locus_tag="ACHKIR_001835"
     CDS             complement(43481..46078)
                     /gene="topA"
                     /locus_tag="ACHKIR_001835"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFO9989584.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            46458..>46705
                     /gene="yciN"
                     /locus_tag="ACHKIR_001836"
     CDS             46458..>46705
                     /gene="yciN"
                     /locus_tag="ACHKIR_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HFO9989585.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLV"
BASE COUNT        11160 a        12336 c        11574 g        11635 t
ORIGIN      
        1 aaacggcgca taagcgccgc ttagggtgga ttcttttaac ctttgacctt cggcattgcg
       61 atgtctttca ggccgccggc gttctcaacg tgggtatatc ccatctcgct aaggatctct
      121 tttgcctgcc ctgactggcg tccggcattg caatacactt tcacggtgtc gtttttatcc
      181 ggaacggcgg tggcaatgcg ctctttcact tccttcaggg gaatattgat ggctccctga
      241 acgtgctctt gctgatactg ctctggaaca cgaacatcga tccagtgttc agcggcgaaa
      301 acgggcagtg aaaacaccag tgtcagagca agtaagcctt ttttaaacat gtaagcgtcc
      361 ttctatggat gaaaattgcc atccataata cgccttagct ttaacaattt ttgcgaaact
      421 cccctcacct tttataacgc tgcgccagtt tattggcagc ccgacttaac agcggttcca
      481 gcgccacagc cagcaacatt ttcagtggtc ggcgagcaac tgattttatc gcccagccag
      541 ctacacccgc cgggccatag cgcagtgcgg taagaagtac cagcttgcct gctaatttga
      601 aaccaggctt caccttttgc ccggcctgtt gccagcgagt attcataact ttcctcacag
      661 ttgacggaaa cggctacgta acgtgaaagt atcggaagtg acataacgtt ccatctcgcg
      721 taaacgcgtt tcacttgccg ccagttcacg gtcgacttca tccagcaatt cactgctgga
      781 aggtagctgc tcaccaaagg ccatgttgtc cggcattgga tcaagcgcaa atgacaaaat
      841 gatgtaagca accagggtaa acagcgccag accgaagaaa atcgacagca ccaccaggat
      901 acgcaccagt tttactggta catcaaaata gttggcaatc ccggcgcaga cgccacggac
      961 catgccctgc tgtggaatac gccataattt tttattgaga ttaatgcccg ccattagcga
     1021 tccctccagt tcggatgttc tgcatcaaga atggattcca gcgcctgaat acgttcgcgc
     1081 atccgttttg cttcatcagc cagttgcgct aatcgctgct gctcactttg cgacaattca
     1141 ctgcgaccag aacgattgct gtaatgcagc cataaccaga tcggtaaaac aaacagcaca
     1201 aaaatggtta acggaatagc cagaaatagc gcgctcataa ggactcctta gacgatgagc
     1261 ggcggcgcgt tcggacgccg cctggatgtt attattgatt gtcttgcttc attttggctt
     1321 ttaattgtgc cagttgctcg ctgattgcat catcggcttt cagttcggca aactgatcgt
     1381 ccagcgattt ttgtttaccg aagctgtggc tttctgcttc cgcttccatc tggtcaattc
     1441 gacgttcgaa agattcgaaa cgagccattg cttcatccag tttgccactg tccagctgac
     1501 gacgcacatc gcgcgacgag tttgccgcct gatggcgtaa catcaatgcc tgctggcgag
     1561 cgcgtgtttc gctcaatttg ttttccagct cgccgatctc tttcttcatg cgtgccagcg
     1621 tatcgtccac cagcgtcact tcatgttcca ggctcttaat cagatcggtc agtttctgtt
     1681 tttcaattaa cgccgcacgc gccaggtctt ctctctcttt cagcagcgcc agttcggctt
     1741 tttcctgcca ttcaacttca cgcgccgacg cttgttcaat acggcgagtc agctgtttct
     1801 tttctgccag cgcacgcgcc gaagtagaac gtacttcaac cagtgtatct tccatctcct
     1861 ggatcatcag acgcaccagt ttctgtggat cttccgcttt ctctaacaga gcgttgatgt
     1921 tggcattcac gatgtcggca aagcgagaaa aaatacccat aatgttgtcc tcttgatttc
     1981 tgcgttcagg attgtcctgc tgaactatta atacaatttg cgtgccaatt ttttatcttt
     2041 ttgatttata aagatctgat tgaagaatca acagcaacat gccaggatga gttagcgaat
     2101 tacactaaca agtggcgaat ttcatcatgg cagaatacaa agataattta ctcggtgagg
     2161 cgaacagctt tctcgaagtg ctggaacagg tttcgcatct cgcaccgctg gataaaccgg
     2221 tgctcatcat cggcgagcgc ggcacaggta aagagctgat tgccagccgt ctgcattatc
     2281 tctcctcccg ttggcagggg ccgtttattt cccttaactg cgcggcgtta aatgaaaatc
     2341 tgctggattc cgaactgttt ggtcacgaag cgggggcgtt taccggtgcg caaaaacgtc
     2401 atccagggag atttgaacgt gccgacggcg gtacgctatt tcttgatgaa ctcgctacgg
     2461 caccgatgat ggtgcaggag aaattattgc gcgtgattga gtacggtgaa ctggagcgcg
     2521 ttggcggcag ccagccattg caggtgaatg tacggttggt atgcgcgacg aatgccgatc
     2581 tcccggcgat ggtcaatgaa ggtacttttc gcgctgacct gctcgaccgg ctggctttcg
     2641 atgtagtgca actgccgccc ctgcgcgagc gcgaaagcga cattatgctg atggcagaac
     2701 actttgccat tcagatgtgt cgggaaatca agctgcctct gttcccgggt tttacggagc
     2761 gcgccagaga aacattgctg aattatcgct ggccgggaaa tattcgtgaa ttgaaaaacg
     2821 tggtggaacg ttcggtgtat cgccacggca ccagcgatta tccgcttgat gacatcatta
     2881 ttgatccctt taaacggcgt ccgcctgaag acgctatcgc cgtttcagaa accacctcgc
     2941 ttccaacgct gccgctggat ttacgtgagt ttcagatgca gcaggaaaaa gagttgctgc
     3001 aactcagttt gcaacaaggg aaatataacc agaagcgcgc ggctgaatta ctggggttaa
     3061 cctatcatca gttccgcgcg ttgttgaaaa agcaccagat ttagcgaagt tgggcaatac
     3121 aacttgccgg atgcggcgtg aacgccttat ccggcataca taatcaatac gttgtttgca
     3181 ggcctgataa gcgcagcgca tcaaacatta ttgcgttggg ctattaatcg ctcagcgcat
     3241 cctgcaaaat tttcattgcc tcatcgaatt gcgcatccgg gatggtcagc ggatacagga
     3301 agcgaatcac gttgccgtat gcgccacagg tcagcaagag cagcccctgc gccagcgcgc
     3361 gttgctggat tttctgtgca atcgccgctg acggctcgcc cgtttgcgga tcgttaaact
     3421 ctgccgcaat catagacccc agaccgcgta ccgccgcgat ggccggaacg ctttctttgg
     3481 catcaatcaa cgtgtttgtc aggcgctggc ccagttgatt cgcgcgttca cagagtgatt
     3541 ctttgtcgat aatgttgagc acagcgtgcg cggcagccac cgccagcggg ttcccggcgt
     3601 aggtaccacc caacccgccc ggcgcgggcg cgtccataat attcgcgtta ccgaccacgc
     3661 ccgaaagcgg catcccgcct gcgaggcttt tcgccatcgt cattaagtcc ggcttatcgg
     3721 cgtagtgatc catggcaaac attttaccgg tacgcgcaaa gccgctttgc acttcatcgg
     3781 caatcatcac aatgccgtgc tcgtcgcaca ggcggcgaat ggcggcaacc agctcttttg
     3841 gcgcaacgtt gaaaccgcct tcgccctgaa ccggttcgaa aataatcgcc gccacctgct
     3901 tcgcttcaat gtccgattta aacaagcgtt cgatggcgtc gagggagtcc tgcgttgaaa
     3961 tgccgtgtaa atctgacgga taaggtacgt gatataccga accggggaac gggccgaagc
     4021 cgattttgta cggcgcgacc tttccggtca gcgccatggt catatatgta cgaccgtgga
     4081 agccgccgct aaacgcaatc acgccagggc gtccggtatg ggcgcgggcg attttcaccg
     4141 cgttctccac cgcttccgca ccggtggtga agaacgcagt tttagcctgc ccgctcaccg
     4201 gggcaagggc gttaattttc tccgccagcg tgacgtagct ttcgtacggc acaatctgat
     4261 acgccgtgtg ggtaaactgt tgcaattgct gttccaccgc cgcgaccaga tcagggtggc
     4321 gatgcccggt attcagtacc gcaatgcctg cggcgaaatc gatatattcg ttgccctcta
     4381 catccttcag cgtggcgttt tctgccgact gggcgaagaa gttacacatc acgccaaccc
     4441 cgcgtggggt agcagaaaga cgacgctgat ggaattcatt gttgctcatg tttcacacct
     4501 tttgactctt cactgttaat cggaaattaa acgcccagac gatcgcgcag gctgtaataa
     4561 gccgcgccca tcgcggtaaa cggaacgcgc agcgtgcgcc cgccggggaa cgggtaatgc
     4621 ggcagattgg cgaaggcgtc gaaacgctcg gcgtcaccgc gcagcagttc agcaatcaaa
     4681 cgcccggcga ggtgggtaca ggtgacgccg tggccgctgt agccctgcat gtagtagatg
     4741 ttggtatcga ggcgaccaaa ctgcggcatg cgtgacaggg tcagcaggaa gttgcccgtc
     4801 cagcggtaat caattttcac gcccttcagt tgcgggaagg ttttcagcag tttcggcacc
     4861 acaaggcgct caacatcgtc cgggtcgcgt gcgccgtaga ccacgccgcc gccgtacagc
     4921 aggcggttat cggcggtaag acggtagtaa tcaagcagat agttacagtc ttccacacag
     4981 tagtttttcg ggatcagcga acgggctaaa tcttccgaca gccgttcggt ggtgatcacc
     5041 tgcgtgccgc acggcatgct gcgtttcgct agttccggct ctactttatc gcccaggtag
     5101 gcattcccgg cgacaatcac atacttcgcc gtcacctgac cattggcagt ccgcaccacg
     5161 gctggcgtgg tgtgctggat ctgcgtcact gcggaaagct catgcacgcg cccgccgttc
     5221 aggcggatgg catcggcttc accaatggcg aggtttagcg ggtgaatatg cccaccgctg
     5281 tgatccagca gcgcaccagt gtagcgatcg ctggcgacct cgcggcgaat agcgttggcg
     5341 tccagcagct ccagctgttt attgccgtag cgttcccagt tctctttctg ctcttcgagt
     5401 gtggcgagct gtttatcatt catcgccaca aacagaccgc cggggcgata gtcgcagtca
     5461 atctgataac gtttgatacg ttcgcggatg atctcgccac cctcgaacat catgctgccg
     5521 agcatacggg cggtatccat gccgtagctt ttttcgatca cgtcgatgtc gcggctgtag
     5581 gagttcacaa gctgcccgcc gttgcgtccg ctcgcgccaa agccgatgcg tgaggcttcg
     5641 agaaccacca cgtcaaagcc cgcttccgcc agatgcagcg cggaggaaag cccggtatag
     5701 ccgccgccga ccacgcaaac gtcgcaggaa atcgactcat tcagcgtgtc gaatggcgcg
     5761 tatttattcg cactggcggc gtagtaactg ctggtgtgtt cggtcatttc aggcctccag
     5821 gcttatccag atggttttca gttcggtgaa tttttcaagg gcatgcaggg atttgtcgcg
     5881 cccgttgccg ctctgcttat agccgccaaa cggcacggtc atatcgccgt cgttgtagtt
     5941 attgacgaag acggagccgg ctttcaggcg gcgactcatg cggtgcgcgc gggagaggtc
     6001 gcgcgtccat accgccgcgc caagtccgta ctggctgtcg ttggcaagct gtagcgcctg
     6061 ttcttctgat gtgaaacgcg tgaccaccag caccggaccg aaaatctctt cgcggcttaa
     6121 ggacgcattc gggtctacct ccacaaagat ggtcgggccg atggcggcag ccagctcggc
     6181 gttacggcca tccagcagca gttgcccttt gctttcgcct tcccgaataa agctatggac
     6241 cgagtcggcg tgggcgcagt cgattaaggt gcccatggtg gttgcgggat caagcggatg
     6301 gcccggctgc cagttttgcg cttgctgttt taacagggct aagaattcat cggcgatgct
     6361 ctcttccagc aacaggcgcg ttccggcgat acacacctgc ccctggttgt agaaaatgcc
     6421 cgcagcggtc gcgctcgccg ctttttgcaa atccggacag tcggcgaaaa cgatgttggc
     6481 gcttttgccg cccgcttcca gccagacgcg tttcatgttg ctgtcgcccg cgtccttcag
     6541 cagctgtttc ccggtgcggg ttgagccagt aaaggcaatg gcatcgatat cgttatggcg
     6601 cgaaagcgcc tgcccggctt catgaccaaa acccgtcacc acgttcaaca caccatccgg
     6661 cagccccgct tctttcgcca gccccgcgag acgaatcgca ctgagcggtg atttttcaga
     6721 cggttttaga atcacgctgt ttcccgccgc cagcgccggg ccgagtttcc agcaagtcag
     6781 cagcagcggg aagttccacg gcacgatggc ggcaatcacg ccgaccggtt cacgcacgat
     6841 catcgccagc tcatggctac tggtggtcgc cacttcgcca tacactttgt cgatcgcttc
     6901 ggcgtaccag cgaatggcgc gcgccgcgcc gggaatatca tcacgcaggc tgtgacgaat
     6961 cggtttaccg gtgtcgagcg tttccagcag cgccagttct tcagcgttgg cttccattaa
     7021 atcggcgagt ttattcagca ccgctttacg cttagccgga gaagagagtg accagtcgcc
     7081 acgttcaaac acgccgcgtg ctgcgctcac cgcacggtca atatcgacgc tcttgccgcg
     7141 ggcaatattc gccagcggtg cctgggtgac cggatcaacg gtttcaaaag tttcattttc
     7201 cgccgcagca gtatattcac cgttaataaa taagcgggtt tcaatggcga gacttaacgc
     7261 tttatcctgc cagtaagcca gatgatgaaa attcattatg actcctgttt cacgtctatc
     7321 agatatatgc agtttgtgaa cgtcagggcg cagagatccc cgggccgtat gcccgtttat
     7381 ttctggcgac attctttata gattgccggg cgcattgcgc ttacccggct tacagaacaa
     7441 aaagattaaa acgtggtggg cgtatgggcg ctgataattc ggcaaatacc tgccgacgta
     7501 ttactgaaac tgtgcgggat gccggtatta atggcataac tttgccccgc gacgagatga
     7561 taatcctgac cattaatcgt cagaacaatt tcaccttcca gtacagtgcc tatttcctca
     7621 ccctgatgct taattctttc cccagtggtt gtgcccggct ggtaagtttc aaagatcatc
     7681 gccagcgtgc gattcgggtt accgttatga accagcttca ttgacacacc ctgactgccc
     7741 atctcaatta agtcgtcctg attaatgacg acctgcggct catcaggttt ttccggctcg
     7801 gaaaagaatt ccgagagtga cagaccatac accttcagca gcttttgcag cgtactgatg
     7861 gcagggctga ctttatcttg ttctatcgta ctgatagcac tgtgagtcag cccggagagt
     7921 tcggcggcac gacgttgtga aagcccctgc tgctggcgga tttccgacaa gcgttttcct
     7981 ggcgccagtc cctcatcact catatttgca tttccttaaa ctgtagtggt cagagtcgct
     8041 gtttttcagc gatatggtgc tgacaagcgg tgataaagcc ctcgaacaat atacgcgaaa
     8101 gcgcgtactc gctactgttc cattccgggt gccactgtac gcccagcgca aaaggatgat
     8161 tgatgacgct aaccgcctca actaagccat ccggcgagcg agcttcaaca cgcaaccgtg
     8221 ggctaacgac cttcgcccct tgtccatgca gagagtttac ccaaaagttg ctacattcag
     8281 gtaacaacgc agacagtaat cccccctctt caacctgaac ttcatgcgac ggcgcatatt
     8341 gctgctccac cggcagttcg ggatcttccc gatgttccag caattcaggc tgttcgcaca
     8401 gcttgcgatg caacgaacca ccggttgcca ccaccagttc ctgtaaaccc cggcagatgg
     8461 cgaaaatggg gatgcgcctt tcgagtgcgg cattaattaa cgccatgctc agaagatcac
     8521 gcccaggatc ggcgtcaggc tcatcgccgt tttcaccata taggtgcggc tgcacattgc
     8581 tgggactacc aggaagataa atgccatcga gtttcggcaa aagttgttca agtaatgacg
     8641 gttccgccag cgcatgtggc agcgcaattg gcaagccgcc tgcgtggatg atggcattca
     8701 ggtacttttc ttgcagagtc tgggtcgcat gacccttaag cctgttcctg cacattacga
     8761 caccgataac cggattgttc attatatttt ccatgctcaa tctcacaaag tggactaaat
     8821 tatcgccatt actgcgcttt aaatgacctt tatgttcaat attttttcaa tctagcagtg
     8881 ggttttcatt tttgcaaact caatttaaca tttgacaaac atttagtttg catacagatt
     8941 cgaatagtgg tcattatatt ttacgctttg ataacgagcg gaaaacaaac caaaggcgaa
     9001 gaatcatgga aaccaatatc gttgaagtag agaactttgt tcagcagtca gaagagaggc
     9061 ggggtagctc ctttacgcag gaagtgaaac gctacctgga gcgctacccg aatacgcaat
     9121 atgttgatgt tttacttacc gatttgaatg gttgcttccg tggaaaacgt attcctgttt
     9181 caagcctgaa gaagctcgag aaagggtgtt atttcccggc ctcggtgttt gcaatggata
     9241 ttctgggcaa cgtagtagaa gaagcgggcc tgggtcagga aatgggcgag ccggatcgta
     9301 cctgtgttcc tgttctcggt tccttaaccc cttctgccgc cgatccagag tttatcggtc
     9361 agatgctcct gaccatggtc gatgaagatg gcgctccctt tgacgttgag ccgcggaacg
     9421 ttcttaaccg cctgtggcag cagctgcgcc agcgcggatt gttcccggtc gtagcggtag
     9481 agctggagtt ctatttactg gatcgccagc gcgacgctga aggatatctg caaccgccct
     9541 gcgcgcccgg caccgatgac cgcaatacac aaagccaggt ttactccgtt gataacctca
     9601 accacttcgc cgacgtgctc aatgatattg atgagctggc gcagttacag ctgattccgg
     9661 cagatggcgc ggtcgccgag gcctcgccag gtcagtttga aatcaatctt taccataccg
     9721 ataacgtgct ggaagcttgc gatgatgcgc tggcactaaa acgcctggtg cgtctgatgg
     9781 cagaaaagca taagatgcac gccactttta tggcgaagcc gtatgaagag cacgcgggca
     9841 gcgggatgca tatccatatc agtatgcaaa ataatcgtgg cgagaacgtg ctttctgacg
     9901 cggaaggcga agattcgccg ctgctgaaaa agatgctcgc cgggatgatt gacctgatgc
     9961 cgtcatcaat ggcgttgctg gcaccaaacg tgaactcgta tcgccgcttc cagccgggga
    10021 tgtatgtacc gacgcaggcg tcgtggggcc ataacaaccg caccgtcgcc ctgcgtattc
    10081 cctgcggcga tcgccataat caccgcgtgg aatatcgcgt ggcgggtgcc gatgccaacc
    10141 cgtatctggt gatggcggcg atttttgccg gtattttgca tggccttgat aacgaactgc
    10201 cattacagga agaagtcgaa ggtaacgggc tggaacagga aggcttaccc ttcccgattc
    10261 gccagagcga tgccctgggt gagtttatcg agaatgatca cctgcgccgc tatttaggcg
    10321 aacgcttctg ccatgtctat cacgcctgta aaaatgatga gctgttgcag tttgagcgcc
    10381 tcatcacaga aacagaaatt gagtggatgt tgaaaaacgc gtaatacccc ttgcggggtt
    10441 gtttcatcgg cctgagcgga gccaggcctg acatttgctg gtaatccaga catttcccgg
    10501 atgtcgcgtt atggagcgca ggcggcaacg ggcttccgtt aacgattaag actgtgacga
    10561 ggaaatgaga tgatgccgat gattaaatcc ccgcatggcg agggaggctg cgtatgcgcc
    10621 cctcctgcga ctgactggac tccgccccct ctgcttccct tgctaaacag gtttgatttc
    10681 aggtcgacac gaccgcaaac cttattacgc aggggaggca gcaattatgg ctattaattc
    10741 accactgaat attgctgcgc aacctggcaa aacccgtctg cgaaaatcac tgaaattgtg
    10801 gcaggtggtg atgatgggtc tggcctatct cacgccgatg accgtatttg atacttttgg
    10861 tattgtctcc ggcattagcg acggtcacgt tccggcgtcc tatttgctgg cgctggcggg
    10921 cgtgctgttt accgctatca gctacggcaa actggttcgc cagtttccgg aggccggttc
    10981 ggcctatacc tacgcgcaaa agtcgattaa cccgcacgtc ggatttatgg tcggctggtc
    11041 atcgctgctg gattatctct ttttgccgat gatcaacgtt ttgttggcga aaatctatct
    11101 ctccgccctc ttcccggaag tgccgccgtg ggtgtgggtg gtaaccttcg ttgccatttt
    11161 aaccgccgcg aatctgaaga gcgtcaacct ggtcgctaac ttcaatacct tgtttgtact
    11221 ggtgcaaatc tccatcatgg tggtgtttat cttcctggtt gttcagggac tgcataaagg
    11281 agaaggcgtt ggcaccgtct ggtcacttca gccgtttatc agcgagaacg cgcacctgat
    11341 cccaattatt accggggcga cgattgtctg tttctccttc ctcggtttcg atgcggtgac
    11401 cacgctttcg gaagagacgc cggacgccgc acgcgtgatc ccgaaagcca tcttcctgac
    11461 ggcggtatac ggcggcgtta tctttatcgc tgcgtcgttc tttatgcagc tgttctttcc
    11521 cgatatcagc cgctttaaag acccggacgc cgcactgcct gaaattgcgc tatacgtcgg
    11581 cggcaagctc ttccagtcga ttttcctctg taccacgttt gtgaacacgt tagcgtctgg
    11641 cctggcgtcg cacgccagcg tgtcgcgtct gctgtatgtg atggggcgtg acaatgtgtt
    11701 tccggagcgc gtgtttggct atgtgcaccc gaaatggcgg actccggcac tgaacgtcat
    11761 tatggtcggg attgtcgcgt tgtcagcgct gttctttgat ttagtcaccg cgacagcatt
    11821 gattaacttc ggtgctctgg tggcatttac cttcgtaaat ctgtcggtgt ttaatcattt
    11881 ctggcggcgt aaaggaatga ataaaagctg gaaggatcac ttccactatt tgctgatgcc
    11941 gctggttggc gcgctgacgg tgggtgtgtt gtgggttaac ctcgagtcaa cgtcactgac
    12001 actcggtctg gtatgggctt cgttaggcgg cgcatatttg tggtatctga tccgccgcta
    12061 tcgcaaagtg ccgttgtatg atggtgacag aacgccggtg agcgaaacgt aatagtcgga
    12121 tgcgcggcgc attatcctgc ctgagggtaa tgcaccgcgc cattaactat aaaaagatgt
    12181 atgacccgga tcacgagtgc tatcatccgc ccttatgctt agcgcattaa ccccaggggc
    12241 aaataatgaa ccacgatatc ccgctaaaat attttgatat tgccgatgag tacgcgaccg
    12301 aatgcgcaga gcctgtcgca gacgctgaac gcacaccgct ggccctctac ttccagttac
    12361 tgctcacccg tttgatgaac aatgaagaga tcagcgaaga agcccaacat gaaatggctg
    12421 ctgaagcggg gattaatccc gtacgcattg atgagatcgc agagtttttg aatcaatggg
    12481 gtaatgagta attatttcat tgcagactct cggtcagtat cgttgtccgg ataaagcgtt
    12541 cacgccgcat ccggctaaca atgacacgca ctgcagcgtg cctcaatggg tttctcacga
    12601 tcgtaccgct ctgctcctcg ctaagttgtt ctgtgagtta tagttataga gaaatcacgg
    12661 gtacgaatgt tgaacagatg gcgacgtcat ctgttcaacg accaaccaca cggccctcat
    12721 tacgacccct gaacacaaac ggtaatggca aatgtcatta aatattagta agatagcctc
    12781 tttacataaa atccattaat attatgcgcc aggtattatc gtctcttttg gtgattgctg
    12841 gacttgtgag tggtcaggca atcgccgcgc ctgaatcccc cccgcatgct gatatccgcg
    12901 acagcggttt tgtctattgc gtcagcgggc aagtcaacac ctttaaccca tccaaagcga
    12961 gcagtgggtt aattgtcgat acccttgccg cccagtttta tgatcgactg ctggatgtcg
    13021 atccctatac ctatcgcctg atgccggaac ttgccgaaag ctgggaagta ctcgacaacg
    13081 gcgcgaccta tcgcttccac ctgcgtcgcg atgttccgtt tcaaaaaacc gactggttta
    13141 ctcccactcg taaaatgaat gccgacgatg tggtgtttac cttccagcga atttttgacc
    13201 gcaacaaccc gtggcataac gtcaacggca gcaacttccc ctacttcgac agcctgcaat
    13261 ttgccgataa cgtgaaaagc gtccgtaaac tggataatca taccgttgag ttccgtctgg
    13321 ctcagccgga tgcttctttt ttgtggcacc tcgcaaccca ttatgcttcg gtcatgtcgg
    13381 cagaatatgc ccggaagtta gagaaagaag atcgccagga gcaactcgac cgtcagccgg
    13441 tcggcactgg accgtatcag ttgtcggaat accgcgccgg gcaatatatt cgcttacaac
    13501 gtcatgatga tttctggcgt ggtaagccgt tgatgccaca agtggtggtg gatttaggct
    13561 ctggcggtac aggacgattg tcgaaactcc tgaccgggga gtgcgacgtt ctggcctggc
    13621 ctgctgccag tcagctatcc attttgcgtg acgacccacg cttgcgttta acgctgcgcc
    13681 cggggatgaa cgtcgcctat ctggcgttta acaccgctaa accgccgcta aataaccccg
    13741 ctgtccgcca tgcgctggca ctggcgatta ataaccagcg cctgatgcaa tccatctatt
    13801 atggtacggc tgaaacggcg gcctctattt taccgcgcgc ctcgtgggcc tatgacaacg
    13861 aggctaaaat tactgaatac aatccggcaa aatcgcgcga acagttgaag gcgttggggc
    13921 tggaaaactt aacgctgaaa ctgtgggtgc caacacgttc gcaggcgtgg aaccccagtc
    13981 cgctgaaaac cgccgaactg attcaggcgg atatggcaca ggttggcgta aaagtggtga
    14041 ttgtgccggt agaaggtcgt tttcaggagg cgcggttgat ggatatgagc catgatctga
    14101 cgttatccgg ttgggcgacg gacagtaacg acccggacag tttcttccgt ccgttactga
    14161 gctgcgcggc aattcattca cagaccaacc tcgcccactg gtgcgatccg aaattcgaca
    14221 gcgtgttgcg taaggcgctc tcctcgcagc agctggcggc gcgtattgaa gcctatgacg
    14281 aagcgcagag tattctggcg caggaattac ccattctgcc gctggcgtcg tcattgcgct
    14341 tgcaggccta tcggtacgat atcaaaggtc tggtacttag cccgtttggt aacgcctcct
    14401 ttgctggggt gtatcgcgag aaacaggatg aggtgaaaaa gccatgatta tcttcacctt
    14461 gcgccgtatt ttgttattga ttgtcacctt gttcctgctg accttcgttg gcttcagctt
    14521 gagctatttc accccacatg cgccgttaca aggcgcgtca ctgtggaatg cctgggtgtt
    14581 ctggtttaac ggcctgatcc actgggattt tggtgtttcc agcattaacg gtcaaccgat
    14641 tgccgaacag ttaaaagagg tcttcccggc gacgatggaa ctgtgcatcc tcgccttcgg
    14701 ttttgcgctg attgttggca tccccgtagg aatgattgcc gggattacgc gccataagtg
    14761 gcaggataac ttgatcaacg ccatcgcctt actgggcttt tcaatcccgg tgttctggct
    14821 ggcgcttctg ctgacgctgt tttgttcact cacactaggt tggttgcccg tttcagggcg
    14881 tttcgatctg ctctacgaag tgaaaccgat taccggtttt gcgttgattg atgcctggct
    14941 ttcggactca ccatggcggg atgaaatggt catgagcgca atccgccata tgatattgcc
    15001 cgtgatcacc ctgtcggttg cgccaacaac tgaagtgatc cgactgatgc gtattagcac
    15061 tatcgaagtg tatgaccaga actatgttaa agcagcggcg acccgcggtt tgtcgcgctt
    15121 tactattttg cgtcgccacg ttctgcataa cgcgttacct ccggttattc ctcgcctggg
    15181 cttacagttt tctaccatgc tgacgctagc gatgatcacc gaaatggttt ttagctggcc
    15241 gggtttaggg cgctggttaa ttaacgccat ccgccagcag gattacgcag ccatttccgc
    15301 cggagtgatg gtgtgtggct cactggtcat tattgttaac gtgatttctg atattttggg
    15361 tgccatggct aaccctctga aacataagga atggtatgcc ttacgatagc gtatacagcg
    15421 aaaagcgccc gccgggcacg ctgcgtaccg cctggcgcaa attttatagt gacgcctctg
    15481 cgatggtcgg cctgtacggc tgcgcggggc tggctgtact gtgtattttt ggcggctggt
    15541 ttgcgcctta cggcatcgac cagcaatttc tcggttacca attattgccg ccgtcatggt
    15601 cgcgctatgg cgaagtttct ttcttcctgg ggactgacga cctcggacgc gatgttttaa
    15661 gccgtttact gagcggagct gcgccaaccg taggtggcgc atttgtggtg acgcttgccg
    15721 cgacgatctg tggcctggtt ctgggcacct tcgccggggc gacccacggc ctacgctcag
    15781 cggtgcttaa ccatattctg gataccttac tggcgatccc ttcgctgctg ctggcaatta
    15841 tcgttgtagc gtttgccgga ccgagtttgt ctcacgccat gtttgctgtc tggctggcat
    15901 tgctgccgcg tatggtacgt tcgatttaca gcatggtgca tgacgaactg gaaaaagagt
    15961 acgttatcgc cgcccgtctg gatggcgcat caacgctgaa tattctctgg tttgctgtga
    16021 tgcccaacat caccgccggg ctggtcaccg agatcacccg cgcactgtcg atggcaattc
    16081 tcgatatcgc cgcgctgggc tttctcgatc tcggcgcaca actcccctcg cctgaatggg
    16141 gagcgatgct cggtgatgcg ctggaactga tttatgtcgc cccgtggact gtcatgctgc
    16201 caggtgcggc aattatgatt agcgtgctgt tggttaacct ccttggcgac ggtgttcgtc
    16261 gtgcaattat tgcgggggtg gaataatgcc attactcgat attcgtaacc tgaccattga
    16321 atttaaaacc ggtgatgagt gggttaaagc cgtcgaccgc gtaagtatga ccttaaccga
    16381 aggtgaaatc cgcggtcttg ttggcgaatc cggttccggc aaaagtttga ttgcgaaagc
    16441 gatttgtggg gtgaataaag ataactggcg tgttactgct gaccgtatgc gttttgatga
    16501 tatcgatttg ctgcgtctct ccgcacgcga acggcgcaag ctggttggtc acaacgtgtc
    16561 gatgattttc caggaaccgc agtcttgtct tgacccttca gaacgtgtgg gtcgccagtt
    16621 gatgcaaaac atcccagcct ggacgtataa aggccgttgg tggcagcgtt ttggctggcg
    16681 caaacgccgt gccattgaac tactgcaccg tgtggggatt aaagatcaca aagatgcgat
    16741 gcgcagtttt ccctatgagt tgaccgaagg tgaatgtcag aaagtgatga tagccattgc
    16801 actggcgaat caaccgcgtc tgctgattgc tgacgaaccg acaaactcaa tggagccaac
    16861 aacccaggcg caaatctttc gcctgctgac gcgtctcaac cagaacagca ataccactat
    16921 tttgcttatc agccatgact tacaaatgct tagccaatgg gcggataaaa ttaacgtgct
    16981 ttactgcggt caaacggtgg aaaccgcgcc aagtaaggag ttggtgacaa tgccacatca
    17041 tccttatacc caggcgctga tccgcgcgat accagacttc ggcagcgcga tgccgcataa
    17101 aagtcgcctc aatacgctgc ccggcgctat cccactgctg gaacagttac cgattgggtg
    17161 tcgtctgggg ccgcgttgcc cgtatgcaca acgagaatgc attgtgacgc cacgtttgac
    17221 gggggcgaaa aatcatctct atgcctgtca cttcccgctg aacatggaga aagagtgaga
    17281 tgatcgaaac gctgcttgaa gtgcgtaatt taagtaaaac gttccgttac cggaccggct
    17341 ggtttcgtcg tcagaccgta gaagcggtaa aacctttgag ctttacgcta cgtgaaggcc
    17401 agacactggc gattattggt gagaatgggt cgggtaaatc cacactggca aaaatgctgg
    17461 cgggaatgat agaaccgacc agcggcgagt tattgattga cgatcatccg ctgcattttg
    17521 gcgattattc cttccgtagt cagcgtattc gcatgatttt tcaggatcct tcgacctcgt
    17581 tgaatccccg tcaacgtatc tcgcagattc ttgattttcc actgcgcttg aacaccgatc
    17641 tggaacctga acagcgtcgc aaacagatta ttgaaacgat gcggatggtt gggctgctac
    17701 cggatcacgt cagttactat ccgcatatgt tggcgcccgg acaaaaacaa cgtctgggtc
    17761 tggcacgcgc gctgatattg cgtccaaaag tgattattgc cgatgaagcc ctcgcctcac
    17821 tggatatgtc gatgcgttcg cagttgatta atctgatgct ggaattacag gaaaaacagg
    17881 gtatttcgta tatttatgtt acccagcata tcggaatgat gaagcacatt agcgaccagg
    17941 tactggtgat gcatcagggc gaggttgtcg agcgaggcag caccgccgat gtgctggcat
    18001 cgccgctgca tgaactcacc aaacggctta ttgccggtca ttttggcgag gcattgacgg
    18061 cggatgcgtg gcgtaaggac cgttagtggt ttgcacctca tcctgacagg gcccccctca
    18121 ggatgaggtg ttcattactt ttgattgtcg attctggtga gagcaaccag caatgccagc
    18181 gtataacaca ccgtaattgc cagactcatt tttaacagtg tttctccgcc aattcccgac
    18241 aatggcgcgg ctataccgcc gaagacaaac atgctcatcc ccatcaacgc agacgccgtt
    18301 ccagattcct gcgtccctac tgcactcata gcctctgccc cgccaacagt gccgatgccg
    18361 ctacaaaatg cgatggtgaa aaataatgcc accagtgccg gaatgggcaa ttgtatccat
    18421 gcgcataaga ccgtgagcaa tgcacacaaa attgccgcta tcaggccacc tcgcaacaat
    18481 gtcatcgcgt taatccgtcg cgccaggcgc gagaagatcc atgaagcaat gataagtccg
    18541 atgccgttaa ggccaaacac caggctaaat tgcattggac taaagccaaa ttccttctgc
    18601 aacacgaacg acgaagagcc gatatatgca aacaaaccag ccagcataaa actttgaatc
    18661 aggcaaaagc gtataaaacg gcggttctgc accacgcttc gtccggttaa tagcaatgat
    18721 gagccccttt tattttctgg caaggtctca ttaataaata acaggcagcc cagcaacagt
    18781 acggtgctaa tttcagccat tacccagaat aaagtgcgcc agtcaaaagt gctaacaatg
    18841 tacccgccca gcactggcga caacaccggt gccaggccat tcaccgtcat taacagcgca
    18901 aaaaactggg tcaacgttac tccctgatat ttgtcacgag caatagaacg agagagcacc
    18961 gaaccacccg cccccgcgat cccttgaata aaacgccaga ccaccagcca gtaaatattg
    19021 ttcgttgtcg cgcataaaat ggaagaaaca ataaacaata gcaacgacaa gattaacgga
    19081 cgtttacgcc ctattttgtc gctcagaggg ccaaataaca gttgtccgac gccaagacca
    19141 atcagtgatg cggtcagggt taattgcgtt atggtcgtgg ttgccgccag ttgctcagac
    19201 atctccggca aagccggcaa ataaagatcg gtacacatcg ggccgatacc ggctaaaaga
    19261 ccaagaatca atgcccatga aagagagact ctagccatat tattccttac ttgagtgatg
    19321 acataccgcc gcccagcgac tgccagagcg tgattcggtt tgtaaaatca gtcagttgca
    19381 gtgccagcag ctcctgctgt gcgctccaca agctgcgctg cgccgtcaac acggtcaggt
    19441 aatcaccaac acccgcctga tagcgacgca agccaacatc gaccgtttgt tgctcagctt
    19501 taacatactg gcgctgtgca tccagttgct cttcaagcgt ggtccgccgc gccagtgcgt
    19561 tagcgacatc tttaaatgcg ctctgaacgg ttttttcata ggtggcaatg agcccacgct
    19621 tttgtgcttc tgcgtaacga agctgcgcca gattacttcc gccagtgaac agcggtaatg
    19681 tgaccgatgg cgcaaatgac cagatctgca ttccgtggct gaacaacgac gacaatgcgt
    19741 cactgccaac accggcactg gcagtcagcg aaatcgtcgg gaaaaagtta gcccgcgccg
    19801 cgccaatatc tgcattggcg ctcttcaaat tatgttcagc ttcctggata tccgggcgac
    19861 gcagcagaac atcagatgat actcccgcag gaaccagact tatcatttgt tccggcaggc
    19921 tttccagtgt tcccggtagc agattttccg caagcgttgt acctgccagt aagttcagcg
    19981 catttttatc ctgcatcact tgtgtctgat agctggcaac gctggcgcgc gcttgctgat
    20041 aaacactcat cgcctcactg acatctgtcg ctgccgccgt gccaatctgt tgctggcgct
    20101 ggataatttt caatgagttt tctgcactgg tcatcgtttc ttttgccagg gccaggttgc
    20161 tgttatcagc cgccagtgtc agccaggcgg tgctgatttc agcaatcagc gttaaacgag
    20221 tgttctgggc tgtaaattca ctggcaagcc aggtttcctg cgccgcccga gataaactct
    20281 gattacgacc aaacagatct aacgtataac tggacaccgt gccgtcagcc tcggcagttg
    20341 ttcccgtacc attcgccagc gaacgactgc gagtcgaact taaggccgca ttcaccgtcg
    20401 gaaataacga cgcattagtt tgcccataga gcgcccgtgc tgagtcaata tcagcaatcg
    20461 ctttttgcac atcgcggtta ctgttcagtg caatggttac tacctgctgc aatcggggat
    20521 cgtggatcac ctgctgccag tcatggctga ttgccttccc ctggccctgt gctcccggca
    20581 aggtcgccgg gattggggat tcaggcgtac tgtagtgggg atcgagcgag acacatcccg
    20641 cactcaacaa caccagaaaa ataagagaac gacgcaacat aacttactcc tgacggcgcg
    20701 gtttaccggc aaacaaacgt ttcaccagta caaaaaacag aggaacaaaa aaaatagcca
    20761 gtaatgtagc ggtcagcgtg ccgccaataa tgcccgtacc aatggcgatg cggctgttcg
    20821 cccctgcacc ggttgctatc gccagcggca ttacccccgc aataaacgcc agcgaggtca
    20881 tgatgattgg gcgtaaacga gtctgcgccg cgcgtaatgc cgcacgactc aaggaatagc
    20941 cctctgcaac cgccgcttca gcaaattcca caatcagaat ggcgtttttc gacgacaagc
    21001 cgatagtggt taatagcgcc acctggaagt aaacatcgtt gtttaaatca cgcatccagg
    21061 ccgccagcgc cgcgccaaga agccccagag ggatcaccag aatgaccgag aacgggactg
    21121 accagctttc gtacagtgct gcaaggcaga ggaaaaccac cagaatagaa actgcataca
    21181 gactcaatgc ctgaccgctg gccagtttct cctgcaatga caaaccactc caggcccagg
    21241 ttgttcccgc aggaagactg tttgccagtt cctccatttt tgtcattgca tcgccggaac
    21301 taaagccagt cgcattttcc ccctgaattt cataggctgc agaaccgttg tagcgcacca
    21361 gtctttccgg tccatacagc cagcgggtgg tcgcaaaagc agagaatggt gtcatggcgt
    21421 tatcgctacc gcgcacaaac catttaccta agtctgacgg cgcggaacga aattcactgt
    21481 cgccctgaat gtagactttt ttcacacgac cacggtcaat aaagtcattc acataagtac
    21541 cgccccacgc actggacagg gtgtcggtga catcattcaa actcaaccct aatgacaccg
    21601 ctttattact atcaatatct acctgcaatt gcggcatttg tggcaaatca ttggcacgca
    21661 cagaatgtaa ctccggactt tgattcgctt tttctatcaa ctgattacgc atttgcagta
    21721 gtgtttcgcg atcggttcca ccgttagcta acaattcaaa cgtaaaacca ttgctttggc
    21781 ccagcccatc aacggctggc ggcgtcatcg caaataccgt ggcatcacga attgtgccca
    21841 gctctttggt agcccgtagg gcgatagcct gggcggtgtt ttctgcccct ttacgttgag
    21901 accagttttt caacgaaaca aacgccatcc cggtgttctg tccgctgccg ctaaaactga
    21961 aaccatcaac ggtaaaaatg acatcggtat ttgctttctc gttaatcagg aaccagtcaa
    22021 caatctggcg attcacttct gctgtacggg cagcggtcgc cccggccggc agcgtgtact
    22081 gcaccatgat ttcgccctgg tcttcagtgg gtaagaaact gcccggcagt ttccacatca
    22141 tcagggccat tccaccaccc agtacgacat aaagccccat cgttcgagca gcacggcgca
    22201 ggacataaat tacgcctcgc tgatatttat cttcagtacg gcggtaaaag cggttaaatg
    22261 cgccgaaaaa gcctttttta tgtggcggaa catgctggag gacggaaccg cacagggcgg
    22321 gagtcaaggt caatgccacc accacggaaa gcagcattgc ggagatgatg gtgatggaga
    22381 actgacgata aattacgccc gtagatcccc caaagaaggc catcggcagg aatacggctg
    22441 acaacaccag cgcaatggca accagtgcgc cagaaatctc gcccattgat ttttccgtgg
    22501 cttcacgcgc gggcaaccct tcgtcacgca taatgcgttc gacgttctct accaccacaa
    22561 tggcatcgtc caccagcaag ccgatcgcca gcaccattgc aaacaacgtc agggtattaa
    22621 tcgaatagcc aaacaacgca agaacgccga atgtgcccag caaaacgacg gggacagcta
    22681 acgccggaat cagtgtggca cgcaggtttt gcaggaataa atacatcacg caaaccacca
    22741 atacgatggc ttcaaacagc gtctgaatta cgtcctctac ggagatttta ataaattcag
    22801 tgctgtcttt cgggtatgcg atgtcgtatc cctgcggcat gttccgctgg aattcggcaa
    22861 tcttatcctt gaccagcgtc gccgtattca gcgcattcgc accgggcgaa agcattacag
    22921 caaccccggc agacgggtga ccgttaagtt tgccgatagc ggtataatct tcactgccca
    22981 tctcaacgcg agccacatct tttatacgaa caactgcgcc gtctgactgg cttttcacga
    23041 taatattttt gaactgatcc accgtctgca aacgagactg cgcacgtacc gttgcggtca
    23101 gttgctgagt attcggtgaa ggcaatgccc ctattttccc ggcagtaacc tgcacgtttt
    23161 gcgcttcaat agcactttgc acgtctgaag gcattagcga gtaagacgcc agtttggccg
    23221 ggtcaagcca gatgcgcata gcgtattccg cgccaaagac ttgcagactc cccacacctt
    23281 caacacgcgc cagcgggtcc tgaacgttac taaccaacca gtcggcgata tccgaactgg
    23341 aggctttgtc agtggtgtca tacacggcag caatcagcaa aaagttgctt tgtgatttct
    23401 ccaccgtaac acccgtttgc tgcacttcgc tgggtaggcg cgactccgcc tgctgaattt
    23461 tattctgcac ctgaacctgt gcagtatctg gatcggtacc ttgttcaaag gtcacattaa
    23521 tactgaccga accatcagag ctgctggttg agctgaaata gagtaaatta tcaagcccag
    23581 tgagttgttg ctcgataacc tgagtcacgc tgttctccag cgtttcggca gaagcaccag
    23641 tataagtggc tgaaatttta atggtcggtg gcgcaacgtc aggatattgc gcgacgggca
    23701 atgtgcgaat agccagaatc cccgccaaca taatcaaaat ggcgataacc caggcaaaga
    23761 ccgggcggcg aacaaaaaag cgcgaaaaca tcaggcgttg cctccattag cctgaacttc
    23821 aacagccttg acggtctgcc ctgaagtgac tttggcagaa ccttcaacaa tcagtcggtc
    23881 gccgttgtgc aggccgttca gcaccagcca tttatcacca tacgtttctc ccgtttcgag
    23941 cgttcgctgc tctactttat tgtctttatt caccaccagc gcagttgcat tgcctttagc
    24001 atcgcgcgtg acgccctgtt gcggcgcgag aatagcgtct tccatcacgc cttcatcgac
    24061 acgagcgcga acaaacattc ccggtaataa ctgctgttgt ggattgggaa aaatcgcccg
    24121 taatgtcacc gaaccggtag actcatctac agcgacttcg gtgagttcca gacgcccttt
    24181 ttcgctgtaa gttgtgccat cttccagaat aagtgagacg ctcatggtgt cactgttggt
    24241 cgccagtgac tgtttacgca gacgtaataa atcgacacta gagcgagtga ggtcgacata
    24301 cattgtatcc agaccacgaa tagtcgttaa cgctgtatcc tgcgacgcgg tcaccagtgc
    24361 gccaggggtt accgacgaaa taccaatacg ccccgaaatc ggtgcggtta ccgtggtcca
    24421 gtcaagatta atgcgcgcag tttccagtgc ggcttttttc gcctctacac tggctttatc
    24481 ttgtgcacag gtagactgag catcatcagc atcctgttgt gaaacaccgt tctctttcac
    24541 cagtcgggca taacgctgcg ctttctggca atcggctttt accagtgcct gtgcttgttg
    24601 taatgctgcc cgagcttcat tccatgcagc ctgataactg gccgcatcaa tctggtagag
    24661 cggctgtcca gccttgacca gatcaccttc cttaaataag cgtttctgga taattccccc
    24721 aacctgcgga cgtacttcag cactgagcgc agcactggtg cgtccggtta attcactgac
    24781 taccgaaacc ggttgagatt tgagcgtaac aacgccgact tctgtttctg acggggatga
    24841 attgttactt tgcgtattat cacaacccga taagagcagc attgccacta cagatgttgc
    24901 tatatacttc attattttct ccagggtgag aaataccctc ctcattgaaa ttaatgagga
    24961 tatgtcaggg atattcagtt ataacgaacc cgcatcgcga aatgaatttc gcaatgaaat
    25021 aagggggatt aatatccccc gctattggtt aattctgttc ttatttattt atcaacatac
    25081 taacaatatc ctgatatatt tcctgcagat gttgtggatc gcttttttgc ggtgttaaac
    25141 gacgataaac cgtaccttcc atcatggtag caaacacttc aacgcagcaa cgaatgtgct
    25201 cgtcgctaag atggggatgc attcttttca tatgtgcaca accgttagcg aacattctgg
    25261 cgtcagcttc ttcaagcatt gccataaccc gcgggttgcg tgtggcttct gcagccactt
    25321 ccagcatcag cgcgtcgtcg tcctcattta aggatttacg caaggcaagg acttccggga
    25381 agtgatcggt acgcgcgtca atgtccattt gggcaatacg aaagtcgatg atacggcgaa
    25441 ccatctcttc aataatggcg tctttgttgg cgaagtaacg atatatctga cccacgctaa
    25501 gctgagcttc gctggcaatt tgcgacatac tggcggcatg aaatccgcag cgacgaaaac
    25561 accgacgggc ggcgttaatg atttcatcct gacgctgttt gtggcgtatc tccagcttgc
    25621 tggtcatttc tgtctcctgg cctatcactt gatgagaatg atcgttctca tttttgctaa
    25681 ttatacgttt ctccaaagta atggaaagta tttttatctt attcatgatg catattctga
    25741 taatgaagag attgtggaga ttctgtgaag atagttgaca atattaagtt ggggtcaatt
    25801 catcagaaag acagcgactt gttgtcgcca gttctgtttt catcatcaaa tttatcggag
    25861 ctaatacgaa tagtttcata gtcagattct ataagatatt tccccaacct cccccttgca
    25921 ggcgtcagga gcagataacg caaaatgctc tgtaccgcag cttctctccg ctacagaaag
    25981 cgcaactata gccacccaca gccaggttga ttataataac cgtttatctg ttcgtactgt
    26041 ttactaaaac gacgaatcgc ctgattttca ggcacaacaa gcatcaacaa taaggattaa
    26101 agctatgggt tttctttccg gtaagcgcat tctggtaacc ggtgttgcca gcaaactgtc
    26161 catcgcctac ggtatcgctc aggcgatgca ccgcgaagga gctgaactgg cattcaccta
    26221 ccagaacgac aaactgaaag gccgcgtaga agaatttgcc gctcaattgg gttctgacat
    26281 cgttctgcag tgcgatgttg ccgaagatac cagcatcgac accatgttcg ctgaactggg
    26341 gaaagtttgg ccgaaatttg acggtttcgt acactctatt ggttttgcac ctggcgatca
    26401 gctggatggt gactatgtta acgccgttac tcgtgaaggc ttcaaaattg cccacgacat
    26461 cagctcctac agcttcgttg caatggcaaa agcttgccgc tccatgctga atccgggttc
    26521 tgccctgctg accctttcct accttggcgc tgagcgcgct atcccgaact acaacgttat
    26581 gggtctggca aaagcgtctc tggaagcgaa cgtgcgctat atggcgaacg cgatgggtcc
    26641 ggaaggtgtg cgtgttaacg ccatctctgc gggtccgatc cgtactctgg cggcttccgg
    26701 tatcaaagac ttccgcaaaa tgctggctca ttgcgaagcc gttaccccga ttcgccgtac
    26761 cgttactatt gaagatgtgg gtaactccgc ggcattcctg tgctccgatc tctctgccgg
    26821 tatctccggt gaagtggtcc acgttgacgg cggtttcagc atcgctgcaa tgaacgaact
    26881 cgaactgaaa taatcgttct gttggtcaag acgggcggcg ttctgccgcc cgttatctct
    26941 gttatacctt tctgatattt gttatcgccg atccgtcttt ctccccttcc cgccttgcgt
    27001 caggataacg atttccttta cgaccaagga gcgcccatgg aaaaacgcca cattaccggc
    27061 aaaagctact ggtatcatga aacgcaatcc agtactgcgg agtatgacgt tctgcctctg
    27121 gtcccggaag ccgcaaaggt cagcgatccc tttctgctcg acgtgatcct tgatgaagaa
    27181 acgctggccc ccttcctttc atggctggtc cctgcgcgcg ttcttgcagt ggaattgttc
    27241 cctgaccagc ttaccgtgac ccgttcacag accttcaccg cttatgaacg cttgtcgacg
    27301 gccctgacgg ttgctcaggt ttgcggcgtc caacggttat gtaactacta ttcggcgcga
    27361 cttacgccgc tccccgggcc tgattcctcc agggaaagta atcatcggtt agcacaaatc
    27421 acgcaatatg cccgccaact ggctagctcg ccttctatta tcgacaaccg atcgcgccag
    27481 catctgaatg acgtcggcct aactgcctgg gactgtgtaa tcattaacca aatcattggt
    27541 tttattggct ttcaggcgcg gtcaattgcg acatttcagg cttatctcgg gcatccggta
    27601 cgctggttac ccggtctgga gatacaaaac tatgccgacg cgtcactgtt tgctgatgaa
    27661 tcaatacgct ggcgaagcag ctatgaggtg gaaaaactcc ctgaagagca cacaaaaagt
    27721 tcaactgcag aactttgcca actggccgaa acactctctc tccaccctat ttcactttcc
    27781 cttctcgaaa agttgctaaa cagcacacgg gtcaatacac agccggataa tcagcttgcg
    27841 gcgttgttat gcgcgcggat aaatggcagt cctgcttgtt ttgccacctg tatgaattca
    27901 tcaaatgaat ataaaaaaat cagcgccctt ctgcgcaagg gcgaaaatga aattaaccaa
    27961 tgggctgacc gtcattctgt tgagcgcgct accgttcagg cgatacaatg gctgacccga
    28021 gcacccgatc gctttagcgc cgcccagttc agcccattac tcgaacacga aaaatcatca
    28081 acgcagatta ttaatctgct ggtatggagc gggctgtgtg gctggataaa tcgcttaaaa
    28141 atcgcgttgg gtgagacata ttaaccttgc cgcgtcagac agattcgcgt aaaactgtca
    28201 gccgctctaa tggccaccaa aatagacaat tatgtttcag gacaacccgc tgctagcgca
    28261 gcttaaacag caactgcatt cccagacgcc acgcgctgaa ggggtggtaa aagccacaga
    28321 aaaaggcttt ggcttcctgg aagtcgacgc gcaaaaaagt tatttcattc cgccgccgca
    28381 gatgaaaaaa gtcatgcatg gcgaccgaat tatcgcggtt atccacagtg aaaaagaacg
    28441 tgaatccgca gagccagaag aactggttga accgtttctg actcgtttcg tgggtaaagt
    28501 tcagggcaaa aatgaccgtc tggccatcgt tcctgatcat ccactcttaa aagacgccat
    28561 tccttgccgc gcagcccgtg gcctgaacca cgagtttaaa gaaggcgact gggcggttgc
    28621 cgaaatgcgc cgtcatccgc tgaaaggcga tcgttctttc tatgctgaac tgacacaata
    28681 catcactttt ggtgacgacc actttgtacc gtggtgggtt acccttgcgc gccataatct
    28741 ggagaaagaa gcaccagacg gcgtcgctac cgaaatgctc gatgaaggtc tggttcgtga
    28801 agatctgacc gcgctggatt ttgtcaccat cgacagtgcc agcacagaag atatggatga
    28861 cgcccttttc gctaaggcgt tgccggatga caaacttcag ctgattgtgg cgattgccga
    28921 tccaaccgcg tggattgctg aaggcagcaa gctggacaaa gccgcgaaaa ttcgcgcatt
    28981 caccaactat ctgcctggct tcaacatccc tatgctgcct cgcgagcttt ctgacgatct
    29041 ctgctcactg cgcgccaatg aagtccgccc ggtactggca tgccgcatga cgctctccgc
    29101 tgatggcacc attgaagata atatcgaatt ctttgccgcc accatcgaat ccaaagcgaa
    29161 gctggtgtat gacctggttt ctgactggct ggaaaatacc ggtgactgga agcctgaaag
    29221 tgaagcaatt gctgaacaag tccgtttgtt agcgcaaatt tgccaacgcc gcggcgagtg
    29281 gcgtcataac cacgcactgg tgtttaaaga tcgcccggat taccgcttta ttctcggtga
    29341 aaaaggtgaa gtgctggata tcgtcgccga gcctcgtcgc attgccaacc gtatcgtcga
    29401 agaagcgatg attgccgcta acatttgtgc ggcccgcgta ctgcgcgata agctcggttt
    29461 tggcatctat aacgtgcata tgggctttga tccggcgaat gccgacgcgt tggcagcgtt
    29521 gctgaaaacg cacggtctgc atgtcgatgc tgaagaagtg ctcacgctgg acggtttctg
    29581 caaactgcgt cgtgaactgg atgcgcaacc aactggtttc ctcgacagcc gcattcgtcg
    29641 cttccagtca tttgctgaaa ttagcactga acccggtcct cactttggcc tcggtctgga
    29701 ggcatacgcc acctggactt cgccgatccg taaatatggc gacatgatca accaccgtct
    29761 gctgaaagcg gttatcaaag gcgaaactgc gacgcgtccg caggatgaaa tcactgtcca
    29821 aatggccgag cgtcgccgtc tcaaccgaat ggcagaacgt gatgttggcg actggttata
    29881 cgcacgcttc ctgaaagaca aagccgggac cgacacccgt ttcgcggcgg aaattgtcga
    29941 tatcagccgt ggcggcatgc gtgtgcgttt ggttgataac ggcgctatcg cctttattcc
    30001 ggcacctttc ttacacgctg tgcgcgatga actggtttgc agccaggaaa acggcactgt
    30061 acaaattaaa ggtgaaacgg cttacaaagt gactgacgtt attgacgtca ccattgccga
    30121 agtccgcatg gaaacccgca gcattattgc gcgcccggtc gcgtaatctc ctttcacggc
    30181 ccattcctta tgaatgggcc gtttatttcc ccgctctacc ttcatcatat cccggcagtt
    30241 ttttaactga ctttcgtttg aaaactggcg tttttccaaa actggattac aaataaatat
    30301 ataaaaaaaa caatgcgttt agccgccgtt aatttcatgt aagtcatgtt gttcctttta
    30361 tttaaccgcg gactccgctg ttaaccggag gatatgcatc atgaaaaccg ttagggagtc
    30421 cacaacgttg tacaactttc tcggatcgca caatccatac tggcggttaa cggaaagcag
    30481 cgatgttttg cgcttttcta ccaccgaaac cacagaacct gaacgtacgt tgcagttatc
    30541 tgccgaacag gctgctcgca tcagggaaat gacggtcatc acctccagcc tgatgatgag
    30601 tctgaccgtc gatgaaagcg atctttctgt gcatctggta ggacgaaaaa tcaataaacg
    30661 ggaatgggct ggcaacgcgt ctgcatggca tgacacacca gcagttgctc gtgatttatc
    30721 acacggactt tcctttgctg agcaggtagt ttctgaagca cattccgcaa tagtgattct
    30781 cgacagccgg gggaatatcc aacgcttcaa tcggttatgt gaagattaca cagggttgaa
    30841 agaacatgac gtcattgggc aaagcgtgtt taaactgttt atgagccgtc gtgaagctgc
    30901 ggcatccagg cgcaataacc gtgtattttt tcgaagcggc aatgcatatg aagtcgaact
    30961 gtggatacca acacgtaaag gccagcggct gtttctgttt cgcaataaat ttgtccacag
    31021 cggcagtggc aagaacgaaa tttttttaat ctgttccggc accgacatta ccgaagagcg
    31081 ccgcgctcag gagcgactgc gtattctggc aaataccgac agtatcaccg gactgccgaa
    31141 tcgtaacgca atgcaggagt taatcgatca cgccattaat caggcagata acaataaagt
    31201 tggggttgtg tatcttgatt tggataattt taaaaaggtc aacgacgctt atgggcattt
    31261 gtttggtgac cagctattac gcgacgtgtc attggccatt ttaagctgtc tcgaacatga
    31321 tcaggtgttg gcgcgtccag gtggcgatga gtttctggta ctggcatcca atacctcaca
    31381 aagcgcgctg gaagcaatgg catcacgaat tttgacccgc ttacgactcc ccttccgcat
    31441 tggtttaatt gaagtttata ccagctgttc agtgggtatt tcactctctc ccgaacatgg
    31501 ttcggacagc gcggctatta ttcgtcacgc cgacacagca atgtacacag cgaaggaagg
    31561 cggacgagga caattttgcg tttttactcc agaaatgaat cagcgggtat ttgaatatct
    31621 ctggctggat accaacttgc gtaaagcact ggaaaacgat cagttggtta ttcactatca
    31681 accgaaaatc acctggcgtg gcgaagtgcg cagtctggaa gcactagtac gttggcagtc
    31741 acctgaacgt ggattgattc caccgttgga cttcatttcc tacgccgaag agtcagggct
    31801 aattgtgcct ttaggccgtt gggtgattct cgatgtagta cgccaggtgg caaagtggcg
    31861 ggataaaggt attaacctgc gagtggcggt aaacatttct gcacgtcagc tcgccgatca
    31921 aaccattttc accgccctga aacaggttct ccaggaactc aattttgaat actgccctat
    31981 tgatgttgaa ctgacggaga gctgcctgat tgagaatgat gagctggcac tgtctgttat
    32041 tcaacaattt agccaactag gtgcgcaagt gcatctggac gattttggta ccggttactc
    32101 ttcactttcg caactggcgc gctttccgat cgatgccatc aaacttgacc aggtttttgt
    32161 tcgagatatt cacaaacaac ctgtctcgca gtcactggtc cgggcgatcg tcgctgtggc
    32221 ccaggcattg aatcttcagg tgatcgccga aggtgtagag agtgcaaagg aagatgcttt
    32281 tttaaccaag aacgggatca atgagcggca aggatttttg tttgccaaac cgatgcccgc
    32341 cgtcgccttc gaacgttggt ataaacgcta tctgaagcgc gcataagtag atgaatatat
    32401 atacttagcg atatattccg taattatata tcgtattccc ggatgataat tagccgttgc
    32461 tgttaaacac ttctctttta gcggcaattt ttgcattatt cattttccca cttttgctat
    32521 aaggatgtcc ttgatgtctg aatttgatgc ccagcgcgtt gccgaacgta ttgatattgt
    32581 gttagatatc cttgtggcag gcgattacca ctctgcgatc cataatctcg agatacttaa
    32641 agcagaactc ttgcgccagg ttgctgaatc tacgccagac atcccgaagg caccgtggga
    32701 gatttaacaa tcgtgccttc aggttttcct atttgaggat gcccggcgcg tcagtatttt
    32761 tttatttagt attataacgt tataagaatt acagcgatga attcccgaca acaaactatt
    32821 ctacagatgg tcattgacca gggtcaggtt agcgtaaccg atctggcaaa agccactgga
    32881 gtttctgaag ttaccattcg ccaggatctc aacaccctcg aaaaactgag ttacctccgc
    32941 cgtgcacatg gctttgcagt atcgcttgat agtgatgacg tcgaaacccg tatgatgagc
    33001 aactatacgc tgaagcgtga actcgccgag tttgccgcgt cactggttca accgggcgaa
    33061 accatcttta tcgaaaatgg cagcagcaat gccctgctcg cccggactct gggcgagcag
    33121 aagaaaaatg tcactatcat cacggtcagc agctacatcg cgcatttgct gaaagacgcg
    33181 ccttgtgaaa ttattttgct cggtggcgtg taccagaaaa aaagcgaaag tatggttggc
    33241 ccgttgacac gccagtgtat ccaacaggta catttcagca aagcatttat tggtattgat
    33301 ggctggcaac ctgaaactgg attcaccggt cgcgatatga tgcgtaccga tgtggtcaat
    33361 gccgtgctgg aaaaagagtg cgaagcgata gtcctgactg acagctcgaa atttggtgct
    33421 gtacattcat actccatcgg tcccgttgag cgattcaatc gcgtgattac cgattcgaaa
    33481 atacgcgcca gcgatctgat gcatcttgag cacagtaaac tcaccgttca cgttgttgac
    33541 atttaaccat ttcccttctc tacggatgat ttgcactttg gcaaatcatc cgctctaaga
    33601 tgattcctgg ttgataatta agactattta cctgttatta acactctcaa gatataaaat
    33661 tattatcagc gatataacag gaagtcatta tcacctgcgt gatataaccc tgcgcgcgag
    33721 cggatttcac ggaataattt caccaggctt attcttagct attatagtta tagagagctt
    33781 acttccgtga atcataaatt caggagagag tattatgttt gtaacgagca aaaaaatgac
    33841 cgcggctgtt ctggcaatta ccctggcaat gtctctgagt gcctgttcta actggtctaa
    33901 acgggatcgc aacaccgcaa tcggcgcggg tgcaggggca ttaggcggtg cagtactgac
    33961 cgatggcagt acgttgggta cattaggtgg cgcagctgtc ggtggtgtta ttggtcacca
    34021 ggtcggtaaa taatcgtaaa taaatgtcga ttatcgactc gcaattataa tacgcttgat
    34081 tctgttcagt acgaaataac tgaataatac acacagtaaa atattcgaaa gccacggata
    34141 tatccgtggc tttttatgtt aaccaccggc gagttttacc ttcatccctt tcgcttcaag
    34201 cagtgatttt aataaatcac gcttatcgcc ctggatttca ataattccat ctttaactgc
    34261 cccgccgcag ccgcattttt tcttcagttc cgctgccagt tttgtcagtt cggcatcatc
    34321 gagatcgaca ccggtaatca ggcaaacgcc cttaccttta cgtccactgg tctgacgctg
    34381 aatacgcacc acaccgtcgc ctttagggcg tacaggggcc gcttttggct catcaatacg
    34441 tccggtttcc gttgagtaga ccagacggct gttggaatca ctcatcatgc actccgctgt
    34501 aaagaggcgt tgatcgcttt cagcgtttgc gctggatcta ccgattgcgt taccgggcga
    34561 ccaatcacca tataatcaac accagccgcc aacgcctgtt ctggcgtcat aatgcggcgc
    34621 tggtcaccag cttcactccc ctgcggacga atgcccggcg taaccagttt gaactcctga
    34681 ccaaatacct gtttaaagcg cgcagcttcc tgagcagaac acaccacacc atcaaggcca
    34741 catttttgcg tcagtgccgc cagacgttct gcataatctg caggtgacag tgtcacgcca
    34801 agatcggcca ggtcgctggc ttccatgctg gtcaatactg tcacagcaat caaaagcggt
    34861 gcatctttgc caaacggcac cagtgcctca cgcgctgcgg tcatcatacg cgccccaccg
    34921 gaggcgtgaa cattcaccat ccacacgcct aagtcagctg cagcagcgac agcgtgcgct
    34981 gcagtgttgg gaatatcgtg aaatttcagg tcgaggaaga tatcaaaacc acgctgttga
    35041 agttcgcgta caaattgagg tccaaacagc gtaaacatct ctttgccgac tttcagacga
    35101 caatcgcgtg ggtcgatctt gtcgacaaag gccagcgcgg catcacgatt atgataatca
    35161 agggcaacaa ccacaggaga attcgtaaca gcgcgggaag aagatgaagc agttaacgtc
    35221 atgaccagac cttcttgatg atgggcaccg gaagtggcgc gaaacaggta aacggcgagc
    35281 attctacccg tggaaataaa aaattaacag ttgcgatttc tcccctggca ggcttttctg
    35341 cgccggagtt accggagcac aatgaacatc aattattagt atgttgtaac taaagtcgga
    35401 tttttttaaa aattacaggc catcaagacc gcgaatcggt ttaatggttg accaggcccg
    35461 acaagacgga caatgccagt agagggtgta tgcggtaaaa ccacatttct ggcagcgata
    35521 acgaggctta ctacgcacct tctcgccaac catgtcacgc agcaccatca gactctcttt
    35581 ggcacgccct tcttccgctt catttaagtg gtaatccatt agcttatgga acacacgcat
    35641 ggtcggatga cgctgaagct gacgcgtaat atagacctgt gcggcctcac taccgtcgcg
    35701 cgcttcgata atatccgcaa gcatcaattc agcatcggca ccggtgttct cttccaccgc
    35761 gcgctgcagg aattctgccc attcggcagt tttacccaac tgctgatagc aggtttgcaa
    35821 catttccagc gtttcgctga ccagttctct gtcctgagat atcacgcgtt gcagactttc
    35881 gacggctttg gcgtattctc ctttcgccat aaatacgcgt cccatcatga tggatacgcg
    35941 ggcgctgttt ttatctgccg ccgcaccttt ttttaacaag gccatggcgc gatcgagatc
    36001 gtcgctggcc atatgctgca gggctaactc acagtagaaa tgggcaattt cgacgcgctg
    36061 cttatcttta cccaacttca ccagacgttc ggcaacatca attgctttct gccactcgct
    36121 ggtagcctgg tagatttgta gcaactgttg cagcgcgcca atgcggaagt cagtttcatc
    36181 ggtcagctga ttgaacatgt cttccgcgcg gtcgtataac ccggcggcca tgtaatcacg
    36241 ccccagttgt tgaatcgcca acagacgctg ttcataggtc agcgaggcgc tttccattag
    36301 ggtctgatgg atgcgaatag cgcgatcaac ttcgccacgc gaacggaaca ggtttcctag
    36361 cgtaaggtgg gcttcaacgg tacccgtatc ctctttaagc atatcgagaa acaggtctac
    36421 cgctttatcc tgttgattac taagcaggaa gttaaccccc gctacgtaat cacgcgacaa
    36481 gcggttggct tcatcttgct tgttttgttg cgcacttctg cggcccatat accagccata
    36541 ggcagcggct acaggcaaaa gcagaaacaa caactccagc atagaaagtt attccttcgc
    36601 cgctgacgaa tgcggcgcta cagccacgtc agtcgcgggt gaaagctggt tttccagtcg
    36661 ctttatttta cgttcagcgc gcgccaggga aacacgaact cgcagccaga acagaccgca
    36721 aatcagccaa ccgatagcaa accccgcagc aaacaatacc gccagcaatg tggaaatacg
    36781 gtactcccct tgcgctaaca gataattaaa cgtcacctgt tgatcgttct gcgcacctaa
    36841 cgtgaccgaa atcacgaaga tcgctaacac cagtaagaaa atgagtaaat atttcacatt
    36901 acttcccgtt atgtggttag agcgaataaa ttatggtcaa cttaccgcaa tagcccttta
    36961 aagtaccatt ttcgcgccgt ctgcgaaatg gcatccacgc gcattgatga tgatttaagg
    37021 gctaaattcg gaaaatcaac cagcgttaac tttcttgttc tcgttgcgct atttcgcgat
    37081 tttcttccgc aggtggtgtt aatggcccac aaattcgttg cgcaagccac gttgccaccg
    37141 ccaccagcgc ccacgaaatc aacgtagcta ccaccagatc gcgtggccaa tgcatcccga
    37201 gcagcaggcg gcttcccatg actcccgttg cccagaccaa caagatagca atggttaacg
    37261 ttcgccgacg cggccacagc aaaccaacgg ccagcagcgc ccaactggca gcaaacatcg
    37321 tgtgaccgga aggaaaggca aaccccgtct ctttctgcca gtgtgaacgc aaatattgtg
    37381 ggatattttt ctcttcagcc aactgttctt tcaccagatt tccgcgttct gctcgcttta
    37441 aagtgtagaa ctcatcaacc ggaatatgat gtgttttttc cagccagata acaaaaggtc
    37501 gtggttcctg gactttgtct ttgatccagg atttaacgcc ttgtcccaca agaattgccg
    37561 ccgccagaat ggcaaataat acaaaggcag ccttaatgcg aaaacgcaga caccagagaa
    37621 accagccgaa taaaatcaaa tgtgtaatga cgccccaggg ctgggtgaca gtttcagtaa
    37681 cccaaaaagc cgcttttagt agccaacttt gttctccagg ttgccaacgc caaccagaaa
    37741 tccatacggc tactggcatg acaagcaata gtgcagctcc cactgcggta cgtctggcaa
    37801 tcgaacgcat ggcctctcct ttttgataag tcccacaatc ataactgaaa acgccagttc
    37861 caggaaaaat tgacagattt gtgccattcc gtgaacgatc gacgcgtcgt gattaggtga
    37921 accccttctc gttatggcaa aataagccaa tacagaacca gcattatctg gagaatttca
    37981 tgcagcttaa acgtgtggca gaagccaaac tgccaacccc atggggcgat ttcctgatgg
    38041 tgggatttga agaactggca accggacacg atcatgtcgc gctagtctat ggcgatatct
    38101 ccgggcatac cccggtactt gcgcgcgtcc attccgaatg tctgaccggt gacgccctgt
    38161 tcagcttgcg ctgcgattgt ggcttccagc tcgaagcggc actgacgcaa attgccgaag
    38221 aaggccgtgg tattttgctg tatcaccgtc aggaaggtcg taacattggt ctgctgaata
    38281 aaatccgcgc ttatgcactg caggatcaag gttacgatac tgtagaggct aaccaccagt
    38341 taggcttcgc cgctgatgag cgcgacttca ctctttgcgc tgatatgttc aaactccttg
    38401 gcgtcaatga agtccgcttg ttaaccaata acccgaaaaa agtcgaaatt ctgaccgaag
    38461 cagggattaa tattgttgaa cgcgtaccat tgattgtagg tcgtaacccc aataacgaac
    38521 attatctcga taccaaagcc gagaaaatgg gccatttgct gaacaaataa ccctcttgca
    38581 ttgtgtaatt catttgcttg ccggaagcaa aataaccggc aagcaaatac ttgttacttc
    38641 aacatattac gaatgacata atgcaaaatg ccgtcgttct ggtagtaggt caactccgtc
    38701 gcggtgtcga tacgacaacg gcagggtacg acttcctggc taccatccgc gcgcgtaagc
    38761 gtcaccggaa ccgtcgcgcc gggttgtagg ttttgcagat cgccaatatc aatcttctct
    38821 tccccggtta gccctaacgt tttacgcgtt acgccttgcg gaaattccag cggcaggatg
    38881 cccatgccaa ttaaattcga acggtgaatt cgttcaaacg attcggcaat caccacacga
    38941 ataccaagca gacgcggacc ttttgccgcc cagtcacggc tggagcctga tccatactct
    39001 ttcccggcaa tcaccgccag cggcgtttgc tcctgcttat agcgcatcgc ggcatcatag
    39061 atagagacat catcgctgtc aggtaaatgc cgcgtcatcc ccccttcaac gccaggcacc
    39121 atttcattac ggatgcgaat attggcgaag gtgccgcgca tcatcacttc atggttacca
    39181 cgccgcgaac cgtaggagtt aaagtctttt cgctcaacac cccgaccttg tagatatcga
    39241 cccgccgggc tgtcgggctt aatactgccc gccggagaga tatggtcagt ggtgactgaa
    39301 tcccccagca ttgcgaggat ccgcgcaccg tgaatatctt ccacaggtgc tggtgttgcc
    39361 tgcatttcat caaagaatgg cgataagcga atataggttg aatcttcctg ccaaccgtag
    39421 gtatcggatc gtgtgacgtt aatttccttc cactctgctg tgccttcaaa aacttctgcg
    39481 tactctttgc ggaacatttc tgtggagact tgttctaccg cacgggcaat ttcttgtgcc
    39541 gagggccaga tatctttcag atataccggg tcgcctttac gatcgtgacc aataggctca
    39601 gaagcgaggt taatattcat atttcccgcc agcgcatagg caaccaccag cggcggcgag
    39661 gccagccagt tagttttaac cagcggatgg atacggcctt caaagttacg gttgccggac
    39721 agcaccgcac cgacggttaa atcgcctttt ttgattgccg tttcgatagg atcgggcagc
    39781 ggcccagagt taccaataca ggtggtacaa ccgtacccca caaggttaaa ccccagttcg
    39841 tcgagatacg gtgtcaattt cgcttttgcc agatagtcag aaacaacttt cgagcctggt
    39901 gccagcgacg ctttgaccca cggttgccgc ttgaggccca gagttacagc ttttttcgcc
    39961 agcaagcctg cggccatcag cacacttggg ttagaggtgt tggtgcacga ggttatcgca
    40021 gcaatgacca ccgcgccatc aggtaactga tactgatgtc cgttcataac ataatcgacc
    40081 ggctggcgat ctttatgcgt ggcattcact tccagttcgt tactggcggc aaatgctttt
    40141 ggtacatcgg gcagtgcaac gcgatcctgt gggcgtttag gccctgccag gctcgcttca
    40201 acgtcattca tatccagttc taacgtactg gtaaaaattg gttcatcgcc cgggttacgc
    40261 cacatgccct gcgctttggc atatttttcg accaactcga cctgatcttc gctgcgcccg
    40321 cttaaacgca tgtaatcgag ggtgacagca tcgattggga agaagccaca ggtggcacca
    40381 tattctggcg acatattggc aatggtggcg cgatccgcca gcggtagtga atccagccca
    40441 tcaccataaa attcgacgaa tttccccacc acgccatgtt tgcgcagcat ttgggtaacg
    40501 gtgagaacca ggtctgtggc ggtaatacct tcacgtaatt ttccggtgag tttaaagccc
    40561 actacgtccg ggataagcat ggaaaccggc tggcctaaca ttgcggcttc ggcttcgatg
    40621 ccaccaacgc cccaccccag cacgccaagg ccgttgatca tggtggtgtg cgagtcagta
    40681 ccaacgagtg tatccggata agcaatccat tcaccgtcct gcaattcgct ccacactgct
    40741 ttgccgagat attcgaggtt aacctgatgg caaatgcctg tgcctggcgg cacgacgcta
    40801 aaacgactga acgcctgctt tccccatttc aggaacacat aacgttcgtg gttgcgctcc
    40861 atttccaggc gtacgttttc ttcaaatgcc tcatcatcac caaaacgatc gacggtcacc
    40921 gagtggtcaa tgaccaggtc gaccggtgag agcgggttaa cctttgcagt atcgccaccg
    40981 aggcgtttaa ccgcttcgcg cattgccgcc agatcaacaa cggcaggtac gccggtaaag
    41041 tcctgcatca gcacccttgc cgggcggtag gcaatttcac ggtcagcatg ggcatttttc
    41101 agccatcccg ccagcgcgtg gatatcctct tcggtaaccg agttaccatc ctgccagcgc
    41161 agcaggtttt cgagcaaaac tttgagtgac ttaggcagac gggtgatatt gcccagtgat
    41221 ttggcagcaa gcggcaggct gtagtagtgg taagttttat ctttggcctg caacgtgtcc
    41281 ttactggctt ctcgtagggt tgacgacata gctcctcctt aatgacaggg ttgcgaagcc
    41341 ctgattctct tcagggtgaa tattaaagat aacacaaaca tcgcgtaacg atttgataac
    41401 aacccaaatt gataaaagag gagaaacctt gagacaaagc aaacagcggg gtcagggatc
    41461 agtgagtaat gatccagagg agttcggccc agaagagcat cgacagagaa aatgcgccca
    41521 tccaggacca atatttcata tttgtgttga tcataggcta cacctttaac ggttattact
    41581 ttgtgttgag ataactcaac ctgttattgc tgcggtgtat taaaagccct ttaattcatt
    41641 aaaaatgggt aataagaatg atgataatta ttgtttctgc tgatcatctt cagcaaacag
    41701 ttctataaag gcttgctgct gcgatgttag ttgccagtcg tctggaataa cgacggaaca
    41761 ttcccgtttc gtttctgttt cattgtcact atgctgacat aatggtgacg actccagttg
    41821 ctcctgaccg accatagtta tccccacact ttccaaatca gcagtgcgac aaccacccag
    41881 aaaagagcag aaccaagaaa taccgccagc caggctttgc gttttaattc agggtcccgg
    41941 cggggttcct gatttccaga aggcattgct aacctcatat aatcgccatc acttatcagc
    42001 aagacgttga acgattggtg taattaatca tacgatgaga caaatcctaa agaaacttta
    42061 gtcaaaaatc cagcgaattt acgaatcgtc tccagtgaat aattcaatct tttgacctga
    42121 aatagatatt tgaaaactcg agtttgcgag gcgggtaatt agacactact ttacaggtaa
    42181 tttaccctat aaatatgagg gataaaaata ttgtgcacgg tatacaaata agcaacaaaa
    42241 gagaatatat tccggcacct tcgctacata aaaggtgccg aaaataacgc aagaaattat
    42301 ttttccggca gttttatatc tttaaacatg atctcaattt cttcattaga gcgtaatgcg
    42361 acagccgcat caacgacatc acgcgttaaa tgcggtgcaa aacgctgaat gaaatcatac
    42421 atataactgc gcaaaaaagt actacggcga aaaccaattt tggttgtact gtggctgaag
    42481 atatcgtgag catcaacgcg caccaggtcg ggatcggcga ccggatccac cgccatgctg
    42541 gcaatgaccc ctacccccag ccctaaccgg acgtaagttt taatgacgtc agcatccgtt
    42601 gccgtgaaga cgatacgcgg cgttaaccct gcgcggttaa aggcagtatc cagctctgaa
    42661 cgtccggtaa agccgaaggt atatgtcacc aacggatatt gcgccagttc ttcaatggta
    42721 atggcttttt tgcctgccag cgggtgatcc ggagtgacta caatagcccg attccagtgg
    42781 tagcacggta acatcactag atcttcatac agatgcaacg cttctgtggc gatagcgaaa
    42841 tcagcattgc ctttagagac ggcatcagca atttgtgtcg gcgagccctg gtgcatatgc
    42901 aaagaaacgc ggggataacg ctcaataaag cctttgatga cgtttggtaa cgcgtagcgt
    42961 gcctgggtat gcgtggtggc gatatacagc gagcctttat ccggccaggt gtgctctccg
    43021 gcgaccgatt ttatggcatc aactttcgac aggacttcgc gagcgatacg aattatttct
    43081 tgccctgctg gcgttacctg cgtcaggtgc ttgccgcttc gggaaaaaat ttgaatgcct
    43141 agctcgtctt ccagcattct gacttgttta ctgatcccgg gttgtgatgt gtaaagtcct
    43201 tccgctgttg atgagacatt caggttatga ttgaccacct caacaatata gcgaagttgt
    43261 tgtaatttca tgttaaacca tccacttaga cgccatcaga acatcgtttt ttcgtcacac
    43321 ctggcgggtg actaatacga tttaataata aaaaaagtgc taactataac cactatatca
    43381 tttatagcct tagtgtatag gtgtgaagaa aaaataatat gcaataaaaa agggccgctt
    43441 tcgcgaccct ttgtttataa aaacctgaca gaattaaagg ttattttttc ccttcaaccc
    43501 atttgccatc aacataaaat gctgaccagc cagtcgcctt tccgtctttt tccgaagaga
    43561 catattgctg tttggtttta cggctaaagc gaaccatggt cttattacct tccggatcct
    43621 gctgtggcgc atcggccaga taacgcagtt tttccggcag acggtcgcgg aagcgataaa
    43681 gctcttccac cagtggcgca cgtgtttcac gcgatttcgg gaaggtattg gccgccagga
    43741 acacaccggc agcaccgtca cgcagcacga aataagcatc tgatttttcg cacggcagct
    43801 ccggtaatgg caccggatct tctttcggtg gtgccacttc gccgttacgt aaaatcttac
    43861 gcgtgttttt acactcttcg ttagtgcagg ccatgtattt accgaaacgc cccattttca
    43921 ggtgcatttc agagccacat ttttcacact caacgatcgg gccgtcataa cctttaatgc
    43981 ggaattcgcc ctcttcgatc tcgtaaccgt cgcaggttgg gttattacca cagacatgca
    44041 acttacgttt cggatcgatg agatagctgt ccatcgccgt gccgcatttc gggcaacgac
    44101 gttttgcgcg cagcgcgttg gtttcagcat cttcgccttc cagcacgttc agcacttcgt
    44161 tttccggcac caggttgatg gtggttttgc aacgctcttt cggtggcaga gcgtagccgg
    44221 aacagccaag gaataccccg gtgctcgctg tgcgaatccc cattttgcga ccacaggtcg
    44281 ggcagtcgat gctggtcaga accatctggt tcgggcgcat ccccccctct tccggatctt
    44341 tttcagcttt atctaactgc tgggtgaaat ccgagaagaa gtgatccagt acagctttcc
    44401 actctgcttc gtgatttgcc acctggtcaa ggctgttttc catctgcgcg gtaaagtcgt
    44461 agttcattaa ctcgcggaaa ttttcttcaa ggcgatcggt gacgatttcg cccatttttt
    44521 ccgcatagaa acgacggttt tctacacgca catagccacg atcctgaatg gtcgaaatga
    44581 tcgacgcata ggtagacgga cgaccgatac cgcgtttttc cagttcttta accagcgatg
    44641 cttcactgaa acgggctggc ggtttggtaa agtgctgggc tggtgtcagt tcaacgagcg
    44701 tcagagcatc gcctttatcg actgccggta agatacgatc ttcatcgcct ttacgcagtg
    44761 caggcatcac ttttgtccag ccatcaaagc gcaaaatacg accgcgtgct ttcaggcgga
    44821 aatcgcccgc accaacggtc agcgtcgtgg agtcatattt cgctggggtc atctggcagg
    44881 caacgaactg acgccagatt aactggtaca atttttgcgc atctgcttcc atatccttca
    44941 acgattccgc catcacattg acgtcagaag gacgaatcgc ttcgtgcgct tcctgtgagt
    45001 tttctttgct ggcgtactga ttcggacttt ccggcagata tttcttacca aaattatcgc
    45061 tgatataacc gcgaaccata tttaccgcgt cctgactcag gttagtggag tcggtacgca
    45121 tgtaagtgat atagcctgct tcatacaaac gctgcgccat catcatggtt tttttcacgc
    45181 caaatccaag acgggtgctg gcagcttgtt gcagcgtaga ggtaataaaa ggagcgccag
    45241 gtttactggt tgtcggtttg tcttcacgtt ccagcacgct gtagcgcgct ttttccagca
    45301 gacttaccgc agcctgagtt tgttctttgt tgaccggacg gaacggtttg tcgttctgat
    45361 gagtcacctg caacgccaac gcttcaccag atggcgtggt cgtgctggca tcgacttccc
    45421 agaactcttc cggtacgaac gctttaattt cacgctcacg ctcgaccacc aggcgaaccg
    45481 ccaccgactg cacacgaccg gcagacagac cacgagcgat ctttttccat agaagcggcg
    45541 aaaccatata ccccaccacg cggtccataa agcgacgcgc ctgctgggca ttaacacgat
    45601 caatattcag ctcacccggt ttgttaaatg cctggcggat cgcgttttta gtaatttcgt
    45661 taaacaccac tcggctatag cgcgcatcat caccaccaat cacttcccgc aggtgccatg
    45721 caatggcttc cccttcgcgg tcaaggtcgg ttgcgagata gatgtggtcg gctttttcag
    45781 ccagttgttt cagttcagag acgaccttct ctttaccagg caacacttca tagtgcgcct
    45841 cccaattgtg ccacgggtca acccccatac ggttgacgag agcgccacgt tcatcctttt
    45901 taggcttttt agccgtcttg gtggaggtag agtcggcact ctttttggca gctgagccac
    45961 tggtcggcaa atcgcggatg tgaccgacgc tggatttcac cacgtagtca ctacccagat
    46021 acttgttgat cgttttggct tttgccgggg actcaacgat gacaagagct ttacccatat
    46081 tcaccttacc taatttgatt cttccaggaa tgcgtcgcac gttgataaac cttccactgg
    46141 caacgagtta ccgatatgga ctcgctctct gcggatatca accccttgtc gtgactctgt
    46201 tcacgaaaat tcaggtgact gagttaacag gtcttggcct acaggcgcta tagcacgacg
    46261 gaattttggt cgaatgtcaa gcaaatctat tgccagattg acgaaagcgt cacactgtac
    46321 ctgataaaat ccgatacgca actttattag catgcaaaat ccaaagttgc cagtaaggcg
    46381 ctcttgccac cagcgtggta tatttgtctg caagccatgc ctggcgtaga ctaatgtcct
    46441 tgtttaagga gtaaataatg aacaaagaga cacaacccat cgaccgcgag acgctgttga
    46501 aagaggccaa caaaatcatt cgtgagcatg aggatacgct cgcagggatt gaagccaccg
    46561 gcgtcacgca gcgtaatggc gtgctggtat ttaccggcga ctacttttta gatgaacaag
    46621 gattgccaac agccaaaagc accgcggtgt ttaatatgtt caaacacctg gcccatgtgc
    46681 tttcagaaaa atatcacctg gttga
//
LOCUS       DAZGQL010000028        46035 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.028, whole genome shotgun sequence.
ACCESSION   DAZGQL010000028 DAZGQL010000000
VERSION     DAZGQL010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 46035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46035
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.028"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(475..1323)
                     /locus_tag="ACHKIR_001837"
     CDS             complement(475..1323)
                     /locus_tag="ACHKIR_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFO9989586.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVAMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            1532..2167
                     /gene="yfhb"
                     /locus_tag="ACHKIR_001838"
     CDS             1532..2167
                     /gene="yfhb"
                     /locus_tag="ACHKIR_001838"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HFO9989587.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHNEARLQALQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            2225..2728
                     /gene="tadA"
                     /locus_tag="ACHKIR_001839"
     CDS             2225..2728
                     /gene="tadA"
                     /locus_tag="ACHKIR_001839"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFO9989588.1"
                     /translation="MSEVEFSHEYWMRHALTLAKRAWDEREVPVGAVLVHNNRVIGEG
                     WNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRV
                     VFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQK
                     KAQSSTD"
     gene            complement(2725..4281)
                     /gene="mltF"
                     /locus_tag="ACHKIR_001840"
     CDS             complement(2725..4281)
                     /gene="mltF"
                     /locus_tag="ACHKIR_001840"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HFO9989589.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            4539..8426
                     /gene="purL"
                     /locus_tag="ACHKIR_001841"
                     /gene_synonym="purI"
     CDS             4539..8426
                     /gene="purL"
                     /locus_tag="ACHKIR_001841"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HFO9989590.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHTIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYVEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWVIDGEQQPKSLFKMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGASGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTREVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVEAVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLAGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            8983..10410
                     /gene="qseE"
                     /locus_tag="ACHKIR_001842"
     CDS             8983..10410
                     /gene="qseE"
                     /locus_tag="ACHKIR_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HFO9989591.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSS
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIDLPSSKNTK"
     gene            10575..11288
                     /gene="qseG"
                     /locus_tag="ACHKIR_001843"
     CDS             10575..11288
                     /gene="qseG"
                     /locus_tag="ACHKIR_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HFO9989592.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQSHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGNWQ
                     NTFKQGILLADAKITPYERRQLVARIDALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTDEVTPDEP"
     gene            11278..12612
                     /gene="glrR"
                     /locus_tag="ACHKIR_001844"
     CDS             11278..12612
                     /gene="glrR"
                     /locus_tag="ACHKIR_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HFO9989593.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDIDVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            12673..13011
                     /gene="glnB"
                     /locus_tag="ACHKIR_001845"
     CDS             12673..13011
                     /gene="glnB"
                     /locus_tag="ACHKIR_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HFO9989594.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(13056..14246)
                     /gene="hmpA"
                     /locus_tag="ACHKIR_001846"
     CDS             complement(13056..14246)
                     /gene="hmpA"
                     /locus_tag="ACHKIR_001846"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008941 - nitric oxide dioxygenase NAD(P)H
                     activity [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0051409 - response to nitrosative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HFO9989595.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGLSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFAAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            14575..15828
                     /gene="glyA"
                     /locus_tag="ACHKIR_001847"
     CDS             14575..15828
                     /gene="glyA"
                     /locus_tag="ACHKIR_001847"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFO9989596.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDASGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            complement(15891..17084)
                     /locus_tag="ACHKIR_001848"
     CDS             complement(15891..17084)
                     /locus_tag="ACHKIR_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFO9989597.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDAPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVMVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGCLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVNTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGEHWLNTIIRQTGFNPFDRDEGPSVKATKIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            17202..20483
                     /locus_tag="ACHKIR_001849"
     CDS             17202..20483
                     /locus_tag="ACHKIR_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HFO9989598.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKSWQAVWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPA
                     LVGLLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMT
                     GFTLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKR
                     AVSAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLH
                     VANHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEE
                     KRFEQYFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLKGYRLAIREIGKCN
                     ALLDDAVALTPATAIQGVLHGINPERLTIELSDADGNITLSYQEHQPQELPLPDVAKA
                     PLAAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNR
                     ADFPQAVAYATQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGN
                     SKAGGYYGLARLAARNGHFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARL
                     QREKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTL
                     AAEMLNALDCQRTLPLYLQASLLPKAERGELVTKAIDVFPQFVRFPNTLEEVAALESI
                     EECWFARHLLACFYYNKCSYNKAITLWQRCVEMSPEFADGWRGLAIHAWNKQHDYELA
                     ARYLDNAYQLAPQDARLLFERDLLDKLSGVTPEKRLARLENNLEIALKRDDMTAELLN
                     LWHLTGQADKAADILATRKFHPWEGGEGKITSQFILNQLLRAWQHLDAREPQQASELL
                     HAALHYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEATRCLRLAATGDRTINIHS
                     YYNDQPVDYLFWQGMALRLLGEQHTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLL
                     SLYGDLQQQHKEKCLMVAMLASAGLGEVAHYESARAELMAINPAWPKAALFTTVMPFI
                     FSYVH"
     gene            20580..21563
                     /locus_tag="ACHKIR_001850"
     CDS             20580..21563
                     /locus_tag="ACHKIR_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFO9989599.1"
                     /translation="MPKKMTRTRNLLLMATLLGSALFARAADKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            21586..23097
                     /locus_tag="ACHKIR_001851"
     CDS             21586..23097
                     /locus_tag="ACHKIR_001851"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFO9989600.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVSPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKDAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHAPVNVDELMSAILSVH"
     gene            23122..24120
                     /locus_tag="ACHKIR_001852"
     CDS             23122..24120
                     /locus_tag="ACHKIR_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO9989601.1"
                     /translation="MSASSLLLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAVACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            24153..25247
                     /locus_tag="ACHKIR_001853"
     CDS             24153..25247
                     /locus_tag="ACHKIR_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFO9989602.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGEGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            25259..26131
                     /locus_tag="ACHKIR_001854"
     CDS             25259..26131
                     /locus_tag="ACHKIR_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFO9989603.1"
                     /translation="MTIYTLSHGSLKLGVSDQGGVIEGFWRDTTPLLRPGKKNGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGQWQCVSRNDDILC
                     LVYEHRSGVYHYRVSQTFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIIETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVALL"
     gene            complement(26280..26702)
                     /locus_tag="ACHKIR_001855"
     CDS             complement(26280..26702)
                     /locus_tag="ACHKIR_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFO9989604.1"
                     /translation="MNSLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(26799..28001)
                     /gene="hcaD"
                     /locus_tag="ACHKIR_001856"
     CDS             complement(26799..28001)
                     /gene="hcaD"
                     /locus_tag="ACHKIR_001856"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HFO9989605.1"
                     /translation="MKEKTIIIVGGGQAAAMAAALLRQQGFSGELHLFSDEQHLPYER
                     PPLSKSMLLEDSPQLQSVLPAHWWQENNVHLHSGVTIKTLGRDTQELVLANGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRGCKVTVIELAASVMGRNAPPPVQRYLLPRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLRADVVIYGIGISANDQLAREANLDTANGIVIDEACRTCDPAIFAGGDV
                     AITRIDNGALHRCESWENANNQAQIAAAAMLGLPLPRLPPPWFWSDQYSDNLQFIGDM
                     HGDDWICRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRLIRKWIQSGKTFDAKLLID
                     EDIALKSL"
     gene            complement(28011..28823)
                     /gene="hcaB"
                     /locus_tag="ACHKIR_001857"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             complement(28011..28823)
                     /gene="hcaB"
                     /locus_tag="ACHKIR_001857"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HFO9989606.1"
                     /translation="MSDLHNESIFITGGGSGLGLALAERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVNQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            complement(28820..29140)
                     /locus_tag="ACHKIR_001858"
     CDS             complement(28820..29140)
                     /locus_tag="ACHKIR_001858"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HFO9989607.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            complement(29140..29658)
                     /gene="hcaF"
                     /locus_tag="ACHKIR_001859"
     CDS             complement(29140..29658)
                     /gene="hcaF"
                     /locus_tag="ACHKIR_001859"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HFO9989608.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QVSETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDGNWRLLERDIVLDQ
                     AVISSHNLSVLF"
     gene            complement(29655..31016)
                     /gene="hcaE"
                     /locus_tag="ACHKIR_001860"
     CDS             complement(29655..31016)
                     /gene="hcaE"
                     /locus_tag="ACHKIR_001860"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit alpha"
                     /protein_id="HFO9989609.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKHGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            31152..32042
                     /gene="hcaR"
                     /locus_tag="ACHKIR_001861"
     CDS             31152..32042
                     /gene="hcaR"
                     /locus_tag="ACHKIR_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HFO9989610.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTEQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPAIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            32202..33341
                     /locus_tag="ACHKIR_001862"
     CDS             32202..33341
                     /locus_tag="ACHKIR_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HFO9989611.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSSLTGKLVTMFDYRVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(33333..34613)
                     /gene="csiE"
                     /locus_tag="ACHKIR_001863"
     CDS             complement(33333..34613)
                     /gene="csiE"
                     /locus_tag="ACHKIR_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HFO9989612.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATTATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSHFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFETRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRKSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRSAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(34804..35658)
                     /locus_tag="ACHKIR_001864"
     CDS             complement(34804..35658)
                     /locus_tag="ACHKIR_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417029.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO9989613.1"
                     /translation="MALPVNKRVLKILFILFVVAFCIYLIPRIAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLHGWFIPSATGPAEKAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILDVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            complement(35803..36606)
                     /gene="suhB"
                     /locus_tag="ACHKIR_001865"
     CDS             complement(35803..36606)
                     /gene="suhB"
                     /locus_tag="ACHKIR_001865"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0046854 - phosphatidylinositol phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HFO9989614.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            36725..37465
                     /gene="trmJ"
                     /locus_tag="ACHKIR_001866"
     CDS             36725..37465
                     /gene="trmJ"
                     /locus_tag="ACHKIR_001866"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HFO9989615.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            37735..38223
                     /gene="iscR"
                     /locus_tag="ACHKIR_001867"
     CDS             37735..38223
                     /gene="iscR"
                     /locus_tag="ACHKIR_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HFO9989616.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            38335..39549
                     /gene="iscS"
                     /locus_tag="ACHKIR_001868"
     CDS             38335..39549
                     /gene="iscS"
                     /locus_tag="ACHKIR_001868"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFO9989617.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            39577..39963
                     /gene="iscU"
                     /locus_tag="ACHKIR_001869"
     CDS             39577..39963
                     /gene="iscU"
                     /locus_tag="ACHKIR_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HFO9989618.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            39980..40303
                     /gene="iscA"
                     /locus_tag="ACHKIR_001870"
     CDS             39980..40303
                     /gene="iscA"
                     /locus_tag="ACHKIR_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HFO9989619.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            40399..40914
                     /gene="hscB"
                     /locus_tag="ACHKIR_001871"
     CDS             40399..40914
                     /gene="hscB"
                     /locus_tag="ACHKIR_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HFO9989620.1"
                     /translation="MDYFTLFGLPACYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNETWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            40931..42781
                     /gene="hscA"
                     /locus_tag="ACHKIR_001872"
     CDS             40931..42781
                     /gene="hscA"
                     /locus_tag="ACHKIR_001872"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HFO9989621.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSTAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            42783..43118
                     /gene="fdx"
                     /locus_tag="ACHKIR_001873"
     CDS             42783..43118
                     /gene="fdx"
                     /locus_tag="ACHKIR_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HFO9989622.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDVALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            43130..43330
                     /gene="iscX"
                     /locus_tag="ACHKIR_001874"
     CDS             43130..43330
                     /gene="iscX"
                     /locus_tag="ACHKIR_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HFO9989623.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            43389..44672
                     /gene="pepB"
                     /locus_tag="ACHKIR_001875"
     CDS             43389..44672
                     /gene="pepB"
                     /locus_tag="ACHKIR_001875"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HFO9989624.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGTDDLGLI
                     QRAARKIDGLGIKHVQLSGEDWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLRDQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPELIIDAATLTGAAKTALGNDYHALFSFDDALAARLLAS
                     AAQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            44791..45567
                     /gene="sseB"
                     /locus_tag="ACHKIR_001876"
     CDS             44791..45567
                     /gene="sseB"
                     /locus_tag="ACHKIR_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HFO9989625.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEVLEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQAAGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(45599..45724)
                     /gene="timP"
                     /locus_tag="ACHKIR_001877"
     CDS             complement(45599..45724)
                     /gene="timP"
                     /locus_tag="ACHKIR_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic inner membrane protein TimP"
                     /protein_id="HFO9989626.1"
                     /translation="MKIRCFCIVLIVSGALLSEVNNNRSLSGNNLLAVNNLQSSK"
BASE COUNT        10837 a        11632 c        12699 g        10867 t
ORIGIN      
        1 cctggcagtg cgattaaagg agcagaggga ataaaaaacg ggggcatgac caacccccgg
       61 tacaaggaag gagtaactac aatggaaaaa ttaatgaaga cctttagtgc tacgttatat
      121 ggcgaataaa gactgccaca gggaagtttg cagaaacagt gatgccgatc gtgtttctga
      181 cgctcttctc tgaataagcg taatttaagt gaggtctgaa agtggatggc tgctcagtag
      241 aaaatgctaa agaaaggggg aggctttgtg ttgctgaact tatgcaaaat tttttttgca
      301 ccataaatcc aacaacacat tcaataacac attactgtat cactatgaaa ataaatgtgt
      361 ttttatatga tttcacacat atcacaattg atgcattttt tagtgattaa cagcgccata
      421 aggaaactct caaaacaaca caaaccgggc gggcattata cgcgcattct ttactcagac
      481 cagttttttc accagcgctt cactgtgacg aatacgttct ggagccagtt ccagatcctg
      541 ctgaatcagc gcaatgaaca gcaaatccgt taacattccc tgcgcgtgac aagcagagat
      601 tgaagcactg tttgtcgctt gttcttcggc aatggtatac aggcaatgag aagcacgctg
      661 ttgcagggca ttgggagtaa agccggtaat cgccagcact tttccgccca ctcgcagcat
      721 ctcatctgcc gccaggttta actcgcgtcg tacaccggtg taggaaatgg ctaataacag
      781 atcgtcaggg gacgacgcct gtactgtagc gagtagcgca tgcatatcgc gcactgcggc
      841 agcattgaag ccaatcttca tcagcttcca ggcaaagttt tgcgccacca gacccgaagc
      901 gccaataccg gtcagaatta tccgccgcgc agagcgcaac attgccacgc attcatgcag
      961 tttctcttca ctattaacgt ttaacgttgc gtacatagcg gcagtatttt ctttaatcag
     1021 tttttcgccg accagccgta acggatcatc accgcggatt tggttatgaa tgggcacgga
     1081 gggagattcc ggctggcttg ccagcgcttc actcaacgcc aacttaagtg ccggaaaacc
     1141 tttatagccg agtttttgcg cgaacttcac gacgctggac tgactgactc cggcttcgtt
     1201 ggccagttgc tgagagctta aatggcgcgc cgtatcaggt tgtagcagca gataatccgc
     1261 cagtttttta tcactttggg caagcccctg gtaacgctga cggatacgaa gtaagccgtt
     1321 catatctttc ctcgcgatga aatagtggcg acaacaggca atatgagaca cacgcgagtg
     1381 aattttatat tccattgccg ctaattgcca tgaattaaaa attccagtta gccatgatgc
     1441 ggtcgattgt tgccggaaaa gtgatgatct atctgttagg ctatgtagta gcgaaaatta
     1501 atcaggatgt ttcagtccag aggagtatgg tttggcaact cacgagcgtc gtgtggtgtt
     1561 ttttgactta gatggaacat tgcatcagca ggatatgttc ggcagttttc tgcgttattt
     1621 actgcgtcgc caaccgctga atgcgttact tgtcctgccg ttgttgccga tcatagccat
     1681 tgcgttattg ataaaaggtc gtgcggcacg ctggccgatg agtctgcttc tgtggggatg
     1741 cacttttggt cacaatgaag cgcgtttgca ggcgttgcag gccgatttcg tacgctggtt
     1801 tcgtgacaat gttaccgcct ttccgctggt tcaggagcga ttaaccacct acctgttaag
     1861 ttccgatgct gatatctggt tgattaccgg ctctccgcag ccgctggttg aagcggttta
     1921 tttcgatacg ccctggctgc cgcgggttaa tcttatcgcc agccaaattc agcgtggcta
     1981 tggtggttgg gtattgacga tgcgttgtct gggacatgaa aaggtcgcac aactggagcg
     2041 caaaatcggc acgccgctgc ggctgtacag tggctatagc gacagtaatc aggacaatcc
     2101 gctgctttat ttctgtcagc atcgttggcg agtaaccccg cgcggtgaac tccagcaact
     2161 ggaatagagt aaagcatagc gtccgtgtat aatgcgccgc gcttttataa ccggagtttt
     2221 ctttttgtct gaagtcgaat ttagccacga atactggatg cgtcacgcgc tgacgctggc
     2281 aaagcgtgcc tgggatgagc gggaagtgcc ggtcggcgcg gtattagtgc ataacaatcg
     2341 tgttattggc gaaggctgga accgtccgat tggtcgacat gatcccaccg cacatgcaga
     2401 aatcatggcc ctgcggcagg gcggtctggt gatgcaaaat tatcgactga tcgacgccac
     2461 gctgtatgtc acgcttgaac cctgtgtaat gtgtgccgga gcaatgatcc acagtcgcat
     2521 tggtcgcgtg gtctttggtg cgcgtgacgc gaaaactggc gctgcgggat ctttaatgga
     2581 tgtgctgcat catccgggta tgaatcaccg ggtggaaatt accgaaggaa tactggcgga
     2641 tgagtgcgcg gcgttgctca gtgacttctt tcgcatgcgc cgccaggaaa ttaaagcgca
     2701 gaaaaaagcg caatcctcga cagattaatt ttgtttctct tcactccctt ttctggaaaa
     2761 cagcagagag ggagagtggg tcaaatagtt tgatgacgac tgggaaagaa acgagagaaa
     2821 aggaaatttc tctttgccca gctccgtagg cgataccgcc ggataatcct gcgccagttg
     2881 catcgccgct tctgtagcct gcttctcttt ctcctgcaga taacccacca ggctaatctg
     2941 atacttacga atattttcga cataagcgta ggcttcatgc ccacgagcgt agccgtaagt
     3001 cagcttgctg taatagggtt tctggctaag taaaggcaga cgctgtttta cgtcagccca
     3061 actgtcagga ttacctttgg tttttgccgt cagggcgcgg gcatccagca tatgcgcata
     3121 gcccatattg tacgcggcaa gggcaaacca gatccgttcg ttctccggca cactttccgg
     3181 cactttactc atcatatcct gcaaataacg cacgccgccg ctgatgctct gttcggcatc
     3241 ggtacgatcc gtaatgccga ggctttgcgc ggtgtttttg gttaacatca tcatgccgcg
     3301 cacacccgtc ggtgaagtgg cctgagcatc ccagtgcgat tcttgataag caatagcggc
     3361 cagcaaacgc cagtcaattt cttcggcgta tttctcaaac aggggcttta actgcggcag
     3421 taccgcatcg acggcgcgta aaaatgtgcg cgtatcgacg taatcaaaat catcgccatg
     3481 ccccaggtat ttctcttcaa tgcgtgccag cgtaccgtct tcattcatct cattgaagaa
     3541 atcgagcaga gcggcggaaa gggtattatc gccatctaac gggctaaacc aggtcaccgg
     3601 ttgttcatcg gtgatatcga gcgctacggc gagttccggg tgaacgcgct gaaacaggct
     3661 gatggcgaca gaatcagcaa tggtgtaatc gagttttcct tcgatgacat cttccattaa
     3721 ttccgcagag ccttttttgt cgtctacctt ccagcttaat tccgggaatt ttgtttcttt
     3781 cagggtctgg agatcgttaa ccaccacatg acccggtgca acggtgagtt gctccgccgt
     3841 caggttgccc agtgtacgtg ggcgatactg acccacttta taaaccagtt gttgtgacac
     3901 ggaataatag gtagggccag gctgataatt ttttacacgc tcactgttat agaccaggcc
     3961 tgccgccagc aggtcggcgt taccattatc gaggtcgtca aacagctggc tgatattctg
     4021 ccgcacggtc actttcagtt ttacgccgag gtaatcggca aactgtttcg ccagttcgta
     4081 atccaggcca aaaggtttcc cgttgatttc gttataagtc aggggggtat gaatggtgct
     4141 cacacgcaac tctccccgcg cttgaatggc ggcgatacgg ttgtcggctt taccaaacca
     4201 gggaatggat ggccagagag cgaccgcgag cagcagtgcc agaatgccga tgaacagata
     4261 attaatcttt aattttttca attagttaat tctctgtgtc gtgcgcgtcc cagcttgaaa
     4321 aaacgtaata atagtgaaag gtttactcat aaatgagcgg cattttgcgt aaacctgcgc
     4381 cagttggcaa cttattacag ccattggcgg cacgcgttgc taattcacga tggtgatttt
     4441 atttccacgc aaacggtttc gtcagcgcat cagattcttt ataatgacgc ccgtttcccc
     4501 cccttgggta caccgaaagc ttagaagacg agagacttat gatggaaatt ctgcgtggtt
     4561 cgcctgcatt gtcggcattc cgaatcaaca aactgctggc acgttttcag gctgccaggc
     4621 tcccggttca cactatttac gccgagtatg tccattttgc tgacctcaat gcgccgttaa
     4681 acgatgatga gcacgcacaa cttgaacgcc tgctgaaata tggcccggca ctcgccagcc
     4741 acgccccgca aggcaaactc ctgctggtga ccccgcgtcc tggcaccatc tctccctggt
     4801 cttcgaaagc gaccgatatc gcccataatt gcggtctaca acaggtaaac cgccttgagc
     4861 gcggcgttgc ttactatgta gaagccggta cgctgaccaa tgaacaatgg cagcaggtta
     4921 ccgctgaact gcacgaccgc atgatggaaa cggtcttttt tgctttagat gatgcagagc
     4981 agctgtttgc acaccatcaa ccgactccgg tcaccagcgt tgatttgctg gggcagggcc
     5041 gtcaggcgct aatcgacgct aacctgcgtc ttggcctggc actggcggaa gatgaaattg
     5101 actatttgca ggatgctttc acaaagctgg gtcgtaaccc gaacgacatc gaactgtata
     5161 tgtttgccca ggcgaactcc gagcactgcc gccacaaaat ttttaacgcc gactgggtta
     5221 tcgatggtga acagcagccg aaatcactgt ttaagatgat caaaaacacc ttcgaaacca
     5281 cgccggatca cgttctctct gcttataaag ataacgccgc cgtaatggaa ggttctgaag
     5341 tgggccgcta ctttgctgac cacgaaacgg gccgctacga tttccatcag gaacctgcgc
     5401 atatcctgat gaaagttgaa actcacaacc acccgacggc gatttctccg tggccgggcg
     5461 cggcgaccgg ttccggcggt gaaatccgcg atgaaggtgc ctccgggcgc ggcgcgaagc
     5521 cgaaagctgg tctggtcggt ttctccgtgt ccaacctgcg aattcctggc ttcgaacagc
     5581 cgtgggaaga agatttcggt aaacctgagc gcattgttac cgcgctggac atcatgaccg
     5641 aaggcccgct gggcggtgcg gcgtttaaca acgaatttgg tcgtccggca ctgaatggct
     5701 acttccgtac ttatgaagaa aaagtgaaca gccacaatgg cgaagagctg cgcggttatc
     5761 acaaaccgat catgctggcg ggcgggatcg gcaacattcg tgccgatcac gtacaaaaag
     5821 gcgagatcaa cgttggtgcg aagctggtcg ttctcggcgg cccggcaatg aacatcggtc
     5881 ttggcggcgg cgcggcgtct tccatggcgt ctggtcagtc tgatgccgac ctcgacttcg
     5941 cttccgtaca gcgcgacaac ccggagatgg agcgtcgctg ccaggaagtg atcgaccgtt
     6001 gctggcagct tggtgatgcc aacccaatcc tgtttatcca cgacgttggc gcaggcggtc
     6061 tttctaacgc catgccggaa ctggtgagcg acggcgggcg cggcggtaaa ttcgaactgc
     6121 gcgatatcct gagcgacgaa ccgggcatga gcccgctgga aatctggtgt aacgaatccc
     6181 aggaacgcta cgtgctggca gttgctgccg atcagttacc gctgtttgac gaactgtgta
     6241 agcgtgagcg cgcgccatac gcggtgattg gtgaagcgac cgaagaactg catctttctt
     6301 tgcacgatcg tcattttgat aatcagccga tcgatctgcc gctggacgta ctgcttggta
     6361 aaacgccgaa gatgacccgc gaagtacaaa cgctgaaagc gaagggtgat gcgctggttc
     6421 gtgaagggat taccattgct gacgcggtga aacgcgtgct gcacctgccg accgtggcgg
     6481 agaaaacttt cctggtgacc attggtgacc gcagcgtaac cggcatggtg gcgcgcgatc
     6541 agatggtggg tccgtggcag gtgccggtcg ctaactgcgc ggtcactacc gccagcctcg
     6601 atagctacta cggtgaagcg atggcgattg gcgagcgtgc gccggttgcg ctgctggatt
     6661 tcgccgcttc tgcccgtctg gcggtcggtg aagcgttaac caacatcgcc gcaacacaaa
     6721 ttggcgatat caaacgcatt aaactttccg ccaactggat ggcggcggca ggccaccctg
     6781 gtgaagatgc aggcctgtat gaagccgtta aagccgtggg tgaagaactt tgtccggcgc
     6841 tgggcctgac gatcccggtg ggtaaagact ctatgtcgat gaaaacccgt tggcaggaag
     6901 gtaacgaaga gcgcgaaatg acgtcgccgc tgtcgctggt gatttctgca tttgcccgcg
     6961 tggaagatgt acgtcacacc atcacgccac agctttctac cgaagataac gcactgctgc
     7021 tgattgattt gggcaaaggc aataacgcgc tgggcgcaac ggcgctggcg caggtttatc
     7081 gtcagcttgg cgacaaaccg gcagatgtac gcgatgtcgc gcaactgaaa ggcttctatg
     7141 acgcgattca ggcgctggtt gcacagcgta agctgctggc gtatcacgac cgctctgatg
     7201 gcggcctgct ggtaacgctg gcggaaatgg cctttgctgg tcattgcggc attgacgcgg
     7261 atatcgcctc tctgggtgac gatcgcctgg cggcgttgtt taacgaagaa ctgggtgcgg
     7321 tgattcaggt tcgtgccgct gaccgtgaag ccgttgaagc ggtactggca cagcatgggc
     7381 ttgcggattg cgtccattat gtagggcagg cggtttccgg tgaccgtttt gtgattaccg
     7441 ccaacgggca gactgtattc agcgaaagcc gcactacgtt gcgtgtctgg tgggcagaaa
     7501 ctacctggca gatgcagcgc ctgcgtgaca acccggagtg tgccgatcag gagcaccagg
     7561 cgaaatcaaa cgacgccgat ccgggcctga atgtgaaact gtcgttcgat atcaacgaag
     7621 atgtggcagc accgtatatt gccactggtg cgcgtccgaa agttgccgtg ctgcgtgagc
     7681 agggggtgaa ctcgcatgtt gaaatggcgg cagctttcca ccgtgcaggc ttcgatgcaa
     7741 tcgatgtgca tatgagcgac ctgctggctg gacgcacggg cctggaggat ttccacgctc
     7801 tggtagcgtg cggtggattc tcctacggtg acgtgctggg tgctggtgaa ggctgggcga
     7861 agtctatcct gttcaatgac cgtgtacgcg atgagtttgc aaccttcttc caccgtccgc
     7921 aaacgctggc gctgggggta tgtaacggtt gccagatgat gtctaaccta cgcgaactga
     7981 tcccaggtag tgagttgtgg ccacgttttg tgcgcaatac ctccgatcgc tttgaagcgc
     8041 gtttcagcct ggttgaagta acccaaagcc cgtctctgct gttgcagggg atggtgggct
     8101 cgcaaatgcc gattgctgtc tctcatggcg aagggcgcgt ggaagtgcgt gatgcggcgc
     8161 atctggcggc actggaaagc aaagggctgg tggcactgcg ctatgtcgat aacttcggca
     8221 aagtcactga aacctacccg gctaacccga acggttctcc gaatggtatt acggcagtca
     8281 cgactgaaag tggtcgagtc accattatga tgccgcaccc ggaacgtgtt ttccgtactg
     8341 tcagcaactc ctggcatccg gaaaactggg gcgaggatgg gccgtggatg cgtattttcc
     8401 gcaatgcacg taagcagttg gggtaagccg tcaacccatt ggttttgcag ccggatgagg
     8461 cattaagcac caactactat tataagctcc caacttcggg agcttttttg tgtctgtaaa
     8521 tcacgacaat gggtggttta ccgcgtcgct ttctggcgac acttaactaa ttgattttaa
     8581 tggcatctaa taagtttatc ttaagttgtc tctttttagc gacacagtgg ctgatttaca
     8641 gccaaatagc gaagaagcca taaccccttc aaaacagcgt acaatcagta cgttaccaaa
     8701 ctattttctt tattggcaca gttactgcat aatagtaacc agtggctcat tcaccgactt
     8761 atgtcagccc cttcgggacg tgctacataa aatacgaatg acgcacaaca aggtgcctgc
     8821 cgtccaactt ctgatatcag cgtagctata tcaaccatcg ggcgaaacgt cgagttaggc
     8881 accgccttat tccataacaa agccgggtaa ttcccggctt tgttgtatct gaacttcccc
     8941 tcggttagca tcaggctatt cgcgtctgac gagagtaaca ccttgaaacg ctggcccgtt
     9001 tttccccgct cattacgaca actggtaatg ctggcatttt tgctgattct gctgcccctg
     9061 ttggtgctgg catggcaagc ctggcaaagc ctgaatgcgc ttagcgatca ggcggcgctg
     9121 gttaaccgca ctacgcttat tgatgcccgg cgtagtgaag cgatgaccaa cgcggcgctg
     9181 gagatggagc gtagctaccg tcagtattgc gtgctggacg accctacgct tgcgaaggtt
     9241 tatcaaagcc agcgcaagcg ttacagcgaa atgctcgatg cccacgcagg cgtactgcca
     9301 gacgataaac tttaccaggc attgcgtcag gatttgaaca atctggccca acttcagtgt
     9361 aacaacagtg gtcccgatgc tgccgccgcc gcgcgtcttg aagcctttgc cagtgccaat
     9421 accgaaatgg tgcaggccac gcgcacagtg gtgttctctc gtgggcagca acttcagcgt
     9481 gaaatcgccg aacgtgggca atattttggt tggcaatcgc tggtgctatt tctggtgagt
     9541 ctggtgatgg tgctactttt cacgcggatg attatcgggc cggtgaaaaa tatcgagcgc
     9601 atgatcaacc gtctggggga agggcgttct ctgggcaata gcgtttcgtt cagtggaccg
     9661 agcgagttac gctcggttgg gcaacgtatt ctttggttaa gtgagcgcct gtcatggctg
     9721 gaatcccaac gccatcaatt tttaagacat ttatctcatg aattaaaaac gccactggcg
     9781 agtatgcgcg agggcactga attactggct gaccaggttg tcgggccgct tacgccagag
     9841 caaaaagagg tggtgagcat tcttgatagc agcagccgca atttgcaaaa actgatcgaa
     9901 caactgcttg attacaaccg taaacaggcg gacagtgcgg tggaactgga gaatgttgaa
     9961 ttagcaccgc tggtggagac agtggtttct gctcatagcc tgcccgcacg ggctaaaatg
    10021 atgcataccg acgtcgatct caaagcaaca gcttgcctgg cggagccaat gctgctgatg
    10081 agcgtactgg ataatcttta ctccaatgcg gtgcactacg gggctgaatc cggtaacatt
    10141 tgccttcgca gcagttcaca tggtgcgcgg gtttatattg atgtcatcaa tacaggcacg
    10201 cccattccgc aagaggaacg cgccatgatc ttcgaaccct tttttcaggg aagccaccag
    10261 cgaaaagggg cggtgaaggg cagcggtctg ggattaagca ttgccaggga ttgtattcgc
    10321 cgtatgcaag gggaactgta tctggtcgac gagagcgggc aagacgtttg tttccgcatt
    10381 gatttaccgt cgtcgaaaaa cacgaaataa tgctaacagc ccatcccatc aggctatttt
    10441 atttgtcagt tcggccccgg gcagtgctcg aaatcttcac gtacgacatg tacgctctgg
    10501 tttctccgcg ctgtccatgt ccaaactgcc tgcaacaatt acgcccactg ggataggctt
    10561 taagtctggt gaatatgcga cacatttttc aacgattatt gccccgacgg ctgtggctgg
    10621 cagggctgcc atgtctggca ttgctgggtt gcgtgcagag tcacaataag ccagccattg
    10681 atacgccagc agaagaaaaa attccggttt atcaactggc tgactatctt tctactgaat
    10741 gtagcgacat ctgggcgctg caaggtaaat caaccgaaac caatccgctt tactggctgc
    10801 gggcgatgga ttgtgctgat cgtttaatgc ctgcgcagtc gcgtcagcaa gcccgccaat
    10861 atgacgacgg caactggcaa aataccttta aacaagggat tttgctcgcc gacgccaaaa
    10921 ttacgccgta tgagcgccgc cagctggtcg cgcgcattga tgcgctaagt accgagatcc
    10981 cggcacaggt tcgccctctg tatcaactct ggcgcgacgg tcaggcgctg caactgcaac
    11041 tggcggaaga gcgccaacgc tacagtaaac tccagcagtc cagcgacagc gagctggata
    11101 ctttgcgcca gcaacatcac gttctacaac agcaactgga actcactacc cgcaagctgg
    11161 aaaacctgac cgatattgaa cgccagctct cgacccgcaa accggctgga aatttctcgc
    11221 cggatacgcc gcacgagagt gaaaagcccg cgccatccac tgatgaggtc actcctgatg
    11281 agccataaac ctgcgcattt attactggtc gatgacgatc cgggattgct gaaactgctt
    11341 ggcctgcgcc tgaccagcga aggctacagc gtggtcacgg cggaaagtgg tgctgaagga
    11401 ttacgggtac tgaatcgcga aaaagtagat ttagttatca gcgacctgcg gatggatgaa
    11461 atggatggta tgcagctgtt tgctgaaatc cagaaagtgc agccgggaat gccggtaatt
    11521 attcttaccg cacatggttc tattcccgat gccgttgctg caacacagca gggagtgttc
    11581 agtttcctca ccaagcctgt cgataaagac gcgctatatc aggcaattga cgatgcgctg
    11641 gaacaatccg cgccagccac cgatgaacgc tggcgcgagg cgattgtcac ccgcagcccg
    11701 ctgatgctgc gtttgctgga acaggcgcgg ctggtggcgc aatcagacgt tagcgttttg
    11761 attaacggtc agagcggcac cgggaaagag attttcgccc aggctatcca caacgccagc
    11821 ccgcgcaata gcaaaccatt tattgctatt aactgtggtg cattgcccga gcaattgctg
    11881 gagtcggagc tgtttggtca tgcgcgtggc gcgtttactg gcgctgtcag caatcgcgaa
    11941 ggtttattcc aggcggcgga aggcggtacg ctatttctcg atgagattgg cgatatgcct
    12001 gcaccgttgc aggtcaaact gctgcgtgtg ttgcaggagc gtaaagtgcg cccgctgggc
    12061 agtaaccgcg atattgatat cgacgtgcgg attatttccg ccactcaccg cgacctgcca
    12121 aaagcgatgg cgcgcgggga attccgtgaa gatctctatt accgcctcaa cgttgtcagc
    12181 ctgaaaattc cggctctggc agagcgcaca gaagacattc cgctactggc aaatcacctg
    12241 ttgcgccagg cggcagagcg acataaaccg tttgtccgcg cgttctccac cgatgcgatg
    12301 aaacgcctga tgaccgctag ctggccgggt aatgtacgcc agttggtcaa cgtgattgaa
    12361 cagtgcgtgg cgctgacctc atctccggtg attagcgatg cattagtaga gcaggcgctg
    12421 gagggcgaaa acacggcgct gccaaccttt gttgaggcac gtaatcagtt tgaactcaac
    12481 tatttgcgta agctgctgca aatcaccaaa ggcaacgtca cccacgcggc gagaatggcg
    12541 gggcgcaacc ggacagaatt ttataaactg ctttcccgac acgagctgga tgcaaacgat
    12601 ttcaaggaat gaattggcgt tatgtgttac gtttagcaga tcaaaagaca ggcgaccttt
    12661 tcaaggaata gcatgaaaaa gattgatgcg attattaaac ccttcaagct ggacgatgtt
    12721 cgcgaagcac tggctgaagt cggcattacc ggcatgacgg tcactgaggt gaaaggtttt
    12781 ggccgccaga aaggccatac cgagctgtac cgcggcgcgg agtatatggt ggattttctg
    12841 ccgaaagtga aaattgagat tgtcgtaccg gacgacattg tcgatacctg tgtcgatacc
    12901 attattcgca cggcgcaaac cggcaaaatc ggtgacggta aaatcttcgt ctttgacgtg
    12961 gcacgggtca ttcgtatccg taccggtgaa gaagacgacg cggcaattta aaccgcgtca
    13021 agggttgccg gatgtttcca tccggcaaca tcaaatcaca gcaccttatg cgggccaaag
    13081 cattcgtaat gaatattttc ctgcttcacg cccagatcca ctaactgttt cgcggcaaac
    13141 tgcatgaagc caaccgggcc gcagagatag aactgcattg tcggatcgct gaacgcacct
    13201 tccagtttgc tcaaatccat cagaccttcg ctatcaaact gacctttagc gcgatcggct
    13261 tcgctcggct gacgatacca ggtgtgcgcg gtaaagcgcg gcagtgacag ccccagttcc
    13321 ttaacttcat cagcaaaggc gtgaacatcg ccattttctg ccgcatggaa ccagttcact
    13381 tgtgctgtgt ggcctgcttt tgccagtgtg tcgagcattg ccagcattgg cgtttgaccg
    13441 acaccggcag agattaacgt cactggtgtg tcatctgcga cagccataaa aaaatcacct
    13501 gctggagcga ccagtttcac gacatcgcca acattggcgt gattgtgcag ccagttggat
    13561 acctgcccac cctcttcgcg tttcaccgca atacgatagc ctttgccatc cggtttgcga
    13621 gtcaaagagt actgacgaat ttcctgatgc gggaaacctt ccggcttcag ccagacgccg
    13681 agatattgcc ccggacggta ttctgccact gcgccaccgt cgaccggctc cagttcgaag
    13741 ctggtgataa gcgcgctgcg cggtgtttta gccacaatgc ggaaatcgcg agtaccttcc
    13801 cagccaccgg ctttgctggc gttttcgtta tagatttccg cctcgcgatt gataaataca
    13861 ttagccagta cgccataggc tttaccccac gcgtccagca cttcctggcc cgggctgaac
    13921 atttcgtcca gcgtcgccaa caggtgttca ccgacaatgt tgtactgttc cggtttgatc
    13981 tggaagctgg tgtgcttctg cgcgattttt tctaccgctg gcagcagcgc aggcaggttt
    14041 tcaatattac tggcgtaggc ggcaatagcg ttaaacaggg cttcacgttg atcgccattg
    14101 cgctggttac tcatgttaaa aatttctttg agttctgggt tatgagtaaa catacggtcg
    14161 tagaaatggg cggttaactt tggtcccgtt tccaccagta aagggatggt ggcttttact
    14221 gtagcgatgg tttgagcgtc aagcatatgg tcttcctttt ttttgcatct taattgatgt
    14281 atctcaaatg catcttataa aaaatagccc tgcaatgtaa atggttcttt ggtgtttttc
    14341 agaaagaatg tgatgaagtg aaaaatttgc atcacaaacc tgaaaagaaa tccgtttccg
    14401 gttgcaagct ctttattctc caaagccttg cgtagcctga aggtaatcgt ttgcgtaaat
    14461 tcctttgtca agacctgtta tcacacaatg attcggttat actgttcgcc gttgtccaac
    14521 aggaccgcct ataaaggcca aaaattttat tgttagctga gtcaggagat gcggatgtta
    14581 aagcgtgaaa tgaacattgc cgattatgat gccgaactgt ggcaggctat ggagcaggaa
    14641 aaagtacgtc aggaagagca catcgaactg atcgcctccg aaaactacac cagcccgcgc
    14701 gtaatgcagg cgcagggttc tcagctgacc aacaaatatg ctgaaggtta tccgggcaaa
    14761 cgctactacg gcggttgcga gtatgttgat atcgttgaac aactggcgat cgatcgtgcg
    14821 aaagaactgt tcggtgctga ctacgctaac gtccagccgc actccggctc ccaggctaac
    14881 tttgcggtgt acaccgcgct gctggaacca ggtgataccg ttctgggtat gaacctggcg
    14941 catggcggtc acctgactca cggttctccg gttaacttct ccggtaaact gtacaacatc
    15001 gttccttacg gtatcgatgc tagcggtcat atcgactacg ccgatctgga aaaacaagcc
    15061 aaagaacaca aaccgaaaat gattatcggt ggcttctctg catattccgg cgtggtggac
    15121 tgggcgaaaa tgcgtgaaat cgctgacagc atcggtgctt acctgttcgt tgatatggcg
    15181 cacgttgcgg gcctggttgc agctggcgtc tacccgaacc cggttcctca tgctcacgtt
    15241 gttactacca ccactcacaa aaccctggcg ggtccgcgcg gcggcctgat cctggcgaaa
    15301 ggtggtagcg aagagctgta caaaaaactg aactctgcgg ttttccctgg tggtcagggt
    15361 ggtccgttga tgcacgtaat cgccggtaaa gcggttgctc tgaaagaagc gatggagcct
    15421 gagttcaaaa cttaccagca gcaggtcgct aaaaacgcta aagcgatggt agaagtgttc
    15481 ctggaacgcg gctacaaagt ggtttccggc ggcactgata accatctgtt cctggttgat
    15541 ctggttgata aaaacctgac cggtaaagaa gctgacgccg ctctgggccg tgctaacatc
    15601 accgtcaaca aaaacagcgt accgaacgat ccgaagagcc cgtttgtgac ctccggtatt
    15661 cgcgtgggta ctccggcgat tacgcgtcgc ggcttcaaag aagcagaagc gaaagaactg
    15721 gctggctgga tgtgtgacgt gctggacagc atcaatgatg aagccgttat cgagcgcatc
    15781 aaaggtaaag ttctcgacat ctgtgcacgt tacccggttt acgcataagc gaaacggtta
    15841 atctgttgaa aaaggcttcc ttcgggaagc ctttctacgt tatcgcgcca tcaaatctgt
    15901 cgtaactgcg cctcaacata caaatagcca atccccagca cctgttgtgc gcggcttaat
    15961 tgcccaaagc caattttcgt cgctttcacg ctcggtcctt cgtcgcggtc gaacgggtta
    16021 aatcccgtct ggcgaataat ggtattaagc cagtgctcac caaaggcaca actgcgaccg
    16081 tacaaccaaa tctgattaat attgaggatg ttgaggaagt tatacagact caaaccaatg
    16141 gcattggcag agcgatcaac ccagctggtg atccacggtt ctccactctg ccaggcagcg
    16201 attaactgcg ctgttgtcag tttttcagga tcaagctgag tattaaccgg ttgtgatttt
    16261 agccataccc gcgcctgttt ttttaatgca cttaacgagg cgacggtttc caggcagccg
    16321 taacgtccac agtcgcagac gacgccatcc ggattaacga tggtgtgacc aatctgtccg
    16381 ctaccataca aacagccgcg ataaatttgc tcgttaatca cgaacgacga gccaatgccg
    16441 taatcaacgt tgatcacgca gaaatcccgt tcctgcgaat tattttgcca tttctccgcc
    16501 agcgccagca tcacgcagtc attatcgacc atcacccgaa tgccgagctt ctcttccagc
    16561 agatacttta cctcaaccgg cgttgtccac ggcgcttgcg gcatggtttg cgacacgccg
    16621 gtcaccggat caacctgacc gtggattgcc agcgccaggt tgatggtacg gtccggccac
    16681 aatttacggt ggcgatgcca gcatttttcg atttcggaca gcagcgcctg cggcgtcggt
    16741 gcatcaatct gtaaatactc aaattcacct ttcggactta aacaagcatt agcaacctga
    16801 cactcaatac tggtgggcgt cacgttcagg cacaacgtcc agtcaccttc cggcgcaatc
    16861 agccatgtac cgctactatg cccgcgcgtc tggctttcat cgtcgatatt caccacccgt
    16921 ttttcgcttt ccagttcctg caaaatatta ctgaccgccg gaatcgaaat ttgcgccagc
    16981 cgggccagcg ttgatttatt ggcgcgcttt tgccggtaca gcagttccag aatcacgcat
    17041 ttgttatggt ggcgaatctg ttgattatta atgcaggctc tcatcttcac taaaccttgt
    17101 ttagttaatt gcgtgattat tccgcgaaaa cattgccaga ttgttcaata cactgccaca
    17161 aatcttttaa ttcagtatgt cttgttaata ttgagggcac catgactcca gtaaaagtgt
    17221 ggcaagagcg cgttgagatc ccgacctatg aaaccgggcc gcaggatata catcccatgt
    17281 tcctggaaaa tcgcgtttat cagggatcgt ccggcgcggt ttatccctac ggcgtgaccg
    17341 atacgctgag cgagcagaaa accctgaaat cctggcaggc ggtgtggctg gaaaacgact
    17401 acatcaaagt gatgatcctg ccggagctgg gcggtcgggt gcatcgcgcg tgggataaag
    17461 tgaaacagcg cgattttgtc tatcataatg aagtcattaa accggcgctg gtggggttgc
    17521 tggggccgtg gatctccggc gggattgagt ttaactggcc gcaacaccat cgcccgacca
    17581 cctttatgcc cgtcgatttc accctcgaag cccatgaaga cggcgcacaa accgtgtggg
    17641 tcggcgaaac ggagccgatg cacggtttac aggtgatgac aggtttcacc ctgcgccctg
    17701 accgggcggc actggaaatt gccagccgcg tctataacgg caacgccacg ccgcgtcatt
    17761 tcttgtggtg ggccaacccg gcagtgaaag ggggtgaagg gcatcagagc gtcttcccgc
    17821 cggatgtaac ggcggtgttt gatcacggca aacgggccgt ctccgctttc cccatcgcca
    17881 ccggcactta ctacaaagtg gactactccg ccggagtgga catttctcgc tataaaaatg
    17941 tgcccgttcc aacctcatat atggctgaaa aatcacagta cgattttgtt ggcgcgtggt
    18001 gtcacgatga agatggcggt ttgctgcacg ttgccaacca ccatattgcg ccaggtaaaa
    18061 aacagtggag ctggggacac agtgaatttg gccaggcgtg ggataagagt ctgaccgaca
    18121 ataacggccc gtatatcgaa ctgatgactg gtatttttgc cgataaccag cccgatttta
    18181 cctggcttga tgcttacgaa gagaagcgtt ttgagcagta tttcctgcct taccattctt
    18241 tgggcatggt gcaaaacgcc tcccgcgatg cggtgattaa actccagcgt agcgagcggg
    18301 ggattgagtg ggggctgtat gccatctctc cgttgaaagg ataccgtctg gcgatccgcg
    18361 aaatcggcaa atgcaacgcg ttgctcgatg atgccgtggc cctgacacca gcgaccgcca
    18421 tccagggcgt gttgcacggt atcaatcctg aaaggctgac cattgaactc tccgatgccg
    18481 acggcaatat cacactgagt tatcaggaac atcagccgca agagttgccg ttgccggacg
    18541 tcgccaaagc gccactggca gcacaagaca ttaccagtac agatgaagcc tggtttatcg
    18601 gtcagcatct ggagcaatat catcacgcca gccgttcacc gttcgattac tacctgcgcg
    18661 gcgtggcgct ggatccgctg gattatcgct gtaacctggc gctggcgatg ctggaatata
    18721 accgagcaga tttcccgcaa gcggtggcgt atgccactca ggcgctgaaa cgcgcacatg
    18781 cgctaaacaa aaatccgcag tgcggacagg cgagtttgat tcgcgccagt gcttacgaac
    18841 gtcaggggca atatcaacaa gccgaagagg atttctggcg ggcggtctgg agcggcaaca
    18901 gtaaagcagg tggctattat ggtctggcac ggctggcggc gcgtaatggt cacttcgacg
    18961 ctggtctgga tttttgccaa caaagtcttc gcgcctgccc aaccaatcag gaagtgcttt
    19021 gcctgcataa tctgctgctg gtgttaagtg gtcgtcagga caacgcgcgt ttgcagcgcg
    19081 agaaactgct gcgtgattat ccgctgaacg ccactctgtg gtggctgaac tggttcgatg
    19141 gtcgtagcga atcagccctc gcgcagtggc gcggtctgtg tcagggacgc gacgttaacg
    19201 ccctgatgac cgccgggcaa ctgattaact ggggaatgcc caccctggcg gcagagatgc
    19261 tgaacgcact ggactgccag cgcacgctgc cgctttactt gcaagccagc ttgctgccga
    19321 aagctgaacg cggcgaactg gtcacaaaag ccattgatgt cttcccacag tttgtccgtt
    19381 tcccgaatac gctggaagaa gtggcggcgc tggagagtat tgaagagtgc tggtttgccc
    19441 gtcatttact ggcctgcttc tactacaaca agtgcagcta caacaaagcc attactttat
    19501 ggcaacgttg cgtagagatg tcgccggagt ttgccgacgg ctggcgcggg ttagcgatcc
    19561 atgcgtggaa taagcaacac gattatgagc tggccgcgcg ttatcttgat aatgcttatc
    19621 agcttgcgcc gcaggatgca cgtctgcttt tcgaacggga tttgctggat aagttaagcg
    19681 gtgtcacacc ggagaaacga ctggcgcgtc tggaaaataa tctggaaatt gcgctgaaac
    19741 gcgacgacat gaccgcagaa ctgctcaatt tgtggcatct cacgggtcag gcagacaaag
    19801 cggcggacat tctcgccacg cgcaaattcc acccgtggga aggcggggaa gggaagatca
    19861 ccagtcagtt tatcctcaac cagttattac gcgcctggca gcatcttgat gccagagagc
    19921 cgcagcaggc cagcgaactg cttcatgccg cgttgcatta tccggagaat ttaagcgaag
    19981 gccgtttacc ggggcaaact gacaacgaca tctggttctg gcaggcgata tgcgccaacg
    20041 cgcagggcga tgaaactgaa gcgacgcgtt gtttacgtct ggcggcgact ggcgatcgca
    20101 ccattaacat tcacagttat tacaacgatc agccggttga ttatctcttc tggcaaggaa
    20161 tggcgctgcg actgctgggc gaacaacaca ccgcacagca actgtttagt gaaatgaaac
    20221 agtgggcgca agagatggcg aaaaccagta tcgaggcgga tttctttgct gtttcacaac
    20281 ctgacctgtt gtcgctgtat ggcgatttac aacagcagca taaagaaaaa tgcctgatgg
    20341 tggcgatgct ggcgtccgcg ggactcgggg aggttgcgca ctatgaatct gctcgcgctg
    20401 aattgatggc gattaatcca gcctggccga aagcggcatt attcaccacc gtgatgcctt
    20461 ttatttttag ctacgttcac taaaccttat taactgtaat atttgagcgg cacgcgctgc
    20521 cgctcacgct caaataatta aaataagccg ttgtaaataa aaaccctaca ggaggcacta
    20581 tgcctaaaaa aatgacaaga acccgtaatt tattattaat ggctacgcta ttgggtagcg
    20641 cactatttgc cagggctgcg gataaagaaa tgactattgg cgcaatatac cttgataccc
    20701 agggatatta cgccggagtg cgtcagggcg ttcaggatgc ggcaaaagat tcttcagtac
    20761 aggtacagtt aattgaaact aacgctcagg gcgatatttc gaaagaaagt acctttgttg
    20821 ataccctcgt ggcgcgtaat gtcgatgcca ttattttatc ggcagtgtct gaaaatggca
    20881 gtagtcgtac cgttcgtcgc gccagtgaag cgggtattcc ggtgatttgc tacaacacct
    20941 gtattaatca aaaaggtgtc gataaatatg tctcggcgta tctggtcggc gatccgctgg
    21001 aatttggtaa aaaactgggt aacgctgccg ccgattattt tattgccaat aaaattgacc
    21061 agccgaaaat tgccgtcatc aattgcgaag cctttgaagt ttgtgtgcag cgacgtaaag
    21121 gatttgaaga ggtattaaaa tcccgcgttc ccggcgcgca aatagtcgct aatcaggaag
    21181 ggactgtttt agataaagcg atttccgttg gtgaaaaact aattatctcc acgccggatc
    21241 tcaacgccat tatgggggaa tcgggcggtg cgacactcgg cgcggtaaaa gcggtacgta
    21301 atcaaaatca ggccggaaaa attgctgttt tcggttcgga tatgaccact gaaattgctc
    21361 aggagctgga aaacaatcag gtgctgaaag cggtggtgga tatttccggt aagaaaatgg
    21421 gcaatgctgt tttcgcgcaa actttgaagg ttatcaataa acaagccgac ggtgaaaaag
    21481 tgattcaggt gccaatcgat ctctatacca aaacggaaga cggtaaacag tggctggcaa
    21541 cgcacgttga tggtctgccc taatcgctga ttcgcagagg tccctatgtt cacagcaaca
    21601 gaggcagtcc cggtagcaaa agtggtggca ggaaataagc gttatcccgg cgtcgttgcg
    21661 ctggataacg ttaacttcac gctcaataaa ggcgaagttc gtgcgctgtt aggcaaaaac
    21721 ggtgcgggaa aatcgaccct cattcgaatg cttaccggta gcgaacgtcc ggatagcggt
    21781 gatatctgga ttggcgagac gcgactggaa ggtgacgaag ctacgctgac tcgccgtgcc
    21841 gctgaactgg gggtacgcgc ggtttatcag gagttaagtc tggtggaagg gctgacagtg
    21901 gcggaaaacc tctgcctcgg tcagtggccc cgccgcaacg gcatgattga ttacctgcaa
    21961 atggcgcagg acgcccaacg ttgcttgcag gcgttgggcg ttgacgttag tcctgaacaa
    22021 cttgtttcaa cactaagccc ggcgcaaaag cagctggtgg aaattgcgcg ggtgatgaag
    22081 ggcgagccgc gcgtggtcat tcttgatgaa cccaccagct cgcttgccag tgcggaggtt
    22141 gaactggtga tcagcgcggt gaaaaagatg tcggcactgg gcgtggcggt gatttatgtc
    22201 agccaccgga tggaagaaat tcgccgcatt gcctcctgtg ccaccgttat gcgcgatggt
    22261 caggtggcgg gcgatgtgat gctcgaaaac acctccacgc atcatattgt gtcgctaatg
    22321 ctcgggcgcg atcacgttga tattgcgccg gtttcccctc aggaaattgt cgatcaggcc
    22381 gtgctggaag tccgtgcgtt acgccataag cccaagctgg aggatatcag ctttacgctc
    22441 cgtcgtggcg aagtactcgg cattgctggc ctgctggggg cagggcgcag tgaattgttg
    22501 aaagccatag ttgggctgga gacgtatgaa cagggcgaaa ttgttatcaa cggcgagaaa
    22561 atcactcgcc ccgattacgg cgacatgctg aaacgcggca ttggctatac gccagaaaac
    22621 cgcaaagatg cggggatcat tccctggctg ggcgttgacg aaaatacagt gctgaccaat
    22681 cggcaaaaaa tcagcgccaa cggtgtgctg caatggtcca ccatccgccg cctgaccgaa
    22741 gaggtgatgc agcggatgac ggtcaaggcc gccagtagcg aaacgcccat cggcacgctt
    22801 tctggcggca atcagcaaaa agtggtgatc ggtcgttggg tctatgccgc cagccagatt
    22861 ttgttgctcg acgagccaac gcgtggcgtc gatatcgaag ccaaacagca gatttaccgt
    22921 attgtccgcg agctggctgc cgaaggaaaa agcgtggtgt ttatctccag tgaagtggag
    22981 gagttgccgc tggtgtgcga ccgcattctg ttgttacagc acggtacgtt ctcgcaggag
    23041 tttcacgctc cggtcaatgt ggatgagctg atgtccgcca ttctgtctgt gcactgatga
    23101 attttaccga ggaacctgaa gatgtctgct tcgtcattac tattgccgca gggcaagagc
    23161 gtctcgctca aacaatttgt cagtcgccat attaatgaga tcggtttgct ggtggtgatc
    23221 gccattcttt atctggtctt ctctctgaac gcgcctggct ttatctcatt gaataaccag
    23281 atgaacgtgc tgcgcgatgc cgccaccatc gggatcgccg catgggcgat gacgctgatt
    23341 attatctccg gtgaaattga tgtcagcgtt ggtccgatgg tggcttttgt ctcggtgtgc
    23401 ctggcatttt tgctgcaatt tgaagttccg ctggcggtgg cgtgtctgct ggtgttgctg
    23461 ttaggcgcgc tgatgggaac gctcgccggg gtgctgcgcg gcgtgtttaa cgtgccaagt
    23521 tttgttgcca cgctgggttt atggagcgcc ctgcgcggaa tggggctgtt tatgaccaac
    23581 gccttgccag tgccgattga tgaaaacgaa gtgctggact ggctgggggg acaatttctc
    23641 ggtgtgccgg tatccgcgct gatcatgatg gtattgtttg cgctgtttgt gttcatcagc
    23701 cgcaaaaccg ccttcgggcg ctcggttttt gccgttggcg gtaatgccac tgcagcacag
    23761 ttgtgcggta ttaacgttcg tcgggtacgc attcttatct ttaccctttc gggattatta
    23821 gcggcggtga ccggcatttt gttggcggcg cgcctcggtt ccggtaacgc aggtgccgca
    23881 aacggtctgg agtttgacgt catcgccgcg gtcgtggttg gcggtacggc gctttccggt
    23941 ggtcgcggct cgttgttcgg tacgctgctt ggcgtactgg tgattacgct aatcggtaac
    24001 ggtctggtgc tgctcgggat taactccttt ttccagcagg tggtgcgcgg cgtcatcatc
    24061 gtggtggcgg tgctggcgaa tatcttgctg actcagcgta gcagtaaagc gaaacgctaa
    24121 accttaacgc ccctggcgcg ggtgcggcag ggatgaaaat taatcgtatt gttaatgagg
    24181 gatttatgaa aacgatgctg gcagcttatt taccaggaaa ttcgaccgtc gatctgcggg
    24241 aagttgcggt gccgacgccg gggattaacc aggtactgat caaaatgaaa tcctccggga
    24301 tttgcggaag cgatgtccac tatatctatc atcaacaccg tgccacggca gcggcacccg
    24361 ataaaccgtt ataccaggga tttatcaacg gtcatgaacc gtgcgggcag attgtggcga
    24421 tggggcaagg ctgccgccat tttaaagagg gcgaccgtgt gctggtgtat cacatttctg
    24481 gctgtggttt ttgcccgaac tgccgtcgcg gctttcctat ctcttgtact ggcgaaggaa
    24541 aagcggctta cggctggcag cgtgacggcg gtcatgccga atatctgctg gcggaagaaa
    24601 aagatctgat cctcctgccg gatgcactga gttacgaaga tggtgcgttt atcagttgcg
    24661 gcgttggtac agcgtatgaa gggattttgc gcggcgaagt ttccggcagt gataacgtac
    24721 tggtggtcgg tctggggccg gtcggcatga tggcgatgat gctggcgaaa ggtcgcggtg
    24781 caaaaaggat catcggcgtt gatatgctgc cggaacgtct ggcgatggcg aaacagttag
    24841 gagtgatgga tcacggctat ttagcgacca ccgaaggtct gccgcagata atcgccgaac
    24901 tcactcacgg cggcgcggat gttgcgcttg attgttccgg taatgccgca ggtcgcttac
    24961 tggcactgca atccaccgct gactggggac gcgtggttta cattggtgaa accggaaaag
    25021 tcgagttcga ggttagcgcc gacctgatgc accatcaacg gcggattatc ggctcctggg
    25081 tgaccagtct gttccatatg gaaaaatgcg cccacgattt aacggactgg aaactgtggc
    25141 cgcgtaacgc cattacccac cgcttctcgc tggaacaggc gggagatgct tatgcactga
    25201 tggcaagcgg caaatgcggg aaagttgtga ttaacttccc ggattaagga gttagcggat
    25261 gaccatctat accttatccc acgggtcgct aaagctgggc gtttctgacc agggcggcgt
    25321 aatcgaaggc ttctggcgcg atacgacgcc gctcttacgt cctggtaaaa aaaacggtgt
    25381 ggcaaccgat gcctcttgtt ttccgctggt gccatttgca aaccgggtga gcggcaaccg
    25441 ttttgtctgg caggggcgtg agtatcaact gcaaccgaat gttgagtggg atgcgcacta
    25501 tctgcacggc gacggctggc tggggcagtg gcaatgtgtc tcgcgcaatg atgatatctt
    25561 gtgtctggtg tatgaacatc gcagcggtgt ttatcactat cgggtaagcc agacgtttca
    25621 tttaacggcg gatacgctga cggtgacact ctctgtcacc aatcaagggg cagagacgct
    25681 gccatttggt accggctggc atccttattt tccgttgtcg ccgcaaacgc ggattcaggc
    25741 acaggcgagc ggttactggc tggagcggga gcagtggctg gcgggtgagt tttgcgagca
    25801 gctaccgcaa gaattggatt ttaaccagct cgcgccgttg ccgcgccagt gggtgaacaa
    25861 tggttttgcc ggatggaatg gtcaggcccg catcgagcag ccgcaggagg gttatgccat
    25921 catcatagaa acgacgccac ctgcaccgtg ttatttcatc tttgtttccg acccggcgtt
    25981 tgataaagga tatgcgtttg atttcttctg tctggaaccg atgagccatg cgccagatga
    26041 ccatcatcgc ccggaaggtg gtgacctcat tgcgcttgca ccaggggaat caacaacttc
    26101 agagatgtcg ttgcgggttg cgttactgta atttataaaa tgccggataa ggcgttcaca
    26161 ccgcatccgg cactcggcgc acgatgcctg atgcgacgct tgccgcgtct tatcaggcct
    26221 acaattggtg ttggaacggt aggcaggata aggcgttcac gccgcatccg gcatctttcc
    26281 taacgccgat cgagagaaat cgcccccggc ccggtaattg ccaacagcag gaatgcgcca
    26341 gcgatactga cattcttcca gaaattaatc atatttggcc caaccgcatc gccggtcata
    26401 tcccagtaat ggtgaccaat caccgccgta cccagcgtgt agaaaataaa cagcaccgcc
    26461 agcggacggg tgaaaaagcc aagcacgatt aatatcgcgg cgggcacttc cataactacc
    26521 gcaataatcg ctgccagcat cggcatcggc gcgcccagcg aggccatata ttggaccgta
    26581 ccgtcaaagc ccatcatttt gggaaaacca aaaataatga aaattaagac cacggcgata
    26641 cgggcaatta ataacaaaac ggggcgtgca gcaccaaaat caaaataacg tagtgagttc
    26701 ataaccaatc cctttgcaag ataatgtgat ttaaacgtaa tacacactct tgcacctcgc
    26761 cacgcccctg gcattgagat attcgctaat tatcctggtt acagtgattt aagcgcgatg
    26821 tcctcatcta tcagcagttt cgcatcaaac gttttgccgc tctggatcca tttgcggatt
    26881 aagcgaatct cacgcccctg attcagcgtt accgcaccga taagcacgcc gttttgcaga
    26941 ttaaaccaaa tcgccttctg agtttccggg ttgccacgac aaatccagtc atcgccatgc
    27001 atatcgccaa taaactgtaa gttatcactg tactgatcgc tccagaacca cggcggcggc
    27061 agtcgcggaa gcggtagccc caacattgcg gcagcggcaa tttgcgcctg gttattggcg
    27121 ttttcccagc tttcgcagcg gtgtagtgca ccattatcaa tacgggtgat tgccacatcg
    27181 ccaccggcaa agatcgcggg atcgcaggtg cggcaagctt catcaatgac aataccattg
    27241 gcagtatcaa ggttggcctc gcgagccagt tggtcgttgg cgctgatacc aataccgtaa
    27301 atcaccacat cggcccgaag cgtttcgcca ctttgcagcg tcagttctac tttttcacca
    27361 tcgaccacat gttcaatggc attattgagc agaatgcgca caccagcctg ctgatggcgc
    27421 ggtaaaagat agcgttgcac gggcggtggt gcattacggc ccatgacgct tgccgccagt
    27481 tcaatcactg tcaccttaca tccacgctgc gtggcgctgg cagccagttc cagaccaata
    27541 gttccggcac cgacaatcac gactgaccgt tcgggctgca gaacttctcg cagtctggcg
    27601 gcatcaccgg catggcgcag ggtaaagcag cgttctccca gtgcatcaag caacggcagc
    27661 ggtcgagctg ccgcgccggt tgctataaaa agctgatccc agtgccagct ttcgccgtta
    27721 gctaacacta actcttgtgt gtcgcggccc aatgttttga tggttacacc ggaatgcaga
    27781 tgaacattgt tttcctgcca ccagtgagcg ggtaacacag actgcaactg tggggaatct
    27841 tccagcaaca tggatttcga gagcgggggg cgttcataag gaagatgttg ctcatcggaa
    27901 aacagatgca gctcaccgct gaacccttgc tggcgtagca aggccgcagc cattgccgcc
    27961 gcttgcccgc caccgacaat aatgatcgtt ttttctttca tcgtgcttcc ttaaagatcc
    28021 agcccagccg ctacgtggcg aatgccgcga atcgctaaac ccgcatcagc gttgatcatc
    28081 acaccgctta atgcgcgatt attgcgccgc gatgccaaca tcacatacgg acccgtaaaa
    28141 tccgccggtt gcgggaaaaa ttgcagcggt aaaatggcgg caattttctc cggcgtcaga
    28201 gactgcatta tcgaggtttc actttgcccg agcgcctgtg ggccgcgcag gtcgctggcc
    28261 ataccacacg ggccgacgcc attcacccgc actttcggtg ccagttcata agccagttgg
    28321 cgaataagtc cggttgcggc atgtttactg gcggtgtaca gcgggccacc gccgccggga
    28381 taccaggcgg cgtttgacag tgtgaaaatc atgctgcctt cactggcgat taacgccgga
    28441 gcgcaggctt ttgcgcccag caggtaaccg agtacgttga cattaaacag ctcgtggaag
    28501 ccggtttcga gcgtctctgc gggagtgtta accagtgagg cattgtgatc ccagatgccc
    28561 gcattgccga taaaacaatc cagcttgcca gaacgagtca ggatctgatt aaccgcgcgt
    28621 tgataatcgg catagcaggt cacgttacct tccaccgcca gaatatgttc gccaaatcgc
    28681 tgacgcagac tggcgacttt tgccgccgac agttccagcg tggcaacctg cgcgccttct
    28741 tcgataaatc gctcggccag cgccaggcct aatcccgatc cgccgccggt aataaagatg
    28801 gactcgttat gcagatcgct catggctgcg cctccggtaa gtcgatgaag atgtcaccac
    28861 cttcaacgtg tactggataa gtggttagcg gatcggtggc gggcaggcat aacgctttcc
    28921 ccgttttcag gcaaaaactg gcggcgtgca gcgggcactc caccgtggcg tcatcttcca
    28981 gatacccttc tgacatcgac gcattaccat ggctgcaacg atcgttaatg gcataaaact
    29041 cgccgcctac gttgaacagg gcgatgacgg gcgaggtatc gatccggaga gcctcaccct
    29101 ccggcacatc cgcaacggga cacgcataaa ttcgattcat cagaacagta cactcaggtt
    29161 atgggaggag atgaccgctt gatccaggac gatatcccgt tccagcaagc gccagttccc
    29221 gtcctccaga cggcgaactt tgtcgaaacg cgttccaaca tagaatgttt catcgcgctc
    29281 tttttgtgcc cgataaagta gataattcac cctcacggca aatacgtttg ggatgtcggt
    29341 ttcgcttacc tggcagttgc tgattaagtg acgagtgcgt gacggcggtt cttctgccca
    29401 ggccatgccg gtttccagac gggcgattcg ccgttccagc tggtctttgg tgtcattaaa
    29461 aatccaggtt gtcggcggtt gaacgccttt gcggcggtcg cgcgtttgtg cgttaaccgt
    29521 ggtgcgcatg gtgtaacgaa tctcttcgtc gagctgtgcc agccagtcac gaaatttcca
    29581 gtcatccagc agactggctt cgtgaaagag aaactggcta atgcggtgat gtaactctag
    29641 tgaaacttgc gcactcattt catcacctcc tgctggtaag cggcggtttt atcgagcact
    29701 tcctgccagc tttcgctact cagaagatcg gcccagcgtt ggtacattcc acgagcggcc
    29761 gtttctgaga agatatagtt agtaatgcct ggaatgccgt cgtcgcgacg cttttcctga
    29821 ccaagcccca tttccagaca cagtttgctg ttgcgggcgc ggtggccttt cagcaatttc
    29881 tggatttcac accagttctc cgagtcatcc tgctcgagaa aaccagcagg accaaaagca
    29941 cgagtggcgc tgttttcaaa agcggcttta acttcatcgg aggcggcttt gtcagtaata
    30001 cagaacgccc acacttcgac ttgatcaggg ccgcgcggat gccagacgcg gagcgtggca
    30061 gtgccgttga gccatgaaag cgtggggaaa atattgttat gacccgccag gcgcagggcg
    30121 cgaacttcac ctaaacgttg ttctgcttcg gcataggttt cgcggtaata gctggaaacc
    30181 gcgccatcga cccagacatt agcatccggt ttttcagtaa agaagaaacc gctaccgtga
    30241 ccgtcctgac caaattgcag cgcatctttg gcggtttccc acaccgggcg ggcggtttgt
    30301 ccatcaccga gacgcttatc gctgccatca tctttcgccc ctaatacctg aacggcagaa
    30361 gcatggctga acagagcatg atattggtca ctggcgaact gctctgccgg gaatttccag
    30421 ttgcagttga tcacccactt ttgtacaccg ccgacaattt cggtgccgcc ttcgcgacga
    30481 tccagcatgc catccagata ccaggcaatg tcacccaggt aatcacgcag gcccggtgcg
    30541 ctggtatccc agttgccaaa aatcagccct ttataactct ccacacaagg aacttcgttt
    30601 agtccccagt gggatttaca caacccttgt gggtaggcgc gaggttccag cggtacatcg
    30661 atcaactcgc cgttaatgcc ataagaccag ccgtgatacg gacaggtaaa ggcgcgagtg
    30721 ttaccgcaat ctgcataact cacacgcatg gcccggtggc ggcattggtt gagaaacgcc
    30781 ttaatgctgc cgtctttctg acgcactacg acaaccgcat cttcccccat gtaggtgtta
    30841 aagaaatcac catgttttgg gatctggctt tcgtgggcga gaaatagcca gcaacgaccg
    30901 aaaatgcgct caagctccag ttggtaaata tccgggtcgg tataaatacg cggagtgacc
    30961 cgaccgtttt gggtgtcaat cagttggtaa atgttcaaat ctgagggtgt ggtcatattt
    31021 tttaatcctg aaagataaga tgtattgacc ttctattttt gtgccataag gatgttgctg
    31081 gttggttgct gatatgtgaa atagttaatt tctaccagct gagacgtttt ttcgataaca
    31141 aggggaaggt gatggaacta cggcatttac gctatttcgt cgcagtggcg caggcactga
    31201 actttacccg tgcggcggaa aaactgcata cctcacagcc ttcgttaagc agccagatcc
    31261 gcgatcttga aaactgtgtc ggtgttccgt tgctggtgag ggataagcgc aaagtcgcgt
    31321 tgacggcggc gggagaatgt tttctccagg acgcgctggc aatcctcgaa caagcggaaa
    31381 atgccaaatt acgggcgcgg aaaattgttc aggaagacag acaattaacc attggctttg
    31441 tgccatcggc ggaagtgaat ttactgccaa aagtattacc gatgtttcgt ctcagacagc
    31501 cagacacctt aattgagctg gtgagtttaa tcaccacgga acaggaagaa aaaattcgcc
    31561 gtggtgaact cgacgtcggc ttaatgcgcc atcctgttta tagcccggag attgattatc
    31621 tggagctttt tgacgaacca ttagtggtgg tgttaccggt tgatcaccct ttagcacatg
    31681 aaaaagagat cactgccgcg caactggatg gcgtgaattt cgtcagtact gatccagcgt
    31741 attccggttc gcttgcgccg atcgttaaag cgtggtttgc gcaagaaaac agccagccaa
    31801 atatcgtcca ggtggcaacg aatattctgg tgaccatgaa tctggtgggg atggggctgg
    31861 gcgtcacttt gatacccggt tatatgaata attttaatac cggccaggtg gtatttcgac
    31921 ccattgccgg aaatgtgcct gccattgctt tactgatggc gtggaagaaa ggggagatga
    31981 agccggcatt acgcgatttc atcgccattg tgcaggaacg tttggcaagc gtaacggcat
    32041 aattacgcat tatttttgca aaacgcgtcg cggatcgcag ttttttagtg cttgtgaact
    32101 ggcgttacag acatcgggaa gcaggctcga atattcttat ttttcaactt gcaccaacat
    32161 tcgattaacc ccagactaac gcctcctgac gggagggact catggttttg caatccacgc
    32221 gctggttggc gctcggctat ttcacatact tttttagtta cggcattttt ctacctttct
    32281 ggagcgtttg gcttaagggg attggtttaa cgccagaaac catcggcctg ttattggggg
    32341 caggtctggt tgcccgtttt ctcgggagct tgctcatcgc gccccgcgtc agcgatcctt
    32401 ctcgcctgat ttccgccttg cgcgtgctgg cactgctgac acttctcttt gctgtcgcct
    32461 tctgggcggg ggcgcacgta gcgtggctga tgctggtgat gattggcttt aacctctttt
    32521 tctcaccgct ggtaccgttg accgatgcac tggcgaatac gtggcaaaag cagttcccgc
    32581 ttgattacgg caaagtgcga ttgtggggct cggtggcgtt tgtcattggc tcgtcgctga
    32641 cgggcaaact ggtcactatg tttgattatc gggtgatcct cgcgctgttg acgttgggcg
    32701 tggcatccat gctgctcggc tttctcatcc gtccgacgat tcagccacaa ggggcaagcc
    32761 gccagcagga gagcaccggt tggtctgcgt ggttggcgtt ggttcgccag aactggcgct
    32821 ttctggcctg cgtttgttta ttgcaggggg cgcatgcggc ctattacggt tttagcgcca
    32881 tttactggca ggctgctggc tactcggcct cggcggtggg ctatttgtgg tcgctgggcg
    32941 tggtggcgga agtcattatc tttgcgctga gtaataaact tttccgccgt tgtagtgcac
    33001 gcgatatgct gttgatctca gcgatttgcg gcgtagtgcg ctggggcatt atgggagcaa
    33061 ctacggcgtt gccgtggttg atagtggtgc aaattctgca ttgtggcacc ttcacggtct
    33121 gccacctggc cgccatgcgc tatattgccg ctcgccaggg tagcgaagtc atccgtttac
    33181 aggcggttta ctctgccgtc gcgatgggcg gcagtattgc gatcatgacc gttttcgccg
    33241 gtttcctgta tcaatatctg ggccacggcg tgttctgggt gatggcgctg gtggcgcttc
    33301 cggcaatgtt tttgcgcccg aaagttgttc cctcatgctg attcgagcat tttgcggatc
    33361 tgctcctgtt gatgtgttgt caacgtcagg tccgtataga tcagcggtgg tgaaaagagc
    33421 ggtaacggca tggtataagg agcaataatc agtgccgcgc tgcgcggagc gcctgtctgc
    33481 aaaaatgcct ttaccgacat atgtttaata ttgagcggca gtaacgttag ttcgcgtagc
    33541 tgctgctcaa tctgcgcttc tcgctcgcta tcattcccgg tcagtagaat aatctgtttc
    33601 tcatgcaggt cgttttcctg cattagccag gcaccaaaaa tcaccgcgac cagaccgctt
    33661 tcctcatcag aaagatggac gccgtattca ctttcaaatc cggccagcgc cgcgcgggtg
    33721 gtgcgcacca ggcgtggata caggcgggcg aactcttccg gcagggtgtt atcaataccg
    33781 atggcaaaca aactgcgatt taacgcctgg gcgaggtggg tataaagctg atcgcataac
    33841 ccctgttcat cataaaaacg aacatttccc agctcacgaa aatggtttac cagacgtttg
    33901 atagacttac gcagttgtct gtcccgctga tgcgcatccc gcagtggatc gggaacccgc
    33961 aacatggaaa aaagtaatgc cataaacagt ggctcatcag gcggtacagg ctggagcgcc
    34021 cgacgctgcc agtggcgtcc aatttcctgc gctacctgaa attcaaggca ggattccgcc
    34081 cagcgacgtt ggagcggatt aaactgaggc gtaattccag cgtggtgttg cagcaaacaa
    34141 tattgcagat acagacacag gaaatgaata tcgcgcgttt caaagcgttt ttgcagccgt
    34201 cgggagcaga gattcactaa cgcttgtaga ttggtatcgt cgtaaaagtt acgcgcaatt
    34261 ccgcgccgct tcagttcact cttcagggcg ggggtgaaat ggctggtaat aaatgacgga
    34321 cacagacgga aaccacgtcg tagccagtga aataaacaca aacgttggtt aagcactgta
    34381 ccttcaaccc ggtagctacc gtcataacca gtagtcagtg tgagttgatg atagcgcagg
    34441 atctcccgtc ctgtttcgct gatatcaaga ctggcaatat catcatccac accattaagc
    34501 tcgctgaaag ttgccgtggt ggcggtcaac cccggctgaa agagcgtcag caagatctgg
    34561 cagcggcgct ggggagccga aaggacagat ggtggagcaa gcgtaggcat catctgtaga
    34621 tctcccataa gcataattga taagaatagc taaagtttgc tcagggaaat ggcgaaggga
    34681 atgctaatca tcagaaatgt tggcaagcgt cacagaattt ttctcatctt aaactgttct
    34741 gaccagtgga ttagcatgtc ttaaggtggt tttgtgaatg attatcgtgc tagcgatcat
    34801 gggttagttc tgcggattca acgcgctaag gataaaatcc accatctgtt cgcgataaac
    34861 atcgccgtga cgatcggaaa aagcatcaat gtgttcgcca tccgggatta ggatcaaccg
    34921 ttttggctct tttgccaggc tatacaactt ttcgctgtgc tgccatggga taacgtgatc
    34981 agctttaccg tgaatcagta aaagcgggat cgggctgacg ctggcgatgt aattttcgcc
    35041 gctgtaactc tcatcaagta agtagccact gccggggatc atctggttgg cgatggtcgc
    35101 ataagaggca aatgtggagt cgaggatcac cgcacgtatg ccttcacgat caccctgacc
    35161 aataacatcc aaaatattcg ccccgccaat actctgcccg aacagcacca gccgttgtgg
    35221 gtttacatca ctgcgatggc gcaccacatt gatggcactt tgcgtatcgt ccagcaatcc
    35281 ggcctgggac ggcgtgcctt ttgatttacc aaacccgcga taatcaaaca taaaaacgtt
    35341 gaaattacgc tcgggtaacc aactgaccag cggccagtgg gcggacatat ttccggcatt
    35401 gccgtgagca tgaataatgg tggcaatggc cttttcagcg gggccagttg cagaagggat
    35461 aaaccaacca tgtaggcgag tgccatcttt agcggtgaat tctactgatt cggctgacca
    35521 gggatcgggg ccgtagattt tgtcgtcagg gtaatagaaa aaattgatgg cgatgcgcgg
    35581 tattaaataa atgcagaagg caacaacaaa gagaataaac agaattttga gaacgcgttt
    35641 gttcactggc agcgccataa cctttccttg cgacttcttt atcatccatt aatcggggaa
    35701 agatggtggg agagtggcga aagaaagtaa agcggtgagg aagtatttcg gaatagcgcc
    35761 tgaaaggcga gggcgggtga gtgacatcac ccgccagagt cattaacgct tcagggcgtc
    35821 gcttaactcg tcacgcatgt tcgccagcat ggctttaaca acgcgcgggt taccagcaac
    35881 gatgttaccg gtcagcatgt agttatgacc accggtgaag tcgctgacga tgccgcccgc
    35941 ttcacgaacc agcagctcgc ctgccgcgaa atcccacgga cgcagaccga tttcaaagaa
    36001 accgtcaacg cgacccgcag caacgtaagc cagatccagc gccgcagaac cggtacgacg
    36061 gaagtctgca cattcgttga acagtttgcc gacgatgttg atgtaggtag tggcgtactg
    36121 ttttgctttg aacgggaagc cggtcgccag aatagtaccg tcgagatcgc gagcggtgct
    36181 gccgcgcaga cggtagccgt tcagctgtgc gccctgaccg cgagtggcgg tgaacagttc
    36241 gttacgcata ggatcgtaaa ccacagcaac ttcagtgcgg cctttgatac gaacggcgat
    36301 agataccgcg aagtgcggca gacgtttgat aaagttggta gtgccatcca gtggatcgat
    36361 aacccattga acatcctgat cagtaccttc aagttcaccg ctttcttcgg tgatgatggt
    36421 gtgctgtggg taagatttac gaatcgtgtc gataatcacc gcttcggcag ctttatctac
    36481 gttggtcacg aagtcgttac tgcctttctg gctcgcttct acagcgtccg gggtttcata
    36541 gtttttggca attaaattac ccgccttgcg cgctgcgcgc acggcgatgt tcagcatcgg
    36601 atgcatcggt ctctctcact ggatgttaaa gaacgggaaa acggcgcaga gtatagcagc
    36661 gagatcggca tctgtcttcg ttttatgata agatgcgtga ataatcttta gatgaagaaa
    36721 gacaatgctg caaaatattc gaattgtgct ggtggagacg tcacacaccg gcaatatggg
    36781 gtctgttgcc cgtgccatga aaacaatggg attaaccaat ctgtggctgg ttaatccact
    36841 ggtgaaacct gactcccagg cgattgccct ggcagcaggg gccagcgatg tgattggtaa
    36901 tgctcacatc gtcgatacgc tcgacgaagc gttagccggt tgtagcctgg tggtgggcac
    36961 cagcgcacgc tcccgcacgc tgccgtggcc gatgctcgac ccgcgcgaat gcggtctgaa
    37021 aagcgtcgct gaagcggcaa ataccccggt ggcactggtg tttggtcgcg agcgcgtcgg
    37081 cttgaccaat gaagagctgc agaaatgcca ttatcatgtc gcgattgcgg ctaacccgga
    37141 atacagttcg ctgaacctgg cgatggcggt tcaggttatt gcctatgaag tgcgcatggc
    37201 ctggctggca actcaggaaa atggcgagca ggtcgagcat gaagagacgc cgtatccgct
    37261 ggtcgatgat ctggagcgtt tttatggtca tctggaacaa acgctgctgg caaccggttt
    37321 tattcgtgaa aaccatccgg ggcaggtgat gaataaattg cgccgtctgt ttacccgtgc
    37381 gcgcccggaa agccaggaat tgaatatcct gcgcgggatt ctggcttcta tagagcagca
    37441 gaataaaggt aacaaggccg aataatagtg gttgcctgat gcgacgctta acgcgtctta
    37501 tcaggcctgc agtgcacaga gcccctgggc ggataaggcg ttcacgtcgc atccgacaac
    37561 aggtacaaac gccacgataa aaaaatggca ctgaaggtta aatacccgac taaatcagtc
    37621 aagtaaatag ttgaccaatt tactcgggaa tgtcagactt gaccctgcta tgcaataccc
    37681 ccacttttac aataaaaaac cccgggcagg ggcgagtttg aggtgaagta agacatgaga
    37741 ctgacatcta aagggcgcta tgccgtgacc gcaatgcttg acgttgcgct caactctgaa
    37801 gcgggcccgg taccgttggc tgatatttcc gaacgtcagg gaatttccct ttcttatctg
    37861 gaacaactgt tttcccgtct gcgtaaaaat ggtctggttt ccagcgtacg tggaccaggc
    37921 ggtggttatc tgttaggcaa agatgccagc agcatcgccg ttggcgaagt gattagcgcc
    37981 gttgacgaat ctgtagatgc cacccgttgt cagggtaaag gcggctgcca gggcggcgat
    38041 aaatgcctga cccacgcgct gtggcgtgat ttgagcgacc gtcttaccgg ttttctcaat
    38101 aacattactt taggcgaact ggttaataac caggaagtgc tggatgtgtc tggtcgtcag
    38161 catactcacg acgcgccacg cacccgcaca caagacgcga tcgacgttaa gttacgcgct
    38221 taataaaaag aattcagaat caggccggag tgctaaacac tccgtaaaca cggtcgtaca
    38281 tccagccggt agcctggttc cttgcattga gtgatgtacg gagtttatag agcaatgaaa
    38341 ttaccgattt atctcgacta ctccgcaacc acgccggtgg acccgcgtgt tgccgagaaa
    38401 atgatgcagt ttatgacgat ggacggaacc tttggtaacc cggcctcccg ttctcaccgt
    38461 ttcggctggc aggctgaaga agcggttgat atcgcccgta atcagattgc cgatctggtc
    38521 ggcgctgacc cgcgtgaaat cgtctttacc tctggtgcaa ccgaatctga caacctggcg
    38581 atcaaaggtg cagccaactt ttatcagaaa aaaggcaagc acatcatcac cagtaaaacc
    38641 gaacataaag cggtactgga tacctgccgt cagctggagc gcgaaggttt tgaagtcact
    38701 tacctggcac cgcagcgtaa tggcattatc gacctgaaag aactcgaagc agcgatgcgt
    38761 gacgacacca tccttgtttc catcatgcac gtaaataacg aaatcggcgt ggtgcaggat
    38821 atcgcggcta tcggcgaaat gtgccgtgct cgtggcatta tctatcacgt tgatgcaacc
    38881 cagagcgtgg gcaaactgcc tatcgatctg agccagttga aagttgacct gatgtctttc
    38941 tccggtcaca aaatctatgg cccgaaaggt attggtgcgc tgtatgtgcg tcgtaaaccg
    39001 cgcgtacgta tcgaagcaca aatgcatggc ggcggtcacg agcgcggtat gcgttccggc
    39061 actctgcctg ttcaccagat cgtcggtatg ggcgaagcct atcgcatcgc aaaagaagag
    39121 atggcgaccg agatggaacg tctgcgcggc ctgcgtaacc gtctgtggaa cggcatcaaa
    39181 gatatcgaag aagtttacct gaacggtgac ctggaacacg gtgcgccgaa cattctcaac
    39241 gtcagcttca actacgttga aggtgagtcg ctgattatgg cgctgaaaga cctcgcggtt
    39301 tcttcaggtt ccgcctgtac gtcagcaagc ctcgaaccgt cctacgtgct gcgcgcgctg
    39361 gggctgaacg acgagctggc acatagctct atccgtttct ctttaggtcg ttttactact
    39421 gaagaagaga tcgactacac catcgagtta gttcgtaaat ccatcggtcg tctgcgtgac
    39481 ctttctccgc tgtgggaaat gtacaagcag ggcgtggatc tgaacagcat cgaatgggct
    39541 catcattaat cggtatcgga atcaggagaa tttataatgg cttacagcga aaaagttatc
    39601 gaccattacg agaatccgcg taacgtgggt tcctttgaca acaacgacga gaacgtcggc
    39661 agcggcatgg tgggtgcacc ggcctgtggc gacgtgatga agttgcagat taaagtcaac
    39721 gatgaaggta tcattgaaga cgcgcgtttt aaaacttacg gctgcggttc tgctatcgct
    39781 tccagctccc tggtcaccga atgggtgaaa gggaagtctc tcgacgaagc gcaggcgatc
    39841 aaaaacaccg atattgctga agaacttgaa ctgccgccgg tgaaaattca ctgttctatt
    39901 ctggcagaag acgcgatcaa agccgccatt gcggactata aaagcaaacg tgaagcaaaa
    39961 taagagttga ggtttggtta tgtcgattac actgagcgac agtgcagcag cgcgagtaaa
    40021 tacctttctg gctaaccgcg gcaaagggtt tggcctgcgt ctgggcgtga gaacctccgg
    40081 atgttcaggt atggcttatg tactggaatt tgttgacgaa ccgacgccgg aagacatcgt
    40141 gtttgaagac aaaggcgtga aagtcgtggt cgatggcaaa agcctgcaat ttctggacgg
    40201 tacgcagctg gacttcgtaa aagaaggcct gaacgaaggg tttaaattca ccaacccgaa
    40261 cgtcaaagat gagtgtggtt gcggcgaaag cttccacgtt tgatgcgcat accgataacc
    40321 ccaccgtggt cgcctgcgcg tggggtttgt tttacctgat tcgccgttat cgcggcggat
    40381 cgcagccctg agaatgttat ggattacttc accctctttg gcttgcctgc ctgttatcaa
    40441 ctcgataccc aggcgctgag cctgcgtttt caggatctac aacgtcagta tcatcctgat
    40501 aaattcgcca gcggaagcca ggcggaacaa ctcgccgccg tacagcaatc tgcaaccatt
    40561 aaccaggcct ggcaaacgct gcgtcatccg ttaatgcgcg cggaatattt gctttctttg
    40621 cacggttttg atcttgccag cgagcagcat actgtgcgcg acaccgcgtt cctgatggaa
    40681 cagctggaac tgcgcgaaga actggacgag atcgaacagg cgaaagatga agcgcggctg
    40741 gaaagcttta tcaaacgtgt gaaaaagatg tttgataccc gccatcagtt gatggttgaa
    40801 cagttagaca acgagacgtg ggacgcggcg gcggataccg tgcgtaagct gcgttttctc
    40861 gataaactgc gaagcagtgc cgaacaactc gaagaaaaac tgctcgattt ttaatttctg
    40921 gaagctaaac atggccttat tacaaattag tgaacctggt ttgagtgctg cgccgcatca
    40981 gcgtcgtctg gcggccggta tcgaccttgg cacaaccaac tcgctggtgg cgacagtgcg
    41041 cagcggtcag gccgaaacgt tagccgacca tgaaggccgt cacctgctgc cgtctgttgt
    41101 tcactatcaa cagcaagggc attcggtggg ttatgatgcg cgtactaacg cagcgctcga
    41161 taccgccaac accattagtt ctgttaaacg cctgatgggg cgctcgctgg ctgatatcca
    41221 gcaacgctat ccacatctgc cttatcaatt ccaggccagc gaaaacggcc tgccgatgat
    41281 tgaaacggcg gcggggctgc tgaacccggt gcgcgtttct gcggacatcc tcaaagcact
    41341 ggcggcgcgg gcaactgaag ccctggcagg cgagctggat ggtgtagtta tcaccgttcc
    41401 ggcgtacttt gacgatgccc agcgtcaggg caccaaagac gcggcgcgtc tggcggggtt
    41461 gcatgttctg cgcttactta acgaaccgac cgctgcggct atcgcctacg ggctggattc
    41521 cggtcaggaa ggcgtgatcg ccgtttatga cctcggcggc gggacgtttg atatttccat
    41581 tctgcgctta agtcgcggcg tgtttgaagt gctggcgacc ggcggtgatt ccgcgctcgg
    41641 cggcgatgat ttcgaccatc tgctggcgga ttacattcgc gagcaggcgg gcattcctga
    41701 tcgtagcgat aaccgcgttc agcgtgaact gctggatgcc gccattgcag ccaaaatcgc
    41761 gctgagcgat gcggactccg tgactgtgaa tgttgcgggc tggcagggcg aaatcagccg
    41821 tgaacaattc aacgaactga tcgcgccact ggtaaaacga accttgctgg cttgtcgtcg
    41881 cgcgctgaaa gacgctggcg tagaagctga tgaagtgctg gaagtggtga tggttggcgg
    41941 ttctactcgc gtgccgctag tgcgtgaacg agtaggcgaa tttttcggtc gtccaccgct
    42001 gacttctatc gacccggata aagtcgtcgc tattggcgcg gcgattcagg cggatattct
    42061 ggtgggtaac aagccggaca gcgaaatgct gttgctggat gtgatcccgc tgtcgctggg
    42121 cctcgaaacg atgggcggcc tggtggagaa agtgattccg cgtaatacca ctattccggt
    42181 ggcgcgcgct caggatttca ccacctttaa agatggtcag acggcgatgt ctatccatgt
    42241 aatgcagggt gagcgcgaac tggtgcagga ctgccgctcg ctggcgcgtt ttgcgctgcg
    42301 tggtattccg gcgttaccgg ctggcggcgc gcatattcgc gtgacgttcc aggtcgatgc
    42361 cgacggtctt ttgagcgtga cggcgatgga gaaatccacc ggcgttgagg catctattca
    42421 ggtcaaaccg tcctacggtc tgaccgacag cgaaatcgcc tcgatgatca aagactcaat
    42481 gagctatgcc gagcaggacg taaaagcccg tatgctggca gaacaaaaag tagaagcggc
    42541 gcgtgtgctg gaaagtttgc acggcgcgct ggctgctgat gccgcgctgt taagcaccgc
    42601 agagcgtcag gtcattgacg atgctgccgc tcacctgagt gaagtggcgc agggcgatga
    42661 tgttgacgcc atcgaacaag cgattaaaaa cgtagacaaa caaacccagg atttcgccgc
    42721 tcgccgcatg gaccagtcgg ttcgtcgtgc gctgaaaggc cattccgtgg acgaggttta
    42781 atatgccaaa gattgttatt ttgcctcatc aggatctctg ccctgatggc gctgttctgg
    42841 aagcgaatag tggtgaaacc attctcgacg ttgcgctgcg taacggtatc gagattgaac
    42901 acgcctgtga aaaatcctgt gcttgcacca cctgccactg catcgttcgc gaaggttttg
    42961 actcgctgcc ggaaagctca gagcaggaag acgacatgct ggacaaagcc tggggactgg
    43021 agccggaaag ccgtttaagc tgccaggcgc gcgttaccga cgaagattta gtggtcgaaa
    43081 tcccgcgtta cactatcaac catgcgcgtg agcattaaca gaggttagta tgggacttaa
    43141 gtggaccgat agccgcgaaa ttggcgaagc actgtacgat gcgtatcccg atcttgatcc
    43201 gaaaacggtt cgattcaccg atatgcatca gtggatttgc gatctggaag atttcgacga
    43261 cgacccgcag gcatcgaacg agaaaatcct cgaagcgatt ttgttggtct ggctggacga
    43321 agccgagtga atgaacgggc tgcctggggg tagcccgttt tgctgttaaa cgaataagga
    43381 taactaaaat gacagaagcg atgaaaatta ccctttctac ccaacctgcc gacgcgcgct
    43441 ggggagaaaa agcgacttac agcattaata atgatggcat taccctgcat ttgaacggga
    43501 cagacgatct ggggctgatc cagcgtgcgg cgcgcaagat tgacggtctg ggcatcaagc
    43561 atgttcagtt aagcggtgaa gactgggatg cggatcgctg ctgggcattc tggcaaggtt
    43621 acaaagcccc gaaaggcaca cgtaaagtgg agtggccgga tctggacgat gcccaacggc
    43681 aggaactgga taaccgcctg atgatcatcg actgggtgcg tgacaccatc aacgcaccgg
    43741 cggaagaact ggggccatcg caactggcac agcgtgctgt tgatctgatc agcaacgtcg
    43801 cgggcgatcg tgtgacttac cggatcacca aaggcgaaga tctgcgtgat caaggttata
    43861 tggggctgca cacggtcggg cgcggttcag aacgttcccc ggtattgctg gcgctggatt
    43921 acaacccaac aggcgataaa gaagcgccag tgtacgcgtg cctggttggt aaaggcatca
    43981 cctttgattc cggcggctac agtatcaaac agaccgcatt tatggactcg atgaagtcgg
    44041 acatgggcgg cgcggcaacg gttaccggag cgctggcgtt tgccattacg cgcggactga
    44101 acaagcgcgt gaagctgttc ctctgctgtg cggataacct gatcagcggc aatgcgttca
    44161 agctgggcga tatcattacc tatcgcaacg gtaaaaaagt tgaagtgatg aacaccgatg
    44221 cggaagggcg tctggtgctg gccgatggcc tgattgatgc cagtgcgcag aaaccggaac
    44281 tgatcattga tgcggcgacc ctcaccgggg cggcgaaaac cgcgctgggt aatgattatc
    44341 acgcgctgtt cagttttgac gatgcgcttg ccgctcgctt gctggcgagt gccgcgcagg
    44401 agaatgaacc attctggcgt ctgccgctgg cggagttcca ccgcagccag ctgccgtcta
    44461 actttgccga actgaacaat accggaagcg cggcgtatcc ggcaggtgca agcacggcgg
    44521 cgggcttcct gtcgcacttt gttgagaact atcagcaagg ctggctgcat atcgactgct
    44581 cggcgactta ccgtaaagcg ccggttgaac agtggtctgc gggcgcgacc gggctaggtg
    44641 tgcgcacgat tgctaatctg ttaacggcgt aaacgtctta tttggcctac aaaattgcga
    44701 aaatccaata gattgagatt cccttgtagg cctgataagc gtagcgcatc aggcacttgt
    44761 gccccgaacg ttgaatgaat tgtggttctt atgtccgaaa caaaaaacga acttgaagac
    44821 ctgctggaaa aagcagcaac cgaaccggcg caccgcccgg cctttttccg tactctactg
    44881 gaatccaccg tctgggtgcc gggtacggcg gcgcagggcg aggctgtggt tgaagatagc
    44941 gcgcttgatt tacagcactg ggaaaaagaa gacggcacca gcgtcattcc tttttttacc
    45001 tcgttagaag cacttcaaca ggcggttgaa gacgaacagg catttgtcgt aatgcccgtt
    45061 cgcacattgt ttgagatgac ccttggcgaa acgctcttcc ttaatgccaa actgccaacc
    45121 ggtaaagaat ttatgccgcg tgaaatcagt ttgctgattg gtgaagaagg aaatccgctg
    45181 agcagccagg aagtactgga aggcggtgaa tcgctgatat tatcggaagt cgcagagcca
    45241 ccagcacaaa tgattgattc actcaccacc ttatttaaaa ctattaagcc ggtgaagcgt
    45301 gcttttattt gttcaattaa agagaacgaa gaggcacagc ctaatttact tattggcatt
    45361 gaagccgatg gtgatatcga agaaattatt caggcggcgg gaagtgtagc gaccgataca
    45421 ttacctggcg atgaacccat cgatatttgt caggtgaaaa aaggggaaaa aggaattagc
    45481 cactttatta ccgaacatat tgcgccattc tatgaacgtc gctggggtgg ttttttgcgt
    45541 gactttaaac agaaccggat aatctaaaat atgccgcccc aaagggcggc attcgggctt
    45601 acttgctgct ctgaaggttg ttaaccgcca gtaaattgtt gccggagaga ctccggttat
    45661 tatttacctc gcttaacagc gcaccagaaa caataagtac gatacaaaag caccgtatct
    45721 tcattgtgcg gtccatgcga agaccgcatc acgacagcac cactggcgat attgccgcga
    45781 tagcaagcgc tgagacgcgc ttgctctaac ggtcgcggaa catcctttcc ttaagccagc
    45841 gcccgcttgc gagacggcgg aaaggtccgc agtggataat acactaacag aacaataata
    45901 actctctttt acttgacaaa aagagagtta ctggcgagta ttgtgtccgc tgagacgcgc
    45961 ttgcattact acggtaatac aatctgctgg aaaggccgcg aaccaaacca gcaacaaata
    46021 accgcctgat tttgg
//
LOCUS       DAZGQL010000029        45657 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.029, whole genome shotgun sequence.
ACCESSION   DAZGQL010000029 DAZGQL010000000
VERSION     DAZGQL010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 45657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45657
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.029"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            39..1589
                     /locus_tag="ACHKIR_001878"
     CDS             39..1589
                     /locus_tag="ACHKIR_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFO9989627.1"
                     /translation="MKTVFTTLSVIFGVIFSHSVLAQDVTIYYTNDIHAHVNPAKIIA
                     VDKNRLIGGMANISGIVNEAKKKSKDVFFFDAGDYFTGPYISTLTKGEAIIDIMNTMP
                     FDAVSVGNHEFDHGVPNMVSQLSKAKFPILLGNIYYTDTNKPVWDHPWTIIEKDGLKI
                     GVIGLHGAFAFYDTVAAKAREGVEARDEIKYLNKALAELKGKVDITVLLIHEGVPARQ
                     SSFGSKDVERLLQADIETAKKVNGVDVLITGHAHVGTPQPIKVNNTLIVSTDAYGTNI
                     GKLVLDYNPKTKKIDSYNGELITIFADQFKPDTIVQNTIDKWSAKLNKITQEVVGHSP
                     VVLTREYGSSSSTGNLILDAMMEKTPDAVAGFQNSGGIRADFPKGDITLGDVISTFPF
                     NNDLIEMDLTGRDLKSLMTHATNLTNGVLQVSKSVAVVYDSKKPLNQRLISFTINGKP
                     VEDNQTYRIATHSFCASGGDGFEAFLNGKNVKTIPGTTSAESIIDYFKNHKPVTPDLT
                     KRVMDVAK"
     gene            1630..3249
                     /locus_tag="ACHKIR_001879"
     CDS             1630..3249
                     /locus_tag="ACHKIR_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFO9989628.1"
                     /translation="MKTAQRTLFAATLLTIFSTSVMAQDVTIIYTNDLHAHVDSYKVP
                     YVADGKRDIGGFANISTLVKQEKAKNKATFYFDAGDYFTGPYISSLTKGEAIIDIMNT
                     MPFDAVSIGNHEFDHGWDNALRQLSKANFPVLLANVYHKESEKPFWNKPYTILEKDGI
                     KIGIVGLHGVFAFNDTVSELSLQGLDNDNNNRFDKSSATLKNQGIEARDEVKYLQHYI
                     DELRDKVDLTVALVHEGVPARQSSIGNTDVRRALDKDIQTASKVKGLDILITGHAHVG
                     TPEPIKVGNTLILSTDSGGIDIGKLILDVNPTARTHKVKSFELKTVYADEWIPDPTTQ
                     KVINGWNKKLADLVRQPVGESSIALTRAYGESSQLGNLFTDAMLVAAPTAQIALINSG
                     SLRADINAGTITFGDITSTFPFKNELTEMDLSGKDLRNLLEHGASLTNGILQMSKGAE
                     MRYTPKKPVGQRIVSFKINGEEIVDTNIYHVATTTFLALGGDGFLAFKEGKNVQVRAG
                     NNMSDVVIDYLKKGHKITPAQVNEMRVDVSK"
     gene            3328..4188
                     /locus_tag="ACHKIR_001880"
     CDS             3328..4188
                     /locus_tag="ACHKIR_001880"
                     /inference="COORDINATES: protein motif:HMM:NF011686.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HFO9989629.1"
                     /translation="MCIKKHGIALSILLIPCIGNAQEIKIDESWLHQSLNVIGRTDSR
                     FGPRLTNDLYPEYTVAGRKDWFDFYGYVDLPKFFGVGSHYDVGIWDEGSPLFTEIEPR
                     FSIDKLTGLNLAFGPFKEWFIANNYVYDMGDNQSSRQSTWYMGLGTDIDTGLPIKLSA
                     NIYAKYQWQNYGAANENEWDGYRFKIKYSIPLTHLFGGRLVYNSFTNFDFGSDLADKS
                     HNNKRTSNAIASSHILSLLYEHWKFAFTLRYFHNGGQWNAGEKVNFGDGPFELKNTGW
                     GTYTTIGYQF"
     gene            complement(4405..6837)
                     /gene="metL"
                     /locus_tag="ACHKIR_001881"
     CDS             complement(4405..6837)
                     /gene="metL"
                     /locus_tag="ACHKIR_001881"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HFO9989630.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPTHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(6840..8000)
                     /gene="metB"
                     /locus_tag="ACHKIR_001882"
     CDS             complement(6840..8000)
                     /gene="metB"
                     /locus_tag="ACHKIR_001882"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFO9989631.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRVALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            8277..8594
                     /gene="metJ"
                     /locus_tag="ACHKIR_001883"
     CDS             8277..8594
                     /gene="metJ"
                     /locus_tag="ACHKIR_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HFO9989632.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            8654..9262
                     /gene="yiiX"
                     /locus_tag="ACHKIR_001884"
     CDS             8654..9262
                     /gene="yiiX"
                     /locus_tag="ACHKIR_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HFO9989633.1"
                     /translation="MKIRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVIRNKKPYVFEAVGPVKYTPLKQWIAHGEKGKYVVRRVEGELSVEQQQK
                     LAQTAKRYLGKPYDSSFSWSDDRQYCSEVVWKVYQNALGMRVGKQQKLKEFDLSNPQV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(9323..9535)
                     /gene="rpmE"
                     /locus_tag="ACHKIR_001885"
     CDS             complement(9323..9535)
                     /gene="rpmE"
                     /locus_tag="ACHKIR_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFO9989634.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            9738..11936
                     /gene="priA"
                     /locus_tag="ACHKIR_001886"
     CDS             9738..11936
                     /gene="priA"
                     /locus_tag="ACHKIR_001886"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFO9989635.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     VVVSVSNVSELPLNELKAVVEVLDVEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRKNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQADNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPGVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIINGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            12092..13117
                     /gene="cytR"
                     /locus_tag="ACHKIR_001887"
     CDS             12092..13117
                     /gene="cytR"
                     /locus_tag="ACHKIR_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HFO9989636.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHNRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            13209..14168
                     /gene="ftsN"
                     /locus_tag="ACHKIR_001888"
     CDS             13209..14168
                     /gene="ftsN"
                     /locus_tag="ACHKIR_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFO9989637.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGMRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKIAGHTNCIRLAAGG"
     gene            14261..14791
                     /gene="hslV"
                     /locus_tag="ACHKIR_001889"
     CDS             14261..14791
                     /gene="hslV"
                     /locus_tag="ACHKIR_001889"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HFO9989638.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            14801..16132
                     /gene="hslU"
                     /locus_tag="ACHKIR_001890"
     CDS             14801..16132
                     /gene="hslU"
                     /locus_tag="ACHKIR_001890"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HFO9989639.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQTITIDADYVSKHLDALVADEDLSRFIL"
     gene            16199..17125
                     /gene="menA"
                     /locus_tag="ACHKIR_001891"
     CDS             16199..17125
                     /gene="menA"
                     /locus_tag="ACHKIR_001891"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFO9989640.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGAVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            17218..17703
                     /gene="rraA"
                     /locus_tag="ACHKIR_001892"
     CDS             17218..17703
                     /gene="rraA"
                     /locus_tag="ACHKIR_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HFO9989641.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(17788..18033)
                     /gene="zapB"
                     /locus_tag="ACHKIR_001893"
     CDS             complement(17788..18033)
                     /gene="zapB"
                     /locus_tag="ACHKIR_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HFO9989642.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            18459..19304
                     /gene="glpF"
                     /locus_tag="ACHKIR_001894"
     CDS             18459..19304
                     /gene="glpF"
                     /locus_tag="ACHKIR_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HFO9989643.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            19327..20835
                     /gene="glpK"
                     /locus_tag="ACHKIR_001895"
     CDS             19327..20835
                     /gene="glpK"
                     /locus_tag="ACHKIR_001895"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFO9989644.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            21006..22016
                     /gene="glpX"
                     /locus_tag="ACHKIR_001896"
     CDS             21006..22016
                     /gene="glpX"
                     /locus_tag="ACHKIR_001896"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFO9989645.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            22113..22859
                     /gene="fpr"
                     /locus_tag="ACHKIR_001897"
     CDS             22113..22859
                     /gene="fpr"
                     /locus_tag="ACHKIR_001897"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HFO9989646.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEIE
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLERFKNMVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRLPALIESGELESAVGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(22864..23292)
                     /gene="uspD"
                     /locus_tag="ACHKIR_001898"
     CDS             complement(22864..23292)
                     /gene="uspD"
                     /locus_tag="ACHKIR_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HFO9989647.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKLSADLLIVPFIDK"
     gene            complement(23319..23618)
                     /gene="yiiS"
                     /locus_tag="ACHKIR_001899"
     CDS             complement(23319..23618)
                     /gene="yiiS"
                     /locus_tag="ACHKIR_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HFO9989648.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(23830..24270)
                     /gene="yiiR"
                     /locus_tag="ACHKIR_001900"
     CDS             complement(23830..24270)
                     /gene="yiiR"
                     /locus_tag="ACHKIR_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFO9989649.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            24371..24970
                     /gene="yiiQ"
                     /locus_tag="ACHKIR_001901"
     CDS             24371..24970
                     /gene="yiiQ"
                     /locus_tag="ACHKIR_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HFO9989650.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQAYMAAVIRTLTPLMTKTQSQKKLQALLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            25078..25845
                     /gene="tpiA"
                     /locus_tag="ACHKIR_001902"
     CDS             25078..25845
                     /gene="tpiA"
                     /locus_tag="ACHKIR_001902"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFO9989651.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(25900..26655)
                     /gene="cdh"
                     /locus_tag="ACHKIR_001903"
     CDS             complement(25900..26655)
                     /gene="cdh"
                     /locus_tag="ACHKIR_001903"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008715 - CDP-diacylglycerol diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HFO9989652.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(26761..27750)
                     /gene="sbp"
                     /locus_tag="ACHKIR_001904"
     CDS             complement(26761..27750)
                     /gene="sbp"
                     /locus_tag="ACHKIR_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HFO9989653.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKVFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(28070..29032)
                     /gene="pfkA"
                     /locus_tag="ACHKIR_001905"
     CDS             complement(28070..29032)
                     /gene="pfkA"
                     /locus_tag="ACHKIR_001905"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFO9989654.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIELL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(29213..30115)
                     /gene="fieF"
                     /locus_tag="ACHKIR_001906"
     CDS             complement(29213..30115)
                     /gene="fieF"
                     /locus_tag="ACHKIR_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312867.1"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HFO9989655.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLISPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(30264..30764)
                     /gene="cpxP"
                     /locus_tag="ACHKIR_001907"
     CDS             complement(30264..30764)
                     /gene="cpxP"
                     /locus_tag="ACHKIR_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HFO9989656.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            30914..31612
                     /gene="cpxR"
                     /locus_tag="ACHKIR_001908"
     CDS             30914..31612
                     /gene="cpxR"
                     /locus_tag="ACHKIR_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HFO9989657.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALSLNPGRQEATFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            31609..32982
                     /gene="cpxA"
                     /locus_tag="ACHKIR_001909"
     CDS             31609..32982
                     /gene="cpxA"
                     /locus_tag="ACHKIR_001909"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HFO9989658.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(33088..33762)
                     /gene="yiiM"
                     /locus_tag="ACHKIR_001910"
     CDS             complement(33088..33762)
                     /gene="yiiM"
                     /locus_tag="ACHKIR_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxyaminopurine reductase"
                     /protein_id="HFO9989659.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTDLGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWAREFPEQAELFVAPAFGENLSTDGLTESNVYIGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGLVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGVSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(33911..34894)
                     /gene="kdgT"
                     /locus_tag="ACHKIR_001911"
     CDS             complement(33911..34894)
                     /gene="kdgT"
                     /locus_tag="ACHKIR_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015649 - 2-keto-3-deoxygluconate:proton
                     symporter activity [Evidence IEA];
                     GO_process: GO:0046411 - 2-keto-3-deoxygluconate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HFO9989660.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPEAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(35154..35774)
                     /gene="sodA"
                     /locus_tag="ACHKIR_001912"
     CDS             complement(35154..35774)
                     /gene="sodA"
                     /locus_tag="ACHKIR_001912"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HFO9989661.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            36059..37093
                     /gene="rhaT"
                     /locus_tag="ACHKIR_001913"
     CDS             36059..37093
                     /gene="rhaT"
                     /locus_tag="ACHKIR_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HFO9989662.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWAISALLLPNFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLINTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     VIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLIIHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNAGRRPVTVLSLGCVVIIVA
                     ANIVGMGMAN"
     gene            complement(37090..37938)
                     /gene="rhaR"
                     /locus_tag="ACHKIR_001914"
     CDS             complement(37090..37938)
                     /gene="rhaR"
                     /locus_tag="ACHKIR_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HFO9989663.1"
                     /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIV
                     WRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQG
                     AIPGFSASAGQPHWRLGSVGMAQARQIIGQLEHESSQHVPFANEMAELLFGQLVMLLN
                     RHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTG
                     MTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRH
                     LNSQKD"
     gene            complement(38012..38848)
                     /gene="rhaS"
                     /locus_tag="ACHKIR_001915"
     CDS             complement(38012..38848)
                     /gene="rhaS"
                     /locus_tag="ACHKIR_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HFO9989664.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQEQDGQYPSHWRVNHSVLQQVRQLVAQMEQQEEENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARYLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            39136..40605
                     /gene="rhaB"
                     /locus_tag="ACHKIR_001916"
     CDS             39136..40605
                     /gene="rhaB"
                     /locus_tag="ACHKIR_001916"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFO9989665.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTHGLMAQAQRQLGKCDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQSNEIPVVAVASHDTASAVIASPLNSSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFTINP
                     NDDRFINPDEMCSEIQAACRETAQPIPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNALLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            40602..41861
                     /gene="rhaA"
                     /locus_tag="ACHKIR_001917"
     CDS             40602..41861
                     /gene="rhaA"
                     /locus_tag="ACHKIR_001917"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFO9989666.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVARDQIKPEHFKNWVEWAKTNQLGLDFNPSCFSHPLSADGFTLSHADDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLDP
                     AHHIDAVESKLFGIGAESYTVGSNEFYLGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLESAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLENVRAYEKEILSRRG"
     gene            42046..42870
                     /gene="rhaD"
                     /locus_tag="ACHKIR_001918"
     CDS             42046..42870
                     /gene="rhaD"
                     /locus_tag="ACHKIR_001918"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFO9989667.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAP
                     YHDNFHQQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGIVKVDSDGAGY
                     HILWGLTNKAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     PTLDETFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGQRFGVTPLASALAL"
     gene            42880..43194
                     /gene="rhaM"
                     /locus_tag="ACHKIR_001919"
     CDS             42880..43194
                     /gene="rhaM"
                     /locus_tag="ACHKIR_001919"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFO9989668.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASTDVCQRWWKYMTDVMPTNPDNSPVSSELQEVFY
                     LP"
     gene            complement(43235..44629)
                     /locus_tag="ACHKIR_001920"
     CDS             complement(43235..44629)
                     /locus_tag="ACHKIR_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HFO9989669.1"
                     /translation="MKKSTLSLAIGLLLACSTGMAKTQHLTLEQRMALLEERLEAAEM
                     RAAKAESQVKQLQTQQAAEIREIKAAQGNTPVNGQATSESAKKNSTSPNLLLSGYGDL
                     KIYGDVEFNMDAESNHGLLAMTNADVNSDPTNEQWNLNGRILLGFDGMRKMDNGYFAG
                     FSAQPLGDMHGSVNIDDAVFFFGKENDWKVKVGRFEAYDMFPLNQDTFVEHSGNTAND
                     LYDDGSGYIYMMKEGRGRSNAGGNFLVSKQLDNWYFELNTLLEDGTSLYNDGNYHGRD
                     MEQQKNVAYLRPVIAWSPTEEFTVSAAMEANVVNNAYGYTDSKGNFVDQSDRTGYGMS
                     MTWNGLKTDPENGVVVNLNTAYLDANNEKDFTAGINALWKRFELGYIYAHNKIDEFSG
                     VVCDNDCWIDDEGTYTIHTIHASYQFANVMDMENFNIYLGTYYSILDSDGDKKHGDDT
                     DDRYGARVRFKYFF"
     gene            complement(44983..45657)
                     /locus_tag="ACHKIR_001921"
                     /pseudo
     CDS             complement(44983..45657)
                     /locus_tag="ACHKIR_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418332.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="YiiG family protein"
BASE COUNT        11491 a        11723 c        11453 g        10990 t
ORIGIN      
        1 aattcaacaa atgtaacaca ccctatccag gagggatgat gaaaactgta tttacaacat
       61 tatcagtcat attcggcgtg atattttctc acagtgttct agctcaggac gttactattt
      121 attatacgaa tgacattcac gcacatgtca atccagcaaa aatcatcgct gttgataaga
      181 acagacttat aggtggtatg gctaatattt caggcatcgt taatgaagcg aagaaaaaaa
      241 gcaaagatgt gtttttcttt gatgcaggcg actattttac cgggccgtat atcagcaccc
      301 tgaccaaagg tgaggctatt attgatatca tgaacactat gccctttgat gcggtgtctg
      361 ttggtaatca cgagttcgat cacggtgtgc ccaatatggt gtctcagtta tcaaaagcaa
      421 aattccccat tctgctggga aatatctact acaccgatac gaataaacca gtttgggatc
      481 atccgtggac cattatcgaa aaagatggtt taaagattgg tgtgattgga ttgcatggcg
      541 cattcgcttt ctatgataca gtcgcggcaa aagcacgtga aggcgtagaa gccagagacg
      601 aaattaaata tttaaataaa gcacttgcag aattaaaagg taaagtcgac attaccgttt
      661 tgctaattca cgaaggtgtc ccggcgcgtc aatcaagctt tggtagtaaa gatgtggagc
      721 ggctactaca ggcagatatt gagacagcta aaaaagtgaa tggtgttgat gtcttaatta
      781 ccggtcacgc acatgtcgga acaccgcaac caataaaagt taataacaca ttaattgtat
      841 ctaccgatgc atacggcacc aacatcggaa aacttgtgct tgattacaat ccgaaaacga
      901 agaaaattga cagttataat ggtgagttaa tcaccatctt tgcagatcaa tttaaacctg
      961 acaccatcgt tcaaaatact attgataaat ggagtgcaaa gcttaacaaa ataacacagg
     1021 aagttgtcgg ccattctccc gtagttttaa cacgtgagta tggtagttcc tcttctaccg
     1081 gcaaccttat tcttgatgca atgatggaaa aaacacctga tgccgttgcc ggatttcaaa
     1141 atagcggtgg gattcgagct gattttccta aaggcgatat cacactggga gatgttatta
     1201 gcacattccc ctttaataat gacctcatcg agatggattt gacgggccgc gatctcaaat
     1261 cattgatgac gcatgcaacc aatctaacta acggtgtgtt acaagtttct aaaagtgttg
     1321 cggttgtcta tgacagcaaa aaacccctca accaacggtt aatctctttc accattaacg
     1381 gcaaacccgt ggaagataat caaacatatc gtattgccac gcactccttt tgtgccagtg
     1441 gtggtgacgg ttttgaagca tttttgaatg gaaaaaatgt gaagacgata ccgggaacaa
     1501 cctcggcgga atctatcatc gattatttca aaaatcataa acctgtcacc ccagacttaa
     1561 ctaaacgagt catggacgtc gccaaataaa atctcattta tcagtcaatt tatttaacaa
     1621 ggaatgcgaa tgaaaacagc gcagcgtact ttatttgccg caacattgct gactattttt
     1681 tcgacctcag ttatggcgca ggatgtcacg attatttata ccaatgatct tcacgctcat
     1741 gtggattcct ataaagtacc gtatgtcgca gacggaaaac gcgacattgg tggctttgcc
     1801 aatatatcca cgttagtaaa acaagagaaa gccaaaaata aagccacctt ttattttgat
     1861 gctggagact atttcaccgg gccatatatc agtagcctga caaagggcga agcaataatt
     1921 gatattatga ataccatgcc ttttgacgca gtatcaatcg gtaatcatga gtttgaccat
     1981 ggctgggata atgccctccg acaattaagc aaagcgaatt tccctgtttt attagccaat
     2041 gtttatcata aagagagtga gaaacccttc tggaacaagc cctacaccat cctggaaaaa
     2101 gatggcatca agattggtat tgttgggcta cacggcgtat ttgcatttaa tgacacggtc
     2161 tcggagctct ctctccaggg actcgataat gacaataata accgatttga taaatcttcc
     2221 gcgaccctca agaatcaggg gattgaagca cgtgatgaag tgaagtattt gcagcattat
     2281 atcgatgagt tacgtgataa ggttgacctc accgtggcgc tggttcatga aggcgtaccg
     2341 gcgcgtcagt ccagcattgg caatacagat gttagacgcg ctcttgataa agatattcaa
     2401 accgcaagca aggtgaaagg attggatatt ctgattaccg ggcatgccca cgtcggaacg
     2461 ccagaaccaa tcaaagtcgg caacacctta atcctttcga ctgacagtgg cggtatcgat
     2521 attggtaaat taatccttga tgtcaaccct acagctcgta ctcataaagt gaagagcttt
     2581 gagctgaaaa cggtttatgc cgatgaatgg atacctgatc caaccacaca aaaagttatc
     2641 aatggctgga ataaaaaact ggcggatctt gtacgccaac cagtcggtga atcatcgatt
     2701 gcattaactc gtgcctacgg tgagtcatcg caacttggca acctgtttac cgatgcaatg
     2761 cttgtcgctg caccgactgc acagattgca ttgataaact ccggcagctt acgtgcggat
     2821 ataaatgcag gcacgattac ctttggcgac attaccagta cattcccctt caagaatgaa
     2881 cttactgaaa tggatctcag cggtaaggat ctgcgtaacc tactggaaca cggggcatcg
     2941 cttaccaatg gcatcctgca aatgtcaaaa ggcgcagaaa tgcgctatac cccgaagaaa
     3001 ccagtaggtc agcgcatagt atcttttaaa attaatggtg aagagatcgt tgatacgaat
     3061 atttaccatg ttgcaacaac gactttcctt gcactggggg gcgatggttt tctggcattc
     3121 aaagaaggga aaaatgttca ggtccgcgcc ggaaataaca tgtccgatgt cgtgatcgat
     3181 tatttgaaga aaggccacaa gattacgcct gcacaggtaa atgaaatgcg ggtggacgtt
     3241 agcaaataat aaaaaaatta taatgccatt cattcactga atggcattat ttttctctaa
     3301 atcaacaata aataatgaga tatatttatg tgtataaaaa agcacgggat agctttatcc
     3361 attctattaa taccttgcat tggaaacgct caggaaatta aaattgatga aagctggtta
     3421 catcaaagct tgaatgtcat tggtcgcaca gactctcgct ttggcccaag actgactaac
     3481 gacctctacc ctgaatatac tgtagcagga agaaaagact ggtttgattt ttatggttat
     3541 gttgatctac caaaattctt tggcgtcggc agtcactatg atgttgggat ctgggatgag
     3601 ggctcaccac tatttacgga aatagaacct cggttttcca ttgacaaatt gaccggatta
     3661 aatcttgcgt tcggcccttt taaagaatgg ttcattgcaa acaactatgt ctatgatatg
     3721 ggtgacaacc agtcatcccg gcaaagtaca tggtatatgg ggcttggtac agatatcgac
     3781 acgggtctac caattaagct ttctgccaat atatacgcca agtatcagtg gcaaaactat
     3841 ggtgccgcta atgaaaatga atgggacgga tatcgattca aaataaaata tagcattcct
     3901 cttacccatt tattcggagg gcgattggta tacaatagtt ttactaactt tgattttggc
     3961 tccgatcttg cggacaaatc acacaataat aaacgaacca gtaatgctat tgcttcaagc
     4021 catatccttt ccctgctata tgaacactgg aaatttgcat ttacactacg ttattttcac
     4081 aacggtggac aatggaatgc tggggagaaa gttaacttcg gagatggtcc atttgaatta
     4141 aaaaacacag gatggggaac ctatactact attggttatc aattttaaat caggtgatgt
     4201 tttagatatt tttacttaat gccattttgt aattgtcata aataacttca ccattcacac
     4261 tgtgaattca tcgctcaaca caaaagcccc cttattaccg gggcactgac caaaggcaaa
     4321 atgacaatca atcaccacct ccgcaggagg tggtttagag gtgacaacaa aagctggcaa
     4381 ctgccagctt ttgttcaata atcactacaa caactgcgcc agacggttaa tatccgactg
     4441 aatcgccccg gcggtgacgt cgcgccctgc acctggcccg cggatcacca acggattatc
     4501 gcgataccag cggctttcga tggcaaagac gttatcgcac ggtagtagtg atgccagcgg
     4561 atggtcttct cgcaccgctt ccacgcctac tcgcgctttg ccgttggcat cgaaacgcgc
     4621 cacgtagcgc aacaccagcc ccatttcacg ggccgcttcc aaccgttgta ccatctgctc
     4681 attaagttca tcgccatttt caaagaaatg gtcgatgctt ccaccttcgc aatgggttgg
     4741 caccagtgat tcaacacgca cctggtccgg ttcaatgttg taacccgctt cacgcgccag
     4801 aatcaccagc ttgcgcatca catctttgcc ggagaggtca tcacgcgggt caggttcagt
     4861 taagccctgc tgccacgcct gatccaccag ctcagtaaac ggcacgctac cgtcgaattg
     4921 caggaacagc caggagagcg tgccggagaa gatcccgctg atcgacaaaa tagtgtcgcc
     4981 gctgtcgatc agatcgcgca cggtatggtt gatcggcaaa cctgcgccga cggtggcgtt
     5041 gtacagccag tgacgcccgg ttttttcgaa cgcgtcgtgg atctggcgat atttattgct
     5101 gtcgctcgct cccgccagtt tgttggcact gatgacgtgg aaaccgtggc tggcgaaatc
     5161 gagatactga tcagcaagct gctggctggc agtaacgtcc agcacaacta aatcatcata
     5221 cggatgggca cgcatccaca ggaatagcga ctcttcatcc tgttcgacgg cttcatcatt
     5281 aaagaaggct agcgcacggc tggcgtccag cccgtcatag ctcaacaggc tgcgacggct
     5341 gtccaccacg cctgccagca caaattcaaa gccggtgcgc gccgagagcg tgctctgctc
     5401 acgggcgaac agttccagcc agcgggaacc gatatttccc ttaccgaaca ataccaggcc
     5461 gatgcgtttt tctgcgcgga agacggactg atgcagcccc tgaatcaggc tttcggtcgg
     5521 gccggtgcgc agtactgcca ccaggctgat gccgtcatcg gactgccagg taaactcgac
     5581 cggctggcct ttcagttgct gccagaagcg gtggcaatgc agcgggttac gggtgacgcc
     5641 cgccccaacc atcgccacca gcgccagccc ctgacgcaga cgcagttcgc cgggtaatcc
     5701 tgcttcgtcg aggattttca gcgcactgtc ggccacttcc gaggtgtagc aaaattgcag
     5761 caactggcga tcgttatgca cgccaactgc cagtgggcgc acctgagcac gtttcaggat
     5821 ttggtcgatc tctttatgcg ccagtttaaa atcctgactg gcgggcacct gaaactcaat
     5881 caaacagaca tcatcgtggc tggtgacaat acgcgcacca gtaccggagg ccagcacgcg
     5941 ttcaatgcgc gtggaacctt gatccggcgt gtagctacag cgcagctgca ggtcgatttc
     6001 actaccggat actggctgta aagtacgggc gtgaagaacg ggggccgcca gacgcgccag
     6061 ttcgctggct tcatcaagac gcagcaatgg cagcagacag gcatctttca ctttacgcgg
     6121 gtcggcacta tataccccgg cgacatcgct ccagatggtt acgcgggaaa cacccgccag
     6181 cgcaccgatt tgtgtcgcgg aatagtcgga accgttacgc cccagcagca ccgtttcacc
     6241 ggcgttgtta cggctgataa acccggtcac caccagacgt ttgcccggat gttgtaccag
     6301 tagctgttgc agcaacgggt aagaaagccc ttcatcaacc tgcggctgcg cggcgcgttc
     6361 agcgcgtaaa aactcgcggg catcaagcca ggccgctggc agcccttgtt gattaagtac
     6421 cgcagacatc aggcgcgccg accacacttc accgtggccc accacttccg catacaccgc
     6481 atcattaata ccgctgtcga gcagcgccgc gaggcgttcc agatcgctga caaaggcgct
     6541 aatgaggcta tcggcttctt cggcgggtag caggccgcta atcagatcgc actgataacg
     6601 acgtagcgtt tgttgaacct gatgcgcaga gagacgatcg gtctggctta gtttcaacca
     6661 gttaatcaac tggttagtgg tgctaccggc ggcggaaacc accatcatat cgtcaggctg
     6721 agagtactcc gccataatgc ccgcgacacg caaataacac ttcacatcag ccagactact
     6781 gccaccaaat ttatgcagtt gacgaccttt cgcccctgcc tgcgcaatca cactcatttt
     6841 taccccttgt ttgcagcccg gaagccattt tccaggtcgg caattaaatc ttcgccatct
     6901 tcaataccgg tggagatacg tagcagcgtc tcggagatcc cggcggcagc acgcgcttct
     6961 ggcgccatgc ctgcatgtgt catggttgcg gcgtgagaga ttaaactttc cactccccct
     7021 aatgattccg ccagcgtaaa caacgacaac ccgcccagga aacgacgcag cgtctgctca
     7081 tcgccatcca gttcaaaact caacattgcg ccaaagcctt tttgctggcg cgcggcaatt
     7141 tcatgcccct gattttccgg cagcgacggg tgatacagtt ttttcaccaa cggctgggtt
     7201 tgcagatatt tcacaatcgc ctgcgcgttg cgctgcgcca gctccatacg cggcaccagc
     7261 gttcgcaacc cgcgcagcag cagatagctg tcaaacgcgc cgcccgtcac gccaatattg
     7321 tttgcccacc aggcaagttc agtgacaacg tccgggtctt tagcaatcac tacgccggcc
     7381 accacgtctg agtgaccgtt cagatatttc gtacatgaat gcaacaccag atcggcacct
     7441 aatgccagcg gattttgtaa tgccgggctt aagaaggtgt tatccaccac gctcaccgcc
     7501 ccgacttccc ttgccagatg gcagattttc gcaatatcca cgacgcgtaa caatggatta
     7561 cttgggcttt ctaccagtac cagtttgggt ttttctgcca gcgctacccg taatgcctgt
     7621 tcatcgcctt gatcaacaaa caacacgcga tagcaaccgc gtttcgccag actgtcgaac
     7681 aggcgatagc tgccgccata gcagtcgtgc ggcgcaacca gcagatcgcc cggtttcaaa
     7741 aagacggtcg ttaccagatg aatcgcggac atgccggtat tagtaagtac ggcaccagca
     7801 ccgccttcca gttctgccag cgcacgctga accacatcgc gcgttgggtt gccgcgccgc
     7861 gagtaatcat gcgcgcgcgg ttcattaaat ccggtaaagt tataggtgct ggaaagatgg
     7921 atcggtggga caacgcaacc atactgttcg tcgtcattta acccgctacg cactgcgatg
     7981 gtggcctgtt tacgcgtcat gtgatgaagt tccctgggct tggtcggtga aatgtcaggc
     8041 accagagtaa acattgtgtt aatggacgtc aatacatctg gacatctaaa cttctttgcg
     8101 tatagattga gcaaatccca aatagccgtt aaaattatat gcattatcac gccgacaggt
     8161 gcattacacg atgtcacggt agcgcctgta cggtaaacta tgcgggttta cggtcagtac
     8221 ccacataaat tgtgtggtct ggtctcaatt tattgacgaa gaggattaag tatctcatgg
     8281 ctgaatggag cggcgaatat atcagcccat acgctgagca cggcaagaag agtgaacaag
     8341 tcaaaaagat tacggtttcc attcctctta aggtgttaaa aatcctcacc gatgaacgca
     8401 cgcgtcgtca ggtgaacaac ctgcgtcacg ctaccaacag cgagctgctg tgcgaagcgt
     8461 ttctgcatgc ctttaccggg caacctttgc cggatgatgc cgacctgcgt aaagagcgca
     8521 gcgacgaaat cccggaagcg gcaaaagaga tcatgcgtga gatggggatt aacccggaga
     8581 cgtgggaata ctaaagcgga aaagagcggc gcggagttaa ccagccgctt ttttcataca
     8641 tggacgttta acgatgaaaa tcaggctact gatcctcagc ctgctggttt ctgtgcctgc
     8701 ctttgcctgg cagccgcaaa ctggcgacat catctttcaa atctctcgct catcgcaaag
     8761 taaagcgatc caactggcga cccattccga ttacagccac accggtatgc tggtgatacg
     8821 caacaaaaag ccctacgttt ttgaagcggt cggcccggta aaatacaccc cactcaagca
     8881 gtggatcgcc catggtgaaa agggcaaata cgttgttcgc cgcgttgaag gcgaactgag
     8941 tgttgaacaa cagcaaaaac tggcgcaaac ggcaaaacgt tatctcggta aaccgtatga
     9001 ctccagcttc tcgtggagcg acgatcgcca gtactgttcg gaagtggttt ggaaggttta
     9061 tcagaacgcg ctgggaatgc gcgtgggcaa acagcaaaag ttgaaagagt tcgaccttag
     9121 taacccgcag gttcaggcaa agctcaaaga acgctacggc aaaaatattc cgcttgagga
     9181 aacggtggtc tcgccacaag ccgttttcga tgcgccacaa ctcaccacgg tcgccaaaga
     9241 atggccgctg ttttcgtggt aatacgacca aaattcaggc acaaaaaaag cgccgtgcgg
     9301 cgcttttttc ggaaatccgg tcttatttgc tgcccgggat gttgaaacgc ttgttgaagc
     9361 ggtcaacacg gccaccggta gcaacatcac gctgtttgcc agtgaagaac gggtggcact
     9421 tgctgcacac gtcgaggttc aggtcatgac caacggtgga gcggattttc attacgttac
     9481 cgcaagagca gctagcagta atttcttcgt atttcgggtg aatatctttt ttcatgggaa
     9541 aacctcggtt taaggccgcg tcgctcttcc agccctaacg ccagacacca cgcgatgtta
     9601 aaagtatagc ttcaatacga tcatttcgta tgaaggcgcg aaatcataca gaaattaacc
     9661 agcgtatgca aactgatccg cactcttcta cggcaatgtg tatactaacc caccgaattt
     9721 caagtcagga tgatgctatg cccgttgccc acgttgcctt gcccgttccg cttccccgta
     9781 cctttgacta tctgctgcca gaaggcatga cggtcaaagc cgggtgtcgc gtgcgcgtgc
     9841 cgtttggcaa acagcaggag cgcatcgggg ttgtggtgtc agttagcaat gtcagcgagc
     9901 tgccgcttaa cgagctgaaa gcggtagttg aagtgctgga cgttgagccg gtatttactc
     9961 actccgtctg gcgattactg ctctgggcgg cagattacta tcatcatccg attggcgatg
    10021 tgctgtttca tgccttgccg attttactac gccaggggcg gcctgcggcg aacgcgccga
    10081 tgtggtactg gtttgccact gaacaaggtc aggcggtgga tctgaacagc ctgaaacgct
    10141 cacccaagca acaacaggcg ctggcagcgt tacggcaagg caaaatctgg cgcgaccagg
    10201 ttgccacgct cgaatttaat gatgccgcgt tgcaggcgct gcgcaaaaaa ggtctgtgtg
    10261 atttagcaag tgaaacacca gagtttagtg actggcgaaa gaattatgcc gtttctggtg
    10321 agcggttgcg attgaatact gaacaggcca ccgccgttgg cgcaattcat agtgcggcgg
    10381 ataccttctc tgcctggctg ctggcgggcg ttaccggttc cggtaaaacg gaggtttatc
    10441 tcagtgtact ggaaaacgtg ctcgctcagg gcaaacaggc gctggtgatg gtgccggaaa
    10501 tcggcctgac gccgcaaact atcgcccgtt ttcgtgaacg ttttaatgcg ccagtggaag
    10561 ttctgcattc cggcctgaac gacagcgagc gtctttcggc gtggttgaaa gcgaaaaatg
    10621 gtgaggcggc gattgtgatc ggcacccgct ccgcgctgtt tacgccgttt aaaaatctcg
    10681 gtgtgattgt cattgatgaa gagcacgaca gttcctacaa gcagcaggaa ggctggcgct
    10741 atcatgcccg cgacctggcg gtttatcgtg cgcacagcga gcaaatcccg attattcttg
    10801 gctccgcaac acccgcactg gaaacgttat gcaacgtcca gcagaaaaaa taccgcctgc
    10861 tgcgcctgac gcgtcgggcg gggaatgcgc gtccggcaat tcaacacgtg ctggatttaa
    10921 aaggtcagaa ggtgcaggcg gggctggctc cggcgttaat cacccgtatg cgccagcatt
    10981 tacaggccga caaccaggtt atcctctttc ttaaccgccg tggctttgcg cctgccttgc
    11041 tgtgccacga ctgcggctgg attgccgaat gtccacgttg cgatcactac tacacgctgc
    11101 atcaggcgca acagcatttg cgctgtcacc actgtgacag ccaacgcccg gtgccgcgcc
    11161 agtgcccttc ctgcggttcc acgcacctgg tgccggtggg gctgggcacc gaacagcttg
    11221 aacagacgct tgcgccgctg ttccctggtg tgcccatttc tcgtatcgac cgcgatacca
    11281 ccagccgcaa aggagcgctg gaacaacaac tggcggaagt gcatcgcggc ggcgcgagga
    11341 tcttgatcgg cacgcaaatg ctggcgaaag gtcaccactt tccggatgtg acgctggtgg
    11401 cgttactgga cgtagacggc gcgctgtttt ctgccgattt tcgctcggca gagcgtttcg
    11461 cccagcttta cacccaggtc gccggtcgtg ccgggcgtgc gggtaaacag ggcgaagtgg
    11521 tgctgcaaac gcatcatcca gaacacccgc tattgcaaac gttgctctat aaaggctatg
    11581 acgcctttgc cgaacaggca ctggctgagc gacgcatgat gcagctgccg ccgtggacca
    11641 gccatgtgat tgtgcgtgcg gaagatcata acaatcagca cgcgccgctg ttcctgcaac
    11701 aactgcgtaa tctcatcctc tccagcccgc tggcagacga caaactgtgg gttctcggtc
    11761 ccgttccggc actggcacct aaacgtggcg gccgctggcg ctggcaaatt ttgttgcagc
    11821 acccttcccg cgtgcgatta cagcacatca tcaatggtac gctggcgctt attaatacaa
    11881 taccggattc ccgtaaggtg aaatgggtgc tggatgtcga tcccatcgag ggctaatgcg
    11941 ctcacgatgc gaggcggatc gaaaaattca atattcatca cacttttcat gaaaattctg
    12001 taaccgtttt cacgcgctat ctgctaaaaa tgttgccgat gtgaagtaaa catggatgta
    12061 gtacgcctga cgtgccaggc gaggagtgag tgtgaaagcg aagaagcagg aaactgccgc
    12121 gaccatgaaa gacgttgccc tcaaggcaaa agtctctaca gcgactgtct cccgagcatt
    12181 aatgaatccc gataaagtct cccaggccac ccgtaatcgg gttgaaaaag cggcccggga
    12241 agtgggttat ttaccgcagc ctatggggcg caacgtcaag cgtaatgaat cccgcactat
    12301 tctggtgatt gtcccggata tctgcgatcc cttctttagc gaaattattc gcggtatcga
    12361 agttacggcg gcaaatcacg gatatctggt gctgattggc gactgtgcgc atcaaaatca
    12421 gcaggaaaaa acctttatcg atttgatcat caccaagcaa attgatggca tgttgctgct
    12481 gggttcaagg ctgccgtttg atgccagcat tgaggaacag cgtaatctgc cgccgatggt
    12541 gatggcgaac gaatttgcac cggaactgga gctgcctacc gttcatatcg acaatctgac
    12601 cgccgcattt gatgcagtaa attatttata tgagcaaggg cataatcgga ttggctgtat
    12661 agccggtccc gaagagatgc cgctgtgtca ctaccgcctg caaggctatg tccaggcgct
    12721 gcgtcgctgc ggcattatgg ttgatccgca atacatcgcc cgtggcgact tcaccttcga
    12781 agccggaagc aaagcgatgc agcagttgct tgatcttcca caaccgccta ctgctgtctt
    12841 ctgccatagc gatgtgatgg cgctcggcgc actttctcag gcaaaacgcc aggggctgaa
    12901 agtcccggaa gacctttcca taatcggttt tgataacatc gacctgacgc aattttgtga
    12961 tccgccgctg acaaccatcg cgcagccgcg ttacgaaatc ggtcgggaag ctatgctgtt
    13021 attgcttgat caaatgcagg ggcaacacgt tggcagtggc tctcgtttaa tggactgcga
    13081 acttatcatc cggggatcaa cacgcgcgtt accttaaagt aaacctttaa gactgccgtg
    13141 actggtcaaa ggcccgccgc ttaagtaaca tggcgggctg acgaacgaat aaatacagcg
    13201 aaacgatagt ggcacaacga gattatgtac gccgcagcca accggcacct tcgcggcgaa
    13261 aaaagagcac ctcacggaaa aagcaacgaa atctgcctgc ggtttctccc gctatggtcg
    13321 ctattgctgc cgccgttctt gtgaccttta tcggtggtct gtacttcatt acgcatcaca
    13381 agaaagaaga gtccgagaca ctgcaaagcc agaaagtgac cggaaacgga ctaccaccaa
    13441 aaccagaaga acgctggcgc tacatcaaag agctggaaag tcgccagccg ggtatgcgtg
    13501 cgcctacaga accttctgcc ggtggtgaag tgaaaacgcc ggagcaactg acaccagaac
    13561 aacgccagct tcttgagcaa atgcaggctg atatgcgcca gcagccaacg cagctggttg
    13621 aagttccgtg gaatgaacag acgccggaac agcgtcagca aacgctacag cgccagcgtc
    13681 aggcgcagca attagcggag cagcaacgcc tggctcagca gtcgcgtacg actgaacaaa
    13741 gctggcagca gcagacgcgt acgtcgcaag ccgcgccggt acaggcgcaa ccgcgccagt
    13801 ctaaaccggc ttctactcag cagccgtacc aggatctgct gcaaacccct gcgcacacga
    13861 ccgcgcagtc gaagccgcag caagctgcgc cggttgctcg tgccgctgac gcgccaaaac
    13921 cgacggcgga gaaaaaagac gaacgccgct ggatggtgca gtgcggttcg ttcagaggcg
    13981 cggaacaggc agagacggta cgtgctcagc tggcgttcga aggctttgac tcaaaaatta
    14041 ccaccaacaa tggctggaat cgtgtggtga ttggcccggt gaaaggcaaa gagaacgcag
    14101 acagcaccct caatcgcttg aagatagcgg gtcatacaaa ctgcattcgg ctcgccgccg
    14161 ggggttgaaa ccctcaaaat cccccccatc tataattgca ttatgccccg tacttttgta
    14221 cggggtttgt actctgtatt cgtaaccaag gggtcagctc gtgacaacta tagtaagcgt
    14281 acgccgtaac ggccatgtgg tcatcgctgg tgatggtcag gccacgttgg gcaataccgt
    14341 aatgaaaggc aacgtgaaaa aggtccgccg tctgtacaac gacaaagtca tcgcgggctt
    14401 tgcgggcggt actgcggatg cttttacgct gttcgaactg tttgaacgta aactggaaat
    14461 gcatcagggc catctggtca aagccgccgt tgagctggcg aaagactggc gtaccgatcg
    14521 catgctgcgc aaacttgaag cattgctggc agtcgcggat gaaactgcat cgcttatcat
    14581 caccggtaac ggtgacgtgg tgcagccaga aaacgatctt attgctatcg gctccggcgg
    14641 cccttacgcc caggctgcgg cgcgcgcgct gttagaaaac actgaactta gcgcccgtga
    14701 aattgctgaa aaggcgttgg atattgcagg cgacatttgc atctatacca accatttcca
    14761 caccatcgaa gaattaagct acaaagcgta aggatctccc atgtctgaaa tgaccccacg
    14821 cgaaatcgtc agcgaactgg ataagcacat catcggccag gacaacgcca agcgttccgt
    14881 ggcgattgct ctgcgtaacc gctggcgtcg catgcagctc aacgaagagc tgcgccatga
    14941 agtgaccccg aaaaatatcc tgatgatcgg cccgaccggt gtcggtaaaa ctgaaatcgc
    15001 ccgtcgcctg gctaagctgg cgaatgcgcc gttcatcaaa gttgaagcga ccaaattcac
    15061 cgaagtgggc tatgtcggta aggaagtgga ttctattatt cgcgatctga ccgatgccgc
    15121 cgtgaaaatg gtacgcgtcc aggctatcga gaaaaaccgt tatcgcgctg aagaactggc
    15181 agaagaacgt attctcgacg tactgatccc accggctaaa aataactggg gacagaccga
    15241 acagcagcag gaaccgtccg ctgctcgtca ggcattccgc aaaaaactgc gtgaaggcca
    15301 gcttgatgac aaagaaatcg agatcgatct tgccgcagca ccgatgggcg ttgaaattat
    15361 ggctcctccg ggcatggaag agatgaccag ccagctgcag tccatgttcc agaacctggg
    15421 cggtcagaaa caaaaagcgc gtaagctgaa aatcaaagac gccatgaagc tgctgattga
    15481 agaagaagcg gcgaaactgg tgaacccgga agagctgaag caagatgcta tcgacgctgt
    15541 tgagcagcac gggatcgtgt ttatcgacga aatcgacaaa atctgtaagc gcggcgagtc
    15601 ttccggtccg gatgtttctc gtgaaggcgt tcagcgtgac ctgctgccgc tggtagaagg
    15661 ttgcactgtt tccaccaaac atgggatggt caaaactgac cacattctgt ttatcgcttc
    15721 tggcgcgttc cagattgcga aaccgtctga cctgatcccg gaactgcaag gtcgtctgcc
    15781 aatccgcgtt gagctgcagg cactgaccac cagcgacttc gagcgtattc tgaccgagcc
    15841 gaatgcctct atcaccgtgc agtacaaagc actgatggca actgaaggcg taaatatcga
    15901 gtttaccgac tccggtatta agcgcatcgc ggaagcggca tggcaggtga acgaatctac
    15961 cgaaaacatc ggtgctcgtc gtttacatac cgttctggag cgtttaatgg aagagatttc
    16021 ctacgacgcc agcgatttaa gcggtcaaac tatcactatt gacgcagatt atgtgagcaa
    16081 acatctggat gcgttggtgg cagatgaaga tctgagccgt tttatcctat aatcgcgttc
    16141 aatcattttc atcattgttt gatggggctg aaaggcccca tttttattgg cgcgtattat
    16201 gactgaacaa caaattagcc gaactcaggc gtggctggaa agtttacgac ctaaaaccct
    16261 ccccctcgcc tttgctgcaa ttatcgtcgg gacggcgctg gcatggtggc aaggtcactt
    16321 cgatccactg gtcgccctgc tggcattgat taccgccggg ctattacaga tcctttctaa
    16381 cctcgccaat gattacggcg atgcggtaaa aggcagcgat aaacctgacc gcattgggcc
    16441 gctacgcggc atgcaaaaag gggtcattac ccagcaagag atgaaacggg cgctcattat
    16501 taccgttgtg ctcatctgtc tttccgggct ggcactggtt gcagtggcgt gccatacgct
    16561 ggccgatttt gtcggtttcc tgattcttgg cgggttgtcg atcattgccg ctatcaccta
    16621 caccgtgggc aatcgtcctt atggttatat cggtttgggt gatatttccg tactggtttt
    16681 ctttggctgg ttgagcgtca tggggagctg gtatttacag gctcatacat tgattccggc
    16741 actgatcctt ccggcaaccg catgcggcct gctggcaacg gcagtactga acattaataa
    16801 cctgcgtgat atcaatagcg accgcgaaaa cggcaaaaac acgctggtgg tgcgcttagg
    16861 tgcagtaaac gcgcgtcgtt atcatgcctg cctgctgatg ggctcactgg tgtgtctggc
    16921 gctgtttaat ctcttttcgc tgcatagcct gtggggctgg ctgttcctgc tggcggcacc
    16981 attactggtg aagcaagccc gttatgtgat gcgggaaatg gacccggtgg cgatgcgacc
    17041 gatgctggaa cgcactgtca agggagcgtt actgactaac ctgctgtttg ttttagggat
    17101 attcctaagc cagtgggcag cataactgac aaatatcaat taacaattga tgattttgcc
    17161 aacagcctac atagcgcgat atactgaaaa ttctcgcagc aactgaatgt taagcctatg
    17221 aaatacgata cttccgagct ttgtgacatc tatcaagaag atgttaacgt cgtggaaccg
    17281 ctgttctcca actttggcgg acgggcgtcg tttggcggac aaataatcac ggtaaaatgt
    17341 ttcgaggaca acgggttgct gtacgatctg ctcgaacaga atggccgtgg tcgtgttctt
    17401 gtcgtcgatg gcggtggttc tgttcgtcgc gcactggtcg atgctgaact ggcgcgtctg
    17461 gcagtacaaa atgaatggga aggtctggtc atttacggcg cggtgcgtca ggtagatgac
    17521 ctggaagagc tggatatcgg catccaggca atggccgcaa ttccggttgg tgccgctggc
    17581 gaaggcattg gcgaaagcga tgtccgcgtc aattttggcg gtgtaacctt cttctccggc
    17641 gaccatcttt acgccgacaa taccgggatt attctttcag aagatccgct ggatattgaa
    17701 tgataaaaaa ggcaccgcaa ggtgcctttt ttctgcgtta cctgttggcc tacacagtaa
    17761 agaaattacg cgaaagatga agcgtaatca gacctcttcc atgcgaccca gcagggcctg
    17821 cagacgttcc tgccagccgt tctgctgttc tttcagatgg ttgttctcac gctccagctc
    17881 ttcgcgctga tgctgggcat tttgaacttc ctgcgacagt gagttgtttt tttctttcag
    17941 ctcttcgatt tccatctgca acagagtgat ggtatcaatc gcctgctgta cttttgcttc
    18001 cagtttctca aacacttcta atgacattgt catacctctc ctgaattgca aggcgttgat
    18061 ggataaaaaa tcctcgtccc gattaccggt gacgccttaa taaatacgag cgcactttag
    18121 ttagctccga ttgtatgaag ccgcgccatc gctgtccagc ggcacgcctt gcagattacg
    18181 gtttgccaca cttttcatcc ttctcctgga gacataatcc acaccaatcg aaaatgttaa
    18241 taaatttgtt gcgcgaatga tctaacaaac atgcatcatg tacaatcaga tggaataaat
    18301 ggcgcgataa cgctcatttt atgacgaagc acacacattt taagttcgat atttctcgtt
    18361 tttgctcgtt aacgataagt ttacagcatg cctacaagca tcgtggaggt ccgtgacttt
    18421 cacgcataca acaaacatta actcttcagg atccgattat gagtcaaaca tcaaccttga
    18481 aaggccagtg cattgctgaa ttcctcggta ccgggttgtt gatttttttc ggtgtgggtt
    18541 gcgttgcagc actaaaagtc gctggtgcgt cttttggtca gtgggaaatc agtgtcattt
    18601 ggggactggg ggtggcaatg gccatctacc tgaccgcagg ggtttccggc gcgcatctta
    18661 accccgctgt taccattgca ttgtggctgt ttgcctgttt cgacaagcgc aaagttatcc
    18721 cttttatcgt ttcacaagtt gccggcgctt tctgtgctgc ggctttagtt tacgggcttt
    18781 actacaattt atttttcgac ttcgagcaga ctcatcatat tgttcgcggc agtgttgaaa
    18841 gtgttgatct ggctggcact ttctctactt accctaatcc tcatatcaat tttgtgcagg
    18901 ctttcgcagt tgagatggtg attaccgcta ttctgatggg gctgatcctg gcgttaacgg
    18961 acgatggcaa cggtgtacca cgcggccctt tggctccctt gctgattggt ctactgattg
    19021 cggtcattgg cgcatctatg ggcccattga cgggttttgc catgaaccca gcgcgtgact
    19081 tcggtccgaa agtctttgcc tggctggcgg gctggggcaa tgtcgccttt accggcggca
    19141 gagacattcc ttacttcctg gtgccgcttt ttggccctat cgttggcgcg attgtaggtg
    19201 catttgccta ccgcaaactg attggtcgcc atttgccttg cgatatctgt gttgtggaag
    19261 aaaaggagac cacaactcct tcagaacaaa aagcttcgct gtaatatgac tacgggacaa
    19321 ttaaacatga ctgaaaaaaa atatatcgtt gcgctcgacc agggcaccac cagctcccgc
    19381 gcggtcgtaa tggatcacga tgccaatatc attagcgtgt cgcagcgcga atttgagcaa
    19441 atctacccaa aaccaggctg ggtagaacac gacccaatgg aaatctgggc cacccaaagc
    19501 tccacgctgg tagaagtgct ggcgaaagcc gatatcagtt ccgatcaaat tgcagctatc
    19561 ggtattacga accagcgtga aaccactatt gtctgggaaa aagaaaccgg caagcctatc
    19621 tataacgcca ttgtctggca gtgccgtcgt accgcagaaa tctgcgagca tttaaaacgt
    19681 gacggtttag aagattatat ccgcagcaat accggtctgg tgattgaccc gtacttctct
    19741 ggcaccaaag tgaagtggat cctcgaccat gtggaaggct ctcgcgagcg tgcgcgtcgt
    19801 ggtgaattgc tgtttggtac ggttgatacg tggcttatct ggaaaatgac tcagggccgt
    19861 gtccatgtga ccgattacac caacgcctct cgtaccatgt tgttcaacat ccataccctg
    19921 gactgggacg acaaaatgct ggaagtgctg gatattccgc gcgagatgct gccagaagtg
    19981 cgtcgttctt ccgaagtgta cggtcagact aacattggcg gcaaaggcgg cacgcgtatt
    20041 ccaatctccg ggatcgccgg tgaccagcag gcggcgctgt ttggtcagtt gtgcgtgaaa
    20101 gaaggaatgg cgaagaacac ctatggcact ggctgcttta tgctgatgaa cactggcgag
    20161 aaagcggtga aatcagaaaa cggcctgctg accaccatcg cctgcggccc gactggcgaa
    20221 gtaaactatg cgctggaagg tgcggtgttt atggcgggcg cgtccattca gtggctgcgc
    20281 gatgagatga agttgattaa cgacgcctac gattccgaat atttcgccac caaagtgcaa
    20341 aacaccaatg gtgtgtatgt ggttccggcc tttaccgggc tgggtgcgcc atactgggat
    20401 ccgtatgcac gcggggcgat tttcggtctg actcgtgggg tgaacgctaa ccacattata
    20461 cgcgcgacgc tggagtctat tgcttatcag acgcgtgacg tgctggaagc aatgcaggcc
    20521 gactccggta ttcgtctgca cgcgctgcgc gtggacggcg gtgcggtggc gaacaatttc
    20581 ctgatgcagt tccagtccga tattctcggt acccgcgttg agcgcccgga agtgcgcgaa
    20641 gtcaccgctt tgggtgcggc ctatcttgct ggtctggcgg ttggcttctg gcagaatctt
    20701 gacgagctgc aagagaaagc ggtgattgaa cgcgagttcc gtccaggcat cgaaaccact
    20761 gagcgtaatt accgttacgc aggctggaaa aaagcggtca aacgcgcgat ggcatgggaa
    20821 gaacacgacg agtaataatg attgcctgat gcgacgcttg cgcatcttat caggcctacg
    20881 agtcggatac acatccggca tcacgcagac tctgaaaccg tgcgggggca accccgcaca
    20941 catcaataat cccgcccttc ccctgtgcta cacttcgcgc cattccttac tgcttagagt
    21001 ttgctatgag acgagaactt gccatcgaat tttcccgcgt caccgaatca gcggcgctgg
    21061 ctggctacaa atggttagga cgcggcgata aaaacaccgc ggacggcgcg gcggtaaacg
    21121 ccatgcgtat tatgctcaac caggtcaaca ttgacggcac catcgtcatt ggtgaaggtg
    21181 aaatcgacga agcaccgatg ctctacattg gtgaaaaagt cggtactggt cgcggcgacg
    21241 cggtagatat tgctgttgat ccgattgaag gcacgcgcat gacggcgatg ggccaggcta
    21301 acgcgctggc ggtgctggca gtaggcgata aaggctgttt cctcaacgcg ccggatatgt
    21361 atatggagaa gctgatcgtc ggaccgggag ccaaaggcac cattgatctg aacctgccgc
    21421 tggcggataa cctgcgcaat gttgcggcgg cgctcggcaa accgttgagc gaactgacgg
    21481 taacgattct ggcaaaaccg cgccacgatg ccgttatcgc agaaatgcag caactcggcg
    21541 tgcgcgtatt tgctattccg gacggcgacg ttgcagcctc aattctcacc tgtatgccag
    21601 acagcgaagt tgacgtgctg tacggtattg gtggcgcgcc ggaaggcgtg gtttctgcgg
    21661 cggtgatccg cgcattagat ggtgacatga acggtcgtct gctggcgcgt catgacgtca
    21721 aaggcgacaa cgaagagaat cgtcgcattg gcgagcagga gctggcacgc tgcaaagcga
    21781 tgggcatcga agccggtaaa gtattgcgtt tgggcgatat ggcgcgcagc gacaacgtca
    21841 tcttttctgc caccggcatc accaaaggtg atctgctgga aggtattagc cgcaaaggca
    21901 atatcgccac caccgaaacg ctgctgatcc gtggtaaatc acgcaccatt cgtcgcattc
    21961 agtccatcca ctatctggat cgtaaagacc cggaaatgca ggtgcacatc ctctgattga
    22021 tttgatcgat tgagccttcc agtccttcgg gactggaatt tttttgttcg gagaacgaag
    22081 ataaggcaag tcaatcaaaa caggagaaaa acatggctga ttgggtaaca ggcaaagtca
    22141 ctaaagtgca gaactggacc gacgccctgt ttagtctcac cgttcacgct cccgtgcttc
    22201 cgtttaccgc cgggcaattc accaaacttg gccttgaaat tgaaggcgaa cgcgttcagc
    22261 gcgcctactc ctatgttaac tcgcccgata atccggatct ggagttttac ctggtcaccg
    22321 tccccgatgg caaattaagt ccacgactgg cggcactgaa accgggcgat gaagtgcagg
    22381 tggttagcga agccgctggc ttctttgtgc tggatgaagt accagattgc gaaacgctat
    22441 ggatgctggc aaccggtaca gcgattggcc cttatttatc gattctgcaa ttgggcaaag
    22501 atttagaacg cttcaaaaac atggtgctgg ttcacgccgc acgatatgcc gccgacttaa
    22561 gctatctacc tctgatgcag gaactggaaa aacgctacga ggggaaactg cgcattcaga
    22621 cggtggtcag tcgggaaacg gctgcgggtt cgctcactgg gcgattaccg gcgttaattg
    22681 aaagtggtga actggaaagc gcggttggcc tgccgatgaa taaagaaacc agccatgtga
    22741 tgctgtgcgg caatccacag atggtgcgcg atacacaaca gttgctgaag gagacccggc
    22801 agatgacgaa acatttacgt cgccgaccgg gccatatgac agcggagcat tactggtaag
    22861 cggttacttg tcgataaacg gcacgatcaa gagatccgca ctcaacttat tgatcatccc
    22921 gcgatatgcc ggcatcaaac ggttgataaa tgagtgatga tgaccacaga caaggaggtc
    22981 gcactgctct ttttgcataa tttccagcag cgtttccggc atttctccgc gttcaatacg
    23041 cagttttgtc ttcggccatt gaatattttt cgtcagttta tacagcttgt tatccgactt
    23101 attcttcaac aattgaagaa tatcttctgt tgcagggaag tagatacccg gatacaactc
    23161 gcttaagcca tcatcaatat gaattaacgt caggtgagcg tcattatgtc tggcgagctc
    23221 cagggcttta ttcaccagta aggcatcttc ttcattcccg gaaattgcca cgccaatgtg
    23281 tttataagcc atgtttaact ccttctaaag caactccatc aagctaacga acgcagtgat
    23341 aactcaaaaa taatcatctc ggcctggcat gagaaggtga aggccgcatc aagctcaact
    23401 tgcccctcca gatccttaat cttataggcc acgctggccc cttcatctgc agaggatgca
    23461 gcggcggcaa gctctttcag tttggtcata aaagactctg cttcttcgcg ggtggcaaaa
    23521 aagcgcgaaa acttactggt acagttgtcg ttatcaatca ccgtaccgat atcaatcgca
    23581 catcctttag tactgcattt atctacgaca tctttcatag ggcacctctg tatattcgcc
    23641 tctctgtcga ttacgactta agaataatgc gccgttaaag gaagaacaaa caagtaatga
    23701 tcttatggat taccattttt tttgatattg cttaaggtga agagttcaga aggattttcc
    23761 gggcattgtc acttgcatcg tggaaataac aatcccggaa aaaaatagcg ataagagata
    23821 tattaaaggt taattacttg atttattgtc ggctttatac ttcacatcct gggtatcttt
    23881 accatattta ttttcgcctt gtgtgccaac aaacgcacca agatcgataa gcatcatcac
    23941 cagaatcaat gtcgggacaa aacgccccac cgcccattgc cagacacccg gtaaaattgc
    24001 ccagttaccc gccagcagca tccacgccac aatcatcaga aatgcccatg cgccggaacg
    24061 cccgcgatca tgcaagcgct taacggttac tgccgctgtt ggccaaagca agcacacaag
    24121 gcaaaacgcc gcggtctgaa tatcgagtaa atttttaccc gccagtgaga aaagcaccag
    24181 catgcctgcg aaccacaggc ctatccaaat ccagaaatca cggcgtccaa tacgcccttt
    24241 aaatgagaat aaccattgct gtatggtcat gtaagttcct tgatggttgt cttttccagg
    24301 attctaccct tttgacaagg gcgacagata tcgttttaat cggagccagt cataacaaaa
    24361 ggtactgtca atgaagccag ggtgtacgct gttttttctc ttatgttctg cattaaccgt
    24421 tacaacaacg gcgcatgcac aaacaccaga tacggcaacg accgcgcctt atctgctggc
    24481 tggagcccct actttcgatc tctccatcag ccagtttcga gaagacttta acagccagaa
    24541 tcccagcctg ccactgaacg aatttcgtgc catcgacagc agtcccgaca aagccaatct
    24601 cactcgtgca gcaagtaaaa ttaacgagaa cttgtatgct tctactgcgc tggagcgcgg
    24661 taccttaaaa atcaaaagca ttcaaatgac ctggctaccc atccaggggc cagagcaaaa
    24721 agccgcgaaa gcgaaagctc aggcatacat ggcagcggtg atccgcacac tcaccccatt
    24781 aatgaccaaa actcaaagcc agaaaaaact gcaggcgcta ctaacggcgg ggaaaaacaa
    24841 acgttattac accgagacag aaggtgcact gcgttatgtt gtcgcggaca acggcgaaaa
    24901 ggggctgacc ttcgctgttg aaccgattaa gctggcgcta tctgaatcgc ttgaaggttt
    24961 gaataaatga caaaaagcaa agcctttgtg ccgatgaatc tctatactgt ttcacagacc
    25021 tgctgccctg cggggcggcc atcttccttt attcgcttat aagcgtggag aattaaaatg
    25081 cgacatcctt tagtgatggg taactggaaa ctgaacggca gccgccacat ggttcacgag
    25141 ctggtatcta acctgcgtaa agagctggca ggtgttgctg gctgtgcggt tgcaatcgca
    25201 ccaccggaaa tgtacatcga tatggcgaag cgtgaagctg aaggcagcca catcatgctg
    25261 ggtgcgcaaa acgtggacct gaacctgtcc ggcgcattca ccggtgaaac ctctgctgct
    25321 atgctgaaag acatcggcgc acagtacatc atcatcggcc actctgaacg tcgtacttac
    25381 cacaaagagt ctgacgaact gatcgcgaaa aaattcgcgg tgctgaaaga gcagggtctg
    25441 actccggttc tgtgcatcgg tgaaaccgaa gctgaaaacg aagcgggcaa aactgaagaa
    25501 gtttgtgcac gtcagatcga cgcggtactg aaaactcagg gtgctgcggc attcgaaggt
    25561 gcggttatcg cttacgaacc cgtatgggca atcggtactg gcaaatctgc aactccggct
    25621 caggcacagg ctgttcacaa attcatccgt gaccacatcg ctaaagttga cgctaacatc
    25681 gctgaacaag tgatcattca gtacggcggc tctgtaaacg cgtctaacgc tgcagaactg
    25741 tttgctcaac cagacatcga cggcgcgctg gttggcggtg cttctctgaa agctgacgct
    25801 ttcgcagtga tcgttaaagc tgcagaagcg gctaaacagg cttaagtctg acaggtgccg
    25861 gatttcatat ccggcactta ctttccttaa ctcctcgcct taacgcaaaa tctcacactg
    25921 atgatcctgg atttcctcgg ctgaagcacg gttaagcgtc agtagatttc gttgtgtcgc
    25981 cagcaataca aatgagttat cactctgccg caccatcgcc agcccgtagc ttcccatatg
    26041 ttcccgcgcc tcaggcactt cttctgcaag catcataaat gggctgcgtt gtaccagttc
    26101 gctttccgtt acccgacgcg ccaggtattc atgcccgcgc aaaccacctg gcagtggcaa
    26161 ccagcggctg ctgatgttcg ccagattgtt atccagctgt tcgcgcacat caggacgaat
    26221 acaagagata tgaatatgaa aatggttttg cgtacgcccg gtgcgagagt tgatcgccaa
    26281 agaaaccgcg cgatcgggaa ccggctggcc gtattttttg ctcataaaat cacgcgcctg
    26341 ccaggccaac caaaagaagt tcggcgttga aggatcggtc aacaaaggac tttcagtacc
    26401 gttaatacga tacgttggca tcaacagata ttgcagtggg ccattaagat cttttaaaac
    26461 cacgtatccg gcattgggtt tgacttccgc acatggcgaa ggattttgat tttgctgctg
    26521 attgggcaaa cattcctcaa ggacaatctt acgtaatgta tccgactctt caccggttaa
    26581 tttccagtaa ccaataccgg cagccacaac ggcgataact atcatcacca aaaaaagaag
    26641 acccgctttt ttcatctttt ttccctgtac ctcaaagaga tgcaagggta acgcaaaatc
    26701 gtgacaaata aaaaacccag tcgcgagaaa ccaccgggtt tgataattat cctggagaaa
    26761 tcagcgtttg ctaatctgat cgaacgtacc gccgttagca aaatgctctt tttgcgcttt
    26821 cgtccagccg ccgaactctt catcaatggt gaataacttc agctttggaa acgcattttc
    26881 gtactttttc gccacctcag catcgcgcgg acggtagtag tttttcgcgg caatttcctg
    26941 accttctggc gagtagagat atttcaggta ggcttccgcc acctctttgg tgcctttttt
    27001 ctcgaccact ttatcgacca ccgacacggt tggctctgcg aggatagact cactcggcgt
    27061 gacaatttcg aatttatctt tccccagttc attcgctgcc agcagagctt cgttttccca
    27121 ggcaatcagt acatcgccaa tcccgcgctc gacaaaggta ttagtggagc cgcgcgcgcc
    27181 agaatccaga acttcgacgt ttttatacag tgcccgaaca aaatcctgtg cttttgcctg
    27241 atcgttgttg ttgtgatgca gcgcgtagcc ccaggctgcc aggtagttcc agcgcgcgcc
    27301 accagagctt ttcggattag gcgtgatcac cgaaacaccc ggtttaatca gatcgttcca
    27361 gtcatggatc tgcttcggat tgcccttacg caccaggaaa acaatggtgg aagtgtacgg
    27421 tgcggagtta tccggcagac gtttgatcca ctctttatca atccgcccgc gttccgcaat
    27481 cgcgtccacg tcataggcca gagccagcgt gacaacatca gcttcaatac cgttgattac
    27541 cgacgtcgct tgtttgcctg aaccaccgtg ggactgacgg atcaccacgt tatcgccagt
    27601 ttgctgtttc cagtgggcgc tgaatacctt gttgtactgt tcgtacaatt cgcgcgttgg
    27661 atcatatgaa acgttaagaa gctgaatatc ctttgccata acgctggttg ccgccagcaa
    27721 aaatgttaac cctacgcccc acttgttcat cgcccgactc tcttatgttg tgttgtgatg
    27781 agcaaagcgt gccagaaggt taaccaaaca ttaaagaata aaaaaagatt ggctataact
    27841 tgcgggtata tgtaggggga ttaaaaaggc ggaagaaatc cgcctcatat tgctgacaaa
    27901 gtgcgctttg tccatgccgg atgcggcgcg aacgccttat ccggcctaca aaagttcgca
    27961 aattcaataa attgcagaat tcatgtaggc ctgataagcg aagcgcatca ggcatttttg
    28021 cttctgtcat cggtttcagg ctaaaggaat ctgccttttt ccgaaatcat taatacagtt
    28081 ttttcgcgca gtccagccag tcgcctttga acggacgctt catgttttcg atagcgtcga
    28141 tgatgtcgtg gtgaaccagc tgttcgttct ggatacctac gcaacgacca ccgtaacctg
    28201 ccagcagcag ctcgatagcg taagcgccca tacgggaagc cagaatacgg tcgtaaggca
    28261 ccggagaacc accgcgctgg atgtggccca gcacagttgc gcgggtttca cgaccggttt
    28321 ctttctcgat gaaatgcgcc agttcgtcaa catcacacat atgttcggta atcgccacta
    28381 tcgcgtgttt tttacctttc gcgatacccg ctttgatttc gtttaccagg tcttcacggc
    28441 tgaattcaac ttccggaacc acaacgaatt cacagccacc ggcaatagcc gcagccaaag
    28501 tcagatcgcc acaataacgg cccatcactt ccaccacgga aatacgctgg tgagaagaag
    28561 aggtgtcacg cagacggtcg atcgcttcta caacggtgct cagcgcagtg aagaaaccga
    28621 tagtgtagtc agtgcctttg atgtcgttgt cgatagtgcc cggcagaccg atgcacggga
    28681 agcccatttc ggtcagacgc attgcaccca tgtaggaacc gtcaccgccg ataaccacca
    28741 gcgcgtcgat accacgtttt ttcaggtttt cgatagccac ggcgcggatg ttctcgtcgc
    28801 ggaattccgg gaaacgcgca gaaccgagga acgtaccacc acggttgatc atgtcagaaa
    28861 cgctgtaacg gtctagctgt accatacggt cttcatacag acccagatag ccgtcataaa
    28921 tgcccattac ttccagacct tctgtcagcg cagaacgaac aaccccgcga attgcggcgt
    28981 tcatgcctgg cgcatcaccg ccgcttgtca acacaccgat tttcttaatc atgactacct
    29041 ctgaactttg gaatgcaaaa tgaaatctgt tgccggaagt cttcttgcac atcgaagtga
    29101 tccaacgaat gtgcaaatag tataacaatc acttcctgct gaattgattc aggtcaggcc
    29161 aaatggcggt attttataca caaaatgcgg gtctggctct cttttatact gattatgaaa
    29221 gcatagaccg tttaccctcc ctgggtacga cggaacaggg gtcctgatgg ataatcacat
    29281 ccgatcccgg aaaacgccgt aaaatagcct gctctacctg atccgccacc atatgtgcct
    29341 gaaccaaagg cagagagtct tccatttcca aatgaatctg aataaagcgg gtcggccctg
    29401 actgccgcgt gcgaagatcg tgagcgccgc taacacccgg ccaggaagtc acgatatcaa
    29461 taatttcttg ccgttcttca tcaggcaatg cgcgatccag taatgactgt accgcctcat
    29521 atcccatgcg taacgcgcta tataaaatat agatgccgat tcccaatgca aacagagcat
    29581 cggcgcgatg ccagccgtac caggacaacc ccagcgccag cagaattgca ccgttcatca
    29641 taacatcaga ctggtaatgt agcatatcag cccgcaccgc ctggctttgc gtccggcgca
    29701 ccacccaacg ctgaaacgag acaaggataa tcgtacaaat tagcgccaca attgtcacga
    29761 taaccccgac gcctggatct gtcatcggtg ttggagatat cagatgttga atacccgtca
    29821 aaaacaggaa tagtgctgaa ccggagataa acatactttg cgccagcgcc gcgagggact
    29881 cagctttacc gtgaccaaac gagtgattat cgtcggcagg ttgcagggaa tatcgcacca
    29941 ccagtaaatt cgtcaacgac gcgccgatat ccaccagcga atccaccagc gcggcgagaa
    30001 tactcaccga cccggtatac caccatgcaa aaattttaat cagcaatagc agtgaagcca
    30061 tcgccgttgc agcaatcgcc gcccgactga ccagccgtcc ataagattga ttcataaata
    30121 ctcccgctat caactgacgc tagtataacg gaagcaaatc atctgcaatg cattaagcag
    30181 caggcaaatt gaggataaaa aaaaccccca catcatgtgg gggaagacag ggatggtgtc
    30241 tatggcaagg aaaacagggt ttactactgg gaacgtgagt tgctactact caatagcttc
    30301 aacgatgaac ttttttgcca ttgcgtcacg tcacgcaact gctccattcg ttgttgatgt
    30361 ttctcattta aaaccgcttg ctgctccggc gttaacaggc gatacatttg gttgcggact
    30421 tttgccatct caacctgacg agcaatttgc tcattcgcca ttttttctgc ctgtgcgcgc
    30481 acagcgtttt catcaaaatt ttctgcggtg acaaggcgat gcattgtctc cagttcgcta
    30541 acattaacag gaggctgttc gtgccgggcc tgttgcataa gatctcgcat ctgctgacgc
    30601 tgatgttcgg ttaaacttat gccgtcgaac atatggctct gcgtactgcg ctgcgtaagt
    30661 tcttcacccg gatgccagtt atcgcctgaa ccgacttcag cagcgtggct taatgaactg
    30721 actgccagcg ttgaggccat gacggcagcg gtaactatgc gcatcatttg ctcccaaaat
    30781 ctttctgtcg cgattcaacg atagagagtt tacgattcag gctgcaaaca tgcgtcaggg
    30841 ggtgtaaaac aacgtaaagt catggattag cgacgtctga tgacgtaatt tctgcctcgg
    30901 aggtatttaa acaatgaata aaatcctgtt agttgatgat gaccgagagc tgacttccct
    30961 attaaaggag ctgctcgaga tggaaggctt caacgtgatt gttgcccacg atggggaaca
    31021 ggcgcttgat cttctggacg acagcattga tttacttttg cttgatgtaa tgatgccgaa
    31081 gaaaaatggt atcgacacat taaaagcact tcgccagaca caccagacgc ctgtcattat
    31141 gttgacggcg cgcggcagtg aacttgatcg cgttctcggc cttgagctgg gcgcagatga
    31201 ctatctcccg aaaccgttta atgatcgtga gctggtggca cgtattcgcg cgatcctgcg
    31261 ccgttcgcac tggagcgaac aacagcaaaa caacgacaac ggttcaccga cactggaagt
    31321 tgatgcctta agtctcaatc ctggtcgcca ggaagccacc ttcgacgggc aaacactgga
    31381 gttaaccggt actgagttta ccctgctcta tttgctggca cagcatctgg gtcaggtggt
    31441 ttcccgtgag catttaagcc aggaagtgct gggcaagcgc ctgacgcctt tcgaccgcgc
    31501 tatcgatatg catatttcca acctgcgtcg taaactgccg gatcgtaagg atggtcaccc
    31561 gtggtttaaa accctgcgtg gtcgtggcta tctgatggtt tctgcttcat gataggcagc
    31621 ttaaccgcgc gcatcttcgc catcttctgg ctgacgctgg cgctggtgtt gatgttggtt
    31681 ttgatgttac ccaagctcga ttcacgccag atgaccgagc ttctggatag cgaacagcgt
    31741 cagggtctga tgattgagca gcatgttgaa gcggagctgg cgaacgatcc gcccaacgat
    31801 ttaatgtggt ggcggcgtct gttccgggcg attgataagt gggcaccgcc aggacagcgt
    31861 ttgttattgg tgaccaccga aggccgcgtg atcggcgctg aacgcagcga aatgcagatc
    31921 attcgtaact ttattggtca ggccgataac gccgatcatc cgcagaagaa aaagtatggc
    31981 cgcgtggaac tggtcggtcc gttctccgtg cgtgatggcg aagataatta ccaactttat
    32041 ctgattcgtc cggccagcag ttctcaatcc gatttcataa acttactgtt tgaccgcccg
    32101 ctattactgc tgattgtcac catgctggtc agtacgccgc tgctgttgtg gttggcctgg
    32161 agtctggcaa aaccggcgcg taagctgaaa aacgctgccg atgaagttgc ccaaggaaac
    32221 ttacgccagc atccggaact ggaagcgggg ccacaggaat tccttgccgc cggtgccagt
    32281 tttaaccaga tggtcaccgc gctggagcgc atgatgacct ctcagcaacg tctactttct
    32341 gatatctctc acgaactgcg caccccgctg acgcgtctgc aactgggtac ggcgttactg
    32401 cgccgtcgta gcggtgaaag caaggaactg gagcgtattg aaaccgaagc gcaacgtctg
    32461 gacagcatga tcaacgatct gttggtgatg tcacgtaatc agcagaaaaa tgcgctggtt
    32521 agcgagacca tcaaagccaa ccagttgtgg agtgaagtgc tggataacgc ggcgttcgaa
    32581 gccgagcaga tgggcaagtc gttgacggtt aacttcccgc ctgggccgtg gccgctgtac
    32641 ggcaacccga acgccctgga gagtgcgctg gaaaacattg ttcgtaatgc cctgcgttat
    32701 tcccatacga agattgaagt gggctttgcg gtagataaag acggtatcac cattacggtg
    32761 gacgacgatg gtcctggcgt tagcccggaa gatcgcgaac agattttccg tccgttctat
    32821 cgtaccgatg aagcacgcga tcgtgaatct ggcggtacag gtttggggct ggcgattgta
    32881 gaaaccgcca ttcagcagca tcgtggctgg gtgaaggcag aagacagccc gctgggcggt
    32941 ttacggctgg tgatttggtt gccgctgtat aagcggagtt aaactccgca tttgtaggca
    33001 ggataaggcg tttacgccgc atccggcatt tgagcaggat gcctgatgcg acgctgatag
    33061 cgtcttatca ggcctacact ccggggatta ttttccccac aatcggcggg aaaaatcttc
    33121 gatcttgccg ctcagtcggc gcttttgcat cgttcgcgtc cagcttttcg atacccctgc
    33181 ggcggagagt aaacggtgat actgatcgtc atcaaacggc atatgccatg cgatggcggc
    33241 agcctcctgc acggtcacat cgctgacacg ggaaaccaac tccagtggcg catcagcaga
    33301 taccaaccct ggtgcaatta cgctatacaa ccaccccact ttacccgtgt tttgcatcaa
    33361 ctgcgctata tcgctgatat caaaatggta attgagttta tagcaaggtg agcgtggctg
    33421 gctgacctga attaatgcct ctccccagcg gaaaatatcg ccaatataaa cattactttc
    33481 cgtcagaccg tcggttgaga gattttcgcc aaacgcaggc gcaacaaaca actccgcctg
    33541 ttccggaaat tcccgcgccc agtaaagata atgctcacga ggataatgac acagcgctct
    33601 gtctggcccg ccgtgaacct ttttctccgc ctgctcgtca ccttccagcc ccaggtccgt
    33661 cagcatcaac tcaccatcaa cctggatttt ggcaattgcg ctgggtttgc cttcgggata
    33721 agcctgaatc ttgcctgtgt atacatcaac cggatatcgc atctgttccc tctccacgag
    33781 aaatttcccc attagaccat atttatttta tgtcaggcgt gctgttttcg cggtaagtat
    33841 ccaggcagga actcaatagc aaaccggacg aacgttagtt catccggctg tggggattaa
    33901 ggaagcaggt ttatttcacc gtacctaaaa tttcgatttt cgctgctctg gctttgactt
    33961 tacgtgacca gatagaggta agaattggca ccagaatcga agtcacaatg accgccgtcg
    34021 cgaccagcga agttgctgcc ggagccatcg gtttaaacgc aggcaccatt tctgcaatca
    34081 gcacaggtgt cgctaccgcg gcccctgcgg aactggaagc ggcaattccg gcagtgccat
    34141 cgccaccgcc aatcaattta tcagcgataa tcaacggaat accggtcacg ataattactg
    34201 ccacacccaa caggatcccc agcaaacctg tctgggcaat taccgtcaaa tcaatggtat
    34261 tacccagtgc gaaggcaaag aatggaatca gcgtctgcac cgctttgctg aaaaattctc
    34321 gtaattcagg gtcaaggttc ccaagggcaa agcccaccag gaacggtaat actgcgccga
    34381 cgaaaacatg cggttcaaac gaggcaatcc cggcagtgcc cagaataatc atcgtcatga
    34441 gcggcccgga ctccaatgac atcaacacaa atgccccagc ttcttctttt gtgccgtact
    34501 gctgcatgat ggaagcgtaa agtccgccgt tggtcatatc catcgccgcc accagcgcca
    34561 gcgttgaaag tccggcaaag aatccaactt caacaccatg ttccggaata atgcgcgagg
    34621 caattgccgc aaccacccac gcgacggcaa tttttgttac caccagcgta ccggatttac
    34681 gcaataccgt tcccgtcgcg cttaatttta ttgacgcccc catgcaaaaa aaccacaccg
    34741 ccagaatggg caccgtaccg gtaatcatcc cgttggtgaa tgatccaaaa tatttccccg
    34801 cctccggcga gaaggtgtgg cacagtgcgc caaggaataa cgggacgagc atcatccccc
    34861 ccgggatttt ctcaatcgag cgttttatct gcatttccat cacctgtcgg catattttta
    34921 agggctgatg cgtcattcct caatggagca tcagtgttga tggaaacgaa gatagacatt
    34981 catttaatca ataaaagtga tcaaaaccac atcaattgaa acactgtttt atttttatcg
    35041 gatcattgtt tttattttga aattgatcac aaaaaaacca ccgccgttgg cgatggttca
    35101 ttttttaagc tgatatgcgg cgtaaacgcc tcattgcagc aggcggcaaa tgattatttt
    35161 ttcgccgcga aacgtgccgc tgcttcgtcc cagttcacca cgttccagaa ctctttgatg
    35221 tagtcaggac ggcggttctg gaatttcagg tagtaagcat gttcccacac atccaggccc
    35281 agaatcggga agccggaagc gccagaaata gcttcaccca tcagcgggga atcctggtta
    35341 gcagtagaaa ccaccgccag tttatcgcct ttcagcacca gccatgccca gccggaacca
    35401 aagcgggaag ctgccgcttt ctcaaattct gctttgaagt tatcaacgga gccgaagtca
    35461 cgttcgatag ccgctttcag gtcaccctgc agggtggtgc cttttttcag acctttccag
    35521 aacaggctgt ggttagcgtg accgccagcg ttgttgcgca gtacggtttt cttgtctgct
    35581 ggcagctggt ccagtttggt gatcagctct tcaaccggca ggttggcaaa ttctggcagg
    35641 ctttccagcg ccgcgttggc gttgttgacg taggtctgat ggtgtttggt gtggtggatt
    35701 tccatggtct gcttatcgaa gtgcggttcc agggcatcgt aagcatacgg cagggatggc
    35761 agggtataac tcatattcat ctccagtgtt gtcgggcggc cgattgttaa tgccgcgtaa
    35821 gcagttggtt cattatagtt aattaaatga tattgaaaat gattatcaat gccgtacttt
    35881 tcgtaagggt atggttttgc aggaaaatgc ccgagatgtg aagcaaatca cccacttaat
    35941 gccgtgattg ccagtaaatc gacaacggcg gcaacaggcg aaaggttaat cgacagcacg
    36001 atttttacac tcatctcgtc ggagatgtga cgcgacgaaa aatgatgagg ataagaagat
    36061 gagtaacgcg attacgatgg ggatattttg gcatttgatc ggcgcggcca gtgcagcctg
    36121 tttttacgct ccgttcaaaa aagtaaaaaa atggtcatgg gaaaccatgt ggtcagttgg
    36181 tgggattgtt tcgtggatta ttctgccgtg ggccatcagc gccctgttac taccgaattt
    36241 ctgggcgtat tacagctcgt ttagtctctc tacgctgctg cctgtttttc tgttcggcgc
    36301 aatgtggggg attggtaata tcaactacgg cctgaccatg cgttatctcg gcatgtcgat
    36361 ggggattggt atcgccattg gtatcacgtt gattgttggt acgctgatga cgccaattat
    36421 caacggcaat ttcgatgtgt tgatcaatac cgaaggtgga cgcatgacat tgctcggcgt
    36481 tctggtggcg ctgattggtg tagggattgt gactcgcgcc gggcagttga aagagcgcaa
    36541 gatgggcatt aaagccgaag agttcaacct gaaaaaaggg ctggtgctgg cggtgatgtg
    36601 cggcattttc tctgccggaa tgtcctttgc gatgaacgcc gcaaaaccga tgcatgaagc
    36661 cgctgccgca ctgggcgtcg atccactgta tgtcgctctg ccaagctatg ttgtcatcat
    36721 gggcggcggc gcgatcatta acctcggttt ctgcttcatt cgtctggcaa aagtgaagga
    36781 tttgtcgcta aaagccgact tctcgctggc aaaaccgctg atcattcaca atgtgttact
    36841 ctcagcactg ggcgggttga tgtggtatct gcaattcttt ttctatgcct ggggccacgc
    36901 ccgcattccg gcgcagtatg actacatcag ttggatgctg catatgagct tctatgtatt
    36961 gtgcggcggt atcgtcgggt tggtgctgaa agagtggaac aatgcaggtc gccgtccggt
    37021 aacggtgttg agcctcggtt gtgtggtgat tattgtcgcc gccaacatcg tcggcatggg
    37081 catggcgaat taatctttct gcgaattgag atgacgccac tggctgggcg tcatcccggt
    37141 ttcccgggta aacaccaccg aaaaatagtt actatcttca aagccacatt cggttgaaat
    37201 atcactgatt aacaggcggc tatgctggag aagatattgc gcatgacaca ctctgacctg
    37261 ccgcagatat tgattgatgg tcattccagt ctgctggcga aattgctgac gcaaaacgcg
    37321 ctcactgcac gatgcctcat cacaaaattt atccagcgca aagggacttt tcaggctggc
    37381 cgccagccgg gtaatcagct tatccagcaa cgtttcgctg gatgttggcg gcaacgaatc
    37441 actggtgtaa cgatggcgat tcagcaacat caccaactgc ccgaacagca actctgccat
    37501 ttcgttagca aacggcacat gctgactact ttcatgctca agctgaccga taatctgccg
    37561 cgcctgcgcc atccccacgc tacctaagcg ccagtgtggt tgccctgcgc tggcactaaa
    37621 tcccggaatc gccccctgcc agtcaagatt cagcttcagc cgctccgggc aataaataat
    37681 attctgcaaa accagatcgt taacggaagc gtaggagtgt ttatcgtcag catgaatgta
    37741 aaagagatcg ccacgggtaa tgcgataagg gcgatcgttg agtacatgca gaccattacc
    37801 gcgccagaca attaccagct cacaaaaatc atgtgtatgt tcagcaaaga catcttgcgg
    37861 ataacggtca gccacagcga ctgcctgctg gtcgctggca aaaaaatcat ctttgagaag
    37921 ttttaactga tgcgccaccg tggctacctc ggccagagaa cgaagttgat tattcgcaat
    37981 atggcgtaca aatacgttga gaagattcgc gttattgcag aaaaccatcc cgtccctgac
    38041 gaatatcacg cggtgaccag ttaaactctc ggcgaaaaag cgtcgaaaag tggttactgt
    38101 cgctgaatcc gcagcgatag gcgatgtcag taacgctggc ctcgctgtgg cgtagcagat
    38161 atcgggcttt catcagtcgc aggcggttca ggtaacgctg aggcgtcagc cccgtttgct
    38221 gcttaagctg gcgatgcagc gtacgcaatg aaagagaaaa ttgatccgcc acggcatccc
    38281 aattcacctc atcggcaaaa tggtcctcca gccaggccag aagcaagttg agacgtgacg
    38341 cgctgttttc caggttctcc tgcaaactgc ttttacgcag caagagcagt aattgcataa
    38401 ataagatctc gcgactggcg gtcgagggta aatcattttc ctcttcctgc tgttccatct
    38461 gtgcaaccag ctgtcgcacc tgctgcaaca cgctgtggtt aacgcgccag tgagacggat
    38521 actgcccatc ctgctcttgt ggcagcaact gattcagccc ggcgagaaac tgaaatcgat
    38581 ccggcgagcg atacagcaca ttggtcagac acagattatc ggtatgttca tacagatgcc
    38641 gatcatgatc gcgtacgaaa cagaccgtgc caccggtgat ggtatagggc tgcccattaa
    38701 acacatgaat acccgtgcca tgttcgacaa tcacaatttc atgaaaatca tgatgatgtt
    38761 caggaaaatc cgcctgcggg agccggggtt ctatcgccac ggacgcgtta ccagacggaa
    38821 aaaaatccac actatgtaat acggtcatac tggcctcctg atgtcgtcaa cacggcgaaa
    38881 tagtaatcac gaggtcaggt tcttacctta aattttcgac gggaaaccac gtaaaaaacg
    38941 tcgatttttc aagatacagc gtgaattttc aggaaatgcg gtgagcatca caccaccaca
    39001 attcagcgaa ttgtgaacat catcacgttc atctttccct ggttgccaat gacccatttt
    39061 cctgtcagta acgagaaggt cgcgaattca ggcgcttttt agactggtcg taatgaaatt
    39121 cagcaggatc acattatgac ctttcgcaat tgtgtcgccg tcgatctcgg cgcatccagt
    39181 ggacgcgtga tgctggcgcg ttacgagcgt gaatgccgca gcctgacgct gcgcgaaatc
    39241 catcgtttta acaatgggct gcatagccag aacggttatg tcacctggga tgtggatagc
    39301 ctggaaagtg ccattcgcct tggattaaac aaggtgtgcg aggaagggat tcgtatcgat
    39361 agcattggga ttgatacctg gggcgtggac tttgtgctgc ttgaccaaca gggtcagcgc
    39421 gtgggcctgc ctgttgctta tcgcgatagc cgcacccatg gtctgatggc gcaggcacaa
    39481 cggcaactcg gcaaatgcga tatttatcaa cgtagcggca tccagttttt gcccttcaat
    39541 acgctttatc agttgcgtgc gctgacggag caacaacctg aacttattcc acacattgct
    39601 cacgctctgc tgatgccgga ttacttcagt tatcgtctga ccggcaagat gaactgggaa
    39661 tataccaacg ccacgaccac gcaactggtc aatatcaata gcgacgactg ggacgagtcg
    39721 ctactggcgt ggagcggggc caacaaagcc tggtttggtc gcccgacgca tccgggtaat
    39781 gtcattgggc actggatttg cccgcagagt aatgagattc cggtggtcgc cgttgccagc
    39841 catgataccg ccagcgcggt tatcgcctcg ccgttaaaca gttcacgcgc cgcttatctc
    39901 tcttctggca cctggtcatt gatgggcttc gaaagccaga cgccatttac caatgacacg
    39961 gcgctggcag ccaacatcac caatgaaggc ggggcggaag gtcgctatcg ggtgctgaaa
    40021 aatattatgg gcttatggct gcttcagcgg gtgcttcagg agcggcaaat caacgatctt
    40081 ccggcgctta tcgccgcgac acaggcactt ccggcttgcc gcttcaccat caatcccaat
    40141 gacgatcgct ttattaatcc tgacgagatg tgcagcgaaa ttcaggctgc gtgtcgggaa
    40201 acggcgcaac caatcccgga aagtgatgct gaactggcgc gctgtatttt cgacagtctg
    40261 gcgctgctgt atgccgatgt gttgcatgag ctggcgcaac tgcgcggtga agatttctca
    40321 caactgcata ttgtcggcgg tggctgccag aacgcgctgc tcaaccagtt atgtgctgat
    40381 gcctgcggta ttcgggtgat cgccgggcct gttgaagcct cgacgctcgg caatatcggc
    40441 atccagttaa tgacgctgga tgaactcaac aatgtggatg atttccgtca ggtcgtcagc
    40501 accaccgcga atctgaccac ctttacccct aatcctgaca gtgaaattgc ccactatgtg
    40561 gcgcagattc actctacacg acagacaaag gagctttgcg catgaccact caactggaac
    40621 aggcctggga gctggcgaaa cagcgtttcg cggcagtagg gattgatgtc gaggaggcgc
    40681 tgcgccaact tgatcgttta cccgtttcaa tgcactgctg gcagggcgat gatgtttccg
    40741 gttttgaaaa cccggaaggt tcgctgaccg gtgggattca ggctactggt aattatccgg
    40801 gcaaagcgcg taatgccagc gagctacgtg ccgatctgga acaagctatg cggctgattc
    40861 cggggccgaa acggcttaat ttacatgcca tctatctgga atcagatacg ccagtcgcgc
    40921 gcgaccaaat caaaccagag cacttcaaaa actgggttga atgggcgaaa accaatcagc
    40981 tcggtctgga ttttaacccc tcctgttttt cgcatccgct aagcgccgat ggctttacgc
    41041 tttcccatgc cgacgacagt attcgccagt tctggattga tcactgcaag gccagccgcc
    41101 gtgtttcagc ctattttggc gagcaactcg gcacaccgtc ggtgatgaac atctggatcc
    41161 cggatggcat gaaagatatc accgttgacc gtctcgcccc gcgccagcgt ctgctggcag
    41221 cactggatga ggtgatcagc gagaagctgg atccggcgca tcatatcgat gccgttgaga
    41281 gcaaattgtt tggcattggc gcggagagct acacggttgg ctccaatgag ttttacctgg
    41341 ggtatgccac cagccgccag acggcgctgt gcctggacgc cgggcatttc cacccgactg
    41401 aagtgatttc cgacaagatt tccgccgcca tgctgtatgt gccgcagttg ctgctgcacg
    41461 tcagccgtcc ggttcgctgg gacagcgatc acgtagtgct gctggatgat gaaacccagg
    41521 cgattgccag tgagattgtt cgtcacgatc tgttcgaccg ggtgcatatc ggcctcgact
    41581 tcttcgatgc ctctatcaac cgcattgccg cgtgggtcat tggtacacgc aatatgaaaa
    41641 aagccctgct acgtgcgtta ctggaaccta ccgctgaact acgcaagctg gaatcggcgg
    41701 gcgattacac tgcgcgtctg gcgctactgg aagagcagaa atcgttgccg tggcaggcgg
    41761 tatgggaaat gtattgtcag cgtcacgata cgccagcggg tagcgaatgg ctggagaacg
    41821 tgcgggctta tgagaaagaa attttgagtc gccgcgggta aacactgccg gatgcggcac
    41881 aagtgcctta tccggcctac gggtcggcaa tagttgtagg cctgataaga cgcgacagcg
    41941 tcgcatcagg cattgattgc cggatgcggc acgagtgcct tatccggcct acagatcgta
    42001 tatccggcaa caggcacgga cgtccaatta acaggaacac cgaacatgca aaacattact
    42061 cagtcctggt ttgtccaggg aatgattaaa gccaccaccg acgcctggct gaaaggctgg
    42121 gacgagcgca acggcggcaa cctgacgctg cgcctggatg acgccgatat cgcaccatat
    42181 cacgacaatt ttcaccaaca accgcgctat atcccgctca gccagcccat gcctttactg
    42241 gcaaatacac cgtttattgt caccggctca ggcaaattct tccgtaacgt ccagcttgat
    42301 cctgcggcta acttaggcat cgtaaaagtc gacagcgacg gcgcgggcta ccacattctt
    42361 tgggggttaa ccaacaaagc cgtccccact tccgaacttc cggctcactt tctctcccac
    42421 tgcgagcgca ttaaagccac caacggtaaa gatcgggtga tcatgcactg ccatgccacc
    42481 aacctgatcg cccttaccta cgtactggaa aacgacaccg cggtcttcac tcgccaactg
    42541 tgggaaggca gcaccgaatg tctggtagtg ttcccggatg gcgtcggcat tttgccgtgg
    42601 atggtgcccg gcaccgacga aatcggccag gcgaccgcac aggaaatgca aaaacattcg
    42661 ctggtgttgt ggcccttcca cggcgtcttc ggcagcggtc cgacactgga tgaaacattc
    42721 ggtttaatcg acaccgcaga aaaatcagca caagtattag tgaaggttta ttcgatgggc
    42781 ggcatgaaac agaccatcag tcgtgaagag ctgatagcgc tcggccagcg tttcggcgtt
    42841 acaccactcg ccagtgcgct ggcgctgtaa ggagcaaaca tgatccgcaa agcctttgtc
    42901 atgcaggtta accccgacgc ccacgaagag tatcagcgtc gtcataatcc aatctggccg
    42961 gaactggaag cagtgttgaa atctcacggt gcgcataact acgccattta cctcgacaaa
    43021 gcgcgtaatc tgctgtttgc cacggtagag attgaatctg aagaacggtg gaatgcggta
    43081 gccagtactg atgtttgcca acgttggtgg aaatatatga ccgacgttat gccaactaac
    43141 ccggataaca gcccagtcag tagcgagctg caagaagtgt tttacctgcc ttaatgagtt
    43201 gcggtgccgg aaaatatcca gcaccgcgac ttcatcagaa gaagtattta aagcgaacgc
    43261 gtgcaccgta acggtcatca gtatcatcac cgtgtttttt atcaccgtcg ctatccagaa
    43321 tggagtaata cgtcccgagg taaatattaa agttctccat atccatcaca ttagcgaact
    43381 gataagacgc atgaatggtg tgaatggtgt acgtcccttc atcatcaatc cagcagtcgt
    43441 tatcacaaac tacaccgctg aattcatcaa tcttattgtg cgcgtagata taacccagct
    43501 cgaaacgttt ccacagcgcg ttaatcccgg cagtgaaatc tttctcatta ttagcatcta
    43561 aataggcggt attaagatta accacgacac cattttccgg atccgttttc aggccattcc
    43621 aggtcatgct cataccatag ccggtacgat cggactgatc gacaaaatta cccttgctat
    43681 cggtataacc ataagcatta tttaccacgt tcgcttccat cgctgcggaa acggtgaatt
    43741 cttccgtcgg cgaccaggca attaccggac gcagataagc aacatttttc tgctgttcca
    43801 tatcgcgtcc atgataatta ccgtcgttat ataaagatgt tccgtcttcc agtaacgtgt
    43861 ttaattcaaa ataccagtta tcgagttgtt tgctgacgag gaaattaccg ccagcgttag
    43921 aacgcccgcg gccctctttc atcatataga tataaccgct gccatcgtca taaagatcgt
    43981 tcgcagtatt accggaatgt tcaacaaagg tatcctgatt cagcgggaac atatcgtagg
    44041 cttcaaaacg gcctactttg accttccagt cgttttcttt gccaaagaag aataccgcat
    44101 catcgatatt tactgaaccg tgcatgtccc ccagcggttg tgcggagaac ccggcgaaat
    44161 aaccgttgtc catctttcgc ataccgtcaa aacctaacag aatacgacca ttgagattcc
    44221 actgttcatt agtgggatcg ctattcacat cagcgttggt cattgccaga aggccatgat
    44281 tactttccgc atccatatta aattctacgt cgccgtagat ttttaaatcg ccataacctg
    44341 aaagcaaaag attaggtgag gtggaatttt tctttgcaga ttccgacgtt gcctgtccat
    44401 ttaccggcgt attgccctgg gcagccttaa tttcgcggat ctcagcggct tgttgtgtct
    44461 gcagctgttt aacctggctc tctgcttttg ctgcccgcat ttctgccgct tccaggcgtt
    44521 cttccagcaa tgccatgcgt tgttccagcg ttaaatgctg tgtttttgcc attccggtac
    44581 tacatgccaa taataaaccg atggctaaag ataatgtcga ttttttcatt atttacgttc
    44641 cctgaaataa aagagcccct acgctttcca aaaggaatac gcatatagag aaatataagg
    44701 tgtgcacttt cgaattttta atccaacttc agcaggccga tggatagtat cccgccatat
    44761 aatatgagta aatccgctga tttcctggaa cgtgaggaat ataacaatag tgccgttttt
    44821 aactaaatat cgtaaaaaat attagatatg attcacgctg actcataagc cacaaatata
    44881 aataacgctt aaacaaatta tctgagacat ttaaacattc acaaaatcat taaataatga
    44941 tactttattc acaaaattta tccttcactc tgctttatat tgtcacgact tcgtgacata
    45001 tccgcgctca tcacaaaccc gctccgcgat tacaattgac tgaaatattt catcgaccat
    45061 ttcgttgtac tcgcataatg ctctcgggaa atgagtcttc ggccatccag cttgagtttg
    45121 cgtcctgaag ttgctcttta tcccagtcag agtacgggac tttatctctg atgcggcgaa
    45181 cgtatttttt agcctcgagt tgatactggc ctgccgaatt aataaacgag aaattcatgc
    45241 cgcttttatc cgcttctttg gcctgcgcca ccagcgtttc cagttcagac actttcttca
    45301 ttgccgcttc tgcatcaaac ttatcttgcg atatcaggtt attaatttgt ttcgctgaaa
    45361 tcatgactgc cagagagtag tagtggaatg ttttcccttc tctttcttca atatttttca
    45421 attccgtaag ctggcgcttg tcattaattt cctgaatcgc cgcatgataa gattctgcta
    45481 caggttcaaa ggcttccaga ttttttaaga atgtctggtg cagcgttttc cctttcgcaa
    45541 acgcatcgtc tttataattt tcctggatgt aatatttatc catttcgtta atggtattac
    45601 ccaacgctac ggcagcatca atataactca ccgccacgcc atcaataggt tgcaacg
//
LOCUS       DAZGQL010000030        45033 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.030, whole genome shotgun sequence.
ACCESSION   DAZGQL010000030 DAZGQL010000000
VERSION     DAZGQL010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 45033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45033
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.030"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            126..241
                     /gene="rrf"
                     /locus_tag="ACHKIR_001922"
     rRNA            126..241
                     /gene="rrf"
                     /locus_tag="ACHKIR_001922"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            567..1865
                     /gene="kgtP"
                     /locus_tag="ACHKIR_001923"
     CDS             567..1865
                     /gene="kgtP"
                     /locus_tag="ACHKIR_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HFO9989670.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLTLHRKGKGMRL"
     gene            complement(1862..2185)
                     /gene="yfiM"
                     /locus_tag="ACHKIR_001924"
     CDS             complement(1862..2185)
                     /gene="yfiM"
                     /locus_tag="ACHKIR_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HFO9989671.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YAQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDFAWDIAGASTGYTVW
                     QLTRH"
     gene            complement(2229..3584)
                     /gene="pssA"
                     /locus_tag="ACHKIR_001925"
     CDS             complement(2229..3584)
                     /gene="pssA"
                     /locus_tag="ACHKIR_001925"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HFO9989672.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3698..6358)
                     /gene="pat"
                     /locus_tag="ACHKIR_001926"
     CDS             complement(3698..6358)
                     /gene="pat"
                     /locus_tag="ACHKIR_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HFO9989673.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKSNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AISNPLDLRDDASSEHYIKTLDILLHSQDFDALMVIHSPSAAAPATESAQALIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RIHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDIAPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            complement(6390..7088)
                     /gene="tapT"
                     /locus_tag="ACHKIR_001927"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6390..7088)
                     /gene="tapT"
                     /locus_tag="ACHKIR_001927"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HFO9989674.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPETLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSLDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAEGQYCTAEVAIALLDMAGDTEAATGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(7157..7576)
                     /gene="trxC"
                     /locus_tag="ACHKIR_001928"
     CDS             complement(7157..7576)
                     /gene="trxC"
                     /locus_tag="ACHKIR_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HFO9989675.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7783..8820
                     /gene="yfiF"
                     /locus_tag="ACHKIR_001929"
     CDS             7783..8820
                     /gene="yfiF"
                     /locus_tag="ACHKIR_001929"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFO9989676.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEESPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVLQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8868..9557)
                     /gene="ung"
                     /locus_tag="ACHKIR_001930"
     CDS             complement(8868..9557)
                     /gene="ung"
                     /locus_tag="ACHKIR_001930"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFO9989677.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIATPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREDVV
                     FLLWGSHAQKKGAIIDKQRHHILKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGEKPI
                     DWMPVLPAESE"
     gene            9862..10245
                     /gene="grcA"
                     /locus_tag="ACHKIR_001931"
     CDS             9862..10245
                     /gene="grcA"
                     /locus_tag="ACHKIR_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HFO9989678.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGFAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10301..10888)
                     /gene="eamB"
                     /locus_tag="ACHKIR_001932"
     CDS             complement(10301..10888)
                     /gene="eamB"
                     /locus_tag="ACHKIR_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HFO9989679.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATTHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQT
                     KPISFWASFALQFVNVKIILYGITALSTFVLPQTQALSWIIGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10991..11872
                     /gene="yfiE"
                     /locus_tag="ACHKIR_001933"
     CDS             10991..11872
                     /gene="yfiE"
                     /locus_tag="ACHKIR_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO9989680.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVISGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQPLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPRFTVANELASGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEECFLPG"
     gene            complement(11905..13239)
                     /gene="srmB"
                     /locus_tag="ACHKIR_001934"
     CDS             complement(11905..13239)
                     /gene="srmB"
                     /locus_tag="ACHKIR_001934"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HFO9989681.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13371..14108
                     /gene="trmN"
                     /locus_tag="ACHKIR_001935"
     CDS             13371..14108
                     /gene="trmN"
                     /locus_tag="ACHKIR_001935"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HFO9989682.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTDDSVMIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(14093..15715)
                     /gene="nadB"
                     /locus_tag="ACHKIR_001936"
     CDS             complement(14093..15715)
                     /gene="nadB"
                     /locus_tag="ACHKIR_001936"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HFO9989683.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHIQPNGAESYHLTREGGHSHRRILHAADATGREVETTLVSKALNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDDRGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDITRCMPYAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSPGNHYINR"
     gene            15971..16126
                     /gene="rseD"
                     /locus_tag="ACHKIR_001937"
     CDS             15971..16126
                     /gene="rseD"
                     /locus_tag="ACHKIR_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HFO9989684.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            16123..16698
                     /gene="rpoE"
                     /locus_tag="ACHKIR_001938"
     CDS             16123..16698
                     /gene="rpoE"
                     /locus_tag="ACHKIR_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HFO9989685.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            16731..17381
                     /gene="rseA"
                     /locus_tag="ACHKIR_001939"
     CDS             16731..17381
                     /gene="rseA"
                     /locus_tag="ACHKIR_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HFO9989686.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            17381..18337
                     /gene="rseB"
                     /locus_tag="ACHKIR_001940"
     CDS             17381..18337
                     /gene="rseB"
                     /locus_tag="ACHKIR_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HFO9989687.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            18334..18813
                     /gene="rseC"
                     /locus_tag="ACHKIR_001941"
     CDS             18334..18813
                     /gene="rseC"
                     /locus_tag="ACHKIR_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HFO9989688.1"
                     /translation="MIKEWATVVSWENGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DIAALCGAILGGVGGFLIARGCSRKFAARSEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            19011..20810
                     /gene="lepA"
                     /locus_tag="ACHKIR_001942"
     CDS             19011..20810
                     /gene="lepA"
                     /locus_tag="ACHKIR_001942"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFO9989689.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            20826..21800
                     /gene="lepB"
                     /locus_tag="ACHKIR_001943"
     CDS             20826..21800
                     /gene="lepB"
                     /locus_tag="ACHKIR_001943"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFO9989690.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            22072..22752
                     /gene="rnc"
                     /locus_tag="ACHKIR_001944"
     CDS             22072..22752
                     /gene="rnc"
                     /locus_tag="ACHKIR_001944"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFO9989691.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            22749..23654
                     /gene="era"
                     /locus_tag="ACHKIR_001945"
     CDS             22749..23654
                     /gene="era"
                     /locus_tag="ACHKIR_001945"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFO9989692.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLRDGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            23666..24394
                     /gene="recO"
                     /locus_tag="ACHKIR_001946"
     CDS             23666..24394
                     /gene="recO"
                     /locus_tag="ACHKIR_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFO9989693.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTHCAGSGESVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            24406..25137
                     /gene="pdxJ"
                     /locus_tag="ACHKIR_001947"
     CDS             24406..25137
                     /gene="pdxJ"
                     /locus_tag="ACHKIR_001947"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HFO9989694.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQRDKMRDACKRLAEAGIPVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELARIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            25137..25517
                     /gene="acpS"
                     /locus_tag="ACHKIR_001948"
     CDS             25137..25517
                     /gene="acpS"
                     /locus_tag="ACHKIR_001948"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFO9989695.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            25939..26019
                     /gene="shoB"
                     /locus_tag="ACHKIR_001949"
     CDS             25939..26019
                     /gene="shoB"
                     /locus_tag="ACHKIR_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HFO9989696.1"
                     /translation="MTDCRYLIKRIIKIIIAVLQLILLFL"
     gene            complement(26213..26473)
                     /gene="yfhL"
                     /locus_tag="ACHKIR_001950"
     CDS             complement(26213..26473)
                     /gene="yfhL"
                     /locus_tag="ACHKIR_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HFO9989697.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPQHVETEEQLWDKFVLMHHADKI"
     gene            26688..28085
                     /locus_tag="ACHKIR_001951"
     CDS             26688..28085
                     /locus_tag="ACHKIR_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO9989698.1"
                     /translation="MPRTVTHNPDSPNNDDVLAASEKWDACKPPYTSAHMKICVAAAK
                     IILAASGVARRSKYEKENYLRIDFSKAGKVTFYAEFPKKMGLKGKKLGEWPELAIQLA
                     REKALGMADCGLRAESVHAALEMYRDDLKAKVARQKLSPDSFTTYGVRIDRIKATFGE
                     REVFSDVTYNRLVEVLDEWIATRSNNNALELFAELRRFWKFCAPTLCNGRNVAASLPD
                     DYVSSRVQKPTPTRLFTDIESIARLWLNVAACTSVHQKNAVRFMIITGVRPINVHNLR
                     WDYVHEEAGEIVYPEGVIGMRGAMKTQKAFRLPITPEIRRIIDEQKAWRDSVPECNRD
                     YVFLQPRDPMQPFSKRSLDKLVKTYSPDGAVKGIKHDGTVKGKDGAFNTMCRKFLKSN
                     VIALMKERGYSRSDRREISLLCLHHSSKSDDPMAEHYDFSDEILQEEIALKREAFEAH
                     ERSILAQVALLRRRG"
     gene            complement(28279..29673)
                     /locus_tag="ACHKIR_001952"
     CDS             complement(28279..29673)
                     /locus_tag="ACHKIR_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HFO9989699.1"
                     /translation="MGQIFTPRPYQDLIINHQIETLRGNIWAGMGMGKTVATLTALED
                     LFMAGAETRPALVLAPLRVAASTWPDEALKWGHLRNIEVQPIVGNAKARSAALANSNA
                     SVFTINYDNLVWLVEELGERWPFGTVIPDESTRLKSFRLRGGGKRAAALGKVAHKYVQ
                     RWINLTGTPAPNGLVDLWGQLWFVDQGQRLGRTYGAFTSRWFNSIQFPGQSWTKLEPF
                     AHSQAEIQRALADVTLSLDAADWFDIKDPIHNVIRVDMPPKARQQYREMEKEMFLELN
                     GEGIEAPNAAAKTLKCLQIASGAVYTDDTGSWSELHDTKLQALDSILTEAAGAPVLVA
                     YHWKHDLERLLKAFPRGRHLDQDPQTLRDWNSGKIPVLFAHPASAGHGLNMQDGGNIL
                     VFFSHWWDLEQYQQIIERIGPTRQIQAGHNRPVFIHHIIAADTMDEMVMERRNSKRTV
                     QDILLDAMKKRGIA"
     gene            complement(29936..30451)
                     /locus_tag="ACHKIR_001953"
     CDS             complement(29936..30451)
                     /locus_tag="ACHKIR_001953"
                     /inference="COORDINATES: protein motif:HMM:NF021206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFO9989700.1"
                     /translation="MFETTLTLAGTGKQPMMSSREIAELTSKEHKIVVRDIKALLEQL
                     GFHSTEMYHHENKDFLVKRKIYNGREVIDEILLDQDLSTTLVTGYSAHDRYKVVKRWR
                     ELETGKVQPLVTHPSTPKIQINDDIVRLARVVAEATMKACLDATGYSQPAAPANLSHV
                     EGEYVPVSKAA"
     gene            complement(30512..30688)
                     /locus_tag="ACHKIR_001954"
     CDS             complement(30512..30688)
                     /locus_tag="ACHKIR_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9989701.1"
                     /translation="MAIQITQLHQADIFGYIADMLETARLLSSLEKGEQLAFELIYFA
                     QQAAREAANKPWDE"
     gene            30801..31130
                     /locus_tag="ACHKIR_001955"
     CDS             30801..31130
                     /locus_tag="ACHKIR_001955"
                     /inference="COORDINATES: protein motif:HMM:NF015807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HFO9989702.1"
                     /translation="MSKRDDPQLRVRIPELLKEALEKKARANKRTLTSEIVSRLESTI
                     LQDKLLHTSRGFEEAADEILILRDLLEKLKTTYHREYQVEWVYENKYELIEVMEKLKK
                     LLNPENE"
     gene            complement(31099..31275)
                     /locus_tag="ACHKIR_001956"
     CDS             complement(31099..31275)
                     /locus_tag="ACHKIR_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HFO9989703.1"
                     /translation="MSKRDDPQLRVRIPQDLKDTLEKISRENDRTLTAEITRRLRKSL
                     EDESTIHSQDLATS"
     gene            complement(31272..31541)
                     /locus_tag="ACHKIR_001957"
     CDS             complement(31272..31541)
                     /locus_tag="ACHKIR_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HFO9989704.1"
                     /translation="MAYERESLIEKHLVAEVKKAGGVAFKFVSPGRRSVPDRIVLLPG
                     GRIVFVECKAPGKPPRPDQLREHERLRKLGFTVVVLDSKNLEGIL"
     gene            complement(31541..32023)
                     /locus_tag="ACHKIR_001958"
     CDS             complement(31541..32023)
                     /locus_tag="ACHKIR_001958"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000128190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO9989705.1"
                     /translation="MTCEQTILDMCCGSRMFWFDKEDKRAVFSDIRAEQHELCDGRQL
                     VISPDLIADFRALPFADNTFPVVVFDPPHLERVGDNAWMRKKYGRLNKETWRDDLRDG
                     FAEAFRVLRPHGVLIFKWNETQIPVSNILALTDEKPAIWQRTGKADKTHWVIFVKGVE
                     "
     gene            complement(32013..32234)
                     /locus_tag="ACHKIR_001959"
     CDS             complement(32013..32234)
                     /locus_tag="ACHKIR_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HFO9989706.1"
                     /translation="MSFKYRDSPLYYRAAREALRLEQSGEYDRAAKVWAKANRQSRNE
                     LNQDWSERRSDFCLMQNMREKRKAVSDDL"
     gene            complement(32240..32443)
                     /locus_tag="ACHKIR_001960"
     CDS             complement(32240..32443)
                     /locus_tag="ACHKIR_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9989707.1"
                     /translation="MTVIKTHTGTVITRTGEKQVQLHQNATTWVAGPKEYYYKDTGRR
                     GGAVGVRTRLVLSSIRPIEAKQE"
     gene            complement(32449..34512)
                     /locus_tag="ACHKIR_001961"
     CDS             complement(32449..34512)
                     /locus_tag="ACHKIR_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HFO9989708.1"
                     /translation="MSNILWGDLETYCDIPINNGTHAYAEGVEVMLFAWAINDGPVNV
                     WDITAGGGIPHGLYEAIAAPETLLYFHNSHFDRTVLRYAMPRLAPPVERWRDTMVQAL
                     AHGLPGSLGELCEVLGVPQDKAKDKEGKALIQLFCKPRPKNSKLRRATSKTHPEEWRR
                     FVAYAGLDIEAMREVYKRLPKWNYQGTELALWHRDQQINDRGVCMDMQLARAAIEAVD
                     QEQKRLAKRTQEMTDGEVQAATQRDALIKHIVESYGVELPDMQRSTLERRIADPDLPS
                     AVKELLAIRLQASTTSTSKYKALMKGVSHDGRLRGTLQFCGASRTGRWAGRLFQPQNL
                     PRPSLKQEQIDEGIEALKAGCADLLFDNIMELTSSALRGCIIAPTGKKLVVSDLSNIE
                     GRMLAWLAGEEWKLNAFREYDAGTGPDLYKLAYAKAFDITPDDVDKHMRQIGKVMELG
                     LGYGGGVSAFITFALVYGLDLDELANAALPNIPRDVIREAKSWYNESVKRKSTFGLSE
                     RVFIACDSLKRLWRRAHPATCDFWYELERTVRTAIATPQKTLYCGYLKIRRDGAWLRI
                     QLPSGRAVCYPSPVIEKGNITYMGVNSYSRKWQRLKTYGGKLVENVTQAAARDVLAGN
                     MPLIEDAGYSIVLTVHDEVITEAPDTEDFNDKALSALLSTNPEWAPDIPLNAGGFEAY
                     HYRKD"
     gene            34574..35263
                     /locus_tag="ACHKIR_001962"
     CDS             34574..35263
                     /locus_tag="ACHKIR_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000215799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9989709.1"
                     /translation="MTVGNKRQPNGLPEYYCHRGNDIKDAIVRDFIIQPVAHLKLITG
                     QTKTSYSGYPLTDQYYCFSFKKRSDENCTGAFFCGRDVAKDFIRRIGCTELPLFNPMK
                     SDTSAAIERSGSGSGSSGITVKPNADKKLFIDAARLLMTLWDSTSKGQTPLASVLNEA
                     ISGGIDKPPEIRHVVAVNTMLGNTKNTLTGLLDKARVKYPDVLFRDFDFTPLNEIVKR
                     SKSYNKHPPCY"
     gene            complement(35257..35898)
                     /locus_tag="ACHKIR_001963"
     CDS             complement(35257..35898)
                     /locus_tag="ACHKIR_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001520089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nmad5 family putative nucleotide modification
                     protein"
                     /protein_id="HFO9989710.1"
                     /translation="MSQPVLTNHLKTQIINNALEKAGIPKRKSALRAARVEWAERVRL
                     AAIGGVEKEAEILKNIKKIESMVTKIPDSLKTNSSLIRKDCCIYLNLAGARANIYFNG
                     NYRGYESGAPEHINKIAPAEFTLLGEDPLVTEFYSFDALYTQIKSDETEIIQNVSAAL
                     NKVRTVKQLLDVWPEAKELLPTDAPPVPLAPAVHRETLNEMIGLPTNTVSDTQ"
     gene            complement(35950..36498)
                     /locus_tag="ACHKIR_001964"
     CDS             complement(35950..36498)
                     /locus_tag="ACHKIR_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2815 family protein"
                     /protein_id="HFO9989711.1"
                     /translation="MKIKLNNVRLAFPALFEAKTVNGEGDPRFSAVFLMSPKHPQLEE
                     IRKAMKQVAKEKWGEKWESIYNQLEKKLNLCLHDGDEKAEYEGFPGNFFLNAANKARP
                     AVLDRDRSPLIQADGRPYAGCYVNAVIDIWAQDNNFGKRINASLGGVQFLRDGDAFAG
                     GGVASADDFDDISEGADAEALI"
     gene            complement(36514..37815)
                     /locus_tag="ACHKIR_001965"
     CDS             complement(36514..37815)
                     /locus_tag="ACHKIR_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001386185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2800 domain-containing protein"
                     /protein_id="HFO9989712.1"
                     /translation="MPDVHAQLSPSSAHRWLRCAGSLALEATQPDKETTFAIEGTAAH
                     ALAEKVLRNRQSHPEHYAGCNVSMFLGSYPLRENPDDTSGPQVDDEMVEAVGRYVDTV
                     WTLAKNNELLVEQRVDFSHITGVEESFGTADGIIIAGSELQIHDLKYGKGVRIDAEQN
                     EQLQLYALGALEQFSMLYDFETVRLFIHQPRLNHVSEWSLTVQELQSFGERALEAATS
                     AILVLNIAECEGIETLPLENFIPGEKQCRFCKAKAICTAQKMQHLQTAASDFEDLTKP
                     VSEIITNASARVPLLTIEQLAEIYSQADFIESWLKAVRDRVHNELNAGHPVPGFKLVT
                     GKQGNRAWSDEEAARALLKDQFRYKTEEVFDLKLISPTKAEKLIKKASPRRWSKVEAL
                     ITRADGKPTVVPESDPRPALNINPVNDFDDVSDDTLAADLI"
     gene            complement(37818..38720)
                     /locus_tag="ACHKIR_001966"
     CDS             complement(37818..38720)
                     /locus_tag="ACHKIR_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9989713.1"
                     /translation="MSLETSLELNNQLLAQHNALLERLIRTMASGIVMHPDTISRVQE
                     CRDTATEAENMSAAMTLDDLEFSDVIALAGFYPVATPITEDMLQRAVAYRDAEGDKRV
                     VQIDALDSALQGVKRARALLKPALLDLSRNILKFWDDLPTIGERRAFAEQLLDAPADG
                     RDEVKPKRASNKDGERTGPFYVKNVSGTAASELHTLRKLNEMLKKGHIEINRVEYLQL
                     QEEFARRDAANSSQNNDAKDDHTIDFAALRKQAEGLILQLAKGGYRAEAIAILEKQGA
                     RKLGEVTDENLAEVITLAEKALEG"
     gene            complement(38717..38866)
                     /locus_tag="ACHKIR_001967"
     CDS             complement(38717..38866)
                     /locus_tag="ACHKIR_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9989714.1"
                     /translation="MKPEHLYRLTGRDVLRYRRKNFDLMTGLAVATALGLIITFILLV
                     ARTTV"
     gene            complement(39594..40223)
                     /locus_tag="ACHKIR_001968"
     CDS             complement(39594..40223)
                     /locus_tag="ACHKIR_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000049986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HFO9989715.1"
                     /translation="MNLANRAKKRRTELNLTQVEVAKRAGISQQSIEAIENGKTLKPR
                     NLLALASALECDPKWLLLGGDVVTDFNYGARRVPVLSYVQAGAFTSAELLREEGDFEY
                     ILTTAELSESSFALRIRGDSMEPEFKEGDIVIIDTDVYPTPGEFVAACNGSHEATFKK
                     YRPVGIGAKGEEDFELVPLNSDYPICRSWEKPIKIIGTMVEHRIFRRKR"
     gene            40331..40555
                     /locus_tag="ACHKIR_001969"
     CDS             40331..40555
                     /locus_tag="ACHKIR_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015571354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO9989716.1"
                     /translation="MTSSTLASRIKERRKALGITQTSLAESVGMRQQSVQYLESGRAT
                     RTGFILELAKVLKCDPDWLLNGENSEQQKV"
     gene            40559..42778
                     /locus_tag="ACHKIR_001970"
     CDS             40559..42778
                     /locus_tag="ACHKIR_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapE domain-containing protein"
                     /protein_id="HFO9989717.1"
                     /translation="MPDNKPWGATPDEWFHFDLVLGMTDRLLPVVSNPCAELSPNSKL
                     SHNFGKTPSRYNGQRKVVGIPDWANYVVTENDFSRWSNEPDYGICVRTGDGVVALDCD
                     INDAGMQEIVRNIILSCLGELPPRRWRADSHKCLYLIAVDGDYRKRGHRLEGENKQIE
                     LLAKGQQFVACGTHPAGERIQWDCGLPGEPLKITSEQLESLWQRLADNLPVKDSYTAG
                     AGRQRDLSCVDPSATDDVADWLDANGWTLSVSSDGSRNLKPFRDESEYSDGCSETSIK
                     YFPKGTGGFEQGHFKSMHNTDAGLTDADWLDAYGYRVADFEDLTVAEDGDKPEYLPWP
                     KFSRDKNDRIEGTVTNVLLALRRPDLCGVQIRLDEFRDEIILTTPRGKNLRLRDEFYT
                     MLHSRLECLGFKKFEEAAIRRAVRLVAYENRFDSLKDWIANLPAWDGVPRIDTFFCRH
                     WGIEANAYTHAVGRYWWTLLAGRALVPGIKGDMAVILVSQQGKNKSEGIRAMAPTPEH
                     YMELDFGKKGEERIREMRGHNVIELGEMRGMDKRGIGDVRVTISTRADRNRGLFREHY
                     VTLPRRCGFIGTSNSDTPLTDTEGNRRWLPMTIPDETDGKFIAQGIADEKIQLWAEAV
                     AVFKRDGIAWENAETLAKSILGDYEVKDEVWMSCVAEWLELPQDLYDMAADGTKKNCE
                     RVPLTSKDVLVEAIGFKAPQVKRSDEMKIAAIMKKLGYKNKQLQSLPGKPRSWEKCK"
     gene            43084..43365
                     /locus_tag="ACHKIR_001971"
     CDS             43084..43365
                     /locus_tag="ACHKIR_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012540180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9989718.1"
                     /translation="MNTTCARLPSADTLAEQAAKTASGWMSDAVIAIDELFGKGYAKQ
                     HPELVAAFMQTAGLDEAAMHLRGLVLANQRIDDTLEVLSEHIMLLNEPQ"
     gene            43434..43856
                     /locus_tag="ACHKIR_001972"
     CDS             43434..43856
                     /locus_tag="ACHKIR_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013813178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFO9989719.1"
                     /translation="MRRDMQLVLERWGRWAASEEYCSMVDWPSMSVTSRDVTDSGKPG
                     CSDEDGAVIDTCIAHMSMVCPYDDLLILGLRFIGGLSTRAIAEAIDRSHLSVRTSLKA
                     SEAFLEGALTLQGVRLDMDPEVVLPERVVCAQKNMLWL"
     gene            43942..44037
                     /locus_tag="ACHKIR_001973"
                     /pseudo
     CDS             43942..44037
                     /locus_tag="ACHKIR_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024321.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
     gene            44026..44102
                     /locus_tag="ACHKIR_001974"
     tRNA            44026..44102
                     /locus_tag="ACHKIR_001974"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:44060..44062,aa:Arg,seq:tct)
     gene            44663..>45033
                     /locus_tag="ACHKIR_001975"
     CDS             44663..>45033
                     /locus_tag="ACHKIR_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFO9989720.1"
                     /translation="MTFKHYDVVRAASPSDLADALAQKIREGWQPYGGPFSSYTDDGA
                     ALIQAIVAEGDVSTPVVVKPTGGEGAVISATSDPEYYFVVVLAGQSNSMAYGEGLPLP
                     ETYDRPDPRIKQLARRSTVTPG"
BASE COUNT        10941 a        11013 c        11665 g        11414 t
ORIGIN      
        1 cggtactaat gaaccgtgag gcttaacctt acaacgccga aggtgttttg gcggatgaga
       61 gaaagatttt cagcctgata cagattaaat cagaacgcag aagcggtctg atgaaacaga
      121 atttgcctgg cggccgtagc gcggtggtcc cacctgaccc catgccgaac tcagaagtga
      181 aacgccgtag cgccgatggt agtgtggggt ctccccatgc gagagtaggg aactgccagg
      241 catcaaatta agcagaaggc tatccgtcag gatggccttt ttgcgctgga gcagaataaa
      301 tgccggatgc gacgctggcg cgtcttatac tcccacataa gctagattca gcagcgaata
      361 cggcttcccc aacgtgccca cctccatacg tgtcctcctt accagaaatt tatccttaag
      421 ctcctcaata accattttcc tgctaactaa attcgtggtt aaggttgcat aatgatatgc
      481 aacaaatgta taacattttc tttaccaata gaaataaaca aaagcgaccg acaaaagcat
      541 cggattacgg caggagacat aatggcatgg ctgaaagtac tgtaacggca gacagcaaac
      601 tgacaagtag tgatactcgt cgccgcattt gggcaattgt gggggcctct tcaggtaatc
      661 tggtcgagtg gttcgatttc tatgtctact cgttctgttc actctacttc gcccacatct
      721 tcttcccttc cgggaacacg acgactcaac tactgcaaac agcaggtgtt tttgctgcgg
      781 gattcctgat gcgcccaata ggcggttggc tatttggccg catagccgat aaacatggtc
      841 gcaaaaaatc gatgctgtta tcggtgtgta tgatgtgttt cggatcgctg gttatcgcct
      901 gcctcccagg ttatgaaact ataggtacgt gggctccggc attattgctt ctcgctcgtt
      961 tatttcaggg attatccgtt ggcggagaat atggcaccag cgccacctat atgagtgaag
     1021 ttgccgttga agggcgcaaa ggtttttacg catcatttca gtatgtgacg ttgatcggcg
     1081 ggcaactgct agctctactg gttgtcgtgg ttttacaaca caccatggaa gacgctgcac
     1141 ttagagagtg gggatggcgt attcctttcg cgttaggagc tgtgttagct gttgtggcgt
     1201 tgtggttacg tcgtcagtta gatgaaactt cacaacaaga aacacgcgct ttaaaagaag
     1261 ctggatctct gaaaggatta tggcgcaatc gccgtgcatt catcatggtt ctcggtttta
     1321 ccgctgcggg ctccctttgt ttctatacct tcaccactta tatgcagaag tatctggtaa
     1381 atactgcggg aatgcatgcc aacgtggcga gtggcattat gactgccgca ttgtttgtat
     1441 tcatgcttat tcaaccactt attggcgcgc tgtcggataa gattggtcgt cgtacctcaa
     1501 tgttatgttt cggttctctg gcagccattt ttaccgttcc tattctctca gcattgcaga
     1561 acgtttcctc gccttatgcc gcttttggtc tggtgatgtg cgccctgctg atagtgagtt
     1621 tttatacttc aatcagtgga atactgaagg ctgagatgtt cccggcacag gttcgcgcat
     1681 taggcgttgg tctgtcatat gcggtcgcca atgctatatt tggtgggtcg gcggagtacg
     1741 tagcgttgtc gctgaaatca ataggaatgg aaacagcctt cttctggtat gtgaccttga
     1801 tggccgtggt ggcatttctg gtttcactaa cattgcaccg taaaggaaaa gggatgcggc
     1861 tttagtgacg ggtcagttgc cagacggtat agccggtgct tgcaccggcg atatcccagg
     1921 caaaatcctt ccagctccag ccgctcccct cagggcggct atcccaaagc tcttttgacg
     1981 cccccagact gacagagaac atcaatccaa acatggcact gcgatcccgg ctcatcccct
     2041 gatgctgtgc atattcattt ccggcggcgg aaagcatcgc cgaggcgata aagtgttgag
     2101 ctttatcctg cccgctccag ctatcgttag ccatatggct gcatccagaa agtaacaata
     2161 gcgaacagac aaaaagaata cgcaaaacgt ggcctccaaa acaaaccccg tacaggacgg
     2221 ggttgtgatt acaggatgcg gctaattaat cggtcgatgc ggatacggcg caaacgacgg
     2281 atgagtttac gaaccttcac cggataatcg gcaatacttt gcagatcgcg atagtgctta
     2341 acgatggtgg tatgctcgcg gatcagctcc agttctttct ctcgctgtgg cgcaagctcc
     2401 agttgcggat cgtggatcaa aatggcgttt tccagatcca gacgccaggc gcgcgggttc
     2461 aggttattac cggtgatcaa catccactta tcatcaaccc acatcccttt taggtgatag
     2521 gtgttgtcgt catctttcca taaccgaacc actagctggt cagtattgac gtaatactgc
     2581 aaacggctca ggaaacgacg cagattgatc tcatagagat aaggcaatgc gccaattatc
     2641 ttgaaaggtt catcttccgg aatgtagaag tcgttcgcgg ttttatcacc aacaataatt
     2701 tcgacctttt tcccttcgcg cagcaactgg ataatattgc gcacaaggat tgctggcagg
     2761 ttgaagtatg gcgtacagat ggttagtttc tgctcggcac aaggcataag atggaaaatg
     2821 gtcttgttca acagactcga tttccccagc cccactagcg gcgttacgga aagctgatcg
     2881 ttgtcggcat cgccctggaa atgataagcg gcatcacgca gctcctggcg gaacaggcga
     2941 atgtcgttct tgatttccgg gctttttggc cgattaacat catccagacg attaacgccg
     3001 cggccattca taatattctg tgtaacccat tcaaacataa tgtctgacat cttacggtta
     3061 cggattagat gataacggtc gtagcgatat ttatcgtgct gatgcaggta aacatcgttc
     3121 aggctggcac cgctataaag tacgctatcg tcgatgataa agcctttaaa gtgcagaaca
     3181 ccaagggctt cacgggtatt gattggaacg ccataaaccg gaacatctac gcccggattt
     3241 tcctgcgcca tgcggcagta ccagtcagcg ttagtgttag atgccgcagc gccaatgcgt
     3301 ccacgttgtg cacgatgcca gtcgaccagc acccgcacat ccagttccgg acgctgcctt
     3361 ttagcctcat acaacgcgtt cagaatgcct ttgccaccgt catcctgttc gagatacagg
     3421 gcaacaatgc aaatgcgctg cttcgcgctg gctatttttt ccagcaacgt ctcccggaag
     3481 tcggcgggag cgtaaaagaa atcgacatca tcaactgatt gagaaatctt gggtagttgg
     3541 gcaaggtgtt gttgatgttt attacgctta aattttgaca acatcacagt gcatttcttc
     3601 tctgttcatt gaagggtcct ctgtgcaatg cagacgacat aagcgggcaa taataacacc
     3661 agtcccgatt aagtggtcaa catttccagt accttactca tgattcctcg cgctgggcaa
     3721 gatttagcgt aagcccaacg atcccctctt cgagctggat atcaacgtta aacccgagct
     3781 tgcgggccag cgccaccatg ccacgattgt ttggcatcgt aataccattc agacgttgta
     3841 gtccgtgatc tcgcgtatag gtaatcaact tttccattaa gcgtcgacct aagcctaacc
     3901 ctttgagatc cgagcgaacc agcacggcaa attcggcatc gatgttatca gggtcggaga
     3961 tcgcacgcgt gacgccgagg atctcttctg tttgatcaat acgtcgtacc gccacaaaag
     4021 ccatttcccg atcgtagtcg atctgcgtca tgttggctaa atcttcatgg gtaaattcgt
     4081 tgatctcgct aaagtagcgg taataaagat cttctttggt gactcgcgaa atgaattgct
     4141 gaagctgtgg ctcatcttct ggcaaaatcg ggcggaacaa gcagcgttca ccgtttttca
     4201 attctaccca ttcttccagc tgatgcggat aagggcgcac tgccagccga ctctcgttat
     4261 cgccttcaaa cggcgcgata tccagcgtga catccagcgc ggtaaattca ctgccagaag
     4321 ccagcaaagg atgaatatcc agacgctgaa tttccgggca atcgacaatc aagttagaaa
     4381 cctgcaccag aagctggctc aagcctgcaa catctaatgg gcgtagcgca ctgcgcgcac
     4441 gaatcttttt acttttgatc ccctgaataa ccagatagcg ggccaggttc atgttcagcg
     4501 gcggtagtgc gacgacggct tgatcttcag gacgccactc cacaccgcct tcgcccagca
     4561 tgatcaacgg cccgaaaacc ggatcgtgct caaccacaac ccgcaactcc tgagcgccag
     4621 cacggttagc catactttgc accaacaggc cgtggatccg cgcctgcggc caggccattt
     4681 ttacgcgatc gaaaatagcg tttgccgctt gctggacttc attagctgta cgcaggtaga
     4741 gcattacgcc ctgaacttcc gatttatgtg gaatatccgg cgaacgcaat ttcagcgcca
     4801 ccggataacc aatctgttcg gcaatatgca ccgcttcggt gctatcgctg gcaatccagg
     4861 tggggagcgt gttcatgcca tacgcttgca ggatgggctg aacttcatgg gtatcgagcg
     4921 acgtagcccc ttcggcaatc gcctgttgca acagaagatg cgcttctgcg gtattagagg
     4981 tcagattgct gggcaacgcc ggcgtttcgc gtagttgctt ctgattacgt cggtactcca
     5041 ccatatgcat aaaagcagtg atggttcctt ccggcgtgcg ataggtcggc agcccggctt
     5101 cactgaataa tcgtcgtgcc tcttgcgagg agtgctcgcc gcaccagttc gtcagcagag
     5161 aaacatactt gctgcgggga tgatgcttta ccgcttcaat taatgcttgc gcactttctg
     5221 ttgcgggagc agcggcgctg ggcgaatgaa taaccatcag cgcgtcaaaa tcctggctgt
     5281 ggagcagaat atccagcgtt ttaatatagt gctcactgct ggcgtcatcg cgtagatcga
     5341 gcgggttaga gattgccaca tgttctggca gtgcatcgcg cagtttctgg caggtttctt
     5401 cgcttagcgt tgccagcttg ccattgcgtg accataaggc atccagcgcc agcgcggcag
     5461 gcgcagcacc gttgctgata atcatcagcc ggtcgccacg cagcgggcgc atatggctga
     5521 gggtttccac cgccgaaaac agctcgtgag tatcctgtac ccgcaacaaa ccggcacgct
     5581 gaatagccgc atcccacgcc ggatccattc ctgccgtggt gttcaacaga cgttgagccg
     5641 ccgggctacg tccgctttta atcaccagaa tcggtttgtt gcgagaggca ctacgggccg
     5701 ccgaaacaaa gcgtcgtgcg tcgcttaact gttcgagata gagcaggatg gcgctggttt
     5761 tactgtcgcg ggccagatag tcgagcaatt catcaacgtc gatatccagg ctgtcgccga
     5821 gcgcaataaa gtaggaaaag cccatctcac gctgttgcgc ccagtcgagg atggtattgg
     5881 agacggcagc cgattgcgaa ataaacgcca gcttgccgcg tttaatcggc acaggcgaaa
     5941 agctggcatt cagaccttgc cagggagcca gtaaccccag actgtttggt cctagcaggc
     6001 gcatgttatg gcgcagggcg caggcgcgga gatcttcatg ttgcgatgcc ggggcggaaa
     6061 gaataatgca ggttttacag cctttctcgc cgagctcttc cagaagagca agattacggc
     6121 tggcattggt acataaaacc gcaaggtcgg gtgtaaaggg caagctggca atatccggcc
     6181 aggccaacac acccaacacc gctttccagg ctggcgtcac cgggagtacc ggtccgttaa
     6241 agcctcccgc cagcaggtta cgcatcatca ggtaacctgc tcgattggat ttcatcgatg
     6301 cgccaattac cgcaatcgat tttggtcgca gtagtgcttc cagtcctcgc tgactcatac
     6361 cggtctcccg cagaagtgac cgaatgattt taaacgcttt ctaactgttc tgctgtgatg
     6421 ctacccagat gttgcgtttt tcctgccaga tagcgcgttt taaaacgagt gaaatgctcg
     6481 cctaagcccg ttgcggcttc ggtatcgccg gccatatcta acagtgcgat ggctacctcg
     6541 gcagtacaat attggccttc agcctgggct tcacgcaggc gataggcaga aagccgggaa
     6601 agatcgacgg aaatgacggg aagattatcc agatacggac ttttacgaaa catcttgcga
     6661 gcttcaggcc aggtaccatc gagcatgata aatagcggag gcttaccggc aggtggtgtg
     6721 aagatcactt cccgttgctc atcagcatac gaggcgggaa agaccaccat tggctgatag
     6781 tccgggtttt gtaccagatc cagcaaatct agcgagggtt cggtacgcga ccattgaaac
     6841 gcaacggtat caggcaaaat atcagcaatc agacgcccgg tattactggg cttcattggc
     6901 tcggtgtcga acatcagcaa acagaagcga ctttttgctt gtgctggggt aattgtcgaa
     6961 cagagacata atgtctctgg caaaagacag cgttggcagc gacgaacgcg attaccgcgg
     7021 gcaagaaaag gacgtgttgc gcgcgcaata cgctcggcgc gtaactggag aacagcgttt
     7081 tcggtcataa gagagcgtcg aaaaaacgcc attgtcgcag aggagaaaac ggggcacaag
     7141 atgcgccccg gtaagattaa agagattcgt tcagccagct atcaaacggc gctttcggta
     7201 ctgcgccatt aagcatgtcg acaacctgac cgtttttgaa aatcatgatc gtcggtatac
     7261 tacgaattcc aaagcgactg ctcaattcac gttcagcttc ggtattcact ttcacaaagc
     7321 gcactttacc gctacgctct tgcgcgacat cttcaaaaat tggtgcgaaa ttacggcagg
     7381 ggccgcacca cggtgcccag aagtcgatca ccacaggtag atcatctttc agcaatttgt
     7441 cgagcgtttc accggtcgca ttaatcacct ctccgtcaaa caagtcgtga ccgcagcgtc
     7501 cgcattttgc cgcatcttcg atccgatcgt cgggaatgcg attgatggcc tgacaatggg
     7561 tacaaacggt attcataact aacctcggga tgagtaagga gacttatcgg caacgacgcc
     7621 ggtatgtttc taatatgtta catattatcg gggacattgt tccagacaac aatctgtttt
     7681 attcaatagg tatgatagtc gcaggctttg gtacgaaatc atggcgaacc gcgcggactt
     7741 cgggtaatct gcgcgcttcg cgcagcgctg gtggagaaaa gcatgaacga tgaaatgaaa
     7801 ggtaaaagcg gcaaggtcaa agtgatgtat gtccgcagtg atgatgattc tgataaacgt
     7861 acccacaacc cgcgtaccgg gaaagggggc gggcgtccag gaaaatctcg tgctgacggt
     7921 ggccgtcgcc ccgcccgcga tgacaaacag agtcagcccc gtgaccgcaa gtgggaagag
     7981 tcgccgtggc gcacagtttc ccgcgcgccg ggtgatgaga cgccggaaaa ggccgatcac
     8041 ggtggcatca gtggtaaaag ttttattgat ccggaagtgt tgcgtcgtca gcgtgcggaa
     8101 gaaacccgcg tctacggcga aaatgcctgt caggcactgt tccagagccg tccggaagcg
     8161 attgttcgcg cctggtttat ccagagtgta acgccgcgtt ttaaagaagc cttgcgctgg
     8221 atggcagcaa accgcaaagc gtaccatgtg gtagatgaag cggaactgac aaaagcgtca
     8281 ggcacggaac atcacggcgg cgtttgcttc ctgatcaaaa agcgtaacgg tacaaccgtg
     8341 ctgcagtggg taagccaggc aggcgcgcag gattgtgtac tggcgctgga aaacgaatct
     8401 aacccgcata acctgggtgg catgatgcgc agctgcgcgc acttcggcgt gaaaggcgtt
     8461 gtggtgcagg atgcggcact gctggagtcg ggggcggcta tccgtaccgc agaaggcggc
     8521 gcagagcacg ttcagccgat tactggcgac aacattgtta acgtgctgga tgatttccgt
     8581 caggcaggtt acaccgtagt gaccacttcc agcgagcagg ggaaaccgct gttcaaaacc
     8641 agtctgccag cgaaaatggt actggtgctg ggtcaggaat atgaagggtt accggatgcc
     8701 gcacgcgatc cgaacgattt gcgcgtgaag attgatggta ctggcaacgt tgccgggcta
     8761 aatatctctg ttgcaaccgg cgttttgctt ggtgaatggt ggcgtcagaa taaagcctga
     8821 taaatcagcc gggtggcaac gctgccatcc ggcgttttcc tgcaaattta ctcactttct
     8881 gccggtaata ctggcatcca gtcaatcggt ttctcgccac gttgttccag ccactgattt
     8941 gccagcacaa aatggttgca gccaaagaac ccacgatgcg cagaaagcgg tgatggatgc
     9001 ggcgctttca gtatgtgatg gcgttgctta tcaataatcg cccctttctt ttgcgcatgt
     9061 gagccccaca acaaaaacac cacgtcttcg cggtgctggt taatcaggct gattacttta
     9121 tcggtgaagg tctcccagcc gaggctggcg tgggaatgtg cctgacctgc gcgaaccgtc
     9181 aacacagtat tgagtagcag aacgccctga cgcgcccagc tttcaagata accatgatta
     9241 gggcgggtga aaccgggaat agtattttcc agctctttat acatattcag cagtgacggc
     9301 ggtgtcgcaa tgccggggcg aacggaaaat gccagaccat gcgcctgtcc cggtccgtga
     9361 taaggatcct ggccgagaat caccacttta acgtcaccca actctgtaaa gcggaacgcg
     9421 ttaaagacat ctttttgtgg cggatagata gtgacgccgg actgccgctc gctggcgacg
     9481 gtctgtaggg tattaagaaa atagggttgc tgcttctctt cagccagcac atcatgccag
     9541 gttaattcgt ttgccatctc gctctcccgc gaatcttcaa tccgcctagc ttaactgctt
     9601 ctttctacag aacaaaatca tgctcatttt ttagacttaa acgagtgcaa aaaaatgtaa
     9661 attggtaaaa tttcacacct gcataagttg atgtaaaaca ataaaatcca tttgcaacca
     9721 tacttgatgt gtggttttta ttgatttaaa tcaaagattc aagggtgttt gaggagtata
     9781 tatacactca agcaacaatg gttttaccaa ttggccgcga caggctgaac aaatcaaata
     9841 attttgccgg ggaggcatca catgattaca ggtatccaga ttactaaagc cgctaacgac
     9901 gatctgctga actctttctg gctgctggac agcgaaaaag gcgaagcgcg ttgcatcgtt
     9961 gcgaaagcag gttttgcaga agacgaagtg gttgcagtaa gcaaactggg tgacattgag
    10021 taccgtgaag ttccagtaga agtgaaacca gaagttcgcg ttgaaggtgg tcaacatctg
    10081 aacgtaaacg ttctgcgtcg tgaaactctg gaagatgcag ttaagcatcc ggaaaaatat
    10141 ccgcagctga ccatccgtgt atccggttat gcagttcgct ttaactctct gactccggaa
    10201 cagcagcgcg acgttattgc tcgtaccttt actgaaagcc tgtaaggctt aagtagcaag
    10261 ttactaagcg gaagagggcg acttcttccg ctttttttcg ttaatagaaa atgcgtaccg
    10321 cgcaatagac cagcagcagc gcgagcacga tatttaactg gcgaccatac tggcgaaaca
    10381 atcgctgaaa caggtgcccc gccagcgccc agcacacatt gccaaacgtc ccaatcatcg
    10441 ccagcaaaac gctgacgcca attatccagc ttagcgcctg cgtttgcggc aggacaaacg
    10501 tcgacagtgc tgtaatgcca tacaaaatga ttttgacgtt cacaaactgc aaagcaaagc
    10561 tggcccaaaa actgattggt tttgtctgaa gcccatcttc ctttgttgga ctggtagcga
    10621 ttttccacgc cagccagaca atatatgccg cccccgccca actcaacaga tgcactgctg
    10681 ccgggtcgat aacagccagt gaaaacgaaa ttcccgcgca tagcaacatg acgattaaaa
    10741 atcccagact catccctgcc agcacgcgag tactctgacg aaatccgtgc gttgtggctg
    10801 agctaagggc gagaatattg ttcggtcctg gcgtcatggc ggtaatcagg gtgtaagtcc
    10861 aaaaagcact taaaagggtc ggtgtcactt gttatctcct ctgttttggt agacagtacc
    10921 cgacacggaa catttaaaaa aatgaatgtt tttacattct aaattcgata ttttcgatag
    10981 gtttggggtt atggatctgc gccgttttat tacacttaaa accgtggtgg aagagggttc
    11041 ctttttgcga gcttcgcaaa aattgtgctg cacacaatcg acggtgactt ttcatattca
    11101 gcagcttgag caggagttct cagtccagtt atttgagaaa attggtcgac gaatgtgcct
    11161 tacccgcgaa ggaaaaaagc tgctgccgca catttatgaa ctcaccagag tgatggatac
    11221 ccttcgtgag gctgccaaaa aagagtcgga tccggacggg gaactacgcg ttatttcagg
    11281 cgaaacgttg ctctcttatc gaatgccgca ggtgttgcag cgttttcggc aacgcgctcc
    11341 aaaagtgcgt ttatcgttgc agtcgctgaa ctgttacgtg atccgtgatg cgctactgaa
    11401 tgatgaggcc gatgtcgggg ttttttatcg cgtagggaat gatgatgctt tgaaccgacg
    11461 agagttgggt gaacaaccgc tggtgctggt ggcttcaccg caaattgcag atgtcgattt
    11521 tactgagccg ggaagacata acgcctgtag ctttattatc aacgaaccac aatgtgtctt
    11581 ccggcagata tttgagagca cgctgcgcca gcgacggatc acggtggaaa acaccattga
    11641 gcttataagt atcgaaagca tcaagcgttg tgtcgcggca aatatcgggg tcagttacct
    11701 gccgcgcttt acagtggcaa atgaacttgc gagtggcgaa ttaatcgaat tgccttttgg
    11761 cgaacagtcg cagaccatta cggccatgtg cgcacaccat gctggaaaag cggttagccc
    11821 ggcgatgcac acttttattc agtgtgttga agagtgtttt ttgccgggat aaaaacaatg
    11881 ccgggcttaa acccggcatt gagattactc ttctgtcgtt tgcggtggca cgcccgttcc
    11941 gcttggttta cggcgcttac caatattttt agtgtcgcga tggcgttttt tcacccgcgg
    12001 cttctctttc tctttagctt ttttcttctc ggcacgttta gccaacactt tcttcgatgg
    12061 cttgccggtc tgcttctcgc ttggcgcacg cgttttcgga cgtaactcat caattacgcg
    12121 agctttaatt ggctcttcaa tataacggcc aactttgccc agcagcagat ggtcatgggc
    12181 ttccaccagc gaaatcgcgg tgcctttacg accggcgcgc gcggtacgtc cgatacggtg
    12241 caaataagta tcgccactgc gcggcatatc gaagttaaag acgtggctga cgtcaggaat
    12301 gtcgataccg cgcgcggcaa catcggtagc aaccagtacg tttacgcggc cttcggtcaa
    12361 gcgtttaatc gcttcgttac gcttgccttg taccatctca ccttcgagat agcagttgtt
    12421 gatgcctgct tcgcgcagcc agtttgccag ctcatgcaca cgctcacgct tacgcacaaa
    12481 cacaattgag cgggtcgctt ccggctgttt taacagatgc accagcaacg cggttttatg
    12541 ctcaagatca tcggcgcggt aataccactg atgaattttt ttgcgctcac gggtggacgg
    12601 attggcagaa acctccaccg gatcttccag cagacgctcg gcaaagtcct gaatcgcatc
    12661 gccttccaga gttgccgaaa agagcagggt ctgtttacgc cagcgcgttt cgccagcaat
    12721 atgttcgata tcctgggcga agcccatatc cagcatacgg tctgcttcgt cgaggatcag
    12781 cgtttcaacc gcgcggcaat cgaagttctc ttcttttatg tattgcagca gacgtccggt
    12841 cgtggcgacc acgatgtcct gattttcgct gaacacttcc gcgtggttca tataggctac
    12901 gccgccggtg atggtggcaa tatccagatg cgtatgtttc gccagttcgc gggcatgatc
    12961 ggccacctgc atcgccagct cgcgagttgg ggtgaggatc aaaatacgcg gcggaccgga
    13021 tttcttacgt gggaaatcga gcaggtgctg caacgctggc agcagatacg ccgccgtttt
    13081 accggtgcct gtcggcgcag aaccgagtac atcacggcca tcgagcgcag gcggaatggc
    13141 ggcagcctga atggcggtcg ggcgagtgaa acctttatcc tggagggctt ccagcaggct
    13201 ttcgtcgagt tcaagttcgg aaaaagtcgt tacagtcatg ttctacctct gtgtggggcg
    13261 ctgattatag acgttacggc tgcaatcttc atctgtttgt atggatatcg cttctcgggt
    13321 attgttctca cccggtgcta tcgccgtttt tcctgcaagg ttttattttc atgtcacagt
    13381 ctacatccgt gcttcgtcgt aatggattta cttttaaaca gttttttgtt gctcacgatc
    13441 gttgtgcgat gaaagtggga acggatggca ttttgctggg cgcatgggca ccggtggctg
    13501 gggtaaaacg ttgccttgat atcggcgcgg gtagcgggtt gctggcatta atgctggcgc
    13561 agcgaaccga tgacagcgta atgattgatg cagttgaact ggaaagtgaa gctgccgcgc
    13621 aggcgcagga aaatatcaac cagtccccgt gggcagagcg gattaacgtc catacggcgg
    13681 atattcagca gtggatcaca cagcagacag tacgcttcga tttaatcatc agtaacccac
    13741 cttactatca gcagggagta gagtgcgcga cacctcaacg ggaacaggct cgctatacca
    13801 ctacgcttga tcacccatcg ttgctgacct gtgcggcgga gtgcattacc gaagagggat
    13861 ttttctgcgt ggtgctgcca gagcagattg gtaatggttt tactgaactg gcattaagta
    13921 tgggctggca tttacgtttg cggacggatg tggcggaaaa cgaagcgcga ctgccgcatc
    13981 gggtgctgct ggcattctcc ccgcaggcgg gagaatgctt tagcgatcgc ttagtcattc
    14041 gtgggccaga ccagaactat tccgaagcgt atacggcgct gacccaggct ttttatctgt
    14101 ttatgtaatg attgccgggg gaaaggatcg acggaccgga atgggtgagc agttccggat
    14161 aatccagcgt gaaatgcaac ccccgactct ctttacgcat cattgcacag cgaacaatca
    14221 actcggcaac ctgtaccaga ttacgcagct ccagcaaatt atttgagacg cggaaatggg
    14281 cgtaatattc gtctatttct tgctggagca tggttatccg ccgcagggcg cgttccaggc
    14341 gcttcgttgt gcgcacaatg ccgacgtaat cccacataaa cagacgtagc tcgtgccagt
    14401 tatgttgaat caccacccgt tcatcagggt tctcaacgcg gctttcatcc cacggcggta
    14461 acgtactgac gccgtgggca taaggcatac atctggtgat atcttctgcc gccgaccagc
    14521 catagaccag acactccagc aaggagttag aggccatgcg gttagcgccg tgtaagccgg
    14581 tataactcac ttcgccaatg gcatacaaac cctcgacgtc cgtacgccca tgatcatcca
    14641 ccatcacacc accgcaggta tagtgtgcag caggcacaat cggtactggt tcttgtgtga
    14701 gatcaatccc cagcccgagc aatttttcat aaatcatcgg gaaatgctgg cgaataaaat
    14761 cggcgggctt atggctgatg tcgaggaaca tacaatccgc gccgaggcgt ttcatttcat
    14821 ggtcaatagc gcgggcgaca atatcgcgcg gggccagttc gccgcgatca tcaaaatcgg
    14881 gcataaaacg cgtaccatcc gggcgcttga gataagcgcc ttcgccgcgc agtgcttctg
    14941 ttaacaggaa attgcgtgcc tgtgggtgat acagtgcggt agggtggaac tgattaaatt
    15001 cgagattggc aacccggcag cctgcgcgcc acgccatagc aatgccatcg ccagaagaaa
    15061 tatccggatt ggtggtgtac tgataaacct tcgacgcacc gccggttgcc agtaccactg
    15121 cttttgcgtg gcaggtttcc accgtttctt tatttcggtt ccatacccac gcgccaacaa
    15181 cccgtcgcgt gcccggcagg ccaattttgt cagaaacaat cagatcaacc gcgttgctgc
    15241 gctccagcac gcgaatattc ggatggttca gcgccttgct caccagcgtg gtttctactt
    15301 ctctaccggt ggcgtcggcg gcatgaagaa ttcgacggtg actatgtcca ccttcacggg
    15361 tcagatggta gctttctgca ccattcggtt gaatgtgggt atcaaacaac accccctggt
    15421 cgattagcca ttgcacacag gatcgtgcat tgctggcgac aaattcaact gcatgacgat
    15481 cgcaaatacc agccccggca atcaatgtgt cttccacatg cgagtcaatg ctgtcagtct
    15541 catcaaacac ggcggctata ccgccctggg cataaaatgt tgaaccttcc gttaccgggc
    15601 ctttacttag aacgatgacc tgatgctggt cagccaggcg tagcgccagt gaaagtccag
    15661 ctgcgccgct accgataatc aacacgtcac atgaatgttc agggagagta ttcattttct
    15721 ttgtttaatt tactaaacat ggtttggtca gcatagcatc atgttgtgcg attaaacacc
    15781 tgctatttta atatttgtta cagtgactaa acacgctgac tcagggcggc gagaaaaaga
    15841 gaagttactg gctggtggag gattaggtgg tgaaataaaa aggccgttgg gttactcttc
    15901 aggcagttaa atgggcattt ctacacagat aatgcgatgt tcagattctg tagacttata
    15961 atgatagata atgatccgtc tacagcatga caaacaaaaa cagatgcgtt acggaacttt
    16021 acaaaaacga gacactctaa ccctttgctt gctcaaattg cagctaatgg agtggcgttt
    16081 cgatagcgcg tggaaatttg gtttggggag actttacctc ggatgagcga gcagttaacg
    16141 gaccaggtcc tggttgaacg ggtccagaag ggagatcaga aagcctttaa cttactggta
    16201 gtgcgctatc agcataaagt ggcgagtctg gtttcccgct atgtgccgtc gggtgatgtt
    16261 cccgatgtgg tacaagaagc ttttattaaa gcctatcgtg cgctggattc gttccgggga
    16321 gatagcgctt tttatacatg gctgtatcgg attgctgtaa atacagcgaa aaattacctg
    16381 gttgctcagg ggcgtcgtcc accttccagt gatgtggatg ccattgaagc tgaaaacttc
    16441 gaaagtggcg gcgcgttgaa agaaatttcg aaccctgaga acttaatgtt gtcagaagaa
    16501 ctgagacaga tagttttccg aactattgag tccctcccgg aagatttacg catggcaata
    16561 accttgcggg agctggatgg cctgagctat gaagagatag ccgctatcat ggattgtccg
    16621 gtaggtacgg tgcgttcacg tatcttccga gcgagggaag ctattgataa caaagttcaa
    16681 ccgcttatca ggcgttgacg atagcgggat actggataag ggtattaggc atgcagaaag
    16741 aacaactttc cgctttaatg gatggcgaaa cgctggatag tgagctgctt aacgaactgg
    16801 ctcataaccc agaaatgcag aaaacctggg aaagctatca cttaatccgt gactcaatgc
    16861 ggggtgatac tcccgaggtg ctccatttcg atatctcttc acgcgtgatg gccgccattg
    16921 aagaagagcc agtacgtcaa ccggcgacat tgatcccgga agcccagcct gcgccgcatc
    16981 aatggcagaa aatgccattc tggcagaaag tacgtccgtg ggcggcacag cttacccaaa
    17041 tgggcgtagc cgcatgcgta tcgcttgcag ttatcgttgg cgtccagcac tataatggac
    17101 aatctgaaac gtcccagcag cccgaaacgc cggtatttaa tacactgccg atgatgggta
    17161 aagccagccc ggtaagcctg ggagtacctt ctgaagcgac cgcaaacaat ggtcaacagc
    17221 agcaggtaca ggagcagcgt cgtcgcatta atgcaatgtt gcaggattac gaactgcaac
    17281 gccgactcca ctctgaacag cttcagtttg agcaggcgca aactcagcaa gccgctgtac
    17341 aggtgccagg aattcaaact ttaggaacgc aatcgcagta atgaagcaac tttggtttgc
    17401 catgtcatta gtgacaggta gcctgttatt ctctgctaac gcctcggcca ctcccgcgtc
    17461 cggggcgtta ttacagcaga tgaacctggc cagtcagtca ctgaattacg agctgtcatt
    17521 catcagcatc aataaacagg gtgttgagtc tctgcgttat cgacatgcac gcctcgataa
    17581 ccgtcctctt gcacaattgt tgcaaatgga tggcccgcgc cgggaagtgg tacagcgcgg
    17641 caatgaaatc agctattttg aaccgggact tgaaccgttc acgcttaatg gcgattacat
    17701 tgttgattct ctaccatcgc tcatctatac cgatttcaaa cgcctttctc cttactacga
    17761 ctttatctcc gtcgggcgca cgcgtattgc tgatcgtctt tgcgaagtca ttcgtgtggt
    17821 tgcccgagat ggtacacgct acagctacat cgtgtggatg gacaccgaat cgaaattacc
    17881 gatgcgggtt gatctccttg atcgcgatgg tgaaacgctg gaacaatttc gcgtgattgc
    17941 ttttaatgtc aatcaggata tcagcagcag tatgcagacg ctggcgaagg caaatttgcc
    18001 gccgttgctt tctgttcctg taggtgaaaa agctaaattc agctggacgc caacctggtt
    18061 gccacagggt tttagcgaag tttccagtag tcgacgtccg ctaccgacga tggacaacat
    18121 gcctatcgaa tcacgtctct attccgacgg attattcagc ttctcggtaa acgttaaccg
    18181 cgctacgcca tcgagcaccg atcagatgtt gcgcaccggg cgcagaaccg tcagtacaag
    18241 cgtacgtgat aacgccgaaa tcaccattgt cggtgaactg ccgccgcaaa cggcgaaacg
    18301 cattgccgag aatattaagt tcggggcagc gcaatgatca aagagtgggc taccgtcgtc
    18361 tcctgggaaa acgggcaggc gctggttagt tgtgatgtta aagcctcatg cagcagctgc
    18421 gcttcacgtg ccggttgcgg tagccgcgtg ttgaataaac ttggcccgca aaccacgcat
    18481 accattgtcg taccctgtga tgagccgtta gtgccggggc aaaaagtaga attaggaatc
    18541 gccgaaggca gcctgcttag ctccgcatta ctggtttata tgtcgccgtt ggtgggacta
    18601 ttcctcatcg cttcactatt tcagctactc tttgcttcag atattgcagc attatgcggt
    18661 gcgattctcg gtggcgttgg tggattcctg attgcacgtg gctgctctcg aaagtttgct
    18721 gcccggtctg aatggcaacc gatcatctta agcgtggcct tgccgcctgg ccttgtgcga
    18781 tttgaaacat cttcggaaga cgcgagccag tgatttcact ggctttgttg cattttgcat
    18841 aacaaatgcg aggacgtttc cagataccac aatccatacg gtatgaacat ttcctcgcct
    18901 caatgttgta gtgtagaatg cggcgtttct attaatacag acgttaagct cagaacagcg
    18961 acttctaaaa gcctggttaa ccgggcatta aggcacaata atcatacttt atgaagaata
    19021 tacgtaactt ttcgatcata gctcacattg accacggtaa atcgacgctg tctgaccgta
    19081 ttatccagat ctgcggtggc ctgtctgacc gtgaaatgga ggcgcaggtt ctcgattcca
    19141 tggatcttga gcgtgagcgt ggcattacca tcaaagcgca aagcgtgacg ctggactaca
    19201 aagcgtctga cggcgaaacc tatcagctta actttatcga caccccgggc cacgtagact
    19261 tctcctatga agtttctcgt tcgctggctg cctgtgaagg tgcattgctg gtggtcgacg
    19321 ccgggcaggg cgtagaagcg caaaccctgg caaactgcta caccgccatg gaaatggatc
    19381 tcgaagtggt gccggtactg aacaagattg acctgccggc agccgatcct gaacgcgtgg
    19441 cggaagaaat tgaagatatc gtcggcatcg acgccaccga cgcggtgcgc tgttcagcga
    19501 aaaccggcgt tggcgtacag gacgttctcg aacgtctggt gcgcgacatt ccgccgccgg
    19561 aaggcgatcc ggaaggcccg ttgcaggcac tcattatcga ctcctggttc gacaactacc
    19621 tgggcgttgt ttcacttatc cgtattaaaa acggcaccct gcgtaagggc gacaaagtga
    19681 aagtcatgag taccgggcag acctataacg ccgaccgtct gggcatcttc acgccgaagc
    19741 aggttgaccg tactgaactg aaatgcggcg aagtaggctg gctggtatgt gcgattaaag
    19801 atatccacgg cgctccagta ggcgatacct taacgctggc gcgtaatccg gcagaaaagg
    19861 cgctgcctgg ctttaagaaa gtcaaaccgc aggtatacgc cggtctgttc ccggtaagtt
    19921 ccgacgacta tgaagccttc cgtgacgcgc tgggcaaact cagcctgaac gatgcttcac
    19981 tgttctatga gccggaaagc tccagcgcgc tgggctttgg tttccgctgc ggcttcctcg
    20041 gcctgctgca catggagatc atccaggaac gtctggaacg tgaatacgat ctggatctga
    20101 tcaccactgc gccgaccgta gtgtatgaag ttgaaaccac gtcaagagaa gttatctacg
    20161 tcgacagccc atccaagctg ccagcggtaa ataacattta cgaactgcgc gagccgattg
    20221 cagagtgtca catgctgctg ccgcaggcat atctcggcaa cgttattacg ctgtgcgtag
    20281 aaaaacgcgg cgtgcagacc aatatggttt accacggtaa tcaggtggcg ctgacgtacg
    20341 agatcccgat ggcggaagtg gtgctcgact tcttcgatcg cctgaaatct acctcgcgtg
    20401 gttatgcgtc tctggattac aacttcaaac gcttccaggc gtccgacatg gtacgtgtag
    20461 acgtattaat caacggtgaa cgtgttgatg cgctggcgtt gatcacccac cgtgataatt
    20521 cgcaaaaccg cggtcgcgag ctggtggaga agatgaaaga tctgatccca cgccagcagt
    20581 ttgatatcgc cattcaggca gcgattggta cgcacatcat tgcgcgctcc accgtgaaac
    20641 agctgcgtaa aaacgtactg gctaaatgtt atggcggcga tatcagccgt aagaaaaagc
    20701 tgctgcagaa gcagaaagaa ggtaagaaac gcatgaagca gatcggtaac gtcgagctgc
    20761 cgcaggaagc gttcctcgcc attctgcacg tcggcaaaga caacaaataa cccttaggag
    20821 ttggcatggc gaatatgttt gccctgattc tggtgattgc cacactggtg acgggcattt
    20881 tatggtgcgt ggataaattc tttttcgcac ctaaacggcg ggaacgtcag gcagcggcgc
    20941 aggcggctgc cggtgactca ctggataaag caacgctgaa gaaagttgcg ccgaagcctg
    21001 gctggctgga aaccggtgct tctgtttttc cggtgctggc tatcgtattg attgtgcgtt
    21061 cgtttattta tgaaccgttc cagatcccgt caggttcgat gatgccgact ctgttaatcg
    21121 gtgattttat tctggtagag aagtttgctt atggcattaa agatcctatc taccagaaaa
    21181 cgctgatcga aaccggtcat ccgaaacgcg gcgatatcgt ggtctttaaa tatccggaag
    21241 atccaaagct tgattacatc aagcgcgcgg tgggtttgcc gggcgataaa gtcacttatg
    21301 atccggtctc aaaagagctg acgattcaac cgggatgcag ttccggccag gcgtgcgaaa
    21361 acgcgctacc ggtcacttac tccaacgtgg aaccgagcga tttcgttcag accttctcac
    21421 gccgtaatgg tggggaagcg accagcggat tctttgaagt gccgaaaaac gaaaccaaag
    21481 aaaatggaat tcgtctttcc gagcgtaaag agacattggg tgatgtgacg caccgcattc
    21541 tgacagtgcc gattgcgcag gaccaggtgg ggatgtatta ccagcagcca gggcaacaac
    21601 tggcaacctg gattgttccg ccgggacaat acttcatgat gggcgacaac cgcgacaaca
    21661 gcgcggacag ccgttactgg ggctttgtgc ctgaagcgaa tctggtcggt cgggcaacgg
    21721 ctatctggat gagcttcgat aagcaagaag gcgaatggcc gactggtgtg cgcttaagtc
    21781 gcattggcgg tatccattaa tagccatctt cgttcacgtt gtcgccgtta tggcgacaac
    21841 gtgaattatt tatgagataa atctcccgtg gctaacgaca tcccccgtcg ttgtgtatag
    21901 aatattcccc cgaagtttaa ggttggcacc tccaggttgc cacggcacac gaaacagcgt
    21961 tggtccccat ataccggtaa actgaaactg cagtgaagca gttagccgag ccatgtatat
    22021 caggtctgtt tcgtgtgctg aattgttgac gcatttattt attggtatcg catgaacccc
    22081 atcgtaatta atcggcttca acggaagctg ggctacactt ttaatcatca ggaactgttg
    22141 cagcaggcat taactcatcg tagtgccagc agtaaacata acgagcgttt agaattttta
    22201 ggtgactcta ttctgagcta cgttatcgcc aatgcgcttt atcaccgttt ccctcgtgtg
    22261 gatgaaggcg atatgagccg gatgcgcgcc acgctggtcc gtggtaatac gctggctgaa
    22321 ctggcgcgcg aatttgagtt aggtgagtgc ttacgtttag ggccaggtga acttaaaagc
    22381 ggtggatttc gtcgtgagtc aattctcgct gacaccgtcg aggcattaat tggtggcgta
    22441 ttcctcgaca gtgatattca aaccgtcgag aaattaatcc tcaactggta tcaaactcgt
    22501 ctggacgaaa ttagcccagg cgataaacaa aaagatccga aaacgcgtct gcaggaatat
    22561 ttgcagggtc gccatctgcc gctgccaact tatctggtag tccaggtacg tggcgaagcg
    22621 cacgatcagg aatttactat ccactgccag gtcagcggcc tgagtgaacc ggtggttggc
    22681 acaggttcaa gccgtcgtaa ggctgagcag gctgccgccg aacaggcgtt gaaaaaactg
    22741 gagctggaat gagcatcgat aaaagttact gcggatttat tgctatcgtc ggacgtccga
    22801 acgttggcaa atccacattg ttgaacaaac tgctggggca gaagatctcc atcacctccc
    22861 gcaaggcgca aacaactcgt caccgcattg tggggatcca tactgaaggc gcgtatcagg
    22921 cgatctacgt tgatacccca ggcctgcata tggaagaaaa acgcgccatt aaccgcctga
    22981 tgaacaaagc ggcgagcagc tctatcggcg acgttgagct ggtgattttt gtcgtagaag
    23041 gcacccgctg gacgccggac gatgaaatgg tgctcaacaa actgcgcgat ggcaaagcgc
    23101 cggtaatcct cgcggtgaac aaagtggata acgtgcagga aaaagccgat ctgctgccgc
    23161 acttacagtt cctggcaagc cagatgaact tcctggatat tgtgccgatc tctgccgaaa
    23221 ccgggctgaa tgtcgacact atcgcagcaa tcgtgcgtaa gcatctgcct gaagcgaccc
    23281 atcacttccc ggaagattac atcaccgatc gctcacagcg ttttatggcg tctgaaatca
    23341 tccgcgaaaa actgatgcgt ttcctcggcg ctgaactgcc gtactccgtg accgtggaga
    23401 tcgaacgttt cgtctctaac gaacgcggcg gttatgacat caacggtttg attctcgttg
    23461 agcgtgaagg gcagaagaag atggttattg gcaacaaagg ggccaagatc aaaaccatcg
    23521 ggattgaagc gcgtaaagac atgcaggaaa tgtttgaagc gcctgttcac cttgagctgt
    23581 gggtaaaagt gaaatccggt tgggccgacg acgaacgcgc actgcgcagt ctcggttatg
    23641 ttgacgatct ttaagagtaa ctccgatgga aggctggcag cgcgcatttg tcctgcatag
    23701 tcgcccgtgg agcgaaacca gcctgatgct ggacgtcttc acggaggaat cgggtcgcgt
    23761 gcgtctggtt gccaaaggcg cacgctctaa acgctctact ctgaaaggtg cattacaacc
    23821 tttcacccct ctcttgcttc gcttcggtgg gcgtggtgaa gtcaaaacac tgcgcagtgc
    23881 tgaagctgtc tcgctggcgc tgccattaag cggtatcacg ctttacagcg gtctgtacat
    23941 caacgaactt ctctcccgcg tactggaata cgagacgcgc ttctctgaac tctttttcga
    24001 ttacttgcac tgcattcagt cacttgcagg tgccactggt acgccagaac ccgcgctgcg
    24061 tcgctttgaa ctggcactgc tcgggcatct gggctatggc gtcaatttta cccattgtgc
    24121 gggtagcggc gagtcggtag atgacaccat gacgtatcgt tatcgcgaag aaaaagggtt
    24181 tatcgcaagc gtcgttatcg acaataaaac gttcaccgga aggcagttaa aagcgttaaa
    24241 cgcgcgggaa tttcctgacg cagacacact gcgcgccgcg aaacgcttta cccgcatggc
    24301 gcttaagccg tatcttggcg gtaaaccatt aaagagcagg gaactgttcc ggcagtttat
    24361 gcctaagcga acggtgaaaa cacattatga atgatgagga ttgtcatggc tgaattactg
    24421 ttaggcgtca acattgacca tattgcaacg ctgcgtaacg cacgcggtac tgcttacccg
    24481 gatccggtgc aggcggcgtt tattgctgag caggcgggag cggacggcat taccgtgcat
    24541 ttacgtgaag atcgccgcca cattactgac cgcgatgtgc gcatcctgcg tcagacgctg
    24601 gatacccgca tgaatctgga gatggcggtg accgaagaga tgctggcgat cgccgttgag
    24661 acgaagccac atttttgctg tctggtaccg gaaaagcgtc aggaagtaac aaccgaaggc
    24721 ggcctggacg tcgcagggca gcgtgacaaa atgcgcgatg cctgtaaacg tctggcggag
    24781 gccgggatcc cggtttctct gtttatcgac gccgatgaag agcagatcaa agctgcggca
    24841 gaggttggcg caccgtttat cgagatccac accggttgct atgctgatgc caaaactgac
    24901 gccgaacagg cgcaagagct ggcgcgtatc gccaaagccg cgacctttgc cgctagtctc
    24961 ggtctgaaag ttaacgctgg tcacggtctg acctatcaca acgtgaaagc tattgccgcc
    25021 atccctgaga tgcatgaact gaatatcggt catgccatta ttggtcgtgc agtgatgacc
    25081 ggactgaaag atgcggtggc agaaatgaag cgtctgatgc tggaagcgcg tggctaatgg
    25141 caatattagg tttaggtacg gatattgtgg agatcgctcg catagaagcg gtgatcgccc
    25201 gatccggtga gcgcctggca cgccgcgtat taagcgataa cgaatgggca atctggaaaa
    25261 cgcaccacca gccggtgcgt tttctggcga agcgttttgc tgtgaaagaa gcggcggcaa
    25321 aagcgtttgg caccgggatc cgcaatggtc tggcgtttaa tcaatttgaa gtattcaatg
    25381 atgagctcgg caaaccacgg ctacggctat ggggtgaggc attaaaactg gcggaaaagc
    25441 tcggcgttgt aaatatgcat gtaacgctgg cagatgaacg ccactatgct tgtgccacgg
    25501 taattattga aagttaaaaa aataccgttc tctttataaa gtaaaacccc cgaactggta
    25561 aggaacgggg gttttcactt tacaacttta attttgtgca gcacacgatt ccaacagaaa
    25621 tgattatgga tcaatttcgt tatcctgtac aagcaacttg caatctctga cataaaaaac
    25681 gcgagccttt acgaagaaat cgacattgat aaaaagatta acgtgaccgc tttttgataa
    25741 gtacattaaa atcggtttcg ttattggcaa ttttgctgtg ctttacacca tgccactgaa
    25801 ttcccccatt gaaacgagtg gtgtcgtcaa agctctggtg tggagtgcag cactcgattc
    25861 caataactcg cacgttcagt tttggggtga tgtaagggct aatctgaacg gctgcattcc
    25921 ttgtttaagg aaaaacgaat gactgattgc cgatacctga ttaaacggat tatcaaaatc
    25981 atcattgctg ttttacagct gatccttctg ttcttataac acaaggaaac gtacttaagg
    26041 tgcgtccggt gaaccagtcg gacgcgcctt taataactat aaataagtgt ctgggcagat
    26101 actatataaa ttaacttagt gaataattat gctaatgtca tcaatcaaat aaatataata
    26161 gcgttaaggt ttctctataa aataattaac actctacatc cagggtagaa tattaaattt
    26221 tatccgcgtg atgcataagc acaaatttat cccacaactg ttcttccgtc tcgacatgtt
    26281 gcggatcctt cacaatagta ttggggatcg ggcacacctt ctggcaggtt ggtgtctcgt
    26341 agtgccctac gcattcggta cacttatcgc tgttaatctc gtagatatga tcgcccattg
    26401 aaatcgcctc attcgggcat tcgggttcac acatatcaca attaatacag cgtttagtaa
    26461 ttagtaaaga catttcaatg aattaccgtt aaatcatttt aaaatcagta agttgtatcg
    26521 agtttgtatg ctttactgtc attaacttac tgtatgttga tccagtgtat ttaaccttga
    26581 taaactcagt ccagcaacac aaaaccgcaa cacattgcat tttgtcccgt aaaaaagact
    26641 tgtatgtgtg tgcttgtttt ctgcgcctac gcagataagg attgagaatg ccgcgcactg
    26701 taacacataa tccggatagc cccaataatg acgatgtttt agccgcatct gaaaaatggg
    26761 acgcctgtaa acccccctat accagcgcac acatgaaaat ctgtgttgct gccgccaaaa
    26821 tcatcctcgc tgcttccggc gtggctcgcc gttccaaata cgaaaaagag aactatctcc
    26881 gtatcgattt cagcaaagcc ggtaaggtta cattttacgc cgagtttcca aaaaagatgg
    26941 gcctcaaggg taaaaagctc ggcgagtggc cggagctcgc tatccagctg gcgcgcgaaa
    27001 aagcgctagg tatggctgac tgtggcttgc gggcagagtc agtacatgca gcgctggaaa
    27061 tgtaccggga tgacctcaaa gccaaagtcg cccggcagaa gctgagcccg gacagtttca
    27121 caacctacgg ggtgcgtatc gaccggatta aagcaacgtt cggcgagcgt gaggtgttca
    27181 gcgacgtaac atacaatcgg ctggtggaag tgctggacga gtggatcgcc actcgctcga
    27241 acaataacgc cctggagttg tttgccgagc tccgtcggtt ctggaagttc tgcgcaccta
    27301 ctctttgcaa cggccgcaat gttgccgcca gtctgccaga tgattatgtt tcctcccgcg
    27361 tacagaaacc tacccccaca cggcttttta ccgatattga atcaatcgcc cgactctggc
    27421 tcaatgttgc tgcctgcacc tctgtacacc agaagaatgc tgttcgcttc atgatcatca
    27481 ctggcgttcg tccgattaat gtccataacc tgcgctggga ctacgttcac gaggaggctg
    27541 gtgaaattgt ttatccggaa ggggttatcg gcatgcgagg ggctatgaaa acacaaaagg
    27601 ctttccgcct gccgataacg cctgagatcc ggcggattat cgacgagcag aaagcctggc
    27661 gtgattcagt tcctgagtgc aacagggatt atgtattttt gcagccacgt gatccaatgc
    27721 agccattttc aaaacgatca ctggataagc tggtgaaaac atacagcccg gacggggctg
    27781 taaaaggaat aaaacatgat gggactgtta aagggaaaga cggtgcattt aatacgatgt
    27841 gccgtaaatt ccttaagagc aatgttattg ccttgatgaa ggaaagaggc tattcccgat
    27901 cagaccgaag ggaaatcagc ctcctttgcc ttcaccactc cagcaagtca gatgacccga
    27961 tggcagaaca ttacgacttt tctgatgaga ttttgcagga agagattgcg ttaaagcgcg
    28021 aagctttcga ggctcacgag cggagcatac ttgcgcaggt ggcattgcta cggcggcgag
    28081 gttaatactg gctgcgacac ttttgaataa aagcgtcgac atttcggcgc tcataacgaa
    28141 ctacttttgc actgaaacga attggtgcca ggatagcccg atgacgatgc ttaatattcc
    28201 actcacatag cgttttctgt gtaataccta acttttggca tacttcatcc ggggtgagta
    28261 aatcgtcggg tttctcgctc atgctatacc tctctttttc atggcatcga gcaggatgtc
    28321 ctgcactgtt cgttttgagt tgcgccgctc catcaccatt tcgtccatag tgtcggccgc
    28381 aataatgtgg tgaataaata ccggacgatt gtgtccggcc tgtatctgcc tggtggggcc
    28441 gatacgttca ataatttgct gatactgctc caggtcccac cagtgtgaga aaaataccag
    28501 tatgtttccg ccgtcctgca tgttcagacc gtggcccgcg ctggctgggt gtgcaaagag
    28561 aacaggaatc tttccggaat tccagtcgcg cagtgtctgt ggatcttggt cgaggtgacg
    28621 accgcgaggg aatgctttaa gcaagcgttc aagatcgtgt ttccagtgat aagcaaccag
    28681 cacaggtgcg ccagctgctt cggtcagtat gctgtccagc gcctgtagtt tggtgtcatg
    28741 cagttctgac caacttccgg tgtcgtctgt gtatactgcg ccactggcga tttgaagaca
    28801 cttcagtgtc tttgccgcgg cgttcggtgc ttcgatgcct tcgccattca gctcgaggaa
    28861 catttccttt tccatttcac gatactgctg acgggccttc ggcggcatat ccacgcggat
    28921 tacgttatgg atggggtctt tgatatcgaa ccagtcggcc gcatccagcg agagggtcac
    28981 atcggctaac gctcgctgta tttcagcctg tgagtgagca aacggctcca gtttggtcca
    29041 gctctgcccc ggaaactgta tcgagttgaa ccagcgtgag gtaaacgcgc cgtaagtgcg
    29101 cccgagacgt tgcccctggt ccacaaacca caattgtccc cacaaatcta ccaggccgtt
    29161 cggtgctggc gtaccggtga gatttatcca gcgctggaca tacttatgcg ccacttttcc
    29221 cagcgccgcc gcgcgcttac caccacctcg cagccggaag gattttagcc gggtgctttc
    29281 atctggaatg acagtaccga acggccatcg ttctcccaat tcctcaacca gccagacaag
    29341 gttatcgtag ttgatggtga acacgctcgc gttgctgttc gccagcgctg cagagcgcgc
    29401 tttggcgtta ccaacaatcg gctgcacctc gatattgcgc agatgccccc atttcagcgc
    29461 ttcatccggc catgtgcttg ctgcaacgcg tagcggcgcg aggaccagcg cggggcgtgt
    29521 ttctgcccct gccataaaga ggtcttccag cgcagtgagc gttgccacgg ttttacccat
    29581 tcccatcccc gcccagatgt tgccgcgcag ggtttcgatt tggtgattga tgatcaggtc
    29641 ttgatacggg cggggggtga aaatttgtcc catagtgttt tagccagtag ggcgcgggta
    29701 atcccgcgca ataatgtcag attagatcgg tgtagtaggt gaagttgcca aactgcggat
    29761 gtctccagcg cttgcggtta cctttcggcg gtgtcgattc gtttaacagc ttttgagctg
    29821 cttgttcgat cgctgggagg ttaacgagca atccccgaac accggtgtct gaacgtaccg
    29881 gtatcctggc gaagccgatc aatttccggc atgttgaatc agataaacct gttttctatg
    29941 ctgctttgct gacggggaca tattcacctt caacatgaga taaatttgct ggtgcggctg
    30001 gttgagaata gccagttgca tcaagacagg ctttcattgt tgcttctgct acgacgcgag
    30061 caagccggac gatgtcgtcg ttgatctgaa ttttgggtgt ggaaggatgg gtaacaagcg
    30121 gttgtacttt acctgtttcc agctcacgcc agcgcttaac tactttatac cggtcgtggg
    30181 cgctataacc agttacaagc gttgtggaaa ggtcctgatc gagtagtatt tcatcaatga
    30241 cttcgcgacc attatagatc ttccttttga ctaaaaagtc tttattttca tgatggtaca
    30301 tttctgtact atgaaaaccc aactgctcaa gaagtgcttt aatatcgcgc acgactattt
    30361 tatgttcttt gctggtaagt tctgcaatct cgcgactgct catcatcggt tgcttaccag
    30421 tgcctgctag tgttaaagtt gtttcgaaca ttgaagtatt ctccattcag taaaagatgc
    30481 gctggtagct gcaactatca gcgcgttttg tttattcatc ccacggcttg tttgcagctt
    30541 cccttgcggc ctgctgcgca aaataaatca attcaaaagc taactgctct cctttttcca
    30601 gcgaactgag aagtctcgct gtttccagca tgtccgcgat atacccgaaa atatctgcct
    30661 gatgtaactg cgtgatctga atagccatag cgttaacctt gttttgtact cattgtgagg
    30721 actttattag tactcacaat gaggcttgaa gtcaacatgt ttgtgaggca tgttgttctc
    30781 acactttcaa gggatcatat atgagcaaaa gagacgaccc gcagttgagg gtacgcatac
    30841 cagaattgct aaaagaggct ttggagaaga aagcacgggc taataagaga acactgacct
    30901 ctgagatcgt tagccggttg gaatctacta ttcttcagga caaattactt catacatcta
    30961 gggggtttga ggaagctgct gatgagattc tcatactcag ggatcttctc gagaaactta
    31021 agacaacgta ccatcgagag tatcaggtcg aatgggttta tgaaaacaaa tacgagctta
    31081 ttgaagtgat ggaaaaactt aagaagttgc taaatcctga gaatgaatag tggattcatc
    31141 ctctaaactt ttacgtaacc gccgggtgat ttcggcggtt agtgttctgt cgttttctct
    31201 ggaaattttt tccagtgtgt ctttcaagtc ttgcgggatg cgtacccgca attgtgggtc
    31261 atcacgttta ctcacaatat tccctccaga tttttgctat ccagcaccac cacggtaaag
    31321 cccagctttc gcagacgttc atgctcgcgc aactggtcag gccgtggtgg cttgcctggc
    31381 gctttgcatt cgacaaagac gatgcgaccg ccgggtagca ggacaatgcg atccggtacc
    31441 gagcggcgac cgggtgacac gaacttaaag gcgaccccgc cagccttttt cacttcggcg
    31501 acgaggtgct tttcgataag gctttcacgt tcgtaggcca tcattccaca cctttgacga
    31561 aaataaccca gtgggttttg tcggcttttc cggttcgttg ccatatggcc ggcttctcgt
    31621 cggtcagcgc caagatattg cttaccggga tctgcgtttc gttccatttg aatatgagta
    31681 caccgtgggg ccgcaatacc ctgaatgctt cggcgaagcc gtcgcgaaga tcatcgcgcc
    31741 atgtttcttt gttgagccgc ccgtactttt tcctcatcca tgcgttatcg ccgacgcgct
    31801 cgaggtgcgg cggatcgaaa acgacgacag ggaaagtgtt atcggcaaag ggaagggcgc
    31861 ggaaatcagc tataaggtcc ggacttataa ccaactggcg accgtcgcaa agctcatgct
    31921 gctcggcgcg gatatcactg aacacggcgc gcttgtcctc cttgtcgaac caaaacatac
    31981 gagagccgca gcacatgtca agaatggtct gctcacaagt catcgctcac cgccttacgc
    32041 ttttcgcgca tgttctgcat cagacaaaaa tcagaccggc gttcgctcca gtcctgattc
    32101 agttcgttac gtgactggcg gttagccttg gcccagacct tcgctgcccg gtcatattcg
    32161 ccggactgct caaggcgcaa ggcctccctc gcagcccggt aataaagcgg actgtcccga
    32221 tatttaaatg acataggggt tactcctgct ttgcttcgat tgggcggatg ctgctcagca
    32281 ccagacgagt gcggacaccg acagcaccac cgcggcggcc agtgtctttg tagtaatact
    32341 cttttgggcc agccacccac gttgtcgcgt tttgatgcaa ttgcacctgc ttttcaccgg
    32401 tgcgggtaat aaccgttccg gtatgcgttt tgattacagt catagcgatt aatccttacg
    32461 gtagtggtac gcctcaaaac cgccagcgtt cagtgggata tcgggcgccc attcggggtt
    32521 agtggagaga agcgcggaaa gcgctttatc gttgaaatct tctgtgtcag gtgcttcggt
    32581 gatcacctcg tcgtgtaccg tcagcacaat gctgtaaccg gcatcttcga taagcggcat
    32641 gtttccggcc agaacgtcgc gggcggccgc ctgggtgacg ttctctacca gctttccgcc
    32701 gtaggttttg agtcgttgcc atttacgcga ataagagtta acacccatat aggtgatatt
    32761 ccctttttcg ataaccggag aggggtagca tacagcgcgt ccggatggta gctgtatgcg
    32821 cagccacgcg ccatcgcggc ggattttaag ataaccgcaa tacaatgttt tttgcggtgt
    32881 ggcgattgca gtgcggacgg tgcgctccag ttcgtaccag aaatcgcagg tcgccgggtg
    32941 cgccctgcgc cacaggcgct tgagcgagtc acatgcgatg aatacacgct cggacaggcc
    33001 aaaggtcgac ttacgtttaa ccgattcgtt gtaccagctt ttcgcctcgc ggataacatc
    33061 gcggggaatg tttggcagtg cggcgttcgc cagctcgtcg agatcgagac cgtaaaccag
    33121 agcaaaagtg atgaaagccg atacaccacc tccataaccc agaccgagtt ccatgacttt
    33181 accgatctga cgcatgtgtt tatcaacatc atctggtgta atatcgaaag cttttgcata
    33241 cgccagttta tataagtccg gacccgttcc ggcgtcgtac tctctgaatg cattcagttt
    33301 ccattcttct cccgccagcc atgccagcat acggccttca atgtttgaca agtcacttac
    33361 caccagtttt ttgcctgttg gcgcgataat gcagccacgt aacgctgaac tggttagttc
    33421 catgatattg tcaaacagca ggtctgcaca tccggctttc agtgcttcga tgccttcgtc
    33481 tatttgttcc tgttttagtg aaggacgggg aaggttctgg ggctggaata gccgtccggc
    33541 ccaacgaccg gtacgtgacg ctccgcagaa ctgtagcgta ccgcgtaagc gcccgtcgtg
    33601 gcttacgcct ttcatcagtg ccttgtattt actggtgctg gtagtactgg cctgcaggcg
    33661 gatagccagc agttctttca cggcagatgg taaatcgggg tcggctatac gacgttccag
    33721 ggtactgcgt tgcatgtctg gtagctccac accgtaggat tcaacgatgt gcttaatcaa
    33781 cgcgtcccgt tgtgtggctg cctgcacttc gccatcagtc atttcctgtg tacgctttgc
    33841 caagcgcttt tgctcttggt ctaccgcttc gatcgcagcg cgcgcgagtt gcatgtccat
    33901 gcagacgccc cggtcgttga tctgctgatc acgatgccag agcgccagct ctgtcccctg
    33961 ataattccac ttcggcagac gtttatagac ttcgcgcatt gcctcgatat ccagtccggc
    34021 gtaagcaaca aagcgccgcc attcttccgg gtgggtttta ctggtggccc gacgcagttt
    34081 gctgtttttc gggcgtggct tacagaacag ctggatcagc gctttacctt ctttgtcctt
    34141 cgctttgtct tgcgggacgc ctagtacttc gcagagttcc cccagagacc ccgggagacc
    34201 gtgcgccagc gcctgaacca tcgtgtcgcg ccaacgttcg actggcggtg ccagtcgcgg
    34261 cattgcataa cgcagaacgg tgcggtcgaa gtgagagtta tggaaataaa gcagggtttc
    34321 aggggctgcg attgcttcgt ataagccgtg tggaatacca ccaccggcag tgatatccca
    34381 cacgtttact ggcccgtcgt tgatagccca tgcgaaaagc atcacttcaa cgccttcggc
    34441 atacgcatgg gtaccgttgt tgatcggaat gtcacaatag gtttccaggt cgccccatag
    34501 tatattggac ataaaatatc ctcatatggt atatggttaa atgctagata tcatgaatta
    34561 aaggagataa ctaatgactg tgggtaataa gagacagcct aatggattac ctgaatatta
    34621 ttgtcaccgt ggtaacgaca taaaagatgc gatagttcgg gattttatta ttcagcctgt
    34681 tgcacacctg aaacttatta ctggacaaac taaaaccagt tattccggtt atccgttaac
    34741 cgatcagtac tattgttttt catttaaaaa acgctctgat gaaaattgta caggggcttt
    34801 tttctgtggt agagatgttg caaaggattt cattcggcgt atcggttgta cagagctgcc
    34861 gttatttaat cctatgaaat cggataccag tgcagcaata gaacgttccg gttctggctc
    34921 aggtagttct ggtattactg tcaagccaaa tgctgacaaa aagctattta tagacgcagc
    34981 tcgtctgtta atgacgctgt gggattcaac aagtaaggga caaaccccac tggcgtcggt
    35041 tctaaatgaa gcaatttctg gtggtataga taaacctcca gaaattagac atgtagtggc
    35101 ggttaataca atgttgggta acacaaaaaa tactttaact ggcctgctgg ataaagcaag
    35161 agtgaagtat cctgatgtgt tattcagaga ttttgatttc acgcctctta acgagattgt
    35221 aaaacgttca aaatcatata ataagcatcc cccttgttat tgagtatcag acactgtatt
    35281 ggtaggaagg cctatcattt catttaatgt ctctcgatgt actgctggtg ctaaagggac
    35341 tggcggtgcg tctgttggaa gcagttcttt agcttcgggc catacatcca gtaattgttt
    35401 tactgtccga actttattta gagccgcgct gacgttctgt atgatttcgg tttcatcact
    35461 tttaatttgt gtgtagagag catcaaagct ataaaactcc gttaccagag gatcttcgcc
    35521 aagtagagta aattctgcag gtgctatttt attaatatgc tcgggggctc ctgattcata
    35581 acctctgtag ttgccattga aataaatgtt tgctctggct ccagccagat tgagatatat
    35641 gcagcaatct tttcgaatta aagaactatt ggtttttaat gaatcaggga tttttgtgac
    35701 catggattca atctttttta tattttttaa tatttcagct tctttctcta cgcccccgat
    35761 agctgcaagc cgaacacgtt cggcccattc aacacgagca gctcgtagtg ctgatttacg
    35821 tttggggata cccgctttct caagtgcgtt attgatgatt tgagttttaa ggtgattagt
    35881 taatacgggc tgtgacatta ttctttccct catgagatac cccagcacgt agctggggga
    35941 tgaatatttt taaattagtg cttcagcatc agcaccttcg ctgatatcgt cgaaatcgtc
    36001 agcgcttgcc actccgccgc cagcgaatgc atcgccgtct cgcaggaact ggactccgcc
    36061 gagtgaggca ttaatgcgtt taccgaaatt attgtcctgt gcccagatat cgataacggc
    36121 gtttacatag caccctgcat agggacgtcc atcagcctga attagtggcg aacgatcgcg
    36181 atcaagaaca gctgggcgcg ctttgttagc agcgttcagg aagaaattgc cggggaagcc
    36241 ttcatactct gctttttcat caccatcatg caggcacaga ttgagttttt tctccagctg
    36301 gttataaatg gactcccact tctcccccca tttttccttc gctacctgct tcatagcttt
    36361 acggatttct tccagttgtg ggtgtttggg agacattaaa aatactgcgg agaaacgcgg
    36421 atcgccttcg ccgtttacag ttttagcttc aaacagagca ggaaaagcta atctcacgtt
    36481 atttaattta atcttcatga aagtctcctt taatcagatg aggtctgcgg cgagcgtatc
    36541 gtcggatacg tcgtcgaaat catttacagg gttgatattg agtgcggggc gtgggtccga
    36601 ctcgggaacg acggttggtt taccatcagc tcgtgttatc agtgcctcga cttttgacca
    36661 acggcgcgga ctggcctttt tgataagttt ttcggctttg gttgggctaa tcagcttaag
    36721 atcgaaaacc tcctcggttt tataacggaa ctggtctttc agcagcgcgc gggcggcttc
    36781 ttcatcactc caggcccggt taccttgttt tcctgttacc agtttaaacc ccggtaccgg
    36841 atgtccggca ttgagttcat tgtgaacccg gtcccgtact gcctttagcc aggattcaat
    36901 aaagtcggcc tgactataga tctccgcaag ctgctcaatg gttaacagag gtacacgtgc
    36961 gctggcattg gtgattattt cgctgacagg ctttgtcaga tcttcaaaat cgctggccgc
    37021 tgtttgtaaa tgctgcattt tctgggcagt gcaaatagct tttgctttac agaagcggca
    37081 ctgtttttct ccaggtatga agttttccag cggtagtgtc tcaatgcctt cgcattcagc
    37141 aatattgaga acaaggatcg cactggttgc ggcctccagt gcccgttcac cgaaagactg
    37201 aagttcctgt acggttaaag accattctga aacgtggttg agccttggct ggtgaataaa
    37261 taatcttaca gtctcaaagt catacagcat gctgaattgt tcgagagcac ccagagcata
    37321 cagttgtagt tgctcatttt gttctgcatc aatgcggacg cctttgccat atttcaggtc
    37381 gtggatttgt aattcgctac cagcaatgat tatgccgtcg gcagttccga aagattcttc
    37441 cacacccgtt atatgtgaga aatcaacacg ttgttcaacc aatagttcat tattctttgc
    37501 aagagtccag accgtatcaa cataccggcc gacggcttcg accatttcat catccacctg
    37561 tgggccagat gtatcatcag gattttcgcg aagggggtat gagccgagaa acatagaaac
    37621 attgcatccg gcgtagtgtt ccgggtggct ttgcctgttt cgtagaactt tttcagcaag
    37681 cgcgtgcgct gcagtgccct cgattgcaaa agttgtttct ttatccggtt gtgtggcctc
    37741 cagcgccaga ctacccgcac accttagcca acgatgtgca gatgatggag aaagttgtgc
    37801 atgaacgtct ggcatgatta accctccagt gctttttcag ccagggtgat tacttcagcg
    37861 agattttcat ccgttacttc accaagtttc ctggctccct gtttttccag aattgcaata
    37921 gcttctgccc ggtaaccccc ttttgctaac tggaggatca acccttcagc ttgtttgcgt
    37981 agtgccgcga aatcaattgt atggtcatct ttggcgtcat tattctggct ggaatttgct
    38041 gcgtctctgc gtgcaaattc ttcctgcagc tgaaggtact caacacggtt gatctcgata
    38101 tggccttttt tcagcatctc gttcaacttg cgtaaggtgt ggagttcact ggctgctgtg
    38161 ccggatacat ttttgacgta aaacggcccc gtgcgttctc catctttgtt actggccctt
    38221 ttcggcttaa cttcatcacg cccatctgca ggtgcatcaa gtagctgctc ggcaaaagca
    38281 cgtcgctcgc cgatggttgg caggtcgtcc cagaacttaa gaatgttacg ggacaggtcc
    38341 aggagagcag gtttaagcag cgccctggct cgtttgacgc cctgtaatgc gctgtcgaga
    38401 gcatcaatct gaactactcg tttatcgcct tcagcatcac ggtaggcaac agcacgttgc
    38461 agcatgtctt ctgtgatagg ggtggctacc gggtagaaac cagccagtgc gataacgtcg
    38521 ctgaactcca gatcatccag tgtcattgcc gctgacatgt tttcagcttc agttgctgta
    38581 tcccgacatt cctgcactcg tgaaatcgtg tcaggatgca taacaatacc tgatgccatt
    38641 gtgcggataa gacgttcaag cagcgcatta tgttgtgcca gaagttgatt attaagttcg
    38701 agactggttt ctaaactcat actgtggtcc tcgctacaag gagaatgaaa gtgatgatca
    38761 gaccgagcgc agtggcaacg gccagaccgg tcatcaaatc gaagttttta cggcgataac
    38821 ggagaacatc gcgccccgtc agtcgataga ggtgttcagg tttcattggt tgtattcctt
    38881 ttttcatatc ggggagcacg ctgttgcgag tgcgctttca gacataaaaa agcccgtcac
    38941 gtgaggcggg caaagactac acacagcaat tacatggatg attcagttgg tattgtgcgt
    39001 acgaggtacc acaaggttga agcggacacg ccagtgcgtt gcaatggcgt acaggaaagc
    39061 accacattca gggcagtgct cataacattc gttgatttgg tatcgttcag gctttggtgc
    39121 ctgaagttgt gcttggcaag cagggcattc acctttgagc ggtttatatg ttttcacacc
    39181 gtttcctcat tcagttctct ttggtggttc ggctgctgcg gtctgagcgt tttacttgtg
    39241 catgatgttt tcctccattg gttcagacgc cccatcatcg gggcgtttca atttgcgtga
    39301 cttatcagct cgtcgcggtg ttgtcctcta cgcttaccgt acgcatacgg actcggcgct
    39361 tacctcgatc ccatcgggtg ccatttcatt ttgccaggag tactgcggct tacctgtcac
    39421 gcggttcagt ttgttaaaga gcgattactt attgcggtta aaaatacaag caaacctgta
    39481 agtgcgtcaa caggtattct ggtaataaaa tgcattatta acctaattac ttgtttttac
    39541 aggtaataaa aaaagagggc ctaagccctc tttgtttagt agtcaggatg cgattatctt
    39601 tttctgcgga atatgcgatg ttctaccatt gtccctatga tcttaattgg tttttcccag
    39661 gagcgacaaa tagggtaatc actattcaat ggaacaagtt caaagtcttc ctcaccctta
    39721 gcgccaatcc ctaccgggcg gtattttttg aacgttgctt cgtggctacc gttacatgcc
    39781 gcaacgaatt caccaggagt agggtatacg tcagtgtcaa ttataacgat gtccccctct
    39841 ttaaattcag gttccattga atccccacgt atacgtagcg caaatgaact ttctgatagc
    39901 tcagccgtgg tgagaatgta ttcgaaatca ccttcttcac gaagtagctc agcgcttgtg
    39961 aaagcccctg cttgtacgta gcttagaaca ggcacccttc gggctccgta gttgaaatct
    40021 gtaacaacat cgcccccaag aagtagccat ttaggatcgc attcaagagc tgacgctaaa
    40081 gcaagaagat tgcgtggttt aagtgttttc ccgttctcta tagcttcaat cgactgctgg
    40141 ctaatgcccg ctcgtttcgc aacttcgacc tgagttaagt ttagttctgt tctgcgtttt
    40201 ttagctcggt tggcaagatt catgttgact ccttttctgt cattgatgat tacagatact
    40261 tctgtatttg acaaacaagg ttgcctgtga ggtaattaca agtaaacctg taataatgag
    40321 ggcttactca atgaccagtt ctacccttgc ttcacgtata aaggagcgcc gcaaagcttt
    40381 aggtataacc caaacttcat tggctgaaag tgttggcatg cgccagcagt ctgttcaata
    40441 ccttgagtca ggacgcgcca cccgtaccgg tttcatcctt gaattggcaa aagttctgaa
    40501 atgcgatcct gactggctat taaacggcga aaattcagag cagcaaaagg tgtaacccat
    40561 gcctgataac aaaccctggg gagctacgcc cgatgagtgg ttccacttcg atctggtgtt
    40621 ggggatgaca gacaggcttt tacctgtggt gtccaatccc tgtgcagaac tgtcgcctaa
    40681 tagcaagctg tctcataatt tcggcaaaac tcccagtcgc tacaatggcc aacgtaaggt
    40741 tgttggtatc cccgactggg ctaactatgt tgttacggaa aacgattttt cccggtggtc
    40801 gaacgaaccc gattacggta tttgcgttcg tactggcgac ggggtggtgg ctctggactg
    40861 cgatatcaat gacgccggga tgcaggagat cgtacgcaat attatcctgt catgcctggg
    40921 ggaacttccg ccacgtcgct ggcgggctga cagtcataaa tgcctgtacc tgattgctgt
    40981 tgatggcgac taccgtaaac gtggtcaccg cctggaaggc gaaaacaaac aaatagagct
    41041 gctcgctaag ggccagcagt ttgtggcttg tggtacccat ccggcaggcg aacgtattca
    41101 gtgggactgc ggtttgcccg gcgaacctct gaaaattaca tctgaacagc tcgaaagcct
    41161 gtggcagcgt ctggcggata acttacccgt gaaagacagc tataccgcag gtgcaggacg
    41221 tcagcgtgac ctttcctgtg ttgatccttc cgcgacagat gatgtcgccg actggcttga
    41281 cgcgaacggg tggactctgt cagtcagctc tgatggttcc cgcaatctga aaccgtttcg
    41341 ggatgagtca gaatacagcg acggttgcag cgaaacatcc attaagtatt tcccaaaagg
    41401 gacgggggga tttgaacagg gacattttaa aagcatgcac aacaccgatg cggggcttac
    41461 tgatgccgac tggctggatg catacggcta ccgcgtggcg gacttcgaag atttgacagt
    41521 ggctgaagat ggcgataagc ctgagtatct tccctggcct aagttcagtc gtgataagaa
    41581 tgaccggata gaaggtaccg ttaccaatgt gctgctggcg ctgcggcgac cggatctctg
    41641 cggtgtacaa atccgccttg atgagttccg ggacgagata atactgacca ctccacgggg
    41701 gaaaaacctg cggttgcgtg atgagtttta taccatgctg cactctaggc tcgaatgtct
    41761 gggattcaag aaatttgaag aggcagcgat caggcgtgcg gtgcgcctgg tcgcgtatga
    41821 aaaccgtttt gattcgctta aagactggat tgccaatctt cctgcatggg atggtgttcc
    41881 ccgtatagat actttcttct gtcggcactg gggtatcgag gccaacgcct acacccacgc
    41941 agtcgggcgc tactggtgga ctttgctcgc gggtcgagct ctggttcctg gcattaaagg
    42001 ggacatggct gtgatccttg tcagccaaca gggtaagaac aagtccgaag gtatccgtgc
    42061 aatggctcca acccctgagc attacatgga actggacttc ggtaagaagg gagaggagcg
    42121 cattcgtgag atgcgagggc ataacgttat tgagcttggt gagatgcgcg gtatggataa
    42181 acggggtatt ggtgatgttc gtgtcaccat atccacccgt gcagaccgca accgcgggtt
    42241 gtttcgggag cactatgtca ctttaccgag gcgttgcggt tttattggta cttctaactc
    42301 cgatacgcca ttgacagata ccgaaggtaa ccgccgttgg ttgccgatga cgataccgga
    42361 tgagactgac ggcaagttta tcgctcaggg tatcgctgac gaaaaaatac agctttgggc
    42421 tgaagcagtc gccgttttta aacgtgatgg catcgcatgg gagaacgctg aaacactggc
    42481 taagtctatt cttggggatt atgaggtcaa agatgaggtc tggatgtcat gcgtcgctga
    42541 gtggttagag ttgccacagg atctgtacga tatggctgcc gacggcacga aaaagaactg
    42601 cgaacgcgtc ccccttacgt caaaagacgt attggtcgag gcgattggct tcaaagcccc
    42661 ccaggtaaaa cgcagtgacg aaatgaaaat tgcagcaatc atgaagaagc ttggttacaa
    42721 aaataaacag ttgcaaagtt taccgggtaa gcctcgcagc tgggaaaaat gcaaataact
    42781 acaagtttgt agatttttgc aggttgttac tcaacctact caacctcaca agggtaggtt
    42841 gagtaagaaa aatggtatta aaatcatgat attactcaac cactcaacct actcaactat
    42901 tttatataaa tatacgcgtg cgcgcacgca tgtatgtaag aattttattt ttccaaaagg
    42961 ttgagtaggt tgagtaagag tatgattttt atgtttttta ggttactcaa ccttttgccc
    43021 gtaaaaaaag gttgagtaaa gaaaacttgc agaaaatcac aaataaccaa acggagtaaa
    43081 aacatgaata caacctgtgc aagattacca agtgcggata cgttagcgga acaggcagca
    43141 aaaacggcaa gtgggtggat gtcagacgcc gtaatagcga tagatgagct ttttggaaag
    43201 ggctatgcga agcaacaccc tgaattagtt gctgccttta tgcaaacagc gggcttagat
    43261 gaagctgcaa tgcacttacg gggacttgtg ttagctaacc aacggattga tgacacactg
    43321 gaagtgcttt cggagcatat tatgctgctt aacgagcccc aataaacccg atgcggataa
    43381 acagacgtag ttctcagaaa attttttcgt agcaaaacgt agaggccagc gttatgcgta
    43441 gagatatgca acttgttctg gagcggtggg ggcgatgggc ggcgagcgag gaatactgtt
    43501 caatggttga ctggccctca atgtcggtta cttcccggga cgtaaccgat agtggcaaac
    43561 ccggttgctc tgacgaggac ggagcggtga ttgatacctg catcgcgcat atgagtatgg
    43621 tttgcccgta cgatgatttg ctgatcctgg ggttacgttt tataggcggc ctctcaacgc
    43681 gtgcaattgc tgaggcgata gaccgtagtc atcttagcgt acggacatcc ctgaaggcgt
    43741 ctgaagcatt tctggaagga gctctgactc tgcagggagt aaggttggat atggatcctg
    43801 aagttgtact acctgaaagg gttgtgtgcg cacaaaaaaa tatgttatgg ttatagcgtc
    43861 tataattgta tgcttactgc tgaaaggttc cgataacacc ggaacctttt ttcttgtctt
    43921 ctgctgggaa aaattaataa aacaggaaag aaaaacatga catcgcactg gttcagtgtc
    43981 agccagtggc agttaccaaa cgaggatgat tacagaaaac ttcatgcgtt gttagctcag
    44041 ccggacagag caattgcctt ctaagcaatc ggtcactggt tcgaatccag tacaacgcgc
    44101 cacattgatt tttctgtctc gcttcggtgg gcctttttgt cgctgaaaaa gaaaacaacg
    44161 ggtggttagc agagtatcag ttaatcattg aaatttttaa atgcctcaca atttcgtaag
    44221 ttgatgattg tctggctgat ggcgaatttg ttaaaaatcg catcgcatgg cgaatccccc
    44281 tgtgcggagg ggcgactggt gaaaaatcct tacttgtgat tcattatcga cgcgggttcg
    44341 gtggcaccgg gccgaactca ccgggaggca cccggcacca tgtacatcat gatacagata
    44401 cgcagcgtca gcccctctcc ggaggggctt ttttatggat aaaaaaagcc cgcgccagga
    44461 gacgcgggcg gcaaggaata aacaacaaaa cgtgaagtaa tatttcagct ggcgaataat
    44521 acccgacagt aatcactctg cgcaactgcg cggccttttt cgtattgcgg gctgtagtct
    44581 tccttctgtc attgtccttt aacttccgga cctcagcccg ctctttatct gattcagtac
    44641 actatcccgg ccgggaggat tcatgacatt taaacactac gatgtggtca gggcggcgtc
    44701 gccgtcagac cttgctgatg cacttgctca aaaaattcgt gaaggatggc aaccatacgg
    44761 cgggccgttt tcttcgtata cggatgatgg cgcagcactt attcaggcga ttgtcgcaga
    44821 aggtgatgtg agcacacctg ttgtggtgaa gccgacaggt ggagaaggtg cagtaatcag
    44881 cgccaccagc gaccccgagt attactttgt tgtggttctg gcggggcagt caaacagcat
    44941 ggcatatggt gaaggccttc cgctgccgga gacatatgac cgtccggacc cgcgtattaa
    45001 gcagctggcg cgccgcagta cggtgacacc ggg
//
LOCUS       DAZGQL010000031        43063 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.031, whole genome shotgun sequence.
ACCESSION   DAZGQL010000031 DAZGQL010000000
VERSION     DAZGQL010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 43063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43063
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.031"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(158..625)
                     /gene="yiaL"
                     /locus_tag="ACHKIR_001976"
     CDS             complement(158..625)
                     /gene="yiaL"
                     /locus_tag="ACHKIR_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HFO9989721.1"
                     /translation="MIFGHIAQPDPCRLPAAIEKALDFLRTTDFNALEPGVVEIDGKN
                     IYAQIIDLTTREAAENRPEVHRRYIDIQFLAWGEEKIGIAIDTGNNKVSESLLEQRDI
                     IFYHDSEHESFIEMIPGSYAIFFPQDVHRPGCILQTTSEIRKIVVKVALTALN"
     gene            complement(637..1635)
                     /gene="yiaK"
                     /locus_tag="ACHKIR_001977"
     CDS             complement(637..1635)
                     /gene="yiaK"
                     /locus_tag="ACHKIR_001977"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047559 - 3-dehydro-L-gulonate
                     2-dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HFO9989722.1"
                     /translation="MKVTFEQLKAAFNRVLISRDVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            1836..2684
                     /gene="yiaJ"
                     /locus_tag="ACHKIR_001978"
     CDS             1836..2684
                     /gene="yiaJ"
                     /locus_tag="ACHKIR_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HFO9989723.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWESHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DQGAIT"
     gene            2786..3259
                     /gene="ysaA"
                     /locus_tag="ACHKIR_001979"
     CDS             2786..3259
                     /gene="ysaA"
                     /locus_tag="ACHKIR_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFO9989724.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHTWTTAVACHQCEDAPCANVCPVDAISREHGHIFVEQSRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRATGPACVEACPTKALQCMDVEKVQRHRLRQQPV"
     gene            complement(3411..4664)
                     /gene="avtA"
                     /locus_tag="ACHKIR_001980"
     CDS             complement(3411..4664)
                     /gene="avtA"
                     /locus_tag="ACHKIR_001980"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HFO9989725.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRIKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMSEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPEDRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(4842..6869)
                     /gene="malS"
                     /locus_tag="ACHKIR_001981"
     CDS             complement(4842..6869)
                     /gene="malS"
                     /locus_tag="ACHKIR_001981"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFO9989726.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPTFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGEYTLQLDTRSGTPT
                     LMISIQNAVEPVASLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDAPAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGSEADLRTLVDSAHQRGIRILFDVVMNHTGYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIDGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASAALVEWKKANPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQDFNVLSYLSSHDT
                     RLFREGGDKAAELLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSAA
                     SVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            7189..8013
                     /gene="bax"
                     /locus_tag="ACHKIR_001982"
     CDS             7189..8013
                     /gene="bax"
                     /locus_tag="ACHKIR_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HFO9989727.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            complement(8121..9299)
                     /gene="xylR"
                     /locus_tag="ACHKIR_001983"
     CDS             complement(8121..9299)
                     /gene="xylR"
                     /locus_tag="ACHKIR_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="D-xylose enhances binding of XylR to the xyl
                     promoter and activates transcription; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose utilization transcriptional activator
                     XylR"
                     /protein_id="HFO9989728.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDGVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARARHILQVCEHLHIPVPEKLCVIGI
                     DNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEEMPLQRILVPPVRVIERRS
                     TDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRSNLEKRFKEEVGETIHAMI
                     HAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKKAYDTTPKEYRDVNSEVML
                     "
     gene            complement(9377..10558)
                     /gene="xylH"
                     /locus_tag="ACHKIR_001984"
     CDS             complement(9377..10558)
                     /gene="xylH"
                     /locus_tag="ACHKIR_001984"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HFO9989729.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            complement(10536..12077)
                     /gene="xylG"
                     /locus_tag="ACHKIR_001985"
     CDS             complement(10536..12077)
                     /gene="xylG"
                     /locus_tag="ACHKIR_001985"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015614 - ABC-type D-xylose transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HFO9989730.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRCQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALKKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            complement(12155..13147)
                     /gene="xylF"
                     /locus_tag="ACHKIR_001986"
     CDS             complement(12155..13147)
                     /gene="xylF"
                     /locus_tag="ACHKIR_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO9989731.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTTLNNGLKDVPSRLLTPIDVNKNNIKDTVVKDGFHKESEL"
     gene            13513..14835
                     /gene="xylA"
                     /locus_tag="ACHKIR_001987"
     CDS             13513..14835
                     /gene="xylA"
                     /locus_tag="ACHKIR_001987"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HFO9989732.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAGKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
     gene            14907..16361
                     /gene="xylB"
                     /locus_tag="ACHKIR_001988"
     CDS             14907..16361
                     /gene="xylB"
                     /locus_tag="ACHKIR_001988"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFO9989733.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDDMLQACHLSRDQMPALYEGSEITGALLPEVAK
                     AWSMAAVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLSNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            16530..16871
                     /gene="yiaB"
                     /locus_tag="ACHKIR_001989"
     CDS             16530..16871
                     /gene="yiaB"
                     /locus_tag="ACHKIR_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaB"
                     /protein_id="HFO9989734.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGILMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSA"
     gene            16916..17353
                     /gene="yiaA"
                     /locus_tag="ACHKIR_001990"
     CDS             16916..17353
                     /gene="yiaA"
                     /locus_tag="ACHKIR_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaA"
                     /protein_id="HFO9989735.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(17395..18390)
                     /gene="wecH"
                     /locus_tag="ACHKIR_001991"
     CDS             complement(17395..18390)
                     /gene="wecH"
                     /locus_tag="ACHKIR_001991"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HFO9989736.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLLFYSAIALLYIALFTSINV
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVCAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAITLLTLVKNTLDTRTVRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            18564..18872
                     /gene="ysaB"
                     /locus_tag="ACHKIR_001992"
     CDS             18564..18872
                     /gene="ysaB"
                     /locus_tag="ACHKIR_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HFO9989737.1"
                     /translation="MMMNSFFPAMALMVLMALVGCSTPPPVQKAQRVKVDPLRSLNME
                     ALCKDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR
                     "
     gene            18967..19878
                     /gene="glyQ"
                     /locus_tag="ACHKIR_001993"
     CDS             18967..19878
                     /gene="glyQ"
                     /locus_tag="ACHKIR_001993"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFO9989738.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            19888..21957
                     /gene="glyS"
                     /locus_tag="ACHKIR_001994"
     CDS             19888..21957
                     /gene="glyS"
                     /locus_tag="ACHKIR_001994"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFO9989739.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPSEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNTNVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLDAAASLAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRINRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            22279..22431
                     /gene="hokA"
                     /locus_tag="ACHKIR_001995"
     CDS             22279..22431
                     /gene="hokA"
                     /locus_tag="ACHKIR_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012540334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HFO9989740.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMVRDSLCELHIKQGSYELAAFL
                     ACNLKE"
     gene            complement(22619..22831)
                     /gene="cspA"
                     /locus_tag="ACHKIR_001996"
     CDS             complement(22619..22831)
                     /gene="cspA"
                     /locus_tag="ACHKIR_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HFO9989741.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(23112..23402)
                     /gene="yiaG"
                     /locus_tag="ACHKIR_001997"
     CDS             complement(23112..23402)
                     /gene="yiaG"
                     /locus_tag="ACHKIR_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HFO9989742.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            23836..24546
                     /gene="yiaF"
                     /locus_tag="ACHKIR_001998"
     CDS             23836..24546
                     /gene="yiaF"
                     /locus_tag="ACHKIR_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HFO9989743.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(24596..25570)
                     /gene="ghrB"
                     /locus_tag="ACHKIR_001999"
     CDS             complement(24596..25570)
                     /gene="ghrB"
                     /locus_tag="ACHKIR_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HFO9989744.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTIEQNAAILA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(25674..26333)
                     /gene="yiaD"
                     /locus_tag="ACHKIR_002000"
     CDS             complement(25674..26333)
                     /gene="yiaD"
                     /locus_tag="ACHKIR_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HFO9989745.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            26486..28819
                     /gene="bisC"
                     /locus_tag="ACHKIR_002001"
     CDS             26486..28819
                     /gene="bisC"
                     /locus_tag="ACHKIR_002001"
                     /EC_number="1.8.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HFO9989746.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALELIHHQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVMVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETIDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKNAEWAAEICGVGAAKIRELAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMLVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRAAVLSSMQGSLPGGTDAVDK
                     IPVARIVEALENPGGAYQHNGMDRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSELQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFRRDPQAHPLKTASGKIEIFSQRIADYAYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGIQD
                     GDTVRLWNARGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(28788..29228)
                     /gene="yiaC"
                     /locus_tag="ACHKIR_002002"
     CDS             complement(28788..29228)
                     /gene="yiaC"
                     /locus_tag="ACHKIR_002002"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFO9989747.1"
                     /translation="MIREVQHSELPAILELWLESTTWGHPFIKSSYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRHPHLML
                     EVYQKNQPAIDFYQAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(29225..29788)
                     /gene="tag"
                     /locus_tag="ACHKIR_002003"
     CDS             complement(29225..29788)
                     /gene="tag"
                     /locus_tag="ACHKIR_002003"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFO9989748.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRASFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTPASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCHPGNKP"
     gene            29946..30644
                     /gene="yhjY"
                     /locus_tag="ACHKIR_002004"
     CDS             29946..30644
                     /gene="yhjY"
                     /locus_tag="ACHKIR_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFO9989749.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLADPLWHASVSTLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALSQAENTTN
                     NSDYLYTMGVSARF"
     gene            30873..32081
                     /gene="yhjX"
                     /locus_tag="ACHKIR_002005"
     CDS             30873..32081
                     /gene="yhjX"
                     /locus_tag="ACHKIR_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9989750.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTMASGILLGLGFFLTAHSDNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDAISAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSICGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            32405..34096
                     /gene="eptB"
                     /locus_tag="ACHKIR_002006"
     CDS             32405..34096
                     /gene="eptB"
                     /locus_tag="ACHKIR_002006"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HFO9989751.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GISAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPVNAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQAREAAAN"
     gene            34188..34264
                     /locus_tag="ACHKIR_002007"
     tRNA            34188..34264
                     /locus_tag="ACHKIR_002007"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:34222..34224,aa:Pro,seq:cgg)
     gene            35009..36616
                     /gene="dppA"
                     /locus_tag="ACHKIR_002008"
     CDS             35009..36616
                     /gene="dppA"
                     /locus_tag="ACHKIR_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HFO9989752.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPKIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            complement(36679..36906)
                     /locus_tag="ACHKIR_002009"
     CDS             complement(36679..36906)
                     /locus_tag="ACHKIR_002009"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9989753.1"
                     /translation="MTRSLELRVCIAHKSIPALVEQGMKYCCRMALHAEAQRHRVCRL
                     LTKSALFMPDAAQTPYPTYKIVQTQYIAEFS"
     gene            36924..37943
                     /gene="dppB"
                     /locus_tag="ACHKIR_002010"
     CDS             36924..37943
                     /gene="dppB"
                     /locus_tag="ACHKIR_002010"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HFO9989754.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRLRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            37953..38855
                     /gene="dppC"
                     /locus_tag="ACHKIR_002011"
     CDS             37953..38855
                     /gene="dppC"
                     /locus_tag="ACHKIR_002011"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HFO9989755.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVVIVLF
                     IAIFANWIAPYNPAEQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            38866..39849
                     /gene="dppD"
                     /locus_tag="ACHKIR_002012"
     CDS             38866..39849
                     /gene="dppD"
                     /locus_tag="ACHKIR_002012"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HFO9989756.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            39846..40850
                     /gene="dppF"
                     /locus_tag="ACHKIR_002013"
     CDS             39846..40850
                     /gene="dppF"
                     /locus_tag="ACHKIR_002013"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding subunit
                     DppF"
                     /protein_id="HFO9989757.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(40880..42151)
                     /gene="yhjV"
                     /locus_tag="ACHKIR_002014"
     CDS             complement(40880..42151)
                     /gene="yhjV"
                     /locus_tag="ACHKIR_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HFO9989758.1"
                     /translation="MQHNTLPKHDQKMPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYVTLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            42627..42734
                     /gene="ldrD"
                     /locus_tag="ACHKIR_002015"
     CDS             42627..42734
                     /gene="ldrD"
                     /locus_tag="ACHKIR_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO9989759.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASLIVNWLNKRK"
BASE COUNT        10507 a        10558 c        11151 g        10847 t
ORIGIN      
        1 cgagattaat cgccagtatt gcttcaagta tttttttcat agctattcct tgaggctatg
       61 tctgtcataa ttcaagttgc atgtgcagca acctaaatta ttaatatagt tagagcaata
      121 acattaccca ggcgttctgg cgtagacgat tattcgatta attcagcgct gttaatgcga
      181 ctttaaccac aatttttcgt atctcagagg ttgtttgcag aatacaacct ggtcggtgaa
      241 catcctgcgg gaagaaaata gcgtagctgc ccggtatcat ttctataaat gattcatgtt
      301 cactatcgtg ataaaaaata atatcgcgct gctctaatag tgattcgctg actttattat
      361 ttcccgtatc aatagcaatg ccgattttct cttcacccca cgccagaaac tgaatatcga
      421 tataccgacg atgcacttcc ggacggtttt ccgccgcttc gcgtgtggtt aaatcgataa
      481 tttgcgcata aatattcttg ccgtcgattt cgacaacgcc cggttccagg gcgttgaagt
      541 cggtggtgcg cagaaaatca agcgcctttt caatggcggc aggcaaacgg cacgggtcag
      601 gctgcgcgat atgtccaaaa atcatgggtt atctccttat aacgcttgga tttttgccca
      661 cacgctgtca tcaacggtaa taccgttacg gcggttttcg gccagcaggg tagtaaattc
      721 gtggccgggt aagcgaatgg cctgattttc gtcagcgcgt tcagcggtgg taacgtaatc
      781 catgatgcgt tgcagtttgg catcgcgggt ggggccgtcg ataagcttat ctacttcaat
      841 ggcaataaaa atttgtgaaa tgccgtattc gtcgctgttg tcctgggtga cttcggcaac
      901 cgatgcgccg tcagaaagga gagtggcgat catatccagc acaatcgaca tgcccgaacc
      961 tttccagtaa cccatcggca aaatgcggcg attcttctcg ataacgccag gttctttggt
     1021 caaattgccc tcatcatcaa agccaccatc gaccgggagc tgacggcctg ccagacggtt
     1081 aacttctaac atgccgtaag agaacatcga catcgacata tcgaccatgg tgattggggt
     1141 agaaggaatg gcgacgatca gcgggttagt gccgatgcga cactcttttg cgccccacgg
     1201 cggcattacg gcgatggagt tggtccagca aatgccaata tagccttttt ccgccgcctg
     1261 ccagccgtag ctgccgccgc gcatccagtg gttggcatta cgtagtgcca ccagaccaat
     1321 accgtgatcg gcagccagtt caatggcgcg atccatcatc tttttcgctg tcaggttacc
     1381 gatcgaacgc tgggcgtccc actgttcaat tgcgccgagg ctggttatac gtttgggttg
     1441 ggcatcagga atgatatcgc cgttttccag ttgttgaatg aaacgaggga aacggttgac
     1501 gccgtgagaa taaacgccgg attcggtggt gcgggcgaac atttcggcac aggcatcagc
     1561 cgtttcgctg tcaacgtcgc gtgaaattaa gactcgatta aaggctgctt ttaactgctc
     1621 aaatgtcact ttcatccctg gcttccttgt ttttttgatc gcttttttgg cttaaaattt
     1681 caatatgcga aatctgattt caaatatatc gatcgctttt taacaaagca atctgattga
     1741 tgaatttcaa aaaacttaaa aatcaagtgg ttataaatta tctgtcgaga tcgtgaacta
     1801 cgacacactt tgcgctacca tcttgacgcg acaaaatggg gaaagaagtg atgggaaaaa
     1861 aagagaacga gatggcgcag gaaaaagagc gtccagccgg aagccagagt ctgtttcgcg
     1921 ggttgatgct gattgagatt ttgagcaact atccaaacgg ttgtccgttg gcgcatcttt
     1981 cggagctggc tggtttaaat aagagtaccg tccatcgctt attgcaggga ttacaatcct
     2041 gcggctatgt gaccaccgcg cccgccgcag ggagttatcg cctgaccacc aaatttattg
     2101 ccgtcgggca gaaggcgctg tcttcgctga atatcattca tatcgccgct ccgcatcttg
     2161 aggcactgaa catcgccact ggtgaaacta ttaacttctc cagccgcgaa gacgatcacg
     2221 ctattttgat ttataagctg gaacccacaa ccgggatgct gcgaacccgt gcctatattg
     2281 gccagcatat gccgctctac tgttccgcaa tgggcaagat ctacatggcg tttggccacc
     2341 cggactacgt gaagtcatac tgggaaagcc atcagcatga gatccagccg ttaacccgca
     2401 ataccattac cgagctgccc gcgatgttcg acgaactggc gcacattcgt gaaagcggag
     2461 cggcgatgga cagagaagaa aacgaactcg gcgtctcctg tattgctgtt ccggtgtttg
     2521 atattcatgg gcgggtgccg tacgccgtgt cgatttcgct ttcgacatca cgtctgaaac
     2581 aggtggggga gaaaaatctc ctgaaaccac tgcgtgaaac cgcgcaggct atttctaatg
     2641 aactgggatt tactgtcagg gacgaccagg gcgcgatcac ataacgcttt ggacaagtgc
     2701 caaaacttta acatttcctt cgttggatca aagcagtaga gacgcgctct ctggcactct
     2761 gctgttttag tgcaaaggag tgatcatgaa ccggtttatt attgcggatg cgacgaaatg
     2821 tatcggttgc cgtacctgtg aagtggcttg cgcagtgtcg catcaggaga atcaggattg
     2881 cgctgcgttg tcaccagacg agtttatttc ccgtattcgt gtcattaaag accacacatg
     2941 gaccacggct gtagcctgcc atcagtgtga agatgcaccg tgcgcgaatg tctgccctgt
     3001 tgacgcgata agccgcgaac atgggcatat tttcgttgaa caatcacgtt gcattggctg
     3061 taaaagctgt atgctggctt gcccgtttgg tgcgatggag gtcgtttctt cgcgcaaaaa
     3121 ggcgagggcg attaagtgcg atctgtgctg gcatcgggcg acgggaccgg cctgtgttga
     3181 agcctgcccg acaaaggcgt tgcagtgcat ggatgtcgag aaagtgcagc gacatcggct
     3241 acggcagcag cctgtttgaa acgtttttcc ggcgtttctc aacgcaggga tagtgcgctt
     3301 tgtttatgcc ggatgcggct aaaacgcctt atccggccta caaattctac caaattcaat
     3361 atattgcaga aatcatgtag gcctgataag cgtagcgcat caggcaattt ttagtgactt
     3421 tcagcccagg ctctttctat ctcttctgcc agaatcttca cacccgcctc aattttctcc
     3481 ggctccggca cgtagttcat gcgcatacat tgatgtgtat gtggccacgg tttatccagc
     3541 cctgggaaga agttgtgccc cggcaccatc agcacgcccc gtgctttcag gcgctggtag
     3601 agctgctcgg tcgtaatggg caaatcctta aaccatagcc agaggaaaat ggctccttcc
     3661 ggtttatgaa tcaggcagcg atcttccggt aaatagcggc gaatgatagc gatagtttcc
     3721 tgaacacgct ggtagtaaaa cggtttgatg actgtttcag acaggcgcag cagatcgtta
     3781 cgcttaatca tttcactcat catcgccgga ccaataccgc caggtgccag gctgataatg
     3841 ccgttcatat tggtgatggc ggtgatgatt ttttcattgg cgatgataat gccgcagcgg
     3901 gagcctggta gacccagctt ggaaagactc atgcacagta cgatattcgg attccacagc
     3961 gggcgcgctt cgctgaagat gatacccggg aacgggacgc cataagcgtt atcaatcacc
     4021 agcggaatgc cgtgttgatt cgccagcgcg tcaagcttca gcaactcttc gtcagtaatc
     4081 acattgcctg ttgggttcgt cggccgggag acgcaaatca tcccggtttc ttcgccaata
     4141 tgcagatgct caaaatcgac gtggtattta aactggcctt ccggcagcag ctcaatattc
     4201 ggacgcgcag agacaaacag atcttcttcc agtccggcgt cagcgtagcc aatgtattcc
     4261 ggtgccagcg gaaacagcac ttttttgatc cgaccatcgg cacggcgtcc ggcgaacaga
     4321 ttaaataagt agaaaaacgc gctctggctg ccgtttgtta gtgcaatatt ctgtggttcg
     4381 atatcccaac ccaacttctc gcgtagcatt ccggcaagca gtgtgagtag ctccgttttc
     4441 ccctgtggac cgtcgtaatt gcacagtgca tcagtcgctt tgccactttc cagcatgtcg
     4501 gtcagtagcg tctggaagta gtcctgcatt tccgggatct gcgctggatt accgccgccg
     4561 agcataatcg cgccaggcgt gcgtaaaccg tcgttcagat cttccatcag cagcgtaatg
     4621 ccggagtggc gggtaaattt gtcaccaaaa agggagaatg tcatagcggg atatctgtcg
     4681 aactctgtag caaggaaggt aacaataacg ctacatttgc tggggtgcaa atcgctctgt
     4741 tgaagaggga gcggtgcttt atcctgcaac gctgattagg tctgacattt tatcctggtt
     4801 ggcggcttcc cgaggtactg acgaacggga agccggaaaa gttactgttg cccagcccag
     4861 atgaccagca ctttatcgtc gccatgctca cgaacaaagc cgtagccctg cttcagcgaa
     4921 agtgtcgttt gtttgcccgc gccaattgcg ggatggcggg cgcggaactg gctgattttc
     4981 tgccagtgcg cgacgctggc ggcagatttg ccgctaacat cctgccagtt catatccgaa
     5041 cgcgtgcctt gcagtggatc cgaaccggtg gggccgaacg gacgcgagga ttcatcacca
     5101 taaaagattt gtaccgcgcc tggcgctaat aataactctg ctgctttgtc gcccccttca
     5161 cggaacaggc gggtatcatg cgacgagagg tagctcaaca cgttgaaatc ctgcaatttc
     5221 tccgccattt gctgccaggt cgtatccatc tgcgccagac aatcgactgc tttagccgcc
     5281 tgctcctgat aatcgaaatt gatcatcgca tcgaagccgt ggcgatagta gtcactttgc
     5341 atcacgccgt ggccccaggc ttcaccggtc atccagaacg gtttgtcatc taacgcttta
     5401 tcgggattcg cttttttcca ttcgacaagc gcggcgctgg cttcggtttt cagttgctgc
     5461 caggcgggca actcaacatg tttggcggtg tcgacccgaa aaccatcaat cccatagtcg
     5521 cgaacccact gacttaacca gtgggttaag taatcgcgag gggtaaagcc gtcgatgact
     5581 ttagcgtggg tatccgtttt gtttttatag aacaccggca gaccagaagc ggtcgttgat
     5641 tcggttttga tatccggcaa aaaggccagc gacatagtga gatcgtcaaa tccaggattg
     5701 tcgtaatcgc cgatatcggt tctgatccag ttttttcccc accatttatc ccagcctgtt
     5761 ttgtcgctga aattaatgta atcgttaaag ctatgccagg tttgcccggc ggcaggtttc
     5821 cagtcgctcc agcgttcacc caacgttttt ttcacttcgt caccagaaag atataacgcg
     5881 ccaaactgat actcctgcat atccgccagt gtggcatagc cggtgtggtt catcacgaca
     5941 tcaaagagaa tacgaatacc gcgctgatgc gcgctatcaa ccagtgtccg tagatcggct
     6001 tcactgccca tattggcatc aagattcgtc cagtcctgtg tgtaataacc gtggtaggca
     6061 taatgcggga aatcgccttt tgtaccgccg ccgacccagc cgtgaatttg ctcaaatggg
     6121 gcacttatcc ataaagcatt gacgcccagc tgctggaggt aatccagttt gttggtcagg
     6181 ccgcgtaaat cgccgccgtg aaaagtgcca atttctgcca taccgtcttt atgacgtccg
     6241 taactctggt cattactggg atcgccgttt tcgaaacgat ctgtcagcac aaagtaaacc
     6301 gtggcgttat gccagtcgaa aggggcaggg gcgtcagttt ctgcccgttc cagcaggagt
     6361 aaaccgttgc tggtggcagc gggttgtaac gttatttgac cgttcttcac tatcgcaatt
     6421 tgctggctgt aataatcccg gacggcggct ccctccggaa aagtggcgct gacatccagt
     6481 gtgagcggta atccatccca tttcgggcat tcacggacca ggcttgctac cggttcgacg
     6541 gcgttctgga tggaaatcat caatgttggt gtaccggagc gggtgtctag ttgcagcgta
     6601 tattcgccgt ccctgaacaa tcgccattga ggcggcgtgt tgctacaagg ttgcagggaa
     6661 agcatctgat tgagttttat cgcatccgca ggctgccagc actgttggtc aaaatttagc
     6721 gttagtggac gcgtaccttt gggcaactgc gcgtggctga caaatgttcc cgtcccctgt
     6781 tcgctaaagg tgggaaaccc cggagaagtc cagctggcgg caacggcgaa gccaggaagg
     6841 agtgtcagaa aacaggcggc gagtttcatg gcggatgagt ccttattgct gcgatttcag
     6901 acagtttgcc agcgataagc cagacaaaac tcatccctga gcggtaagtc cacaggatga
     6961 gaagcaaggg tgagcgatcg cgcacaaaaa acggctgaat tttgcgataa accccacatt
     7021 ttctgcgatt tagcgccaat ctgaatcgtt aactcgtgac atagtttcag atttggacta
     7081 ttccttgggg tgctcttgac agctattttt gatatgattt gagattcaac tctcaaattt
     7141 gtgaaaaata taaggtgttg gaatgattaa atccgaccag gagacctgat gattttgact
     7201 cccatacgac gatatggggc gatgattctt atgttactca ctctggtgtt ttcgagtgag
     7261 gtgttagcga agacgcacac aacaacagcg agtcaaaagt cccacttaac taaagctagt
     7321 aataaacagg taagcagtaa acaagagtat tctcgcaata gtgcaaagag tagttcactt
     7381 cctgatttgc gaaaataccc ttccggaaca ccaaggaaaa aggcgtttct ccggactgta
     7441 atgccttaca ttaccagcca aaatgcggcc attactgcgg aacgtaactg gctgatttca
     7501 aaacagtatc agggccaatg gtcacctgct gagcgtgcgc gtctgaaaga catcgccaaa
     7561 cgctacaagg tgaagtggtc tggtaatacg cgaaaaatcc cgtggaatac cttgcttgaa
     7621 cgcgtagaca ttatccctac cagtatggtg gcgacgatgg ctgcagcaga aagcggttgg
     7681 ggaacgtcaa agctggcgcg caacaacaac aatctgttcg gcatgaaatg catgaaagga
     7741 cgttgtacca atgcgccggg taaagtgaaa gggtactcac agtttagttc tgtcaaagaa
     7801 tcggtgagcg cctatgtcac taacctgaat actcatccgg cttactcttc gttccgtaaa
     7861 tcgcgtgcac agctgcgtaa agcggatcag gaagtgactg ccacagcgat gattcacaag
     7921 ctgaagggct actcgactaa agggaagagt tataacaact acctgttcgc aatgtaccag
     7981 gataaccaac ggttaatcgc ggcgcatatg taattgcatt tcctcgagcc ttatccgact
     8041 tgtcagtcgg ataaggcttt tgcttgcatc aggtggttgt gctgagttac ctgatgcgac
     8101 gccgacaatt ctcatcatcg ctacaacatg acctcgctat ttacatcgcg atactctttt
     8161 ggcgtcgtgt catatgcttt tttaaaaaca gagtagaaat attgcagcga tggataaccg
     8221 cacatttgcg atatctcatt gatcgacaag gtggttgaaa tcagcagact gcgcgctttc
     8281 tccagcttct cggcatgaat catggcatgg atggtttcac ccacctcttc tttaaaacgc
     8341 ttctcaagat tggagcgcga gatcccgacc gcatccagta cctgatccac tttaatccct
     8401 ttacaggcgt gattacgaat gtaatgcatg gcctgaataa cggcgggatc ggtcagcgag
     8461 cggtaatctg ttgagcgccg ttcaatgacg cgaactggcg ggaccaaaat ccgctgtagc
     8521 ggcatttctt ctttatctaa taatcgatgc aacagttttg ccgcctgata gcccatttgc
     8581 cgcgcgccct gagcgaccga agaaagggcg acacgcgaca gatagcgggt cagttcttcg
     8641 ttatcgatgc caatcacgca taatttttcc ggtacgggaa tatgtagatg ttcacatact
     8701 tgcagaatat gccgcgcccg ggcgtcagta acggcaataa tcccggtttg cggtggcagc
     8761 gtttgtagcc agtctgccag ccgattttgc gcgtgttgcc agttctctgg cgcggtttct
     8821 aacccctgat aaaccactcc gcgatacttt tcttcggcga caagctgacg aaatgcatat
     8881 tcgcgttcag tggcccaacg tttaccgctt gattccggaa gaccataaaa agcaaagcgg
     8941 ttaacgcctt tctcttttaa atgcaaaaat gcgctttcaa ccagcgcata gttatcggtg
     9001 gcaatgtaat gaacgggtgg gtaactttcg gcaaggtgat acgagccacc aaccccaaca
     9061 atggggacgt cgacatcagc cagcgcttgc tcgatttgtt tgtcgtcgaa gtcggcaatg
     9121 acgccatctc ctaaccagtc cttgatttta tcaatgcggg cgcggaaatc ttcttcaatg
     9181 aaaatatccc attccgattg tgacgcctgt aaatattccc ctacgccttc tactacctgc
     9241 cggtcatagg ctttattggc attgaacagt aatgtgatgc ggtgacgttt agtaaacatg
     9301 gttcttttcc tgctgaatca tgcaaaaact caaaaccggt aatacgttac cggctttgag
     9361 aaaattttta tcaaaatcaa gaacggcgtt tggttgcgga gtccatccat acagccagca
     9421 acagaatcgc acctttaacg atatactgcc agaaggtcgg tacatccatc atactcatgc
     9481 cgttatccag tgaagccatg atgaatgccc ccattaccgc tccagccacg ctcccgacgc
     9541 cgcctgccag actggtgccg ccaatcacac atgctgcaat tgcgtccagc tcggcgatat
     9601 tccccgcaga aggtgaacca gcgccaagtc gagaactgag gattaatccg gcgatggcta
     9661 ccattaatcc gttaatcgcg aacacggcaa gtttggtgcg ttcaacgtta atcccggaga
     9721 gacgtgccgc ttccagattg ccgccgatgg cataaatgcg tcgtccaaat gctgtccgcg
     9781 ttgccataaa cattccgccg agtaacagca acgtcagcag cagaacagga gtgggaacgc
     9841 cacggtaatc attcaacagc cagattgcgc ctaatacgat gatagcggtt aaagcctggc
     9901 gaccaactac tgcggtagag gcgggagatt gcaaacccaa agcctgacgg cgcattcttc
     9961 cgcgccattg ccagccaaca aaagccatta agccaagcgc gccaatgatg aagccagtgc
    10021 tggcaggtag atagctttgc ccaatttgtg acatcgcggc gctggtgggg gatacggtcg
    10081 tgccgttggt gatgccaatg agtatgccgc gaaatgccaa catgcccgcg agggtgacaa
    10141 taaatgaagg gactttgcgg tacgcgaccc accatccgtt ccaggcaccg agaagcaatc
    10201 ccagaaccag cgttacaatg atggtaagtg gcaaaggcca gcctagccag acgtcacaaa
    10261 tcgccgcgac gccacctaac agccccatca ttgagccgac ggaaaggtcg atttcagcag
    10321 aaattatgac gaacaccatt cctaccgcga ggatgccggt aatcgcggtt tggcgtaaca
    10381 ggttggagac gttacgggcg cttaagtagg caccatcggt ggtccaggta aagaacagca
    10441 tgattgcgat gatagctgca atcatcacga agacctgcaa attcagtgat ttcagcccgg
    10501 aaaagccacc ggatgtcggt acggctaatt tcacttcaga cggattgctt ttcgacatga
    10561 tgttcgctcc tcaatgcggc ttccatcacc tgctcctgag tcaggttatg atttatcagg
    10621 ttggctttta gtttcccttc atgcatcacc agtacacgat cgctaaggcc gagcacttca
    10681 ggtaattcgg aagagatgac aataacggca ataccctgct ggacgagttg gttaattaat
    10741 ttgtagatct cgtatttcgc gccaatatcg atacccctgg tgggttcatc aagaatgaga
    10801 atgcgcgggt taagtaacag acagcgagcg aggatcgctt tttgctgatt gccgccgctc
    10861 aaacgtccaa tagcaaggtc gggggacgac gttttaactt tgagttgctg gattgattcc
    10921 agaatacatt tttgctctgc cgcgtcatca agctggctaa tgccaccggt aaattttttg
    10981 agtgcggcga gggtaatatt tttaccaacc gccattaccg gaacgatgcc gtcgcgcttt
    11041 ctgtcttcgg gtaccatcgc aatcccctgg gcgatggctt gctgacagtt acgaatatct
    11101 acctgtttgc catcaatata aattttccct tcccattgtc cgggccacac accaaacagg
    11161 cactgaatag tctcggtacg tccggcacca acgagtccgg cgatacccag tatttcgcca
    11221 cgtttcaggg aaaacgagac atcattaact cgtttaatat gacgattaac cggatgccat
    11281 gccgtcagat gttcaatacg taatatttca tctccggtgg tatgtggttc attagggtaa
    11341 agcgcggtta actctcgtcc gaccatcatg gtgataatat cgtcttcact cattccagca
    11401 gcatcacgcg tgccaatgtg ctgaccgtcg cgaataacgc aaatcgtatc ggaaatcgct
    11461 ttgacttcgt tgagtttgtg cgaaatataa atacaggcga taccgtgctg ttgtagatcg
    11521 cgaataatat ccagtaaaac cgacgtttcc tgctcagtta atgaggctgt cggctcatcg
    11581 agaattaaca aacgcacctg tttattaagt gccttggcaa tttcaaccag ttgttgttgc
    11641 ccaagtccta aatcgccaac gcgggtatca ggtgaaatgg ataaactgac ctgtgcgagc
    11701 agcttctgac agcgtagcgt catcagatca taatccataa tgccattatg ggttatttcg
    11761 ttacccagga agatattttc cagcacggtc aattctttca ccagggccaa ttcctggtga
    11821 atgatggcga tacctttgcg ttcggtatcg cggatgtgac tcgcctgaat ctcttctccc
    11881 gcaaaaataa tttcgccttc gtaggaacca tggggataaa taccacacag cactttcatc
    11941 agcgttgatt taccagaccc attttcccca cagagtgaga cgatttcgcc agcattcaac
    12001 cgcaagctga cgttatcaat cgccttcaca ctgccgaagg ttttggtaat gttcttcatt
    12061 tcaagtagat aaggcataac gactccacct aagccaatta attcacacgg catggagaga
    12121 aatcacgccc ccgctcctcg ccggggcgta acgcttacag ctcgctctct ttgtggaatc
    12181 cgtctttaac taccgtgtct ttgatgttgt ttttattcac atcgatcggt gtcagcaggc
    12241 gggagggaac atctttcagg ccattattca gtgtggtatc tgcttttggt tcctgaccat
    12301 tgcccaactc aacggcaatt tctgcggcag tatttgccaa caacgtaata ggtttataca
    12361 ccgtcatagt ttgcgtaccg gcagcaatac gtttaatacc tgcgagatcc gcatcctggc
    12421 cggaaattgc tactttccct gataaacctt gcgcgcttaa tgcctgaatt gccccacctg
    12481 cggtggcatc gtttgaggca actacagcat caattttgtt attattggcg gttagcgcgt
    12541 tttccataat tttcaatgcg ttttccggta accagccatc aacccattgg tcaccaacga
    12601 ctttaatttt tccagaatca acgtaaggtt ttaacacttt catttgtccg gcgcggaaca
    12661 gcttagcgtt gttatctacc ggcgagccgc ccatcaggaa gtaattacct tgcggaacaa
    12721 tatcgaccag ggcttttgcc tgcagttcac cgactttttc gttatcgaaa gaaatataaa
    12781 aatcgatatc cgcatcgtta atcatacggt cgtaagctaa tactttaata ccttcttgtt
    12841 tggcttcttt tacaacgtta cttaatacct gaccgttata aggaataatg acaagaacat
    12901 cgacaccccg gttaatcatg ttttcaatct gcgacatttg tgtttcttca ttgccatttg
    12961 cagactgtac aaatactttc gcgccgagag attctgcttt tttcacaaaa atatctcgat
    13021 ctttttgcca gcgttcaaga cggagatcat caatcgccat acctattttg acttctttgg
    13081 cgtgtgccgc aacgttggta agcagaagtg aggtgcaaag ggtgagtaga atgttcttta
    13141 ttttcatggt gtagggcctt ctgtagttag aggacagctt taataagtaa caatcaccgc
    13201 gataaacgta accaattttt agcaactaaa caggggaaaa caattacaga tttttatctt
    13261 tcgattacga tttttggttt atttcttgat ttatgaccga gatcttactt ttgttgcgca
    13321 attgtactta ttgcattttt ctcttcgagg aattacccag tttcatcatc ccattttatt
    13381 ttgcgagcga gcgcacactt gtgaattatc tcaatagcag tgtgaaataa cataattgag
    13441 caactgaaag ggagtgccca atattacgac atcatccatc acccgcggca ttacctgatt
    13501 atggagttca atatgcaagc ctattttgac cagctcgatc gcgttcgtta tgaaggctca
    13561 aaatcctcaa acccgttagc attccgtcac tacaatcccg acgaactggt gttgggcaag
    13621 cgtatggaag agcacttgcg ttttgccgcc tgctactggc acaccttctg ctggaacggg
    13681 gcggatatgt ttggtgtggg agcgtttaat cgtccgtggc agcagcctgg tgaggcactg
    13741 gcgttggcga agcgtaaagc agatgtcgca tttgagtttt tccacaagtt acatgtgcca
    13801 ttttattgct tccacgatgt ggatgtttcc cctgagggcg cgtcgttaaa agagtacatc
    13861 aataattttg cgcaaatggt tgatgtcctg gcaggcaagc aagaagagag cggcgtgaag
    13921 ctgctgtggg gaactgctaa ctgctttaca aaccctcgct atggcgcggg tgcggcgacg
    13981 aacccagatc ctgaagtctt cagttgggcg gcaacgcaag ttgttacagc gatggaagca
    14041 acccataaat tgggcggtga aaactatgtc ctgtggggtg gtcgtgaagg ttacgaaacg
    14101 ctgttaaata ccgacttgcg ccaggagcgt gaacagctgg gccgctttat gcagatggtg
    14161 gttgagcata aacataaaat cggtttccag ggcacgttgc ttatcgaacc gaaaccgcaa
    14221 gaaccgacca aacatcaata tgattacgat gctgcgacgg tctatggctt cctgaaacag
    14281 tttggtctgg aaaaagagat taaactgaat attgaagcta accacgcgac gctggcaggt
    14341 cactctttcc atcatgaaat agccaccgcc attgcgcttg gcctgttcgg ttctgtcgac
    14401 gccaaccgtg gcgatgcaca actgggctgg gacaccgacc agttcccgaa cagtgtggaa
    14461 gagaatgcgc tggtgatgta tgaaattctc aaagcaggcg gtttcaccac tggcggtctg
    14521 aacttcgatg ccaaagtacg tcgtcaaagt actgataaat atgatctgtt ttacggtcat
    14581 atcggcgcga tggatacgat ggcgttggcg ctgaaaattg cagcacgcat gattgaagat
    14641 ggcgagctgg ataaacgcat cgcgcagcgt tattccggct ggaatagcga attaggccag
    14701 caaatcctga aaggccaaat gtcactggca gatttagcca aatatgctca ggaacacaat
    14761 ttgtctccgg tgcatcagag tggtcgccag gagcaactgg aaaatctggt aaatcattat
    14821 ctgttcgaca aataacggct aactgtgcag tccgttggcc cggttatcgg tagcgatacc
    14881 gggcattttt ttaaggaacg atcgatatgt atatcgggat agatcttggc acctcgggcg
    14941 taaaagttat tttgctcaac gagcagggtg aggtggttgc ttcgcaaacg gaaaagctga
    15001 ccgtttcgcg cccgcatcca ctctggtcgg aacaagaccc ggaacagtgg tggcaggcaa
    15061 ctgaccgcgc aatgaaagct ctgggcgatc agcattctct gcaggacgtt aaagcattgg
    15121 gtattgccgg ccagatgcac ggtgcaacct tactggatgc ccagcaacgg gtattacgcc
    15181 ctgccatttt gtggaacgac gggcgctgtg cgcaagagtg cactttgctg gaagcgcgag
    15241 ttccgcaatc acgggtgatt accggtaacc tgatgatgcc cggatttact gcgcctaaat
    15301 tgctatgggt tcagcggcat gagccggaga tattccgtca aatcgacaaa gtattattac
    15361 cgaaagatta cttgcgtctg cgtatgacgg gggagtttgc cagcgatatg tctgacgcag
    15421 ctggcaccat gtggctggat gtcgcaaagc gtgactggag tgacgacatg ctgcaggctt
    15481 gccacttatc tcgtgaccag atgcccgcat tatacgaagg cagcgaaatt actggtgctt
    15541 tgctacctga agttgcgaaa gcgtggagta tggcggcggt gccagttgtc gcaggcggtg
    15601 gtgataatgc agctggtgcg gttggtgtag gaatggttga tgctaatcag gcaatgttat
    15661 cgctggggac gtcgggggtc tattttgctg tcagcgaagg gtttttaagc aagccagaaa
    15721 gcgccgtaca cagcttttgc catgcgctac cgcagcgttg gcatttaatg tctgtaatgc
    15781 tgagtgcagc gtcgtgtctg gattgggccg cgaaattaac cggcctgagc aatgtcccag
    15841 ctttaatcgc tgcagctcaa caggctgatg aaagtgccga gccagtttgg tttctgcctt
    15901 atctttccgg cgagcgtacg ccacacaata atccccaggc gaagggggtt ttctttggtt
    15961 tgactcatca acatggcccc aatgaactgg cgcgagcagt gctggaaggc gtgggttatg
    16021 cgctggcaga tggcatggat gtcgtgcatg cctgcggcat taaaccgcaa agtgttacgt
    16081 tgattggtgg cggggcgcgt agtgagtact ggcgtcaaat gctggcggat atcagcggtc
    16141 agcagctcga ttaccgtacg gggggggatg tggggccagc actgggcgca gcaaggctgg
    16201 cgcagattgc ggcgaatcca gagaaatcgc tcattgagtt gttgccgcag ttaccgttag
    16261 aacagtcgca tctaccagat gcgcagcgtt atgccgctta tcagccacga cgagaaacgt
    16321 tccgtcgcct ctatcagcaa cttctgccat taatggcgta aacgttatcc tctgcctgac
    16381 cgggtggggg ataattcaca tctatatatc tcagtaatta attaatattt agtatgaatt
    16441 tattctgaaa atcatttgtt aatggcattt ttcagttttg tctttcgttg gttactcgta
    16501 atgtatcgct ggtagatatg gagatcgtta tgaaaacctc aaagactgtg gcaaaactat
    16561 tatttgttgt cggggcgctg gtttatctgg ttgggctatg gatctcatgc ccattgttaa
    16621 gtggaaaagg ctattttctt ggcatattaa tgacagcaac ttttggcaac tatgcatatc
    16681 ttcgcgcaga aaaactcggg caactggata atttttttac ccatatctgc cagttagttg
    16741 cgttaatcac tatcggtctc ttgtttatcg gtgttttaaa cgcacctatc aatgcttatg
    16801 aaatggtgat ctaccctatc gccttttttg tctgcttgtt tggtcaaatg cgtttgtttc
    16861 gctcggcatg agcaacataa agctcttaca cattcaggaa tgaaggaata ctgtgatgga
    16921 caacaaaata tcaacctatt ctccggcatt cagtattgtg tcatggatag ctctcgttgg
    16981 tggtatcgtt acctatctgt tagggctatg gaatgcagag atgcagttaa atgaaaaagg
    17041 atattatttt gccgtactgg tattaggact gttttctgcg gcgtcttatc aaaagaccgt
    17101 tcgggacaag tatgaaggca taccgaccac ctccatttat tatatgacct gcctgactgt
    17161 ctttattatc tctgttgcgt tactgatggt aggtctgtgg aatgcaacgt tattactcag
    17221 cgagaaaggt ttttatggac tggctttctt cttaagcttg tttggtgcag tagcggtgca
    17281 gaagaatatt cgtgatgccg gaataaaccc accaaaagaa acacaggtta cccaggaaga
    17341 atacagcgaa taattcacat aagcccggtc agtccaatgt gaccggcctt ttacttaact
    17401 cactagtctg tttctgtcga ttcgttgtac cagcatagaa agtaacaaac tcgctgccaa
    17461 cgtcgcgcaa aagatccaaa taatatcgag tattggccag tttttaagct caatcccccg
    17521 ggtgcgcagt gcatggataa tcaaggcgtg gaacccgtat atgcccaacg aatggcggga
    17581 gattaagcca agtccgcgaa cggtacgcgt atcaagcgtg tttttaacca gagtcaatag
    17641 cgtgattgcg cagataaaaa ccatcggccc acagtaaaga taccaggtat cggcaaaatt
    17701 tccgcgccac tgcaattcat ataatgtccc gcgagagata ataaaaaccc ccgtcgcaaa
    17761 cagtgctgcg cacacccacg acagtgcttt atgctgtgtg tccatcatcc ctatagcgcg
    17821 gcccaacatg ccatacagga tgtagtaaaa agtatcgcca ttgatatata agttaattgg
    17881 cagccattca aaaccgtcaa ttttctgcgg cactgtgttt gggttagcga taataccaat
    17941 caccaccatt agcaccagca acatttttcc gccgacgttc ttcacctgaa tcagcggtga
    18001 aaccagataa atcaccgcaa tcgcgaagaa aaaccataag tggtaaaaca ctggcttttg
    18061 cagcaggttt ttcagcgcta actccacatt gatggaggta aacagcgcaa tgtagagcag
    18121 tgcgattgcg ctataaaaaa gcagacataa gccgatacgc aagaaatggc gcggctgggc
    18181 gctgcgttcg ccaaaaaaga gatagccgga aatcatgaaa aatagcggca cgctgacacg
    18241 agaggcagaa ttcagaacat tggcgatatc ccaggtgacg gggctaacac tatgagcatt
    18301 ggtcacatac caggtagtgg tgtgaatcat caccaccatt aaacacgcta tccctcgcag
    18361 gttatcaatc cagtaaattt tgggctgcat ctgtgtctct gtatctggtt aaaaaagtct
    18421 gaccgataaa tcattcggtt ggcgcactgg aataatctga gttttatcac tacagcttat
    18481 agaggcttaa ggaaattcgt aagatatcag ccactatacc gatataaata ataagactca
    18541 cctgcaaacc agacggtaat ttaatgatga tgaactcttt ctttccggca atggcgctta
    18601 tggtgcttat ggcgctagtg ggttgttcta caccgccacc cgtgcagaaa gcacaacggg
    18661 taaaggttga tcctctgcgt tcgttgaata tggaagcgtt atgcaaggat caggcggcga
    18721 aacgttataa caccggcgag caaaaaatcg acgtcaccgc atttgaacag ttccagggaa
    18781 gctacgaaat gcgcggttat accttccgta aagagcagtt tgtctgttct tttgacgcag
    18841 atggccattt tttgcatctt tccatgcgtt aagccctgct ttttcccgtt tcgtactgta
    18901 tatcttccat ccagcgggta tactgatccc ttcctttaaa tccacacgta tccagcacga
    18961 aataatatgc aaaagtttga taccaggacc ttccagggct tgatcctgac cttacaggat
    19021 tactgggctc gccagggctg caccattgtt caaccattgg acatggaagt cggcgcggga
    19081 acctctcacc caatgacctg tctgcgcgcg ctggggccag aaccgatggc ggctgcttat
    19141 gttcagcctt ctcgtcgccc gaccgatggt cgctacggcg aaaaccccaa ccgtttacag
    19201 cactactatc agttccaggt ggtcattaag ccatcgccgg acaatattca ggagctgtac
    19261 cttggttctc tgaaagagct gggcatggac ccgaccatcc acgacatccg tttcgtggaa
    19321 gataactggg aaaacccgac gctgggtgcc tgggggctgg gctgggaagt gtggctgaac
    19381 ggcatggaag tgacgcagtt cacttacttc cagcaggttg gtggtctgga gtgtaaaccg
    19441 gttaccggcg agatcaccta cggtctggaa cgtctggcaa tgtacattca gggcgtagac
    19501 agcgtttacg acctggtctg gagcgacggc ccgctgggta aaaccaccta tggcgacgtg
    19561 ttccatcaga acgaagtgga gcagtccact tacaacttcg aatacgcgga tgtggacttc
    19621 ctgttcacct gctttgagca gtacgagaaa gaagcgcagc agctgctggc gctggaaaat
    19681 ccgctgccgc tgccagccta cgagcgtatt ctgaaagccg cccacagctt caacctgctg
    19741 gatgcgcgta aagccatctc cgtcaccgag cgtcagcgct atattctgcg cattcgcacc
    19801 ctgaccaaag cagtggcaga agcatactac gcttcccgtg aagccctcgg cttcccgatg
    19861 tgcaacaaag ataagtaaga ggcggctatg tctgagaaaa cttttctggt ggaaatcggc
    19921 actgaagagc tgccaccaaa agcactgcgc agcctggctg agtcctttgc tgcgaacttt
    19981 actgcggagc tggataacgc tggcctcgca cacggcaccg ttcaatggtt tgctgctccg
    20041 cgtcgtctgg cgcttaaagt agctaacctg gcggaagcgc aaccggatcg tgaaatcgaa
    20101 aaacgcggcc cggcgattgc ccaggcgttc gacgctgaag gcaaaccgag caaagcggca
    20161 gaaggctggg cgcgtggttg cggtattacc gttgaccagg ctgagcgtct gactaccgat
    20221 aaaggcgaat ggctgctgta tcgcgcccac gtgaagggcg aaagcactga agcactgctg
    20281 ccgaatatgg ttgcgacttc gctggcgaaa ctgccgatcc cgaaactgat gcgttggggt
    20341 gcaagcgacg tgcacttcgt gcgtccggtg cacaccgtga ccctgctgct gggcgacaaa
    20401 gttattccgg ctaccattct gggcattcag tccgatcgcg tgattcgcgg ccaccgcttt
    20461 atgggcgagc cggaattcac tatcgacaat gctgatcagt atccggaaat tctgcgtgag
    20521 cgcgggaaag tcatcgccga ttacgaagaa cgtaaggcga agattaaagc cgatgccgaa
    20581 gaagcggcgc gtaagattgg cggtaacgct gacttaagcg aaagcctgtt ggaagaagtc
    20641 gcttcgctgg tggaatggcc ggttgtgctg accgcaaaat tcgaagagaa attcctcgcg
    20701 gtgccgtctg aagcgctggt ttacaccatg aaaggtgacc agaaatactt cccggtgtat
    20761 gcgaacgacg gcaaactgct gccgaacttt atcttcgttg ccaacatcga atcgaaagat
    20821 ccgcagcaaa ttatctctgg taacgagaaa gtcgttcgtc cacgtctggc ggatgccgag
    20881 ttcttcttca acaccgaccg taaaaaacgt ctggaagata acctgccgcg cctgcaaacc
    20941 gtgctgttcc agcaacagct gggtacgctg cgtgacaaga ctgaccgcat ccaggcgctg
    21001 gctggctgga ttgctgaaca gattggcgct gacgttaacc acgctacccg tgcgggcctg
    21061 ctgtccaagt gcgacctgat gaccaacatg gtcttcgagt tcaccgacac ccagggcgtt
    21121 atggggatgc actacgcgcg tcacgatggc gaagcggaag atgttgccgt ggcgctgaac
    21181 gagcagtatc agccgcgctt tgccggtgat gacctgccgt ctaacccggt agcctgtgcg
    21241 ctggcgattg ctgacaagat ggatactctg gcgggtatct tcggtatcgg ccagcatccg
    21301 aaaggcgaca aagacccgtt tgcgttgcgt cgtgccgcac ttggcgtgct gcgtattatc
    21361 gttgagaaga acctcaacct tgatctgcaa acgctgaccg aagaagcggt gcgtctgtat
    21421 ggcgataagc tgactaatac caacgtagtt gatgatgtta tcgactttat gctcggtcgc
    21481 ttccgcgcct ggtatcagga cgaaggttac accgttgata ccatccaggc ggtactggcg
    21541 cgtcgtccga ctcgtccggc agatttcgat gcccgtatga aggcggtatc gcacttccgt
    21601 actctggatg cggcagcgtc actggcggcg gcaaacaagc gtgtatctaa cattctggcg
    21661 aagtctgacg aagtgctgag cgatcgcgtg aatgcttcta ctctgaaaga gccggaagaa
    21721 attaaactgg cgatgcaagt tgtggtgctg cgcgacaagc tggagccgta cttcgctgaa
    21781 ggtcgttacc aggacgcgct ggtcgaactg gctgaattgc gtgaaccggt tgatgccttc
    21841 ttcgataaag tgatggtcat ggttgatgac aaagaattgc gaatcaaccg tcttaccatg
    21901 ctggagaaac tgcgcgaatt attcctgcgc gttgcggata tttcgctgtt acagtaataa
    21961 cgccgttatt aaaaagcctg ccatctggca ggctttttta tcgctaaata atagagcaac
    22021 ctttaataat cttctgctga ataaagattg tctcatatat taattttatg agattttttt
    22081 aggattatat caaggagaag aaacgaactt attaagctag aatagctgtg ggtgcttgag
    22141 actgtttgtc tcaggtattc accgaaaggc agacagagaa aagccccacc tgactataaa
    22201 tcaaagtgag gctaccctat gcctgaacac cataaggtta gcctcttact cgttggaaat
    22261 caacacagga ggctgggaat gccgcagaaa tatagattgc tttctttaat agtgatttgt
    22321 ttcacgcttt tattttttac ctggatggta agagattcac tgtgtgaatt gcatattaaa
    22381 cagggaagtt atgagctggc ggcattttta gcctgcaatt tgaaagagta agagactttg
    22441 gcgggaagtt attcccgcct ttcttacggc gttgcgcatt ctcattgcac ccaaatttat
    22501 tcttcacaaa aataacacta gattctatta cgcgatcgat tatttatttc ctgaaaaaca
    22561 aataaaaaaa tccccgccaa atggcaggga tcttagattc tgtgctttta agcagagatt
    22621 acaggctggt tacgttacca gctgccgggc ctttagcgcc gctttcgatg gtgaaggaca
    22681 ctttctgacc ttcgtccaga gatttgtaac catcgttctg gatagcagag aagtgtacga
    22741 acacgtcttt agagccatcg tcaggagtga tgaagccgaa gcctttgtca gcgttgaacc
    22801 attttacgat accagtcatt ttaccggaca tagtgtatta cctttaataa ttaagtgtgc
    22861 ctttcggcga tatggcgtgc tttacagatt ttgaagcgtt aaaggaatgt gcactacgag
    22921 gggtatcaac gataactctt gaagggactt gccttactac actgctttaa tggtctgtac
    22981 gtcaaaccgt tgatgtgcat taagccacgc attggcgggt gatgcaacaa ttatttttca
    23041 tatttatgat taatcggttg gaaaacggtc ctgtcatcag gaccgtaaac agcaataaag
    23101 tggataaaag tctattccat caactgctta cttaatgccg ggttggcttg aatcaaacgc
    23161 atcaatttta gttcggcact tgaaggcttc acgcgtctgg attcccattc ctttaccatg
    23221 gcgactgata cgcccaaaac ccgggcgaaa tcatcgattt ttaaccctgt cccttttcgt
    23281 aactgctcaa tttcggtaca ggacgttgtt ctgtgcgtca gggtaatttt ctgcgtttca
    23341 tctttaaaaa caatctgttc caggctgctc aacagctcat gcattggatc tttatattcc
    23401 attgaaaact cctcaaatca cactgcggga tcgtgaattg catcgaagct cattaagaat
    23461 agtcgggaaa aacagacata gcggggtgta gtcagggcat gctctggcta tgattcgatt
    23521 cagccagtca tttagcgtga cttatagcca cctaacgatc tgataaatgc cgatatatct
    23581 cttaccactc ccggtttgta aagatttcga aaatctaacg ttctgcgaag gcgtaaatct
    23641 caggctacaa aaaaagcaac ggcactatca ttttttaaca atgaaacaac aaattgacct
    23701 tgtttcattt tctccatgat tttaccagga agattacgta gaaagggtat cctgcaagcc
    23761 tgtcctggac tatccttgtc acgtcagaca cgcgtgtgtc gttgtgcgct ttttttgggt
    23821 gaaaggagta agaaaatggc gacaggaaag tcctgctctc gctggtttgc gcctcttgcg
    23881 gcgttattaa tggtagttag cctgagtggg tgttttgata aagaaggcga tcagcgtaaa
    23941 gcgtttatcg acttcctgca gaatacagtg atgcgtagcg gtgaacgtct accaaccctg
    24001 actgccgatc agaaaaaaca gtttggtcct tttgtctctg attacgcgat tctgtatggt
    24061 tattctcagc aggtgaatca ggcgatggat tccggtctgc gcccggttgt agacagcgtt
    24121 aacgcaattc gcgtaccgca ggattacgtt acgcaaagcg gcccactgcg tgaaatgaac
    24181 ggttctctgg gcgtactggc gcaacagctg caaaatgcga agttgcaggc cgatgctgcg
    24241 cactctgcgc taaaacagag cgatgacctg aaaccggtct tcgatcaggc attcaccaaa
    24301 gtggtaacga cgccagctga tgcgttgcaa ccgttgattc cggcggcaca aactttcacg
    24361 caacaactgg tgatggttgg agactacatt gctcagcaag gtactcaggt gagctttgtc
    24421 gcaaatggca tccagttccc gacttcgcag caggcaagtg aatataacaa actgattgcg
    24481 ccattaccgg cacaacatca ggcgtttaat caggcctgga ctacagcagt tactgcaacc
    24541 caataaagag taaaagcccg agcgattatc gggctttact ccacgcagtc gcggcttagt
    24601 ccgcgacgtg cggattcaca cagttcttct caacctttcc ttgtaacgca tcaatcaagt
    24661 tatccacggc acaggcggcc atgccataac gcgtctcatg agtggcagat ccaatatgcg
    24721 gtactgcgac gacgttggcc attgagagca acggcgaatc tactgacagt ggctcttgtt
    24781 cgaagacatc cagcccggca gcgtgaattt cccctttctg caatgcagca atcagtgcat
    24841 tttcatcaac caccgggcca cgtccggcat taatgaaaat ggcggaggat ttcattttgg
    24901 cgaattgttc tgcgccaaac agatgatgcg tttcatcagt taacggcagg atcaggcaaa
    24961 cgaaatctga ctcttgtaac agtgtatcca aatcgcagta gcgggcgttg aagcgttctt
    25021 ctgcttcttt atggtggcgg cgcgcgttat agaggatggg catgttgaag ccaaagtgcg
    25081 cacgttgtgc cagcgccatg ccgatccgtc ccatcccgac aatgcccagt gttttatggt
    25141 gaacgtcagt gccgtaccag tccgggccta tgctcgcggt ccattcgcct gcttttactc
    25201 gttctgctac ctccacaacc cgacgagcgg tagacaatac cagcgccatc agcgtatcgg
    25261 cgacggtttc tgttaatacg gttggcgtgt gcatcagcag aatttttcgg gcggtaagcg
    25321 catcgacatc aaaattgtca tagcctacgg agatcgttga tgtggcacgc agtttcggca
    25381 ttttttccag caatgcggca tcaacattct cgtttgaacc cagtaaacct tcagcttcgg
    25441 caagaattgc tgcattttgt tcgatggttt gtgggctgag gtttgccacc tggtgaacgg
    25501 tgaaatgctc ttgcaggcgt tgcagtaaat catcaggtaa ggctttgtag aggataacgg
    25561 acggcttcat tcttctctcc attcactgat aaggcgatca ccttagcctg aaatatgatg
    25621 aaggaaaact gaagaatgcc aggtgatttc tgcatcacct ggcatgaaag ggattacagc
    25681 gggcttaagg taatttctac acggcggttt tgcgccttac cttctgcagt gctgttgctg
    25741 gcgattgggt tagccgggcc aaggccctga gtacggatgc ggctggcgtc cacgccctgg
    25801 gtgatcaacg cgctggcaac ggaatccgca cgctgctggg agagacgcat gttcaggtcg
    25861 tgcccacccg tgctgtcggt ataaccaatc acgttaaccg ccgttttcgg atactctttc
    25921 agtaccattg ccacgccggt cagggtgtta gcgcccgccg gtttcagggt tgcgctgctg
    25981 ctgtcgaagg tcacattgtt cggcatattg aggataatgt tatccccgct gcgggttacg
    26041 ctaacaccag tgccgcgcat tttgtcacgc agcttcgctt cctgcacatc catgtaataa
    26101 cccacgccgc cgcccagagc tgcgcctgct gctgcgccaa tgagcgcgcc tttaccgcga
    26161 tctttcttcg aagaagagag cgcaccaata cccgcgccaa cgagagagcc cagacctgcg
    26221 ccgatagcag atttacctgc ttcgcgttcg ccggtgtaag ggttagttgt acagccagat
    26281 accgccagag cgccactcac tacggcggca ataagataaa cacgtttctt cattgttaat
    26341 ccttaataac ctttttattc tttgccacgg gttctgtggc gggagattat gccgcgtgaa
    26401 catgaagatt attcctggga atactcggaa atttgtaagt aatatttaac tgctcaatac
    26461 atctaacctt tcaggagtct tcggtttggc caactcatcc tcacgatatt ccgttctgac
    26521 tgccgcccac tgggggccta tgctggttga aaccgacggc gaaaccgtgt ttagctcgcg
    26581 tggcgcgtta gccacaggaa tggaaaactc cttgcagagc gcggttcgcg accaggttca
    26641 cagcaatacg cgggtgcgat ttccaatggt gcgcaaaggc tttcttgcgt caccggaaaa
    26701 tccgcaaggc attcgtgggc aggatgaatt tgttcgcgtg agttgggatg aggcgctgga
    26761 gcttattcac catcaacata aacgcattcg tgaggcttat ggtccggcat cgatttttgc
    26821 tggttcctac ggctggcgtt caaacggcgt gctgcataag gcctcgacat tattacaacg
    26881 ctatatggcg ctggcaggcg gttatactgg gcatctgggg gattattcga ccggcgcggc
    26941 acaggcgatc atgccgtatg tcgtgggtgg cagcgaagtt tatcaacagc aaaccagttg
    27001 gccgctggtg ctggaacata gcgatgtcat ggtgctgtgg agtgctaacc cactcaatac
    27061 gctgaaaatt gcgtggaatg catccgatga gcaggggctt tcttactttt ccgcactgcg
    27121 tgacagcggg aaaaagctga tctgcattga tccaatgcga tcggaaacca tcgatttctt
    27181 tggcgataaa atggagtggg tggcaccaca catgggcacc gatgttgcgt tgatgctggg
    27241 gatcgcccat acgctggtgg aaaatggttg gcacgacgaa gcgtttctgg cgcgttgcac
    27301 cacaggttat gccgtcttcg cctcttattt gctgggcgag agtgatggta tagcgaaaaa
    27361 cgccgaatgg gcggcagaga tttgtggtgt tggcgcagcg aaaatccgcg agctggcggc
    27421 tattttccac caaaatacaa ccatgctgat ggcaggctgg ggaatgcagc gccaacagtt
    27481 tggtgagcaa aaacactgga tgctcgtcac gctggcggca atgttggggc aaatcggcac
    27541 acccggcggc ggttttggtc tttcttacca ttttgccaat ggtggtaacc ccacgcgccg
    27601 cgctgcggtg ctctcttcca tgcagggcag tttgcctggc ggcaccgatg cggtggataa
    27661 aatccctgtt gcccgcattg ttgaagcact ggaaaacccc ggtggcgcat atcaacataa
    27721 cggtatggac cgacatttcc cggatattcg ttttatctgg tgggcgggtg gtgccaactt
    27781 tactcatcat caggatacca atcgcctgat ccgtgcctgg caaaaaccgg agctggtggt
    27841 gatctctgaa tgcttctgga cggcggcggc gaaacacgcg gatatcgttc tgcctgcgac
    27901 cacctctttt gagcgtaatg atctcaccat gaccggcgat tacagtaatc agcatctggt
    27961 gccgatgaag caagtggtgc cgccacgcta tgaagcgcgt aatgattttg atgtttttgc
    28021 cgagttaagt gaacgttggg agaagggcgg ctatgcgcgg tttacggaag gaaaaagtga
    28081 gctgcaatgg ctggaaacgt tttataacgt tgcccggcaa cgcggggcaa gccagcaggt
    28141 tgaattgccg ccatttgctg agttctggca agccaaccag ttaattgaga tgccggaaaa
    28201 cccggacagc gagcggttta ttcgcttcgc cgattttcgc cgcgatccgc aggcgcatcc
    28261 attaaaaacc gccagcggca agattgaaat cttctcgcag cgtattgccg attacgctta
    28321 tccggattgc cctgggcatc caatgtggct ggagccggac gaatggcagg gcaatgccga
    28381 accggaacag ttgcaggtac tttctgctca tccggcacat cgtctgcaca gccagctgaa
    28441 ttacagttct ctgcgcgaat tgtacgcggt ggcaaaccgt gagcctgtca ccattcatcc
    28501 tgacgatgct caggcgcgcg gcatacaaga tggcgatact gttcggctgt ggaacgcacg
    28561 cgggcaaatt cttgccgggg cagtcattag cgagggaatt aaacctggcg tgatttgcat
    28621 tcacgaaggg gcatggccgg atctggattt aaccgctgac ggtatttgta aaaacggcgc
    28681 agtgaacgtg ctgaccaaag atctccccag ctcgcggctg gggaatggct gtgcgggtaa
    28741 tacggcgctg gcatggctgg aaaaatacaa cggtccggaa ctgacactta cagcgtttga
    28801 accaccggcc agctcataat ccaggtgggt agttgggttt catcctgcca tgcgcagtcg
    28861 acaatgtgaa aaccctgcgc ctggtaaaaa tctatcgccg gttgattttt ttgataaacc
    28921 tccagcatca gatggggatg gcgctgctgc acatactgca ttagcgcctt accaataccg
    28981 cgtctgacgg ccttcggtgc gacaaacatt gcagccagaa atcggccttc cataatgctg
    29041 acaaaaccga gaagcttacc gtcttcttcc cagacccagt tttgcgcgtt ggcaagatac
    29101 gcatcgcgca ccagcggaat gcagtcacgc cagtaactcg attttataaa gggatgcccc
    29161 caggttgtac tttccagcca cagttcgagg atcgcgggga gttctgaatg ttgcacttcc
    29221 cgaatcatgg tttatttccc ggatggcaac agcagccaac cacatgatca ttcaccagcc
    29281 cacatgcctg cataaaggag taacagattg tggtaccgac aaacttaaaa ccacgttttt
    29341 ttagtgcctt agatagggcg tcggaggcgg gtgtagatgt ggggatttcg ctcaacgttg
    29401 tggcttgtgt cacctgtggt tgatgattga caaacgacca gacaaagtcg gcaaacggtt
    29461 cgccgttctg ttccatttgc aggtacgccc gcgcattacc aataattgcc tgaattttcc
    29521 cgcgatggcg gataatcccg gcgtcctgta ccagtctttc gacatcctct tcctgcattg
    29581 cagccacctt caccggatcg aactgatgaa agctggcgcg atagttttcg cgttttttga
    29641 ggacggtgat ccacgataat ccagcctgct gcccttcaag gcagatcatt tcgaacagtt
    29701 ttttactgtc agtttcaggc acgccccact cattatcatg gtaggcaata taaagcgggt
    29761 cctgactcac ccagccgcaa cgttccatac tttccctcgc tatgtacccg atcgtaaaaa
    29821 tttcagccgc ttttcttgac gcatcggtga aaaagtcaat atttaataca gggctactat
    29881 gcccgatatc cttcatacaa tgacaataat attgccgaat tcggctcttc cctggagtcg
    29941 tgttaatgat cataaaaaaa agcggtggtc gctggcagct aagcctgctg gcgagtgtgg
    30001 taatcagtgc cttttttctc aacacagctt acgcctggca acaagaatat atcgttgata
    30061 cgcaacccgg acattccaca gagcgttaca cctgggatag cgatcatcaa cctgattaca
    30121 acgatatttt gtcgcaacgt attcaaagta gccaaagggc gctgggactg gaagtcaatc
    30181 tggcggaaga aactcctgtg gatgtgacca gcagtatgag tatgggctgg aattttcctt
    30241 tatatgagca ggttacaacc ggcccggtcg cggcattaca ttacgatggc acaaccacct
    30301 cgatgtataa cgagtttggc gacagtacta ccacgctggc cgatccgtta tggcatgcca
    30361 gcgtgagtac cttaggctgg cgtgttgact cccggcttgg cgatctccga ccctgggcgc
    30421 aaatcagcta taaccagcaa tttggcgaga atatctggaa ggcgcaatca ggcctgagcc
    30481 ggatgacggc gacaaaccag aacggcaact ggctggatgt caccgtaggc gctgatatgt
    30541 tgctcaatca aaatattgct gcctatgctg cgctatctca ggcagaaaat accactaaca
    30601 atagcgacta tctgtatacg atgggggtta gcgccagatt ttaacgttat agatacaatt
    30661 gaaaccaata aataacaact aatgtggcga tagtgggtga taacttacta aataataaat
    30721 ttggtgaata attgtcgagt cattcattcc tgaactaatg catttcattc cgttctgatg
    30781 gcatttcatg ccgtttttcc ccaggcataa agtgcacttc gttatggttg tcagcagaga
    30841 tttttccttt ttattactgc aggaatactg ccatgacacc ttcaaattat cagcgtaccc
    30901 gctggttgac actcatcggt actatcatta cccagtttgc ccttgggtcg gtttatacct
    30961 ggagcctgtt taacggcgcg ctttctgcca agctggatgc gccggtaagc caggtcgctt
    31021 tctctttcgg cttgttaagt ctggggctgg caatttcatc ttctgttgcg gggaaattac
    31081 aggaacgttt tggcgttaaa cgcgtcacta tggcttccgg cattttgctg ggattaggct
    31141 tcttcctgac agcgcattct gacaacctga tgatgctgtg gttaagcgcc ggtgtgctgg
    31201 tcggtctggc ggatggcgcg ggttacctgc tgacgctctc taactgcgtg aagtggttcc
    31261 cggagcgtaa aggtctgatc tccgcgttcg ctatcggttc ttatggtctg ggtagcctgg
    31321 gtttcaaatt tatcgacacg cagctgctgg aaacggtggg tctggaaaaa acctttgtga
    31381 tttggggagc gattgcgctg gtgatgattg tctttggcgc aacattaatg aaagacgcac
    31441 caaaacagga agtgaaaacc agcaatggtg tggtggagaa ggactacacc ctggcagagt
    31501 cgatgcgtaa accgcagtac tggatgttag cggttatgtt cctgactgcg tgcatgagtg
    31561 gtctgtatgt gattggtgta gcgaaagata tcgctcaaag tctggcgcat cttgatgcaa
    31621 tttccgcagc caatgctgtg acggttattt ccatcgccaa cctttctggt cgtctggtgc
    31681 tgggcattct gtctgacaaa atcgcccgta tccgtgttat caccattggt caggtgatat
    31741 cgctggtggg tatggcggcc ctgctgtttg caccattgaa tgcagtgacg ttctttgcag
    31801 cgattgcctg cgtggcattt aactttggcg gcactatcac ggtgttcccg tcactggtca
    31861 gtgagttctt cggcctcaat aacctggcga aaaactacgg tgtgatttat ctcggtttcg
    31921 gtatcggcag catttgtggg tcgattatcg cctcactgtt tggcggcttc tatgtgacct
    31981 tctacgtgat tttcgcactg ctgattctgt cactggcgct ctctacgacg attcgtcagc
    32041 cagagcagaa aatgttgcgt gaggcgcatg gctcccttta atcatctgcc gcgagctact
    32101 gggctttagc cttagtgtga atcaaacgct tcaacgcaat gtataaacaa tgacgcgtaa
    32161 aatttgggcc agttaactgg ccctttttac tgctttttgt aacgctaaag tctcttttca
    32221 aacttgcatt tttgtaaatt tgtgcttcat gcacactctt tccccacact ttttcccttt
    32281 gctgtggtct acttattcgc gcgtgtagat tttacttatc tgactacctc cgcacttttc
    32341 ccctgccggg cctgaaaagc cgctaagcag ggtgttatca cctgtttgtc cagggtttgt
    32401 ttgcatgaga tacatcaaat cgattacaca gcagaagctg agctttttgc ttgcaatcta
    32461 tattggcctt tttatgaatg gcgcggtttt ttaccgccgc ttcggcagct atgcgcacga
    32521 ttttaccgtc tggaaaggca tttctgctgt tgttgaactg gccgccaccg tactggtgac
    32581 cttcttttta ctacgtcttc tttcgctgtt tggccgccgt agctggcgta ttctggcgtc
    32641 gctggtggtg ctcttttccg caggtgccag ctattacatg accttcctta atgtggtcat
    32701 tggttatggc atcatcgctt ccgtcatgac caccgatatc gacctgtcaa aagaagttgt
    32761 tggtctgaac tttattctct ggttaatcgc cgttagcgcg ttgcctctta tccttatctg
    32821 gaataaccgc tgtcgttaca ccttgctccg acaactgcga accccagggc agcgtattcg
    32881 cagcctggcg gtcgtcgtac tggcaggtat tatggtttgg gcaccgattc gtttgctgga
    32941 tatccagcag aagaaagtgg agagggcgac aggtgttgat ttgccgagtt atggcggcgt
    33001 cgtcgcgaac tcttatctgc catcaaactg gctttcggca ttaggtcttt atgcctgggc
    33061 gcgggtcgat gaatcttccg ataacaattc tctccttaac cctgcgaaga agtttacgta
    33121 ccaggctcca caaaacgttg atgatacata tgtcgttttc attattggtg aaaccacgcg
    33181 ctgggatcat atgggcattt tcggctatga acgcaatacc acaccgaagc tggcccagga
    33241 aaaaaatctg gcggcgttcc gtggttactc ctgtgatacc gcaaccaaac tctctctgcg
    33301 ttgcatgttt gtacgtcagg ggggcgcgga agataatccg cagcgcacat taaaagaaca
    33361 gaacattttc gcggtattga agcagttagg attcagttct gatctctacg ctatgcaaag
    33421 cgaaatgtgg ttctacagca atacgatggc ggacaacatt gcttatcgtg agcagattgg
    33481 tgcggagcca cgtaaccgtg gcaaaccggt agatgatatg ttgctggtag acgaaatgca
    33541 gcaatcgctg gggcgcaacc cggatggtaa gcatctgatc attctgcata ccaaaggctc
    33601 acactttaac tacacccagc gttacccgcg cagctttgcg cagtggaagc cggaatgtat
    33661 tggtgttgat agcggctgta ccaaagcgca gatgatcaac tcatacgaca actcggtgac
    33721 ctatgtggat cactttattt ccagcgtgat tgatcaggtt cgcgataaga aagcgattgt
    33781 gttctatgca gctgaccatg gtgagtcaat taatgaacgc gagcacctgc acggcacgcc
    33841 gcgtgaactg gcaccgccgg aacagttccg cgtgccgatg atggtctgga tgtcggataa
    33901 atatctggaa aatccggtca atgcgcaggc atttgcgcag ctgaaaaaag aagctgacat
    33961 gaaagtgcca cgccgtcacg tagagctgta cgacaccatc atgggttgtc ttggctatac
    34021 ttcaccggat ggtggaatta acgaaaacaa caactggtgc cacatcccgc aggcaagaga
    34081 ggcggcggct aactaagagg cctgctgact ttctcgccga tcaaaaggca ttttgctatt
    34141 aagggattga cgagggcgta tctgcgcagt aagatgcgcc ccgcattcgg tgattggcgc
    34201 agcctggtag cgcacttcgt tcgggacgaa ggggtcggag gttcgaatcc tctatcaccg
    34261 accaaattcg aaaagcctgc tcaacgagca ggcttttttg catctgtagc tcatgaggaa
    34321 gagaaccccc gggggcgggg aggttcgact cgacgtaggt ctgataagac gcgccagcgt
    34381 cgcatcaggc aacggcaccg cacttattgt cggatgcggc gtgaacgcct tattcgatct
    34441 acacaccaca catttccctg tcaaatttgc gccccatatc tcactatcca ttaacggatt
    34501 tgtgacagaa accattgtct ttttttatat ttgcttaaat ctttttttgc gttacttatg
    34561 gctggcgctg gcattttccg ctgtgcgttt tagcgttcat ggtattaatt gctcagatat
    34621 ccaccttttc ctcagatttt tttcattaaa tgcataaaac ttaatcacca aatgttgcta
    34681 aatattaagc tgattgttct ggttgtgttg gcgtagcaca aatttctctg ctgcaaatag
    34741 ctgtttgaag tttttttaat ctttgtttgt ggattctcac cgttggtgta aatcccggtt
    34801 ggctgtattg acgttttcac attctgttga cagattgtag gtcacgaggg gcattttatg
    34861 gaggatccgc actgttacac tgatgttaat tagtacggca tccccacctc ataacgttga
    34921 cccgaccggg caaaaaacaa aaaaggtcag gcagcgacaa cccactgcaa agggttaaaa
    34981 caacaaacat cacaattgga gcagaataat gcgtatttcc ttgaaaaagt cagggatgct
    35041 gaagcttggt ctcagcctgg tggctatgac cgtcgcagca agtgttcagg ctaaaactct
    35101 ggtttattgc tcagaaggat ctccggaagg gtttaacccg cagctgttta cctccggcac
    35161 cacctatgac gcctcttccg taccgcttta taaccgtctg gttgaattta aaatcggcac
    35221 cactgaagtg atcccgggcc tcgctgaaaa gtgggaagtc agcgaagacg gtaaaaccta
    35281 taccttccat ctgcgtaaag gtgtgaagtg gcacgacaat aaagaattca aaccgacgcg
    35341 cgaactgaat gccgatgacg tagtgttctc gttcgatcgt cagaaaaacg cgcaaaaccc
    35401 gtaccataaa gtctctggcg gcagctacga atacttcgaa ggcatgggct taccagagct
    35461 gatcagcgaa gtgaaaaagg tggacgataa caccgttcag tttgtgctga ctcgcccgga
    35521 agcgccgttc ctcgctgacc tggcaatgga cttcgcctct attctgtcaa aagaatatgc
    35581 tgacgcgatg atgaaagccg gtacaccaga aaaactggac ctcaacccaa tcggtaccgg
    35641 cccgttccag ttacagcagt accagaaaga ttcccgtatt cgctataaag cgtttgatgg
    35701 ctactggggc accaaaccga agatcgatac gctggtcttc tctattactc ctgacgcttc
    35761 cgtgcgttat gcgaaattgc agaagaacga atgccaggtg atgccgtacc cgaacccggc
    35821 agatatcgcc cgcatgaagc aggataaatc catcaacctg atggaaatgc cggggctgaa
    35881 cgtcggttat ctctcgtata acgtgcagaa aaaaccactc gatgacgtga aagttcgcca
    35941 ggctctgacc tacgcggtga acaaagacgc gatcatcaaa gcggtttatc agggcgcggg
    36001 cgtatcagcg aaaaacctga tcccgccaac catgtggggc tataacgacg acgttcagga
    36061 ctacacctac gatcctgaaa aagcgaaagc cttgctgaaa gaagcgggtc tggaaaaagg
    36121 tttctccatc gacctgtggg cgatgccggt acaacgtccg tataacccga acgctcgccg
    36181 catggcggag atgattcagg cagactgggc gaaagtcggc gtgcaggcca aaattgtcac
    36241 ctacgaatgg ggtgagtacc tcaagcgtgc gaaagatggc gagcaccaga cggtaatgat
    36301 gggctggact ggcgataatg gggatccgga taacttcttc gccaccctgt tcagctgcgc
    36361 cgcctctgaa caaggctcca actactcaaa atggtgctac aaaccgtttg aagatctgat
    36421 tcaaccggcg cgtgctaccg acgaccataa caaacgcgtt gaactgtaca agcaagcgca
    36481 ggtggtgatg cacgatcagg ctccggcact gatcatcgct cactccaccg tgtttgaacc
    36541 ggtacgcaaa gaagttaaag gctatgtggt tgatccatta ggcaaacatc acttcgaaaa
    36601 cgtctctatc gaataattaa aagccataca agactgatgg caaaggcaaa actgcctgat
    36661 gcgctccgct tatcaggcct acgaaaactc tgcaatgtat tgagtttgca cgattttgta
    36721 ggtcggataa ggcgtttgcg ccgcatccgg cataaacaaa gcgctctttg tcaacaatct
    36781 gcatacccgg tggcgctgtg cctctgcgtg cagcgccatc cggcagcaat acttcattcc
    36841 ctgttccaca agggcgggaa ttgatttgtg agcaatacag acacgcagtt ccaggctgcg
    36901 ggtcattata gagaatccgg gttatgttgc agtttattct ccgacgtttg ggactcgtca
    36961 tccccacgtt tatcggtatt acccttctca catttgcctt tgtccacatg atcccgggcg
    37021 atccggtgat gatcatggcg ggcgagcgtg ggatctcccc agagcgtcac gcgcagctgc
    37081 tggctgaact cggcttagat aaaccgatgt ggcagcagta tctccattac atttggggcg
    37141 taatgcacgg cgatttaggc atttcgatga aaagccgcat cccggtatgg gaagagttcg
    37201 tgccgcgctt ccaggccacg ctggaacttg gcgtctgcgc gatgattttt gctacggcag
    37261 tcggtattcc ggttggtgtg ctggctgcgg ttaaacgcgg ttccattttc gatcacacgg
    37321 cggttggcct ggcgctgaca ggttattcaa tgcctatctt ctggtggggc atgatgctga
    37381 tcatgctggt ttcggtgcac tggaacctga cgcccgtctc cggtcgcgtg agcgacatgg
    37441 tgttcctcga tgattccaat ccgttaaccg gttttatgct gatcgacacc gccatctggg
    37501 gtgaagacgg taactttatc gatgccgtcg cccatatgat cttgcccgcc attgtgctgg
    37561 gtactatccc gctggcggtc attgtgcgta tgacacgctc ctcgatgctg gaagtgctgg
    37621 gtgaagatta catccgcacc gcgcgcgcca aagggttgac ccgccttagg gtgattatcg
    37681 tccatgcgct gcgtaacgcg atgctgccgg tggtgaccgt tatcggcctg caggtgggaa
    37741 cgttgctggc gggggcgatt ctgaccgaaa ccatcttctc gtggcccggt ctggggcgct
    37801 ggttgattga cgcactgcaa cgccgcgact atccggtagt gcagggcggc gtattgctgg
    37861 tggcgacgat gattatcctc gtcaacttgc tggtcgatct gctgtacggc gtggtgaacc
    37921 cgcgtattcg tcataagaag taaggggaca tcatgtcaca ggttactgaa aataaagtga
    37981 ttagcgcacc ggtgccgatg accccgttac aggagttctg gcactatttt aaacgcaaca
    38041 aaggcgcggt cgtcgggctg gtttacgtcg tcatcgtgct gtttatcgcg atctttgcca
    38101 actggattgc accctataac ccggcggaac agttccgcga tgcactgctc gccccgccag
    38161 cctggcagga aggcggcagc atggcgcact tgctgggcac cgatgacgta ggccgtgatg
    38221 tgctgtcgcg tctgatgtac ggtgcgcgat tgtcactgct ggttggctgt ctggtagttg
    38281 tgttatcgct gattatgggc gttattctcg gcctgatcgc cggttacttc ggcggcctgg
    38341 tcgataacat catcatgcgc gtggtcgata tcatgctggc gctgccaagt ctgctgttgg
    38401 cactggtgct ggtggcgatt ttcggcccgt cgattggtaa cgccgcgctg gcgctgacct
    38461 ttgttgccct gccgcactat gtgcgcttaa cccgcgccgc cgtgctggtg gaagttaacc
    38521 gcgattacgt taccgcgtct cgcgtggcag gtgccggggc gatgcgtcag atgtttatta
    38581 acatcttccc gaactgcctt gcgcctctga ttgttcaggc gtcgctcggt ttctctaacg
    38641 ccattctcga tatggctgct cttggtttcc tcggcatggg ggcgcagccg ccaacgccgg
    38701 agtggggcac catgctctcc gacgtgttgc agttcgcgca aagcgcctgg tgggtcgtga
    38761 ccttcccggg tctggcgatc ctgctgacgg tgctggcatt taacctgatg ggtgacggtc
    38821 tgcgtgacgc gctcgatccc aaactgaagc agtaagaggt tcgagatggc gttattaaat
    38881 gtagataaat tatcggtgca tttcggcgac gaaagcgcac cgttccgcgc cgtagaccgc
    38941 atcagctaca gcgtaaaaca gggcgaagtg gtcgggattg tgggtgagtc cggctctggt
    39001 aaatcggtca gttcgctggc gattatgggg ctgattgatt atccgggccg cgtaatggcg
    39061 gaaaagctgg agtttaacgg tcaggatttg cagcgtatct cggaaaaaga gcgccgcaac
    39121 cttgtgggtg ccgaagtggc gatgatcttc caggacccga tgaccagcct taacccgtgc
    39181 tacaccgtgg gtttccagat tatggaagcg attaaggtgc atcagggcgg taataagagc
    39241 actcgtcgtc agcgggcgat tgacttgttg aatcaggtcg gtattcccga tccggcttcg
    39301 cgtctggatg tttacccgca tcagctttcc ggcggcatga gccagcgcgt gatgattgcc
    39361 atggcgattg cctgtcggcc aaaactgctg attgccgatg aaccaaccac tgcgctggac
    39421 gtgaccattc aggcgcaaat cattgaacta ctgctggagc tacagcagaa agagaacatg
    39481 gcgctggtgt taattaccca tgacctggcg ctggtggcgg aagcggcaca taaaatcatc
    39541 gtgatgtatg ccggtcaggt ggtggaaacg ggcgatgcgc acgccatctt ccatgcgccg
    39601 cgtcacccgt atactcaggc attgctgcgt gcgctgccgg aatttgctca ggataaagaa
    39661 cgtctggcgt cgttgccagg tgttgttccc ggcaagtatg accgcccgaa cggctgcctg
    39721 cttaacccgc gctgccccta tgccactgac agatgtcgcg ctgaagaacc ggcgctgaat
    39781 atgcttgctg acgggcgtca gtccaaatgc cattacccac ttgatgatgc cgggaggccg
    39841 acactatgag tacgcaagag gccacctcgc aacaaccgct gttgcaggct atcgacctga
    39901 aaaaacatta tccggtgaag aaaggtatgt tcgcgccgga acgtttggtt aaagcgctgg
    39961 acggcgtttc gtttaacctt gaacgcggca aaacgctggc ggtagtaggg gaatctggct
    40021 gcggtaaatc gaccctcggt cggttgctga cgatgatcga aacgcccacc ggtggtgaac
    40081 tgtattacca agggcaggat ctgctcaagc acgatccgca ggcgcagaag ctgcgtcggc
    40141 agaaaatcca gattgtcttc cagaaccctt acggttcgct gaatccacgt aaaaaagtcg
    40201 ggcaaattct tgaggagccg ctgctgatta ataccagctt aagcaaagaa cagcgtcggg
    40261 aaaaagccct gtcgatgatg gcgaaagtcg gcctgaaaac cgaacattat gaccgctatc
    40321 cgcatatgtt ctccggcggt cagcgtcagc gtatcgccat tgcccgaggt ctgatgctcg
    40381 acccggatgt ggtgattgcc gatgagccgg tttccgcgct ggacgtctca gtgcgcgcgc
    40441 aggtgctgaa tctgatgatg gatttgcagc aggagttggg gctgtcttat gtctttatct
    40501 cgcacgacct gtcggtggta gagcacattg ctgatgaagt gatggtgatg tacctgggcc
    40561 gctgcgtgga gaagggaacg aaggaccaaa tcttcaataa cccgcgtcat ccgtacactc
    40621 aggcgctact ctccgcgacg ccgcgcctga acccggacga tcgccgcgag cgcatcaagc
    40681 tcaccggtga actgccaagc ccactgaatc caccgccggg ctgcgcgttt aacgcccgct
    40741 gtcgtcggcg cttcggcccc tgcacccagt tgcagccgca gctaaaagac tacggcggtc
    40801 aactggtagc ttgttttgct gttgatcagg atgaaaatcc gcagcgttaa ctcacaaaac
    40861 cggggccgtg cgctccggtt tactcaatca gcttcaacag tggcgacaag catagcaaaa
    40921 tgccgtaaag cagaatcagc caggctttaa acccgcgaag tttttccagt tgtgcgactt
    40981 tatagatcag gaaaaatgga atgaggcacg acacaatgcc atataacggg cttcccaact
    41041 gaaagaacac cagcaccgat acacgaaacg agacccaaat cgtcaacgta atgacgataa
    41101 aagcgcagat cgccagagtc agcatgcgtg agttaatttt cttggtatca ataattcggc
    41161 ttaacagatt gagaataatg cctttaatgg cctcgtggaa accgagataa atgccaaaga
    41221 atgcggtcag tacggcaaag atattcagca ccgtcgaggt gatatgaatg atatgcccag
    41281 ggatcacctg cgcggccagt gccagtgccg agatattttg ttcaaaggca gaaacggctt
    41341 cttcgtggct aattgagaag gtaaacgaaa aggcaaaaaa taggatcacc gcgatgagcg
    41401 tgacataact aattcggtgg gtacgcagcg cgagccgggt tgccagtact ttatccgctt
    41461 cccgtttgcg gtaggcaata ttcatcgggt ttagcacctg aataaatacc gcagaaaaga
    41521 agcaaaaagg aatagtaagc aggacgtcgc ggaagaagac ggaggcttgc gggaaggcgg
    41581 ttatattggc gaaattccag tgcgggatca tcgcaaaacc gaagacgaca ataatcccta
    41641 ctttgaccac caccattggc ccggaaatct taaacagtaa tcgttcacca ccagacgcaa
    41701 ttgccaccag tacggcgaaa atggcgactt tataaagtag agattgtgaa agatcggcat
    41761 cggttaaacc gaaggttttc aggtacgagg cgctgtcgaa aaccacggag agagagtaga
    41821 taaaaatccc gtggataatc atcaaaaagt agataacccc gaggaaaatt ccccagttct
    41881 tccccaggta atgactgata atatcggtgt agtcattaca ggaatcgctt tcagaaaggg
    41941 tttttaaata aatgtcctgc actacccagg tggcaggata agcaatgatc gctgcggtaa
    42001 taaataccca aattcccttc aagccaattt gtactggcat cagcacggtt ccggcaccaa
    42061 tcgccatgcc tatgcataat aaaacccagc cgaagtcgta gcgtgtaaac ggcattttct
    42121 gatcgtgttt cggtagtgtg ttgtgctgca taaagtcctg tccagaaaga agtcggcctc
    42181 gcaaaaatga ataaacccag tttctcttgc gcaacaggtg gagaagtcga atacgcagta
    42241 tgtaaaaaat gaggcaacag attcactggg gaggtcgcga ggaggcgcaa ttctacaaaa
    42301 ttgcgcattt cactaccagt ctggagtcag cccggatatg aactgcaact aatgcaaata
    42361 aagaaattaa gctggaaggc atcacgcaat gaggaagatt gccgggtgct gacggtaaaa
    42421 agcaatcgta aaatcagcgg caagccggaa aggttccggt gaggcgcaat gttgcggggg
    42481 cttaatccct ggtggcattg gttgctggag agagaaaacc cccgcacgtt gcaggtatgc
    42541 acctgacaac atcacggggg ctaatcttga acctagacca ctcaagaata gccgcgaaac
    42601 gttgtcatta caacacaggc ggctatatga cgctcgcaga actgggcatg gccttctggc
    42661 atgatttagc ggctccggtc atagctggca ttcttgccag tctgatcgtg aactggctga
    42721 acaagcggaa gtaacgtgtc agacgggcgt caggctgccg taatggcaat ttgcgcccgg
    42781 atcaggccgc agggataaaa ctctgcggcc tttttcgtaa gaaaaatgca acggttgcaa
    42841 gcggataata ttctgcggaa tgcacagcac agagtgaaga agtgacggat ttcggagctg
    42901 aaaaacagtc gtagaatcag cggcaagccg gaaaggttcc ggtgaggcgc aatgttgcgg
    42961 gggcttaatc cctggtggca ttggttgctg gagagagaaa acccccgcat attgcaggta
    43021 tgcacctgac aacatcacgg gggccattct tgaacctaca caa
//
LOCUS       DAZGQL010000032        42591 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.032, whole genome shotgun sequence.
ACCESSION   DAZGQL010000032 DAZGQL010000000
VERSION     DAZGQL010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42591
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.032"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..1116
                     /locus_tag="ACHKIR_002016"
     CDS             <1..1116
                     /locus_tag="ACHKIR_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HFO9989760.1"
                     /translation="RYAAIFLALPLLCTTAQANSFLHYAGEFNFRTGEKQNNVTIAGL
                     SAITFDAKKNIFYAINDSRNNNKEGDASLYTLKIQVSSRGIEQVNFLNQRPLLDAKQQ
                     PFVTNTVDAEGLALTHNGKSLLWSSELGAPLRLSTLDGVMEKDFTSLFPARFNISSDK
                     ESSNGIRSGNAWEGLTVTPDGKSLFIAVESSLKQDGPIASPINSGTSRLLQFSIDADG
                     RPSKQLHEYLYITDPVPQVSKFGINDNGVSEVMALNDHQLLVIERSGRNVSAGFNDWD
                     YSVRVYMVDLTAASDIKNIDSLQDWSNKSTLQPVSKKLLIDFADYTSSADCIEGVTFG
                     PLIDGHTSLIFVSDNNFQPHQQTKFYLFIDKENKLKI"
     gene            1212..1946
                     /locus_tag="ACHKIR_002017"
     CDS             1212..1946
                     /locus_tag="ACHKIR_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001317336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligogalacturonate-specific porin KdgM family
                     protein"
                     /protein_id="HFO9989761.1"
                     /translation="MNSYLKISLAGITMLSCLSPTLVCAEQPHGFIDYRHEYLDDTRT
                     HADRVEFGTFFSNGIGLMGELRYNTDEGDKDKWDPSQFGNNGTGLSVVYRFKPLDDKK
                     FWLEPMFWLDTTQYWSTYEYGLSAGYDFSKEWKVSGRFRYDMDKATDKSKGYGNDDRN
                     NRRYDVWIDYRPQKTNFQYQLNLVYYNNGYLTWNDGHKNYTASFKVGYKMGSWIPYMS
                     IADYKGVDKTSSNRQIRWRWGLTYTF"
     gene            1960..2829
                     /locus_tag="ACHKIR_002018"
     CDS             1960..2829
                     /locus_tag="ACHKIR_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001060172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2608 domain-containing protein"
                     /protein_id="HFO9989762.1"
                     /translation="MNNKFKIILWMMLSALPALSYAQVKNIVTGEYSAVSKIITEKET
                     LYPSDKILLVFDIDNTLLTSGTRIGGDIWYQWQTDKLPLKPDDSQKVPCLYENTISML
                     YELSPMQLTEPQLPTMLKQWQQKHTAFALTSRAPDTLFPTLRELKRNGIDFSSSALRK
                     KEDTLPPFEKGKLKRSWLYSQGVFFSSGQDKGVILDYMLDKMGNKYDAIVFIDDGQAN
                     IHAMTKMFSKDKWSSTDFTIIHYTRVEDELIKQQGAVITTAQAEKMATDWKTLAGSLA
                     TLFPDRNKLCPIK"
     gene            complement(2874..4766)
                     /gene="parE"
                     /locus_tag="ACHKIR_002019"
     CDS             complement(2874..4766)
                     /gene="parE"
                     /locus_tag="ACHKIR_002019"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFO9989763.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFTLWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(4795..5376)
                     /gene="yqiA"
                     /locus_tag="ACHKIR_002020"
     CDS             complement(4795..5376)
                     /gene="yqiA"
                     /locus_tag="ACHKIR_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HFO9989764.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(5376..6203)
                     /gene="cpdA"
                     /locus_tag="ACHKIR_002021"
     CDS             complement(5376..6203)
                     /gene="cpdA"
                     /locus_tag="ACHKIR_002021"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004115 - 3',5'-cyclic-AMP
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HFO9989765.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(6228..6650)
                     /gene="yqiB"
                     /locus_tag="ACHKIR_002022"
     CDS             complement(6228..6650)
                     /gene="yqiB"
                     /locus_tag="ACHKIR_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HFO9989766.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(6651..7280)
                     /gene="nudF"
                     /locus_tag="ACHKIR_002023"
     CDS             complement(6651..7280)
                     /gene="nudF"
                     /locus_tag="ACHKIR_002023"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HFO9989767.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            7485..8966
                     /gene="tolC"
                     /locus_tag="ACHKIR_002024"
     CDS             7485..8966
                     /gene="tolC"
                     /locus_tag="ACHKIR_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HFO9989768.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNTLLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            9114..9785
                     /gene="ygiB"
                     /locus_tag="ACHKIR_002025"
     CDS             9114..9785
                     /gene="ygiB"
                     /locus_tag="ACHKIR_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HFO9989769.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCIAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            9791..10951
                     /gene="ygiC"
                     /locus_tag="ACHKIR_002026"
     CDS             9791..10951
                     /gene="ygiC"
                     /locus_tag="ACHKIR_002026"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFO9989770.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            11436..13232
                     /locus_tag="ACHKIR_002027"
     CDS             11436..13232
                     /locus_tag="ACHKIR_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aryl-sulfate sulfotransferase"
                     /protein_id="HFO9989771.1"
                     /translation="MFDKYRKTLVAGTVAITLGLSASGVMAAGFKPAPPAGQLGAVIV
                     DPYGNAPLTALVDLDSHVISDVKVTVHGKGEKGVEISYPVGQESLKTYDGVPIFGLYQ
                     KFANKVTVEWKENGKVMKDDYVVHTSAIVNNYMDNRSISDLQQTKVIKVAPGFEDRLY
                     LVNTHTFTAQGSDLHWHGEKDKNAGILDAGPATGALPFDIAPFTFIVDTEGEYRWWLD
                     QDTFYDGRDRDINKRGYLMGIRETPRGTFTAVQGQHWYEFDMMGQVLEDHKLPRGFAD
                     ATHESIETPNGTVLLRVGKSNYRRDDGVHVTTIRDHILEVDKSGRVVDVWDLTKILDP
                     KRDALLGALDAGAVCVNVDLAHAGQQAKLEPDTPFGDALGVGPGRNWAHVNSIAYDAK
                     DDSIILSSRHQGVVKIGRDKQVKWILAPSKGWEKPLASKLLKPVDANGKPITCNENGL
                     CENSDFDFTYTQHTAWISSKGTLTIFDNGDGRHLEQPALPTMKYSRFVEYKIDEKKGT
                     VQQVWEYGKERGYDFYSPITSIIEYQADRNTMFGFGGSIHLFDVGQPTVGKLNEIDYK
                     TKEVKVEIDVLSDKPNQTHYRALLVRPQQMFK"
     gene            13250..13918
                     /locus_tag="ACHKIR_002028"
     CDS             13250..13918
                     /locus_tag="ACHKIR_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HFO9989772.1"
                     /translation="MSKLGISSLFKTILLTAALAVSFTASAFTEGTDYMVLEKPIPNA
                     DKTLIKVFSYACPFCYKYDKAVTGPVSEKVKDIVAFTPFHLETKGEYGKQASEVFAVL
                     INKDKAAGISLFDANSQFKKAKFAYYAAYHDKKERWSDGKDPAAFIKTGLDAAGMSLA
                     DFEAALKEPAVQETLEKWKASYDVAKIQGVPAYVVNGKYLIYTKSIKSIDAMADLIRE
                     LASK"
     gene            13933..14604
                     /gene="dsbI"
                     /locus_tag="ACHKIR_002029"
     CDS             13933..14604
                     /gene="dsbI"
                     /locus_tag="ACHKIR_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000511507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide oxidoreductase DsbI"
                     /protein_id="HFO9989773.1"
                     /translation="MGIKGMWKDLRTSPVDTLVRWQEQRLLWLLMAVAMGALIILAHS
                     FFQIYLYMAPCEQCVYIRYAMFVMVIGGLVAAINPKNIILKLIGCVMAFYGSILGLKF
                     SLKLNDIHHAVHNPDPDSLFGVQGCSTDPTFPFNLPLAQWAPNWFKPTGDCGYDAPIV
                     PDGVTLSSTQQWFVEMYQQSEGWYLLPPWHFMNMAQACMLAFGMCLVLLVIMSGAWAL
                     KIVRG"
     gene            complement(14650..15438)
                     /gene="ygiD"
                     /locus_tag="ACHKIR_002030"
     CDS             complement(14650..15438)
                     /gene="ygiD"
                     /locus_tag="ACHKIR_002030"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HFO9989774.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQTLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQRLVELLAPIPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            15581..16354
                     /gene="zupT"
                     /locus_tag="ACHKIR_002031"
     CDS             15581..16354
                     /gene="zupT"
                     /locus_tag="ACHKIR_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HFO9989775.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(16412..16582)
                     /gene="yqiD"
                     /locus_tag="ACHKIR_002032"
     CDS             complement(16412..16582)
                     /gene="yqiD"
                     /locus_tag="ACHKIR_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiD"
                     /protein_id="HFO9989776.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(16844..17497)
                     /gene="ribB"
                     /locus_tag="ACHKIR_002033"
     CDS             complement(16844..17497)
                     /gene="ribB"
                     /locus_tag="ACHKIR_002033"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HFO9989777.1"
                     /translation="MNQTLLSSFGTPFERVENALVALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(17601..17749)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            17871..18170
                     /gene="ubiK"
                     /locus_tag="ACHKIR_002034"
     CDS             17871..18170
                     /gene="ubiK"
                     /locus_tag="ACHKIR_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311957.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HFO9989778.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRISELEARNNSVADLQSPPAIPPIDKAE"
     gene            complement(18205..18405)
                     /gene="glgS"
                     /locus_tag="ACHKIR_002035"
     CDS             complement(18205..18405)
                     /gene="glgS"
                     /locus_tag="ACHKIR_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HFO9989779.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQTRELELEH"
     gene            18674..19303
                     /gene="yqiJ"
                     /locus_tag="ACHKIR_002036"
     CDS             18674..19303
                     /gene="yqiJ"
                     /locus_tag="ACHKIR_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HFO9989780.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEIFALICGHMLSGALDAHLD
                     HYDSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHTCLMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLLEPEEGKIFTKGDKVLIICRLSATRYLAENNPWPQIL"
     gene            19330..20991
                     /gene="yqiK"
                     /locus_tag="ACHKIR_002037"
     CDS             19330..20991
                     /gene="yqiK"
                     /locus_tag="ACHKIR_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFO9989781.1"
                     /translation="MDDIVNSVPSWMFTAIIAVCILFIIGIIFARLYRRASAEQAFVR
                     TGLGGQKVVMSGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFF
                     VRVKPSVEGIATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENF
                     VQGVQNTVAEDLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRR
                     ERNEVEQDVEVAVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNAKIAAFEA
                     ERRREAEQTRILAERQIQETEIDREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKS
                     IAIAAKSEQQSQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETK
                     AVELTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLK
                     FKLALLQALPAVIEKSVEPMKSIDGIKIIQVDGLNRGGATGDANTGNVGGGNLAEQAL
                     SAALSYRTQAPLIDSLLNEIGVSGGSLAALTSSLSSTTPVEEKAE"
     gene            21231..21290
                     /locus_tag="ACHKIR_002038"
     CDS             21231..21290
                     /locus_tag="ACHKIR_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO9989782.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            complement(21410..22843)
                     /gene="hldE"
                     /locus_tag="ACHKIR_002039"
     CDS             complement(21410..22843)
                     /gene="hldE"
                     /locus_tag="ACHKIR_002039"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HFO9989783.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNQ
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(22891..25731)
                     /gene="glnE"
                     /locus_tag="ACHKIR_002040"
     CDS             complement(22891..25731)
                     /gene="glnE"
                     /locus_tag="ACHKIR_002040"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HFO9989784.1"
                     /translation="MKPLSSPLQQYWQTVVERLPEPLAEESLSAQAKSVLTFSDFLQD
                     SIIAHPEWLTELESQPPQADEWQHYAAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSHLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSDGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPFDELNRARLAWAMDFADWPQLTGVLTAHMANVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLMLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERELVRASWQKWLVEE"
     gene            complement(25754..27055)
                     /gene="ygiF"
                     /locus_tag="ACHKIR_002041"
     CDS             complement(25754..27055)
                     /gene="ygiF"
                     /locus_tag="ACHKIR_002041"
                     /EC_number="3.6.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HFO9989785.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRGHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEV
                     WPNGELPTDLASRVQPLFSTDFYREKWLVEVDGSQIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAIGLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQSKM
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLTRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            27297..27917
                     /gene="ygiM"
                     /locus_tag="ACHKIR_002042"
     CDS             27297..27917
                     /gene="ygiM"
                     /locus_tag="ACHKIR_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HFO9989786.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            27981..29219
                     /gene="cca"
                     /locus_tag="ACHKIR_002043"
     CDS             27981..29219
                     /gene="cca"
                     /locus_tag="ACHKIR_002043"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HFO9989787.1"
                     /translation="MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLSFRIADETLALM
                     REMTHAGELEHLTPERVWKETENALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVAGWKEQRCPKPE"
     gene            complement(29407..30228)
                     /gene="bacA"
                     /locus_tag="ACHKIR_002044"
     CDS             complement(29407..30228)
                     /gene="bacA"
                     /locus_tag="ACHKIR_002044"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFO9989788.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(30319..30687)
                     /gene="folB"
                     /locus_tag="ACHKIR_002045"
     CDS             complement(30319..30687)
                     /gene="folB"
                     /locus_tag="ACHKIR_002045"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HFO9989789.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            30792..31409
                     /gene="plsY"
                     /locus_tag="ACHKIR_002046"
     CDS             30792..31409
                     /gene="plsY"
                     /locus_tag="ACHKIR_002046"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFO9989790.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(31422..32354)
                     /gene="ttdR"
                     /locus_tag="ACHKIR_002047"
                     /gene_synonym="dan"
     CDS             complement(31422..32354)
                     /gene="ttdR"
                     /locus_tag="ACHKIR_002047"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate utilization transcriptional activator
                     TtdR"
                     /protein_id="HFO9989791.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDAIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVRVLPEYAQSANIWAVYR
                     EPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQVM"
     gene            32561..33472
                     /gene="ttdA"
                     /locus_tag="ACHKIR_002048"
     CDS             32561..33472
                     /gene="ttdA"
                     /locus_tag="ACHKIR_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008730 - L(+)-tartrate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HFO9989792.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIVPDGDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            33469..34074
                     /gene="ttdB"
                     /locus_tag="ACHKIR_002049"
     CDS             33469..34074
                     /gene="ttdB"
                     /locus_tag="ACHKIR_002049"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HFO9989793.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            34123..35586
                     /gene="ttdT"
                     /locus_tag="ACHKIR_002050"
     CDS             34123..35586
                     /gene="ttdT"
                     /locus_tag="ACHKIR_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HFO9989794.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALIPVPAGLESHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTIVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTVDYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(35629..36642)
                     /gene="tsaD"
                     /locus_tag="ACHKIR_002051"
     CDS             complement(35629..36642)
                     /gene="tsaD"
                     /locus_tag="ACHKIR_002051"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFO9989795.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            36880..37095
                     /gene="rpsU"
                     /locus_tag="ACHKIR_002052"
     CDS             36880..37095
                     /gene="rpsU"
                     /locus_tag="ACHKIR_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFO9989796.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            37206..38951
                     /gene="dnaG"
                     /locus_tag="ACHKIR_002053"
     CDS             37206..38951
                     /gene="dnaG"
                     /locus_tag="ACHKIR_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFO9989797.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKQAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDNQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            39146..40987
                     /gene="rpoD"
                     /locus_tag="ACHKIR_002054"
     CDS             39146..40987
                     /gene="rpoD"
                     /locus_tag="ACHKIR_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFO9989798.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
     gene            41200..42015
                     /locus_tag="ACHKIR_002055"
     CDS             41200..42015
                     /locus_tag="ACHKIR_002055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9989799.1"
                     /translation="MFFLCVGKNKGWRKVQKASDYGVIILTEEQFINFIDTGEIPLEQ
                     DCVVENDISKSLEKDVNTYKSMIDEFSLSIRTVREFRRSNALIAVFVDGYAAGWRFAV
                     KECYKDSLDIKLTPVKTEFFNYEIWTQGDSFQFNRGDVFYSDRLAYDSYCDFLKLDDA
                     VVLQVKYECFSGYDSVSTFDGVMDGEFSFSRMEPRPVHKLPVLIETQLYDPGKIIVDV
                     FVPDAAKKQLKLKDTLELKQDEFVCLLQSGCCWVKRREAGDKKKPEFINLFKK"
     gene            complement(42130..42270)
                     /locus_tag="ACHKIR_002056"
     CDS             complement(42130..42270)
                     /locus_tag="ACHKIR_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO9989800.1"
                     /translation="MPQKTIIVSILCITILMIVWMIHTSPCEFRLNIMWSQFAAFLQC
                     KQ"
     gene            complement(42381..>42591)
                     /locus_tag="ACHKIR_002057"
     CDS             complement(42381..>42591)
                     /locus_tag="ACHKIR_002057"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9989801.1"
                     /translation="TKTKKSGKPELARPGGEWQQKSLLHTFAQLLRKRMAKGKEKQPR
                     HPLHVTDVKGKLRAGAFRLTAGRIT"
BASE COUNT        10798 a        11088 c        10446 g        10259 t
ORIGIN      
        1 agatatgccg ctatttttct ggcattaccg ctgttatgca caacagcgca ggcaaacagt
       61 tttcttcatt atgctgggga gtttaacttc cggacgggag aaaagcagaa caatgtcaca
      121 atcgcggggc tatcagccat cacatttgat gcaaaaaaaa atattttcta tgctattaac
      181 gatagcagaa acaataataa agaaggagat gccagtcttt atacactaaa aatacaggta
      241 tcatccaggg gcattgagca agtgaatttt ctcaaccagc gtccactgct tgatgcaaaa
      301 cagcaacctt tcgtgaccaa tacggtggat gccgaaggtc tggccctgac acacaatggt
      361 aaatcattac tctggagttc ggagttaggt gctccactgc gtctgagcac tctcgatggc
      421 gtaatggaga aagattttac gtctttattt cctgctcgct ttaatattag ttctgacaaa
      481 gagagcagca atggtattcg cagcggcaac gcctgggagg gcctgacagt tacaccagat
      541 ggaaaatcac tttttatcgc cgttgaaagc agccttaaac aggatgggcc tattgcaagt
      601 ccaataaatt caggcacatc acgcttactg caattttcta tagatgcaga cggtcgacct
      661 tcaaagcaat tgcatgaata tctctatatt actgatcctg ttccgcaagt gagtaagttc
      721 ggtattaatg ataatggcgt atccgaggtc atggcgctga atgatcacca attgttggtc
      781 atcgaacgtt ccggcagaaa cgtcagtgct gggttcaatg actgggatta ctctgtcaga
      841 gtttatatgg tcgatttaac tgctgccagc gatatcaaga atattgacag tctgcaagat
      901 tggtccaata aatccacgtt acaacccgtc agtaaaaaat tattaattga ttttgctgat
      961 tacacttcct ctgcggattg tattgaaggg gtaaccttcg gtcctctgat tgatgggcat
     1021 acatcgttaa tatttgtatc tgataataat ttccagcccc atcaacagac gaaattttat
     1081 ctattcattg ataaggaaaa caaacttaaa atttaaagtc aaactataga tacaaaaaca
     1141 ttctaataca tggaatcatc agatggtgta ttcgatcacc aaaaatatat tttattctga
     1201 aagggaatat tatgaattct tatcttaaaa tatcacttgc aggaataaca atgttatcct
     1261 gcctgtcacc tacactagtc tgtgcagaac agccgcatgg atttattgat tatcgccatg
     1321 agtatcttga tgatactcgc acccacgcgg accgcgttga atttggtaca ttttttagta
     1381 atggtatcgg tttgatggga gaattacgct ataacaccga tgaaggtgat aaagataagt
     1441 gggacccctc acaatttggc aataacggaa caggattatc tgttgtttac cgctttaagc
     1501 cgctggatga caaaaaattc tggttagaac ccatgttctg gcttgatacc acgcaatatt
     1561 ggtcaactta cgaatatggt ctttctgcgg gttatgactt cagcaaagaa tggaaagttt
     1621 caggtcggtt tcgttatgac atggataaag ccaccgataa atccaaaggt tatggcaacg
     1681 atgatcgtaa taaccgccgt tacgatgtgt ggatcgacta tcgaccacaa aaaaccaact
     1741 tccaatatca gttaaacctg gtctattaca ataatggtta tttaacctgg aatgatggcc
     1801 ataaaaacta taccgcatca tttaaagtag ggtacaaaat gggatcgtgg ataccctata
     1861 tgagtattgc ggattataaa ggtgtggata aaacatcaag taatcgccaa atccgctggc
     1921 gctggggtct gacttatact ttctaatctg gaataaaaaa tgaataataa atttaaaatc
     1981 attctctgga tgatgctctc tgctctgcct gctttatctt acgcacaggt aaaaaatata
     2041 gttactggcg aatatagtgc ggtcagtaaa attatcacag aaaaggaaac tctctatcca
     2101 tccgataaga tattattggt ttttgacatt gataacacgt tattaacgag tggcactcgc
     2161 attggcggtg atatctggta tcaatggcaa accgataaat tgcctttaaa gccggatgac
     2221 tcgcaaaaag ttccttgcct gtatgaaaat acaatcagta tgctttacga acttagccct
     2281 atgcagctta ccgagccaca gttaccgaca atgttaaaac agtggcaaca aaagcatacc
     2341 gcttttgctc tgacatcccg tgcaccggat actctctttc cgacattgcg ggaattaaag
     2401 cgaaacggca ttgatttttc gtcttccgca ctcaggaaga aagaggatac tttgccaccc
     2461 tttgaaaaag ggaaactcaa acggtcatgg ctgtacagcc agggggtatt tttttcttcc
     2521 gggcaagata aaggcgtaat tctcgattat atgctggata aaatggggaa caaatatgac
     2581 gcgattgttt ttatcgatga cgggcaagca aacattcatg cgatgacgaa gatgttctct
     2641 aaggataaat ggtcctctac ggattttacc attattcact ataccagagt ggaggatgag
     2701 cttattaaac aacaaggtgc agtaataaca actgcacaag cagaaaaaat ggctacggac
     2761 tggaaaactc tggctggttc gctggcaaca ctatttccag atcgtaataa actgtgccca
     2821 ataaaataat caacgacaac ccggcaaaac acatgtttgc cgggttaaga ttatcagacc
     2881 tcaatctccg ccatgtcgcc tttttcttgc aaccagttgc ggcgatcttc cgagcgtttc
     2941 ttcgccagca gcatatccat catcgcgtca gtacgctgat cgtcttcatc atcgatagtc
     3001 aactgcacca gacggcgagt gttcggatca agcgtggttt cgcgcaattg catcgggttc
     3061 atttccccga gacctttaaa acgctggacg tttggcttgc ctttcttgcg ttttaattgc
     3121 tcaagtacgc cctctttctc ttcttccgtc agcgcgtaat aaacctcttt cccgaggtca
     3181 atacggtaga gcggtggcag tgcaacgtaa acgtgaccgt gtttcaccaa tgcgcggaag
     3241 tgttttacaa acaaagcgca gagcagcgtg gcaatgtgca gaccatcgga gtcagcatcc
     3301 gccaggatac agatcttgcc gtaacgaagt tggctcagat cgtcactgtc aggatcgata
     3361 ccgatcgcta ccgaaatatc gtgcacttcc tgcgaagcca gcacttcgtc ggaagagact
     3421 tcccaggtgt taaggatctt acctttcagc ggcatgatcg cctgatattc gcgatcgcgc
     3481 gcctgcttgg cagatccgcc tgcggagtca ccttccacaa ggaatagttc ggtacggtta
     3541 agatcctgcg cggtacagtc agccaattta ccaggcagcg ccgggccgct ggttagcttt
     3601 ttacgcacca cttttttagc cgcacgcata cgacgctggg cgctggaaat cgccatctcc
     3661 gccagcagtt cagctgcctg aacgttctgg ttcagccaca aggtgaaggc atctttcacc
     3721 acgccagaaa caaatgccgc acactgacgc gaagagagac gttctttggt ctgcccggca
     3781 aactgcggat cctgcatttt tactgacagc acataggcgc agcgatccca gatatcttcc
     3841 gccgacagct ttacaccgcg cggcagaatg ttgcggtatt cacagaattc acgcatcgcg
     3901 tccagcaggc cctgacgcag gccattaaca tgggtaccgc cctgcatcgt tgggatcagg
     3961 ttgacgtagc tttcggtcag cagttcaccg ccttccggta gccacagtag cgcccagtcc
     4021 accgcttcag tatcgccagc gaaattaccg ataaacggtt tttctggcag cgtcggtaaa
     4081 ccgtttaccg cttccgccag gtaatcattc agaccgtcct gatagcacca gcgctgttcg
     4141 gtgttgttga tctcatcttt aaaggtgatc tcaacaccag ggcacagtac tgctttggcc
     4201 ttcagcacat gcgtcaggcg tgaaacagaa aaacgcgggc tgtcaaagaa ggtttcatcc
     4261 ggccagaagt gcacgctggt accggtattg cgcttaccgc aagtgccgac aacctgtaaa
     4321 tcctgcacct tttcgccatt ttcaaaggcg atgttataaa cctgaccatc gcggcgtacg
     4381 ttaacttcta cgcgcttcga cagggcgtta accaccgaaa tccccacgcc atgcaggccg
     4441 ccagagaact ggtaattttt gttagagaat ttaccgcctg catgcagacg gcaaagaatc
     4501 agttcaaccg ccggtacacc ctcttccggg tgaatatcca ccggcatccc gcgcccatcg
     4561 tcaatcactt ctaacgactg gtcagcatgt aagattacgt ccacgcgttt tgcgtgaccc
     4621 gccagtgctt catcgacact gttatcaata acttcttgcc ccaaatggtt agggcgagtg
     4681 gtatcggtat acatccccgg acggcggcga actggctcaa gcccggtgag tacctcaatg
     4741 gcatcagcgt tataagtttg cgtcatggtt taagttagca attcgagttg atcgtcagag
     4801 atggtgcaga ccaagaaaat cgacgatcgg gttgaaataa tcttcgaagc ccgtgaatgc
     4861 gtgattgccg ccttctatga cagtctggcg gcaggaagcg tagtacgcca ccgcctggcg
     4921 gtaatccagc acttcatctc ccgtctgttg cagcagccag atcaaatccg gcgcttccag
     4981 cgggtcaatc tgcatgactt tgagatcgta aatatggcgt gactctagca catattgctg
     5041 cccggtgtag gggttctcgt tctgaccgag atagtccgtc agcagttcaa acgggcgtac
     5101 cgccgggttt accaccactg cgggcagcat aaaacattgc gacaaccagg tggcgtaata
     5161 tccccccagt gacgaaccga caatacccag cgaatcgccg ccatgttcca ggacaatgga
     5221 ttccagcagt tctgccgcat cggaaggata cggcggcaac tgcgggatga tcatctcaac
     5281 gtcagggtga tgttccgcaa gccagttttt taacaagctc gcttttgcag agcgcggcga
     5341 gctgttgaaa ccgtgtaaat aaagaagcgt agacatcagt agccttctga agcggtatca
     5401 ggttggaaac gtgtgtccgc caggcgatgc acctcggtgg tcagcgtgcc atcagcatgt
     5461 aactcgagag tacgccagcc gggcgcgatg gtgtccagcg taaagttgga acagtgcggc
     5521 ttaaactgca cacaggtcga cggcgttgcc agcaggcggc gaccgttcca gtcgagatcc
     5581 agctcctgat gaatatgacc gcacagcaag tatttgacgt gcggaaactt cgccagcacg
     5641 gtatccagtt cgcccgcgtt acgcaggctg tgttgatcaa gccaactaca acccgcaggt
     5701 agcggatgat gatgcagcag cagcaacgta tggcgttctg gcgcatcggc cagtttacgt
     5761 tccagccact caagctgaaa ctcgctcagc tcaccgtgcg gcacgccaaa cacctggcta
     5821 tccagcaaca ggatttgcca ttgctcacca ataaacaccc gcttcgccgg ggagataccc
     5881 gcatcctgta acgcgctgta catcgcgggc tggaaatcgt ggttgcccgg cagccagacg
     5941 cagggcgcac gaaaacttgc gatgccttca gcgaaatgct gataggccgc agaggattga
     6001 tcctgcgcta aatcacctgt cgcgacaatc aggtcgaatt cgtgctggtg tggacgaatc
     6061 gcctccagca ccgcctggta gctttcccag gtgtttaccc ctaacagggc ttcgtgcttt
     6121 tgtgcaaaca ggtgagtgtc ggtaatttgt aaaatcctga ctctggcctc accagccaga
     6181 ggaagggtta acaggctttc caaatggtgt ccttaggttt cacgacgcta gtaaaccgga
     6241 atcgccatcg ctccatgtgc taaacagtat cgcaaccagt ccgctaaaaa ctggttaatt
     6301 tgatgctttt cgtcgcgttg atgcaacttt ttattaggat aatcataccg cgctttgaag
     6361 cgaaaaatct gctggcttga acacacttca gccaccatcg cgtcatgata cagacgcacc
     6421 gtcattgacg gaaggctcca gtaactgatc gcgggcgcag tctgttctat tgtcaccagg
     6481 gtagtgtatc gggtcgattc cacaatcgtc agccgatatt gtgcgtttgc cacctgatag
     6541 cttacagttt cgccgggtgc gtcattgcgc ggtaacaaac ggcgcaattg tgaaaagttc
     6601 atctcgcaca ggcgcatcat ttcaggaaag tcaggtgtgt aacgcttcat ttatgcccac
     6661 tcatttttta acgcttgatg atgcagctgc agccattgca aagcgatgac cgacgctgcg
     6721 ttgtcgattt tcccctcttc tacccactgg tatgcctgtt cccggcttac cacatgaacg
     6781 cgaatatctt cgttttcatc agccagaccg tgaataccgc ttgcggtcgt ggcgtccact
     6841 tcgcccacca taattgacga acgctcactg gtgccccccg ggcttgccag gaaacttaac
     6901 accggtttgg tccgtttgac tatcagtccc gcctcttcaa tcgcttcgcg acgggcaaca
     6961 tcttccacac tttcaccctc ttcaatcatc ccggcaacca tctccagtag ccagggggtt
     7021 tcgctagtgt cgtacgcggc aattcgaatc tgctcaatca gcacaacttc atcacgcact
     7081 gggtcaaagg gtagcaagac tgcggcgtga ccgcgctcaa aaatttcccg ccgtacctca
     7141 tgactcattt gcccgttgaa tagacgatga cgaaatctat aaagatctaa tgaaaaaaag
     7201 ccgcgataaa gtgtttctcg tgcaataatt tctacatcgt ttttgccaaa tgtaacgggc
     7261 aggttgtctg gcttaagcat tgttaatgtc ctggcactaa tagtgaatta aatgtgaatt
     7321 tcagcgacgt ttgactgccg tttgagcagt catgtgttaa attgaggcac attaacgccc
     7381 tatggcacgt aacgccaacc ttttgcggta gcggcttctg ctagaatccg caataatttt
     7441 acagtttgat cgcgctaaat actgcttcac cacaaggaat gcaaatgaag aaattgctcc
     7501 ccattcttat cggcctgagc ctttctggtt tcagttcgtt gagccaggcc gagaacctga
     7561 tgcaagttta tcagcaagca cgccttagta acccggaatt gcgtaagtct gccgccgatc
     7621 gtgatgctgc ctttgaaaaa attaatgaag cgcgcagtcc attactgcca cagctaggtt
     7681 taggtgcaga ttacacctat agcaacggct accgcgacgc gaacggcatc aactctaacg
     7741 cgaccagtgc gtccctgcag ttaactcaat ccatttttga tatgtcgaaa tggcgtgcgt
     7801 taacgctgca ggaaaaagca gctgggattc aggacgtcac gtatcagacc gatcaacaaa
     7861 ccttgatcct caacaccgcg accgcttatt tcaacgtgtt gaatgctatt gacgttcttt
     7921 cctatacaca ggcgcaaaaa gaagcgatct accgtcaatt agatcaaacc acccaacgtt
     7981 ttaacgtggg cctggtagcg atcactgacg tgcagaacgc ccgcgcacag tacgataccg
     8041 tgctggcgaa cgaagtgacc gcacgtaata accttgataa cgcggtagag cagctgcgcc
     8101 agatcaccgg taactactat ccagaactgg cggcgctgaa tgtcgaaaac tttaaaaccg
     8161 acaaaccaca gccggttaac acgctgctga aagaagccga aaaacgcaac ctgtcgctgt
     8221 tacaggcgcg cttgagccag gacctggcgc gcgagcaaat tcgccaggcg caggatggtc
     8281 acttaccgac tctggattta acggcttcta ccgggatttc tgacacctct tatagcggtt
     8341 cgaaaacccg tggtgccgct ggtacccagt atgacgacag caatatgggc cagaacaaag
     8401 ttggcctgag cttctcgctg ccgatttatc agggcggaat ggttaactcg caggtgaaac
     8461 aggcccagta caactttgtt ggtgccagcg agcaactgga aagcgcgcat cgtagcgtcg
     8521 tgcaaaccgt acgttcctcc ttcaacaaca ttaatgcttc tatcagtagc attaacgcct
     8581 acaaacaagc cgtagtttcc gctcaaagct cattagacgc gatggaagcg ggttactcgg
     8641 tcggtacgcg taccattgtt gatgtgttgg atgcgaccac cacgctgtac aacgccaagc
     8701 aagagctggc gaatgcgcgt tataactacc tgattaacca gttgaatatt aaatcagctc
     8761 tgggtacgtt gaacgagcag gatcttctgg cactgaacaa tgcgctgagc aaaccggttt
     8821 ccactaatcc ggaaaacgtt gccccgcaaa cgccggaaca gaatgctatt gctgatggtt
     8881 atgcgcctga tagcccggca cccgtcgttc agcaaacatc cgcacgcact accaccagta
     8941 acggtcataa ccctttccgt aactgatgac gacgacgggg cttcggcccc gtctgaacgt
     9001 aaggcaacgt aaagatacgg gttatctgcc gcattcttcc cccttctcgc ttcaatttcg
     9061 accagccatc ctctattctg atgggtattt accactggtc ccggaagaca aaaatgaaac
     9121 ggacaaaatc catacgccac gcatcgttcc gcaaaaactg gagcgcacgc catctgacac
     9181 cagtcgctct cgcggttgcc actgttttta tgctggctgg ctgtgaaaag agtgatgaaa
     9241 cagtgtctct ctatcaaaat gctgacgact gttcagctgc aaacccaggc aaaagcgccg
     9301 aatgtaccac cgcgtacaac aatgcgctga aagaagccga acgtactgcg ccgaaatacg
     9361 ccactcgtga agactgtatt gctgaatttg gcgaaggtca gtgccagcag gcaccagccc
     9421 aggctggcat ggcaccggaa aaccaggcgc aggcccagca atccagcggg agtttctgga
     9481 tgccgctgat ggccggttac atgatgggtc gtctgatggg cggcggtgcg ggatttgcac
     9541 agcagccgct gttctcctcg aaaaacccag ccagcccggc ttacggtaaa tataccgacg
     9601 cgacgggtaa aaactatggc gcagcccagc caggccgcac catgaccgta ccgaagacag
     9661 ccatggcacc aaaaccggcg accaccacta ccgttacccg tggcggtttt ggtgaatctg
     9721 ttgccaaaca aagcactatg cagcgtagcg caaccggcac ctcttctcgt tcaatgggtg
     9781 gctgataccg atggaaagag tcagtattac cgagcgcccg gactggcgtg agaaagccca
     9841 cgaatacggt ttcaattttc acaccatgta cggcgagccg tactggtgtg aagatgctta
     9901 ctacaagttg accctcgccc aggttgaaaa gctggaagaa gtcaccgccg aactgcacca
     9961 gatgtgcctg aaagtggtgg aaaaagtgat cgccagcgat gagctgatga ccaaattccg
    10021 cattccaaaa cacacctgga gttttgtgcg tcagtcatgg ctgactcacc agccatcgct
    10081 ttattcgcgt cttgatctgg cgtgggatgg cactggcgaa cctaaactgc tggaaaataa
    10141 cgccgataca ccaacgtcac tatacgaggc ggcgttcttt cagtggatct ggctggaaga
    10201 tcagcttaac gccggtaact tgccggaggg cagcgaccag tttaacagtc tgcaagaaaa
    10261 gctaatcgat cgctttgtcg agttgcgtga acagtatggc ttccagttgc tgcatctcac
    10321 ctgctgtcgc gacacggtgg aagatcgcgg aaccattcag tatttgcagg actgcgcgac
    10381 ggaagctgaa atcgctaccg aattcctcta catcgacgat atcggtttag gcgaaaaagg
    10441 tcagttcacg gatttacagg atcaggtaat ttccaacctg ttcaagctgt atccgtggga
    10501 atttatgctg cgcgagatgt tctccaccaa gctggaggat gcaggcgtac gctggctgga
    10561 accggcgtgg aagagcatta tctccaacaa agcgcttctg ccgctactgt gggagatgtt
    10621 cccgaatcac ccgaacctgc tgcctgctta ttttgcggaa gatgatcatc cgcaaatgga
    10681 aaaatatgtg gttaaaccga tcttctcccg tgaaggcgca aacgtgtcga tcattgagaa
    10741 cggcaaaacc attgaagcag cggaaggtcc gtatggcgaa gaagggatga ttgttcagca
    10801 attccacccg ttaccgaaat tcggcgacag ctatatgctg attggtagct ggctggtgaa
    10861 cgatcaacct gccggaattg gcattcgtga agaccgtgca ttgatcaccc aggatatgtc
    10921 tcggttttat ccgcatattt ttgttgagta atacagctta atattgcccc gataagcggg
    10981 gcaatctctt ataatccacc attcataatt cccatcaata tataaacaca tacatatcca
    11041 acctttattt aacattaata ctcaatttaa tgttaaatcg tcatatgcaa gcgatattgt
    11101 aattaaagta agtgaataca tcacaacata actatttgat attatttaaa tactttacgt
    11161 tcatttcacc agatttaaat ataataatta aaaaacaatg ttgcagggat cacactacac
    11221 agtattgaac attgtgattg atatattgcc cccctactat ccattaaaca taaatatttg
    11281 tttcaccgaa taacaacata tttaaaagaa acacaaaagc attctcactc tttattgaat
    11341 taatatttat attcacgtaa cgaatttatt agcaccctct caacagtcga tattcatcat
    11401 tattatatat ttgcgttata taaggagata tatccatgtt tgataaatat agaaaaacac
    11461 tcgtagccgg aactgtggcg ataaccctgg gtttgtcagc atcgggggtg atggctgcgg
    11521 gttttaaacc agcgccgcct gccgggcaac tgggtgcggt cattgtcgat ccctacggca
    11581 atgcaccact gaccgctttg gttgacttag atagccatgt tatttctgac gtcaaagtta
    11641 ccgtccatgg gaagggcgaa aaaggtgtag aaatcagcta tcccgtgggt caggaatcac
    11701 taaaaactta cgatggtgta ccgatttttg gtctttatca gaaatttgct aacaaagtga
    11761 ccgttgagtg gaaagaaaac ggcaaggtca tgaaagatga ttatgtggtg cacacttcgg
    11821 ccatcgtcaa taattacatg gataaccgct ctatatccga tttacaacag accaaagtta
    11881 ttaaagtcgc accgggtttt gaagatcgcc tctatctggt taatacccac acctttaccg
    11941 cccaaggttc cgatctccac tggcatggtg agaaagataa aaatgccggt atccttgatg
    12001 cgggtccggc aaccggcgca ctaccttttg atatcgcgcc ctttactttc atcgtcgata
    12061 ccgaaggtga ataccgctgg tggctggatc aagacacttt ctacgatggt cgtgaccgcg
    12121 atatcaacaa gcgtggttat ctgatgggta tccgcgaaac gccacgcggc acctttaccg
    12181 ctgtacaagg tcagcactgg tacgagttcg acatgatggg gcaggtgctc gaagatcaca
    12241 aactaccgcg cggatttgct gacgccactc atgagtccat tgagacgcca aatggcacgg
    12301 tactgttgcg cgtaggtaag agtaactatc gtcgcgatga cggcgtacac gtcaccacca
    12361 ttcgtgacca tatcctcgaa gttgataaat ctggtcgcgt tgtcgatgta tgggatctga
    12421 ccaagatcct cgatccgaaa cgcgatgcac tgctcggcgc gctggatgca ggtgcagttt
    12481 gcgttaacgt tgaccttgcc catgcaggac aacaggcaaa actggaacca gatacaccgt
    12541 tcggcgacgc gctgggtgta gggccaggcc gcaactgggc gcacgtgaac tctatcgcct
    12601 atgacgcaaa agatgactct atcatcctct cctctcgcca ccagggtgtt gtgaaaattg
    12661 gtcgtgataa gcaagtgaaa tggatccttg cgccctctaa aggttgggaa aaaccgctgg
    12721 ccagcaagct gctgaaaccg gttgatgcta acggtaagcc aattacctgt aacgaaaatg
    12781 gcctgtgcga aaactcagac ttcgacttta cctacaccca gcataccgcc tggatttcca
    12841 gcaaaggaac gctcaccatt tttgataatg gcgatgggcg tcatctggaa caacctgcct
    12901 taccaaccat gaaatactcc cgctttgtgg aatataagat tgatgagaag aaaggcaccg
    12961 ttcagcaagt gtgggaatac ggtaaagaac gtggctacga tttctatagc ccgattacct
    13021 ccatcattga atatcaagcc gaccgtaaca ccatgtttgg cttcggtggt tctattcatt
    13081 tgttcgatgt cgggcagcca accgtcggta agttgaacga aatcgattac aaaaccaaag
    13141 aagtgaaagt ggaaatcgac gtgctgtcag ataaacccaa tcagactcac tatcgtgcac
    13201 tgttagtccg tccacaacag atgttcaaat aattaagaag ggaataaaaa tgtcgaaatt
    13261 agggatctca tcactattta aaaccatact gcttaccgca gctttggctg tttcatttac
    13321 cgcttctgca tttaccgaag gcacggatta catggttctg gaaaaaccta ttccgaatgc
    13381 cgacaaaacg ctgattaagg tattcagcta cgcctgcccg ttctgttaca agtacgacaa
    13441 agcggttacc ggtccggtat cggaaaaagt gaaagatatc gttgctttca cgccgttcca
    13501 tctggaaacc aaaggtgaat acggcaaaca agcaagcgaa gtctttgcgg tcttgatcaa
    13561 taaagataaa gcagcaggca tttccttatt tgatgctaac tctcagttta agaaagccaa
    13621 gtttgcctac tacgcggctt atcacgacaa aaaagaacgc tggtctgacg gtaaagatcc
    13681 ggctgctttt atcaaaaccg gtctggatgc cgcaggtatg agcttggcgg attttgaagc
    13741 tgcactgaaa gaaccagcgg ttcaagaaac actggaaaaa tggaaagcct cttatgatgt
    13801 cgccaaaatc cagggggttc cagcctatgt cgttaatggt aagtacttga tctacaccaa
    13861 gagcatcaaa tccatcgacg cgatggcaga ccttatccgt gagttagcaa gcaaataaca
    13921 gggaaacagg ctatgggtat taagggaatg tggaaagacc ttcgcacatc accagttgac
    13981 acactggtca gatggcagga gcaacgcttg ttgtggttgt taatggccgt tgcgatgggc
    14041 gcgttaatta tcctggcgca ctctttcttc cagatctatc tctacatggc accctgtgag
    14101 caatgtgtat acattcgcta cgccatgttt gtgatggtta ttggaggatt ggttgcagcg
    14161 atcaacccaa agaacatcat cctaaagcta attggctgcg tgatggcatt ttacggcagc
    14221 attttgggac tgaagttttc gctcaaacta aacgatatcc accatgcagt acataatccg
    14281 gatcccgatt cactgtttgg cgtacagggt tgctctaccg atccaacttt tccctttaac
    14341 ctgccgctgg cacagtgggc accaaattgg ttcaagccta caggggattg tggctatgac
    14401 gcaccaatcg ttcctgatgg cgtcacactg agcagcacgc aacagtggtt tgtggaaatg
    14461 tatcagcaat ctgaaggctg gtatctgcta ccaccatggc attttatgaa tatggctcag
    14521 gcttgtatgc tggcttttgg tatgtgtctg gtgctgctgg tcattatgag tggcgcatgg
    14581 gcgcttaaaa tcgtcagagg ctaactcagc aacacccgaa gccagtcgtt ctggcttcgg
    14641 gttcttttat tagcctatct gcaccgacaa catacttaaa ctgcccattt ctataccatc
    14701 gaccggaatg gtaattggct cctgcccatc ccacgcacct aagacataca acaacggcag
    14761 atagtgctct ggcgttgggt tcgataacgc gccaccttca tggtcgagat aattcaccag
    14821 aggatgctgc tctaccggcc cttgccacgt cagattcgct ttcacatact cattaaacga
    14881 cgtcgcccac ggatacggtg aactatcacc gtgccacttc actgtgcgca ggttatgcac
    14941 cacgttaccg ctggcgacca acattattcc ttcatctcgc agcgccgcga gtttgcgccc
    15001 catttcgaaa tgccaggcgg caggtttgct actgtcgata ctcaactgca ccatcgggat
    15061 atccgcgtca ggatacatct taatcagcac gccccaggaa ccgtggtcaa agccccaggc
    15121 ttctttatcc agcgtcaccg ggatcggcgc taacagctca accagacgct gtgccagcgc
    15181 aggcgaaccc ggagcaggat aatgcgtatc gtacagcgcc tgcgggaagc caccaaagtc
    15241 atgaatcgtg ggcggcgtct ccatcgcggt cactcctgtt ccacgggtaa accagtgagc
    15301 cgaaaccacc acaatcgctt gcgggcgtgg caatgtcatc cccaacgtct gccagctgcg
    15361 ggtatacaaa ttatcttcca gcacgttcat cggactaccg tgacctaaaa acaatgctgg
    15421 catacgtgtt gaagacatga tgatatcctt aactaaaggt gtcattttga tatcctcaca
    15481 atacgcttgt tcggcggagt aagaacccgg ataacaatga tgatgatcat cagttatttt
    15541 gacgatctgc ctgaaggtga agatttataa ggagttgtcg atgtcagtac ctctcattct
    15601 gaccatactg gcgggggcag ccacgtttat tggcgcgttt ctcggcgttc tcgggcaaaa
    15661 accctcgaac cgcttactgg cgttttcgct aggatttgcg gcggggatca tgttgctcat
    15721 ctcattaatg gaaatgcttc ctgccgcact agccgccgaa ggaatgtcgc ctgtgttggg
    15781 ttatggaatg tttatcttcg gtctgcttgg ctattttggc ctggaccgca tgttgccaca
    15841 tgcgcatccg caggatttaa tgcaaaaatc ggtgcagccg ttgccaaaat cgatcaagcg
    15901 cacagccatt ctgctcactc tcggcatcag tctgcataac ttcccggaag ggattgccac
    15961 ctttgtcacg gcgagcagca acctggagct gggctttggc atcgcactgg ccgttgcgtt
    16021 acacaatatt cctgaaggtc tggcggttgc aggcccggtt tacgcggcaa cggggtctaa
    16081 acgcaccgca attctttggg cggggatttc tggactggca gaaattcttg gtggtgtgct
    16141 ggcgtggtta atcctcggta gcatgatttc cccggtggtc atggcggcaa tcatggcggc
    16201 ggttgcagga attatggtgg cgctctcggt tgatgaatta atgccgctcg ccaaagagat
    16261 tgaccctaat aataacccca gctatggcgt actatgtgga atgtcagtga tgggattcag
    16321 tttagtgctg ctacaaacgg cgggaattgg ttaaaagcaa catcagaagt tgtccagcgc
    16381 atggacaact tctgccagct tcactaaagc cttattctac cttttcggct gtctcttctc
    16441 tcgtactgtt taagtatttg ttccgcgctt caagcagtgc gtctctgtcc tggcgaaacg
    16501 cccgacaata acgtttcaaa tgcaggaaat aacccaccac aacaagagca atcaatacaa
    16561 tccagtacca ggcgataaac atccttttac ccttaatatt taacaatact aaacatttag
    16621 cgcataaatt tcacatatcc tttttcggat atattcatat ggtcgcataa taacggaaca
    16681 attatatgat tttgatcata atgaaaaaga atgcctgata tcaatgaaat tttctcatag
    16741 aaagagaagc ggaattaaca tcttgcatag cagggcaaaa taaaaaccga agccagctac
    16801 aacttcggtt tcttgattaa gacagtaaat taagcagcga ttttcagctg gctttgcgtt
    16861 catgcgcctg acgataagcc accaggtctt caatagtcac gagcgccata ttgtgtttat
    16921 tggcaaactc aatgcactct ggtgcacgcg ccatcgtgcc atcgtcatta gtcagctcac
    16981 acagcacacc agccggttta aagccagcca gcgtcatcag atcaatagtc gcttcagtat
    17041 ggccgccacg cgtcagtaca ccaccagcct gagcgcgaag tgggaaaacg tggcccgggc
    17101 gattcagatc agaaggtttt gcgccatcgg caatcgctgc gcgaacggtc gtaatacggt
    17161 cagcggcaga aacaccggta gtcacacctt cagctgcttc aatggtcacg gtaaaaccgg
    17221 tgccataggc gctggtgtta ttttctacca tcattggcag atcgagttgt ttacggcgat
    17281 cttcagtgat gcacaggcaa acaataccgc taccgtggcg aatggtcagc gccatctgct
    17341 caacggtcat ggtttctgcc gggaagatca tatcaccttc gttttcacgg tcttcatcat
    17401 caagcaccat tacaccgcgt ccttcacgca gcgcaaccag tgcattttca acacgttcga
    17461 aaggcgtacc aaaagaggaa agtagcgtct gattcatggt aaaaaaacct cactaaaatt
    17521 atggttacca gaatcagggc agtcttagga gtggcggcat atagccaaaa taacgtgagc
    17581 gggtccatgc ccgacagaat cgttactctc tcccatccgg actctaaccg tcggccccgg
    17641 aattacaccg gatctgctgt cctttgagtt cgcacccaaa gcgctcgcgg gctttcaact
    17701 gagttgattt accgccggtg gggaatttcg ccccgccctg agaataagcg gattcactat
    17761 aacgctaatg attagcggca gcaacgcata gcttcacata attctggttt atgacttacc
    17821 cttatcgcac tacaatggca ctcaacacct atcagtacag ggaaactaca atgattgacc
    17881 cgaaaaaaat tgagcaaatc gctcgccagg ttcacgaatc aatgcctaaa ggaatcaggg
    17941 agttcgggga agatgtggag aaaaaaatcc gccaaaccct acaagcgcag ctgacgcgtc
    18001 tcgatctggt aagccgcgaa gaattcgacg ttcagacgca agtcctgtta cggactcgtg
    18061 aaaaactggc gttactggag cagcgcatca gtgaactgga agcgcgcaat aattcggtag
    18121 cagatcttca atctcctccg gctatcccgc ctattgataa agcagaataa agcatcacaa
    18181 aaacgggcca gacggcccgt aatatcagtg ttctaattca agttctctgg tttgcatcat
    18241 ctgttggcaa taccaggcat aacgtgcgca aaaccaggtt ctatgttctt catccatgtt
    18301 accagtaacg gccagaatat cgaccgggcg accttctgcg tgcattctgg caaaactacg
    18361 cgccaggaaa tcgaaattat ttaaagaatt aagactatga tccataagca catcctccgg
    18421 ttatcgcata ccttttaagg ccatatgctt tttctgattt cagtattccc caatcagtgt
    18481 agtgccttta aacataacgt gcgtaaatat attgtccccg atcacacttt ttagtgaaaa
    18541 ataaaaaacc atattaaaag actttctctt ccagaacttc tctaacatgt gttattatta
    18601 acaaagataa atgtaagcaa ggaaactatc ctaaagtaaa tattgcgtca ctaaatggac
    18661 atcggagtga catatgattt tattcgccga ctataacacc ccttatcttt tcgctatttc
    18721 ctttgtactg ttgatcggcc ttttagaaat ttttgcgctt atctgcggtc acatgctctc
    18781 cggtgctctt gatgcacatc ttgaccatta tgattccata accacaggtc atattagcca
    18841 ggcacttcat tatctcaata tcggaagatt gccagctctc gtcgttctct gcttactggc
    18901 tggttttttt ggtcttattg gcatcctgtt gcagcatacc tgcctcatgg tctggcaatc
    18961 accactctcg aacctgtttg tggttcctgt cagtttactg tttacgatta ttgcggtgca
    19021 ttataccggt aaaatagttg cgccctggat tccacgcgat cacagctcag ctattacaga
    19081 agaagaatat attggtagca tggcgttaat taccggtcat caagccactt caggtaatcc
    19141 ctgcgaagga aaactcaccg atcaatttgg ccaaatacat tatttactac tggagcctga
    19201 agaaggaaaa attttcacta aaggagacaa agtgttaatt atttgccgac tttctgcaac
    19261 tcgatatttg gcggaaaaca atccctggcc ccaaatattg taatttatta atcaaaagga
    19321 aatttaataa tggatgatat tgttaattct gtgccctcct ggatgtttac cgcgattatt
    19381 gccgtatgca ttctgtttat tattggaatt attttcgcca ggctctatcg tcgcgcttcg
    19441 gcagagcaag cttttgttcg tactggttta ggtgggcaaa aagtggtaat gagcggtggc
    19501 gcaatcgtga tgccgatctt tcatgaaata atccccatca atatgaatac tctgaagctg
    19561 gaagtcagcc gctcaaccat tgatagcctg attacgaaag atcgtatgcg cgtcgatgtc
    19621 gttgtcgctt tctttgtgcg ggtaaaaccg tcagtggaag gtattgccac cgccgcccag
    19681 acgcttgggc aacgcaccct gtcgcctgaa gacttacgta tgttggttga agataaattt
    19741 gtcgatgccc tccgtgcaac agctgcacaa atgaccatgc atgagttaca ggatacccgc
    19801 gagaacttcg tgcagggcgt acaaaatacc gtagctgaag atctgtcgaa aaacggcctg
    19861 gaactggaaa gcgtttcact taccaacttt aaccagacgt cgaaagaaca tttcaatcct
    19921 aacaacgcct ttgacgccga aggtttaact aagctgacgc aggagacgga gcgccgtcgt
    19981 cgcgaacgta acgaagttga acaggatgta gaagttgcgg tgcgtgagaa aaaccgtgat
    20041 gcgctttcgc ggaagctgga gattgaacag caagaagcgt ttatgacgct tgagcaggag
    20101 cagcaggtta aaacccgtac cgctgagcag aatgcgaaaa ttgcggcttt tgaggccgag
    20161 cgtcgtcggg aagcagagca gacgcgaatt ctggctgaac gacagattca ggaaacggaa
    20221 atcgaccgcg aacaggccgt ccgttccaga aaagttgaag ctgaacgtga agttcgcatt
    20281 aaagagatcg aacagcagca ggtcaccgaa atcgccaacc agacgaaatc gatcgcgatt
    20341 gccgccaaat cggaacagca gtcacaagcg gaagcgcgtg ccaaccttgc acttgcagaa
    20401 gcggtaagcg cccagcaaaa cgtagaaacc actcgccaga ctgccgaagc cgatcgtgct
    20461 aaacaagtgg cgttgatcgc cgcagcgcag gatgcagaaa ccaaagcggt tgaactgacc
    20521 gtgcgggcga aagcagaaaa agaagccgca gaaatgcagg cggcggctat cgttgagtta
    20581 gccgaagcta cacgtaaaaa gggtctggcg gaagcagaag cacaacgtgc gctgaacgat
    20641 gctatcaacg tactttctga tgaacaaacc agccttaaat tcaaactggc attattgcag
    20701 gcgctgcctg cggtaattga gaaatccgtg gaaccaatga agtctatcga cggcatcaaa
    20761 attattcagg tcgatgggct gaatcgtggt ggcgctacgg gtgatgcaaa cacgggtaat
    20821 gtggggggcg gaaacctggc agaacaagcg ttatcagccg cactctctta ccgtacacag
    20881 gcaccgctga ttgactcctt gctcaatgaa attggcgttt caggcggctc actggcggca
    20941 ttgacttcat ccttaagctc aacaactccc gtcgaagaaa aagcagagta aaccctcttc
    21001 tatgaataat ccctggccca aaaagccggg gattttttat atctgcgttc cgctaaaagg
    21061 tgcaaatgat caagctgttg cagcattttg cgtgactgct cggggaaggc aaaactgcct
    21121 ctgggaaagc gttgcgcggg gtccggcgct catcaacaat cggggagtca gcaaggggct
    21181 gaaacgggaa agcccctccc gaggaagggg ccttgtataa ggaaagggtt atgatgaagc
    21241 tcgtcatcat actgattgtg ttgttactcg taagtttcgc agcttattaa cagccaatca
    21301 gaggggggag aaatcctccc tcacccttgt tcctttactt taggttgaaa aaaaatcagc
    21361 gtcaataggc ccgcctgcta caaagcgaga tctgtgaacc gctttccggt tagccttttt
    21421 tatcctgttg gatcttcttg atgatgttgg tcgtcgagca accgtcttca aagttgagca
    21481 ccagcacttc accaccgttg gcccagactt ctttactccc ggcaatctct tctggtttat
    21541 agtcgccgcc tttcaccagt agatctggca agataccggc gatcaagcgc tgcggcgtgt
    21601 cctcttcaaa cgacaccacc cagtcgaccg cttccagtgc gcccagcaca atcatacgct
    21661 gttcgagctg gttaacgggg cgggaatccc ctttcagccg tttggtggag gcatcgctgt
    21721 tcacagcgac aatcaaacgg tcacccagtt tacgggcatt tgccagataa gagacgtgcc
    21781 cagcgtgcag gatgtcaaag acaccgttgg tcatcaccac tttttcaccg cgtttacgcg
    21841 ctgccgctac agcaagcttc agttcctctt cggtcatcac accaaagccg gtatctgcac
    21901 gtccacgtac agcgttttcc agctcgatcg gcgaaaccgt ggatgtcccc agtttgccga
    21961 ccaccacacc agccgccgca ttggcaaaga agcaggcttc ttccagtgaa ttacccgctg
    22021 ccagcgttgc cgccaggacg ccaatcaccg tgtcgcccgc accggtaacg tcatacactt
    22081 cctgcgcctg ggttggcata tgcagtggcg ctttacccgg ttgcagcagc gacataccct
    22141 gttcggaacg ggtcactaac agagccgaga gttcgtaatc ggcaatcagt ttcatgccgc
    22201 gctcaacaat ctcttcttcg gtcttacatt taccgacaac agcttcaaat tccgagagat
    22261 tcggcgttaa cagcgtagcg ccgcggtagc gctcaaaatc ggtacctttt ggatcaatca
    22321 gcaccggaac acccgcttta cgcgccagtt ggatcatctg ctgtacgctt gccagcgcac
    22381 ctttggcgta atcagaaagc accagcgcgc caatcgaact cagtgcctga ttaatccgct
    22441 cgtgcagcgg ctgcggatca accccttcga aaccttcttc gaaatccaga cggatcagtt
    22501 gttggttgcg ggaaagtacc cgtaatttgg tgatggtcgg atgcgtcggt acagaaacaa
    22561 agtcgcattt gacgttgacg tcggccagag atttactgag cgcgcgcgcc gcatcgtcaa
    22621 tgcccgtcaa cccgaccagg cgtgcattag caccgagaga agcgatattc atcgccacgt
    22681 tagccgcgcc gcccggacgt tcttcgatgg tattcacttt aaccacgggc accggcgctt
    22741 ccggcgagat acggctggtg ggaccgtacc agtaacgatc cagcatcaca tcaccaacca
    22801 ccatcactcc tgcacgttca aactctggca gcgttacttt cattcctgtc tcctgagaga
    22861 ttcaaaattt gcgcgcgata ataccatact tcattcttcc accagccact tctgccagct
    22921 tgcccgcacc agttcacgct ctgcggtgaa gcaatcctcc gacacatgac ccggcaattc
    22981 ctgtaatgcc agatgatgaa gttcatcgcg cagcgtcgtg taagcacggg tcagcgccat
    23041 cgcttcctgc tcttccataa tgtcgttttg cgccagtagt tccagaatgc gcacgttgtc
    23101 tgaccagcgc gttaacttcg gtttttcgtg agcgtagcgc aacaccagat attgagtaat
    23161 aaattcgata tcggtaattc cgccttcatc ggctttgata tcaaagcgat cgcgatgttt
    23221 attgccgaga tgggcacgca ttttctcgcg catttcccgc acttctgttt gcagggtttt
    23281 gccttcgcgc ggcagcgtca taatctcccg gcgtactgcg tcaaagtgcg cggtgagctg
    23341 cggatcgccg tacactacac gcgcacgcac cagcgcctga tgctcccacg tccaggcctc
    23401 gtttttctga taatcagcaa atgcttctgc ggatgtcacc agcattcccg ccgccccgga
    23461 cggacgcagt cgtgcatcca cttcatacaa aatgccggaa gaggttcgcg tactgaacag
    23521 atgcataatg cgttgcgcca gacgcagata aaactgtcgc ccgtcgattt cccgctcgcc
    23581 gtcagtcatc gcatccattg ggcaatcatg gaggaagatc aggtcaagat cggaactgta
    23641 gcctaactcc cagccaccca gcttgccgta gccgaccacc gcaaaaccac gcccttcgcg
    23701 ttcgttcagg tgatttggct taccgtagcg ggcaaccatt tgaacccacg cctgctgaac
    23761 gacggcatca atcatggctt ctgccagcca ggttaagtga tcgctcactt tcattaccgg
    23821 tagcgtaccg gcgatatccg ctgccgcaat gcgtaacagc tgcgcctgtt tgaactgacg
    23881 cagcgcctca agctgttgtt cttcgtcatc ttccggcacg cgcagcaaat actggcgcag
    23941 ttcatcacgg taggcatccg tcgccgttgg ttggtagagg gtatttggat cgagcaattc
    24001 atccagcaat aatggataac gcgctagctg gctggcaatc atcggcgatg cagcacacag
    24061 agaaatcaaa tgtttaagcg ccgcagggaa ttcactaagc agctcaagat aagtggtgcg
    24121 agtaacaatc cccaccagca aggcggtaat gcgcgacagc gtgacagcag cgtcttcacg
    24181 cgcacagaca tcacttagca gatgtggcat cagatggtcg agcacctgac gtcctcgcgg
    24241 cccgatggtg cgcttatcca gctctttgcg gaaatcagca atcagcatta gcacctgttt
    24301 gcgatcatcc tcgctaagat gcgccagcac tggcgtggtg tcatcttcct gcaacgcatc
    24361 ctgccacagc tcacgccact gttccgacag cgactcttcc tgagtttcac tttcgtcgtc
    24421 gccaatcaat tcattaaaca cccggcgcac attggccata tgtgcggtca gcaccccggt
    24481 caattgcggc cagtcagcaa agtccatcgc ccacgccagc cgcgcacggt taagctcatc
    24541 aaaaggaagc gtctgggttt gttcgtcgtt aatgctttgc agcaggtttt ccagacgccg
    24601 caggaacaga tacgccactc gcaattgttc agcatcgttt tcagaaagca gatgcagcgc
    24661 ggcaatggcg ctgagcgttg gcagtaaaga gcgcgattgc agcgacggtt cgcgcccgcc
    24721 gcgaatgagc tggaacacct ggacgataaa ttcaatttcg cgaatgccgc ctgcgccaag
    24781 tttgatgttg tcggtcaaac cgcgtcgacg cacttcacgg gcaatcattc ctttcatgtt
    24841 gcgcagcgac tgaatcacgc tgaaatcgat gtaacgacgg aaaacaaacg gacgcagcat
    24901 cgcacgcaac tcgttagcat agacgccgtc gctatccccc ataatccgcg ccttgaccat
    24961 cgcgtaacgc tcccagtcgc gcccctgctc ctggtaataa tcttccagcg ccgcaaaact
    25021 cagcaccagc gggccacttt caccaaacgg acgcagccgc atatccacgc gatagacgaa
    25081 accatcctgc gttggttgat ccagcacttt aatcagccgc tgccccatgc gggtaaaaaa
    25141 ctgcgcgtta tccagttccc ggcgtccgcc ctgggtacaa ccatgttccg gccaggcaaa
    25201 aatcagatcg atatctgagg agaaattcag ttccccaccg cccagcttac ccatgcctaa
    25261 aatcagcagc ggttgcgctt cgccctgcgc attgcacggc gttccccatt cgcggcagca
    25321 ggcgtcatac agccagtcac gcgccgcaac aatcagcgtc tccgccagat ggctgagctg
    25381 ctgcaatatg ctctcttcag taaccagtgc cagcgtttgc gcccaggcga tgcgcaccat
    25441 aatgcgccgc cggaatagcc gcagctcgcg cattaacccg gcttcgtcac tcacattact
    25501 gagcgcctcc tgcaaccatg ctgcgtaatg ctgccattcg tcggcctgcg gcggttggct
    25561 ttccagttcc gtcagccact ctggatgcgc aattatgctg tcctgcagaa aatcactgaa
    25621 agtaagtact gacttcgcct gtgcgctaag tgattcctcg gctaaaggct ctggcagccg
    25681 ctcaacaacg gtctgccagt actgctgtaa cggtgaagag agcggcttca taagcgattt
    25741 tatccttgcc tgattaacgt tttccgctgt gcaaccagaa cggttcctga ttgtttgcct
    25801 cattacggaa atgttcaatt tcaatgcgtt gcccggtcgc aatagcgtga cgcagcccct
    25861 gccagttctc cagccaggct tgcgcgacga caggatcgta gtaacccgcc agcaacagga
    25921 ttgagtcaat atcacgcgtc aggcgtggca actggtcacg gtagcgatcg cccaacggct
    25981 ggcagaaaac gcttttcagt tcagcggcat ggcgggaaag atggatatcg gcaaagcgtt
    26041 tgaaggaatc gctcatttta ctctgcgctt tggcatctaa aaacggctgc caggctttgc
    26101 ttaccagcca ttcggtcaat gccagcttcg ccattgccgt ttcggtagag tagaccgccg
    26161 tcacggcaga aaccgctgaa gcgatggtcg cttcgcattg agtcaataaa tcacgtaagt
    26221 gagtgctcgc tttacgcggc acaatgccac cgaacagcat cagcgtatga cggaccaggc
    26281 caatggctgc cagcacctgc tctttcgccg catcattgcc gcgtacccat agttcttcat
    26341 gatactgcca ttgcgctaac gccagctcga gcgccgcttc cagcccctgt tccacatcag
    26401 cttttgccgc aacatgtaaa atggtggtcg gtttgatttc acgcgccgga ttgccctgcg
    26461 ccagatgata gccacgcgcc gctttgctca gactgccctg gcgtaatccg gtttgcgata
    26521 ccagttggtt cgccagtttc agcaccgcgc gcgtgtcgcc gctaagcagt tccagttcca
    26581 gctcgcagat aggttcagca aattcacccg ctttcacttc cccctggtcg agggcgattt
    26641 caatttggct accatcaacc tctaccagcc acttttcgcg ataaaaatcg gtgctaaaca
    26701 gcggctgcac gcgggaagcg agatcggtag gcaattcgcc gttcggccag acttccgtcg
    26761 gtaactgcgc caggtcgagc gtcggttcgc tcaacgccac gttatattcc gggcgctggt
    26821 gtaagccgcc tgtcactctt cctgcaactt tcatggtcat ctcatagcga ccgttttcgc
    26881 cacgaatacg caagcccatg tcgtgcccac gcagccagtt atccggcgtt tcgtagtaaa
    26941 tattcagcaa ctgcacgggg tcatggtgct cgccgcccag cgtattgaga tggtcacgca
    27001 acgcctcaac ggcactgtga ttaacaataa actttaattc gatttcctga gccatagcct
    27061 ttacttatcg gttatgtcac ataagtgacg atgaacggcg aacttaatgc gatctttttt
    27121 gtcagtagat agtattttgc gccaaattgc catgcaacgg gcaatttgac gggcgtaaaa
    27181 gtttgaagca gtggcaagac ctgacacaga ggattccgat tgatgtcgaa tgctttgcgt
    27241 agcgacactg atgccactac tatcgttcca ctttccatga caataacgac agcctgatgc
    27301 caaaattacg cctgatcgga ttaactttac tcgcacttag cgccactgcc gtctcacacg
    27361 ctgaagaaac gcgctatgtt tctgatgaac tgaatacctg ggtccgtagc ggtccgggag
    27421 atcattatcg cctcgtgggc acggttaacg ccggcgagga agtgacctta ttacaaactg
    27481 acgccaacac caattatgcc caggtgaaag acagctctgg ccgtaccgcc tggatcccgt
    27541 tgaaacaact tagcacagag ccaagcctgc gctcccgtgt gccagatctg gaaaatcagg
    27601 tcaaaaccct gaccgataaa ctcaccaata tcgataacac ctggaaccaa cgcaccgcag
    27661 aaatgcagca aaaagtggcg cagagcgaca gcgtgatcaa cgggttaaaa gaagaaaatc
    27721 agaaactgaa aaacgagctg attgtcgcgc agaaaaaggt cgatgccgcc agcgtacagc
    27781 tggatgacaa acagcgcacc atcatcatgc agtggtttat gtatggcggt ggcgtgctgg
    27841 ggcttggctt gctgcttggt ctggtactgc cgcacctgat cccaagccgc aaacgcaaag
    27901 atcgctggat gaactaaatc gccttcttcg ttgcactgtc ttattattag gaattgtgta
    27961 tttgacgaag ggatgatggc gtgaagattt atctggtcgg tggtgctgtt cgggatgcat
    28021 tgttagggct accggtcaaa gacagagatt gggtggtggt cggcagtacg ccacaggaga
    28081 tgctcgacgc gggctaccag caggtaggcc gcgattttcc tgtgtttctg catccgcaaa
    28141 cgcatgaaga gtatgcactg gcacgtaccg aacggaaatc tggttccggt tacaccggtt
    28201 ttacctgcta tgccgcaccg gatgtcacgc tggaagatga tcttaagcgt cgcgatctga
    28261 ccattaatgc gctggcccag gacgataacg gcgagattat cgacccgtac aacggtctgg
    28321 gcgatctgca aaatcgtctg ttgcgccatg tttcccccgc ttttggcgaa gatccgttac
    28381 gcgtgttgcg cgtagcgcgt tttgctgcgc gttatgccca cctcagtttc cgtattgccg
    28441 atgaaactct ggcgctgatg cgcgagatga cccatgcggg tgaactggaa cacctgacgc
    28501 ctgaacgggt atggaaagag acggaaaacg ccctcaccac ccgcaatccc caggtgttct
    28561 tccaggtact gcgcgattgc ggcgcactgc gcgttttatt cccggaaatt gacgccctgt
    28621 ttggcgttcc ggcccctgcc aggtggcatc cggaaatcga cacgggtatt cataccttaa
    28681 tgacgctctc aatggcggcg atgctgagtc cgcaggtcga tgtccgtttc gcaacgttat
    28741 gccacgatct cggtaaagga ctgacgccac cagaactttg gccgcgtcat catggtcatg
    28801 gcccggcggg tgtgaagtta gtggaacaac tatgccagcg tctgcgcgtg ccaaatgaaa
    28861 ttcgcgattt agccagactg gtggctgagt ttcacgatct catccacacc ttcccaatgc
    28921 tgaacccaaa aaccatcgtc aaactatttg attccatcga cgcctggcgt aaaccgcagc
    28981 gtgtcgagca actggcgctg accagcgagg ctgatgtgcg cggcagaacc ggttttgaat
    29041 cagccgacta cccgcaaggc cgctggttgc gcgaagcctg ggaagtggca cagtcagtgc
    29101 cgacaaaagc cgtcgttgaa gcgggattta aaggtgtgga gattcgcgag gagctgaccc
    29161 gacggcggat tgcggcggtg gctggctgga aggaacagcg ttgcccaaaa cctgagtgag
    29221 tgttggatat tttgccggat gcgacgtaag cgtcttatcc ggctacatgt caacgacagt
    29281 tgtaggcctg ataagacgcg cgagcgtcgc atcaggcgtt gattgccgga tgcggcgtga
    29341 acgccttatc cggcctacgc aatctgataa gacgcgtcag catcatatcc gacactgttc
    29401 ttcacatcaa aagaacacaa cgtacaccgc agccgccaca ataaagcgat aaatggcgaa
    29461 cgggataaac gaaatgcgct taatcaattg caggaaggtt ttaatcgcta tcagcgccac
    29521 cacaaaagcg gtgataaacc caacggcaaa catcgggata tcgccggtag tcaggaagcc
    29581 ccagcttttg tagagatcga gcgccgttgc gcccatcatc atcggcaccg ccagcaggaa
    29641 cgagaactcg gaagcagcgt aacggctcac ccccatcagc atcccacctg aaatggtcgc
    29701 cccggaacgg gagaaacccg gccacagcgc cagacactgg aaacagccaa tcataaacgc
    29761 ctgacgatag gtcatatcat caagacccgg cgcacgcggc tctttcggct tcaggcattc
    29821 ggcggcaatc agcaacagac cgccaacgac cagtgcatac atcacattta tcgggttaaa
    29881 caatgattta atcgtgtcgt ggaacaacag ccccagtacc accgccggaa tcatccccag
    29941 caaaatgtgg atcagcgtta aacggccttt gctttctcct tcgtgctgca acgggcgacc
    30001 aaagtgaata ccaataaggc caaacagacg tcgccagaac atgacaacca ctgccagaat
    30061 tgatcctaac tggatcacga cttcaaaggt tttcgccgtg tcgccctcaa accccaacaa
    30121 gtgaccgaca ataatcatat ggcccgtgct ggataccggc agaaattctg tcaatccttc
    30181 gaccacaccc aatattgccg ctatcagcag cgagtgcata tcgctcatca ataaacccct
    30241 aaattataaa aatgtaacgc ttgtccgaac tactgcagta tgaccaggtt ataaccgttt
    30301 ggtttaacag ctgtaaaatt aattattttc tttcagatta ttgccacgct caatgattac
    30361 gccaacattc gccgcccgcg ccactgcgcc tggcttgctg agtttgatac gcacccacgg
    30421 cgagttgaag cgtgctaaca gcaactccgc cacctcttca gccacacgtt ccaccagcgc
    30481 aaaacgcgcc ccctcgacgt ggctgactac cgtttctgca atgtcagcgt aactgaggca
    30541 atccgccaca tcatcacttt tcgccgcttt acggttatcc cacgccattt cgatatcgaa
    30601 cactaacttc tgttcgatgg tctgttccca gtcgtaaaca ccaatagtgg tgattaccga
    30661 aagttgctct ataaatacaa tatccatcac gtcctgcctg ctttttggct aaccggatac
    30721 cacttccggc gaaatgtgcg tattatccac agattcatcg ttgaacacga attttcaaaa
    30781 cggaacagct tatgagtgca atcgcgcctg gaatgatcct catcgcgtac ctctgcggct
    30841 ccatttccag tgccattctg gtttgccgct tgtgtgggct gcccgatccg cgaaccagcg
    30901 gctccggcaa tccaggcgca accaatgtgt tacgtatcgg tggcaaggga gcagccgtag
    30961 cagtactgat tttcgacgtt ctgaaaggaa tgttgcccgt ctggggcgcg tatgaattag
    31021 gtgtcagccc cttctggcta ggcttaattg ccatcgccgc ctgtcttgga cacatctggc
    31081 ccgttttctt cggatttaaa ggaggaaaag gcgttgctac cgcttttggt gccatcgcac
    31141 ccattggctg ggatctcacc ggagtaatgg cgggaacctg gttactgacc gtgctattga
    31201 gcggatattc gtcgctgggg gcgattgtca gtgcactgat tgctccgttt tatgtctggt
    31261 ggtttaagcc acaattcacc ttcccggttt cgatgctctc ttgcctgatc ctgctgcgcc
    31321 atcatgacaa catccagcgt ctgtggcgtc gtcaggagac aaaaatctgg acgaaattca
    31381 aaagaaagcg cgaaaaggat cccgagtgat ttctggtgga gctacataac ctgatagcct
    31441 tcgtcgggct tgcccagccg ttgctggcac catgccgcca gaaattccac gcagacacgt
    31501 aatttcatgc tgcgatagag cggctcccgg taaacagccc agatattggc gctttgtgca
    31561 tactccggca atactcgcac cagtttgcca ctctccagaa acggcaacac atcccactcg
    31621 gaacgcaaca taatcccttt gccctccagc gcccattgca acactatctc gccgctattg
    31681 gaggaaagat gcccgcttac cttcaccgat tttttctcct gaccgttccc caactcccat
    31741 attccgtggg tcatatcgcg ctctttggtc accaggcagt catgacgact taattcctgt
    31801 aaggattgtg gctgcggata tttttgcaga tactcaggtg ctgcgcataa tattctttta
    31861 ttttttgtca aaagatgcgc aatataataa tcaggaattg cgtcattaat acgaatatcc
    31921 agatcaatat tatcctgcac taaatcaatt tgccgatcga acaattcaaa atgcacctgt
    31981 aactcaggat aattgcgcat cagttcggta atggctggag caatatggct gcgcccaaaa
    32041 ccaaaactgc aaccaatgcg gatcatcccc tccgggcgcg ttttgatttg cgtgacgtca
    32101 tcgaccagtc gctgatattg ggtgaggatt tccagcgcat gttcgtaaca acgttggcca
    32161 ctttcagtca gcgccacgcc gcgagcggag cggttcagca gagttgttgc cagcgtattt
    32221 tcaaggattt ggatccgctt ggtgacaaat gcaggcgttt gcccaagcgt cgccgctgca
    32281 gcactaaaac tcccgctatg gacgatttcc accagtacct gtaagtcttt ggctaaaggc
    32341 cagctattta gcattgttaa atatccgcag ttttgataaa cgcagtgtat ttcgcaaaac
    32401 attaccattc cctgctctgg cgcaatattc gttcttattt tcgcgttgat tcacgaactg
    32461 ttaattacat attcacggtg gcaaaaaata taaaaccaca tttttagtgg tagtttgtgg
    32521 cggtgaattt ttccagacaa atacataaac tggagttgcc atgatgagcg aaagtaataa
    32581 gcaacaggca gtgaataagt tgacggagat tgtcgctaac tttaccgcca tgatttctac
    32641 ccgaatgcct gatgacgtgg tggataaact aaaacagcta aaggatgccg aaacatcgtc
    32701 gatgggaaaa atcatctacc acacgatgtt cgataacatg caaaaagcga tcgacctgaa
    32761 tcgtcctgcc tgtcaggaca ccggcgaaat catgtttttt gttaaggtcg gttcccgttt
    32821 cccactgctt ggcgagctgc aaagcatcct caaacaagcc gtggaagagg caaccgtcaa
    32881 agcgccactg cgtcacaatg cggtagaaat ttttgacgaa gtgaacaccg gcaaaaatac
    32941 cggcagcggc gtaccgtggg tcacctggga tatcgtcccc gacggtgacg atgcggaaat
    33001 cgaagtttac atggcaggcg gcggctgcac actacctggt cgttcgaaag tgttaatgcc
    33061 gtcggaagga tacgaaggcg tggtgaaatt cgtcttcgaa aatatctcca ccctcgccgt
    33121 aaacgcctgt ccaccggtac tggtaggcgt gggcatcgcc acctcggtgg aaaccgccgc
    33181 tgtgctctca cgtaaagcca ttttacgccc aattggcagc cgccacccca atccgaaagc
    33241 ggcagagctg gagctacgcc tggaagaagg actcaaccgt ctggggattg gtccacaagg
    33301 gctgactggc aacagttcag tgatgggcgt acatatcgaa tctgccgccc gccatccgtc
    33361 aaccatcggc gttgctgttt ctacaggctg ctgggcgcat cgtcgcggca cactgctggt
    33421 tcatgccgat ctcacctttg aaaatctgtc tcacacccgg agcgcgttat gaaaaagatc
    33481 ctgacaaccc cgatcaaagc tgaagatctg caagatattc gcgtcggcga tgtgatctac
    33541 ctgaccggta cgctggtgac ctgccgtgac gtttgtcacc gtcgcttgat cgaactcaaa
    33601 cgtccgatcc cttacgatct caacggcaaa gcgattttcc acgctggccc catcgtgcgc
    33661 aaaaacggcg acaaatggga gatggtatcc gtcggcccga caaccagtat gcgtatggaa
    33721 agttttgaac gtgaatttat cgaacagacc ggcgtgaaac tggtggtcgg caaaggcgga
    33781 atggggccgc tgaccgaaga aggctgccag aaattcaagg cgctgcatgt gattttcccg
    33841 gcaggctgcg cagtactggc ggcaacccag gtggaagaga ttgaagaagt gcactggaca
    33901 gaactcggga tgccggagtc gctgtgggtc tgccgggtca aagagttcgg tccgctgatt
    33961 gtttctattg atacccacgg caacaacctg atagccgaaa acaaaaagct gttcgccgaa
    34021 cgccgcgatc ccatcgtgga agagatttgc gagcacgtcc actacatcaa ataaccctcc
    34081 cggagaggct caccccctct cctttttcgc aggcataaca cgatgaaacc ttccactgaa
    34141 tggtggcgat accttgcgcc gctggcggtc atcgccatta ttgctctaat tccggttccc
    34201 gcagggctgg agagtcatac ctggctctac tttgccgttt ttactggcgt gatcgttgga
    34261 ctgatcctcg aacccgtgcc gggtgccgtg gtggcgatgg tgggtatctc cattatcgcc
    34321 attctctcgc cgtggctgct gtttagcccg gagcagctcg cccagccagg ttttaagttc
    34381 accgccaaat ccctctcctg ggcggtttct ggtttttcta attcggttat ctggctgatt
    34441 ttcgccgcct ttatgtttgg cacaggctat gaaaaaaccg ggcttggacg ccgcattgcg
    34501 ctgattctgg tgaaaaagat gggacatcgc acgctgtttc tcggctatgc ggtgatgttc
    34561 tccgagctga tcctggcacc tgtaacaccg tccaactcgg cgcgcggtgc ggggattatc
    34621 tatcccatca tccgtaacct gccaccgctc tatcaatcac aaccaaacga cagcagttcg
    34681 cgcagcattg gctcgtacat catgtggatg gggattgttg ccgactgcgt gaccagcgcc
    34741 attttcctga cggcgatggc acctaacttg ctgttaattg gcctgatgaa aagcgcatct
    34801 cacgccacac tgagttgggg cgactggttc ctcgggatgt tgccgctcag tattttactg
    34861 gttctgctgg ttccctggct ggcttacgtg ctgtacccgc cggtactgaa gtctggtgat
    34921 caggtgccgc gctgggcaga gacggaactg caggcaatgg gcccgctctg ttcgcgtgaa
    34981 aaacggatgc tggggctgat ggtaggcgcg ctggtgctgt ggattttcgg cggtgattat
    35041 atcgatgccg cgatggtcgg ttacagcgtg gtggcgctga tgctgcttct gcgcattatc
    35101 agttgggacg acattgtcag taataaagcc gcgtggaacg ttttcttctg gctggcctcg
    35161 cttatcaccc tcgctaccgg actcaacaac accggtttta ttagctggtt tggcaaactg
    35221 ttagcaggca gcttaagcgg ttattcgcca acgatagtga tggtggcgtt gattgtggtg
    35281 ttttatctac tgcgctactt tttcgccagc gccacggcgt atacctccgc tctcgcgccg
    35341 atgatgattg ccgccgcgct ggcgatgccg gaaatcccgc tgccggtgtt ctgcctgatg
    35401 gttggcgcgg caattggtct ggggagcatt cttacgccat acgccaccgg acccagtccg
    35461 atttactacg gtagtggtta tctgccaacg gtggattact ggcgactggg ggcaattttt
    35521 gggctgatat tcctcgtgtt gctggtgatt accggcttac tgtggatgcc cgtggtgttg
    35581 ctttaagtga ggacggtgcc ggaagtgact ctccggcacc taagagtttt acgcggccgg
    35641 taactctgcc agcggccagc gcggacgcac gctaacgcca agatccgccg tcgcgcctgc
    35701 tttaaaccgc accattccgg cataggcgat catcgcgccg ttatcagtac aaaattccgg
    35761 acgcgcgtag aacacttcgc cacggcgttt tttcatcatt tccgccagct tcgcccgtaa
    35821 cgtgcggtta gcactcacgc cacctgccat taccagtcgc ttaaagcccg tctgatccag
    35881 cgctcgctta cacttaatca tcaacgtatc gaccaccgca tcttcaaagg cgcgggcgat
    35941 atcagcacgc gtctggtcgt cggtgccgtt atcacgaatg gtatttgccg cgaaggtttt
    36001 cagaccagaa aagctgaaat ccagccctgg acggtcggtc atcgggcgcg ggaagacaaa
    36061 gcgcccggct gtaccctgag ccgccatttt cgacagtaat ggcccgccag gataatccag
    36121 ccccagcagc ttcgcggttt tatcaaacgc ttcaccagcc gcatcatcga tagactcgcc
    36181 gagcagctcg tactgaccaa tgccagtcac gctgattaac tgcgtatgac cgccggaaac
    36241 cagcagcgcc acaaacggaa attccggtgg gttatcttcc agcatcggcg ctaacagatg
    36301 cccttccata tggtgtacag ggatcgccgg aacgttccag gcaaacgcca gagaacgccc
    36361 cacggtcgcg ccaaccagca gtgcgccgac taatccaggg cctgcggtat aggccacagc
    36421 atcaatgtct tttgccgtta aaccagactc ctttagcgcc gcctggatca acggtacggt
    36481 tttacgcaca tgatcgcggg aggccagctc aggcactacg ccgccgtagt cagcgtgcaa
    36541 tttcacctga ctatacaatt ggttggctaa caaacctttt tcatcgtcgt aaatggcgat
    36601 gccggtttca tcgcaggaag tttcaatacc cagtacacgc atgacttgtt ttacctcgct
    36661 ttattaccgc gcagtgtagg accaatgcgg gttgatgtaa aactttgttc gcccctggag
    36721 aaagcctcgt gtatactcct cacccttata aaagtccctt tcaaaaaagg ccgcggtgct
    36781 ttacaaagca gcagcaattg cagtaaaatt ccgcaccatt ttgaaataag ctggcgttga
    36841 tgccagcggc aaaccgaatt aatcaaaggt gagaggcaca tgccggtaat taaagtacgt
    36901 gaaaacgagc cgttcgacgt agctctgcgt cgcttcaagc gttcctgcga aaaagcaggt
    36961 gttctggcgg aagttcgtcg tcgtgagttc tatgaaaaac cgactaccga acgtaagcgc
    37021 gctaaagctt ctgcagtgaa acgtcacgcg aagaaactgg ctcgcgaaaa cgcacgccgc
    37081 actcgtctgt actaattccc cgagagcgtt gctctccgat cagaccgagt tgtagttgta
    37141 aggccgtgct tccgaaagga atgcgcggct tattttcgtt tatgaattgc taaaaatcgg
    37201 ggcctatggc tggacgaatc ccacgcgtat tcattaatga tctgctggca cgcactgaca
    37261 tcgtcgatct gatcgatgcc cgtgtgaagc tgaaaaagca gggcaagaat ttccacgcgt
    37321 gttgtccatt ccacaacgag aaaaccccgt cattcaccgt taacggtgag aaacagtttt
    37381 accactgctt tggatgtggc gcgcacggca acgcgatcga cttcctgatg aactacgaca
    37441 agcttgagtt cgtcgaaacg gtcgaagagc tggcggcaat gcacaatctt gaagtgccat
    37501 ttgaagcagg tagcggcccc agccagatcg agcgccatca acgacaaacg ctttatcagt
    37561 tgatggacgg tctgaatacg ttttaccaac aatctttaca gcaacctgtt gccacgtctg
    37621 cgcgccagta tctggaaaaa cgcggattaa gccacgaggt tatcgcccgc tttgcgattg
    37681 gttttgcccc ccctggctgg gacaacgtcc tgaagcggtt tggcggcaat ccagaaaatc
    37741 gccagtcatt gattgatgcg ggcatgttgg tcactaacga tcaggggcgc agttacgacc
    37801 gtttccgcga gcgggtgatg ttccccattc gcgataaacg cggtcgggtg attggttttg
    37861 gcgggcgtgt gctgggcaac gatacaccca aatacctgaa ctcgccggaa acggacattt
    37921 tccataaagg ccgccagctt tacggtcttt atgaagcgca gcaggataac gctgaaccca
    37981 atcgtctgct tgtggtcgaa ggctatatgg acgtggtggc gctggcgcaa tacggcatta
    38041 attacgccgt tgcgtcgtta ggtacgtcaa ccaccgccga tcacatacaa ctgttgttcc
    38101 gcgcgaccaa caatgtcatt tgctgttatg acggcgaccg tgcaggccgc gatgccgcct
    38161 ggcgagcgct ggaaacggcg ctgccttaca tgacagacgg tcgtcagcta cgctttatgt
    38221 ttttgcctga tggcgaagac cctgacacgc tggtacgaaa agaaggtaaa caagcgtttg
    38281 aagcgcggat ggagcaggcg atgccactct ccgcatttct gtttaacagt ctgatgccgc
    38341 aagttgatct gagtacccct gacgggcgcg cacgtttgag tacgctggca ctgccactga
    38401 tatcgcaagt gccgggcgaa acgctgcgaa tatatcttcg tcaggaatta ggcaacaaat
    38461 taggcatact tgatgacaac cagcttgaac gattaatgcc aaaagcggca gagagcggcg
    38521 tttctcgccc tgttccacag ctaaaacgca cgaccatgcg tatacttata gggttgctgg
    38581 tgcaaaatcc agaattagcg acgttggtcc cgccgcttga gaatctggat gaaaataagc
    38641 tccctggact tggcttattc agagaactgg tcaacacttg tctctctcag ccaggtctga
    38701 ccaccgggca acttttagag cactatcgtg gtacaaataa tgctgccacc cttgaaaaac
    38761 tgtcgatgtg ggacgatata gcagataaga atattgctga gcaaaccttc accgactcac
    38821 tcaaccatat gtttgattcg ctgcttgaac tgcgccagga agagttaatc gctcgtgagc
    38881 gcacgcatgg tttaagcaac gaagaacgcc tggagctctg gacattaaac caggagctgg
    38941 cgaaaaagtg atttaacggc ttaagtgccg aagagcgatc gggaagcccc cgacagccgc
    39001 actgagaggc agcggcaaat atataagtac gccctcgtaa ttatcgttgg cggtaaacaa
    39061 ccgttggatt tcagcgttaa cggctgaagg acatcgggtc aatcgcccaa caccaacctc
    39121 atgaaataag tgtggatacc gtcttatgga gcaaaacccg cagtcacagc tgaaacttct
    39181 tgtcacccgt ggtaaggagc aaggctatct gacctatgcc gaggtcaatg accatctgcc
    39241 ggaagatatc gtcgattcag atcagatcga agacatcatc caaatgatca acgacatggg
    39301 cattcaggtg atggaagaag caccggatgc cgatgatctg atgctggctg aaaacaccgc
    39361 ggacgaagat gctgccgaag ccgccgcgca ggtgctttcc agcgtggaat ctgaaatcgg
    39421 gcgcacgact gacccggtac gcatgtacat gcgtgaaatg ggcaccgttg aactgttgac
    39481 ccgcgaaggc gaaattgaca tcgctaagcg tattgaagac gggatcaacc aggttcaatg
    39541 ctccgttgct gaatatccgg aagcgatcac ctatctgctg gaacagtacg atcgtgttga
    39601 agcagaagaa gcgcgtctgt ccgatctgat caccggcttt gttgacccga acgcagaaga
    39661 agatctggca cctaccgcca ctcacgtcgg ttctgagctt tcccaggaag atctggacga
    39721 tgacgaagat gaagacgaag aagatggcga tgacgacagc gccgatgatg acaacagcat
    39781 cgacccggaa ctggctcgcg aaaaatttgc ggaactgcgc gctcagtacg ttgtaacgcg
    39841 tgacaccatc aaagcgaaag gtcgcagtca cgctgccgct caggaagaga tcctgaaact
    39901 gtctgaagta ttcaaacagt tccgcctggt gccgaagcag tttgactacc tggtcaacag
    39961 catgcgcgtc atgatggacc gcgttcgtac gcaagaacgt ctgatcatga agctctgcgt
    40021 tgagcagtgc aaaatgccga agaaaaactt cattaccctg tttaccggca acgaaaccag
    40081 cgatacctgg ttcaacgcgg caattgcaat gaacaagccg tggtcggaaa aactgcacga
    40141 tgtctctgaa gaagtgcatc gcgccctgca gaaactgcag cagattgaag aagaaaccgg
    40201 cctgaccatc gagcaggtta aagatatcaa ccgtcgtatg tccatcggtg aagcgaaagc
    40261 ccgccgtgcg aagaaagaga tggttgaagc gaacttacgt ctggttattt ctatcgccaa
    40321 gaaatacacc aaccgtggct tgcagttcct tgacctgatt caggaaggca acatcggtct
    40381 gatgaaagcg gttgataaat tcgaataccg ccgtggttac aagttctcca cctacgcaac
    40441 ctggtggatc cgtcaggcga tcacccgctc tatcgcggat caggcgcgca ccatccgtat
    40501 tccggtgcat atgattgaga ctatcaacaa actcaaccgt atttctcgcc agatgctgca
    40561 agagatgggc cgtgaaccaa cgccggaaga actggctgaa cgtatgctga tgccggaaga
    40621 caagatccgc aaagtgctga agatcgccaa agagccaatc tccatggaaa cgccgatcgg
    40681 tgatgatgaa gattcgcatc tgggggattt catcgaggat accaccctcg agctgccgct
    40741 ggattctgcg accaccgaaa gcctgcgtgc ggcaacgcac gacgtgctgg ctggcctgac
    40801 cgcgcgtgaa gcgaaagttc tgcgtatgcg tttcggtatc gatatgaaca ccgaccacac
    40861 gctggaagaa gtgggtaaac agttcgacgt tacccgcgaa cgtatccgtc agatcgaagc
    40921 gaaggcgctg cgcaaactgc gtcacccgag ccgttctgaa gtgctgcgta gcttcctgga
    40981 cgattaatcg gtaggccgga tcaggtgtta cgccgcaccc ggcactagtc cctccgcaca
    41041 aacgccacct tttcggtggc gcgaaaattg atgtaatgca atgaaaaata aaggattatt
    41101 tttctcgttg tccacatttt gccatagcga tacatcttag cccgtaatga ttcgcgggct
    41161 aagcatatac aaaatgttaa gcctgatgtt ggaaaaagtt tgtttttcct ttgtgtagga
    41221 aagaacaaag ggtggcggaa agttcagaaa gccagcgatt atggcgtaat tattctgaca
    41281 gaagaacaat ttatcaattt tattgatact ggtgaaattc ctcttgagca agactgcgtg
    41341 gttgagaatg atatcagtaa atcgcttgag aaggatgtta acacttacaa gagtatgata
    41401 gatgagttct cgctttctat ccgtactgta agggagtttc gccgtagtaa tgcgttgatt
    41461 gcggtatttg ttgatggtta cgcagctggt tggcgctttg ctgtaaaaga gtgttacaag
    41521 gattctttgg atataaaact taccccagta aaaacagagt tttttaatta tgaaatttgg
    41581 acgcaagggg attcctttca gtttaatcgc ggtgatgttt tttatagtga tcgcctagcg
    41641 tatgattctt attgcgattt tttaaagctt gatgatgctg tcgttcttca ggtgaaatat
    41701 gagtgctttt ctggttacga ttctgtaagt acttttgatg gtgtgatgga tggtgagttt
    41761 tctttttctc gtatggaacc cagacctgtc cataaattac ctgtgctgat tgaaactcaa
    41821 ctgtatgacc ccggaaagat tattgttgat gtttttgttc ctgatgctgc gaaaaaacaa
    41881 ttaaaactta aagacacctt agagctgaag caggatgaat ttgtctgttt acttcagtca
    41941 gggtgttgtt gggttaaacg gagggaagca ggagataaga aaaaacctga atttattaac
    42001 ctattcaaaa aataaaaata tagcccgcat atcgcgggca aatgtctatg taattcaacg
    42061 gagaaagggt gccatatcga ccgaacccgg caaccggata gcgggggctt tcacccccgc
    42121 tgcggtttct tactgtttac actgcaagaa cgccgcaaat tgtgaccaca tgatgttaag
    42181 cctgaattca cacggtgacg tgtgtatcat ccagacaatc atcaaaatag ttatgcataa
    42241 aatgctcact atgatcgttt tttgcggcat agcgcttgac tccttttaca gggaggcgct
    42301 aaccttccac ttgcgaggta gatgggttag ggcctcgggt taaacaaatg tttgactcgg
    42361 ggcctttcca catccggcct tcaggtaatc cttccagccg tcagccgaaa ggcacccgcg
    42421 cgtaatttac cttttacgtc cgttacgtgc aacggatgac gtggctgttt ttccttcccc
    42481 ttcgccatcc tttttcggag cagttgtgca aaagtgtgca aaagtgattt ttgttgccac
    42541 tccccgccag gccgcgccag ttctggcttt cctgattttt tcgttttcgt c
//
LOCUS       DAZGQL010000033        42116 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.033, whole genome shotgun sequence.
ACCESSION   DAZGQL010000033 DAZGQL010000000
VERSION     DAZGQL010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42116
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.033"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     misc_feature    77..201
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            83..127
                     /gene="pheM"
                     /locus_tag="ACHKIR_002058"
     CDS             83..127
                     /gene="pheM"
                     /locus_tag="ACHKIR_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HFO9989802.1"
                     /translation="MNAAIFRFFFYFST"
     gene            411..1394
                     /gene="pheS"
                     /locus_tag="ACHKIR_002059"
     CDS             411..1394
                     /gene="pheS"
                     /locus_tag="ACHKIR_002059"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFO9989803.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            1409..3796
                     /gene="pheT"
                     /locus_tag="ACHKIR_002060"
     CDS             1409..3796
                     /gene="pheT"
                     /locus_tag="ACHKIR_002060"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFO9989804.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVESVAGSFHG
                     VVVGEVIECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGAAIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            3801..4100
                     /gene="ihfA"
                     /locus_tag="ACHKIR_002061"
     CDS             3801..4100
                     /gene="ihfA"
                     /locus_tag="ACHKIR_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HFO9989805.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            4201..5181
                     /gene="btuC"
                     /locus_tag="ACHKIR_002062"
     CDS             4201..5181
                     /gene="btuC"
                     /locus_tag="ACHKIR_002062"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HFO9989806.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGELWISPGDWF
                     SPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            5244..5795
                     /gene="btuE"
                     /locus_tag="ACHKIR_002063"
     CDS             5244..5795
                     /gene="btuE"
                     /locus_tag="ACHKIR_002063"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HFO9989807.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGGTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            5795..6544
                     /gene="btuD"
                     /locus_tag="ACHKIR_002064"
     CDS             5795..6544
                     /gene="btuD"
                     /locus_tag="ACHKIR_002064"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HFO9989808.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQHQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLFLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            6623..7087
                     /gene="nlpC"
                     /locus_tag="ACHKIR_002065"
     CDS             6623..7087
                     /gene="nlpC"
                     /locus_tag="ACHKIR_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFO9989809.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKQQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            7334..8047
                     /gene="ydiV"
                     /locus_tag="ACHKIR_002066"
     CDS             7334..8047
                     /gene="ydiV"
                     /locus_tag="ACHKIR_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HFO9989810.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQDLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLDRDNYAG
                     ELLKYPFIELLINENYPHLDEGKDNRDLLSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTPEIMAQITPFDFHAL
                     QGCLWPAVPVNQITTLVQR"
     gene            8110..9546
                     /gene="selO"
                     /locus_tag="ACHKIR_002067"
     CDS             8110..9546
                     /gene="selO"
                     /locus_tag="ACHKIR_002067"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO"
                     /protein_id="HFO9989811.1"
                     /translation="MTLSFVTRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNGAGVWGGETLLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQRLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVALSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FSDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRRRLQQDEVSDIERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHEAL
                     RNPFSDRDDDYASRPPDWGKRLEVSCSS"
     gene            complement(9550..9741)
                     /gene="hemP"
                     /locus_tag="ACHKIR_002068"
     CDS             complement(9550..9741)
                     /gene="hemP"
                     /locus_tag="ACHKIR_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HFO9989812.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(9873..10919)
                     /gene="aroH"
                     /locus_tag="ACHKIR_002069"
     CDS             complement(9873..10919)
                     /gene="aroH"
                     /locus_tag="ACHKIR_002069"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HFO9989813.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            complement(11076..11909)
                     /gene="ppsR"
                     /locus_tag="ACHKIR_002070"
     CDS             complement(11076..11909)
                     /gene="ppsR"
                     /locus_tag="ACHKIR_002070"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HFO9989814.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            12242..14620
                     /gene="ppsA"
                     /locus_tag="ACHKIR_002071"
     CDS             12242..14620
                     /gene="ppsA"
                     /locus_tag="ACHKIR_002071"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HFO9989815.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(14677..16323)
                     /gene="fadK"
                     /locus_tag="ACHKIR_002072"
     CDS             complement(14677..16323)
                     /gene="fadK"
                     /locus_tag="ACHKIR_002072"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HFO9989816.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGATYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKTGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSALSLSQIIADNTPLTTAITVHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWQDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPAACLALLEQQ
                     RCTCMLGATPFVYDLLNLLEKQPADLSALRFFLCGGTTIPKKVARECQQRGIKLLSVY
                     GSTESSPHAVVNLDDPLSRFIHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEDVVAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASGKIQKFLLRKDIMRRLTQDVCEEIE"
     gene            complement(16380..16673)
                     /gene="ydiT"
                     /locus_tag="ACHKIR_002073"
     CDS             complement(16380..16673)
                     /gene="ydiT"
                     /locus_tag="ACHKIR_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFO9989817.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIEFRYG"
     gene            complement(16670..17959)
                     /gene="ydiS"
                     /locus_tag="ACHKIR_002074"
     CDS             complement(16670..17959)
                     /gene="ydiS"
                     /locus_tag="ACHKIR_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFO9989818.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLDAIIPGFAASAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVSASDPHHYAVGVKEVIGLTPEQINDRFNVTGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSVSLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNDGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSASSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(18014..18952)
                     /gene="ydiR"
                     /locus_tag="ACHKIR_002075"
     CDS             complement(18014..18952)
                     /gene="ydiR"
                     /locus_tag="ACHKIR_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HFO9989819.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQTV
                     MPYGPKCIYVLAQNDALQRTENYAESIAALLKDKHPSMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSN
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(18972..19736)
                     /gene="ydiQ"
                     /locus_tag="ACHKIR_002076"
     CDS             complement(18972..19736)
                     /gene="ydiQ"
                     /locus_tag="ACHKIR_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HFO9989820.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYMVQDAQLEHALPLDTAKALA
                     TAVEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIGWSQSAPLAEL
                     TGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
     gene            20052..20963
                     /gene="ydiP"
                     /locus_tag="ACHKIR_002077"
     CDS             20052..20963
                     /gene="ydiP"
                     /locus_tag="ACHKIR_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO9989821.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLAIL
                     YYENFKNAYRSEQGYIKKDVLIKDILFYLNNNYREKITLEQLSKKFRASVSYICHEFT
                     KEYRISPINYVIQRRMTEAKWSLTNTELSQAEISWRVGYENVDHFAKLFLRHVGCSPS
                     DYRRQFKNCFAEQEILSEFPQPVSLAG"
     gene            complement(21006..22157)
                     /gene="ydiO"
                     /locus_tag="ACHKIR_002078"
     CDS             complement(21006..22157)
                     /gene="ydiO"
                     /locus_tag="ACHKIR_002078"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HFO9989822.1"
                     /translation="MDFSLTEEQELLLASIRELITTNFPEEYFRTCDQNGTYPREFMR
                     ALADNGISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESTLETGDPAYALALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITGAK
                     EYPYMLVLARDPQPKDPKKAFTLWWVDSSKPGIKINPLHKIGWHMLSTCEVYLDNVEV
                     EESDMVGEEGMGFLNVMYNFEMERLINAARSTGFAECAFEDAARYANQRIAFGKPIGH
                     NQMIQEKLALMAIKIDNMRNMVLKVAWQADQHQSLRTSAALAKLYCARTAMEVIDDAI
                     QIMGGLGYTDEARVSRFWRDVRCERIGGGTDEIMIYVAGRQILKDYQNK"
     gene            complement(22171..23766)
                     /gene="ydiF"
                     /locus_tag="ACHKIR_002079"
     CDS             complement(22171..23766)
                     /gene="ydiF"
                     /locus_tag="ACHKIR_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HFO9989823.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SDLAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFIRATTCDSEGYATFEDEVMYLDALVIAQAVHNN
                     GGIVMMQVQKMVKKATLHPKSVRIPGYLVDIVVVDPDQTQLYGGAPVNRFISGDFTLD
                     DSTKLSLPLNQRKLVARRALFEMRKGAVGNVGVGIADGIGLVAREEGCADDFILTVET
                     GPIGGITSQGIAFGANVNTRAILDMTSQFDFYHGGGLDVCYLSFAEVDQHGNVGVHKF
                     NGKIMGTGGFIDISATSKKIVFCGTLTAGSLKTEITDGKLNIVQEGRVKKFIRELPEI
                     TFSGKIALERGLDVRYITERAVFTLKEDGLHLIEIAPGVDLQKDILDKMDFTPVISPE
                     LKLMDERLFIDAAMGFVLPEAAH"
     gene            complement(23912..24670)
                     /gene="aroD"
                     /locus_tag="ACHKIR_002080"
     CDS             complement(23912..24670)
                     /gene="aroD"
                     /locus_tag="ACHKIR_002080"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFO9989824.1"
                     /translation="MKTVTVKDLVIGTGAPKIIVSLMAKDIASVKSEALAYREADFDI
                     LEWRVDHYADLSNVESVIAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDIIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(24701..25567)
                     /gene="ydiB"
                     /locus_tag="ACHKIR_002081"
     CDS             complement(24701..25567)
                     /gene="ydiB"
                     /locus_tag="ACHKIR_002081"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HFO9989825.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(25579..26844)
                     /gene="ydiN"
                     /locus_tag="ACHKIR_002082"
     CDS             complement(25579..26844)
                     /gene="ydiN"
                     /locus_tag="ACHKIR_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9989826.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMTSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYCLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFATFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFIRY
                     YRVFIIPENDVRFGEHKFSTRLNTIKHRG"
     gene            complement(27071..28285)
                     /gene="ydiM"
                     /locus_tag="ACHKIR_002083"
     CDS             complement(27071..28285)
                     /gene="ydiM"
                     /locus_tag="ACHKIR_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9989827.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILHTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALLLYRCTFPPHPGRHLPVIKKTTSSTEHRCSIIDLASYSLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALLTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSAGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFILALFIGLRSRKETR
                     HHSLKENVAPGG"
     gene            complement(28385..28741)
                     /gene="ydiL"
                     /locus_tag="ACHKIR_002084"
     CDS             complement(28385..28741)
                     /gene="ydiL"
                     /locus_tag="ACHKIR_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HFO9989828.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTDDSESWRQWENGKCAIP
                     DRVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTVFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(28877..28984)
                     /gene="rprA"
                     /locus_tag="ACHKIR_002085"
     ncRNA           complement(28877..28984)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="ACHKIR_002085"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(29170..30282)
                     /gene="ydiK"
                     /locus_tag="ACHKIR_002086"
     CDS             complement(29170..30282)
                     /gene="ydiK"
                     /locus_tag="ACHKIR_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HFO9989829.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKSNK"
     gene            30669..33725
                     /gene="ydiJ"
                     /locus_tag="ACHKIR_002087"
     CDS             30669..33725
                     /gene="ydiJ"
                     /locus_tag="ACHKIR_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HFO9989830.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDETLIDERVNALCARLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSSMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALDSQPVATVSGESWY
                     FFGHCTEVTALPGAPAQWVAIFARFGAKLENVSVGCCGMAGTYGHEAKNHKNSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            33722..34132
                     /gene="menI"
                     /locus_tag="ACHKIR_002088"
     CDS             33722..34132
                     /gene="menI"
                     /locus_tag="ACHKIR_002088"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HFO9989831.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGLLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGVC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            34232..34420
                     /gene="ydiH"
                     /locus_tag="ACHKIR_002089"
     CDS             34232..34420
                     /gene="ydiH"
                     /locus_tag="ACHKIR_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HFO9989832.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            34969..35337
                     /gene="sufA"
                     /locus_tag="ACHKIR_002090"
     CDS             34969..35337
                     /gene="sufA"
                     /locus_tag="ACHKIR_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HFO9989833.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAVHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            35346..36833
                     /gene="sufB"
                     /locus_tag="ACHKIR_002091"
     CDS             35346..36833
                     /gene="sufB"
                     /locus_tag="ACHKIR_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFO9989834.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            36843..37589
                     /gene="sufC"
                     /locus_tag="ACHKIR_002092"
     CDS             36843..37589
                     /gene="sufC"
                     /locus_tag="ACHKIR_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFO9989835.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLL
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            37564..38835
                     /gene="sufD"
                     /locus_tag="ACHKIR_002093"
     CDS             37564..38835
                     /gene="sufD"
                     /locus_tag="ACHKIR_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFO9989836.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGAKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLDGLTNSQFVSIAGEISPQQRDALALTLDAVRLVFVDGRYVPALSDATE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPLSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            38832..40052
                     /gene="sufS"
                     /locus_tag="ACHKIR_002094"
     CDS             38832..40052
                     /gene="sufS"
                     /locus_tag="ACHKIR_002094"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HFO9989837.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNNIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            40065..40481
                     /gene="sufE"
                     /locus_tag="ACHKIR_002095"
     CDS             40065..40481
                     /gene="sufE"
                     /locus_tag="ACHKIR_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HFO9989838.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRDEDKSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            40630..41634
                     /gene="ldtE"
                     /locus_tag="ACHKIR_002096"
     CDS             40630..41634
                     /gene="ldtE"
                     /locus_tag="ACHKIR_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HFO9989839.1"
                     /translation="MKCASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYNYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKGVDQKLVDKALYRRAGYPVAVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(41698..41934)
                     /gene="lpp"
                     /locus_tag="ACHKIR_002097"
     CDS             complement(41698..41934)
                     /gene="lpp"
                     /locus_tag="ACHKIR_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HFO9989840.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
BASE COUNT        10541 a        10305 c        10915 g        10355 t
ORIGIN      
        1 aacgccatca aaacattgac ttttatcgcc gtagcctttt caataaaggt cttttgaaga
       61 gtaccaaaag gtaacgcaag caatgaatgc tgctattttc cgcttctttt tttactttag
      121 cacctgactc caggaggcta gcgcgtgaga agagaaacgg aaaacagcgc ctgaaagcct
      181 cccagtggag gctttttttg tatgcgcgtt ttgaaaaata aaaagcctgt ctcatcaggc
      241 gtaattgtcg cagccagtct ggacacggcc agcgcggaga aaccggagcg tacttaagta
      301 cgtgaggatt tcgagcactg cccgggacca aaatggcaag taaaatagcc tgatgggatg
      361 ggctctaagt ccaacgaacc agtgtcacca ctgacacaat gaggaaaaac atgtcacatc
      421 tcgcagaact ggttgccagt gcgaaggcgg ccattagcca ggcgtcagat gttgccgcgt
      481 tagataatgt gcgcgtcgaa tatttgggta aaaaagggca cttaaccctt cagatgacga
      541 ccctgcgtga gctgccgcca gaagagcgtc cggcagctgg tgcggttatc aacgaagcga
      601 aagagcaggt tcagcaggcg ctgaatgcgc gtaaagcgga actggaaagc gctgcactga
      661 atgcgcgtct ggcggcggaa acgattgatg tctccctgcc gggtcgtcgc attgaaaacg
      721 gcgggctgca tccggtgacc cgtaccatcg accgtatcga aagtttcttc ggtgagcttg
      781 gctttaccgt ggcaaccggg ccggaaatcg aagacgatta tcataacttc gatgctctga
      841 acattcctgg tcaccacccg gcgcgcgctg accacgacac tttctggttt gacgctaccc
      901 gcctgctgcg tacccagacc tctggcgtac agatccgcac catgaaagct cagcagccac
      961 cgattcgtat catcgcgcct ggccgcgttt atcgtaacga ctacgaccag actcacacgc
     1021 cgatgttcca tcagatggaa ggtctgattg ttgataccaa catcagcttt accaacctga
     1081 aaggcacgct gcacgacttc ctgcgtaact tctttgagga agatttgcag attcgcttcc
     1141 gtccttccta cttcccgttt accgaacctt ctgcagaagt ggacgtcatg ggtaaaaacg
     1201 gtaaatggct ggaagtactg ggctgcggga tggtgcatcc gaacgtgctg cgtaacgttg
     1261 gcatcgaccc ggaagtttac tctggtttcg ccttcgggat ggggatggag cgtctgacca
     1321 tgttgcgtta cggcgtcacc gacctgcgtt cattcttcga aaacgatctg cgtttcctca
     1381 aacagtttaa ataaggcagg aatagattat gaaattcagt gaactgtggt tacgcgaatg
     1441 ggtgaacccg gcgattgata gcgatgcgct ggcaaatcaa atcactatgg cgggcctgga
     1501 agttgacggt gtagaatcgg ttgccggtag cttccacggc gtggtcgttg gtgaagtgat
     1561 tgagtgtgcg cagcatccga acgctgacaa actgcgtgtg acaaaagtga atgtcggcgg
     1621 cgatcgcctg ctggacatcg tctgcggtgc gccaaactgc cgtcagggcc tgcgtgtggc
     1681 ggtagcgacc attggtgctg ttctgccggg cgatttcaaa attaaagcgg cgaaactgcg
     1741 tggcgaaccg tctgaaggga tgctgtgctc cttttctgag ctgggaattt ctgacgatca
     1801 cagcggcatt atcgagctgc ctgcagatgc gccgattggc accgacatcc gcgaatacct
     1861 gaaactcgat gacaacacca tcgaaatcag cgtaacgcca aaccgtgccg attgcttagg
     1921 cattatcggc gtggcgcgtg atgttgccgt gctgaaccag ctgccgctgg ttgaaccgga
     1981 aatcgttccg gttggtgcgg ccatcgacga cacgctgccg attacagtcg aagcgccgga
     2041 agcctgcccg cgctatcttg gccgtgtggt aaaaggcatt aacgttaaag cgccaactcc
     2101 gctgtggatg aaagaaaaac tgcgtcgttg cgggatccgt tctatcgatg cagttgttga
     2161 cgtcaccaac tatgtgctgc tcgaactggg ccagccgatg cacgctttcg ataaagatcg
     2221 cattgaaggc ggcattgtgg tgcggatggc gaaagagggc gaaactctgg tgctgcttga
     2281 tggcactgaa gcgaagctga atgctgacac cctggtcatc gccgaccaca acaaggcgct
     2341 ggcgatgggc ggcatctttg gtggcgaaca ctctggcgtg aatgacgaaa cgcaaaacgt
     2401 cctgctggaa tgcgctttct tcagcccgct gtctatcacc ggtcgtgctc gtcgtcatgg
     2461 cctgcatact gatgcgtctc accgttatga gcgtggcgtt gatccggcac tgcagcacaa
     2521 agcgatggaa cgtgcgaccc gtctgctgat cgacatctgc ggtggtgagg ctggtccggt
     2581 aattgatatc accaacgaag caacgctgcc gaagcgtgcc accattactt tacgtcgcag
     2641 caaactggat cgcctgatcg gccatcatat tgctgatgag caggtaactg acattctgcg
     2701 tcgtctcggc tgcgaagtga ccgaaggcaa agacgaatgg caggccgttg cgccgagctg
     2761 gcgtttcgac atggagattg aagaagatct ggtcgaagaa gtcgcgcgtg tttacggcta
     2821 caacaacatc ccggatgagc cggtacaggc aagcctgatt atgggtactc accgcgaagc
     2881 cgatctgtcg ctcaagcgcg tgaaaacgct gctcaacgac aaaggctatc aggaagtgat
     2941 cacctacagc ttcgttgatc cgaaagtgca gcagatgatc catccaggcg ttgaagcctt
     3001 actgctgcca agccctattt ccgttgaaat gtcagcaatg cgtctttccc tgtggaccgg
     3061 cctgctggca accgtggtgt acaaccagaa ccgtcagcag aaccgtgtgc gcattttcga
     3121 aagtggtctg cgtttcgtac cagatactca ggcaccgttg ggcattcgtc aggatctgat
     3181 gttagccggt gtgatttgcg gtaaccgtta cgaagagcac tggaacctgg caaaagagac
     3241 cgttgatttc tatgatttga aaggcgatct tgaatccgtt ctcgacctga ccggtaaact
     3301 gaatgaggtt gagttccgtg cagaagcgaa tccggcactg catccggggc aatccgcagc
     3361 gatttatctg aaaggtgaac gtattggttt tgttggggtt gttcatcctg aactggaacg
     3421 taaactggat cttaacggtc gcactctggt gttcgaactg gagtggaaca agctcgcaga
     3481 ccgcgtggtg cctcaggcgc gcgagatttc tcgcttcccg gcgaaccgtc gtgacatcgc
     3541 ggtggtggtc gcagaaaacg ttcccgcagc ggatatttta tccgaatgta agaaagttgg
     3601 cgtaaatcag gtagttggcg taaacttatt tgacgtgtac cgcggtaagg gtgttgcgga
     3661 ggggtataag agcctcgcca taagcctgat cctgcaagat accagccgta cactcgaaga
     3721 agaggagatt gccgctaccg tcgccaaatg tgtagaggca ttaaaagagc gattccaggc
     3781 atcattgagg gattgaacct atggcgctta caaaagctga aatgtcagaa tatctgtttg
     3841 ataagcttgg gcttagcaag cgggatgcca aagaactggt tgaactgttt ttcgaagaga
     3901 tccgtcgcgc tctggaaaac ggcgaacagg tgaaactctc tggttttggt aacttcgatc
     3961 tgcgtgataa gaatcaacgc ccgggacgta acccgaaaac gggcgaggat attcccatta
     4021 cagcacggcg cgtggtgacc ttcagacccg ggcagaagtt aaaaagccgg gtcgaaaacg
     4081 cttcgcccaa agacgagtaa tctgatctaa ctaaaaaggc cgctctgcgg ccttttttct
     4141 tttcactgtc gaagagtcac cgtaaaatca acgccatgac acttcagcag aacggatacc
     4201 atgctgacac ttgcccgcca acaacagcga caaaatattc gctggttatt atgcctgtca
     4261 gttttgatgc tgctggcgct tctcttaagc ctttgcgccg gtgaactatg gatctcgcca
     4321 ggtgactggt tttctcctcg tggcgaactg ttcgtctggc aaattcgcct gccacgtacg
     4381 ctggctgtat tgctggttgg tgcggcgctg gctatatccg gcgctgtaat gcaggcgttg
     4441 tttgaaaatc ctctggcaga acctggacta cttggcgtct ctaacggcgc aggcgtgggg
     4501 cttatcgccg cggtattgct tgggcaaggg caactcccca actgggcgct agggctgtgt
     4561 gcgattgctg gcgcgcttat catcacttta atactcttac gtttcgctcg tcgtcatctt
     4621 tcgaccagtc ggttattgct ggctggcgtt gcattaggga ttatctgtag cgcactaatg
     4681 acgtgggcta tctacttttc cacctctgtt gatttacgtc agctgatgta ctggatgatg
     4741 ggcggttttg gcggcgtaga ctggcggcaa agctggctga tgctggcatt gatccccgtg
     4801 ttgttgtgga tctgttgtca gtccaggccg atgaatatgt tagcacttgg cgagatctcg
     4861 gcgcggcaac tgggtttacc cctgtggttc tggcgcaatg tgttggtcgc agcgaccggc
     4921 tggatggttg gcgtcagtgt ggcgctggcg ggtgctatcg gctttattgg tctggtgatc
     4981 ccacatattc tccggctgtg tggtttaacc gatcatcgcg tattacttcc cggctgcgcg
     5041 ctggcagggg cgagcgcatt gctgctggcc gatattgtag cgcgcctggc attagctgcc
     5101 gcagagctgc ctattggcgt ggtcaccgca acgttgggtg cgccggtgtt tatctggtta
     5161 ttgttaaaag caggacgtta gccgcaaaaa gacggtctat gattaaaagc taagattttt
     5221 actgaccaca cccaggagaa acgatgcaag attccattct gacgaccgta gtgaaagata
     5281 tcgacggtga agtgaccacg ctggagaagt acgccggtaa tgtgctgttg attgtcaatg
     5341 tcgcctcaaa gtgtggctta acgccgcaat atgagcagtt ggagaatatt cagaaagcct
     5401 gggccgatcg tggttttgtg gtgctgggat tcccgtgcaa ccagtttctg gaacaagaac
     5461 cgggcagcga tgaagagatt aaaacttact gtaccaccac atgggggggg acgttcccga
     5521 tgttcagtaa gattgaagtt aatggcgaag gacgccatcc gctgtatcaa aaattgattg
     5581 ccgcagcgcc gaccgcagtc gcgccggaag agagcggatt ctatgctcgt atggtcagca
     5641 aaggtcgtgc accgctgtac cctgatgata ttttatggaa ttttgagaaa tttttagttg
     5701 gcagggacgg attggtcatc cagcgttttt ccccggatat gacgccggaa gatcccattg
     5761 tgatggaaag cattaaactg gcgttggcaa aataatgtct attgtgatgc agttacagga
     5821 tgttgcggaa tctacccgcc tggggccgct ttctggcgag gttcgggctg gggagatcct
     5881 gcacctggtg gggccgaatg gcgcgggtaa gagtacctta ctggcgcgaa tggccggaat
     5941 gaccagcggt aagggaagca ttcagttcgc ggggcaacca ctggaagcat ggtccgcaac
     6001 aaaactcgcg ctacatcgcg cctatctttc acaacaccag acgccaccgt ttgcaatgcc
     6061 ggtctggcac tacctgacac tgcatcagca cgataaaacg cgtaccgaac tactgaatga
     6121 tgtcgcaggg gcgctggctc ttgatgacaa actcggacgg agcaccaatc aactttccgg
     6181 cggtgaatgg caacgcgtac gtcttgctgc ggtggtgttg caaatcacac cacaagccaa
     6241 tcccgcaggc caattgctgt ttcttgatga gccgatgaac agtcttgatg ttgcgcaaca
     6301 aagtgcgtta gacaaaattc tgagcgcact gtgtcagcaa ggactggcga ttgtgatgag
     6361 cagtcacgat ctcaaccaca ccttgcgtca tgcgcatcgg gcgtggttgc taaaaggtgg
     6421 aaaaatgctg gccagtggac gcagggaaga ggtgctcacg ccgccaaatc tggcgcaggc
     6481 ctatgggatg aattttcgcc gtctggatat cgaaggtcac agaatgctga tttcgaccat
     6541 ctgatttcat taatatcttg aaaatcgggt aattacgacg ctaaattaat gcaagaataa
     6601 aaaaacagag gaaccgtacg gaatgcgttt ctgccttatt ttgatcacag cactgtttct
     6661 ggccgggtgt agccaccata aagcaccgcc gccaaatgcc agactttctg attcgattac
     6721 cgttattgcc ggtttgaacg accagctaca aagctggcat ggcacgccgt atcgttatgg
     6781 tggcatgacg cggcgcggtg tggactgttc ggggtttgtg gttgtgacga tgcgcgatcg
     6841 tttcgatttg cagctgcccc gtgaaaccaa acaacaagcc tctatcggca cgcaaattga
     6901 taaagacgag ttgctgcctg gtgacctggt ctttttcaaa acgggctccg gacaaaatgg
     6961 tttgcatgta ggtatttatg ataccaacaa ccaatttatc cacgcctcta ccagcaaggg
     7021 agtgatgcgt tcctcacttg ataatgtcta ttggcagaaa aatttctggc aggcgagacg
     7081 aatctagtat gtcgaaaaat ggacggcgat gatacatcgc cgtccattta ctcctgaata
     7141 cagtagtatt ttgcagacaa aaattccgta aatggattat tcgtttataa tcaggataat
     7201 ctaaaaaaag gacccctgaa aaagtggtta acatggaaca aatgaaagtt actccttctg
     7261 taattgttat gtcttatggc ctcatccctt ttagccggat actgaaaaac atccttcgag
     7321 agggacggtt accatgaaga tttttttgga gaatctttat cattctgatt gctatttcct
     7381 gccgataaga gataaccagc aagaccttgt cggcgttgag ttaattaccc atttctccag
     7441 tgaagatggt acggttcgta tcccgacgag tcgggttata gcgcaactta ccgaggagca
     7501 acattggcaa ttattttcag agcagttgga attactaaaa tcatgccaac acttttttat
     7561 tcaacataaa ctctttgcct ggcttaattt aacgccacaa gtggctacat tattattaga
     7621 cagggataat tacgcaggcg aactattaaa atatccgttt attgaactgt tgattaatga
     7681 gaattacccg catctcgatg aaggaaaaga taatcgcgat cttttgtcct tatcgcaaat
     7741 gtatcccctt gtgttaggta atttgggcgc aggcaatagc acaatgaaag cggtttttga
     7801 tggtcttttc acccgcgtca tgctggataa aagttttatt cagcaacaaa tcacgcatcg
     7861 ctccttcgaa ccgtttattc gtgcgatcca ggctcaaatt tccccatgtt gcaattgcat
     7921 catcgcgggg ggcattgata cgccagaaat aatggcacag attaccccct ttgatttcca
     7981 tgctctccag ggatgcctgt ggcctgccgt tcctgtaaat cagataacga cgttggttca
     8041 gcgataaccc tcctgtttgc tggtgtttaa gacgagagta accgtctaca ctatcaaata
     8101 ggaggatcta tgaccctgtc ttttgttacc cgctggcgcg atgaattgcc agaaacctat
     8161 acagcacttt cccctacgcc tttaaataat gcccggctga tttggcataa taccgaactc
     8221 gctaacacgc tgagtattcc atcgtcgctg tttaaaaatg gcgcaggtgt ctggggcggc
     8281 gaaaccctac tgcctggtat gtcaccactg gcgcaggttt acagtggtca tcagttcggc
     8341 gtctgggcgg gccaactggg tgatgggcgc ggcattttac tcggcgaaca acggcttgct
     8401 gatggcacta caatggactg gcatctgaaa ggtgctggcc tgacgcctta ttcgcgaatg
     8461 ggtgatggac gggcggtttt acgttcgacg atacgagaaa gtctcgccag tgaggcgatg
     8521 cattatctgg gcattccgac gacccgcgcg ttaagtatcg tcaccagcga ttcgccagtg
     8581 tatcgggaaa cggtggagcc aggcgcgatg ctgatgcgtg tggcactaag tcatctgcgc
     8641 tttggtcatt tcgaacattt ttactatcgc cgcgagccgg aaaaggttcg tcagttggct
     8701 gactttgcca ttcgtcatta ctggtctcat cttgaagatg atgaggacaa ataccgtctc
     8761 tggtttagcg atgttgtcgc acgtaccgca tcgttaattg cccaatggca gacggtcggc
     8821 tttgctcatg gggtgatgaa taccgacaac atgtcgctgc tggggctgac gcttgattac
     8881 gggccgtttg gttttcttga tgattacgaa cccggtttta tttgtaatca ctcggatcac
     8941 caagggcgtt acagctttga taatcaacct gccgtcgcgt tgtggaattt acagcgtctg
     9001 gcgcagacat tgtcgccatt tgtcgccgtt gatgccctga atgaggccct ggacagctat
     9061 cagcaggttt tgttgacgca ttatggacaa cggatgcggc agaaactggg cttcatgacg
     9121 gagcaaaaag aggataacgc gctactaaat gaattattca gtctgatggc gcgagagcgc
     9181 agcgattata cccgcacatt tcgcatgctg agtctgaccg agcagcacag cgcggcgtca
     9241 ccgctacgtg atgagtttat tgatcgtgcg gcatttgatg actggtttgc ccgttatcgg
     9301 aggcgtttgc aacaagacga ggttagcgat attgagcgtc agcaactgat gcaaagcgtt
     9361 aacccagctc tggtgttgcg caactggttg gcgcaacggg cgattgaagc ggcagaaaag
     9421 ggtgatatga cggaattgca ccgcctgcat gaggcgttgc gaaatccttt cagcgacaga
     9481 gatgatgact acgccagtcg tccacctgac tggggtaaac ggctggaagt cagctgctcg
     9541 agttaaaggt tacttggtca acagcagctt gccagcctga gttttacgga gcagatattc
     9601 ttgcccgtca tgatcgataa tcagtttgcc atccggacct aacagtgtct ggctggtaat
     9661 tcgccggatg ggctgcgggg aagcggtctt gtgattggca tccgtttcag gcgtgagttt
     9721 tctgctatcc gtataacgca taaaaattaa taataattta tataacaatg attcttatta
     9781 tcaatattat ggcgggccag agcaagcagt aatttgttga gaagacaaca agttgcgcag
     9841 aaatgcccat cccgtcagga atgggcacgc attcagaagc gagtatctac cgcagaggcg
     9901 agtttttcga ccaggcgttc ggtatcttcc cagcccagac acgggtcggt aatggattga
     9961 ccgtaagtga gcggctgacc gccgacgatt ttttgcgttc cttcgcgcag gaaactttcc
    10021 gccataattc cagcaatcgc cgtagatcca ttgcggattt gctgacaaat atcctcacaa
    10081 acttctaact ggcgtcggtg ctgcttctgg cagttaccgt ggctgaaatc caccaccaga
    10141 tgttcaggta aatcaaactc gtgcagcgta tcgcaggctg cggcgatatc atcggcatga
    10201 taattcggtt ttttgccgcc acgcataata atgtggccat acgggttgcc gctggtctga
    10261 tagatggtca tctgaccatt tttgtctggc gagaggaaca tatggctggc gcgggctgcg
    10321 cggatagcat ccacggcaat ccgcgtgttg ccatcggtac catttttaaa acctaccgga
    10381 caggagagtg ccgaagccat ttcacggtgg atctgacttt cggtagtacg tgcgccaatc
    10441 gcgccccaac tgattaaatc agcaataaac tgaccggtca ccatatcgag gaactcagtc
    10501 gcggtaggga cgcccagctc atttacctgt aaaagtaatt tgcgcgccag ctccagaccg
    10561 tgatttaccc gatagctgcc gtttaaatct ggatcggaga ttagcccttt ccagccgaca
    10621 acagttcgtg gtttttcaaa ataggtgcgc attacgattt ccagccgtga ctggtactgg
    10681 ttgcgcagcg actgcagacg ggtggcgtac tccattgcag cggtgagatc gtggatcgag
    10741 caggggccaa taatgaccaa cagtcgctta tcttcaccat tcagtatttt ttcaattctg
    10801 cggcgggagt cggtgacatg ggtggcgacg ccaggcgtta cgggataccg tagcgcgagt
    10861 tcggcgggcg ttaccaggct ctcaatacgc gcagtacgga gttcgtcagt tctgttcatt
    10921 acaagtctca gaaatttgtt gcttctacgg caggactacc gggaagtgat gcgcatcacg
    10981 ataaaccaat cgcagctgat ttcaagtgcg aggtgtgtca cacctcgccg ggtggttagc
    11041 atgataacaa aaaaacaagc taatgcacta gttctctagt acattcgacg gctaaggccc
    11101 atgatatcga ggatcttggt ggcaatctct tctaccgaat aattggtact gttaatccac
    11161 gggatctgat ttttacggta caacgcttcc acttccgcga cttccatcct gcactgacga
    11221 agcgaggcat agcgactgtt ctcccgacgt tcctcgcgaa tcgccgccag acgttccggg
    11281 tcgatagtca ggccgaacaa tttatgctga agcggtttga gcgacgcggg tagcacaaga
    11341 ttatccatat cgtcggcaat aaaggggtag tttgccgcgc ggataccaaa ctgcattgcc
    11401 agatacagac tggtgggggt tttaccacag cgagaaacgc cgagcaggat cacctgagcc
    11461 tggtccagat tgcgtaacga aatgccatca tcgtgggcga gggtgtaatc aatcgcagca
    11521 atgcgcgcat catatttatt gagattatta gggttaaggc catgggtacg atgagcaatc
    11581 ggcgttggat ccagtttcat ctcttgttgt agcggggcaa ccagcgcctg aacgatatcc
    11641 tggcaaaagc cctcactttg caagatgatg gcgcgaatct ccggcaacac gatggagtag
    11701 aagaccagcg ggcgcacacc tgtctggtga taaattgcgt caatctgatc cttcactgcc
    11761 cgtgcacggc tctcattttc gacaaacggc agcgtaatgc tgctgatagt gacgggaaat
    11821 tgtgacatta ctgcgtgtcc taatacctcc gcagttattg ccgtaccatc agaaatataa
    11881 aaaacgtggc gatcaacagc attatccatt ttgttcttcc cgtgatgcag acataattct
    11941 atgaaagcat aaattaaaaa cgcacagaag cgtagaacgt tatgtctggt ttataaaatg
    12001 aaccttcaat tttatttttt atgaaaacag catttcattt ttacggtttc gtttataccg
    12061 atggtttatg tggaaattgt cgaagagagc agatttgcgc aacgctggga tcagtcttaa
    12121 aaagtaaaaa aatatatttg cttgaacgat tcaccgtttt tttcatccgg ttaaatatgc
    12181 aaagataaat gcgcagaaat gtgtttctca aaccgttcat ttatcacaaa aggattgttc
    12241 gatgtccaac aatggctcgt caccgctggt gctttggtat aaccaactcg gcatgaatga
    12301 tgtagacagg gttgggggca aaaatgcctc cctgggtgaa atgattacta acctttccgg
    12361 aatgggtgtt tccgttccga atggtttcgc cacaaccgcc gacgcgttta accagtttct
    12421 ggaccaaagc ggcgtaaacc agcgcattta tgaactgctg gataaaacgg atattgacga
    12481 tgttacccag cttgcgaaag cgggcgcgca aatccgccag tggattatcg acactccctt
    12541 ccagcctgag ctggaaaacg ccatccgcga agcctatgca cagctttccg ccgatgacga
    12601 aaacgcctcg tttgcggtgc gttcctccgc tactgcagaa gatatgccgg acgcttcttt
    12661 tgccggtcag caggaaactt tcctcaacgt tcagggtttt gacgccgttc tcgtggctgt
    12721 gaaacatgta tttgcttctc tgtttaacga tcgcgccatc tcttatcgtg tgcaccaggg
    12781 ttacgatcac cgtggcgtgg cgctgtccgc cggtgttcag cggatggtgc gctccgacct
    12841 cgcatcttct ggcgtgatgt tctccattga taccgaatcc ggctttgacc aggtggtgtt
    12901 tatcacttcc gcatggggcc ttggtgaaat ggtcgtgcag ggtgcggtta acccggatga
    12961 gttttacgtg cacaaaccga cactggcggc gaatcgcccg gctatcgtgc gccgcaccat
    13021 ggggtcgaaa aaaatccgca tggtttacgc gccgacccag gagcacggca agcaggttaa
    13081 aatcgaggac gtaccgcaag aacagcgtga catcttctcg ctgaccaacg aagaagtgca
    13141 ggaactggca aaacaggccg tacaaattga gaaacactac ggtcgcccaa tggatattga
    13201 gtgggcgaaa gatggccaca ccggtaaact gttcattgtg caggcgcgtc cggaaaccgt
    13261 gcgctcacgc ggtcaggtca tggagcgtta tacgctgcat tcacagggta agattatcgc
    13321 cgaaggccgt gctatcggtc atcgcatcgg tgcgggtccg gtgaaagtca tccatgacat
    13381 cagcgaaatg aaccgcatcg aacctggaga cgtgctggtc actgacatga ccgacccgga
    13441 ctgggaaccg atcatgaaga aagcatctgc catcgtcacc aaccgtggcg gtcgtacctg
    13501 tcacgcggcg atcatcgctc gtgaactcgg cattccggcg gtagtgggct gtggtgatgc
    13561 aacagaacgg atgaaagacg gtgagaacgt cactgtttct tgtgccgaag gtgataccgg
    13621 ttacgtctat gcggagttgc tggaatttag cgtgaaaagc tccagcgtag aaacgatgcc
    13681 tgatctgccg ttgaaggtga tgatgaacgt cggtaacccg gaccgtgctt tcgacttcgc
    13741 ctgcctgccg aacgaaggcg tcggccttgc gcgtctggaa tttatcatca accgtatgat
    13801 tggcgtccac ccacgcgcac tgcttgagtt tgacgatcag gaaccgcagt tgcaaaacga
    13861 aatccgcgag atgatgaaag gttttgattc tccgcgtgaa ttttacgttg ggcgtctgac
    13921 tgaagggatc gcgacgctgg gtgccgcgtt ttatccgaag cgcgtcattg tccgtctctc
    13981 tgattttaaa tcgaacgaat atgccaacct ggtcggtggt gagcgttacg agccagatga
    14041 agagaacccg atgctcggct tccgtggcgc gggccgctat gtttccgaca gcttccgcga
    14101 ctgcttcgcg ctggagtgtg aagcagtgaa acgtgtgcgc aacgacatgg ggctgaccaa
    14161 cgttgagatc atgatcccgt tcgtgcgtac cgtagatcag gcgaaagcgg tggttgagga
    14221 actggcgcgt caggggctga aacgtggtga gaacgggctg aaaatcatca tgatgtgtga
    14281 aattccgtcc aacgccttgc tggcagagca gttcctcgaa tatttcgacg gcttctcaat
    14341 tggctcaaac gacatgacac agctggcgct cggtctggac cgtgactccg gagtggtgtc
    14401 tgaactgttc gatgagcgca acgatgcggt gaaagcactg ctgtcgatgg cgattcgtgc
    14461 cgcgaagaaa cagggcaaat atgtcgggat ttgcggtcag ggtccgtccg accacgaaga
    14521 ctttgccgca tggttgatgg aagaggggat cgatagcctg tctctgaacc cggataccgt
    14581 ggtgcaaacc tggttaagcc tggctgaact gaagaaataa aataaatccc cggcggcgtt
    14641 ttgtcgccgg gtttatgtga tccccgaaga tgaaacttat tcaatctctt cacagacatc
    14701 ctgagttaat cgccgcataa tatctttcct taacaaaaac ttttgtattt tacctgaggc
    14761 tgtgcgcggt agtttttcga ttaccacgat atgttcagga tatttatatt tcgcgacccg
    14821 tttacggcta aaaaatgcca ctacatcttc cagcgataat gaatgatgcg gtgctttcag
    14881 cacgacataa gcgcatgaac gttcacctaa gcgttcatcg ggcattgcaa ccacacaagc
    14941 atcgtgaatt ttaggatgct gcaataaaat atcttctact tcacggctgc taatattttc
    15001 gccgccgcgg acaataatat ctttcttgcg cccggttatt tttatatagc cagcctcatc
    15061 catgcggcag agatcgccgc tgtaatacca gccttcttca tccagcgcac gggcggttaa
    15121 ttcaggttca tcaaaatacc ccataaacac attggggcca cgcgaggctt cttcaccttc
    15181 gcaacctggc ggtaaagtct tgcgtgcgtc atcgaccact ttaatctcta cacctgcggc
    15241 agcgtaacca tcggtgtgta taaagcgcga caaaggatca tcgagattca ccaccgcatg
    15301 cggcgaactt tctgtggaac cataaacact taataattta atgccgcgct gctggcattc
    15361 acgcgccact tttttgggga ttgtggtacc gccgcaaaga aagaaacgca gcgctgaaag
    15421 gtctgcgggc tgtttctcca gtaaattcaa aagatcatag acaaacggcg ttgcgccgag
    15481 catacaggtg caacgctgct gctcaagcag cgcgagacac gcagcaggag tgaaaatatc
    15541 taacaacacg ctgcgagcac caatcaaaaa aggtgctgtt acgccatgca gaaagcccgt
    15601 tgcgtgacca agtggcgcag gcatcataaa gacatcctgc caggtcagat ttagccgcgc
    15661 gcaataagcc cgctcactgg cgagaatatt gttatgcgtt agcatcacgc cctttggcag
    15721 accctcggtt ccggaggtaa acagcaccgc ggctaattca tcgccgtgga ccgttatcgc
    15781 cgtggtcaga ggagtattgt cggcgataat ctgacttaat gagagagcag aggtggcggg
    15841 agccagtttg tccacgccga caatttgttg tagttgtgga agctgatttt gcagcggcag
    15901 gattaaatct accggacgcg tttgtttaaa caacgtcggt gcaaagaaca tttttgcctg
    15961 acacttattg agcacccaca ccagttctgc ttctcgccag gaaggcaaca gcggcacgga
    16021 aaccgcaccg gttttcaggc aggcaagata gataacggta aattcacacc agcctggcag
    16081 ttgaaatgcg atgcgatcgc ctgattcaat acccttcgct aacatccagt ttgccagaca
    16141 gctcgcggcg tgatcgagcg cgctataagt gtacgttgca ccatgattgt cgaccacggc
    16201 aattttgtct ggcatcgcac gagcggtctg ctgccagtaa tcggccagtg aagcatcgcc
    16261 ccataaccct tgctgacgat acgccgcacg acgttgttcg ttgaacgtta atgtcacttt
    16321 cattttggac tctcgaaaca gaacatcagg cccgagatgc gggcctgtgg gatgcatgat
    16381 tagccgtagc gaaattcaat accgaaggtg cctgcgggat attgccactg ttcgagaata
    16441 gtgttgccgc acagcacccg acaggtgccg cactccagac aaccggcgga atcgaaatga
    16501 atgtttcctg cgtcatcctg cttataaagt ccggcagggc aggctttcat taatttacgg
    16561 aattcattga tatcgggatt tgccgccaga atgatatgcg ggtggccctc atcaacatgg
    16621 aatttattta cgcctaattt gatgtcgacg ttaaccgtag cgttctggct catagcgcgg
    16681 ttgctccctt aatgccatct ttcagcaagt tgatcagtcc aattttcttc gcgtgtccca
    16741 tgatcatttt gcgtaccggt tggttaggtt tgccgtcgat ggtgaacatc tcgttcatga
    16801 tgtcggcgac cattcgtggg tattggctaa acaggcgcgg gttttccatc agcgccggga
    16861 tcttgcgaaa atgctgcata tcacgcatga cgcagctttg ttccagctca cgtttgtatt
    16921 gcgcaagact gctggcggag aaatccgcgc gttctttggc ggcgatcact gttgtggcgg
    16981 cagcctgagc cgatgcaatg gctaaatcca tgccgcggac cgtaaaaccc aaattcaggc
    17041 agaagcctgc ggcgtcacca acgatcatca cgccatcgtt aaccagctgc ggcaccattg
    17101 ccagaccgcc ctccggcacc atatgcgcgg agtattcaag cagtttgccg ccgctaatca
    17161 gcgggcgaat ggcggggtgt tgtttaaaat cttccagcat ttgcggcaca cttttttgcg
    17221 catgggcgat atcacccaat ccacaaacca gccccaacga tacggaatct ttgttggtat
    17281 agaggaatcc gccgcccatc aggccgtcag aaggggaacc ggcaaacagc caggcggcac
    17341 cttcctcgcc cgtaacatta aagcgatcgt tgatctgttc tggtgtcagg cctataacct
    17401 ctttaacacc aacagcgtaa tgatgcggat cggaagcgga aaccattccc agcgagcggc
    17461 caagcatcga gttaacgcca tcagccagaa tcaccacatt cgcttcgaga atatcatccc
    17521 cggcctggac gccagtgacc ttgtttcctt cacgaaccag tgcatcgacg cgtacgcccg
    17581 ggataaactg cgcgcccgcc tgctcggctt gttccatcaa ccacgggtcc agacgattac
    17641 gcaatacggt ataagacgcg tgttgcggaa catctggttg ctcgcggtga aaatcgaggg
    17701 taacggcgct ttcttcggtt aagaaggaga ttttctcgcg tgtgaccttg cgttctaccg
    17761 gcgctgatgc tgcaaagcct ggaataattg cgtcaagtgt gtgggcataa agacgcccgc
    17821 cggtcatgtt tttacatccg gcactgtcgc cgcgttctat caccaggaca tccagcccgg
    17881 ctcgcgccat gaccagtgcg gcaacgctac cggcaacgcc cgcaccgacc acaatggcat
    17941 caaatttgtc atccgacatg cgttatgctc ccttcccgct gcgtttacag cgggagatgt
    18001 ggttgaaagg gaatcagcga ctcaactggc taatcagggc agggacgact ttgtagatat
    18061 cgcccaccag accgtagtcg gcatagttga agattggcgc atttttatct ttattaatgg
    18121 cgacaatcac ttttgcgccg ttgccgccaa ccatatgctg gatctgcccg gagatcccca
    18181 gcgtcaggta gagatcggat ttcagcaaca cgccggagac accgatataa cgttcacgct
    18241 ccatccagtt ctcgccttcg gcaattggac gtgaacagcc gacttcagca ttcagcaccg
    18301 ccgccagttc gtggaccatt ttcaggtcat cctgcgctgc cagaccacga ccgacgccaa
    18361 ccacacgttt tgctttgctc aggtccacgc tgcttgccgc tttcgcacgg cgttcgcgac
    18421 agagaatttc atgacgcggg gcaacataag gtaccgtttc tgtcggacac tgattagagg
    18481 tatcactggt gcacggttcc tgaacaccgg gcgcaagggt aatgatcgcc agcgggctat
    18541 tgatcttttc ctgtgcgaac gctaacccgc catacatccg gtgttcggcg caaatgtgac
    18601 catcgacaat atccaccgca gtggcatcgt tcaccagcgc cgcattcagt tgcacactta
    18661 accgtgcagc cagagcttta ccgcgtttcg tcgcggccag cagcaacata gaagggtgtt
    18721 tatctttcag cagggcagca atgctttcgg cgtaattttc agtacgttgc agcgcgtcgt
    18781 tttgcgcaag aacataaatg cattttggcc cataaggcat aactgtctgc gcctggtcgg
    18841 tattttgtac aatggcatac acctgttggc cccattgctg agcgccgcca aacagttcag
    18901 cataacgttc aggattatcg ctaaagaccc agacgctgtt taattgactc atcgtttctt
    18961 ctccgtaggc ttcagttcag ggctttcttc agatgttccg ccagctcggc aatggcctcc
    19021 ggcgaatcgt tatcgagaat gatgtgctta cgttctgttt gcggcggtac gcgaatgccg
    19081 gtcagttcag caagtggtgc gctctggctc cagccaatat cacttgcctg ccactgattt
    19141 accggttttt tacccgcgcc gaggatggct ttcatcgaag gaatacgtgg tacgttaata
    19201 tcggaggtga cgcagagcac ggctggaaca gagagttcaa taacttcaac atcatcttca
    19261 agcgtgcgtt caatcaccag tgtattgccc tgacgctgaa tggcactcac tgcattaatc
    19321 accggaagtt gcagaatttc tccgaccagc aagccaacct gctgggcata aaggtcgccg
    19381 gaaccttcac caaagatcag taaatcgaag ccgatctttt caactgctgt cgccagcgcc
    19441 tttgcggtat cgagaggcag tgcatgctca agttgtgcat cctgcaccat atacaggctg
    19501 tgcggcccgc gggatagcac gtctttgcgc actttcgagt tctgcaacaa agagccgccg
    19561 acggtcagcg cggctatctc atcgtcatct gttgcaagct ggcttgcagc ttcaatggca
    19621 ttgagatcga actggctgat tttggcgtcg gcattgtcga aattcagggt atattctgga
    19681 gtgacaacaa tgtcctgttc ttcaggcacc agcttaaagc aggttattat tttcatgatt
    19741 tctcctgtga gtcgcatacg gaaccatctc cgatggaagg taaaaatggc ctgaaaatag
    19801 tgtgcgatag cggtgcgggg ttttcgacgg agcaagctgt tttccttgcc aacaatcttg
    19861 caaaaaaaat gcgatcgcta aaacaaaagt gcaatttgcg gcaatgttgt tattccatga
    19921 ccgtacaatc ttgcattcag tgtaaataat cttgtttagc tctcgctaaa cgcggttgta
    19981 taaccgcaat acacgccgta ttttatccgg catatattgt ttttgcttgc tcatggaata
    20041 gggaggttgt catgtatcaa cgctgttttg ataacgccag cgaaacgctg tttgtcgccg
    20101 gtaaaacgcc acggctttca cgttttgcat ttagcgatga tccaaaatgg gagtccggac
    20161 atcacgttca tgacaatgaa accgagttga tttacgtcaa gaaaggggta gcaaggttta
    20221 ccatcgattc ttcgttatat gtcgcgcatg ccgatgacat tgtggtgata gaacgtggca
    20281 ggttgcatgc ggtggcctct gacgttaacg atccagcaac gacgtgtacc tgtgcgctgt
    20341 acggctttca gtttcagggg gctgaggaaa atcagctact gcaaccgcat tcttgcccgg
    20401 taattgccgc agggcaggga aaagaagtca ttaaaacctt atttaatgag ctaagtgtga
    20461 ttttgccgca aagtaaaaat agccaaacat cttcgttatg ggacgcattt gcctatacgt
    20521 tagcaattct ttactatgaa aactttaaaa atgcttatcg ttcggagcag gggtatatta
    20581 aaaaagatgt tctgataaaa gatattcttt tctatctgaa taataattat cgcgaaaaaa
    20641 tcactttaga gcagttatcg aaaaagtttc gtgccagcgt cagttatatt tgccatgaat
    20701 ttaccaaaga gtatcgcatt tcccctatta actatgttat tcaacggcgt atgacggaag
    20761 cgaaatggtc actcactaat actgaattat cacaggcaga gatctcctgg cgtgtgggtt
    20821 atgaaaatgt cgatcacttt gccaaactgt ttttgcgcca tgtcggctgt tcgcccagcg
    20881 attaccgcag gcaatttaaa aactgttttg cggaacaaga aatcctatct gaatttcctc
    20941 aaccagtaag tcttgccgga taaatctggc ggcgcagttt gttaagaagc tgcgccgcct
    21001 gcagattatt tgttctgata atctttcagg atctgccgac ctgctacgta aatcataatt
    21061 tcgtctgtac cgccgccgat acgttcacaa cggacatcac gccagaagcg ggagacgcgc
    21121 gcctcatcgg tatagcccag accgcccatg atttgaatcg catcatcaat gacttccatt
    21181 gcggtacgtg cgcagtacag ttttgccagc gccgcgctgg tgcgcagtga ctgatgctga
    21241 tcggcttgcc atgccacttt caacaccatg ttgcgcatgt tgtcgatctt aatcgccatc
    21301 agcgccagtt tttcctggat catctggtta tgaccaatgg gcttaccaaa agcgatacgc
    21361 tggttggcat agcgggcggc atcttcgaat gcgcattcgg caaagccggt gctgcgcgcg
    21421 gcgttgatca ggcgctccat ctcaaagttg tacatcacat tgaggaaacc cattccttct
    21481 tcgcccacca tgtcgctctc ttcaacttcc acgttgtcga gatagacttc gcaggtgctg
    21541 agcatatgcc agccgatttt atgcagtgga ttgattttaa tgccgggctt actggagtcg
    21601 acccaccaca gagtaaaggc ctttttcgga tctttcggtt gcggatcgcg cgccaacacc
    21661 agcatatatg gatactcttt cgcaccggta ataaaggttt tctgtccgtt gatgtaaacc
    21721 ttgccgtttt tacgcgtgta agtggtagtg gcactgttgt tgtctgagcc tgcgcctggc
    21781 tctgtcaatg ccagggcata ggcgggatca cccgtttcca gggtgctttc cgccgtttta
    21841 cgtagctgct ctgcagaacc gaaacggcgc atactgtgaa tacattgacc gttggtgatc
    21901 aaaaatgccg gggcaccgca ttttgacact tccatcagcg ccagcatttg ggtgacgtaa
    21961 tccgcaggga taccaccaaa ttcttccggc acgccaagca tggaaatacc gttatccgcc
    22021 agtgcccgca taaattcacg cgggtatgtc ccgttttgat cgcaggtgcg gaaatactct
    22081 tccggaaagt tagtcgtaat cagttcgcga atactggcca gcagcagttc ttgttcttca
    22141 gttaaagaaa aatccatcat tatactcctg ttaatgagcc gcttcaggca ggacaaaacc
    22201 catcgccgca tcgataaata atctttcgtc catcagtttg agttctggcg aaatcactgg
    22261 ggtgaaatcc attttgtcga gaatatcttt ttgtaaatcg acgccaggag cgatttcgat
    22321 taaatgcagg ccgtcttctt tcagcgtgaa tacggcgcgc tcagtgatat aacgaacatc
    22381 cagccctcgc tcgagcgcga tttttccgct aaaagtaatt tctggtagtt cccgaataaa
    22441 tttcttcacc cgtccttcct ggacgatatt taatttgccg tcggtaattt ctgtttttaa
    22501 actgcccgca gttaatgtgc cgcagaaaac gattttcttc gaagtggcac tgatatcaat
    22561 aaatccaccg gtgcccatga ttttaccatt gaatttatgc acgccgacgt taccgtgctg
    22621 gtcgacttca gcaaaactca aataacaaac atccagacca ccaccgtgat aaaaatcaaa
    22681 ctgggacgtc atatccagaa tggcgcgggt attcacgttc gcgccaaagg cgatcccctg
    22741 tgaagtaata ccgccaatcg gacctgtttc taccgtcaga ataaagtcat cagcacaacc
    22801 ttcttcccgg gcgaccaggc caatgccgtc agcaataccg acgccgacat tccccactgc
    22861 gcctttgcgc atttcgaata atgcacgccg tgcaactaat ttacgttggt ttaggggcag
    22921 gctaagtttg gtgctgtcat caagggtgaa gtcaccagaa ataaagcggt taaccggcgc
    22981 tccgccatac agttgggttt gatccggatc gaccaccaca atatccacca gataacccgg
    23041 aatacggaca gatttaggat gcagcgtggc tttcttaacc attttctgca cctgcatcat
    23101 cacaataccg ccgttattgt gcaccgcctg agcaataacc aatgcgtcga gatacatcac
    23161 ctcatcttca aaagtggcgt agccttcgct gtcgcaggtg gtagcgcgaa tgaaggcgat
    23221 atctggcgct atcgctttgt aatagagata ttctttgtta tcaaactcga ccagtttaat
    23281 caggtcttct ttagtgactt cattcagttt gccgccttgc tggcgtggat cgacaaatgt
    23341 tccgatgcca atatcactaa taataccagg ctggtgggct gcggcggcgc gtaaggtttg
    23401 tgtaagtaca ccttgtggat agttataagc aataatttta ttttgttctg cgagatcaga
    23461 aatacgcggc gattgtcccc agtgaccgca taatgcccat ttcaccaggc cttcttgcgc
    23521 cagcggacta ataccacggt cggcgcgatc gccaagccct gttggactaa taatcgataa
    23581 attacgtggt gtttgagtct gtttatattt atcagcaaga gcagtaatta acgtggtggc
    23641 ttccagaata ccgccgccag cgcctaacac acaaagtgtt gcttcgtcgg gaatataatt
    23701 caccgcttcc tgtgccgaca ggaccggcac tcgtccatta atgcgaggtg gttttacagg
    23761 tttcattgtt aattcctgat tcaggtgtga ttttccctac gccagatgga gaattagcgc
    23821 acagagactc acgaaatatt tttattaaat acggttaaaa atactttttt tagttcggcg
    23881 ggggagagta ttcccgccga aatattattg cttatgcctg atgtaaaata gttaataccg
    23941 tacgcaaatc atttaccgag atttgccctg gcgcagaggc tttttttacc gcaccaaaag
    24001 ttgccgccga gccaaatact tcaccagcca gacgagaaat tacgccagtt tttgccatcg
    24061 acatcgtgat gattggacga tcggcatact gctcctgcat ctccagggtc gcagcaagca
    24121 acgtcagcac atcgctggta ctttgcggca tcagcgcaat cttaggaata tcggcatcga
    24181 aggactgcat tttgcgcaga cgggcaatga tttcttcggc ttccggcgtt ttatggaagt
    24241 catggttgga catgacaact ttcacatcat gcgcgtgggc gtaggcgacg gtttctttaa
    24301 cctgatcatc gccggtaaat aactccagat cgattatatc aaccaggccg ctgtcgatgg
    24361 ctgcacgatt gagtgcaata taagcctcgg tggaaatcgc ctgctcgccg ccttctttgg
    24421 cactgcggaa ggtaaacagc agcggttttt ctggcatggt ttcacggaga atttttgctg
    24481 ccgctatgac agactccaca ttggagagat cggcatagtg gtccacacgc cattccagaa
    24541 tatcaaagtc cgcttcacga taggcgagag cttcggattt cacgctggcg atatctttcg
    24601 ccatcagcga gacgatgatt ttaggtgcgc ccgtaccaat gacgagatct tttacagtta
    24661 cggttttcat tttttaccct ttctgcacgc ggtcagcctg tcaggcaccg aaccccatga
    24721 cctgtttaac atattccaga gggaaatctt tgccagtcca taacgtgaac tgttcagccc
    24781 cttgccacaa caacatgccg tatccatcaa tcgttttgca acccgcttgt tgcgcctgct
    24841 gcaataactt cgtcatatga gggttataca cgcattcagt gaccagaagt cccggatgta
    24901 acagactgat atcattaacc aatgattcat tctcaagggg tttcataccc actttggtgc
    24961 cattggttaa aatgtcggcg gaagccaggg cttctgcaaa ggcttgctga tcggcgagat
    25021 cggtgactgt cacgacgcaa tcggtgtttt cgttaacccg ctgcgcgaag gcgagagctt
    25081 tatcgaagaa ctcatcccga cggttaaaga gtttaatttc ttttaaacct tcaattgccc
    25141 cctgcgcgcc aattgccgtt gaggcaccac cggcccctaa tagcaccatc gttttgcctt
    25201 tgatatcaaa accgctctct ttaatggcgc gaatatgacc cgtgccgtcg gtgttatagc
    25261 cacgcagata gccatcatca ttaacgatgg tgttgatggc tcccaccagt ttggccgctg
    25321 gcgttaattc atcaacatat tcacacgcca gttgtttgtt cggcattgat acaccagttc
    25381 cgcgcatttt gagggctttt aatccttcaa ttgctccggg aaaactatcg ttatccactt
    25441 caaaggccat ataggtaaat ggcaatcccg ctttttctaa ggctttattc tgcatttcgg
    25501 gcgataaact gtggcggatg ggataggcca tcaacccaat caattcgtat tttgcggtaa
    25561 catccataat taactccttt aacctctatg cttaatcgtg tttaaccggg tgctaaattt
    25621 atgctcgcca aaccgcacat cattttcagg aataatgaaa acgcggtaat agcggataaa
    25681 aacaataatt gcggtaatca gggccagcag cgcgaaagta aaatcgagaa caatgatata
    25741 ttgcaggccg atgttcgaca gataaccggt aatcagcgga ataacaaagt tagccagtcc
    25801 acccatcatc atatatatac tggtgacttt ggctttgctt tttgggaaaa actctgacat
    25861 gaccgaaacg ccgagctgta aaatgccgcc agctgctgag aaaccgataa caaaggctcc
    25921 ggcattacac accaatggag aagggtacag gtagataatg gcagctgtta ttgttgccag
    25981 tgcagagtta aatacattag cccagatggg ccggaccatt tttttcagca gggcggcaaa
    26041 aataaagaca cagaccagcg aacccatact gtaataagag atggttttta atgcctcagc
    26101 ttctgacata ccagcaaaag ccatcgcata tttgggcatc cacaccacaa tcacataaaa
    26161 ggtcgcgaat gcggctacac cgaacagtac tgatgaaaca ccttccagcc agactaacgg
    26221 tttgctgttc atttgcggta attcattagc tacgctggcg tccaccaact ggctggggaa
    26281 tttgcttttc aacagcatca gcgtgatcag tacaaacaga atacccggaa taatcagccc
    26341 atagccgtac cagatgttat tgagcaacat atagctcacc agcattgggt agaacatttg
    26401 cccgaatgac accatcgctt taaccagtat gaccgccgaa ccagaggctt tcggaaagca
    26461 ttccataagc gcggggtagc cacccgtatc cagcgctgag ttagcgatac ctacgcacac
    26521 tgccagacag taggcgagag ttaaattcgg gcaagcagga ataccaaaga agaatagcag
    26581 atacattatt actgccatta atatcaccgc ccgacgacca aacttatcgg agatcacacc
    26641 gaagaataaa atactgatca atcgccccaa tccgataccg gaaattaaat aggcaatgcc
    26701 cgcgttgtca gtggaaaact tttctgccaa agatgtcatg ttttgggcaa gcgtaataac
    26761 actaatgccg tgcaggaaat agctgaagta aatacaaaga acagccagga taaatggcgt
    26821 gctgaaagcc ttattttgag acattttcac agctccttgc tgaaagataa tttcgttacc
    26881 gtacgtattg ttctccgacg gtttacataa ttactatact gaagataatt ttccctgggt
    26941 acatggcata aagtttatgt ttccaggaat attgcatgtc atagtttgtt tttgatcgcg
    27001 attttcctta gttagcgcag aaagagttat ttatccgtaa gatcttgcat gacctgaaaa
    27061 gaatattgca ttacccaccc ggagcgacat tttcctttag cgagtgatgc cgcgtttctt
    27121 tgcgactgcg taagccaata aacagtgcca gtataaagcc gatggcagcg atggcggtat
    27181 cgaaccacat aatatcggca atacttcttt gcgaaagatg agccgtaatc aacggaatag
    27241 taaaggttgc aatgctgccc gcactgtaat agatccccgt tgcttttcct ttagcgtagg
    27301 gaaaacgttc cgccattaac gtcaggccaa tttgtacaac accgcctgcg gaggtaaaac
    27361 cgatgacgaa agcaaatatt atcaccacat aaaatgtggg atgcaggcag acggtgagca
    27421 gggcgataaa tgagataaag gtgtacagca tcagtaatgt tgttgggcga acggtattac
    27481 gaatgagtgg agcggtaata aatacacaaa gcagcgaacc cacggtgtag atactgagta
    27541 atttgatcga catggtgtat gacatgccgg cgacaaattg tccgtattgt gccagccact
    27601 ggctaaccag ataaaacgtt gccattgaga tatagccata tagggaataa ctggctaaat
    27661 cgataattga acagcgatgt tccgtagagc tggtggtttt ctttatgaca ggtaagtgac
    27721 gacccggatg gggtgggaac gtacaacggt ataaaagcag agcgttaata aacataatgc
    27781 ctgcggcaat cataaaggac caaccgaacc acagttcagc ccacactaac aggctgataa
    27841 ttagcggtaa taaaaattgt ccgctggaaa caaatgcttt aattaaaata ttggctgtac
    27901 ccggtgagcg tggaaaagct tccatcaaac tgggataggt gcctgcatcg agaaaactgt
    27961 ttgccattcc cgccagaaag ccaaaaacat aagcgataat gatgttattg gtgtgcagga
    28021 tgccaaaaaa gaaggccata tagcagcaca tcccgagcat gataaaaggg cggcgaccaa
    28081 agcgatcgga taataatcct gcaaaaagca agacacttaa tcgaccaatg cccagtgatg
    28141 agataactat cgagacgccc gcggcattag tctgccaaag cgtctccagc gaggccatat
    28201 tcaggctcat caaaatgacg cccataccat gcaccaggta attaaaatac aacccaagtg
    28261 cggtagggta atagggattt ttcataggtt ctacttagca aaggtgcttt aaaaagtgta
    28321 gagatgttgt gtcagtatcc gttcattatt gttgtaacaa agacaattcc tttgtgtgga
    28381 gaaattaaca aagccgctcc agatcgtgtg catataattc agcggctacc gattgataga
    28441 tcttccagtc aataaaattg ccatcagggt agacttgttg aaacacggtt aagtcgggga
    28501 aaaaacgcat ggtgttgttg ccgatgcggt tatttatttt ctctatgatg gcatgaagat
    28561 gtttttttcg ttgctgacgc atagccaaca gttgctcgac tacacgatca ggaatggcgc
    28621 atttgccatt ttcccactga cgccagcttt cactgtcatc ggtctgggca atccaggtcg
    28681 cgcattcatc aatagtcatg gcaaaaatat ggcgaagtgc ctggagttca taagcgttca
    28741 tcgtgtaatg gctgcatgaa ataaacaata aaatataaat aacattatca gcctgctgac
    28801 ggcttgaaga gagtcacagt atcttgtgca acattattgg aaagcgtgag gggcgagata
    28861 gcaaagcgga aaaatgttaa aaaaaagccc atcgtgggag atgggcaaag actacacaca
    28921 gcaattcgtt gtttcactca ggggatttcc atgcttataa atcaatatgt tgatttataa
    28981 ccgtgagcta atagtaggca tacggacttt ttgcgtcgat cagattcgtc tcattagttt
    29041 aaaaactgta aagaatttgc gacaggtact accagctgaa tggcggtaag caggaaaata
    29101 tggggaagag caagcaaata tcaattagca ggggttaaca atcaggcggc agagccgcct
    29161 gatgagaaat tacttattcg atttctcgat ttcgccaagt tcttcgagaa tttcttccgg
    29221 ttggtccgtc ggcggtggga cttcttccac ccacgcggca aaaagacgcc aggaaacggc
    29281 taacagaacc ggaccaataa acaggccgat catcccgaaa gcaatcaaac caccaataac
    29341 accagagagg atcaggatca gcggtaaatc ggcacccatg cgaattaaca ttgggcggat
    29401 gacgttatcc agcgtgccaa ccacaccgct ccacactaac aataccgttc cccaggtggt
    29461 atcaccggtc cagtagagcc agataatcgc cggaatcagt accggcaacg gtccaagctg
    29521 gacaaggcag gagaggatca ttaacaccgt gagcaaagtt gcataaggta cgccggatac
    29581 ggcgaggccg ataccgccaa gcactgcctg tactaacgcc gtcaccacca cacccagcgc
    29641 caccgcgcgg atagcctgtg ccgccagtag cacagcagca tcaccgcgaa cgcctgccag
    29701 acgggttgca aaatggcgaa tgccttgtgc cacctgttca ccgcgccagt acagcagggc
    29761 actgaagaga agcatcagcg cacaatgcac cataaagcgc ccgatatgcg ccgcctgccc
    29821 aacgaaccag gtggtggtgg tgccaatata aggacggact ttcgccatga tcgccgtacc
    29881 ccccatatcc agcaagttgt gccagcctgc atatagcttc gcgccaatca ccggaatggt
    29941 attaagccac gccagatcgg gtaacgtcat gtcaccgctg gaaatggctt taattagcgg
    30001 accgctgcca tcgacaatac tgttaaccaa caaggcaata gggatgataa acaccatcac
    30061 taataacagc gtcatcacca gaacggcgag agagcggcgg ccaaacatga tcttttgcaa
    30121 acgtaacaat accggccagg tggcgataac cactgtaccg gcccatgcaa agccgagaat
    30181 aaagggttga acaatccaca gacatgccac aatcatgatg gctaaaaaca gcaccgaaag
    30241 cagaatttgt gcgacatccc tgggctgacg aacatttacc ataactactt ttcacctttt
    30301 ttgtgcgtca aatcgtcgac gcgacgtgaa cacgcgaaac tcacctgcat aatgatgagc
    30361 aatttcagcg gtttttaaca ggtcgattct gcccttaatt ctgatgggcg tgtaagaaaa
    30421 aaatgtggta caacaactca ggcaacacgc aaacgattta ctcgtcgtat ttcaaattgc
    30481 atgtgctgcg gctacgttcg ttcaccccag tcacttactt ttgtaagctc ctggagttca
    30541 ctcgcttgcc gccttcctgc aactcgaaat ccatagagta aacaaacatc acctttcagt
    30601 gcgctattag cagcctgaaa gaacagtgta tcgatctaca tcacagaccg caggaaaggg
    30661 tcaatataat gattccacag atttcccagg cacccggcgt cgttcaattg gtgcttaatt
    30721 ttttgcaaga gctggagcaa cagggtttta ctggcgatac ggcgacaagt tatgccgatc
    30781 gtctgacaat gtcgaccgac aacagtattt accaacttct ccccgatgcg gtggtatttc
    30841 cgcgttcaac cgcagatgtg gcgctgatcg cccgtcttgc cgcgcaggaa cgctattcat
    30901 cgctgatctt taccccccgc ggcggcggca ccggcactaa cggtcaggcg ctcaaccagg
    30961 ggattattgt tgatatgtcc cgccatatga accgcatcat cgaaattaac cctgaagagg
    31021 gctgggtgcg cgttgaggcc ggggtgataa aagatcaact caatcagtac ctgaaaccgt
    31081 tcggctactt ttttgcgccg gaactttcga ccagcaaccg ggcaacgctc ggtgggatga
    31141 tcaataccga tgcatccggt cagggatcgc tggtctatgg caaaacgtca gatcacgtac
    31201 ttggcgtacg cgcggtgttg ttggggggcg atattctcga tacgcaacct ttacccgtcg
    31261 aactggcgga aacgctgggt aaatccaata ccacaatcgg gcgaatttat aacacggttt
    31321 atcaacgttg ccgtcagcaa cgccagttaa tcatcgacaa cttccccaag cttaaccgct
    31381 ttcttaccgg ttacgatctg cgtcatgtct ttaacgatga gatgaccgag ttcgacctga
    31441 cgcgcattct gacgggttca gaagggacgc tggcctttat taccgaagcg cgactggata
    31501 ttacgcgctt gcctaaagtg cgccgtctgg tgaacgtcaa atatgactct tttgactccg
    31561 cactgcgtaa cgcgccgttt atggttgagg cgcgggcgct ttcggtagag acggtggact
    31621 caaaagtgct gaatctggcg cgggaagata ttgtctggca ttccgtcagc gagttgatta
    31681 ccgatgtgcc tgacaaagaa atgctcgggc tgaacattgt ggaatttgct ggtgatgatg
    31741 agacgctgat tgatgagcgg gtaaatgcac tctgtgcgcg gttggatgaa ctgattgcca
    31801 gccaacaggc aggtgtgatt ggctggcagg tgtgccgcga gctggcgggt gttgaacgta
    31861 tctatgcgat gcgcaaaaaa gccgttggtc tgcttggcaa tgccaaaggt gccgctaagc
    31921 caattccgtt tgctgaggat acctgcgtac cgccggaaca tctggccgat tatattgctg
    31981 aatttcgcgc gctgctcgac agccacggct taagctacgg tatgttcggt cacgtcgacg
    32041 caggtgtctt gcacgtccgt cctgcgctgg atatgtgcga tccgcaacaa gagattttga
    32101 tgaagcaaat ctctgatgac gtagtggcgc ttaccgcgaa atacggtggt ttgttgtggg
    32161 gcgaacacgg caaaggtttt cgtgccgaat acagccctgc gtttttcggt gaggaacttt
    32221 ttgcagaact gcgcaaagtg aaagcggcat ttgacccgca taaccgactc aacccaggga
    32281 agatttgccc gccagaaggt ctcgatgcgc cgatgatgaa agtggacgcg gtgaagcgcg
    32341 gtacattcga tcggcagatc cccattgcgg tacgccagca gtggcgcggt gcgatggagt
    32401 gtaacggcaa cggtttatgc ttcaactttg atgcccgtag ttcgatgtgt ccgtcgatga
    32461 agatcaccca gaaccggatt cattcaccga aagggcgcgc aacgctggtg cgtgaatggc
    32521 tgcgcttgtt ggcggatcgc ggcgttgacc cactcaaact ggaacaagaa ctgcctgaat
    32581 ccggcgtcag tttgcggacg ttaattgccc gcacgcgcaa tagctggcat gcgaataaag
    32641 gcgaatacga cttctcacac gaagtcaaag aggcgatgtc gggctgtctt gcctgtaaag
    32701 cgtgttcgac ccagtgcccc atcaaaattg atgtgccgga gtttcgctct cgttttctgc
    32761 agctctatca cacccgttat ttacgcccgc ttcgtgacca cctcgttgct acggtcgaga
    32821 gctacgcacc gctgatggca cgcgcgccga aaacctttaa cttcttcatt aaccagccgc
    32881 tggtgcgcaa gctctcggaa aaacatatcg gcatggttga tttgccactg ctgtcagtcc
    32941 cctcgctaca acaacaaatg gtggggcatc gctcggcgaa tatgacgctg gaacagcttg
    33001 aagctctcaa tgcagagcag aaagcgcgca cggtgttggt ggtgcaggac ccctttacca
    33061 gctattacga tgcgcaagtg gtggcggatt ttgtccgtct ggttgaaaaa ttaggtttcc
    33121 agcctgtgtt actgccattt tcgccaaatg gcaaagccca gcatatcaaa ggctttctta
    33181 atcgttttgc gaagacggcg aaaaagacgg cggatttcct caaccgtatg gcgaagctgg
    33241 ggatgccaat ggtgggcgtc gatccggcgc tggtactttg ttatcgcgat gaatataaac
    33301 tggcgctggg cgaggaacgt ggcgagttta acgtcttact ggcgaatgaa tggctggcaa
    33361 gcgcacttga ctcacagcca gtggctacag tcagcggtga atcatggtat ttctttggtc
    33421 actgtaccga agttaccgct ttgccgggcg cgccagcaca atgggttgcg atatttgccc
    33481 gttttggcgc gaaactggaa aatgtcagcg tggggtgctg cggcatggca gggacttacg
    33541 gacatgaagc gaaaaaccat aaaaattcgc tcgggatcta tgagttatcc tggcatcagg
    33601 cgatgcagcg actgccgcgt aatcgctgtc tggcgaccgg atattcctgc cgtagccagg
    33661 taaaacgggt cgaaggcacg ggggtacgcc atcctgtgca ggctttactg gagattatta
    33721 aatgatatgg aaacgaaaaa tcaccctgga agcactgaat gccatgggtg aaggaaacat
    33781 ggtggggttg ctggatattc gctttgaaca tattggtgat gacacccttg aagcgacaat
    33841 gccagtagac tcgcggacaa agcagccttt cgggttgctg catggagggg catccgtggt
    33901 actggccgaa agtatcggtt ccgttgccgg ttatttatgt accgaaggtg agcaaaaggt
    33961 ggttggtctg gaaatcaatg ctaaccacgt ccgctcggca cgagaagggc gggtgcgcgg
    34021 cgtatgcaaa ccgttgcatc tcggttcgcg tcaccaggtc tggcagattg aaatcttcga
    34081 tgagaaaggg cgtttgtgct gttcgtcacg attaacgacc gccattttgt gacgttgtca
    34141 ccagaaaagt gtgacgcata ttgcttttgg tcattatctg cacagtaaac ggtggtatac
    34201 tgtgcaggtc ttgtctaaaa gcgaggatgc tatgtctact caacttgatc ccacccagct
    34261 ggccattgaa tttttacgcc gtgaccagag caatctttca cctgcccaat atctcaaacg
    34321 tctgaaacaa ctagaacttg aatttgctga cctgcttact ctctcttctg ctgaattaaa
    34381 agaagaaatc tactttgcct ggcgtctggg cgtgcattga tcctgactta atgccgcaat
    34441 tttgccaaat cgacctggat taacctccgt ccaggttcga tgaccttctt aatactgctt
    34501 atctatccgc ctgatattgt tcaaaaataa tactaatcgc agtttgtgtt aaaacggcgg
    34561 gttagcttta tgagattatt cttatcgccc cttcaagagc taagccactg tgagtgccgg
    34621 agataagcgc cggatggggt agaaaccctt aagcctgtgt cgcacagact taagggtttt
    34681 cttatttctg aacgctgcac ttttttacct atcccttaaa tagtcatttt caatacaact
    34741 ttttcttttt catcacagcc ttaatgctcc ttagataata atcattatct aacaatgaga
    34801 tacctaattc ttagcgtgcc tgttaaccca cacatcaggg tctatgctta ttaaaagcag
    34861 gtaaaaagca tgttttacgc taaagtccct gcgtttgctg ggttgaactg ataatcatta
    34921 tcactaacat gctgttatac gctgaaagcg atgaagtgag gtaaatcgat ggacatgcat
    34981 tcaggaacct ttaatccaca ggatttcgcc tggcagggct taacgctgac acccgcagcc
    35041 gctgtccaca tccgcgagct ggtggcaaag cagccgggta tggtcggcgt gcgcttaggc
    35101 gtgaagcaaa cgggctgcgc gggctttggc tatgtgctcg acagtgttag cgagccggac
    35161 aaagacgatc tgctgtttga acacgacggc gcgaagctgt ttgtcccgct gcaagcgatg
    35221 ccgtttattg atggcacgga agtcgatttc gttcgtgaag gacttaatca gatattcaaa
    35281 tttcacaacc ctaaagccca gaatgaatgt ggctgtggcg aaagctttgg ggtataggcg
    35341 gtactatgtc tcgtaatact gaagcaactg acgatgtcaa aacctggacc ggcggcccgc
    35401 tgaattataa agaaggattc ttcacccagt tagccaccga tgagctggca aaggggataa
    35461 acgaagaggt ggtgcgcgca atttcggcga agcgtaatga gccggagtgg atgctggagt
    35521 ttcgtctaaa tgcctatcgc gcatggctgg agatggaaga accgcactgg ttgaaagcgc
    35581 actacgacaa gctgaattat caggattaca gctactattc ggcaccatcg tgcggtaatt
    35641 gtgacgacac ttgtgcgtct gaacccggcg cggtgcagca aactggcgcg aacgcctttt
    35701 taagtaaaga ggtggaggcg gcgtttgagc agttgggcgt tcccgtgcgg gaaggcaaag
    35761 aggtggcagt ggatgccatt ttcgactctg tttcggttgc cactacttat cgcgaaaaac
    35821 tggcggagca gggaattatt ttttgttcct ttggcgaggc gatccacgat cacccggaac
    35881 tggtgcgtaa atatctcggc accgtggtgc cggggaatga caacttcttt gccgcactta
    35941 atgcggcggt agcctctgac ggtacgttta tttatgtgcc taaaggcgtg cgttgcccga
    36001 tggaactttc cacctatttt cgcattaacg cggaaaaaac cgggcagttt gagcgcacca
    36061 ttctggtggc cgacgaagac agctacgtta gctacattga aggctgttcc gctccggtgc
    36121 gtgacagcta tcagttacac gcggcggtgg tggaagtcat catccataaa aacgccgagg
    36181 tgaaatattc cacggtacaa aactggttcc ctggcgataa caacaccggc ggtattctca
    36241 acttcgtcac caagcgtgcg ttgtgcgaag gcgaaaacag caaaatgtca tggacgcaat
    36301 cagaaaccgg gtcagcgatt acgtggaaat atcccagctg cattttgcgc ggcgataact
    36361 ccattggtga gttttactca gtggcgctga ccagcggtca tcagcaagcg gataccggca
    36421 ccaagatgat ccacatcggt aaaaacacca aatcgaccat tatctcgaaa gggatctctg
    36481 ccggacatag tcagaacagt tatcgcggct tagtgaaaat catgccgacg gcaaccaatg
    36541 cgcgcaattt cactcagtgc gattcaatgc tgattggcgc taattgtggg gcgcatacct
    36601 tcccgtatgt cgaatgtcgc aataacagcg ctcagctgga gcacgaggca acgacatcac
    36661 gtattggtga agatcaactg ttttactgcc tgcaacgcgg gatcagcgaa gaagatgcca
    36721 tctcgatgat tgttaacggt ttttgcaaag acgtgttctc ggaactgccg ctggaatttg
    36781 ccgttgaagc acaaaaactc ctcgccatca gtcttgaaca cagcgtcgga taaggaataa
    36841 acatgttaag tattaaagat ttacacgtca gcgtggaaga taaagctatc ctgcgtggat
    36901 taagcctcga cgttcgtccc ggcgaagttc acgccattat ggggccaaac ggttctggca
    36961 aaagtacctt attggcaacg cttgccgggc gagaagatta tgaggtgacg ggcggcacgg
    37021 ttgagttcaa aggcaaagat ttgcttgcgc tgtcaccgga agatcgtgca ggtgaaggca
    37081 tctttatggc cttccagtat ccggtggaaa ttcccggcgt cagtaaccag tttttcctgc
    37141 aaacggcact taatgcggtg cgcagctatc gcggccagga aacgctcgac cgctttgatt
    37201 ttcaggattt gatggaagag aaaatcgctc tcctgaagat gccggaagat ttattaaccc
    37261 gttcggtaaa cgttggtttt tccggcggtg agaaaaagcg caacgatatt ttgcaaatgg
    37321 cggtgctgga accggagtta tgcattcttg atgagtcaga ctccgggctg gatattgacg
    37381 cattaaaagt ggttgccgat ggcgtgaact cgctgcgtga cggcaagcgt tcattcatca
    37441 ttgttacgca ctaccaacgc attctcgact acatcaagcc tgattacgtc catgtgctgt
    37501 atcagggacg aattgtgaaa tccggcgatt tcacgttggt caaacaactg gaggagcagg
    37561 gttatggctg gcttaccgaa cagcagtaac gcactgcaac agtggcatca tttgtttgaa
    37621 gctgaagggg cgaaacgctc cccgcaagca cagcagcatt tacaacaatt gctgcgtacc
    37681 ggactgccga cacgtaaaca tgaaaactgg aaatatacgc cgctggacgg gctgaccaat
    37741 agccagtttg tcagcattgc gggggagata tccccacagc agcgtgatgc cttagcgtta
    37801 acattagacg ccgtgcggct ggtgtttgtc gatggacgtt acgtgcccgc actgagcgat
    37861 gcaactgaag gcagcggata tgaagtgagc attaacgacg accgtcaggg gttacccgat
    37921 gctattcagg cggaagtgtt tctgcatttg acggaaagcc tggcacaaag cgtgacgcat
    37981 atcgccgtga agcgcggtca acggccggca aagccattgc tgttaatgca tatcacccag
    38041 ggcgtggcag gtgaagaggt gaatactgcc cattaccggc atcatctgga tctggcggaa
    38101 ggtgccgaag caacggtgat cgaacatttt gtcagcctta atgatgctcg tcacttcacc
    38161 ggcgcacggt tcactatcaa cgtcgcagcg aacgcccatt tgcagcatat caagctggcg
    38221 tttgaaaacc cgctcagtca ccactttgct cataacgatt tgttgctggc tgacgatgcc
    38281 accgcattta gccacagttt cctgctgggt ggcgcagtat tacgacacaa caccagtacg
    38341 caactcaatg gcgaaaacag cacgctgcgg atcaatagcc tggcgatgcc ggtgaaaaac
    38401 gaggtgtgtg atactcgtac ctggttggaa cacaataaag gtttttgtaa cagccgacag
    38461 ttgcacaaaa ctatcgtcag cgacaaaggc cgcgcggtat ttaacggttt gatcaacgtc
    38521 gcgcagcacg ccatcaaaac ggatggtcag atgaccaaca ataatctgct gatgggcaaa
    38581 ctggcggaag tggatacgaa accgcagctg gaaatctatg cagatgatgt gaaatgcagc
    38641 cacggcgcga cggtggggcg tattgatgac gaacagatgt tttatctgcg ctcgcgcggg
    38701 atcaatcagc aagatgccca gcagatgatc atttacgcct ttgctgccga actgacggaa
    38761 gcactgcgtg atgaggggct taaacagcag gtgctggccc gaatcggtca acgactgcca
    38821 ggaggtgcaa gatgactttt tccgtcgaca aagtgcgggc cgactttccg gtgctttctc
    38881 gtgaggtaaa cggtttgccg ctggcttatc tcgacagcgc cgccagtgcg cagaaaccga
    38941 gccaggtgat tgatgccgag gctgagtttt atcgtcatgg ctacgcggcg gtgcatcgcg
    39001 gtattcatac cttaagcgcc caggcgaccg agaaaatgga gaacgtgcgc aagcgggcat
    39061 cgctgtttat taatgcccgt tcggcggaag agctggtgtt cgtccgcggc acgacggaag
    39121 ggatcaatct ggtcgccaat agctggggca acagcaatgt gcgagcgggc gataacatca
    39181 tcatcagtca gatggagcac cacgctaaca ttgtcccctg gcagatgctt tgtgcacgcg
    39241 ttggcgcaga gctgcgtgtg atcccgctca accccgacgg tactctgcaa ctggagacgc
    39301 tgcctacgct gtttgatgag aaaactcgtc tgctggcaat tactcatgtc tccaacgtgc
    39361 ttgggacaga aaatccgctg gcggagatga tcacacttgc gcatcaacat ggcgcaaaag
    39421 tgctggtgga tggcgctcag gcggtgatgc atcatccggt ggatgttcag gcgctggatt
    39481 gcgattttta cgtgttctcc gggcataaac tgtatggccc taccggaatt ggcattcttt
    39541 atgtcaaaga agccttgttg caggagatgc cgccgtggga agggggcggt tctatgatcg
    39601 ccaccgtcag cctgagtgaa ggcactacct ggaccaaagc accatggcgg tttgaagccg
    39661 gtacacccaa taccggaggc atcattggtc ttggcgcggc gctggagtat gtttcggcgc
    39721 tggggcttaa taacatagcc gagtatgaac agaatctgat gcattacgcg ttatcacagt
    39781 tggaatctgt accggatctc actctctatg gcccacaaaa caggcttggc gttattgctt
    39841 ttaatctcgg taaacaccac gcctatgatg ttggcagttt tctcgataat tacggcattg
    39901 ctgtgcgtac cggacatcac tgcgcaatgc cattaatggc ctattacaac gtccctgcga
    39961 tgtgtcgggc atcgctggcc atgtataaca cccatgaaga agtggatcgt ctggtgaccg
    40021 gcctgcaacg tattcaccgt ctgctgggat aacagggagg cactatggct ttattgccgg
    40081 ataaagaaaa attgctgcgt aattttttac gctgcgccaa ctgggaagag aaatatctct
    40141 acattattga gttgggtcag cgtctgccag aattacgtga cgaagacaaa agcccgcaaa
    40201 atagcatcca gggctgccag agtcaggtgt ggattgtgat gcgtcaaaat gcgcagggaa
    40261 ttattgaatt acagggcgac agcgatgcgg ccattgtaaa agggcttatt gcggtcgtct
    40321 ttattcttta cgatcaaatg acgccgcagg atattgtcaa tttcgatgtg cgcccgtggt
    40381 ttgaaaaaat ggcgctcacc caacatctca ccccatcacg ctcacaaggt ctggaagcga
    40441 tgattcgcgc aattcgcgcc aaagccgctg cacttagcta aactaaaggg acagctttca
    40501 tccggttggc tcacattgcc gggtggtcaa tgtttacact ggcctgcaca ggagtgtttt
    40561 ggtttcaggt gaacataagc gccatccgac gtttcagcgt gagtctccgg caaatacagg
    40621 aggtttacaa tgaaatgcgc gtctttgctt acactcacgc ttatcggcgc ttttagcgcc
    40681 atccaggctg cctgggcggt tgattatccg ctaccaccaa ccggcagtcg actggttggg
    40741 caaaatcaaa cgtatacggt gcaagaaggg gataaaaacc ttcaggccat cgcccgacgt
    40801 tttgatactg cggcaatgtt gatccttgaa gccaataaca ctatcgcccc ggtgccaaaa
    40861 cccggtacga cgataactat tccttcacaa ctattattac ctgatgcgcc gcgtcagggg
    40921 attattgtaa accttgcaga gctgcgcctt tataattatc cgccgggaga aaatatcgtg
    40981 caggtttatc cgataggtat tggtttgcag gggctggaaa cgccggtgat ggaaacgcgt
    41041 gtggggcaga aaatccctaa cccaacctgg acgcctacgg cgggcattcg tcaacgttcg
    41101 ctggaacgtg gcattaaatt accaccagtc gttcctgccg ggcccaataa cccactagga
    41161 cgttacgcac tgcgcctcgc gcatggtaat ggcgaatacc tcattcacgg caccagtgcg
    41221 ccggacagcg tcggtttgcg cgtcagttca gggtgtattc gcatgaatgc accggatatt
    41281 aaagccttat tctccagcgt gcggacggga acgccggtga aagtgatcaa cgaaccggtg
    41341 aaatattccg tggagcctaa cgggatgcgt tatgttgaag tacatcgacc actatcggca
    41401 gaagaacagc agaacgttca gacaatgcca tacacactgc cagcaggctt tacgcaattt
    41461 aaagacaata agggtgtaga tcagaaatta gtcgataaag cgttgtatcg tcgggcaggg
    41521 tatccggttg cggtgagcag tggagcaact cccgcagcca gcaatgcgcc ttcagtagag
    41581 tcagcgcaga atggtgaacc agagcaaggg aatatgttac gcgcgacgca gtagcggtaa
    41641 atggcaggca aaaaaaatgg cgcacaatgt gcgccatttt tcacttcaca ggtactatta
    41701 cttgcggtat ttagtagcca tgttgtccag acgctggtta gcacgagctg cgtcatcttt
    41761 agcagcctga acgtcggaac gcattgcgtt cacgtcgttg ctcagctggt caactttagc
    41821 gttcagagtc tgaacgtcag aagacagctg atcgatttta gcgttgctgg agcaacctgc
    41881 cagcagagta gaacccagga ttaccgcgcc cagtaccagt ttagtagctt tcattattaa
    41941 taccctctag attgagttaa tctccatgta gcgttacaag tattacacaa agttttttat
    42001 gttgagaata tttttttgat gggaaggcac ttatttttga tcgttcgctc aaagaagcat
    42061 cgaacgctgg gttttagata aaaaaattga gagaaaacag gatgcttcca tcggat
//
LOCUS       DAZGQL010000034        41912 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.034, whole genome shotgun sequence.
ACCESSION   DAZGQL010000034 DAZGQL010000000
VERSION     DAZGQL010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41912
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.034"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            161..1060
                     /gene="lysO"
                     /locus_tag="ACHKIR_002098"
     CDS             161..1060
                     /gene="lysO"
                     /locus_tag="ACHKIR_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HFO9989841.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQKAALRVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHHQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVASSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            1204..2856
                     /gene="hcp"
                     /locus_tag="ACHKIR_002099"
                     /gene_synonym="priS"
     CDS             1204..2856
                     /gene="hcp"
                     /locus_tag="ACHKIR_002099"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HFO9989842.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            2868..3836
                     /gene="hcr"
                     /locus_tag="ACHKIR_002100"
     CDS             2868..3836
                     /gene="hcr"
                     /locus_tag="ACHKIR_002100"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HFO9989843.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVQVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            3969..5687
                     /gene="poxB"
                     /locus_tag="ACHKIR_002101"
     CDS             3969..5687
                     /gene="poxB"
                     /locus_tag="ACHKIR_002101"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0052737 - pyruvate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HFO9989844.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            5724..6725
                     /gene="ltaE"
                     /locus_tag="ACHKIR_002102"
     CDS             5724..6725
                     /gene="ltaE"
                     /locus_tag="ACHKIR_002102"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HFO9989845.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMTGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            6736..8166
                     /gene="ybjT"
                     /locus_tag="ACHKIR_002103"
     CDS             6736..8166
                     /gene="ybjT"
                     /locus_tag="ACHKIR_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HFO9989846.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPAHEQSDHLRARQATADILREAGVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRRTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKA
                     SASLAALWQVVNQIGGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVD
                     SWKVIVVEPEKQLTLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKRIARLAEQSTD"
     gene            8265..9278
                     /gene="ybjS"
                     /locus_tag="ACHKIR_002104"
     CDS             8265..9278
                     /gene="ybjS"
                     /locus_tag="ACHKIR_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFO9989847.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(9275..10105)
                     /gene="amiD"
                     /locus_tag="ACHKIR_002105"
     CDS             complement(9275..10105)
                     /gene="amiD"
                     /locus_tag="ACHKIR_002105"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HFO9989848.1"
                     /translation="MRRVFWLVAVALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSSGVKYFAPFEQAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTLVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(10102..10425)
                     /gene="ybjQ"
                     /locus_tag="ACHKIR_002106"
     CDS             complement(10102..10425)
                     /gene="ybjQ"
                     /locus_tag="ACHKIR_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFO9989849.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGDQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            10551..11066
                     /gene="ybjP"
                     /locus_tag="ACHKIR_002107"
     CDS             10551..11066
                     /gene="ybjP"
                     /locus_tag="ACHKIR_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO9989850.1"
                     /translation="MRYSKLTMLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRGNDITALRPYLSDKLATLLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            11284..12012
                     /gene="artP"
                     /locus_tag="ACHKIR_002108"
     CDS             11284..12012
                     /gene="artP"
                     /locus_tag="ACHKIR_002108"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HFO9989851.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            12030..12761
                     /gene="artI"
                     /locus_tag="ACHKIR_002109"
     CDS             12030..12761
                     /gene="artI"
                     /locus_tag="ACHKIR_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtI"
                     /protein_id="HFO9989852.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            12768..13484
                     /gene="artQ"
                     /locus_tag="ACHKIR_002110"
     CDS             12768..13484
                     /gene="artQ"
                     /locus_tag="ACHKIR_002110"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HFO9989853.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTVLRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            13484..14152
                     /gene="artM"
                     /locus_tag="ACHKIR_002111"
     CDS             13484..14152
                     /gene="artM"
                     /locus_tag="ACHKIR_002111"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HFO9989854.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            14378..15109
                     /gene="artJ"
                     /locus_tag="ACHKIR_002112"
     CDS             14378..15109
                     /gene="artJ"
                     /locus_tag="ACHKIR_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtJ"
                     /protein_id="HFO9989855.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(15138..16265)
                     /gene="rlmC"
                     /locus_tag="ACHKIR_002113"
     CDS             complement(15138..16265)
                     /gene="rlmC"
                     /locus_tag="ACHKIR_002113"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HFO9989856.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLSVKHMWDLFCGVGGFGLHCAMPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYCIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(16306..16794)
                     /gene="ybjO"
                     /locus_tag="ACHKIR_002114"
     CDS             complement(16306..16794)
                     /gene="ybjO"
                     /locus_tag="ACHKIR_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HFO9989857.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            complement(16854..17699)
                     /gene="potI"
                     /locus_tag="ACHKIR_002115"
     CDS             complement(16854..17699)
                     /gene="potI"
                     /locus_tag="ACHKIR_002115"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HFO9989858.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDNAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            complement(17696..18649)
                     /gene="potH"
                     /locus_tag="ACHKIR_002116"
     CDS             complement(17696..18649)
                     /gene="potH"
                     /locus_tag="ACHKIR_002116"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HFO9989859.1"
                     /translation="MSTLEPAAQPKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            complement(18659..19792)
                     /gene="potG"
                     /locus_tag="ACHKIR_002117"
     CDS             complement(18659..19792)
                     /gene="potG"
                     /locus_tag="ACHKIR_002117"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HFO9989860.1"
                     /translation="MNDAIPRPQAKTRKVLTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLYSPGLV
                     HPLKVDVDASVVDNVPVHVALRPEKIMLCEEPPADGFNFAVGEVMHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRYRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            complement(19887..20999)
                     /gene="potF"
                     /locus_tag="ACHKIR_002118"
     CDS             complement(19887..20999)
                     /gene="potF"
                     /locus_tag="ACHKIR_002118"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HFO9989861.1"
                     /translation="MTALNKKWLSGLVTGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRDNPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            complement(21350..21826)
                     /gene="ybjN"
                     /locus_tag="ACHKIR_002119"
     CDS             complement(21350..21826)
                     /gene="ybjN"
                     /locus_tag="ACHKIR_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HFO9989862.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            complement(21914..22816)
                     /gene="rimK"
                     /locus_tag="ACHKIR_002120"
     CDS             complement(21914..22816)
                     /gene="rimK"
                     /locus_tag="ACHKIR_002120"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HFO9989863.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            complement(22877..23599)
                     /gene="nfsA"
                     /locus_tag="ACHKIR_002121"
     CDS             complement(22877..23599)
                     /gene="nfsA"
                     /locus_tag="ACHKIR_002121"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HFO9989864.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQREAIINSARATSSSSFLQ
                     CSSIIRITDKALRDELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTELLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENNYQPLDKDALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            complement(23583..23870)
                     /gene="ybjC"
                     /locus_tag="ACHKIR_002122"
     CDS             complement(23583..23870)
                     /gene="ybjC"
                     /locus_tag="ACHKIR_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HFO9989865.1"
                     /translation="MRAIGKLPKSVLILEFIGMMLLAVALLSVSGSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            24030..24287
                     /gene="grxA"
                     /locus_tag="ACHKIR_002123"
     CDS             24030..24287
                     /gene="grxA"
                     /locus_tag="ACHKIR_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HFO9989866.1"
                     /translation="MQTVIFGRPGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            complement(24317..24694)
                     /gene="ybjM"
                     /locus_tag="ACHKIR_002124"
     CDS             complement(24317..24694)
                     /gene="ybjM"
                     /locus_tag="ACHKIR_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HFO9989867.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSHRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            24964..26649
                     /gene="ybjL"
                     /locus_tag="ACHKIR_002125"
     CDS             24964..26649
                     /gene="ybjL"
                     /locus_tag="ACHKIR_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HFO9989868.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            complement(26825..27361)
                     /gene="rcdA"
                     /locus_tag="ACHKIR_002126"
     CDS             complement(26825..27361)
                     /gene="rcdA"
                     /locus_tag="ACHKIR_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HFO9989869.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAPGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            27445..28653
                     /gene="ybjJ"
                     /locus_tag="ACHKIR_002127"
     CDS             27445..28653
                     /gene="ybjJ"
                     /locus_tag="ACHKIR_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9989870.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGVPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            28653..29468
                     /gene="ybjI"
                     /locus_tag="ACHKIR_002128"
     CDS             28653..29468
                     /gene="ybjI"
                     /locus_tag="ACHKIR_002128"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HFO9989871.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFSTVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFNQ"
     gene            29553..29837
                     /gene="ybjH"
                     /locus_tag="ACHKIR_002129"
     CDS             29553..29837
                     /gene="ybjH"
                     /locus_tag="ACHKIR_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbjH"
                     /protein_id="HFO9989872.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(29878..31110)
                     /gene="mdfA"
                     /locus_tag="ACHKIR_002130"
     CDS             complement(29878..31110)
                     /gene="mdfA"
                     /locus_tag="ACHKIR_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HFO9989873.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTLLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETATRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLA
                     RLTSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGL
                     VRLTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHVWLNGGNGLFNLFNLVNGILWL
                     LLMVIFLKDKQMGNSHEG"
     gene            31395..31991
                     /gene="ybjG"
                     /locus_tag="ACHKIR_002131"
     CDS             31395..31991
                     /gene="ybjG"
                     /locus_tag="ACHKIR_002131"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFO9989874.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWSMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRFCFALPIRKGWVRD"
     gene            32049..32807
                     /gene="deoR"
                     /locus_tag="ACHKIR_002132"
     CDS             32049..32807
                     /gene="deoR"
                     /locus_tag="ACHKIR_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HFO9989875.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTQRIKLMY"
     gene            complement(32854..34056)
                     /gene="dacC"
                     /locus_tag="ACHKIR_002133"
     CDS             complement(32854..34056)
                     /gene="dacC"
                     /locus_tag="ACHKIR_002133"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFO9989876.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            34303..34929
                     /gene="gstB"
                     /locus_tag="ACHKIR_002134"
     CDS             34303..34929
                     /gene="gstB"
                     /locus_tag="ACHKIR_002134"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HFO9989877.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(34926..36041)
                     /gene="yliI"
                     /locus_tag="ACHKIR_002135"
     CDS             complement(34926..36041)
                     /gene="yliI"
                     /locus_tag="ACHKIR_002135"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HFO9989878.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRMVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDLGEIPDDNPFIKESDARAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(36152..36535)
                     /gene="bssR"
                     /locus_tag="ACHKIR_002136"
     CDS             complement(36152..36535)
                     /gene="bssR"
                     /locus_tag="ACHKIR_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HFO9989879.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            36748..38073
                     /gene="rimO"
                     /locus_tag="ACHKIR_002137"
     CDS             36748..38073
                     /gene="rimO"
                     /locus_tag="ACHKIR_002137"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HFO9989880.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            38299..38685
                     /locus_tag="ACHKIR_002138"
     CDS             38299..38685
                     /locus_tag="ACHKIR_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO9989881.1"
                     /translation="MGVYKARRFSQSTKKLGIHDKVLMAAAEEVIQGIWEADLGSGVI
                     KKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSGLSSKGTKEIEDDELAAYKKMAN
                     AFLAFSNKQIEDLIETGFLIEVKNER"
     gene            38675..39004
                     /locus_tag="ACHKIR_002139"
     CDS             38675..39004
                     /locus_tag="ACHKIR_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO9989882.1"
                     /translation="MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAK
                     VKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSKWERNEIKPSGPALRILNTLAIK
                     GPEVFAK"
     gene            complement(39074..40402)
                     /gene="dgcI"
                     /locus_tag="ACHKIR_002140"
     CDS             complement(39074..40402)
                     /gene="dgcI"
                     /locus_tag="ACHKIR_002140"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFO9989883.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKSVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYFQVCTLIIFFAALVAVISGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQLPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(40410..41912)
                     /locus_tag="ACHKIR_002141"
                     /pseudo
     CDS             complement(40410..41912)
                     /locus_tag="ACHKIR_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic diguanylate phosphodiesterase"
BASE COUNT        10349 a        10864 c        10436 g        10263 t
ORIGIN      
        1 gcacatgaaa ggagcgcaac aatgctaaac actcatatag aattaaaaat ctccctctga
       61 tgcataacat ttccttttaa ttgcaacttt actcgtcctt ccgctttcag tagtgtgtca
      121 tcgcgcattt cgacttcatc tttgcaagga ctgggttttc atgttttctg ggctgttaat
      181 cattctggtt cccctgattg tgggttacct cattccgctt cgccaaaaag ctgcattaag
      241 agttattaat cagctattaa gctggatggt ttaccttatt ctctttttta tgggtatcag
      301 tctggcgttt ctcgataacc tcgccagtaa cctgttggcg attctgcatt attctgccgt
      361 cagtattacc gttattttac tgtgtaatat tgccgccctg atgtggctgg agcgcggcct
      421 gccatggcgc aaccaccatc atcaagaaaa actcccgtcg cgtattgcga tggcgctgga
      481 gtcgctaaaa ctgtgcggcg tagtagtgat tggttttgcc attggtctga gtggactggc
      541 tttcttacaa cacgcgaccg aagccagtga atacacctta attctgctac ttttcctcgt
      601 cggtattcag ttgcgcaata atggcatgac cttaaagcag attgtcctta atcgccgggg
      661 aatgattgtc gccgtggtgg tggttgccag ttcattaatt ggtggtttaa ttaacgcctt
      721 tattcttgat cttcccatca ataccgcgct ggcaatggcc tccggtttcg gctggtattc
      781 tctttccggt attttattga ccgaatcttt tggtccggta atcgggagcg cggcgttttt
      841 taatgatctg gcccgtgagc tgattgctat tatgttaatc cctgggctga ttcgccgtag
      901 ccgctctact gcactgggct tatgcggtgc gacgtcgatg gatttcaccc tgcccgttct
      961 tcaacgtaca ggcgggctgg atatggtccc ggcggcgatt gttcacggtt ttattcttag
     1021 tctgttagtg ccgattctca tcgccttttt ctctgcataa tacctctctg gcggtagatc
     1081 tctgccgcca aaattgcgct aaatcaatct cccttaaagt tgcattaaaa atctctttta
     1141 tccccgcgtt aagcgtctta accttaaaca tgtatattaa atataacttt aaaaggtgtg
     1201 atcatgtttt gtgtgcaatg tgaacaaact atccgtactc cggcaggaaa cggctgctca
     1261 tacgcgcagg ggatgtgtgg taaaacggca gaaacctctg accttcagga tttactcatc
     1321 gcggcgctgc aagggctttc ggcctgggca gtgaaagcac gtgaatacgg cattatcaac
     1381 cacgatgtag acagctttgc gccacgtgcc tttttctcaa ccctgaccaa cgttaacttc
     1441 gattctccgc gtattgtcgg ctacgctcgt gaagcgattg ccctgcgcga ggcgctgaaa
     1501 gcacaatgcc tggctgtaga tgccaacgcc cgcgtcgata acccgatggc tgacctgcaa
     1561 ctggtgagcg acgatctcgg cgagctgcaa cgtcaggcag cagaatttac ccctaacaaa
     1621 gataaagcgg cgattggcga aaacattctc ggcctgcgtc tgctgtgcct gtatggcctg
     1681 aaaggtgcgg cggcctatat ggaacacgcg catgttctcg gtcaatacga caacgatatt
     1741 tatgcccagt accataaaat catggcgtgg ctggggacct ggcctgccga tatgaacgcg
     1801 cttcttgagt gttcaatgga aatcggccag atgaacttca aagtgatgag cattctggac
     1861 gcaggcgaaa ccggtaaata cggtcacccg acgccaactc aggtcaacgt caaagcgacg
     1921 gcgggtaaat gcattctgat ttccggtcac gatctcaaag atctctacaa cctgctggaa
     1981 cagaccgaag gcacgggcgt taatgtttac acccacggcg aaatgctgcc tgcgcatggc
     2041 tacccggagt tgcgtaaatt caagcatctg gtcggtaact acggcagcgg ctggcagaat
     2101 cagcaagtgg agttcgctcg tttcccaggc cccatcgtga tgacctcgaa ctgcatcatc
     2161 gacccaaccg taggcgctta tgacgatcgt atctggaccc gcagcatcgt tggctggccg
     2221 ggtgtgcgtc atctggatgg cgaagatttc tctgcagtca ttgcccaggc gcaacagatg
     2281 gcgggcttcc cgtacagcga aattccgcac ctgatcaccg tgggctttgg tcgccagaca
     2341 ctgcttggcg cagccgatac gctgattgat ctggtgagcc gtgaaaaact gcgtcatatc
     2401 ttcctgcttg gcggctgtga cggcgcacgc ggcgagcgtc actatttcac cgatttcgcc
     2461 accagcgtgc cggatgactg cctgatcctg accctcgctt gcggtaaata tcgctttaat
     2521 aaacttgagt ttggcgatat cgaaggtctg ccgcgtctgg tagatgctgg tcaatgtaac
     2581 gatgcttact cagcaattat tctggcggta acgctggcag agaaactggg ctgcggagtc
     2641 aacgatctgc cgctgtcgct ggtgctctcc tggtttgaac agaaagcgat tgtcattctg
     2701 ctgacgctgc tttctctggg tgtgaaaaat atcgtcaccg gcccgactgc acctggtttc
     2761 ctgacaccag acctgctggc ggtgctgaac gagaaattcg gcctgcgttc tatcaccact
     2821 gttgaagaag acatgaagca actgttgagc gcgtaaggag tttgtcgatg acgatgccaa
     2881 cgaatcaatg cccgtggcgg atgcaggttc atcacattac gcaagaaacg ccggatgtgt
     2941 ggacgatttc cctgatttgc cacgattact acccatatcg cgccgggcaa tatgcactgg
     3001 tcagcgtgcg taactcagcg gaaacgctgc gtgcttacac catttcctcc acgccaggcg
     3061 tgagtgaata catcaccctg actgtgcggc ggattgatga cggtgtcggc tcccagtggc
     3121 tgacgcgcga tgtaaaacgc ggtgattatc tctggctttc ggacgcgatg ggggaattta
     3181 cctgcgatga taaagcagaa gataaattcc tgttgctggc ggcaggctgc ggcgttacgc
     3241 cgattatgtc gatgcgtcgc tggctggcga agaaccgtcc acaggccgat gtgcaggtga
     3301 tctacaacgt gcgtacgccg caggatgtga ttttcgccga tgagtggcgt aactatccgg
     3361 taacgctggt ggcggaaaat aacgttaccg aaggctttat cgctggtcgt ctcactcgcg
     3421 aactgctggc aggtgtccct gatttagcct cacgtaccgt gatgacctgt ggccctgctc
     3481 catatatgga ttgggtagag caggaagtga aagcgctcgg cgtgacgcgt ttctttaaag
     3541 agaaattctt caccccagta gcggaagcgg cgaccagcgg tctgaaattc accaaactgc
     3601 aaccggcacg agaattttac gccccggttg gcaccacgct actggaggcg ctggaaagca
     3661 ataacgttcc ggttgtcgcc gcctgccgcg caggtgtttg cggctgctgt aagacgaaag
     3721 tggtttccgg tgaatatacg gtgagcagca caatgacgct gaccgacgcc gaaatcgctg
     3781 aaggttatgt actggcctgc tcctgccatc cgcaggggga tttggttctc gcataatcgc
     3841 ctgatgcccg atgatattcc tttcatcggg ctatttaacc gttagtgcct cctttttctc
     3901 ccatcccttc cccctccgtc agatgaacta aacttgttac cgttatcaca ttcaggagat
     3961 ggagaaccat gaaacaaacg gttgcagctt atatcgccaa aacactcgaa tcggcaggcg
     4021 tgaaacgcat ctggggagtc acgggcgact ctctgaacgg tcttagtgac agtcttaatc
     4081 gcatgggcac catcgagtgg atgtccaccc ggcatgaaga agtggcggca tttgccgctg
     4141 gcgctgaagc acaacttagc ggagaactgg cggtctgtgc cggatcgtgc ggtcccggca
     4201 acctgcactt aatcaacggc ctgttcgatt gccaccgcaa tcacgttccg gtactggcga
     4261 ttgccgctca tattccctcc agcgaaattg gcagcggcta tttccaggaa acccacccac
     4321 aagagctatt ccgcgaatgt agtcactatt gcgagctggt ttccagccca gagcagatcc
     4381 cacaagtgct ggcaattgct atgcgcaaag cggtgcttaa ccgtggcgtt tccgttgttg
     4441 tgttaccggg cgacgtggcg ttaaaacctg cgccagaagg ggcaactacc cactggtatc
     4501 atgcgccaca gccggtagta acaccggaag aagaagagtt acgcaaactg gcgcagctgc
     4561 tgcgttattc cagcaatatc gccctgatgt gtggcagcgg ctgtgcgggg gcgcataaag
     4621 agttagttga gtttgccggg aaaattaaag cgcctatagt ccatgccctg cgcggtaaag
     4681 aacatgtcga atacgataat ccatatgatg ttggaatgac gggattaatc ggcttttcgt
     4741 caggtttcca taccatgatg aacgccgaca cgttagtgct gctcggtact cagtttccct
     4801 accgtgcgtt ctacccgacc gatgccaaaa ttattcagat tgatatcaac ccagccagca
     4861 tcggcgctca cagcaaggtg gatatggcac tggtcggcga tatcaagtcg actctgcgtg
     4921 cattgctgcc attggtggaa gaaaaagccg atcgcaagtt tctggataaa gcgctggaag
     4981 attaccgcga cgcccgcaaa gggctggacg atttagctaa accgagcgag aaagccattc
     5041 acccgcaata tctggcgcag caaattagtc attttgccgc cgatgacgct attttcacct
     5101 gtgacgttgg tacgccaacg gtgtgggcgg cacgttatct aaaaatgaac ggcaagcgtc
     5161 gcctgttagg ttcgtttaac cacggttcga tggctaacgc catgccgcag gcgctgggtg
     5221 cgcaggcaac cgagccggaa cgtcaggtgg tcgccatgtg cggcgatggc ggttttagca
     5281 tgttgatggg cgatttcctc tcagtagtgc agatgaaact gccggtgaaa attatcgtct
     5341 ttaataacag cgtgctgggc tttgtggcga tggagatgaa agccggaggc tacctgacag
     5401 acggtactga gctgcacgac accaactttg cccgcattgc cgaagcgtgc ggcattacgg
     5461 gtattcgtgt agaaaaagcc tctgaagtcg atgaagccct gcaacgcgcc ttctccatcg
     5521 acggtccggt gttggtggat gtggtggtcg ccaaagaaga gttagccatt ccaccgcaga
     5581 tcaaactcga acaggccaaa ggtttcagcc tgtatatgct gcgcgcaatt atcagcggac
     5641 gcggcgatga agtgatcgag ctggcgaaaa cgaactggct aaggtaaaaa gggtggcatt
     5701 tcccgtcata ataaggacat gccatgattg atttacgcag tgataccgtt acccgaccga
     5761 gccgcgccat gctcgaagcg atgatggccg ccccggttgg ggacgacgtt tacggagacg
     5821 accctaccgt taatgctctg caggactatg cagcagagct ttccggtaaa gaagccgcca
     5881 tttttctgcc taccggcact caggccaacc tggtcgctct gctcagtcac tgcgaacgcg
     5941 gcgaagagta tatcgtcggt caggccgcgc ataactatct gtttgaagcc ggtggcgcag
     6001 cagtgctggg cagtattcag ccgcaaccca tcgacgcggc tgccgacggc acgctacctc
     6061 tggataaagt ggcgatgaag atcaaacccg acgatatcca tttcgcccgc accaaattac
     6121 tcagtctgga aaacacccac aacggcaaag tgttgccgcg ggaatacctg aaagaagcat
     6181 gggaatttac tcgcgagcgc aatctggcgc tgcatgttga cggtgcgcgc atctttaatg
     6241 ccgtggtggc ttacggctgc gaactgaaag agatcacgca atattgtgat tcgttcacca
     6301 tttgcctgtc gaaaggtctt gggacgccgg tcggttcatt actcgtcggt aatcgtgatt
     6361 acattaaacg tgccattcgc tggcggaaaa tgacaggtgg cgggatgcgt cagtccggca
     6421 ttctggctgc cgccgggatg tatgccctga aaaataacgt cgcacgtttg caggaagacc
     6481 acgataacgc cgcctggatg gcggagcagt tgcgtgaagc aggcgcggat gtgatgcgtc
     6541 aggacaccaa tatgctgttt gttcgcgtcg gggaagaaaa tgctgccgcg ttaggcgaat
     6601 acatgaaagc gagaaacgtg ctgattaacg cctcgccgat tgtccgcctg gtgacccatc
     6661 ttgacgtctc gcgtgaacaa ctggcggaag ttgccgccca ctggcgtgcg ttcctggcgc
     6721 gttaaggaga gagacgtgcc gcaacgcatt ttagttctcg gtgccagtgg ctacattggt
     6781 cagcatctgg tgcgaactct cagccagcaa gggcatcaga tcctggcggc ggcacgtcat
     6841 gtcgacaggc ttgcaaagct gcaactggca aacgtcagtt gccataaagt cgatctcagc
     6901 tggccggata accttccggc cctgttgcag gatatcgata cggtctattt tctggtgcac
     6961 agcatgggcg aaggcggcga ttttatcgct caggagcgcc aggtggctct caacgtccgc
     7021 gatgcgctac gcgaagtccc tgttaagcaa ttaatcttcc tcagttcgtt gcaggccccg
     7081 gcacatgagc agtctgacca tctgcgcgcc cgtcaggcta cggcggatat tcttcgtgaa
     7141 gcgggtgtcc cggtgaccga actacgtgcc ggaataatcg ttggtgcagg ttcagcggcg
     7201 ttcgaagtca tgcgcgatat ggtctacaac ctgccggtgt taacgccgcc acgctgggtt
     7261 cgttcacgca ccacgcccat cgcgctggaa aacttgctgc actatctggt ggcgctgtta
     7321 gatcatccgg ccagcgaaca tcgcatcttc gaagccgccg gaccagaagt gctcagttat
     7381 cagcaacagt ttgaacattt tatggcggtg agcggtaagc gccgctggtt gatccccatc
     7441 cccctcccca cccgctggat ttcggtgtgg tttctcaatg tgattacttc cgtaccgccc
     7501 accaccgcca gggcgttgat tcaggggctg aaacatgatc tgctggcgga tgacaccgcg
     7561 ctacgtgcac tcatcccaca acggctgatc gctttcgatg acgcggtacg tcgcaccctg
     7621 aaagaagaag aaaagctggt caactccagc gactggggct acgacgctca ggcctttgcc
     7681 cgctggcgac cggagtacgg ttatttcgcc aaacaggcgg ggtttaccgt taaagcgtcc
     7741 gccagccttg cggctttatg gcaggtggtg aatcaaatcg gcggtaaaga gcgttatttc
     7801 tttggcaata ttttgtggca gacacgggcg ttgatggacc gcgcgatcgg tcataagctg
     7861 gcgaaaggcc gcccggagcg tgaatatttg cagaccggcg atgcggtgga tagctggaaa
     7921 gtgattgtcg ttgaaccgga aaaacaactt acgctgttat ttggcatgaa agcgccgggg
     7981 ctgggacgac tgtgttttac cctggaagat aaaggtgact atcgtactat cgatgttcgc
     8041 gcattctggc atccgcacgg tatgccgggg ctgttttact ggttattgat gatccccgcg
     8101 catctgttta tttttcgcgg aatggcaaaa cgaatcgcca gactggcaga acaaagcaca
     8161 gattaatcga caaaacctgc gttattcttt catgtttccc attgcatcat gggggaaatc
     8221 acggaagaat gcgcacgtta ttttctcaac aacagtggat tgatatgaag gtactggtta
     8281 ccggcgccac cagcggctta ggtcgaaacg cggtagagtt tttatgccag aaaggcatca
     8341 gcgtgcgagc gaccggtcgc aacgaggcaa tgggtaaatt gctggaaaaa atgggcgcag
     8401 agtttgttcc ggcggatctg accgagctgg tctcatcaca agctaaagtg atgctcgcgg
     8461 gcattgatac gctgtggcac tgctccagct ttacctcgcc ctgggggaca caacaggctt
     8521 tcgatctggc taacgttcgc gccactcgcc gcctgggtga atgggctgtc gcctggggtg
     8581 tgcgtaactt tattcatatc tcttcaccct ccctgtactt cgattatcac caccatcgcg
     8641 atattaaaga agattttcgc cctcaccgct tcgccaacga gtttgcccgt agcaaagcag
     8701 ccagcgaaga ggtgatcaat atgctttcgc aggcgaatcc acaaacgcgc tttactattc
     8761 tgcgcccaca aagtctgttc ggaccgcacg ataaagtctt tattccccgt ctggcgcata
     8821 tgatgcacca ctacggcagc attctgttac cgcatggcgg cagtgcgctg gtcgatatga
     8881 cctactatga aaatgccgtg cacgccatgt ggctggcaag ccaggaagcc tgcgataagc
     8941 taccttccgg gcgtgtgtac aacatcacca acggcgagca tcgcacactg cgcagcatcg
     9001 tgcagaagct gatcgacgag ttgaatattg actgtcgtat tcgttccgtc ccctacccga
     9061 tgctggatat gatcgcccgc agcatggagc gtttaggccg caagtcagca aaagagccgc
     9121 cgctgaccca ctacggcgtc tccaagttga attttgactt tacgctggat attacgcgcg
     9181 cgcaggaaga gttaggttat cagccggtca tcaccctgga tgaaggtatc gagaaaactg
     9241 ccgcctggct acgcgaccac ggaaaactgc cgcgctaatc ctgcccgtat ttctccagca
     9301 acgcttcggc aatcgcctga gtttctgcat ccgcttcgcc gttgtataac gtcgggcgga
     9361 aatgcatctg gaacgccata atcacgcgcc gctgctcgcg cggtgtcata tcaggtttaa
     9421 cgtcataacc gtagcgcgcc aaaagctcca gtaatgatgc ggtatctacc agtgtgtgcg
     9481 gcgcgcgccc ggcaaggtaa aagttcaccc gctgcgcatc cggccaggca ccaatcccct
     9541 gctgcgccag ttgttgccag gggaataacg gccccggatc gtctttgcgc tgcggcgcaa
     9601 tatccgcatg cgccactacg ttttccggct tgatgtgata acgggcaata atatctttcg
     9661 ccagcggaat aagcgcctga atctgtgcct gttcaaacgg ggcaaaatat ttcactccgg
     9721 atgatttttg ccagccacgg ttttccagct caatgccaat agaggtgtcg ttaagacgcg
     9781 ttgccccgcg ccaggcgcta atccccgcat gccaggccag ttcctgttcc ggcaccagtt
     9841 gccagatgcg cggtttaccg ttgtatcgcg gtggtaccgc agggaccaga taatgcgagc
     9901 tgacctgctt atcggtcagt gtcgccagcg agctatcaaa atcatctgcg gtgtagtgga
     9961 taaccagcac tttaatgcgc ggatacgccg cctgcgcctg gcgtcgggta tcaagctgat
    10021 atccctcttt ctcgacaatg cctttttcgc ctgcacaccc tgccagtaac agagcgacag
    10081 cgaccagcca aaagactctt ctcatcgacg cgttttcacc gccgtaccgc tgacgctaac
    10141 catcagcata ctgccatttt gcccgaccgt ttcgtagtcg atatcaatac cgactacggc
    10201 atcagccccc agcgcccggg cctgatcgcc gagctcctca aaggcgatct cccgcgcttt
    10261 acgcaattct ttttcataag ccccggagcg tccgccaacg atatcgcgga taccggcaaa
    10321 gaaatcacgg aaaatattgg cacctaaaat cgcttcgccg gtcaccacac cgcaatattc
    10381 gacgatggtc tggccttcca gagttggggt tgtagaaaat tgcatgatcg atctccttgt
    10441 ttgctaagcc ttcgatcttc aaagcattat cggactgata cgctattatt gaaaggatat
    10501 cattattatc gtagaacgct ttcaaggtgt tcgcttataa ggaaatcatc atgcgctata
    10561 gcaaattgac catgcttatc ccttgcgcac tgctactcag tgcctgtacc accgtcacgc
    10621 cagcttataa agataacggc ccccgcaccg gttcctgtgt ccaggggggg cctgatagcg
    10681 tggctcaaca gttctatgac taccgcattc aacatcgcgg caatgacatt accgctctgc
    10741 gcccctattt gagtgacaaa ctggcaaccc tacttagcga tgccagccgc gataatagtc
    10801 atcgtgaatt gttaagcagc gatccgttct ccagtcgaac caccttgcca gacagcgccc
    10861 acgttgccag tgcatcgact atccctaatc gtgatgcccg taacattcca ttgcgcgtgg
    10921 atctcaagca gggtgaccag ggctggcagg atgaagtgtt aatgattcag gaaggccagt
    10981 gttgggtcat cgacgatgtg cgctacttag gcggtagcgt tcatgccaca gcgggtacgc
    11041 tccgtcagtc catcgagaat cgctaacgtc ttgatcaacc aataaacccc gtaaattgta
    11101 cggcatttcg tgaggaatgc cgacatttat gctcgccgac caccgccccc gttattttgt
    11161 gctatgttta ttgaataatg cgctttgctt ttaactttta aagcagaaat attgcataat
    11221 tattctgtca aaggtactat cttcgacctc aatagctatc agactgccag tatacgagtg
    11281 tcaatgagta ttcaattaaa cggcattaat tgcttctacg gcgcgcatca ggcgctgttc
    11341 gatatcacgc tggattgccc acagggcgaa acgctggtgt tacttggccc cagcggcgcg
    11401 ggtaaaagct cgctgctgcg tgtactcaat ctgcttgaga tgccgcgctc cggtacgctc
    11461 aacattgcag gcaaccattt cgatttcacc aaaaccccct ctgacaaagc gattcgcgat
    11521 ttgcgtcgta acgttggcat ggtgtttcag caatacaacc tgtggccgca tctgaccgtg
    11581 cagcaaaacc tgattgaagc gccctgtcgt gtactggggt tgagtaaaga tcaggcgctg
    11641 gcccgtgcag aaaaactgct ggaacgtctg cgtctcaaac cttatagcga tcgttacccg
    11701 ctgcatcttt ctggaggtca gcagcagcgt gttgctattg cccgtgcgtt gatgatggaa
    11761 ccgcaggtac tgctgttcga tgaaccgacc gccgcactgg accccgaaat tacggcacaa
    11821 atcgtcagca tcattcgtga gctggcagaa acgaatatta cccaggtgat cgtcacccac
    11881 gaagttgaag tggcgcgtaa aaccgccagc cgagtggtgt atatggaaaa cggtcatatc
    11941 gtagaacaag gcgacgcgag ctgctttacc gagccgcaaa ccgaagcatt taaaaactat
    12001 ctctctcact aagattcggg ataacaacaa tgaaaaaagt tctgattgcc gcgttaattg
    12061 caggttttag tctttccgcc acagcagccg aaaccattcg ttttgccacc gaagcctcct
    12121 atcctccgtt tgaatcgatt gatgcaaaca accagatcgt tggttttgat gtcgacctgg
    12181 cgcaggcatt gtgtaaagag attgatgcaa cctgcacttt ctctaaccag gcgtttgaca
    12241 gcctgatccc aagcctgaaa ttccgtcgcg tagaagcagt gatggcgggc atggatatca
    12301 ctccggagcg tgaaaagcag gtgctgttta ccaccccgta ctatgacaac tctgccctgt
    12361 ttgtgggtca gcaaggcaaa tacaccagtg ttgatcagct gaaaggcaaa aaagtcggcg
    12421 ttcagaacgg gacgacacac cagaagttca ttatggataa gcatccggaa atcactaccg
    12481 tgccatatga cagttaccag aacgcaaaac tggatctgca aaacggtcgt atcgacagcg
    12541 tatttggtga caccgcagtg gtaaccgaat ggctgaaaga taacccgaaa ctggcggcag
    12601 tgggcgataa agtgaccgat aaagattact tcggtactgg cctcggcatc gcagtacgcc
    12661 agggcaacac tgaactgcag cagaaactca acactgcgct ggaaaaagtg aagaaagatg
    12721 gcacttacga aaccatctac aacaaatggt tccagaagta attcctgatg aatgaatttt
    12781 ttcctttagc aagcgccgcc gggatgaccg tcggccttgc cgtttgtgca ttgattgtcg
    12841 ggctggcgct ggcgatgttc tttgcggtat gggagtctgc aaaatggcgt cctgtcgcgt
    12901 gggcaggttc agcgttggtg acggttctgc gcggcctgcc agaaattctg gtggtgctgt
    12961 ttatctattt tggctcctcg cagctgctgc tgacgctttc ggatggcttc actatcaatc
    13021 ttgggttcgt gcagatcccg gtgcagatgg acattgagaa cttcgacgtt agcccgttcc
    13081 tttgtggtgt catcgctctg tcactgctgt atgccgccta tgcctcgcaa acgctgcgcg
    13141 gcgcgttgaa agcggtgccg gtgggccagt gggaatctgg tcaggcgctg gggctgtcga
    13201 aatcggctat ctttttccgt ctggtgatgc cgcagatgtg gcgtcatgcg ctgccgggtc
    13261 tcggtaacca gtggctggtg ctgctgaaag ataccgcgct ggtcagtttg attagtgtga
    13321 atgatttaat gctacaaacc aaaagcatcg ctacccgtac ccaggaaccg tttacctggt
    13381 acattgtggc ggcggcgatt tacctggtga tcaccctgct cagtcagtac attctcaaac
    13441 gcattgacct gcgcgcgaca cgttttgagc ggaggcccag ctaatgtttg agtatttacc
    13501 cgaactgatg aaagggttgc acaccagcct gacgctaacc gttgcctcgc tgattgtggc
    13561 actgattctg gcattgattt ttaccatcat cctgacgctg aaaacgccgg tgctggtgtg
    13621 gctggtgcgg ggctatatca cgctgtttac cggtacgccg ctgctggtgc agatcttcct
    13681 gatttattac ggaccgggcc agttcccgac gttgcaggag tatccggcac tgtggcattt
    13741 gttgtcagaa ccgtggctgt gtgcgctgat tgcgttgtcg ctgaacagtg cggcgtatac
    13801 cacgcagctg ttttacggtg caattcgtgc gatcccggaa ggtcagtggc agtcctgtag
    13861 cgccctggga atgagcaaaa aagatacgct ggcgatcctg ctgccgtatg cctttaaacg
    13921 ctcgctctct tcttattcca acgaagtagt gctggtgttc aaaagtacct cgctggccta
    13981 caccattacg ctgatggaag tgatgggata cagccagttg ttgtacggac gcacctacga
    14041 tgtcatggtg ttcggcgcgg cagggattat ttatctggtc gttaacggcc tgctgacgct
    14101 gatgatgcgt ctgatcgagc gtaaagcgct ggcgtttgag cgaaggaatt aatttggctg
    14161 cgtgctgatg ccggatgcgg cgcggacgcc ttatccggcc tacatagtgc ataaattctt
    14221 ataatgaaga cggacaactc actaagttgt ccgtcttttt ttatttcatt taaattattt
    14281 aatcatattt attgcatata aattcacttg atggcattgt tatcccatgc cgcagacacg
    14341 gccaaaaatc ataagattga cagacgggag ttccatcatg aaaaagttag ttcttgccgc
    14401 attacttgct tcctttactt tcggtgcttc tgccgcagag aaaatcaatt ttggcgtttc
    14461 agccacctat ccaccctttg aatctatagg tgctaataat gagattgtcg gctttgatat
    14521 cgacctggca aaagccttgt gcaaacaaat gcaggcagaa tgtactttta ctaatcacgc
    14581 gttcgacagc ctgatcccgt cactgaaatt cagaaaatat gacgccgtaa tctccggtat
    14641 ggatatcact ccagagcgta gcaaacaggt atcgtttacc acgccctact atgaaaactc
    14701 agccgtcgtg attgccaaaa aagataccta caaaacgttt gccgatctga aaggcaaacg
    14761 tattgggatg gaaaacggta ctacgcacca gaaatatatt caggatcagc acccggaagt
    14821 gaaaactgtc tcctatgaca gttatcagaa tgcctttatc gatctgaaaa atggccgtat
    14881 tgatggggta tttggtgaca cagcggtggt taatgaatgg ctgaaaacca acccgcaact
    14941 gggcgttgcg actgaaaaag tgaccgatcc gcaatacttc ggcaccggcc tgggtatcgc
    15001 tgtacgtccg gataacaaag ctctgctgga aaaactgaat aacgcgctgg cagcaattaa
    15061 agctgacggt acatatcaga aaatcaatga ccagtggttc ccgcagtaag attcagaaag
    15121 ccgcacctgc ggctttttta ttgcttaacc agcagtgtca gcacttcgta atgcgcagtg
    15181 tgcgggaaca tatcaaaaag ctgtacccgt tcgatgcagt aacctggcag ttcgcggata
    15241 tctttcgcca tggtttgggc gttacagctg gagtagataa taaaacgcgg tgccatcgtt
    15301 gagagataat cacacagcgg tttaccaatg ccgcggcgcg gcgggttaac cagcaccagc
    15361 tccggcacct ccccctgagc agtggcaaac tgagtggagt ccagcgcctg aaattgcaaa
    15421 cgcgttaagc ccagttcagc ggctgactgc tttgcacagg caatggcctc tggcgcaatt
    15481 tcgatcccgg ttaactgcat gtcaggcatc gcgcagtgta aaccaaagcc ccccacaccg
    15541 cagaacagat cccacatatg tttaaccgac agctgtcgta cccagtcgcg cgcggtggcg
    15601 tacaactggc tggcgaccgc cggattagtc tggaagaaac tttgcggacg gatccacagc
    15661 ggtacgtcat taaaacgctc tgccagcgcc tgttgttcag tcaggtaaat ctccgtctcc
    15721 ccttccataa tcgccatatg taccggctga atattgacgg taataacttt cagctgcggt
    15781 agttgttcct gtaaccacgg cagcgcctta cgcagttgcg ccagcttggt atcagaacgc
    15841 agtacaaagc gcagcatcat gccgccatcg ctctggcttt cagtcagcag aatgtatttc
    15901 agttcgccac gcttacgcgc cacgttgtag ggcgttaacc ccgctcgggc gataaacggt
    15961 tttagcgccg caaaaacggg cgcaaatgag gcaggataaa gcggacagtc acaaaggtct
    16021 tccggcgtgc cgtcgcgatg cagcataccg agcagtggtt tttcaacgct accactcacc
    16081 accattttgg ctttattacg aaacccttgt tccgggcctg acaccggcgc gcaccattcc
    16141 tcaaccggaa agtcggcgag cagattttta agatcggcgg ttttagcgga gagttgctct
    16201 ggaatcggct gcgttatcca ctgacaggaa cgacagcgac ccgcgtcgta aagtgcgcac
    16261 tgcatacatt gaccttcaca tcatcagggg cgacgattat acacattatt gcaactggaa
    16321 gaaccgccga ctggtcgagg gaacgaacag cagcatcagg atgagcatat ccggcagctt
    16381 ctgcagcatc aggctatgga agatttcacg ttttgattca ccgggaatgc tgaacagctc
    16441 cggataacca taccccagcg aagctgccca caagtaactt gcggccgtga tttgtgtcaa
    16501 cagatacagc cagcgcgccc agcgacgccc tttcaccagt gaaaacgcac accagatctc
    16561 aatgaaaacc agcaccagac tgctcaaaaa gaccagcgtt aaactccagg tttgtacgct
    16621 gcgatgaatg aactcgccaa taccgcgcac gcccagcgta ttgaaaatca tcagcacgtc
    16681 gaggccacgg atcataataa tggcgagcgc cgccacctgc accagcgcag gcacattcag
    16741 gcgagcatga gatgaagatg ttttcttaaa aaatcccaac gtttcgtctt ccatgaaaac
    16801 aatgccgcga catgcgcggc attatgtagc caggttggca aattttagtg tcttcagcca
    16861 cgtcttgcac gctggatgtc gcgtatccgc tgtttttctg cgcgcgccat cagataccag
    16921 gcgataaatc cgacaattcc gaccgcgccg aggatcaatg ttgccagggc gttgatttcc
    16981 ggattcaccc ccatccgcac gctggaaaag accagcatcg gtaaagtggt ggctcccggc
    17041 ccggaaacaa agctggcgat caccagatca tcaagcgaca aagtaaaagc cagtaaccag
    17101 ccagaaatga tcgcgggcat gatcatcggt agcgtaatga caaaaaacac tttcagcggc
    17161 gtcgcaccga gatccatcgc ggcttcttct atcgagcgat ccagttcccg cagacgcgac
    17221 gaaataacga ctgccacata agccgtacag aaagtgacat gcgccagcca gatggtgagc
    17281 ataccgcggt ccgcaggcca gccaatagca tgagcaagcg cgacgaataa caacaatagc
    17341 gacaagcccg tgatgacatc tggcatcacc agcggcgcgg tgatcataaa ggcaaaacca
    17401 tttgatccgc gaaacctacc aaagcgcacc agcacgaccg ccgcaatcgt cccgaggatc
    17461 gccgccgccg tcgccgcaca ggccgcaatt gtcaggctta aaccaaccgc actcatcatc
    17521 gcgttatcgc gcaataactc accataccag cgtgttgacc agccggccca caccgtcacc
    17581 agtttcgagc tgttgaatga atagatcacc agcatcagca ttggcgcgta gagaaaggtg
    17641 aagcccagca gcaaaatcac aatccgccag ggcgaacgaa caaccggtaa attattcatc
    17701 cgtgttctcc cacgcttttt tgctggtgtt tgtgaaacca cattatcggc acaattagca
    17761 gcagcaacat gatgatcgct accgccgagg ccaccggcca gtcgcggtta ttgaaaaact
    17821 cctgccacag cacgcgcccg atcatgatgc tgtccgggcc accgagcagt tcagggatca
    17881 caaactcgcc cacagccggg ataaacacca gcatcgatcc ggcaataatc ccgcctttgg
    17941 tcagcggcac gatcacagta aagaacgttt tcagcggtcg tgcaccgaga tccagtgccg
    18001 cttccaccag cgaataatca atacgaatca aggcggtgta gatcggcagc accataaacg
    18061 gcacataggc gtaaacaatg ccgatataaa ccgccaggtt ggtatgcaga atggtcaacg
    18121 gttgatcgat aacccccagc cacagcagaa aattattcag cacaccgttg ttttttaata
    18181 ttcccatcca ggcataaacg cggatcagaa acgaggtcca cgacggcagg atcaccagta
    18241 gtaataagat attacgggtc gaaggcttac tgtgcgccac cgcccacgcc agcggatagc
    18301 cgatcagtaa acagcaaaat gtcgaaatcg cagccacctg tagcgactgg agataagcat
    18361 cgaaatagag cggatcgtca gtcagttgca gaaaattacc gagattaaga gtgatggaca
    18421 gttgcccgtc agcccactcc atcagttcgg tataaggcgg aatggcgcgc gccatctccg
    18481 ccagacttat tttaaagacg atcagaaatg gcagcagaaa cagcaggatc aaccagatat
    18541 acggcaacgc aatgaccagt ttgcgcccat gcttcatttg cagctgcgac aaccacagct
    18601 taaatccgcc cggcggtttg ggctgggcag caggttcaag tgtactcatc tttgctcctt
    18661 aaaccgtcag caccacacag ctgtccactt cccagcacaa acgcacttcg tcgccccagg
    18721 ttggtaaccc tttgcgatat cgatgagcgt tttgtagctg agcgctgatc atctgtccgc
    18781 tcttgagacg aacgtgatac accgaaagat cgccgagata ggcaatgtgc atcacctcac
    18841 ctaccgcaaa gttaaaaccg tcagcgggag gttcctcgca aagcatgatt ttttccgggc
    18901 gcagcgccac atgcaccggc acattatcga ccaccgaagc atctacatcg actttcagtg
    18961 gatgcaccag ccccggcgaa taaagcacca gaccatcttc ctgacgctct ttgagtacac
    19021 cttcaaagac attaaccgag ccaataaatt cggcgctgta gcgagtggtc ggatgctcgt
    19081 agatctcttc cggttcgcca atctggacaa atttccctcg attcataatg gcgatacgcc
    19141 ccgccatggt catcgcctct tcctgatcgt gggtgaccat cacacaagtc acaccgacac
    19201 gctccagaat atccaccact tcaagctgca tccggtcacg cagcttttta tccagcgcgc
    19261 ccataggctc atcgagcagt aatagtttcg ggcgcttggc aaggcttcgg gccagtgcca
    19321 cacgctgtcg ctgaccgccg gaaagctgat gcggtttgcg tttggcgaac tcctgcatat
    19381 gcaccagccc gagcatctca ttgacccggc tggcaatttc cgctttcggt agtttgtcct
    19441 gtttcaggcc aaaagcgatg ttctgttcca cggtcatatg cggaaacagc gcgtaagact
    19501 gaaacatcat atttatgggg cgcaggtaag gcggaacctg tgacaaatcg acgccatcaa
    19561 gcattatctg tccggcagaa ggttgttcga aacctgccag catacgcagc agcgtggact
    19621 tgccacagcc ggatgcgccc agcagcgcga agatttcgcc tttatagatg gtcaggctga
    19681 catcatcgac cgcatgctga ccatcgtaag atttagtcag attacggatt tctaatagcg
    19741 gcgtcagcac cttacgggtt ttcgcctgcg ggcgagggat agcgtcattc acggctgctc
    19801 tccggcattg aatgtgtgca agcgcaccat aatggtgcga attgccggcg ggcgtggtgc
    19861 gcccgtccgg catctacgac tgcggattat tttccgctct tcactttggt ccacgcgcgg
    19921 gtgcgcacac ggtcgatttt cggatcctgt actttcagag tgaacagctt cgcacgaaca
    19981 tccgcaggcg ggtaaatacc cgggttatca cggacttccg cactcaccag cggcgtagct
    20041 gctttattgg cgttagcata gaacacatgg tcggaaatat gcgccaccac atccgggcgc
    20101 agcaggtaat tcaggaactg ataggcttcg tctttatttt tggcatccgc aggcatggcg
    20161 aagacatcaa agaacgccat cgccccttct ttcggaatcg agaacgagac attaacgcca
    20221 ttcttcgctt ccttcgcgcg gtttgacgcc tgccagacat cgcccgccca gccaatagcg
    20281 acgcagatat cgccgtttgc caggtcgtta atgtattgag atgaatggaa ataacgaatg
    20341 ttcgggcgca gctttaacag cagatcggtt gccggtccgg tgtaatcatc tgctttagtg
    20401 ctgttgggat ctttaccgag ataattcagc acggtagcaa acacttcttc cggtgcatca
    20461 aggaaagaga cgccgcagct tttcagtttt tccagatttt caggcttgag gatcaagtcc
    20521 cagctatcga cgggcgcgtt ttcgcccagc accgctttaa ctttatcaac gttatagcca
    20581 atcccggtcg tcgcccacat atagggcata gcaaatttat tgtcgggatc gtgtttggca
    20641 actaacttca gcagctccgg atcgagattc ttccactccg gcaatttgct tttgtccagc
    20701 ggctggaaaa ctcccgcagt caactggcgt tcaagaaagc ttgctgacgg caccaccagg
    20761 tcaaagccgg tgctacccgc cattaatttg ccttccagta cttcgttaga gtcgaaaaca
    20821 tcgtagacga ctttaatacc ggtttctttt tcaaaattgg ccaccgtgtc cggggcgata
    20881 taatcagacc agttataaat gtgaagtgtt ttttgttcag ccgcgagcgt gccgacagag
    20941 acggccatca gagcacccgt aaccagaccc gatagccatt ttttatttaa ggcggtcata
    21001 tctctttcct tctgaaagtt cgttaacaaa cgaacaaaag ttgtcacact gaaagatatg
    21061 caataaacgt gcatattttt tatcggcgcg gtgtaagaaa agagaactct ctcccggcca
    21121 gcactgctcg ctatataaaa gcatcgcaag aataacttta gccagggttt gagactatag
    21181 ctcggattaa aaaacggtat ttaacaaatt tttatgcgta tttgctctat gtgataaagc
    21241 caaatcggca caagagaagt gaaataatct ttaagaaaag gttaagaata gcagataatt
    21301 ttgcagcacg cagtcactgc ggcagcgact gcgtactcga aaggagttat cagtggagga
    21361 aatagttttc ggtaacgttg ttttgccctt catcgtcagt cggtaacagc agttgatggg
    21421 cattagcttc aagaatgacc atcgaaatct gctcttcgct ctgacgcacg aaccatgcaa
    21481 actgctcata ggttacgccc tgcataacgg ataaagactg gcaaaccacc agctttggca
    21541 gattatcatc ctgcatatca agaaatgctt tcacggtcag cgaactggca ttgatggcag
    21601 ataaatccgc cgccagcggc aatacggctg aaggtcggac ttccgccatg gcagaaaaca
    21661 ggatcgtgtt atcgatcaga tcgattttgg catcaaagac accgtcgaaa ttctgcatat
    21721 ggggcagatg cagagcctga cagttatcac attcaaaaaa actcatcccc aggtcatcga
    21781 gccattgacg cagcgtatcc agaccaggaa cgaccagcga tgtcataatt ttgtacaacc
    21841 tctttcgata aaaagaccgg cacagcttac gcaaaaagcg caggcaaaac catgatcagt
    21901 aatgtgattg cgactaacca cccgttttca ggcaatattc cgtcgtagcg tggcgttcga
    21961 tccagcggat cattttaccc gcgatgtcga taccggtggt tttttctatt ccttccagcc
    22021 ccggcgacgc attcacctcc atcaccaatg gcccgcgatt agcacgcaga atatccacac
    22081 cagcaacgtc cagcgccatc gttcgcgcgg cttttatcgc gatttcacgc tcctgtggtg
    22141 tgatactggc gacgcttgcc gcgccgccac gatgcaaatt ggaacgaaaa tcgccctctt
    22201 tcgcccgccg ttcaatcgca gcgaccactt catcgccaac aaccagacag cggatatcgc
    22261 acccttgcgc ctctttgata tattcctgca ccagaatatg cgcgttcaga ccgcggaaag
    22321 cgtcaatcac gctttccgcc gcctggcgcg tctccgccag cacgacacca attccctgcg
    22381 tgccttcaac cagcttgacc accagcggcg caccaccgac catgtcgatt aaatcgctgg
    22441 tatcatccgg cgaatgcgca atgcccgtga caggcaggtc gatgccctga cgcgccagca
    22501 gttgcatgga acgcaatttg tcacgcgccc gggcaatggc gaccgactca ttgagcggat
    22561 agctccccag catctcgaac tggcgcagtg ccgccgtccc ataaaaggta atggcggtac
    22621 caatacgcgg gatcacagca tcaaaatggg gtaacttgcg gcctttgtag tgaatagaag
    22681 acgccgcagg atttatgttc atgtagcaag aaagcggatc aagaatttca accaggtgac
    22741 cgcgctgtat agcggcttca cgcagccgct tacacgaata gagcgttcca tcccgggaca
    22801 atatggcaat tttcaccctg cacctctctg tcagacctgg tcaaaagccc gcgtatcata
    22861 catcggcgtg gcggttttag cgcgtcgccc aaccctgttt gtgcaaataa tccagtataa
    22921 atgggcggct ttctttaatg attgttcggc ggatatgatc gctccaggta tcccggcgat
    22981 tattgctgcc acgggtgagg taatattccg ccagttgttc gtcatactgc gccagtgcgt
    23041 ctttatccag cggttgatag ttgttttcat gcaccaaaat ggaggccggt aaacgcggct
    23101 taagatccgg attatccgca ggccagccaa ggcacagccc aaacagcggc agaacatgct
    23161 gcggtaattt cagcagctcc gtcaccgctt caatattatt gcgcaggccg ccgatatata
    23221 ccccgcccaa tcccagcgat tccgctgcgg ttaatgcatt ctgcgccatc attgccgtat
    23281 caacgacacc gagcaacagt tgttccgcca ggccaagttg tgcatccgga cagatctgta
    23341 aatggcggtt aaagtcggca cagaacaccc agaactccgc cgcttgcgct acgtgttttt
    23401 gcccgccggt cagcgtcacc agttcatcac gtaacgcttt gtcggtaatg cgaataatgc
    23461 tactgcactg caaaaaactg gaactggacg tcgcacgggc gctgttaata atcgcctcac
    23521 gctgcgcttc ggaaatgggt tcatcagtga aatggcgaat ggagcgatgg ccacaaataa
    23581 gttcaatggt tggcgtcatt atctttttct ctttctgaac gtgaatattg cggtggacgg
    23641 ttcatcagct gtggggcaag acgttttgcc acctgaagaa taaccaccac cgcagcggga
    23701 agcatgagca aaacaccgag aaaaatcatc agaatctgca cttctggccg agaaaatggc
    23761 tcaggcagcg acagggagcc gcttaccgac agcagcgcca ccgccagcaa catcattccg
    23821 ataaattcca gtatcaacac gcttttaggc aatttaccga tcgcgcgcat acgcttccct
    23881 ctgcaaagtg agccttcagt ctaaaacttt tcactgtatt gtgtttaaca gttatagctt
    23941 ttagcaatta atgcaacagg ttaaacctac tttcagcgaa tacattttag cgtgatcatt
    24001 acaggtataa atctatgagg agagaaataa tgcaaaccgt tatttttggt cgtccgggtt
    24061 gcccttactg tgtgcgtgca aaagatctgg ctgagaaatt gagcaatgaa cgcgatgatt
    24121 tccagtatca gtatgtagat attcgtgcgg aagggatcac taaagaagat ctacaacaaa
    24181 aggcaggaaa acccgtagaa accgtgccgc agatttttgt cgatcagcaa catatcggcg
    24241 gctataccga ttttgctgca tgggtgaaag agaatctgga cgcctgatcg tctgacaagc
    24301 cctcgcgttg agggctttat tgattttttc tgtgctgtgg tttaaacaaa ctactgataa
    24361 ataagaaaca caatgccccc agcgcacacc agaacaccgc acttaataac catgccagct
    24421 cttgccagaa tgagcgcgtc ggtgaaaaaa acagccgcat aatgagcatc gaacagggtg
    24481 ccgccagcat tgcgccaaac agaggtttca ggacttctct acgatgtgaa aagaagctgg
    24541 caactgcacc aggaagaatg aaaaatagca aaccgatttc aggatgcccg gcagcccgaa
    24601 aagcgccttt catgtgcgtc gccagaaaaa gacacaccac aatgaagagg acaaaacagc
    24661 agattgcccc cgcccaacgt tgtttatgtt tcactcgttc ctcctgacac tgcgtctatc
    24721 gaacacattt ttcgccagtg tggcgttcag taagataaag ccgcttcgca ttccatgcta
    24781 atataggcca acgcaattca tatagccgtt gatacctaat gtgattacac tagtaaaata
    24841 tattgttact ttactatcgt ttaggtgcgc tgaatgaatc tgcgccctga attctggtaa
    24901 aaaacattat cgtaaattac catttctttc aacagcttac tagtaaacaa gaagttagcc
    24961 tccgtgaata taaacgtcgc cgaattgtta aatgggaatt acattctgtt attatttgtg
    25021 gtcctcgcgc ttgggctatg tctcgggaaa ttacgacttg gttcgatcca actgggtaat
    25081 tccattggcg ttttagtcgt atcgctgtta ttaggccaac aacatttcag cattaacacc
    25141 gacgcgctta atcttggctt tatgctgttt attttctgcg tcggggtcga agccggaccg
    25201 aacttttttt ccattttttt tcgcgatggg aaaaattacc taatgttagc actggtgatg
    25261 gttggcagtg cgctggtcat cgccttaggg ttaggtaagc tgtttggctg ggatattggc
    25321 ctgacggccg gtatgttagc aggctctatg acgtcaacac cggttctggt cggtgctggc
    25381 gatacactgc gtcattccgg catggaaagc aggcagctct cattggcact ggataatctg
    25441 agcctcgggt atgccttaac ctatttaatc ggcctggtga gtttgattgt tggtgcgcgt
    25501 tacttgccga aattgcagca tcaggactta cagaccagcg cccagcaaat cgcccgcgaa
    25561 cgtggcctgg acacagatgc caaccgtaag gtttatttgc cggtgatccg cgcctaccgc
    25621 gtcggcccgg agctggtagc ctggaccgac ggcaaaaatc tgcgtgaact gggtatttat
    25681 cgacaaactg gctgttacat tgaacgtatt cggcgtaacg ggattctggc aaatccagac
    25741 ggtgatgccg tactacaaat gggcgatgaa atagcgttgg taggctatcc tgacgcccac
    25801 gcccgactcg atcccagttt ccgtaatggc aaagaagttt tcgatcgtga ccttctcgac
    25861 atgcgtatcg tcactgaaga agtggtcgtt aaaaaccata acgctgttgg caaacgcctg
    25921 gcacaactga agttgaccga tcacggttgc ttccttaacc gcgtcatacg tagccagatt
    25981 gagatgccga ttgatgacaa cgtcgtgctt aacaaaggtg acgttttaca agtcagcggc
    26041 gatgcccgcc gggtaaaaac catcgccgat cgcatcggct ttatctcgat tcacagccag
    26101 gtcactgact tgctggcatt ctgcgccttc tttgttattg ggctgatgat cgggatgatc
    26161 accttccagt tcagcacatt cagtttcggt atggggaacg ctgccgggtt gttattcgcc
    26221 ggaattatgc tgggctttat gcgtgctaac cacccgacct tcggttacat tccgcagggt
    26281 gcattaagca tggtgaagga gttcggcttg atggtgttta tggcaggcgt tggtctgagc
    26341 gctggtagcg gtattaataa cggcctgggc gcgattggcg gtcagatgtt gattgccgga
    26401 ttgattgtca gtctggtgcc agtggttatc tgcttcttgt tcggtgctta tgtattgcga
    26461 atgaaccgcg cactgttgtt cggcgcaatg atgggcgcac gcacctgcgc gccggcaatg
    26521 gagatcatca gtgatacagc tcgcagtaat atcccggcac tgggctatgc gggcacttac
    26581 gcaatcgcca acgtcctgct gacgctggca gggacaatca tcgtcatggt atggccagga
    26641 ttaggataaa actgaagttg ccctgaaaat gaaatttttt ttgcacaacc gcagaacttt
    26701 tccgcagggc atcagtctta attagtgcca ctgcttttct ttgatgtccc cattttgtgg
    26761 agcccatcaa ccccgccatt tcggttcaag gttgatgggt tttttgttgt ctaaaattta
    26821 tctactaccc tgcaaccctc tcaaccatcc tcaaaatctc ctcgcgcgat agcggcttac
    26881 ggtcggtgac aaaatgcagc gtcatcccct caataaacgc atcaagcgcg cgggcggttc
    26941 cgggttcaaa ccattgttcg agcgtttgct gactgcgctg catccagttt tgcattaccg
    27001 tttttaatag cggttttcgg ctagccagcg cgtagagctg gtacatcagc tccatgttat
    27061 ccggcgttgc aacctgtgag ctgtaaatca tatcggtgat agcctggcat gcgcccggag
    27121 catcactaac atcgctaaaa aatgcctgat attgccggga catgatctca gtaaaactgc
    27181 tgaacgcctc cagcaacaac tcatcaattc ctgaaaaata gtaggtcatc gaccccaacg
    27241 gtaccccggc aagggtagca attttgcggt gcgtaacagc atgtattcca taaagtttca
    27301 ccgcctccag cgtggcctgg ataatttttt ctcgccgttg cggatcgtta gcgcgacgca
    27361 tgtcatttcc tctctctgca attgtgtaca aatgtacaca accttgctaa tgttgtcttc
    27421 cctcttctta ttttgacgtg atctatgacc gtaaattctt cacgtaatgc attgaaacgc
    27481 cgaacctggg cgctgtttat gttcttcttt ttgccaggcc tgttaatggc gtcctgggca
    27541 acccgtacgc ctgctatccg cgatattctt tctgtttcga tcgctgaaat gggcggagtc
    27601 ctctttggtc tgtcgatcgg ttcaatgagc ggtattctct gctcggcgtg gttagtgaaa
    27661 cgctttggga cgcgtaatgt catcctggtc acgatgtcct gcgcattgat cgggatgatg
    27721 atactaagtc tggcactctg gctgacatcg cccctactct tcgcggttgg tctcggcgtc
    27781 tttggggcaa gttttggttc tgcggaagtg gcgataaacg ttgaaggtgc tgccgttgag
    27841 cgagaaatga ataagacggt tttgccgatg atgcacggtt tttatagcct gggcacgctg
    27901 gcaggcgctg gtgtcgggat ggcactaacg gcctttggcg ttccggcaac ggtgcacatt
    27961 ttattggcgg cgctggtagg catcgcgcct atttatatcg ccattcaggc aatccctgat
    28021 ggtacgggca aaaatgctgc cgatggcacc cagcatggtg aaaaaggcgt acctttttat
    28081 cgcgatatcc agttgctgct gattggtgtt gtggtgctgg cgatggcctt tgccgaaggt
    28141 tctgccaacg actggttacc cttattaatg gttgacggtc acggttttag ccctacttcc
    28201 ggctcgctga tttatgccgg ttttaccctg gggatgaccg ttggacgctt taccggcggt
    28261 tggttcattg accgttacag tcgcgttgcc gtggttcggg ccagtgcact aatgggggcg
    28321 ttgggtattg ggctgattat ttttgtcgat agcgcctggg ttgctggggt gtctgttgta
    28381 ctttggggac tgggtgcctc gttgggcttc ccgctgacca tttctgccgc cagcgatacc
    28441 ggccccgatg caccaacccg cgtcagcgtg gtagctacga ccggttatct ggctttcctc
    28501 gtcgggccgc cactgctggg ctatctcggc gaacattatg gattacgtag tgcaatgctg
    28561 gttgtactgg cgctggttat tctcgcggct attgtcgcga aagccgtcgc caaacccgat
    28621 accaaaacgc agacggcgat ggagaatagt tgatgagcat taaattaatt gcggtagaca
    28681 tggatggtac tttcttaagc gatcaaaaaa cctataaccg tgagcggttt atggctcagt
    28741 atcagcaaat gaaagcacaa ggcattcgct ttgtggtcgc cagcgggaat caatattatc
    28801 agttgatctc tttctttccg gaaattgcta atgaaatagc ctttgtggct gaaaacggcg
    28861 gctgggtagt gagcgaaggc aaagatgttt ttaatggcga gctgtcgaag gatgcgtttt
    28921 ctactgtcgt ggaacatttg ctgacgcgcc cggaagtgga aattattgcc tgcggaaaaa
    28981 atagcgccta tacactcaaa aaatatgacg atgccatgaa aacggtggcg gaaatgtatt
    29041 atcaccgtct ggaatacgtc gataactttg acaacttaga agatatcttc tttaagtttg
    29101 gtctgaatct ttccgatgaa ctgattccac aagtacaaaa agcattacat gaggccatcg
    29161 gcgatattat ggtgccggtc cacaccggca acggcagcat cgatctgatt atccccggcg
    29221 tacataaagc caatggcctt cgccaactgc agaaattatg gggaatagac gacagcgaag
    29281 tggtggtctt tggcgatggc ggtaacgata ttgagatgct gcgtcaggca ggctttagtt
    29341 ttgcaatgga aaatgccggc agcgcggtcg tcgcagcggc aaaataccgg gcaggctcca
    29401 ataaccgtga aggcgtactg gatgtgatcg ataaagtgct taaacacgaa gcgccattta
    29461 accaataaaa cggcagcacg cccggcaaca caatacctga atgaaggctg cataattcgc
    29521 ggaaattaac ccgctgcatt atttcaggcg aaatgattat gaaaaattgt ctactgttgg
    29581 gcgcgctttt aatgggcttt actggtgtgg cgatggcgca aagtgtcacc gtcgatgtgc
    29641 cgagtggata caaagtggtt gtggttcctg attcagtcag cgttccacaa gcggtcagcg
    29701 tcgcgactgt gccgcaaacc gtctatgtgg ctcctgcgcc agctccggct taccgcccgc
    29761 atccttatgt tcgccatctt gccagcgtag gtgaagggat ggttatcgaa caccagattg
    29821 acgaccatca ccattaattc gagaatgcct gatcgcacaa agcaatcagg cattttttta
    29881 cccttcgtgc gaatttccca tctgtttatc ttttaaaaag ataaccatca gcaacagcca
    29941 caaaattccg ttgacaaggt tgaagagatt aaacagtccg ttgcccccgt tcagccagac
    30001 atgtttgctg atttcaatac cgacggtaaa gatcagcatt tgcagcatcc ccatcgccgc
    30061 agaaaccgta cctttactca tatcgctggc aaacagggtt aatcgcacca gtcccgcatt
    30121 cgccagacca ataccgaaag cataaatact taacccggcg gtcatccata aatacgcgtg
    30181 cgatgagata accgttgccg cagcagcgac caacagacca atcataatcg gccagccgcc
    30241 cataataatc agcgaacgta cggtgcggcg cgaggtcaga cgcgctaaca gcaagttacc
    30301 cgcaattaac gccccgaaaa taggcacttg cagcaagcca tattcatagc tgctcaactg
    30361 ctcaccggta ataatgataa tcggcgactg ggcgatccac gccaacaacg gcaggctaac
    30421 gaatcccagc gccagcgccc ccgccacaaa gcggccgttc ttcagcacca gcttatagtc
    30481 acgaccgagt tctttcagcg acagtttctc gcctatacgc gtggcggttt caggcatggc
    30541 tcgttgcaga ccgaaaaagg agatcgctgc caatgcggca aacaagacaa acatcccttc
    30601 ccagggcagc acatggatcc acgccgcgcc caccagcgga ccaagtagtg gagcaatcag
    30661 cgccacgttc gccattagcg cggtgatctt gatacaaacc gcctcttcga aggattcctg
    30721 aattgcggcg tatcccacag cgccaatgaa acagaggctt atgccctgca agaagcgcaa
    30781 cagggtgaat tgttcaatgt tttgcgccag taatattgcc agacaggtga cgataaacca
    30841 caccactccc gccagcatca ccggacggcg gccaatacga tccgacaacg gccccaggag
    30901 ccattgtaaa aacatcccgc ccgccagata cgcagtcatc gaagtaggaa cccaatcaat
    30961 gcccgcctga tattgttcca ccacggccaa cataccgggt tgaatcatat cgttgccgat
    31021 ataggttgaa aattcgtaaa gcaccagaca gagagggaaa agtaacgcct gacgtccaag
    31081 cctggcaccg gaagctaatt tattttgcat gcaatttctt cgccaataat aatcgcgcaa
    31141 agtttaataa aagcgcagct aacgagaaag cgaattttgt agctgaaacc acggttaagc
    31201 acattcttac attattgcga gtatagctac gctttcttta agttttattt aacctttgcc
    31261 cgttacaatc acccaccgta aacaggccgc ttgagggaaa taagacgatg ccgctttacc
    31321 cagtttaacc tgcactttat tctcaacgac ttgcctgtat tggctccctt ttaatcactt
    31381 tgcgtcggga agttatgctg gaaaatttga atctctctct attctctctt attaacgcga
    31441 cgccagactc tgcgccgtgg atgatctcgt tggcgatttt tattgcaaaa gatttgatta
    31501 ccgtggtgcc gttgctggcc gcggtacttt ggctgtgggg gcttacagca caacggcaac
    31561 tggtgataaa aatcgctatc gcgctggcgg tcagcctgtt tgtgtcctgg tcgatggggc
    31621 atctttttcc gcacgaccga ccctttgtcg aaaatatcgg ctataacttc ctgcatcatg
    31681 cggcggatga ctcattcccg agcgatcacg gtacggtgat tttcaccttt gcactggcat
    31741 ttttatgctg gcatcgcctg tggtccggct cacttttaat ggtgctggcc gtcgtcattg
    31801 cctggtcgcg cgtttatctt ggcgtccact ggccgctgga tatgctcggt ggattgctgg
    31861 caggtatgat tggctgcctt agcgcccaga ttatctggca agcgatgggg cataaactct
    31921 atcaacgtct gcaatcgtgg tatcgcttct gttttgcatt accgatccgc aaaggctggg
    31981 tgcgtgactg atctctgcta aaaagtgtag tattgagcgg ctcgcttcaa taactattca
    32041 gagggattat ggaaacacgt cgcgaagagc gtatcgggca gctgctgcaa gaattaaaac
    32101 gcagtgataa gttacatctt aaagacgccg ccgccctgct tggggtttcg gagatgacga
    32161 ttcgtcgcga tctgaacaac cacagtgcgc ccgtcgtttt gctcggcggc tatattgttc
    32221 tggaaccacg cagcgccagc cattacctgt taagcgatca aaaatcccgc ctggtggaag
    32281 aaaaacgccg ggcggcaaaa ctggctgcga cgctggtaga acccgatcag accctctttt
    32341 ttgactgtgg caccaccacg ccgtggatta ttgaagcaat tgataatgaa atccctttta
    32401 ccgccgtttg ttattcgcta aatacctttc tggcgctgaa agagaaaccc cattgccgcg
    32461 cgtttctttg cggtggtgaa tttcacgcca gcaacgccat tttcaaaccc atcgattttc
    32521 agcaaacgct gaataatttt tgcccggata tcgcttttta ttccgctgcg ggcgtgcatg
    32581 tcagtaaagg cgctacctgc tttaatcttg aagagttgcc ggtaaaacac tgggccatgt
    32641 cgatggcgca aaagcatgtg ctggttgtcg accacagtaa atttggcaag gtgcgtccgg
    32701 cgcgcatggg tgacctgaaa cgctttgata ttgttgtgag cgattgttgc ccggaagatg
    32761 agtatgtgaa gtacgcgcag acgcagcgta ttaagttgat gtattaatga cgtataaccg
    32821 gatgacgttt cgcgccatcc ggttatcaga agattaagag aaccagctgc cgaaccactg
    32881 atggaatttc atcatcacga aatcccacat ccgaccaaag aatccgccct cttccacatt
    32941 ttccatcacg atcagcggac gctgctcaat ggatttaccg ttaagctgga aatcaatggt
    33001 gccgacaacc tgaccttttt tcagcggtgc ggtaagctgc ggttccgtta acgtataact
    33061 cgccttcagg tttttcagct gcccacgcgg tatggtcact gagcccgctt cgcctgcccc
    33121 gagattcact tcgctcttat caccaaacca gacgcgctga gtcacaaatg tggcatcagg
    33181 tttaattggc gtcacggttt caaagaaacg gaagccccag gtcaataatt tctcagactc
    33241 attaaaacgg atacggtcag ttttcgcccc cagcactacg gagattaaac gcatatcgcc
    33301 ctgggtagcc gaagcaacca gattatatcc cgcgcctgcc gtggttcctg tcttcatgcc
    33361 atcaacattc agattgctgc tccacagcag acgattacgg ttaggctgac gaattttgtt
    33421 gaaggtgaac tctttctctt tatgaatggc gtactcttcc ggcacatcgt ggatcaatgc
    33481 tttacccagc aatgccatat cgcgcgcggt actgaactgc cccggcgcat ccagaccgtg
    33541 caccgtctgg aaggtagtgt tggtcagacc cagttttttg gcataaccat tcatcagacc
    33601 aataaatgac tcctggctcc cggcaacgta atcagccagc gcaatacagg cgtcattacc
    33661 ggactggata atcacacctt tgttcaagtc tgccaccgaa acctgatcgc ccggtttgag
    33721 gaacattacc gatgaaccac gcagtgccgg atttcccgtc gcccaggcat ctttaccgac
    33781 cgtcaccata tcggtgagtt taatcttatc ggccttaagc gcctgcccaa ccacatagct
    33841 ggtcatgatt ttagtcaggc tcgcggggtc cagtttttca tccgcattgc cttctgccag
    33901 cactttaccg ctggcgtaat ccattaaaat ccatgcacgc gcatccacgc tcggcgcttc
    33961 aacggtttgt tccgccgcga atgccgttgg ggcaaaaagg aataaaaatg cagaacccgc
    34021 tgcaagacca cgaaggagag aggagtattg cgtcataaat gccacccgag tatccattca
    34081 aaaaaaatac gttatatcgc cgtatgataa gttcggtgat taataacgca cacacaaact
    34141 taaagaaacc gtaagttggt aaagttttta aagtttatgc aatatcctgc cgcttcgctg
    34201 cttctggtgt catttttttg ccagctgttg cttaaaagtt aactttactt caccatcgga
    34261 gtcctgttca gcctgtggct gcctgtcgga aaaggggtga gtatgattac gctgtggggt
    34321 cggaataatt caaccaacgt aaaaaaagta ttgctgacgc tcgaagaact ggaactacct
    34381 tatgagcaaa ttctcgcggg ccgtgagttt gggataaatc acgatgctga ttttctggcg
    34441 atgaacccta acgggctggt gccgttgttg cgtgacgacg aaagtgatct cattctttgg
    34501 gaatcaaacg ccattgtccg ctatctggcg gcacagtacg ggcaaaaacg cctgtggatc
    34561 gactcaccgg cacgtcgtgc ggaagccgaa aaatggatgg actgggcaaa ccagacgctc
    34621 agtaatgctc atcgcgggat cctgatggga ttagtcagaa caccaccgga agagcgcgat
    34681 caggccgcca ttgatgccag ttgcaaagag tgcgacgccc tgtttgccct gctcgatgcg
    34741 gaactggcaa aagtaaaatg gttctcaggc gacgagtttg gtgtgggcga tatcgctatc
    34801 gcaccgttca tctacaattt gtttaacgtt ggcctgacct ggacaccgcg tccaaatctg
    34861 caacgctggt atcagcaact cactgaacgc cccgcagtgc gcaaagtggt gatgattccc
    34921 gttagctaat tgcgtgggct aactttaagt aattccccac tggactcgtc ggtgagaacg
    34981 tataaataac cgtcgggtcc agtgcgaaca tcacgaattc gctgccctct gtccgttaaa
    35041 atacggccat cttctgtcac tttgtcgccg ttgacgctca tcacaatgac atctttatct
    35101 ttcagcgcgc caataaataa tttttgctgc cactggggga atttgtcgct gttatagaag
    35161 gccatgccgc tcacggcggg cgaatctttc cagtaaaaaa caggttgctc ggtcccggcg
    35221 acgatctccc ctttcgcctc cggtatctta aagcctgaat agttgattcc ccaggttgcc
    35281 agcggccagc cgtagttttt gcctttttgc gggatattaa tttcatcgcc accgcgcggg
    35341 ccatgttcat tcagccatag tgcattactc cacggattca tcgccattcc ttgcggatta
    35401 cgaatgccat aagaccagat ctcggcgcgt gcatcggatt cctttataaa aggattatca
    35461 tccgggattt ctcccaggtc ggtcagtcgc accagtttgc cctgtaattt atccagatcc
    35521 tgcgccgtcg ggcgctgatt gttttcgccc agagcaataa aaagataacc tttaccgtca
    35581 aataccatcc gcccgccaaa atggttgccg gtagacagtt ttggcatctg acgaaagacg
    35641 gtgcggaagt cggtcacttt tgagagatca tcacttaagc ggccataacc cacagcagtt
    35701 ccggctttgc catcatcgcc aacttcggaa taacttaacc agatgcggcg cgactgagca
    35761 aaatcgggcg ctaaaaccac gtccagcagg ccgccctgcc cgtgcgccca aacgtccggc
    35821 actccggaaa gcggcgcaga taatcctttt cctgcttgcc agtgacgcaa ctcgccgccg
    35881 cgcagagtga ttaacatacc gtgattgtcg ggtaaaaagg ccagcgccca gggatggtcg
    35941 agtttgtcct gcagtacttc gacatttacc gttgcaggag ccgcccagag agcggaagaa
    36001 agacaaataa ggggcacaag gaaaaaggat tgtcgatgca taacgcctcc ggattgactc
    36061 tggcttaaag cgtagtcagt ggaggagata acaaatttat ttttacaaaa acttaaacat
    36121 gaagggggag acgctttctc ccccttagtt ttcaggcctt ctcaagcatg gcgtgcttct
    36181 gcaggctctg gatactcagc gttaagctca tcagacaatt ttcaagctta tcggcgttga
    36241 cggtaataac agtcgggcaa tcatggtgcc cactcatcaa acatactgcg gctgtcgcta
    36301 atgcttcttc agcatgatga agagcactcc actcttcctg atccagatga agattcaacc
    36361 gcagcgattt atcgtgcagt tcgcgattca gtttaaaaaa gttatctcgt agatgattgc
    36421 tttcgctgac ggacatgtat ccttttgcct tcctcagttg cacgtatgcg gcgaggtcaa
    36481 cgcgtgcgtc gatcaaccgg ttcagcagat cgattcgctg tctgtcaacg aacatacttc
    36541 gatcctcctc tttccagcct tgttgcaatt aaatagtatg gctatttttt gagcaaaatg
    36601 ctgtctagga tcaattcttc acgccgaatt aacaaaattt acatttcctt cgcctccgcc
    36661 agcgaatgag attgcgatac aaattttatc cagaactctg tagaatccct gccctgacct
    36721 ttactgtaac tgaatatttt ttaagctatg agcaaagtaa ctccccagcc gaaaatcggc
    36781 tttgtttccc ttggctgccc gaaaaacctt gtcgattcag agcgtattct caccgaacta
    36841 cgcactgaag gttatgacgt ggtaccgagc tatgacgatg cggacatggt gatcgtcaac
    36901 acctgcggct ttattgacag cgcggtacaa gaatcactgg aagccattgg tgaagcgttg
    36961 aatgaaaacg gcaaggtaat tgtgaccggt tgtctggggg caaaagaaga tcagatccgc
    37021 gaagtccacc cgaaagtgct ggaaatcacc gggcctcata gctatgagca ggttctggag
    37081 cacgttcatc actacgtgcc aaaaccgaaa cacaacccat tcctgagcct ggtgccagaa
    37141 caaggtgtga aactgacgcc gcgtcattat gcctatctga aaatttctga aggctgtaat
    37201 caccgctgca ccttctgcat tattccgtct atgcgcggcg acctggtgag ccgtccgatt
    37261 ggcgaagtgt taagtgaagc gaaacgtctg gttgatgcgg gcgttaaaga gattctggtg
    37321 atctcgcagg atacttccgc ctatggcgtc gatgttaaac atcgtactgg cttccacaac
    37381 ggcgagccgg taaaaaccag catggtcagc ctgtgcgaac agttatcgaa actggggatc
    37441 tggacacgtc tgcactacgt ttacccttat ccgcatgtgg acgacgtcat cccactgatg
    37501 gcagaaggca aaatcctgcc ctatctggac attccgttgc agcacgccag cccgcgcatt
    37561 ctcaaactga tgaagcgtcc gggttctgta gatcgccaac tcgcgcgcat caaacagtgg
    37621 cgcgaaatct gcccggaact gaccctacgc tcaaccttta ttgtcggctt ccctggcgag
    37681 acggaagaag atttccagat gctactcgac ttcctgaaag aagcacgtct ggatcgcgtt
    37741 ggctgcttta aatacagccc ggttgaaggt gcagacgcca atgccctgcc tgaccaggtt
    37801 ccggaagaag tgaaagaaga acgctggaac cgtttcatgc agttgcagca gcagatttcc
    37861 gccgagcgcc tgcaagagaa agtgggccgt gaaattctgg tgattatcga cgaagtggac
    37921 gaagaaggtg cgattggtcg cagcatggca gatgcaccgg aaatcgacgg cgcggtttat
    37981 ctcaatggtg aaaccaacgt taagccgggt gatatcctgc gtgtgaaagt cgagcacgcc
    38041 gatgagtacg atttgtgggg tagccgggtt taattaatct taaggcagat ctaacaagga
    38101 tattgttctt cgatctgcct ctgctaccct tcccgatgtc ctacccttac gcacaaaatc
    38161 ccctgacgca taaatcgaga aaaatgaatc gtactcgagg caactctcac tttttcacac
    38221 caaattaaag ttttgatcgc aattacttga aataagcgta atggttacta tagtaaacat
    38281 taccacaagg atggatgtat gggtgtgtat aaggcaagac gattcagcca aagcacgaaa
    38341 aaacttggta ttcacgataa agttctcatg gctgccgctg aagaagtgat tcagggcata
    38401 tgggaggctg atttaggtag cggtgtcatc aaaaaacgtt tacccctcca acaaggaaag
    38461 agtggtggag ccagaacaat tatctttttt aaatcagcaa atcatgtgtt tttttatgat
    38521 ggttggtcca agagtggtct aagcagtaaa ggaaccaaag aaatcgaaga tgatgaactg
    38581 gcggcctaca aaaaaatggc aaatgcattt ctggccttta gcaataagca aatagaggat
    38641 ttgatcgaaa ctggcttttt gattgaggtg aaaaatgagc gataaattac aaatgatgct
    38701 ggaagatgca aaggaactga atgcactggg cctgatgtct gatagtgaag ttgatgccat
    38761 tgccgtcatg gtcaaagctc gtgaagtcag cgcccgtata gccaaagtca aatcaatgac
    38821 gggtgaagag atcaaagcca tccgcacccg atacggcatg tcacaatccg tgctggcaca
    38881 tacaatggga atgtcaaaag aaagtgtctc gaaatgggaa cgtaatgaaa taaaaccgag
    38941 cggcccggca ttacgtattt tgaatacatt ggcaataaaa gggccggaag tttttgcaaa
    39001 gtaaaatgtt tgcaaatgat ttaccacatt cattagtacc ccttgattaa ggggtactaa
    39061 cactattcta attctactgc cagctaatct gcgccctgcc gtttttctta ctcttataca
    39121 gtcgctcatc cgccagcatt ttcaagcgtt cgacggtagt acattcattt gaatgcgcta
    39181 caccgatact ggtgcttaaa ttaatgcttc ccacctggaa atgaacaatt tcttttagaa
    39241 tcgatagcaa ctcttccagc gttccacccg tataaatcac cgcaaattcg tcaccgccga
    39301 agcggtagac tttaccttta tctacgggga gctgtgattt aaggattttc accactttca
    39361 tcaacacttc atcgcccagc aaatgaccga aggtgtcgtt aatgtttttg aatttatcga
    39421 cgtcaaacag gcacaaataa ccgccttttt ccggcagcgc ttttaaatct tcgtcaaagg
    39481 attttctgcc catcgctttg gtcagatcgt ccaggaatgc cgagttatac atactgtaga
    39541 gttttttgac atcggcagcc gaaaagaaaa gcccgccatc cagtacgcgt tcaaaatggt
    39601 tattaataat ggcttctttc tccacaatac cacgattaat cactctgcgt accagataga
    39661 ggcaactggc acccgatatc actgccacca atgcggcaaa gaaaataatc aatgtacata
    39721 cctgaaaata aacgtctttt ttataagcaa atacgtacaa cccggtcggc aaataggcga
    39781 agtgctccat cgcttccgaa acagtgaaga tggcgttttt agcgttatat tcatcaccgg
    39841 tcaccgattt accgttcttg aacagggaga acatcaggtt ctcgctatcc atcgccagcg
    39901 attttgacag taaagaaaaa cgttccagac caagcaaaaa tatcagacta cgattgtgag
    39961 ttaccggata gaggtaatag acgaaggttt taccatcaaa agagataaac cctgtatttt
    40021 tgacatgtcg atacttcaca acctgcataa tatctgcaca ataatttttc tcgaactcct
    40081 ttacctgaat acattcgtca ctggaaatca gataaggacc gacatcgtac aaataagagg
    40141 gtgaaaccga gtcggcgtaa gctgtgcgta agccaaccaa cagctgttcc tccgtgggat
    40201 ttagcgtgtt gataagatca attgccacgc tacgccgata ttcagcaata tttgccaccg
    40261 caaaagcagt atcaataacg gactgtttca ggctatacac ccgttccttt accatttgag
    40321 tgtagatccc gcaggcaact gctgaaatca tgataaaaat cagcagactg actgtaagta
    40381 cgaacttatt gattctggac atacgtaaac tactctttta ctaattttcc acttttatcc
    40441 caggcggaga atggcatggg tttctggtac agatacccct gagcctgaac acatcctaat
    40501 ttgattaatt cgcgcgcaac gtcttcgctc tctacgcctt ccgcaatcac cgggatccgg
    40561 ttatagcgtg ccagatcgaa aataatgcgc acaatttttc ccgcagtacc aaaaatatcg
    40621 ccggtaaact gcgagtccac ctttatgctg tctggattaa tttcataaaa acgtttcagg
    40681 tttgacaaac cggtgccaaa gtcatcaatc gatatccgta ccccttgttt acgcagttgt
    40741 tcaatattga gataaaaatg ttcattaatt cgctcaatca cggtttcagt gatctcaaca
    40801 caaaggctgt tcgccttcag gccattttct ttcatgatgg tagtaaaacg ctcatgaaag
    40861 tgtggttcgt ttaactgcca gggcgaaaca ttaatatgca gtaagaaatc atccttcagc
    40921 gacgcacgct gactaaaatg tctgaattca cccatcgcgg ttttaataat ttgatagccc
    40981 agctcattga taaacccgat atcttcagca atggtaatga acttcagcgg tgaaatgatc
    41041 ccccgctccg cagacaccca acggcacaac gcctccgcgc ccagaatggc gcgggtgtta
    41101 atatcaacga tcggttgata gacgaggaat agttcgccaa tttccattgc atggcgaata
    41161 ttacgcgcaa tccagatatt ctcttctatt tcgttgagtt tcgcatcatc acagaaaata
    41221 aactctgaga acatatggtt gcggatactg gaaatcgcca gtttcagctt ttctgcgtaa
    41281 ctccaggcgt tttccccctg caaaggttca caaatagcag acttatggat gcgcagattg
    41341 cggtagccaa aactgctgtc aaaatcgttt accgtcgcta tcttacgggt aaacgtctgt
    41401 acattttccc ggcacaggag taaatagaga tcggcattaa atgtcacgat atcgcagcat
    41461 tgcgcatatt tctcggcaat tcgctgacac aatttcctga cgattttatt gcgttcatca
    41521 acaccaaaca ccgcttccac agcttccaga ccacacagtt agatgatccc cacagccaga
    41581 tactgattat tgttggattc aataatcgcc atatttttaa taaaaccgct ttcctgatcg
    41641 taagagatca gtgagagcaa tttatttgac cgttcaattt cggcatcttt catttccgcg
    41701 atcgaatttt tgatgctgat aatctctttc gcatcgcctt cgcgaatttt cagagcctcg
    41761 atctgatccg ttgagtcggg caaaaacgcc agcgtattaa ccagctcggt catttcagaa
    41821 aattgccgac ggaacacacg agtggtcatt aaataaacca ccaacaatac gataataatg
    41881 aacgcaaacg gcagataaaa aagtttatct ga
//
LOCUS       DAZGQL010000035        41718 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.035, whole genome shotgun sequence.
ACCESSION   DAZGQL010000035 DAZGQL010000000
VERSION     DAZGQL010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41718
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.035"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            63..311
                     /gene="ymgC"
                     /locus_tag="ACHKIR_002142"
     CDS             63..311
                     /gene="ymgC"
                     /locus_tag="ACHKIR_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgC"
                     /protein_id="HFO9989884.1"
                     /translation="MNNSIPERFILQCALFKNLEREVFMTHGYVDSYIIDQALRLRLK
                     DETSVILSDLYLQILQYIEMHKTTLTDIIINDRESVIS"
     gene            647..2170
                     /gene="pdeG"
                     /locus_tag="ACHKIR_002143"
     CDS             647..2170
                     /gene="pdeG"
                     /locus_tag="ACHKIR_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFO9989885.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECDLEEQYQLGTEAALKPHLRTIIILKQGTVWCTSLP
                     GNRVLLSRIPVFPDSNLLLAPAIDTVNRLPILLYQSQFADTRILVTISDQHIREALNV
                     PLKGVRYVLRVADDIIGPTGDVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIACAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTRSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNYRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLDYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
     gene            2302..2520
                     /gene="ymgF"
                     /locus_tag="ACHKIR_002144"
     CDS             2302..2520
                     /gene="ymgF"
                     /locus_tag="ACHKIR_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division-associated protein YmgF"
                     /protein_id="HFO9989886.1"
                     /translation="MNNSNNLDYFTLYIIFSIAFMLITLLVILIAKPSTGLGEVLVTI
                     NLLNALVWLAINLINRLRERLVSHRDQH"
     gene            2918..5560
                     /locus_tag="ACHKIR_002145"
                     /pseudo
     CDS             2918..5560
                     /locus_tag="ACHKIR_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768537.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ECIAI39_1904 family autotransporter adhesin"
     gene            complement(5618..5953)
                     /gene="ymgD"
                     /locus_tag="ACHKIR_002146"
     CDS             complement(5618..5953)
                     /gene="ymgD"
                     /locus_tag="ACHKIR_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HFO9989887.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(5957..6301)
                     /gene="ymgG"
                     /locus_tag="ACHKIR_002147"
     CDS             complement(5957..6301)
                     /gene="ymgG"
                     /locus_tag="ACHKIR_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HFO9989888.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            complement(6303..6476)
                     /locus_tag="ACHKIR_002148"
     CDS             complement(6303..6476)
                     /locus_tag="ACHKIR_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9989889.1"
                     /translation="MSYLSFEIILIPVKNIIPIITVTLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            6577..6762
                     /locus_tag="ACHKIR_002149"
     CDS             6577..6762
                     /locus_tag="ACHKIR_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9989890.1"
                     /translation="MSRKYSSAMITLVILFCRRPHCRKISVQQYYQKRVIFYPTTLQR
                     TAFKKCLFLMRDIIISS"
     gene            6723..6842
                     /locus_tag="ACHKIR_002150"
     CDS             6723..6842
                     /locus_tag="ACHKIR_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFO9989891.1"
                     /translation="MPLSYAGYHYFIMMSLVSGEHNTLALPIQEQSPGLVPAG"
     gene            6877..7221
                     /locus_tag="ACHKIR_002151"
                     /pseudo
     CDS             6877..7221
                     /locus_tag="ACHKIR_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(7586..7852)
                     /gene="minE"
                     /locus_tag="ACHKIR_002152"
     CDS             complement(7586..7852)
                     /gene="minE"
                     /locus_tag="ACHKIR_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HFO9989892.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(7856..8668)
                     /gene="minD"
                     /locus_tag="ACHKIR_002153"
     CDS             complement(7856..8668)
                     /gene="minD"
                     /locus_tag="ACHKIR_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HFO9989893.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            complement(8692..9387)
                     /gene="minC"
                     /locus_tag="ACHKIR_002154"
     CDS             complement(8692..9387)
                     /gene="minC"
                     /locus_tag="ACHKIR_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HFO9989894.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            9906..10274
                     /gene="ycgJ"
                     /locus_tag="ACHKIR_002155"
     CDS             9906..10274
                     /gene="ycgJ"
                     /locus_tag="ACHKIR_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HFO9989895.1"
                     /translation="MNMMRIFYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVN
                     GKRSGKINQTTTKMLFMCRE"
     gene            complement(10377..10778)
                     /gene="pliG"
                     /locus_tag="ACHKIR_002156"
     CDS             complement(10377..10778)
                     /gene="pliG"
                     /locus_tag="ACHKIR_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="g-type lysoyme inhibitor PliG"
                     /protein_id="HFO9989896.1"
                     /translation="MKIKGISKAVLLLALLTSTSFAAGKNVNVEFRKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            11020..11313
                     /locus_tag="ACHKIR_002157"
     CDS             11020..11313
                     /locus_tag="ACHKIR_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HFO9989897.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKQALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            11385..12044
                     /gene="ycgM"
                     /locus_tag="ACHKIR_002158"
     CDS             11385..12044
                     /gene="ycgM"
                     /locus_tag="ACHKIR_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFO9989898.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            12136..12582
                     /gene="ycgN"
                     /locus_tag="ACHKIR_002159"
     CDS             12136..12582
                     /gene="ycgN"
                     /locus_tag="ACHKIR_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HFO9989899.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(12789..13115)
                     /gene="hlyE"
                     /locus_tag="ACHKIR_002160"
                     /pseudo
     CDS             complement(12789..13115)
                     /gene="hlyE"
                     /locus_tag="ACHKIR_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
     gene            13376..13795
                     /gene="umuD"
                     /locus_tag="ACHKIR_002161"
     CDS             13376..13795
                     /gene="umuD"
                     /locus_tag="ACHKIR_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFO9989900.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            13795..15063
                     /gene="umuC"
                     /locus_tag="ACHKIR_002162"
     CDS             13795..15063
                     /gene="umuC"
                     /locus_tag="ACHKIR_002162"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HFO9989901.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVHNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDEVWGIGRRISKKLDAMGIKTVLDLADSDIRFIRKHF
                     NVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYPSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFAFNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPCYTTRSSDLLRVK"
     gene            complement(15108..15638)
                     /gene="dsbB"
                     /locus_tag="ACHKIR_002163"
     CDS             complement(15108..15638)
                     /gene="dsbB"
                     /locus_tag="ACHKIR_002163"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HFO9989902.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMARFPEWLPLDKWVPQVFVASGDCAERQWEFLGLEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(15784..17325)
                     /gene="nhaB"
                     /locus_tag="ACHKIR_002164"
     CDS             complement(15784..17325)
                     /gene="nhaB"
                     /locus_tag="ACHKIR_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HFO9989903.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            17547..18266
                     /gene="fadR"
                     /locus_tag="ACHKIR_002165"
     CDS             17547..18266
                     /gene="fadR"
                     /locus_tag="ACHKIR_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0019217 - regulation of fatty acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HFO9989904.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(18318..19850)
                     /gene="ycgB"
                     /locus_tag="ACHKIR_002166"
     CDS             complement(18318..19850)
                     /gene="ycgB"
                     /locus_tag="ACHKIR_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HFO9989905.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSVELLERCPPRMG
                     NL"
     gene            20180..21478
                     /gene="dadA"
                     /locus_tag="ACHKIR_002167"
     CDS             20180..21478
                     /gene="dadA"
                     /locus_tag="ACHKIR_002167"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HFO9989906.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            21488..22558
                     /gene="dadX"
                     /locus_tag="ACHKIR_002168"
     CDS             21488..22558
                     /gene="dadX"
                     /locus_tag="ACHKIR_002168"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HFO9989907.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPYARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISSAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(22615..24351)
                     /gene="cvrA"
                     /locus_tag="ACHKIR_002169"
     CDS             complement(22615..24351)
                     /gene="cvrA"
                     /locus_tag="ACHKIR_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006884 - cell volume homeostasis [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HFO9989908.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(24446..25360)
                     /gene="ldcA"
                     /locus_tag="ACHKIR_002170"
     CDS             complement(24446..25360)
                     /gene="ldcA"
                     /locus_tag="ACHKIR_002170"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HFO9989909.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAHGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTQEGTQLTISGHPVLKM"
     gene            25460..26071
                     /gene="emtA"
                     /locus_tag="ACHKIR_002171"
     CDS             25460..26071
                     /gene="emtA"
                     /locus_tag="ACHKIR_002171"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HFO9989910.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(26073..26807)
                     /gene="ycgR"
                     /locus_tag="ACHKIR_002172"
     CDS             complement(26073..26807)
                     /gene="ycgR"
                     /locus_tag="ACHKIR_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HFO9989911.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRISWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNNAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AGLHEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            27008..27262
                     /gene="ymgE"
                     /locus_tag="ACHKIR_002173"
     CDS             27008..27262
                     /gene="ymgE"
                     /locus_tag="ACHKIR_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFO9989912.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            complement(27312..29282)
                     /locus_tag="ACHKIR_002174"
     CDS             complement(27312..29282)
                     /locus_tag="ACHKIR_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFO9989913.1"
                     /translation="MRLKKRYLCTALSLAFTQQALAAQESDTLTVWSSPVSSTTTTVL
                     DHPTMKALDKQNVAQALSVVPGVVLQKSGSRNEEQVKVRGFDSRQVPVYFDGVPIYVP
                     YDGNLDLARILTNNLGAVEVSKGYSSLLQGPNQMGGAINITTQKPTKPLEANLGYRQG
                     WSRSQDNAYDMHASFAASSDLGYLQVSGSQLKQDFLGLPHGVNNDIAGKHGKMINSSA
                     DDKRGIVKLGFTPRENDEYTLTYIKQDGEKDNPPYSGNSGQKSRYWQWPEYDKESFYY
                     QGTTQLNDRFTLKSRLYRDTFENTLMMYNSLADLKNKKGSYSHYSDYSDGAGLQLAAD
                     VRENDLLSFAVNWKDDVHREKGAPHAAYDRYEDRTWSLASEYQWAAADNVDVVAGISY
                     DWRDSVEAKKHEKDGSITHYDDNNQSAFNWQVMGKYHFANEDTLALSYYDRTRFPTLK
                     ERYTTSKPAYNQIAIVNPQLKPERARGVDLTWNGAFTHDWGFEVSVYYNRVSDAILSH
                     NIDADTIQNQNSGTVDYSGLDAGIKGKISNILDVGLSYALIHADAKRKDIGKITDLPT
                     QTMTAWMTLKPWEPLSVTLSEEARSSSYSNSDGSQKAAGFAVTHIRADYTLGHGFSVN
                     ASVNNLFDTKYAYSEGFIEEGRNFWAGIEYTF"
     gene            complement(29308..30162)
                     /gene="modD"
                     /locus_tag="ACHKIR_002175"
     CDS             complement(29308..30162)
                     /gene="modD"
                     /locus_tag="ACHKIR_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HFO9989914.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQHGYIEFFHRQGG
                     CVSGISVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAVQNVLEW
                     SCGVSDYLAQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQDWSGAINQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     NPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYATPADIKV
                     SLQPAASI"
     gene            complement(30159..30971)
                     /locus_tag="ACHKIR_002176"
     CDS             complement(30159..30971)
                     /locus_tag="ACHKIR_002176"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000903984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO9989915.1"
                     /translation="MLIDDIDFADLYLQQLRLAHRTEKTPDHWDQRAEKMAENCASPT
                     DSYLQQLTSKIDLQGAQTLFDMGCGPGTVSLALADKLTTIYGVDYSQGMLNVAARRAA
                     ALKADNVHWIQRAWEEDWSDLPRCDIAVASRSTLVADMRQAMSKLNNQARLRVYTTHL
                     VSTSFVSPAIQRAAGREVVELPNYIFALNVLYQMGIYAHVDFIRGQNCQQDNSTWERF
                     EQNVSWSLGALNDDERERLYRWYQQQDARALAPASRDWALIWWDSVPQEALR"
     gene            complement(30981..31739)
                     /locus_tag="ACHKIR_002177"
     CDS             complement(30981..31739)
                     /locus_tag="ACHKIR_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO9989916.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEILLDSLPVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGQ
                     APTIGAFSVPGKQERLKATEQLEKLGILHLATRRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHPLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTKKLAALYRVSADQIHHHLSAIKH"
     gene            complement(31736..32716)
                     /locus_tag="ACHKIR_002178"
     CDS             complement(31736..32716)
                     /locus_tag="ACHKIR_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFO9989917.1"
                     /translation="MRTVNGCILLLAAISITFAAVSGAYHLDMQQLLALILRQENVTV
                     QEQIVFWQIRVPRILAALFLGAALAGAGTTYQGMFRNPLVSPDILGVSAGAGLGACAA
                     ILWGLSIVYIQLYAFCGGLMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISL
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFGLIVCATLITASTVAIAGIIGWVGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLFLLLRGGRQ"
     gene            complement(32716..33738)
                     /locus_tag="ACHKIR_002179"
     CDS             complement(32716..33738)
                     /locus_tag="ACHKIR_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HFO9989918.1"
                     /translation="MSLTRRRFTQILASTLFLHHLPSFAQSVKFWASLTLPEAQNITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLPEHIRQFPRLGRLAGRASTLSL
                     EGLMALHPDLVVDCGNTDETWISQARQVSEQTQIPWLLLNGKLEQSAEQLTTLGKTLG
                     EEHRAAEQANLASRFVGEAQAFATSPAANLSFYAARGPRGLETGLQGSLHTEAAELLG
                     LHNVAQIADRHGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWQGVKAVAEQRIL
                     FLSGLPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCPLSDADY
                     QKLVAS"
     gene            complement(34075..35772)
                     /gene="treA"
                     /locus_tag="ACHKIR_002180"
     CDS             complement(34075..35772)
                     /gene="treA"
                     /locus_tag="ACHKIR_002180"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFO9989919.1"
                     /translation="MKSPAASRPLKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEIDTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRASKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATRPLSESTTQPVKQNEAEPTP"
     gene            complement(36093..37511)
                     /gene="dhaM"
                     /locus_tag="ACHKIR_002181"
     CDS             complement(36093..37511)
                     /gene="dhaM"
                     /locus_tag="ACHKIR_002181"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019563 - glycerol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFO9989920.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDNCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADTDHVLVMMDIGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGAEIDKVIFDAMHALEAKREQLGLPSSNTEISDTCPSYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPILRPVPPVSGKAFYYQPVLCTVQAKSTL
                     TVDEEQERLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLDDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTILQLDPAVVKGICLSAGSPLSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFSRQG"
     gene            complement(37522..38154)
                     /gene="dhaL"
                     /locus_tag="ACHKIR_002182"
     CDS             complement(37522..38154)
                     /gene="dhaL"
                     /locus_tag="ACHKIR_002182"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFO9989921.1"
                     /translation="MSLSRTQIVNWLTRCGDIFSTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSIPAALEAASS
                     VAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            complement(38165..39235)
                     /gene="dhaK"
                     /locus_tag="ACHKIR_002183"
     CDS             complement(38165..39235)
                     /gene="dhaK"
                     /locus_tag="ACHKIR_002183"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415718.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFO9989922.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRSDAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECAMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLENGSYHRTLRFWDYQQGSWQEESQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            39463..41382
                     /gene="dhaR"
                     /locus_tag="ACHKIR_002184"
     CDS             39463..41382
                     /gene="dhaR"
                     /locus_tag="ACHKIR_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001467850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase operon transcriptional
                     regulator DhaR"
                     /protein_id="HFO9989923.1"
                     /translation="MSSAFNNDGRDISPLIATSWERCIKLMKRDTWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRACALLILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQEDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLHSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRG"
     gene            complement(41419..41595)
                     /locus_tag="ACHKIR_002185"
     CDS             complement(41419..41595)
                     /locus_tag="ACHKIR_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016158417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9989924.1"
                     /translation="MKKTFTFSLLAIVFASLLSACVPHHHHRHSDGPRGPAPSGKPMP
                     PSNGGPNHGSHGRY"
BASE COUNT        10555 a         9996 c        10572 g        10595 t
ORIGIN      
        1 gtctgtcgct ttagactata cacagcataa ttttattggg ttaatatttc tacgaggctg
       61 acatgaataa ttcaatccca gagagattta ttttgcaatg tgctttgttt aaaaatctcg
      121 agagggaggt gttcatgacg catgggtatg ttgatagtta tattattgat caggctttac
      181 gcttgcgttt aaaagatgaa accagtgtga ttctttctga tctctatctg caaatattgc
      241 agtacattga aatgcataag actacactaa cggatatcat tattaatgac agggaatccg
      301 tgatctctta gaacttgagt aaggttatag aataaacatc agctgtatca ccatgctggt
      361 gcaaagtgag catttcaggc gttatgcttt cttaatatat ccgcaatatc agggggcaaa
      421 caggaatgga gagcgctcgt ttttcattct tgacgcctga tatcccacct aatttgttta
      481 attctgccta cattgtgctc aataattaat aggttagatg aagttggaga aaattttatc
      541 ttcggcgtgg atatttgtga tgctctctac catgctctct gtagacgcca gcgcgagcga
      601 atgaacgtca ttttcatgta ttaaaccctt tgtccgggac aagcgaatgc gcaatacact
      661 catacccatc ctcgttgcga tatgcttgtt tataaccggt gtcgctattt tgaacatcca
      721 gctctggtat tccgcgaaag cagagtacct ggcgggagcg agatatgccg ccaacaatat
      781 caatcatata cttgaagaag cgtcacaggc gactcaaacg gcggttaaca ttgccgggaa
      841 ggaatgcgac ctcgaggagc aatatcagct tggcactgaa gcagctctga agcctcacct
      901 gcgcacaatc atcattctca aacagggaac ggtctggtgt acatccttac cggggaatcg
      961 ggtcctactg tctcgtattc ctgttttccc ggacagtaat ttactgctgg ctccggcaat
     1021 cgacaccgtt aatagattac ctatcctgct ctatcagagc caatttgcag atacgcgcat
     1081 tttggttacg ataagtgatc agcatattcg cgaggcgctt aatgtaccct tgaaaggggt
     1141 aaggtatgta ttacgcgtgg cagatgacat tattggacct acgggtgatg tgatgacgct
     1201 taatggacac tatccctata ccgagaaggt tcactctaca aaatatcatt tcactattat
     1261 ctttaacccg ccgccactct ttagcttcta cagacttatc gataaaggct ttgggatttt
     1321 gatatttatt ctgttaatcg cctgcgccgc tgccttcctg cttgataggt atttcaataa
     1381 aagcgcaacg cctgaagaga tcctgcgacg ggctataaat aatggggaga tcgtcccttt
     1441 ttaccaacct gtggtaaatg gtcgggaagg gacattgcgg ggagttgagg tgttagcccg
     1501 ctggaaacaa cctcacggtg gatatatatc acctgcggca tttattccac ttgctgaaaa
     1561 atccggatta atcgttccgc ttacgcgaag cctgatgaat caggttgcca gacagatgaa
     1621 cgctatcgcg agtaaattgc cggaaggttt tcatattgga attaatttta gcgcctcgca
     1681 tattatttcg ccgacgtttg tcgacgagtg cttaaattac cgtgacagtt ttacccgccg
     1741 cgatttaaac ctcgttctgg aagttaccga gcgtgagcca ttaaatgttg atgaaagtct
     1801 ggttcagcgg ttgaacattc tgcatgaaaa tggttttgtc atcgcgctgg atgatttcgg
     1861 tactggctac tcagggcttt cttatctgca tgacctgcat attgattata tcaaaattga
     1921 tcacagtttc gttggccgcg tcaacgcaga cccagaatca acccgaattc tggattgtgt
     1981 attggatctg gcgcgtaaac tttcgatcag tatcgtcgct gaaggtgtcg aaacgaaaga
     2041 acaacttgac tatctgaatc aaaataatat cacatttcag cagggttatt atttctataa
     2101 acctgttaca tacatcgacc tggtcaaaat tatcctttct aaaccgaagg tgaaggttgt
     2161 ggttgagtga aaatcgatca gtaaggccat agtgcggtgt aattatagac agctaattag
     2221 ctcgttgcct cttgttacta ttgttcatta ttttgtttgc tataattgtt tgaaagtttt
     2281 gacaggattg ccattagtag catgaacaat agtaataatc tggattattt cactctctat
     2341 atcatctttt ccattgcatt tatgctgatc actctcctgg tcatccttat tgcaaaaccc
     2401 agtaccgggc tgggagaagt gcttgtgacg ataaatttgc ttaatgccct tgtttggctg
     2461 gcgatcaatc tgattaatcg attaagggaa agactcgtaa gccacaggga tcagcactaa
     2521 tctttcagtt tctcactgtc agtatgcggc tgaatgggtt gctggcagtg aacgcctgga
     2581 tcattgaaga aaggaattat tgcgcaaata gttgtcaact ctggtgttat cacagttgtt
     2641 ttatatatca ttgaaataat ttctatggca actattaatg gttttgatgt cgaagagtta
     2701 tttgttaaac aaaatcgtca ccccaaagtg atcaatgtca tgaaaataag gtgaaaaatg
     2761 ataatgccgc cttatttatc atttatatat tgtcgctgtt tatctattta aattttttgt
     2821 gcttttgttt ttgtctttac cctccgtttt ggtaatctga actcataggt tatttgacca
     2881 ggaatgtttg ttaatccaat gaatggatgg ttaatctatg aaactgaaaa aactccccgg
     2941 gtttagttta ggacttattg ctttggcggt gggtaatgca tatgctgaac agtttattca
     3001 gcacgatgaa cctaaacatg ttgttgaaat aaaagatgga gaagtaaaag atattgataa
     3061 tgcgctagtt ataacaacgg gtaatggaag ttacggtgta tctgttgcag gaaagggtag
     3121 caatttaacc attaacaatg gcgtcattgt aacaacgggc ggtttatatc cttctgacaa
     3181 tagttctcta agcacagcat atacagctag cgcagtcgta agtgaatatg gcggtgctgt
     3241 cactttgcgg ggtaataata cgattgtcac gacgggagat tattctgttg gtttgttaag
     3301 tcaagttgat ggaaacctga atactgatac cataataaga gtcaaccctg acggttcggc
     3361 taccccctct ttttctgacg gaggtaatac ttttatcgtt actgcgggta atcacgcagt
     3421 cggcgttctt gcgtgtgcat ctcccggaag cgcgcgtgcg tgtgtatctt ctcttgatga
     3481 agaaagtact gccgatacag gaagtaatga aaataacgcc aaagcaaaac tggatatggc
     3541 aaaaggggag ataacaaccc acggaacaga aagttatgcc gcttacgcta atggcactgt
     3601 cgtcaaggag ggtagcaagc ttgattatac caatgccagc gttacattaa ccgatgttga
     3661 tatcaccacg catggggaca atgctcatgc catcgctgct cgccagggga cagtttcatt
     3721 taaccaggga gaaatttata caacgggtcc tgacgccgcg attgctaaaa tttataatgg
     3781 cggcaaggtg acgctgaaaa atacatctgc agtcgcgcat cagggggctg gaattgtgct
     3841 ggagtcttcc ataaatggtc aggaagcaac ggtagatatt ttatctggta gctcactacg
     3901 gtcagcaaat gaaatcctct accataaaaa tgagacgagt aacgtgacca ttacggatag
     3961 tgaggtatca tcggctgcag atgtttttat taataatatt aaaggtcatt tgaccgtcga
     4021 tgcaactaat tcaaaaataa cgggttcagc caatctttca acagatgata acactcatac
     4081 ctatctgtcg ctttcagata atagtacctg ggatattaaa gctgactcaa cggtgagcaa
     4141 actcacggtt gataatagta cggtctatat ttcccgggca gatggaaagg atttcgaacc
     4201 aacaagattg acaataactg aaaattacgt tggtaataat ggcgtattgc atctcagaac
     4261 tgaattgggt gatgataatt cagctacgga taaagtcgtt attaatggaa atacctctgg
     4321 aacaacccga gttaaagtta ccaatgctgg cggcagcggg gcttacacgt taaatgggat
     4381 agagattatc agcgttgagg gggaatcaaa tggagaattt attaaggatt cgaggatttt
     4441 cgccggtgcc tacgaatatt cattaacccg aggtaatacc gaagcgacca ataaaaactg
     4501 gtatctgact aacttccagg caacgagcgg cggtgaaaca aactccggag gaagttcagc
     4561 gcctactgtt gcgcctaccc ccgtcctgcg ccctgaagct ggaagttacg tcgccaacct
     4621 ggcagccgct aacactcttt ttgttatgcg tctgaacgat cgtgcaggtg aaacgcgtta
     4681 catcgatcct gtaacttaac aggagcgttc aagccggctt tggctacgtc aaattggcgg
     4741 gcataatgcc tggcgtgaca gcaacggaca gttgagaacg acctcgcatc gctacgtctc
     4801 gcagttaggg gcggatctgt taaccggtgg ttttaccgac agtgacagtt ggcgtttggg
     4861 agtgttggct ggttatgccc gcgactacaa ctcaactcat tccagcgtgt cggattatcg
     4921 ttcgaaaggg agtgtcagag gctatagcgc agggctgtat gccacctggt ttgccgatga
     4981 catcagtaaa aaaggcgcat acattgacac ctgggcgtaa tatagctggt ttaaaaactc
     5041 ggtgaaaggg gatgaattag cctatgaatc ctatagcgcg aaaggtgcaa ccgtctcgct
     5101 ggaagcgggt tacggttttg ccctgaataa atcctttggt ctggaagcgg cgaaatatac
     5161 gtggctcttc cagccacagg ctatctggat gggcgtcgat cataatgcgc acacggaagc
     5221 caatggctca cgtattgaga atgacgcaaa taacaacatc cagacccgac tcggcttccg
     5281 cacctttatt cgtactcagg agaaaaacag cggtccgcac ggtgacgact ttgaaccttt
     5341 tgttgaaatg aactggatcc ataacagtaa agattttgct gtctcaatga acggtgtgaa
     5401 agttgaacaa gacggggcgc gtaatctggg ggaaattaaa cttggcgtaa atggcaatct
     5461 gaatccggcg gccagcgtct ggggtaatgt gggcgtgcag ctgggggata atggctacaa
     5521 tgacaccgca atgatggtgg gcctgaaata taagttctga tcccgccatt agctaaaaaa
     5581 ccgcgtcgaa ttcatcgacg cggtaacata tgaaatatta ttttttgccg atagcacgca
     5641 tggtgtcatc aattgccgtg atcaacagca tttgcgggtc tttagcgcaa acctgattca
     5701 gtttttctac cactttggcg cttagttccg gagattgctc aatttttaaa tcacggctgg
     5761 caacgctggc attacccatt accgcaacaa tttctgcaac ctgtgcgctg tcagtttttg
     5821 ccatttcgtt ggcttctgcg caagtaatat aggtttctga cggcaaaccg tttttaatat
     5881 tgtagtcctg cgcccaggtc attggtgcga aaacaaacag acccgccagt aaagcaaatt
     5941 ttttcatcat cattccttat ttcattttac ccagaattgc accacccgta ccgccaatca
     6001 cggcaccttt aatcgcccct tcgaggccat tgccggtcag aacgccagtg acagcaccaa
     6061 cggcggcacc cacttttgca cctttacgcg catttttacc gtcgcggcct ttttctgtta
     6121 ctgcaccaac accagcgcca acagctgcac ctttcagtac gccattaaca ccattgccag
     6181 taagtaaacc aacgcctgcg cctagcaatg cacctttcgt ggtgcggttc atatccgcca
     6241 tcgctggcgt ggagcagaac aatgctgaga taagcccgaa ggcaagtatt tttttcttca
     6301 acttagatgt ccggtattaa gtaagttgca cacacaataa tttcgtcttc aattaagatc
     6361 tgcttaacta aagaacgctc gctattattc agataattca aaatgagcgt gactgtgatg
     6421 ataggaatta tgttttttac gggaataaga ataatctcaa atgataaata actcattgat
     6481 tgacaatatt tttattcaag aagtgtcatt gagtgttaat acaatgttgt aaaggtaaga
     6541 taatctgatt tatgaatatt attgtgtgat ttttatgtga gcagaaaata ttcatcagca
     6601 atgattacat tagtcatttt attttgtcga cggcctcatt gtcgaaaaat aagcgtacaa
     6661 cagtattatc agaaaagagt gattttttat ccaactacac ttcagcgcac tgcgtttaaa
     6721 aaatgcctct ttcttatgcg ggatatcatt atttcatcat gatgtctttg gtgagcggtg
     6781 aacacaatac acttgcgctg cctattcagg agcaatcccc tggtctggtg cctgcgggat
     6841 gatgttgcag cagagctaag gtcacttaga cgctactatc atttatccaa tggcatgggg
     6901 tcgaaatcag tcgatacccg cagtatatat cgtgaacttg gtgcaacgct gagttacaac
     6961 atgcgtctgg ggaacggcat ggaagttgag ccgtggctga aggcggctgt gcgcaaagaa
     7021 tttgtcgatg ataaccgggt gaaagtgaat aatgacggta attttgtcaa tgatttgtcg
     7081 ggcagacgtg gaatatatca ggcaggtatt aaagcctcat tcagcagttc gttaagcggg
     7141 catctcgggg tggggtatag ccatggtgcc ggtgtggaat ccccatggaa cgcggtggct
     7201 ggtgcgaact ggtcgttctg accatcaacg attaaactgc gcttcgcgca gttttcgttt
     7261 accgggtgct aaaagggaaa attctgcggc aaaaaaagcc cgccagttac ggcgggaaac
     7321 ctcatcctat gggagaacaa tgaataatga aattgcgggg ttatcatctc ccagtatacc
     7381 catactaaca ataaggttat ttactcagcc aggtataaac aatttgttct gtgtgggggg
     7441 aacggcatta agatgtaaag ggggggaagc aatgagtagc atcagcaaga atactcgccg
     7501 ctcttatcaa acgtggatga gggggatgaa aaactcaagg cagagatgac tctgccttga
     7561 agataaatgc gcttttacag cgggcttatt tcagctcttc tgcttccggt aaggtcacgt
     7621 tcagctcaag aatagaaata tcgccatctt tttgctcaag ctgtacggtt accatctcag
     7681 gatcaatttg tacgtattta caaatgacct caagaatatc tttacgcaac tgcggcagat
     7741 aatgcggttc tgcatcactg cgacggcgtt cagcaacaat aatctgcagc cgttcttttg
     7801 caatgttggc cgtgtttttc ttccgcgata gaaagaaatc gagtaatgcc ataacttatc
     7861 ctccgaacaa gcgtttgagg aagcctttct tctcttcttc aatgaagcgg aaaggacgtt
     7921 cttctcccaa cagacgttct acggtatctg cgtaggcttt acccgcatcg gcgttaatgt
     7981 cgagaatgac cggttcaccc tggttagagg cgcgcaatac tgattgatcc tcagggatca
     8041 cgccgacgag tttgatgcgc aggatctcca gcacatcttc catgctcagc atgtcacctc
     8101 tgcttacgcg gcctgggtta tagcgcgtta acagcaggtg ctctttaata ggctcttcgc
     8161 cattttctgc gcggcgtgat ttcgacgcca gaatgcctaa aatacggtca gagtcgcgta
     8221 ctgaggagac ttccgggttg gtggtaataa tggcttcgtc tgcaaaatag agtgccatta
     8281 gcgcaccggt ctcaatccct gccggggagt cgcaaacaat aaattcaaag tccatcgctt
     8341 tcagatcatc aagaactttg gcgacccctt cacgggtgag ggcatcttta tcgcgagttt
     8401 gcgatgccgg aagaatatag agattttcag tacgcttatc tttaattaac gcctgattta
     8461 gcgttgcatc gccctgaatg acgttgacga aatcgtaaac gacccgacgt tcacaaccca
     8521 taatcaggtc gagattacgc aggccgatat caaaatctat cacgacagtt ttctttccct
     8581 tctgggccaa accagtggcg atggccgcgc tggaggttgt cttaccaaca ccccctttgc
     8641 ccgaagtaac aacaataatg cgtgccatag aaattccttg ttaaaaaggg atcaatttaa
     8701 cggttgaacg gtcaaagcgt tttcgactaa ctgcagtcgc gccgctttgc cataaaattc
     8761 tgctgggatt tgatcactca gccagtattc acctgcgatg gacaccagtt ccgccatcag
     8821 gttcgtacaa aatatttgcg tttcccggtc accacttgcc cctgccagcg cacgaccgcg
     8881 catcatgcca tagacgtgaa tattaccatc ggcaatcaat tcggccccag cgctaacgtg
     8941 gcttgtaaca atcagatcac attgtggagc ataaatacgc tgaccggaac gcaccggggt
     9001 atctattaaa cgcgtttttg tgaccggcgt tgtattttgc gctggagcct gcggtgcggg
     9061 agctggacgc ggcgcttttt cctttccttc cgtcaggata ggcagcccca tcttttcaat
     9121 ttcggcttta agttgcgcat ctttgcagcc acttacgcca ataacccgca aaccggttgc
     9181 cgaaaccgcc ttatgcatcg ctgaccagtt taccgggtct tccagtgcac tgacgttgag
     9241 tacaacgggg gcatgtttta aaaatgcggg ggcctgagcg attttgtctt ccagcgcctg
     9301 atggataacc ttaggttctg cctcatgcag atgaaccaca gataaagtga agctactgcc
     9361 tttaagctcg attggcgtgt ttgacatcct ggccttactc aattagctat taatcatcgc
     9421 cagcgcgcga tgatgttccg aagactataa ggcatgttat agtctggatt atattgaggc
     9481 aagtcaccct cccatttatt cagagtaaaa gtctattctg tgataaatgg cgctgattca
     9541 tagcttaaaa aatacccttg tcaatcaacc cattgccgtc gtacttttga ttgttcttat
     9601 ttacgcttcc tttttccgca ccctaatcgt gacagcatct tcagacctgt ttaaatatcc
     9661 agtattgagg cgagtgtatt ttttcaccac gccaggcttt cttaactttc catttttgca
     9721 gcatcattaa agaacagata cccgttatct taatatccgc cacagaattg ctctatcata
     9781 gaaaccgcag aagacgtctg atacttctgt caggtcatcg taactcgtcc aggtcagcgg
     9841 ttttgattac ggattgatga tgaaatgagc ataagtgttg tacggttgga ttatcaggat
     9901 gtgctatgaa tatgatgcgt attttttata tcggattgtc aggtgtgggg atgatgttct
     9961 catcaatggc gtctggacat gacgctggcg gacttcaatc tccggcgtgc ggcgttgttt
    10021 gcgacccgta tatttgtgtt aactcagatg gcatttcccc agagttaaca aggaaatatc
    10081 tcggcgaaaa agccgctgaa aacttacaat cattacaagg ctacgatccc agcgaattta
    10141 cattcgctaa cggtgtattt tgcgatgtta aagaaaaatt atgtcgtgat gatcgttatt
    10201 ttggtgtgaa tggtaagcgg agtggaaaaa tcaatcaaac caccacaaag atgttattta
    10261 tgtgtcgtga gtgatgtctt gtttacatta tgttctattc gtcattaacc tcttgagcga
    10321 cagagaggag tggggcgtta acgtttatga cggggagagt ccccgtcggt tgatatttac
    10381 ttaatttgaa tatcgacgtt atatttttta gttttgtttt tacgggcgtc gtttcgggtt
    10441 tgcagcactc gcaactcata tttgccagaa gcagggagtg agtattgtcc gtgactatcg
    10501 agttctgggg aatatctgga taaatcaaca gaatcatcga ttcctggccc gaagaggtaa
    10561 gtatcagcgc cttcattaga gatactcaca tgcactttct gacctttttt ggcatagaag
    10621 gtatatgtat cgtaatcgta tccctttatt tcgccggagt actgagcgct gctatggcct
    10681 tttctgaact caacattgac attcttaccc gcagcaaagc tcgtggaggt tagcagtgca
    10741 agcaacaata cagccttact gatgccctta attttcattg gtagtctcct gttcagaatg
    10801 agtacgatga actgttataa tataacaatt catcacagga gaattccagt ggctttcctg
    10861 cgttcggtta aatcccggcg cggcattttt gccgggggga ttacttataa agggggcatg
    10921 gactgaacaa accgtaggaa tctacagctg gctggcttgt ggcgcgggtt tcattgatgt
    10981 tagcttatgc caaaacctgg catcttaaag agtaaaagta tgttttgtgt gatttatcga
    11041 agcagcaagc gtgatcagac ctatttatat gtcgaaaaaa aagacgattt ttcgcgtgtt
    11101 cctgaggaac tgatgaaagg ttttggtcag cctcagttag cgatgattct tccgctggat
    11161 gggcggaaga aactggtcaa tgccgatatt gaaaaggtta aacaggcatt aaccgagcaa
    11221 ggttactatt tgcagttacc gccaccaccc gaagatttgc tgaagcaaca tctttccgtc
    11281 atggggcaga aaacagacga cacgaacaaa taaccgatat ccggcggtgg cattatcttt
    11341 gtcggcgcgg gttttcatat ccacgataag gtgaggggaa cgttatgtat caacatcaca
    11401 actggcaagg tgcgctgctg gattatccgg tgagtaaagt agtctgtgtt ggtagtaact
    11461 atgccaaaca tattaaagag atgggcagcg cagtgcccga agagccagtg ctgtttatta
    11521 aaccagaaac ggcactgtgc gatctgcggc agccattggc gatcccatcc gatttcggtt
    11581 cagttcatca tgaagtcgaa ctggcggtat tgattggcgc gacgctgcgt caggctacgg
    11641 aagagcatgt ccgtaaagcc attgccggtt acggcgtggc gctcgatctg acattgcgtg
    11701 atgttcaggg aaaaatgaag aaagccgggc agccgtggga aaaggccaaa gcgtttgata
    11761 actcttgtcc gctttccggg tttattcccg cggcggaatt caccggcgat ccgcaaaata
    11821 caacgctggg cctgagcgta aacggcgaac aacgccagca aggtacgact gcggacatga
    11881 tccataaaat cgttccgctg atcgcttata tgagcaagtt ctttaccctc aaggccggtg
    11941 acgttgtgct gacaggcacg cctgatggcg tcggcccgtt gcaaagtggt gatgagctga
    12001 cagtcacttt cgatgggcat tctttgacaa ctcgcgtttt gtaatacttt ttgccgcctg
    12061 aaagcggcgg caaaacttgc atcgctgtgc cagactggtt ataaggtgcg ttttaacgta
    12121 atggcggaac acctgatgag cgatgtacct ttctggcaaa gtaaaaccct ggacgaaatg
    12181 agcgatgcgg aatgggagtc gttgtgtgat ggttgcggtc agtgttgcct gcataagctg
    12241 atggatgaag acaccgacga aatctacttc actaacgtcg cctgtcgcca gctcaatatt
    12301 aaaacctgtc aatgtcggaa ctacgaacgt cgtttcgagt ttgaacctga ctgcattaaa
    12361 ttaacccgtg aaaatctgcc aacattcgaa tggctgccaa tgacctgcgc ttatcgcttg
    12421 ctggcggaag gtaaagattt acctgcgtgg catccgctgc ttactggttc gaaagcggca
    12481 atgcatggtg aacgtatctc tgtgcgtcat atcgcagtga aagaatcaga agtgattgac
    12541 tggcaggatc atatcttaaa taaacctgac tgggcgcagt gataaattta atagatgttg
    12601 atgggggctt tcttaatata cgtctggata ctcgccattt cgtagccctg tttttgaaag
    12661 cgctctgtca taatgagagt tacccgatac cttattatca acaggtaact ctcaatcttt
    12721 aaaaaacggc tgtgttttta aatgtgataa accttatacc ttgagtacat ggagagaata
    12781 atcgcttatc agacttcagg tacctcaaag agtgtctttt taccgtgtct tttctgatac
    12841 tcattacagg tgttaatcat ttttttggcc gcttctttta gcaaagaaag cattaaatca
    12901 tcataatcaa catagaatct ggttgtttca gtttccgttt ttatctcccc gatggcggct
    12961 atttcggtgg ttaatttcaa tttggcggca tcgatatctt tattcgcttg tttaaccgtg
    13021 ttagacaggg tggtaaagaa actctgcaca gattttaact tgttcttcaa ttctggaatc
    13081 agtttccctt caactacgcc cgctgcaaca gaatacagtt tcttgatgcc gtcatccagt
    13141 accttaatga gaatgccttt ctgggcgaat gcttctattc tattttttag ccgggcgata
    13201 tttttcattt ctgctggatg agcgtcgtcg ccagaaggcc acgtgagcac aggataagag
    13261 aacgaaaaat cagcagtcta tgcagcgaca aatattgata gcctgaatca gtattgatct
    13321 gctggcaaga acagactact gtatataaaa acagtataac ttcaggcaga ttattatgtt
    13381 gtttatcaag cctgcggatc tccgcgaaat tgtgactttt ccgctattta gcgatcttgt
    13441 tcagtgtggc tttccttcac cggcagcaga ttacgttgaa cagcgcatcg atctgaatca
    13501 actgttgatc cagcatccca gcgcgactta cttcgtcaaa gcaagtggtg attctatgat
    13561 tgatggtgga attagtgacg gtgatttact gattgtcgat agcgctatta ccgccagcca
    13621 tggtgatatt gtcatcgctg ctgttgacgg cgagtttacg gtgaaaaaat tgcaactacg
    13681 cccgacagtg cagcttattc ccatgaacag tgcgtactcg cccattacca tcagtagcga
    13741 agatacgctg gatgtctttg gtgtggtgat ccacgtcgtt aaggcgatgc gctgatgttt
    13801 gccctctgtg atgtaaacgc gttttatgcc agctgtgaga cggtgtttcg ccctgattta
    13861 tggggtaaac cggtggttgt gctatcgaat aatgacggtt gcgttatcgc ccgaaacgct
    13921 gaggcaaagg cgctgggcgt taaaatgggc gatccctggt tcaaacaaaa agatctgttt
    13981 cgtcgctgtg gcgtggtttg ctttagcagc aattatgagc tttacgcaga catgagtaat
    14041 cgggtgatgt cgacgctgga agaactatcg ccccgcgtcg agatttacag tattgatgag
    14101 gcattctgcg atctgaccgg tgtgcataat tgtcgcgatc tgactgattt tggcagagaa
    14161 attcgcgcaa cggtgctaca acgtacccat cttactgttg gtgtggggat cgcccagacc
    14221 aaaacgctgg ctaagcttgc caatcatgcg gcaaaaaaat ggcagcggca gacgggcggg
    14281 gtggtggatt tatcaaatct ggaacgccag cgtaaattaa tgtctgctct tcccgtggat
    14341 gaagtctggg ggattggacg gcggatcagc aaaaaactgg acgcgatggg gatcaaaacc
    14401 gttctcgatt tggcggattc agatatccgg tttatccgta aacattttaa tgtcgtgctc
    14461 gaaagaacgg tgcgtgaact gcgcggtgaa ccctgtttgc aactggaaga gtttgcaccg
    14521 acgaagcagg aaattatctg ttcccgctcg tttggtgaac gcatcacgga ttatccgtcg
    14581 atgcggcagg ccatttgtag ttacgctgcc cgggcggcgg aaaaacttcg cagcgagcat
    14641 caatattgtc ggtttatctc cacgtttatt aagacgtcac catttgcgtt caatgaacct
    14701 tattacggca atagcgcatc ggtaaaactg ctgacgccca ctcaggacag cagggatatc
    14761 attaacgccg ctacgcgatc tctggatgcc atctggcaag cgggccatcg ctaccaaaaa
    14821 gcgggcgtga tgctggggga tttcttcagt cagggagtcg cgcagctcaa tttattcgat
    14881 gacaacgcac cgcgccccgg gagtgagcaa ttgatggcgg taatggatac gctgaatgct
    14941 aaagagggca gaggaacact ctattttgcc gggcagggga tccagcaaca atggcagatg
    15001 aagcgtgcca tgctttcacc atgttataca acgcgaagtt ctgatttact gcgggtcaaa
    15061 taaacatagc ggcaggaaaa agcgctcccg caggagcgcc gaatggatta gcgaccgaac
    15121 aggtcacgtt ttttcgcttt aaacggctgg gaaatcacca ccagcaccgc gacaatcagg
    15181 taagcgataa aaataccgag cagccactgc ggcatttcca gacctaaaaa ttcccactga
    15241 cgctcggcgc aatcgccaga ggcgacaaac acttgcggca cccatttatc cagcggcagc
    15301 cattccggga aacgagccat aaaatcacag gtcgcaaacg gcgaaggata gagctgaagc
    15361 atggtgtgct cgtaagttaa ctgcacaccg cggaacgcac tatacaacca gataaccatc
    15421 gctacataac gcagcggagt tttcggggcg atcgcgccaa tcagcgcagc acccagaacg
    15481 ccgaataatg cgcagcgttc ataaatacag agcacgcaag gtttcagtaa catcacatgc
    15541 tggaaccaca gtgccgtcag ttccagtgct agagcggtaa acgccatcaa cagccacgcg
    15601 ccccggcctt gtgaacattg gttcaaaaat cgcaacataa tcatttccct gcaatatgca
    15661 tagagtgcgc agtttaaacc aattcattcg ctgcgccacc aggaggcagc aaaaatagga
    15721 cgcaattgga caattatcgg gcgaaaaggc aaaccgggca tttatatgcc cggtaagttg
    15781 ttatcaaagc gttgctatcc agcccatttg cataaaccat tcggttacag gggcaagcgt
    15841 aaactcgacg cagagcaagc cgacgagtgt caggacgagg gtgtaaggca gggccatcca
    15901 caccatgcgg ccataagaga ggcgaatcaa tggcgcgagt gcagaggtca gcaggaacag
    15961 gaacgcagcc tgaccgttcg gcgtagcgac ggagggcaga ttggtaccgg tattaatggc
    16021 gaccgccagc agctcgtatt gcttcaacgt gatagcgcca ctttccattg ccgctttcgc
    16081 ttcgttgata taaatcgtcc ccacgaagac gttatccgaa atggatgaca gcagaccgtt
    16141 gaaaatatag aacagcgaca gctgagcatg ttccgatgcc tgcaacacaa actgaataat
    16201 cggcgaaaac agttgttggt cgataatcac cgcgactacc gagaaaaaca ccgtcaacag
    16261 tgcggtgaat ggcagagact cggtgaaggc tttaccgata gcatgttcat cggtgacacc
    16321 ggtcaatgat gtcgccagaa taatgaccga caaaccaatc aagccaactt ccgccagatg
    16381 caacgccagc gcagtcacca gccagacgcc aataatcgcc tggacaatca gacggatttt
    16441 atcctgacgg gtacgctggt ggcggctttg atcgtcaaac tgttgcagta cttcgcggac
    16501 tttctccggc agcgtttcac catagccaaa ccaacgcagc ttctctacca gcaggcaggt
    16561 taacaggcca caaatcagaa ccggaacggt cactggcgac atgcgcagga agaaatcgcc
    16621 aaaatgccag ccagccgctt tagcgatgat caggttctgt ggttcgccca ccatggtcat
    16681 cacgccgcct aatgcggtgc cgacaccggc atgcatcatc aggctacgca gaaagccacg
    16741 gaactgttcc agaaccactt tgtaatgctt gtcgatatga ctatcgtctt gcaggtcggt
    16801 atcttcggta cgggaagagg ctacgcggtg ataaatacca taaaaaccga ctgcaacgct
    16861 gatcaccacc gccacgacgg ttaaggcatc gaggaacgcg gagaggaacg ccgccgccac
    16921 gcaaaaagag agcgacagca gcattttgga gcgaatgctt aacagcaaac gggtaaatat
    16981 gaacagcaat agctgtttca taaaatagat acccgccacc ataaacatca gtaacagcaa
    17041 gacttcaaga tttgccgcca cctcttcacg gacgtgttcc gcgctggtca tgccgatgaa
    17101 taccgcttcg atagccaaca gaccaccggg gagcagcggg tagcatttca gggccatcgc
    17161 cagagtgaaa ataaattccg ctaccagcaa ccagccagcg acgaaagggc tgatgaggaa
    17221 aattaacgga tttacgatta agaaaatgat gagggcgagt ttgtaccagt cgggggactg
    17281 gcccaaaaaa ttgcgccata gtgcgcggcc ccaggagatc tccatgatgg tttcccttac
    17341 cttagaaata atcaatgatg tttttatgtt taaacgcaaa gcttaacggt cagacaggag
    17401 tgaggcaagt cttgatagtc aaggggaaag agatgcagaa aatgaagcct tgatcccttt
    17461 tttcttcttt ttgtcttcta tcagcatagt tagccctctg gtatgatgag tccaactttg
    17521 ttttgctgtg ttatggaaat ctcactatgg tcattaaggc gcaaagcccg gcgggtttcg
    17581 cggaagagta cattattgaa agtatctgga ataaccgctt ccctcccggg actattttgc
    17641 ccgcagaacg tgaactttca gaattaattg gcgtaacgcg tactacgtta cgtgaagtgt
    17701 tacaacgtct ggcacgagat ggctggttga ccattcaaca cggcaagccg acgaaggtga
    17761 ataatttctg ggaaacctct ggtttaaata tccttgaaac actggcgcga ctggaccacg
    17821 aaagtgtgcc gcagcttatt gataatttgc tgtcggtgcg taccaatatt tcaactattt
    17881 ttattcgcac cgcgtttcgt caacatcccg ataaagcgca ggaagtgctg gcaaccgcta
    17941 atgaagtggc cgatcacgcc gacgcctttg ccgagctgga ttacaacatt ttccgcggcc
    18001 tggcgtttgc ttccggcaac ccgatttacg gtctgatcct taacgggatg aaagggctgt
    18061 atacgcgcat tggtcgtcac tatttcgcca atccggaagc gcgcagtctg gcgctgggct
    18121 tctatcacaa actgtcggcg ttgtgcagtg aaggcgcaca cgatcaggta tacgaaacag
    18181 tgcgtcgcta tgggcatgag agtggcgaga tttggcaccg gatgcagaaa aatctgccgg
    18241 gtgatttagc cattcaggga cgataatccc ttccgtttaa aaagcaaacc cctcaaacga
    18301 ggggtttttc gttgttttta cagatttccc attctcggcg ggcaacgttc cagcaactcg
    18361 acgctgccgt cttcgttttg ctgttccagc atcacatcaa atccccacag gcgatgcaca
    18421 tgcttcagca cttctttgcg cccccgatcc agcggtgcgc gattatgtgg aatgtaacgc
    18481 agcgtcagcg aacggtcgcc gcgcaaatcc acgttccaga tctgaatatt cggctccaga
    18541 ttacttaaat tatattgaga cgataaccgg ttacggatct cccgataacc ttcttcatta
    18601 tgaatagcgg aaatctccag ataattatgc cgatcgtcat ccagcacggt gaagaagcgg
    18661 aaatcacgca tcactttcgg tgacaggaac tggctgataa aactctcatc tttgaaatca
    18721 cgcatcgcga aatgcagcgt ttccagccag tcggaaccgg cgatatccgg gaaccagtat
    18781 ttgtcttctt ccgttggcga ctgacaaatc cgtttaatat cctggaacat ggcgaacccg
    18841 agggcatatg ggttgatgcc gctgtaccac gggctgttat agggcggttg gaagaccaca
    18901 ttggtgtggc tgtgcaaaaa ctccagcata aaacgttccg ttactttccc ttcatcatac
    18961 agatggttaa ggatggtgta gtgccagaag gtcgcccagc cttcgttcat cacctgagtc
    19021 tgtttttgcg gataaaaata ctggctcacc ttacgcacaa tacgcaggat ttcacgctgc
    19081 catgattcca gcagtggcgc atttttctcc ataaaataga gcaggttttc ttgtggttcg
    19141 gacggatagc ggcgcgcttc agcaaccgtt ttctcttcct cgcgcttcgg cagggtacgc
    19201 cagagcatat tgacctgact ttgcagatac tcttcgcgac tcttctgccg ggctttctct
    19261 tcttgcagcg agattttttg cgggcgtttg taccggtcca cgccgtagtt catcagcgcg
    19321 tggcacgagt ccagaagccg ttctacttca tcaacgccat aacgttcttc gcactcggta
    19381 atatatttac gggcaaaaat cagataatcg acaatcgaac tggcgtcggt ccagctacgg
    19441 aataagtaat tgtttttgaa gaaagagtta tgcccatagc aggcatgagc catcaccagc
    19501 gcttgcatgg taatggtgtt ctcttccatc aggtaagcga tacacgggtt agagttaatg
    19561 acgatttcat aggccagtcc ttgctgaccg tgcttataca gccgttcagt ctcgataaac
    19621 tttttaccga atgaccagtg cggatagtta attggcatgc cgacgctgga gtaggcatcc
    19681 atcatctgtt ctgaggttat cacttcaatc tggtgcgggt aggtatccag ccggtagagt
    19741 ttcgccaccc ggtctatctc tgccagataa acatccagca ggtcgaacgt ccagtcgggt
    19801 ccatcgctca aacgtgtggt gtccttattc atagaatcga tcgtcgccat acgcgcacct
    19861 cattgttgtc ggcgctctct gtgtggagca cctcatttca agcatagaac acctgttaaa
    19921 aaccgcgtcg ccggagaatt tttttctttg cgatttctta ttatcagagt gccactaatc
    19981 cgcttctgaa cggaatttta tgctggataa aaagggcgtt cagcaggaga tactaaagac
    20041 gccatattgc cgcagagtca gggagatgtg agccagctca ccataaaaaa gccgtatgtt
    20101 gaataatatt ttcaactgag ttatcaagat gtaattagat tattattctt ttactgtatc
    20161 taccgttatc ggagtggcta tgcgagttgt catactggga agtggtgtgg taggcgttgc
    20221 cagcgcctgg tacttaaatc aggcaggaca tgaggtcacc gtcattgatc gggagccggg
    20281 ggccgccctg gaaaccagtg ccgcgaatgc cgggcaaatc tcccccggat atgctgcacc
    20341 gtgggcggca ccaggtgtgc ctttaaaagc gattaaatgg atgttccagc gccatgcgcc
    20401 gctggcggtt cgtctcgacg gtacgcagtt ccagttgaaa tggatgtggc aaatgttacg
    20461 taactgcgac accagccact acatggaaaa caaagggcgg atggtacgtc tggcggaata
    20521 cagccgtgat tgcctgaaag cattgcgcgc cgaaaccaat attcagtatg aagggcgtca
    20581 gggtgggacg ctgcaactgt tccgtaccga acaacagtat gaaaacgcga cccgcgatat
    20641 cgccgtgctg gaagatgccg gcgtaccgta tcagctgctg gaatccagcc gcctggcgga
    20701 agtggagccc gcgctggcag aagtggcgca caaactgacg ggcggcctgc aactgcccaa
    20761 tgatgaaacc ggagactgcc agctgtttac ccagaatctg gcgcggatgg cggagcaggc
    20821 gggggttaaa ttccgcttta atacgcccgt tgaccaactg ctttgcgacg gcgagcaaat
    20881 ctacggcgtg aagtgtggtg atgaagtgat taaggccgat gcgtatgtga tggcgtttgg
    20941 ttcttactcg acggcgatgc tcaaaggcat tgttgatatt ccggtgtatc cgctgaaagg
    21001 ctactcgctg accattccaa ttgcgcagga agatggtgcg ccggtatcca ctattcttga
    21061 tgaaacctac aaaatcgcca ttacccgttt cgataaccgc attcgtgtcg gcggaatggc
    21121 ggaaattgtt ggttttaaca ccgagctgtt gcaaccgcgt cgtgaaacgc tggagatggt
    21181 ggttcgcgat ctctatccac gcggcggtca tgttgagcag gcgactttct ggactggcct
    21241 gcgcccgatg acgccagacg gcacgccggt tgtcgggcgt acacgcttta aaaatctgtg
    21301 gctgaatacc ggtcacggca cgctcggctg gacgatggct tgcggttccg gtcagttgtt
    21361 aagcgatctg ctctcggggc gcacgccagc gatcccgtat gaagatctaa gcgtagcgcg
    21421 ctacagccgt ggatttacgc catcacgccc gggccattta catggcgcac acagctaagg
    21481 aaacgagatg acccgtccga tacaggccag cctcgatctg caggcattaa aacagaatct
    21541 gtccattgtc cgccaggctg cgccgtacgc gcgcgtctgg tcggtggtaa aagcaaacgc
    21601 ttacgggcac ggtattgagc gtatctggag cgcgctcggg gccaccgatg gctttgcatt
    21661 gcttaatctg gaagaggcaa taacgttacg tgagcgcggc tggaaaggac cgatcctgat
    21721 gctggaagga tttttccatg ctcaggatct ggagatttat gaccagcacc gcctgaccac
    21781 ctgcgtacac agcaactggc agctcaaagc actgcaaaat gcgcggctaa aagcaccgtt
    21841 ggatatttat cttaaagtga acagtgggat gaatcggttg ggcttccagc ccgatcgcgt
    21901 gcttaccgtc tggcagcagt tgcgggcgat ggcgaatgtt ggcgaaatga ccctgatgtc
    21961 gcattttgcc gaggcggaac atcctgatgg aatttccagc gcgatggcgc gtattgagca
    22021 ggcggcggaa gggctggagt gtcggcgttc gttgtccaat tcggcggcga ctctgtggca
    22081 cccggaagcg cattttgact gggttcggcc tggcattatt ttgtatggcg cttcgccgtc
    22141 cggtcagtgg cgtgatatcg ccaataccgg attacgtccg gtgatgacgc taagcagtga
    22201 gattattggt gtccagacgc taaaagcggg cgagcgtgtg ggctacggcg gtcgctatac
    22261 tgcgcgcgat gaacagcgaa tcggcattgt cgccgcagga tacgccgacg gttatccgcg
    22321 ccatgcgcct accggtactc ctgttttagt ggacggcgtg cgcaccatga cggtggggac
    22381 cgtctcgatg gatatgctgg cggttgattt aacgccttgc ccgcaggcgg ggattggtac
    22441 gccggttgag ctgtggggca aggagatcaa aattgatgat gtcgccgccg ctgccggaac
    22501 ggtgggctat gagttgatgt gcgcgctggc gttacgtgtc ccggttgtga cagtgtaact
    22561 tgttatcgct ggatgcgacc agcgtcgtat ccagcgttac aaaacacatt aaacttaaga
    22621 ttcggcttct tcctccgcta cccgaacacc aatcttcaac acttcattat cctctttctc
    22681 ggccaccgtc cagatcattc cggcaaattc tacctgatca ccgacaaccg gtgccgcgcc
    22741 taacaactgc tggacaattt cacccagcgt ttgctgctta tcacgatatt ctcgcccgtc
    22801 ttccaggcca tatatcagcg ccacatcagc atatttggca ctggcttcga gaatgaagtc
    22861 accaaagaag cgttgatcta gcgcgaccgg tggcgactgg ctgaacagtt taccgagcgc
    22921 cgggagatcg cgttcccgac caattacaca caacacatcg ccttcgcgca gtcgggtgct
    22981 gccggtagga tgaagcaact ggttatcacg aaacagtgcc gcaatacgcg tctcttttgg
    23041 catatgcaga tcgcgcagtg ccgcgcccac gcaccattta tcggcactca actgataaac
    23101 aaactgctcc cacggatttt ccggatgaat atccagacca acgcgtgaca ccggacgtcc
    23161 cactggcgga acgaccactt tggctttttt cgccgcccac gagagtgatg tcccctgcaa
    23221 gagtagtgaa accagaacca caaagaaggc gacattaaag aacagtcgcg cattctccag
    23281 ccccgccatc atcgggaaca cggccaggat gattggcacc gcgccgcgta atcccaccca
    23341 gctgataaac acgcgctcac gcagattgaa gccacggaac ggtagcaatc cggcaaatac
    23401 cgaaagagga cgggcgaaga aaatcatcca tgcggacaaa atgagcgccg gaatggcaat
    23461 aggcagcaga tcgcttgggt taaccaacag ccccagcacc aggaacatgg cgatttgcgc
    23521 cagccaggcg aggccgtcga aattttgcag aatgccgtag cggttgcgaa tcggccgatt
    23581 acccagcaga aaaccgcaca gataaacagc cagaataccg ctgccttcca gcgccgtggt
    23641 taaagcaaaa attaaaatac cgccgcttaa tgccagcaat ggatataaac cggcgggcag
    23701 ggcgatgcga ttaatcattt gcagcagtaa ataaccaccg ccaagaccga tgacaatccc
    23761 gaggccaaat tgctgcaaaa tatcgaccac gaacatccag ctgacactgc tttcatgctg
    23821 ctggatcatc gcaatcaggg taatcgtcag aaagaccgcc attggatcat tactgccgga
    23881 ttcaatttcc agcgtcgagc caacacgttc gttaagcccc ttaccaccca gcagagaaaa
    23941 gaccgctgca gcatcggtgg agccgacgat agcgccgatt aataatcctt caatcaaatc
    24001 aagattaaac agccacgccg ccatcatgcc ggttaaacca gaggtgataa gcacgcccag
    24061 cgtcgccagc gacagtgccg gtcctaacgc cacacgaaag gaactggcct gagtgcgcat
    24121 cccgccgtcg agcaaaataa tcgccagcgc cagattactt accatgtagg cgaagggata
    24181 attatcaaac gggataccgc cgacgccatc aacccccgcc agcatgccga tcgccagaaa
    24241 aataaccaga atgggaatgc caagacggga agaaaatgaa ctaagtaaaa tactgctggt
    24301 gacaaggatg gatcctaaga tgaaaaggct aattattgtt gtggcatcca acggtcggtt
    24361 actcctgatt acgctgtctc ttatataaac cctaccatat tagcggcaga gacagcgttt
    24421 tacttagccc tgaagcgtga tttttttaca ttttaagaac aggatgaccg ctaatcgtca
    24481 actgggtgcc ttcctgggtg ttattcagaa tggcatgtgc gcccaacggc agtgtaaccg
    24541 tgcgttgttc atgaccaaaa tcgaggccgg taatgagtgg aatcgacagg cgggaacgca
    24601 aaaacgcgta cactgactcc aggttgtaac ctgcgtcata atcattaggc gtgctgccgc
    24661 taaagctacc gagaataatc gccttctgac gtggcaaaat tccagcatga tagagctgca
    24721 acagcatacg ttcgacgcgg aaagggtgct cgttaatatc ttccagcacc agaataccgt
    24781 tctcaatttt tggcatccac ggtgtaccaa tcagtgaaat cagcatcgcg agattgcctc
    24841 cccacagcgt gccttcggtc tgacatgtcg ggccttcccc ttgccattca atggtgaagg
    24901 tttcattgcg taacgccagc cagaaatggt gctcggtaaa ggcgttcagt tcatccgcgc
    24961 caaagtttgc taccagcatg gggccgctaa aggtgatgac attgccatgc gccagaagac
    25021 cgcactgaat ggcggtaaaa tcgctatgtc cgcaaatgag caacgggtca tgttgttggc
    25081 gagccaccag tgcctgccag tcaatatctg ccagtaaacg actggcaccg taaccgccgc
    25141 gtacagccag cacgatggtg ttgggggtag tcagtctggc aagagaattg agatcttcca
    25201 gacgctccgt ttccgtaccc gcaaaacgct cacaacgacg ggcaataacc tcgacgttat
    25261 tgacctgatg ccccgcgtcg gtaaggcgct ggataccacg cagcgcggcg tgctgtttaa
    25321 tgcagtaacc cgatggggca attaagtgaa acagagacat ggcaattcct tgctgacaac
    25381 agaaacgaaa tgtatatcat gccgcttagg tgtgccgttg tcacctcaac ggcgattcca
    25441 ggctataagg atagaagaag tgaaattgag atggtttgcc tttttgattg tgttattagc
    25501 gggttgttca tcgaagcatg actatacgaa cccgccgtgg aacgcgaaag ttccggtgca
    25561 acgtgcgatg cagtggatgc caataagcca gaaagccggt gcagcctggg gcgtcgatcc
    25621 acaattgatc acggcgatta tcgctatcga atcgggtggt aatccgaacg cggtgagtaa
    25681 atcgaatgcc attggtttga tgcagttaaa agcttcaacc tccggacgtg atgtttatcg
    25741 ccgtatgggc tggagtggtg agccgacgac cagcgagctg aaaaatccgg agcgtaatat
    25801 ttcaatgggg gcggcttacc tgaatattct ggaaaccggc ccgctggcag gcattgaaga
    25861 tccgaaggta ctgcaatatg cgctggtggt gtcatatgct aacggggcag gtgcgctgct
    25921 acggactttc tcgtcagatc ggaaaaaggc gatcagcaaa atcaacgatc tggatgctga
    25981 cgagttcctc gatcacgtag cgcgaaatca tcctgcgccg caggctccgc gctatatcta
    26041 caaacttgag caggcactgg acgcgatgta aatcagtcgc gcactttgtc cgctttctcc
    26101 cgggcttctc gctcgagaga gaaaataatc cgctgtaatt gccgctccac cgtcgggctg
    26161 acgttaagaa aacggaagct cagacgggga gtggtgatgg tttcattctt gccatcaatc
    26221 actttgcgct cgctgatgga gattaactgg gcgtcaaagt gaaaaacacc ccattgcccc
    26281 atgttgactt caatctgagc gaagcgcatg ccttcgtgca atccggcagg ctttgctgtt
    26341 tccagtaatg cgcccatgcc gcctaacgac aaatcataca ggcggaaacg taacgtactg
    26401 ttatccgcca gtttggtctg gcaaaaatat ggcggatgga gtggggcgga gatgcggaaa
    26461 tatcggcgtc gttgtacaaa ccacaaggtg ggaggcggta cggtaataaa tgccggaagc
    26521 tgcaagtatt cactctgctg tagttgttca acagtaaact cgactttcgc accctgggtt
    26581 tcggcggtaa tggtaatgtg ctgcgccttt aacacagcgt tgttatcttc ggcttgactg
    26641 ccgaaatcca gcaccagttt atccggggtt attgccagta ttttgctgat cagctgcccg
    26701 ccattccaac tgatacgcaa aggaattgcg gctttgtgca aatcgcgtaa cacgcccagg
    26761 acggctaacg gattttgttt caggaactgc tcatggtaat gactcacgcg gaaaaactcc
    26821 tgactgacaa actgtcttag gtttatcggt cacagttaac aaaacttaat acaaatgcgt
    26881 gaatattttt tacatgtcgt tcttaaatca gccgacatac gcccagcatc gatcccgccc
    26941 ttgcctatac ttagagcgtt gacgtaagca tttcttgcgt ccgattcatc gaaacgaggg
    27001 cctgaacatg ggaattattg cctggattat ttttggcctg atagccggca ttatcgccaa
    27061 gctaatcatg ccggggcgtg atggtggtgg atttttcctg acctgtattc ttgggatagt
    27121 cggtgcggtg gtcggcggct ggctggcgac catgtttggc attggcggct ccatcagcgg
    27181 ttttaatttg cacagcttcc tggtggcggt agtgggcgct atcctggtgc tgggcgtatt
    27241 ccgcctcctg cgaagagaat aagattttca taaggcggat agcgatacag atgccgctat
    27301 ccgctttcac atcagaacgt atactcgata cctgcccaga aattgcgacc ttcttcaata
    27361 aacccttcac tgtaggcata tttggtatca aacaggttat tgaccgacgc attgacgctg
    27421 aagccatgac ctaaggtgta atcggctcga atgtgggtca ccgcaaaacc ggcggctttt
    27481 tgtgaaccgt cactgttgct gtagctggag gaacgcgcct cttccgacag cgttacgctt
    27541 aacggctccc acggtttgag agtcatccat gcggtcattg tctgcgttgg cagatcggtt
    27601 atcttgccga tgtctttgcg tttggcatca gcgtggatca gggcgtagct caatcctaca
    27661 tccagtatat tgctgatttt ccccttaata ccggcatcca gaccgctgta atccaccgtg
    27721 ccgctgttct gattttgaat ggtatcggca tcgatattgt gcgagaggat ggcatcactc
    27781 acccggttat agtaaacgct gacctcaaac ccccagtcgt gcgtgaaggc accattccag
    27841 gttaaatcca ccccgcgcgc gcgttccggt ttgagctgcg ggttaacaat cgctatctgg
    27901 ttatacgcag gtttggacgt ggtatagcgt tctttcagcg tcggaaagcg tgtgcggtca
    27961 tagtacgaaa gcgccagcgt gtcttcattg gcaaagtggt atttccccat cacctgccag
    28021 ttaaaagctg actgattgtt gtcgtcataa tgggtgatgc ttccatcttt ctcatgtttt
    28081 ttcgcttcta cgctatcgcg ccagtcatag ctgattccag ccacgacatc gacattatcg
    28141 gcagcagccc attgatattc actggcgagc gaccaggtac gatcttcata gcgatcgtaa
    28201 gcggcgtgcg gcgcaccttt ttcccggtgt acgtcatctt tccagttaac ggcaaacgac
    28261 agcagatcgt tttcacgcac atcggctgcc agttgtaacc cggcaccgtc gctgtaatcg
    28321 gaataatggc tgtagctgcc ttttttattt ttcaaatcag ccagcgagtt gtacatcatc
    28381 agcgtatttt caaaggtatc gcgatacagc cgacttttca gggtaaaacg atcgtttagt
    28441 tgggtcgttc cctgataata aaaactttct ttgtcatact ctggccactg ccagtagcgt
    28501 gatttttgac cactatttcc actgtatggc ggattatctt tttcaccatc ctgcttaatg
    28561 taagtcaatg tgtattcatc gttttcgcgt ggtgtaaaac cgagcttcac aatgccgcgt
    28621 ttatcatcag ccgatgaatt aatcatcttg ccgtgtttgc ctgcaatatc attatttaca
    28681 ccatgcggca ggccgagaaa atcctgcttt agctggctac cgctgacttg caaatacccc
    28741 agatcgctgc tggcggcaaa tgaagcatgc atatcgtagg cattgtcctg gctacggctc
    28801 catccctggc gatatcccag atttgcttcc agaggttttg ttggcttctg ggtggtgata
    28861 ttaatggctc cgcccatctg attaggcccc tgaagcagcg acgaataccc tttggaaact
    28921 tcaactgccc ccaaattgtt ggtcagaatc cgcgccagat cgaggttgcc gtcataggga
    28981 acataaatgg gcacaccgtc gaaatagact ggcacctgac gactatcaaa gccacgaact
    29041 ttgacctgtt cttcgttgcg gctacctgac ttttgcagca ccacgccggg gacgacactt
    29101 aatgcctgag cgacattctg tttatccagg gccttcatgg tggggtgatc gagaacggtg
    29161 gtcgtcgtcg atgataccgg actggaccat acggtcagcg tgtcactctc ctgagccgct
    29221 aaggcctgct gggtaaaggc aagtgataat gctgtgcaga gataacgttt tttgagtctc
    29281 atagaataaa ttccgtttaa caaagaatta aatagaggct gcgggttgca gactgacttt
    29341 aatgtcagct ggtgtcgcgt aataaggcgc ggaggtaata aaaaggcgaa tgccgcagtc
    29401 gaggtaattt ttgagtgttg tcagattaat tccgccggtc agagccagcg tgcagtgagg
    29461 agccagcgac ggtgctattt gcgcaatttc agttgcctgt tgcggattaa atttgtcgag
    29521 ctgaagtacg tctggttgcg cgcgtaacgc ggcgattgcc tctttcggcg tgtcggcttc
    29581 aacaactatt ttcttctcgg gtgcgtggcg gcgtaactga ttgattgcgc ctgaccagtc
    29641 ctgattgtca tgaagaaaat gacggtggtt ggcaaacaat aatatggttt ccgcacatcc
    29701 ggcacgatga atcagtcctc cggcagccag aattgcctgc gaggccagta agcgggtgcc
    29761 cggaattgct tttcgggtgc aggcgatatt gccatcaggg taacgttcac gaagtaacgc
    29821 cagcatttga gcgagataat cagaaacacc gcaactccac tccagcacat tctggactgc
    29881 cttccatccc tgatgaagtg ctgcggcatt accctgcgca cggattagcc gctgacccgc
    29941 gttcgcttgt gaaccgtcgc tgactgcgtc atcaatagtt aatcccagcg tagttaacat
    30001 cttacacgcg acagaaattc cgctgacgca gccgccctga cggtgaaaaa actctatata
    30061 gccatgctgg tgtccaatat ttaatgctcg cgtggtcagg tcaccgccct ggatatcttc
    30121 cagaagtaac gcatcgattt gtgcctgaga aaggaaaatc atcgcagtgc ctcttgcggt
    30181 acgctatccc accagatgag cgcccagtcg cggctggcag gggcgagggc acgagcatct
    30241 tgttgttgat accagcgata taaccgctca cgctcatcgt cgttaagcgc gcccagactc
    30301 cagctgacgt tctgctcaaa acgttcccag gtgctgttat cctgttggca attttgtccg
    30361 cggataaaat caacgtgagc ataaattccc atctgataca ggacgttgag cgcaaagata
    30421 tagttgggta attcgaccac ttcccgacca gccgcccgct gaatggctgg agagacgaac
    30481 gaggtgctga ccagatgagt cgtatagacg cgaagccgtg cctgattatt cagtttgctc
    30541 attgcctggc gcatatcagc caccagagtg gagcgggaag ccacggcaat atcgcagcgt
    30601 ggcaggtcac tccagtcctc ttcccaggcg cgctggatcc agtggacatt gtctgccttt
    30661 agcgcggcgg cgcgacgggc ggcaacgttc agcatcccct gactgtaatc aacgccataa
    30721 atcgtcgtga gtttgtccgc cagcgccagc gatacggtac ccggtccgca gcccatatcg
    30781 aataaggttt gcgcgccttg caggtcaatt tttgaggtca gttgttgcag gtagctgtcc
    30841 gtcggcgagg cgcaattttc cgccattttt tccgcccgtt gatcccagtg atccggcgtt
    30901 ttttctgtgc gatgggccag cctgagttgc tgaaggtaaa ggtctgcaaa atcaatatcg
    30961 tcaatgagca tgatttttcc ttagtgctta attgcagaga ggtgatggtg aatctggtcg
    31021 gcagaaaccc ggtacagggc agccagtttt ttcgttgtta actgttcggt cggtaagcct
    31081 tgtgttactc gtccatctgg ctctacctgg ataatgctgt cggcaatggc gtttgcatgg
    31141 agcggatgat gagtcgacat taacattgtc atgccgttat ttttgagctg cgccagcgtg
    31201 tccagaagtt ggatctgatg accgaaatcg aggctagagg caggctcatc cagtaataac
    31261 aagcgcggtt gctggacgag tgcacgggcg attaaagcca gctgacgttc accgccgcta
    31321 agcgtattcc agcgtcgtgt cgccagatgc aaaatgccca gcttttccag ttgctccgtt
    31381 gctttcaggc gttcttgctt accgggaacg gaaaaagcgc ctatggtggg ggcctggccc
    31441 atcaacacca tatccagcac actaaaagca aaaatgccgt cgtgcgcctg cggcacccag
    31501 gcaatagcct gcgcgcgctg gcgatggctg agtgtctgca cagggaggct atccagtaag
    31561 atctcgcctt ttaataacgg tataacgcca agaatggagc gcatgagtgt ggttttgccg
    31621 caaccattgg ctcctaacag acaacaaatt gttccttgcg acagggtgaa ggacacgtcg
    31681 cgtagcaccg gatggcggga gtaacccaga gacgcatgac ggacggcaag caaagtcact
    31741 ggcgacctcc gcgtaacaac aaaaacagga aaaacggtgc gccaacaaaa gcggtcagaa
    31801 tgccaagtgg gatttctgtt gttccaatgc tgcgtgccag cgtatcggta agcaggagca
    31861 gaatcgcgcc agtacacatt gccatgggca acagctgctg atggttatga ccggtcaaca
    31921 agcgggcaat gtgcggaacg accaggccga cccaaccaat tatgcccgct attgctaccg
    31981 tgctggcggt gatgagtgtg gcacagacaa tcaatccaaa gcgcagacgt gtcacgttca
    32041 gtcccagcga acgggcttcg tcgtcggaaa gcgtgagtaa attcatgcgc cagcggagaa
    32101 aaaacagggg caaggagcct atgagaatga tcggtgcagc atagcagaga tcgcgtaagg
    32161 tcacggtgga cagccccccc agcaaccaga aggtaattga cggtaactgg gtataaggat
    32221 cggccagcgt ttttattagc gatatccccg ctccgcacaa cgttcctaac gcaatgccta
    32281 ccagcaccag ggttaagatc ggatcgtggc gggtcacccg acgcgtaatc aaccagacgc
    32341 ccgcgaccac catcagcccg ccgcaaaatg cgtacagctg gatgtaaaca atggataatc
    32401 cccacaggat tgccgcgcag gcaccaagtc cggcaccggc ggagacgcct aaaatatccg
    32461 gtgacactaa gggattacgg aacattccct gataggtggt tccggcccct gccagcgccg
    32521 cgcccagaaa cagcgcagca agaatacgcg gcacgcggat ttgccagaag acaatttgtt
    32581 cctgcacagt aacattttct tgacgaagaa tgagcgccag cagttgctgc atatcgagat
    32641 gataagcccc acttacggcg gcaaaagtga tactgatagc tgccagcagc aaaatacatc
    32701 cgtttacggt tctcattagc tcgccaccaa tttttgatag tcggcgtcac ttaatgggca
    32761 atgccagaac agttgggcgt aatgctgcat gtcactttta aactggcgat tgatggcggg
    32821 atccagccag gcgtgaagtc tgcgtaatcc cagaagacgg ttgattcccg gcggggcatc
    32881 cagccagcca aagggcaggc cacttaaaaa taagatgcgt tgttccgcaa ccgctttcac
    32941 gccctgccag agaggatcac gacgaataaa atctgcagta acggcctcct gaaccagaat
    33001 aatatccggc tgccagcgca ggagattttc catggaaacc tgtgtcagac cgtggcgatc
    33061 ggctatttgc gcgacgttgt gcaaacctaa taattccgcc gcctcggtat gcaacgaacc
    33121 ctgtaagccc gtttccagcc ctcgaggacc gcgcgcagca taaaagctga gattagcggc
    33181 gggtgaggtg gcgaatgcct gagcttcacc aacgaagcgg ctggcgagat tggcttgttc
    33241 tgcggcgcgg tgctcttcgc ctaacgtttt gccaagcgtt gttaactgtt ctgctgattg
    33301 ttccagtttc ccgttaagca ataaccaggg tatttgtgtc tgttcgctaa cctgccgtgc
    33361 ctgggagatc caggtttcat ccgtattgcc gcaatcaacg accaaatcgg gatgtaacgc
    33421 cataagccct tccagcgaga gtgtactggc gcgcccggcg agtcgtccca gcctggggaa
    33481 ctggcgaatg tgctctggca atggaattaa tgcctgacgg gcaaaatcaa aagaggaaaa
    33541 accgaccatt ttttctggcg cgacagccag caatagtaaa tcggcgggcg cgcctgcgct
    33601 gacgatgcgt gtaatgtttt gggcttcggg aagcgtcagt gaggcccaga atttgactga
    33661 ttgtgcaaag gacggcagat gatgcaggaa caatgtcgac gcaagaatct gtgtaaacct
    33721 ccgtctggtt agtgacataa gtaatatcct gataattaaa accgctatat acttacacat
    33781 atagcgaaaa agatcatgtt tgatgtcaaa atttgtctaa agagacggaa tgatttgctt
    33841 gttgcttaaa taacactttg ttccacaaga aaattgtcag ggagaatggg gagtggggga
    33901 aaatataaat agaggtgtaa tgattaacaa gttgataaaa taaaattaaa atcttctcta
    33961 ataaaagaaa ataaaggcat agaccgtaga aagagtggta tttggtattt cgtggtgaag
    34021 aaagagcaat gccaatttta tgcacgtaat ggcattgctc gatatacagt aaagttaagg
    34081 cgtaggttcc gcctcatttt gttttaccgg ctgtgttgtt gattcactga gtggacgcgt
    34141 cgccggaaca ttgtcgcacg gttgctcttt cgggcagatc aaatccagca ttttcagcgt
    34201 cacgccattg gtccagccaa agccatcctg taatggatat tcgccaccgc cgccccctgt
    34261 tccggtggtg ctgacatcat atttttccac cagctttttc tcccggtcat aggtgtgctg
    34321 aacattggtc aggaagtgcc agctaatgtc catcgccacc tctttttgcc cgtagttttg
    34381 taatccttct gtcgcgaccc actgcaacgg tgcccagccg tttggcgcat cccattgttg
    34441 cccacttttc accgacgtgg tgttcaggcc gcctggttgc agcagatgtg ttttcgtcgc
    34501 cgtcgccatt ttgctggcgc gatctttcgc tgccgcattg acgtacagcg ggaacagggc
    34561 ggccgcggtt aactgattgc gcactttatg acttttcagg tcgtaatcgg cataccagcc
    34621 ttgttgatcg ttccacaggt atttttcgat ccctttttga cgggcattcg ccagcgtttc
    34681 gtactggttt gccatcgcgt tatctccggc agctttgctg gcgcgggcga ggattttttc
    34741 cattttaaac atcaggctgt tcagatcgac cggtacgatg ctggtggtgc gtaaggtatt
    34801 taactgctgc ggattgtcca tccagcgcga gctgaaatcc cagccagacg cagcggcaga
    34861 gcgcaggtcg cggtaaattt cagtggcagg tcgattcgga ttgcttttgg cggtggcaat
    34921 atcttccacc catgactctg gtcgtggcgt atcgcgatcg tcccagtagc ggttgagaag
    34981 ggtaccatcc tgaagtttga caacgcgttt ttcctgttgt ccggcttgca ggttttcaac
    35041 accgtccatc cagtaagcat attctttttg catttgcggc aggtattgct tcaacgcggc
    35101 atcgccttca tgctgcgcca gtaactctac catcagggca aagaatggcg gttgtgagcg
    35161 gcttaaatag taactgcggt tgccgttggg aatatgcccg taagtgtcta tttcatgagc
    35221 aaaattggcc accatatccg cgactttatc ccagtgacca ctttcggcaa gccctaacat
    35281 ggtgaagtaa ctgtcccagt aatatacctc gcgaaaacgt ccgcccggca caacataagg
    35341 ttccggcagc ggtaacagag aatcccattt ttcggtattt tcggtagaac gcgttaatac
    35401 cggccaaagt ccgtcaatat gttcgcgtag tgattgcccc tctggcggaa catatttctc
    35461 gccctctttc ggcagggtga aattgacgtt aacgaaatgg cgtagatcaa atccgctctg
    35521 gttttgctgc atccgataat cagcaaggat catcagcgga tcgctgttcg gcacggcatc
    35581 ggcaaaggtt ttttggtccg gaaaaagttt ggcgttttgc acatcattaa acagcggccc
    35641 taataaaata tcaggcggct gtggcgttac cggtgtttct tctgcctgca ccgatagcgc
    35701 agcgaaacac aaaaagatgc aggctggaat taacgccatt tttagcgggc gagaagctgc
    35761 gggggatttc atcaatcatt ctcctttggc gaaaccgaat aaaacggtat tcaagtatca
    35821 gaaaacctta gttcaggatc gcactagctg catgatcgaa atctcattct gcgaacggtc
    35881 aggcatttta gaaaagggaa ttaaagggat aaataacgtc gaaatagagt gatcaaataa
    35941 cgtctgtttg ttagccagcc agcggcgttg ggttcgcgat tagcgcagaa aaaatcatca
    36001 tgatttggta aaaaatataa caggtagtta aaaatcatcc gtgcagagta aatagccgga
    36061 tggcatcaga acgatgccat ccgaacggtg gtttaaccct gacggctgaa acgttgcgtt
    36121 ttaacgtcca gcgttagcgt ttcttctggt tgtatcgcat acagtttctc accctgctgg
    36181 caaatccagc caatccccag ttcacgggcg atcagggcgc tgtgggatag cggacttccg
    36241 gcgctaaggc agataccttt tacaaccgcc ggatccagtt gcagtattgt ggagggatag
    36301 atgttctccg ccagtagaat agttggcgag ttaaactgcg ggagttcttc tttcgtttgg
    36361 gtcaggtgga ccagggtgcg atgcagaaga tcgtccacat caatatagcg agcctgtaga
    36421 tattcatcat ccagttgctg gtattgctgg ctaagttctt taagaacttg ctgccaggca
    36481 tattctgccg tgcaatgttc atgctgaagg agttcgcttg ccgccgccag cagttccgga
    36541 tcatctaaca gtgtatggtg accagaaaag attgcggcaa tatcgtcaag cccgctggct
    36601 tctgctttcg ctgttaacgt catcagatct aataacgtga agtcaatggc ctggcgtaat
    36661 cgttcttgtt cttcatccac ggtcagggtt gattttgcct gtaccgtaca taaaaccggt
    36721 tgataataaa aggctttacc cgaaacaggc ggaacgggac gcagaatagg tggagcaact
    36781 tcctccgttt caccaaagtt atcttcagcc agctgacgga aagcgatcag tgcctcttca
    36841 gcttctggcc ctttcgcaat caggcgcagc gtatcgttat agcgaacttg tagtaacgca
    36901 atctggttaa tactctccgg tgtgacgcat ttgccgtttt tctccagcaa catatcggca
    36961 ttaaatgtcg ataaggtata aaccagccgg gaggccggac gtacatgcag gccgttacgg
    37021 ttttttatga ccaccgccag agaacgggct tcttcatcgt acgaaggaca tgtgtcagag
    37081 atttcagtgt tggaggaggg taaacccagt tgttcacgtt tggcttccag cgcatgcatg
    37141 gcgtcaaaga tcactttgtc gatctccgcc cccgaggccg cgctaaccgt tgctgccagt
    37201 gtaccttcga ccaacggcgc agcacacaaa cgtacttttg cggcgatctc gggagccagc
    37261 aattccagcg cagtttcagc actcaataat gcgcttccta tatccatcat gaccagcaca
    37321 tggtcggtat cggcaacaga ttcgatggcc tccatcactt tgacggcatc ggtaccaatg
    37381 ggattttgtg gatcgtcaat tcccgcggca atggcgattt tacaattatc actcattaac
    37441 atctgacggg ctaattcacc gacgccttct cccagtcggc tgctatgtga aactatgacc
    37501 aggtttacca tcaccaattc cttactcttt tgcggctaac gccaacattt gcatcataaa
    37561 catcaccgag gtcgcaccgg ggtcctggtg accaatactg cgttcaccga gataactggc
    37621 acggcctttg cgagcttgca tcgtaatcgt actttgtgca gcggattcgg cgacgctact
    37681 ggcagcttcg agcgccgccg gaatagagag attttgctcg ctggactgac gtaacgattc
    37741 caccaccggc acccacacat cacacatggt tttatcgcca ggttcggctt tcccgcgact
    37801 gattacgccg tccgcgccat cgcggaacat ctgataaagc tcttccagcg tcaggctttg
    37861 ccgtgcctgg gtcgcctgtg cggcgcggat aaagaaggta ccgaaaagcg gaccactggc
    37921 accaccgacg ctggaaagta gcgtcatacc ggtattcttg agaatgaaac cgatatcttt
    37981 atctgcgata gcagggagtt tttccaccac tttgctaaag cctcggttca tatttagccc
    38041 gtggtcagcg tcgccaattt cgcgatccag tccggtaaga tactcgctct cggtgctgaa
    38101 aatatcgcca caacgagtga gccagttaac aatttgagtt ctgctcagtg acattgctat
    38161 ctccttattt accccagtta agggccgggg tgtggaccgg ggcgtcccag agtgccagcg
    38221 tttcgtcatc aactttcagt aaggtgattg agaaaccggt catatccagt gaggtgcagt
    38281 acgcgccaat taaattacgc tcgatagtca atcccgcttg ctggcaacgt gtggtcaggc
    38341 ggttatagac gccgtacagc tcagaaagcg gagttgcgcc aagattgtta accagcgcaa
    38401 tcacccgatc gccagactgg agcggttgtt tagtttgtga ttcttcctgc caactgcctt
    38461 gttgataatc ccagaaacgc aaagtgcgat ggtatgagcc attttccagc agggtgtcga
    38521 acatttcatc gacggtttga tcaagggaag agaagggacg gcggtcaata cctggctcac
    38581 catgaatgcc gacgccaaac tccatctcat tgtccgccag ggtaaaagaa ggtttgcccg
    38641 cggcaggaac ggtacaggca ccgagagcga tacctattga gtggccttga ttattcaact
    38701 tacgccccag ttccgcacag gcgtccagtg agtcgccacg ctcggccgct gcgcctacga
    38761 gtttttcaat taatacggtg ttggcaacgc cgcgtcgccc ggcggtataa agactgtctt
    38821 ttaccgcaac gtcgtcatca atgaccacag tggtcacttt tacgccgcta tcgtgcagta
    38881 actcggtcgc tgtttcaaag ttaagaatat cgccggtgta atttttgata atcaacagta
    38941 caccttcgcc gccatcaatt tgcatggcgc attcaaagat tttatcgggc gtcggtgagg
    39001 tgaaaatttc gcccggacag gcccccgaaa gcatcccctg accgatataa ccacaatgca
    39061 tcggctcgtg tccgctgccg ccacccgaca gcagggcgac tttacctgca acgggggcat
    39121 cagatcgggt gacatacacc ggatcctggt gcagtgtcag agatggatgc gctttcgcta
    39181 gtcctgccag ttgttcgtcc agtacgtctt gcacatcatt gatcaatttt ttcattattt
    39241 tgctccagca attacggtag ggcatggatg atgttcaacg acacggcgac cactgactgc
    39301 cgatgaatcc attgtgcatc aggcaaggga aaagaaaatt agcccaaatt atgtttcata
    39361 gtgaaacata ttctttaatg aatgttccat attgaaactt ttacgtgtat taatacttaa
    39421 aattgcgagc cggaacacct tttgtcataa gggatgcggg atatgagtag cgcttttaac
    39481 aacgatggtc gggacatatc tcccttaatt gcaacctcct gggagcgatg cattaagctg
    39541 atgaaacggg atacatggaa cgtaccacac caggcccagg gcgtgacatt tgcgtctatt
    39601 tatcggcgta agaaagcgat gctgacgctc gggcaggctg cgctggaaga tgcctgggaa
    39661 tatatggcac cgcgagcgtg tgcgctgctt atcctcgatg aaaccgcctg cattctcagc
    39721 cgtaatggcg atccgcaaac cttgcagcaa ctaagtgcac tgggattcaa tgacggcacg
    39781 tattgcgccg agggaattat tggtacttgt gcgctatcgt tagcggctat ctctggtcag
    39841 gccgtgaaaa cgatggccga tcaacatttc aaacaggcac tctggaactg ggccttttgt
    39901 gcaacgccgt tgtttgacag caagggccga ttgacgggaa caatagcgct ggcgtgtccg
    39961 gttgagcaaa ctaccgcagc tgatttgccg ttgacgttgg caatcgcacg cgaggtcgga
    40021 aatttactgc tgacggacag tttgctcgct gaaactaacc gtcatttaaa tcaacttaat
    40081 gccctgttag aaagtatgga tgatggcgtg attagctggg acgagcaggg taatttgcaa
    40141 tttattaatg cccaggcggc gcgggtctta cgccttgacg cgacggcaag tcagggacgg
    40201 gcaatcactg aactcttaac gttacccgcc gtattgcaac aagcaataaa acaggcacat
    40261 ccgctcaaac acgtagaagc aacctttgaa agccagcacc agtttattga tgcggtgata
    40321 acccttaaac cgataataga aacgcaggga accagcttta ttttgttgct ccatcctgtg
    40381 gaacagatgc ggcagttaat gaccagtcaa ttaggaaaag tcagccatac tttcgctcat
    40441 atgccacaag aggatccgca aacccgccgt ttgattcatt ttggtcgcca ggcggcgcgc
    40501 agtagctttc ctgtcctgct ttgtggagaa gagggcgtgg gcaaggcact gctaagtcag
    40561 gcaattcata atgaaagtga gcgtgcagca gggccttata tcgccgtcaa ttgtgagtta
    40621 tatggtgatg cggcgctggc ggaagaattt attggcggcg atcgcacgga caatgaaaat
    40681 ggtcgtttga gtcgactgga actggcgcac ggcggcacgc tgtttcttga aaagattgaa
    40741 tatctggcgg tggagttaca gtctgctttg cttcaggtta tcaagcaggg tgttatcacg
    40801 cgactggatg cgcggcgttt aatacccatt gatgtcaaag tgattgccac aacgaccgcg
    40861 gacctggcaa tgctggtgga acaaaatcgt tttagtcgcc agctgtatta cgcgctgcat
    40921 gcatttgaaa ttaccatccc gcctctgcgt atgcggcgtg gcagcattcc ggcgctggtg
    40981 aataacaaat tacacagtct tgaaaaacgc ttctctacgc ggctgaaaat tgatgacgat
    41041 gccctcgctc gcctggtttc ctgtgcatgg cctggcaacg attttgaact ttacagcgtc
    41101 atcgagaatc ttgctctgag tagtgataac gggcgcattc gcgtcagtga tttacctgaa
    41161 catctcttta ccgagcaggc gacagatgat gtcagcgcca ctcgcctttc caccagtctg
    41221 tcatttgcgg aagttgaaaa agaggcaatt attaacgcag cccaggtcac aggcggtcgc
    41281 attcaggaaa tgtcggcttt acttggaatc ggccgcacta cgctgtggcg gaaaatgaag
    41341 caacatggca ttgatgcagg gcagtttaag cgccgtggat agcgggagag tatgtacttt
    41401 cccgctatga attgctaatt aatagcgacc atgactgccg tgatttggtc caccatttga
    41461 aggtggcatt ggcttaccgg agggcgcagg accacgaggg ccatcgctgt ggcggtgatg
    41521 atggtgggga acacaagcac ttaacaggct cgcaaagaca attgctaaca ggctgaaggt
    41581 aaatgttttt ttcatgacaa actcttgaac ggttaatgca ctgtgattgt tatcacataa
    41641 aaggcacatt gttaatattg ccaggagtct gattatgatt ttcgataact cagaattgaa
    41701 agaagccgcg tagcaggc
//
LOCUS       DAZGQL010000036        40201 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.036, whole genome shotgun sequence.
ACCESSION   DAZGQL010000036 DAZGQL010000000
VERSION     DAZGQL010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40201
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.036"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..830)
                     /gene="fdhD"
                     /locus_tag="ACHKIR_002186"
     CDS             complement(<1..830)
                     /gene="fdhD"
                     /locus_tag="ACHKIR_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HFO9989925.1"
                     /translation="MKKTQQKEIENVTNITGVRQIELWRHDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     "
     gene            1024..4074
                     /gene="fdnG"
                     /locus_tag="ACHKIR_002187"
     CDS             1024..4074
                     /gene="fdnG"
                     /locus_tag="ACHKIR_002187"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:1609..1611,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFO9989926.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSIGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKVYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPSSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            4087..4989
                     /gene="fdoH"
                     /locus_tag="ACHKIR_002188"
     CDS             4087..4989
                     /gene="fdoH"
                     /locus_tag="ACHKIR_002188"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HFO9989927.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            4986..5621
                     /gene="fdoI"
                     /locus_tag="ACHKIR_002189"
     CDS             4986..5621
                     /gene="fdoI"
                     /locus_tag="ACHKIR_002189"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFO9989928.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            5618..6547
                     /gene="fdhE"
                     /locus_tag="ACHKIR_002190"
     CDS             5618..6547
                     /gene="fdhE"
                     /locus_tag="ACHKIR_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HFO9989929.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTTRIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(6762..6980)
                     /gene="yiiE"
                     /locus_tag="ACHKIR_002191"
     CDS             complement(6762..6980)
                     /gene="yiiE"
                     /locus_tag="ACHKIR_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFO9989930.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQRGETVVVDDQCKEHKE"
     gene            complement(7585..7875)
                     /gene="nadS"
                     /locus_tag="ACHKIR_002192"
     CDS             complement(7585..7875)
                     /gene="nadS"
                     /locus_tag="ACHKIR_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="HFO9989931.1"
                     /translation="MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRAS
                     GLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALLRAIANDPQHVLQALNR"
     gene            complement(7876..8187)
                     /locus_tag="ACHKIR_002193"
     CDS             complement(7876..8187)
                     /locus_tag="ACHKIR_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO9989932.1"
                     /translation="MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGG
                     LRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIYRKGIKDDLSPQEKAMLRLLNTR
                     W"
     gene            8416..9324
                     /locus_tag="ACHKIR_002194"
     CDS             8416..9324
                     /locus_tag="ACHKIR_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO9989933.1"
                     /translation="MAQEKGIAELVEEFIAAGRPSSRQQSIAQRREGYIASAVLAGET
                     ETRVDIQTIELEAMTLRIVSPLNAPTLLPTIIYYYGGCFVSGGFATHDNQLRQLAYYG
                     QCRVIAVQYRLAPEHTFPAAHDDAQRGAELVWQHAERLGVDKQRITLAGDSAGGHLAL
                     VTALRLKRAGEWQPAQLILIYPMLDATAHFESYIRNGHDYIITRDTLLSGFEMYLPGI
                     ERRHPEASPLWRDDFNGLPPVHIITAEYDPLCDEGEALYHRMTGQGVQCTCQRYLGVI
                     HGFFQLGGISEAARSALRDVAWRAGR"
     gene            complement(9388..10329)
                     /gene="fabY"
                     /locus_tag="ACHKIR_002195"
                     /gene_synonym="yiiD"
     CDS             complement(9388..10329)
                     /gene="fabY"
                     /locus_tag="ACHKIR_002195"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HFO9989934.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(10374..10811)
                     /gene="dtd"
                     /locus_tag="ACHKIR_002196"
     CDS             complement(10374..10811)
                     /gene="dtd"
                     /locus_tag="ACHKIR_002196"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFO9989935.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDCA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(10808..11680)
                     /gene="yihY"
                     /locus_tag="ACHKIR_002197"
     CDS             complement(10808..11680)
                     /gene="yihY"
                     /locus_tag="ACHKIR_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HFO9989936.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(11674..12273)
                     /gene="yihX"
                     /locus_tag="ACHKIR_002198"
     CDS             complement(11674..12273)
                     /gene="yihX"
                     /locus_tag="ACHKIR_002198"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HFO9989937.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRVPLATLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPDD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(12414..13301)
                     /locus_tag="ACHKIR_002199"
     CDS             complement(12414..13301)
                     /locus_tag="ACHKIR_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFO9989938.1"
                     /translation="MKTERKNEHYLALQQAFDAPWPGPVGELVTLEKGNIHLQIYPHD
                     GARITSLKAFGSEVLRQWQPQRRAFQYGCFPMVPWAGRLGNATLNAGGQCYSLPANKP
                     PHALHGMACYSTWEIIDKTADSLTLRMPLASPWPWQGEVIQTFLLENDALVLQLEVHS
                     YADTFPASAGWHPWFAKKLTPQNTESLQVLFDADWQEEAGSDELPTGNRISPQVGPWD
                     DCFGFYDGVKVKLLWPGKLAMTMTSSANSLVVFDKQPDATCINPLTQAPNAINLTPEF
                     VTPDKPLVIETRWQFTPES"
     gene            complement(13291..14640)
                     /locus_tag="ACHKIR_002200"
     CDS             complement(13291..14640)
                     /locus_tag="ACHKIR_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9989939.1"
                     /translation="MSEKLPAPREGLSGKAMRRVVMGSFAGALMEWYDFFIFGTAAGL
                     VFAPLFYPDSDPFIGLIASFATFGVGFLTRPLGGIVFGHFGDKIGRKITLIWTLAIVG
                     CSTFLIGFIPTYQEIGIWAPLVLMVLRLIQGFGLGGEYGGAALMTIESAPESRRGFLG
                     SLPQTAASVGIMLATGIFALCNHFLTSEQFLSWGWRIPFWLSAVMLIVGLFIRLHTEE
                     TLDFQKQKTTNNKEKSVPPLIELFKKHPRNILLALGARLAESVSSNIINAFGIVYISS
                     QLALSRDIPLTGMLIASAIGIFSCPLVGWLSDRIGQKSLYLSGAGFCVLFAFPFFLLL
                     DSKSTLIIWCSMILGYNLGPTMMFAVQPTLFTRMFGTKVRYTGLSFAYQFSAILGGLS
                     PLIASSLLALGGGKPWYVALFLFAVSVLSFVCVWLIEPTDEQETASYRYIREQSHEN"
     gene            complement(14739..16172)
                     /locus_tag="ACHKIR_002201"
     CDS             complement(14739..16172)
                     /locus_tag="ACHKIR_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9989940.1"
                     /translation="MSQITSPATYSISRPQDVIDIVNKNSAINTSIGVIFIALGGILI
                     DAYQAAMVGFGNKYIAAQFGISPGLAATVNASVLIAALIGGLLANRVINRFGQKRAFI
                     IGMGLCTIGAAAVAIAPSIWWVLVCRVIMGFGLGIDFPLATNAVAELRGSTSKKTGTS
                     VNLWQMAWYVSTTVVYLVLLPLLLSGIAEEQLWRYGIFIGAIFAVIFMILRYFFIGES
                     AMWAARVGRYQEACDILGKRYGVQARVAASGTTEAKFSEKAENKYSGGYGILFNDRYR
                     KRTILGCVVATMQAWQYNAVGVYLPLTLAGIISGGLTGALTGSAVVNALCGVTGGMIG
                     SFILQRLGTRRQSMYGFAVVTLALLSLGALATTNPWLSLGLLGSIIFFHSAGPGGLGM
                     TIATLSYPPAIRPTGVGFARAIMRTGAIAGLIFWPMLWGALKTEAFYWLAIVPFLGFL
                     TCVLINWEPLGANVDAEDAEVLAELKK"
     gene            complement(16242..17252)
                     /locus_tag="ACHKIR_002202"
     CDS             complement(16242..17252)
                     /locus_tag="ACHKIR_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFO9989941.1"
                     /translation="MDKITQVLFSDIGKVTTQYVEVPHQQLKPHEVRIAPVFYGICGS
                     DLHVLKGGHPFAKPPVVPGHEIAARVTEVGSDVKNVQPGDHVVVDPIMACMECRACKA
                     GRFNLCEPPQVAGFRAPGFARSQHIVPARNCHVAPASLPLKVLAFAEPAACARHCVNR
                     MPKASLESVLVIGAGTIGLSIVQALRIMGAGKITVIEPDAAKRALALKLGAAEVWAPG
                     ELAADVRFTGAIDVVAAQATLNDACTRVYAGGTVVCMGVPSGPREIPLPMMQRFERDL
                     LNSGMYIPEDFDAVIEWLADGRFDTSELVTDLFAIEDAAAAFERAQQNDSIKVMLQFA
                     PE"
     gene            complement(17285..18172)
                     /locus_tag="ACHKIR_002203"
     CDS             complement(17285..18172)
                     /locus_tag="ACHKIR_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HFO9989942.1"
                     /translation="MSSHFTPFTLKDISRPGGGFAMLAVDQREAMRLMFAAAGQPKPI
                     ADSVLTDFKVAATRILSPYASAVLADKQFCLEQIVEQGAVANSCGLIVAADLFIPGNG
                     IPVDSVEIDMSVDPHKAREMGAKAMKLLVLWREDEPAEERLAMVDKFVRRCRSAGLVS
                     IIEPVVRPPRRGWDFDRESAIVAAAAELGGTEADLYKAEMPLGGKGDEKTLLAACQQL
                     NDQMKMPWVILSSGVDADIFGRAVSIAMKGGASGFLAGRAVWASVVGAQDPQTMLRDV
                     SVPRLQRLAEIVDEGIAQR"
     gene            complement(18197..19075)
                     /gene="yihU"
                     /locus_tag="ACHKIR_002204"
     CDS             complement(18197..19075)
                     /gene="yihU"
                     /locus_tag="ACHKIR_002204"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HFO9989943.1"
                     /translation="MATIAFLGLGQMGSPMANNLLQKGHSLQVFDVNTQAVDALVTQG
                     ATAAQTPAEAAASAEFIITMLPNGDIVRQVLLGEKGVCETVSSDALVIDMSTIHPLQT
                     DALIRELQEKGINMMDAPVGRTSVNAIDGTLLILAGGTDEQIARARPILMCMGNELVE
                     AGGPGMGIRVKLINNYMSIALNALSSEAAVLCESLGLNLDVAIKVMSGTAAGKGHFTT
                     TWPGKVLKGDLSPAFMVDLALKDLRIAVDVARETGAPLNMGIAAESYYAAASQDGKGR
                     QDWSALLNQVRQQAGL"
     gene            19248..20144
                     /locus_tag="ACHKIR_002205"
     CDS             19248..20144
                     /locus_tag="ACHKIR_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFO9989944.1"
                     /translation="MTRIACVGITVQDRIYSLPTLPEGGGKYQANHYLEIGGGPAATA
                     AVAIAKLGVEVDFIGRVGDDSCGNTLLAELEGWGVNTAFCRRYPNARSSQSAILVDQH
                     GERIIVNYPSPDLGTDAEWLEAIDFSRYDLILADVRWHSGTEKAFSLARLAGVTTLLD
                     ADMTPQDISPLVALADHAVFSTPGLKRMTGLQSPEEGLFQATTQTAGKVYVTLGSEGS
                     LWIEDGHLCQQEAFSVNVVDTTGAGDVFHGALAVALAEKMPTKEAIRFASAVAAMKCT
                     QPGGRAGIPNREQTESFLSLYA"
     gene            20184..20972
                     /locus_tag="ACHKIR_002206"
     CDS             20184..20972
                     /locus_tag="ACHKIR_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFO9989945.1"
                     /translation="MSIIEVTGNPRHDQLVHLIAERGYMNIEELAQLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRVSSVMNTAFEQRELSLTAEKRAIAEAVADYLPERCTVFI
                     TIGTTVEAVARALLNRRDLRIITNSLRVAQILYKNQDIEVMVPGGTLRAHNGGIIGPG
                     AVDFIEGFRADYLITSIGAIEHDGTLLEFDVNEALVARTMIKHARNTLLVADHTKFTA
                     SAAVSIGNARNVRAFFTDAPPPNSFCQLLSEENVELVVAEQEVS"
     gene            complement(21032..22297)
                     /gene="yihN"
                     /locus_tag="ACHKIR_002207"
     CDS             complement(21032..22297)
                     /gene="yihN"
                     /locus_tag="ACHKIR_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9989946.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGIIIFFVLNDKEEAPSVEVKKEDGASQNTSMTSVLRD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEAKIAENKTGAAMALGSFIGYAPAMFCFSLYGYILDLNPGIIGYKIVFGIMA
                     CFAFCGAVVSVMLVKRISQRKKEMLAAEA"
     gene            complement(22399..23379)
                     /gene="yihM"
                     /locus_tag="ACHKIR_002208"
     CDS             complement(22399..23379)
                     /gene="yihM"
                     /locus_tag="ACHKIR_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HFO9989947.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGASSVRFYPRYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEEGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYAPAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNN"
     gene            complement(23387..24097)
                     /gene="yihL"
                     /locus_tag="ACHKIR_002209"
     CDS             complement(23387..24097)
                     /gene="yihL"
                     /locus_tag="ACHKIR_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO9989948.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KSRTRDIPETLVPLITVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(24314..26137)
                     /gene="typA"
                     /locus_tag="ACHKIR_002210"
     CDS             complement(24314..26137)
                     /gene="typA"
                     /locus_tag="ACHKIR_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HFO9989949.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRSPKDD"
     gene            26509..27918
                     /gene="glnA"
                     /locus_tag="ACHKIR_002211"
     CDS             26509..27918
                     /gene="glnA"
                     /locus_tag="ACHKIR_002211"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HFO9989950.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLA
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            28092..29141
                     /gene="glnL"
                     /locus_tag="ACHKIR_002212"
     CDS             28092..29141
                     /gene="glnL"
                     /locus_tag="ACHKIR_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HFO9989951.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            29153..30562
                     /gene="glnG"
                     /locus_tag="ACHKIR_002213"
     CDS             29153..30562
                     /gene="glnG"
                     /locus_tag="ACHKIR_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HFO9989952.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNVQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEVQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            30674..30784
                     /gene="yshB"
                     /locus_tag="ACHKIR_002214"
     CDS             30674..30784
                     /gene="yshB"
                     /locus_tag="ACHKIR_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HFO9989953.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(30974..32347)
                     /gene="hemN"
                     /locus_tag="ACHKIR_002215"
     CDS             complement(30974..32347)
                     /gene="hemN"
                     /locus_tag="ACHKIR_002215"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HFO9989954.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEQQW
                     DLLFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(32536..33045)
                     /gene="yihI"
                     /locus_tag="ACHKIR_002216"
     CDS             complement(32536..33045)
                     /gene="yihI"
                     /locus_tag="ACHKIR_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HFO9989955.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            complement(33164..33330)
                     /locus_tag="ACHKIR_002217"
                     /pseudo
     CDS             complement(33164..33330)
                     /locus_tag="ACHKIR_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            33627..34259
                     /gene="yihA"
                     /locus_tag="ACHKIR_002218"
     CDS             33627..34259
                     /gene="yihA"
                     /locus_tag="ACHKIR_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFO9989956.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(34583..37369)
                     /gene="polA"
                     /locus_tag="ACHKIR_002219"
     CDS             complement(34583..37369)
                     /gene="polA"
                     /locus_tag="ACHKIR_002219"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFO9989957.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQPPVAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVVDEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            37733..38665
                     /gene="yihG"
                     /locus_tag="ACHKIR_002220"
     CDS             37733..38665
                     /gene="yihG"
                     /locus_tag="ACHKIR_002220"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFO9989958.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCESLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPISDELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQDK"
     gene            complement(38706..40136)
                     /gene="yihF"
                     /locus_tag="ACHKIR_002221"
     CDS             complement(38706..40136)
                     /gene="yihF"
                     /locus_tag="ACHKIR_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 family protein"
                     /protein_id="HFO9989959.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGIEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVAFDVDVEHGPLPI
                     TMLMRGNVIPALAAAKVKLVDNELTQPLFIAAKNKSPMEATLRFAFGGSFSTTLDVAP
                     AKYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDLVLELSPLNKVTAKSFTIDSLTRLE
                     EKKFPVGESESKFNQINIINQGEDVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFVIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLIFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKRADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
BASE COUNT        10062 a        10368 c         9803 g         9968 t
ORIGIN      
        1 ttgctcaaac gctgcggatg ggtataaacc gttgcccgac ccggtttaca aaagcccacc
       61 agcgtcaaat tacagcgttc agccacttct acagcaagtg tggtcgcggc agacaccgca
      121 aacaaaatct ctacgccgca catcgccgac ttttgcacca tttcataact ggcacggctg
      181 gaaaccagta ccgcaccttg ctgccagctt tccccttctt gtgaccggcg gcctaacagt
      241 ttgtccagcg ccacatggcg acccacgtct tcatgcccgc cgaccagttc gccagatggc
      301 aacatccagg cggcggcgtg agtacaaccg gtcagttgcc ccactggctg aaaatcattg
      361 agatgacgta atgcatcatc cagtttgttg agatcaaacg tctgggtgaa cggtagcggc
      421 tgcaccggtt ttccgatgtc attaagttgc tccacgccgc acacgccaca tcccgtacgt
      481 cccgccagtg cccggcgacg ctccttcaac cccataaagc ggcggctgga aagctcaatt
      541 tgaacttcaa gaccattaca ggaaggaacg acatccatgc cgaagatatc gcgcggactt
      601 tcgattatcc cctcggaaag cgaaaaaccg agcgcaaagt actcaaggtc tttgggcgac
      661 gccatcatca ccacatgcga aataccgttg tagaccagcg caacgggaac ttcttccgcc
      721 acctcatcta accgtgggtg ttgtaaatca tcatgccgcc ataactcaat ttgacgaaca
      781 cctgtgatat ttgtcacatt ttcgatttct ttttgctgtg tttttttcac tttctcttaa
      841 ccagataaga acacacacac caacattatg gtattctgtt acaaaccctt cctggatgga
      901 gggaaattga gccaattctg gacctttgcg gccccttccg caaagaaaaa taactcccac
      961 tccctgcaca cgcagcaagc gaatgtaaat gggacgtgac aatgtcgaaa caaggagcaa
     1021 tccatgcagg tcagcagaag gcagttcttt aagatctgcg ctggcggtat ggcaggcacc
     1081 acggcagcgg cactgggttt tgcacccagc gtagcactcg cggaaacccg gcagtataaa
     1141 ctgctgcgca cccgcgaaac ccgtaatacc tgcacctatt gttccgtagg ctgtgggctg
     1201 ttgatgtaca gcctcggtga cggagcaaaa aacgccaaag catctatctt ccatatcgaa
     1261 ggtgacccgg atcacccggt caaccgcggt gcactttgtc cgaaaggcgc tggcctggtg
     1321 gatttcatcc actccgaaag ccgtctgaag tttccggaat accgtgcgcc aggttctgat
     1381 aaatggcagc aaatcagttg ggaagaggcg tttgatcgca tcgccaaact gatgaaagaa
     1441 gaccgcgatg ctaactacat tgcgcaaaac gccgaaggcg tgaccgttaa ccgctggctc
     1501 tccaccggga tgctgtgtgc ttccgcgtcg agtaacgaaa ccggctattt aacgcaaaaa
     1561 ttctcccgcg cgctgggtat gctcgcggtc gacaaccagg cgcgtgtctg acacggacca
     1621 acggtagcaa gtcttgctcc aacatttggt cgcggtgcga tgaccaacca ctgggtcgac
     1681 atcaagaacg ccaacctcgt cgtggtgatg ggcggtaacg ccgctgaagc tcacccggtc
     1741 gggttccgct gggcgatgga agccaaaatt cacaatggcg cgaagctgat tgtgatcgat
     1801 cctcgcttta cgcgtacggc ggcggtagct gactactatg cccctattcg ttccggtact
     1861 gacattgctt tcctgtcagg cgtattgctg tacctgctga acaatgaaaa attcaaccgc
     1921 gaatacaccg aagcctatac caacgccagc ctgatcgtgc gtgaggatta cggctttgaa
     1981 gatggcctgt tcaccggcta tgacgcggaa aaacgtaagt acgataaatc ctcctggact
     2041 tacgaactgg acgaaaacgg cttcgccaaa cgcgatacta ccctgcaaca tccacgttgc
     2101 gtgtggaact tgctgaaaca gcacgtttcc cgctacacgc cagatgtggt tgaaaacatc
     2161 tgtggtacgc caaaagacgc attcctgaaa gtctgcgaat acatcgctga gaccagtgct
     2221 cacgataaaa ctgcctcgtt cctttacgcg ctcggctgga cgcaacactc cattggtgcg
     2281 caaaacattc gtacgatggc gatgatccag ttgctgctcg gcaatatggg gatggcaggc
     2341 ggcggcgtta acgccctgcg cggtcactcc aatattcagg ggctgacgga cctggggctg
     2401 ctgtcgcaga gcctgccggg ttacatgacg ctgccgagcg agaagcagac cgatctgcaa
     2461 acctacctta ccgccaacac gccaaaaccg ctgctggaag gccaggtaaa ctactggggc
     2521 aactacccga aattcttcgt ctctatgatg aaggccttct ttggtgataa agcgacggcg
     2581 gaaaatagct ggggctttga ttggttgccg aagtgggata aagtctacga cgtcctgcaa
     2641 tacttcgaga tgatgaaaga aggcaaggtc aatggctata tctgccaggg ctttaaccct
     2701 gttgcctcat tcccgaacaa aaacaaagtg atcggttgtc tgtcgaaact gaagttcctc
     2761 gtcaccatcg acccgctgaa cactgaaacc tctaacttct ggcagaacca cggtgagctg
     2821 aacgaagttg actcgtcgaa gatccagacc gaagtgttcc gtctgccatc gacttgcttc
     2881 gcagaagaga acggttcaat cgttaactcc ggtcgctggt tacagtggca ctggaaaggt
     2941 gcggacgccc cagggattgc gctgactgat ggcgagatcc tctccggtat cttcctgcgc
     3001 ttgcgcaaga tgtatgcaga acagggtggc gcaaacccgg accaggtgct gaacatgacc
     3061 tggaactacg ccattccgca tgagccatct tcagaagaag tggcgatgga gagcaacggt
     3121 aaggcgctgg ccgatattac cgatccggca accggtgcgg ttatcgtcaa gaaaggccaa
     3181 caacttagct cgttcgctca actgcgcgat gacggtacaa cctcctgtgg ctgctggatt
     3241 ttcgccggta gctggacgcc ggaaggcaac cagatggcgc gtcgtgataa cgccgatccg
     3301 tctggcctcg gtaacacgct gggctgggca tgggcatggc cgcttaaccg ccgcattctg
     3361 tataaccgcg cctccgcaga tccgcagggt aacccatggg atccgaagcg tcagttgctg
     3421 aaatgggacg gcactaagtg gaccggctgg gatattccgg actacagcgc agcgcctccg
     3481 ggcagcggcg tagggccgtt tatcatgcag caggaaggca tgggacgtct gtttgccctc
     3541 gataagatgg cggaaggtcc gttcccggaa cactacgagc cgtttgaaac gccgctggga
     3601 actaacccgc tgcatccaaa cgttatctcg aacccggctg cgcgtatctt taaagacgac
     3661 gccgaagcat tgggtaaagc cgataagttc ccgtatgtcg gaaccaccta tcgtctgacc
     3721 gagcacttcc actactggac caaacacgcg ctgttgaatg cgattttgca accagagcag
     3781 tttgtggaaa tcggggagtc gctggcgaat aaacttggca ttgcccaggg cgatactgtg
     3841 aaagtctcct ccaaccgtgg ctatatcaaa gccaaagcgg tagtgaccaa acgtattcgc
     3901 acgctgaaag caaacggcaa agatatcgat accatcggta ttccgattca ctggggctat
     3961 gaaggtgttg cgaaaaaagg ctttattgcc aatacgttga cgccattcgt cggtgatgcg
     4021 aacacgcaga cgccggagtt taagtccttc cttgtgaatg tggaaaaggt gtaacggaga
     4081 cgacatatgg cttatcaatc gcaagatatc attcgtcgtt ccgcgactaa cggtctgacc
     4141 cccgcgcctc aggcgcggga cttccaggaa gaagtggcga aactcatcga cgttaccacc
     4201 tgtatcggct gtaaagcctg tcaggtggcg tgttcagagt ggaacgacat tcgcgatacc
     4261 gtcggcaata acattggggt gtacgacaac cccaatgatt taagtgccaa atcgtggacg
     4321 gtgatgcgat tctcggaagt ggagcagaac gacaaactgg aatggctgat ccgcaaagac
     4381 ggctgtatgc actgttccga tccaggctgc ctgaaagcgt gcccggcgga aggggcaatt
     4441 attcagtatg ccaacggtat cgtcgacttc cagtccgagc agtgcatcgg ctgcggttat
     4501 tgcattgcgg gctgtccgtt cgacattccg cgcctcaacc cggaagacaa ccgcgtctac
     4561 aaatgtacgt tgtgcgttga ccgcgtggtg gttgggcaag aaccggcctg cgtgaagacc
     4621 tgcccaacgg gcgcgattca cttcggtacg aaagagtcga tgaaaacgct ggcgagcgag
     4681 cgcgtggcgg agctgaaaac ccgtggttac gacaatgcgg gtttgtacga cccggcaggc
     4741 gtcggtggta cacatgttat gtacgtgctg caccatgctg acaagccaaa tctgtatcat
     4801 ggcttgccgg agaacccgga aatcagcgaa accgtgaaat tctggaaagg catctggaaa
     4861 ccgctcgcgg ctgttggctt tgcggctacc tttgcggcca gtatcttcca ctacgtgggt
     4921 gtcggtccga accgtgcgga tgaggaagag aataatctgc acgaagagaa agacgaggag
     4981 cgcaaatgaa acgacgtgac accatcgtgc gctacaccgc gccggaacgt atcaaccact
     5041 ggatcaccgc cttctgcttc atcctggcgg cggtgagcgg gctgggcttt ttgttcccgt
     5101 ccttcaactg gttgatgcaa atcatgggca caccgcagct ggcgcgcatt ctgcacccgt
     5161 ttgtcggcgt ggttatgttt gcctcgttca tcatcatgtt tttccgttac tggcatcaca
     5221 acctaatcaa tcgggatgat atcttttggg cgaagaatat tcgtaagatc gtcgtcaacg
     5281 aggaagtagg tgacaccggg cgttataact tcggtcagaa atgcgttttc tgggcggcga
     5341 ttattttcct ggttctgctg ctggtgagcg gcgtgattat ctggcgtcct tattttgcac
     5401 ctgctttctc aatcccggtg atccgattcg cgttaatgct gcattcattt gccgcagtgg
     5461 cgttaattgt ggttatcatg gtgcatatct acgccgccct ttgggtgaaa ggcacgatta
     5521 ccgccatggt ggaaggatgg gttaccagcg cctgggcgaa gaaacatcac ccgcgctggt
     5581 accgtgaggt ccgcaagaca acggaaaaga aagctgaatg agtattcgca taatcccgca
     5641 agatgagctg ggttcgagcg agaaacgtac ggcggatatg attccgccgt tattgttccc
     5701 tcggctcaag aatttataca accgccgcgc cgagcgtctg cgcgagctgg cagaaaataa
     5761 tccgctgggt gattacctgc gctttgctgc gcttatcgcc cacgcccagg aagtggtgct
     5821 gtacgaccat ccgctggaga tggatctgac cacgcgcatt aaagaagcca gcgcacaagg
     5881 caagcccccg ctggatattc acgttctgcc gcgtgataag cactggcaaa agctgctgat
     5941 ggcgctgatt gctgagctga aacctgaaat gagcggcccg gcgctggcag tgattgagaa
     6001 tctggagaag gcatcgactc aggagctgga agatatggcc agcgcactgt ttgcctctga
     6061 tttctcgtcc gtcagcagcg ataaagcgcc gtttatctgg gctgcgctgt cgctctactg
     6121 ggcgcagatg gctaatctga tccccggcaa agcccgcgct gaatacggcg aacaacgtca
     6181 atattgcccg gtatgtggct ctatgccggt gtccagcatg gtgcaaattg gcaccactca
     6241 gggtctgcgt tacctgcatt gcaacctgtg tgaaaccgaa tggcacgtgg tgcgcgtaaa
     6301 atgcagcaac tgcgagcaga gcggcaaact gcattactgg tcgctggatg acgaacaggc
     6361 cgcgattaaa gccgaaagct gcgatgactg tggcacttac ctgaagattc tttatcagga
     6421 aaaagaaccg aaagttgaag ccgtggcaga tgacctcgcc tctctggtac tggacgcgcg
     6481 aatggagcaa gaaggctacg cccgcagttc catcaacccg ttcctgttcc cgggtgaagg
     6541 ggagtaatct ctgacgtttt ccgggtggcg ctggtcgccc ggattacagc tcatagggtg
     6601 tgacgacacc tgctattccc gcaaaatctc tcgtattttg cgaggcgttt tccagatatg
     6661 atgtaacagt tacagttatt ataagttcaa gtaaaacagt cttgtattaa taccagaata
     6721 agggcatctg gtttttatat tcaagaataa taaatttttt gtcattcctt atgctcctta
     6781 cactgatcat caacaactac tgtctcaccc ctttgcctgc caccctttgc tttacgcacc
     6841 ggaaattgct gccgctccag atattgctca accgcttcgc gtaaaattgc agatcgtgaa
     6901 accttacgcc agcccctaag cttgttcagc cgtttaattg cctcttctga taaatgaaga
     6961 aaaaccgtat tcatcgccat taccaatacc ttacatatat tactcattaa tgtatgtgcg
     7021 aaaattacgc cgaaaaatca tttaaaaaaa gcggattttt gcatgctgtt ttgtttttgc
     7081 gagacgtttt ccagaaataa taagaacgtt gcagttgttt catttgttaa tcaaaaatgg
     7141 ttgggttatc gcccaggatg agatatgggt gctataaata tattatatac agggatgttt
     7201 attttttgat tggatcttta tttgcataat ctgaatggtg tagccgaaaa atgtcagcat
     7261 cctaacagca cccggattta aggtgaaatt aatcatccat cacagaccgg tcagtactgc
     7321 taccaaccgg tcttaacttg ctcatcgtta taattttgtt ggtaacagtt cgggaaacaa
     7381 ggaattcctt ccggaacacc cccggtatca tccgatagca ttgacgtcaa tcatcggcgc
     7441 gacgatgagt ttttttaatc gcatcatagg gtggcaacgg tcagcacgat tacgggtcat
     7501 agcaaaactc ctgagcaggt ggcactacgc cgctttcaca ggagttctga atgctggtgc
     7561 tttctgtatg gcataaccat gcctctaacg gttaagtgct tgcagcacat gttgcggatc
     7621 attggcaatc gctcgcagca aagcctttgc aggcccggtc ggtgaacgtc gcccctgctc
     7681 ccagttacgc aacgtgtcta cgcttacaga aataagatcg gcaaatttag attgtgacaa
     7741 ccctgaagcg cggcgtattt ctttgatttt catagcatca acttcaaaag tccgcgaagg
     7801 ctgtcgcgtt ccttcaataa tttcattcat ctgggccatg ctgtgagtca accgctcaaa
     7861 aagtgcctta tccatttacc acctcgtatt taacaaccgg agcattgctt tttcctgggg
     7921 agacagatca tctttaatac ccttacgata aataagcagt aatctgatct cataatgttt
     7981 aacctgatga aagtaaataa ccctgacacc agcacgtttc ccctttcctc ctgacaccca
     8041 tcgaaccttt cttaacccac cagtttcagg tattacctca ccatagtcag gattcaaggc
     8101 caggaaaaac tgaaaacgac tgaattcatc gtcggtcagc agtttttgaa catcttcagt
     8161 aaaaatttcc gtttcgatga agagcataat tatacgccat tggcttagag taacaatcct
     8221 ttttgtacgt cattgacgta cccttgaaac cactagctta ctgatagtgc tccagcaaaa
     8281 ccacgtctct gccgggtttg tctttacatt tgcgaggcgc tttccagatt tttcacgccc
     8341 gtgaatttcc ctgtatccat ccagtaaaaa cgggtgtata aaactgtaca ttcatacagc
     8401 aaaatgagtt ctactatggc acaggaaaaa ggaattgcag agttagtcga agagtttatc
     8461 gctgcgggtc gcccctcatc gcgtcaacaa agtattgcgc agcgcaggga gggctatatc
     8521 gccagcgctg tccttgcggg tgagacagaa acgcgggtag atattcagac tatcgaactt
     8581 gaagcgatga ccttacgaat agtgtcaccg ctgaatgccc cgacattact cccgacgata
     8641 atttattact acggtggctg ttttgttagc ggcgggttcg caactcacga caaccagctg
     8701 cgccagttag cctattacgg gcaatgtcgg gttattgccg tccagtatcg gctggcaccg
     8761 gaacatactt tccccgccgc ccatgatgat gcgcaacgag gtgcagaatt agtctggcaa
     8821 cacgccgaac gactcggcgt tgataaacaa cggataactc ttgccggtga cagtgcgggc
     8881 ggacatctgg cgctggtaac tgctttgcga ttaaagcgtg ctggcgagtg gcaaccggca
     8941 caactcatct taatttatcc catgcttgat gcgactgcgc atttcgaaag ctacatacgc
     9001 aatggtcacg attacatcat cacacgcgac actttactga gcggctttga gatgtacctg
     9061 ccaggcattg aacgcaggca tcctgaagcc agccctcttt ggcgcgatga tttcaatggc
     9121 ttgccacctg tacatatcat taccgctgaa tacgacccac tttgcgatga aggtgaagcg
     9181 ttgtatcacc gcatgacagg gcaaggagtg caatgcacct gccaacgcta ccttggcgtt
     9241 atccacggat tttttcagtt aggtggtatc agtgaggctg cgcgatcggc gctacgggat
     9301 gttgcctgga gagcgggaag gtaactccgg accagggccc agaaaaggtt ttcagattga
     9361 aaaccttttc agtcaagtaa aacgccccta ctcttcttcg ttcccgccct cttcatacgg
     9421 gccaaatggc ttcgcgggca gaacgatata cgtgccttca aacactgcac ccggcgtctc
     9481 gtcgccaaag atttctactt gcatctgcac ccgtgctttt cgtccgcgcg ccagacggtc
     9541 gagatcgccg cttaaggcac caaggtcggc taccgcatga ggtttgccgc taatcggctt
     9601 gctgtagcgg atatgcgcat ccgccagaat aatcgttccg ccgaggtggc gttcgcgcag
     9661 catcagccag ataagccccc aaccggtgag cgttgccagc gagaataaac tcccggcaaa
     9721 cagcgtgtga tgcggattct gattgcctgt ttccggcatg gtggtgataa atttttgccc
     9781 ggtatattgc tgaatgcgca cgcccatttt ttcactcagc gggatgtgtt cataccacgc
     9841 ctgttgcagc tgcgcgcacc agtcgccgcg atgcaaaata tcatccagag tggcaacggg
     9901 tttaatcatc aaaaaatggc gaatcggcgt ggtggttggc gtggtgattt ctccctgatt
     9961 aacaaacccc agcttggcga aaaactccac cgcgtcttcg cgggcgctac aggtcacgcg
    10021 cttaacacct tcctgacgcg ccacagattc gagggtcatc gccatcagcg ttcctaaccc
    10081 tttgtcctgc acgtcgggat gaacggccat aaagcgaatg gacgcttcat tgtcggcatt
    10141 aatatacagt cggcctaccg ccaccagatt accctgctcg tcgacgacca tctggtgatg
    10201 cgccatcgca tcccacgcgt cgcgttccga accttttggt tgatgcaggg gcttacgcaa
    10261 catttcccag cgaaactgat agtaacgctc taattcttct tctgtttgtg gaacccgaag
    10321 gtgatacata gcgatactct ctcttgtttc ccgtgacaac cctggaagct ggctcatacc
    10381 tgcaaccaga atgtcacggg gccatcattg accagcgata cctgcatatc cgcagcgaag
    10441 cgtcctgttt gcgtgttcat ctcttgctga cggcagcgtt cgacgaaata gtcatataac
    10501 gcctctgcgc aatccggtga tgcgcctttg gagaaacttg ggcgcatccc ccgttcggta
    10561 tctgcggcaa gggtaaactg ggaaaccacc agtacactgc cgcccgcctg ttgcacgttg
    10621 agattcatct tgccttcggc atcgctaaag atgcggtagc cgagcacacg ctcgcacaga
    10681 cggtttgctt tctgttcgtc gtcatccttt tcgacaccca ataacaccaa aagtcccgcg
    10741 ccaatttcac ccgtcacttc tccctccacg gtgacgctgg cacgggttac gcgttgaatt
    10801 aatgcaatca tggttcgtcg tcttcttctt gttcagcagc ttgttttaat ttgcggtatt
    10861 ccccgagagt gacagtaatt tccgcgccaa gcaagacgat acaccatgtc cagtagaccc
    10921 aaacaaagag aatggggatc accgccagca caccgtaaat gagctgatat gatgggaaca
    10981 tggtgatata aagcgcgaaa cctttctttc ctgcttcgaa caggagcgcg gcgacaaacg
    11041 cgccgacaat cgcgtcgcgg ttaggtacac ggatggtagg aacgatgctg tacagcagcc
    11101 agaaagagat ccacgacaac agcagcggaa aaatacgcaa cacattatcg atgacagtat
    11161 tgagatcgct cgcccagcgc agagagagca gataggagct gatcgccaga ctggcccctg
    11221 ccaacagcgg ccccagcgtt aaaatcatcc agtacacggc aaacgagtaa atttttggtc
    11281 gcgctcgttt actgcgccag atggtattca acgcgctatc gatggagtac atcaacaata
    11341 acgccgtgac gatcagcccg cacgccccga cggcggtcat tttgttggaa ttggcgacaa
    11401 actgttcgat atatcgctgg ataacatcgc cagtagcagg cagaaagttg gcaaaaataa
    11461 agtgacgcaa ctgaatgctg acgtcggaaa acatgggaaa agcggcgaaa agcgcaaaaa
    11521 caacggcaac cagcggcact aatgagagca acgacacata ggcaaggtta cctgccaggg
    11581 ttgtcatgtt gtcctcatca atgcgttgcc agagtagttt tagccaggcc catagtggac
    11641 gggtacgatg cctggctttg tcctgaatgg tttttagcat aacaccttcg cgaaatagtc
    11701 cgggatggtg gttttatctt tcaccagaat actagtaatg cctagctgat tagctccttc
    11761 tatattatcg gcgttatcgt cgaaaaagac cgtatcgtct ggtgaaaaac cttctgcctg
    11821 caaaacatgc tgataaattc gtgcttcagg tttgcgcata cccaagtctt gcgacagata
    11881 gatatggtca gcagcatcac ggatttccgg gtattcctcc ggccagaagg tagtatgcag
    11941 gcggttagta ttggaaagca ccaccacgcg atgcccctgc tcacgcagtt tatgcatgat
    12001 ggcgatcact tccgggcgca gcgcaacaaa taccgcctgc caaccgtggg agaactgctc
    12061 atagcttagc ggcagggcca tctcatggca cagtgcctct gcgaacgctt cgtcgctaat
    12121 ttccccacgc tcatgctgat gaaacgcctc gcccatgtgg aaactcttct taagcgtagc
    12181 cagtggaaca cgcgttaaat cgctccaggc ccccagcaca cggttaaagt cgatatcgac
    12241 aatcacatta cctaaatcaa agatatagag catttttgcc tcctttcgcg ccacaagaaa
    12301 ttaactgtag cgggaaagga tgggtttggc tatgtggcaa taagggtaat cttgtgatgc
    12361 tgattgcgaa aaagcagagt aatttcactg gctttttcgc aatagatgaa tggttaagat
    12421 tcaggtgtaa attgccaacg agtttcgatg accagcggct tatctggtgt cacaaattcc
    12481 ggcgttagat ttattgcgtt aggtgcctga gtcagcgggt taatacaggt ggcatcgggc
    12541 tgtttatcaa ataccaccag actgtttgca gaagaggtca tggtcatcgc gagtttacca
    12601 ggccacagta acttaacctt caccccgtca taaaaaccaa aacagtcatc ccagggaccg
    12661 acttgaggag agatacgatt tccagttggc agttcatcac tacccgcttc ttcttgccag
    12721 tccgcatcaa aaagcacctg caacgattct gtattttgcg gagtcagctt tttcgcaaac
    12781 cacggatgcc agcctgcgga ggccgggaat gtatcggcat atgaatggac ttccagttgc
    12841 aaaaccagcg catcgttctc cagcaaaaaa gtctgaatca cttccccttg ccagggccac
    12901 ggtgaagcca ggggcatacg taaagtcagg gaatctgctg ttttgtcgat gatttcccac
    12961 gttgagtaac aagccatacc atgcaacgca tgtggtggtt tattggcggg taaagaataa
    13021 cattgcccac cagcattcag cgtcgcattc cccagtcgcc ctgcccacgg caccattggg
    13081 aaacagccat actgaaaagc tctgcgctgc ggctgccatt ggcgtaatac ttcagaacca
    13141 aatgccttta acgaggttat cctggcacca tcatgcggat aaatctgcag gtgaatattg
    13201 cctttttcca gcgtcactaa ctctcctaca ggcccaggcc atggcgcgtc aaacgcctgt
    13261 tgtagcgcca ggtaatgctc gttttttcgt tcagttttca tgagattgtt ccctgatgta
    13321 gcggtaagaa gcggtttctt gttcatcggt tggctcaatc agccagacgc aaacaaagga
    13381 aagaactgag acagcaaata aaaacagagc gacataccac ggttttcctc cccctagcgc
    13441 cagcaaactt gaggcgataa gggggcttag tccgccaaga atcgcggaaa attgataagc
    13501 aaaagaaagg ccggtatatc gtactttggt accgaacatg cgggtgaaaa gtgttggctg
    13561 cacggcaaac atcatcgtcg gacctaagtt gtagccaagg atcatactgc accagatgat
    13621 gagcgtactt ttgctatcca gtaataagaa gaaaggaaac gcgaacagga cacaaaatcc
    13681 tgctcccgac aaatacagac ttttttgacc tatacgatct gataaccatc caaccaatgg
    13741 acaactgaaa ataccgatcg ccgaggcaat caacataccc gtcaggggaa tatctcgcga
    13801 caatgctaat tggctggaaa tatagacaat accaaaggcg ttaataatat tagaggagac
    13861 actttccgcc agcctcgcac ccagtgccaa taaaatattt cgtggatgtt ttttgaataa
    13921 ttcaatcaac ggaggaacgg acttttcttt attattcgtc gttttttgct tttgaaaatc
    13981 cagcgtttct tcggtatgca ggcggataaa cagcccaacg atcaacatca ccgcagaaag
    14041 ccaaaacgga atgcgccatc cccaggagag aaactgctcc gaagtaagaa aatggttaca
    14101 gagagcaaaa ataccggttg ccagcataat acccacagac gcagccgttt gcgggagtga
    14161 acctaaaaat ccgcgtcggg attcgggcgc tgattctatg gtcatcaatg ccgctccgcc
    14221 gtactcgcca ccaagaccaa agccctgaat caaacgcagc accattaata caagcggtgc
    14281 ccaaatgcca atttcctgat acgtcggaat aaaaccaatc aaaaatgtgg agcaaccgac
    14341 aatagccagc gtccagataa gtgttatttt tcgcccgatt ttatcgccaa aatgaccaaa
    14401 gacgatgcca cctaaaggac gagtcagaaa accaacacca aaggtcgcaa aggaagctat
    14461 aagacctata aaaggatcgc tgtctggata aaacagcggt gcaaatacca gtcctgctgc
    14521 cgtgccaaaa ataaagaagt cataccattc cattaacgcg cctgcgaaac tgcccataac
    14581 gacacgtctc atggctttac cggaaagccc ttcacgcggt gcgggtaact tttcagacat
    14641 aattttcctc tcctggtagg cgagaaatct gggatacgaa ccactaaacg tgggcagttt
    14701 gaattaatgc gcatcggctt aaaccgatgc gcattaaatt atttcttcaa ttcagccaga
    14761 acctctgcgt cctcagcatc aacatttgca cccagtggtt cccaattaat caatacacag
    14821 gtcaggaatc ccaggaatgg cacgattgcc aaccagtaaa acgcttcagt tttcaacgca
    14881 ccccacagca tcggccagaa aatgagtcct gcaattgccc ctgtgcgcat aatagcgcgg
    14941 gcaaatccga ccccagtagg gcgaatagcg ggaggataag agagtgtggc aatggtcatg
    15001 cccagcccac caggacccgc tgaatggaag aaaataattg atcccaataa ccctaaagac
    15061 agccagggat tagtcgttgc cagtgcgcct aacgacagca atgctaaggt cacaacagca
    15121 aatccataca tcgactgccg gcgagtaccc agtcgttgga gaataaacga gccgatcatc
    15181 ccgcctgtca ccccacaaag ggcattcacg acggcagaac cggttaacgc gccagtaagc
    15241 ccgccactta ttattcccgc caacgtaaga ggaagataaa cacctacggc gttatattgc
    15301 cacgcctgca tggttgccac gacacagcca agaatggtgc gtttgcggta acgatcatta
    15361 aataagatgc catatccacc actgtattta ttctctgctt tttccgagaa tttcgcttct
    15421 gttgtacccg atgccgcaac gcgagcctga acaccataac gttttcccag aatgtcgcac
    15481 gcttcctggt aacgcccgac gcgtgcggcc cacattgcgg attcaccaat aaagaagtaa
    15541 cgcaaaatca tgaagatgac tgcaaaaata gctccgatga atattccgta acgccacaat
    15601 tgttcttcag cgatacccga cagaagcagc ggcaaaagca ccaaataaac aacggttgtc
    15661 gaaacatacc aggccatttg ccagaggttg accgacgtgc cggttttctt cgacgttgaa
    15721 ccacgaagct ctgccacggc attggttgcc aaagggaagt cgatgcctaa accaaagccc
    15781 atgatgacgc ggcacaccag cacccaccag atactgggag caatagctac cgctgcagca
    15841 ccgatggtgc acagccccat gccaataata aatgcccgct tttgcccaaa gcggtttatt
    15901 actcggttcg ctaataaacc gccaattaac gcggcgatta atactgatgc attgacggtt
    15961 gcagcaagac ccggcgaaat gccgaattga gcggcaatgt atttattacc aaaccccacc
    16021 atcgccgcct ggtaggcatc aatcaatatg ccgcccaaag caataaaaat aacgccgata
    16081 ctggtgttga ttgcagagtt cttattaaca atatcaatta cgtcctgtgg ccgactaatt
    16141 gaataagttg caggactggt gatttgactc atcgcagtgt cctttttaat tgttgtaggg
    16201 tggcgctgac gtgacgcagc gccagacaga gatttagaaa atcattccgg cgcaaattgc
    16261 agcatgacct ttatggagtc attttgctgc gcgcgttcga atgccgccgc tgcatcctca
    16321 atggcaaata aatcggtaac cagttcactg gtatcaaacc gcccatccgc cagccattcg
    16381 ataacagcat cgaaatcttc agggatgtac atgccagagt tgagcaagtc acgctcgaaa
    16441 cgttgcatca tcggtaatgg tatttcacgc ggcccacttg gtacgcccat gcacacgacg
    16501 gtgcctccgg catatacacg ggtacatgca tcgttaagcg tggcctgcgc tgcaacgaca
    16561 tcaatcgccc ccgtaaatcg cacatctgcg gccagctcac ctggtgccca aacttctgct
    16621 gcgcccagtt ttaacgccag cgcgcgtttg gcagcgtcag gttcaatcac ggtaatctta
    16681 cctgccccca taatgcgcag tgcctgcacg atggataagc ctatcgttcc ggcaccaatc
    16741 accagtacgc tttccagaga agctttcggc attcggttaa cgcagtgacg agcacaagcc
    16801 gccggttcgg caaaggccaa cacttttagc ggtaaagagg ctggtgcgac atggcaatta
    16861 cgcgcaggaa caatgtgttg tgagcgagca aagcccggtg cgcggaaacc agcaacctgc
    16921 ggtggttcac aaagattaaa acgtcctgct ttgcaggctc ggcattccat gcaagccatg
    16981 atgggatcga ccacaacatg atcgcccggc tgtacatttt taacgtcgct gcccacttcc
    17041 gtaacgcgcg ctgcaatttc atgaccgggg acgacaggcg gtttggcaaa cggatgaccg
    17101 cctttcagaa catgcagatc cgaaccgcat atcccgtaga acacgggcgc aatccgtact
    17161 tcgtgcggtt taagttgctg gtgtggtact tcaacatatt gagtggtgac tttcccaata
    17221 tctgaaaata aaacctgggt aattttatcc attgttattc tccagaatcg atatccatag
    17281 gggattagcg ttgcgcgata ccttcatcga caatttcagc cagccgttgc aagcgtggaa
    17341 cggagacatc gcgcagcatg gtttgtggat cttgtgcacc aacaaccgac gcccaaaccg
    17401 ctcgtccagc caggaagccc gacgcaccac ctttcatggc aatacttact gcacggccaa
    17461 aaatatcggc atcaacgcca gaagaaagaa ttacccacgg catcttcatc tgatcgttaa
    17521 gttgctgaca ggcagcaagt aaggtttttt catcgccttt gccccctaat ggcatctcgg
    17581 ctttgtaaag atcagcctct gttccaccaa gctctgcggc ggcggcaaca atggcgcttt
    17641 cacgatcaaa gtcccaaccg cgacgaggtg gacgcaccac aggttcaata atgctaacca
    17701 gcccggcact acggcagcga cgaacaaatt tatcaaccat tgccagacgt tcctctgcgg
    17761 gttcatcttc acgccagagt acaagtaatt tcattgcctt agcccccatt tcacgggctt
    17821 tatgcgggtc gacggacata tctatttcaa cgctatcaac cgggatgcca ttacctggga
    17881 taaaaagatc ggcagcgaca atcaggccgc aagaattagc cacagcgccc tgctcgacta
    17941 tttgttcaag acaaaattgt ttatccgcca gaacggcaga agcataagga gaaaggattc
    18001 gggtcgcggc aactttaaaa tcggtgagta cactgtcggc gatgggtttt ggctgccctg
    18061 ctgccgcaaa catcaggcgc atggcctcac gctgatcgac agctaacatg gcgaatccac
    18121 cacctgggcg ggagatatct ttcagcgtaa atggggtgaa gtgggatgac atagaaactc
    18181 ctgttaatag ataaaattaa agaccagcct gctggcgaac ctgatttagc agggcgctcc
    18241 agtcctgccg ccctttgccg tcttgacttg ctgcggcgta ataactttct gctgcaatcc
    18301 ccatattgag cggcgccccc gtttcccgag cgacgtcaac tgcaatacgc agatctttca
    18361 atgccaggtc caccatgaag gcgggagaaa gatcgccttt cagcactttg ccaggccagg
    18421 tggtcgtaaa atgtcctttc cctgctgccg taccactcat caccttaatt gcgacatcaa
    18481 gatttaatcc cagtgactca cataacacag cggcttcgga ggacagcgca ttaagcgcaa
    18541 tgctcatata gttgttaatt agcttcacgc ggatccccat tcctgggcca ccagcctcga
    18601 ccagctcatt acccatgcac atgagaatcg ggcgtgcacg cgcaatctgc tcatcagtgc
    18661 cgcccgctaa aatcagcaat gtgccatcaa tcgcattcac tgacgttctg cccacggggg
    18721 cgtccatcat attgatgcct ttttcttgta attcccgtat taatgcatcg gtctgtaagg
    18781 gatgaatagt cgacatatca atcactaacg cgtcagatga aacagtttca cagacgcctt
    18841 tttcccccaa cagcacctgt cgaacaatgt ccccattggg aagcatagtg atgataaatt
    18901 cagcgcttgc tgccgcttcc gctggggttt gtgccgcagt ggccccctgc gtaactagcg
    18961 catcgacggc ctgtgtattg acatcaaata cctgtaatga atgccctttt tgcaatagat
    19021 tgttagccat tgggctaccc atctgcccca gcccgagaaa tgcaatggtt gccatgaata
    19081 ctctcctgtc tgacattgac aaaataatga caattgaaaa ttcgttttcg tctgtttttg
    19141 agcatatttg taatttatgt tcaaaaacac gacacgcatc actaatttga tcatttgtgt
    19201 catttaaatt gaccaaagac gacaaaatta aacaggacaa gacaaatatg accagaattg
    19261 catgcgtggg tatcacagtt caggacagga tttattcttt gcccactctg ccggagggcg
    19321 gcgggaaata tcaggcaaat cattatcttg agattggtgg cggcccggca gcaacagccg
    19381 ctgttgcaat cgcaaagctt ggcgtggagg ttgatttcat cggtcgcgtc ggcgatgaca
    19441 gttgtggaaa taccttactt gcagaacttg agggttgggg cgtcaatacc gcattttgcc
    19501 gccgatatcc taacgcccgt tcgtcgcaat cagcgatcct cgttgatcag catggcgaac
    19561 ggattatcgt taattatcca agtcctgatc tcggtactga tgccgaatgg ttagaagcga
    19621 tcgatttctc tcgttatgat ctgattctgg ccgatgttcg ctggcatagc ggtactgaaa
    19681 aagcattctc gctagcgcgc cttgcgggtg tgacaacgct tttagacgcc gatatgacac
    19741 cgcaggatat ttctccactt gtcgcgcttg ccgatcacgc cgtgttctca acacctgggc
    19801 tgaaacgcat gactggcctg cagtcacctg aagaaggttt gttccaggca acgacacaaa
    19861 cagcaggtaa agtgtatgtt acgctgggtt ctgaaggatc gttgtggatt gaagatggtc
    19921 atttgtgtca acaggaggca ttttctgtca atgtggttga taccactgga gcaggcgatg
    19981 tttttcacgg cgcactcgcg gtagcgttgg cagaaaaaat gccaacaaaa gaggctattc
    20041 gttttgccag tgcggttgcc gcgatgaaat gtacccagcc tggcggtcgg gccggcattc
    20101 ctaatcgtga gcaaaccgaa tcatttttgt cactttatgc gtaaaatggt aaacatctac
    20161 cgaggagggg caagggagta ttcatgagta tcatcgaagt gacaggtaat ccacgccatg
    20221 accaactcgt tcatctaatc gcagaacgcg gttatatgaa tattgaagaa ctggcacaac
    20281 tgctggatgt ttcaacgcaa accgtgcgcc gggatatccg aaaactcagt gagcagggct
    20341 tgatcacccg tcatcatgga ggggccgggc gcgtctccag cgtcatgaat accgcttttg
    20401 agcaacggga actttcgctc accgccgaga aacgggcgat cgctgaggca gtcgccgatt
    20461 accttcccga acgctgtacc gtctttatca ccatcggtac aaccgtagaa gccgttgcca
    20521 gggcattact caaccggcgt gatttacgca ttatcactaa tagcctgcgt gtggcacaga
    20581 ttctttataa gaatcaggat attgaagtga tggtgccggg aggcacttta cgcgctcata
    20641 acggtgggat tatcggccca ggagccgtgg attttattga aggcttccgc gcagattatt
    20701 taatcaccag tataggtgcc attgaacatg acggcaccct actggaattt gatgtcaatg
    20761 aagcgttagt cgcgagaacg atgattaaac atgcgcggaa tacattgtta gtggccgatc
    20821 atacgaagtt taccgcgtct gctgccgttt caattggcaa tgcacggaat gtcagggctt
    20881 tttttactga tgccccgcct cccaattctt tctgccagtt gttaagtgaa gagaatgttg
    20941 aactggtggt tgccgagcaa gaagtatcct gaaattgaaa cttcggttga tacggttttc
    21001 gttggccgga ttaagcacat ccggccacta attaagcttc agccgccagc atctctttct
    21061 tacgttggct aatacgctta accagcatta cggaaaccac cgcaccacag aatgcgaagc
    21121 aggccataat gccaaacacg attttgtagc caataatccc cggatttaaa tccagaatgt
    21181 agccatacag actgaagcag aacatcgccg gggcgtaacc aataaagcta cccaacgcca
    21241 tcgccgcgcc tgttttattt tcagcaattt ttgcttcgcc gataggtgca aaaaatacgg
    21301 ctcgctgtgt gaagactata gcgccaaagc ccagcgtaca tgccatccct aaatagaccg
    21361 gcatactttc gtgcggcagc ataatcaaca gtacgagcgc agcggtactt atgataaagg
    21421 tgtagcatag atatttactc ggcgatttca ggatcttatc tgaaatcatg ccaccaatcg
    21481 gtcctccaat cattttcaga cagtattggt taatgatgcc gtaagccccc accagagcaa
    21541 cgggcaatag atagatgttt ttcaggaatg gaatgaagaa tgttaggcca cagtaaaccg
    21601 cgtaaacgaa gaagacgttg aaagcgataa gccagatggt tttatctctc agcaccgagg
    21661 tcatactggt gttttgcgat gctccgtctt cttttttcac ctcaacggac ggtgcctctt
    21721 ctttgtcatt caggacaaag aaaatgataa tgcctacggc aatcacaata agggaataga
    21781 accagatgcc tgctttaaaa cccaataagc cactgccaaa ccaggtaaat actgccaacg
    21841 cagaaaatgc caccacggta tcgacaatgc cacgccctgt ttcgaagaag ccaaacaacc
    21901 gaccttgttg ttcgctattg cccaatcgac ttaccgactt gagcaagacc ggccagttca
    21961 tcatgtcgca agtaacgcca aatagcgccc agacaaagag gatgccccaa taccccggca
    22021 ttgtcgtcaa ataaacaccc agtaatcctg tcgcaatgag tgagaatgac atggtgtatc
    22081 tgcgcggtag tttatcggca aaataaatag acagaaaaaa gcccactgtg gtgacaaatg
    22141 agtttaccga catagcatta ccaatttgac cattggtcaa atggaaatat tcctgcatcg
    22201 ggatataaaa cgcatctttc agagacggta atttataaat tgtaccgcca cacaatgtta
    22261 atagactaaa taacgcccat ttctttttcg tgagcataac atcccctaag cgatttttaa
    22321 aatcactgtt ttaaaggcaa taagataccg accattcata tggaatggtt ttattaacag
    22381 cgtatgagtt aaaacttatt agttgttcag aagatggttt gcctcttgtt tgatttgttg
    22441 tagtacatta cgcacatgat ttatctgatt aattgcatct tctttttctt tacgtaaccg
    22501 cgcatccagt aaatgatttt ctggcaatgg tgtttcactc atctggcgat aagggatcca
    22561 cggattatca tcttcgtctt caaagcggaa cgccggcgca tagtaatcaa cctcttcttc
    22621 caggccatat gccgtcacct gcggctcatc atcacccagg cagataaggt gcgttaataa
    22681 cgctttgaac ggcatatcac cctgacctga aatacaggct ttatgaccca ggccaccctc
    22741 ttctttaacg atcaaggcat ctttgatatg gacctgggta atatgcggtg ccatcgtttt
    22801 taaagcgtcg atgggatgct catttgcatt gatcatgttc gcaaaatcaa acagtaagga
    22861 aagagattcc atctcacttt ctttgaccag cgacaccagc tcatggcttt ttaaatcttc
    22921 atgctgttcg atcgtaaaag tcaggccgct gtcctgatac gtttcccgta cataggcaat
    22981 gtcgttagcg atgatcgata atacgtcgcg caaattacct tcataacgtg gataaaaacg
    23041 tacggacgat gccccagttt tcaacgcaat ggcgacggct tcgtcaatag atgccttatc
    23101 tgaagcgctg gtttcaatat gaatatcaag gttcagacgg cgggctttat ccccaaaggc
    23161 agagagttct ttatcgtcca tattaccaag agaaaaacgc tcgccatcca gaacatggat
    23221 tttgacgcca cgtagattat tttcacttgc gatatcaagt aaatcagcag gcaacactct
    23281 ttccagccgc atatttaaat gaaaggcata agcatgtaaa taaagaggaa gagtgtcgac
    23341 acgttgtaga atctttcttg cgttatttat tgtcaccata aaacacctac agattaatga
    23401 tgaggtctac ggcctcaaat cgccaatatt caatatctat ctggacaata ttgccttgct
    23461 ggtcggcgcg atgcttttca attaagatcg ctggcatacc cgcagaaccg ccaagatact
    23521 tgctgatatc gcccggcatt ctgacgggtt tgaaaatcaa tttttttact accgtctctt
    23581 tttggtagca cttttcccag actgcagaaa atgagtggtt ttcaagttgt tcaataaaac
    23641 ccggggcaac ttcaggatta atatatgttt cgtgatagaa aactttatgt ccttccagcg
    23701 ctccccagcc ggtgatgcga tagagttcag ttgatggtgt cactgtaatc agtggcacga
    23761 gcgtctcggg aatatctctg gtacggcttt tctcggtaaa cccccaggaa ggctcccgcc
    23821 cttgctcaat tgcagcccgc tgaaagctcg ccgacagctc tggactgtaa ttaaatcgcg
    23881 gctgggtcac gaaccaccct ttacgatcct tacggaagat tttggattct gcctcgaggt
    23941 tcaacaacgc ctggcgcagc gtcatacgtt taatgcccag cagttctccc agctctcttt
    24001 ctgaagggag ttttccaccc ggcgtggtga tgccatcttt caaccaccga tccagtttct
    24061 cttttgcatt ttctacggtg gattgattct ccgccattgt ttaatccttt tggtttaaac
    24121 cagatgaggc atcttacgct tacgccaaaa aagaaagcaa ttaacggcgt atgactttgc
    24181 agataattgt gacaaagctc tctattgacg taatcacaga cttataaggg agatactcgc
    24241 attggtgtgc gctcacgaca ggtaaaaaaa aacctgccag cgatggcagg ttttttatga
    24301 ctgaaaagcg aaattaatcg tctttcggtg agcggttggc gcgacgacga tcgttttccg
    24361 tcaggtgacg tttacgaata cggatagagg tcggagtcac ttctaccagt tcgtcatcat
    24421 cgatgaactc cagagcttgt tccagagtca tgcggatagg cggaaccaga acaacggctt
    24481 cgtcagtacc ggaagcacgc atgttggtca gtttcttacc ggtcaggcag tttacagtca
    24541 ggtcgttaga gcggctatga ataccgataa tctgaccttc gtaaacttct gcaccgtgac
    24601 cgaggaacag cttaccgcga tcctgcagac cgaacagcgc gaacgcgacc gctttaccct
    24661 gaccgttaga gatcagtaca ccgttctgac gctgacccac ttcgcccgga cgtacgtcgt
    24721 cgtagtggct gaaggtggag tacagcagac cagtaccgga agtcatggtc atgaactcag
    24781 aacggaagcc aatcagacca cggcttggga tcacgtagtc gagacgtacg cggcctttac
    24841 cgtctggatt catgtttttc aggtcgcctt tacgttcgcc cagcgcctgc atcacagaac
    24901 cctgatgctg ttcttcaacg tccagcgtca cgttttcata cggctcttgt ttacgaccgt
    24961 cgatttcacg gaagattact ttcggacggg ataccgccag ttcgaaacct tcacgacgca
    25021 tgttttcgat aagaacagac aggtgcagtt cgccacgacc agaaacgcgg aacgcatcgg
    25081 cgtcttcggt ttcttctacg cgcagcgcaa cgttgtgtac cagttctttg ttcagacgat
    25141 ccaggatctg acgagacgtt acgaacttac cttctttacc gcagaacggc gaggtgttaa
    25201 cgcagaagaa catagaaacg gtcggctcat caacggagag tgccggcagc gcttcaacgt
    25261 tttgcgtgtc gcaaacggtg tcagaaatgt tcagttcgcc caggcccgtg atcgccacga
    25321 tatcgccagc ttccgccaga tcggtttcga tacgttccag accgaggtgg cccagcactt
    25381 taccgacttt cgcgttgcgg gttttgcctt cgctatcgat gatagtaacc tgctggttcg
    25441 gcttcacttt accgcgcttg atgcggccaa tgccgataac gccaacatag ctgttgtaat
    25501 cgagctgaga aatctgcatc tggaacggac cgtcaaggtc aacgtccggc gcaggaacgt
    25561 ggtcaacaat cgcctgatac agcggggtca tgtcttccgc catatcttcg tggtccagac
    25621 ccgcgatacc gttcagcgca gaagcgtaaa cgatcgggaa gtccagctgc tcgtcggtcg
    25681 cgtcgaggtt aacgaacaga tcgaatacct gatccacaac ccaatcagga cgcgcgccag
    25741 ggcggtcaac tttgttgata acaacaatcg gcttcaggcc gtaagcaaac gcttttttgg
    25801 ttacgaagcg cgtttgcggc atcgggccgt caaatgcgtc aaccaccagc agcactgagt
    25861 ctaccatgga cattacacgt tcaacttcac caccgaagtc ggcgtgcccc ggggtatcaa
    25921 cgatgttgat acggtaatca ttccatttga tagcggtgtt tttcgcgagg atggtaatcc
    25981 cacgctcttt ctccaaatcg ttggagtcca tcacgcgctc ttgggtttcg gcacgagagt
    26041 cgaacgtacc ggattgttgg agcagcttgt ctaccagggt ggttttacca tggtctacgt
    26101 gcgcgatgat ggcgatatta cgcaattttt cgatcacaac tttgcctcag gcattagaaa
    26161 tagcgcgtta ttgtacacgg attaatcgca ctacaaaaca ggatcacaaa catcctccgc
    26221 aaacaagtat tgcagagtcc ctttgtgatc gctttcacgg agcataaaaa gggttatcca
    26281 aaggtcattg caccaatatg gtgcttaatg tttccattga agcactatat tggtgcaaca
    26341 ttcacatcat ggtgcagccc ttttgcacga tgttaagcat gataacgcct tttaggggca
    26401 atttaaaagt tggcacagtt ttcgctttat ctttttacgg cgacacggcc aaaataattg
    26461 cagatttcgt taccacgacg accatgacca atccaggaga gttaaagtat gtccgctgaa
    26521 cacgtactga cgatgctgaa cgagcacgaa gtgaagtttg ttgatttgcg cttcaccgat
    26581 accaaaggta aagaacagca cgtcactatc cctgctcatc aggtgaatgc tgaattcttc
    26641 gaagaaggca aaatgtttga cggctcctcg attggcggct ggaaaggcat taacgaatcc
    26701 gacatggtgc tgatgccaga cgcatccacc gcagtgattg acccgttctt cgccgattct
    26761 accctgatta tccgttgcga catccttgaa cctggcaccc tgcaaggcta tgaccgtgac
    26821 ccgcgctcta ttgcgaagcg cgccgaagat tacctgcgtt ccactggcat tgccgacacc
    26881 gtactgttcg ggccagaacc tgaattcttc ctgttcgatg acatccgttt cggatcatct
    26941 atctccggtt ctcacgttgc tatcgacgat atcgaaggcg catggaactc ctccacccaa
    27001 tacgaaggtg gtaacaaagg tcaccgtccg gcagtgaaag gcggttactt cccggttccg
    27061 ccggtagact ctgctcagga tattcgttct gaaatgtgtc tggtgatgga acagatgggt
    27121 ctggtggttg aagcccatca ccacgaagta gcgactgctg gtcagaacga agtggctacc
    27181 cgcttcaata ccatgaccaa aaaagctgac gaaattcaga tctacaaata tgttgtgcac
    27241 aacgtagcgc accgcttcgg taaaaccgcg acctttatgc caaaaccgat gttcggtgat
    27301 aacggctccg gtatgcactg ccatatgtcg ctggctaaaa atggcgttaa cctgttcgcg
    27361 ggcgacaaat atgcaggtct gtctgaacag gcgctgtact acattggcgg cgtcatcaaa
    27421 cacgcgaaag cgattaacgc cctggcaaac ccgaccacca actcttacaa gcgtctggtc
    27481 ccgggctacg aagcaccggt aatgctggct tactctgcgc gtaaccgttc tgcgtctatc
    27541 cgtattccag tggtttcttc tccgaaagca cgtcgtatcg aagtacgttt cccggacccg
    27601 gcggctaacc cgtacctgtg ctttgctgcc ctgctgatgg ccggtcttga cggtatcaag
    27661 aacaagatcc atccgggcga agcgatggac aaaaacctgt atgacctgcc gccggaagaa
    27721 gcgaaagaga tcccacaggt tgctggttct ctggaagaag cactgaacga actggatctg
    27781 gaccgtgagt tcctgaaagc cggtggcgtg ttcactgatg aagcgatcga tgcgtacatc
    27841 gctctgcgtc gcgaagaaga tgaccgcgtg cgtatgactc cgcacccggt agagtttgag
    27901 ctgtactaca gcgtctaata gttgaagttg tactacccgg cgcaacaacg ccgggattta
    27961 gttgccgtgg aaactttcag cccatctctg catgggcttt tttctccgtc aattctctga
    28021 tgcttcgcgc tttttatccg taaaaagcta taatgcacta aaatggtgca acctgttcag
    28081 gagactgctt tatggcaaca ggcacgcagc ccgatgctgg gcagatcctc aactcgctga
    28141 ttaacagtat tttgttaatc gatgacaacc tggcgatcca ttacgccaac cccgccgcgc
    28201 aacaactgct cgcccaaagc tcccgcaaat tgtttggtac accgttaccg gaactgttga
    28261 gctacttctc attaaatatc gagctgatgc aagaaagtct ggaggcgggg caaggtttta
    28321 ctgataacga agtgacgctg gtcatcgacg ggcgctcgca tatcctttct gtgacggccc
    28381 agcgtatgcc ggacggcatg atcctgctgg agatggcacc gatggataac cagcgccgct
    28441 taagtcagga acagctacag cacgcccagc aggttgctgc ccgtgattta gtgcgcggcc
    28501 tggcacatga gattaaaaat ccgcttggcg gtttacgtgg cgcggcgcag ctgctcagca
    28561 aagcattacc tgacccgtca ctactcgaat ataccaaagt gattatcgaa caggcggacc
    28621 ggctgcgaaa tctggtcgac cgtctgttgg gaccgcaact gcccggtacg cgcgttaccg
    28681 aaagtattca taaagtagct gaacgcgtgg tgacgctggt gtcgatggaa ctgccggaca
    28741 acgtgcggtt gattcgggat tacgacccca gcctgccgga actggcgcac gacccggatc
    28801 aaattgaaca ggtcttgctg aatattgtgc gcaatgcgct acaggcgctg gggccggagg
    28861 gtggtgaaat cattctgcgt acccgcacag cgtttcaact gaccttacac ggcgagcgct
    28921 atcggctggc ggcgcgaatt gatgtggaag ataacgggcc aggtattccg cctcatttgc
    28981 aggatacgct gttttacccg atggtcagcg gccgcgaagg tggcaccggg cttggcttat
    29041 ccatcgcccg taatttgatt gatcagcatt caggcaaaat tgaatttacc agttggccag
    29101 gtcataccga gttctcggtt tacctgccta tcaggaaata aaggtgacgt ttatgcaacg
    29161 agggatagtc tgggtagtcg atgacgatag ttccatccgt tgggtgcttg aacgtgcgct
    29221 cgctggagcg ggtttaacct gtacaacatt tgagaacggc gcggaggtac tggaggcgct
    29281 ggcgagcaaa acgccggatg tgctgctttc agatatccgt atgccgggaa tggacgggct
    29341 ggcgctgctc aagcagatta aacagcgcca tccgatgctt ccggtcatca ttatgaccgc
    29401 acattccgat ctggatgctg ccgtcagcgc ctatcaacaa ggggcatttg attatctgcc
    29461 caaaccgttt gatatcgacg aagccgtggc gctggttgag cgcgccatca gtcattacca
    29521 ggaacagcag cagccgcgta atgttcagct taacggccca acgaccgata tcatcggtga
    29581 agcgccagcc atgcaggacg tgttccggat tatcggtcgg ctttcgcgtt cttctattag
    29641 cgtgctgatt aacggcgagt ccggcaccgg taaagaactg gtcgctcatg ccctgcatcg
    29701 ccacagtccg cgagccaaag cgccgtttat cgcgctcaat atggcggcta tcccgaagga
    29761 tttgatcgaa tccgaactgt ttggtcacga gaaaggcgca tttaccggcg cgaataccat
    29821 tcgtcagggg cgttttgaac aggctgatgg tggcacatta ttcctcgatg aaattggcga
    29881 tatgccgctg gatgtgcaga cgcgtttgct gcgcgtactg gcagatggtc agttttatcg
    29941 cgttggcggc tatgcgccgg tgaaagtaga tgtgcggatt atcgctgcca ctcaccagaa
    30001 tcttgaacag cgggtgcagg aaggtaagtt ccgtgaggat ctgttccacc gcctgaacgt
    30061 catccgcgtt catctgccgc cactgcgtga gcgtcgggaa gatattcccc gtctggcacg
    30121 ccatttttta caggttgccg cgcgcgaact gggcgtagaa gcgaagttgc tgcatccgga
    30181 aaccgaagcc gcgctgacgc gcctggcgtg gccggggaac gtgcgccagc tggaaaacac
    30241 ctgtcgctgg ctaacggtga tggccgccgg gcaggaagtg ttgattcagg atttgcccgg
    30301 tgaactgttt gaatcaacgg ttgcggagag tacttctcaa atgcaaccgg acagttgggc
    30361 gacactttta gcgcagtggg cagacagagc gctgcgttcc ggtcatcaaa atctgctttc
    30421 cgaagtgcag cctgagctgg agcggacgtt actgacgacc gcgttgcgac atacgcaagg
    30481 gcataaacag gaagcggcgc ggctactcgg ctgggggcgt aacactctga cgcggaagtt
    30541 aaaagagctg gggatggagt gattcacggc ttgtgtgtaa agattgatta ttgagcgcaa
    30601 attgctggtg ttttgcgctt tactgttccg ataagttcag tatgatcttg cccggaaaac
    30661 ggggagagtc attatgctgg aatcaataat taatctggta tcgagtggcg cagttgacag
    30721 ccacacaccg caaactgctg ttgctgccgt gctgtgtgcc gcgatgattg ggctgtttag
    30781 ttgagtttca gattgctgat gaagtgctct ttgttcatgc cggatgcggc gtgaacgcct
    30841 tatccggcct acaaaagcaa tattttcaac agattttgca ggcctgataa gcgtagcgca
    30901 tcaggcaatc ttgttctcag cacattgatg gcgctttcgt tttctacttt gtaaacgaag
    30961 cgccattcac aatttaaatc acccgagaga actgctgcat ccgcgctttc tggcgcagat
    31021 aggtatcaaa gcacatgcaa atgttgcgga tcagcaagcg acctttcgcc gtcacctgta
    31081 ttcccttctc atccacatcc accagcccat cttttgctaa cggggcgagc agcttgagat
    31141 cttcggcaaa gtaatcagcg aagagcaaat cccactgttg ctcaatggga gcgtaatcca
    31201 gacggaagtt gcagatgagc gacttaatca catcgcggcg aatacagtca tcacgcgtta
    31261 gcgcaatacc acgccacagc gcattgcctt gttcatccac ttgctgatag tactgcttca
    31321 actctttctg gttctgcgcg tagcagtcgc caatcatgct gatggcggaa acgcccatcc
    31381 ccagcagatc ggtatcgccc tgagtggtgt agccctggaa gttacgatgc agcacgcctt
    31441 cacgctgggc caccgccagc tcgtcatccg gacgggcaaa gtgatccata ccgataaact
    31501 gatagcccga ttgtgtcagg aaggcgatgg tttcctgcag gatatcgagt ttttgctgcg
    31561 gactcggcag gtcagcatct ttgattttgc gctgagcagc aaaaatggtc ggcagatgcg
    31621 cgtagttaaa gacactcaga cgatcggggt tcagctccgc cacacgtttc agggtaaagg
    31681 cgaaactctc cggcgtctgt ttcggcaggc cgtaaatcag gtcgatgttg gtggaggtaa
    31741 agccaatctc acgcgcatgg ttaagcagtg caaagatgaa ctcttcatcc tgctcgcggt
    31801 taaccagacg ttgcacttct ttgttgaagt cctgcacgcc catgctcagg cgattaaagc
    31861 cttcggcgcg taaatgatcg agtacatcca gttcgatttc ccgcggatcg acttcgatcg
    31921 agatctccgc atcggcattg aactggaagt tttcgcgcag cagcttcatc agacggctga
    31981 tttgcgcttt attcagatac gtcggcgttc cgccgcccca gtgcaattgg ctgacgtgac
    32041 gcccggcgaa cagcggtgca cgatggacga tttcttgctc cagcgcgtcc agatactgat
    32101 cggccttgtg ctgctggcga gtaactatct tattgcaacc gcagaagtaa caaagcttat
    32161 ggcagaacgg aatatgtacg tagagagata atggacgctc aggatagcgc gccacggctt
    32221 gtaaaaacgc ctgttcgccg aagtcttctg aaaactccag cgcggtcggg tacgaggtgt
    32281 atcgtggccc ggaatagtta tatttctgga tcagggccag atcccagtcg atttgctgta
    32341 cagacatgct cactccttcc gatggcgtct ctggcggcta cggcgtaccg gcggatgccc
    32401 gtttcgagtc atcagccggt tgcgcagcca cttttgtcgc cgcgacaacc gctgtagttt
    32461 aacgaataac cacaccagat aacatataac cggaagggtt atcagcagaa cgggaaggcc
    32521 cacggcggca atccgttagt tgccccgcag cagacgcatc atgtcttctt gcttctcgtc
    32581 ttcttcctct tcttcgtcat cgtcataaga gaggccgagt ttctgcatca actcatcaat
    32641 acgatccagt ttggcatcca cccaggattg ctcttcggcg ctcagggttt cgcctgcttc
    32701 cagacgttcc agcagcgcat ccagacgctc atccgtttcc agtaactcca actccgcctg
    32761 cggtgaaagc ataggtttct cactcttcgg tttgtgctgt ttggtgactt tttcagtcac
    32821 gcccaatgga ataggggttt tactgccaat acgtggatct ttcggtgcgt tctggccttt
    32881 gctgcctgac gtggtgttac cgcccgctgc gcggctgccc ggcgcatgac cacgacgttt
    32941 tttctgacgc ttacggtcac gagcttcctg atccagctcc tcgcgtgttt ttcgacgcgc
    33001 ttttgcatga cctttgctgc gtgagtttga agatgatggt ttcataatgg ttcgtctttt
    33061 agccgtttta ttcagtatag atttgcggcg gaatctaaca gaaagcaagc aaagaaaaaa
    33121 ggcgacagat tactctgtcg ccttttttcc tgactcataa cccttaacgg gtcttacaat
    33181 ccttgcaggc cgccaacaca ccctgttttt ccattagccg gaacaatccg tgttgattcc
    33241 atttccgttt aattacgtct ccggacgttt atcttcctga caatcctctg tcttcgcctc
    33301 ctggcgctcc tgaccctcat cctgagtcat tgttcctgtt agcgtcctcg ctctatgggt
    33361 gctactttac gcctttgctt taaataaaca atcaaccgcc atcagtattt gcacattttc
    33421 agagttttat ataaaagcaa ttcactgatt tatatagctt atttgacata cgtcgcagaa
    33481 ttttctctgc tatttcgcat aaagactatc ggcaatgtct cacaaagcat atggctttta
    33541 cttacaacgc tcacgccgaa aaatccgcct tttattatgg ttcaggtata atcgccgcaa
    33601 agctttgact gatggagacg accgctttga ctaatttgaa ttatcaacag acgcattttg
    33661 tgatgagtgc gcctgatatt cgccacctac cttccgatac cggaattgaa gtggcttttg
    33721 caggccgttc caacgcaggt aaatccagcg cgctgaacac gctgactaac cagaaaagcc
    33781 tggctcgtac ctcaaaaacc ccggggcgca cccagcttat caacctgttt gaagtggctg
    33841 acggcaagcg tctggttgac ttgcctgggt acggttatgc ggaagtcccg gaagagatga
    33901 agcgcaaatg gcagcgcgcg ctcggcgagt acctcgaaaa acgtcagagc ctgcaaggtc
    33961 tggtggtgct aatggatatt cgccatccgc tgaaagattt ggatcagcag atgattgagt
    34021 gggcggtaga cagcaatatc gccgtgctgg tactgctgac caaagcggac aaactggcaa
    34081 gcggcgcacg taaagcgcaa ttgaatatgg tgcgtgaagc ggtgctggcg tttaacggtg
    34141 atgtgcaggt tgaaacgttt tcttcgctga agaaacaagg cgtggacaag ctgcggcaga
    34201 aactggatac ctggtttagc gagatgcagc ctgtagaaga aacgcaggac ggcgaataat
    34261 tttcttgcct taatgcttgt gccggatgtg gcgtatcagg cattacggat cttttctttc
    34321 gcccaataaa aaacgcccca gtcattactg actggggcgg ctaaaatatt cagccaaatc
    34381 cgattacgtg aagtaaaagg tctgaaagat agaacatctt acctctgtac cctacgcgac
    34441 taactttact cttttttgtt cagttcagaa agcatttttt gtaatttttt ttcagttgtt
    34501 gcataggaat ttctaatagc catcacaaaa cgtgacagct tatgttgctt acttacgaaa
    34561 aaagggcatg ttcaggcgaa tcttagtgcg cctgatccca gttttcgcca ctccccactt
    34621 ccaccagcag cggcacatcc agacgggtac agttttccat cagttgatga atctgcttcg
    34681 cgacggcatc gacatcatct ttatgaactt caaataccag ttcatcgtgt acctgcatga
    34741 tcatacgcac acgcggttgc tcagcctgta gccacgcatc aacggcaatc atcgcccgtt
    34801 tgataatgtc ggcggcggtt ccctgcattg gcgcgttaat ggctgcacgt tcagccgctg
    34861 cacgacgagc cccattgctg gatttgatat ccggcagata cagacggcgt ccgtccagcg
    34921 tttcaacgta gccctgctct ttcgcctgag cacgggtgcg ttccatatac tccagcacgc
    34981 cagggtagcg ttcgaagtaa aggtccatgt acttctgcgc ttctttacgt ggaatgttca
    35041 attgccgcgc cagaccgaaa gcactcatgc cataaatcag accaaagttg atcgctttcg
    35101 cgctacggcg ttgctcgctg gttacggttt ccagtggcaa accaaacact tctgccgccg
    35161 tagcccggtg gatatctttt ccttccgcga atgcggtcag caagcctttg tcacgcgaga
    35221 gatgcgccat aatgcgcagt tcaatctgcg agtagtccgc cgagacaatc acataatcct
    35281 ctggcgcaat aaacgcctgg cggatacgac gaccttcttc gttacgcacc ggaatgtttt
    35341 gcaggttagg atcggttgac gataaacgtc ccgttgcagt tactgcctgg tgataagagg
    35401 tatgcacacg cccggttttc gggttgatca tcagcggcag cttgtcggtg taagtcgatt
    35461 tcagcttcgc cagaccacga tactccagaa tcacttttgg caacggatag tccagcgcca
    35521 gttcttccag tacctcttcc gacgttgacg gcgcgccacc cggcgttttc ttcagcggtt
    35581 taattccctg tttttcaaac agaatggttt gcaactgctt ggtggaggaa aggttaaatt
    35641 cttcacctgc aatttcatgc gctttctttt ccagctcagc cagacgaaga gtgagctctt
    35701 cagaatgatt gtgcagcact ttcggatcga tcttcacacc gttacgttca atgcgtgaaa
    35761 gcaccggcac tagcggcatt tcgatattct cgaagacgtt caacggccct ttgtgttttt
    35821 gcagatccgg ccacattttc agatgcaact gcaaggtgac atctgcatct tcggcggcgt
    35881 aacgtccggc ctcttcgagg gcaatctggt taaaggtcag ttgatttttg cctttacccg
    35941 caatctcttc aaaagtgatg gttttgtgct tcaaccaacg ttccgcgagg ctgtccatat
    36001 cgtgacgccc ggcaacgcta ttgagaatgt aggactccag catggtatca aacgcaatcc
    36061 cacgcagctc aatgccgtaa ttcgccagaa taccgcgatc gtatttcagg ttttgcccaa
    36121 ccttcagcgc cttttcatct tccagcagcg gttttagcaa ctcgagtgca cgctcgcgag
    36181 agatttgatc gggcgcatca agataatcat gagcaaccgg aatatatgcc gctacgcctg
    36241 gctcgatagc aaaagaaagc ccgaccaggt tagcagagat gttatcaagg ctgtcggttt
    36301 cggtatcaaa tgcaaatacc ggcgcttttt ccagcttcgc aatccacgct ttcagtgttt
    36361 cttcatcaag gatggtgacg tagttgtcat aagaaatcac cgttgccgtc acttctggcg
    36421 cttcgtctac aacactggtt tcctgcggct tcgcggctgg ttttgcccct ttggcctgta
    36481 accatttgcc cgcttcgaca tcagcagtcc agcgtttgaa ctcatacttt ttgaacagcc
    36541 ctaacaactc ttctgccacc ggtggctgca cttccagttg ttcacaggtc agctccagtt
    36601 caacgtcggt tttaatcgtc gccagctggt aagagagata agccacttct ttgttttgct
    36661 cgagcttcgc cgccattgtt ttcgcgccac ggaagctcaa cccagcaatt ttttctggct
    36721 cggcatacag cgtatccagt ccgccaagac cttgtagcaa tgcttgcgcg gttttttcac
    36781 cgacgcccgg tacgccaggg atgttatcag aggagtcacc catcagcgcc aggaagtcga
    36841 tgatcagttc tggcggcacg ccgtacttat tcaccacctc ttccgggccg aggatggtat
    36901 tcgtcatggt attgataagc gtaatatttg gcgtcaccag ctgcgccata tctttatcgc
    36961 ctgtgcttat cagcaccgga cgcccggctt tttcggcttc ccgcgccaga gtaccgataa
    37021 cgtcgtccgc ttctacgcca gaaaccgcca gcagcggcag tcccatcgct ttaaccatcg
    37081 cgtgcaaggg ttcgatttgt gcacgcagat cgtccggcat tggcgggcga tgtgatttgt
    37141 aatgttcaaa cagttcatca cgaaaggttt ttcccttggc gtcaaagacc accgctgcat
    37201 gcgtcggttt atattgcatg attagactgc gcagcatgtt gaggacacca tacatcgcac
    37261 cggtcggctc gcctgcgctg ttagtcagcg ggggaaacgc gtgatatgcg cgataaagat
    37321 aagatgaacc atctacaagg ataagtggat tttgggggat ctgaaccata atgtccgtgc
    37381 ctgtttatca gattatgggt aaaggatgcc acagacagga tgaaaacatg agtttttcac
    37441 cgcttttcgt gaaaatcttt tgtagatctt ctggatcgct cgcaaatcgt catgtggata
    37501 actttgtgca taaattattt acatcgcttt taatttatta tcatgctttt tacagcatga
    37561 tatttgtctt tgcttttcat acagttaaat ataaagccat ccctaataat tgtgataatg
    37621 ggattttttc taatgtggat atatatgaat tatttttctc cttaaggatc atccgttatt
    37681 tggggcgttt tctgctaaaa tcggcgcctt gcgcttcttt agcgcactgt ctatggctaa
    37741 ccttttgaat aaattcatta tgacgagaat actcgctgcg ataacccttt tgctgagtat
    37801 cgtattgacc attttggtca ctattttttg ctccgtcccg atcatcattg ccgggattgt
    37861 aaaactattg ctacctgtgc cagtcatttg gcgaaaggtt tcgcgttttt gtgattttat
    37921 gatgtattgc tggtgtgaaa gtctggcggt attactgcat ctgaacccac acctgcaatg
    37981 ggaagttcac gggctggaag ggttaagtaa gaagaactgg tatctgctta tttgtaacca
    38041 ccgtagttgg gcagatattg tcgtactgtg cgtgctgttt cgtaagcaca ttccgatgaa
    38101 taaatatttc ctcaagcagc agcttgcctg ggtgccattt cttggcctgg catgctgggc
    38161 gctggatatg ccgtttatga agcgttattc ccgcgcttat ttattacgtc atccggaacg
    38221 acgtggtaaa gatgttgaaa ccacccggcg ttcttgtgaa aagtttcgcc tgcaccccac
    38281 caccattgtc aatttcgttg aaggctcccg tttcacgcaa gaaaaacatc agcaaacgca
    38341 ctccactttt caaaacttgt tgccaccaaa ggcggcaggc attgcgatgg cgctaaatgt
    38401 actgggtaaa caattcgata aactgcttaa tgttacgctg tgttatccgg acaataaccg
    38461 tcagccgttc ttcgatatgt taagcggtaa actgacgcga attgtcgtac atgtggattt
    38521 gcagcctatt tccgatgagt tacatggcga ctacatcaat gacaaaagct ttaagcgcca
    38581 ttttcagcaa tggctgaact cactgtggca ggaaaaagac cggcttctga cgtcgttgat
    38641 gtcatcacag cggcaggaca agtaaatttt gctcgcctct gaatgagcag aggcgagtga
    38701 gtattttaat gaacaaaacg tccggcacga gacataaatt cttcttcgct catctcctgt
    38761 ccgttaaaaa caactttacc cggtgtataa cgcagttgca gcgaggcggt attgttgtca
    38821 atcgtgatta actgaaacat ctgacctaat gtcatcatcc cgctgatttg tttatcagcc
    38881 cgcttttgcg ctttttcagc atccattcct tcagataaat taagctgttt tgcggtttct
    38941 gtgacaacat taagcggtaa cttcacatca aaaatgagcg atttaatatc tttgtttgtg
    39001 gatgatgagg atttggctgg gtcggcaata ctgatatcca gattggcatt tagttcgccg
    39061 agtgcattct tccatcttac cggttgttta atggtcggct cacttttttg taataacggt
    39121 aaatactctt tgaacaatgc agcattcact tcttgcagag caacttcatc gtttgctaac
    39181 tcagggtgtg cagcaagctg cttctgcatc gcaatgttat actggatgac aaattggcgc
    39241 accgctgagg gatcaataga ttcagcaatc aatgaccact caccactacc gagttgctga
    39301 ttcccttttg ttaacttatc aagttcgtag gtcagattga cattgatata atctttgtct
    39361 ttaacgcgat ccagcctggt ttttgcaacg aaagcatcga tttgggcaac gtcttctccc
    39421 tgattgataa tgttaatctg attgaatttc gactcgcttt cgccaaccgg aaatttcttt
    39481 tcttctaatc gcgttagaga atcaatggta aaacttttcg ccgttacttt attcaatggc
    39541 gataattcca gaacaagatc ttcgactttg ccttcaatat ccagattaga caatgaacta
    39601 ccatcaccat taaaagtaaa ctggccctca ccaaaagaaa actttccata ttttgcaggg
    39661 gcaacatcta atgtcgtgga gaatgagcca ccaaacgcga atcgcaatgt cgcttccata
    39721 ggagatttat tttttgcggc gataaatagc ggttgtgtca gttcattatc cactaatttc
    39781 acttttgccg ctgccagtgc ggggatgaca ttaccacgca ttaacattgt gatgggtaac
    39841 ggaccgtgct caacatccac atcaaacgca actttctggc caggtttgat attgagatca
    39901 ggtgcaccgt tatcaaatgt catttgcatt tgaaaacgag aattaaaaaa gcctttgtca
    39961 aaatttttat aacttaacgt catatcgtag gcatgttcac ctttcttttt agcatcgtta
    40021 aaatctttaa taaacttttc gataccaggc tgaatttgcg tacctgtgta ccatgtgcca
    40081 ccaccccaga ttacggctaa cgcaacaata acacctgtag ctgacttacg tatcatataa
    40141 atattccctg ataaaacagc aaaactttga atatccactt atgctgaaat aatgtccagc
    40201 g
//
LOCUS       DAZGQL010000037        40043 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.037, whole genome shotgun sequence.
ACCESSION   DAZGQL010000037 DAZGQL010000000
VERSION     DAZGQL010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40043
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.037"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..1064)
                     /locus_tag="ACHKIR_002222"
     CDS             complement(<1..1064)
                     /locus_tag="ACHKIR_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO9989960.1"
                     /translation="MKKAIAYMRFSSPGQMSGDSLNRQRRLIAEWLKVNSDYYLDTIT
                     YEDLGLSAFKGKHAQSGAFSEFLDAIEHGYILPGTTLLVESLDRLSREKVGEAIERLK
                     LILNHGIDVITLCDNTVYNIDSLNEPYSLIKAILIAQRANEESEIKSSRVKLSWKKKR
                     QDALESGTIMTASCPRWLSLDDKRTAFVPDPDRVKTIELIFKLRMERRSLNAIAKYLN
                     DHAVKNFSGKESAWGPSVIEKLLANKALIGICVPSYRARGKGISEIAGYYPRVISDDL
                     FYAVQEIRLAPFGISNSSKNPMLINLLRTVMKCEACGNTMIVHAVSGSLHGYYVCPMR
                     RLHRCDRPSIKRDLVDYNII"
     gene            complement(1122..1271)
                     /gene="ylcJ"
                     /locus_tag="ACHKIR_002223"
     CDS             complement(1122..1271)
                     /gene="ylcJ"
                     /locus_tag="ACHKIR_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001299444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YlcJ"
                     /protein_id="HFO9989961.1"
                     /translation="MSLVLCFLLMSLFFMYSFVLSRLWRKKIAIRLLLYIQDNVTLIV
                     FLNKN"
     gene            complement(1319..1651)
                     /gene="emrE"
                     /locus_tag="ACHKIR_002224"
     CDS             complement(1319..1651)
                     /gene="emrE"
                     /locus_tag="ACHKIR_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter EmrE"
                     /protein_id="HFO9989962.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSTPH"
     gene            complement(1719..2021)
                     /locus_tag="ACHKIR_002225"
     CDS             complement(1719..2021)
                     /locus_tag="ACHKIR_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ren"
                     /protein_id="HFO9989963.1"
                     /translation="MTGKEAIIHYLGTHKSFCAQDVAAVTGATVTSINQAAAKMARAG
                     ILVVDGKVWRTVYYRFATREEREGKVSTNLIFKECRQSAAMKRVLRVYKRTSMGTQ"
     gene            complement(2018..2719)
                     /locus_tag="ACHKIR_002226"
     CDS             complement(2018..2719)
                     /locus_tag="ACHKIR_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFO9989964.1"
                     /translation="MKNIAAQMINFDREQMRRIANNMPEQYDEKPQVQQVAQIINGVF
                     SQLLATFPASLANRDQNELNEIRRQWVLAFRENGITTMEQVSAGMRVARRQNRPFLPS
                     PGQFVAWCREEASVTAGLPNVSELVDMVYEYCRKRGLYPDAESYPWKSNAHYWLVTNL
                     YQNMRANALTDAELRRKAADELSCMTARINRGEAIPEPVKQLPVMGGRPLNRAQALAK
                     IAEIKAKFGLKGASV"
     gene            complement(2716..3735)
                     /locus_tag="ACHKIR_002227"
     CDS             complement(2716..3735)
                     /locus_tag="ACHKIR_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFO9989965.1"
                     /translation="MTGSEMAKAGLLEQNRLSGANRNTLIAGGIMANTAEIFNFPVPD
                     AAQKEPRVADLDDGYTRIANELLEAVMLAGLTQHQLLVFLAVMRKTYGFNKKLDWVSN
                     EQLSELTGILPHKCSAAKSVLVKRGIFIQSGRNTGINNVVSEWSTLPESGKKNKVYLK
                     EVNLPESGKKSLPKSGKGVYPNQVNTKDKLTKDNIKPFSSENSGESSDQPENDLPVVK
                     PDAAIQSGSKWGTAEDLTAAEWMFDMVKTIAPSARKPNFAGWANDIRLMRERDGRNHR
                     DMCVLFRWACQDNFWSGNVLSPAKLRDKWTQLEINRNKQQAGVTASKPKLDLTNTDWI
                     YGVDL"
     gene            complement(3732..4271)
                     /locus_tag="ACHKIR_002228"
     CDS             complement(3732..4271)
                     /locus_tag="ACHKIR_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT domain-containing protein"
                     /protein_id="HFO9989966.1"
                     /translation="MQPLTYQQTSGFSPTAVINRSQTKQVPGHEKIRDAVRAWSAVDN
                     QDVVATLIVNEYREQGGGTIDFPDDVSRARQKLFRFLDNKFDSEKYRNNVRELTPAIL
                     AVLPLEYRGYLVEQDSFMARLAEMEKELSEAKQAVILNAPRHQKLKEISEGIVSMFRV
                     DPDLAGPLMAMVTTMLGAI"
     gene            complement(4341..4571)
                     /locus_tag="ACHKIR_002229"
     CDS             complement(4341..4571)
                     /locus_tag="ACHKIR_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO9989967.1"
                     /translation="MNPAIKTAINIVGSQKKLGAACEVSQQAVYKWLHNKAKVSPEHV
                     GSIVTATGGVVKAYQIRPDLPKLFPHTEKNAA"
     gene            4676..5365
                     /locus_tag="ACHKIR_002230"
     CDS             4676..5365
                     /locus_tag="ACHKIR_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFO9989968.1"
                     /translation="MKTTLSERLKEARLARGLTQKALGDLVGVSQAAIQKIETGKANQ
                     TTKIVEIANALGVRAEWLSSGVGNMSDSTVQPIQSTVSHSKYFKIDVLDIEVSAGPGV
                     INREFVEVLRSVEYSFDDARHMFDGRKAENIRIINVRGDSMSGTIEPGDLLFVDITVK
                     SFDGDGIYAFLYDDTAHVKRLQMMKDKLLVISDNKSYSPWDPIEKDEMNRVFIFGKVI
                     GSMPQTYRKHG"
     gene            5961..6167
                     /locus_tag="ACHKIR_002231"
     CDS             5961..6167
                     /locus_tag="ACHKIR_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor protein"
                     /protein_id="HFO9989969.1"
                     /translation="MVHQHYGTQTVNRGAVMPGMLVKHKDGTWTASANLRGRLYLHRG
                     IERTYTRDLLVEVFLDGRGNGLNH"
     gene            6243..6539
                     /locus_tag="ACHKIR_002232"
     CDS             6243..6539
                     /locus_tag="ACHKIR_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0060703 - deoxyribonuclease inhibitor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFO9989970.1"
                     /translation="MNAYYIQDRLEAQSWARHYQQIAREEKEAELADDMEKGLPQHLF
                     ESLCIDHLQRHGASKKAITRAFDDDVEFQERMAEHIQYMVETIAHHQVDIDSEV"
     gene            6545..7330
                     /gene="bet"
                     /locus_tag="ACHKIR_002233"
     CDS             6545..7330
                     /gene="bet"
                     /locus_tag="ACHKIR_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HFO9989971.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQF
                     IALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNE
                     SCTCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCA
                     RLAFGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDD
                     DLLPLCSQIFRRDIHASSELTQAEAVKALGFLKQKATEQKVAA"
     gene            7327..8007
                     /locus_tag="ACHKIR_002234"
     CDS             7327..8007
                     /locus_tag="ACHKIR_002234"
                     /EC_number="3.1.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lambda exonuclease family protein"
                     /protein_id="HFO9989972.1"
                     /translation="MIPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNITE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQY
                     SMWVTRKDAWYFANYDPRMKREGLHYVVIERNEKYMASFDEMVPEFIEKMDEALAEIG
                     FVFGEQWR"
     gene            8004..8186
                     /locus_tag="ACHKIR_002235"
     CDS             8004..8186
                     /locus_tag="ACHKIR_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 domain-containing protein"
                     /protein_id="HFO9989973.1"
                     /translation="MTHPHDNIRVGAITFVYSVTKRGWVFPGLSVIRNPLKAQRLAEK
                     INNKREAVCTKHLLLS"
     gene            8159..8350
                     /locus_tag="ACHKIR_002236"
     CDS             8159..8350
                     /locus_tag="ACHKIR_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HFO9989974.1"
                     /translation="MHKASPVELRTSIEVAHSLAQIGVRFVPIPVETDEEFHTLATSL
                     SQKLEMMVAKAEADERDQV"
     gene            8427..8642
                     /locus_tag="ACHKIR_002237"
     CDS             8427..8642
                     /locus_tag="ACHKIR_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFO9989975.1"
                     /translation="MTPQQENALRSIARQANSEIKKARQHFPDKNVDDICRSVLKKHR
                     ETVTLMGFTPTHLSLAIGMLNGVFKER"
     gene            8741..8950
                     /locus_tag="ACHKIR_002238"
     CDS             8741..8950
                     /locus_tag="ACHKIR_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFO9989976.1"
                     /translation="MADIIDSASEIEELQRNTAIKMRRLNYQTVSATHCCECGDPIDE
                     RRRLAVQGCRTCASCQEEIELKNKQ"
     gene            9007..9285
                     /locus_tag="ACHKIR_002239"
     CDS             9007..9285
                     /locus_tag="ACHKIR_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO9989977.1"
                     /translation="MFRIIFPNTWYVDHHGTPCKILRSTHNKVHYIRKGRTCIASMFR
                     FNHDFEPVNKADADRIAEEIETAEHIKKLRDMRSKSRGNHGIIQPHTR"
     gene            9257..9322
                     /locus_tag="ACHKIR_002240"
                     /pseudo
     CDS             9257..9322
                     /locus_tag="ACHKIR_002240"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            9484..10647
                     /gene="intD"
                     /locus_tag="ACHKIR_002241"
     CDS             9484..10647
                     /gene="intD"
                     /locus_tag="ACHKIR_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFO9989978.1"
                     /translation="MSLFRRNEIWYASYSLPGEKRIKESLGTKDKRQAQELHDKRKAE
                     LWRVEKLGDLPDVTFEEACLRWLEEKADKKSLDSDKSRIEFWLEHFEGIRLKDISEAK
                     IYSAVSRMHNRKTKEIWKQKVQAAIRKGKEPPVYEPKPVSTQTKAKHLAMIKAILRAA
                     ERDWKWLEKAPVIKIPAVRNKRVRWLEKEEAKRLIDECPEPLKSVVKFALATGLRKSN
                     IINLEWQQIDMQRRVAWVNPEESKSNRAIGVALNDTACKVLRDQIGKHHKWVFVHTKA
                     AKRADGTSTPAVRKMRIDSKTSWLSACRRAGIEDFRFHDLRHTWASWLIQSGVPLSVL
                     QEMGGWESIEMVRRYAHLAPNHLTEHARKIDDIFGDNVPNMSHSGIMEDIKKA"
     gene            complement(10663..10739)
                     /locus_tag="ACHKIR_002242"
     tRNA            complement(10663..10739)
                     /locus_tag="ACHKIR_002242"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10703..10705),aa:Arg,seq:tct)
     gene            11010..11876
                     /gene="folD"
                     /locus_tag="ACHKIR_002243"
     CDS             11010..11876
                     /gene="folD"
                     /locus_tag="ACHKIR_002243"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HFO9989979.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            11878..12090
                     /gene="ybcJ"
                     /locus_tag="ACHKIR_002244"
     CDS             11878..12090
                     /gene="ybcJ"
                     /locus_tag="ACHKIR_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HFO9989980.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            12198..12719
                     /gene="ybcI"
                     /locus_tag="ACHKIR_002245"
     CDS             12198..12719
                     /gene="ybcI"
                     /locus_tag="ACHKIR_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO9989981.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKVIPPRLLFAGIVLAMLPDADVLS
                     FKFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTPYGHKVIISELMWVWLP
                     GMLLMGMLWWRRR"
     gene            complement(12755..14140)
                     /gene="cysS"
                     /locus_tag="ACHKIR_002246"
     CDS             complement(12755..14140)
                     /gene="cysS"
                     /locus_tag="ACHKIR_002246"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFO9989982.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMNDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            14314..14808
                     /gene="ppiB"
                     /locus_tag="ACHKIR_002247"
     CDS             14314..14808
                     /gene="ppiB"
                     /locus_tag="ACHKIR_002247"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HFO9989983.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKTTKDPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            14811..15533
                     /gene="lpxH"
                     /locus_tag="ACHKIR_002248"
     CDS             14811..15533
                     /gene="lpxH"
                     /locus_tag="ACHKIR_002248"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HFO9989984.1"
                     /translation="MATLFIADLHLCAEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQTLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            15651..16160
                     /gene="purE"
                     /locus_tag="ACHKIR_002249"
     CDS             15651..16160
                     /gene="purE"
                     /locus_tag="ACHKIR_002249"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFO9989985.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGAA"
     gene            16157..17224
                     /gene="purK"
                     /locus_tag="ACHKIR_002250"
     CDS             16157..17224
                     /gene="purK"
                     /locus_tag="ACHKIR_002250"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFO9989986.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPVQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWRLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NSPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVMWAQSKFS"
     gene            complement(17557..18450)
                     /gene="ybcF"
                     /locus_tag="ACHKIR_002251"
     CDS             complement(17557..18450)
                     /gene="ybcF"
                     /locus_tag="ACHKIR_002251"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFO9989987.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTKDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(18447..19262)
                     /gene="ylbF"
                     /locus_tag="ACHKIR_002252"
     CDS             complement(18447..19262)
                     /gene="ylbF"
                     /locus_tag="ACHKIR_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFO9989988.1"
                     /translation="MTIIHPLLASSSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVMAQGIRLGRFTVKQPQRYCLLRITPP
                     AHPQPLAAAWMQRAEETGLFGPLALAAGDPLPAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLSGMLLAAWYYGALDARSGRPFFACSDNLQLVTTAVSVSYLRYAAQ
                     GYFASPLLHFIHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKS"
     gene            complement(19273..20532)
                     /gene="ylbE"
                     /locus_tag="ACHKIR_002253"
     CDS             complement(19273..20532)
                     /gene="ylbE"
                     /locus_tag="ACHKIR_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFO9989989.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAMLDQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPHIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIFLERNMQLQIPGWDFQGACLGLDIRRVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            complement(20542..22209)
                     /gene="fdrA"
                     /locus_tag="ACHKIR_002254"
     CDS             complement(20542..22209)
                     /gene="fdrA"
                     /locus_tag="ACHKIR_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFO9989990.1"
                     /translation="MIHAFIKKGCFQDSVSLMIISRKLSESENVDDVSVMMGTPANKA
                     LLDTTGFWHDDFNNATPNDICVAIRSEAADAGIAQAIMQQLEEALKQLAQGSASSQAL
                     TQVRRWDSACQKLPDANLALISVAGEYAAELANQALDRNLNVMMFSDNVTLEDEIQLK
                     TRAREKGLLVMGPDCGTSMIAGTPLAFANVIPEGNIGVIGASGTGIQELCSQIALAGE
                     GITHAIGLGGRDLSREVGGISALTALEMLSADEKSEVLAFVSKPPAEAVRLKIVNAMK
                     ATGKPTVALFLGYTPAVARDENVWFASSLDEAARLACLLSRVTARRNAIAPVSSGFIC
                     GLYTGGTLAAEAAGLLAGHLGVEADDTHQHGMMLDADGHQILDLGDDFYTVGRPHPMI
                     DPTLRNLLIADLGAKPQVRVLLLDVVIGFGATADPAASLVSAWQKACAARSDSQPLYA
                     IATVTGTERDPQCRSQQIATLEDAGIAVVSSLPEATLLAAALIHPLSPATQPHTPSLL
                     ENVAVINIGLRSFALELQSASKPVVHYQWSPVAGGNKKLVRLLERLQ"
     gene            22526..23575
                     /gene="allD"
                     /locus_tag="ACHKIR_002255"
     CDS             22526..23575
                     /gene="allD"
                     /locus_tag="ACHKIR_002255"
                     /EC_number="1.1.1.154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate dehydrogenase"
                     /protein_id="HFO9989991.1"
                     /translation="MKISRETLHQLIENKLCQAGLKREHAATVAEVLVYADARGIHSH
                     GAVRVEYYAERISKGGTNREPEFRLEETGPCSAILHADNAAGQVAAKMGMEHAIKTAQ
                     QNGVAVVGISRMGHSGAISYFVQQAARAGLIGISMCQSDPMVVPFGGAEIYYGTNPLA
                     FAAPGEGDEILTFDMATTVQAWGKVLDARSRNMSIPDTWAVDKNGAPTTDPFAVNALL
                     PAAGPKGYGLMMMIDVLSGVLLGLPFGRQVSSMYDDLHAGRNLGQLHIVINPNFFSSS
                     ELFRQHLSQTMRELNAITPAPGFNQVYYPGQDQDIKQRKAAVEGIEIVDDIYQYLISD
                     ALYNTSYETKNPFAQ"
     gene            23597..24832
                     /gene="allC"
                     /locus_tag="ACHKIR_002256"
     CDS             23597..24832
                     /gene="allC"
                     /locus_tag="ACHKIR_002256"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047652 - allantoate deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA];
                     GO_process: GO:0009442 - allantoin assimilation pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HFO9989992.1"
                     /translation="MITHFRQAIEETLPWLSSFGADPAGGMTRLLYSPEWLETQQQFK
                     KRMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWL
                     AIDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNS
                     FVDAMKACGFTLPNAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTV
                     TLNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNV
                     VPGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKEL
                     VATLTELCESEKQNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITD
                     LAEGVKTLALMLYQLAWQK"
     gene            24843..25628
                     /gene="allE"
                     /locus_tag="ACHKIR_002257"
     CDS             24843..25628
                     /gene="allE"
                     /locus_tag="ACHKIR_002257"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071522 - ureidoglycine aminohydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0010136 - ureide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HFO9989993.1"
                     /translation="MGYLNNVTGYRDDLLANRAIVKHGNFALLTPDGLVKNIIPGFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYIPVEGHAPWLVSGNASELEHIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNN
                     WIPVKKGDYIFMGAYSLQAGYGVGRGEAFSYIYSKDCNRDVEI"
     gene            complement(25757..26902)
                     /gene="glxK"
                     /locus_tag="ACHKIR_002258"
     CDS             complement(25757..26902)
                     /gene="glxK"
                     /locus_tag="ACHKIR_002258"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 3-kinase"
                     /protein_id="HFO9989994.1"
                     /translation="MKIVIAPDSFKESLSAEKCCQAIKAGFSTLFPDAHYICLPIADG
                     GEGTVEAMVAATDGNIVKLEVCGPMGEKVNAFYGITGDGKTAVIEMAAASGLMLVAPE
                     KRNPLLASSFGTGELIRHALDNGIRHIILGIGGSATVDGGMGMAQALGVRFLDAYGQV
                     LAANGGNLARVASIEMDECDPRLANCHIEVACDVDNPLVGARGAAAVFGPQKGATPEM
                     VEELEQGLQNYARVLQQQTEINVCQMAGGGAAGGMGIAAAVFLNADIKPGIEIVLNAV
                     NLAQAVQGAALVITGEGRIDSQTAGGKAPLGVASVAKQFNVPVIGIAGVLGDGVEVVH
                     QYGIDAVFSILPRLAPLAEVLASGETNLFNSARNIACAIKIGQGIKN"
     gene            complement(26924..28225)
                     /gene="ybbY"
                     /locus_tag="ACHKIR_002259"
     CDS             complement(26924..28225)
                     /gene="ybbY"
                     /locus_tag="ACHKIR_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HFO9989995.1"
                     /translation="MFNFAVSRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTMLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IADPNFKIQLPPFALSVAVMCLVLAMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSH
                     SLSGELHWQWFPLGSGGALSPGIILTAVITGLVNISNTYGAIRGTDVFYPQQGAGNTR
                     YRRSFVATGFMTLITVPLAVIPFSPFVSSIGLLTQTSDYTRRSFIYGSVICLLVALVP
                     ALTRLFCSIPLPVSSAVMLVSYLPLLFSALVFSQQITFTARNIYRLALPLFVGIFLMA
                     LPPVYLQDLPLTLRPLLSNGLLVGILLAVLMDNLIPWERIE"
     gene            complement(28282..29643)
                     /gene="allB"
                     /locus_tag="ACHKIR_002260"
     CDS             complement(28282..29643)
                     /gene="allB"
                     /locus_tag="ACHKIR_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HFO9989996.1"
                     /translation="MSFDLIIKNGTVILENEARVVDIAVKGGKIAAIGQDLGDAKEVM
                     DASGLVVSPGMVDAHTHISEPGRSHWEGYETGTRAAAKGGITTMIEMPLNQLPATVDR
                     ASIELKFDAAKGKLTIDAAQLGGLVSYNIDRLHELDEVGVVGFKCFVATCGDRGIDND
                     FRDVNDWQFFKGAQKLGELGQPVLVHCENALICDELGEEAKREGRVTAHDYVASRPVF
                     TEVEAIRRVLYLAKVAGCRLHVCHISSPEGVEEVTRARQEGQDVTCESCPHYFVLDTD
                     QFEEIGTLAKCSPPIRDLENQKGMWEKLFNGEIDCLVSDHSPCPPEMKAGNIMKAWGG
                     IAGLQSCMDVMFDEAVQKRGMSLPMFGKLMATNAADIFGLQQKGRIAPGKDADFVFIQ
                     PNSSYVLTNDDLEYRHKVSPYVGRTIGARITKTILRGDVIYDIEQGFPVAPKGQFILK
                     HQQ"
     gene            complement(29704..31158)
                     /gene="ybbW"
                     /locus_tag="ACHKIR_002261"
     CDS             complement(29704..31158)
                     /gene="ybbW"
                     /locus_tag="ACHKIR_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative allantoin permease"
                     /protein_id="HFO9989997.1"
                     /translation="MEHQRKLFQQRGYSEDLLPKTQSQRTWKTFNYFTLWMGSVHNVP
                     NYVMVGGFFILGLSTFSIMLAIILSAFFIAAVMVLNGAAGSKYGVPFAMILRASYGVR
                     GALFPGLLRGGIAAIMWFGLQCYAGSLACLILIGKIWPGFLTLGGDFTLLGLSLPGLI
                     TFLLFWLVNVGIGFGGGKVLNKFTAILNPCIYIVFGGMAIWAISLVGIGPIFDYIPGG
                     IQKAENSGFLFLVVINAVVAVWAAPAVSASDFTQNAHSFREQALGQTLGLVVAYILFA
                     VAGVCIIAGASIHYGADTWNVLDIVQRWDSLFASFFAVLVILMTTISTNATGNIIPAG
                     YQIAAIAPTKLTYKNGVLIASIISLVICPWKLMENQDSIYLFLDIIGGMLGPVIGVMM
                     AHYFMVMRGQINLDELYTAAGDFKYYDNGFNLTAFSVTLVAVILSLGGKFIPFMEPLS
                     RVSWFVGVIVAFAAYALLKKRTAAEKTGEQKVTG"
     gene            complement(31328..32206)
                     /gene="glxR"
                     /locus_tag="ACHKIR_002262"
     CDS             complement(31328..32206)
                     /gene="glxR"
                     /locus_tag="ACHKIR_002262"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFO9989998.1"
                     /translation="MKLGFIGLGIMGTPMAINLARAGHQLHVTTIGPVADELLSLGAV
                     SVETARQVTDASDIIFIMVPDTPQVEDVLFGENGCTKASLKGKTIVDMSSISPIETKR
                     FARQVNELGGDYLDAPVSGGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVG
                     GNGDGQTCKVANQIIVALNIEAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHG
                     ERMIKRTFNPGFKIALHQKDLNLALQSAKALALNLPNTATCQELFNTCAANGGSQLDH
                     SALVQALELMANHKLA"
     gene            complement(32306..33082)
                     /gene="hyi"
                     /locus_tag="ACHKIR_002263"
     CDS             complement(32306..33082)
                     /gene="hyi"
                     /locus_tag="ACHKIR_002263"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HFO9989999.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKI
                     NCLVGKTPTGFSSEQIHTTLVENLRYAANMLMKEDILLLIEPINHFDIPGFHFTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGE
                     INYEYLFTVIENSDYNGWVGCEYKPQTTTEAGLRWMDPYR"
     gene            complement(33095..34876)
                     /gene="gcl"
                     /locus_tag="ACHKIR_002264"
     CDS             complement(33095..34876)
                     /gene="gcl"
                     /locus_tag="ACHKIR_002264"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009028 - tartronate-semialdehyde synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HFO9990000.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGASHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMSVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSIPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPERNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKILMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTEACFMHYE"
     gene            complement(34966..35781)
                     /gene="allR"
                     /locus_tag="ACHKIR_002265"
     CDS             complement(34966..35781)
                     /gene="allR"
                     /locus_tag="ACHKIR_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HFO9990001.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            complement(35859..36341)
                     /gene="allA"
                     /locus_tag="ACHKIR_002266"
     CDS             complement(35859..36341)
                     /gene="allA"
                     /locus_tag="ACHKIR_002266"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HFO9990002.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            36571..37497
                     /gene="allS"
                     /locus_tag="ACHKIR_002267"
     CDS             36571..37497
                     /gene="allS"
                     /locus_tag="ACHKIR_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HFO9990003.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLCQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAIEDIVTLFTQRRPEISGFLEIFGNPRS"
     gene            37566..38660
                     /gene="mnmH"
                     /locus_tag="ACHKIR_002268"
     CDS             37566..38660
                     /gene="mnmH"
                     /locus_tag="ACHKIR_002268"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HFO9990004.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQ
                     RSHIVQSWLHAAGIDYPLVKGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQNLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAARLDAALTTQLTTGSTDGHLAWLVPLLEEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKTR"
     gene            38776..39141
                     /gene="ylbG"
                     /locus_tag="ACHKIR_002269"
                     /pseudo
     CDS             38776..39141
                     /gene="ylbG"
                     /locus_tag="ACHKIR_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014543172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(39908..40043)
                     /locus_tag="ACHKIR_002270"
                     /pseudo
     CDS             complement(39908..40043)
                     /locus_tag="ACHKIR_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308588.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT        10198 a        10471 c         9732 g         9642 t
ORIGIN      
        1 ttaatgatat tataatcaac caaatctctt tttattgatg gcctgtcaca tcgatgtaat
       61 cttctcatcg gacaaacata atagccatgc aaacttccag ataccgcatg aacaatcatg
      121 gtattaccac aagcctcaca cttcataact gttcgaagta gatttattag cataggattc
      181 ttgctactat tgctaatacc aaaaggtgcc aaccgaattt cctgtacagc gtaaaacaaa
      241 tcatctgata tgactctggg ataatagcca gcgatttcac ttatcccttt ccctcttgca
      301 cgatatgaag gtacgcatat acctatcaga gctttattcg ctaataattt ttcaattaca
      361 gaaggtcccc atgcactttc ttttcctgag aaattcttta cagcatgatc atttaaatac
      421 ttggctattg cattcaatga gcgcctttcc atcctgagtt taaaaattag ctcaatagtt
      481 ttcaccctgt cggggtctgg aacaaaagcc gttcttttgt catctaagga gagccatctc
      541 ggacaagacg ccgtcataat cgtacctgat tccagtgcat cctgccgttt tttcttccat
      601 gataatttaa cccgacttga ctttatctcg ctttcttcat ttgccctttg tgctataagt
      661 atggctttta ttaatgaata tggctcattc aaagagtcaa tattatagac tgtattatcg
      721 caaagagtta taacatcaat accgtgattc aaaatcaatt tcagacgttc aatcgcttca
      781 ccgacttttt ctcttgaaag tctgtccaga ctttcaacta acaatgtagt tcctggcaat
      841 atataaccat gctctatagc atctaaaaat tccgaaaaag ctcctgattg tgcatgcttt
      901 cctttgaatg cacttaatcc taaatcttca tatgttatgg tatcaagata ataatcacta
      961 tttaccttta accattcagc aataagtctt ctctgtcggt ttaatgagtc gccagacatc
     1021 tgacctggtg atgaaaatcg catatatgct atggcttttt tcatggtgac acctgctaac
     1081 gtatgctttt ataaacctta gtggtgggat ataatttttg tttaattttt atttaaaaag
     1141 acaattaagg tcacattatc ttgaatatac aacaataatc gtattgcaat tttcttacgc
     1201 cataatcttg aaagcacaaa agaatacata aaaaataaag acattaacaa aaagcataaa
     1261 acgaggctca tataaatata agagcctcca tattttagtc gtttagaaac aaattatttt
     1321 aatgtggtgt gcttcgtgac aataaattaa taaccaacac accggcacaa atcaacatca
     1381 tgcctataat ggctggcagg tccagccgtt ggccgaaaaa tccccatgac agtaagctaa
     1441 tcaggacaat accgactcct gaccagatag cataagcaat ccctgtagga atataagcca
     1501 gcgtctgagc taataaccag aatgatgcac aataacaaat aattgtacca acagatggcc
     1561 ataaccgtgt aaaaccttct gaaaacttca ttaaggttgt accaatgacc tctgcaagta
     1621 ttgcaccacc aagataaata taagggttca tagcatattc tttcctgttc aaactggaga
     1681 gaattgtact acagtttgaa ctcaactcac ctgtttcatc attgtgttcc cattgatgtt
     1741 cttttatata ccctcagtac ccgtttcatc gcggcactct ggcgacactc cttaaaaatc
     1801 agattcgtgc tcacctttcc ttcccgttct tctctggtag cgaaccggta atacaccgtt
     1861 cgccagacct taccatcaac gaccaggatt cctgcccgcg ccattttagc cgcagcctga
     1921 tttatgctgg ttacggttgc gcctgttacc gcggcaacgt cctgtgcaca gaagctctta
     1981 tgcgtcccca ggtaatgaat aattgcctct ttgcccgtca tacacttgct cctttcagtc
     2041 cgaacttagc tttgatttct gcgatcttcg ccagagcctg tgcacgattt agaggtctac
     2101 cgcccatgac aggaagttgt tttactggtt caggtatcgc ctcaccacgg ttaattcgcg
     2161 cggtcataca ggacagttca tcggcagcct tgcgccgtaa ttccgcgtca gtcaacgcat
     2221 tggcccgcat gttctgatac aggttggtaa ccagccagta gtgcgcgttt gatttccacg
     2281 gataagactc tgcatccgga tacagaccac gcttccggca atactcgtaa accatatcaa
     2341 ccagctcgct gacgtttggc agcccggcgg taacggatgc ttcttcccgg caccatgcaa
     2401 caaactgccc gggtgatggc agaaatggtc tattctgccg acgggcaaca cgcattccgg
     2461 cgctcacctg ttccatcgtg gtgatcccgt tttcccggaa agccagaacc cactggcggc
     2521 ggatttcgtt cagttcgttc tggtcacgat tagccaggct cgccgggaaa gttgccagta
     2581 actggctgaa tacaccgttg attatctgcg ctacctgctg tacctgcggc ttttcgtcgt
     2641 actgttccgg catgttgttg gcgatccgac gcatctgctc acggtcaaag ttaatcatct
     2701 gtgcggcgat gtttttcata gatccacccc gtaaatccag tctgtgtttg tcaggtcgag
     2761 ttttggtttg ctagctgtca cgcctgcctg ttgcttgtta cggttgattt cgagttgggt
     2821 ccacttgtcg cggagtttgg ccggacttag cacgttaccg gaccagaagt tgtcctggca
     2881 tgcccagcgg aacagcacgc acatgtcgcg gtggttacgt ccgtcacgtt cacgcatcag
     2941 gcggatatcg ttagcccacc ctgcaaaatt cggttttctg gctgatggcg cgatggtctt
     3001 caccatgtca aacatccact ctgcggcggt caggtcttct gctgtccccc acttgctgcc
     3061 gctctgaatt gcagcatccg gtttcaccac aggaagatcg ttttctggtt ggtcagagga
     3121 ttcgccagaa ttctcggacg aaaaaggttt tatattgtct tttgttagtt tgtcttttgt
     3181 gtttacctga ttcgggtaaa cgcctttacc tgatttgggt aaactttttt tacctgattc
     3241 aggtaaattt acctctttca ggtaaacttt atttttctta cctgattcgg gtaatgttga
     3301 ccattcactg accacattat taatgccggt attccgcccg ctctgaataa aaatcccacg
     3361 ctttaccaga acactttttg cagcagaaca cttgtgcggc aatatcccgg ttaattcgga
     3421 aagttgctcg ttgctaaccc aatccagttt tttattaaag ccatatgttt tgcgcatgac
     3481 agccagaaag accagaagct ggtgctgtgt taatccggcc agcatcacag cttccagcaa
     3541 ctcatttgca atgcgcgtat aaccatcatc gagatctgcc acgcgcggct ccttttgtgc
     3601 cgcatccggc actggaaaat tgaatatctc agcagtgttt gccataattc ctcccgcaat
     3661 gagtgtgtta cgatttgcac ctgaaagtcg gttctgttcc agcagaccgg ctttcgccat
     3721 ttctgaacct gtcatatcgc ccccagcatg gtagtaacca tcgccatcaa tggaccagcc
     3781 agatctgggt ccacacgaaa catcgacaca ataccttcac taatttcctt cagtttctgg
     3841 tggcgtggtg cgttgagaat gacagcctgt tttgcctcac tgagttcctt ttccatttca
     3901 gccaacctag ccatgaagct atcctgctca accaggtaac cgcgatattc cagcggtagt
     3961 accgccagaa ttgccggggt cagttcacgc acgttatttc ggtatttttc agaatcgaat
     4021 ttgttatcga ggaagcggaa cagcttctgg cgtgcacggc tgacatcatc agggaaatcg
     4081 atggtgccgc cgccctgctc ccgatactca ttcacaatga gtgtggcaac gacatcctga
     4141 ttatctacag ccgaccaggc gcggacggca tcacggattt tttcgtggcc tggcacctgt
     4201 tttgtttgag aacgatttat caccgcagtc gggctaaatc cgctagtctg ttggtatgta
     4261 agtggttgca taattgactc ctttagtttg aattgactgt taagttgatt gcttattgtt
     4321 aaagagcgtg aaatggaaat ttaagctgcg ttcttttcgg tgtgtggaaa caacttcgga
     4381 agatccgggc gaatctggta tgccttcact actccaccag tagccgtaac aatgctgccg
     4441 acatgttcag gggatacctt tgctttgttg tgaagccact tatagacggc ctgctgtgaa
     4501 acttcgcaag cagcgcccag tttcttttgt gaaccaacga tattgatcgc tgttttgata
     4561 gctgggttca taacaacctc cgtggttaat ttgaatcaag attaaaacta tggttgtttt
     4621 tagtcaacaa ccattttcgt ttgatggaat aaaaccttgg ttgtacattt ggactatgaa
     4681 aacaacactc tcagaaagac ttaaagaagc cagattagcg cgaggcctta cacaaaaggc
     4741 gcttggggat ttggtcgggg ttagccaggc tgctattcag aaaatcgaaa cagggaaagc
     4801 taatcaaaca actaaaatcg tggagatcgc gaacgctttg ggtgtgcgcg cagaatggtt
     4861 atcttctggc gttggaaata tgtcagacag tacagtgcaa ccaatacaat caactgtcag
     4921 ccattccaaa tacttcaaga ttgacgttct tgatatagaa gtcagtgctg ggccgggagt
     4981 catcaaccgt gagtttgtag aagttctacg ctcggttgag tactcgtttg acgatgctcg
     5041 tcacatgttc gatggtagga aggcggaaaa tatccgcatc attaacgtgc gtggtgacag
     5101 catgtcagga acgatcgaac caggtgatct gctgttcgtt gatatcacag ttaaatcttt
     5161 cgacggtgat ggtatctatg cgtttctgta cgacgacaca gcccatgtaa agcgcctgca
     5221 aatgatgaag gataagctgc tggtcatctc tgataacaaa agctactcac cgtgggaccc
     5281 gatcgagaaa gacgagatga accgggtgtt catcttcggt aaggttattg ggagcatgcc
     5341 gcagacatat aggaagcatg ggtagtacca attaaaaatt atcaactggg cattgtgctc
     5401 attcagtaaa gaactaattc ctatctttgc tctaggtagt aatattaagc caccgcaata
     5461 atatctttac ctaacggcgt aagaatcccg gtcaccgtgc cgggttttct tttgccctcc
     5521 cctcatcaca cacaccgtta aaaaaaccac cataacctcg cttcagttat cgctatgcga
     5581 ttcaagtcac aaaataaatc catcctaaat acaaccagtt atatctaaaa caaccaataa
     5641 aacaactttt gttgttgacg ataaaacaac tatagtttta aataagttca tcgcaacaac
     5701 acaacgatac ggcaactacc tgattcaccg ttgcgatgac cgcttagatc cgcagtttga
     5761 atttcagcag gcttcgggga gtgcgagggg tgaaacggac gcgtgaacgt cggtgtgacc
     5821 agctgaaatt aactcaacat ttcatacctt agtcgcttca acgaggcggc ttagttatga
     5881 caaccggcgg ccatccaccg cctgaatacg cgcagaagtc tctatatgtt cagcagccca
     5941 gcttacgggc aggagttttt atggttcatc aacattatgg aacgcagacc gttaatcgag
     6001 gtgcggtcat gccaggaatg ctggtcaaac acaaagatgg tacctggact gcatcagcta
     6061 atttacgcgg acggctttat ctgcatcgcg gcatcgagcg cacttatacc cgtgatttgc
     6121 tcgtggaagt ttttctcgac ggacgcggta acggcctgaa tcactaatcc cctttcctgt
     6181 tttcctaatc agcctggcat ttcgcgggcg atattttcac agccattttc aggaggtcag
     6241 ccatgaacgc ttattacatt caggatcgtc ttgaggctca gagctgggcg cgtcactacc
     6301 agcagatcgc ccgtgaagag aaagaggcag aactcgcaga cgacatggaa aaaggcctgc
     6361 cccagcacct gtttgaatcg ctatgcatcg atcatttgca acgccacggg gccagcaaaa
     6421 aagccattac ccgtgcgttt gatgacgatg ttgagtttca ggagcgcatg gcagaacaca
     6481 tccagtacat ggttgaaacc attgctcacc accaggttga tattgattca gaggtataaa
     6541 acggatgagt acagcactcg caacgctggc agggaagctg gctgaacgtg tcggcatgga
     6601 ttctgtcgac ccacaggaac tgatcaccac tcttcgccag acggcattta aaggtgatgc
     6661 cagcgatgcg cagttcatcg cattgctgat cgtcgccaac cagtacggcc ttaatccgtg
     6721 gacgaaagaa atttacgcct tccctgataa gcagaacggc attgttccgg tggtgggcgt
     6781 tgatggctgg tcccgcatca tcaatgaaaa ccagcagttt gatggcatgg actttgagca
     6841 ggacaatgaa tcctgtacat gccggattta ccgcaaggac cgtaatcatc cgatctgcgt
     6901 taccgaatgg atggatgaat gccgccgcga accattcaaa acccgcgaag gcagagaaat
     6961 cacggggccg tggcagtcgc atcccaaacg gatgttacgg cataaagcca tgattcagtg
     7021 tgcccgtctg gccttcggat ttgctggtat ctatgacaag gatgaagccg agcgcattgt
     7081 cgaaaatact gcatacactg cagaacgtca gccagaacgc gacatcactc cggttaacga
     7141 tgaaaccatg caggagatta acactctgct gatcgccctg gataaaacat gggatgacga
     7201 cttattgccg ctctgttccc agatatttcg ccgcgacatt cacgcatcgt cagaactgac
     7261 acaggccgaa gcagtgaaag ctcttggatt cctgaaacag aaagccactg agcagaaggt
     7321 ggcagcatga taccggacat tatcctgcag cgtaccggga tcgacgtgag agctgtcgaa
     7381 cagggggatg atgcatggca caaattacgg ctcggcgtca tcaccgcttc agaagttcac
     7441 aacgtgatag caaagccccg ctcaggaaag aagtggcctg acatgaaaat gtcctacttc
     7501 cacaccctgc tggctgaggt ttgcaccggt gtggctccgg aagttaatgc taaggcgctg
     7561 gcctggggaa aacagtacga gaacgacgcc agaaccctgt ttgaattcac ttccggcgtg
     7621 aatattactg aatccccgat catctatcgc gacgaaagta tgcgcaccgc ctgctccccc
     7681 gatggtttat gcagtgacgg caacggcctt gaactgaaat gcccgtttac ctcccgggat
     7741 ttcatgaaat tccggctcgg tggtttcgag gcaataaaat cggcttacat ggcccaggtg
     7801 cagtacagca tgtgggtgac gcgaaaagat gcctggtact ttgccaacta tgacccgcgc
     7861 atgaagcgtg aaggcctgca ttatgtcgtg attgagcgga atgaaaagta catggcgagt
     7921 tttgacgaga tggtgccgga gttcatcgaa aaaatggacg aggcactggc tgaaattggt
     7981 tttgtatttg gggagcaatg gcgatgacgc atcctcacga taatatccgg gtaggcgcga
     8041 tcactttcgt ctactccgtt acaaagcgag gctgggtatt tcccggcctt tctgttatca
     8101 gaaatccact gaaagcacag cggctggctg agaagataaa taataaacgg gaggcggtat
     8161 gcacaaagca tctcctgttg agttaagaac gagtattgag gtggcacata gccttgctca
     8221 aattggagtc aggtttgtgc caataccagt agaaacagac gaagaatttc atacgttagc
     8281 cacatccctt tcacaaaagc tggaaatgat ggtggcgaaa gcagaagcag atgagagaga
     8341 ccaggtatga caaccactga atgcattttt ctggcagcgg gcttcatatt ctgtgtgctt
     8401 atgcttgccg acatggggct tgttcaatga cacctcagca agaaaacgcc cttcgcagca
     8461 ttgcccgtca ggctaattct gaaatcaaaa aagccagaca gcattttccg gataaaaacg
     8521 tcgatgacat ttgccgtagc gtactaaaga agcaccgcga aacggtaacg ctgatgggat
     8581 tcacaccgac tcatttaagc ctggcgatcg gcatgttgaa cggcgtcttt aaggaacggt
     8641 aaacatgaaa agcaaaatca tcagggagct acaggctcct tttttattat tcgcatttac
     8701 cctcaagcgt attaaccaac aattcaggga ttaatgaaag atggcagaca tcattgattc
     8761 agcatcagaa atcgaagaat tacagcgcaa tacagcaata aaaatgcgtc gtctgaacta
     8821 ccagactgta tccgcaactc attgttgtga gtgtggcgat ccgatagatg agcgaagacg
     8881 cctggctgtt cagggttgtc ggacttgtgc gagttgccag gaggagatcg aacttaagaa
     8941 caaacaatag ggactgtgat ggcctcaaag cagcaaattt caacatcgtc caactgaggt
     9001 gtaaaaatgt tcagaatcat ttttcctaac acctggtacg tcgaccacca cggcactccc
     9061 tgcaaaatcc tgcgttctac ccacaacaaa gttcactaca tccgaaaagg cagaacatgt
     9121 atcgccagca tgttccgctt taatcatgac tttgaacctg tgaataaagc tgatgcagat
     9181 cggatagcag aagagatcga aacggcagaa cacattaaga agttacgtga catgcgttca
     9241 aaaagcagag gtaaccatgg aatcatacag cctcacactc gatgaggcct gtcagtttct
     9301 taagatatcc agaccaaccg ccaccaactg gatacgaaca ggccgcctac aggcaacacg
     9361 taaagatcca accaagccaa aatctcctta cctcacaaca cggcaagcct gcattgcggc
     9421 gcttcagtct ccgctgcata ctgtccaggt gagcgcgggt gatggcataa cagaggaaag
     9481 aaaatgtcac tcttccgcag aaatgaaata tggtatgcct cgtattcgct cccgggcgag
     9541 aaacgaatta aggaatctct tggcacaaag gacaagcggc aagctcagga gttgcacgac
     9601 aagcgaaaag cagaactctg gcgagtagaa aagctagggg atttacctga tgtcactttt
     9661 gaagaggcct gcctaagatg gcttgaggaa aaagctgata aaaaatctct cgattcagat
     9721 aaaagccgga ttgagttctg gcttgaacat tttgagggta taaggcttaa agatatctcg
     9781 gaggcaaaga tttactctgc tgtaagcaga atgcataaca gaaagacgaa agaaatatgg
     9841 aaacagaaag ttcaggccgc catcaggaaa ggtaaagaac cgcctgttta tgaaccaaag
     9901 ccagtatcaa ctcagacaaa ggcaaagcat cttgccatga taaaggccat tctccgtgct
     9961 gcagaacgcg actggaagtg gctggaaaaa gcgcctgtca tcaagatacc agcggtcaga
    10021 aacaagcgag tcagatggct ggaaaaggag gaagcaaaac gccttattga tgagtgcccc
    10081 gaaccactga aatctgtcgt caagtttgcg ctggcaactg gtctgagaaa gtcgaacatc
    10141 ataaatctgg aatggcaaca aatcgacatg cagcgacgag ttgcctgggt gaatccagaa
    10201 gagagcaaat caaaccgcgc cattggtgtg gcgctgaacg ataccgcctg taaagtgttg
    10261 cgtgatcaaa taggcaagca tcacaaatgg gtgtttgtac ataccaaggc ggctaagcga
    10321 gcagatggaa catcaacgcc tgcggtcagg aagatgcgca tcgacagcaa gacatcatgg
    10381 ctatcagctt gtcgtcgtgc aggaattgaa gatttccgtt tccatgacct cagacacacc
    10441 tgggcaagct ggctgattca gtcaggcgtc ccattatcag tgcttcagga aatgggcgga
    10501 tgggagtcca tagaaatggt tcgtaggtat gctcaccttg cgcctaatca tttgacagag
    10561 catgcgagga aaatagacga catttttggt gataatgtcc caaatatgtc ccactctgga
    10621 attatggagg atataaagaa ggcgtaactg attgaattgt aatggcgcgc cctgcaggat
    10681 tcgaacctgc ggcccacgac ttagaaggtc gttgctctat ccaactgagc taagggcgca
    10741 ttgataccac ggtgtgctgt aatcgcgtga attatacggt caacccttgc tgagtcaatg
    10801 gcttttgaac cagttgctga acaagtgtac gaccgcatca gatgtagctc actatggcgg
    10861 caaaccaacg acggttatca tgataggtgt gaatcagcca ggtacagaag gttttacttt
    10921 tcagaaaacg aaaactgaca gcgcgccacg cttctgacaa aatagacgca tccccttcga
    10981 cctacgtaac agatggaatc ctctctctga tggcagcaaa gattattgac ggtaaaacga
    11041 ttgcgcagca ggtgcgctct gaagttgctc aaaaagttca ggcgcgtatt gcagccggac
    11101 tgcgggcacc aggactggcc gttgtgctgg tgggtagtaa ccctgcatcg caaatttatg
    11161 tcgcaagcaa acgcaaggct tgtgaagaag tcgggttcgt ctcccgctct tatgacctcc
    11221 cggaaaccac cagcgaagcg gagctgctgg agcttatcga tgcgctgaat gccgacaaca
    11281 ccatcgatgg cattctggtt caactgccgt taccggcggg tattgataac gtcaaagtgc
    11341 tggaacgtat acacccggac aaagacgtgg acggtttcca tccttataac gtcggtcgcc
    11401 tgtgccagcg cgcgccgcgt ctgcgtccct gcaccccgcg cggtattgtc acgctgcttg
    11461 agcgttacaa cattgatacc ttcggcctca acgccgtggt gattggcgca tcgaatatcg
    11521 ttggccgccc gatgagcatg gaactgctac tggcaggttg caccaccacc gtgactcacc
    11581 gtttcactaa aaatctgcgt caccacgtag aaaatgccga tctactgatc gttgccgtcg
    11641 gcaagccggg ctttattcct ggtgactgga tcaaagaagg cgcaattgtg attgatgtcg
    11701 gcatcaaccg tctggaaaat ggcaaagttg tgggcgacgt cgtgtttgaa gacgcggcta
    11761 aacgcgcctc atacattacg cctgttccgg gcggcgttgg cccgatgacg gttgccacgc
    11821 tgattgaaaa cacgctacag gcgtgcgttg aatatcatga tccacagggt gagtaacatg
    11881 gcgacatttt ctttaggtaa acatccgcac gttgagctgt gcgacttgct gaaactggaa
    11941 ggctggagcg aaagcggcgc gcaggcgaaa atcgcgattg ccgaaggcca ggtgaaagtc
    12001 gacggtgcgg ttgaaacgcg caaacgctgc aaaatcgtcg ccgggcagac agtgagtttt
    12061 gcaggtcaca gcgtacaggt tgttgcctga tccctttccc ggcctgtaac aacaggccgg
    12121 ttctcttttc agacttttgc cctctacgcc ccgttcatcc atgctgatag catcgacatt
    12181 ttgccgaagc gaccatcatg ccaaccgtta ttacgcacgc cgccgttccc ctttgcattg
    12241 gtttagggtt gggttcgaaa gtcatccctc cgcgtctgtt atttgccgga atcgtcctgg
    12301 cgatgctgcc cgatgccgac gtgttgtcgt ttaaatttgg cgttgcttac ggcaatgttt
    12361 ttggtcatcg cgggtttacc cattcgctgg tgtttgcgtt tgttgtcccg ctgttatgtg
    12421 tgctgattgg gcgtcgatgg ttcagggctg ggctgattcg ctgctggctg tttttaaccg
    12481 tctcgttgct atcgcacagc ttgctggatt cggtaaccac tggcggtaaa ggggttggct
    12541 ggctgtggcc gtggtcagat gaacgctttt tcgctccctg gcaggtgatt aaagtcgcgc
    12601 cgtttgcatt gtctcgttac accacgccgt acgggcataa ggtgattatt tctgaattga
    12661 tgtgggtgtg gttgccgggg atgctactga tgggaatgtt gtggtggcgc agacgataac
    12721 cggatgcgaa aactcgcatc cggcaatagc gcaattactt acgacgccag gtggtccctt
    12781 gcgggccatc ttccagcacg atccccatct cgttaagacg gtcacgcgcc gcatctgccg
    12841 ccgcccagtc tttcgcttta cgggcatcca gacgctgttg aattaacgct tcaatctcag
    12901 ccacttcact gtcgtctgcc tgcgcgccgc tttgcaggaa cgcttccggt tcttgctcca
    12961 gcaggcccaa tacagcggaa agtttacgca ggtgagacgc cattgcattc gccgctgcca
    13021 tatcttctgc tttcagacgg ttcacttcac gcgccatatc aaacagtacg gaataggctt
    13081 ccggggtgtt gaaatcgtcg ttcatcgcct caataaagcg cgcttcaaac gcttcgccac
    13141 cggcaggagc aacggttttg tctgtgccgc gcaaggcagt atagagacgt tccagcgccg
    13201 cacgcgcctg cttcaggttc tcttcgctgt agttcaactg actgcgatag tgacccgaca
    13261 tcaggaagta acgcacagtt tccgcatcgt agtatttcag cacatcacgc acggtgaaga
    13321 agttacccag cgatttagac atcttctcgc ggtcaaccat caccatcccc gagtgcatcc
    13381 agtagttcac atactgacca tcatgggcac aggtggactg ggcaatttcg ttctcgtggt
    13441 gcgggaacat caggtcagaa ccgccgccgt ggatatcaaa gtggttaccc aactgtttgc
    13501 agttcattgc cgaacactca atgtgccagc caggacggcc cgcgccccac ggagacggcc
    13561 agctcggttc gccctctttc gacatcttcc acagaacgaa atccatcggg ttgcgtttgt
    13621 cgtcgaccac gtcaacgcgc gcgcctgcct gcagctgatc gagatcctga cgcgacagca
    13681 cgccataagt tggatcggtc gggacgtcga acatcacgtc gccgttgtcc gccacataag
    13741 cgtgaccttt ggcgatcagt tgttcagtga gttcaataat ttcggcgata tggtgcgtcg
    13801 cgcgcggctc catatccggg cgcagaatgt tcaaagcatc aaaatctttg tgcatttcgg
    13861 cgatcatgcg atccaccagc gccacaaagc tttcgccatt ttcattggcg cgtttgatga
    13921 ttttgtcgtc gatatcagta atgttgcgca catacttcag cttatagccg aggaaacgca
    13981 gatagcgcgc aaccacgtca aaggcaacaa aggtacgccc gtgaccgata tgacagagat
    14041 cgtaaacggt gattccacac acgtacatgc cgacttcccc ggcgtgaata ggcttaaatt
    14101 cctctttttg gcgtgtcaga gtattgaaga tttttagcat cgaagattcc gtttagacat
    14161 gtgtgggtaa ttgagttgcg tataataccc atatttcccg cgcgaatcag catacattgc
    14221 gtgatgatcc gatcgtgcgg ttatgctata acaccaccct atatatgacc cgaaccgggt
    14281 tgaagcacca atcaaacgga acaggatgca aaaatggtta ctttccacac caatcacggc
    14341 gatattgtca tcaaaacttt tgacgataaa gcacctgaaa cagttaaaaa cttcctggac
    14401 tactgccgcg aaggttttta caacaacacc attttccacc gtgtaatcaa cggctttatg
    14461 attcagggcg gcggttttga accgggcatg aagcaaaaaa ccaccaaaga cccgatcaaa
    14521 aacgaagcta acaacggtct gaaaaatacc cgtggtacac tggcaatggc ccgtactcag
    14581 gcaccgcact ctgcaactgc acagttcttc atcaacgtgg ttgataacga cttcctgaac
    14641 ttctctggcg aaagcctgca aggttggggt tactgcgtgt ttgctgaagt ggttgaaggc
    14701 atggacgtgg tagacaaaat caaaggtgtt gcaaccggtc gtagcggtat gcaccaggac
    14761 gtgccaaaag aagacgttat cattgaaagc gtgaccgtta gcgagtaatc gtggcgacac
    14821 tctttattgc agatcttcat ctctgcgcgg aagaaccggc gatcaccgcc ggttttctgc
    14881 gttttttagc gggggaagcc cgcaaggccg acgcgctgta tattcttggc gatctgtttg
    14941 aagcatggat tggcgacgac gatcccaacc cactccatcg ccagatggcg gcagcgatca
    15001 aagcggtgtc cgattccggc gttccctgtt atttcattca tggcaaccgt gattttctgc
    15061 tcggcaaacg ctttgcccgt gaaagcggca tgacgttatt gccggaagaa aaggtgctcg
    15121 aactttatgg tcgccgggtg ttgattatgc atggcgacac gctgtgcacc gatgacgccg
    15181 gttatcaggc ttttcgcgcc aaagtccaca aaccctggct gcaaacgcta ttcctcgccc
    15241 tgccgttgtt tgtgcgcaaa cgcattgccg cgcgaatgcg cgcgaacagc aaagaagcca
    15301 acagcagtaa atcgctggcg atcatggacg ttaaccaaaa cgcggtggtc agtgcgatgg
    15361 aaaaacatca ggtgcaatgg ctgattcacg ggcataccca tcgcccggca gtacatgaac
    15421 ttatcgccaa tcagcaacct gctttccgcg tcgtgctggg tgcctggcat actgaaggtt
    15481 cgatggtgaa agtcacggcg gatgacgttg agttgatcca ttttccattt taaaaaacgc
    15541 gcaactttgc tgatttcata accgcgcaac cgttttcctt gctctctttc cgtgctattc
    15601 tctgtgccct ctaaagccga gagttgtgca ccacaggagt tttaagacgc atgtcttccc
    15661 gcaataatcc ggcgcgtgtc gccatcgtga tggggtccaa aagcgactgg gctaccatgc
    15721 agttcgccgc cgaaatcttc gaaatcctga atgtcccaca ccacgttgaa gtggtttctg
    15781 ctcaccgcac ccccgataaa ctgttcagct tcgccgaaag tgccgaagag aacggttatc
    15841 aggtgattat tgcgggcgca ggcggcgcag cgcacctgcc aggcatgatt gccgccaaaa
    15901 cgctggtgcc agtgctgggc gtgccagtac agagcgccgc actgagcggt gtcgatagcc
    15961 tctactccat cgtacaaatg ccgcgcggca ttccggtggg tacgctggcg attggtaaag
    16021 ctggcgcggc aaacgcggcg ttactggcag cacaaattct tgcgactcat gataaagaac
    16081 tgcaccagcg tctgaatgac tggcgcaaag cccagaccga cgaagtgctg gaaaacccgg
    16141 acccgcgagg tgcggcatga aacaggtttg cgtcctcggt aacgggcagt taggccgtat
    16201 gctgcgtcag gcaggcgaac cgttaggcat tgctgtctgg ccggtcgggc tggacgctga
    16261 accggcggcg gtgcctgttc aacaaagtgt gattaccgct gagatcgaac gctggccgga
    16321 aaccgcatta acccgcgagc tggcgcgcca tccggccttt gtgaaccgcg atgtgttccc
    16381 gattattgcc gaccgtctga ctcagaagca gcttttcgat aagctccacc tgccgaccgc
    16441 gccgtggcag ttacttgccg atcgcagcga gtggcctgcg gtgtttgatc gtttaggtga
    16501 actggcgatt gttaagcgtc gcactggtgg ctatgacggt cgcggtcaat ggcgtttacg
    16561 cgcaaatgaa accgaacagt taccggcaga gtgttacggc gaatgtattg tcgagcaggg
    16621 cattaacttc tctggtgaag tgtcgctggt tggcgcgcgc ggttttgatg gcagcaccgt
    16681 gttttatccg ctgacgcata acctgcatca ggacggtatt ttgcgcacca gcgtcgcttt
    16741 cccgcaggcc aatgcgcagc agcaagcgca agccgaagag atgctgtcgg cgattatgca
    16801 ggagctgggc tatgtaggcg tgatggcaat ggagtgtttt gtcaccccgc aaggtctgct
    16861 gatcaacgaa ctggctccgc gtgtgcataa cagcggtcac tggacacaaa acggtgccag
    16921 catcagccag tttgagctgc atctgcgggc gattaccgat ctgccgttac cgcaaccggt
    16981 agtgaatagt ccgtcggtga tgatcaacct gattggtagc gatgtgaatt atgactggct
    17041 gaaactgccg ctggtgcatc tgcactggta cgacaaagaa gtccgtccgg ggcgtaaagt
    17101 ggggcatctg aatttgaccg acagcgacac atcgcgtctg accgcgacgc tggaagcctt
    17161 aatcccgctg ctgccgccag agtatgccag cggcgtgatg tgggcgcaga gcaagttcag
    17221 ttaattgatg ctcattttct tgccggatgc ggcgtgaacg ccttatccgg cccaccgctc
    17281 caacaccgac tgtaggcctg ataagacgcg ccagcatcgc atcaggcatg gtgtgccgat
    17341 tgcaggatgc ggcgtgaacg ccttatccgg cctaccgttc caacaccgac tgtaggcctg
    17401 ataagatgcg ccagcatcgc atcaggcatg gtgtgccgat tgccggatgc ggcgtgaacg
    17461 ccttatccgg cctaccgttc cgacaccgcc tgtagacctg ataagacgcg ccagcatcgc
    17521 atcaggctag atgtgccaat tgcctggtgc ccatcactac agcgaaatac aagtccccgc
    17581 ttcgcccgcc agcgtctctt caatccgcga taacgcccca atccacgcgg gtttgccacg
    17641 gcttctgaca tagccactca ccgcagtcac cttcggcccc atcgaaccat cggctttggc
    17701 aaatggcgct aactcatccg gtgtggcatg gcgaatggca cgttgctgcg gcgttcccca
    17761 gttttcatat accgcatcag catcggtgag gatcaccagt ccatcggcat taatctgctc
    17821 ggcgagcaac gcagcggcga gatctttatc aattaccgct tcactccctg ccccgtcttt
    17881 cgtcacaggc acaccgccgc cgccactgca aatcaccaca tgcccctctt tgagcaacaa
    17941 ctcgatggct tcgctgtcga gaattttacg cggttgcgga gacgccacca cccggcgtaa
    18001 atatttacca tcacgtttca tctgccagcc ataagccgct tccagtgcct cttgttcttc
    18061 tggctgataa accggaccga taaacttctc tggctgcaaa aacgccggat catcaggcga
    18121 aacttcaatg cgcgtcagca ctgtcgtgac aggcggcatc tgcggctgtg cgctcaagct
    18181 ctgcgccagc atatagccaa tcatcccctg gctttccgcc accagcacat ccagcggata
    18241 cggttctacc tctttccacg ccagattctg taacgccagc agccccacct gcggcccgtt
    18301 gccgtgaaca atcgccagcc gataagaacg ggccaggcgc gccagtgcgg gtacagcact
    18361 ggcaatgttg cgatattgat tttctgccgt cagcgcctca ccgcgctgga gtaaggcgtt
    18421 gcccccaaga gccacaacca gtgttttcat gattttcctt gtaataattg ttggccaagc
    18481 cagaacccca gcagcgtatc tgcccctgac gtatgcccca gcgccagcag cgaatcaatc
    18541 gcaacagcgg tacgtttcgg gcaactcaga gcatgaataa agtgcaggag tggcgaggcg
    18601 aagtatcctt gcgcggcata acgtaaataa ctgacgctca ccgctgtggt aacgagttga
    18661 agattgtcgg aacaggcaaa aaacgggcga ccggagcgcg catctaaagc gccataatac
    18721 caggccgcca gcagcattcc gctcagcgtg tcatcatgac ttggcgttaa tcctgggcct
    18781 ttacccagcc agtgccgcca gtcggtctta acgccattga gcgcggcctg aaaacagtga
    18841 cgaaactggc gtaattcagc aggaagcgga tcgcctgccg ccaacgccag tggcccgaaa
    18901 agcccggttt cctccgcgcg ttgcatccat gccgctgcaa gcggttgagg atgcgcaggc
    18961 ggcgtaatac gcagcaaaca ataacgctgt ggctgtttaa ccgtgaaacg tccgaggcga
    19021 atcccttgag ccataacctg tggtcgttca ttgccgcata atccaccgca taacgcatcg
    19081 aattgcgcgc ggcgaagcac ccatcctccg gggccgaaac cactaccctg acgatgcagc
    19141 gttaacagtt cgccgctttc cgtcatcagg ttaatcgccc gccgccacac tcccgctaac
    19201 cgccaggact ggcgataatt cggtgcgcta ctactggcaa gcagaggatg gatgatcgtc
    19261 attgtgcgtt cctcaaccaa tccccatgct ttcagccagt gcttccagcg cctgctcaaa
    19321 gcacgccagc ggtgcccgca cggtgcctgc gccaatctgc ccgatccccg cctctttatg
    19381 ggcgattccg gtattgatga gcggcgtaat gccggtttct accacgcgac gaatatccag
    19441 ccccaggcac gcgccctgga aatcccagcc ggggatctgc aactgcatat tgcgttcaag
    19501 gaagatttcc gccatctctt cagataccgc tctggccgct tccatgccac ccgcaccgac
    19561 aaagcgcgtt acgccaggcg ctgcgatcat cgccgcaccg ccaataccaa aggtttcggt
    19621 aattgcgcta tcgcccatat ccgggttcgc ctgctcctgc gagaagccgg tgaaaaacag
    19681 cccttgggga gtgtttacag gcgcagtaaa ccagcgttcg cccagcccgc ttacccgaat
    19741 cccgaacata ttgccgttgc gggtcatagc cgtgacgatg ctgcctgcgc ggatcatcgc
    19801 accagcatcc atcgccgcct tgcagtaagc catcgcgagg ttaaggaaga actgatcggt
    19861 cacgctgagg aaatccatca cttccgcgat gtgctgttta tcatgatcga ggcgagcaat
    19921 gtgtggggcc agcgcacgca ttaacagtgc tgaggaagca atattgcgtt gatggaactc
    19981 atcgcccatg gtaatgccct gcgccatcat cgcggtgaga tcgataccgc gctccatgcg
    20041 ccccagcgcc gcgcttaata ctggcattaa cacatcgcgc atccagcgat ggcgagtcag
    20101 aacatcttcg ccgtaagcgc caaaacgcat cactttgccg ataccttcgt tgaggttgca
    20161 gtacgcccgg ttgccgtcgg tcacgttctc gaccaccagc atcggcatac tggcagaagt
    20221 aatgccgccc attggcccga cggcattcac atggtgacaa ggaatgaagt tcacttcccc
    20281 ctgatccagc attgccagcg cctgcgcttc atctttcgcc cagccttcaa acagacatgc
    20341 gcccacacat gcccctttca tgggtccggt catctcctgc cagcgcattg gcggcccggc
    20401 gtgaagcagt gttttgccct cgtttagttc gctgataagt gaagaagccg gttgcacatc
    20461 cagccagtgt ggacgagcgc gacgaatttg ttcgataacc gcagcattgg cttgcgccac
    20521 tgatgtaaac atgggaaccc cttattgcaa acgttctaat aaacgaacca gttttttatt
    20581 gccaccggcg actggcgacc attggtaatg cacaaccggt ttgctggcgc tttgtagctc
    20641 cagcgcaaag ctgcgtaatc cgatgttaat cacggcgacg ttttccagta atgacggtgt
    20701 atgtggctgt gtggcaggcg agagcggatg aattaacgcc gccgccagca aggtggcttc
    20761 cggcagcgaa ctcacgaccg caatccccgc atcttccagc gtggcgattt gctgcgagcg
    20821 gcattgcggg tcacgttcag tgcctgtcac cgtggcaatg gcgtacagcg gttggctgtc
    20881 agaacgcgcg gcacaggctt tttgccaggc gctcaccagc gaggcggcag gatcggcggt
    20941 cgcaccgaag ccaatcacga catcaagcag caacacgcgc acttgcggtt tagcgccgag
    21001 atcggcaatt aacaggttgc gtaaggtcgg atcgatcatc ggatggggac gcccgacggt
    21061 gtaaaaatca tcgcccaggt cgaggatctg atgtccgtcg gcgtccagca tcatgccatg
    21121 ttgatgggta tcgtcggctt ccacgccaag gtgtccggca agtaatcccg ccgcttcagc
    21181 agccagcgta ccgccggtat acaagccgca aataaatccg ctgctgacag gcgctattgc
    21241 gttacgtcgc gcagtgacgc gtgaaagcag acaagccagg cgtgcggcct catccagcga
    21301 ggaggcaaac cagacattct cgtcgcgggc caccgccggg gtataaccta aaaacagcgc
    21361 caccgtcggt ttgccagttg ctttcatggc attaacaatt ttcagacgca ccgcttcggc
    21421 aggtggtttt gaaacaaatg ccagcacttc gcttttctcg tctgcactga gcatttccag
    21481 cgccgtcagc gcactgatgc cgcccacttc acggctgagg tcgcgcccgc caaggccaat
    21541 cgcgtgagta attccctccc ctgccagcgc aatctgtgag cacagctcct gaatcccggt
    21601 accggaagcg ccaatgacgc caatgttgcc ttccggtatc acgttagcaa aagccagcgg
    21661 cgtgccggca atcatcgacg taccgcagtc cggccccatc accagcaagc ctttttcccg
    21721 cgcgcgggtt ttaagttgga tttcatcttc cagcgtgacg ttatcggaga acatcatcac
    21781 gttgaggttg cgatccagcg cctggttcgc cagctccgcc gcatactcac cagccactga
    21841 aatcagcgcc agattggcat cgggtaattt ctgacaggca ctgtcccagc gacgcacctg
    21901 cgtcaacgcc tggctgctgg ctgacccctg cgccagttgt tttagcgcct cttcaagctg
    21961 ctgcataatc gcctgcgcga tccccgcatc cgccgcttcg ctacgaattg ccacgcaaat
    22021 atcgttcggc gtggcgttat taaaatcgtc atgccagaaa cctgtggtat ctaataacgc
    22081 tttattggcg ggcgtaccca tcattacgga aacatcatca acattttctg attcgctgag
    22141 ttttcgtgaa ataatcatta aactgaccga atcctgaaaa cacccttttt taataaaggc
    22201 gtggatcata acaactcctt aaaaattgca cccatggcaa ttgcacgagc agtgttaatg
    22261 tggcgattac ggtatcgatg acaacgagta aataaggtga tgaatatcac acagggatta
    22321 ttaacaaaag aattcaaata accgccatgc tattacgtgt tattttttaa taagcgtggt
    22381 tgttttaaaa accaggttaa aagcgagcgg attatcaaat aaattctaac tatatttttt
    22441 tgcctgtctg gatcacataa tccagatatt ttccctgtat gttaattgca gtcatgcttc
    22501 acaccgtcgt taaaaaggaa gacagatgaa aatcagtcgg gaaacactcc accagctaat
    22561 tgagaataaa ctctgccagg ctgggttaaa acgtgagcac gctgcaaccg tggctgaagt
    22621 attggtttac gccgatgcca gagggatcca ctctcatggc gcggtgcgcg tggaatacta
    22681 cgcggaacgc atttcaaaag gcggcaccaa ccgcgaaccg gagtttcgtc ttgaggaaac
    22741 cgggccgtgc tcggcaattt tacatgccga caatgccgcc ggacaggtcg cggcgaaaat
    22801 gggtatggaa catgccatca aaaccgccca gcaaaatggc gttgcggtgg tcggtatcag
    22861 ccggatgggt cacagcggcg caatctctta ttttgtgcag caggcagctc gcgccgggtt
    22921 aattggcatt tcgatgtgcc agtccgatcc aatggtggtg ccgtttggcg gcgcggaaat
    22981 ttactacggt actaacccac tggcctttgc cgcgccggga gaaggcgacg agatccttac
    23041 ctttgatatg gcgactaccg tacaggcatg gggaaaagtg ctcgacgccc gctcgcgtaa
    23101 tatgtctatc ccggatacct gggcggtcga taaaaacgga gcaccaacaa ccgatccatt
    23161 tgcagttaac gccctgctcc ctgccgccgg accgaaaggg tatggcctga tgatgatgat
    23221 tgacgtcctc tcaggcgtct tactcggctt accgttcggg cgacaggtta gttcgatgta
    23281 tgacgattta cacgccgggc gtaatttggg gcaattacat atcgttatta atccgaactt
    23341 tttctcctcc agcgaattat tccgtcaaca tcttagccag accatgcgcg aattaaatgc
    23401 cattaccccc gcgcccggtt ttaatcaggt ttattatccc ggacaggatc aggatattaa
    23461 acaacgcaaa gccgccgtcg aaggcatcga aattgttgat gatatttacc agtatttaat
    23521 ttccgacgcg ctttataaca cgtcatacga aacgaaaaat ccctttgcgc aataattatt
    23581 gagacaggac tttcttatga ttacacattt ccgtcaagcc atagaagaaa cgctgccctg
    23641 gctttcctct tttggcgctg acccagcggg tgggatgacc cgtttacttt attcgccgga
    23701 atggctggaa actcagcagc agtttaaaaa aagaatggca gcaagcgggc tggaaacacg
    23761 tttcgatgaa gtggggaatt tatacggtcg cctgagtggc accgaatatc cacaggaagt
    23821 ggttctgagc ggttcgcata tcgataccgt ggttaacggc ggtaacctcg acgggcaatt
    23881 cggtgcgctg gcggcgtggc tggcaattga ctggctgaaa acgcaatacg gcgcgccgtt
    23941 acgtacggtc gaagtggtgg cgatggcaga agaagaaggc agccgcttcc cgtatgtctt
    24001 ctggggcagt aaaaatatct ttgggctggc gaatcctgac gacgtgcgga atatctgtga
    24061 tgccaaagga aatagttttg tcgatgcgat gaaggcttgc ggatttactc tgccgaacgc
    24121 cccactaact ccgcgtcagg atattaaagc ctttgtcgaa ctgcatatcg aacagggctg
    24181 tgtgctggaa agtaatgggc agtcaattgg cgtggtgaat gcaattgtcg ggcagcgtcg
    24241 ttatacggtg acgctgaacg gcgaatcaaa ccatgcaggc accacgccga tgggttatcg
    24301 tcgtgatacg gtttacgctt tcagtcgcat ttgccatcag tcgatcgaaa aagcgaaaaa
    24361 gatgggcgac ccgctggtgc ttacctttgg caaggtggag ccgcgtccga atacggtgaa
    24421 tgtggtaccg ggtaaaacca cgttcaccat tgattgtcgt cataccgacg ctgccgtgct
    24481 gcgcgatttc acccagcagt tagaaaacga catgcgggcg atttgcgatg aaatggacat
    24541 tggtattgat atcgatttat ggatggacga agaacccgtg ccgatgaata aggagctggt
    24601 cgccaccctg acagaattgt gtgaaagtga aaaacagaac taccgggtaa tgcacagtgg
    24661 tgccgggcac gacgcgcaaa ttttcgcgcc tcgcgtgccg acctgcatga ttttcattcc
    24721 cagcatcaac gggatcagcc ataacccggc ggaacgcacc aatattaccg accttgccga
    24781 aggggtcaaa acgttggcac tcatgcttta tcaacttgcc tggcagaaat aaggagttat
    24841 aaatgggata tttaaataac gtcaccggtt accgcgatga tttactggct aaccgtgcga
    24901 ttgtgaaaca cggtaatttc gcactgttaa caccagacgg tctggtaaaa aatattattc
    24961 cgggctttga aaattgtgac gcgacaatcc tctccacgcc aaagctgggt gcctcttttg
    25021 ttgattatct ggtcacactg catcaaaacg gcggcaacca acagggcttc ggtggcgaag
    25081 gcattgaaac gttcctgtat gtgatctctg gaaatatcac tgccaaagcc gaagggaaaa
    25141 catttgcctt aagcgaaggt ggctatcttt attgcccgcc aggctcctta atgacgtttg
    25201 ttaacgccca ggccgaagac agccaaatct ttttatataa gcgtcgctat attccggtag
    25261 aaggtcatgc gccgtggctg gtttctggca atgccagcga actggaacac attcattatg
    25321 aaggcatgga cgatgttatt ctgctggatt ttctgcccaa agagttaggt tttgatatga
    25381 acatgcatat cctctctttt gcgccaggtg ccagccacgg ttatatcgaa actcacgtcc
    25441 aggaacacgg tgcctatatt ctttccggtc agggggttta taacctcgac aataactgga
    25501 tcccggtgaa aaaaggcgat tacatcttta tgggcgctta ctccttacag gccggttatg
    25561 gcgtagggcg tggtgaagcg tttagttata tttactcaaa agattgcaac cgcgacgtag
    25621 agatttaagt cataccactc gcctgattat ttttagctat gttggatttt gccacggttt
    25681 attccgtggc tttttttgta gtcaattcag gtaaaaaaag atgcgatata aatcgcatcc
    25741 gctttaaagg taagagtcag tttttaattc cctgacctat tttaatggcg caggcaatat
    25801 ttcgcgcgct gttaaagaga ttggtttcac cgctggcgag cacttcggct agaggtgcca
    25861 gacgtggcaa aatgctgaat accgcgtcaa tgccgtactg gtgcaccact tccacgccat
    25921 cacccaatac gccagcaatc ccaatcaccg ggacattaaa ctgcttcgcc accgacgcca
    25981 cgcccagcgg cgctttacca cctgccgttt gcgagtcgat gcgcccttcc ccggtgatca
    26041 ccagtgctgc gccctgcact gcctgcgcaa gattgaccgc attcaacaca atctcaatac
    26101 ccggtttaat atccgcattg aggaataccg ccgccgcaat gcccataccg cccgccgcgc
    26161 caccacccgc catctggcag acgttgattt cagtttgctg ttgtaaaaca cgggcgtaat
    26221 tttgcagccc ctgctccagc tcttcgacca tttccggcgt tgcccctttt tgcgggccga
    26281 acaccgccgc cgcgccgcgt gcccctacca gcggattatc aacatcacat gcgacttcaa
    26341 tatggcagtt cgccagacgc ggatcgcatt catccatctc aatacttgcc acgcgtgcta
    26401 aattgccgcc gttagccgcc agcacctgac cgtaggcatc aaggaaacgc acaccgagcg
    26461 cctgcgccat gcccataccg ccgtcaaccg tcgcgctacc accaatgccg agaataatat
    26521 ggcgaatgcc gttatccagc gcatggcgaa ttaactcccc cgtaccaaaa ctggaggcca
    26581 gcaacggatt acgcttttca ggcgcgacca gcatcaggcc acttgctgct gccatctcaa
    26641 tcaccgccgt tttcccgtcg ccggtaatgc cataaaaagc attcactttt tcgcccatcg
    26701 gcccgcagac ttcaagcttc acgatgttac cgtccgtcgc ggcgaccatc gcctccaccg
    26761 tcccttcgcc gccatccgct atcggcaaac agatatagtg cgcatcggga aagagggtcg
    26821 aaaacccggc tttaattgcc tgacaacatt tttctgcact taagctctct ttaaaagagt
    26881 ctggcgcaat gacaatcttc attatcaatc ctttttcaac aaattattcg atgcgttccc
    26941 acggaataag attatccata agaacagcca gtaaaattcc gaccagtaag ccgttactga
    27001 gcagaggacg aagcgttaat ggaaggtctt gcagatacac aggcggtaat gccattaaaa
    27061 atatgccgac aaataacggc aatgcgagtc gataaatatt gcgagcggta aacgtgattt
    27121 gctggctaaa caccagcgcg gaaaagagta aaggcagata agaaaccagc atgaccgcgc
    27181 tactcactgg taaagggata ctgcaaaaca gtcgcgtgag tgccggaacc agtgccacca
    27241 gcaggcaaat aacgctgcca taaataaacg aacgccgcgt gtaatcgcta gtctgggtta
    27301 ataaaccaat ggatgaaaca aacggtgaaa atggaattac tgccagtggc acggttatca
    27361 gtgtcataaa tccggtcgcc acaaagctgc gacgataacg cgtattcccc gcgccctgct
    27421 gcggataaaa aacatccgtg ccccgaatcg caccgtaggt attgctgata tttaccagac
    27481 ctgtaatcac cgccgtcaga ataatccccg gcgacaaagc accaccactg ccgagcggga
    27541 accactgcca gtgcaactca ccggagagcg aatgcgaaga aggaaagcaa aagtaccaca
    27601 acaaccagcc ggttatcgta ccgaccagca ggccgtaacg ggcaaaacgt tgtggcagga
    27661 aaataatcat cgccagtacc aggcacatca ccgcgaccga gagcgcgaac ggcggtaatt
    27721 gaattttaaa attcgggtcg gctatgccaa acggcagccc gagcatacct ttgaaaaaga
    27781 tagtggtcag ttgcgcaccc agcatcaaca taaacaagac catcaccgac ggcgtaaata
    27841 accgtgccag gcgatgtcct aatccgctaa aaccaatcaa catcgtcagc acgccggaga
    27901 gcgcaattcc caccgccaga ctggtagcga tatcgttgat cggtgtcccg cgcgatgctt
    27961 cgccaagggt gatagtcagg atggttcccc accacaggcc gcccggcccc tccataatag
    28021 cgcgacgatg accgcagaac gcctgagcga agcaggccag tgcggtagca agaaaagcat
    28081 attgcgtgag cgtaagcagg ctactttgcg gcaactgaaa agcagaaagt agcgtaggag
    28141 gaaccacaac cgtgttgcaa aaaataaaga aaaaccactg aaatcctgat aacaggcttt
    28201 cgcggctgac tgcaaaattg aacataaaaa caccttaacc ggagaatgcc cgccgcaagg
    28261 acgggcattg caggggccag attactgctg atgtttaagg ataaattgac ctttcggcgc
    28321 aacagggaag ccctgttcga tgtcgtaaat cacatcacca cgtaagatgg ttttcgtgat
    28381 acgcgcgcca atggtacggc caacatacgg gctgactttg tggcgatatt ccagatcgtc
    28441 attggtaaga acatagctgc tattcggctg aatgaagacg aagtcggcat cttttcctgg
    28501 ggcgatacgg cctttttgct gcagaccgaa aatatctgct gcgttagtcg ccattaattt
    28561 gccgaacatc ggcagagaca taccgcgttt ctgtaccgct tcatcgaaca tcacgtccat
    28621 gcagctttgc agaccggcga taccacccca tgctttcatg atattgccgg ctttcatttc
    28681 cggcggacat ggagagtggt cggaaaccag gcagtcgatt tcaccgttaa acagtttttc
    28741 ccacatgcct ttctggtttt ccagatcgcg gatcggcggt gaacacttcg ccagagtacc
    28801 gatttcttcg aactgatcgg tatcaagtac aaagtaatgc gggcaggatt cacaagtaac
    28861 gtcctgacct tcctgacgtg cacgagtcac ttcttcaacg ccttccgggc tgctgatatg
    28921 gcaaacgtgc agacggcaac cggcaacttt cgccagatac agcacgcggc gaatcgcttc
    28981 cacttcggta aataccggac gcgaagccac atagtcatga gcagttacgc gaccttcacg
    29041 cttcgcttct tcgcccagtt cgtcacagat cagcgcgttt tcgcagtgca ccagcaccgg
    29101 ctgccccagt tcgcccagct tctgcgcacc tttgaagaac tgccagtcgt ttacatcacg
    29161 gaagtcgttg tcgataccgc gatcgccaca ggtcgcaacg aagcatttga agccgacaac
    29221 gcccacttca tccagctcat gcagacggtc gatgttgtaa gacaccaggc caccgagttg
    29281 tgccgcatcg atagtcagct tgcctttagc ggcatcgaac ttcagttcaa ttgaagcgcg
    29341 gtcaaccgtt gctggcagct ggttgagcgg catttcgatc atggtggtga taccaccttt
    29401 tgccgctgcg cgagtaccag tttcataacc ttcccagtgg ctacgacccg gctcagaaat
    29461 atgggtgtgc gcatcaacca tgcctggaga aaccaccaga ccagacgcat ccatcacttc
    29521 ttttgcatcg cccagatcct gaccgatagc agcaattttt ccgcctttaa cggcgatatc
    29581 tacaacgcga gcttcgtttt ctaaaataac ggtgccgttt ttaatgatta aatcaaaaga
    29641 cataacaaac tccttgtaaa atacagattg gttttttaac tcaatgttca atatcgggat
    29701 taattaacct gtgacttttt gttctcctgt tttttctgct gctgtacgtt tttttaataa
    29761 cgcgtaggca gcgaatgcga caataacgcc gacaaaccat gaaacgcgtg ataaaggctc
    29821 catgaacgga ataaatttac caccgagaga taaaataacg gcgaccaaag ttactgagaa
    29881 cgcggtaagg ttaaagccgt tatcgtaata tttgaaatca cctgctgcgg tatacagttc
    29941 atcaagatta atttgtccgc gcatcaccat aaaataatgc gccatcatga caccaattac
    30001 cggacccagc attccgccga taatatcgag gaacagataa atactgtcct gattttccat
    30061 taatttccac gggcagatca ccaggctgat aatactggca atcagtacgc cgtttttata
    30121 ggtcagtttc gtcggtgcaa tagcggcaat ctgataaccg gccggaataa tattaccggt
    30181 ggcgttagtg gaaatggtcg tcatcagaat aaccagtacc gcaaagaacg aggcgaacag
    30241 gctgtcccaa cgctgaacaa tatccagcac gttccaggtg tcagcgccgt aatgaatact
    30301 ggctccggca ataatacata ccccggcgac cgcaaacaga atataggcca caactaaacc
    30361 cagcgtttgc cccagcgcct gctcacgaaa cgagtgggcg ttttgcgtaa agtcggatgc
    30421 gctcaccgcc ggtgccgccc agaccgcaac caccgcgttt atcaccacca gaaacaggaa
    30481 gccactgttt tctgctttct gaataccgcc cggaatgtag tcaaagattg gaccgatccc
    30541 gaccagcgaa atcgcccaaa tcgccatacc gccgaaaacg atatagatgc acgggttaag
    30601 aatggcagtg aatttattta aaacttttcc gccgccaaaa ccaataccaa cgttaaccag
    30661 ccagaagagt aaaaaagtaa ttaatcccgg tagagaaagg cctaacagcg tgaaatcgcc
    30721 accgagagtc aaaaatcccg gccagatttt gccaatcaga atcaagcagg ccagtgaccc
    30781 cgcgtaacat tgcaggccaa accacataat ggcagcaata ccgcctctta ataatccggg
    30841 aaacagtgca ccacgtacgc cgtaagaagc acgcaggatc atggcaaacg gtacgccgta
    30901 tttactgccc gcagcaccgt ttaataccat taccgcggca atgaaaaagg cactgaggat
    30961 aattgccagc ataatactaa aggtagacaa gccgagaata aaaaagccgc caaccatcac
    31021 ataattggga acgttatgaa ccgaacccat ccataaggta aaatagttaa atgttttcca
    31081 ggtccgctga ctttgcgttt tcggcagtag atcttcgcta tagccgcgtt gctggaatag
    31141 ttttctctga tgttccataa atccctctta atttcacgta tcgaataacg ttgttttaat
    31201 ctagctatga ggaacaaata aaataaaaat attattttta atactgaagc aacatctttg
    31261 gctgctttct ttttttaata ttgctggccg attcattttc tgatatcggc catttttatt
    31321 gcgggtatca ggccagttta tggttagcca ttaattccag cgcctgcact aatgcagagt
    31381 gatccaactg gctgccaccg tttgccgcac aggtattaaa caattcctgg caggtcgcgg
    31441 tgtttggcag gttcagcgcc agtgctttcg cactttgcag tgccaggttg agatctttct
    31501 ggtgcagcgc aattttgaaa cctggattaa aggtgcgttt aatcatgcgc tcaccgtgaa
    31561 cttccagaat acgtgaggaa gcaaagccgc ccatcagcgc ctggcgcaca cgtaccgggt
    31621 ccgcaccggc ttttgaagca aacaacaagg cttcagaaac cgcttcaata ttgagcgcca
    31681 cgataatctg atttgccact ttgcaggttt gaccgtcgcc gttaccgccc acgagagtga
    31741 tatttttacc gagcagttca aacagcggtt ttacacgttc aaatactgct tcatcaccgc
    31801 caaccataat cgataacgtc ccttcacgcg cgccgatttc accgccggag actggcgcat
    31861 cgagataatc gccgcccagt tcattcacct gacgagcgaa acgtttagtt tcaatcgggg
    31921 aaatggagct catatcaaca atggttttgc ccttcagcga ggctttggta caaccatttt
    31981 caccaaacag aacgtcttca acctgtggcg tatccggcac cataataaaa atgatgtccg
    32041 atgcatctgt tacctggcgc gcagtttcaa cactaacggc gcccagtgac agtaattcat
    32101 cagcaaccgg accaatggtt gtgacatgca gttgatgacc ggcacgcgcc agattaatgg
    32161 ccatcggtgt acccataatg cctaagccaa taaatcccag tttcataatt aacctctttt
    32221 aaatttcgct tttcctgaat tcagacaaca cgatcccctg ccaacttagc ggcctaaaaa
    32281 agcattgtct gaatagcgtt acgttttaac ggtacggatc catccagcgt aaaccggctt
    32341 ccgtggtggt ttggggttta tattcacagc caacccaacc gttgtagtca gaattttcaa
    32401 ttaccgtaaa gagatattcg taattaattt ctccggttcc cggttcgccg cgatgcggat
    32461 tatcagcaat ttgcaggtga ccaattttat cggcccactg agtcatggta ttagttaatt
    32521 caccttccat ccgctgcatg tgataaatgt catactgaat tttcaaattg cagcaaccaa
    32581 catcgtcaat cagtttcagt gcctgccggg ttccggtgaa atggaaacca ggaatatcaa
    32641 aatggttaat aggttcaatc agtaataaaa tatcttcttt catcagcata ttcgcagcat
    32701 aacgcaggtt ttctacaagc gttgtgtgaa tctgttcact gctgaaacca gtcggcgttt
    32761 taccgaccag acagttaatt tttttattac ccagagcacg agcataacga atcgctgctg
    32821 ctacgccatc ccgaaactct tcttcacggc caggaataca ggcaataccc cgctcgcccg
    32881 ccgcccagtc acctgccggt aaattgtgca gcgtatgttc gagtttatta ctcgccagca
    32941 cctgttttaa ttcttcaatg tcgtagtcat aagggaacat aaattcaacg ccgcgaaaac
    33001 cacactgcgc agctttctca aaacgggcga gaaaatcata ttcaccaaat aacatcgata
    33061 aattagcaga gaaacgtaac attatttatc tcccttattc atagtgcatg aaacaggctt
    33121 cagtcggtgc atccgctgcg ttatcggcga tatcttcaaa ttccatgacg ttatccagtt
    33181 cactgcccat cgaaatattg gtcacacgct cgagaataac ttccacgact accggtaccc
    33241 gatattgcgc cattaagatt ttcgcctgtt caaaggctgg tgcaatatct tccggtttga
    33301 aaacgcgaat tgctttacaa cctaaacctt ccgctacttt tacgtggtcg acgccataac
    33361 cgttcacttc gctggagtta atattttcga acgcgagctg tacgcagtaa tccatatcaa
    33421 aggcacgttg cgactggcga atcagaccca gataggcgtt attgaccagt acatggatgt
    33481 acggaatgtt gaactgtgcg ccaacggcta attcttcaat caggaactgg aagtcgaagt
    33541 caccggaaat cgccaccaca ttgcgctctg gatcagcggc acaaaccccc agcgccgccg
    33601 gaatcgtcca gcccagcggg cctgcctgcc cacagttgat ccagtggcgg tctttaaaga
    33661 catgcagcat ttgtgccgca gcgatttgtg acaggccaat ggtggtgaca taacaaacat
    33721 cgcggccaaa ggctttgttc atctcttcat acacgcgctg cggtttcacc ggcacgttgt
    33781 cgaagtgggt tttgcgcagc aaagtgcgtt tgcgctgctg gcagtcggcg acccattctt
    33841 tacgacacgg cagacgaccc gctttttgca tctcctgcgc cacttcaacc agcagcgtca
    33901 gcgccgcttt agcatcagag acaatgccga gatccggaca cagcacgcgg ccaatttgcg
    33961 tcggctcaat atcgatatga acgattttgc gcccttcggt gtatttctct accgaaccgg
    34021 tatgacggtt agcaaaacgg ttaccgatac caaacaccat gtcggacgcc agcagcgttg
    34081 cgttaccgta acgatgcgcg gtttgcagac ccaccatccc ggccatcagt tcatgatcgt
    34141 cgggaataca gccccagccc atcagcgttg ggatcaccgg aatgctggtc agttcagcaa
    34201 actgttgtaa cagtgcagct gcgtcagcat tgattacccc gcccccggca acaatcaccg
    34261 gacgttcggc ctggattaac atttctaccg ctttttcgat ctgcatacgg ctggcagcag
    34321 gtttgtagac cggcagcggt tcgtacatgt caggatcaaa ctcgatttcc gcaacctgaa
    34381 cgtcgaacgg taaatccacc agtaccggac ccggacgacc agaacgcatc aggtgaaatg
    34441 cctgttgcag cacgcgaggc accagcgccg cttcacgtac tgtaaccgac attttgctga
    34501 ccggtttagc aattgcttca atatcaacgg cctggaaatc ttctttatgc agacgggcgc
    34561 gcggagcctg gccggtaata cacagaatag gaatggaatc agcagaagca gaatagagcg
    34621 cggtgatcat gtcagtgcct gctggtcccg aggttcccag acatacgccg atattccctg
    34681 ccgttgcgcg ggtataacct tccgccatgt gcgaagcacc ttccacatga cgcgccagaa
    34741 tgtgacgaat accgccgtgc ttacgcatcg ctgaatagaa aggattgatt gctgctcccg
    34801 gaacgccgaa ggcggtggtg ataccttctt tctccagcac atacattgcc gcgtcaacgg
    34861 ctctcatttt tgccatttta ttcctacctc tattaattgg aaaaattttc caactttcaa
    34921 attttgtcaa aaaaacccgc tccgcagagc gggatgcgac agacactatg gatgtgcttt
    34981 cagtcccaac gccgtgctga tatcgcgggc ggtgtctctg accagctcac cctgactgac
    35041 aaaacgatct tctgtcagtc ttgatgaagg cccggagatg gagatagcgg caacaacact
    35101 acctacatca tcgtaaattg ctgaagctat gcaattcagg ccgacaacat gctcttcttt
    35161 atctacggta tagcccagtt cacgcgcttg ttccaggtcc ttcagcaagg tgggcatatc
    35221 cacaagcgta gttggcgtaa actgctgcaa accggtttgc agaatgatgc tcatcaactc
    35281 ctcttccgcc agcggataaa gcagcgcctt gcccgcaccg gaagcatgca gcggcagacg
    35341 actgcccagc ggcgcacaca tcctgaccat cgatttacac tctaactgac caattaatac
    35401 cgcttcattg ccgttacgga tcgcgacatt gaccgtttcg ccggaaagta acatcaggcg
    35461 gcgcataaac ggcccggcga cggagagtac atcgcggtta tggatgtacg ctgcaccgac
    35521 gttaaagaca cctaatccta tatgccacca gcctaactga ctgtcctgat agacaaaatc
    35581 cgctgcctgt aaaaccttca gcaagcgaaa ggtcgtggag agcggcaaat ccagattgag
    35641 agaaatatcg ctaaccgacg aacttccccc acttttttcc agatattgca gaatcgcaat
    35701 tccccgctct aacgcctgtg cgcccttctg tgccacaggc tccgcctgtc ctggcctgcc
    35761 gcgccgtcta acttccgtca tacctacgtt aactccgacc tacaagtgag cataaacaat
    35821 gaacaattcg tttatcttat gcaagtcggt tgcaggcgtc acgcaaaaca gagttcctgt
    35881 tcaggaatac tttcaacatc acagttgtca ctgccgccgc gatcgatggt cagaaaatcg
    35941 gtgacgcgct gccaggcgaa aagtgggtga tgccagacgt tacgatggta attcaccccc
    36001 tgttcaccgt tggtaataaa cgcccgcagc gttgacaggt ctggtttgtc gtcacctaac
    36061 gccacgacta ccacaaacac ttcacctttc atcgggataa acgcctgtgt acccaacgga
    36121 tgacgttcga gttcgtgaat agtcagcggc agattcgccg gttgcgcgcg gttaatgctg
    36181 ataagcgtac ggtcttgctc aagaatctca accagcgcca aatcgtggta acgctctacc
    36241 aggccattgt taatatggaa aaaatcccgt tgctgcgttt cgattacgtc gccataagca
    36301 ctaaaggctt cctgacttaa cggtaatacc tgaagtttca tctttttgtc cttgtaacat
    36361 aattccgttc aaccaccgcc gaccggtcag cttggtttca ataacaggca atctaaaacc
    36421 ttaagagaac aacatcaata caaaagttgg aatatttttc caaaactaga tttagtccac
    36481 aaattgaaca gggttatcat gtaaaaatac tgataaatca gaggataaga aaatcacgca
    36541 acttgcggat ttaaaagggg atacctgaag atgttcgatc cagaaacctt gcggactttc
    36601 attgcggttg ctgaaacagg aagtttttca aaagcggcag aacgattatg taaaaccacg
    36661 gcgacgatca gttatcgcat taaacttctg gaagagaata ccggagtagc gctgtttttc
    36721 cgtacgactc gtagcgtgac gttgacggcg gctggcgagc atctactttg tcaggccaga
    36781 gactggctga gctggctgga aagtatgccc agcgagctgc aacaggtgaa tgatggcgtg
    36841 gaacgccagg tgaatattgt catcaacaac ctgctctata acccccaggc cgtcgcccag
    36901 ttgctggcgt ggctgaatga acgttacccc tttacccagt ttcacatctc ccgacaaatc
    36961 tatatgggcg tctgggattc gctattatac gaaggttttt cactggctat cggcgtcacg
    37021 ggaaccgagg cgctggcaaa tacctttagt cttgatccct taggatcggt gcaatggcgc
    37081 tttgtcatgg cggcggatca tccgctggcg aacgttgaag agccgctaac agaagcacag
    37141 ttgcggcgct ttccggcggt taatattgaa gacagcgccc gcacactaac caaacgcgtc
    37201 gcctggcgat tgccggggca aaaagagatt attgttcccg atatggaaac gaaaatcgcc
    37261 gcccatctgg cgggcgttgg cattggtttt ttgccaaaat cactttgcca gtcaatgatc
    37321 gataatcaac aactggtcag ccgggtaatc ccaacgatgc gccctccttc accattgagt
    37381 ctggcatggc gcaaatttgg cagcggcaaa gcgatagaag atattgtgac cttgtttacc
    37441 cagcgcaggc cggaaatcag cggattttta gaaattttcg gcaacccacg cagttaaaaa
    37501 tcaagtttcc ttgcgcgttg gcgcgtaaaa tagcgcgcaa gctgatttga ggttcacttt
    37561 ttactatgca agagagacac acggaacagg actatcgtgc cctgctgatt gctgatacgc
    37621 ccattattga tgttcgcgcc cccatcgagt ttgagcaagg cgcaatgcct gccgctatca
    37681 acctgccgtt aatgaataac gatgaacgcg ccgccgttgg cacctgctat aaacagcaag
    37741 ggtcagacgc ggcgctggcg ctgggtcata aactggtggc gggtgaaatt cgtcagcagc
    37801 gtatggacgc ctggcgggca gcgtgcctgc aaaatccgca aggtattctc tgctgcgccc
    37861 gtggcggtca gcgctcacat attgtgcaaa gctggttgca tgcagcgggg attgattatc
    37921 cgctggtgaa aggcggttat aaggcactgc gccagaccgc gattcaggcg actattgaac
    37981 tggcacaaaa accgatagtg ctgattggcg gttgtaccgg cagcggtaaa acgctgttag
    38041 tgcagcaaca gccgaacggt gttgatctgg aagggttggc gcgtcatcgc ggttcggcgt
    38101 ttggtcgcac cttacaacca caacttagcc aggcgagttt tgaaaacctg ctggctgccg
    38161 aaatgctaaa aaccgacgcc cgtcagaatt tgcgcctgtg ggtgctggaa gatgaaagcc
    38221 ggatgatcgg ttcgaatcac ctgccggaat gcctgcgcga gcgaatgacc caggcggcga
    38281 ttgcggtagt agaagatccg tttgagatcc gtcttgagcg gctgaacgaa gaatatttcc
    38341 tgcgtatgca tcatgatttt acccacgcgt acggcgacga acagggctgg caggagtatt
    38401 gcgaatacct gcatcacgga ctttcggcga ttaagcgtcg gctggggcta cagcgctata
    38461 acgaactggc tgcaaggctg gatgcggcgc tgacaacgca acttaccacc ggcagcaccg
    38521 acggtcatct ggcctggctg gtgccgttac ttgaagaata ttacgacccg atgtatcgct
    38581 atcagcttga gaaaaaagcg gaaaaagttg tctttcgcgg tgagtgggcg gaagtggcgg
    38641 aatgggttaa gacgcgataa tgagggacgt atttgtatta gtcgcgactt gatctgacac
    38701 atggccttga aaggttgaga gttaccggtt ttgatatgag tgtcgaatcc ttatccaaaa
    38761 caagaggtaa ctctcatgct tcatactgcc aatcccgtca tcaaacacaa agccggtttg
    38821 ctcaatctgg ctgaagaact cagcaacgta tcaaaagcct gtaaaatcat gggcgtttcg
    38881 cgtgatacgt tttatcgtta ccgcgaactg gccgctgaag gcggtgtgga tgcgcagatt
    38941 aatcgtagtc gccgcgctcc taaccttaag aaccgtaccg atgaggcaat tgaacaggct
    39001 gttgttgatt acgccgtcgc tttcccgaca catggtcagc accgggccag caacgagctg
    39061 cgtaaacagg gggtttttat ctccgacagt ggtgtccgtt ctgtctggtt actccataac
    39121 cttgagaatc tcaaaagacg atactgaaca aattttatca ggtgacgttc cgtaaaaagt
    39181 tgtatggagt tctcgatgca gtcggcgcga ctatcagtac ccccgctgaa ccctggacta
    39241 tcgggccgcc gtactggggc atgtcaccct ggcgtgccgc tggttttccg ctaatggttt
    39301 atttcctctt tctttcccgg cgagaattta ttttaatccg gaaggattat ttattttcag
    39361 atagggactc acattaaaca cgtaacattt taattaatgc atggctgaat atgtattaca
    39421 caatgtcaga tatcgtaaaa gaccagtgca attctatgta aactctctgg ctgttcattt
    39481 tatttgttgt ttcagggtcg gaactcaccc tcaataaaca actgaatacg agtagatatt
    39541 catcgtctga gctatatggc tttacacaat agccgacatg tcttttctca gtcagggatt
    39601 atacttttcc tcactacgaa gttgacgact tcgatatggg atagactctt aattcaagca
    39661 attactctgc acaagcatca ggtaacccag ctccaatatt tgttataaaa taatgcgtaa
    39721 cattaatttc accaacaaaa aaaaggttta cgctatcccc ttgccctggt attttaccat
    39781 atttacacca cacttttttt gtatctgtat cattctctga tacgattttc gccacttgtt
    39841 ccctggccct cacagtcaat gttttcttaa acctcccccc atcatcgatt gttagggaaa
    39901 aataagaaaa aaaataatca tcttctacat ttgtggatat tgatatatcc accgctacgt
    39961 gcggaaaaaa atctgggagc catgtaaaaa ccccatcact ttctggtatt gccatatagt
    40021 ttatgtaatg cttacttaca aga
//
LOCUS       DAZGQL010000038        39930 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.038, whole genome shotgun sequence.
ACCESSION   DAZGQL010000038 DAZGQL010000000
VERSION     DAZGQL010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39930
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.038"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(54..1178)
                     /locus_tag="ACHKIR_002271"
     CDS             complement(54..1178)
                     /locus_tag="ACHKIR_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001765453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HFO9990005.1"
                     /translation="MSTLFTINACKTFGCRNLGLASSEDYSWPDYKLGYPALHCRACG
                     SYPPLFDEQQFRDWLSVHLSTWASEKGHFCPVCYGTDMICYGHNPQGSQRIQCRNCKK
                     VWTPKQYQKEITHPQAIETVQLFIPFQGASAVQKLYVLVSLDATRGNILHLSTNYTQH
                     QTGDSLRYSYKGNTEPTMHHRDIVQKVDMREAQFLRRSQFDEIQYGSAVLKRNGKGAI
                     LRPVITAHGHFRILKIRYPDVKTHIISHECFLRGAIITAWADQFRQQQGELWFVEEEI
                     SDSNADTPWHFKGTTYHGWWQNQWQRWEQGNNCKMVCLLTGACLERGANVSLATSRCF
                     ITWLTDQHDFTQSALLSAGRVTQMLTSLALKYNESLTPSC"
     gene            1311..2864
                     /locus_tag="ACHKIR_002272"
     CDS             1311..2864
                     /locus_tag="ACHKIR_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFO9990006.1"
                     /translation="MKIKILAAGIALTLPFWACAKDVTIIYTNDLHAHVEPYKVPWIA
                     DGKRDIGGWANITTLVKQEKAKNKATWFFDAGDYFTGPYISSLTKGKAIIDIMNTMPF
                     DAVTIGNHEFDHGWDNTLLQLSQAKFPIVQGNVFYQNSSKSFWDKPYTIIEKDGVKIG
                     VIGLHGVFAFNDTVSAATRVGIEARDEIKWLQRYIDELKGKVDLTVALIHEGVPARQS
                     SMGGTDVRRALDKDIQTASQVKGLDILITGHAHVGTPEPIKVGNTLILSTDSGGIDVG
                     KLVLDYKEKPHNFTVKNFELKTIYADEWKPDPQTKQVIDGWNKKLDEVVQQTVAQSPV
                     ELKRAYGESASLGNLAADALLVAAGKNTQLALTNSGGIRNEIPAGAITMGGVISTFPF
                     PNELVTMELTGKQLRSLMEHGASLSNGVLQVSKGLEMKYDSSKPVGQRVITLTLNGKP
                     IEDATVYHIATQSFLADGGDGFTAFTEGKARNTTGGYYVYHAVVDYFKAGNTITDEQL
                     NGMRVKDIK"
     gene            3246..4136
                     /gene="metF"
                     /locus_tag="ACHKIR_002273"
     CDS             3246..4136
                     /gene="metF"
                     /locus_tag="ACHKIR_002273"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HFO9990007.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPAELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLRRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            4465..6645
                     /gene="katG"
                     /locus_tag="ACHKIR_002274"
     CDS             4465..6645
                     /gene="katG"
                     /locus_tag="ACHKIR_002274"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HFO9990008.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGGGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            6739..7644
                     /gene="yijE"
                     /locus_tag="ACHKIR_002275"
     CDS             6739..7644
                     /gene="yijE"
                     /locus_tag="ACHKIR_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HFO9990009.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKRI"
     gene            complement(7671..8288)
                     /gene="yijF"
                     /locus_tag="ACHKIR_002276"
     CDS             complement(7671..8288)
                     /gene="yijF"
                     /locus_tag="ACHKIR_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HFO9990010.1"
                     /translation="MKASLALFSLLTVFTSYSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYVQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     AYPQKWQLKRPDSNIDHRRVPNLETWFTRHDKTRPTSKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFAK"
     gene            complement(8564..9667)
                     /gene="gldA"
                     /locus_tag="ACHKIR_002277"
     CDS             complement(8564..9667)
                     /gene="gldA"
                     /locus_tag="ACHKIR_002277"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HFO9990011.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDDGEFDRYLMLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            complement(9678..10340)
                     /gene="fsa"
                     /locus_tag="ACHKIR_002278"
     CDS             complement(9678..10340)
                     /gene="fsa"
                     /locus_tag="ACHKIR_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFO9990012.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVKEAKRLRDAIPGIVVKIPVTSEGLAAIKMLKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            complement(10352..12853)
                     /gene="ptsP"
                     /locus_tag="ACHKIR_002279"
     CDS             complement(10352..12853)
                     /gene="ptsP"
                     /locus_tag="ACHKIR_002279"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFO9990013.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLVGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALGNLPAAKGVDAEQSALE
                     NGLTLVMKNIEFRLLDSDGATSAILEAHRSLAGDTSLREHLLAGVSAGLSCAEAIVAS
                     ANHFCEEFARSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQPAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIPLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLAAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            13162..14241
                     /gene="frwC"
                     /locus_tag="ACHKIR_002280"
     CDS             13162..14241
                     /gene="frwC"
                     /locus_tag="ACHKIR_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HFO9990014.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            14256..14576
                     /gene="frwB"
                     /locus_tag="ACHKIR_002281"
     CDS             14256..14576
                     /gene="frwB"
                     /locus_tag="ACHKIR_002281"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFO9990015.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            14627..16924
                     /gene="pflD"
                     /locus_tag="ACHKIR_002282"
     CDS             14627..16924
                     /gene="pflD"
                     /locus_tag="ACHKIR_002282"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFO9990016.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAASCT
                     DAQRREELLTIAEISRHNAEHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGS
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRSGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            16890..17768
                     /gene="pflC"
                     /locus_tag="ACHKIR_002283"
     CDS             16890..17768
                     /gene="pflC"
                     /locus_tag="ACHKIR_002283"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418387.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HFO9990017.1"
                     /translation="MTSSAGQRISCNVVETRRNDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSQENMQQALDVLIPLNIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVAT
                     MREMAERAGFQVTVGG"
     gene            17770..18111
                     /gene="frwD"
                     /locus_tag="ACHKIR_002284"
     CDS             17770..18111
                     /gene="frwD"
                     /locus_tag="ACHKIR_002284"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFO9990018.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCLLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFDHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(18098..18949)
                     /gene="yijO"
                     /locus_tag="ACHKIR_002285"
     CDS             complement(18098..18949)
                     /gene="yijO"
                     /locus_tag="ACHKIR_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFO9990019.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFIDTGAGAALVPGDVLYVPAGGWNFPQWQAPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQSQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGATGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(19175..20908)
                     /gene="eptC"
                     /locus_tag="ACHKIR_002286"
     CDS             complement(19175..20908)
                     /gene="eptC"
                     /locus_tag="ACHKIR_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFO9990020.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQEVLKDPAPKKLIIVHLLGT
                     HIKYKYRYPEDQGKFDGNTEHVPPGLNAEELESYNDYDNANLYNDHVVASLIKDFKAT
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            complement(21091..23742)
                     /gene="ppc"
                     /locus_tag="ACHKIR_002287"
     CDS             complement(21091..23742)
                     /gene="ppc"
                     /locus_tag="ACHKIR_002287"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HFO9990021.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDVYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GHEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            complement(24044..25195)
                     /gene="argE"
                     /locus_tag="ACHKIR_002288"
     CDS             complement(24044..25195)
                     /gene="argE"
                     /locus_tag="ACHKIR_002288"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HFO9990022.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASCGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLAKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYDAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHKLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELITQVIHHFCWH"
     gene            25349..26353
                     /gene="argC"
                     /locus_tag="ACHKIR_002289"
     CDS             25349..26353
                     /gene="argC"
                     /locus_tag="ACHKIR_002289"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HFO9990023.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVDLPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDVAFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKPGVSQVQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            26361..27137
                     /gene="argB"
                     /locus_tag="ACHKIR_002290"
     CDS             26361..27137
                     /gene="argB"
                     /locus_tag="ACHKIR_002290"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HFO9990024.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            27198..28571
                     /gene="argH"
                     /locus_tag="ACHKIR_002291"
     CDS             27198..28571
                     /gene="argH"
                     /locus_tag="ACHKIR_002291"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HFO9990025.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVESKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKALEDLPLSELQKFSQVIGEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLE"
     gene            29069..30283
                     /gene="manD"
                     /locus_tag="ACHKIR_002292"
     CDS             29069..30283
                     /gene="manD"
                     /locus_tag="ACHKIR_002292"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-mannonate dehydratase ManD"
                     /protein_id="HFO9990026.1"
                     /translation="MKIIAADVFVTCPGRNFVTLKITTASGLCGLGDATLNGRELSVA
                     SYLKDHLCPQLIGRDASRIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYG
                     LAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRLFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHSWRFDEGYMHPGDEPGLGISFDEKLAAKYPYDPAYLP
                     VARLEDGTLWNW"
     gene            30350..31633
                     /locus_tag="ACHKIR_002293"
     CDS             30350..31633
                     /locus_tag="ACHKIR_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9990027.1"
                     /translation="MDVDVSTSVAGNKPQRIRRIQTVTLVLLFMAGIVNFLDRSSLSV
                     AGEAIRGELGLSATEFGVLLSAFSLSYGFSQLPSGILLDRFGPRIVLGAGLIFWSLMQ
                     ALTGMVNSFSHFILMRIGLGIGEAPFMPAGVKSITDWYAQKERGTALGIFNSSTVIGQ
                     AIAPPALVLMQLAWGWRTMFVIIGVAGILVGICWYAWYRNRAQFVLTEEERTYLSAPV
                     KPRPQLQFSEWLALFKHRTTWGMILGFSGVNYTGWLYIAWLPGYLQAEQGFSLAKTGW
                     VAAIPFLAAAVGMWVNGIVVDRLAKKGYDLAKTRKTAIVCGLMMSALGTLLVVQSSSP
                     AQAVAFISMALFCVHFAGTSAWGLVQVMVSETKVASIAGIQNFGSFVFASFAPIVTGW
                     VVDTTHSFNLALVIAACVTFTGALCYFFIVKDRIE"
     gene            31885..32802
                     /gene="oxyR"
                     /locus_tag="ACHKIR_002294"
     CDS             31885..32802
                     /gene="oxyR"
                     /locus_tag="ACHKIR_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HFO9990028.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDDHFDKVLKQAV"
     gene            complement(32785..34185)
                     /gene="sthA"
                     /locus_tag="ACHKIR_002295"
     CDS             complement(32785..34185)
                     /gene="sthA"
                     /locus_tag="ACHKIR_002295"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (Si-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HFO9990029.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            34517..35167
                     /gene="fabR"
                     /locus_tag="ACHKIR_002296"
     CDS             34517..35167
                     /gene="fabR"
                     /locus_tag="ACHKIR_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HFO9990030.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            35167..35526
                     /gene="yijD"
                     /locus_tag="ACHKIR_002297"
     CDS             35167..35526
                     /gene="yijD"
                     /locus_tag="ACHKIR_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HFO9990031.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLL
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(35566..36666)
                     /gene="trmA"
                     /locus_tag="ACHKIR_002298"
     CDS             complement(35566..36666)
                     /gene="trmA"
                     /locus_tag="ACHKIR_002298"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HFO9990032.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IELDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      36780..36970
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            37035..38918
                     /gene="btuB"
                     /locus_tag="ACHKIR_002299"
     CDS             37035..38918
                     /gene="btuB"
                     /locus_tag="ACHKIR_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HFO9990033.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFQQPVNTVLA
                     PTDIVTREDIQRWQSKDLNDVMSHLPGVNISQTGGMGTSSSLYVRGTESRHVLVLIDG
                     VPMARAGIDNAIDISQFPVSLVQRVEYIRGPRSAVYGSGAIGGVVNIITMSNDEKAQI
                     NAGMGTNGYQAYDGVINKRFGDTMVTAAGAYQTTKGFNIQPGSPYSGDSDRDGYRNKL
                     FWGGLQHKFNDNFSGFFRGYSYTANNDYDQGSMYGSATGNEEAQNYNQSWDTGLRYNS
                     GIYSSQLIANYQRLKNYNYTNNLGRYAGDATLDDMEQRYIQWGNSIEVGHGAISGGID
                     WKQEKLTSSSITKSDDYERDTTGLYLTGQQQISNVTLEASGREDHDEQFGWHGTWQTA
                     AGWEFIDGYQATLSYGTSFLSPSLGQQYGAARFASIYGPGIAANPNLKPEESKQWEIG
                     IEGVTGTLDWRLSGYRYEIQNLIDYKSINNVNQYINVKSATIKGLEWTGNITTGPVEH
                     RLTLQYVDPRDDETDKVLYRRAKQQVKYELTGQAYGFEWDVSYQYIGQRYDNAYDETS
                     GSSHTVKLGGVSLWDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGS
                     YTF"
     gene            38863..39720
                     /gene="murI"
                     /locus_tag="ACHKIR_002300"
     CDS             38863..39720
                     /gene="murI"
                     /locus_tag="ACHKIR_002300"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFO9990034.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEEFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         9291 a         9816 c        10831 g         9992 t
ORIGIN      
        1 aaatgaaagg tagtgttctc tcttaattta tggtcgtttg tcgagtgtat tgactaacaa
       61 gatggagtga gcgattcatt gtattttaac gccagtgagg tcagcatttg tgttacgcga
      121 cctgcggata ataacgcgct ttgggtaaag tcgtgttggt ctgtcagcca tgtgataaaa
      181 caacgactgg ttgccagaga aacatttgcg cccctttcta agcaggctcc cgtgagtagg
      241 cagaccatct tgcagttatt cccctgctcc cagcgctgcc actgattttg ccaccaacca
      301 tggtatgtcg ttcccttaaa atgccagggc gtatcagcgt tactgtcact gatttcttct
      361 tctacgaacc aaagttcacc ttgttgttgg cggaactgat ctgcccaggc tgtaataatt
      421 gcgcctctta gaaaacattc gtgtgaaata atatgcgttt tgacatctgg atagcggatt
      481 ttcagtattc tgaaatgccc gtgtgccgtg ataaccgggc gtagtatggc tcccttaccg
      541 ttgcgcttga gtacagcact gccatactga atttcatcga actggctccg gcgcaagaat
      601 tgcgcttcac gcatatctac cttctgcacg atatcgcgat ggtgcatagt tggttctgtg
      661 ttgcccttgt aactgtaccg cagactgtct cctgtctgat gttgcgtata attcgtggag
      721 agatgaagaa tgttgccacg agtggcgtca agactgacta aaacatacag tttttgtact
      781 gcgctggccc cttgaaaggg tatgaacagt tgaacggttt caatggcctg gggatgcgta
      841 atttctttct gatactgttt aggcgtccag acttttttgc agttacggca ttgaatgcgt
      901 tgcgaaccct gtggattgtg accataacaa atcatgtctg tgccataaca taccgggcag
      961 aaatgaccct tctccgaggc ccacgtagat aagtgaactg acaaccagtc acgaaattgt
     1021 tgctcgtcaa acaacggcgg gtaactgcca catgcccgac agtgcagtgc cggataaccc
     1081 agtttatagt ctggccaact gtaatcttcc gatgaggcca atcccaaatt tcggcaaccg
     1141 aacgttttac aggcattaat cgtaaacaga gtcgacatat atcaccaacg atttcacaag
     1201 gtcgttgatt ttgtccggca actatcccct gcagatgtga ttactctcac ataaataagt
     1261 ctttcagaac gtaatgtagc aatcctttat ctcactgaaa gggattgttt atgaaaatta
     1321 aaatacttgc tgcgggtatc gcattaacac tgccgttctg ggcttgcgct aaagatgtca
     1381 ctattattta taccaacgac ctccatgcgc atgtagaacc ttataaagtg ccgtggatcg
     1441 ctgacggtaa acgggatatt ggcggctggg caaatatcac tacgctggtt aagcaagaaa
     1501 aagcaaaaaa taaagcgaca tggttttttg atgcgggcga ctattttacc gggccatata
     1561 tcagcagcct gaccaaaggt aaggctatta tcgatattat gaatactatg ccgtttgatg
     1621 ccgtcacgat aggcaaccat gaatttgacc acggctggga caacacatta ttacagttga
     1681 gtcaggcaaa attcccgatt gtgcagggca atgtttttta tcagaacagc agtaaatcat
     1741 tctgggataa gccctatacc atcatcgaaa aagacggtgt gaaaataggc gttataggtt
     1801 tgcacggtgt atttgccttt aatgacacag tatctgctgc cacgcgagtg ggtattgagg
     1861 cgcgtgatga aattaaatgg ctacaacgtt atatcgatga acttaaaggc aaggttgatt
     1921 tgaccgtcgc cctgatccat gaaggtgttc cggcccgcca gtccagtatg ggaggcacag
     1981 atgtgcgtcg cgcgctggat aaagatattc agacggcaag tcaggtgaaa gggctggata
     2041 ttttgatcac cggacatgca catgtgggta cgccggaacc gattaaagtc ggcaatacgc
     2101 tcatcctctc cactgacagc ggcgggattg atgtgggcaa actggttctc gactacaaag
     2161 agaagccgca taattttacg gtgaaaaact tcgagcttaa aaccatttac gctgacgagt
     2221 ggaagcccga tccacaaacg aaacaggtga tcgacggctg gaataaaaag cttgatgaag
     2281 tggtgcaaca aacggtggcg caatcgccgg ttgaattaaa acgtgcctat ggtgaatcgg
     2341 cttccctcgg gaacctggcg gctgacgctt tactggtagc ggcgggtaaa aacacccaat
     2401 tggcgttaac caactctggc ggcattcgca atgagatccc cgcaggggcg atcacgatgg
     2461 gcggcgtcat cagtacgttc ccgttcccca acgaactggt gacgatggaa ctcacgggta
     2521 aacaattacg cagtctgatg gaacacggcg caagtttaag caatggcgtt ttacaggtat
     2581 cgaaaggcct ggaaatgaag tacgacagca gcaagccggt tggtcagcgg gtaatcacac
     2641 tgactctgaa tggcaaaccc attgaagatg cgacggttta tcacattgct actcagagtt
     2701 tccttgctga tggtggagat ggttttaccg cctttaccga agggaaagcg cgtaacacaa
     2761 cgggcggtta ttacgtttat cacgcggtgg ttgattactt caaagcgggc aacaccatta
     2821 cggatgaaca gctcaacggt atgcgcgtga aggatatcaa ataaaagtgc gcactgaatt
     2881 gactcaactg tttgttaccc gttgattgcg cagatcctgt attcggccta cgcgagggcg
     2941 caggccgaat cagatgcctt agcatcacca tccggtggca ggacgttgtt tttcgaatta
     3001 gcccaggtgt cgctgtgttg tcagaatatg agtcaaaacg tgcgaggtgg attgtgctat
     3061 agagaaatct tttatcgcct ctaattaaga tgagtttttt tcatcttccc tgatttttcc
     3121 tcaccatcat tggttatttt tcggttgacg cccttcggct tttccttcat ctttacatct
     3181 ggacgtctaa acggatagat gtgcacaaca caacatataa cgataagcga ttgatgaggt
     3241 aaggtatgag cttttttcac gccagccagc gggatgccct gaatcagagt ctggcagaag
     3301 tccaggggca gattaacgtt tcgttcgaat ttttcccgcc gcgtaccagt gaaatggagc
     3361 agaccctgtg gaactccatc gatcgcctta gcagcctgaa accgaagttt gtatcggtga
     3421 cctatggcgc aaactccggc gagcgcgacc gtacgcacag cattattaaa ggcattaaag
     3481 atcgcaccgg cctggaagcg gcaccgcatc ttacctgtat tgatgcgacg cctgccgagc
     3541 tgcggaccat tgcacgcgac tactggaata acggtattcg ccatatcgtg gcgctgcgtg
     3601 gcgacctgcc gccgggaagt ggtaagccag aaatgtatgc ttctgacctg gtgacactgt
     3661 taaaagaagt ggcagatttc gatatctccg tggcggcgta tccggaagta catccagaag
     3721 cgaaaagtgc ccaggccgat ttactcaacc tgagacgaaa agtggatgcc ggagccaacc
     3781 gcgccattac tcagttcttc ttcgatgtcg aaagctacct gcgttttcgc gatcgctgcg
     3841 tctcggcagg cattgatgtg gaaatcattc cgggaatttt gccggtttcg aactttaaac
     3901 aggcgaagaa atttgccgac atgaccaacg tgcgtattcc ggcgtggatg gcacaaatgt
     3961 tcgacggcct ggatgacgat gctgaaaccc gcaagctggt gggcgcgaat attgccatgg
     4021 atatggtgaa gattttaagc cgtgaaggag tgaaagattt ccacttctat acgcttaacc
     4081 gcgctgaaat gagttacgcg atttgccata cactgggtgt tcgacctggt ttataaatag
     4141 tgtgcctttt gtgaaaatta cacagtgatc acaaatttta aacagagcac aaaatgctgc
     4201 ctcgaaatga gggcgggaaa ataaggttat cagccttgtt ttctccctca ttacttgaag
     4261 gatacaaatc taaaaccctt ttttataaag catttgtcct aaatcggaca taatcaaaaa
     4321 gtcttaatta cgatcaattt gatctacatc tctttaacca acaatatgta agatctcaac
     4381 tatcgcatcc gtggattaat tcaattataa cttctctcta acgctgtgta tcgtaacggt
     4441 aacactgtag aggggagcac attgatgagc acgtcagacg atatccataa cacgacagcc
     4501 accggcaaat gcccgttcca tcagggcggt cacgaccaga gcgcaggtgg gggcacaacc
     4561 acgcgcgact ggtggccaaa tcaactccgt gttgacctgt taaaccaaca ttctaatcgt
     4621 tctaacccgc tgggtgagga ctttgactac cgcaaagaat tcagcaaatt agattactac
     4681 ggactgaaaa aagatctgaa agccctgctg acagaatctc aaccgtggtg gcctgccgac
     4741 tggggcagct acgccggtct gtttattcgt atggcctggc acggtgcggg tacttaccgt
     4801 tcaatcgatg ggcgcggcgg cgcgggtcgt ggtcagcaac gttttgcacc gctgaactcc
     4861 tggccggata acgtaagcct cgataaagcg cgccgcctgt tgtggccaat caaacagaaa
     4921 tatggtcaga aaatctcctg ggccgacctg tttatcctcg cgggtaacgt ggcgctggaa
     4981 aactccggct tccgtacctt cggttttggt gccggtcgtg aagacgtctg ggaaccggat
     5041 ctggatgtta actggggtga tgaaaaagcc tggctgaccc accgtcatcc ggaagcactg
     5101 gcgaaagcac cgctgggcgc aaccgagatg ggcctgattt acgttaaccc ggaaggcccg
     5161 gatcacagcg gcgaaccgct ttctgcggca gcagctatcc gcgcgacctt cggcaacatg
     5221 ggcatgaacg acgaagaaac cgtggcgctg attgcgggtg gtcatacgct gggtaaaact
     5281 cacggtgccg gtccgacatc aaatgtaggt cctgatccag aagctgcacc gattgaagaa
     5341 caaggtttag gctgggcgag cacttacggc agcggcgttg gcgcagatgc cattacctct
     5401 ggtctggaag tagtctggac ccagacgccg acccaatgga gcaactattt cttcgagaac
     5461 ctgttcaaat atgagtgggt acaaactcgc agcccggctg gcgcaatcca gttcgaagcg
     5521 gtagacgcac cggaaattat cccggatccg tttgatccgt cgaagaaacg taaaccgacg
     5581 atgctggtga ccgacctgac gctgcgtttt gatcctgagt tcgagaagat ctctcgtcgt
     5641 ttcctcaacg atccgcaggc gttcaacgaa gcctttgctc gtgcttggtt caaactgacg
     5701 cacagggata tggggccgaa atctcgttac atcgggccgg aagtaccgaa agaagatctg
     5761 atctggcaag atccgctgcc gcagccgatc tacaacccga ccgagcagga cattatcgat
     5821 ctgaaattcg cgattgcgga ttctggtctg tctgttagtg agctggtatc ggtggcctgg
     5881 gcgtctgctt ctaccttccg tggtggcgac aaacgtggtg gtgccaacgg tgcgcgtctg
     5941 gcattaatgc cgcagcgtga ctgggatgtg aacgccgcag ccgttcgtgc tctgccagtt
     6001 ctggagaaaa ttcagaaaga gtctggcaaa gcttcgctgg cggatatcat cgtgctggca
     6061 ggtgtggttg gcgttgagaa agccgcaagc gccgcaggtt tgagcattca tgtaccgttt
     6121 gcgccgggcc gcgttgatgc gcgtcaggat cagactgaca ttgagatgtt cgaactgctg
     6181 gagccaattg ctgacggttt ccgtaactat cgcgctcgtc tggacgtttc caccaccgag
     6241 tcactgttga ttgataaagc acagcaactg acgctgaccg cgccggaaat gactgcgctg
     6301 gtgggcggga tgcgcgtact gggtgccaac ttcgatggca gcaaaaacgg cgtcttcact
     6361 gaccgcgttg gcgtattgag caatgacttc ttcgtgaact tgctggatat gcgttacgag
     6421 tggaaagcga ccgacgaatc gaaagagctg ttcgaaggcc gtgaccgcga aactggcgaa
     6481 gtgaaataca cagccagccg tgcggatctg gtctttggtt ctaactctgt cctgcgtgcg
     6541 gtggcggaag tctacgccag cagcgatgcc cacgagaagt ttgttaaaga cttcgtggca
     6601 gcgtgggtga aagtgatgaa cctcgaccgt ttcgacctgc tgtaatctga ccttcttcag
     6661 cgactgcctt tcatgcagtc gctgaagttt ctttaccggc gtatagtgtc cacaggaaaa
     6721 ctacacactg gatctctcat gtctgccgca ggaaagagca acccactggc aatcagtggc
     6781 ctggttgtgc tcacacttat ctggagttat agctggattt tcatgaagca agtcaccagc
     6841 tacatcggtg ccttcgactt taccgcctta cgctgcattt tcggcgctct cgttttattc
     6901 atcgtccttt tattacgtgg tcgcggaatg cgcccgacac cgtttaaata caccttagcc
     6961 attgccctgt tacaaacctg cgggatggtc ggcctggcgc agtgggcgct ggtcagcgga
     7021 ggagcgggga aggtggcaat cctgagctat accatgccgt tctgggtggt aattttcgcc
     7081 gcgttgtttc tcggtgaacg cctgcgacgc ggacaatatt tcgcgattct gattgccgct
     7141 ttcggcttat ttttggtgtt gcagccgtgg caactcgatt tctcttcgat gaaaagcgcc
     7201 atgctggcaa tcctctccgg tgtgagttgg ggggcgagcg cgattgttgc taaacgtctg
     7261 tatgcccgcc atccgcgcgt ggatttattg tcgctaacat cctggcagat gctgtatgcg
     7321 gcgctggtga tgagcgtggt cgctttactg gtgccgcaac gtgaaattga ctggcaaccc
     7381 accgtgttct gggcgctggc ctacagtgcg attctggcga cagcgctggc gtggagctta
     7441 tggttgtttg tattgaaaaa cttgcctgcc agtattgcca gcttaagcac actggccgtt
     7501 cccgtttgcg gcgtactctt ttcctggtgg ctgctcggtg agaatccggg ggcagttgaa
     7561 ggtagcggta ttgtgctgat tgtactggca ctggcgctgg tgagccgtaa gaaaaaagaa
     7621 gccgtcagtg taaaaaggat ctgaattttt tcttcatgtg gggagatctc ttatttagca
     7681 aaataccgat agtgccccac catccgccag ctaaacagta catcttcttc ctgcgcgcct
     7741 gcgccaatgt tatgtatcac cagcggcgta ccgtcgcggg cgaagccatc tgacaccacc
     7801 ccaatatgtg ccagcccgtt atctaatcgc caggagacaa tatcgcccgc ttgatagtca
     7861 ctggggttct tgctggtggg gcgtgtttta tcgtggcggg taaaccaggt ttccagatta
     7921 ggcacccggc ggtgatcgat gttgctgtcc gggcgcttta actgccattt ttgcgggtac
     7981 gcagcaaaat tcttcgccat atcttcgtgc accagtttct gcaaatcgac cttctggctg
     8041 cgcaatgcac ggatcaccac atcggaacat acgccgcgtt cttgcgggac atcaccgcca
     8101 ggataggtaa gctgcacata cgccggatcg taaaatagcg tgctgccgat ttgctgtctg
     8161 gcgccgtctg cgatggcaag attggtgttg gcctggattt gtaccacggt tggtggaacg
     8221 gcgggagatt ttaaggagta actggtaaat accgtcagaa gactgaataa cgccagtgaa
     8281 gctttcatct gtttcgtccg tgaaaataag agtcacagat tcgaccttcc gggctaacga
     8341 ttgttctgtg gagaaaatgt ccagaagcgg ataaaaaaac acccgaacaa ggtggaagtt
     8401 tcagattact ggcacagcgt gccggatgcg gcgtgaacgc cttatccggc ctacaaaagt
     8461 acgtaaattt actatattgc agagattgcg taggcctgat aagcgtagcg catcaggcaa
     8521 ttttgcgttc aaactcccgg acaagccggg agtttggagt aggttattcc cactcttgca
     8581 ggaaacgctg accatactgg tcggctacca gcagtgcggc gtaaacctga tctggcgtcg
     8641 cgccgccagg catgttgtgg atggtttcac cttctgcaca tgccgcttct gccacaattc
     8701 gcattttcgc cgggacatct tctttaatat ccagttgagc gagagttatt ggcaaaccta
     8761 ccgcatggct aagtgcagct acggtttcga tttcctccac cggcgcgttt tccagaacca
     8821 gctgcgtcag cgtaccaaat gccacttttt caccgtggta atagtgatgc gcgtccggga
     8881 tagcggtcag gccgttatgc accgcgtgag ccgccgccag cccgccgctt tcaaaaccaa
     8941 cgccgctcaa ataggtgttc gcttcaatca cgcgctccag cgccggggtt actacatgct
     9001 gctcggcggc aagcatcgct ttttcgcctt cttccagcag ggtgttgtag cacagttcag
     9061 ccagtgccag cgccgcctgg gtgcacttgc cgcccgccat ggtggtcgcg ccgctacgag
     9121 agcaagcacg cgcttcaaac caggttgcca gcgcatcgcc gatacccgcc gctaacagac
     9181 gtgcaggtgc gccagcgacg attttggtgt cgacaatgac catattcggg ttatttggca
     9241 acatcagata gcggtcaaat tcaccatcat cggtgtagat aacagacaat gcgctgcacg
     9301 gtgcgtcggt agaggcgata gtcggtgcga tcgctaccgg aacacccatg aaatgtgcca
     9361 gtgctttggc agtatcgaga gtttttccgc caccgatacc gagaattgcg ccacactgcg
     9421 cagtctccgc gatgccacgc agacggtcga tctcattttg cgaacattca ccgccaaacg
     9481 gcgcaatttc tactaccagt ccagcatctt taaagctttt ctcgacagtg gattgagcaa
     9541 aacctaaaac aaatttgtca cccaccacta accagcgttc tgccagcggc ttcaggtatt
     9601 cgcccagacg attaatcaca tcagcgccct ggatgtattt acccggtgat tgaataatgc
     9661 ggtccataat tgctccttta gagatgagta gtgccaaatg cggcattcca gtcgtgttcg
     9721 aacttctcta tagctgactc taccgcaggg gtgttgagca tttgttgcgc tacatctaag
     9781 ggcagggtga tggattcaca tcctgccagt aaacagtcca gcgcctgacg tggtgtttta
     9841 aagctggcag ccagcaccat gctttctggc gcatgcattt ccaacagcgc ttgcagctcc
     9901 tgtacagtac gaatgccgtc accgccctgg gcatctacgc ggttaacgta tggagcaacg
     9961 tatttggctc cagccagcgc cgccagtaat ccttgcgctg cgctgtacac tgcggttccc
    10021 agcgtagtaa tgccttcttt cttcagcatt ttaattgctg ccagaccttc agaggttacc
    10081 ggaattttaa ccacaatgcc cggaatagcg tcgcgcaggc gtttcgcttc tttcaccata
    10141 ccctgcgcgt cgcggctcat ggtctgagca aacagaattc cctcatcacc gatcgctttt
    10201 tgaaggcgcg gcagcacttc ccagatggac tccttgctgg cagcgataat gctcgggtta
    10261 gttgtcaccc cggcaatcgg gaatatgcgt gccagacgtt cgacttctgc gacgttagcg
    10321 gtgtccagat acagttccat gatgtttcct tttacagttc cagttcatgt tgcagcaggc
    10381 tggcgatagc gtcggcagag gcggcgttaa ccagcgcgtt acggaattct tcgtgcatga
    10441 tgcgacgagc gaggcgcgag aaaatgcgca tgtgctgatc gcccgcagca tgtttgttca
    10501 gggttaacat aatgatgaat tgcgcttcat catcgcccca gcgcaccggc gctgccagac
    10561 gcgccacgct gatggtggat tgctcaatgt gttctgattt gctgtgggga atggcaaaac
    10621 taaagcccag accggtagag aaaacggcct cgcgcgccca caagtcggct tccagtttac
    10681 gcggatagcg acagcggccc gccagcagca ggttatcggt catgcctttg agcacttctt
    10741 ctttgctgcg ccagtcgctt tccagtgtga tgcactcggc ggtgaccagc ggtgcgtctt
    10801 gttgggtcat gcggaattgc gccagcaggt gttccacttc cagcgaagta cggcaggcca
    10861 ttgcctggtt gagcaacttg cggcactcac ggctatcaag ttgcgccatc cgcgctttcg
    10921 ccgccggaat tgatggtgcg ctcatgctga gttcatccag ccctaaaccg accagcaacg
    10981 gcagcacgga acctttcgct cccagttcac cgcacagacc aatccatttg ccctggcgat
    11041 gcaccgcctg cacggcgtaa tcgagcgccc gcaagaatgc tggattcagg ctgttgtagt
    11101 gacgagtcac cttagcgtta tcgcgatcca ctgccagtag atattgcgtc aggtcattac
    11161 taccaatact aaagaaatca atctcttcgc agcattgatc gatgatgaac atcaccgacg
    11221 gcacttccag catgatgccg agtggaatct tctcatcaaa cggaatgtgt tcgttacgca
    11281 gttgctgttt ggcttccgcc agtttttctt tcacccataa gatctcttcc attgaggaga
    11341 tcatcgggat catgattttc aggctgccgt gagcagaggc gcgcaggatc gaccgtagtt
    11401 gtgtggtaaa caacgacgcg tattcttcat aaatacgcac cgcgcgataa ccgaggaacg
    11461 ggtttgcctc tgcgggaata ttcagataat caacgggttt gtcaccgcca atatccatag
    11521 tgcgcacaat aatgctgcgt ccgttggcgg actccagcgc ctgacaaaaa atgttgtaca
    11581 gctcgctttc gcccggtgca ctggtgcgat ccatatagag catttcagtg cggaataaac
    11641 caacgccttc cgcaccattg ccgaatgcgg cctgcgcttc cacggagtga gcgatgttag
    11701 cggcaatttc aatgcggata ccgtcagcgg tacgggcttg ttgggtcagc cagacacgct
    11761 gttgctcacg cagggcgtcc tgtacgcggg cttcttgctg ataataacgg gccacggctt
    11821 cccctggctc aaccacaatc gcccctgcgt tgccgtcgat ataaatcgtt tgatgctgcc
    11881 acggagtaag ggcatcaata tccacaccta ccagcgtcgg aatgttgaac gaacgggcaa
    11941 ggatcaccgt atgtgaggtg gtgccgccac ttttcagtaa tagacctttg aggtgatttt
    12001 tatccagttc gaggaactgg ctgggggtca gttcatcagc catacaaatg gcgggctgcg
    12061 tcagtttgcc cggtgccggg aagcgttgct caccgtagat ttgctggagt aactggaagc
    12121 agacgtcgcg tacgtccagg gcacgttctt gcaggtagct gctgctggaa cgggcgaact
    12181 cttcgcaaaa gtgattcgca ctggcaacaa tcgcttcggc gcagcttaat ccggcgctga
    12241 cacctgccag taaatgttcg cgcagggaag tatcgccagc cagggatcgg tgagcttcca
    12301 gtatcgcgct ggtagcaccg tcgctatcca gcagacgaaa ttcaatgttt ttcatcacca
    12361 gtgtcaggcc gttttccagc gcggattgct cggcgtcaac gcctttggca gcaggaagat
    12421 tacccagcgc attgagatct aaagaagaga tcggcgtcag aatgccgcct gcactaccac
    12481 tgcacacggt gcgagcacgg ataatttgcg gattcagatt ggtcagtgaa accggcagtg
    12541 gttccagttc gtcagatttt acttccgcca gcggtgcgtc gcagtggggg aattcatcgc
    12601 gcagccattg gcttaaacgc tggtgcgctt cctgttcgtc ggccccggaa atcagtaact
    12661 ggcagttatc gcccaccagc gtatcggtgc caatcagcgc aagggcgctt ttggcgttgc
    12721 ccttgcggtc agtgcgcagg ttatgccact caatttgtga tgaaaaagta ttacacagcg
    12781 tttcaacgtg gcttgccgga cgtgcatgta cgccgttagg tagctcacaa ataaattcca
    12841 caatcagggc cattgcctct ctcccggtac ggttttctgt ttacaggata agagcgcacg
    12901 gcaacggcct gccatgtgac aaatctgcca aaagctggac aaatgtaatg taaccgtcaa
    12961 tttgcgacgc gtctcacaag acgccgtttt gcggcatgct tccggatcat cgcaagttat
    13021 gaggcggatc gcatttttgt actgatatta caaaagtcca gtaaatggcc tttttatcca
    13081 ctgtttgctc cgcctgcgat tgcctattgt ttcctccatc aaaaatatgg acatgggctt
    13141 ttgcaagccc gggagcagct tatgaatgag ttggtgcaga tcctgaaaaa tacccgtcag
    13201 catttaatga cgggcgtttc acacatgatt cccttcgtgg tatcgggcgg tattttgctg
    13261 gcggtttccg tcatgttgta tggcaaaggc gcagtgccgg atgccgtagc cgatccgaat
    13321 ctgaaaaaac tgtttgatat cggcgttgcg ggtttgacgc tgatggtgcc tttcctcgcc
    13381 gcttacattg gttactccat tgcagagcgt tctgcgctgg ctccgtgcgc tatcggtgcc
    13441 tgggttggta acagctttgg tgcgggcttc tttggtgcac tgatcgccgg gattatcggc
    13501 ggcatcgtgg tgcattacct gaagaaaatt ccggtgcata aagttctgcg ctcggtgatg
    13561 ccaatcttca tcattccgat cgtcggcaca ctgattaccg caggcatcat gatgtggggg
    13621 ctgggcgagc ctgtgggggc attgaccaac agcctgactc agtggcttca ggggatgcag
    13681 cagggcagca ttgttatgct ggcggtgatc atgggcctga tgctggcgtt cgatatgggc
    13741 ggtccggtta acaaagtggc ctatgccttc atgctgattt gcgttgctca gggtgtttat
    13801 accgtggtgg ccattgctgc cgtgggtatt tgtattccgc cgctggggat ggggctggcg
    13861 acgctgattg gtcgtaaaaa tttctccgca gaagagcgcg aaaccggtaa agcggcgctg
    13921 gtgatggggt gcgttggggt tactgaaggg gcgattcctt tcgccgctgc cgatccgctg
    13981 cgtgttattc cttccatcat ggtcggttca gtttgtggtg cagtaactgc ggcgctggtc
    14041 ggtgcgcagt gctatgcagg ctggggcggt ctgattgtgc tgccggtggt tgaaggcaag
    14101 ctgggttata tcgcagcagt agctgtcgga gcagtggtga cggctgtttg tgtgaacgtg
    14161 ctgaaaagtc tggcgcgtaa aaatgggtct tcgactgatg aaaaagaaga cgacctggat
    14221 ttggattttg aaattaatta attgaggaac gagccatgac gaaaattatt gcagtaaccg
    14281 cgtgtccatc tggtgttgcc catacctata tggcggcaga ggcgctggaa agtgccgcga
    14341 aagcgaaagg ctgggaagtg aaagtagaaa cccagggctc gattggtctg gaaaacgaac
    14401 tgactgcgga agacgtggcg agcgccgata tggtgatcct gaccaaagat atcggcatca
    14461 agtttgaaga gcgttttgct ggcaagacca tcgtgcgcgt caacatcagc gacgcggtaa
    14521 aacgcgctga tgccattatg agcaaaattg aagctcacct ggcacaaacc gcttaagttc
    14581 ttatcccgcc cgtaagggcg ggttcgcttt cccacaggag ttcctcatga cgaatcgtat
    14641 ctctcgcctc aaaactgcac tgtttgccaa tacccgtgaa atctcgctgg agcgtgcgct
    14701 gctttatacc gccagccatc ggcaaaccga aggcgaaccg gtgatattgc gccgggcgaa
    14761 agcaacagcg tatatccttg aacatgttga aatttcgatt cgtgatgaag aactgattgc
    14821 cggtaaccgc accgtaaaac cgcgcgccgg gattatgtcg ccggaaatgg acccttactg
    14881 gctgctgaaa gagctggatc aattcccgac gcgtccgcag gaccgctttg ctatcagcga
    14941 agaagataaa cgtatctacc gtgaagagtt gttcccgtac tgggaaaaac gttcgatgaa
    15001 agatttcatt aacgggcaga tgacggatga agtaaaagcc gcgaccagca cgcagatttt
    15061 cagcatcaac cagacggata aaggccaggg gcacattatt attgattacc cgcgcctgct
    15121 gaatcacggg ctgggtgagc tggtagcgca gatgcagcaa cattgtcagc aacagccgga
    15181 gaatcacttt tatcaggcag cgctgttact gctcgaagcc tcgcagaaac acattttgcg
    15241 ttacgccgaa ctggcggaaa cgatggcggc aagctgcact gatgcccagc gtcgcgaaga
    15301 actgctgact attgcggaga tctcccgcca taacgccgaa cataagccgc agacgttctg
    15361 gcaggcgtgc cagttattct ggtacatgaa catcattctg caatacgaat ccaacgccag
    15421 ttcactatcg ttggggcgct tcgaccagta tatgttgccg ttctatcagg catcattaac
    15481 ccagggcgaa gatccggcgt tcctgaaaga actgctcgaa tctttatggg tgaaatgcaa
    15541 cgacatcgtg ctgttgcgct ccaccagtag cgcgcgttat ttcgccggtt tcccgaccgg
    15601 ctataccgca ctgctcggcg ggttaaccga gaacggacgt agcgcggtga acgtgctttc
    15661 gttcctttgc cttgacgctt atcaaagcgt gcaattaccg caaccgaacc tcggcgtgcg
    15721 cactaacgcc ttgatcgaca cgccgttcct gatgaaaacc gcagaaacca ttcgcctcgg
    15781 caccggtatt ccgcaaatct ttaacgatga agtggtggtg ccagcgttcc tcaaccgtgg
    15841 cgtttcgctg gaagatgcac gcgactattc cgtagtgggc tgtgtggaat tatctattcc
    15901 cggcagaacc tacggcttgc atgacatcgc gatgtttaat ctgctgaaag tgatggaaat
    15961 ctgcctgcat gaaaatgaag gcaatgctgc gctgacttat gaaggtttac tggagcagat
    16021 ccgcgccaag atcagccact acatcaccct gatggttgag ggcagcaata tttgcgatat
    16081 cggtcaccgt gactgggcac ctgtaccgct gctctcatcg tttatcagcg attgtctgga
    16141 aaaaggcagc gatattaccg atggcggcgc gcgttataac ttctccggcg tacaggggat
    16201 cggtatcgcc aacctgagcg attctctcca tgcgttgaaa gggatggttt ttgagcaaca
    16261 gcgtttaagt tttgacgaat tgctgtcggt attaaaagcc aacttcgcaa cgccagaagg
    16321 cgaaaaagtc cgtgctcgct taattaaccg cttcgagaaa tacggtaacg atatcgacga
    16381 ggtggataac atcagcgccg aactgttacg ccactactgc aaagaagtgg aaaaatacca
    16441 gaacccgcgc agcggctact tcacgccggg atcgtatacc gtttctgctc acgtcccgtt
    16501 gggatcggtg gttggcgcga cgccagacgg tcgttttgcc ggggaacaac tggcagacgg
    16561 cggcttgtca cccatgctgg gccaggacgc acaagggcca acggcggtac tgaagtcagt
    16621 cagtaagctc gataatacgc tgctgtctaa cggtacgttg ctgaacgtga aattcactcc
    16681 ggcgactctg gaaggtgaag cgggattacg caaactggcc gacttcttac gggcgtttac
    16741 ccagcttaag ttgcagcata ttcagtttaa cgtggtgaac gccgacacgt tgcgggaagc
    16801 gcaacagcgc ccacaagatt atgccgggct ggtggtgcgc gttgccggat acagcgcctt
    16861 ctttgtcgaa ttgtcgaagg agatccagga tgacatcatc cgccggacag cgcatcagct
    16921 gtaacgttgt ggaaacgcgc cgcaatgatg tggcgcgcat tttcaacatt cagcgttatt
    16981 cactgaatga cggcgagggc attcgtacgg tggtcttttt taaaggctgt ccgcatcttt
    17041 gcccatggtg tgctaatccg gagtcgatct ccggcaaaat ccagacggta cgcagagagg
    17101 cgaaatgtct gcactgtgcg aaatgtttgc gtgatgcgga tgaatgcccc tccggggcgt
    17161 ttgaacggat tggccgcgat atcagccttg acgcgctgga gcgggaagtg atgaaagatg
    17221 acattttctt tcgcacgtcc ggcggcggcg tcacgctttc tggcggcgaa gtgttaatgc
    17281 aggcggagtt tgctacccgt tttttacagc ggctgcggtt gtggggcgtg tcatgcgcca
    17341 ttgaaactgc cggagacgcg ccagccagca agctgttacc gctggcgaaa ttgtgcgatg
    17401 aagtgttgtt cgatttaaaa attatggacg cgactcaggc gcgggatgtg gtgaagatga
    17461 atctgccacg cgtgctggag aatctgcgtt tgctggtgag tgagggcgtc aacgtgatcc
    17521 cgcgtttacc gctgatccct ggtttcacgc tcagccagga gaatatgcag caggctctgg
    17581 acgtgctgat cccgctgaat atcaggcaga tccatctgtt accgtttcat cagtacggcg
    17641 aaccgaaata ccgcctgctg gggaaaacat ggtcgatgaa agaggtgcct gcgccgtcgt
    17701 cagctgatgt ggcaacgatg cgcgaaatgg cagaacgggc cggatttcag gttaccgtgg
    17761 gaggttaaaa tggcatacct ggtggcagta accgcctgcg tcagtggcgt ggcgcatact
    17821 tatatggcgg cggaacggct ggaaaagttg tgcctgttag agaagtgggg cgtcagcatt
    17881 gaaactcagg gcgcgctggg aacggagaat cgtttagcgg acgaggatat ccgccgggcg
    17941 gatgttgcgc tgttaattac ggatatcgag cttgccggtg ctgagcgatt tgaccattgc
    18001 cgctatgtgc aatgcagcat ctacgctttc cttcgtgagc cgcagcgggt gatgagcgcg
    18061 gtacgcaaag tgctttctgc gccgcagcaa acccatctca ttctggagta gtcggttttt
    18121 ctgtcagttg gctgtggtac tgccgacgat attccgacgg tgagcgttcg gtatttttac
    18181 gaaacagacg gcagaagtag ttgctgtcga caaagccgca ggcgtgcgcc acttctttta
    18241 ccttcaggtc atagcctttc aataacgtct tagcgtgctc cagtcgcgtg tgattcaggt
    18301 attcgttaaa accagtggcc cccgtttttt gaaacaggtg cgagaggtaa tttggcgaaa
    18361 tatagaatgc ctgtgcgaca gattcgcggg taagcgcgga ggcatagcgt tcgtcgatat
    18421 aatcacgaat agcttcgaac agtgcctggc tgcgtgaggc ggtctggatt tggctgccaa
    18481 gcaaatcgcg gcagtggcta agcaggctgg cgacgataag ccttgccgtt tgctgctcct
    18541 gcgactgcat ttgcatttca ttgagcgttt gtagcagaaa agaacctatg cgtgggccgc
    18601 gtcgggcgac gtgttgcttc gccagatttt gatattgttt gccatcccat tgcacgacgc
    18661 tgaagccgag ttgctgtttg ccaaacagca cgctaaaggt agtggcgggg gcttgccatt
    18721 gtggaaaatt ccagccacca gcgggaacgt acagcacatc gccgggaact aacgctgctc
    18781 cagcgccggt atcaataaac tcaccttcca gcacaatttc cagccgtgga aaatcgacct
    18841 gatacgcaag atcgggcacc gggccgttgc tgctggcaaa gtaaatctgg cgcagggaca
    18901 gcgggccgtt gatcaggcgg gagagcagat agctgacgtc gtgatacatg ttattccctt
    18961 aggtatgtag acgggaaaac agtaactggc attatgaaaa aaggccacct gaaaagtgtg
    19021 gcctgatggg agtgagatgt aaacattgcg gcagtttcag tcgacttgta ttgccggatg
    19081 cggcgtaaac gccttatccg gcctaccgcc gcgatctgta ggccggataa gacgcgtcaa
    19141 gcgtcgcatc cggcaaataa catctggctg ataattactg attacccacc tgatcgccat
    19201 acggcagagt gtcgtaatcg atcagtgcgt ttttcttata cgggttacca atccagcggg
    19261 tagtttcttt gaactgcgga ttcaccactg aacgggttgg atcgtaaccg tcgtaagata
    19321 agcccgccaa atctgaccag gtgtggatca gttccgccag gctgtattta cgatcaacat
    19381 cctgtgagaa atcacgcgga tgagtcgctt gccatttttc tgacgtccac agcaggaacg
    19441 gaatggtgta catatgacgc gtcgggttgt cttcattacg cccctgagtt ttgtgcggcg
    19501 gcgtgtcgta aacctcttca ccgtggtcag agaaataaac caggaaaccg ttcggatcgg
    19561 tcgctttaaa gtctttaatc aggctggcaa ccacgtgatc gttgtacagg ttagcattgt
    19621 cataatcatt atatgactcc agctcttctg cgtttaatcc cggtggaaca tgctctgtat
    19681 taccgtcaaa tttgccctga tcttccggat aacggtactt gtacttgata tgcgtaccca
    19741 gcagatgaac gatgatcagt ttcttcggcg cagggtcctt cagcacttcc tggaacggct
    19801 tcagcacgtt ggtgtcgtat tcacgcgcac tctgcgtacg ttgctggttc atgtagtact
    19861 gcttatcggt ctggcgcgaa aataccgtca gcatggtatt gcgggcggtc atcgtctgct
    19921 ggttggtgat ccagaaggtt ttataacccg cctgtttcat catgttcatc agcgacggct
    19981 gcgtcagata cagatccggg ttcttttcat tggcaaaggt cagcgcctgt tgcaggattt
    20041 caatggtgta cggacgagac gtaaccacgt tattaaacac ggtcagattc ggatcggttt
    20101 tatgcagcgc atccagctcc ggcgtggttt cacgcggata accgtacaga ctcatgcgtc
    20161 cacgctgggt cgattcgcca attaccagca ctaaggtgcg cggttcgtta cccgattcat
    20221 ctttgaaatt cgccagcggc ggcaaggcat tattttcatt cagtagcttt gttagcgagt
    20281 ttagttgctg acgatactga taatagccgg tcaggaattg ccacggtgcg gcaggctcca
    20341 tacgcgatgc caggttatcc aacgttttct caaatggctt gtttttgata aacgtattca
    20401 tggcgatcgg atgcagaatc aagccataaa gtagggcaaa agagacaaca taacgccacg
    20461 gctttggaat atagaccggg cgcaggcgcg tccacagcaa aactgccacc gccgtatagg
    20521 ccagcgcgat aagcacaatc ttcaggctga aatactggct taaatactcg ctggcttcgt
    20581 tggtgttggt ttcaaacatc acaaacagaa cgctctgcga gaactcctga ccgtagatga
    20641 cgtagtagca caacgctgcc agagaggccg cccatagcac cacgccgatc actgcggcaa
    20701 taattttaat ccgcttcgga aagaggaata ccgggatcaa ccacagcgaa ctgaataaca
    20761 gcgagtcgcg aatgccgtta gtgccactat aaccactgat gtaaataatg gcctgtagca
    20821 gagtagagaa aaaccaaaag tagagcagtg cccaacccag ggccttccag ctaaaaaggg
    20881 gtttagcctg gacttctgtg gaatgcatag taagaacctg tcttgaaaaa aatatcgccg
    20941 aatgtaacga caattcctta aggatatctg aagggatatc cagattttga ataaaatgca
    21001 gacagaaata tattgaaaac gagggtgtta gaacagaagt atttcagaaa accctcgcgc
    21061 aaaagcacga gggtttgcag gagaggaaga ttagccggta ttacgcatac ctgccgcaat
    21121 cccggcaata gtgaccatta acgcctgctc gacgcgagga tccggctcat ggccttcttt
    21181 ttctgcctgg cgggagcggt gcagcaactc ggcctgcaat acgttcagcg ggtcggtgta
    21241 aatattccgt agctgaatag actctgcaat ccacggcaga tcggccatca ggtgagaatc
    21301 gttggcaatc gccagcacca ctttgatgtc ttcttcttgc agattgcgta actctttacc
    21361 taacggccac agtgctttgt ctaccaggcg ttggtcatag tattccgcca gccacaggtc
    21421 tgctttggcg aagaccatct ccagcatgcc gagacgcgtc gagaagaatg gccaatcgcg
    21481 gcacatggct tccagctcgc tctgtttgcc atcttcgacc actttttgca gcgccgtacc
    21541 tgcacccagc caggcgggga gcatcaggcg gttttgtgtc caggcaaaaa tccacggaat
    21601 agcgcgcagt gactcaacgc caccggtcgg gcgacgtttt gccggacgtg aacccagcgg
    21661 tagtttgccg agttcctgct ccggcgtggc agagcggaag taaggcacaa aatctttgtt
    21721 ttcacgcacg tagccgcggt agacatcgca tgagataacc gaaagctcat ccataatgcg
    21781 acgccagctc tctttcggct ctggcggtgg cagcaggttg gcttccagaa tcgccccggt
    21841 atacagcgaa aggctgctaa cggtgatttc aggcagaccg tacttgaagc ggatcatctc
    21901 gccctgttcg gtcacgcgca ggccgccttt caggcttcct ggtggttgtg acagcagcgc
    21961 cgcatgggca ggtgcgccgc cgcgaccaat ggaaccgccg cgaccgtgga acaacgttag
    22021 ctcaataccc gctttttcgc aggttttgat taatgcatcc tgtgcctgat attgcgccca
    22081 ggaagctgcc atcacgcccg catcttttgc tgagtcggaa tagccaatca tcaccatctg
    22141 tttgccctga atcaggccgc gataccagtc gatattcagc agctgggtca tgacatcgtt
    22201 ggcgttgttc aggtcatcga gggtttcaaa cagcggagca accggcatcg caaacccgat
    22261 acccgcttct ttcagcagca ggtggacagc cagcacgtcg gacggcgttt tcgccatcga
    22321 gatcacgtag gcggcaatgg agccttgcgg tgcttcggca atcacctggc aggtatcgag
    22381 cacttcgcgc gtttcggcgc ttggttgcca gttgcgcggt agaagcggac gtttggagtt
    22441 cagttcgcgg atcaggaacg cctgtttgtc ggcttctgac cagctctcgt agtcgccgat
    22501 accgaggtag cgggtcagct cgcccagcgc ttcggtatgg cgggtgctct cctgacggat
    22561 atcaatacgg accagcggta cgccgaaaca tttcacgcgg cgcagggtgt cgagcagatc
    22621 gccgttggcg ataataccca tgccacacgc ctgaagtgac tggtagcaag cgtagagcgg
    22681 ttcccacagt tcttcgtttt gtgtcagcag gccttctggt tttggcagtt cttcgccttt
    22741 caggcgcgct tccagccatg cctgtgtcgc catcaggcga gaacgcaggt ttttcatcag
    22801 atagcgatac ggttctgcgg caccttcttc gccaaccagc gccagcagtt caggggtcgc
    22861 ttcaaccatc gacagttcag aaaccagcac ctgaatatct ttcaggaaca gatcggtggc
    22921 tttccagcga ctgagcagca ggacgtggcg ggtgatatcg gcagtgacgt tcgggttgcc
    22981 gtcgcggtca ccgcccatcc acgaggtaaa acggaccgga acaaattcga cgggcagttt
    23041 gtagccgaga ttctcttcca gttgttcgtt cagttcgcgc aggtaattcg gtacgccttg
    23101 ccacaggctg ttttctacta cggcaaagcc ccatttggct tcatctaccg ggcttggacg
    23161 cagcttacgg atttcatctg tatgccatga ctgggcgatc aactggcgca ggcggcgcat
    23221 cagctggtta tgttcgtagt cggcgatatc tttgttatcg agctgtttta agcaggcgtt
    23281 cacttccacc attttgtgga tcagtgtacg acgggtaatt tcggttgggt gagccgtgag
    23341 gaccagttcc agcgacagcg attccactgc ttttttgatg gtgtcttcgc tcagttccgg
    23401 ctggtttttc agtttacgca gggtgcgggc gatcacttcc gggttgctgg cagcttcgcc
    23461 tttcggcgaa atgctgtggt attgctcagc ggtgttggcc aggttcagga actgactaaa
    23521 cgcacgcgca acgggcagca gctcgtcgtt cgacaaattt tgtaaggtgg tgagcaactc
    23581 ctggcggtta gcatcattgc cagcgcgtga agatttcgac aacttacgga tagtttctac
    23641 gcgttcaaga atgtgttctc ccaacgcatc cttgatggtt tctcccagca ctttgccgag
    23701 catactgaca ttactacgca atgcggaata ttgttcgttc atattacccc agacacccca
    23761 tcttatcgtt tgatagccct gtatccttca cgtcgcattg gcgcgaatat gctcgggctt
    23821 tgcttttcgt cgtcttttat aaagccacgt aaaagcggtg acgtcaaatg ctgcgaaatc
    23881 gcttcagcaa acgaataaat agcaggaatt tacgccatta aattcatgac gctttaaata
    23941 agcgtaactt atgggaatgt taaaaaatcg cctcgagtaa caccaaaggt gtaggtcgga
    24001 taagatgcgt aagcatcgca tccgacattg tttggcctac gttttaatgc cagcaaaaat
    24061 ggtgaatcac ctgggtgatc agttcgcggg tgggcttgat aaacctcgtc tccagatatt
    24121 catcaggctg atgagcctga ttaattgagc caggccccag aaccagcgtc gggcataacg
    24181 tttgaataaa cggcgcttcg gtacagtaat tcaccacttc ggtctttgct ccgagcaatt
    24241 tctcaaccac ttcaaccagt ttatgattcg gtgggcattc atagccaggg atcggcggat
    24301 gcagctcgtc gaccgtcaga cgacccggcc agcgttcgct cactggagcc aatgcatcgt
    24361 tgagcaaacc attaagttca ttgagtgtca tgcctggcag cggacgaata tccatatgca
    24421 actcacagca agcacaaata cggttagaag cgtcgccacc gtggatatgc ccgaggttga
    24481 gcgtagggta tggcacggta aacgcgtcgt agtgataacg ttctttcagg ttatcgcgca
    24541 attgcaaaat atgcccgatg gcgtcgtgca tcagttcgat agcgttaact ccgcgtgctg
    24601 gatcgctgga gtggcccgac tggccctgaa tacggatggc gttagagata tgacctttat
    24661 gcgcgcgtac cggttgcagc gacgttggct cgccaatgat ggcgcaatcc gggcgcaggg
    24721 cggtagtttc ggcaaaataa cgcgctccgg ccatactggt ttcttcatcg gcagtcgcca
    24781 gaatgtagag cggttttgca agcttcgtga cgtcgacatc gcgtagcgca tcaaggataa
    24841 acgcaaaaaa gcctttcatg tcggcggtgc ctaagccgta aagcttgccg tcatgctccg
    24901 tcaacgtaaa tggatcgcgc gtccagcgtc cgtcatcaaa tggcaccgta tcggtatgcc
    24961 ccgccagcaa caaaccgcca gccccctgtc cgcaactggc cagcatatta aatttgttgc
    25021 gagttcctgg cacaggctgt acttccacat tgaagcctaa atccttaaac cagtccgcca
    25081 gcagagtgat taaatctgca ttgctttgat cgagtgcctc ttccgtggcg cttattgaag
    25141 gtgtggcaat cagagcacgg taaatctcga taaatggcgg taatttgttt ttcattgttg
    25201 acacacctct ggtcatgata gtatcaatat tcatgcagta tttatgaata aaaatacact
    25261 aacgttgagc gtaataaaac ccaccagccg taaggtgaat gttttacgtt taacctggca
    25321 accagacata agaaggtgaa tagccccgat gttgaatacg ctgattgtgg gtgccagcgg
    25381 ctacgctggc gcagagctag tgacctatgt aaatcgccat ccgcatatga acataaccgc
    25441 tttgactgtt tcagcgcaaa gcaatgatgc gggaaagtta atctccgatt tgcatccgca
    25501 gctaaaaggc atcgttgatc tgccgttgca gccgatgtcg gatatcagcg agtttagccc
    25561 aggggtggac gtggtgtttc tcgccaccgc ccatgaagtc agccacgatt tagcgccgca
    25621 atttcttgaa gcaggctgtg tggtgttcga cctttccggc gcgtttcgtg ttaacgatgt
    25681 cgccttctat gaaaaatatt acggctttac ccatcaatac ccggaactgc tggaacaggc
    25741 agcctacggt ctggcggagt ggtgcggtaa taaattaaaa gaagcgaact tgattgccgt
    25801 gccaggctgt tatccgacgg cagcacagct ggcgctgaaa ccgttgattg atgccgatct
    25861 tcttgacctc aatcagtggc cggtgatcaa cgccaccagc ggcgtgagcg gtgcaggacg
    25921 taaggcagcg atttcaaata gtttttgtga agttagcctg caaccgtatg gcgtctttac
    25981 ccatcgccat caaccagaga tcgccacaca cctcggtgct gacgttatct tcaccccgca
    26041 tctgggcaat ttcccgcgcg gcattctcga aaccattacc tgccgcctga aaccgggtgt
    26101 gagccaggtg caagtggcgc aagcgttaca gcaggcgtat gcccataaac cactggtgcg
    26161 gctgtatgac aaaggcgttc cggcgctgaa aaatgtcgtt ggactgccat tctgcgatat
    26221 cggttttgcc gttcagggcg agcatctgat tattgtggcg accgaagaca acttactgaa
    26281 aggcgcggcg gcacaagcgg tgcagtgcgc caatattcgt ttcggctatg cggaaacgca
    26341 gtctcttatt taagggtgca atgatgaatc cattaattat caaactgggc ggcgtactgc
    26401 tggatagtga ggaggcgctg gaacgtctgt ttagtgcact ggtgaattat cgtgagtcac
    26461 atcagcgtcc gctggtgatt gtccacggtg gcggctgcgt agtggatgag ttgatgaaag
    26521 ggctgaatct gccggtgaaa aagaaaaatg gcctgcgggt gacgcctgct gatcagattg
    26581 acattatcac cggagcactg gcgggaacgg caaataaaac cctgctggca tgggcgaaga
    26641 aacatcagat tgcggccgta ggtttgtttc tcggtgacgg cgacagcgtc aaagtgaccc
    26701 agcttgatga agagttaggc catgttggac tggcgcagcc aggttcgcct aagcttatca
    26761 actcattgct ggagaacggt tatctgccgg tggtcagctc cattggcgta acagacgaag
    26821 ggcaactgat gaacgtcaat gccgatcagg cggcaacggc gctggcggca acgctgggcg
    26881 cggatctgat tttgctctcc gacgtcagtg gcattctcga cggcaaaggg caacgtattg
    26941 ccgaaatgac cgccgcgaaa gcagaacaac tgattgagca gggtattatt actgacggca
    27001 tgatagtgaa agtgaacgcg gcgctggatg ctgcgcgcac gttgggccgt ccggtagata
    27061 tcgcctcctg gcgtcatgcg gaacagcttc cggcactgtt taacggtatg ccgatgggta
    27121 cgcggatttt agcttaagtt ttgttggccg gaggcgcagc tttccggcat tgaatttcaa
    27181 aataaggaaa cagagttatg gcactttggg gcgggcgttt tacccaggca gcagatcaac
    27241 ggttcaaaca attcaacgac tcactgcgct ttgattaccg tctggcggag caggatattg
    27301 ttggctctgt ggcctggtcc aaagccctgg taacggtcgg cgtgttaacc gcagaagagc
    27361 aggcgcaact ggaagaggcg ctgaacgtgc tgctggaaga tgttcgcgcc aggccacaac
    27421 aaatccttga aagcgacgcc gaagatatcc atagctgggt ggaaagcaaa ctgatcgaca
    27481 aagtgggtca gttaggcaaa aaactgcata ccgggcgtag ccgtaatgat caggtagcaa
    27541 ctgacctgaa actgtggtgc aaagataccg ttagcgagtt gctgacggct aaccggcagt
    27601 tacaatccgc tctggtcgag accgcgcaga acaatcagga cgcggtgatg ccgggttaca
    27661 ctcacctgca acgcgcccag ccggtgacgt tcgctcactg gtgcctggcc tacgttgaga
    27721 tgctggcgcg tgatgaaagc cgtttgcagg acgcgcttaa gcgtctggat gtcagcccgc
    27781 taggctgtgg cgcactggca ggaacggcct atgaaatcga ccgtgaacag ttagcaggct
    27841 ggctgggctt tgcttcggcg acccgtaaca gtctcgacag cgtttctgac cgtgaccatg
    27901 tgctggaact gctttctgct gccgctatcg gcatggtgca tctgtcgcgt tttgctgaag
    27961 atctgatttt ctttaacacc ggcgaagcgg ggtttgtgga gctttctgac cgtgtgactt
    28021 ccggttcatc attaatgccg cagaagaaaa acccggatgc gctggagctg attcgcggta
    28081 aatgcggtcg ggtgcagggg gcgttaaccg gcatgatgat gacgctgaaa ggtttgccgc
    28141 tggcttacaa caaagatatg caggaagaca aagaaggtct gttcgacgcg ctcgatacct
    28201 ggctggactg cctgcatatg gcggcgctgg tgctggacgg cattcaggtg aaacgtccgc
    28261 gttgccagga agcggcgcag cagggttacg ccaacgctac tgaactggcg gattatctgg
    28321 tggcgaaagg cgtaccgttc cgcgaggcgc accatattgt gggtgaagct gtggtggaag
    28381 ccattcgtca gggcaaagcg ttggaagatc tgccgctcag tgagttgcag aaattcagcc
    28441 aggtgattgg cgaagatgtc tatccgattc tgtcgctgca atcgtgcctc gacaagcgtg
    28501 ctgcaaaagg cggcgtctca ccgcagcagg tggcgcaggc gattgctttt gcgcaagctc
    28561 ggttagagta gtttgtttat attgctcccc tgaaaatggg gagcttacca cgcctacaaa
    28621 gtgcgacact tcgtaggcgg ataaggcgtt tacgccgcat caggcagaaa tttgtcagta
    28681 ttctggtgac cttaaattta acgcccaacc agaaccatta tttgtcacat tcctttttat
    28741 tcttgcgtct tctttacctt cactctcccc ataaaaatta tattttcaat acatcagcca
    28801 atacatactt taattaattg aatataattc atttctattg ctgtgttcat ttttaatgat
    28861 taatggagtt tgttatgtga ttatttaacg tgttgtttta acgatgatag ctaattattc
    28921 aggtggtttt tgagggtgtt ttttctcctc ggtagaaatt tttattttta atgtgatcat
    28981 ggttgaataa tcgctctttc tctcttgtat ggtagtaggc gcaaaattaa gtttgaaacg
    29041 tctatttaca acacaggaga ttgcaataat gaaaattatt gctgcagatg tttttgttac
    29101 ttgcccaggg cgtaatttcg ttacgcttaa aataaccact gcaagcggac tttgtggatt
    29161 aggagatgcc acgttaaatg gtcgcgaatt gtcggtcgct tcttatctga aagatcatct
    29221 ttgtccacaa ctaattggcc gcgatgccag ccgtatcgaa gatatctggc aattctttta
    29281 taaaggtgct tactggcgac gtggccccgt cacgatgtca gccatttctg ccattgatat
    29341 ggcgttgtgg gatatcaaag caaaagcggc aaatatgcca ttgtatcaac tgcttggtgg
    29401 tgcttctcgt gaaggcgtta tggtttattg ccacaccacc gggcgtacga ttgatgaagt
    29461 ccttgaagac tatgcaaagc accagcagat gggctttaag gcgattcgtg tgcagtgtgg
    29521 tgtaccgggt atgcaaacca cctatggtct ggcgaagggc aaaggactgg cgtatgaacc
    29581 agcaaccaaa ggcctgtggc cagaagagca gttgtggtct tcagaaaagt atctcgattt
    29641 tacgccgaag ttatttgaag cggtgcgtaa taagttcggt tttaacgaac atctgttgca
    29701 tgacatgcac catcgcctga cccccatcga agcggcgcgc tttgggaaga gcattgaaga
    29761 ttaccgcctg ttctggatgg aagatccgac gcctgccgag aatcaggaat gttttcgtct
    29821 gattcgtcag cataccgtga cgccgattgc ggtcggtgaa gtgtttaaca gtatctggga
    29881 ctgcaagcag ttaatcgaag agcagctgat tgactatatt cgcacgacga ttacccatgc
    29941 aggcggcatt accgggatgc gacggattgc cgattttgca tcgctttatc aggtacgtac
    30001 cggttcgcat ggcccttcag atttatcgcc tgtttgccat gcggcggcgc tgcattttga
    30061 cctctgggtg ccaaactttg gcgttcagga gtacatgggc tattccgaac aaatgcttga
    30121 agtcttcccg catagctggc gcttcgatga aggttatatg cacccaggcg atgaaccggg
    30181 gctgggtatt tctttcgatg agaaactggc ggcgaaatat ccgtatgacc cggcgtatct
    30241 ccccgtcgca cgactggaag atggcacatt gtggaactgg taatagcggt tcgcttaatt
    30301 attttctgaa tattcatcaa atcgattaat tccgctcaag gagctaaaaa tggacgttga
    30361 tgtgtcaacc agtgttgcgg gtaataaacc gcaacggatt cgtcgtattc agacagtgac
    30421 cctggtttta ttatttatgg cggggatcgt taattttctc gaccgttcgt cattgagcgt
    30481 ggcaggtgaa gcaattcgtg gcgagttagg attatccgct accgagtttg gtgttttgct
    30541 ttctgcattt tctctgtctt atggtttttc acaacttcct tccggtattt tgctagaccg
    30601 ctttggccca cgaatagtgt taggcgcagg cttaatattc tggtcattaa tgcaggcatt
    30661 aacaggaatg gttaattctt ttagccactt tattttaatg cgtatcggtc tgggtattgg
    30721 cgaagcgcca tttatgcctg cgggagtaaa gtcgatcacc gactggtatg cgcaaaaaga
    30781 acgcggcacg gcgctgggga tttttaactc atctaccgtt atcggtcagg ctatcgcgcc
    30841 tcctgctctt gtattgatgc aactggcatg gggctggcgg acgatgtttg tcatcatcgg
    30901 cgtagcaggg attctggttg ggatctgttg gtacgcgtgg tatcgcaacc gggcgcagtt
    30961 tgtcctgacc gaggaagaac ggacatatct ctccgcgccg gttaaaccgc gtccacagtt
    31021 acaatttagc gagtggctgg cgctgtttaa gcatcggaca acctggggga tgattttagg
    31081 tttctctggc gtcaattata ccggttggct ctacatcgcg tggttacccg gttatttgca
    31141 ggccgaacaa ggtttcagtc tggcgaaaac cggttgggtg gcggcaattc ctttcctcgc
    31201 ggcggcggtc gggatgtggg ttaacggtat tgttgtcgat cggctggcga aaaaaggcta
    31261 tgacctggcg aagacgcgta aaacggcgat tgtctgcggt ttgatgatgt cggcattagg
    31321 cacgttgctg gtcgtgcaat cttcctcgcc agcccaggcg gtagcgttta tctcaatggc
    31381 gctgttctgc gtgcatttcg cgggaacatc tgcatggggg ctggtgcagg tgatggtgtc
    31441 agaaacaaaa gtggcttcca tcgccgggat tcaaaacttt ggcagttttg tttttgcttc
    31501 ctttgctccg atcgtaaccg gttgggtcgt ggataccaca cactcgttta atctggcgct
    31561 ggttatcgcg gcctgtgtga cgttcaccgg ggcgctgtgt tacttcttta ttgtcaaaga
    31621 tcggattgag taaccgttac tatcaggctc tcttgctgtg ggcctgtaga ataaaaaaaa
    31681 gcggatcctg gagatccgca aaagttcacg ttggctttag ttattcgagt tgagaaactc
    31741 tcgaaacggg cagtgacttc aagggttaaa agaggtgccg ctccgtttct gtgagcaatt
    31801 atcagtcaga atgcttgata gggataatcg ttcgttgcta tgctacctat cgccgcgaac
    31861 tatcgtggca atggaggatg gataatgaat attcgtgatc ttgagtacct ggtggcattg
    31921 gccgaacacc gccattttcg gcgtgcggca gattcctgcc acgttagcca gccgacgctt
    31981 agcgggcaaa ttcgtaagct ggaagatgag ttgggtgtga tgttgctgga gcggaccagc
    32041 cgtaaagtgt tattcaccca ggcgggaatg ctgctggtgg atcaggcgcg taccgtgctg
    32101 cgtgaggtga aagtccttaa agagatggca agccagcagg gcgagacgat gtccggaccg
    32161 ctgcatattg gtctgattcc cacggttgga ccgtacctgc taccgcatat tatcccgatg
    32221 ctgcaccaga cctttccaaa gctggaaatg tatctgcatg aagcacagac ccaccagtta
    32281 ctggcgcaac tggacagcgg caaactcgat tgcgtgatcc tcgcgctggt gaaagagagc
    32341 gaagcattca ttgaagtgcc gttgtttgat gagccaatgt tgctggctat ctatgaagac
    32401 catccgtggg cgaaccgcga atgcgtaccg atggccgatc tggcagggga aaaactgctg
    32461 atgctggaag atggtcactg tttgcgcgat caggcaatgg gtttctgttt tgaagccggg
    32521 gcggatgaag atacacactt ccgcgcgacc agcctggaaa ctctgcgcaa catggtggcg
    32581 gcaggtagcg ggatcacttt actgcctgcg ctggctgtgc cgccggaacg caaacgtgat
    32641 ggggttgttt atctgccatg cattaagccg gaaccacgcc gcactattgg cctggtttat
    32701 cgtcctggct caccactgcg cagccgctat gagcagctgg cagaggccat ccgcgcaaga
    32761 atggatgacc atttcgataa agtgttaaaa caggcggttt aagccattta gcgcagctac
    32821 ccgataggct tccgccatcg tcgggtagtt aaaggtggtg ttgacgaagt actcaatagt
    32881 gttgccgcca cctttctgtt ccataatcgc ctgaccgata tgaataattt cggcagcgcg
    32941 ctcgccaaag cagtgaatac ccagaatctc ttttgtttcc cgatggaaca aaattttcag
    33001 cgtgcccacg ttcatgccga cgatttgtgc acgtgccaga tgtttaaact gggcgcggcc
    33061 cacttcatat ggcactttca tcgcggtcag ctgctgttcg gttttgccca cagagctgat
    33121 ttccgggatg gtgtaaatac cggtagggat atcttcaatc agatgtgcgg tggcttcacc
    33181 tttcaccagc gcctgcgcgg caatgcgccc ctggtcatag gccgccgacg ccaggctcgg
    33241 ataaccaatc acgtcgccca ccgcgtaaac gtgcggctgt gcggtctgat acatgctgtt
    33301 gaccttcagc tgtccgcggc tgtcagtttc cagcccaatg ttctgtaacg ccagcgaatc
    33361 ggtattaccg gtgcgaccgt tggcatagag caggcagtca gctttcagtt ttttacccga
    33421 cttcaaatgc atgatcacac cgtcgtcaca gccttcgatc ttctcgtact cttcgttgtg
    33481 acgaatcact acgccactgt tccagaagtg ataggagaga gaatctgaca tctcttgatc
    33541 gagaaatgcc agcaggcgat cgcgggtgtt gatcagatcc acttttacat ccataccgcg
    33601 gaagatcgac gcatattcac agccgatcac tccagcacca tagataagta catggcgcgg
    33661 ttcgtggtgc atgctaagaa ttgagtcgct gtcgtaaatg cgtggatgcg tgaaatcaac
    33721 atctgttgga tgatatggac gagagccgca agcaataaca aatttttcag cggttagtgt
    33781 ctcgacgctg ccgtccgggc aatccagcgc cagcgtgtgc tcgtcaacaa agcgagcgtt
    33841 cccctgcaat atttcacagt gattacgttc gtaaaatccc tgacgcatgc gcgtttgttg
    33901 attaatcacg ttatcggcat ggttaaggat atcggcaaaa gaagagcgga gcagtcgcga
    33961 atggtcgctg taaagtgggt tttgattaaa ttctataatg cggctgacgg cgtgacggag
    34021 agctttcgac gggatggtgc cccagtgggt gcaaccgccg ccaacatttt gataacgctc
    34081 gataactgcg acgcgcgcac cttgcttaac caggcccatt gcagcgcctt cgccgccggg
    34141 gccggaacct attactatgg catcgtaatc gtaggaatgt ggcatggtag ggcttacctg
    34201 ttcttataca taaaagcaac agaatggtaa cattttatcg cgggtaagcc aattgatccc
    34261 cgtcatttat ctggctatat cgtgagcggc ctttgctttg tctgtttctt acttttgacc
    34321 tgaacgtttt attggatttt tatcgacgat actctccgtt taagcggcag gtttccgttg
    34381 tacgtaaaag aaccggccaa agaattgcag taaatatgtt ttattgcgtt accgttcatt
    34441 cacaatactg gagcaatcca gtatgttcat tctctggtat agtgccagca gtacttttgg
    34501 caaggattca gacatcgtga tgggcgtaag agcgcaacaa aaagaaaaaa cccgccgttc
    34561 gctggtggaa gccgcattta gccaattaag tgctgaacgc agcttcgcca gcctgagttt
    34621 gcgtgaagtg gcgcgtgaag cgggcattgc tcccacctct ttttatcggc atttccgcga
    34681 cgtagacgaa ctgggtctga ccatggttga tgagagcggt ttaatgctac gccaactcat
    34741 gcgtcaggcg cgtcagcgta tcgccaaagg cgggagtgtg atccgcacct cggtctccac
    34801 atttatggag ttcatcggta ataatcctaa cgccttccgg ttattattgc gggaacgctc
    34861 cggcacctcc gctgcgtttc gtgccgccgt tgcgcgtgaa attcagcact tcattgcgga
    34921 acttgcggac tatctggaac tcgaaaacca tatgccgcgt gcgtttactg aagcgcaagc
    34981 cgaagcaatg gtgacgattg tcttcagtgc gggggccgag gcgttggatg tcggcgtcga
    35041 acaacgtcgg caattagaag agcgactggt actgcaactg cgaatgattt ctaaaggggc
    35101 ttattactgg tatcgccgtg aacaagagaa aaccgcaatt attccgggaa atgtgaagga
    35161 cgagtaatga aacaagcaaa tcaagataga ggtacgctgc tgctggcgtt ggttgctggc
    35221 ttatcgatta atggtacttt cgcagcgctg tttagctcca ttgtgccatt ttctgtattc
    35281 ccgattattt ccctgttgct gacggtttac tgcctgcatc aacgttatct taatcgcacc
    35341 atgccggtag gcttgccggg tctggcagct gcctgtttta ttctcggcgt actgctgtac
    35401 agcacggtag ttcgtgcgga atatccggat atcggctcta acttcttccc ggcagtactc
    35461 tccgtcatta tggtgttctg gattggcgcg aagatgcgta accgtaagca ggaagttgct
    35521 gagtaatcgg tatcatgccg gatgctggtg catccggcat gggttttact tcgcggtcag
    35581 taatacgccg cactccatat ggtgcgtgta ggggaactga tcaaacagag ccagacgttc
    35641 gaccttgtgc gtctggctta atgtttccag attcttgcat aacgtttccg gattgcagga
    35701 gatatacaga atacgtggat acgcctgcac cattttctcg gtttcactgt ccagaccgct
    35761 gcgcggtggg tcgacaaaaa tggtttcgca ctgataactc tttaagtcga ttccttgcag
    35821 gcggttaaac tcgcgtacgc cattcatcgc ctgagtaaat tcttctgccg ccatacgaat
    35881 aatttgtacg ttatcaatat ggttagcggc aatgttgtat tgcgcggcag cgaccgacgg
    35941 cttagcgatt tcggtggcta atacccgatc aaaattgcgc gccagtgcta atgaaaagtt
    36001 accgttgccg cagtacagct ccagcaaatc gccttttgag cctttggtta cgtccagcgc
    36061 ccattccagc atctgaatat tcatcgccgc gttcggctgg gtaaagctgt tttctacctg
    36121 acggtagatc atctctttcc ctgcgaccgg cagacgttca tcgatgtaat cctggtccag
    36181 ctcgattttg gttttcgttg cccgaccaat cagatgcaca ttcagattct gcgctcgcag
    36241 tgcatcgcgc agggcttccg cttcctgacg ccactcatca tccagcttct tatggtacag
    36301 cagggaaacc accgcctgat tactcagcgt ggtgaggtaa tcaatctgga acaacttgtg
    36361 gcgcagaacg ggattattac gcacacccgc aatcatcgcc gtcatcaact ggttgataag
    36421 ttcactggcg gcggggaagc tatccacgcg gatgcggctt ttggtttgtt gatcgaaaat
    36481 gatgtgatac aggtcatcgc catcgtgcca gatgcggaac tccgcacgca tccggtaatg
    36541 actgaccggc gagcgaaaca cttccggaac caggtcagaa aacggtgcca tcatactttg
    36601 caaacgtacc actttttcgg ctaactgcgc ttcatactgt tctgttggaa ggtgttcggg
    36661 ggtcatgatg tatcctgaaa aattaaagta cgcggcgatt gtagggattg ctcatcagat
    36721 gtccagatct tgatgaattc ctatttgtga gctacgtctg gacagtaact tgttacaacc
    36781 tgtagcatcc acttgccggt cctgtgagtt aatagggaat ccagtgcgaa tctggagctg
    36841 acgcgcagcg gtaaggaaag gtgcgatgat tgcgttatgc ggacactgcc atccggtggg
    36901 aagtcatcat ctcttagtat cttagatacc cctccaagcc cgaagacctg ccggctaacg
    36961 tcgcatctgg ttctcatcat cgcgtaatat tgatgaaacc tgcggcatcc ttcttctatt
    37021 gtggatgctt tacaatgatt aaaaaagctt cgctgctgac ggcgtgttct gtcacagcct
    37081 tttccgcttg ggcacaggat accagcccgg atactctcgt tgttactgct aaccgttttc
    37141 agcagccagt aaatactgtc cttgcaccta ctgatattgt tacccgtgag gatattcaac
    37201 gctggcagtc gaaagacctc aacgatgtta tgagtcatct ccccggtgta aatatctctc
    37261 aaactggtgg aatggggacg agttcttctc tttatgttcg aggcactgaa tcgcgccatg
    37321 ttttagtgtt gattgacggt gtgccgatgg cccgtgccgg tatcgacaat gcaattgata
    37381 ttagccagtt cccggtatca cttgtacagc gtgttgaata tattcgtggg ccgcgttctg
    37441 ccgtttatgg ttctggtgcc atcggaggtg tggtgaacat tattacaatg agtaatgatg
    37501 agaaagctca gatcaacgca ggaatgggta caaatggtta tcaagcctat gatggggtaa
    37561 taaataaacg ttttggcgat acgatggtaa cagcagcagg tgcttaccag acgaccaaag
    37621 gttttaacat tcagcctggc tcaccataca gtggtgatag cgatcgcgat ggatatcgca
    37681 acaaactttt ctgggggggg ctgcagcata agtttaacga taacttctcg gggttcttcc
    37741 gtggttacag ctacacagct aataatgatt acgaccaggg ctcgatgtat ggttctgcta
    37801 ctggcaacga agaagctcag aattacaacc agtcctggga tactggccta cgctataact
    37861 ccggcatcta ttcgtctcag ttaattgcta actatcaacg actcaagaat tataactaca
    37921 caaataatct tggtcgttat gcgggagatg ccaccctgga tgacatggaa cagcgttata
    37981 ttcagtgggg taatagcatt gaggtagggc atggtgcaat tagcggcggg atcgattgga
    38041 aacaggaaaa actgacgtcc agcagcatta caaaatcaga tgattacgaa cgcgatacca
    38101 ccgggttata cctgactgga cagcaacaga tttccaatgt gactttggaa gcttctggcc
    38161 gagaagatca tgatgaacag tttggctggc atggtacctg gcagacagct gctggctggg
    38221 agtttattga tggctaccag gcaacacttt cttacggtac cagttttctt tcgccttctc
    38281 ttggacagca atacggggct gctcgctttg cttcaattta tggtcctggt attgcagcca
    38341 acccaaattt gaagccagaa gaatctaaac agtgggagat tggaattgag ggggtaactg
    38401 ggactctgga ttggcggctc tcaggatatc gttatgaaat tcagaacctg attgattata
    38461 aaagcataaa taatgtcaac cagtacatta atgtgaaatc agccacgatt aaagggctgg
    38521 aatggacggg aaatattaca accggtcctg ttgaacatcg cttgacgtta caatatgttg
    38581 acccgcgcga tgatgaaaca gataaagtgc tgtaccgtcg ggcaaaacag caggttaagt
    38641 acgagttgac tggacaagct tatggtttcg aatgggacgt atcttaccaa tatataggtc
    38701 aacgatacga caacgcttat gacgagacct ctggctcatc acatacagtt aaattaggcg
    38761 gtgtgagctt gtgggatctt gcggttgcgt atccggtcac ctctcatcta acagttcgtg
    38821 gtaaaatagc caacctgttc gacaaagatt atgagacagt ctatggctac caaactgcag
    38881 gacgggaata caccttgtct ggcagctaca ccttctgaac cacgtcccac cgtgctggtg
    38941 tttgactccg gcgtcggtgg gttgtcggtc tatgacgaga tccggcatct cttaccggat
    39001 ctccattaca tttatgcttt cgataacgtc gctttcccgt atggcgaaaa aagcgaagag
    39061 tttattgttg agcgagtggt ggcaattgtc accgcagtgc aagaacgtta tccccttgcg
    39121 ctggctgtgg tcgcttgcaa caccgccagt accgtttcac ttcctgcatt acgcgaaaag
    39181 ttcgacttcc cggttgttgg tgtcgtgccg gcgattaaac ctgctgcacg tctgacggca
    39241 aatggcattg tcggattact ggcaacccgc ggaactgtta aacgttctta tactcatgag
    39301 ctgatcgcgc gtttcgctaa tgaatgccag atagaaatgc tgggctcggc agagatggtc
    39361 gagttggctg aagcgaagct gcatggcgaa gatgtttctc tggatgcgct aaaacgtatc
    39421 ttgcgtccgt ggttaagaat gaaagagccg ccagataccg ttgtactggg gtgtacccat
    39481 ttccctctac tacaagaaga actgttacag gtgctgccag agggaacccg gctggtggat
    39541 tctggcgcag cgattgctcg ccgaacggcc tggctgttag aacatgaagc cccggatgca
    39601 aaatctgccg atgcgaatat tgccttttgt atggcaatga cgccagaagc tgaacaatta
    39661 ttgcccgttt tacagcgtta cggcttcgaa acgctcgaaa aactggcagt tttaggctga
    39721 tttggttgaa tgttgcgcgg tcagaaaatt attttaaatt tcctcttgtc aggccggaat
    39781 aactccctat aatgcgccac cactgacacg gaacaacggc aaacacgccg ccgggtcagc
    39841 ggggttctcc tgagaatctc aacagagaaa agcaaaaaaa tgcttgactc tgtagcggga
    39901 aagcgtatta tgcacacccc gcgccgctga
//
LOCUS       DAZGQL010000039        39917 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.039, whole genome shotgun sequence.
ACCESSION   DAZGQL010000039 DAZGQL010000000
VERSION     DAZGQL010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39917
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.039"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(30..2078)
                     /gene="prc"
                     /locus_tag="ACHKIR_002301"
     CDS             complement(30..2078)
                     /gene="prc"
                     /locus_tag="ACHKIR_002301"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HFO9990035.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQTFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPSGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FGPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            complement(2098..2796)
                     /gene="proQ"
                     /locus_tag="ACHKIR_002302"
     CDS             complement(2098..2796)
                     /gene="proQ"
                     /locus_tag="ACHKIR_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0033592 - RNA strand annealing activity
                     [Evidence IEA];
                     GO_function: GO:0034057 - RNA strand-exchange activity
                     [Evidence IEA];
                     GO_process: GO:0010608 - post-transcriptional regulation
                     of gene expression [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HFO9990036.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            complement(2893..3390)
                     /gene="msrC"
                     /locus_tag="ACHKIR_002303"
     CDS             complement(2893..3390)
                     /gene="msrC"
                     /locus_tag="ACHKIR_002303"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HFO9990037.1"
                     /translation="MNKIEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHVFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            3520..4803
                     /gene="yebS"
                     /locus_tag="ACHKIR_002304"
     CDS             3520..4803
                     /gene="yebS"
                     /locus_tag="ACHKIR_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HFO9990038.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDAITGSMVFFCVIGAPLILVTSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERFY
                     PQRPTTRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            4772..7405
                     /gene="yebT"
                     /locus_tag="ACHKIR_002305"
     CDS             4772..7405
                     /gene="yebT"
                     /locus_tag="ACHKIR_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HFO9990039.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLQEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            7485..8924
                     /gene="rsmF"
                     /locus_tag="ACHKIR_002306"
     CDS             7485..8924
                     /gene="rsmF"
                     /locus_tag="ACHKIR_002306"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HFO9990040.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKTSVADFLQLTAPYGWTLTPIPWCEEGFWIERDSEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNREENEAVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPTPKYKVGNFPFSPVKD
                     REAGQIRQAAASVGLNWDENLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALASPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            9042..9278
                     /gene="yebV"
                     /locus_tag="ACHKIR_002307"
     CDS             9042..9278
                     /gene="yebV"
                     /locus_tag="ACHKIR_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebV family protein"
                     /protein_id="HFO9990041.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            9383..9574
                     /gene="yebW"
                     /locus_tag="ACHKIR_002308"
     CDS             9383..9574
                     /gene="yebW"
                     /locus_tag="ACHKIR_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HFO9990042.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(9575..10231)
                     /gene="pphA"
                     /locus_tag="ACHKIR_002309"
     CDS             complement(9575..10231)
                     /gene="pphA"
                     /locus_tag="ACHKIR_002309"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HFO9990043.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALASQQMSLWFMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     VLWSRSRLGERPKGQGIKGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTLVQLQ
                     "
     gene            complement(10626..10967)
                     /gene="yebY"
                     /locus_tag="ACHKIR_002310"
     CDS             complement(10626..10967)
                     /gene="yebY"
                     /locus_tag="ACHKIR_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HFO9990044.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTKAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(10980..11852)
                     /gene="copD"
                     /locus_tag="ACHKIR_002311"
     CDS             complement(10980..11852)
                     /gene="copD"
                     /locus_tag="ACHKIR_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HFO9990045.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLTKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWSDVFSPNIWLAVLQTQFGGIWLWQIVLAL
                     VTLIVALMQPQNMPRLLFMLTTAQFILLAGVGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMRFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLMLKAILVMIMVVIALANRYVLVPRMRQDEDRAAPWFVWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            complement(11856..12230)
                     /gene="yobA"
                     /locus_tag="ACHKIR_002312"
     CDS             complement(11856..12230)
                     /gene="yobA"
                     /locus_tag="ACHKIR_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HFO9990046.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            12369..12599
                     /gene="holE"
                     /locus_tag="ACHKIR_002313"
     CDS             12369..12599
                     /gene="holE"
                     /locus_tag="ACHKIR_002313"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HFO9990047.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            12701..13357
                     /gene="yobB"
                     /locus_tag="ACHKIR_002314"
     CDS             12701..13357
                     /gene="yobB"
                     /locus_tag="ACHKIR_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein YobB"
                     /protein_id="HFO9990048.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVSAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPEGMDMDPTCSLFTTGQCLGEPDLLASTRR
                     LQFFSHQYSIAVLMANARGNSALWDEHGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            13381..14043
                     /gene="exoX"
                     /locus_tag="ACHKIR_002315"
     CDS             13381..14043
                     /gene="exoX"
                     /locus_tag="ACHKIR_002315"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HFO9990049.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(14040..16100)
                     /gene="ptrB"
                     /locus_tag="ACHKIR_002316"
     CDS             complement(14040..16100)
                     /gene="ptrB"
                     /locus_tag="ACHKIR_002316"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HFO9990050.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPKVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRDNIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPED"
     gene            complement(16309..16968)
                     /gene="yebE"
                     /locus_tag="ACHKIR_002317"
     CDS             complement(16309..16968)
                     /gene="yebE"
                     /locus_tag="ACHKIR_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFO9990051.1"
                     /translation="MANWLNQLQSLLGQSRSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEKGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            complement(17295..17651)
                     /gene="yebF"
                     /locus_tag="ACHKIR_002318"
     CDS             complement(17295..17651)
                     /gene="yebF"
                     /locus_tag="ACHKIR_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HFO9990052.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQPDPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(17718..18008)
                     /gene="yebG"
                     /locus_tag="ACHKIR_002319"
     CDS             complement(17718..18008)
                     /gene="yebG"
                     /locus_tag="ACHKIR_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HFO9990053.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            18142..19320
                     /gene="purT"
                     /locus_tag="ACHKIR_002320"
     CDS             18142..19320
                     /gene="purT"
                     /locus_tag="ACHKIR_002320"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HFO9990054.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADNENLFREAVAEIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAHAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSQLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGAGIQIRLFGKPEIDGSRRLGVALATAESVDEAIERAKHAAGQVKVQG
                     "
     gene            complement(19376..20017)
                     /gene="kdgA"
                     /locus_tag="ACHKIR_002321"
                     /gene_synonym="eda"
     CDS             complement(19376..20017)
                     /gene="kdgA"
                     /locus_tag="ACHKIR_002321"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFO9990055.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(20054..21865)
                     /gene="edd"
                     /locus_tag="ACHKIR_002322"
     CDS             complement(20054..21865)
                     /gene="edd"
                     /locus_tag="ACHKIR_002322"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HFO9990056.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(22100..23575)
                     /gene="zwf"
                     /locus_tag="ACHKIR_002323"
     CDS             complement(22100..23575)
                     /gene="zwf"
                     /locus_tag="ACHKIR_002323"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFO9990057.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFSRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            23913..24782
                     /gene="hexR"
                     /locus_tag="ACHKIR_002324"
                     /gene_synonym="yebK"
     CDS             23913..24782
                     /gene="hexR"
                     /locus_tag="ACHKIR_002324"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HFO9990058.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAIVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            24910..26352
                     /gene="pyk"
                     /locus_tag="ACHKIR_002325"
     CDS             24910..26352
                     /gene="pyk"
                     /locus_tag="ACHKIR_002325"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFO9990059.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(26484..27455)
                     /gene="lpxM"
                     /locus_tag="ACHKIR_002326"
                     /gene_synonym="msbB"
     CDS             complement(26484..27455)
                     /gene="lpxM"
                     /locus_tag="ACHKIR_002326"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFO9990060.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(27575..28897)
                     /gene="mepM"
                     /locus_tag="ACHKIR_002327"
     CDS             complement(27575..28897)
                     /gene="mepM"
                     /locus_tag="ACHKIR_002327"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HFO9990061.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            complement(28913..29845)
                     /gene="znuA"
                     /locus_tag="ACHKIR_002328"
     CDS             complement(28913..29845)
                     /gene="znuA"
                     /locus_tag="ACHKIR_002328"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HFO9990062.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETQVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHDDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLNQLANQYASCLKGD"
     gene            29924..30679
                     /gene="znuC"
                     /locus_tag="ACHKIR_002329"
     CDS             29924..30679
                     /gene="znuC"
                     /locus_tag="ACHKIR_002329"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015633 - ABC-type zinc transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HFO9990063.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRHELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            30676..31461
                     /gene="znuB"
                     /locus_tag="ACHKIR_002330"
     CDS             30676..31461
                     /gene="znuB"
                     /locus_tag="ACHKIR_002330"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HFO9990064.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAIMFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(31611..32621)
                     /gene="ruvB"
                     /locus_tag="ACHKIR_002331"
     CDS             complement(31611..32621)
                     /gene="ruvB"
                     /locus_tag="ACHKIR_002331"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFO9990065.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATVRAWNHFGITPPE
                     MP"
     gene            complement(32630..33241)
                     /gene="ruvA"
                     /locus_tag="ACHKIR_002332"
     CDS             complement(32630..33241)
                     /gene="ruvA"
                     /locus_tag="ACHKIR_002332"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFO9990066.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
     gene            complement(33380..33445)
                     /gene="yobI"
                     /locus_tag="ACHKIR_002333"
     CDS             complement(33380..33445)
                     /gene="yobI"
                     /locus_tag="ACHKIR_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HFO9990067.1"
                     /translation="MCIFITHFFAEYVIFKYLLPI"
     gene            33516..34118
                     /gene="yebB"
                     /locus_tag="ACHKIR_002334"
     CDS             33516..34118
                     /gene="yebB"
                     /locus_tag="ACHKIR_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HFO9990068.1"
                     /translation="MNVNYPAEYEIGDIVFTCIGATLFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGELLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            complement(34120..34641)
                     /gene="ruvC"
                     /locus_tag="ACHKIR_002335"
     CDS             complement(34120..34641)
                     /gene="ruvC"
                     /locus_tag="ACHKIR_002335"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HFO9990069.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(34676..35416)
                     /gene="yebC"
                     /locus_tag="ACHKIR_002336"
     CDS             complement(34676..35416)
                     /gene="yebC"
                     /locus_tag="ACHKIR_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO9990070.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAIDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            complement(35445..35897)
                     /gene="nudB"
                     /locus_tag="ACHKIR_002337"
     CDS             complement(35445..35897)
                     /gene="nudB"
                     /locus_tag="ACHKIR_002337"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HFO9990071.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            complement(36015..37787)
                     /gene="aspS"
                     /locus_tag="ACHKIR_002338"
     CDS             complement(36015..37787)
                     /gene="aspS"
                     /locus_tag="ACHKIR_002338"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFO9990072.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKDENN"
     gene            38097..38663
                     /gene="yecD"
                     /locus_tag="ACHKIR_002339"
     CDS             38097..38663
                     /gene="yecD"
                     /locus_tag="ACHKIR_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFO9990073.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILHAL"
     gene            38660..38755
                     /locus_tag="ACHKIR_002340"
                     /pseudo
     CDS             38660..38755
                     /locus_tag="ACHKIR_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            38981..39229
                     /locus_tag="ACHKIR_002341"
     CDS             38981..39229
                     /locus_tag="ACHKIR_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001217534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFO9990074.1"
                     /translation="MRVEICIAKEKISKMPKGSVPALQQEMLRRVGKRYDDVDVIVKS
                     ASNDGLTILRAVDKESAQEFVQETLQEVWETADDWFIH"
BASE COUNT         9622 a        10300 c         9855 g        10140 t
ORIGIN      
        1 caggcacaat ttcttgtgcc tgattgatat tacttgacgg gagcgggttg ttccgcgggt
       61 ctggcttttt caagcttcgc cagatcgagt gcgatattca ccgtctcatc cagataagga
      121 tccggctcct ggtaatcttt cggtagatca tccagtttct tcaactccgg tttaccttcg
      181 cgtttaaagc gttcgttcaa acgcgccaga cgcgtcgcat catcttcatt attctctttc
      241 tcacgcacag cgtaattcag agaaacgata ttgcgcttgt ccttcatggc gttgaagcgc
      301 gcgatatcct tcatgatgtt ctggaactca ggatctttcg cgatacgcgc attatgttcc
      361 ttcagcagct ccggtccaaa ggccgttaaa tcgcctgatt tcacataggt cgcggcatcg
      421 atgctatccc acggcagcgc gttatcttcg aatttctcac ccgtttccgt ttcttcatta
      481 cccgtcggca tgatgatgtc tggcgttaca cctttacgtt gcgtactgcc gccgttaacg
      541 cgatagaatt tctggatcgt gtactgtaca gatcccagcg ctggccattc aggacgtaac
      601 atctgatcgt agatacggtt caatgaacgg tattgctgaa cggtgccttt accaaacgtc
      661 ggttcaccca caaccagcgc acgaccgtaa tcctgcattg ccgcggcaaa gatttctgaa
      721 gccgaagcac tgaaacggtc aaccagtacc accagcgggc ctttatagaa gacctgtccg
      781 tcggtatcgc tgtcttcacg aactttaccg ttgttatcac ggacctgaac aatcgggccg
      841 gatggaataa acagaccgga gagcgatacc gcttcggtca gcgccccacc accattgcta
      901 cgcaggtcga tgatgacgct gctgacattc tgtttttcca gtttctgcag ttgcactttg
      961 acatcgtctg tcaaacccac atagaagccc ggaatatcca gcacgccgac tttctcttta
     1021 ccgacggtct tcaccgacat tttaaccgcg cggtcttcga ggcgaatacg ttcacgggtc
     1081 aacgttacag tacgggtctt ggtcccttta ccagcaggta aaatttccag acgaacttta
     1141 ctgcccttcg gccctttaat taaggcaacc acatcatcaa gacgccagcc aatcacgtca
     1201 accatcggct tgcctgtttg accaacaccg acaattttgt caccaacgct gatagcttta
     1261 ctcttcgctg ccgggcctcc tgccaccatc gaattgataa cggtgtagtc atcatccatt
     1321 tgcagcactg cgccaatgcc ttccagcgac aaactcattt cagtgttgaa ctgttcggta
     1381 ttacgcgggg aaagatagtt ggtatgcggg tcgatttcac gcgcaaacgc cgtcattgcc
     1441 agcgagaaaa catcttcgct gttggtttgc gccagacgac gaatggcaaa tttgtagcgg
     1501 cgagtcaggg tttcacgaat ttctttatcc gtttttcctg ccagcttcag gcttaactcg
     1561 tcgaatttga ctttactgtc ccacagcgcg ttcaactcag cctcgttttt cggccagggc
     1621 gctttgctgc ggtcaaggtt ataagtgtcg ttgccggtga aatccatcgg cttttccagt
     1681 accgacaaag cgtactggta acgttcaaaa cgacgctttt gcgccagatt gtagagatcg
     1741 tagaaaacgt cgagtttgcc tgaacgcagt tcatcgccta actcggtttt ctttttcgcg
     1801 aactgttcaa catcgcttgc caacagcacg ttgtggctgt aatcgagcag attcaggtag
     1861 cggtcaaaga ttttggccga aaatgtctga tcgaggtcga actggcgata atgagaacgg
     1921 gtgaagcgcg acgttacgcg ctcacttacc gtcgcatgct gcgtctcttc ctttaatacc
     1981 ggaatttgat cagcacgcgt gatatcttct acagcgaagg tctggcctgc tattgcaagc
     2041 aggccagcta acgcggtaag cctaaaaaac atgttcatgc ctggcccggc ctccgtttca
     2101 gaacaccagg tgttctgcgc gcacaatcaa agacataccc gaattcagct ggacgcggac
     2161 gccgtctttg gtgatttcta atacggtggc atccatcgcg ttttgacccg ctttcacctt
     2221 cagggcttgt ccgacagtca gagctgaaat gtcagaaacc ggggtgtgct gttcttcgcg
     2281 aggtgctttt actgtttttg gcgctttctc taccggcttt tgcgcacgag gtttacgttc
     2341 agcgccttct ttgcggcgtg gcgtggtcgg acgtggcttg cgttcgcggc gcggtgcgtc
     2401 ttctttctca ccagcagctg cggcagcttc gcgttttttc gcttgctgtt cagcacgctg
     2461 tgcctgaaca cgcgctttcg cttcttcaag ctgcttgcga gcatgctcta catgttgctc
     2521 gtccagctca ccgcatgggt tgccgtcaag atcgacacgc gttgcgccgg gtttaacacc
     2581 gtaaagataa cgccagctcg aagtgtagag acgtaaagcg gatcgcaatt gcgttttgct
     2641 caggttcatt tccccggcaa cacgatcgac caaatcctga aaaataccga ttttcagcgg
     2701 acgcgcttca ccttccgcac tgaaacagtg gggaaaacgt tcggccagaa acgcgattac
     2761 ttctttactg ctattcaact taggttgatt ttccatgaaa tttcctgatt acaacggacg
     2821 tagccaacaa gcgcaggcat gaacaggcgt cattataatg acgctatcag taaatgctac
     2881 gttatccgtt gattatcctg cgacgctcgc aaagaatttt ttgtaatccg tcgttgcaag
     2941 cactttttca agctgtgcca caagctgacg taagccttgc tcgtcctcgt ctgtaaagcg
     3001 accgaagacg gtactatcga tgtcgagaac accaataatc tgatttttca ccaccagcgg
     3061 cagaacaatt tcagaattac tcgccgcatc acaggcaata tgcccgtcaa acacatgaac
     3121 atcctcgata cgctgcactt gattgcgggc aaccgcagtg ccgcacacgc cgcgcccgac
     3181 gggtatccgg acacaggcaa ttttgccctg aaatggtccg agtaccagtg tatcgtcctc
     3241 aagcaaataa aaacctgccc agtttacgtc agtgagacgc tcatataaca acgcactggt
     3301 gttcgcaagc gttgccagaa aactggtttc tcctgccatc agcgcgttaa agtcgcgatt
     3361 taaatccgcg taaaattcta ttttgttcat tatgtaatca cttggttgtc ttaccaggaa
     3421 ctgccagcct attaaaataa gcattaaatg cgttaatgct caagatcatt ccaatcatgg
     3481 gttaagatta atgttaattc tttttacatt tggcacgtca tggctcttaa cacaccacaa
     3541 attacgccga caaaaaagat aacagtgagg gctatcggtg aggaactgcc gcgtggtgat
     3601 taccaacgtt gcccgcaatg tgacatgctg tttagcctgc ccgagataaa ttctcatcaa
     3661 agtgcctatt gtccgcgctg tcaggcaaaa attcgtgacg ggcgcgactg gtcgctaacg
     3721 cgcctggcgg caatggcctt caccatgctg ttgctgatgc cgtttgcctg gggcgaaccg
     3781 ctgttgcata tctggctgtt aggcattcgt attgacgcca acgtcatgca aggcatctgg
     3841 caaatgacca aacagggcga tgcgataacg ggatcaatgg tctttttttg cgttattggt
     3901 gcccccctca ttctggtgac ctccatagct tatttatggt ttggtaaccg actgggaatg
     3961 aatctacgcc cggtactgct gatgcttgag cgacttaaag agtgggtaat gctggatatc
     4021 tacctggtcg gcattggcgt tgcttctata aaggtacaag attatgccca tatccaggcg
     4081 ggtgtcggct tgttctcttt tgtggcgttg gtgattttaa cgacggtgac gttgtcacat
     4141 ctcaatgtcg aagaactgtg ggagcgattt tatccacagc gccccactac gcgtagagac
     4201 gagaaactcc gtgtctgtct tgggtgccat tttaccggct atcctgatca gcgtggtcgc
     4261 tgcccgcgtt gccatatccc gctacgcctg cgtcgccgtc atagtctgca aaaatgctgg
     4321 gcggcgctgt tagcgtcaat cgttttgttg ttacctgcca acctgttgcc tatttctatc
     4381 atttatctga atggtggccg gcaggaagat acgattcttt ccgggattat gtcgctggca
     4441 agtagcaaca ttgccgttgc gggaatcgtg tttatcgcca gtattctggt accgtttact
     4501 aaagtgatcg tcatgttcac tttactgttg agtattcatt ttaaatgcca gcaaggttta
     4561 cgcacacgca ttctgttact gcggatggtg acctggattg gtcgctggtc gatgctcgac
     4621 ctgtttgtca tatctttaac catgtcgctg attaatcgcg atcagatcct cgcttttact
     4681 atgggaccgg ctgcgtttta tttcggcgca gcggtaattt tgactattct tgctgtggaa
     4741 tggctggaca gccgcttact ttgggatgca catgagtcag gaaacgcccg cttcgacgac
     4801 tgaagcgcag attaaaaata aacgccgtat ctcacctttc tggctgctgc cgttcatcgc
     4861 gctaatgatt gccggttggc tgatttggga tagttatcag gaccggggta ataccgtcac
     4921 catcgacttt atgtcggcgg atggtattgt cccgggccgc acccctgttc gttatcaggg
     4981 cgttgaagtc ggaactgtgc aggatatcag cctgagcgac gatcttcgta agattgaagt
     5041 caaggtcagc atcaagtctg atatgaaaga tgcgctgcgc gaagagacac agttctggct
     5101 ggtgacgcca aaggcctcac tggcaggtgt ctccgggctg gatgccctcg tcggtggtaa
     5161 ctatatcggc atgatgccag gtaaaggtaa agagcaggat cactttgtcg cactcgatac
     5221 ccaaccgaag tatcggctgg ataatggcga tctgatgatc cacctgcaag cccccgatct
     5281 cggttcactg aacagcggtt cattggtcta tttccgtaag atcccggtgg gtaaagtcta
     5341 cgactatgcc atcaatccca ataagcaagg cgtggtgatt gatgtcctga tcgagcggcg
     5401 ttttaccgac ctggtgaaaa aaggtagccg tttctggaac gtttccggcg ttgatgccaa
     5461 cgtcagtatc agtggcgcga aggtgaaact ggaaagtctg gcggcactgg ttaacggtgc
     5521 gattgccttc gattcacctg aagagtcgaa acctgccgag gcggaagata cctttggtct
     5581 gtatgaagat ctcgcccaca gccagcgtgg cgtaataata aaactggaac tgccgagtgg
     5641 ggccggatta accgccgact cgacgccgtt aatgtatcag gggctggaag tcggacagct
     5701 gactaaactg gatttaaatc ctggtggtaa agtcaccgga gaaatgaccg ttgatcccag
     5761 cgtcgttacg ctgttacggg aaaatacccg catcgaatta cgtaatccga aattatccct
     5821 tagcgatgcc aatctcagcg ccctgctgac cggaaaaacc tttgagttgg tgcccggcga
     5881 tggtgagcca cgcaaagagt tcgttgttgt gccaggcgaa aaagccctgc tacaagagcc
     5941 ggatgttctg acactgaccc tgacagcacc agaaagttac ggtattgatg ctggtcagcc
     6001 gctcattctc cacggtgtgc aagtaggcca ggttattgat cgtaaactca ccagcaaagg
     6061 cgttaccttt accgtcgcca tcgagcctca gcatcgcgaa ctggtaaaag gcgatagcaa
     6121 atttgtcgtc aacagccgtg tcgacgtgaa ggtggggctg gatggcgttg agtttctcgg
     6181 tgccagcgcc tcagaatgga ttaacggcgg gatacgtatt ctgccgggcg ataaaggcga
     6241 gatgaaagcc agctatccac tgtatgccaa tctggaaaaa gcgctggaga acagccttag
     6301 cgatttaccc accacaaccg taagtttgag tgcagagacg ctgccggatg tgcaggcagg
     6361 atcggtagtg ctgtaccgta aatttgaagt tggtgaagtt attactgtgc gtccgcgagc
     6421 taacgcgttt gatatcgatc tgcatattaa gccggagtat cgcaaccttc tgaccagcaa
     6481 cagcgtgttc tgggcagaag gtggggcgaa agttcagcta aatggtagtg gtctgaccgt
     6541 acaggcatcc ccgctctcca gagcattaaa gggggctatt agcttcgata acctcagcgg
     6601 tgccagcgcc agtcagcgta aaggcgacaa gcgtattctg tatgcttccg aaacagcggc
     6661 ccgtgcggtt ggcgggcaaa ttacgcttca cgctttcgat gccggaaaac tggcggtcgg
     6721 gatgccaatt cgctatctcg gtattgatat cgggcaaatc cagacgctgg atctgattac
     6781 cgcgcgcaat gaagtacagg caaaggcggt actttacccg gaatatgtcc agacctttgc
     6841 acgcggtggt acacgcttct cagtggtcac accgcaaatt tcggcagctg gcgttgagca
     6901 tcttgatact atcctccagc cgtatatcaa cgtcgaacca ggccggggca atcctcggcg
     6961 cgactttgaa ttacaagagg ctaccataac tgattcgcgt tacctggatg gcttaagcat
     7021 tattgttgaa gcgccggaag ccggttcgtt aggcatcggt acgcctgtgc tgttccgtgg
     7081 tctggaagtc ggtacggtta ccggaatgac gctggggaca ttgtcagatc gcgtgatgat
     7141 tgcgatgcgc atcagtaaac gctatcaaca cctggtgcgt aacaattccg tcttctggtt
     7201 ggcatcgggt tacagtctgg actttggtct gacgggcggc gtagtgaaaa ccggcacctt
     7261 taaccagttt atccgtggcg gcatcgcctt cgccacgcct ccgggtacgc cactggcacc
     7321 gaaagcccag gaaggcaagc acttcctgtt gcaggaaagt gaaccgaaag agtggcgtga
     7381 atggggaact gcgcttccca aataatgccc actgctccgg cgtgcctgcg ccggagcgtt
     7441 tatgctaaac tgcgcgcctg tttttttgcc agtggtatat gctcgtggcc caacacaccg
     7501 tttatttccc ggacgccttt ctgacacaaa tgcgcgaagc gatgccttcg acgctctctt
     7561 ttgatgattt tcttgccgcc tgtcagcgcc cgttgcgccg cagcattcgc gttaatacgc
     7621 tgaaaacctc cgttgctgat ttcctgcaat taactgctcc ttatggctgg acgcttacgc
     7681 caattccgtg gtgtgaagaa ggtttctgga ttgaacgcga cagtgaagat gcattgccgt
     7741 tgggtagtac cgccgagcat ttaagcggcc tgttttatat tcaggaagcc agttcaatgt
     7801 tgcctgttgc cgccttgttt gctgacggta atgcaccaca gcgggtgatg gatgtcgctg
     7861 ccgcgcccgg ctccaaaacg acgcaaattg ccgcgcggat gaataacgaa ggggcaatcc
     7921 ttgccaatga gttttccgcc agtcgggtaa aagtgttaca tgccaatatc agccgctgtg
     7981 gcatcagtaa tgttgcgctc acgcattttg atggccgcgt atttggtgcg gcagtgccag
     8041 aaatgttcga tgccattttg ctggacgctc cctgctccgg cgaaggcgtg gtgcgtaaag
     8101 atcccgatgc gctaaaaaac tggtcaccag aaagcaatca ggaaatcgca gcgacccaac
     8161 gggaactgat cgacagcgcc tttcatgcat tacgtcctgg cggtacgctg gtttactcga
     8221 cctgtacctt aaaccgggaa gaaaacgaag ccgtttgcct gtggctgaaa gagacttatc
     8281 ccgacgcagt agagttttta ccgcttggcg atctcttccc tggtgcaaac aaggcgctga
     8341 ccgaggaagg ctttttgcat gttttcccac aaatttacga ctgcgaaggc ttcttcgttg
     8401 ctcgtctgcg taaaactcag gcgattcccg ccttacccac cccaaaatac aaagtgggca
     8461 atttcccgtt cagcccggtg aaagatcgcg aagccggaca aattcgtcag gcggctgcaa
     8521 gtgttggctt aaactgggat gaaaacctgc gactctggca gcgtgacaaa gaactgtggt
     8581 tgttcccggt gggcattgaa gccctgatcg gtaaagtccg attttctcgc ttggggatta
     8641 aacttgccga aacgcacaac aaaggttatc gctggcagca tgaagccgtt attgcccttg
     8701 cctcccccga caatgtgaac gcttttgaac tgacaccgca ggaagcggaa gagtggtatc
     8761 gcgggcgcga tgtttacccg caagccgcgc cagtagcgga tgatgtattg gttactttcc
     8821 agcatcagcc gattggttta gccaaacgga ttggttcgcg actgaaaaac agctacccgc
     8881 gtgaactggt gcgcgatggg aaacttttta ccggtaacgc ctgacagcgc acaaaaaaag
     8941 cgcacttttt gactggcaca ttcggctgcc tcaactaggc tgaaaaatgg tgcgatcgga
     9001 ctggtcgtac cacaaccggc agctaaatgg agagcacgaa gatgaaaacc agtgtgcgca
     9061 taggcgcttt tgaaatcgac gacggcgaat tacacggcga atcgccgggt gatcgaacgt
     9121 taaccattcc ttgtaaatca gaccccgatt tatgtatgca actggatgcc tgggatgctg
     9181 aaaccagtat tcctgccctg ctaaatggcg aacactctgt cctttaccgt acccgttacg
     9241 atcaacaatc tgatgcctgg attatgcgtc ttgcctgatc caaaaagaac ccgtcggcat
     9301 ggcgggttat ttgtcctggt tatttccccg ttgtaaaatc tctcctaaac ttaacggtac
     9361 ggcaccacac ttcggggatg aaatgttcgc gctggtactt tttgtttgct acctggatgg
     9421 cggttgtgaa gatattgttg tggatgtcta caacacggaa cagcagtgtc tttattctat
     9481 gagcgatcaa cggatccgcc atggcggttg ttttccaatc gaggatttta ttgatggttt
     9541 ctggcgacca gcacaggagt acggtgattt ttaattattg caattgcaca agagtcagtt
     9601 cgcccccaaa gacagcaccg gtatcaatat aatgcaggtt gccaatatcc acgcgatgtc
     9661 gcaacggtgt atgaccaaac cagaaatgat cagcaccttt aattccctgc ccttttgggc
     9721 gttcacctaa tcgcgagcgg ctccacaaga cctgatgcaa atcaacgtcc ttttgccatt
     9781 cataaacatc atccggataa tcggcatggg caataacatg tttgccggtg cgactgtgta
     9841 cttcaagaat aaagggcaaa tgctgacatt tttccagcgc cgttttcgct tgtttctgtt
     9901 gattatctgc cagcgcaata aaccagtcgc cgccattcat gaaccacaaa gacatctgct
     9961 gggatgccag cgcatccatc gccatctgtt catgattgcc tcttaccgca cgaacccaat
    10021 gttgttccag taactgcagg caacgtaaac tttgcggccc acgatcgata acgtctccca
    10081 ctgagataag taaatctcgc cacggatcaa aacgacaatg ccataatttg cggcgcaact
    10141 gctcaagaca accgtgtata tcgcctgaaa gccagatatg tcgccattga tgacccgcaa
    10201 ttctctgata aacgggcgca ggctgtttca tcaatatttt cctcccgcgc taaagatcac
    10261 ataatcttaa caagaatgtt aaaaaacgct ggacgcagac agtagagtgt gtgttatggt
    10321 tgactataaa gtcagcgaag ggaatgcttc tggcttttaa cagataaaaa gagaccgaac
    10381 acgattcctg tattcggtcc agggaaatgg ctcttgggag agagccgtgc gctaaaagtt
    10441 ggcattaatg caggcttagt tgccttgctc tttaagaata gatgacgacg ccaggttttc
    10501 cagtttgcgt gcaaaatggt caataaaaag cgtggtggtc atcagcttaa atgttaaaaa
    10561 ccgcccgttc tggtgaaaga actgaggcgg ttttttattg gaaatcaaaa ggctatttta
    10621 ggtaattaac agagtttttc agctcgctct ataaacggtg ccagactcat tttttcgccg
    10681 ggattgttag gatcatcaat ctgaatcacc gaaatgggtt gggctttagt cttcccactg
    10741 gcaacttcct tttgtgcgat atcgtttaaa ggatactgca cgagggtact tgggttgatg
    10801 acatacaaag cattacccgg tcggcaagtc agcatcacct cttcgcgatt aaacgcccat
    10861 ttgtctttac ccacttcaaa acggctgacg gtaatcacct gcggtgccgc cagcgccgct
    10921 gcagaactgg tgagtaacag aaacgccaga atactttttt tcatcatttt cgtaatccat
    10981 caaaaaggtt cgagagtcgc aagcaggctg atgatcacca gcactaccgc acctatcgcc
    11041 cattcgagtt tagtcatcca cacaaaccag ggagccgcac gatcttcatc ctgcctcatg
    11101 cgcggtacaa gaacataacg attagccagc gcgatgacca ccataatcat caccaggatg
    11161 gctttgagca ttaagagttg gccccagtaa gtcgtaagcg tggggggaaa tccggtaatc
    11221 aacaatgcat taagcacgcc gcttgccagt acgccgatca ccgcaaaatg cccgcaccag
    11281 gaaaaacgca tcagcgcctg aatagcctgg tgtcgccaac ggcctttgat gagttgcata
    11341 caccagagca ccggcagtaa tcccccaaac cacgccgccg cacagatcag gtgaattgcg
    11401 tgattagtct gatggatttt cgccgttacc ccttcattca gcgtcgcatg cccgactccc
    11461 gccagcagaa taaattgcgc ggtagtgagc ataaatagca aacgtggcat attctgcggt
    11521 tgcataaggg caacgatcaa cgttacgagg gcgagaacaa tttgccataa ccagatgcca
    11581 ccaaactgcg tttgcaaaac agccagccaa atgtttggcg aaaatacatc tgaccatccc
    11641 gttcccatca acccgccctg aacggcgagc attgcagtgg cactgatcaa actccagacg
    11701 gcagcgtgct gttgtaaacg cagaaaacgc ttcgtcagca agcgacgaat cgttaagggg
    11761 gccagccagg caccgtacat tgcgaagcca aaaaccagca tcaacgaggt gaaatggata
    11821 aatcgcagcg cgatccaggt aaacgccagc atgatttatt tcacgctaaa ggtgtagtgt
    11881 cctttcgttt tatgcccgtc caccgaaaca acatgccagt caacggtgta ggttccgggt
    11941 ttcagcgaat cggccagtgg aacaatcagt tgcttttggt cctgctcatt ccgcttcgcc
    12001 ggtaatgttt taatattttc gttttttggc cccgtgattt ttgcaccact gaatcctgtt
    12061 tcaacgcctt ccgagaagtt taaagtgatt gcctgcgggg cagctgtcac ttgcgcattt
    12121 gccgcaggat actgatgcgt taaatgcgca tgtgcccaga cggaaggtgt caccaacgaa
    12181 gtggtcagga ttgccagcgc gtagcgaagg gagcgtgcag ttgaagccat attatctatt
    12241 cctttttgta ataacttttt tacagagcat aaccttgtct aatgtctgag tcgaggatca
    12301 tcaattccgg cttgccatcc aggctcactc ttagtaactt ttgcccgcga atgatgagga
    12361 gattaagaat gctgaagaat ctggctaaac tggatcaaac agaaatggat aaagtgaatg
    12421 tcgatttggc ggcggccggg gtggcattta aagaacgcta caatatgccg gtgatcgctg
    12481 aagcggttga acgtgaacag cctgaacatt tgcgcagctg gtttcgcgag cggcttatcg
    12541 cccaccgttt ggcttcggtc aatctgtcac gtttacctta cgagcccaaa cttaaataaa
    12601 acttatacag agttacactt tcttacataa cgcctgctaa attatgagta ttttctaaac
    12661 cgcacccata atttgcagtc attttgaaaa ggaagtcatt atgtcgttct ggaaagttgc
    12721 agcagcgcaa tatgagcctc gtaaaacctc actgaccgaa caggtagctc atcatctgga
    12781 gtttgtgagt gccgccgcca ggcaacaatg tcagctatta gtttttccat cgctttcttt
    12841 actggggtgt gattattcgc gacgtgccct tcctgcccca cccgatctct cgctgttaga
    12901 cccgctttgt tatgctgcaa cgacctggcg aatgaccatc attgccggcc ttcccgttga
    12961 atataacgat cgctttattc gtggaattgc ggtgttcgca ccgtggcgaa aaacaccagg
    13021 gatctaccat cagagtcatg gtgcatgtct gggccgtcgt tccagaacca tcacggtggt
    13081 cgatgaacaa ccggaaggta tggatatgga cccgacctgt tcactgttta cgacagggca
    13141 atgtctgggg gaacccgacc tactggcatc tacccgccgc ttacaatttt tttcacatca
    13201 gtactcaatt gccgtgctga tggcaaatgc ccgtggtaac agtgcactat gggatgaaca
    13261 tggtcgtctc atcgttcgcg ccgatcgtgg ttcattattg ttagtcggtc agcgttcatc
    13321 acagggttgg caaggcgata tcattccatt acgctaggct ttttcggcct ggagcatgcc
    13381 atgttgcgca ttatcgatac agaaacctgc ggtttgcagg gagggatcgt tgagattgcc
    13441 tctgttgatg tcattgacgg aaaaatcgtc aaccccatga gccacctggt gcgccccgat
    13501 cgtcctatta gtccacaagc gatggcgatt catcgcatca ccgaagccat ggtcgccgat
    13561 aaaccgtgga ttgaagatgt gatcccacac tattacggta gtgaatggta tgtcgcgcac
    13621 aacgccagct ttgaccgccg cgtactgcct gagatgcccg gtgagtggat ttgcactatg
    13681 aaactggccc gtcgtttgtg gccagggatc aagtacagca atatggcgtt atataaaaca
    13741 cggaagctca atgtacagac gccgccgggc ctgcatcatc accgcgcgtt gtatgactgt
    13801 tatatcaccg ctgcgttgct tatcgatatt atgaacacct ccggctggac ggcagaacag
    13861 atggccgata tcaccggacg tccgtcgttg atgacgacct tcacctttgg caaataccgt
    13921 ggcaaagcgg tttccgacgt tgccgaacgc gatccgggct atctgcgctg gttgtttaat
    13981 aacctggaca gcatgagccc ggagctgcgt ttaacactga aacattatct ggaaaatact
    14041 taatcctcag gcgtagcggg taatgttccc tgcgccagcg cgaccagaaa agcatattcc
    14101 atcgctacgc cttcgtacga tttaaagcga ccagatttac cgccatgacc tgagtccatg
    14161 tcggtacaga gcaataaaag atggtcatcg gttttcagct cgcgcaattt agcgacccat
    14221 tttgccggtt cccaatattg cacctgagaa tcgtgcaaac cggtcgttac cagtaaatgc
    14281 ggataagcct gtgcggtgac gttgtcatat gggctgtagc ttttcatgta ctcgtaatat
    14341 tgcggatcct gcggattccc ccactcttca aactcaccag tggtaagagg aattgattca
    14401 tcaagcattg ttgttacaac atcaacaaac ggtacctggg cgataacgcc gtggaataat
    14461 tccgggcgtt gattaattgc aacgcccatc aacatgcccc ccgcacttcc gcccatcgca
    14521 taacagagcg aaggagagcc atagcccagt tttaacaatg catcgcaggc atcaagataa
    14581 tcattaaacg tatttttctt cttcagaaat ttaccgtctt cgtaccattg ttgccccagc
    14641 tcaccaccgc cgcgaacatg gacaatggcg tagacaaagc cacgatctaa caaactcaag
    14701 cggctaaaac tgaaatcggc atcaatactt gcgccgtaag aaccgtagcc atacaccagc
    14761 agcgggttgt gtcctttgcg aaaatgtttg cgatgataga ccagcgaaac cggaacttcg
    14821 acgccatcac gggcgactat ccacaggtgt tcactgcggt aattcgccgc atcaaaacca
    14881 ggaacttccg tttgttttaa tacacgacgc tcaccggtat ccatatccag ttcaaacaaa
    14941 gtatctggtg tggtcatgga agaataacca taacgcaatc gcgcggtttc aggttctgga
    15001 ttgtaggcaa tccaggtcac ataggccgga tcatcaaagg caatgccaat gacttcccgg
    15061 gtcttgcggt tgatttggcg caaactggtt aacccgcgct gacgctcttc aaccaccagc
    15121 cagtcggtaa acagcgtaaa cccttccagc ataatgttat cacgtggcgg aattaactct
    15181 tcccactgtt gctcatcacg catacgggta cggtataagc caaagttttt accgtggcgg
    15241 ttggaacgca gataaaaccg atgctggtag tgatcaaggc tgtattcgtg atctttgcgg
    15301 cgaggcagaa aaacaaacgg ctcggcatcg gccatttccg cgtccagcag gcgaacttca
    15361 ctggtggtgg cgctggccag atgaatgacc acataatgct tcgaggtcgt tttatgcagg
    15421 ctgacgtaat aggtatcgtc tttttcttcg taaatcagtt tatcttgcga tgctggcgta
    15481 ccgatggcgt gacgccagac ctgataaggc agcagcgtca ctggatgctt gcgaacatag
    15541 tagaaagtcc aggagtcatt tgcccagaca aagctgggtt caacgttatc cagcagttcc
    15601 gggtaccagt tacctgtttc cagattacga aaacgaatgc catactgccg tcgggaaaga
    15661 aaatcttctg ccagtgccat aatggtgtta tctggcgtaa tcgccattcc gcccatcgaa
    15721 taaaactcac tatgagccgc gcgcttgttg gcatcgagca atgtttccca ctcgtcccac
    15781 tcttcactga acgccgattg acgctggtag atagcatatt cacagcctgg ttcataaata
    15841 tgccgatagc ggtagccatt tttgatgtag ggcgcagaaa cttctcgttg cggaatgcgg
    15901 tcgatgattt cctttaagat gcgatcctgc aaggcttgtt gcgaggccat cacccgatga
    15961 ccgtaactat tttcttgttg cagatagtcc aggactttcg gctgagaacg cgtatcgtcc
    16021 cgcagccagt agtaattatc gatgcgcgta tcgccatgaa gcgtcatggc gtggggaatg
    16081 cgggcggctt ttggtagcat gttattgttc tttctggttg aaacatctta taagggtggc
    16141 aaaactcacc ggggatgcaa gcgaaacagg gtagtcattg cttagatgat gacaggtaat
    16201 ggcgcggata tcgaatgtta tgcagacaga gaaaatcagc ctgctcaaat ggctgtgcgg
    16261 ttctggataa cccgaaataa tcagcttcag gctatccaga gagcggaatt actccgccaa
    16321 agtacgtttt tgctgttcga gatcgcgttc aatgccttca cgaacatcct gggggatttt
    16381 cagcgcatca cccagtgcat tcaggtaact gcgttccata aaatggtcaa tatcaatagc
    16441 cgcgcagctc aggaaataga tttccagcgc ctcttcttca ttgcggaccc cggtagccag
    16501 gcgttgtgga tccagcggtt gttcgattgc ctgctcaatg agtacacgcc ccttctcttc
    16561 cacaccggct tctcgcaatt gctggtcgat tgccgcacgt tctttggcat caatatggcc
    16621 atcactttta gcggcaaaga ccagcgcaag gatcaaacgt tccgtacgct catccagcgg
    16681 cgtactttgc gcgccaaact gcggttcatc ctgatgcgcc gcgcgaattt tatctttgta
    16741 tttattccac agcaccgtac ccgctaccgc tccgccgcca accagtaacg cgtttgtgcc
    16801 atatttcgta agaagtttac gtgctgattt atttgcgacc agcagcccag ccagtccgcc
    16861 taatgcgcca ggcactaaaa gtttgcccaa tccctgatcc gcagacgagg aggtagaaga
    16921 acggctttgc ccaagcaggg attgcagttg atttaaccag ttagccatat ttgctcctca
    16981 ataaccattt ttagtgcctg tagcatagcg gatgaggatg tcagaaaatg tatattgaga
    17041 caaaagatgc gcaaataagc ggggcggtag gccgttattc aaagaaattc gcgccaggta
    17101 aggtgttcac tgtatataag gtgataaggg tattttaggt gaaagtagtt tgacgttttg
    17161 agtggaactg tttgatgctg attgcccggt tcgacgctgc tgcgtcttct caggactacg
    17221 attgtgttgc gttgtagggc ggacaaggca ctcgcgccgt atccgccaac ggtcaatcga
    17281 attatcagaa gccattaacg ccgctgatat tccgccattc ccgctttgca gtccacgctg
    17341 acctggtaat gaatatcggc acttttacca cgtacggtta gcggtactga ccatttatca
    17401 tctttcccct gaatgtcctg caaactgacc catgccacgg gatcgggctg accgacaatt
    17461 ttttgatcat cagcccagcg cgccacgcga ttttgttggt aatcacgttt tacgctcgcg
    17521 gcaattccgg cagcatccag accttcacac tttgggaaag tgaccgactt gctggtttca
    17581 ttattggcag cgaaaactga tgcgcaggca gaaaccaaca acagccctaa aaacgcccct
    17641 ctttttttca tgtttttctc catagcacaa tgattcagga gaaagcatgg tacaaattgt
    17701 caggagcgca agttgcttca ggccgcgtgt gaggcatctt cctcttcgga ttcagcaccg
    17761 acgacctgtg gggacggtaa tttgccggtt ttcagaatgg tgctcagcac atctttttgt
    17821 tctgccagcc atagcgaaag ggcttcacgt tgctcgtctt ccatttgcac tggagaattt
    17881 gtcagccagg tgtcaagcag atccgccgta tcaagcattt tgtcataagc atcggcttcc
    17941 tttttgctgg taaacgacat tttctcttcg ccctcacgaa tgactacgta tttaacttca
    18001 accgccattt gcagcctctc ataataactg tgattttata cagtatattt cttctcgatt
    18061 gggaaatcaa catcaacaat aaagacacgc gcaaacgttt tcgtttatac tgcgcgcgga
    18121 ataaatcagg ggatattcgt tatgacgtta ttaggcactg cgctgcgtcc ggcagcaact
    18181 cgcgtgatgt tattaggctc cggtgaactg ggtaaagaag tggcaatcga gtgtcagcgt
    18241 ctcggcgtag aggtgattgc cgtcgatcgc tatgccgacg caccagccat gcatgtcgcg
    18301 catcgctccc atgtcattaa tatgcttgat ggtgatgcat tacgccgtgt ggttgaactg
    18361 gaaaaaccac attatatcgt gccggagatc gaagctattg ccaccgatat gctgatccaa
    18421 cttgaagagg aaggactgaa tgttgtcccc tgcgctcgcg caacgaaatt aacgatgaat
    18481 cgcgagggta tccgtcgcct ggcagcagaa gagctgcagc tgcccacttc cacttatcgt
    18541 tttgccgata acgaaaacct tttccgcgag gcggttgctg aaatcggcta tccctgcatt
    18601 gttaaaccgg tgatgagttc ctccggcaag gggcagacgt ttattcgttc tgcagagcaa
    18661 cttgctcatg catgggagta cgctcagcaa ggcggtcgcg ccggagcggg ccgcgtaatt
    18721 gttgaaggcg tcgttaagtt tgacttcgaa attaccctgc taaccgtcag cgcggtggat
    18781 ggcgtccatt tctgtgcacc ggtaggtcat cgccaggaag atggcgacta tcgtgaatcc
    18841 tggcaaccac agcaaatgag ccagctggct ttggaacgtg cgcaagagat tgcccgtaaa
    18901 gtggtgctgg cactgggcgg ttatgggctg tttggtgtcg agctgtttgt ctgtggtgat
    18961 gaggtgattt tcagtgaggt ctcccctcgc ccacatgata ccgggatggt gacgttaatt
    19021 tctcaggatc tctcggagtt tgccctgcat gtgcgagcct ttctcggact tccggttggc
    19081 gggatccgtc agtatggtcc tgcagcttct gccgttatcc tgccacaact gaccagtcag
    19141 aatgtcacgt ttgataatgt gcagaatgcc gtaggcgcag gcatacagat tcgtttattt
    19201 ggtaagccgg aaattgatgg cagccgtcgt ctgggtgttg cgctggcgac cgctgagagt
    19261 gttgatgaag cgattgagcg cgcaaagcac gccgccggac aggtaaaagt acagggttaa
    19321 acccggcaaa aaaacgctac aaaaatgccc gatcctcgat cgggcatttt gacttttaca
    19381 gcttagcgcc ttctacagct tcacgcgcca gcttagtaat gcggtcgtaa tcgcccgctt
    19441 ccagcgcatc tgccggaacc agccaggaac caccgatgca cagcacgctt ttcagcgcca
    19501 ggtagtcacg gtagttagcc ggagaaatac cacctgtcgg gcagaaacgg acctgggaga
    19561 acggacccgc gatcgcctgc agggctttca caccgccgtt agcttcagcc gggaagaatt
    19621 tgaactcttt caaaccgtag tccataccca gcatcagttc ggaaacagtg ctgatccccg
    19681 gaatcagagg aatagtccct tcggtagcag ctttcagcag cggctcggtc agacccgggc
    19741 taattgcaaa ctgtgcaccc gcttcagtga cttctgccag ctgctgtgga ttcagcaccg
    19801 taccggcacc cacaatcgct tcaggcactt ctttggcgat agcacggata gcgtcaactg
    19861 cgcactcggt acgcagagtc acttccagaa cgcgcacccc accagcaacc aacgcttttg
    19921 ccatcggcac cgcgtgttcc agttttttta ccacgataac cggtacaacc gggccggtgg
    19981 tcaggattga ttctgcactt gttttccagt ttttcatcag agttttctct cgcctgatta
    20041 caaatttgtc gtcttaaaaa gtgatacagg ttgcgccctg ttcggcaccg gacagttttt
    20101 cacgcaaggc gctgaataat tcacgtcctg ttcccacgcg tgacgcgctc aggtcaggaa
    20161 tgtgtggttc gcgagcagcc agttccgctt cgtctaccag cagcgtcagt tcgcctgtct
    20221 gtccattcac acgaatgatg tccccgtcgc gcacttttgc cagcagcccg ccatcgtagg
    20281 cttctggtgt tacgtggata gctgacggca ctttacctga agcgccggag agtcgtccat
    20341 cagtaactaa cgcaattttg aaacaccggt ccaataatac accaagtggc ggcatgagtt
    20401 tatgtaattc tggcattccg ttcgcttttg gcccctgatg acggacaaca acgacacaat
    20461 cgcggtccag caaacccgct tcaaaggccg gcataacgtc atgctggctt tcaaaaacaa
    20521 ccgcaggcgc ttcaatcacc tggttctcta ccggcacggc agaggttttc ataaccgcac
    20581 ggcccaggtt accgcttaac acttttgtcc caccatgatg agagaaaggt tgttcgaagg
    20641 aagcgatcac attgctgtcg agtgattttt ccgccccttc ccgccagtcc agttcaccat
    20701 tattcagcca tggttcaagg gtataacgag acagaccaaa gcctgccacc gtattgacat
    20761 cttcatgcag caggcctgct ttgagcagtt cacgcaccag aaccggtacg ccacctgccg
    20821 cctggaagtg gttaatatcg gccggaccgt tcgggtagag acgtgccatc agcggtacaa
    20881 catcagaaag gtcagagaag tcatcccagt taatctgaat accggctgcg cgcgccatcg
    20941 ccaccaggtg catggtgtgg ttagtggaac caccggtcgc cagcagtgca acgataccgt
    21001 tcaccaccac tttctcatcg atcatcttac cgatcggcat ccattcatta ccattaccgg
    21061 tcatgcgcgt aacctggcgc gcagctgcag cggtcaaagc atcgcgcagc ggagaatccg
    21121 gatgaacaaa agaagagcct ggcaactgca tccccataaa ctccaccacc atctggttgg
    21181 tgttggcagt accgtagaaa gtacatgttc ccggcgcatg ataagacgcg gcttctgact
    21241 ccagtagtgc catgcggtcc actttacctt cggcataaag ctggcgaata cgcacttttt
    21301 ctttatttgg caaaccgctt gccatcggtc cagacggcac aaacaccgca ggcaaatgcc
    21361 caaacgacag ggctgccatc gtcagacccg ggacaatctt gtcgcacaca ccgaggaaca
    21421 gagcaccatc aaacatgtta tgggacagcc ccaccgccgc agacatcgca atcacttcgc
    21481 ggcttaacag cgacaattcc attccatcct gcccctgggt gacaccatca cacatcgccg
    21541 gaacaccgcc tgcaacctga ccaaccgcat ttgcttcatg cagagcttta cgaatgattt
    21601 ctggatagtg ttcataaggc tggtgcgcgg agagcatgtc gttataggag gtgatgatgg
    21661 cgatattgtt acgcaacatg cttttcaaag aggctttgtc ttctggctgg caggcagcga
    21721 aaccgtgtgc caggttaccg catgccaact gcgaacgatg aacggtcgaa gttttcgctt
    21781 gttctatccg ggcgaggtaa gcagagcgag tctcgcgcga acgttcaatg attcgatttg
    21841 ttacgcgtaa caattgtgga ttcataaagg ctcctgaaat tgagttgtca gagcaggatg
    21901 attcacaaca cgtttcattc agaggattca tgactgaaac gcctgtaacc ggagctcata
    21961 gggcaaaaac gtttcagtca gtgtaataaa aaaagcctcg tgggtgaatc cgcacgaggc
    22021 ctgaaagtgt aaaaattgtt ctacaatctg tgcaagatca tgttaccggt aaaataacca
    22081 taaaggatga gcgcagatat tactcaaact cattccagga acgaccatca cgggtaatca
    22141 tcgccactga ggcaacgggt ccccaggttc cggcctgata cggtttcggc gcatcattgt
    22201 ccatcgccca cgcctcagta atggagtcta cccatttcca ggcttcttcc acttcatcgc
    22261 gacgtacgaa cagtgcctga ataccacgca tggtttccag cagcagacgt tcataggcat
    22321 ctgccagatg cgtttgatta aaggtttctg aatagctcag atccagcttg gtgatttgca
    22381 ggttatgttt atggtcaagg ccaggaactt tatttagtac ctggatatcc acgccttcat
    22441 caggttgcag acggatagtc agtttattct gcggcagatc ctgccacgac tctttaaaca
    22501 gattcagttc aggtgttttg aaatagacca cgacttcaga acatttggtc ggcagacgtt
    22561 taccagtacg caggtagaat ggcacaccgg cccagcgcca gttatcaatg tcgacgcgga
    22621 tcgccacgaa ggtttctgta ttgctgctct tgttcgcgcc ctcttcttcc agatatcccg
    22681 gcactttttt gccctgggcg aagcccgcag tatattgccc gcgtacggtt ttttcgcgca
    22741 cgttggagcg gtcaatgcgg cgcagagact tcagtacttt cactttttca tcgcggatgc
    22801 tgtctgcact caggtcagac ggcggagaca tcgcaatcat gcaaagaatt tgcagcaggt
    22861 ggttctggat catgtcgcgc atctgaccgg ctttatcaaa atagccccag cgcccttcga
    22921 tccccacttc ttctgccacg gtaatctcaa catgatcaat ggtgcgattg tcccagttat
    22981 tcacaaacag ggagttagca aaacgcagcg ccaacaggtt cagcaccgtt tctttaccaa
    23041 gatagtggtc gatacggtaa acctggcact cctcgaagta ttcgccaacc tgatcgttga
    23101 tttcctgcga ggtcgccagc gacgtcccca gcggtttctc catgactacg cgtgccggtt
    23161 tagcattcag ttttgcctca ccaagccctt tgcaaattgc gccaaaagtg ctgggcggca
    23221 tggcaaagta gttaatggtg atacgatttt tttgatccag catcgcgccg agacggctga
    23281 atgcagcagt gtcattgaca tcgagattac aaaaatccag acgtgcactc agggtgtccc
    23341 ataaaccttc atcaatggtt tctttcatga aagtttcgag cgcctcgcgg acaactttgg
    23401 tataagccgc tttatcccag tcagcacgcc ctacgccgat aatccgggtg tccgggttga
    23461 gctgaccggc tttttccagt tgatacaggg aaggcagcaa tttacgacgc gcaaggtcgc
    23521 ctttcgcgcc gaaaatgacc aggtcacagg cctgggctgt ttgcgttacc gccatgtcat
    23581 tctccttaag ttaactatcc cggtacttaa gccagggtat acttgtaatt ttcttacggt
    23641 gcactgtact gcttttacga gcttgcgaaa actgtaaacg cttatccacc cgtgcgatta
    23701 cggtaaaagc gcgcaaagtg cggcaaaact gataaaaaaa tcatcgtttt ccattgggta
    23761 aaaatctgac actgatcatg ttatgaaaaa aaataacaac ttttttatct gcttttgtca
    23821 ttaacacagc acacaggcgt aatatctgac aaaactgcat ttcgatttct ttcagagcgg
    23881 aaatcgtcat tacccgtgag tctctttaca tcatgaatat gctggaaaaa atccagtctc
    23941 agctggaaca tttgagcaaa tctgagcgca aagttgccga ggtcattctg gcttcgcccg
    24001 ataacgcgat ccattcgagt attgctgctc tggcgctgga agccaatgtt agcgaaccga
    24061 cggtgaatcg tttctgtcgc agcatggaca ctcgcggttt tcctgatttt aaacttcatc
    24121 tggcacaaag tctggcgaat ggcactccct atgttaatcg caacgtcaat gaagatgaca
    24181 gcgttgaatc atacacgggg aaaatatttg agtccgcaat ggcaacgctc gatcatgtcc
    24241 gtcattcact ggataaatct gccatcaacc gcgccgtcga tttgctcact caggcaaaaa
    24301 aaatcgcctt tttcggatta ggctcttcag ccgccgttgc ccacgatgcg atgaataagt
    24361 tctttcgttt taatgttccg gtggtgtact ccgatgatat cgtgctgcaa cgcatgagtt
    24421 gtatgaattg tagcgacgga gacgtggtgg tgctgatttc tcacacggga agaacaaaaa
    24481 atctggtcga gctggcgcag ctggcacgcg aaaacgacgc catagtgatt gccctcacct
    24541 ctgcgggtac cccgctcgcc cgggaagcaa cgctggcaat taccctcgac gtaccggaag
    24601 atactgacat ttatatgccc atggtttctc gacttgcgca gctgaccgtg atagatgtgc
    24661 tggcgacagg atttactttg cgacgcggtg caaaattcag agataacttg aagcgggtca
    24721 aggaagcgct gaaggaatcg cgttttgata agcagctact taatttaagt gacgatcgct
    24781 aaaaacgact gtcactgtcc taatcttata cgacatccga atgagattaa tttatcgcca
    24841 tcgcggcgtt atttcattcg gatttcatgt tcaagcaaca cctggttgtt tcagtcaacg
    24901 gagtattaca tgtccagaag gcttcgcaga acaaaaatcg ttaccacgtt aggcccagca
    24961 acagatcgcg ataataacct tgaaaaagtt atcgcagcgg gtgccaacgt tgtacgtatg
    25021 aacttttctc acggctcgcc tgaagatcac aaaatgcgcg cggataaagt tcgtgaaatt
    25081 gccgcaaaac tggggcgtca tgtggctatt ctgggtgacc tccaggggcc caaaattcgt
    25141 gtatccacct ttaaagaagg caaagttttc ctcaatattg gggataaatt cctgctcgac
    25201 gccaacctgg gtaaaggtga aggcgacaaa gaaaaagtcg gtatcgacta caaaggcctg
    25261 cctgctgacg tcgtgcctgg tgacatcctg ctgctggacg atggtcgcgt tcagttaaaa
    25321 gtactggaag ttcagggcat gaaagtgttc accgaagtga ccgtcggtgg tcccctctcc
    25381 aacaataaag gtatcaacaa acttggcggc ggtttgtcgg ctgaagcgct gaccgaaaaa
    25441 gacaaagcag acattaagac tgcagcgtta attggcgtag attacctggc tgtctccttc
    25501 ccacgctgcg gcgaagatct gaactatgcc cgtcggctgg cacgcgatgc aggatgtgat
    25561 gcgaaaattg ttgccaaggt tgaacgtgcg gaagccgttt gcagccagga tgcaatggat
    25621 gacatcatcc tcgcctctga cgtggtaatg gttgctcgcg gcgacctcgg tgtggaaatt
    25681 ggcgatccgg aactggtcgg cattcagaaa gcgttgatcc gtcgtgcgcg tcagcttaac
    25741 cgcgcagtaa tcacggcgac ccagatgatg gagtcaatga ttactaaccc gatgccgacg
    25801 cgtgcagaag tcatggacgt agcaaacgcc gttctggatg gtactgacgc tgtgatgctt
    25861 tctgcagaaa ctgctgctgg tcagtatccg tcagaaaccg ttgcagccat ggcacgcgtt
    25921 tgcctgggcg cggaaaaaat cccgagcatc aacgtttcta aacaccgtct ggacgttcag
    25981 ttcgacaatg tggaagaagc cattgccatg tcagcaatgt acgcggctaa ccacctgaaa
    26041 ggcgttacgg cgatcatcac catgaccgaa tcgggtcgta ccgcgctgat gacctcccgt
    26101 atcagctctg gtctgccaat tttcgccatg tcacgccatg aacgtacgct gaacctgact
    26161 gctctctatc gcggcgttac gccggtgcac tttgatagcg ctaatgacgg cgtagcggct
    26221 gccagcgaag cggttaatct gctgcgcgat aaaggttact tgatgtctgg tgacctggtg
    26281 attgtcaccc agggcgacgt gatgagtacc gtgggttcta ctaataccac gcgtatttta
    26341 acggtagagt aagtacgttg ccggatgcgg cgaaaacgcc tcatccggcc tacagttcaa
    26401 tgataattca acagatttcg aatcttctga agcaaacttg aacttatcac cagtcgaagg
    26461 cctctcctcg cgagaggcat tttttatttg atgggataaa gatctttgcg cttatacggt
    26521 tggatttcgc ccggtttgcg agttttcagc aattttaata tccaggtgta ttgttctggt
    26581 cgcggaccaa caaaaatctc gacttcttca ttcatccgcc gcgcaatcgt atgatcatcc
    26641 gcctctaaca gatcatccat cggtgggcgc acctgaatcg tcaaacgatg cgttttgcca
    26701 tcataaatcg gaaacagcgg tacaacgcgc gcacggcaca ctttcatcaa acgaccaatc
    26761 gcaggcaacg tcgctttata ggtggcaaag aaatcaacaa attcgctgtg ctctgggcca
    26821 tgatcctgat cgggtaaata atatccccag tagccctgac gtaccgactg gatgaatggt
    26881 ttaataccat catttctcgc atgcagacga ccgccaaagc gacgacgcac cgtgttccag
    26941 acataatcaa aaaccgggtt gccctgatta tggaacatcg ctgccatttt ctgcccttgc
    27001 gaggccatca gcatggcagg aatatcgacg gcccaaccgt gcggcaccag aaaaatcact
    27061 ttctcgttat tacgccgcat ctcttcgata atctccagcc cttgccagtc aacgcgcggc
    27121 tgaattttct ccggcccgcg tattgccaac tcagccatca ttgccatcgc ttgcggcgcg
    27181 gtggcaaaca tctcatctac aatcgcttcg cgttcagctt cactacgttc tggaaagcag
    27241 agcgacagat tgattaacgc acgacggcgt gagctttttc ccagtcgtcc ggcaaaacgt
    27301 cccagccgtg ccagaatggg atcacggaac tttggcggcg ttaaagcgat acccgccatc
    27361 gcggctacgc ccagccatgc tccccagtag cgcgggtggc gaaaggattt atcaaactca
    27421 ggaatgtatt cgctattatt ttttttcgtt tccatgcttt tccagtttcg gataaggcaa
    27481 aaatcaatct ggtgatagtg tagcggcgca acttgccccg caccaaataa aaaaagccgg
    27541 tactgactgc gtaccggctg cgaatggatg ttaattaatc aaaccgtagc tgcggcacaa
    27601 tctctttggc ctgcgccagg aattcgcgac gatcggagcc ggtcagccct tcggtacgcg
    27661 gcagttttgc cgtcagcggg tttacggcct gctggtttat ccatacttca tagtgcagat
    27721 gcggcccggt tgaacgtccg gtattaccgg aaagcgcgat acggtcgcca cgtttcacct
    27781 tctgtcccgg tttcaccagg atcttgcgca agtgcatata acgcgtggtg tagctgcgac
    27841 catgacgaat agccacataa taacctgctg cgccactgcg tttggcaacc accacttcac
    27901 cgtcacccac tgaaagcact ggcgtacctt gtggcatggc aaaatcaaca cctctgtgtg
    27961 gcgcaacgcg accggtcacc ggattagtac gacgcgggtt aaagttagat gagatacgga
    28021 actgtttcgc cgtcgggaat cgcaagaatc ctttcgccag accagtaccg ttacgatcgt
    28081 agaatttgcc atcttcagcg cggattgcgt aataatcttt accttctgaa cgcaaacgta
    28141 cgcccagcag ctggctttgc tcacgtttac catcaagcat ttctcgtgac attaacaccg
    28201 caaattcatc gccttttttc agtttgcgga aatccatttg ccactgcatg gctttaatca
    28261 ctgcgctcac ttcggcgctg gttaaaccgg cgtttctggc gctggcaaca aagcttcccc
    28321 cgacggtacc tttcagcaga ttgttgaccc actctccttg ctgcatttcg ctggtcattt
    28381 taaaaccgtt agcggcagta cggtcatagg ttcgggtttc acgacgagac acttcccagg
    28441 tgaggcgctg cagttcgccg tccgcggtta atgtccagga gagttgttga ccgattttca
    28501 ggttacgcaa ttctttgtcg gcagcagcca gttgggtgat atcacccata tcaataccat
    28561 actgattgag aatgctgctt agcgtatcgc cagtggaaac aacatattca tgcacgcccg
    28621 cttcaccggc gattttgtca tccagttcat cctggggaat ggcttcatct tcttgtgcag
    28681 cttgatcaat cggctcactg gcttcaggta agagcgaacg aatttcgttc tgttccagct
    28741 caatggtttt gacaattggc gtggcatcgc ggtgataaac atagggccgc cagacagcga
    28801 cggccagagt aagaacggtg agcgacccta acataacgcg gtgtggtcgc ggtaaattat
    28861 taaacgccag ggcgacagag cgggctatct gttgcacgta atcacttcct cattaatctc
    28921 ctttcaggca gctcgcatac tggttggcta attgattcag gaattctgaa tagcttgttt
    28981 tacccagttt gatattcgtc cccaggggat ccaacgttcc catacgaacg gatgtccctc
    29041 gtgcgacgct ctcaacgacc gctggcctga actgtggctc agcaaaaacg caggttgctt
    29101 tttgctcaac caactgtgtt cttatttcat gtaaacgctg cgcgccaggt tgaatctcag
    29161 ggttaacggt aaaatgacca agcggtgtca gtccgaactg tttttcgaag tagccgtaag
    29221 catcgtgaaa aacgaaataa cctttcccct tgagcggcgc gagctcgtta ccaacctgcg
    29281 tttcggttga ggctaattgt gcctcaaaat ccttcaggtt ggcgtcaagt ttggctcgac
    29341 tttgcggcat aagttccact aattttccat ggattgcaac cgctgtagcc cgcgctatct
    29401 ctggggaaag ccaaagatgc atgttgaaat cgccgtgatg gtgatcttcg tcactttttt
    29461 ccgcgtggtc gtgatcatca tcatcgtcgt gaatactttt catcagcagc ggtttcacat
    29521 cttctagctg cgcaatcgtt acctgtttcg cttctggtaa tttacttacc ggtttttgca
    29581 tgaacgcttc catctccggg ccaacccaaa cgactaagtc cgcgttctgt aagcgtttta
    29641 catccgatgg acgcagtgaa taatcatgtt ctgaagcgcc gtcaggcagt aaaacctgcg
    29701 tttctgttac cccatcagca atggcagaag cgatgaaccc aacgggttta agcgaagcga
    29761 caacggcggc atctgcggcc tgtgttgcac cgccccagag agcggcggat aatgctgcga
    29821 aaagaagcgt ttttttatgt aacataatgc gaccaatcat cgtaatgaat atgagaactg
    29881 tgatattata acatttcatg actactgcaa gactaaaatt aacatgacaa gtctggtttc
    29941 cctggaaaat gtctcggttt cttttggcca acgccgcgtc ctctctgatg tgtcgctgga
    30001 acttaaacct ggaaaaattt tgactttact tgggccaaac ggcgcaggta agtcgacact
    30061 ggtacgggta gtgctcgggc tggtaacacc cgatgaaggg gttatcaagc gcaacggaaa
    30121 actgcgcatc ggctatgtac cgcagaagct gtatctcgac accacgttgc cactgaccgt
    30181 aaaccgtttt ttacgcttac gccctggcac acataaagaa gatattttgc ctgcactgaa
    30241 acgtgtccag gccgggcatc tgattaacgc accgatgcaa aagctctcgg gtggcgaaac
    30301 gcagcgtgta ctgttagcgc gagcattgtt aaatcgaccg caattattag tgctggatga
    30361 acccactcag ggcgtggatg tgaatggtca ggtggcgtta tatgacctta ttgaccaact
    30421 gcgtcacgaa ctggattgtg gcgttttaat ggtatctcac gatctgcatc tggtaatggc
    30481 aaaaaccgat gaagtgcttt gcctgaatca ccacatttgt tgttccggca caccggaagt
    30541 tgtttccctg catccggagt ttatttctat gtttggtcct cgtggtgctg aacaactggg
    30601 tatctatcgc catcatcata atcatcgtca cgatttacag ggacgaattg ttttgcgtcg
    30661 gggaaatgat cgctcatgat tgaattatta tttcccggtt ggttagccgg gatcatgctc
    30721 gcctgtgccg cgggtccgct gggttcgttt gtagtctggc gtcgtatgtc ttatttcggt
    30781 gatacgctgg ctcatgcctc attacttggc gtcgcgtttg gtttgttgct ggacgtgaat
    30841 ccattctatg cggtgattgc agttacgctg ctgctggcgg gcggtctggt atggctggag
    30901 aagcgtccac agctggcgat cgacacgtta ttagggatta tggcgcacag tgccctgtcg
    30961 ctgggcctgg tggtcgttag tctgatgtct aatattcgtg ttgatttgat ggcttacctg
    31021 ttcggtgatt tactggcagt gacgccagaa gatctcatct ctattgcgat tggcgtggtc
    31081 atcgtggtgg ctattatgtt ctggcaatgg cgcaatttgc tgtcgatgac gattagcccg
    31141 gatctggcgt ttgttgatgg tgtgaaatta cagcgcgtga aattgttgtt gatgctggtg
    31201 acggcattga cgattggtgt agcgatgaaa tttgtcggcg cgttgattat tacttcactg
    31261 ctgattattc ctgctgctac tgcacgtcgc tttgcccgca cgccggaaca gatggctggt
    31321 gtcgctgttt tggtggggat ggtggcagtg actggcggtt taaccttttc cgcattttac
    31381 gatacacctg caggcccgtc ggtggtgcta tgtgcggcac tgttatttat tatcagtatg
    31441 atgaaaaagc aggccagcta atctatcgct gaacacattt gtcggatgcg gcgcgagcgc
    31501 cttatccgac ctatggttcg ctatctctgg tatatgcctg ataagacgcg aatagcggcg
    31561 catcaggcaa cactgccagt gtcggatgcg ccgcgagcga ccgatccgac ttacggcatt
    31621 tctggcggcg tgatgccgaa gtggttccac gcccgcactg tcgccatacg cccgcgcggt
    31681 gtacgctgca aaaagccttg ctgaatcaaa taaggttcca gcacatcctc aatggtttca
    31741 cgttcttcgc caatggcagc cgccaggtta tccagaccta ccggcccacc aaagaactta
    31801 tcgattaccg ccagcaacaa tttgcggtcc atataatcga aaccttcggc atcgacattc
    31861 agcatatcca gcgcctgagc agcgatatca gccgagatgg tgccatcgtg cttcacttcg
    31921 gcgaaatcac gcactcgacg cagcagacgg ttagcaatgc gcggcgtacc gcgagcgcga
    31981 cgagcaactt ccagcgcgcc gtcatcactc atctcaagcc ccataaagcg tgcgctgcga
    32041 ctgacgatat attgcagatc cggcacctga taaaactcca gacgctgcac aataccaaaa
    32101 cgatcgcgca acggtgatgt cagcgaacct gcgcgcgtgg ttgcgccaat cagtgtaaac
    32161 ggcggcaaat caattttaat ggagcgtgcc gccggacctt caccaatcat gatatccagt
    32221 tggtagtctt ccattgccgg atacaacacc tcttccacca ctggtgaaag acggtggatc
    32281 tcatcaataa acagtacatc gtgtggttca aggttagtga gcattgctgc cagatcgccc
    32341 gccttttcca gcaccggacc agaagtcgtg cgtaaattaa cgcccatttc attggcgaca
    32401 atattggcaa gcgtagtttt acccaacccc ggaggaccaa aaatcaacag atgatcgagg
    32461 gcatcgccgc gcagtttcgc tgctttgatg aaaatctcca tctgtgaacg aacctgcggt
    32521 tgaccaacat actcttccag taatttaggg cgaatggcgc gatctgccac atcttccggc
    32581 aaagtggtac cggcagaaat cagacggtct gcttcaatca tcctttacct cataacgcgg
    32641 cgcgtagggc ttcgcgaatt aatgtttcac tgctggcgtc agggcgagcg attttgctca
    32701 ccatacggct tgcttcttgt ggtttatagc ccagcgccac cagcgcagca accgcttcct
    32761 gttcagcatc gtcggtcgcc gggctggcag gtgacgtgag taccaggtcg gcggctggcg
    32821 taaagagatc gccatgcaaa cctttaaatc ggtctttcat ttcgacaatc aagcgttcgg
    32881 cggttttttt gccaataccc ggcagtttca ccagtgcccc cacttcttca cgctcaacgg
    32941 cattaacgaa ctgctgcgct gacattccgg agaggatcgc cagcgccaac ttcgggccga
    33001 cgccgttggt tttgatcaac tctttgaaca acgtgcgctc ttgtttattg ttaaaaccgt
    33061 acagcagttg cgcgtcttca cgcaccacaa agtgggtgaa aacgatcgct tcctgacccg
    33121 cttcagggag ttcataaaaa caggtcatcg gcatatgcac ttcatagcct acgccgccca
    33181 cttcaattaa caccagcggg ggttgttttt caatgatgat gcctctgagt ctgcctatca
    33241 catgacgctc ctgcgtaatg aatcaaagat aatgctgtat gataaaaaaa tgctggatag
    33301 atatccagcg aaggatgaag aaaacttgcg aggtgtcccg atgatctgaa aaatggcgca
    33361 gtataattta ttctacagat tatattggaa gcaaatattt aaatattaca tattcagcga
    33421 agaaatgtgt aataaaaata cacatcgcga cccctgaaaa aataaatttt ttatgctatt
    33481 acgtatattc atatctattt caatggaatg acaacgtgaa tgttaattat cctgctgaat
    33541 atgaaattgg tgatatcgtc tttacatgta taggtgctac cttatttggt caaatatcag
    33601 ctgcatcaaa ttgctggagt aatcacgtcg ggatcattat cggtcataac ggtgaagact
    33661 tcctggttgc agaaagccgg gttccccttt caaccatcac cacgctatct cgttttatta
    33721 aacgttcgtc taatcaacgc tatgctataa agcgattaga cgctgggctg acagaacaac
    33781 aaaaacaacg aattgttgaa caggttccct cccggctacg caaactttac cacaccggtt
    33841 ttaaatacga atcttcgcgc cagttctgtt caaaatttgt ttttgatatt tataaagaag
    33901 cgctatgtat tccggtgggt gaaatagaga cgtttggaga attgttaaat agcaatccga
    33961 atgcaaaact cactttctgg aaattctggt tcttaggttc tattccgtgg gagcgtaaaa
    34021 ccgtcacgcc agccagtttg tggcatcatc cgggtttggt gttgattcat gcggagggag
    34081 tggaaacgcc tcagccggaa ctgaccgagg cggtataact taacgcagtc gccctctcgc
    34141 caggttcagc cgcgattcgc tcatctgcat cgcattctga ctgacgtgac aatgggtaat
    34201 cgcgatagct aatgcatcgg cagcatccgc ctgtggatta gcgggcagtt tcagcaaggt
    34261 gcggaccata tgctgcacct ggcttttttc ggcactacca atacctacca ccgtttgctt
    34321 tacctgacgt gccgcatatt caaataccgg caattcctga ttcaccgctg ccacaatcgc
    34381 cacgccgcgc gcctgaccca gtttcagggc tgagtcagcg ttcttcgcca taaagacttg
    34441 ttcaatggcg aaataatcag gctggaactg ggtgatgatt tccgtcacgc ccgcatagat
    34501 gagcttcaga cgagacggta aatcatccac tttggtgcgt atgcatccgc tacccaggta
    34561 ggacagttgc ctgcctacct ggcggatgac gccatagccg gtcacgcgcg aacccgggtc
    34621 aatgccgaga ataatagcca tcacgcgtct ccgttttgct gtttagcagg cctcatcaga
    34681 gagtcgctgc aacctcatca gagatttcac cgttatggta aacttcctgc acgtcgtcgc
    34741 aatcttccag catatcgatc agacgcatca gtttcggtgc ggtttctgca tccatatcag
    34801 ctttggtgga cgggatcatg gaaacttccg cgctgtctgc tttcagacct gccgcttcca
    34861 gagcgtcgcg tactttgccc atttcttccc atgcagtgta gacatcaatc gcgccgtcat
    34921 cataggtcac aacgtcttca gcaccggctt ccagggctgc ttccatgatg gtgtcttcat
    34981 cgcctttctc gaaggagatc acgccttttt tgctgaacag ataagctacg gaaccatcag
    35041 taccgaggtt accgccacat ttactgaatg catggcgcac ttcagcaacg gtacggttgc
    35101 ggttgtcaga cagacattca atcatgattg ccgtgccgcc aggaccgtaa ccttcgtaga
    35161 tgatggtttc catgtttgca tcatcatcac cgcccacacc tcgtgcaatt gcgcggttca
    35221 gagtgtcacg ggtcatgttg ttagacagtg ctttatcaat tgctgcacgc agacgcgggt
    35281 tagcgtccgg atcaccaccg cccagcttag ccgcggttac cagctcacga atgattttag
    35341 tgaagatttt accgcgctta gcatcctgcg cagctttacg atgtctggtg ttggcccatt
    35401 tactatgacc tgccataaaa atatctccag atagccctgc ctgttcaggc agcgttaatt
    35461 acaaactgtt caatcgcctg ccggttgctc caggacttag tgagcgccgc agcagcaggc
    35521 gcgtcaagcc acttgtaagc cagatgttca gtgaaaacga tctggcgctc gtgcgggagc
    35581 gcaagacaga accatgattc cgtattacgc gtcacgcccg gcgcatagcg atgacgtaaa
    35641 tgtgaaaaaa tttcaaactc taccgtgcgc tgacagtcaa ttaaggtcag ttgttcagcg
    35701 acaacatcaa tggtgacctc ttcctttact tcgcgcatgg cagcttgcgg cgcggtttca
    35761 ccctcttcca cgctgccggt taccgactgc cagaaatcgg gatcgtcacg ccgctgcaac
    35821 atcagcaccc gtttcgtatc ttgtgcatag atgaccacta agatcgaaac gggacgctta
    35881 tacactttat ccttcacgct gcctcttcgt tgactgcctt cgctcatccc attcacatag
    35941 ttatctatgc tcatgggagt tcactcagtt gccgcctcga tgcaacgcga atgatttcgt
    36001 gtatttgagt catatcagtt attctcgtcc ttcttcacaa cctgaatgct cagctcagcc
    36061 agtgcagtcg ggttagcaaa gctcggtgct tcagtcatca gacacgctgc cgccgtggtt
    36121 ttcgggaagg cgataacgtc acggatattg tcggtgccgg tcagcagcat ggtcagacgg
    36181 tcaagaccga atgccagacc tgcgtgcggc ggagtaccgt atttcagggc gtcgagcagg
    36241 aagccgaatt tctcgcgctg ttcctcttcg ttgataccca gaataccaaa caccgtctgc
    36301 tgcatatcac cattatggat acgcacagaa ccaccgccca cttcgtaacc attgatgacc
    36361 atatcgtaag cgttcgccac cgcattttct ggtgcagctt tcagttctgc agcagtcatg
    36421 tctttcggtg aggtgaacgg atggtgcatt gccgtcaggc cgccttcacc gtcgtcttca
    36481 aacatcggga agtcgataac ccacagcggt gcccatttgc tttcgtcggt cagaccaagg
    36541 tctttaccca ctttcaggcg cagtgcgccc atcgcgtcgg caacaatttt cttgttgtcg
    36601 gcaccgaaga aaatcatatc gccatcttgc gcggcagtac gatccaggat ggcttcgatg
    36661 atttctgcat taaggaactt cgctaccggg ctgttgatac cttccagacc tttcgcgcgt
    36721 tcgttaactt tgatgtaagc cagacctttc gcgccgtaga ttttaacgaa gttaccgtat
    36781 tcgtcgatct gcttacgggt caacgatgcg ccgcccggaa cacgcagagc ggcaacacgg
    36841 cctttcggat cgttcgccgg acctgcaaat actgcaaact caacagattt cagcagatcg
    36901 gcaacgtcgg tcagttccat cgggttacgc agatccggtt tatcagaacc ataacggcgt
    36961 tctgcttctg caaaggtcat taccgggaaa tcgcccagat ccacgccctt cacttccagc
    37021 cacagatgac gcaccagcgc ttccatcact tcacgcactt gcggcgcggt catgaaagaa
    37081 gtttccacat cgatctgagt aaattcaggc tgacggtcag cacgcaggtc ttcgtcacgg
    37141 aagcatttaa cgatctgata gtagcggtca aagccggaca tcatcagtag ctgtttgaac
    37201 aactgcgggg attgcggcag cgcgtagaat ttacctttgt gcacacgaga aggcaccagg
    37261 tagtcacgcg cgccttccgg cgtggctttg gtcagcatcg gagtttcgat gtcgaggaag
    37321 ccgtggtcat ccataaaacg gcgcaccagg ctggtgattt tagcgcgggt tttcaggcgc
    37381 tgagccattt ccgggcgacg caggtcgagg tagcggtatt tcagacgcgc ttcttcggtg
    37441 ttgacgtggt tagagtcaag cggcagaaca tctgcacgat tgatgatagt cagcgaggac
    37501 gccagtactt cgatttcgcc agtcgccata tcgcggttaa tatttttttc gtcacgcgca
    37561 cgtacggtgc ccgtgacctg aatgcagaac tcattacgca gttcagaggc cagctttaac
    37621 gcgtccgcac gatccggatc gaaaaatacc tgcacgatac cttcgcggtc gcgcatatcg
    37681 atgaagatca ggctaccaag atcacgacga cggttgaccc aaccacacag agtcacctgc
    37741 tgccccacgt gggacaaacg gagctgtcca caatattctg tacgcatgag atatccctta
    37801 acttagctgc cggcggatgc cccctgctgc gcaggtgacc aagtcgcagc gttagctgta
    37861 tgtcacaact gaatgaaaaa aggcggctat tatactggaa attctgccgc accgtaagag
    37921 cctggcccgc gctggaacgc ctcgttacca ctttatatcg ggcctgaaat cagactctac
    37981 gccagtttgc tataaaggtg ttgcccgaac tcataaaaat taacaaaatt tgtcgttccg
    38041 ccatcggcta atcgcattaa ggtgagaggc acgattttgt tttgtcagga gtcatcatgc
    38101 ttgaacttaa tgctaaaacc accgcgctgg tggtgattga tttacaagaa ggcatcttac
    38161 cttttgccgg aggtccacat actgccgatg aggtggttaa tcgcgccggg aagctggcgg
    38221 cgaaatttcg cgccagcggt cagcccgtgt ttctggtgcg cgttggctgg tctgccgatt
    38281 acgccgaagc attaaaacag ccggttgatg ccccctcccc cgcaaaagtg ttgcccgaaa
    38341 actggtggca acatcctgct gcattaggtg caaccgacag cgatatcgaa atcatcaaac
    38401 gtcaatgggg tgcgttttac ggtacggatc tggagttgca attacgccgc cggggtatcg
    38461 atacaatagt gttatgtggg atctcgacca atatcggtgt tgaatccacc gcccgcaatg
    38521 cctgggaact cggttttaat ctggtgattg ccgaagatgc ctgtagcgcc gccagcgccg
    38581 agcagcacaa taacagcatt aatcatatct acccgcgcat cgcccgtgtg cgcagtgtgg
    38641 aagagatcct ccacgcgtta tgatttacat cggtctgccg caatggtcgc atcctaaatg
    38701 ggtgcggttg gggatcacca accttgaaga gtatgcccgc cactttaact gcgtgacgca
    38761 acatttttaa aaatcgacga agatcgtcca ggagcgccgt ttttcaaggg ttggatagat
    38821 gattcgttta ccatgtcgac gaacttcgac gaatttcgcc gataaaaagt gtcccataca
    38881 tgccccattg atgccccata aacaacatca ggaattgaca ctggttatcc atacagataa
    38941 aaataacacc tgtacataac cacagttaca ggagggtgtt atgcgtgttg agatctgtat
    39001 cgctaaagaa aaaatcagca aaatgccaaa aggttctgtt cctgccctgc aacaggaaat
    39061 gctacgacgt gtcggtaagc gttatgacga tgtagatgtt atcgttaaat ctgccagcaa
    39121 tgatggactc actattttaa gagccgtgga taaagaatcc gcgcaagagt ttgtacaaga
    39181 aacgcttcag gaagtctggg aaacagcgga tgactggttt attcactaat taatagattt
    39241 gcaggtgatt tccgttacag gcttatcacc tgtaaaacct tactagccgc ttatctgtaa
    39301 ctatggaaaa accgtaagtc tggctttaag gttaacaaaa ggatgtttat gccgattttt
    39361 cataactgac tgttttatat cacaagtgga gatgacttta tctacatcaa cctcctttat
    39421 gcaaaggata caggcgcaaa aggtaatcat caggtcagac cttatattcc ctcaaaatct
    39481 ttgaaacgct gattccatct attaatcata ctcgcactct gaacccttca gtcttcctta
    39541 ctgaacatgt atcgtcacat ggttgacccg atacagaatc acacgcaatt tattacaaca
    39601 accattatgt tggtaagacc tgaaacaaaa aagaatcagg agcaggggaa aagcagccca
    39661 ttgcaggata ctgaggtgcg actcattatc cagttcacca ggctctaagt gagcactaca
    39721 cactacagaa ctgacaggag cgtttctggc aatcctagtt caccaactgg tgatttatgt
    39781 tgatttctcg tcagaaacgc ttcatggaca catcaaactc cctaaaacca gtgaacaagc
    39841 aaataaattt tcataattgc tcacaacctt tctacaaaga caacatcaat atttttatag
    39901 tttatgacta taatcac
//
LOCUS       DAZGQL010000040        39769 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.040, whole genome shotgun sequence.
ACCESSION   DAZGQL010000040 DAZGQL010000000
VERSION     DAZGQL010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39769
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.040"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(275..820)
                     /gene="hemG"
                     /locus_tag="ACHKIR_002342"
     CDS             complement(275..820)
                     /gene="hemG"
                     /locus_tag="ACHKIR_002342"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HFO9990075.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQADVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMSSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
     gene            complement(832..2283)
                     /gene="trkH"
                     /locus_tag="ACHKIR_002343"
     CDS             complement(832..2283)
                     /gene="trkH"
                     /locus_tag="ACHKIR_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HFO9990076.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            complement(2322..2936)
                     /locus_tag="ACHKIR_002344"
     CDS             complement(2322..2936)
                     /locus_tag="ACHKIR_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HFO9990077.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITLLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            complement(2936..4267)
                     /gene="pepQ"
                     /locus_tag="ACHKIR_002345"
     CDS             complement(2936..4267)
                     /gene="pepQ"
                     /locus_tag="ACHKIR_002345"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HFO9990078.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPNSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENITRDLKLA"
     gene            4457..6646
                     /gene="fadB"
                     /locus_tag="ACHKIR_002346"
     CDS             4457..6646
                     /gene="fadB"
                     /locus_tag="ACHKIR_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0036125 - fatty acid beta-oxidation
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004165 - delta(3)-delta(2)-enoyl-CoA
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HFO9990079.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLVEGAMAILRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYSGFDR
                     VDVVVEAVVENPKVKKAVLAETEQKVRPDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFNANRFGQKNGLGFWRYKEDSKGKPKKEEDAVVDDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKMA"
     gene            6656..7819
                     /gene="fadA"
                     /locus_tag="ACHKIR_002347"
     CDS             6656..7819
                     /gene="fadA"
                     /locus_tag="ACHKIR_002347"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HFO9990080.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRHVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMIGDAQTCLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSGAFKNEIIPTGGHDADGVLTQFNYDEVIRPETTVEA
                     LATLRPAFDPVSGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSVSDIGVFEMNEAFAAQILPCIKDLGLIEQIDEKINL
                     NGGAIALGHPLGCSGARISSTLLNLMERKDAQFGLATMCIGLGQGIATVFERI"
     gene            complement(8011..8712)
                     /gene="fre"
                     /locus_tag="ACHKIR_002348"
     CDS             complement(8011..8712)
                     /gene="fre"
                     /locus_tag="ACHKIR_002348"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HFO9990081.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(8758..10251)
                     /gene="ubiD"
                     /locus_tag="ACHKIR_002349"
     CDS             complement(8758..10251)
                     /gene="ubiD"
                     /locus_tag="ACHKIR_002349"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HFO9990082.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            10418..10906
                     /gene="rfaH"
                     /locus_tag="ACHKIR_002350"
     CDS             10418..10906
                     /gene="rfaH"
                     /locus_tag="ACHKIR_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HFO9990083.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPSTPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            complement(10903..11685)
                     /gene="tatD"
                     /locus_tag="ACHKIR_002351"
     CDS             complement(10903..11685)
                     /gene="tatD"
                     /locus_tag="ACHKIR_002351"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HFO9990084.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKVPGAVLHCFTGTREE
                     MQACVAHGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(11727..12503)
                     /gene="tatC"
                     /locus_tag="ACHKIR_002352"
     CDS             complement(11727..12503)
                     /gene="tatC"
                     /locus_tag="ACHKIR_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HFO9990085.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            complement(12506..13021)
                     /gene="tatB"
                     /locus_tag="ACHKIR_002353"
     CDS             complement(12506..13021)
                     /gene="tatB"
                     /locus_tag="ACHKIR_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HFO9990086.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            complement(13025..13294)
                     /gene="tatA"
                     /locus_tag="ACHKIR_002354"
     CDS             complement(13025..13294)
                     /gene="tatA"
                     /locus_tag="ACHKIR_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HFO9990087.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            complement(13373..15013)
                     /gene="ubiB"
                     /locus_tag="ACHKIR_002355"
     CDS             complement(13373..15013)
                     /gene="ubiB"
                     /locus_tag="ACHKIR_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HFO9990088.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKPLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVATLEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            complement(15010..15615)
                     /gene="ubiJ"
                     /locus_tag="ACHKIR_002356"
     CDS             complement(15010..15615)
                     /gene="ubiJ"
                     /locus_tag="ACHKIR_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HFO9990089.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLAALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKALRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            complement(15629..16384)
                     /gene="ubiE"
                     /locus_tag="ACHKIR_002357"
     CDS             complement(15629..16384)
                     /gene="ubiE"
                     /locus_tag="ACHKIR_002357"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HFO9990090.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKTMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            complement(16479..17906)
                     /gene="rmuC"
                     /locus_tag="ACHKIR_002358"
     CDS             complement(16479..17906)
                     /gene="rmuC"
                     /locus_tag="ACHKIR_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HFO9990091.1"
                     /translation="MDFSIIVYAIIALVSVVIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFVDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            18123..18890
                     /locus_tag="ACHKIR_002359"
     CDS             18123..18890
                     /locus_tag="ACHKIR_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFO9990092.1"
                     /translation="MAETSSTTAGAQTLLRGLAVLNAVYNGCHDLKSIGEFTGTTRST
                     THRLVTVLVEQRYLRHVPTQGYQLGAKLIEFGARALESTSLYEIAQPVLQRLARYTLD
                     TVHLGIVEGDEVLYLEKINSQRGLEMRSRPGHRMPLAITGIGKALILNRTEEEWRTLF
                     KTCGDETKLGAFIQNMRRYAASGFAFDLEENEPTIRCVAAPVYNARDEIVAAISVAST
                     TTYMSLARLEELAPYVKSCAEEISAELGWGKHVRKDK"
     gene            18914..19918
                     /locus_tag="ACHKIR_002360"
     CDS             18914..19918
                     /locus_tag="ACHKIR_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HFO9990093.1"
                     /translation="MPGAPSFIALDWGTSSLRAWRFGESPNPQEKREFPWGIMKLPSQ
                     AATREDAFHDTFLRVCGDWLAQTPCPVLACGMLGSAQGWQSAAYLPCPVTLEGLAKQL
                     TPVIHQQQTMLHIIPGVIKEGEMPEVMRGEETQIFGAISMEPALQNAIHQGMPVLIGL
                     PGTHAKWAVVENNTITDFRTFMTGELFDVLSRHSILGATMHPGDEPHWDAFTHGLTAA
                     QEHHQTGLLSTLFSTRSRLLTSNLTSSSQGDYLSGLLIGHELCGLASSLLRDLPATTP
                     IALIGSANLNSRYSQAFSHVFPDRQIHAIPNATEHGLWRIAHAAGLLSTNARECTHAI
                     "
     gene            19908..20537
                     /locus_tag="ACHKIR_002361"
     CDS             19908..20537
                     /locus_tag="ACHKIR_002361"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001116470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008674 -
                     2-dehydro-3-deoxy-6-phosphogalactonate aldolase activity
                     [Evidence IEA];
                     GO_process: GO:0034194 - D-galactonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HFO9990094.1"
                     /translation="MPFNTLLQKTGLVAILRGVKPDEIVAIGEKLYAAGFRLIEIPMN
                     SPEALQSISLLRDALPKDCLVGAGTVLSVEQVVAVKEAGGQIIVMPHCDTAVIRRARA
                     LGMYCAPGVATPTEAFAAIEHGANAIKLFPAEQITPEVTKAWRAVIPQSVPMLPVGGI
                     TPETMARYLSHGANGFGLGSALYRPGMTPEQVYENAVLFMNAWNNLNSN"
     gene            20562..21536
                     /locus_tag="ACHKIR_002362"
     CDS             20562..21536
                     /locus_tag="ACHKIR_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HFO9990095.1"
                     /translation="MIKHLTLPKKMAAMVLLAGAALSIAPVQAASYPTKQIELVVPYA
                     AGGGTDLVARAFADAAKNHLPVSIGVINKPGGGGAIGLSEIAAARPNGYKIGLGTVEL
                     TTLPSLGMVRFKTSDFKPIARLNADPAAITVRADAPWNSYEEFMAYSKANPGKVRIGN
                     SGTGAIWHLAVAALEDKTGTKFSHVPYDGAAPAITGLLGGHIEAVSVSPGEVINHVNG
                     GKLKTLVVMADERMKTMPDVPTLKEKGVDLSIGTWRGLIVSQKTPQDVVDVLAKAAKE
                     TAEEPAFQDALQKLNLNYAWLDAASFQTQISEQEKYFDELLTRLGLKK"
     gene            21553..22059
                     /locus_tag="ACHKIR_002363"
     CDS             21553..22059
                     /locus_tag="ACHKIR_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001596577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HFO9990096.1"
                     /translation="MNTKQSVAQLAVPHRKRLSSTMVVALLLCVVAGAVMINAADFPA
                     TAIETDPGASAFPTFYACALIVLAVLLVIRDLLQAKPASCANAQEKPAFRKTATGIAA
                     TAFYIVAMSYCGYLITTPVFLIVIMTLMGYRRWVLTPGIALLLTAILWLLFVEALQVP
                     LPVGTFFE"
     gene            22072..23562
                     /locus_tag="ACHKIR_002364"
     CDS             22072..23562
                     /locus_tag="ACHKIR_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HFO9990097.1"
                     /translation="MFESELLTQGFSTLLNNPQALLFATFGVMLGIVIGALPGLTATM
                     GVAILLPFTYGMEPVSGLLMICGVFFGGVYGGSITAILLKIPGTPAAAATAIDGYELT
                     KQGKAGLALSAATFSSFSGGTLSIIVLMFLSPVLASWALKFSASESFALATFGLSIIA
                     SISGESLIKGLIAGVGGLLIATIGLDPMGGFPRFTGGFVELMNVPFIPVMIGLFAASE
                     AFRSMEQNQQIRQGAKVAIGSLLLPWQTLRRIALTILRSSGLGVFIGMIPGAGADIAA
                     FVAYNETRRFSKTPENFGKGEIKAVASCEAGANGCTGGALLPMLTLGIPGDAVTAIML
                     GALTLQGMQPGPLMFTDHGDMVYTLFVGMIFCYFMLLVLGLLSLKVIGNVVKIPGNIL
                     TPMILALCVVGTYALNNSLFDVGIMLIAGVVGYFMQKGGYPASPVVLALIMGPMAESN
                     FRRALSLSGGSLDFLYTRPITLALLTLAAFTLLTPIIRKIMRLRRQ"
     gene            complement(23596..24360)
                     /gene="udp"
                     /locus_tag="ACHKIR_002365"
     CDS             complement(23596..24360)
                     /gene="udp"
                     /locus_tag="ACHKIR_002365"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001593007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HFO9990098.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLLK"
     gene            24622..25434
                     /locus_tag="ACHKIR_002366"
     CDS             24622..25434
                     /locus_tag="ACHKIR_002366"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HFO9990099.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKRN"
     gene            25826..26959
                     /gene="tssD"
                     /locus_tag="ACHKIR_002367"
     CDS             25826..26959
                     /gene="tssD"
                     /locus_tag="ACHKIR_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein TssD"
                     /protein_id="HFO9990100.1"
                     /translation="MSDIIYLTITGEQQGCISSRCGTSASIGNRWQIGHEDEIFAFSL
                     SNSITNTGKGSQLHGLSFCKLIDKSSPLLINAINNNEQLFMEFDFYRINRFGRWEKYY
                     NIQLRGALLSAINHLFTENNLDTETITVSYEYILSRHLIANTEFSHLAFPDNYNRLFI
                     PRQKTKDDNGLKTLNSKAVGRLLAAGGIYNGNIEGFRETANKLGGDAPAGYDQVMDNK
                     GLLIAGASIVAGVVLGRMRFPDLESFEHFGARGAVSGRPFKPDLAGGPIENLTIDGVT
                     INREGIAIVEKHISRFDHDPANDVMISRLKKIANKELLPEKYDLNYYTHECREYQRYC
                     NLGWETGEPNGLDGYELWNNAHTATLEDFKIKDTDLFHPDAKK"
     gene            26956..27213
                     /locus_tag="ACHKIR_002368"
     CDS             26956..27213
                     /locus_tag="ACHKIR_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFO9990101.1"
                     /translation="MTITIVEFNVLKVMAEKDIDWSWMVLDRTLAIRNIPGFGNVANI
                     VTKLVNHGLVDIVNGEGNSKPRYRVSQYGLNLIKEQQDNLF"
     gene            27288..27533
                     /locus_tag="ACHKIR_002369"
     CDS             27288..27533
                     /locus_tag="ACHKIR_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFO9990102.1"
                     /translation="MITKNELNVLNVMTEKDINWNWMNLDRTLSIRQIPGFGNVVNIV
                     NKLANEGLVIIEDSGHKSMPHYHVSEKGYNLVQRENK"
     gene            complement(27702..29963)
                     /gene="metE"
                     /locus_tag="ACHKIR_002370"
     CDS             complement(27702..29963)
                     /gene="metE"
                     /locus_tag="ACHKIR_002370"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFO9990103.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLAVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHKVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            30081..31034
                     /gene="metR"
                     /locus_tag="ACHKIR_002371"
     CDS             30081..31034
                     /gene="metR"
                     /locus_tag="ACHKIR_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HFO9990104.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     ITEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            complement(30922..31821)
                     /gene="bioP"
                     /locus_tag="ACHKIR_002372"
     CDS             complement(30922..31821)
                     /gene="bioP"
                     /locus_tag="ACHKIR_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HFO9990105.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALNE"
     gene            complement(31897..32697)
                     /gene="yigL"
                     /locus_tag="ACHKIR_002373"
     CDS             complement(31897..32697)
                     /gene="yigL"
                     /locus_tag="ACHKIR_002373"
                     /EC_number="3.1.3.23"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HFO9990106.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNAEDAVPHYLRKLYLS"
     gene            complement(32705..33727)
                     /gene="pldB"
                     /locus_tag="ACHKIR_002374"
     CDS             complement(32705..33727)
                     /gene="pldB"
                     /locus_tag="ACHKIR_002374"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HFO9990107.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVIVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            33838..34458
                     /gene="rhtB"
                     /locus_tag="ACHKIR_002375"
     CDS             33838..34458
                     /gene="rhtB"
                     /locus_tag="ACHKIR_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HFO9990108.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKPL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            complement(34520..35140)
                     /gene="rhtC"
                     /locus_tag="ACHKIR_002376"
     CDS             complement(34520..35140)
                     /gene="rhtC"
                     /locus_tag="ACHKIR_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HFO9990109.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRSALKKEVVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTERWGIFALIIIET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(35204..37033)
                     /gene="recQ"
                     /locus_tag="ACHKIR_002377"
     CDS             complement(35204..37033)
                     /gene="recQ"
                     /locus_tag="ACHKIR_002377"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFO9990110.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENDVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPISASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            complement(37165..38034)
                     /gene="pldA"
                     /locus_tag="ACHKIR_002378"
     CDS             complement(37165..38034)
                     /gene="pldA"
                     /locus_tag="ACHKIR_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HFO9990111.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITRHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            38199..38666
                     /gene="yigI"
                     /locus_tag="ACHKIR_002379"
     CDS             38199..38666
                     /gene="yigI"
                     /locus_tag="ACHKIR_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase YigI"
                     /protein_id="HFO9990112.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            38718..39608
                     /gene="rarD"
                     /locus_tag="ACHKIR_002380"
     CDS             38718..39608
                     /gene="rarD"
                     /locus_tag="ACHKIR_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HFO9990113.1"
                     /translation="MDTKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPKKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFTFI
                     WVALAIFVMDAIYTQRRTAK"
BASE COUNT         9678 a        10553 c        10042 g         9496 t
ORIGIN      
        1 tcgagtgccc acacagattg tctgataaat tgttaaagag cagtgccgct tcactttttc
       61 tcagcggcgc ggggtgtgca taatacgcct tcccgctaca gagtcaagca tttttttgct
      121 tttctctgtt gagattctca ggagaacccc gctgacccgg cggcgtgttt gccgttgttc
      181 cgtgtcagtg gtggcgcatt atagggagtt attccggcct gacaagagga aatttaaaat
      241 aattttctga ccgcacattt tttattcttt atgcttattt cagcgtcggt ttgtcggtta
      301 aatgggcgat ttctcgggcg aaattcgcca cctgctccca atcggtatag acaacttctt
      361 tgcgcgtatc cgtttcaccg cctgacatct tcataatcag cttgatcata aaacggtcgt
      421 accagcgata acgtgggtaa cgcagcgccc cggcaatgac ggcacagcga tcgggacgcc
      481 attgtgaact catcagaaac ttccgcgcgt agctgttggt ctgtggagta cgcttctccg
      541 gtttacgcgc caccaggttt acggagtaaa aggcgctcgg catcgaattc agtcgcgtcg
      601 catgcttttt gacaaactcc tggaacgcag agtggtagtg accatagcga atagaagcac
      661 caatgaccac acggtcgtag ttttcccact gtggttcttc aatgcggtgc acattggcga
      721 catccgcctg gatccccagt tctttcagtt ccgaagccag gtaggaggca atctcgcgcg
      781 tttgtccgtc ccttgttgag aaaagaatta atgttttcac gtattactcc atcattcacg
      841 ccagaaagtc ggggtaaaga gcaccagcaa tgtaaagacc tcgagacgac caaacagcat
      901 gttggcaatc aggatccatt tagccaccgg gttcatactg gtaaagttat cagcaaccac
      961 accaagccct ggccccaggt tattcaatgt cgcaacaacc gaggcaaagg cagaaaagtc
     1021 atccacgccc gtggcgataa tcgccagcat actgacaata aacaccaatg cataggcgga
     1081 gaaaaatccc cacacggctt cgaggatacg ttccggcagt gcgcgattcc ccagcttaat
     1141 gctatagacg gcgttcggat gcaccagtcg tttcagctca cggttcccct gcttaaacag
     1201 cagcaggatg cggatcactt tcaggccacc gcccgttgac ccggcgcaac cgccgataaa
     1261 agctgaacat aagagcagta ccggcaaaaa gagcggccag cgggcaatgc tgtcagtcgt
     1321 aaacccggcg gttgtcgcca tcgataccac ctggaaaaac gcctggttaa ttgtcatcag
     1381 cgccgaactg tagacattat gaaaccacag tacgagtgta caaataacca ccagcgtaaa
     1441 ctgtacgccg ataaacatgc gaaattccgg atcgcgccaa taaaccttca gactacgccc
     1501 acttaacagt gaaaagtgca gaccgtagtt acagccggag atcagcagga agatagcaat
     1561 gatagtgtta atagtcgggc tgtcgaaata accgatactg gcatcatgtg tcgagaagcc
     1621 gccaatagcg atagtcgcaa agctatggcc gatggcatca aaggcatcca ttccggcaaa
     1681 ccacaacgcc agcgcacagg cgacggtcag caagacataa atcaaccaca gggttttcgc
     1741 cgtttccgca attcgcgggc gcattttgtt atctttcagc gggccgggca tttctgcgcg
     1801 atagagctgc atcccaccca cgccgaggat aggcagtatc gcaaccgcta acacgatgat
     1861 ccccatcccg ccaaaccatt gcagcatctg gcgataaaag aggatggcgt gagggagcga
     1921 atccagcccc actagcgtag tggcccccgt ggtggtcagg ccagagaaag attcaaaaaa
     1981 cgcatcggta atcgtgaggt tcgggctttc cgagaagata aaagggagcg caccgacgct
     2041 acccagcacg gtccagaaca gcaccactat cagaaacccc tcacgggatt tcagttcgcc
     2101 cttctctttg cggttcggcc accacagcat agagccaatg gcgagggcga caaaaaaggt
     2161 ctgggtaaaa gcgcggcccg ctccatcccg gtagatgagt gctaccagcc cagggataat
     2221 catggtccct gaaaataaga tgaccagtag tccaacgatt cgggtaatgg cgcgaaaatg
     2281 catctctgcc gcttccttag ttctgcaaaa cgaagtgggg attattcttc aatcgctaac
     2341 aattgcaatg aaccacggct aaaatccgcc agctttgcgg aaaattcagc cactttcgcc
     2401 gccggaagcg ccacccgcag cagaacgaat gcctgataat cactgttaat aattttgccg
     2461 tcacactgcc ccagcaacgc ttcaatgccg gttaactgac tatattcaca ttgcaaagta
     2521 tattcggtta atggtgtctt gcgttgggtc gttagctggc gcagcgcctg attcacgccg
     2581 ccgccatacg ctttcactaa cccaccggtg ccaagcaata tgccgccgta gtagcgcacc
     2641 accacagcgg taatttcccc gacgccgctg cccattagtt gggcgagcat cggtttacct
     2701 gccgttcccg ccggctcccc gtcgtcagag aaacccagct gttgagaatc atccggcgca
     2761 cccgccaccc acgccacgca atggtggcgg gcatcggggt gctctgcccg caccgattca
     2821 acaaacgctt ttgccgcctc aacgccatcg gtatgcgcca acagcgtaat gaagcggctc
     2881 tttttgatct cttcaacgac cgtgaccggt gccgcaggaa ttaaccagct ttccatcacg
     2941 ccagtttcag atcccgggta atgttttcca cgttgttttc gtgaatcacc acgttgtctt
     3001 cgatacgaat gccgccgaac ggtttcagtg cttcaatttt ctgccagttg aagtgtttgc
     3061 tgaactgccc ttcacgccac ggtgccagaa gcgattcgat gaagtagata cccggttcga
     3121 tggttaacac catgcccggc tggagaatac gggtgcagcg caggtacgga tattttgccg
     3181 gtgccgcgag gtgcgtaccg ctatcatcct gcataaaacc ggctacgtca tgcacctgca
     3241 ggcccagcgg atggccgata ccgtgcggca taaacggtcc ggtgagatcg ttttcgacca
     3301 tcgcttcttc actcatatcg gtgatgattt gatgtttacg cagcagtttg gcgatgcgct
     3361 gatggaactg gatatggtaa tccacatagc tgacgcctgc tttcatggta gcgatcagcg
     3421 ccagttgttc atcatttacg tctttcacca gctgtgcgta gtcgttgtcg ctttttgccg
     3481 accaggtacg ggttagatca gccgcatagc cgttatattc ggccccggca tccagcagga
     3541 agctgcgcat ctcttccggt gcctggtggt ccagtttggt gtaatgcaac accgcagcgt
     3601 gttcgttaag cgccacaatg ttgctataag gtacgtcggt atcacgatga ccggtcgcgg
     3661 tcagataggc aatgttgata tcgaactcgc tcatgccaga acggaacgct tcttctgccg
     3721 cgcgatgacc gttgaccgcc attttctgcg cttcacgcat acaggccagt tcgtactcgg
     3781 ttttgaagga gcgatagtaa tgcaggtagt cgatcacccc tttcgggttg atattgctgg
     3841 cctcaatacc cagttgcagc gcacgttctg gcaccggacc gatataaccg atattgccgc
     3901 gcgcagcagg caacagacta ccaatgccat cggctttcgg cagcgcgatc acttctacat
     3961 cttcagtcca gaaggagttc ggcagcggtt cgacgttgtg ccagtaatca accggtagat
     4021 agaaccacag tttcggctta ttcacgccat ccaccagcag ccagcagttt ggcacctgag
     4081 ttactggcac ccacgctttg aattgcgggt tcactttaaa cggataggga tgatcgtcga
     4141 gaaaaacgtt gaacagttcg ccggagtgaa taagtaacgc atccagcttg aagcgcgcca
     4201 gcgcatcgcg agtccgttcc tgtaaggtag ctatatgatt tttatagagc gaggccagtg
     4261 attccatttt ttacccttct gtttttttga ccttaagtct ccgcatctta gcacatcgtt
     4321 catccagagc gtgatttctg ccgagcgtga tcagatcggc atttctttaa tcttttattt
     4381 gcatattttt aacacaaaat acacacttcg atgcatctgg tacgaccaga tcaccttgcg
     4441 gattcaggag actgacatgc tttacaaagg cgacaccctg taccttgact ggctggaaga
     4501 tggcattgcc gaactggtat ttgatgcccc aggttcagtt aataaactcg acactgccac
     4561 cgtcgccagc ctcggcgagg ccatcggcgt gctggaacag caatcagatc taaaagggct
     4621 gctgctgcgt tcgaacaaag cagcctttat cgtcggtgct gatatcaccg aatttttgtc
     4681 cctgttcctc gttcctgaag aacagttaag tcagtggctg cactttgcca atagcgtgtt
     4741 taatcgcctg gaagatctcc cggtgccgac cattgctgcc gtcaacggct atgcgctggg
     4801 cggtggctgc gaatgcgtgc tggcgaccga ttatcgtctg gcgacgccgg atctgcgcat
     4861 cggtctgccg gaaaccaaac tgggcatcat gccgggcttt ggcggttctg tacgtatgcc
     4921 acgtatgctg ggcgctgaca gtgcgctgga aatcattgcc gccggtaaag atgtcggcgc
     4981 ggatcaggcg ctgaaaatcg gtctggtgga tggtgtagtc aaagcagaaa aactggttga
     5041 aggggcgatg gcgattttac gccaggccat taacggcgac ctcgactgga aagcgaaacg
     5101 tcagccgaag ctggaaccgc taaaactgag caagattgaa gccaccatga gtttcaccat
     5161 cgctaaaggg atggtcgcac aaacggcggg gaaacattat ccggccccca tcaccgcagt
     5221 aaaaaccatt gaagcggcgg cccgttttgg tcgtgaagaa gcattaaatc tggaaaacaa
     5281 aagttttgtc ccgctggcgc ataccaacga agctcgcgca ctggtcggca ttttccttaa
     5341 cgatcaatat gtaaaaggca aagcgaagaa actcaccaaa gacgttgaaa ccccgaaaca
     5401 ggccgctgtg ctgggcgcag gcattatggg tggcggcatc gcttaccagt ctgcgtggaa
     5461 aggcgtaccg gttgtcatga aagatatcaa cgacaagtcg ttaaccctcg gcatgaccga
     5521 agccgcgaaa ctgctgaaca agcagcttga gcgcggcaag atcgatggtc tgaaactggc
     5581 tggcgtgatc tccacaatcc acccaacgct cgattactct ggttttgacc gcgtggatgt
     5641 tgtggtagaa gcggttgtcg aaaacccgaa agtgaaaaaa gccgtactgg cagaaaccga
     5701 gcagaaagta cgcccggata ccgtgctggc gtctaacact tcaaccattc ctatcagcga
     5761 actggccaac gcgctggaac gtccggaaaa cttctgcggg atgcacttct ttaacccggt
     5821 gcaccggatg ccgttggtgg aaattattcg cggcgagaaa agctccgacg aaaccatcgc
     5881 gaaagtcgtc gcatgggcga gcaagatggg caagacgccg atcgtggtta acgactgccc
     5941 cggcttcttc gtcaaccgtg tactgttccc ctatttcgcc ggtttcagcc agttgctgcg
     6001 cgacggcgcg gatttccgca agatcgacaa agtgatggaa aaacagtttg gctggccgat
     6061 gggtccggca tatctgctgg atgttgtggg cattgacacc gcgcatcacg cccaggctgt
     6121 catggcagca ggcttcccgc agcggatgca gaaagattat cgcgatgcca tcgacgcgct
     6181 gtttaatgcc aaccgctttg gtcagaagaa cggcctcggt ttctggcgtt ataaagaaga
     6241 cagcaaaggt aagccgaaga aagaagaaga tgccgtcgtt gacgacctgc tggcagaagt
     6301 gagccagccg aagcgcgatt tcagcgaaga agagattatc gcccgcatga tgatcccgat
     6361 ggtcaacgaa gtggtgcgct gtctggaaga aggcattatc gccactccgg cagaagcgga
     6421 tatggcgctg gtctacggcc tgggcttccc tccgtttcac ggcggcgcgt tccgctggct
     6481 ggacaccctc ggtagcgcaa aatacctcga tatggcacag caatatcagc atctcggccc
     6541 gctgtatgaa gtgccggaag gtctgcgtaa taaagcgcgt cataacgaac cgtactatcc
     6601 cccggttgag ccagcccgtc cggttggcga cctgaaaatg gcttaaggag tcacaatgga
     6661 acaggttgtc attgtcgatg caattcgcac cccgatgggc cgttcgaagg gcggtgcttt
     6721 tcgtcacgtg cgtgcggaag atctctccgc tcatttaatg cgtagcctgc tggcgcgtaa
     6781 cccggcgctg gaagcggcgg ccctcgacga tatttactgg ggttgtgtac agcagacgct
     6841 ggagcagggt tttaatatcg cccgtaacgc ggcgctgttg gcagaagtgc cgcactctgt
     6901 cccggcggtt actgtcaatc gcttgtgtgg ttcatccatg caggcactgc atgacgcagc
     6961 acgaatgatc atgatcggcg atgcgcagac atgtctggtt ggcggcgtgg agcatatggg
     7021 ccatgtgcca atgagtcacg gcgtcgattt tcaccccggc cttagccgta atgtcgccaa
     7081 agcggcgggc atgatgggct taacggcaga aatgctggcg cgtatgcacg gtatcagccg
     7141 tgaaatgcag gatgcctttg ccgcgcggtc acacgcccgc gcctgggccg ccacgcagtc
     7201 gggtgcattt aaaaatgaaa ttatcccgac cggtggtcac gatgccgacg gcgtcctgac
     7261 gcagtttaat tacgacgaag tgattcgccc ggaaaccacc gtggaagccc tcgccacgct
     7321 gcgtccggcg tttgatcccg tcagcggtac ggtaacggcg ggtacatctt ctgcgctttc
     7381 cgatggcgca gctgccatgc tggtgatgag tgaaagccgc gcccatgaat taggtcttaa
     7441 gccgcgcgcc cgtgtgcgtt cgatggcggt cgttggttgt gatccgtcga ttatgggtta
     7501 cggtccggtt ccggcctcaa aattggcgct gaaaaaagcg gggctttctg tcagtgatat
     7561 cggcgtgttt gaaatgaatg aagcctttgc cgcgcagatc ctgccatgta ttaaagatct
     7621 gggactgatt gagcagattg acgagaagat caacctcaac ggtggcgcga tcgcgctggg
     7681 tcatccgttg ggctgttccg gtgcgcgtat cagcagcacg ctgctaaatc tcatggagcg
     7741 caaggatgcc cagtttggtc ttgcgacgat gtgtatcggt ctgggtcagg gtattgcgac
     7801 ggtgtttgag cggatttaac cacttgccag atgcggcgtg aacgccttat ccggtctacc
     7861 gatcctgcac caattgtagg cctgataaga cgcgccagcg tcgcatcagg cagcgtgtgc
     7921 cgaatgccgg atgcgacata gatctaaatg ccactgtata aagttttagt tgccgttctt
     7981 cccgcctgtc aggggcgggt tttttatatc tcagataaat gcaaacgcat cgccaaacag
     8041 gcgatcttcc cgcgcattac gctcgctgca aaacaggtca cgggcaattt tcgccatctc
     8101 aaaacgtccg gcaatataga tatcatgctc tgccagcgta ccatgatcct gcaataccgc
     8161 cgttaacacg gtgccagtac gcccacgcca gcccgcttcc ggttgttcaa ccaccggcac
     8221 cacttgtaga ccaggatgct tcaacgaaag cgcctcaagc tcgcagagat catacagatg
     8281 ctgctcttca cgcccgcccc agtaaatggt gatatcacgg tttgggttac gcgccaacgc
     8341 tgtcagcaaa atcgagcggg cataagagaa cccggtgccg cccgcaatca aaatcatcgg
     8401 acgctcttca tcatcgcgca gccacgcttc tccgtgggga atatcgacca cgatttgatg
     8461 atctttgagg atgcggtcca tgactgcttt cgcgtaaagg ttgatttcag aagcgccaat
     8521 atgcagctcg ataaaccctt tttcatctgg cgtcgaagcc attgagaacg gacgtttgtc
     8581 gcgctcatcc atcactacca tcaaatactg accagcacga aaagaaaagg ccgcgtctgg
     8641 cacgatgcgg acacgatata cggtatccgt gatagcttct accgaggtca ctttacagct
     8701 taaggttgtc atgcgctttc tctgtcggat cgataaatag ggcaaaacaa acgcgcatca
     8761 ggcgctttta ccgttgttaa aaatagccag ttcatcccaa atggcgtcaa tatgcgcgac
     8821 aacatctgga tcttttttga tgggacgtcc ccattcacgc tgggtttccc ccggccattt
     8881 attcgtggca tccagcccca tttttgaacc cagcccggag acaggggagg caaaatccag
     8941 ataatcaata ggcgtatttt ctaccagaac ggtatcccgc gccgggtcca tacgggtggt
     9001 aatcgcccaa atcacatcgt tccagtcgcg ggcgttgacg tcatcatcgc aaacgatcac
     9061 aaatttagtg tacataaact ggcgtaagaa cgaccagacg cccatcatga cgcgcttcgc
     9121 gtgtccggca tactgttttt tgatcgtcac taccgccaga cgataagaac agccttccgg
     9181 cggcagataa aaatcgacaa tttccgggaa ctgtttttgc agaatcggca cgaacacttc
     9241 gttcagtgct acacccagta ccgcaggttc atctggcgga cgcccggtat aggtggaatg
     9301 ataaatcgca tcttcacgct gggtaatatg cgtcacggta aataccggaa aactatcgac
     9361 ttcgttatag taaccggtgt ggtcgccata cggcccttcc ggcgcagttt cgccttgttc
     9421 gatataccct tccagcacaa tctccgcact ggcgggcact tcaaggtcat tggagataca
     9481 cttcaccact tcggtcttgg tgccgcgcag caatccggca aacgcatact ctgaaagcgt
     9541 atccggaacg ggggtgactg caccgagaat cgtggcggga tcggcaccca gcgccacaga
     9601 aaccgggaaa cgttcgcccg gatgcgccgc acaccactcc tgataatcca acgcgccgcc
     9661 gcgatgcgac agccagcgca taatcagttt gtttttacca attagctgct ggcgataaat
     9721 gcccagattc tgccgctctt tatgcgggcc gcgcgttacg gtgagccccc aggtaatcag
     9781 cggcgcggca tcttccggcc agcaggtcat aatgggaata cgattgagat cgacgtcatc
     9841 gccagagacg attttttgtt ggcaaggcgc accacgcagc cgctttgtcg gcatgttcaa
     9901 tacttgctta aactgcggca gtttatcaaa caggtcgcgg aaaccttttg gcggctccgg
     9961 ctctttcaga aacgccaata atttaccaac ttcacgcagc gccgaaacat cttcctgccc
    10021 catgcccatc gccacgcgct ttggcgtacc gaacaggttg cacagcaccg gcattgagta
    10081 gcctttaggg ttttcgaaca acagcgcagg cccaccggca cgcaaagtgc ggtcagcaat
    10141 ttcagtgatt tccaggtgcg gatccaccgg gagcgtgata cgttttagct caccctgctg
    10201 ttcaagcagc gtcaagaagt cgcgtaaatc gttatatttc atggcgtcca ttgtagcctc
    10261 ttaatctgcg cccattatac ggcgttcatc tttgcgatgc tgtaaatttg ttaaattagc
    10321 gtgaactctg acggtataac gcaaaccggg gaatataatt aacttagcgt aaagcttttg
    10381 ctatccttgc gccccgatta aacggataag agtcattatg caatcctggt atttactgta
    10441 ctgcaagcgc gggcaacttc aacgtgccca ggaacacctc gaaagacagg ctgtgaattg
    10501 cctggcaccg atgatcaccc tggaaaaaat cgtgcgtggc aaacgtactg cagtcagtga
    10561 gccattgttc cccaactact tgtttgtgga gttcgatccg gaagtgattc ataccacgac
    10621 tatcaacgcg acccgcggtg tcagccactt cgtgcgcttt ggcgcgtcgc cagcgatagt
    10681 cccatcggcg gtgattcatc agttatcagt gtataaaccg aaagatattg tcgatccatc
    10741 gacgccgtat cctggtgata aagtgattat tactgaaggc gcattcgaag gttttcaggc
    10801 cattttcacc gagccagacg gcgaggctcg ctccatgcta ttgctgaatc ttattaataa
    10861 agagattaag cacagtgtga agaacaccga gttccgcaaa ctctaaaacg caatcccaaa
    10921 cagtgttttg acattagcat ccgtggtggc agccagccat gcggcatctt ctccacgcca
    10981 gtgcgcaata cgttgcaaaa tatggggcag atgggctggc tcgttgcgcc gggatgatgg
    11041 ctttggcgtg agatcgcgag ggagcagata cggcgcatca gtttcgatca gcaatttctc
    11101 cgccggaatc aacggcaaca attcccgcag ctccagcccg cgtcgttcat cgcaaaccca
    11161 accggtaatg ccgatataaa ttccatgcgc cacgcacgcc tgcatctctt cgcgtgtgcc
    11221 ggtaaagcaa tgaagaaccg caccaggcac tttatccagc cacggctcca gcaatgtcat
    11281 aaaccgctcg tgggcatcgc gacagtgcat aaataccggc atgtttaatt ctgcggcaat
    11341 gcgtagctgg gcaacaaaag cgcgttcctg ttcttccggc gtcgaaaagt tgcggttaaa
    11401 gtcgagacca cattcaccaa tcgccaccac ttctggctgc gcggccagct caataatcgc
    11461 ttcttcagtc gcagcttgcc actggctgct gtcgtgagga tgtacgcccg ccgttgacca
    11521 acaggacgaa tactgacgcg ccagcttttg cgcctgctgg ctttcacgca ggttagtgcc
    11581 ggtgatgagt agcccattaa ctcccgcgtc aaaagcgcgc gctacaacat catcacggtc
    11641 tttcgcaaat tgcgaactgg tcaaattaac gccgatatca aacatcctgt actccatatg
    11701 acaaccgccc tgacgggcgg ttgaatttat tcttcagttt tttcgctttc tgcttcagcg
    11761 tcgttttcct cttcccgatt tcgcccttta ccaacgtaaa agcgtgagaa gaagacaccg
    11821 atttcaaaca ggcaatacat cgggatcgcc agcagcgttt gcgagaagac atccggcggc
    11881 gtcagcagca tcccgacaac aaatgcgcca accagcacat acggacgttt tttgcgtaag
    11941 tcttctggcg aggtaattcc catccagcac agcagcacta ttgccaccgg cacttcaaag
    12001 gagacaccaa acgccataaa cagcgccata acgaagctta aatagctggc gatgtcggtg
    12061 gatacctgta ccccttccgg cgcggtattg gcaaggaagc caaatgccag cggaaagacc
    12121 acaaagtagg cgaacgccat gccgatataa aacaacagag agctggaaac cagcagcggc
    12181 accaccaggc gacgttcatg cttatacagc gctggggcga taaacgccca cacctgatag
    12241 agaatcaccg gcgctgacag aatcagcgac accataaagg tcagcttgat cggcgtaaag
    12301 aacggcgagg ccacgtcggt ggcgatcatc gttgaacctt gcggcaactg cttgatcaat
    12361 ggcgcggata ccaggtgata gatgtcattg gcgaaataga ccagacacag gaatatcacg
    12421 atcaccgcga taatgcagtt cagcagacgc ttacgcagct caatcagatg cgtgataagc
    12481 ggttgagtat cttctacaga catgtttacg gtttatcact cgacgaaggg gaaggtgcag
    12541 cggttttcgg ttcagcgtcc gcagcaggtt ttaccaccgg ctctggcgtg gtttctggct
    12601 tctgttccgg cgaactggct tgcgtttgtg cagcagcagg cgttacgcct tcatgcgcag
    12661 cttcattgtc tttcaccacc gggttgtgga tggtgtgcgc ttcatcgctc gccttttcag
    12721 gatcgtttgc aacgtaggaa cgcttcatcg actccgcggc ctggcgtagt tcatccatcg
    12781 acgctttcag ttcgggcgtc aggttagtga ggctcgcctt ttcaaccttt ttcagactgt
    12841 cctgaaactc ctggagtttt aactcctggg tcagttcgtt ctgcaccgtt gtcgccagtg
    12901 aacgcaacgc gcgaatccag cccgcgaccg tttttaccgc cacaggcagt cgttgcggcc
    12961 ccagaacgac gaggccgatg atgaatacca atagcagttc gctaaaaccg atatcaaaca
    13021 cggattacac ctgctcttta tcgtggcgct tcgcgtcttc tgttttagcc tgttcctgat
    13081 tcgtatccgc ctgcttatcg gcgatagttt tcgcagtaaa atcagcatcc tgactggttt
    13141 tatcctgctt tggttcatca tcgctcattg cttttttaaa gcctttgatc gacgcaccaa
    13201 gatcggaacc gatggagccg agctttttgg tgccaaaaag cagtacaacg atgacggcaa
    13261 taatcaataa ctgccaaata ctgataccac ccatacatgt tcctctgtgg tagatgatga
    13321 ttaaacaagg ccgcattata cgttacacgg cccgccttga gcgatgaaaa aatcagcgtg
    13381 ttttgcgcca accgacaaac caggcgatca gaccacctgc cattaaccag ccgggcatca
    13441 gcccccattc aggtcggctg accaacaaga atgtgccact taatactaac gtagcgccaa
    13501 ttccgagaaa ataacgcgat tgtccctgac gtacatgatt tgactgaagc tcgcgggcaa
    13561 tcttatcaac actgtgctgt aaatacttgc cctggcgcaa actgtcgtaa accagttcag
    13621 gcagttctgg cattttttcg acccagaacg gcgctttttc tttaaatgct ctcaccagcg
    13681 caggaatacc gacctgatct ttaatccacg actcaaggaa aggcttcgcc gttttccata
    13741 aatcgagctg cggataaagc tggcgtccta ccccttcgac gtagagcagg gttttctgga
    13801 gtaacaccag ttgcggctgc acttccatat tgaagcgacg cgccgtatta aatagattta
    13861 acagcacatg tccaaacgaa atttcggcca acggtttctc aaagataggt tcacagaccg
    13921 tacgaatggc aaattcgaac tcttcaacgt tggtatctgg cggcacccag cctgaatcga
    13981 cgtgtagctc tgcaactttg cgataatcgc gattaaagaa agcgataaag ttttccgcca
    14041 gatagcgttt atcttctttg tttagcgagc caacaatccc gcaatcaatg ccgatatatt
    14101 tcgggttttc tgggtgttca tagcttacaa agatgttgcc agggtgcata tcggcatgga
    14161 aaaagctgtc gcgaaagacc tgagtgaaga acacctgcac gccgcgttcc gccagcaatt
    14221 tcatgttggt gccgtttttc tccagcgtcg caacatcaga caccggaatg ccgtaaatac
    14281 gctccatcac catcatccct tcactacaat agtcagggta aacttccggg atgtagagca
    14341 tcgggctgtc ttcaaaattg cgtcgaagct gaatggcgtt ggcagattcc cgcagcaaat
    14401 tcagttcatc aatcaaggtc ttttcgtact cgcgcaccac ttcggttggg cgcagacggc
    14461 gaccatccgg cagcaaacgc ggcacccagc gagccagacg gtagataagt ttcagatccg
    14521 ctttaatgac cggcaaaata tccgggcgga tgactttaat caccacctct ttaccattcg
    14581 atttcaatcg cgcggtatga acctgggcga tagaagcaga agccagcggc ttgatttcaa
    14641 aatcgtcaaa ccacgcttct accggcaagc cgcccattgc agcttcaatc tgctgcttcg
    14701 ccagcttgcc atcaaacgga gcgactttgt cctgcaataa cgccagctga tcggcaatat
    14761 gcggcggaaa aagatcgcgg cgggttgata acatctgccc gaacttgatc cataccggtc
    14821 ccagttcttg cagggccagt cgtagtcgct cacctaaagg tttgtcttta tgccgatttg
    14881 gcatccagaa taatgagtat cgccatagcc gtagcggcag ggtgatacgc attttgggga
    14941 tcagttcatc aagtccgtag cttaaaaaag tgcgaatgat gaaatatagg cgccgtactt
    15001 cacctggcgt catttagcct ccagtttttc cagccgtttg gtcagggcat caacagcacg
    15061 ctcgacggca gccgtctctt ccgcaaacca ggccacttca agcggaccgg gtgccatacg
    15121 ccactcttca gtaatggctt ccgccacata acgttgctgg cgcttaatgc cgtgatgcag
    15181 gaactttgcg cccccgcgca gggctttgct gataccttca gcggcgatat caccggtata
    15241 aggggccagc agttccgcag ggtcgaactc agccagatct gccagcgcaa cgaagttttg
    15301 caccacctga atatcgccct gcacttccag ctcaccactg cgaatcagtg cggcaagctg
    15361 ctggcgatcg cgaagtttcg gcaacacact ggcgtaggcg ataacggtgc agtcagcatc
    15421 gcctgcccat tcgcccagta catcaacctg gcgttcgctg aacaccagaa tcaatgacgt
    15481 cgaaaagcct tttacctcca cgcgcagcac tttgcccaac agacgcgagc gggccgtttt
    15541 cagcgcgggt gagcgataca ggaaggtgtt gagcagactt tcaattcctg ccgtcactaa
    15601 aggtttaaaa ggcatttccg gtctcctgtc agaacttata accacgatgc agcgccacaa
    15661 cccctgccgt cagattgtag tagtcgacac tttcgaatcc ggcatcctgc atcatggttt
    15721 tcagggtatc ctgatcggga tgcatacgga tggattctgc cagataacgg tagctgtcgg
    15781 cgtcgttcgc gaccagtgag ccaatacgcg gcagcacatg gaaggagtat gcatcatagg
    15841 ctttgctcag cggctcgata attggcttcg agaactcaag caccagcagg cggccgccgg
    15901 gtttcagcac gcgatacatt gaacgcagtg ctttatcttt gtcggtgacg ttacgcagac
    15961 caaacgaaat ggtgatgcaa tcaaaggtgt tatccgggaa cggcagcgcc tcagcgttcg
    16021 cctgaacata ctcaacgttg ccaatcacac cgatattacg cagcttctcg cggcccattt
    16081 tgagcatgga ttcattgata tcagcaagga ccactttgcc agtttctccg accaggcgag
    16141 agaatttcgc tgtcaggtcg ccggtgccac cagccagatc cagcacggtc tgcccacggc
    16201 gtacgccgct gcaatcaatc gtgaatcgct tccacaaacg atgaataccg aatgacatca
    16261 aatcattcat gacatcgtat tttgatgcca cggaatggaa aacgtgggcg accatatccg
    16321 ctttttgttc cttcgcgacg gtctgaaaac caaagtgcgt cgtttcttgt gacttatcca
    16381 ccatctcaat gcctgctcat caaaaaattg ttccagaagt gtaacagatt gggcgtcgat
    16441 gccctagatt tctacccggc ttaactaccc ccaatgggct agcgcgactg ctgattatat
    16501 tcatcatcgc gttgataagc ttcatcattc ggctgctccg gaaccgaccg aagtcgatac
    16561 tcttcatcct ggctcaccgc ctgttcagcc aaatccggat taatctcgcg tttaatttct
    16621 acccctaaac cgcgaaacgc ttctgcctgc gccagcacat ttccgcgacc tgaagagagt
    16681 tttttcattg cctggcggta gttatcctgc gctttgtcga gactttgacc aatcgctgac
    16741 atgtcatcga cgaacaaacg catcttgtcg tacagcttgc tggcacgatc ggcgatttgc
    16801 tgggcgttgc ggctttgatg ctcataacgc cacaggttgg cgatagtgcg cagcgccacc
    16861 agcagcgtag tcgggctaac cagcatgata ttgtttttca acgcttcggt gatcagctcc
    16921 ggctggcggt caagcgccag taaaaaagcg ggttcaacgg gaataaacat cagcacgtaa
    16981 tccagggttc gcagccccgg cagctgttga taatctttgc gtcccagcaa acggatatgg
    17041 ttacgcaccg acgcgatatg ttcctgtagc gcgctttcgc gggtgtagtc atcttcggcg
    17101 ttgaaatagc gttcataggc gaccagcgtc attttggcgt cgatcaccac atcttttccc
    17161 tgcggcaacc gcacgatgac atccggctgc atccgcgagc gggcgtcatt ttcgatgctg
    17221 acctgagttt catattcata cccttcacgc agaccggaag cctccagcac ccgcgtcaat
    17281 accacctcgc cccagttgcc ctgggtttta ttgtcgcctt tcagcgcgcg cgtcaggttg
    17341 atcgcctcct gagccatttg cgcgttgagc tgctggagat tgcgaatttc gtgggtcagg
    17401 gtatgacgtt cctgtgcttc tttaccgaag ctgtcctgaa cctgacggcg gaaaccgtcc
    17461 agttgttcac gtagcggcga caacaggctg ttcagactct gacggttttg ctcatcaacc
    17521 cggcgattgc tgtgctcaaa aatacggttg gcgaggtttt caaactgctc actgaggcgc
    17581 tgctcgctgt tgatcatctg gcgaatttta tcgtcagcat gttgctgtgc ggcttccatc
    17641 cgcgtggtta cttcacgcag atcggcctcc agagaggtgt taatactttg caggctgcgc
    17701 acttcgttat tgagtaactc gcactcagcg cgccagtgct cgctttgagt aatttgttgt
    17761 tttgccgcgc ttaactccgc gaccatctct tcacgttcag ctaattgctc ggctttttgc
    17821 tgcgcatgtt gataactggc aaacagccag ccaatgacca cactaaccag cgcaataatg
    17881 gcataaacaa tgattgagaa atccacaaag cctcctgacc caacaactta cccgcacaaa
    17941 atagaattgt gctgtacaaa cgtccagttt gaaagaagtt tcctgcgagg cgctacgcaa
    18001 aacgatcgtt aattttcatt attttatccc acaatatgag atggagttta tattgtggga
    18061 tatatcacct tttccccacg tcattatgtg gaacacttca gcaatatcat caggagaaac
    18121 gtatggcaga aacctcttca accaccgcag gcgcacaaac gttattacgt ggactggcgg
    18181 ttcttaatgc tgtttataac ggctgccatg acctaaaaag tatcggcgag ttcaccggca
    18241 cgacccggag cacaacccat cgtttagtca ccgttctggt ggaacaacgc tacctgcgcc
    18301 atgtcccaac gcagggctac caactgggtg ccaaactgat cgaattcggt gccagagcac
    18361 tggaaagcac ctcactgtac gaaattgccc agcccgtttt gcaacgcctt gcccgttaca
    18421 ccctggacac cgtgcatctg gggatcgttg aaggcgacga agtgctgtat ctggagaaga
    18481 tcaacagtca gcgcgggctg gaaatgcgct cacgcccagg ccatcgcatg ccactggcaa
    18541 tcaccggcat cggcaaggca ttgatcctca accgaacaga agaagagtgg cgcacgctgt
    18601 ttaagacctg tggtgatgaa acaaaactgg gcgcatttat tcagaacatg cgtcgttatg
    18661 ccgccagtgg ttttgctttt gatctggaag agaacgagcc gactatccgt tgcgttgcag
    18721 cgccggttta caacgcccgg gatgaaattg tggcggctat ttctgtcgcc agtaccacca
    18781 cgtatatgtc gctggcgcgt ctggaagaac tggcacctta cgtcaaatcc tgtgcagaag
    18841 agatatccgc tgaactgggc tggggtaagc acgtccgcaa agataaataa accatgatta
    18901 aaggaaacga ctaatgccgg gagcaccctc atttattgcc ctggactggg gaacgtcatc
    18961 gcttcgggca tggcgttttg gcgaatcacc aaatccacaa gaaaaacgtg aattcccctg
    19021 ggggatcatg aaattaccct cccaggcagc aacacgtgag gacgctttcc acgatacgtt
    19081 tttacgtgtc tgcggcgact ggctggcaca aaccccatgc ccggtactgg cttgcggcat
    19141 gctcggcagt gcgcaagggt ggcaatcagc agcttattta ccttgcccgg tcactctcga
    19201 gggtctggcg aagcagttga cgcctgttat ccaccagcag cagacaatgc tgcacatcat
    19261 tcctggcgtg attaaagaag gtgaaatgcc cgaagtgatg cgtggcgaag agacacaaat
    19321 cttcggtgct atctcgatgg aaccggccct gcaaaacgcg atccatcagg gtatgccagt
    19381 gctgataggc ttacccggca cacatgcgaa atgggcagta gttgaaaaca acaccattac
    19441 cgatttccga acctttatga cgggcgagtt atttgatgtt ttatcccgcc attcgattct
    19501 cggtgccacc atgcatcctg gagatgaacc gcactgggat gccttcaccc acgggctgac
    19561 agcagcacaa gagcatcatc agaccggatt attatcgacg ctgttttcga cccgttcgcg
    19621 cctgctgacc agtaatctta cgtcgtcctc gcagggggat tacctttccg gattactgat
    19681 aggccatgaa ttatgtggtc tggcatccag tttgctacgt gatttacccg ccacaacacc
    19741 gatcgcctta atcggtagcg caaatctgaa cagccgctat tcacaagcat tcagccacgt
    19801 ttttcccgac agacagatac acgccattcc taatgccact gaacatggat tatggcgaat
    19861 cgcccacgct gccgggttac tgtccaccaa cgccagggaa tgtacccatg ccatttaata
    19921 cacttttgca aaaaaccggg ttagtcgcca ttttacgcgg cgtgaaaccg gatgagattg
    19981 tcgccatcgg tgaaaaactg tacgccgccg gattccgcct gattgagatt cctatgaact
    20041 cgccggaggc gttgcaaagt atcagtttac tgcgcgacgc cctgcctaag gactgcctgg
    20101 tcggagccgg aactgtgctt tcggtagagc aggtggttgc ggttaaagag gctgggggcc
    20161 agattatcgt gatgccgcat tgcgatactg cggtgatccg tcgcgcccgt gcgctgggta
    20221 tgtattgtgc gcctggcgtc gccacgccca ccgaagcctt tgctgccatt gaacatggtg
    20281 ctaatgccat caagctgttc ccggctgaac aaattacgcc agaagttacc aaagcctggc
    20341 gtgcggttat tccacaatcc gttccgatgt taccggttgg cggtatcaca ccggaaacca
    20401 tggcgcgtta tctgagccac ggcgcaaacg gctttggcct cggttctgcg ctatatcgcc
    20461 ccggcatgac gccagagcaa gtctatgaaa atgcagtact gtttatgaac gcatggaaca
    20521 atttaaatag caactaactg ataaataagg atataaaagc aatgataaaa catcttacct
    20581 taccgaaaaa aatggctgca atggtacttc ttgcaggcgc tgccctcagc attgcgcctg
    20641 tacaggctgc ctcctaccca accaaacaga tcgagttagt cgttccctac gctgccggag
    20701 gcggtacgga tctggttgcc cgtgcctttg ctgatgccgc caaaaaccat ttacccgtca
    20761 gcatcggggt tatcaataaa cctggcggag gcggtgctat cggcctgagt gaaatcgccg
    20821 ctgcccgccc taacggttac aaaattggtt taggcacggt tgaactgacc acccttccca
    20881 gcctcggaat ggtgcgtttt aaaaccagcg actttaaacc cattgcccgt ctgaatgcgg
    20941 atccggctgc tatcacagtc cgtgccgatg cgccgtggaa tagctatgaa gaatttatgg
    21001 cttactccaa agcgaatccc ggaaaagtac gcattggtaa ctcaggcacc ggagctatct
    21061 ggcatctggc ggtagctgca ctggaagaca aaacgggcac aaagttttct catgtcccgt
    21121 atgacggcgc agcccctgcc attacaggcc tgttaggcgg gcatattgaa gcggtttccg
    21181 taagcccagg agaagttatc aaccatgtga atggcggcaa gctgaagaca ctggtagtga
    21241 tggcggatga gcgaatgaaa accatgcctg acgtcccgac gttaaaagag aaaggcgttg
    21301 atctctccat cggcacctgg cgcggcctga ttgtgtcgca aaaaacgccg caggatgtgg
    21361 tggatgttct ggcaaaggca gcaaaagaga cggctgaaga gcctgcattc caggatgcac
    21421 tgcaaaagtt gaatctcaac tatgcatggc ttgacgctgc cagcttccag acccaaatca
    21481 gcgaacagga aaagtacttt gacgagttgc tgactcgcct gggcctgaaa aaataaacct
    21541 gaggtacggt tgatgaacac aaaacagtct gttgctcaac tcgccgtacc gcaccgcaag
    21601 cgcctttcat caacgatggt ggtggcgctg ttactttgtg tggttgctgg cgcggtgatg
    21661 attaatgccg ctgattttcc agcaactgcc attgaaacgg atcccggtgc aagtgccttc
    21721 cctaccttct atgcctgtgc cctgattgtg ctcgctgtct tgctggtgat acgcgatctt
    21781 ttgcaggcaa aaccagcctc ttgcgccaac gcacaggaaa aaccggcatt caggaaaaca
    21841 gcaacaggaa ttgcggcaac cgcgttttat attgtggcga tgagctactg cggttatctc
    21901 attactactc ctgttttcct catcgtcatt atgacgttga tgggctacag gcgatgggta
    21961 ctcacaccgg gtattgcgct gctgttaacg gcaatcctct ggttgctgtt tgtcgaagcg
    22021 ttacaggtgc cattgcctgt cggcacattt ttcgaataac aggggccagc aatgtttgaa
    22081 agtgaacttt taactcaggg tttttcgaca ttactcaata atcctcaggc gctgctgttt
    22141 gccacttttg gggtgatgct gggtattgtg attggcgctt tgcctggtct gacagcgacc
    22201 atgggtgtgg cgattttgct gcctttcacc tacggcatgg agcctgtttc tggcttgttg
    22261 atgatttgcg gcgtcttttt tggtggcgtc tacggtggtt ctatcaccgc aattttgctc
    22321 aaaattcccg gaacgcctgc cgccgcagcc accgctattg atggttatga gctgacgaaa
    22381 cagggaaaag cagggctggc attgagtgcc gccacgttct cttcctttag tggcggaacg
    22441 ctcagcatta tcgtgctgat gtttctctct ccggtacttg ccagttgggc gctgaaattt
    22501 agtgcctcgg agtccttcgc cctggcaacc ttcggactga gtattattgc cagtatttcc
    22561 ggtgagtcgc tgattaaagg gctgattgcc ggggttggcg gattgctgat cgcaacgata
    22621 ggccttgatc caatgggcgg ttttccacgg tttacaggtg gatttgtgga gctgatgaat
    22681 gtgccattta tcccggtgat gattggttta tttgctgctt cagaagcatt ccgctctatg
    22741 gagcaaaacc agcaaattcg tcagggggcg aaggtggcta tcggcagtct gttactgccc
    22801 tggcaaacac tacgccgcat tgcattaacc attttgcgct catcaggatt aggggttttt
    22861 atcggcatga tccccggtgc gggggcggat attgcagctt ttgttgccta taacgaaact
    22921 cgtcgtttca gtaagacacc agaaaacttt ggtaagggtg aaattaaagc tgtggcctcc
    22981 tgtgaggcag gcgcgaatgg ctgcaccggt ggcgcattgt tacctatgct gacgctgggg
    23041 atccctggcg atgcggtaac cgccatcatg ctgggcgcgt taacgttaca gggaatgcaa
    23101 ccaggcccgc tgatgtttac cgaccacggc gatatggttt atacactgtt tgtcggcatg
    23161 atcttttgtt acttcatgct gctagttctt ggactgctct ctttgaaagt catcggtaac
    23221 gtggtgaaga tccccggcaa tattctcaca ccgatgatcc tcgcactttg tgtggtcggg
    23281 acttatgcgt tgaacaatag cctgtttgat gttggcatta tgctgattgc aggcgtggtg
    23341 ggctatttca tgcagaaagg aggatatccg gcatcacccg tagtgctggc attgattatg
    23401 ggcccgatgg cagaaagtaa ttttcgccgt gcgctgtcgc tttcaggggg ttcactggac
    23461 tttctgtata cccgaccgat cactctggca ttgctaactc tggcagcctt tacgctactg
    23521 acgccaataa tccgcaaaat aatgcgttta cggcgtcagt aactgatatc gctccccgaa
    23581 gttggggagc gttttttatt tcagcagacg acgcgccgct tccaccacga ttttcaccgc
    23641 atggctctcg gtttgtttca tcgtctcagc attcgggatc tcttgctggg tgcggttaac
    23701 gataaccccc gctaccatac cggcacgcag gccctgactt gcacacatgg tcagcagggt
    23761 tgcagattcc atttcatagt tcattacgcc catcgcctgc cactcttcca tagaaccttt
    23821 aaagtgacga actacgcgac cagagtaagt atcgtaacgt tcctgacccg ggtagaaggt
    23881 atcagaagaa gccgtcacac caacgtgagt ggtggcacca attgacttcg ccgcttcaac
    23941 cagcgcagtc gtacattcga aatcagcgac agccgggaat tccagcggtg cgaagtgcag
    24001 gctcgcgccg tccagacgaa cagacgccgt ggtaaccagg acatcaccca cattaatatg
    24061 cggctgaata gcgcccgttg taccgatacg caggaaggtg cgaatgccca gctgtgccag
    24121 ctcttcaaca gcaatagagg tagacgggcc gccgataccg gtagagcaga cgataacagg
    24181 tttaccatcc agctctgcac gccaggtggt gaattcgcgg tgagatgcca gcttaaccgg
    24241 cttatccatc agcgcggcga tcttttccac acgatccggg tcgccaggga cgatggcaag
    24301 cgtagcccct tgtaaatcgt ttttagtgag gccgagatga aaaacatcag acttggacat
    24361 atacaactcc tctgtgaatc ggtttagtca gaagaagcaa aaagacactt taccgaaggg
    24421 tttaacattt tttcgtgata ctcatcacca tgacgcaaat gcgttgcata aatttaccca
    24481 tcaaaagtga tgcaaatcac ataaatcact cttcattctg caggactgca taaaagatga
    24541 gccgattcag gcaatgtgat ttgttgcatg ctgactatag ttaacattgc gcaacatttt
    24601 tataagggta cggagaatac catggcaaca acacaacaat ctggatttgc acctgcggca
    24661 tcgcctctcg cttcgaccat cgttcagact ccggacgacg cgattgtggc gggcttcacc
    24721 tctatccctt cacaagggga taacatgcct gcttaccatg ccagaccaaa gcaaagcgat
    24781 ggcccactgc cagtggtcat tgtagtgcag gaaatttttg gcgtgcatga acatatccgc
    24841 gacatttgtc gccgtctggc gctggagggg tatctggcta ttgcacctga actttacttc
    24901 cgcgaaggcg atccgaatga ttttgccgat atccccacgt tgcttagcgg tctggtagca
    24961 aaagtgcctg actcgcaggt gctggccgat ctcgatcatg tcgccagttg ggcttcccgc
    25021 aacggcggcg atgttcatcg tttaatgatc accggattct gctggggtgg acgtatcacc
    25081 tggctgtatg ccgcgcataa tccacagcta aaagccgcag tggcgtggta cggcaaactg
    25141 acgggcgaca agtcgctgaa ttcaccgaaa caacctgttg atatcgcaac cgatcttaac
    25201 gcgccggttc tcggcttata tggtggtcag gataacagca ttccgcagga gagcgttgaa
    25261 accatgcgcc aggcgctgcg ggctgctaat gcgaaagcag agattatcgt ctacccggat
    25321 gccgggcatg cattcaacgc cgattatcgc ccgagctatc atgccgaatc tgcgaaagat
    25381 ggctggcagc gtatgctgga gtggtttaag cagtatggtg ggaagcggaa ttagtttcct
    25441 cttagtctat atttgagatg gtgagtgaac gagtgcattt tgctgccatc tctgtccccc
    25501 aaaaaataat cagtatgaat tgattggaga gcacgactcc gctatttgac cttaagaaag
    25561 tcggcaagtc atagtcataa atgagtctct tcactggcag acaccttaga gatttaccat
    25621 caactcccat tctcaaacaa acattaatta ttatcaacac attcacatta attataaatt
    25681 atctcagacc ttcctgataa aaagatatta aagtgaaaca ttttgttttt cacattttag
    25741 ttcatccctt ttgggcgtaa ataaaggtaa aaaataacaa tactattttt ctttcacaaa
    25801 agaattaaca aaaggttttt caaggatgag tgatattatt tacctgacca taaccggaga
    25861 acaacaagga tgtatttcct ctcgttgcgg aacgagcgca tcaataggta atcgctggca
    25921 gattggccat gaagatgaaa tcttcgcgtt ctcgctatct aatagtatta cgaatacagg
    25981 caaagggtca caattacatg gccttagttt ctgtaagttg attgacaaaa gctccccgtt
    26041 acttattaac gctattaaca ataacgagca gttgttcatg gagtttgact tctatcggat
    26101 caatagattt ggtcgatggg agaaatatta caacattcaa ctccgcggcg cgctcttatc
    26161 agcaatcaat cacctgttta ctgagaacaa tctggataca gaaaccatca ctgtcagtta
    26221 tgaatatata ctctccaggc atctgattgc taatactgaa tttagtcatt tggcatttcc
    26281 cgataactac aaccgtctgt ttataccacg gcaaaaaact aaagatgata atggcctcaa
    26341 aacactcaat agtaaagcgg ttggcagact gcttgctgct ggcggcatat acaatgggaa
    26401 tatcgaagga ttcagggaaa cagcaaacaa gttgggagga gatgcacctg ctggttacga
    26461 tcaagttatg gataacaaag ggttactcat cgctggtgct tcaatagttg ctggagttgt
    26521 tctgggaaga atgagattcc ctgatttgga atcatttgag catttcggag ccagaggagc
    26581 agtatcagga agaccattta agcccgacct tgccggtggt ccaatagaaa acctgacgat
    26641 agatggcgta acaatcaata gggaaggtat cgctattgtt gaaaagcata tctccagatt
    26701 tgatcatgat ccagctaatg atgtaatgat ttcaaggcta aaaaaaatag caaataaaga
    26761 attgcttcct gaaaaatacg atctgaacta ctatactcat gaatgccgcg agtatcagcg
    26821 ttattgtaat ttaggatggg aaacaggtga accgaatggt ttagatggat atgaattatg
    26881 gaataatgca catactgcaa ctctagaaga ctttaaaatt aaagatactg acttatttca
    26941 tccggatgca aaaaaatgac gattacgata gttgaattta acgtgctgaa agttatggct
    27001 gagaaagata ttgactggag ctggatggtg ctggatcgta ctttagctat acgaaatatt
    27061 ccaggatttg gcaacgtagc gaatatcgta acaaagctgg ttaatcatgg gttggttgat
    27121 atcgtaaatg gtgagggcaa ctctaaacca cgctacagag tttctcaata tggattgaat
    27181 cttatcaaag aacaacaaga caacctcttc tgatccagcc attacaggag catgatttac
    27241 attgtgatct ttcaataaga tactcatcaa ttttggtaca gaatatcgtg atcactaaaa
    27301 atgaacttaa cgtgctgaac gttatgactg agaaagatat aaactggaac tggatgaatc
    27361 tcgatcgcac actatccata aggcaaatac caggattcgg taatgttgtt aatattgtta
    27421 acaaattagc taacgaaggt ttggttatta ttgaggatag cgggcataag tctatgcctc
    27481 actaccacgt tagtgaaaag ggatacaatc tcgtgcaacg tgagaataaa taacgtagcg
    27541 ttgattactg aacggaatca ctcaaaactg tgttttgcga aaaaaacgat tcggttattt
    27601 ctactcctga agatctgctt caatctggca ctcatgcaaa atggcttcca gtactggaag
    27661 ccattatcgt actgcaattt acaatgactc gttaatactc atcatccccg acgcaaattc
    27721 tgcgccgcct gcaccatgtt cgccagtgcc gcgcgggttt ctggccagcc gcgcgttttc
    27781 aggccacagt ccgggtttac ccacagacgc tctgccggaa tgcgttttgc cgctttcttc
    27841 agcagggctt caatccattc cacgctcggt acgtttggcg agtgaatgtc atagacgcct
    27901 ggaccaattt catttggata atcaaactct tcaaacgact ccagcaactc catgtcggaa
    27961 cgcgaggttt cgatggtgat gacgtctgcg tccagcgccg caatcgaatc catgatgtcg
    28021 ttgaactcgc agtaacacat gtgagtgtgg atttgtgtgt catctttcgc cacggcggcg
    28081 ttgatacgga aagcctccac gccccactgg agataggcat cccagtcgct gcgacgcagc
    28141 ggtaaacctt cgcgcagtgc cggttcatca atctggatga tgccaattcc agcggcttcc
    28201 aggtccgcca cttcatcacg cagcgccagc gcaatttgtt tggcgatggt ttcacggctg
    28261 acatcttcac gcgggaatga ccagcagagt atggttaccg gccccgtcag catccctttc
    28321 accggtttgt cggtcagcga ttgtgcatat ttcgcccact ccacggtaat tggtgccggg
    28381 cggctaacgt caccaatgac aatcggtggc ttcacgcagc gggaaccgta gctctgtacc
    28441 caaccgtttt gcgtaaagac aaagccatcc agatgctcgc caaagtattc caccatgtca
    28501 ttacgctcgg cctcgccatg taccagcaca tccagtccca aacgttcctg ctcgacaatg
    28561 gcctgcttga tatgttccgc aatgcccgtg cggtagttgt tggcgtcgag attacccttt
    28621 ttgaaatcca gacgcagtgt acgaatttcc gtggtttgcg ggaaggaacc aatcgtggta
    28681 gtcggccacg cgggcagttt aaaacgcgca cgctgggctt cagcacgcac ttcatagaca
    28741 ttcgcacgct gactgtcctg ggcggtaatg gctgcaagac gcttttctac cgccggatta
    28801 tggacacggg tagagtgacg acgcgcctga atcggagcgc tccattctgc cagagccgcc
    28861 gtatcaccac tgttcagcgc atcgcgcagc agtgccagtt catggcattt ttgtagggcg
    28921 aaggcaaacc agcttttcac ttctgcatca agacgcgttt ccacgctcag gtcgatgggg
    28981 ctgtgcagca acgagcagga agatgccacc cacaaatcgc gtttgccgac aatgtcctta
    29041 atttgcgcat atttctcggt aagatcggcg cgccagacgt tacgaccatt gatcagaccc
    29101 gcagacagta gccagtcaga aggcagacgc ttgtgcagtt cagcaacgtc atctttacca
    29161 tgtacaaggt ccacatgcag cccctgaaca ggcagcgcag taatcgtgtc gagatttggc
    29221 gttacgcctt caaaataggt agtcagcagc agtttcacct ggccctggag cgcgtcgtaa
    29281 gcgggtttgt atgcgtccag ccacgcctgc ggtagttcca gtaccagcgc tggttcgtcg
    29341 atctgtaccc actcaatgcc gcgttttgcc agttctgcca acacttgctg ataaaccggc
    29401 agaatgtcgt tcagcaggct caggcgatca aactgttcac ctttcacttt acccagccac
    29461 aggtaggtaa ccggccccag cagcacgggc ttgaccttgt ggcccagcgc cagcgcctcg
    29521 tccacttcct ccagcagctg cgtccaggtc agtttgaact gttggccttt aacgaactcc
    29581 ggcaccatgt agtgatagtt ggtgttaaac catttggtca tttccgctgc cgccgcaggt
    29641 tcgccagtcg gtgcacgtcc acgaccaata cggaacaggg tgtcgatatc taccgaacca
    29701 tctttgttct gatgacgtgg cggaacgtta cccagcagca gactggtggt cagtacatga
    29761 tcgtaccagg caaaatcgcc caccggcagc aggtcgatac ccgcctgctt ttgttgatcc
    29821 cagtgacggg cacgtaattc acgcccaaca gccagcagtt cttcacgcgt ggagttcccc
    29881 gcccaataac tctcttgcgc ttttttcagc tcgcgacgca ggccaacgcg agggaaaccg
    29941 agggtgtgat taagaattgt cattatatgc cccttatgga atttttagta tttagccgtc
    30001 cagatgttta cacatccata attggcggtt actgtatatt cctcaagcgc aaattattca
    30061 tgccgaagtg aaggactttc atgatcgaag taaaacacct gaaaacgcta caagcgttgc
    30121 ggaactgcgg ctcactcgca gccgctgcgg cgacgttgca tcaaacgcaa tccgccctgt
    30181 ctcaccagtt tagcgatctg gaacaacgcc ttggcttccg gctatttgtg cgtaaaagcc
    30241 agccgcttcg gtttacaccg cagggagaaa tcctgttgca actggcgaat caggtgctgc
    30301 ctcaaatcag ccaggcactg caagcctgca atgaaccgca gcagacgcgc ctgcgcattg
    30361 ccattgagtg ccatagctgt attcagtggc tgacgcccgc gttagaaaat ttccataaga
    30421 actggccgca ggtggagatg gattttaaat cgggcgtgac gtttgacccg cagcccgcgt
    30481 tgcagcaggg tgagctggat ctggtgatga cctccgatat tctgccgcgc agtggcctgc
    30541 attattcgcc gatgttcgat tatgaagtgc gtctggtgtt ggcacctgac catccactgg
    30601 cggcgaaaac gcgaatcacg ccggaagacc tcgccagcga aacgctgttg atttatccgg
    30661 tgcagcgtag ccgactggat gtctggcggc attttcttca gcccgcaggc gttagcccgt
    30721 cactgaaaag cgtcgataac accttgttgt tgattcagat ggttgccgcg cggatgggca
    30781 tcgctgcgct accgcattgg gtggtggaga gttttgaacg ccagggtctg gtggtcacca
    30841 aaaccctggg cgaaggcttg tggagccgac tgtacgccgc cgtgcgcgat ggcgagcagc
    30901 gtcagccgat tacagaagcg tttattcgtt cagcgcgcaa tcacgcctgc gatcatctgc
    30961 cgtttgtgaa gagcgcggag cgacccactt acgatgcacc cacagtgagg ccaggatcac
    31021 cagcgcgcct gtaataaacg ttggccagtg cggctgttgg tgccagatag ccaggttaac
    31081 caacagccct gccggaacgt gcatattatt cataatgccc agcgttccgg cgtccacctg
    31141 agtcgcgccg tagttccaca taaagtagcc aataccggaa gccaccacgc caagaaacac
    31201 cagaatgccc cattgcagcg tggtttgcgg cattttctgc gcatttccca gcaaaaacca
    31261 tgcaatcacc gccaccagga acgcaccaag atagaaccac gcaaaggcgt tatgctgtgg
    31321 catcggacga gtttccatca ggcgtttgta acccaccatg ccgatggcaa aagtgatatt
    31381 ggagagttgc accagcagca ggccagtcca gaagtggtcg gtgacctgat catagcgaat
    31441 aattccggca ccaatcaccg ccaacaaggc gctaaaagca tagccccagc gcagacggcg
    31501 cttactcatg atgtcataaa tcagcgtgat gtagagcgga gtcagtacgg tgaacagcaa
    31561 cagttcggag accgtcaggt agagataggc gcgaaaactc agcatataca tcacaccgag
    31621 ctgcatcgcg cccaccagca tatacaggcc aaccgttttc aggctattgc cacgggtacg
    31681 cagaaacggc agaaacacga gtgccgccag gccaacgcgc accagcactg caaaatagct
    31741 atcgacgtgc cccgcaaggt actcgccata aaagctaaag gagaaggccc acagaatcgt
    31801 ggtgatgata agtagcgcca caatggaaaa ctctttgatt aacgagtatc cattgtagcg
    31861 aagtatcagg ttgacaactg accaaataaa gaacgattac gataaataga gtttacgcag
    31921 ataatgcggc accgcgtctt cggcattagt accaatcact tccagctccg gatgcagatc
    31981 tttcagacgc tggtgcgcac tgcccataat gcagccttta cctgccatcg acagcatttc
    32041 ggcgtcgttc atcccgtcac caaaggcaat acaatctttc agactgtagc ccagtttctt
    32101 cgccaccgct tccagggcat ggccttttga aacgccaccc gccatcactt ccagacaagt
    32161 tagggtagag aagctgacgt tgacgcgatc gccccaacga gcgttgattg cctgctccag
    32221 cggcagcagt tgctcatgag aatcgcaggt gaagaacact ttgctgacgc cttccggctc
    32281 cagtaatcct ggctcataca gcgcatattt gaacactgct tctttaaaaa agcgcatctc
    32341 ttccgggcgg tggcgattca taaaccattc gtcgtcgcga taaacgttag taatgatgtc
    32401 cgggttgtcg ttgaccacgc caaacagatc gctggcaatg tcgcgatcca ggttatgagc
    32461 aaaaatcaga ttgccatcca gatcgtgaac acgcgcgcca ttggaggtaa tcatgtaaga
    32521 cttaatctcc agattatcgc gaatttgccc tacatcaacg tggtggcgac cggtcgcaaa
    32581 cacaaagtta atgccgcgcg cggtgagcag cttcagagtt tctttggcgt aaggggataa
    32641 cgtatggtcg ggagaaagta acgtgccatc taaatcagac gcaacaacct ggtacataag
    32701 aaatttaaac ctctgtagag cggtttccgc tgggtgagtt atgcctgttg aaaaaatcaa
    32761 caatggcgtg gagcgcgact gagcgcattg cgtccttttc aaaaaggatc tcatggtacg
    32821 cacctttaat taccagcggt cgtccccctt cgacaggatg gcccgcagcg gtgcggagtt
    32881 cacaaaaacg gtcgtgcatg cggttatcca ccacgcgttc ctcttcagcc tgcaagagaa
    32941 gcgttggcgt ggcgtcatca cccgcaccgg ctaacacctg ttcgccagcc agaatacttt
    33001 cgcgtaccca atggtaggtc ggcccaccga cgcgaatcgt tgggtcatca gcataaaagc
    33061 gtaagttacg tcgatatcgc tgtctgctgt gggtcagtac gttgatagca aacggcaacg
    33121 cgcgccagcg cccggtgcct attgcatagc catcacggaa acgtggatgc gcttcggccc
    33181 agttaaggat ctgccgcgcc ataaatgacg gcatacgaat cacgatccca aacattggcg
    33241 cagttagcgc aatggcgtca catacacctg gatggcgttg cagaaataac gtggagatcg
    33301 caccgcccat cgaatgtgcc agtatatagc gcttacgcca cggaccaggc tggacctcct
    33361 gttgccagaa tgccgccagg tcatcgacat aatcattaaa gcgattaaca tgcccaagat
    33421 gcggatcggc taacaggcga ccggaacgtc cctgcccgcg atggtcgatg attaagacat
    33481 caaaccccaa atggaacagg tcgtaggcca gttccgcata ttttacgtag ctctcaatac
    33541 gccccgggca gatgactatc acccggtcat ggtgctgtgc gcgaaaacgg acaaagcgca
    33601 ccggaatgtc atctacacca gtaaactctg cttcatcacg ctgacgccag aaatcagtca
    33661 gcggtcccat ggtaaaagca gcaaacgcgt tttctcttgt ttcccagtct ttttgctgct
    33721 gaaacatcgg gtaatctgcc tcttaaacca cgtaaaatcg tttttttaag cgtgcctgac
    33781 acaacgcttc gacagtagcg tattgtggca caaaaataga cacaccggga gttcatcatg
    33841 accttagaat ggtggtttgc ctacctgctg acatcgatca ttttaagcct gtcgccaggc
    33901 tctggtgcaa tcaacaccat gaccacctcg ctcaaccacg gttatcgcgg cgcggtggcg
    33961 tctattgctg ggcttcagac cggactggcg attcatattg tgctggttgg cgtggggttg
    34021 gggacgctat tttcccgctc agtgattgcg tttgaagtgt tgaagtgggc aggcgcagct
    34081 tacttgattt ggcttgggat ccagcagtgg cgcgccgctg gcgcaattga tcttaaaccg
    34141 ctggcctcta ctcaatcgcg tcgacatttg ttccagcgcg cagtttttgt caatctcacc
    34201 aatcccaaaa gtattgtgtt tctggcggcg ctatttccgc aattcatcat gccgcagcag
    34261 ccacaactga tgcagtatat cgtgctcggc gtcaccacta ttgtggtcga tattattgtg
    34321 atgatcggtt acgccacact tgctcaacgg attgcgttat ggataaaagg gccgaaacag
    34381 atgaaggcat tgaataagat ttttggctcg ctgtttatgc tggtaggagc gttgttggca
    34441 tcggcgaggc atgcgtgaaa aataatgtcg gatgcggcat aaacgcctta tccgacttac
    34501 tctgatgaag cgtttggcat caacgagaaa taatcaaatg aatgccaaat ccggcaaata
    34561 acgccccggc aaaaccatca atccacttcg ccagacgttg ataaccacgg cgcatttgcg
    34621 gtagagcaaa caggctggca accaccgtaa accatgccag tgtttcgata ataatcaacg
    34681 cgaaaatgcc ccagcgctca gtggtgccga cgttatcgcc gacaaacagc gagaataccg
    34741 aaccaaagta gataattgct ttcggattgg cgagattggt cagtaagcct ttcaggaaac
    34801 tgcgcccgct ttttgccagt tctacctgtg gcgcaggcgc agaaacgacc tcttttttca
    34861 gtgcgctacg cagcatctga taacccatcc agcagagata caggccaccg cccaccataa
    34921 tcagcgtatg cagccaggcc attttttcga taatcaaatg caggccaagc aacgcgatcc
    34981 ctgcccagac cattacgccg caggtaatgc ccagcacgcc catcatcgcc tctttacggg
    35041 aacgactgac agcggtctga gaaacaaaga agaaatcggg accggggctc ataagcgcca
    35101 caatgtgcac catggcaacg gtgaggaata acatcaacat tcaattgact cgcggagaaa
    35161 tcgtttacgg agtcttcatc ctggcacttt tttatgctgc tgactactct tcgtcatcgc
    35221 catcgacatg cgcacgaatt agcgccataa acggtttgcc aaagcgttcc agcttgcgca
    35281 tcccaacgcc gttaacgctg agcatttcgc tggcggagat cggcatctgt tccgccatct
    35341 caatcaaggt cgcatcgtta aacaccacat acggcgggac attactttca tcggcaatcg
    35401 atttacgcag tttgcgtaat ttggcgaaca gtttgcgatc gtaattgcca ccgaaagatt
    35461 tctgcattgc tttcggtttg agcgccacaa tacgcggcac ggcaagttgt aaagaggatt
    35521 cgccgcgcaa caccgggcgt gcggcttctg tcagttgtaa tgccgaatgc tgggcgatgt
    35581 tttgcgtcac cagaccgaga tgaatcaact ggcggattac gctcacccag tgctcatgac
    35641 ttttatcgcg gcccatgccg tagactttca gtttgtcatg accatagtcg cggattcgct
    35701 ggttattggc gccgcgaatc acttccacta catagcccat cccaaaacgc tgattcacac
    35761 gaccaatggt ggaaagcgca atctgcgcat cggttgaacc atcgtattgt ttcggcggat
    35821 cgaggcagat atcgcagttc ccgcacggct cctgccgccc ttcgccaaag tagttcagca
    35881 gcaccagacg acggcaagtt tgcgcttcgg caaaagcgcc catcgcattg agtttatggc
    35941 gctcgatatc ctgcaactgc ccctgcggct tctcttccag acaacggcgc agccacgcca
    36001 tatcagccgg atcgtaaaac agcatcgctt ccgcgggcag gccatcacgc ccggcgcgac
    36061 cggtttcctg ataataggat tcgatattgc gtggaatatc aaagtgaacc acgaagcgga
    36121 cgttaggctt gttgatgccc atgccgaacg ctaccgtcgc caccacaatt tgcaggtcat
    36181 cacgctggaa tttttcctgc acgtccgcac ggacatcgtt ttcaagcccg gcgtgataag
    36241 ccgctgcgct gatacccttg ctttgcagac gcgcggcggt gtcttctact ttcgcacgac
    36301 tattacagta aataatcccc gacttaccgc gttgttcctg tacataacgc atcaactgat
    36361 cgagcggttt gaatttctcc atcagcatgt agcgaatatt cggacggtca aaactgctaa
    36421 tttgaatcag cggatcgttc agccccagca ggcgcacgat atcctgccgc gtggtgtcgt
    36481 cggcggtggc ggttagcgcc ataaacggca gcgtcgggaa ccgctggcgc aactgaccga
    36541 gcgcggcata ttccgggcgg aaatcgtggc cccattggga gatacagtgc gcttcatcaa
    36601 cggctaataa caccggattc cagtgcgcca gatgctcaag aaagttatcc agcatcaggc
    36661 gttccggggc gatataaagc agacgaattt gcccggtgcg acagcctgtc atcacttcaa
    36721 gctgctgctc gcgggtttgc gtcgagttaa ggcacgccgc cgccacaccg ttggcctgta
    36781 gctgatccac ctgatctttc atcaacgaaa tcagtggtga aacaaccacg gtaagaccgt
    36841 ttagcagtaa ggcaggaatt tgatagcaaa gggattttcc gccaccagtg ggcatgacga
    36901 ccaggcaatc gcgaccggaa agtacggtgt cgataatctc ttcctggccg gggcgaaact
    36961 gttggtagcc aaaggtttct tgtaaaacct gtttagctcc ggactccaga ttcaacactt
    37021 ccgcctgcgc cacattaacc ccgattgcct gaaataaaaa caggcgctat tttcagcgcc
    37081 tgcgcgagaa actgcaatgc ttttagtctc tgagagggta atcctggata ctggattcca
    37141 gttttgcctt tcaatcaact cacattagaa caagtcgttc agcataaccc ccacacccac
    37201 acgggtctgg ttaaagttat agtcgatgag cgattcgcca taaccgctgt aaacctgggt
    37261 ataaaggcgc acatgcctgg tgatcgggta acttaagcct aactccgcac caccgtaacc
    37321 ggtgttccag ttgtactgtc ctttcgcact cagcaccgca tcacccaggt gatagccgat
    37381 tttaagctgg tagtaaccca tatatttggt gatatctggg ttatcgtcag tattccccac
    37441 cacataccac ggcttcacct ctaccagcca gttaccgttt tctgccatca gacgggtata
    37501 aaggcggttc cagctgcggg aagtcgggtc tgaacgcccg ttagagtcgt ggttataacc
    37561 catctccaca tcgcgcagcg tccagccagc aaagcggtaa tcggtggcaa aaccaaggaa
    37621 caattgcggt tcgtagttgg tttcacgaaa cggtgaagac tcttcgctat tggacagttg
    37681 ccaccaggat ttttgcgtgt aagaagcccc caacaccgag tttggcccca atattccgcg
    37741 ccacagcgga aatgccaggc tcaactggaa tttcacttca tccttacgcg cgttttccgc
    37801 ccagtcataa ctggcgatcg cttctttgtt cagatcgctg gtttgggtgt agatgaggta
    37861 gttggtgtca taaggataga gcgtgaaagg attgtcatgc tcctgcagca tattcgcaat
    37921 aatactgcca cgcaccgcag gcgcgtcatg cacctctttt accgttgcct cttgcgcata
    37981 cactgccatt ggcaacataa acaccggcaa caaccagccc tgcagagtcc gcatcggtcg
    38041 tgttctccgt aacgaattat tgattagatg aatatttttc tgcgggctat tttacatact
    38101 taactccttt gccgttagtt atcccttcct aaagtagaaa accacaatta agaagcataa
    38161 aatcaacatt tcattaacca atgaggtgta aggatgatat gtctgccgta ctgaccgctg
    38221 aacaagccct gaaattagtg ggtgagatgt ttgtttatca catgccattt aaccgcgcat
    38281 tggggatgga actggagcgt tacgaaaaag agttcgcaca gctggccttt aaaaatcagc
    38341 caatgatggt gggtaactgg gcgcaaagca ttttgcacgg cggggtcatt gcgtcggcgc
    38401 tggatgtcgc cgcaggtctg gtgtgcgtgg gaagtacctt aacccgccac gaaaccatca
    38461 gtgaagatga actacgccag cggctatcgc ggatggggac cattgatctt cgcgtcgatt
    38521 atctgcgccc aggcaggggc gagcgtttta ctgctaccag tagtctgttg cgtgcaggca
    38581 ataaagtcgc cgttgcccgc gttgaattac acaatgaaga acagctttat atagccagtg
    38641 ccaccgccac ctatatggta ggttgataac gtaaaagcag gtaaactgct tttaccaata
    38701 aaaaatgagt tttgccaatg gacaccaaac aaacgcggca gggcgtatta ctcgctcttg
    38761 ccgcttattt tatttggggt attgcgccag cgtacttcaa gctgatttac tacgtgcccg
    38821 ccgatgagat cctgacgcat cgcgtgatct ggtcgttttt ctttatggtg gtgctgatga
    38881 gcatttgccg ccagtggtcc tatttaaaaa cgctgattca gacaccaaag aaaatcttca
    38941 tgctggcagt ctctgccgtg ctgattggtg gcaactggct actgtttatc tgggcggtga
    39001 acaatcacca tatgctggaa gcgagccttg gttactttat taacccgctg gtgaacattg
    39061 tgctggggat gattttcctc ggcgagcgat tccgccggat gcaatggctg gcggtgattc
    39121 tggcgatatg tggtgtattg gtccagctct ggacttttgg ttcgctgcca atcatcgcgc
    39181 tggggctggc gtttagtttt gccttctacg gcctggtacg caagaaaatt gccgttgaag
    39241 cgcaaaccgg catgttaatc gaaaccatgt ggctgctgcc cgtggcggcg atttacctgt
    39301 ttgctattgc cgacagctca accagccata tggggcaaaa cccgatgtcg ctgaatttac
    39361 tgctgatcgc cgccggtatt gtcaccaccg tgccactgtt atgttttacc gcagcagcta
    39421 cacgcttacg actctcaaca ttaggctttt tccagtacat tgggccgacg ctaatgttcc
    39481 tgttggcagt gacgttttat ggtgaaaaac cgggtgccga taagatggtg actttcacct
    39541 ttatttgggt ggcgctggca atttttgtga tggatgcgat ttatacgcag cgtagaacgg
    39601 ccaaataaaa aataaatata ttctgaaaaa atatgccgac tcttgcatga gagtgggcat
    39661 attgccatgc tgacaggaga ctcacataag gaaaggcaga atttccagac gtcgttgtat
    39721 attttcattt gctttaataa atttcatttt tggtaaattc agcattatg
//
LOCUS       DAZGQL010000041        39712 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.041, whole genome shotgun sequence.
ACCESSION   DAZGQL010000041 DAZGQL010000000
VERSION     DAZGQL010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39712
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.041"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            75..1835
                     /gene="tssF"
                     /locus_tag="ACHKIR_002381"
     CDS             75..1835
                     /gene="tssF"
                     /locus_tag="ACHKIR_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFO9990114.1"
                     /translation="MDDLTQRYYEAEMRYLREAGKEFAQAYPDRAAMLNLDKPGARDP
                     YVERLFEGFAFLMGRLREKLDDDLPELTEGLVSLLWPHYLRTIPSLSVVELSTDHRQM
                     KQSETLSNFQVLSRPVGERRTRCVYSATRDITLHPLALPDVSLQYEPDGRSVIRLRFE
                     CGPLVGDWSQIDLSRLPLYLNADSPVACALHRALTLGTQQFWLRLPGQERRVLDAHFS
                     PMGFDDNDRLWPKGESAFSGYQLLLEYFTFREKFMFVALNGLENVIWPEGMSGFEIDV
                     VLSENWPHDLPFDSDNIRLHCVPVINLFPLEADPLHLSPLENEFLLRPMRIQDGHTEI
                     YSVDNIISSRHTGSQAYVPFSSFRHRGGMLRHDAPERYYHTRVKRGPSGLHDTWLILG
                     GDAFDSDRMLEDETLSLSLTGTNGQLPRKALQSTLLDTPVHASQNVLRVRNLCAPTQP
                     CYPPARDRFHWRVLSHLGSNFLSMMDNAEILRGTLALYDWTESEMNRRRLSAIVDVQH
                     SLIQRFERGFLLRGVDIQVTLDSTGFAGEGDITLFGELLHRFFALYADIHLFTQLTLI
                     LQPTGKCLQWTEHHSQRVPG"
     gene            1799..2878
                     /gene="tssG"
                     /locus_tag="ACHKIR_002382"
     CDS             1799..2878
                     /gene="tssG"
                     /locus_tag="ACHKIR_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000373314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFO9990115.1"
                     /translation="MDRASQPARTGLTDRLAPDIARINFYRFCQLLEQSQQNAPLGST
                     DSPATDAVRFRPHPGMGFPVSELKNVERDVGNPDAPPTVRTTFLGLYGVDSPLPTAYL
                     DYITQRHDGHDAVMAFLDIFNHRFITQYYRIWRKYNYPASFEAGAVDDISRCLLGLIG
                     LGIPGSENHIATPVSRFLALLSVMRLPTRTAEGVTALVGLLAPLTKATVVPHDPQPVI
                     LPAPAGLSKNSRISLKTRTLLGRTGTDVNSQLLLKLYTEDAAEARGWLPGGQLHSDLL
                     VLLRVYLGWRCQARLQLTLPVSLLPAARLGKQRVQISRTGILRASFAAPATGTVTVSL
                     GRYQGLIPAFSIRNRESMTHVSYSF"
     gene            2859..3395
                     /gene="tssJ"
                     /locus_tag="ACHKIR_002383"
     CDS             2859..3395
                     /gene="tssJ"
                     /locus_tag="ACHKIR_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFO9990116.1"
                     /translation="MFPIRFKRPALLCMAMLTVVLSGCGLIQKVVDESKSVASAVFYK
                     QIKILHLDFFSRSALNTDAEDTPLSTMVHVWQLKTREDFDKADYDTLFMQEEKTLEKD
                     VLAKHTVWVKPEGTASLNVPLDKETQFVAIIGQFYHPDEKSDSWRLVIKRDELEADKP
                     RSIELMRSDLRLLPLKDK"
     gene            3399..3827
                     /gene="tssE"
                     /locus_tag="ACHKIR_002384"
     CDS             3399..3827
                     /gene="tssE"
                     /locus_tag="ACHKIR_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000106970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFO9990117.1"
                     /translation="MATPSLYEMLTFSFSGELPLEQVSERDQLILSVMDNMQRIINCR
                     AGTLAHLPDYGLPDLSLIHQGMAAGIHSLMRQIEETLLRYEPRLNQIQVELLPQPGPG
                     HLNYLIHAQLPDTGWIRFDGVFSPEGRIVLRHLKQQERAY"
     gene            3827..5203
                     /locus_tag="ACHKIR_002385"
     CDS             3827..5203
                     /locus_tag="ACHKIR_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
                     /protein_id="HFO9990118.1"
                     /translation="MASNANFISQFVMGGDPCTYKESGELQAEMSKLTHPARPDVDWR
                     QVEKLCLALFRQNGVELQTLVCYVLAITRRQGLAGMADGLGSLDILLQRWADFWPVQV
                     HSRISLLSWVTEKMQQALRTLDIQYQDLPQIYRCVQHLSAIETTLQQCELWHMTKLDV
                     LSGQFRNAALRLERLAPQGAETIITPPELPRREMNQPKKSEESPQPVFATRSVQQNDK
                     DASPPVPSPEISRQRTWPIFMAGMVVMAGLGGTGLWGWSQLNQPDALIQRIQLSVMPL
                     PQSLESGELAKLDVKDKALLAQDRTIAASQMQLEQLNKLPARWPLEQGYRQLRQLDAL
                     WPDNPQVRALNAQWRKQRELSALSAEALNGYAQAQSQLQRLSAQLDALDERKGRYLTG
                     SELKTAVYGIRQSLKEPPLEELLRQLEEQKQTGEVSPTLLTQIDTRLNQLLNRYVILL
                     DTKVEQSQ"
     gene            complement(5505..6452)
                     /locus_tag="ACHKIR_002386"
     CDS             complement(5505..6452)
                     /locus_tag="ACHKIR_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001361319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFO9990119.1"
                     /translation="MKNVLVTVPSFSARCVSASKLLRENHFNLIIKNNVEHLVKSEST
                     VLRESICAVIAGKDCYQADTLSRLPGVKIISRFGTGIDNIDLHTARQSGIVVNNAVGI
                     NSNAVAEFIIGLIFASMRNIPGSYHAMQNGYWGESHGCELQGKRIGLLGYGNIGKTLA
                     KRLSGFDVELLAFDKQPDYQVADKAGVQFVSIEDIFMQSHVIIVLLPFSSELENFISH
                     KYLSMMRNGALIINAARGKLLDEGALLQVIEERNVFAALDVFSSEPLAQFSPLLHAKN
                     IITTPHIAAATVESYQQTGIHVAQSIIDYFAGREIKNVL"
     gene            complement(6523..7119)
                     /locus_tag="ACHKIR_002387"
     CDS             complement(6523..7119)
                     /locus_tag="ACHKIR_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFO9990120.1"
                     /translation="MNSTDLIHLIKHFMRNEHKAVEEVIDSPLSEVANLIKVLQSCQG
                     KVVFIGVGKSGIIARKLAATFASTGTPSFFVHGTEAVHGDLGMVAKDDVVILISNSGE
                     TTEILATLPSLKKMGNYLISFTRSHRSSLAISCDLSVEIPVKSEADNLGLAPSCSSTV
                     VLVVGDAVALALSELKKFTRADFGLYHPGGALGIKANS"
     gene            complement(7122..8297)
                     /locus_tag="ACHKIR_002388"
     CDS             complement(7122..8297)
                     /locus_tag="ACHKIR_002388"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFO9990121.1"
                     /translation="MDIFNTPISRKGTYCTQWDFCEDRFGVKDVLPFSISDMDLPIPD
                     AITRALKKRLEHPILGYSRWQHGEYLNAIVNWYHQQYQTDIKPEWITYSPSVMYSIAK
                     AIELLTSHGDNILVFTPVYNAFFDVIKHSERNILTASLIIKNEGGYAINWQDFDLKIK
                     SAKMVLLCNPHNPTGTVWSEEELLKIATSCTRHNVWLCSDEIHSDFVFNRSFTSALKI
                     KKEKVVVFNSISKTFNVPALTGSYLISTDEDYNHKFRTISRYRDFVNSPSVLNIIATI
                     VAYNECEGWLKSLKAHIASNIQFTQQYLNENTPELIVRPADGCYFSWIDCSAIGYSFD
                     EFYSRLIHEGKVGIMAGNVYGTEGEGYLRLNLACGREKLYMGLTRLASVIKNINQGK"
     gene            complement(8297..9877)
                     /locus_tag="ACHKIR_002389"
     CDS             complement(8297..9877)
                     /locus_tag="ACHKIR_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000810566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFO9990122.1"
                     /translation="MKQKKAWSFFQSLGKAFMYPIALLSVCGMMLGLGSGLASDDMAK
                     LIPFLAIPIIKTILDFIVSLGLFAFVNLPVLFAIAIPLGLLKDKEDKAYGAFSGLIGF
                     MAMHLGTNFYLKQHDLLVVADQMSTHGQNIILGIQSYNTSVLGGIVAGLLVASMYKKI
                     VNLRIPESLGFYSGPRLVPIITLIVMSGFGLIIPFIWPPFFNLFMLIGHWISTSGPVG
                     YFFYAVAERVTIPFGLNHLVTSVFRFTPIGGSAVIGGEEYYGTLNMFMAYVKENAVIP
                     LDLAGKMEQGKLMIQYGLAGAALAMYRTAHAQNRKAIKALLISGVLTVIIGGVSEPIE
                     FLFLFVSPLLFVFHAFMNGFANMVLPYMGVKMGFTGDLIQFISFGVLRGTRTGWPIAV
                     CVEVAYFFIYYFVFRWTILKFNLMTVGREESSPVTLNAHEDTAIADTPTLDKSELQAA
                     EQMVKALGGKENIKSLDNCVTRLRLTIADMGLIDEVAIKRAGGIAVVKLDQNTLQVII
                     GTKVIALRRDMDNYMGMR"
     gene            complement(9909..10733)
                     /locus_tag="ACHKIR_002390"
     CDS             complement(9909..10733)
                     /locus_tag="ACHKIR_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFO9990123.1"
                     /translation="MIIEKVMNNNCVQASMNGQEVIISGPGVGYNKKYGMSVPEHPAN
                     RIFYVRNEQKNKLYKLIEHVDVEYIFVAEKIVQYAEKNLEKNLNPSLLLILADHISNA
                     ISRVVSGIQINNVFLDEIKALYKAEYAISRDALTIINEQFSVQLPDDEIGFIALHILN
                     NYENSVDYESVRIIELSQIITELIEVVYNRKVDRSSFNYSRFMMHLKYFSSRVLCNEK
                     IKQKDIGDIYEQFLEKDILLQRAIHEIERYLYATFKYELILEEKLYLSIRTKVLMD"
     gene            complement(10991..12244)
                     /gene="amiC"
                     /locus_tag="ACHKIR_002391"
     CDS             complement(10991..12244)
                     /gene="amiC"
                     /locus_tag="ACHKIR_002391"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Like AmiA, dependent on TAT (Twin-Arginine
                     Translocation) system for export; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HFO9990124.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            12476..13807
                     /gene="argA"
                     /locus_tag="ACHKIR_002392"
     CDS             12476..13807
                     /gene="argA"
                     /locus_tag="ACHKIR_002392"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HFO9990125.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(14045..15871)
                     /gene="recD"
                     /locus_tag="ACHKIR_002393"
     CDS             complement(14045..15871)
                     /gene="recD"
                     /locus_tag="ACHKIR_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HFO9990126.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERAGQLSRLTG
                     THVPSGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRNPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(15871..19413)
                     /gene="recB"
                     /locus_tag="ACHKIR_002394"
     CDS             complement(15871..19413)
                     /gene="recB"
                     /locus_tag="ACHKIR_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HFO9990127.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYKLLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDAVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIDKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALMLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEAERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDAITAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVDEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDSLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYERHFGGVIYLFLRGV
                     DKEHPQQGIYATRPNAGLIDLMDEMFAGITLEEA"
     gene            complement(19406..22294)
                     /gene="ptrA"
                     /locus_tag="ACHKIR_002395"
     CDS             complement(19406..22294)
                     /gene="ptrA"
                     /locus_tag="ACHKIR_002395"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HFO9990128.1"
                     /translation="MPRSTWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFSSEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPEEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(22470..25838)
                     /gene="recC"
                     /locus_tag="ACHKIR_002396"
     CDS             complement(22470..25838)
                     /gene="recC"
                     /locus_tag="ACHKIR_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HFO9990129.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWEIGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDADRLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIARRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(25851..26174)
                     /gene="ppdC"
                     /locus_tag="ACHKIR_002397"
     CDS             complement(25851..26174)
                     /gene="ppdC"
                     /locus_tag="ACHKIR_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HFO9990130.1"
                     /translation="MSASLRNQQGFSLPEVMLAMVLMVMIVTALSGFQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(26159..26566)
                     /gene="ygdB"
                     /locus_tag="ACHKIR_002398"
     CDS             complement(26159..26566)
                     /gene="ygdB"
                     /locus_tag="ACHKIR_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HFO9990131.1"
                     /translation="MNREKGVSSLALVLMLLILGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALEWGKMHSWQTLPAVQCLLYAATGARVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGNIVFSPRGWSDFCPLKERALCQLP"
     gene            complement(26563..27126)
                     /gene="ppdB"
                     /locus_tag="ACHKIR_002399"
     CDS             complement(26563..27126)
                     /gene="ppdB"
                     /locus_tag="ACHKIR_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFO9990132.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTNTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCVIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMHAASKADPQTVVDASYSVTGSNL"
     gene            complement(27117..27587)
                     /gene="ppdA"
                     /locus_tag="ACHKIR_002400"
     CDS             complement(27117..27587)
                     /gene="ppdA"
                     /locus_tag="ACHKIR_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFO9990133.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISLIREGTLWCLVSSVAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(27772..28566)
                     /gene="thyA"
                     /locus_tag="ACHKIR_002401"
     CDS             complement(27772..28566)
                     /gene="thyA"
                     /locus_tag="ACHKIR_002401"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFO9990134.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(28573..29448)
                     /gene="lgt"
                     /locus_tag="ACHKIR_002402"
     CDS             complement(28573..29448)
                     /gene="lgt"
                     /locus_tag="ACHKIR_002402"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFO9990135.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(29599..31845)
                     /gene="ptsP"
                     /locus_tag="ACHKIR_002403"
     CDS             complement(29599..31845)
                     /gene="ptsP"
                     /locus_tag="ACHKIR_002403"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFO9990136.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(31858..32388)
                     /gene="rppH"
                     /locus_tag="ACHKIR_002404"
     CDS             complement(31858..32388)
                     /gene="rppH"
                     /locus_tag="ACHKIR_002404"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HFO9990137.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            complement(32741..32887)
                     /gene="ygdT"
                     /locus_tag="ACHKIR_002405"
     CDS             complement(32741..32887)
                     /gene="ygdT"
                     /locus_tag="ACHKIR_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgdT"
                     /protein_id="HFO9990138.1"
                     /translation="MLSTESWDNCEKPSLLFPFTALTCDETPVFSGSVLNLVAHSVDK
                     YGIG"
     gene            33073..33762
                     /gene="mutH"
                     /locus_tag="ACHKIR_002406"
     CDS             33073..33762
                     /gene="mutH"
                     /locus_tag="ACHKIR_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HFO9990139.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAKRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQIERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            33831..34544
                     /gene="ygdQ"
                     /locus_tag="ACHKIR_002407"
     CDS             33831..34544
                     /gene="ygdQ"
                     /locus_tag="ACHKIR_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFO9990140.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            34682..34900
                     /gene="ygdR"
                     /locus_tag="ACHKIR_002408"
     CDS             34682..34900
                     /gene="ygdR"
                     /locus_tag="ACHKIR_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HFO9990141.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            35008..36048
                     /gene="tas"
                     /locus_tag="ACHKIR_002409"
     CDS             35008..36048
                     /gene="tas"
                     /locus_tag="ACHKIR_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HFO9990142.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMEQLKTNVESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(36080..37273)
                     /gene="lplT"
                     /locus_tag="ACHKIR_002410"
     CDS             complement(36080..37273)
                     /gene="lplT"
                     /locus_tag="ACHKIR_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HFO9990143.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLISMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLSAYALLMLIGVLGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRY"
     gene            complement(37266..39425)
                     /gene="aas"
                     /locus_tag="ACHKIR_002411"
     CDS             complement(37266..39425)
                     /gene="aas"
                     /locus_tag="ACHKIR_002411"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008779 -
                     acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008922 - long-chain fatty acid
                     [acyl-carrier-protein] ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HFO9990144.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LALFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVEMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
BASE COUNT         9905 a        10586 c         9735 g         9486 t
ORIGIN      
        1 aataaattta actatgatgt tacttctaca cctgcatgat attcactttc aatgaaagga
       61 agataatccc tccgatggac gatctaaccc aacgctacta cgaagccgaa atgcgctacc
      121 tgcgtgaagc ggggaaagaa tttgcgcagg cttaccccga ccgcgccgcg atgctcaatc
      181 ttgataaacc cggcgcacgc gatccctatg tggagcgcct gttcgaaggc ttcgcttttt
      241 taatgggtcg cctgcgtgaa aagctggatg acgacctgcc cgaactgacc gaggggctgg
      301 tcagcctgct gtggcctcac tacctgcgaa ccatcccgtc actttccgtg gttgaactgt
      361 cgacagatca ccggcagatg aagcagagcg aaacactgag taattttcag gtattgtcgc
      421 gtcctgttgg cgagcgcaga acccgctgtg tctacagtgc tacgcgggat atcacgctgc
      481 atccgctggc gctgccggac gtgtcattgc aatatgaacc ggacgggcgc tcggttatcc
      541 gtctgcgctt tgaatgcggc ccgctggttg gtgactggtc acagatcgat ctatcccgtt
      601 tgccgctcta tctcaatgcc gacagcccgg tggcctgtgc gctgcaccgg gctttaacgc
      661 ttggcaccca gcagttctgg ctgcgtctgc cggggcagga acgccgtgtg ctggacgcgc
      721 acttttcccc gatgggtttt gatgacaatg accgactgtg gccaaaaggc gagtcggcgt
      781 tcagcggcta ccagttgctg ctggagtatt tcaccttccg cgaaaagttt atgtttgtgg
      841 cgctgaacgg gctggagaac gtcatctggc cggaagggat gagcggcttt gagattgacg
      901 tggtgctgag cgagaactgg cctcacgacc tgccgtttga cagcgataat atccgtctgc
      961 actgcgtgcc ggtcatcaac ctgttcccgc tggaagccga tccgttacac ctttcgccac
     1021 tggaaaacga atttctgctg cgcccgatgc gtattcagga cgggcatacc gagatttatt
     1081 ccgtcgacaa cattatttct tcgcgccata ccggcagtca ggcgtatgtc ccgttttcca
     1141 gctttcgtca ccggggcggg atgctgcgcc acgatgcacc ggaacgctat taccatacgc
     1201 gggttaagcg cggcccttcc ggcctgcatg atacctggct gattctgggc ggcgatgcgt
     1261 ttgattccga ccggatgctg gaggatgaaa cgctctcttt aagcctgacc ggcactaacg
     1321 gacagcttcc ccgtaaggct ttacagagca cgctgcttga tacgccagtt catgcctcac
     1381 agaacgtcct gcgggtgcgc aacctgtgcg ccccgacgca gccctgttat cccccggcgc
     1441 gtgaccgctt ccactggcgg gtgctcagcc acctcggttc taactttctt tcaatgatgg
     1501 ataacgccga aattctgcgc ggcacgctgg cgctgtacga ctggaccgaa agcgagatga
     1561 accgccgccg tctgtcggcg attgtcgacg ttcagcacag cctgatacag cgttttgaaa
     1621 gaggctttct gctgcgtggc gtggatattc aggtgacgct ggacagcacc ggctttgccg
     1681 gagagggtga tatcacgctg tttggcgaac tgctgcaccg cttctttgca ctgtatgccg
     1741 atatccactt attcactcaa ctgaccttaa ttctgcaacc cacaggaaag tgcctgcaat
     1801 ggacagagca tcacagccag cgcgtaccgg gctgaccgac cggctggccc cggatattgc
     1861 gcggatcaat ttttaccgtt tttgccagtt gctggaacag agccagcaga acgcgccgct
     1921 gggcagtacc gacagccccg ccaccgatgc ggtgcgcttt cgtcctcatc caggtatggg
     1981 ctttccggtc agcgagttaa aaaacgttga acgtgacgtg ggcaatcccg acgcgccccc
     2041 cacggtgcgc accacttttc tcggcctgta cggcgtggat tcgccgctgc caaccgccta
     2101 tctggactac attacccagc gtcatgacgg acatgacgca gtgatggcgt ttctcgatat
     2161 ttttaaccat cgctttatta ctcagtacta ccgcatctgg cgcaaatata actacccggc
     2221 gtcgtttgaa gcaggtgccg tggatgacat ttcccgttgt ctgctgggac tcatcgggct
     2281 gggtattccc ggcagcgaga accatatcgc cacgccggta tcccgttttc tggccctgct
     2341 cagcgtgatg cgcctgccga cgcgcacggc ggaaggcgtc acggcactgg tagggctgct
     2401 tgccccgctg accaaagcga cggtggtgcc tcacgatccg cagcctgtaa ttctcccggc
     2461 ccctgcgggt ctgtcaaaaa attcacgcat cagcctgaaa acgcgaacgc tgctggggcg
     2521 taccggtacc gacgtcaaca gccagttgct gctgaaactg tataccgaag atgccgcaga
     2581 agcacgtggc tggctgcccg gcgggcaact gcacagcgat ttgctggtgc tgctgcgcgt
     2641 ttatctgggc tggcgctgtc aggcccgtct gcaactgacg ctgcccgtca gcctgttgcc
     2701 tgctgcccgg ctgggaaaac agcgtgtaca gattagccgt accggcatcc ttcgcgcctc
     2761 ctttgccgcg cccgcgaccg gaacagtgac ggtcagtctg gggcgttatc agggattaat
     2821 acccgctttt tccattcgta acagggagag tatgactcat gtttcctatt cgttttaaac
     2881 gtccggcgtt gctctgtatg gcgatgctga cggttgttct gagtggctgc gggctgattc
     2941 agaaagtggt ggatgaatcg aaaagcgtgg cctcagccgt tttctacaaa caaatcaaaa
     3001 tactgcatct cgatttcttc tcccgcagcg ccctgaatac ggatgcggaa gatacgccgc
     3061 tttccacgat ggtgcatgtc tggcaactga aaacccgcga agattttgat aaggcggatt
     3121 atgacaccct gtttatgcag gaagagaaga cgctggagaa ggacgtactg gcaaaacaca
     3181 ccgtctgggt aaaacctgaa ggcacggcat ccctgaatgt gccgctggat aaagagacgc
     3241 agtttgtcgc cattattggg cagttttatc acccggatga aaaaagcgac agctggcgtc
     3301 tggtgatcaa aagggacgaa ctggaggccg acaagccgcg ctcgattgaa ctgatgagaa
     3361 gcgacctgcg actgctgcct ctcaaggata aataaccgat ggcgacacct tccttatatg
     3421 aaatgctgac gttcagtttc agcggcgagt taccgctgga acaggtcagt gagcgggatc
     3481 agcttatcct ttccgtgatg gacaatatgc aacgcattat caactgtcgg gcaggcacac
     3541 tggcgcattt gccggattac ggtctgccgg atctcagcct gatccatcag gggatggctg
     3601 cgggtattca tagcctgatg cgccagattg aagaaacgct gctgcgttat gaaccacgcc
     3661 tgaatcagat acaggtggag ttactgcccc agcccggtcc ggggcatctt aattacctga
     3721 tccacgcgca gcttcccgat accggctgga tacgctttga tggcgtattt tctccggaag
     3781 gacgaattgt tctgcgtcat ctcaaacaac aggagcgggc gtactgatgg caagtaacgc
     3841 gaattttatc agccagttcg tcatgggcgg cgatccctgt acttataagg aatccggtga
     3901 actgcaggct gaaatgagta aactgactca cccggcccga ccggatgtgg actggcgtca
     3961 ggtggaaaaa ctctgcctcg cgctgttccg gcaaaatggc gtggaactac agacgctggt
     4021 ctgttacgtg ctggcaataa ccagacggca ggggctggca gggatggcag acggactcgg
     4081 ttcactggat atactgctcc agcgctgggc tgacttctgg ccggtacagg tacattccag
     4141 aatatcactg ctcagctggg tcacagaaaa aatgcagcag gcactgagaa cgctggatat
     4201 tcagtatcag gatctgccgc agatctaccg ttgtgtacag catctttccg ccatcgaaac
     4261 cacgctgcaa cagtgtgaac tgtggcatat gacgaagctg gacgtattgt ccgggcagtt
     4321 tcgcaatgcg gctctgcgtc tggaacggct ggcgcctcag ggagcggaaa ccattatcac
     4381 tccccctgaa ttaccccgcc gggaaatgaa tcaaccgaaa aagtcagagg aaagtccaca
     4441 gccggttttt gcaaccagat ccgttcagca aaacgataag gatgccagtc cacccgtgcc
     4501 atcccctgaa atctcccggc agcggacatg gccgatattt atggccggaa tggttgtgat
     4561 ggccggtctc ggcggaacag gattatgggg ctggtcgcag cttaatcagc cggacgcgct
     4621 aatccagcga atacaactgt ctgtcatgcc gttgccgcag tcgctggaga gcggcgaact
     4681 ggcaaagctg gatgtaaagg ataaggcgct gctggctcag gacagaacaa ttgcggcaag
     4741 tcagatgcaa ctggagcagt taaacaaatt gcctgcccgc tggccactgg agcagggata
     4801 tcgccagcta cgccagcttg atgccctgtg gccggataat cctcaggtca gggcgctgaa
     4861 cgcgcagtgg cgcaaacagc gggagctgag cgccctgtct gctgaggcac tgaatggcta
     4921 tgctcaggcg cagagccagc tacagcgcct gtcggcgcag ctggatgcac tggatgaacg
     4981 taaggggaga tatctgaccg gttcggaact aaaaacggcg gtgtacggca tccggcagtc
     5041 gttaaaggag ccgccgctgg aagaactgct tcggcaactg gaagagcaaa aacagaccgg
     5101 agaggtttcg ccaacgctgt tgacgcaaat tgatacccgg ttaaatcagt tgttgaatcg
     5161 ctatgtcatt ttactggata cgaaggtgga acaaagtcag taaacatcgg gggatttatg
     5221 taccaataag ggatatgtga ccttgccatt ttttaaaatg gagtctctgt gtacaaaaaa
     5281 agagaggaac aggccattcg atctactcgt tgattcacat cagataatga gtgtatattt
     5341 cggtagatag cttgggttcg ggaacagtga ataccttaag tttcaaaaga ttggattaac
     5401 ctcttgactg tggcccccgc aaccaattta ttagaatgct gacaaaccgc aatgtgttct
     5461 acaaattgcg gtttttttgt atttaaaatc tttgttcgct gaactcacaa cacgtttttt
     5521 atctcccttc ctgcgaagta atcaattatc gactgggcaa cgtgtatccc ggtttgctgg
     5581 tatgactcaa cggtggcggc cgcgatatgc ggcgttgtaa tgatattttt ggcatgtagc
     5641 agtgggctaa attgcgccaa cggttcagag ctaaacacat ccagcgccgc aaacacattt
     5701 cgttcttcaa tcacctgaag taaagcgccc tcatccagca actttcccct tgcggcattg
     5761 atgatcagag caccattgcg catcatcgac aaatatttat gactaatgaa gttctccagc
     5821 tcagaagaaa aaggcaaaag aacaatgatg acatgcgact gcataaagat atcttcaatt
     5881 gatacaaact gaactccagc tttgtcagca acctgatagt cgggttgttt gtcaaaagct
     5941 aaaagctcaa catcaaaacc agataacctt ttcgccagtg ttttgccaat attgccgtag
     6001 ccaagcaggc caatacgttt accttgtaac tcgcagccat gcgactcacc ccaataacca
     6061 ttttgcattg catgatagct accggggata tttctcatgc tcgcaaaaat aagaccgatg
     6121 ataaattccg cgactgcatt tgaattaatc ccaacagcat tattgaccac aatacctgat
     6181 tgtcgagccg tatgaagatc aatattatca atccctgtcc cgaatctcga tattattttc
     6241 actcctggta gtcgcgataa cgtatccgct tgataacaat cttttccggc aatgactgcg
     6301 caaatagatt ctcgtaatac tgtggattct gatttaacca gatgctccac attattcttg
     6361 ataataaggt taaaatgatt ttctcttagc agcttgctgg cagaaacaca acgcgctgaa
     6421 aagctgggaa cggtgaccag aacgtttttc atagctgaca ctcacatcaa tgaacgccac
     6481 cccaaaagat ggcgtgaccg gttactgttt tatattctaa aattatgagt ttgctttaat
     6541 accgagcgca cctcctggat gatataagcc gaaatcagca cgagtaaact ttttcaattc
     6601 ggagagcgca agcgccaccg catcaccaac aaccagaacg actgttgatg agcatgacgg
     6661 cgctaaacct aaattatccg cctcactttt tacgggaatt tcaacggata aatcacaact
     6721 tatcgcaagc gatgagcgat gactgcgggt aaaggagatc aaataattac ccattttttt
     6781 cagactaggt agtgttgcca ggatttctgt cgtctcacca ctgtttgaaa taagaataac
     6841 aacatcatct ttcgccacca tcccaagatc gccgtgtacg gcttcagtac cgtgaacaaa
     6901 aaacgaggga gttccagtgc tggcaaatgt tgcggcgagt tttcgggcaa taataccgga
     6961 cttaccgaca ccaataaaaa caactttccc ctgacaagac tgtaatactt taattaaatt
     7021 tgcgacctcc gaaagggggg aatcaataac ttcttcaact gctttatgtt cgttacgcat
     7081 aaaatgttta atgagatgga taagatccgt actgttcatt tttattttcc ttgattaata
     7141 tttttaatta cactcgcaag acgcgtaagc cccatatata atttttctcg accgcaggcc
     7201 aggttcagac gaaggtagcc ctccccctcc gttccataga cattccccgc cataatgccc
     7261 actttgcctt catgaattaa tcgactgtag aattcatcaa aggaataacc aatagcagag
     7321 caatctatcc acgaaaaata gcaaccatca gctggcctta cgatcaattc tggagtattt
     7381 tcgttcagat actgctgggt gaattgaatg ttagaggcga tatgtgcctt taatgacttt
     7441 aaccatccct cacattcatt ataggcaaca atggttgcaa ttatattcag tactgatggg
     7501 gagtttacga aatcgcgata cctcgatatc gttctgaact tatgattata atcctcgtca
     7561 gttgaaatca gatacgatcc tgttagcgca ggaacattaa atgttttcga tatcgagtta
     7621 aataccacta ctttttcttt ttttatttta agcgcggatg taaagctacg attaaaaaca
     7681 aaatcactat gaatttcatc tgaacataac cagacattat gccttgtaca gcttgtagcg
     7741 attttaagca gttcttcttc cgaccagact gttcctgtcg gattatgagg attacataat
     7801 aacaccatct ttgcgctttt aatcttaaga tcaaaatctt gccagttgat agcgtagccc
     7861 ccctcatttt ttataatcaa agaggctgtc agaatatttc gctcgctatg tttaattaca
     7921 tcaaaaaatg cattatagac aggtgtaaaa acgagaatat tatcaccatg cgatgtcaat
     7981 aactctattg cctttgcaat agagtacatc acactggggc tataggttat ccattcaggt
     8041 ttaatgtctg tttgatattg ttgatgatac cagtttacta tcgcattcag atattcacca
     8101 tgttgccaac gactgtaccc taaaatcggg tgttccaggc gtttttttaa tgctctggtg
     8161 atagcgtctg gtatcggaag atccatatct gaaatagaga agggtaatac atcttttaca
     8221 ccaaatctgt cctcgcaaaa gtcccattgg gtgcaatatg ttcctttacg ggaaatagga
     8281 gtattaaaaa tatccatcag cgcatcccca tgtagttatc catatcccta cgcagggcga
     8341 tgactttagt gccgataatg acttgtaggg tattttgatc aagtttaaca accgcaatcc
     8401 cgccagctct ttttattgca acttcatcaa tcaatcccat atctgcgatt gttaaacgta
     8461 aacgagttac gcaattatcc agtgacttaa tattctcttt accaccaagt gccttaacca
     8521 tctgctccgc tgcttgcaac tctgatttat caagagttgg ggtatccgct atagccgtat
     8581 cttcgtgagc gttcagcgtg acaggactgg actcttcacg tcctacggtc atcaggttaa
     8641 atttgagaat ggtccaacgg aacacaaagt aataaatgaa gaaataggcc acttcaacac
     8701 acaccgcgat cggccaacct gttcttgtgc cacgcaatac gccaaagcta ataaattgaa
     8761 tcagatcgcc agtaaatccc atcttcacgc ccatatatgg caagaccatg ttggcgaatc
     8821 cattcataaa ggcatggaag acaaacagca gcggactgac aaataagaac agaaattcaa
     8881 tcggttcgct gacgccacca ataataaccg taagcacacc ggaaataagc aatgctttga
     8941 tagcctttct gttttgagca tgagcagtgc gatacatggc cagtgcagca ccagccagac
     9001 cgtactgaat catcagtttg ccctgctcca ttttccccgc caaatccagc ggaatgaccg
     9061 cattctcttt gacgtacgcc ataaacatgt tcagggtgcc gtaatactct tcgccgccaa
     9121 tcacagccga accgccgatt ggcgtaaagc ggaacactga cgtcaccaga tggtttaagc
     9181 caaaaggaat cgtcacacgt tcggcaactg catagaagaa ataaccaaca ggaccggaag
     9241 ttgatatcca gtggccaatg agcatgaaga gattgaaaaa tggcggccag ataaaaggaa
     9301 tgatcagacc aaatccactc atcacaatca gtgtaatgat aggcaccaga cgtgggccgc
     9361 tataaaaacc taatgattct ggaatgcgta aattaacgat ctttttatac atgctggcga
     9421 ctaataaccc agcaacaatt ccccctaaca cgctggtatt gtaggactgg atccccagaa
     9481 tgatgttttg cccatgtgtc gacatttggt cagcaacgac caataagtcg tgctgtttaa
     9541 gataaaagtt cgttcccaga tgcatcgcca taaaaccaat taagccagaa aaagcaccat
     9601 aggctttatc ctctttatct tttaataatc ctaagggaat cgctatcgca aacaatacag
     9661 gtaaattaac aaaggcaaac aaaccaagac taacaatgaa atcaagtatg gttttaatta
     9721 ttggaatagc cagaaatgga attaactttg ccatatcatc actggctaaa ccacttccca
     9781 gccctagcat catgccacat acacttagca gagcaatggg atacataaat gccttcccca
     9841 ggctctgaaa aaaactccag gctttctttt gtttcatgtg ggttatctca tataaatgtt
     9901 atatataatt agtccattaa tactttggta cgaatagaga gatatagttt ttcttctaaa
     9961 attaattcat atttaaaagt ggcatacaga taccgttcaa tttcatgaat tgcgcgctgt
    10021 aacaggatgt ccttttcaag aaactgttca tagatatcac caatatcttt ctgttttatt
    10081 ttttcattgc ataacacgcg acttgaaaaa tatttaaggt gcatcataaa tctggaatag
    10141 ttaaatgaac ttctgtccac ctttctgtta taaacaactt caataagctc cgtgattatt
    10201 tgcgacaact caataatccg tactgattca taatcaactg aattttcata attatttaaa
    10261 atatgcaatg ctataaaacc aatctcatca tcaggaagtt gaacgctgaa ttgctcatta
    10321 atgatagtta atgcatcgcg acttatcgca tactctgctt tgtacaacgc tttgatttca
    10381 tcaaggaaaa cattattaat ttgtatacct gaaactactc gggatattgc attcgaaatg
    10441 tgatccgcaa gaatcaatag tagcgatgga ttgagatttt tttcgagatt tttctccgca
    10501 tattgcacta ttttttcggc aacaaaaata tactcaacat ccacatgttc aatcaatttg
    10561 tagagtttat ttttttgttc gtttctgaca taaaaaatcc ggttagccgg atgttcaggg
    10621 accgacattc catatttttt gttgtaaccg acgccaggtc cagaaatgat aacctcctgt
    10681 ccattcatcg atgcctgtac acaattattg ttcatgactt tttcgatgat cattccatga
    10741 ctccaaaaaa aaaggcaagg cacgtcagaa gtaataatta cttcaaacat tgccttgcct
    10801 gatttaacag ttacaagatg attaactaat gcgtatacat tacacggaat tgtgcttaat
    10861 taaagagatg gtcggcttat ttttcaagcc gacattcaaa tattcgacat cagtcacaaa
    10921 accagcatct aatattcatg aaaatgttat ctattcaacg aaatataaat tcattgaata
    10981 aataaaaaca tcatcccctt ctcgccagcg tcgccccatc ggcaaaatac gctttaatcc
    11041 ccgcaagaat agactccgca acttcctgct ggaaagtcgc cgttttcagt ttacgttctt
    11101 cctcaacgtt actgataaac gccgtttcga ccagaatgga gggaatatct ggtgccttta
    11161 gtacggcaaa cccggcctgt tcaacttgat ttttatgcag cttgttgatt ttaccgagct
    11221 tattcagcac cgctttacca aacttcaggc tgtcggcaat ggtcagcgac tgcaccatat
    11281 cgaacatggt gtggtcgaca tagcggtcac cgcttttgct cacgccacca atcaagtccg
    11341 aggcgttctg ggtttgtgcc agatattttg ccgcagtact ggttgcacct ttggttgaga
    11401 gcgcaaacac agaggaaccg ctcggctgac gactggtaaa ggcgtcggca tggatagaga
    11461 caaacaggtc agcacgctgt ttctgggctt ttgctacgcg cacttgcaac ggaatgaaga
    11521 tgtcttcatt gcgcgtcatg tacaccttca tattgccctc tttctcgatc agagagcgca
    11581 gacggcgagc tatttgcaat accacgtctt tttcgcgcgt tttgtatttc cccaccgcac
    11641 cggagtcttc gccaccgtgg ccagggtcaa gcataatgac aataggacga tcgcgccctg
    11701 ctttacccgg ttgtggacca ctttgtgctg gcggcacctg cttttcgagg tcgcctttgt
    11761 tgtaatcctc cagcagcgcc agcagcgggt cctgcatatc ctgtgcattg gccggataga
    11821 ggtccatcac cagacgctct ttaaacccgg cgaccggcgc aagggcaaac agctgcggtt
    11881 ttacgttttg ctttaattca aaaaccatac gtacggtttg cgggtcaaat tgcccgacgc
    11941 gcgccgactt gatgaacggg tcgtcagcgc ggatttgcgc agccatcccc ttgagtaccg
    12001 agttcaggtt tacatcttcg atatccacca ccacgcgttc aggattactc aacgcgaact
    12061 gcttatattt cagctgacga tttgattcta ccgtcacgcg ggtgtagctg gacgcaggcc
    12121 agacgcgcac cgccacgacc tggctgaccg cagccaggct gacctgactt acgctcaata
    12181 gccacatggc acccgcgcct tgcagtaaac gacgacggct gattgcagtg ttggatcctg
    12241 acatgcctct cccgagcaaa agaaatctaa gctgtgtaac aagtaaacga ccaatttgac
    12301 cggaaacttt agcgaaagac gcataatctg tcatctataa aacggtaaac attcttttta
    12361 tattcacggt attactgata aaaaactcgc tcccgcataa aatttacact tgcaccctgc
    12421 gaaaaaacag aataaaaata caataatttc gaataatcat gcaaagaggt gtaccgtggt
    12481 aaaggaacgt aaaaccgagc tggtcgaggg attccgccat tcggttccct atatcaatac
    12541 ccaccgggga aaaacatttg tcatcatgct cggcggtgaa gccattgagc atgagaattt
    12601 ctccagtatc gttaatgata tcgggttgtt gcacagcctc ggcatccgtc tggtggtggt
    12661 ctatggcgca cgtccgcaga tcgacgcaaa tctggctgcg catcaccacg aaccgctgta
    12721 tcacaagaat atacgtgtga ccgacgccaa aacactggaa ctggtgaagc aggctgcggg
    12781 aacattgcaa ctggatatta ctgctcgcct gtcgatgagt ctcaataaca cgccgctaca
    12841 gggcgcgcat atcaacgtcg tcagtggcaa ttttattatc gcccagccgc tgggcgtcga
    12901 tgacggcgtg gattactgcc atagcgggcg tatccggcgg attgatgaag acgcgatcca
    12961 tcgtcaactg gacagcggtg caatagtgct aatggggccg gtcgctgttt cagtcactgg
    13021 cgagagcttt aatctgacct cggaagagat tgccactcaa ctggccatca aactgaaagc
    13081 tgaaaagatg attggttttt gctcttccca gggcgtcact aatgacgacg gtgatattgt
    13141 ctccgaactt ttccctaacg aagcgcaagc acgggtagaa gcccaggaag agaaaggtga
    13201 ttacaactcc ggtacggtgc gctttttgcg tggcgcagtg aaagcctgcc gcagcggcgt
    13261 gcgtcgctgt catttaatca gttatcagga agatggcgcg ctattgcaag agttgttctc
    13321 acgcgacggt atcggtacgc agattgtgat ggaaagcgcc gagcagattc gtcgcgcaac
    13381 aatcaacgat attggcggta ttctggagtt gattcgcccg ctggagcagc aaggtattct
    13441 ggtacgccgt tctcgcgagc agctagagat ggaaatcgac aaattcacca ttattcagcg
    13501 cgataacacg actattgcct gcgccgcgct ctatccgttc ccggaagaga agattgggga
    13561 aatggcctgt gtggcagttc acccggatta ccgcagctca tcaaggggcg aggttctgct
    13621 ggaacgcatt gccgctcagg cgaagcagag cggcttaagc aaattgtttg tgctgaccac
    13681 gcgcagtatt cactggttcc aggaacgtgg atttaccccg gtggatattg atttactccc
    13741 cgagagcaaa aagcagttgt acaactacca gcgtaaatcc aaagtgttga tggcggattt
    13801 ggggtaagtg gtggaaatcg ccagatgcga catgcgcaat gcctggaggc atgataaaca
    13861 cgctagcatc gcatcaggta ttgacgctca tcgtcggatg cggcgtgaac gccttatccg
    13921 acctacatca aatcaaaggt gttgatggcg gatttagggt aagcgacgga aatcgccaga
    13981 tgcgcatatg cgcaatgccc ggaggcatga caggacgcgc atgcgtcgca tcaggcaatc
    14041 acgtttattc ccgcgaacta aacaatgccg ccagaccact gcgccgctca gtgcgcgtgg
    14101 caattgccgc acttaaaatg cgctcatcgg catacagcga cagacggcga cgcgcgcggg
    14161 tcactgcggt gtaaaccagc tctcgcgtta ctaccggcgt gcgttgactc ggtaaaatca
    14221 acgccgcatg gtcgaactcc gatccctgcg atttatgtac cgtcatcgcc cacgtcgttt
    14281 cgtgctctgg caggcgactc ggttgcacag acttaatatt gccgtccggc atcgcaaacc
    14341 aaacgcgcgt cccctgcccg cgatccagcg caataccgat atcgccatta aacaacccaa
    14401 gcgcgctgtc attacgggca atcatcaccg gtcggccttc gtaccaacga gagtgcggat
    14461 tacgatgaat tttgcgcttc tgttgcataa actgctcaat tcgctcattc agtccagcca
    14521 cgccaaacgg cccttcccgc agggcgcaca aaagctggta ctcattgaac gcctgaatga
    14581 ttaaatccgg ctcggcacgc gcttgcagca gatccagata acgcccgtaa cccgcaagag
    14641 cctcctcaag catcgcaata tagtcttcgc cgctctgtaa gagccgtttt tcgatatcag
    14701 taaaatcctg ctgaaaaacg gttttcactg ccgttttatc accacggttg atcgccgcag
    14761 ctaactgacc aatgccagaa tcgctgccga aacgatagct tttttgcagc aggcagagac
    14821 tgtcgcgcaa agatgccgct tctgtgccag ttcctgacgg aacgtgagtt cccgtcaggc
    14881 ggctcaactg cccggcacgc tcggcggtaa agcccgcgtt ggcataagcg cagatatcgc
    14941 ccagcacagc tccagcctca actgaggcca gttgatcgcg atcaccgagg aagatcactc
    15001 gcgcatgatc gggcaacgca tcgatcaggc gcgacatcat ggggagatcg atcattgacg
    15061 cttcatccac caccagcaca tcaagatgca gcgggttacc ggcatgatga cgtaaacgct
    15121 ggctacccgg ctgcgcaccc agcaatcggt gcaaagtgct ggcatcttcc ggaatgcgtt
    15181 tcttttgttc atcggtcagc ggtaactggc gcaaagcctt gccgagagat tcggttaagc
    15241 gcgcggcagc tttacccgtt ggtgcagcca gacggatacg gcagcgttcg ccgtctgcca
    15301 tttgaattaa cgctgccagc aacttcgcta cggtggtcgt tttaccggtg ccagggccgc
    15361 cggaaatcac cgagatccgc cgcgtcagcg ccactgccgc cgccactttt tgccagttaa
    15421 tttcatcgct tactggaaaa agtttgtcca gggtttgcgc cagtagagct tcatcaacct
    15481 caatggcatg attcacttcg ttgaaaaagc gtgccactgt gcgctcgtta caccacatgc
    15541 gattcaaata aagacgatca ccacagagga tcatcggcgt tggttcatct ccccggctga
    15601 ccgcttgtga agccagcaag cattcttccc aattttgtaa ctcaccgatt tcgctgacgc
    15661 aggttgctaa cagcggatgt gacgcctcgt tattttccag tcgtgagagc ggtaaacaga
    15721 catgcccttc cccggcgtcg tggcttaaca gggctgccgc gagggtgacg gcaggatgtt
    15781 catctcccgc cacggtcagg gcaaattgca catccagcgg gcgtaactgt ttgtgctcca
    15841 cagcttccag taattgcttt tgcaatttca ttacgcctcc tccagggtga taccggcaaa
    15901 catctcatcc atcaggtcaa tcaacccggc gttgggtcgg gtcgcgtaga tcccttgttg
    15961 cggatgttct ttatcaacac cacgcaggaa cagataaata acgccgccaa agtggcgctc
    16021 atagtcgtaa tcagcaatgc gatggcgcag ataacggtgc agcgccaggg tataaagctg
    16081 atattgcaga tcatagcggt gtgcctgcat tgccgctgcc atagcctgtt gggtgtaagc
    16141 agaactgtct tcacccaacc agttggattt atagtcgagc aggtaataac gcccttcgtg
    16201 gcggaacacc aggtcaataa agccttttaa catgccgcgt acctgcatga actccagcgg
    16261 cgggcagcca gcggatagcg agtcaaactg gcgaattagt gcatcaagct ggctggcgat
    16321 gagcggttcg ctaatcggca ggtaaaactc catctccacc tgtttatcgc ggtcggaaag
    16381 ctgactcagg ctaacacccg tttcattgag aggtgcctgg aggacagccg tgatccactc
    16441 ggtcaacacc ggttcccact gcgattcaaa gccgccgagt tccagttttt cctgtaccca
    16501 gttcgggtca accggctggg taaaatcgag gtcttcaaac aaactgtgca agaatgtccc
    16561 cggtgacgca ccgcgcggga actgatgcgg tgttaacgtc ggttcgtcaa cgacgctgac
    16621 cacgcctgcg gcatcaacat ccagccgtgg catcagatcc tgagcgatac cgtgaccacg
    16681 ctgctgcaaa ccggagtagc tggtgacacg ccagttatcg ccgggcaatc gttgtagcgt
    16741 cctcgcattc agttctgcag ttatagcatc attaacctgc cagggctggt tatcaccagt
    16801 ttgtgccgtt tgccaggcaa tatcatcatc gcataacgct tcaatacagg tgcgaagccc
    16861 tgccgcatct tgcggttccc ctttttgcag caaacgcccg agcgcacttt ggtggacgtc
    16921 ggtgtcacct tttttatcgc cacgacggcg caccagcggt gcaacgccga gactgcaatg
    16981 ccaaaccgaa cgcgtcagcg ccacgtaaag caaacgcaga tcttccgcca gacgttcggc
    17041 ctccgcgaga tcgacgcttt ctggcgcagc attaagatcc agaacagcct caaacgagtg
    17101 gcgatcgtga tagaacgcct gatcctggac gcggaaattg gtaataaatg gcaaccagac
    17161 cagcggatat tccagccctt ttgatttatg gatcgtgaca atctgcacca gatgtttatc
    17221 actttcgagg cgcatttgtt ggctggaggc attactgtct ggctcgagga tatgttgcga
    17281 taaccagcgt accagcgcat gttcactttc cagctgcgtt ccggcttctt gtagcaattc
    17341 gctgatatgc aagatatcgg taagacgccg ctcaccgcct gctgttgcca gcaagttttc
    17401 tgcaatgtta cgcgccgaca tcagcgcccg cagcatcggc ataacgccac gtttgcgcca
    17461 gatttgccga taaccatcga actcttcgac gaccgcatcc cacgcatgtt cgtcattatt
    17521 cagcgtttca atatccagcg cattcagccc catcattgac gttgccagcg cactgcgcag
    17581 ggtgttctca cgttcgggag tcatcaccgc ctgcaacagc caaagcatct cctgcgcttc
    17641 cagagtttca aaaacactgt cgcggttcga aaggtaaacg gaagggattt ccagcagcat
    17701 taaggcatcg cgcacctggg cggcctcctg gcggctgcgc accagcacac tgatgtccga
    17761 agcacgcacc ggacgcgcgt cgtcgccgtt catcagcaac gcttcgcccc gctgtccggc
    17821 ttgtagccag tcgcggattt gcgcagcaca tacctgcgcc atggtacttt gatagtcgcc
    17881 aacgccgcag ctttcgcctt ccatcagcca cattttcatc gcaggctgcg tttcaccttt
    17941 aaacacaaaa cgtaacgcct gatttttgcc ggcagatttc acaggaataa acggtatttc
    18001 acgaaacatg aacgcgtcat cagtctggct gaaaagtttg ttaacgctgt tcaccattcc
    18061 cggtgcggaa cgccagttgg tatctaaggt gtagtgagca tgaacttcgc tacgcgcctt
    18121 catataagtg aagatatccg caccccggaa tgcataaata gcctgtttcg ggtcgccaat
    18181 cagcaacaat gcggtttccg gctgatggtg ccagatacgg cgaaaaattc gatactgctg
    18241 ggggtcggta tcctgaaatt catcgatcat tgccaccggg aatcgcgtac ggatcgccgc
    18301 tgccaatacc tcgccgcttt cactacgcag cgcggaatcg agccgactta acatgtcatc
    18361 aaaacccaat tcgccacggc ggcgtttttc acgcgctact gtttcgcgga tctcagccaa
    18421 tgcgcgggtg atcaccagat cgcggatcga caatggttct gcaagcagtt gatcgatcgc
    18481 ctcaaacagt ggatgtcgcg gggtttcccc cccggctttc gtgcgatctt ctaagaaacg
    18541 ctgagagaat ttttccagcg actccggcaa ctgatagctg ttggtctctt cttctgccca
    18601 ggcgctgatc ttgtcgatcc atttagcctg attgctacgg ttaaacttgc gtcgatcaat
    18661 accagaagat tcgatcagcg catccagttc acccactgcg tcgcgccact gctgttttac
    18721 ggcatcaata cgcgccacaa tttgcgcgtg gcgggaagca agcgtttcat catcgggcgg
    18781 cggtgctttg ataaccggtg cttcgccttg cagataacga ttaatatcgc gcagcaacgc
    18841 ctgcggccct ttccaggttt caaagaccac ctgggcaatt tcacgcggca gcgggtagca
    18901 gtggcgacgc cagaaatcgg cgcaggcctg gtagcgtagc agagactcat cttcaatcag
    18961 ctgctgctca aacagcatgc cggattcaaa ggcattcagg ttgagcatgc gctggcaaaa
    19021 accgtgaata gtaaagactg ccgcttcatc catctgccgt tcggctaaca acaaccactg
    19081 cgcggcttgc gctttatcgt cgatctcctc cagcaggagt ttgtacagtg gattgtcggt
    19141 ggtttcacgc agacaggcga tgcgcaactc gtggatattg ctacggatac gaccgcgcaa
    19201 ttctgccgtg gcagcctcgg taaaggtgac caccagcagt tcttcaacgg tcagcgggcg
    19261 gggaaaggcg gcagaaccgc ctagtccaag taacaggcgc aaatagagcg ccgcaatcgt
    19321 aaaggttttg cctgtgccgg cagaggcttc aatcaggcgc tcgccctgta agggcaagcg
    19381 caaaggatct agtgtctcgg cgacatcact cattcttttc actcatcagg ggcattgttt
    19441 gctgcaacgc gctgacgttc tcccacactt tccagccttc aggatgcaca tattcggctt
    19501 tcccgttctg gctgccggaa atctgcgaca gaatagccat gccttgcggc tcgaccaccg
    19561 cctgatggaa gaaatcagca agtttttgcg gcgtcagcag ttttatctgg gccacgattt
    19621 tatcacgcga atcgaagcgc atattgccgc gatcgaaatc tttacttaac ttcgatgctt
    19681 cttcgccgag cgtttgcggt gcctgcagca tctgggttat taccgcctgc tggatttgcg
    19741 caaactcctc tggcttcatc gcccgcaatt ttgcctctgc ggttgggaaa aacgccttgt
    19801 aacgctccca caagaatgaa ggctgtttat cattgctttg caaaaggaag cccatgcccc
    19861 actgacgccc cacgctcatt ggaaacgcaa acacggcata gcccaactgt tcttcggtac
    19921 gcaactgatt gtagaaccac ggctgtacga tctgccccaa cagagagcta taggctgagc
    19981 tggtgtattc atcgtagcca gtcggtacaa ataccgctgc cagtgcggag tcggtgctgt
    20041 taccggcttt ttcaaagatg acagattgtt ttttatcgac cacgacatct ttgttacggc
    20101 accactccga accatcagcg cccaactgtt tttgcacatc gcgtgccagc gttgttgcct
    20161 gggcttcggt catgttgcca ataaccataa actctggtcg agcccctgat tttaaggcgt
    20221 cgcgataggc cagcacctct ttcaaagtga ttgaaggcaa aattttgcgc cgttcatctc
    20281 gcgagaagta cggcacttgc gagagcatct gcgcgggcat aatcgcctgc tcgaacgctt
    20341 tgcccttttc tgcggaatcc atcatctggt tataccagga cttcgcctgc tcaagctgat
    20401 cttccgtagc ggtatagcta aagtacccct cgagcaatgc ctggaacagc tgcggcagac
    20461 gctgggtgta accattagca ttaaccataa ggccgttgtt agcgttggtg gaaaaactaa
    20521 tgccaccaac cgacgcctgg ttgcttaact gatcaagcgc tagcccggca agataatcat
    20581 tgagcgcaaa catcacctga ttgcgggcgc tgtccatggc tttcggatta cgcaaaatca
    20641 ggctgacatc agctttgggt tcgctggaaa aataacggct tggcgcatag accacacgca
    20701 gattcgactc atcaacaata agctctggat ggtcatattt cttctctgac ttaatcagcg
    20761 agaaatcgtc aggaatatag gggttaagct ctggcaaaga gagcgcaatg tcggcggctt
    20821 ttttctgcca gtcagcgaaa gtttgtgcgc taattttatc gacctgatac ggcgcatcga
    20881 caaagtaagc cgttttgttg tgcggctctt tcgggctgat ataccagata cgcgcattct
    20941 gcggcgtcat catcgccagg cgttctttta ctgctttagc gtcgtaccga tcggcaatat
    21001 tgactgcatc cagcgtatgc tcaacaggaa cgcgaatcat ggtatctgcc agccattcga
    21061 cgtaatccat atcacgggtg attgacggat aacgaaagtc gatatccagc acattcgcca
    21121 gttcatcgaa gtattgttta tcaatccctt tttcgcgtaa cagattgagg tagctaaaaa
    21181 tggccgccac aacctgatcg cgattcgcca gacctttatc ggttaaagac gcagagatcg
    21241 ctaatacgcc gctgttgccg ttgacgatag gatcggaatt ggcgctaatg ccctcaacta
    21301 atccctgctt ttgcagccag tcagaaagtg tacctgggct gcgattaccg atcagatagg
    21361 taatcaattc atccgtttta ctacggaact tcgctgaatt gttatcgatg cgaaactcaa
    21421 cgcgcagaac tttacgcggc agcgccggga cgtaatgaat gataatacct ttttgcgcgt
    21481 cggtgactac cggcacggtg atttccggtt ttttgctctc tttgttcggc acgcgaccaa
    21541 aggtgtccgc cgccattttt gccaactccg gcagcggttt attgctgtaa ataaccgcct
    21601 tcatcagatt ggcagagtaa tatttctcgt gaaaatcttt cagcgcctgc tgcaccggat
    21661 ttccgggttt gtcgcttaaa gtttcgaggt taccaccaga aaactttgaa ccggggtgtg
    21721 ccgggttaat ggtttctgcg ctgacctgtg ccatgcgcat cccgtcacgc gtacgcgcca
    21781 tggttaattc ggcattcact gcattacgtt cacgttcggc gtatttcttg tcgagcaagg
    21841 gttctgcaat agcgtccgcc aggcggtcta ccgcaccggg caaggcgtcg ttctcaactt
    21901 ccagatagaa agccgtgcga tacggtgccg tgctggcatt gtgactgccg ccgtgcattt
    21961 tgagatattc ggccagactg tcagcctgcg ggtacttttt cgaccccatc agactcatat
    22021 gttcaaggta atgtgccagc ccctggtacg cctcgggatc ttccagcgac ccaacgggca
    22081 ctaccagcgc cgagagcgat ttaaccgcct gcggatcaga aaccagcaag accaccatac
    22141 cgttatccag acgtatagcc tgatactggc ggttatcttt atcactttta cggatggttt
    22201 cctgaatcgg ctgccatccc gtttctgcct gacttaaggg tgcccaaagg gcaacaaaca
    22261 acaataatgc tttgaaccag gtgctgcggg gcattcacgg acctcataat caactttatt
    22321 ttctggccag attcaacagg ttaacgcaaa tcatctgcca gaatttaatc ttgtgctgaa
    22381 cgagtcagcc tatgtctata tatccatcag tccgtgactg gtgcgcatca taaagcaagc
    22441 agatatattg cgcaattttt atacagcact catgactgat taaagcgaaa cagcggtaac
    22501 aggaaacgtt gcgactgttc aacgatagcc tccattgtct ctggtgttaa ttgccgccag
    22561 agccgttgat accagatatc atcaccttcg ccacgcacca tcatgttgcc ttcgtaagcc
    22621 tgaaggaatt tcgtacgggc tttttgtaac gtggaatcgt catccagcat ggcatcgttt
    22681 tgcgcgtcat aacaggtttt tagccacgcg ccgccacttt caggtaacac cagcaatggc
    22741 gcggacattc cttcacgata cccctcaatc agttgtgaga ggtaatgcaa agcctgttcg
    22801 gctgcaagcg gcggaaaacg ccactcgccg tctttgcgta gaaaaaggcg actttcacca
    22861 ttaccaccgc tggcacagta gacaaggtgt tccagccaaa gttgcattcc ctgcgccaca
    22921 cttaataaag agggacgcca gcgcaacagg ccatccggct gcacctgcgg caaccagcca
    22981 gttatctgca caccgttgca ggcgagatca atttccatac tctgccccgg ctggcgacgt
    23041 gcaatgactc tgtcggcaag ctgctgcatc tcctggcact gtgtttccca gaaaatttca
    23101 ccaaaagcgc catacggtaa atcccctgcc gcccggaagc ggcggaataa gcgatcggca
    23161 tcatcctgct caaccagtgc attcaataac tgctgattga tttgataacg gctaagccct
    23221 tccagaataa atggctcggt gtcggggatt tcgctgtctt cagtacggaa gttcacctgc
    23281 aaacgcatct ggaagaacgc ccgtaccgga tgtgcccaga atcgttgtag cgtttccagc
    23341 ggcacggttt ccggtaaggt aaacggcagc ggctgaacaa attcagaatg tgctttacca
    23401 gcctggctgg ccgcaggtag ccattcacga gcatagcttt gtcgttcgcc tggctggtag
    23461 ttttgcggat caaacggcat ccgggtatgg aggcaagtaa gatgcgcttt tacccttgcc
    23521 tcgctttcat cacagttgag cgcttcatcg cccggtagat aatggctttg cccgatgtag
    23581 tcgatcagtt cctgcaccag taccgacggg aaacgttcac tgttatcctg aatagaacgc
    23641 ccgatatagc tgatatagag tttttgctgc gcggaaatta acgcttccag gaacagatag
    23701 cggtcgtcat cgcgacggct acggtcgcca cgcttcggtt tctggctcat caggtcaaag
    23761 cccaatggcg caagctggcg tggataaacg ccgtcgttca ttcccagcag gcaaaccact
    23821 ttgaatggga tggaacgcat tggcatcaga gtacaaatgt taaccggtcc ggcgagaaaa
    23881 cgctggctga tacgttcctg atccaggcgc tgtgccagtt catcacgcaa tagtgacagc
    23941 ggcaccgcgt cgccatactg cgcacctaaa ccttcggcga taatcgcctg ccattgttgt
    24001 tcgatcagcg tcatagccgc ttcggtttcc gcatccggca ggaagaaggc gttgagcata
    24061 tcgcgacaaa ccggcaacca ctcttccagc ggacgctcct gcgccagccc gcgacgccag
    24121 atgtttagct gcattagcag tgaagccaga tgccccacca gttctgcaat taagccgctc
    24181 gattcatcat aaggtagaac cgattgccac tcgccctgcg cgctctccat cgcgtagccc
    24241 agcaacatac gcgtcaggcc aaatcgccag gtgtgttgtc cggtggcggg gagttccagc
    24301 tcgcgaacgt tgtcgtcatc tatcccccaa cgaatgccgg attcgttgac ccactggcgt
    24361 aaataacgca gcccttcttc ggtgatgtca aaccgcgccg ccagcaccgg cacatccagc
    24421 agcgccagca catcctctga cacaaagcga ctgtcaggca gtgataacag gctgataaac
    24481 gcttccaata ccggatgtga ctgccgcgcc cgacggtcgg aaatggcgta aggcaggtaa
    24541 cgatccgcag gtgcgctacc aaacacagcc tgaataaacg gactgtagct gtcgatgtcg
    24601 gccaccatca cgatgatgtc gcgcggagtc agtgtcgggt cttcctccag catcgccagc
    24661 aggcgatcgt gtaaaacttc aacttcacgc tgcgggctat ggcaaacgtg aaaggtgata
    24721 ctgctatcca gtggatcaag cgggcgtttg ttatcgctac gggaaaactc ttcgatgttt
    24781 acaccagcaa cggcgcggtt ttccagttcc agaatgtcag actgaatgtt atgcagcagg
    24841 ttatctggcg taacatcgac aaaagcgtcc agctcctggc tgctctccag atcagaaagg
    24901 agataaatgt agtcgcgccc aagcttgccc catgaggcca gcagcgggtt gccgacatcc
    24961 tgttcaccat cgctgttaaa gagctgcccg gcattttcgc tgtcgcgaaa tagcggtaat
    25021 tctcgatctt caaaactgtg tcggcgctgg cgagtcagta gtttcgccag ataagcagga
    25081 tctttaatgt cgccccagta ataacggcag gggttggtaa acaggagatg gatttcaata
    25141 tgtttaccca gcgcctggag tgcctggaga taaacaggcg gtaacgcgga aataccgcat
    25201 ataaagacgc gcgaaggtaa ccccggcggg caggtcgtcg cggactccag cgtttcgata
    25261 aagcgctgat agagattggc gcggtgccag cgcggttgcc cgagttcatg ggtatattcc
    25321 accaacgcct tccacaacgg cgcttgccag gcctgtgctt ctcccagccc ttcaaccaaa
    25381 tgtcctattt cccactgtgc cagccagtcc ggacgataga ccagatactg gtcaaacagg
    25441 tccgccgctt ttgaagaaag ctggaacagt tttcgcttgt cgctatcgtc agtcagataa
    25501 tgccgcaaca gggtaaagtc ttcgcgctcc aacagttgcg gcagcagagt catcagtttc
    25561 cagctcatgc tctgtttgtt aaaggcgctc tctttgggga tttccggtaa cacccggacg
    25621 aacatatccc agataaagct cgctggcagc ggaaaatcaa tgtttgccgc aataccaaac
    25681 ttttgcgaca gggtcatttg cagccactgc gccataccgg tactttgcac cagaatcatt
    25741 tctggttcga aaggatcgtc cagccgttcg cgttcgacga taaactccat caacgcttcc
    25801 agcacgtcca gacgattgga atggtagacc cttaacatag cggctcctga ctactgacga
    25861 ttcgggcaat gcaggcgggt catctcgcct tctctgcccc cgggtgaaac tagcgtaacg
    25921 ctgatgctga cacatcccgc ctgcgatgtc tgcattcggt tgacctgcca gttagcaggt
    25981 ggcgaaatcg cgcgcagttg cgtttgctgc cagccatgcc gccagagctg ttggtactgg
    26041 tttctgctgg caagactgtt cattaatgtt cgctggaaac ccgataacgc agtgacaatc
    26101 atcaccatca acaccatcgc caacattacc tccggcagac taaagccttg ttgattcctc
    26161 agggaagctg acataacgcc ctctctttca gcggacaaaa atcgctccag ccgcgtggcg
    26221 aaaaaacaat gtttccatcg atgacttcgc ctgttcgcca caacgaaacg ccttcataac
    26281 cagcaatcaa taaggcttca ttatctgcca gtaaacgcag acaaacccgg gcaccggtgg
    26341 cagcatacag taagcactga actgcgggca gcgtctgcca ggagtgcatt tttccccact
    26401 ccagcgccga ctgaacgatg gcctggcggc gcaatgactg actttccatg ctcacgcgag
    26461 aagcaaaact gcgatcctgc tgactcattc cttgcaatag caagctaccc aaaatcagca
    26521 gcatcaggac cagagccagt gacgaaacac ccttttcgcg gttcacaggt tggatcctgt
    26581 cacgctatag ctggcatcca ccacggtttg cggatcagcc ttgctggcag cgtgcatatt
    26641 aaccgtcaac accggcgaga agccgctgac atcctgacgt acgacctgaa aagtgtcgat
    26701 aatgatggca tccggattag tgactttatc ccagccctta ccttcacagg atgtcgcacc
    26761 gcgtagcgtt tccagcacat gctccttcag acgaaatcca atctggtcgg actcttttac
    26821 cggttcgcga tcccagatac cattactgtt cgcatcccac tgcacaatga cacagtcacc
    26881 ctgtccgaca atttccagcc cttcgcccgt acagctgcca tggcaataac ccgccctctg
    26941 gagatgcttc gcgacggtaa atacccgcag ccagatttca tcttccagcg ccagcttacg
    27001 ggtgttcgtt aaactttcac gctgtaacgc aggcagaaag cgtgcagccc ccagcaacaa
    27061 tacgctactg atcgccatag caatcaacac ttccagcaga gaaaaacctt gctcttttac
    27121 aggcatcctt ctgtttctcc ttgctgacaa agccggagtc ttccccacgg cgaaaccact
    27181 agccaccact cgcccgttga gtttttgaag cgaatatgcc cggcccatgc ggtattgcgc
    27241 aggccaaaga aagcaagcga aggtgtcagg tcgctcattt cgacttcggg ccagcgcggc
    27301 acaaagacca atggtgaact gccatgacag gtattcgccc cagcaacgga actcacaagg
    27361 caccataacg tcccctccct gataagactg atactgtggt cgcggttatg ccagttggca
    27421 tcttcacgta aatagagcaa atagtcccgc gcctggctgg cggtttgcca aagccgttgc
    27481 gactgctgcc agtattgcca gccataaagc ccacttgcgc ttagcatgac cagaatcagc
    27541 atcgcgacca gcgtttcaat cagcgtataa ccacgttgtg ttttcatgcc ggcagtatgg
    27601 agcgaggaga aaaaaagacg agggccagtt tctatttctt cggcacatct tccggactat
    27661 ttacgccgtt gcaggacgtt gcaaaatttc gggaaggtgt ctcgaagaat ttaacggagg
    27721 gcaaaaaaaa ccgacgcaca ctggcgtcgg ctctggcagg atgtttcgta attagatagc
    27781 caccggcgct ttaatgcccg gatgtggatc gtatccttca atctcaaagt cttcgaaacg
    27841 gtagtcgaag atggattcgg gtttacgttt gataatcaac ttcggcagcg gacgcggttc
    27901 gcggcttaat tgcagatgag tttgatccat atggttgctg tagagatgcg tgtcgccacc
    27961 ggtccagaca aaatcaccca cttccagatc gcactgctgc gccatcatat gcaccagtaa
    28021 cgcgtagctg gcaatgttga acggcaggcc gaggaagacg tcacaggagc gctgataaag
    28081 ctggcaagag agtttgccgt ctgccacata gaactggaag aatgcatggc acggtgccag
    28141 cgccatttta tccagttcgc ctacgttcca cgctgaaaca ataatgcggc gggaatccgg
    28201 gtcgtttttc agctggttca gtacagtagt gatctggtca atatgacgac catccggcgt
    28261 tggccaggcg cgccactgtt taccatacac tggcccgagg tcgccgtttt catcggccca
    28321 ttcgtcccag atggtgacat tgttttcgtg tagataagca atgttagtgt cgccctgcag
    28381 gaaccacagc agttcgtgga tgatggaacg caggtggcaa cgtttagttg tcaccagcgg
    28441 gaatccatcc tgcaggttaa aacgcatctg atgaccaaaa atggaaagcg ttccggttcc
    28501 ggtacggtcg tttttctgtg tgccttcgtc gagcactttt tgcatcagtt ctaaatactg
    28561 tttcatggtt cctcaggaaa cgtgttgctg tgggctgcga cgatatgccc agaccatcat
    28621 gatcacaccc gcgacaatca tcgggatgga aagaatttgc cccatgctga tgtactgcac
    28681 ccaggcacca gtaaactgcg cgtcgggctg gcggaaaaac tcaacaatga tgcgaaacgc
    28741 gccgtaacca atcaggaaca agcctgagac agctcccatt gggcgcggtt tacgaatata
    28801 caggttgagg ataataaaca gcaccacacc ttccagcagc agctcgtaaa gctgtgatgg
    28861 gtggcgcggc agcacaccgt aagtgtcgaa aatggattgc cactgcggat tggtttgcag
    28921 cagcaaaata tcttctgtac gggagccagg gaacagcatg gcaaacggga agttcgggtc
    28981 aacgcggccc cacaattcac cgttaataaa gttgcccagg cgcccggcac caagaccaaa
    29041 cggaatgagt ggggcaataa aatcagagac ctggaagaag gaacgtttag tacggcgggc
    29101 gaagataatc atcaccacga taacgccaat caggcctccg tggaaggaca tgccgccgtc
    29161 ccatacacgg aaaagataca gcggatcggc cataaactgc gggaaattgt agaacagaac
    29221 ataaccaata cgtccaccga ggaagacgcc gaggaagccc gcatagagta agttttcaac
    29281 ttcatttttg gtccagccac tgcccggacg attcgcccgt cgtgttgcca gccacattgc
    29341 aaaaatgaaa cccaccagat acatcaggcc gtaccagtga agcgccacgg gtcctattga
    29401 gaaaatgacc ggatcaaact ccggaaaatg cagatagcta ctggtcatct gtcaccacaa
    29461 gttcttgtta tttcgctgaa agagaacagc gattgaaatg cacgccgcag gtttcaggcg
    29521 ctccaaaggt gcgaataata gcacaagggg acctggctgg ttgccggata ccgttaaaaa
    29581 atatgtatat gatccgcgct ataacccccc gcgaatcaac ccgcccatgc cgcgacgctc
    29641 cataaaggct gcaacctgat ggcgaacttc ggtcgccagt tgcgcttcca gactacgctg
    29701 cgcaagattt tctgcttcag caaaatcaat gcgccgcagc aggtatttta cgcgcgctac
    29761 agaacgtccg ttcatagaca gatggcgata cccaagccca atgaggattg ccacgcacat
    29821 gggatcgccc gccatttcac cgcacaaacg gagatcgatt ccatgtattt ccgcttcccg
    29881 ggcgatcatc gccagagctc gtaacattgc aggatgaaga ctgtcataaa tgttcgccac
    29941 ccgggtattg ttgcgatcaa cggccaggat gtattgagtc agatcgttgg tgccaacaga
    30001 gatgaaatcg acccgctttg ccagatgcgg cagcataaat accattgacg gcacttccag
    30061 catgatgccg atacgtggtt tgggaatttc gtaaccgatc atctcctcga cttcacgtcc
    30121 ggcacgttca atcaggcggc gtgcttcgtc aacttcatcg aggcttgtga ccatcggcaa
    30181 cagaatattc aggttgcccg tagcggcatt agcacgcagc atcgcccgca cctggatcaa
    30241 gaagatctcc ggctgatcga gcgtaatgcg aatcccacgc caacccaggc atggattctc
    30301 ttcgctaatc ggcatgtaag gcagctgctt atctgctccg acatccagcg tacgcaaggt
    30361 gacgggttta tcattaaaca tttgcagcat cccctgatac tgcgccacct gttcttcttc
    30421 cgacggaaaa ccactttgca gcatgaatgg gatttcagtg cgataaagtc ctatgccatc
    30481 aatacggctg cccagttttt cttcatgttc cgggcttaaa ccggcattga gcatgacttt
    30541 aatgcgctcg ccgcttttta actgggcggg taaattgacg tcatcttccg ccagacggct
    30601 aagctcgatc tcttcactaa ttagccgctg atattcttgc agcagtaccg gttccggatc
    30661 gaccagcaat tcaccgcgat aaccatcgac gatcagcgtc cgacgatgca gtaccgaagg
    30721 ctgaatatcc gcgcccatca cggtaggtat ccccagcgca cgtaccatga tcgcagcatg
    30781 ggagttggca gcgccatcgc gcacgacaac accgactaag cgatcctggg gcagctcagc
    30841 aagcgttgtc gctgacagtt catctgccac cagaatgaaa cgttccggcc aggcgttcgg
    30901 cccttgatta gcgtcatcaa gatgaaacag caatcgctga cccagcgcac gtaaatcgcc
    30961 agcccgctct ttgagatagt tatcgcttag cgcggcaaac tgttcggcaa atttttcaat
    31021 gaccgttttt accgcccact ctgccaccga gcctttatca acctcggcaa acaattcgcg
    31081 acgcagccgg gtatccgaaa gcaggtgtga gtaaagatcg aaaatagccg ccgtttcttt
    31141 ttgtgcaccg gcagcaaagc gtttgctata gcggcgaaac tcgttagccg cctcttccaa
    31201 cgccccggtc agtcgttcgc gttccagagc cggatccagc gttgatgcct gatacacctg
    31261 ttccattaaa ggtaacgtgg catcctgcca gccttcggca atcgccacac caggtgctgc
    31321 cggtaatgca cggattcgcg tctggcgata ttgcccaaac aaggcagtca actgcgactg
    31381 agaaagaata gctgccatct gggtggcaag cgtcaccagg aaggattctt cactttcgtc
    31441 atactggcgc aactctcgtt gctgtaccac cagtacacca agcaactggc gacgttgaat
    31501 aattggtacg cctaaaaacg cgcggaaacg ttcttctttt acggagggga tgtatttgaa
    31561 gctggggtgc ttttgcgcat ctgcaaggtt tatcggttcc gccagcctgc caaccaggcc
    31621 gacgatccct tcatcaaacg cgagcgttac agtgcgaccg cgtggttttt tcagtccccg
    31681 ggtcgccatc aggtagtaac aacgtcgatc atgatcggcc aggtagaccg aacagacctc
    31741 ggtatccatc gcaagacaga tgtcggtaac cagaatattt aacgcctcat tcaggcgtgg
    31801 tgcgctggct accttttcga ctatttcgcg caggcgagtg agcataattg gcgtgactta
    31861 acctctttta cgtcgataag cagatgcgtt ttgtggtttt ggcgtatttt cctgcagtga
    31921 catcaccaca ctcgcgaact ctttcattac cctacggtag acatcacgtt taaatgacac
    31981 cacctgtctg actggatacc agtaacttac ccatcgccag ccgtcaaact ctggtgtact
    32041 gctggtttgc atattgattt ctgcatcgcc gctcaccagc tgcaagagaa accatttttg
    32101 tttttggccg atacaaaccg gcttcgtgtc ccaacgcacc aaacgtttcg gtaatttgta
    32161 gcgcaaccag ttacgcgttg aagcaaggat tcgaacgtct ttgcggctta atcctacttc
    32221 ttcaaacaat tcacggtaca tcgcctgctc tgcggattct ccggggttga ttccgccttg
    32281 cggaaattgc caggagtgct gaccaaatcg ccgggcccac attacctgcc cctggcgatt
    32341 acaaatcacg atacctacgt ttgggcggta gccatcgtca tcaatcaccg gactacctca
    32401 aaataaagct ttatatacga atgattgttt catactccag gaagacggta aaccactctc
    32461 tgcagggcat tacacactaa taacaattga ataactcaca gttatgtgca gagttataaa
    32521 cagaggaagg ggtggatagc cgtttttatt caccttttct gtggatagag ttgtgaagaa
    32581 ctacggaatt actacgggaa aacccggagc attctgaata agacagatac agagatttta
    32641 aaatattcat tatcagtcaa tgcattacat gtgatttgag taataaaaaa gagtaattcg
    32701 tgacccaggt cacacctctc atttacgggt tggcgaaaga tcaaccaatg ccgtatttat
    32761 ccacagaatg tgccactaag ttaagcactg aaccactaaa aactggagtt tcgtcgcacg
    32821 tcaaggctgt aaatggaaac agtagtgaag gtttttcaca gttatcccag ctttctgtgg
    32881 ataacatggt gtaagatcct gtttattttc agtgaccaga tttggaaaac ccgttgcagt
    32941 gttgcgcaac tcgattaccg gcaacctaaa aagcaatata aatcagagaa ttgaacaacg
    33001 catgtggaaa aagttacact gcgaatattc ggcacttaat tgctgtttgt tttttaacca
    33061 aggtatcatg acatgtccca acctcgccca ctgctctctc ctcccgaaac tgaagaacaa
    33121 ttgttagcgc aagcacaaca actttctggt tatacattgg gagaactggc ggcacttgcc
    33181 gggctggtta cgccggagaa tttaaaacgc gataagggct ggattggcgt gttactggag
    33241 atctggctag gtgccagcgc agggagtaaa cctgagcaag attttgctgc tctgggcgtg
    33301 gaacttaaaa ctatccctgt ggatagtctt ggtcgtccgc tggaaacaac attcgtttgt
    33361 gttgccccgt taacgggcaa tagcggggtg acctgggaaa ccagccacgt gcgccacaag
    33421 ctcaaacgcg tgctgtggat accggttgaa ggcgagcgca gcatcccgct ggcaaagcgt
    33481 cgcgtaggat caccgttgct gtggagcccg aatgaagagg aagaccgaca gttacgcgaa
    33541 gactgggaag aattaatgga tatgattgtt ctcggtcaga ttgagcggat caccgctcgt
    33601 cacggggaat atttacagat acgaccgaaa gcagcgaatg cgaaagcgct gaccgaagct
    33661 attggtgccc ggggcgaacg gattctgacg ctgccgcgcg gattttattt gaagaagaat
    33721 ttcaccagtg cgctactggc ccgtcatttt ctgatccagt agccatcgct ttgacctgcc
    33781 gctttccggg catataatta ccgcttcatt tttttggcag ggctttttag atgttatttg
    33841 catggataac cgatcctaac gcctggcttg cgctcggtac actgacgctg ctggagatcg
    33901 ttcttgggat cgacaatatt attttccttt ctctggtggt ggcaaagctt cccacagcac
    33961 aacgtgctca tgcgcgccgt ctggggttgg cgggagccat ggtcatgcgt ctggcgctgc
    34021 tggcatccat cgcctgggtt acgcgcctga cgaatccgct ttttacaata ttcagtcagg
    34081 aaatttccgc ccgtgatttg atcctgcttc tgggtggctt gttccttatc tggaaagcca
    34141 gcaaggaaat ccacgaatcc atcgaaggtg aagaagaagg gctgaaaaca cgcgtttcat
    34201 cattcctcgg cgctatcgtg cagattatgc tgctggatat tatcttcagc ctcgactcgg
    34261 tgattaccgc tgtgggtctg tcagatcacc tgtttattat gatggctgcc gtggtgattg
    34321 ccgtaggcgt gatgatgttc gccgcgcgct cgattggtga ttttgtcgag cgccatcctt
    34381 cggtaaaaat gctggcgctc tctttcctga ttctggtggg ctttaccctg attctggaaa
    34441 gtttcgacat ccacgtaccg aaaggttaca tctacttcgc gatgttcttc tctattgcgg
    34501 ttgaaagcct caacctgatt cgcaacaaaa agaatccgct ctgatactcc gtacgctctc
    34561 ctgcgggagg gcgtactcac tcctgttacc ctcacagttt aagattttcc tgctttcaag
    34621 atatataacg tcggtttata aacagactat tatcataggt gggccaaatg aggataaccg
    34681 gatgaaaaaa tgggcagtaa taatttctgc agtcggactg gcgtttgctg tttccgggtg
    34741 ttccagtgat tacgtcatgg cgaccaaaga tggccgtatg attttgaccg atggaaaacc
    34801 tgaaattgat gatgataccg ggctggtgag ttatcacgat cagcaaggta acgcgatgca
    34861 aattaaccgt gatgatgttt cgcaaattat tgaacgttaa caaataaggt cagcatccgg
    34921 ctggccttaa gatttttctc tttccctttt cccttccctc tgccattttt atattcctta
    34981 tgtcgtgatt ataaaaagga aacggctatg caatatcacc gtatacccca cagttcgctg
    35041 gaagtcagca cgctggggct tggcacgatg acgtttggtg aacagaacag cgaagccgac
    35101 gcccacgcac aactcgacta tgccgtcgct cagggcatta accttatcga cgttgccgaa
    35161 atgtacccag tacctccgcg ccccgaaact caagggctaa ccgaaaccta cgtcggcaac
    35221 tggctggcga aacatggcag ccgcgaaaag ttaattatcg cctccaaagt gagcggaccg
    35281 tcgcgcaata atgacaaagg catccgcccg gatcaggcgc tggatcggaa gaatatccgc
    35341 gaagcgctgc atgacagcct caagcgtctg cagactgatt acctcgatct ttatcaggtg
    35401 cactggccgc agcgcccaac caactgcttc ggcaaactcg gttatagctg gacagattct
    35461 gcgcctgcgg tttcgctgct ggatacactg gacgcactgg cagagtacca acgcgcggga
    35521 aaaattcgtt atatcggcgt gtcgaacgaa actgcatttg gcgtaatgcg ctacctgcat
    35581 ctggcggaca aacacgatct gccgcgtatt gtcaccattc agaaccccta cagtctgtta
    35641 aaccgcagtt ttgaagtagg tctggcagaa gtcagccagt atgaaggggt cgaactgctg
    35701 gcctattcgt gcctgggttt cggcacgctg accgggaaat atctcaacgg tgcaaaaccc
    35761 gctggcgcac gtaatacgct ctttagtcgg ttcacccgct atagcggtga gcaaacgcaa
    35821 aaagccgtcg cggcgtatgt tgatatagcc agacgtcatg gcctggatcc tgcacagatg
    35881 gcgctcgctt ttgtacgccg tcaaccgttt gttgccagca ctctgctggg cgcaaccacg
    35941 atggagcagc tgaaaactaa cgtcgaaagt ttgcatctgg agttaagcga agacgtatta
    36001 gcagaaattg aagcggtgca tcaggtttac acttatccgg caccataaga ttaacgccgg
    36061 ttaaaaccgg cgttaaatat taataacggc gctgccagat ccacagcgcc gttattgcca
    36121 gcgcaaacag cgcaccgaag ccaatgccaa tgggcacgac cgggatgcct accattaccg
    36181 ccagcgagta aatgcccagc atcaacaaca tggcgctgtt ttcgccaagg ttttgtactg
    36241 caatcgcatt ccccgccccg acgctttttt taccccgttc ctgtagcaac gcattgagcg
    36301 gaacgacaaa aaagcccccc agcacgccaa tcagcatcaa caaggcatag gctgacagca
    36361 gctcgtgttg cagggagaaa atcagtacca ccacgccaat caaaatcccg gctggcatac
    36421 agcgtgacac ggtttccagc gtaactaact tcgctgccgc accagcgcca accacgatac
    36481 caatcgctac catcgcgttg agataggtcg gcgtagcgtt atcggtaatg cccagcgcca
    36541 ccggtaccca cagcaccaac aggaaacgca gcgtgacacc cgctccccag aataaactgg
    36601 tgcccaccag cgaaaaacgc gtttcaccat tgcgccatag cgaggtgcag gcattcagga
    36661 aactgcgggt catgctgatg agattccagg actgccccgg acgcgccgcc gccagtttag
    36721 gaatgtagat attggcaacg accgcaccac cgtaggccag tgcgcatgcg gccagggcga
    36781 ccaggacatg ccagtcagcc agtacaccac cggctacgga accgagcaaa atcgccgcta
    36841 tggtagaagc ttccattaaa ccattagctt tcactaactt actacccgtg gttaattcgc
    36901 cgagaatacc gtatttcgcc ggtgaatagg ctgcagcacc aacacccacc agcgtatagc
    36961 cgagaaacgg attgatacca aagcagatac tggctgcgcc cagcagcttc aggccgttgg
    37021 caaacatcat cacccggcct ttggcgaagc tatccgccac ctgtccgaca aacggcgcaa
    37081 aaagaatgta agcacctaca aacaccattt gcaggatggg ctggctccac tccggataaa
    37141 actgcgcttt cagtaacgcc agagtggcaa acagcagcgc attatcgcca aacgcagaga
    37201 gaaactgcgc cacgataacc gctttcatcc ccttcgacca caacgaagtg ttagtgtgca
    37261 ctgactcact catcgtgttg ttccgcttcg tctacccagc ttttcaacgt gacaaagtca
    37321 ggtttgccgc tgccaagtaa tggcatctgt ttcagatagc gaatatcgcg cggtacagca
    37381 agctccggca cgccgtgctc gcgggcatac tgttgcaact tatcgcgcgt cagttcgtta
    37441 tctgtggtga aaagcaccag tgcctcgcct ttgctggcat cgctcttaat cgcagtggca
    37501 tggactttat ctggcgaaac accaagtgcc agttgttcca ccatttccag cgacaccatt
    37561 tcgcctgcaa ttttggcaaa gcgttttgcg cggccctgaa tctgcacaaa gccctgctcg
    37621 tcgaaacgca caatatcgcc agtgtcatac cagccgcgct ccatttcgcc gcgaacattc
    37681 tcggcggtgg gcacttccag tacacctggc ttctccaccc gcagatagcc gttcattatg
    37741 ttcggccctt tcagttgcag gcgtccgccc tcttcgatac caggaaccga caacaggcgc
    37801 gcatccattc ctggtagaat acgccctacc gtaccgggtt tcgccgccat cggtacgttg
    37861 atagaaacga caggcgcgca ctcggtcaca ccgtagcctt caaggatgcg caggccaaat
    37921 ttatcctgcc aaagctgttt ggtactttct tgtagttttt ctgcgcctgc caccacatag
    37981 cgtagacggt agaagtcata cgggttggcg aaacgcgcgt agtgaccgag aaaagtcgag
    38041 gtaccgaaca acacggtgca actgcggtca tacaccaact ccggcacaat gcggtaatgc
    38101 agcgggcttg gataaaggaa cacctctgca cctgtgagca gtggcgtaaa caggcctacc
    38161 gtcagcccaa aggagtgaaa cagcggtaac gccgacataa agcgatcgtt ggtggtgaag
    38221 tcggcaatcg ttttaatctg ctcgacattc gccagaatgc ttttatggct atggacgacg
    38281 cctttcggat ggccttcaga accggaggta aaaaggatca gcgcctcttc ttccggctgc
    38341 tgttttacct gtgccagacg cggcatcagc aaatgagcga agatccatac tttgtcggca
    38401 gtggtgacat ctgcttttaa atcttccaga tagacccagc gcacctgagt aagttgctcc
    38461 ggcagatgcc agagtttgcc tttatcgaga aactgacggg aagtgaaaat ggttttgatt
    38521 tcagccgccg taatagcact ggtcaaccct tttaccccgg cggtgtagtt catcattgcg
    38581 ggaatgcggc gacgggcgat ggccccaaaa atcactgccg cactgatgcc tgcattgggc
    38641 agcattaagc cgatgcgttc gccttcaaca ctgtattttt caaggatgcg tccaacaaac
    38701 agcgttttcg taagcaattt gcgataggag tccggggtaa agttaacgtc ttcgacacat
    38761 ttcttcccgg ctccgaagcg gtacattgcg ctcagtaaag attcgtacag cgtttcacgc
    38821 gggcgcaccg ccatacgcgc ttccatcatt atttgatgca gcatttcgcc agcgattttg
    38881 cgacggtcac gggcacgcgg cgcatccggc atctccacct gcgttggtgg caaaatatgc
    38941 agagtaattt gcgggaacaa gcgacgttta accagacctt tcaggcggct gaagtgcgta
    39001 agttccgccc cttcaatacg cacaggaata accgttgcac cagacttcgc cgcgacaaat
    39061 cccgcgccat cgtagatttt catcagcgag ccagtggtgg tgatgcgtcc ttcagggaaa
    39121 atcaccactg gtcggccctg ttccaccaga cgtaccagat gtttaatcgc cataggttgc
    39181 gtcgggtcga gaggaacaaa gtcgataaat gatttcagcc aacgcatata ccactgttgg
    39241 cttattgagg tgtaaacggc aaataccgga cgtacgggta aaaacaatgc cagcagtatg
    39301 ccatcaataa aagagacgtg attaggcgtg attagaacgc gctcgccctt cagtgcctgg
    39361 gtgtcacccg taacgcgaac gcgatacaaa acacggcaca aatttcgaaa aaagctaaaa
    39421 agcattccaa ctccctttgc tctgattcag taaaagcgaa tggagggaga ttacacgaga
    39481 taaagaacgc gagcgacagt aaattaggtg cgaaaaaaaa cctgcgcatc cgcgcaggtt
    39541 ggtgcaagag acagggtacg aagagcgtac cgaataatct caccaatcaa tacctctggg
    39601 atcttgattg tggtctgcac gacgactctt cgccagtgag aaaacgcaaa ggaatgaagg
    39661 gaaatgcaac gaggtgtgta aattgtcggt tactgttaca gattgatgac cg
//
LOCUS       DAZGQL010000042        38947 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.042, whole genome shotgun sequence.
ACCESSION   DAZGQL010000042 DAZGQL010000000
VERSION     DAZGQL010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38947
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.042"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            102..1484
                     /gene="yicJ"
                     /locus_tag="ACHKIR_002412"
     CDS             102..1484
                     /gene="yicJ"
                     /locus_tag="ACHKIR_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HFO9990145.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAINYTLLTLLYTIVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSATISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQGKRRCQ
                     QQFTSQEVQN"
     gene            1494..3812
                     /gene="yicI"
                     /locus_tag="ACHKIR_002413"
     CDS             1494..3812
                     /gene="yicI"
                     /locus_tag="ACHKIR_002413"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HFO9990146.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQGNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKHPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHSFLSLPVYVRDNTLLALGNNEQRPDYAW
                     HEGTAFQLFNLQDGHEAVCEMPAADGSVIFTLKATRTGNTITVTGAGEAKNWTLCLRN
                     IVKVNGLQGGSQAESEQGLMVTPQGNALTITL"
     gene            4108..5661
                     /locus_tag="ACHKIR_002414"
     CDS             4108..5661
                     /locus_tag="ACHKIR_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFO9990147.1"
                     /translation="MQMITSRQNRLLRFLLPRREYTTIVTIAGYLNVSEKTIQRDLRL
                     LEQWLGQWRINVEKRAGAGVMLSAENITDLLHLDHLLVAECEEIDGVMNNSRRVKIAS
                     QLLSETPNETSISKLSERYFISGASIVNDLRVIESWLAPLGLSLIRSPSGTHIEGSEG
                     QVRQAMALLINGIINHNEPQGVVYSRLDPGSYKALVHYFGEEEVLFVQSLLLDMENEL
                     SWSLGEPYYVNIFTHILIMMYRNTHGNALSREEDQTRQYDENIFNVASQMIHKIEQRI
                     AHTLPDDEVWFIYQYIISSGVAIDGQKDVSIISHMQASNEARLITWRLITVFSDIVDC
                     DFSEDSALYDGLLVHIKPLINRLNYRIHIRNPLLEDIKAELADVWRLTQYVVNQVFKT
                     WGENAVSEDEVGYLTVHFQAAMERQIARKRVLLVCSTGIGTSHLLKSRILRAFPEWTI
                     VDVISAANLSQVLPDNIELIISTINLPTVTMPVAYVTAFFNDADIKRVTEMVITEKLH
                     HATSRVVEI"
     gene            5688..6158
                     /locus_tag="ACHKIR_002415"
     CDS             5688..6158
                     /locus_tag="ACHKIR_002415"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007370547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFO9990148.1"
                     /translation="MDITKILNTNRVILDMQATNKEEAIEELTNLLKKDGAIDCRETF
                     IKDVWQREAEGSTGFENHIAIPHGKSSAVINTTLAIGRTRQDIPWETLDGSSVRCIIL
                     FAVRLEDQNTTHIRLLSQVAGALADDDIIEQLLVETSPQKIIDLFSQYAQSDVC"
     gene            6176..6490
                     /locus_tag="ACHKIR_002416"
     CDS             6176..6490
                     /locus_tag="ACHKIR_002416"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001028073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFO9990149.1"
                     /translation="MNIVCVAACTAGIAHTYIAREKLIKGAKALGHTIKVETQGTIGT
                     ENELQAADISAADVVILAVDVKIKGEERFTNKRIVRVKTEIVIKSPVQFLEKVEKSLG
                     NK"
     gene            6515..7606
                     /locus_tag="ACHKIR_002417"
     CDS             6515..7606
                     /locus_tag="ACHKIR_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFO9990150.1"
                     /translation="MKENKIPVSQEIKKHLLTGISWMIPLIVAAGICIALGQVLGGTD
                     VGEKTGTIPWMLNQIGGWGMGLIVPLISAAIAYSIADRPGFAPGLIVGFLCGQIHTGF
                     IGGMLGGFLVGYTILLLKRYIRLPQSMQGLMPIMVLPVLSTIIGGLLMMTLIGKPIAW
                     LQEALIHLLESMQGGSRFLMGAILGAMATFDFGGPVNKTMSLFSDGLLVSGVYGPEAV
                     KFVGSIIPPFGITLSFLLTRHKYTRAEREALKAAFPMGICMITEGVIPIAARDLLRVV
                     GSCVVASAVAGGLIMVWGVESPVPHGGMFVVPLFTHPLLFCLALGIGTVICGVMLSLW
                     KKPVTERDEEFDELSDQKLKDEEITFTLE"
     gene            7618..8469
                     /locus_tag="ACHKIR_002418"
     CDS             7618..8469
                     /locus_tag="ACHKIR_002418"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFO9990151.1"
                     /translation="MFADMKSMVMKAWHEHYALLAINCMNLESAHAAIRVAEKNRAPI
                     ILNLYQGHLAHFPAPVAAAVVKTLAESASVPVALALDHGKNPDCIRQAFRAGFSGLMI
                     DASAFPLEENVRQTRAVVELAASVQLCVEGELGHLADAPRYDQAANADLMTQPTDVET
                     FIAQTGIDLLAVSVGTAHGMYAPGVVPAIDFQRLAEISRCSSVPLALHGGSGTPFDQL
                     QLCTTLGVAKINVGAAIFERGKSALLHTLCHDISIELVDALKAMELAFEEAITPYLQA
                     SGAIGKA"
     gene            8550..9410
                     /locus_tag="ACHKIR_002419"
     CDS             8550..9410
                     /locus_tag="ACHKIR_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFO9990152.1"
                     /translation="MLVSMKELLQPTRQHGFAIGAFNVADNCFLRAVVDEAEATNTPT
                     IIAIHPSEHDFVGDAFFAHVREITQRSPVPFVLHLDHGASVEHVLRAIRCGFTSVMID
                     GSLLPYEENVALTAEVVRLAHAVGVSVEGELGTIGQTGTSVEGGVSQVTYTDPAQAAD
                     FIARTGADTLAVAIGTAHGIYPKGMQPKLQMDILRDIAGRIDIPLVLHGGSANPDAEI
                     AESVTLGVGKINISSDMKYAYFQKVREILAKESWWDPNVIYPDAINAAREVIRHKMKL
                     FGSLGKASLY"
     gene            9458..10390
                     /locus_tag="ACHKIR_002420"
     CDS             9458..10390
                     /locus_tag="ACHKIR_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFO9990153.1"
                     /translation="MHYLGLDIGGTKIAAVVMDAHGWEIRRYRCPTQKSTYQQFVSCV
                     VALIEQIRRDVQRPMLTGIALPGSISPLTGLIKNANIQVINGHALQADLQQLLGQPVV
                     IANDGNCFALSEACDGAGQDYDVVFGITLGTGCGGGIAIKQRPFIGAWGNAAECGHIT
                     LPGYTEQEDGPSVSCYCGKHNCVESFVSGSGFSERYQQMTGNLLTPAAIVTLAQRGDA
                     CAMQQVARFRQQLARTLATIVNVVDPGVIVIGGGLSNVELLITDLNAEVAPLVFTDQF
                     TTPIVKAQHGDSSGMRGAAWLAMRNGEANETFTN"
     gene            10371..11051
                     /locus_tag="ACHKIR_002421"
     CDS             10371..11051
                     /locus_tag="ACHKIR_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HFO9990154.1"
                     /translation="MKRSQINYVIDKAHAIAQTFRVCLPEFAYFTVDDWLQRERDNWQ
                     EVVDLQLGWDITDFGRGDFNQTGLTLLTMRNGALGSAAYPKPYAEKMLQIQQDQQTPW
                     HFHTHKMEDILNRGGGDLCMQLAWANEANLCDDQRVITVSVDGQRRTMKPGETLVLKP
                     GQGICLPPRLYHRFWAEKAFVLGWEISMVNDDQHDNYFLEPGGRFPAIEEDEPVKWLL
                     CSEYGILR"
     gene            complement(11095..12804)
                     /gene="yicH"
                     /locus_tag="ACHKIR_002422"
     CDS             complement(11095..12804)
                     /gene="yicH"
                     /locus_tag="ACHKIR_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFO9990155.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGSWLRDGKTLLLDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANVPLKPTVTGQLHAVNAAKQQVTQTMNAGVVSSGEVTSTE
                     PVQ"
     gene            complement(12925..14316)
                     /gene="xanP"
                     /locus_tag="ACHKIR_002423"
     CDS             complement(12925..14316)
                     /gene="xanP"
                     /locus_tag="ACHKIR_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HFO9990156.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            14595..15800
                     /gene="gltS"
                     /locus_tag="ACHKIR_002424"
     CDS             14595..15800
                     /gene="gltS"
                     /locus_tag="ACHKIR_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFO9990157.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSMMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            complement(15834..17915)
                     /gene="recG"
                     /locus_tag="ACHKIR_002425"
     CDS             complement(15834..17915)
                     /gene="recG"
                     /locus_tag="ACHKIR_002425"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFO9990158.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFNAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDALKNKLLAALPFKPTGAQARV
                     VAEIERDMALDAPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLAQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(17921..18610)
                     /gene="trmH"
                     /locus_tag="ACHKIR_002426"
     CDS             complement(17921..18610)
                     /gene="trmH"
                     /locus_tag="ACHKIR_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0141100 - tRNA
                     (guanine(18)-2'-O)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HFO9990159.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADRDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            complement(18617..20725)
                     /gene="spoT"
                     /locus_tag="ACHKIR_002427"
     CDS             complement(18617..20725)
                     /gene="spoT"
                     /locus_tag="ACHKIR_002427"
                     /EC_number="2.7.6.5"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HFO9990160.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            complement(20744..21019)
                     /gene="rpoZ"
                     /locus_tag="ACHKIR_002428"
     CDS             complement(20744..21019)
                     /gene="rpoZ"
                     /locus_tag="ACHKIR_002428"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFO9990161.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            complement(21074..21697)
                     /gene="gmk"
                     /locus_tag="ACHKIR_002429"
     CDS             complement(21074..21697)
                     /gene="gmk"
                     /locus_tag="ACHKIR_002429"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFO9990162.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            21955..23640
                     /gene="ligB"
                     /locus_tag="ACHKIR_002430"
     CDS             21955..23640
                     /gene="ligB"
                     /locus_tag="ACHKIR_002430"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HFO9990163.1"
                     /translation="MKVWMAILISILCWQSSAWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGIYDQLSARLTQWQRCFGEETHRDAMMPPLNGAVIHPVAHTGVR
                     KMADKIALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVR
                     LISAVPQTVSGPLANSTLQGEIFLKREGHIQKQMGGINARAKVAGLMMRQDDSDTLNS
                     LGVFVWAWPDGPQLMTDRLKELATAGFTLTQTYTRAVKNADEVARVRNAWWKTKLPFV
                     TDGVVVRAAKEPESRHWLPGQAEWLVAWKYQPVARVAEVKAIQFAVGKSGKISVVASL
                     APVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPT
                     PPENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRSLHQTHRFEHIF
                     SWLLLTPEQLQNTPGIAKSKSAQLWHQFNLARKQPFTRWVMAMGIPLTRAALNASDER
                     SWSQLLLSTEQFWQQLPGTGSGRVRQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            complement(23637..24254)
                     /gene="yicG"
                     /locus_tag="ACHKIR_002431"
     CDS             complement(23637..24254)
                     /gene="yicG"
                     /locus_tag="ACHKIR_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HFO9990164.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(24546..25370)
                     /gene="dinD"
                     /locus_tag="ACHKIR_002432"
     CDS             complement(24546..25370)
                     /gene="dinD"
                     /locus_tag="ACHKIR_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HFO9990165.1"
                     /translation="MNEHHQPFEEIKLINANGAEQWSARQLGKLLGYSEYRHFIPVLT
                     RAKEACLNSGHTIDDHFEEILDMVKIGSNAKRALKDIVLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFRQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAI
                     DFAIFQNHGYRGLYGGLDQKAIHQRKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGRKVRQTIQELGGTMPEELPTPQVNIKQLENSVKITEKK"
     gene            complement(25592..26455)
                     /gene="yicC"
                     /locus_tag="ACHKIR_002433"
     CDS             complement(25592..26455)
                     /gene="yicC"
                     /locus_tag="ACHKIR_002433"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFO9990166.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            26582..27298
                     /gene="rph"
                     /locus_tag="ACHKIR_002434"
     CDS             26582..27298
                     /gene="rph"
                     /locus_tag="ACHKIR_002434"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFO9990167.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAVCDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            27364..28005
                     /gene="pyrE"
                     /locus_tag="ACHKIR_002435"
     CDS             27364..28005
                     /gene="pyrE"
                     /locus_tag="ACHKIR_002435"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFO9990168.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(28042..28638)
                     /gene="slmA"
                     /locus_tag="ACHKIR_002436"
     CDS             complement(28042..28638)
                     /gene="slmA"
                     /locus_tag="ACHKIR_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HFO9990169.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QLLAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(28745..29203)
                     /gene="dut"
                     /locus_tag="ACHKIR_002437"
     CDS             complement(28745..29203)
                     /gene="dut"
                     /locus_tag="ACHKIR_002437"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFO9990170.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            complement(29181..30401)
                     /gene="coaBC"
                     /locus_tag="ACHKIR_002438"
     CDS             complement(29181..30401)
                     /gene="coaBC"
                     /locus_tag="ACHKIR_002438"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HFO9990171.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRERGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            30573..31241
                     /gene="radC"
                     /locus_tag="ACHKIR_002439"
     CDS             30573..31241
                     /gene="radC"
                     /locus_tag="ACHKIR_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFO9990172.1"
                     /translation="MKNNSQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            31458..31694
                     /gene="rpmB"
                     /locus_tag="ACHKIR_002440"
     CDS             31458..31694
                     /gene="rpmB"
                     /locus_tag="ACHKIR_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFO9990173.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            31715..31882
                     /gene="rpmG"
                     /locus_tag="ACHKIR_002441"
     CDS             31715..31882
                     /gene="rpmG"
                     /locus_tag="ACHKIR_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFO9990174.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            31980..32789
                     /gene="mutM"
                     /locus_tag="ACHKIR_002442"
     CDS             31980..32789
                     /gene="mutM"
                     /locus_tag="ACHKIR_002442"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HFO9990175.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCEKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGESCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(32828..33307)
                     /gene="coaD"
                     /locus_tag="ACHKIR_002443"
     CDS             complement(32828..33307)
                     /gene="coaD"
                     /locus_tag="ACHKIR_002443"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFO9990176.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(33315..34592)
                     /gene="waaA"
                     /locus_tag="ACHKIR_002444"
     CDS             complement(33315..34592)
                     /gene="waaA"
                     /locus_tag="ACHKIR_002444"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HFO9990177.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATSLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            35005..36063
                     /gene="rfaQ"
                     /locus_tag="ACHKIR_002445"
     CDS             35005..36063
                     /gene="rfaQ"
                     /locus_tag="ACHKIR_002445"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HFO9990178.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGVDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            36060..37184
                     /gene="waaG"
                     /locus_tag="ACHKIR_002446"
     CDS             36060..37184
                     /gene="waaG"
                     /locus_tag="ACHKIR_002446"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFO9990179.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQFSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            37177..37974
                     /gene="rfaP"
                     /locus_tag="ACHKIR_002447"
     CDS             37177..37974
                     /gene="rfaP"
                     /locus_tag="ACHKIR_002447"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HFO9990180.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGI
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKKEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFVASLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            37990..>38947
                     /gene="waaO"
                     /locus_tag="ACHKIR_002448"
                     /gene_synonym="rfaI"
     CDS             37990..>38947
                     /gene="waaO"
                     /locus_tag="ACHKIR_002448"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008918 - lipopolysaccharide
                     3-alpha-galactosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HFO9990181.1"
                     /translation="MSAHYFNPQEMINKTIIFDERPAASVASSFHVAYGIDKNFLFGC
                     GVSITSVLLHNNDVSFVFHVFIDDIPEADIQRLAQLAKSYRTCIQIHLVNCERLKALP
                     TTKNWSIAMYFRFVIADYFIDQQDKILYLDADIACQGNLKPLITMDLANNVAAVVTER
                     DANWWSLRGQSLQCNELEKGYFNSGVLLINTLAWAQESVSAKAMSMLADKAIVSRLTY
                     MDQDILNLILLGKVKLIEAKYNTQFSLNYELKKSFVCPINDETVLIHYVGPTKPWHYW
                     AGYPSAQPFIKAKEASPWKNEPLMRPVNSNYARYCAKHNFKQN"
BASE COUNT         9352 a         9447 c        10353 g         9795 t
ORIGIN      
        1 tcataaatta ataaccagat atcggaatat tcgctctcgc agggatggtt tacaaaatgc
       61 gttatcggtc tcagcacccc tatagcatca aggaaaagca gatgaagagt gaagtgttgt
      121 ccgttaaaga gaaaattggt tatggcatgg gagacgccgc cagccacatt attttcgata
      181 acgtaatgtt atatatgatg ttcttttata ccgatatttt tggcattcct gccggatttg
      241 tcggaaccat gtttttggtc gctcgtgcac tggatgcgat ttccgatcct tgcatggggt
      301 tgttggccga tcgaacgcgc tctcgctggg gtaaatttcg tccgtgggta ctgtttggcg
      361 cactgccatt tgggatcgtc tgtgtactgg cctatagcac gccagatctc agtatgaacg
      421 gaaaaatgat ttatgcagca attaattaca ccctacttac cttactttat accatcgtta
      481 atatccctta ctgcgcattg ggtggtgtaa tcaccaatga cccgactcag cgtatctcgc
      541 tgcaatcctg gcgttttgtg ctggcgaccg cgggaggcat gctctctact gtgctgatga
      601 tgccgctggt taatttaatt ggtggtgata ataaaccact cggtttccag ggcggtatcg
      661 cggtcctttc cgtggtggca ttcatgatgc tggcattctg tttcttcacc actaaagaac
      721 gcgttgaagc accacctaca acgacgtcta tgcgggaaga tttacgtgat atctggcaaa
      781 acgaccagtg gcggattgtc ggtttactaa ccattttcaa tattctggcg gtgtgcgtac
      841 gcggtggagc gatgatgtat tacgtcacat ggattttggg tacgccggaa gtgtttgtcg
      901 cttttctcac cacttattgc gtgggtaacc tgattggttc cgcactggca aaaccgctga
      961 ccgactggaa atgtaaagtc actatcttct ggtggacgaa cgccctgctg gcagtgatta
     1021 gcctcgcgat gttctttgtt cccatgcagg ccagcatcac tatgtttgtc ttcatcttcg
     1081 tgattggtgt attgcatcaa ctggtgacac ctatccagtg ggtaatgatg tccgataccg
     1141 tcgactacgg cgagtggtgc aatggtaaac gcctgaccgg gatcagtttt gctggcacgc
     1201 tgtttgtgct caagctgggg ttggccttcg gcggcgctct tatcggctgg atgctggctt
     1261 atggcggata tgatgcggca gaaaaagcgc agaacagcgc cacgattagc atcattatcg
     1321 cgctattcac gattgttccg gcgatctgtt acttgctgag cgcgattatc gctaaacgct
     1381 actactcact cacgacgcac aatctgaaaa ccgttatgga acagctggcg cagggcaaac
     1441 gccgttgcca gcaacaattc acctctcaag aagtgcagaa ctaaggaacg gcaatgaaaa
     1501 ttagcgacgg aaactggttg attcaacctg gcctcaattt gattcacccg cttcaggtgt
     1561 tcgaggttga acagcagggt aatgaaatgg tggtctatgc tgccccccgt gatgtgcgtg
     1621 aacgtacctg gcagcttgat acacctttat ttacgctgcg ctttttctcc ccacaggaag
     1681 gtattgttgg tgtacggatt gagcattttc agggggcgct gaataacggt cctcattatc
     1741 cgctcaatat tttgcaggat gtgaaggtca caatcgaaaa cacagaacgt tatgctgagt
     1801 ttaaaagtgg caacttaagc gcgcgtgtca gcaaaggtga gttctggtca ctggattttc
     1861 tgcgcaacgg cgaacgtatt accggtagtc aggtgaaaaa taatggctac gtgcaggaca
     1921 cgaataatca acgaaattat atgtttgagc ggctggatct tggcgttggc gaaacagttt
     1981 acggtctggg agagcgcttt actgccctgg tgcgcaatgg ccagacggta gagacctgga
     2041 accgggacgg cggcacaagt actgaacagg cgtataaaaa tatcccgttc tatatgacta
     2101 accgtggtta tggggtactg gtcaatcatc ctcaatgcgt ctcttttgaa gtgggatcgg
     2161 agaaagtctc caaagtgcag ttcagcgttg agagtgaata tctcgaatac tttgttatcg
     2221 acggcccgac gccgaaagcg gtacttgatc gttatacccg ttttactggt cgtccggcgc
     2281 tgccgcccgc gtggtccttc ggcctgtggc taaccacttc atttaccacc aactacgacg
     2341 aagcgacggt aaacagcttt atcgatggta tggcggaacg caatctgccg ctgcatgttt
     2401 tccactttga ctgtttctgg atgaaagcct tccagtggtg cgattttgag tgggacccgc
     2461 tgactttccc ggacccggaa gggatgatcc gccgcctgaa agcgaaaggg ctaaaaatct
     2521 gcgtctggat taacccctat attgggcaaa aatcccctgt ctttaaagag ttacaagaga
     2581 aaggctattt actcaaacac ccggacggtt cgttatggca gtgggataaa tggcagccag
     2641 gtctggcaat ttatgacttt accaatccgg atgcctgcaa atggtacgcc gacaaactga
     2701 aaggtctggt cgcgatgggc gttgattgct ttaagaccga ctttggcgaa cgtattccaa
     2761 ccgatgttca atggtttgac ggttccgatc cgcagaaaat gcataaccat tatgcgtaca
     2821 tctacaacga actggtgtgg aacgtgctca aggacaccgt tggtgaggaa gaagctgtct
     2881 tgtttgcccg ctcggcttcc gtcggtgcgc agaaattccc ggtacactgg ggtggcgact
     2941 gttacgctaa ctacgaatca atggcggaaa gcctgcgcgg tggtttgtct attggccttt
     3001 caggttttgg cttctggagc cacgatatcg gcggctttga gaataccgcg ccggcgcatg
     3061 tttacaaacg ctggtgcgcg tttggtttgc tctccagcca tagccgttta catggcagca
     3121 aatcttatcg tgtgccgtgg gcctacgatg atgagtcctg tgatgtggtg cgcttcttca
     3181 cgcaactgaa atgccgcatg atgccgtatc tgtatcgtga agctgctcgt gcgaacgcgc
     3241 ggggtacgcc gatgatgcgg gccatgatga tggagtttcc ggacgatccg gcttgtgatt
     3301 accttgaccg tcaatacatg ttaggcgaca acgtgatggt tgcgcctgtg ttcactgaag
     3361 cgggcgatgt gcagttctac ttgcctgaag gtcgctggac acacctgtgg cacaacgatg
     3421 aactcgatgg tagtcgctgg cataaacagc agcacagctt cctgagtctg cccgtttatg
     3481 tgcgtgataa caccctactg gcgctgggca acaacgagca acgtcccgat tacgcgtggc
     3541 acgaaggcac ggcattccag ctctttaatc tacaagacgg gcatgaagcc gtctgtgaaa
     3601 tgcccgctgc tgacggttcg gtgatcttta ctctaaaagc aacacgtact ggtaacacga
     3661 ttactgtgac tggtgcgggc gaggcgaaga actggacact gtgcttgcgc aatattgtga
     3721 aagtaaatgg tctgcaaggc ggttcgcagg ctgaaagtga gcaggggctg atggtgacgc
     3781 ctcaagggaa tgcgctgaca attacgttgt aatctattga tggaatgccg ggtacgaatg
     3841 gttttgtgtc gtatccggta aataatctca catctggtaa taaatatttc ttccatcgta
     3901 tttgcgagca tgatcacgat tattcatcgc gaaaatgtga tctgtgctga aaaatatccc
     3961 ttaaccgcct tgctttgttt tatcactgtc cggattacgg acagatttta gtattgttgt
     4021 gaaattattc tgctttatat ttaccctcag agatggcaac tgaagctctt cctcatgagg
     4081 cttcttaatc tgtgataagg gtaaaaaatg caaatgatta cctcgcgaca aaacagatta
     4141 ttacgattcc ttttacctcg aagggaatat acgactattg ttacgattgc cggctattta
     4201 aatgtttcgg aaaaaaccat tcaacgtgat ttacgtttac ttgagcaatg gctgggacaa
     4261 tggagaataa atgttgagaa gcgtgctggc gcgggtgtga tgttaagcgc ggagaatatt
     4321 actgatttgc tgcatcttga tcatttgttg gtggcagaat gtgaagagat tgatggtgta
     4381 atgaataatt ccaggcgcgt taaaatagcg tcgcagttat taagtgaaac accgaatgaa
     4441 acgtcgatca gtaaattgtc agaacgctac tttatcagcg gagcctctat tgttaatgac
     4501 ctgagagtaa ttgagtcctg gcttgcgccg ttggggttat cgttgatccg cagtccaagt
     4561 ggtacgcata ttgaaggtag tgaagggcaa gtccgacagg caatggcatt actgattaac
     4621 ggcattatta accataatga gccgcaaggt gtcgtgtatt cacgtctgga tcccggaagc
     4681 tataaagcat tagtccatta ttttggagaa gaagaggtgt tatttgtcca gtcattgtta
     4741 ctggatatgg aaaatgaatt aagttggtct ttgggagaac cttattacgt taacattttt
     4801 actcacatcc ttattatgat gtatcgcaac acgcacggga atgcgttatc aagagaagaa
     4861 gatcaaacca ggcaatatga tgaaaatatc tttaatgttg ccagtcagat gattcataag
     4921 atagaacaac gaattgcaca tacattgccc gatgatgaag tctggtttat ttatcaatat
     4981 atcatttcat caggtgtggc gattgatgga caaaaagatg ttagcattat ttcacatatg
     5041 caggccagca atgaagcgcg tctgattacc tggcgtttaa ttacggtatt cagtgacatc
     5101 gtggactgcg attttagtga agacagcgca ttatatgatg gcttattggt gcatattaaa
     5161 ccgctgatta accgactaaa ttatcgtatt catatccgta atccattgtt ggaagatatt
     5221 aaagcagaac tagcggatgt ctggcggttg acgcaatatg tggtgaatca ggtatttaaa
     5281 acctggggtg agaatgcagt gagcgaggat gaagtaggtt acctgaccgt tcattttcag
     5341 gctgcgatgg agcggcaaat tgcccgtaaa cgcgtattac tggtctgttc aaccggaatc
     5401 ggaacttcgc atctactgaa aagccgtatt ctgcgagcat ttcctgaatg gacgattgtt
     5461 gatgttattt cagcagcgaa tttatcacag gttttgcctg acaatatcga actgattatt
     5521 tcgaccatta atttgcctac agtcactatg ccggtcgctt atgttaccgc tttttttaat
     5581 gatgccgata ttaagcgggt cactgaaatg gtgattacgg aaaaattaca tcatgcgaca
     5641 tctcgggtcg ttgaaattta atgtcagaat tttatgaggt atgacgaatg gacataacaa
     5701 aaatcctgaa tacgaatcgc gttattttag atatgcaagc gacgaataaa gaggaggcga
     5761 ttgaagaatt aactaatctt ctcaaaaaag atggtgcgat cgattgtcgg gaaaccttta
     5821 ttaaagatgt ctggcagcgc gaagcggaag gctcgactgg ttttgaaaat catattgcga
     5881 tcccgcatgg taaatcttca gctgtgatta atactacgct ggcaattggc cgtacccggc
     5941 aggatattcc ctgggaaacg ctggatggca gtagcgtgcg ctgtattatt ttatttgcgg
     6001 tacgtcttga agatcaaaat accacccata tccgtttact ttcccaggtc gcgggcgcgc
     6061 tggcggatga tgacatcata gaacaattgt tagttgagac gagtccgcaa aaaatcattg
     6121 atctctttag tcagtatgca caatccgatg tctgctaaat aacagggagt atgtcatgaa
     6181 tattgtctgt gttgccgcct gtacggcggg tattgctcat acctatattg ctcgtgaaaa
     6241 gttaatcaaa ggagcaaaag ctctgggtca taccattaaa gttgaaaccc agggaaccat
     6301 tggtacagaa aatgaattac aggcggcaga tatctctgct gctgacgtcg tgattcttgc
     6361 tgtagatgtg aaaataaaag gagaagaacg cttcaccaat aaacgtattg tgcgagtaaa
     6421 gacagagatc gtcattaaat cgcccgttca atttcttgaa aaggtcgaaa aatcactggg
     6481 aaacaaatga tccttatact ggagggatct taatatgaaa gaaaataaaa ttccggtttc
     6541 acaggaaatt aaaaaacatt tattaaccgg catctcgtgg atgatacccc ttattgtggc
     6601 cgccgggata tgtattgcgt tggggcaagt tcttggtggt acagatgttg gtgaaaaaac
     6661 aggaacaatc ccgtggatgc ttaatcagat tggcggctgg ggaatggggc tgattgtgcc
     6721 gctgattagc gcggcaattg cctattctat tgccgatcgt cccggatttg ccccgggcct
     6781 gattgtcggc tttctctgtg ggcaaatcca taccggattt attggcggta tgttgggtgg
     6841 tttcctggtg ggttacacca ttttgctact taaacgctat atccgcctgc cgcagtcgat
     6901 gcaaggactc atgccaatca tggtgttgcc ggtattaagc accattatcg gtgggctgtt
     6961 aatgatgacg ctgattggta aacccatcgc ctggttacaa gaagcactga ttcatttgct
     7021 cgagtcgatg cagggcggat cgcgcttcct gatgggggcc atacttggcg cgatggcaac
     7081 cttcgatttc ggtggtccgg tcaacaaaac catgtcgctg ttttccgatg gcttgctggt
     7141 cagtggcgtc tacggaccag aagccgtgaa atttgtcggc tcaatcatcc ctccgtttgg
     7201 catcacactt tcgttcttac tgacacgcca taaatacacc cgtgcggagc gcgaggcgct
     7261 gaaagccgcg tttccgatgg ggatctgcat gatcactgaa ggagtcattc cgatcgccgc
     7321 gcgtgacctg ctgcgagtag tgggatcttg tgtggtggca tcggcggtcg cgggcggctt
     7381 aatcatggtg tggggcgtgg agagtccggt tccgcatggc ggcatgtttg ttgtgcctct
     7441 gttcacccat cctctgctgt tttgcctggc gctgggtata ggcacggtga tttgtggcgt
     7501 gatgctttca ctctggaaga aaccggtaac ggaacgcgac gaagagtttg acgaactgag
     7561 cgatcaaaag ctgaaagatg aagagatcac tttcaccctc gaataaccgg aggccctatg
     7621 tttgccgata tgaaaagtat ggtgatgaaa gcctggcatg aacattacgc tttgctggcg
     7681 atcaactgta tgaatctgga aagtgctcac gcagctatcc gcgtggcgga gaagaatcgc
     7741 gctccgatca ttctcaatct ctatcagggg catctggcgc attttcctgc acccgtcgcg
     7801 gcggcagtgg ttaaaacgct cgcggaatct gcctcagtac cggtcgctct ggcgcttgat
     7861 catggcaaaa atccggactg tattcgtcag gcgtttcgtg ctggtttcag cggattaatg
     7921 attgatgctt ctgcttttcc cctggaggaa aacgtgcgcc agaccagggc ggtggtggaa
     7981 ctcgccgcca gcgtgcagct ctgcgtggaa ggcgaactgg gccatctggc agatgcgccg
     8041 cgttacgacc aggccgccaa tgcagacctg atgacgcaac ccactgatgt ggaaacgttt
     8101 atcgcgcaga caggcattga tctactggcc gtctcggtag gcacggcgca cggcatgtac
     8161 gcgcctggtg ttgtacctgc tattgatttt cagcgtctgg cggagatttc gcgctgctcg
     8221 agcgtgcctc tggcattaca tggcggtagc ggtacgccgt tcgatcagct ccagctctgt
     8281 acgacgctgg gggtagcaaa aatcaacgtg ggggcggcca tttttgaacg ggggaaatcc
     8341 gcgctcctgc ataccctctg tcacgatata tccattgaac tggtggacgc gctgaaagct
     8401 atggaactgg ccttcgaaga agcgattaca ccgtatttac aggcgagcgg cgctatcggc
     8461 aaagcctgat tcattttctg tcatggcctt tatgccagtg caataacgca ccggcagggc
     8521 ggactacacg ctgaaactgg agatttaaca tgttagtttc gatgaaagaa ttactgcaac
     8581 ctacccgcca gcacggcttt gccattggcg catttaatgt ggcggataac tgtttccttc
     8641 gcgcagtcgt ggatgaagca gaagcgacaa atactccgac gattattgcc atccatccca
     8701 gcgaacatga ttttgtgggc gatgcctttt tcgctcacgt tcgcgaaatt acccagcgta
     8761 gtccggtccc gtttgtactg cacctcgatc atggtgcctc tgtagagcat gttttgcgtg
     8821 cgattcgctg cggatttacc tcagtaatga tcgacggttc gctgctgcca tatgaagaaa
     8881 acgtggcgct gaccgcagaa gtggttcgcc tcgctcacgc ggttggagta tcggttgagg
     8941 gtgaactggg aaccatcgga caaacaggaa cgtcggtaga aggtggtgtt tcacaagtga
     9001 cctacacaga tccggcgcag gccgccgatt ttattgctcg tacaggtgcc gacacactgg
     9061 ctgtggcgat tggtacagcg catggcatct atccaaaagg gatgcagccg aagctgcaaa
     9121 tggacatttt gcgcgacatt gccggacgta ttgatattcc gctggtgcta cacggtggtt
     9181 cggcaaaccc ggacgcggag attgctgaat cagttacgct cggcgtcggc aaaatcaata
     9241 tctccagcga tatgaaatac gcctatttcc agaaagtgcg tgaaattctc gctaaagaga
     9301 gctggtggga cccgaacgtc atctatccgg atgcgattaa cgcagcgcgg gaagtcattc
     9361 gccacaaaat gaaactgttc ggttcgctcg gtaaagcatc actgtactaa gccagaaggg
     9421 cggggtgacc cgccttttac gcaacaagga acgagatatg cactatctgg ggttggatat
     9481 tggtgggacc aaaatcgccg ccgtcgtcat ggatgcgcat ggctgggaga ttcgccgtta
     9541 ccgctgcccg acgcaaaagt cgacatatca acaatttgtc tcatgcgttg tggcgcttat
     9601 cgagcagatt aggcgggacg ttcaacgacc gatgctgaca gggatcgcct tacccggcag
     9661 tatctcgcca ctcactggcc tgattaaaaa cgcgaatatt caggtgatta acggtcatgc
     9721 gttacaggct gatttgcagc aattgcttgg gcaaccggtg gtgatagcca atgatggtaa
     9781 ctgtttcgcg ctatcagaag cttgcgacgg tgccgggcaa gattatgacg tggtatttgg
     9841 tattacgctt ggtacgggct gcggcggtgg cattgccatc aagcaacgac cgtttatagg
     9901 ggcctgggga aatgctgccg aatgcggtca tatcacgttg ccaggctata cggagcagga
     9961 agatggtcca tcagtcagtt gctattgcgg caaacacaac tgcgtggagt cgtttgtttc
    10021 cggcagcggt tttagtgaac gctatcaaca gatgactggt aacttgctca ctcctgcggc
    10081 gattgtcacc ctggcacaac gtggtgatgc ttgtgccatg cagcaggtgg cacgttttcg
    10141 ccaacagctt gcccgcacgc tggcaaccat cgttaatgtt gttgaccctg gcgtgattgt
    10201 gatcggcggc gggctttcga atgtggaact gcttatcacc gatctgaacg cagaagtcgc
    10261 tccactggtt ttcaccgatc aattcaccac tcccattgta aaagcacagc acggcgacag
    10321 tagcggaatg cgtggcgctg cctggcttgc tatgcgcaac ggagaagcca atgaaacgtt
    10381 cacaaattaa ttatgttatt gataaagcac atgccattgc gcagaccttc cgcgtttgtt
    10441 tgccagagtt cgcctatttt acggtggatg actggctgca gcgggaacgg gataactggc
    10501 aagaagtggt cgacttacag ctcggttggg atatcaccga ttttggtcgt ggtgatttca
    10561 accaaacggg actaacactg ctaacgatgc gcaacggcgc gctggggtcg gcggcatatc
    10621 caaaaccgta tgctgagaaa atgttgcaga tccaacaaga tcagcagact ccctggcatt
    10681 ttcacaccca caaaatggaa gatatcctca atcggggcgg tggagactta tgcatgcagt
    10741 tggcctgggc caacgaagcc aatctctgtg atgatcaacg agtcattacc gtgagtgttg
    10801 atggtcagcg gcgtaccatg aaacctggtg aaacgctggt gctaaagccg gggcagggca
    10861 tttgcctgcc gccgcgtctg tatcatcgct tctgggcgga aaaagcgttt gttctcggct
    10921 gggaaatctc gatggtaaat gatgaccaac acgataatta ttttcttgag ccgggaggac
    10981 gttttccggc gatagaagaa gatgaaccgg tgaaatggtt attgtgcagt gagtatggaa
    11041 ttttgcgcta ataacgggga acgctagcgc tgatatccgc tagcgtcctt atttttactg
    11101 caccggctcc gtcgacgtaa cttcaccact ggaaactacg cccgcattca tggtttgagt
    11161 cacttgctgc tttgcggcat tcacggcatg caattgcccg gtaaccgtag gtttcagcgg
    11221 gacattggcc tgaatatcgc cactggcagt aagctgaata ttgccgtcgc cagtcaacgg
    11281 taattcaggc catccccact gttgcaaaat attcaccggc acaccgcgtc cattcaggct
    11341 gatatgggtc tgacgttgtg gcgtttgtga aacactggcg gtggcttcca gaatgccttt
    11401 ttcagtaaat gcactcagtt cgctgatatt caccgtgctg ctgttggcgg tcagcgccag
    11461 cgatgggcga cgaacatcaa cacgattgaa tgtcgcggcg gcggcattca gattcgccga
    11521 accactccag acgccccatt tatgatcggt aaccagcgtc aggttggcac cgtaaccatc
    11581 gagcgcggtg agctgccacg ggaagtcagg gtcgatatca atgatcagat tgcggctggc
    11641 gctaaatctc ttcagttgca ggctatttaa ccaaccgggt gtcgtttcca tccacaactg
    11701 ttgccagttt tttggcaagg tatattccag cccggcaatt gccgcatcat caaggagtaa
    11761 cgttttcccg tcacgcaacc agctccctga cgttctgacc ataccccctt cccagcggct
    11821 ggtgaactgg cgcagcgcta cgccctgcgg ggaaaattcc gcgtttataa tcgggtcaaa
    11881 taaatgcagc gaaccataaa tgaactcgct agcgttcatc gacagtttgc cttcctgtgt
    11941 ctgccagtca tctttactga aggtcatgtt gcgcaagctg agatcgaggt cggtcaccgc
    12001 ccagtccgga ccttgcaaac gagcgtcaat gacttccagg cgaccaatct gcaacgacgg
    12061 gacagagcgt aatggcgcaa agaagtcggt tagcgatttt tcgctttgta gacggatatc
    12121 cgccatgcgc agattttcca cttgccagct gccgtcggcg ttacgctgcg cgtttccggt
    12181 taatgtcccg cgggcgatgt cggcacccaa gttagtcagc gtaacgcgat cgttatcaat
    12241 actgccttca atcagtacat tggtggcagg aacatcattg agcgaaagcg atccggcact
    12301 aaactgaatc tgcgccttcg tacccagcac tttaccggct tctggtgacc acggaaccac
    12361 gccaccattt acccgctgcg cgcttagttt ccattcgcta ttcgggctat taaacgccat
    12421 atcacgcagt tgcagacgat cggctttgaa cggtagcggc gcggtctggt cggtgagatt
    12481 cagcgtcccg ttttccagca agatggtatc gacatggcgt ggttcggtca gttggcgact
    12541 gcttagcgca atgtcgacac ttttggccac cagggtcgcg ggctgaccat cacgaccaaa
    12601 cgtgacgttc tccagcacga tatgagatgg cgcggaaaaa cggtgatcca tcgccccgaa
    12661 ggccagatga tagtcgctat tctcggaaac ccatgcgctg atatgttctg ctccccagcg
    12721 ggtttgcaga agaaaataga ggccagcgat caccactaac agagcgatga gaatgtagag
    12781 aagcagcttc ccaataaatt tcatggtctt ccatcccgtg aaaagcacat aagggagtta
    12841 tgcacgattt acgcgcaatc ctcaaggcgg gaatggtgaa agacgtgaga ctgcggcggt
    12901 aattatctta ccgccgccag tgaattactg tttttctggt gggaaaatca gattcagcac
    12961 gatggcagta ataccgcccg cggcaatccc ggaggagagc aggtttttca gccattcagg
    13021 ggcaaactgc aaaatcagcg gctgctgaga gacgcccaga ccgaccgcca gcgacagcgc
    13081 gataatcaga atcgcccgac ggttcagcgg ctcacgagaa acgatacgca caccggaggc
    13141 ggcgatggtg ccaaacatta caagcgttgc gccgcccaga accggttcag gaatgtgttg
    13201 tacaaaaccg ctcactgccg ggaacagacc cagcacgatc aacatcagcg cgacgacaaa
    13261 accgacatag cggctggcaa caccggttaa ctggatcact ccgttgttct gcccgaagca
    13321 ggagttcggg aaggtgttaa acaccgccga aacaaacgag ttcaggccgt ttgccagcac
    13381 accgcctttc aggcgtttca tgtacagcgg accggacact ggctgttcgg aaacgtcaga
    13441 ggtcgccgtg atatcgccaa tggtttccag cgaagtgatc ataaagacca gcatcagcgg
    13501 cagcagcaga ctccattcaa tgccaagacc gtaatagagc ggcgttggca ccataatcag
    13561 ttcttgtgtc atcggttcgt tgctttctgg caacatgccc ataaaccacg ccagcgcata
    13621 tccggccgcc atcgcaatta ccagtgaggc cacgcgtaag taagggttac gttgacggtt
    13681 aagcaggata attaaggcta aaaccacgcc tgccagcagc agatttttcg gtgcgccgaa
    13741 ggtgttatcg ctcatggctg cgtaaccgcc gccaatggac gttaacccaa cctgaattag
    13801 cgacaggccg ataatcatca ccacaacgcc agaaactagc ggcgtaataa tgcggcgcgc
    13861 cagatgcaga acgcgggaga tcaccatctc agtgcaactt gccagcatca acgtgccgaa
    13921 caaagccgcc atcatggtag gaacatcagc accaccggtt ttcagcgcgg taccgcccat
    13981 aatcagtggg gcaacaaagt tgaagctggt gccctgaata gacaacagcc cggagccaac
    14041 cggaccccag gccttaattt gaataatcga tgccacaccg gaggcaaaca gcgacatact
    14101 aataatgtgt tgcgtgtctt gtgccggtaa acccagcgcc tggcagatta atagcgctgg
    14161 cgtgatcacc gcaacgaaca tcgccagcag atgctgacag gcggcaaaca gagtttgagg
    14221 aagcggcgga cgatcttcaa gacggtaaat cagttcgctg ttttgagtct gcgcaaccgg
    14281 ttgcgcattt tctgactcga gggtggaaac agacatcgaa aacaatcccg ttgtagcaaa
    14341 gcgggcattt tagctgaccg aaagagaaaa gcaaacgttt gccacggcct ctgctaaata
    14401 tttaaatgag ataaatgcac ttttttatat aacttttggt atttttctgc ttaaaatcca
    14461 tgccatttgt tcattaacag ctgagaattt gacccaggaa agcataactc gatacccgtc
    14521 atagttcata agttgccact gcgcgatgaa gtatgacgag tatgaaagag tgatgcggat
    14581 acaaaggagt aactatgttt catctcgata ctttagcaac gcttgttgcc gcaacgctga
    14641 cgttgctgct cgggcgtaag ttggtccatt ccgtctcctt tttgaagaaa tacaccatac
    14701 cggaacctgt tgcgggtggt ttgttggtgg cgctggcgct actggtactg aaaaaaagca
    14761 tgggctggga agtcaacttt gatatgtccc tgcgcgatcc gttaatgctg gcattcttcg
    14821 ccaccattgg cctgaacgcc aacattgcca gtttgcgtgc cggtgggcgt gtggttggca
    14881 tcttcttgat tgtggttgtt ggtctgttgg tgatgcaaaa tgccattggc attggtatgg
    14941 ctagcctgtt agggcttgat ccgctgatgg ggctgttggc cggttctatt acgctttccg
    15001 gcggtcacgg tacgggcgct gcgtggagta aattgttcat tgaacgctat ggcttcacca
    15061 atgcgacgga agtggcgatg gcctgtgcga cgtttggtct ggtgctgggc ggcttgattg
    15121 gcggtccggt agcgcgctat ctggtgaaac actccaccac gccgaacggt attccggatg
    15181 accaggaagt cccgacggcg ttcgaaaagc cggatgtggg ccgcatgatc acctcgctgg
    15241 tgctgattga aactatcgcg ctgattgcta tctgcctgac ggtggggaaa attgttgcgc
    15301 aacttttggc tggcactgct tttgaactgc cgaccttcgt ctgtgtactg tttgttggcg
    15361 tgattctgag caacggtctg tcaatgatgg gcttttaccg cgtctttgag cgtgcggtat
    15421 ccgtgctggg taacgtaagc ctgtcgttgt tcctggcgat ggcgctgatg gggctgaaac
    15481 tatgggagct ggcttcgctg gcgctgccga tgctggcgat tctggtggta cagaccatct
    15541 tcatggcgtt gtatgccatc ttcgttacct ggcgcatgat gggcaaaaac tacgatgcgg
    15601 cagtgctggc tgcgggtcac tgtggttttg gcctcggtgc aacgccaaca gcaatcgcca
    15661 acatgcaggc gatcactgaa cgttttggcc cgtcgcacat ggccttcctg gtagtgccga
    15721 tggtcggtgc gttctttatt gatatcgtta acgcgctggt gattaagctg tatttaatgt
    15781 tgccgatttt tgccggttaa ccgatgaagc ggcggtcttc tcaccgccgc cttttacgca
    15841 ttcgagtaac gttccgtttc tggcatccag cgttctatca gggcttttgc ctgctgtggg
    15901 taacgttcgt gaatatggcg tgccaggcgc tgaacttccg ggatcatcgc ctgatcgcgc
    15961 agtaaatccg ccactttaaa ttcagcatta cccgtctgac gcgtgcctaa caattcgcca
    16021 gggccgcgaa tctccagatc tttttgcgca atcacaaacc cgtcgttgct gtcgcgcagt
    16081 acttgcagac gaatttgcgc cgttttagag agcggcgttt tgtagagcag cacgcagtga
    16141 gaagccaccg caccacgtcc cacgcgcccg cgaagctggt gcaactgcgc cagacccaga
    16201 cgctccgggt tttcgataat catcaggctg gcgttaggca catcaacgcc gacttcaata
    16261 acggtagtgg caaccagcag gtgtaactcg ccttgtttaa atgatgccat gacggcctgc
    16321 ttctcggctg gtttcatccg tccgtgtacc agaccaacat tcaactctgg tagcgccagt
    16381 ttcaactctt cccaggtagc ttccgccgcc tgcgcttcca gtaactctga ctcttcaatc
    16441 aacgtacaaa cccagtatgc ctgacgacct tcagttatgc aggcgtggcg cacgcggtca
    16501 ataatgtcgg tacggcgggt atcaggaata gcgaccgtag tcactggcgt gcggcctggc
    16561 ggcagctcat ctatcaccga ggtatcgaga tcagcatacg cagtcattgc cagcgttcgg
    16621 gggatcggcg tggcggtcat gatcaactga tgaggatgga agccttgctg ctggcctttc
    16681 tcccataacg ccagacgctg atgcacgcca aaacgatgct gttcgtcgat aatcaccagc
    16741 gccaggccgt taaactgcac ctgttcctgg aagatggcgt gtgttccgac aatcatctgc
    16801 acctgaccgc tggcgatggc ttcctgctgt gccagtcgcg ctttaccttt ctgcttaccg
    16861 gcgagccagc ccacttcgat accgagcggt gcaaaccagt tgcggaagtt attcgcgtgc
    16921 tgttcggcga gtaattcggt tggtgccatc aatgccacct gtttgccgtg cgcaatcgca
    16981 cgcagcgcgg cgagggcagc gaccagcgtt ttaccggaac ctacatcgcc ctgtaccagg
    17041 cgcatcatcg gcgcatccag cgccatatcg cgctcgatct ccgccaccac gcgtgcctgt
    17101 gcgcccgttg gcttgaacgg taaggctgcg aggagtttat ttttcagcgc gtcattggcg
    17161 ctaagcggct gggcatggaa acgttgtgca ccagcgcgta acgccaacat gctgaggttg
    17221 tgcgcaagca gttcttccag aatcagacgg cgttgtgctg gatgctgccc ggtttcgaga
    17281 tcgctaagct gtagcgtcgg cggcgggcgg tgcaaagtgc gcaacgcttc cggtagcgtc
    17341 atcattcctt gtgacagttc cggcggcagg agttcttcaa tggcgcaggt gtcgagcaga
    17401 tccagcgcct gatcggttaa tttacgcagc gtggcctgct ttacgccttc cgttgttggg
    17461 taaaccggcg ttagcgtttc ctgcaactct ggcgtactga gatcgccctg cacgcggtat
    17521 tccgggtgga tcatctccgc accatattta ccgcgctttg cttcgccata ggccagcacg
    17581 cggcggcctg tcgccaggct atttttcatt gccgcgttga agttgaaaaa gcgcatggtg
    17641 agaatgccgg aaccgtcgct gatctggcag gtcatcatcc gccgaccgcc gaaggagata
    17701 ttgcagttca gcacttcgcc ttccaccgtg gcataaacgc ccggtagtag ttctccgatg
    17761 gggtagagat gggtgcgatc ttcgtagcgc aggggaaggt gtaagagtag atcctgcacg
    17821 gtatgcaggt tgattttcgc cagcttgtta ctaagcgctg cgccaacgcc cgttagggaa
    17881 ctgagcggga cagcatctaa caggcgacct ttcatggcac ttaccctgca gcctgcatgg
    17941 tagcccacca gtcagcatcg gcttcaattt cgccttgctg attaacgtgg gggtaaggca
    18001 ggcctttgcg ttttgcgact ttcgccagca ccggatagcc gccttcaaac agcaggcgtt
    18061 gttgctctgc ttccggcaac atgctgtttt cacgcaggta catgcctgca ttttgccgct
    18121 ggcgctgggc ttcataaaga atgagggctg aggcaacgga aacattcagc gactgcacca
    18181 tgccgatcat cggtatgatg atgtcccgat ccgccagggc caacgcttcc tgcgtgatgc
    18241 ctgttttctc ctgtcccatc aaaatacagg tcgggcgagt gtaatcaatt tcgcggaaat
    18301 cgacagcgtt atcagaaaga tgggttgcca gaatctgcat gccctggcct ttcagatgag
    18361 cgacggcatc gccaatggtg cggtgcgttt tcacctgtac ccagctgtta ctacccgcag
    18421 ctgccgaagc catggtgcgc atccggctac caggccagac agcatgaact tcatgtacac
    18481 caacggcatc tgcggtacga ataatcgcag aaacgttatg aggtttgtgg acctgctcca
    18541 tgcagacggt cagatcaggc tgccgcctgg cgagcatttc gcagatgcgt gcataacgtg
    18601 ttgggttcat aaaacattaa tttcggtttc gggtgacttt aatcacgtct ggcatcacgc
    18661 ggattttgcg catgatattc gccagatgca cacggtcacg ggcggtcaga cgaataaagg
    18721 cgctgtagac gcgaccatct ttctcttccg tattcaaact ttgaatattc gaggtggtgg
    18781 tgttaattgc cgctgtcagg tttgccagcg caccctgatg attgaacatc tccaccttga
    18841 tttcggtgat gaactcctgc gccgtctctt tatcccattc cacagccata aacttctctg
    18901 gctctttctg gtagccacgg atgttacggc aggattcatg gtggatcacc agacctttac
    18961 cggggctgac gtgggcgata atcgggtcgc caggaatagg gcggcagcat ttcgcaaagg
    19021 tgatcagcac gccatcggca cctttaatgg gcagatgtcc gtgactttgg gttgccggtg
    19081 gaatggaggc gtccccatgt tgcagatttt tcgcgaccac cacgctcatt gcgttaccaa
    19141 ggccgatttc agccagcaga tcgtcaagcg ttgccagctt catgcggtcc agctcgcgct
    19201 gaatattttc ctgcgggatt tcattgagct tgcggctgcc acccaacgca tggttgagca
    19261 gacgacggcc caggcttaca gaatcatcac gcttgaggtt tttcagcaat tgacgaattt
    19321 tggcgcgcgc tttcgagcta acgacaaagt tcagccacgc ggcattcggg cgagcgcccg
    19381 gtgcggtaat aatttcaacg gtttgaccgc tggtaagcgg ctgcgacagc gggtaaggct
    19441 ggcggtcgac gcgtgcgccc acgcaggcat gaccgatatc ggtatgcacc gcataagcga
    19501 agtcgacggg cgttgcaccg gcaggcagtt cgacaatgcg cccttctggt gtgaaaacgt
    19561 aaatctcatc cgggaagaga tcggatttaa cgctctcgat aaattcaaac gagctaccgg
    19621 cactctgttg cagctccagc aggctttgca tccagcgctg ggcgcgtatt tgcgcggtag
    19681 tgctggtttc gccgtgctct ttataagccc agtgcgcggc aacacccatc tccgccatct
    19741 gatccatatc ttcggtacgg atctggacct caaccggcac gccgtgcggg ccaatcatcg
    19801 aggtgtgcaa agactgatag ccgttcgctt ttggaatggc gatatagtct ttcacgcggc
    19861 ccggacgcgg cttgtacagg ctgtgcatct ggcccagcac gcgataacag gtgtcagaat
    19921 cattgacgat cacgcggaaa gcgtagatgt ccatgattga gtgaaaacgc tgctctttga
    19981 gcaccatttt gcagtaaatc gaataaagat gcttttcgcg accactgacg cggcacggta
    20041 ttcccgcttc ctgcaaacgc ccttcgattt cagaaagaat cttctggatc atctctttac
    20101 ggttgccgcg cgcggctttc accacttctt tgattacgcg ataacggttg ggatacagcg
    20161 cctcaaaacc cagctcttcg agttcggttt taatgtggtg gatacctaaa cggtgcgcca
    20221 gcgggctgta aatttcgagg gtttcacggg cgatgcggcg acgtttgtcc gggcgaagtg
    20281 agcccagcgt gcgcatgttg tgggtacggt cggcaagttt gatgagaatg acgcggatat
    20341 cctgcaccat cgccataatc atcttgcgaa agttttcggc ctgcgcctct ttcttatcgc
    20401 ggaacttgag cttatcaagt ttcgacaccc cctctaccag ctcggcgacg cttttaccaa
    20461 aaagctgttc catgtcctgg taggtggcgg gagtatcttc aatcacgtca tgcagcagcg
    20521 ccgccataag cgtttcatag tcgagtttca tctcggccag aatgcaggca accgccaccg
    20581 ggtgcgtgat atagggttca ccgcttgaac gtgtttgccc ctcgtgagca tcacgtgcaa
    20641 cgagatacgc ctgccgcaga cgcttgattt ggtcttccgg caggtaggtt tgaatcagtt
    20701 gattcaggct ttcaaacaga tacaagggcg acccgctttg tgattaacga cgaccttcag
    20761 caatagcggt aacggcttgt aattcagcgg cttcctgctc ttgctgttcc tggcgttcgc
    20821 gaacgtcgag gatctggttg ttgatcagac cttcttcgat ttcgcgcagc gcgattacag
    20881 tggttttatc gttttcttcc ggtaccagcg gatcctttcc gcctacctgc atctgacgag
    20941 cgcgacgcgc ggcgaccagt accaggtcaa aacggttacc aattttctct acagcgtcct
    21001 gaacagttac gcgtgccata cttaaaaagc tccacaggtg aagaaatgac tgggcatgat
    21061 actgaaatca ggttcagtct gccaacaatt tgctgattaa agcgtcatga cgctgctttt
    21121 ggcggctcat gcgcagacgt tcggcgcgaa taatggtctt caaatcggtc aacgcggtat
    21181 cgaagtcatc attcacaatc aggtaatcat attcggcgta atggctcatt tctgcaacag
    21241 cttgcgccat acgctttgca atgacctctt cgctgtcctg accgcgaccg cgtagacggc
    21301 ggtccagttc aattttggac ggcggtaaaa taaagatact ccgcgcgtgc ggcatcttct
    21361 ggcgaatttg ctgcgcaccc tgccagtcga tatcgagaaa aacatcgaca ccggtcgcca
    21421 gtacttgctc aatggcctca cgcgaagtgc catagtaatt accaaaaact tctgcgtgtt
    21481 cgaggaacgc atctctgcta atcatttctt taaattcatc atgattaaca aagaaataat
    21541 gttcaccgtg gacctcacca ggacgcggtt ggcgtgtggt gtgtgaaaca gaaacctggg
    21601 tgtcatacaa cggttgggtt tttaataaag cctgaatcag gctggattta cccgcgccac
    21661 tgggggcaga aacaatataa agcgtgcctt gagccatgag tatctttcgt atgtgattag
    21721 cgaaataaag cctacatacg agcttattat acacggcgcg gccatgtgac gtagcccttg
    21781 ctcacacttt ttgcgcaaag ttgttgcgtt ttgcgtagct gcttccgcat ttacctgtca
    21841 ttcactgcaa taaagacgat tctcaggaag cgtcctcgca gactccgttt ttgccactcg
    21901 gctttgcttt catcacgttg tatatctgcc tgactatcta cagcggaggt gatgatgaaa
    21961 gtatggatgg cgatattaat aagtatcttg tgctggcaat catcggcgtg ggcagtctgt
    22021 ccggcctggt cgccagccag agcacaggaa gaaatttccc gcctgcaaca gcaaataaaa
    22081 cagtgggacg atgactactg gaaagaaggg aaaagtgagg tggaagacgg tatttacgat
    22141 cagttaagtg cccgtctcac gcagtggcaa cgctgttttg gtgaagagac gcatcgcgac
    22201 gctatgatgc cgcctttaaa cggtgcggtt atacatccgg ttgcacatac gggtgtacgc
    22261 aaaatggcgg ataaaatcgc attaagtctg tggatgcgcg aacgtagcga tctttgggtg
    22321 cagccaaaag ttgatggcgt tgcggtaacc ctggtttatc gggacgggaa actgaacaaa
    22381 gcaatcagtc gcggcaatgg cctgaaaggc gaggactgga cgcagaaagt tcgcttaatt
    22441 tccgctgtgc cgcaaaccgt tagcgggcct ttagccaaca gtacgcttca gggggaaata
    22501 tttctcaaac gcgaggggca tatccaaaaa caaatggggg gaataaatgc ccgcgcaaaa
    22561 gttgctggtc tgatgatgcg ccaggacgat agcgacacgc tgaattctct gggcgttttt
    22621 gtctgggcat ggccggatgg accgcagtta atgaccgatc gtttaaaaga gctggctacc
    22681 gctggtttta ctctaacgca gacgtatacc cgtgcggtga aaaatgctga tgaagttgcg
    22741 cgcgtacgca atgcgtggtg gaaaacgaaa ttacccttcg tcaccgatgg cgtggttgtg
    22801 cgagcggcga aagaacccga atcccgccat tggctaccag gccaggcaga atggctggtg
    22861 gcctggaaat atcaacctgt agctcgggtt gccgaagtga aggcgattca gtttgcggtg
    22921 ggtaagagcg gtaaaatatc ggtggttgcg tcactcgcac ctgtcatgct tgatgataaa
    22981 aaagtccagc gggtgaatat tggttccgtc aggcgctggc aggagtggga tattgcgcct
    23041 ggtgatcaga tcctcgtcag ccttgccggt cagggtattc ctcgcattga tgatgtggtg
    23101 tggcgcggtg cagaacgtac aaaaccgaca ccgccagaaa accgctttaa ctcgttgacc
    23161 tgctactttg cttctgatgt ttgtcaggaa cagttcattt cacgcttagt ctggctgggg
    23221 tcaaaacagg ttcttgggct ggatggcatt ggtgaggccg gctggcgctc gctgcatcag
    23281 actcatcgct ttgagcatat cttttcctgg cttttattaa cgccagagca attacagaac
    23341 acgccgggga tcgcgaaaag taaaagtgcg cagctatggc atcagtttaa tctggctcgt
    23401 aagcagcctt ttactcgctg ggtgatggca atgggaatac cgctaacccg ggcggcgctt
    23461 aatgccagtg atgaacggtc ctggtcgcaa cttttattga gcacggagca gttctggcag
    23521 cagctgccgg ggactggatc ggggcgtgtc agacaggtta ttgaatggaa ggaaaatgcg
    23581 caaatcaaga agctgggcag ttggctggct gcccagcaga tcacaggttt tgaaccttag
    23641 tgtccttcgt ggctgtagta aaagaccggt aatcccagct taaggcgtag cgccagtaag
    23701 cgggcaaaaa agccgaatac cagggtggag ataattacca catcatggtt agaaacatag
    23761 tgttgcagcg cgatgtacag cacggcggag gcaaatgaga caccggcata caactctttc
    23821 tggaatacca gcgggatgcg tttacagaac atatcgcgca aaacgccgcc aaatacgccg
    23881 gtcgtcacag ctgcgacaac ggcaatgatc gggccgtgtc ccatatccag tgcaacctgt
    23941 gcgccaatga tagaaaagac caccagtccg agcgcatcga gcaccagaaa cactttgcgc
    24001 aggtaaggca ttacaggagc aacgatagtg gtgagtactg cggcggtagc gacaataatc
    24061 acatattccg ggtgtttgac ccagccgagc ggatagtggc ccagcagaat atcgcgcact
    24121 gaccctccgc caattgccgt ggcggtcgca ataataatta cgccaaatgt atccatgcgc
    24181 cgtcgtccgg ccgccagcgc ccccgtcatg gcttcggcag taatgccaac caaatacaaa
    24241 atgtgtaaca gcatggttct ctccctaaaa atctgtctgg cagggtagcg atttgtgcgt
    24301 attgtcacga ttgagatttt ttaaggtgag aggaaattga taacttgtag ttatgtgaaa
    24361 tcgctatttt ctgtagcgta gatgcattct tctgacttct tgtctgagaa aatgcataag
    24421 aaaaaatagg ttaaaattga ggggtggaaa agaaaggtgc aatgtttagc cattgtgttc
    24481 accgcacctg gactttggct tacgcatcgg gtatctcaat caacaaaaag agtttttagt
    24541 ttctgctatt tcttctctgt aatttttaca ctgttttcca actgcttgat gttgacctgc
    24601 ggggtcggca actcctcagg catggttccg ccaagttcct gaatggtttg cctcaccttg
    24661 cgacccacgt caaagtgtgt ggtgttagct tgctgttttg aattcacgcc atcccgcttg
    24721 agtttttctt ctgtttgggt agctcgaaag agattagccg ccagttctgt tgaacccata
    24781 tgatccagga tcttctgact ctttttcagc cccttccgct gatggatggc tttctgatct
    24841 aatccaccat acagcccccg gtaaccatga ttctgaaata tggcgaaatc gatagctgtc
    24901 gctaccccag cttgctgcgc tgcctcaact aattgtttgt tatgttcttt caactcattt
    24961 ctcagaaaaa gacgcttttc gtcttcacga agttgcctga aagcttcatc gtcagccagc
    25021 tcctgccgtc gggtctggat agcaaaataa gtttgccctg ccgcaataac cggtttcgca
    25081 gggtcgccgt tttgtactac caggtaacag gcatagcggg agagtacgat gtcttttaat
    25141 gctcttttgg catttgagcc aattttgacc atatcgagga tctcctcgaa atggtcatca
    25201 attgtgtgac cactattcag acaagcttct ttggcgcgcg ttaatacagg tataaagtga
    25261 cgatactctg aataacccag tagttttcca agttgccttg ctgaccattg ttctgctccg
    25321 tttgcattaa tcagctttat ctcttcaaaa ggttgatgat gttcgttcat tgcccactcc
    25381 atgtgatatc gattgcaagg aaagtaagtc tacaactgtg tttatataca gttgtaagta
    25441 tctcacgttc tcactgcgct gggcaaaaaa acatcgcaaa atctggagca ggcacgcaaa
    25501 acagaattga atgttgcggg gaggaaaaat gccgctctgt ttaagtgggc ggcatttgtg
    25561 aaggctgaaa aggaagaatt ggcgttacga gttattcaat gttctggatc tgctcgcgca
    25621 tctgctcaat caacactttc agctcgatgg cagaattcgt cacttcggca ttgatagatt
    25681 tcgacgcaag agtgttcgac tcgcggttga actcctgcat cataaagtcc agacgacgac
    25741 caaccgcttc ttttttcttc agaatgttgt aggtctcttt gacatgcgct tcgaggcggt
    25801 ccagttcttc ggcaacgtca attcgttgtg ccagcagaac caactcctgc tccagacggt
    25861 tgttttccag ttgcacctga gcatcttcca gcttcgcgac cagacgctca cgctgccatt
    25921 gcaggatttc cggcatatgg gcgcggactt tgaccacttc ggcggtgacg ccttccagac
    25981 gctgctcgat caatgctttc agcgcctgac cttcggtttc gcgcgcgacg ataaagtcgt
    26041 ccagcgtacc atccaacgcc gcgagaattt cagcggcaat cgcgtcgaga tcctgctcct
    26101 gggctgccat cacgcccggc cagcgtagaa tatcaaccgg gttgatttcc ccttcgtcac
    26161 tctgcatttt tacccagttc gcggcagtta ccagctgttt agccagtttt tcgttgagga
    26221 tcagctcacc ttgcgcgcta acatctggct catagcgcag ggtacattcc actttaccgc
    26281 gcgtcaggcg agaacgaata cgctcacgaa cgacaggttc aaggctacgg aactgctccg
    26341 gcagacgaaa gtaagtttcc agataacgct ggtttaccga gcgcatttcc caggttgcgc
    26401 tcccccattc acccttgatt tcacgccggg cgtaggcggt catactgcgg atcatagacg
    26461 ttcctgttta taaaaggaga ggtggaagga ttatagccat cgatgccttg tcaggatagg
    26521 aataaccgcc ggaagtccgt ataatgcgca gccacatttg tttcaagccg gagatttcaa
    26581 tatgcgtcca gcaggccgta gcaataatca ggtgcgtccc gttaccctga ctcgtaacta
    26641 tacaaaacat gcagaaggct cggtgctggt cgaatttggc gataccaaag tattgtgtac
    26701 cgcctctatt gaagaaggcg tgccgcgctt cctgaaaggt cagggccagg gctggatcac
    26761 cgcagagtac ggcatgctgc cacgttctac ccacacccgt aacgcgcgtg aagcggcgaa
    26821 aggtaagcag ggtggacgca ccatggaaat ccagcgtctg atcgcccgtg ctcttcgtgc
    26881 ggcagtagat ttgaaagcgc tgggtgagtt caccattacg ctggactgcg acgtgcttca
    26941 ggctgatggt ggcactcgta ccgcgtcgat tacgggtgcc tgcgtggcgc tggcagatgc
    27001 gctacagaag ctggtggaaa acggaaagct gaaaaccaac ccgatgaaag ggatggtagc
    27061 cgcagtttct gtcggaattg tgaacggcga agcggtttgc gatctggaat acgttgaaga
    27121 ctcagccgca gagaccgaca tgaacgtagt gatgaccgaa gacgggcgca tcattgaagt
    27181 gcaggggacg gcagaaggcg aaccgttcac ccacgaagag ttactcacct tgttggcgct
    27241 ggcccgaggg ggaatcgaat ccattgtagc gacgcagaag gcggcgctgg caaactgatt
    27301 tttaaggcga ctgatgagtc gccttttttt tgtctgtaga aaagtaagat gaggagcgaa
    27361 ggcatgaaac catatcagcg ccagtttatt gaatttgcgc ttagcaagca ggtgttaaag
    27421 tttggcgagt ttacgctgaa atccgggcgc aaaagcccct atttcttcaa cgccgggctg
    27481 tttaataccg ggcgcgatct ggcactgtta ggccgttttt acgctgaagc attggtggat
    27541 tccggcattg agttcgatct gctgtttggc cctgcttaca aagggatccc gattgccacc
    27601 acaactgccg tggcgctggc ggagcatcat gacctggacc tgccgtactg ctttaaccgc
    27661 aaagaagcaa aagaccatgg tgaaggtggc aatctggtcg gtagcgcgtt acaaggacgc
    27721 gtaatgctgg tggatgatgt gatcaccgcc ggaacggcga ttcgcgagtc gatggaaatt
    27781 attcaggcca atggcgcgac gctggctggc gtattgattt cgcttgatcg tcaggaacgc
    27841 gggcgcggcg agatttcggc gattcaggaa gttgagcgtg attacaactg caaggtgatc
    27901 tctatcatca ccctgaaaga cctgattgct tacctggaag agaagccaga aatggcggaa
    27961 catctggcgg cggttaaggc ctatcgcgaa gagtttggcg tttaaagaaa ctcgccggat
    28021 gaaaagtcat ccggcgtcat attactgcaa ctgtgcggca attagcggcc agcgggcgtc
    28081 aaaatcatcc gtcgggcggt atttaaactc gctgcggaca aagcgtgaca gcataccttc
    28141 acagaaggcc agcaactggc ttgccagcag ggtttcatcg gtggtgtaac cttcaccctc
    28201 acgcattctc ttttcacgca atacctgacg caactgcgct tcaatacgct cgaacagctg
    28261 gttgatgcgc ccttgcagac gatcctgttc gaacattagc gcatgaccag tgaggatgcg
    28321 agtcaggcca ggattacgct caccaaaacc gagaagcagc aacacaatca gacgcaggcg
    28381 cgctgtggtg tccttctcat ctttcagaat cagattgatg cgagtaatca ggctatcttc
    28441 gataaactca atcaggctat cgaacatgcg ggtcttactg gggaagtggc gatacagtgc
    28501 cgcttcggaa acgccgacag aggcggccag ttttgccgtc gtgatacgtt ggcttccatc
    28561 gctggattcc agcatcagcg ccagagactg aagtatttcc tcgcgacggt tccttttcgc
    28621 agtttgtttt tctgccatgt tacaaaatac ccctgaaaat aagcacttgc caggcggcac
    28681 ccacgctatg accgcaaacg aaatgtttgc ggctatgtta tgacgttatt cggatgcgta
    28741 tgtgttactg acgaccagag tgaccaaagc cgccttcacc gcggtcggtg gcgtcgaaat
    28801 cttccaccag attaaattca gcctgtacta ccggaacaaa aatcatctgg gcgatgcgtt
    28861 cgccaggttg aatggtgaag ctgtcctgac cacggttcca cacggaaatc atcaactggc
    28921 cctgatagtc agaatcgatc aatcctacca ggttaccaag cacgataccg tgcttatgtc
    28981 ccaatccgga gcgcggtagc atcattgccg ccagtgaagg atcggcaata tgaatcgcca
    29041 gcccggtcgg aaccagcgta gtgtcacccg gagccagttc tacggcgtcg tcgagacagg
    29101 cacgcaggtc aagtccggca gagccagagg tggcataagt cgggagcgga aattccttcc
    29161 caacgcgcgg gtccagaatc ttaacgtcga tttttttcat cataacgggt cacgatctcg
    29221 tcgagtaata attggccaag gagctctttg cgctcaagcg gtaagacttt atctccgtcc
    29281 tgccagaaaa ggtgtaatgc gttgttgtcg ctgttaaatc cttgagttgg ctgggaaaca
    29341 tcgttcgcgc agatcagatc aaggttttta cggatacgtt tttgccgggc gtattcttcc
    29401 acattatttg tttcggcggc aaatccaacg acgtaggggc gatggtcttt tagtgcggca
    29461 acgcctgcga cgatatcggg gtttttaacc atttttattg ttaattcatc accctgcgtg
    29521 gcctgctttt tgattttttc tggggccacg gtagctgcgc gataatccgc cacggcggcg
    29581 caaccgataa aaatattttg ctgctgtaca gatgcattca cggcggcttc catttccagc
    29641 gcggtcatca catcaacacg tttaacaaac ggtggcgtcg gtagtgaaac cggacctgat
    29701 accagcgtga cgttcgcgcc acgacgggca gcggcggcgg cgatagcaaa acccatcttg
    29761 cctgagctgt gattagagat ataacgcacc ggatcgagtg gttcacgcgt cgggccggcg
    29821 gtaatcataa tgttcagatg tttcaggtcg ttgacgggcg aaaaatgcgc taccgccata
    29881 tcaacaatgg ttaacggatc gagcattcgc ccagggccga tatcaccaca agcctgactg
    29941 ccactgtctg gcccccaaat gagcaaacca cgggaagcaa gcacctctaa attatgctgc
    30001 gtggcagcgg cacggtacat ctgctggttc atggcgggga gcacggctac aggcgcaggt
    30061 gtggccagac aaatcgtcga taccagatca ttcgccattc cggcagcaac acgggcaatc
    30121 aaatctgccg tggcaggggc gagaatcact aaatcagccc atttacccag ctcaatatgg
    30181 cccatagcgg cttcggctgc tgggtccagc agactgtcgg aaacgggata accagaaacc
    30241 gcctgcaagc taagtggggt gataaaggct tttgccgctt cggtcatggc tacgcggacg
    30301 tcggccccgc gctcgcgcaa acgacgcacc agttcagggg ttttataggc agcaataccg
    30361 ccgctgacgc cgagaacgat ttttttaccg gccaggctca tgatgatttt tcctgttggg
    30421 tgacaccaga agttggcgat tttatcacaa tacttttgtt gtcgtgcctt cacctgagaa
    30481 tcactttgcg aggcgctttc caggattgaa aactggccgt cgatttaacg gaacggctat
    30541 gacaggatgc gagcaccaca aaggaggtga aggtgaaaaa caattcacag ctgttgatgc
    30601 cgcgcgaaaa aatgctgaag tttggtatta gcgccttaac ggatgtcgag ctgctggcgc
    30661 tatttctgcg taccggaacg cgcggtaaag atgtattaac cctggcaaaa gagatgctgg
    30721 agaatttcgg ctctctttat ggcttgttaa cctctgaata tgaacaattt agtggtgttc
    30781 atggaattgg cgtggcgaaa tttgcccagt taaaggggat tgccgaactg gcgcggcgtt
    30841 actacaacgt gcggatgcgt gaagaaagcc ctttactcag cccggagatg acgcgggaat
    30901 ttttacaaag ccagctcacg ggtgaggagc gggagatctt tatggtgatc tttctcgact
    30961 cacaacacag ggttataacg catagccgtc ttttttccgg cacgctaaac catgttgaag
    31021 tccatcctcg ggaaattatc cgcgaagcga taaaaataaa tgcctcggcg ctgatccttg
    31081 cacataatca cccttcgggt tgtgctgaac ccagtaaagc ggataaactc attactgaac
    31141 ggataataaa gagttgtcag ttcatggatt tacgcgtgct cgaccatatc gtgattgggc
    31201 gtggagagta tgtttctttt gccgaacgcg gctggattta acccgctatg cgcgatcctt
    31261 cgggatcttt gtctgttcgg gacttgagca catcgctgag tcagcgtata ctacgccacc
    31321 tttgagaatc tcgggtttgg catttgggcc tggcaatcga gagttcacat agaactgcga
    31381 tgaccgggct gtaaagcctg acgaggcgcc aataccccat acgaagctcg agctaatttg
    31441 atttttggag aatagacatg tcccgagtct gccaagttac tggcaagcgt ccggtgaccg
    31501 gtaacaaccg ttcccacgca ctgaacgcga ctaaacgccg tttcctgccg aacctgcact
    31561 ctcaccgttt ctgggttgag agcgagaagc gttttgtcac cctgcgcgta tctgctaaag
    31621 gtatgcgtgt aatcgataaa aaaggcatcg atacagttct ggctgaactg cgtgcccgtg
    31681 gcgaaaagta ctaagtactt agaggaaata aatcatggct aaaggtattc gtgagaaaat
    31741 caagctggtt tcttctgctg gtactggtca cttctatacc actacgaaga acaaacgtac
    31801 taagccggaa aaactggaac tgaaaaaatt cgatccagtt gttcgccagc acgtgatcta
    31861 caaagaagcg aaaatcaaat aatttttgct ttgatgtaac gaaaaacctc gccccggcgg
    31921 ggttttttgt tatctgcctg cccccatatt gactgcatct gttcattcct ggagatgcta
    31981 tgcctgaatt acccgaagtt gaaaccagcc gtcgcggcat agaaccgcat ctcgttggtg
    32041 caaccattct tcatgcggtg gtgcgcaacg gacgcttgcg ctggccggtt tcagaagaga
    32101 tctaccgttt aagcgaccaa ccagtgctta gcgtgcagcg gcgggctaaa tatctgctgc
    32161 tggagctgcc tgagggctgg attatcattc atttggggat gtctggcagc ctgcgcatcc
    32221 ttccagaaga acttccccct gaaaagcatg accatgttga tttggtgatg agcaacggta
    32281 aagtgctgcg ctacaccgat ccgcgccgct ttggtgcctg gctgtggacc aaagagctgg
    32341 aagggcacaa cgtgctggcc catcttggac cggagccgct tagcgacgat ttcaacggtg
    32401 agtacctgca tcagaagtgc gagaagaaaa aaacggcgat taaaccatgg ctgatggata
    32461 acaagctggt ggtaggcgta gggaatatct atgccagcga atctctgttt gcggcgggga
    32521 tccatccgga tcggctggcg tcatcactgt cgctggcaga gtgtgaattg ttagctcggg
    32581 tgattaaagc ggtgttgctg cgttcgattg agcagggcgg tacaacgctg aaagattttc
    32641 tgcaaagtga tggtaaaccg ggctatttcg ctcaggaatt gcaggtttac gggcgaaaag
    32701 gtgagtcgtg tcgggtgtgt ggtacgccga ttgtggcgac taaacatgcg cagcgggcaa
    32761 cgttttattg tcggcagtgc cagaagtaat tgatgcgcgc cggatggcat accatccggc
    32821 ataaactcta cgctaacttc gccatcagcg cctgatggac attctccggc aggaaatggg
    32881 tgacatcgcc ctgatggcgc gccacctctt tcaccaacga tgaagagata aacgaccact
    32941 ctttcgacgg catcagaaac acactttcca gttccggcat taagtggcga ttcatatgcg
    33001 ccagctgcat ttcatattca aaatccgcca ccgcacgcag gccacgaatc agcaccgtag
    33061 cgtgttgatt acgggcgaag ttcgccatca aatcactaaa cccgaccact tccacgttcc
    33121 ccagatgcgc ggttgcctgc tgtgccagcg ccacacgctc ttccagagta aacatcggtt
    33181 ttttactggg actggcggcg atcgccagaa taacgtgatc gaacatttgc gtggcgcgcg
    33241 tcacgatatc gatatgacca ttggtaatgg gatcgaaagt acccggataa atcgcccgtt
    33301 tttgcataac aacctcaatg cgttttcggt ggcaggtaag gttccagcag ttgaagcaga
    33361 cgctgtagcg cgccctggtt ttgatacagt acttcaacgg catgacggcc atagaaacta
    33421 cggtaatcgg cgtcggtgag taaagaggaa acctcttttg caagcgacgt ggcatcggta
    33481 acggtaatca gcccgcttgc ctgctccagc cgcgcgcaaa tgtctttaaa gttaaaagta
    33541 tgaggcccca tcaataccgg aatagcgtgt gcggcagctt ccagcggatt atgcccacca
    33601 cgttcaacca gtgaaccgcc aacaaaggcg agatcggcaa tgccatacag taacatcaac
    33661 tcgcccatcg tatcgccaac cacaacctgc gtgctggtgg aggggacttc ccctgaagag
    33721 cgtgtgatat agcttagtcc agcctggcgg acaaggttaa tcgcatccgg gaagcgttcc
    33781 ggatgacggg ggaccaggat gagcaataaa ttcgggaatt gctgtaacaa tgcctgatgt
    33841 gcggcgatca ccacactctc ttcgccttcg tgagtgctgg tggcaatcca taccgggcgg
    33901 tgtggtgccc actggcggcg cagcgtcacg gctttagcag ccaactgcgg cgttacagaa
    33961 atatcgaatt tcaggctacc ggtaacggtt acctggttat tttttgcgcc cagcgccaca
    34021 aaacgtgcac catcttcttc attttgcgca gcaatcagcg taatacgacg cagcaagcga
    34081 cggacgaatt tacccagttt ggcataacct gcggccgagc gggcagagag tcgcgcgtta
    34141 gcgatcacca gcggaatttt acgtttatgt agcgccgcaa tcaggttagg ccatagttcg
    34201 gtttccataa tcaacactag tttagggtcg actttattca ggaaacgatt aagtgcatcg
    34261 ggcaggtcat atggcagata aacgtgctga acatccttcc cgaaagccga ttgtacgcgc
    34321 tccgaaccgg ttggcgtcat ggttgtaacg gtaatcggta aatcaggata acgatggcgc
    34381 agcgcgcgca ccaacgggat cgccgccaga gtttcaccga cggagacgga gtgcagcatg
    34441 atgccgcctg gttttagcgg atggcggtaa aaaccgtaac gttcacccca gcgttttcga
    34501 taggccggag ccttacgtcc gcgcacccag agccgtatcc agatcagcgg ctgaataagg
    34561 tagagaaggg cggtgtaaag caattcgagc atagtaaata gctgacttat ggatgtgctg
    34621 gggattctat gtatttagct gtggctttac cattactttt cccgtttttg acttaaatag
    34681 caatttaccc gccaattctt gctgaacatc tatacttttt ttgtattttt atgcgattca
    34741 ttgatttctg cagtacgtgc aggaaggaat cgcagagcaa cagtgaaaga atatggttgc
    34801 taatgatttt atttgattga tttctttatt gaaaatcaac aatgtgctta aaaatcattc
    34861 gggtcggatt gccgcgaaag aagtgataca ctagcgcgtc aatatcagtg cggtatcagt
    34921 attcaggtag ctgttgagcc tggggcggta gcgtgctttt tctgcttcac cttaatcgga
    34981 taatctcaac aaaaagagtt acttgtggat aagccatttc gaagaatttt gctcattaag
    35041 atgcgttttc atggggatat gttattaact actcccgtca ttagttcgct gaaaaaaaat
    35101 taccctgacg caaaaatcga tgtgctgctt tatcaggaca ccatcccgat cctgtctgaa
    35161 aatccagaga ttaacgcgct ctacggcata aaaaataaaa aagcaaaagc ctcagaaaaa
    35221 attgccaact ttttccatct catcaaggta ttacgtgcca ataagtatga ccttatcgtt
    35281 aatcttaccg atcaatggat ggttgctata ctggtgcgct tattaaatgc ccgtgtgaaa
    35341 atttcccagg attatcatca tcggcagtct gctttttggc gtaaaagttt cacccatttg
    35401 gtgccgttgc agggtggaaa tgtggtggaa agtaacttat ccgtgctgac accactggga
    35461 gttgattcgt tggtgaagca gacaaccatg agttacccgc ctgcaagctg gaaacgtatg
    35521 cgtcgcgaac ttgatcacgc tggtgttgga caaaattatg tggttatcca acctacggcg
    35581 cggcaaatct tcaaatgctg ggacaacgcc aagttttccg ctgtgattga tgccttacat
    35641 gctcgtggtt atgaagtcgt tctgacgtcc ggcccagata aagacgatct ggcctgcgtc
    35701 aatgaaattg cgcagggatg ccagacgcca ccagtaacgg cgctggccgg aaaggtgacc
    35761 ttcccggaac ttggtgcgtt aatcgatcat gcgcagctgt ttattggcgt tgactccgca
    35821 ccggcgcata ttgccgctgc agttaatacg ccgctgatat cgctgtttgg tgcgacagac
    35881 catattttct ggcgtccctg gtcaaataac atgattcaat tctgggcggg agattaccgg
    35941 gaaatgccaa cgcgcgatca gcgtgaccga aatgagatgt atctttcggt tattccggcg
    36001 gcagatgtca ttgctgctgt cgataaatta ctgccctcct ccacgacagg tacgtcgtta
    36061 tgatcgttgc tttttgttta tataaatatt ttccctttgg cggtttgcag cgcgatttta
    36121 tgcgtattgc tcagacagtc gccgcccgag gtcatcatgt tcgggtttat acccagtcgt
    36181 gggaaggcga atgccctgat gtatttgaac tgatcaaagt gccggttaaa tcgcatacca
    36241 atcacgggcg caatgcggag tattttgcct gggtgcaaaa acatttacgc gaacatcccg
    36301 tcgataaagt cgttggattc aacaaaatgc cggggctgga cgtttattat gccgctgatg
    36361 tttgttatgc cgagaaagta gcgcaggaaa aaggcttttt ctatcgcctg acgtcacgtt
    36421 atcgccatta tgccgccttt gagcgggcaa ccttcgaaca gggcaagccg acacagctgc
    36481 tgatgctgac agataagcaa atcgccgatt tccagaaaca ttatcagact gaagcggagc
    36541 gttttcatat tctgccaccg gggatttatc ctgatcgtaa atatagccag cagccagcta
    36601 atagccgtga aatcttccgt aagaagaatg gaataaccga acaacaatat ttattgttgc
    36661 aggtcggttc agacttcacg cgtaaaggtg tcgatcgttc cattgaagca cttgcttcgt
    36721 taccggattc gctgcgccac aacacattgc tatatgttgt tgggcaggat aaaccgcgaa
    36781 aatttgaggc gctggcagaa aaacgcggcg tgcgcagtaa tgttcacttc ttctcggggc
    36841 gcaacgatgt ctcggaatta atggcggcgg cggatttatt actgcatcct gcctaccagg
    36901 aagcggcggg aattgtgctg ctggaagcga ttactgcagg attaccggta ctaacaactg
    36961 ccgtttgtgg ctatgcgcat tatattgtcg acgccaattg cggcgaggct attgctgagc
    37021 cattccgcca ggaaacattg aatgagattt tacgcaaagc gttaacgcaa ttttcattgc
    37081 gccaggcctg ggcggaaaat gcgcgacatt atgctgatac acaagattta tacagtctgc
    37141 cagagaaagc ggcggatatc ataacgggtg gtctggatgg ttgaacttaa agagccgttt
    37201 gccacgttat ggcgcggaaa agatcctttt gaggaagtta aaactttgca gggtgaggta
    37261 tttcgtgaac tggaaactcg ccgtactctg cgctttgaaa tggcgggcaa aagctatttt
    37321 ctcaaatggc atcgcggcac gaccctgaaa gagataatca aaaatttact ctcattgcgg
    37381 atgccagtat taggcgcaga ccgcgaatgg aatgcgattc atcgactgcg ggatgtcggc
    37441 gttgatacta tgtatggggt ggcattcggc gaaaaaggca ttaatccgct caccagaacc
    37501 tcgtttatta taaccgaaga tctgacacca accatcagtc tggaagatta ctgtgctgac
    37561 tgggcgacta accctccaga tgttcgcgta aagcgtatgc ttattaagcg tgtcgcgacg
    37621 atggtgcgcg atatgcatgc tgcgggcatt aaccaccgtg actgttatat ctgtcatttc
    37681 ctgctgcact tgcctttttc cggtaagaaa gaggagttaa aaatttcggt aattgatctg
    37741 caccgggcgc agcttcgcac gcgcgttcca cgtcgttggc gcgataaaga tcttataggg
    37801 ctttattttt cttcgatgaa tattggcctg actcagcggg atatctggcg gtttatgaaa
    37861 gtgtattttg tcgcctcgct taaagacatt ctcaagcagg aacaaggact gctgtcgcaa
    37921 gcagaagcaa aagccacaaa aatcagggaa agaacgattc gaaaatcgtt gtaatttccg
    37981 gagagaaata tgagtgccca ctattttaat ccacaagaga tgatcaataa gacaatcatc
    38041 ttcgatgaaa ggccagcggc gtcagtggca tcatcattcc atgttgctta tggcattgat
    38101 aaaaactttc tttttggttg tggtgtttca atcacgtcag ttttgttaca taacaacgac
    38161 gtaagttttg ttttccacgt ttttattgat gatatccctg aagccgatat ccagcgttta
    38221 gcccaattgg cgaaaagcta tcgtacctgt atccagatcc atctagtaaa ttgtgaacgg
    38281 cttaaggcat taccgacgac caaaaattgg tctattgcca tgtatttccg ttttgtaatt
    38341 gcagattact ttattgatca acaagataag atcctttacc tggatgctga tatcgcctgt
    38401 cagggaaact taaagccgct gataacaatg gatcttgcca ataacgttgc tgctgttgtt
    38461 actgaacgcg atgctaactg gtggtcgtta cggggtcaaa gtctgcagtg taatgaactt
    38521 gaaaagggtt actttaattc aggtgtcctg ttaattaata cactagcgtg ggcgcaggag
    38581 tccgtttctg ctaaagcgat gtcgatgctt gctgataaag ccatcgtttc ccgtttaacc
    38641 tatatggatc aagatatcct taatcttatc ctgttaggga aagttaaact cattgaagct
    38701 aaatacaata cgcaatttag tttaaattat gaattaaaaa aatcatttgt ttgtccaatt
    38761 aatgatgaaa ccgtattaat tcattatgtc ggcccgacaa aaccctggca ttactgggcc
    38821 ggttatccaa gtgcgcaacc ttttatcaaa gccaaagaag catcgccctg gaaaaatgaa
    38881 ccgttaatgc ggccagttaa ctcaaactat gctcgttatt gcgctaagca taattttaaa
    38941 caaaaca
//
LOCUS       DAZGQL010000043        38686 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.043, whole genome shotgun sequence.
ACCESSION   DAZGQL010000043 DAZGQL010000000
VERSION     DAZGQL010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38686
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.043"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            225..1880
                     /gene="aslA"
                     /locus_tag="ACHKIR_002449"
     CDS             225..1880
                     /gene="aslA"
                     /locus_tag="ACHKIR_002449"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase AslA"
                     /protein_id="HFO9990182.1"
                     /translation="MEFSFSPKLLVVAVAAALPLMANAADTPSTATARKGFAGYDHPN
                     QYLVKPATTIADNMMPVMQHPAQDKETQQKLAELEKKTGKKPNVVVFLLDDVGWMDVG
                     FNGGGVAVGNPTPDIDAVASQGLILTSAYSQPSSSPTRATILTGQYSIHHGILMPPMY
                     GQPGGLQGLTTLPQLLHDQGYVTQAIGKWHMGENKESQPQNVGFDDFRGFNSVSDMYT
                     EWRDVHVNPEVALSPDRSEYIQKLPFSKDDVHAVRGGEQEAIADITPKYMEDLDQRWM
                     EYGVKFLDKMAKSDKPFFLYYGTRGCHFDNYPNAKYAGSSPARTSYGDCMVEMNDIFA
                     NLYKALEKNGQLDNTLIVFTSDNGPEAEVPPHGRTPFRGAKGSTWEGGVRVPTFVYWK
                     GMIQPRKSDGIVDLADLFPTALDLAGHPGAKVANLVPKTTFIDGVDQTSFFLGTNGQS
                     NRKAEHYFLNGKLSAVRMDEFKYHVLIQQPYAYTQSGYQGGFTGTVMQTAGSSVFNLY
                     TDPQESDSIGVRHIPMGVPLQTEMHAYMEILKKYPPRAQIKSD"
     gene            complement(2038..3273)
                     /gene="aslB"
                     /locus_tag="ACHKIR_002450"
     CDS             complement(2038..3273)
                     /gene="aslB"
                     /locus_tag="ACHKIR_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFO9990183.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASETQNEVAFTWQGGEPTLLGLDFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSALQPLQVYDFLCDAGVEFIQFIPVVERLADETAAHAGLKLH
                     APGDIQGELTEWSVCPQEFGEFLVAIFDHWIKRDVGKVFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMLQQTIAEMIDSPQQQAFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLMVVNK"
     gene            complement(3420..3496)
                     /locus_tag="ACHKIR_002451"
     tRNA            complement(3420..3496)
                     /locus_tag="ACHKIR_002451"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3460..3462),aa:Pro,seq:tgg)
     gene            complement(3539..3625)
                     /locus_tag="ACHKIR_002452"
     tRNA            complement(3539..3625)
                     /locus_tag="ACHKIR_002452"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3589..3591),aa:Leu,seq:cag)
     gene            complement(3646..3721)
                     /locus_tag="ACHKIR_002453"
     tRNA            complement(3646..3721)
                     /locus_tag="ACHKIR_002453"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3686..3688),aa:His,seq:gtg)
     gene            complement(3780..3856)
                     /locus_tag="ACHKIR_002454"
     tRNA            complement(3780..3856)
                     /locus_tag="ACHKIR_002454"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3820..3822),aa:Arg,seq:ccg)
     gene            complement(3959..5344)
                     /gene="yifK"
                     /locus_tag="ACHKIR_002455"
     CDS             complement(3959..5344)
                     /gene="yifK"
                     /locus_tag="ACHKIR_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO9990184.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPTAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLTVTAIYKVFGL
                     NRHGKAHKLEK"
     gene            complement(5535..6275)
                     /gene="wecG"
                     /locus_tag="ACHKIR_002456"
     CDS             complement(5535..6275)
                     /gene="wecG"
                     /locus_tag="ACHKIR_002456"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047241 - lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HFO9990185.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(6278..7630)
                     /gene="wzyE"
                     /locus_tag="ACHKIR_002457"
     CDS             complement(6278..7630)
                     /gene="wzyE"
                     /locus_tag="ACHKIR_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HFO9990186.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADAPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(7627..8706)
                     /gene="rffT"
                     /locus_tag="ACHKIR_002458"
                     /gene_synonym="wecF"
     CDS             complement(7627..8706)
                     /gene="rffT"
                     /locus_tag="ACHKIR_002458"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HFO9990187.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCLALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFYWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDSSLNTMVNDRQREVKMTILVGNSGDRSNEHIAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQVLSEKLEFDAYLTLLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(8703..9953)
                     /gene="wzxE"
                     /locus_tag="ACHKIR_002459"
     CDS             complement(8703..9953)
                     /gene="wzxE"
                     /locus_tag="ACHKIR_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HFO9990188.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQELVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(9955..11085)
                     /gene="rffA"
                     /locus_tag="ACHKIR_002460"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(9955..11085)
                     /gene="rffA"
                     /locus_tag="ACHKIR_002460"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HFO9990189.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKYNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(11090..11764)
                     /gene="rffC"
                     /locus_tag="ACHKIR_002461"
                     /gene_synonym="wecD"
     CDS             complement(11090..11764)
                     /gene="rffC"
                     /locus_tag="ACHKIR_002461"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HFO9990190.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRISPEAPLLTPDALAPWSRV
                     QAKIAASNTDELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRSASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(11742..12623)
                     /gene="rfbA"
                     /locus_tag="ACHKIR_002462"
     CDS             complement(11742..12623)
                     /gene="rfbA"
                     /locus_tag="ACHKIR_002462"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFO9990191.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTQGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(12642..13709)
                     /gene="rffG"
                     /locus_tag="ACHKIR_002463"
     CDS             complement(12642..13709)
                     /gene="rffG"
                     /locus_tag="ACHKIR_002463"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFO9990192.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFVETNI
                     VGTYTLLEAARAYWNALTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SVSKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVAHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(13709..14971)
                     /gene="wecC"
                     /locus_tag="ACHKIR_002464"
     CDS             complement(13709..14971)
                     /gene="wecC"
                     /locus_tag="ACHKIR_002464"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0089714 - UDP-N-acetyl-D-mannosamine
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HFO9990193.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGELADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(14968..16098)
                     /gene="wecB"
                     /locus_tag="ACHKIR_002465"
     CDS             complement(14968..16098)
                     /gene="wecB"
                     /locus_tag="ACHKIR_002465"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HFO9990194.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPNKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(16154..17200)
                     /gene="wzzE"
                     /locus_tag="ACHKIR_002466"
     CDS             complement(16154..17200)
                     /gene="wzzE"
                     /locus_tag="ACHKIR_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HFO9990195.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIVFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYFRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(17212..18315)
                     /gene="wecA"
                     /locus_tag="ACHKIR_002467"
     CDS             complement(17212..18315)
                     /gene="wecA"
                     /locus_tag="ACHKIR_002467"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0036380 -
                     UDP-N-acetylglucosamine-undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HFO9990196.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(18555..19814)
                     /gene="rho"
                     /locus_tag="ACHKIR_002468"
     CDS             complement(18555..19814)
                     /gene="rho"
                     /locus_tag="ACHKIR_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFO9990197.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(19899..20000)
                     /gene="rhoL"
                     /locus_tag="ACHKIR_002469"
     CDS             complement(19899..20000)
                     /gene="rhoL"
                     /locus_tag="ACHKIR_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HFO9990198.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(20141..20470)
                     /gene="trxA"
                     /locus_tag="ACHKIR_002470"
     CDS             complement(20141..20470)
                     /gene="trxA"
                     /locus_tag="ACHKIR_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HFO9990199.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            20601..21866
                     /gene="rhlB"
                     /locus_tag="ACHKIR_002471"
     CDS             20601..21866
                     /gene="rhlB"
                     /locus_tag="ACHKIR_002471"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HFO9990200.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAQATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            22000..23484
                     /gene="gppA"
                     /locus_tag="ACHKIR_002472"
     CDS             22000..23484
                     /gene="gppA"
                     /locus_tag="ACHKIR_002472"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008894 -
                     guanosine-5'-triphosphate,3'-diphosphate diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0015974 - guanosine pentaphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HFO9990201.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLAQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLTVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFYDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIDQESQWQSYVHWPLEVH"
     gene            complement(23531..25552)
                     /gene="rep"
                     /locus_tag="ACHKIR_002473"
     CDS             complement(23531..25552)
                     /gene="rep"
                     /locus_tag="ACHKIR_002473"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HFO9990202.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPAQAAAEAKGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            25639..25920
                     /gene="ppiC"
                     /locus_tag="ACHKIR_002474"
     CDS             25639..25920
                     /gene="ppiC"
                     /locus_tag="ACHKIR_002474"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HFO9990203.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(25966..27441)
                     /gene="ilvC"
                     /locus_tag="ACHKIR_002475"
     CDS             complement(25966..27441)
                     /gene="ilvC"
                     /locus_tag="ACHKIR_002475"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HFO9990204.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETVTEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            27591..28481
                     /gene="ilvY"
                     /locus_tag="ACHKIR_002476"
     CDS             27591..28481
                     /gene="ilvY"
                     /locus_tag="ACHKIR_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HFO9990205.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLSTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLDNSPEPVRNRVMILERSDEKTPFELGVCV
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(28478..30022)
                     /gene="ilvA"
                     /locus_tag="ACHKIR_002477"
     CDS             complement(28478..30022)
                     /gene="ilvA"
                     /locus_tag="ACHKIR_002477"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HFO9990206.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(30025..31875)
                     /gene="ilvD"
                     /locus_tag="ACHKIR_002478"
     CDS             complement(30025..31875)
                     /gene="ilvD"
                     /locus_tag="ACHKIR_002478"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HFO9990207.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDDSALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDTLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(31940..32869)
                     /gene="ilvE"
                     /locus_tag="ACHKIR_002479"
     CDS             complement(31940..32869)
                     /gene="ilvE"
                     /locus_tag="ACHKIR_002479"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HFO9990208.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(32889..33152)
                     /gene="ilvM"
                     /locus_tag="ACHKIR_002480"
     CDS             complement(32889..33152)
                     /gene="ilvM"
                     /locus_tag="ACHKIR_002480"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HFO9990209.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(33149..34795)
                     /gene="ilvG"
                     /locus_tag="ACHKIR_002481"
     CDS             complement(33149..34795)
                     /gene="ilvG"
                     /locus_tag="ACHKIR_002481"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HFO9990210.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDILGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVSALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNIDDWQQHC
                     AQLRDEHAWRYDHPGDAIYAPLLLKQLSDRKSADCIVTTDVGQHQMWAAQHIVHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITHKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(34798..34848)
                     /gene="ilvX"
                     /locus_tag="ACHKIR_002482"
     CDS             complement(34798..34848)
                     /gene="ilvX"
                     /locus_tag="ACHKIR_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide IlvX"
                     /protein_id="HFO9990211.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(34935..35033)
                     /gene="ilvL"
                     /locus_tag="ACHKIR_002483"
     CDS             complement(34935..35033)
                     /gene="ilvL"
                     /locus_tag="ACHKIR_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HFO9990212.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            35386..36906
                     /gene="yifB"
                     /locus_tag="ACHKIR_002484"
     CDS             35386..36906
                     /gene="yifB"
                     /locus_tag="ACHKIR_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HFO9990213.1"
                     /translation="MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKHALERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVQGENSTTVKQRVMAARERQFKRQNKLNAWLDNPEIRQFCKLESEDAQWLEET
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(36932..37270)
                     /gene="maoP"
                     /locus_tag="ACHKIR_002485"
     CDS             complement(36932..37270)
                     /gene="maoP"
                     /locus_tag="ACHKIR_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HFO9990214.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            37389..38228
                     /gene="hdfR"
                     /locus_tag="ACHKIR_002486"
     CDS             37389..38228
                     /gene="hdfR"
                     /locus_tag="ACHKIR_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HFO9990215.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDVHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IATLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(38323..38398)
                     /locus_tag="ACHKIR_002487"
     tRNA            complement(38323..38398)
                     /locus_tag="ACHKIR_002487"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(38363..38365),aa:Trp,seq:cca)
     gene            complement(38407..38483)
                     /locus_tag="ACHKIR_002488"
     tRNA            complement(38407..38483)
                     /locus_tag="ACHKIR_002488"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(38447..38449),aa:Asp,seq:gtc)
     gene            complement(38538..38653)
                     /gene="rrf"
                     /locus_tag="ACHKIR_002489"
     rRNA            complement(38538..38653)
                     /gene="rrf"
                     /locus_tag="ACHKIR_002489"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         9469 a        10570 c         9756 g         8891 t
ORIGIN      
        1 cagcatcaat aatcaacgcg atataacatc accgccttac atatcacctg cgccagaggt
       61 aggattgaaa acgctctcct gattttccaa ttcattttct agataaataa ataatttatt
      121 tttatcacta ttatttatgc aatcatcctg tcagggagag ggatctcaat tatcaatgct
      181 taattacgtc atcattttga cgacatgaaa ccggagcatt ttgcatggaa ttttcgtttt
      241 cacccaaact tcttgttgtg gctgtcgccg ccgctcttcc actcatggcc aacgcagcag
      301 ataccccgtc aaccgccacc gcacgcaaag gctttgccgg atacgatcac ccaaaccagt
      361 atctggttaa accggcgacc actattgccg acaacatgat gccggtgatg cagcatcctg
      421 cacaagacaa agaaacccag cagaagctgg cagaacttga gaaaaaaacc ggtaagaaac
      481 cgaacgtggt tgttttcttg ctggatgatg tgggctggat ggatgttggc tttaacggcg
      541 gcggcgtggc ggtgggtaac cctacaccag atatcgacgc cgttgccagc caggggctga
      601 ttttaacttc ggcgtattct caaccgagtt cttccccaac ccgcgccacg attcttaccg
      661 gacaatactc catccaccac ggcattctga tgccgccaat gtacgggcaa ccgggcggtc
      721 tgcaagggtt aaccacgctg ccgcagttgc tgcacgatca gggctacgtc actcaggcca
      781 tcgggaaatg gcatatgggg gaaaacaaag agtcgcagcc gcagaacgtt ggctttgatg
      841 atttccgcgg ctttaactcg gtatccgata tgtataccga atggcgtgat gttcacgtca
      901 acccggaagt cgccctgagc ccggatcgtt ctgaatacat ccaaaaactg ccgttcagca
      961 aagatgacgt tcatgccgtg cgcggcggtg aacaggaagc cattgccgac attacgccga
     1021 aatatatgga agacctggat caacgctgga tggaatatgg cgttaagttc ctcgacaaga
     1081 tggcgaagag cgataagcct ttcttcctct actacggcac tcgcggctgt cacttcgata
     1141 actacccaaa tgcgaaatat gcgggtagct ctccggcgcg cacctcttac ggcgactgca
     1201 tggtggagat gaacgatatc ttcgccaatc tgtataaagc actggagaaa aacggtcagc
     1261 ttgataacac gctgattgtg ttcacctctg ataacggtcc ggaagccgaa gttccgccgc
     1321 acggtcgcac accgttccgc ggtgcgaaag gttctacatg ggaaggcggc gttcgtgtgc
     1381 caaccttcgt ttactggaaa ggcatgatcc aaccgcgtaa atctgacggc attgtcgatc
     1441 tggcggatct cttccctacg gcgctggatc tggcggggca tcctggggcg aaagtagcga
     1501 atttagtacc gaaaaccacc tttatcgatg gtgtggacca gacatctttc ttcctgggaa
     1561 caaatggtca gtctaaccgt aaggccgagc actacttcct caacggtaaa ctttctgctg
     1621 tgcgtatgga tgagttcaag tatcacgtcc tgattcagca accttacgct tatacccaga
     1681 gcggatatca gggtggattc accggcacag taatgcaaac ggcgggatcg tcggtgttta
     1741 acctgtacac cgatccgcag gaaagcgact ctatcggcgt gcgccatatt ccgatgggtg
     1801 taccgctaca gaccgaaatg cacgcgtata tggagatcct gaaaaaatat ccaccacgcg
     1861 cgcagattaa atctgactaa gccggcgcta tcgctgagat ctgcctttgc cggatgcgat
     1921 gctgacgcat cttatccagc ctacagaacg ctgcaattta ttgaatttgc aatgattgta
     1981 ggccggataa ggcgttcacg ccgcatccgg caatcaaccg caggcggccg ccgatttcta
     2041 cttattcacc accatcaaat gcgcctgcat aatgtcgctg gccggacgac cgtgcgccag
     2101 caaatcagcc attgctttaa gatatggcgg tagatggcgg aaataacgct gatacccggc
     2161 acacaaataa ttcagcccgg gtttgccgct ggcatcgagc ataaagcggt gtttcgggca
     2221 gcctccccag cacgctttta acacgttgca actgcgacac tgtgccggta actgcttgaa
     2281 tttatcttca ccaaatgcct gctgttgcgg ggaatcaatc atttctgcaa tcgtctgctg
     2341 aagcatattc cctagtcgat actgcggata aacatagtga tcgcaggcgt aaacatcgcc
     2401 gttgtgttca acaatcaccg agcgcccaca ggttggctga tgatggcaaa ccgcgcccgg
     2461 tgcgccgaca aaattggcga acgcccattc gatattcatt acgaaaacct tgccgacgtc
     2521 gcgcttaatc cagtggtcga aaatcgccac cagaaactca ccgaactctt gcgggcacac
     2581 cgaccattcc gtcagttcgc cctgaatatc gccgggagca tgtagcttaa gtccagcatg
     2641 agcagccgtt tcatcagcca ggcgctcgac caccggaata aactggatga actcaacacc
     2701 tgcatcacac agaaaatcat agacttgcaa aggttgtaac gcactggtgc gatttacgca
     2761 gaccagcacg ttatagtcga catgatgttt ttgcaggagc gtcagggcac gcatcaccag
     2821 cttatgcgtt ggtctgccgc ctttggtcac gcgatattga ttgtggatct cagccggacc
     2881 atccagcgat aacccaacaa gaaaatgatt ttctgccaga aatgcacacc attcgtcatc
     2941 aagcagcacg ccgttagtct ggaagctgtt acttatcttc ctgccagcac catatttcgc
     3001 ctgtagcgcc acggcacggc ggtaaaaatc cagcccgagt aacgttggtt cgccgccctg
     3061 ccaggtaaaa gccacttcgt tttgggtttc gctggcagcg atatagtgac ggacatacgc
     3121 ttccagcgtg tcatcgtcca tatgcgtgac tggcttttcg cggtaaaggg attgtttttc
     3181 gagataaaaa cagtagtcac agttcagatt acaatcggaa ccgctcggtt tcgccatcac
     3241 atgaaaagca cgcgttggaa cctgttgcag catggtcgct ccttgcctga agtcatcctt
     3301 caataagtta agggcgaaat cataccgtgg cgcaatgcgt tattacgggc gtttattgcg
     3361 aagggaaagt gacgaaatgc acagaaaaca aaaaaccccg ccgaagcggg gttcaaaatt
     3421 ggtcggcgag agaggattcg aacctccgac ccactggtcc caaaccagtt gcgctaccaa
     3481 gctgcgctac tcgccgaaat actgcttttt gaatttttag ttcaattctt taaagtcgtg
     3541 gtgcgatggg ggggacttga acccccacgt ccgtaaggac actaacacct gaagctagcg
     3601 cgtctaccaa ttccgccacc ttcgcattgt cacaacttct aataatgggg tggctaatgg
     3661 gattcgaacc cacgacaact ggaatcacaa tccagggctc taccaactga gctatagcca
     3721 ccactacaaa tcttgttacg cggtattact accaccgcag ctcaagcgcc gggactaaat
     3781 ggcgcgcccg acaggattcg aacctgagac ctctgcctcc ggagggcagc gctctatcca
     3841 gctgagctac gggcgcttag cgccgttgcg ggggtggata atacggactt cccacccctc
     3901 tgtctagtcc ctttttaaat aaattgcgcg tttggttacg gtttgtgcgc tttgctgctt
     3961 atttctccag tttatgcgct ttaccgtggc gattaagccc aaaaacttta taaatcgccg
     4021 tcaccgtcag cataaagatg atgccgacaa acagcgacat acgcgtatct tcattaaagt
     4081 acatgccgat caaaacgcaa atcaggaatg ccattgttac gtagttggcc cacgggaaca
     4141 ggattgagcg gaacggatgg ctggcaattg ccgctttatg cgcacgacga aaacgcagct
     4201 ggctaatcaa tatcacaaac catggcacca tccccggaag cacgctggca ctgtagacgt
     4261 agacaaacac acgctgcgga ttgggaatga tgtagttcag gcatgagcca attaacagga
     4321 tagcaataga tactgccaca cccgcaaccg gtacgccgtg acgggaaact ttcgccatcg
     4381 ccgtcggtaa ctgacggttt ttcgccaatg cgtagagcat acgtccgcaa ctgtacatgc
     4441 cgctgttaca gccagagagc gcagccgtca gcaccacaaa gttgataatg cccgccgctg
     4501 cggtaatgcc gattttggcg aaagtcagta cgaacgggct gccgttactg cctatttcat
     4561 tccacgggaa gatggtgacg ataacgaaaa tcgcgcctac gtagaaaatc aggatacgcc
     4621 acaacacttt gcctacggca ctgcgcagcg tcacctgcgg attcttcgct tcaccggcag
     4681 taatgccaat cagctccacg ccctggtagg acgccaccac aatacacaat gcggtcagga
     4741 accctttcca gccacccgca aagaaaccgc catgctctgt gagattgcta aaaccaatcg
     4801 actgcccgcc attgccaaaa ccaaagaaaa tcacgcccag gccaatgaca atcatcacga
     4861 taatcgtggt gactttgatc atcgcgaacc agaactcgat ttcgccgtac aaccgcactg
     4921 ccgccagatt cgccagcgcc accagcgcca ccgcgatcaa tgcgggtatc cactgcgcca
     4981 tctccgggaa ccagaactgg acataaacgc caatggcggt gatttctgag atccccaccg
     5041 ccatccacat aaaccagtaa gaccaggcgg tgagatagcc aaaaaacggg ctcatataac
     5101 gatgcgcata aacggcgaac gaaccggtaa ccggttcgag gaacaacatt tcgcccattg
     5161 aacgcatgat gaaaaagacg aacagcccgg cgatgatata ggccaacaat acggatggcc
     5221 cggcccattt cagggtactg gcggccccca taaacaggcc gacgccaatg gtgcccccca
     5281 gggcgatgag ttcgatatgt cgagcttcca gcccacgctg tagctctggt ttgttatctg
     5341 ccataaatcc tcgtgttgtg tttgcatgct ttccggtgtt accggttatc gttatgggta
     5401 catcgagtgt tgcgaatgtt ttcgtaattc aggagaaatg gcaaataaag cattaaaaat
     5461 ttgaatgctt tgtgtaataa aaaagcagac aggcgacgga gtgaccactc cgtcgcttta
     5521 caaagagagg aaaatcagag gttgccggtg tagtgccagc gtaaataacg cagcaaacga
     5581 agctgacgct taatgcggct cggctgcgaa agcaggcggt agagccactc cagccccagc
     5641 gtttgccaga ttttcggtgc gcgttttacg tgaccggtga aaacatcgta ggtcccgcca
     5701 acgcccatat acagcgcatc tggatgcacc agacggcagt cgcgcatgaa gatctcctgc
     5761 tttggcgatc ccatcgcaac ggtgacgatt tgcgctccgc tggcatgaat gcgttcaaac
     5821 agcgcctgac gctgctcggg tttaaaataa ccatcctgac tgccaacgat attcacattc
     5881 cactggttgc gcagtttagc ttcagtttgc gccagcactt caggtttacc gcccacaaga
     5941 aataccggcg tcccttcttt gcctgcgcgc gccatcagct cttcccagag atcggcaccg
     6001 gcaacgcggg aaacctgcgc ctgcgggtac tttttacgta ctgaacgtac aacgctgatg
     6061 ccatccgcat atttaaattc ggcagcgtta attagctccc tgacctcagc attatcttca
     6121 atggtcagca ttttttcggc attaatggca accagcgttc cctgcttaag ctgcccgtca
     6181 gcaaacagat aatcgagggc gtgctgcata tcacgccaac caatcaactg taagccacgc
     6241 agcgtatagg tcggtgccgt ggtgttgtta ttcattgtta tccttcaacc tgcgtccgga
     6301 gcgatgattt tgtacgttta tgaatgagtc cggcgctttc aaaaagccag tacaacagtt
     6361 ttgcgatcat cagacatgcg ccgaagacca cgataaaaaa gactacgcgt gagacaaacg
     6421 aatccagccc ttcacgtgcc agcacgatca tattgaagat cgccccgaag cagaaactgt
     6481 gcaatatcgc agccttatag cgattagtct cgcggttgcc cagctcatac agccagtcga
     6541 accatttgat gatcagacca accacaatcg ccccgagcgg gatgaacaac gcgccgccca
     6601 tcaccaccag tgagcctata agcgtaggcg agatcgccag cccggagtgg ttattcagca
     6661 cttcccaggt aaagtagttg gcggagttca gtaccatact cgggcgaccc ggccacagcc
     6721 aggaagggat aaagacatag aaatcgcgga caatgggagc caggccctgg aagtcgatgt
     6781 tgtcgtagtt ctgcaacagc aacgccagat tctcccacgg cgaaaaggta tcgcgggtga
     6841 gatagagaaa cgtatagaac gcttcatcgc cgctcacatt cattccataa cgttttagcg
     6901 ccagccagaa catgcccaca atccccagca cgcccgccgc cgccagcatc cacaacgaaa
     6961 tccagccgcg aataatgcca ataaacagga agatagcgaa tgcgatgatg atattggcgc
     7021 gagtgccgcc gacaatcatg taagtcaaca agccaaaggc aaccgtgctg acgaggaaaa
     7081 acagccacgc tttgctatcc tggcgcagaa aataaaccac cagcatcgcc gggatgaaaa
     7141 agtaaaagaa gcgttttaat gccacgccgg agacttcgct ggaaaagatc tgactgtaag
     7201 aattaagccg gaacagcaaa aagccgttgt gcatgaagaa gatgccgacg ctcaccagag
     7261 cgatgcccat caggatcacc cacgtgagat tggtctccac gcggttcatg gtaaacagcg
     7321 gacggcgcgg tgcatcagca acgcgtttgc gtagacgggt tttgtaggtg acatagtaaa
     7381 ccgcgtagaa gcagcctgca gaaagcaacg cctgcaacaa gatttctggc ggcgcgacac
     7441 caacatcaaa gcgaaatacc agcacgctgg tcagcggaaa gccgaagaaa aaggtgagca
     7501 aaaacagcaa tgaaaagaag acattgaagt taaagcgcac acggcgaaac tcaaaccagg
     7561 tcagcgtggc aataaacaac gtgcagagca gccagacaac aaacaggcca ctgaattgca
     7621 gcagactcat gcgacctccc cggcggcaat cgccaacgcc cgctgccagc cttgtagata
     7681 gttagggcta aagaaggcaa tggtgttttt atccaccgac gccaactggc gctgcgcttc
     7741 acgcacaata tcctcgttga gatcatcggt agtaaacagt actggcaaat gctgttccgt
     7801 catatcctgc cagaacggat tttcccggtt aagcacacaa ggaatgcccg cctgaatcag
     7861 taagcaaagc gtaccaatgc cctgctggcg ggcaaaaata aagtaaccga gatcgcactg
     7921 acgaagtagc gtcagatagg cgtcaaattc cagtttttcg ctcagaactt gcagattttc
     7981 ttcgctgaat aactccagtc ccgcctgacg aacttcctcg atgtacgctt cgttattagg
     8041 cggatatccc atcggcacca ccacttttac cgtatcgcca aattgctgat gaacggcgcg
     8101 caaggcagca atatgctcat tgctgcggtc accggagttc cccaccagaa tggtcatttt
     8161 tacttcacgt tgccgatcgt tcaccatcgt attaagagaa gagtccatcc gcgtcgggaa
     8221 gtacagcagt tcgccccgca cctttgggtg cgttttggca aaaaagctca aatcaccgcg
     8281 ggtggcaaat acacagccga ctcgcttttg cgccaggcga cgtagtgggt aaaaaagctt
     8341 atatctcaag ccactggaaa gttcgtacag gtctgccccc cagatatgcc agtaaaactg
     8401 gctgggctta atgccaccac tcagcagagc cagccacagt gtgggattga actgaccgtg
     8461 gaagaaaaaa cgctgctgac ggttagcttt tgctttcgcg atgaccgctt ccgccagcga
     8521 ttttttccca gggaaaaatt gcacagaaag cgccagacag ctatcactta agccgtcgtc
     8581 cttgccaaca accataaact cgcgcgcgtg ctcgctcgtc gcggccagcg cgtcgttgaa
     8641 aaaccgcaga acggttcggt tatggtgagg gatatccgat cccagtacgt gaatcagtac
     8701 agtcatgccc gcctacgcca gagtaaaaac acgccacaac aaagagaaaa atagacgata
     8761 taggttgcca tataggcctg cgctgcgccc agtgcaccat gcgcagggat tagccagtgg
     8821 gcaaatacca tcaataaagt gaactggctg acttccgcca gaatataaaa ccgcagtgac
     8881 gctttggcga tcaccagata accaaagaca taagcgccca cttttaacac atcacccact
     8941 aactgccagg caaagagatc gcgcatagcg gtaaatttat tcgacaacag cagccagata
     9001 gcaaaatcac gcagcaacca gacggtaaaa ctcgccgccg ccactgccgg taagacgaat
     9061 tttagcgatt taaccacttc ccgggtgata tcgcgctttt ccgttagccg cgacaacgtg
     9121 ggcagcaaat atacgctgaa cgatgccgta ataaattgca ggtaggcatc ggaaatactg
     9181 ctcacccctt gccagatccc cacctcatcc cagctatact gcgccgccag cagtttacgc
     9241 atcatgatgt aagcaacagg caaggtcacc gacgtaatca acgccatgag cgtaaatttg
     9301 ctcaactgcc ctgccagacc gttatcccag ctgggtttca gatagcttaa cgggatgaca
     9361 ccacgtttga tcaacatgat ggcggcagga attaccacca gcgcgggaat cagcgccaga
     9421 cccagcaacg ccccttcata accgcccaaa cggtaactga cgtagtacgc aagaacgcca
     9481 atcaagctgc caacaatcag cgataacgca ttacctgcgg catcgcgaaa gcctttcatc
     9541 aacgccagta acaggttgcc ccaggcgatc cccatttgca ccaacgccac taaacgcacc
     9601 agctcctgat agtcggtatt accaaacaat ccctggctga ttggcgcagc tgccagcaca
     9661 aaaaccagcg ccatcagcgt agagaaacca agtaccatcg ctgatgaagt gccgaccacg
     9721 cggcgcagct gttggggatt atcatggtac tgggcaacgt atttggttac gccgttaaag
     9781 atgcctgccc cggcaagcac gccgagcacg gtaatcaact ggcggaaatt tgccgccagc
     9841 ccaagccccg ccggaccaaa tgacactgcc agcaacttac cgaccagtaa cccggcacca
     9901 atcttgacca gtgtactggc cgcagtccac aaggacgctt ttgccaacga catatcagga
     9961 gaagtagttc aacaaggtcg caattaccgt acgctgattg acgggcgaca ggttgtagaa
    10021 caacggcagg cgcagcaggc gctcgctctc tttggtggtg tagcgatctt caccgtggaa
    10081 ctcaccaaag cgttcccccg cagggcaacc gtgcagcgga atgtaatgga acaccgccat
    10141 gatttccgct tctttcagaa agttaatcaa cgcgctccgg tcatcaatat cccgcagttt
    10201 aatgtagaac atatgcgcgt tctgcacgca gccatcggga atcgacggca gctcgatacg
    10261 cccggctttc gccagaggtg ctaacgcatc gtagtagttt tgccacagcg ccagacgttg
    10321 ctgattgata cgatccgctg cttccagttg cgcccacagg tacgcagctt gcaaatcgga
    10381 catcaaatag ctggagccga tatcgcgcca ggtatattta tcgacctgac cacggaagaa
    10441 ctggctgcgg ttagtgcctt tttcacggat gatctcggct cgttcgatca acgctttatc
    10501 gttaatcagc gtcgcaccgc cttcaccgcc cgccgtgtag tttttggttt catggaagct
    10561 aaagcagcca atatgaccaa tggttcccag tgcacgccct ttgtaagtgg acataacgcc
    10621 ctgagcggca tcttccacca caaacagatt atactttttc gccaacgcca taatggtgtc
    10681 catttcgcag gccacacccg cgtaatggac cggcacgata acgcgcgttt tgtcggtgat
    10741 cgccgcttca atcagcgttt cgtcgatgtt catagtgtcc gggcgaacat ccacaaagac
    10801 gattttcgcg ccacgcagca caaaggcatt ggcggtggag acaaaggtgt agctcggcat
    10861 gatcacttca tcgccaggct ggatatcgag cagcagcgcc gccatctcca gagaagcggt
    10921 gcaggacggc gtcaacagca ctttggcgct gccaaaacgt tgttccagcc actgctggca
    10981 gcgacgggta aaaccgccat cgccacacag tttgccgcta cccattgccg actgcatata
    11041 gtcgagttcg gttcccacca ccggcggtgc gttaaatgga atcatgtgat cacctgtata
    11101 accagtacgc ggtgctttct acattcgcac cactttgtat gtatcgttta agcgcggcgg
    11161 tgttgcccat ctgggtcgcc acccgcaaag ttgttttacc gcgagcatac gcccagttta
    11221 gcgctgtttg catcagctca gcacctgcac cgcgtccagc cagcaggcca attcgcgcat
    11281 ctgtcgcatt gagttcccgt aaagagacat agccgcgaat atcgccggac gccgaacgta
    11341 aaatgaggca ttgatgatca aaggtgccgc gcacggcatt ttcaatccac tgtgcataaa
    11401 agcgaccgct ggcgtcaggc gcataccacg gcgcacgaaa acggctttgc gcaaatgcgg
    11461 cgctggctaa ctgacgtaat gcgggaatat cggtctcttg tgccactaca gcaccactat
    11521 cactgacatt gtttacgggt agcgccaaat caacttcacc ttctaccagg gagaatccca
    11581 gctgttgcaa ggcatccagt tcatccgtat ttgatgccgc aattttggcc tgcacccgtg
    11641 accacggcgc taaggcgtct ggcgtcagga gcggtgcttc aggactaatg cgcacaatgg
    11701 cgctgttaac accaaagaag gcgttttccc aggttagtgg ctcaatactg gcgcggacgg
    11761 gcacgaagta actccagcag atattggccg tagccagttt tcgccagcga gctggcggcg
    11821 cgcttcacgc cttcgtcatc aagccagccg ttacgccagg cgatctcttc caggcaggca
    11881 atcttaaagc cctggcgttt ttccaccgtc tgcacaaagg tgctggcttc aatcaggctg
    11941 tcgtgagtgc cagtgtccag ccaggcaaat ccgcgcccga gcagttcaac ggtcaggttg
    12001 cccgcctcga ggtacatctg gttgatggag gtaatctcca gttcaccacg ctccgacggc
    12061 ttcacctgct ttgcgtactc cacgacttta ctgtcgtaga aataaagccc ggtcaccgcc
    12121 cagtttgact tcggctgttt tggcttttct tccagcgaga tagcgcggaa attgtcgtca
    12181 aactccacga cgccaaagcg ttccggatcc atcacctgat agccaaatac cgtcgcgcct
    12241 tgcgtgcgcg ccgcaacatg acgcagcttc ggactgaagc cctgtccgaa gaagatgtta
    12301 tcgcccagca ccagacaaga aggttcaccg ttgaggaagg tttcaccgat gataaaggcc
    12361 tgcgccagac cgtccggact gggctgttcg gcatattcca gctggatacc gaactcacta
    12421 ccatcgccca gcaggcgctg gaaataacct ttatcttccg gcgtagtgat gatgagaatt
    12481 tcgcggatac cggccagcat cagcaccgac agcggatagt aaatcattgg cttatcgtaa
    12541 atcggcaaca gttgcttcga tacgccgcgc gtaatcggat gcaatcgggt gccggaaccg
    12601 cccgccagga taataccttt catgtttgcc tccgctgaaa attagccttt cagacctaaa
    12661 cgctcgccct gatagctgcc gtcctgcacc tgcttccacc agctttcatt agccagatac
    12721 cactgaaccg ttttacgcat tccactttca aaggtttcct gcggcagcca gccaagttca
    12781 cgggcaattt tcgaagcatc aatggcataa cgcagatcat gccccggacg gtcagcgaca
    12841 aaggtgatca ggtcacgata gtgcgccacg ccgtgcggct tgttcggagc cagttcttcc
    12901 agcagctcgc aaatggtttc cacaacatcg agattcttac gctcgttgtg accaccaata
    12961 ttataggttt caccgacttt cccggtggtc gccacgcaat acagcgcgcg ggcgtgatct
    13021 tccacataca gccagtcacg gatttgctgc ccgttgccat ataccggcag cggtttaccc
    13081 gccagcgcgt tgaggatcat cagcgggatc agtttttccg gaaagtggta agggccgtag
    13141 ttattcgagc agttggtgat aagcgtcggc agaccatagg tccgcagcca ggcgcgcacc
    13201 agatggtcgc tgctggcttt tgacacggaa taagggctgc tcggcgcata cggcgtggtt
    13261 tcggtgaaga aatcatccgt cgagtgcagg tcaccatata cttcgtcggt agagatatga
    13321 tgaaaacgga acgctgattt tttatcttcc gtcagcgcat tccagtaagc ccgcgccgct
    13381 tcaagcaatg tataagtccc gacaatgttg gtttcaacaa acgctgccgg gccgtcaata
    13441 gaacggtcaa catggctttc ggctgccaga tgcatgacac agtctggctg atgctcagtg
    13501 aatacgcgcg ccagttctgc ccgatcgcag atatcaactt tctcaaaggc aaagcgctcg
    13561 ctttgcgcga ccggtgccag cgacatcaga tttccggcgt aggtcagctt atcgaccact
    13621 accaccgcgt cgctcgtttc gttgatgata taacgcacca gcgccgagcc aataaacccg
    13681 gcaccacctg ttatcagaat ttttctcatc agcgccagac tcctttggca tcgacgacat
    13741 actgctgatg gacattgtcg ccattgataa ctttgaactg actatgatcg accagcatca
    13801 ccagcacgtc tgccgttgcc agcgcctcgt caagctgcgc cagtgtacaa agcccggtca
    13861 gttttttcgg caactggtgg atgttaggct caacaaccag agtttcgccg ctatgccact
    13921 gggcgatcag ttcagcgatt tccatcgctg ggctttcgcg caggtcatca atattcggtt
    13981 taaacgccag accaaagcag gcgattttca gttcactggc gcgtttatcg gtagcagcaa
    14041 ggcagtcggc aaccgccgct ttcacctgat cgataaccca gaacggtttg tgatcgttca
    14101 cttcgcgcgc ggtacggata agccgcgcct gctgcgggtt ctgtgccacg ataaaccacg
    14161 gatcaacagc aatgcagtga ccgcccacgc caggaccagg ctgaagaata ttaacgcgag
    14221 ggtgacgatt cgccaggcga atcagttccc agacgttaat tccctgatcg gcacaaatca
    14281 gcgacagttc gttagcaaaa gcgatattca catcgcggaa gctgttttcg gtgagcttac
    14341 acatttccgc cgtccgcgag ttagtgacga cacactcacc ttcgaggaaa attttgtaca
    14401 gttcgctggc gcgggccgaa caaaccggcg tcataccacc aatcacgcga tcgtttttaa
    14461 tcagctcgac cataacctgg cccggtaaca cgcgttccgg gcagtaagca atgttgacgt
    14521 ccgccaactc gcccacctgc tgtgggaaag tgagatctgg acgcatctct gctaaccatt
    14581 ctgccatctt ctcggttgac cccaccggcg aggtggattc aaggatcacc agcgcgcctt
    14641 ttttcagcac tggcgcaatg gagcgagcag ccgattcaac gtaggtcata tctggctcat
    14701 gatcgccttt aaacggcgtg ggtacagcaa tcagccaggc atccgcttca actggcgtcg
    14761 tgctcgctcg taaaaaaccg ccttctacgg cagtttttac tacgctcgcc aaatcaggtt
    14821 cgacgatatg gatttcgcca cgattgatgg tatcaaccgc gtgttggttg atatcgacac
    14881 caatcacctg tttttgccgt gaggcaaacg cagctgctgt aggcagcccg atgtaaccca
    14941 gtccgataac agaaatggtc gcaaaactca tagtgatatc cgattatttt ttaacgcttc
    15001 cagaatgcga gagcatgcct gaccatcacc atacgggtta tgggcgcggc tcatagcttg
    15061 atattcgttt tcgtctttta aaagacgcgt cacttcctcg acaattcgct gcttatccgt
    15121 gcctaccaga cgcaccgtac ccgccgtcac cgcttccgga cgctcagtgg tatcgcgcat
    15181 taccagcacc ggtttcccca gcgaaggcgc ttcttcctga atgccgcctg agtcggtcaa
    15241 aatcagccag gcgtggttca tcagccagac aaacggtaaa tactcctggg gatcgatcag
    15301 aatgacattt ttcacgtgcc ccagaatgcg attgaccggc tctctgacgt tcgggttgag
    15361 atgcactgga tagacaatct ggatgtcctg gtgcgtggtg gcgatgtctg ccagcgcatg
    15421 gcagatttct tcaaagccac gaccgaagct ctcacgcctg tgaccggtca ccagaatcat
    15481 ttttttattg gggtcgataa acgggtaatt tgccgccagt tctgaacgca gcgtgtcgct
    15541 gctcatcacc tggtcacgca cccataacag tgcatcaatg actgtattac cagtaatgaa
    15601 gattcggcta tccgcaacgt tttcacgcag caagttttgc cgggaagttt cggttggaga
    15661 gaagtgatac atcgccagat gcccggtcaa tgtacggtta gcctcttccg gccacggcga
    15721 atagagatcg cccgtgcgca gaccagcctc aacgtgacca acaggaatac gctgataaaa
    15781 cgccgccagg ctggttgcca gcgtcgtcgt agtatcgccg tgaaccagca cgacgtctgg
    15841 tttgaactcg gcaagaatag gttttagccc ttccagaatc cgacaggtta tctctgtaag
    15901 gccctgtcct ggctgcatta tgttgagatc gtagtcaggt acaatggaaa agagtttcag
    15961 cacctgatcg agcatctccc gatgctgcgc agtgacgcaa actttagcct caaaaaaagg
    16021 atcttttgcc aacgcatgca ccaacggcgc catcttgatg gcttccgggc gcgtaccaaa
    16081 tacagtcagt actttcacat cgattctctt cgaataagcg gcgagcgcct ttgcgctcac
    16141 cgcagcagtg ttgctatttc gagcaacggc gggttaatgc gacaccagcc ccgatcagtc
    16201 ccccgacaat gccccacata atcatcagga aggcacgacg tgggctatcg cgttttaccg
    16261 gttcttccgg cgtacgaaaa tagcgatagg tctgaaaacg cggatccagg gttggaccaa
    16321 cattcagggt gtttaacatg gcccgattct gatcatagtc gagatcaaag gccggaccga
    16381 cggcctgtaa attttccagt cgagcctgga gcattggacg cccaagcagg aacatttctg
    16441 aatcaggtaa ttcctcggca ggcacatctg tcgcactgcg cgaaatatta tgctgctcag
    16501 caattttcag cgcctgttca atactgttca tccggcgatc gtagatggct ttcgccacct
    16561 cttcctgacg cttcacctga gctttcatct ggatggtgcg tgccgcccat gcgcctttca
    16621 gctcatcatt cagatggctg gctgcacgct ggctggcaaa agcaacatac tgacgtaaca
    16681 ggttattagc gtcaggcgca gtttcggcaa tcagcttcac gctgtcattg accgcgcggg
    16741 tgaagtcacc gggaataaac acgatgttgt tgatcatttc atccagcaac gccgcatcgg
    16801 ctttgctgtt gcccaccatc cgctgtttgt aatagtcggt ttgcagccag aactctctgc
    16861 gggtatccca cgaggccagt tgcataacaa actctttata agcttcgtcc atgaccgatg
    16921 gttggtcggc agaagccatg tttgaacgga catccaggtt acgcaaaaat tgctgctgtg
    16981 agtaatatcc ccccagcata ttcaccgttg gacgatcggt aatcgctgtc gagctccact
    17041 cctggcgagc aaaaaaagta tacgccagcg cgattaacgc aaacgccagc cccatgccaa
    17101 taatccacag cttcccagcc cacaaggtac gaaacaaccc acgaatatcc agttcatttt
    17161 cagcgtcttc ggccggtttc ccaggcattg gttgtgtcat cacatcctca tttatttggt
    17221 taaattgggg ctgccaccac gatttctacg cagtctgcgt tttacgcgct taataaagcg
    17281 agcaactttc caggcacgct tgatgcaata tccatagagg aagaatgcta gcaaaaagag
    17341 caccagcatg acccactccg ggacaaaatg agaatattct gccagcacgc caatggaagc
    17401 gagcagtgct gcggcaaggg taatcagcac aaacgcctgg cgggaagtaa acccggcacg
    17461 catgatcaaa tggtgaatat gctgacggtc tggagagaat gggctcatgc ctttacgcag
    17521 gcgacggtac ataatcgcca ccatatccat tagcggaatg gcgattatcc acaaagcggt
    17581 aaccgggctg atgggatgag ttttgccctg ggtcgtttcg agcaggatcc agataacggt
    17641 aaaaccaatc agcgtactgc ccgcatcccc cataaagact ttgtagcggc gacccaggat
    17701 accaaggtta agcatgatgt atggcaggat ggcggcgatc atcgcaaagc accagattgc
    17761 gagactggtt tgcccgtcga accacaaaat cataccgatt gctgcaaacg agacgcagga
    17821 caacccaccc agcaagccat caatgccatc aaccatgttg aacgcattaa tggccgccca
    17881 gacggcaaat agcgtcagga agtaaccaaa cggtccgagc accatctccc aggagccaaa
    17941 gatataacct aggctactga gataaagttt gccgaacacc atcataacaa tgccaacagc
    18001 ggcctgtatg gtggcacgga tttttacgct gatatcaaaa cggtcatcca gcgcgccaat
    18061 gaaaacaagc acaccagcac aagcgagata gagagatgca tgcggaatat agtaatcgac
    18121 aattccgaac gtgaagcaaa tccctgcgta aaccgaaatc cccccaacga gaggtatcaa
    18181 tccctggtga cgtttgcgga agttaggttt atccactaaa ccgacttttt ttgccacctt
    18241 acgggcaaaa aacagaaaca gtgtcgtgaa taaaaaaata ctgatgagat cagtactcac
    18301 tgtcagtaaa ttcacaatgc atgctctcag agaaaattat tagcagaagt ataaccacga
    18361 agacctttat tcagaaggga aagtccgttc cgaacccacc agtcctgtgc aaaaattaac
    18421 catttgatat gcaaattgca taacacacca gcaaaatcca ggaattttcc taacattgcc
    18481 agcgcggcat taagattaca gatataaaag caaaacgcca cgtaaacacg tggcgttttt
    18541 ggcataagac aaatttatga gcgtttcatc atttcgaaga aatcgtcatt ggtcttggtc
    18601 attgccagtt tattaatgag gaattccatt gcatcgattt cgcccatcgg gtgaatgatt
    18661 ttgcgcagga tccacatttt ctgcagttct tcctgagtag tgagcagctc ttctttacgg
    18721 gtaccggaac ggttgtagtc gattgccggg aagacgcgtt tttcagcgat cttacgagag
    18781 aggtgcagtt ccatgttgcc tgtaccttta aactcttcgt agataacttc gtccatcttg
    18841 gaaccggtat cgataagcgc ggtagcgatg atggtcaggc tgccgccctc ttccacgtta
    18901 cgcgccgcac cgaagaagcg tttcggacga tgcagggcgt tggcatccac accaccggtc
    18961 agcactttac ctgacgccgg aacaacggtg ttgtaagcgc gcgccagacg agtgatggag
    19021 tcgagcagga tgataacgtc tttcttgtgc tcaacaaggc gtttcgcctt ctcgatcacc
    19081 atttccgcga cctgaacgtg gcgagatgct ggttcgtcaa aggtagaagc aacaacttca
    19141 ccttttacca gacgctgcat ctcggttact tcttccggac gttcgtcgat cagcagaacc
    19201 atcagcacac aatccgggtg gttgtaagca atgctctgag caatgttctg cagcagcatg
    19261 gttttaccgg ctttcggcgg tgccacaatc agaccacgct gaccacgacc gataggtgat
    19321 gccagatcca gtacgcgagc ggttaaatct tcagtagaac cgttaccacg ttccatacgc
    19381 agacgagagt ttgcgtgcag cggggttaag ttctcaaaga ggattttgtt gcgggcgttt
    19441 tcaggtttgt cgaagttaac ttcgttaact ttcagcagcg caaaatagcg ttcaccttct
    19501 ttcggcgggc gaatcttacc agagatggta tcaccagtgc ggaggttgaa acggcggatt
    19561 tggctagggg aaacgtagat gtcatcagga ccggcgaggt aggagctgtc tgcggaacgg
    19621 aggaaaccaa atccatcctg caatatctcc agtacaccat caccaaagat atcttcgcca
    19681 ctctttgcgt gctgcttcag gatggcaaaa ataatgtcct gcttacgcat acgagccagg
    19741 ttttccagcc ccatattttc gccgagagtg atcagctcag aaaccggcgt attctttaat
    19801 tcggtaagat tcatagtggt gtgagttctt aaacttgggg taaatctcga acttaatgtt
    19861 gtgaatggta tggcaggatc atccatgcct gtttacggtc atcgactcat gtcttttcgc
    19921 tgcctggtca caggagagta cgcagaactg aaacgacaag acggattgag tgacgagcca
    19981 gaaatctgtt cacttcgcat ttaagataaa acgggaagcg ttggaaatta caagattcaa
    20041 acttaataag gtatgtttaa tacgaagtca acactaagtt agcatgactc acgccgggcg
    20101 tccagttttt agcgacgggg cacccgaaca tgaaattccc ttacgccagg ttagcgtcga
    20161 ggaactcttt caactgacct ttagacagtg cgcccacttt ggttgccgcc acttcaccgt
    20221 ttttgaacag cagcaaagtc gggataccac ggatgccata tttcggcgca gtgccagggt
    20281 tttgatcgat gttcagtttt gcaacggtca gtttgccctg atattcgtca gcgatttcat
    20341 ccagaatcgg ggcgatcatt ttgcacggac cgcaccactc tgcccagaaa tcgacgagga
    20401 tcgccccgtc cgctttgagt acatccgtgt caaaactgtc gtcagtcagg tgaataattt
    20461 tatcgctcat atataactcc acaggaataa gcctggcgtg ttggtttcgt tgttggtgta
    20521 acattaacca actaaaggtt gactttattt caccggatac gctttcgtaa agcaatagta
    20581 agctgatatt ctaccacact atgagcaaaa cacatttaac agaacagaag ttttccgact
    20641 tcgccctgca tccgaaggtt gtagaagccc ttgaaaaaaa agggtttcat aactgtacgc
    20701 ccattcaggc actggccctt ccgctgacgc tggcgggtcg tgacgtagcc gggcaggcgc
    20761 aaaccggtac cgggaaaacg atggcgtttc ttacgtcaac gtttcattat cttctctctc
    20821 atcccgcgat tgccgatcgc aaggtgaatc aaccgcgtgc cttaattatg gcaccgacgc
    20881 gtgaacttgc cgtgcagatc cacgccgacg cggagccact tgcgcaagct accggcctga
    20941 agctgggtct ggcttacggt ggtgatggct acgacaaaca gctgaaagtg ctggaaagcg
    21001 gcgttgacat tctgattggc accacggggc gtttaattga ctacgccaag cagaaccaca
    21061 ttaacctcgg tgccattcag gtagtggtac tggacgaagc cgatcgtatg tacgatctgg
    21121 gctttattaa agatatccgc tggctgttcc gccgtatgcc gcctgcaaac cagcgcctca
    21181 acatgctgtt ctccgccacg ctttcgtacc gggtacgtga actggcgttc gagcagatga
    21241 acaatgccga atatattgaa gtggaaccgg aacagaaaac gggccaccgt attaaagaag
    21301 agcttttcta cccttctaac gaagaaaaaa tgcgtttgct gcaaacgctg atcgaagaag
    21361 agtggccaga cagagcgatt attttcgcca acaccaaaca ccgttgtgaa gagatctggg
    21421 gccacctggc ggcagatggt catcgtgtcg gtttgttgac aggcgatgtc gcgcagaaaa
    21481 aacgtctgcg tattcttgat gaatttaccc gtggtgattt ggatattctg gttgccaccg
    21541 atgttgccgc gcgtggcctg catatcccgg ctgtgacgca cgtctttaac tacgatttac
    21601 ccgatgactg tgaagattac gttcaccgta ttggtcgtac aggtcgcgct ggcgcaagtg
    21661 gtcattccat cagcctggca tgtgaagagt atgcattgaa tttgcctgct attgagacct
    21721 atattggtca ctcaattccg gtaagcaaat acaatccgga cgcattgatg accgatctgc
    21781 caaaaccgct gcgcctcacg cgcccgcgta caggcaatgg tccgcgtcgt actggtgctc
    21841 cgcgtaatcg tcgtcgttca ggttaaataa aatgtcatac tcaaaatcat tcaaactgta
    21901 tcaagatggc aagagaatga atcccgatgc gcatgctgta gtaagtgatt tgggtgaaag
    21961 aacgcagcca acacagagac agattgaagg atgaagagta tgggttccac ctcgtcgctg
    22021 tatgcagcca ttgatctcgg ttcgaatagt tttcatatgc tggttgtgcg cgaggtggct
    22081 ggaagcatcc agacgctgac gcgaattaaa cgcaaagtgc gtctggctgc tggcctgaat
    22141 agcgaaaatg ccctgtctaa tgaagcaatg gagcgcggtt ggcaatgtct gcgcctgttt
    22201 gctgaacgtc tgcaagatat ccctccctcg caaattcgcg ttgtcgctac ggcgacatta
    22261 cgactagccg tcaatgcggg tgattttatc gccaaagcac aggaaatcct tggctgcccg
    22321 gtacaagtca tcagcggtga agaagaagcc cgcctgattt atcagggggt ggcccacaca
    22381 acaggcggtg ccgatcagcg cctggtggtg gatataggcg gtgccagtac tgaactggta
    22441 accggcacgg gtgcacaaac cacctcgttg ttcagcctgt cgatgggctg cgtcacctgg
    22501 ctggaacgct attttgccga tcgtaatctg gcgcaggaaa attttgatgc tgcagaaaaa
    22561 gcggcacgcg aagtgttacg tccggttgca gatgaattac ggtatcacgg ctggaaagtg
    22621 tgcgttggcg cttccggcac cgtgcaggcg ttacaggaaa tcatgatggc acaggggatg
    22681 gatgaacgca ttaccctgga aaagttgcag caactgaaac aacgcgccat tcattgcggt
    22741 cggctggaag agctagagat tgacgggctg acgctggaac gtgcgttagt gttcccgagt
    22801 ggtctggcga tcctgatagc catttttacc gaactgaata ttcagtgtat gaccctggcg
    22861 ggcggtgcgc tgcgtgaagg cctggtctac ggtatgttgc atcttaccgt cgagcaggat
    22921 attcgcagcc gtacgctgcg taatattcag cgccgcttta tgatcgatat tgatcaggca
    22981 cagcgcgtag ccaaagttgc ggctaacttc tacgatcagg tggaaaatga atggcatctt
    23041 gaagcaataa gccgcgattt gctcatcagc gcctgtcagc ttcatgaaat cggcctgagc
    23101 gttgacttca aacaagcgcc gcaacacgct gcttatctgg tacgtaatct ggatcttccc
    23161 ggttttaccc ccgcacagaa aaaactgctg gcgacgctac tgctcaacca gactaatccg
    23221 gtcgatctct catcgctgca tcagcaaaat gccgtaccac cgcgcgtcgc agaacaactc
    23281 tgccgtttac tccgcctggc tatcattttt gccagccgtc gccgtgacga tctcgtgcca
    23341 gagatgacat tacaggctaa ccatgaactg ttgaccttga cgcttccgca aggttggcta
    23401 acccaacatc cgctgggtaa agagattatt gatcaggaaa gccagtggca gagctatgtc
    23461 cactggccgc tggaagtgca ttaatgagta agtgccggat gcgatgctga cacatctttt
    23521 ccggccttga ttatttccct cgttttgccg ccatcatcgc tttcagattc gccagatggc
    23581 tttgcccttt ctgcatccgt tcttcggcgc tgaccacttt gcgctcctgt tcccaaatca
    23641 aatcatcctg cggcagctcc agcaggaagc ggctcggctc cgggcgcacc agttctccgt
    23701 actgacggcg ttctttacac agcgtaaagg tcaattcctt ctgggcgcgg gtaatgccga
    23761 cataggccag ccgccgctcc tcatcgatat tatcttcatc gatgctgctc tggtgcggca
    23821 aaaacccctc ttccataccg accatgtaga cataaggaaa ctccagccct ttcgacgcgt
    23881 ggagagtcat cagttgcacc tgatccagct cttcttcact ctcaccacgc tccatcatgt
    23941 cgcgcaaagt aaagcgcgtc accacctggg tgagcgtcat cggctcatcc agttcgctgc
    24001 cttccagcat ctccgtcatc cagctaaaca gttggttgac gttcttcatg cgcatttcgg
    24061 cggctttcgg gctgggcgat gtttcgtaca gccaggattc ataatccatg ccgtggatca
    24121 gatcacgcac cgcggcaatc ggctcccgct ccgccagacg ctggatttct gccaaccagt
    24181 gagtgaagcg ggtcaatgct tcataaccac gtccgctaag cgtctgactc aggcccatat
    24241 caaagctggc ggtaaacatg cttttattgc gcgtcatcgc ccattcaccc aactttttca
    24301 gcgtcgccgg gccaatctct cgcttcggcg tgttaacgat acgcaggaac gcactatcat
    24361 cgtccgggtt agtcagcacg cgcagataag ccagcaagtc cttgatttca ggacgagaga
    24421 aaaacgacgt accaccagaa attttgtacg ggatgcggtt ttgcatcagg aatttttcaa
    24481 acacccgcga ctgatggtta ccgcgataaa gaatggcgta atctttgtac tgcgttttat
    24541 tgacgaagtg atgggcaatc agctcgccag taacgcgctc ggcttcatgt tcctcgttat
    24601 tcgcgcttaa aacttttagc tctgtgccgt aacccagttc ggagaacaga cgcttttcaa
    24661 agacgtgcgg gttattggcg atcaggatgt tcgccgcttt caaaatacgt ccggaagagc
    24721 ggtagttctg ctcaagctta atcaccttca gcgccggaaa atcctgactc agcagcacca
    24781 ggttttgcgg acgtgcgccg cgccaggagt agatcgactg gtcatcgtca cccaccacgg
    24841 taaagcgcgc gcggctgccc accagcagtt tcaccagctc atactggctg gtgttggtgt
    24901 cctgatattc gtccaccagc aggtagcgaa ttttgttctg ccagcgctcg cggacttctt
    24961 cattgcgttg caacagcaac gttggcaata aaatcagatc atcgaaatcg agaacgttac
    25021 aggctttcag gtgtgcatca tacagcccat aacaatgggc aaaaatacgg tcccgctcgc
    25081 cctttgcctc tgccgccgcc tgcgccggtg ttttgagatc gttcttccag ttggagatgg
    25141 tcgaaattag ctgttgcagg agaactttgt catcttcaat cagcccctcg gtcaactctt
    25201 taagcaaagc aagctgatcg gtatcgtcaa acagcgaaaa gttagccttc atccccaacg
    25261 ccgcgtactc acgcttaatg atatccagcc ccagcgtgtg aaaggtggag atcatcagcc
    25321 cacgcgcctc tttacgcccc agcgtctgcc cgacacgctc tttcatctcg cgtgccgcct
    25381 tattggtaaa ggtcaccgcc gcgatatgtc gcgcctgata accgcaaccg cggatcagat
    25441 gggcgatttt attggtgata acacgagttt taccggaacc cgcgcccgcc agcaccaggc
    25501 aggggccggt aacgaattcg acagcttgtt gttggccggg gtttagacgc ataggaaaat
    25561 cgctcaatga aagtcgggag gggaaaataa cagcgtggta gtatagccag cctcatcctt
    25621 accactcaag gcacgatcat ggcaaaaaca gcagcagcac tgcatatcct tgtaaaagaa
    25681 gagaaactgg ctctggatct tctcgagcag atcaagaatg gtgccgattt cggcaagctg
    25741 gcgaagaaac actccatttg cccgtcaggc aaacgcggcg gtgatttggg tgaattccgc
    25801 cagggtcaaa tggttccggc gttcgataaa gtggttttct cttgtccggt actggagcca
    25861 accggcccgc tgcataccca gttcggttac cacatcatta aggtgctgta ccgtaactaa
    25921 cagcaaggcc ttctccggga gaaggccttg aactctgaat cgctattaac ccgcaacagc
    25981 aatacgtttc atatccgtca tatagccgcg cagtttctta cctacctgct caatcgcatg
    26041 gctgcgaatc gcttcattta catcacgcag ctgcgcgtta tctaccgcac cttccggaat
    26101 agctttaccc aggtcgcccg gttgcagctc tgccataaac ggtttcagca gcggcacaca
    26161 agcgtaagag aacagatagt taccgtactc ggcagtatcg gagataacca cgttcatttc
    26221 gtacagacgc ttacgggcga tggtgttggc aatcagcggc aattcgtgca gtgattcata
    26281 gtaagcagat tcttcgatga tgccggaatc aaccatggtt tcaaacgcca actcaacgcc
    26341 tgcttttacc atcgcgatca tcagtacgcc tttatcgaag tactcctgtt cgccgatttt
    26401 gccttcatac tgcggcgcgg tttcgaatgc ggttttgcca gtctcttcac gccaggtcag
    26461 cagtttctta tcgtcgttgg cccagtcagc catcatgccg gaggagaatt cgccggagat
    26521 gatgtcgtcc atatgtttct ggaacagcgg cgccatgatc tctttcagtt gctcagagag
    26581 cgcgtaagca cgcagtttcg ccgggttaga aagacggtcc atcatcaggg tgatgccgcc
    26641 ctgtttcagc gcttcggtga cggtttccca accgaactga atcagttttt ctgcgtatgc
    26701 cggatcggtg ccttcttcca ccagcttgtc gaagcacagc agagaaccag cttgcaacat
    26761 accgcacagg atggtttgct cgcccatcag gtcagatttc acttccgcaa cgaaagagga
    26821 ttccagcacg cccgcacggt gaccgccggt tgcagccgcc catgctttag cgatcgccat
    26881 gccttcgcct ttcggatcgt tttccgggtg aacggcaatc agcgtcggta cgccgaatcc
    26941 acgtttgtac tcttcacgta cttcggtgcc agggcatttc ggcgcaacca ttacgacggt
    27001 gatgtcttta cggatctgct cgcccacttc gacgatgttg aaaccgtgtg agtaacccag
    27061 cgccgcgccg tctttcatca gtggctgtac ggtgcgtact acatcagagt gctgcttgtc
    27121 cggcgtcagg ttaaccacca gatccgcctg cgggatcagt tcttcgtaag tacccacttt
    27181 aaaaccattt tcggttgctt tacgccagga tgcgcgcttc tcggcaatcg cttctttacg
    27241 cagagcgtaa gaaatatcca gaccagaatc acgcatgttc aggccctggt tcagcccctg
    27301 tgcgccacag ccgacgatga ctactttttt accctgaagg tagctcgcgc catcggcgaa
    27361 ttcatcgcgc cccataaagc gacatttgcc cagctgtgcc agctgctggc gcagattcag
    27421 tgtattgaag tagttagcca tggtgattcc tcgtgatgtt gtgcttctta ttgttcggtt
    27481 cgcgtttgcg agattgaatt cactatatga caggaaattt attgcggaaa ttgatatatt
    27541 cacaacgtca cattgcaatt tctgcaacgt caacattgag ggctgcgcca ttggatttac
    27601 gcgatctgaa aaccttcctg catctggcgg aaagccgcca ttttggccgt agcgcgcggg
    27661 caatgcacgt tagcccgtct acgctttcgc gacaaataca gcgcctggaa gaagatctcg
    27721 gtcagccgct gtttgtgcgt gataaccgca cggtaacgct gacggaagcg ggcgaagagc
    27781 tgcgcgtttt cgcccagcaa acgctgttgc agtatcagca attgcgccac accatcgacc
    27841 agcaagggcc gtcgctctct ggcgaattac atatcttctg ctcggtgacc gctgcctaca
    27901 gccatctgcc gccgattctg gatcgcttcc gcgcggaaca tccgtcagtg gagatcaaac
    27961 tctctaccgg tgatgcggcc gatgcgatgg aaaaggtggt cactggcgaa gcggatctgg
    28021 cgattgccgg gaaaccggaa accttgccgg gcgcagtggc gttttcgatg ctggagaatc
    28081 tggcggtggt gctgattgct cccgcgctgc cctgcccggt gcgtaatcag gtatctgtcg
    28141 aaaagcccga ctggtctaca gtgccgttta tcatggccga tcagggaccg gtacgtcgcc
    28201 gcattgaact gtggtttcgg cgtaacaaaa tcagcaaccc aatgatttac gccacggttg
    28261 gcgggcatga agcgatggta tcgatggtgg cgctcggctg tggcgtggcg ctgttgccgg
    28321 aagtggtgct ggataacagc ccggaaccgg tgcgtaaccg cgtgatgatt ttagagcgca
    28381 gtgatgaaaa aacgccgttt gagctgggcg tgtgcgtaca aaaaaagcgg cttcatgagc
    28441 cgctgattga ggcgttctgg aagattttgc cgaaccacta acccgccaaa aagaacctga
    28501 acgccgggtt attggtttcg tcgtggcaat cgtagcccag ttcattcagc cgggtttcaa
    28561 aatccggttc atgatcgcca agctcgaacg ccgccagtac gcgcccgtag tcggtaccgt
    28621 ggctgcgata atggaacagc gagatgttcc agtgcgtacc cagcgtgttg aggaagcgca
    28681 gcagcgcgcc cggcgattcc gggaattcga agctgtagag gcgttcctgc aacggatgcg
    28741 atggacgccc gccaaccata tagcgcacat gcagcttcgc catttcgtcg tcggagagat
    28801 ccaccacgct gtagccgcca tcgttgagca tctgcaaaat ttctttgcgc tcttcgaggc
    28861 cacggctcag gcgcacgccg acaaaaatgc aggcgttttt ggcatcagca aagcggtagt
    28921 tgaactcggt gaccgagcgc ccaccaagta actggcagaa tttgaggaag ctgccctttt
    28981 cttccggaat ggtcaccgcc agtaacgctt cacgctgttc gcccagttcg cagcgttccg
    29041 agacgtagcg cagaccgtga aagttcacgt tggcaccgga aagaatatgc gccagccgct
    29101 caccgcgaat gttgtgctgg gcgatgtatt ttttcatccc tgccagcgcc agcgcgccag
    29161 agggttcagc caccgcgcgt acatcttcga acaggtcttt catcgccgca cagatggcat
    29221 cgctatcgac ggtgatgatg tcgtcaagat actcctggca caaacggaag gtttcatcgc
    29281 cgatgcgttt taccgcgacg ccttcagcaa acagccccac gcggggcaga tcaacgggat
    29341 gacctgcatc aagtgccgct ttcaggcagg cggaatcttc cgcttctacg gcgatcactt
    29401 tgatttgtgg catcagttgt ttgatcagca ccgccacgcc cgccgccaga ccgccgccgc
    29461 cgaccggaac aaatacgcgg tcgagatgag catcctgctg gagcagttcc agcgccagcg
    29521 taccctgacc agcgatcacc atcggatgat cgaagggtgg cacccaggtg aagccctgct
    29581 gctgtgacag ttcgattgct ttcgctttcg cttcgtcaaa gttcgcgcca tgaagcagca
    29641 cttcgccgcc aaagccgcgc accgcatcaa ctttgatatc ggcggtggcg gttggcatga
    29701 cgatcagcgc cttcacgcct aaccgtgcgg aagaaaacgc gacgccctgc gcgtggttac
    29761 ccgcagaagc ggtaatcacg ccgtgcgctt tttgttcttc cgtcaggccc gccatcatgg
    29821 cgtatgcacc acgaagctta aagctgtgca ctggctggcg atcttcgcgc ttcaccagaa
    29881 tcacgttatc aagacgcgac gacagttttt ccattttctg tagcggcgtg acctgcgccg
    29941 cttcgtagac cggtgcgcgc agcaccgctc ttaaatattc ggcaccttcc ggggcaccgg
    30001 acaggggttg tgagtctgcc atcattaacc ccccagtttc gatttatcgc gcaccgcgcc
    30061 tttgtcggcg ctggttgcca ggctggcgta ggcgcgcagg gcaaaggaga cctgacgttc
    30121 acggtttttc ggcgtccagg ctttgtcacc ccgagcttcc tgcgcttcac gacgcgccgc
    30181 cagttcggca tcgcttacct gtaactgaat accacggttc ggaatgtcga tggcgatcag
    30241 gtcgccgtct tcaatcaggc caatgctgcc gccgcttgcc gcttccggtg agacgtggcc
    30301 gatagaaagg ccagaggtac cgccagagaa acgaccgtcg gtgatcagcg cacaggcttt
    30361 accgagaccc attgatttca ggaagctggt tgggtagagc atttcctgca tccccggacc
    30421 gcctttcggg ccttcatagc gaattactac cacatctccg gcgacaactt taccgccgag
    30481 aatcgcttct accgcatcgt cctggctttc gtacactttc gccgggccgg tgaatttgag
    30541 gatgctgtca tccacgcccg cggttttaac gatgcagccg ttttccgcaa agttaccgta
    30601 gagcaccgcc aggccgccgt ctttgctgta ggcgtgttcc agcgagcgga tacagccatt
    30661 ggcgcgatcg tcgtccagcg tatcccaacg gcaatcttgc gagaatgcct gggtggtacg
    30721 gatacccgca gggccggcgc ggaacatatt ttttaccgcg tcatcctggg tcagcataac
    30781 gtcgtattgt tccagcgttt gcggcaacgt caggccgagc acgtttttca catcacggtt
    30841 cagtaacccc gcgcgatcca gttcgccgag aataccgata acaccaccag cacggtgaac
    30901 atcttccata tggtatttct gggtgctcgg cgcaacttta cacagctgtg gaaccttgcg
    30961 ggaaagctta tcgatatcac tcatggtgaa gtcgatttcc gcttcctgcg ccgccgccag
    31021 cagatgaagt acggtgttgg tagagccgcc catcgcgata tccagcgtca tggcgttttc
    31081 aaacgccgcc ttactggcga tattacgcgg cagtgcacta tcgtcgtttt gctcgtaata
    31141 acgtttggtc aattcaacaa tgcgtttacc agcattgagg aacagctgct tacggtccgc
    31201 gtgggttgcc agcaacgagc cgttgcccgg ctgcgacagg cccagtgctt cggtcaggca
    31261 gttcattgag ttagcagtaa acatcccgga gcaggaaccg caggttgggc aggcggatcg
    31321 ttcaacctga tcgctctggg agtcagaaac tttcgggtct gcaccctgga tcatcgcatc
    31381 aaccagatcg agcttaatga tctgatcgga cagtttggtt ttcccggctt ccatcgggcc
    31441 gccggaaaca aagatcaccg gaatattcag gcgcagggaa gccatcagca tccccggggt
    31501 gattttgtcg cagttagaga tgcagaccat ggcgtcggcg cagtgagcgt tgaccatata
    31561 ctcaacggaa tcagcgatca attcgcgaga tggcagtgaa taaagcatcc ccccgtggcc
    31621 catggcaatc ccatcatcca ccgcaatggt gttgaactct ttggcaacgc cgccagccgc
    31681 ttcaatttgt tcggcgacca gtttaccgag atcgcgcaga tggacgtgac ccggtacaaa
    31741 ttgggtgaac gagttcacaa ccgcgataat cggcttaccg aaatcggcgt cggtcattcc
    31801 ggtggcgcgc cacagcgcac gagcacccgc catattacga ccatgagtgg tggtggcgga
    31861 acggtactta ggcatacttt atttactccc agtgtctgtc tcgtaaatgg gacggtgcgt
    31921 gccgtcccat tttttgtatt tattgattaa cttgatctaa ccagccccat ttatcttcgg
    31981 tttcgccagt gaagaggccg aagaaggctt gctgaatgcg tttggtaacc gggccgcaac
    32041 ggccttcacc aacctgaata ccgtctacac tgcgcactgg cgtgatttct gccgcagtac
    32101 cggacataaa cacttcatcc gccaggtaca gtgattcgcg cgacagcacc tgctcacgca
    32161 cttcaattcc cagctctttc gccagtttga tgatggcgtc acgggtaata cccggcagcg
    32221 cggatgaggt gaacggtggg gtgaacagca caccgtcttt cacttcaaac aggttttcgc
    32281 ctgcgccttc agagatgtaa ccgttcacat ccagcgcgat accttcctga taaccgtggc
    32341 ggcgtgcttc actacccacc agcagggaag agaggtagtt accaccggct tttgccgcgg
    32401 ttgggatggt gtttggtgct gcgcggttcc aggaggaaac catcgcatcg atcccctgct
    32461 ccagcgcttc tgcgcccaga tacgcccccc acgggaaagc ggcgataatc acgtcggttg
    32521 agtatcccgc tggcgggttt acgcccatgc caacatcacc aacgaagatc agcggacgga
    32581 tataggcgct ggtgagattg tttttgcgga tcacgtcacg acaagcttcc atcagctcat
    32641 caatgctctg cgaaaccggg aagcgataga ttttggcgga gtcatgcaga cgctgcatat
    32701 gctcacgatg gcggaataca accggtcctt tgtgcgagtc gtagcaacgg atgccttcaa
    32761 aaaccgaggt gccatagtgc agcgcgtgcg acatcacatg caccttcgcg tcttcccagc
    32821 gaaccatctc cccattgaac caaatgtaat cagctttctt cgtggtcatt tttatattcc
    32881 ttttgcgctc aggcgcggat ttgttgtgat gtggttgtgc tctggcagat ggcaacgtgt
    32941 gcgacgtcca ccagtttatt taactgacta aacagtaagt cgaccgaccg tgggctggca
    33001 acggtcaatt cgatatttat attttgtgca tcgctggcgg cggccatatt cattgaacag
    33061 acgtggaaac cacgatgacg caccacgcgt aaaacacgtt ctaaggtttc cggattgaag
    33121 cgagccgata cattgacctg atgttgcatc atgataattt ctccaacatt tctgaattac
    33181 tggcaccagg cggcaccagc ggccagacgt tctcaagttc gtcgattgag acatgaagca
    33241 ggtatggccc atcactgttc agcatggtgt cgagtgccgc ttcaacctgg tctttatggg
    33301 tgatgtgttg gccagggatg ccgaaggcgc tggctaacat gaggaaatcg gggttatcgg
    33361 taagggtggt ttcgctgtat cgttcctgga aaaacagttg ctgccattgt cgaaccatcc
    33421 ctaaccgttg gttatcgagt aagacgattt tcaacggtaa ctgcttgcgt tttacggtgc
    33481 ccagctcttg cacattcatc atgaaagagc cgtcaccgga gatacagacg acagtatcgt
    33541 tcggtcgcgc gacttgtgcg ccaaccgccg ccggtaaacc gaaacccatg gtgcctaagc
    33601 cgctggaggt gatgaagttt tccgggcgag tgtggacgat gtgctgggca gcccacatct
    33661 ggtgctgccc cacatctgtg gtcacgatgc aatccgcaga tttacgatcc gacagttgtt
    33721 ttaacaacaa cggcgcgtag atagcgtcac caggatggtc gtaacgccag gcatgttcat
    33781 cacgcagttg cgcgcagtgt tgctgccagt catcgatatt taacggctgc tgtaatgctg
    33841 gtaacagagc atttaaatca ccctgtaatg ccacatgtgc ctgacgcagc ttgttcattt
    33901 ctgccggatc gatatccata tggataacgc tggcgtgtgg tgcgaaggtg ttcagtttgc
    33961 cggtcacccg gtcatcaaaa cgtgcgccca cggcgatcag tagatcgcac tcctgcactg
    34021 cgaagttcgc cgctttggtg ccgtgcatcc ccagcatgcc cagatagtac ggataatctg
    34081 cttctactgc gcccagccct ttcaacgtac aggtggcagg catttttgtg gcagcgagaa
    34141 attcacgcaa agccgaaact gcctgcgcca tgcccacgcc accaccaacg tacagcatcg
    34201 gtttttgcgc ttttgccagc atctgacgcg cttgctcgac ttcggcatgt gggaaagtca
    34261 cttcgttttc aacagtggtg aaccacggtt ccagatcgcc gctggctaac tggatatctt
    34321 ttggaatatc gaccaaaacc ggaccaggac gaccagagct ggcgacgtca aacgcttcgg
    34381 ccatgatacg cggtaactct tccagtgatt gcaccaggaa actgtgcttg gtgcaggcta
    34441 acgacaaccc cagaatatcc acctcctgaa atgcgtcagt gccgataaac ggtgcggata
    34501 cctgaccggt gatagcaaca acggggatgg aatctaacag cgcgtccgca agcccggtta
    34561 tcaggttggt tgcacctgga ccagacgtgg cgatgcaaac gcccgttttg ccggtagcac
    34621 gggcataacc gatagccgcc attgctgcac cttgctcgtg tcggcacagc aagtgttcca
    34681 cgccaccgtc atacaatgca tcgtaaaccg gcataattgc gccacccgga taaccgaaaa
    34741 cggtgttcac accctgtgcc cgcaacgcat gtaccaccca ctgtgcgcca ttcatagtta
    34801 gttccccgtc ctgaatcttg agaaacagaa ttttgtgctg ttattcattg tctgctcctc
    34861 agttatgttt ttaaggtcaa aaaaaacccc cggacctttc ggtgcggggg tcttagttcg
    34921 ttaaggcttg atctctaagc ctttcctcgt ccaagtgcag ccccgcacgg tgggataata
    34981 atcaccacca cgctaatcac gaccaggcta atcactcgta gaagggctgt cattttgtct
    35041 tttcttgcat cttgttcgaa ggaatgccta aagagttacc atagattttg cgaatagcac
    35101 aagatatttt ttggccgatt tttatgatgt ctttaataat ttcaagagaa attgttgttt
    35161 tttaaaaaga aaatagtgag tgaaaaaaat tcaatttcag tatgaatgtt gttcccttcc
    35221 tcgtagttca tcctttgccc gctgcatttt tgcgagcatc attccaccgc cagaacaact
    35281 caacttcact tgcacaaata aacaggaatc agccactaaa attcacgaat tagcgggcta
    35341 cagcagcgat atcttatgca cataatggtg actcaaggag ggcttatgtc actgtcaatt
    35401 gttcataccc gcgcagccct gggagtaaat gcgcccccaa tcactgttga ggtacatatc
    35461 agtaaaggtc taccaggctt aacgatggtg ggcttaccag aaacaacggt aaaagaagct
    35521 cgcgatcgcg tgcgcagcgc cattatcaat agcggatatg aatatccggc gaaaaaaatc
    35581 accatcaacc ttgctccagc cgatctgcca aaagaagggg gacgatatga tttgcctatc
    35641 gctattgcgt tactggcggc ctcagaacag cttacagcca ataagttaga tgaatatgaa
    35701 ttagtcggag aactggcgct tacaggcgct ctgcgtggcg ttcccggcgc aatctccagt
    35761 gcaactgaag ccattaagtc gggcagaaaa attatcgtcg cgaaagataa cgaggatgaa
    35821 gtggggctaa ttaacggtga aggatgcctg atagccgatc atctgcaggc tgtctgtgcg
    35881 tttctggagg gtaagcacgc tctcgaacgc ccgaaaccaa ctgatgcagt atcccgggcg
    35941 ctacaacatg atctcagtga tgttgtcggt caggagcaag gaaagcgagg actggaaatt
    36001 accgccgctg gcgggcacaa ccttttactg attgggccgc cgggaacagg taaaacaatg
    36061 ctcgccagcc gtattaatgg cctgttgcca gatttaagca atgaagaggc actggagagc
    36121 gctgcgatat taagtctggt aaatgctgaa tcagtacaaa aacaatggcg gcagcgccca
    36181 ttccgctcac ctcatcacag tgcgtcgtta actgcgatgg tgggcggtgg tgcaattcca
    36241 gggccaggtg aaatttcgct ggcgcataac ggcgtgcttt ttcttgatga gctacctgaa
    36301 tttgaacggc gtacactgga tgccttgcga gagccgattg aatccgggca gatccatctt
    36361 tcacgcacac gagcaaaaat aacctatcca gcccgtttcc aacttgttgc ggcgatgaat
    36421 cccagcccta ccggacatta tcagggaaac cataaccgct gcacaccaga acagacatta
    36481 cgttatctca accggctctc ggggcccttt ctcgaccgct tcgatctctc actagagatc
    36541 ccattaccgc cccccggcat tttgagtaaa acggtagtgc agggagaaaa cagcaccacc
    36601 gttaaacaac gtgtaatggc cgccagagag cgccaattta agcggcagaa taaactgaac
    36661 gcctggctgg ataatccgga aatacgccaa ttctgcaagc ttgagagcga agatgcgcag
    36721 tggctggaag aaacgctgat ccatctgggg ttatcgattc gtgcctggca gcggttattg
    36781 aaagttgcac gaaccattgc tgatattgat cagtctgaca ttatcacacg tcagcatttg
    36841 caggaggcag ttagctatcg tgcgattgac cgtttgctca tccatctgca aaaactactg
    36901 acataaaaaa aagggcattt cgcccttttt attaatcgtc agaatcggtg tagtcttcag
    36961 caccttcaac ctgcggttta ccgccggaaa gggtgtgaaa acgttttgga cgcttgatac
    37021 gcgtcatata cttggaccac acgcgttctg cttctgtcac tggctcacgt tcgccacggc
    37081 atactgctac gaagagtttc tcttcctcgg taaccggctc gcgtttgcca agatccaact
    37141 cattgaaggc ataaccatga cgctcaagca gttgtgcctc tttgatggtg aagtcaccat
    37201 gacgagagaa tccacgtgga taatgtttat tgtcgaaata tcgattagtc gtcgtaaagc
    37261 tttccgccat cctgcacgct cctaattctt tgaccgagct agttatggcg cggagtatta
    37321 gttacgcttg acagagtgta aaacaaaaca tttaaatcat aacgacaaat aattttgcgg
    37381 agagcactgt ggatacggaa ttgttaaaaa ctttcctgga agttagccga acgcgtcact
    37441 ttggtcgagc agctgaatcg ctctatctga cccaatcagc cgtgagcttt cgaatcagac
    37501 aactggaaaa tcaactgggt gtgaaccttt tcacccgcca cagaaacaat atccgtttaa
    37561 ccgctgctgg tgaaaaacta ctgccttatg cagaaacgct catgagcacc tggcaggccg
    37621 cccgtaagga ggtggcgcat acctcacgac ataacgagtt ttctatcggt gccagcgcct
    37681 cgttgtggga atgtatgctt aatcagtggc tgggacgctt gtatcaaaat caggatgtcc
    37741 atacaggctt acagttcgaa gcgcgaattg cccaacggca gtctctggta aaacagctgc
    37801 atgaacgcca gcttgatctt cttattacta ctgaagcgcc caaaatggac gaatttagca
    37861 gtcagttgct cgggtatttc actttagcgc tttataccag tgccccttct aaactaaagg
    37921 gagatcttaa ctatctgcga ctggaatggg ggccagattt tcaacagcat gaggcaggtt
    37981 tgatcggtgc tgacgaagtt cccattctga caaccagttc tgctgaactg gcacagcaac
    38041 agattgcgac gcttaatggt tgcacctggc tacccgtcag ttgggcgcgt aaaaagggcg
    38101 gcctgcatac cgttgtcgat agcacaacac tttcacggcc gctttatgcc atatggctgc
    38161 aaaatagcga taaaaatgcg ttgattcgcg atctattgaa aattaacgtg ctggatgaag
    38221 tgtattaatc tgaatggctg gcaaggatgc cggtagaagg atttacttcg gagaggttat
    38281 ttcagataaa aaaaatcctt agctttcgct aaggatgatt tctggcaggg gcggagagac
    38341 tcgaactccc aacacccggt tttggagacc ggtgctctac caattgaact acgcccctaa
    38401 ttagggtggc ggaacggacg ggactcgaac ccgcgacccc ctgcgtgaca ggcaggtatt
    38461 ctaaccgact gaactaccgc tccaccgaat tcttttacaa ccaccggttt tatgaccggc
    38521 ttactgctta atttgatgcc tggcagttcc ctactctcgc atggggagac cccacactac
    38581 catcggcgct acggcgtttc acttctgagt tcggcatggg gtcaggtggg accaccgcgc
    38641 tacggccgcc aggcaaattc tgtttcatca gaccgcttct gcgttc
//
LOCUS       DAZGQL010000044        38068 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.044, whole genome shotgun sequence.
ACCESSION   DAZGQL010000044 DAZGQL010000000
VERSION     DAZGQL010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38068
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.044"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(74..769)
                     /gene="aqpZ"
                     /locus_tag="ACHKIR_002490"
     CDS             complement(74..769)
                     /gene="aqpZ"
                     /locus_tag="ACHKIR_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HFO9990216.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAAAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRN"
     gene            1195..2853
                     /gene="ybjD"
                     /locus_tag="ACHKIR_002491"
     CDS             1195..2853
                     /gene="ybjD"
                     /locus_tag="ACHKIR_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HFO9990217.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(2850..3806)
                     /gene="ybjX"
                     /locus_tag="ACHKIR_002492"
     CDS             complement(2850..3806)
                     /gene="ybjX"
                     /locus_tag="ACHKIR_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HFO9990218.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYKGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            3957..5072
                     /gene="macA"
                     /locus_tag="ACHKIR_002493"
     CDS             3957..5072
                     /gene="macA"
                     /locus_tag="ACHKIR_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019898 - extrinsic component of membrane
                     [Evidence IEA];
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HFO9990219.1"
                     /translation="MKKRKTVKKRYVIALVIIIVGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDNAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKINDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            5069..7015
                     /gene="macB"
                     /locus_tag="ACHKIR_002494"
     CDS             5069..7015
                     /gene="macB"
                     /locus_tag="ACHKIR_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HFO9990220.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGISLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTASGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTVT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(7088..7312)
                     /gene="cspD"
                     /locus_tag="ACHKIR_002495"
     CDS             complement(7088..7312)
                     /gene="cspD"
                     /locus_tag="ACHKIR_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HFO9990221.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            7462..7536
                     /gene="yljB"
                     /locus_tag="ACHKIR_002496"
     CDS             7462..7536
                     /gene="yljB"
                     /locus_tag="ACHKIR_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YljB"
                     /protein_id="HFO9990222.1"
                     /translation="MNQKFEAVNAIDRNVTDVADANDR"
     gene            7635..7955
                     /gene="clpS"
                     /locus_tag="ACHKIR_002497"
     CDS             7635..7955
                     /gene="clpS"
                     /locus_tag="ACHKIR_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HFO9990223.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            7986..10262
                     /gene="clpA"
                     /locus_tag="ACHKIR_002498"
     CDS             7986..10262
                     /gene="clpA"
                     /locus_tag="ACHKIR_002498"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HFO9990224.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(10785..10872)
                     /locus_tag="ACHKIR_002499"
     tRNA            complement(10785..10872)
                     /locus_tag="ACHKIR_002499"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10836..10838),aa:Ser,seq:gga)
     gene            complement(11126..11344)
                     /gene="infA"
                     /locus_tag="ACHKIR_002500"
     CDS             complement(11126..11344)
                     /gene="infA"
                     /locus_tag="ACHKIR_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFO9990225.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(11629..12333)
                     /gene="aat"
                     /locus_tag="ACHKIR_002501"
     CDS             complement(11629..12333)
                     /gene="aat"
                     /locus_tag="ACHKIR_002501"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HFO9990226.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(12375..14096)
                     /gene="cydC"
                     /locus_tag="ACHKIR_002502"
     CDS             complement(12375..14096)
                     /gene="cydC"
                     /locus_tag="ACHKIR_002502"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HFO9990227.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAALEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFNYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNGSPIASLNEATLRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALAEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHNAPLVLLDEPTEGLDAT
                     TESQILELLAEMMHEKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(14097..15863)
                     /gene="cydD"
                     /locus_tag="ACHKIR_002503"
     CDS             complement(14097..15863)
                     /gene="cydD"
                     /locus_tag="ACHKIR_002503"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0042883 - cysteine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HFO9990228.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELASTDPVTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEHELQTALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGQIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(15986..16951)
                     /gene="trxB"
                     /locus_tag="ACHKIR_002504"
     CDS             complement(15986..16951)
                     /gene="trxB"
                     /locus_tag="ACHKIR_002504"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFO9990229.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLTGDSG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            17495..17989
                     /gene="lrp"
                     /locus_tag="ACHKIR_002505"
     CDS             17495..17989
                     /gene="lrp"
                     /locus_tag="ACHKIR_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HFO9990230.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            18124..22191
                     /gene="ftsK"
                     /locus_tag="ACHKIR_002506"
     CDS             18124..22191
                     /gene="ftsK"
                     /locus_tag="ACHKIR_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFO9990231.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDEE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQPQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPVQQPYYAPAAEQPVQQPYYATAAEQSAQQPYYAPA
                     PEQTAAGNAWQAEEQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQPVEQHPVVEPEPV
                     VEETKPARPPLYYFEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVP
                     PVEAAAAVSPLASGVKKATLATGAAATVAAPVFSLANSAGPRPQVKEGIGPQLPRPKR
                     IRVPTRRELASYGIKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFA
                     QTQQQRYGEQYQHDVPVNAEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFTLDDF
                     EFSPMKALLDDGPHEPLFTPIVEPVQQPQQPIAPQQQYQQPQQPVAPQPQYQQPQQPV
                     APQQQYQQPQQPVAPQPQYQQPQQPVAPQPQYQQPQQPVAPQPHDTLLHPLLMRNGDS
                     RPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGP
                     VITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTV
                     YLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAM
                     ILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMER
                     RYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAHHPVLKKEPYIVVLV
                     DEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAF
                     TVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKA
                     RGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIG
                     YNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            22350..22961
                     /gene="lolA"
                     /locus_tag="ACHKIR_002507"
     CDS             22350..22961
                     /gene="lolA"
                     /locus_tag="ACHKIR_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HFO9990232.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSNFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            22972..24315
                     /gene="rarA"
                     /locus_tag="ACHKIR_002508"
                     /gene_synonym="mgsA"
     CDS             22972..24315
                     /gene="rarA"
                     /locus_tag="ACHKIR_002508"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HFO9990233.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVDDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            24406..25698
                     /gene="serS"
                     /locus_tag="ACHKIR_002509"
     CDS             24406..25698
                     /gene="serS"
                     /locus_tag="ACHKIR_002509"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFO9990234.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            25937..28381
                     /gene="dmsA"
                     /locus_tag="ACHKIR_002510"
     CDS             25937..28381
                     /gene="dmsA"
                     /locus_tag="ACHKIR_002510"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HFO9990235.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSALPAKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLINQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGINNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDTKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            28392..29009
                     /gene="dmsB"
                     /locus_tag="ACHKIR_002511"
     CDS             28392..29009
                     /gene="dmsB"
                     /locus_tag="ACHKIR_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase iron-sulfur subunit
                     DmsB"
                     /protein_id="HFO9990236.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            29011..29874
                     /gene="dmsC"
                     /locus_tag="ACHKIR_002512"
     CDS             29011..29874
                     /gene="dmsC"
                     /locus_tag="ACHKIR_002512"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HFO9990237.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLIVTMVLGVIFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVMSVMQGAELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(29910..30536)
                     /gene="ycaC"
                     /locus_tag="ACHKIR_002513"
     CDS             complement(29910..30536)
                     /gene="ycaC"
                     /locus_tag="ACHKIR_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate family cysteine hydrolase YcaC"
                     /protein_id="HFO9990238.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            30851..31999
                     /gene="ycaD"
                     /locus_tag="ACHKIR_002514"
     CDS             30851..31999
                     /gene="ycaD"
                     /locus_tag="ACHKIR_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9990239.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITSMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            32209..33639
                     /gene="ycaM"
                     /locus_tag="ACHKIR_002515"
     CDS             32209..33639
                     /gene="ycaM"
                     /locus_tag="ACHKIR_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFO9990240.1"
                     /translation="MAGNVQEKQLRWYNIALMSFITVWGFGNVVNNYANQGLVVVFSW
                     VFIFALYFTPYALIVGQLGSTFKDGKGGVSTWIKHTMGPGLAYLAAWTYWVVHIPYLA
                     QKPQAILIALGWAMKGDGSLIKEYSVVALQGLTLVLFIFFMWVASRGMKSLKIVGSVA
                     GIAMFVMSLLYVAMAVTAPAITEVHIATTNITWETFIPHIDFTYITTISMLVFAVGGA
                     EKISPYVNQTRNPGKEFPKGMLCLAVMVAVCAILGSLAMGMMFDSRNIPDDLMTNGQY
                     YAFQKLGEYYNMGNTLMVIYAIANTLGQVAALVFSIDAPLKVLLGDADSKYIPASLCR
                     TNASGTPVNGYFLTLVLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAF
                     IAVVRLAQKYKPEYVFIRNKPLAMTVGIWCFAFTAFACLTGIFPKMEAFTAEWTFQLA
                     LNVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(33640..33750)
                     /locus_tag="ACHKIR_002516"
                     /pseudo
     CDS             complement(33640..33750)
                     /locus_tag="ACHKIR_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(33849..34589)
                     /gene="pflA"
                     /locus_tag="ACHKIR_002517"
     CDS             complement(33849..34589)
                     /gene="pflA"
                     /locus_tag="ACHKIR_002517"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HFO9990241.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMREVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(34781..37063)
                     /gene="pflB"
                     /locus_tag="ACHKIR_002518"
     CDS             complement(34781..37063)
                     /gene="pflB"
                     /locus_tag="ACHKIR_002518"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFO9990242.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(37118..37975)
                     /gene="focA"
                     /locus_tag="ACHKIR_002519"
     CDS             complement(37118..37975)
                     /gene="focA"
                     /locus_tag="ACHKIR_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HFO9990243.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
BASE COUNT         9390 a         9298 c        10139 g         9241 t
ORIGIN      
        1 gacgccacca acacccgtgc ggtttgtaac gtcgaagata aaacaaccag ggcagataaa
       61 atgccaatgc taattaatta cgcttttcca gcagggtccg gtaaatcaga ccaccgataa
      121 taccgccgac aattggcacc acccagaaga accacagttg ttctaacgcc cagccgccct
      181 ggaagatagc agccgcggtg ctgcgcgccg ggttaacaga agtgttagtc accggaatac
      241 tgattaagtg aatcagggtt aaggccagac caatagcgat cggcgcaaaa ccagcaggag
      301 cgaatttgtc ggttgcgccg tggatcacca gcaggaaacc tgcactcaat accagttcaa
      361 ctaccagcgc ggaaagcatg gaataaccgc ctggcgaatg ctcgccataa ccgttagaag
      421 caaaaccgct ggctgccgcg tcaaaacctg ttttaccact ggcaattaaa tacagcagcg
      481 ccgccgcaac aataccgccg acaacctggg caattacgta gccaacgact tcttttgccg
      541 ggaaacgtcc gccagcccat aaaccaatag tgaccgccgg gttaaaatga ccaccagaaa
      601 tatgaccaac agcaaaggcc atcgtcagaa cggtcagacc gaatgccagc gccacgccgg
      661 caaaaccaat gcctaattcc gggaagcctg cggccagtac ggcactacca cagccaccaa
      721 aaacaagcca gaaagtacca aaacattcag ctgctaattt tctgaacata tccacctcaa
      781 ttaaaaattg accctgtgaa aaatatggtc gttttatagg gtcgtcgtaa aaaagtgacg
      841 acggaaataa tgcgcggcta ttttaaaaac gaaggcgagt cattcaccag ataaataaat
      901 ccagtaaatt tgatttaagg caacagcggg ttgccccata tagtcatttg tctgattgac
      961 agtgtagtgc aagcgaaaga tttaatcctt taggcgtaat aaaaaataat ttatcatgct
     1021 aattatttga ttttgttgtt tttgcagact tatcagcaag agggagtata acgcgattat
     1081 tcgctcattt ttcagacatt tgccatgctt aaatgtgatg tcatcacgta ttagcatggc
     1141 ctttcccgtt atactgccag cgtaaaggat aagtcacata tttctggagg ggatatgatt
     1201 cttgagcgcg ttgaaattgt gggttttcgc ggtatcaacc gtttgtcgtt gatgctggaa
     1261 caaaacaacg tcctgattgg ggagaacgcg tggggtaaat ccagcttgct ggacgcctta
     1321 accctgctgc tatcgccaga atcagatctc taccattttg agcgcgacga tttctggttc
     1381 ccgccgggag atatcaacgg gcgagaacat catctgcata ttattttgac cttccgcgaa
     1441 tcgctgccag gccgacatcg ggttcgccgc tatcggccgc tggaagcgtg ctggacgcca
     1501 tgcaccgatg gttatcaccg tattttttat cgtctggaag gggagagtgc ggaagacggc
     1561 agcgtgatga cactgcgcag ttttctcgat aaagacggac atccgattga tgttgaggat
     1621 attaacgatc aggctcgtca tctggtgcgt ttaatgccgg tgctgcgctt gcgtgatgcc
     1681 cgttttatgc gccgtattcg taacggcacg gtgccaaatg tccccaatgt ggaagtcacc
     1741 gcgcgccagc tcgatttcct cgcccgtgaa ttatcctcac atccgcaaaa tctctctgat
     1801 gggcagattc gtcagggact ttccgcaatg gtgcaacttc ttgagcatta tttttctgag
     1861 cagggagctg gacaggcgcg atatcgttta atgcggcggc gagccagcaa tgagcaacga
     1921 agctggcgct atctggatat catcaaccgg atgattgacc gacctggagg gcgctcgtat
     1981 cgggttattt tgctcggcct gtttgctact ttattgcagg caaaaggcac attgcgactg
     2041 gataaagacg cccgtccatt gttgctgatc gaagatccag aaacccgttt acaccccatt
     2101 atgctttcag ttgcctggca tctgttgaat cttctgccct tgcagcgcat tgccaccacc
     2161 aactcgggtg agttactttc gttaacgccg gtagagcatg tttgccgact ggtacgtgag
     2221 tcctcgcgcg ttgccgcctg gcgtctgggg ccgagtggct tgagtaccga agatagccga
     2281 cgcatatcct ttcacattcg ttttaaccgt ccgtcatcgc tgtttgctcg ctgctggctg
     2341 ctggtggaag gggaaacgga aacctgggtt atcaatgaac tggcgcgtca gtgcggacat
     2401 cattttgatg ccgaaggaat caaggtcatt gagtttgccc agtccgggct aaagccactg
     2461 gttaaatttg cccgccgaat ggggattgaa tggcatgtac tggtcgatgg cgatgaagca
     2521 gggaagaaat atgccgctac ggtacgcagc ctgttgaata acgatcggga agccgaacga
     2581 gaacatttaa cggcgttacc ggcgctggat atggaacatt ttatgtatcg ccagggattt
     2641 tccgatgtgt tccaccgcgt ggcgcaaatc ccggaaaatg taccgatgaa tctgcgcaaa
     2701 attatctcga aagcgatcca tcgctcttcc aaacccgatc ttgccattga agtggcaatg
     2761 gaggccggac gtcgtggtgt ggactccgta ccgacgctgc tgaaaaaaat gttctcacgc
     2821 gtgctgtggc tggcgcgcgg tcgcgcggat taaccgcgaa acatcgtggc catttgtggc
     2881 tgaatagcgt cgagcatctc atagcgccga cggtattcag cccgtttttt actggcgatt
     2941 tcggcaatct cttttcgtgc tatctgtgcc ggaaggcggt aatggcgttc agcatcacat
     3001 acgccgccaa ccgactccca gaacgcgttg taatcggcat ggatcttgcc ttctttatca
     3061 cgataacgca ggctgcggta aatatgcgtt tcattgctga cggcaataat ctgctctacc
     3121 tgcaaacgtt gggcaaacag acaggccgct tccatcacaa ggcgtttggg aaatagcccg
     3181 tggcaggctt tcgtcgcatt ctggatttcc tgatgtggaa tttcccattt tgcgccttgc
     3241 agtccgccaa taaacattgt tcttttcccc ttatattcac acagggtaaa cgtgatctct
     3301 gccagaggaa taccttcgct gttgcggaac aggattgtgc tgtcaccttc tttatccatt
     3361 gagatcatca tggtcagctc aagcgtgaac tgctcgccgt ttttgccttc cagtttagcc
     3421 agttgcagcc cgggggtatt caaatataag ctgaattctt ccgccgacat acacccgcgg
     3481 agtaacgcat aatggtaacg taacgcctcc agcaattgct tacggctaag attcgccgca
     3541 aggtaagggc gatgcagacg cacaggcagt cgcggctggc gcgttaacaa cacattgaga
     3601 ttgggccagt gggaaagttc gttcatccac tcaacgctta aacgcggcat aatcaacgag
     3661 cgcagcaaaa atttctggcg aaaactacgg cgatgccaga atttacccgg ccgacactgt
     3721 ccacgtgcca gactaagaaa aagtgacagg ctgctgagag attcagatgg cgtaaaggtc
     3781 cgttcagtta gctgcgacat attcatgaaa tcaatggtta tacatgacgt cgatttcacc
     3841 attgcgtatc ttaaccaaac atcaatagtg tgattactaa tgtaaatttt agggttttgt
     3901 tgatatttcg ttgaagttaa tgacccggat tggcatatgg agtattcaga atatttatga
     3961 aaaagcggaa aaccgtgaag aagcgttacg ttattgcgct ggtgataatc atcgtcgggc
     4021 tgattacgtt atggagaatt cttaacgcac ccgtgccgac ttatcagacg ctgattgtgc
     4081 gccccggtga tttacagcaa agcgtgctgg cgaccggaaa gctggacgcg ctgcgtaagg
     4141 ttgatgtggg cgcgcaggtc agcggtcagt tgaaaacgct gtcggtggcg attggcgata
     4201 aagtaaaaaa agaccagctt ttaggggtta ttgatcctga acaggctgaa aaccagatta
     4261 aggaggtgga agcaacgctg atggagctac gcgcgcagcg gcagcaggcg gaagcggagc
     4321 tgaaactggc gcgggtgacg tattcccgtc agcaacgtct tgcacaaacg caagcggttt
     4381 cacagcagga tctcgataac gccgcgacgg agatggctgt gaaacaggcg caaattggca
     4441 ccattgacgc gcaaatcaag cgcaatcagg cctctctcga tacggctaaa accaatctcg
     4501 attacacccg catcgttgcc ccgatggccg gggaagtcac gcaaatcacc actctgcaag
     4561 gccagacggt gattgccgca caacaagcac cgaacattct gacgctggca gatatgagca
     4621 ccatgctggt aaaagcgcag gtttctgaag cggatgtaat ccacctgaag ccggggcaaa
     4681 aagcctggtt tacggtgctt ggcgatccac tgacgcgcta cgaggggcaa atcaaggatg
     4741 tgctaccgac gccggaaaag attaacgacg ctattttcta ttacgcccgt tttgaagtac
     4801 ccaaccccaa tggtttactg cggctggata tgaccgcgca agtgcatatt cagctcaccg
     4861 atgtgaaaaa tgtgctgacg atccctctgt cggcgttagg cgatccggtt ggcgataatc
     4921 gttataaagt caaattgttg cgtaatggtg aaacacgcga gcgtgaagtg acgattggcg
     4981 cacgtaacga taccgatgtt gagattgtca aagggctgga agcgggcgat gaagtggtga
     5041 ttggtgaggc caaaccagga gctgcgcaat gacacccttg ctcgaattaa aggatattcg
     5101 tcgcagctat cctgccggtg atgagcaggt tgaggtgctg aagggcatca gcctcgatat
     5161 ttatgctggt gagatggtgg cgattgtcgg cgcttcgggt tccggtaaat cgaccctgat
     5221 gaatattctc ggctgcctgg ataaggccac cagcggcacc tatcgcgtag ccggtcagga
     5281 tgttgccacg ctggacgccg atgcgctggc gcaactacgc cgcgagcatt tcggctttat
     5341 tttccagcgt taccatttgc tttcgcattt aaccgccgag cagaacgttg aagtacccgc
     5401 cgtctatgcc ggtcttgagc ggaaacagcg gctgcttcgc gcccaggagt tgctgcaacg
     5461 actggggctg gaagaccgta cagagtatta cccggcacag ctttcgggtg gtcagcaaca
     5521 gcgcgtcagc atagcgcggg cattgatgaa cggcggtcag gtaattcttg ccgatgaacc
     5581 gaccggcgca ctggacagcc attctggcga agaggtgatg gcgatcctgc atcagctgcg
     5641 cgatcgcggg catacggtga ttatcgttac ccacgatccg caggtcgctg ctcaggccga
     5701 gcgggtgatc gaaattcgcg acggtgaaat tgtgcgcaat cctcccgcca ttgaaaaagt
     5761 gaatgttgct ggcgggacgg aacctgttgt caacacggcg tctggctggc ggcagtttgt
     5821 cagcggtttt aacgaggcgc tgacgatggc atggcgggcg ctggcagcga ataaaatgcg
     5881 tactttactg accatgctgg ggattattat cggtattgcg tcggtggttt ccattgtcgt
     5941 ggtgggtgac gccgccaaac aaatggtgct ggcggatatt cgttctattg gtacgaatac
     6001 tattgatgtc tatcccggga aagattttgg cgatgacgat ccgcaatatc agcaggcgct
     6061 gaagtacgac gacttaatcg ccatccaaaa acaaccgtgg gtcgcctcag ccacacctgc
     6121 cgtctcgcaa aacctgcgcc tgcgttataa caatgttgat gtggctgcca gtgccaatgg
     6181 cgtgagcggc gattatttta atgtctatgg catgaccttc agtgaaggga acacctttaa
     6241 tcaggagcag ctgaacggtc gtgcgcaggt ggtggtgctc gacagtaata ctcgccgcca
     6301 gcttttcccc cataaagcag atgtggttgg cgaggtgatt ctggtcggca atatgcccgc
     6361 cagagtcatt ggtgtggcgg aagaaaaaca gtcgatgttt ggtagcagta aagtgctgcg
     6421 tgtctggcta ccttacagca cgatgtccgg gcgagttatg ggccagtcgt ggctgaactc
     6481 cattactgtc agggtgaaag aaggctttga cagcgctgag gcggaacagc aactcacgcg
     6541 tttactttca ctgcgccacg gtaagaagga tttctttacc tggaacatgg acggcgtctt
     6601 gaaaaccgtt gaaaagacca cacgtacttt acaactgttt ctgacgctgg tggcggtgat
     6661 ttcgctggtg gtgggcggta ttggtgtaat gaatattatg ctggtgtcag tgaccgagcg
     6721 gacgcgggaa attggcattc gcatggctgt aggtgcgcga gcaagcgatg ttttgcaaca
     6781 gttcctgatt gaagccgtac tggtttgcct ggtcggtggc gcgttgggaa taacactgtc
     6841 actgttaatt gctttcacct tacagctttt cttacccggc tgggagattg gtttttcacc
     6901 gttggcgctg ctgctggcgt ttctctgctc gacggtcacc gggattttat ttggctggtt
     6961 acccgcacga aatgcggcac gactggatcc agtagatgct ctggcacgag agtaattttt
     7021 gagataaaaa tgccagccga tcgggctggc attttgcctt taggatgtac acaatgagac
     7081 agaagagcta tgcgactgcc gcttctactt cgacgggcac aataacactg gcgtgattgc
     7141 cttttggccc ctggtggaca tcaaactgaa cggattgtcc agcttttagc gttctgtaac
     7201 catccatctg aatggtggaa taatgagcga aaatatcttc gccgccgcct tcagggcaga
     7261 tgaaaccaaa ccctttggca ttgttgaacc acttaacagt acccttttcc atgcttcgac
     7321 atccttcgca aatcttatac aagtaagatg gaataaaccg gggtcagaga gggggctgtt
     7381 caaaacctcg ccaactctag aaatacaatt tagagaatta gggcgagccg tcaagcattt
     7441 gacaggggcc agggggcagg tatgaatcaa aaatttgaag cagttaacgc tattgacagg
     7501 aatgtgacag atgtcgctga tgccaacgat agatgatagt tatctatcat gtggagtaga
     7561 ttggtcaggc aaataagctc ttgtcagcgg cagggcgttc tgccgataac cgtaaccgaa
     7621 gatgataact gacaatgggt aaaacgaacg actggctgga ctttgatcaa ctggcggaag
     7681 aaaaagttcg cgacgcgcta aaaccgccat ctatgtataa agtgatatta gtcaatgatg
     7741 attatactcc gatggagttt gttattgacg tgttacaaaa attcttttct tatgatgtag
     7801 aacgtgcaac gcaattgatg ctcgctgttc actaccaggg gaaggccatt tgtggagtct
     7861 ttaccgctga ggttgcagaa accaaagtgg cgatggtgaa caagtacgcg agggagaatg
     7921 agcatccatt gctgtgtacg ctagaaaaag cctgaatgca ggcataaaaa ttgggggagg
     7981 tgcctatgct caatcaagaa ctggaactca gtttaaatat ggctttcgcc agagcgcgcg
     8041 agcaccgtca tgagtttatg accgtcgagc acttgttact ggcgctgctc agtaacccat
     8101 ctgcccggga ggcgctggaa gcgtgctctg tggatttggt tgcgctccgt caggaactgg
     8161 aagcctttat tgaacaaacc acacccgttc tgcctgccag tgaagaggag cgcgacacac
     8221 agccgacgct gagttttcag cgtgtactgc aacgtgcggt cttccatgtc cagtcctccg
     8281 gtcgcaatga ggttaccggt gcaaacgttc tggtcgctat ctttagcgaa caggagtcgc
     8341 aggcggcata tctgttgcgc aaacatgaag tcagccgtct cgatgtggtg aactttatct
     8401 ctcatggcac gcgtaaagac gagccgacac agtcttctga tcctggcagc cagccaaaca
     8461 gcgaagaaca agctggtggg gaggaacgta tggagaattt cacgacgaac ctgaatcagc
     8521 ttgcgcgagt gggcggaatc gacccactga ttggtcgtga gaaggagctg gagcgtgcta
     8581 ttcaggttct ctgccgtcgc cgtaaaaaca acccgctgct ggtgggggaa tctggtgtcg
     8641 gtaaaaccgc gattgcggag gggcttgcat ggcgaattgt tcagggcgat gtgccggaag
     8701 tgatggctga ctgtacgatt tactctctcg atatcggttc tctgttagcg ggcacaaaat
     8761 atcgcggcga ctttgaaaaa cgttttaaag cgttgctcaa gcagctggag caagacacta
     8821 acagcatcct gtttattgat gagatccaca ccattatcgg tgcgggtgca gcgtctggtg
     8881 gccaggtcga tgcagctaac ctgatcaaac cgttgctctc cagcggtaaa attcgcgtaa
     8941 ttggttcgac aacctatcag gagttcagca acattttcga gaaagaccgt gctctggcgc
     9001 gtcgcttcca gaaaattgat attactgaac cgtcaatcga agaaactgtt caaatcatca
     9061 atggcctgaa accgaagtat gaagcgcacc acgacgtgcg ttataccgca aaagcggtgc
     9121 gtgcagcggt agagctggcg gtgaaataca ttaacgatcg tcatctgccg gataaagcca
     9181 ttgacgttat cgacgaagcg ggcgctcgcg cacgcctgat gccggtaagc aaacgcaaga
     9241 aaaccgttaa tgtggcggat attgagtccg tggtggcccg tattgcgcgc attccagaga
     9301 agagtgtttc gcagagtgac cgcgataccc tgaaaaacct cggcgatcgc ctgaaaatgc
     9361 tggtcttcgg tcaggataaa gccattgagg cgctgactga agccattaag atggcgcgtg
     9421 caggtttagg tcacgaacat aaaccggtcg gttcgttcct gtttgccggt cctaccgggg
     9481 tcgggaaaac agaggtgacg gtacagcttt cgaaagcgtt gggcattgag cttctgcgct
     9541 ttgatatgtc cgagtatatg gaacgccata ccgtcagccg tctgattggt gcgcctccgg
     9601 gatacgttgg ttttgatcag ggcggtttgc tgactgatgc ggtcatcaag catccacatg
     9661 cggtgctgct gctggacgaa atcgagaaag cgcacccgga cgtgttcaat attctgttgc
     9721 aggtgatgga caacggtacg ctgaccgata acaacggacg caaagcggac ttccgtaacg
     9781 tggtattggt gatgaccacc aacgctgggg tacgagaaac tgagcgtaaa tcgattggtc
     9841 ttatccacca ggacaacagt accgatgcga tggaagagat caagaagatc tttacgccgg
     9901 agtttcgtaa ccgtctcgac aacattatct ggttcgatca tctctccacc gacgtgatcc
     9961 atcaggtagt ggataaattc atcgtcgagt tgcaggttca gctggatcag aaaggtgttt
    10021 ctctggaagt gagccaggaa gcgcgtaact ggctggccga gaaaggttac gaccgggcaa
    10081 tgggcgcacg tccgatggcg cgtgtcatcc aggacaacct gaaaaaaccg ctcgccaacg
    10141 aactgttgtt tggttcgctg gtggacggcg gtcaggtgac ggttgcgctg gataaagaga
    10201 aaaatgagct gacttatgga ttccagagtg cacaaaagca caaggcggaa gcagcgcatt
    10261 aatctaattg ttaggtaggt tggtgaagtc cgtaatctcg aaagaggtta cggacttttt
    10321 gtttatgggg tggaggaggt tctgaccctt tttttgatga tgatggtaat ttgttgataa
    10381 ttagtgctgc gggaaggtaa ggataaaaaa gggtggtagc aggaaaatga gatggttttg
    10441 ctttattaac aacgggctag acgtgtagta tttgagttca ctgccgtaca ggcagcttag
    10501 aaagccaacc gaaagaaaaa ctcgattttt ataccgttca ctgccgtaca gacagataaa
    10561 atgcgaaaaa aaaaacgtac tttcgtacga gctcttcttt gaatatggcg gtgagggggg
    10621 gattgactcg cttcgctcgc cctgcgggca gcccactcac tgtgttcgtg gtctgtccaa
    10681 ctggctgcgc cagttgtcga accccggtcg gggcttctca tcccccctgg agtgcaatat
    10741 gcgaaaaaaa agctcgcact ttcgtacgag ctcttctttg aatatggcgg tgaggggggg
    10801 attcgaaccc ccgatacgtt gccgtataca cactttccag gcgtgctcct tcagccactc
    10861 ggacacctca ccaaattgtt ttgctaccaa acctcatggg tggcaacggg gcgctactat
    10921 agggagttgg agtaaaacgg tcaagaagaa ttttaatgat aattattgtt tgctcatact
    10981 gtaaacaagt tgtgcagtat atctacatcg agacaagtta tggacttata cttccaaagt
    11041 acttcataca tatcacaaaa taaaaaggcc ggctaaaccg accttttact cgttctttct
    11101 cttcgcccat caggcggtaa aacaatcagc gactacggaa gacaatgcgg cctttgctca
    11161 ggtcgtacgg ggtcagttca acagtcactt tgtcgcccgt caggatgcgg atgtagtttt
    11221 tgcgcatttt accggagatg tgtgcagtaa ccacgtgacc gttttctaac tctacgcgga
    11281 acatggtatt aggcaacgtt tcaagaacgg taccttgcat ttcaatattg tcttctttgg
    11341 ccatctaatc ctctggggta tcactaccgt aatttgaacc ggcaagataa tgccgaagtt
    11401 ctgtaaataa gtaaagattt gcgcgctaaa tcgcaacaaa caggttcggc acattactcc
    11461 gaaaacacac ggctaagccg caccaaaagc gcaacgtata agggagcggt gagataaacg
    11521 atgggcgtta cctgacgcga aaaattcctt atcggcagcg gggtaatgag cgtaaccaac
    11581 tctgcgaccg caattataac actctgggga gaaatgtgcc gaaaacattc attcttgtgg
    11641 tgaaaacaag caccgtggta cccagaaatt attcggcaat cgtccgaggc gcatttgatt
    11701 gagataattg aggtaatccc ggcggggaat ttcgcaggca ccaagcgacg ctgtgtgatc
    11761 gttaaggacc tggcagtcga taagtttacc gccatgacca ataaattcct cacagaatac
    11821 cagaagcgcc gttttagacg cattttccat ccggctgaac atggactcgc cacaaaatag
    11881 cgttccctgg gccacgccgt acataccgcc gacaagctca tcttcacgcc agacttcaat
    11941 agagtgggca tgaccgagtt cgtgaaggcg atggtaggct tcgaccacgc cacgcgtgat
    12001 ccaggttcct tcttcgcgat cgctggcaca gccttcaatg acctgaccaa aagcgtaatt
    12061 catcgtgact cgatagggcg agcgtttatg aaatcgcttc atactacggc tgatatgcag
    12121 tgattcaggc caaagcaccg cgcggggatc gggcgaccac cagaggatgg ggtcacctgg
    12181 agaaaaccac ggaaaaatac cacgctggta agccattaac aggcgcgcag ggctaagatc
    12241 gcctcccagt gccagcaggc cgttaggctc acgtaatgcg ccttccgggg aagggaaggc
    12301 tattgaatgg cgagaaagct gaaccaggcg catgaccgca aaactccacg caagtcggat
    12361 cgttcaataa tagcttacaa accctgctta aactggtaat aacgcccctg tctggcaagc
    12421 agttctgcgt gagtaccttg ctcaataatt tgcccgttgt ccatcactat tatttgttgg
    12481 aaacgagaga gtccgcgaag tcgatgggtg accattaaca ccgttttctc atgcatcatt
    12541 tctgcaagca attcaaggat ctgactttcg gttgtggcat ctaagccttc tgtaggttca
    12601 tccagcaata ccagcggcgc attatgtaac agcgcacggg cgatagccag acggcgtagt
    12661 tcaccaccgg agagctggcg tccgccttca cccaaccaac tgttgagacc tgcatcctcg
    12721 agcagcttct ccagaccaac gcgacgtaag atctccgcca gagcctcatc actactgcca
    12781 ggcgaggcga gtaacagatt atcacgcagc gtggcgctaa acagatgcac tcgctgagga
    12841 acaacgctga tggtctgtcg tagagtcgct tcattcaggc tggctatggg gctaccgtta
    12901 agcaaaatct cgccctgttg cgggtcccat gcgcgggtca gcagttgtaa cagtgttgat
    12961 ttgccgcatc cggttcgccc gagaatcgct atatgttccc cggcgtttac ctgaagagaa
    13021 atccctttaa gtgcctgttg ggattgctcc ggataattga attgaacatc ccgtaacgtc
    13081 agcgaaacgc gatcggcaac acgagtttgg gtatcaggga aggtgacttc cggtttttga
    13141 tccgttaagt cagtgatccg tacggcagag gcaatgactt gccccagatg ctgaaatgca
    13201 cccgttactg gtgccagtgc ttcaagcgcg gctaacgcgc agaagacaaa cagggcaatt
    13261 aacgcgccgg gttgagcatt gccgccaacg ccgccagacg ccatccacag catcaggatc
    13321 accgctaacg cgccaatgag cagcattatc gcttgcgaca atgcggtgag ttcagattga
    13381 cgacgttgcg cttccagcca ctgaatttct gtattctcta attgcgtgcg ataacgatcg
    13441 ctggcaccaa agatggtcag ctcagcttgc ccttgcagcc aggccgtcag ttgttggcga
    13501 tactgcccgc gaagatgagt cagattttgc ccggtgcttt ttcccgcacg ataaaacagt
    13561 ggtggcataa ggaaaagcgt cagtaacata atgccgccca gcgtaaaggc gagggtgaaa
    13621 tcaaggaaac ttaacccgat tgtcaccacc ataatcacca caaaagcgcc caccagcggc
    13681 gagataacgc gcaggtaaag atgatcgagc gtatcaacat ccgccaccac gcggttgagc
    13741 aattcgccct gacgatagcg cgccagtccg gcaggggata ggggcagcaa tttgctgaag
    13801 gtgtaaatgc gcagatgctg caacacgcgg aaagtcgcgt cgtgacttac cagacgttca
    13861 aaatagcgcc cggcagtacg ggtgattgct gcgccacgca cgcccgcagc gggtagcata
    13921 tagttgaagc tgtacagtcc ggcaaccccc gctaccgctg aggcggagag gaaccagccg
    13981 gaaagtgtca acagaccgat actggcgagc agcgtgacaa ttgccagcac aataccaaga
    14041 cttaacatcc atttatgacg tttatacagt gccagatagg gtagcaaagc gcgcatttaa
    14101 atctcctcct gacgatgggc cagcaatgtg gcgaaaggac caccagccac gcttaattcc
    14161 gcgtaacgtc cttgctcaat aatctgacca tcctgcatta cccaaatgac atcccagtca
    14221 gcaagatctt ctaactggtg ggtgaccatt aacgttgtct ggcgcagaga ggcggcatta
    14281 agcgcctcca ttacgcgctg ttcactgtga gcatcaaggc tggcagcggg ttcatccaac
    14341 agtaatagcg aacagggatt tagtaatgct cgggccaccg ccacgcgctg cgcctgcccc
    14401 acggaaaggc gggcagcctg gtcgccaaca ggcgtatcaa cgccttgtgg caggagcggt
    14461 agaaactcgc tgacccaggc gttatccagc gctgtttgta actcgtgttc gctggcatca
    14521 ggtcgtgcca gtagtacgtt atcccgtagt gttgctgccg gtaattgtgg gttttgccca
    14581 acccaggaga gatgcttacg ccatgattcc ggtgataaat cgcgtaattc tatcccgttg
    14641 attcgtagcg atccctgata tgagagaaaa ccagaaagcg cgttcagcag tgagctttta
    14701 cctgaaccgc tgcgaccaac caacactgca cgttggcctg ctggcaaagt aaagttcagc
    14761 ggtccagcca gcgttttacc ttctggtgat gtaataaaca gatcctcggc ttcaatggtc
    14821 accggatcgg ttgatgctaa ttccgcctca ccacgttgcg gatgggctag cggggtttcc
    14881 ataaacgttt tcagactgtc agctgcgcca acagcctggg ctttagcatg ataaaacgta
    14941 ccgagatcgc gtaatggctg gaaaaactcc ggcgcaagga tcagggccag aaaacccgca
    15001 gccagcgtca cgccagtatc atagtgacca aaatccagct cgccgagata ggaaaaacca
    15061 aagtagaccg ccaccagagc aattgacagc gaggtaaaaa attcgagaat gccggaggat
    15121 aaaaacgcca gccgtagcac ttccattgtc cgttggcgga aatcttccga agcagaacga
    15181 atactttcaa tttcagcttc accacgacca aaaatacgca atgtttccat gccgcgcagg
    15241 cgatcgagga aatgcccact taagcgagca agagcgagaa agttacgtcg gttagcatcg
    15301 gcagccccca ttccaaccag cgccataaac aacggaatta acggtgcagt gcccagcaga
    15361 ataagcgccg cagcccagtt agacgggaag atcgccacca caatcagcaa aggcacggac
    15421 actgccagcg ccatttgcgg caggtagcgt gcatagtaat catgcatatc gtcaatttgc
    15481 tcgagtacca gcgtcgccca gctccccgcc ggtttaccct gaatccacgc tggccctgct
    15541 tgttgcagac ggtcgagaac ctgacggcgg atggcaaagc ggatatgctg cccggcgtga
    15601 taacccaccc gttcgcgtaa ccagaccacc catgcgcgca gtacaaaggt cagaaccagt
    15661 aacgtaaagg gaagcagcag ggcttcacgg ggaatattct ccataatcat atgttgcaga
    15721 attcgcgcca tgaaccaggc ctgggcaatg atcaatatgc cgctcacaaa gcccagcaga
    15781 cgagaaatat tcagccaacg ttgggagatg acgctttgct gttttaacca gcgggttaac
    15841 tctttttgac gggatttatt cattgcacgc ttagcaggtg agttatcaga attatttgca
    15901 gagcaatgtt acaacgagga aaaaataaag gcgacccata gtcgcatggt gtcgccttct
    15961 ttacttttgt tactgatttg taaaattatt ttgcgtcagc taaaccatcg aggtagcgtt
    16021 ccgcatcaag tgctgccatg cagcctgtac cagcagatgt aatagcctgg cgataaatgt
    16081 gatccatcac gtcgcctgcg gcaaagacgc cagggatgct ggtctgggtg gcattaccat
    16141 gaatacccga ctgtactttg atgtagccgt tttccagttc cagctgccct tcgaaaatag
    16201 ccgtattcgg gctgtgaccg atagcaacaa acagaccggc aacgtcgagt gactcgatgt
    16261 tatcgctgtt ttgcgtatcg cgcagacgaa cgccagtgac gcccatctga tcgccggtca
    16321 cctcttccag cgtacggttg gtgtgtagaa tgatgttgcc gttctccact ttatccatca
    16381 gacgcttaat aaggattttt tccgcgcgga aaccgtcacg gcggtgaatc agatgcactt
    16441 ccgaagcgat gttagacaga tacagcgcct cttcaaccgc ggtattgccg ccgccgatga
    16501 ccgcaacttt ctggttgcga tagaagaaac cgtcgcaggt agcacaagca gaaaccccac
    16561 ggcctttaaa ggcttcttca gatggcaggc ctagatagcg tgcagaagct ccggtggcaa
    16621 taatcagcgc gtcgcaagtg tattcgccgc tatcgccagt cagacggaac ggacgattct
    16681 gcaaatcaac tttgttgatg tgatcaaaga tgatttcagt ttcaaatttg gtggcatgtt
    16741 cgtgcatgcg ctccattaat aacggaccgg tcagatcgtt tggatcgcca ggccagtttt
    16801 ccacttccgt ggtggtggtc agttggccgc ctttttccat gccggtaatc agcacaggtt
    16861 gcaggttggc gcgcgccgcg tagacagcag cggtatatcc cgccgggcct gaacccagga
    16921 taagcagttt actgtgtttg gtcgtgccca tgagatcccc atagttgttg gcagacaatg
    16981 ggcaggattg tagggaattt acagacgtaa aaaaagagta tgacgatttt gttaacaatt
    17041 tgtgcaatcg gcagcatcga taagcaggtc aaattctccc gtcattatca cctctgctac
    17101 ttaaatttcc cgctttataa gccgattaaa tgatgaataa acgcccctgt taatgaatat
    17161 ctggcatgtt gtactaaaaa tcgatgtttt gctttgacaa tcccctggtg ttttgcgaaa
    17221 acattcgagg aagaaaaaaa cagtattctt atatgcgcat aaccatgcat gtaaatacca
    17281 tgtttaccgt gctagtgaaa tctacgtatg gcgtggacag acgccattcg tgatgtcgat
    17341 agctgccaca aggcaacggt cttctcaccg tagacccagg cattgcgcgc cgtgaatctt
    17401 catgatttcg gtctatcgtg acgggtagcg actctgaaca gtgatgtttc agggtcagac
    17461 aggagtaggg aaggaataca gagagacaat aataatggta gatagcaaga agcgccctgg
    17521 caaagatctc gaccgtatcg atcgtaacat tcttaatgag ttgcaaaagg atgggcgtat
    17581 ttctaacgtc gagctttcta aacgtgtggg actttcccca acgccgtgcc ttgagcgtgt
    17641 gcgtcggctg gaaagacaag ggtttattca gggctatacg gcgctgctga acccccatta
    17701 tctggatgca tcacttctgg tattcgttga gattactctg aatcgtggcg caccggatgt
    17761 gtttgaacaa ttcaataccg ctgtacaaaa acttgaagaa attcaggagt gtcatttagt
    17821 atccggtgat ttcgactacc tgttgaaaac acgcgtgccg gatatgtcag cttaccgtaa
    17881 gttactgggg gaaaccctgc tgcgtctgcc tggcgtcaat gacacacgga catacgttgt
    17941 tatggaagaa gtcaagcaga gtaatcgtct ggttattaag acgcgctaac acggaacagg
    18001 tgcaaaatcg gcgtattttg attacactcc tgttaatcca tacagcaaca gtactggggt
    18061 aacctggtac tgttgtccgt tttagcatcg ggcaggaaaa gcctgtaacc tggagagcct
    18121 ttcttgagcc aggaatacac tgaagacaaa gaagtcacat tgacaaagtt aagtagcggc
    18181 cgccgccttc tggaggcgtt gctgatcctt attgtcctgt ttgccgtctg gttgatggct
    18241 gccttactaa gctttaaccc ttcggacccc agctggtcgc aaacggcctg gcatgaacct
    18301 atccataatt taggtgggat gcccggtgcg tggctggcag atacgctgtt ctttattttt
    18361 ggcgtgatgg cttacaccat tcccgtcatt attgtcggcg gttgttggtt tgcctggcgt
    18421 catcagtcca gcgacgaata cattgattat tttgccgttt cgctacgcat cattggcgtt
    18481 ttggcgctca tccttacctc ctgtggtctg gcggcaatca acgctgacga tatctggtat
    18541 tttgcctccg gtggcgtcat tggcagctta ctaagcacta cgctacaacc actgctacac
    18601 agtagcgggg gaactattgc gctgctctgc gtttgggctg cgggtctgac gctgttcacc
    18661 ggttggtcat gggtgaccat tgctgaaaaa ctcggcggct ggattttaaa cattctcacc
    18721 ttcgccagta atcgtacccg tcgcgatgat acctgggtcg atgaagatga gtatgaagac
    18781 gacgaagaat atgaagatga aaatcacggt aaacagcatg aatcacgccg tgcccgtatt
    18841 cttcgcggcg cgttagcgcg tcgtaaacgg ttggcggaaa aattcattaa tccgatgggg
    18901 cggcaaacag atgctgcgtt gttctccggt aagcggatgg atgatgaaga agagattacc
    18961 tacactgcac gcggtgtggc tgccgaccct gacgacgtcc tcttttcggg caatcgtgca
    19021 acgcagccag aatatgacga atacgatcca ttattaaacg gtgcgccaat taccgaacct
    19081 gttgctgtag cagctgctgc taccacggcg acacaaagct gggctgcgcc ggttgaacct
    19141 gtgactcaga cgccgcctgt tgcctctgtt gatgttccac cttcgcaacc tacagtagcc
    19201 tggcagcctg taccgggtcc acaaacggga gagccggtga ttgctccggc accggaaggt
    19261 tacccacagc agccacaata tgcgcagcct gcagtgcaat ataatgagcc gctgcaacaa
    19321 cctgtacagc cacagcagcc gtattatgcg ccagcagcgg aacaacctgt gcaacaacct
    19381 tactacgctc ctgcggcgga acaacctgtt cagcagccgt attatgcaac tgcggcggaa
    19441 cagtctgcgc aacagccgta ttacgcccct gcgccagaac aaacggcggc aggtaacgcc
    19501 tggcaagccg aagagcagca atccactttt gctccacagt ctacatacca gactgagcaa
    19561 acttatcagc agccagccgc tcaggagccg ttgtaccaac agccgcaacc cgttgaacag
    19621 caccctgttg tggagcctga gcccgttgta gaagagacaa aacccgcgcg tccgccgctt
    19681 tactattttg aagaagtgga agagaagcgt gcccgtgaac gtgaacaact tgcggcctgg
    19741 tatcaaccga ttcctgaacc ggttaaagaa ccagaaccga tcaaatcttc gctgaaagca
    19801 ccttctgttg cagcagtacc tccagtggaa gccgctgccg ctgtttctcc gctggcatct
    19861 ggcgtgaaaa aagcgacact ggcgacgggg gctgccgcaa ccgttgccgc gccagtcttc
    19921 agtctggcaa atagcgctgg accgcgtccg caggtcaaag aggggattgg cccgcagttg
    19981 ccacgaccga aacgtatccg cgtgccaact cgtcgtgaac tggcgtctta cggtattaag
    20041 ctgccctcac agcgtgcggc agaagaaaaa gcccgtgaag ctcagcgcaa tcagtacgat
    20101 tccggcgatc agtataacga tgatgaaatc gatgcgatgc agcaggatga actggcacgt
    20161 cagttcgccc agacacagca gcaacgctat ggcgaacagt atcaacatga tgtgcccgtg
    20221 aacgcagaag atgcagatgc tgcggcagag gctgaactgg ctcgtcagtt tgctcaaact
    20281 caacaacaac gttattccgg cgaacaacct gctggggcga atccgttcac tctggatgat
    20341 tttgaatttt cgccaatgaa agcgttgctg gatgatggtc cacacgaacc gctgtttacg
    20401 ccaattgttg aacctgtaca gcagccgcaa caaccgattg caccgcagca acagtatcag
    20461 cagccgcagc aaccagttgc gccgcagccg cagtatcagc agccgcaaca gccggttgca
    20521 ccgcagcagc aatatcagca gccacaacaa ccggttgcac cgcagccgca atatcagcag
    20581 ccgcaacagc cggttgcacc acagccgcaa tatcagcagc cgcaacagcc agttgcgccg
    20641 cagccgcatg ataccctgct tcatccgctg ttgatgcgca atggtgacag ccgtccgttg
    20701 cataaaccga cgacgccgct gccttctctg gatttgctga cgccgccgcc gagcgaagtg
    20761 gagccggtag atacctttgc gcttgaacaa atggcgcgcc tggtggaagc gcgtctggct
    20821 gatttccgta ttaaagccga tgtcgtcaat tattctccgg ggccggttat cactcgcttt
    20881 gaattgaacc tggcaccggg agtaaaagcg gcgcgtattt ctaacttgtc acgtgacctt
    20941 gcccgttcac tttcgacggt ggcggtgcgt gtcgttgaag ttattcctgg caaaccctat
    21001 gtagggctgg agttaccgaa taaaaaacga caaaccgttt atctgcgcga agttttggat
    21061 aacgccaaat tccgcgataa tccgtcgcca ttaaccgtgg tgctgggtaa agatatcgcc
    21121 ggtgagccgg tggttgccga tctggcgaaa atgccgcact tgttggttgc ggggactacc
    21181 ggttccggta agtctgtcgg tgtgaacgcg atgatcctga gcatgcttta taaagcacag
    21241 ccagaagatg tgcgtttcat catgatcgac ccgaaaatgc tggagctttc ggtttatgaa
    21301 ggcatcccgc atctgttaac ggaagtcgtt actgatatga aagatgccgc caacgcgctg
    21361 cgctggtgtg ttaacgagat ggagcgtcgt tataaactga tgtctgcgct gggtgtgcgt
    21421 aatctggcgg gttataacga aaaaattgct gaagccgatc gcatgatgcg tccgattcca
    21481 gacccgtact ggaagccggg tgatagtatg gatgcgcatc atccggtgct gaaaaaagaa
    21541 ccgtacatag tggtgttggt ggacgaattt gccgacctga tgatgacggt aggtaaaaaa
    21601 gtggaagagc tgatagcacg tctggcgcaa aaagcccgtg ccgcgggtat ccacctcgta
    21661 ctggcaacgc agcgtccatc ggttgatgtt attactggtc tgattaaagc gaatattccg
    21721 acccgtatcg cctttaccgt atccagtaag attgactcac gtaccattct tgatcaggct
    21781 ggcgcggaat cactgctggg tatgggggat atgctctact ctgggccgaa ctccacgttg
    21841 ccggtacgtg tccatggtgc ttttgtacgc gatcaggaag ttcatgccgt ggtgcaggac
    21901 tggaaagcgc gtggtcgccc acagtatgtt gacggcatca cctccgacag cgaaagcgaa
    21961 ggtggtgcgg gtggtttcga tggcgctgaa gaactggatc cgttgttcga tcaggcggtg
    22021 cagtttgtca ctgaaaaacg caaagcgtca atttcaggcg tacagcgtca gttccgcatt
    22081 ggttataacc gtgcagcgcg tattatcgaa cagatggaag cgcaagggat tgtcagcgaa
    22141 caggggcaca acggtaatcg tgaagtgctg gccccaccgc cgtttgacta attaatgcat
    22201 cgtaggccgg ataaggcgcg gtagcgtcgc atccggcact ctaacaactg aaaattcagt
    22261 attttcttct ttcctcaagc tgattattag cctggaatag agagtagagg gaactcccga
    22321 tcgggagtga cgtaatttga ggaataatga tgaaaaaaat tgccatcacc tgtgcattac
    22381 tctcaagctt agtagcaagc agcgtttggg ctgatgccgc aagcgatctt aaaagccgcc
    22441 tggataaagt cagcaacttc cacgccagct tcacacaaaa agtgactgac ggtagcggcg
    22501 cggcggtgca ggaaggccag ggcgatctgt gggtgaaacg tccaaattta ttcaactggc
    22561 atatgactca acctgatgaa agcattctgg tttctgacgg taaaacactg tggttctata
    22621 acccgttcgt tgagcaagct acggcaacct ggctgaaaga tgccaccggt aatacgccgt
    22681 ttatgctgat tgcccgcaac cagtccagcg actggcagca gtacaatatc aaacagaatg
    22741 gcgatgactt tgtcctgacg ccgaaagcca gcaatggcaa tctgaagcag ttcaccatta
    22801 acgtgggacg tgatggcaca atccatcagt ttagcgcggt ggagcaggac gatcagcgca
    22861 gcagttatca gctgaaatcc cagcaaaatg gggctgtgga tgcagcgaaa tttaccttca
    22921 ccccgccgca aggcgtcacg gtagatgatc aacgtaagta gaggcacctg agtgagcaat
    22981 ctgtcgctcg atttttcgga taatactttt caacctctgg ccgcgcgtat gcggccagaa
    23041 aatttagcac agtatatcgg ccagcaacat ttgctggctg cggggaagcc gttgccgcgc
    23101 gctatcgaag ccgggcattt gcattctatg atcctctggg ggccaccggg taccggcaaa
    23161 acaaccctcg ctgaagtgat tgcccgctat gcgaacgctg atgtggaacg tatttctgct
    23221 gtcacctctg gcgtgaaaga gattcgcgag gcgattgagc gcgctcggca aaaccgcaat
    23281 gcaggtcgcc gcactattct ttttgttgac gaagttcacc gtttcaacaa aagccagcag
    23341 gatgcatttc tgccacatat tgaagacggc accatcactt ttattggcgc aaccactgaa
    23401 aacccgtcgt ttgaacttaa ctcggcactg ctttcccgtg cccgtgtcta tctgctgaaa
    23461 tccctgagta cagaggatat tgagcaggtg cttactcagg cgatggaaga caaaacccgt
    23521 ggctatggtg gtcaggatat tgttctgcca gatgaaacac gacgtgccat tgctgaactg
    23581 gtgaatggcg acgcgcgccg ggcgttaaat acgctggaaa tgatggcgga tatggccgaa
    23641 gtcgatgata gcggtaagcg ggtcctgaag cctgaattac tgaccgaaat cgccggtgaa
    23701 cgtagcgccc gctttgataa caaaggagat cgcttttacg atctgatttc cgcactgcat
    23761 aagtcggtac gtggtagcgc acccgatgcg gcgctgtact ggtatgcgcg aattattacc
    23821 gctggtggcg atccgttata tgtcgcgcgt cgctgtctgg cgattgcgtc tgaagacgtc
    23881 ggtaatgccg atccacgggc gatgcaggtg gcaattgcgg cctgggattg ctttactcgc
    23941 gttggcccgg cggaaggtga acgcgccatt gctcaggcga ttgtttacct ggcttgcgcg
    24001 ccaaaaagca acgctgtcta cactgcgttt aaagccgcgc tggcggatgc acgcgaacgt
    24061 ccggattatg acgtgccggt tcatttgcgt aatgcgccga cgaaattaat gaaggaaatg
    24121 ggctacgggc aggaatatcg ttacgctcat gatgaagcaa acgcttatgc tgccggtgag
    24181 gtttatttcc cgccggaaat agcacaaaca cgctattatt tcccgacaaa caggggcctt
    24241 gaaggcaaga ttggcgaaaa gctcgcctgg ctggctgaac aggatcaaaa tagccccata
    24301 aaacgctacc gttaatgtta tcgttgcggt aatgttgtta ctgtatccct gtggtcgcag
    24361 gctgtggcca catctcccat ttaattcgat aagcacagga taagcatgct cgatcccaat
    24421 ctgctgcgta atgagccaga cgcagtcgct gaaaaactgg cacgccgggg ctttaagctg
    24481 gatgtagata agctgggcgc tcttgaagag cgtcgtaaag tattgcaggt caaaacggaa
    24541 aacctgcaag cggagcgtaa ctcccgatcg aaatccattg gccaggcgaa agcgcgcggg
    24601 gaagatatcg agcctttacg tctggaagtg aacaaactgg gcgaagagct ggatgcagca
    24661 aaagccgagc tggatgcttt acaggctgaa attcgcgata tcgcgctaac catccctaac
    24721 ctgcctgcag atgaagtgcc ggtaggtaaa gacgaaaatg acaacgttga agtcagccgc
    24781 tggggcaccc cgcgtgagtt tgactttgaa gttcgtgacc atgtgacgct gggtgaaatg
    24841 cactctggcc tcgactttgc agccgcagtt aagttgaccg gttcccgctt tgtggtaatg
    24901 aaagggcaga ttgctcgcat gcaccgcgca ctgtcgcagt ttatgctgga tctgcatacc
    24961 gaacagcatg gctacagtga gaactatgtt ccgtacctgg ttaaccagga cacgctgtac
    25021 ggtacgggtc agctgccgaa atttgctggc gatctgttcc atactcgtcc gctggaagaa
    25081 gaagcagaca ccagtaacta tgcgctgatc ccaacggcag aagttccgct gaccaacctg
    25141 gtgcgcggtg aaatcatcga tgaagatgat ctgccaatta agatgaccgc ccacacccca
    25201 tgcttccgtt ctgaagccgg ctcatatggt cgtgacaccc gtggtctgat ccgtatgcac
    25261 cagttcgaca aagttgaaat ggtgcagatc gtgcgcccgg aagactcaat ggcggcgctg
    25321 gaagagatga ctggtcatgc agaaaaagtc ctgcagttgc tgggcctgcc gtaccgtaaa
    25381 atcatccttt gcactggcga catgggcttt ggcgcttgca aaacttacga cctggaagta
    25441 tggatcccgg cacagaacac ctaccgtgag atctcttcct gctccaacgt ttgggatttc
    25501 caggcacgtc gtatgcaggc acgttgccgc agcaagtcgg acaagaaaac ccgtctggtt
    25561 cataccctga acggttctgg tctggctgtt ggtcgtacgc tggttgcagt aatggaaaac
    25621 tatcagcagg ctgatggtcg tattgaagta ccagaagttc tgcgtccgta tatgaacgga
    25681 ctggaatata ttggctaata cccaattttt ctgaatctaa aaagcgcctg cgggcgcttt
    25741 ttttgtctcc ctttgatacc gaacaataat tactcctcac ttacacgtaa tactactttc
    25801 gagtgaaaat ctacctatct ctttgatttt caaattattc gatgtataca agcctatata
    25861 gcgaactgct atagaaataa ttacacaata cggtttgtta ctggaatcaa tcgtgagcaa
    25921 gcttgagtga gccattatga aaacgaaaat ccctgatgcg gtattggctg ctgaggtgag
    25981 tcgccgtggt ttggtaaaaa cgacagcgat cggcggcctg gcaatggcaa gcagcgcgtt
    26041 aacattacct tttagtcgga ttgcgcacgc tgttgatagt gcccttccgg caaaatcaga
    26101 cgaaaaggtt atctggagcg cctgtacagt taactgtggt agtcgctgcc cgctacgtat
    26161 gcacgttgtg gacggtgaaa ttaaatatgt tgaaacggat aataccggcg atgacaacta
    26221 tgacggcctg caccaggtgc gcgcctgtct gcgtggtcgt tccatgcgtc gccgtgtcta
    26281 caatccggac cgcctgaaat atccgatgaa acgagtcggg gcgcgcggtg aaggcaaatt
    26341 cgagcgcatt agctgggaag aagcctacga catcatcgcg accaatatgc agcgcctgat
    26401 caaagagtac ggcaacgagt ctatctatct gaactatggt accggaacgc tgggcggcac
    26461 catgacccgc tcctggccgc cgggaaatac cctggtcgct cgcctgatga actgctgcgg
    26521 cggttatctg aaccattacg gtgactactc ttccgcgcaa attgcggaag gtttgaacta
    26581 tacctacggc ggctgggcag atggtaacag cccgtcggat atcgaaaaca gtaaactggt
    26641 agtgctgttt ggtaataacc ctggcgaaac gcgaatgagt ggcggtgggg tgacttacta
    26701 tcttgaacag gcgcgccaga aatctaatgc tcgcatgatc atcatcgatc cgcgctatac
    26761 cgacaccggt gccgggcgcg aagatgagtg gatccctatt cgtccgggaa cagatgccgc
    26821 actggttaac ggtctggcgt atgtcatgat caccgaaaac ctggtggatc aggcattcct
    26881 cgataaatat tgcgttggct acgacgagaa aacactgcca gccagtgcgc cgaaaaatgg
    26941 tcactataaa gcttatattc tgggtgaagg gccagatggc gtggctaaaa cgccggaatg
    27001 ggcctcgcaa atcactggtg ttccggcaga caaaatcatc aaactggctc gtgaaatcgg
    27061 tagtaccaaa ccggcgttta tcagccaggg atggggcccg cagcgtcacg ctaacggtga
    27121 aatcgcaacc cgtgctatct cgatgctggc gattctgacc ggtaacgttg gtattaacgg
    27181 aggcaacagc ggcgcgcgtg aaggttcata cagcttaccg tttgtccgta tgccgaccct
    27241 cgaaaacccg atccagacca gcatttcgat gtttatgtgg accgatgcca ttgaacgtgg
    27301 cccggaaatg acagcgctgc gtgatggtgt gcgcgggaaa gataagctgg atgtgccgat
    27361 caaaatgatc tggaactatg ccggtaactg cctgattaat cagcattctg aaatcaaccg
    27421 tacccatgaa atccttcagg atgataagaa gtgcgagctg attgtggtta tcgactgcca
    27481 catgacctca tcggcgaaat atgctgacat cctgctgcct gactgcaccg cttcagaaca
    27541 gatggacttt gcactggatg catcctgcgg gaatatgtct tacgtgattt tcaacgatca
    27601 ggtgattaaa ccgcgctttg aatgtaagac catctatgaa atgaccagcg aactggcaaa
    27661 acgtcttggc gttgagcaac agtttactga gggccgtacc caggaagagt ggatgcggca
    27721 tctgtatgcc cagtcgcggg aagcgattcc tgaactgcca acgtttgaag agttccgtaa
    27781 gcaggggatc tttaaaaagc gcgacccaca agggcatcac gttgcttata aagctttccg
    27841 tgaagatccg caggcaaatc cactgaccac gccatcgggg aaaattgaga tttattcgca
    27901 ggcgctggct gacattgccg ctacctggga attgccagaa ggtgatgtga tcgatccact
    27961 gccgatctac acgccgggct ttgaaagtta tcaggatccg ctgaacaaac agtatccgct
    28021 gcagcttacg ggtttccact ataaatctcg cgttcactca acttacggca acgttgatgt
    28081 gctgaaagca gcttgccgtc aggaaatgtg gatcaacccg cttgatgcgc aaaaacgcgg
    28141 tatcaacaac ggcgataaag tcaggatctt taacgatcgt ggtgaggttc atattgaggc
    28201 gaaagtgacg ccacgaatga tgccgggtgt ggtcgcactg ggtgaaggtg cctggtatga
    28261 cccggataca aaacgtgtcg ataagggcgg ttgtatcaac gtactgacca ctcaacgtcc
    28321 gtctcctctc gctaagggga atccgtcaca tacaaacctg gttcaggttg aaaaggtgta
    28381 aggagtaacc gatgacaacc cagtatggat tttttattga ttccagccgt tgcaccggtt
    28441 gcaaaacctg cgagctggct tgtaaagact acaaagattt gacgccagaa gtcagtttcc
    28501 gccgcattta tgaatatgct ggcggcgact ggcaggaaga taacggtgtc tggcaccaga
    28561 acgtgtttgc ctactatctg tcgatttcat gtaaccactg cgaagatccg gcttgtacta
    28621 aagtctgccc gagcggtgcg atgcataaac gtgaagatgg ttttgttgtg gtcgatgaag
    28681 atgtgtgcat tggctgccgt tactgccata tggcttgccc gtacggcgca ccgcaatata
    28741 acgaaacgaa aggccatatg accaaatgcg atggttgtta tgaccgtgtt gcggagggga
    28801 aaaagccgat ctgtgttgaa tcttgcccgc tgcgggcgct ggatttcggc cctatcgacg
    28861 agctgcgtaa aaaacatggc gatctggcgg ccgttgcgcc gttgccgcga gctcacttta
    28921 ccaaaccgaa tattgtgatc aaacccaatg ccaacagccg cccgaccggg gataccactg
    28981 gctatctggc aaacccgaag gaggtgtgag atgggaagtg gatggcatga atggccgctg
    29041 atgatcttca cggtcttcgg gcaatgtgta gcgggtggtt ttatcgttct ggctttggcg
    29101 ctgctcaaag gcgacctgcg agcagaagcc cagcagcgtg ttattgcctg catgtttggt
    29161 ttatgggtgc tgatgggcat tggctttatc gcctctatgc ttcatcttgg ttcaccgatg
    29221 cgcgccttta actcgctcaa ccgggtaggg gcttcagcac tcagtaacga aatcgccagc
    29281 ggttcgatct tttttgctgt tggtggcatc ggctggttgc tggcaatgct gaaaaagctg
    29341 tcaccggcat tgcgtacgct gtggctgata gtgacgatgg ttcttggcgt catctttgtc
    29401 tggatgatgg tgcgtgtgta taacagcatt gataccgttc cgacctggta cagcatctgg
    29461 acgccgatgg gcttcttcct gacgatgttc atgggcggcc cgttactggg ttacctgttg
    29521 ctgagtctgg ctggcgtcga tggctgggcg atgcgtctgc tgccagccat ttctgtactg
    29581 gcactggtag tgagtggcgt gatgtcagtg atgcagggcg cagagctggc gactattcat
    29641 agctctgtgc agcaggccgc agcgctggtg ccggactatg gcgcgctgat gtcctggcgg
    29701 atcgtgcttt tggctgttgc cctgtgcttg tggattgcac cacagctaaa aggttatcag
    29761 cctgcggttc cgctactttc tgtatcattc attctgcttc tggcagggga attaatcggt
    29821 cgtggcgtat tctatggttt gcatatgacc gtggggatgg ctgtcgcaag ttaatcagta
    29881 acaaccgggg tttcggcccc ggttcgcttt tatttctgct tcgttaacgt gtcataactg
    29941 gtcatcaagt tacgataatc aggaatgtgg ttagagaaca acgtcgccag tccttcaata
    30001 tcattacgcc agtcgcgatg cagctcgcat gccacgccga accatgtcat caattgcgca
    30061 ccagcttgcg acatgcgatc ccatgccgaa tgccgggtaa tttcattaaa ggtccctgac
    30121 gcgtcagtca cgacaaagac gtcaaaccct tcttcaatgg ctgaaagcgc cgggaatgcc
    30181 acgcaaactt cggttaccac accggcaata attaactgtt ttttacctgt cgctttgaca
    30241 gcttttacaa aatcttcgtt atcccaggcg ttaatatttc ccgggcgagc aatgtaaggt
    30301 gcatcgggga attgtgcttt cagttctgga actaatgggc cgttaggacc ggtttcaaaa
    30361 ctggtggtaa gaatagttgg taagttgaaa tacttagcca ggtcacccag cgccagcacg
    30421 ttatttttaa acttatcggg ttcgatatcc cgtacaaggg aaagtaaacc agcctggtga
    30481 tcaacaagca aaacggcagc atcattttta tcaagacgaa catacggttt ggtcatcctc
    30541 ttctcctttc gagagtcagt ggtacgtctg aaatcggtca gacgcgatgc attgctctga
    30601 aagcatagac gggaaatatg agtttgctgt gaccatgaaa tttttcgact gaacgaagcg
    30661 tatgaaattt gtgttagatc aataaaaaca atcagatgag attaatcgtc tctccagcat
    30721 tagcgttatg aatgagtaat ataaatcgat aatacctccg ctgcgatatg cgcgtatttt
    30781 cacccattga caatgttttt ggcggtggca tgatgcgcat gaaatttgaa cttcctcacg
    30841 gttttaattc atgtccacgt atacccggcc tgtcatgctt ttgctgtctg gcctgctttt
    30901 gttgactctg gcgatcgcag tgttaaatac actcgtgccg ctttggctcg cccaggaaca
    30961 catgtccaca tggcaggtag gcgttgtcag ctcatcctat tttaccggca acctggtcgg
    31021 tacattgctg acagggtatg tcattaagcg cattggcttt aaccgcagct attatctggc
    31081 ctccttcatt tttgccgctg gctgtgccgg ccttggcctg atgattggat tctggagctg
    31141 gttggcttgg cgttttgtcg ccggtgtcgg ttgtgccatg atttgggtgg ttgttgagag
    31201 cgcgctgatg tgcagtggga cgtcacgtaa ccgtgggcgt ttgcttgctg cgtatatgat
    31261 ggtttattac gtgggaacgt ttttaggcca gttactggtc agcaaagttt caaccgagct
    31321 gatgtccgta ttgccgtggg ttacaggttt gacgttggca gggatcttac cgctgttgtt
    31381 tacgcgtgtg ctgaatcagc aggctgaaaa ccatgattcg acgtcaatta cgtcaatgct
    31441 aaaactccgt caggcgcggc ttggcgtgaa tggctgcatt atctcaggaa tcgtgctggg
    31501 atctctatat ggcctgatgc cgctgtacct caatcacaaa ggggtgagca atgccagcat
    31561 tggtttctgg atggcggtac tggtcagtgc gggtatcctc ggacaatggc cgatcggacg
    31621 tctggcggat aagtttggtc gactgctggt gttgcgtgtt caggtctttg tcgtcattct
    31681 cggcagtatc gcgatgctta gccaggcggc gatggcccca gcgttattca tcctcggtgc
    31741 cgctggcttt acgctatatc cggtggcgat ggcctgggct tgtgagaaag ttgaacatca
    31801 tcaactggtg gcgatgaacc aggccttact gttgagctat accgtgggaa gtctgcttgg
    31861 cccgtcattt accgctatgc taatgcagaa tttctccgat aatttattgt ttatcatgat
    31921 cgccagcgta tcgtttatct atttgctgat gctgcttcgc aacgccggtc atacgccgaa
    31981 acccgttgct cacgtgtaaa tgaattcaag cagagtgtga acttactgtt tcacactctg
    32041 cttttttgtt tcttctatct gacttgcttt attccaaatt ttattcgttt aaaaataaaa
    32101 tgtgcagcag gttataattt tgcatttcgc tatttccgca cttcttattt gccgcgcata
    32161 atccctcgtt ttacagatgc ccctttaatt ttggcgaagg atttgtctat ggctgggaat
    32221 gttcaggaaa aacagctgcg atggtacaac attgcgctga tgtcttttat cactgtctgg
    32281 ggttttggca atgttgttaa taactacgcc aaccaggggc tggtggttgt tttttcatgg
    32341 gtgtttatct ttgcgctcta tttcacacct tatgcgctaa ttgttggtca gttaggctcg
    32401 accttcaaag atgggaaggg cggggtcagt acctggatta aacacacgat gggacccgga
    32461 ctggcttacc tcgccgcgtg gacctactgg gtagtgcata ttccctatct ggcacaaaaa
    32521 ccccaggcaa tactgattgc gctcggttgg gcgatgaaag gcgacggttc gttaatcaaa
    32581 gaatattcag tcgtagcgtt acaggggtta acgctggtgc tgtttatctt ctttatgtgg
    32641 gttgcttcac gcggtatgaa atcgctgaaa atcgtcggtt ctgtggcagg gattgcgatg
    32701 tttgttatgt cgctcctgta tgtggcgatg gcggtaaccg cgcctgcaat tactgaagtg
    32761 catattgcga ccacaaacat tacctgggaa acgttcattc ctcatatcga ctttacctac
    32821 attaccacta tttcaatgct ggttttcgcg gttggcggag cagagaagat ttctccttac
    32881 gttaatcaaa cgcgcaaccc aggaaaagaa tttccaaaag ggatgttatg cctggcggtg
    32941 atggttgcgg tttgtgccat tctgggttca ctggcgatgg ggatgatgtt tgactcgcgt
    33001 aatatcccgg atgacttaat gactaacggt cagtattacg cctttcagaa gctgggcgag
    33061 tattacaaca tgggtaatac tttaatggtg atttacgcca ttgcgaatac cctgggacaa
    33121 gtagctgcgc tggtattctc gattgatgcc ccgcttaaag tgctattagg cgatgctgac
    33181 agcaaatata ttccagccag tttatgtcgt accaacgctt ctggtacgcc cgttaatggc
    33241 tattttctga ccctggtgct ggtggcgatc ctgataatgc taccgactct cggcattggt
    33301 gatatgaaca atctctataa atggctgttg aatcttaact cggtagtgat gccgctgcgt
    33361 tatttatggg tatttgttgc atttattgca gttgttcgcc tggcgcagaa atataagccg
    33421 gaatatgtct ttattcgtaa taagccgctg gcaatgaccg tcggaatttg gtgttttgcc
    33481 tttaccgctt ttgcctgcct gactggtatt ttcccgaaaa tggaagcctt caccgcagag
    33541 tggacattcc agttggcgct gaatgttgca acgccgtttg tgctggtagg attagggctg
    33601 atattcccgc tgctggcgcg taaagcgaat agcaaataat tattgtggtc ggtgatgctg
    33661 actctggcag atctgacccg tttttacata atcgagaaat gcgcgcaagc cgcaggacat
    33721 atactggcgg tttgggtaat acatttggaa gctgtgctgt aagggcgtta gaaatagagc
    33781 tctgaattat ttatgcagcg cggaataaaa aagcggcaac tcaaaaaagt tgccgcttta
    33841 cggggaaatt agaacattac cttatgaccg tactgctcaa gaatgccttt cacgcgttcc
    33901 atggtctctt tcttcggtgg tttaacacca tcaagtttgt attcttcacc catcgccacc
    33961 catttgtgtt tgcccagttc gtggtagggg agaagctcga ttttctcaac gttgcccata
    34021 tcacgggtaa attcaccgag gcgatgcgct gaatcgtcat cgtcagacca gcctgggaca
    34081 acaacgtagc ggatccacac cttcacattt ttgttcgcca gatatttggc gaactccagc
    34141 gtgcggtggt tggaaactcc aaccagattt tggtggatct cgtcgttcat ctgtttgaga
    34201 tcgagcatta ccaggtcggt tacttccagc agttcatcaa tcaccggatc gtaacgacga
    34261 acaaaaccgt tggtgtccag acaggtatga atgccttctt ttttgcaggc gcggaaccag
    34321 tcacgaacaa actcagcttg caggattgct tcaccgccgg atgcggtaac gccgccgccg
    34381 gaagcgttca taaagtggcg ataggtcacc acctctctca ttaaatcttc aacggtaact
    34441 tctttaccgc catgcgtgtc ccaggtgtcg cggttatgac aatacaggca gcgcatcagg
    34501 cagccctgga aaaaggtgat aaagcgaata cccggaccgt ctacggttcc acaggattca
    34561 aaggagtgaa tgcgaccaat aactgacatt gcggtgtttc tccagatgtg gcccatctga
    34621 ggccgtgttg gtgcgcagct cgaaggctac gtcgagtctg ttttggcagt caccttaaag
    34681 tatagatagc tgacaaaaaa ggctctcgtg ctaaaaaagg ccccactttc gtggagcctt
    34741 tattgtacgc tttttactgt acgatttcag tcaaatctaa ttacatagat tgagtgaagg
    34801 tacgagtaat aacgtcctgc tgctgttctt tagtcagcga gttgaaacgt actgcgtagc
    34861 cagatacacg gatggtcagc tgcggatatt tttccgggtt ttccatcgcg tcgagcagca
    34921 tttcacggtt catcacgtta acgttcaggt gctgaccacc ttcgatggac gcttcgtggt
    34981 ggaagtaacc atccatcaga ccagccaggt tggtcttacg aacttcgtcg tctttaccca
    35041 gtgcgttcgg aacgatagag aaggtgtagg agataccatc tttagcgtaa gcaaacggca
    35101 gtttagcaac ggaagtcaga gaggctactg cacctttctg gtcacgaccg tgcatcgggt
    35161 tagcacccgg tccgaacggc gcgccagcac gacgaccgtc tggggtgtta ccagttttct
    35221 taccatacac aacgttagaa gtgatggtca gaacagactg agtcgggata gcgtcacggt
    35281 aggtgtgcag tttctgaatt ttcttcatga aacgttctac caggtcaaca gccaggtcat
    35341 ctacacgcgg atcgttgtta ccaaactgcg ggtattcgcc ttcgatttcg aagtcgatag
    35401 ccagaccgtc ttcgtcacga atcggtttaa ctttcgcata tttgattgca gacagggagt
    35461 cagcagcaac ggacagacca gcgataccac acgccatggt gcggataacg tcacggtcgt
    35521 gcagcgccat cagagaggct tcgtagctgt acttgtcgtg catgtagtgg atgatgttca
    35581 gtgcagtgat gtactgttta gccagccagt ccatgaagtg atccatgcgc tccatcactt
    35641 catcgtagtt caggagatcg cctttgatcg gctcagactt cggaccaacc tgcattttca
    35701 gtttttcgtc aacgccgccg ttgattgcgt acagcatggt tttcgccagg tttgcacgcg
    35761 caccgaagaa ctgcatttgt ttaccaacga tcatcgggct tacgcagcaa gcgatagcgt
    35821 agtcatcgtt gttgaagtcc ggacgcatca ggtcatcgtt ctcatactgc agagaagagg
    35881 tgtcgatgga cactttagca gcgaatttct tgaagttcag cggcagtttt tcggaccaca
    35941 gaatggtcat gttcggttcc ggagacggac ccatggtgta cagggtgttc aggaaacgga
    36001 agctgttttt ggtaaccagg gtacgaccgt cgaggcccat accaccgata gattcggttg
    36061 cccagatcgg gtcgccagag aacagttcat cgtattccgg agtacgcagg aagcgaacca
    36121 tacgcagttt catgaccagg tggtcaacca tttcctgcgc ttcttgttcg gtgatcttgc
    36181 cagctttcag gtcacgttcg atgtacacat ccaggaaggt ggaggtacga ccgaaggaca
    36241 ttgcagcacc gttctgagac ttaacagcag ccaggtagcc gaagtaagtc cactggatag
    36301 cttcctgagc gttggtagcc ggaccagaga tgtcgtagcc gtatttcgca gccatttctt
    36361 tcatctgacc cagagcgcgg tgctgttcag cgatttcttc gcgcagacgg atagtctgtt
    36421 ccaggtttac gccgttttcc agatcagcct gcagagaggt gaactgagcg tatttgtctt
    36481 tcatcagata gtcgataccg tacagcgcaa cgcgacggta gtcaccgatg atacgaccac
    36541 ggccataagc atctggcaga ccggtcagaa caccggattt acggcaacgc aggatgtccg
    36601 gagtgtaaac gtcgaacacg ccctggttgt gagttttacg gtattcagtg aagatttttt
    36661 tgatcatcgg gtccagttcg cggttgtacg ctttgcagga accttcgatc attttgatac
    36721 caccgaacgg gataagagca cgtttcagcg gagcttcagt ctgcagacca acaacttttt
    36781 ccaacgcttt gttgatgtag ccagcgtcgt gagaggtgat ggtggaagca acagcggtgt
    36841 caaagtcaac tggcgcgtga gtgcggtttt ccagtttaac gccttccatt actttgtccc
    36901 acagggtggt ggtcgcttca gtagcgccag ccaggaagga ctcgtcaccc tcgtacggag
    36961 tgtagttttt ctgaatgaag tcacggacgt ttacttcatt ctgccagtca cctttggtaa
    37021 aaccttccca ggctgtggct aacttttcat taagctcgga catgtaacac ctaccttctt
    37081 aagtggattt tttatttact gcgtacttcg acaaccatta atggtgatcg ttttcacgca
    37141 gataaatgac ccagtatgtc aacccaacca acaaaccacc gccgataata ttaccgatcg
    37201 taaccggaat caggttatca gtgatgaagt tcatcacggt caggtgagaa aaattttccg
    37261 gtgcagaacc gacagcggtc cagaattccg gagatgcgaa gtcgcggatt acaataccca
    37321 tcgggatcat aaacatgttt gcgatactgt gctcaaaacc gctggcaaca aacatcgcga
    37381 ccggcagcac cataatgaac gctttatcca tcaggctgcg gccagaataa ctcatccata
    37441 ctgccagaca taccatcagg tttgccagga taccaagaca gacggcctca ataaaagtat
    37501 ggtgcacttt gtggtcggcg gtttgtagga cgtttagtcc ccattgacca ttcgcggtca
    37561 tatactcgcc ggaaagccac attaaaagta caaacagcag tgcgccgacc aggttgccaa
    37621 aatagacatt tagccagttt ttcgctaact gaccccaggt gatgcgccca ctcgccttag
    37681 caacaacaat caacactgtg gaagtaaaga gatcggctcc gcagacaaca caaagaatca
    37741 gccccagaga gaagcaaatg ccgccaacca gttttgccat gccgaagggc attgttcctg
    37801 tgccagtggt tgctgtgata tagaagacga atgcgattga gatgaaaaca ccggcggtaa
    37861 tcgccagata gaaagtctta agcggatgtt tcgttgcttt atagacacct gcctcttcgg
    37921 ccactttggc cattgctgca ggaagtaaaa gatcaaaagg gttgtcagct ttcacactaa
    37981 ctctctcttt attaagtcgg cgacgagata ctaacaaagc attatagatg agaaattgat
    38041 atagatcata tctcgcctgg cttatagg
//
LOCUS       DAZGQL010000045        37517 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.045, whole genome shotgun sequence.
ACCESSION   DAZGQL010000045 DAZGQL010000000
VERSION     DAZGQL010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37517
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.045"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(60..341)
                     /gene="yehE"
                     /locus_tag="ACHKIR_002520"
     CDS             complement(60..341)
                     /gene="yehE"
                     /locus_tag="ACHKIR_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HFO9990244.1"
                     /translation="MNKYWLSGIIFLAYGLASPAFSSETATLTINGRISPPTCSMAMV
                     NGQPQQHCGQLTYNVDTRHQVSSPVKGVTTEVIAADSDSKRRIVLNRYD"
     gene            complement(604..1713)
                     /gene="apbC"
                     /locus_tag="ACHKIR_002521"
     CDS             complement(604..1713)
                     /gene="apbC"
                     /locus_tag="ACHKIR_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HFO9990245.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPNMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            1845..3878
                     /gene="metG"
                     /locus_tag="ACHKIR_002522"
     CDS             1845..3878
                     /gene="metG"
                     /locus_tag="ACHKIR_002522"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFO9990246.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEEIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKATTAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            4020..7808
                     /locus_tag="ACHKIR_002523"
     CDS             4020..7808
                     /locus_tag="ACHKIR_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HFO9990247.1"
                     /translation="MRHFIYQDEKSHKFWAVEQQGNELHINWGKVGTNGQSQVKSFAD
                     AAAAEKAGLKLIAEKVKKGYVEQAKDNSLQPSQTVTDSLKVADLSTIIQEQPSFVAET
                     RAADKNTDAVLPWLAKDIAVVFPPEVVHTTLSHRRFPGVPVQQADKLTQLRRLACSVS
                     QRDNKTATFDFSACSLEWQNTVAQAISQIDGLKTTQLPSPVMAVLTALEMKCTRYKER
                     EDVMDQIIQEGGLEYATDVIIHLQQISIEWDYVNNNIVFLSSGISPDYLQQYSSFELR
                     LRKHLSLAEESLWQKCAQKLIAAIPHIPEWRQPLIALLLPEKPEIAHEISQRLLGQKK
                     LPSLEWLKIVATDEHILASLEKYHEPYAIFDDYYCGAIWSATVLQEQGVTALPRFAPY
                     AASDYCADVLRHINHPFALTLLIRVAGHTKRCHDRMTKACAAFPHAALAALAELLVQK
                     EENSWRIMLMTMLISHPTLAEQVIPWLSTPAVAVLKSCQQQLTQPSNHASADLLPAIV
                     VSPPWLSKKKKSPIPVLDLAPLNLESICTITDTEAKEFQTHWDWEPHKPSEGAKNFLY
                     SLGYRRWDFDTYKYIGASDSAIDAWEREDFATLIQMFKAHHAPYQGEWHLNSLPFLPM
                     QKAIKLWEFLSKEPHTAIKPVMLYLRLAGMSGFLHSFSRYPQEGFAVANYFAATELAP
                     AVARAFNKLKTLRQDASSWLLKYPEHAITGLLPAALGKASEAQDNARAALRMLTENGH
                     QPLLQEIARRYNQPEVTDAVNALLALDALDNHPTKIPTLPTFYQPSLWTRPLLKANAQ
                     SLPDSALLHLGEMLRFPQEEALYPGLLQVKDACTTDSLAEFAWDLFTAWQTAGAPSKE
                     SWAFTALGVLGNDDTARKLTPLIRAWPGESQHKRATVGLDILAAIGSDIALMQLNGIA
                     QKLKFKALQERAKEKIADIAESRELTVAELEDRLAPDLGLDDNGSLLLDFGPRQFTVS
                     FDETLKPFVRDASGSRLKDLPKPNKSDDELQANNAVNRYKLLKKDARTVAAQQVARLE
                     SAMCLRRRWSPENFQLFLVEHPLVRHLTRRLIWGVYSAENQLLACFRVAEDNSYSTAD
                     DDLFTLPEGDISIGIPHVLEISPTDAAAFGQLFADYELLPPFRQLDRNSYALTEAERN
                     ASELTRWAGRKCPSGRVMGLANKGWMRGEPQDGGWIGWMIKPLGRWSLIMEIDEGFAV
                     GMSPAELSAEQLLSKLWLWEGKAESYGWGSNSTQEAQFSVLDAITASELINDIEALFE
                     "
     gene            7818..11450
                     /gene="yehI"
                     /locus_tag="ACHKIR_002524"
     CDS             7818..11450
                     /gene="yehI"
                     /locus_tag="ACHKIR_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein YehI"
                     /protein_id="HFO9990248.1"
                     /translation="MDKELPWLADNAQLELKYKKGKTPLSHRNWPGEPVPVITENIIQ
                     TLGDELLQKAEKKKNIVWRYENFSLEWQSAITQAINLIGEHKPSITARTMAALACIAQ
                     NDSQQLLDEIVQHEGLEYATEVVIARQFIVRCYESDPLVVTLQYQNEDYGYGYRSETY
                     NEFDLRLRKHLSLAEESCWQRCADKLIAALPGITKVRRPFIALNLPEKPEIANELVSL
                     ECSQTHFRSKEWLKVVADDPKAVKELARYWSQDIFSDREASYMSHENHFGYAACAALL
                     REQGLAAVPRLAMYAHKEDCGSLLVQINHPQVIRTLLLVADKNKPSLQRVAKYSKNFP
                     HATLAALAELLALKAPPARPGYPIIEDQKLPAQQKARDEYWHTLLQTLMASQPQLAEE
                     VMPWLSTQARAVLNSYLSAPPKTVIDSTDNSQMPEILVSPPWRSKKKMTAPRLDLAPL
                     ELTPQVYWQPGEQERLASTESARYFSTESLAERMEQKSGRVVLQELGFGDDVWLFLNY
                     ILPGKLDAARNSLIVQWHYYPGRVEEIMNGWSSPEAQLAEQALRSGHVEVLINIWEND
                     SYSRYRPEKSVWNLYLLAQLPREMALPFWLRINEKKHLSAGEDYFLSIFGLDALPGLL
                     LAFSHRPKETFPLILNFGATELALPVARVWRRFAAQRDLARQWILQWPEHTATALIPL
                     VFTKPSDNSEAALLALRLLYEQGHGELLQTAANRWQRTDVWPALEHLLKQSPIEIYPA
                     RMPKAPDFWHPLMWSRPRLIINHQLITDDALEIIGEMLRFTQGGRFHSGLEQLKTFCQ
                     PQTLAAFAWDLFTAWQQAGAPAKDNWAFLALSLFGDESTARDLTTQILAWPQEGKSTR
                     AVSGLNILTLMNNDMALIQLHHISQRAKSRPLRDNAAEFLQVVAENRGLSQEELADRL
                     VPTLGLDDPQALSFDFGPRQFTVRFDENLNPVIFDQQNVRQKSVPRLRTDDDQLKAPE
                     ALARLKGLKKDTTQVSKNLLPRLEAALRTTRRWSLADFHSLFVNHPFTRLVTQRLIWG
                     VYPANEPRRLLNAFRVAAEGEFCNAQDEPIDLPADALIGIAHPLEMTEGMRSEFAQLF
                     ADYEIMPPFRQLARRTVLLTPDESTSNSLTRWEGKSATVGQLMGMRYKGWESGYEDAF
                     VYGLGEYRLVLKFSPGFNHYNVDSKALMSFRSLRVYRDNKSVTFAELDVFDLSEALSA
                     PDVIFH"
     gene            11512..11865
                     /locus_tag="ACHKIR_002525"
     CDS             11512..11865
                     /locus_tag="ACHKIR_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000636928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9990249.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCSELFTGSVYRLER
                     FRLRPTFHQRDREDADPLINDALIYIRDECIDERKLRGESPETVIAIFNRKLQNIFNQ
                     LIEYYFIKGGSGFPP"
     gene            13013..14101
                     /gene="yehL"
                     /locus_tag="ACHKIR_002526"
     CDS             13013..14101
                     /gene="yehL"
                     /locus_tag="ACHKIR_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HFO9990250.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDHAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATAISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
     gene            14112..16391
                     /gene="yehM"
                     /locus_tag="ACHKIR_002527"
     CDS             14112..16391
                     /gene="yehM"
                     /locus_tag="ACHKIR_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
                     /protein_id="HFO9990251.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGAWTPYAEFSPEWQALQAARRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQEESQTLLLRATRMDNSDTLWDHLFEDESQQTALPSALAHYFA
                     QLRGDSPGDALNRQREDFMARWIGWAMQQNNGDVLVVCGGWHASALAKMWRECPQEIN
                     TPELPSLADAVTGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWRWGLQQAGEQLLKT
                     VLTRLRQHHLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTGISFPAE
                     LTLNRFTPDGLAQSQVLHRLVILEIPGVVRQQGSTLTLAGNGEERWKLTRPLSQHAAL
                     IEAACFGATLQEAARNKLEADMLDAGGIGSITTCLSQAALAGLASFNQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVNLPGVSLSAAVALLERRSQAIHAPALDRGAALGAL
                     MRLEHPNASAEAALTMLVQLSPTQSGEALHGLLALARHQLACQPVFIAGFSSHLNQLS
                     DADFINALPDLRAAMAWLPPRERGTLAHQVLEHYQLVQLPVSALQMPLHCPPQDIAHH
                     QQLEQQALASLQHWGVFHV"
     gene            16384..17520
                     /gene="yehP"
                     /locus_tag="ACHKIR_002528"
     CDS             16384..17520
                     /gene="yehP"
                     /locus_tag="ACHKIR_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFO9990252.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGDSNLTTPEWINSIHTLFPQRVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRHSDQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDMAQALVNVGAQIAAMTPGELATWLAENLQS"
     gene            17517..19520
                     /gene="yehQ"
                     /locus_tag="ACHKIR_002529"
     CDS             17517..19520
                     /gene="yehQ"
                     /locus_tag="ACHKIR_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFO9990253.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     GALIATFSDGVRTQLANGQALKEAQCSCGASGMCRHRVMLVLSYQRLCATTQPTEKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKGITIELFCTPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIDGTLCEHVVLAVQAFVEAKTQQAEFTHLIWQMRSEHVTSSDDPFASEEG
                     NACRQYVQQLSQALWLGGISQPPIHYEAAFSRAQQAAERCNWRWVSESLRQLRASVDA
                     FHARASHYHAGKCLCQLAALTSRLNSAQEIARRDSVGEVPPMPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASASAPYAVERMAALLQQTDDPVCLVSGFVSFVEGQLTLEPQVMMTKTRAWALDAET
                     APVVASLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEHSAIGQAELLANDLTA
                     VGFYRLAHVLGQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            19645..20106
                     /gene="yehR"
                     /locus_tag="ACHKIR_002530"
     CDS             19645..20106
                     /gene="yehR"
                     /locus_tag="ACHKIR_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HFO9990254.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAARTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(20148..20618)
                     /gene="yehS"
                     /locus_tag="ACHKIR_002531"
     CDS             complement(20148..20618)
                     /gene="yehS"
                     /locus_tag="ACHKIR_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFO9990255.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(20665..21384)
                     /gene="btsR"
                     /locus_tag="ACHKIR_002532"
                     /gene_synonym="yehT"
     CDS             complement(20665..21384)
                     /gene="btsR"
                     /locus_tag="ACHKIR_002532"
                     /gene_synonym="yehT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HFO9990256.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(21381..23066)
                     /gene="btsS"
                     /locus_tag="ACHKIR_002533"
     CDS             complement(21381..23066)
                     /gene="btsS"
                     /locus_tag="ACHKIR_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HFO9990257.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDCYTRITLRLPWRDEA"
     gene            23288..24019
                     /gene="mlrA"
                     /locus_tag="ACHKIR_002534"
     CDS             23288..24019
                     /gene="mlrA"
                     /locus_tag="ACHKIR_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HFO9990258.1"
                     /translation="MALYTIGEVALLCDINPVTLRAWQRRYGLLKPQRTDGGHRLFND
                     ADIDRIREIKRWIDNGVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRT
                     WIKERGQDYPAQTLTTHLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARKK
                     QGKDALVVGWNIHDTTRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGE
                     NRTSAQQQQLTSWQEQGHDIFPLGI"
     gene            24079..24186
                     /gene="yohO"
                     /locus_tag="ACHKIR_002535"
     CDS             24079..24186
                     /gene="yohO"
                     /locus_tag="ACHKIR_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HFO9990259.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(24167..24898)
                     /gene="yehW"
                     /locus_tag="ACHKIR_002536"
     CDS             complement(24167..24898)
                     /gene="yehW"
                     /locus_tag="ACHKIR_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HFO9990260.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIIVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKSMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(24903..25829)
                     /gene="yehX"
                     /locus_tag="ACHKIR_002537"
     CDS             complement(24903..25829)
                     /gene="yehX"
                     /locus_tag="ACHKIR_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HFO9990261.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLEPNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGHSELGVRLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGEPCGTLHFQDLLVEA"
     gene            complement(25822..26979)
                     /gene="yehY"
                     /locus_tag="ACHKIR_002538"
     CDS             complement(25822..26979)
                     /gene="yehY"
                     /locus_tag="ACHKIR_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HFO9990262.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACVIPGKKGSICALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHLLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGVWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGISGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLIDALFDLLIALLKVKRND"
     gene            complement(26986..27903)
                     /gene="osmF"
                     /locus_tag="ACHKIR_002539"
     CDS             complement(26986..27903)
                     /gene="osmF"
                     /locus_tag="ACHKIR_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HFO9990263.1"
                     /translation="MPLSKLWAGSLVLLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAG
                     QGYEKVKKLDAEQNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLKEGGTF
                     KLAASAEFIERADALPAFEKAYDFKLDQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWTK"
     gene            complement(28052..30349)
                     /gene="bglX"
                     /locus_tag="ACHKIR_002540"
     CDS             complement(28052..30349)
                     /gene="bglX"
                     /locus_tag="ACHKIR_002540"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HFO9990264.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSTMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMEELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNAV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATAKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            30545..32260
                     /gene="dld"
                     /locus_tag="ACHKIR_002541"
     CDS             30545..32260
                     /gene="dld"
                     /locus_tag="ACHKIR_002541"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFO9990265.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEYLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGVGKTSKRKN
                     WQEVE"
     gene            complement(32299..33231)
                     /gene="pbpG"
                     /locus_tag="ACHKIR_002542"
     CDS             complement(32299..33231)
                     /gene="pbpG"
                     /locus_tag="ACHKIR_002542"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HFO9990266.1"
                     /translation="MPKFRVSLFSLALMLAVPLAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(33405..33992)
                     /gene="yohC"
                     /locus_tag="ACHKIR_002543"
     CDS             complement(33405..33992)
                     /gene="yohC"
                     /locus_tag="ACHKIR_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HFO9990267.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            34162..34740
                     /gene="yohD"
                     /locus_tag="ACHKIR_002544"
     CDS             34162..34740
                     /gene="yohD"
                     /locus_tag="ACHKIR_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFO9990268.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILAVVLVVGVRWWLKRRGKKKPDNQA"
     gene            complement(34859..35620)
                     /gene="yohF"
                     /locus_tag="ACHKIR_002545"
     CDS             complement(34859..35620)
                     /gene="yohF"
                     /locus_tag="ACHKIR_002545"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO9990269.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGARAEIVQLDLGKLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKAMALELVRHKILVNAVAPGAIATPMNGMDDSDVRPDAEPSIPLRRFGTTHEIA
                     SLVVWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(35673..37109)
                     /gene="mdtQ"
                     /locus_tag="ACHKIR_002546"
     CDS             complement(35673..37109)
                     /gene="mdtQ"
                     /locus_tag="ACHKIR_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HFO9990270.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQHTPAVQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPVMQVAEQRIQLAEAQAKAIATQD
                     GPQLDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLSALTNHQTKALKLNP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVDAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
     gene            37333..37416
                     /gene="yohP"
                     /locus_tag="ACHKIR_002547"
     CDS             37333..37416
                     /gene="yohP"
                     /locus_tag="ACHKIR_002547"
                     /inference="COORDINATES: protein motif:HMM:NF011345.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YohP"
                     /protein_id="HFO9990271.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
BASE COUNT         9268 a        10013 c         9386 g         8850 t
ORIGIN      
        1 taaatcattc atgtgaatga tttaagcttt ctaaatccga ttaaaaccag ggtggcaaat
       61 taatcgtagc gatttaacac gattcgacgt ttgctatcgc tatctgcagc aatcacttcc
      121 gtcgttacac ctttaactgg cgaagaaacc tgatgacggg tatcaacatt atacgttaac
      181 tgaccacaat gctgttgagg ttgaccatta accatagcca tactgcatgt cgggggactg
      241 atcctaccat taatcgttaa tgtggcagtt tctgaagaga acgcgggaga ggccaatcca
      301 taggcaagaa aaataattcc tgacagccaa tacttattca ttgaacgtta tccctgtagt
      361 aaaggttatg cctggataga atgagtgcat aacaaactat agctgtacat ccactacaca
      421 gccacgaagg atgataatga agcattgcct gtacgatcaa tcgactttgt agagtttcgg
      481 acgaaggtcc gcagaatatt cgcagcatta aataagtgtt cattaatgcg gcaaaaacaa
      541 atacaccgca tcaatgtaac atctctgcgg tgtatgaaga ataaccagaa tggttaatac
      601 gcgttagacc gcgcggaagg aaatctcgcc tggaatgact tcaccctgcc agtaaagctg
      661 agctgcaacg cggtcagcca gctggcggta gatagcggta aattcgctct caggacggct
      721 gataaccgtt ggcgtgcctt tatcgagatc ctcacgtaag gagatgtgta acggcatctg
      781 ccccagcagc tgtgtgtggt atttctcggc cagtttctct gcaccaccgg taccgaaaat
      841 cggttcatgg tgaccacagt tactgcaaat atgcacactc atgttctcga caataccgag
      901 caccggaacc tcgactttct cgaacatcac aatgcctttt ttcgcatcga tcaacgcgat
      961 gtcctgcggg gtggtgacca cgactgcacc ggttactgga atgttttgtg ccaatgtcaa
     1021 ttgaatgtca ccggtacctg gcggcatatc aagcaccaga tagtcgagat ccggccacaa
     1081 ggtttcctgc aacatttgca ttaacgcctt gctcgccatt gggccacgcc acaccatcgc
     1141 attgtcgtct gtcaccagat aaccaattga attagttgcc aggccatgag acataatcgg
     1201 tgccatatgg gtaccgtccg gcgaagttgg acgctgattt tctgcaccca gcatgttagg
     1261 gattgatggg ccgtagatat cggcgtccag aatgccgact ttcgcccctt ccgccgccag
     1321 tgccaacgcc aggtttaccg ccgtggagga tttacccacg ccacctttac cggaactgac
     1381 cgcgataatg ttcttcacgc cgttaattcc cggctgattc ttcacgcgtt tcagagtggc
     1441 tatgttatgc gacagtttcc agtcgatagc tttcgctccg gtgatgcgca gcagttcggc
     1501 gctacattgc tcttttaact cttcgaacgc gctatgccag acgaatggca taaccagttc
     1561 cacatgcagc gtgtcgtcca tccaggcgac atggtgcaac gctttcagcg ttgtgaggtt
     1621 atgcttcagg gttgggtgct gaaaattggc cagtgtcccg gcgaccattg cgcgcaaggc
     1681 ttctggtgat ttggcctggg attgttcgtt catcccgact ccttttttgt atagataaac
     1741 catcagttga tagtttacct gaagaatata gagaagtact tacttaacat tttcccattt
     1801 ggtactatct aagccctttt cactattaag aagtaatgcc tactatgact caagtcgcga
     1861 agaaaattct ggtgacgtgc gcactgccgt acgctaacgg ctcaatccac ctcggccata
     1921 tgctggagca catccaggct gatgtctggg tccgttacca gcgaatgcgc ggccacgagg
     1981 tcaacttcat ctgcgccgac gatgcccacg gtacaccaat catgttgaaa gctcagcagc
     2041 ttggtatcac tccggagcag atgattggcg aaatgagtca ggagcatcag actgatttcg
     2101 caggctttaa catcagctat gacaactatc actcgacgca cagcgaagag aaccgccagt
     2161 tgtcagaact tatctactct cgcctgaaag aaaacggttt tattaaaaac cgcaccatct
     2221 ctcagctgta cgatccggaa aaaggcatgt tcctgccgga ccgttttgta aaaggcacct
     2281 gcccgaaatg taaatccccg gatcaatacg gcgataactg cgaagtctgc ggcgcgacct
     2341 acagcccgac tgaactgatc gagccgaaat cggtggtttc tggcgctacc ccggtaatgc
     2401 gtgattctga acacttcttc tttgatctgc cctctttcag cgaaatgctg caggcatgga
     2461 cccgcagcgg tgcgttgcag gagcaggtgg caaataaaat gcaggagtgg tttgaatctg
     2521 gcctgcaaca gtgggatatc tcccgcgacg ccccttattt cggttttgaa attccgaacg
     2581 cgccgggcaa atatttctac gtctggctgg acgcaccgat tggctacatg ggttctttca
     2641 agaatctgtg tgacaagcgc ggcgacagcg taagcttcga tgaatactgg aagaaagact
     2701 ccactgccga gctgtaccac ttcatcggta aagatattgt ttacttccac agcctgttct
     2761 ggcctgccat gttggaaggt agcaacttcc gcaagccgac caacctgttt gttcacggct
     2821 atgtaacggt gaacggcgcg aagatgtcca agtctcgcgg cacctttatt aaagccagca
     2881 cctggctgaa tcattttgac gctgacagcc tgcgttacta ctacactgcg aaactctctt
     2941 cgcgcattga tgatatcgat ctcaacctcg aggatttcgt tcagcgtgtg aatgctgata
     3001 tcgtcaacaa agtggttaat ctggcgtccc gtaacgcggg ctttatcaac aaacgttttg
     3061 acggcgtgct ggcaagcgaa ctggctgacc cgcaactgta caaaaccttc actgatgccg
     3121 ctgaagagat tggcgaagcg tgggaaagcc gtgaatttgg taaagcagtg cgcgaaatca
     3181 tggcactggc tgacctggct aaccgctatg tcgatgaaca ggctccgtgg gtggtggcga
     3241 agcaggaagg tcgcgatgct gacctgcagg cgatttgctc tatgggcatt aacctgttcc
     3301 gcgtactgat gacttacctg aagccggtac tgccgaaact gactgaacga gcagaagcat
     3361 tcctgaatac ggaactgacc tgggatggta tccagcaacc gctgctgggc cataaagtga
     3421 atccgttcaa agcactgtat aaccgtatcg acatgaagca ggttgaagca ctggtggaag
     3481 cgtctaaaga agaagtgaaa gccactaccg cgccagttac tggcccactg gcagatgatc
     3541 cgattcagga aaccatcacc tttgacgact tcgctaaagt tgacctgcgc gtggcgctga
     3601 ttgaaaacgc agagtttgtt gaaggttctg acaaactgct gcgcctgacg ctggatctcg
     3661 gcggtgaaaa acgcaatgtc ttctccggca ttcgttctgc ttacccggat ccgcaggcac
     3721 ttattggtcg tcacaccatt atggtggcta acctggcacc acgtaaaatg cgcttcggta
     3781 tctccgaagg catggtgatg gctgctggtc ctggcgggaa agatatcttc ctgctaagcc
     3841 cggatgccgg tgctaaaccg ggtcatcagg tgaaataatc cccccttcaa ggcgctgcat
     3901 cgacagcgcc ttttctttat aaattcctaa atttgttttc ttgcgatttt gtctctctct
     3961 aacccgcata aatactggta gcatctgcat tcaactggat aaaattacag ggatgcagaa
     4021 tgagacactt tatctatcag gacgaaaaat cacataaatt ctgggcggtg gagcagcagg
     4081 gaaacgaact gcacatcaac tggggtaaag tcggcaccaa cggacaaagc caggtaaaaa
     4141 gttttgcgga tgcagcagcg gcggaaaaag cgggactgaa gctgattgca gagaaggtga
     4201 agaaggggta tgtggagcaa gcgaaggata attctttgca accttcccaa acggtaacgg
     4261 actctctcaa ggtagctgac ttatccacca ttattcagga acaaccctct ttcgtagcag
     4321 aaacccgtgc ggctgacaaa aatacagatg ccgttttacc gtggctggcg aaagatattg
     4381 ctgtcgtttt tccgcccgaa gttgtacaca ccacgttaag tcatcgccgc tttcccggag
     4441 ttcctgttca gcaagcagac aaattgaccc aattacgtcg cttagcctgt agtgtgtcgc
     4501 aacgggataa taaaacagcc acatttgact tcagcgcctg ttctttagaa tggcaaaaca
     4561 ccgtcgccca ggcgatcagt cagatcgacg gcctgaaaac aacacagcta ccatcacccg
     4621 taatggctgt actcactgca cttgaaatga aatgcacaag atataaagag cgtgaggatg
     4681 ttatggatca gattattcag gaaggcggtc tggaatatgc tactgatgta ataatacatc
     4741 ttcaacaaat atctattgaa tgggattatg tcaacaataa tattgttttc ctgtcgtctg
     4801 gcatttcacc tgattacttg cagcaatatt ccagctttga attacgccta cgtaaacatt
     4861 tatcactggc ggaagagtct ctctggcaaa aatgtgcaca aaaacttatt gccgcaattc
     4921 cacatattcc agaatggcgg caaccattaa ttgctttgtt attacccgaa aaaccagaaa
     4981 ttgcacatga aatttcccag cgtctactgg ggcaaaagaa attaccctcg cttgagtggc
     5041 taaaaatagt ggcgactgat gagcacattc ttgcctcatt agaaaaatat cacgagccat
     5101 atgccatttt tgatgattac tattgtggtg cgatatggtc agccaccgta ttacaggagc
     5161 aaggtgttac agccctgccc cgatttgctc cctatgccgc aagtgactac tgcgccgatg
     5221 tgttgcgtca tatcaatcat ccgttcgcat taacactgct tatacgtgta gccgggcata
     5281 ctaaacgctg tcacgatcga atgacgaaag cctgcgctgc gttcccacac gcagcactgg
     5341 cagcactggc ggaacttcta gtgcaaaaag aagagaatag ttggcgcatt atgctaatga
     5401 cgatgcttat ctcgcatccg acactagcag aacaggtcat tccctggctt tcgacacccg
     5461 cagttgcagt gctgaaatca tgtcagcaac aactgacaca gccctcaaac catgccagcg
     5521 ccgatctact gccggccata gtagtctccc caccatggct ttcgaaaaag aaaaaatcgc
     5581 cgattccggt gctggattta gcaccgctca acctcgaatc catatgcaca ataaccgaca
     5641 cagaagctaa agagtttcaa actcattggg actgggaacc gcataagcca agtgaaggtg
     5701 ctaaaaattt cctttactcg ctggggtatc gccgctggga ttttgacaca tataaatata
     5761 tcggcgcttc tgactcggca attgacgcat gggaacgtga ggacttcgct acactcatcc
     5821 aaatgtttaa agcccaccat gcaccgtatc agggggagtg gcatctcaac tcacttccct
     5881 ttttacctat gcaaaaagcg attaagctat gggaatttct cagcaaagag ccgcataccg
     5941 cgataaaacc agtcatgctt tatttgcgac tggcaggtat gagtggtttt ctacactcgt
     6001 tttcacgcta ccctcaagaa ggttttgcgg ttgctaatta ttttgccgca acggaactgg
     6061 ctcctgccgt cgcccgcgcc ttcaacaaac tcaaaacatt acgacaagac gccagtagct
     6121 ggctgctgaa atacccggaa cacgccatta ccggcctgct gcctgcagcg ctcggcaaag
     6181 ccagtgaagc acaggacaac gcccgtgctg ccttgcgtat gcttaccgaa aacggtcatc
     6241 agccattgct gcaagaaatc gcccggcgtt ataaccagcc ggaagtaacc gatgcggtga
     6301 acgctctact tgcgctcgat gccttagata atcacccgac aaaaatcccc actcttccga
     6361 ccttttatca gccatcgctc tggacgcgcc cgttattaaa agcaaacgcc caatcactgc
     6421 ctgatagcgc actcctccac ctcggtgaaa tgctccgctt ccctcaggaa gaggcactgt
     6481 atccgggatt attgcaggtg aaagatgcct gtaccaccga ctcactggct gaatttgcct
     6541 gggatctgtt taccgcctgg cagaccgctg gcgcgccgtc gaaagagagt tgggcgttca
     6601 ctgcgttagg cgttctcggt aacgatgaca ccgcccgcaa actgacgcct ttgattcgcg
     6661 cctggcctgg tgaatcccag cataaacgcg ccaccgtcgg gttggatatt ctcgctgcta
     6721 tcggtagtga tatcgccctg atgcagctta acggcatcgc ccagaaactg aaattcaaag
     6781 cattacagga gcgggcaaaa gaaaaaattg ccgacattgc cgagagccgc gaactcacgg
     6841 tggcggagct tgaagatcgg ttagcaccgg atctcggtct ggatgataat ggttcgttgt
     6901 tgctggattt cggcccacgc cagttcaccg tcagctttga tgaaacctta aaaccgtttg
     6961 tgcgtgacgc ttccggcagt cgtctgaaag acctgcccaa accaaacaaa agcgatgatg
     7021 aattacaggc gaacaatgct gttaaccgct acaaattgct gaaaaaagat gcgcgtaccg
     7081 tcgccgccca gcaggtagca aggctggaat ccgccatgtg cctgcgtcgc cgctggtcac
     7141 cggaaaactt tcagctcttc ctggttgagc atccgctggt ccgccactta acccgccgtc
     7201 tgatttgggg cgtttatagc gccgaaaacc agctactggc ttgctttcgc gtagcggaag
     7261 ataacagcta cagcaccgct gacgatgatc ttttcaccct gccggaaggc gatatctcta
     7321 ttggtattcc tcacgttctg gaaatatcac cgacggatgc tgccgccttc gggcagcttt
     7381 ttgccgacta cgaactgtta ccaccgttcc gccagcttga tcgtaacagt tacgcgctga
     7441 cagaagccga gcgcaatgcc agcgaactga cacgctgggc aggcagaaaa tgcccgagtg
     7501 gtcgggtaat ggggctggca aataaaggct ggatgcgtgg tgaaccgcag gacggtggct
     7561 ggatcggctg gatgatcaaa cctctgggcc gctggtcgtt aatcatggaa atcgatgaag
     7621 gttttgcggt cggcatgtcg ccagccgaac tcagtgccga gcaactctta agcaagctgt
     7681 ggctatggga aggcaaagcg gaaagctatg gctgggggag taattcaaca caggaagcgc
     7741 agttctccgt actcgatgcc atcaccgcca gcgagctaat taacgatatt gaagccctgt
     7801 ttgaataagg aaagagcatg gacaaggaat taccgtggct ggcggataac gcccaactgg
     7861 aactgaaata taaaaaaggc aaaacaccac tcagccatcg caattggcca ggcgaaccgg
     7921 tacctgttat cactgaaaac atcattcaga cactgggtga tgaattgcta caaaaagcag
     7981 agaagaaaaa aaacattgtc tggcgttatg aaaacttttc actggagtgg cagtccgcca
     8041 tcacgcaggc catcaacttg atcggcgaac acaaaccctc aatcacggcc cggacaatgg
     8101 cggcgctagc ctgtatcgcg caaaatgata gccaacagtt gctcgacgaa atcgtccaac
     8161 acgaggggct ggaatatgca actgaggtgg tgattgcacg ccagtttatt gtacggtgtt
     8221 atgagagtga tcctctggta gtaacattgc aatatcagaa cgaggactat ggctatggtt
     8281 atcgctcaga aacctataac gaattcgatc tccgattgcg taagcatctc tctctggcag
     8341 aggaaagctg ctggcagcgt tgcgccgaca aactcattgc cgcactgcca ggaataacca
     8401 aagttcgccg cccttttatt gcgctgaacc tcccggaaaa acccgaaatc gccaacgagc
     8461 tggtaagcct tgaatgttcg caaactcatt ttcgctcaaa agagtggtta aaagttgttg
     8521 ctgatgaccc taaggcggtg aaagaactcg cacgttactg gagccaggat atatttagcg
     8581 atcgagaagc cagctacatg tcgcatgaaa accacttcgg ctacgcggcc tgcgctgccc
     8641 ttttgcgcga acaaggactg gcagccgttc cgcgcctcgc gatgtatgca cataaagaag
     8701 attgcggcag tctgctggta caaattaacc atccgcaagt catccgcacc ttgctactgg
     8761 tggctgataa aaacaaaccc agcctgcaac gtgtggctaa atacagtaaa aacttccccc
     8821 atgcgacgct cgccgcactg gcagaactgc tggcgttaaa agccccccct gcgcgccctg
     8881 gttatcccat catcgaagac caaaagctgc ctgcacagca aaaagcacgt gatgaatact
     8941 ggcatacgct gttacaaacg ctgatggcat cgcagccaca actggcagaa gaggtgatgc
     9001 cgtggttaag tactcaagcc cgggcagtgc tgaatagtta tttatcggca ccgcccaaaa
     9061 cggttattga tagcaccgat aacagccaaa tgcctgaaat cctcgtttca ccaccgtggc
     9121 gtagtaagaa aaaaatgaca gctccacgtc ttgatttggc accgctcgaa ttaaccccgc
     9181 aagtttactg gcaaccaggc gaacaagaga ggcttgcctc cactgagtct gcccgttatt
     9241 tcagcacgga atctcttgct gaacgcatgg aacaaaaaag tggacgagtt gtattacagg
     9301 aactgggttt tggggatgat gtatggctgt ttctgaatta tatactcccc ggaaaactgg
     9361 atgctgcacg caattcactc attgttcagt ggcattacta cccagggcgg gttgaagaga
     9421 tcatgaatgg ctggagctcc ccggaagctc aattagcaga acaggcgctt cgcagcggtc
     9481 acgtagaagt gttaattaac atatgggaaa atgacagcta ctcacgttat cgtccggaaa
     9541 agagtgtctg gaacctgtat ttattggcac agttgccgcg tgagatggct ttgcctttct
     9601 ggctacgtat caatgagaaa aagcatctgt ctgcgggtga ggactatttt ctcagtatct
     9661 tcggattgga tgctctacca ggtctgctgt tggctttttc acatcgtcca aaagaaacat
     9721 ttccgttaat tttaaatttt ggcgcaacag aactggccct gcccgttgcc cgcgtctggc
     9781 gacgtttcgc ggcgcagcgt gatctggccc gccagtggat tttacaatgg ccggaacata
     9841 cggctactgc acttatccct cttgtcttca ccaaacccag cgataatagc gaagctgcat
     9901 tacttgccct acgtttactt tacgaacagg gacatggtga attgctgcaa accgcggcaa
     9961 accgctggca gcgtacagat gtatggcctg ccctggagca tttacttaaa cagagtccaa
    10021 tagagattta cccggcacgc atgccaaaag cccctgattt ctggcatccg ctaatgtggt
    10081 ccaggccgcg cctaatcatt aatcatcaac ttattaccga tgacgctctg gaaattatcg
    10141 gcgaaatgct gcgctttacc caggggggac gttttcatag cgggctggaa caactgaaaa
    10201 cgttctgcca gccgcaaacg ctggcagctt ttgcctggga tctcttcact gcgtggcaac
    10261 aagctggtgc ccccgcaaaa gacaactggg catttctggc gttaagtctc tttggtgacg
    10321 aaagcacggc acgggatcta acgacacaga tcctcgcctg gccacaagaa ggcaaatcta
    10381 cccgtgctgt cagtggcctg aacatcctta ccctgatgaa taatgatatg gcgctgatac
    10441 agctgcatca tatatcgcaa cgggcgaaat cccgcccctt acgtgataac gcggcggaat
    10501 ttcttcaggt ggtcgcagaa aatcgcgggc taagccagga agagttagcg gacagattag
    10561 tcccaacact gggccttgat gatccgcagg cgttgagttt tgattttggt ccccggcagt
    10621 ttaccgttcg cttcgatgaa aaccttaacc cggttatctt tgatcagcaa aacgttcgcc
    10681 agaaaagcgt tccccggttg cgcaccgatg acgatcaact gaaagcgccc gaggcactgg
    10741 cccgattaaa agggctaaaa aaagatacga ctcaggtgag caaaaacctg ctcccccgtc
    10801 ttgaagctgc cctgcgtacc acccgacgct ggtcgctggc agattttcat tctctgtttg
    10861 ttaatcatcc ctttacccgt ctggttaccc agcgattaat atggggcgtg tatccggcaa
    10921 atgaaccgcg tcgtttactc aacgcctttc gtgtggccgc agagggggag ttctgcaatg
    10981 cgcaagatga gcccattgac ctgcctgcgg atgctctgat tggcattgcc cacccgttag
    11041 aaatgacaga aggaatgcgc agtgaatttg cacagctttt tgccgattac gaaattatgc
    11101 cgccttttcg ccagttggcg cggcgcacgg tgctgctcac acctgacgag tcaaccagta
    11161 acagcctgac tcgctgggaa ggtaaatccg ctaccgttgg gcagcttatg ggaatgcgat
    11221 acaaaggctg ggagtcaggc tatgaggacg catttgtcta tggcctgggc gagtaccggc
    11281 tggtccttaa gttttcaccc ggttttaacc actacaatgt tgatagcaaa gcgttaatga
    11341 gcttccgttc tcttcgagtg taccgtgaca ataaatccgt cacttttgcc gaacttgatg
    11401 tgtttgattt gagtgaggcg ttaagcgcac ctgacgtcat tttccattaa aaacacagcg
    11461 ggcagtgatg caactgcccg ttatcaacac gacttaccag cggggaaagc gatgatcgtg
    11521 caaaaagagc tggttgctat ttacgattat gaggtccctg tacctgaaga tccgttttcc
    11581 ttcagacttg agatccataa atgctctgaa ttatttacag gttccgtcta tcgactggag
    11641 cgattccggc tacgtccaac atttcatcaa cgtgatcgag aagatgctga cccgctaata
    11701 aatgatgcgt tgatttatat aagagatgag tgtattgatg agcggaaatt acgaggtgaa
    11761 tcacctgaaa ctgtaatagc aatttttaat cgtaaactac agaatatatt caaccaacta
    11821 atagaatatt actttattaa agggggaagc ggttttcccc cttagttgcg tttcaatttg
    11881 cccattactt caacaaatca atcaaccgta acagagccac taatagctcc agtacagcaa
    11941 tgagcacggt taacttttgt gtcagcgtgt ccatccgctc tccttattaa ggagcgcaac
    12001 accagcacct tacccttaca ctgcctgtcg acactggttt acgtttgttg agtcgccatg
    12061 ttgcactccg gatcagagca ctacctgcgg caccacccgt ccggtagcca ggacttgaaa
    12121 tgggttcgat ccggcgcaac ttcccaacag tcaggattat aaatctcatt atcttaattg
    12181 caacacggtc cagcggtggt tataattcgt tcgttgagat gccatgttgc actatcagta
    12241 acttaacatc aaagtagtaa ataaaagaac ctgcctcacc agcaggttct tttattttct
    12301 gcgatctact ttccagatat ttttcgccaa tagcccacca taaatattca catcccggcc
    12361 aaatagtacg ttgattataa aatcgatgaa tttataaata aaaatgagtt atatgatgaa
    12421 ttaataaaaa cagtaatcat tttattaaag ggggaaacgg tattccccct tagatgcgtt
    12481 tcaatttgcc cgttacttca acaaatcaat caaccgtaac agagccacta atagctccag
    12541 tacagcaatg agcacggtta acttttgtgt cagcgtgtcc atccgctctc cttattaagg
    12601 agcgcaacac cagcacctta cccttacact gcctgtcgat actggtttac gtatgttggt
    12661 acgaaacgtt gctctccgga tcagggcacc gcctgcggca ccacccgtcc agcagccagg
    12721 acttaaaatt ggtccgatcc ggcacaacct cccaacgggc aggattataa atctcattca
    12781 ctgaattgca atggagtgtg gatatggtta taatttattc gttggtacga aatgttgcac
    12841 aatcagcaag ttagcgtcat tgtagtaaat aaaagaacct gcctcaccag caggtttttt
    12901 tatttactgt gatctgcttt ccagatattt ttcgctcaaa caactaatgc ggcaaacatt
    12961 tattgcgcgt aaaatatcgt ttatttcatt aatacatttc agggatgaat atatgtcacc
    13021 gcagaataat catctgcagc gtccgcctgc cgctgtgtta tacgccgatg aactggcaaa
    13081 attaaaacaa aatgatcacg caccttgccc gcccggttgg cagttaagtt tgcctgcggc
    13141 ccgcgctttt atccttggcg atagcgcgca aaatattagc cgtaaagtcg tgatcagtcc
    13201 ctccgctgtc gaacgcatgt tagtcactct cgctaccggg cgcggtttga tgttggtggg
    13261 ggaacctggc accgcaaaat cgctcctgtc tgaattactg gcaaccgcta ttagcggcga
    13321 tgccgggtta accatccagg gcggcgcatc taccaccgaa gatcaaatca agtacggctg
    13381 gaactacgcc ctgcttatca accatggtcc gtcaacggaa gcgctggtcc ctgccccgct
    13441 ttaccaggga atgcgcgacg gcaaaattgt ccgttttgaa gagataacgc gtacaccgct
    13501 ggaagtacaa gactgtctgt taggtatgct ttctgacagg gtaatgacgg tgccggaact
    13561 caccggtgaa gccagtcagc tctatgcgcg agaaggcttt aatattattg ccactgccaa
    13621 tacccgcgat cgcggcgtca atgagatgag tgccgcgcta aagcgtcgct tcgattttga
    13681 aaccgtcttt ccgattatgg atttcgccca ggagctggaa ctggtcgcca gtgcttcggc
    13741 gcgtttgctg gcccatagcg gtattccgca taaagtgcct gacgcggtac tggagttact
    13801 ggtccgcacc ttccgcgatc tgcgtgccaa cggcgaaaag aaaacgtcaa tggataccct
    13861 gacggcaatt atgtccaccg ctgaagccgt gaacgtggcc cacgcagtgg gcgtccgcgc
    13921 ctggttttta gcgaatcgcg caggtgagcc agccgatctg gtggactgta ttgccggaac
    13981 catcgtcaaa gataacgaag aagatcgcgc tcgtctgcgc cgttacttcg aacagcgagt
    14041 cgccacacat aaagaagctc actggcaggc ttattatcaa gctcgccacc gcctgccgtg
    14101 aggaaagatg catgagcgag ccgttaattg tcggcatccg gcatcatagt ccggcctgcg
    14161 cccggctggt gaaatcgtta atcgaaagcc agcggccacg atacgtgttg attgaaggcc
    14221 cggctgattt taatgaccgg gtagacgaac tgtttttagc ccaccagctt ccggtagcta
    14281 tttacagtta ttgccagtat caggacggtg cagcccccgg gcgtggtgcc tggacgccat
    14341 atgctgaatt ttcgccggag tggcaggcgc tacaagccgc tcgtcgtatt caggcacaaa
    14401 cttacttcat cgatttgcct tgctgggcgc agagtgaaga agaggacgat tcgcctgata
    14461 cgcaagagga aagccagacc ttgctgctgc gtgccacccg catggataac agcgacaccc
    14521 tgtgggatca cttgttcgaa gatgaaagcc agcaaactgc attaccctct gcactggcgc
    14581 actattttgc acaactgcgg ggcgattccc ccggagatgc tctcaatcgt cagcgcgaag
    14641 actttatggc ccgctggatt ggttgggcga tgcagcaaaa caatggcgac gtgttagtcg
    14701 tctgcggtgg ctggcacgct tctgcactgg caaaaatgtg gcgcgaatgc ccgcaggaaa
    14761 ttaacacgcc ggaattgccc tcgctggcag atgccgttac aggttgttat ctcaccccct
    14821 acagtgaaaa gcgccttgat gtgctggcag gatacctttc cggtatgcct gccccggtct
    14881 ggcaaaactg gtgctggcgg tggggcttgc agcaggcagg tgaacaactg ctgaaaacgg
    14941 ttcttacccg tttgcgccag caccacttgc ctgcttcaac agcggatatg gctgccgctc
    15001 atctgcatgc gatggcactg gcacagttgc gtggtcatac actaccgtta cgcactgact
    15061 ggctggatgc catagcaggc tcgctgatta aagaagccct gaatgcgccg ttgccgtgga
    15121 gctatcgcgg cgttattcat cccgataccg atccgattct gctaacgttg atagacacat
    15181 tagcgggtga cggattcggt aaacttgccc cctctacgcc acagccgcct ctgccaaaag
    15241 atgtcacctg cgaactggaa cgtacaggaa tctcttttcc ggcggagctt actttaaatc
    15301 gctttacccc cgatggactg gcgcaaagtc aggtgttaca tcggctggta atactggaga
    15361 tcccaggggt tgtgcgccag cagggaagta cactgacact tgcaggcaac ggtgaagaac
    15421 gctggaaatt aacccgcccg cttagccaac atgcggcatt aattgaggcc gcctgctttg
    15481 gtgccacact ccaggaagcc gcacgcaata aattagaagc cgatatgctg gacgcgggtg
    15541 gaatcggcag tatcaccaca tgtcttagcc aggcggcgtt agcgggtctg gcgtccttca
    15601 atcaacaatt actggagcaa ctcacattat taatcgccca ggaaaatcag tttgccgaaa
    15661 tgggccaggc gctggaagtg ctatatgcct tatggcggct ggatgaaatt agcggtatgc
    15721 agggcgcgca gatattacaa acgacattat gcgcggccat cgatcgcacg ctgtggctat
    15781 gtgaatccaa cggcagaccg gatgaaaagg agtttcacgc tcacctgcat agctggcaag
    15841 cgctttgcca tatcctgcgc gatctacata gcggcgttaa tttacccggc gtttctcttt
    15901 ctgcggcggt agccttactg gagcgacgca gtcaggcgat tcatgccccg gcgctggatc
    15961 gcggcgcggc acttggcgca ctgatgcgtc tggaacatcc caacgccagt gccgaagcgg
    16021 cgctgacaat gctggtgcaa ttatccccga cacaatccgg cgaggcgctg cacggtttgc
    16081 tggcattagc ccgtcatcaa ctggcctgtc agccggtatt tatcgccggt ttcagcagtc
    16141 atttaaatca actgagtgat gccgatttta tcaatgccct gcccgattta cgcgcggcga
    16201 tggcctggct accaccacga gaacgcggga cgctggcgca tcaggtgctt gagcattatc
    16261 aactggtgca acttcccgtt tcggcactgc aaatgccgtt gcattgtcca ccacaagaca
    16321 ttgcacatca tcaacaactc gaacagcagg cactggcatc gctgcaacac tggggagttt
    16381 tccatgtctg aactgaacga tcttctgacc acccgtgagc tacaacgctg gcgattaatt
    16441 cttggcgaag cggcagaaac gacgctttgt gggctggatg acaacgcccg gcagatagac
    16501 cacgcgctgg agtggctgta tgggcgcgat cctgaacggc tccagcgtgg tgaacgctcc
    16561 ggtggattag gtgactcaaa tctcaccacc cctgagtgga tcaacagtat tcacacgctg
    16621 tttccgcaac gggtgatcga gcgactggaa agcgatgccg tactgcgcta cggcattgaa
    16681 gatgtggtga cgaatctcga cgtgctggaa cgtatgcagc cttctgaaag cctgctacgc
    16741 gccgttttgc acaccaaaca tctgatgaat cccgaagtgc tggctgccgc ccgccgaata
    16801 gtgcgccagg ttgttgaaga aattatggcg cgactggcaa aggaagttcg tcaggctttt
    16861 tctggtgtcc gcgatcgccg ccgtcgctca tttattccac tggcgcgtaa tttcgatttc
    16921 aaaagtacgc tgcgtgccaa cctgcaacac tggcatccgc aacacggcaa gttgtatatc
    16981 gaatcccccc gctttaacag ccgcattaag cgccatagcg atcaatggca actggtctta
    17041 ctggttgatc aaagcggatc gatggttgac tcggtgatcc actcagcggt gatggcggcc
    17101 tgtttatggc agttacccgg cattcgtacc catctggtgg cgtttgacac cagcgtggtt
    17161 gatctcacgg cagacgttgc cgatccggta gagttattaa tgaaagtaca actgggcggc
    17221 gggaccaata tcgccagtgc cgtggagtat ggtcggcaac ttattgaaca acctgcaaaa
    17281 agtgtcatta tcctcgtgag cgatttctat gaaggtggtt catcatcatt gctgacgcat
    17341 caggtgaaaa agtgtgtcca gagcggcatc aaagtgctgg gactggcggc gctcgatagc
    17401 accgcaacac cttgctatga ccgcgatatg gcccaggcac tggttaatgt cggtgcacaa
    17461 atagccgcca tgacgccggg cgaactggcg acctggcttg cggagaatct tcagtcatga
    17521 attcactacg tccggaatta ttagaactga caccacaggc actgacggcg ttaagcaatg
    17581 ccggttttgt taagcgcagc cttaaagaac tggaaaatgg caacgtcccg gaaatcagtc
    17641 atgagaacgg cgctttaatc gccaccttca gtgacggtgt ccgcacccag ctggcgaacg
    17701 gccaggcact gaaagaggct cagtgcagct gcggggccag cggcatgtgc cgccatcgcg
    17761 tgatgctggt tttaagttat cagcgacttt gcgccaccac ccagcctacg gaaaaagaag
    17821 aagagtggga tccggcaatc tggctggaag aactggctac cctgcccgat gccacccgca
    17881 aacgcgcaca ggcgctggtc gctaaaggca tcaccattga gttgttctgt acgccgggcg
    17941 aaattccctc tgcccgctta ccgatgagcg atgtacgttt ttattcccgc agcagcattc
    18001 gtttcgctcg ctgtgattgt attgacggca cactttgcga acatgtcgta ctggctgtgc
    18061 aggccttcgt cgaggccaaa actcagcaag cggaatttac tcatttaatc tggcagatgc
    18121 gcagcgaaca cgtcacatca tctgacgatc cgtttgccag cgaagagggc aacgcgtgtc
    18181 gtcaatatgt tcagcaatta agtcaggcat tatggctggg cggcatcagc cagccgccaa
    18241 tccattacga ggccgctttc agccgcgcac agcaggcggc ggaacgctgc aactggcgat
    18301 gggtgagtga atcgctacgg caactgcgcg ccagcgttga tgccttccac gcccgcgcca
    18361 gccactatca tgccggaaaa tgcttgtgcc agcttgcggc gttaaccagt cgcttgaaca
    18421 gtgcacaaga gatagcgcga cgtgacagtg tcggtgaagt accgcccatg ccgtggcgca
    18481 cggtcgttgg ctctggcatt gccggagaag caaagcttga tcatctgcgg ctggtgtctt
    18541 taggtatgcg ttgctggcag gatattgagc attatggttt acgcatctgg tttaccgatc
    18601 ccgacaccgg cagtattttg catctttcgc gtagttggcc gagaagcgaa caggaaaact
    18661 caccggcagc gacgcgtcgg ctgtttagtt ttcaggctgg cgcactggcg ggcgggcaaa
    18721 ttgtttcaca agcagcaaaa cgcagtgccg atggcgagct gctgttagct acccgcaacc
    18781 gcttaagcag cgttgtgccg ctgtcgcctg atgcctggca aatgttgagc gcgccgttac
    18841 gccagccggg cattgtggct ttgcgggaat atttacgcca gcgtcccccc gcctgcatac
    18901 ggcctcttaa tcaggtcgat aacttattta ttctgccggt cgctgagtgt atttcgctcg
    18961 gttgggatag cagccgccag acgctggatg cgcaggtaat cagcggcgaa ggtgaagata
    19021 atctgctgac gttatcactg ccagcctcag ccagcgcgcc ttatgccgtt gaacgcatgg
    19081 cggcgctttt gcaacaaaca gacgaccccg tgtgtctggt ttctggcttt gtcagctttg
    19141 tagaggggca attgacactg gaaccacagg taatgatgac aaagactcgt gcctgggcgc
    19201 tggacgcaga aactgcgcct gtggtggcat cactaccttc tgccagcgtt ctgcctgtgc
    19261 cgtctactgc tcatcagttg ctgatgcgct gccaggcgtt acttattcaa ctgctccata
    19321 acggctggcg ctatcaggaa catagtgcta ttggtcaggc agagttgctg gcgaatgacc
    19381 tcaccgcggt gggtttttat cggctggcac atgtgttggg acaatttcgt aatacagaaa
    19441 gcgaggcacg ggtagaagca atgaataacg gtgttttgct gtgcgaacaa ttattcccca
    19501 tgcttcagca acaaggatga aatagtgctt tttactaaga gttctactcc agttccggac
    19561 tgctcacgcc acggtattag gcatatccta tatagcccct ggtgagagtc accagttcct
    19621 tgattaaata aaatggagtt ttacatgaag gctttcaata agctgttttc cctcgttgtt
    19681 gcatctgttc tggttttctc tcttgctggc tgcggtgaca aagaagaatc gaaaaaattc
    19741 agcgccaatc tgaacggtac tgaaattgcc attacctatg tctacaaagg tgacaaggtg
    19801 cttaagcaat cttctgaaac caaaattcaa tttgcctcca ttggtgcaac caccaaagaa
    19861 gacgctgcca ggacacttga gccgttaagc gccaaataca aaaacatcgc gggtgttgaa
    19921 gaaaaattaa cctataccga tacttacgcg caggaaaacg tgactatcga tatggaaaaa
    19981 gtggatttca aagccctgca gggtatttca ggaatcaacg tttctgctga agatgccaaa
    20041 aaaggtatca ctatggcgca aatggaactg gtgatgaaag ccgctggttt taaagaagtg
    20101 aaataatctg tcggcggcca tgtttttgca tggccgccat acccgtctta gcttttcttc
    20161 acatgctggc gcgctgccag tccacgcaga aaataacgta aaaattgatc gccgcattcg
    20221 cggaagtttt tatgatccgg tgcgcgcatc atcgctgtaa tttccggcat cgaaacgcgg
    20281 aactgctgtt cggtgaggat cgccagaatg tcatcggttt tcagcgaaaa cgcgatgcgt
    20341 aattttttca gcacgatgtt gttattaatg cgacgttccg gctccagtgc cggagcagac
    20401 tcatccttgc cgcgtttttc ataaatcagg ccattgagga atgacgacaa aacaatgtcc
    20461 ggacaacgct gaaaaccctc ttcgtcttct ttacgtagcc agacggcgat ctgttccgcg
    20521 gtggcttcga cattacccag cgccagaata cgcaccaggt cattattatt ggctttcaaa
    20581 atgtagcgca cgctgcgcag aatatcgtta cttagcatga ggccttcagg tgttgatgag
    20641 gcaaaaagcc attttagcag tcttttacag gccaatcgcc tcttttaagc ttttcagata
    20701 acggcggctg accggcacgg ttaagccatt acgcaaaatc aactcggcct ggccgttatc
    20761 ttccagacga atctcctgta aatgcgcgag gttaaccaga tactgacgat ggcagcgcag
    20821 tagtggtgta cgactttcca gggtacgtaa tgtcaattcg gtaaagccct ctttcccttc
    20881 gtggctggta acgtagacac cgctcatccg actgctgaca aatgccacat ctttcatttg
    20941 cagtaaataa atccgactat gccccgtaca agggataaat ttcagcgcct gttgattttc
    21001 cggtaacagc gaaacatcct gcttactgcg ttcctgacgc aaacgcgcca gcgttttctc
    21061 cagtcgcgct tcatcaattg gcttcagcag atagtcaaag gcatgttctt caaaggcttt
    21121 aatagcgtat tcgtcaaacg cggtgagaaa aacgatatac ggtcgatgtt cggggtcgag
    21181 catccccacc atttccagac cactgatgcg cggcatctgg atatcgagaa acagcacatc
    21241 cgggcgcagt ttatgcaccg cgccgatccc ttctacggcg tttgaacact ctccaacgat
    21301 ttcaatatcg ctctgctcct gcaaaaatac gcgcaggttc tcccgtgcaa gcggttcatc
    21361 atcgacaatt aagactttaa tcatgcctcg tccctccatg gtagtcgtaa cgttattcgg
    21421 gtgtaacaat caggctcaca ggcgacgctt atcccatagt catcgccaaa ccgttcacgt
    21481 aaacgcttat ccaccagatt catccccagc ccactggcat tggttaccgg ttgatacaaa
    21541 ccggcattgt cttcgatctc cagcatcaaa tgttgcccct cacgtcgggc gctgatcgcc
    21601 actcgccctg tatccagcag ttgtgatgtc ccatgtttaa tggcgttttc cactatcggt
    21661 tgcagggtaa acgcgggcaa ttgctgctgg gataattctt gcggaatagc aatgttgacc
    21721 tgcaaccgcg actggaagcg cgccttttca atttgcagat aagcgttcac atgttcaatt
    21781 tcgtctgcga gagtaacaaa ctccgaaggc cgctttaagt ttttgcggaa aaaagtggaa
    21841 agatactgga ccagctggct ggcctgttcg ctgtcgcggc ggatcaccgc tttaatggtg
    21901 ttaagcgcat taaacaaaaa atgggggttc acctgggcgt gaagcagttt gatctctgac
    21961 tgggtgagca tcgctttttg ccgctcatat tgcccggcga ggatctgcgc cgaaagcagt
    22021 tgcgcaatcc cctcgcccaa cgtgcggttg attgaactga ataaacggtt tttggcttca
    22081 tacaatttga tggtgcccat cacccgctga ttttcgccac gcaacggaat gaccagcgtc
    22141 gaccccagtt tgcattgcgg atgcaaagag caacgataag gtacttcgtt gccatcagcg
    22201 tagaccactt caccggtttc aatcgctttt aaggtgtaag tcgaagaaat cggtttgccg
    22261 ggtaaatggt ggtcgtcacc aattccggta aaggccagca atttctctcg atcggtaatc
    22321 gcgactgcac caatatccag ctcctgatac agcacctgtg ccactttcat gctgttcact
    22381 tcgttaaacc cctggcgcaa aatgccttcc gtcgaggcag ccacttttag cgcagtggca
    22441 gaaaaagccg aagtgtattt ttcaaacatc gcgcgtttat cgagcaatat acgcataaac
    22501 agcgccgcgc cgacggtatt ggtaaccatc attggcgcag caatattact caccagacgc
    22561 accgcatctt cataaggtcg ggcgatcgcc aggatgatca acatttgcac catttcagcg
    22621 acgaacgtga cggcaccggc ggtaatgggg ttaaagactt tatcagtgcg cccgcgacgg
    22681 atcaggatgc tgtgtaccag gccgccgagc aacccttcaa cgatggtcga gatcatgcaa
    22741 cttagcgcgg tcatgccccc catcgaatat cgatgtaacc cgccggtcag accaaccagc
    22801 ccaccgacga ccggaccgcc gagtaagccg cccattaccg cgcctatcgc acgggtattg
    22861 gcaatagaat cgtcaatatg cagcccaaac caggtgccca tgatgcagaa gatggaaaag
    22921 acgatgtagc agagaaattt atgcggcaga cgaaccgtga cctgcattaa cggtatgaat
    22981 aatggcgttt tactcattaa ccacgcaatg actaaaaaaa cgcacatctg ctgaagcagc
    23041 agcaacacca gattaaaatc gtacataccc gcaaaccaca cttcccttta aaacgcgtaa
    23101 catacattgc ctgcgtttaa ctttctttga actcttgcag aaaaatgaga attcgtgagt
    23161 acgatcactc aaaatcgcct ggcaaaaata aaatcaccct atagatgcac aaaaaacggg
    23221 caaaactacc tggttcgcaa aactgcgtct aaagttaaac cgggacctcg cgagcaaggg
    23281 tgagacgatg gcgctttaca caattggtga agtggcgttg ctttgtgata ttaaccctgt
    23341 cacgttacgc gcgtggcaga ggcgttacgg attgctgaaa ccgcaacgga cagacggcgg
    23401 tcatcggctg ttcaatgatg ccgatattga ccggatccgc gagatcaaac gctggatcga
    23461 caacggcgtg caggtcagca aagttaaaat gctgctcagt aatgaaaatg ttgatgtgca
    23521 gaacggctgg cgcgatcagc aagaaacatt actgacttac ctgcaaagcg gcaatctgca
    23581 tagcctgcga acgtggatca aagagcgcgg tcaggattac cccgcccaga cactcaccac
    23641 gcatctgttt attcctctgc gccgacgact tcagtgccaa caaccgactc tccaggcgct
    23701 gctggcgatc ctcgacggcg tactgatcaa ctacatcgcc atttgcctgg cttcggcacg
    23761 taaaaaacag ggtaaagatg cgctggtggt tggctggaat attcacgata ccacccgtct
    23821 gtggctggag ggctggattg ccagtcaaca aggatggcgc attgatgtcc tcgcccactc
    23881 gctcaatcaa ctacgccctg aactgttcga aggccgtaca ttgctggtgt ggtgcggtga
    23941 aaatcgaacc tccgcccaac agcagcaact caccagttgg caggaacaag gccatgatat
    24001 tttcccactc ggcatttaat gattcgttaa caaatgcgct ttactgtaca atcctttcgt
    24061 taacataagg agtgcattat gcgcatagct aaaattgggg tcatcgccct gttcctgttt
    24121 atggcgttag gcggaattgg tggcgtcatg ctcgcaggtt atacctttat tttgcgtgct
    24181 ggctaagcgc ctgcaccagc ctttcaaaca ggcggtctgc aatgatcgcc gccagtgcca
    24241 ccagtaacgc cccctggatc acatacgcgg tattaaatcc gctaagcccg atgatgatgg
    24301 gcgtacccag cgtgctggcc cctaccgttg aggcgatcgt cgccgtacca atgttgataa
    24361 tcaccgaagt tcgcacaccc gccagaatca ccggagccgc cagcggtagc tcgaccttac
    24421 gcagtcgctg acctcgactc attcccatac ttttcgcaac ttctgtcacg ctggcatcaa
    24481 tcgctcccag cccggcaagt gtcgcctgca ggacgggcag cacaccataa aggatcaagg
    24541 cgataatcgc gggttgcaga ccaaacccaa tcaccggaac ggcaatcgcc agcaccgcga
    24601 cgggcggaaa agtctgtcca acggcggcaa tagtttccac cagtgggcga aattccgcgc
    24661 cccacgggcg agtaacaata attcctgcac cagtgccaat gatcaccgca aacaaactcg
    24721 aaattcccac cagccagaaa tgagccagtg ccagagcagc aaaactttct tgctgataaa
    24781 cggggcgtgg cagttgtggg aacaaggcag caaacagcgg ctggctgtaa ggcagccaga
    24841 aaatcagtgc cacaaacaaa gcaatgagcc agaacagcgg atcgcgcaac atcttcatac
    24901 gcttacgcct ccaccagcag atcctgaaaa tgcagcgtgc cgcaaggctc gccctgcgtg
    24961 ttcaccaccg gcagcacttc acatccccgc gcgacaaaca gggagagcgc atcgcgtagc
    25021 gtcatctctt ctgccagcgc ctcaccttct gcccgttctt cgcgacgcac gtaatccgcc
    25081 acgctacgta acgaaagcag gcggacaccc agttcactat gcccaaaaaa ctggcggaca
    25141 aaatcattcg ccggacgagt cagcatcgtc agcggattgc cctgctgcac cacttcaccg
    25201 tgatccatca acaccagatg ttctgccagt cgtagcgcct catcaatatc atgagtgacc
    25261 agcacaatgg tacgccccag caaacggtgt atgcgcgtca tctcttgttg caacgcgccg
    25321 cgcgttaccg ggtccagcgc gccaaaaggt tcatccatca gtaagacttg cggatcggca
    25381 gccagtgcgc gcgccactcc cacacgttgc tgctgaccac cggaaagctg atgcggataa
    25441 cgctcacgca aatttggctc cagccccagt agcgccatta attcgtcgat acgatcgtcg
    25501 atccgcgccc gtgaccattt ttgtaattgc ggcacggtgg caatattttg cgccacgctc
    25561 cagtggggaa acaggccaat agattgaatg gcatagccca tccggcggcg caactccagc
    25621 actggcagcg agcgaatttc ttctccggca aagcggatca cgccgctgtc atgctccacc
    25681 aggcggttaa tcattttcag ggtggtggat ttaccggagc ctgatgtacc aatcagcacc
    25741 gaaaaactcc cctcctgaaa attgagattg agatcgttaa cggctttttg tgcgccgaac
    25801 agtttgctga catggctaaa ttcaatcatt acgtttcacc ttcagcagtg cgataagcaa
    25861 atcgaacagc gcatcgatca gcaccgccag aacaattacc gggatcaccc ccagcaacac
    25921 taaatcaatg gcactgctta gcagcccctg gaaaaccagc gcaccaaaac cgcctgcgcc
    25981 gattaacgcc gcaatcaccg ccatgccgac ggtttgcacc atcaccaccc gcaggctacg
    26041 cagaaatacc ggtaacgcca gcggtaactg aacatgcagg aatcgctgcg ccccgctcat
    26101 gcccatcgct ctggcgctct ccagcacatc gcgcgggatc tggttcaagc cgactaccac
    26161 gccgcgcacc agcggcagca aggcatagag caccagcgca atcagtgcgg gtgtcattcc
    26221 ggttcctgat atgccgagct tacccagcca cggaaaggcc gtaaccagcg cggcaagcgg
    26281 cgcaatcaac aggccaaaga gcgccaccga aggcacggtc tgaatgacat tgagtagcga
    26341 aaaaatcgct ccctgccgcg cagtggaaaa gtagcaccag acgcccaacg gcacaccaat
    26401 cactaacgca ggcagcaccg caccaaacag caacgtcaga tgttgtgcca gcgcgtcgtc
    26461 aaacacatcc tgacggttgg cgtattcttt cattagcgag agatcgttaa gcgtgccgga
    26521 gtacagcaac cacagcggaa taatggcaat ctgcatatgc aacaaccagc gccacagcag
    26581 atgcgtggag attcggcgga tggcatcgct acaggccagc aatgccagcg ccgcagccag
    26641 ccagaaacca ctgccgaggc tggtacgcgc cagcgcactg ccattttgtg ccagttgtgt
    26701 cgccgccttt ccagctcccc acaccagcaa tacgaagacg aattgcgcca gaatgagtgc
    26761 acaaatgctg ccttttttac cgggaataac acaggcggtc agccaggcgc aaccaacgcc
    26821 caccagcatc cagatcgttt gcggccacag ctgccagaga tgacgcccct cacccgaaac
    26881 taaacgatta ggcgcgtaac tgataaacgg cagcgccgct gcgattgccg tcagcaacag
    26941 cagcagcgcc agaacaggat taatacgtag ataagtcacg ggaaattact tcgtccaccc
    27001 tttttgtttc aggtagtcgg cagccacttt tttggcatcc agtccttcaa ctgcaatgct
    27061 ggcattcagt tgctgcaatg tttttgcatc gaggctggcg aagactggct gtagccactg
    27121 tgccatttgc ggatactcct tcaacaccga ttcacgaacc actggtgcag gcgcgtagat
    27181 aggttgcacg ccttgcggat cgcttaaggt ttgcaacccc agcgctgcga ccggaccgtc
    27241 agtgccgtaa gccattgcgg cattaacgcc agaagtttgc tgggcagcgg ctttgatcgt
    27301 taccgccgtg tcgccaccag ccagtgacag caactgatcc tgatcgagtt taaagtcgta
    27361 ggctttttca aaagcgggta aggcatcggc gcgttcaata aattctgccg aggccgccag
    27421 tttgaaggtg ccgccctctt tcagataacg actcaggtcg gcaagcgaag tgagtttgtt
    27481 tttctctgcc acatcctgac gcacggcgat agtccaggtg ttatttgcag gcgcgggcgt
    27541 cagccagatt aacttgtttt gctctgcatc gagttttttg actttctcgt agccttgccc
    27601 ggcatttttc catgccgcat cgttttcatc tttaaagaaa aacgcgccat tgccggtata
    27661 ttccggatag atatccagtt caccggaagt aatcgccccg cgcaccacgg gagtcgttcc
    27721 cagttgcact ttatttaccg taggaacacc gtgactttcc agcacctgca aaatgatatt
    27781 gccgagcagc gcgccttcgg tatcgatttt tgaaccgacc ttaacggggg aagccgcctg
    27841 tagcggcagg ctcacggctg ccaacaaaac caatgaacct gcccagagct ttgagagtgg
    27901 catgatgctt tcctcattct tttactgttg ttttcagcga attaagagaa aagcatagtt
    27961 gataatgaca gggttagcct gtctgggcgg attcagttgc agaatcagat aaatacttaa
    28021 agggcgcaga cgtcgcgccc ttactaaagc attacagcaa ctcaaactcg cctttcttaa
    28081 cgcgtgcgga atcagtgccg ataaagacat tgaacttgcc aggctcggcg tcatatttca
    28141 tctgttgatt ccagaacttc agcgcctcga tatcaatcgg gaagctgacg gtctgggttt
    28201 cgcctggttt cagggtgatt ttctcaaagc ctttcagctg cttcaccgga cgactcatgg
    28261 aagccgtcac atcctgcaag tacatctgca ctaccgtcgc cccttcgcgc ttaccggtgt
    28321 tcgtcacctg cacactggcg gtcactttgc cgtcacgctt catggtcggc gcggaaagtt
    28381 tcacatcaga gacggtgaaa gtggtgtagc tcagaccata gccgaacgga tacagcgccc
    28441 cgttagcttc atcaaaataa cgcgacgtgt atttgttcgg cttatcggcg ttatacgggc
    28501 gaccggtatt cagatggctg tagtacaccg ggatctgccc gacagaacgc gggaaggaca
    28561 tcggcagctt gccggacggg ttgtaatcac caaacaatac atcggcaatt gcattaccgc
    28621 cttcagtccc cgcaaaccag gtttccagaa tcgcatccgc ctgctgatct tctttcacca
    28681 gcgccagcgg acgcccgttc atcagcacca gcaccagcgg tttagcggtg gctttcagcg
    28741 ccgcaatcaa gtcacgttgg ctttgcggaa tagtgatatc ggtacggctg gaagcctcgt
    28801 gcgccatccc ctgtgcttca ccgactacag ccaccaccac atcagattgt ttcgcggtct
    28861 gcaccgcttc atcaatcatc tcttgcggcg aacgcggatc gactttgacc gcttcttcat
    28921 actgattcag gaaatcgata atgcctttgt cactggtaac gttcgcccct ttggcataca
    28981 gcactttacc gttttcaccg acggcgtttt taatccctgt tagcacagta acagattgat
    29041 cggcgacacc ggctgcggac cagcttccca tcacgtcacg cttgctgtct gccagcgggc
    29101 caaccaccgc aatggtggct gattttttca gcggtaacgt ttcgagacgg tttttcagca
    29161 acaccaggct ttcgcgtgcc acttcacgcg cttctttgcg gtgcaggcgg ctttcggcat
    29221 tggtatccac cgggtcagac tctttcggcc ccaaatggct gtatgggtcg ttaaacaacc
    29281 ccatatcata tttaacgttc agtacatgac gggcagcgtc atccagctct tccatcgtca
    29341 ctttgccgga tttgatcaac ccaggcagat acttcgagta atactcgtcg ctcatactca
    29401 tgttgatgcc ggatttcagc gccacgcgca ccgcatcttc cgggtctgcc gccgtgccat
    29461 gtttaatcag ctctttgatt gcgccgtgat cggaaacggt gatgccttta aagccccact
    29521 ggtcgcgcag aacatctttc agcagccagg agtcggaggt ggctggcgta ccgttcagcg
    29581 agttcagcgc caccatcacc gcgccgctgc ctgcgtccag ccccgctttg tacggcggca
    29641 tataatcatt aaacaggcgc tgcggactca tatcgacggt gttgtactct ttaccgcctt
    29701 ctaccgcgcc gtatgcggca aagtgtttga cgctggtcat caccgagtaa cgatctgccg
    29761 ggcttttacc ctgcatcgct tccaccatgg ttttacccat tgttgaagtg agatacgtat
    29821 cttcgccaaa accttcggaa gcacgtcccc agcgcggatc gcgcgagaca tcgaccatcg
    29881 gtgcccaggt catattcagg ccatcatctg ccgcttcata agcagagaca cgtccgaccg
    29941 ttttcaccgc atcgaggtta aaagacgagg ccagaccgag gctaatcggg aacaccgtgc
    30001 gctgaccgtg cagcacgtcg taagcaaaga aaagaggaat tttcaggcgg cttaattcca
    30061 tcacctgatc ctgcatggcg cggatatcct gacgggttac ggtgttgaaa atcgccccaa
    30121 cctgcccgtc tttgatcatc tcgcggatcg cctctttcgg attatccggg ccgacgctga
    30181 ttaagcgcag ctgaccaatt ttctcatcaa ccgtcatttt cttaagcagt tcggtgacga
    30241 acgcatcccg cgcttcaggc gttaagggat ggttaccgaa taaatcatcc gccagtgctg
    30301 gctgcagggc cagacttacc gcgattccta ctgaacatag ccatttcata tggatttttc
    30361 ttcctttgtt gcccgacgtg gcagcgaaaa tggtgcaaaa accgtagttt gccataagca
    30421 tgatggagag agaaaaagaa tgctcagttt attgtctgaa ttttcaaaat attcactcgc
    30481 tgaattgtca tacaaggcgc tattctagtt tgtgatattt tttcgccacc acaaggagtg
    30541 gaaaatgtct tccatgacaa caactgataa taaagccttt ttgaatgaac ttgcccgtct
    30601 ggtcggtcat tcacacctgc tcaccgatcc cgcaaaaacg gcccgctatc gcaagggctt
    30661 ccgttctggt cagggcgacg cactggctgt cgttttccct ggctcactac tggaattgtg
    30721 gcgggtgctg aaagcctgcg tcaccgccga caaaattatt ctgatgcagg ctgccaatac
    30781 aggcctgacc gaaggatcga cgccaaacgg taacgactat gatcgcgata tcgtgatcat
    30841 cagcaccctg cgtctcgaca agctgcacgt tctcggcaag ggcgaacagg tgctggccta
    30901 tccgggcacc acgctttatt cgctggaaaa agccctcaaa ccgctgggac gcgaaccgca
    30961 ctcggtgatt ggatcatcgt gtataggcgc atcggtcatc ggcggtattt gtaacaactc
    31021 cggcggctcg ctggtgcaac gtggcccggc gtataccgaa atgtcgttat tcgcgcgtat
    31081 aaatgaagat ggcaagctga cgctggttaa ccatctgggg attgatctgg gcgaaacgcc
    31141 ggaacagatc cttagcaagc tggatgacga tcgcatcaaa gatgacgatg tgcgccacga
    31201 tggtcgtcac gcccacgatt atgactatgt ccaccgcgtt cgtgatattg aagccgacac
    31261 gcccgcacgt tataacgccg atccggatcg gttatttgaa tcttctggtt gcgccgggaa
    31321 gctggccgtc tttgcggtac gtcttgatac cttcgaagcg gaaaaaaatc agcaggtgtt
    31381 ttatatcggc accaaccagc cggaagtact gaccgaaatc cgccgtcata ttctggctaa
    31441 tttcgaaaat ctgccggttg ccggggaata tatgcaccgg gatatctacg atatagcgga
    31501 aaaatacggc aaagacacct tcctgatgat tgataagtta ggcaccgaca agatgccgtt
    31561 cttctttaat ctcaaggggc gcaccgatgc gatgctggag aaagtgaaat tcttccgtcc
    31621 gcattttacc gaccgtgcga tgcaaaaatt cggtcacctg ttccccagcc atttaccgcc
    31681 gcgcatgaaa aactggcgcg ataaatacga acatcatctg ctgttaaaaa tggcgggcga
    31741 tggcgtcggt gaagccaaat cgtggctggt ggattatttc aaacaggccg aaggcgattt
    31801 ctttgtctgt acgccggagg aaggcagcaa agcgttttta caccgtttcg ccgctgcggg
    31861 cgcagcaatt cgttatcagg cggtgcattc cgatgaagtc gaagacattc tggcactgga
    31921 tatcgctctg cggcgtaacg acaccgagtg gtatgagtat ttaccgccgg agatcgacag
    31981 ccagctggtg cacaagctct attacggcca ttttatgtgc tatgtcttcc atcaggatta
    32041 catcgttaaa aaaggcgtgg atgtgcatgc gttgaaagaa cagatgctgg aactgctaca
    32101 gcagcgcggc gcgcaatacc ctgccgagca taacgtcggt catttgtata aagcaccgga
    32161 aacgttgcag aagttttatc gcgagaacga tccgaccaac agtatgaatc cgggggttgg
    32221 taaaaccagt aagcggaaaa actggcagga agtggagtaa aaattacgga tggcagagta
    32281 tcgccatccg tcattcattt aatcgttctg tgccgtctgc cccgccgccg ccatttgggc
    32341 ggctttttgt tttttatagc tcaacgctgc tgccggaaca ggcatcactt taccggtctc
    32401 aatccaggta cgcaggcggc tggcatcggc aaaatgggta tatttgccaa acgcgtccat
    32461 cactaccagc gccaccggtt tattattgat aaccgtacgc atcaccagac aatggcccgc
    32521 cgcattggta aagcccgttt tggttaactg aatattccag ttatcgcgat acaccagatg
    32581 gttagtattg cggaacggca gcgtatacgt cgggttagag aaggttgcca tatcttccct
    32641 ggtagtactt aactgaccaa tcaacggata ttgtttgctg gcaatgagca gtttggttaa
    32701 gtcacgggca gttgaaacgt tatgcaccga caatccggtc ggttcaacaa agcgcgtgtt
    32761 gttcattccg agcgatttcg ctttcgcatt catcgcctta ataaaggctt tgtaaccacc
    32821 gggataatgg tgcgcaaggc ttgccgccgc gcggttttct gaagacatca gcgccagcaa
    32881 cagcatatct ttacggctga tttcgctgtt cagtcgtacg cgcgaataga cccctttcat
    32941 ctccggcgtc tggctgatat ccacttttag tttttcatcc agcggcagtc gtgcatccag
    33001 cacaaccatc gcggtcatta atttgctgat agacgcaatc ggacgcacca gatccgggtg
    33061 gttcgaatag atcactttgt tggtattcag atcaacaatc atcgcgctac cggaggcaat
    33121 ttccggttgt gaagcggtgg tagcggctgc cgttttcgca acggcctgcg gtgccaaagg
    33181 cacagccagc atcagggcca ggctaaataa agaaactcga aatttcggca tgatgagcat
    33241 tcagatagtg gttcacgcgc acgggttgcg caccgccgga gtaaggattt actgaggcta
    33301 gcgacgccat cataacgagc aaaaagtgcg atcgtcaaag aagaatcgtg aggaaatgct
    33361 gcattgctga catttacgcc agcaatgcag cgcaaaagaa ctttctagaa caaacgataa
    33421 ccgtagcccc atagtataac ggtaagggcg agcagcactt ccagcaccag cacgcctatc
    33481 gccagtgtcg aactggagaa gctaagtcct tcctctttgt tgatattcag gaagctcgga
    33541 ataccgagat acagcaggta gccggtgtaa aacagcgcca ccgtgccgac cagcgcacac
    33601 aaccagacca gcgggtaaag cgccaccaga ccgcttaaaa acagcggagt tgcaacgtag
    33661 cccgcgaaga ccatgcagtg cgcaagtgac ggacgctgcg gataattacg cgccatccac
    33721 cagatgaccc gccccatcac cgcaacccca gccagcataa cgccgtaaaa caagacagcc
    33781 agcgccagtc cggtaaacca ggataacttc aggatagtgc catcgccaaa attccagcca
    33841 atctgtgtag tgccaatgaa ggcgcaaatc accggaatcg ccgccatcag caaaacgtgg
    33901 tgggtgtaat ggtgagaaat cgtttcgttt tcgcgattaa tcacctgcat ttcacgatcg
    33961 ggatgggaaa acagtcccca gacatggctc ataccgcccc cttgttgtga gttcatgaac
    34021 ctgacagttc aagtataagt cagcttgtga ttattttttg ttcgctcatg taatttgcac
    34081 cgtctgatgt acgtcctttt tcagcgtgaa ctattcacag ggtgtatgct tacaggcaag
    34141 caaagggaga cagactggcc tatggatctc aatacactca tctcacaata tggttatgcc
    34201 gcgctggtga tcggtagcct ggcggaaggt gaaaccgtga ctttgctggg aggcgttgcg
    34261 gcacatcagg ggctattaaa gttcccgctg gtggtacttt ctgtggcgct tggcggcatg
    34321 attggcgacc aggtgcttta tctgtgcggg cggcgctttg gcggcaagct attacgccgt
    34381 ttctcgaaac atcaggataa aattgagcgg gcgcagaaac ttatccaacg ccatccgtat
    34441 ctgtttgtca ttggtacgcg ttttatgtat ggctttaggg tgattggccc gacgctgatt
    34501 ggtgccagcc agctgccgcc gaaaatcttt ctgccgctga atattctcgg cgcatttgcc
    34561 tgggcgttga tttttaccac tatcggttat gctggtggtc aggtgattgc gccgtggttg
    34621 cacaatctcg accagcattt gaagcactgg gtctggttga ttctggccgt ggttctggtg
    34681 gtaggcgtgc gctggtggct gaaacgacgc gggaagaaaa agccggataa tcaggcgtaa
    34741 agcagttgcc acagataagc attcacgcca catccggcac cggatgttcc atgcctgatg
    34801 cgacgctggc gtgtcttatc tggcctaacc cattgaaata accacgcacc tcactgcgct
    34861 attctgggtt gaactgcgga ttcgccaaca taaagccgcc atccactatc aacgactgcc
    34921 cggtggtgta atttgcgccc tccgaacaaa gccataccac caggctggca atctcatgcg
    34981 ttgtgccaaa acgtcgcaag ggaatcgaag gctccgcgtc gggcctcaca tcgctatcat
    35041 ccatgccgtt cattggcgtg gcgatcgccc caggtgcgac tgcgttcacc aaaattttat
    35101 gcctgaccaa ctccagcgcc attgctttgg ttaacccgcc gagcgcatgt ttagctgctg
    35161 tgtaagcgct ggcatccggc agcggcgtat gttcatgcac cgacgtaatg ttgatgatgc
    35221 gaccgccctg cccttgtttc accatctgac gagccgcaat ttgcgagcat aagaatgcac
    35281 cgtcgacatc aacggtaaaa atcttgcgcc actcatcaaa agccatatca agaaacggcg
    35341 ctttggtcat tgcacccgca ttattcacca gcacatcgat gcgccccagt cgttgaatga
    35401 gtttctccag cgcctgcgca ccttctggca gtttaccgag atccagctgc acgatctccg
    35461 cacgtgcgcc gtggctaacc acctcacgcg cagtatcttt tgccccttct tcatcagagt
    35521 gccaggtaat gccaatatca aacccctgct gcgccagtaa taacgcgcac tctttgccga
    35581 tccccgaatc ggaggcggta ataatcgcaa cctgtgccat cgagttctcc acttaacgct
    35641 gaataaacgt taagtataga aggcgcatat catcagcgtt tgtaaccccc gcccaacgca
    35701 ccagtgagtt gaatggaggc atccagccac tgcccttgca ataacaggcc attggcccgc
    35761 tcgcgaagcg ccgggattct ggcttcgctg acgcgggaac cggcaatgat gcccgcgtta
    35821 aagcgcgcct gtgcaagacc tactacacgc aaggcatcgc gctcaatttg cgcctgatgc
    35881 tggtttttct ccgccagtgt ctgaacctga ctggctgccc gcgccacgtc attcaccgcg
    35941 tcaaccaccg ctttgttgta gctggcgata gacaagttgc tttcggcttt tgcgatatcg
    36001 aggttggcgt taagacgacc actatcgaaa atgggtagcg tcaggcctgc cgtaacgccc
    36061 atttgctgcg cggaatgacg gaacagatcg cttaagtgca acgcatcctg ttgcaggaag
    36121 gccatcaggt tgatgtcagg ataaaatgcc gcttttgccg catcaatagt gcttaacgat
    36181 gactcaacgt accagtgcgc cgcctgcaaa tctgcccgcc gggccagtaa ggagtacccc
    36241 agttcatcag gaagctggct tgccactttc ggcaacgcga ccggattaag cttcaatgcc
    36301 tttgtctggt gatttgtaag tgcacttaac cgtgcctcaa taattttcat tttccccgcg
    36361 acatcgttga gctgctgccg ggttttgctg gcattaatat cggtttccac accttcaact
    36421 gaagaagtaa tcccgttctg atatagctgg cgatcggtgg cgataatggt gttctgctct
    36481 ttttcgattt gctgcaagac cgtgtttaac gccgcctggg tttgccactc ccagtacagg
    36541 cgggctacgc tgccagccag caattggcgg gtttgctcgc gttccgccgc tcgtgcttta
    36601 accgtaccca ggcgagcagt tacctccgcc cgattctttc cccagatatc gagatgccag
    36661 cccgccgtta agccaaaagt accgttggtg taccacggac cggtcgtacc tgcggccgga
    36721 tcgttcagag caaacggccc cattaagcct tctgccgaca ttttttgccg ctccatatcc
    36781 gcagaaaagt cgagctgcgg accatcctga gtagcaattg ccttcgcctg ggcttcagcc
    36841 agctgaatgc gctgttcagc cacctgcata accggtgcgt tctgtagtgc gttattaatt
    36901 aatgaggtga gttgattatc gtgatactcc agccaccatt ggctgtctgg ccaaccattt
    36961 ttcagcgccg tgggtaatgc ggtgtcaact tgcacagcgg gcgtgtgctg gcttaacgcc
    37021 tggcgggttt catgcatagg cgcacacccg gccagcatca gtaacagcgg aaaacaggcg
    37081 atggctggat aaaaggaatc acgattcatg ggggaataat caggtaagaa aaggtgcgcg
    37141 gagattaccg tgtgttgcga tatatttttt agtttcgcgt ggcaatacat cagtggcaat
    37201 aaaacgacat atccagaaaa atatacacca agtgaatgat atcttccgat ttatcttaat
    37261 cgtttatgga tagcggcaaa gggcttcgtt ttttcctata cttattcagc actcacaaat
    37321 aaaggaacgc caatgaaaat tatactctgg gctgtattga ttattttcct gattgggcta
    37381 ctggtggtga ctggcgtatt taagatgata ttttaaaatt aattaatgtc atcagatccg
    37441 aaaataacga gaatatttca gtctctcatc ctgttgcgct cctgtcatgt gcattgcttc
    37501 atataatcac tggcgca
//
LOCUS       DAZGQL010000046        37244 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.046, whole genome shotgun sequence.
ACCESSION   DAZGQL010000046 DAZGQL010000000
VERSION     DAZGQL010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37244
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.046"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..385
                     /locus_tag="ACHKIR_002548"
     CDS             <1..385
                     /locus_tag="ACHKIR_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFO9990272.1"
                     /translation="LLTAEKARGMKGDEINAWRNAMEAANYMFEHNGRKWDYGKSTQT
                     RLEPSVAAAKAGKLPEAFFWTDAENNDVEVTAEELIALSEAAEQAMFTKGMEIHVRQR
                     TMKKELEKLTSADEILAYRVGWAQE"
     gene            complement(544..873)
                     /locus_tag="ACHKIR_002549"
     CDS             complement(544..873)
                     /locus_tag="ACHKIR_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFO9990273.1"
                     /translation="MNNPNPCMTCGACCAFFRVSFYWAEADDAGGKVPVSLTEQISPF
                     HRCMRGTSQKNPRCVALAGTPGKNAYCSVYKNRPSTCREFAMSGENGIANEACNRARA
                     KYGLPEI"
     gene            complement(1013..1100)
                     /locus_tag="ACHKIR_002550"
     tRNA            complement(1013..1100)
                     /locus_tag="ACHKIR_002550"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1064..1066),aa:Ser,seq:tga)
     gene            1527..2645
                     /gene="hyaA"
                     /locus_tag="ACHKIR_002551"
     CDS             1527..2645
                     /gene="hyaA"
                     /locus_tag="ACHKIR_002551"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HFO9990274.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     KMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            2642..4435
                     /gene="hyaB"
                     /locus_tag="ACHKIR_002552"
     CDS             2642..4435
                     /gene="hyaB"
                     /locus_tag="ACHKIR_002552"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HFO9990275.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLSDDGSELISVQVR"
     gene            4454..5161
                     /gene="hyaC"
                     /locus_tag="ACHKIR_002553"
     CDS             4454..5161
                     /gene="hyaC"
                     /locus_tag="ACHKIR_002553"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HFO9990276.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMVFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDVHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            5158..5745
                     /gene="hyaD"
                     /locus_tag="ACHKIR_002554"
     CDS             5158..5745
                     /gene="hyaD"
                     /locus_tag="ACHKIR_002554"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HFO9990277.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQSALAQLAAWGIVP
                     QPAKESRCLNYDCLSMENYEGVRLRQYRMRLEEQG"
     gene            5742..6140
                     /gene="hyaE"
                     /locus_tag="ACHKIR_002555"
     CDS             5742..6140
                     /gene="hyaE"
                     /locus_tag="ACHKIR_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HFO9990278.1"
                     /translation="MSNDTPFNALWQRMLARGWTPVSECRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLREFPDYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGS
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            6137..6994
                     /gene="hyaF"
                     /locus_tag="ACHKIR_002556"
     CDS             6137..6994
                     /gene="hyaF"
                     /locus_tag="ACHKIR_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HFO9990279.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQQQISERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRRRRGDKLLEDKLEAGCAPLALWQAATQNVLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEGAPT"
     gene            7128..8672
                     /gene="appC"
                     /locus_tag="ACHKIR_002557"
     CDS             7128..8672
                     /gene="appC"
                     /locus_tag="ACHKIR_002557"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HFO9990280.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            8684..9820
                     /gene="appB"
                     /locus_tag="ACHKIR_002558"
     CDS             8684..9820
                     /gene="appB"
                     /locus_tag="ACHKIR_002558"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFO9990281.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            9833..9925
                     /gene="cbdX"
                     /locus_tag="ACHKIR_002559"
     CDS             9833..9925
                     /gene="cbdX"
                     /locus_tag="ACHKIR_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HFO9990282.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            10005..11303
                     /gene="appA"
                     /locus_tag="ACHKIR_002560"
     CDS             10005..11303
                     /gene="appA"
                     /locus_tag="ACHKIR_002560"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HFO9990283.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQCLVADGLLAKKGCPQP
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFSQSNLCLNREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(11423..13603)
                     /gene="etk"
                     /locus_tag="ACHKIR_002561"
     CDS             complement(11423..13603)
                     /gene="etk"
                     /locus_tag="ACHKIR_002561"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HFO9990284.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIVSVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDMVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEGFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(13623..14069)
                     /gene="etp"
                     /locus_tag="ACHKIR_002562"
                     /gene_synonym="yccY"
     CDS             complement(13623..14069)
                     /gene="etp"
                     /locus_tag="ACHKIR_002562"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HFO9990285.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            complement(14057..15196)
                     /gene="gfcE"
                     /locus_tag="ACHKIR_002563"
     CDS             complement(14057..15196)
                     /gene="gfcE"
                     /locus_tag="ACHKIR_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HFO9990286.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(15242..17338)
                     /gene="gfcD"
                     /locus_tag="ACHKIR_002564"
     CDS             complement(15242..17338)
                     /gene="gfcD"
                     /locus_tag="ACHKIR_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFO9990287.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFDAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEPLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            complement(17338..18084)
                     /gene="gfcC"
                     /locus_tag="ACHKIR_002565"
     CDS             complement(17338..18084)
                     /gene="gfcC"
                     /locus_tag="ACHKIR_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFO9990288.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPVEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVVATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWLAGRSVTDYLQDHTRLAGADKNNVMVITPEGEAVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(18081..18725)
                     /gene="gfcB"
                     /locus_tag="ACHKIR_002566"
     CDS             complement(18081..18725)
                     /gene="gfcB"
                     /locus_tag="ACHKIR_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HFO9990289.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGRDV
                     LCNVWQEEVTSTLPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(18832..19137)
                     /locus_tag="ACHKIR_002567"
     CDS             complement(18832..19137)
                     /locus_tag="ACHKIR_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9990290.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEAANGTEATTGTTGTTTTTTG
                     ATTTAATTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            complement(19578..19790)
                     /gene="cspH"
                     /locus_tag="ACHKIR_002568"
     CDS             complement(19578..19790)
                     /gene="cspH"
                     /locus_tag="ACHKIR_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HFO9990291.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            20076..20288
                     /gene="cspG"
                     /locus_tag="ACHKIR_002569"
     CDS             20076..20288
                     /gene="cspG"
                     /locus_tag="ACHKIR_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HFO9990292.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            20299..20487
                     /gene="ymcF"
                     /locus_tag="ACHKIR_002570"
     CDS             20299..20487
                     /gene="ymcF"
                     /locus_tag="ACHKIR_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFO9990293.1"
                     /translation="MTPHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            20462..20692
                     /gene="ymcE"
                     /locus_tag="ACHKIR_002571"
     CDS             20462..20692
                     /gene="ymcE"
                     /locus_tag="ACHKIR_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HFO9990294.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLSRPEEVNSEY"
     gene            20682..20855
                     /gene="gnsA"
                     /locus_tag="ACHKIR_002572"
     CDS             20682..20855
                     /gene="gnsA"
                     /locus_tag="ACHKIR_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HFO9990295.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(20903..21976)
                     /gene="yccM"
                     /locus_tag="ACHKIR_002573"
     CDS             complement(20903..21976)
                     /gene="yccM"
                     /locus_tag="ACHKIR_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFO9990296.1"
                     /translation="MTEKRRTRWQRRPGTTGGKLPWNDWRNATTWRKATQLLLLAMNI
                     YIAITFWYWVRYYETAGSTTFVTRPGGIEGWLPIAGLMNLKYSLATGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQFVLPRWLDTPLRGVKYL
                     LLSFFLYIALLMPAQAIHYFMLSPYSVVMDVKMLNFFRHMGTATLISMIVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKNCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLILGIMFAVIGYAMYAG
                     VWQSPVPEELYRRLIPQAPMIGH"
     gene            complement(22048..24792)
                     /gene="torS"
                     /locus_tag="ACHKIR_002574"
     CDS             complement(22048..24792)
                     /gene="torS"
                     /locus_tag="ACHKIR_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HFO9990297.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     DFDTTAIEQQEQEISRSLRQQGELVGQRLQLRQQQQQLSQQIVAAADEIARLAQGQAN
                     NAATSAGATQAGIYDLIEQHQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRTQVATTLTTVSQYSDLLALY
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHLEKASARGQYSLL
                     LLGIVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     RLMDAFRSSVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKANQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDVPTALMGDPQRIRQVI
                     TNLLSNALRFTDEGHIILRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLLLRIATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNSSGAQVVAVGNAAQALETLQNSEPFAVALVDFDLPDID
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQVNNDQPLDVSQLNEDAQLMGTEKIHEWLVLFTQHALPLLDDIDIARANQDSEKIK
                     RAAHQLKSSCSSLGMRIASQLCAQLEQQPLSAPLPHEEITRSVAALEAWLHKKDLNAI
                     "
     gene            24875..25903
                     /gene="torT"
                     /locus_tag="ACHKIR_002575"
     CDS             24875..25903
                     /gene="torT"
                     /locus_tag="ACHKIR_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HFO9990298.1"
                     /translation="MRVLLLLLLSLFMLSAFSADNLLRWHDAQHYTVQASTPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNAIDAPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGNPLNVLLMPGPDNAGGSKEMAEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQEQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(25876..26568)
                     /gene="torR"
                     /locus_tag="ACHKIR_002576"
     CDS             complement(25876..26568)
                     /gene="torR"
                     /locus_tag="ACHKIR_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HFO9990299.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            26698..27870
                     /gene="torC"
                     /locus_tag="ACHKIR_002577"
     CDS             26698..27870
                     /gene="torC"
                     /locus_tag="ACHKIR_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HFO9990300.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHAKQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEISHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            27870..30416
                     /gene="torA"
                     /locus_tag="ACHKIR_002578"
     CDS             27870..30416
                     /gene="torA"
                     /locus_tag="ACHKIR_002578"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0050626 - trimethylamine-N-oxide reductase
                     (cytochrome c) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HFO9990301.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRRATAAQAAT
                     EAVISKEGILTGSHWGAIRATVKEGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYEQLKAKVAAGEIEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAHTLYSENLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGINAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIP
                     IARFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVI
                     AIDNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDI
                     FRELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFD
                     HPQMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGG
                     PGSQKYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFINPQDASARGIRNGDV
                     VRVFNARGQVLAGAVVSDRYAPGVARIHEGAWYDPDKGGEPGALCKYGNPNVLTIDIG
                     TSQLAQATSAHTTLVEIEKYNGTVEQVTAFNGPVEMVAQCEYVPASQVKS"
     gene            30413..31012
                     /gene="torD"
                     /locus_tag="ACHKIR_002579"
     CDS             30413..31012
                     /gene="torD"
                     /locus_tag="ACHKIR_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HFO9990302.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLTAAVNELENRIATQTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPADHLAIYLELLSHLHFSLGEGTVPARRIDNLRQKTLTA
                     LRQWLPEFAARCHQYDSFGFYAALSQLLLVLVECDHQNR"
     gene            complement(31267..31572)
                     /gene="cbpM"
                     /locus_tag="ACHKIR_002580"
     CDS             complement(31267..31572)
                     /gene="cbpM"
                     /locus_tag="ACHKIR_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HFO9990303.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(31572..32492)
                     /gene="cbpA"
                     /locus_tag="ACHKIR_002581"
     CDS             complement(31572..32492)
                     /gene="cbpA"
                     /locus_tag="ACHKIR_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HFO9990304.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPATRGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGTKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            32645..32746
                     /locus_tag="ACHKIR_002582"
                     /pseudo
     CDS             32645..32746
                     /locus_tag="ACHKIR_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415521.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            33027..34268
                     /gene="agp"
                     /locus_tag="ACHKIR_002583"
     CDS             33027..34268
                     /gene="agp"
                     /locus_tag="ACHKIR_002583"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HFO9990305.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYKLLEQIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDRVLNEAVK"
     gene            complement(34306..34533)
                     /gene="yccJ"
                     /locus_tag="ACHKIR_002584"
     CDS             complement(34306..34533)
                     /gene="yccJ"
                     /locus_tag="ACHKIR_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HFO9990306.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(34554..35132)
                     /gene="wrbA"
                     /locus_tag="ACHKIR_002585"
     CDS             complement(34554..35132)
                     /gene="wrbA"
                     /locus_tag="ACHKIR_002585"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFO9990307.1"
                     /translation="MINHILRHVETMARAVAEGASKVDGAEVVVKRVPETMPPQLFEK
                     AGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGALYGKLA
                     SVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPYGATTIA
                     GGDGSRQPSQEELSIARYQGEYVAGLAVKLNS"
     gene            complement(35129..36439)
                     /locus_tag="ACHKIR_002586"
     CDS             complement(35129..36439)
                     /locus_tag="ACHKIR_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO9990308.1"
                     /translation="MANSAYPAGVENHGGKLRITFKYRGKRVRENLRVPDTPKNRKIA
                     GELRASVCFAIRTGTFDYAERFPDSPNLKLFGLVKKDITVGELAQKWLTLKAMEISSN
                     ALNRYQSVMKNMLPRLGPGRLASSITKEDLLFIRKDLLTGEKGSRKTSTSQKGRTVPT
                     VNYYMTTTAGMFSFAAENGYLEKNPFNSITPLRKSKPVPDPLTRDEFSRLIDACHHQQ
                     TKNLWTVAVFTGMRHGEIAALAWEDIDLKAGTITVRRNFTKIGDFTLPKTDAGTNRVI
                     HLLAPAIEALKNQAMLTRLGRQHQITVKLREYGRTILHECTFVFCPQIVRKNHKAGIN
                     YAVSSIGATWDSAIKRAGIRSRKAYQSRHTYACWALSAGANPTFIASQMGHSSASMVY
                     NVYGAWMPECSVTQVAMLNNILNSRAPDVPQSDQEDEIKLYFSK"
     gene            complement(36492..36776)
                     /locus_tag="ACHKIR_002587"
     CDS             complement(36492..36776)
                     /locus_tag="ACHKIR_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFO9990309.1"
                     /translation="MRELVNQHNHGIQPVITPVVQINANEWVTLELLMAVTGLRKGTI
                     LRARDSAWMNGREYKQIAPDGTPKKNSECLYHLPTINTWIKNQPLPSQDV"
     gene            complement(36822..37073)
                     /locus_tag="ACHKIR_002588"
     CDS             complement(36822..37073)
                     /locus_tag="ACHKIR_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO9990310.1"
                     /translation="MFVLIQRGQSFVDANNYPVEICKVTLTQVIYRRLDGRTRATSIS
                     AFNEEFERIEHNELHMIKAEIEKEKHIASLRKMRRTSIN"
     gene            complement(37103..>37244)
                     /locus_tag="ACHKIR_002589"
     CDS             complement(37103..>37244)
                     /locus_tag="ACHKIR_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000994786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFO9990311.1"
                     /translation="NIVGDLLRKSQKEARRAGRKQKRWEEACKALRELNKYRDLINEL
                     AE"
BASE COUNT         8824 a         9447 c         9771 g         9202 t
ORIGIN      
        1 cctgctgacc gcggagaaag cgcgagggat gaagggcgat gaaattaacg cctggcgtaa
       61 tgcgatggaa gcggcgaact acatgtttga gcacaatggg cgtaaatggg actacgggaa
      121 gtcaacgcag acgcgtcttg agccgtcggt ggcggcagcg aaagcgggga aactgccgga
      181 ggcgtttttc tggacggatg cggaaaacaa tgatgtggag gtgacagcag aagagcttat
      241 agcactgagt gaagcggcag agcaggcgat gttcaccaaa gggatggaaa tccacgtccg
      301 gcagcgcacc atgaagaaag agctggaaaa gctgaccagt gcggatgaga tactggcata
      361 cagggttggc tgggcacagg aataacagac aaaaagatgg gggaccatca cctcccccat
      421 gaactgagtc aattatacac ttgtgaagta tttactatgc gaataaatac tgtcacggaa
      481 agtgcaggcc tggatgtgat ggaaatcatg tttccggcgc atcctgttga tatggataat
      541 ttttcagatt tccggaagtc cgtattttgc ccgtgcgcgg ttacatgctt cattcgcgat
      601 gccattttcg ccggacattg cgaattcccg gcatgtggac ggacggttct tgtaaacaga
      661 gcaatatgcg tttttacccg gagttcccgc cagagcgaca catcggggat ttttctgact
      721 ggtgccgcgc atacagcgat gaaaggggga tatttgctca gtgagactga ccggaacctt
      781 tccgccagca tcgtcagctt ctgcccagta aaaagagacg cggaaaaatg cacaacaggc
      841 accacacgtc atgcatggat tggggttatt cataattcat cggtatagcg ataaatcgta
      901 aagagaacaa tgtacctagt gtgtattgag ctaaatatac caattagtat accaagtgta
      961 tactaagagc aaaaaaagaa aggggctacc atttggtaac cccttgattg tttggcggaa
     1021 gcgcagagat tcgaactctg gaaccctttc gggtcgccgg ttttcaagac cggtgccttc
     1081 aaccgctcgg ccacacttcc ggaatgacgc gcactataaa catcccgatg cggcgtgtaa
     1141 acccctaatt tgtttgtttg cctgaaaaac agccaaaagt gcattgatag catgaaataa
     1201 cagcagattg atcatttcat caccatgaat tccttctctt ttactcgttt agcaaccggc
     1261 taaacatccc cactgcccgg caaaaagaaa aataggtcca tttttatcgc taaaagataa
     1321 atccacacag tttgtattgt tttgtgcaaa agtttcacta cgctttatta acattacttt
     1381 ctggcgacgt gcgccagtgc agaaggatga gctttcgttt tcagcatctc acgtgaagcg
     1441 atggtttgcc ttgctacagg gacgtcgctt gccgaccata agcgcccggt gtcctgccgg
     1501 tgtcgcaagg agaagagacg tgcgatatga ataacgagga aacattttac caggccatgc
     1561 ggcgtcaggg cgttacccgg cgcagctttc tcaaatattg tagtctggct gccacgtcgc
     1621 tgggattagg cgcgggaatg gcaccaaaga ttgcctgggc gctggagaac aaaccgcgca
     1681 ttccggtggt atggatccac ggtctggaat gcacctgctg taccgaatct tttatccgct
     1741 ccgctcaccc actggcgaag gacgtcatcc tttccctgat ttccctcgat tatgacgata
     1801 ctttgatggc tgccgccgga acccaggcgg aagaagtctt tgaagacatc atcacgcaat
     1861 acaatggcaa atatatcctc gcagtagaag gtaatccgcc gctgggcgag caggggatgt
     1921 tctgtatcag cagcggacga ccgtttattg agaaactcaa acgtgccgct gccggagcca
     1981 gtgcgattat cgcctgggga acctgcgcgt cctggggctg cgtgcaggcc gcgcgaccca
     2041 atccgacgca ggcaacgcct atcgacaaag tcatcaccga caaacccatt atcaaagtac
     2101 ctggctgccc gccgatcccg gatgtgatga gcgccatcat tacttacatg gtgacctttg
     2161 atcgcttgcc agatgtcgac aaaatgggcc gtccactgat gttttatggt cagcgtatcc
     2221 acgataaatg ttatcgccgc gctcacttcg acgccggaga gttcgtccag agttgggatg
     2281 atgacgctgc ccgcaaaggt tactgcctgt acaaaatggg ctgcaaaggg cctaccacct
     2341 ataacgcctg ttcctccaca cgctggaacg atggcgtttc cttcccgatc cagtcaggtc
     2401 acggctgcct gggttgtgcg gaaaatggtt tctgggatcg cggttcgttc tacagccgcg
     2461 tggtcgatat tccgcaaatg ggtacccatt ccaccgccga taccgtcggt ttaaccgcac
     2521 ttggcgtggt ggcagcggct gttggtgtgc acgcagtcgc cagcgccgtt gaccagcgca
     2581 gacgccataa ccagcaacct acagaaaccg aacatcagcc aggcaatgag gataaacagg
     2641 catgagcact cagtacgaaa ctcagggata caccatcaat aatgccggac gccgcctggt
     2701 ggtcgacccg attacgcgca ttgaaggcca catgcgctgc gaagtgaata ttaacgatca
     2761 gaatgtgatc accaatgccg tctcctgcgg caccatgttt cgtgggctgg agatcatcct
     2821 gcaagggcgc gacccgcgcg atgcgtgggc gttcgttgaa cgtatctgcg gcgtctgtac
     2881 tggcgtacac gctctggctt cggtttacgc catcgaagat gccatcggta ttaaagtgcc
     2941 ggacaacgcc aatatcatcc gcaacattat gctggcaacg ctctggtgcc acgatcatct
     3001 ggtgcacttc tatcagcttg ccgggatgga ctggatcgat gtgttagatg cgctgaaagc
     3061 cgacccgcgg aaaacctccg aactggcgca aagcctctcc tcatggccga aatcatcccc
     3121 tggctatttc ttcgacgtac aaaaccgcct gaagaaattc gttgaaggcg ggcagttggg
     3181 gatcttccgc aatggctact gggggcaccc gcagtacaaa ctgccgccag aagccaacct
     3241 gatgggcttt gcccactatc ttgaagctct cgatttccaa cgtgaaattg tcaaaatcca
     3301 cgcggtcttt ggcggtaaaa acccgcatcc aaactggatt gtcggcggga tgccttgtgc
     3361 cattaacatt gacgaaagcg gcgcggtcgg ggcggtcaat atggaacgcc tgaacctggt
     3421 gcagtcaatc attacccgta cggcggattt cattaacaac gtgatgatcc ccgacgcctt
     3481 agccatcggt cagttcaaca agccgtggag cgaaatcggc actggccttt cggataaatg
     3541 cgttctcagt tacggcgcgt tcccggacat cgccaacgac tttggcgaga aaagtctact
     3601 gatgcctggc ggcgcggtga ttaacggcga cttcaacaat gtgctgccag tggatttggt
     3661 tgatccgcag caggtgcagg agtttgtcga tcacgcctgg tatcgttatc ccaacgatca
     3721 ggtcgggcgt catccgttcg atggcattac cgacccgtgg tacaaccccg gcgatgtcaa
     3781 aggcagcgat accaacattc agcagctgaa tgaacaggaa cgctactcgt ggatcaaagc
     3841 gccacgctgg cgcggtaatg cgatggaagt ggggccgctg gcacgcacgt taatcgctta
     3901 tcacaaaggc gatgcggcga ccgttgagtc ggtcgatcgc atgatgtcgg cgttgaacct
     3961 gccgctttcc ggcatccagt caacattagg ccgcattttg tgtcgcgcgc acgaagcgca
     4021 gtgggccgca ggcaagttgc aatatttctt cgacaagctg atgaccaatc tcaaaaacgg
     4081 caatctcgcc accgcctcca ctgagaagtg ggaaccagca acctggccga cagagtgccg
     4141 tggtgtcggc tttaccgaag cgccgcgcgg agcgttaggc cactgggccg ccattcgcga
     4201 tggcaagatt gatctctacc agtgcgtggt gccgaccacc tggaacgcca gcccgcgcga
     4261 tcctaaaggg caaattggcg cttatgaagc ggcgctgatg aacaccaaaa tggcgatccc
     4321 cgagcaaccg ctggagatcc tgcgtactct gcacagcttt gatccgtgcc tcgcctgttc
     4381 aacacacgtg ctgagcgacg acggtagcga gctgatctcc gtgcaggtgc gttaacagcg
     4441 aaggagaatc atcatgcaac agaaaagcga caacgttgtc agccactatg tctttgaagc
     4501 gccagtgcgt atctggcact ggctgacggt gttatgcatg gcggtgttga tggtcaccgg
     4561 ttactttatc ggcaagccgc taccttccgt cagcggcgag gcgacgtatc tgttctatat
     4621 gggctacatc aggttaattc acttcagcgc cgggatggtt tttaccgtgg tcttgctgat
     4681 gcggatctac tgggcttttg tcggcaatcg ctactcacgc gagctgttta tcgtgccagt
     4741 atggcgtaaa agctggtggc agggcgtgtg gtatgaaatc cgctggtatc tgtttctggc
     4801 aaaacgtccg agtgccgata taggtcataa ccccatcgcc caggcggcga tgttcggcta
     4861 tttcctgatg tcagttttta tgatcatcac cggctttgcg ctgtacagcg aacacagcca
     4921 gtacgctatt tttgcgccgt tccgttatgt ggtggaattt ttctactgga cgggcggcaa
     4981 ctcaatggac gttcacagct ggcatcggct ggggatgtgg ctgattggcg cgtttgtgat
     5041 cggtcatgtc tacatggcgc tgcgtgaaga tatcatgtcc gacgacacgg tgatctccac
     5101 catggtcaac ggctaccgta gccacaaatt tggcaaaata agtaacaagg agcgttcatg
     5161 agcgagcaac gcgtggtggt aatggggctg ggcaacctgc tgtgggccga tgaaggcttc
     5221 ggcgtgcggg tggcggaacg gctgtatgcc cattaccact ggcccgagta tgtggagatt
     5281 gtcgatggcg gtactcaggg actgaacttg ctggggtatg tcgaaagcgc cagtcacttg
     5341 ttgattctcg atgccattga ctacgggctg gaaccaggaa cgctgcgaac ctatgccgga
     5401 gaacgcattc cggcttatct cagcgcgaag aaaatgagcc tgcaccagaa cagcttctcc
     5461 gaagtgctgg cgctggcgga tatacgtggt catttgccag cacatattgc cctcgtcggt
     5521 ctgcaacccg caatgctcga cgactacggc ggtagcctga gcgaactggc gcgggagcaa
     5581 ctgcccgctg cggaacaatc ggcgctggcg cagctggctg cgtggggaat tgtgccgcaa
     5641 ccggctaagg aatcgcgctg tctcaattat gactgtctgt cgatggaaaa ttacgaaggc
     5701 gttcgcttgc gtcagtacag gatgaggctg gaggaacagg gatgagcaac gacacaccat
     5761 ttaatgcgtt gtggcaacga atgctggcgc gcggctggac gccggtcagt gaatgccgtc
     5821 ttgacgactg gcttacgcaa gcgccagacg gcgtggtgtt attaagtagt gacccgaaac
     5881 gcacgccaga agtcagcgat aacccggtaa tgattggcga attactgcgc gagtttcccg
     5941 actatacatg gcaggtagcg attgctgatc ttgagcagag cgaagccatc ggtgatcgtt
     6001 ttggcgtatt tcgttttccc gccacactgg tgtttaccgg tggtagctat cgcggcgtgc
     6061 tgaatggtat tcacccgtgg gcggaactga taaacctgat gcgtgggctt gtcgaaccac
     6121 aacaggagcg tgcctcatga gcgaaacttt tttccatctg ttggggccag gaacgcaacc
     6181 gaacgatgac agtttcagca tgaatccact gccgatcacc tgtcaggtga atgatgaacc
     6241 gagtatggcg gccctggagc aatgtgctca cagcccgcag gtgattgcgc tgttaaacga
     6301 gttacaacaa caaataagcg aacgccagcc accgttgggc gaggtgctgg cagtcgatct
     6361 gttaaatctc aacgccgacg atcgtcactt tatcaatacg cttctcgggg aaggggaagt
     6421 gtcagtgcgc atacagcagg ctgacgacag tgaaagtgaa atacaggagg cgatcttctg
     6481 cggattatgg cgggtgcgca gacgtcgcgg cgacaagttg ctggaggaca aactggaggc
     6541 tggctgcgcg ccgctggcat tgtggcaggc ggcaacgcaa aacgtcttgc cgacagattc
     6601 gctgttaccg ccgcccattg atggcctgat gaatggccta ccgttggcgc atgagctact
     6661 ggcgcatgta cgtaaccccg acgcgcagcc gcacagcatt aatctgacgc aattacccat
     6721 cagcgaggct gatcggcttt ttctctcacg tctctgtggg ccgggaaata ttcagattcg
     6781 caccattggc tatggcgaga gctatatcaa cgccacgggg ttacgccatg tctggcattt
     6841 acgctgcacg gacaccttaa aaggcccgtt actggaaagt tatgaaatct gcccaatacc
     6901 ggaagtggtg ctggcagcgc cagaagattt ggtcgactct gcgcagcggc ttagcgaggt
     6961 atgccagtgg ctggcggaag gtgcaccgac gtaaaaagac ggtaagtatc gctttcaggc
     7021 ttatgaatat cgcaatcggc gaatacctct ggtcgtagag tttcaggata aagagggaga
     7081 tctaccatta acgggttatt tttctctctt cgcctacagg agtgcgcatg tgggatgtca
     7141 ttgatttatc gcgctggcag tttgctctga ccgcgctgta tcacttttta tttgtacccc
     7201 ttaccctggg gctgattttt ttgctggcta tcatggaaac catttacgtg gtcaccggca
     7261 aaacaatcta ccgcgatatg acgcgcttct ggggtaagct cttcggtatc aactttgctc
     7321 ttggcgtggc taccggcctg acgatggagt ttcagtttgg tactaactgg tcattctatt
     7381 ccaactatgt gggcgatatt tttggcgcac cgctggcgat ggaagcatta atggccttct
     7441 tcctcgaatc cacctttgtc gggctgttct tcttcggctg gcagcggctg aataaatacc
     7501 agcacctgct ggtgacgtgg ctggtggcgt tcggttcaaa tctctctgca ttgtggatat
     7561 tgaatgccaa cggttggatg caatacccga ccggtgcgca ttttgatatc gacaccctgc
     7621 gtatggagat gaccagtttc agcgagttgg tctttaaccc ggtcagccag gtgaaatttg
     7681 tgcacactgt aatggcgggc tacgtgaccg gggccatgtt tattatggcg atcagcgcct
     7741 ggtatttact gcgcggacgg gagcgcgatg ttgcattacg ctcgtttgcc atcggttccg
     7801 tcttcggtac tctggcgatt atcggcacgc tgcaactcgg agacagttct gcgtatgaag
     7861 tcgcgcaagt acaaccggta aaactggcgg cgatggaagg ggagtggcaa acggaacctg
     7921 cacctgcacc gttccatgtg gttgcctggc cggaacagga tcaagagcgt aacgcctttg
     7981 ctatcaaaat tcccgcgctg ctagggatcc tcgccactca ctcattagat aaacccgtgc
     8041 cgggtctgaa gaatttgatg gctgaaacct acccacgctt gcaacgcgga cgtatggcct
     8101 ggctgttaat gcaggaaata tcgcaaggca atcgtgagcc gcatgtgttg caggcatttc
     8161 gggagctgga aggcgacctt ggttacggca tgttgctctc ccgctatgcg ccggatatga
     8221 accatgtcac agctgcacag taccaggcgg cgatgcgtgg cgcgatacct caggttgcgc
     8281 cggtattctg gagtttccgc atcatggtgg gctgtggttc tctgctgcta ctggtgatgc
     8341 tgattgcgct tgtccagacg ctgcgtggca aaattgacca gcatcgctgg gtgctgaaaa
     8401 tggcgctctg gagtttgccg ctgccgtgga ttgcgattga agccgggtgg tttatgaccg
     8461 agtttggtcg tcagccgtgg gcgatacagg acatcttacc gacatactcc gcgcactccg
     8521 ccttaaccac aggacaactg gctttctcac tgatcatgat cgtagggctt tacaccctgt
     8581 tcttaatcgc cgaagtctac ctgatgcaga aatatgcccg tctggggccg agcgcgatgc
     8641 agagtgaaca accgacgcag caacaggggt aaaggagaaa atcatgtttg attatgaaac
     8701 attgcgcttc atctggtggc tgctgattgg cgtgatcctg gtggtcttta tgatctccga
     8761 cggatttgac atggggatcg gctgtctgct gccgctggtg gcgcgtaatg atgatgaacg
     8821 tcggatagtg ataaacagcg ttggcgcgca ctgggaaggc aatcaggtct ggttgatcct
     8881 cgcaggtggg gcattatttg ccgcctggcc cagagtgtat gcagcggcgt tttccggctt
     8941 ttatgtggcg atgattctgg tgctgtgctc gctgttcttc cgcccgctgg cctttgatta
     9001 tcgcggaaaa atcgccgatg cacgctggcg taaaatgtgg gatgccggac tggtcatcgg
     9061 cagtctggtg ccgccggtag tcttcggtat tgcattcggc aacttgctgc tcggcgtgcc
     9121 gtttgctttc acaccgcaat tacgcgtgga atatctcggc agcttctggc aactgctgac
     9181 gccattccct ttattgtgtg gattgctcag tcttgggatg gtgattttgc aaggtggcgt
     9241 ctggttacaa ctgaaaactg ttggtgtgat tcatctgcgt tcacagctgg cgaccaaacg
     9301 cgccgcacta ctggtgatgc tgtgcttttt gctggcgggt tactggctgt gggttggtat
     9361 tgatggcttt gtactgctcg cccaggatgc taacggtcct tccaatccgt taatgaaact
     9421 ggtggcagtg ctacctggtg cctggatgaa taatttcgtc gaatcgcccg ttttgtggat
     9481 cttcccgctg ctggggttct tctgcccatt gctgacggtg atggcgattt atcgtggtcg
     9541 cccgggttgg ggatttttga tggcgtcatt gatgcaattt ggcgtgattt tcacggcagg
     9601 catcacgctg ttcccctttg tcatgccgtc aagcgtgagt ccgatctcca gcctgacgtt
     9661 gtgggacagc acttccagtc agctgacgct gagcattatg ttggtaatcg tgctgatatt
     9721 tttgcctatt gtgttgctct acactctctg gagctactac aaaatgtggg gacgcatgac
     9781 gacagaaact ctccgccgta acgaaaacga gctgtactaa ggagcaggaa caatgtggta
     9841 tttactttgg ttcgtcggca ttttgttgat gtgttcgctc tccacccttg tgttggtatg
     9901 gctggacccg cgtctgaaaa gttaacgaac gtaagcctga tgtggtgcat tagcatcgca
     9961 tcaggcaatc aataatgtca gatatgaaaa gcggaaacat atcgatgaaa gcgatcttaa
    10021 tcccattttt atctcttctg attccgttaa ccccgcaatc tgcattcgct cagagtgagc
    10081 cggagctgaa gctggaaagt gtggtgattg tcagtcgtca tggtgtgcgt gctccaacca
    10141 aggccacgca actgatgcag gatgtcaccc cagacgcatg gccaacctgg ccggtaaaac
    10201 tgggttggct gacaccgcgc ggtggtgagc taatcgctta tcttggacat taccaacgcc
    10261 agtgtctggt ggctgacgga ttgctggcga aaaagggctg cccgcagcct ggtcaggtcg
    10321 cgattattgc tgatgtcgac gagcgtaccc gtaaaacagg cgaagccttc gccgccgggc
    10381 tggcacctga ctgtgcaata accgtacata cccaggcaga tacgtccagt cccgatccgt
    10441 tatttaatcc cctaaaaact ggcgtttgcc aactggataa tgcgaacgtg actgacgcga
    10501 tcctcagcag ggcaggaggg tcaattgctg actttaccgg gcatcggcaa acggcgtttc
    10561 gcgaactgga acgggtgctt aatttttcgc aatcaaactt gtgccttaac cgtgagaaac
    10621 aggacgaaag ctgttcatta acgcaggcat taccatcgga actcaaggtg agcgccgaca
    10681 atgtctcatt aaccggtgcg gtaagcctcg catcaatgct gacggagata tttctcctgc
    10741 aacaggcaca aggaatgccg gagccggggt ggggaaggat cactgattca caccagtgga
    10801 acaccttgct aagtttgcat aacgcgcaat tttatttact acaacgcacg ccagaggttg
    10861 cccgcagtcg cgccaccccg ttattggatt tgatcatggc agcgttgacg ccccatccac
    10921 cgcaaaaaca ggcgtatggt gtgacattac ccacttcagt gctgtttatt gccggacacg
    10981 atactaatct ggcaaatctc ggcggcgcac tggagctcaa ctggacgctt ccaggtcagc
    11041 cggataacac accgccaggt ggtgaactgg tgtttgaacg ctggcgtcgg ctaagcgata
    11101 acagccagtg gattcaggtt tcgctggtct tccagacttt acagcagatg cgtgataaaa
    11161 cgccgctgtc attaaatacg ccgcccggag aggtgaaact gaccctggca ggatgtgaag
    11221 agcgaaatgc gcagggcatg tgttcgttgg caggttttac gcaaatcgtg aatgaagcac
    11281 gcataccggc gtgcagtttg taatgcataa aaaagagcat tcagttatct gaatgctctg
    11341 aggctgatga caaacgaaga actgtctaat gcgtagaccg gaaaaggcgt tcacgcccca
    11401 tccggccacc ttaccttcag ttttactctt tctcggagta actataaccg taatagttat
    11461 agccgtaact gtaagcggtg ctggcgcgtt taatcacacc attgaggatt gcgcctttaa
    11521 tattaacgcc agcctgttcc agacgctgca ttgacaagct cacctctttg gcggtgttca
    11581 agccaaaacg cgcaaccagc aggctggtgc cgacagagcg tcccacgacc gcagcatcgc
    11641 tcaccgccag catcggcggc gtatcgacaa tcaccagatc gtaatggtcg ttcgcccatt
    11701 ccagtaattg acgcatccga tcgcgcatca gcagttcaga cgggttaggt ggcacctgac
    11761 cgcgagtaat cacatcaaag cctcctttgc caaaatgctg gatcactttg ttgagctcat
    11821 ctttacctgc cagatattcc gacaagccat gttcattact cacggtaaac aggttatgcg
    11881 aataaccacg gcgtaagtcg gcatcaataa ataacacttt ttgatcggac tgggcgatca
    11941 ccgctgccag agttgaactg acgaacgttt taccactgtc tggcgtcgca ccggtgatca
    12001 tcagaatgtt attctctgtc tccatcatag cgaagtgcag actggttcgt agcgcacgta
    12061 cggcctccac agcagaatcc gccgggttat ccaccgccag gaaggggata tttttagtac
    12121 gatggcgctg ctgattagaa aataaatttt tcttacgcag acgggtacgt ttatccagcc
    12181 actcggacat tgggatagtg gcataaacgc tgatgccgtg ctcttccagt tgttccgggg
    12241 cttctacacc acgacgcaac atcgcacgcg ccagcacggc acccaccgaa ataaacaggc
    12301 caagaataaa accaagcacc acattcaacg cttttttcgg tttcactggc tgcggctgag
    12361 tgactgccgg gtcgataatc cgcacgttac caatcgcact ggatttcgaa atactcaact
    12421 cctgctggcg gttaagtaat tgcagatata ccgcacggcc cgcttctacg tcacgactta
    12481 aacgcaacac ttcctgttgg gtggaaggca ttgccgatac ccgcttattc aggcgtttgc
    12541 gttcttgctc cagtgtctgg cgtttttcca gcagcgcacg ataagttggg tgatctttct
    12601 tatacagctg ggagatctct gcctcgcgga aagtcagctc attgagttga ttatcaacgt
    12661 tcacaatctg ctcaagaacg gctttggctt ccaggttaag gtcaaccgaa tcgcgctgtt
    12721 ggcgataaac gttgagtttt tcttccgcct ggtccagctc gctgcgcact tctggcaact
    12781 ggcgctgtaa gaattcaagg ctttgtgaat cctgcgccgc ctggcgagcg atattctgtt
    12841 gcaaatagtt gttagcgatg ctgttcagaa tacgagtaat caactgggga tcatcaccgg
    12901 tcatggtaag ttccagcatc ccgctttctt tactgcgttc gctaacggta aaggtttcct
    12961 gcaacgcgtt aatcgcttcc agttcggtac gctggctcag gacaaactgc gttcctggtt
    13021 tggccttaat gtccgcgata gtcagcgcaa cgccgtcttt ttccagacgc tggccgacca
    13081 taccattgac ggtgaaccct tcaccttcca gcgtatagtg gccgttttcc ccaactgtga
    13141 gtgtcagttg ctgatcctga ccattcagtt gtgggatgtg catccagctg atcgccagct
    13201 cacctggttt ctctttggtt aatctcgccc agccgcgacc cacaatcgga aaatacttct
    13261 gctcaaccat gtcgcgcaga ttcagttcag caatggtttt accgagaatc atgcgcgatt
    13321 gcagcagttg gatctccggt gctgactcgg gcgatgagtt agggatcata tcgctcaggc
    13381 cgctgagaat ggcgttgccc tgtttctgct caacctggac cagagtatct gcctgataaa
    13441 ttggcgtgct taacagcgag taagcgacag cgatcagcgt gaacaacgcg gtcacgctga
    13501 caataaactt acggtgatcc cataactcgc cgaccagacg aagcagatcg atctcatttt
    13561 cctgagtgct gcctggtggc gtattcatat ttttagttgt catcgtattt tataaactca
    13621 aattaccggc tgaggcgctt cgcccattcc tgactggcgc gctccaacat accgtagaca
    13681 tgctcaaatg cgtcctgact tttacgatag ggatccggga tctctttctg ttccagccat
    13741 tgcccaaaca gcattgtctt cccgcggacc tcgggagcga ttgccgtaac ctgagcaata
    13801 tgttccgact ccattgccag aatcagatcg taatttcgtg ccatctcagc agtgagctta
    13861 cgtccggcat gtccttcaag agatacgcca tgattagcgg caacatctgc cgctgtcgca
    13921 tctgcagggt gctttaccag accatgaaca cctgccgatt tgacttttac gcctggcaga
    13981 cgtttacgca gcaagcgctc gccaatgggc gaacggcaaa tgttgccggt acaaaccacc
    14041 aggattgagt taaattttag ttgggccatg tttgaatcca ccgtgaagtc tctgtcaggt
    14101 catgcacccc ggaaatcgtc ggtaccagta gagaaatcac acggttccaa cgagccagcg
    14161 gcgcagtagt gacataaaca atgtcgtaag gttcaagctg gaattccgtt ccgagtatca
    14221 ttgccgatgc gtccttcgca ttcagctgat aaatattggc gattttgcca ttctgcttgt
    14281 tttgcgttgc acgaataaca aaaatccccg tcgcatcagc gacatcctga ttcataccct
    14341 cagcgttacc cagcgcttcc gccagggtca taccgctgcg gtccattttc agcgtgctct
    14401 gtttacccac ttcacccatc acgaagactt tcagatcatc gttgcgcgga ataaataaaa
    14461 tatcgcccgg gtgcagcagt ttgttctggc gcaagtcgcc acgctgcatc agcgcataga
    14521 ggttcacttt ggtctttacg ccattctggg tgagcaccac gttgcgccag tctgcatctg
    14581 cggtcagccc ccccgcggcg ttaatcgcat ccatgatggt cagaggaata ttggtaatcg
    14641 gctgctgacc cgatttgctg acttcaccgg tgacgtacgc tttttgcgag cggaacgccg
    14701 cgacgctgac atcgacctgc gggctttcaa tgactgaatc cagacgcgct gtaatctcgt
    14761 tgcgtacctg ggtaagcgtt ttcccggcca ctttcaggcg accaatatag gggtagaaaa
    14821 ttgcaccatc agcgttaacc cagttgccgg tatcactggc gctacggtat tgccctgcgg
    14881 gtgtggttag ttccgggtga tcccagaccg taaccatcaa cacatcacca atgccgatac
    14941 gatattcata gttcgcgatt tgctgctcca gctccgggtt agcctgcgac ataatcggtt
    15001 tggcgcgcag ctgatcaatc aatcctggcg tgacgggata gatattgacc attttgtcca
    15061 ggtcatactg attgtcaggt agttcaatga catccttgtt actggtcgat aaattctgac
    15121 caggaacaag ggtacatgct gtcaaagaca gaacagccaa tgtcagtacc gaaaacttaa
    15181 aaatgttctt tttcattggt ttatatttat cagtcattgt aatgttatga attacgtcag
    15241 gttattgtcc aaccggtact tctcgctctg ccgtcattgg atagagctgg tatttgcgtg
    15301 acagcatttg tccaccatca cgcgtcaatg gtgtccacga aaccaccgcg cggttgcggt
    15361 taggtccaat ggtcatcaag tcgaacggaa tcgagatata aaaaccttta ctaaagccgc
    15421 cttcgccgta gtcatctttt gatacgttac tgattgccgc ccatacccct actgccacgc
    15481 cgctgtcaaa gcgtttggcg acgtcgatcg ttgcaccttt atcttttgcc agatattgcc
    15541 caacgctaag tttcatcagt acgccattga gcgtcggcgg gttccagtaa gccgtcacga
    15601 aacctgttgg cgtagaataa tcggtgaagc gcatcatgtt gtcccagtca cgctgcttca
    15661 cgtagttaac gtccacaccc agcgcccagc tggcatctag cgggcgataa agcagctcgg
    15721 aaccgacacc ggcatacatc gtttccagat aaccgccata cacctggcca tagaaaccgt
    15781 tgcctaagtc ggcaaagtag ttcgcttgca agttgttgag ataaacgtca ttgcgaacgt
    15841 aatcacggat atgcgtgcgc acgcggggca gggtagagtc cgcgggtaat agcgaagact
    15901 taaacttgtc gtagttattg taaatattgg tgaaaatacc gccatcaagc agcaggtggt
    15961 cggtaaacca gtagcgggcg ctggacatca gccccagctg gaacatatag aaatcttccg
    16021 ggccgcccag cgactgtgaa agtgttgggt tgaaagagta gctaaagcga tcttcacgaa
    16081 tacggtagcc ccgaccaaag gcagaaagat cttcagcttc aacacgttgt tgttgcagtg
    16141 gctctgattg accaggcgct gtaccggcca gttgtttgcg caggctggct acgtcggttt
    16201 cggtagtcac catcgccata tgctcgcgct tttgcgtcac gctaatcttc tcaacgcctt
    16261 gcggcaggtt attcaccaga atccggttgg cacgatcgac cgcttcgcga gagtcacggt
    16321 atttgtattg ctgaccagac atatacagcg tcttatcgcg tagctgaatt tctggcgcgt
    16381 caaagcccgc gttatacttc agggcggtaa gttggtttgc taccgtggtg tactgcaatc
    16441 cttcagattc cggcgcaggt tgatatgccg gttttggcgt atcgcgcagg gcagggcgca
    16501 gatcgttgaa attggtccgt aacgtgaagc caaacatcaa cgtgttaccg cgttcataac
    16561 tcaggttgag atctgcccag ctggcagcgc gatagactgc gccgacgttg aaatggcttg
    16621 cctgaggcag tttgcccgca aaatcattct ggtaattgtt gccatcgtat tcgagtttca
    16681 gacgcagagg attccacggc gtttgatact caataccgcc aaaaatagag gctggaccac
    16741 gaaagatatc gctaaagctg atatctcccg catcgtgaga ctctgcgcga tggcaatatt
    16801 tatcgcttac ccgacaaaac gggttggtaa tattgcccgc attaccggcg tagccccagg
    16861 ccatcccgag ggtgaaatcg aacgggcccg cttgcttgct ggccaccaaa tattcaccgt
    16921 caaacagacc cgtaccagca atatcacgtt taccaaacgc cacttgcggt agccagtaac
    16981 cttcttccca caggcgaagt ttaaaatcga aagatttgtc tttgtatgac tgatcgccgc
    17041 tgaaatcttc ccactggcta tatttgcgtg tgcgcacatc cgtataacga atggtgcctt
    17101 ccagccatgg gaacagcgcc acggaggtgg agtagaaacg gtattgatcg ttatcccggt
    17161 agtttacgct gaattcacct tccggtgcga tgcgcgcatt tggcatctgc aacaatcctg
    17221 tgccgccaaa gtctgattgc gacgaaccca gcggatccgg ataggttaat acttcagcat
    17281 gacaggcact ggagacggca atggccaggc agcttaaaag ataagagttc ttcttcatca
    17341 gtcaggaaca cgttgcgtta aaacagacac aatctggtca tttaaatcag catatttttc
    17401 gggcagtaca tgcgcagaaa agcctaacca aagctggctg ccaggcggtg gttccacatg
    17461 tcttttattc cacagggcaa caggggcgac gacagcctca ccctcaggcg taatcaccat
    17521 aacgttattc ttgtccgcgc ctgcaagacg ggtgtgatct tgcagataat cggtaacgct
    17581 acggcctgcc agccagggca attgcccagc cccagaaacc gcacctagca gagtaatagt
    17641 caccggacgc tgtacggtat agagcgtata gtccccgacc agcgggggat tgctgttctc
    17701 atccactcgt acaaaatcag ggtccagttt gaccggtaag cgaccagtga tattgagatt
    17761 gagtaactgc tggcgcacgg atttaatcgt tgccaccaca tcgtcgtcag cttctgcttc
    17821 ccaggaagca agctgtgcca tgacatgttg gtagtcttta agcgctttag ctttcgccgc
    17881 agagtcggtc agcaaggcac caggccacca gaggcgatcg cgcagttgcg gttgtgtcac
    17941 caactggaca acattttcca cggggccaac ggaaagtgtc tgttgttcgc caggcagata
    18001 aatagtcacc gttccttgcg caaaggcatg gggtgtcatt acgtaaagta cgctggcaat
    18061 gaaatacgac tgtaatttat tcatggtgcg ggtttaagaa acgtcatttc tacgggaata
    18121 acccctgcgc ctaacatttg ccgactttga cgaacctggc cagtagccga atcgacccag
    18181 aatgtgtttt gccactgttt ttctggcagc gtggaagtca cctcttcctg ccagacatta
    18241 cacagcacat cgcgacccgc aatattcagc gtctctaatc cagcaaagga aaaagtggaa
    18301 ctcagggtcg cagagcgaaa atggttgtct tcactccaga gaatatcgcg cgtccagcgg
    18361 ctgccgtctt ttattgccag cgcgttacgc agagggtcct gcccggaatt tgtcacttcc
    18421 agcagattat tattaagttt gacggttttt aaaagacgtc cattgtgggt aaccagcatg
    18481 gcgttatcct gggataacca tttgctttgt tcttgttcta tatatcccag taccacaaag
    18541 attcgttgcc cttcattaag gcgtaaatac atcgtggaat aaggcaacgc ctggatctgc
    18601 tgatccgcta cggtgatatc ctgattatcg aaaaggctgg cgcgaaatgt atcgaccata
    18661 ctttgctggc tgtgcgtaca tcccgcaaga aatagtgcga aaatcgataa aataagaggg
    18721 cgcaccttta atccttgaaa ggttaattaa aaaaatgcaa atctctaaag cccggtacga
    18781 atatcgcaac cgggtatcat ttaactttaa ttttgacgcg ttaaagcagt attagcgcgt
    18841 agtggacgtg gtggttgagg tgttatgaga accgccatcg ccactgtcat ttgctgcaac
    18901 tactgccaac gtcgccacac caacagccgt tgccacaccg acaaccgtcg ccgtgccgac
    18961 agcgccagca gccacgccac cagtggtagc agcagtcgtg gttgcacctg tagtggtcgt
    19021 tgtggtgcca gtagtacccg tcgttgcctc agtcccgttt gctgcttcag gggcagcaaa
    19081 tgcagctggc gtcgtcaaca tgaaggccat aaggattgca gaaagcttgt gtttcataac
    19141 ttttccttta ttcatcgcat ggacaatacg tacgcttgag atcaggatta tctactttga
    19201 gtataacatc cttgtgaaat ctccctttcc tcataccgcc agcactatat actgcgggtg
    19261 ggaaaggcgc gcagagggaa atataagatt gttaactatt cccctctgga aatacgactt
    19321 gtgagcataa gataaatctt agcaaaatac aaattaaaga taagaaaatt gttaagaaag
    19381 ctattaacta ttgttggtaa tatagtttca agtggtacta taactggctg ttaagtggca
    19441 tttataacta ttaggtgcag agatattcgc ttaaatggat ttgttgctcg gtgaaaaagt
    19501 aacttgcgtt ataatttatg gtgttgacta ttgagctatt ccaataaaac cagccaactt
    19561 taaacaagca actcatatta agagagataa acatttgccg ctgttggtcc tcgcaggcca
    19621 tttacgcggc aaaattccac gcgtaatcct ggtataagca cttctgcgtc gcggggagtg
    19681 aatgcggaaa tatggacctg gacttcttta cgaccgtcgg aggggataat gaatcctttg
    19741 ccgcttttgc gatcaaaggt tttgacaatt cctgtcattt tacgggacaa acaaattcct
    19801 tactgaaaat actgcgctgc actatacggg gttaataaaa taaagccagc gatatttaag
    19861 accgccggac ggctaaaata aaatttgctt aatctcaatt atcatgcgtt aatagctgcg
    19921 tcggtttgaa agacagacag catacaaagt agtttactaa agcagttctc attatcaggc
    19981 attatcccct tcttttgagt ctctctcctg aacactaagt agtttctgta ctaaagccct
    20041 gtttgccgaa aggcccaaaa tgaaggaagt aaaatatgtc taataaaatg actggtttag
    20101 taaaatggtt taacgcagat aaaggttttg gctttatcac tcctgatgat ggcagcaaag
    20161 acgttttcgt ccatttcacc gccatccaga gcaatgaatt ccgcacgctg aacgaaaatc
    20221 agaaagttga attttctatt gagcaggggc aacgtggtcc cgcggcagcg aacgttgtta
    20281 cgctctaagg ttgccgttat tactccacat ctccatttcc gctgtccatg ttgtcatggt
    20341 tcacagtacc gcacatcggc attcgatgtg acggagcgaa atcctttagg ggcaaagtgt
    20401 attttttgta aatcgacgat gatcaccttt gataacgtcg cgctgcaaat acgcactgac
    20461 catgcgccgc tggatttcac aaaataatat caggctccct cgtggagcct tttttatatc
    20521 tgccttattt ttcttcaacg ctgtatgtat agtaagcgat aacctgttga ttattgaatc
    20581 tttcggggag atggcttata acatttctta cctgaccagg gtaccgggaa ccaacacctt
    20641 actggcgtgt tgctgtcttt caagaccaga agaggttaac agtgaatatt gaagagttaa
    20701 aaaaacaagc cgaaacggaa atcgctgact ttatcgcgca aaaaatcgcc gagctgaaca
    20761 agaatacagg gaaagaagtc tctgaaattc gcttcaccgc acgagaaaaa atgaccgggc
    20821 ttgaaagtta tgatgtcaaa atcaaaataa tgtgattttg tgaacatcac ccgtgcgagg
    20881 tgatgttccg cttgttgcta atttagtggc caatcattgg cgcttgtgga attaagcgtc
    20941 ggtacaattc ctccggcacc gggctttgcc atactcccgc atacattgcg taaccaatca
    21001 ccgcaaacat aatccccaga atcagtagcg tcattaacca gccagacaac gcaaaggctt
    21061 ttttatttgc cgcaggtttt tgcagtgaaa aggtcaatgt tgaggctacc ggacatgact
    21121 ctacgcaagt catacagccg gtacattcca ctgttcgtac ctgaattaat ttatcgaccg
    21181 ggatccgtga tgggcaattt tttgcgcatt tgccacagtc gatacaactt tcggcattgc
    21241 gacgaatctt aaacggcgat aatagcgaaa ccacgcccat cagcgcgcca tatgggcaaa
    21301 gataacgaca ccaggcatgg cgaataaaca ggctggcaat cagcaaaacg atcatgctga
    21361 ttaatgtcgc ggtccccata tgacgaaaga aattgagcat tttaacgtcc atcaccacgc
    21421 tgtagggcga caacataaaa tagtgaatcg cctgagcggg catcaataac gcaatataga
    21481 gaaaaaaact caacagcaaa tacttcacgc cgcgcagagg agtatccagc cagcggggaa
    21541 ggacaaattg ccgaccaaac agtttgttac cgagatcgcc gattaattca gaaagcgtac
    21601 caaccgggca taaccatgaa caaaaggcct tttttaataa tagactgatg acgataaaag
    21661 cgaccaataa cagcatcgcg gcggcgtgga cggacggtaa ctggcctgtt gcaaggctat
    21721 atttcagatt catcagaccg gcaatcggta gccagccttc gatacctccc ggtctggtga
    21781 caaatgtcgt actacctgcc gtttcgtaat agcgcaccca ataccagaac gtgatggcaa
    21841 tataaatatt cattgccaac agtaataatt gcgtcgcttt acgccaggtc gtggcattgc
    21901 gccagtcatt ccacggtaat ttgccgcccg tcgtgcctgg ccgccgctgc cagcgggttc
    21961 ttcttttctc tgtcatgatt ttgccagtcc gttaagttgc ataccaaatg ccactattct
    22021 agttgttctt aactggctga tattgattca aatcgcgttc aggtctttct tatgcaacca
    22081 tgcttccaga gcggcaacac tgcgtgtaat ttcttcgtgt ggaagggggg cagataatgg
    22141 ctgctgctcc agttgtgcgc atagctggct ggcgatacgc attcccaggc tcgagcaact
    22201 gctttttagc tgatgtgcgg cacgctttat tttctcgcta tcctgattgg cgcgggcaat
    22261 gtcgatgtca tcgagaagcg gcagggcatg ttgtgtaaat aataccagcc attcgtggat
    22321 cttctccgtc cccattaact gagcatcttc attgagttgc gatacatcca gcggttgatc
    22381 gttattgact tgcagttgga gatagtgcgc cagtaactga ccgagcactt cacgcggcac
    22441 cggtttaggg ataatcccgc gaaatagcga actggtacgc tggcgcaacg tttcgtcgat
    22501 gacatgggcg ctaaagccaa tcaaaaccag cgacggatat tgctgtgcca gttgccgggc
    22561 aagcgtaatg ccgtcgatat ccggcagatc aaaatccacc agtgcgacag caaatggttc
    22621 actattttgc agtgtctcta aagcctgcgc ggcatttcct acggcaacga cctgcgcccc
    22681 actgctgttc aacatctcaa cggtaatccg ctgggttagc gggttatctt caattaacag
    22741 caaacgtaaa ccgtccagac gcaccgcctg attgactgtt tttggcacgg gtgcagtggc
    22801 aatacgtaac agcaagcgta aacaaaaaca gcttccaacc tccggcgtgc tggtggcgct
    22861 cagttcgcca cccatcgcct gggccagacg gctactgata gtcagcccca gcccggtgcc
    22921 gccgcgtttg ccgctaacct gaacaaatgg ctggaagatt tcagccagtt tcgcgggatc
    22981 aataccgcag ccgctgtctt ccacttcgac cagccattgt tcgccatcag tgcgactacg
    23041 caggataatg tgcccttcgt cggtgaaacg cagggcgttg ctcaacaggt tggtaatgac
    23101 ctgacgaata cgctgcggat cgcccattaa cgcagtcggt acatcgtcgg caattgccgt
    23161 tgccaggcga atcgggcgac ctttcacccg tccgctcatt aattgcaggg tactttccag
    23221 caacgggcgc ggttcaaagg gttcatcgct gaccgaaaca ttcttgccgc ctgcttcgat
    23281 agccgaataa tcgagaatat cgttgaggat ggtcagcaac gattcgccag agtcggtaat
    23341 tgcccgcaaa tcatcacgct gagcgttaag tgcggggtta tctgccagta gctgtgcggt
    23401 accgagaata ccgtacagcg gtgtgcggat ctcatggctc atcgccgcca gaaacgctga
    23461 ttttgcctgg ttggcttttt ctgcttccgc ccgtgcctgt cggtgttcta tcaccagttc
    23521 ctgcaattca gctgtacgcg ctttgacctg cgccgccaac tgttcacggt ggcgattcag
    23581 tgcatgaaca ctgctgcgaa acgcatccat cagccgcccg atggtgtcca gttcccgcac
    23641 gccagcggtt tccgggaaag gggagtcgat atcaccatcc agcaagcgtt gtagcgcctg
    23701 cgtttgttct gcaagagggc gcgtgactga gcgataaacc acgcgccaga ggatcagaat
    23761 cagtgcgcaa agagaaacta ttcccagcaa taacaggctg tattgcccgc gtgcactggc
    23821 tttttccaga tgtgccagcc cgtgctgatt acgcagctca atggtgtcga ccagctgact
    23881 gacttcgcta ctaaactgcg cgaattgggc gatgttattt tgggcgagag tttgcaggtg
    23941 attgctgatt tcactgtcct gctgatacag cgccagcaaa tcgctatatt ggctaacggt
    24001 agttaacgtt gttgctacct gagtacgaac acccggatct tcaatgcgta tttgccgacg
    24061 ttgcagaatt ttcaccgcat tattgagctg cttttccagc gttggcgcat ttttctggat
    24121 ctgttccagc cccagattca tcaccatttg ctgcacccgc agggcgctaa ggcgcagttc
    24181 attcatctgg ttaacatact caagatcgat atcaatcagc cgatcgagtg cactttctgc
    24241 ggcctgacgc tgatgttgtt cgatcaaatc gtaaatcccg gcctgggtcg ctccggcgga
    24301 ggttgccgca ttattcgcct gaccttgcgc caggcgtgcg atttcatcgg cggcggcgac
    24361 tatctgctga ctgagttgct gttgttgctg gcgaagttgc aaacgctgcc ccaccagttc
    24421 cccttgctga cgtaacgaac gggagatctc ctgctcctgt tgttcaatag cggtggtgtc
    24481 aaaatcttgt tcccgtaacg cttgcagcaa cgcattaatc ttcaggcttt gtgcggtgag
    24541 cattcgcccc tgcgcctgcc acatcttttc gttatcggca ctggtcaggt tctgcgcggc
    24601 gaaaagttcc caggcgctgg cttcgctcaa ctggcgcgcc atattcatgg taggaatcaa
    24661 tgcctgagtg ttgtcttttt ccacctggct gataaagcgc aggttgtacc atcccaccag
    24721 ggtactggtc agggttaaca gcgccatcag ggcaaagccc atccagagtc ttcgggtcag
    24781 ggttaaattc acggtcggtg cactttgggt gaaaaaggtt gagtcgcaaa gcggaatgca
    24841 tctagcataa agccttatta ttgatgaggc tatcatgcgc gtactgctat tgttactact
    24901 ttcccttttc atgttatcgg cattttcggc tgataacctg ttgcgctggc atgatgcaca
    24961 gcattacacg gtgcaagcct ctacgccgct aaaagccaaa cgcgcatgga aactgtgcgc
    25021 gctttatccc agtttgaaag attcatactg gttatcgttg aactatggta tgcaggaggc
    25081 tgctcgccgc tacggtgtgg atttaaaagt gctggaggca ggcggctaca gccagttggc
    25141 tacccagcaa gcacaaatcg accagtgtaa acagtggggc gcagaggcca ttttgctcgg
    25201 tagtagcacg acgtcatttc ccgatctgca aaagcaggta gcaaatctgc cggtgatcga
    25261 actggtaaat gctattgatg ctccccaggt gaaaagccgc gttggtgtgc cctggtttca
    25321 gatgggctat caacctgggc gatatctggt gcaatgggcg cacggtaacc cactgaacgt
    25381 gctgttgatg cccggacccg ataacgccgg gggcagtaaa gagatggccg agggttttcg
    25441 cgcagctatt gctggaagcc cggtacgtat tgttgatatt gcgcttggcg ataacgatat
    25501 tgaaatccag cgtaacctgt tgcaggagat gctggagcgc catccagaaa tcgacgtcgt
    25561 tgccggaacg gccattgcgg cagaggcggc aatgggtgaa gggcgtaacc tgaaaacgcc
    25621 gcttaccgtg gtgtcgtttt atctttcaca tcaggtgtat cgcgggctga agcggggaag
    25681 agtgattatg gctgccagcg atcagatggt ctggcagggg gaactggcgg ttgagcaggc
    25741 catcaggcaa ttacaggagc aatcggtgtc tgataatgtc agcccaccga ttttagttct
    25801 gacgccgaaa aatgccgacc gcgaacatat ccgccgctcg ctgtcaccgg ggggatttcg
    25861 tccggtctat ttttatcagc acacatcagc ggctaagaaa taaccttcgc catgttgcgt
    25921 caccagtaaa tccgcgctga gtttatggcg taaacgacga attaacacat cgacggtgcg
    25981 caagtcaggg ttttccaccc gacgcgcaga gagcatacgc agcagacgtt cacggctaag
    26041 aatttcgccc ggattcgtca caaatgccac caacatttca tactctgcgc gggtcagttt
    26101 aatcggctcg ccatcccgct ccagcgtatg gcgcgacaca ttcaggcaat aaccggcaaa
    26161 gcgatagcag ttgtcctgtg tgtgcggttg agcttgtcgc gcgaggtcga ttcgccagag
    26221 cagatttttc acccgtacta ccagttcgcg cagttccagc ggtttggtga cgtaatcgtc
    26281 tgcgcccatt tccagcccaa caatacggtc aatccgatcg ctgcgtcccg taaccagaat
    26341 aatccccacc gttgagcgtt ctcgcagggc gcgggttaac atcaggccat tttcatcggg
    26401 caaattaata tccagcagaa ttaaatccac cggctgattc tgcataattt cccgcagccc
    26461 ggcaccgctc gccgtaacgg aaacggtata cccctcctga gtgaagtagg attgtaatcg
    26521 cgcctgggta accggctcat cttcaacaat aacaatgtga tgtggcatca gagggtttta
    26581 ctcattctgt tcatatctgt tcatattctg ccgtaagccg ttcatcctga ccagtgccgc
    26641 tgttcatatt tactcattaa gatcgcttca ctaaaccata attttacagg ggctattatg
    26701 cggaaactct ggaacgcgct acgccgaccc agtgctcgtt ggtcggtact ggcgctggtc
    26761 gctattggga ttgtgattgg cattgcgctg attgtattgc cacacgttgg gatcaaagtc
    26821 accagcacaa ccgaattttg tgtcagttgc cacagtatgc aaccggtgta tgaagaatat
    26881 aaacagtcgg tgcacttcca gaacgcctcc ggcgtgcgag ctgaatgcca tgactgtcat
    26941 atcccgccgg atattccagg catggtgaag cgcaaactgg aagcgagcaa cgacatttac
    27001 cagaccttta ttgcccactc cattgataca cctgaaaaat tcgaagccaa acgcgcggaa
    27061 cttgccgagc gtgaatgggc gcgaatgaaa gaaaacaact cggcaacctg tcgctcctgc
    27121 cataactacg atgcgatgga tcatgccaag cagcatcctg aagcggcgcg tcagatgaag
    27181 gtggcagcga aagataatca atcctgcatc gactgtcata aaggtattgc ccaccagtta
    27241 ccggatatga gtagtggctt ccgtaagcag ttcgatgagc tgcgcgccag tgctaatgat
    27301 agtggtgaca cgctgtactc cattgatatt aagccgattt atgcggcgaa aggcgataaa
    27361 gaagcgtctg gttctctgct gcctgcttcg gaagtgaaag tccttaaacg tgacggcgac
    27421 tggctgcaaa ttgaaatcac tggttggacg gaaagtgcag gacgtcagcg tgtactcacc
    27481 caattcccag gtaaacgcat ctttgttgcc tcgattcgtg gtgatgtgca gcagcaggtg
    27541 aaaacgctgg agaaaaccac cgttgccgac accaataccg agtggagcaa gttgcaggct
    27601 actgcgtgga tgaagaaagg cgacatggtg aacgatatca aaccgatctg ggcttatgcg
    27661 gattcgctgt acaacggcac ctgtaaccag tgccacggcg caccggaaat ctctcacttt
    27721 gacgctaacg gttggatcgg cacgctcaac ggcatgattg gctttaccag cctcgataaa
    27781 cgtgaagaac gcaccttgtt gaaatatctg caaatgaatg cgtctgacac cgcaggtaag
    27841 gctcacggcg ataagaagga agaaaaataa tgaacaataa cgatctcttt caggcatcac
    27901 gtcggcgttt tctggcacaa ctcggcggct taaccgtcgc cgggatgctg gggccgtcat
    27961 tgttaacgcc gcgccgtgca actgcggcgc aagcggcgac tgaggctgtc atctcgaaag
    28021 agggcattct taccgggtcg cactgggggg ctatccgcgc gacggtgaag gagggtcgct
    28081 tcgtggcagc aaaaccgttc gaactggata aatatccgtc gaaaatgatc gccggattgc
    28141 cggatcatgt acacaacgcg gcgcgtattc gttatccgat ggtacgcgtg gactggctac
    28201 gtaagcgcca tctgagcgac acctcccagc gcggtgataa ccgttttgtg cgcgtgagct
    28261 gggatgaagc cctcgacatg ttctatgaag aactggaacg cgtacagaaa actcacgggc
    28321 cgagtgcctt gctgaccgcc agtggttggc aatcgacggg gatgttccat aacgcttcgg
    28381 ggatgctggc gaaagcgatt gccttgcatg gtaatagcgt gggtacaggc ggagattact
    28441 ctaccggggc tgcgcaggtg atcctgccgc gcgtagtcgg ttcgatggaa gtgtatgaac
    28501 agcaaacctc ctggccgctg gtattgcaga acagcaaaac cattgtgctg tggggctccg
    28561 atttgctgaa aaaccagcaa gcgaactggt ggtgcccgga tcacgatgtt tatgaatatt
    28621 acgagcagct aaaagcgaaa gtcgccgccg gtgaaattga ggtcatcagc atcgatccgg
    28681 ttgtcacatc cacccatgag tatctggggc gcgagcatgt gaagcacatt gcggttaacc
    28741 cgcaaactga cgtgccgctg caactggctc tggcgcatac gctgtacagt gaaaacctgt
    28801 acgacaaaaa cttcctcgct aactactgtg taggttttga gcagttcctg ccgtatctgc
    28861 tgggtgagaa agacggtcag ccgaaagatg ccgcatgggc tgaaaaactg acaggtatta
    28921 atgccgaaac cattcgtggg ctggcgcggc agatggcggc gaacagaacg cagattattg
    28981 ctggctggtg cgtgcagcgt atgcagcacg gtgaacagtg ggcgtggatg attgtcgttc
    29041 tggcggcgat gttggggcaa attggcctgc caggtggagg ttttggtttt ggctggcact
    29101 ataacggcgc aggcacgccg gggcgtaaag gcgttattct gagtggtttc tccggctcta
    29161 cgtcgattcc gcctgttcac gacaacagcg actacaaagg ctacagcagc accattccga
    29221 ttgcccgttt tatcgatgcg atcctcgaac cggggaaagt gatcaactgg aacggtaaat
    29281 cggtaaaact accgccgctg aaaatgtgta tttttgccgg aactaacccg ttccatcgcc
    29341 atcagcagat caaccgcatt attgaaggct ggcgcaagct ggaaacggtt atcgccatag
    29401 ataaccagtg gacctcaacc tgccgctttg ccgatatcgt gctgcctgcg accacgcagt
    29461 ttgagcgtaa cgatctcgac cagtacggca accactccaa tcgtggcatt atcgccatga
    29521 aacaggtcgt gccgccgcag ttcgaggcgc gcaacgactt cgatattttc cgcgagctgt
    29581 gccgtcgctt taatcgcgaa gaagccttta ccgaagggct ggacgaaatg ggctggctga
    29641 aacgcatctg gcaggaaggt gtacaacagg gcaaaggacg cggcgttcat ctgccagcgt
    29701 ttgatgactt ctggaataat aaagagtacg tcgagtttga ccatccgcag atgtttgttc
    29761 gccaccaggc attccgcgaa gatccggatc tcgaaccgct gggcacgccg agtggcctga
    29821 ttgagatcta ctcgaaaacc atcgctgata tgaactacga cgattgtcag gggcatccga
    29881 tgtggtttga gaaaatcgaa cgctcccacg gtgggcccgg ctcgcagaag tatccgttgc
    29941 atctgcaatc tgtgcatccg gatttccgac ttcactcgca gttatgtgag tcggaaacgc
    30001 tgcgtcagca atatacggta gcgggcaaag agccagtgtt cattaacccg caggatgcca
    30061 gcgcgcgcgg tattcgtaac ggtgatgtgg tacgcgtgtt taacgctcgc ggtcaggtgt
    30121 tggcaggggc agtggtttct gaccgctatg cacccggcgt ggcgcgaatt catgaagggg
    30181 catggtacga tccagataaa ggcggcgagc ctggtgcgct gtgcaaatac ggtaacccca
    30241 acgtgttgac catcgacatc ggtacttcgc agttggcgca ggcgaccagt gcgcatacca
    30301 cgctggtgga aattgagaag tacaacggaa cagtggagca ggtgacggcg tttaacggcc
    30361 ctgttgagat ggtggcgcag tgcgaatatg ttcccgcgtc gcaggtgaaa tcatgaccac
    30421 gctgacagca caacagattg cctgtgttta cgcctggctg gcgcagttgt tctcccgtga
    30481 actggacgat gaacaactga cgcaaatcgc cagtgcgcag atggctgaat ggttttcgtt
    30541 gctgaaaagc gaaccgccgc tcactgcggc ggtgaatgag ctggaaaacc gtattgccac
    30601 tcagacagta cgtgacgatg ctcgtctgga actggccgct gacttttgcg gcctgttttt
    30661 gatgaccgac aaacaagctg cgctgccgta tgcatcggcc tacaaacagg acgagcaaga
    30721 gattaaacgc ttgttagttg aggcagggat ggaaaccagc ggcaatttca acgaaccggc
    30781 agaccatctg gcgatctatc tcgagttgtt gagtcatctg catttttcgc tgggagaggg
    30841 gaccgttcct gcgcgaagaa tcgacaatct gcggcaaaaa acactgacgg cgctgcgaca
    30901 atggttacca gagtttgctg cgcgttgcca tcagtacgac agtttcggtt tttacgcggc
    30961 actaagccag ttattgctgg tgttagtgga atgcgaccac caaaacagat aacgtcgttt
    31021 gttggcctga taagacgcgt ttagcgtcgc atcaggcatt agtgcgcagt tgccggatgc
    31081 ggcgagaacg ccttatccgg cctacgggaa cagtgctttt tgtgggcctg ataagatgcg
    31141 ttaagcgtcg catcaggcat tatggcgcag ttgccggatg cggcgagaac gccttatccg
    31201 gcctacggga acagtgcttt ttgtgggcct gataagacgc gttaagcgtc gcatcaggca
    31261 acaaactcac gggtgagcca caaaccggga aagccgctgg cgcagcaggc ggttttcctg
    31321 cttcaggtgc gcaatatcat ccattaacgt cagcgccacc gcgatccccg gccagtccag
    31381 agccagttca tgacgcaggc gtaccgcgcg ttgcaccaca atggcggcat ggtcgtcaaa
    31441 tacccaggtt gtctcctgga tctcccgcgg ttcaaccacc cccaaaccga caatttcatt
    31501 caactcctct tcagagatgc cggtatgcag gcaaaattcg gtaatagtaa aagtcaccgt
    31561 aacattagcc attatgcttt cccccaatct ttacgtggat caaaagacga ctgggcgtct
    31621 gccagttgct gccacagcgc ggcagtgttt tcatccggtt tcggcggcat cacgattttc
    31681 agtaccgcat acagatcgcc ggtctgtttt ttgctcacca gacctttgcc tttaacgcgc
    31741 aatcgttgcc cggcctggct gccaggcggg atagtcagca aaatgctttc tttcagtgtt
    31801 ggaacggtga ctttagtacc cagcgccgct tcccacgggc taaccggcac cactatttcc
    31861 agatcctggc cgacaatatc aaacagcgga tgtggcgcaa tgtgaatcac cagccacagg
    31921 tcgccatttg gaccgccgtt ttcgcccggc gtcccctggc ctttcaggcg gatgcgttga
    31981 ccattgccga cgcccgccgg gatcttcaca ttcagcgttt tcgggatttc ctgttcgatc
    32041 atgccaaagg cgttataaac cggcaggtta tagctgatgg tacgcttatg ctcagtaagc
    32101 gtttcttcga ggaaaaccgc cacttcgatt tcaatatcgt ggccgcgtgt ggcggggcgt
    32161 tgacggctct ggcgggcatg ctgaccgaaa attgacgaga agatatcgtc aaaatcttcg
    32221 gcgttaaaac tctgaccgtc gccatgatgg aactgacggt taaattgcgg atcgttgcga
    32281 tgttgccaca tctgatcata ctcagcgcga cgttgttcat cacttaacac ttcccaggct
    32341 tcggcgacct ctttgaagcg ggcttcggca tccggttctt tgctgacatc aggatggtat
    32401 ttgcgggcca gtcgacgata ggcggtcttg attgtcttga gatcgtccgt cggtttcacg
    32461 cccatgatgg cgtaataatc ctttaattcc atagcgttat ctcgcgtaaa tcaacacaaa
    32521 ttgaaggaac ccctgtaagg taactcctat aagtgtaggg taatcctcaa aatttcatat
    32581 gccaacacag aatatgttat tgaaatcatc gcggagggga ggtcgcctca aggtgggagg
    32641 aacatatttt atttacctcc ctcctgaact tttggctcat gcttacaagt taaacaataa
    32701 ggaaaggaat gcacaactcg actatttaaa tcgttatgca atttaatgat aaagacatgt
    32761 gctaaaaacc atttctatta gtctcacatt ctttttttat tggtaatggt aacgccgctg
    32821 attttcttta tagctactga tgtaatcatt agcggcgtaa acgaggccgg ggctaatcag
    32881 gcatagtttg cgtcaaaact tgcctgtttt taaaggagaa tatagaaaaa caggcgttca
    32941 acgagccatt ttgcgaacct gttcccggaa aaatgacata tttctgtcac actctttaat
    33001 tattgataac aaaagaggtg tgaggaatga acaaaacgct aatcgccgca actgtggcag
    33061 ggatagtttt actcgcttca aatgctcagg cacaaaccgt accggaaggc tatcagctac
    33121 agcaagtcct tatgatgagc cgtcataact tacgtgcgcc gctggcgaac aatggcagtg
    33181 tgctggagca gtcgacgccg aataaatggc ctgaatggga cgtccccggt gggcagctca
    33241 ccactaaagg gggcgtgctc gaagtgtata tgggccatta tatgcgtgaa tggctggcag
    33301 aacaggggat ggtgaaatca ggggaatgcc cgccaccgga caccgtttat gcctatgcca
    33361 atagtctgca acgcaccgtc gcgaccgcgc agttctttat tactggcgca ttccctgggt
    33421 gtgatattcc tgttcatcac caggaaaaaa tgggcactat ggaccccacc tttaacccag
    33481 tgatcaccga tgattccgcc gcattcagtg aacaggcggt ggcggcaatg gagaaagagc
    33541 tcagcaaact ccagcttacc gacagctaca agctactgga acaaatcgtt aactataaag
    33601 attctcctgc gtgtaaagag aagcaacaat gttcgcttgt agatggcaaa aataccttta
    33661 gcgcgaagta tcaacaagaa ccgggcgttt ccgggccgct gaaagtcggc aactcgctgg
    33721 tagatgcgtt tactttgcaa tactacgaag gttttccgat ggatcaggtg gcctggggag
    33781 aaatcaaatc tgaccagcag tggaaggtgt tgtcgaagct gaaaaacggt taccaggaca
    33841 gcctgtttac ctcaccggaa gtggcgcgca atgttgcgaa accgctggtc agttatatcg
    33901 acaaagccct ggtcaccgat cgcaccagtg caccgaaaat cacggtatta gttgggcacg
    33961 actccaacat tgcctctctg ttaacggcgc tggatttcaa accgtatcag ttgcatgacc
    34021 agaacgaacg cacgccgatt ggcggcaaaa tcgttttcca gcgttggcat gacagcaaag
    34081 ccaatcgcga tttgatgaaa attgaatatg tgtaccagag tgcggaacag ttacgtaatg
    34141 ccgatgcgtt aaccctgcag gcacctgcgc agcgcgtgac gctggaatta agcggttgcc
    34201 cgatagatgc caacggtttt tgcccaatgg ataagtttga tcgcgtattg aatgaagcgg
    34261 tgaaataaca gaaaactccc ccgcgtgaag cgggggagtc gctggttaaa cgtttttacg
    34321 ttcgatggtc tgttcgcccc aaaaaagcga atctttatcg gtcttagcaa aggctttgac
    34381 taacacttca tcgctacctt cttcccaaat cttttccgcc attttttcgt cgtacccggc
    34441 gacttcgaaa atggcctcgg ctatttccgg cgacgtattg cgcagagatg cccattcacc
    34501 gacgtgatga gctttcgctt cttgagttgg catgcgtatc ctcctgttga agattagctg
    34561 ttaagtttaa ctgccagacc tgcgacatat tccccttgat aacgagcaat agacagttct
    34621 tcctggctgg gctggcgtga accgtcaccg cctgcgatgg tggttgcgcc gtacggcgta
    34681 ccgccgcgaa cctgtgaaac gtcaaataat tcctgcgctg cgtagccaat ggggacaatc
    34741 accatgccgt gatgcgcaag ggtcgtccag gtggatgtga tggtttgttc ctgaccgccg
    34801 ccagtaccgg tggaactaaa gacgctcgcc agttttccgt atagtgcgcc ggaagcccac
    34861 aggccgcccg tctggtcgag gaaggtacgc atttgaccgg acatgttacc aaagcgggta
    34921 ggtgtaccaa aaataatggc gtcgtaatcg gccagttctt gcggggttgc aaccggtgca
    34981 gtttgcgttt taccgcctgc tttttcaaat aattgcggcg gcatggtttc cggtacacgc
    35041 ttaacgacaa cttctgcgcc atccactttg cttgcaccct cagcaactgc gcgtgccatc
    35101 gtttcaacat gtctaaggat atggtttatc attttgaaaa atataatttt atttcatcct
    35161 cctgatcact ttggggcacg tctggggcgc gggaattaag gatattattc aacatagcta
    35221 cctgagtcac gctgcactca ggcatccatg caccataaac attgtagacc atgctggcgc
    35281 tggagtgccc catctgtgac gcaataaatg tcgggtttgc tccggcagat aaagcccagc
    35341 acgcataggt atggcgtgac tgatacgctt tacgggatcg gatacccgct ctttttattg
    35401 ctgaatccca tgtcgctccg atggagctta ccgcgtagtt aatacccgcc ttgtgattct
    35461 tgcgaacgat ttgcggacag aaaacaaaag tgcactcgtg caaaattgtt cttccgtact
    35521 cgcgtaattt aacagtgatc tgatgctgcc tgccaagacg agtaagcatc gcctggtttt
    35581 taagtgcttc aattgctggt gccagaagat gtataacccg gttagtgcct gcgtcagtct
    35641 ttggtagcgt aaaatcacct atttttgtaa aatttcttcg cactgttatc gtgccagctt
    35701 tcaggtcgat atcctcccat gcaagtgcgg caatttcacc gtgtcgcatc cctgtaaaaa
    35761 cagccactgt ccagaggttt ttggtctgtt gatgatggca ggcatcaatg agacggctaa
    35821 actcatctct ggtcagtgga tccggcactg gttttgattt cctcagcggt gttattgaat
    35881 taaacgggtt tttctccaga tacccgtttt cggcggcaaa gctgaacatt ccggctgttg
    35941 ttgtcatgta atagttcact gtgggtacgg ttcttccttt ttgggacgtg ctggttttcc
    36001 tgcttccctt ttccccggtc agtaaatctt tcctgataaa cagcagatct tcttttgtaa
    36061 tcgatgacgc cagcctgcca ggaccaagtc tcggtagcat atttttcatc actgattgat
    36121 aacgatttaa ggcgttacta ctgatttcca ttgctttcag agtaagccat ttctgtgcca
    36181 gttcaccgac ggtgatatct ttttttacca ggccaaatag cttcaggtta ggtgagtcag
    36241 ggaatcgctc ggcataatca aacgttcccg ttctgattgc aaagcagaca gaagccctta
    36301 actcaccagc gatctttctg tttttcggag tatcgggcac gcgaagattt tcgcgcactc
    36361 gtttacccct gtacttaaac gttattcgga gttttcctcc gtgattttca acgccggctg
    36421 gataggctga attcgccatt gttcctcctg cgtccaagag cacgctcagg ttatactctt
    36481 atggacaaga attaaacatc ctgagatggt aagggttggt ttttgatcca agtgttgatg
    36541 gtaggaagat gatagagaca ttcgctgttt ttctttggcg ttccgtcggg ggcaatttgt
    36601 ttatattctc tgccgttcat ccacgcactg tccctggcgc gtaatattgt tccttttctt
    36661 aggcctgtta cagccattaa aagctccagt gttacccatt cattcgcatt tatctgtaca
    36721 acaggtgtga tgactggctg aatgccatgg ttatgttggt ttactaactc gcgcataagt
    36781 tgcctctgca tgtgtaagaa aaaaccgccc gaaggcggtt gtcagttgat tgatgtacgg
    36841 cgcatttttc gaaggctggc aatatgcttt tccttctcaa tttccgcttt aatcatatgc
    36901 agttcgttgt gctcgattcg ctcaaattct tcattaaatg cactaattga agtggctctg
    36961 gttctgccgt cgagccttcg gtagatcacc tgagtcagag ttaccttgca tatttctacc
    37021 ggatagttgt tggcatcaac gaaagactgc ccgcgctgga tcaggacgaa cacggtttgc
    37081 actccctgga tttccgctac gttcactctg ctaattcgtt gatcaggtca cgatatttat
    37141 taagttctcg caacgcttta catgcctctt cccaacgttt ctgtttccgt ccggcgcggc
    37201 gtgcttcttt ttgtgatttg cgcagcaggt cacctacaat gttg
//
LOCUS       DAZGQL010000047        36526 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.047, whole genome shotgun sequence.
ACCESSION   DAZGQL010000047 DAZGQL010000000
VERSION     DAZGQL010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36526
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.047"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            332..781
                     /gene="glmS"
                     /locus_tag="ACHKIR_002590"
     CDS             332..781
                     /gene="glmS"
                     /locus_tag="ACHKIR_002590"
                     /EC_number="5.4.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050097 - methylaspartate mutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase subunit S"
                     /protein_id="HFO9990312.1"
                     /translation="MKKATLVIGVIGADCHAVGNKVLDRVFSNHDFRVINLGVMVSQD
                     EYIDAAIETGADAIVVSSIYGHGDIDCLGMRERCIERGLGDILLYVGGNLVVGKHDFA
                     DVETKFKEMGFDRVFAPSHDLEDVCQLMAHDINQRHDVDTRILEEAI"
     gene            781..2169
                     /gene="glmL"
                     /locus_tag="ACHKIR_002591"
     CDS             781..2169
                     /gene="glmL"
                     /locus_tag="ACHKIR_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase accessory protein GlmL"
                     /protein_id="HFO9990313.1"
                     /translation="MQIVSVDIGSTWTKAALFAREGDALTLVNHVLTPTTTHHLAKGF
                     FSSLDQVLNVDDALPLLNSGEVALKYSSSAKGGLAVAAMGLVPSITLETAKVTAHSAG
                     AKIAQSYAYKLNRRDIQALEETQPDILLFTGGTDGGEESYGLNNARVLAESKLDCAII
                     YAGNRDIQDEVQEILGHKDLTIVDNVLPDLDHPNPLAARQAICDIFLKRIVKGKGLDV
                     VVDKTGEEPMPTPWTVFELVKAISNVDHSWKEFMLIDMGGATTDVYSASANTLSPDTV
                     LHGVPEPFVKRTVEGDLGMRVSAMVVGESAKELVNVNFAQQPAQLDAFYHYLRHLVAH
                     PDYLPANAEEKYFDTVLAGLCVGYATERHAGTKKEVCTCVGNVDLQMGRDLTTVRKVV
                     GSGGWLSRASQFDMHHWLKYRELNDDGKRILLPTEFEYYRDSRGLLPLLANVARVDPL
                     AAARTSIQCLTL"
     gene            2188..2673
                     /locus_tag="ACHKIR_002592"
                     /pseudo
     CDS             2188..2673
                     /locus_tag="ACHKIR_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308789.1"
                     /note="methylaspartate mutase subunit E; catalyzes the
                     formation of threo-beta-methyl-L-aspartate from
                     L-glutamate; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase subunit E"
     gene            3063..4460
                     /locus_tag="ACHKIR_002593"
     CDS             3063..4460
                     /locus_tag="ACHKIR_002593"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001577995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HFO9990314.1"
                     /translation="MHHLSRLDKAFLRKGIQLSRANNIVSLSRPSGASARVLLPDELP
                     LEEKAVTQLLDFAEVKLPDHPGYVRQTCATPDFHPGNGVPVGAVVASTPDLVIPAAIG
                     TDISCGMRLLTTGLTLEQAEAHKDVIAQRLTQVLLQNERDVPLSAQAFTALFDGGPAD
                     CVSTLPSEGLWIRTNIPRMVQEIDRCIGLNEHNSHSRYAPPALQATDRALLRDPCLGT
                     PGSGNHFIELQVVDSVVDRHVAWQQGLKTGDIVVMIHSGSRDVGFYVGRHWMDRARAE
                     WPTGHKHPQSGLYGLAGGLAEEYLQAIGMAARYAWLNRVTIAEMVRVCLANLFHQDNS
                     HLVVDVPHNIILREHEMNIHRKGATPARVGNLALIPGSMGDYSWLAVGCGNPEWLWSC
                     SHGAGRSQRRQAMRSRATEESTLPWQCVTLREERRFEEAPAAYKDIGPVIEAQQEAGL
                     IQPAVRFRPWLTFKG"
     gene            complement(4605..7238)
                     /gene="mngB"
                     /locus_tag="ACHKIR_002594"
     CDS             complement(4605..7238)
                     /gene="mngB"
                     /locus_tag="ACHKIR_002594"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000648955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HFO9990315.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKQVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLTFGEPMKIGYLPDSFGMSGQLPHIYNGFGITRTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVLPLGYAIGKYLPADEDGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMDKLREIYPQRKFVMSRFEEVFEKIEAQRDNLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKIAHARIENKIVNLLEPLATLAWTLGFEYHHGLLEKLWKEILK
                     NHAHDSIGCCCSDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGTLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVITSANAKPQCEITHEAWQSRAVIR
                     YDMAVPRNLHERSARQSTGRVGVELAVTLSDNSRRIDVDINLDNQADDHRLRVLVPTP
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQEGWKEAPVPVWNMLNYAALQEGRNGLAVF
                     SEGLREYEVIGEEKKTFAITLLRGVGLLGKEDLLLRPGRPSGIKMPVPDSQLRGSFSC
                     RLSLFSYTGTPSTAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVVLRLFNPAESTTCEATVAFSREVISCTQTMMDESISTAERD
                     NLKELGAFLPGQSRTFSYRLG"
     gene            complement(7256..9172)
                     /gene="mngA"
                     /locus_tag="ACHKIR_002595"
     CDS             complement(7256..9172)
                     /gene="mngA"
                     /locus_tag="ACHKIR_002595"
                     /EC_number="2.7.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HFO9990316.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LKEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPPNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTAEQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQALNLKRSDETRTVQQDT
                     QPVKSLKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLMRWVKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            9342..10064
                     /gene="mngR"
                     /locus_tag="ACHKIR_002596"
     CDS             9342..10064
                     /gene="mngR"
                     /locus_tag="ACHKIR_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HFO9990317.1"
                     /translation="MGRKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLTDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITAQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            complement(10168..11037)
                     /gene="sucD"
                     /locus_tag="ACHKIR_002597"
     CDS             complement(10168..11037)
                     /gene="sucD"
                     /locus_tag="ACHKIR_002597"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HFO9990318.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(11037..12203)
                     /gene="sucC"
                     /locus_tag="ACHKIR_002598"
     CDS             complement(11037..12203)
                     /gene="sucC"
                     /locus_tag="ACHKIR_002598"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HFO9990319.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(12297..13514)
                     /gene="odhB"
                     /locus_tag="ACHKIR_002599"
     CDS             complement(12297..13514)
                     /gene="odhB"
                     /locus_tag="ACHKIR_002599"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HFO9990320.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPVKESAP
                     AAPAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            complement(13529..16330)
                     /gene="sucA"
                     /locus_tag="ACHKIR_002600"
     CDS             complement(13529..16330)
                     /gene="sucA"
                     /locus_tag="ACHKIR_002600"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HFO9990321.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(16631..17347)
                     /gene="sdhB"
                     /locus_tag="ACHKIR_002601"
     CDS             complement(16631..17347)
                     /gene="sdhB"
                     /locus_tag="ACHKIR_002601"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HFO9990322.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(17363..19129)
                     /gene="sdhA"
                     /locus_tag="ACHKIR_002602"
     CDS             complement(17363..19129)
                     /gene="sdhA"
                     /locus_tag="ACHKIR_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HFO9990323.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASASDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(19129..19476)
                     /gene="sdhD"
                     /locus_tag="ACHKIR_002603"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(19129..19476)
                     /gene="sdhD"
                     /locus_tag="ACHKIR_002603"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HFO9990324.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(19470..19874)
                     /gene="sdhC"
                     /locus_tag="ACHKIR_002604"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(19470..19874)
                     /gene="sdhC"
                     /locus_tag="ACHKIR_002604"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFO9990325.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            20255..20362
                     /locus_tag="ACHKIR_002605"
     CDS             20255..20362
                     /locus_tag="ACHKIR_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9990326.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            20568..21851
                     /gene="gltA"
                     /locus_tag="ACHKIR_002606"
     CDS             20568..21851
                     /gene="gltA"
                     /locus_tag="ACHKIR_002606"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HFO9990327.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            22241..22807
                     /locus_tag="ACHKIR_002607"
     CDS             22241..22807
                     /locus_tag="ACHKIR_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO9990328.1"
                     /translation="MFKGQKTLAALAISLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLRLVNCDLENSNNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            22858..25317
                     /gene="ybgQ"
                     /locus_tag="ACHKIR_002608"
     CDS             22858..25317
                     /gene="ybgQ"
                     /locus_tag="ACHKIR_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO9990329.1"
                     /translation="MDTVNIYRLSFISCLVMAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKGKGVIVPGEYFVSVAVNNNQISNGQKIDWKKNGDQTIPCINDLLVDKFGLKPEVRQ
                     SLPRLNQCVDFSSRPEILFIFDQASQQLNITIPQAWLAWHSDNWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSNSTNLNAYGTAGINAGAWRLRSDYQLNQTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSSNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTVSHSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRANNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRTSMSHQTENETFFSNEVSWGMLSNTSLYGGLLISGDD
                     YHSAAMGIGQNMLWLGALSFDVTWASSQFDTQQDERGLSYRFNYSKQVDATDSTISLA
                     AYRFSDRHFHSYANYLDHKYNNSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGNWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSTGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDNSGISVGMVGEEGHAWLSGVAENQQFTVIWGDSQR
                     CSLHLPEHMEDTANRLILPCH"
     gene            25332..26063
                     /gene="ybgP"
                     /locus_tag="ACHKIR_002609"
     CDS             25332..26063
                     /gene="ybgP"
                     /locus_tag="ACHKIR_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO9990330.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPSPEKNSGHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVITNTGSYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCRLQAVEAKK"
     gene            26060..27121
                     /gene="ybgO"
                     /locus_tag="ACHKIR_002610"
     CDS             26060..27121
                     /gene="ybgO"
                     /locus_tag="ACHKIR_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO9990331.1"
                     /translation="MSAGKGVLLVICLVFLPLKSALALNCYFGTSGGTVEKSEAIQPF
                     AVPGNAKPGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVNPYPGVQDRYYQLGVTY
                     NGVDYDASLGKSRIDTNQCIDSKNINIYTPEQIIAMGWQNKICSGDPANIHMSRTFLA
                     RMRLYVKIREIPPHDYQSSLSDYIVVQFDGAGSVNEDPTAQNLKYHITGLENIRVLDC
                     SVNFSISPETQVIDFGKFNLLDIRRHTMSKTFSIKTTKSQNDQCTDGFKVSSSFYTEE
                     TLIEEDKALLIGNGLKLRLLDENSSPYTFNKYSEYADFTSDMLIYEKTYTAELSSIPG
                     TPIEAGPFDTVVLFKINYN"
     gene            27274..28320
                     /gene="abrB"
                     /locus_tag="ACHKIR_002611"
     CDS             27274..28320
                     /gene="abrB"
                     /locus_tag="ACHKIR_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFO9990332.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAVNWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTVGCLLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATNPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(28317..29108)
                     /gene="nei"
                     /locus_tag="ACHKIR_002612"
     CDS             complement(28317..29108)
                     /gene="nei"
                     /locus_tag="ACHKIR_002612"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000703 - oxidized pyrimidine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HFO9990333.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKPYQSQLIGQH
                     VTHVETRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEEPQTMRVLRVKLQTVDKTIL
                     LYSASDIEMLTPEQLITHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLDIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(29144..29878)
                     /gene="pxpA"
                     /locus_tag="ACHKIR_002613"
     CDS             complement(29144..29878)
                     /gene="pxpA"
                     /locus_tag="ACHKIR_002613"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HFO9990334.1"
                     /translation="MKIDLNADLGEGYASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSVMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALILVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVTAQTVCLH
                     GDGEHALAFARRLRSTFAEKGIVVAA"
     gene            complement(29868..30800)
                     /gene="pxpC"
                     /locus_tag="ACHKIR_002614"
     CDS             complement(29868..30800)
                     /gene="pxpC"
                     /locus_tag="ACHKIR_002614"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HFO9990335.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLKDGDRLPIGKSKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            complement(30794..31450)
                     /gene="pxpB"
                     /locus_tag="ACHKIR_002615"
     CDS             complement(30794..31450)
                     /gene="pxpB"
                     /locus_tag="ACHKIR_002615"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HFO9990336.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAAGPDL
                     AVVAAHCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGIYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(31473..32216)
                     /gene="ybgI"
                     /locus_tag="ACHKIR_002616"
     CDS             complement(31473..32216)
                     /gene="ybgI"
                     /locus_tag="ACHKIR_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HFO9990337.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            32487..33968
                     /gene="dtpD"
                     /locus_tag="ACHKIR_002617"
     CDS             32487..33968
                     /gene="dtpD"
                     /locus_tag="ACHKIR_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HFO9990338.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYSD
                     NHAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFSHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDIFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(34011..35429)
                     /gene="phrB"
                     /locus_tag="ACHKIR_002618"
     CDS             complement(34011..35429)
                     /gene="phrB"
                     /locus_tag="ACHKIR_002618"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HFO9990339.1"
                     /translation="MTTHLVWFRQDLRQHDNLALAAACRNSSASVLALYIATPRQWAA
                     HNMSPRQAEFINAQLNGLQIALAEKGIPLLFHEVDDFAASVEIVKQVCAENRVTHLFY
                     NYQYEVNERARDVQAERALRNVVCEGFDDGVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIDPAPSITLNYPRQPFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSIWLNEL
                     IWREFYRHLITYYPSLCKHRPFIVWTDRVQWQSNSAHLKAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEKFDREGEFIRQWLPELRDVPGKVVHEPWKWAQKAGVTLDYPQP
                     IVDHKEARLRTLAAYEEARKGA"
     gene            complement(35426..35935)
                     /gene="ybgA"
                     /locus_tag="ACHKIR_002619"
     CDS             complement(35426..35935)
                     /gene="ybgA"
                     /locus_tag="ACHKIR_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HFO9990340.1"
                     /translation="MNQQRFDESTLIRIFALYELHRLKEHGLTRGALNDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDYYNQYRQRVVVLLSHPANRRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRGGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            complement(36077..36526)
                     /locus_tag="ACHKIR_002620"
                     /pseudo
     CDS             complement(36077..36526)
                     /locus_tag="ACHKIR_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT         8854 a         9757 c         8977 g         8938 t
ORIGIN      
        1 taacttttat gaaatgcatt tactcatggc taaccccata tcactaaaat gcaaattaga
       61 agttgatcac aattatctca atacatcaaa tcaagttaat tttaaaaatt taacattcat
      121 gacattagtt gacataacac actgaattaa catgagaatt tgaaaacaca ctttaaacaa
      181 tcatttcccg aaagacattt tgaaaataga tcaattgcaa cattacggtc agataaagac
      241 actcctgtga tattaaacct gtgccgttat gagcggccat aaatacgacg tgtgtaactt
      301 attttactct ctgtcttaca aaggattacc catgaagaaa gcaacacttg tgattggcgt
      361 tattggcgcg gactgccatg cagtaggcaa taaagttctg gaccgcgttt ttagcaacca
      421 tgacttcaga gttattaatc tgggcgtaat ggtcagccag gacgaatata ttgatgctgc
      481 cattgaaact ggtgctgacg cgattgtcgt ctcctctatc tatgggcatg gcgatatcga
      541 ctgtctgggc atgcgcgaac gctgcatcga gcgtggactg ggggacatcc tgctctatgt
      601 gggcggtaac ctggtggtcg gaaaacatga cttcgctgac gtggaaacca aattcaaaga
      661 gatgggattt gatcgcgtct ttgccccaag tcatgactta gaagatgttt gtcagctgat
      721 ggcccacgat atcaaccaac gtcatgacgt tgatactcgt attcttgaag aggcaatctg
      781 atgcaaatcg tctctgtcga tattggatcg acgtggacca aagcagccct cttcgcccgg
      841 gagggggacg cgttaacgtt ggttaaccac gtactgaccc caaccaccac ccatcatctg
      901 gcgaaggggt ttttctccag cctggaccag gtgttaaacg tagatgatgc cctccccctg
      961 ttaaacagtg gtgaggttgc attgaagtat tcctcctctg ccaaaggtgg gctggcggtt
     1021 gccgcaatgg gattggtgcc ttctatcacc ctggaaaccg cgaaagtaac cgctcactct
     1081 gcgggtgcta aaattgcgca atcttacgcc tacaagctga accgccgtga tatccaggca
     1141 ctggaagaaa cgcagcccga tattctcttg ttcaccggcg gcaccgatgg cggagaagaa
     1201 agctacggac tgaataacgc ccgtgtgttg gcagaatcca aactcgactg cgccattatt
     1261 tatgccggaa accgggatat tcaggacgag gtgcaggaga tcctcgggca taaagacctg
     1321 accatcgtcg acaacgttct gcccgatctt gaccatccga accctttagc tgcacgccag
     1381 gctatctgcg acatattcct taaacgcatc gttaaaggga aagggctgga tgtcgttgtt
     1441 gataaaacag gcgaagaacc aatgccgaca ccgtggacag tctttgaact ggttaaggca
     1501 atcagtaatg tcgatcacag ctggaaagag ttcatgctga tcgacatggg tggcgcaacc
     1561 accgatgtct attccgccag tgccaacacg ctctcgcccg acactgtttt acatggcgta
     1621 ccggaaccct tcgtaaaacg gacggtggaa ggagatctgg gaatgcgcgt ctcggcaatg
     1681 gtggttggtg aaagtgcgaa agagttagtc aatgtgaatt ttgctcagca acccgcacag
     1741 ctggacgctt tttatcatta cctccgtcat ctagtcgctc atcccgacta cttacccgcg
     1801 aatgcagaag aaaaatattt cgataccgtc ctggccggac tgtgtgtggg ttatgccacc
     1861 gaaagacatg cgggcaccaa aaaagaggtc tgcacctgcg tcggcaatgt cgatttacag
     1921 atgggacgtg acctgacaac cgtgcgtaaa gtcgtcggat ccggcggatg gctctcgcgt
     1981 gccagtcagt tcgatatgca tcactggttg aaataccgcg agttaaacga cgacggtaaa
     2041 cgcattctct taccgaccga atttgaatat taccgcgatt cccgggggct attaccactg
     2101 ctggcaaacg tcgccagagt cgatccgctg gccgccgcgc gcaccagtat tcaatgctta
     2161 accctataac aattaaggct gcaatcaatg gaacttcgaa ataagaaatt aacccatgac
     2221 gaattcatga ccgagcggca acaggtactg aaaacctggg aaaccggcaa ggatgttgaa
     2281 aactttgaag atggcgttaa ataccaacaa accattcctg agcataaacg cttctctctt
     2341 gcgctgttga aagcagacaa ggaaggtaaa acccttagcc aaccgcgcgc aggtgtggcg
     2401 ctgatggacg aacacatcga actactgaaa acactacaag aagagtgcga tctgctgccg
     2461 agcaccatcg atgcatatac ccgtctgaat cggtacgaag aagcggctgt agggattaaa
     2521 aaatccatcg aagccggaac ctctaaactg aacggtttac cggtcgttaa ccacggcgtt
     2581 gcagcttgcc gtcgcctcac cgaaactctg caaaaaccgc tacagatccg ccacggcacg
     2641 ccagatgcgc gtctgctggc tgaaatctcg atgtgtatac atgaatatac tctaaataag
     2701 tcaagttgca ggaagggaac aaataatgaa tttccagaag cttacataag taagtgacct
     2761 gggtgagcga acatcgatgc agcacatgca acttgaatta tgacaagtat acaatgataa
     2821 atttagatac cctatgttat atattatcta tgtccggtta aattgaatga gtcatggcct
     2881 taaaagactt cacaaaagat aattcctctt taacaataag ccagatttac cacttgtaac
     2941 acttgctatc gccacataca ctacgcgcgt tttctttccc ttttgctggc tgctgaccgg
     3001 aaagacaaaa ttaccctcgc catacagggg gttgtttaac gtctatattt caggacagcg
     3061 ttatgcatca tctttcaagg cttgataaag cctttctgcg caaagggatc caactttccc
     3121 gcgccaacaa tattgtttca ttatctcgtc catcaggtgc cagtgcccgc gtactgctgc
     3181 cggacgaact gccgctggaa gaaaaagcag taacacaact gcttgatttt gcagaagtga
     3241 agctaccaga ccacccaggt tatgtgcgcc agacgtgcgc gacgccagat tttcatcccg
     3301 gcaatggcgt ccccgttggc gcggtggtgg cctcaacgcc ggatttagtc attcccgccg
     3361 ccatcggcac cgatatttct tgtggcatgc ggctgctgac gaccggatta acacttgagc
     3421 aagccgaagc gcataaagac gtcatcgctc agcggttaac gcaagtgcta ctgcaaaatg
     3481 agcgcgatgt tccgttatcc gcacaggcgt tcaccgcgct ttttgatggc ggaccggcag
     3541 attgcgtgtc gaccttgcca tcagaaggtc tctggatacg cacgaatatc ccccgaatgg
     3601 tacaagagat tgaccgctgc attgggctta acgagcataa cagtcattcg cgttatgccc
     3661 cacccgccct gcaggcaacg gaccgcgcat tactccgcga tccttgtctg ggtacgccgg
     3721 gttcaggtaa tcattttatt gaacttcagg tcgttgatag cgtggttgat cgccatgtag
     3781 cctggcaaca agggctgaaa acaggcgata ttgtggtgat gatccacagt ggatcgcgag
     3841 atgttggctt ttacgtcgga agacactgga tggatcgcgc ccgtgctgaa tggcccaccg
     3901 gacataaaca tccacaaagc gggttatatg gtctggcagg cggactggcg gaggaatatc
     3961 tacaagcgat aggaatggcg gcgcgttacg catggttaaa tcgcgtcaca attgccgaaa
     4021 tggtgcgcgt atgtcttgcc aatctctttc atcaggacaa ttcgcatctg gtggtggatg
     4081 tgccacataa catcatcctt cgcgagcatg aaatgaatat tcatcgcaaa ggcgcaacac
     4141 cggcacgagt gggcaatctg gcgttaatcc ccggttcaat gggcgattat tcgtggcttg
     4201 cggtaggttg cggcaacccg gagtggctgt ggtcatgcag tcacggagca ggacgcagcc
     4261 agcgtcggca ggccatgcgc tcacgcgcga ctgaagagtc aacgctgccg tggcaatgcg
     4321 tcacattgcg cgaagaacga cgttttgaag aagcgccagc ggcttataaa gatattggtc
     4381 cggttatcga agcccaacaa gaagccggat taattcagcc agcggtcaga tttcgaccgt
     4441 ggttaacgtt taaggggtaa ctgaaatgat aaatggcctg cggttctcac gcaggtcaaa
     4501 tctggtatct gcgtaagcta acaaacttgt ttatcaacaa tctcaggcca tccgaagatg
     4561 gcctgaaatt attgcccccc tcagatacac ttttagcgca ataatcagcc aaggcggtag
     4621 ctaaatgtcc gtgactgccc cggtaaaaat gcccccagtt cctttaaatt atctctttcc
     4681 gctgtgctga tgctttcatc catcatcgtt tgcgtacatg aaatcacctc acgactgaac
     4741 gcgacagttg cctcacaggt tgtcgattct gccggattaa acagtcgcaa taccacttct
     4801 tgccggtctt ccgctttttt aagagcgcta atcaggcatc ccacaggggg cattttcagc
     4861 aagctatagc tttccggcac attgaatccg gctttattta acttcatcgc atcccacggg
     4921 attttgttgt aacactgtac tggcgtcagc catgcgcgcg cctgctgggc tacaccggcg
     4981 gtcgaaggcg tacctgtata actaaataag ctcagacggc agctaaagga gccacgtaat
     5041 tgtgaatcgg gcaccggcat ttttattcct gaaggacgtc caggccttaa aagcagatct
     5101 tctttcccca gtaagcccac gccacgcagc aacgtaatgg caaaggtttt cttctcttca
     5161 ccgatgactt catattcacg tagcccttcg ctaaagacag ccagaccgtt acgcccttcc
     5221 tgtaaggcag catagttgag catattccat accggaaccg gcgcttcttt ccagccttct
     5281 tgctgccagt tgttcattgc actgtcgttc acggggcgcg ttagcgaacc aaactgggta
     5341 tcggccagaa cactgtcggt gttaaaaggt gtagggacca ggacgcgaag gcgatgatcg
     5401 tcagcctggt tatcaaggtt gatatccaca tcaatgcgcc tgctgttatc actcagagtg
     5461 acagccaact ccacccctac tctgccagtg gattgtcttg cgctgcgttc atgcagatta
     5521 cgcggcaccg ccatgtcata gcggataaca gccctactct gccaggcctc atgagtaatc
     5581 tcgcattgcg gtttcgcgtt cgccgaagta attacccact cttcttttgc gggtgaatag
     5641 tcatattcat caccatcatc agagctttct tcaatttgca acacccggtc atagcgcaca
     5701 ccgctgtctt tatctaccag ttgtagcgtt ccatcctcat tgagcgcaat ttgccagaaa
     5761 gcattttcca gtatcccttc agcgtcactt tttgccgcaa tcacgttgcc aggctgattc
     5821 gcttcgatat aaagcgtgcg atagcccata gaagggacaa tctggttgat ctgaatatca
     5881 aactccataa agggatcata attaccgtaa tgaacgattt gccgatcgat taggccagga
     5941 tcgatctcac gggcatggcg aataaaatac ggtacaggct gaccgcgatc gtcccgcaaa
     6001 ttaaactggc tggcgcgcag ccgcacagtg gtgttgatca cttcttcccg cggccagggc
     6061 atcaggttaa acagcacaag tttgtcggca tcactctgtg gcatgttatc ggcaatttta
     6121 cgcatataga aacgcagcag attatccgcc atgtcttctg ccagttcgaa acgggcgaca
     6181 atctcgcgat gaactttgtc actacagcag cagccgatgc tgtcatgggc gtggtttttt
     6241 aaaatttctt tccacaattt ctccagcaag ccgtggtggt attcaaaacc caacgtccag
     6301 gccagcgttg ccagcggttc cagcagatta acaatcttat tttcaatacg cgcgtgggca
     6361 attttgatat ccatacgcgt agaaccgatg gtgcgatgca cgcgcatata tttgccatca
     6421 ataaattccc ctttcagggt tgccagatta tctcgctgcg cttcgatctt ctcaaatacc
     6481 tcttcaaagc ggctcatcac aaacttacgt tgagggtaga tctcacgtag cttatccatc
     6541 acttcgaaga tattttgctg caatggcatc tggtcatgac cattcggcag caaaatctct
     6601 ttggttactg acgctttttc cagcacatca aaataactgt cgaggcgttt gcgtaatccg
     6661 tcttcgtcgg caggtaagta cttaccgatg gcgtagccca gcggcagcac ctgcgccgtc
     6721 acttcgctac cgtcactgct ttgccacaaa aactcggttt tatcagtacc gtggcgctca
     6781 gaacatccgc gccagaacat ggtgcgggta atgccaaatc cattgtaaat atgcggcagt
     6841 tgcccggaca tgccaaagga atctggtaaa taacctattt tcatcggctc gccaaacgtg
     6901 aggcagtcac gcattccgta catcagatta cggacgatgg attccgcaga aacaatcgtg
     6961 gtatcggtct gggtatacca ggggccgata atcaacttgc ctgcttctac ctgtttcttc
     7021 acacggtctt tgttttccgg tttcaccgcg aaataatctt cgaggatcgc cgtttgcccg
     7081 tcgagtacgt aatatttgta ttcgttgtcc tgttccagtc ggcacaggat ctcttccata
     7141 ttattgacca gcagaatgcg tgactcttcg gtggtgaaat accactcccg atcccagtgc
     7201 atatgcgggg tgatgtgaac gcgagatacc gctttcatcg tcgtttcctt tttgtttatg
     7261 gcattacgcc atcagtcaga tagttgccat gcttaaccgc gtgacgccgc cagatcagaa
     7321 gaattgcagt cgagattgca gcccccacca atgccgcacc aaaccagcca attgccgcca
     7381 taacaccgcc cgcgccatta tcatgaagta aaaagagcga gaaaatgccg gcaccgggtg
     7441 tcgaaaggcc gatattcatc gcgccgacga tagcgcccgt taccatagag cccagcacaa
     7501 acgaaccaat aacccgcagc ggatcttcaa tcgccatcgg gatcgcccct tcggtaatac
     7561 ctgccagccc taacagccag gtggatttcc cggtctcaat ttcaaactct ttaaacaggc
     7621 gcggtgcgag catcgtggaa gcggttacgg taaatgccga aaccattttg acggaggcga
     7681 aaatggcata cgggccgtaa acgccgttcg ccattgcgcc caggcagaat gcataagcgg
     7741 ctttgttcac tggccctcca aggtcaaagg aacacataaa accgagaatg gcacccagca
     7801 acagcgcgtt acttcctgac agaccgttca gccaggcggt aagcgagtta ttgatccagg
     7861 cgacaggttc ccccaccaca aacagcatca gactgcccgc tcccaacgta ccgagcaccg
     7921 ggtagagata aaaagtcagg aatccattga atttactgct aagacgcaag tgatttttca
     7981 cccagcgcat cagataaccg gctatcaatc cgccaacgac agcaccgaga aacccggaac
     8041 cgatcatgtt ggcggcaagt ccagccgcaa agcctggcgc taacgccggt ttatcagcca
     8101 gtgaataggc ggtgtaggct gccagcaccg gtaccatcaa aatcccgagc agccctccgc
     8161 ccagtttgcg gtacatccac agccaggagt tttcttcatt aaacaaatct tgtagcccga
     8221 agatttgcga cagtaatacc gcaaccgcca gcaccgtgcc ccccgcgaca atcaacggca
     8281 ctgcaaaaga gatcccgctc aacagtgcct gttttagctc cgttttgagg cttttcaccg
     8341 gttgcgtatc ttgctgtacg gtacgcgtct catcgctacg cttgaggtta agcgcttgtt
     8401 ggatcaacgc ttctgcatgg cgaatcggct cggcaacagg cacagaaagc gcgggaatgc
     8461 cgttaaaacg ctcactctcc ttgatggcga cttcagccgc aaaaatacag gcggttgcac
     8521 tattgagttg ctctgccgtt aaacgccctt caatgccgtt agcaccttgc ttttcaacgt
     8581 agacattcac gccgagtttg cgtccggctt tttccagata ctccgcagcc atataggtgt
     8641 gagcaatacc cgccgggcag gccgttacgc agacgatagt tggcgcgttg gaaaaagaag
     8701 ctgagggttg cgtgcctccc ttgtcatcca gcgccgagag caattcatca ggcgtcgtcg
     8761 ccgactgtat acgcgcccgt atctcatcat ccgccaggcg cgtggtcagc gctgtcagca
     8821 gttgcatgtg cgtagtaccc gcttcattgg ggggaatcgc cagcaacacc actaaatcaa
     8881 ccgcttccgg gccatccacg ccttcccact gaagcggctc gctgagtgtg gcgaccgcaa
     8941 acgccgcttc ttttaccgca gcagttttgc catgcggcac agccagccct tcacctaagg
     9001 cggtcgggcc aaggctttca cggcgataca cttctttcag aaattgctca gtactggaaa
     9061 ttttccccag agcagcaaga cgttgagtca acgcgtggat ggcctcttca cggctggtaa
     9121 agcgcgcatt cagacacaac gcatcgcggt gggtcagagt cgtcaggttc atcatgatcc
     9181 tcacttgatc gtttttatag tcgcgccagt gtgcccgata aaacaatacc attatgtgat
     9241 caatttcact tgattgaaat tatttgtatt gtatttgtat tatttacagg atgaatttat
     9301 cgggcataat ggctaaattc gtattaatga gatgctaaat catgggacgg aagcccttat
     9361 accgacagat tgctgatcgc attcgtgagc aaattgcgcg tggcgagctt aaacctggcg
     9421 atgcgttacc cactgaatca gcgctacaaa cagagtttgg cgtcagccgg gttacggtgc
     9481 gtcaggcttt gcgccagtta gttgaacagc agatcctcga aagtattcag ggcagcggga
     9541 cttacgtaaa agaagagcgg gtcaattacg atatttttca gttaaccagt tttgatgaaa
     9601 aactgacgga ccgtcacgtc gatacacaca gtgaagttct gatattcgaa gtgattccgg
     9661 ctgacgattt tcttcagcaa cagctacaaa tcaccgcgca ggatcgtgtg tggcatgtga
     9721 agcgcgtgcg ctatcgcaag caaaagccaa tggcgctgga agaaacctgg atgccgcttg
     9781 cgttgttccc ggatctcacc tggcaggtca tggaaaattc gaaatatcac tttattgaag
     9841 aagtgaagaa aatggttatc gatcgtagcg aacaggaaat cattcctctg atgccaaccg
     9901 aagaaatgag ccgtctgctt aatatcagcc agactaagcc gattcttgaa aaagtatcgc
     9961 gtggatattt agttgatggc cgggtgtttg aatatagccg caacgctttt aataccgatg
    10021 actataaatt taccctgata gcgcagcgaa aatcatcgcg ataagcataa aaaaggccat
    10081 catacgatgg ccaaccatgt cgaaaccgac agcccttcgc cattcctgta gaaggaagcg
    10141 gcgagggcta tttcttatta cagatattta tttcagaaca gttttcagtg cttcaccgat
    10201 atccgccagg ctgcgaacgg ttttcacgcc tgcggcttcc agagcagcga atttctcatc
    10261 cgcagtccct ttcccaccgg caatgatcgc acccgcgtgg cccatacgtt tgcctttcgg
    10321 cgcagtcaca ccagcgatgt aacctacaac tggcttggta acgtgctctt tgatgtacgc
    10381 cgctgcttct tcttcagcgc taccgccgat ctcaccgatc atcacgatcg cttcggtctg
    10441 cggatctttt tcgaacattt cgaggatgtc gataaagtta gagccaggga tcggatcgcc
    10501 gccgatacca acacaggtcg actgaccgaa accgtaatcc gtggtctgtt taaccgcttc
    10561 ataggtcagc gtaccggaac gggaaacgat acccacttta cccggtttgt gaatgtgacc
    10621 aggctggata ccgattttgc attcacccgg agtgataacg cctgggcagt tcgggccgat
    10681 catacgaacg cctgcttcat ccagcttcac tttcacggtc agcatatcca gcgtcgggat
    10741 gccttcagtg atggtgataa tcagtttgat gcctgcatcg atagcttcca gaatggagtc
    10801 tttgcagaac ggtgctggta cgtagataac agaagcggta gcgccagtag cagcaacagc
    10861 ttcacgcacg gtgttgaaca ccggcaggcc gaggtgggtg gtgccgcctt tacctggggt
    10921 tacgccgcca accattttag tgccgtatgc aatggcttgt tcagagtgga aagtcccctg
    10981 gctaccggta aagccctggc agataacctt ggtgttttta tcaattaaaa tggacattat
    11041 ttcccctcca ctgcggcaac aacctgctga gctgcatccg tcagaccttt tgctgcaata
    11101 atattcaggc cgctgtcagc cagtttcttc gcgccgagtt cggcgttgtt accttccaga
    11161 cgtaccacga ccggtacgtt aacacccact tccgctaccg cgccgatgat accgtcagcg
    11221 atcaggtcgc aacgaacgat accgccgaag atgttaacca gaacggcttt cactttgtcg
    11281 tcagagagga tgattttgaa cgcttcggtt acacgttctt tggttgcgcc gccgccaacg
    11341 tccaggaagt tagctggttc gccgccgtgc agtttaacga tgtccatggt gcccatcgcc
    11401 aggcctgcgc cgttaaccat acaaccgatg ttaccgtcca gcgcaacgta gttcagttcc
    11461 cactgtgcag cctgtgcttc acgcggatct tcctgcgact ggtcacgcat ttcgcgcaga
    11521 tcaggctggc ggaacagtgc gttgccatca gcgcccagtt tgccgtcgag gcaaatcaga
    11581 tcgccctgtt tggtaatgac cagcgggttg atttcgatca gcgccaggtc gcgctccagg
    11641 aaaatggtcg ccaggcccat gaagattttg gtgaactgct gaaccagttt accttccaga
    11701 cccagtttga acgccagctc gcgtccctga tacggcatcg ggccagtcag cggatcaagc
    11761 gcaactttgt ggatcaggtg cggagtttct tccgccactt tttcgatttc cacgccgcct
    11821 tcggtggagg ccataaagac cacacgacgg gaactacggt caacaacggc accgagatac
    11881 agctctttag cgatatcggt cgctgcttca accagaatct ggttaaccgg ttggccattg
    11941 gcatctgttt gatacgttac cagacgcttg cccagccagt tttctgcaaa agcacggatg
    12001 tcttctttgc tgtttacaac tttcacaccg cccgctttac cgcggccacc agcgtgaacc
    12061 tgacatttca ctacccacgg accggcaccg atttttgaag cggcttcttc tgcttcgcgc
    12121 ggagtagtac aggcataacc caccggtgct ggtaagccat agcgggcaaa aagttgtttt
    12181 gcctgatatt catgtaagtt catgtgttct gtccatcctt cagtaatcgt tatcttttaa
    12241 accgtagacc gacaccctat ccacgtcggt ctacagtgca ggtgaagctt aaactactac
    12301 acgtccagca gcagacgcgt cggatcttcc agcaactctt tgatcgttac caggaagccc
    12361 acggattcgc gaccatcgat cagacggtga tcgtaggaca gcgccaggta catcatcggc
    12421 aggatctcaa cctgaccatt caccgccatc ggacgatctt tgatagcgtg catacccaga
    12481 attgcgctct gcggtgggtt gatgataggc gttgacatca gggaaccgaa cacaccaccg
    12541 ttggtgatgg tgaagttacc accagtcaga tcttcaaccg tcagcttgcc gtcacggcct
    12601 ttaacagcca gctctttgat tttcttctcg atgtctgcca tgccgagggt atcgacatca
    12661 cgcagaaccg gcgtcaccag gccgcgcggc gtagaaaccg ccatactgac gtcgaaatag
    12721 ttgtggtaga ccacatcatc accgtcgata gacgcattca cttccgggta acgtttcagg
    12781 gcttcaacca ccgctttcac gtagaaggac ataaagccca gacggatgcc gtggcgtttt
    12841 tcaaacgctt caccgtactg cttacgcaga tccataatcg gcttcatgtt gacttcgttg
    12901 aacgtggtca gcatggcggt ggagtttttc gcttccagca gacgctctgc cacacgctta
    12961 cgcaggcgag tcatcggcac acgtttttcg ctacgtgcag ccagggccgg ttgcgccgcc
    13021 ggagcaggcg ctgccggagc agactctttc accggggctt tcgccagatg tttttccaca
    13081 tcttcacggg tcagacgacc acccacaccg gtgcctttaa tggcgctggc gtcgaggtta
    13141 tgttcagcca gcaggcgacg gatcgccggg cttaacgcat cgttgttttg ctcttccaga
    13201 gacgcctgct ggcgttgcgc cggagtggac gctttctctt cagatttggc gctggtttct
    13261 ttacccgcgc tgttgccttc acgcaggcga ccaaggatct gacgagacgt taccgttgta
    13321 ccttcatctt ccagaaccgc atccagaatg ccgtctgctg atgccggtac ttccagtacc
    13381 actttgtcag tttcgatttc taccagcact tcatcacgta cgactgcgtc gccgggtttt
    13441 ttatgccagg ttgcgacggt ggcatcggct acggattcag gcaggtcagg gaccagaata
    13501 tctacgctac tcattgtgta tcctttattt attcgacgtt cagcgcgtca ttaaccagat
    13561 cttgttgctg tttctggtga acggacatat accctaccgc cggagaggcg gaggccgggc
    13621 ggcctgcata acgcagagaa gccccaaacg gaatcacttc acggaaatga tgctggctgc
    13681 agtaccatgc gccctggttg agcggctctt cctggcacca gacaaaatcc ttgacgtgag
    13741 caaactgctg caacacttcc tgcatcgctt tatgcgggaa cgggtagagt tgctcgatac
    13801 gcacaatggc gacatcgtgt tgattgttct tacgacgttg ttccagcagg tcgtaataaa
    13861 ccttaccaga acacatcact acgcgcttca cgcccttcgg atcaagctcg tcgatttcac
    13921 cgatggctgg caggaaggtg ccgttcgcca gttcttcgag gctggatacc gccagcggat
    13981 gacgcagcag ggatttcggc gacatcacga ccagtggacg acgcatcccg cgcaacgcct
    14041 gacgacgcag catgtggtaa acctgtgccg gggtagacgg tacgcaaacc tgcatgtttt
    14101 gctcagcgca aagttgcaga taacgttcca gacgcgcgga ggagtgctcc ggcccctgcc
    14161 cttcgtaacc gtgcggcagc aacatcacca ggccacacat ccggccccat ttctgttcgc
    14221 cagaggagat gaactggtcg ataaccacct gcgcaccgtt ggcgaagtca ccgaactgcg
    14281 cttcccagat ggtcagagtg cgtggttctg cggtggcata accgtattca aacgccagca
    14341 cggcttcttc tgacagtacg gagtcccaga cacggaacgc gccctgaccg ttatggatgt
    14401 gttgcagcgg cgtgtaagtg gaaccgttag actggttgtg gatcaccgcg tggcggtgga
    14461 agaaggtacc gcgaccggag tcttcacccg acaggcgaac cggaatgcct tcatcaacca
    14521 gcgtggcgta agcgaggttt tccgcaccgc cccagtcgaa cagtttctca ccggctgcca
    14581 ttgcctggcg atcgccataa atcttggcaa cgcgagactg catttcaact gcttccggca
    14641 ccgtgctgat gcgtttagcc agttcctgca ggcgcttcat ctcaactttg ttcgggtact
    14701 cttcgtccca ttcgtggttg aggtacggcg accaggtgaa agagtgcatg ttcatcggac
    14761 gccactctgc tacaacgcaa tcgccagcat ccagcgcatc gcggtacagg ttaaccatct
    14821 cggtggcatc ttccagcgtc gcgacttttt cctgctccag cttgtcagca tagattttgc
    14881 gcggcgtcgg atgttttttg attttctgat acatcagcgg ctgggttgcg ctcggctcgt
    14941 cggcttcgtt gtggccgtga cggcggtagc acaccagatc gatgaagaca tcacgtttaa
    15001 aggtgttacg gaaatcgagc gccagacggg tcacaaaggc aacggcttcc ggatcatccg
    15061 cattaacgtg gaaaatcggt gcctgtacca tcttgccgat atcagtacag tacggcgtcg
    15121 aacgggcatc cagcgggtta gaggtggtga agccaacctg gttgttgata acgatacgta
    15181 ccgtaccgcc aacttcataa ccacgcgctt tcgacatgtt cagggtttcc tgaaccacgc
    15241 cctgcccggt cactgcggcg tcaccatgaa tggtgattgg cagcactttg ttgctgctcg
    15301 gctcatcaag tctgtccaga cgggcacgaa cagaaccgat aacgaccggg ctgacaatct
    15361 caaggtgaga cgggttaaac gccagcgcca ggtgcaccag gccgccatcg gtctggaagt
    15421 cagacgagaa gcccatgtgg tatttcacgt cgcccgtgcc gaggtgttct ttatgtttac
    15481 cggcaaactc gtcgaacaag tcttgcggtt ttttacccag cacgttcacc agcacgttca
    15541 gacgaccacg gtgcgccatc ccgagaacca cttcgcgggt gccgctgttg ccagcgtggc
    15601 ggatcatctc tttaagcatc gggattaacg cgtcaccacc ttccagcgag aagcgttttg
    15661 cgccagggaa ttttgcgccg aggtaacgtt caaggccttc agcggcggtc agttcgctta
    15721 agaagcgttt tttctcttcg ctattgaaag tcgcgcgacc agactcgata cgctgttgga
    15781 tccagcgttt ttcttcagtg ctggtaatgt gcatatactc ggcaccaatc gggccgcagt
    15841 aggtttgctt gagggcttcc agcagctcgc cgagtttcat ggtttctttg ccgctggcaa
    15901 atgaaccgac gttgaaggtc tcctggaagt ctgcttcggt cagatcgtgg aaagacggat
    15961 ccagatcggc cactttatct tgctgccaca gtcccagcgg atcgagattc gcatgctggt
    16021 gaccacggaa gcggtatgcg ttaatgagct gcaggacttt aacctgcttc acattggtgt
    16081 cagggtcgga gatcgttgaa gagtaacgtg aagcgtcttt cgccaggcgg cggaaatatt
    16141 cacgcgtttg agagtggaat tgatccggtt tgactcccgt accaggtaac tgctggaacg
    16201 tcgaacgcca gttagcgtca accgagtcag gatcggttaa gaagtcttca tagagctgtt
    16261 ctatccagct ctggtttgcg ccagagaggt aagaagagtc caaccaggct ttcaaagcgc
    16321 tgttctgcat cgtgatccct taagcatctt ttttatgctt acttcgccgt ggatactacc
    16381 acgcacagtg atgtgcgtgc cggggttcac ttgcgagctc ttgtctacgt agtggctcgc
    16441 gaaggaacct ttaaaaactg tcttagtgtc aggggcagtt tttaaagatt tcctgcattt
    16501 ctttgttacg cctgatgcgc tgcgcttatc aggcctacgg gtaatgactt gtaggccgga
    16561 taaggcgttc acgccgcatc cggcactggt tgcctgatgc gacgcttgcg cgtcttctca
    16621 ggcctacggt ttacgcatta cgttgcaaca acatcgactt gatatggccg atggcgcgcg
    16681 tcgggttcag ccccttcgga catacactga cgcagttcat gatgctgtga cagcggaata
    16741 cactgaatgc atcgctcaaa ccgtcgaggc ggctgtcagt ctcggtatca cggctatcga
    16801 tcaggaaacg atatgccgct aacaagcctg ccgggccgat aaacttatcg ggattccacc
    16861 agaaagacgg acaagaggtt gaacaacatg cgcagagaat acattcatac aacccgtcga
    16921 gtttttcgcg ctgctctggc atctgtaaat gctcgcgagc tggcggattt tgtccattat
    16981 tcaacaggta aggcttaatt ttctcatatt gcgcatagaa ttgtcccatg tctaccacca
    17041 aatcgcggat caccggtaaa cctggcagcg ggcgaatcac aatcttcttg cccggctggt
    17101 tgagtgccga aatcggggta atacaggcca gaccattctt gccgttcatg ttcagaccgt
    17161 cggaaccaca cacaccttca cggcaggagc gacggaacga caggctggga tctttctctt
    17221 tcagctggat aagcgcatcc agcagcatca tgtcacgacc ttcttccgct tccagggtgt
    17281 aatcctgcat acgcggagca tcatcaacat ccgggttata gcgataaatt gaaaactcga
    17341 gtctcatttt cctgtctccg cattagtaag tacgaatctt cggcgggaat gccgggcgca
    17401 gtttcggttc catgttgacg cttcggcgcg tcatggattc cgactcaggc agatacaggg
    17461 agtggcacag ccagttttca tcatcacgat ccgggaagtc gaagcggcta tgtgcgccac
    17521 ggctttcggt acggaagttg gcagaaacag ccgttgcata cgccgtttcc atcaggttat
    17581 ccagttccag gcactcaacg cgctgggtat tgaactcact tgaagtgtca tccagacggg
    17641 catttttcag acgctcgcgg ataactttca actgctcaag ccctttcgcc atcgcatcac
    17701 cttcacggaa gaccgagaag ttatgctgca tacattcttg cagtgcttta cggatcgcca
    17761 ccggatcttc accgttacgg ttattgttcc agcggttcag gcgatccaga gacgcttcta
    17821 catcagacgc gctggcatcg cgcagtgcgc cctgctcggc gatagactct tgcagatgca
    17881 gacctgccgc acgaccaaat acgaccaggt ccagcagcga gttgccgccc agacggttag
    17941 cgccatgtac cgatacacaa gcgatttcac caacggcaaa tagccccgga acaaccacat
    18001 cttcgccttt ctcgttcacc gtcagcgcct gaccggtcac tttggtcgga ataccgccca
    18061 tcatgtagtg acaggttggg ataaccggaa tcggctcttt caccggatca acgtgagcga
    18121 aggtgcggga gagttcaagg atacccggca gacgggattc aagaacttct ttacccagat
    18181 ggtcaagttt cagtttcgcg tgtggccccc acggaccatc acagccgcga ccttcacgga
    18241 tttcaatcat gatggaacgc gccaccacgt cacggcccgc caggtctttg gcgttcggtg
    18301 cataacgctc cataaagcgt tcgccatgtt tgttcagcag ataaccgcct tcaccacggc
    18361 acccttcggt gaccagtacg cccgcaccgg caataccagt cgggtggaac tgccacattt
    18421 ccatatcctg cactggtacg cctgcacgga tagccatgcc gacgccgtcg ccagtgttaa
    18481 tgtgggcgtt ggtagtggac tgataaatac gccctgcccc gccagtcgcc agcacggtag
    18541 cgcgagcttt gaagtaaacc acttcaccag tttcgatgca cagtgcggta caaccgacca
    18601 ccgcgccatc ctggtttttc accagatcca gcgcatacca ctcggagaaa atggtggtgt
    18661 ggtttttcag gttctgttga taaagggtgt gcaacagtgc gtgaccggta cggtcagctg
    18721 ccgccgcagt acgtgccgcc tgctcgccgc cgaagttttt cgactgaccg ccaaacggac
    18781 gttgatagat acgaccatca tcaagacgcg agaacggcag gcccatatgt tccagttcca
    18841 gaatcgcttc cggcccggtt ttacacatat attcaatcgc gtcctggtca ccgatatagt
    18901 ccgaaccttt tacggtgtcg tacatatgcc attcccagtt atcttcatgg gtattaccca
    18961 gcgcaacggt aatgccgcct tgcgcagaaa cggtatggga acgggtcggg aagactttag
    19021 agagcagcgc acaggtctga ccgctctggg aaatttgcag cgccgcgcgc atacctgcgc
    19081 caccggcacc aattacaact gcatcaaatt ctctgactgg caatttcatc acacacccca
    19141 caccacaacg aatccataaa tcacgtaaac caccagagca acgacaatca ccagttgcag
    19201 catcaggcgc aaggccagcg gtttaacgta gtcggtcaac acctgccaca tgccgatcca
    19261 ggcatggatc aagatagaaa acagcgccag cagggtgaac actttggtga acgcagaggc
    19321 gaagaaaccg atccagactt catatgtcag ctcgccactg gtagcgaaaa aaccgaccat
    19381 ataaatgatg tagagcgtca ggacgatagc ggtagcgcga acgaggatga aatcatgtac
    19441 gccattgcgt cctaatgcgg aggcgttgct taccatacga ggactcctgc gagaagtgaa
    19501 agcacgacag taataacaaa ggatattttg gcggagcgtt tacccgcttc gaatgtttct
    19561 tccagatagc caaaatccat catcatgtgg cgaataccta cgacgacgtg atacgccaga
    19621 gcggtaagga tgccccacat gataaatttg acgaagaagc tgcccataat cgcggaagct
    19681 tgctcgaaac cttcagggga agagaggctg gtacccagaa gccacagcag gatgcccact
    19741 gcaacaaagg tgatcacacc ggaaacgcga tggagaatgg acgctatcgc cgtgatgggg
    19801 aaccggatgg tctgtaggtc cagattaaca ggtctttgtt ttttcacatt tcttatcatg
    19861 aataacgccc acatgctgtt cttattattc cctggggact acgggcacag aggttaactt
    19921 tctgttacct ggagacgtcg ggatttcctt cctccggtct gcttgcgggt cagacagcgt
    19981 cctttctata actgcgcgtc atgcaaaaca ctgcttccag atgcgaaaac gacacgttac
    20041 aacgctgggt ggctcgggat tgcagggtgt tccggagacc tggcggcagt ataggccgtt
    20101 cacaaaatca ttacaattaa cctacatata gtttgtcggg ttttatcctg aacagtgatc
    20161 caggtcacga taacaacatt tatttaattt ttaatcatct aatttgacaa tcattcaaca
    20221 aagttgttac aaacattacc aggaaaagca tataatgcgt aaaagttatg aagtcggtat
    20281 ttcacctaag attaacttat gtaacagtgt ggaagtattg accaattcat tcgggacagt
    20341 tattagtggt agacaagttt aataattcgg attgctaagt acttgattcg ccatttattc
    20401 gtcatcaatg gatcctttac ctgcaagcgc ccagagctct gtacccaggt tttcccctct
    20461 ttcacagagc ggcgagccaa ataacaaacg ggtaaagcca ggttgatgtg cgaaggcaaa
    20521 tttaagttcc ggcagtctta cgcaataagg cgctaaggag accttaaatg gctgatacaa
    20581 aagcaaaact caccctcaac ggggatacag ctgttgaact ggatgtgctg aaaggcacgc
    20641 tgggtcaaga tgttattgat atccgtactc tcggttcaaa aggtgtattc acctttgacc
    20701 caggcttcac ttcaaccgca tcctgcgaat ctaaaattac ttttattgat ggtgatgaag
    20761 gtattttgct gcaccgcggt ttcccgatcg atcagctggc gaccgattct aactacctgg
    20821 aagtttgtta catcctgctg aatggtgaaa aaccgactca ggaacagtat gacgaattta
    20881 aaactacggt gacccgtcat accatgatcc acgagcagat tacccgtctg ttccatgctt
    20941 tccgtcgcga ctcgcatcca atggcagtca tgtgtggtat taccggcgcg ctggcggcgt
    21001 tctatcacga ctcgctggat gttaacaatc ctcgtcaccg tgaaattgcc gcgttccgcc
    21061 tgctgtcgaa aatgccgacc atggccgcga tgtgttacaa gtattccatt ggtcagccat
    21121 ttgtttaccc gcgcaacgat ctctcctacg ccggtaactt cctgaatatg atgttctcta
    21181 cgccgtgcga accgtatgaa gttaatccga ttctggaacg tgctatggac cgtattctga
    21241 tcctgcacgc tgaccatgaa cagaacgcct ccacctccac cgtgcgtacc gctggctctt
    21301 cgggggcgaa cccgtttgcc tgtatcgcag caggtattgc ttcactgtgg ggacctgcgc
    21361 acggtggtgc taacgaagcg gcgctgaaaa tgctggaaga aatcagctcc gttaaacaca
    21421 ttccggaatt tgttcgtcgt gcgaaagata aaaatgattc tttccgcctg atgggcttcg
    21481 gtcaccgcgt gtacaaaaat tacgacccgc gcgccaccgt aatgcgtgaa acctgccatg
    21541 aagtgctgaa agagctgggt accaaagatg acctgctgga agtggctatg gagctggaaa
    21601 acatcgcgct gaacgacccg tactttatcg agaagaaact ctatccgaac gtcgatttct
    21661 actctggtat catcctgaaa gcgatgggta ttccgtcttc tatgttcacc gtgatcttcg
    21721 ctatggcacg caccgttggc tggatcgccc actggagcga aatgcacagt gacggtatga
    21781 agattgcccg tccgcgtcaa ctgtatacag gatatgaaaa acgcgacttt aaaagcgaca
    21841 tcaagcgtta atggttgatt gctaagccgt ttactttccg gacccgcctt taatagagcg
    21901 ggttcttttt tatatggctg aacataataa attgtaaaaa ttaaaccaga tgcaaatacc
    21961 cattaataag gtgaaaaaat gtgacaagtc acatactgag cagaatgata agtttatttt
    22021 tttactttcc tgccggtagc attgttaacc caatgaaaat tatgcatatc catcccaccc
    22081 catctttcat ctcattaaac aataaattta tgcactcaat tattccattc tttatataac
    22141 aagcttttta atattgttaa ataaaaccaa atacattcaa ctgtcagtta atatattctt
    22201 gcctcgcaac actctatctc attatttaaa ggactcagct atgtttaaag gacaaaaaac
    22261 attggccgca ctggccatat ctctgctatt cacagcacct gtttatgcgg ctgatgaagg
    22321 atccggtgaa attcacttta aaggtgaagt tattgaagca ccgtgtgaaa tacatcagga
    22381 tgatattgat aaagaggttg aactcggtca ggtgaccacc agccacatta atcagtcgca
    22441 tcacagcgat gccgttgctg tcgacctgcg cttagtcaac tgcgatctgg aaaactccaa
    22501 caacggttcc ggcggcaaga tttctaaagt tgcagtcacc tttgacagct cagcgaaaac
    22561 caccggcgca gatccgattc tcaacaacac cagcacgggt gaagcgaccg gtgtcggcgt
    22621 gcgtttaatg aataaagatc aaagcaatat cgttttaggc accgctactc cagatatcga
    22681 cctggcacca acctccagcg aacaaacgtt aaatttcttt gcgtggatgg aacaaattga
    22741 tcaggctacg cccgtaacac caggcgcagt tacggcgaat gcgacctacg tactggatta
    22801 taaataagta tcactttggc ggcgtcatcg tcgcctttct tcaaatattt atcaggtatg
    22861 gacaccgtga atatttatcg actctctttt atatcctgtc tggtcatggc gatgccttgc
    22921 gcattggcgg tcgaattcaa cctgaatgtc ctcgataaat cgatgcgaga tcgcattgat
    22981 atttcattat taaagggaaa aggggtcatt gttcccggtg aatattttgt tagcgttgca
    23041 gtaaataaca accaaatcag taacggacaa aaaattgact ggaaaaagaa cggtgaccaa
    23101 accattccgt gtattaatga tttactggta gataaatttg gtttaaaacc tgaagttcgt
    23161 cagtcgttac cacggttaaa ccagtgcgtc gattttagct cccggcctga aattctcttc
    23221 atttttgatc aggctagcca gcaactgaat atcactattc cacaagcctg gctggcgtgg
    23281 cactcagata actggactcc cccctcaaca tggaaagaag gtgtcgccgg cgtcctgatg
    23341 gactacaacc tgtttgcaag cagctaccgc ccacaggatg gtagcaacag cactaacctg
    23401 aacgcctatg gaacagccgg aattaacgcg ggagcatggc gtttacgcag tgattaccaa
    23461 ttgaatcaga ccgatagcga tgataaccat gaacagtcag gcgaaatatc acgcacctat
    23521 ctttttcgtc cattaccgca attaggttca aaattaaccc ttggtgaaac ggatttcagt
    23581 tccaatattt tcgacggctt ttcttatacc ggcgcggcac tggcaagtga cgatcggatg
    23641 ttaccgtggg agctacgcgg ctacgcccca caaatcagcg gcattgcaca aaccaacgcc
    23701 accgtcaccg tgagtcactc agggcgagtt atttaccaga agaaagtccc accaggtccg
    23761 tttatcattg atgaccttaa tcagtccgtc cagggtacgt tagatgtcaa agtgacggaa
    23821 gaagatggtc gggcgaacaa tttccaggtt tcggcagcat cgacgccttt ccttacgcgc
    23881 caggggcagg ttcgctataa acttgctgcc ggtcagccgc gaacatctat gtctcatcaa
    23941 actgaaaatg aaaccttttt tagcaatgaa gtttcctggg ggatgctgtc aaatacctca
    24001 ctgtacggcg gcctgctgat ttcgggtgac gactaccatt ctgccgcaat gggtatcggg
    24061 caaaatatgc tgtggcttgg tgcattgtcg tttgatgtca cgtgggccag tagtcagttt
    24121 gatactcagc aggacgagcg gggcttaagt taccgcttta attacagcaa acaagtggat
    24181 gccaccgaca gcacgatttc gctcgccgct tatcgtttct ccgatcgtca ttttcacagc
    24241 tacgccaact atctggatca caaatacaac aacagcgatg cgcaggacga aaaacagacg
    24301 atcagcttat ccgtgggcca accgattacc ccactgaacc tcaatctgta cgccaacctg
    24361 ctgcatcaaa cctggtggaa tgcagacgcc tccacgaccg ccaacatcac ggcgggtttt
    24421 aatgttgata ttggtaactg gagagatatc tcgatctcga cgtcattcaa tacaacccat
    24481 tacgaagata aagatcgcga caatcagatt tacctgtcga tttcgctccc cttcggtaac
    24541 ggggggcggg tcggttatga catgcaaaac agtagtcaca gcaccacgca ccgcatgtcg
    24601 tggaatgata cgttagatga acgtaatagc tggggcatgt ctaccggact gcaatccgac
    24661 cgtccggata atggagccca ggtgagcggt aactatcagc acctgagttc agcgggtgag
    24721 tgggatattt ctggtaccta tgccgccaat gattacagtt ccgtcagcag cagctggagc
    24781 ggttctttca cggcaactca atatggtgcg gcgttccatc gtcgcagctc caccaatgaa
    24841 ccacgtctga tggtcagtac cgatggcgtg gcagatattc cggttcaggg caatctcgac
    24901 tacaccaacc attttggcat tgcggtggtg ccgttgattt ccagttatca gccttccacc
    24961 gtggcggtga acatgaatga cttacccgac ggcgtaacag tggcagaaaa cgttatcaaa
    25021 gaaacgtgga ttgaaggcgc gataggttac aaatcgctgg cttcccgttc cggtaaagac
    25081 gttaacgtca tcattcgcaa cgccagcggt cagttccctc ccctcggtgc ggatatccgc
    25141 caggataaca gcggcattag cgtggggatg gttggcgagg aaggacatgc ctggttaagc
    25201 ggagtcgctg aaaatcaaca atttaccgtg atctggggtg atagccagcg ttgctcgctc
    25261 catcttcctg aacatatgga agacaccgca aatcgcctga ttttaccttg tcattaatta
    25321 aggaaatgaa aatgacattt atgaaaggac tacccctttt gttgttggtt gccagtctgt
    25381 gcagccacgc tgcactacaa cccgatcgca ctcgtattgt gtttaacgct aatgataaag
    25441 ctaccagtct gcgcgtggat aatcgtagcg ataaactccc ctacctcgct tattcctggc
    25501 tcgaaaatga aaaaggggag aaaagcgatg atcttctggt tgccctgcca ccgatacaac
    25561 gtctggaacc gaaagcaaca acgcaggtac ggatagtaaa gcaagcctcg accacaaaat
    25621 taccgggcga tcgcgaaacg ctatttttct acaatatgcg tgaaattcct ccctccccag
    25681 aaaaaaacag cggccatgcc gttctgcaag tcgccattca gagtcgtatc aaggtattct
    25741 ggcgaccggc tgcattacgc aagaaagcag gagaaaaggt cgaactgcag ttacaggtca
    25801 gccagcaggg caaccaactg acactgaaaa atcctaccgc gtattatctg accattgcct
    25861 atctggggag aaatgaaaaa ggcgttcttc ccggcttcaa aaccgtgatg gttgcgcctt
    25921 ttagtacagt aattacaaac accggaagtt atagcggcag tcagttttat ctcggttata
    25981 tggatgatta cggcgcgtta cgcatgacga cgctcaactg cagcggacaa tgccgtttac
    26041 aagcagtgga ggcgaagaaa tgagtgctgg caagggagtg ttgctcgtca tctgtctggt
    26101 atttctgcca ttgaagtctg cgctagcgct gaactgctat tttggtactt ccggcggaac
    26161 ggtagaaaaa tcggaagcga ttcaaccctt tgctgtgcca ggtaatgcca aacccggcga
    26221 taagatctgg gaatctgacg atattaaaat tcccgtctat tgtgacaaca ataccaacgg
    26281 caactttgaa agcgaacatg tctacgcatg ggtaaacccc tacccggggg tacaggatcg
    26341 ttattatcaa ctcggcgtga catacaatgg tgtcgattac gatgccagtc tggggaaaag
    26401 ccgcatcgac accaaccagt gtatcgacag taagaacatt aacatttata cccctgagca
    26461 gattatcgcg atgggctggc agaacaaaat atgctcgggt gaccccgcta atattcacat
    26521 gtcgcgaacc tttcttgctc gtatgcggtt atacgttaag attcgcgaaa taccgccgca
    26581 tgattatcaa agttcgctta gcgactacat cgtcgtgcaa tttgatggtg ccggtagcgt
    26641 taacgaagac cctaccgccc aaaacctgaa ataccatatt actggtctgg aaaacattcg
    26701 tgtactggat tgcagcgtca atttttctat ttcaccagaa acacaggtga ttgattttgg
    26761 taaatttaac ctgctggata ttcgccgcca cacaatgtcg aaaacattca gtattaagac
    26821 gacaaaatca caaaatgacc agtgcaccga cggatttaag gtcagctctt cgttttatac
    26881 cgaagaaaca ttgattgaag aggataaagc actgctgatt ggtaatggac tgaagctacg
    26941 tttgctggat gaaaattctt caccctatac tttcaacaaa tacagcgaat atgctgattt
    27001 caccagcgat atgctgatct acgaaaaaac ctatacggct gaactttcgt ccattcccgg
    27061 cacccccatc gaagcgggtc ccttcgatac agtggtgctt tttaagatta actataacta
    27121 atttatcatg atgccctttg cagttagtaa agggcctctc tttcagaaaa ttgctgattt
    27181 tccccttttc cttgattgct gttatttttt cctggcccgg ctttctttat gctgtatcga
    27241 tacaccaaag aaaaaataat gagggaacga aggatgccag ttttgcagtg gggaatgtta
    27301 tgtgtgctct cacttctcct ttcaattggt ttcctcgcgg ttcatctccc ggcggcgcta
    27361 ttacttgggc cgatgatcgc cgggatcatc tttagtatgc gcggaattac tctgcaactt
    27421 ccccgctccg cttttctcgc cgcccaggcc attcttggct gcatgattgc gcaaaacctg
    27481 accggttcga tcctgaccac gctagcggtg aactggccga ttgtgttagc tattttactg
    27541 gtgacgttac tttccagcgc catcgtgggt tggttattgg tgcgctatag ctcactgcct
    27601 ggaaataccg gtgcctgggg ttcttctcct ggcggcgcgg cggcaatggt cgctatggcg
    27661 caagattacg gcgcagatat tcgcctggtg gcgtttatgc aatatctgcg agtgctgttc
    27721 gtcgcagggg ccgctgttct ggtcacccgc atgatgctgg gcgataacgc tgaagcggtt
    27781 aaccagcaga tcgtctggtt tccgccagtg agcattaatc tcctgcttac gattttgctc
    27841 gcggtcgttg ccggtacagt gggatgcttg ctacgtcttc cttccggcac gatgctcatc
    27901 ccgatgctgg cgggcgcagt gctccagtct ggtcagctca tcaccatcga actaccggaa
    27961 tggctgcttg cgatggcgta tatggcaatt ggttggcgga ttggtcttgg tttcgataaa
    28021 caaatcttac tgcgggcatt gcgcccgcta ccgcaaatcc tgctgtcgat ttttgctctg
    28081 ctggctattt gtgcgggtat ggcgtggggg ctgacccggt ttatgcatat tgattttatg
    28141 accgcctacc tcgccaccaa ccccggcggg cttgataccg tggcggtcat cgccgcaggg
    28201 agcaatgccg atatggcgct catcatggcg atgcaaaccc tgcgcctgtt cagtattttg
    28261 ctgacgggac ctgccattgc gcggtttatt tcaacctatg cgccgaagcg ttctgcctaa
    28321 tgctggcagc cagggcacca gtaaaacggg cgagatgaca gcgtggtttt ctcaatgatg
    28381 ccgccacagc gttcgcacag ttcgccatca cgatgaaaaa ccttaaagcg aaacagcgcc
    28441 ccatgatgct tattctcatc cacctgcccc cgcgtagcgt aggaaaatcg aggaatatcc
    28501 agtaacgcgt gtgcgagtgc atccagttgc gccgcattga gatctttcgc tttatgattt
    28561 ccagtcaacc caacctgcca gaggatctcc acccgcaaat aattgccaag cccagccaga
    28621 aacgcctgat cgagcagtaa tccagcaaac tgacggttac gaaagcgcgg cgacaataac
    28681 cgttccttta ccacctccgg cgtcagattc ggatccagca catcggggcc gactcgttgt
    28741 aaaaacggat gcgtgatcag ttgttccggc gtcaacatct caatatccga ggcgctataa
    28801 agcagaatag ttttgtcaac cgtttgcagt tttacccgca atactcgcat agtctgcggc
    28861 tcttcgccgg tatcaaccac gcgccagacg ccgtaaagct gattatggct gtagagcgtt
    28921 aagtcgttgg aaaaatgagt taacaacgcc ttaccacgcg tttccacatg ggtaacgtgt
    28981 tgaccgataa gttgtgattg ataaggtttt aactgcggga aggcaaacca gacatcagtt
    29041 agtggtttgc ctttgatcgc cgcctccagg ttatccgctg cacggcggat ctccgggcct
    29101 tcaggcatgg tgatatcctt ttttcattct ttattaaacg gggttatgct gcgacaacaa
    29161 tccccttttc ggcaaatgta gagcgtagtc ggcgggcgaa tgccagtgcg tgctcgccgt
    29221 cgccatgcag gcagaccgtt tgcgccgtga ccgttgccca ttcgccggtg atgcttttga
    29281 ctctgccgtg ttgcaccatc tccagcgttt gcgccagcgc ctgttcttcg ttttcaatca
    29341 acgcgcctgg ctggcttcgc ggcaccagcg aaccgtctgc ctgataaccg cgatcggcaa
    29401 acacttcctc gcgcgttgtc aggccatatt gcttgcctgc acgaatcagc tcgcttcctg
    29461 ccagcccgac gagaatcaat gccggatcgc aagcgtatac cgctctggcg atggcgtctg
    29521 ccagttgtgc ctctttcgct gcctggttgt ataacatgcc gtgaggtttg acatgacgca
    29581 taacaccgcc ttgcgcacgg gcaatcgttg ccagcgcgcc aatttgatac agcgtctggg
    29641 cgtaaacggt ttctggcggc agctgcatga cgctgcgccc aaaattttcc ctgtcgggaa
    29701 aactcgggtg agcgccaatc gcaacaccat tttttattgc ttcacgcacg caagcctgca
    29761 tggtttgcgc atcgcctgca tgaaatccac aggcaatatt ggcagaggaa accagcgtta
    29821 atagctctgc gtcgctggca tagccttcgc ccagatcggc gttcaggtca attttcattg
    29881 tgcagccgcc acgctaattg ttcgaaataa cgttgctgat cttgccgtgc tttcagtgct
    29941 tcttccagtg aacactggac aaaatgaatc ggctgaccga gcggaatttg cgccagatgg
    30001 tacatatcag cctcaatgat acaggcaata cgcggataac caccggtggt ctgtgcgtcg
    30061 ttcatcaaca caatcggctg cccgttatgc ggcacctgca ccacgcccgg taacaaacca
    30121 tgagataaca gttcgcgatc ggtggtacgt tttaaaattt gcccctgtaa gcgatagccc
    30181 attcggttac tttgcgagct aagctgccag ggcgaacgcc agaatgcatc ctgcgaggcg
    30241 cgatcgaact catgatattc cggccccggc aaggcgcgaa tgcggttgcc ccacagcagc
    30301 tgtttaacgc cctgcgcttc cataaaatca cgcttcgatt tgccaatcgg gagtcggtca
    30361 ccatccttca gtaaacggcc ttccagcccg ccaatcccca ctttgagatc ggtgctgcat
    30421 gaccccatta ccggcggaac atcaatacca cccgcgaccg ccagataact gcgcatcccg
    30481 tgctgcgggc gtttaagcgt taaacgctga cctgctttca tcggcaagcg ccagccggtc
    30541 cagacggcgt tatcatccag ccgcgcttcg caaccggccc ccgtcagagc aaaccaccca
    30601 tcagtttcga actcaaccgt taactgaccg agcgtgatct ccagcgcggg ggcattggcg
    30661 tcattaccca ccagtaggtt agcaatgcgc agcgcaggca tatccagtgc gccgcagtgg
    30721 ctgatacccg actggcgaaa accgtgacga ccgccatcct gcacagtggt atacataccc
    30781 gcacgaataa tcttcagcat actccctcct tctgcggtac aaagcgcacg ctgtctcccg
    30841 gacgtaataa gatgggttcg tcacgcgccg gatcaaacag gctgagtgag gtatgaccaa
    30901 tcaactgcca gccacccggc gttgccaacg gataaatacc agtctgcggc ccgccgatcc
    30961 cgacagaacc tgccggaacg agcaagcgcg gttcagcgcg ccgtggcgtg tgtagttgtt
    31021 ccggcaaact cccgagatac gggaagcccg gttgaaaacc taaaaaccag accacgtatt
    31081 ccacggagga gtgcaattca acaacctgtt tttcgctcaa tccacaatgc gccgcgacca
    31141 ccgccaaatc cggtccggct gcgccaccgt aaaccaccgg aatttcaata aagcgagact
    31201 ccggctccag cgcctcactc tcctcccacc agcgttgcaa acgctctatg gcatccagcg
    31261 ccagcgactc aggattacgc aaaatcactg tgatattgtt catgccagga atggcttcaa
    31321 ccacattcgg catatccacc agacgctgtg ccagtcgcca gatccgtttc tggctagcca
    31381 gcgtcaccgg cggttccagc tccagcacta ccgccgtttc acctatcaga taacaacgcg
    31441 ctcgttgcac tttcgatccc tctgattatt cgttatgcag gattaggaat atcaataaag
    31501 gtcacatcaa gatcggtatt ttcattcagc cactcgctca atgcgcgaat accaccgcgt
    31561 tcagtggcat ggtgacccgc agcataaaaa tgcaatccct gctcgcgggc tgaatgaatg
    31621 gtctgttctg aaacttcgcc agtaataaaa gcatccacgc caaaacgcgc ggcgctatcg
    31681 ataaaactct gcccgccgcc cgtgcaccag gcgacgcgct gaaccacctc aggtccggta
    31741 tcgccacacc ataacggctt acgtcccaga cgcgcttcaa tccaggaagc cagctccagt
    31801 cccggcacag gcatggtcag ttcgccccac ggcaccaacg gctcaatttc gcccatgacc
    31861 gtgatcccca gtaacgccgc cagttgtgcg ttattaccca gctccggatg cgcgtccagg
    31921 ggcagatgcc agccatacag gttgatatca ttcgccagca acgtttttaa acggttacgc
    31981 ttcattccgc gaatgaccgg agattcacct ttccagaagt agccatgatg cacaatgacc
    32041 gcatccgcgc ccagacgcac cgcttcatcg agcaaagcct ggctggcggt gacaccggta
    32101 acaatttttt gcaccgtctc tttgccttcc acctgcaaac cattcggcgc gtaatcacta
    32161 atcgccgcgc tgttcagttt ttcgttaatc agttgttcca gttcggtgtt tttcatcttc
    32221 gcgccttccg tattctgata gctacaccgt taacatagcg ttacctcgcc tgttcgtcga
    32281 tagccaatct tttgacatat taagaaacaa acccgacgcc aaaagacgca aattgtttat
    32341 atcatcacgc ttcagctaat gtatatttat gcaatcagac agcatattgc ataaataatc
    32401 agatctgtat caaagtttta actttaagga aacacaagca cgccgcgcaa ctattacgtg
    32461 acgttgcagt caattgtgga attattatga ataaacacgc atcacaaccg cgcgctattt
    32521 actatgtcgt tgcgctgcaa atctgggaat attttagctt ttacggcatg cgtgccctgc
    32581 tgatcctcta tctcaccaat caactaaaat atagcgataa tcacgcctac gagttattta
    32641 gcgcctactg ttcgctggta tatgtcacgc caatcctcgg tggctttttg gcggataaag
    32701 ttctcggcaa tcgcatggcg gtgatgctgg gtgcgttgtt gatggcgatc ggtcatgtgg
    32761 tgctgggtgc cagtgagatc catccgtcat tcctctatct gtccctggcg attatcgttt
    32821 gtggctatgg tctgtttaaa tccaacgtca gttgcctgct cggcgagctg tatgagccaa
    32881 ccgatccgcg tcgtgatggc ggtttctcgc tgatgtatgc ggcgggtaac gtggggtcta
    32941 ttatcgcacc tatcgcctgt ggctacgccc aggaagagta cagttgggcg atgggctttg
    33001 gcctggcggc ggttggcatg atcgcgggtc tggtcatttt cttatgtggc aatcgtcatt
    33061 tctctcatac ccgcggcgtt aacaaaaaag tactgcgtgc gacaaacttt ctcctgccca
    33121 actggggatg gctgctggtt ctgctggtgg caacacctgc gctgatcacc gtactgttct
    33181 ggaaagagtg gtcggtatac gccttgattg tcgcgactat tattggcctg ggtgtattgg
    33241 caaaaattta tcgcaaagca gaaaaccaga aacagcgtaa ggagctgggg ctgattgtta
    33301 ccctcacctt cttcagcatg ttgttctggg cattcgcaca acaggggggt agctcgatta
    33361 gcctttatat cgaccgcttc gttaaccgcg atatatttgg ttataccgtt ccgactgcta
    33421 tgttccagtc gattaatgcc ttcgcagtta tgctgtgcgg tgtgttcctg gcgtgggtgg
    33481 ttaaagaaag cgtcgcaggt aatcgtaccg tgcgcatctg ggggaaattt gctcttggtc
    33541 tcggcctgat gagcgccgga ttctgcattc tgaccttaag cgcccgctgg tctgcaatgt
    33601 atggtcactc ttctctgcca ctgatggtgt taggcctggc ggtgatgggg tttgcggaac
    33661 tgtttatcga cccggttgct atgtcgcaga ttacgcgcat tgaaatcccc ggcgtgaccg
    33721 gcgtattaac cggcatttat atgctgcttt ccggcgcgat tgcgaactat ctggccggcg
    33781 tgattgccga tcagacatcg caggcttcgt ttgatgcttc cggagcgatc aactactcca
    33841 tcaatgcata tattgaagta tttgatcaaa ttacctgggg cgcactggcg tgtgtaggac
    33901 tggtgctgat gatttggctg tatcaggcgc ttaagttcag aaaccgcgcg ctggcgctgg
    33961 aatcttaaca ggccagcacc gatgcctgat aagacgctcg ctgcgtttta tcaggctcct
    34021 ttcctcgctt cttcgtacgc tgccagcgtt cgcagccttg cctctttgtg atcaactatc
    34081 ggttgcggat aatccagcgt cacacctgct ttctgcgccc acttccacgg ctcatgcacc
    34141 actttccctg gcacatcgcg cagttccggt agccactggc ggataaactc gccctcacgg
    34201 tcaaatttct cgccctgggt tgtcgggtta aaaatacgaa aatacggcgc tgcatcggtt
    34261 ccggttgaag cggcccactg ccagccaccg ttattggctg ccaaatcacc atcaatcagc
    34321 tgcgacatga aatatcgctc accttcgcgc cagtcgatca ataaatcttt caccaaaaaa
    34381 ctggctgtaa tcatccgtag ccggttatgc atccagccgg tgctgttaag ctgacgcatg
    34441 gcagcatcaa caatcgggta tcccgttttg ccttcctgcc aggcctttaa atgcgcggaa
    34501 ttgctctgcc actgcacgcg gtcggtccag acaataaacg gacgatgttt acacagcgag
    34561 gggtaatacg ttatcagatg acggtaaaac tcgcgccaga tcagctcatt aagccagata
    34621 ctaccggccc caccgtccag cgcctgcggc tgttccgcca acaggcgatg caaacactgg
    34681 cgaggcgata acccgcccgt tgccaggctg gcggacaaac ggctggtgcc ttctaccgca
    34741 gggaaatctc gctgttgctc atactctccg gcaccgttct ggcaaaactg gcgtaattgc
    34801 gcaatcgccg ctttctcttc caccgggaaa tgcgcagtat cgaaaggctg acgaggataa
    34861 ttcagcgtaa tggatggcgc gggatctatc gatccgctac tacgtacttt tggcgcagcg
    34921 acgcactccg gcatcccttc tcgcagccgt ttaagccagg cgtttttaaa tggcgtaaag
    34981 actttgtaca tctcatgatt gccagtcatc accgcccccg gcggcaggat cacgccgtcg
    35041 tcaaatcctt cacacaccac gttacgcagc gccctctcag cctgcacatc ccgcgcccgc
    35101 tcattcacct catactgata gttataaaac agatgggtaa cacgattttc cgcgcacacc
    35161 tgtttaacta tttcgacgct ggcggcaaag tcatccactt catgaaacag caaaggaatg
    35221 cctttttccg ccagcgcgat ttgtagcccg ttcagttgtg cattgataaa ttcagcctga
    35281 cgcggcgaca tattatgcgc cgcccactgg cgtggtgtag cgatatacaa cgccagcacg
    35341 cttgcagacg aattgcggca ggcggcagcc agcgcgagat tatcatgctg acgtaaatcc
    35401 tggcgaaacc agaccagatg ggtagtcata aaactcctga atggcgcaag ttaatcacac
    35461 gcggccaaag ttcgaaataa cgctgccctg aaagccaggc gtcaggataa agcgccatat
    35521 agtgtttgat acgcatcagc ggcgcaagaa gcggttgctc gccaccgcga taactgtcga
    35581 taagcgccgc cagctgctgg cgttctgtgg aatcaatatg agggcgaaaa taaccctgaa
    35641 cgtgcatcaa aacattggtg tggtcacgtc ggttggcggg gtgagaaagc aaaacaacta
    35701 cgcgttggcg gtactggttg taatagtcat ccagattttg ccactggtga atatcagcca
    35761 cgaacgggcc aagtttgcgg tactcgggct gggaatgggc cagaaagacg agtttatagc
    35821 ggctgtgata atcgttgagt gccccgcgcg ttaagccatg ttctttcagt cgatgtaact
    35881 cataaagcgc gaagatacgg attagagtgc tttcatcaaa tcgttgctga ttcattactg
    35941 ctcctgagat attaacagga gtaagtgtag acgttggcaa atgatgtttg tgaagttaat
    36001 cctgttgtag cagggcaaaa ttacgaaagc acgctccccg caaggactga cgccagatat
    36061 tttctgtcca tggctgccct ttccattcaa acgtgaaatc aataagttca tcagggacat
    36121 cgcaaacaat atgaagacgg atttcttctc tgccgtgact cttttcactc attgcgtaac
    36181 tgccatgctc tggattattt aattctttca gcggaaattt ttcctcgaag gctttattta
    36241 gccgcccctg gtttcctttt acagcgaata aataatcacc gccctgtttt tgtatcttct
    36301 ctgcaatatc tttctggcaa cccatcgcat cagttgtgat gatttttcct ttaatattca
    36361 gcatgttaag aagttccggg atagctgtga tctcattgga tttctcatcc gtcttgatct
    36421 gtccgatgac cagactgtgc attgttgaga acgcactaat gacatgaatc gctcccttgc
    36481 ggcgactctt gtcataagag tgccggagcg tttttccatc aattgc
//
LOCUS       DAZGQL010000048        36461 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.048, whole genome shotgun sequence.
ACCESSION   DAZGQL010000048 DAZGQL010000000
VERSION     DAZGQL010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36461
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.048"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(58..1149)
                     /gene="ychF"
                     /locus_tag="ACHKIR_002621"
     CDS             complement(58..1149)
                     /gene="ychF"
                     /locus_tag="ACHKIR_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFO9990341.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEEREEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(1266..1850)
                     /gene="pth"
                     /locus_tag="ACHKIR_002622"
     CDS             complement(1266..1850)
                     /gene="pth"
                     /locus_tag="ACHKIR_002622"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFO9990342.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEIWFTDGLTKATNRLHAFKAQ"
     gene            2128..2406
                     /gene="ychH"
                     /locus_tag="ACHKIR_002623"
     CDS             2128..2406
                     /gene="ychH"
                     /locus_tag="ACHKIR_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HFO9990343.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(2461..4140)
                     /gene="dauA"
                     /locus_tag="ACHKIR_002624"
     CDS             complement(2461..4140)
                     /gene="dauA"
                     /locus_tag="ACHKIR_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HFO9990344.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLMSGVFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(4265..5212)
                     /gene="prs"
                     /locus_tag="ACHKIR_002625"
     CDS             complement(4265..5212)
                     /gene="prs"
                     /locus_tag="ACHKIR_002625"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFO9990345.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(5363..6214)
                     /gene="ispE"
                     /locus_tag="ACHKIR_002626"
     CDS             complement(5363..6214)
                     /gene="ispE"
                     /locus_tag="ACHKIR_002626"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFO9990346.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGAEISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(6214..6837)
                     /gene="lolB"
                     /locus_tag="ACHKIR_002627"
     CDS             complement(6214..6837)
                     /gene="lolB"
                     /locus_tag="ACHKIR_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HFO9990347.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPSNMELTDGGQRIKLKMDNWIVK"
     gene            7051..8307
                     /gene="hemA"
                     /locus_tag="ACHKIR_002628"
     CDS             7051..8307
                     /gene="hemA"
                     /locus_tag="ACHKIR_002628"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HFO9990348.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSDIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDGLGLE"
     gene            8349..9431
                     /gene="prfA"
                     /locus_tag="ACHKIR_002629"
     CDS             8349..9431
                     /gene="prfA"
                     /locus_tag="ACHKIR_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFO9990349.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            9431..10264
                     /gene="prmC"
                     /locus_tag="ACHKIR_002630"
     CDS             9431..10264
                     /gene="prmC"
                     /locus_tag="ACHKIR_002630"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFO9990350.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEITAVDRMPDAVALAQRNAQNLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFIHAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            10261..10653
                     /gene="sirB2"
                     /locus_tag="ACHKIR_002631"
     CDS             10261..10653
                     /gene="sirB2"
                     /locus_tag="ACHKIR_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB2"
                     /protein_id="HFO9990351.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTLLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            10657..11466
                     /gene="sirB1"
                     /locus_tag="ACHKIR_002632"
     CDS             10657..11466
                     /gene="sirB1"
                     /locus_tag="ACHKIR_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB1"
                     /protein_id="HFO9990352.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            11502..12356
                     /gene="kdsA"
                     /locus_tag="ACHKIR_002633"
     CDS             11502..12356
                     /gene="kdsA"
                     /locus_tag="ACHKIR_002633"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HFO9990353.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(12505..12612)
                     /locus_tag="ACHKIR_002634"
     CDS             complement(12505..12612)
                     /locus_tag="ACHKIR_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO9990354.1"
                     /translation="MTLAQFTVIFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            complement(13017..14117)
                     /gene="chaA"
                     /locus_tag="ACHKIR_002635"
     CDS             complement(13017..14117)
                     /gene="chaA"
                     /locus_tag="ACHKIR_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HFO9990355.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSLWHAIWLIVHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            14387..14617
                     /gene="chaB"
                     /locus_tag="ACHKIR_002636"
     CDS             14387..14617
                     /gene="chaB"
                     /locus_tag="ACHKIR_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HFO9990356.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            14775..15470
                     /gene="chaC"
                     /locus_tag="ACHKIR_002637"
     CDS             14775..15470
                     /gene="chaC"
                     /locus_tag="ACHKIR_002637"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HFO9990357.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGLA"
     gene            complement(15514..15867)
                     /gene="ychN"
                     /locus_tag="ACHKIR_002638"
     CDS             complement(15514..15867)
                     /gene="ychN"
                     /locus_tag="ACHKIR_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HFO9990358.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            16053..17447
                     /gene="ychO"
                     /locus_tag="ACHKIR_002639"
                     /gene_synonym="iatA"
     CDS             16053..17447
                     /gene="ychO"
                     /locus_tag="ACHKIR_002639"
                     /gene_synonym="iatA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HFO9990359.1"
                     /translation="MSRFVPRIIPFYLLLLAAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDAFSQQVNQHV
                     ESWLSAWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGSWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSVEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSEEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            complement(17448..18098)
                     /gene="narL"
                     /locus_tag="ACHKIR_002640"
     CDS             complement(17448..18098)
                     /gene="narL"
                     /locus_tag="ACHKIR_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HFO9990360.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(18091..19887)
                     /gene="narX"
                     /locus_tag="ACHKIR_002641"
     CDS             complement(18091..19887)
                     /gene="narX"
                     /locus_tag="ACHKIR_002641"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HFO9990361.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSARFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            20226..21617
                     /gene="narK"
                     /locus_tag="ACHKIR_002642"
     CDS             20226..21617
                     /gene="narK"
                     /locus_tag="ACHKIR_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HFO9990362.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWV
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGRTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            22010..25753
                     /gene="narG"
                     /locus_tag="ACHKIR_002643"
     CDS             22010..25753
                     /gene="narG"
                     /locus_tag="ACHKIR_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HFO9990363.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLLGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLMGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAREAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQALDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVMGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKELGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITSQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            25750..27288
                     /gene="narH"
                     /locus_tag="ACHKIR_002644"
     CDS             25750..27288
                     /gene="narH"
                     /locus_tag="ACHKIR_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFO9990364.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            27285..27995
                     /gene="narJ"
                     /locus_tag="ACHKIR_002645"
     CDS             27285..27995
                     /gene="narJ"
                     /locus_tag="ACHKIR_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFO9990365.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLPKEDAHALG
                     IFLRDLTTMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLAQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVLFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFAEKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            27995..28672
                     /gene="narI"
                     /locus_tag="ACHKIR_002646"
     CDS             27995..28672
                     /gene="narI"
                     /locus_tag="ACHKIR_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFO9990366.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(28857..28988)
                     /locus_tag="ACHKIR_002647"
     ncRNA           complement(28857..28988)
                     /ncRNA_class="other"
                     /locus_tag="ACHKIR_002647"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(29035..29119)
                     /locus_tag="ACHKIR_002648"
     tRNA            complement(29035..29119)
                     /locus_tag="ACHKIR_002648"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(29083..29085),aa:Tyr,seq:gta)
     gene            complement(29279..30121)
                     /gene="purU"
                     /locus_tag="ACHKIR_002649"
     CDS             complement(29279..30121)
                     /gene="purU"
                     /locus_tag="ACHKIR_002649"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HFO9990367.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            complement(30171..30629)
                     /gene="ychJ"
                     /locus_tag="ACHKIR_002650"
     CDS             complement(30171..30629)
                     /gene="ychJ"
                     /locus_tag="ACHKIR_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HFO9990368.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMK
                     DADYLIKTWHPSCGAATLRAELIAGFAHTEWLGLTVFEHCWQDVDNIGFVSFVARFTE
                     GSKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            30742..31647
                     /gene="rssA"
                     /locus_tag="ACHKIR_002651"
     CDS             30742..31647
                     /gene="rssA"
                     /locus_tag="ACHKIR_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HFO9990369.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVEKKMDELLPLVRTNI"
     gene            31739..32752
                     /gene="rssB"
                     /locus_tag="ACHKIR_002652"
     CDS             31739..32752
                     /gene="rssB"
                     /locus_tag="ACHKIR_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HFO9990370.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            32954..33862
                     /gene="galU"
                     /locus_tag="ACHKIR_002653"
     CDS             32954..33862
                     /gene="galU"
                     /locus_tag="ACHKIR_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFO9990371.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(34007..34420)
                     /gene="hns"
                     /locus_tag="ACHKIR_002654"
     CDS             complement(34007..34420)
                     /gene="hns"
                     /locus_tag="ACHKIR_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0071824 - protein-DNA complex organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone-like nucleoid-structuring protein H-NS"
                     /protein_id="HFO9990372.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            35024..35641
                     /gene="tdk"
                     /locus_tag="ACHKIR_002655"
     CDS             35024..35641
                     /gene="tdk"
                     /locus_tag="ACHKIR_002655"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFO9990373.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALEVGSLTAIQERHRHD"
     gene            complement(35924..>36461)
                     /locus_tag="ACHKIR_002656"
     CDS             complement(35924..>36461)
                     /locus_tag="ACHKIR_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS4-like element IS4 family transposase"
                     /protein_id="HFO9990374.1"
                     /translation="WPGLGNEVTARLLTVTRKGKVCHLLTSMTDAMRFPGGEMADLYS
                     HRWEIELGYREIKQTMQLSRLTLRSKKPELVEQELWGVLLAYNLVRYQMIKMAEHLKG
                     YWPNQLSFSESCGMVMRMLMTLQGASPGRIPELMRDLASMGQLVKLPTRRESAFPRVV
                     KERPWKYPTAPKKSQSVA"
BASE COUNT         8958 a         8991 c         9531 g         8981 t
ORIGIN      
        1 cattatgtgc gactcacata atggctcatt attaaccgtc agggtattac gtggaattta
       61 gacgttgaac aggaagttca tcacatcgcc atctttaacg atgtaatctt taccttctgc
      121 acgcattttg cctgcttctt tcgcgccttg ttcacctttg taagtgatga aatcttcaaa
      181 cgagatagtc tgtgcgcgga taaagccttt ttcgaaatcg gtatggattt tgccagccgc
      241 ttgcggtgca gttgcgccga cggggatggt ccatgcgcgc acttctttca ccccagcggt
      301 gaagtaagtt tgcaggttca gcagtttata accggcacgg atcacacggt tcaggcccgg
      361 ctcttccagt cccagttcct gcataaactc ttcacgttct tcatcgtcca gttcggcaat
      421 gtctgcttca acagctgcac aaaccggaac cacaacagaa ccttctttcg ccgcgatttc
      481 acgcacctgg tcaagatacg ggttgttttc aaaaccgtct tcgttgacgt tggcgatgta
      541 catcgtcggt ttcagcgtca ggaagctcag gtaacgaata gccgcttttt cttctgcgct
      601 taaatccagc gcgcgcagca tgcccgcgtt ttccagctgt ggaaggcatt tttccagtac
      661 tgccagttcg gctttcgcgt ctttatcgcc gcctttggct ttcttctgta cgcggtgaat
      721 cgcgcgctcg caggtgtcga gatccgccag tgccagttcg gtattgataa cttcaatatc
      781 gtccgccggg ttaactttac cggaaacgtg aatgatattg tcattctcaa agcagcgaac
      841 aacgtggccg atcgcttcgg tttcacgaat gttggtcagg aactggttac ccagaccttc
      901 acctttcgac gcgcccttta ccagaccggc gatatcgaca aattccatgg tagtgggaag
      961 cgtacgctgc ggttttacga tttcagccag ttgatccagg cgagggtcag gcattggtac
     1021 gacgcccgtg ttcggctcaa tggtacagaa tggaaagttg gccgcttcaa taccggcttt
     1081 ggtcagcgcg ttgaacaggg tagatttccc gacgttgggc aaaccgacga taccgcattt
     1141 gaatcccatg atttaaatca ccttaatatc ttaataatca acctgttatt gctaacagat
     1201 tgcagaaatg gaaataactt tgcctattat acacggcact cggcaaaaat gccgcagaca
     1261 acgacttatt gcgctttaaa ggcgtgcaat cggttcgttg ctttggtcaa gccatctgta
     1321 aaccatattt cagtacaacg tgccgcttcg tcgatggctt catcaattaa cttctgttca
     1381 ctaacaggcg gtttgcctaa cacaaaaccg acaactttat ttttatcgcc tggatgacca
     1441 attccgatgc gtaaacggtg aaagttaggg ttattaccca atttactgat gatgtctttc
     1501 agtccattgt gaccaccatg gccaccgccc aacttaaatt tggcgacgcc aggaggcaga
     1561 tccagttcgt cgtgggccac cagaatctcg tccggattaa tgcggaaaaa actggccatc
     1621 gccgcaacgg ctttgccgct gagattcata aatgtagtcg ggactaacag gcggacatct
     1681 tcgcctccaa gggtgactcg cgaagtataa ccaaagaatt tagcctcttc gcgcagcgga
     1741 gcgcgcaatc gctctgccag taagtcaacg aaccaggcac cagcattatg tcgcgttgcg
     1801 gcgtattcag cgccggggtt cgccaggccg acaatcaatt taatcgtcac gtttttttgt
     1861 cctgagtgtg tacataactg gcgcgtagtt tactgtttgc ggccccgctt gacaaaaaac
     1921 tgcgtatcaa atgcagataa cgcaataatt gcctaagtgg actattagaa agtcaaggtg
     1981 ttcaggcgtt tatttgtaaa gttttgttga aataagggtt gtaattgtga tcacgcccgc
     2041 acataaccca cagggtgttg tctatacttt acacataagg aagaggggta ttccctgtta
     2101 caacccagaa agttccggag gtgacatatg aaacgcaaaa acgcttcgtt actcggtaac
     2161 gtgctcatgg ggttgggtct ggtggtaatg gtggtcggcg tggggtattc aatcctcaac
     2221 cagttaccac agtttaatat gccccagtat ttcgcacatg gtgcagtgct aagtattttc
     2281 gtcggtgcca ttctctggct ggcgggtgcc cgtgttggcg ggcatgaaca ggtgtgcgac
     2341 cgttactggt gggttcgcca ctatgacaaa cgttgccgcc gtagcgataa tcgccgtcat
     2401 agctaacaaa taatgcggtt tgctgaccag tcagtttgcg ctgactggtc aatttcgtac
     2461 ttataaatcc gccatcgccg cgcgacgatt cgggaagaac gccaggcgtc ccgggatcgg
     2521 ttgaatgcct gcgcgagcca tagtgcgcag tggctggaat tccacgttgc acacgcgcag
     2581 ttcacatcct tcgggtagac gcttcacaaa acgctggaac gcatcaagac caccagcatc
     2641 aagtaccgga acggcatccc acttcagaat cacaatccgt ttgccttcaa gacgtgactc
     2701 caggtccgtg aacaagcctt cagcggcagc aaagaacagc gggccaataa cgcgcagaac
     2761 cagaacatcg tctggaacgt ctacgactac cggtgccagg cgagtcatac gtgcgatacg
     2821 acgcataaac agtagcgatg ccagcacgat ccccacgctg atggcaataa ccatatcaaa
     2881 cagcacggtc agcgacatgc acagcagcat gacgatgatg tcatctttcg gcgcatgacg
     2941 cagcaagtcg accactttgt gcgcttcact catgttccac gccaccatca acagcagggc
     3001 agccatggcg gaaagaggca gccaggagag cagcggtgcc agtaccagca gggcaagaat
     3061 aaccagaata gagtggatca ccgccgagat aggggaagtt gccccggcac ggacgttagc
     3121 ggcagaacgc gcgatggcag ctgtagcggt aataccacca aagaacggag cgatgatatt
     3181 ccccagtccc tgtccaacca gttcactgtt cgctttgtgt ttcgtcccgg tcataccatc
     3241 cagcaccacg gcgcagagca gtgattcaat tgcccctaac atcgccatcg aaaatgctgc
     3301 cggaagcaga gtgcgaatgg agtcccaggt gagggtaaat tcggaattgg gcaggtccca
     3361 cggcagtacc agttgcggca gcagttgcgg aataccgtta ccctgagaac catcggccag
     3421 aacgtagtgg aattgcgaac cgatggtagc aacatgtccg ccgagcaggt taacaatccc
     3481 catcaccgcg caaccagcca gcaaggccgg aaggtgaccg ggtaaacgaa tgcccagacg
     3541 tggccagaaa accagaatgc caagcgtcac aatgccaatg gcagcatcac ccacattaat
     3601 ggtcggcaac gccataaata atgcgccgac tttttgtaga taatgttcag ggacatgggc
     3661 catttgcaga ccgagaaaat ctttaatctg catggtaccg atggtgatcc cgataccgga
     3721 agtaaaacct aaggtaacgg aaaccggaat atattcaatc aagcgtccaa aacgtgccag
     3781 ccccatcagg atcaaaaaga cacctgacat gagtgtcgcg accagcagtc ctgccagtcc
     3841 aaactgttgc gaaacgggat agagaattac cacaaatgcc gcagtcggac cggaaacgct
     3901 aaagcgtgac ccacccgtca gagcaatgac aatccccgca acagctgcgg tatataaacc
     3961 gtactggggt gccacaccac taccaatagc caacgccatt gccagcggga tagcaataat
     4021 cccgacggtt atcccggcaa tcaggtcacg ggtaaaccgt gcggcagtat atttttcttt
     4081 ccagcaagcg tcgatcagag cgcggaaagg catcacatgt gaggaaaata ttttgttcac
     4141 aataatgttt catccgtgag cgcatcatct gtcaactaaa tggcaggtga aggaggcata
     4201 ggtcatacaa atggatatta cagacaaaaa aacccgccgc agcgggtctt tgagccgggt
     4261 tcgattagtg ttcgaacatg gcagagatcg attcttcgtt gctgatacga cgaatcgctt
     4321 cggccagcat acctgacagg gtcagagtac gcacgttcgg cagtgatttg atttcatcgc
     4381 tcagcggaat ggtatcgcag acaacgactt catcaattac agagttacgc aggttgttcg
     4441 ccgcgttgcc agagaagatc gggtgagtcg cgtacgcaaa tacacgttta gcaccacgtt
     4501 ctttcagagc ttcagcagct ttacacagcg taccgccagt atcgatcata tcatcgacca
     4561 gtacgcagtc acgacctgca acgtcaccga tgatatgcat cacctgggaa acgttcgcac
     4621 gcggacgacg tttgtcgatg attgccatat cggtatcgtt cagcagctta gcgatagcgc
     4681 gggcacgcac aacgccgccg atgtccggag aaaccacaat tgggttatcc agattcagct
     4741 gcagcatgtc ttccagcagg atcgggctac caaatacgtt atcaaccgga acgtcgaaga
     4801 aaccctgaat ctgttcagcg tgcagatcca ctgtcagcac acggtcaaca ccgacgctgg
     4861 agaggaagtc tgcaaccact ttcgcagtga ttggtacacg agcggaacgg acgcgacggt
     4921 cctggcgcgc atagccaaag taggggataa cagcggtgat acgacctgcg gaagcacgac
     4981 gcagggcatc aaccataacg actaattcca tcaggttgtc gttagtaggg gcacaagtgg
     5041 actggatgat gaaaatatca ccaccgcgta cattttcgtt aatttgtacg ctgacttcgc
     5101 cgtcgctaaa gcgacctaca gcggcgtcgc cgagtgaagt gtacaggcgg ttggcaatac
     5161 gttgtgctag ttccggggtg gcgttaccag caaaaagctt catatcaggc acgagaagaa
     5221 cctcaggcat gcgtccattg gtggaaagaa tctgccgaaa actgtgcggg ccaggcatga
     5281 ttctttccag gcggtgtatt aaagagcgcg atgcaacgtc tggaacaagg tgacgttgtc
     5341 accgaaactc agcttgcccg gcttaaagca tggctctgtg caatggggaa agattaacgc
     5401 ctttcgccac aaagccattg agccattccg gggcttgctc tagcacctgg cgggcttcag
     5461 actctgtatc aaattcagca aagacacagg cccctgtccc agtcaggcgc gacggggcgt
     5521 attctaacag ccaggaaagc accgcatcaa cctcgcgaaa acgttttctt gcgataacct
     5581 cgcaatcatt gctgaattca cattttagca acgtttctat tgaccttttt ggcgtattgc
     5641 gcgggagttc aggatcttta aaaatcaccg gtgtcggaat acttacacca gggtgcgcca
     5701 ccaagtacca cttctctggc ggatccaccg gcgttagtat ttcaccaacg ccttcggcaa
     5761 acgcggcatg cccccgaaca aagacaggaa catctgcgcc cagcgtcagc cccatttccg
     5821 ccagctcatc catgcttagc ccgcattgcc agagatggtt taatgccacc agcaccgtcg
     5881 cggcattgga tgaaccacca cccagaccgc cgcccatcgg caaacgcttg tcaatactga
     5941 tttctgcacc gcttcccgta ggaagacgcc cgctgtctgc cgctgttttc atcagcaacc
     6001 gtgctgcgcg aacgatcagg ttatcttcat gttccacgcc ttcaacgggt gttaacagac
     6061 gaatatcccc atcgtcacga agctcaatgc tgatggtgtc gccgtaatcc aggaactgaa
     6121 acagcgtttg cagcgtgtgg taaccgtccg cacgctgacc ggtaatgtat aaaaacagat
     6181 taagttttgc cggagagggc cactgtgtcc gcattatttc actatccagt tatccatttt
     6241 taacttgatg cgctgaccac catcggtgag ttccatattg gatggcatcg caggttgagt
     6301 ttttgtgtca taaccaccgt aaacaacttt ccagtttttg ccattctggc tgtaggtgat
     6361 ttcgctaagg cggtactggt cgtccagttt gtagtcagtc gcatcacccg gcaaacctaa
     6421 aatccactgg cgtaagctgt tgagtggaat gggcattccg gtcaatttgc caatcatctc
     6481 ttcggcgtca tcggcggtat aacgctggcc tttattgtcg actaactgca cgttacccgg
     6541 ttgagcattc agctccagtt ccgtgctgcc caatgggtta gtcagcagca gacggtagcg
     6601 atcctggcca gtttgctgcc agaagaaacg ggcgtacact ttttgttggt cagaaatata
     6661 agcgaacgcg ccgcgagttt gatactgatt aagattgcgc acgtcttgct gatgctgacg
     6721 ccattgtggc gaatccgggc ttttgccagg acctttgggc gtggtaacgg aacaggcagt
     6781 gagcacaaga gcagccagcg gtagtaggcg gataagacga aaatcgggca ggggcatagt
     6841 gatgacaagt ccttgagata cgttgcagtt ataaccctta atgctagcgt taccgtccgc
     6901 tatcgtctat gttcaagttg tcttaattgc cagaatctaa cggctttcgg caattactcc
     6961 aaaagggggc gctctctttt attgatctta cgcatcctgt atgatgcaag cagactaacc
     7021 ctatcaacgt tggtattatt tcccgcagac atgacccttt tagcactcgg tatcaaccat
     7081 aaaacggcac ctgtatcgct gcgagaacgt gtatcgtttt cgccggataa gctcgatcag
     7141 gcgcttgaca gcctgcttgc gcagccgatg gtgcagggcg gcgtggtgct gtcgacgtgc
     7201 aatcgcacgg aactttatct tagcgttgaa gagcaggata acctgcaaga ggcgttaatc
     7261 cgctggcttt gcgattatca caatcttaat gaagaagacc tgcgtaaaag cctctactgg
     7321 catcaggata acgacgcggt tagccattta atgcgtgttg ccagcggcct ggattcactg
     7381 gttttggggg agccgcagat cctcggtcag gttaaaaaag cgtttgccga ttcgcaaaaa
     7441 ggccatatga aggccagcga actggaacgc atgtttcaga aatctttctc tgtagcgaaa
     7501 cgtgttcgca ctgaaacaga tatcggtgcc agcgctgttt ctgtcgcttt tgcggcttgt
     7561 acgctggcgc ggcagatctt tgaatcgctc tctacggtga cggtactgct ggtgggtgcg
     7621 ggcgaaacca tcgagctggt ggcgcgtcat ctgcgcgaac acaaagtaca gaagatgatt
     7681 atcgccaacc gcactcgcga acgtgcccaa attctggcag atgaagtcgg cgcggaagtt
     7741 attgccctga gtgatatcga cgaacgtctg cgcgaagccg atatcatcat cagttccacc
     7801 gccagcccgt taccgattat cgggaaaggc atggtggagc gcgcattaaa aagccgtcgc
     7861 aaccaaccaa tgctgttggt ggatattgcc gttccgcgcg atgttgagcc ggaagttggc
     7921 aaactggcga atgcttatct ttatagcgtg gacgatctgc aaagcatcat ttcgcacaac
     7981 ctggcgcagc gtaaagccgc agcggttgag gcggaaacta ttgtcgctca ggaaaccagc
     8041 gaatttatgg cgtggctgcg tgcacaaagc gccagcgaaa ccattcgcga gtatcgcagc
     8101 caggcagagc acgttcgcga tgagttaact gccaaagcgt tagcggccct tgagcagggc
     8161 ggtgacgctc aagccattat gcaggatctg gcatggaaac tgactaaccg cctgatccat
     8221 gcgccaacga aatcacttca acaggccgcc cgtgacgggg ataacgaacg cctgaatatt
     8281 ctgcgcgacg gcctcgggct ggagtagcag tacatcattt tcttttttta cagggtgcat
     8341 ttacgcctat gaagccttct atcgttgcca aactggaagc cctgcatgaa cgccatgaag
     8401 aagttcaggc gttgctgggt gacgcgcaaa ctatcgccga ccaggaacgt tttcgcgcat
     8461 tatcacgcga atatgcgcag ttaagtgatg tttcgcgctg ttttaccgac tggcaacagg
     8521 ttcaggaaga tatcgaaacc gcacagatga tgctcgacga tcctgaaatg cgtgagatgg
     8581 cgcaggatga actgcgcgaa gctaaagaaa aaagcgagca actggaacag caattacagg
     8641 ttctgttact accaaaagat cctgatgacg aacgtaacgc cttcctcgaa gtccgtgccg
     8701 gaaccggcgg cgacgaagcg gcgctcttcg ctggcgatct gttccgtatg tacagccgtt
     8761 acgccgaagc ccgccgctgg cgtgtagaaa tcatgagcgc cagcgagggt gaacatggtg
     8821 gttataaaga gatcatcgcc aaaattagcg gtgatggtgt atatggtcgt ttgaaattcg
     8881 aatctggcgg tcatcgcgtg cagcgtgtgc ctgctacgga atcgcagggt cgaattcata
     8941 cttccgcttg taccgttgcg gtaatgccag aactgcctga cgcagaactg ccggacatca
     9001 acccagcaga tttgcgcatt gatactttcc gctcgtcagg ggcgggtggt cagcacgtta
     9061 acaccaccga ttcggcaatt cgtattactc acttgccgac cgggattgtt gttgaatgtc
     9121 aggacgaacg ttcacaacat aaaaacaaag ctaaagcact ttctgtactc ggtgctcgca
     9181 tccacgctgc agaaatggca aaacggcaac aggccgaagc gtctacccgt cgtaacctgc
     9241 tggggagtgg cgatcgcagc gaccgtaacc gtacttacaa cttcccacag gggcgcgtta
     9301 ccgatcaccg catcaacctg acgctctacc gcctggatga agtgatggaa ggtaagctgg
     9361 atatgctgat tgaaccgatt atccaggaac atcaggccga ccaactggcg gcgttgtccg
     9421 agcaggaata atggaatatc aacactggtt acgtgaagca ataagccaac ttcaggcgag
     9481 cgaaagcccg cggcgtgatg ctgaaatcct gctggagcat gttaccggca gagggcgtac
     9541 ttttattctc gcctttggtg aaacgcagct gactgacgaa caatgtcagc aacttgatgc
     9601 gctactgacg cgtcgtcgcg atggtgaacc cattgctcat ttaaccgggg tgcgagaatt
     9661 ctggtcgttg ccgttatttg tttcgccagc gaccttaatt ccgcgcccgg atacggagtg
     9721 tctggtggag caggcactgg cgcggttgcc tgaacaacct tgccgtattc tcgatctcgg
     9781 gacgggtacc ggggcgattg cgctggcgct ggccagcgag cgtccggact gcgaaattac
     9841 cgctgtagat cgtatgccag atgctgtcgc cctggcgcaa cgtaatgccc agaatctggc
     9901 gatcaaaaac atccatattc tgcaaagcga ctggtttagc gcgctagccg ggcagcagtt
     9961 tgcgatgatt gtcagtaatc cgccgtatat tgacgagcag gacccacatc ttcaacaagg
    10021 cgatgtccgc tttgagccgc tcactgcgct ggttgcggca gacagtggaa tggcagacat
    10081 cgtgcatatc atcgaacagt cgcgtaacgc gctggtatcc ggcggctttc tgcttctgga
    10141 acatggttgg cagcagggcg aagcggtgcg acaggcattt atccacgcgg gatatcatga
    10201 cgtcgaaacc tgtcgggact atggtgataa cgagcgcgta acgctcggcc gctattatca
    10261 atgacaagtt tttctacact gcttagtgtt catcttatta gtatcgcgct ttcagttggg
    10321 ctattaacct tacgtttctg gctacgttat cagaagcatc cacaggcatt tgcgcgctgg
    10381 acgcgcattg tgccgccggt tgtcgatacg ctgttattgt taagcggcat tgcgttgatg
    10441 gctaaagcgc acatcctgcc attttccggg caggcacagt ggctgactga aaagctgttt
    10501 ggagttatca tttatatcgt tttgggtttt attgcactcg attatcgtcg tatgcacagt
    10561 cagcaggcgc gcattattgc cttcccgctg gcgttggtgg tgctgtacat catcattaaa
    10621 ctcgccacca caaaagtacc gttactgggg taagtcatga gatcgttagc tgatttcgaa
    10681 tttaataaag cgccattgtg cgaaggcatg atcctggctt gcgaagcaat ccgccgcgat
    10741 tttccctcgc aagatgttta cgacgaactg gagcgtctcg ttagtctggc gaaggaagaa
    10801 atcagccagc ttctgccttt agaagagcag ctggaaaaac tgatcgcgct gttttacggc
    10861 gactggggat ttaaagcctc acgcggcgtt tatcgtcttt ccgatgcatt atggctggac
    10921 caggtgttaa agaatcgaca gggcagtgcg gtatcattag gtgccgtttt attatgggtc
    10981 gcgaatcgtc ttgatttgcc gctgctgccg gtgattttcc ctacgcagct gatattgcgc
    11041 attgaatgtc cggatggcga aatttggctg attaatcctt ttaacggtga atcgttaagc
    11101 gaacatatgc tggacgtatg gttaaaggga aatatcagcc cgtcggcgga actgttttat
    11161 gaagaccttg atgaagctga taacattgag gtaatccgca aattgctgga tacactcaaa
    11221 gcttcgttga tggaagagaa tcagatggag ctggcgttac gcaccagcga agctttatta
    11281 caattcaacc cggaagatcc ctatgaaatt cgcgatcgcg ggttgattta tgcgcaactg
    11341 gattgcgaac acgttgcgtt gaacgattta agttatttcg ttgaacagtg tccggaagac
    11401 ccgatcagcg aaatgatccg tgcgcaaata aataacatcg cgcataaaca tattgtgctg
    11461 cattaattaa tcgacatttt actcaagatt aaggcgatcc tatgaaacaa aaagtggtta
    11521 gcattggcga catcaacgta gcaaatgacc tgccgttcgt actgtttggc ggtatgaacg
    11581 tgctggaatc tcgcgatctg gcgatgcgca tttgcgagca ctacgtaacc gtgacccaga
    11641 aactgggtat cccttacgtg ttcaaagcct cttttgacaa agccaaccgc tcctccatcc
    11701 actcttatcg tggaccgggc ctggaagaag ggatgaaaat cttccaggag ttgaagcaga
    11761 cttttggtgt gaaaattatc accgacgttc acgaaccaag tcaggcacag cccgttgcgg
    11821 atgtcgtgga tgtgattcag ttgccggcgt ttcttgctcg ccagactgac ctggtggaag
    11881 ccatggcgaa aaccggtgcg gtaattaacg tcaagaaacc gcagtttgtt agcccgggac
    11941 agatgggtaa tatcgttgat aaatttaaag aaggcggcaa cgaaaaagtg attctttgcg
    12001 atcgcggtgc taacttcggc tatgacaacc tggttgtcga tatgctgggc ttcagcatca
    12061 tgaagaaagt gtctggtaat tcgccggtga ttttcgacgt gacccacgca ctgcaatgcc
    12121 gcgatccgtt tggcgcagct tccggcggtc gtcgtgctca ggtggctgag ctggcacgtg
    12181 ccggtatggc ggtaggtctg gcggggctgt ttattgaagc gcatccggat ccggaacatg
    12241 cgaaatgtga tggtccatcc gcgctgccgc tggctaaact ggaaccgttc ctcaagcaga
    12301 tgaaagcgat tgatgacttg gtgaaaggtt tcgaagaact ggataccagc aagtaatctt
    12361 ttttgcctga aaactaaagc cttagcgttc tgcgttaagg cttttttcat gggggccgga
    12421 tacaaaaagg gccgcaggct gttacccctg cggccggttt cgggcgcata ttgccatcac
    12481 ggcagcctga cgcccgtttt cgccttactt ccggttacgc caccagccga caatcgctgc
    12541 ggtaataatt cccgccagga tcggtgctgc caggtcgtgc cagaaaatca cggtaaactg
    12601 cgcgagcgtc atatagccgc cttgttgtaa tgacaacatt ttgcggctat tcttgaattg
    12661 ttctggttca agattagccc ccgttctgtt gtcaggttat acctctcaac gtgcgggggt
    12721 tttctctttc cagcaaccaa tgccaccagg gataaagccc ccgcaacatt gcgcctcacc
    12781 ggataacgcc ggcttggtgt ggatactacg actcaattca tcttcacttc atccctgaaa
    12841 tgtttgcaat gaagagtgca ttccggtttt tcaacagctg ttacagtcgt ttcgtgcagg
    12901 ttgtggttca ggctcgcaat tctgattaac aacctgtcca ggtagtatga agaaatacaa
    12961 taagcaggaa tttatattat ctcccccgat ttccgtcatc ggaggagata taactgtcag
    13021 gcaaatatcg tcatcaaata ggcggcaaac agtgccagat gcgctgcgcc attgagcacg
    13081 ttagtacgtc cggtggagaa ggagatatga cacagcacta aagaggccac catcaccacc
    13141 atttctggcg caccaagtgc aaactgcaat tcgttacccg tcataaaggc aattagcgtg
    13201 acgacaggta cggtaagcga aatggttgct aacactgaac caaagaacag attcatcgcg
    13261 cgctggacct ggttgttcaa cactgctttt aacgcaccta aaccttccgg cgacagaatc
    13321 aacagagcca ccaggaagcc agtaaaggcg acaggggcat tcatgctgtc gagcaatgtc
    13381 tccagcgggc tggcgttcat tttggtcacc gcaataacgg caatcagatg gacaatcaac
    13441 cagatagcat gccacaggct gctatgggca gacggtttac cgtgatgcgg gtcgtcgtca
    13501 tcactgtcat cttcgtgctc gtagacaaac aaactttgat gcgttttggt ctggatcagc
    13561 aaaaatacgc catacattgc cgcagaaatt aatgctacca gtaacgcctg accggttgaa
    13621 aaattcgccg caggcagagc cattggaaat accagtacga ttatcgccag ggggaacagc
    13681 gcaattaaat actgcttgat accaaacaga ttcatatatt gggtggcaaa cttgcgtccg
    13741 cccaacaata atgaaaagcc aaccagccca ccggtaacaa tcataatgat tgaatagagc
    13801 gtatcacgca ttagcgttgg cgcggcgtcg ccggttgcca ttaaagctga aatcaaactg
    13861 acttcaagaa taaccactga aaggctaaga ataagcgaac cgtaaggctc tcccaggcga
    13921 tgggctaata cgtccgcatg acggacaaca ctaaaggcgc tacttaaaat accaataaga
    13981 gcaagaagat tgatggcaat gaccactggt agtgtctggc tgcttcccca caggaacagc
    14041 actaccagcg ccagaaccgg gaaaataagc gaagtctcct tgtggcgggt ttttaccgcc
    14101 tcttgagcat ttgacattat ggttatccct ttgcagatga atttatcgaa aatgtaaaaa
    14161 ataggtagga aaaataacag aaagtgtctg gatatcggta acattttacg aatttttacc
    14221 ccgctgtcga ttttttactc atttgggcat aaaaataagt aatacgttta gacaatgttt
    14281 gttttagcac ttcttaagaa gagtctgaca tgaaaattct tatgttttgg caagagtaga
    14341 tattgttgac cacacttaat gttcaacttt gtaaaaggag tcaacgatgc cgtataaaac
    14401 gaaaagcgat ctgccggaaa gcgtaaagca cgtgcttccg tctcatgccc aggatatcta
    14461 taaagaagcg ttcaacagcg catgggatca atataaagat aaagaagatc ggcgtgatga
    14521 cgccagtcgc gaagaaacag cgcataaagt ggcctgggct gctgtgaagc atgaatatgc
    14581 caaaggggat gatgataaat ggcataaaaa atcgtaaaac cggtagctta gttaaagcta
    14641 ttcgtgcggt gttgccttgc aagtggtccg tggattgcat attgtcccgt tagtggtttc
    14701 aaaatgagca gtaaaaatgt ccggaagaca ccaaaaagtt gtcgcaggga agtatgcagt
    14761 ggcggaggtg taaggtgata acgcgtgatt tcttgatgaa tgccgattgt aaaacggcat
    14821 ttggtgccat tgaagaatca ctcttatggt cagcagaaca acgggcggct tcgctggcgg
    14881 cgacgctggc ttgtcgacct gatgagggac cggtgtggat cttcggttat ggatcgttga
    14941 tgtggaatcc ggcactggag tttaccgagt cgtgcaccgg tacactggtt ggatggcatc
    15001 gtgcgttttg cctgcgcctg accgccgggc gcgggactgc gcaccagccg ggacggatgc
    15061 ttgcactgaa agagggcgga cgcaccacag gcgtcgccta tcgactgcca gaagagacgc
    15121 tggagcagga actaaccctg ttgtggaagc gcgagatgat taccggctgt tatctgccaa
    15181 cctggtgtca gcttgatctt gatgatggac gcacagtaaa cgccattgtg tttattatgg
    15241 acccgcgaca tccagaatat gaatctgaca ctcgcgctca ggtcatcgcg ccgttgattg
    15301 cggcggcgag cggtccactg ggaaccaacg cacaatacct gttttcactg gaacaggagc
    15361 ttatcaaact gggaatgcag gacgatgggc tgaatgattt gctggtatcg gtaaaaaaac
    15421 tgctggcgga gaattatccg gatggtgtgt tacggccggg actcgcctga gtaaacttcc
    15481 ctcatagtgg ggcgtcagac gccccctcaa acattaaaat gtgagcactt tatcggctga
    15541 cagcgtccat tgcgccagtt ccacaagcgt accgatttcc accccatcaa tcagaggaag
    15601 tgtactaatc ccgcgcccgt cggtacaggt tttgcacaat ttcaccggta cattctgagc
    15661 ggtaaggatc tccagcattt gctgaatgtt gtagccttcc cctggttttt gcccgcgcaa
    15721 cccggctgtg accgcatcag acatcaggaa cagacgcaga tccagattgc tctcctgctc
    15781 tcgtaacgca atggccagcc gcaagctgtt aaataaggat tcgctcccgt aaggtgcgcc
    15841 attggcaacg atcacgattt tttgcattat ttactcctgt attcagggaa ttagacactc
    15901 atcttctatc ttactgcttc tgcagcgtct gaccaatcgg tcacattttt aaggattttc
    15961 ctgaaagcgc gagaaaatac gacaaaagtt gccagtaatc gttattcttt agggctatgg
    16021 tttttcattt tttaccggaa gttaccgacg ttttgagccg tttcgttcct cgcattattc
    16081 cgttttattt actcttgctg gcggcaggcg gtacagctaa cgcacaatct accttcgagc
    16141 aaaaagcggc aaatcccttt gataataaca atgatggtct gccggattta ggcatggcac
    16201 ctgaaaatca tgatggggaa aaacactttg ctgaaattgt gaaagatttc ggcgaaacca
    16261 gtatgaatga taacgggctg gatactggcg agcaggcaaa agctttcgca ttgggaaaag
    16321 tccgcgacgc gtttagtcaa caggttaatc agcacgtaga gtcgtggcta tcagcgtggg
    16381 gaaatgccag tgttgacgtc aaagtggata acgaaggaca tttcaccggc agtcgtggaa
    16441 gctggtttgt gccgttacaa gataatgatc gttatctcac ctggagccag cttggtctta
    16501 ctcagcagga tgatgggctg gtgagcaatg tgggcgttgg gcaacgctgg gcgcgcggca
    16561 gctggctggt gggttataac actttttatg acaacttgct ggacgaaaat cttcagcgag
    16621 cgggcttcgg tgccgaagcg tggggcgaat atttgcgact atcggcaaac ttttatcagc
    16681 cgtttgctgc atggcatgaa cagacagcca cgcaggaaca acggatggcg cgcgggtacg
    16741 acctgacagc ccggatgcgc atgccgtttt atcaacacct caataccagt gtcagcgtag
    16801 aacagtattt tggtgatcgt gttgatttgt ttaactctgg tacgggttat cacaatcccg
    16861 tcgcgttgag tctgggatta aattataccc cagtgccatt agtcactgtg acggcccagc
    16921 ataaacaggg tgaaagtggc gaaaatcaaa ataacctcgg gctgaatctt aattaccgct
    16981 ttggtgtacc gctcaaaaaa caactttctg cgggcgaggt tgccgaaagt cagtcgttac
    17041 gtggtagtcg ctatgacaat ccgcagcgaa ataatctacc gactcttgag taccgacagc
    17101 gaaaaacgtt aacggtgttt ctggcgacac cgccgtggga tctaaaacct ggcgaaacag
    17161 tgccgctgaa attacaaatc cgcagtcgtt acggtattcg gcaactgatt tggcagggcg
    17221 atacgcagat attaagtttg acgccgggcg cacaagccaa cagtgaggag ggctggacgc
    17281 tgatcatgcc tgactggcaa aacggggaag gcgcaagcaa tcactggcga ttgtcagtgg
    17341 tggtggaaga taaccagggg cagcgtgtct cctccaatga gatcacgcta acgcttgtcg
    17401 aaccgttcga cgcattgtca aacgacgaac tgcgctggga accgtaatca gaaaatgcgc
    17461 tcctgatgca cccataccgc agcttccacg cgagacttta gcttcatttt cttcagcatg
    17521 tgcttgacgt gaacttttac tgtgctttcg gtgatgtcca ggcggcgggc aatcatcttg
    17581 ttcggcaaac cctgggcaat tagcttgaga atatcgcgct cgcgtggggt taactggtta
    17641 acatcgcgct cagtagtggc acggttagcg cgcaagctgg cggccagaac aggcgttaat
    17701 gcttcgctta ataccatttc gccagcagct gcctgatgta acgctttcag cagatcttcc
    17761 ggttccatat cttttaacag atagccatcc gcgccgcgtt tcagtgcggt gaccacatct
    17821 tcttcatggt tagagacgct gaataccaca atgcgcccgg agagggactt ttcgcgcagt
    17881 ttatccagcg tttccagacc gttcatgccg ggcatattga gatctaacag gatcagatcg
    17941 ggatcaagag actccgccag ttcaataccc tgttcgccat tactcgcttc gccaaccacg
    18001 gtgatatctg gtgccatact gataagctgt tttacgccag ttcgcagcat cgggtgatcg
    18061 tcaatcagca ggatagtagc cggttcctga ttactcatgg gtatctcctt ggacgtctgt
    18121 gaaagttttt tcgggaataa aggtgaccac cacttcggtg ccacctgatt cacgacggcg
    18181 gacgcggcaa tcgccccgta aactttgcgc acggtcgcgc attattatca tgccgtagtg
    18241 attgctgcgg atggcatttt caggcacgcc gcagccgtta tcctggacgg tcagtttgac
    18301 ctgattatcg ttttgcgcca ctgtcaccac gacctcactc gcttgcgaat gtttgagggc
    18361 gttacttaat gcctcacggg caatttgcaa cagatggatt gcctgatgcg aaggcaccag
    18421 acgaggcggc aattgataat ccagcttcac cgggaagcca aacctggcgc tgtactcttc
    18481 gcaactcgcc tccagcgccg gacgtaatcc aggctcggtg agctgcaagc ggaatgtggt
    18541 gagcaattca cgcaactgcg cccaggatgc attcagttcg ttacggatct gacttaacag
    18601 ttcgcggctg ctttctggca gcgcatcgcc ctgcatctgt aaacaactca cctgcatctt
    18661 catgcaagag agagattggg caatagaatc atgcagttcg cgcgcaatgg tggcacgctc
    18721 ttccatcacg atcaactgtt gctgacgttc ctgatggcga tccagcgcaa gcgtggcggt
    18781 gagttgttca accagggtat ccaccagttg ttgttgatca tgactaagat gacgcccctg
    18841 cggcagggtt gccagcaaaa taccgtactg cgtatgagag tcagccagcc gccacttcag
    18901 ggttgtgccg cgatcgccaa cggggagtac accgcgcggg cagagctgac agcctttatc
    18961 atcgcaggtc atatctggct ggcaggtaaa ctcctgatga ttctcttcat catcagtgtc
    19021 atacacccgc agttcgatat cacgtagcag ggttaaattc tgtaagccgt tgagtacagg
    19081 tgacaggcgt tcacacagcg gggcgcggga atgcaaacgg cggttggcct gccataaaaa
    19141 agagaggatc tgatttttat gttccagccc ggcggttttc tcctgaaccc gctgctcaag
    19201 tacggcgtaa ctttccgcca gttctgcaga catattgttc aacgcagttc caagcatagc
    19261 catttcgttg cgcccgctga tgttcgcgcg ttgggtaaaa tcgcgatggc tgacggcact
    19321 cgccattgcc aacaactgcc gccacggttg tagcagtcgc gcccgcaacc agataatagt
    19381 gaacaccagt aaaagtgcca taaataccgc cattacccga tggaccagca ccactgtctc
    19441 aatgcgcatt tccgtggtgc ggtcaaaacc agataccagt tgatcaagcc cggcaacaaa
    19501 ctggctgaca tccgctgaca ccgtttcgcg gttttgtgca cgcattagcg cagggatcag
    19561 ttcattacgc cagtaatctt gtaaaccctg taattgcgcc agttgtccgt ctcgttctgc
    19621 tgctcgggtt aactcggcgc taaatgctgt ttgttccatc tctttaatta agggcttgtc
    19681 tttctcgctt aatggcactg ctgccaacag acggtaactt tgcatgcgca gcgatcccgc
    19741 tttgttgatc gcatgggcgc tgccctgaac gccttgcacc agccagccag aaactgccat
    19801 ccctgccagt ccaatagcag tagaaagcaa cacaataagc gcaacctgat taaccagggt
    19861 gagcggagag agacaacgtt taagcatgca aacctcttcc ttcaggcttt aaatgagcaa
    19921 taaccttaat gaatgtgacg atacattctg gaatggcagt attctcggct attggctgaa
    19981 gtatacccat acccggaaag agttactcct tatttgccgc gtggttagtc gctttacatc
    20041 ggtaagggta gggattttac agcaccgtga aaaatctcat aatttttatg aaatcactgt
    20101 actcactatg ggtaatgata aatatcaatg atagataaag ttatcttatc gtttgattta
    20161 catcaaattg cctttagcta cagacactaa ggtggcagac atcgaaacga gtatcagagg
    20221 tgtctatgag tcactcatcc gcccccgaaa gggctactgg agctgtcatt acagattggc
    20281 gaccggaaga tcctgcgttc tggcaacaac gcggtcaacg tattgccagc cgcaacctgt
    20341 ggatttccgt tccctgtctg ctgctggcgt tttgcgtatg gatgttgttc agcgctgttg
    20401 cggtgaactt accgaaagtc ggttttaatt ttacgaccga tcagctattt atgttgacgg
    20461 cgctgccttc ggtttctggc gcgttattac gtgttccata ctcctttatg gttcctatct
    20521 tcggtggtcg tcgctggacg gcgttcagca ctggtattct gattattcct tgcgtctggc
    20581 tgggttttgc cgtgcaggat acctccacgc cttatagcgt cttcatcatc atctctctgc
    20641 tgtgcggctt tgctggcgcg aacttcgcat ccagtatggc aaacatcagc ttcttctttc
    20701 cgaaacagaa gcagggtggc gcgctgggtc tgaatggtgg tctgggcaac atgggcgtca
    20761 gcgtcatgca gttggttgct ccgctggtgg tatcactgtc gattttcgca gtatttggta
    20821 gccagggtgt caaacagccg gatgggactg agctgtatct ggcgaatgcg tcctgggtat
    20881 gggtgccgtt ccttgccatc ttcaccattg cggcgtggtt tggcatgaac gatcttgcta
    20941 cctcgaaagc ctccatcaag gagcagttgc cggtactcaa acggggtcat ctgtggatta
    21001 tgagcctgct gtatctggca accttcggct ccttcatcgg cttctccgcg ggctttgcga
    21061 tgctgtcaaa aacgcagttc ccggatgttc agattctgca atacgctttc ttcgggccgt
    21121 ttattggtgc gctggcgcgt tctgcaggtg gtgcattatc tgaccgtctg ggcagaactc
    21181 gtgtcacact ggtgaacttt atcctgatgg cgattttcag cggcctgctg ttcctgacct
    21241 taccgactga cggacagggc ggaagcttca tggcgttctt cgcagtcttc ctggcgctgt
    21301 tcctgacagc tgggctgggt agtggttcca cttttcagat gatttccgtg atcttccgta
    21361 aactgacaat ggatcgcgtg aaagcagaag ggggctctga cgaacgtgcg atgcgtgaag
    21421 cggcaaccga cacggcggcg gcgctgggtt tcatctctgc gattggcgcg attggtggct
    21481 tctttatccc gaaagcgttc ggtagctcgc tggcattaac gggttcgcca gtcggcgcaa
    21541 tgaaagtatt tttgattttc tatatcgcct gcgtagtgat tacctgggcg gtatatggtc
    21601 ggcattctaa aaaataaatc ttaatatttg attatccttt gcgcggcatg atgtcgcgct
    21661 ttttttacct gtcatttagt tacaacatcc ttatgaagtg tggcgtattt cgccggtttt
    21721 cattaagagc cattaatatg ttacccatgg ggaatactcc ttaataccca tctgcataaa
    21781 aatctcaata gtctaaataa ctatggatgc aaaatatctt aatttacagt gatttatgtt
    21841 gaatttatta attatcctta aggggtatct taggaattta ctttattttt catccccatc
    21901 actcttgatc gttatcaatt cccacgctgt ttcagagcgt taccttgccc ttaaacatta
    21961 gcaatgtcga tttatcagag agccgacagg ctcccacagg agaaaaccga tgagtaaatt
    22021 cctggaccgg tttcgctact tcaaacagaa gggcgaaacc tttgccgatg ggcatggcca
    22081 gcttctcaat accaaccgtg actgggagga tggatatcgc cagcgttggc agcatgacaa
    22141 aatcgtccgc tctacccatg gggtaaactg caccggctcc tgcagctgga aaatctacgt
    22201 taaaaacggt ctggtcacct gggaaaccca gcagactgac tatccgcgta ctcgcccgga
    22261 tctgccaaac catgaacctc gcggctgccc gcgcggtgcc agctactcct ggtatcttta
    22321 cagcgccaac cgtctgaaat acccgatgat gcgcaaacgc ctgatgaaaa tgtggcgtga
    22381 agcgaaggcg ctgcatagcg atccggttga ggcatgggct tctatcattg aagacgccga
    22441 taaagcgaaa agctttaagc aggcgcgtgg acgcggtggt tttgttcgtt cttcctggca
    22501 ggaagtgaac gaactgatcg ccgcatctaa cgtttacacc atcaaaaact acggcccgga
    22561 ccgtgtcgct ggcttctcgc caattccggc aatgtcgatg gtttcttacg cgtcgggtgc
    22621 gcgttatctc tcgctgcttg gcggtacttg cttaagcttc tacgactggt actgcgatct
    22681 gcctcctgcg tctccacaaa cctggggcga gcaaactgac gtaccggaat ctgctgactg
    22741 gtacaactcc agctacatca tcgcctgggg gtcaaacgtg ccgcagacgc gtactccgga
    22801 tgctcacttc tttactgaag tgcgttacaa aggcaccaaa accgttgccg tcacgccgga
    22861 ctacgctgaa atcgccaaac tgtgcgatct gtggctggca ccgaagcagg gcaccgacgc
    22921 agcaatggcg ctggcgatgg gtcacgtgat gctgcgtgaa ttccacctcg acaacccaag
    22981 ccagtatttc accgactatg tgcgtcgtta taccgacatg ccaatgctgg tgatgctgga
    23041 agagcgcgac ggttactacg ctgcgggtcg tatgctgcgc gctgctgatc tggtggatgc
    23101 gctgggccag gaaaacaatc cggaatggaa aactgtcgcc tttaatacca atggagaaat
    23161 ggttgcgccg aacggttcta ttggcttccg ctggggcgag aagggcaaat ggaatcttga
    23221 gcagcgcgac ggcaaaactg gcgaagaaac cgagctgcaa ctgagcctga tgggtagcca
    23281 ggatgagatc gctgaagtgg gcttcccata ctttggtggc gacggcacgg aacacttcaa
    23341 caaagtggaa ctggaaaacg tgctgctgca caagctgccg gtgaaacgcc tgcaactggc
    23401 tgatggcagc actgctctgg tgaccaccgt ttatgatctg acgttggcaa actacggtct
    23461 ggaacgtggc ctgaacgacg ttaactgcgc aaccagctat gacgacgtga aagcctacac
    23521 ccctgcatgg gccgagcaga tcactggcgt ttctcgcagc cagattatcc gcattgcccg
    23581 tgaatttgcc gataacgctg acaagacgca cggtcgttcg atgattatcg tcggtgcggg
    23641 gctgaaccac tggtatcacc tcgatatgaa ctatcgtggt ctgatcaaca tgctgatttt
    23701 ctgcggctgt gtaggtcaga gcggtggtgg ctgggcgcac tatgtaggtc aggaaaaact
    23761 gcgtccgcaa accggctggc agccgctggc gtttgctctt gactggcagc gtccggcgcg
    23821 tcacatgaac agcacctctt atttctataa ccactccagc cagtggcgtt atgaaaccgt
    23881 gaccgcagaa gagttgttgt cgccgatggc ggataaatcc cgttataccg gacatctgat
    23941 cgactttaac gtccgtgcag aacgtatggg ctggctgccg tctgcgccgc agttaggcac
    24001 taacccgctg actatcgctc gcgaagcaga aaaagccggg atgaatccgg tggactatac
    24061 ggtgaaatcc ctgaaagagg gttccatccg ttttgcggca gaacaaccag aaaacggtaa
    24121 aaaccacccg cgtaacctgt tcatctggcg ttctaacctg ctcggttctt ccggtaaagg
    24181 tcatgagttt atgctcaagt acctgctggg gacggagcac ggtatccagg gtaaagacct
    24241 gggtcagcag ggcggcgtga agccggaaga agtggactgg caggacaacg gtctggaagg
    24301 caagttggat ctggtggtga cgctggactt ccgtctgtcg agcacctgtc tctattccga
    24361 catcattttg ccgacggcga cctggtacga aaaagacgac atgaatactt cggatatgca
    24421 tccgtttatt cacccgctgt ctgctgcggt cgatccggcc tgggaagcga aaagcgactg
    24481 ggaaatctac aaagccatcg cgaagaaatt ctccgaagtg tgcgttggtc atctgggcaa
    24541 agaaaccgac atcgtcacgc tgcctatcca gcacgactct gccgctgaac tggcgcaagc
    24601 gctggatgtg aaagactgga aaaaaggcga atgtgacctg atcccaggta aaaccgcacc
    24661 gcacattatg gttgtagagc gcgattatcc ggcaacttac gaacgcttta cctctatcgg
    24721 cccgctgatg gagaaaatcg gtaacggcgg taaagggatt gcctggaaca cccagagcga
    24781 gatggatctg ctgcgtaagc tcaactacac caaagcggaa ggtccggcga aaggccagcc
    24841 gatgctcaac accgcaattg atgcagcaga gatgatcctg acgctggcac cagaaaccaa
    24901 cggccaggtg gccgtgaaag cctgggcggc attgagtgag tttaccggtc gtgaccatac
    24961 gcatctggcg ctgaataaag aagacgagaa gatccgcttc cgtgacattc aggcgcagcc
    25021 gcgcaaaatc atctccagcc cgacctggtc tggccttgaa gatgaacatg tttcttacaa
    25081 cgccggttac accaacgttc acgagctgat cccgtggcgt acgctctctg gccgtcagca
    25141 gctgtatcag gatcatcagt ggatgcgtga tttcggtgaa agcctgctgg tttatcgtcc
    25201 gccgatcgac acccgttcgg tgaaagaagt gatgggccag aaatctaacg gtaatccgga
    25261 aaaagcgctc aacttcctga caccgcacca gaagtggggt atccactcca cctacagcga
    25321 caacctgctg atgctgactt taggtcgcgg tggtccggtg gtctggttga gtgaagccga
    25381 tgccaaagag ctgggtatcg ccgacaacga ctggattgaa gtcttcaaca gcaacggtgc
    25441 attgactgcc cgtgcggttg tcagccagcg tgttccggca gggatgacca tgatgtacca
    25501 cgcgcaggaa cgtatcgtta acctgccggg ttcagaaatc accagccagc gcggtggtat
    25561 tcataactcg gtcacccgta tcacgccgaa accgacgcat atgatcggcg gctatgccca
    25621 tctggcatac ggctttaact actacggcac tgtaggttct aaccgcgatg aatttgttgt
    25681 agtgcgtaag atgaagaaca ttgactggtt agatggcgaa ggcaatgacc aggtacagga
    25741 gagcgtaaaa tgaaaattcg ttcacaagtc ggcatggtgc tgaatctgga taagtgcatc
    25801 ggttgccaca cctgttcagt gacctgtaaa aacgtctgga ccagccgtga aggcgtggaa
    25861 tacgcgtggt tcaacaacgt ggaaaccaag ccgggccagg gcttcccgac agactgggaa
    25921 aaccaggaaa aatacaaagg cggctggatc cgtaaaatca acggcaaact gcaaccgcgc
    25981 atgggtaacc gcgccatgct gctgggtaaa attttcgcta acccgcatct gccggggatc
    26041 gacgattatt acgagccgtt cgattttgac tatcagaacc tgcataccgc gccggaaggc
    26101 agcaaatcgc agccgattgc ccgtccacgt tcgctgatta ccggcgaacg gatggcgaaa
    26161 atcgaaaaag ggccaaactg ggaagatgac ctgggcggcg agtttgacaa actggcgaaa
    26221 gacaagaact tcgacaacat ccagaaggcg atgtatagcc agttcgaaaa caccttcatg
    26281 atgtatttgc cacgcctgtg cgaacactgc ctgaacccgg catgtgtggc gacctgcccg
    26341 agcggtgcga tttacaagcg tgaagaagat ggcatcgtcc tgatcgacca ggacaaatgc
    26401 cgtggctggc gtatgtgcat caccggatgc ccgtacaaaa aaatctactt caactggaag
    26461 agcggtaagt ctgagaagtg catcttctgc tatccgcgta ttgaagccgg tcagccgacg
    26521 gtgtgctctg aaacctgtgt cggtcgtatc cgttatcttg gcgtgctgct gtacgatgcc
    26581 gacgcgattg aacgtgcagc cagcaccgag aacgagaaag atctctacca gcgtcagctg
    26641 gacgtgttcc tcgatccgaa cgatccgaag gtcatcgagc aggcgattaa agacggtatt
    26701 ccgctgagcg ttattgaagc cgcacagcag tcgccggttt ataaaatggc gatggaatgg
    26761 aaactggcgc tgccgctgca tccggaatat cgcacgctgc cgatggtctg gtatgtgcca
    26821 cctctgtcgc cgattcagtc tgcggcagat gcgggtgagc tgggcagcaa tggcattctg
    26881 ccagacgtcg aaagcctgcg tattccggta cagtatctgg cgaatctgct gaccgcaggt
    26941 gataccaaac cggtactgcg cgcgctgaaa cgtatgctgg cgatgcgtca ttataaacgt
    27001 gctgaaaccg ttgacggtaa agttgatacc cgtgcgctgg aagaggtcgg tctgaccgaa
    27061 gcccaggcac aggagatgta ccgttatctg gcgattgcta actacgaaga tcgctttgtg
    27121 gtgccgagca gtcatcgtga actggcgcgg gaagccttcc cggagaaaaa tggctgcggc
    27181 tttacctttg gtgatggctg ccacggctca gataccaaat tcaatctgtt taacagccgt
    27241 cgtatcgatg ccatcgacgt gaccagtaaa acggagccgc acccatgatc gaactcgtga
    27301 ttgtatcgcg tctccttgaa tatccggatg ctgccttatg gcagcatcaa caagagatgt
    27361 ttgaggcgat tgccgcgtcg aaaaatctgc caaaagagga tgcccatgcg ctgggcattt
    27421 tcctgcgcga tttaacgacg atggatccgc tcgatgccca ggcgcagtac agcgaactgt
    27481 tcgaccgtgg ccgcgccacg tcactgttgc tatttgaaca tgttcacggt gaatcccgtg
    27541 accgcggcca ggcgatggtg gatctgctgg cgcagtacga gcagcacggc ttgcagttaa
    27601 acagccgcga attgcccgac catctgccgc tgtatctgga gtacctggcg cagctgccgc
    27661 aaagcgaagc cgtggagggt ttgaaagata ttgcgccgat tctggcattg ctgagcgcgc
    27721 gtctgcaaca gcgtgaaagc cgttatgccg tgctgtttga tctgctgctg aaactggcga
    27781 ataccgccat cgacagcgac aaagtggcgg aaaaaattgc cgatgaagcg cgcgacgata
    27841 cgccgcaggc gctggatgca gtctgggaag aagagcaggt taaattcttt gctgaaaaag
    27901 gctgcggtga ttcagcaatc actgctcatc agcgtcgctt tgccggtgcc gtcgcgccgc
    27961 aatatctgaa tatcaccacc ggaggacagc actaatgcaa ttcctgaata tgttcttctt
    28021 tgatatctac ccgtacatag ccggggcggt cttcctgatt ggtagctggc tgcgttatga
    28081 ctacgggcag tacacctggc gcgcggcgtc cagccagatg ctggatcgca aagggatgaa
    28141 cctggcgtcg aacctgttcc atatcggtat tctggggatt tttgttggtc acttcttcgg
    28201 tatgctgacg ccgcactgga tgtatgaagc ctggctgccg attgaagtga aacagaaaat
    28261 ggcgatgttt gctggtggtg ccagcggtgt gctgtgtctg attggcggcg tgctgttgct
    28321 gaagcgtcgt ctgttcagcc cacgcgtgcg tgcaaccact accggagcgg atatcctgat
    28381 cctgtcgctg ctcgttatcc agtgcgcgct gggcctgttg accattccgt tctccgccca
    28441 acatatggac ggtagcgaga tgatgaaact ggttggctgg gcgcagtcgg tagtgacctt
    28501 ccacggtggc gcttctcaac accttgatgg tgtggcgttt atcttccgtc tgcacctggt
    28561 gttggggatg acgttattcc tgctgttccc gttctcgcgt ctggtgcaca tctggagcgt
    28621 accggtggag tatctgacac gtaagtatca gctggtgcgc gctcgtcact aagcgaattt
    28681 tagtttgcat agaccccgct tcggcggggt ttttttatgg gcacggtacg gggtgagttg
    28741 tcggatgcgc ttcgcttatc cgacctacag gggaggatat tgtaggtccg gtaaacgcgg
    28801 tgccattggg cagaattagc tcggagtaac aggttttgag tatttacagg aagtgatggt
    28861 ggtggaggaa ggattactca gcgctgcgcg cttcgccctt cgggtcgttg cctgcggcca
    28921 cgctctctcg ctggcgctcg agtcgaaccc tggtcgaagc ttctcatcct tccccgcttg
    28981 ggcagaatat ttaatcgcgg attcgtttga gagttcacgg acttttgaaa gtgatggtgg
    29041 tgggggaagg attcgaacct tcgaagtcga tgacggcaga tttacagtct gctccctttg
    29101 gccgctcggg aaccccacca cggggtaatg ctttttacta gcctgctccc ttatcgggaa
    29161 gcggggcgca tcatatcaaa tgacgcgccg ctgtaaagtg ttacgttgag aaaaatgaac
    29221 tggttgcgta attttcaccc gtaacggatt aaaggtaacc agttattttt gctggcgatt
    29281 aaagaataat cgtccgatta ccgtaaacaa agacgcgctg cgccagtact ttgtagagtg
    29341 cgcgacttaa gacgtttttc tcgacgtcac gacctgcgcg catcatatct tcagctgtgt
    29401 aggtatgatc gacatgaata acgtcctgca tgatgattgg accttcgtcc agattgtcat
    29461 tcacatagtg agcggtagcg ccaataatct tcacgccacg ttcataggcc tggtgataag
    29521 gacgtgcgcc gataaacgct ggcaggaatg aatggtgaat attgatgatc ttattcggga
    29581 agcgtgccac aaattctggc gttaataccc gcatatactt cgccagcacc acgtagtcag
    29641 gctgataagc atcaatggca tccgccatct tttgatcgtg ctcgttgcgg cttaaccctt
    29701 catggcttac cagctcaaac ggaatatcaa aacgctcaac cagagaacgt aaagtatcgt
    29761 ggttaccaat aactgccgcg atttcgacat ccaggccgcc gtaattggct ttcatcaaca
    29821 aatcgccaag gcaatgcgcc tctttagtga ccagaatcac tatccggcga cgaccggcag
    29881 gattcagctc acgtacggag ccttctggca atgcgctgtc gagatccgcc agcagggtgg
    29941 aatcattaaa aattccttcc agttccgtgc gcataaaaaa gcgcccggta cggtgatcaa
    30001 caaattcatt gttctgtacg atatttaact cgtgcttgta gcaaatattg gtaatacgtg
    30061 cgatcagacc tttttggtcc ggacaaatag tacgcagaac tttacgttgg agtgaatgca
    30121 ttgctggaaa aaccttgttg agagtgtttg ctaaaccgta ccgtcaacca ttattggccg
    30181 cagcactttt taaatttttt acctgaacca caagggcagg gatcgttgcg gccaaactgc
    30241 ggacgtgtac cgtcaatata gtaccattga ccgttttctt tcaaaaatcg cgaacgttca
    30301 attattgcac ctgttttgct accttcggta aatcgcgcaa caaaactgac aaatccgata
    30361 ttgtcgacgt cctgccaaca atgttcaaat acggtcaatc ccaaccattc agtatgcgca
    30421 aatccggcaa tcaattcggc acgtaacgtc gccgctccac aagatggatg ccaggtcttt
    30481 attaaataat ctgcgtcttt catcacaaaa gcgcagtaac gcgaacgcat gagatgttct
    30541 ggatcaggtg caaccttttc accagacaca taagggtggc aacataggct atactcgaca
    30601 gcactaccac agggacaaag ctgagacaca aataatctcc ctggaaacaa taacggcgta
    30661 ttaaccgcct gagtagcact atgttaaccg agcagtagcg atgttgctac gattgcattc
    30721 caggggaatc ttgcgggaat aatgagaaag ataaaaatag ggctggcgct gggatctggc
    30781 gcggcgagag gttggtcgca tattggcgtt attaatgcgc taaaaaaagt gggtattgaa
    30841 attgatatcg ttgcaggatg ttcaattggt tcgctggtgg gcgctgccta tgcatgcgat
    30901 cgactatctg cgctggaaga ttgggtgacc tctttcagtt attgggatgt tttacgcctg
    30961 atggatctct cctggcagcg cggtgggtta ctgcgcggcg agcgtgtctt caatcaatat
    31021 cgcgaaataa tgccggaaac agagatcgaa aattgttccc gtcgcttcgc ggctgttgcc
    31081 accaatttaa gtactgggcg cgaattatgg tttactgaag gcgatctcca tcttgctatt
    31141 cgtgcatcat gcagtattcc aggactaatg gcacccgttg cgcataacgg ctactggctg
    31201 gttgatggcg ctgtagttaa cccaattcct atttccctca cgcgtgcatt gggggctgat
    31261 attgtgatag cggtcgacct gcagcacgat gctcatttga tgcaacaaga tttgctctcc
    31321 tttaatgtca gtgaagaaaa tagcgagaat ggtgattctc tgccgtggca tgcgcgtctg
    31381 aaagaaaggt taggcagcat aacgacacgt cgggcggtga cagcgccaac ggcaacagag
    31441 attatgacca cttctatcca ggtgctggag aaccgcctta aaaggaaccg catggcaggt
    31501 gatccgcccg atattctgat tcaacctgtt tgcccgcaaa tatctacgct tgatttccat
    31561 cgcgcgcacg ctgccattgc ggccgggcag ctggcagtgg aaaagaaaat ggacgaactt
    31621 ttgccgttgg tacgcaccaa catttgacca gaatttttat ctacacttaa gttaattctg
    31681 acaggcgcag gtggcaatag catgccacta ttgagtaaag ccagtcaggg gagagaacat
    31741 gacgcagcca ttggtcggaa aacagattct cattgttgaa gatgagcagg tatttcgctc
    31801 gcttctggat tcgtggtttt cctcattggg agcgacaacg gtactggcgg ctgatggggt
    31861 ggatgccctt gagttgctgg gaggtttcac tccagacctg atgatatgtg atatcgcgat
    31921 gccacgaatg aacgggctta aactgctgga gcatatacgt aacagaggcg accagacccc
    31981 agttctggtg atatctgcca ctgaaaatat ggcagatatt gccaaagcgt tacgtctggg
    32041 cgttgaagat gttttgctga aaccagttaa agatctgaat cgcttgcgcg agatggtttt
    32101 tgcctgtctc tatcccagca tgtttaattc ccgcgttgag gaagaggaaa ggctttttcg
    32161 cgactgggat gcaatggttg ataaccctgc cgcagcggcg aaattattac aggaattgca
    32221 accgccggtt cagcaggtga tttcccattg ccgggttaat tatcgtcaat tggttgccgc
    32281 ggacaaaccc ggcctggtgc ttgatattgc cgcactttcg gaaaacgatc tggcatttta
    32341 ctgccttgat gtcacccgag ctggacataa tggcgtactt gctgccttgt tattacgcgc
    32401 attgtttaac ggattattac aggaacagct tgcacaccaa aatcaacgtt tgccagagtt
    32461 gggcgcgtta ttgaagcagg taaaccattt actccgtcag gccaatctgc cggggcagtt
    32521 tccgctatta gttggctatt atcatcgcga actgaaaaat ctcattctgg tttctgcggg
    32581 tctgaatgcg acgttaaata ccggcgaaca ccaggtgcaa atcagtaatg gtgttccgtt
    32641 aggcacttta ggtaacgctt atttgaatca attgagccag cgatgcgatg cctggcaatg
    32701 ccaaatatgg ggaaccggtg gtcggcttcg cttgatgttg tctgcagaat gagcaaacga
    32761 taacgcgggc taaatttgca ttacctgcta atgtcggctg gtggtactat cgtcgccatt
    32821 cgtataagta attgtcttaa ttatgctaac tcgcctcctt ttcagaactt agccccttcg
    32881 gggtgctgat atactgggat gcgatacaga aatatgaaca cgttcaaaac acgaacagtc
    32941 caggagaatt taaatggctg ccattaatac gaaagtcaaa aaagccgtta tccccgttgc
    33001 gggattagga accaggatgt tgccggcgac gaaagccatc ccgaaagaga tgctgccact
    33061 tgtcgataag ccattaattc aatacgtcgt gaatgaatgt attgcggctg gcattactga
    33121 aattgtgctg gttactcact catctaaaaa ctctattgaa aaccactttg ataccagttt
    33181 tgaactggaa gcaatgctgg aaaaacgtgt aaaacgtcaa ctgcttgatg aagtgcagtc
    33241 tatttgtccg ccgcacgtga ctattatgca agttcgtcag gggctggcga aaggcctggg
    33301 acatgcggta ttgtgtgctc acccggtagt gggtgatgaa cctgtggctg ttattttgcc
    33361 tgacgttatt ctggatgaat atgaatccga tttgtcacag gataacctgg cagagatgat
    33421 ccgccgcttt gatgaaacgg gtcatagcca gatcatggtt gaaccggttg ctgatgttac
    33481 cgcatatggc gttgtggatt gcaaaggcgt tgaattagcg ccgggtgaaa gcgtaccgat
    33541 ggttggtgtg gttgaaaaac caaaagcgga tgttgcgccg tctaatctcg ctattgtggg
    33601 tcgttacgta cttagcgcgg atatttggcc gttgctggca aaaacccctc cgggagctgg
    33661 tgatgaaatt cagctcaccg acgcaattga tatgctgatc gaaaaagaaa cggtggaagc
    33721 ctatcatatg aaagggaaga gccatgactg tggtaataaa ttaggttaca tgcaggcctt
    33781 cgttgaatac ggtattcgtc ataacaccct tggtacggaa tttaaagcct ggcttgaaga
    33841 agagatgggc attaagaagt aacatccgta tcggtgttat ccacgaaacg gcgttgagca
    33901 atcgacgccg tttttttata gcttattctt attaaattgt cttaaaccgg acaataaaaa
    33961 atcccgccga tggcgggatt tttaagcaag tgcaatctac aaaagattat tgcttgatca
    34021 ggaaatcgtc gagggattta ccttgctcat ccattgcttt tttgattaca gctggagtac
    34081 ggccctggcc agtccaggtt ttagtttcgc cgttttcgtc aacgtagcta tattttgccg
    34141 gacgctgagc acgtttagct ttggtgccag atttaacggc agcaaggcta ttcagcagtt
    34201 cgttcgggtc aataccgtca gcgatcagca tttcgcgata ttgctgcagt ttacgagtgc
    34261 gctcttcaac ttcagcagca gccgcgcttt cttcttcgcg acgttcgtta acaacaactt
    34321 ctaatttttc cagcatttct tccagcgttt caagtgtaca ttctcttgcc tgcgcacgaa
    34381 gagtacggat gttgttcaga attttaagtg cttcgctcat tgtagtaatc tcaaacttat
    34441 attggggtgg tttgttgagg taataataga gccttaaatt cagttgtgca atagccagga
    34501 atgtaaggaa ttcaaaattg ttctttattt tgtgccgcca ataaatatct tttcataaaa
    34561 ttagccagaa aagacgcggc atatagccct atttgcgccg atgatttcgc agcacgtgag
    34621 gttaaaactt cctgattcat gtcacatttt atggggagat tatcgtaggc tgacgacctt
    34681 tcagtcttct gtattagtcg tgtttacaag aattccctat taagcgaatg atgaaaagta
    34741 gaacagtcgc aataagagca gggacttagt attgcactat ctcctggagg tcaacagagg
    34801 gctattactt gcgcaacagg ttaaagattg tgaatagtta ccagcagtca tttacccgct
    34861 tataacaagc gaggcagttg taatgatagc tcagaaggat tatgcaaggc ttcgtaaggg
    34921 agaacgcata taccacttct gtgcatactg ttgagctgaa aaactgacga attatgataa
    34981 actccagcca actttaattc atatcattga gggcctgtgg ctgatggcac agctatattt
    35041 ctactattcc gcaatgaatg cgggtaagtc tacagcattg ttgcaatctt catacaatta
    35101 ccaggaacgc ggcatgcgca ctgtcgtata tacggcagaa attgatgatc gctttggtgc
    35161 cgggaaagtc agctcgcgta taggtttgtc atcgcctgca aaattattta accaaaattc
    35221 atcattattt gatgagattc gtgcggaaca tgaacagcag gcaattcatt gcgtactggt
    35281 tgatgaatgt cagtttttaa ccagacaaca agtatatgaa ttatcggagg ttgtcgatca
    35341 actcgatata cccgtacttt gttatggttt acgtaccgat tttcgaggtg aattatttat
    35401 tggcagccaa tacttactgg catggtccga caaactggtt gaattaaaaa ccatctgttt
    35461 ttgtggccgc aaagcaagca tggtgctgcg tcttgatcaa gcaggcagac cttataacga
    35521 aggtgagcag gtggttattg gtggtaatga acgctacgtt tctgtatgcc gtaaacacta
    35581 taaagaagcg ttagaagtcg gctcattaac ggctattcag gaaaggcatc gccacgatta
    35641 ataagaattt ctttactgac agggtgagca gggcgctttt atcctgtcag ttcgttttac
    35701 tcactttatc cggtgtatat acccttctcg acagttcttt aacgccgcta tacccctcac
    35761 agagcgcttt tacagtgacg ttgacttgtg acctgtaaag tacaatatcg ctgtgtgtag
    35821 gcgttataca tcgccgtaaa tatgatgcag gcaaatgctg tcggtttatg ggaaagttgc
    35881 tttgggtaaa aaaaacggcc ccagaaggga taatgccagt cagttaagca actgactggc
    35941 tctttttcgg ggctgtgggg tatttccagg gcctctcctt taccactctc gggaaggcac
    36001 tttcccttct tgtcggtaat ttcacaagtt gtcccatact tgcaagatcg cgcatcagct
    36061 ccggtatacg tcccggtgaa gcgccctgca atgtcatcag cattctcatc accattccgc
    36121 atgattctga gaaactcagt tgattcggcc agtaaccttt cagatgttcc gccattttaa
    36181 tcatctgata tctcaccaga ttataagcca gtaagacacc ccacagctct tgctccacaa
    36241 gctccggctt tttacttctc agcgtcagcc tgctcagttg catcgtctgt tttatctccc
    36301 tgtatcccag ttcgatttcc cagcgatgac tgtacagatc cgccatttct cctccgggga
    36361 agcgcatggc gtccgtcatc gacgtcagca gatggcagac ttttcctttg cgcgtcacgg
    36421 tcagcaggcg ggctgtcact tcatttccca gtcccggcca c
//
LOCUS       DAZGQL010000049        36454 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.049, whole genome shotgun sequence.
ACCESSION   DAZGQL010000049 DAZGQL010000000
VERSION     DAZGQL010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36454
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.049"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            44..760
                     /gene="yeeN"
                     /locus_tag="ACHKIR_002657"
     CDS             44..760
                     /gene="yeeN"
                     /locus_tag="ACHKIR_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO9990375.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
     gene            complement(1213..1288)
                     /locus_tag="ACHKIR_002658"
     tRNA            complement(1213..1288)
                     /locus_tag="ACHKIR_002658"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1253..1255),aa:Asn,seq:gtt)
     gene            complement(1389..2876)
                     /gene="yeeO"
                     /locus_tag="ACHKIR_002659"
     CDS             complement(1389..2876)
                     /gene="yeeO"
                     /locus_tag="ACHKIR_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HFO9990376.1"
                     /translation="MWFHFLQLRSALNISSALRQVVHGTRWHSKRKSYKVLFWREITP
                     LAVPIFMENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVVMAFFAAIDLGTTVV
                     VAFSLGKRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALA
                     LTYLELTVLSYPAAAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGILIYGLFSW
                     PGLGFVGAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIG
                     IPASVESVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITG
                     RRLGVGQIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGVMASFYTQDPQVKHVVVILI
                     WLNALFMPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVG
                     VWMGMFADWAVRAVLFYWRMVTGRWLWKYPRPEPQKCEKKPVVSE"
     gene            3037..3112
                     /locus_tag="ACHKIR_002660"
     tRNA            3037..3112
                     /locus_tag="ACHKIR_002660"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3070..3072,aa:Asn,seq:gtt)
     gene            complement(3150..4100)
                     /gene="cbl"
                     /locus_tag="ACHKIR_002661"
     CDS             complement(3150..4100)
                     /gene="cbl"
                     /locus_tag="ACHKIR_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HFO9990377.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGTPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRIDDAFARKGLPA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEKNLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(4202..5119)
                     /gene="nac"
                     /locus_tag="ACHKIR_002662"
     CDS             complement(4202..5119)
                     /gene="nac"
                     /locus_tag="ACHKIR_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HFO9990378.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQSLSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            5446..5521
                     /locus_tag="ACHKIR_002663"
     tRNA            5446..5521
                     /locus_tag="ACHKIR_002663"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5479..5481,aa:Asn,seq:gtt)
     gene            complement(5576..6511)
                     /gene="ldtA"
                     /locus_tag="ACHKIR_002664"
     CDS             complement(5576..6511)
                     /gene="ldtA"
                     /locus_tag="ACHKIR_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFO9990379.1"
                     /translation="MMRRVNILCSFALLFASQNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKSGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQHRSGMPVQISSGSRQMF"
     gene            complement(6573..7652)
                     /gene="cobT"
                     /locus_tag="ACHKIR_002665"
     CDS             complement(6573..7652)
                     /gene="cobT"
                     /locus_tag="ACHKIR_002665"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HFO9990380.1"
                     /translation="MQILADLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGKLEALAI
                     QLAGMPGLNGIPHVGKKAILVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPISGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPRDGVDVLAKVGGFDLVGIAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMDMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(7664..8407)
                     /gene="cobS"
                     /locus_tag="ACHKIR_002666"
     CDS             complement(7664..8407)
                     /gene="cobS"
                     /locus_tag="ACHKIR_002666"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HFO9990381.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLVKILVLSELTLRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVVIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(8404..8949)
                     /gene="cobU"
                     /locus_tag="ACHKIR_002667"
     CDS             complement(8404..8949)
                     /gene="cobU"
                     /locus_tag="ACHKIR_002667"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HFO9990382.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRQGRPEHWRTVERWQHLDELIHADINPNEAVLLECVTTMVTNLLFDYGGDKDPDE
                     WNYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            complement(9083..9520)
                     /locus_tag="ACHKIR_002668"
     CDS             complement(9083..9520)
                     /locus_tag="ACHKIR_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HFO9990383.1"
                     /translation="MKTIKPESIALFCLLSGDVDLAKHLAEILPITCFVGEDWQQDFQ
                     PLNGGSVQSLSDAYSDYSVLLLIAAESEISVPEWLPHSEGTTVIVIAEHNARLLTGPA
                     GGAVALIHYLNEICHARETAVNDAFCRTLTRGSLAIPQCHLLS"
     regulatory      complement(9738..9913)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(10002..10916)
                     /gene="pocR"
                     /locus_tag="ACHKIR_002669"
     CDS             complement(10002..10916)
                     /gene="pocR"
                     /locus_tag="ACHKIR_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein PocR"
                     /protein_id="HFO9990384.1"
                     /translation="MISAATLNSELINKIAQDFAQATSLAVVVVNIHGDEISELFNFT
                     PFCQMMRQHPQLSTRCRMSDRCGGLEASKSDQPCIYRCHAGLTDFSIPLVIAGHLVGF
                     VLCGQVRLRNDDVDLVDILNVDDCWQADPELLNEFRKVPEMDYSRVMASADLLKLIVE
                     NCLKKQLNFVVIKENKSQSDSVRQTRAPNPHDNKMKKALRYIDAHLSDDLRLEDVASH
                     VYLSPYYFSKLFKKYHGIGFNAWVNQQRMASARELLCHSDWSIASIARNLGFSQTSYF
                     CKVFRQTYQVTPQVYRQQVSENAAMETV"
     gene            complement(11119..11928)
                     /gene="pduF"
                     /locus_tag="ACHKIR_002670"
     CDS             complement(11119..11928)
                     /gene="pduF"
                     /locus_tag="ACHKIR_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol diffusion facilitator PduF"
                     /protein_id="HFO9990385.1"
                     /translation="MNDSLKAQCVAEFLGTGLFLFFGIGCLCALKVTGASLGLWEICI
                     IWGLGISLAVYLTAGISGAHLNPAVTIALWLFASFPARKVLPFCVAQLAGAFGGAVLA
                     YSLYSSLFTDFESAHNIVRGSAESLQLASIFSTYPAAAINVWQAALVEVVITSMLMGL
                     IMALTDDGNGVPKGPLAPLLIGILVAVIGASTGPLTGFAMNPARDFGPKIFTWFAGWG
                     EIAMTGGRNIPYFIVPIIAPIIGACVGAAIYRYLIAKNLPVNSVTPKEISE"
     gene            12441..12725
                     /gene="pduA"
                     /locus_tag="ACHKIR_002671"
     CDS             12441..12725
                     /gene="pduA"
                     /locus_tag="ACHKIR_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduA"
                     /protein_id="HFO9990386.1"
                     /translation="MQQEALGMVETKGLTAAIEAADAMVKSANVLLVGYEKIGSGLVT
                     VIVRGDVGAVKAATDAGAAAARNVGEVKAVHVIPRPHTDVEKILPKGINP"
     gene            12722..13534
                     /gene="pduB"
                     /locus_tag="ACHKIR_002672"
     CDS             12722..13534
                     /gene="pduB"
                     /locus_tag="ACHKIR_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduB"
                     /protein_id="HFO9990387.1"
                     /translation="MSSNELVEQIMAQVIARVATPEHEVLPKTTHQIRETAMAEKSCT
                     LTEFVGTAIGDTVGLVIANVDSALLDAMKLEKRYRSIGILGARIGAGPHIMAADEAVK
                     ATNTEVISIELPRDTKGGAGHGSLILLGGNDVSDVKRGIEVALKELDRTFGDVYANEA
                     GHLELQYTARASYALEKAFGAPLGRACGVIVGAPAAVGVLMADTALKSANVDVVAYSS
                     PAQGTSYSNEVILIISGDSGAVRQAVISAREIGKTVLSTLGDTPKNDRPSYI"
     gene            13553..15217
                     /gene="pduC"
                     /locus_tag="ACHKIR_002673"
     CDS             13553..15217
                     /gene="pduC"
                     /locus_tag="ACHKIR_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol dehydratase large subunit PduC"
                     /protein_id="HFO9990388.1"
                     /translation="MRSKRFEALAKRPVNQDGFVKEWIEEGFIAMESPNDPKPSIKIV
                     NGVVTELDGKPANQFDLIDHFIARYGINLARAEEVIAMDSVKLANMLCDPNVKRSDIV
                     PLTTAMTPAKIVEVVSQMNVVEMMMAMQKMRARRTPSQQAHVTNIKDNPVQIAADAAE
                     GAWRGFDEQETTVAVARYAPFNAIALLVGSQVGRPGVLTQCSLEEATELKLGMLGHTC
                     YAETISVYGTEPVFTDGDDTPWSKGFLASSYASRGLKMRFTSGSGSEVQMGYAEGKSM
                     LYLEARCIYITKAAGVQGLQNGSVSCIGVPSAVPSGIRAVLAENLICSSLDLECASSN
                     DQTFTHSDMRRTARFLMQFLPGTDFISSGFSAVPNYDNMFAGSNEDAEDFDDYNVIQR
                     DLKVDGGLRPVREEDVIAIRNKAARALQAVFAGMGLPPITDEEVEAATYAHGSKDMPE
                     RNIVEDIKFAQEIINKNRNSLEVVKALAQGGFTDVAQDMLNMQKAKLTGDYLHTSAII
                     VDDGQVLSAVNDVNDYAGPATGYRLQGERWEEIKNIPGALDPNEID"
     gene            15227..15895
                     /locus_tag="ACHKIR_002674"
     CDS             15227..15895
                     /locus_tag="ACHKIR_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol/glycerol family dehydratase medium
                     subunit"
                     /protein_id="HFO9990389.1"
                     /translation="MEINEKLLRQIIEDVLAEMQTSDKPVSFRAPVSATASSAPDTGN
                     FLTEIGEAQQGTQQDEVIIAVGPAFGLAQTVNIIGIPHKNILREVIAGIEEEGIKARV
                     IRCFKSSDVAFVAVEGDRLSGSGIAIGIQSKGTTVIHQQGLPPLSNLELFPQAPLLTL
                     DTYRQIGKNAARYAKRESPQPVPTLNDQMARPKYQAKSAILHIKETKYVVTGKKPQEL
                     RVTF"
     gene            15910..16428
                     /gene="pduE"
                     /locus_tag="ACHKIR_002675"
     CDS             15910..16428
                     /gene="pduE"
                     /locus_tag="ACHKIR_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol dehydratase small subunit PduE"
                     /protein_id="HFO9990390.1"
                     /translation="MNTDAIESMVRDVLNRMNSLQDAAPVSAVPNASIQSAKVTDYPL
                     ANKHPEWVKTATNKTLDDFTLENVLSDNVTAQDMRITPETLRIQAAIARDAGRDRLAM
                     NFERAAELTSVPDDRILEIYNALRPYRSTKQELIAIADDLERRYQAKICAAFVREAAE
                     LYVERKKLKGDD"
     gene            16438..18270
                     /locus_tag="ACHKIR_002676"
     CDS             16438..18270
                     /locus_tag="ACHKIR_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diol dehydratase reactivase subunit alpha"
                     /protein_id="HFO9990391.1"
                     /translation="MRYIAGIDIGNSSTEVALATLSATGELSYVSSALAETTGIKGTL
                     RNVHGIQEALAQATKKVGINVSDISLIRINEATPVIGDVAMETITETIITESTMIGHN
                     PKTPGGVGLGVGLTITPQELLTRPADTPYILVVSSAFDFADIATMINASVRAGYQLTG
                     VILQRDDGVLVNNRLEIPLPIVDEVLYIDRIPLGMLAAIEVAVPGKVIETLSNPYGIA
                     TVFALNAEETKNIVPVARALIGNRSAVVVKTPSGDVKARSIPAGNIELLSAGRTTRVD
                     VAAGADAIMKAVGECPKLENVTGEPGSNIGGMLEHVRQTMAELTNKPSNEIFIQDLLA
                     IDTSVPVNVTGGLAGEFSLEQAVGIASMVKSDRLQMAMIASEIKQKLHVDVQVGGAEA
                     EAAIQGALTTPGTTRPLAILDLGAGSTDASIINQKGEIVATHLAGAGDMVTMIIAREL
                     GLNDRYLAEEIKKYPLAKVESLFHLRHEDGSVQFFPTPLSPHVFARVCVVKPDELVPI
                     LGDLTLEKVRAVRRSAKERVFVTNALRALRQVSPAGNIRDIPFVVLVGGSSLDFEVPQ
                     LVTDALAHYRLVAGRGNIRGSEGPRNAVATGLLIAWHKGAIHGK"
     gene            18260..18610
                     /locus_tag="ACHKIR_002677"
     CDS             18260..18610
                     /locus_tag="ACHKIR_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydratase reactivase beta/small
                     subunit family protein"
                     /protein_id="HFO9990392.1"
                     /translation="MESKERAPAIVVMEIGDCITTWDEVLLGIEEEGIPFRIQHIPSG
                     EVIDSAWLAARQSPLLVGIACDQEKLIVHYKNLPASAPLFTLMYQQDNHARRSIGTNA
                     ARLVKGIPFRELHS"
     gene            18630..18905
                     /gene="pduJ"
                     /locus_tag="ACHKIR_002678"
     CDS             18630..18905
                     /gene="pduJ"
                     /locus_tag="ACHKIR_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduJ"
                     /protein_id="HFO9990393.1"
                     /translation="MNNALGLVETKGLVGAIEAADAMVKSANVQLVGYEKIGSGLITV
                     MVRGDVGAVKAAVDAGSAAASAVGEVKSCHVIPRPHSDVEAILPKSA"
     gene            18929..19351
                     /locus_tag="ACHKIR_002679"
     CDS             18929..19351
                     /locus_tag="ACHKIR_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001607636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HFO9990394.1"
                     /translation="MKQSLGLLEVSGLALAISCADAMAKAASITLVGLEKTNGSGWTL
                     IKITGDVASVNAAIATGASFAGQRNGLVAHKVIARPGDGILPHTTEALTVTVETSETP
                     ATTVISEIISCNLCLDPACQRQKGEPRSLCLYANKRGE"
     gene            19354..19986
                     /locus_tag="ACHKIR_002680"
     CDS             19354..19986
                     /locus_tag="ACHKIR_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HFO9990395.1"
                     /translation="MDKQTLQETVSQILDEMRERPIPLGVSSRHIHLSTADYLRLFPE
                     QPISEKKPLMQPGQFAAEQVVTLEGPKGKLHNVRLLGPLRNASQVEISRTDARTLGIT
                     APLRMSGDLSGTPGIRLISPFAELEITSGVIVAQRHIHMSPLDALIYGVTHGDKVAVA
                     IEGDERRLIFENVTVRVSPDMRLEMHIDTDEANAAGADSPDVFATLVSVT"
     gene            19983..20474
                     /gene="pduM"
                     /locus_tag="ACHKIR_002681"
     CDS             19983..20474
                     /gene="pduM"
                     /locus_tag="ACHKIR_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcompartment protein PduM"
                     /protein_id="HFO9990396.1"
                     /translation="MNNMLMQRIVDEVVFRLKQRAGKTLVLTVFQLRDASVQESVHQY
                     ASLQIRYVDLPLLRQLAENETSDRAAIQIHEALAWGMHIQLSLQRHFLNAIELKTLAR
                     LPLSWCDEQGQPIYLHHDRLLSYVDIAQLSSGILVLQRKCCVTALAREAAITRNIQLI
                     RQE"
     gene            20478..20753
                     /locus_tag="ACHKIR_002682"
     CDS             20478..20753
                     /locus_tag="ACHKIR_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HFO9990397.1"
                     /translation="MHLARVTGAVVSTQKSPSLIGKKLLLVRQVSADGELPLTPLTGD
                     EVAVDSVGAGVGELVLLSSGSSARHVFSGPNEAIDLAVVGIVDTLSR"
     gene            20765..21765
                     /gene="pduO"
                     /locus_tag="ACHKIR_002683"
                     /pseudo
     CDS             20765..21765
                     /gene="pduO"
                     /locus_tag="ACHKIR_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460995.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-domain cob(I)yrinic acid a,c-diamide
                     adenosyltransferase PduO"
     gene            21762..23147
                     /gene="pduP"
                     /locus_tag="ACHKIR_002684"
     CDS             21762..23147
                     /gene="pduP"
                     /locus_tag="ACHKIR_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating propionaldehyde dehydrogenase
                     PduP"
                     /protein_id="HFO9990398.1"
                     /translation="MNNSELETLIRNILREQLVPATQRAQRNAIFQTVDEAVCAAHQA
                     FLRFQQCPLKTRSAIISAIREELMPRLTELAEESAKETGMGNKEDKYLKNKAALDNTP
                     GIEDLTTTALTGDGGMVLFEYSPFGVIGSVTPSTNPTETIINNSISMLAAGNSVYFSP
                     HPGAKNVSLKLITIIEDIAFRCSGIHNLIVTVSEPTFAATEQMMAHPKISVLAITGGP
                     GIVAMGMKSGKKVIGAGAGNPPCIVDETADIVKAAEDIINGASFDYNLPCIAEKSLIV
                     VVSVAEYLIQQMQSFGALLLNHEQTEKLRAICLPDGSANKKLVGKSPSALLEAAGLPL
                     PAKAPRLLIAVVDADDSWVTCEQLMPMLPIVKVNDFDSALALALKVEDGLHHTAIMHS
                     QNVSRLNLAARVMQTSIFVKNGPSWAGIGVGGEGFTTFTIATPTGEGTTSARTFARSR
                     RCVLTSGFSIR"
     gene            23158..24270
                     /locus_tag="ACHKIR_002685"
     CDS             23158..24270
                     /locus_tag="ACHKIR_002685"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0051144 - propanediol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-propanol dehydrogenase PduQ"
                     /protein_id="HFO9990399.1"
                     /translation="MKTFSLQTRLYSGTGSLNILNRFTNRHIWIICDVFLAHSPLIDT
                     LHQALPDSNRLSIFSEITPDPTIQTVVKGIAQMQTLRPDVVIGFGGGSALDAAKAIVW
                     FGRQCGIEIETCVAIPTTSGTGSEVTSACVISDPEKGIKYPLFDNALYPDIAILDPSL
                     VVSVPPAITANTGMDVLTHALEAYVSPRASDFTDALVEKAVQIVFQYLPTAVKKGDCL
                     ATRGKMHNASTLAGIAFSQAGLGLNHAIAHQLGGQFHLPHGLANALLLTAVIHFNAGD
                     PRAAKRYARLAKTCHLCPDNANDTASLNALIQHIEQLKTTCTLPTLTNALKEKKAEWS
                     IRIPDMVQAALADATLRTNPRAADASAIAELLEELL"
     gene            24267..25610
                     /locus_tag="ACHKIR_002686"
     CDS             24267..25610
                     /locus_tag="ACHKIR_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFO9990400.1"
                     /translation="MNVELTSCDDQTIRERVQAAGVVGAGGAGFPTHIKLQARVDTFL
                     VNAAECEPMLKVDQQLMVQQASRLLRGVHYAMKATGASSGIIALKEKYQRAINALTPL
                     LPPDIRLHILPDVYPAGDEVLTIWMATGRRVPPAALPVNVGVVVNNVQTVLNIARAVE
                     QQYPVTRRTLTINGAVAKPITVTVPIGMSLREVLALAGGATVDAPGFINGGPMMGNLI
                     TSLDTPVSKTTGGLLVLPNSHSLIQRRMQNDRSVLTVAKTVCEQCRLCTDLCPRHLVG
                     HELAPHLLVRAVNYQQLATPQLLLTALTCSECNVCASVACPVGISPMRINRLLKQELR
                     AQNLRYEGALNPADPMANYRLIPVKRLVTRLGLIPWYQDAPLSEQVPQPEKVTLLLRQ
                     HIGASAIACVQKGDRVVHGQCVGQIPHGTLGAPIHASIDGMVSDVTENAITLVRG"
     gene            25613..26167
                     /gene="pduT"
                     /locus_tag="ACHKIR_002687"
     CDS             25613..26167
                     /gene="pduT"
                     /locus_tag="ACHKIR_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduT"
                     /protein_id="HFO9990401.1"
                     /translation="MSKAVGILELSSIAKGMETGDAMLKSANVELLVSKTLCPGKFLL
                     MLGGDVGAVQQAINSGASLAGEMLVDSLVLPNIHPSLLSAISGLNHVEQHRAVGVVET
                     WSVAACIDAADCAVKAANVILVRVHMAFGIGGKCYMVVAGDISDVNNAVSVASERAGE
                     KGLLVYRSVIARPHEAMWRQMVEG"
     gene            26167..26517
                     /gene="pduU"
                     /locus_tag="ACHKIR_002688"
     CDS             26167..26517
                     /gene="pduU"
                     /locus_tag="ACHKIR_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000441103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduU"
                     /protein_id="HFO9990402.1"
                     /translation="MTTQHTTERMIQEYVPGKQVTLAHVIANPGKDLFKKLGLPDTVS
                     AIGILTITPSEASIIACDIATKSGAVEIGFLDRFTGAVVLTGDVSAIEYALSQVTRTL
                     GEMMRFTACPITRT"
     gene            26522..26965
                     /locus_tag="ACHKIR_002689"
     CDS             26522..26965
                     /locus_tag="ACHKIR_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HFO9990403.1"
                     /translation="MQRMMLIGPSQCGKTSLTQCLRGENIHYQKTQAIVWSPTTIDTP
                     GEYLENRSLYSALLVSACEADIIALVLNADAPWSPFSPGFTSSMNRPVIGVVTKADLA
                     SMEQISLVKCWLREAGAHNVLVTSAVNNNGVTELFALLHTEEGCR"
     gene            complement(27066..27395)
                     /gene="tmaR"
                     /locus_tag="ACHKIR_002690"
     CDS             complement(27066..27395)
                     /gene="tmaR"
                     /locus_tag="ACHKIR_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HFO9990404.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(27567..28625)
                     /gene="yeeA"
                     /locus_tag="ACHKIR_002691"
     CDS             complement(27567..28625)
                     /gene="yeeA"
                     /locus_tag="ACHKIR_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFO9990405.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSLVHALYEGNPQPVFANTEKLNDAVEELRQLLDNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSNLICRALRK"
     gene            complement(28823..29296)
                     /gene="sbmC"
                     /locus_tag="ACHKIR_002692"
     CDS             complement(28823..29296)
                     /gene="sbmC"
                     /locus_tag="ACHKIR_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HFO9990406.1"
                     /translation="MNYEIKQEEKRTVAGFHLVGPWEQTVKKGFEQLMMWIDSKNIVP
                     KEWVAVYYDNPDETPAEKLRCDTVVTVPNNFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            complement(29415..30581)
                     /gene="dacD"
                     /locus_tag="ACHKIR_002693"
     CDS             complement(29415..30581)
                     /gene="dacD"
                     /locus_tag="ACHKIR_002693"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HFO9990407.1"
                     /translation="MKRRLIIAASLFVFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVAMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIDLGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGGMFSRLSDYFHHKA"
     gene            30790..32217
                     /gene="sbcB"
                     /locus_tag="ACHKIR_002694"
     CDS             30790..32217
                     /gene="sbcB"
                     /locus_tag="ACHKIR_002694"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HFO9990408.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDSKFNVIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGY
                     NNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDS
                     DTLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNL
                     KILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQML
                     AQQYADDKEKVALLKALWQYAEEIV"
     gene            complement(32291..33649)
                     /gene="plaP"
                     /locus_tag="ACHKIR_002695"
     CDS             complement(32291..33649)
                     /gene="plaP"
                     /locus_tag="ACHKIR_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HFO9990409.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(33639..33701)
                     /gene="yoeI"
                     /locus_tag="ACHKIR_002696"
     CDS             complement(33639..33701)
                     /gene="yoeI"
                     /locus_tag="ACHKIR_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HFO9990410.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(33916..34845)
                     /gene="yeeY"
                     /locus_tag="ACHKIR_002697"
     CDS             complement(33916..34845)
                     /gene="yeeY"
                     /locus_tag="ACHKIR_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO9990411.1"
                     /translation="MKPLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(34891..35715)
                     /gene="yeeZ"
                     /locus_tag="ACHKIR_002698"
     CDS             complement(34891..35715)
                     /gene="yeeZ"
                     /locus_tag="ACHKIR_002698"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO9990412.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(35798..36052)
                     /gene="yoeB"
                     /locus_tag="ACHKIR_002699"
     CDS             complement(35798..36052)
                     /gene="yoeB"
                     /locus_tag="ACHKIR_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HFO9990413.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            complement(36049..36300)
                     /gene="yefM"
                     /locus_tag="ACHKIR_002700"
     CDS             complement(36049..36300)
                     /gene="yefM"
                     /locus_tag="ACHKIR_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HFO9990414.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
BASE COUNT         9746 a         9406 c         8576 g         8726 t
ORIGIN      
        1 gatttaccag taatgtaaat aaattttcga ggagatcatt ccagtgggac gtaaatgggc
       61 caatattgtt gctaaaaaaa cggctaaaga cggtgcaacg tctaaaattt atgcaaaatt
      121 cggtgtagaa atctatgctg ctgccaaaca aggtgaaccc gatccagaat taaacacatc
      181 tttaaaattc gttattgaac gtgcaaagca ggcacaagtt ccaaagcacg ttattgataa
      241 agcaattgat aaagccaaag gcggcggaga tgaaacgttc gtacagggac gttatgaagg
      301 ctttggtcct aatggctcaa tgattattgc tgagacattg acttcaaatg ttaaccgtac
      361 gattgctaac gttcgcacaa ttttcaataa aaaaggcggc aatatcggag cggcaggttc
      421 tgtcagctat atgtttgaca atacgggtgt gattgtattt aaaggaacag accctgacca
      481 tatttttgaa attttacttg aagctgaagt tgatgttcgt gatgtgactg aagaagaagg
      541 taacattgtt atttatactg aacctaccga ccttcataaa ggaatcgcgg ctctaaaagc
      601 agctggaatc actgagttct caacaacaga attagaaatg attgctcaat ctgaagttga
      661 gctttcccca gaagatttag aaatctttga agggcttgtt gatgcccttg aagatgacga
      721 cgatgtacaa aaagtttatc ataacgtcgc aaatctctaa tgatatttta aagaaatctg
      781 tctttatggc agatttcttt aatctcatat aattcttata aagaatataa tatccagctc
      841 gtcatattga ttataaaacc cacgctccca gagaaataat atttattaaa attccaggcc
      901 ttctttttct gattacagaa ggcaaagtgg caattacgta tagtttcccg ataaagacgt
      961 gacagcaaca tcccgcataa ggcatttttc tctttatctt tgtacggtac ttcacggaac
     1021 gaagtttttg accttgcgaa tcgtgatgtc tgttgggggg caatttgctc actgaagcgt
     1081 gagactcgat taaacgaacg aaacacagaa atcaaaaaac ccggtcactt tttttacaaa
     1141 gtgaccgggt aaaaataatt tttatttttt taactgtcct gagactcata gagatgtctc
     1201 aaaactaaaa tttggctcct ctgactggac tcgaaccagt gacatacgga ttaacagtcc
     1261 gccgttctac cgactgaact acagaggaat cgtgagaacg aggcgaatat tagcgatgcc
     1321 catccacaat gtcaaagcct gttttttaaa tttgaaatcg tttgctgaaa taatctgcat
     1381 tttgtcgttt attccgacac aactggcttt ttttcacact tttgcggctc gggtcgaggg
     1441 tatttccaca gccaacgtcc agtaaccatt cgccagtaaa acagcacggc ccgcacagcc
     1501 cagtcggcaa acattcccat ccagacacca accacacccc agccaagcat gattcccagc
     1561 acataaccga ctacaacccg gcaaccccac atgctcaaca tagaaaccca catggcgtaa
     1621 cgtgcatcgc gagccccctt aaatccagcg ggtagcaccc atgaggcgga ccaaataggc
     1681 ataaataaag catttagcca aatcagaatc acaacgacgt gtttaacctg tggatcctgg
     1741 gtgtaaaacg atgccataac cccggcaaag ggagccgtta gccaggcgat ggccgttaat
     1801 ccaagagtgg acagccagaa cacatgccgc aactgaatct ctgcttgcgc tatctgccct
     1861 actcccaacc ttcggcctgt aatgatcgta gaggcagaac cgagcgcact tccgggtaag
     1921 ttgataagag ccgcaattga gaacgcgata aaatttccgg caataacgct ggtccccatc
     1981 ccggcaacga acatttgagt taataaccga ccactggtaa ataacactga ttcgacactc
     2041 gctggaatac caatccccat gacttcccag ataatgctaa aattcagcgg tttaaaatag
     2101 ctctttaacg aaatccttag cgcaggatta aaaccaatcg ccagcaccca caaaattgca
     2161 actgcgccaa tataacgaga aatggttaaa cccaacccag ccccgacaaa tcccagtccc
     2221 ggccaggaga aaaggccgta aatcaatatg ccgctaataa taatattaag aatattcagg
     2281 ctaccgttaa tcagtagcgg tattttcgta ttccctgcac cacgaagtgc cccgctacca
     2341 ataagagtga tggcagctgc tggataactg agtacggtca gctccagata agtcaacgcc
     2401 agtgctttaa cttctgtcgt ggcatcaccc gcgacgaaat caataatttg ttcgccaaaa
     2461 tgatgaatta gcgttgccaa cagtacggca aacaacgtca tgatcaccaa tgactgccgc
     2521 gtcgccaccc tcgctcgtcg tcgatctcgc ttaccgagac taaatgctac aacgacagta
     2581 gtaccaagat cgatagctgc aaaaaaagcc ataacgacca tattgaagct gtccgccaac
     2641 cccacgccgg ccatcgcatc ttttcccagc cagctgacca gaaaagtgct caggaccccc
     2701 atcaacagga cacaggcgtt ctccatgaag ataggaacag caagcggggt tatctcgcgc
     2761 cagaacaaca ctttgtagct cttgcgttta gagtgccagc gagtgccgtg aacaacctgg
     2821 cgtaaagcag aggagatatt caaagccgac cttaattgca gaaagtgaaa ccacatttca
     2881 aatgatgagg gagaatcagc aaagctgcaa agattttcgc caacaaattg tctgcaaatg
     2941 caacaaactg ttgatagaaa cggcaaacag ttgggaaatt taaaaatcgg gtttgacaaa
     3001 atatttttcg ccgttaagat gtgcctcaac agcgattcct ctgtagttca gtcggtagaa
     3061 cggcggactg ttaatccgta tgtcactggt tcgagtccag tcagaggagc caaatttctg
     3121 aaaagcccgc ttttatagcg ggctttttgc tatatctgat aatcaatttc ctcttcactg
     3181 ttttccatca cctggcgctt gatatcctca actgacagtc ctgcattaca aagttccaga
     3241 aagcgccaga catagttacg ctgaagttgc cctcgcttca gtcccaacca gacagtatta
     3301 gcatcgaaaa gatgccgcgt atccaggcgg attaaattct tttcctcttg ttcgccgctg
     3361 gattgttcgg caactaatcc gatcccaagc ccaagagcaa cataggtttt aatgacatca
     3421 gaatcctgtg cacttaatac aatatctgcc ggcaaacctt tgcgggcaaa tgcgtcatca
     3481 atgcgtgagc gccccgtaat tccctgtcgg taagtaatta acggccactt cgctattgat
     3541 tccagcgtca atggtgtaat ttgcgttaag ggatgatcga gtggaacaag caaactatgg
     3601 tgccagcgaa accacgggaa ggcgacgagc tgcgggtcat tactcaaacg ctcgctggcg
     3661 ataccaatat cagcttcgcc attttgcaac aacgtcgcaa tttcctgtgg cgtaccctgg
     3721 attagctcga gccgaacctc cgggaaaagt tcgcgaaaag ctttaatgac ctctggcaag
     3781 ctataacgtg cctgagtatg cgtcgttgca atagtgagaa caccagacgt atcgttggta
     3841 aacaggtctg caagccgacg aacattactg gcttcattca gaatacgttc tgcaatgacc
     3901 agtaatgctt tgcccggttc agtcatgccc agcagtcgct tacctcgtcg aacaaatatt
     3961 tcgatgccaa gttcatcctc cagttcccga atatgacggc taacgcctga ctgtgaggta
     4021 aaaagcatat tcgcaacctc tgtcaggttg taatcctgac gtgcagcctc gcggattatc
     4081 tttagttgtt ggaaattcac ggtaaactcc gggcagttca gatatcccgt tattgttaaa
     4141 gtctaatgcc cggcataaca aataataaaa acccgcatct tattccatcc cgatataacg
     4201 cttagctcac caattgccac tgcctttttt ccatcactgg agaactaatc actgacatta
     4261 acaactcttt cactgcctgt gcctgtggtg acaagtttgc tctggcgggt aaatttaagg
     4321 acaaagagag actcattgaa ggagtggtaa tgcgtgacat ccacccattt actgcgccac
     4381 ataacgaacg cgcagccgat tcaggtaata ctgcaacgcc catgccgctg gcaatcgctg
     4441 cggtaagcgt ggcaatagac tcaatttcac caatgacttt tgccgtgagc cgccgtaggg
     4501 aaaaagcctc atcaacacga agtctaacag cgctgtaatc actggggaga aaaaggttca
     4561 tttgcgcaat agcattaaca tcaacgcttt gccccgggca atcttgagtt cctaccagga
     4621 aaagatcttc tttcagcaaa gcctgactgg ataccccagc cacaggggaa tgctcataaa
     4681 tcaccgccat atcgagttgg tgatttatca gtttttcgtt aagcactgca ccactatttt
     4741 catgaagata gataacgacc tccgggaatt cagcgcgaac cgcctgtaat aagggcatgg
     4801 tgatggatga cgcagcggtt cctggcgcaa agccaatcga gacttgcccc gataatgact
     4861 gaccaacgtt atgcaccgcc agttgggcct gctcacactg acgtaaaatg gcccgtgcat
     4921 gggtatagag aatttttccg gcgtctgttg gcgtaacgcc ccgttttgtg cgaatcaaaa
     4981 gctgttgatt taactcacct tccagtgtgg caacctgctg gctgagcgct ggttgtgcga
     5041 tatgcaatac ttcagcagcc tgggtcaggc taccaatatc tacaattttt acgaagtatt
     5101 tcaggcgtct gaagttcatg ttgcctccgg tttttaagaa tcggcccaag tgccgccatt
     5161 acttacaacc agaatgcaag atgcttgcca gttctatttt agcgttgatg cgcaagctaa
     5221 ccaactgtca aataagagat tatgatagat tcgtcattgg ccctttttat cagcagtcgc
     5281 attcacctgc cctataaaag gagggtatgc accacgatgg ttcattaccc aataagattg
     5341 aaagctcacc actttgttga aattggcagc aaacaaacaa aaaaatgcat ttcacccttt
     5401 gacatcacca tgcactgcca ttaatatgcg ccccgttcac acgattcctc tgtagttcag
     5461 tcggtagaac ggcggactgt taatccgtat gtcactggtt cgagtccagt cagaggagcc
     5521 aaattcaaaa aagcctgctt cgagcaggct ttttgctttc taattaccaa cgctcttaaa
     5581 acatctgtct tgaaccagaa ctaatttgca caggcattcc cgatcgatgt tgcaacgcag
     5641 catttgcgcg atttacatca acttcttgcc cgttgataaa cgcccgcaaa gatggggtta
     5701 ccggcaatgg cacttttcgg tcagactcat attctgcacg attgcgcgac agcggctcat
     5761 gaacttccag ccactttgaa ccatccggtt ccgttgtgta tttcactggc tgatcaataa
     5821 tttgtacacg agtgcctaca ggaacattat caaacagata tttgatatcg tcattgcgca
     5881 gacgaataca gccctgactt acccggagcc caataccaaa attggcattg gtaccgtgga
     5941 tggcatacaa cctgccaata taaatcgcgt acagccccat ggggttatcg ggccccgcag
     6001 gaacaaatgc gggcaaactc tcccctcgtt tcgcatattc gcgccgagtg ttcggcgttg
     6061 gcgtccaggt tggtgcttct tgtttacgtt caacggtagt cacccagtta cgcggggttt
     6121 ctcgcccagc ctggccgata ccaataggaa agacttccac tgtattactg tctggcggat
     6181 agtaataaag acgcatttca gcgacgttaa ccacaatccc tttacggaca gtggcgggca
     6241 aaatcagttg ctgcggaatg gtgagttgtg agccagactt cggcaaaaaa acatcagcgc
     6301 ccggattcgc ttccagcata ttacttaacc cttgcccgta ttgtgcggca aaagtctcca
     6361 gcggctgagt attgtgatca ggaacagtta cagtaagcga ctgccccact aaacggctac
     6421 cctctggagg taatggataa gttaccgcca ggctattttg gctggcaaaa agcagagcaa
     6481 atgagcaaag aatatttaca cgacgcatca tgtccctttc ctaagtcgcg aatactatcc
     6541 gataagtata gcttttatca gacttttcgt ttttagctgt tcaaatcaga agtcgtattc
     6601 cccggtagaa caatattact ggcagcaagt tcgcccatat tgttgtatat cgcacaagca
     6661 gcttcgatga tgggcatcgc cagagctgca ccactcccct cacctaaacg catatccata
     6721 ttgagataag gctccagccc caaatgcgac agcgctatac gcgcgccttt ttctgccgac
     6781 aagtgagaag gaatgagata cggtttgatt gcaggagaca tctggcaggc tgcgagcgca
     6841 gcagcataag aaagaaatcc atccagcaac acgggtaaac cgcaggaagc agcacctaac
     6901 atcactccag ctattccgac caaatcaaat ccacccactt ttgccaggac atcaacacca
     6961 tcccgaggat ttggttgatt caacgtaatc gcccgacgca caacatcaat tttattagcc
     7021 agtttatctg tcggcaggtt tgcgccaatc ccaaccactt cttcaggatc ccggccagtg
     7081 attgtgctga ctattgccgc tgccggtgtc gtgtttgcca tccccagttc acctacacca
     7141 aacagcgtga caccgttttt tgccagctcc cgcgtataac atatgacgtc caaaagcaac
     7201 ttttcagcct gacggcgact cattgccgga gctgaagcaa tattgccgct acctcgtgcg
     7261 acacgcatgt tgataagccc ggagataggc tcagcagtat caataccaac atcaattacg
     7321 tggacgttag cgcccgcttg tgctgccagc acacacacgc cggttgttcc gcgggtcata
     7381 ttttcagcct gtatggctgt cacttctttt ggagaaatag cgaccccttc ctcccagacg
     7441 ccgtgatcgg cacacataac cagtatcgct tttttgccca catgcggtat gccattcaac
     7501 cccggcattc ctgccagttg tatggcaagc gcctccagct ttcccaggct accaacaggt
     7561 ttgagtaacc cgtcaatatg ccgttgtgca cgcgacatag cggcaggatc gatggctggg
     7621 atcgtattca gtaaatcggc aagtatttgc atgtcacgtc tctttatagc agtgccagca
     7681 ggaaaaccag ttcaccaagc tctatggctg ctcccagcgt atcgcccgtt tgcccgccca
     7741 gcgtacgttt gagaagctga ccgaggatga aaatcaccac catcgtcacc accatagcag
     7801 ccacaccatg catacccggc aataatacgg cagcaaaaat agcggctaag ccgagtgtga
     7861 cgcaggtttg tcgcccatca attttgccaa tgaacacatt gccaagccct tcttcccgcg
     7921 cataacgatg acgatacatc aataatgcgg cagtcccgcg gctgaccgca catgctgccg
     7981 ccagcgatgc aaggatcggt tcgccacgca gagtcaactc gcttaacacc agaatcttta
     8041 ccagtaccac aaaaatcaat gccagaccgc cgtgggtgcc taaacggcta tcacgcatga
     8101 tttccaacat gcgatcgcgg ctacgtgcag aaaatacgcc atcacaggta tcagcgaggc
     8161 cgtccagatg gaatcccccg gtcatcagcg ccagcacaag tacgctaaac agtgccgcca
     8221 gtggtacgcc acaccatgcc tgcagcacca tgaagaccag cccgctaatc gcgccaagca
     8281 ataatccaat caaaggaaaa gtaataatac cgcgagaata atgctcgaaa tccagtccct
     8341 gggaccagcg acgcggtaca ggcagacgcg taataaatga gagcatcgcc caaaataatt
     8401 tactcatttg atttttactc caatacccga aaccaccagc catacttcat ttgccgcagc
     8461 ggccaactgc tgatttaccc gcccggcaat atcacgaaaa tgtcgtgcca gacgactctc
     8521 cggcacaatc cccattccca cttcgttagt cactaataca acctttgcgg ggcaacgttg
     8581 gcaggcagca atcaacgact gaatctcagc attaatcgcc tgttccatcg cctgataatt
     8641 ccattcatca gggtctttat cgccgccata atcaaacaac agattagtca ccattgtggt
     8701 aacgcattcc agcaacacag cctcattcgg gttaatgtct gcatgaatta attcatcaag
     8761 atgttgccaa cgctccactg tgcgccagtg ctccgggcgg ccttgccgat gatgttctat
     8821 ccgtgcagcc atctcatcat caaggatttg cgaggtggcg atatacagaa cctgtgaaga
     8881 gtccccaata agcgcctctg cgtggcgact cttcccgctc cgtgcgcccc ccgtcaccaa
     8941 aatcatcata ccgactcctg attcaattgt atgatggtat tcattgaaca gaacaatgga
     9001 tcccccaccg ttgctgaccg caaaacattt acgcgattaa tactgcgcgt aatataatta
     9061 aaaatccagc attctcaatc cattacgaaa gaagatggca ttgcggaata gccagacttc
     9121 ctcgtgttaa cgtccgacaa aatgcgtcat tgacagcggt ttctctggca tgacagattt
     9181 cattcagata atggatcaat gccacagccc caccagccgg accagtcagc aaacgggcat
     9241 tgtgttctgc aatcacaatt accgtcgttc cctcagaatg agggagccat tctggtacgg
     9301 atatctcgct ttcagctgct atcaatagca gcactgaata atcgctatac gcatctgata
     9361 atgattgcac agacccaccg ttaagaggct ggaaatcttg ctgccagtcc tcaccaacaa
     9421 aacaggttat tggcaatatt tcagccagat gtttcgccag gtccacatct ccagataaga
     9481 ggcaaaaaag agctatcgat tcgggtttta tcgttttcat gcctctcgcc ctccacttag
     9541 tttcctgtaa tgtcggtctg gttgatgagc catgaaacaa gattgccaca ccttaagtgc
     9601 agccctggtg cctgtcggca ctgcgatgaa agaactaaat gatgccgcca taccctgatc
     9661 ctttcagggt ggtaggggta taaagacgcg ccggtactct accggacgct tccgggcaac
     9721 ccacttccca ccgaagttgt tggtattgac tgacagactg gtcttctggc ttagcgtcat
     9781 cctcccccga ccttcccaat ctcgcgatca gtggtatttt cgggtttgtc agcattacag
     9841 cagcgggggc tgcgagggat tttcaccctc ttcccaggca caaatgtgcc acacctgtca
     9901 gcgtatgtta tgagctgatt tcacttttcc cataacacgt aaataacgtt aattattcat
     9961 tccagcgttt atttaaacct tccctttttt tagcatttac tctataccgt ttccatcgcc
    10021 gcattttcac tcacttgctg tcgatacacc tgcggcgtaa cctgataagt ctgacggaaa
    10081 actttgcaaa aataactggt ttgcgaaaaa cctaaattac gcgcaatgct ggcgatgctc
    10141 cagtcactat ggcacagtaa ctcacgagcg ctggccatcc gttgttggtt tacccacgca
    10201 ttaaaaccga ttccatggta tttcttgaat aacttactga aataataagg acttagataa
    10261 acgtgcgagg cgacatcttc cagacgcaaa tcgtctgata aatgcgcatc aatatagcgt
    10321 aaagcttttt tcattttatt gtcgtgggga ttaggcgcac gagtctggcg caccgaatct
    10381 gactgagatt tgttctcttt aataaccaca aaattgagtt gttttttgag gcaattttcc
    10441 acaatcaact tcaacaaatc agcagaggcc ataactcttg agtaatccat ctcaggaact
    10501 ttgcggaatt cattaagtaa ttcgggatct gcctgccagc aatcatcgac attcagaata
    10561 tcgaccaaat cgacatcatc attacgcaga cgaacttgcc cgcacaatac aaaaccaact
    10621 aaatgtccgg cgattaccag tggaatagag aaatcggtta atccggcatg acagcgataa
    10681 atacaaggct gatcggattt agaggcttcc agacccccac aacgatcgct catgcgacat
    10741 cgagtgctca gctgagggtg ttgccgcatc atctgacaaa atggagtgaa gttaaacagc
    10801 tcggaaattt catcgccgtg aatattgacc acgacaaccg caaggctggt cgcctgcgca
    10861 aaatcctgcg cgattttatt aatgagttct gaattaagtg tcgccgcaga aatcatgata
    10921 aaacctccca gttaatttat tgttatagca aaacaattgt tttacattta atcttcacat
    10981 catccttgat gatttactga cgagaagcac aaaggacaat aacaaaagcc tccgtgaagc
    11041 gggagacttt ttaataagtt cggaaagcag atcacaaaca gtaaaaaaat aaacgtttat
    11101 aactgccaga ttatttcgtt attcgctgat ttctttaggc gttacactat tgacaggtaa
    11161 attctttgca atgagatagc gataaatcgc cgcaccaaca catgccccaa tgatcggtgc
    11221 aataatgggt acaatgaaat aaggaatatt gcgtccacca gtcatcgcaa tttcccccca
    11281 acccgcaaac caggtgaaaa ttttaggtcc aaaatcacgc gctggattca tggcaaaacc
    11341 ggttaatggt ccggtagaag caccaataac agcaaccaga ataccaatga gtaaaggcgc
    11401 aagcggtcct ttgggtacgc cattaccgtc atcggtcaac gccataatca accccattaa
    11461 catggacgtt atgaccactt cgaccaacgc tgcttgccat acattaatcg ccgctgctgg
    11521 ataagtactg aagatactgg cgagttgtaa actttctgcg ctaccacgca ctatattgtg
    11581 agcagattca aaatcggtaa acaaactgct gtagagagaa tatgccagaa ccgcaccacc
    11641 aaaggcaccc gctaattgcg ccacacaaaa tggcagcact ttacgggctg ggaaagaggc
    11701 aaacagccac agagcaatgg taactgccgg gttgagatga gcgccggaaa tacctgccgt
    11761 aaggtaaaca gcaagcgaaa tccctaatcc ccaaatgata caaatttccc acagtcccag
    11821 acttgccccg gtaaccttca gggcgcacaa acaaccaatg ccaaaaaaga ggaacagtcc
    11881 ggtgcccaaa aactcggcaa cgcattgcgc cttgagtgaa tcattcatag tgacaccttc
    11941 tttacggagt ttttctttat cagccaacta tgcgctgatt gttgtgtata ccgagcgcaa
    12001 ctataaaaaa ttgctctgca gaaaatattg ccaacatgca tgacagactc gtgtaaaaat
    12061 cgtgctaaca taacaaatta ttgtcattgc cgaatcatgc aacacagcgt taatcagtgt
    12121 tgaaagtaat aatttccacc aaacttttac aataaaaaat tgccatcatg acataataaa
    12181 attgaaacaa atatcacagt tccttccttc gaaccaaact caacgaaata ttactcgcga
    12241 ttaaactcac tagccaacgc aattaattca cattgaaata tctacaaaac aaccaataaa
    12301 ttaacaacat atagttcata aaagcaattt tattatattt gttctctatt aatttttctc
    12361 attagccgat taagtcagcc attctttttc ctcgcttttt tttatagtca tatcgtcaaa
    12421 aaaatccagg cgaggtcttt atgcaacaag aagcgttagg aatggtagaa accaaaggct
    12481 taactgccgc catagaggcc gcagacgcca tggtgaagtc ggcaaatgtt ttgctggtcg
    12541 gctacgaaaa aataggttcc gggctggtga ccgtcatcgt tcgtggtgat gtcggggcgg
    12601 taaaagcggc aacagacgcg ggtgccgcag ctgcgcgtaa cgtcggcgaa gtaaaagccg
    12661 tacacgttat tccccgccca cataccgatg tcgaaaaaat cttaccaaag ggaattaacc
    12721 catgagcagc aatgaactgg tcgaacagat catggcgcag gtcatcgctc gcgtagcgac
    12781 gccagagcat gaggttctcc cgaaaaccac tcatcaaata cgagagacgg ctatggcaga
    12841 aaaaagctgc acgttaacag aatttgttgg caccgctatt ggcgacactg taggtctggt
    12901 cattgcgaac gttgatagcg ccctgttgga cgcgatgaaa cttgaaaaac gctatcgttc
    12961 cattggcatt ctgggggcgc gtattggcgc agggccacac attatggccg ctgatgaagc
    13021 ggtaaaagcc accaacactg aagttattag cattgaactt cctcgggata ctaaaggcgg
    13081 tgccggacac ggctcattga tcctcttagg tggcaacgat gtttccgacg tgaaacgcgg
    13141 tattgaagtt gccctgaaag aactcgatcg gacctttggt gatgtctatg ccaacgaagc
    13201 cgggcatctt gaactgcaat acaccgcccg cgccagctat gcactggaaa aagcgtttgg
    13261 cgctccactg ggacgggctt gcggcgttat tgtcggcgct ccggctgcgg tgggtgttct
    13321 gatggccgac accgcgctga agtccgccaa tgtggatgtc gtggcataca gctcccccgc
    13381 tcaaggcacc agctacagca acgaagtcat tctgattatc tcgggtgact ccggcgcagt
    13441 ccgccaggcg gtcatctccg cacgcgaaat cggtaaaacc gtgctttcga ctctcggcga
    13501 tacaccgaaa aacgatcgcc cttcctacat ctgaattctc tgaggcagat ttatgagatc
    13561 gaaaagattt gaagcactgg caaaacgccc ggtcaaccag gacggtttcg ttaaagagtg
    13621 gattgaagaa ggcttcattg cgatggaaag cccgaacgat cctaaaccgt caatcaaaat
    13681 tgttaacggc gtcgttaccg aactggatgg aaaacccgcc aatcagtttg acctgatcga
    13741 ccactttatc gcccgctacg gcatcaatct cgcgcgcgca gaagaagtca tcgcgatgga
    13801 ttcggtaaaa cttgccaata tgttgtgcga tccaaatgtc aaacgtagcg atatcgttcc
    13861 ccttaccaca gcaatgacgc ctgcaaaaat cgtcgaagtg gtttcgcaga tgaacgtggt
    13921 agagatgatg atggcgatgc agaaaatgcg cgcccgtcga acaccatcac aacaggctca
    13981 cgtcaccaac atcaaagata acccggtaca aattgccgcc gacgcagctg aaggcgcatg
    14041 gcgcgggttt gatgaacagg aaaccaccgt tgccgttgct cgctatgcac cgttcaacgc
    14101 catcgccctg ctggtcggct cacaggtggg acgtccgggc gtcttgaccc agtgttcact
    14161 ggaagaagcc accgaactga aactcggtat gttaggccac acctgttacg ccgaaacaat
    14221 ttctgtctat ggtacagaac ctgttttcac cgatggcgat gataccccgt ggtcaaaagg
    14281 gtttctcgcg tcatcctatg cgtctcgcgg cttaaaaatg cgctttactt ccggctccgg
    14341 ctctgaagta cagatgggtt acgcggaagg caaatcaatg ctttatctcg aggctcgatg
    14401 catttacatc accaaagccg caggcgttca gggtctgcag aatggttcgg taagctgtat
    14461 cggtgttcca tcagctgtgc catcaggcat ccgcgcagta ttagcggaaa acttaatttg
    14521 ctcatcgctg gatctggaat gcgcctccag taatgaccag acctttaccc actcggatat
    14581 gcgccgtacc gcacgtttcc tgatgcaatt tctgccaggt accgacttta tctcttccgg
    14641 tttttccgct gtgccgaact acgacaacat gtttgctggt tcaaacgaag atgctgaaga
    14701 cttcgatgat tacaacgtca ttcagcgtga cctgaaggtt gatggcggcc tgcgaccagt
    14761 acgcgaagaa gatgttatcg ctatccgcaa taaagccgct cgcgctttac aggctgtctt
    14821 tgccggtatg ggactaccgc ctattaccga tgaagaagtc gaagcggcaa cctatgccca
    14881 cggttcaaaa gatatgcctg agcgcaatat tgtagaagac atcaagttcg cccaggagat
    14941 tatcaataaa aaccggaaca gcctggaagt ggtgaaagcc ctggcgcaag gcggttttac
    15001 cgatgtcgcc caggacatgc tcaacatgca aaaagcaaaa ctgaccggcg attatctcca
    15061 tacctcagcc atcatcgtcg atgacggaca agtgctctct gcggtcaatg acgtcaatga
    15121 ttacgccgga ccggctacag gttaccgcct gcaaggggaa cgctgggaag agatcaaaaa
    15181 tattcccggt gcacttgatc ccaacgaaat tgactaaggg tgagaaatgg aaattaacga
    15241 aaaattactg cgtcagatta tcgaagacgt gctggcagaa atgcaaacca gcgacaagcc
    15301 cgtttcattt cgcgcacctg tctctgcaac tgcctcttcc gcgccagata ccggaaattt
    15361 tctgaccgaa attggcgaag cccaacaagg cactcagcaa gatgaagtca ttattgctgt
    15421 aggcccggcg ttcggcctgg cgcaaaccgt caatatcatc ggtattccgc ataaaaacat
    15481 tttgcgcgaa gtgattgccg gtatcgaaga agaaggcatt aaagcccgtg tgattcgctg
    15541 ctttaaatcg tccgatgtcg cattcgtagc agtcgaaggc gatcgtctta gtggttcagg
    15601 tattgctatc ggtattcagt cgaaaggcac aacggttatc caccagcagg gacttcctcc
    15661 cttgtctaac ctggaacttt tcccacaagc gcctttgttg acgctggata cttatcgtca
    15721 gattggcaaa aacgccgcgc gttatgcaaa gcgtgagtct ccacaaccgg tgccgaccct
    15781 caacgatcaa atggcgcggc cgaaatacca ggccaaatct gccattttgc atattaaaga
    15841 gacgaagtac gtggtcaccg gcaaaaaacc gcaagaactg cgcgtgacgt tttaacaaag
    15901 gaaaccttca tgaataccga tgcaattgag tcgatggtca gagacgtgtt gaaccgcatg
    15961 aacagtttac aggatgcagc tcctgtttct gcggtcccca acgcgtcaat acagagcgcc
    16021 aaagtgacgg attacccgct ggcaaataaa catccggaat gggttaaaac ggcgaccaat
    16081 aaaacgctcg acgatttcac tctggaaaat gtgctaagcg ataacgtcac cgcccaggat
    16141 atgcgtataa cccctgaaac attgcgtatt caggccgcaa ttgccagaga tgctggtcgc
    16201 gatcgcctgg cgatgaattt tgaacgcgcc gctgagctta cctcagtacc ggatgatcgc
    16261 atcctcgaaa tctacaatgc gttacgccct tatcgttcga ctaagcaaga gcttattgcc
    16321 attgctgacg atcttgaacg gcgttatcag gccaaaattt gtgctgcatt tgtccgtgaa
    16381 gctgcggaac tttatgtcga gcgcaaaaaa ctgaaaggcg acgattaagg acgaactatg
    16441 cgatatatag caggcattga tatcggcaac tcatcaacag aggttgcgct ggcaacgctc
    16501 agtgcaactg gagagttgtc gtacgtgagt agcgccctgg cggaaaccac cggaattaaa
    16561 ggtacgttgc gtaacgtcca tggtattcag gaggcgctcg cacaggcaac gaaaaaagtc
    16621 ggcatcaatg ttagcgatat ttcgcttatt cgtatcaacg aagcaactcc ggtcattggt
    16681 gatgtggcga tggaaaccat cactgaaacc atcatcaccg aatcaaccat gatcggtcac
    16741 aacccgaaaa ctccgggcgg agttgggctg ggcgtagggc tcaccatcac tccgcaagag
    16801 ttattaacac gcccggcgga cacaccttac atcctggtgg tgtcatcggc atttgatttt
    16861 gccgatatcg caacgatgat aaacgcctcc gtacgagccg gatatcagct gactggcgtt
    16921 attctgcaac gcgacgatgg cgtactggtc aataaccgcc tggaaattcc tctccctatt
    16981 gtggatgaag tcctctacat cgatcgtatt ccgctcggca tgctggctgc aattgaagtt
    17041 gccgttcccg ggaaagttat cgaaaccctg tctaacccgt atgggattgc caccgttttc
    17101 gccctcaatg ctgaagagac taaaaatatt gttcctgtcg cgcgggcgct aatcggcaac
    17161 cgttcagcag tggtagtgaa aacgccttct ggcgacgtta aagcccgttc gatacccgcc
    17221 ggaaatatcg aactgctttc tgccgggcgc acaacgcggg ttgatgtagc tgctggtgcc
    17281 gatgccatta tgaaagcggt cggtgagtgc ccaaaactgg aaaacgtgac gggagaacct
    17341 ggctccaaca tcggcggaat gctggaacat gttcgccaga ccatggctga actgaccaac
    17401 aaacccagca acgaaatttt tattcaggat ttgctggcga tagatacctc tgtccccgtc
    17461 aacgtaaccg gtggtctggc aggtgaattt tcactggaac aggctgtggg tattgcctct
    17521 atggtgaaat ccgaccgtct gcaaatggcg atgatcgcca gtgaaattaa gcaaaaactg
    17581 catgttgatg ttcaggtcgg aggcgcagaa gcagaagccg ccattcaggg ggccttgact
    17641 acccctggaa ccacacgtcc actggcaatt ctggacttag gtgcaggctc taccgatgcc
    17701 tccatcatca atcagaaagg ggaaatagtt gccactcacc ttgcgggagc gggcgacatg
    17761 gtaacgatga ttatcgctcg tgagctgggc ttaaatgatc gttatctcgc agaagaaatc
    17821 aaaaagtacc cgttggcaaa agtagaaagc ctgttccact tacgccatga agatggcagc
    17881 gttcagttct tccctactcc gctgtcccct catgtatttg cccgcgtttg tgtggtgaaa
    17941 cctgacgaac tggtgcctat tctcggtgat ttgacgctgg aaaaagtacg tgctgtccgt
    18001 cgtagtgcga aagagcgtgt cttcgtaact aatgccctgc gcgcgctacg tcaggtaagc
    18061 cctgccggaa atattcgcga cattccgttc gtcgttctgg tgggtggttc ttcgctggat
    18121 ttcgaagtac cgcaattggt caccgacgcg ctggcacatt atcgactggt cgccgggcgg
    18181 gggaatatcc gtggtagcga aggtccgcgc aatgcggtag ctactgggct cctcatcgcc
    18241 tggcacaagg gggcgattca tggaaagtaa agagcgtgca ccagccatcg ttgtcatgga
    18301 gatcggtgac tgtatcacta catgggatga agtgcttctt ggcattgaag aggaaggtat
    18361 tccctttcgc attcaacaca ttccgtcagg tgaagttatc gatagcgcct ggctggcggc
    18421 tcgtcaatct cccctgttgg ttggcattgc ctgcgatcag gaaaaactga ttgtgcatta
    18481 caaaaactta ccggcatcag cgccactgtt tacgctgatg tatcaacaag ataaccatgc
    18541 caggcgcagc atcggcacta acgctgcccg cctggtaaaa gggattccct tccgggaact
    18601 ccactcataa tccacaggag aaacgcagta tgaacaacgc actaggactg gttgaaacaa
    18661 aagggttagt tggcgcaatt gaagctgcag atgccatggt taaatccgcc aacgtgcagc
    18721 tggttggcta tgaaaagatt ggttcgggtc tgattacggt gatggtccgt ggcgatgttg
    18781 gcgcagtaaa agccgccgtt gatgcaggaa gcgccgccgc aagcgccgtt ggcgaagtga
    18841 aatcctgcca tgtcatcccg cgcccacaca gtgatgttga ggctatttta cctaaatcag
    18901 cataacccca ttcgctaagg agcacagcgt gaagcaatca ctgggattac ttgaagtcag
    18961 tggtctggca ctcgccatca gttgcgcaga tgctatggca aaagccgcgt ccatcacgct
    19021 ggttggcctt gagaagacca atggctcagg ctggacgctc atcaaaatca ccggtgacgt
    19081 cgcctcggtt aatgcggcga tcgcgacggg agccagtttc gccgggcagc gaaatggcct
    19141 tgtcgcacac aaagtaattg cccgcccggg agacggcatt ctgccccaca caactgaagc
    19201 attgactgtg acggttgaaa caagcgaaac accagcaaca accgtcatct cagagattat
    19261 cagttgtaat ttgtgtcttg acccggcatg tcagcggcaa aaaggtgagc cccgctcact
    19321 ttgcctgtac gccaataagc gaggggagta gcagtggata aacaaaccct gcaggaaaca
    19381 gtgagccaga ttctggatga gatgcgcgag cgccctatcc cactgggcgt ttccagtcgc
    19441 catattcatc tttcaacagc ggattatttg cgtctttttc ctgaacaacc gattagcgag
    19501 aaaaagccgt taatgcaacc gggccaattc gctgctgaac aggttgttac gctggaggga
    19561 ccaaaaggca aattgcacaa cgttcgcttg ttggggccgc tacgcaatgc aagccaggtg
    19621 gaaatttccc ggacggacgc cagaacgtta gggatcaccg ccccgctacg catgtcaggc
    19681 gatctcagcg gcactccggg gattcgtttg ataagcccat ttgcggaact ggagatcaca
    19741 tctggcgtca ttgtcgcgca acggcatatt catatgtctc cgctggatgc cctgatttat
    19801 ggtgttacgc acggcgataa agtggcggtt gccattgaag gtgatgagcg acgactgata
    19861 ttcgaaaacg tcactgttcg cgtctctccg gatatgcgtc ttgaaatgca tattgatacc
    19921 gatgaagcca acgccgcagg ggctgatagc cctgacgttt ttgccacact ggtgtcagtg
    19981 acatgaataa catgctgatg caacgtattg ttgacgaagt ggtttttcgc cttaaacaac
    20041 gtgcgggaaa aacactcgtg ctgactgttt ttcaactgcg agacgccagc gttcaagaga
    20101 gcgttcatca gtatgcgtca ttacaaatcc gatatgtcga tctgccgctg ctgcgacaac
    20161 ttgcggagaa cgaaacgtca gatcgagcag caatccagat acacgaagcg ttagcctggg
    20221 gaatgcacat tcaattgtcg ttgcagcgtc atttcctgaa cgccattgaa ttgaagactc
    20281 tcgcccgtct accgctaagc tggtgcgacg aacaagggca accgatctac ctacatcatg
    20341 atcgactttt aagttatgtc gatatcgcac agttaagctc gggaatattg gtgttacagc
    20401 ggaaatgttg tgtgaccgca ctggctcgtg aagcggcaat tacccgaaat attcagttaa
    20461 tcaggcagga gtaacctatg catctggctc gagtcactgg cgcggtagta tccacgcaaa
    20521 aatcaccttc tctgatcggg aaaaaactgc tgctggttcg ccaggtcagt gctgacggcg
    20581 aactgcctct cacgccatta accggcgacg aagttgccgt tgattccgtc ggcgcaggcg
    20641 ttggtgagtt ggtactcctc agcagtggtt ccagcgccag acatgtgttt tccggcccta
    20701 acgaagccat cgacctggca gtggtcggca ttgtcgatac gctttcgcgc taaggacctg
    20761 aaagatggca atttataccc gaacaggtga ttcaggaagt acgtctttat ttaccggaca
    20821 acgcgtcagc aaaactcatc tacgtgtcga aacctatggc acgctggatg aactgaacgc
    20881 gacactcagt ctgtgttatt gcgccaccgc catcgaaagc catcgcatct tactggaagc
    20941 cattcaacaa cagatatttt ggtttagcgc cgaactcgcc agtgaaagcg aacagccgtc
    21001 agcgcagcag cgttacatcg gtacggaaga aattgccgcg ctggaaaacg cgatcgatag
    21061 cgcaatgaac gccgtccccc cggttcactg cttcattctg cctggtcgat gtgaagccgc
    21121 atgcatttcg cccgcacggt ggctcgacgg gctgaaagac gtctggttga actgacaaca
    21181 gaaaccaccg tccggaatgt tttgctgcac tatatcaacc gactttccga ttgcctgtat
    21241 gccctcgccc gcgtggaaga caacgttgct catcaaaacc tgatgattca ggaaatcaca
    21301 aaacgttatc acgaagccaa ccacacacca gcattgaagg aacgcacaat gccgctcact
    21361 tttcaggatc ttcaccagct cattcgtagt gcggcaatgc gcgcagatga actgcatatt
    21421 cccgttgtca tcagcattgt cgatgccaac ggaacagaaa gtgttacctg gcggatgcct
    21481 gatgccctgt tggtcagcag tgaactggca ccgaaaaaag cctggaccgc cgtggcgatg
    21541 aaaacggcaa cccacaagct tgctgatacc gtgcaacccg gcgcgccgct ttatgggctc
    21601 gaaagccata tgcaaggcaa agtcgtcacc tttggtggcg gcttccccct ctggcgtgac
    21661 ggaaaattgc ttggcgggct tggcatcagc ggcggtagcg ttgaacaaga catggatatt
    21721 gcccaaagcg caatggcggc aattaacgtg ggagtaaacc aatgaataac tctgaattag
    21781 aaacactgat ccgcaatatt ctgcgtgagc agcttgtccc cgctacgcaa cgagcgcaaa
    21841 gaaacgctat tttccagact gttgatgaag cggtctgcgc agcacaccag gcctttttac
    21901 gctttcaaca gtgcccgctt aaaacccgta gcgccattat cagcgccatt cgcgaagaat
    21961 taatgccccg ccttaccgaa ctggcagaag aaagcgccaa agaaacgggg atgggaaata
    22021 aagaagataa gtacctaaaa aacaaagcgg cacttgataa cacgcctgga attgaagatt
    22081 taaccaccac agcactgacc ggtgatggag gcatggtttt gtttgaatac tctccgtttg
    22141 gggtgattgg ctcggtcacg ccaagcacca accccacgga aaccatcatt aacaacagca
    22201 tcagcatgct ggcagcaggt aatagcgtct acttcagtcc acacccggga gccaaaaacg
    22261 tttcgttaaa actgattacc ataatcgaag acatcgcgtt tcgctgtagt ggcattcaca
    22321 acctgatcgt taccgtatct gaaccaacct ttgccgcaac cgaacaaatg atggcgcacc
    22381 ctaaaatcag cgttctggcg attaccggtg gcccggggat cgtcgccatg ggaatgaaaa
    22441 gtggcaagaa agtgattggt gccggagcgg gtaacccacc ctgcattgtc gatgaaacgg
    22501 cggatattgt gaaagccgcc gaggacatca tcaacggcgc ttcatttgat tacaacctgc
    22561 cgtgtatcgc tgaaaaaagc ctgattgtcg tggtatcagt ggcagagtat ttaattcagc
    22621 agatgcaaag ctttggagca cttcttctta accatgaaca aacggagaaa ttgcgtgcaa
    22681 tttgcctgcc agacggtagc gccaacaaaa aactggttgg gaaaagccca tcagccttgc
    22741 tggaagcagc gggcctccct ttgcccgcaa aagcgccgcg cctactgatt gctgttgttg
    22801 acgccgatga tagttgggtt acgtgtgaac aactgatgcc aatgctgccc attgtgaagg
    22861 tcaacgattt tgacagcgct ctcgccctgg cattgaaagt ggaagacggc ttgcaccaca
    22921 cggcaattat gcactcgcag aacgtctcac ggctcaacct tgccgcgcgc gttatgcaaa
    22981 cctcaatctt tgtcaaaaac ggcccctcct gggcgggtat tggcgtcggc ggcgaaggct
    23041 ttacgacctt taccattgca acaccaaccg gagagggtac aacgtcagca cggacttttg
    23101 cccgctcacg acgctgcgtg ttaaccagcg gcttttccat tcgctagaga aacggaaatg
    23161 aaaacttttt cactgcagac gcgtttatac agcggaacgg gaagtctcaa catactgaac
    23221 cgtttcacga ataggcacat ctggattatt tgcgacgtct ttctggcaca ctcaccactc
    23281 attgacacgt tgcatcaggc gctgcccgac agtaatcgtc tcagtatttt tagcgaaata
    23341 acgcccgacc caactatcca gaccgtggtg aaaggcattg cgcaaatgca gactttacgc
    23401 ccggatgtgg ttatcggttt tggcggcggt tctgccctgg acgccgcaaa agcgattgtc
    23461 tggttcggtc gccagtgtgg cattgaaatt gaaacctgcg tagccattcc taccaccagc
    23521 ggtaccggtt ccgaagtgac cagtgcttgt gtcatcagcg atcctgagaa agggattaaa
    23581 tatccccttt tcgataatgc actttatccg gatatcgcga tccttgatcc atctttggtc
    23641 gtcagcgtgc caccggcgat aacggccaat accgggatgg atgttttaac ccatgcgctt
    23701 gaagcttatg tttctccccg cgccagcgac ttcacggatg cactggtcga aaaagccgtg
    23761 caaattgttt ttcaatacct gcccactgca gtgaagaaag gcgattgtct tgccacccgt
    23821 ggcaaaatgc ataatgcatc gacgcttgca ggaatagcct ttagccaggc cggactcggt
    23881 ttaaaccatg ccattgctca tcaactcggc ggacagtttc atctgccgca cggcctggcg
    23941 aatgcgctcc tgctcacggc ggttatccac tttaatgctg gcgatccgcg cgcagccaaa
    24001 cgctacgccc ggctggcgaa aacgtgccat ttatgcccgg acaacgccaa tgacaccgcc
    24061 agcctgaatg cacttattca acatattgag cagcttaaaa caacctgtac gctgccgact
    24121 cttaccaatg cgctgaaaga aaaaaaagca gaatggtcaa tacgcatacc agatatggtt
    24181 caggcggcac tggcggatgc aacgttgcgt accaacccgc gtgcggctga tgcctccgct
    24241 attgcagaac tgctcgagga gttgttatga acgtcgagtt aacctcctgc gacgaccaga
    24301 ccatccgcga gcgagtacag gcagcaggtg tcgtgggtgc tggcggcgca ggttttccaa
    24361 cccatattaa gctacaagcg cgtgttgaca cttttctggt gaatgcggcg gaatgcgaac
    24421 caatgttgaa agtggatcaa cagttaatgg tgcagcaagc cagccgcctg cttcgcggtg
    24481 ttcactatgc gatgaaagcc accggggcca gtagcggaat catcgcgctg aaagagaaat
    24541 atcaacgggc aataaacgca ctcacccccc tattaccacc ggatatccgc ctgcatattt
    24601 tgcccgacgt ttaccccgca ggagacgaag tgctgaccat ctggatggca actggtcgcc
    24661 gcgttccacc cgctgcatta cccgttaacg tcggtgtggt ggtcaataat gttcaaaccg
    24721 tacttaatat tgccagagcc gtcgagcaac agtatccagt gacgcgccgc acactcacta
    24781 ttaatggtgc agtggcgaaa ccaataacgg taaccgtccc catcggcatg tctttacgtg
    24841 aagtcctggc gctggcgggc ggcgcaacag tagatgctcc tggttttatc aatggcgggc
    24901 cgatgatggg aaatttgatc acatcgctcg acacccccgt cagcaaaacc actggcggct
    24961 tgctggtttt acccaactct cattcactga tccaacgtcg aatgcagaat gaccgcagcg
    25021 tacttaccgt cgcgaaaact gtttgtgagc aatgccgctt gtgtaccgat ctctgcccac
    25081 gtcatttagt cggtcacgaa ctggcacccc atttattagt acgtgcggtt aactaccaac
    25141 agctcgctac gccacaactg ttactgacgg cgctcacctg ttcggagtgc aacgtctgcg
    25201 ccagcgtcgc ctgtccggta ggaatttcac caatgcgcat aaaccgcctg cttaagcagg
    25261 aattacgcgc acaaaatctc cgctacgaag gtgccttaaa tcctgctgat cccatggcaa
    25321 attatcgact gattcccgta aaacggctgg taaccagact gggcctaatc ccgtggtatc
    25381 aggatgcgcc attgtctgaa caggtgcctc agcccgaaaa agtgacgtta ttgttacgcc
    25441 agcacatcgg tgccagcgca attgcgtgtg tacaaaaagg cgatcgcgtc gttcatgggc
    25501 aatgtgtagg tcaaattcca cacggcacat taggtgcccc cattcatgcc agtattgatg
    25561 gcatggtcag cgatgtgaca gaaaacgcga ttacgcttgt gagaggttaa cgatgtcgaa
    25621 agcggtaggt attttagaac ttagcagtat cgcgaaaggg atggaaaccg gcgacgccat
    25681 gctgaaaagc gcaaacgttg agttactggt cagcaaaacg ctatgccccg gaaaatttct
    25741 gctgatgctc ggtggcgatg tgggtgcggt gcaacaggcc atcaacagcg gtgcttccct
    25801 tgctggtgaa atgctggtcg acagtcttgt cctgccaaat attcatccca gtctcctttc
    25861 tgcaatcagc ggactgaacc atgttgaaca gcaccgggct gtcggcgtag tcgaaacctg
    25921 gagtgtcgcc gcctgcattg acgctgccga ttgtgctgtc aaagctgcga atgtcattct
    25981 cgttcgcgta catatggctt ttggcattgg tggtaaatgc tacatggtgg ttgctggcga
    26041 tatttcagat gttaataacg cggtctctgt cgccagcgag cgtgcggggg aaaaaggttt
    26101 gttggtctat cggtcagtga ttgcccgtcc acatgaagcc atgtggcgtc agatggtgga
    26161 gggataatga caactcagca cacaactgaa cggatgattc aggaatatgt ccctggtaag
    26221 caggtcacgc tggcacatgt catcgccaat cctgggaagg atctgtttaa gaaattgggt
    26281 ttaccagaca ccgtttctgc tattggcatt ctgacgatta cccccagcga agcatctatc
    26341 atcgcatgtg atatcgccac aaaatcaggc gctgtagaga tcggttttct tgatcgtttt
    26401 accggtgccg tagtgctgac tggtgatgtt tctgctattg aatatgcact tagccaggtt
    26461 acccgtacgc tgggtgaaat gatgcgcttt accgcctgcc ccattacgcg gacctgacgt
    26521 tatgcaacgt atgatgctta tcggccccag ccagtgcgga aaaacgtcac tcacgcaatg
    26581 tctgcgtggc gaaaatatcc attatcagaa aacgcaggca atcgtctggt ccccgaccac
    26641 cattgataca ccaggcgaat acctcgaaaa ccgcagcctc tacagtgcat tgttggtcag
    26701 tgcctgcgaa gcagacatta tcgcgctggt acttaatgcg gacgcaccgt ggtcaccttt
    26761 ttcgccaggc tttacctcgt caatgaatcg cccggtcatt ggagtagtaa ccaaagcaga
    26821 tttagccagc atggaacaaa tatctttggt gaaatgttgg ttacgggaag ctggagcgca
    26881 caacgtgtta gtaaccagtg cggttaataa caatggagtt acagaacttt ttgccttgct
    26941 gcatacagaa gaaggctgcc gctaatttcg ataggttctc agatgagaat gatgacggtg
    27001 atacacagat aagaatcaat gagcaatgag ggttgccggg caaccctcat tgaataaaac
    27061 aggaattact tcgcttcgcc gtttttcatt tcgcccatag ctttcagctt tttggagata
    27121 tcgcggcgtt ctttggagag ctcggcattt ttgatgatgt aatcgtcaac gcgatcttca
    27181 tagtcacctt tcatgctggc gatgatgccc tggattgctt caacgctcat ccctggcttg
    27241 atgtaatcgc tcaggttgtc cagcagcagg acgcgcttct ggttgtcacg gatctttttc
    27301 tcaacgtcct gaatttcacg ttgcagtttg ttcttacgac ggaacagacg aacaaattcc
    27361 agtacgtcct ggaatgaagg cttggtagtt tccattttta tacccctgat aatgtgagag
    27421 tcggattcgt ttaagcaacc gctattcgtt agggccaaca ttactcatag ctgccgcaga
    27481 tgacaatgct tttatccctt ttcactatca taccctttat ccttgctgaa tcgaagcagc
    27541 agcaagatga ttctgaagtt caggaattat ttgcgcaagg cccggcaaat cagattcgat
    27601 agcaactcaa gctgatgtgc cgtttccatg ttcagccaca cataaccata gattggtgtt
    27661 tccacaacct tcaggtcatg gtggttatcg agcaactgac gcagctcttc cacagcatcg
    27721 ttcaattttt ccgtattggc aaaaaccggc tgcggattac cttcgtacag cgcatgaaca
    27781 aggctcagca gtatttgctg catcatgtgc tgggtatcac gaagtttttg cgcgttcaat
    27841 aacacgaaat ggctggggcg cgtggcccag tatgcattga tttgcaactc cagcatacaa
    27901 accagattgc gattaatggt ctggatacct tcatatatcg attttggaat acgggtttct
    27961 ttgctggcgg gcgcaatcag cccacgcatt ttcacggcat cggtcaggag tttttgtaga
    28021 tggctttcca gacgtgggcg ttcgagtaag ttcggtgaga atgcagattg atagacccga
    28081 ttatactcgg tcagactttt cgccagttga atgcgccagt ggatgaacgc ccgttgtggc
    28141 cagataccgg taaacaacat tgccagtaaa gagccgagga tcacatcgcc gcttcgccat
    28201 aacgccgtat caatttcacc tgtcggggaa cccacaacaa ttgccagcgt caccccaatc
    28261 aataaacctt gatatggttt cttgcccagc gccagccaac cgcaaaggaa catggccgcc
    28321 gcgcaccaga ctaacatcag cggtaacgag attaactcca gttgcagagc gataagacct
    28381 aaaatcgacc ccaacaccgt accgccaata cgttcgaagg cacgagggac aacgttcccc
    28441 cagaacgaga ttggccccat aatcaccacc atggtgacca gcggccaggt gctttccggg
    28501 atagtaaaca ggcggataat gagaaaagtg agcaggaatg ccagcgcgac ccgagtacca
    28561 tgcacaatgc ggtaatggcg gtatacccgg atttcaaacg ggcttaatga cttatcggca
    28621 cgcacgcaga aactccgtaa tgaagattaa aagcgctcat aactcatctg tcggccagaa
    28681 ggcgcgcctc tgaattatcc gacggacaaa ccgggaacag tgtaatttcc cggcaggagc
    28741 gaaaaaattg aaaggcgcat cttactcttt tcgctttcaa aaaaagagtg gtcttcgcgt
    28801 caacacaccg ccctgagatg aattagtgat gttttggctg caccgcaaca tacatttcga
    28861 tatcccagta cccatcttcc gcgccattgt tcaagtaaac ctcgaagcag ggctttggta
    28921 acatttcata agcactgtcc tgcaagaggc tattaaagaa ctgataccag ggtttagcaa
    28981 aatcatcacc gactacacga gccaccgcca ccgcatactg accacctgaa atttctgtca
    29041 gaatgacgcc ctcactgttt tcggggagcg taaagttatt cggtaccgtc acgacggtgt
    29101 cgcagcgtaa tttttcggcg ggtgtttcat ctggattgtc gtaatagaca gcaacccact
    29161 ccttcggcac aatatttttg ctatctatcc acatcatcaa ctgctcaaaa cctttcttta
    29221 ccgtctgctc ccacgggcca acaagatgga aacctgcaac ggtacgtttc tcttcctgct
    29281 taatctcgta gttcatgacg cctccattaa cactgttttt atatacagta tagttgcaat
    29341 attaaaacca caaggaatga gtgttgatta tgcgagcaga ctcgcactcc tgccagtctg
    29401 ctgcaaaaga aaggtcaggc cttatggtgg aaataatcac tcaggcggga aaacatgccg
    29461 ccttccccga cagattccag ggtaaccagc ggccagtgcg ccacctgttt atcacggtcg
    29521 taaagttcaa tttcccctac ccgctgatgg gcgctaattg gcgcggtgag ctctttacca
    29581 tcaagggtat atttggcttt gatatgtgga atttcggctt tcggtagcac catccagaac
    29641 tcttgttccg ttcccaggtc gatattttct ttatcgccat accagatgcg ttccgttccg
    29701 acctttttcc cacggtgcaa aatttgcacc gtagtaaagt tttgttgccc ccaacgcagt
    29761 aattttcttg cctcttcctc acgacctttt gcactgtcag cccccattac cactgcaatg
    29821 agacgacgct gcccatctac agccgaagca atgagattaa acccggcacc agaagtatga
    29881 cccgttttca ggccgtcaac attcatggtt ttatcccaca acaacccgtt acggttttgc
    29941 tgggtgatac cgttccaggt gaggcttttc tcactgtaca tatgataaaa ctcgggctcg
    30001 ccgtggatga tagcgcgaga aagcacagct aaatcataag ccgagctatg ctggccaggt
    30061 gcatccagac catgcactgt ttcaaaatgc gtatccttga gatgcagctt ctcggcatag
    30121 ttgttcatca ttgcaacaaa ctgccgttgc ccaccggcaa tatagtcagc cagtgcaaca
    30181 caagcatcat ttccggaatc cacaattaaa ccacggctta aatcacgtac cgatacgcga
    30241 tcgccctctt tcaaaaacat cagtgaagaa ccgacaaaca ctggattatc tttcgcccac
    30301 gcatcgcgcc ctacggtgac aatatcgtct ggcgtaattc gatgactatc gatagcgcga
    30361 tccacgacat aacccgtcat cagctttgtc aggctggcgg gattgcgctg ttgatgctca
    30421 ttacccgcgg tgaggatctg tccggtggtg taatccatca atacccagga cccggcatga
    30481 atctctggag gctgaggtga aaaaggaatg ttttccgccg caaaaccaga cgataagtta
    30541 aaaacgaaca aagaagcagc aataataaga cggcgtttca acagcaaacc ctcaggagtt
    30601 tcaaatagct gttcttttta cggaaatact tatgaactgg ctggaataaa gtgcaagaaa
    30661 atgtgactaa cctctcattt ttatctgaca tgatctgttg ccactcgctg ccaaattgtg
    30721 gcgctaaagc tgattagcac ggtgatattt gatactctgg cagacagcag agataacgga
    30781 tttaacctaa tgatgaatga cggtaagcaa caatctacct ttttgtttca cgattacgaa
    30841 acctttggca cgcaccccgc gttagatcgc cctgcacagt tcgcagccat tcgcaccgat
    30901 agcaaattca atgtcatcgg cgaacccgaa gtcttttact gcaagcccgc ggatgactat
    30961 ttaccccagc ctggagcagt attaattacc ggtattaccc cgcaggaggc acgggcgaaa
    31021 ggagaaaacg aagccgcgtt tgccgcccgt attcactcgc tttttaccgt accgaagacc
    31081 tgtattctgg gctacaacaa tgtgcgtttc gacgacgaag tcacacgcaa cgttttttat
    31141 cgtaatttct acgatcctta cgcctggagc tggcagcatg ataactcgcg ctgggattta
    31201 ctggatgtta tgcgtgcctg ttatgccctg cgcccggaag gaataaactg gcctgaaaat
    31261 gatgacggtc taccgagctt tcgccttgag catttaacca aagcgaatgg tattgaacat
    31321 agcaacgccc acgatgcgat ggctgatgtg tacgccacta ttgcgatggc gaaactggta
    31381 aaaacgcgtc agccacgcct gtttgattat ctctttaccc atcgtaataa acacaaactg
    31441 atggcgttga ttgatgttcc gcagatgaaa cccttggttc acgtttccgg aatgtttggg
    31501 gcatggcgcg gcaataccag ctgggtggca ccgctggcgt ggcatcctga aaatcgcaat
    31561 gccgtaatta tggtggattt ggcaggagac atttcgccat tactggagct ggatagcgac
    31621 acattgcgcg agcgtttata taccgcaaaa gccgatcttg gcgataacgc cgccgttccg
    31681 gttaagctgg tgcatatcaa taaatgtccg gtactggccc aggcgaatac gctacgcccg
    31741 gaagatgccg accgactggg aattaatcgt cagcattgcc tcgataacct aaaaattctg
    31801 cgtgaaaatc cgcaagtgcg tgaaaaagtg gtggcgatat tcgcggaagc cgaaccgttt
    31861 acgccttcag ataacgtgga tgcacagctt tataatggct ttttcagtga cgccgatcgt
    31921 gcagcaatga aaattgtgct ggaaaccgag ccgcgtaatt taccggcact ggatatcact
    31981 tttgttgata aacgaattga aaaactgttg ttcaattatc gagcgcgcaa cttcccgggg
    32041 acgctggatt atgccgagca gcaacgctgg ctggagcacc gccgccaggt cttcacgcca
    32101 gagtttttgc agggctacgc tgatgaattg cagatgctgg cacaacagta tgctgatgac
    32161 aaagagaaag tggcgctgtt aaaagcgctt tggcagtacg cggaagagat cgtctgaaac
    32221 aaaaaagccg gagcgacaaa ctccggcttt ttgagattta ccggatgatt atgtcatccg
    32281 gtttgtaaga ttacgctacg tcttcgtact gcggaaccgg gttacggaaa ctcttggtta
    32341 cgcaagccag gtaaatcaga ccgatagcag cccagatcag acccagaacc attgagcttt
    32401 cttccaggtt aacccacagc gcaccaacgg tcagcgcacc acacatcggc aggaacagat
    32461 actggaagtg atctttcagc gtcttgttac gcttctcacg gatccagaac tgcgagatga
    32521 ccgacaggtt aacgaaggtg aacgccacca acgcaccaaa gttaatcagc gccgtagcca
    32581 ttaccaggtc gaagttgatt gccagcaggg caatcgcgcc aaccaggatg atgttcatcg
    32641 ccggagtacg ccatttcggg tgtacataac cgaagaagct tttcgggaac acgccgtcac
    32701 gacccattac gtacatcaga cgcgctacgc ctgcgtgcgc cgccataccg gacgccagta
    32761 cggtaatggt ggagaagatc agcgcgccaa cctggaacgc tttacccgca acgtacagca
    32821 tgatttcagg ctgtgacgca tccggatctt taaagcgaga gatatccggg aagtacagct
    32881 gcaggaagta agttgcaaag atgaagatca tgccgccgat cagcgcggtc aggaaaatcg
    32941 cacgcgggat cacgcgctct gcatctttgg tttcttccga caggttgctg atgccgtcaa
    33001 aaccggtaaa ggagaagcac aggattgtcg ccccggtaat catcgggata acatgcgcat
    33061 cgccagacca gaacggacga gtgctcgcca gcgtaccagc gccttcacct tcaaatacgc
    33121 cataaacaac catgcccaga atcaccgcga tcagcactac ctgcaacacg acgattacag
    33181 tgttgaagtt cgctacggat ttcagactac gcaggttaaa ggcggtcata aaggccacca
    33241 gcgccaccac aaacatccac gatggaatgg aaggcaccag agcttcaaaa tagattttcg
    33301 ccagcagaat gttgatcatc ggcgcgaaca gatagtcgag cagagaagac caacccacca
    33361 taaagccgac agtcgggcta atggatttct gggcgtaagt gtatgcagag ccagcagaag
    33421 gatagcggcg aaccagcttc ccgtagctca gagccgtaaa caggatcgca atcaatgcga
    33481 acgcataggc tgtcggcaca tgaccatccg tgaggcctga aacgatacca aaagtatcaa
    33541 acagcgtcat cggctgcata taggctagac ccatcattac aaccggaact aacgtaagcg
    33601 ttttacgcaa ttccacgcga gaggtgtttg gagtaacgtt atgcgacatg gtcattctcc
    33661 tttacggtga taaccgtcgc gtaagcaaaa aattgcccca tttttttgga ttcctcagcg
    33721 acaacaactg tcgattttta gtaaatatct atccggtacg aagcccggcc tcttggtatg
    33781 aatgattggt ttgaagcaaa aaataaccga cgctaattaa acgtcggttt ttagtcattt
    33841 tttgacagcg gcgcattgtg cctaaatggg ggggaaatga caagagaatg agaggcttgt
    33901 cataataatt tttctttaaa tggctgattt tccgtcatca gattttgcat aaacaccggc
    33961 gatcgcacta tttgctaaaa tttcgtcccg ccaccaacca ctggcaagcc ctgcggtctg
    34021 ttcattccag ccaatccaga cgggttcaaa gagagtttgg gcgaccactt tcttctcgat
    34081 taacgcgcca ctatcgagaa aacgttgcgc cagataccgt ggtaaatagc cgcagccaag
    34141 gccgctaatt tgcagctcca gcttggtttt aaaatcgaaa acggtaatgg cttcttgttc
    34201 gtcgagaagt tgtgaggccg tagaagcgcc tgcctgcgca gtgtccccca ccacaatcgc
    34261 ccgatatcgt ttaatgatac gacggtttaa cggttcttct tcctgggcta acggatgatg
    34321 aggcgcgacc gcaaagactt gttcaagatc gcccaaccgc gagaaaccaa actcactgga
    34381 agatggtggc tcatgcatcg cgccaacaat aatatccgct cgcccctgag tcagtgcatc
    34441 ccaggaacca ccgagtacgc cattgataaa cttcaggcgc gtcacgctat gatgttgata
    34501 aaaagcctca atgagaggcg caagcagaga aaaaggaaac gtgtcgtcca cgccaatcac
    34561 cagctcattt tcccagcctt catgcagttt gatagcctgc ttttccagtt ctcgcacggt
    34621 atgcagaact tcccgccctt tctctaataa catttttccg gtgcgggtga atttagcgcg
    34681 gtgaccgcta cgatcaagca attgaatatt aaggtcgctt tccagcttgt gaacggtata
    34741 actcagagcg gacggcgtct tgtagagttt agccgacgcc gccgcaaaac tgccttcttt
    34801 ttctaaggca tcgaggatca tcaacacatc cagcagtggt ttcatacacg cccccttgca
    34861 gtgtatgcgg cgtcctgctg gcgtgatcgt ttactccagc ggcattacca gcggatcggg
    34921 atactggtat tcaaatccca gttcattaca aatccgactg ccatcaataa tcttaccttt
    34981 gccgctgtcc agactatttc tgaactgcgg aggttccagc cccagtaaac gggccatctg
    35041 cggatagaaa acattacgcg cagggtgagc tggcgcacat atattataga tgtgtccgcc
    35101 tttaggtgcc tgtaacaaca gagtgatagc accaataaca tcttctaaat ggactaaatt
    35161 aacaccatgt tcaccatcag gggcggtttt tccggcaaag aagcgtccgg gatgacgtcc
    35221 cggtccgacc agccccgcaa gacgcagaat atcgaccgaa gtaccgggta aattgtgcag
    35281 ccagtcttcg agttcttcta atactcgtcc actgttggtc accggattac gcggggtggt
    35341 ttctttcacc gtgccttgcg cgtcgccata gacagatgtt gagctggtaa aaataattcg
    35401 gggaatacga tgggccagcg cgctatccac taactcttgt accgcttgta aatagaactc
    35461 atcgccgggg ccgctacgac gtgccggaag cgtaatgacc agcgcgtcgg catccatcag
    35521 ggcatccaga tcgtcagaat cgcaaactaa ctcaggctcc atgcgaagca gatagctatc
    35581 aatgccactc attcgggccg cttcgacacc atcttgtgtg gttttactcc cggtgacttg
    35641 ccagcctcgc gctgaaagtg acatcgccag cggcatgcct aaccacccta acccgacaat
    35701 tgcgaccttt ttcatccgtt atctcctgat acaccttaga tctataaggc tacgctagcg
    35761 tatcaaaact gacaattcat tctatgaatg aatctgttca ataatgataa cgacatgctg
    35821 caatgagtag tgaatcgtcg gtaaccgcgt ataccagacg gtgctcctct gtaatgcgtc
    35881 gggaccagaa acctgacaaa ttatgtttca ggggttctgg tttcccctta ccttcaaatg
    35941 gtgttctgcg ggtatctttg ataagttcat tgatcttttt aacaattcgc ttatctgttt
    36001 cctgccagta cagataatca tcccatgatt cctcagacca gattagtttc actcaatgat
    36061 gtccttttcc gttcctttgc cagatttcag gctatcgatt gagtccatca atctccgggc
    36121 gttagcgggg gagcgcagta gataagccgt ctcctccagg gagttgtatt cttcgagtga
    36181 catcagaaca caagcctctc cattctgacg agtaataagg atcggggcat gatcttcaac
    36241 ggctttcatc attgttgccg acaaattctg acgcgcttcg ctgtagctaa ttgtacgcat
    36301 gtcaatctcc tcttttgtac agttcattgt acaatgatgg gcgttaatta actatttatt
    36361 aattagtttg tagatcaagg tattgtcagt gagacgaaaa tccaggcttc gctatttttg
    36421 gtgccgtcag cttagaggac agtcctctta gccc
//
LOCUS       DAZGQL010000050        36418 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.050, whole genome shotgun sequence.
ACCESSION   DAZGQL010000050 DAZGQL010000000
VERSION     DAZGQL010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36418
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.050"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            327..1700
                     /gene="aroP"
                     /locus_tag="ACHKIR_002701"
     CDS             327..1700
                     /gene="aroP"
                     /locus_tag="ACHKIR_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HFO9990415.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTCFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(1743..2597)
                     /gene="ampE"
                     /locus_tag="ACHKIR_002702"
     CDS             complement(1743..2597)
                     /gene="ampE"
                     /locus_tag="ACHKIR_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HFO9990416.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQIDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVLVVIAL
                     LTIYGALV"
     gene            complement(2594..3145)
                     /gene="ampD"
                     /locus_tag="ACHKIR_002703"
     CDS             complement(2594..3145)
                     /gene="ampD"
                     /locus_tag="ACHKIR_002703"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HFO9990417.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLVAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            3233..4126
                     /gene="nadC"
                     /locus_tag="ACHKIR_002704"
     CDS             3233..4126
                     /gene="nadC"
                     /locus_tag="ACHKIR_002704"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HFO9990418.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANE
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            4329..4769
                     /gene="ppdD"
                     /locus_tag="ACHKIR_002705"
     CDS             4329..4769
                     /gene="ppdD"
                     /locus_tag="ACHKIR_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HFO9990419.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN"
     gene            4779..6164
                     /gene="gspE"
                     /locus_tag="ACHKIR_002706"
     CDS             4779..6164
                     /gene="gspE"
                     /locus_tag="ACHKIR_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HFO9990420.1"
                     /translation="MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAIHEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVLPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNAISFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQTLNTADINICSVENPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAINAAQTGHLVLSTLHTNSTCETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDNVWPSPLPRWQAPGCVHCYHGF
                     YGRTALFEVLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGE"
     gene            6154..7356
                     /gene="hofC"
                     /locus_tag="ACHKIR_002707"
     CDS             6154..7356
                     /gene="hofC"
                     /locus_tag="ACHKIR_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HFO9990421.1"
                     /translation="MASKQLWRWRGITGDGNAQDGMLWAESRALLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGVAFSNALFPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQKAQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPVLTQGIMTLADFSGEWGW
                     LLVLFGFLLAIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGQPIWLALKNAGEFSPLCLQLVRTE
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(7391..8434)
                     /gene="guaC"
                     /locus_tag="ACHKIR_002708"
     CDS             complement(7391..8434)
                     /gene="guaC"
                     /locus_tag="ACHKIR_002708"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFO9990422.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSFEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVLEQENRIFNNL"
     gene            complement(8590..8634)
                     /locus_tag="ACHKIR_002709"
     CDS             complement(8590..8634)
                     /locus_tag="ACHKIR_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9990423.1"
                     /translation="MIIKTLKMSARKRN"
     gene            8659..9279
                     /gene="coaE"
                     /locus_tag="ACHKIR_002710"
     CDS             8659..9279
                     /gene="coaE"
                     /locus_tag="ACHKIR_002710"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFO9990424.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     PREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            9279..10022
                     /gene="zapD"
                     /locus_tag="ACHKIR_002711"
     CDS             9279..10022
                     /gene="zapD"
                     /locus_tag="ACHKIR_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HFO9990425.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPVVDYAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDSENGQVPERLDFELACC"
     gene            10032..10229
                     /gene="yacG"
                     /locus_tag="ACHKIR_002712"
     CDS             10032..10229
                     /gene="yacG"
                     /locus_tag="ACHKIR_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HFO9990426.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(10431..10829)
                     /gene="mutT"
                     /locus_tag="ACHKIR_002713"
     CDS             complement(10431..10829)
                     /gene="mutT"
                     /locus_tag="ACHKIR_002713"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HFO9990427.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHLSLFEILEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVLAKLKRVYVG"
     gene            complement(10889..13594)
                     /gene="secA"
                     /locus_tag="ACHKIR_002714"
     CDS             complement(10889..13594)
                     /gene="secA"
                     /locus_tag="ACHKIR_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFO9990428.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLAAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(13656..14168)
                     /gene="secM"
                     /locus_tag="ACHKIR_002715"
     CDS             complement(13656..14168)
                     /gene="secM"
                     /locus_tag="ACHKIR_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secA translation cis-regulator SecM"
                     /protein_id="HFO9990429.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLS"
     gene            complement(14399..15316)
                     /gene="lpxC"
                     /locus_tag="ACHKIR_002716"
     CDS             complement(14399..15316)
                     /gene="lpxC"
                     /locus_tag="ACHKIR_002716"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HFO9990430.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(15417..16568)
                     /gene="ftsZ"
                     /locus_tag="ACHKIR_002717"
     CDS             complement(15417..16568)
                     /gene="ftsZ"
                     /locus_tag="ACHKIR_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFO9990431.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(16629..17891)
                     /gene="ftsA"
                     /locus_tag="ACHKIR_002718"
     CDS             complement(16629..17891)
                     /gene="ftsA"
                     /locus_tag="ACHKIR_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFO9990432.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(17888..18718)
                     /gene="ftsQ"
                     /locus_tag="ACHKIR_002719"
     CDS             complement(17888..18718)
                     /gene="ftsQ"
                     /locus_tag="ACHKIR_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HFO9990433.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(18720..19640)
                     /gene="ddlB"
                     /locus_tag="ACHKIR_002720"
     CDS             complement(18720..19640)
                     /gene="ddlB"
                     /locus_tag="ACHKIR_002720"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFO9990434.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDANPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(19633..21108)
                     /gene="murC"
                     /locus_tag="ACHKIR_002721"
     CDS             complement(19633..21108)
                     /gene="murC"
                     /locus_tag="ACHKIR_002721"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFO9990435.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(21162..22229)
                     /gene="murG"
                     /locus_tag="ACHKIR_002722"
     CDS             complement(21162..22229)
                     /gene="murG"
                     /locus_tag="ACHKIR_002722"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFO9990436.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(22226..23470)
                     /gene="ftsW"
                     /locus_tag="ACHKIR_002723"
     CDS             complement(22226..23470)
                     /gene="ftsW"
                     /locus_tag="ACHKIR_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HFO9990437.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(23470..24786)
                     /gene="murD"
                     /locus_tag="ACHKIR_002724"
     CDS             complement(23470..24786)
                     /gene="murD"
                     /locus_tag="ACHKIR_002724"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFO9990438.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGGLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAVTGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKICVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWVNDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(24789..25871)
                     /gene="mraY"
                     /locus_tag="ACHKIR_002725"
     CDS             complement(24789..25871)
                     /gene="mraY"
                     /locus_tag="ACHKIR_002725"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFO9990439.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLVGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(25865..27223)
                     /gene="murF"
                     /locus_tag="ACHKIR_002726"
     CDS             complement(25865..27223)
                     /gene="murF"
                     /locus_tag="ACHKIR_002726"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFO9990440.1"
                     /translation="MISVTLSQLTDILNGELKGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVNRPLDIDLPQVIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHIQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFSDKTALIARLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(27220..28707)
                     /gene="murE"
                     /locus_tag="ACHKIR_002727"
     CDS             complement(27220..28707)
                     /gene="murE"
                     /locus_tag="ACHKIR_002727"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HFO9990441.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(28694..30460)
                     /gene="ftsI"
                     /locus_tag="ACHKIR_002728"
     CDS             complement(28694..30460)
                     /gene="ftsI"
                     /locus_tag="ACHKIR_002728"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HFO9990442.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMAEYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTVPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(30476..30841)
                     /gene="ftsL"
                     /locus_tag="ACHKIR_002729"
     CDS             complement(30476..30841)
                     /gene="ftsL"
                     /locus_tag="ACHKIR_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFO9990443.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(30838..31779)
                     /gene="rsmH"
                     /locus_tag="ACHKIR_002730"
     CDS             complement(30838..31779)
                     /gene="rsmH"
                     /locus_tag="ACHKIR_002730"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFO9990444.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(31781..32239)
                     /gene="mraZ"
                     /locus_tag="ACHKIR_002731"
     CDS             complement(31781..32239)
                     /gene="mraZ"
                     /locus_tag="ACHKIR_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFO9990445.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
     gene            complement(32841..33845)
                     /gene="cra"
                     /locus_tag="ACHKIR_002732"
     CDS             complement(32841..33845)
                     /gene="cra"
                     /locus_tag="ACHKIR_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009750 - response to fructose [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HFO9990446.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(33927..34013)
                     /locus_tag="ACHKIR_002733"
     CDS             complement(33927..34013)
                     /locus_tag="ACHKIR_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HFO9990447.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(34025..34516)
                     /gene="ilvN"
                     /locus_tag="ACHKIR_002734"
     CDS             complement(34025..34516)
                     /gene="ilvN"
                     /locus_tag="ACHKIR_002734"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFO9990448.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(34519..36243)
                     /gene="ilvI"
                     /locus_tag="ACHKIR_002735"
     CDS             complement(34519..36243)
                     /gene="ilvI"
                     /locus_tag="ACHKIR_002735"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HFO9990449.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTSGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     TAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESVHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTNGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPQELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            36362..36406
                     /locus_tag="ACHKIR_002736"
     CDS             36362..36406
                     /locus_tag="ACHKIR_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9990450.1"
                     /translation="MKRNDKILHYRGLN"
BASE COUNT         8730 a        10193 c         8939 g         8556 t
ORIGIN      
        1 gattattgtg ctggtggcta aggcatagtt ttcagcaggc agacaaatcc ggcatatagc
       61 tagttaaaac agtatgttat cagacttaaa gtatctatta aatgtgatga aaaacttaca
      121 attgcttttt ttttatccaa aaaatcaacg catctacata aagcaggtgc attcccgggc
      181 acataccatt atcttgtgat agttaaaagt agcattatca taattcagtt acatgaaacg
      241 taaagaaatc aatacaacaa acggaattgc aaacttacac acgcatcact gcgtagatca
      301 aaaaaacaat caccgcacga ggtttcatga tggaaggtca acagcacggc gagcagctaa
      361 agcgcggtct taaaaaccgc catattcagc ttatcgcgct gggtggcgcg atagggaccg
      421 ggctattcct gggtagcgcc tccgtaatac agtccgcagg gccagggatt atcctgggtt
      481 acgccattgc tggttttatc gcctttctga tcatgcgtca gctaggtgaa atggtggtcg
      541 aagaacctgt cgcaggctcc tttagccact ttgcttataa atactggggc agttttgccg
      601 gtttcgcctc tggctggaac tactgggtac tgtacgtttt agttgccatg gctgagctga
      661 ctgccgtggg taaatacatt cagttctggt atccggaaat cccaacctgg gtttctgccg
      721 ccgtgttctt tgtggtgatt aacgccatca acctgaccaa cgttaaagtg tttggcgaga
      781 tggagttctg gtttgccatt atcaaagtta tcgcggtggt agcgatgatc atcttcggcg
      841 gctggctgct attcagcggc aacggcggcc cgcaggcgag cgttagcaac ctgtgggatc
      901 agggtggttt cctgccgcac ggcttcaccg ggctggtgat gatgatggcg attatcatgt
      961 tctctttcgg cggtctggaa ctggtgggga tcaccgcagc agaagctgat aatccggagc
     1021 aaagtatccc gaaagcaact aaccaggtta tctaccgcat cctgattttc tatattggtt
     1081 cgttagccgt tctgctctcg ctgatgccat ggacccgcgt tactgctgat accagtccgt
     1141 ttgtactgat cttccacgaa ttgggcgata cctttgtggc taatgcgctg aacatcgtgg
     1201 tactgactgc tgcgctctcc gtgtacaaca gctgcgtata ttgcaacagc cgtatgctgt
     1261 ttggtctggc acaacagggc aacgcgccaa aagcgctggc gtctgtcgac aaacgcggtg
     1321 taccggttaa cactattctg gtgtctgcgc tggttacggc attgtgcgtg ctgattaact
     1381 accttgcacc ggaatccgca ttcggactgt taatggcact ggtggtatct gcactggtaa
     1441 tcaactgggc gatgattagc ctggcgcata tgaaattccg tcgcgccaag caggaacaag
     1501 gcgtggtaac ttgcttccct gctctgcttt atccgctggg taactggatc tgcctgctgt
     1561 ttatggcggc ggtactggtg attatgctga tgaccccagg aatggcgatt tcggtatacc
     1621 tgatcccggt atggctgatc gtgttaggta tcggctatct gtttaaagag aaaaccgcca
     1681 aagccgtaaa agcgcattaa tctctctacg ccctcacccg tgcagggtga gggcaataat
     1741 ctttacacca acgccccgta aatcgtcagt agcgcaatta ccaccaggac cacgaacgag
     1801 gttttcttcg ccatggaaac cgctgccttc ggcgtctcca ccttatcgac atgcggttca
     1861 cgcgccagag agaactgtgc cagacgtgtt aatacctgat attgcgaagt atggaaatca
     1921 cccagcgaag caaaccaggc cggtaacgct ttctccccat gaccgatcaa ggcatatacc
     1981 acacccgcaa gacgaaccgg cacccaatcc agtacatgaa gcacggcatc aatgccggac
     2041 tgtaaacgat gatgcggcgt ctggtaacgc gccagccagt attgccatgc acgcaaaaac
     2101 gcatacccca tcagcgtaac gggtccccag gttcccccca caatcagcca gaacagcggt
     2161 gcaagataaa aacgaaagtt aatccacagc aatgcatttt gcagctcacg caaatactca
     2221 cgttcatcgc agcctgccgg gacgccgtga atcatggtga gttcgccagc catcgtggca
     2281 cgggcatggc tatcattacg tgaagcagct gtcagataag catgataatg aagacgaact
     2341 ttacctgcgc caatacacag caaaccaatc agcagccaca ccagcagcgt gggaacgttg
     2401 aacaatactc cctgcaatgc gcgtaacagt aaaaaagtca cgcccatcgc aataatggtc
     2461 atgcctaatg tgcgcccgag agaaaaatgt ttcacccgcc gaaagaacgc ttcaagacga
     2521 tgatcaatct gccagtgctc gcccaactta aacaggcgct cgaaaattaa caccagtaag
     2581 gttgtaaata gcgtcatgtt gtctccttgc tgaccagtgc acgaaaccgt gcccaatcaa
     2641 atgcagggcc gggatcggtt ttccgatccg gcgcaatatc acaatggccc gtcatgttat
     2701 tagcgatatc tggatagcga tcaatcagtg cccgcgtaac cgccacaagc tgttgatact
     2761 gcgcatcggt atacgccagc gtatcggtgc cttccagctc aatcccaata gaaaaatcat
     2821 tgcagcgttc gcgcccctga tactgagaga ctccggcatg ccaggcacgt ttatcgaaag
     2881 gaacatactg gactatttca ccatcgcggc gaatcaaaca gtgagcggag acgcgcaaat
     2941 gggcgatctc agcaaagaaa ggatgcgcct gcggatcaat agttccagtg aataatgcgt
     3001 cgatccacgg accgccaaac tcgcctggcg gcaggctaat attgtgcacc accagcaggg
     3061 tgggtgtttc gtcatccggg cggcaatcgt aatgtggtga gggaacgcgg cgcgcgccaa
     3121 ccagccaccc ctgttctaac aacatgcagg atctccttat atgtggtgct aatacccggt
     3181 tcagagtagc atgtttctac cttatgattc gttagctatc tggagtttta acatgccgcc
     3241 tcgccgctat aaccctgaca cccgacgtga cgagctgctg gaacgcatta atctcgatat
     3301 ccccggcgcg gtggcccagg cgctgcggga agatttaggc ggaacagtcg atgccaacaa
     3361 tgacattacg gcaaaacttt taccggaaaa ctctcgctct catgccacag tgatcacacg
     3421 cgagaatggc gtattttgcg gcaaacgctg ggttgaagag gtgtttattc aactggcagg
     3481 cgacgatgtc accataatct ggcatgtgga tgacggcgat gtcatcaacg ccaatgaacc
     3541 cttgttcgaa cttgaaggcc catcccgcgt gctgttaacg ggcgaacgca ctgcacttaa
     3601 ttttgtgcaa accctttcag gggttgccag taaggtacgc cactatgtcg aattactgga
     3661 gggcaccaac acgcagttgt tggatacgcg caaaacctta cccggcctgc gttcagctct
     3721 gaaatacgcg gtactttgcg gcggcggagc gaatcaccgc ctggggcttt ctgatgcctt
     3781 cctgatcaaa gaaaaccata ttattgcctc cggctcagtg cgccaggcgg tcgaaaaagc
     3841 gtcctggctg cacccagatg cgccagtaga agtcgaagta gagaatctgg aagaacttga
     3901 tgaagccctg aaagcaggtg ccgatatcat catgctggat aacttcgaaa cagaacagat
     3961 gcgcgaagcc gtcaaacgca ccaacggcaa ggcgctactg gaagtgtctg gcaacgtcac
     4021 tgacaaaaca ctgcgcgaat ttgccgaaac gggcgtggac tttatctccg tcggtgcgct
     4081 aactaaacac gtgcaggcac tcgacctttc aatgcgtttt cgctgattgc tgacagattt
     4141 atccgctcca tcgcgggcgg ataatctccc ctttcccaac tttcttcgta acgcctcgca
     4201 aatttcttgt ctttcgctgc aacgtaataa aaaactccgg aagctgcttt cccgtttcgt
     4261 tttttgcccc tcgcttgtgc tgatggcgtt tgccaaagta gcgccaacca aatcaaggag
     4321 cgaaacaaat ggacaagcaa cgcggtttta cacttatcga actgatggtg gttattggca
     4381 tcattgccat tttaagcgcc attggtattc ccgcttatca aaactacctg cgcaaagccg
     4441 cactcaccga catgctacaa acctttgtgc cttaccgtac cgccgtagag ttgtgcgcgc
     4501 tggaacatgg tggattagat acctgcgacg gtggcagcaa tggcattccc tcgcctacca
     4561 ccacccgcta tgtttcagcc atgagtgtgg caaagggcgt ggtgtcgctg accgggcaag
     4621 aaagtctcaa tgggctaagc gtcgtcatga caccgggttg ggataacgca aacggcgtca
     4681 ccggctggac gcgcaactgc aatattcaaa gtgacagcgc attgcagcaa gcctgcgaag
     4741 atgtctttcg ctttgatgac gccaactaag gagcggcaat gaatattcca cagctcaccg
     4801 ccctgtgtct gcgttatcag ggagtcttgc tggatgccag cgaagaggtg gttcatgttg
     4861 cggtggtcga tgccccttcg catgagctac tggacgcatt gcatttcgct accaccaaac
     4921 gtattgagat cacctgctgg acgcgccaac aaatggaagg tcacgccagt cgcacccaac
     4981 agacattgcc cgtagctatt catgagaagc atcagcccaa agcagagttg ctgactcgaa
     5041 cgttacaatc tgcgctggaa caacgcgcgt ctgatattca tatcgaacca gcggacaatg
     5101 cctaccgcat ccgcttgcgt atcgacggtg tattgcatcc tttaccggat gttttaccgg
     5161 atgccggagt cgcattaacc gccagattaa aagtgctggg caacctggat attgcggaac
     5221 atcgcctgcc gcaggacggg caattcactg tcgaactggc aggaaacgcc atctcatttc
     5281 gtattgcgac cttaccatgt cggggtggtg aaaaggtggt attaaggttg ttacagcagg
     5341 tgagtcaggc actggatgtc aacacgcttg gaatgcagcc gttacaactg gcggactttg
     5401 ctcatgcctt gcaacaacca cagggactgg tgctggtaac aggccctaca ggcagcggca
     5461 aaacggtcac gctttatagt gccctgcaaa cgctgaatac cgctgacatt aatatttgta
     5521 gcgtcgaaaa tccggttgag atccccatag ccggactaaa ccagacgcaa atccatccgc
     5581 gtgccggact cacctttcag ggcgttttac gtgcgttatt gcgccaggat cctgacgtca
     5641 ttatgatcgg agagatccgc gatggcgaaa cggcagaaat tgccattaac gccgcacaaa
     5701 cgggtcatct ggtattgtcc accctacaca ccaactccac ctgcgaaacg ctggtacgtt
     5761 tacagcaaat gggagtcgcc cgctggatgc tctcatcagc gcttacgctg gtaatagccc
     5821 agcgtctggt acgcaaactt tgcccacatt gtcgccgaca gcaaggggag ccaatccata
     5881 ttccagacaa tgtatggccg tcgccgctgc cccgctggca agcacccggt tgtgtacatt
     5941 gctaccacgg tttttatggt cgcacggcct tatttgaagt tctgcccata acaccggtca
     6001 tacgtcagct tatttccgct aataccgacg ttgaatcgct ggaaacgcac gcccgacagg
     6061 cgggtatgcg aacgcttttt gaaaacggct gtctggccgt ggagcaaggc ttaaccacct
     6121 ttgaagagtt aatccgcgta ttggggatgc cgcatggcga gtaagcaact ctggcgctgg
     6181 cgtggcataa ccggcgacgg caatgcgcaa gatgggatgc tatgggcaga gagccgtgct
     6241 ttgctgctta tggcactaca gcaacagatg gttaccccac taagcctgaa gcgaatcgcc
     6301 atcaattctg cgcagtggcg aggagataaa agcgcggaag tcattcatca actggcgacg
     6361 ctactcaaag ccgggttaac gctttctgaa gggctggcac tgctggcgga acagcatccc
     6421 agtaagcaat ggcaagcgtt gctgcaatcg ctggcgcacg atcttgaaca gggcgttgcc
     6481 ttttccaatg ccttatttcc ctggtcagag gtatttccgc cgctctatca ggcgatgatc
     6541 cgcacgggtg aactgaccgg taagctggat gaatgctgct ttgaactggc gcgtcagcaa
     6601 aaagcccagc gtcagttgac cgacaaagtg aaatcagcgt tacgttatcc cattatcatt
     6661 ttagcgatgg caatcatggt ggttgtggca atgctgcatt ttgtgctgcc ggagtttgct
     6721 gctatttata agaccttcaa caccccacta ccggtactaa cgcaggggat catgacgctg
     6781 gcagacttta gtggcgaatg gggctggctg ctggtgttgt tcggatttct gctggcgata
     6841 gccaataagt tgctgatgcg ccgcccgacc tggcttatcg cgaggcagaa attgctgtta
     6901 cgcatcccga ttatgggttc actgatgcgg ggacaaaaac tcacgcagat ctttacgatt
     6961 ctggcgctga ctcaaagtgc aggcattact tttttacagg gcgtagagag cgtcagagaa
     7021 acaatgcgct gcccgtactg ggtgcaactt ctgacacaaa tccagcacga tatcagtaac
     7081 ggtcaaccca tctggctggc gctaaaaaat gccggtgagt ttagcccact ctgtttgcaa
     7141 ttagtgagaa cagaagaggc atccggctcg ctggatctca tgttagacaa cctcgcccat
     7201 catcatcggg ataacacaat ggcgctggcg gataacctcg cagccttact ggaaccggcg
     7261 ttgctgatca taacgggagg aattatcggt acgctggtgg tggcaatgta tctgccaatt
     7321 ttccatttag gcgatgcgat gagtggaatg ggataacgct ggcgtgttgc tccacgccag
     7381 cgttgggaga ttacaggttg ttgaagatgc ggttttcttg ttccagcaca cggataaatg
     7441 tggtgcgctt ggtcagctct ttcaggcgtg aagccccaac gtatgtacaa gctgaacgca
     7501 ggccgcccaa aatatcacgc gcggtatttt caaccgggcc tcgcagcggc agcttaacgg
     7561 ttttaccttc tgctgcgcga tattccgcaa cgccaccaac gtgacgtttc atcgcagact
     7621 cggagctcat gccgtagaac agcataaatt tctcgccgtt ctcctcaacg atgcgaccgc
     7681 cgctctcttc gtggcccgcc agcatgccgc caagcatgac gaaatcggca ccgccgccaa
     7741 aggctttcgc gacatcgccc ggcgtggtgc agccaccgtc gctgacgatc attccgccca
     7801 gaccgtgcgc agcatcggca cattcgatca ccgcagaaag ttgcggataa ccgacgcctg
     7861 ttttgacgcg agttgtacaa acagaacctg ggccaatgcc aactttaacg atatcagcac
     7921 cggagaggat aagctcctca cacatttcac cagtcactac gttgccagca caaatggttt
     7981 tggtcggcca cgcttcacgc gctttcgcaa cgaactgcac gaagtgttcg gaataaccat
     8041 tcgccacgtc aatacaaacg aagtttaatg ccgggttcag gtcgagaatc tgtttagttt
     8101 tttcgaaatc cgcatcagac gtgccggtag aaaccatcac atgtttcagc acatcagcgg
     8161 aagaattgtt gataaacgct tgccactctt cgaaagaata gtgtttatgc acagcagtca
     8221 aaatatcaaa agaagccagc gcagaggcca tagaaaatgt gcctacggtg tccatatttg
     8281 cggcgataat cggcacgccg gaccagctct gacctgaatg tttgaaggtg aattgacgtt
     8341 ccagttcaac atcggaacgg cttttaagag tggagcgttt agggcggatg agaacgtctt
     8401 taaaacctaa cttcagatct tcttcaatac gcatgtgcgg attcctgggg ttaatggcta
     8461 taagggtaag agcgcaactc cagtgacgct atcatacgca ctaatcaatg cggcgcaaga
     8521 ctgcgaaatt gtcttttttt acgctacaat cccataaatt tacctggtaa atagtgagcg
     8581 aacgtttcat caatttcgct ttcgtgcgct catttttaac gttttgataa tcatgatgaa
     8641 ttcccgggaa tataaattat gaggtatata gttgccttaa cgggaggcat tggcagtggc
     8701 aagagtaccg ttgccaatgc gtttgctgat ctcggaatta acgtcattga tgccgatatt
     8761 attgcgcgtc aggtggttga accaggtgca cctgcgctac atgccattgc tgatcacttt
     8821 ggcgctaaca tgattgctgc tgatggaaca ttgcagcgcc gggccttgcg cgagcggatc
     8881 ttcgccaacc cggaagagaa aaactggctt aacgccctgc tgcatccgct gattcagcaa
     8941 gagacgcaac accagatcca gcaagcaacc tccccctatg tactgtgggt tgtgccattg
     9001 ctggtagaaa actcactgta taaaaaagcg aatcgagtgc tggtggtgga tgtcagccca
     9061 gaaacgcaac ttaagcgcac catgcagcgc gatgatgtaa ctcgcgagca tgtcgaacaa
     9121 atccttgctg ctcaggcacc gcgcgaagct cgccttgccg tggcagatga cgtcattgat
     9181 aataacggcg caccggatgc tatcgcatcg gatgttgccc gcctgcacgc acactatttg
     9241 cagcttgcgt cgcagtttgt ctcacaggaa aaaccgtaat gcagacccag gtcctttttg
     9301 aacatccact aaatgaaaaa atgcgtacat ggctgcgcat tgagttttta attcaacaac
     9361 tcaccgtaaa tttgcccgtc gttgactacg ctggcgctct gcatttcttc cgcaatgtca
     9421 gtgaattgct ggatgttttc gagcgcggcg aagtccgcac tgagttgttg aaagaacttg
     9481 accggcagca acgtaaactc cagacctgga ttggcgtgcc tggcgtggac cagagccgta
     9541 ttgaagcatt aattcagcag ttaaaagcgg cggggagcgt attaatttcc gcgccccgta
     9601 tcgggcaatt tctgcgtgaa gatcgtttga ttgccctggt gcgtcagcga ctgagcatcc
     9661 ccggcggctg ttgtagcttt gatttaccta cattgcacat ttggctgcat ctaccccagg
     9721 cgcagcgcga cagccaggta gaaacctgga ttgccagcct gaacccgctc acccaggcac
     9781 ttaccatggt gctggattta attcgccagt cggccccctt ccgtaaacaa accagcctga
     9841 atggttttta tcaggataac ggtggcgatg ccgacttgct gcgcctgaat ctgtcactcg
     9901 attcacagct ttatccgcaa atttccggtc ataagagccg ttttgccatt cgttttatgc
     9961 cgctggacag tgaaaacgga caggtaccgg aacgtctgga tttcgaactg gcctgttgct
    10021 aaggagtaaa aatgtcagaa actattacgg tgaattgccc aacctgcggg aaaacggtgg
    10081 tgtggggtga aatcagcccg tttcggccat tttgctccaa acgttgtcag ctgatcgacc
    10141 tcggagaatg ggctgctgaa gaaaaacgaa tccccagcag tggcgatctt tccgaaagcg
    10201 atgactggag cgaagagcca aagcagtgac attttatctg atgcgacgct ggcctgataa
    10261 gacgcgggca gcgtcgcatc aggcatcgtg tgcaaatgtc ggatgcggcg ggaacgcctt
    10321 atccgaccta caagacctta catatcaaca tgttaaaatt gtagatactg ataagacgcg
    10381 ccagcgtcgc atcgggcatc ttgtgcaaat gttggatgcg gcataaacgc ctatccgaca
    10441 tacacacgct taagcttcgc aagtaccggt tcattggctg gcggaaaatc atcggcattc
    10501 agaccgacca gcgacatcca ctcaccgggt tgcccttctt taccccacgg ctccccttcc
    10561 cagctttcga ccagccagaa ccacagtgtt atatgcctgt ccgggaattc atattccagt
    10621 atttcaaata gcgaaagatg ttgtggggta atcccgactt cttcctgaag ttcacgcacc
    10681 accgcctgtt ccggcgtttc acccatttca attttaccgc cgggaaactc cagtttattc
    10741 gccatgtgcg catctgctgc gcgacgggtt ataaagattt cattgttctc gttgcgaata
    10801 atacctaccg caatttgcag ctttttcatt gtcttaaacc tataaaaaag gcgcagaatc
    10861 ctgcgccttt tacttcaact gttagctttt attgcaggcg gccatggcac tgcttatatt
    10921 ttttaccaga accacacggg caaggatcgt tacgtcctac tttgcgttca ccggtttgcg
    10981 ccgccagtgc agctgcggca gcagagtcgt catcctgatg gctaagctgc tgcatttgcg
    11041 ctaaacgctc ggcttccata cgacgctgtt gttccagctc ctcaacctct tcaggcatac
    11101 gtacctgaac tttgctcagc gtgctgataa cttcatattt taacgactcc agcatcgctg
    11161 caaacatgga gaacgattca cgtttgtatt cctgcttcgg atctttctgt gcatagccac
    11221 gcaggtggat accctgacgc agatagtcca tcgcagccag gtgctctttc cacagagagt
    11281 caagcgtttg cagcatgacg cctttctcga agtgacgcat catctcagca ccaaccactt
    11341 cttctttacg ctgatacact tcgatggact gcgccagaat gcgctcacgc agcgtctctt
    11401 catgcagttc tggttcttta tccagccact cggcaattgg caaatcgagg tcgaaatcgt
    11461 tcttcagacg ttcctgcagc cccggaatat cccacatttc ttccagcgac tgtggcggaa
    11521 tgtaggcatc aatggtcgct ttgaacacat cttcacgaat gctgttgatg gtttcgctca
    11581 catcgctgac atccagcagt tcgttacgct gggagtaaat ggcgcgacgc tgatcgttag
    11641 ccacgtcatc atattccagc agttgcttac gaatgtcgaa gttacggctt tcaactttac
    11701 gctgggcgtt ggcaatcgct ttggtcaccc acgggtgctc aatggcttcg cctggcttca
    11761 tacccagttt acgcatcatg ccggatactc ggtcggaagc aaaaatacgc atcagcgcat
    11821 cttccatcga caggtagaaa cgggaagaac cagcatcccc ctgacgacca gaacgaccgc
    11881 gcagctggtt atcgatacga cgggattcgt gacgttcagt accgatgata tgcaggccac
    11941 ctgctgccag taccgcatcg tgacgtacct gccagtcggc tttaattttt tcaatttgct
    12001 ctgcagtcgg attttccagc gcggcaactt ctgcctgcca gctaccaccg agcacaatat
    12061 cggtaccacg acccgccatg ttggtcgcga tagtcaccgc agccgggtaa cctgcctgag
    12121 caacaatcgc cgcttcgttg gcatggaatt tggcgttcag gacgttgtgc ttaataccgg
    12181 ctttggtcag ttcatttgac accagctccg atttttcgat ggagattgta cccaccagca
    12241 ccggctggcc tttcgcagtg cgttctttga tatcttcaat gatcgcctga attttttccg
    12301 cttcagtcat gtagaccagg tccggcagat ctttacgaat cattggacgg ttggtcggaa
    12361 caacgacagt atccagctta tagatggagc tgaattcgaa agcttcggta tcagcagtac
    12421 cagtcatccc cgccagtttt tcatacagac ggaagtagtt ctggaaggtg atcgaagcca
    12481 gcgtttggtt ttcgttctgg atctgcacac cttctttcgc ttccacagcc tggtgcagac
    12541 catcggacca gcgacggccc tgcatggtac gaccggtgtg ttcgtcaacg atgataactt
    12601 caccatcttt aacgatgtag tcgacgtcgc gggtaaacag cgcatgagcg cgcagcgccg
    12661 ccgttacgtg gtgcatcagc atgatgttgg ccggagagta cagagactca ccttcatcca
    12721 tgatgccttc tttaaccagc agttcttcaa tcagaaccag accacgttcg gtcaggttca
    12781 cctggcgaga tttttcatcc accgagaagt ggccttcgcc ctggaaggtt tcggagtctt
    12841 ctttttcctg acggatcagg tgtggaataa ttttattcac gcgtttatac atttccgagc
    12901 tgtcttctgc cgggccggaa atgatcagcg gtgtacgcgc ttcatcgatc aggatggagt
    12961 ccacttcgtc caccagcgca tagtgcagtt tacgctgtac acgttcttca ggactgaacg
    13021 ccatgttgtc gcgcaggtag tcaaagccgt attcgttgtt cgtaccgtaa gtaatgtcag
    13081 ctgcgtaagc ttcacgcttt gccggtgctg gcatgcccgg caggttgata ccaactgtca
    13141 ggccaagaaa ttcaaacagc ggacggttgt tttcggcgtc acgttgcgcc aggtagtcgt
    13201 tgacggtaac tacgtgcacg cctttaccgg ttagtgcgtt caggtaagca ggcagcgttg
    13261 cggtcagggt tttaccttca ccggtacgca tttcggcgat gcagcgttcg ttaagaacca
    13321 taccgccgag taactgaacg tcgaagtgac gcataccaaa gacgcgctta cttgcctcac
    13381 gcaccacggc gaaagcttcc gggatcagat tttccagcac ttcgcctttt tccagacgtg
    13441 cacgaaactc tgcggttttc cctttcagtt cttcgtcgga gagtttttcc atctccggtt
    13501 ccatggcatt gatgatgttg accactttgc gcatccggcg cagggtgcga tcgttacgac
    13561 taccgaaaac tttagttaat aatttgatta gcataataaa atctcaaacg ccccgcgttg
    13621 cggagttaat aaaatgaagt aacggtttat tgttgttagc tgaggcgttg agggccagca
    13681 cggatgcctt gcgcctggct tatccagacg ggcgtgctga attttgcttg cggggtaaaa
    13741 tgcgcataat caatgcgata acccttttca gacggcgtgc cttcctgggt caacagcgcg
    13801 ctgagcgtat ccagtaatgc aagatgttgc gcctgaagag gcaaagattc ttcagcaacg
    13861 ggcagcgttt gcggtgccat tgcgaaggaa agatgacgga ttaccgtgcg aatggcatgt
    13921 tgatgccagt aatcaacgga atagttcgaa ttcgggcgac gcgtgttcgc ttccagcaag
    13981 gccaattgac caaagttaac tttggctgaa ggctcgtggt tgcgggttgt cgcttttgcg
    14041 ggcgcgtttg gttcggcggc gttgctgagc gcaggcaaac ctaaactcgc cgcgaccatc
    14101 cctaataaga gatgcggcca gaagtagcgt ttaccaaact gtcgccagcg cgtcagtatt
    14161 ccactcacgt tattgccatc ccgatcgtat tcaaacgtat ttagcgcaca aatcttatca
    14221 ttaaagcctg aagtccacag cacgaatgaa gataagttgt gcgtaaaagt gcttaagaaa
    14281 acagcgttcg caccgttttt tatcgttctt ataaaattca aaaaaaaacg actggttcac
    14341 ctggccggag agagtgccag atttgccagt cgaattttat acgacagtat aaatgtcgtt
    14401 atgccagtac ggctgaaggc gctttgaagg ccaacggcag ttctgcgtcg tcctggaagg
    14461 tcacatattc ccaggcttcc tgtttcgcca ggacagcctg cagcagtttg ttattcagtg
    14521 catgaccaga tttataagcg gtaaatgcac caataatatt gtgaccacac atgaacaagt
    14581 caccgatcgc atcgagcatt ttgtgacgca caaattcgtc ttcaaaacgc aggccgtctt
    14641 cgttcagtac gcgataatcg tcaacaacga tggcacaatc gaagctgccg cccaggcaca
    14701 aaccacggga ctgcagatat tcgatatcac gcatgaaacc gaacgtacgc gcacggctga
    14761 tctggcgcat aaacgcatca gcggagaagt tcatcgcata gcgctggttg ctggaatcaa
    14821 tagccggatg gttaaaatcg atggtgaaat ccagcgaaaa accattgtac ggcttaaatt
    14881 cagcccactt atcgccatct tcgacacgaa cagtctcttt gatgcgaaca aatttcttgg
    14941 cgcagttcag ctcgtcgata ccagcgtcaa gcagcaggta tacaaacgga gcggcgctgc
    15001 cgtccatgat cgggatttcc ggcgcgttaa cttcgataac aatgttatcg atgcccaggc
    15061 ccgcgagagc agcattgagg tgctctacgg ttgaaatccg tacatcatgc tcgttgacca
    15121 gacacgtaca gagcatggta tcacgcacag atttggcatc ggccgggaaa tctaccggtg
    15181 gattcaagtc ggtgcgacga tagatgaccc cggtgttggc cggcgcaggg cgtaacgtca
    15241 gggtgacttt cttgccggta tgtaaaccga cacccgtcgc ctgaacgata cgtttaagtg
    15301 tcctttgttt gatcatcgta ttatctcgcc aaattaccta tccaaccgaa gtgtactata
    15361 cattcggcgg gccagtttag cacaaagagc ctcgaaaccc aaattccagt caattcttaa
    15421 tcagcttgct tacgcaggaa tgctgggata tccagataat ccggctcttt cgcagtttgc
    15481 ggcgcattgt cattcacgac tttagcaacc ggcttctgct cctgggtcag cggagccatc
    15541 ccgtgctgct ggtagcgatc catcactggc tgctgaacct gcttattggt caccagagtg
    15601 atttcaggac gtttgtccat gccgatacct gtcgcaacaa cagttacgcg cagctcgtca
    15661 ttcatatccg ggtcaagaga agtaccgata accacagtcg cgttgtcgga agcaaatgca
    15721 cggatggtgt tacctaccgt ttcgaactca tccagacgca ggtcgaagcc cgctgtgatg
    15781 tttaccagta cgccgcgtgc gccagacagg tcgatatctt ccagcagcgg agaagagata
    15841 gccatttcag cagcttcttc cgcacgatct tcaccgctcg ccacaccaga acccatcatt
    15901 gcgtagccca tttcagacat tacggtgcgt acgtctgcaa agtccacgtt catcaaaccc
    15961 ggacgagtaa tcagttcggc gataccttgc acagcgcctt tgagtacgtc gttcgctgcg
    16021 ccaaacgcat ccagcaggga gataccgcgg cccagaactt tcagcagttt gtcgttcggg
    16081 atagtgatca gagagtccac atgcttggac agttcagtga tcccctgctc cgcgaatgcc
    16141 atacgcttct tgccttcaaa gttgaagggc ttagtgacga cagcaacggt caggataccc
    16201 aaatcttttg ccacttcagc gacgactgga gctgcgcctg taccggtacc accacccata
    16261 cccgcagcaa taaagaccat gtctgcacct tccagcgccg cacgcagtgc atcgcgatcc
    16321 tcatcagccg cattgcggcc aacttctgga ttagcgccag ctcccagtcc tttggtgata
    16381 ccgctaccga tttgaatcgt ctgtccaacc gctgttttac gcagcgcttg tgcatcggta
    16441 tttaccgcga agaattcaac accttcaatg cgctcgcgca ccatgtgttc aacagcatta
    16501 ccgccgccgc cgccgacgcc gatgacttta atcaccgcgt cattggtaag ttccattggt
    16561 tcaaacatag tttctctccg atttgtgcct gtcgcctgag accgtaatca ttgtcggcct
    16621 cataaaaatt aaaactcttt tcgcagccaa ctattgagtc gcttgatcca cgagccaact
    16681 gatgctgtaa cacgtttttc tacttcagct tcaccgttaa gatgtgactc tttcccgtaa
    16741 tgaagcaatc ccaccgccgt cgaataatac ggctcctgag cataatccgt taaaccggta
    16801 atgttcagcg gcgcgccgat acgcacctgc gtatgaaaca cgcgctgagc acaggctgca
    16861 agaccttcaa tctgcgctgc gccaccggtt aatacaatgc ctgccgccag gtgatgttta
    16921 accccttgtt ggcgaagctt ttcctgcaac tgcaatatct cttcgttgac caggttgagc
    16981 agctcggtat agcgcggctc gatcacctct gccagggtct gacgttgcag actccgcggc
    17041 ggacgaccgc ctacgctcgg cacttccacg ctctcatctt ttccaacgat ggaacccagc
    17101 gcacaaccgt ggcgaacttt aatcgcttcg gcgtcgcttg gcggcgtgcc aaaggcgtaa
    17161 gcgatatcac tggtcacgac attgccagca taaggaatta ccttagtgtg gcgcaatgcc
    17221 ccaccggtat aaacggcgat atccattgta ccaccaccga tatcgacgac gcagacaccc
    17281 agttcacgtt catcttccgt caataccgaa taacttgatg ccagtccggc aaatatcagt
    17341 tggtcaactt tcagcccaca acgttcaacc gctttgacga tgtttttcgc catatcgttg
    17401 tgacatgtga tcaggtgcac ttttgcctgc atccgcacgc ccgaaagtcc taccggattc
    17461 ttgatccctt cctgatagtc aatcgcatac tcttgcggga tcacatgcag cacacggtgt
    17521 tcatcgcgca cacgcaccga tttcgcggta tggacaacgt tttccacatc ttcttgcgtc
    17581 acttcttctt cagaaatagg caccatacca atttcattct ggcagctgat gtgcttacca
    17641 gaaagcgcca gatataccga agagatctga caatctgcca tcaattctgc ctggtcaatg
    17701 gcgcgctgta cgcacttgac cacggattcg aggtcgttca ccccgccttt atccatacca
    17761 cgcgacgggc agctgcccac gccaatgata ttgaccatac cgtcgggcag aacttcccct
    17821 actaaagcgg caaccttcgc ggtaccaatc tccagtccta ctaccagttt tctgtccgtc
    17881 gccttaatca ttgttgttct gcctgtgcct gattttgttg ctgagtagat tcctctggcg
    17941 gcaagggcgc ccagcctact gccgctccag agtcataacg caaatcaacg tagctaatcc
    18001 gtttgccatc ggtttgcgcc tgctgctgta aaaccggata aagttctaca aagcgagcca
    18061 aacgtttcat cgtatcgccc cggccaagat tgagcttaat atcgttattc agcgtcaact
    18121 gccaggaacg ccgcgcggtc atcgccgctt ccttcagagt aaatctgtcc tttgccagca
    18181 tctgccccat ttcgcgatag ccctgcaaca cttcattggc gctgccttcc gggccataca
    18241 gcattggaag cacctgcttg ctggtgcgat ctggcggcac gctgaaagtg tttccttccg
    18301 cgtctaccat atgttgatca ttccaccgcg caatcggcac atattcaacc agatgaatct
    18361 tcaattcatc aggccactgc tttctgacgc tcacctgctt aatccacggc aggcgttgtt
    18421 ctatttgcgt ctggatgatg ttgacatcct gggtcataaa ggtacctggc tcacccaatg
    18481 ccaggatcga ctgccggata tcgtcattac gcgtgtaatg gcgttcaccg gtcaacacca
    18541 gctttgagag cggcaggcgt tgcgcatctt ccatccagcc caacacgacc cagccgctca
    18601 ccaacactgt cgttaaaacg gtcagcagga aaaggatccc cgccagacgc gttccattat
    18661 tgcggcgaga agaaacctct tcttcgctgt ttcgcgtgtt cagagcagcc tgcgacatat
    18721 tagtccgcca gttccagaat tcgtactacc aactgcgaga agctcatacc tgcctgacgt
    18781 gccgccatcg gcaccaggct gtggctggtc atacccggtg aggtattggc ttccagcaga
    18841 taaaactgtc catcgctgtc cagcataacg tcaatacgtc cccatccttt gcaacctaac
    18901 gtcgtccatg ctttcagcac taatgcctgc aaattggcct cttgtgacgc ttccagacct
    18961 gcggggcaga aatactgtgt ctcatcagag agatacttcg cctcataatc atagaaggtt
    19021 ccggacggtt gaatacgtat tgacggtaaa atttcttcac cgagtatcgc aaccgtgaac
    19081 tccggcccac ttagccattt ttcaatcaat acttcttcat cgtgctgaaa tgccaatctt
    19141 aatgcatctt gtagagcatt ttctgctact acttttgaca ttcccacact ggaaccttcg
    19201 cggctcggct taacgataac cggcaaaccc agagcagaaa tttctgctaa ctgcttatca
    19261 ctcaggcctt tttcaaactc tgcgcgggtt aacgctaccc acggagcgac cggtaaaccg
    19321 gcaccttgcc acagaagttt gctgcgtagt ttatccattg aaagcgcaga tgccatcact
    19381 ccgctgccgg tataaggcaa gcccatcagc tcgagcatgc cctgcagcgt gccatcttca
    19441 ccgccgcgac cgtgtagcgc gataaacact ttctgaaagc ccatcgactt cagttgcgtc
    19501 acgtcgactt ctttcgggtc gacaggattc gcgtcaatac cgccttcacg cagcccggct
    19561 aacaccgctg cgccagaatt cagagaaact tcccgctcag cggaggtccc acccaacagg
    19621 accgcgattt tatcagtcat gttgttcttc ctccggagtt tgcggcttca gtttgatttc
    19681 agctaaagaa cgggcaattt ttccgatatt accagccccc tgaacgagaa tcaggtcgtt
    19741 accggttaat accggagcca gcatctcggc tacctgcgcc gggtccggca ccagaatggg
    19801 atcaattttc ccacgtccac gaattgtgcg acacagcgaa cggctgtccg ctcccggaat
    19861 tggcgcttcg ccagccggat acacttccag catcaacagg gtatcaacct gcgtcagcac
    19921 attggcgaaa tcatcataca ggtcgcgcgt acgggtaaaa cggtgcggct gaaacagcat
    19981 taccaggttt ttatccggcc agcctgcacg cgccgcttta atggtggcgt ccacttccgt
    20041 cgggtggtgg ccgtagtcat cgaccagcat tgccgtaccg cttttaccat tcacaggctc
    20101 cagcgggaat tcaccgagga aatcaaaacg gcgaccagtc ccctggaagc tctccagcgc
    20161 acgcaaaata gcctcgtcgt caattccctc ttccgtagca accgcaaccg cagctgcggc
    20221 gttcagcgcg ttatgacggc ctggcgcatt cagggtgacg cgcatcggct ctttgtcctg
    20281 gcgcagcagc gtaaagtgcc cctgcgggcc aatctgctga taatcttcta cacgcacgtc
    20341 ggcatcttcg ctgaagccgt aagtcgtggt ctgacgtccc acacgcggta ataattcgcg
    20401 gatcaccgga tcatcaacac acatcaccgc acgaccgtaa aacggcaggt tgtgcagaaa
    20461 attaataaag gtctgtttta aattctcaaa gtcgccctgg taggtatcca tgtggtcggc
    20521 ttcgatattg gtgacaatcg ccaccatcgg ttgcagatgc aggaacgatg catcactctc
    20581 atctgcttcg gcaatcaggt accgaccatg ccccaaacgc gcatgaacgc ccgccgcttt
    20641 taccagccca ccgttaacga aggttgggtc gagtcccgct tctgcgtaga tgctggaaac
    20701 catcgcagtg gttgtcgttt tgccgtgcgt tccggcaatg gcgatgccat gacgaaaacg
    20761 cattaactca gccagcattt cggcacgacg gatcaccgga atacgcgctt catgagctgc
    20821 gacaatttcc gggttatcgg cagaaatcgc gctggaaaca acgaccacgc tggcatcacg
    20881 tacgttttcc gggcgatggt tgaaataaat cgtcgcccca agattcatta actgctgcgt
    20941 gaccggattt ggcgctaaat cggaaccact gatctgataa ccttcattgg ccagaacttc
    21001 ggcaataccg cccataccgg caccaccgat gccgacaaaa tgtatgtgcc gaacgcgacg
    21061 catttcgggc acgatggaac gcagttttgc caattgttgt gtattcattc tttacgccat
    21121 taacttctta aattcatacg atgcaaaagg catcgctaca attacgcccg ggcagcccgg
    21181 ctcacttcat ttgccactcg ctcggtggca tccggaatgg atgcggcgcg ggcgcgttct
    21241 gccatggtta ataaggtttc tcgcgaccac ccggccaggg tgttggcgac agcatccacg
    21301 ctaagctgtg gctgctcgat aattttggct gcgcccgctt tttccagcgg tagcgcattc
    21361 cagtattgct ggcggtcttt atgttgaaac ggcacaaaca acgccggtag tcctgccgcg
    21421 gcgatttcac tcaccgttaa cgcaccggag cggcaaacga cgacatccgc ccacgcatac
    21481 gccgccgcca tatcatcaat aaattccgtc actttatgct gcggttgccc cgcttcggca
    21541 tacgcctgtt caacggattg ttgcgaacct ttaccgctct gatgccagat agtgactgaa
    21601 tcacccagtt tcgcagcaac ctgcggcatt gtctggttaa gaatgcgtgc gccctgggaa
    21661 ccacccacta ccaacacacg aaccggacct tcacgtccag ccaaacgttg ctgcggcaac
    21721 ggcagcgcca gcacatcggt acgcaccggg ttacccacca cttccgcatt agggaaagcg
    21781 cctggaaacg cctgcatcac tttggtggca atcttcgcca gccatttatt ggttaatccc
    21841 gcaataccgt tttgttcatg aagcacaacc ggaataccta acgaccacgc ggccagacca
    21901 cctggacctg atacgtagcc gcccataccg agcaccacgt caggtttgta cgctttcata
    21961 atcgcccgcg cctgacgcca ggcgttgaag atacgcagcg gggcagctat cagtgctttt
    22021 ataccttttc cacgcagacc agagatacga atgaaatcaa tttcgatgcc atgttttggc
    22081 actaagtccg cttccatacg gtcggcagtc cccagccagc gaacttgcca accctgagcc
    22141 attagatggt gcgcaaccgc cagtcccggg aatacatgtc caccggttcc gcctgccatc
    22201 accattaatc gcttcccttg accactcatc gtgaacctcg tacaaacgcc tgcgctttct
    22261 ccagacgcgt ttcataatca atacgcaaca gcatcatgat ggctgtcgac ataatcagta
    22321 agctcgaacc accgtaactg atcagcggca atgtcagacc tttggtcggt aacatccccg
    22381 ccgccgcgcc tacgttaacc agcgcctgga aactaaacca gataccaata gaacaggcga
    22441 gaaaaccgga aaaacggtgg tcaatttcta atgctttgcg gccaatcgac atcgcgcgaa
    22501 aagcgacgaa gaataccatt aaaagtgcca gcaccacacc gacatacccc agttcttcgc
    22561 cgataatggc gaaaataaag tcagtgtgcg cttccggcag atactccagt ttttgtaccg
    22621 agttacctaa accttgtccc cacagttcac cgcgaccaaa cgccatcaac gattgcgtta
    22681 actgatagcc gctgccaaag ggatcttccc acggattcca gaatgcggta acacggcgga
    22741 tacggtacgg ttcggcgagg atcagcaaca caaccgctga aatgcccata ccgataatgg
    22801 caatgaactg ccacaatttc gctcccgcca ggaacaacat cgccagcgta gtcacaaaca
    22861 acaccaccac cgtaccaagg tctggctgtg ccagcagtaa cactgccaac accagaatta
    22921 cgcccatcgg tttcaggaag ccgcgcaggt tattacgtac ttcgtcgcct ttacgcacca
    22981 gatagttggc gatatagcaa aacagcgaca gttttgtcag ctccgcaggc tggatacgca
    23041 gcaaaccgag atcgatccaa cgcgatgccc ctttaaccga gctacccact accagaacga
    23101 tcatcagcag gatgatagat ccgagcagca tcgtggcact gtagcgttgc cagaactcca
    23161 tcggcagacg cagcgtaatg atcgccagaa tgaatgccag gatcagatag acgccatcac
    23221 gcttcgcgaa gaagaacgga tcgttggtta agcgttgccc tatgggcatt gacgccgagg
    23281 tcaccatgat aaagccaatc gccgcgaggc cgaaggtcag ccacagtaag gtgcgatcgt
    23341 acatgatcag gctgtcggta tctttttccc gcgagcccat cacccagccc tttagcgccg
    23401 tggagatcca gaccagaata ctgaatcctg gcaggcgcgg cattttcagg cgagggagag
    23461 ataaacgcat caacctaact ccttcgccag acgggcaaac tcattgcctc gttgttcaaa
    23521 gttcttgaat tgatcaaggc tggcacaggc cggggagagc agaaccatat cgcccggctg
    23581 aacacgcgga gccagcaagc gcatcgcctg ttccatggtt tcggtttgtt ctgccacttc
    23641 cgggcgtagc gccgccagct gcgcgccgtc ccgaccgaaa caatacagac gtacattatc
    23701 gccattcagg taacgcgcca gtgggctaaa gtccgccgat ttaccatcgc cacccagcaa
    23761 caaatgcagc gtgccgtcta catgcaggcc attcagcgcc gcttccgtac tgccgacgtt
    23821 ggtcgctttt gaatcgttaa cccagcgcac accgttatgc tccagcacaa cttcaaagcg
    23881 atgcggcaga ccagtgaatg tggttaacgc tttcaggctg ctggcacgcg gtaaccctgc
    23941 ggcatctgcc agcgccagcg ccgccagcgc attggtgtag ttatgctgcc cggaaagttt
    24001 catttctttc acgttcagca ctttctcgcc tttcacccgc agccaggttt caccctgctg
    24061 atgattcagg tgatagtcgc ccatgttgac gccaaagctg acgcagcgtt catccgcacc
    24121 gcgaatcggc attgttaagg catcatcagc attaaccacg caaatttttg cgttttcgta
    24181 aatgcgcagt tttgctgcac gatactgttg taaaccaaac ggatagcgat ccatatgatc
    24241 ttcagtcacg ttcagaatgg tcgctgctac cgcctgtaaa ctggaggtgg tttcaagctg
    24301 gaagctcgac agctccagca cgtacagttc gcactcatca tccagcagca tcaacgcagg
    24361 caggccaata ttgccaccca caccaacgtt aacccccgcc gctttcgcca tttcacccac
    24421 tagcgtggtg accgtgcttt tgccgttaga accggtgacc gccacaatcg gtgcttgtgc
    24481 ttcacgacag aacagctcga tatcgccaac gatttcgatt ccggcatcag cggcagcgct
    24541 taaggatgga tgcgccagtg caataccggg actggcgaca atcagatcgg ccgccatcag
    24601 ccattcatca ttcagaccgc ccgtgtggcg ttctacagct tcgggtaatt tatccaggcc
    24661 aggcggtgtc atacgcgtat ccataacgcg cggcgtcaca ccgcgagcga ggaaaaagtc
    24721 cacgcaagaa agcccggtga ggcccaggcc gataatgacg acatttttac cctggtaatc
    24781 agccatgatt aacgtacctt cagcgttgcc agaccaatca ggaccagcat cagcgaaata
    24841 atccagaaac gtacaatgac gcgcggttcc ggccagcctt tcagttcata gtggtgatga
    24901 atcggtgcca tgcggaaaat acgttgtccg cgcagtttaa aggagccgac ctgcaggatg
    24961 acggaaagtg tttctaccac gaacacgccc cccataatca ccagcaggaa ttcctgacgc
    25021 agcagtacgg cgataatacc taacgcacca cctaacgcca gcgaaccgac atcgcccata
    25081 aagacctgcg ccggataggt gttaaaccac aggaagccca gccctgcccc gactatcgcg
    25141 gtacagacaa tgaccagttc cccggcgtgt cgtagatacg gtatgtgtaa atagctggca
    25201 aagttcatgt tgccggtcgc ccacgccacc agcgcaaaac caccggcgac aaatacggtc
    25261 ggcataattg ccagaccgtc gagaccatca gtcaggttta ccgcgttgcc tgtacccaca
    25321 atgacgaagt aagccagcag aatgtagaac agccccagtt gcggcatcac atctttaaaa
    25381 aatgggacca ccagctgcgt tgcgggcgtg tctttgccaa caaggtacag cgcgaaggcg
    25441 acacccagcg caatgaccga catccagaaa tacttccaac gagcgatcaa ccctttggtg
    25501 tctttacgca ctactttgcg ataatcatca acaaagccaa taacaccgta acctaccagc
    25561 actaccaaca cgcaccagac gtacggattg gacgggtaag cccacagcag tacggagatc
    25621 acaatcgctg tcaggatcat aatcccgccc atggtcggcg taccgcgctt gctgaagtgt
    25681 gattcaggac cgtcgttacg caccacctga ccaaaggaaa gtttttgcaa atgagcaatc
    25741 atgcgcgggc ccatccacaa tgagatgaac agcgcagtca gcaggctgac gatggcgcga
    25801 aacgtcagat aggaaaagac gttaaagccg gaataatatt tgaccaaatg ttcggccagc
    25861 caaactaaca tgtcccattc tcctgtaaag cgcgtactac ctcttccatg gcagcactac
    25921 gtgaaccctt aactaaaatc gtaattacct gttgctcagc aatcagtgat ttaagacgcg
    25981 cgataagcgc agttttatcg gaaaaatgtt cgccaacacc gctggcagtg ctgatagcat
    26041 ggctttgttt gcccacgctt aacacgcggt caataccagc tgcttttgcc gcttcgccca
    26101 cctgtatatg gcaggcttcg ctttcagcgc ccagttccgc catatcgccc accaccagca
    26161 cgcggtagcc cggcatttca gccagtacct ggactgctgc ggtcattgaa ccgacattgg
    26221 cgttgtagga gtcgtcgagc agcaactggt tttccgccag ttggatgggg aacagacggc
    26281 ctggaacagc tttcagattt gccagccccg ctttgatagc atcaagcgtt gcgcccacgg
    26341 acatggagag cgcagcggct gccagcgcat tcgcaatatt gtgacgcccc ggcaacggca
    26401 gcagaacatc cacgctaccg gttggggttt gcagggtaaa ttccgtaccg tgcgaagtca
    26461 catggatgtt ggtggcggtg aaatcgctgt tggcggcatt aggtgagaaa cgccacactt
    26521 tgcgtgagcc aattacgctc tgccagttca gccagtcgtt gttgtcggcg ttcataatgg
    26581 cgataccgtt ttccggcagg ccgctaaaga tttcaccttt cgctttcgcg acacccgcaa
    26641 gcgagccaaa accttccaga tgcgccgctg ccaggttgtt gaccagcgcc gcttccgggc
    26701 gagtcagact cacagtccag gcaatttcgc cctgatggtt cgcgccaagt tcaattactg
    26761 cgtaatcgta ctctggcgtt aagcgcaaca gcgtcatcgg tacaccgatg tcgttgttga
    26821 gattgcctgc cgtataaagc gtgttgccac actggcttaa aatcgctgcc gtcatctctt
    26881 taacggaggt tttgccggag gaaccggtca gagcaaccac gcgcgccgga acttgctggc
    26941 gaacccatgc agccagttca ccaaacgcca gacgagtatc cttgacgatt acctgcggca
    27001 gatcgatatc cagcggacgg ttaaccagta gtgcacccgc accgccagct ttcgcctggt
    27061 cagcaaaatc atgggcatcg aaacgttcgc ctttcagggc aacaaacagg caacccggcg
    27121 tcagttttcg tgtgtctgtg gttacagcat caagggtgat atctgcccct tttagttcac
    27181 cgttgagaat atcggtaagt tggctaaggg ttacgctaat catgcaatca cccccagcag
    27241 acgcgccacc gtgacgcgat cggagtagtc cagacgctga ttgccaacaa tctggtaatc
    27301 ctcatgacct ttgcccgcga ccagtaccac atcattctct ttagcctgca taacggcgca
    27361 agtcaccgct tcagcacggc cttccatcac tttggcatgt ccggcatcta acattcccgc
    27421 cagaatatcg ttgatgatgg cacgcggttc ttcggtacgc gggttatcgt ccgtcaccac
    27481 cgccacgtca gcgaactctt cagcaattgc gcccatcagt ggacgtttgc ctttatcacg
    27541 atcgccacca cagccaaaga cgcaccacag cttgcccgca cagtgcaggc gcgccgcctg
    27601 taaggctttt tccagtgcat ccggtgtatg cgcgtaatcc accactaccg tcggtttgcc
    27661 tggcgcagtg aacacttcca tacgtccaca aaccggctgc aggcgtgcgg cagttttcag
    27721 caggtcagcc aacggatagc ccagcgccag cagcgtcgcc agcgccagca gcaagttgct
    27781 gacgttaaaa gcgcccatca gatggctttc aatttcgcca tcgccccagc ttgagctaaa
    27841 gcgaatcgtc gcaccgctgt cgtgatagtt cacttctgtc gctttcaacc agcgtccgtg
    27901 acagttcgga ttgatatgat cttccattga taccgcaacc gcgtccggca gttttgccag
    27961 ccagcggcgg cccacttcat cgtcggcgtt aataatcgcc tgaccgcaat gatgctcaga
    28021 ataaagcagc catttcgcgg cttcgtagtg ttccatatca ccatgataat caaggtgatc
    28081 gcggcttaag ttggtaaaga ccgacgccgc aaatttcaat gccgccacac ggtgctgtac
    28141 cagtccgtgg gaggaaactt ccattgcgca aaaagtcgcg ccctgatcca ccagccccgc
    28201 cagctcatgc tgaacatcga ctgccgaacc ggttgtattt tctgtcggga tcactttccc
    28261 cagcaggccg ttaccaacgg tgcccattac cgcgctggtt tcgccaagca gttggctcca
    28321 ctgcgccagc agctgggtcg tcgtggtttt gccgttggtg cccgttacgc ccacgagacg
    28381 taaattgtca gagggttcat ggtaaaagcg gcccgccagt gcagataaac gctcgttgag
    28441 ctggctgaga tagatgaccg gtacgccgtg catttcacgg atttcaccgt cggtcgcctc
    28501 atctttcgcc tctgcaataa tggcagccac accttgcgct atcgcctgcg ggatatatcg
    28561 acgcccgtcc gcctgatgac ctactacagc tacaaagaga tcgcccgccg cagccacacg
    28621 gctgtcgagt gtcatctctc gcagtgctcg cgaaggtgcg tctggcaccc acggagcaag
    28681 aaggtcgcgc aaattacgat ctgccacctg tcccctcgcc ttgattaatc acaaattcat
    28741 ttttatcgcc cgttgtcagc gcatccggtt cgatgttcat ggtacgcaat acgccgccca
    28801 tgatggcacc aaagaccggc gcggaaacgg cgccgccgta gtatttaccc gcctgcggat
    28861 cgttgataac aacaaccagc gcgaagcgcg gctgactcgc aggcgcaacg cctgcggtat
    28921 aagcaatata tttattgatg tagcgaccgt ccggcccgac ctttttcgcg gtaccggttt
    28981 taatggcgat acgatagcct ttaatcgccg ccttcacgcc gccgccgccc ggtagcgcca
    29041 cgctttccat catatgcacc acggtgcgaa caatggattc cgggaagact cgttcaccgg
    29101 gaaccggggg gtcaactttg gtaatcgaca gtgggcgata aatgccgtag ctgccgatag
    29161 ttgcgtagac tcgcgctaac tgtaacggtg ttaccattag cccgtagccg aaagagaagg
    29221 tggccctctc tatgtcagac caccgttgtt tttgaggata taagccactg cgttctccga
    29281 ccaaccccaa attggtcgct tttcccagtc caaaacgtga gtaagtatct actaacgctg
    29341 aggacggcat cgctaacgcc agcttggaaa caccgacgtt actcgacttc tgtaataccc
    29401 cggtcagggt taattcgctg tagcgtgcca cgtctttgat ttcgtggccg ttaattcgat
    29461 aaggaacggt attcagtacc gagttttccc gcactacgcc acgttgcaac gcggtcatca
    29521 ccaccatcgg tttaaccgtt gaacccggtt caaacacgtc agtgatggta cggttacgca
    29581 tcgcctcttt cggcgtgcca ctcagattgt ttgggttgta tgacgggctg ttagccatcg
    29641 ccagcacttc accggtgttg acatccacca gcacggcgct accagattca gctttgttaa
    29701 aggccaccgc gttgttcagt tcgcgataaa ccagcgcctg caggcgttca tcaatactca
    29761 gcgccaggtt gtgcgccgcc tgactgtcgg tggaagagat atcttcaatt acgcgaccat
    29821 agcggtcttt acgcacaatg cgctcacccg gctgcccggt aagccattta tcgaaactct
    29881 tctcgacgcc ctcaatcccc tgactatcga cgttagtaaa gccgatgagg tgagcggtca
    29941 cttcgccgga tggataataa cggcgagatt cttcacgcag atgaatcccc ggcagtttca
    30001 gttttttgat gtactctgcc atgtcagggt ttacctgacg cgccagataa ataaagcgcc
    30061 ctttcgggtt ggcgttaatg cgggctgaaa gctgatccag cggaatattg agtgcgttag
    30121 ccagcgcctt ccagcgatca ccgacgctga taccgccagc gtcatgcact tctttcgggt
    30181 cagcccaaat cgcttttacc ggcacgctca ccgctaacgg gcgaccagaa cggtcagtaa
    30241 tcatgccgcg ggaggtggaa acttgctgaa cgcgaagaga acgcatgtcg ccctctttta
    30301 ccagcatatc cggggagata acttgtaacc acgctacgcg tccgagcaga aaagccagcg
    30361 ccaggagaat acagccgcat aacaacgcaa aacgccaact gataaagttg gcatgttctt
    30421 cctgacgttt tggtttctgc gttttcgccg ctgctttcat gcgtcgcgtt tatccttatt
    30481 tttgcactac gatattttct tgtgacggat caacatgctg catttgcagc ttttccgtgg
    30541 cgatcctttc cacccggcta tggtcgccga gcgcattctc ttcaaggatc aggttgcgcc
    30601 attcaatgtc taaagcatct cgctccagca ccagttgttc gcgctgtgcg gtcagtaaac
    30661 gggtatggtg cgccgtggtt accacggtca ccgccgtcaa aataatgcaa atgaacaggc
    30721 agagtggcag cttcccaaat cgcaaaagat cgtcaccgat aacaccaggc aatgcatggc
    30781 gctcgtggct tcccatcgat cctttaactt tgcttagagc ttctgtcact ctgctgatca
    30841 tgcgttcgtc ctctctgcaa tacgcagaac tgaactacgg gcacgagggt tctcagccac
    30901 ctcttcttca cccggcatta acttgcctag tgctcgcagc tgacggccac ccagtttttt
    30961 gagctgctct tcagtcatcg gtaaccctgc cggaacttgc ggaccgcggc tgttttcacg
    31021 cataaaacgt ttcacaatac ggtcttccag cgagtggaag ctgatgatcg aaagccgccc
    31081 acccggggcc agcacgttga gcgagctttt tagcgcctgc tctatctcct ccagttcact
    31141 gtttacccaa atgcgcaccg cctggaaggt acgggtcgcg ggatgtttaa acttatcttt
    31201 caccggcgtt gcagcagcca cgacttccgc cagttctttg gtgcgggtca tcggctgttc
    31261 gcggttacgc tcgacaatgg cgcgggcaat gcgtttggca aaacgctctt caccataggt
    31321 tttcaatacc caggcgatat cggcttcttc tgcggtttgt agccattcag cggctgactg
    31381 cccacgggtt gggtccatac gcatgtccag cggaccatcg cgcataaagg aaaagccgcg
    31441 ttcagcatca tcaagttgcg gtgaagagac gccaagatcg aggagaatgc cgtcgatctt
    31501 gccgataaga tcgcgctcgg caacgtattc gcccagcgcg gagaaaggtc cgtggatgat
    31561 ggagaagcgc ggatcatcaa tagtcttcgc aacggcgata gcctgcgggt cgcgatcgat
    31621 cgccagcaaa cgcccctctt cgccaagctg cgagaggatc agacgtgagt gaccaccgcg
    31681 accaaaagtc ccatcaatgt agatgccatc aggacggata ttgaggccat taacggcttc
    31741 atccagcagc accgtagtat gtttatagtt ttccatcatt ttatagagac aagtcctgca
    31801 gtcgctccga taagtctcca gtagccaact gctctgcgtc gatatcttcc ttgacctgtt
    31861 gatgccaggt tgtttcatcc cacagctcaa acttgttgaa ctgtccaacc agcatcactt
    31921 cttttgtcag cccggcatgt tgccgcagta ctggcgcgat taacaatcga cctgcgccat
    31981 ccatctgaca ttcgctggca tgacctaaca gtaggcgctg cacacggcgc tcaaccgggt
    32041 tcatgctcga cagacgcgat aatttttgct cgataatttc ccattcaggc agggggtaaa
    32101 gcagcaggca cgggtgatga atgtcaatgg tgcaaaccat ttgaccggca gcgttctcaa
    32161 gcagctgttc ccgataacgg gtaggcactg ataagcgccc tttgctgtcg agattgacta
    32221 acgttgctcc ccggaacatg ccagcctcac cccttattcc cactttgccc cacaatttcc
    32281 cactgaaagg agtttacgga gcggaggaaa agcttgtcaa gccagtcaca aggcataccg
    32341 aggtaaaaaa cccgatgttt acggctaata tcagcgttgt ttattttacg tccaacgtgc
    32401 gaagcaaatt aacgttatga atagctgtaa gaaaaaatcc ctcatcgtca tcaccgtttt
    32461 aaaatgactc actattttcc acagcaaata taaagtgtca gtttgcgacg cgagcggcat
    32521 tttaggacat atcttccccg gttaacagtc cctgttgcgt ctgtgtggcg cttgatgcca
    32581 gcgggtttgc gcaaaagcaa agaaaagtgt ttgttaaatc ggcaattcat ctctgcatgt
    32641 aacaaaataa tgcaaaaacg cgctatcgat atcacttaat tattgtgaat ttcagctggt
    32701 gaggtaatta acgggcaggt ttaaaagcat ttatcaataa tagccaggaa tagtcttatt
    32761 tactttaagg atattgatgt ccattcccgt actcaacgcg ccagagagaa attcacctgg
    32821 cgcgtatttt tgttcgcggc ttagctacgg ctgagcacgc cgcggcgata gagattacgt
    32881 ttaatgcgcg ttaaaccagg ttttggctta cgcggttcgt ccaggcttgc caggacaatc
    32941 tccagcacac gctctgcgac atcgcggtga cgttgagcca ctgccagcac cgggcactgt
    33001 aagaagtcga gcagttcgtt atcgccaaag gtggcaattg ccaggtcaga aggcagtttg
    33061 ccgtcacgac gtagcgtgac atccatcacc ccttgcaaca acgcaaacga cgttgtgaac
    33121 agtgcctgcg gcatcggatg cgtttccagc catttttcga ataactgggc agccgcctcc
    33181 cgctcatagc tgttggcata aaggaaatgc acttcgcgag gatcatcttt ccaggcagta
    33241 cggaaacctt gttcacgcag gaagctgaca gaaagctccg gcagcgcgcc aagataaagt
    33301 accgtctcgg cgggaaactt acgtaactct tccgccagca tttcggcatc atcctgatcg
    33361 gcaccaacca cgctggtgaa gtgttcacga tcgagggcgc ggtccagcgc gacaatcggg
    33421 aacgggtcgt tagcccagcg ttgataaaaa ggatgctcag gtggcaacga cgtcgaaaca
    33481 ataatggcat caacctgacg ctgtaaaaga tgctcgatac aacgcatttc gttgtcgggc
    33541 tgatcttccg agcaggcaat cagcagttga taaccccttt gccgcgcctg gcgttcaaga
    33601 tagttagcga tgcgggtata gctggtgttc tccagatcgg ggatcacaag accaatagaa
    33661 cgtgtgcgtc cagcacgaag cccagctgcc acggcgttcg ggtggtaatt gtgctcacgc
    33721 accacagcca tgactttttc aacggttttg tcgctcacac ggtattgctt cgctttgccg
    33781 ttaataacat agcttgcagt ggtccgcgac actcccgcca gccgagcgat ttcatccagt
    33841 ttcacaattg ccccttgcgt aaaatgtaaa aaccatagcc attgtactgg tatgggttaa
    33901 attcattaac atctaagcgc agaaaattaa ctgcggcaac tgcttttatt ccaggttccc
    33961 actgatttcg caaaaaaagc ccaacgtgac atgttgggct gcaaattgcg cattgagatc
    34021 attctcaacg cattatttta tcgccgcgcg aaagtccgac tacaccagag cgagcaactt
    34081 ccacaatttt cgccacatcg cgaatcgatg ctaaaaatgc atcaagctta tcgctggtgc
    34141 ctgctaattg aacggtataa agcgagggtg tgacatcgat aatttgccca cggaatattt
    34201 ccgtattacg tttcacttca tcacgcccgt aaccgctggc ctgaattttc accagcatga
    34261 tttcccgctc aacgtgcgcg ccctgcccca actcgctcac gcgcaagaca tcgaccagct
    34321 tgtgtaattg cttttcgatt tgctcaagca ctttttcatc gcccacggtc tggatggtca
    34381 tacgcgataa tgttggatcg tcggttggcg caacggtcag gctttcaatg ttgtagccac
    34441 gctgggaaaa aaggccaatc acgcgggata acgcgcctga ttcattttcg agtaagactg
    34501 ataatatccg gcgcataatc aggttctctc cgttttgctt aaccacattt catccattcc
    34561 gcccccgcga atctgcatcg ggtagacgtg ctcactgcca tcgacggtga catcaacaaa
    34621 caccaggcga ttattgcgca cctgttccag cgcctcgcta agtttgcttt ccagttcttg
    34681 cggatgagag atctggatcc cgacgtgacc ataggcttcc gccagacgaa cgaaatcggg
    34741 tagtgactgc atataagatt gtgaatgacg gccggaatag atcatgtcct gccactgttt
    34801 caccatcccc agatagcggt tattgagatt caccaccagt accggcaact cgtattgcaa
    34861 cgctgtagac agttcctgga tgttcatctg aatactgccg tcgccagtga cgcaaaccac
    34921 ggtttcttct ggcaacgcca ttttgacgcc cagtgccgca ggtaaaccaa aacccatcgt
    34981 gccgaggccg ccagagttaa tccagcgacg cggtttgtcg aatggataat aaagcgcagc
    35041 aaacatctgg tgctgcccga catcggacgt cacgtaagcg tccccattag tcaaccgcca
    35101 aagagtctcg atcaccgcct gcggtttaat cttttcactg tgagtgtcat atttcaggca
    35161 ctgacgagcg cgccactgtt caatctgctg ccaccagtcg cggatctcat ccagcggttg
    35221 atggacggat tcttgcgaca ggagttcaag catttgttcg aggacctggc gagcatcccc
    35281 cacaatcggg atatcagcag tcacggtttt agaaatggaa gtaggatcaa tatcgatatg
    35341 cagaaccgtg gcatttgggc agtacttcgc cagattgttc gtcgttcggt catcaaaacg
    35401 taccccgacg gcaaaaatca catccgcgtt atgcatcgtc atattggctt cgtaggtacc
    35461 gtgcattccc aacatgccca gtgcctgacg atgcgttgcc ggaaacgccc ccagccccat
    35521 taatgaggaa acaacgggca gattcaacgc ctccaccgtt tcttttagct gctgatgaca
    35581 gcccgccgtg attgccccac cgcctacata gacaaccggt ttttttgccg ccaccagcgt
    35641 ttgcagagcg cgcttaattt gccctttatg cccggaggtc gtgggattgt aagaacgcat
    35701 actgaccgac tccggccaga catagggtaa tttgttcgcc ggatttagaa tatctttcgg
    35761 taaatcaacg accacgggtc caggacgacc acttgccgcc agccagaaag cctttttcag
    35821 cacctgcgga atgtcttccg tttgcttaac cagaaaactg tgtttaacca ccgggcgaga
    35881 aatccccacc atgtcgcact cctgaaaggc atcgtagcct atcaacgagg tcgctacctg
    35941 cccggaaagg acaactaagg gaatggaatc catataagcg gtggcaatgc cagtaatcgc
    36001 attggtcgcc cctggacccg acgttaccag cacgacgccg acttccccgg tcgcgcgcgc
    36061 cagaccatcg gccatatgca ccgccgcctg ctcatgacga actaaaacat gatcgatacc
    36121 acccacggta tgcaatgcat cgtaaatatc aaggaccgcg cctccgggat aaccgaatac
    36181 ttgtttaacg ccctgatcga taagcgatcg gacgaccatc tcggctccag acaacatctc
    36241 catggcctgc ctcactgttt gacggaaaaa atgtgtaaaa cacattaacc gtccgatctt
    36301 gcttaagcaa ttggcagcct gagacataaa cacggggtaa taagaataaa caggaggaaa
    36361 ggtgaaaaga aacgataaaa tcctccatta cagaggatta aattgataat aaatcggc
//
LOCUS       DAZGQL010000051        35957 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.051, whole genome shotgun sequence.
ACCESSION   DAZGQL010000051 DAZGQL010000000
VERSION     DAZGQL010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35957
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.051"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            151..741
                     /locus_tag="ACHKIR_002737"
     CDS             151..741
                     /locus_tag="ACHKIR_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO9990451.1"
                     /translation="MSKKLGFALSGIMLAMAAGTAFAANDMDGGQLNISGLVVDNTCE
                     ARVDGGNKDGLILLQTATVAEITDGVLDTTVGAKAKPFSITIDCSKANPAPGTTAKMT
                     FGSVFFSNSKGTLNNDMSINNPSDGVNIALHNIEGSTIKQVQVNNPGDVYSKTLDATS
                     KTATYDFKASYVRADASKAATAGYVKTNSAYTITYQ"
     gene            850..1590
                     /gene="yadV"
                     /locus_tag="ACHKIR_002738"
     CDS             850..1590
                     /gene="yadV"
                     /locus_tag="ACHKIR_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFO9990452.1"
                     /translation="MFFNTKHTAALCLASCMAFSSSAIADIVISGTRVVYKSEQKSVN
                     VRLENKGNNPLLVQSWLDTGDDNAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTASS
                     ALPKDRESVFWFNVLEVPPKPDAAKAANQSLLQLAFRTRIKLFYRPEGLSGIPSDAPL
                     ALKWSWATSEGKAALRVDNPTPYYVSFSSGDLEANGKRYPLDVKMIAPFSNDVIKVNG
                     MSGRTSSAKVHFFAINDFGGSIEGNANL"
     gene            1625..4225
                     /gene="htrE"
                     /locus_tag="ACHKIR_002739"
     CDS             1625..4225
                     /gene="htrE"
                     /locus_tag="ACHKIR_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein"
                     /protein_id="HFO9990453.1"
                     /translation="MTIKSTKKYHNHLTRIATFCALLYSNSALCAELVEYDHTFLMGK
                     DASNIDLSRYTEGNPTLPGIYDVSVYVNDQPIMSQSIAFTVIEGKKNAQACITQKNLL
                     QFHISSPDKNSEKAILLKRDEDLGDCLNLAEMIPQSSIRYDVNDQRLDIDVPQAWIMK
                     NYQNYVDPSLWENGINAAMLSYNLNGYHSESPGRTNDSIYAAFNGGINLGAWRLRASG
                     NYNWMTNVHSDYDFQNRYLQRDLASLRSQLVIGESYTTGETFDSVSIRGIRLYSDSRM
                     LPPVLASFAPIIHGVANTNAKVTVMQNGYKIYETTVPPGAFAIDDLSPSGYGSDLIVT
                     IEEADGTKRTFSQPFSSVVQMLRPGVGRWDISAGQVLKDSIQDEPNLFQASYYYGLNN
                     YLTGYTGIQLTDNNYTAGLLGLGMNTPVGAFSVDVTHSNVSIPDDKTYQGQSYRISWN
                     KLFENTSTSLNIAAYRYSTQHYLGLNDALTLIDEVEHPEQDLEPKSMRNYSRMKNQVT
                     VSINQPLKFEKKDYGSFYLSGSWSDYWASGQNSTNYSIGYSNSASWGSYSISAQRSLN
                     EDGQTDDSIYLSFTIPIENLLGTEHRSSGFQSIDTQLNSDFKGNNQLNISSSGYSDTN
                     RISYSVNTGYMMNKSSDDLSYIGGYASYESPWGTLSGSASASSDNSRQFSLNTDGGFV
                     LHSGGLTFSNDSFSDSDTLAVIQAPGAKGARINYGNSTVDRWGYGVTSALSPYHENRI
                     ALDINDLENDVELKSTSTVAVPRQGAVVFADFETVQGQSAIMNIVRSDGKNIPFAADI
                     YDEQNNIIGNVGQGGQAFVRGIGQEGNIRITWIEEGKPVSCFAHYQQNTTSEKIAQSI
                     ILNGLRCQIQ"
     gene            4242..4814
                     /locus_tag="ACHKIR_002740"
     CDS             4242..4814
                     /locus_tag="ACHKIR_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO9990454.1"
                     /translation="MIKTTAHKIAILMGLLLSPSVFATDVNVDFTATVKATTCNITLT
                     SLNGSNVVDNGNDSYTLIIPKMGLDKIVNKASQSEANFKLVASGCSSGISWIDTTMTG
                     NQSGSTSLIIPQSSDSSSTTSNIGMGFKRLETSGDTFLKPNSAEYIRWKTTEISTSGL
                     EMTVALRETTPGQGIPGKFRAQATFNFIYQ"
     gene            4826..5434
                     /gene="yadL"
                     /locus_tag="ACHKIR_002741"
     CDS             4826..5434
                     /gene="yadL"
                     /locus_tag="ACHKIR_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020235143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein YadL"
                     /protein_id="HFO9990455.1"
                     /translation="MMILKKLCYGLSGSLFLASSLLTISSHAATDSIGLTVTTDIEMG
                     TCTSTLTDDGTAKLSTINFGDVYISEINAETKIKTFKLQFKDCAGIPGKKAKIKLTPR
                     ALCEGNSNNGPGFANASTATAKAAAVAVEVWSTSTPGKNGAKQFSCVTPATEEVSIAG
                     ATGGDVVDYPMSAVLVVAKDKTVTDVTAGDFTAPATFTVTYN"
     gene            5461..6057
                     /locus_tag="ACHKIR_002742"
     CDS             5461..6057
                     /locus_tag="ACHKIR_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HFO9990456.1"
                     /translation="MRPTQRNLMKKSILLLSLLFSTTFPVVAGQDVDIVANIKNNTCQ
                     SGISNNGNIDLGVVGVGDFTGNISAENYHPGGKEFTITVQDCTLQGTGNVLNQLHIDF
                     RALSGVMATGSSQIFANEDNTGAKNVGVVIFSIQDPANTFNVLGVTGSSRSVYPVMSS
                     ALNNSSWKFSTRMQKIDPALNVTSGQLISHVLVDIYYE"
     gene            6083..7372
                     /locus_tag="ACHKIR_002743"
     CDS             6083..7372
                     /locus_tag="ACHKIR_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like adhesin"
                     /protein_id="HFO9990457.1"
                     /translation="MQHDNGVKMKIFFRYFLFLALCSCCYTASAGTDDNVSYLVGNDY
                     GVGPGDQKWRETGPSADITVKFRYSTSTNNLVFYKPTQLGPTGVSLYWSQLNSASGGG
                     YLYCNRSNTASGEPMAIENQMVDSGKRYGGHKLFETSVPGLYYTLKISNIWSSYTTTN
                     ISSSGMYIGDSPTQYFRWIPESEATLYWSCNNANSSNKYWAVGGVWQSLTVEFYTDTN
                     FDPTKTNKVTLSKPGGYLYSFKGYGLGGGINDHSYFLKIDFDLDNMTLSNPTCFTAAL
                     TGPSVSGSTVKMGDYTPAQIKNGATAVPFDITLQNCIRVRNIETKLMSNKVGSVSKEL
                     LANTLTGNNAAKGVGVLIEGLKNTKSAQMVLKPNDSTSVYKDYETEDDTTGGIYPDKG
                     NGTSQPLHFQATLKQDGNIAIEPGDFKATSTFQVTYP"
     gene            7484..8278
                     /gene="panB"
                     /locus_tag="ACHKIR_002744"
     CDS             7484..8278
                     /gene="panB"
                     /locus_tag="ACHKIR_002744"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFO9990458.1"
                     /translation="MKPTTIASLQKCKQDKKRFATITAYDYSFAKLFAEEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVQMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAG
                     DQLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            8290..9141
                     /gene="panC"
                     /locus_tag="ACHKIR_002745"
     CDS             8290..9141
                     /gene="panC"
                     /locus_tag="ACHKIR_002745"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFO9990459.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSENSKRAVILVAAWLGDARLID
                     NKIVELA"
     gene            complement(9223..9438)
                     /locus_tag="ACHKIR_002746"
     CDS             complement(9223..9438)
                     /locus_tag="ACHKIR_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000404120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO9990460.1"
                     /translation="MEIKTRRETRQTLAQWFEEKGFQKGFQKGYKEGLQKVRLAQRLL
                     SKGMSREDVAEVTALSLAEIDKLINSN"
     gene            complement(9507..10451)
                     /locus_tag="ACHKIR_002747"
     CDS             complement(9507..10451)
                     /locus_tag="ACHKIR_002747"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004536 - DNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFO9990461.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLDIHLPAELRELCDLDTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMTFRMMRYAIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNNPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGESMMTLAQWFEEKGRQEERQEVRQEVRQEVRQEVIQEV
                     RQEVRQEYALRFLSKGMSREDVAEMANLPLAEVDKLIN"
     gene            10725..11105
                     /gene="panD"
                     /locus_tag="ACHKIR_002748"
     CDS             10725..11105
                     /gene="panD"
                     /locus_tag="ACHKIR_002748"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFO9990462.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(11109..12338)
                     /gene="yadE"
                     /locus_tag="ACHKIR_002749"
     CDS             complement(11109..12338)
                     /gene="yadE"
                     /locus_tag="ACHKIR_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFO9990463.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLVTWKDTPVYNAPSAGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYTTLSMVQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFIVTSRIKRHPQKW
                     DPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAAKEAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(12402..12842)
                     /gene="yadI"
                     /locus_tag="ACHKIR_002750"
     CDS             complement(12402..12842)
                     /gene="yadI"
                     /locus_tag="ACHKIR_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFO9990464.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMLV
                     YRETLSSAEFRERIVELGAPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(12947..13717)
                     /gene="yadH"
                     /locus_tag="ACHKIR_002751"
     CDS             complement(12947..13717)
                     /gene="yadH"
                     /locus_tag="ACHKIR_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO9990465.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(13714..14640)
                     /gene="yadG"
                     /locus_tag="ACHKIR_002752"
     CDS             complement(13714..14640)
                     /gene="yadG"
                     /locus_tag="ACHKIR_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO9990466.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            14749..15411
                     /gene="can"
                     /locus_tag="ACHKIR_002753"
     CDS             14749..15411
                     /gene="can"
                     /locus_tag="ACHKIR_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HFO9990467.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(15452..15988)
                     /gene="hpt"
                     /locus_tag="ACHKIR_002754"
     CDS             complement(15452..15988)
                     /gene="hpt"
                     /locus_tag="ACHKIR_002754"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFO9990468.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            16194..18584
                     /gene="gcd"
                     /locus_tag="ACHKIR_002755"
     CDS             16194..18584
                     /gene="gcd"
                     /locus_tag="ACHKIR_002755"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HFO9990469.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILAWAGFNDPQEISGTLSADTTPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHKLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKNLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(18780..20330)
                     /gene="cueO"
                     /locus_tag="ACHKIR_002756"
     CDS             complement(18780..20330)
                     /gene="cueO"
                     /locus_tag="ACHKIR_002756"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HFO9990470.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGGKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLTEETTLHW
                     HGLEVPGEVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFNADGQIDYQLDVMTAAVGWFGDTLLTNG
                     VIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVNELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPATHRAGWKDTVKVEGNVSEVLVKFNHDAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            20496..20843
                     /gene="yacC"
                     /locus_tag="ACHKIR_002757"
     CDS             20496..20843
                     /gene="yacC"
                     /locus_tag="ACHKIR_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HFO9990471.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            20949..21815
                     /gene="speE"
                     /locus_tag="ACHKIR_002758"
     CDS             20949..21815
                     /gene="speE"
                     /locus_tag="ACHKIR_002758"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HFO9990472.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAVHTAAFALP
                     QYLQDALASQPS"
     gene            21831..22625
                     /gene="speD"
                     /locus_tag="ACHKIR_002759"
     CDS             21831..22625
                     /gene="speD"
                     /locus_tag="ACHKIR_002759"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HFO9990473.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTTEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITTALWKEMREIYYGRNMPAV"
     gene            complement(22663..23025)
                     /gene="yacL"
                     /locus_tag="ACHKIR_002760"
     CDS             complement(22663..23025)
                     /gene="yacL"
                     /locus_tag="ACHKIR_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YacL"
                     /protein_id="HFO9990474.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(23200..25797)
                     /gene="acnB"
                     /locus_tag="ACHKIR_002761"
     CDS             complement(23200..25797)
                     /gene="acnB"
                     /locus_tag="ACHKIR_002761"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019080489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HFO9990475.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFDQLSQYTEKA
                     DGVIFQTAV"
     gene            26152..27909
                     /gene="yacH"
                     /locus_tag="ACHKIR_002762"
     CDS             26152..27909
                     /gene="yacH"
                     /locus_tag="ACHKIR_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HFO9990476.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASTGLYIKSRTVEAPVEPQSTQ
                     LAAPDISAVTLPATVSVPPVTPAVVKSAFSTAQIDQWVAPVALYPDSLLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQEVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKAVPVKQTVTAPVIPSN
                     TVSTANPVITEPATTVISIEPTNPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FVDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDGNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHSAPVITRDTQRQAAAQRFNEAENYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVREKMQTNPQIQQRREAARERIQSATPEQRQ
                     VFKEKVQQRPLNQQQRDNARQRVQSASPEQRQVFREKVQESRPQRLNDSDHTVRLNNE
                     QRSAVRERLSERGARRLER"
     gene            complement(27980..29404)
                     /gene="lpdA"
                     /locus_tag="ACHKIR_002763"
     CDS             complement(27980..29404)
                     /gene="lpdA"
                     /locus_tag="ACHKIR_002763"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFO9990477.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(29729..31621)
                     /gene="aceF"
                     /locus_tag="ACHKIR_002764"
     CDS             complement(29729..31621)
                     /gene="aceF"
                     /locus_tag="ACHKIR_002764"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFO9990478.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAASAPAAGVKDVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(31636..34299)
                     /gene="aceE"
                     /locus_tag="ACHKIR_002765"
     CDS             complement(31636..34299)
                     /gene="aceE"
                     /locus_tag="ACHKIR_002765"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HFO9990479.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(34460..35224)
                     /gene="pdhR"
                     /locus_tag="ACHKIR_002766"
     CDS             complement(34460..35224)
                     /gene="pdhR"
                     /locus_tag="ACHKIR_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HFO9990480.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            complement(35680..>35957)
                     /locus_tag="ACHKIR_002767"
     CDS             complement(35680..>35957)
                     /locus_tag="ACHKIR_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFO9990481.1"
                     /translation="DKVEDYTEREFIELLGEFTNPTGDNAQLKGEELDKYWDDLEEHL
                     TRITQHPLMSDLIYYPAKKGDDKPENILKIVKEWRRSQGLSLFKDSE"
BASE COUNT         9088 a         9367 c         8576 g         8926 t
ORIGIN      
        1 agccatcccg atgatgaatg aatgtaatag ttggtaaaca acagacaaac atcattaaca
       61 cagtaacgat tttgttacgc agcattgcag cagactctta tcgtcatgcg gtgcggttat
      121 ctctctatgg aatattattc aaggattttt atgtctaaaa aattaggttt tgcgctgtct
      181 ggtattatgt tggcaatggc tgctggtact gcatttgctg ctaacgatat ggacggtggt
      241 cagttaaata ttagcggcct ggtcgttgat aacacctgtg aagctcgtgt tgatggcggt
      301 aacaaagatg gtttgatcct gctgcaaacc gctactgttg ctgaaatcac cgatggtgta
      361 ctggatacca ccgtaggtgc taaagctaag ccattcagca ttactattga ctgcagcaaa
      421 gctaacccgg ctccgggtac tactgcaaaa atgactttcg gttccgtgtt ctttagcaat
      481 agcaaaggta ctctgaataa cgatatgtct atcaataatc catctgatgg tgtgaatatt
      541 gccctgcata atattgaagg ttccactatt aagcaggttc aggttaataa cccgggtgat
      601 gtttacagca aaactctgga tgcaacgtct aaaactgcaa cctacgattt taaagcatcc
      661 tatgttcgtg ctgatgcaag caaagcagca actgccggtt atgtaaaaac taactcagca
      721 tataccatta cttaccagta aatatcagcc acctaaattg attgggtgaa tatatatagg
      781 gaataacatc aaacatattt atttttgaac gttattccct gttttaatta actctgtgag
      841 gaacggttta tgttttttaa tactaaacat accgcagcat tgtgcttagc gagctgtatg
      901 gcttttagct catcagctat tgcagacatc gtaatttcag gtacacgcgt agtttataaa
      961 agcgaacaaa aaagcgtcaa tgtgcgtctg gaaaataaag gcaataaccc attgctggtt
     1021 cagagttggt tagatactgg agatgacaac gctgaaccag gcagtattac tgtccccttt
     1081 accgctacgc caccagtatc acgtattgat gctaaacgtg gccaaacaat caaactgatg
     1141 tacaccgcca gtagtgctct gccaaaagac agagaaagtg tgttctggtt taacgtactg
     1201 gaagttccac caaaacccga tgcagcaaaa gcagcaaatc aaagcctgct acaactggca
     1261 tttcgtacac gtataaaact gttctatcgc ccggaaggat tgagtggaat tccatctgat
     1321 gcgccattgg ctctgaagtg gtcctgggca acatctgaag gcaaagcggc attacgcgtt
     1381 gataacccta cgccctacta tgtctctttt agcagtggtg atttagaagc caacggaaaa
     1441 cgctatcccc tggatgtgaa aatgattgca ccgttcagta acgacgtcat caaggtaaat
     1501 gggatgagtg gcagaacaag ctctgcaaaa gtgcattttt tcgccattaa tgattttggc
     1561 ggctctattg aaggcaacgc caacctttaa tctggcaagg atataccaca gcaggaagca
     1621 gaacgtgact attaaatcca ctaaaaaata tcataatcac cttacccgca tcgccacgtt
     1681 ttgcgcgctg ctgtatagca acagtgcttt atgcgctgaa ctcgtcgaat atgaccatac
     1741 atttctgatg ggaaaggatg cttcaaatat cgaccttagt cggtatacag aaggcaaccc
     1801 gacactacca ggcatttacg atgtcagtgt gtatgttaac gatcaaccta tcatgagcca
     1861 aagcattgct tttactgtta ttgaaggtaa aaagaacgct caggcctgca ttacgcagaa
     1921 aaatttattg caatttcata tcagttcacc agacaagaat agcgaaaaag ccattttgct
     1981 taagcgagac gaagatcttg gtgattgcct taatctggct gaaatgatcc ctcaatcatc
     2041 cattcgttac gatgttaacg atcaacgcct ggatatcgac gtcccccagg catggattat
     2101 gaagaattat caaaactatg ttgatccctc tttgtgggaa aatggcatta atgctgccat
     2161 gctttcctac aacctcaacg gatatcacag tgaaagtcca ggcagaacaa atgacagtat
     2221 ctacgcggca ttcaacggtg ggataaactt aggtgcctgg cggttacgtg cctcgggtaa
     2281 ctacaactgg atgaccaatg tccatagcga ttacgatttt cagaatcgct atttacaacg
     2341 cgatcttgcg tcactacgtt cacaactggt tattggtgaa tcttacacaa ccggcgaaac
     2401 cttcgattca gtcagtattc gcggtattcg cttatacagt gacagtcgaa tgctgcctcc
     2461 ggtattagcc agttttgcgc caatcattca tggcgtagcg aataccaatg ccaaagtaac
     2521 cgttatgcaa aatggttata aaatctatga aacgacggtg ccgccagggg cctttgccat
     2581 tgatgatctt agcccgtcag gttatggcag tgatctcatt gtgactatcg aagaagctga
     2641 tggcacaaaa cggacgttct cccagccatt ttcatctgtt gtgcaaatgc tgcgccctgg
     2701 cgttggtcgc tgggatatta gcgccggtca ggttttaaaa gacagtatcc aggatgagcc
     2761 taatttattt caagccagct actactatgg tttgaataac tacctgacag gatataccgg
     2821 tattcagcta actgataata actataccgc cggattgctg gggcttggta tgaatacccc
     2881 cgtgggggct ttttctgtcg atgttactca ctcaaatgtc agcattccgg acgataaaac
     2941 ctatcaagga caaagctacc gtatttcctg gaacaagtta tttgaaaata ccagtacatc
     3001 attaaatatc gcggcctatc gctactccac ccagcattat ctgggcctca atgatgcgct
     3061 gacgctgatt gatgaagtag aacatcctga gcaagatctt gagcctaaat ccatgcgcaa
     3121 ttactcgcgc atgaaaaatc aggtcacggt cagtatcaac caaccgctga aatttgagaa
     3181 aaaggattac ggttcatttt atctctcggg aagctggtca gattactggg cctccgggca
     3241 aaacagtact aattactcta ttggttatag caatagtgcg tcctggggca gttacagtat
     3301 cagtgctcaa cgttcgttga atgaagatgg tcaaaccgac gatagcattt atctcagctt
     3361 cacgattcca atagaaaatt tactgggtac ggaacaccgc agctccggct tccagagtat
     3421 tgatacacaa ttaaacagcg actttaaggg taataaccaa ctcaatatca gcagtagtgg
     3481 ttacagcgat accaatcgca tcagctacag cgtaaacacg ggttatatga tgaataaatc
     3541 cagtgatgac ctgagttata tcggcggtta tgccagttat gaatcgccgt ggggaactct
     3601 gtcaggttca gcctcagcaa gcagtgataa tagtcgccaa ttctcactca atactgatgg
     3661 tggatttgtg ttacatagcg gcgggctgac gttcagtaat gacagtttta gtgattccga
     3721 cactctggct gtcattcagg cgccaggagc aaaaggcgct cgtattaact acggaaacag
     3781 caccgtagac cgctggggtt atggtgtgac cagcgccctt tcgccatacc atgaaaaccg
     3841 tatcgcactg gacattaacg atctggaaaa cgatgttgaa ttgaaaagca ccagtactgt
     3901 cgccgtcccg cgccagggag ccgtcgtctt tgctgatttt gaaacagttc agggacaatc
     3961 ggcaattatg aatatcgtgc gaagcgatgg taaaaatatt ccatttgcag cggatattta
     4021 tgatgaacaa aacaatatca tcggcaacgt aggtcagggt ggtcaggcct ttgttcgcgg
     4081 tatcgggcag gaaggaaata tacgaattac ctggattgaa gagggcaaac cagtcagttg
     4141 ttttgctcat taccaacaaa acacgacatc agaaaaaata gcgcaatcta ttattctgaa
     4201 cggacttcgt tgtcagatac agtaaccaca agggacttcc aatgataaaa accacagcac
     4261 ataaaatagc aatactcatg gggctattat tatcgccatc tgtatttgca acggatgtta
     4321 atgtcgattt taccgctacc gtgaaagcga caacatgtaa catcaccctg acatcgctga
     4381 atggttcaaa cgtcgttgat aatggcaatg atagttatac cctgataatc cccaagatgg
     4441 gactggataa gatagtcaat aaagcgtcac aatctgaggc caacttcaaa ttagtcgcca
     4501 gtgggtgtag cagtggaatt agctggattg ataccacaat gactggaaat cagtctggta
     4561 gtacatcgct gattattcca caatcttctg actcctcctc aacaaccagt aatattggta
     4621 tgggatttaa aagattagag acttcgggcg atacgtttct gaaacccaat agcgcggaat
     4681 acatccgctg gaaaaccaca gaaattagca ccagtggtct ggaaatgact gtcgcactgc
     4741 gcgaaacaac cccaggacag ggcatacccg ggaaatttcg cgcacaggca acgttcaatt
     4801 tcatctatca ataacaagga tgacaatgat gatattaaaa aaattatgtt atggattatc
     4861 gggtagtcta tttctggctt catcactgtt aaccatttcc tcacatgcgg ccactgatag
     4921 tattggcctg actgttacta cagacataga aatgggtacc tgtacatcaa cgttgactga
     4981 tgacggtaca gctaaactgt ccaccatcaa tttcggtgat gtatatattt ctgaaattaa
     5041 tgccgaaact aaaattaaaa ccttcaaatt gcagttcaaa gactgtgcgg ggattcccgg
     5101 gaaaaaggcc aaaataaagt tgaccccacg agccttatgc gaaggtaatt ctaataacgg
     5161 ccctggattt gctaacgcat caacggcaac agcaaaagca gcggcagtcg ccgttgaggt
     5221 ctggagtacg tccacaccag gtaaaaacgg cgcaaaacaa ttcagttgcg tcacccccgc
     5281 aacagaagaa gtcagtattg ctggtgcgac gggaggtgat gttgtcgatt acccgatgag
     5341 tgctgttttg gtggtcgcga aagataaaac cgttacagat gtcaccgcgg gtgatttcac
     5401 cgctccggca acatttactg tgacttataa ctaacaggca gaccgattca ggaactaaat
     5461 atgcgcccca ctcaacgtaa tctgatgaaa aaatcaattc tgcttctgtc tttgctattc
     5521 agcaccactt tccctgtggt tgcaggacag gatgttgaca ttgttgcaaa tataaaaaat
     5581 aacacctgcc aaagtggaat cagcaacaac ggaaatattg atttaggtgt cgttggcgtg
     5641 ggcgatttta ccggaaacat ttcagccgaa aattatcacc ccggtggaaa agagttcact
     5701 attacagtac aggactgcac acttcagggc acagggaatg tgttaaatca gttacatatt
     5761 gattttcgcg cactcagtgg cgtaatggcg acaggttcca gtcaaatatt cgccaatgaa
     5821 gacaacaccg gggctaaaaa tgttggggta gttatattct ctattcagga cccggcaaat
     5881 accttcaacg ttcttggcgt aactggtagc tcacgttccg tttatccggt catgtcatca
     5941 gcactaaaca attcatcctg gaaatttagc actcgaatgc aaaaaatcga tccagcgctg
     6001 aatgtcacct ctggccaact tataagccat gtgttagtgg atatttacta cgaataacag
     6061 acaaagtgct gcaagcatta tcttgcagca tgacaatgga gtaaaaatga agatattctt
     6121 cagatacttt ctttttttag cactatgctc ttgttgttat acagcaagtg ccggcaccga
     6181 tgataacgtc agctatcttg ttgggaatga ttacggagtg ggtccgggag accaaaagtg
     6241 gagagaaaca ggccccagtg cagacattac tgtcaaattc aggtattcaa caagcacaaa
     6301 taacttagtt ttttataaac caacccaact cggaccaaca ggtgtgtcat tatactggtc
     6361 tcagttaaat agcgcttcag gtggaggtta cctttactgc aacagaagta acactgcaag
     6421 cggtgagcct atggcaattg aaaatcaaat ggttgattcg ggtaaacgtt atggcgggca
     6481 taaactattt gaaacatctg tcccaggcct gtattataca ttaaagatat caaacatttg
     6541 gtcatcatac accacaacca atatcagttc atcaggtatg tatatcggcg attctccaac
     6601 tcaatatttt cggtggatcc ccgaaagtga agccactcta tattggtctt gtaacaatgc
     6661 aaactcatct aataaatact gggccgtagg tggagtttgg cagtcgctca cggtggaatt
     6721 ttataccgat accaactttg accccacaaa gacaaacaag gtaacattat ctaagccggg
     6781 tggttatctc tactcattca aaggatatgg tcttggtggc ggtatcaacg atcacagcta
     6841 tttccttaaa atagattttg acctggataa tatgacgtta tccaacccaa cgtgttttac
     6901 tgctgcactt actggcccat ctgtaagcgg ttcaacagtt aaaatgggag attatacccc
     6961 agcgcaaata aaaaatggcg caacagcggt tccttttgat atcacgctac agaactgtat
     7021 tcgtgttcgc aatatagaaa caaaacttat gtcaaacaag gtgggcagtg taagcaaaga
     7081 acttctggct aacacactca caggaaataa cgcagcaaaa ggtgttggtg tactgattga
     7141 aggtttaaaa aacactaaaa gtgcacaaat ggtactgaaa ccaaatgatt caacgtctgt
     7201 ttataaagat tacgaaacag aagatgacac taccggagga atttacccgg ataaaggaaa
     7261 cggtacctca caacctcttc atttccaggc tacattaaaa caagacggta atattgctat
     7321 agaaccgggg gattttaaag ccaccagcac ttttcaggtt acctacccct gataacacta
     7381 ttgcaccgcc agacaatggc ggtgcgatac ggataaatca ttgcccccac caatgtgact
     7441 gataaaatgc gagacagcac aacatcattt atcaggatac gctatgaaac caaccacgat
     7501 tgcttcattg cagaaatgca agcaggacaa aaaacgtttc gcaaccatca ccgcttacga
     7561 ctatagcttc gcaaaattgt ttgccgaaga aggacttaac gtcatgctgg taggcgattc
     7621 actgggcatg acggttcagg ggcatgactc cacgctaccg gttaccgttg ccgatatcgc
     7681 ctatcacacc gccgctgtgc gtcgcggcgc gccaaactgc cttctgctgg ctgacctgcc
     7741 atttatggcg tatgccacgc cggaacaagc ctttgaaaac gccgcaacgg taatgcgtgc
     7801 cggagccaac atggtcaaaa ttgaaggcgg tgagtggctg gtggaaaccg tacaaatgct
     7861 gaccgaacgt gccgttccag tttgtggtca tttaggccta accccacagt cagtgaatat
     7921 tttcggtggc tacaaagtcc aggggcgcgg cgatgaagcg ggcgatcaac tgctcagcga
     7981 tgcattggca ttagaagctg ctggggcaca gctgctggtg ctggaatgcg tgccggttga
     8041 actggcaaaa cgtattaccg aagcactggc gatcccggtt attggcattg gcgcaggcaa
     8101 cgtcactgac gggcagatcc tcgtgatgca cgacgcgttc ggcattaccg gcggccacat
     8161 tcctaaattc gctaaaaatt tcctcgccga aacgggcgac atccgcgcgg ctgtgcggca
     8221 gtatatggct gaagtggagt ccggcgttta cccgggcgaa gaacacagtt tccattaagg
     8281 agtgacgttg tgttaattat cgaaaccctg ccgctgctgc gtcagcaaat tcgccgcctg
     8341 cgtatggaag gtaagcgcgt ggcgctggtg cccaccatgg gtaacctgca cgatggccat
     8401 atgaagctgg tcgacgaagc caaagcccgc gccgatgtgg tagtggtcag tattttcgtt
     8461 aacccgatgc agtttgaccg cccggaagat ctggcacgtt atccacggac cttgcaagag
     8521 gactgcgaaa agctaaacaa acgtaaagtg gatttagttt tcgccccttc ggtaaaagag
     8581 atctacccga acggtacaga aacccacacc tatgttgatg ttcctggtct ttccaccatg
     8641 ctggaaggtg ccagccgtcc gggacatttt cgcggcgttt cgacgattgt cagcaagctg
     8701 ttcaacctgg tccagccgga catcgcctgc ttcggtgaaa aagattttca gcaactggcg
     8761 ctgatccgca aaatggttgc tgatatgggc tttgatattg agattgtcgg tgtgccaatt
     8821 atgcgcgcca aagacggtct ggcgctgagt tcccgtaacg gttatctgac ggcggaacaa
     8881 cgcaaaattg cgcccggtct gtacaaagtt ttaagttcga ttgccgacaa attgcaggct
     8941 ggcgaacggg atctcgatga aattattact attgccgggc aagaactgaa tgaaaaaggc
     9001 ttccgcgccg atgatattca gattcgtgat gccgacacct tgctggaggt ctctgaaaac
     9061 agcaaacgtg cagtgattct ggtagccgcc tggcttggcg atgcccgcct gatcgacaac
     9121 aaaatcgtcg aactggcgta atccttatct ggcgctacgg ctgatggcgc cagttattca
     9181 ttgccacgtt atctgtaggc aattattcat atcgtagaca acttagttag agttaattag
     9241 cttatcaatc tctgcaagag ataaagctgt cacctctgca acgtcttccc gagacattcc
     9301 tttactcaga agacgctggg ccagtcttac cttctggagt ccctctttat atcccttctg
     9361 aaatcccttc tgaaatccct tctcttcaaa ccactgcgcc aacgtctgcc tggtttctct
     9421 ccgtgtcttt atttccatat gttcccttaa ttatctggtc agttaacaat aacaattatc
     9481 tggctataac tgataatgca gataacttag ttaatcagct tatcaacctc agcaagaggt
     9541 aaatttgcca tctctgcaac gtcttcccga gacatccctt tactcagaaa acgcagggcg
     9601 tattcctgtc ttacttcctg tctaacctct tgtattacct cctgtcttac ctcctgtctt
     9661 acctcctgcc ttacctcctg cctttcctcc tgtcttccct tctcttcaaa ccactgcgcc
     9721 agcgtcatca tagactcccc tcccgtttcc ctgtctctca gcacaccata aaacaaatcc
     9781 gcttgttcag tatgaccgcg ttgcagcata tagttttgca tggcaactaa ctgacttccg
     9841 ctagtgtacc cttcgtcaat cagcgtgacc agttgctcca gcaataacat taagtcgcgc
     9901 tggcgaatat gtttttgcag tagttcgaga atcgcaatcc gccgatgttg catgatttcg
     9961 tcatccggtg tgatggtgat atccaccagc gggaaagggt tgttgtaaac gcgtcgcgcc
    10021 agctccggcg agtaaaacat atcaaaccag cacattgaga gcggataagg tgtggcctcg
    10081 ccctgataaa acagaatcgg caccaccagc ggcagcttat cgtgatccgc ctccagatgc
    10141 cggtgcatgg cggcaatggc atagcgcatc atgcgaaagg tcattttctt atccggcttg
    10201 ctttgatgtt caatcaccac atgcaaataa ccgggattac cctgcatttg cacggaataa
    10261 agcacgtcag tgctgtgccc tttcaggctt tcttcaataa aactccccga ctcaagatgc
    10321 agcgtgtcga ggtcacacag ttcgcgtagt tccgctggca aatggatatc cagaaagtca
    10381 cgagccgttt ccgcatgcat taaaaattgt ttaaataccg cgtcatgcgg tgtggtactc
    10441 ggtgcatcca tcttccctgc cttccgtgtt gtcttgcgtg aaggcagtat ccagtcaagg
    10501 cgcgccgtcg tcagccgcct ctttattgtt taccgagcag cgttcagaga aatttcaatt
    10561 ggttacaggc gttacatggc acgcttcggc acggtacgca aactgctgat gcaaatgccg
    10621 tcagatgagt agacactaaa caaaaatcgg gcaatactgc gtgagaattt cctcaaagca
    10681 ggcatcgcct gcttcgttaa cgacagggta gaaaggtaga agttatgatt cgcacgatgc
    10741 tgcagggcaa actccaccgc gtgaaagtga ctcatgcgga cctgcactat gaaggttctt
    10801 gcgccattga ccaggatttt cttgacgcag ctggtattct cgaaaacgaa gccattgata
    10861 tctggaatgt gaccaacggc aagcgtttct ccacctatgc catcgcggca gaacgcggtt
    10921 cgagaattat ttccgttaac ggtgcggcgg cccactgcgc cagcgtcggc gatattgtca
    10981 tcatcgccag cttcgttact atgccagatg aagaagctcg cacctggcga cccaacgtcg
    11041 cctattttga aggcgacaat gaaatgaaac gtaccgcgaa agcgattccg gtacaggttg
    11101 cctgataatc acccctgcgg ctggttactc accagccgcg acatcgtctc gagcgaatcc
    11161 gttcttaaga tataaagtcg ttttagtaac aacggattat cccccggttt tactttgcct
    11221 ttcatggttg tcaccgccag gtgaaatccg gcttcttttg ctgccttcac ggctttgtca
    11281 ttaaatccgc caaacggata cgaaagatac cagacatgcg gattaaattg cgccagagcg
    11341 cggcgtgaac gtgcaaaatc aaacagaata ttgtgctcac tacggctcag caatatgggt
    11401 cggcgataac catccacccg atgcaaaaaa tgggtatgtg actggaaatc aaatacatcg
    11461 cgaatttcgt taagttcaga aacgctcata aattgcagcg attttgggtc ccacttctgc
    11521 gggtgacgtt tgatgcgcga ggtaacaata aacgccgtcg ccttcatgcc atattgtttc
    11581 aacacaggat acgcatagcg gctcaccgat ttgaggccat catcaaaggt aatcaccacc
    11641 gctcgcgcag ggagattgat cttattcttc acgtaacctt ccagctgcac catactcagt
    11701 gtcgtgtatc ccctgtcgcg cagccaggcc atctggttat tgaaagcgcg taccgaggtg
    11761 gtcgtcgaag tatggcgaaa acgggtgttt tcttcgtcgc gcagaatatg gtgataggtt
    11821 agcaccggca ggccattatc gggttgggca tccagcgcgc tgatataggc cagtcgatcg
    11881 ccaatacgga tctgatacca ggtttgattt aacctgtctt tcagtttatg caaaatcggg
    11941 tagcgcaaat tgtccgccag taccccaaac ggcgcactgc ccgcactcgg cgcgttatag
    12001 accggcgtat ctttccaggt aactaagttc tgattactca gcggcttgtt gaggtcaccc
    12061 aaaccgtctt caactttttg tcgcccctga accggctcga gatgaccttt atcgataaac
    12121 cctttaccaa agccaaaatt aaatgcgtaa taacttgcgg cagtgggctc cacggcaatg
    12181 atttgcccgg cccgaatatt ccccacggtc accatcttgt cgccaatttg cgcccagacc
    12241 gcagcatttt cgatggtttg catataacgg gcaggtaacg cggcactgac gcttgcggta
    12301 aacagcatca gcaggagaat aacagcttgt ttgtacatga aaaaataacc gagggtgaaa
    12361 caacgctttt attgtagcaa aagcgtcatc agaatcgcgg gttaatactc atacaaatta
    12421 tgtttgagga cgaaaggcgg atttttttgt tgctggtgcc aaagactact cacctccggc
    12481 gcacccagtt cgacaatacg ctcgcgaaac tctgcgctgg ataacgtttc acggtagacc
    12541 agcatctgct caattaacgg taacgtgacg ccagaaatca cttcgcaacg ggaatgttta
    12601 tggcttaata gcgaagccac gcggtacggt ggcgctccgg ctatatcggt taagaagatg
    12661 acaccttcac cagagtccgc ttcatgcaga gcatcgcaca tcatgcggct gagcatatta
    12721 gagcttaacc cacgccagaa attcacggcc cggcactgaa gaagtgcccc atgttttttc
    12781 tccagcgcat ccagtatctc ttgcgcccgg tcatcgtgac aggtaattac ccaacctaac
    12841 atcgccttcc tccttagccg aaaatcagtc taccctgacc attgttgata aagggtgata
    12901 gccgtcaaaa ctcctctccc ccaaatccag aggagaggaa atagccttag ctacgcaaac
    12961 cacgtccacg ttggatcagc gaccaacaga tcaaataaaa cgccacaata aagaccacca
    13021 gtacaccgaa ggtagtgacc agcggaacat cattgatacc gaggaagccg tagcggaaac
    13081 cactgatcat ataaacgatt gggttcaggt gcgacagccc ttgccagaac ggcggcaaca
    13141 aagtcagtga gtaaaagacc ccgcccaaat acgtaagtgg cgttaacaca aaggttggca
    13201 ccaggctgat gtcatcgaac gttttggcaa acacgccgtt cagcaaaccc gcaagggaga
    13261 acaacaccgc cgtgagcacc agcgttaaag cgacaaatac ccacgaatgc acctgaaacg
    13321 gcacaaaaaa cagtgaaatt gccgtcacca gaatgccaac aaacagacca cgcgccacgc
    13381 caccgccgac atatccggca ataatgacgt gagtcggaac cggcgctacc agcagctctt
    13441 caatattacg ctgaaacttg gcaccaaaaa atgatgacgc gacgttagcg taggcgttgg
    13501 tgatgaccga catcatgatc agccccggta caatgaactg catgtagctg aagccatgca
    13561 tatcgccaat acgcgaacca atcaggttac cgaagataat aaagtaaagg gtcatggtga
    13621 tgaccggtgg caccagcgtc tgcacccaga tacgcataaa gcgatggatc tctttcgccc
    13681 agatgctttt tagtgccacc cagtaaagat gcatcatgcg cgatctcctt gtttttcatt
    13741 aaccagtgaa acaaacagct cttccagacg gttagctttg ttacgcatac ttaatacctg
    13801 aatgccctgc tcacttaact gcgtaaatac gctgttgatc ccctgctcac gcagaacttc
    13861 aacttccagc gtcgcggtat cgaccagtcg atactgatag ccatcgagct tcggtaacgg
    13921 gcttttcggt gcgagatcga gaataaaggt ttccgatttc agcttcgcca gcagcgcctt
    13981 catcgaggta ttttccacca gctcaccgtg ttgaataatg ccgatattgc ggcacagcat
    14041 ttctgcttct tccaggtagt gtgtggtgag aatgatagtg gtgcctttgt cgtttaaatc
    14101 cttcaaaaag ccccacattg agcggcgaag ttcgatatcc acgcctgcgg tcggttcgtc
    14161 gagaatcagt agtttaggtt catgcattaa cgcacgggca atcattaaac ggcgcttcat
    14221 cccgccagat aacatacgcg cacgttcgtt gcgttttccc catagatcga gttgtttaag
    14281 atacttttcg ctgcgaatgt acgcttcttt gcgctccaca ccgtagtacc ctgcctgatt
    14341 caccacaatt tgctgcacgg tttcaaacgg gttgaagtta aattcctgtg gcaccagacc
    14401 caactgacgt ttagcgttta cgacatcttt ctcgagatcg taaccaaata cgctgacccg
    14461 cccggaggtt ttatttacca gagagctgat aataccgata gtggtcgatt ttccggcccc
    14521 gttcggcccg agaagcgcat aaaaatcacc cgcttcgacc tgcaaatcga ttccacgaag
    14581 cgcctgaacg ccgcctggat aggttttttt aagctgttga agttccagtg caatggtcat
    14641 aaatttttac ttaccttacg ttcttacact ttatctgtgg tttaaatcgt cccggagttg
    14701 ccctatatta gccaaacgta attatttggt tacaggtcgt taacctccat gaaagacata
    14761 gatacactca tcagcaacaa tgcactatgg tcaaaaatgc tggtggaaga ggatcccggg
    14821 ttttttgaga aactggcaca agcgcaaaaa ccgcgctttc tatggattgg atgttccgac
    14881 agtcgcgttc ctgcagaacg tttaaccggt cttgagccgg gcgaactctt tgttcaccgt
    14941 aatgttgcta acctggtcat tcacaccgac ctgaactgcc tttccgtggt tcagtatgca
    15001 gtggatgtac tcgaagttga acacattatt atctgtggcc actacggttg cggcggcgta
    15061 caagccgcag ttgaaaaccc ggaactgggg cttatcaaca actggctgct gcatatccgc
    15121 gatatctggt tcaaacatag ctcattgctc ggcgaaatgc cgcaagagcg ccgtctggat
    15181 accttgtgtg aactgaacgt catggaacag gtgtataacc tgggccactc caccattatg
    15241 caatcagcgt ggaaacgcgg gcagaaagtt accattcacg gctgggccta cggcattcac
    15301 gacggcttgc tgcgtgatct ggatgtcaca gcgactaacc gcgaaaccct tgagcaacgt
    15361 taccgtcacg ggatctccaa cctcaagctg aaacacgcca accacaaata aaaatgccat
    15421 gccggatgca acacatccgg caacttcaca cttactcgtc cagcagaatc actttgccga
    15481 tatacggcag atgacggtaa cgctgtgcgt aatcaatgcc gtaacccacc acaaactcat
    15541 ccgggatcga gaaaccgata aattctaccg ggacgttcac ttcacgacgg gacggtttat
    15601 ccagcagcgt acaaatcgcc aacgacttcg gttcgcgcag gcttaagatc tcacgcactt
    15661 tcgacagtgt attccccgag tcgatgatat cttcaacaat cagcacgtcc ttgccacgga
    15721 tatcttcatc cagatctttg aggattttca catcacgggt ggtggacatg ccgctaccgt
    15781 agctggaggc ggtcataaaa tcgacttcat gagatacctg aacttcacgg cacaggtccg
    15841 ccataaacat aaatgagcca cgcagcagac caaccagcac catatcgctg ccactgtctt
    15901 tgtaacgctc agtaatctga cgacccagtt cggcgatacg cgctttaatc tccgcttcgg
    15961 ggatcattac ttctacagta tgtttcatat ctctaaccat atgatttaaa aacaaatcac
    16021 ttcgcacggg caggtttcag caccgcaaca aagcgcaaga cacgcagtat accagtaaaa
    16081 ctattcattc gcggaatctg ttaataaagc tcgaattgtg atgacgatca cacatgttaa
    16141 aacctataat taattgctac taaaatatta atgaattgaa atggtgtctc tttatggcaa
    16201 ttaacaatac cggctcgcgg cgattactcg tcacgctaac agcccttttt gcagcgcttt
    16261 gcgggctgta tctactcatt ggcggaggct ggctggtcgc gattggcggc tcctggtact
    16321 accctatcgc tggccttgtg atgctcggcg tcgcctggat gctgtggcgc agtaaacgcg
    16381 ccgcgctttg gctgtacgca gccctgctgc tcggcactat gatttggggc gtctgggaag
    16441 ttggtttcga cttctgggcg ctgactccgc gcagcgacat tctggtcttc ttcggcatct
    16501 ggctgatcct gccgtttgtc tggcgtcgcc tggtcattcc tgccagcggc gcagttgccg
    16561 cactggtggt ggcactgctg attagcggtg gtatcctggc ctgggccgga tttaacgatc
    16621 cgcaggaaat cagcggcacc ttaagcgccg ataccacgcc agcagaagcc atctcccccg
    16681 ttgccgatca ggactggcct gcctatggtc gcaatcagga aggtcaacgc ttttcacctc
    16741 tgaaacaaat taacgctgat aacgtccaca agctgaaaga agcctgggtg ttccgtactg
    16801 gcgatgtgaa gcagccgaac gatccaggtg aaatcaccaa tgaagtgacg ccaattaaag
    16861 tgggcgacac cctttacctg tgtaccgctc accagcgcct gtttgcgctc gatgccgcca
    16921 gcggcaaaga gaaatggcat tacgatcctg agctgaaaac caacgagtcc ttccagcatg
    16981 taacctgccg tggcgtctct tatcatgaag ccaaagcaga aactgcttcg ccggaagtga
    17041 tggcggattg cccgcgtcgt atcattcttc cggtcaatga tggtcgtctg attgcaatta
    17101 acgctgaaaa cggcaagctg tgcgaaacct tcgccaataa aggcgtgctc aatctgcaaa
    17161 gcaatatgcc ggacaccaaa ccgggtctgt atgaaccgac ttcgccgccg attatcaccg
    17221 ataaaaccat cgtgatggcc ggttcagtca ccgataactt ctcaacccgt gaaacatctg
    17281 gcgtgatccg tggttttgat gtcaacaccg gggagctgct gtgggctttt gatcctggtg
    17341 cgaaagatcc taacgcaatc ccgtctgacg aacacacctt tacctttaac tcgccaaact
    17401 cgtgggcacc agcggcctat gacgcgaagc tggatctggt gtatctgccg atgggcgtga
    17461 ccacgccgga tatctggggc ggtaaccgca caccggaaca ggaacgttat gccagctcga
    17521 ttctggcgct gaatgccaca accgggaaac tggcgtggag ctaccagacc gttcaccacg
    17581 acctgtggga tatggatctt ccggcacagc cgacgctggc ggatatcacc gttaatggtc
    17641 agaaagtgcc agttatttat gctccggcga aaaccggcaa catttttgtg ctcgatcgtc
    17701 gtaatggcga actggtggtt ccggcaccgg aaaaaccggt tccccaaggt gctgccaaag
    17761 gcgattacgt aaccccaact caaccgtttt ctgaactgag cttccgtccg acgaaaaatt
    17821 tgagcggtgc ggatatgtgg ggagccacca tgtttgacca gctggtgtgc cgcgtgatgt
    17881 tccaccagat gcgctatgaa ggcattttca ccccgccatc tgaacagggt acgctggtct
    17941 tccctggtaa cctggggatg ttcgaatggg gcgggatttc cgttgatccg aatcgtgaag
    18001 tggcaattgc caacccaatg gcactgccgt ttgtttcgaa actgatccca cgcggtcctg
    18061 gcaacccgat ggagcagccg aaagatgcca aaggcacggg tacggaatcc ggcattcagc
    18121 cgcagtacgg tgtaccgtat ggcgtcacgc tcaatccgtt cctctcaccg tttggtctgc
    18181 cgtgtaaaca gccagcatgg ggttatatct cggcgctgga tctgaaaacc aatgaagtgg
    18241 tgtggaagaa acgtattggt acgccgcagg acagtatgcc attcccgatg ccggtacctg
    18301 tgccgttcaa tatgggtatg ccgatgctgg gcgggccaat ctccacggcg ggtaacgtgc
    18361 tgtttatcgc cgctacggca gataactacc tgcgcgctta taacatgagc aacggtgaaa
    18421 aactgtggca aggtcgttta ccagcgggtg gtcaggcgac gccaatgacc tatgaagtga
    18481 atggtaagca gtatgtggtg atctccgcag gcggtcacgg ttcatttggt acgaagatgg
    18541 gcgactatat tgtggcttat gcgttgccgg atgatgtgaa gtaaggcttg ctcagattgc
    18601 tgacaacgtg cgatttgttc atgccggatg cggcgtgaac gccttatccg gcctacaaaa
    18661 catttgcaaa ttcaatacat tgcgcgtagg cctgataagc gtagcgcatc aggcagtttt
    18721 gcgtttgtca tcagtttcat gcccggagag atccgggcat atttccgaat acggtctttt
    18781 tataccgtaa accctaacat catccccgta tcttcatgct ccagcaaatg gcagtgcgcc
    18841 atataagcat gttctttcgg tgcatcgtga ttaaacttca ccagcacttc gctgacatta
    18901 ccttctacct taacggtatc tttccagcct gcgcgatgcg tcgctggcgg tttgccattt
    18961 tctgacaaga tacggaactg cgtaccgtgg atatggaacg gatgcagcat catgtcgccc
    19021 acgccagaga taacccaacg ttcgtattgc cctttcgccg ccgcaaacat cggcttgttc
    19081 atatcaaacg cctgaccgtt gattttattg gcatggtgga aatcgaactt cccgccgtgg
    19141 ttcatatgat tcatattgcc gtgccccata tggcccatca tctggctgtg atccatcccg
    19201 gccatcgcct gatcgccata tttctccatc agcatctgca tccccatcat atcgagcatt
    19261 gggtccatag aaagttgcag cttgcgtacc gtcagccctt ccagcgaagg taacgcaggc
    19321 aggctactta atgtgtctgg caaagcacca gaggcactaa tggcaatcgg ctgaatccgc
    19381 attaccggat gcggcttgtc aaacggcgca atcgccatcc ccatctggct gaccggcagc
    19441 gtcaccaggt caaagggttt gttgtcgtta acctccacca gcacttcaaa acgctcgccc
    19501 atcagcactg gcagctcgtt caccttcacc ggttcaggta gcagaccgcc gtcgctggca
    19561 atcacataaa gcgggcgatt gtcgctggtg gcgaaattga gcgagcgggc attacagcca
    19621 ttgagcaaac gcaggcgcag ccagccacgc ggggcagcgt gttgtgggta gattacaccg
    19681 ttggtcagca acgtatcgcc aaaccaaccc acagcggcgg tcatcacatc cagttgataa
    19741 tcaatctgcc cgtcagcgtt aaatttttta tcctgaacaa taaccggaac atcatcaata
    19801 ccccactgtt ttggcagcat taatttcagg atctcgtcat cttcaatcac caccaaacca
    19861 gccagcccca tcgccacctg acgtccggtc ttgccatgtt gatgtggatg gaaccagcag
    19921 gtagcggcag gttgatcaac gttcaacgtc accgagcgct tgccacctgg cggaataatt
    19981 ccctgcgggc cgccgtccac ttcacccggt acttccagcc cgtgccagtg caacgtcgtc
    20041 tcttccgtca gttggttgta gatatcaacc gttaccgctt tgccgcgctg taatttcacc
    20101 gccggcccca gcagattgcc gttatagccc caggtagttg cggttttccc gccaaaggtg
    20161 gactgacctg cgccaatagt taactgaatg cgattacggg catcggtcgt gagcaaatca
    20221 gggatcggta acgttgggcg ttccgccgca aatactgcgc ggctccacag cggcaaagcc
    20281 gaagccacgc ccagcgcgac ggaatatttt aagaaatcac gacgttgcat agttatttcc
    20341 ttattcttaa gcaggcgaaa caaaatcaaa cgttgagcat agtccttccc cttacgggaa
    20401 ggtcaagccg cagacacaat ttaatcgaca ataatggtcg tcgcctcgct cgcagtgtgc
    20461 taacgtttat cttctttaag cccctggtag aagcaatgaa gacgtttttc agaacagtgt
    20521 tattcggcag cctgatggcc gtctgcgcaa acagttacgc gctcagcgag tctgaagccg
    20581 aagatatggc cgatttaacg gcagtttttg tttttctgaa gaacgattgt ggttaccaga
    20641 acttacctaa cgggcaaatt cgtcgcgcac tggtcttttt tgcccagcaa aaccagtggg
    20701 atctcagtaa ttacgacacc ttcgacatga aagccctcgg tgaagacagc taccgcgatc
    20761 tcagcggcat tggcattccc gtcgctaaaa agtgcaaagc cctggcccgc gattccttaa
    20821 gcctgcttgc ctacgtcaaa taatccctga tacctttttg tagaaatatt gaccgtgcat
    20881 ccgcggtcaa tgttagctat tatgttgcgc ccttttttta cgggtgttaa caaaggaggt
    20941 atcaacccat ggccgaaaaa aaacagtggc atgaaacgct acacgaccag tttgggcagt
    21001 actttgcggt agataacgtt ctgtatcatg aaaagaccga tcaccaggat ctgatcattt
    21061 ttgagaacgc cgcatttggt cgcgtaatgg cgctggatgg cgtagtacaa accaccgagc
    21121 gcgacgagtt tatctatcat gagatgatga cccatgttcc gctactggcc cacggtcacg
    21181 cgaaacatgt gctgattatc ggcggcggcg acggtgccat gctgcgtgaa gtaacccgac
    21241 ataaaaacgt tgagtcaatc acgatggtgg aaatcgatgc gggtgtcgtg tcgttctgcc
    21301 gtcagtatct acccaaccat aacgccggta gctacgacga tccgcgcttt aaactggtga
    21361 tcgacgatgg cgtcaatttc gttaatcaaa ccagccagac ctttgatgtc attatttccg
    21421 actgcaccga tcctatcggt cccggcgaaa gtctgttcac ctcagcattt tatgaaggct
    21481 gcaaacgttg cctgaatcct ggcggtatct tcgtcgcaca aaacggcgtc tgctttttac
    21541 agcaggaaga ggccatcgac agccatcgca aactcagcca ttacttcagc gatgttggct
    21601 tttatcaggc ggcgatcccg acctattacg gcggcatcat gacctttgca tgggcgacag
    21661 ataacgacgc cttacgccat ctctcaaccg aaattattca ggcgcgtttt ctcgcttctg
    21721 gcctgaaatg ccgttattac aatccggcag tccatacggc agcttttgcc ttaccccagt
    21781 atctgcaaga tgcactggct tcacagccgt cctaaggaga agataagaaa ttgaaaaaac
    21841 tgaaactgca tggctttaat aatctgacca aaagtctgag tttttgtatt tacgatatct
    21901 gctacgccaa aactaccgaa gagcgcgacg gttatattgc ttatatcgat gaactctata
    21961 atgccaaccg tctgaccgaa atcctgtcag aaacctgttc cattatcggg gccaatattc
    22021 ttaacatcgc ccgccaggat tacgagccac aaggtgccag cgtcactatt ctggtgagtg
    22081 aagaaccggt cgacccgaaa cttatcgaca aaacagaaca ccccggccca ctgccagaaa
    22141 cggtcgttgc ccatctcgat aaaagtcata tttgcgtaca tacctacccg gaaagtcatc
    22201 ctgaaggcgg tttatgtacc ttccgcgccg atattgaagt ctctacctgc ggcgtgattt
    22261 ctccgctgaa ggcgctgaat tacctgatcc accagcttga gtccgatatt gtgactattg
    22321 attatcgcgt gcgcggtttt acccgcgaca ttaacggtat gaagcacttt atcgaccatg
    22381 agattaattc gattcagaac tttatgtctg acgatatgaa ggcgctgtat gacatggtgg
    22441 atgtaaacgt ctatcaggaa aatatcttcc ataccaagat gttgcttaaa gagttcgacc
    22501 ttaagcacta catgttccac accaaaccgg aagatttaac cgacagcgag cgccaggaaa
    22561 ttaccactgc gctgtggaaa gaaatgcgcg agatttatta cgggcgcaat atgcccgctg
    22621 tttaacgtct caggcggagc acggaggctc cgcctgattt acttacttct gctgcacgaa
    22681 attacggtaa gccgccacca cctgaagaaa atcctcaacg ccgcacagcg acaagctctc
    22741 ttcatcgtag tagttcatcc cctcttccat ttcatcgcca gcgaattcca gttgattggc
    22801 gcgaaccatc acctcttcac catccatcca tagcgtgtat tcatgccctg cccgttgcca
    22861 ggaacgttcg ctacctttca atgcgtgcgc cgcttgttct acttcatcaa gcaaggccag
    22921 gttttctttc acctcttcat taaaccagtg cccgaccact tcatgcccca tggacatgcg
    22981 cacctttacc actccggtaa tatcgcgcag aaattcgtaa tccatagtgt gttcctctgc
    23041 tcctggcaat gcgccgaaat cgcattgcgc cattgatgta attatcgcag ccttaccgga
    23101 gaaaaacagc ggaacggcga tggtttagaa ataaaaaatg cccgggaatc tcattcaccg
    23161 ggcattgtgt cgtttatgcg cagcgcgtgc gcttacttat taaaccgcag tctggaaaat
    23221 caccccgtcc gctttttcgg tgtactgaga aagctggtcg aagttcagat aacggtaagt
    23281 atcaacggct gttttatcta cctgcgccac gtaggtctgg tactcttccg gcgtcggcag
    23341 tttgccaatc agcgccgcaa cagcagccag ttccgcagaa gccaggaaga cattcgcgcc
    23401 agtacccaaa cggttcggga agttacgggt agaggtggaa accaccgtcg caccgtctgc
    23461 tacacgcgcc tggttaccca tacacaggga acagccaggg atctcgatac gagcaccact
    23521 cttaccgaag acgctgtagt agccttcttc ggtcagctgc gcagcgtcca tacgcgttgg
    23581 cggtgccacc cacaggcggg tcggcaactg acctttgtgc gcatccagca gtttaccagc
    23641 agcacggaag tgaccgatgt tagtcatgca agaaccgata aatacttcgt cgatcttctc
    23701 gccctgtacc gcagacagcg gacgtgcgtc gtccgggtca ttcggtgcac agaggattgg
    23761 ctctttgata tccgccagat cgatgtcgat cactgccgcg tattccgcat ctgcatcggc
    23821 ttccagcagc tcaggattcg ccagccattt ttccatgccc tgaatacgac gctccagggt
    23881 acgacgatcg ccgtaacctt ccgcgatcat ccacttcagc aggacgatgt tagagttcag
    23941 gtattcaatg atcggttctt tgttcagctt gatggtacaa ccggcagcag aacgctcggc
    24001 agacgcatcg gtcagctcaa atgcctgctc aactttcaga tccggcagac cttcaatttc
    24061 cagaatgcgg ccagagaaga tgtttttctt acctttcttc tcaacggtca gcagaccttg
    24121 tttgattgca tacagcggga tcgcgtgtac cagatcgcgc agggtgatac ccggctgcat
    24181 tttgcctttg aagcgcacca gaacggattc tggcatatcc agcggcatta cgcctgttgc
    24241 ggctgcaaat gccaccagac cagaacccgc cgggaaagag ataccgatcg ggaaacgggt
    24301 atgggagtca ccaccggtac cgacagtatc cggcagcagc atacggttca gccaggagtg
    24361 gattacgccg tcacccggac gcagcgacac accgccacgg ttcataatga agtccggcag
    24421 cgtgtggtgc gtgttaacgt caactggttt cggatacgcc gcggtgtggc agaaagactg
    24481 catcaccagg tcagccgaga agcccaggca cgccaggtct ttcagttcat cacgggtcat
    24541 cgggccggtg gtgtcctggg aacctacaga agtcattttc ggttcacagt acgcgcccgg
    24601 acgaatgcct ttcacgccac aggcacggcc taccattttc tgcgccagcg agaagccgcg
    24661 atcgctctca gcgacatctt tcgcctgacg gaacacatcg ctgtgcggca ggccaagtgc
    24721 ttcacgcgct ttggtggtca ggccacgccc aataatcagc ggaatacggc cgccagcacg
    24781 cacttcatca atcagcacgt cggttttcag ttcgaaggtc gccagcagtt cgccggtttc
    24841 gtggttacgc acttcacctt tgtacgggta aacgtcaatt acgtcgccca tgttcaggtt
    24901 agagacgtcg acttcgattg gcagcgcacc cgcgtcttcc atcgtgttaa agaagatggg
    24961 tgcaatttta ccgccgaggc acaaaccacc gccgcgtttg ttcggcacat gtggaatatc
    25021 atcgcccata aaccacagaa cggagttagt ggcggattta cgcgatgacc ccgtacccac
    25081 aacgtcaccg acgtacgcca gtgggaaacc tttctgttgc agagcttcga tttgcttaat
    25141 cggaccaaca acgcctggct gatctggctc aataccttca cgggcgtttt tcagcatcgc
    25201 cagcgcgtgc agtgggatat ccgggcgtga ccacgcatcc ggtgccggag agaggtcatc
    25261 ggtgttggtt tcgcctgtga ctttgaagac ggtaacggtc agtttttcag ccagcgccgg
    25321 gcgattcagg aaccattcgg catccgccca ggactgcatt acctgcttcg catattcgtt
    25381 gcctgctttc gctttctctt ctacgtcata gaagttatcg aacatcagca gcgtgtgaga
    25441 aagtgctttg gcagcgatcg gtgccagttt ggcatcatcc agcgcgtcga tcagcggatg
    25501 aatgttgtaa ccaccctgca tggtgcccag cagttcgatg gctttttccg gagtcagcag
    25561 aggggatttg gcttcgcctt tagcgatagc agccaggaag cctgctttta cataggcggc
    25621 ttcatcgacg cctgggggaa cacggttggt taacagatct aacaggaatt cttcttcgcc
    25681 cgcgggcggg tttttcagca gctctacaag cgcggccatt tggtttgcat ccaggggttt
    25741 gggcgcaatc ccctcagcgg cacgctcagc tacgtgctta cggtattctt ctagcacgac
    25801 ggttctcctc gctctcattg tcatagtgcg gcaggcgatt ctcttcacgc tcctgtgaga
    25861 cagcagtttg tagggtaaat gcccagtacc gcaacgggca gaatagcagg attttgacga
    25921 tgtgttaatc tgtttacaaa aaagcaacat aaaaaagtgg ctgaatcgtt aaggatggtc
    25981 tagagattgt ttccctccgt aaaaacttcg ttaattcgca tggtgataat cacctttctc
    26041 aacgaatccc aagattagtc aaaatttaaa ctaccgcctc tttatactcg gattcacagc
    26101 acctgcgggt ggcagttcgc cgaccattgc gatttccttg agatccgaat tatgaaaatg
    26161 actttgccgt ttaaacccca tgtgctggcg ctaatttgca gtgccgggct ttgtgccgcc
    26221 tctaccgggc tatatataaa aagccgcaca gtggaagcgc ctgtggaacc gcaatcgaca
    26281 caactggctg cgcctgacat ctccgcagtt acgcttcctg caacggtttc cgtgcctcct
    26341 gtaacacctg ccgtcgtcaa atccgcattc agcactgcgc aaatagatca atgggttgca
    26401 cctgtcgcgc tataccccga ctctctgctt tcacaagtgt taatggcatc aacctatccg
    26461 gcaaacgttg ctcaagcagt gcaatggtcg cacgataatc cacttaaaca aggcgatgct
    26521 gctattcagg cggtatctga ccagccctgg gacgccagcg tcaaatcact ggtggccttt
    26581 ccacagttga tggcattgat gggcgaaaac ccgcaatggg tgcaaaacct gggcgatgct
    26641 tttctggcgc agccgcagga ggtgatggac tcggtacagc gattgcggca actggcgcaa
    26701 caaaccggct cgctgaagtc atcaactgaa cagaaagtta ttaccacaac gaagaaagct
    26761 gtaccggtaa aacagacagt cacggctccc gtcataccat ccaataccgt ttcaactgcc
    26821 aaccctgtca ttacagagcc tgcaacaacc gtcatttcca ttgaacccac caatcctgat
    26881 gtggtctata ttcccaacta caacccaacc gtggtttacg ggaactgggc caatactgcg
    26941 tatccgccgg tttatctgcc gccgccagcc ggagaaccgt ttgttgacag ctttgtgcgc
    27001 ggattcggct atagcatggg cgttgctacc acgtacgcgc tattcagcag catcgactgg
    27061 gatgacgacg atcatgacca tcatcatcat gacgatgatg attatcacca tcacgatggc
    27121 ggtcatcgtg atggtaatgg ctggcaacac aatggcgaca acatcaatat cgacgtcaac
    27181 aatttcaacc gtatcaccgg tgaacatctt actgataaga atatggcatg gcggcacaat
    27241 ccaaactacc gtaatggtgt gccctatcat gatcaggata tggcaaagcg gtttcatcaa
    27301 accgatgtca acggcggaat gagcgccacg cagctacctg ctccaacacg cgacagccag
    27361 cgtcaggcgg cagcaagtca gtttcagcaa cgaacacaca gcgcaccagt cattacacga
    27421 gatacccaac gtcaggcagc ggcacagcgg tttaatgaag ctgaaaacta tgggagctat
    27481 gacgacttcc gcgacttcag ccgtcgccaa ccactgaccc agcaacaaaa ggacgccgct
    27541 cgtcagcgtt atcagtcggc ctctcctgag cagcgccagg cagtccgcga gaaaatgcag
    27601 actaacccgc agatccagca gcgaagagag gcagcgcgtg agcgcattca gtctgcaact
    27661 cccgaacagc gccaggtgtt taaggaaaaa gtacagcagc gcccactgaa tcaacagcaa
    27721 cgtgataacg cccgccagcg tgttcaatca gcatcacctg agcaacgtca ggtttttcgg
    27781 gagaaagttc aggagagccg cccgcaacgt ctaaacgaca gtgaccatac tgtcaggctg
    27841 aataacgagc aacggtcagc agtacgcgaa cgtctctctg agcgcggagc aaggcgactg
    27901 gaaaggtaaa ttacagacgt aaaaaaagcg gcgtggttag ccgctttttt aattgccaga
    27961 tgttccggca aacgaaaaat tacttcttct tcgctttcgg gttcggcagg tcggtaatgc
    28021 taccttcgaa cacttctgcc gccaggccca cagactcgtg cagagtcggg tgcgcatgga
    28081 tggtcagtgc gatgtcttca gcgtcacaac ccatttcgat tgccaggccg atttcaccca
    28141 gcaactcgcc gccgttagtg ccgacaatcg caccaccgat cacacggtga gattctttgt
    28201 cgaaaatcag cttggtcata ccgtctgcgc agtcggaagc gatagcacga ccagaagcag
    28261 cccacgggaa ggtggcggtt tcgtagctga tgcctttctc tttcgcttct ttctcagtca
    28321 gacctaccca tgcaacttct ggttcggtat aggcgatgga cgggataact ttcggatcga
    28381 agtaatgttt tttaccggcg ataacttcag cggcaacgtg accttcgtga acacctttgt
    28441 gtgccagcat tggctgaccg acgatatcac cgatagcaaa gatgtgcggt acgttggtac
    28501 gcagctgttt gtcaacgcgg atgaaaccac ggtcgtcaac ttccacgcca gctttgcctg
    28561 cgtcgaggtt tttaccgttc ggcacacgac caatcgctac cagcacggcg tcgtaacgct
    28621 gcggttcagc gggtgctttt ttgccttcca tcgtcacata aatgccgtct tctttcgctt
    28681 caacggcggt aactttggtt tccagcatca ggttgaattt cttgctgata cgcttggtaa
    28741 agactttaac gatgtcttta tcagctgccg ggatgacctg gtcgaacatt tcaaccacgt
    28801 caatctgtga acccagcgcg tggtatacgg tgcccatttc cagaccgatg ataccgccac
    28861 ccattaccag caggcgttct ggtacttctt tcagttccag cgcgtcagtg gagtcccaga
    28921 tacgcggatc ttcatgcgga ataaacggca gttgaatcgg gcgagaaccc gctgcaatga
    28981 tcgcgttgtc gaagttgatc acggttttac cgttctcacc ttcaacttcc agggtgttag
    29041 ccccggtaaa tttacccaga ccgttgacca ctttgacttt gcggcctttc gccataccag
    29101 ccagaccacc ggtcagctga ttgatcactt tctctttcca ggtacgaatc ttgtcgatat
    29161 cggttttcgg ttcgccaaag acgataccgt gttcagccag cgctttggct tcttcgataa
    29221 cttttgctac gtgcagcagt gctttagaag ggatacagcc gacgttcagg caaacaccgc
    29281 caagggtgtt gtagcgttct acgattacgg tttccagacc taaatcagcg caacggaagg
    29341 cagcggagta acctgcgggg cctgccccaa gtaccacgac ctgagttttg atttcagtac
    29401 tcatcatgac ctctatatat ttatctccgg cggtcatacc cgtcgtcttt caggccgcag
    29461 cggcgttagc ttcacgccct caccccagtc acttacttat gtaagctcct ggggattccg
    29521 gcgcttgcta ccttgctgca cccagaaatc catagggtat gtgaagtaat accctaacca
    29581 ccaccgggtc gttctatccg tcggtatttt acaaaattgt taacaatttt taaacaacaa
    29641 acggcaaccg atttgtctat tcgctaataa cctcaataca ttcatgagat taccagaaaa
    29701 aagccggccg ttgggccggc tcttttactt acatcaccag acggcgaatg tcagacagcg
    29761 tgttgttaat gatggtaatg aaacgggcac catcagcacc gtcgatcacg cggtggtcga
    29821 aggagagaga aatcggcagc atcagacgcg gcacgaactc tttaccattc cacaccggct
    29881 ccatcgcgga cttggaaacg ccgaggatag ccacttccgg cgcgttcaca atcggcgcga
    29941 agtgggtagt acccaggccg ccgatgctgg agatggtgaa gcaaccgccc tgcatttcgc
    30001 ccgcagtcag cttaccgtca cgcgctttct tagaaatagt catcagctcg cgagacagct
    30061 cgatgatgcc tttcttgttg acgtctttga ataccggaac aaccagaccg ttcggggtat
    30121 ccaccgccac accgatgttg atgtatttct tcagggtcag acgctgaccg tcttccgaca
    30181 gcgaactgtt gaagcgaggc atctgctcaa gagctgcagc aacggctttc atgatgaaga
    30241 caaccggggt gatcttcaca tccagcttac gtttcgccgc ttcttcgttc tgctgtttac
    30301 ggaacgcttc cagctcggtg atatcggttt tgtcgaagtg ggtaacatgc gggatcatca
    30361 cccagttacg gctcaggttc gcaccagaga ttttctggat acggcccagt tccacttctt
    30421 cgatttcacc aaacttgctg aagtccacct tcggccacgg cagcatgccc gggataccgc
    30481 cgccagtcgc cgccggagcc gcttctgcac gtttgatagc ttctttcacg taagcctgaa
    30541 cgtcttcgcg caggatacga cctttacggc cagtgccctt cactttcgcc aggttaacgc
    30601 caaattcgcg tgccagacgg cggatcagcg gagtcgcgtg aacgtaagcg tcgttttcag
    30661 caaattcaga tttgccttcg gctttcgcag ccggagctgc tgctggagct tcagcttttg
    30721 ctgccggagc cggcgctgcc gcttcctgtt tcgcaggagc tgccgcaggc gctgcgcctt
    30781 caacttcgaa gatcataatc agcgagccag ttttcacttt atcgccaacg ttgactttca
    30841 gttccttcac gacgcctgca aacggagccg gaacttccat agacgcttta tcgccttcta
    30901 cggtgatcag tgactgttca gcggcaactt tgtcgcccac tttcaccatc acttcggtca
    30961 cttcaacttc gtcaccgccg atatccggaa cgttaacgtc tttcacgcca gccgctggtg
    31021 cagaggccgc tgccggagcc gcttcctgtt tagccgccgg agctgccgcg cctgcttcac
    31081 ccgcgacttc gaagaccata atcagcgagc cggtagacac tttgtcaccc acgttcactt
    31141 tgatctcttt cacggtgcca gcaaacggag ccggaacttc catagaagct ttatcgcctt
    31201 ctacggtgat cagcgactgt tcagcttcaa ctttatcgcc aactttcacc aggatttcgg
    31261 tcacttcaac ttcgtcgctg ccgatatccg gaacgttaac gtcttttgcc gctgcagccg
    31321 ctggagctgc tgccggagcg gcttctttct tctcttctgc ctgagcaggt gcagcgtctg
    31381 ctgcaccgtc ggcggaatcg aaaatcataa tcagtgcgcc ggtctgggtt ttatcgccaa
    31441 cagagacttt gatctcttta acgatacccg cctgcggaga cggaacttcc atagaggctt
    31501 tgtcgccttc tacggtgatc agcgactgtt cggcttcaac tttgtcgccc actttgacca
    31561 ggatctcggt gatttcaact tcatcagccc cgatgtccgg tactttgatt tcgatagcca
    31621 ttattctttt acctcttacg ccagacgcgg gttaacttta tctgcatcga tgttgaattt
    31681 ggcgattgcg tcagcaacca ctttcttatc gatttcgcca cgtttagcca gttcgcccag
    31741 cgccgcaacc acgacataag aagcatcaac ctcgaagtgg tgacgcaggt tctcacggct
    31801 gtcggaacga ccgaagccat cagtacccag tacgcggtag tcgtcagccg gtacgtaagt
    31861 acggacttgc tcagcgaaca gtttcatata gtcggtagat gccactgccg gagcgtcgtt
    31921 catcacctga gcgatatacg gaacgcgcgg agtttccagc gggtgcagca tgttccagcg
    31981 ttcacaatcc tgaccatcac gcgccagttc ggtgaaggaa gtcacgctat aaacgtcaga
    32041 acctacgccg taatctttcg ccaggatctc tgctgcttca cggacgtgac gcaggataga
    32101 accggagccc agcagctgaa ctttaccttt gctaccttca atggtttcga gtttgtagat
    32161 acctttacgg ataccttcct cagcaccttc cggcattgct ggcatgtggt agttttcgtt
    32221 cagcgtagtg atgtagtagt aaacgttctc ttgtttctca ccgtacatac gctccagacc
    32281 gtcatgcata atgacagcaa cttcgtaagc gtaagccggg tcgtaagaga tacagttcgg
    32341 gatagtcagc gactgaatgt ggctgtgacc atcttcgtgc tgcagacctt cgccgttcag
    32401 ggtggtacga ccggaagtac cgccgatcag gaagccacgc gcttgctggt caccagccgc
    32461 ccagcacaga tcgccgatac gctggaagcc gaacatcgag taatagatgt agaacggaat
    32521 catcggcaga ttgttggtgc tgtaagaggt cgccgctgcc agccaggaac acccagcgcc
    32581 cagctcgttg atcccttcct gcagaatctg acctttctcg tcttctttat agtaagcaac
    32641 ctgctcgcgg tcctgcgggg tgtactgctg accgttcggg ctgtaaatac caatctgacg
    32701 gaacagacct tccataccga aagtacgcgc ttcgtcggcg atgatcggta ccagacgatc
    32761 tttgatcgac ttgttcttca gcattacgtt cagggcacga acgaaagcga tagtggtaga
    32821 gatctctttg ctctgctctt ccagcagcgc gccgaagtct tgcaggctcg gcagctcaag
    32881 cttctcggtg aagttcggct gacggcttgg cagataaccg tgcagtttct gacgctgagc
    32941 gtgcagatag gtatgctctt cagaaccttc cgggaaggtg atgtacggca atttttcgat
    33001 atcggcatca gacaccggca cattgaaacg gtcgcggatg tgacgcacgc cgtccatgtt
    33061 cattttctta acctggtgcg cgatgttttt accttcagcc gcgtcgccca tgccgtaacc
    33121 tttaatggta tgagcaagga ttaccgtcgc tttgcctttg gtttcctgcg ctttcttaaa
    33181 tgcagcgtag attttcttcg gatcgtgacc accacggttc agtgcccaga tctgttcgtc
    33241 agtccagtct gcaaccagtg ctgcggtttc aggatattta ccgaagaagt gttcacgaac
    33301 gtacgcacca tctttcgatt tgaaggtctg gtagtcgccg tcaacggttt cgttcatcag
    33361 ctggatcagt ttaccgctgg tatctttacg cagcagttca tcccaacggc taccccacat
    33421 cactttgatc acgttccagc cagcaccttc gaagatgcct tccagttcgt tgatgatctt
    33481 gccgttaccg gtgaccgggc cgtcaagacg ctgcaggtta cagttgataa cgaaaaccag
    33541 gttatccagt ttttcacggg tagcgatggt gatcgcacct ttggattccg gttcgtccat
    33601 ttcaccgtcg ccgaggaacg cgtaaacggt ttgtttagag gtatctttca ggccacggtg
    33661 ttccagatat ttcaggaatt tagcctggta aatagcacca atcggcccca gacccataga
    33721 tacggtcggg aactgccaga attccggcat cagtttcggg tgcggatagg aagagaggcc
    33781 attgccgtga acttcctgac ggaagttatc cagctgctcc tgagtcagac gaccttccag
    33841 gaaagcacga gcgtaaacgc ccggggagat gtggccctgg aagtaaacca ggtcgccgcc
    33901 atcctgctcg ttgcgtgcac ggaagaagtg gttaaagcac acatcataaa tggttgcgga
    33961 agactggaag gacgccatgt ggccgcccag ttcgaggtct tttttcgacg cacgcagaac
    34021 cgtcatgatg gcgttccagc ggatagctga acgaatacgg cgttccagtt ccagattacc
    34081 cggatactcc ggttgttctt caacggggat ggtgttgatg tagttgctga taccggtgcc
    34141 tgcggctacg ttgacaccgc ctttgcgggc ttcagcaagc agttggtcga tcagatactg
    34201 agcacgctca acaccttctt cacggatgac cgattcgatc gcctggagcc agtcgcgagt
    34261 ttcgatcgga tccacgtcat ttgggaaacg ttctgacatg ggttattcct tatctatcta
    34321 ataacgttga gttttctgga acctgtccca ttgaactctc gccggaaagc tcaataagac
    34381 aggttctacg tttagttgcc gcgcttttat atgcgcttga tttacaacat cttctggata
    34441 atttttacca gaaaaatcac taattctttc gttgctccag acgacgtaga gaacgctcac
    34501 ggcggctctc ttcacggctt ctgtcgagca aaatttcttc gataaaggcc agatggcgat
    34561 gcgatgcttc gcgcgcttct tccggcttac cggccataat cgcttcaaat atgcgggtgc
    34621 ggtgactact caccagcggc agcatctcgc gacgcgaata gagcaattcg aagttctggc
    34681 ggacattctg ggccaacatc ggctccatac accttagcag atgaagcaga accacattgt
    34741 gggccgcttc ggtgacggca atctgatact ggagtacggc atttgattcc gcgtccagat
    34801 cgccagactg ctgcgccagc tctatggcat ggtggagttc acggatgcgt tccttgtctt
    34861 catcggtact acgcagcgcg gcgtaataag cggcgatacc ttccagggcg tgtcgtgttt
    34921 cgagcaagtc atactgtgat tcaggatggt cggagagcag ctccaccagc ggatcgctga
    34981 agctttgcca taggctgctc tggacaaaag tgccgccacc ctggcgacga agcaacaagc
    35041 ccttcgcttc gagacgttga atcgcctcac gcaaggaggg acgggagacg tcgaactgtt
    35101 ttgccagttc gcgttccggt gggagttttt cgcccgggcg gagagtgcct tcgaggatca
    35161 aaaactccag ttgctgctca atcacatcgg agagttttgg ttggcggatt ttgctgtagg
    35221 ccatgagttc ctgtcttaag ccacttgccg aagtcaattg gtcttaccaa tttcatgtct
    35281 gtgacgctaa agtaacaaag tattcacctt atgtccatac aggttttgat tgaaatcatg
    35341 aaactgtgca cattttaaca acttgacata tataacgttt caaagttgta actatgcaca
    35401 aatgttaaat ttacccatca aggggtgaga ttaacagaat tgaaacgaaa aaatatgtcc
    35461 tctgaaccga ttcaccaagc aacaaaacgc ataacaattc aattatgtga attaatatgt
    35521 aaaatgaatg ttcagggaag gagagaaagg tagtatttac ctatgttttc tttttacatt
    35581 gcttattatc actgcaatcg ccaccgtaac cttctcgtag cgaaaatcat cacttaacca
    35641 gaacgtgatg attttcaggt gacagattct gtttagaatt cattctgaat ctttgaacaa
    35701 agacaatcct tgcgaacgcc gccattcttt gacgattttc aggatgtttt caggcttatc
    35761 atctcccttc tttgcaggat agtagataag atcgctcatc aacggatgtt gtgtaattct
    35821 ggtaagatgt tcttctagat catcccaata tttgtcaagc tcttcccctt ttaattgagc
    35881 gttatccccg gttggatttg taaactcgcc aagcaactca atgaattccc gctctgtgta
    35941 atcttctact ttatctt
//
LOCUS       DAZGQL010000052        35747 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.052, whole genome shotgun sequence.
ACCESSION   DAZGQL010000052 DAZGQL010000000
VERSION     DAZGQL010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35747
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.052"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            73..1053
                     /gene="ydcK"
                     /locus_tag="ACHKIR_002768"
     CDS             73..1053
                     /gene="ydcK"
                     /locus_tag="ACHKIR_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcK family protein"
                     /protein_id="HFO9990482.1"
                     /translation="MRKYRLSEEQRAFSYQDDGTKKSVLLRQIIAMSDFNDVIAGTAG
                     GWIDCETVLAQEGNCWIYDQNAIAFGGAVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIRDSLVCGQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIEKHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            complement(1045..1584)
                     /gene="rimL"
                     /locus_tag="ACHKIR_002769"
     CDS             complement(1045..1584)
                     /gene="rimL"
                     /locus_tag="ACHKIR_002769"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HFO9990483.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNRTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNAQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDSQ"
     gene            complement(1647..1871)
                     /gene="ydcH"
                     /locus_tag="ACHKIR_002770"
     CDS             complement(1647..1871)
                     /gene="ydcH"
                     /locus_tag="ACHKIR_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HFO9990484.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQVSVKEV"
     gene            complement(2011..3666)
                     /gene="opgD"
                     /locus_tag="ACHKIR_002771"
                     /gene_synonym="mdoD"
     CDS             complement(2011..3666)
                     /gene="opgD"
                     /locus_tag="ACHKIR_002771"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HFO9990485.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTDFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            complement(3891..5234)
                     /gene="ydcJ"
                     /locus_tag="ACHKIR_002772"
     CDS             complement(3891..5234)
                     /gene="ydcJ"
                     /locus_tag="ACHKIR_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFO9990486.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQRGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGETHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQARSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            5451..6374
                     /gene="ydcI"
                     /locus_tag="ACHKIR_002773"
     CDS             5451..6374
                     /gene="ydcI"
                     /locus_tag="ACHKIR_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO9990487.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            complement(6412..8052)
                     /gene="trg"
                     /locus_tag="ACHKIR_002774"
     CDS             complement(6412..8052)
                     /gene="trg"
                     /locus_tag="ACHKIR_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HFO9990488.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRSVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILSKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVDVFRLNKHSVSAEPRGAGEPVSFATV"
     gene            8451..8600
                     /gene="hokB"
                     /locus_tag="ACHKIR_002775"
     CDS             8451..8600
                     /gene="hokB"
                     /locus_tag="ACHKIR_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HFO9990489.1"
                     /translation="MKRNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            complement(8578..8646)
                     /gene="yncP"
                     /locus_tag="ACHKIR_002776"
     CDS             complement(8578..8646)
                     /gene="yncP"
                     /locus_tag="ACHKIR_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YncP"
                     /protein_id="HFO9990490.1"
                     /translation="MNLLVRCAGKIPALALTWTCRP"
     gene            complement(8672..8845)
                     /locus_tag="ACHKIR_002777"
     CDS             complement(8672..8845)
                     /locus_tag="ACHKIR_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9990491.1"
                     /translation="MKKLALILVIGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCIN"
     gene            complement(9090..9620)
                     /gene="cybB"
                     /locus_tag="ACHKIR_002778"
     CDS             complement(9090..9620)
                     /gene="cybB"
                     /locus_tag="ACHKIR_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HFO9990492.1"
                     /translation="MGNKYSRLQISIHWLVFLLVIVAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHEMLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            9809..10810
                     /gene="gap"
                     /locus_tag="ACHKIR_002779"
     CDS             9809..10810
                     /gene="gap"
                     /locus_tag="ACHKIR_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFO9990493.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILAKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            complement(10851..12290)
                     /gene="aldA"
                     /locus_tag="ACHKIR_002780"
     CDS             complement(10851..12290)
                     /gene="aldA"
                     /locus_tag="ACHKIR_002780"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HFO9990494.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLRKISAGIRERASEISTLIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNRLGEAMQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEEAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(12487..13287)
                     /gene="ydcF"
                     /locus_tag="ACHKIR_002781"
     CDS             complement(12487..13287)
                     /gene="ydcF"
                     /locus_tag="ACHKIR_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF218 domain-containing protein YdcF"
                     /protein_id="HFO9990495.1"
                     /translation="MNITPFPTLSPATIDAINVIGQWLAQDDFSGEMPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIL
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFVNPLQGLWPVERYLSLLTGELPR
                     LRDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKHDAVLIEAMESRSLR"
     gene            complement(13559..17461)
                     /gene="hrpA"
                     /locus_tag="ACHKIR_002782"
     CDS             complement(13559..17461)
                     /gene="hrpA"
                     /locus_tag="ACHKIR_002782"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HFO9990496.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDHAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DPALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELSLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            17662..18267
                     /gene="azoR"
                     /locus_tag="ACHKIR_002783"
     CDS             17662..18267
                     /gene="azoR"
                     /locus_tag="ACHKIR_002783"
                     /EC_number="1.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HFO9990497.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAE"
     gene            complement(18321..19613)
                     /gene="ynbD"
                     /locus_tag="ACHKIR_002784"
                     /pseudo
     CDS             complement(18321..19613)
                     /gene="ynbD"
                     /locus_tag="ACHKIR_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415929.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
     gene            complement(19627..21384)
                     /gene="ynbC"
                     /locus_tag="ACHKIR_002785"
     CDS             complement(19627..21384)
                     /gene="ynbC"
                     /locus_tag="ACHKIR_002785"
                     /EC_number="2.1.1.-"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HFO9990498.1"
                     /translation="MENSRIPGEHFFTTSDNTALFYRHWPTLQPGAKKVIVLFHRGHE
                     HSGRLQHLVDELAMPDTAFYAWDARGHGQTSGPRGYSPSLASSVQDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVMVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIISDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRALVFEKMQ
                     SFISHLYANKSQKLDYQHEDCTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGA
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLVDKIYLNSVGWRGIRQRKTHLQ
                     MLIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDIFLRDYSELNVAQGQE
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIDPGGILIYTGQPWHPQLELIAGVLTSHKDGKPWVMRVRSQGEMDSLVHDAGFDKCT
                     QRIDEWGIFTVSMAVRRGN"
     gene            complement(21400..22296)
                     /gene="ynbB"
                     /locus_tag="ACHKIR_002786"
     CDS             complement(21400..22296)
                     /gene="ynbB"
                     /locus_tag="ACHKIR_002786"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFO9990499.1"
                     /translation="MLEKSLATLFALLILATLINRFLVWRLPERKGDEVTLRIRTWWG
                     IVICFSLVISGPRWVTLTFFALISFLALKEYCTLISVHFPRWLYWVIPLNYLLIGLNC
                     FELFLLFIPLFGFLILATWRVFVGEPSGFLHTVSAIFCGWIMTVFALSHAAWLLMLPT
                     TNIQGEALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLVGGVITTMI
                     VSLIIGPLLTPLDTLQALLAGLLIGISGFCGDVVMSAIKRDVGVKDSGKLLPGHGGLL
                     DRIDSLIFTAPVFFYFIRYCCY"
     gene            complement(22296..22901)
                     /gene="ynbA"
                     /locus_tag="ACHKIR_002787"
     CDS             complement(22296..22901)
                     /gene="ynbA"
                     /locus_tag="ACHKIR_002787"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFO9990500.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLHKRQVSANQITLAALALSLLTGL
                     LLALVPYHGMFLLLPIVLFVRMALNALDGMMARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGIRSYAGPFGKSDRALIFGLWVL
                     AVAIYPQWMQWNNLLWSIASILLLWTAINRCRSVLLMNAEK"
     gene            complement(23070..24410)
                     /locus_tag="ACHKIR_002788"
                     /pseudo
     CDS             complement(23070..24410)
                     /locus_tag="ACHKIR_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310036.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing bactofilin"
     gene            complement(24474..25334)
                     /gene="pdxI"
                     /locus_tag="ACHKIR_002789"
     CDS             complement(24474..25334)
                     /gene="pdxI"
                     /locus_tag="ACHKIR_002789"
                     /EC_number="1.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 4-dehydrogenase"
                     /protein_id="HFO9990501.1"
                     /translation="MSSNTFTLGTKSVNRLGYGAMQLAGPGVFGPPRDRHVAITVLRE
                     ALALGVNHIDTSDFYGPHVTNQIIREALYPYSDDLTIVTKIGARRGEDASWLPAFSPA
                     ELQKAVHDNLRNLGLGALDVVNLRVMMGDGHGPAEGSIEASLTVLAEMQQQGLVKHIG
                     LSNVTPTQVAEARKITEIVCVQNEYNIAHRADDAMIDALARDGIAYVPFFPLGGFTPL
                     QSSTLSDVAASLGATPMQVALAWLLQRSPNILLIPGTSSVAHLRENMAAEKLHLSEEV
                     LSTLDGISRE"
     gene            complement(25565..26155)
                     /gene="paaY"
                     /locus_tag="ACHKIR_002790"
     CDS             complement(25565..26155)
                     /gene="paaY"
                     /locus_tag="ACHKIR_002790"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation protein PaaY"
                     /protein_id="HFO9990502.1"
                     /translation="MPIYQIDGLTQFVPEESFVHPTAVLIGDVILGKGVYVGPNTSLR
                     GDFGRIVVKDGANIQDNCVMHGFPDQDTVVEEDGHIGHSAILHGCIIHRNALVGMNAV
                     VMDGAVIGENSIVGASAFVKAKAEMPANYLIVGSPAKAIRELSEQELAWKKQGTHEYQ
                     VLVTRSKQTLHQVEPLREVEPGRQRLVFDENLRPKQ"
     gene            complement(26137..26289)
                     /locus_tag="ACHKIR_002791"
                     /pseudo
     CDS             complement(26137..26289)
                     /locus_tag="ACHKIR_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415917.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation operon negative
                     regulatory protein PaaX"
     gene            26290..26496
                     /locus_tag="ACHKIR_002792"
                     /pseudo
     CDS             26290..26496
                     /locus_tag="ACHKIR_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(26668..26994)
                     /gene="ydbL"
                     /locus_tag="ACHKIR_002793"
     CDS             complement(26668..26994)
                     /gene="ydbL"
                     /locus_tag="ACHKIR_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HFO9990503.1"
                     /translation="MKRTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVAL
                     KTDAETEKLVTDINAERKASYQQLAKQNNVSVDDIAKLAGQKLVERAKPGEYVQGING
                     KWVRKF"
     gene            complement(27002..27187)
                     /gene="ynbE"
                     /locus_tag="ACHKIR_002794"
     CDS             complement(27002..27187)
                     /gene="ynbE"
                     /locus_tag="ACHKIR_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HFO9990504.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(27184..29823)
                     /gene="ydbH"
                     /locus_tag="ACHKIR_002795"
     CDS             complement(27184..29823)
                     /gene="ydbH"
                     /locus_tag="ACHKIR_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HFO9990505.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQAMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVVARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLTDPTLAFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGHLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWSE
                     DEPLLLTDVSVDVLGGNVLMKQLRMPQHDSALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNAHLPGNDCPQGKECEDKQ"
     gene            30031..31020
                     /gene="ldhA"
                     /locus_tag="ACHKIR_002796"
     CDS             30031..31020
                     /gene="ldhA"
                     /locus_tag="ACHKIR_002796"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFO9990506.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFEQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            31131..31553
                     /gene="hslJ"
                     /locus_tag="ACHKIR_002797"
     CDS             31131..31553
                     /gene="hslJ"
                     /locus_tag="ACHKIR_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HFO9990507.1"
                     /translation="MKKVAALVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(31550..31816)
                     /gene="ydbJ"
                     /locus_tag="ACHKIR_002798"
     CDS             complement(31550..31816)
                     /gene="ydbJ"
                     /locus_tag="ACHKIR_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HFO9990508.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTTIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            32090..35614
                     /gene="nifJ"
                     /locus_tag="ACHKIR_002799"
     CDS             32090..35614
                     /gene="nifJ"
                     /locus_tag="ACHKIR_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFO9990509.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFAAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPDSVRTVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSVDEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLKDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
BASE COUNT         9198 a         9293 c         8379 g         8877 t
ORIGIN      
        1 ggaattttct cgagcatagc cagagccgca gaatttgcta cggttatagt aagtcacctg
       61 tgggaaaacg ctatgcgcaa atatcgtcta agtgaagaac agcgagcctt tagttatcag
      121 gatgatggca ctaaaaaaag tgtgttatta cggcagatta tcgccatgag cgattttaac
      181 gatgtgatag caggaaccgc tggcggctgg atcgattgcg aaacggtgtt agcacaggaa
      241 ggaaattgct ggatttacga ccagaatgct attgcgttcg gcggagcggt gatttccggt
      301 aatacgcgca tcaccggcac cagcgtactg tggggagagg tttacgcaac ggataacgtc
      361 tggatcgata atagtgaaat cagtcagggt gcatatataa gtgacagtgt caccattcgt
      421 gactcattag tttgcgggca atgtagaata tttggtcacg ccttaattga tcaacattct
      481 atgatcgtcg ccgcacaagg cttaacgccc gaccatcaac tcctgctaca aatttatgat
      541 cgcgcaaggg ttagcgcctc gcgtattgtt caccaggcac aaatttatgg cgatgccgtc
      601 gtccggtatg cttttattga acatcgcgcc gaagtttttg attttgccag cgttgaaggt
      661 aatgaagaga acaacgtctg gctatgcgat tgtgctaaag tctacggtca cgcgcaggtg
      721 aaagctggca tagaagaaga tgccattccg acgattcatt acagttcgca ggtggcggaa
      781 tatgccattg tggaaggtaa ttgtgtgtta aaacatcatg tcttgattgg cggaaatgcc
      841 gtggtacgtg gcggaccgat tctgctcgac gagcacgttg taatacaagg tgagagccgt
      901 attacaggtg cggtgataat tgaaaaacat gtggagttaa ccgaccacgc cgtcgttgaa
      961 gcctttgatg gcgacacggt tcatgtccgt ggtccaaaag tcattaacgg cgaggagcgc
     1021 attacgcgaa cgccgctggc agggttattg tgaatcgata atacgcgcgt ataagttcac
     1081 atcatcatag gcatcattca ggaactcagc ctgtttcaga caaccttcaa ggataaaacc
     1141 attgcgcaaa gcgacctggt tgctttgcgc attgtccacc cgacatttga tcacgaagcg
     1201 tctaagttca ccagactgag cgtaatgatg aatcaatgcc tgcagcgcct gagaaatgat
     1261 cccctgcccc tgatgagatt cgtccagcca gtagccgatt tcagcggttt tattcagtgg
     1321 ttcaatacga ttaaacgaga taacgccgat aagttcatct tctttgaaaa tcatgaacat
     1381 tttggcatag ccgcgttgat gcaacatcac attaccctgc accgtttttc gcgtgtcctc
     1441 ttcactttga acaaactgcg gccagtttag cgactgctgt aaccaggttc tatttttaca
     1501 gattaactga taaagaggtg tgacatgact ttctgcaaca gcatgtaatt caagtgattc
     1561 acttactttt atcgtttcag tcatcttatc tccagtcaat ccatgcagtt gttaaaaaag
     1621 ccgcctggct ggcggcttag gaaagtttac acctctttga cgctcacctg ctgcaggatt
     1681 ttgagcatct catcttttaa ctgtagcttt tgttttttca tgcggaccac ttccgcattg
     1741 taccctcgac cgtcggaacc ttcctttctg gcaatttcat gatcaagttt attgtgttta
     1801 tcgaacaagg acataaagcg aggattttcg tttttcagac gggatattaa atctcggtat
     1861 tctggaaaca tacgcactcc ctgttagtgg ctgacaaggt gtatctgaaa tacacaccat
     1921 cagattactc atttaactta gggaaaaatg cgagcgggat cgtgtttaac cagacggcac
     1981 ctcccgaaga agatgccgaa gaaaaaacga ttaactcatc acgcggtcat caacatactg
     2041 acgtttatcc ggcgctggcg ggaaatactg atacagccat gtttcactga tagcgtcgcc
     2101 ctgacaacgc agatacatcc gcatatcgac cggatcagtg gagtccgaag tcggatacca
     2161 gtcaaactga atacgataac catcaatggg ttcaatatag agaatttcga tttgtttcgc
     2221 ttccccactg gaaagcgtaa tcaccggctc aatgcctttt ggcgcggcag ctttcagatc
     2281 accaccaacg aaatcgacgg caaaacgtcg cgcccatttt tcgggatagt gttcacctgg
     2341 cgcccaacct tccgggaaac cgcccatgcc ggtacgcgtc gccataacgc gcgctaatgg
     2401 gcaatgaaca ggcggttgcg cactccagta cagacgatac tggaatgcaa actcatcacc
     2461 cgcttttacc gctttttctg gctgccagaa gcagacaatg ttatccagcg tttcgcccgt
     2521 tgttgggatt tccatcaggc cgatggtgcc cttaccccac ttgttacgcg gctccaccca
     2581 cagacttggg cgtttgttat accagcccat aatgtcctga taatgggaga aatcacgatc
     2641 cagttgcaat aaaccaaacc ctttcgggtt gttgtcggtg taagcattga actgcaattt
     2701 ttgcggatta ttcagcggac gacaaatcca ctcgccattg ccccgccaca tggacaaacg
     2761 atcagagtca tggatttgcg gatgaatggt gtcgcacatc cgacgttcat tagtaccgca
     2821 gctgaacatg ctggtcatcg gcgcaatgcc cagctgttta atgtctttgc gcgcatacag
     2881 gtgattttcc acatccataa tcacctgact tttctcacaa tggatagtga acttataggc
     2941 accagtaatg ctggcgctat cgagcaacgc ataaacggta aaggtggttg cccccggttt
     3001 taccgtatca aaccagaagt cagtaaagtc ggggaactct tctttactgt cggtgtaagt
     3061 gtcgatcgcc aggccgcggg ccgacaaacc gtattgatat gtatcatcaa ccgcgcggaa
     3121 ataactcgcg ccgaggaatg atacgacatc acggcgcgcc agttcggggg ctttaaacac
     3181 gcgaaaaccg gcaaaaccga gatcactttg tccttctaat tgtttggtat caacacccgc
     3241 atcgttgtat ttgaacaact ccgggcgaaa gtgaatttca cgcgccagat gtgttgctgg
     3301 atctacagaa aacatacgaa cgcggcgacg gaatcccatt cccatatgga agaactgagc
     3361 gtccagttga cggttctcaa cgttatgcca gagcgatttt tcggcgtcgt attgaatact
     3421 gttataagcc tgcggcgtca ttgtcgccag agtgtcaggt aacggacgcg gcgcaccacg
     3481 ccacgctgtt tgcgctaagt cgtgcgccat tgactgtaga atggagaagt caaaacgttg
     3541 ggtttgcccg tcggcaatat cagaatctgc cgcgaatgcc gcctgagaaa aaagagaagc
     3601 aatgccgctg gtaccgcaca cggcggccat agccattgaa cctttaataa atcgtctacg
     3661 atccatacct gaaagtgagt ccttctggtg ttgtgaatgg tcccgcgctg atcgttttca
     3721 gcggcaagaa agtgcttatt tatgaccgct cactctagac aatatttatt aataatccaa
     3781 ttgttgaact gagagaatat gcataaatcg gcaatttttt tatgtcgatg ggatgagcct
     3841 gattcgtttg taaaatcagg caaatttttg caaaggcgga tggtcagaat ttaaagcaaa
     3901 ccacagcgac gtttactgcg ttcttccgct tcctgataaa gctggaactc atccaaaacc
     3961 gggcagccca acgcctgctc aaatgcttcg cgactggcat taccgtgact gcgtgcctgc
     4021 gtttcattac ccagatttga ctggaagatc cccgccgcgc taacaggcaa gaaatcttca
     4081 taggtaatgg gttgcgccac tacccaacca cgttcaatta agggctgtgg atcgtctccg
     4141 ggatgaatcg cctgacgatg cgtctcacct gaaggcgtca gacggtaccg gaaccatgcc
     4201 aacccttgct gacgcattaa aaactcactg tcaggaaaag tgcggaaggt ttcctgtaaa
     4261 tgcatttggt gagtgagatt atcctgcccg gttccagcgt tccgcagtag atcatcatac
     4321 agttgtcgtc ctttcggcgt taatgccacg ccacgctgtt ctatttcacc aaagcgcgcg
     4381 gtatgcgtgc cctgtttctg ccctgcaaac aacaccgtct cttccagtgc tttaaagcta
     4441 gtctggcgta gtaaaatcgg tacctcgcgg cgcggcggcc cttcgatcag aattttgggt
     4501 tcaattccgc attcaggcat catcgactgc acccggtcaa tatccagcgt gcgtggcgtc
     4561 aggtggttga tatggcatcc aggaaaacag accacatcag caatcaaccg atgttcgttg
     4621 tgcaaggcgc gataggtttc ttcatctacc gttgctgact ggtgccaacg aaacgtttcc
     4681 agggcttcct gcacaaactc ctgtgcctgt gtttcgttaa aaccgccccg ctgctcatat
     4741 tcctctaaca gttgtcgaca acgtggggtg aagatatcgc gctgacgcag aatctccgcc
     4801 gctttctggc gcaaaatttc gttctcgata agctcaaggc ggagtaatga agtaaaaacg
     4861 cggaagggat tacgcgccag agaagcatca tcaatgggcc gaaatgctgt ggaatgtacc
     4921 ggcacccccg cctgcgagag atcgtaatag ctcaccgggt acatccccat aatggcaaac
     4981 atccgccgaa gagtagcaag ctcttgtgca gtcccaacac gaatcgcccc atgacgttca
     5041 acattcagtc gcgccagctc gtctgcattt accatttttt cgtgcagttg aggattgttt
     5101 tccagcacag ccagattcac atcagctacc agttccagca gcgtaccgta ctgcggaact
     5161 tcttgctggt acatggctga cattgcctgc gaaaactgtt cccgaatctc atccgccgtg
     5221 atgctgttcg ccataatgtc tgcctccagt ggtcagtaat ctggagtgta ggtaaccgca
     5281 ttcactctgg cgggaagaat ttgcaaactg tgatctcgcc gcgaaaacat caatattatc
     5341 catttcgctg taacataatt gctttaattg ttaataatat tttgcaatca agttatcata
     5401 atcaaacaac ttcacttgtc agcgacaccg cttcgttttt aacatcgctt atggaaaaaa
     5461 atagtctgtt tagtcagcgc atccgtttgc gccaccttca tacattcgta gctgtcgcac
     5521 aacaaggaac tttggggcgc gcggctgaaa cccttaattt gagtcaacct gcgctctcta
     5581 agacattgaa tgaactggag cagctgacgg gcgctcgctt gtttgagcgt ggtcgtcagg
     5641 gggcgcaact taccttaccc ggcgaacaat ttttaacgca tgcagtcaga gtgcttgacg
     5701 ccatcaacac tgctggacag tcgcttcatc gtaaagaagg tcttaataat gatgtcgtca
     5761 gggttggtgc actaccgact gcggcactgg ggatattacc ttcggttata ggtcagtttc
     5821 atcagcaaca aaaagagacg accttgcaag ttgcgacaat gagtaaccct atgattcttg
     5881 cgggtttaaa aaccggggaa atcgatatcg gcattggtcg gatgtcagat cctgaactga
     5941 tgaccggact taattacgaa ctgctgtttc ttgaatcgct gaagctggtt gtccgcccta
     6001 atcatccgct acttcaggag aacgtaacgc taagccgggt gctggaatgg ccggtcgttg
     6061 tatcaccaga aggcactgcg ccacgccagc attcagatgc attagtgcag agccagggat
     6121 gtaaaattcc ttcgggttgt atcgaaacgc tgtctgcttc gctatctcgt caacttacgg
     6181 ttgaatacga ttacgtgtgg tttgtccctt ctggcgctgt aaaagacgac ctgcgtcatg
     6241 ccacgctggt ggccctgcct gttccgggtc atggtgcagg cgaaccgatt ggaatactga
     6301 cccgcgtaga tgcgacgttc tcttctggtt gccagttgat gattaacgct attcgaaaat
     6361 caatgccgtt ctgaaaggtg aagggatctg tcgatccctc ctttaacatt ttcacactgt
     6421 agcgaaacta actggttcac cagctccgcg aggttctgcc gacacagaat gtttgttcag
     6481 acggaatacg tccaccgcct cagttaatcg tgccgcctgt tcttcaaggg acactgccgc
     6541 tgctgaggcc tcttctacca gagaagcatt ctgttgcgtc accttatcca tttcagatat
     6601 cgcctggcta acctgcgtta tacccctact ttgctcatcc gaggcggcgg caatttcctg
     6661 catgatatgt gtgacactcg cgacggcatc aacaatagtg ctcatggttt tcccggccgt
     6721 ggccacctca tccgatccca ggtcaattaa cctgactgat tcactgatca agccttcaat
     6781 ctctttcgcc gcctgagcgc tgcggcttgc gagtgtccgt acttcgctgg cgacaacggc
     6841 aaatccacgc ccttgctcac ccgctcgcgc ggcttcaacg gcagcattca gtgccagaat
     6901 attcgtctgg aaagcaatac tgttgatgac ggcggtgatc tcagaaattt tcttcgaact
     6961 cgtggagata gcgcccatcg tttttactac accggaaacc gtctgcccgc catcgctggc
     7021 tttaatagaa gcctcttgcg ccagtttgct ggcatgatgc gcgttatccg cattctgttt
     7081 caccgtcgca gtgagttgct ccatgctggc ggcagtttgt tcgattgccg ccgcttgttc
     7141 ttcggtgcga gatgaaaggt ccgcattgcc agcggaaatt tcgctggtgc cacgataaat
     7201 ctcttccgca ccctgtcgaa cagtccctac tgtcatcccc agtgaatgct gcatttgctg
     7261 taaatgacga cttaagcgac cgatttcatt acgacccgcc ggttcatcat tcatcgtcag
     7321 atcgccactg gcgatttttt caatccgttg tgcggcatgt tgcagtggac gaatgacgat
     7381 ccgacgtagc accataaatg ttatcagcgt catgaccagg gcaagcacaa acgcgccaat
     7441 catgaacatc ccacccaggc gggtgcgctg atgggccagt tccgccagtt ggttggctct
     7501 ggtgctacgt atcttaacgg ctttgctcaa aatatcggta taagcattat ccagcggtcg
     7561 gatctgctca ctttcatgat tgataatcgc ttcaaacatg ccatttttgg catatttcaa
     7621 cataggttgc atacccgtga tataagcctg gaagcgttga tttaattcag tgtcgagggc
     7681 ttcatcagca ggtgttttca ccgaccgatt ctgataagca cgataacctt gctgcgactg
     7741 tttaatctcc gattcggctt gcgcaatatt tcgcttcatt gcttccattt ccgcaatacg
     7801 actcgctgcc ccggcctgaa tcatattgat ccgggctgaa cgcaaaaaat ctgaactgtt
     7861 cgctaacccg gtccgaatct caatctccgc agtaacatca cgctgatcgc gatcggcctg
     7921 ccagaggaaa tagccagcca gagcagaact taatgcgaat agaactaaga taccgccaag
     7981 aatgcaggca aataacggaa ccagcctgat gtgatgcaaa aaacctaatc gctgtgaggg
     8041 agttgtattc attaccgttg tctctcgtcc aggtttactc ctgatagaaa gtcatcggca
     8101 cttgacggta attacttatg accaaaaacc tctttgcgtc acatttttac aacaattatg
     8161 gcagagaaga ataatgccat tggaatgaag agagtaacga atgattaaac tggctaaccc
     8221 gaatgacgaa aaattctgga aaacagcacg caatttcgtc aggtgattat tttcgtccct
     8281 aaggaaacca cataggatgc tcacgggtgc ctgagacttt ctgttttcag gctaaccggg
     8341 aatcagaaag acgaaggccc cgaccgatat ttctatcaac cgaggccaac gaatgaacac
     8401 agcaattcag atcttagcct cttacccgcc atccggcaag gagaaaggct atgaagcgca
     8461 accctctggt ggtgtgtctg ctcattatct gcattacgat tctgacattc acactcctga
     8521 cccgacaaac gctctacgaa ctgcggttcc gggacggtga taaggaggtt gctgcgctca
     8581 tggcctgcac gtccaggtaa gggcaagcgc ggggattttc cccgcgcatc tgactagcag
     8641 attcattttc tcatggcacc ccttttacac ttcagttaat gcatgttttt ttcgatgcac
     8701 taatagaacc atcattacaa acaaatttgc cgcctgccgt acagtgtgag atcccccctt
     8761 tcgaaccaga acagggtttg cgcccggcat cggcataaaa ggaaacaagc gttcctataa
     8821 ccaagataag tgccagtttt ttcatataac ctccatggtt tatcgaatct tatgttcgat
     8881 tttaacgtta tacgctggtt tattttgata ccgttgatga gatcaaagat cgggtgaatt
     8941 gattaaaaat aagccagatg gacgtatggg caaggattat ggttttattt gtcatacaaa
     9001 taaatataat aggcgcttcc gatgtagacc cgtattcttc gcctgtacca cgggtcggtt
     9061 ttagtacagg cgttttcttt aaatatcctt caggaacgtt tacgcggcat catgcgtaga
     9121 agtgtgttgt ccttccagaa atagtggtgt gccagtgcag ccgcagcgtg caacccgatg
     9181 acaaaatatc ccagattcgc cagcatttca tgccacgact ttaagctatc tacccgttcg
     9241 aaattggcct ctgaagcgta aggcatcgtc aaaccaaacg caaaccacgg gttgccccgg
     9301 ttatacatca tcaccaaacc aatcactggc agcgcaataa acaggagata aatcaccaaa
     9361 tggcccaaat gcgccagtcc cgtcatcatc ggttttggct taggtataat cggcggggtt
     9421 gggtatttca gtcttaacaa aagacgaacc accatcagta cgagaattga gatgccacag
     9481 gaaacatgaa tcatgttgat gaggggccga tcgctacgtg ggaagaaacc acgaaactcc
     9541 attgcgcaat acgctacgat aaccagcaaa aagaccagcc agtgaatgct gatttgtaac
     9601 cttgagtatt tatttcccat aacatttcct gctttaacat aatttgccgt taacataacg
     9661 ggcttttctt aaaatttcat taaatattgt tcacccgttt tcagtacgtt acgtttcact
     9721 gcaagaatgt agattgccca tcccgccatc ctggtctaag cctggaaagg atcaattttc
     9781 atccgaacgt tcctgacagg agaagaccat gagtaaagtt ggtattaatg gttttggtcg
     9841 tatcggtcga ctggtgttgc gtcgattact tgaagtcaaa agcaacatag acgttgtcgc
     9901 tattaatgat ctcacttccc caaaaattct cgcctacctg ctgaaacatg attcaaacta
     9961 cggaccgttc ccctggagcg ttgattttac ggaagattca cttatcgttg atggaaaaag
    10021 catcgcggtt tacgccgaaa aagaggctaa aaatattccg tggaaagcga aaggggcaga
    10081 aattattgtc gaatgtactg gcttttatac ctccgccgag aaatcgcagg cgcatcttga
    10141 tgctggtgcg aagaaggtgt tgatttccgc ccctgccggt gaaatgaaaa ctatcgttta
    10201 taacgtcaat gacgacactc tggatggcaa cgacaccatt gtttccgtgg cgtcatgcac
    10261 cactaactgc cttgcgccga tggccaaagc cttgcatgac agtttcggga tagaagtcgg
    10321 cacgatgacg accattcatg cctataccgg cacccagtca ctggtggatg gtccgcgtgg
    10381 gaaggattta cgagcttcac gcgcagcggc agaaaatatc attccccaca ctacaggtgc
    10441 ggcaaaagcc attggtctgg tgatcccgga actgagcggc aaactgaaag gtcatgcgca
    10501 acgcgtgccg gtgaaaacag gttcggtcac tgagctggtg tccattctcg caaaaaaagt
    10561 gactgccgaa gaggtgaata acgcacttaa acaagcaacc accaataacg agtcatttgg
    10621 ttataccgat gaagaaatag tctcttccga tatcattggc agccatttcg ggtcggtgtt
    10681 tgatgccacg caaacggaaa ttaccgccgt gggcgattta caactggtga aaacggtcgc
    10741 ctggtacgat aacgaatatg gcttcgtcac acaactcatt cgcaccctcg aaaaattcgc
    10801 taaactctga cgcacacagg cggaggaaaa acctccgcct cttccactca ttaagactgt
    10861 aaataaacca cctgggtctg cagatattca tgcaagccat gtttgccatc tgcgccgcca
    10921 ataccagatt tacgccatcc ggcgtggaat ccttgcatag cttcgaagtt ttcacggttg
    10981 atgtaagttt caccaaactt cagcccttta atggctttca tcgcgacgtt cagattttgg
    11041 gtatagattg atgaggtcag gccgtaatca ctatcattcg ccattgagat agcctcttcc
    11101 agcgtgtcaa atgcgaccac cggcagaacc gggccaaagg tttcctcatg cataatcgac
    11161 atttcctggc gaacatccag cagcaatgtc ggcggataat aatatccttt cccctctacc
    11221 gctttgccac ccaacgccac tctcgcccct tcttctactg cgcgcgccac tttttgctcg
    11281 accctttcca gcgccgcggc gttaatcaac ggccccatcg caatgtcgtt gcgttcagcg
    11341 gggttaccaa attgaaccgc ctgcatcgct tcacccagcc gattgacaaa ctgatcataa
    11401 atgcctttct gtacataaac acgttctgca cagttacaca cttgcccact attaatgacg
    11461 cgtgaatcaa cgatggcttt gactgccagt tcaagatcgg catcgtccat tacgatagcc
    11521 ggtgctttac cccccaattc caggcacact ttggtgatgt ttttcgccgc agtcgccata
    11581 atcttttcac ctgcagagac gctgcctgtc atactgacca ttgcgacctt tgggttaccc
    11641 gccagttctt gcccaacggt ttcaccacgc cccagaacaa ggttaaacac gccgcgcgga
    11701 aggcctattt catcgacaat tttggcgaat gcaatcgcat tgtttggcgt aaattcactg
    11761 ggtttaatga cgatggtatt accggtcaaa agagcgggag ccattttgcg ggcaatgagg
    11821 aagaacggga agttccacgg cagaatgccg gtagtcacgc caagcgcacg tttaaacaga
    11881 agaatatttt ctcctggacg atcgctttga ataatctcgc cttcataacg ccgtgcccac
    11941 tccgccatgt aatcgatata gtccgcagta aaagccactt cgacttcagc cagctgctgg
    12001 atcttacccc cttcttcaac aatcagcgta ctgatttcac tggcgcgttc gcggatcccg
    12061 gcggagattt tgcgcaacca actggcgcgt tcaatagcag gcaacgcttc ccattctggt
    12121 tgtgcacgtt ctgctgcatc gattgcctta cgggcatcct cggcctgacc atcgggaatg
    12181 cgggaaatga cagcctctgt agcagggttt accacatcaa tccatgcgtc tccacgccag
    12241 gtaacaaact gtccatcaat atacatagga tgttgaacgg gtactgacat gagcgactcc
    12301 tgtgatttat atgtttgttt ttggtaaatg tattgttaat taacatcaat catcatagag
    12361 acggcatcat gaaaggacaa ttctgtgaag ggcttcataa aaacaagtat aaaaggaata
    12421 agaggcagaa ataaaaggca ggagttatgc ccgtttgcaa acaagtggtt cacaaacggg
    12481 caatttttaa cgtaacgagc gactttccat cgcctcgatt agcaccgcat cgtgtttcag
    12541 cgtttgccat gcatggatga cttctgccgg aaaatcaacg tgaacgataa aatctcgccc
    12601 gcggggaccg tagccatcgc tatcatcgcg taaacgcggc agctcgccag tgagtagtga
    12661 gagataacgc tcaaccggcc ataatccttg caacggatta acaaagatta cactgtctgc
    12721 gttatttcct aactgaggaa cgaatccggg ataacttaac cagcgtggtg catcgggatt
    12781 gtccccagtc atacggcgga acgtcgccat cgtgcgccgc tgcatggtgg ggtcctgaac
    12841 aacgatagcc gtatgaactc gttctacggc ctgattcaat agcgcgatgc taaagcgtgc
    12901 gttttcaccg cagtttgttg actggtcttc aatccagatt ttttcatgcg gaatgtgcca
    12961 gaactgatga gcgatatccg ccaggatggt cgcttctgct ctgccagtgg tgcggatagt
    13021 gttgtagtgc ggatgctgtg cgatggcgct atacaaaaaa gttgtcgagt gaccgatacc
    13081 accactaatc agtagaggaa tttgctgatc gcgagcaatc ttacatgccg cgtcgatagt
    13141 cggcataacc gcattgcctg caaggatcac gcaatcggcc tgatacggca tctcaccgga
    13201 gaaatcatcc tgcgccagcc actgaccgat aacatttatg gcatctatgg ttgccggcga
    13261 aagcgtcgga aacggggtaa tgttcatggt tcctccttat cgttccctta agaatactgc
    13321 gtgagataat agtgagaagc ggagcactct gaaaggagga agtcgtattt ccttaaccgc
    13381 cactccgcaa gcctgacata tcttttaagg aaatgctgta gataaacatt attcatcttg
    13441 cagctgagaa ttgtattttt ctgaattcct gaatttttgc ccttaccgtt ttagaattca
    13501 taaaaaaccc ggcgctaaat gcttaccggg ttttagattt atcaggcaaa tagccgcgtt
    13561 aaccgctaat ctgttccatc gcctgcaaaa tacgcttatc tgaaatcgga taaggcgtac
    13621 caagttgttg agcgaagtaa ctaacgcgca actcttctat catccaacgg atctctttca
    13681 cgtcttcatc ctcacgacgt gcgggcggca gtttgttgat ccattgctgc cacgcctgct
    13741 ggacattttc gactttcagc atctgagcac ggtcacgatg cggatcaacc gccagttttt
    13801 ccagtcgttt ttcaatcgcc tgcaaataac gcagcgtgtc gcccagccgt ttaaaaccgt
    13861 taccagtgac aaaaccgcga tacaccagcc cgcccatctg cgctttgatg tcagaaagcc
    13921 ccagcgccat agtcatatcc acccgccctt tcaggcgttt gttgatattg aacactgccg
    13981 taaggatttg ctcgacctgc ttcgcaatat ccaccaccgt atcgttcagt tcagcgcgga
    14041 ctttctcatg cagcgcagca aagccttctt ccgtccagac cgggcctcca ttggcgtcga
    14101 tcagttgatc caccccgcag gagatacagt cgtcgatcag ctccagcact ttgccatacg
    14161 ggttaaagta cagccccagc ttggctttgt tcggtaactt ttcatgcaaa tacttgatcg
    14221 gcgatggaat attcagcagc agtaaacgac gaagaccgtt ccacatcgct tgcttttgtt
    14281 ccagcgggtt atcaaacagt ttgatcgcca cgctgtcgcg ctcatccacc aacgctggcc
    14341 acgccttcac tttgtagttg ccacgcttct gttcgtagct ttccggcagt tgcccaaagc
    14401 tccagatatg taagccgctc tgctcgatac cgtcatccgc caccgcagac agcgtttcct
    14461 gcactttgcc tttcagagca tctttcagat cctgtaatga gcgcccttct tttagcttct
    14521 tgtttttgtc atccaccaca cggaaggtga ttttcaggtg atcgggcacc tgatcccagt
    14581 gccagtcttc gcggtcaacg gtgacgccag tcatccgccg tagctctcgt tcaagactgt
    14641 cgagcaacga caattccaac ggtgtgacgc ggcctagaaa cgcttcggcg tagtttggcg
    14701 cgggcacaaa attacggcgt actggtttcg gcaacgattt aatcagagca atcaccagtt
    14761 cgcggcgcag accggggatt tgccattcaa acccgctctc ttctacctga ttaagcagcg
    14821 gcagcggaat atgcacggtc acaccgtccg cgtccgcgcc tggctcaaac tgatagctca
    14881 aacgcagctt gagattgccc tgatgccaga agttcggata atccagcttg ctgatttttt
    14941 ccgccccttc tttgatcaac atgctctttt caaagttgag caaatcaggc gtttcgcggc
    15001 tgactttttt ccaccagcta tcgaagtgac gagcggagat tacatcgtgg ctgatgcgct
    15061 ggtcgtagaa ctcaaacagc gtttcgtcat caaccagaat atcgcggcga cgtgatttgt
    15121 gttccagctc ttccacttcg gcccgcagtt tcaggttttc acggaagaat gcgtgacgcg
    15181 tctgccagtc accttccacc agagcgtggc gaataaagag ttcacgacat aacgccggat
    15241 cgatctggct gtagttgacc ttacgcgcag caacaatcgg caaaccataa acagtgactt
    15301 tttccgttgc catcaccgcg ccctgcgccc gttcccagtg cggttcgctg taggtgcgtt
    15361 taatcaaatg ctgagcaacg ggttctaccc attcagggtc gatacgcgca gcaatgcgcc
    15421 cccacaggcg gctggtttct accagttccg ccaccattac ccatttcggc ggctttttga
    15481 ataaaccaga accgggaaag atggaaaaac gcgcgttacg tgcgccggta tattcttgtt
    15541 tatcggcatc tttcatgccg atatgggaaa gcaaacccgt cagcaacgca atgtgaattt
    15601 cgcgatactc cgccggttcg ctgttaaccg gaatgccaag ttctttcacc acctggcgca
    15661 actgggtgta gatatcctgc cattcgcgca cgcgcagata gttgagataa tcggtacgac
    15721 acaggcgacg gaaggcgtta gaagaaagcg ccttttgctg ctcgccgaga taattccaca
    15781 gatttacaaa cgcgaggaag tcggattctt tgtcgtggaa gcggcgatgt ttttcgtctg
    15841 atgcctgctg tttatccatc ggacgctcgc gcggatcctg aatggagagc gcggacgtga
    15901 taatcatcgc ctcacgcacg cagccatgtt tttgcgcttc cagcaccata cgcgccagac
    15961 gaggatcgac aggcaattgc gaaagctggc gaccgagcgg cgtcagttta taggcgctgg
    16021 cctgttcatc agtggtgatc gcgcccagct cttcgagcag acgcacgcca tcctggatat
    16081 tacgtttatc cggtgcttcg acaaatggga acgcggcgat atcgcccaac cccagcgcgg
    16141 tcatctgcaa aataaccgag gccaggttag tacgcagaat ctccggatcg gtaaactccg
    16201 ggcgcgagag gaaatcgtct tcggaataga gacgaataca gatcccttcg gacacacgac
    16261 cacagcggcc tttacgctga ttggcagacg cctgagaaat cggctcaatc ggcaaacgct
    16321 gcactttagt gcgatagctg tagcggctga tacgcgccgt acctgggtcg ataacgtatt
    16381 taatacccgg tacggtcagc gacgtttccg cgacgttggt cgccagcaca atgcgccgtc
    16441 cactgtgcga ctggaacacg cggttctgtt cgctgttcga aagccgcgca taaagcggca
    16501 agatttcggt atggcgtaag ttcagcttgt tcagcgcatc ggcggtatcg cgaatttccc
    16561 gctcaccact cataaagatc agaatgtcgc cagggctttc ctggctcagt tcgtctacgg
    16621 cgtcaaaaat cgcctgcagc tgatcgcgct cggtgtcatc ggcttcttca acaatcggtc
    16681 gatagcgcac ttccaccgga taggtccgac cggagacttc gataatcggc gcattattaa
    16741 agtggcgcga aaagcgttcc gggtcgatag tcgcggaagt gataatgatt tttaggtcag
    16801 gacgccgcgg tagcaactct ttcaaatagc cgagcagaaa atcgatattc aggctacgtt
    16861 cgtgcgcttc gtcaataatg atagtgtcgt actgcatcag caggcggtct tgctggatct
    16921 ccgccagcag gataccgtcg gtcatcagct tgaccatcgt gttatcacta acgtgatcac
    16981 tgaaacgcac tttgtaaccg atgcaaccgc ccggctccgt tttcagctct tccgcaatac
    17041 ggttcgccac cgttcgtgcc gccaggcgac gcggttgggt atgaccgatc agccctttaa
    17101 tcccgcgccc cagttccata cagattttcg gtagctgagt cgttttaccg gaaccggttt
    17161 ccccggcgac gatcaccacc tggtgatcac gaatcgcttc gagtatgtcc tgctttttct
    17221 gactaaccgg caaattgtcc ggataagtga tttccggtcg tgccgcttca cgcagcagga
    17281 ctttccctgc cgcatggtca atctctttcg ccatctcctg gaaaatggcc tgttgtgcat
    17341 caggattttt aaccttcttc acgccgtgca gacggcgaga aaaacgcagt ctgtcgcgca
    17401 gcatcagcga atccagccgc tgctgcaagg ccgtaaaggt caatttttgt tgttctgtca
    17461 taacgttagt gggcagtagt ctgcctgatt aattttcttt taaatgatag atatagagta
    17521 ccacatcgcg gctttctatg cgttgttcaa taaattcgaa cataggcttc gatttattgc
    17581 gctatctctg tggcaggatt gtgaataaag tgtcaacaag caacggggca tcgcccattc
    17641 aaacatctat aaggaaacac catgagcaag gtattagttc ttaaatccag catcctggca
    17701 gggtactctc agtctaatca gttgtccgat tattttgttg aacaatggcg cgaaaagcac
    17761 tccgctgatg aaatcaccgt tcgcgacctg gctgcaaatc cgattccggt gctggatggc
    17821 gaactggttg gtgcgctgcg tccgagcgat gcaccgctga ctccgcgtca gcaggaagct
    17881 ctggcacttt ccgatgagct gattgccgag ttgaaagccc acgacgttat cgttattgcg
    17941 gcaccgatgt ataacttcaa catctcgact cagttgaaaa actattttga cctggttgca
    18001 cgcgcaggcg ttactttccg ctatacggag aacggtccgg aaggcctggt caccggtaaa
    18061 aaagccatcg ttatcaccag ccgcggtggg atccataaag atggcccaac ggatctggtg
    18121 acgccgtatc tgtccacatt cctgggcttt atcggcatta ccgatgtgaa atttgtcttc
    18181 gccgaaggga tcgcatacgg tcctgaaatg gcagcgaaag cgcagtctga cgcgaaagca
    18241 gccatcgaca gtattgttgc tgaataactg gacacgatct tgatgtggac tgtagatatt
    18301 cagtccacat ctcaatccgc ttacctgttt tcccataatt tcagcatcgc tttgtgctct
    18361 tccttaagca caatccgcga gcgtctggct cgaatataac taatcgcttc atcaacggtt
    18421 ttacaatgtc cgtaacataa cagccatgcc gccaccacca gcgcactgcg cgataatccc
    18481 aacgcgcaat ggaccagaac gctgccttgc tcttcgcgta atgtttccag catcgccacg
    18541 gcctgtcgga gctccccctc ttccggaacc accagatcca gcatcggtac acaaaaatag
    18601 agttgatctt ttgtggctcg tccgcgaggg aattcaaagg tgacgtccag aacagcattc
    18661 tgtgccggaa tatgttgtgg aaacgcccct aaataaacac cagcagtaat tttgctcacc
    18721 gactccaggc gaagacaaaa ccaacgcata gacagccaca tcccgatgcg ccagggcaat
    18781 gtcagccagt aaacggcggg cgggagtttc ccctaactat ctttccctgt tgttatcgcg
    18841 ccaagcccac cgtacccacg gccaatgatg aatagcgata atactggcca acataaccag
    18901 actgaccacc ataactgaag cattatcatt agctccatca acacaatgca cgagcacgcg
    18961 cctacgacat atggcagtgc tattttgatt cgacgttgat caggtttctg ataattccaa
    19021 cgacggtcga cgggcaccat ccagtcaatt aacataccta ccgccagccc tgtgatgaca
    19081 tcaataaaat gatgctgcca ggtcgtcagc gtcgaaatgg cgatgagtaa aaaccatccg
    19141 ccgcagactt tacgccacct cacagccaga tgctgacgaa agtgacgcca aagtagccag
    19201 cagagaataa tatgcagcga aggagactgg ttataaggca gatcaaacaa ttcaagttgc
    19261 gaaaataacc atcctgtcac cccactcact tcaggacgga taaaactaaa cttcagcgga
    19321 tagagaaaaa aaccgcagca ggccattacc gttgccagaa taagccggtg gaccagacgg
    19381 cgctgctcaa atgtcgagct acaaacaaac agcgaaaatc catataaaag atccagactc
    19441 cagtaaggaa caatagtcca gggaagaaaa gggatcgccg tttcccaacc gaataactga
    19501 ctggggatat catggttgtt aagatcctga gtcgcggtga actgattaag aaatccatat
    19561 gtgaagaaga aaaacggggc cagcaacaat aaccagccag cgccttgtag caatacgttc
    19621 cggcgttcag ttaccacgac gcaccgccat cgaaaccgta aaaatgcccc actcatcaat
    19681 ccgttgtgtg catttatcga atccggcatc atgcacgagc gaatccatct ccccttgaga
    19741 acgcacgcgc attacccacg gtttaccatc tttatgactg gttaacaccc cggcaatcag
    19801 ctccagttgt gggtgccacg gctgcccggt gtagatgaga atgccacccg gatcgatggc
    19861 attggcaaga cctgcgagtg agttttttac ctgctcattt tcgggaaaaa gctcatacag
    19921 tccagagaca atcgccagcg taggccgcgg agttaacgcg ctgagctcct ccgggttaaa
    19981 cgcatcgccc tgttcaaaac gcacccgccc agacattccc cgttgggcaa tcatctcttg
    20041 cccctgtgca acatttaact cactgtaatc acgtaagaaa atatcgctta cggcaggctc
    20101 gttcgccagc gcatccagta catagcgtcc atgccctgcg gcaatgtcaa ccacgcggac
    20161 ggctaaacct ttagcgtgga gatcggcaac ggcttgttta atcagcattt gtaaatgggt
    20221 tttgcgctgg cgaataccgc gccagccaac actgttcagg tagattttgt cgactaaacg
    20281 cccgaatgcg ttactacctt gcggttgatt ttgatagaca taatccagcg aactgcctga
    20341 atcaaagccg gtgctcattc cgagatgcag gcccgcagag tgcgccccga acaatttcat
    20401 cgctttacgc ataaagcgat acgccaaatc aaccggcgat aatggcacgg gtccaccaga
    20461 aagtagccgc catcgatccg ctgatggtcc tgtgcagtct tcatgctgat aatctaattt
    20521 ttgcgatttg ttagcatata agtgactaat aaagctttgc attttttcaa ataccagcgc
    20581 cctgttctct tcacccaacg tgtcgtgata aaagcctggc agcagatgca gctctttcag
    20641 agggctacgt aaacgctgat aaaaatcaat ctgcggttgg cggtgcacca catagtcatc
    20701 gcctgatatc agaagttgcg tggggagcgt aatcgccgcc gcatcgctaa taatacgttc
    20761 agacgtttta tagagatcta gcaagatatt gacggcaatc gcccttgtga tcagcggatc
    20821 attattgaaa ctagccaccc gttgccgatc gtgggtcaaa tagcgtcctt tcacatagga
    20881 attaataaaa aacagaccac gcagacgatg ccataacgcc agcgcaggac gtgccagcgg
    20941 cacatacaat ttaaccttaa aggccggaga agccagcacc agcccgcgaa tagcaggcgc
    21001 ataatcatga acccatgtag caaccatcac tgcgccgacg ctttgcgcga tcacaaccac
    21061 ctcttccagt ccgacctggc tgtcgctggc agcaaaacgg acaaattcat cgacatcttg
    21121 cactgaactc gcaagagacg gactataacc acgcggcccc gaagtttgtc catgccctcg
    21181 ggcatcccat gcataaaaag cagtatctgg catcgccagt tcatcaacga gatgttgtag
    21241 acgaccagaa tgttcatgac cgcgatgaaa taagacgatg acctttttcg cccctggctg
    21301 taaagtgggc cagtgccgat aaaacaacgc tgtattatca ctggtggtaa aaaaatgttc
    21361 cccagggatg cgtgaatttt ccattctctc tattttcctt cagtaacagc aatagcgaat
    21421 aaaataaaaa aataccggag cggtgaaaat taatgagtca atcctgtcaa ggagtccgcc
    21481 atgtcctggt aatagttttc cactatcttt aacaccaaca tctcgtttga tggctgacat
    21541 cactacatcg ccgcaaaaac cactaatacc aattaacaaa cccgctaata atgcctgtaa
    21601 tgtatctagc ggtgtcagta acggtccaat aatcagtgac acaatcatgg tagtgattac
    21661 gccccccacc agaccttcca gcgtttttcc cgggctgact ttggggacca cttttcttct
    21721 gccgcaggat ttcccccata aatactgtgc aatatcgttt gactcggtta atgccaaaag
    21781 aaataacacc agtaacgcct cgccctggat atttgtggtt ggcaacatta ataaccaggc
    21841 ggcatgactc aaggcgaaaa cggtcattat ccagccacaa aaaatggcgc tcacggtatg
    21901 cagaaaaccg gagggttcac ccacaaaaac tcgccaggtg gctaatatca gaaacccaaa
    21961 caaaggtata aataacagaa acagctcaaa gcaatttaaa ccgataagca aatagttaag
    22021 aggaataacc caatataacc aacgcggaaa atgtacggat ataagcgtac aatattcttt
    22081 caatgccaga aaacttatca gcgcaaaaaa cgtcaacgtc acccagcgtg gacctgaaat
    22141 caccagtgaa aaacaaatga cgatgcccca ccaggtacga atacgtaatg taacttcatc
    22201 cccttttctc tccggtaacc gccataccag aaaacggttt atcagcgttg ctaaaattaa
    22261 caacgcgaaa agtgttgcca gagatttttc cagcattatt tttcagcgtt catcaggagc
    22321 acactccgac aacgattaat cgcagtccag agaagcagaa ttgaagcaat gctccataaa
    22381 agattattcc attgcatcca ctgtggataa atggcaacgg ccagcaccca caacccaaat
    22441 attaacgcgc gatcgctttt gccaaatgga ccggcataac tgcgaatacc gttaatcgtc
    22501 tgcgcgagta aaccgcaaaa ctcagtcaat atggtgcaaa ataacatgag tattacgagt
    22561 gatgcgttac tttccggtaa aaataaaaac ggtaaataaa gcgcaatatc ggaaataaca
    22621 tcgccagttt cattcaaaat cgccccaaga cgtgtttgct gattgcactc acgcgccatc
    22681 atgccatcca gcgcattgag tgccatacgg acaaaaagca cgatgggcaa tagcagaaac
    22741 atgccatgat atggcaccaa cgccaacaac aaaccggtga gaagagaaag tgccaacgcg
    22801 gcaagggtga tctgatttgc agacacttgc cgcttatgaa gccaaaacat cgtcggcctt
    22861 aacagtgact gaaagagcgg ttttatctgg tagagcgtca ttatcaatcc ttgataattc
    22921 tgtttgcgct atggtgcatc aattgccctg gcagagatat cgcaacacgc cgcttcatta
    22981 aaaattcaga atatatcaag gtattggggc tcactgtcag gaatgattat ccccaattaa
    23041 tcaaatagtt aaaaatatta cgctgttatt caatgcaact catcaagttt tctcatcgct
    23101 tgttcccgta ttgcgcgcgt cttaccgtta agcgccagct ggcggactaa atccagttca
    23161 gggttaacaa gctctaacgc cagcaaagga tcacgtttca gcctcgcctt taatacatca
    23221 actggaaaac ggggattatt catcgccaca atccaccact cgggatcaat ttctgccgcc
    23281 agtactgcag gcggtgtgtg gcggttgtta agtgcgccga acttgaaaaa tgtgagattg
    23341 cttttcgcca gtgtactcca ccatatacga tccggttgtt ttgccagtac atccagttgt
    23401 gctaacgcat cgggttctag tgcataataa acacgtaacg cttcgttaac ctgcgcacgg
    23461 gaactgtttt cctgcctcag aaaaaacttc gcccaaatat catttcgtgg cgtgttttgc
    23521 tgtaattcct ttaagccttg ggagagttcg cattatggca agatcaaatg tcgcaatgtc
    23581 tcgtccttca aatgcagcaa cgttgccttc atccagcatt tgcaattcct gcaataattc
    23641 aggagaagac ttagggcgaa acagcgcttc ttttttcatc tgattacaga cattccctgc
    23701 tatgcgaata ttgcttcgct taaaaatttc tttcagagca tcttcatcat ttattaggcc
    23761 ccaataatat gcatcaggat ttattatcat cagcgctttt ttgacatttt cagtgtcatt
    23821 tttgtcactc gcacggacca tgaattcact atttgtcgaa gatgcaacaa caggtgcaat
    23881 tatgtcattg ataaatatcc cgcgcgcatt aatacttccc ttaatcgtag acggattatc
    23941 gtaatatgag tcgataaata ccagtccact tgcatttatc gaaccggtaa ttaacagtgg
    24001 tgaatccgta actatattcg ttgccgttac gttacgttac gttacgttac gttacgttac
    24061 gttacgttac cgagaataac ccatggatta gaaagagaga aagtggaaat attacctttg
    24121 actgtcaaat tgccaagaac aatcaatttt tgattactct ctatattacc ttctaccatt
    24181 aaatcgccac gatgcacttt ccagtccgat gcagataaac cgacggggac aagattcacg
    24241 ctactgttga tagcttgttc aacttgttgt ttatcttcag cggtagccgc agaaaaaaat
    24301 ggttcaatat caactgcatt tacaacacat cgaagttccg cctgtactgg aagactcagc
    24361 ataaacaggg gaagtaaacc tatccagctg caaatcctgg gcttaaacat atgcactcca
    24421 tgccattaaa aaagaaacaa aagggaaatg catctttccc ttttgtatat tcgttattct
    24481 cgcgaaatac cgtccaacgt agacaacact tcctcagaaa gatgcaattt ttcagcagcc
    24541 atattctccc gtaaatgcgc aacagaagac gtccctggga tcagcaaaat attcggtgaa
    24601 cgctgtaaca gccacgccag cgccacctgc attggtgttg cgcccaggct cgctgctaca
    24661 tccgaaagtg tggaggattg cagcggcgta aagcccccaa gcggaaagaa cggcacgtag
    24721 gcaatgccgt cacgagccaa agcatcaatc attgcgtcat cagcacgatg agcaatgttg
    24781 tattcgttct gcacacagac gatttcggta atcttacgcg cctctgcaac ctgcgtcggt
    24841 gtaacgttgc tcagaccaat atgttttacc aggccttgtt gttgcatctc tgccagcacg
    24901 gtcaggctgg cttcaatcga tccttccgct gggccatgac catcccccat cataacgcgc
    24961 aggttaacca catccagtgc gcccagcccg agattacgta gattatcgtg caccgctttt
    25021 tgcagctctg ccggagaaaa tgcgggcaac caggatgcgt cctctccacg ccgcgcacca
    25081 attttagtga caattgtcag gtcgtcagag taaggataaa gcgcttcgcg gataatctga
    25141 ttggtgacgt gcggaccata aaagtcgctg gtatcaatat gattgacgcc caatgccagc
    25201 gcctcacgca gcacggttat agcgacgtgg cgatctcttg gggggccaaa aaccccagga
    25261 cctgccagtt gcatcgcgcc ataaccaaga cggttaacgg attttgtacc gagagtaaat
    25321 gtattgctgc tcatcgcacc ctgccctgta ataaaacgga ttaagaaaaa tagtatagaa
    25381 acgaaaagag ataaataatg cgaaggcgta aacaagatta ctggatgagt tgctctattg
    25441 ccctgaaaga caccaaaaca aaacgcgtca agtaccgcat ccggcaataa aaaaaccgca
    25501 acctgtatca gcgttgcggt ttaggtatca catgctaaaa attagaacgt ccagcatcag
    25561 gacgttactg tttcggtcgc agattctcat caaataccag gcgctgcctg ccaggttcaa
    25621 cttcccgcaa tggctcaact tgatgcaacg tctgcttact gcgtgtcacc agcacctgat
    25681 actcatgcgt gccctgcttt ttccatgcca gttcctgctc actaagttca cgaatcgctt
    25741 ttgccgggct gccgacaatc aggtaattag caggcatttc tgctttggct ttcacaaatg
    25801 cggaagcgcc aacaatgctg ttctcgccaa tcaccgcacc gtccatcact accgcgttca
    25861 tacccactaa tgcattgcgg tggataatgc agccgtgaag gatagcgcta tgaccaatat
    25921 gcccatcttc ttccacgaca gtatcctgat cgggaaaacc gtgcataacg caattatcct
    25981 gaatgttcgc gccatctttc accacgatac gaccgaaatc gccacgcagg ctggtatttg
    26041 gcccaacgta cacgcccttg ccgagaataa catcgccgat caataccgct gtcggatgga
    26101 caaaactctc ttctggcaca aactgagtca gaccgtctat ctgataaatt ggcataacgc
    26161 ctcctgttca atattcaagc cgccaaaacg ttgaaaatac aggcttcccg gcgcaggcaa
    26221 ttcaccgacc gaggtttcac ctttttcact aacgaacgct aaagcagcag gcgctacgcg
    26281 ctgataaagt ttgccgataa aggtttggta gtcgcgcaat atatccgtaa ccgtcgtctc
    26341 gatttttgtg cagatgcgat tcgccatgcc gcagatgatg aaaaactggc aggcattggc
    26401 tttcattggg gattttccga ccagagtcat ttttcaacgg tatttaagca acgctttggg
    26461 atgacgccag gcgagtatcg acgtaaattc cgctaactaa ttcgcgttgc atgtacagca
    26521 acgcgaatta gttaaaacag atatttgact atgcattttt cagggcgaaa ggcaatttga
    26581 caggaatttt tgaatattca cgttgtttcc ggaaaataga ttaatcgtct tctcctgtag
    26641 cgttatcccg atattgcggt tttgctatta aaattttcgc acccatttac cgtttatccc
    26701 ttgtacatat tccccgggtt tggcccgttc tacaagcttt tgcccggcga gtttcgcgat
    26761 atcatccacc gacacattat tctgctttgc cagttgttga tagctcgctt tacgttcggc
    26821 attgatgtcg gttactaatt tctcagtttc agcatctgtt ttcagcgcaa ccagataacc
    26881 ataaaatgtt tcgcctaccc gcccctgagt tctggcttca tccagagtca atgccattac
    26941 attgctgctt accaagccaa caagaaatgc acaaagaagt aatgttcttt tcatcatcac
    27001 ctcaaaaaag atcgctacgg gtctcaagca gctcttcgac atctttgtct gccttgatga
    27061 tgatctcatg ctcaatttta acgttcatat tgatagtgat cggttcctta ggtgcagcga
    27121 cttcaatgcg aggcgtacag ccaaccagca taaaagatga cgtcaacgca gccagtaaaa
    27181 ttttcattgt ttatcctcac actcttttcc ttgcggacag tcatttcccg gtagatgcgc
    27241 gttctgctcc agccatgcct ggagattatc gccaaagcgt aaactgcgcc acagcgtaaa
    27301 aatattctct tcatgatggt aattaagatt taccgtaccg cttttaccat caacgcgact
    27361 ggtacctgta atattggcct gcatggttaa taaaccgaga ttatctaaat taatttttgt
    27421 cgatgaacgg ctaatttcca tatagcgcaa ccagttaatt gctgaacccg cggtcatatt
    27481 gtctttcacc accgcatctg cggtgtcttt atccagccgc aaggtcattg gcccgctatt
    27541 tgccagccag ccgtctttca ctatccattt ttcgttgttt agccataacg gcaatgcacc
    27601 actaaatgcc ccggacatgg cgaattgttt cggattgacg gcgctaacca gttcgctgga
    27661 tgagagatta ttcagccgca acagcgccga gtcatgttgc ggcatgcgta attgtttcat
    27721 cagcacgtta ccgcctaaca catcgacgct aacatcagtc aacagcaatg gttcgtcttc
    27781 actccacgga taacgccctt gcaaatcagc cgtaatattt ttcgctgtca ccagattaat
    27841 cacttcggca attcgcaacg taacggggcc gcgagtcccc agatgccagg ctccatcggc
    27901 aaaacggaaa ggcaggacaa aatcgacacc gttaacctga ttatctggca tccaggcact
    27961 accgcctttc aacacaccgt gccctcccgc gcggaatcct tgttcaggcg cagctgaaaa
    28021 tgcaacctga gcatacagtt cgccatcgcg taagttcatc ttccagtcgg gtggcaccag
    28081 cggctggaat acagtcagtg attgtttagg ccaccaggcg ttaccgcgca gacgaatacc
    28141 gtcccagcga ccatttaccc gaaccgggcc aatctcacca gcatgcaaat cgcctttaaa
    28201 gaggaaatac gtcggatcgc gcccatcgac actaaatttt aaggttgatg gcggtaacac
    28261 actgccgcca gtgaacagcg tttgcccggc gtccagtgac agcgcgccgc taaaggaggg
    28321 atgctgtgcg tcacgtaccc agacgacggg cttgtcgaga attaatcgcg gcttttctac
    28381 cgtcatcgta ccgtattgta actggtcgaa accggtggag agatcggtca gcgtaatcgt
    28441 gctgtcatgc cactcaccct ttcctgcgac atcccaggtg gcattcatcg gtgtaaaact
    28501 gccttttccc cagtagcgcc actgccagcg gccagcgtca gggagaaaat cattcgccag
    28561 tccatccata tgcagcacga aatcgcccag ttcattttca tgcgcctgca aaatggcctg
    28621 caaatgtccg tcaacaccac gttgggtgac ttttacaccc gctaaaggcc agcggatttc
    28681 atcgatgtcc agcgaatcga tgactcttcc cttcgaacgt aataacgcgc ctggctcaaa
    28741 ggccagcgtt gggtcagtca gacttccgct tagctgcgca ggtaaacggg catataaaat
    28801 gaggtccgcc tgtttcgctt caccggtcag ctgcaaaggc agctggctgt tatccatact
    28861 taattttcct gggccaaaat taagcaccgc gttgccctta cccgcttgcc cctgggtcag
    28921 cacactcagt cgtccgctga ccagagcgtt ctcgagccct gcctgccagt tgtcgacttt
    28981 gacacccagt cgtccactca aaggaaaacc tgcatacggc cagctccagc gaccatcgct
    29041 tacggtcaat tgttggcgag taatttgcca cggcagatcg agcaacggat cgccgttatc
    29101 ccgtgccacc acaatcaatt gcccgctatt ttcctgccag tccagctcgg catccaccag
    29161 tgaaggctcc tgcggcaagt ttaacgtcgc agtagcatga ccacttacag gaagtccatc
    29221 cggcactagc ggcatagtaa attcccccac cagtttaacc ggcggctgat tttcaaacgc
    29281 gacgacatcc agttcgctga ctgtaagttg ttgccctttc agctggcctt gaaatttaac
    29341 tttttcgccc tgataacgca gttgctggat atcggaggtt aatgcgagag agagttttcc
    29401 ctgccattcc tgccagggag aaaaaatcag tttatcgata ttgatccagg tgttaggcaa
    29461 catggcctgc cactgcgcga gggtttttgg agcggcaggc gattgctccg tctgcggcaa
    29521 tttcgccaga caagcagaat caagttctac cgtgccgacg ttcaataacc agcggctggg
    29581 atgtgaaagg ctggcgttgg tgatatgcgc aagctgacaa tctcccacca gataacggag
    29641 atcgggaatg attaaacctt tacgcgtaat gcgtgggctt tcatctaatg caatacgtgt
    29701 cccgagcggt agccagatgc ccgccagcgt gggaacccac agcccgagcg tcatcagcag
    29761 tgtcaacggc acaagaataa tcagtaataa tagcgcgaga acggctttat atttacccag
    29821 catgggtagt taatatcctg atttagcgaa aaattaagca ttcaatacgg gtattgtggc
    29881 atgtttaacc gttcagttga aggttgcgcc tacactaagc atagttgttg atgaattttt
    29941 caatatcgcc atagctttca attatatttg aaattttgta aaatattttt agtagcttaa
    30001 atgtgattca acatcactgg agaaagtctt atgaaactcg ccgtttatag cacaaaacag
    30061 tacgacaaga agtacctgca acaggtgaac gagtcctttg gctttgagct ggaatttttt
    30121 gactttctgc tgacggaaaa aaccgctaaa accgccaatg gctgcgaagc ggtatgtatt
    30181 ttcgtaaacg atgacggcag ccgcccggtg ctggaagagc tgaaaaagca cggcgttaaa
    30241 tatatcgccc tgcgctgtgc cggtttcaat aacgtcgacc ttgacgcggc aaaagaactg
    30301 gggctgaaag tagtccgtgt tccagcctat gatccagaag ccgttgctga acacgccatc
    30361 ggtatgatga tgacgctgaa ccgccgtatt caccgcgcgt atcagcgtac ccgtgacgcc
    30421 aacttctctc tggaaggtct gaccggcttt actatgtatg gcaaaacggc aggcgttatc
    30481 ggtaccggca aaatcggtgt ggcgatgctg cgcattctga aaggttttgg tatgcgtctg
    30541 ctggcgttcg atccgtatcc aagtgcggcg gcgctggaac tcggtgtgga gtatgtcgat
    30601 ctgccaaccc tgttctctga atcagacgtt atctctctgc actgcccgct gacaccggaa
    30661 aactatcatc tgttgaacga agctgccttc gaacagatga aaaatggcgt gatgatcgtc
    30721 aataccagtc gcggtgcatt gattgattct caggcagcaa ttgaagcgct gaaaaatcag
    30781 aaaattggtt cgttgggtat ggacgtgtat gagaacgaac gcgatctgtt ctttgaagat
    30841 aaatccaacg acgtaattca ggatgacgta ttccgtcgcc tgtctgcctg ccacaacgtg
    30901 ctgtttaccg ggcatcaggc attcctgaca gcagaagcgc tgaccagtat ttctcagact
    30961 acgctgcaaa acttaagcaa tctggaaaaa ggcgaaacct gcccgaacga actggtttaa
    31021 tcttgccgct cccctgcatt ccaggggagc tgattcagat aatccccaat gacctttcat
    31081 cctctattct taaaatagtc ctgagtcaga aactgtaatt gagaaccaca atgaagaaag
    31141 tagccgcgct cgttgcgcta agcctgctga tggcgggatg tgtaagtaat gacaaaattg
    31201 ctgtaacccc agaacagcta cagcatcatc gctttgtgct ggaaagcgta aacggtaagc
    31261 ccgtgaccag cgataaaaat ccgccagaaa tcagctttgg tgaaaagatg atgatttccg
    31321 gcagcatgtg taaccgcttt agcggtgaag gcaaactgtc taatggtgaa ctgaccgcca
    31381 aagggctggc aatgacccgt atgatgtgcg ctaacccgca gcttaatgaa ctcgataaca
    31441 ccattagcga aatgctgaaa gaaggtgcac aagtggatct gaccgcgaac cagttaacgc
    31501 tggcgaccgc gaaacagaca ttaacttata agctggcgga tttaatgaat taatagctgc
    31561 cacagctccc ggcggcaagt gactgttcgc tacagcgttt gccgttgggt aatgcacaca
    31621 tcccaatcgt cgtaccatcc agttgacggg caacagaaag cgaaccgccg atcattgcac
    31681 aatttgcttc tccactactg gacatcgacg cttttaaacc tggcgctacg tgcgccgcag
    31741 tcgcctgctg aacaggttca ctactacacg ccgacaacaa taaagcggca caccctaccc
    31801 aaaacgctgc tcgcatcttt tcttcctctg atcttcaagc caaacgacac cgccataaat
    31861 aataggcagc acagagggcg gcgtcgagag ctgtcctgag cgttgccccg catttttact
    31921 tttttatggc tatttttttg ccctctgttt gatcaaaaca ttcattacgc tgatgtgggg
    31981 gacacaaaag cgaaaatgca gaagaaagcc atttgctaaa attgaaggat tactactggg
    32041 cgcgcagcaa tttcgtgcgc ccctcatttg cgcaatgtaa gggtgtcata tgattactat
    32101 tgacggtaat ggcgcggttg cttcggtggc gtttcgcacc agtgaagtta tcgccatcta
    32161 ccctattacc cccagttcca cgatggcaga acaggctgat gcctgggccg gaaacggctt
    32221 gaagaacgtt tggggagaca caccacgcgt ggttgaaatg cagtcggaag cgggtgctat
    32281 cgctaccgtg catggcgctt tgcagacggg tgccctctca acatcgttta cgtcatcgca
    32341 gggtttgctg ctgatgatcc cgacgctgta caaactggca ggcgaactga cgccatttgt
    32401 cctgcatgta gcggcacgta ccgttgccac acatgcactc tctatttttg gcgatcattc
    32461 cgacgttatg gcggtgcgcc agacgggttg cgcgatgttg tgtgcggcta acgtccagga
    32521 agcgcaagac tttgctctca tttcgcacat cgcaacgctg aagagccgcg tgccatttat
    32581 tcatttcttt gatggtttcc gcacgtccca cgaaatcaat aaaattgtcc cgctggccga
    32641 tgacactatt cttgatctca tgccgcaggc cgaaattgat gctcatcgcg cccgggcgct
    32701 caacccggaa catccggtga tccgcggtac gtccgccaat cctgacactt atttccagtc
    32761 tcgcgaagcc accaacccat ggtacaacgc agtctatgac catgttgaac aggcgatgaa
    32821 tgatttcgct gccgcgacag gtcgccagta tcagccgttt gagtattacg ggcatccaca
    32881 agcggaacgg gtgattatcc tgatgggctc tgccattggc acctgtgaag aagtggttga
    32941 tgaattgctg acccgtggcg aaaaagtcgg cgtgctgaaa gttcgcctgt accgcccctt
    33001 ctccgccaaa cacttactgc aagcgttgcc ggactctgtt cggacagtgg cggtgctgga
    33061 tcgcaccaaa gagccaggtg cgcaagcgga acccctgtac ctggatgtga tgaccgcact
    33121 ggcagaagcc tttaataatg gcgagcgtga aaccctgccc cgtgtcattg gtgggcgcta
    33181 tggtctttca tccaaagaat ttggcccgga ctgtgtactg gcggtatttg ccgagctcaa
    33241 cgcggctaaa ccgaaagcgc gctttacggt aggtatttac gatgatgtga ccaatctgtc
    33301 actgccgttg ccggaaaaca ccctgccaaa ctcggcgaaa cttgaagcct tgttttatgg
    33361 ccttggtagt gatggcagcg tttccgcgac caaaaacaat atcaagatta tcggtaattc
    33421 cacgccgtgg tacgcacagg gctattttgt ttacgactcc aaaaaggcgg gcggcctgac
    33481 ggtttctcac cttcgcgtga gcgagcagcc gattcgttcc gcttatctga tttcccaggc
    33541 tgattttgtt ggctgccacc agttgcagtt tatcgataaa tatcagatgg ctgaacgttt
    33601 aaaacctggc ggcattttcc tgctcaacac gccgtacagc gtagatgaag tatggtcgcg
    33661 cttgccgcag gaagttcagg ccgtgttaaa ccagaaaaaa gcgcgcttct atgtgattaa
    33721 cgcggcgaaa atcgcccgcg aatgtggtct ggcggcccgt attaataccg tcatgcagat
    33781 ggcttttttc catctgacgc aaattctgcc tggcgatagc gccctcgcag aattgcaggg
    33841 tgcgattgcc aaaagttaca gtagcaaagg tcaggatctg gtggagcgca actggcaggc
    33901 tctggcgctg gcgcgtgaat ccgtagaaga agttccgttg cagccggtta atccgcacag
    33961 cgccaatcga ccgcctgtgg tttccgatgc cgctcctgat ttcgtgaaaa ccgttaccgc
    34021 ggcgatgctc gccgggcttg gtgatgccct ccccgtttcg gcgctgccgc cagacggcac
    34081 ctggccgatg ggcaccacgc gctgggaaaa acgcaatatc gccgaagaga tccccatctg
    34141 gaaagaggaa ctctgtaccc aatgtaacca ctgcgtcgct gcttgcccac attcggctat
    34201 tcgcgcaaaa gtggtaccgc ctgaagcgat ggaaaacgcc cctgccagcc tgcattcgct
    34261 ggatgtgaaa tcgcgtgata tgcgcgggca gaaatatgtc ttgcaggtgg caccggaaga
    34321 ttgcaccggc tgtaacctgt gcgtcgaagt ttgcccggcg aaagaccgtc agaatccaga
    34381 gattaaagcc atcaatatga tgtcgcgcct ggaacatgtc gaagaagaga aaatcaatta
    34441 cgatttcttc ctcaacctcc cagaaatcga ccgtagcaaa ctggaacgta ttgatattcg
    34501 tacatcacag ctgattacac cgctgtttga atattcaggt gcttgctccg gttgtggcga
    34561 gacgccgtat attaaattgc tgactcagct ctatggcgac cgaatgttga tcgcgaacgc
    34621 caccggctgt tcttcaattt atggcggtaa cctgccctct acaccgtata ctaccgatgc
    34681 caacggtcgt ggaccggcat gggcgaactc gctgtttgaa gataacgccg aatttggcct
    34741 tggtttccgc ctgacggtcg atcaacaccg tgtccgcgtg ttgcgtctgc tggatcaatt
    34801 tgccgataaa atcccggcgg aattactaac tgcgttgaaa tcagacgcca cgccagaggt
    34861 tcgtcgtgaa caggttgcag ctttacgcca gcaactcaaa gatgttgccg aagcacatga
    34921 actgctacgt gacgcagatg cactggtaga aaaatcaatc tggctgattg gtggtgatgg
    34981 ctgggcttac gatatcggct ttggcggtct ggatcatgta ttgagtttga cggaaaacgt
    35041 caacattctg gtgctggata cgcaatgcta ttccaacacc ggtggtcagg cgtcgaaagc
    35101 gacaccgctg ggtgcagtaa ctaagttcgg cgagcacggc aaacgtaaag cgcgtaaaga
    35161 tcttggcgtc agtatgatga tgtacggtca tgtttatgtg gcgcagattt ctctcggcgc
    35221 gcagctcaac cagacggtga aagcgattca ggaagcggaa gcgtatccgg ggccatcgct
    35281 gatcatcgct tatagcccct gtgaagagca tggttacgat ctggcactca gtcacgacca
    35341 gatgcgccaa ctcactgcca ccggcttctg gccgctatat cgcttcgatc cacgtcgagc
    35401 cgatgaaggc aaactgccgc tggcattgga ttcacgcccg ccgtcagaag cactggaaga
    35461 aacgttactt cacgagcagc gtttccgtcg tctgaattcg cagcagccag aagtggcaga
    35521 acagttatgg aaagatgctg cagctgattt gcaaaaacgc tatgacttcc tggcacaaat
    35581 ggccggaaaa gcggaaaaaa gcaacaccga ttaaatgcac tggataagga ttatccaatt
    35641 ctaaaaaaaa gcccggacga ctgttcgggc ttgtcttttt atatgttgga aaatcagtag
    35701 caatgcaatg gcccaacaga aaataaaatc tgtgacaata aaggcat
//
LOCUS       DAZGQL010000053        35362 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.053, whole genome shotgun sequence.
ACCESSION   DAZGQL010000053 DAZGQL010000000
VERSION     DAZGQL010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35362
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.053"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..218)
                     /locus_tag="ACHKIR_002800"
     CDS             complement(<1..218)
                     /locus_tag="ACHKIR_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9990510.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDLALPGPLPF"
     gene            complement(421..822)
                     /gene="nikR"
                     /locus_tag="ACHKIR_002801"
     CDS             complement(421..822)
                     /gene="nikR"
                     /locus_tag="ACHKIR_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HFO9990511.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(827..1633)
                     /gene="nikE"
                     /locus_tag="ACHKIR_002802"
     CDS             complement(827..1633)
                     /gene="nikE"
                     /locus_tag="ACHKIR_002802"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HFO9990512.1"
                     /translation="MTLLNVCDLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWRGEPLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKAEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQVVGDKLTFSSDAGRVLQNAVLPAFPVRRRTTEKV"
     gene            complement(1630..2394)
                     /gene="nikD"
                     /locus_tag="ACHKIR_002803"
     CDS             complement(1630..2394)
                     /gene="nikD"
                     /locus_tag="ACHKIR_002803"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HFO9990513.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLKRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENTARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSHGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            complement(2394..3227)
                     /gene="nikC"
                     /locus_tag="ACHKIR_002804"
     CDS             complement(2394..3227)
                     /gene="nikC"
                     /locus_tag="ACHKIR_002804"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015099 - nickel cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HFO9990514.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLIIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFIDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(3224..4168)
                     /gene="nikB"
                     /locus_tag="ACHKIR_002805"
     CDS             complement(3224..4168)
                     /gene="nikB"
                     /locus_tag="ACHKIR_002805"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HFO9990515.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(4168..5742)
                     /gene="nikA"
                     /locus_tag="ACHKIR_002806"
     CDS             complement(4168..5742)
                     /gene="nikA"
                     /locus_tag="ACHKIR_002806"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO9990516.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKNELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWVLQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKALLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(5853..6440)
                     /gene="acpT"
                     /locus_tag="ACHKIR_002807"
     CDS             complement(5853..6440)
                     /gene="acpT"
                     /locus_tag="ACHKIR_002807"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HFO9990517.1"
                     /translation="MYRIVLGKVSTLSAAPLPPALREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLDNLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(6495..7544)
                     /locus_tag="ACHKIR_002808"
     CDS             complement(6495..7544)
                     /locus_tag="ACHKIR_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFO9990518.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGIYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            7676..8893
                     /locus_tag="ACHKIR_002809"
     CDS             7676..8893
                     /locus_tag="ACHKIR_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9990519.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(8897..9454)
                     /gene="dcrB"
                     /locus_tag="ACHKIR_002810"
     CDS             complement(8897..9454)
                     /gene="dcrB"
                     /locus_tag="ACHKIR_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HFO9990520.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADNQQKAQTTAENIINTLVIQ"
     gene            complement(9527..10192)
                     /locus_tag="ACHKIR_002811"
     CDS             complement(9527..10192)
                     /locus_tag="ACHKIR_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HFO9990521.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRHWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            10414..10659
                     /gene="tusA"
                     /locus_tag="ACHKIR_002812"
     CDS             10414..10659
                     /gene="tusA"
                     /locus_tag="ACHKIR_002812"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HFO9990522.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDELPYRYLIRKGG"
     gene            complement(10761..12959)
                     /gene="zntA"
                     /locus_tag="ACHKIR_002813"
     CDS             complement(10761..12959)
                     /gene="zntA"
                     /locus_tag="ACHKIR_002813"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HFO9990523.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSTPTLSEN
                     VSSTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQSADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVMLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPAAQSQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGVIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTELNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(13033..13659)
                     /locus_tag="ACHKIR_002814"
     CDS             complement(13033..13659)
                     /locus_tag="ACHKIR_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HFO9990524.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            13800..14159
                     /locus_tag="ACHKIR_002815"
     CDS             13800..14159
                     /locus_tag="ACHKIR_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HFO9990525.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDI
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(14162..14431)
                     /locus_tag="ACHKIR_002816"
     CDS             complement(14162..14431)
                     /locus_tag="ACHKIR_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HFO9990526.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(14421..15017)
                     /gene="rsmD"
                     /locus_tag="ACHKIR_002817"
     CDS             complement(14421..15017)
                     /gene="rsmD"
                     /locus_tag="ACHKIR_002817"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HFO9990527.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            15167..16663
                     /gene="ftsY"
                     /locus_tag="ACHKIR_002818"
     CDS             15167..16663
                     /gene="ftsY"
                     /locus_tag="ACHKIR_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFO9990528.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     AKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPELVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            16666..17334
                     /gene="ftsE"
                     /locus_tag="ACHKIR_002819"
     CDS             16666..17334
                     /gene="ftsE"
                     /locus_tag="ACHKIR_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFO9990529.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            17327..18385
                     /gene="ftsX"
                     /locus_tag="ACHKIR_002820"
     CDS             17327..18385
                     /gene="ftsX"
                     /locus_tag="ACHKIR_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFO9990530.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            18630..19484
                     /gene="rpoH"
                     /locus_tag="ACHKIR_002821"
     CDS             18630..19484
                     /gene="rpoH"
                     /locus_tag="ACHKIR_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HFO9990531.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            19756..20859
                     /gene="livJ"
                     /locus_tag="ACHKIR_002822"
     CDS             19756..20859
                     /gene="livJ"
                     /locus_tag="ACHKIR_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HFO9990532.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQVGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(21047..21430)
                     /gene="panM"
                     /locus_tag="ACHKIR_002823"
     CDS             complement(21047..21430)
                     /gene="panM"
                     /locus_tag="ACHKIR_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0031638 - zymogen activation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HFO9990533.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            21854..22963
                     /gene="livK"
                     /locus_tag="ACHKIR_002824"
     CDS             21854..22963
                     /gene="livK"
                     /locus_tag="ACHKIR_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HFO9990534.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQYIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANKGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            23011..23937
                     /gene="livH"
                     /locus_tag="ACHKIR_002825"
     CDS             23011..23937
                     /gene="livH"
                     /locus_tag="ACHKIR_002825"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HFO9990535.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            23934..25211
                     /gene="livM"
                     /locus_tag="ACHKIR_002826"
     CDS             23934..25211
                     /gene="livM"
                     /locus_tag="ACHKIR_002826"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HFO9990536.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASEIR
                     WQWVFIGTAVVFFFQLVRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGSFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            25208..25975
                     /gene="livG"
                     /locus_tag="ACHKIR_002827"
     CDS             25208..25975
                     /gene="livG"
                     /locus_tag="ACHKIR_002827"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HFO9990537.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            25977..26690
                     /gene="livF"
                     /locus_tag="ACHKIR_002828"
     CDS             25977..26690
                     /gene="livF"
                     /locus_tag="ACHKIR_002828"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HFO9990538.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            26778..26999
                     /locus_tag="ACHKIR_002829"
     CDS             26778..26999
                     /locus_tag="ACHKIR_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFO9990539.1"
                     /translation="MHTMNYSEARQNLALVLETAATGTPVTITRRGHKAAVIISAEEF
                     ERYQKAKLDAEFDAIMAIHGSEIKELADK"
     gene            26996..27373
                     /locus_tag="ACHKIR_002830"
     CDS             26996..27373
                     /locus_tag="ACHKIR_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFO9990540.1"
                     /translation="MTIQFISAEEIIYFHDKLLSVTPGVTGMSDPGRAEALLYRVLNK
                     YEYEGVSDLWLLAAMHLLAISRGHIFNDGNKRTALFITLLFLKRNGIILAANPEFVEL
                     TVDVAAGKVSLEDIVTRLRQRGT"
     gene            27582..28898
                     /gene="ugpB"
                     /locus_tag="ACHKIR_002831"
     CDS             27582..28898
                     /gene="ugpB"
                     /locus_tag="ACHKIR_002831"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HFO9990541.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYAAKLKASGMKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEQSTKS"
     gene            29074..29961
                     /gene="ugpA"
                     /locus_tag="ACHKIR_002832"
     CDS             29074..29961
                     /gene="ugpA"
                     /locus_tag="ACHKIR_002832"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HFO9990542.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            29958..30803
                     /gene="ugpE"
                     /locus_tag="ACHKIR_002833"
     CDS             29958..30803
                     /gene="ugpE"
                     /locus_tag="ACHKIR_002833"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HFO9990543.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            30805..31875
                     /gene="ugpC"
                     /locus_tag="ACHKIR_002834"
     CDS             30805..31875
                     /gene="ugpC"
                     /locus_tag="ACHKIR_002834"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HFO9990544.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTTGDIWIDRKRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVREPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLKGRVNNEGTHFELDGGIALPLNGGYRQYAGRKM
                     TLGIRPEHITLRSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVLRLAHQERPTAGS
                     TLWLHLPENQLHLFDGETGQRV"
     gene            31872..32615
                     /gene="ugpQ"
                     /locus_tag="ACHKIR_002835"
     CDS             31872..32615
                     /gene="ugpQ"
                     /locus_tag="ACHKIR_002835"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFO9990545.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGARYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKTVALAARELWTGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(32602..33042)
                     /locus_tag="ACHKIR_002836"
     CDS             complement(32602..33042)
                     /locus_tag="ACHKIR_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HFO9990546.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            33162..34907
                     /gene="ggt"
                     /locus_tag="ACHKIR_002837"
     CDS             33162..34907
                     /gene="ggt"
                     /locus_tag="ACHKIR_002837"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HFO9990547.1"
                     /translation="MIKPTFLRRVAIAALFTGSCFSTVAAPPAPSPVSYGVEEDVFHP
                     VRAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLI
                     RSKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALD
                     KYGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGAPLK
                     KGDKLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVER
                     TPISGEYRGYQVYSMPPPSSGGIHIVQILNILENFDMQKYGFGSADAMQIMAEAEKYA
                     YADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAKPSSEIRPGKLAPYESNQT
                     THYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGG
                     DANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAE
                     ATNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELY
                     GASDPRSVDDLTAGY"
     gene            complement(34945..35229)
                     /locus_tag="ACHKIR_002838"
     CDS             complement(34945..35229)
                     /locus_tag="ACHKIR_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhB family protein"
                     /protein_id="HFO9990548.1"
                     /translation="MITYHDAFAKANHYLDDADLPVVITLHGRFIQGWYFCFESQEFL
                     ESGDEAARLAGNAPFIIDKDSGEIHSLGTAKPLEEYLQDYEIKKATFGLP"
BASE COUNT         8245 a         9113 c         9891 g         8113 t
ORIGIN      
        1 atgaacggca gcgggccggg cagggcaagg tcggtttcgc cgggaaggac cttcgcaccg
       61 agcagcggat tgaccggatg accggacgtc accccgccgg ggcacaccga acaggccaca
      121 ccggtggggg caccaatacg taccccggca gaaccctgaa caatgctacc gccatactgc
      181 gtcatgtcgc cctgacgcgc cgccggtttt ccgctcatcg taaaatcctt ttctattatt
      241 ttaatgacag tatcttcata cgaaaaagcg aagaaaaact tgtggtcaca atgaattgaa
      301 gatttttagg aatatattta gaacgttaca aaagagaagg ttcggatagg gtatttagag
      361 aggaatcagg tgtgctagtg gccctttgtt gatcgttcac caaaggaccc aataataggc
      421 ttaatcttcc ttcggcaagc actgcaaatg cccgtgccgc acgccgcgct gggcgataac
      481 gtcatcggca aaatgctgca cgtcgcccat atcgcctttc agcacggcga tttccagaca
      541 gtcgtcgtga ttgatgtgga catgtagcgt cgcgacggag agatcgtggt gatggtgctg
      601 ggtggagaca atacggctgg ctaagtcgcg tttttcgtgt tcatacacat aggagagcac
      661 cgcgaaacct tgcgtgccgt gctgctgggt agcttcctgc gccagggcgc tacgcagaat
      721 atcgcggata gcttcggaac ggttgttata gccacgacgc tggctcaggc tgtccagcgt
      781 ctccagtaaa tcgtcatcaa gcgtgatggt aactcgttgc atagacttaa accttttctg
      841 tggtgcgacg gcgcacgggg aatgcgggta ataccgcgtt ttgtagcaca cgtccggcgt
      901 cagaggaaaa ggttaattta tctcccacca cctgggtttc gacgatttgt ccgttgtcca
      961 tcaccatcac ccgctgacag aagcgttcca ccaggcgtaa gtcgtgggtg atgaacaggc
     1021 aggcggtgcc aaactgttgt tgcagctttt tcagcaggcg aatgacgccc gcctgtaaca
     1081 cgagatcaag gttagaaacg gcttcatcca gaatcagtag tttcggttcg accgccagcg
     1141 cgcgagccag gcagacgcgc tggagctgac cgccgcttaa ctgcggtggg cgtttgtcta
     1201 gaacgctgtc atcgagatcg accgccttca gcatttcgct ggcacgcgcc agttgttcgg
     1261 cttttttcag cgatagcagg tggcgcatcg gttcacgcag gatctcgcgc acggtttttc
     1321 gcggattcac ggcgctgatg gagtcctgaa ataccatctg aatatcgcgg cggaacgctt
     1381 tacgctgggc gcgattgagc tttgccaacg gttcgccgcg ccagctaata ttcccttgtg
     1441 agggagactc taagcctacc agcagccgcg cgagagtgct ttttccgcag ccgctgcgcc
     1501 ccagcagagc gacggtttcg ccgcttttca gggtcaggga aacgttattc agtaccgcct
     1561 gatgctgatg ttttccgcta aatccaccgt gcgcatagtg atgtgaaagg tcgcagacgt
     1621 taagtaaagt catgatgcca gctccatacc gtagagggcg agatgagcgg aaaccaggct
     1681 gcgcgtcacc gtatgtttgg gggcgttaaa cagcgtttct acatcgccct gttcgacaat
     1741 tttaccgtgt gacatcaccg ccacgtcatc cgccagacgc gccacaacgc ccatatcatg
     1801 ggtgaccagc agcattcccg gtgcttgttt ttgcataatg ctttccagca gatcgaggat
     1861 gcgcgcctgt gctaccacgt cgaggtcggt ggtcggttca tcggcgatga taaacggcga
     1921 ttcacacaac accgccatcg caatcatcat gcgctgcaac atgccgccgc tcatctcgaa
     1981 cgggtacagc ttcaacacgc gcgcggtgtt ttccaacccc accgcttcta tggcagcggt
     2041 aagcgtagcg tcatcggcgg gtttccccag cgccaggcag gtttcacgcg cgtgggtgtg
     2101 catggtgtgt agcggattaa aggcgctgcg cgggttctgc atgatggtgg caattttgat
     2161 gccgcgcagg gcacagggag aaaccggttt gccatcggct aaaatttccc ccgccgtctg
     2221 gcgaacgcct gcgggcagaa tgcccagcgt cgcggcgcag gtcagtgatt tcccgctgcc
     2281 gctaccgccg actaacgcca gcacacgccc gcgtttaagg gttaacgata cgccgtgcac
     2341 cagcggttgt gcggcctgta gcgcgatatt acgtagttca atctgttgcg gcattagtgt
     2401 gcgtgctccg tcaccagatg aggatccaga tgatcgcgca gtgcgtcacc caccaggtta
     2461 aaggccatca cgctgataaa cagcgccagc cccggccaga acatttgcag cggctgggtc
     2521 cagatatact gacgcgcgtc gttaatcatc acgccccatt cggcggtcgg cgcggtcacg
     2581 ccgagaccga ggaaggacat ccccgcgacg tgtagcatca tatgaccgat atccagcgtc
     2641 gccagcacca gcagcgaggg gatcaccgca cctgccagat gatcaataaa cacccgcaca
     2701 tggcccgcgc cagaaagccg tgatgccagc acaaactcgc gctggcgcag tgaaatcacc
     2761 aggctacgca ccatgcgtgc ataccacgcc cagtgcgaca gggcgatggc tataatcaca
     2821 ttggtcaacc cggtgccgag cacgccgacc ataaagaacg acagaatcga ggttgggaag
     2881 gtcataaaca tatcagcgac gcgcatggtg gcctgatcaa cgcgcccgcc aatcagcccg
     2941 gcgctgccgc caataatcag ccctaatgtc agcaccaaca gcaggcaggc cattaccgag
     3001 ccgagcgaca cgcgggtcgc cgccatcagc cgcgagaaaa tatcgcgccc taaatgatcg
     3061 gtacccagcc agtgctgcgc atccggtgaa agcaggcgcg acggcagatc aatcgcctgg
     3121 gggtcatacg gcagccacca ctggctggtg agcgcaatca gcgccagcag ggcgataatg
     3181 atcagcgcca ggcgtaccga ccagcgggaa gagaggaaaa agttcacgcg tgcgctcctt
     3241 catgacgacg aatgcgcggg tccagcgcgg cgttgagtaa atcaacaatc aaattacaga
     3301 ccacaaaaac caccaccatc atcagcgtaa agcactggat caccggatag tcacggttaa
     3361 aaatcgccga caccgcatag cgcccgacgc ccggccaggc aaagatgttt tcgataatca
     3421 tcgttccgcc aatcagttcg ccaatgtgca tccccacggc ggtgatcatc ggcagcgagg
     3481 cattgcgcag aatatggcgg cgttcggtct gtttgtcgtt caggccgcgt agccgagccc
     3541 aggtgacgtg acgctgaccg gcgacgtcca gcatactggc gcgcagcagc cgcgcgttaa
     3601 tcgccagcga cataaaggca atggaaaccg caggcaaaat gatgtgctgc cagccgccgt
     3661 agcccatcgc gggtagccat tgcagatata ccgaaaacgc catcaccagc aaaaacgcca
     3721 gccagaagtt gggcatcgac acgccgagaa acgcgatgaa acgtacggcg aaatccggca
     3781 gacgatcgcg atggcgcgcc gcccagatac cgagcggtac ggaagtgagc agaattaaca
     3841 ccagcgcggc acctgcaagt tccagcgtgg cgggcaggaa gttcagcata tcgtccagca
     3901 ccgggcgttg gctggcgaat gagataccaa agtcaagatg cagcgccttc cacaaccagg
     3961 taccgtactg gacgtacagc ggctgatcca gccccagcat ggtgcgggta gaggccagca
     4021 tctccggcgt cggcggcagg ttagataaac gcagataatc gagcgccggg tcgccggtac
     4081 cgaggcgcag catcagaaaa atgatcaccg aggcggcgag caccatcggg atcagcagca
     4141 gaaagcggcg taatacgtaa cgcaacatta aggtttcacc ggtttaatct gttcgaacgg
     4201 aatttcggtg gcgatcggcg cgtaggggat gttacccagc tccggttttg ataccaccat
     4261 cattgagatg taactgatag gcagataaac cgcctcgtca tgcagacggg tcagaatgtc
     4321 gcgatacagc gcctgacgtt gcgtttcgtc atgggtcgcc agcacttcgc cgatctcttt
     4381 atcaatcagc ggcttgtcgg ctaaaccttg ctgtgcctgg aagtcagcgt gcgacggtac
     4441 gcgcattgaa ctcaggaagg cgtgtggatc gtatggcgcg ccccaggtgc ggtggaaaat
     4501 catgccaaaa cgaccgtcgc gctgacgagc atagatactg ctctcttctt cgccaatcag
     4561 cgagacatct gcgccaatct ggcgcatatc agcctgaatg atttccgcca tcgatttgct
     4621 taacgcatcg gtgccgatga acgacagttc aatgcgcagc ggctgaccat ttttctcgcg
     4681 gatgtctttg cctgcaggca gcgtccaacc ggctttttcc agcaacgctt tcgctttctg
     4741 cggatcgtac tggcgcggtt tcaggccgag gttggcgtag ggcacagaag gggcaaacag
     4801 ggtgtcggcg acctgctggg taccatacaa cgcgttatca atcagcgatt ttttgttcac
     4861 cgcgtaatta agagcttcac gtactgccag ctcgttggtg ggggctttgg cggtattgag
     4921 cgccagcatc acggtttcga tcggctgtga cagttgagtg tggtaagccg ggttctggct
     4981 aaagcgggcg aaggtatcga gcggtaataa cccttcgttt ccgtacagca ggtcgatatc
     5041 gccagtttca aacgccaccg cacgggtagt cgggtccggg atcacgttaa aggtgatctt
     5101 tttaatcgct ggcttttcgc cccagtagtt ttcgttacgg acgaagacat cgtactgatt
     5161 cagtttcgat tcctgtaaaa cccacggccc ggtgccaatc ggcgctttaa ttccgttcat
     5221 ggtttcgtgg tttttaaact gcgagggtgc gataaaacgg aaaggacggg gcagggccag
     5281 ttcttgcagg aaaggatagt aggcgctttt cagggtgatt tgcagctcgt ttttactgag
     5341 tgctttgaca tcaacaatct ggtttgccag ctccagccag gcgtgacgtt gacggttatc
     5401 gagcactgcg cggaagtttt ctgccgccgc ctctgcatca aacggttcgc cgttggagaa
     5461 tttcacgtca tcacgcaggg tgaaggtcca ggttttacca tcttctgaat gagtccagct
     5521 ttttgccagc cacgggatca ccgaaccgtc tgcctgatat ttcaccaatg gttcataaac
     5581 catgctctgg gcgaacatct ggttaggcgt gtaaaggtgc gggtttagtg gcccgacatt
     5641 caccggccag gcggtggtga tttcatctgg tgcagcggca tggacgataa aagacgcaca
     5701 agccagcagc gcaaatagag tgcggcggag tgtggagagc atggtaaccc caatggatta
     5761 aaatagatgg cggaaataag tatgacgatt ttaagtattc gtcatactga ttacctgttc
     5821 tggatcaata gatgggcagt cgggtgggcg gatcagttaa ctgaatcgat ccattgcaca
     5881 ctgtcggcgg tgagcgtaaa gggggtaggg gtgcagatcg ccaggcttaa attgtctagc
     5941 tgacaatggc tgactgacag tgatgagtga taggtgctgt ctacgctgac gatttgccag
     6001 gcgctgccgc cacgctgttt aacgatggct tctttgcgcg tccagatgcg ccagaacatt
     6061 tccagttgct gatcaggatg cacggcgtcc atctcggcat gttccccaag gctgaatacg
     6121 gcgttcgcca gccagcgcca gttggcgcgc gggcgaatca cttcgatatc gcagccaact
     6181 tcgccttcat cgctcaacag cagggcgata tcgtcaccgc tatggcttaa gttgaaccat
     6241 agcggcgttt ccggcgcgaa tgccggtttg ccttgttcgc catagatgat ctccggtagc
     6301 ggggaaagcg tgtgcgaaag caatgcacgc cccgccagcc agcgttcgcg tcgtggacct
     6361 tgcggtgctt gctcgcgtaa agccggtggc agtggagctg cgcttaaggt cgaaactttc
     6421 cccagaacta tccgatacat atcagggcca acgtttaatg gaaaatgaaa gtgcgtatcg
     6481 taacacttgt cgcctcatcc cggtaaccga cttttcggtc tgcctggccc cagtaaaatc
     6541 gccagtttgc taccgccttt ggtggtttcc atccagattt tacacacgct ggttaatggc
     6601 accgaaagta gcatccctac cgggccgagc agccatcccc aaattaacaa tgaaagaaat
     6661 accaccatgg tggacatccc cagacgatgg cccatcatcc gtggttctaa aatattgccg
     6721 atgaccatat ggaccactaa aaacaatgcg ccgaccagaa tacattcgta aataccatta
     6781 aacagcagca cctgaatcat tggcggtacg gcggaaatta ccgcaccgat attgggcacg
     6841 tagttgagca aaaacgccag taccgcccac atcagcgcaa actgtacgcc cataagttcc
     6901 agccccagcc agacgataac gcctgtccat aaacttagca gtgttttcaa tgccagataa
     6961 tgcgaaacgc ctttaagtgc gcggtgtagt ccggcgatgt gaatttgtgg attattcaac
     7021 gcgaaacgca ttttgtaagg gacgtggcgc acttcaaaca gcataaaaac tacggtcatc
     7081 accagcaaaa gcacgctcgc cattgccccg gaaagcccgg tcattagcgc tgtggtgaag
     7141 gtaaccactt tttccgagtc catccgctgc agcattcgct ccggtgacat atgcaaatta
     7201 agaaaaggca acatctcctg caatttaaaa agtttgcgcg tcagctcctt attaaacttc
     7261 ggcagcatag agataaattc gttaaacgat gccgccagta cgccgaccag cgcagttagt
     7321 gcgatcagca tcaccaccac cacaatcgta atggcaacgg ggcgttgtac tccccgacga
     7381 ataaaccagg tgacgagcgg gttgaggacg atggcaaaaa acagcgccag taacagctgc
     7441 acaatgatat ctgccgctgc gtgaatgccc gcgaggatca ctaccagcga ggccagcttg
     7501 agcagaatgt gcatgcccgt tttatcgggt tgaggggttt ccattggggc ttccttgtga
     7561 ctttttgtat taagtgtagc gggagtcacg ccagcaatat tctgattctt actgctgatt
     7621 ttcacgtcag cacgtggtaa aaatgaaaca ctgttgtaaa aatgtggtga tcctcatgcc
     7681 cgaacccgta gccgaacccg cgctaaacgg attgcgcctg aatttgcgca ttgtctccat
     7741 tgtcatgttt aacttcgcca gctacctcac catcgggttg ccgctcgctg tattaccggg
     7801 ctatgtccat gatgtgatgg gctttagcgc tttttgggca ggattggtta tcagcctgca
     7861 atatttcgcc accttgctga gccgccctca tgccggacgt tacgccgatt tgctgggacc
     7921 caaaaagatt gtcgtcttcg gtttatgcgg ctgctttttg agcggtctgg gatatctgac
     7981 ggcaggatta accgccagtc tgcccgtcat cagcctgtta ttactgtgcc tggggcgcgt
     8041 catccttggg attgggcaaa gttttgccgg aacgggatcg accctgtggg gtgttggcgt
     8101 ggttggctcg ctgcatatcg ggcgggtgat ttcgtggaac ggcattgtca cttacggggc
     8161 gatggcgatg ggtgcgccgt taggtgtcgt gttttatcac tggggcggct tgcaggcgtt
     8221 agcgttaatc attatgggcg tggcgctggt ggccattttg ttggcgatcc cgcgtccgac
     8281 ggtaaaagcc agtaaaggca aaccgctgcc gtttcgcgcg gtgcttgggc gcgtctggct
     8341 gtacggtatg gcgctggcac tggcttccgc cggatttggc gttatcgcca cctttatcac
     8401 gctgttttat gacgctaaag gttgggacgg tgcggctttc gcgctgacgc tgtttagctg
     8461 tgcgtttgtc ggtacgcgtt tgttattccc taacggcatt aaccgtatcg gcggcttaaa
     8521 cgtagcgatg atttgcttta gcgttgagat aatcggcctg ctactggttg gcgtggcgac
     8581 tatgccgtgg atggcgaaaa tcggcgtctt actggcgggg gcagggtttt cgctggtgtt
     8641 cccggcattg ggcgtagtgg cggtaaaagc ggttccacag caaaatcagg gggcggcgct
     8701 ggcaacttac accgtattta tggatttatc gcttggcgtg accggaccac tggctgggct
     8761 ggtgatgagt tgggcgggcg tcccggtgat ttatctggcg gcggcgggac tggtcgcaat
     8821 cgcgttatta ctgacgtggc gattaaaaaa acggcctccg gaacacgtcc ctgaggccgc
     8881 ctcatcatct taaaacttac tgaataacca gcgtattaat gatgttttct gcggtggtct
     8941 gcgctttttg ctgattgtca gctggcagcg taatttgcat ggtcagcagt tgattaccca
     9001 cgttacccag aataacggaa gagtacgccg tctggccttt cgcggagata atgctgtcta
     9061 actgctgcat tttgtgacct ttcagctcaa tggctttatt ggttaccact tgcagctgcg
     9121 gatcgcggct acgttgctga tcttccagac gcttcgccaa caccgccaga tcttctttcg
     9181 gatcatcgcc catgatgacg atgactgctt tttgcccagt ggcgtcggac cagacatgca
     9241 tgttattggc ctgcgttccc agcttaccgc tctggtcggt catatccgct ggcagcgaga
     9301 aacttaactt gccatcaagc aggttgacgg gattcccggt agcgttactt tccgcgaccg
     9361 acccctgcgc cgtagcgtta gtgtctttat catcacaggc cgcaagcccc ataaccagca
     9421 ggccaattcc gacatattta accagattgc gcattgactt cttcctttcg ataaacggcc
     9481 ataacggctc attcatccat cttatcacaa ctctgataac gaacctttaa ctcgcctgca
     9541 aagcgttgat ttcagattta tctgccagtc ttttcaacag catattgagt aatacgccat
     9601 acattggcag gaagaaaacg atactgatta acactttgaa acagtaatcg accagcgcga
     9661 tttccatcca gtgttcagcc ataaaggcat ccgggctacg ccagaaggca atgaagaaga
     9721 aggccagcgt gtcgctgacg ttaccgaaca gtgtggacgc tgtcggtgcc agccaccagt
     9781 gacgactctg acgcaggcgg ttaaaaacgt gcacgtcgag gatctgcccc agcgcgtagg
     9841 ccatgaaact ggcggtggcg atacgggcga caaacaggtt gaagtgggcg agcgcgccga
     9901 atccctgcca ggaacccata tagaacagcg acgagatgac gtaggagatc agtaacgccg
     9961 ggatcatcac cgcaaagatg atacgacggg ccagcggtgc gccaaaaata cgcacggtca
    10021 ggtcggtagc aaggaaaata aacggaaagc taaatgcgcc ccaggtggta tggaaaccaa
    10081 aaatggagac gggaagctga accagatagt tactggaggt gatcaccagc agatgaaata
    10141 acgataacca gaacaacgcc ttatagcgtt gagtttgcga gaaaacgttc atattgtacc
    10201 tttttgatta accattgggg tgagggaacc caatacgtac gacacgctat tttaccttga
    10261 ttaaataacg ttcttaccct tttttcgagc cgccgcatga tactgcttta cgttgacaat
    10321 gcaatggtta attttcacgc aatcgttaac ctggtttgct tacggactca cagggcgtaa
    10381 actagcgccg tttttttatg tgatgagaag aaaatgaccg atctcttttc cagccctgac
    10441 cacacactcg acgcacttgg cctgcgctgc ccggaaccag taatgatggt gcgcaaaaca
    10501 gtgcgcaata tgcagccagg cgaaacgttg ctgattatcg ccgacgatcc ggccactacc
    10561 cgcgatattc ctgggttttg tacctttatg gaacacgaac tggttgctaa agagacggat
    10621 gaactgcctt atcgttattt gattcgcaaa ggcggttgat ggggaaagat tggcttcgat
    10681 gccgcctttt cccctcaccc taaccctctc cccagaggag cgaggggacc gatcgcgctc
    10741 aatgttgcga tcggtttgcc ttatctcctg cgcaacaatc ttaacgcatt cgctgtcacc
    10801 agcaccgtcg cacccgtatc tgccagcacc gccagccata gcccggtcat tcctaacagt
    10861 gtggtgacga ggaagatccc tttcagcccc agcgcaatag tgatgttctg gcggatattg
    10921 gcgtgagtgg cgcgtgccag ttcaatcatt tgcaccaggc cgcgcaggtg gttatgggtt
    10981 aatgctgcat cggcggtttc cagcgccacg tctgtgccac tgcccattgc aatcccgatg
    11041 gcggcggctt tcatcgctgg cgcgtcgtta ataccgtcac cgaccatcgc cagcggcgca
    11101 tgttgattca gctcggtcac cgctttgact ttatcttccg gcaacaggcc cgctttaaac
    11161 tctagcccca gttccccggc gattgccgcc gctgcgcgtg gattatcgcc ggtgaggatc
    11221 acccctttga cgcccagcgc gttcagttca ctgatggcag ttgcagcatc ggcgcgcagg
    11281 gtatcctgta acgcaatgac accaagcacg tcatcgttac gtactaccag cactaccgtt
    11341 tgcccggcgc tttccagttc gttaattaaa ccagcaaatg catcagcggg atgtttcccg
    11401 gcagcgcata tcaacacgcg ctcaccgtta acctgcgctt caatgccaga cccaaccagc
    11461 gcccgctgtg attgggcggc gggaatggcg agttcagcaa cctgtgcttc tcgcacgatg
    11521 gcttgcgcca gtggatgcgt cgcgccttgc tcgaccgccg ctgccagtgt cagcagttcg
    11581 gattcactaa tacccgttgc cggatgaatc gcggtaacgc gcggtttacc gacggtcagc
    11641 gtaccggttt tatcaaacgc cacctgggta acacgaccca gttgttccag cgccgctccg
    11701 cctttaatta acgccccacg acgcgctgct gctgccagcc cggaggtaat cgccgcaggc
    11761 gttgagataa ctaacgcaca cgggcagcca atcagcagca gcgtcagccc tttataaatc
    11821 cactcttgcc agctggcggc aaacagcagt ggtggcacca gcgttaccag cagagcgacg
    11881 gccataatcg ctggcgtgta aatacggctg aaacggtcga taaaccgctc aatgggagcg
    11941 cgacgctctt cggcttcttc gatcagtttc agaatccggt caatggcgct ggcacccggt
    12001 tctgacagca cttccagcat taccagacgg tctacgctgg tggcgcctgc aggaacttta
    12061 tcgcccgtcg cgcgctccac aggaatggat tctccggtca gggcgctttc atcaaaactg
    12121 gcaaacggtg agagcagttt accgtcggca ggcaaacgcc cacctgccgc aacttcaatc
    12181 acatcgccag gccgcaggct gtttattgcc acctcttcac gctcaccgtt acgcagacgc
    12241 gtggcggttt ctggcttcag cgccattaac gcgctgaccc cttgacgcgc gcgactggcg
    12301 gcccagccct ccagtcgttc gccaatcaaa aacagcagca acaccatcgc agcttcagcc
    12361 gttgcgccaa taaacagcgc accaatagcg gctacgctca ttaacgtttc aatggcgaag
    12421 tagctgccgg atttgatcag ccgtaatgcc tgacgagcaa tcgggtacag cccaaccagc
    12481 gtggtcgcga taaacgccag ttgcccgaac ggatgattga actgctccag accccagctg
    12541 attgccatca tcacgattag cgtaatcagc ggcagattct ctttcaggcg tgatgcttgt
    12601 ggttcgtcgg cggactgttc atcgcgcagg gaatagcctg ctttttgcac cgcagattca
    12661 acctgggcac ggatgtcatt gtcggcatcg accaccagtt tttcggtggc gaacaacacc
    12721 tgcacctgat tcacgcctgc aagctggcgc acggcatttt ctaccttgcg cgcacaggcg
    12781 gcgcagtcca tgccgctgac tttccagcta tagcgggtgc tggagacgtt ttcagagaga
    12841 gttggcgtgc tggaacatgc gccgtcgcag caacagtcgt tggcgttctg taccgtggtt
    12901 agcggtttga acgcagcaaa ttgaggggct ttcttgccgt gattgtcagg agtcgacatg
    12961 gcatcctccg gttaagtttt ttctcattaa ccgaaggata cactctggag tcgactccag
    13021 agtcaagttt tatcagagat acagcgagcg gacgatgagg aaatgaccgg caaagtagca
    13081 ggccgcagca atcgcgttat ctgcccggaa gcggcggcga tagtggctac ccagccagac
    13141 aaagttactg ataaacagca acgaagcgcc gacaaacgca gagagcgccg gagctgtcgg
    13201 acggaagaac cacagttcac ctgccagcca caccatcacc agcgtcatgc cgataaacgt
    13261 gcagataggc caacgatact cttccagacg cgtccagata atcgccagta acagcgcacc
    13321 cagaaccagc aacaccagtg gcagaggcca gaagaaagag agcgtcatct gactggcaaa
    13381 atagatggtg tacagcaggt gcgagaggaa aaacgcgccg atggcgtaca tcagacgttg
    13441 acgtggcaac agggttagcg catcgcccag cagtgaggcg cacagccctg ccagcaccag
    13501 atagctaatg gcgtcgaaca tcggcgcttg ccaggccagt aacagcagga gaagaagggt
    13561 taacggttta aacacccagc gttgccaggt tggcccacga tacgatgcat ccacagatag
    13621 ccatgcggaa agacagacag cgataaacga ccaaagcatc ttagttcctt gaattcgtta
    13681 ttttttcgta agcggaatgc ctacggtctt gcttccagtt tagtggcgag ggtggacgga
    13741 tgacaacacc ataactggca gggtatgctt atttccgcat tttccgatga gggataaaga
    13801 tgagcaaacc acctcttttc tttattgtta tcattggctt aattgtcgtc gccgcatcgt
    13861 ttcgttttat gcaacagcga cgggaaaaag ctgataatga tatggctccg ctccagcaaa
    13921 agctggtggt ggtgagcaac aagcgggaaa aaccgattaa cgatcgccgt tcgcgccagc
    13981 aggaagtgac tccggcaggc accagtatgc gctacgaggc aagcttcaaa ccgcaaagcg
    14041 gaggaatgga gcagacgttt cgcctcgacg ctcagcagta ccatgccctg acagtgggcg
    14101 atataggtac gctgagctat aaaggaacgc gctttgttag ctttgtaggc gaacaataag
    14161 gttatttttt aactttaaat ttcttctgcc agaccagcag ttcaaacacg ccgaaaagga
    14221 aaatccgcac cttttcggcg gtggtcatct gcgggccatc tttaggtaaa gtggatttca
    14281 atagcgccag ctgcatacca tgcatcagca cggtaaaaat cagcgccacg ttaacgaaaa
    14341 tattcagcgg gcgcgggaag ggatgcacca gattgaggat taaaaatccc caaacgcaga
    14401 gcattaacaa acgaccaata ttaatcagca tcactttctc cttgtgcttc gcgttgatac
    14461 agccgataag ccacctgacc cgccactttt tcccgatgta atgaccagtt tgccggaaca
    14521 gtgggcagac cgttttcgac ttcgctttcg acataaatca gggcttcgtc agccagccag
    14581 ccgttatctt ccagtaaatt tatcgtctct tctaacaagc cacggcggaa cggtggatcg
    14641 acaaacacga tattatgcgg tgtgcctttt tgcgccagga atgacattgc gttgctgttc
    14701 accacgcgtg cattgcctgc ttttagtgtc gccagattct taattaactg ctgagaaacc
    14761 gcgcgatcca tctcaatcaa cgtggccccc gcagcatagc gcgacaacgc ttccagcccc
    14821 agcgcgccgc tcccggcgaa gcaatccaga cattgggcgt caacaatgac cggagccagc
    14881 cagttaaaca acgtttcgcg tacgcggtcg gtggtggggc gcagacctgg gctatcagga
    14941 accgggagtt tacggcctcg ccactgcccg ccaataatgc ggatttggcc gctgccggaa
    15001 tgattcggtt ttttcatgat aaattgctca atccgccaga taacacatac ttgcaggcgg
    15061 tgatgtgaat taagttaagt gatagactat ttcatcattt ttttagctgc tatgtacata
    15121 gcgttaacgc tgtgccatga agcaacagcg aggagtgtag tcgcaaatgg cgaaagaaaa
    15181 aaaacgtggc tttttttcct ggctgggctt tggtcaaaaa gagcagaccc cggaaaaaga
    15241 gacagaagtt cagaatgaac aaccggttgt agaagaaatc gttcaggcgc aagagcctgc
    15301 gaaggcttcg gaacaagccg ttgaagagca gccgcaggcg catactgagg ccgaagcgga
    15361 aacttttgct gccgacgttg tggaagtcac tgaacaggtt gttgaaagtg aaaaagcgca
    15421 acctgaagcg gaagttgttg cacagccgga actggtcgta gaagaaacgc cggagccagt
    15481 ggctatcgag cgtgaagagc tgccgttgcc ggaagacgtc aacgccgaag aggtttcgcc
    15541 agaagagtgg caggccgaag cggaaaccgt agagattgtc gaagcggcgg aagaagaagc
    15601 ggctaaagaa gaaatcaccg acgaagagct ggaagcacag gcgctggctg ccgaagcagc
    15661 agaagaggcg gtgatggtgg ttcctccggc agaagaagag cagccggtag aagaaatcgc
    15721 tcaggagcag gaaaaaccga ccaaagaagg tttcttcgcg cgcctgaaac gcagcctgtt
    15781 aaaaactaaa gaaaatctcg gttccggatt tatcagcctg ttccgcggta aaaaaatcga
    15841 cgatgatctg tttgaagagc tggaagaaca gctgttgatc gccgatgtgg gcgtggaaac
    15901 cacacgtaaa ataatcacca atctgacgga aggcgcatcc cgcaagcagc ttcgtgatgc
    15961 cgaggcgctt tatggcctgc tgaaagaaga gatgggcgag attctggcga aagtcgatga
    16021 gccgctgaat gttgaaggta aaacgccgtt cgtgatcctg atggtgggcg tcaacggtgt
    16081 gggtaaaacc acgacgattg gtaagctggc acgtcagttt gagcagcagg gtaaatcggt
    16141 gatgctggcg gcgggcgata ctttccgtgc ggcagcggtt gaacagcttc aggtctgggg
    16201 tcagcgcaac aatattccgg tgattgccca gcataccggg gcggattccg cctctgttat
    16261 cttcgacgcc attcaggcag ctaaagcgcg taatatcgac gtcctgattg ccgataccgc
    16321 cggacgcctg cagaacaaat cgcacctgat ggaagagttg aagaaaatcg tccgcgtgat
    16381 gaagaaactc gacgttgaag cgccgcatga agttatgctg actattgatg ccagcaccgg
    16441 gcagaacgcg gtaagccagg ccaaactgtt ccatgaagcc gttggcttaa ccggcatcac
    16501 gctaacgaaa ctggacggca cggcgaaagg cggagtaatt ttctcggtgg ctgaccagtt
    16561 tggtatccct atccgctaca ttggtgtcgg cgaacgtatt gaggatttgc gtccgtttaa
    16621 ggcggacgac tttatagagg cactttttgc ccgagaggat taacaatgat tcgctttgaa
    16681 catgtcagca aggcttatct cggtgggaga caggcgctgc agggcgttac gttccatatg
    16741 cagccgggtg agatggcgtt tctgaccggt cattccggcg cagggaaaag taccctcctg
    16801 aagctgatct gtgggattga gcggcccagc gccgggaaaa tctggtttag cggccatgac
    16861 atcacgcgtc tgaaaaaccg tgaagttccg tttctgcgcc gccagattgg tatgattttc
    16921 caggatcacc atctactgat ggaccgtact gtctacgata acgtggcgat cccgctgatt
    16981 atcgccggtg ccagcggtga cgatattcgt cgccgggtgt cggcggcact ggataaagtc
    17041 gggctactgg acaaagcgaa gaactttcct attcagcttt ccggcggtga acaacagcgt
    17101 gttggcattg cccgcgcggt ggtgaataag cccgcggtac tgctggcgga cgaaccgact
    17161 ggtaacctgg acgacgcgtt gtcggaaggc attttacgtc tgtttgaaga gtttaaccgc
    17221 gttggggtaa ccgtattgat ggcaacgcac gacatcaacc tgatttcgcg gcgttcctat
    17281 cgcatgctca ccctgagcga tggtcacttg catggaggcg tgggccatga ataagcgcga
    17341 tgcaatcaat catattcggc agtttggcgg gcgtctggat cgcttccgta aatcggtcgg
    17401 cggctcaggc gacggcggtc gtaacgcacc gaaacgcgcg aaatcctcgc caaaaccggt
    17461 aaatcgcaaa accaacgttt tcaacgaaca ggtgcgctat gccttccacg gcgcattgca
    17521 ggatctgaaa agcaaaccgt tcgccacgtt tttaacggtg atggttatcg ccatctccct
    17581 gacgctgccc agcgtctgtt atatggtgta caaaaacgtt aaccaggcgg cgacgcaata
    17641 ttatccgtca ccgcaaatca ctgtttatct gcaaaaaacg ctggacgatg acgccgctgc
    17701 gggtgtggtg gcacagttac aggccgagca aggcgtagag aaagtgaatt atctttctcg
    17761 tgaagacgca ctgggcgagt tccgtaactg gtctggtttt ggtggtgcgc tggatatgct
    17821 ggaagaaaac ccgcttccgg cagtggcggt ggtgatcccg aaactcgatt ttcaggggac
    17881 ggaatcactg aatacgctgc gtgatcgtat cacccagatc aacggcattg acgaagtgcg
    17941 gatggatgac agctggtttg cccgtctggc ggcgttaacc gggctggtcg ggcgcgtttc
    18001 cgcgatgatc ggcgtgttga tggtggcagc agtgttcctc gtcatcggca acagtgtgcg
    18061 tctgagcatc tttgctcgcc gtgactccat taacgtgcag aaactgattg gtgcgacgga
    18121 tggtttcatc ctgcgcccgt tcctgtacgg aggcgcattg ctgggatttt ctggcgcatt
    18181 gctgtcatta attttgtcag aaattctggt actgcgtttg tcatcggcgg ttgcggaagt
    18241 ggcacaggtt ttcggaacaa agtttgatat caatggctta tcgttcgatg aatgcctgct
    18301 gttgctgctg gtatgctcga tgattggctg ggtggcagcg tggcttgcca cggtacaaca
    18361 tttacgccac tttacgccgg aataataaaa gcgtgttata ctctttccct gcaatgggtt
    18421 ccgtagcagg gaaagagacc ccgttgtctc ttcccggtat ttcatctcta tgtcacattt
    18481 tgtgcgtaat ttattcacaa gcttgcattg aacttgtgga taaaatcacg gtctgataaa
    18541 acagtgaatg ataacctcgt tgctcttaag ctctggcaca gttgttgcta ccactgaagc
    18601 gccagaagat atcgattgag aggatttgaa tgactgacaa aatgcaaagt ttagctttag
    18661 ccccagttgg caacctggat tcctacatcc gggcagctaa cgcgtggccg atgttgtcgg
    18721 ctgacgagga gcgggcgctg gctgaaaagc tgcattacca tggcgatctg gaagcagcta
    18781 aaacgctgat cctgtctcac ctgcggtttg ttgttcatat tgctcgtaat tatgcgggct
    18841 atggcctgcc acaggcggat ttgattcagg aaggtaacat cggcctgatg aaagcagtgc
    18901 gccgttttaa cccggaagtg ggtgtgcgcc tggtctcctt cgccgtccac tggatcaaag
    18961 cagagatcca cgaatacgtt ctgcgtaact ggcgtatcgt caaagttgcg accaccaaag
    19021 cgcagcgcaa actgttcttc aacctgcgta aaaccaagca gcgtctgggc tggtttaatc
    19081 aggatgaagt tgaaatggtg gcccgtgaac tgggcgtaac cagcaaagac gtacgtgaga
    19141 tggagtcgcg tatggcggca caggacatga cctttgacct gtcttccgat gacgattccg
    19201 acagccagcc gatggctccg gtgctctatc tgcaggataa atcatctaac tttgccgacg
    19261 gcattgaaga tgataattgg gaagagcaag cggcaaaccg tctgaccgac gcgatgcaag
    19321 gtctggacga gcgtagccag gatatcatcc gcgcgcgctg gctggatgaa gacaacaagt
    19381 ccacgttgca ggaactggct gaccgttacg gtgtttccgc tgagcgtgtg cgccagctgg
    19441 aaaagaatgc gatgaaaaaa cttcgcgctg ccattgaagc gtaatttccg ctactcagca
    19501 gagaaccctg gatgagaatc cggggttttt gttttttggg cctctgcaat aatcagtttc
    19561 cccctccggc aaaacgccaa tccccacgca gattgttaat aaactgtcaa aatagctatt
    19621 ccaatatcat aaaaatcggg tatgttttag cagagtatgc tgctaaagca cgggtagtca
    19681 tgcataaaac gaaataaagt gctgaacaac aacatcacaa cacacgtaat aaccagaaga
    19741 atggggattc tcaggatgaa cataaagggt aaagcgttac tggcaggatg tatcgcgctg
    19801 gcattcagca atatggctct ggcagaagat attaaagtcg ccgtcgtagg cgcaatgtcc
    19861 ggtccggtgg cgcagtacgg tgaccaggag tttactggcg cagagcaggc ggttgcggat
    19921 atcaatgcta aaggtggtat caaaggcaac aaactgcaaa tcgtaaaata tgacgatgcc
    19981 tgtgacccga aacaggcggt tgcggtggcg aacaaagtcg ttaacgacgg cattaaatac
    20041 gtgattggtc acctctgctc ttcatcaacg cagccagcgt ctgacatcta cgaagacgaa
    20101 ggcattttaa tgatcacccc agcggcaacc gcaccagagc tgaccgcccg tggctatcag
    20161 ctgatcctgc gcaccaccgg cctggactcc gaccaggggc cgacggcggc gaaatatatt
    20221 cttgagaaag tgaaaccgca gcgtattgct atcgttcacg acaaacagca atacggcgaa
    20281 ggtctggcgc gagcggtgca ggacggcctg aagaaaggca atgctaacgt ggtgttcttt
    20341 gatggtatca ccgccgggga aaaagatttc tcaacgctgg tggcgcgtct gaagaaagag
    20401 aatatcgact tcgtttacta cggcggttat cacccggaaa tggggcaaat cctgcgtcag
    20461 gcacgcgcgg cagggctgaa aactcagttt atggggccgg aaggcgtggc taacgtctcg
    20521 ctgtctaaca ttgcgggtga atcagcggaa ggactgctgg tgaccaagcc gaagaactac
    20581 gatcaggttc cggcgaacaa acccattgtt gacgcgatca aagcgaagaa acaggaccca
    20641 agcggcgcgt tcgtctggac cacctacgcc gcgctgcaat ctttgcaggt gggcctgaat
    20701 cagtctgacg atccggctga aatagccaaa tacctgaaag cgaactccgt ggatacggtg
    20761 atggggccgc tgacctggga tgagaaaggt gatctgaaag gctttgagtt cggcgtattt
    20821 gactggcacg ccaacggcac ggcgactgat gcgaagtaat cattaatcgg caactttggg
    20881 ttgccgccaa attgctaata tcgagtacgt tgcttcatgc cggatgcggc gtaaacgcct
    20941 tatccggcct acaagattca aagaaatcag taaattgcag cacacattgt aggcctgata
    21001 agcgtagcgc atcaggcaat atcacttgtg aaatcaaact tgacgattaa cgcttctccc
    21061 agccgccctg ttgtgccgta aatcccagtg cctgcataaa cgctgtcatc acgccgcgat
    21121 cttccacgcc tgcatccgcc atccaccagc atgaaacgcc aggattgtta cgcaaaacct
    21181 cttccagcag atattgcccc acaccgcgac ggcgggtgac ttccctcaca cgcagggaat
    21241 ccagtgcccc ctcggtgccg cttaaggtta cccgcacggc cgcgagaagg cgctcgttaa
    21301 aacgcgcggc gtagatacgg tggttatcgt caacctgtaa agaggaaggg gaatactccg
    21361 gccagatctt ttgcaggtca atccggtcct ggtcgctaaa gttttctaat cgaatgatgg
    21421 tcagcttcat ggggaacccg tgtaaatcac aaaagtggaa ccagtgtagc gaaataattt
    21481 aatcggaggc tttctctttt ttaattcttt tggcaggtga ttaatttttt aacagcaata
    21541 attacaaagt taaaacatta gagaatgaaa aatacccagc ataatcccct gaatggtagt
    21601 gaattattcc gccctttgta ccgttatttt atgctgacaa cggcgctttt ttctgtttat
    21661 ctatcaataa attcagaata ttatctgttc ttaatcgact gaaaaatagg gattttaatc
    21721 gctatcatca caaaatactg tgctaaaccc ttaatcagac aggcaaaaac agtgcagtat
    21781 aaaaaaagaa cagtctgatt tgttaacaca taagaacaaa gcaacacaac atcacgaatg
    21841 gggatttttg actatgaaac ggaatgcgaa aactatcatc gcagggatga ttgcactggc
    21901 aatttcacac accgctatgg ctgacgatat taaagtcgcc gttgtcggcg cgatgtccgg
    21961 cccgattgcc cagtggggcg atatggaatt taacggcgcg cgtcaggcga ttaaagacat
    22021 taatgccaaa gggggaatta aaggcgataa gctggttggc gtggaatatg acgacgcctg
    22081 cgacccgaaa caagccgttg cggtcgccaa caaaatcgtt aacgacggca ttaaatacgt
    22141 tattgggcac ctgtgttcct cctctaccca acctgcatca gatatctacg aagacgaagg
    22201 tattctgatg atttcgccgg gagcgaccaa tccggagctg acccaacgcg gttatcaata
    22261 catcatgcgt actgccggtc tggactcttc ccaggggcca acggcggcaa aatacattct
    22321 tgagacggtg aagccccagc gcatcgccat cattcacgac aaacaacagt atggcgaagg
    22381 gctggcgcgt tcggtgcagg acggattgaa agcagccaat gccaacgtcg tcttcttcga
    22441 cggcattacc gctggtgaga aagatttctc cgcgctgatc gcccgcctga aaaaagaaaa
    22501 catcgacttc gtttactacg gcggttacta cccggaaatg gggcagatgc tgcgccaggc
    22561 ccgttccgtt ggcctgaaaa ctcagtttat ggggccggaa ggggtgggta atgcgtcgct
    22621 gtcgaacatt gctggtgatg ccgccgaagg tatgttggtc actatgccaa aacgctatga
    22681 ccaggacccg gcaaacaaag gcatcgttga tgcgctgaaa gcagacaaga aagatccgtc
    22741 cggaccttat gtctggatca cctacgcggc ggtgcaatct ctggcgactg cccttgagcg
    22801 taccggcagc gatgagccgc tggcgctggt gaaagattta aaagctaacg gtgcaaacac
    22861 cgtgattggg ccgctgaact gggatgaaaa aggcgatctt aagggatttg attttggtgt
    22921 cttccagtgg cacgccgacg gttcatcgac ggcagccaag tgatcatccc accgcccgtg
    22981 aaatgcgggc ggttttagaa aggttacctt atgtctgagc agtttttgta tttcttgcag
    23041 cagatgttta acggcgtcac gctgggcagt acctacgcgc tgatagccat cggctacacc
    23101 atggtttacg gcattatcgg catgatcaac ttcgcccacg gcgaggttta tatgattggt
    23161 agctatgtct cctttatgat catcgccgca ctgatgatga tgggcattga taccggctgg
    23221 ctgctggtgg cggcgggatt cgtcggcgca atcgtcattg ccagcgccta cggctggagt
    23281 atcgaacggg tagcctatcg tccggtgcgt aactctaagc gcctgattgc gcttatttcc
    23341 gcaatcggca tgtctatctt cctgcaaaac tacgtcagcc tgaccgaagg ttcgcgcgac
    23401 gtggcgctgc ccagcctgtt taacggtcag tgggtggtgg gccatagcga aaacttctct
    23461 gcctctatta ccaccatgca ggcggtgata tggattgtta ctttcctcgc catgctggcg
    23521 ctgacgattt tcattcgcta ttcccgtatg gggcgcgcct gccgtgcctg cgcggaagat
    23581 ctgaaaatgg cgagcctgct tggcatcaac accgaccggg tgattgcgct gacctttgtg
    23641 attggcgcgg cgatggcggc agtggcgggc gtgctgctcg gtcagttcta cggcgtaatt
    23701 aacccttaca tcggctttat ggccgggatg aaagccttta ccgctgcggt gctcggtggg
    23761 attggcagca ttccgggggc gatgattggc gggctgatcc tggggattgc ggaggcgctc
    23821 tcttctgcct atctgagtac ggaatataaa gatgtggtct cattcgccct gctgatcctg
    23881 gtgctgctgg tgatgccgac cggtattctg ggtcgcccgg aggtagagaa agtatgaaac
    23941 cgatgcatat tgcaatggcg ctgctctctg ccgctatgtt ctttgtgctg gcgggcgtct
    24001 ttatgggcgt gcaactggag ctggatggca ccaaactggt ggtcgacacg gcttcagaga
    24061 tccgttggca gtgggtgttt atcggcacgg cggtggtctt tttcttccag cttgtgcggc
    24121 ctgctttcca gaaagggcta aaaagcgttt ccgggccgaa gtttattctg cccgcgattg
    24181 atggctccac ggtgaagcag aaactgttcc tcgtggcgct gctggtgctt gcagtggcgt
    24241 ggccgtttat ggtgtcgcgt gggacggtgg atatcgccac tctgaccatg atctacatta
    24301 ttctcggtct cgggctgaac gtggttgttg gtctttctgg tctgctggtg ctgggatacg
    24361 gcagctttta cgccatcggc gcttacactt ttgcgctgct caatcactat tacggtctgg
    24421 gcttctggac ctgtctgccg attgccgggt tgatggcggc ggcggcgggc ttcctgctcg
    24481 gttttccggt gctgcgtctg cgcggtgact atctggcgat cgttaccctc gggttcggcg
    24541 aaattgtgcg catattgctg ctcaataaca ccgaaattac cggcggaccg aacggtatca
    24601 gtcagattcc gaaaccgacg ctcttcggac tcgagttcag ccgtaccgcc cgcgaaggcg
    24661 gctgggacac gttcagtaat ttctttggcc tgaaatacga tccctccgat cgcgtcatct
    24721 tcctctacct ggtggcgttg ctgctggtgg tgttaagcct gtttgtcatt aaccgcctgc
    24781 tgcggatgcc gctgggccgt gcgtgggaag cgttgcgtga agacgagatc gcctgccgtt
    24841 cgctgggctt aagcccgcgt cgtatcaagc tgactgcctt taccatcagc gccgcgtttg
    24901 ctggttttgc cggaacgctg tttgctgcgc gtcagggctt tgtcagcccg gaatccttca
    24961 cttttgccga atcggcgttt gtgctggcga tagtggtgct cggtggtatg ggttcgcaat
    25021 ttgcggtgat tctggcggca attttgctgg tggtgtcgcg agagttgatg cgtgatttca
    25081 acgaatacag catgttaatg ctcggtggtt tgatggtgct gatgatgatc tggcgtccgc
    25141 agggcttgct gcccatgacg cgcccgcaac tcaagctgaa aaacggcgca gcgaaaggag
    25201 agcaggcatg agtcagccat tattatctgt taacggcctg atgatgcgct tcggcggcct
    25261 gctggcggtg aataacgtca atcttgaact gtacccgcag gagatcgtct cgttaatcgg
    25321 ccctaacggt gccggaaaaa ccacggtttt taactgcctg accggtttct acaaacccac
    25381 cggtggcact attttactgc gcgatcagca tctggaaggt ttgcccggcc agcaaatcgc
    25441 ccgcatgggc gtggtgcgca ccttccagca tgtgcgtctg tttcgtgaaa tgacggtgat
    25501 tgaaaacttg ctggtggcgc agcatcagca actgaaaacc gggctgttct ccggcctgtt
    25561 gaaaacgcct tctttccgcc gcgcccagag cgaagcgctc gaccgtgccg caacctggct
    25621 tgagcgcatt ggtttgctcg aacacgctaa ccgtcaggcg agtaacctgg cctacggtga
    25681 ccagcgccgt ctggagatcg cccgctgcat ggtaacgcag ccggagattt taatgctcga
    25741 cgaaccggca gcaggtctta acccgaaaga gacgaaagag ctggatgagc tgattgccga
    25801 actgcgtaac catcacaaca ccactatctt gttgattgaa cacgatatga agctggtgat
    25861 ggggatttca gacagaattt acgtggtcaa tcaggggacg ccgctggcaa acggtacgcc
    25921 ggagcagatc cgtaataacc cggacgtgat ccgtgcctat ttaggtgagg cataagatgg
    25981 aaaaagtcat gttgtccttt gacaaagtta gcgcccacta cggcaaaatc caggcgctgc
    26041 atgaggtgag cctgcatatc aatcagggcg aaattgtcac gctgattggc gcgaacgggg
    26101 cggggaaaac caccttgctc ggcacgttat gcggcgaccc acgcgccacc agcgggcgaa
    26161 ttgtgtttga tgataaagac attaccgact ggcagacggc gaaaatcatg cgcgaagcgg
    26221 tggcgattgt cccggaagga cgtcgcgtct tctcacgcat gacggtggaa gagaatctgg
    26281 cgatgggcgg cttttttgct gaacgcgacc agttccagga gcgcataaag tgggtgtatg
    26341 aactgtttcc acgtctgcat gaacgccgtg ttcagcgggc aggcaccatg tccggcggtg
    26401 aacagcagat gctggcgatt ggtcgcgctt tgatgagtaa cccacgtttg ttattgcttg
    26461 atgaaccctc gctcggactg gcaccgatta tcatccagca aattttcgac accatcgagc
    26521 aactgcgcga gcaggggatg accatcttcc tcgtcgagca gaacgccaac caggcgctga
    26581 agctcgctga tcgcggctac gtgctggaaa acggtcatgt ggtgctctcc gatactggcg
    26641 atgcgctgct ggcgaacgaa gcggtgagaa gtgcgtattt aggcgggtaa taatattttg
    26701 agtaaaaagg agtcgaatga ctcctttttt gtacttgtta gttgtacaat ttaattgtac
    26761 ttaatatgga ggatatcatg cataccatga actacagcga agcgcggcag aaccttgcat
    26821 tagttctgga aacagcggcg acaggtactc cggtaacgat cacgaggcgt gggcataagg
    26881 cagctgtgat tatcagcgca gaagagtttg aacgttacca gaaggctaaa ctggacgcag
    26941 aatttgacgc catcatggct attcatggca gcgaaattaa ggagttagcg gataaatgac
    27001 cattcagttt atctcagcgg aggagataat ttatttccac gataaattgc ttagcgttac
    27061 accaggtgta acaggaatgt ccgatccagg gcgagcagaa gcgcttttgt accgggtact
    27121 gaataagtat gagtatgaag gcgtttctga tctttggctg ctggcggcga tgcatctatt
    27181 ggcaatctcg cgcggtcata tctttaatga tggtaataag cgcaccgcac tgtttatcac
    27241 gctattattc cttaaacgta acggcatcat acttgctgca aacccagagt ttgtagaatt
    27301 gacggttgat gtggcggcgg gaaaagtatc gttagaagat atcgttaccc gcttgcgtca
    27361 acgcggtacc tgacaaggtt cttagccatt ctgcttttta tccgtgcccc atctcccatt
    27421 ttccctcacc cacgccgtca ccgccttgtc atctttctga caccttacta tcttacaaat
    27481 gtaacaaaaa agttattttt ctgtaattcg agcatgtcat gttaccccgc gagcataaaa
    27541 agcgtgaatt cgcgcatccg gtacaacaag agagataaac gatgaaaccg ttacattata
    27601 cagcttcagc actggcgctc ggactggcgt taatggggaa tgcacaggca gtgacgacca
    27661 ttccgttctg gcattctatg gaaggggaac tgggtaaaga ggtggattct ctggcccaac
    27721 gttttaacgc cgaaaacccg gattacaaaa ttgtaccgac ctataaaggc aactacgaac
    27781 agaatttaag cgcggggatt gccgcatttc gtaccggcaa cgcgccggct attttgcagg
    27841 tttatgaagt tggcaccgcc accatgatgg cgtcgaaagc cattaaaccg gtatatgacg
    27901 tgtttaaaga ggcggggatt cagttcgatg agtcgcagtt tgtgccgacg gtttcaggct
    27961 actactccga cagcaaaacc ggccacttac tctcccagcc gttcaatagc tcgactcctg
    28021 ttctctatta caacaaagac gccttcaaga aagccggttt agacccggaa cagccgccga
    28081 aaacctggca ggatctggcg gactatgccg cgaaactgaa agcctccggt atgaagtgcg
    28141 gctacgccag cggctggcag ggctggatcc aactggaaaa ctttagcgcc tggaacggtc
    28201 tgccgtttgc cagcaaaaac aacggctttg acggcacaga cgcggtgctg gagttcaaca
    28261 agccggagca ggtgaaacac atcgctatgc tcgaagagat gaacaagaag ggcgatttca
    28321 gctacgtcgg gcgtaaggat gaatccactg agaagttcta taacggtgat tgcgcgatga
    28381 ccaccgcctc ttccggttcg cttgccaaca ttcgtgagta cgccaaattt aactacggcg
    28441 taggcatgat gccttacgac gccgatgcga aagatgcacc gcaaaacgcc attatcggcg
    28501 gggccagcct gtgggtgatg cagggcaaag ataaagaaac gtataccggc gtggcgaagt
    28561 tcctcgactt cctcgcgaag ccagaaaacg ctgccgagtg gcatcagaaa accggttatc
    28621 tgccaatcac caaagcggcg tatgacctga cccgtgagca gggcttttat gagaaaaacc
    28681 caggggcgga taccgcgacg cgtcagatgc tgaataagcc gccgttaccg ttcaccaaag
    28741 ggctgcgtct gggcaacatg ccgcagatcc gcgtgattgt ggatgaagag ctggagagcg
    28801 tgtggaccgg taagaagaca ccgcagcagg cgctggatac cgccgttgag cgtgggaacc
    28861 agttactgcg ccgctttgag cagtctacga agtcttaatt ctcttaccct caccctaacc
    28921 ctctcccaaa gggagagggg accgatcggc gtggtatcct ggtgccgtgt cggtttgcaa
    28981 cttcgctcgg tgggaaagta tgcaccgcct ttctctctcg cccctctggg gagagggccg
    29041 gggtgagggg aacacaacac gaagagttta actatgtcat catcccgtcc ggtgttccgc
    29101 tcgcgctggc tgccctattt gctggtcgcg ccgcagctca tcatcaccgt tatctttttt
    29161 atctggcctg cgggcgaagc gttgtggtac tcgctgcaaa gcgtcgatcc gtttggtttc
    29221 tccagccagt ttgtcggcct ggataacttc gtcacgctgt ttcatgacag ctactatctc
    29281 gactccttct ggacgacgat aaaattcagc acctttgtca ccgtcagcgg tttgctggtg
    29341 tcgctgttct ttgcggcgct ggtggagtac atcgtgcgcg gcagccgttt ctatcaaacc
    29401 ttaatgctgc tgccttacgc tgtggctccc gccgttgccg ccgtattgtg gatcttcctg
    29461 tttaaccccg gtcgcgggct gatcacccat tttctcgctg agttcggcta cgactggaac
    29521 cacgcgcaaa acagcggtca ggcgatgttt ctggtggtgt ttgcctcggt gtggaagcaa
    29581 atcagctaca actttctgtt cttctatgcc gcgctgcaat ccattccccg ttcgttgatc
    29641 gaagccgcag ccatcgacgg tgcaggcccg attcgccgct tctttaagat tgcgctgccg
    29701 cttatcgctc cggtgagttt cttcctgctg gtggtgaacc tggtgtacgc cttcttcgat
    29761 accttcccgg tgatcgacgc cgccacgtcc ggcgggccgg ttcaggccac cacgacgctg
    29821 atttataaga tttaccgcga aggctttacc ggactggatc tggcttcgtc tgccgcgcag
    29881 tcggtggtgt tgatgttcct cgtcatcgtg ctgacggtgg tgcagttccg ctatgttgaa
    29941 agcaaggtgc gttaccaatg attgagaacc gtccgtggct gacaatattc agccatacca
    30001 tgctgatcct cgggatcgcg gtgatcctct tcccgctgta cgtggcgttt gtcgcggcga
    30061 cactggataa acaagccgtc tacgccgcgc cgatgacgct aatccccggc acgcatctgc
    30121 tggaaaacat ccacaacatc tgggtgaacg gggtaggcac gaatagcgcg ccgttctggc
    30181 ggatgttgct taacagcttt gtgatggcgt tcagcattac gctcggcaaa attaccgtct
    30241 cgatgctctc ggcctttgcc attgtctggt ttcgttttcc actacgtaac ctcttcttct
    30301 ggatgatttt tatcaccctg atgctgccgg ttgaagtgcg tatcttcccg acggtggaag
    30361 tcatcgccaa cctgaaaatg ctcgacagct acgcaggttt aacgctgccg ctgatggcct
    30421 cggcgaccgc cacgtttctg tttcgccagt tctttatgac cctaccggat gaactggtgg
    30481 aagccgcgcg gatcgacggc gcgtcaccaa tgcgtttctt ttgcgacatc gtttttccgc
    30541 tgtccaaaac caatctggcg gcgctgtttg tgatcacctt tatctacggc tggaaccagt
    30601 atttgtggcc gttgttgatt attaccgacg ttgatctcgg caccaccgtg gcagggatta
    30661 aagggatgat cgccacaggc gaaggcacaa cggaatggaa ctcggtgatg gcggcgatgt
    30721 tgttaaccct gatcccacca gtagtgattg ttttagtgat gcagcgtgcc ttcgtgcgcg
    30781 gcctggtcga tagtgagaaa taagatggca ggactgaaat tacaggcagt aaccaaaagc
    30841 tgggatggca aaacccaggt cattaaaccg ctgacccttg atgtggcgga tggcgaattt
    30901 atcgtgatgg tcgggccgtc tggctgcggg aaatcgacgt tattgcgaat ggttgccgga
    30961 ctggagcggg taacgacggg cgatatctgg atcgaccgca agcgtgtcac cgaaatggag
    31021 ccgaaagatc gcgggattgc gatggtgttc cagaactacg cgctttatcc gcatatgagc
    31081 gtcgaagaaa acatggcgtg ggggctgaaa attcgcggca tgggcaagca gcaaattgcc
    31141 gagcgcgtta aagaagcggc gcgcattctg gagctggacg gcctgcttaa gcgccgcccg
    31201 cgtgagctat ctggcggtca gcgtcagcgt gtggcgatgg ggcgtgcgat tgtgcgtgag
    31261 ccggcagtgt tcctgtttga tgagccgctc tctaacctcg atgccaagtt gcgtgtgcag
    31321 atgcgtcttg aactgcaaca gctgcaccgt cgcctgaaaa cgacttcact ctacgttacc
    31381 cacgatcagg ttgaggcgat gacgctcgcc cagcgagtaa tggtgatgaa cggcggcgtt
    31441 gccgaacaga ttggcacgcc agttgaagtg tacgaaaagc ccgccagcct gtttgtggcg
    31501 agttttatcg gcagtccggc gatgaatctg ctgaaagggc gtgtgaataa cgaaggtacg
    31561 catttcgaac tggacggcgg tatcgcgctg ccgctaaacg gtggctaccg tcagtatgct
    31621 ggacgtaaaa tgactctcgg cattcgcccg gaacatatta cgctacgttc gcaggctgaa
    31681 ggtggcgtgc cgctggtgat ggacacgctg gagatcctcg gcgcagataa cctggcgcac
    31741 ggacgctggg gcgaacagaa gctggtgttg cgactggcgc atcaggagcg cccgacggca
    31801 ggcagcacgc tgtggctgca tctgccggaa aatcagctac atctttttga tggtgaaaca
    31861 ggacaacgag tatgagtaac tggccttatc cccgcatcgt cgcccatcgt ggcggcggta
    31921 aactggcccc ggaaaacacc ctggcggcaa tcgatgtggg ggcaagatac ggtcataaga
    31981 tgatcgaatt tgacgcgaag ttatcgaaag atggcgagat cttcttgctt catgacgaca
    32041 atctcgaacg caccagcaac ggctggggcg tcgcgggtga actgaactgg caggatttac
    32101 tgcgcgtgga tgcgggcagt tggtacagca aagcgtttaa aggtgagccg ctgccgttgc
    32161 tttcgcaggt ggcggaacgc tgtcgcgaac acgggatgat ggcgaatatc gaaatcaaac
    32221 ccaccactgg caccgggcca ttaacgggca aaacggtggc gctggcggca cgcgaactgt
    32281 ggaccggtat gacgccgccg ctgctgtcat cgtttgagat tgatgcttta gaagccgcgc
    32341 aacaggcagc accggaactg ccgcgtggtt tattgcttga tgaatggcgc gacgactggc
    32401 gagaattgac cgcgcggctg ggctgcgtct ctattcatct caatcataag ttgctcgata
    32461 aagcgcgagt gatgcagttg aaagacgccg gattacggat tctggtttat accgtcaata
    32521 aaccccagcg cgcggcagag ttgctgcgct ggggcgtgga ttgcatctgt accgatgcga
    32581 ttgacgtgat tggtccgaac tttacggccc aatagttttc aacggaatgt caggctgcgg
    32641 cgtgctgggc tgattcagca tgtcgccgtt tctcctttcc ggcagcatat gctgctgatt
    32701 caacgaacta tccggattac ggttgctgtt taacatcccg ccattggtgt tgggcagcat
    32761 ttgctgtcgg gcgggatttc gctcccccgg ctgtgactgc aacacccgct gagaattatt
    32821 gtttatctgg ttttctaaat gctgctgttg cagctgcgtt tgcgttttca gttgctgatt
    32881 cagcatccct ttttgctgga tttgctgcgt ttgcatttgg gtttgcatcc gctgctgact
    32941 ggggatctga taccccggct ggttagggtt attcagagta ttaatgggct gtgcaaagcc
    33001 gacaaacggc aggagtgccg taagaatcag aagtcgtttc atcgcgtatc ctcctctgaa
    33061 gatattcttt aagtttactc ctttcctgca atcatcagga ttaattcaga gttatatacc
    33121 aggcttagct ggggttgccc cttaatctct ggagaataac gatgataaaa ccgacgtttt
    33181 tacgccgggt ggccattgct gctctgttca caggaagttg ttttagtacg gtagccgcgc
    33241 cacctgcgcc gtctcctgtt tcttacggcg tggaggaaga tgtctttcat ccggtacgcg
    33301 cgaaacaggg gatggtggcg tctgtagatg ctactgccac tcaggtgggg gtggatatcc
    33361 tcaaggaggg cgggaatgcc gttgatgccg ccgtggctgt gggctacgcg ctggcggtaa
    33421 cgcatccgca ggcagggaat ctgggcggtg gtggttttat gctgatccgc tcgaaaaatg
    33481 gcaataccac cgctatcgat ttccgcgaaa tggctcccgc caaagcgacc cgcgatatgt
    33541 tccttgatga tcagggtaac ccggacagca aaaaatcact cacttcgcat ctggcttctg
    33601 gtacgcccgg tacggtagca ggtttctcgc tggcgttgga taaatacggc accatgccgt
    33661 tgaacaaagt ggtgcaaccg gcgtttaaac tggcacgcga tggttttatc gttaacgacg
    33721 cgttggctga cgatctcaaa acctacggta gcgaagtgtt gccgaatcac gaaaacagca
    33781 aggctatctt ctggaaagag ggcgcgccgc tgaaaaaggg cgacaagctg gtgcaggcga
    33841 acctggcaaa gagcctggag atgattgccg aaaacggccc ggacgaattc tacaaaggca
    33901 cgatagcgga acagattgcc caggagatgc agaaaaacgg tggcttgatc actaaagaag
    33961 atttagcggc gtataaagcg gtcgaacgta caccgattag cggcgaatat cgcgggtatc
    34021 aggtttactc gatgccaccg ccatcttccg gtgggattca tatcgtgcag atcctcaata
    34081 tcctggaaaa cttcgatatg cagaaatacg gctttggcag cgccgatgcg atgcaaatca
    34141 tggcagaagc ggagaaatac gcctacgccg accgctcgga atatcttggc gatccggatt
    34201 ttgtcaaagt cccgtggcag gcgctgacca ataaagccta tgccaaatct attgccgatc
    34261 aaatcgatat caataaagcg aagccatcca gcgaaattcg ccctggcaag cttgcgcctt
    34321 atgagagtaa tcaaactacc cattactcag tggtggataa agatggtaac gcggtggcgg
    34381 tgacctatac gctgaacacc accttcggta cgggcattgt cgcgggcgag agcggtattc
    34441 tgcttaataa ccaaatggat gatttctccg ccaaaccggg cgtgccgaac gtttacgggc
    34501 tggtgggtgg tgacgctaac gcggtcgggc cgaataaacg tccgctgtcg tcgatgtcac
    34561 caaccattgt ggtgaaagac ggtaaaacct ggctggtcac cggaagccca ggcggtagcc
    34621 ggatcatcac taccgtgctg caaatggtgg tgaatagcat cgattatggt atgaatgtcg
    34681 ccgaagcgac caatgcaccg cgtttccacc atcagtggtt gccggatgag ctgcgtgtcg
    34741 agaaaggatt tagcccggat acgctcaagc tgctggaagc aaaaggtcag aaagtggcgc
    34801 tgaaagaggc gatgggcagt acacaaagca ttatggttgg gcctgacggt gagttgtacg
    34861 gcgcatccga cccgcgctca gtagatgatt taacggcggg gtattaaggt tagcggccct
    34921 cttcgtggga agagggccgt attgtcaggg caagccgaag gtagcctttt ttatttcata
    34981 atcctgtaga tattcttcca gcggctttgc cgttccaagg gaatgaattt caccactgtc
    35041 tttatcaata ataaaaggtg cgttaccggc taagcgcgcg gcctcatcac cagattcaag
    35101 aaattcttgt gattcgaaac agaaatacca gccctggata aaacgtccat gcagagtgat
    35161 gacgacaggg agatctgcat catcaaggta atggttcgct ttcgcgaatg cgtcgtgata
    35221 agtaatcata attataataa atattattgt tgagcgttat attattattc ttgcgttata
    35281 ctttgagaaa aggcgtgttc atcttcgtca aagatattat taaatgctgg ttttgaaaaa
    35341 agttcatcgg ataatataca gt
//
LOCUS       DAZGQL010000054        34914 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.054, whole genome shotgun sequence.
ACCESSION   DAZGQL010000054 DAZGQL010000000
VERSION     DAZGQL010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34914
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.054"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            41..2392
                     /gene="polB"
                     /locus_tag="ACHKIR_002839"
     CDS             41..2392
                     /gene="polB"
                     /locus_tag="ACHKIR_002839"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HFO9990549.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDIRNGAIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASALDFELEYVASRPQLLEKLNAWFATHDPD
                     VIIGWNVVQFDLRMLQKHAERYRIPLRLGRDNSELEWREHGFKNGVFFAQVKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGSIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            2556..5462
                     /gene="rapA"
                     /locus_tag="ACHKIR_002840"
     CDS             2556..5462
                     /gene="rapA"
                     /locus_tag="ACHKIR_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HFO9990550.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            5474..6133
                     /gene="rluA"
                     /locus_tag="ACHKIR_002841"
     CDS             5474..6133
                     /gene="rluA"
                     /locus_tag="ACHKIR_002841"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HFO9990551.1"
                     /translation="MGMENYNPPQEPWLVVLYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(6251..7066)
                     /gene="djlA"
                     /locus_tag="ACHKIR_002842"
     CDS             complement(6251..7066)
                     /gene="djlA"
                     /locus_tag="ACHKIR_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HFO9990552.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKLQKGFK"
     gene            7321..9675
                     /gene="lptD"
                     /locus_tag="ACHKIR_002843"
     CDS             7321..9675
                     /gene="lptD"
                     /locus_tag="ACHKIR_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HFO9990553.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            9728..11014
                     /gene="surA"
                     /locus_tag="ACHKIR_002844"
     CDS             9728..11014
                     /gene="surA"
                     /locus_tag="ACHKIR_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0042277 - peptide binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA];
                     GO_process: GO:0050821 - protein stabilization [Evidence
                     IEA];
                     GO_process: GO:0060274 - maintenance of stationary phase
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HFO9990554.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            11014..12003
                     /gene="pdxA"
                     /locus_tag="ACHKIR_002845"
     CDS             11014..12003
                     /gene="pdxA"
                     /locus_tag="ACHKIR_002845"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HFO9990555.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTAGTLTLLPVALRESVTAGQLAVENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDKLRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGKADVGSFITALNLAIKMIVNTQ"
     gene            12000..12821
                     /gene="rsmA"
                     /locus_tag="ACHKIR_002846"
     CDS             12000..12821
                     /gene="rsmA"
                     /locus_tag="ACHKIR_002846"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFO9990556.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDKLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            12824..13201
                     /gene="apaG"
                     /locus_tag="ACHKIR_002847"
     CDS             12824..13201
                     /gene="apaG"
                     /locus_tag="ACHKIR_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HFO9990557.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            13208..14050
                     /gene="apaH"
                     /locus_tag="ACHKIR_002848"
     CDS             13208..14050
                     /gene="apaH"
                     /locus_tag="ACHKIR_002848"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HFO9990558.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGTLTCLRWEDKHYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(14128..14607)
                     /gene="folA"
                     /locus_tag="ACHKIR_002849"
     CDS             complement(14128..14607)
                     /gene="folA"
                     /locus_tag="ACHKIR_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HFO9990559.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(14799..16661)
                     /gene="kefC"
                     /locus_tag="ACHKIR_002850"
     CDS             complement(14799..16661)
                     /gene="kefC"
                     /locus_tag="ACHKIR_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HFO9990560.1"
                     /translation="MDSHTLIQALIYLGSAALIVPIAVRLGLGAVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSSEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(16654..17184)
                     /gene="kefF"
                     /locus_tag="ACHKIR_002851"
     CDS             complement(16654..17184)
                     /gene="kefF"
                     /locus_tag="ACHKIR_002851"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HFO9990561.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVFSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(17292..18623)
                     /gene="yaaU"
                     /locus_tag="ACHKIR_002852"
     CDS             complement(17292..18623)
                     /gene="yaaU"
                     /locus_tag="ACHKIR_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9990562.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMF
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGLLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMG"
     gene            complement(18682..18969)
                     /gene="fixX"
                     /locus_tag="ACHKIR_002853"
     CDS             complement(18682..18969)
                     /gene="fixX"
                     /locus_tag="ACHKIR_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HFO9990563.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(18966..20252)
                     /gene="fixC"
                     /locus_tag="ACHKIR_002854"
     CDS             complement(18966..20252)
                     /gene="fixC"
                     /locus_tag="ACHKIR_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFO9990564.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(20303..21244)
                     /gene="fixB"
                     /locus_tag="ACHKIR_002855"
     CDS             complement(20303..21244)
                     /gene="fixB"
                     /locus_tag="ACHKIR_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFO9990565.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINTFVLNDADGAQA
                     IQLGANHVWKLSGKPDERMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LNAAVSNDASAVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDVAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(21259..22029)
                     /gene="fixA"
                     /locus_tag="ACHKIR_002856"
     CDS             complement(21259..22029)
                     /gene="fixA"
                     /locus_tag="ACHKIR_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HFO9990566.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     VLAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAEAA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            22502..24016
                     /gene="caiT"
                     /locus_tag="ACHKIR_002857"
     CDS             22502..24016
                     /gene="caiT"
                     /locus_tag="ACHKIR_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiporter"
                     /protein_id="HFO9990567.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVLGLTASTWILWTVLGSNTLLLIDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            24047..25189
                     /gene="caiA"
                     /locus_tag="ACHKIR_002858"
     CDS             24047..25189
                     /gene="caiA"
                     /locus_tag="ACHKIR_002858"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HFO9990568.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            25317..26534
                     /gene="caiB"
                     /locus_tag="ACHKIR_002859"
     CDS             25317..26534
                     /gene="caiB"
                     /locus_tag="ACHKIR_002859"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HFO9990569.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMTKGKDPYYAGCGLYKCADGYIVMELVGITQIAECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            26607..28160
                     /gene="caiC"
                     /locus_tag="ACHKIR_002860"
     CDS             26607..28160
                     /gene="caiC"
                     /locus_tag="ACHKIR_002860"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HFO9990570.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSSGVVNRYSYLELN
                     QEINRTANLFYVLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREEST
                     WILQNSQACLLVTSAQFYPMYQQIQQEDASQLRHICLTDMVLPADDGVSSFTQRKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATITECIPMMIRTL
                     MVQPPSANDRQHCLREVMFYLNLSEQEKDAFCERFSVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGELGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFCFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            28269..29054
                     /gene="caiD"
                     /locus_tag="ACHKIR_002861"
     CDS             28269..29054
                     /gene="caiD"
                     /locus_tag="ACHKIR_002861"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HFO9990571.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEAQRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            29060..29650
                     /gene="caiE"
                     /locus_tag="ACHKIR_002862"
     CDS             29060..29650
                     /gene="caiE"
                     /locus_tag="ACHKIR_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HFO9990572.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAIIGEESIVAAMSFVKAGFRGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYK
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(29769..30164)
                     /gene="caiF"
                     /locus_tag="ACHKIR_002863"
     CDS             complement(29769..30164)
                     /gene="caiF"
                     /locus_tag="ACHKIR_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HFO9990573.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(30425..33646)
                     /gene="carB"
                     /locus_tag="ACHKIR_002864"
     CDS             complement(30425..33646)
                     /gene="carB"
                     /locus_tag="ACHKIR_002864"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFO9990574.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHSMEEALAVAA
                     EVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNADFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     ERLKLKQPANATVTTIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(33664..34812)
                     /gene="carA"
                     /locus_tag="ACHKIR_002865"
     CDS             complement(33664..34812)
                     /gene="carA"
                     /locus_tag="ACHKIR_002865"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFO9990575.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
BASE COUNT         8343 a         9459 c         9140 g         7972 t
ORIGIN      
        1 caggctataa tcaagcctgg ttttttattg gattttcagc gtggcgcagg caggttttat
       61 cttaacccga cactggcggg ataccccgca aggaaccgaa gtttcctttt ggttagcgac
      121 ggacaacggg ccgttgcagg ttacgcttgc gccgcaagag tccgtggcgt ttattcccgc
      181 cgatcaggtt ccccgcgccc agcatatttt gcagggtgag caaggctttc gcctgacgcc
      241 gctggcgtta aaggattttc atcgccagcc ggtgtatggc ctttactgtc gcgcccatcg
      301 ccagttgatg aattacgaaa agcgcctgcg tgaaggtggc gttaccgtct acgaggccga
      361 tgtgcgcccg ccagaacgct atctgatgga gcggtttatc acctcaccgg tatgggtcga
      421 gggtgatata cgcaatggcg ctatcgttaa tgcccgtctg aaaccgcatc ccgactaccg
      481 tccgccgctc aagtgggttt ctattgatat tgaaaccacc cgccacggtg agctgtactg
      541 catcggcctg gaaggttgcg ggcagcgcat cgtttatatg ctggggccag agaatggcga
      601 cgcctccgcg ctcgattttg aactggagta cgtcgccagc cgcccgcagc tactggaaaa
      661 actcaacgcc tggtttgcga ctcacgatcc cgatgtgatc atcggctgga acgtggtgca
      721 gttcgatctg cgaatgctgc aaaaacatgc cgagcgttac cgtattccgc tgcggctggg
      781 gcgcgataac agcgagctgg agtggcgcga gcacggcttt aaaaacggtg tgttttttgc
      841 tcaggttaaa ggtcggctaa ttatcgacgg tatcgaggca ctgaaatccg cgttctggaa
      901 tttctcttca ttctcgctgg aaaccgtcgc tcaggagtta ttaggcgaag gaaaatctat
      961 cgataacccg tgggatcgaa tggacgaaat tgaccgccgt ttcgccgaag ataagcctgc
     1021 gctggcaact tataacctga aagattgcga gctggtgacg cagatcttcc acaaaactga
     1081 aatcatgccg tttttgctcg aacgggcgac ggtgaacggc ctgccggtag atcgacacgg
     1141 cggttcggtg gcagcgtttg gtcatctcta ttttccccgt atgcatcgcg ctggttatgt
     1201 cgcgcctaat ctcggcgaag tgccgccgca cgccagccct ggcggctacg tgatggattc
     1261 gcgacctggg ctttatgatt cggtgctggt gctggactac aaaagcctgt acccgtcgat
     1321 catccgcacc tttctgattg atcccgtcgg gctggtggaa ggcatggcgc agcctgatcc
     1381 agagcacagc accgaaggct ttctcgatgc ctggttctcg cgagaaaaac attgcctgcc
     1441 ggagattgtg actaacatct ggcacgggcg cgatgaagcc aaacgtcagg gtaataagcc
     1501 gctttcgcag gcgctgaaga tcattatgaa tgccttttat ggcgtgcttg gcaccaccgc
     1561 ctgccgcttc ttcgatccgc gcctggcgtc gtcgatcact atgcgtggtc atcagatcat
     1621 gcggcaaacc aaagcgttga ttgaagcgca gggctacgac gtgatctacg gcgataccga
     1681 ctcaacgttt gtctggttga aaggcgcaca ttcggaagaa gaagcggcga aaattggtcg
     1741 tgcactggtg cagcacgtta acgcctggtg ggcagaaacg ctgcaaaaac aacggctaac
     1801 cagcgcactg gaactggagt atgaaaccca tttctgccgt tttctgatgc caaccattcg
     1861 cggagccgat accggcagca aaaagcgcta cgccggactg attcaggagg gcgacaaaca
     1921 gcggatggta tttaaagggc tggaaaccgt gcgcaccgac tggacgccgc tggcccagca
     1981 gtttcagcag gagctgtacc tgcgtatctt ccgcaacgag ccatatcagg aatacgtgcg
     2041 cgaaaccatc gacaagctga tggcgggcga actggatgcg cggctggttt accgtaaacg
     2101 ccttcgccgt ccgctgagcg aatatcagcg taatgttccg cctcatgtac gcgccgctcg
     2161 ccttgccgat gaagaaaacc aaaagcgcgg tcgccccttg caatatcaga accgcggctc
     2221 cattaagtac gtatggacca ccaacggccc ggagccgctg gactaccaac gttcaccgct
     2281 ggattacgaa cactatctga cccgccagct acaacccgtg gcggagggaa tactcccttt
     2341 tattgaggat aattttgcta cacttatgac cgggcaactt gggctatttt gagcaaaaaa
     2401 agagttcgcc agataccatt ttgatgcgtg acgaatgctt tgccatccag taccatagcg
     2461 ccctttccat tcctggacct gaataacacc actacctcat aagcacggta gcgggtggtt
     2521 attgcctgca attaaagata tagagccgaa cacatatgcc ttttacactt ggtcaacgct
     2581 ggatcagcga tacagaaagc gaattgggac ttggaaccgt tgtcgcggtg gatgcgcgaa
     2641 ctgtcacttt acttttccca tctactggtg aaaaccgtct gtacgcacgc agtgattccc
     2701 ccgtgacccg cgtgatgttc aaccctggtg atactattac cagccatgac ggctggcaga
     2761 tgcaagtcga agaagtaaaa gaagaaaatg gcttgctgac ctatatcggt actcgcctgg
     2821 atactgaaga gtccggcgta gccctgcgtg aagttttcct tgatagcaaa ctggtgttca
     2881 gcaaaccgca ggaccgtctg tttgccgggc agattgaccg tatggaccgc tttgcgctgc
     2941 gttatcgcgc gcgtaagtat tccagcgaac agttccgtat gccgtacagc ggcctgcgcg
     3001 gtcagcgtac cagcctgatc ccgcaccagc tcaacatcgc tcatgatgtt ggccgccgcc
     3061 acgcgccgcg cgtcctgctg gctgacgaag tgggtttagg gaaaaccatt gaagccggga
     3121 tgatcctgca tcagcaactg ctctctggcg ctgctgaacg tgtgctgatt atcgtcccgg
     3181 aaaccttaca gcatcagtgg ctggtagaaa tgctgcgccg tttcaacctg cgctttgctc
     3241 tgtttgatga tgagcgttat gccgaagctc agcacgatgc ttacaacccg ttcgataccg
     3301 aacagctggt gatttgctcg ctggattttg ctcgtcgtag caaacagcgt ctggaacatc
     3361 tctgtgaagc cgaatgggat ctgctggtgg tcgatgaagc ccatcacctg gtgtggagcg
     3421 aagatgcgcc gagccgcgaa tatcaggcca ttgaacaact ggcagagcac gtgccgggcg
     3481 ttctgctgct gaccgcaacc ccggaacagc tggggatgga aagccacttc gcccgtctgc
     3541 gtctgctgga cccgaaccgt ttccacgatt ttgcgcaatt cgttgaagag cagaaaaatt
     3601 atcgtccggt tgcggacgcc gttgccatgc tgctggcagg taacaaactg agcaatgatg
     3661 aactgaacat gctcggtgag atgatcggcg agcaggatat cgagccgctg ttgcaagcag
     3721 caaacagcga cagcgaagat gcccagagcg cccgtcagga actggtttcg atgctgatgg
     3781 atcgccacgg caccagccgc gtgctgttcc gtaacacgcg taacggtgtg aaaggcttcc
     3841 cgaaacgcga gctgcatact attaagctgc cactgccgac gcagtatcag accgctatta
     3901 aagtctccgg cattatgggc gcacgtaaaa gtgcggaaga tcgcgctcgc gatatgctct
     3961 acccggagcg tatttatcag gaatttgaag gcgataacgc cacctggtgg aacttcgatc
     4021 cgcgcgttga atggctgatg ggctacctga ccagccatcg ctctcagaaa gtgctggtga
     4081 tctgcgccaa agctgccact gcgctgcaac tggagcaagt actgcgcgaa cgtgaaggta
     4141 tccgcgctgc tgtgttccac gaaggcatgt cgattatcga acgtgaccgc gctgccgcct
     4201 ggtttgctga agaagacacc ggtgcgcagg tactgctgtg ctcggaaatc ggttctgaag
     4261 gacgtaactt ccagttcgcc agccacatgg tgatgtttga cctgccattc aacccggatc
     4321 tgctggagca gcgtattggt cgtctggatc gtatcggtca ggcgcacgat attcagatcc
     4381 atgtgcctta tctggaaaaa actgctcagt cggtgctggt gcgctggtat cacgaaggtc
     4441 tggatgcatt cgagcacacc tgcccgaccg gacgcactat ttacgatagt gtatacaacg
     4501 atctgattaa ctatctggct tcaccggatc aaaccgaagg ctttgacgat ctgatcaaaa
     4561 actgccgcga gcaacatgaa gcgctgaaag cacagctgga acaaggtcgt gaccgcctgc
     4621 tggaaatcca ttccaacggt ggcgaaaaag cccaggcact ggcagaaagc attgaagagc
     4681 aggatgacga taccaacctg atcgccttcg ccatgaacct gttcgatatt atcggtatca
     4741 atcaggacga tcgcggcgac aacatgatcg tgctgacgcc gtccgatcat atgctggtgc
     4801 cggacttccc tggcctgtcg gaagatggca tcaccatcac cttcgaccgt gaagtggcgc
     4861 tggcgcgtga agatgcacag tttattacct gggagcatcc gctgatccgc aacggtctgg
     4921 atctgatcct ttctggcgat accggtagca gcacgatttc actgttgaaa aacaaagcgt
     4981 tgccggtagg tacgctgttg gtggaactga tttatgtggt tgaagcccag gctccgaagc
     5041 agttgcagct caaccgcttc ctgccaccga cgccggtacg tatgctgctg gataaaaacg
     5101 gcaacaacct ggcggcgcag gtagagtttg aaacctttaa ccgccagctt aacgcggtta
     5161 accgtcacac cggcagcaag ctggttaacg ccgtgcagca ggatgttcac gctatccttc
     5221 agctggggga agcgcagatc gagaaatctg cccgtgcatt gattgatgcc gcacgtaacg
     5281 aagccgacga aaaactgtct gccgagctgt ctcgtctgga agcgctgcgt gcagttaacc
     5341 cgaacattcg tgacgacgaa ctgaccgcca ttgagagcaa ccgtcagcag gtaatggaaa
     5401 gcctggatca ggcaggctgg cgtctggatg ccctgcgttt gatcgttgtg acgcatcagt
     5461 aacggagccg aagatgggga tggaaaacta caatccgccg caggaaccct ggctggttgt
     5521 cctgtatcag gatgaccata ttatggtggt caacaagccg agcggtttgt tgtctgtgcc
     5581 gggtcgtctg gaagagcaca aagacagcgt gatgacgcgc attcagcgtg attatccgca
     5641 ggcagaatcg gtgcatcgtc tggatatggc taccagcggc gtgattgtag tggcgctgac
     5701 caaagccgcg gagcgggagt taaaacgcca gttccgcgag cgcgagccta aaaaacagta
     5761 tgtggcccgc gtctgggggc atccatcccc tgcggaaggt ctggtggatc tgccgctgat
     5821 ttgcgactgg ccaaaccgcc cgaaacagaa agtctgttac gaaacaggta aacctgcgca
     5881 gacggagtat gaagtggtgg aatatgcggc ggataacacg gcaagagtgg tgttaaaacc
     5941 gattaccggg cgttcgcatc agctgcgtgt gcatatgctg gcgctgggtc atccgattct
     6001 cggcgatcgt ttttatgcat caccagaagc gagagcaatg gcaccacgtt tgttgctaca
     6061 tgcagagatg ctgacgatta cccatccggc gtatggcaat agtatgacgt ttaaagcacc
     6121 agcggatttt taatagtcac gctggcgaag ttcggtaggc cggagaaggc gttcacgccg
     6181 catccggcaa ttttgcgccg attgcctgat gcgacgcggg ccgcgtctta tcaggcctac
     6241 acgttgaaac ttatttaaac cctttctgca gcttaatcaa ttcataggcc tgctgaattt
     6301 cctgcgcttt ttgcttcgcc atctccatca tctcaggcgg caaacctttc gccaccagct
     6361 tatcgggatg gtgttcactc atcagcttac ggtaggcacg tttgatggtg gtcgcatcat
     6421 ccgtcggctt cacgcccagc acattacagg catcttccag cgttgggcca cgctgcgctt
     6481 gctgccagtt accaccgcca gattgctgct gataaccgcc gccaaactgt gcaccgccct
     6541 gcatcatgcg caaaaactgg tcaaactggg cgcgggagat ccctaattct tcagcaatga
     6601 catacagcac agcccgttca ttcgggtgca gtgaaccatc agcaaacgcc gcctgaatct
     6661 ggatctccag aaacatacga attaagtcaa aacgaccaaa gcagacactg cgaaactggc
     6721 gcatcttttc gcgcagcggg taattgtctg attttcccac ccggaacgcg ttttgcgccg
     6781 cagtacggga agcgccatga aggttcattc ggtccatcaa ctggctggcg atatgaatat
     6841 cagcctccgt gacgcgacct ttggatttgg ttaaatgccc catcacttca aaagtggtgg
     6901 caaaaaacag cgcctgacgc tcacgctggt tggcgaacca cgccatttta cggctacggg
     6961 ctttatcaaa catatggcca attaacaggc ctaacactac gccccaaaag ccgccgccca
     7021 tcagtaaggc cacggccacg ccaatgattt ttccccaata ctgcatatat tccccaaatc
     7081 gacacacgga tatcagggct atctcccaca atataaaggt gcttttaccg ttttccggct
     7141 gcggtcaatt gtgggacatc gcctataatt tgcattatca tacctgtcat tcaatgccgt
     7201 gcctaacacc actgacgcta ttcggacagg attaacacta gcgtagagat gacgagtacg
     7261 ttagtctctg accgtttgtc acgcgcaacg ttaccgatga tggaacaata aaatcaacgt
     7321 atgaaaaaac gtatccccac tctcctggcc accatgattg ccaccgccct ttatagtcaa
     7381 cagggactgg cagccgacct cgcctcacag tgcatgttgg gcgtgccaag ctatgaccgt
     7441 cctctggtac agggcgatac caatgactta cccgtgacta tcaatgctga ccacgcgaaa
     7501 ggggactacc cggatgacgc cgtgtttact ggcagcgtgg atatcatgca gggtaacagc
     7561 cgtctgcagg ccgacgaagt gcagctccat caaaaagagg cgccaggaca accagagccg
     7621 gtacgtaccg ttgatgcgct cggtaatgtc cattacgacg ataaccaggt gatcctcaaa
     7681 gggccgaaag gctgggcgaa tctgaacacc aaagatacca acgtctggga gggtgattac
     7741 cagatggtgg gtcgccaggg tcgcggtaaa gcggacctga tgaaacaacg tggcgaaaac
     7801 cgctatacca ttctggataa cggtagcttt acctcctgtc tgccgggttc tgacacctgg
     7861 agcgtggtag gcagcgaaat tattcatgac cgcgaagaac aagttgcgga gatctggaac
     7921 gcccgcttta aggtgggtcc ggtaccgatc ttttatagcc cctatttgca gttgccggtg
     7981 ggtgacaaac gtcgctctgg tttcttgatc ccgaacgcca agtacaccac caccaactac
     8041 tttgagttct acctgccata ttactggaac atcgcgccaa atatggatgc caccatcacg
     8101 ccgcattata tgcatcgtcg tggcaatatc atgtgggaga acgaattccg ctacctctcc
     8161 caggcgggcg ctggcttgat ggaactggac tatctgcctt cagataaagt ctatgaagat
     8221 gaacacccga acgatgacag ttcacgtcgt tggttattct actggaacca ctccggggtc
     8281 atggatcagg tgtggcgttt caacgtcgac tacaccaagg tcagcgatcc tagctacttc
     8341 aatgattttg ataacaagta cggttccagt actgacggct acgcaacgca aaaattcagc
     8401 gttggctatg cggtgcaaaa cttcaatgcc accgtttcaa ccaagcagtt ccaggtcttc
     8461 agcgagcaga acaccagtag ctactcggca gagccgcagt tagacgttaa ctactaccag
     8521 aatgatgttg gtccgtttga tacacgcatt tacggccagg cagtgcactt tgttaacacc
     8581 agagacgaca tgcctgaagc aacccgtgtt cacctggaac cgaccatcaa tttgccgctc
     8641 tctaataact ggggcagcat caataccgaa gcgaagttgc tggcaaccca ttatcagcaa
     8701 actaatcttg actggtataa ctccagaaac acaaccaagc tggacgaatc cgttaaccgc
     8761 gtaatgccgc aattcaaagt tgacggcaaa atggtctttg aacgcgatat ggaaatgctg
     8821 gcgccgggtt atacccaaac gctggaaccg cgcgcgcagt atttatacgt gccgtatcgc
     8881 gatcagagcg acatctataa ctacgactcg tctctgctgc aatctgacta ctctggcctg
     8941 ttccgggacc ggacttacgg cggtcttgac cgtattgcct ccgctaacca ggtgacgacc
     9001 ggtgtcacat ctcgcatata tgatgatgct gccgttgaac gttttaatat ttccgttggt
     9061 caaatctact atttcacgga gtctcgcact ggcgatgaca acataacatg ggagaatgac
     9121 gacaaaacgg gctcactggt gtgggcaggc gatacttact ggcgtatctc agagcgttgg
     9181 ggattgcgtg gcggaattca gtacgataca cgtctggata acgtagcgac cagtaactcc
     9241 agcattgaat accgtcggga tgaagaccgt ctggtacagc tgaattaccg ttacgccagc
     9301 ccggaatata ttcaggctac gctgcctaag tactattcca ctgctgagca atataagaat
     9361 ggtatttcgc aggtaggtgc cgtcgccagc tggccaattg ccgatcgttg gtcaattgtt
     9421 ggggcctact actacgacac caatgcgaac aagcaagccg actctatgtt aggtgtgcaa
     9481 tacagctcct gctgctatgc aattcgcgtc ggttacgagc ggaagctgaa cggttgggat
     9541 aacgataaac aacatgcggt atatgacaac gcaatcggct ttaacatcga acttcgcggc
     9601 ctgagctcca actacggtct gggtacgcaa gagatgctgc gttcgaacat tctgccgtat
     9661 caaaacactt tgtgatttgt tgatttacca cgtaatccgc agtgcggtta attgaaatgg
     9721 aaaaagtatg aagaactgga aaacgctgct tctcggtatc gccatgatcg cgaataccag
     9781 tttcgctgcc ccccaggtag tcgataaagt cgcagccgtc gtcaataacg gcgtcgtgct
     9841 ggaaagcgac gttgatggat taatgcaatc ggtaaaactg aacgctgctc aggcaaggca
     9901 gcagcttcct gatgacgcga cgctgcgcca ccaaatcatg gaacgtttga tcatggatca
     9961 aatcatcctg cagatggggc agaaaatggg agtgaaaatc tccgatgagc agctggatca
    10021 ggcgattgct aacatcgcga aacagaacaa catgacgctg gatcagatgc gcagccgtct
    10081 ggcttacgat ggtctgaact acaacaccta tcgtaaccag atccgcaaag agatgattat
    10141 ctctgaagtg cgtaacaacg aggtgcgtcg tcgcatcacc atcctgccgc aggaagtcga
    10201 atccctggcg cagcaggtgg gtaaccaaaa cgacgccagc actgagctga acctgagcca
    10261 catcctgatc ccgctgccgg aaaacccgac ctctgatcaa gtgaacgaag cggaaagcca
    10321 ggcgcgtgcc attgtcgatc aggcgcgtaa cggcgctgat ttcggtaagc tggcgattgc
    10381 tcattctgcc gaccagcagg cgctgaacgg cggccagatg ggctggggcc gtattcagga
    10441 gttgccgggg atcttcgccc aggcattaag caccgcgaag aaaggcgaca ttgttggccc
    10501 gattcgttcc ggcgttggct tccatattct gaaagttaac gacctgcgcg gcgaaagcaa
    10561 aaatatctcg gtgaccgaag ttcatgctcg ccatattctg ctgaaaccgt cgccgatcat
    10621 gactgacgaa caggcccgcg tgaaactgga acagattgct gccgatatca agagtggtaa
    10681 aacgactttt gctgccgcag cgaaagagtt ctctcaggat ccaggctctg ctaaccaggg
    10741 cggtgatctc ggttgggcta caccagatat tttcgatccg gccttccgtg acgccctgac
    10801 ccgcctgaac aaaggtcaaa tgagtgcacc ggttcactct tcattcggct ggcatttaat
    10861 cgaactgctg gatacccgta atgtcgataa aaccgacgct gcgcagaaag atcgtgcata
    10921 ccgcatgctg atgaaccgta agttctcgga agaagcagca agctggatgc aggaacaacg
    10981 tgccagcgcc tacgttaaaa tcctgagcaa ctaatggtta aaacccaacg tgttgtgatc
    11041 actcccggcg agcccgccgg gattggcccg gacttagttg tccagcttgc acagcgtgag
    11101 tggccggtcg aactggttgt ttgtgccgat gccactctcc ttaccgaccg ggcagcgatg
    11161 ctcggtttgc cgctcaccct ccgtccttat tcccccaact cccctgcaca accgcaaact
    11221 gcgggcacat taacgctact tcctgtcgcg ctacgtgaat ctgtcactgc ggggcagtta
    11281 gcggttgaaa atgggcatta cgtagtggag acgctggcgc gagcgtgtga tggctgtctg
    11341 aacggtgaat ttgctgcgct gatcacaggc cccgtgcata aaggcgtcat taacgacgca
    11401 ggcattccgt ttaccggtca taccgagttt ttcgaagagc gttcgcaggc gaaaaaagtg
    11461 gtgatgatgc tggcgaccga agaacttcgc gtggcgctgg caacgacgca tttaccgctg
    11521 cgcgatatcg cagatgctat cacccctgcg cttttgcacg aagtgattgc tattttgcat
    11581 cacgatttgc ggaccaaatt tggtattgcc gaaccgcgca ttctggtctg cgggctgaat
    11641 ccgcacgcgg gcgaaggcgg tcatatgggt acggaagaga tagacaccat tattccggtg
    11701 ctcgacaagc tgcgggcgca ggggatgaaa ctcaacgggc cgctgcctgc cgataccctg
    11761 tttcagccga aatatcttga taacgccgac gcggtgctgg cgatgtacca cgatcagggt
    11821 cttcccgtgc taaaatacca gggcttcggg cgcggtgtga acattacgct gggcctgccc
    11881 tttattcgca catcagtgga ccacggtacc gcgcttgaac tggcgggacg tggcaaagcc
    11941 gatgtcggca gttttattac ggcgcttaat ctcgccatca aaatgattgt taacacccaa
    12001 tgaataatcg agtccaccag ggccacttag cccgtaaacg cttcgggcaa aactttctca
    12061 acgatcagtt cgtgatcgac agcattgttt ctgccattaa cccgcagaag ggccaggcga
    12121 tggtcgaaat cggccccggt ctggcggcat tgaccgaacc ggtcggcgaa cgtctggata
    12181 agctcacggt catcgaactt gaccgcgatc tggcggcacg tctgcaaacg catccattct
    12241 taggcccgaa actgactatt tatcagcagg atgcgatgac ctttaacttt ggtgaactgg
    12301 ccgagaaaat gggtcagccg ctgcgtgttt tcggcaacct gccttataac atctccacgc
    12361 cgttgatgtt ccatctgttt agctatactg atgccattgc cgacatgcac tttatgttgc
    12421 aaaaagaggt ggtgaatcgt ctggttgcag gaccgaacag caaggcgtat ggtcgattaa
    12481 gcgtcatggc gcaatactat tgcaacgtga tcccggtgct ggaagtaccg ccgtcagcct
    12541 ttacaccacc acccaaagtg gattccgccg tcgtgcgcct ggttcctcat gcaacgatgc
    12601 ctcacccggt taaagatgtt cgcgtgttga gccgcatcac caccgaagcc tttaaccagc
    12661 gtcgtaaaac cattcgtaac agcctcggca acctgtttag cgtcgaggtg ttaacgggaa
    12721 tggggatcga cccggcgatg cgagcggaaa atatctctgt cgcgcaatat tgccagatgg
    12781 cgaactatct ggcggagaac gcgcctttgc aggagagtta acgatgatca attcgccccg
    12841 agtgtgtatt caggttcaaa gcgtctacat tgaggcacaa tcttcacctg ataatgaacg
    12901 ttacgttttt gcttataccg taaccatacg caatctgggg cgagcgccag tgcagttgtt
    12961 ggggcgttac tggctgatca ccaatggcaa tggccgtgaa accgaagtcc agggcgaagg
    13021 agtggttggc gtccagccac ttatcgcacc tggcgaagag taccagtaca ccagcggtgc
    13081 aatcattgaa accccgctgg gcaccatgca gggtcactac gaaatgatcg atgaaaatgg
    13141 cgtccctttc agcatcgaca ttcccgtatt ccgactcgcc gttcccacac tcattcatta
    13201 aaagaatatg gcgacatacc ttattggcga cgttcatggt tgttacgatg aactgatcgc
    13261 attgctgcat aaagtagaat ttacccctgg gaaagatacc ctctggctga cgggcgatct
    13321 ggtcgcgcgc ggcccgggtt cgctggatgt tctgcgctat gtgaaatcct taggcgacag
    13381 cgtacgtctg gtgctgggca atcacgatct gcatctgctg gcggtatttg ccgggatcag
    13441 ccgcaataaa ccgaaagatc gcctgacacc gctgctggaa gcgccggatg ccgacgagct
    13501 gcttaactgg ctgcgtcgcc agcctctgct gcaaatcgac gaagagaaaa agctggtgat
    13561 ggcccacgcc gggatcacgc cacagtggga tctacagacc gccaaagagt gcgcgcgcga
    13621 tgtagaagcg gtgctgtcga gcgactccta ccccttcttc cttgatgcca tgtacggcga
    13681 tatgccaaat aactggtcac cggaattgcg ggggctggga agactgcggt ttatcaccaa
    13741 cgcctttacc cgtatgcgtt tttgcttccc gaacggtcaa ctggatatgt acagcaaaga
    13801 atcgccggaa gaggcccctg ccccactgaa accgtggttt gcgattcctg gccctgtcgc
    13861 tgaagagtac agcatcgcct ttggtcactg ggcatcgctg gaaggcaaag gtacgccgga
    13921 aggtatttac gcgctggata ctggctgctg ctggggtggt acattaacct gcctgcgctg
    13981 ggaagataaa cactattttg tccagccgtc gaaccggcat aaggatttgg gcgaagcggc
    14041 ggcgtcttaa acacagcctg atacaggaag gccggataag acgcgaccgg cgtcgcatcc
    14101 ggcactagcc gtaaattcta tacaaactta ccgccgctcc agaatctcaa agcaatagct
    14161 gtgagagttc tgcgcatcag cgtcgtggaa ttcgctgaat accgattccc agtcatccgg
    14221 ctcgtaatcc gggaaatggg tgtcgccttc cacttctgcg tcgatatgcg tcagatacag
    14281 tttttgcgct ttgggcagga actgctcata aacgcgaccg ccgccaatca ccatgatttc
    14341 tggtacgtca ccacacgccg caatggcttc atccaccgac ttcacccacg ttacgcgatc
    14401 gtccgtaccc ggttgactgc tgaggataat atttttgcgt cctggcaacg gacgaccgat
    14461 ggattcccag gtatggcggc ccataatcac gggtttattt aaagtgttgc gtttaaacca
    14521 ggcgagatcg gcaggcaggt tccacggcat ggcgttttcc atgccgataa cacgatctac
    14581 cgctaacgcc gcaatcagac tgatcattga gatttcccga taaaaaaaat tgtcgccact
    14641 atacgtaaag cgtaaaccgt cgtcgactgg tgcgaggatg atgttgagga aaattttata
    14701 ttctgctggc gagtccacgc tctcccactg gactcgccgc attacaatga aataaaaaca
    14761 attagttagc tgtaaagtgt gatttacgtc actctttatt aggatgaggg tttcgtttcc
    14821 ggttcatccg ccatgttgcc ggtatgttta ccttcttcgg ttccctgcca gccatgtcgt
    14881 tgaattaatg acagatgttc gcggtcctcg gtaatgatct cacttaacat cgcgctggtg
    14941 cgtttataga ccgccgcgcg ggctttggtg tcgttctcaa ccattgccat ctcttccacc
    15001 atctgaatat taaagcggcg gaacacatcg gcgcgttctc gcgcttcata cggccccaga
    15061 cctaaacttt ccagtgccag acgcccggtt ttcagcgcac cttcaaaggt ttcacgctcc
    15121 ggcttttcaa cgcctgcctg acgcaaacga atgtagtggt cgacatcgcg ggcgcgggca
    15181 ataatctgca aatgcgggaa atgttctttc accatctccg tcagttgcag gttggtttgc
    15241 ggatcgtcga tagcgttaat cagcacttcc gctttcgccg ctccggcaga ttccagtaaa
    15301 tccatccgcg tggcatcgcc ataaaacact ttcataccaa atttacgcaa ggtttcgata
    15361 tggtccggat cgtgatcgag caccaccatt ttcaccccgc tggagagcag taaacgtccg
    15421 gtgatctgcc caaaacgacc gaatccggcg atgattaccc gcggctgttc ttcgtcgatc
    15481 tcatcggctt cacgcgcctc ttcactagaa gattgctcaa ggcgattgag gatcaccagc
    15541 agaatcggcg ttgccgccat cgacagcgcc accgccaggg tcagcgattt cgcccactcc
    15601 ggctccagca cattcgccat ctgcgccgca ccaaatacta caaaggcaaa ctcactaccc
    15661 tgccctaata gcaccgcaaa ccagcgacgc tgtttatttg gcacctgcaa cggccgggca
    15721 atcagccaca gcatggcgat tttgatgatg aggaaaccga gcagcaaaat gacaatgcgc
    15781 aacggatttt caagcagcgt gccaaagtct atcgacatgc caacgccgat gaaaaacagc
    15841 cccagcagca aacctttaaa tggttcgata tcgctttcca gcgcatgacg gtattccgag
    15901 cttgccagca gtacgcccgc cagaaacgcg cccatcgcca tcgacaagcc gacctcttcc
    15961 agcagcaaac caaagccaaa cacgaggaat aacgccacgg cactaaacac ttcccgcaag
    16021 ccagagcggg caacaaaacg cagtgccgga cgcgtgacat agcgccccag caataccacc
    16081 agcaccagcg cacccgccac ttttaacgcc gagagagcaa atgcgcccat cgtcgtcgag
    16141 gcactgctcg ccgccagtag cgggatcatc gccaccagcg ggatcgccgc gatatcctgg
    16201 aacagcagca ccgcaaaggc actgcgaccc atttgcgtca ccatcagatt gcgttcattc
    16261 atcgcctgca tggcaatcgc cgtagaggag agcgccagcg tcatgccgat caattccgcg
    16321 acctgccagc gcagcccaag caacatacag aacagcccca gcaggccgcc gcaaatcacc
    16381 atctgcaatg cgccgccgcc gaacaccgcc gcacgcagct tccacagcct ttgtggatcg
    16441 agttcgaggc cgataataaa cagcatcagc accacgccaa tctcggcaaa gtgcagaata
    16501 gattcggcat cggtcaccag tcgcagcccc cacgggccaa taatgcagcc ggcgatcagg
    16561 tagccaagta ccgctcccag accgagacgt accgcaatgg gaacaatcag cgccgccgaa
    16621 ccgagataaa tcagcgcctg aataagcgta tggctatcca tgatgtgcct cctgccattc
    16681 cagcagacgt tgcttatagt gccgcgcctg cccttcgagg gtttcgtcgt cacaaataaa
    16741 ggtgcagtgc atggcaaacg gtggcagcca gttcagcccg cagtagattg ccgtcgcctg
    16801 tagcggctgt gacagcacat caaagcccgg atgcgcacca atctcaaaat ggctttcccc
    16861 accgccggtc gtcaccgccc acagtaaatg tttgccatgc agcgccgtgc cgccgtgacc
    16921 gtaagcccag ccgtgcgaga agactttatc gatccaaagt ttaagaagcg gaggaatgct
    16981 gtaccactgc atcgggtgct gccagacgat cagatcggcg cgagacagcg cctcctgctc
    17041 ggcggcaata tcgatattga agtcaggata gagttgataa agagagcgaa tttcgacgcc
    17101 ttccagcgtc cttgcctgtt caagcatccg tttattcgca tgggaatgat gcggatacgg
    17161 atgcgcataa attataagaa tcataggtag cccgcgtgat tacgcctttg ttttcccaca
    17221 aagtgtagtc atatattcac gcggcttata gtcaataata ttgattcaga aaagagagaa
    17281 atctgaacaa attaccccat tctctgcccg cggatcgtca tattgctggt ctgcgccagt
    17341 gacatccctc gagtctccgg ggcaaacgct acggaaatca acaagccaaa cagcgagata
    17401 cccgccccca ttagcatcgt gttactgata ccgtaattat tgataaagat cggcagtgcc
    17461 caggtcgaaa caatagtgcc aatacggctt aaggacataa tcacgcccac ggcagaggcg
    17521 cggatatccg tcgggaagag ttcattagga tagagccact gcaaattacc cggcccgcca
    17581 gagaaaaagg catacaccgc aaatgccatc actaccagcc agatccccat atccgggatc
    17641 agccccaaaa ccgccagcgc cagcgtcatc atggcaaagc tgccaatcaa caatggacgc
    17701 cgtccggcag tgtttagcca cagcatcggt ggaatacagc cgagcataaa gaacaggcta
    17761 atcaccacgt tccccagtgc cgcgtttttg ccaacaccca atcccaacaa accaacgatt
    17821 tgcgggccaa aggtgtagat ggcgaacatc gggatcacct ggcaggtcca gatggcggca
    17881 acaaacagaa caaaaggaaa atggcggcga ttaaacagat cgcgaaaacg ggtttgctgc
    17941 ggctgctctt catcgaaagc caccggttcg ccaaacagtt tgatcatcat ctcttcgcac
    18001 tcttttactc gccctttgcg taacaaccag cggggagatt caggcagttc gaatcgacca
    18061 atcaaaatca acaaacaggg gatcgctgcg ctacccagca tccagcgcca accaccttcc
    18121 acatcataaa gccagtaacc gaccagatcg gcgcaggtcg cgccgacata ccacatcgcg
    18181 gcaataaagc tgatggaaaa cgcccgctga cgggtactgg agaactcagt gatcatcgaa
    18241 gtggcgatgg gataatctgc accgatgaca atgccgatga gtacccgcat cactaacagt
    18301 tcgacggggg atgagacaaa catcgtcgcc accgatatca cgccgatggc gatgatatca
    18361 atgaggaaca ttttgcgccg tccgacttta tcggagatat agccaaacag cgatgtgcca
    18421 acgaacagcc cggcgagcgt tcccgcgccc agcaagccaa tccagtcagc gtccagtttc
    18481 agcgccggag tcagttgctc cagcgccacg ccaatcatta ccagtacata accatccaga
    18541 aacggaccac cgcttcccca cagcaaaatg cggcggtgaa tagaggagaa tttaagatca
    18601 tcaaagtttc tggacggttg catgggttat tcctgttttt ttgcaatgag agtcagtacg
    18661 gcattacgta tcaaaccaac atcagccgta acggaactcc acaccaaagg tgccgcgcgg
    18721 gtattcccac tgttccagcg ccgaccccag ccccagaatg cgacaggtgc cgcactccag
    18781 acatccggcg taatcgaagc gcacactgcc gtcatcctgc ttcttgtaca gacctgcggg
    18841 gcacgctttc accagcagct ccagggcctg tttatcggca tcggccttca caacgatgtg
    18901 cggatgctct tcatcgacat tgaatttatt gacgcccagt ttgacgtcca cattgacggg
    18961 agaagtcata aaacggtcac tcctttcatg ccatccttga tcagattgat gaagcccact
    19021 ttcttgccgt ggcggaggat tttcttgcgc atcagttccg gcgcactgcc gtcaatggtg
    19081 aacaggtcac gcgccacgcc caccgccagt tccgggtagc cgctaaacat gcgtgggtta
    19141 tcaaggaacg ccggtagttt ctggtacatg cgcatatcgc gcagcgggcc actctcaaga
    19201 tgctgacgat attccgcgag tttttgctta ctgaaatcgt cgcttttcat cgctgaaagc
    19261 acagtttttg ctgctgcttc cccggcggca atcgccagat ccataccgcg aatggtaaaa
    19321 ccgaggttca tacacattcc ggcagcatca ccggcaatca atacgccgtc accaaccaac
    19381 tccggcagca tgttgatacc tgcttccggc actacgtgag cggaatattc caccagcttg
    19441 ccgcccgcga tcagcggtgc aacggccgga tgttgtttga aatcttccag catttgcggc
    19501 accgattttt ttgcgtcatg cagatgatgc aaaccacaaa ccagccccag cgacagggtg
    19561 ttttcattgg tataaagaaa gccgccgccc atcaggccat cagtggggga acccgcaaac
    19621 aggcaagccg caccctgatt accctgcaac tgaaaacggt cttcgataac cgacttcggt
    19681 aattcgatca gttccttcac gccaaccgcc acatccgtcg gtttaacgcg ttttgccatc
    19741 cccagctttt cggcaaggat ggagttcacc ccgtcagcaa ggatcaccgt tttcgcttca
    19801 attacatcgc catcggcttc tacgccgaca actttgccat cgcgctgtac gaggttatcg
    19861 acacgaatcc cggtaattaa ctgcgcaccc gcttcttcgg cctgctccat cagccaggca
    19921 tcaaatttac tgcgcaaaac ggagtaagaa cgctgggatg gtgaggtttc gtcaccgttg
    19981 cagtagtcca tagtcatcgc agacttttcc gtcataaacg cgagtttttc atgggtgatc
    20041 aggcgttcta cgggggcgga gtcagcgaaa ccaggaataa tgtgttccag actgtgggca
    20101 tagagacgcc cgccggtgac gttcttggca ccagcggaat tgcctcgctc gataactaac
    20161 acttgcgccc cttcgcgggc gagcaccagt gcggcaaccg aaccggcaag ccctgcaccg
    20221 acgatgatgg cgtcaaagat atcttcggac ataagagctc cctgtcagcg gcagggccaa
    20281 aatgcagccc tgccagagtg gatcaacgcg ctaaagctgc ggtcagtgcc ggaaggatct
    20341 tcacggcgtc gccaacaatg ccgtaatccg cgtactggaa gatcggcgca tttttatctt
    20401 tgttgatggc gaaaatggtt tgcgacgcat tagcgccaac catgtgctgg atctgcccgg
    20461 agatccccac cgccaggtac agttcaggtt tcagcatcag gttggagata ccgacatagc
    20521 gttcgtgctc catccatttt tcgttttccg ccaccggacg agaacaggcc aactccgcac
    20581 ctatcgcctt gcaaagctgt tctgccagcg caatgttctc tttgctgcca ataccgcgac
    20641 caacgctgac caccaggcgg gctttgtcga gatcgacgct gttgctctga cgcgcctggg
    20701 ttgccgtgcg ggtaatcgcc acagccggag cctgccactc cacggtgtgc gtttcgccag
    20761 tgcgtgacgc gtctggctga gccacatcga acgtgccgct gctgatggtc agaaccgcat
    20821 acggcgtggc aatgcgttct tcgccaatcg ccagaccgcc gtaaaccatg tgtttcactg
    20881 tcgctttacc gtcctgcacg ctaacagcgc tggcatcgtt agacaccgcc gcgttaaggc
    20941 ggtaacccag tttcgccgcc agtaatttgc cgcgacgggt gtttggcaac agcaccagac
    21001 cgtctgcgcc gtgctggcga atagtgtcag ccatgacacc ggcgtaatct tcgatcatcc
    21061 gctcgtccgg tttgccgctt aatttccaga catgattagc gccgagctgg attgcctgtg
    21121 cgccgtcggc atcattgagg acgaacgtgt tgatttgatt agctaaagcc tgcgcaccgt
    21181 tcatcagttc cggcagacga gaaggggtat cgctgaatac ccagacttga gaaaacgtgt
    21241 tcatagcatc ccctgtaatt aaatgacttt gcgaagattt tcagcaaatg cggcgatctg
    21301 ttcttcgccg tcgccttcaa tcacgatgcg ctgacgctcg cgctgtttcg gcgcggcaac
    21361 ctgttgttct gaccaggctg cctctgcgtt aaaaccgata tccgccgccg accatacctg
    21421 gactggcttt ttcgccgcgc cgagaatggc tttcatcgaa ggaatttgtg gggagttgat
    21481 atcagtggaa acagcaacaa ccgcaggcag cggaatgctt aaggtttctg tttcatcttc
    21541 cagttcgcgc tcaacggtga gggtatctgc agtcagggag ataattttgc tgacgccgtt
    21601 aactgccgga atattaagga tttcgcctac cagcaaacca acctgctggg cataaaggtc
    21661 ggaagaacca tcgccacaga ggatcaaatc aaagcctgct ttctgagcgg ctgcagccag
    21721 tacgctcgcc gtttgttgcg gcagtgcctg ctcgaactgg tcatcaatca ccacaatcag
    21781 ttcatccgga ccgcgcgata gcacatcttt acgccctttg gcgttggtca gggctttacc
    21841 gcccacactt aaggccgtca cctgcgcctc tgctgcctgt tgctttagct ggcaagccgc
    21901 ttcaatagcg ttgagatcgt attggcttat tttggcatcg gctttgctga agtctaacga
    21961 accatcagca ttattgaccg caatatcctg ttcatcaggc acgcacttat agcaagtaat
    22021 aatcttcatt gcatctccag aaatcatgaa ggaaacgtcg ttcatatcgg tggtgttaat
    22081 tacagatatc acgcccttta atttggaaat aaaactttaa tcaccaatat tgaaaatgtc
    22141 acacgcagat aaatccaact ttcaatattg ttaagctcct caccaatatt gaaaacatgg
    22201 cgtagaaaaa agaaattttc aatattgttt tatggatcac caatattgaa agtcagatgc
    22261 tattttttta ctcacaacag aacataacaa actgattaat aatcaaaatt aacgaaaaaa
    22321 tgccctctat tcacccgaaa caaaaatgtg ataacaatca cagaatgcag cttattgaat
    22381 acccattatg agttagccat taacgcgtcc acgaggttaa taataattat attaaatgtt
    22441 aacaaaaata aaacaaacgg gaacgcaaaa taaatgttcg ttttcacagt ggaattaact
    22501 catgaagaat gaaaagagaa aaacgggaat agaaccgaag gttttctttc cgccgttaat
    22561 aatcgtcggc atactttgtt ggcttacagt cagagatctg gatgcggcga atgtcgttat
    22621 taatgctgta ttcagttacg tcaccaatgt atggggatgg gcatttgaat ggtatatggt
    22681 ggtgatgctt ttcggctggt tctggctggt gtttggcccg tatgccaaaa agcgtttagg
    22741 taacgaacca ccagaattta gtaccgccag ttggatcttt atgatgttcg cctcctgtac
    22801 gtctgctgcc gtactgttct ggggatcgat tgagatctac tactacatct ccaccccgcc
    22861 gtttggctta gaaccgaact caacaggcgc gaaagagttg gggctggctt acagcttgtt
    22921 ccactgggga cctctgccgt gggccactta cagcttcctt tcagtcgcct tcgcttactt
    22981 cttctttgtc cgcaaaatgg aagtgattcg ccccagctcc accctggtgc cgctggtagg
    23041 tgaaaaacac gccaaagggt tgttcggcac tatcgtcgac aacttctatc tcgtcgcctt
    23101 gatcttcgcg atgggtacca gtctgggcct tgccacgccg ctggtgaccg agtgtatgca
    23161 atggttgttt ggcattccgc ataccctgca actggacgct atcatcatta cctgctggat
    23221 tatcctcaac gccatttgcg tcgcttgcgg tctgcaaaaa ggggtacgta tcgccagtga
    23281 cgtgcgtagt tacctgagct tcctgatgct gggttgggtg ttcatcgtca gcggtgccag
    23341 cttcatcatg aactacttca ccgattcggt ggggatgttg ctgatgtatc tgccgcgcat
    23401 gttgttctat accgatccca tcgctaaagg cggcttcccg cagggctgga ccgtgttcta
    23461 ctgggcatgg tgggtgattt acgctatcca gatgagcatc ttcctcgccc gcatctcccg
    23521 tggtcgtacc gtgcgtgaac tgtgcttcgg catggtgctg gggctgacag cgtcaacctg
    23581 gatcctgtgg actgtactcg gtagtaacac tctgctgttg atagataaaa acatcatcaa
    23641 cattccaaat ctgatcgaac agtacggtgt ggcgcgcgcc atcatcgaaa cctgggccgc
    23701 tctgccactc agcaccgcca ccatgtgggg cttcttcatc ctctgcttta ttgccaccgt
    23761 tacgctggtt aacgcctgct cttataccct ggcgatgtcc acttgccgcg aagtacgcga
    23821 tggtgaagaa ccaccgctgc tggtgcgtat cggctggtca attctggttg gcattatcgg
    23881 tattgttctg ctggcgctcg gcggcctgaa accgattcaa accgccatta tcgccggagg
    23941 atgcccgctg ttcttcgtca acattatggt gacgctctcc tttattaaag acgcgaaaca
    24001 gaactggaaa gattaattaa cccccaaaat atcaagaggt tgaaagatgg attttaattt
    24061 aaatgatgag caggaactgt ttgtcgccgg tatccgcgaa ctgatggcca gcgaaaactg
    24121 ggaggcctat tttgccgagt gcgaccgtga cagcgtctac ccggaacgtt ttgtcaaagc
    24181 actggcggat atgggtatcg acagtctgct gattcctgaa gagcacggtg gtcttgacgc
    24241 gggttttgtt actctcgccg ccgtgtggat ggagctggga cgtctggggg caccaaccta
    24301 tgtgctgtac cagttgccgg gcgggttcaa taccttcctg cgcgaaggca cacaagagca
    24361 gatcgacaag attatggctt tccgcggcac cggtaagcag atgtggaact cagcgattac
    24421 cgaaccgggc gcgggctccg acgtgggtag cctgaaaacg acttataccc gtagaaatgg
    24481 taagatttat cttaatggta gtaagtgttt tattaccagc agcgcctaca ccccgtacat
    24541 cgtggtgatg gcgcgcgacg gggcttctcc ggacaaacct gtctacaccg aatggtttgt
    24601 tgatatgagc aaaccgggca tcaaagtgac caaacttgag aagctcggtc tgcgtatgga
    24661 tagctgctgt gaaatcactt ttgacgacgt ggaactggac gagaaagaca tgttcggtcg
    24721 ggaaggtaac ggatttaacc gcgtcaaaga agagttcgac catgaacgtt tcctggtagc
    24781 cctgaccaac tacggtacgg cgatgtgcgc ctttgaagat gcggcgcgct acgccaacca
    24841 gcgcgtgcag tttggcgagg ctattggtcg tttccagttg attcaggaaa aattcgccca
    24901 catggcgatc aaattaaact ccatgaaaaa catgctgtat gaagcagcgt ggaaagcaga
    24961 caacggcacc atcacctctg gcgatgcagc gatgtgcaaa tacttctgcg ccaatgcggc
    25021 atttgaagtg gtggatagcg caatgcaggt gctgggcggt gtcgggattg cgggcaacca
    25081 ccgcatcagc cgcttctggc gtgacctgcg tgtagaccgc gtttccggag gatctgacga
    25141 aatgcagatc ctgacgctgg gtcgcgcggt gctgaagcaa taccgttaag tccgtgcgct
    25201 gcctgatgcg acgctgacgc gtcttatcag gcctacggga gcacgaagat ggccggataa
    25261 ggcgtttacg ccgcatccgg cagtcatgcg ctacataaat ttttcaggag ctaataatgg
    25321 atcatctacc catgccgaaa ttcgggccgc tggccggatt acgcgttgtc ttctccggta
    25381 tcgaaatcgc cgggccgttc gccggacaaa tgttcgcaga atggggggcg gaagttatct
    25441 ggatcgagaa cgtcgcctgg gccgacacca ttcgcgttca accgaactac ccgcagctct
    25501 cccgccgcaa tttgcatgcg ctgtcgttaa atattttcaa agatgaaggg cgcgaagcgt
    25561 ttctgaaatt aatggaaacc accgatatct tcatcgaagc cagtaaaggt ccggcctttg
    25621 cccgtcgtgg cattaccgat gaagtcctgt ggcagcacaa cccaaaactg gttatcgctc
    25681 acctttccgg ttttggtcag tatggcaccg aggagtacac caatcttccg gcctataaca
    25741 ctatcgccca ggccttcagt ggttacctga ttcagaacgg tgatgttgac cagccaatgc
    25801 ctgccttccc gtataccgcc gattactttt ctggcctgac cgccaccacg gcggcgcttg
    25861 cagcactgca taaagtgcgt gaaaccggca aaggcgaaag catcgacatc gccatgtatg
    25921 aagtgatgct gcgtatgggc cagtacttca tgatggatta cttcaacggc ggcgaaatgt
    25981 gcccgcgcat gaccaaaggt aaagatccct actacgccgg ttgcggtctg tataaatgcg
    26041 ccgacggcta catcgtgatg gaactggtgg gcattactca aattgcagag tgctttaaag
    26101 atattggcct cgcacacctg ctcggtacgc cggaaatccc ggaaggcact cagcttatcc
    26161 accgtatcga atgcccttac ggcccactgg tagaagagaa actcgatgcc tggctggcgg
    26221 cacataccat cgcagaagtt aaagaacgct ttgccgaact gaatatcgcc tgtgccaaag
    26281 tactgaccgt accggaactg gaaagcaatc cacagtatgt ggctcgcgaa tcaatcactc
    26341 agtggcaaac gatggatggt cgcacctgca aagggccgaa tatcatgccg aaattcaaaa
    26401 ataaccccgg acaaatctgg cgcggaatgc cctcacatgg catggacacg gctgctattt
    26461 tgaaaaatat cggctacagc gaaaacgaca ttcaggagtt ggtcagcaaa ggtctggcca
    26521 aagttgagga ctaaacgcgt ggcgtcagtc agcttgcctg actagcgccc gctcaataac
    26581 aaacgaacaa aatggataga ggtgcaatgg atatcattgg cggacaacat ctacgtcaaa
    26641 tgtgggacga tctggcggac gtttacggtc ataaaacggc gctgatttgt gaatccagca
    26701 gcggagtcgt taaccggtat agttatcttg agttaaatca ggagattaac cgcacggcaa
    26761 acctgtttta tgtgctgggg attcgcaaag gcgacaaggt tgctctacat ctcgacaact
    26821 gcccggaatt tatcttttgc tggttcgggt tggcaaaaat tggcgcgatt atggtgccga
    26881 ttaacgcccg cctgttgcgc gaagaaagca cgtggatcct gcaaaatagc caggcgtgcc
    26941 tgctggtgac cagtgcgcaa ttttatccta tgtatcaaca gattcagcag gaagatgcct
    27001 ctcagttgcg gcacatttgc ctgacagata tggtacttcc cgctgatgat ggcgtgagtt
    27061 cgtttactca acggaaaaat caacaacctg ccaccttgtg ctatgcaccg ccgctatcga
    27121 ctgacgatac ggcggaaatt ctcttcacct ccggcaccac ctcccgaccg aaaggggtgg
    27181 tgataactca ttacaacctg cgcttcgctg gatattactc cgcctggcag tgtgcactgc
    27241 gtgacgatga cgtctacctg acggtaatgc ctgcgtttca tatcgattgc cagtgtactg
    27301 cggcgatggc ggcgttttct gccggggcca cctttgtgct ggtcgagaaa tacagcgccc
    27361 gcgccttctg gggacaggtg cagaagtacc gcgccaccat taccgaatgt attccgatga
    27421 tgattcgtac gttgatggtg cagccgcctt cagccaacga tcggcaacac tgcctgcggg
    27481 aagtgatgtt ttatctcaac ctgtcggagc aggaaaaaga cgcgttttgt gaacgcttca
    27541 gcgttcgctt gctgacgtct tatgggatga cggaaaccat tgtgggcatt atcggcgatc
    27601 gccctggcga taaacggcgc tggccgtcga ttggtcgggc gggcttttgc tacgaagcgg
    27661 agatccgcga cgatcacaat cgtcccctcc cggctggtga gctcggtgaa atctgtatta
    27721 aaggcgtacc agggaaaacc atcttcaaag agtattttct caacccgaaa gccactgcga
    27781 aagtgctgga agccgatggc tggctacata ccggcgatac cggataccgc gacgaagagg
    27841 gcttttttta tttcgtcgat cgccgctgca atatgatcaa acgcggtggc gagaatgtct
    27901 cctgcgtgga gctcgaaaat atcatcgcca cccatccaaa aattcaggat atcgtggttg
    27961 tgggtattaa agattcgatt cgcgatgaag ccatcaaagc atttgtggtg ctgaatgaag
    28021 gtgaaacatt gagcgaagag gaattcttct gcttctgtga acagaatatg gcgaaattta
    28081 aagtgccctc ttatctggag atcagaaaag atctgccacg taattgctcg gggaaaataa
    28141 ttagaaagaa tctgaaataa atgaaacggc agggaactac cctgtcagct aataatcacg
    28201 ctctaaaaca atatgcattt tttgcaggga tgttgtcgtc cctgaaaaag caaaaatgga
    28261 gaaaaggaat gagtgaatca ttacatctga cccgcaatgg atcaattctg gaaattaccc
    28321 ttgatcgtcc aaaagcgaat gctattgatg caaaaaccag ctttgaaatg ggcgaagtat
    28381 ttctaaattt ccgtgacgat ccgcaattac gtgtcgccat tattaccggt gccggagaga
    28441 aattcttttc cgcgggctgg gatttaaaag cggcagcaga aggcgaagca ccggatgctg
    28501 actttggccc gggaggtttt gcgggattaa ccgaaatttt caatctcgac aaaccggtta
    28561 tcgcagctgt gaacggctac gcctttggcg gcggtttcga actggcgctg gcggcagatt
    28621 ttattgtttg tgccgataat gccagcttcg ccctgccgga agccaaactg ggcatcgttc
    28681 ctgacagcgg cggcgtgctg cgtctgccga agatcctgcc gcctgccatc gtcaatgaaa
    28741 tggtgatgac cggcagacga atgggcgcag aagaggcgca gcgttggggg atagtcaacc
    28801 gcgtggttag ccaggcggaa ctgatggata acgcccgcga actggcgcag cagctggtta
    28861 acagcgctcc actggcgatt gcggcgctga aagagatcta ccgcaccacc agcgaaatgc
    28921 cggtagaaga agcgtatcgc tatattcgca gcggcgtgtt gaaacactat ccatcggttc
    28981 tgcattcgga agatgccatt gaagggccgc tggcgtttgc cgagaagcgc gatccggtgt
    29041 ggaaaggacg ttaacaaccg tgagctatta cgcctttgag ggattaattc cggtggttca
    29101 cccgacggcg tttgtccatc ccagtgcagt attgattggc gatgtgattg tgggagccgg
    29161 tgtctacatc ggcccactcg cctcactgcg tggtgactac gggcggttga tcgtgcaagc
    29221 gggagccaat attcaggatg gctgcattat gcatggctac tgcgacactg acaccatcgt
    29281 tggggaaaac ggccatatcg ggcacggagc gatcctgcat ggttgtgtga ttggtcgcga
    29341 tgcactggtc gggatgaaca gcgtgattat ggatggcgcg atcattggcg aagagagcat
    29401 tgttgccgct atgagctttg tcaaagcggg ctttcgcggc gagaaacgcc agttgttgat
    29461 gggtacgcca gcccgcgctg tacgcagcgt gagtgacgac gagttacact ggaaacggtt
    29521 gaataccaaa gagtataagg atcttgtcgg gcgctgtcat gcatcattac atgaaacgca
    29581 gccactgagg caaatggagg aaaatcgccc ccgtttgcag gggacgacgg atgtgacgcc
    29641 gaaacggtga tctttccgga tgcggcgtaa acgccttatc cgacctggct ggaaaggaac
    29701 cgtaggccgt ataagatgcg ccagcatcgc atccggcgac caggtcgcac ggtatcaacg
    29761 aaacgacatt aacgacgcat actctttgac aacatcatct gccacttctg ctcgcggtta
    29821 agttccgtga gcggaacggc aggaatacgt ggcgtctttc ttacgccgac taatttttcc
    29881 cgactgttat tgcgcaggcg cgcgtaaatt tgctcatcga tatcgaccac tttaaccagt
    29941 cgctgacact ggcatccccg cccttccagc ttattaggga tcatcttaac ttcgcagctt
    30001 atttctgtga cttccgacag aatataagtc agggtattaa ccgccttgct gtgttcaata
    30061 tcgaaatgaa tagagatctc tcttgctatc acccaccgat tttcagccat catccattcg
    30121 gcgattaaca agtagagtgg tttttcaaca tatccttcac acatacgaac tccagtttca
    30181 caaattaaaa gtctggctat gactcgtttt gcagcaatta aacaaggcca tattaaaatt
    30241 gtggatatat cgatagtatg caacttcacc gtcgataagt gtgagctaaa tcctgcttat
    30301 gtcatcaaaa atatcgattc cccaaaagat ttacttaatg ggcaaaaaaa acggtcctca
    30361 tcagagaacc gtattagtta ttcgatcaaa ttagcggatg aaaaatatcc gccatgacac
    30421 gctattattt gatctgcgcg tgcatttcct gcaccgaaat tactttttca gtcgcatcgg
    30481 cattcagcgc catcgcggta gcgaaaccac cgttcagggt ggtgtcgtaa tgcactttat
    30541 attgcagcgc actgcgacgg atcacgcggg agtcttcaat cgcacgacga cctgaggtgg
    30601 tgttgatgat gtaggtatat tcgccattct tgatacggtc ctgaatgtgc ggacgacctt
    30661 catgcacctt gttcaccaga cgcggattaa tgcctgcttc gcccagcaca atcgccgtgc
    30721 cgtgggtcgc atccagctcg aagccctgtt tcagcagttt tgccgccagg tccaccacgc
    30781 gttctttatc gccttcgcgc acggaaagca acgcacgacc gtgtttcttc atggtggagt
    30841 tgctgcccag ctgcgctttg gcgaacgctt cagcgaaggt gcggcccacg cccatgactt
    30901 ccccggtaga gcgcatttct ggccctaaca gcgggtcaac gcccgggaat ttattgaacg
    30961 gcagcaccac ttctttcacc gagtagtacg gcgggataac ttctttggtt acgccctgct
    31021 cagccagcga tttgccagcc atcacacgcg ccgccacttt tgccagcggt acgccggtgg
    31081 ctttggagac gaacggaacg gtacgcgccg cacgcgggtt aacttcaatc aggtagactt
    31141 cgttgttttt caccgcaaac tgcacgttca tcaggccgcg cacctgcaat tcgaaggcca
    31201 gtttctgcac ctgctggcgc atcacatcct gaatttcctg acttaaagtg taggctggta
    31261 gcgaacatgc agagtcaccg gagtgcacgc ccgcctgctc gatgtgctcc atgatgccgc
    31321 caatcagcac catttcaccg tcgcagatgg catccacgtc aacttctact gcgtcatcaa
    31381 ggaagtggtc cagcaacact ggcgcatcgt tagatacgct gaccgctgtc tggaagtagc
    31441 gacgcaggtc agcttcgtca tagacgattt ccatcgcccg accgccgaga acgtaagacg
    31501 gacgcaccac cagcgggtag ccaatctctt tcgctttctc aaccgccatt tcgatggtgg
    31561 taacggtggc gttcgccggt tgtttcagtt tcagacgctc aaccgcatgc tggaagcgtt
    31621 cacggtcttc cgcacggtcg atggcatccg ggctggtacc gataaccggt acgccagcag
    31681 cttccagcgc gcgcgccagt ttcagcgggg tctgaccgcc gtactggacg ataacgcctt
    31741 tcggcttctc aatacgcaca atttccagca cgtcttccag agttaccggc tcgaagtaga
    31801 gacggtcaga ggtgtcgtag tcggtggaga cggtttccgg gttacagtta accataatgg
    31861 tttcataacc atcttcgcgc agcgccagcg aggcgtgtac acagcagtag tcgaattcga
    31921 taccctgacc gatacggttc gggccgccgc cgagcaccat gattttttca cggtcggtag
    31981 acggattcgc ttcgcactct tcttcataag tggagtacat gtaagcggtg tcggtggcga
    32041 actctgccgc acaggtatcc acgcgcttat aaaccgggtg caagtcatac tggtcgcgca
    32101 gcttgcggat ttctgcttca cgcacgcccg cgagttttgc cagacgcgcg tcggcaaagc
    32161 ctttgcgttt cagctggcgc aggaagtcag cgttcaggcc agtgatgccc acttctgcga
    32221 ctttctcttc cagacgcacc agctcttcaa tttgtaccag gaaccagcgg tcaatgttgg
    32281 tcaggttgaa cacgccgtct acggacaggc ccgcgcggaa agcatcggcg atataccaga
    32341 tacgctctgc gcctgcatct ttcagttcgc ggcgaatttt ggtcagcgct tccggatcat
    32401 cgaggctgac tttcgggtcg aagccagttg cgcccacttc caggccgcgc agcgcttttt
    32461 gcagggattc ctgctgcgtg cgaccaatcg ccatcacttc gccaaccgat ttcatctgag
    32521 tggtcagacg gtcgttcgcc ccggcgaatt tctcgaagtt gaagcgagga attttagtca
    32581 ccacatagtc gatggacggc tcgaaggagg ccggagtacg cccaccggtg atgtcgttca
    32641 tcagctcgtc gagggtgtaa cccaccgcca gtttcgccgc cactttggca atcgggaaac
    32701 cagtggcttt cgacgccagc gcagaggagc gagacacgcg cgggttcatt tcgataacaa
    32761 tcaggcgacc gtttttcggg ttcaccgcaa actggacgtt ggaaccaccg gtttcaacgc
    32821 cgatttcacg cagtaccgcc atcgaggcgt tacgcatgat ttgatattct ttgtcggtca
    32881 gcgtttgggc tggcgcgaca gtgatggagt caccggtgtg gatgcccatc gcatcaaagt
    32941 tttcgataga gcagacaatg atgcagttgt cgtttttatc acgcaccact tccatctcgt
    33001 actctttcca gccgatcagc gactcatcaa tcagcagctc tttagtcgga gagagatcca
    33061 gaccacgggc gcaaatttct tcaaactctt cgcggttata agcgataccg ccgccgctac
    33121 cgcccatggt aaaggatggg cgaataatgc acgggaagcc aacttcagcg gcaaccgcca
    33181 gcgcttcttc cattgaatga gcgataccgg aacgcgcggt ttccagaccg attttcttca
    33241 tcgctacgtc gaaacggcgg cggtcttcgg ctttatcaat cgcatcggca gtggcaccaa
    33301 tcatggtgac gccgaactct tccagcacgc cctgacgctc aagctcaagc gcacagttca
    33361 gcgccgtctg cccccccatg gttggcagca ccgcgtccgg acgctctttt tcaataatct
    33421 tgcgtaccac ttcccagtga atcggttcga tgtaggttgc atcagccatt tccgggtcgg
    33481 tcatgatggt cgccgggttg gagttcacca gaatgacgcg gtaaccctct tcacgcaggg
    33541 ctttacacgc ttgcgcgcca gagtagtcaa actcacacgc ctgaccgata acaatcgggc
    33601 ccgcacccag aatcaggata ctttttatat ctgtacgttt tggcatggct cttttactcc
    33661 tgattactta gcggttttac ggtactgctc aattaactcg ataaagtggt caaacaacgg
    33721 cgcggcgtcg tgtggaccag ggctggcttc cgggtgtccc tggaagctga acgccggttt
    33781 atcggtacga tgaatgccct gtaacgtacc gtcgaacagg gatttatgtg tgacacgcag
    33841 gtttgcaggt aatgttgctt catccaccgc aaaaccgtgg ttctgggcgg taatcatcac
    33901 cacatttttc tcaacatctt taaccggatg gttgccgccg tggtgaccaa atttcatttt
    33961 tacagtcttc gcaccgctcg ccagcgccag cagctgatga ccgaggcaga tgccgaatac
    34021 cggaatatcg gtttcgagga atttctggat ggcggtaatg gcgtaatcgc acggggccgg
    34081 gtcgccagga ccgttggaga ggaagatgcc gtctggattc attttcagca cgtcttccgc
    34141 agaagtttgc gccggaacga tggtcagacg acagcctcta tccaccaaca tgcgcaggat
    34201 gttgcgcttg gcaccaaaat cataagccac aacatggaac ggcagctcgt cttctttctt
    34261 cgcttctggc aggccgccgg tcaacgtcca gctcccttgt gtccagctat aggcttctgc
    34321 ggtggtcact tcttttgcca gatccatgcc gttcagacct gggaacgcgc gggctttttc
    34381 taatgccagc gccgcatccg ggttatcgcc tgcgataatg cagccattct gtgcgccttt
    34441 ctcgcgcagt aaacgcgtca gcttacgggt atcgatatcg gcaatcgcca cgatgttatg
    34501 gcgcttcagg taagaagaga ggtcttcggt attacggaag ttgctggcaa tcagcggcag
    34561 gtcgcgaatc accagacctt gtgcatgtac ctgagaagat tcttcatcgg cgtcattagt
    34621 gccgacatta ccaatatggg gataagtaag agtaacgatt tgacgagaat aggaaggatc
    34681 agtgaggatt tcttgataac cggtcattga agtattgaaa acgacttccc caaccgccga
    34741 acctgttgcc cctatggccc gaccgtgaaa ctgggttccg tcttccagaa ccaatagcgc
    34801 tgacttaatc aaaacaccct ccagagaata ttcactcact ttatttgcat attaattcac
    34861 aatgatgacg taaatcaatg caaatctgct taccgacgaa tttctggcaa acgg
//
LOCUS       DAZGQL010000055        34243 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.055, whole genome shotgun sequence.
ACCESSION   DAZGQL010000055 DAZGQL010000000
VERSION     DAZGQL010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34243
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.055"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..1171
                     /locus_tag="ACHKIR_002866"
     CDS             <1..1171
                     /locus_tag="ACHKIR_002866"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFO9990576.1"
                     /translation="NNTVLVSINGTIGNTALYNNENIILGKSACYINLKDNISKHFIL
                     YVLSGYLFQEYIQRCSTGSTIKNVSLKMMRDFRFLMPESKEEQEKVVRIIQKIDELKR
                     LNNAQNQTLEQMFQALFKSWFVDFDPVIDNALDAGNPIPETLQSRAELRQNVRNSTDF
                     KPLPAEIRSLFPSEFEETELGWVPKGWESETFDSFCDLIQSGGTPSRKETSFWDGGTI
                     KWLSSGEVKGKIVLDTKEKITDTGLLNSSSKLWEKYTTVVAMYGATAGEVCIIGDKMA
                     ANQACCGLYSKTFPFFVYNFVCNKANELASKATGSAQQNLNKLIISTTKFICPSNDII
                     TIFEDNVTPLFMKWFSNSSENNTLTALRNTLLPKLISGELSVEDLPDHIKQTEAA"
     gene            1266..4529
                     /locus_tag="ACHKIR_002867"
     CDS             1266..4529
                     /locus_tag="ACHKIR_002867"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HFO9990577.1"
                     /translation="MSLSFSEAKLEQAIIELLQDQGYQHLIGDNVPRSSLDQVIIEDD
                     LRHYLAARYQPDGITEEEIQRLIKQFTTLPASDLYESNKTFCRWLANGFLFKRDDRQQ
                     KDLYIELLDTRHLPAALRQIFDAEDVPLQQAAELPPSYINPPLNRIKIVNQLKISGKD
                     NQTRIPDGILYINGLPLVVFEFKSAVREQEANIGDAWKQLCKRYRRDIPQLFIYNALC
                     IISDGVNNRMGNLFAPYEYFYSWRKVTGNENREQDGIPSLHSMIQGLFHPVRLLDVIK
                     NFICFPDKAKHEVKICCRYPQYYAARKLYYSIKQARKPFGSGKGGTYFGATGCGKSYT
                     MQFLTRLLMKSVEFASPTIVLITDRTDLDDQLSAQMCNAKNYIGDDTIVPVTSREDLR
                     NQLAGRNSGGVFLTTIHKFTEDTELLSERSNIICISDEAHRSQVNLDQKVIVDKESGK
                     VRKTYGFAKYLHDSLPNATYVGFTGTPIDATLDVFGEVIDSYTMTESVQDEITVRIVY
                     EGRAAKVILDSSKLEEVEKYYEECANAGTNEWQIDESKKATATMNAVLGDEDRLKALA
                     EDFAKHYEKRVAEGSTVIGKAMFVCASREIAWDFYRQLKAIRPAWFEVKQAPDGVVLT
                     EQEQKELPPSEMVKMVMTRGKDDDEALYDLLGTKEYRKELDKQFKNAKSNFKIAIVVD
                     MWLTGFDVPELDTIYIDKPLQKHNLIQTISRVNRKLEGKSKGLVVDYIGIKSQMNQAL
                     AMYSRIDATNFEDIQQSVIEVKNHLDLLGQVFYEFDSRDYFSGEPQAQLSCLNRAAEF
                     VLRTQKVERRFMGLVKRMKAAYDVCCGSEALSQTERDHIHYYLAVRSIVFKLTKGDAP
                     DVTQMNARVREMIAEALKADGVEEIYFLGDKKAESIDIFDEDYLARINKIKLPATKIQ
                     LLQKLLEKAISDFKKVNQLQGINFTRRFQAIIDRYNERREDDVLNGEEFDTFSQEMTD
                     IIYDIKTEMGTWADLGIDIEEKAFFDILAHMRDKYQFTYDDEKMLSLAKEMKSVVDNT
                     SKYPDWSKRDDIKAKLKVELILLLHKHKFPPVANDDVYMGVLAQAENFKEHHMSALN"
     gene            4744..5082
                     /gene="symE"
                     /locus_tag="ACHKIR_002868"
     CDS             4744..5082
                     /gene="symE"
                     /locus_tag="ACHKIR_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001534835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HFO9990578.1"
                     /translation="MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLETAGFATGTAVDVKVIEGCIVLTAQPPAAEESELMQSLRQVCKLSARKQRQVQDF
                     IGVISSKTPR"
     gene            complement(5124..5177)
                     /locus_tag="ACHKIR_002869"
     CDS             complement(5124..5177)
                     /locus_tag="ACHKIR_002869"
                     /inference="COORDINATES: protein motif:HMM:NF018294.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HFO9990579.1"
                     /translation="MSDERLRDIGLRREDVE"
     gene            complement(5166..5321)
                     /locus_tag="ACHKIR_002870"
                     /pseudo
     CDS             complement(5166..5321)
                     /locus_tag="ACHKIR_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001684533.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
     gene            5464..6876
                     /gene="yjiR"
                     /locus_tag="ACHKIR_002871"
     CDS             5464..6876
                     /gene="yjiR"
                     /locus_tag="ACHKIR_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFO9990580.1"
                     /translation="MTRYQHLATLLAERIEQGLYRHGEKLPSVRSLSQEHGVSISTVQ
                     QAYQTLETMKLITPQPRSGYFVAQRKAQPPVPPMTRPVQRPVEITQWDQVLDMLEAHS
                     DSSIVPLSKSTPDVETPSLKPLWRELSRVVQHNLQTVLGYDLLAGQRVLREQIARLML
                     DSGSVVTADDIIITSGCHNSMSLALMAVCKPGDIVAVESPCYYGSMQMLRGMGVKVIE
                     IPTDPETGISVEALELALEQWPIKGIILVPNCNNPLGFIMPDARKRAVLSLAQRHDIV
                     IFEDDVYGELATEYPRPRTIHSWDIDGRVLLCSSFSKSIAPGLRVGWVAPGRYHDKLL
                     HMKYAISSFNVPSTQMAAATFVLEGHYHRHIRRMRQIYQRNLALYTCWIREYFPCEIC
                     ITRPKGGFLLWIELPEQVDMVCVARQLCRMKIQVAAGSIFSASGKYRNCLRINCALPL
                     SETYREALKQIGEAVYQAME"
     gene            complement(7118..8062)
                     /locus_tag="ACHKIR_002872"
     CDS             complement(7118..8062)
                     /locus_tag="ACHKIR_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001678496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFO9990581.1"
                     /translation="MTNFTTSTPHDALFKSFLTHPDTARDFMEIHLPKDLRELCDLDS
                     LKLESASFVDEKLRALHSDILWSVKTREGDGYIYVVIEHQSREDIHMAFRLMRYSMAV
                     MQRHIEHDKRRPLPLVIPMLFYHGSRSPYPWSLCWLDEFADPTTARKLYTAAFPLVDV
                     TVVPDDEIVQHRRVALLELIQKHIRQRDLMGLIDQLVILLVTECANDSQITALLNYIL
                     LTGDEARFNEFISELTRRMPQQRERIMTIAERIHNDGWLLGMEKGKEEGEQRLLRLLL
                     QNGADPEWIQRYTGLSAEQMQALEQPLPESKRDPWIEY"
     gene            8529..9761
                     /gene="mdtM"
                     /locus_tag="ACHKIR_002873"
                     /pseudo
     CDS             8529..9761
                     /gene="mdtM"
                     /locus_tag="ACHKIR_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313327.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtM"
     gene            9802..11082
                     /gene="yjiN"
                     /locus_tag="ACHKIR_002874"
     CDS             9802..11082
                     /gene="yjiN"
                     /locus_tag="ACHKIR_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HFO9990582.1"
                     /translation="MNKLIELRRAKMLALSLLLIAAATFVVTLFLPPNFWVSGVKAIA
                     EAAMVGALADWFAVVALFRRVPIPIISRHTAIIPRNKDRIGENLGQFVQEKFLDTQSL
                     VALIRRHEPALLIGNWFSQPENARRVGQHLLQIMSGFLELTDDARIQRLLKRAVHRAI
                     DKVDLSGTSALMLESMTKNDRHQVLLDTLIAQLIALLQRDKSRKFIAQQIVRWLESEH
                     PLKAKILPTEWLGEHSAELVSDAVNSLLDDISRDRAHQIRHAFDRATFALIDKLKNDP
                     EMAARADAVKSYLKEDEAFNRYLSELWGDLREWLKVDINSEDSRVKERIARAGQWFGE
                     TLIADDALRASLNGHLEQAAHRVAPEFSAFLTRHISDTVKSWDAQDMSRQIELNIGKD
                     LQFIRVNGTLVGGCIGLILYLLSQLPALFPLGNL"
     gene            11198..12349
                     /gene="yjiM"
                     /locus_tag="ACHKIR_002875"
     CDS             11198..12349
                     /gene="yjiM"
                     /locus_tag="ACHKIR_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double-cubane-cluster-containing anaerobic
                     reductase"
                     /protein_id="HFO9990583.1"
                     /translation="MSLVTDLPAIFDQFSEARQKGFLTVMDLKERGIPLVGTYCTFMP
                     QEIPMAAGAVVVSLCSTSDETIEEAEKDLPRNLCPLIKSSYGFGKTDKCPYFYFSDLV
                     VGETTCDGKKKMYEYMAEFKPVHVMQLPNSVKDDASRALWKAEMLRLQKTVEERFGHE
                     ISEDALRDAIALKNRERRALANFYHLGQLNPPALSGSDILKVVYGATFRFDKEALINE
                     LDAMTARVRQQWEEGQRLDPRPRILITGCPIGGAAEKVVRAIEENGGWVVGYENCTGA
                     KATEQCVAETGDVYDALADKYLAIGCSCVSPNDQRLQMLSQMVEEYQVDGVVDVILQA
                     CHTYAVESLAIKRHVRQQHNIPYIAIETDYSTSDVGQLSTRVAAFIEML"
     gene            12359..13126
                     /gene="yjiL"
                     /locus_tag="ACHKIR_002876"
     CDS             12359..13126
                     /gene="yjiL"
                     /locus_tag="ACHKIR_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298028.1"
                     /note="YjiL, as found in Escherichia coli, is a homolog of
                     the activator ATPases of enzymes such as lactoyl-CoA
                     dehydratase, (R)-phenyllactate dehydratase, and
                     2-hydroxyisocaproyl-CoA dehydratase. The typical substrate
                     of those enzymes is acyl-CoA with an OH at the
                     beta-position. YjiL is the putative activator for the
                     cognate protein YjiM, a putative 2-hydroxyacyl-CoA
                     dehydratase with unknown specificity, encoded by the
                     adjacent gene; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-hydroxyacyl-CoA dehydratase activator
                     YjiL"
                     /protein_id="HFO9990584.1"
                     /translation="MTYSIGIDSGSTATKGILLADGVITRRFLVPTSFRPATAITEAW
                     ETLREGLETTPFLTLTGYGRQLVDFADKQVTEISCHGLGARFLAPATRAVIDIGGQDS
                     KVIQLDDDGNLCDFLMNDKCAAGTGRFLEVISRTLGTSVEQLDSITENVTPHAITSMC
                     TVFAESEVISLRSAGVAPEAILAGVINAMARRSANFIARLSCEAPILFTGGVSHCQRF
                     THMLESHLGMPVQTHPDAQFAGAIGAAVIGQRQRKRA"
     gene            13123..13380
                     /gene="ytiA"
                     /locus_tag="ACHKIR_002877"
     CDS             13123..13380
                     /gene="ytiA"
                     /locus_tag="ACHKIR_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HFO9990585.1"
                     /translation="MKEHLFLFHSTVGVIQTRKALQAAGMTFRVSDIPRDLRGGCGLC
                     IWLTCPPGEEIQWVIPGHTESVYCQQDGGWRCIAHYGISPR"
     gene            13445..14305
                     /gene="yjiK"
                     /locus_tag="ACHKIR_002878"
     CDS             13445..14305
                     /gene="yjiK"
                     /locus_tag="ACHKIR_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418753.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HFO9990586.1"
                     /translation="MTKSISLSKRIFVIVILFVIVAVCTFFVQSCARKSNHAASFQNY
                     HATIDGKEIAGITNNISSLTWSAQSNTLFSTINKPAAIVEMTTNGDLIRTIPLDFVKD
                     LETIEYIGDNQFVISDERDYAIYVISLTPNSEVKILKKIKIPLQESPTNCGFEGLAYS
                     RQDHTFWFFKEKNPIEVYKVNGLLSSNELHISKDKALQRQFTLDDVSGAEFNQQKNTL
                     LVLSHESRALQEVTLVGEVIGEMSLTKGSRGLSHNIKQAEGVAMDASGNIYIVSEPNR
                     FYRFTPQSSH"
     gene            14373..15551
                     /gene="yjiJ"
                     /locus_tag="ACHKIR_002879"
     CDS             14373..15551
                     /gene="yjiJ"
                     /locus_tag="ACHKIR_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9990587.1"
                     /translation="MPSSTHPVERFSFSTALFGMLVLTLGMGLGRFLYTPMLPVMMAE
                     GSFSFSQLSWIASGNYAGYLAGSLLFSFGAFHQPSRLRPFLLASALASGLLILAMAWL
                     PPFILVLLIRVLAGVASAGMLIFGSTLIMQHTRHPFVLAALFSGVGIGIALGNEYVLA
                     GLHFDLSSQTLWQGAGALSGMILIALTLLMPSKKHAIVPMPLAKTEQQIMSWWLLAIL
                     YGLAGFGYIIVATYLPLMAKDAGSPLLTAHLWTLVGLSIVPGCFGWLWAAKRWGALPC
                     LTANLLVQAICVLLTLASDSPLLLIISSLGFGGTFMGTTSLVMTIARQLSVPGNLNLL
                     GFVTLIYGIGQILGPALTSMLGNGTSALASATLCGAAALFIAALISTVQLFKLQVVTS
                     "
     gene            complement(15564..16118)
                     /gene="kptA"
                     /locus_tag="ACHKIR_002880"
     CDS             complement(15564..16118)
                     /gene="kptA"
                     /locus_tag="ACHKIR_002880"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HFO9990588.1"
                     /translation="MAKYNDKELAETSKFLSFVLRHKPEAIGIVLDREGWADIDKLIL
                     CAQKAGKRLTRALLDIVVATSDKKRFSYSSDGTCIRAVQGHSTSQVAIGFIEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGERHYVHLSADEATARKVGARHGSPVILTVKAQEMAKR
                     GIPFWQAENGVWLTSTVAVEFLEW"
     gene            16368..17051
                     /gene="yjiH"
                     /locus_tag="ACHKIR_002881"
     CDS             16368..17051
                     /gene="yjiH"
                     /locus_tag="ACHKIR_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HFO9990589.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGAGGTGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            17048..17509
                     /gene="yjiG"
                     /locus_tag="ACHKIR_002882"
     CDS             17048..17509
                     /gene="yjiG"
                     /locus_tag="ACHKIR_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HFO9990590.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATAGALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            17522..18694
                     /gene="iadA"
                     /locus_tag="ACHKIR_002883"
     CDS             17522..18694
                     /gene="iadA"
                     /locus_tag="ACHKIR_002883"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFO9990591.1"
                     /translation="MIDYSAAGFTLLQGAHLYAPEDRGICDVLIANGKIIAVASNIPS
                     DIVPDCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALQPVYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQMLVKDYSFSLSDALRPLTS
                     SVAGFLNLTGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            18759..19670
                     /gene="hypT"
                     /locus_tag="ACHKIR_002884"
                     /gene_synonym="qseD"
     CDS             18759..19670
                     /gene="hypT"
                     /locus_tag="ACHKIR_002884"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001677288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HFO9990592.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMGRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNREELVIPIQAY
                     AYRMNTRMNPIAERFWRELRELEIEPG"
     gene            19677..20048
                     /locus_tag="ACHKIR_002885"
                     /pseudo
     CDS             19677..20048
                     /locus_tag="ACHKIR_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519422.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative PEP-binding protein"
     gene            complement(20096..20479)
                     /gene="iraD"
                     /locus_tag="ACHKIR_002886"
     CDS             complement(20096..20479)
                     /gene="iraD"
                     /locus_tag="ACHKIR_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001695400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HFO9990593.1"
                     /translation="MMRQSVQTVLPESTGHNTLSLRDSVCRDLFQLFSSPHSPLPILL
                     VSGMPEWQGHNQSDKLLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHELAISLEMM
                     LYHDDEPLTFDLVWQKGNWHRTMPQ"
     gene            complement(20893..21666)
                     /gene="uxuR"
                     /locus_tag="ACHKIR_002887"
     CDS             complement(20893..21666)
                     /gene="uxuR"
                     /locus_tag="ACHKIR_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HFO9990594.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(21881..23341)
                     /gene="uxuB"
                     /locus_tag="ACHKIR_002888"
     CDS             complement(21881..23341)
                     /gene="uxuB"
                     /locus_tag="ACHKIR_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HFO9990595.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPAYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(23422..24606)
                     /gene="uxuA"
                     /locus_tag="ACHKIR_002889"
     CDS             complement(23422..24606)
                     /gene="uxuA"
                     /locus_tag="ACHKIR_002889"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFO9990596.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAV
                     RFATMSDEDKARLTRNIIVGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            24946..26289
                     /gene="gntP"
                     /locus_tag="ACHKIR_002890"
     CDS             24946..26289
                     /gene="gntP"
                     /locus_tag="ACHKIR_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HFO9990597.1"
                     /translation="MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(26462..27364)
                     /gene="fimH"
                     /locus_tag="ACHKIR_002891"
     CDS             complement(26462..27364)
                     /gene="fimH"
                     /locus_tag="ACHKIR_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFO9990598.1"
                     /translation="MKRVIYLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPAVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(27384..27887)
                     /gene="fimG"
                     /locus_tag="ACHKIR_002892"
     CDS             complement(27384..27887)
                     /gene="fimG"
                     /locus_tag="ACHKIR_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HFO9990599.1"
                     /translation="MKWRKRGYLLAAMLALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTAGFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNSGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(27900..28430)
                     /gene="fimF"
                     /locus_tag="ACHKIR_002893"
     CDS             complement(27900..28430)
                     /gene="fimF"
                     /locus_tag="ACHKIR_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HFO9990600.1"
                     /translation="MRNKPFYLLCAFLWLAVSHALAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAVKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAAAGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(28440..31076)
                     /gene="fimD"
                     /locus_tag="ACHKIR_002894"
     CDS             complement(28440..31076)
                     /gene="fimD"
                     /locus_tag="ACHKIR_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HFO9990601.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLFVACAFAAQAPLSS
                     AELYFNPRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVSGMNLLADDACVPLTSMIHDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIG
                     AWRLRDNTTWSYNSSDSSSGSKNKWQHINTWLERDIIPLRSRLTLGDGYTQGDIFDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQVTIKQNGYDIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMGALGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRSSTLYLSGSHQTYWGTSNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANI
                     GYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCV
                     ANYQLPPESQQQLLTQLSAECR"
     gene            complement(31143..31868)
                     /gene="fimC"
                     /locus_tag="ACHKIR_002895"
     CDS             complement(31143..31868)
                     /gene="fimC"
                     /locus_tag="ACHKIR_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418736.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFO9990602.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKRENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENTLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(31905..32444)
                     /gene="fimI"
                     /locus_tag="ACHKIR_002896"
     CDS             complement(31905..32444)
                     /gene="fimI"
                     /locus_tag="ACHKIR_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO9990603.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     VEAGDKQMTVNMGKISSNRFHAVGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIATNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(32509..33063)
                     /gene="fimA"
                     /locus_tag="ACHKIR_002897"
     CDS             complement(32509..33063)
                     /gene="fimA"
                     /locus_tag="ACHKIR_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFO9990604.1"
                     /translation="MKIKTLAIVVLSALSLSSTAALAETTPTTVNGGTVHFKGEVVNA
                     ACAVDAGSVDQTVQLGQVRTASLAQEGATSSAVGFNIQLNDCDTTVADKAAIAFLGTA
                     IDGTHPNVLALQSSAAGSATKVGVQILDRTGAALALDGATFSAQTTLNNGTNTIPFQA
                     RYYAIGEATPGAANADATFKVQYQ"
     gene            complement(33544..34140)
                     /gene="fimE"
                     /locus_tag="ACHKIR_002898"
     CDS             complement(33544..34140)
                     /gene="fimE"
                     /locus_tag="ACHKIR_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HFO9990605.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
BASE COUNT         8358 a         8617 c         8389 g         8879 t
ORIGIN      
        1 taataatacc gttttagttt caataaatgg aaccatcggg aatactgcat tatataataa
       61 tgaaaacata attttgggta aaagtgcatg ttatatcaat ctaaaagata atattagcaa
      121 acactttata ctatatgtac tttctggtta tttatttcaa gagtatatcc aaaggtgttc
      181 aactgggagt acaattaaaa acgtatcact aaaaatgatg cgtgatttca ggtttttaat
      241 gcctgaatca aaagaggagc aggaaaaggt tgttcgtata atacaaaaaa ttgatgaact
      301 aaaaagatta aataatgctc aaaatcaaac cctggaacaa atgttccaag ccctgttcaa
      361 atcctggttt gtagattttg atccggtaat tgataacgct ctggatgcag gaaatccaat
      421 cccggaaacc ctgcaatctc gtgccgagtt gcgtcaaaat gtacgtaata gtacagattt
      481 caaaccgctt ccggcggaaa ttcgctcgct tttcccaagt gaatttgaag aaacggagtt
      541 gggttgggtg ccaaagggtt gggaaagtga aactttcgat tcattctgcg acttaattca
      601 gagtggggga acgccttcaa ggaaggaaac ttcattctgg gatggcggta caataaaatg
      661 gctttcatct ggcgaagtga aaggaaaaat agtcttagat acaaaagaaa aaatcacaga
      721 tactggatta ctaaatagtt catctaaatt atgggaaaaa tatacaaccg ttgttgcaat
      781 gtacggtgcg actgcgggtg aagtgtgtat aattggtgat aaaatggcgg ccaatcaagc
      841 atgttgtgga ttgtattcaa agacattccc attttttgtc tataattttg tctgcaataa
      901 agcaaatgaa ttagcatcaa aagctacagg ttcagcgcaa cagaacctca ataaattaat
      961 tatatctaca acaaaattta tatgcccctc aaatgatata attactatct ttgaagataa
     1021 tgtaacccca ttatttatga aatggttttc taattcgtct gaaaataata ccctaacagc
     1081 cctccgcaat accctgctcc ccaaactcat ctccggcgag ctatccgtgg aggatctccc
     1141 ggatcacatc aaacaaaccg aagccgcata acgcattttg cccctgtaaa atcaggggct
     1201 ttctggtaag gttttctact gatacgggaa tgcttaccag aaattagcca gggttggagc
     1261 acgatatgag tctctctttc agtgaagcaa aattagaaca agcgatcatt gaactgttac
     1321 aggatcaggg atatcaacac ctgatcggcg ataatgtccc acgttcaagt ctcgatcagg
     1381 tcattatcga agacgatctc cgtcattatt tagcggcacg ctaccagcct gatggcatta
     1441 ctgaagaaga gattcagcga ctgatcaaac agttcaccac gcttccggcc tccgatcttt
     1501 atgaaagcaa caaaacattt tgtcgctggc tggcaaatgg ttttctgttc aaacgcgacg
     1561 atcggcaaca aaaagatctc tatattgaat tgctcgacac ccggcatcta cctgccgcac
     1621 tgcgccagat atttgacgcc gaagatgtcc cgttgcaaca ggctgcggaa ctcccgcctt
     1681 cctatattaa cccgccgctt aaccggatta agattgttaa ccagcttaaa atctccggca
     1741 aagataatca gactcgtatt cctgacggca ttctctatat caacggtctg ccactggtcg
     1801 tctttgaatt taaaagcgcg gtgcgcgagc aggaagccaa tataggcgat gcctggaagc
     1861 agctctgtaa acgctaccgc cgggatattc cgcaactgtt tatctacaac gcgctctgca
     1921 ttattagcga tggcgtaaat aaccggatgg gcaacctgtt tgcaccctat gaatattttt
     1981 actcatggcg caaagtcacc ggtaatgaaa accgtgaaca ggacggaatt ccatcattgc
     2041 actcaatgat tcaggggctg tttcatccgg tacgtctgct ggatgtgatt aaaaacttta
     2101 tctgcttccc ggataaagcc aagcacgaag taaaaatttg ctgccgatat ccgcagtact
     2161 atgccgcccg caaactctat tacagcatca agcaagcgcg taaacctttc ggtagcggta
     2221 aaggcggtac ctactttggc gcaacgggct gcggtaaaag ctacaccatg caatttttaa
     2281 cccgcttgtt aatgaaaagc gtggagtttg ccagcccgac cattgttttg atcaccgacc
     2341 gcaccgatct ggacgatcag ctttctgcgc aaatgtgcaa cgccaaaaat tacattggcg
     2401 acgacaccat cgttcctgtt accagccgtg aagatttgcg taatcaactg gcgggacgca
     2461 atagtggcgg tgtattcctg acaacgatcc ataaattcac cgaagacacc gaactccttt
     2521 ctgaacgcag caatatcatt tgcatctcgg acgaagcaca tcgcagccag gttaacctcg
     2581 accagaaagt catcgtcgat aaagaaagcg gaaaagtgcg caaaacgtat ggctttgcga
     2641 aatacctgca cgattcactg ccaaatgcca cctatgttgg ctttaccggc acaccgattg
     2701 acgcgacgct cgatgtcttc ggtgaggtga tcgacagtta caccatgacc gaatccgttc
     2761 aggatgaaat cactgtacgc atcgtgtacg aaggccgtgc ggctaaagtg atcctggact
     2821 ccagcaaact ggaagaagtc gaaaagtatt acgaagagtg cgcaaacgca ggcaccaatg
     2881 agtggcaaat cgacgaaagc aagaaagcca cggctaccat gaatgcggtt ctgggtgatg
     2941 aagatcggtt aaaagccctc gcggaagatt ttgctaaaca ttatgaaaaa cgcgtagccg
     3001 aaggttccac cgtaataggc aaagccatgt ttgtttgtgc cagccgtgaa attgcctggg
     3061 atttctaccg ccagcttaaa gctattcgcc ctgcctggtt tgaagtgaag caagcacctg
     3121 atggcgtcgt cctgacggaa caggaacaaa aagagttacc gccttctgaa atggtgaaaa
     3181 tggtcatgac gcgcggtaaa gatgacgacg aggcgcttta tgatttactg ggcacgaaag
     3241 aatatcgtaa agagctggat aagcagttca aaaacgctaa atcgaatttc aaaattgcca
     3301 ttgtcgttga tatgtggctg accggttttg atgttcctga actggatacc atctatattg
     3361 ataagccctt acaaaaacat aaccttatcc agactatttc tcgcgttaac cgtaagctgg
     3421 aaggcaaaag caaagggtta gtggtggact acatcggcat taaaagtcag atgaaccagg
     3481 cactggcaat gtattcccgc attgatgcca ccaactttga agatattcag caatcggtaa
     3541 tagaagttaa aaaccatctc gatttgttgg ggcaagtctt ttacgaattt gacagtcggg
     3601 attattttag tggtgagcca caagcgcaat tatcctgcct caaccgcgcg gcggaatttg
     3661 ttctgcgtac ccagaaagtt gaacgtcgtt ttatgggact ggttaaacgc atgaaagccg
     3721 cctacgacgt ctgctgcggc agcgaagcat tatcacagac agaacgtgat catattcact
     3781 attatcttgc tgttcgttca attgttttca aactgacgaa aggtgacgca ccggatgtca
     3841 cccagatgaa tgcacgcgtt cgtgaaatga ttgcagaagc gctaaaagct gatggcgtag
     3901 aagaaattta ttttcttggc gataaaaaag cggaatccat cgatattttt gacgaagatt
     3961 atctggcgcg aattaacaag atcaaacttc cggcaacaaa gatccagtta ttacaaaaat
     4021 tactggaaaa agcgatcagc gacttcaaga aagtgaacca gttgcaaggg attaacttca
     4081 cccgccgctt ccaggcgatt atagatcgtt ataatgagcg gcgagaagat gatgtactca
     4141 acggtgaaga attcgataca ttcagtcagg aaatgaccga tattatctat gatattaaaa
     4201 ccgaaatggg cacctgggcc gatttaggca ttgatattga agaaaaagcc tttttcgaca
     4261 ttcttgctca tatgcgcgat aaatatcagt tcacctatga cgatgaaaaa atgctgtcac
     4321 tggcaaaaga gatgaaaagc gtggttgaca acacatcgaa atatcctgac tggagtaaac
     4381 gcgatgatat taaagcgaaa ctgaaagttg aacttattct gcttctacac aagcataagt
     4441 tcccgccagt agcgaatgat gatgtttata tgggtgtgct ggcacaggca gagaatttta
     4501 aagaacatca tatgagtgct ttaaattaat tatgaaggcc ggatacactt tgttatccgg
     4561 ccattctgaa ttaacgttaa tttctcttcc agatctttcc agtaacgctt ttattatttt
     4621 ctatttttag ctggactttc cctgtattta ctgatgatat atccaggtat ttagcgcggt
     4681 gcgaatgtgc gccaacacac ccgcaccgct catcacaatc cctatttcac gagaatagta
     4741 atcatgactg acacgcattc tattgcacaa ccgttcgaag cagaagtctc cccggcaaat
     4801 aaccgtcaat taaccgtgag ttatgcgagt cgctacccgg attacagccg tattcccgcc
     4861 atcaccctga aaggtcagtg gctggaaacc gccggttttg ccaccggcac ggcggtagat
     4921 gtcaaagtga tagaaggctg tattgtcctc accgcccaac cacccgccgc cgaagagagc
     4981 gaactgatgc agtcactgcg ccaggtgtgc aagctgtcgg cgcgtaaaca aaggcaggtg
     5041 caggatttta ttggggtgat tagtagcaaa acgccacgct aagtttctct ccccttcccc
     5101 atacgggaag ggaaattgcc ccctcactcc acatcctccc tgcgtaaccc gatatccctc
     5161 aaccgttcat cactcatctg ctgtacaccc gccgggcctg catttgccgc cgccaacgcc
     5221 tcaccgcctg tcagagttgc acaagtctga taaacggcgt tttggctttg ttttcgtgaa
     5281 attccatctg ctgcctcctc atctttcaga ggcttcatca tccgtcttct ggcagttcac
     5341 aatacagatg caaatattag ttttatttaa catacagaca ggttaaaaca gcatctgcat
     5401 ccggtttttt agcctcatct gtaccggttt ttctctctgt atggtccaaa taaggaatac
     5461 agcatgacgc gttatcaaca tctggcgact ctacttgccg agcggattga gcaagggctg
     5521 tatcgtcacg gggagaaatt gccgtcggtg cgcagcttaa gtcaggagca cggcgtcagt
     5581 attagcaccg tgcagcaggc gtaccagacg ctggagacga tgaagctcat cactccgcag
     5641 ccgcgttcgg gttattttgt cgcacaacgt aaagcccagc cgccagtacc gccgatgacg
     5701 cgtccggtgc agcgcccggt ggaaattacc cagtgggatc aagtgctgga tatgctggag
     5761 gcgcatagcg acagttccat tgttccgtta agtaaaagca cgccggatgt cgaaacgccc
     5821 agcctgaaac cgctgtggcg ggagctaagc cgggtggtgc agcataatct gcaaaccgtt
     5881 ctcggttatg acttgttagc cggtcagcga gtgttgcgag agcagattgc ccgcctgatg
     5941 ctcgacagcg gctcagtggt caccgctgat gacatcatta tcaccagcgg ctgccataac
     6001 tcgatgtcgc tggcgttaat ggcggtatgt aaaccgggcg atattgtcgc agtcgaatcc
     6061 ccctgttatt acggttcaat gcagatgctg cgcggtatgg gcgtgaaagt gattgaaatc
     6121 ccaaccgatc cagaaactgg catcagcgtt gaagcgctgg aactggcgct ggaacagtgg
     6181 ccgattaaag gcatcattct ggtgccaaac tgtaataatc cgctgggatt tattatgccg
     6241 gacgcacgca aacgggccgt tctctctctc gctcagcgtc atgatattgt gatttttgaa
     6301 gatgatgtct acggcgaact ggcgacggag tatccgcgcc cgcggaccat tcattcctgg
     6361 gatatcgacg ggcgagtgct gctgtgcagc tcgttcagta aaagtattgc accaggcctg
     6421 cgcgtgggtt gggtcgcacc ggggcgttat cacgataaac tgctgcatat gaaatatgcc
     6481 atcagcagct ttaatgtacc gtccacgcaa atggcggcgg caacgtttgt tctggaaggt
     6541 cactatcatc gccatatccg gcggatgcgg cagatctatc agcgcaattt ggcgctttat
     6601 acctgctgga tacgggaata ttttccctgc gaaatctgta ttacgcgccc gaaaggcgga
     6661 tttttactgt ggatagaatt gcctgaacag gtcgatatgg tttgcgttgc gcggcagctg
     6721 tgccgcatga aaatccaggt ggcggcaggc tcgattttct cagcttccgg caaataccgt
     6781 aattgtctgc gcatcaactg cgctttgccg ctcagcgaaa cctatcgcga agcactgaag
     6841 caaattggcg aggccgtgta tcaggcaatg gaatgaattc gaattagtgg cgaggcccct
     6901 gatttcctct cacgaatcgc ctgtgactcg ttttatcttt gcaggggcat cgccacgctc
     6961 cagcatggct taggcaaagt gtgctttgtt cataccggat gcggcgtgaa cgccttatcc
     7021 ggcttacaaa tcgcgcaaat tcaatagatt gcagaggtaa tgtaggtctg ataagcgtag
     7081 cacatcaggc agctttgcgt ttgtcatcag tctctgatta gtactcgatc catggatcgc
     7141 gcttgctttc aggcaagggt tgctctaatg cctgcatttg ctctgccgaa agtccagtat
     7201 atcgttgtat ccattcagga tccgcaccgt tctgcaaaag caatctaagg aggcgttgtt
     7261 ccccttcttc tttccccttt tccatcccta acagccatcc atcattatga attcgctctg
     7321 caatcgtcat tattcgctcc ctttgttgtg gcattcgacg ggtaagttca ctgataaact
     7381 cattaaaacg cgcttcatcg ccagtcagta aaatgtaatt taacagcgca gttatctggc
     7441 tgtcattagc acactctgta accagtaata ttaccagttg atcgataagc cccatcagat
     7501 cgcgctggcg aatatgcttt tggatcaact ccaacagggc gactctgcga tgctgcacaa
     7561 tctcgtcgtc tggcacgaca gtgacatcca ccagcgggaa cgctgcggta taaagcttcc
     7621 gtgcggtggt cgggtcagca aattcgtcca gccagcacag ggaccaggga taaggactac
     7681 ggctaccgtg ataaaacagc atcgggatga ccagcggtag cggtcggcgt ttatcatgct
     7741 cgatatggcg ctgcatcacc gccatggaat atcgcatcag gcgaaaggcc atatggatat
     7801 cctcgcggct ctgatgttca atcactacgt aaatataacc atcaccttca cgggtcttta
     7861 ccgaccacag aatatcggag tgtagcgccc gcaatttttc atcgacgaag ctggcggatt
     7921 ccagttttaa gctgtcgaga tcgcacagtt cacgtaaatc tttgggtaag tggatctcca
     7981 taaaatcccg cgcggtgtca gggtgcgtga gaaaggattt aaataatgcg tcatgcggcg
     8041 tactggtcgt gaagtttgtc atggcgatcc gtcacctgtt gataagatgg cccgacttta
     8101 gcgactaaca aaaccattct cacccggtaa tttcccattc cccgaggggc tttccaggct
     8161 atttttattc ttctgcaaac gatacatttt tccggaaaca ggctcgcatt ttacatttcc
     8221 tgtacccggc agatattccc ctttccaccg cactcttttg attaaccgca aattttaatt
     8281 acacttaagg tgtatatttt ctatgcaacc catcaattca agaggtgtaa tgtgctgatg
     8341 actatttgta atcgttatac attctgacct ggagtcagaa ggtatttctc tgtctgtgta
     8401 ttcacaggca gtgtggttga ttacatgaaa ttcagtacat ttatccttct cgcctgcctt
     8461 tcgtcattac cgacggtatt gaatttcgtt ttccccattg gggttctccg gacaaggagt
     8521 tgtttgttat gccacgtttt tttgcccgcc atgccgccac gctgtttttc ccgatggcgt
     8581 tgattttgta tgactttgct gcgtatctgt cgacggacct gatccagcca gggatcatta
     8641 atgtggtccg tgattttaat gccgatgtca gtctggcccc tgctgccgtc agtctctatc
     8701 ttgctggcgg tatggcgtta cagtggctgc tggggccgct ttccgacaga attggccgca
     8761 ggccggtgct gattaccggg gcgttaattt ttacccttgc ctgcgccgcg acaatgttca
     8821 ccacgtcaat gacacaattt cttatcgcgc gtgcaattca gggcaccagt atctgtttta
     8881 ttgccaccgt tggttatgtc acggtgcagg aggcgtttgg acagacaaaa gggatcaagt
     8941 tgatggcgat tatcacctcc atcgtactga ttgcgccgat tatcggcccg ctttccggcg
     9001 cagctctgat gcactttgtg cactggaaag tcctttttgc catcattgcg gttatgggtt
     9061 ttatctcatt tgttggctta ctgctggcga tgccagagac ggtgaagcgc ggcgcggttc
     9121 catttagcgc caaaagcgtc ttgcgcgatt ttcgtaatgt cttttgcaat cggctgttcc
     9181 tctttggcgc agcaaccatc tctttaagct atatcccgat gatgagttgg gtggctgtct
     9241 cgccggtgat cctgattgac gcaggcggct taacgacctc gcagttcgcc tgaacacagg
     9301 ttccggtgtt cggcgcggtg attgtcgcga atgccatcgt ggcgcgtttc gttaaagatc
     9361 cgaccgaacc gcgctttatc tggcgcgccg taccccttca actggttggc ctcgcgctgt
     9421 tgattatcgg caatctgctg tcaccgcacg tctggctgtg gtcggtgctg ggcaccagtc
     9481 tgtatgcttt cgggattggt ttgattttcc cgaccttatt ccgctttacg ctgttttcca
     9541 ataacttacc gaaagggacc gtctccgcat cgctgaatat ggtgatcctg atggtgatgt
     9601 ctgtctcggt cgaaatcggc cggtggctgt ggtttaacgg cggtcgcttg ccgtttcatc
     9661 tgttagccgt cgtggcgggc gttatcgtcg ttttcaccct ggcgggattg ctcaatcgcg
     9721 tgcgccaaca tcaggctgcc gagctggcgg aggagcggtg atttttgcgc gatcccgccg
     9781 tcaggctcta ttcttaacat tatgaataaa ctcattgaac tcagacgcgc caaaatgttg
     9841 gcgctctctt tactgcttat cgccgctgct acctttgtcg ttacgctgtt tttgccgccc
     9901 aatttttggg tgagcggcgt gaaggcgatt gctgaagcgg cgatggtcgg cgcgctggcg
     9961 gactggtttg cggtggtggc gctgtttcgc cgcgtgccga ttccgatcat ttctcgtcat
    10021 acggcgatta tcccgcgtaa taaagaccgg attggcgaaa atctcggtca gttcgtgcag
    10081 gaaaaatttc tcgataccca gtcgctggtg gcattgattc gacgtcacga accggcgttg
    10141 ttgattggca actggtttag tcagccggag aacgcccgcc gcgttggtca gcatctgttg
    10201 cagatcatga gcggttttct tgaactgacc gatgatgcgc gtattcagcg cctgcttaag
    10261 cgcgcggtcc accgggcgat tgataaggtt gatctttctg gcaccagtgc gttgatgctg
    10321 gagagtatga ccaaaaacga tcgtcatcag gtgctgctcg atacgctgat cgcacagttg
    10381 atcgcccttc tccagcgcga taaatcgcgc aagtttatcg cccagcaaat tgttcgctgg
    10441 ctggagagcg agcatccact gaaagccaaa attttgccca ctgaatggct gggcgaacat
    10501 agcgcggagt tggtttctga cgcggtgaat tctttgcttg atgatatcag ccgcgatcgt
    10561 gcgcatcaga tccgccatgc gtttgatcgc gccacctttg ccctgatcga caagctgaaa
    10621 aacgatcctg aaatggcagc gcgagccgat gccgtaaaaa gctatctgaa agaagacgaa
    10681 gcttttaacc gctatctcag tgaattgtgg ggggatttac gggaatggct gaaagtggat
    10741 atcaacagcg aagattctcg tgtgaaagaa cgtatcgcac gagcgggtca atggtttggc
    10801 gaaacgttaa ttgccgatga tgccttgcgg gcgtcgttaa atggtcatct cgaacaagcc
    10861 gcgcaccgcg tcgcgcctga gttttccgca ttcctgacgc gccacatcag cgatacggta
    10921 aaaagctggg atgcgcagga tatgtcgcgg caaatcgagt taaatatcgg caaagatctg
    10981 cagtttatcc gtgtcaacgg tacgctggtt ggcggttgta ttggacttat tttgtatttg
    11041 ctgtcgcagc tcccggcctt gttccccctc ggcaatttat agaaatccat gaaaaactaa
    11101 gtggctaacc gcgagagaga tcaaataaac cacactaaaa gaatggtaat aatgtggcct
    11161 cttttcatta tctcccgtgg tacggggaag gaaaatcatg tcacttgtca ccgatctacc
    11221 cgccattttc gatcagttct ctgaagctcg ccagaaaggc tttctcaccg tcatggatct
    11281 caaggagcgc ggcattccgc tggttggcac ctattgcacc tttatgccgc aagagatccc
    11341 gatggcagcc ggtgcggttg tggtttcgct atgttccacc tctgatgaaa ccattgaaga
    11401 agccgagaaa gatctgccgc gcaacctctg cccgctgatt aaaagcagct acggcttcgg
    11461 caaaaccgat aaatgcccct acttctactt ttctgatctg gtggtcggtg aaaccacctg
    11521 cgacggcaaa aagaaaatgt atgaatacat ggcggagttt aagcccgttc atgtgatgca
    11581 actgccaaac agcgttaagg acgatgcctc gcgtgcatta tggaaagccg agatgctgcg
    11641 cttgcaaaaa acggtagaag aacgttttgg gcacgagatt agcgaagatg ctctgcgcga
    11701 tgccattgcg ctgaaaaacc gcgaacgtcg cgcactggcc aacttttatc atttggggca
    11761 gttaaatcct ccggcgctta gcggcagcga cattctgaaa gtagtctacg gcgcaacctt
    11821 ccggttcgat aaagaggcgt tgatcaatga actggatgca atgaccgccc gcgttcgtca
    11881 gcagtgggaa gaaggccagc gactggaccc gcgcccacgt attttaatca ccggctgccc
    11941 aattggcggc gcagcagaga aagtggtgcg cgcgattgaa gagaatggcg gctgggttgt
    12001 cggttatgaa aactgcaccg gggcgaaagc caccgagcaa tgcgtggcgg aaacgggtga
    12061 tgtgtacgac gcgctggcgg ataaatatct ggcgattggc tgctcctgtg tttcgccgaa
    12121 cgatcagcgc ctgcaaatgc tcagccagat ggttgaagaa tatcaggtcg atggcgtagt
    12181 tgatgtgatt ttgcaggcgt gccataccta cgcggtggaa tcgctggcga ttaaacgtca
    12241 tgtgcgccag cagcacaaca ttccttatat cgctattgaa acagactact ccacctcgga
    12301 tgtcgggcag ctcagcaccc gtgtcgcggc ctttattgag atgctgtaag gagtggcagt
    12361 gacatattcg attggcattg attccggctc aaccgccacc aaagggatct tactggctga
    12421 cggcgtgatt acgcgccgtt tcctcgttcc aacatccttt cgcccggcaa cagcaattac
    12481 tgaagcctgg gaaactctgc gcgaagggtt agagacaacg ccgtttctga cgctcaccgg
    12541 ctacggacgg caactggtgg attttgccga taaacaggtg acggaaatct cctgtcacgg
    12601 gctgggcgca cggtttcttg cgccagcaac gcgcgcagta atcgacatcg gtggtcagga
    12661 cagcaaagtg attcagcttg atgatgacgg taacctgtgc gatttcctga tgaatgacaa
    12721 atgcgcggcg ggcaccgggc gtttcctgga ggtgatctcg cgcacgcttg gcaccagcgt
    12781 cgaacagctc gacagcatta ccgaaaatgt cacgccgcac gccatcacga gtatgtgcac
    12841 agtgtttgct gaatcagaag tgatcagcct gcgctctgcg ggcgtagcgc cagaagcgat
    12901 tctcgccgga gtgattaacg cgatggcgcg gcggagtgcc aatttcattg ctcgtctctc
    12961 ctgtgaagcg ccgattctgt ttactggtgg cgttagccac tgtcaaaggt ttacccacat
    13021 gctggaaagc catctgggaa tgccggtaca aactcaccct gatgcgcaat tcgctggtgc
    13081 aattggcgcg gcggtgatcg gtcagcggca gagaaaacgc gcatgaaaga gcatctgttt
    13141 ttatttcact ccacggtcgg cgtcatacaa acccgcaagg cgttacaggc agcgggcatg
    13201 acttttcgcg tcagcgatat tccccgtgat ttacgcggcg gctgcgggtt atgcatttgg
    13261 ctgacctgtc cgcctggcga ggaaattcaa tgggtgatcc ctgggcatac ggaatcagtt
    13321 tattgtcagc aggatggtgg ctggcgctgc attgcacatt acgggatttc tccgcgttaa
    13381 ggattcatta agatcgctac gtatacttct cgatatctta ttgatttgcc ttaaagggat
    13441 taccgtgaca aaaagtattt cattaagtaa acgcatcttc gttattgtga ttctttttgt
    13501 cattgtggca gtgtgtactt tcttcgtgca atcttgcgcc cgtaaaagca atcatgcagc
    13561 atcatttcag aattaccacg ccaccattga cggcaaagaa attgcaggca taacgaataa
    13621 tatctcttcg ctgacctggt cggcacaaag caatactctt tttagcacta tcaacaaacc
    13681 tgccgccatt gttgaaatga ccaccaatgg tgatttgatc cgcacaattc ctctggattt
    13741 tgttaaggat ctcgagacga tcgaatacat tggcgataat cagtttgtca ttagcgatga
    13801 gcgcgattac gccatttatg tgatttcact gaccccaaac tcggaagtaa aaatcctcaa
    13861 aaaaataaaa atcccgctgc aggaatcccc aaccaattgc ggttttgaag gattggccta
    13921 ttctcgccag gatcacactt tctggttttt taaagagaaa aacccgattg aggtctacaa
    13981 agttaacggg ctattaagta gcaatgaatt gcacatcagc aaagacaagg cactacaacg
    14041 gcaatttact ttagatgatg tgtccggcgc agaatttaac caacaaaaaa atacgcttct
    14101 ggtgctgtct catgaatctc gcgcactcca ggaagtaacg ctggttggcg aggttatcgg
    14161 tgaaatgtca ctaaccaaag gcagtcgagg tttgtcgcat aacatcaaac aggccgaagg
    14221 ggttgcgatg gatgcctctg gtaatattta tatcgttagc gaaccaaatc gtttttatcg
    14281 ctttacccct caatcatcgc attaacaggc tggcatgctt cacctttttg ttattaactt
    14341 gttaacaaca aaaatgacaa acaaggttaa acatgccttc gtccacgcat cccgtagaac
    14401 gcttttcttt cagcaccgcg cttttcggga tgctggttct gaccttaggt atgggtttag
    14461 gccgcttcct ttatacgcct atgttgcccg tcatgatggc ggaaggatcg ttttcattta
    14521 gccagctctc gtggattgcc agcggcaact atgcggggta tctggctggc agtctgctat
    14581 tttcttttgg cgcatttcac cagccatcgc gcctgcgccc atttctgttg gcttctgccc
    14641 tggcgagcgg gttgttgatc ctcgcaatgg catggttgcc gccatttatt ctggtgttat
    14701 tgattcgcgt cctggcgggt gtcgccagcg ccggaatgct gatttttggt tcgacgctga
    14761 ttatgcagca cacgcgccat ccttttgtgc tggcagcttt gttttctggc gttggcattg
    14821 gcatcgcact gggcaatgaa tatgttctgg caggcctgca ttttgacctc tcctcgcaaa
    14881 cgttatggca aggcgccggg gcgctttctg gcatgatact gattgcactt acgcttttaa
    14941 tgccctcgaa aaaacacgcc atcgtaccaa tgccattggc aaaaacggag caacagataa
    15001 tgagctggtg gttactggct attctgtacg gcctggcggg atttggttat atcatcgtcg
    15061 cgacttacct gccgctcatg gcgaaagatg caggctcacc attgttaacc gcccatctct
    15121 ggacgttagt cggcttatca attgtgcctg gttgctttgg ctggctatgg gccgcaaaac
    15181 gttggggggc tttgccctgc ctgacggcga atttgctggt gcaggctatc tgtgtgctgc
    15241 ttactctcgc cagcgactcg cctctcttgc ttatcatcag cagtcttggt tttggcggta
    15301 cctttatggg cacgacatct ctggtgatga ctatcgcccg ccaactgagc gtaccgggaa
    15361 atctcaatct tttaggcttt gtgacgctca tttacggcat tgggcaaatc ctcggcccgg
    15421 cgctgaccag tatgctcggc aatggaacat cggcgcttgc cagcgccact ctctgcggcg
    15481 cggcggcgtt gtttattgct gcgcttatca gcacagtgca attatttaag ctacaagtgg
    15541 tcacttcgtg agagaagttt cccttaccac tcaagaaact caaccgcaac tgttgatgtc
    15601 agccagaccc cgttttccgc ttgccaaaaa gggatgcctc gtttcgccat ttcctgtgct
    15661 ttgacggtta aaataactgg cgatccgtgt cttgctccca ctttgcgtgc cgttgcttca
    15721 tcagcagaga gatgcacgta atggcgctcc cctgcaatca gcccttgttt ttttatctcg
    15781 tcaagaaaac ggcttgccgt accatggtag agaaattgtg gcggtgtttt ttcgatgaag
    15841 ccaatggcga cctgcgaagt cgaatgcccc tgaacggcgc gaatacacgt accatcactg
    15901 gaataactaa agcgcttttt atcgctggta gcgacaacga tatccagtaa tgcgcgggtt
    15961 aaccgcttgc ccgctttttg ggcacagagt atgagcttat cgatatccgc ccatccttca
    16021 cggtccagca cgatgccaat cgcctctggt ttatgtcgga ggacaaaact taaaaacttg
    16081 ctcgtctctg ccagctcttt gtcgttgtat tttgccatct cgttctcgtc cctggatttt
    16141 ctgtttttat ttactgatgt cattgtcact gggtttatga attttctgca actcaatcct
    16201 gcgcagaacc tttgccacac agcgtcattt ttccgctatg caaaaaacgc atagccagaa
    16261 acatacttgc attttcccca tacccacatg gctgataagg tttttcagct ccaggaattt
    16321 tcctggctca agcaacacac aaacacaaaa aaacaatggg gattgttatg acacaacaag
    16381 gggatgctgt agccggggag ctggcgacag aaaaagtggg cattaaaggg tatctggctt
    16441 tttttctcac cataatcttt ttttccggtg ttttttccgg gacagacagt tggtggcgcg
    16501 tttttgattt ttccgtgctg aacggatcgt ttgggcaact gcctggtgcg aatggcgcca
    16561 ccacctcgtt tcgcggcgca ggtggcacag gagctaaaga tggctttctc ttcgcgctgg
    16621 aactggcacc gtcagtcatt ctctctctgg ggattatttc aattaccgat ggtcttggcg
    16681 gattacgcgc cgcgcagcag ttaatgacgc ccgtgctaaa accgttgttg ggcattccag
    16741 gcatctgttc actggcgctg attgctaacc tgcaaaacac cgatgccgcc gcaggtatga
    16801 ccaaagaact ggcgcaggaa ggggaaatta ccgaacgtga taaagtgata ttcgccgcct
    16861 atcaaaccag cggtagcgcc atcatcacca actacttctc ttccggcgtc gcggtgtttg
    16921 cctttctggg cacgtccgta atcgtacctt tagccgtcat cctggtgttt aaatttgtcg
    16981 gagccaacat tttgcgcgtc tggcttaact tcgaagaacg tcgcaaccca acgcaaggag
    17041 cgcaagcatg acaactcagg tccgcaaaaa cgtcatggat atgtttattg atggcgcgcg
    17101 ccgtggcttt actatcgcta ccaccaacct gctgccaaac gtggtgatgg cctttgtcat
    17161 catccaggcg ctgaaaatta ctggcctgct cgactgggtg ggacatatct gtgaacccgt
    17221 aatggcgctg tggggattgc ccggtgaagc ggcaaccgtc cttttagccg cattgatgag
    17281 catgggcggc gcagtcggtg ttgccgccag tctggcaacc gctggcgcat taaccggcca
    17341 cgatgtcacc gttttactgc ccgcgatgta tttaatgggc aacccggtgc aaaatgtcgg
    17401 tcgctgcctg ggaacagccg aagtgaatgc caaatattac ccgcatatca ttacggtctg
    17461 cgtgattaac gcattgctgt cgatctgggt tatgcagctt attgtttaac aaggagttac
    17521 catgattgat tattccgcag ccggttttac cctgctgcag ggagcgcatt tgtatgcgcc
    17581 ggaagatcgg ggaatttgcg atgtcctcat cgctaacggc aaaattattg ccgttgccag
    17641 caacatccct tctgacattg taccagactg cacggttgtc gatctcagcg ggcagatcct
    17701 ctgcccaggt tttattgatc aacacgtcca tttgattggt ggtggcggcg aagcaggtcc
    17761 cacgacacgc acgccggaag tggcgttaag tcgtctgacg gaagcgggcg tcacgtcagt
    17821 ggttggtctg ctgggtaccg actctatctc tcgccacccg gaatccctgc tcgccaagac
    17881 ccgtgcgctc aatgaagaag gcatcagcgc ctggatgctg accggcgctt atcatgtccc
    17941 ttcccgcacc attacaggtt ccgtggaaaa agacgtggcg attatcgatc gcgtgattgg
    18001 tgtgaaatgc gccatctctg atcaccgttc tgccgcacct gacgtttatc acctggctaa
    18061 tatggcggcg gaatcccgcg ttggcggttt gcttggcggt aaacctggcg tcaccgtgtt
    18121 ccacatgggc gacagcaaaa aggcgttaca gcctgtctat gacctgctgg aaaactgcga
    18181 tgtgccgatc agcaagctgc tgccgaccca cgttaaccgc aacgttccgt tgtttgagca
    18241 ggcgctggag ttcgcgcgca aaggcggcac catcgacatc accagtagca ttgacgaacc
    18301 ggtcgcccct gccgaaggta ttgcccgcgc cgttcaggcg ggtattccgc tggcacgcgt
    18361 caccctcagc tccgacggca acggtagcca gccgttcttt gatgacgaag gaaatttaac
    18421 ccatatcggt gttgcaggtt ttgaaacatt gctggaaacc gtgcaaatgt tggtcaaaga
    18481 ctatagcttc agccttagcg atgccctgcg cccgctcacc agtagcgtag ccggtttcct
    18541 taacctgacc gggaaaggcg aaattctgcc aggcaatgac gcagacttac tggtcatgac
    18601 gcctgaattg cgcattgagc aggtatacgc tcgcggcaaa ctgatggtca aagacggcaa
    18661 agcctgcgtg aaaggaacgt ttgaaacggc ataaccggtt gtcagccgcc cgaatggacg
    18721 tagaatgccc gcaagggcgg ctgacagagt ataacgtaat ggatgactgt ggtgcggttt
    18781 tgcataacat tgaaaccaaa tggctttatg attttctgac cctggaaaaa tgtcgcaatt
    18841 tttcccaggc ggcagtcagt cgcaacgtct cgcaaccggc attcagccgc cgcatccgtg
    18901 cactggaaca ggcgattggc gttgaattgt ttaaccgcca ggtgacgccg ctgcaactct
    18961 cggaacaagg gaaaatcttc cattcgcaga tccgccatct gttgcaacag ttagaaagca
    19021 acctggcaga gctgcgtggc ggcagcgatt acgcgcaacg taaaatcaag atagccgctg
    19081 cacactctct ttccctcggt ctgttaccgt ccattatcag ccagatgccg ccgctcttta
    19141 cctgggcaat tgaagccatt gatgtcgatg aagcggtcga taaactgcgc gaagggcaaa
    19201 gtgactgtat tttttccttt cacgacgaag atttgctgga agcgccgttt gaccacattc
    19261 gcttatttga atctcaatta ttccccgtct gcgccagtga cgaacacgga gaagcactgt
    19321 ttaacctcgc gcagccacac tttccgttac tgaattacag ccgcaactcc tacatggggc
    19381 gattgattaa tcgcaccctg acgcgccaca gtgagttgag tttcagcacc ttttttgtct
    19441 cttcgatgag cgagctatta aagcaggttg ccctcgacgg ctgtgggatt gcctggctgc
    19501 cggaatacgc tatccaacaa gaaattcgca gcgggcagct cgttgtactg aatcgggaag
    19561 aactggtgat cccgattcag gcctacgcat accggatgaa tacccgcatg aatccaattg
    19621 ccgaacgctt ctggcgtgaa ctgcgcgagc tggagattga acctggttga aagttttctg
    19681 cagaaaatct tccggcgctg ggaattatga ttgaggttcc tgccgcagtc atgattgcag
    19741 acaaattagc cagtgaagtc gatttctttt ccatcggcac caacgatatg acgcaatata
    19801 ttatggcggc agacagagga aatagcaccg tcgcgaaact ggtcgattat tgtaatgacg
    19861 cggtgattaa cgctattgct atggtgtgtc aggccggacg taataatgaa attccggtca
    19921 gtatgtgcgg cgaaatggct ggagatatcc agcaaacagc acgactgctc accatgggga
    19981 ttgataagct cagtgccagt ccatcccggc ttccggcctt gaaagcagca atcagagcct
    20041 cccactaatt gtgacctaac gcgatacgtc ttgcgcgtat cgcgtttccc gtcatttact
    20101 gcggcatcgt gcgatgccag ttccccttct gccagaccag gtcaaacgtt agcggctcat
    20161 catcgtggta cagcatcatc tccagactaa tggccaactc gtggcaatag gcttctttca
    20221 gattgacctg caatgccgca atacgtggct catgaaataa aagcgcggaa cgtaactggc
    20281 ggcaatacca actctggaga agcttgtcgc tctgattatg cccttgccac tcaggcatgc
    20341 ctgaaacaag taatattggt agcggtgaat ggggagaact aaaaagttga aagagatccc
    20401 ggcatacgga atcacgcagc gataacgtat tgtgtcctgt actttcaggt aataccgtct
    20461 gaactgattg gcgcatcatt ttgcgcactc ctgacgttta gtggcatctg tatcctgccc
    20521 tggctgccgg acaccactgg gttgatgctc atattccata atcattcctt tatgctcatt
    20581 gcgtccctgg cactttcata agggatttat aagatccgcc aaagataaaa cactgtacag
    20641 aaaagaatat ccactgactt tttatttaaa aaggtatcat ttgacaaaag tcattaaaca
    20701 taacggaaat acgttaacat attctgatta ttccgaataa tttctcagcg ttaaactgat
    20761 atataattat tccaaaacaa tatatcgtac gtaatgatta atttacgcat aatatattct
    20821 gcagaacata attaactatt cagggaacat acatagtgca aagcggttca actttgcacc
    20881 agaaatgagg gatcaggcgt cgactttatc cagcggccat gaatcaaaca gatagccgtc
    20941 aaaataaata tcgtcaacgt tcgagaattc cagcagacgt tgtttaacgt tttccagatg
    21001 ctgccacatt gccagcttcg ccgcacgggc atcttttttg attaacgcgg cgaggatctg
    21061 tttgtgatcg cccaaccact ctttgcgata caggctgtca tcgagatggc tgtgcaattg
    21121 gatccacatt ggattgtttt cccgccactg ccaggactga cggaataact ccaccagcat
    21181 gctgttatgc gttgcttcgg caatggcgag atggaactgc atatcaccgc tttcgctgct
    21241 acccggcgcg ctggaagcca gttcacgctc ttccagttgc aatgcctgac gcattttgac
    21301 gatatcttcg cgggtagcct gcaaagcggc aaattccgcg atgttacttt ccagtaactg
    21361 acgcgcctgt aacagctcaa aaggaccggc atcattacag acgttggcat ccggactgtc
    21421 tgtgttgtgg ctgcctgagc tatcaagaac ataaataccg gcaccccggc gcacttccac
    21481 cagccctttg atctccagca tgatcagcgc ttcacgtacc accgtccgcg tgacatcaag
    21541 catttctgca atttcacgct ccggtggcag ccgttcgcca ggattgtacg gcgtctttat
    21601 gatcagatcg cggatcatcg ccccgacttc ctggtaaggt ctttgcgcag aggtggcaga
    21661 tttcatggtg ttgtgtccgt taggtgcggt taatcaaatt ttttccggat gcggcgtgaa
    21721 cgccttatcc gacctacaac gggcaatttt acggcacccg ttaagctcaa actataccat
    21781 tagcactgga tgttggcacg tacaggtggg caaaaaaacg aaaactggtc aaccaaatat
    21841 tagtttgatt gaccagtaag tctgtagggt aatcagttag ttacagcgca gccacacact
    21901 cgcgcgcacc gcgctcacac agctgctgat atgccgccgt caccgcgcca acaaagtcgg
    21961 cgttctgcgg cagatcatcg gcaaagatac cgctcaggcc cagcagtgct ttcacgcggt
    22021 ctgcaccctg atactgcgcg ttgatcttct ggaactccgc cagcatcggg tcaaccacgt
    22081 caatggcatt accctgctca tccacgccct gggtgtaacg catccagccc gccacgccca
    22141 gcgccaggtg acgccagcta ccgccgtttt gcaggtgcag acgcaccggg tccagcagac
    22201 gctgcggtaa cttctggctg ccgtccattg caatctgcca ggtacggtga cgcagagacg
    22261 ggttgctgaa acgctcgatc agcagcgtcg cataagcgtt caggtctgta ccttccggca
    22321 tcgacagcgt tggcgcttgt tcctgcatca tcagggcaaa ggccgctttg cgataagccg
    22381 ggttagtcat ggtgtcggca atagtttcat agccgccgag gtaaccgagg tacgccagga
    22441 aagagtggct accgttcagc atacgcagct tcatcatttc gaacggtaca acatccgcaa
    22501 caaactgcgc gccgacttta tcccagtccg gacgaccatt aacgaagtta tcttcaatta
    22561 cccactgacg gaacggttcg caggcaatgg cgcacgggtc gtaaacaccc agctggtcag
    22621 caatttcctg taaggtttct ggcgtcgccg ccggaacgat gcggtcaacc atggtgcacg
    22681 ggaaggtgac gttctcttca atccatgccg ccagttgcgg gtcacgcgcc tgagccagcc
    22741 caagaacggc aaccttcgcc acatgaccgt tttcacgcac gttatcgcag gacatcaccg
    22801 taaatgcttt cagccctttt tcacgacgca gacgcagggc ttctacgatg taaccaatcg
    22861 cagacttcgg cgcagtcggg ttttccagat cgtgcttgat cagcgggtta ttgagatcca
    22921 gttgaccgct tgccgcatct gcgcagtagc ctttttccgt gaccgtcaga gagacaatcg
    22981 ccgtctgcgg acgcgccatc gcgttgagaa taccttcgca accgtcgatt tccgggtgca
    23041 gggcttcttt catcgaaccg ataattttca gctcggtgct ttctgcgcct ttttccgcca
    23101 cggtgtacag cagttgctgt tttttcaggt tttcgatcag cacgcggtcg ttgcctggca
    23161 tcaggttaac ttcgcagatc ccccagtcgc tatccgtgct ttccagcagg tgatgagtgt
    23221 acagcgcctg gtgcgcgcgg tgaaacgccc cgcaaccgag atgcacaatg cgtgattcca
    23281 gacgagaatg atcccatgac gggcgggcaa ccggcagatt gctgtcaaca atagtagtca
    23341 tcgtaaactc cagaatccgt cttcagagag tgacggcaag gaagaaggaa ccggcacggc
    23401 gcgcagccat gccggtggat attaacggct aaagaaagcg cgctggatcg ccagttcgac
    23461 accgcgaact tcggccaggc ctttcagacg accaattgcg gagtaaccag gattggtttt
    23521 cttcttcagg tcgtccagca tctgatgacc gtggtccgga cgcatcggga tcaggtcttc
    23581 tttgccttcc gctttacgac ggtgttcttc ttcaacaatc gctttcacca cttcgtacat
    23641 atcaacgtca ccgttcaggt gcgccgcttc gtggaaggtt ttcgggttat cttcacgcat
    23701 ggtggagcgc agatgggtga agtaaatacg cggaccgaac tgcttgatca tatcaaccag
    23761 atcgttgtca gcacgcacgc cgtaggaacc ggtgcacatg gtgaaaccgt tcgccatgct
    23821 gtttacggta tcaaccatcc actgcatatc ttcaatggtg gaaacaatgc gcggcaggcc
    23881 gaggatcggg cgcggcggat cgtccgggtg aaccgccata cgcacgccaa cttcttcagc
    23941 aactggaata atcgctttca ggaagacagc gaagttttcg cgcagcttcg ctttgtcgat
    24001 atctttgtac agctccaggt gcttacggaa ctggtcgagg gtataccctt cttccgcacc
    24061 tggcagaccg acaatgatgt tacgggtcag acgcgctttg tcttcgtcgc tcatagtggc
    24121 gaagcgaaca gctgcctgag caatttcttc ttcggtgtaa tccgcttccg cgcctggacg
    24181 cttcaggata tgcatttcga atgcagcgaa ttcgatctgg tcgaagcgca gagctttgga
    24241 gccgtctggc agcacgtatt cgaggtcagt acgggtccag tcgagcaccg gcatgaagtt
    24301 gtagcacacg gtgcgaatgc cgcactgcgc caggttgcgc agggtctgct gatagttagc
    24361 aatccactgc tcatagttgc cagtgtgggt tttgatatct tcgtgaattg gtacgctttc
    24421 aacgacagac cacaccaggc ctgcgtcttc aacgatcgcc ttgcgtttga ggatctcttc
    24481 tacggaccat acttcgccgt tcgggatatg gtgcagcgcg gtaaccacgc cagttgcgcc
    24541 cgcctgacgg acatcagcta aagaaaccgg atcgtttggg ccgtaccagc gccaggtctg
    24601 ttccatgatt tttcctcttt cacatagtgg gttccacagc aaagagacgt ggagccccgg
    24661 agttgtttag gtatccggat gaacaaccag accgcagccg tggcgttgca tcggatgaga
    24721 tggcgtataa gttctacctt ttggtaagac atgtcaaccc gaaatctgaa attggttaac
    24781 cacatcacaa gaatttcact ctcctggttg ttgctgcaaa ttgcagcaaa gggtaatcga
    24841 tggttattga tcagaggatg tgacattcat cgcaacaatg gttgaccaat ttacataaca
    24901 tgtcgcccaa aataacaacg gttcaaccag gtaaggtaaa acgttatgca tgtgcttaac
    24961 attctctggg tggtattcgg cattggtctg atgctggtac tgaatttgaa gttcaaaatc
    25021 aattcaatgg tggctttgtt ggtggcggcg ctgtccgtcg ggatgctggc gggcatggat
    25081 ttgatgtcgc tgctgcacac catgaaagcg ggcttcggca acacgctggg ggaactggct
    25141 atcatcgtgg tgttcggtgc ggtcatcggt aaattgatgg tcgactccgg cgcggctcac
    25201 cagatagcac atacgctgct ggcgcgtctc ggtctgcgct atgtacagct gtcggtgatt
    25261 atcatcggcc tgattttcgg tctggcgatg ttttatgaag tggcctttat catgttagcg
    25321 ccgctggtta ttgttattgc cgccgaagct aaaattccgt tcctgaaact ggcgatcccg
    25381 gcagtagcag ctgccactac cgcacattca ctgttcccac cgcagccggg tccggtggca
    25441 ctggtgaatg cttatggcgc ggatatgggg atggtttata tctatggcgt actggtgacg
    25501 atcccaagtg tcatctgcgc aggtctgatc ctgccgaagt tcctcggcaa tcttgagcgc
    25561 ccaacgccat cattcctgaa agcagatcaa ccggtagata tgaataatct gccctctttc
    25621 ggcgtttcga ttctggtgcc gctgatcccg gcgatcatta tgatctctac caccatcgcc
    25681 aatatctggc tggtaaaaga tacccctgcc tgggaagtgg ttaactttat cggttcctcg
    25741 ccgattgcaa tgtttattgc gatggtggtt gcattcgtac tctttggcac cgcgcgtggt
    25801 catgacatgc agtgggtgat gaacgctttt gaaagcgcgg tgaaaagcat tgcaatggtg
    25861 attctgatca tcggtgcggg tggcgtgctg aagcagacca tcatcgacac cggcattggc
    25921 gacaccatcg gcatgttgat gtcccacggc aatatctcgc cctacatcat ggcatggctg
    25981 atcaccgtac tgattcgtct ggcgacgggt cagggtgtcg tttcggcgat gaccgctgcc
    26041 gggattatca gtgctgcaat ccttgatcca gcaaccggtc agctggttgg cgtgaatccg
    26101 gcgctgctgg tgctggcgac ggctgcgggt tccaacaccc tcacgcacat taacgatgca
    26161 tctttctggt tgttcaaagg ttactttgac ctgtcagtaa aagacacgtt gaaaacctgg
    26221 ggtctgctgg agctggtcaa ctccgtggtt gggctgatta ttgtgttgat tattagcatg
    26281 gtagcgtaaa agaacaccgg agcctgccaa tggcaggctc tgttcggatt gtcggtaaag
    26341 tgcgctttgt ttatgccaga tgcgacgctg acgcgtctta tctggcatac aaagggctaa
    26401 cgtgcaggtt ttagcttcag gtaatattgc gtacctgcat tagcaatgcc ctgtgatttc
    26461 tttattgata aacaaaagta acgccaataa tcgattgcac attccctgca gtcacctgcc
    26521 ctccggtacg tgcgtaattt gccgttaatc ccagacttac cgccgaagtc cccactgctc
    26581 ctaacgatac cgtgttattc gctggaataa tcgtaccgtt gcgcgtcaac tgtacgccga
    26641 cgccctgtgc tggtgaaaac gaggcggtat tggtgaaaat cgagttgccc gcatctgcgg
    26701 ttgtgccgga gaggtaatac cccaggtttt ggcttttcgc acaataaacg gtaagaggaa
    26761 tcggcaccga accgggatag tccggcagag taacggtgac atcacgagca gaaacatcac
    26821 agccgccagt aggcaccacc acatcattat tggcgtaaat attccacaca aactggaaat
    26881 catcgctgtt atagttgttg gtctgtcgca aaattagcac ggcaattaat gagccagctt
    26941 taatcgccac cccgcccgca ctgctcacag gcgtcaaata aagcgccacc ggccacggct
    27001 tatccgttct cgaattataa acaacgcgcg gcgtttcgct agtggtcgga aatggatagc
    27061 tactgccact atattttacg gtcccggaaa aattagataa cacgccgcca taagccgagc
    27121 ctcgttgcag tgtgacatag tctgtaatgg tttccggata atcgttatgg caaaagattt
    27181 gcgtcgaaag atccacgacc aggttttgcc ccacattcac ggcaggcgca aggtttacat
    27241 aaacattagc gctgccaccg ccaataggga tagcggtgcc attggcggtt ttacaggcga
    27301 atgaccaggc atttaccgac cagcccatca gcagtacagc aaacaggtaa ataactcgtt
    27361 tcattacaat catctcttcg gcttcagctg taggtatagg tgatgctaat tactgcctga
    27421 atggttccct gagtggctcc gccatttacc gtcaatgctc tgacctgtaa cgggaagtgc
    27481 gctgattgtg aggaatcatc cacctgaact gttttggttg cgccactatt caatgtgttg
    27541 ccactgtcat cctgtagctc taactggatg ttttgcgcgg tcccctggtt tttataatat
    27601 ccggtactgt cggctgcccc gctgaagccg gcggtgaccc tcgacgttcc caccggacaa
    27661 ttagtcaact caagcgcaac atcatgccag gccgatgccg ccccggcaga catcagactg
    27721 aaagaataaa gatcgccgag atctaccgtg gcattggtgg tggaaaccgt gcacggtttg
    27781 gcgacgacct taccgttcac cgtgatggtg acatcggctg cctgtatcgt cgcacttgcg
    27841 agcgccaaca ttgccgccaa taaataccca cgtttgcgcc atttcatgag catctccagt
    27901 tactgatatt caagagtgaa ggtagccgtg gcattgatat gccccgcagt gacaggcacc
    27961 tgtgtcgcca ttagccgggc gtaaaaattc agcgtatttg gtttacccgg cgtcagggtc
    28021 gtccacgaaa tcgcggacga tggggcatta aggggtatct gattttgttg ctcattcaga
    28081 agctgtattc ccagtcccgc agccgctgac accgtatttt caagtgcaag caggttggca
    28141 ttgtggctat ctgcaacacc ggtaaaccca acttttacgg cagaaacggc attaccacag
    28201 ggtgacagca aaatacgaaa tggaacgaca ggagtcgtcg cgccaatgtt gttaaattgc
    28261 ttcaccgcgt tttccatcag atcaacagta aaattggttg attcagcggc cacactacag
    28321 ccattatctc tgacatagcc gcggatagta atcgtgctat ccgcagccaa agcgtgactt
    28381 accgccagcc acaaaaaagc gcaaagaaga taaaaaggtt tgtttctcat cacgcctcct
    28441 taacgacatt cagctgatag ctgggttaat aactgctgct gactctctgg tggcagttga
    28501 taattggcga cacagtgagc attttcctct tctccccatt tcacctgaac ttttcccgct
    28561 aaaggcattc cgctgaggta aacctgacca ttatccgcaa caatgccgct actctggcta
    28621 ctctctgatg tcaccatcgc cccaaacggc agcggcttat tattgtgggt tagcgtcatg
    28681 agcagtttta tcccaacgcg cgctttaaac tctgctcgca cgatcgcccc acgggtggga
    28741 acaacgttag cgaccgcgtt atctaaatcg acgttatcag ccagggtatt ggtatccagc
    28801 gctactctgt tttcccgata ttcagtggca taaggcagca cggcataacc gcgccagtcg
    28861 gtacgcaccc ccgtctggtt ttcgactttt gcatcttttg cgccaggcgc tttaacaagc
    28921 accaccgtat cgtttaacgg ctgccccagc gttacgccat tggcatgagc cagtacccca
    28981 ccgctgactc cgtaatagag ctgcttaata tcatcgctat ggctgtaacc gatgttggca
    29041 ttgccgtaac caccgcgata attcagcgtg gcgtagcctg tacttccgct attaccatca
    29101 cctcccccgg cgtaaccggt ttgcacgcta tagctgaggt tgttgtcttc cagcaacgta
    29161 ccgtatacac cggccagatt ggtcatccga ccgttgagat cgtgtgacat gctgtaactg
    29221 gcactggcat gtcgccactg agatttactg tcagaacgca gccagtggct gaaaggaata
    29281 ttgacgttaa gcgctaacat ctgatcccgt cctttttgcc aggcgttttt cgtcaggcta
    29341 tagctgagcg tccagttgat atcttcgaat gcagtattta aaccagcctg gaattgctca
    29401 tcgacattac tcgttcccca ataagtttga tggctaccac tcaaatacag tgttgatgag
    29461 cgcccgagtt gctgagtaac ggtgagctgt aatttcccgc gtttgttata agcgagattg
    29521 taatagtcgg tgaatttcgg cttaacctga ataactccat cctgtgtttc gatgttgtag
    29581 ccattcattc gactgtatgt tgtatcagcg aaattaaaat acccactggt cgaataacgg
    29641 taacccacta actgaatatt cgtgcctgac tcattgagcg atttgttata aagaaaacgc
    29701 accgattgtc cgtcatgctg actgtcatcg ggaagtgtgg aattagcctg cgtcatatcc
    29761 acagacagag cgcccagtgc ccccatattt ttcccgatac cgaaattaaa cgcacgataa
    29821 cgatccgcca gttgcgttcc accatatatt gtccagccag ccggaaggcc gtggagtaat
    29881 gtactctgga aaaagcgggg tttttcctgt tgcgcatttc cactacggta ttctcctgcc
    29941 gtaatggaat aacgtgtatg cccttcacgc tgcaaaagcg ggactgacga atagggtacg
    30001 gtaaaaatct gcgtactgcc gtcagcctct ttaatcgtta cctgcaagtc accactatta
    30061 cctgcggcat agatatcgtt gatggtaaaa ggccccggtg gcaccgtact attataaatg
    30121 tcatacccat tttgtttaat agtgacctgt gcagtaccac gagcaatacc gtggatcacc
    30181 ggggcaaatc ctctttggct atcgggtaac atattgtcat ctgaggccaa ttgtgcgccg
    30241 cgaaagttaa taccatcgaa aatatcgccc tgagtataac catcacccag cgtcagccgg
    30301 gaacgtaacg gaattatgtc tcgctcaagc caggtattga tatgctgcca tttatttttg
    30361 ctacctgatg aactgtcgct actgttataa ctccaggtgg tattgtcgcg taaacgccac
    30421 gcaccaatat ttaacccact ctgtaggttt aaatatgcat aatggctgtt acccccaatt
    30481 cgattctgta cactatttcc gctgaaatta taattgagca atccggcatt aataccggga
    30541 tcccataact caggaggaat ataaccacgc gcgcgattac tcataaatgc ctgagggatc
    30601 gtcaggttga gtcgctgctg accaacatct aaatgcgcag tagcgtcatg gatcattgag
    30661 gttaatggca cgcaggcatc atccgccagc agattcatac cggagacaga agccgtattc
    30721 agccccatac tggcaagttg cgcgcgtgtc aggcagggaa caatcccttg ttcactgtcg
    30781 cccgtgttaa atgtgacatc acgcgttgcc atataaccat tattcaaata gatatcgacg
    30841 cgatacgtcc ctggcggtaa ttcttgccca ttttcaaaac gcgataaatc ggccacagcc
    30901 tggggatcat ccgctaaaaa gcgcggatta aaatagagtt cggcagatga caaaggtgcc
    30961 tgtgcggcaa aagcacaggc gacaaagagc cggacaaaaa aaccagccaa acgatgctta
    31021 cgaatatgca agcattgtgt gtttcgctgg taaagtctta aattcagata tgacattatt
    31081 cctccggttt aaaatcaccc ggcagtcaat tctttttatt caggcgaaaa attccccctg
    31141 cgttattcca ttacgcccgt cattttgggg gtaagtgcgc cataatcatt tattgttcgg
    31201 taagtaatat tgcttcctgc atcagaaggc aatttaaccg tgctttcgcc cattggaggc
    31261 accaatgcat tttcaagaac ccgggttccg gcattcaact ctgttaccgt caggtaatag
    31321 ggtgtcgggt taatcagcgt cagagaattc gcgctacgac gaaatcttaa tttttctgcg
    31381 gcctgatcgg gtggcaacgc taatttagcc gggcgatagt acagcttaat gcggctgata
    31441 attgcgagct gtagcgtatt ctcagtcaat tttgatttat ccattgacgg aatcgcttta
    31501 acgttcatcc agaataaact ttcccgatcc tgtggcaatt ggttatttgt tgcatcaaga
    31561 atacgtaagg tattctctct ttttcccttc atcgcaaaca gaggtggcgt cacgataaaa
    31621 cgaccatcct ttacaccatc ggcattttcc acccatgatt gaattaaata ggtgctattt
    31681 tcatcattat ttgtcacggc aagttgcact tgtttttgcc ctgccggata aattacgcgg
    31741 gtcgcaccta aggccactcc cgcttcagcg cgtccggcaa ccatcattgc catgaacatc
    31801 aggatacctg ccagcaagca gaatgttatt tcctgcgatt tccttacatt gacgttttta
    31861 ttactcactg tcctgttcct gttatcacat tatctgctga acaattactg ataggttaaa
    31921 gagaaccagg cctgggcatt ggcgatgccg ccagtaaccc gacgcccggt agcacgatat
    31981 ttggcgatga aatgtagcga agtagagcct gagtaaagcc gtttccagtt tgctggagga
    32041 cgattaatcg gtacgaggtt tccttcatca tcaaataacg ctacgccaat attggtggct
    32101 atccctggcc cctctcccac ggaaagcaca tccggatttt taccatccgc gacaccgtga
    32161 aacgccacac ccacacgttc actcaccacc gtgctacatt cccgtaaatg aataacaaaa
    32221 ggcaccggtg cgctatcttc ccctaccgca tgaaaccggt tactgctgat tttccccata
    32281 ttgaccgtca tttgtttatc accagcttca actcggcaag tttccgcaat aatgacgccc
    32341 tgaaattgca tatttccgcc gggcagcgtg gtattccatt tatttccggc cagagcaaac
    32401 attggcagca acgacgctaa tagaaataat ctcttccttt tcatcgttat ttttatcgca
    32461 caagggtggg catccctgcc cgtaatgacg tccctgaacc tgggtaggtt attgatactg
    32521 aaccttgaag gtcgcatccg cattagcagc acccggggtt gcctcgccga ttgcataata
    32581 acgcgcctgg aacggaatgg tgttggtacc gttattcaag gttgtttgtg cactaaatgt
    32641 cgcaccgtcc agcgccagcg cagcacccgt tctgtccagg atctgcacac caacttttgt
    32701 tgcgctaccc gcagctgaac tctgcaaagc taatacgtta ggatgagttc catcaattgc
    32761 ggtaccaagg aaggcaatag cagctttgtc tgcaacagta gtgtcacaat cgttcagttg
    32821 aatgttaaaa ccgacagcag aactggttgc tccttcttgt gccagcgatg ctgtacgaac
    32881 ctgccctaac tgaacagttt gatcaacaga gcctgcatca actgcgcaag cggcgttaac
    32941 aacctcccct ttaaagtgaa cggtcccacc atttaccgtc gtgggtgtag tttcagccag
    33001 agccgctgta gaactcaggg acagagccga cagaacaaca attgccagag ttttaatttt
    33061 catactgctt tcctttcaaa aaactatttc taaatcgaca tgggcagtcg ttctgtacac
    33121 tttgttttgt caacgagttt gactgccaac actgcacagt tttcccccaa aagatgaaac
    33181 atttggggcc attttgactc atagaggaaa gcatcgcgca caaacttttt cagtttattt
    33241 gttggcttaa tattctattg ttatctttat ttatagattt ttatattgca tgaggtggtt
    33301 tttgtagaga agaatgagga agatgcgtcg agccacagaa acgttagctt tacatttggc
    33361 ggaggtgatg tgaaattaat ttacaagaga aataatttac atatcaaaca gttagatgct
    33421 ttttatcgtt ttttaatatt tttatgcttg agaaaaaata cgtaacttat ttatgatatg
    33481 gacagtttgg ccccaattgt cttgtattta tttgtttttt aactttatta tcaatgagtt
    33541 aaatcaaacc tcttctcttt ttaatttttc gtttatgaga ttatttcttt cccataatcc
    33601 ggcaaaacga gcagcattac tggcggtata acgtacagta tggcgaatat ttcgatgccc
    33661 gagataatcc tgaattaaac gggtatcggc accacgctcc gccagttcat aaccgcaagc
    33721 atgccttaac atatgaggat gagtctgcgt tacggttcca gcttcaatac ctgcatcgcg
    33781 aataatgcga taggcctgct ggcgagaaag ccgactcccg cggcgagaaa taaatatagc
    33841 gtcggtccgg tcagcgcctt tccagttagc acgttcctgg gtccagcgtt ccacggcttc
    33901 acgctcatca aaacgtaacg ggtgaacggt agaaaatccg ttcttcagtc ggcgaatatt
    33961 tattcgacct tcattaaggt caagatcctg ataatgcaga tcaagcagtt cactaatacg
    34021 catcccatgc cgatatgcca acagaataag acaataatct ctggctcccg ttgccccgta
    34081 acaaaccgcc tgcatcatgg cctgaacttc tttaccggta agataacgac gtttactcac
    34141 aatagtagta ctcctaactg agatatattt gaatgaatac ctataggaaa cctcaatcgg
    34201 tcaaaattag cccaatagat aaaaagataa ccacacacga agt
//
LOCUS       DAZGQL010000056        33623 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.056, whole genome shotgun sequence.
ACCESSION   DAZGQL010000056 DAZGQL010000000
VERSION     DAZGQL010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33623
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.056"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(1..1144)
                     /gene="yjgL"
                     /locus_tag="ACHKIR_002899"
                     /pseudo
     CDS             complement(1..1144)
                     /gene="yjgL"
                     /locus_tag="ACHKIR_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313257.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjgL"
     gene            complement(1267..1719)
                     /gene="tabA"
                     /locus_tag="ACHKIR_002900"
     CDS             complement(1267..1719)
                     /gene="tabA"
                     /locus_tag="ACHKIR_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HFO9990606.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKANVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHTRYLDIQIVLKGQEGMTFSTQPAGTPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPARVRKAVVKMLMA"
     gene            complement(1864..2457)
                     /gene="bdcR"
                     /locus_tag="ACHKIR_002901"
     CDS             complement(1864..2457)
                     /gene="bdcR"
                     /locus_tag="ACHKIR_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFO9990607.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVSEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAIQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTILEE"
     gene            2528..3241
                     /gene="bdcA"
                     /locus_tag="ACHKIR_002902"
     CDS             2528..3241
                     /gene="bdcA"
                     /locus_tag="ACHKIR_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO9990608.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            3373..3768
                     /gene="yjgH"
                     /locus_tag="ACHKIR_002903"
     CDS             3373..3768
                     /gene="yjgH"
                     /locus_tag="ACHKIR_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFO9990609.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            4049..4183
                     /gene="pyrL"
                     /locus_tag="ACHKIR_002904"
     CDS             4049..4183
                     /gene="pyrL"
                     /locus_tag="ACHKIR_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HFO9990610.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            4187..5122
                     /gene="pyrB"
                     /locus_tag="ACHKIR_002905"
     CDS             4187..5122
                     /gene="pyrB"
                     /locus_tag="ACHKIR_002905"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HFO9990611.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            5135..5596
                     /gene="pyrI"
                     /locus_tag="ACHKIR_002906"
     CDS             5135..5596
                     /gene="pyrI"
                     /locus_tag="ACHKIR_002906"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HFO9990612.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            5669..6055
                     /gene="ridA"
                     /locus_tag="ACHKIR_002907"
     CDS             5669..6055
                     /gene="ridA"
                     /locus_tag="ACHKIR_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HFO9990613.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(6261..8957)
                     /gene="mgtA"
                     /locus_tag="ACHKIR_002908"
     CDS             complement(6261..8957)
                     /gene="mgtA"
                     /locus_tag="ACHKIR_002908"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFO9990614.1"
                     /translation="MFKEIFTRFIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGVYSRRYGWQ"
     gene            complement(9098..9151)
                     /gene="mgtL"
                     /locus_tag="ACHKIR_002909"
     CDS             complement(9098..9151)
                     /gene="mgtL"
                     /locus_tag="ACHKIR_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HFO9990615.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            9336..10283
                     /gene="treR"
                     /locus_tag="ACHKIR_002910"
     CDS             9336..10283
                     /gene="treR"
                     /locus_tag="ACHKIR_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor TreR"
                     /protein_id="HFO9990616.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTDITEEMLAYWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            10402..11823
                     /gene="treB"
                     /locus_tag="ACHKIR_002911"
     CDS             10402..11823
                     /gene="treB"
                     /locus_tag="ACHKIR_002911"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HFO9990617.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            11873..13528
                     /gene="treC"
                     /locus_tag="ACHKIR_002912"
     CDS             11873..13528
                     /gene="treC"
                     /locus_tag="ACHKIR_002912"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFO9990618.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKASPYRQFYIWRDGESETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPDDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRRLMTVGEMSSTSLEHCQRYATLTGSELSMTFN
                     FHHLKVDYPSGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQEINVEAALA
                     DESSVFYTYQKLIALRKQEAVLTWGDYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     TQPWQPGKMLGNWQLVMHNYEEASPQPCAMTLRPFEAVWWLQK"
     gene            13913..16051
                     /gene="nrdD"
                     /locus_tag="ACHKIR_002913"
     CDS             13913..16051
                     /gene="nrdD"
                     /locus_tag="ACHKIR_002913"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFO9990619.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRSQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            16209..16673
                     /gene="nrdG"
                     /locus_tag="ACHKIR_002914"
     CDS             16209..16673
                     /gene="nrdG"
                     /locus_tag="ACHKIR_002914"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HFO9990620.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDQIINDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVQRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(16718..17104)
                     /gene="cybC"
                     /locus_tag="ACHKIR_002915"
     CDS             complement(16718..17104)
                     /gene="cybC"
                     /locus_tag="ACHKIR_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HFO9990621.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVVEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEGKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(17287..18639)
                     /gene="pmbA"
                     /locus_tag="ACHKIR_002916"
     CDS             complement(17287..18639)
                     /gene="pmbA"
                     /locus_tag="ACHKIR_002916"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HFO9990622.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            18733..19284
                     /gene="yjgA"
                     /locus_tag="ACHKIR_002917"
     CDS             18733..19284
                     /gene="yjgA"
                     /locus_tag="ACHKIR_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HFO9990623.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIEQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            complement(19442..20815)
                     /gene="mpl"
                     /locus_tag="ACHKIR_002918"
     CDS             complement(19442..20815)
                     /gene="mpl"
                     /locus_tag="ACHKIR_002918"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HFO9990624.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLDPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            20991..21989
                     /gene="fbp"
                     /locus_tag="ACHKIR_002919"
     CDS             20991..21989
                     /gene="fbp"
                     /locus_tag="ACHKIR_002919"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HFO9990625.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            complement(22022..23017)
                     /gene="yjfF"
                     /locus_tag="ACHKIR_002920"
     CDS             complement(22022..23017)
                     /gene="yjfF"
                     /locus_tag="ACHKIR_002920"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
                     /protein_id="HFO9990626.1"
                     /translation="MIKRNLPLMITIGVFVLGYIYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPVYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQR"
     gene            complement(23004..24026)
                     /gene="ytfT"
                     /locus_tag="ACHKIR_002921"
     CDS             complement(23004..24026)
                     /gene="ytfT"
                     /locus_tag="ACHKIR_002921"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfT"
                     /protein_id="HFO9990627.1"
                     /translation="MPQSLPDTTPPKRRFRWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITSGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RNRDKT"
     gene            complement(24040..25542)
                     /gene="ytfR"
                     /locus_tag="ACHKIR_002922"
     CDS             complement(24040..25542)
                     /gene="ytfR"
                     /locus_tag="ACHKIR_002922"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfR"
                     /protein_id="HFO9990628.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKQQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAELSVPAIMNAIAA"
     gene            complement(25682..26638)
                     /gene="ytfQ"
                     /locus_tag="ACHKIR_002923"
     CDS             complement(25682..26638)
                     /gene="ytfQ"
                     /locus_tag="ACHKIR_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HFO9990629.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMIDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            26948..27478
                     /gene="ppa"
                     /locus_tag="ACHKIR_002924"
     CDS             26948..27478
                     /gene="ppa"
                     /locus_tag="ACHKIR_002924"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HFO9990630.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLIAVPHTKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(27851..28357)
                     /gene="mug"
                     /locus_tag="ACHKIR_002925"
     CDS             complement(27851..28357)
                     /gene="mug"
                     /locus_tag="ACHKIR_002925"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HFO9990631.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            28482..28557
                     /locus_tag="ACHKIR_002926"
     tRNA            28482..28557
                     /locus_tag="ACHKIR_002926"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28515..28517,aa:Ile,seq:cat)
     gene            complement(28657..29502)
                     /locus_tag="ACHKIR_002927"
     CDS             complement(28657..29502)
                     /locus_tag="ACHKIR_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFO9990632.1"
                     /translation="MSDITISRPELVTGHTDVICSTSIRHILAVRKSTLLQIDTLIRQ
                     LAEISAMTESIGGKTALDWAMKQDFRCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDAQARDTWYRSLEYDNFPEISEANILSTFEQLHQNKNEVFERGVINVFRGLS
                     WDYKTNSPCKFGSKIIVNNLVRWDRWGFHLNSGQQADRLADLERMLHLFSGKPIPDNR
                     ENITIHLDDHIQSVQGKEDYEDEMFSIRYFKKGSAHITFRKPELVDRLNDIIAKHYPG
                     QLSAI"
     gene            complement(29587..29784)
                     /locus_tag="ACHKIR_002928"
     CDS             complement(29587..29784)
                     /locus_tag="ACHKIR_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFO9990633.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAREDIRTLRQLQFLQQNR"
     gene            complement(29796..30284)
                     /locus_tag="ACHKIR_002929"
     CDS             complement(29796..30284)
                     /locus_tag="ACHKIR_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001499210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFO9990634.1"
                     /translation="MKLSLMLEADRINVQALNMGQIVVDVDGVNLTELINKVAENGYS
                     LRVVEESDQQSTCTLPPFATLAGIRCSTAHITEKDNAWLYSLSHQTSDVGESEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAGCLPGFPTF
                     DW"
     gene            complement(30281..30655)
                     /locus_tag="ACHKIR_002930"
     CDS             complement(30281..30655)
                     /locus_tag="ACHKIR_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFO9990635.1"
                     /translation="MKTLPVLPGQSASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSAGTSSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKHPEKR"
     gene            complement(30745..31098)
                     /locus_tag="ACHKIR_002931"
     CDS             complement(30745..31098)
                     /locus_tag="ACHKIR_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFO9990636.1"
                     /translation="MSDTLPDGNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAG
                     IRGLFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYARGLTCEADTLGSC
                     GYVYMAVYPTPETKK"
     gene            complement(31174..31395)
                     /locus_tag="ACHKIR_002932"
     CDS             complement(31174..31395)
                     /locus_tag="ACHKIR_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFO9990637.1"
                     /translation="MKIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAGAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(31464..31940)
                     /locus_tag="ACHKIR_002933"
     CDS             complement(31464..31940)
                     /locus_tag="ACHKIR_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFO9990638.1"
                     /translation="MQQLSFLPGEMTPGERSLILRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGSQVFSFAEHGLL"
     gene            complement(31956..32441)
                     /locus_tag="ACHKIR_002934"
     CDS             complement(31956..32441)
                     /locus_tag="ACHKIR_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO9990639.1"
                     /translation="MTTFSHSSTTPSVSVTTASGSDQPQLVATIVPDEQRISFWPQHF
                     GLIPQWVTLEPRIFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDGENWVLFNA
                     MNGNRAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            complement(32532..33350)
                     /locus_tag="ACHKIR_002935"
     CDS             complement(32532..33350)
                     /locus_tag="ACHKIR_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO9990640.1"
                     /translation="MRLASRFGYANQIRRDRPLTREELMQVVPSVFGEEKHTSRSENY
                     TWIPTITVLESLQREGFQPFFACQTRVRDPGRREYTKHMLRLRRAGQITGQHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVDRVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETLLESMR"
BASE COUNT         8248 a         8845 c         8434 g         8096 t
ORIGIN      
        1 aattaatatg atttattacg gagcatgcat gctctatctt tgcagaatca tcaaaatatt
       61 gcatgctttt attaaaacac cactccagta tttttacatc attattatat ggcaattcta
      121 aaaaagtcac caacatcgaa tggatgaccg gtgtaaggtt aacctttgca tcatctaagg
      181 aatcaataag aatatgagac aggttaatca tgatgtcatt aatttcattg ttaccttctt
      241 tgtcattatc aattccgtga taatttaatt cgtagagctt atctatttct tcaagcatac
      301 cattttcgcc cagtaagtat gaaatcgatt catcacatac atgaggcatt aatagcagat
      361 gttcgttcct tattgctaat aaggctttat tcaatgctta ttctaataaa tccgtaatcg
      421 tagaatcaac tgaaccttta caactcacct gcgagttatt taagtcctgc gagttattta
      481 actcctgaga atcatttaaa tcctgcgaat tatttaactc ctgcgaatta tttaactcct
      541 gtatattatt taacttctgt aaattattta acttctgatg attatttaat agacgcgcta
      601 tttccttgtt agcttcgcgt tggtaacacg ccagaacctt gttagtaatc aggtgtaatg
      661 aactatcatc ttttctttca aaagacttcc cttcataacg attagtcgaa ccatcagatt
      721 gttgaaatat aatagccgta tgtccttgac gagtccccat agtagtgata gtggaaagtg
      781 taatttctat ttccacctta gataaatccg ctcgttcccc ttcaggatta ttcatgtaaa
      841 acttcatttt ttcattgcca tcagtatctt tagaataaga tgaggactca atatgagcca
      901 tcatatttct acccgcttcg ttggagcgtt tgctaaaatc cacataaccc agggtaaata
      961 tctttgccaa aaactctaaa aatgctccag gagaaagaat attccttctg ctatcctgcc
     1021 ccccggcaat acgggcaaaa tcattcattc caacacgcca ggtatttaaa acttcacttt
     1081 tttgtttaaa attgtcggct aatgtaatgt gttgagaata attcaaatct gatattttgc
     1141 tcatgacgaa ctccttgagt taataagaac gtctaagcat tacaaatttt actcacaaaa
     1201 aaatatcaaa aaacgctctt ttcacaaact cgccgtaaac ctggagttat ttacggcgaa
     1261 aagtcatcac gccatcagca tcttcactac tgctttgcga acccgggctg gcgcgcccac
     1321 tgcgcacagc ggtttatgca cttcccccgg ataaaacaca acaaaatcac cttcattaag
     1381 gataactgtt ttctcatcaa cgccttccgg caaaaatgcg atgtctttat cagctaacca
     1441 gtcggtatcc ggcgtacctg caggttgtgt gctgaaggtc atgccttcct gaccttttaa
     1501 cacaatctga atgtcgagat agcgggtatg gtactccgca cggcgagctt cgtacggctc
     1561 ggtcatatct tccgagataa gataaaacag acgattgcct tcgatatcgt gcttgccctt
     1621 gggcgtttct gccgtaacgt ttgctttgat atgctcaatc gcctggcgta actcttgggg
     1681 tagccacggc tgaagattat gaatatttcc gatgatcatc ttttccactc cttaattaaa
     1741 acaacgtttc atttctatcg ttatacgaaa agatgatcaa ttccgctagc cttaacgcgt
     1801 gaattattgt cttatcacaa cttatcggca ggcgcgttcc cgcctttcgg gtcaaggccc
     1861 gcatcactcc tcgagaatag tttttatcgc ttcccccgcc agggcagctg ttgcacagag
     1921 ttgttcgatg gagtgcccct cccgtgcctt cgcagaaagc cctgacatca cggtactcat
     1981 aaaatcagtc acacattgtg cgctttgtgg atgccgcctg gcgatatagt cataaatggt
     2041 cgtttctgcg gcgtgataat attgaatggc aatatcacgc gcttgtggat catgactatg
     2101 aataccttca agaaccatac agccagcgca gccgccgttt tggctatatc tgcgcgccgc
     2161 ttcttttaat acctcaacca ggcactcgcc taccggacga tcatcacgaa gaatatcggc
     2221 aagcggaata gcttccgtac cgacatattc attgagtaca cgactaaaca acccagcctt
     2281 actgccaaaa gccgcgtaga ggctcggggg gttaatacca agataatcag taacttcaga
     2341 gacacttact gcatcgaaac ctttttggtg aaaaagcact tttgccgcag agactgcctg
     2401 ctcaggagcg aaacgtctgg ggcgacctgg aacgcgagat tgttttttag tgaccattac
     2461 aaaacttgtt gacagaaagt taaaacagtt ttgtaatgca tgttacataa taaatcaagg
     2521 agtccttatg ggcgctttta ccggtaagac agttctcatc ctcggtggta gccgtggaat
     2581 cggtgccgct atcgtacgtc gtttcgtcac cgatggagcc aatgtacgat tcacctatgc
     2641 ggggtcgaaa gatgccgctg aacgtctggc acaagagact ggagcgacag cagtattcac
     2701 agatagtgct gacagagacg ctgttattga tgtcgttcgt aagagcggcg cattggatat
     2761 cctggtggta aatgcaggta ttggcgtctt tggcgatgcc ctggaattaa atgccgacga
     2821 tattgatcgc cttttcaaaa tcaatattca tgctccttat catgcctctg ttgaagccgc
     2881 ccggcagatg cccgaaggcg ggcgcatctt aatcatcggc tccgtgaatg gcgatcgtat
     2941 gcctgttgca ggcatggctg cttatgccgc cagtaaatct gccctgcaag gcatggcgcg
     3001 cgggctggcc cgtgattttg gaccgcgagg gatcaccatc aacgtcgtcc agccaggacc
     3061 aattgatacc gacgctaatc ccgccaacgg gccaatgcgc gatatgttgc atagttttat
     3121 ggccatcaaa agacatgggc aaccggaaga ggtcgctggt atggtcgcat ggttagcagg
     3181 gccagaagcc agttttgtta ccggcgcgat gcataccatt gatggcgcgt ttggcgcata
     3241 accgactacg ctcaattaag cccagccatt atccatgatg tctgggtttt gtttactaac
     3301 gtcgtccccc taaaagcggc tcccggtaaa tataaatctt ctgttcatgt cgtaaatctt
     3361 gaggataacc aaatggtaga aagaaccgct gttttccctg ctggccgaca ttcactgtat
     3421 gctgagcatc gttattctgc ggctattcgt tccggcgatt tgctgtttgt ttccgggcaa
     3481 gtaggaagtc gagaggacgg aacaccagaa cccgattttc agcaacaagt cagactggca
     3541 tttgataatt tgcatgcgac cctggctgct gcgggatgca cttttgacga tatcattgat
     3601 gttacgagct ttcataccga tccagaaaaa caatttgaag acatcatgac ggtcaaaaat
     3661 gaaatattta gcgccccacc ttatccaaac tggacggcgg tgggtgttac ctggctggca
     3721 ggctttgatt ttgaaattaa agtgatagcg cgcatccctg agctataagc aatagtgtta
     3781 gccgttcgtt ttcacactcc gccctataag tcggatgaat ggaataaaat gcatatctga
     3841 ttgcgtgaaa gtgaaaaaga aaaaagcagg aaatgtctgc aattattgat accgaacgac
     3901 agttctcccc cagaatcaca tcaaataaaa atgcatgtat tttgactttt aattcaaata
     3961 aaccgtttgc gctgacaaaa tattgcatca aatgcttgca ccgcttctga cgatgagtat
     4021 aatgccggac aatttgccgg gaggatgtat ggttcagtgt gttcgacatt ttgtcttacc
     4081 gcgtctgaaa aaagacgctg gcctgccgtt tttcttcccg ttgatcaccc attcccagcc
     4141 cctcaatcga ggggcttttt tttgcccagg cgtcaggaga taaaagatgg ctaatccgct
     4201 atatcagaaa catatcattt ccataaacga ccttagtcgc gatgacctta atctggtgct
     4261 ggcgacagcg gcgaaactga aagcaaaccc gcaaccagag ctgttgaagc acaaagtcat
     4321 tgccagctgt ttcttcgaag cctctacccg tacccgcctc tctttcgaaa cttccatgca
     4381 ccgcctgggt gccagcgtgg tgggcttctc cgacagcgcc aatacatcac tgggtaaaaa
     4441 aggcgaaacg ctggccgata ccatttcggt tatcagcact tacgtcgatg cgatagtgat
     4501 gcgtcatccg caggaaggtg cggcgcgcct ggccaccgaa ttttctggca atgtaccggt
     4561 actgaatgcc ggtgatggct ccaaccaaca tccgacgcaa accttgctgg acttattcac
     4621 cattcaggaa acccaggggc gtctggacaa tctccacgtc gcaatggttg gtgacctgaa
     4681 atatggccgc accgttcact ccctgaccca ggcgctagcg aagttcgacg gcaaccgttt
     4741 ttactttatc gcgccggatg cgctggcaat gccgcaatac atccttgata tgctcgatga
     4801 aaaagggatc gcctggagtc tgcacagctc tattgaagaa gtgatggcgg aagtagacat
     4861 cctgtacatg acccgcgtgc aaaaagagcg tctggacccg tccgagtacg ccaacgtgaa
     4921 agcgcagttt gttcttcgcg ccagcgatct ccacaacgcc aaagccaata tgaaagtgct
     4981 gcatccgctg ccgcgtgttg atgagattgc gacggatgtg gataaaacgc ctcacgcctg
     5041 gtacttccag caagcaggca acgggatttt cgctcgccag gcgttactgg cactggttct
     5101 gaatcgcgat ctggtactgt aaggggaaat agagatgaca cacgataata aattgcaggt
     5161 tgaagctatt aaacgcggca cggtaattga ccatatcccc gcccagatcg gttttaagct
     5221 gttgagtctg ttcaagctga ccgaaacgga tcagcggatc accattggtc tgaacctgcc
     5281 ttctggcgag atgggccgca aagatctgat caaaatcgaa aatacctttt tgagtgaaga
     5341 tcaggtagat caactggcat tgtatgcacc gcaagccacg gttaaccgta tcgacaacta
     5401 tgaagtggtt ggcaaatcgc gccccagtct gccggagcgc atcgacaatg tgctggtctg
     5461 cccgaacagc aactgtatca gccatgccga accagtttct tccagctttg ccgtgcgaaa
     5521 acgcgccaat gacatcgcgc tcaaatgcaa atactgtgaa aaagagtttt cccataatgt
     5581 ggtgctggcc aattaattgc ggttggtaat aaaagtctgg ctccctataa tgagccagac
     5641 tttttaccgc tgtaataaag gagaaatcat gagcaaaact atcgcgacgg aaaatgcacc
     5701 ggcagctatc ggtccttacg tacagggcgt tgatctgggc aatatgatca tcacctccgg
     5761 tcagatcccg gtaaatccga aaacgggcga agtaccggca gacgtcgctg cacaggcacg
     5821 tcagtcgctg gataacgtaa aagcgatcgt cgaagccgct ggtctgaaag tgggcgacat
     5881 cgttaaaacc accgtgttcg tgaaagatct gaacgacttc gcaaccgtaa acgccactta
     5941 cgaagcgttc ttcaccgagc acaatgccac cttcccggca cgttcttgcg ttgaagttgc
     6001 ccgtctgccg aaagacgtga agattgagat cgaagcaatc gctgttcgtc gctaatcttg
     6061 atggaaatcc gggctatcat gcccggatta agtctgatga caaaagcaaa atcgcccgat
     6121 gcgctacgct tatcaggcct acgtgattcc tgcaatttat tgaatttgta ggccggattg
     6181 ggcgtttacg ccgcatccgg catgaacaaa gctcactttg tctgcaatct gaatccgggc
     6241 caccatgccc agtttattct ttactgccag ccgtaacgac ggctatagac ccctttcacc
     6301 aactgggtta acgtcatata ccctgccaga atcgcaacca gccacgggaa atagcttaac
     6361 ggcagcgcct gcagctgcag ataactggcc agcggtgaaa acggcaatgc gatcccgaca
     6421 atcatcacga tcacggtcat gatcattaac ggccacgatg cacagctctg aataaacggt
     6481 acacgacggg tacggatcat atgcacaatc agcgtttgcg acagtaagcc caccacaaac
     6541 cagcccgact ggaacagcgt ttgcgtttcc ggcgtgttgg catggaatac ccaccacatc
     6601 aggcaaaacg tcagaatatc gaagatcgag ctgatcggtc cgaagaagat cataaagcgc
     6661 cccagatccg ccggattcca acgctgcggc ttttgaattt gctcgtcgtc gacgttatca
     6721 aacgggatcg ccacctgtga cacatcgtac agcaagttct gaataagcaa gtgtaacggc
     6781 aacatcggca ggaagggcaa gaaagcactc gccaccagca cgctgaacac attaccgaag
     6841 ttagaactcg ccgtcatttt gatgtatttc agcatgttgg cgaaagtgcg acgtccctca
     6901 ataaccccct cttccagcac catcaggctt ttttccagca ggatgatatc agccgcttca
     6961 cgggcaatat ctaccgcgcc gtccacagaa atgccgatat ccgcagcgcg taaggcgggc
     7021 gcatcattaa taccatcgcc cataaagcca accacatgcc cttcgcgctt cagtaaggtc
     7081 acaatgcgtt ctttatgcat cggcgtcagg cgggcaaaca gcgtggtgcg ctgtgcgaga
     7141 tttgccagtt catcgtcaga tagcgtttca atatcactac caatgaccac ctctcccgca
     7201 tccagcccca cttcatggca cactttcgct gcaactaact cactgtcgcc agtaaggatt
     7261 tttacggtaa ttccactcgc ttttaatgcc ttcagtgccg gagcggttgt ctcttttggc
     7321 ggatcaagga aagcaatata tccttcgagg atcaggtcgg attcatccgc ccgctggtaa
     7381 tctccttcac gcgctggcag gtatttcgtc gccaccgcaa ccacgcgcag cccctgacga
     7441 ttcagcgtat cagtaacccg cttaatctta cgcagcatga tgtcatcgag cggcacaatc
     7501 tcgccattgt gacgcacctg cgaacacaca ttgaggattt cctgcaatgc acctttgcaa
     7561 accagctgat ggtgttcggt attttctgcc actaccaccg acatccgacg acgctcgaaa
     7621 tcaaacggaa tctcatcaat tttttgccaa cggctggcca gcgaacgcgc tgactcttca
     7681 tccgtacctt cgagcaccgc ggtatcaagc aggtttttaa gtccggtctg gtaatgactg
     7741 ttcaaccacg cgctatgcag cacgcgttcg ctggttttac cggagatatc ggtatgattc
     7801 tccagcacaa ttttgtcctg cgtcagggtg ccggttttat cagtgcacag aatatccatt
     7861 gcgccaaagt tctgaatggc atccagatgt ttgacgatca ctttctgttt cgacagcttt
     7921 actgcgccgc gcgccagcgt cgaggtaaca atcatcggca acatttctgg cgttaagcct
     7981 accgcgaccg aaagcgcaaa cagtgccgct tcccaccagt cgcctttggt gtaaccgttg
     8041 ataagcaaca ccaccggagc catcaccagc ataaagcgaa tcagcagcat actgacgcgg
     8101 ctaatccctt gctgaaaggc attcggctcg ctttcctgct cactaacacg ccccgccagt
     8161 tgaccaaacc aggtattggc ccctgtggca atcaccattg cttgtgccgt accgctcacc
     8221 acggtggtgc ccataaaaca cagggtgtcg cactccagcg gattgctgtg ctccggctgg
     8281 cgagtagtag cggctttttc cacgggcaga gactcaccgg ttaacgacgc ctgagcgacg
     8341 aacagatccc gcgcctgcaa gatacgtaaa tctgccggga tcatatcccc tgccgccagt
     8401 tttataatat cgccgggcac cagttggtcg atcgggatct ccagccagcc attttcgcct
     8461 ttgtcgttaa ttacgcgcag caccgtcgca gtattgctga ccatcgcttt cagggcatct
     8521 gccgctttag tggaacgtgc ttcctgaata aagttcagca acgtagaaat agcgaccatt
     8581 agcgcgataa cgcccgcggc aaataaatct tccgtggcgt aagaaatagc gccgagaatg
     8641 gtgagtaaaa tattaaaggg gttgcgatag cagacccata aatgaaccca ccacggcgac
     8701 ggttgttgtg cgggtaattt attttcacca tgttgttcgc gggcagattc cacttccgcc
     8761 tgatttaatc cttccggatg ggtgtcgaac gttttccaca attcttcttc gggcatcacc
     8821 gccattttca ggcaatgcgc acttaaggac ggcgggaccg ccgtattcac tgtctgctgc
     8881 gcgccaggca atggatcgcg atgaaccaga cgggaaggta aatggcgaat gaaccgggta
     8941 aaaatttctt taaacataag gagtccctcc gcactgtctg aaacagtaca gaaatgtctt
     9001 caggcgtggc gaagccttac ctgaaggtaa taaagctgag cagggacgtt aaaacgtcac
     9061 ggccttacgc atccggtaac gccgtgaaaa gacgggctta ccggaaaagt tttaatctcc
     9121 gtcgagggag aggcgtggga tcaggttcca tataacctcc ggtaagtgaa aaaagcgccg
     9181 caaataataa ggagaatcca ataatattcg tagcatttat ggcgatataa tacctgctgg
     9241 caactaaacc aacgataaac cagactttac ccattgctga atgcacaggt aaagttaggc
     9301 tcaaataatt aaacaacacg ttatgggaca acaggatgca aaatcggctg accatcaaag
     9361 acatcgcgcg cttaagcggc gtggggaaat ctacggtttc ccgggtgctg aataacgaaa
     9421 gcggcgtgag ccagcgcacg cgcgagcgtg ttgaagcagt gatgaatcag catggatttt
     9481 ccccttcccg ctctgcgcgc gctatgcgtg ggcaaagcga taaagtggtc gccatcattg
     9541 ttacccgtct ggattcgttg tcagaaaatc tcgccgttca aaccatgctg ccagcgttct
     9601 atgaacaagg ttacgatcca atcatgatgg aaagtcagtt ttccccacaa ttagttgccg
     9661 aacatttggg ggtgctgaaa cggcgtaata tcgacggcgt agtgctgttc ggttttaccg
     9721 acataacaga agaaatgtta gcctactggc agtcatcgct ggttctgctg gcgcgtgacg
     9781 caaaaggctt tgcttcggtc tgttatgacg acgaaggggc aatcaagatc ctgatgcaac
     9841 ggctgtatga ccagggacat cgtaatatca gttatctcgg cgtgccgcac agtgacgtga
     9901 cgaccggtaa gcgacgtcac gaagcctacc tggcgttctg caaagcgcat aaattgcatc
     9961 ccgttgccgc tctgccaggg cttgctatga agcaaggcta tgagaacgta gcaaaagtga
    10021 ttacgcctga aactaccgcg ttactgtgcg caaccgacac gctggcactt ggcgcaagta
    10081 aatacctgca agagcaacgc atcgacacct tgcaactggc gagcgtcggt aatacgccgt
    10141 taatgaaatt cctccatccg gagatcgtca ccgtagatcc cggttacgcc gaagctggac
    10201 gccaggcggc ctgccagttg atcgcgcagg taaccgggcg cagcgaaccg caacaaatca
    10261 tcattcccgc caccctgtcc tgatcgtttc ctgaacgata aattgtgatc ttcgctgcgt
    10321 ttcgggaacg ttcccgtttt taaatttttc agcgcaatat attctgcagc caaccaaaaa
    10381 tgtcatctgc catggggctt tatgatgagc aaaataaacc aaacggatat cgatcggttg
    10441 attgaactgg tcggcgggcg cggcaatatt gcgacggtga gccactgtat tactcgtctg
    10501 cgctttgtcc tcaaccaacc ggccaatgcc agaccgaaag aaattgagca actccccatg
    10561 gtgaaaggct gtttcaccaa tgccgggcaa tttcaggtgg tgattggcac caacgtgggt
    10621 gattactatc aagcactgat agcgtcaacc ggacaggcgc aggttgataa agagcaggta
    10681 aaaaaagccg cccggcagaa tatgaaatgg catgagcagt tgatctctca tttcgcggag
    10741 attttcttcc cgttgctgcc cgcgttgatt agcggcggtt tgatcctcgg ttttcgcaat
    10801 gtgatcggcg atttgcccat gagcaacggt caaacgctgg cgcaaatgta cccttccctg
    10861 caaacgatct acgattttct gtggttgatc ggtgaagcga tcttcttcta cctgccggtc
    10921 gggatttgct ggtcagcggt gaaaaaaatg ggcggcacgc cgatccttgg tatcgtgctt
    10981 ggcgtaacgc tggtttctcc acagctgatg aacgcttatc tgctcgggca gcagctgccg
    11041 gaagtgtggg actttggcat gttcagcatc gccaaagtgg gctatcaggc gcaggtgatc
    11101 ccggcactgt tagccgggct ggcgctgggc gttattgaaa ctcgtcttaa gcgcatcgtg
    11161 ccggattacc tctatctggt ggtggtgccc gtttgttcgc tgatcctcgc ggtgttcctc
    11221 gcccatgcgc tgattggtcc gtttggtcgc atgattggcg atggcgttgc ctttgcggta
    11281 cgtcacctga tgaccggcag ctttgctccg attggcgcgg cattgtttgg cttcctgtac
    11341 gccccgctgg tgatcaccgg cgtacatcag accacgcttg ctattgattt gcagatgatt
    11401 caaagcatgg gtggtacgcc agtgtggccg ctgattgcgc tgtcgaatat cgctcagggt
    11461 tccgccgtga taggcattat catttccagc cgcaagcaca atgaacgcga gatctccgtg
    11521 cctgccgcta tctccgcctg gcttggagtc actgagcctg ctatgtacgg catcaacctg
    11581 aaatatcgct tcccgatgct gtgcgcgatg attggttctg gtctggcagg attgctatgc
    11641 ggcctgaacg gcgttatggc gaatggcatc ggcgtaggcg gcctgccggg aattctctcg
    11701 attcaaccga gctactggca ggtgtttgcg ctggcaatgg ctatcgccat catcatcccg
    11761 attgtactca cctcgtttat ctatcagcgg aaataccgcc tgggcacgct ggacattgtt
    11821 taattttctt cggggcgcaa ttgcgccccc tcgcatttgc aggaataacg taatgactaa
    11881 tcttccccac tggtggcaaa acggcgttat ctaccagatt tatccaaaga gttttcagga
    11941 caccacgggt agcggcaccg gcgatttacg tggcgttatc cagcgcctgg actatctgca
    12001 taaactgggc gttgatgcca tctggctaac ccccttttat gtctctcccc aggttgataa
    12061 cggttacgac gtagcgaact atacggctat tgatcccacc tacggcacgc tggacgattt
    12121 tgacgaactg gtgacacagg caaaatcgcg cgggattcgt atcattctcg atatggtgtt
    12181 taaccatacc tctacccaac atgcctggtt tcgcgaggcg ctgaacaaag caagccctta
    12241 ccgccagttt tatatctggc gcgatggaga atcagaaacg ccaccgaaca actggcgttc
    12301 aaaatttggc ggtagtgcgt ggcgctggca tgcggaaagc gaacagtact atttgcatct
    12361 ctttgcacca gaacaggcgg atctcaactg ggagaatcca gcggtacgcg cagagctgaa
    12421 aaaagtctgt gagttctggg ccgatcgtgg ggtcgacggg ttgcgcctgg atgtggtgaa
    12481 tctgatctcc aaagacccgc gtttccctga tgacctggat ggcgacgggc gtcgcttcta
    12541 caccgacggg ccacgagcac acgagttttt gcacgagatg aaccgcgatg tgtttacgcc
    12601 acgcaggtta atgaccgtag gtgaaatgtc ctccaccagc cttgagcatt gccagcgata
    12661 tgcgacactg acaggcagtg aattgtcgat gacctttaat tttcatcacc tgaaagtcga
    12721 ttatcccagt ggtgaaaaat ggacgctggc taaacctgac tttgtggcgt tgaaaacatt
    12781 gttccgccac tggcaacaag gaatgcacaa cgtggcatgg aatgccttgt tctggtgtaa
    12841 ccacgatcag ccgcgcattg tttctcgctt tggtgatgaa ggtgaatacc gcgtgcctgc
    12901 ggcaaaaatg ctggcgatgg tgctgcatgg catgcaggga acgccgtata tctaccaggg
    12961 cgaagagatt ggcatgacca acccgcattt cacgcgcatt actgactatc gcgacgtgga
    13021 gagcctcaat atgtttgccg agctgcgcaa cgatggtcgt gatgccgacg agttattggc
    13081 aatcctcgcc agtaaatccc gtgacaacag tcgcacaccc atgcaatgga gcaacggcga
    13141 taatgccggg tttacggctg gcgaaccgtg gattggcctg ggcgataact atcaggaaat
    13201 caacgtagaa gcagcgctgg ccgatgagtc ctcggtgttt tacacctacc aaaagttaat
    13261 cgcactgcgt aagcaagaag ccgtcctgac atggggcgat taccaggatc tgctgccaaa
    13321 cagccctgta ttgtggtgct atcgccgtga gtggaagggg caaaccttac tggtcattgc
    13381 caaccttagc cgtgagaccc aaccctggca gccagggaaa atgctcggca actggcagct
    13441 tgtgatgcat aactacgaag aagcctcacc acaaccctgt gccatgactt tacggccttt
    13501 tgaggctgtc tggtggttac agaagtaagt atccgctaag ccccggtaat actggtcatt
    13561 ccggggtttt gcctttcagc atgttgattt tataatcaaa aaattcatta aaaaatttac
    13621 ataacgctgt agcgcccgtc atccgtacgc tctgcttttt actttgagct acatcaaaaa
    13681 aagttcaaac atccttgatg caaagcacta tatatagact ttaaaatgcg tcccgaccca
    13741 atatgttgta ttaatcgact ataattgcta ctacaactcc ccacgaaaaa ggtgcggcgt
    13801 tgtggataag cggatggcga ttgcggaaag cgccggaaaa cgaaacgaaa aaaccggaaa
    13861 acacctttcc caatttctgt ggataacctg ttcttaaaaa tatggagcga tcatgacacc
    13921 gcatgtgatg aaacgagacg gctgcaaagt gccgtttaaa tcagagcgca tcaaagaagc
    13981 gattctgcgt gcagctaaag cagcggaagt cgatgatgca gattattgcg ccactgttgc
    14041 cgcggttgtc agcgagcaga tgcagggccg cagccaggtg gatatcaacg agatccagac
    14101 cgccgttgaa aaccagctta tgtcgggtcc atacaaacaa ctggctcgtg cttacatcga
    14161 gtaccgtcac gatcgcgaca ttgaacgtga aaaacgcggt cgcctgaatc aggagatccg
    14221 tggtctggtt gagcagacca acgcctcgtt gctcaacgaa aacgccaaca aagacagcaa
    14281 ggtgattcca acccagcgcg acctgctggc cgggattgtg gctaaacact atgcacgcca
    14341 gcacctgctg ccgcgtgacg tggtgcaggc acatgaacgt ggtgatattc actatcacga
    14401 tctcgattac tcaccgttct tcccgatgtt caactgcatg ttgatcgacc tgaaaggcat
    14461 gctgacccag ggctttaaaa tggggaacgc cgagattgaa ccgccgaagt cgatctctac
    14521 ggcaaccgcg gtaactgcgc agattattgc ccaggtcgcc agccatattt atggcggcac
    14581 caccattaac cgtatcgatg aagtgctggc accgtttgtc accgccagct acaacaaaca
    14641 tcgcaagacc gcagaagagt ggaacatccc ggacgccgaa ggctacgcta actctcgtac
    14701 catcaaagag tgctacgatg ccttccagtc gctggagtac gaagtaaaca ccctgcacac
    14761 cgccaacggt caaacgccgt ttgtaacctt tggttttggt ctgggcacca gctgggagtc
    14821 gcgcctgatt caggaatcta tcctgcgtaa ccgtatcgcc ggcctcggta aaaaccgtaa
    14881 aacggcggtg ttcccgaaac tggtgtttgc gattcgcgat ggcctgaacc ataaaaaagg
    14941 cgatccgaac tacgacatca aacagctggc gctggagtgc gcaagcaagc gcatgtatcc
    15001 ggatatcctg aactacgatc aggtagtgaa agtcaccggt tcgtttaaaa ccccgatggg
    15061 ctgccgcagc ttcctcggcg tgtgggaaaa tgaaaacggc gagcagatcc acgatggtcg
    15121 taacaacctc ggcgtgatca gcctgaacct gccgcgtatt gctctggagg caaaaggcga
    15181 tgaagccacc ttctggaagc tgctggatga acgtctggtg ctggcacgta aggcgttgat
    15241 gacccgtatc gctcgtctcg aaggcgtgaa agcgcgcgtg gccccgatcc tctatatgga
    15301 aggtgcttgt ggcgtgcgtc tgaatgctga cgacgacgtt tctgaaatct tcaaaaacgg
    15361 tcgtgcgtct atttcgctgg gttacatcgg catccacgaa accattaacg cgctgttcgg
    15421 cggcgagcat gtttacgaca acgagcagct gcgcgcgaaa ggtattgcga ttgttgagcg
    15481 tctgcgtcag gcggtggatc agtggaaaga agaaacgggt tatggtttca gtctctacag
    15541 cacgccaagt gaaaacctgt gcgaccgctt ctgccgtctc gataccgctg agtttggcgt
    15601 ggtgccgggc gtgaccgata aaggttacta caccaacagt ttccaccttg atgtggagaa
    15661 aaaggtgaac ccgtatgaca agatcgactt tgaagcgcct tacccgccgc tggcgaacgg
    15721 tggtttcatt tgctacggcg agtatccaaa cattcagcac aacctgaagg cgctggaaga
    15781 tgtctgggat tacagctatc agcatgtacc gtattacggc accaatacgc cgatcgatga
    15841 gtgctacgag tgtggcttta ccggtgagtt cgagtgcacc agcaaaggct tcacttgtcc
    15901 gaaatgtggt aaccatgacg cctcccgtgt gtcggtgact cgccgcgtgt gcggatattt
    15961 aggtagcccg gatgcacgtc catttaacgc cggtaagcag gaagaagtta agcgccgcgt
    16021 taaacatttg gggaatgggc agataggtta atcttcactc tgcgttaagc aaatgccgga
    16081 tgcggcgtaa acgccttatc cggcctacat ttggcaacgg ctgtaggcct gataagacgc
    16141 gacagcgtcg catcaggcgt tgattactgg atgcagccta cacgcggact gataagccag
    16201 gcgaaataat gaattatcat cagtactatc ctgtcgacat cgtcaacggc cccggcactc
    16261 gctgcaccct gtttgtctcc gggtgtgttc atgaatgccc cggttgctat aacaaaagca
    16321 cctggcgggt aaattccggt cagccattta ccaaagcaat ggaagaccag atcattaacg
    16381 atctgaatga cactcgcatc aaacgccagg gtatttccct ctccggcggc gatccgctgc
    16441 atccgcaaaa cgtgccggat attctgaaac tggtacaacg catccgcgcc gaatgtccgg
    16501 gtaaagacat ctgggtgtgg acaggctata aactcgacga actcaacgct gcgcaaatgc
    16561 aggttgttga tttgattaac gtgctggtcg acggcaaatt tgtgcaggat ttaaaagacc
    16621 cttccctgat ctggcgcggc agcagcaatc aggtggtgca tcatttgcga tgataagttc
    16681 cctgttacct gagtgcaggc aacagggaaa taaggaatta acgatacttc tggtgatagg
    16741 cgttgcgggt cgttttcagt tgctctgcgg cagcctgcgc ttcttttact ttaccttcat
    16801 ttgccagctt cagcgcgtcg tcaatctgac cgaccagaat atcgaaaccg tggcggaagt
    16861 ctttcatttc cgggctgtcc ggtgatttgc cttcgagctt cggcggcgtc gctttttgcg
    16921 catccagcgc tgcggcgcgc atcttcgtta acgcgtcttt gacttgcgcc gcgttatcgg
    16981 ctttttcgac cacttttaaa ttgtcgttga gggtttccat attgtcttca agatcagcgg
    17041 caaacgacgc cgaactgaat accaacgagg agactgcaag aatagctaac aggcttttac
    17101 gcattgctca cttccttttt tatttacact tcatccttca cactgcctca tcgacggcta
    17161 cgctcattcg cccgagtcac gtactgcgag tacgctcccc ggtcctcatt cactttccgt
    17221 ctcgatgcag tgcgaatgat tttgtgtaat tttttagttt atagcgcggc aggtcgcgcc
    17281 agttttttac tgcccggcga ttttcatctc cggcaacagc acagaaccac actgtatatt
    17341 actgcgtgtt tcaatatcgt taccgacggt gacaatattg cgccacatat cttttaaatt
    17401 acctgcgatg gtgatttcgc taaccggata ctgaatttcg ccattctcta cccagaagcc
    17461 cgctgcacca cgggaataat caccggtaat ggcactcacg ccctggccca tcagctccgt
    17521 caccaccagc ccggtgccca tctctttgag catttgttcg aagcttagcc cttgtccggc
    17581 aatccgccag ttgtgaatac cgcccgcatg tccggtgctt ttcagcccca gtttccgcgc
    17641 cgaatagctg gtcagcagcc actgagtcag gatgccatct ttaataatat cgcgacgctc
    17701 ggtgcgcaca ccttcgctgt cgaatggcgt agaggccagc cctttcagca gatgcggatg
    17761 ctcttcaatg gtcagccagt ccggcagaat ttgtttaccc agcgaatcca gcaggaaggt
    17821 agatttacgg taaaccgatc cacccgctat cgccccaacc agatgcccaa aaagcccggt
    17881 tgccacttca ttggcaaaaa tgaccggcgc tttcatggtg gagagtttac gcggtgacag
    17941 acgcgataaa gtgcggcgag cacagtcggc cccgacccac tctggcgttt gcagatcgct
    18001 catcgcacga ccaatggtgt aggcgtaatc acgctccata tcgccatttt cttcggcaat
    18061 tacacagctg gagagcgaat gacgcgtaga gcagtaaccc tgcaacatgc cgtggctgtt
    18121 gccaaaaact ttgacaccgt agtggctgtt aaagctgcca ccttcggtat tggtgatgcg
    18181 tttgtccgcc tgcaatgctg cctgttctgc gcgggccgcc agttcaatgg cttcatccgg
    18241 ggaaacttcc gcagggtgga acaagtcgag atccggtgcg tcaaaggcca gcagctcttt
    18301 gtctgccacg ccggcacagg gatctggcga ggtataacgg gcaatatcta gcgccgcctg
    18361 tacggtgcgg gcaatggcct gcgggcttaa atcggtagac gatgcgctac ctttgcggtt
    18421 ctggtgataa acagtgatcc ccaacgcgcc atcgctattg aattcaacat tctccacttc
    18481 accataacgc gtgcttacgc taatgccggt ggtcttgctg acggcaactt ctgcaccgtc
    18541 cgatttgcct gaggccaact ccagcgcagt cgaaactgct tcttccagaa tcttgcgctg
    18601 cgcttcaact tgagagatta ctttcattgc aagtgccata atgtagagag tttctctgaa
    18661 gtctaacaga gaaccgtttt tcagtgcgca tcttaactgg taacattagc ctcttttttt
    18721 aaggagcctg agatgactaa gcagcccgaa gactggctcg acgacgttcc cggtgatgac
    18781 atcgaagacg aagacgatga aattatctgg gtcagtaaaa gtgaaattaa acgcgatgcc
    18841 gaggagctaa aacgccttgg cgcggaaatt gttgatctgg ggaaaaacgc gctggataag
    18901 atcccgttag atgcggatct gcgcgcggct attgagctcg cccagcgtat taagatggaa
    18961 ggtcgccgcc gccagttgca gctcatcggt aagatgctgc gccagcgcga tgttgagcct
    19021 attcgtcagg cgctggataa gctgaaaaac cgccacaacc agcaagtggt gttgttccat
    19081 aaactggaaa acctgcgcga tcgtctgatc gagcaaggtg atgacgccat cgctgaagta
    19141 ttgaatctgt ggcccgatgc cgatcgtcag caactgcgta ctctgatccg taacgcgaag
    19201 aaagagaaag aagggaataa gccgccgaaa tccgcgcgcc agattttcca gtatctgcgt
    19261 gagttagcgg aaaacgaagg gtaattttcg ttgttgatgt gagtatggtt tgccggatgc
    19321 ggtgcacctg taggcctgat aagacgcatc aagcgtcgca tcaggcacta tgctcaaatg
    19381 ccggatgcgg cgtgaacgcc ttatccggcc tacacacact ctatcaggct gaggctgtga
    19441 attactgcgc ggcttccgct ttcttcgcta acccatccag cagtttctga tggatcccac
    19501 caaaaccgcc gttactcatc accagaatgt gatcgcccgg ctgagcggtt ttcaccacca
    19561 tatctgccag cgtatccaca tcgccgctcc agtgtgcagg ctgaacgcag gcttctgcca
    19621 cttctgccac ctgccacgga atatgcgccg gttgcagcag gaagacttca tcggcacgac
    19681 ctaatgaagg tgccagatcg tctttgcaga tccccatttt catggtgttt gagcgcggtt
    19741 ccagcacggc aataatgcgc gccgtaccac caactttgcc acgcagcgcc gcaagcgttg
    19801 ccagaatcgc cgtcgggtga tgggcaaaat cgtcatatac cgtcacacca ttcgcttcac
    19861 cacgcaactc cagacggcga cgagcattaa taaacgaacc cagcgcgtta gcggcatctg
    19921 ccggcgctac accaacatgg cgagccgccg caatcgccat caggccattg tgcatattat
    19981 gttcgcctac cagcgaccat ttcacttcgc ccactttttc gccatccagc aagacttccc
    20041 attcggaagc atcggtggtc agctttttcg cctgccagtg accttgctcg cccaccagct
    20101 cctgctcgct ccagcagccc atcgccatgg tctgtttcag gttgatgtca ttttccggcc
    20161 agataatacg gccctgcccc ggaacgatac gcaccagatg gtggaactgt ttctggatcg
    20221 ctttcaggtc gtcaaagata tcggcgtgat cgaactcaag gttgttgagg atcagcgtac
    20281 gcgggcagta atggacaaat ttagagcgtt tgtcgaagaa ggcgcagtca tactcatccg
    20341 cttcgataac aaagaagtcg ctttcgccca gacgcgccga aacctcaaag ttccccggca
    20401 caccgccgat cacaaatccc ggtttgtaac cgcactgttc cagaatccag gttgccattc
    20461 ccgcggtggt ggttttgcca tgtgtaccgg caacggccag cacccagcgg tcgcgcagca
    20521 caaaatcgtg cagccactgc ggacctgaca tataagggat gtttttttcc agtaccgctt
    20581 ccacacacgg atttccacgg gtcatggcgt taccaataat caccagatcc ggctgtggat
    20641 cgagctggct ggcatcgtaa ccctgaatca gttcaatgcc ttgcttctca agtaaggtgc
    20701 tcatcggcgg atacacattg gcgtccgaac ccgttacttc atggcctaac tggcgcgcca
    20761 gcatcgccag accgcccata aacgtgccac aaattcctaa aatatgaatg cgcatacgtc
    20821 actatccttt tttaatctgg cgctcatttt acgcatatgt cgggcaagtg agaaacgcat
    20881 ttcaggataa tccgtaattt gctggcgcga ttcacctgag cgcaacattg ggattatttg
    20941 ttaagattgt tgcggtcgct ttactccata aacattgcag ggaaagtttt atgaaaacgt
    21001 taggtgaatt tattgtcgaa aagcagcacg agttttctca tgctaccggt gagctcactg
    21061 ctttgctgtc ggcaataaaa ctgggcgcca agattatcca tcgcgatatc aacaaagcag
    21121 gactggttga tatcctgggt gccagcggtg ctgagaacgt gcagggcgag gttcagcaga
    21181 aactcgacct gttcgctaat gagaaactga aagccgcact gaaagcacgc gatatcgttg
    21241 cgggcattgc ctctgaagaa gaagatgaga ttgtcgtctt tgaaggctgt gaacacgcaa
    21301 aatacgtggt gctgatggac ccactggatg gctcgtccaa catcgatgtt aacgtctctg
    21361 tcggtaccat tttctccatc taccgccgcg ttacgcctgt tggcacgccg gtaacggaag
    21421 aagatttcct ccagcctggt aacaaacagg ttgcggcagg ttacgtggta tacggctcct
    21481 ctactatgct ggtttacacc accggatgcg gtgttcacgc ctttacttat gatccttcgc
    21541 tgggtgtttt ctgcctgtgc caggaacgga tgcgcttccc ggagaaaggc aaaacctact
    21601 ccatcaacga aggaaactac attaagtttc cgaacggggt gaagaagtac attaaattct
    21661 gccaggaaga agataaatcc accaaccgcc cgtatacctc acgttatatc ggttcactgg
    21721 tcgcggattt ccaccgtaac ctgctgaaag gcggtattta tctctaccca agcaccgcca
    21781 gccacccgga cggtaaactg cgtttactgt atgagtgcaa cccgatggca ttcctggcgg
    21841 aacaagcagg cggtaaagcg agcgatggca aagagcgtat tctggatatc atcccggaaa
    21901 ccctgcacca gcgccgttca ttctttgtcg gcaacgacca tatggttgaa gatgtcgaac
    21961 gctttatccg tgagttcccg gacgcgtaat ctcctgcgcc tctccacacg gagaggcgtt
    22021 tttatcgctg cgcaatgttg actcttgtca caggcgaact ctgacgattc tcccacagca
    22081 ccgttaatcc acgctgtaat gctataaaaa taaacaacaa aataccgatg gcgattttcg
    22141 tccaccatga gctcagcgtg ccatcaaagt ttatgtaagt ctgaatcagt ccctgaatcg
    22201 ccacgccaaa aagcgtccct aataccgttc caacgccacc actcaaaagc gtaccgccaa
    22261 ttaccactga ggcgatagca tccagttcca cacctacgcc cgccagcgca tatccggcct
    22321 gggtataaat cgagaagaca atccccgcca gtgtcgccag cccggtagag agcatataaa
    22381 tgcgaatagt ggtgctgcga gtggaaatcc ccatcaggtt cgccgacgtt gcgttgccgc
    22441 caatggcgta cacctgatta ccaaaacgcg tacgatgcgc gaggaatatg ccgataacca
    22501 ccaccgccag catcagcagc cccatcgcac ttaagcgacc gccgccaggg attttccacg
    22561 caaggcttga gagcgtgtca taaacaggat ggtttatcgg gatcgactct tccgaaacga
    22621 gatagctgac gccacgcaaa aagaacattc ctgccaaagt aatgataaac gccgggatct
    22681 tcagggcgtc aatcagaagc cccataaatg cgccgaaggc acagcccatc accaacacca
    22741 gcggaaacgc cagcagcggc gagaggccga aatcgccaat cacttttgcc agaaacacgc
    22801 cggtaaaggc gatcaccgaa ccgacggaga gatcgatccc accggagagg atcacaaagg
    22861 tcatgccaac ggcgattatc ccaagaaagg cattatcggt caggatattg cagatcactc
    22921 ttgtggaagc aaaaccggga aactgggtca gacagtagat ataacccaac acaaagacgc
    22981 cgatggtgat catcagtggc aaattacgtt ttatcacggt tacgtacccc tttaatcaga
    23041 ctgataaagc gttgcgactg gacaatcagc acgcaaagca ccaccaccgc tttcaccacc
    23101 tggttcatct ccggcggaaa tccagaaagc aaaattccgg tgttcattcc ctgaataatc
    23161 agcgccccca ccaccgaaag cagcaggtta aagcgcccgc ccatcagcga tccgccgcca
    23221 atcaccaccg cgagaatggc gtccagctcc agccataacc cggcgttatt ggcatcggca
    23281 ccgcgaatat ccgccgccac gataatgccc gcaatcgccg cacatagccc gctcaacacg
    23341 taggtgagca tgacgatgat tcgcgtgttt accccggcat tttttgccgc ccgaatattg
    23401 ataccaacgg cttcgataaa catccccagt gccgttttgc gggtcaacag ccagaacagg
    23461 agaagcgtca gcaccgcaat aatgactggc gttggcagga acaacagcga tccactgccg
    23521 aaccaggaga gatccggtga gttaaatgtg acgatctgtc cggaggtgat cagttgcgcc
    23581 acaccgcgcc cggcgaccat caggatcagg gtggcgacaa acggctgaat tttgaggatc
    23641 gctaccagta tgccgttcca caatcctgcc aggatgccag tgcccagggc gcttaacaaa
    23701 acaatcggca ggctgaatcc cgcgaccgtc atcgcagccg ttgtggctcc ggcgatagcc
    23761 atcaccgccc ctacggagag atcaatccca ccggtggcga taaccagcgt catgccaatc
    23821 gccagtagcg caacgggggc cgcacggtta agaatgtcga tagggctacc gaacaaacgc
    23881 ccatcctgaa gcaccacctg ccagaaatgc ggggccacca ggctatcgac cagcagcacc
    23941 agcaacagtg ccgccagctg cggcattcct gttggccagc gaaaacgcct tttcggcggc
    24001 gtagtgtccg ggagagattg aggcatcaca ctattctcct tacgccgcaa tggcgttcat
    24061 gatcgccgga acggaaagct ctgccagcgg gatctccgcc acctgtttgc gatcgcgcat
    24121 gataatcacc cggtcggcat agcccaccag ctcttccagt tcagaggaga tcaccagcag
    24181 cgccagaccg tcagcgcaca gcgtttcaat caggcggatg atttcagcgt gggcaccaac
    24241 atcaatgccg cgggttggct cgtcgaggat caaaaattgc ggacgagtca gtagccaacg
    24301 tgaaagcaac actttttgct gattgccgcc agagagaaat tcaatcggtt gttcagttga
    24361 aggtgtgcga atgccaagct ggcggataaa gcgttcggca atctcttgct gttctttgcg
    24421 ggaaatcggt cgtagccagc cgcgctgggc ctggagagcg aggatgatat tttcccgcac
    24481 cgaggcggcg gcgatgatgc catcggtttt cctgtcttcc gggcagaagc cgatacccag
    24541 taccgaagcc tgatgtggcg atcgcagggt ttgctgtttg cctttgatca acgccgtgcc
    24601 gctgtcagca ggtttgatcc cgaagatcac ttcggcggtt tcggtacgtc cggatcccag
    24661 caagcctgcc aggccaacga tctcgccggg gcgtacttcg agatcaaacg gtgcgatcgt
    24721 tccttttttg ccgtaatttt tgaacgcggc aacgggtttg tcgctcagca atgttctccc
    24781 ggcacgctga agcgcgtggg tgtccagttc acgccccagc atcattttta ccagttcgat
    24841 ctgcggtagc tcgcgggttt cccgacagcc cacaaaactg ccgttacgta agacggtgat
    24901 ccgatcgcta acctgataaa cctgatcgag aaaatgagtg acgaagatca ggctgacgcc
    24961 gcgatcgcga agctgacgca tcaggccaaa cagtaactct acttcctgag tgtcgagact
    25021 ggcggtgggt tcatcgagga tcagcacttt ggcggagaga tcgatagccc ggcaaatggc
    25081 gacgatttgc tgcatcgcga ctgaaaagcg gttgagcggt tcgcgcacgt cgagggagaa
    25141 accgtaagat gccatcagtt cggtggcgcg cttttccatc tctttacggc gtagaaggcc
    25201 gaaacgtttg ggttcgcgac ctatgaatag attatcagcg accgacatat tgggtagcag
    25261 gttgacttcc tgatagacgg tgccgatgcc gagttgttgt gcgtgggcgg tgtttttcgg
    25321 tgagatagcc tggccttcca gccagatagt gccgcgatcg gcgtggtata caccggttaa
    25381 tgctttgatt agcgtcgatt ttcccgcccc gttttcaccg agcagcgcca tgatttcgcc
    25441 acggcgcagg ctgaaatcaa cgttgtctaa cgctttgaca ccggggaaaa atttacttaa
    25501 tccttcggtg cggaggattt cctggtgttg gtcggtcgtc atggtttccc ccggtgtaaa
    25561 gcgtgctccc agtaggcatg ataagacgcg gtaagcgtcg catcaggctt cagcgcacaa
    25621 ccgccggatg cggcgcgagc gccttatccg gcctacgaat cggcatcagg catagcaacc
    25681 ctcaataccc catatttttc ttcttctcta actcttcttt tgcggtatca ggcagataaa
    25741 gggtggattt ggttaacgtc agcttttcag gcatggtgcc gtctttttta tatttctcca
    25801 gcgcgtcgaa ggcgggacct gccatattcg gcgtcagttc aacactggcg ttcgcttcgc
    25861 catcaatcat cgctttgtag atgtccggca caccgtcgat agagcctgtc aggatatctt
    25921 tgcccggttt caggcccgct tctttaattg cctgaattgc accaatcacc atgtcatcgt
    25981 tatgggcgta aaccatgcag atgtttttgc cgttgttttc cgctttgata aagctctcca
    26041 tcacttcttt gcctttgctg cgggtgaagt cacctgactg cgagcggata attttgatat
    26101 ttggcgcatt cttaatggct tcggcaaagc ctttcttacg gtcaatggcg acgctggccc
    26161 caacggtgcc ctgcagctcc accacgttgc atggtttgcc attcacttct tttaccagcc
    26221 agtcaccaat caacttgcct tcgaggatgt tgtcggcggt gacagtggtc atatagagag
    26281 atttgtcttt cacatcgatg gaacggtcaa gcaagaagac cgggatttcg gcatctttcg
    26341 cctcttttaa taccggctcc caaccggtcg ctaccactgg agcgataaag atcgcatcca
    26401 ccccttgcgc gacgaaggaa cgtaccgctt taatctggtt ttcctgcttt tgctgaccat
    26461 cggcaatttt caacgtaatt ccgcgctttt cggcttcact tttcgccaca ttggtttctg
    26521 cggcgcgcca gccggattcc gacccgacct gcgaaaatcc aacggttaat ggagcggcca
    26581 acgccataga cgacatggct gccgagactg cagagactac aagtaagcgt ttccacatag
    26641 ggttgtcctc gtcggggtaa ttattattgg tgaaaagatg ttcgcgaaaa aactatagac
    26701 aattcgttat gtaacggatt gcgttacatc acacttcaaa aaagtaaata aaacgttaat
    26761 cacaagtttg taatcgcttt catctcacta tgaaaaatgc ggctacggtt atggattttc
    26821 ctgctctgta taccgtctta aaactggcga aaaaggaaaa tgaagacgaa aacaagcgaa
    26881 gacattcggc gcgagttggc tataatactc ggcacttgtt tgccacatat ttttaaagga
    26941 aacagacatg agcttactca acgtccctgc gggtaaagat ctgccggaag acatctacgt
    27001 tgttattgag atcccggcta acgcagatcc aatcaaatac gaaatcgaca aagagagcgg
    27061 cgcactgttc gttgaccgct tcatgtccac cgcgatgttc tatccgtgca actacggtta
    27121 catcaaccac actctgtctc tggacggtga cccggttgac gtactggtcc cgactccgta
    27181 cccgctgcag ccgggttctg tgatccgctg ccgtccggtt ggcgtactga aaatgaccga
    27241 cgaagccggt gaagatgcga aactgattgc tgttccgcac accaagctga gcaaagaata
    27301 cgatcacatt aaagacgtta acgatctgcc agagctgctg aaagcgcaaa tcgctcactt
    27361 cttcgagcac tacaaagacc tcgaaaaagg caagtgggtt aaagttgaag gttgggaaaa
    27421 cgcagaagcc gctaaagctg aaatcgttgc ttccttcgag cgcgccaaga ataaataagt
    27481 tcttctggcg caataaccct gaacaccggg cttcggttag taagggtttt tttatgcccg
    27541 caatttataa actctctact ccactattat tattttcagc agttgaaagg cttgaacggt
    27601 aagaacaagc taaactcgac caccattttg ctgttcatag ccacttgctg gaagttagcc
    27661 ggcctcactc atactcaccg ttcaaagcgt gtccccgttt tacccctgta catttgttaa
    27721 cgccagcaga gttttttatg cgattgcaga tatggaaagc ggcccggaga gaaaagcaaa
    27781 agatgggaaa gttacaaaaa aaatcgcgag atacatcgca taaaacaacg ccaccttttc
    27841 ggtggcgttt ttatcgccca cgcaccacca gtgcctggtc cagctcgcga tacgcttcaa
    27901 ccagtttctc cagtgaaacg cgacttaaac cgctgggatt tggcagcacc caaatctgcg
    27961 tcgaaccaat ggtgagagtt tgtttccccc actgtgcacc gcgctggctg aatccctgtt
    28021 catatgcttg tttgcccaga atcgccaacg cctgcggctg ataatcttca attttttcaa
    28081 tcagcttacg cccgcctgcg tgtagctcct gctttgaaac ttcattggct tgcaccgttg
    28141 gacggtctac cagtttggtg acgccacaac gataatccag cagatgctgt gcctcctgcg
    28201 gcttcaactg acggtcggta aacccggcct gatatatcac cttccagaag cgatttgccg
    28261 gatgagcaaa gggaaaacca gtcccggcgg atgaaagccc agggttgata ccgcaaaaca
    28321 cgacccgtaa ccctggagcc aaaatatcct caaccatatt tactcctttc taacatcacc
    28381 aggaaaagta taacggattg aaaatacatt gtttataaaa acagcaggcg cgcggtaatg
    28441 gctggattgc gacacggagt tactttataa tccgctacca tggcccctta gctcagtggt
    28501 tagagcaggc gactcataat cgcttggtcg ttggttcaaa cccaacaggg gccaccatat
    28561 acaacaaagg gttagctagt aaatcagcta accctttttc ttttgccttt gaattgggtc
    28621 aggtaatggg tcaggtaagc tttttctcca tctgactcag atggctgata gttgccccgg
    28681 ataatgcttc gcaatgatgt catttagccg atcaaccagt tctggttttc tgaacgtgat
    28741 atgcgcggag cccttcttaa agtatctgat actgaacatc tcatcttcat agtcctcttt
    28801 accctgaaca gactggatgt gatcatccag atgaatggtg atgttttccc ggttgtcggg
    28861 gatcggtttg ccgctgaaca ggtgtagcat tctttccaga tcggcaagtc gatcggcctg
    28921 ttgcccgctg ttcagatgaa atccccacct gtcccacctc accagattgt tgacgataat
    28981 tttactgcca aatttacagg gtgagttggt tttgtaatcc cagctcagtc ccctgaagac
    29041 gttgatcacc cctcgctcaa acacctcatt cttattctgg tgcagttgtt caaatgtgct
    29101 cagaatgttc gcttcactga tttccggaaa attatcgtac tccagtgacc ggtaccatgt
    29161 atcacgggcc tgtgcatcca ttaaggaaag catccctgaa cgttgcatca ggtcacgcca
    29221 gatttctcga tcaagattgc gggtgatggc tttcattgcc gtttcaggtt tttccatcag
    29281 ccagcaaccg cagcgaaaat cctgtttcat tgcccagtcc agtgcggttt taccgccaat
    29341 actttctgtc attgctgata tttcagccag ttgccggata agtgtgtcga tttgcagcag
    29401 tgtactcttt cgtacagcca gaatgtggcg gatactggtt gagcagataa cgtccgtatg
    29461 cccggtgacc agttccggac gggagattgt gatgtctgac attatcggtt tccctttggg
    29521 tttataaacg aaaacgccag ccctgttcag gctgacgtca gggtaatgaa gtctggtgag
    29581 taagactcac cggttctgtt gcagaaactg cagttggcgc agggtacgga tatcttccct
    29641 ggcctgttca atgcagggca gcagtgctgc cgaatccgtt ttatcctcat cgttgtcaaa
    29701 gataattccc gattcgcagt cgatattgtc ctgcagccat gcaatcagaa tatccagcgc
    29761 tgtttccgtg gttaatgatt tcatgttgtt tctggttacc agtcgaaagt ggggaaaccc
    29821 ggcagacatc cggcactggc atccagatga atgagactga cgccataacg tcggatgagt
    29881 gtgacaacca gacggcggaa cgtttttgac agccccaggc gtttaagccg cagaaccggg
    29941 tatgaccacg catcggtacg taacagatat ccgctacctg tgaaatgaat ccattctgat
    30001 tcaccaacgt cactggtctg atgcgacagc gagtacagcc aggcgttatc cttttccgtg
    30061 atatgtgcgg tactgcagcg tatgccggca agggttgcaa acggtggtag tgtgcaggtt
    30121 gactgttggt cggattcctc caccacgcgg agtgaataac cgttttcagc gaccttgtta
    30181 atcagttcag tgagattaac accatcgacg tcaacgacaa tttgccccat gttcagtgcc
    30241 tgcacattaa ttctgtcggc ttccagcatc agggaaagtt tcatcgtttc tccggatgct
    30301 tacccagggt aatgttattt accgttctgt aattgtcgcg ggtcatcaga cctgttgccc
    30361 ggcgtgcccg gagaatatca atgctgttga ttaactgcga cgaagttcct gcgctgaatc
    30421 ccggctggtc ggtacgcacc agcgcgtatt tttccacgag aaaattcacc gcatcacaca
    30481 gtgaaatgcc tgcctcaata tgctgctcaa tcacacgttc atcagcgaac ggtgtgtcat
    30541 tcagcgtaag gccatagtgc tggtccagca gtcgggtgag cagtgtctgc cagatttcaa
    30601 caggagatgg gcgtgaactg gccgactgcc cgggtaatac aggtaatgtt ttcatactga
    30661 agattttcct gatatgcaaa gatgaaaaat gggaaagtgg tgtggtgaaa acaccaggcc
    30721 ggagcagagg gttattctgg agagttactt tttcgtttcg ggcgtcggat aaacagccat
    30781 ataaacgtag ccacaactgc cgagggtgtc ggcttcgcag gtcagccctc tggcatacag
    30841 cgtgacggta tgctgatggc ggggattcag ttcaccgctg gtgagcatga gttccagttg
    30901 tttcatcagc agcggaaagg cctggtccag gtggtacgca tctgcatcgc tgaacaggcc
    30961 tctgatacca gcgcggtcgg caaggtaatg caaccggtta ccctcctgca ccagacgggc
    31021 gccgaaacag ggcgtcacgg tgcagggcag cccccaccag gggcggtcgt gattgccgtc
    31081 gggaagtgtg tctgacacga taaaatcctc acagaaaatc ggttaagaat ggtccggtgg
    31141 tggcgataat tctgctcatc agaatgccct gactcagttc agggtgacgc tcatcagccg
    31201 gacatacggg ccaaaactgt ccttacggcg ttcagcaaac acggccagca caccgggaat
    31261 atcctgtact tcacgaccgg tatacgcccc ggcactgccg tgccagcggt acttaccggt
    31321 gcagaacgga aaaagacggg aggccggatg ctgttggtga atacgcatgg cttcaccacg
    31381 ggtgatgatt ttcatgatgt gatacctctg aagacagaag ataaaagtga aaacaggtat
    31441 gatgtggttg tgacggtgac gggttaaagc agaccgtgtt cggcaaagga gaaaacctgg
    31501 ctgccaccga ctatcagatg gtccggcacc cggatatcca ccaggcccag tgcctgtacc
    31561 agacgttccg taataagccg gtctgcctta ctgggtgtga cttcaccgga cgggtgattg
    31621 tgtgccagta ccacggcagc ggcattgtgg tacagggcgc gtttaatcac ttcccgggga
    31681 tggacttccg tgcggttgat ggtgccggtg aagagggttt caccggcaat caactgattc
    31741 tggttgttca gatacagcac ccggaattct tcacgctcca gtcccgccat gttcagaatc
    31801 agccattccc gtgccgctcg ggtggaggtg aaggccacgc cgggttcatg aagatggcgg
    31861 tccagggttt tcagggcccg cagaatgaga ctgcgctcgc cgggcgtcat ctctccgggc
    31921 agaaaggaaa gttgttgcat tgtgcttctc tccattcagt cgatgatgcg cataatggcg
    31981 ctgcattccg gatgctgcag ggcgtaatcc cgcaaccggt aataatggac cgtcatggca
    32041 taacactccg tacgacaggc atgatgactg tacgtcatca gacaggcagc aatgccggcg
    32101 gcttccgggc tcatttcagc gcggttaccg ttcatggcat tgaacagtac ccagttttcg
    32161 ccatcatcgt catccggttc gggggccata aatgcgccac cgttgttcag tgtgtacaga
    32221 ttccagatac caccgcagta gtcttcgcaa agacggtcca tccagccgaa gatacggggc
    32281 tccagggtca cccactgtgg aatgaggcca aaatgctgcg gccagaagct gatgcgctgt
    32341 tcatcaggga ctatggtggc aaccagctga ggctggtcac tccctgatgc agttgttacg
    32401 gaaacagaag gtgtggtgga gctatgcgaa aaggttgtca tgaaatgatt ccttataaaa
    32461 agcaaatgaa tgaagaagac tcgggggcag ggacagacgt gagtcaggac ggcgctttca
    32521 ggtaaacggt atcagcgcat gctctccagc agcgtttccg ccatcaccca caatgcgcga
    32581 ttgagcttga tgtcggtgtc gatactgtga atggcacggg tatggatacg ttttcctttt
    32641 gcactacggc cggaaatccc gcctttcagc atattctcct ggatggtctg atatgcgctc
    32701 cacaggtcct taccgtaatc ctcccggcgt cgtggtgtca gaatgtcggc agtggtgacg
    32761 ggttgatgtt catcaccata acggtaagtc agcgccgcct gtgccagtgc ctgacgtgcc
    32821 ggtggcggca gaaccagaga ctgcatggca tcacgctttt cctcaatccg gtcaaaaacg
    32881 cccactacct cgtaagcccc ttcaatgact ctgtccacca catttccccg gtggggcaca
    32941 cggacttccc ccagagactg accgcagacg cagccgttct ggcagacgaa tctgaagtaa
    33001 cccggcagca tctggtagct ggaggtaccg tcatgggagt tgagcagaat aatttcaggc
    33061 acatgctgac cggttatctg tccggcccgc cgcagacgca gcatgtgttt ggtatattcc
    33121 cggcggcccg ggtcacgtac gcgggtctgg caggcgaaga atggctgaaa gccttcccgc
    33181 tgcaggcttt ccagcacggt gatggtggga atccaggtat agttctcgct tctggatgtg
    33241 tgtttctctt ccccgaatac gctggggacg acctgcatca gttcttcacg agttaagggg
    33301 cggtcacggc gtatctggtt cgcataacca aaacgactgg ctaatcgcat gatttattcc
    33361 ttatgaatga gttagcggta taaatgaaaa agccgcgtct cccggagaag acgcggcctg
    33421 atgggggaaa tgaatgacgt ttattgtctg agaagccctt cactggcgag ctgcgtattt
    33481 ttatggaagg agatcaccca ataaccccat cgggatcggg taataaccgg tcagactgtg
    33541 atcgattcag atgccatttg gcagcaaaag agccttcggc aataaacgtg ctgtacaacc
    33601 cggcatatct gttgctccct gaa
//
LOCUS       DAZGQL010000057        33285 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.057, whole genome shotgun sequence.
ACCESSION   DAZGQL010000057 DAZGQL010000000
VERSION     DAZGQL010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33285
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.057"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(370..2058)
                     /gene="betA"
                     /locus_tag="ACHKIR_002936"
     CDS             complement(370..2058)
                     /gene="betA"
                     /locus_tag="ACHKIR_002936"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008812 - choline dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HFO9990641.1"
                     /translation="MQFDYIIIGAGSAGNVLATRLTEDPNTTVLLLEAGGPDYRFDFR
                     TQMPAALAFPLQGKRYNWAYETEPEPFMNNRRMECGRGKGLGGSSLINGMCYIRGNAL
                     DLDNWAKEPGLENWSYLDCLPYYRKAETRDVGENDYHGGDGPVSVTTSKPGVNPLFEA
                     MIEAGVQAGYPRTDDLNGYQQEGFGPMDRTVTPQGRRASTARGYLDQAKSRPNLTIRT
                     HAMTDHIIFDGKRAVGVEWLEGDSTIPTRAAANKEVLLCAGAIASPQILQRSGVGNAE
                     LLAEFDIPLVHDLPGVGENLQDHLEMYLQYECKESVSLYPALQWWNQPKIGAEWLFGG
                     TGVGASNHFEAGGFIRSREEFAWPNIQYHFLPVAINYNGSNAVKEHGFQCHVGSMRSP
                     SRGHVRIKSRDPHQHPAILFNYMSHEQDWQEFRDAIRITREIMHQPALDQYRGREISP
                     GAECQTDEQLDEFVRNHAETAFHPCGTCKMGYDEMAVVDAEGRVHGLEGLRVVDASIM
                     PQIITGNLNATTIMIGEKMADMIRGQEALPRSTARYFVANGMPVRAKKMSRDVN"
     gene            complement(2072..3544)
                     /gene="betB"
                     /locus_tag="ACHKIR_002937"
     CDS             complement(2072..3544)
                     /gene="betB"
                     /locus_tag="ACHKIR_002937"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008802 - betaine-aldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HFO9990642.1"
                     /translation="MSRMAEQQLYIHGGYTSATSGRTFETINPANGNVLATVQAAGRE
                     DVDRAVKSAQQGQKIWAAMTAMERSRILRRAVDILRERNDELAKLETLDTGKAYSETS
                     TVDIVTGADVLEYYAGLIPSLEGSQIPLRETSFVYTRREPLGVVAGIGAWNYPIQIAL
                     WKSAPALAAGNAMIFKPSEVTPLTALKLAEIYSEAGLPDGVFNVLPGVGAETGQYLTE
                     HPGIAKVSFTGGVASGKKVMANSAASSLKEVTMELGGKSPLIVFDDADLDLAADIAMM
                     ANFFSSGQVCTNGTRVFIPAKCKAAFEQKILARVERIRAGDVFDPQTNFGPLVSFPHR
                     DNVLRYIAKGKEEGARVLCGGDVLKGDGFDNGAWVAPTVFTDCRDEMTIVREEIFGPV
                     MSLLTYESEDEVIRRANDTDYGLAAGIVTADLNRAHRVIHQLEAGICWINTWGESPAE
                     MPVGGYKHSGIGRENGVMTLQSYTQVKSIQVEMAKFQSIF"
     gene            complement(3558..4145)
                     /gene="betI"
                     /locus_tag="ACHKIR_002938"
     CDS             complement(3558..4145)
                     /gene="betI"
                     /locus_tag="ACHKIR_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308386.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HFO9990643.1"
                     /translation="MPKLGMQSIRRRQLIDATLEAINEVGMHDATIAQIARRAGVSTG
                     IISHYFRDKNGLLEATMRDITSQLRDAVLNRLHALPQGSAELRLQAIVGGNFDETQVS
                     SAAMKAWLAFWASSMHQPMLYRLQQVSSRRLLSNLVSEFRRELPRQQAQEAGYGLAAL
                     IDGLWLRAALSGKALDKPLAHSLTRHFITQHLPTD"
     gene            4274..6307
                     /gene="betT"
                     /locus_tag="ACHKIR_002939"
     CDS             4274..6307
                     /gene="betT"
                     /locus_tag="ACHKIR_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HFO9990644.1"
                     /translation="MTDLSHSREKDKINPVVFYTSAGLILLFSLTTILFRDFSALWIG
                     RTLDWVSKTFGWYYLLAATLYIVFVVCIACSRFGSVKLGPEQSKPEFSLLSWAAMLFA
                     AGIGIDLMFFSVAEPVTQYMQPPEGAGQTIEAARQAMVWTLFHYGLTGWSMYALMGMA
                     LGYFSYRYNLPLTIRSALYPIFGKRINGPIGHSVDIAAVIGTIFGIATTLGIGVVQLN
                     YGLSVLFDIPDSMAAKAALIALSVIIATISVTSGVDKGIRVLSELNVALALGLILFVL
                     FMGDTSFLLNALVLNVGDYVNRFMGMTLNSFAFDRPVEWMNNWTLFFWAWWVAWSPFV
                     GLFLARISRGRTIRQFVLGTLIIPFTFTLLWLSVFGNSALYEIIHGGAAFAEEAMVHP
                     ERGFYSLLAQYPAFTFSASVATITGLLFYVTSADSGALVLGNFTSQLKDINSDAPGWL
                     RVFWSVAIGLLTLGMLMTNGISALQNTTVIMGLPFSFVIFFVMAGLYKSLKVEDYRRE
                     SANRDTAPRPLGLQDRLSWKKRLSRLMNYPGTRYTKQMMETVCYPAMEEVAQELRLRG
                     AYVELKSLPPEEGQQLGHLDLLVHMGEEQNFVYQIWPQQYSVPGFTYRARSGKSTYYR
                     LETFLLEGSQGNDLMDYSKEQVITDILDQYERHLNFIHLHREAPGHSVMFPDA"
     gene            7184..8272
                     /gene="pdeL"
                     /locus_tag="ACHKIR_002940"
     CDS             7184..8272
                     /gene="pdeL"
                     /locus_tag="ACHKIR_002940"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator/c-di-GMP
                     phosphodiesterase PdeL"
                     /protein_id="HFO9990645.1"
                     /translation="MNSCDFRVFLQEFGTTVHLSLPGSVSEKERLLLKLLMQGMSVTE
                     ISQYRNRSAKTISHQKKQLFEKLGIQSDITFWRDIFFQYNPEIISATGNNSHKYINDN
                     HYHHIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGIIPPDQFI
                     PLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNL
                     VKKLGNDKIKLVLELTERNPIPVTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQA
                     FPVDFIKIDKSFVQMASVDEISGHIVDNIVELARKPGLSIVAEGVETQEQADLMIGKG
                     VHFLQGYLYSPPVPGNKFISEWVMKAGG"
     gene            complement(8314..9246)
                     /gene="yahB"
                     /locus_tag="ACHKIR_002941"
     CDS             complement(8314..9246)
                     /gene="yahB"
                     /locus_tag="ACHKIR_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO9990646.1"
                     /translation="MNSIFTEENLLAFTTAARFGSFSKAAEELGLTTSAISYTIKRME
                     TGLDVVLFTRSTRSIELTESGRYFFRKATDLLNDFHAIKRSIDTISQGIEARVRICIN
                     QLLYTPKHTARLLQVLKKQFPTCQITVTTEVYNGVWDAIINNQANIAIGAPDTLLDGG
                     GIDYTEIGAIRWAFAIAPDHPLAFVPEPIAESQLRLYPNIMVEDTAHTINKKVGWLLH
                     GQESILVPDFNTKCQCQILGEGIGFLPDYMVREAMAQSLLVTRQIHNPRQDSRMLLAT
                     QHSATGQVTQWIKKQFSPNGILTGIYQDLLHREN"
     gene            complement(9338..9835)
                     /gene="yahC"
                     /locus_tag="ACHKIR_002942"
     CDS             complement(9338..9835)
                     /gene="yahC"
                     /locus_tag="ACHKIR_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFO9990647.1"
                     /translation="MNGLTATGVTVGICAGLWQLVSSHVGLSQGWELLGTIGFVAFCS
                     FYAAGGGKSGFIRSLAVNYFGMVWAFFAALAAGWLASVSGISGFWASVIMTVPFSAVV
                     VWQGRFGLLSFIPGGFLGMTLFFASGMNWTVTLLGFLAGNCVGIISEYGGQKLSEATT
                     KRDGY"
     gene            10093..10698
                     /gene="yahD"
                     /locus_tag="ACHKIR_002943"
     CDS             10093..10698
                     /gene="yahD"
                     /locus_tag="ACHKIR_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HFO9990648.1"
                     /translation="MTIKNLPADYLLAAQQGDIDKVKTCLALGVDINTCDRQGKTAIT
                     LASLYQQYACVQALIDAGADINKQDHTCLNPFLISCLNDDLTLLRIILPAKPDLNCVT
                     RFGGVGLTPACEKGHLNIVKELLAHTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIV
                     QLLLEHGASPHMTDKYGKTPLELARERGFEEIAQLLIAAGA"
     gene            10738..11601
                     /gene="yahE"
                     /locus_tag="ACHKIR_002944"
     CDS             10738..11601
                     /gene="yahE"
                     /locus_tag="ACHKIR_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFO9990649.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFDGLFRHGDKVQFDDQGITVGQHLHIEMSRCRRWLSPTLQMAAV
                     NFHLIAWQQWHDIIHQHLGENETLFNYCGDNPFYQALNKELHIKRRAVIQAVNDKQNI
                     ASAVASMMGLGIGLTPSADDYLTGLALILFISGHPAEKYKEEFYLGLQCGRNNTTLLS
                     AITLEAALQQRCRENIHRFIHNIIYDIPGNATQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            11591..13138
                     /gene="fdrA"
                     /locus_tag="ACHKIR_002945"
     CDS             11591..13138
                     /gene="fdrA"
                     /locus_tag="ACHKIR_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFO9990650.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLRNLGLLTPELEQAKNGDLMIVINGKSGADNEQLLVEIEELFNSKAQSGSHEARYA
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIVLISKPPAPAVARKVLERARACRK
                     PVVVCFLGRGETPVDEQGLQFARGTKEAALKAVMLSGMKQEHLDLHTLNQPLIADVRA
                     RMQPQQKYIRGLFCGGTLCDETMFAVMEKHGDVYSNIQPDPEFRLQDINRSVKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            13138..14556
                     /gene="yahG"
                     /locus_tag="ACHKIR_002946"
     CDS             13138..14556
                     /gene="yahG"
                     /locus_tag="ACHKIR_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFO9990651.1"
                     /translation="MSPSLFSQPLNVINVGIAMFSDDLKKQHVEVTQLDWTPPGQGNM
                     QVVQALDNIADSPLADKIAAANQQALERIIQSHPVLIGFDQAINVVPGMTPKTILHAG
                     PPITWEKMCGAMKGAVTGALVFEGLAKDLDEAAELAASGEITFSPCHEHDCVGSMAGV
                     TSASMFMHIVKNKTYGNIAYTNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPILRDA
                     MKIIGEIDLRLMLAQALHMGDECHNRNNAGTTLLIQALTPGIIQAGYSVEQQREVFEF
                     VASSDYFSGPTWMAMCKAAMDAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPA
                     QQVIGPMFAGYKPEDSGLDIGDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSR
                     QMREITLGENPNVTIPLLGFMGVPTAIDITRVGSSGILPVINTAIAHKDAGVGMIGAG
                     IVHPPFACFEKAILGWCERYGV"
     gene            14582..14836
                     /locus_tag="ACHKIR_002947"
                     /pseudo
     CDS             14582..14836
                     /locus_tag="ACHKIR_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            14886..15836
                     /gene="yahI"
                     /locus_tag="ACHKIR_002948"
     CDS             14886..15836
                     /gene="yahI"
                     /locus_tag="ACHKIR_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HFO9990652.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGVFFSESQRDELQKANPDWRFVEDAGRG
                     YRRVVASPEPQRIVEAPAIKALIQQGFVVIGAGGGGIPVVCSEAGDYQSVDAVIDKDL
                     STALLAREIHADILVITTGVEKVCIHFGKPQQQALDRVDIATMTRYMQEGHFPPGSML
                     PKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT"
     gene            15846..17228
                     /gene="yahJ"
                     /locus_tag="ACHKIR_002949"
     CDS             15846..17228
                     /gene="yahJ"
                     /locus_tag="ACHKIR_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFO9990653.1"
                     /translation="MKENNSRREFLSQSGKMVTAAALFGTSVPLAHAAVSGTIHCDAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQNGKIVALRENNQHPDAT
                     LPHYDAGGKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLEQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQAGFECEIVAFP
                     QHGLLLSKSEPLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPAGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDELANRMAAQQISI
                     ASTVQIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDILPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGNVAG"
     gene            17524..17934
                     /locus_tag="ACHKIR_002950"
     CDS             17524..17934
                     /locus_tag="ACHKIR_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFO9990654.1"
                     /translation="MTIKEKFSQKYPHASFCTFGDSAALADHLATLIATGVKTASCGS
                     LAGCIEDNAFPLIGEYKIVENSRGEPVCVIRVIGLHLLRFSDVTAELARKEGEGDLSL
                     EYWRNEHRRFFQAEGCYSPEMDVIFEEYALIDVV"
     gene            18188..19171
                     /locus_tag="ACHKIR_002951"
     CDS             18188..19171
                     /locus_tag="ACHKIR_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO9990655.1"
                     /translation="MNKRFVINMVSSLLLGAALISAPLQAAEKVVVNISKVDGMPWFN
                     RMGEGVVEAGKAFGVNASQVGPSSTDAPQQVKIIEDLIARKVNAITIVPNDANVLEPV
                     FKKARDAGIVVLTNESPGQPSANWDIEIIDNEKFAAEYVEHMAKRMGGKGGYVIYVGS
                     LTVPQHNLWADLLVKYQKEHYPDMHEVTRRMPVAESVDDSRRTTLDLMKTYPDLKAVV
                     SFGSNGPIGAGRAVKEKRAKNKVAVYGMMIPSQAASLIKSGDITEGITYDPASAGYAL
                     AAVASTLLKGEEIKPGLEMQNLGKADVDMDKRIIRFHKVLLVNKDNIDSLY"
     gene            19220..20704
                     /locus_tag="ACHKIR_002952"
     CDS             19220..20704
                     /locus_tag="ACHKIR_002952"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFO9990656.1"
                     /translation="METFLSLRHINKTFHATRALRDVSLDFMSGEVHCLAGQNGCGKS
                     TLIKIMSGVYRPDEGAEITLGGKNWSKLTPAASVAQGIQVIYQDLSLFPNLSVWENIA
                     VNHYHHGLFVNRRRLREVAQAAMTSINVTLPLDTLVSELSIARCQLVAICRALAQDAR
                     LIVMDEPTASLTHQEVQGLLQVVHQLRERGICVVFVSHRLEEVMEVSDRISVLKDGEL
                     VGTFPAAEMTTKQLSFLMTGQEFEYQVRELWQGKSSTPVLEVRNLSRHGEYQNINLRV
                     EAGEVVSIVGLLGAGRTELCLSLFGMTRPDAGEILINGQPVTLHSNQDAIRHGIGYVS
                     EDRMSRGLVMAQSIEDNIISTVFHKVKDRFGFLSEAKVRDLVDRLIKALTIKVSDPHL
                     PVNTLSGGNAQRVSIAKWLAIGPRLLILDSPTVGVDIANKAGIYGIISDLAAHGIAVL
                     MICDEIEEAWYQSHRILVMQKGQITHSFLPDSSSQARIAEVVNG"
     gene            20697..21668
                     /locus_tag="ACHKIR_002953"
     CDS             20697..21668
                     /locus_tag="ACHKIR_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO9990657.1"
                     /translation="MAELKKRHEFWLALLIVVLFVGLAWRSDEFLTFGNLYDLANNYA
                     MLTILACGLFVVLISGGIDISFPAMTIIAQYGMVLLLQKMGGNFAVAFALAGGIGILL
                     GLINALLVNRLRVPSIIITISTLNIFYGLLLWLSKGVWLYDFPPWFEQGVMLFKYTDA
                     DGYDYGLGLPLIAMITVVLLTAFIMNFTSVGRKIYALGGNRESASRIGFSVLKLQLFV
                     YGYMGLMSGAAGVVQSWTVMTVAPDSLLGYELTVLAAVVLGGTSLLGGRGTLTGTLLG
                     VVLLAVMQNGLNLLGVSSYWQTLITGIIIVASISATAWSQHQNRSLL"
     gene            21665..22621
                     /locus_tag="ACHKIR_002954"
     CDS             21665..22621
                     /locus_tag="ACHKIR_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO9990658.1"
                     /translation="MKKSWRNNVEFYLIGLLVLTVAAFSITMPEIFWSISNFQSVASQ
                     MPVLGILALAMAVTMLCGGINLSIIATANACSLVMAWVATQYPPGIATVVATLLAGAG
                     AAVIIGLCNGVLIAGIRVSPILATLGMMTLLKGVNILVTGGSAIANYPSWVLWLNHAQ
                     WFGIPLPMWLFTAVALGLWILLEKTPLGKSIYLIGSNERATLYSGINTRRVLIWVYVI
                     SALLCAVAAFLMMSKLNSAKASYGESYLLVSILAAVLGGVNPDGGSGRIIGMVLALFL
                     LQIIESGFNILGISPYLTMALWGTLLLCFIQARGMLGLDRVV"
     gene            22708..23757
                     /gene="yahK"
                     /locus_tag="ACHKIR_002955"
     CDS             22708..23757
                     /gene="yahK"
                     /locus_tag="ACHKIR_002955"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HFO9990659.1"
                     /translation="MKIKAVGAYSAKQPLEPMDITRREPGPHDVKIEIAYCGVCHSDI
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKYAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMTGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEHLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSELKREAAKALG
                     ADEVVNSRNADEMAAHLKSFDFILNTVAAPHNLDDFTTLLKRDGTMTLVGAPATPHKS
                     PEVFNLIMKRRAIAGSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
     gene            24379..24624
                     /gene="yahM"
                     /locus_tag="ACHKIR_002956"
     CDS             24379..24624
                     /gene="yahM"
                     /locus_tag="ACHKIR_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahM"
                     /protein_id="HFO9990660.1"
                     /translation="MAVQLFKTLLNQIPLLSFLQSGTLPLFGYSGRVRPMKKAHTGES
                     GLKWEAKDSSKLIGNKDHALRGLSSPMVGIRQIRLIT"
     gene            complement(24641..25312)
                     /gene="yahN"
                     /locus_tag="ACHKIR_002957"
     CDS             complement(24641..25312)
                     /gene="yahN"
                     /locus_tag="ACHKIR_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HFO9990661.1"
                     /translation="MMKLLHLFMVEITMDPLHAVYLTVGLFVITFFNPGANLFVVVQT
                     SLASGRRAGVLTGLGVALGDAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAW
                     CSMRRQSTPQMSTLQQPISAPWYIFFRRGLITDLSNPQTVLFFISIFSVTLNAETPTW
                     ARLMAWAGIVLASVIWRVFLSQAFSLPAVRRAYGRMQRVASRVIGAIIGVFALRLIYE
                     GVTQR"
     gene            25459..25734
                     /gene="yahO"
                     /locus_tag="ACHKIR_002958"
     CDS             25459..25734
                     /gene="yahO"
                     /locus_tag="ACHKIR_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein YahO"
                     /protein_id="HFO9990662.1"
                     /translation="MKIISKMLVGALAFAVTNVYAAELMTKAEFEKVASQYEKIGDIS
                     TSNEMSTADAKEDLIKKADEKGADVLVLTSGQTDNKIHGTANIYKKK"
     gene            complement(25835..27421)
                     /gene="prpR"
                     /locus_tag="ACHKIR_002959"
     CDS             complement(25835..27421)
                     /gene="prpR"
                     /locus_tag="ACHKIR_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate catabolism operon regulatory protein
                     PrpR"
                     /protein_id="HFO9990663.1"
                     /translation="MAHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQL
                     GFEKAVTYIRKKLANERCDAIIAAGSNGAYLKSRLSVPVILIKPSGYDVLQALAKAGK
                     LTSSIGVVTYQETIPALVAFQKTFNLRLDQRSYITEEDARGQINELKANGTEAVVGAG
                     LITDLAEEAGMTGIFIYSAATVRQAFSDALDMTRMSLRHNTHDATRNALRTRYVLGDM
                     LGQSPQMEQVRQTILLYARSSAAVLIEGETGTGKELAAQAIHREYFARHDARQGKKSH
                     PFVAVNCGAIAESLLEAELFGHEEGAFTGSRRGGRAGLFEIAHGGTLFLDEIGEMPLP
                     LQTRLLRVLEEKEVTRVGGHQPVPVDVRVISATHCNLEEDMRQGQFRRDLFYRLSILR
                     LQLPPLRERVADILPLAESFLKVSLAALSAPFAAALRQGLQESETMLVHYDWPGNIRE
                     LRNMMERLALFLSVESTPDLTPQFLQLLLPELARESAKTPIPGLLTAQQALEKFNGDK
                     TAAANYLGISRTTFWRRLKS"
     gene            27660..28550
                     /gene="prpB"
                     /locus_tag="ACHKIR_002960"
     CDS             27660..28550
                     /gene="prpB"
                     /locus_tag="ACHKIR_002960"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HFO9990664.1"
                     /translation="MYLHSPGKAFRAALSKENPLQIVGTINANHALLAQRAGYQAIYL
                     SGGGVAAGSLGLPDLGISTLDDVLTDIRRITDVCSLPLLVDADIGFGSSAFNVARTVK
                     SMIKAGAAGLHIEDQVGAKRCGHRPNKAIVSKEEMVDRIRAAVDAKTDPDFVIMARTD
                     ALAVEGLDAAIERAQAYVEAGAEMLFPEAITELAMYRQFADAVQVPILANITEFGATP
                     LFTTDELRSAHVAMALYPLSAFRAMNRAAEHVYNVLRQEGTQKSVIDTMQTRNELYES
                     INYYQYEEKLDDLFARGQVK"
     gene            28710..29879
                     /gene="prpC"
                     /locus_tag="ACHKIR_002961"
     CDS             28710..29879
                     /gene="prpC"
                     /locus_tag="ACHKIR_002961"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HFO9990665.1"
                     /translation="MSDTTILQNSTHVIKPKKSVALSGVPAGNTALCTVGKSGNDLHY
                     RGYDILDLAEHCEFEEVAHLLIHGKLPTRDELAAYKTKLKALRGLPANVRTVLEALPA
                     ASHPMDVMRTGVSALGCTLPEKEGHTVSGARDIADKLLASLSSILLYWYHYSHNGERI
                     QPETDDDSIGGHFLHLLHGEKPSQSWEKAMHISLVLYAEHEFNASTFTSRVIAGTGSD
                     MYSAIIGAIGALRGPKHGGANEVSLEIQQRYETPDEAEADIRKRVENKEVVIGFGHPV
                     YTIADPRHQVIKRVAKQLSQEGGSLKMYNIADRLETVMWESKKMFPNLDWFSAVSYNM
                     MGVPTEMFTPLFVIARVTGWAAHIIEQRQDNKIIRPSANYVGPEDRQFVALDKRQ"
     gene            29913..31364
                     /gene="prpD"
                     /locus_tag="ACHKIR_002962"
     CDS             29913..31364
                     /gene="prpD"
                     /locus_tag="ACHKIR_002962"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047547 - 2-methylcitrate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate dehydratase"
                     /protein_id="HFO9990666.1"
                     /translation="MSAQINNIRPEFDREIVDIVDYVMNYEISSKVAYDTAHYCLLDT
                     LGCGLEALEYPACKKLLGPIVPGTVVPNGVRVPGTQFQLDPVQAAFNIGAMIRWLDFN
                     DTWLAAEWGHPSDNLGGILATADWLSRNAVASGKAPLTMKQVLTAMIKAHEIQGCIAL
                     ENSFNRVGLDHVLLVKVASTAVVAEMLGLTREEILNAVSLAWVDGQSLRTYRHAPNTG
                     TRKSWAAGDATSRAVRLALMAKTGEMGYPSALTAPVWGFYDVSFKGESFRFQRPYGSY
                     VMENVLFKISFPAEFHSQTAVEAAMTLYEQMQAAGKTAADIEKVTIRTHEACIRIIDK
                     KGPLNNPADRDHCIQYMVAIPLLFGRLTAADYEDNVAQDKRIDALREKINCFEDPAFT
                     ADYHDPEKRAIANAITLEFTDGTRFEEVVVEYPIGHARRRQDGIPKLVDKFKINLARQ
                     FPTRQQQRILEVSLDRTRLEQMPVNEYLDLYVI"
     gene            31404..>33285
                     /gene="prpE"
                     /locus_tag="ACHKIR_002963"
     CDS             31404..>33285
                     /gene="prpE"
                     /locus_tag="ACHKIR_002963"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050218 - propionate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HFO9990667.1"
                     /translation="MSFSEFYQRSINEPEKFWAEQARRIDWQTPFTQTLDHSNPPFAR
                     WFCEGRTNLCHNAIDRWLEKQPEALALIAVSSETEEERTFTFRQLHDEVNAVASMLRS
                     LGVQRGDRVLVYMPMIAEAHITLLACARIGAIHSVVFGGFASHSVATRIDDAKPVLIV
                     SADAGARGGKIIPYKKLLDDAISQAQHQPRHVLLVDRGLAKMARVSGRDVDFASLRHQ
                     HIGARVPVAWLESNETSCILYTSGTTGKPKGVQRDVGGYAVALATSMDTIFGGKAGGV
                     FFCASDIGWVVGHSYIVYAPLLAGMATIVYEGLPTWPDCGVWWKIVEKYQVSRMFSAP
                     TAIRVLKKFPTAEIRKHDLSSLEVLYLAGEPLDEPTASWVSNTLDVPVIDNYWQTESG
                     WPIMAIARGLDDRPTRLGSPGVPMYGYNVQLLNEVTGEPCGVNEKGMLVVEGPLPPGC
                     IQTIWGDDDRFVKTYWSLFSRPVYATFDWGIRDADGYHFILGRTDDVINVAGHRLGTR
                     EIEESISSHPGVAEVAVVGVKDALKGQVAVAFVIPKESDSLEDREVAHSQEKAIMALV
                     DSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQAICEGRDPGDLTTIDDPASLDQIRQ
                     AME"
BASE COUNT         7936 a         8314 c         9015 g         8020 t
ORIGIN      
        1 ccacaagtcc ccttttcgtg catgataacc gaataaattg acttttttag cgtagacctg
       61 atattactcc acgctttggg ataaatacgc ccggcttacg cagttgatcg ctgaccactg
      121 ctaaccggga gtgggaaaga acaagtaacc ctagccagga gcactggttg cgtgattctt
      181 aagctgagtg gcattacact tgtggtttat cagcgtatcc atagcaccat catccgccag
      241 attgagcgaa tcggcttaaa gtttgatggc gggactgcgg atatgaagca tgagagttac
      301 ctcagtgttt tatataagga ttcggtcctc atagtagaac ggtaactctc aatctgatag
      361 gttcctgagt tagttcacat cacgactcat ttttttcgct ctcaccggca tcccatttgc
      421 cacaaaatat cttgccgtgc tcctcggcaa cgcttcttgc ccgcgaatca tatccgccat
      481 tttctcgcca atcataatcg tcgtagcgtt caaattgccg gtgataattt gcggcataat
      541 tgacgcatcc accacgcgca ggccttccag cccgtgtacg cggccttcgg cgtcaaccac
      601 tgccatctcg tcataaccca ttttgcaggt accgcacgga tgaaacgcgg tttcggcgtg
      661 gttacgcacg aactcatcga gctgctcatc cgtctgacat tccgcgccgg ggctgatttc
      721 gcggccacga tactgatcca gcgccggttg atgcatgatc tcccgggtga tgcgaattgc
      781 gtcgcggaac tcctgccagt cctgctcgtg cgacatgtag ttaaacagaa tcgctggatg
      841 ctggtgcggg tcgcgggatt taatccgcac atgcccacgg cttggcgagc gcatcgatcc
      901 gacgtggcac tggaagccgt gctctttcac tgcgttcgag ccgttatagt taatcgccac
      961 tggcaggaaa tggtactgaa tattcggcca cgcaaattcc tcacggctgc gaataaatcc
     1021 gcctgcttca aagtggttgc tggcaccaac gccggtgccg ccaaacagcc actccgcacc
     1081 gattttcggc tgattccacc actgcagggc agggtagagg gaaaccgatt ctttgcactc
     1141 atattgcaga tacatctcca ggtgatcctg aagattttcg ccgacgccgg gtaaatcatg
     1201 caccagcgga atatcaaact ccgccagcag ttcagcgttg ccgacgccgg agcgttgcag
     1261 gatctgcggt gaagcaatcg cgcctgcaca taacagcact tctttattcg ccgccgcgcg
     1321 ggtcggaatg gtgctgtcgc cttccagcca ctcgacgccc accgcgcgtt tgccgtcaaa
     1381 aatgatgtga tcggtcatgg cgtgagtacg aatggttagg ttagggcgcg atttggcctg
     1441 atcgagatag ccacgcgcgg tgctggcgcg acggccctgc ggcgtgacgg tgcgatccat
     1501 cgggccaaaa ccttcctgct gatagccgtt gagatcgtcc gtgcgcgggt agcccgcctg
     1561 cacgcccgct tcaatcatcg cttcaaacag cggattgacg ccgggtttgg aggtggtgac
     1621 gctcaccggg ccatcaccgc cgtggtagtc gttctcgccc acatcgcgag tctcggcctt
     1681 gcgatagtag ggcagacagt cgaggtagct ccagttctcc agaccgggtt ctttcgccca
     1741 gttatcgaga tccagcgcgt taccacggat gtaacacatg ccgttgatca gtgatgaccc
     1801 gccaagcccc ttaccgcgtc cgcactccat gcgacggttg ttcataaacg gttccggttc
     1861 cgtctcatag gcccagttat agcgtttacc ctgtaacggg aacgccagcg cggcgggcat
     1921 ctgggtgcgg aagtcaaagc gatagtccgg gccgcctgct tcaagcagca gcacggttgt
     1981 gttcggatct tcagtcagac gggtagcgag aacgttgcca gctgagccgg caccaataat
     2041 gatgtagtca aattgcaaat aaacctcctg gttagaatat ggactggaat ttagccatct
     2101 caacctggat ggacttcacc tgggtgtaac tctggagcgt catcacgccg ttctcgcgac
     2161 caatgccgga gtgtttgtag ccgccaacgg gcatctctgc aggggattcg ccccaggtgt
     2221 tgatccagca aatacccgct tccagctgat gaatgacgcg atgcgcgcgg ttcaggtccg
     2281 ccgtcacgat gcccgccgcc aggccgtagt cggtatcatt ggcgcggcga atcacttcgt
     2341 cctcagattc ataagtgaga agcgacatca ccggaccgaa gatctcttcg cgcacgatgg
     2401 tcatttcgtc gcggcaatcg gtgaacacgg tcggtgcaac ccatgcgccg ttatcgaagc
     2461 catcgccttt cagtacatcg ccgccgcaca gtacgcgcgc gccttcctct ttgcctttgg
     2521 cgatgtaacg cagcacgtta tcgcgatgcg ggaagctgac cagcgggcca aagttagttt
     2581 gcggatcgaa aacgtcgccc gcgcgaatgc gctcaacgcg cgccagaatc ttttgttcaa
     2641 atgcggcttt gcatttcgcc ggaataaaca cgcgggtgcc gttggtacac acctgaccgg
     2701 agctgaagaa gttcgccatc atggcgatat cagcggcgag atcgagatcc gcatcgtcga
     2761 aaacgatcag cggtgattta ccgcccagct ccatggtcac ttctttcagg gaggaagccg
     2821 ccgagttagc catcactttt ttgccgctgg cgacaccgcc ggtaaatgac actttggcaa
     2881 tgcccggatg ctcggtcaga tactgcccgg tttccgcacc tacgcccggc agcacgttaa
     2941 atacgccgtc cggcaggccc gcttcgctgt aaatttcggc cagctttaac gcggtaagcg
     3001 gggtgacttc gctcggtttg aaaatcatcg cgttgcctgc cgccagcgcc ggggcggatt
     3061 tccacaaggc aatctggatc gggtagttcc atgcgccaat ccctgccact acgcccagcg
     3121 gttcgcggcg ggtataaaca aatgacgtct cacgcaatgg gatctggctg ccttccagcg
     3181 acgggatcag cccggcgtag tactccagca cgtccgcacc ggtaacgata tcgacggttg
     3241 aggtttccga atacgctttt ccggtgtcga gggtttccag ttttgcgagt tcgtcattgc
     3301 gttcacgcag aatatcaacg gcccgacgca gaatacgcga gcgctccata gcggtcatcg
     3361 ccgcccagat tttttgccct tgttgggcac tcttcacggc gcgatcgaca tcctcacgcc
     3421 cggcggcctg cacggtcgcc agcacgttac cgttggccgg gttaatggtc tcgaaggtgc
     3481 gaccgctggt ggcggaggta taaccaccat gtatataaag ctgctgttct gccattcggg
     3541 acatcacgtt tcctcggtta atcggtgggt aaatgctgag taataaagtg acgggtcagc
     3601 gagtgtgcga gaggtttatc cagcgctttg ccgctcagtg ccgcgcgcag ccataatccg
     3661 tcaatcagcg cggcaaggcc gtagcccgct tcctgtgcct gttggcgcgg taactcgcgg
     3721 cgaaattcgc tcaccagatt cgacaataag cggcggctac tgacctgctg caagcgatag
     3781 agcatcggct gatgcatact gctggcccag aaggccagcc aggctttcat cgccgcactg
     3841 ctcacctggg tttcatcgaa gtttccacca acaatcgcct gtaatcgcag ctctgcactg
     3901 ccctgcggaa gtgcatgtaa tcgattcaaa accgcgtcac gcagctgact ggtgatatcg
     3961 cgcatggttg cttccagcag accatttttg tccctgaaat agtggctgat gatccccgta
     4021 gaaacgcctg cacggcgggc gatctgcgcg atcgttgcat cgtgcatgcc cacttcattt
     4081 attgcttcca gcgtggcgtc gatcagttgt ctgcgccgga tcgactgcat ccccaatttg
     4141 ggcattttcg ccactccatt catcagcgtt gtttatctat taaagcggtt attgattgga
     4201 cgttcaatat aaaatgtgtc ttaattgtta cgaatttgat tttaaatggt aacaataaca
     4261 gtggggatac tggatgacag acctttcaca cagcagggaa aaggacaaaa tcaatccggt
     4321 ggtgttttac acctccgccg gactgatttt gttgttttcc ctgacaacga tcctgtttcg
     4381 cgacttctcg gccctgtgga ttggccgcac gctggactgg gtttctaaaa ccttcggttg
     4441 gtactatctg ctggcggcaa cgctctatat tgtctttgtg gtctgtatcg cttgttcgcg
     4501 ttttggttcg gtgaagctcg ggccagaaca atccaaaccg gaattcagtc tgctgagttg
     4561 ggcggcgatg ctgtttgctg ccgggatcgg tatcgacctg atgttcttct ccgtagccga
     4621 accggtaacg cagtatatgc agccgccgga aggcgcggga cagacgattg aggccgcgcg
     4681 tcaggcgatg gtctggacgc tgtttcacta cggcttaacc ggctggtcga tgtatgcgct
     4741 gatgggcatg gcgctcggat actttagtta tcgttataat ttgccgctca ccatccgctc
     4801 ggcgctgtac ccgatcttcg gtaaacggat taacgggccg attggtcatt cagtggatat
     4861 tgcagcggtg atcggcacca tcttcggtat tgccactacg ctcggtatcg gtgtggtgca
     4921 gcttaactat ggcttgagcg tactgtttga tattcccgat tcgatggcgg cgaaagcggc
     4981 actgatcgcc ttgtcggtga tcatcgccac tatctcggtg acatccggtg ttgataaggg
     5041 cattcgcgtg ttatcggaac tcaacgttgc gctggcgctg ggattgatcc tgttcgtgtt
     5101 gtttatgggc gacacctcgt tcctgcttaa tgcactggtg ctgaatgttg gcgactatgt
     5161 gaatcgcttt atgggcatga cgctcaacag ttttgccttc gaccgtccgg ttgagtggat
     5221 gaataactgg acgctcttct tctgggcatg gtgggtggca tggtcgccgt ttgtcggctt
     5281 gttcctggcg cgtatctcgc gtgggcgtac cattcgccag ttcgtgctgg gcacgttgat
     5341 tattccgttt accttcacgc tgttatggct ctcggtattc ggcaatagcg cgctgtatga
     5401 aatcatccac ggcggcgcgg catttgccga ggaagcgatg gtccatccgg agcgcggctt
     5461 ctacagcctg ctggcgcaat atccggcgtt tacctttagc gcctccgtcg ccaccattac
     5521 tggcctgctg ttttatgtga cctcggcgga ctccggtgcg ctggtgctgg ggaatttcac
     5581 ctcgcagctt aaagatatca acagcgacgc ccccggctgg ctgcgcgtct tctggtcggt
     5641 ggcgattggc ctgctgacgc tcggcatgct gatgaccaac gggatatccg cgctgcaaaa
     5701 caccacggta atcatggggc tgccgttcag ctttgtgatt ttcttcgtga tggcggggtt
     5761 gtataaatct ctgaaggtag aagattaccg ccgtgaaagt gcgaaccgcg ataccgcgcc
     5821 gcgaccgctg gggcttcagg atcgcctgag ctggaaaaaa cgtctctcgc gcctgatgaa
     5881 ttatccgggc acgcgttaca ctaaacagat gatggagacg gtctgttacc cggcaatgga
     5941 agaagtggcg caggagctgc ggttgcgcgg cgcatacgtg gagctaaaaa gcctgccgcc
     6001 ggaagaggga caacagttgg ggcatctgga tttgttggtg catatgggcg aagaacaaaa
     6061 ctttgtctat cagatttggc cgcagcaata ttcggtgccg ggctttacct accgcgcacg
     6121 tagcggtaaa tcgacctact accggctgga aaccttcctg ttagaaggca gccagggcaa
     6181 cgacctgatg gactacagca aagagcaggt gatcaccgat attcttgacc agtacgagcg
     6241 gcaccttaac tttattcatc tccatcgtga agcgccgggc catagcgtga tgttcccgga
     6301 cgcgtgattg ttattgcata aaaccgcgcc atgtatgcat atggcgcggt ttctcttacc
     6361 tcttgatacg ttatatctgt acagttaagc ccttagtata gattgatgat tgccaggcaa
     6421 cagataataa aaaagaaaag aactattgaa gcccaaaacc tacgtttgga ctgttgtgaa
     6481 tgttcaataa gcttagtctt aattaatgta aatattggtg ctaatttgaa atgacgcttt
     6541 attcatgggg aacgcgtagc gcaaatcaac aaaaatcaat gtaaccattt gttgtttaag
     6601 gtttaattgt ctgtgtctga tattttattg attgttttaa atattgtttt tattgtcatt
     6661 gttataatgt tggtcatcta ttgctgaatg gattcagtct taagaggcag gttgttaagg
     6721 gataggcata gagtaatgac gtgtatgcat aaccaacatc tttactcatt atgtcattga
     6781 atgttgacgc tatgtgttta tgaggttgag gtattttcag ttgatctgga ttgttaaatt
     6841 catataatgc gcctttactc atgaatgggc gtcaatacgt agtgggaaat tataaatatc
     6901 gaaatagtcc aactacttct ttattaccaa aaatgagtat ctgaatttta atattgcatt
     6961 cttgcgtgat tatctcctga gttttgactt gtgattacct ttttaaggta tttagcgtaa
     7021 ctgtttctga gcgagcatca gaggtaaaga taatcttctt gatagtgatg tgggatgtta
     7081 tacgtatggc atcgctaatg tttatggtta ccccttatgt gtgctcagga atccacaggt
     7141 aatcactcat actgaacagc gataaaagat aaaaggtgtg ttcatgaatt catgtgattt
     7201 tcgtgttttt ctgcaagagt tcggtacaac ggttcatttg tcattgcctg gtagcgtatc
     7261 cgagaaagaa cgactgctac tcaagctgct gatgcaggga atgtctgtaa cagaaatatc
     7321 acagtacaga aatcgtagtg caaagaccat ttcacatcaa aagaaacagc tctttgagaa
     7381 actggggatt cagagcgata ttactttctg gcgggatatt ttctttcagt acaatccgga
     7441 gatcatatcc gccacgggta ataatagtca caaatatatt aatgataatc actatcacca
     7501 tatcgtcacg cctgaagcca tcagtctggc gttggaaaac catgaattta aaccgtggat
     7561 ccaaccggtt ttctgcgcgc agactggggt actgacgggc tgtgaggtgc ttgtccgctg
     7621 ggaacatcca caaacgggaa ttatcccacc ggatcagttt attcctctgg cggagtcatc
     7681 cggtcttatt gtcataatga cccgccagtt gatgaaacag actgcggata ttctgatgcc
     7741 ggtaaaacat ttgctgccgg acaatttcca tattggcatc aacgtctcgg cgggttgttt
     7801 tttggctgcg ggatttgaaa aagagtgtct gaacctggtt aagaaattag gtaacgataa
     7861 aatcaaactg gttctcgagc taacggaacg taaccctatt ccggtaacgc cagaagccag
     7921 agcgatattt gacagccttc atcagcacaa cattaccttt gcgctggatg actttggtac
     7981 gggttatgcg acctatcgtt acttgcaggc gttcccggtc gattttatta agatcgataa
     8041 gtcatttgtg caaatggcga gcgtggacga aatatccggt catattgtgg acaatattgt
     8101 cgaactggcg cgtaagcctg gtctgagtat cgtggcggaa ggggtagaaa cccaggagca
     8161 ggcggattta atgatcggca aaggcgttca ctttttgcag ggctatttgt actctccgcc
     8221 agtaccgggt aataaattta tctctgaatg ggtaatgaaa gcaggtggtt gatgtaaacc
     8281 gctattcaca gcgcatcggg aggttggcag cgattaattc tcccgatgca gtaaatcctg
     8341 atagatcccc gttaatatgc catttggcga aaattgtttt ttaatccact gcgtcacctg
     8401 acccgtagca gaatgctgag ttgcgagtaa catgcgtgaa tcctggcgcg gattatggat
     8461 ttgtcgggta accagcaggg attgcgccat cgcctcacgg accatgtaat ccggcaaaaa
     8521 accaattcct tcacctaaaa tctggcactg acatttggtg ttgaaatctg gcaccagaat
     8581 cgactcctgc ccatgcagca accagcccac ttttttatta atcgtatgcg cggtgtcctc
     8641 caccatgata ttggggtaaa gacgcagctg gctttcggcg atcggctccg gcacaaaggc
     8701 taatgggtga tccggggcga tagcaaaagc ccagcgaatt gcgccaatct ccgtgtaatc
     8761 aatgccgccg ccgtccagca gcgtatcggg tgcgccgata gcaatattcg cctgattatt
     8821 aataatcgca tcccagacgc cgttatacac ttcggtcgtc acggtaatct ggcaggttgg
     8881 aaactgcttt ttcagtacct gtaataagcg tgcggtgtgt ttgggcgtat aaagcagttg
     8941 gttaatacaa atgcgcaccc gtgcttctat accctgcgaa atagtatcga tgctgcgttt
     9001 gatggcgtga aaatcattca gcaggtcggt agctttgcgg aaaaaataac gcccggactc
     9061 cgttaactca atgcttcggg tactgcgagt gaacagcacc acatccagcc ctgtttccat
     9121 tcgcttgatg gtgtagctaa tggcggaagt ggttaaaccc aactcttctg cggctttact
     9181 gaaactgcca aaacgcgcag cggtagtaaa tgccaacaga ttctcttcgg taaaaattga
     9241 gttcattaaa ccattcccgc cattatcgaa tatgaatttc tccagctatg actgtagagg
     9301 tatcggttaa agatagataa tcatttttga ataactttta atacccgtcg cgtttagtcg
     9361 tcgcttcgct taatttttgt ccgccatatt ctgaaattat ccccacgcag ttacccgcca
     9421 gaaaacccag taatgtcacc gtccagttca tcccgctggc aaaaaatagc gtcatgccga
     9481 gaaagcctcc gggaataaaa gacagtaaac cgaaacgtcc ttgccagacc accaccgccg
     9541 agaagggtac agtcataatc acgctcgccc agaacccgga aatcccactt acagaggcca
     9601 gccagccggc agccagcgcg gcgaaaaagg cccacaccat cccgaaataa ttaaccgcca
     9661 gacttctgat aaaccccgat ttaccgccgc ctgcggcata aaaactgcaa aaggcgacga
     9721 agccaatggt tcctaataat tcccatccct gagagagccc gacatgtgaa gacactaact
     9781 gccaaagccc tgcacaaata ccgacggtga cacccgttgc cgtgagtccg ttcatcttgt
     9841 tcccctctgg ttagacatgg acgaaagcta ctcttacatg tgcagactat agagaaataa
     9901 aacctgattg agtgaaaggg tatcgggtca aagaaacaaa tattgcacta ccgcacactg
     9961 cgaaaagatt gttgaacatc attcaacaaa aaggcgaaat acggcgaatt aaatttaagc
    10021 gggttctttt atcagattat cgttgctatt ctcaattcat cgaaaacagc cacaaaaacc
    10081 aggggaatgt gaatgactat aaaaaatcta cctgccgact atttattggc tgcgcaacag
    10141 ggtgatatcg ataaagtaaa aacctgcctt gcgctgggtg tcgatattaa tacctgcgat
    10201 cgtcagggga aaacggcaat tacgctggca agtttatatc agcaatatgc ttgcgttcag
    10261 gcattaattg atgccggagc ggatattaat aagcaagatc atacctgttt aaatcctttt
    10321 ttaattagct gtctgaacga tgatttaacg ctactacgaa ttattttacc ggctaaaccc
    10381 gatcttaatt gcgtcacccg ttttggcggt gtcggcctga cgcctgcctg tgaaaaaggc
    10441 catttaaata ttgtaaaaga gcttttggcg catacggaga ttaacgttaa ccagaccaac
    10501 catgttggct ggacgccgct actggaagcg attgtgctta atgatggcgg gattaaacag
    10561 caggcgattg tccagttatt gctggaacac ggtgccagcc cgcacatgac ggataaatat
    10621 ggcaaaacgc cgctggaact ggcgcgggaa cgaggctttg aagagattgc gcagttactg
    10681 attgccgcag gtgcataaac cgggaggctt gctatcaaca caccagaaag acggtgtgtg
    10741 tgggcgctaa ctgcggatgc tgattttctg gcgcagcggg ggcaaggaca ggttgaacag
    10801 gtctttgcca gagcggtaaa tatcgcactt ccggcccgcc agcagttgct gacgctgctt
    10861 tgtgaagagt acgacaatgc gccaaacagt tgtcggttgg cactcactca ctttgatggt
    10921 ctgttccggc atggtgataa ggttcagttt gacgatcaag gcattacggt tggtcaacat
    10981 cttcatatag agatgagtcg ttgtcggcgt tggctgtccc caaccttgca aatggccgct
    11041 gtgaattttc accttatcgc ctggcaacag tggcatgaca ttattcatca gcacctgggg
    11101 gaaaatgaaa ccctgtttaa ttattgcggc gataatccgt tttatcaggc gttaaataaa
    11161 gaattacata ttaaacgacg ggcagttatt caggccgtaa acgataaaca aaatatcgcc
    11221 tcagcagtcg ccagtatgat gggcttaggg attggcctta cgccatcagc cgacgattat
    11281 ttaacaggtc tggcgcttat tttatttatt tccggacatc cggcggaaaa atacaaagag
    11341 gaattttatc tcggtctgca atgcggcaga aataatacca cgttattaag tgccataacg
    11401 ctggaagccg cattacaaca acgctgccgg gaaaatattc atcgttttat tcataacatt
    11461 atttatgaca tccctgggaa cgcaactcag gcaatagaaa aaattaaaca tattggctcc
    11521 agttccggct gcgacatgct gtatggcatg gccgatggtt gtgcgctgag ccaaacctac
    11581 ggagggaatt atgtcagtta aaatagtcat taaaccgaat acctattttg attctgtctc
    11641 gctgatgtct atctccacgc gtgcaaataa actcgacggc gtcgagcagg catttgtggc
    11701 gatggcgacc gaaatgaaca aaggcgtgct gaggaattta ggactgctga cgccggaact
    11761 ggagcaggcg aaaaacggtg acctgatgat tgtcatcaat ggtaaatcgg gtgcggacaa
    11821 cgagcagttg ctggtggaga ttgaagaact gttcaacagc aaagcgcaaa gtggctcgca
    11881 cgaggcgcgt tacgccacta ttgccagcgc caaaaagcat atcccggaaa gtaacctggc
    11941 ggtgatttcg gtcaacggtc tgtttgccgc tcgcgaagcg cgtcaggcgc tgcaaaacga
    12001 tcttaacgtg atgctgtttt ccgataacgt ctcagttgaa gatgaactgg cgctcaaaca
    12061 gttggcccac gaaaaagggc tgctgatgat ggggccagac tgcggtacgg cgattatcaa
    12121 cggcgcggcg ctctgctttg gtaacgccgt gcgtcgcggc aacatcggca ttgttggcgc
    12181 atccggcacc ggtagccagg aactgagcgt ccgcattcat gaatttggcg gcggcgtttc
    12241 gcaactgatc ggcaccggcg ggcgggacct gagcgagaaa atcggcggcc tgatgatgct
    12301 cgacgctatc gggatgctgg aaaacgatcc gcaaactgaa atcattgtgc ttatctccaa
    12361 accgccagcg cctgcggtgg cccgcaaagt gctggaacgc gcacgcgcct gccgtaagcc
    12421 agtggtagtc tgcttcctcg gtcgtggcga aacgccagtg gatgagcagg ggctacagtt
    12481 tgctcgcggc accaaagaag cggcactaaa agcggtgatg ctctccggta tgaaacaaga
    12541 acatctcgac ttgcataccc tgaaccagcc gttgattgcg gatgtgcgtg cgcgtatgca
    12601 accgcagcag aaatacattc gtggcctgtt ctgcggcggc acgctgtgtg acgaaaccat
    12661 gtttgcggtg atggaaaaac atggcgatgt ctacagcaac attcagcccg atcctgaatt
    12721 ccgcctgcaa gatatcaacc gcagcgtcaa acataccttc ctcgactttg gcgatgacga
    12781 ttttaccaac ggcaagccgc atccaatgat tgacccgacc aaccgcatca gccggttgat
    12841 cgaagaagcg cgcgatccag aagtggcggt gatcgtgatg gattttgtgc tcgggtttgg
    12901 atcgcatgaa gatccggtcg gttccaccat cgaggcgatc aaagaggcga aagcgatcgc
    12961 cgctgccgaa gggcgcgagt tgatcattct cgcctatgtg ctgggtaccg atctcgatac
    13021 gccatcgtta gaacaacaaa gccagatgtt gcttgatgcc ggagtgattc tggccagcag
    13081 cagcaccaat accggattgc tggcgcgtga atttatctgc aaaggggagg aagcctgatg
    13141 agcccgtcac tgtttagcca accgttgaac gttattaacg tcggtatcgc catgtttagc
    13201 gatgatctga aaaagcagca tgtagaagtg acccaactcg actggacgcc gccggggcag
    13261 ggcaatatgc aggtggtgca ggcgctggac aacattgccg attccccgct ggcggacaaa
    13321 atcgccgccg ctaaccagca ggcgctggag cgcattatcc agtcgcatcc ggtgctgatt
    13381 ggttttgatc aggcgattaa cgtggtgccg ggtatgacgc cgaaaaccat tcttcacgcc
    13441 gggccgccga tcacctggga aaaaatgtgt ggcgcgatga aaggcgcggt caccggagcg
    13501 ctggtgttcg aagggctggc gaaagatctc gatgaggcgg ctgaactggc tgcctccggg
    13561 gagatcacct tctcgccgtg ccacgagcac gactgcgtgg gttcgatggc gggtgttacc
    13621 tcggcctcga tgtttatgca catcgtaaaa aacaaaacct acggcaacat cgcttacacc
    13681 aatatgagcg agcagatggc gaagattttg cgtatgggcg ctaacgacca gagcgtgatt
    13741 gaccgcctca actggatgcg cgatgtgcag gggcccatac tgcgcgacgc gatgaaaatt
    13801 atcggcgaaa tcgatctgcg cttaatgctg gcgcaagcgc tgcatatggg cgatgagtgc
    13861 cataaccgca ataacgccgg gacgacgctg ctgattcagg cgctgacgcc agggattatt
    13921 caggcgggct attccgtcga acaacagcgg gaagtgtttg agtttgtcgc cagcagcgac
    13981 tacttctccg gcccgacgtg gatggcgatg tgtaaagcgg cgatggatgc ggcgcacggc
    14041 attgaataca gcactgtggt caccaccatg gcgcgtaacg gcgtcgagtt cggcctgcgg
    14101 gtcagcggcc tgccggggca atggtttacc ggcccggcgc agcaggtgat cggcccgatg
    14161 tttgccggtt ataagccgga agattcgggg ctggatatcg gcgacagcgc catcactgaa
    14221 acctacggca ttggcggatt tgccatggcg accgcgcctg caatcgttgc gctggtgggc
    14281 ggcacggtgg aagaggctat cgatttctcc cgccagatgc gtgaaatcac tctcggtgaa
    14341 aaccccaacg tcaccattcc gctgctcggt tttatggggg tgccgacggc aatcgacatc
    14401 acccgcgtgg gcagtagcgg cattctgccg gtgatcaaca ccgccatcgc ccataaagat
    14461 gcgggcgtcg gcatgattgg cgcgggcatt gtgcatccac cattcgcctg cttcgagaaa
    14521 gccattcttg gctggtgcga acgttacggc gtctgagtta catgtttatg ccggatgcga
    14581 catgaatgtc ttatccggcc tacgaatggc gcggaaatct gtaggcctga taagcgtggc
    14641 gcttcaggca ttggccacca cagccggatg cgacgtgaac gccttatccg gcctacgaat
    14701 ggcacggaaa tctgtaggcc tgataagcgt agcgcatcag gcattggtca ccacagccgg
    14761 atgcggcgtg aacgccttat ccggcctacg agtggcgcaa gaatctgtag gcctgataag
    14821 cgtagcgcat caggcagtct ggcgttggtc ataaccccat caccctctgt agcggacata
    14881 aaaatatgaa agaacttgtg gtcgttgcca ttggtggcaa cagcattatc aaagataacg
    14941 ccagccagtc gattgagcat caggcggagg cggtgaaagc cgtcgccgat acggtgctgg
    15001 aaatgctggc ttccgattac gacattgtgc tgacccacgg taacgggccg caggtcgggc
    15061 tggatttacg ccgcgcggag attgctcacg agcgcgaagg gctgccctta acgccgctgg
    15121 caaactgtgt ggcggatacg caaggtggca ttggctatct gatccagcag gcgctgaaca
    15181 accggctggc ccgccacggc gagaaaaaag ccgtcaccgt ggtgactcag gtggaagtgg
    15241 ataaaaacga tccgggattt gcccatccca ccaagcctat cggcgtattc ttcagtgaaa
    15301 gccagcgtga cgaattacaa aaggcaaacc ctgactggcg ttttgttgaa gatgccggac
    15361 ggggctatcg ccgcgtggtc gcctcgccgg aaccgcaacg tattgtagaa gcacctgcca
    15421 ttaaggccct gatccaacaa ggctttgtgg tgattggcgc gggcggcggc ggaattcctg
    15481 tggtgtgcag tgaagcggga gattaccaaa gcgtggacgc ggttatcgac aaagatctct
    15541 ctaccgcgct attggcccgt gaaattcacg ccgacattct ggtaattacc accggcgtcg
    15601 aaaaagtgtg tattcacttt ggcaaaccgc agcagcaggc gctggatcgg gtggatattg
    15661 ccaccatgac ccgctatatg caggaagggc atttcccacc cggcagcatg ttgccaaaaa
    15721 tcatcgccag cctgacgttc ctggagcagg gcggtaaaga agtgattatc accacgccgg
    15781 aatgcctgcc tgcggcgctg cgtggtgaaa cgggcaccca tattattaaa acgtaaggac
    15841 gtaagatgaa agaaaacaat agccgccgtg agtttctgag ccagagcggt aagatggtca
    15901 ccgccgccgc gctgtttggt acctctgtgc cgctcgccca tgcggcagtc tctggcacaa
    15961 tccattgcga cgcgaataac accatgaaaa tcactgaccc gcactactat ctcgacaacg
    16021 tgttgctgga aaccggtttt gactacgaaa atggcgtggc ggtgcagacc cgcacggcgc
    16081 gccagactgt ggaaattcag aacggcaaaa ttgttgccct gcgcgagaac aatcagcatc
    16141 cggacgccac gctgccgcac tatgacgctg gcggtaagct gatgctgccc accacccgcg
    16201 acatgcatat tcatctcgac aaaaccttct atggcgggcc gtggcgctcg ctcaatcgtc
    16261 cggcaggcac caccatccag gacatgatca aactcgagca gaaaatgctg ccggagttgc
    16321 aaccgtacac tcaggaaagg gcggaaaaac tgattgattt attgcagtcg aaaggcacta
    16381 ccattgcccg cagccactgc aatatcgaac cggtttccgg cctgaaaaat ctgcaaaatt
    16441 tgcaggcggt tctggcgcga cgtcaggcgg gcttcgagtg tgaaattgtc gccttcccgc
    16501 agcacggttt gctgctgtcg aaatcagaac ccttaatgcg cgaagcgatg caggcggggg
    16561 cgcattacgt cggcgggctg gacccgacca gtgttgatgg cgcgatggaa aaatccctcg
    16621 acaccatgtt ccagattgcg ctggactacg acaaaggcgt cgatattcac ctgcacgaaa
    16681 ccactccggc aggcgtggca gccatcaatt atatggttga aacggtagag aaaacgccac
    16741 agctgaaggg caagctgacc atcagccacg ccttcgcgtt ggcaacgctc aacgaacaac
    16801 aggtagatga actggcgaac cggatggcgg cgcagcaaat ttctatcgcc tcgacggtgc
    16861 agatcggcac gctacatatg ccgcttaaac aactgcacga caaaggcgta aaagtcatga
    16921 ccggaactga cagcgttatt gaccactggt cgccttacgg cctgggcgac atgctggaaa
    16981 aagccaatct ctacgcgcag ctctatattc gtcctaacga acagaacctc tcccgttcgc
    17041 tgtttttagc tactggcgat atattgccgc tcaacgaaaa aggcgagcgt gtgtggccta
    17101 aagctcagga tgacgccagc tttgtgctgg tggacgcctc ctgttccgcc gaggcggtgg
    17161 cgcgtatctc gccgagaacc gcaacgttcc ataaagggca actggtgtgg gggaatgtgg
    17221 caggttgatg cgcatttgat atgtctgcaa cggtgaacag taagagattt aagccccagg
    17281 gagtggggcg ggaatggttt gttaatgaat gatcctgaac tttataatct cccggcataa
    17341 tttccagggg attatttgag agaaatacac aaaagtgagt tttaacaaag gtatttatca
    17401 agccataaat gtgacctgct tcggataaaa aagattaaac ataggaaaac caatgatact
    17461 cttgctgatg gttaccaaca gtaattaaga tgtgattttt tccgtaatcg aaaaaatatt
    17521 gccatgacca taaaagaaaa attctcacaa aaatatcccc atgcttcctt ttgtactttt
    17581 ggcgattcag cagcgttggc cgatcacctc gcaacgttga ttgcgactgg tgttaaaaca
    17641 gcttcctgtg gttcgctggc gggatgtatt gaagacaacg catttccgct gattggtgag
    17701 tataagattg tagaaaatag ccgtggtgag cctgtctgtg ttatcagagt gattggcctg
    17761 catttactgc gcttttctga tgtcaccgca gagcttgccc gtaaggaagg tgaaggtgac
    17821 ctcagtcttg aatattggcg taatgaacac cgtcgttttt tccaggcaga aggatgctat
    17881 tcaccagaaa tggacgttat ctttgaagaa tatgccctta ttgacgttgt gtagataact
    17941 atttttctac gcgaaataag ctcacaaaca taaagtatcg ctaattttgt ttattttgct
    18001 gttttttacg tgatgattgt cacacttatt gttgctgccc gttttgtcca tatttataac
    18061 caaattttta atagctgcct gaaatagggc tgtttattct gcgtaaaaat attcgaaaat
    18121 tcttattctc tccagcgcgc taatttgggc gaacacttcc tgactaccct gcaatgaggc
    18181 tgaaatgatg aataaacgtt ttgttatcaa tatggtgtct tcgctgttat taggcgctgc
    18241 gctaatttct gcgccgctcc aggcagcgga aaaagtcgtg gtgaatatct ctaaagtcga
    18301 tggtatgccg tggtttaatc gcatgggcga aggtgtggtt gaggcgggta aagcctttgg
    18361 tgttaatgcg tcacaggtgg ggccttcaag tactgatgct ccgcaacagg ttaaaattat
    18421 tgaggattta atcgcccgta aggttaatgc aattactatc gtgccaaacg atgccaacgt
    18481 gctggaaccg gtattcaaaa aagcgcgaga tgccggaatt gtggttctga ccaacgaatc
    18541 tcccggtcag ccaagtgcta actgggatat cgaaatcatt gataacgaaa aatttgccgc
    18601 tgaatatgtt gagcatatgg cgaaacgcat ggggggcaag ggcggctatg tgatttacgt
    18661 gggtagtttg accgtgccgc agcataactt atgggctgac ttgctggtga aataccagaa
    18721 agagcattac ccggatatgc atgaagttac ccgccgcatg ccggttgccg agagtgtgga
    18781 tgactcacgt cgtaccacac tggacctgat gaaaacctat cctgacttga aagcggttgt
    18841 ctcctttggt tcaaacggtc cgattggcgc gggacgtgca gtgaaagaga aacgggcgaa
    18901 aaataaagtc gcagtctatg gcatgatgat cccttcacag gcggcatcac tgattaaaag
    18961 tggtgatatc accgaaggga ttacttacga tccggcctct gcaggttacg cactcgcggc
    19021 cgtcgccagc acgttgctga agggtgaaga gatcaaacct ggattagaaa tgcagaatct
    19081 cggtaaggcc gacgtagata tggataagcg aattatccgt ttccataagg tattactggt
    19141 caacaaagac aatatcgact cactttatta atccatttct tcggggcgaa agccccgatt
    19201 cttattgcca ggggatgcta tggaaacctt cctttccctt cgtcatatca acaaaacgtt
    19261 tcatgccacg cgagcgctgc gcgatgtttc tctcgacttt atgtcgggtg aggtgcactg
    19321 ccttgccggg caaaacggct gtggtaaatc gacgttgatt aaaattatgt ccggtgttta
    19381 tcgcccggat gagggggcgg aaattacgct tggtgggaaa aactggtcaa agctgacacc
    19441 cgccgcttcg gtggcgcagg ggattcaggt gatttatcag gacctctctt tatttcctaa
    19501 cctgagtgtc tgggagaata tcgccgtgaa tcactatcac cacggtctgt ttgttaaccg
    19561 ccgtcgtctg cgtgaggtgg cgcaggcggc aatgaccagt attaacgtca cattgccact
    19621 ggatacgctc gtttctgagc tttcaattgc ccgctgtcag ctggtggcaa tttgccgtgc
    19681 actggcgcag gacgcacggt tgatcgtgat ggatgaacca accgcttcgc tgacccatca
    19741 ggaagtgcaa gggttattac aggtggtgca tcaattgcgg gaacgcggga tctgcgtggt
    19801 cttcgtcagt catcgtctgg aagaagtgat ggaagtttcc gaccgtattt cagtgctgaa
    19861 agatggtgag ctggtcggga cctttccggc cgcagagatg accacaaaac aactcagttt
    19921 tctgatgacc ggccaggagt tcgaatatca ggtacgagag ttgtggcagg gaaaatccag
    19981 caccccggtg ctggaggtgc gtaatttaag tcgtcatggg gaatatcaaa atattaactt
    20041 gcgggtggaa gcgggcgaag tggtgtcgat tgttggcctg cttggtgcgg ggcgcacgga
    20101 attatgtctg agtctgtttg gtatgacccg accggatgct ggtgagatcc tcatcaacgg
    20161 tcagccggtt acactgcata gtaaccagga cgccatccgc cacggtattg gttatgtttc
    20221 ggaagatcgc atgtcgcgcg gtttggtgat ggcgcaatct attgaagaca acatcattag
    20281 cactgttttt cacaaggtaa aagatcgctt cggctttctg agtgaagcta aagtgcgtga
    20341 cctggtggac aggctaatca aggcattgac catcaaagtc tctgacccac atctcccggt
    20401 gaatacgctt tccgggggta acgcgcagcg agtatcgatc gccaaatggc tggcgattgg
    20461 gccacgactg ttgattctcg atagcccgac tgtcggtgtt gatatcgcca acaaagcagg
    20521 aatatacggc attatcagcg atctggcggc tcatggcatt gcggtattga tgatctgcga
    20581 tgaaattgaa gaagcgtggt atcaaagcca ccggattttg gtaatgcaaa aaggtcagat
    20641 cacccatagc ttcctgcctg acagcagttc tcaggccagg attgcggagg tggtaaatgg
    20701 ctgaactgaa aaaacgccat gaattctggc tggcattact gattgtggtg ctgtttgttg
    20761 gccttgcctg gcgcagcgac gagtttctga cattcggtaa tttgtacgat ctcgccaata
    20821 actatgccat gttgactatt ctcgcctgtg gtttgtttgt ggtgttaatt tccggtggga
    20881 ttgatatttc gtttccggca atgaccatca ttgcgcaata cggcatggtg ctgttgctgc
    20941 aaaaaatggg tggcaacttt gctgtcgcgt ttgccctggc gggcggcatc ggcattttac
    21001 ttggcttaat aaacgcctta ctggttaatc gcctacgggt gccgtctatc atcatcacta
    21061 tctcgacgct gaatattttt tatggcctgc tgttatggtt gagtaaaggt gtgtggctgt
    21121 acgactttcc gccgtggttt gagcaggggg taatgttgtt caagtacacc gatgctgatg
    21181 gctatgacta tggccttggt ctgccgctga tcgccatgat tacggtggtg ctgctgacag
    21241 cgtttatcat gaatttcacc agtgtagggc gcaaaattta tgcccttggc gggaaccgcg
    21301 aatcagccag tcgcatcggc tttagtgtgc tgaaactgca acttttcgtc tatggctata
    21361 tgggattgat gtctggtgct gcgggtgtag tgcagtcgtg gacggtgatg actgtcgccc
    21421 ccgattctct tctgggttat gagctgacag tactggctgc ggtggtgctt ggtggcacta
    21481 gtttgctcgg cgggcgcggc acgttaaccg gtactttgct cggcgtggtg ttgttggcag
    21541 taatgcaaaa cgggctaaat ttattgggag tctcgtctta ctggcaaaca ttgatcacag
    21601 gcatcatcat cgttgccagc attagcgcca cggcgtggag tcagcatcag aaccggagtc
    21661 tgctatgaaa aaatcgtggc gtaataacgt tgagttttac ttgatcggcc tgctggtgct
    21721 aactgttgcc gcgttcagca ttactatgcc ggagattttc tggtcgataa gtaatttcca
    21781 gtccgtcgct tcgcaaatgc ccgtgctggg cattctggcg ttggctatgg cggtgaccat
    21841 gctttgcggt ggcattaact tgtcaattat agccactgct aacgcctgtt cactggtgat
    21901 ggcgtgggtg gcgactcagt atccaccggg tattgctacg gttgtcgcta cgctgctggc
    21961 gggcgctggc gcggcggtga taatcggttt atgtaacggc gtattgattg ctggtattcg
    22021 agtgtcgcca attcttgcaa cgcttgggat gatgacgttg ctcaaagggg taaatattct
    22081 ggtgacaggc ggtagcgcca ttgccaatta tccctcatgg gtgctgtggt tgaatcatgc
    22141 acagtggttc ggtattccgc taccgatgtg gttgtttacc gcagtggcgc tggggctgtg
    22201 gatattgctg gagaaaacgc cgctcggtaa atccatttat ctgattggtt ctaacgaacg
    22261 agcaacactt tactccggta ttaacactcg tcgggtgctg atatgggtgt atgtcatttc
    22321 cgccttgctt tgtgcggtag cggcattttt gatgatgtcg aaactcaact cggctaaggc
    22381 ctcttatggc gaatcttact tgctagtgtc aatcctcgcg gcagtactcg gcggggttaa
    22441 tccggacggc ggcagcgggc ggataattgg tatggtgctg gcgctgtttc ttctgcaaat
    22501 tattgaaagc ggcttcaata ttctcggtat aagtccctac ctgacgatgg ccttatgggg
    22561 aacgttgctg ctctgtttca ttcaggctcg cggcatgttg gggctggatc gggtggttta
    22621 aatattggct atattcaatg gacgcgtttt gccgcgatga catatcaggc atcgccaaat
    22681 acacatagct aatcaggagt aaacacaatg aagatcaaag ctgttggtgc atattcagct
    22741 aaacaaccac ttgaaccgat ggatatcacc cggcgtgaac cgggaccgca tgatgtcaaa
    22801 attgaaatcg cttactgtgg cgtctgccat tccgatatcc accaggtccg ttccgagtgg
    22861 gcggggacgg tttatccctg cgtgccgggc catgaaattg taggacgcgt ggtagctgtt
    22921 ggtgatcagg tagaaaaata tgcgccgggc gatctggttg gtgtcggctg cattgtcgac
    22981 agttgtaaac attgcgaaga gtgtgaagac gggctggaaa actactgtga tcacatgacc
    23041 ggcacctata actcgccgac gccggacgaa ccgggccata ctctgggcgg ctactcacaa
    23101 cagatcgtcg ttcatgagcg atatgttctg cgtattcgtc acccgcaaga gcacctggcg
    23161 gcggtggctc ctttgttgtg tgcagggatc accacatact cgccgctacg tcactggcag
    23221 gccgggccgg gtaaaaaagt gggcgtggtc ggcatcggcg gtctgggaca tatggggatc
    23281 aagctggccc acgcgatggg ggcgcatgtg gtggcattta ccacttctga gttaaaacgc
    23341 gaagcggcaa aagccctggg ggccgatgaa gttgttaact cacgcaatgc cgatgagatg
    23401 gcggctcatc tgaagagttt cgatttcatt ttgaatacag tagctgcgcc acataatctc
    23461 gacgatttta ccaccttgct gaagcgcgat ggcaccatga cgttggttgg tgcgcctgcg
    23521 acaccgcata aatcgccgga agttttcaac ctgatcatga aacgccgcgc gatagccggc
    23581 tctatgattg gcggcattcc agaaacacag gagatgctcg atttttgcgc cgaacatggc
    23641 atcgtggctg atatagagat gattcgggcc gatcaaatta atgaagccta tgagcgaatg
    23701 ctgcgtggtg atgtgaaata tcgttttgtt atcgataatc gcacactaac agactgaaaa
    23761 aattaataaa catcctgtgg tttaacatat taacttcgct ccacttaact ttttagttaa
    23821 ggagagcgaa ataatatcaa agtagtggta aacctatagc gtaaattcag attgttaaat
    23881 taactccact actttacacg ttagttatga gcgatttctg atagtgtctg gtttaatcag
    23941 agctttatta tctgcgacgt ttatttttat ttaatagagc cacctgcaag ttgttattcc
    24001 accagagagt gaatcgcaaa gcaggtaatc atttcatctg tatatctgta tgccaactgg
    24061 cattccatct cttttttcgc cgatattttg ttgtgatcgg cgacacttcg cttaaaaaag
    24121 catcagcagt ggtttcgcag ctatgcggtg tataaaaaat gatctcatgc agatgttttg
    24181 tgaatgtgtg ggttgacatt catatgaaaa aaatcataat tccatcatgt ttgtgtgggg
    24241 tcttttctgt atcttacgca tcgcactcaa gcctgacaga aaatatgctg taaggctcat
    24301 atcaaaaacc gccattagct catcaggaag agcagacgac aaccaggatt gttgtatggt
    24361 acggggttcg aggcctcgat ggcagtccag cttttcaaaa ctcttctgaa tcagatcccc
    24421 ttattatcat tcctgcaaag tggaacattg ccattgtttg gctattcagg acgggtcaga
    24481 ccaatgaaaa aagcccacac aggggagagt gggctgaaat gggaagctaa agactcaagt
    24541 aaacttatcg gaaataagga ccacgcatta cgggggctat catcgccgat ggtcgggatt
    24601 cggcaaatca ggttgattac gtagagagta aattatctgc tcaccgctgc gtcacccctt
    24661 cgtaaatcag gcgtagcgcg aatacaccaa taattgcacc aataacccga ctggcaacgc
    24721 gttgcatacg cccataagca cgacgcactg cgggcaaaga aaacgcctga ctaagaaaaa
    24781 cgcgccagat aactgatgcg agcacaatcc ccgcccaggc cattaaacgt gcccatgttg
    24841 gtgtttcggc atttaatgtt actgagaaaa tactaataaa aaataaaacg gtttgcgggt
    24901 tagagagatc ggtaattaat ccacggcgga aaaagatata ccagggggca ctaatcggtt
    24961 gttgtagtgt gctcatttgc ggtgttgact ggcggcgcat gctgcaccac gcaaaccata
    25021 agagataagc gccgccgacg attctgataa gcgaaaaaat ctcctcacac tgcgtaatta
    25081 gtgttgcaag accaaacaaa cccaacccgg aataaaatgc atcgcccaac gccacgccca
    25141 gtccggtcag cacccctgcg cgtcgaccgg aagccaggct ggtttgtact accacaaaga
    25201 gattggctcc cggattaaaa aaagtaatca cgaacagtcc tacggtcaga taaacggcgt
    25261 gcaaaggatc catagtgatt tcaaccataa ataagtgaag taacttcatc atatttctac
    25321 caaaaataat cgggtgcgag agagatcaca aagtgtctta tttccggtta ctggcgttta
    25381 tgccctgact gaactaatta ttaatcaacc caataatgtg ggtgggtgat agtgtgataa
    25441 caactctgga gccgtaatat gaaaataatc tctaaaatgt tagtcggtgc gttagcgttt
    25501 gccgttacca atgtctatgc cgcagaatta atgaccaaag cggaatttga aaaggttgca
    25561 tcgcaatatg aaaaaatagg tgatatttca accagtaatg aaatgtcgac cgcagatgcg
    25621 aaagaagatt tgatcaaaaa agccgatgaa aaaggggctg atgtgttggt actgacctct
    25681 ggtcaaactg acaataagat ccacggcacg gcaaatattt ataagaagaa gtaattctga
    25741 atcctatgta aacatccccg atgcgtaagt ttatcggtga tcatcatcta ttgaaattta
    25801 tgccggataa agcgttcgcg ctgcattcgg cagttcagct tttcagccgc cgccagaacg
    25861 tcgtccggct gatacctaaa taattcgccg ctgctgtttt atcgccatta aatttctcca
    25921 gtgcctgttg tgctgtcagc aagcctggaa tgggagtctt cgccgactcg cgtgccagtt
    25981 ccggcagtag cagctgcaaa aattgcggcg ttaaatccgg cgtcgattcc acacttaaaa
    26041 acagcgccag tcgttccatc atattgcgca gttcacgaat attgcccggc cagtcgtagt
    26101 gcaccagcat ggtttcgctt tcctgtaatc cctggcgtaa tgcggcagca aacggggcgg
    26161 agagcgccgc cagagacact ttcaaaaaac tttccgccag cggcagaata tccgccaccc
    26221 gctcgcgcag tggtggcaat tgcagacgca aaatactcag ccgataaaac agatcacggc
    26281 gaaactgccc ttgccgcata tcttcttcca gattgcagtg agtggcgcta atgactcgca
    26341 catccaccgg aacaggctga tgcccgccga cgcgggtgac ctctttttct tccagcaccc
    26401 gcagcagccg agtctgcaac ggcagcggca tttcgcctat ctcgtcaaga aacagcgtgc
    26461 caccgtgggc gatttcgaac agcccggcgc gacctccgcg tcgcgagccg gtaaacgccc
    26521 cttcctcatg gccaaacagc tccgcttcca gcagcgattc ggcaatcgct ccgcagttga
    26581 cggcaacaaa cggatgcgac tttttgccct gtcgcgcatc gtggcgggca aaatattccc
    26641 gatgaatcgc ctgggccgcc agctctttgc ccgtccccgt ttccccctca atcaacaccg
    26701 ccgcactgga gcgggcatac agcaaaatag tctgccgcac ctgttccatc tgtggtgatt
    26761 gaccgagcat atcgcccagc acgtaacgag tacgcagggc gttgcgggtg gcatcgtgag
    26821 tgttatggcg taacgacatg cgcgtcatat ccagcgcatc gctgaatgcc tggcgcacgg
    26881 tggcggcgga atagataaaa attccggtca ttccggcttc ttctgccaga tcggtaatca
    26941 gccccgcgcc gaccaccgct tcggtgccgt tagctttcag ctcgttaatc tgcccgcgcg
    27001 cgtcttcttc ggtaatgtag ctacgttgat cgaggcgcaa attaaaggtt ttttgaaacg
    27061 ccaccagtgc cggaatagtt tcctgataag tgacaacgcc gatagaagag gtgagctttc
    27121 cggcttttgc cagagcctgt aacacatcgt agccgctcgg tttaatcaaa ataaccggca
    27181 ctgacaggcg acttttaaga tatgcgccgt tagagccagc agcgatgatg gcgtcgcagc
    27241 gttcgtttgc cagttttttg cggatgtagg tcaccgcttt ttcaaagcca agctgaatag
    27301 gggtgatatt cgccaggtga tcaaactcga gactgatatc gcgaaacagc tcgaacagcc
    27361 gcgttacaga taccgtccag atcaccggtt tgtcgtcatt cagccgtggt ggatgtgcca
    27421 tagcgcaccg caaagttaag aaaccgaata ttgggtttag tcttgtttca taattgttgc
    27481 aatgaaacgc ggtgaaacat tgcctgaaac gttaactgaa acgcatattt gcgaattact
    27541 tcatgactat attgctaaca aattgaaatt aaacatttaa ttttattcag tcaattgtgg
    27601 cacacccctt gctttgtgtt tatcaacgca aataacaagt tgataacaaa ggatgggcta
    27661 tgtatctaca ctctccaggt aaagcgtttc gcgctgcact gtctaaagaa aatccattgc
    27721 agattgttgg caccatcaac gctaaccatg cgctgctggc gcagcgtgcc ggatatcagg
    27781 caatttatct ctccggcggt ggcgtggcgg cagggtcgct ggggctgccc gatctcggta
    27841 tttctactct tgatgacgtg ctgacagata ttcgccgtat caccgacgtt tgttcgctgc
    27901 cgctgctggt ggatgcggat atcggttttg gttcttctgc ttttaacgtg gcgcgcaccg
    27961 tgaaatcgat gattaaagcc ggtgcggcag gattgcatat tgaagatcag gttggtgcga
    28021 aacgctgcgg tcatcgtccg aataaagcga tcgtctcgaa agaagagatg gtggatcgga
    28081 tccgcgcggc ggtggatgcg aaaaccgatc ctgattttgt gatcatggcg cgcaccgatg
    28141 cgctggcggt agaggggctg gatgcggcga ttgagcgtgc gcaggcctat gttgaagcgg
    28201 gtgccgagat gttgttcccg gaggcaatta ccgaactcgc catgtaccgc cagtttgccg
    28261 atgcggtgca ggtgccgatc ctcgccaaca tcaccgaatt tggcgcaaca ccgctgttta
    28321 ccaccgacga attacgcagc gcccatgtcg caatggcgct gtacccactt tcagcgttcc
    28381 gcgccatgaa ccgcgccgct gagcatgtct acaacgtcct gcgtcaggaa gggacgcaga
    28441 aaagcgtcat cgacaccatg cagacccgca acgagctgta cgaaagcatc aactactacc
    28501 agtacgaaga gaagctcgac gacctgtttg cccgtggtca ggtgaaataa aaacgcccgt
    28561 tggttgtatt cgacaaccga tgcctgatgc gccgctgacg cgacttatca ggcctacgag
    28621 gtgaactgaa ctgtaggtcg gataagacgc atagcgtcgc atccgacaac aatctcgacc
    28681 ctacaaatga taacaatgac gaggataata tgagcgacac aacgatcctg caaaacagta
    28741 cccatgtcat taaaccgaaa aaatcggtgg cgctttctgg cgttccggcg ggcaatacgg
    28801 cgctctgcac cgtgggtaaa agcggcaatg atttgcatta ccgcggctac gacattcttg
    28861 atctggcgga acattgcgaa tttgaagaag tggcgcatct gctgatccac ggcaaactgc
    28921 cgacccgtga cgaactcgcc gcctacaaaa cgaaactgaa agccctgcgc ggtttaccgg
    28981 ctaacgtgcg taccgtgctg gaagccttac cggcggcgtc acacccgatg gatgttatgc
    29041 gcaccggcgt ttccgcgctc ggctgcacgc tgccagaaaa agaggggcat accgtctctg
    29101 gcgcgcggga tattgccgac aaactgctgg cgtcacttag ctcgattctt ctctactggt
    29161 atcactacag ccacaacggc gaacgcattc agccggaaac cgatgacgac tccatcggcg
    29221 gtcacttcct gcatctgctg cacggcgaaa agccgtcgca aagctgggaa aaggcgatgc
    29281 atatttcgct ggtgctgtac gccgaacacg agtttaacgc ctccaccttt accagccggg
    29341 tgattgcggg caccggctct gatatgtatt ccgcgattat tggcgcgatt ggtgcactgc
    29401 gcgggccgaa gcacggcggg gcgaatgaag tgtcgctgga gatccagcaa cgctacgaaa
    29461 cgccggacga agccgaagcc gatatccgca agcgggtgga aaacaaagaa gtggtcattg
    29521 gttttggtca tccggtttat accatcgccg acccgcgtca tcaggtgatc aaacgtgtgg
    29581 cgaagcagct ctcgcaggaa ggcggctcgc tgaagatgta caacatcgcc gatcgcctgg
    29641 aaacggtgat gtgggagagc aaaaagatgt tccccaatct cgactggttc tccgctgttt
    29701 cctacaacat gatgggtgtt cccaccgaga tgttcacacc actgtttgtt atcgcccgcg
    29761 tcactggctg ggcggcgcac attatcgaac aacgtcagga caacaaaatt atccgtcctt
    29821 ccgccaatta tgttggaccg gaagaccgcc agtttgtcgc gctggataag cgccagtaaa
    29881 cctctacgaa taacaataag gaaacgtacc caatgtcagc tcaaatcaac aacatccgcc
    29941 cggaatttga tcgtgaaatc gttgatatcg tcgattacgt gatgaactac gaaatcagct
    30001 ccaaagtagc ctacgacacc gcacattact gcctgctcga cacgctcggc tgcggtcttg
    30061 aagctctcga atacccagcc tgtaaaaaac tgctggggcc aattgtcccc ggcaccgtcg
    30121 tacctaacgg cgtgcgcgtc cccggaactc agttccagct cgaccccgtc caggcggcat
    30181 ttaacatcgg cgcgatgatc cgttggctcg atttcaacga tacctggctg gcggcggagt
    30241 ggggccatcc ttccgacaac ctcggcggca ttctggcaac ggcagactgg ctttcgcgca
    30301 acgcggtcgc tagcggcaaa gcgccgttga ccatgaagca ggtgctgacc gcaatgatca
    30361 aagcccatga aattcagggc tgcatcgcgc tggaaaactc ctttaaccgc gtcggcctcg
    30421 accacgttct gttagtgaaa gtggcttcca ccgccgtggt cgccgaaatg ctcggcctga
    30481 cccgcgagga aattctcaac gccgtttcgc tggcgtgggt ggacggtcag tcgctgcgca
    30541 cctatcgcca tgcgccgaac accggcacgc gtaaatcctg ggcggcgggc gatgccactt
    30601 cccgcgcggt acgtctggca ctgatggcga aaacgggcga aatgggttat ccgtcagccc
    30661 tgactgcgcc agtatggggt ttctacgacg tctcctttaa aggtgagtca ttccgcttcc
    30721 agcgtccgta cggttcctac gtcatggaaa atgtgctgtt caaaatctcc ttcccggcgg
    30781 agttccactc ccagacggca gttgaagcgg cgatgacgct ctatgaacag atgcaggcag
    30841 caggcaaaac ggcggcagat atcgaaaaag tgaccattcg cacccacgaa gcctgtattc
    30901 gcatcatcga caaaaaaggg ccgctcaata acccggcaga ccgcgaccac tgcattcagt
    30961 acatggtggc gatcccgctg ctgttcggac gcttaacggc ggcagattac gaggacaacg
    31021 ttgcgcaaga taaacgcatc gacgccctgc gcgagaagat caattgcttt gaagatccgg
    31081 cgtttaccgc tgactaccac gacccggaaa aacgcgccat cgccaatgcc ataacccttg
    31141 agttcaccga cggcacacga tttgaagaag tggtggtgga gtacccaatt ggtcatgctc
    31201 gccgccgtca ggatggcatt ccgaagctgg tcgataaatt caaaatcaat ctcgcgcgcc
    31261 agttcccgac tcgccagcag cagcgcattc tggaggtttc tctcgacaga actcgcctgg
    31321 aacagatgcc ggtcaatgag tatctcgacc tgtacgtcat ttaagtaaac ggcggtaagg
    31381 cgtaagttca acaggagagc attatgtctt ttagcgaatt ttatcagcgt tcgattaacg
    31441 aaccggagaa gttctgggcc gagcaggccc ggcgtattga ctggcagacg ccctttacgc
    31501 aaacgctcga ccacagcaac ccgccgtttg cccgttggtt ttgtgaaggc cgaaccaact
    31561 tgtgtcacaa cgctatcgat cgctggctgg agaaacagcc agaggcgctg gcattgattg
    31621 ccgtctcttc ggaaacagag gaagagcgta cctttacctt ccgccagtta catgacgaag
    31681 tgaatgcggt ggcgtcaatg ctgcgctcac tgggcgtgca gcgtggcgat cgggtgctgg
    31741 tgtatatgcc gatgattgcc gaagcgcata ttaccctgct ggcctgcgcg cgcattggtg
    31801 ctattcactc ggtggtgttt gggggatttg cttcgcacag cgtggcaacg cgaattgatg
    31861 acgctaaacc ggtgctgatt gtctcggctg atgccggggc gcgcggcggt aaaatcattc
    31921 cctataaaaa attgctcgac gatgcgataa gtcaggcaca gcatcagccg cgtcacgttt
    31981 tactggtgga tcgcgggctg gcgaaaatgg cacgcgtcag cgggcgggat gtcgatttcg
    32041 cgtcgttgcg ccatcaacac atcggcgcgc gggtaccggt ggcgtggctg gaatccaacg
    32101 aaacctcctg cattctctac acctccggca cgaccggcaa acctaaaggc gtgcaacgtg
    32161 acgttggcgg atatgcggtg gcgctggcga cctcgatgga caccattttt ggcggcaaag
    32221 cgggcggtgt gttcttttgt gcttcggata tcggctgggt ggtggggcat tcgtatatcg
    32281 tctacgcgcc gctgctggcg gggatggcga ctatcgttta cgaaggatta ccgacctggc
    32341 cggactgcgg cgtgtggtgg aaaatcgtcg agaaatatca ggttagccgc atgttctcag
    32401 cgccgaccgc cattcgcgtg ctgaaaaaat tccctaccgc tgaaattcgc aaacacgatc
    32461 tctcgtcgct ggaagtgctc tatctggcgg gggagccgct ggacgagcca accgccagtt
    32521 gggtgagcaa tacgctggat gtgccggtga tcgacaatta ctggcagacc gaatccggct
    32581 ggccgattat ggcgattgct cgcggtctgg acgacaggcc gacgcgtctg ggaagccccg
    32641 gcgtgccgat gtatggctat aacgtgcagt tgctcaatga agtcaccggc gaaccgtgcg
    32701 gcgtcaacga gaaggggatg ctggtggtgg aagggccgct gccgccgggg tgtattcaga
    32761 ccatctgggg cgatgacgac cgctttgtga agacgtactg gtcgctgttt tcccgtccgg
    32821 tgtatgccac ctttgactgg ggcatccgcg atgctgacgg ttatcacttt attctcgggc
    32881 gcactgacga tgtgattaac gttgccgggc atcggctggg gacgcgcgag attgaagaga
    32941 gtatctccag tcatccgggc gttgccgaag tggcggtggt tggggtgaaa gatgcgctga
    33001 aagggcaggt ggcggtggcg tttgtcattc cgaaagagag cgacagtctg gaagaccgcg
    33061 aggtggcgca ctcgcaagag aaggcgatta tggcgctggt ggacagccag attggcaact
    33121 ttggccgccc ggcgcacgtc tggtttgtct cgcaattgcc gaaaacgcga tccggaaaaa
    33181 tgctgcgccg cacgatccag gcgatttgcg aaggacgcga tcctggggat ctgacgacca
    33241 ttgatgatcc ggcgtcgttg gatcagatcc gccaggcgat ggaag
//
LOCUS       DAZGQL010000058        32862 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.058, whole genome shotgun sequence.
ACCESSION   DAZGQL010000058 DAZGQL010000000
VERSION     DAZGQL010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32862
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.058"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            188..1405
                     /gene="alaA"
                     /locus_tag="ACHKIR_002964"
     CDS             188..1405
                     /gene="alaA"
                     /locus_tag="ACHKIR_002964"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HFO9990668.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            1489..2088
                     /gene="yfbR"
                     /locus_tag="ACHKIR_002965"
     CDS             1489..2088
                     /gene="yfbR"
                     /locus_tag="ACHKIR_002965"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFO9990669.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(2147..3979)
                     /gene="yfbS"
                     /locus_tag="ACHKIR_002966"
     CDS             complement(2147..3979)
                     /gene="yfbS"
                     /locus_tag="ACHKIR_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFO9990670.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(4066..4716)
                     /gene="hxpA"
                     /locus_tag="ACHKIR_002967"
     CDS             complement(4066..4716)
                     /gene="hxpA"
                     /locus_tag="ACHKIR_002967"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HFO9990671.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(4727..5221)
                     /gene="yfbU"
                     /locus_tag="ACHKIR_002968"
     CDS             complement(4727..5221)
                     /gene="yfbU"
                     /locus_tag="ACHKIR_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFO9990672.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(5305..5760)
                     /gene="yfbV"
                     /locus_tag="ACHKIR_002969"
     CDS             complement(5305..5760)
                     /gene="yfbV"
                     /locus_tag="ACHKIR_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminus macrodomain insulation protein YfbV"
                     /protein_id="HFO9990673.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            6098..7300
                     /gene="ackA"
                     /locus_tag="ACHKIR_002970"
     CDS             6098..7300
                     /gene="ackA"
                     /locus_tag="ACHKIR_002970"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFO9990674.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            7375..9519
                     /gene="pta"
                     /locus_tag="ACHKIR_002971"
     CDS             7375..9519
                     /gene="pta"
                     /locus_tag="ACHKIR_002971"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFO9990675.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIDSLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            9709..11229
                     /gene="yfcC"
                     /locus_tag="ACHKIR_002972"
     CDS             9709..11229
                     /gene="yfcC"
                     /locus_tag="ACHKIR_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFO9990676.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAIFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(11262..11804)
                     /gene="yfcD"
                     /locus_tag="ACHKIR_002973"
     CDS             complement(11262..11804)
                     /gene="yfcD"
                     /locus_tag="ACHKIR_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HFO9990677.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(11862..12413)
                     /gene="yfcE"
                     /locus_tag="ACHKIR_002974"
     CDS             complement(11862..12413)
                     /gene="yfcE"
                     /locus_tag="ACHKIR_002974"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HFO9990678.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(12469..13113)
                     /gene="yfcF"
                     /locus_tag="ACHKIR_002975"
     CDS             complement(12469..13113)
                     /gene="yfcF"
                     /locus_tag="ACHKIR_002975"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HFO9990679.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERSTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            13249..13896
                     /gene="yfcG"
                     /locus_tag="ACHKIR_002976"
     CDS             13249..13896
                     /gene="yfcG"
                     /locus_tag="ACHKIR_002976"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HFO9990680.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAGLDYRLIKVDLGKGGQFRPEFL
                     HISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAASQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            13953..14315
                     /gene="folX"
                     /locus_tag="ACHKIR_002977"
     CDS             13953..14315
                     /gene="folX"
                     /locus_tag="ACHKIR_002977"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HFO9990681.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            14336..15229
                     /gene="yfcH"
                     /locus_tag="ACHKIR_002978"
     CDS             14336..15229
                     /gene="yfcH"
                     /locus_tag="ACHKIR_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HFO9990682.1"
                     /translation="MKIVITGGTGLIGRHLIPRLLELGHQITVMTRNPQKASSVLGPR
                     VALWQGLADQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCAHWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPATAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(15277..16179)
                     /gene="rpnB"
                     /locus_tag="ACHKIR_002979"
     CDS             complement(15277..16179)
                     /gene="rpnB"
                     /locus_tag="ACHKIR_002979"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HFO9990683.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARDFIDIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGAGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEIAERAPQEKEKLMTIADRLREEGRNDGLILGKREEALRIAQEMLE
                     RGLDRELVMMVTRLSPDDLIAQSH"
     gene            complement(16376..17149)
                     /gene="hisP"
                     /locus_tag="ACHKIR_002980"
     CDS             complement(16376..17149)
                     /gene="hisP"
                     /locus_tag="ACHKIR_002980"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HFO9990684.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(17157..17873)
                     /gene="hisM"
                     /locus_tag="ACHKIR_002981"
     CDS             complement(17157..17873)
                     /gene="hisM"
                     /locus_tag="ACHKIR_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO9990685.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(17870..18556)
                     /gene="hisQ"
                     /locus_tag="ACHKIR_002982"
     CDS             complement(17870..18556)
                     /gene="hisQ"
                     /locus_tag="ACHKIR_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HFO9990686.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(18646..19428)
                     /gene="hisJ"
                     /locus_tag="ACHKIR_002983"
     CDS             complement(18646..19428)
                     /gene="hisJ"
                     /locus_tag="ACHKIR_002983"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HFO9990687.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(19649..20431)
                     /gene="argT"
                     /locus_tag="ACHKIR_002984"
     CDS             complement(19649..20431)
                     /gene="argT"
                     /locus_tag="ACHKIR_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HFO9990688.1"
                     /translation="MKKSILALSLLVGLSAVASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(20697..21266)
                     /gene="ubiX"
                     /locus_tag="ACHKIR_002985"
     CDS             complement(20697..21266)
                     /gene="ubiX"
                     /locus_tag="ACHKIR_002985"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HFO9990689.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDIAASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(21361..22878)
                     /gene="purF"
                     /locus_tag="ACHKIR_002986"
     CDS             complement(21361..22878)
                     /gene="purF"
                     /locus_tag="ACHKIR_002986"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFO9990690.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIEAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(22915..23403)
                     /gene="cvpA"
                     /locus_tag="ACHKIR_002987"
     CDS             complement(22915..23403)
                     /gene="cvpA"
                     /locus_tag="ACHKIR_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HFO9990691.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(23662..24312)
                     /gene="dedD"
                     /locus_tag="ACHKIR_002988"
     CDS             complement(23662..24312)
                     /gene="dedD"
                     /locus_tag="ACHKIR_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HFO9990692.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAVNNTEFEPEPA
                     PVAPPKPKPVESPKPKVEAPPASKPVVEEKAAPTGKAYVVQLGALKNADKVNEIVGKL
                     RGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYTPN"
     gene            complement(24302..25570)
                     /gene="folC"
                     /locus_tag="ACHKIR_002989"
     CDS             complement(24302..25570)
                     /gene="folC"
                     /locus_tag="ACHKIR_002989"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HFO9990693.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYTSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKSA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(25640..26554)
                     /gene="accD"
                     /locus_tag="ACHKIR_002990"
     CDS             complement(25640..26554)
                     /gene="accD"
                     /locus_tag="ACHKIR_002990"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFO9990694.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(26710..27369)
                     /gene="dedA"
                     /locus_tag="ACHKIR_002991"
     CDS             complement(26710..27369)
                     /gene="dedA"
                     /locus_tag="ACHKIR_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFO9990695.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGDKLFS
                     NPDSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAFN
                     VIGALLWVLIFTYAGYFFGTLPFIQSNLKLMIVGIIFVSILPGVFEFIRHKRAAARAA
                     K"
     gene            complement(27452..28264)
                     /gene="truA"
                     /locus_tag="ACHKIR_002992"
     CDS             complement(27452..28264)
                     /gene="truA"
                     /locus_tag="ACHKIR_002992"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFO9990696.1"
                     /translation="MSDQQQPPVYKIALGIEYDGSRYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(28264..29277)
                     /gene="usg"
                     /locus_tag="ACHKIR_002993"
     CDS             complement(28264..29277)
                     /gene="usg"
                     /locus_tag="ACHKIR_002993"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFO9990697.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(29343..30500)
                     /gene="pdxB"
                     /locus_tag="ACHKIR_002994"
     CDS             complement(29343..30500)
                     /gene="pdxB"
                     /locus_tag="ACHKIR_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020218743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033711 - 4-phosphoerythronate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HFO9990698.1"
                     /translation="MSRTEKLLTILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLAD
                     ADALMVRSVTKVNESLLAGKPIKFVGTATAGTDHVDDTWLKQAGIGFSAAPGCNAIAV
                     VEYVFSSLLMLAERDGFSLHERTVGIVGVGNVGRRLQARLEALGITTLLCDPPRADRG
                     DEGDFRSLDELVQHADILTFHTPLFKDGPYKTRHLADEKLIRSLKPGAILINACRGAV
                     VDNTALLTCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQ
                     VFEAYSKFIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLR
                     KVAGIPGEFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            complement(30660..31664)
                     /locus_tag="ACHKIR_002995"
     CDS             complement(30660..31664)
                     /locus_tag="ACHKIR_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO9990699.1"
                     /translation="MSIKKLEDGRYLLDIRPNGRKGKRVRKVFNKKSVAVATERYIMA
                     NAEKREYIQGYRDRRTLNDLLELWWVYHGQHRRKAEEDRKQLRNIINELGADMQAVDL
                     DKLKIIAWRSRRIADGLKPSSANRYMNRLSGMFTALKKIGLWDAEHPVRGISVLYVSP
                     REMAFLSQKEVALLLDTLEGDYWRVALLCLSTGARWGEASKLRGEQIVHNRVTFLETK
                     NGRKRTVPISQAVCEAIKTSETGGLFDVKYREFCLVLKRVKPDLPKGQAAHVLRHTFA
                     SHFVMNGGNIIALQRILGHATIQQTMAYAHFAPDYLQDAVALNPLKGGVSVHSVSTGE
                     "
     gene            complement(31761..32090)
                     /locus_tag="ACHKIR_002996"
     CDS             complement(31761..32090)
                     /locus_tag="ACHKIR_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO9990700.1"
                     /translation="MPDMASLSSMGEKFRLIREAEGMTRQEFADCVGLPYGTVTNYEV
                     RGKQVTEGALLKVTKHPKFKKYAYWLSTDETMPEAGQIAPALSLDGSCNLEGNQDSTE
                     TTRKLPR"
     gene            32195..32482
                     /locus_tag="ACHKIR_002997"
     CDS             32195..32482
                     /locus_tag="ACHKIR_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cox family DNA-binding protein"
                     /protein_id="HFO9990701.1"
                     /translation="MRESELEGFIQVAPYPLEAVPYQLFAKMIGRKESTVRTMIDAAK
                     LPTIDFVKPGSVKTRASENWVYLPAFNAGMRKAFFNQPKEQRDAWLLWLGL"
     gene            32678..>32862
                     /locus_tag="ACHKIR_002998"
     CDS             32678..>32862
                     /locus_tag="ACHKIR_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000194242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4761 family protein"
                     /protein_id="HFO9990702.1"
                     /translation="MTQRRSFRTGTERHAARFSTSASRSNTHYSLQETHATPDGHLVK
                     QTGKHTWLINDAGIVIH"
BASE COUNT         8207 a         8949 c         7936 g         7770 t
ORIGIN      
        1 cattaattac taagcatata cacctcatta ttttgccatt attaagttat taacagcaca
       61 atcgagtctt cccctctggc aaaatcttat tctgcagacc ttcaaaacgc cgtcctgggg
      121 gagtacattg ttctaagctg acttccacgg cagggagtgg cgataacagc aaaaaaggtc
      181 aagattcatg tcccccattg aaaaatccag caaattagag aatgtctgtt acgacatccg
      241 tggtccggtg ctgaaagaag caaaacgcct ggaagaagaa ggtaacaagg tactgaaact
      301 gaacatcggc aaccctgccc cgttcggttt tgacgcgcca gatgaaatcc tcgttgacgt
      361 gattcgcaac ctgcctaccg ctcaagggta ttgcgattcc aaagggcttt actccgcgcg
      421 taaagccatc atgcagcact accaggctcg tggcatgcgt gatgttaccg tggaagatat
      481 ttacatcgga aatggtgtat cggagcttat cgttcaggca atgcaggcat tgctgaacag
      541 cggcgacgaa atgttggttc ctgcacctga ttacccacta tggaccgcgg cggtttcgct
      601 ctccagcggt aaagcggtgc attatctttg cgatgaatcc tctgactggt tcccggacct
      661 tgatgatatt cgcgctaaaa ttacgcctcg tacgcgtggg atcgttatta tcaacccaaa
      721 taacccaacc ggcgcggtat attccaaaga gcttttaatg gagatagtgg agattgcacg
      781 tcagcataat ctcattatct tcgccgatga aatttatgac aaaattctct acgacgacgc
      841 tgagcatcac tcaattgcgc cgctggcacc tgatctgctg accattacct ttaacggcct
      901 gtcgaaaaca taccgcgttg caggcttccg tcaggggtgg atggtgttga acgggccgaa
      961 aaaacacgcc aaaggctaca tcgaaggtct ggaaatgctg gcttcaatgc gcctgtgtgc
     1021 taacgttcct gcgcaacacg ccattcagac cgcgctgggc ggttatcaga gcatcagtga
     1081 atttattacc cctggcggtc gtctttatga gcagcgtaac cgcgcatggg aattgatcaa
     1141 tgatattccg ggcgtttcct gcgtgaaacc tcgtggtgcg ctgtacatgt tcccgaaaat
     1201 cgacgccaaa cgctttaata ttcacgacga tcagaaaatg gtgctggatt tcctgttgca
     1261 ggaaaaagtt ctgttggtgc aagggacggc attcaactgg ccgtggccgg atcacttccg
     1321 cattgtcacg ctaccgcgtg tcgatgatat cgagctgtct ttgagcaagt tcgcgcgttt
     1381 cctttctggt tatcatcagc tgtaatctta atttcactgc cggagattgc atccggcagt
     1441 gttatcccgc cacaatgacc tgatgatgtc atcatacgta aggtcactat gaaacagagc
     1501 catttttttg cccatctctc ccgcctgaaa ctcattaacc gctggccgct aatgcgcaat
     1561 gtgcggacgg aaaatgtgtc cgaacacagt ttgcaggtag cgatggtcgc ccatgcgctg
     1621 gcagctatca aaaatcgtaa atttggcggt aatgtcaacg ccgaacgtat cgctttactg
     1681 gcgatgtacc acgatgcctc agaagtgctt accggcgatc tccctacccc ggtgaaatac
     1741 ttcaattcgc aaatcgctca ggaatacaag gcgattgaaa aaatcgctca gcaaaaactg
     1801 gtcgatatgg ttccggaaga gttgcgggat atctttgcgc cgttaattga cgagcatgca
     1861 tatagtgatg aagaaaaatc actggtgaaa caggcagatg cactatgtgc atatctgaaa
     1921 tgtctggaag aactcgcggc gggaaataat gaattcttgc tggcaaaaac gcgactggaa
     1981 gcgacgcttg aagcgcgccg cagccaggag atggactact tcatggaagt ctttgttccc
     2041 agcttccatc tttcgctcga tgagattagc caggattcac cgctgtaagc agccggagtc
     2101 tgcgtcgcat caggcaataa gcgccggatg cgacatcagg ctcttgtcaa aacggaaaca
     2161 gcatcgggat catcaccaca caaaccgcca tcacgataat ggtgaacggt acccccaact
     2221 tcacaaagtc actgaagctg taatttcccg gacctaaaac cagtgtgtta acaggtgaag
     2281 aaaccggcgt cataaaggcg gcggatgctg ccatcgccac gaccatcgcg aatgggtaag
     2341 gcgacacccc catcgtttta gcagcagcca gcgcaatcgg agccatcaac accgccgtcg
     2401 cggtattaga aataaatagc ccaataaccg ccgacaagac aaacaaacaa cccagcatca
     2461 tatgtggccc gtaaccgccg ccaatgtcca tcagcccttt caccgccagc gcaacacctc
     2521 ccgttttctg taatgccaca gcaaacggca tcatcccaac gatcaaaata atgctcggcc
     2581 agtgtatgga tttataggcg ctttcagcat cgatacagcg gaatttcccc atcagcaggc
     2641 aggcgatgat agcggcaaca ggattaggaa tttcatcggt cagcattaac gccaccatta
     2701 gcaccagaca gaaaatggca tggggtgcct gactgtgcgc gggcgatgct tcactcacct
     2761 cttccggtaa gttcagcgct acgaagtcgc ggccctgttt ggccagcata ccgatcagtt
     2821 tccagttacc cacaaccagg atgatatcgc ccagcagcag aggctcatcc gccagcgaac
     2881 cttccagcgc cacgccattg cgctttagcc ccactacatt caatccgtag cgggtacgaa
     2941 aaccaatttc gcgcaccgat ttaccaataa gttctgactc aggaattaat gaaatctctg
     3001 ccatgcccac atcaagggcc tggtcagaaa aatactcgcc gcgcagtacc atcggctcca
     3061 gcaattgctc actacaaaat tgccggagat cgacatcagc cgcagacata tcaataagca
     3121 aaacgtcacg cgcgcgaaat tcagaaaccc cattaacgtt cacgataacg cgacgaaaac
     3181 gccgccagcg ttcaacaccg atgacgttag caccataacg ttcacgtaat ttgagatcgt
     3241 ccagtcgttg accaatcatt ggcgatccgg ggcgaatagc cagacgtcgc gcacgcccgg
     3301 tcagtcgata ttcacggata agatcgcgaa aggttcggcg cgtccagcct tcgcgttgtg
     3361 gggtctgggt atcccctttc agcatgaaac gcatcactaa catatacaaa atacccagca
     3421 ccagcacgac caggccaata ggtgttacgc taaagaaact gaagccgtga tagccttcac
     3481 gcagcaattc actgttgact accaggttcg gcggcgtcgc caccagcgtc atcatgccgc
     3541 ttatcagccc ggcaaaactt aacggcatca tcagacgcga cggcgacgtt tgcatacgca
     3601 tggcaacgct taacaccacg ggaataaaga tagcgacaac gccggttgaa ctcataaacg
     3661 cgccaagccc cgcgacggtc agcatcaaca aaaccaacat tttgatttca ctgttgcccg
     3721 ccactttgac cagccatgtc cccattacag tggcaacacc ggtacggacc aaaccatcgc
     3781 caataatgaa caaggcggca atcaggacaa cgttaggatc ggaaaagccg gaaaatactt
     3841 cggggaccgt cagcgttccg cttaatgcaa acgcgacaat aacaaacaaa gcgaccgcat
     3901 ccatacgcac tctgcccgtc gcaaacaaga cgatggcaac ggccagtaat gaaagaaccc
     3961 aaatcaattc accgttcaca acttatcctt gttaattgag ggggataact tgattctgcc
     4021 ataaaaaagc cccgacgaga cggggctaaa tcatgatcag gtgtttcact gaataataac
     4081 atcgccattt ggctgtttgg tcacagtaat ttgctccaga ctgtggagga ccaaatcgac
     4141 ctcattcagg cgcggggtat ctgccggagc gttaaccgca atgacatggc aacccgccgc
     4201 caggccagaa agcacgccag cgggagcatc ttccaccact acacactcct gcggcgcaag
     4261 ccccagcagc tgcgcgccta gcagatacgc atcaggttct ggttttccgc gcttcactcg
     4321 ctcagcggtc acaaacacct ctggcgcggg aagcccagct attttatggc gcgctcgcgc
     4381 taccggcatg gagccagaag tcacaatggc ccacggaata cctgctttat tcaaatgatt
     4441 gagtaaggcg attgcccccg gaagtgcggt aataccctcg gtttccgtgg cctcgatttg
     4501 ctccagacgc gtaaactcgg cggcaatatc agcctcggat ttgcccgcca taaaatggcg
     4561 caaagaggtg atcgcctgtt taccgtgaat aaaagccagc acctcttccg gcgctaaccc
     4621 atgacgtctg gcccagttgc tccacgcccg ttctaccgca ggcagggaat ccaccagcgt
     4681 tccatcaaga tcaaacagaa aacctttgca ccgcacgcgg gcctcctcag gcgttgataa
     4741 tttgattaat ttcgttggcg ctcaaatggt actgacgcgg gcaggcatgc cacacattaa
     4801 gcatgcgctg atatttttcc cacattggcg tctgggcgtt aaaaccgtga gttccggcat
     4861 caaaatgggt atagcgtcct tccacattaa ccataaagcg gacataaccg aggtaacgtg
     4921 cttcagtggc ggcgtcaaag ccgaggaagg tgacacgacg ttcatcgatg gattgctggt
     4981 cctgcaaatt agaccaggaa acatgcaacg catgatacat ctccataatg tcgatgatag
     5041 tgcggcaggt ttcttctttc agctcgccaa actcgcgatc cagttcacgc atctgtaatc
     5101 cataaccacg ctcaatgatt gtttgcaggc gacggtaacg ttcagcattt gccggatcga
     5161 gcatagtcat catcttgtac tggttagaca aaataagacg ttgcgcgttg gtcatttcca
     5221 ttgttgactc ctgtatcact ctactacggt gaaaaaaaaa gaaggctgag tatgccttct
     5281 tttatatgcg taatcagggg tcaattacaa atcatcaagg aaagttttat ccagttgttt
     5341 gaaggcgcgc ttaagcgtgt cagctaatgc ctggtaatca ggcttgcctt caacgggtgc
     5401 caacacctgt ccagactcct gcaatttacc gcgaacttca taaaaccagt taaggattgc
     5461 agggggtaat ggcgttacag aacgtttgcc cagccaccac aatccctgca tgggtaaact
     5521 taaggcgaac agcgcagtgg caactgccgg cccaagctga ccgcccaggg caatctgcca
     5581 gcagagagta aatacggcga tcggcggcat aaaacggatc gcataacgcg tcatcttgat
     5641 aacgcgattt tcgacaaaga ccggggcaag gcgtttttcc agcggccacg tctttgagta
     5701 atgctgtccc cggcgaaaca agctaaaaaa attaacagaa cgattatccg gcgttgacat
     5761 gcttcacctc aacttcacat ataaagattc aaaaatttgt gcaaattcac aactcagcgg
     5821 gacaacgttc aaaacatttt gtcttccata cccactatca ggtatccttt agcagcctga
     5881 aggcctaagt agtacatatt cattgagtcg tcaaattcat atacattatg ccattggctg
     5941 aaaattacgc aaaatggcat agactcaaga tatttcttcc atcatgcaaa aaaaatttgc
     6001 agtgcatgat gttaatcata aatgtcggtg tcatcatgcg ctacgctcta tggctccctg
     6061 acgttttttt agccacgtat caattatagg tacttccatg tcgagtaagt tagtactggt
     6121 tctgaactgc ggtagttctt cactgaaatt tgccatcatc gatgcagtaa atggtgaaga
     6181 gtacctttct ggtttagccg aatgtttcca cctgcccgaa gcacgtatca aatggaaaat
     6241 ggacggcaat aaacaggaag cggctttagg tgcaggcgcc gctcacagcg aagcgctcaa
     6301 ctttatcgtt aatactattc tggcacaaaa accagaactg tctgcgcagc tgactgctat
     6361 cggtcaccgt atcgtacacg gcggcgaaaa gtataccagc tccgtagtga tcgatgagtc
     6421 tgttattcag ggtatcaaag atgcagcttc ttttgcaccg ctgcacaacc cggctcacct
     6481 gatcggtatc gaagaagctc tgaaatcttt cccacagctg aaagacaaaa acgttgctgt
     6541 atttgacacc gcgttccacc agactatgcc ggaagagtct tacctctacg ccctgccgta
     6601 caacctgtac aaagagcacg gcatccgtcg ttacggcgcg cacggcacca gccacttcta
     6661 tgtaacccag gaagcggcaa aaatgctgaa caaaccggta gaagaactga acatcatcac
     6721 ctgccacctg ggcaacggtg gttccgtttc tgctatccgc aacggtaaat gcgttgacac
     6781 ctctatgggc ctgaccccgc tggaaggtct ggtcatgggt acccgttctg gtgatatcga
     6841 tccggcgatc atcttccacc tgcacgacac cctgggcatg agcgttgacg caatcaacaa
     6901 actgctgacc aaagagtctg gcctgctggg tctgaccgaa gtgaccagcg actgccgcta
     6961 tgttgaagac aactacgcga cgaaagaaga cgcgaagcgc gcaatggacg tttactgcca
     7021 ccgcctggcg aaatacatcg gtgcctacac tgcgctgatg gatggtcgtc tggacgctgt
     7081 tgtattcacc ggtggtatcg gtgaaaatgc cgcgatggtt cgtgaactgt ctctgggcaa
     7141 actgggcgtg ctgggctttg aagttgatca tgaacgcaac ctggctgcac gtttcggcaa
     7201 atctggtttc atcaacaaag aaggtacccg tcctgcggtg gttatcccaa ccaacgaaga
     7261 actggttatc gcgcaagacg cgagccgcct gactgcctga tttcacaccg ccagctcagc
     7321 tggcggtgct gttttgtaac ccgccaaatc ggcggtaacg aaagaggata aaccgtgtcc
     7381 cgtattatta tgctgatccc taccggaacc agcgtcggtc tgaccagcgt cagccttggc
     7441 gtgatccgtg caatggaacg caaaggcgtt cgtctgagcg ttttcaaacc tatcgctcag
     7501 ccgcgtaccg gtggcgatgc gcccgatcag actacgacta tcgtgcgtgc gaactcttcc
     7561 accacgacgg ccgctgaacc gctgaaaatg agctacgttg aaggtctgct ttccagcaat
     7621 cagaaagatg tgctgatgga agagatcgtc gcaaactacc acgctaacac caaagacgct
     7681 gaagtcgttc tggttgaagg tctggtcccg acacgtaagc accagtttgc ccagtctctg
     7741 aactacgaaa tcgctaaaac gctgaatgcg gaaatcgtct tcgttatgtc tcagggcact
     7801 gacaccccgg aacagctgaa agagcgtatt gaactgaccc gcaacagctt cggcggtgcc
     7861 aaaaacacca acatcaccgg cgttatcgtt aacaaactga atgcaccggt tgatgaacag
     7921 ggtcgtactc gcccggatct gtccgagatt ttcgacgact cttccaaagc taaagtaaac
     7981 aatgttgatc cggcgaagct gcaagaatcc agcccgctgc cggttctcgg cgcggtgccg
     8041 tggagctttg acctgatcgc gactcgtgcg atcgatatgg ctcgccacct gaatgcaacc
     8101 atcatcaacg aaggcgacat caatactcgc cgcgttaaat ccgtcacttt ctgcgcacgc
     8161 agcattccac acatgctgga gcacttccgt gccggttctc tgctggtgac ttccgcagac
     8221 cgtcctgacg tgctggttgc cgcgtgcctg gctgccatga acggcgtaga aatcggtgcc
     8281 ctgctgctga ctggcggcta cgaaatggac gcgcgcattt ctaaactgtg cgaacgtgct
     8341 ttcgctaccg gcctgccggt atttatggtg aacaccaaca cctggcagac ttctctgagc
     8401 ctgcagagct tcaacctgga agttccggtt gacgatcacg agcgtatcga gaaagttcag
     8461 gaatacgttg ctaactacat caacgctgac tggatcgatt ctctgactgc cacttctgag
     8521 cgcagccgtc gtctgtctcc gccagcgttc cgttatcagc tgactgaact tgcgcgcaaa
     8581 gcgggcaaac gtatcgttct gccggaaggt gacgaaccgc gtaccgttaa ggcagccgct
     8641 atctgtgctg aacgtggtat cgcaacttgc gtactgctgg gtaatccggc agagatcaac
     8701 cgtgttgcag cgtctcaggg tgtagaactg ggtgcaggca ttgaaatcgt tgatccagaa
     8761 gtggttcgcg aaagctatgt tggtcgtctg gtcgagctgc gtaagaacaa aggcatgacc
     8821 gaaaccgttg cccgcgaaca gctggaagat aacgtggttc tcggtacgct gatgctggaa
     8881 caggatgaag ttgatggtct ggtttccggt gctgttcaca ctaccgcaaa caccatccgt
     8941 ccgccgctgc agctgatcaa aactgcaccg ggcagctccc tggtatcttc cgtgttcttc
     9001 atgctgctgc cggaacaggt ttacgtttac ggtgactgtg cgatcaaccc ggatccgacc
     9061 gcagaacagc tggcagaaat cgcgattcag tccgcagatt ccgctgcggc cttcggtatc
     9121 gaaccgcgcg ttgctatgct ctcctactcc accgggactt ctggtgctgg tagcgacgta
     9181 gaaaaagttc gcgaagcaac tcgtctggcg caggaaaaac gtcctgacct gatgatcgac
     9241 ggtccgctgc agtacgacgc tgcggtaatg gctgacgttg cgaaatccaa agcaccgaac
     9301 tctccggttg caggtcgcgc taccgtgttc atcttcccgg atctgaacac cggtaacacc
     9361 acctacaaag cggtacagcg ttctgctgac ctgatctcta tcggaccgat gctgcagggt
     9421 atgcgcaagc cggttaacga cctgtcccgt ggcgcactgg ttgacgatat cgtctacacc
     9481 atcgcgctga ctgcgattca gtctgcacag cagcagtaat ctcgtcatca tccgcagctt
     9541 tacgctgcgg atatctgaac cggaaatagt cactatttcc ggttttttat tctcttaatt
     9601 tgcattaatc ctttctgatt atcttgctta actgcgctgc atcaatgaat tgcgccatct
     9661 caatttgcat acttaccact ttgttttgtg caagggaata tttgcgctat gtccgcaatc
     9721 actgaatcta aaccaacaag aagatgggca atgcccgata cgttggtgat tatctttttt
     9781 gttgccattt taaccagcct tgccacctgg gtagttccgg tgggcatgtt tgacagtcag
     9841 gaagtgcagt atcaggttga tggtcaaaca aaaacacgca aagtcgtaga tccacactca
     9901 tttcgcattc tgactaacga agcaggcgaa cctgagtatc accgcgtaca gctgttcacg
     9961 acgggcgatg aacgcccggg tctgatgaac ttcccgtttg aagggttaac ctcaggatcg
    10021 aaatacggga cagccgttgg catcatcatg tttatgctgg tgattggcgg cgcgtttggc
    10081 attgtgatgc gtacaggaac cattgataac ggtatcctgg cgcttattcg ccatacccgc
    10141 ggaaatgaaa ttctctttat tcctgcgctg tttattctgt tttcacttgg cggcgcaata
    10201 tttggtatgg gagaagaggc cgtcgccttt gccattatta tcgcaccgct aatggtccgg
    10261 ctgggctatg acagtattac caccgtcctg gtgacctata tcgccacgca aatcggtttt
    10321 gccagttcgt ggatgaaccc gttttgtgtg gttgttgctc aggggattgc cggcgttccg
    10381 gtgctttctg gctccgggtt gcgcatcgtg gtgtgggtta tcgccaccct gattggcctg
    10441 atctttacca tggtgtacgc ctcacgagtg aaaaagaatc ctcttctgtc acgcgtgcat
    10501 gagtccgacc gcttctttcg tgaaaagcag gcggatgttg aacaacgtcc gtttaccttt
    10561 ggtgactggc tggtattgat tgtcctgacc gccgtaatgg tctgggtgat ttggggcgtg
    10621 atcgttaatg cctggtttat tccagaaatt gccagccagt tcttcaccat gggtctggtg
    10681 attggcatca tcggcgttgt tttccgcctt aacggcatga cggttaatac catggcttca
    10741 tcctttaccg aaggggcgcg aatgatgatc gcccctgccc tgctggtggg tttcgccaaa
    10801 gggattttgc tgctggtcgg taatggtgaa gcgggtgatg ccagcgtgtt aaataccatc
    10861 ctcaacagca ttgccaatgc cattagcggt ctggataacg cggtcgcggc ctggtttatg
    10921 ttgctcttcc aggcggtatt taatttcttc gtgacatctg gttctggtca ggcggcgtta
    10981 accatgccgt tactggcacc gcttggcgat ctggtcggtg taaaccgtca ggttaccgtt
    11041 ctggcgttcc agtttggtga tggcttcagc cacatcattt acccgacctc agcttcgtta
    11101 atggcaacgc tcggtgtttg ccgggtggac ttccgtaact ggctgaaggt gggcgcgaca
    11161 ctgcttggac tgctgtttat tatgtccagc gtcgtggtga tcggcgctca gttgatgggc
    11221 taccactaaa aatgttaaga gccgcaattg cggctctttt ttcattctgc cgtttcagtc
    11281 tctacggctt catttttggc attgcgcttc atccacaacg ccagcgcttt cagcgagtct
    11341 ggagtgaact catcgcagcg tgcggtgatt tcttccggcg tcagccagca aacttcgctg
    11401 acttcatctt cctgtagggc gaagggaccg tgagagacgc agctgaacaa cgcgccccag
    11461 acacggcaat ttttatcttc gaaatagaac tgcccgtgct cggcaaaggg gacaccggca
    11521 atgcccaact cttcttccgc ttcgcgacgc gcggattcca gcagttgctc atcggcctgg
    11581 actacaccgc ctgcggtcgc atctaacatg ccgggtaaaa agtcttttgt ctcggtacga
    11641 cgctggacca gaattttgcc catgccatca tgcacgacga tgtaagttgc acgatgacgc
    11701 agacactgtg cccgcatttg ttcccggctg gcttgtgcaa tgacttcgtt ctcttcattg
    11761 acaatatcca cccattcagt acttgccaaa cgacgctgtt ccattatcag gaaaccttct
    11821 ttttctggcg ctcttacggc gcattttgag ttgtgggtaa attacggatt aatcgcgacc
    11881 tgcgcaatga tactttgatc attgagtgcg attacactaa gtacgtcatt atccagcatg
    11941 ccataactcg ccggattacc gcctttagga atactcaccg agccggggtt gaagtggaaa
    12001 atctcccccc gctgttctgc cactggcaga tgggtatgac cgtacaccag cacatcgttc
    12061 tggtttaaag caggtaggtt ttccgggcca aacagatgac catgcgtcaa aaacagacgt
    12121 tgtttttcca gtaatacctg ttgccacggc gcggttatcg ggaaatgcaa aagcatttga
    12181 tccacttcgc tgtcgcaatt gccgcgcaca gcgataacct tatgtgccac ttcattaagc
    12241 cgttcagcga ctttggctgg cgcgtaaccc tccggtaaag cattacgcgg gccgtgattc
    12301 aacacgtcgc caaggatcac cagccactgg gcaccacttt gggcaaacaa ctccagaaca
    12361 cgttccgtcg ccggtaacga cccatgaatg tccgatgcaa acatcagttt catcactcac
    12421 tcctcgtcga aaaaacgtaa gctatgatac tggattctgt cgttgctatc agccagattg
    12481 cttcgccgaa agagcaataa aacgctggac agacgctcgc tgccactgga atgttgcata
    12541 atccaccagg cgttctggca cctcatcgcc atgtagtacc aggcggttaa tcattagcgc
    12601 cagatcagta tcagcaatgc accattcacc aaataaattc ggctgaccca gtgctaacaa
    12661 atgttctgcc atcgcgaaca gtttctctgc actggctttt ccctccgccg ttaatggcgc
    12721 ttttttcgca cccgcaaaaa cgacatctgt cgaacgttct tcgcggatgg gcatcagatc
    12781 gctacgcagc caggcctgaa tctgtcgtgc acgcgcacga ttttctaaat caagcggata
    12841 aatgcgttcc caggtcggtg gcgcaaatcg atcttccaga tactccgcaa tggcagaaga
    12901 ttcactcaac tcaaaatcat cgatttgtaa taacggcaca cggcgtgtct gaccgtaacc
    12961 ttgccacgtc ggctgcaaat gttcaccgct gtcgaggtcg atggtcttga tatgaaacga
    13021 caggcctttt tcctgcaacg ccacccaggc ggataacaca taaggggaga aaaaatgggc
    13081 atctgaccaa agcgtgatag cgggtttact cattatgacc tcggcaataa agggggctgg
    13141 ctatcaaact atagagcctt tttgtcactg tcacgtgacg aatactcacg caagtcgccg
    13201 cgctttctat acttgtggtg ttcagatgat tagacaaccg gagctgcaat gatcgatctc
    13261 tatttcgccc cgacaccaaa tggtcacaaa attacgctgt ttctcgaaga agcagggctg
    13321 gattatcgct tgataaaggt agacctgggg aaaggcggtc agtttcgccc ggaatttttg
    13381 cacatttcgc ctaacaacaa aattccggca attgttgatc attctccagc cgatggcggc
    13441 gaaccgctaa gcctctttga gtctggtgcc attttgttgt atctggctga gaaaacagga
    13501 ctctttttga gtcatgaaac gcgtgagcgc gccgccacat tacagtggtt attctggcag
    13561 gtaggcggac tggggccgat gcttgggcaa aatcatcatt ttaatcacgc agcctcccaa
    13621 accattcctt acgctattga acgttatcag gttgaaactc agcgtctgta ccatgtactg
    13681 aacaagcggc tggaaaactc gccctggctg ggtggcgaga actacagcat tgcggatatt
    13741 gcctgctggc cgtgggttaa tgcctggact cgccagcgaa ttgacctcgc aatgtatccg
    13801 gcagtcaaga actggcatga gcggatccgt tcgcgccctg ccaccggtca ggcactgtta
    13861 aaagcacaac tcggtgatga gcgttcggat agttaacaga aacaggttct cgtgtattat
    13921 ttcatcctaa gaaaaacaac ggagaacctg caatggcaca acctgccgct attattcgta
    13981 taaagaacct tcgtttacgt acgtttatcg gaattaagga agaagaaatt aacaaccgtc
    14041 aggatattgt tatcaatgtg acgattcact accccgccga taaagcgcgc accagcgaag
    14101 atatcaacga tgcgctgaat tatcgcaccg tgacgaaaaa cattattcag catgtagaga
    14161 ataaccgttt ctctttgctg gaaaaattaa ctcaggatgt gctcgatatc gcacgtgaac
    14221 atcactgggt gacgtatgct gaagtggaga tcgataaact gcacgcgctg cgctacgccg
    14281 attcggtatc catgacctta agctggcagc gttaaccgcc atatcgggag gctgcatgaa
    14341 gatagtgatc accggaggga caggattaat tggtcgccat ttgattccac gtttgctgga
    14401 gctgggccat caaatcacgg taatgacgcg taacccgcag aaagccagtt ctgttctcgg
    14461 ccctcgggtg gcactatggc aagggcttgc cgatcaaagc aacctcaacg gcgttgatgc
    14521 ggtaatcaac ctggccggag aaccgattgc tgataaacgc tggactcacg agcaaaaaga
    14581 gcgtctctgc caaagccgct ggaatatcac gcaaaaactg gtcgatttga ttaatgccag
    14641 cgacacgccg ccgtcggtac tcatttccgg ctcggcaacg ggctattatg gcgacttagg
    14701 tgaagtggtg gttaccgaag aggaaccgcc gcataacgaa tttactcata aactctgcgc
    14761 ccactgggaa gaaattgcct gccgggcgca aagtgacaaa acgcgagtgt gcctgctgcg
    14821 taccggcgta gtgctggcac cggatggcgg tattctcggt aaaatgctgc cgccgtttcg
    14881 tcttggcctg ggcgggccga ttggttccgg tcggcagtat ctggcctgga ttcatattga
    14941 tgatatggtc aacggcattc tctggctgct ggataacgag ctgcgcgggc catttaatat
    15001 ggtttcgccc tacccggtac gcaatgaaca atttgcccat gcgctcggtc atgctctgca
    15061 tcgcccggct attttgcgcg tccccgcaac cgccattcgg ctgttaatgg gcgaatcttc
    15121 agtactggta ttaggtggac aacgcgcgct gcctaaacgg ctggaagaag cgggttttgc
    15181 gtttcgttgg tacgatttag aagaggcgct ggcggatgtc gttcgctgat gtggtttact
    15241 gcaaacatcc gccagttaac tcccggtgtt acagggttag tggctttgcg cgataagatc
    15301 gtctggtgaa agtcgggtca ccatcataac taactctcgg tctaaacctc tctccagcat
    15361 ctcctgagca atacgcaggg cttcttcgcg tttgccaaga attaatccat cattccgccc
    15421 ttcttcacgt aatctgtcag caatagtcat cagtttctcc ttttcttgtg gtgcgcgttc
    15481 cgctatctca ccaataaatg cacggaaacg ctgggcatcc cccgtttgta atacgtaatt
    15541 aaacagggct tttagctgtc tgtcattagt gttccctgta actagcagcg aaacaatttg
    15601 gtcgactaat cccaacagat cgcgctgacg aatatgtttc tgaattaact ccaacagcgc
    15661 cattttgcga tgttgcataa tctcgtcatc cggcaccacg gtaatatcta ccagcggaaa
    15721 agccgatgaa taaattttgc gggctatagc aggctcggca aattcatcaa gccagcaaag
    15781 tgaataagga taaggacttc tgcaaccatg ataaaacagc atcgggatca ccaatggaag
    15841 ctctttatag cctgcatcaa gatggttttg catcgccgca atggaatagc gcatcatgcg
    15901 aaaagccatt aattcttccg gcttactttg gtgctctatc actacataaa tataacctgc
    15961 tccctcctgc gttttcacag accacaagag gtcggaataa tattgccgca ggtcttcatc
    16021 aataaaactg tttggttcca gttttagcgt tgttaaatca cacagtttgc gcagcggcgc
    16081 gggaagatga atatcaataa aatcccgcgc ggtgtctgga tggcgtaaaa aagatttaaa
    16141 taccgcatca tgcggcgtgg aagttgtcga tattgtcatg gcgttccgtc acccctcaaa
    16201 tttaagatga cgcgacaata accacgatta atacccaatg cagcaggcag tttttatctt
    16261 tggacagcga attcagaact atttttagcg gactgcaact tttacatttc agcggaagct
    16321 gactcgcaaa ttccccttga aaatactccg gcagcgttag ccaccggagg gggaattatt
    16381 tcagcgatcc cttaaggaac tgttgcagac gagggctttg cgggttacca aataactgtt
    16441 ctggcgcgcc ctcttcttct attttcccct gatggaggaa aatgacgtga gtagaaacat
    16501 ggcgagcaaa gcccatttcg tgagtcacta ccaccatggt tttcccctct tctgccagtt
    16561 gctgcataat acgcaacact tcgcctacca gttcaggatc gagcgccgag gtaggttcat
    16621 caaacagtaa aacttccggt tccatcgcca gcgcccgcgc gatagaaaca cgctgttgct
    16681 gaccaccgga caggtgcacc gggtatttcc cctgcgcacg ttcgtctatc ccgacttttg
    16741 ccagatattt caccgcccgc tcgcgcgctt cctgcttgct caggcccaac acctgaatcg
    16801 gcgcttccat gacgttttcc agcaccgtca tatggctcca gagattgaag tgctggaata
    16861 ccatcgtcag gcgtgtgcgc agtaagcgca gctgattttt atcggcgact ttgagttgac
    16921 catctttgtc gcgcaccaga ttgatcgtct ggccattgac cacgatcgat ccttcgctcg
    16981 gtttttcgag gaagttaatg cagcgcagaa aggtactttt ccccgatccc gacgatccga
    17041 tgatgctaat tacatctccg gcattcgctt gcagtgaaac ccctttcagc acttcgtgtt
    17101 cgccgtagcg tttatgcaaa tcgataacgt ttaatttatt ctcggacatc gtattctcag
    17161 tgcgttgaag aaggtttcac atgctgcaac cagcgttttt ccgctctgcg aaagaggctg
    17221 atcaggacat aagagatgat taaatagagc accgcggcaa tgccgaaggc ggtaaaaggt
    17281 tgatatgtgg cggcgttaat atcgcgggca attttcagca gatccggcac cgtggcagta
    17341 aatgccagcg cagtagagtg cagcatcagg atcacttcgt tgctgtacgc cggtaacgca
    17401 atacgcagcg cagaaggcaa aataatgcag cgatacattt taaaggtcga gaagccatag
    17461 gctctggcgg cttcaatttc cccatgcggc actgaacgga ttgccccggc aaaaatctcg
    17521 gtagtgtaag cgcaggtgtt aagcgtcagc gccagcacgg tacagttcag accactgcga
    17581 aaaaaggcgt taaggaactc ggttccctta acaatctcca gcgtgtacat gccggaatag
    17641 aacaccagca actgaacata cagcggcgta ccgcgaaaaa tataggtaaa taaccagatt
    17701 ggaaactgga tgtatttatt acttgagacg cgaccaatcg ccagaaacag cgccagaact
    17761 ccgcctatca ctaccgacaa aataagcagc cacagggtga tcgccacgcc agtaaagcga
    17821 taaccgtcgg tccacagcag tggtttccag tattcatgta agatttcgat cacaggtcag
    17881 ccctcttcac acccacggag tagcggcgct caaggaacag cagcacacca ttggaaacag
    17941 tggtgaaaac caggtaaatc acgccacaga cgatggcgaa atagaacggt tcccaggtac
    18001 ttttgcctgc cagctgagtg gctttgacca catcttccag gccgagtaac gaaacgagtg
    18061 cggtagattt gaggatcacc tgccagttgt tgccaatgcc tggcagggcg taacgcatca
    18121 tcgccggaaa catgatccgt cgaaacactt gcccacgagt aaaaccgaac gccgtcgccg
    18181 cctctatgtg tcctttcggc actgccataa acgcgccacg aaacgtttcg gtaaaataag
    18241 caccgtagat aaaaccgaga gtgataatac ctgcgaccat cggatcaata tcaatctgcc
    18301 cgacgcccat cgcctccgtc accgtgttta gcgcaatctg caaaccgtag aaaatcagca
    18361 gcatcaacac caagtccggc acgcctcgaa tcagcgtggt gtacccttcg aaaatcagcc
    18421 ccgaaagccg attttgcgag agcttaccgc cagcgccaat taaaccgata attacagcga
    18481 gcactacaga gctgatagcc agctccagcg tgacgagcgc accctgtaaa ataacacctg
    18541 aaaacccata caacatgctg cctgtcctgt cgtgtgtggt gaattaccgc ctcttgtctc
    18601 cctccggtta cccgaaggga gcggactttc tttcaccgat ggcgattaac caccataaac
    18661 atcaaaatcg aagtactttt tcgctaattt ctcgtaagta ccgtcagcgc gcatttcggc
    18721 aaaggctttg ttcagtgcct cgcgcagttc gttatcttct ttacgcaggc ccatgccggt
    18781 ccctacgcca aacagttttt catctttaac agacggacca ccgaatttgt aatctttacc
    18841 gacgggttgt ttgaggaaac cttcgctggc agcgacctca tcctggaacg cggcatcaat
    18901 acgtccggca gtcaggtcag aataaatgtt gtcctgcccc tgataagaaa cgatttcaat
    18961 gccttttggt gcccaatgtt cgttaccgaa cgtctcctgg gtggtgccct gcaatacgcc
    19021 tacccgtttg cctttcagcg actcgactgt cggctgaatg tcggaatttt tcgccaccac
    19081 cagacgagaa tcggcagcgt acagtttgtc ggtgaaggca atttcttgct gacgtttttc
    19141 cgtaatggaa agcgatgaca tgatggcgtc aatcttcttc gcttttaagg acgggattaa
    19201 cgcatccagt gggttttcga caaacgtaca ttgcgtattg atgcgtttgc acaactcctt
    19261 cgccagatcg atatcgaagc caaccagttc gccttgtgaa ttttttgatt caaatggcgc
    19321 ataggtcgga tcggtaccga tgcggatgtt ttgcggaatc gcagcaaacg ccgcagttgc
    19381 gctggagaag gccagaacca gagagagcga tagcaccagt tttttcataa tgtcctcaac
    19441 tgacagtctt tttataggga atatttacag gtttgttaca cttatcgtgc cataaaattg
    19501 accatcaagg caaaatatcc cgccccgtta gacgtattat ttttcattaa aaatgcttaa
    19561 gtatgcattt caatgcacca cgatagtgca acaaacctgg cgtgcaccat gatgatcatt
    19621 ccatcaggta cagtttccct gcgacgtatc agtcaccgta gacattaaag tcgaaatact
    19681 ttttcgccat cttgtcgtag gtaccgtcct gacgcagctc gccaagcgcc ttattaaacg
    19741 ccgccgtcag ttcagcatca tctttacgta gccctacacc ggtaccgtca ccgaagtatt
    19801 ttttgtcttt cactgatgag ccagcaaagg cgaaatcttt accagcaggt tgcttgagga
    19861 aaccttcgct ggccgccact tcatcctgca gtgcggcatc cagacgtcct gcagccagat
    19921 cggaatagac caaatcctgg ttggcatagg ccaccacatc cacacctttg ctacgccagg
    19981 tctcgttagc gtaagcttcc tgggttgatc cctgcagcac acccacatgt ttacctttca
    20041 gtgaatccag cgttggctga atcggcgaac ctttggcggc aatcaaacga gaatctgcgg
    20101 cgtacaactt gtcggagaaa gcaatctcct gctgacgttt atcggtaatg gagagcgacg
    20161 aaataatggc gtcgattttt ttcgctttca gtgaggggat cagcgcgtca aagtcactgg
    20221 caacccaggt acatttcacc tgcatccgtt tgcacatctc gttaccgaga tcgatatcaa
    20281 agccaacaaa gtcacctttg gcatctttcg atgagaacgg cgcataggtg gtatcggttc
    20341 cgatacgtac cgtctccggt agcgccgcat agctggaagc cactgcggag agaccgacta
    20401 acaaagacag agcgagaatc gacttcttca tacataaccc tcaagtgaaa atggcttttt
    20461 tatgttgtgt attgtgttgt gtgtttgcag gctctttcat gcaggtctta tgccatcttt
    20521 acgacaacag taacattcaa cgttaaatat gttaataaga cgttgcatta ttgtcctaaa
    20581 gttgaagata gcaggtatgg cggttgaatc gcagcgtttc gattcatcga aaggaacaaa
    20641 atgtcgagga tttgatcgcg gttgcaaaat tgccctgaaa cagggcaaca atgaggttat
    20701 gcgccctgcc agcgggcaaa gagatcttca ggaagggtta tcgcaaactg gtcaagaaca
    20761 cgattaaccg tctgatttat cacatcatca agggattgtg ggcgatgata aaacgccgga
    20821 acgggaggca taatcaccgc accgatttct gccgcctgag tcattaaacg cagatggcct
    20881 aagtgcagtg gcgtttcacg cacgcagagc accaacgggc gacgctcttt cagcaccaca
    20941 tctgccgcac gggtcagtaa gccatcagta tagctatgga caatgccgga cagggttttg
    21001 attgaacagg gtaaaatcac catccccaac gtctggaaag aaccggaaga aatgctggca
    21061 gcaatatcgc gcgcatcgtg tgtcacatca gctaacgcct gcacttcgcg cagggaaaaa
    21121 tccgtttcga gggataaggt ctggcgcgct gcctggctca ttaccagatg cgtttcgata
    21181 tctgtgacat cgcgcagaac ctgtaataaa cgcacgccat aaatcgcgcc gctggcaccg
    21241 ctgatgccta caatgagtcg tttcataaat agttgccctg tcagacttgc aggcagactt
    21301 tgcaggattt cgacgggagt tgcaagtcag ggtgccagac cggcaccctg agcgaaggca
    21361 tcatccttcg ttatgcattt cgagattttc cacttcgttc tgacgttgca ctgctttggc
    21421 gtcgtcatta cgtaaagtat cgaggaaatc gaggtagccc tgatcaacgt ctttggtgac
    21481 gtagacgccg ttgaataccg agcattcaaa ctgctggata tccgggtttt cagcgcgaac
    21541 ggcttcgatc agatcgttca gatcctggaa aatcaacccg tcagcaccga tgatctggcg
    21601 aatttcatct acttcgcgac cgtgagcgat cagttccgtg gcgctcggca tatcgatacc
    21661 gtaaacgttc gggaagcgaa tttccggtgc cgcagaagcg aggtacactt tcttcgctcc
    21721 ggcttcgcgt gccatctcga taatctgctc agaagtggtg ccacggacaa tggagtcgtc
    21781 gaccagcaag acgtttttat cgcggaactc ggcgcggttg gcgttcagtt tacggcgcac
    21841 ggacttacga cgcagctgct ggcccggcat gatgaaggtg cggccaacat agcggttttt
    21901 aacgaagccc tggcggtacg gtttgcccag aatacgagca atttccagcg cgatatcaca
    21961 cgaggtttcc ggaataggga tcaccacgtc gatatccaga tcttcccatt cgcgggcaat
    22021 tttctcgccc agtttggtgc ccatattcac gcgcgcgctg taaacggaaa ttttgtcgat
    22081 gaacgagtcc gggcgggcaa agtatacata ctcaaacagg cacggattgc tgaccggatt
    22141 gtcagcacat tgacgggtaa acaactgccc ttcttcagtg atgtaaatcg cttcgcccgg
    22201 cgcgacgtca cgcaggaaat caaagcccag cgtatcgagc gcaacgcttt cggaagcgac
    22261 catatattct gtacggttct cgtcaatatc acgttttccc agtaccaacg gacgaatacc
    22321 gtttggatcg cggaaagcaa ccataccgtg gccgatgatc atcgccacac aggcatacgc
    22381 gccgcggatt aagcggtttg tagcagcgat ggcggcgaaa atgttgtcgg cttccagcgg
    22441 atagtggcgg aagttgtcca gttcgctggc aaagatatta agcagaattt ccgagtcgga
    22501 agtggtgttg atgtggcggc gtttttcttc aaacagtttt ttacgcaact cgtgagcgtt
    22561 ggtcagattg ccgttgtggg caagcgtaat gccatacggg gagttaacgt aaaacggctg
    22621 cgcttcagag gcgctggagc tgccagccgt agggtaacgc acatgaccaa tgcccatatt
    22681 gccctgcaaa cgctgcatat ggcgagcttc aaatacatcg ctcaccagcc cgttcgcttt
    22741 acgcaaacgg aagcagttat tggcatctat ggtgatgatg ccggcggcat cctgaccgcg
    22801 atgctgaagc accgttaagg catcataaat cgactggtta accggcataa caccggcgat
    22861 accgacaata ccgcacatac gtctttttcc tcgttaagcc acatctcaga gcacttacgc
    22921 tctgggcaag aaacttgacg agctttgcag ataatcaaaa aaccatctga tgataaaact
    22981 gaactgcggg atcagctgtg atttgctcca gtcttcgctt ttcgacaccc cggtaaagga
    23041 gtcgagaaag aagagaatgg cggcaacaat caacacaccg cgcaacgcac cgaaacagac
    23101 gcccagcacc cgatcggtgc ctgacaaccc cgttttctct accaactggc ctatcacgaa
    23161 gttcacgata gcaccaacga tcagggtagc gataaacagt accgcgatgg caatcccatt
    23221 tcgaaccagt tcgtcttcaa agcccgtaaa ccagactgac aggtaagtgt agtaatgact
    23281 ggcaacaaag aaagcacaac cccatgtcac cagcgataac gcttcacgaa caaagccgcg
    23341 gatcaggcta accagagagg aaaaagcaat caccgcgatt atggcgtaat caatccagac
    23401 catatgtgtc ccacgatttt acgccctgtc atcctgttcg gggcgcattc taacagaaaa
    23461 agaaaacgtt tgcgtaggga tttccttccc gcgcatcaat aaaaatggcg ctgaaaaaac
    23521 attcaacgcc atcgactttt tatgcctttg cggcatcggg caatgcgtgt cggatgcggc
    23581 gtgaacacct tatccgacct acggttctac ccctgtgtag gcctgataag acgcgccagc
    23641 gtcgcatcag gcaagaccgt attaattcgg cgtatagccc attaccacgc cacttaagcc
    23701 agaaagttgc ttcaactcac ccagcgatcc tttcagctta tctttcgagg catcaggccc
    23761 aaccagaata cgggtaattt taccctgcac tggcgtggat ggcgacgtat aaacccgata
    23821 accggcaccg cgcagcttac cgacaatctc attcacttta tcggcatttt tcagcgcgcc
    23881 cagttgcaca acataggctt tacccgttgg cgctgctttt tcttccacga ccggcttcga
    23941 cgcaggtggt gcttcaacct tcggtttagg cgactccacc ggtttcggct tcggtggggc
    24001 gaccggtgca ggttccggtt caaactcggt gttattgact gcaatagtgg ccggatcgag
    24061 cgacggtgct gcggcatcac ctgcccgcac ctcttccgct gcgccttccg gcggctgcgt
    24121 cggcagcgcc tgggtggcgg ctggcatcat atcaggctca tcgcgatcgc ccgctttcgg
    24181 caccagcggg atagccgcga actcatcctg ataatgtttt ttctgcccgt ccagcagccc
    24241 tggaagtaca atcaccccca gcgccaccag cacgatcgtg cccactaacc gattctgaaa
    24301 cttacttgcc accgcttctc ctcgcgtcaa tcacttccat gacatgtgcg accgtgtgga
    24361 acgaaccaca caccagcacg gtatcttccg ctttagcgtc cgccattgcg gcatcccatg
    24421 cctgcgccac gctatcaaat gatttgccgt tacccaaatg ctcaagcagt tgttctgccg
    24481 tggcaccgcg cggcccttcc agtggcgcac aataccagtc atcaaccacg cttttcaacc
    24541 aggccagagt tccggcaata tctttatcat gtagcatacc gataaccgcc agcacgcgcc
    24601 cgtttttcgg tagcgctttc atacgcccgg tgagatattc cgccgcatgt ggattatgcg
    24661 cgacatcaaa aataacgcgt ggcgactcgc tcacaatctg gaaacgtccc ggcaaaattg
    24721 cgctggcaat cccgtcgcga atggcatttt cactgacctc cagcccgctg gcacgcagtg
    24781 ccgccagcgc cgttgcggca ttcggttgcg ggacaagcgg caacggcaga ttttccagcg
    24841 tgccgtgagc atcgctaaac gcccagtcat gatcggtgac ggaatagttc cactcaacgc
    24901 cccgacgttg taacagtgca cttttttcct gcgccacatc agcaatggta gaaggcattt
    24961 ccggctcacc gacaattgcc ggtttttcgc tgcggaagat gcctgctttc tcgcgaccaa
    25021 tgctttcgcg atctggcccc agccagtcgg tatgatccag cgcaatgctg gttactaccg
    25081 caacatcggc atcgacaata ttggttgcgt ccagacgacc gcccagccct acttccagaa
    25141 tcaccacgtc aagttgtgcc tgtttgaaca gccacaacgc cgacagcgta ccgtactcga
    25201 aataggtcag ggaaatatca ccgcgtgccg attcaatctc cgcaaaagag gcggtgtggg
    25261 ccgattccgg caattcctgg ccctgcacac gtacgcgctc ggtataacgc accagatgcg
    25321 gcgaagtgta gacgcccact ttgtaccctg ccgccatcag aatcgactcc agcgtacggc
    25381 aagtggtgcc tttgccattc gtacccgcaa cggtaaacac aaatggcgca ggtttcagga
    25441 cgccaagacg cgccgcgacc tggctcacgc gctcaaggcc gagatcgata gttttactgt
    25501 gcaggttttc cagataagaa agccacgaag ccagaggcga cgcggcttga ggagtgcgtt
    25561 tgataatcat ggtatccgct gattcgttac ggtgagaata gcaaaagggc agagccagtg
    25621 gccctgccct tatcagttat caggcctcag gttcctgatc tggtaccggt ggcaccacta
    25681 cgccttcacg cggcgcttca ggattcggcg ctggcagatt catcaacttc gccagaatgc
    25741 tcgccagttt caggcgcatt tccggacgac ggacgatcat gtcgatcgcg cctttctcga
    25801 tcaggaattc actgcgctgg aatcctggcg gcagtttttc gcgaacggtc tgttcgataa
    25861 cacgcggacc ggcaaagccg attaacgctt tcggttcagc gatgttgaga tcgcccagca
    25921 tggcgaaact tgcagaaaca ccgcccatgg tcgggtcggt cagcacggag atgtacggca
    25981 agccgcgctc ctgcattttt gccagcgccg cagaggtttt cgccatctgc atcagcgaca
    26041 tcagtgcttc ctgcatacgt gcgccaccag aggcggagaa gcagatcagt gggcagttat
    26101 cttccagcgc ctgctcaacg gcacgcacga aacgtgcacc taccacagac cccatggaac
    26161 cgcccataaa ggcgaactcg aatgccgcag cgacaactgg cattccatac agggtgcctt
    26221 tcatcaccac cagcgcatct ttttcgccgg tttctttctg cgcagatgcc agacggtctt
    26281 tatacttctt ggagtcacga aacttcagca catctttcgg ctcaagctcg ctacccagct
    26341 ccacaaggct tccttcatct aacaggctat gcaggcgatt acgcgctgtc atacgcatgt
    26401 gatggtcaca cttcggacag acctcaagat tacgttccag ctcagcgcgg tataaaacct
    26461 gaccgcagct atcacactta gtccacaccc cttcaggaat gctcgccttg cgggtgggag
    26521 taatgttgct tttaattcgt tcaatccagc tcattaggga cctttctgtc tgaacctggt
    26581 tcgatgccag ttttatcttt ggggacgcat aatgccattt ttgcccccaa cagaccatga
    26641 atgttgcaca ttaaaacata acagcccgaa actttggata aaaaagtggt cgaaccgcgg
    26701 agttactttt tattttgcgg cgcgtgccgc agcgcgtttg tggcgaataa attcgaaaac
    26761 acctggcaat attgaaacaa aaataatgcc aacaatcatc agtttaaggt tgctctgaat
    26821 gaaggggagc gttccgaaga aataacccgc gtaggtaaaa atcagtaccc atagcaacgc
    26881 gccgatgacg ttaaaagcgg cgaaatgacg atacgacatg tggcccattc ccgcaacgaa
    26941 cggcgcgaac gttctgacga tcggcacaaa acgggcgaga ataatcgttt tgccgccatg
    27001 tttctcataa aactgatgag tcttatcaag ataactgcga cgaaagattt tcgaatccgg
    27061 attactgaac agtttgtcac caaataaccg tccaatggtg tagttcaccg cgtcacccac
    27121 aatcgcggcg atcagcatca gcaccaccat catatggacg ttgagatcgt tggtttccag
    27181 tgatgccaac gcaccggcaa caaacagcaa tgaatcaccc ggtaaaaacg gcgttaccac
    27241 cagaccggtt tcacagaaca aaattaagaa caaaatggca taaacccaga cgccatactc
    27301 cgcgaccagt tctgccagat gcacgtcaat gtgcaggata aaatcgatga ggaaataaat
    27361 caggtccata ttagcctatg ccttgtactc gtcatctttc aggctgtaac tacgttggct
    27421 acagcctgaa atgttccgag cattattctt gttagtccgc cagaaatagc gggcccattg
    27481 gcggttttgg aagatcatac cggtcagggt aatccaccgc gaccagatac agcccttccg
    27541 cttttgccgt tgctgccgcc agcgttctgt cctttgccgc cagcaactct gctatccagc
    27601 tctccggctg gttgtgggca ccgacttcca tcaggctgcc gacaatattc ctgaccatat
    27661 gatgtacaaa ggcattcgct ttgatatcta ccaccacata aggaccgtga cgcgtgacat
    27721 taatatgcat aacgttgcgc cacggggttc gggactggca ctgcaccgca cggaacgagg
    27781 tgaaatcatt ctcgcccagc aagcattgcg cagcccgatg catccgttca gcgtccagcg
    27841 gttcgtaaaa atgggttacc cctttactca gtaccgccgg gcgcagccga tgattgtaga
    27901 tgatgtagcg ataacggcga gccgtggcgc taaatcgggc atgaaaatca tcaggtacag
    27961 ctttaaccca acgcacggcg atgtcaccag gtaaattcgc atttacgccc aacgtccacg
    28021 cagcgtcttt gcgctgtgcg gtcgtttcga aatgcacaac ctgcccggta ccgtgtacac
    28081 cggcgtcagt acgcccggcg cagaagacgg tgatgggttc gttcgccacc tgggagagcg
    28141 ccttttccag cttctcctgc acactgcgga cttcattctg ccgttgccag ccgtaatacc
    28201 tactgccgtc gtactcaatg cccagcgcaa ttttataaac tggcggttgt tgctggtcgg
    28261 acattagtac agatactcct gcaccagttt ctcggcgatt tttactgcca tcagcgcgcc
    28321 gccaaagcga acgttatcgg ccaccgacca gaactgaact tgctccggca taccgtagtc
    28381 attacgtacg cagccaacag aaagatgcgg cgtaccagaa gcatctccta cctgagtcgg
    28441 gaattcgttc tcttcagaga gcacaatatc ttcgccctga gcaaacgcat cacgcgcttc
    28501 ttctgccgcc agcggacgca gtgcttcaaa gttgaccatc tgggcatgac cgtagaatac
    28561 cggtgcctgg acgacgcttg ccgaaatcat cagcccttcg tcctgcagga ttttgcgtac
    28621 ttcgtcaacg atacgacgtt cttcacgcac gctaccttcg ctatccggca gtaacggcag
    28681 catgttgaac gccagctgac gcccgaagaa atcttcttcg tcaatcggaa tgccattgag
    28741 taatttagca ctctgccccg ctaacgcatc gaccgctttt ttgccctggg cggaggctga
    28801 aatcaggctg gtaacgctga tacgtgataa accgccctga tcaattagcg gtttcagtgc
    28861 cgccagcagc tggctggtca gactgtccgg tacggcgatg acattccggt tgcggtaatc
    28921 ggtcagtaca aacgggttta cttccggcac taccagcggt acgtcaggtt cgagagcaaa
    28981 caatccgctg ctgtcgatca ccagacaacc tgagttggtc gcttcttcaa cccaggcagc
    29041 ggtagcttct ttgcctgcga caaaaaatgc cagctgcgcc tgcgtccagt caaattcagc
    29101 ggcatcctgc acggtgattg tcttaccacc aaagcgcagt tgttcgcctg cgctttcgtt
    29161 acgtgccagt gcataaattt ccccaaccgg gaactgacgt tcagccagcg tttcaagcag
    29221 ggcttcgccc acagcgccag ttgcgcccag gacggcaatg ttccagcctt cagacatggt
    29281 ggtttactcc agaaatagca aagctccctg ccaatgttac agcagagagc atgaagaaga
    29341 gattaacgtg ccggatgatg aacggcgtta aaacccagtt tacacagcaa tgatgccgca
    29401 ctggcgtcat cacaaattac atacagagat gaccattcac ggcgctcaag atagtttttg
    29461 cgcagtttat cgaactcacc cggtatcccg gcgactttac gcagtggcgc atcatcgcgg
    29521 cgcacatcat acaccaaatg caccagcctt ttcagcgtcg gttgatcgag cggaccatgc
    29581 agcgtaatgc gaccaaactc tggcgcgggc agtaatgtat ccagcgcaac gtgttgttca
    29641 tgcccaatga atttgctata ggcttcaaac acttgcgtag taccgcgtgc tttaccttcc
    29701 agggtatagc ctgcaatatg cggcgtgccg atatccactt ttgtcagcaa ttccacatta
    29761 agttccggct cgccttccca gacatccagc accacgctta acttctggcc ttcattcagg
    29821 caggtcagca gcgcggtatt atcgacgact gcgccacggc aggcgttaat cagaatcgct
    29881 ccgggcttca ggctacggat cagtttttca tccgccagat gtcgcgtttt gtacggacca
    29941 tctttaaaga gtggcgtatg gaaagtcaga atatccgcat gctggactaa ttcatccagc
    30001 gagcggaaat cgccctcatc cccacggtcg gcgcgaggcg gatcgcaaag caaggttgta
    30061 atccctaacg cttccagtcg tgcctgtaaa cgccgaccaa cgttaccaac gcccacgatc
    30121 cccacggtac gctcgtgcag tgaaaatcca tcgcgttcag caagcatcag cagtgaggag
    30181 aaaacatatt ccactactgc aatcgcatta cagccaggtg cagcggaaaa accaattccc
    30241 gcctgcttta accatgtgtc atcaacatgg tctgtccccg cggtggcagt gccaacaaat
    30301 ttaatgggtt ttcctgccag caaagattca ttcactttcg tgaccgaacg caccatcagc
    30361 gcatccgcgt ctgccagttg agccacgggg atgggacgcc cgggaaccgc ggtcacctca
    30421 cccaaacggc taaataagtc gcgggcataa ggcatatttt catcaacaag gatcgttaat
    30481 aatttttcag tacgcgacaa tctggccccc cctttatcag caaacaccct tttaacccca
    30541 ccgtacaaca tgaaaaccat ttcataatca tacacataca aacgcaacca tttagacggg
    30601 tgatactgga aagtatgttt cacagtatca cccttaaagc tatttcagac cagacaaaat
    30661 cactctcccg tggacaccga gtggacactt acgccacctt tcagcggatt aagagccacc
    30721 gcatcctgca aataatccgg cgcaaaatgt gcatacgcca ttgtttgctg gatagtcgcg
    30781 tgacccagga tcctctgtag cgcaataatg tttcccccgt tcatcacaaa atgactggcg
    30841 aacgtatgtc gcaacacatg cgccgcctgt ccttttggca aatcgggttt aacccttttc
    30901 agcaccaggc aaaattcccg gtacttcaca tcaaataatc cccccgtctc gctggttttg
    30961 atcgcctcac aaaccgcctg cgagattggc accgttctct tacgaccgtt tttggtctca
    31021 agaaacgtca cacgattatg aactatctgt tcaccacgaa gcttactggc ttcgccccag
    31081 cgtgcccccg tacttaaaca caaaagcgca acacgccagt aatcgccctc tagtgtatcg
    31141 agcaataacg ccacttcttt ctgggacaag aaagccattt ctcgcgggga cacataaagc
    31201 acagagattc ccctgaccgg gtgctctgca tcccatagtc ctattttttt cagagcggta
    31261 aacattccgg ataaccggtt catatacctg ttagccgatg acggcttcag tccgtcagcg
    31321 atcctgcgag aacgccaggc aatgattttc agcttatcca gatccaccgc ctgcatatca
    31381 gcaccaagtt cattgattat gttgcgtaat tgttttcggt cttcctccgc tttacgcctg
    31441 tgctggccgt gatacaccca ccacaactcc agcaaatcgt tcaacgtccg gcgatcacgg
    31501 tagccctgta tatattcccg cttctcagca ttcgccatga tgtagcgctc agtggccacc
    31561 gctaccgatt ttttgttaaa taccttacgc acgcgttttc ccttgcgtcc gttcggcctg
    31621 atatccagca aataacgacc atcttcgagc ttcttaatcg acatggcgaa gccctccaat
    31681 gaaccgtttt acaatttcac cagcttcttc ccagcaatag tcagaccagg caaaaagcag
    31741 gtccaaccag ttttctggcc tcagcggggt aattttcggg ttgtttcggt tgagtcctga
    31801 ttgccctcca aattgcagga gccatcaaga gagagagccg gggctatctg tccggcctcc
    31861 ggcattgttt catcagttga taaccagtag gcgtacttct taaatttagg gtgttttgta
    31921 accttaagta aggccccctc tgttacttgc ttccccctca cctcatagtt agttactgtc
    31981 ccataaggca accccacgca atccgcaaac tcctggcggg tcataccttc agcttcacgt
    32041 atcaggcgaa atttttcacc catgcttgac aaagatgcca tatcgggcat atcctccatt
    32101 gcaaacgtgc cacatcgggc acacacataa gaagccaaaa taagccactt tgggccattt
    32161 tgagccattc gaacgaatca aggagattac cacaatgaga gaatcagagc ttgagggctt
    32221 cattcaggta gcgccatatc cacttgaggc ggtgccatat caactattcg ccaagatgat
    32281 tggccgcaaa gaatccaccg tcagaaccat gattgacgcc gcaaagctac caacaattga
    32341 ctttgtgaaa ccgggttcag tgaaaactcg tgcatcagaa aactgggtat atctgccagc
    32401 atttaacgca ggtatgcgaa aagcattttt taatcaaccc aaagaacaac gcgatgcatg
    32461 gctgttatgg ctgggtctgt aacgatatat ccaggaattt ttcaaaacca atatatctac
    32521 tctgagagag ttgtttgggc ataaaaaagc acaggataac taagctgata acccactaca
    32581 gacacaaaag gaaacagcca tgactacaca aaaagcaaaa accataatta aacgatacaa
    32641 cgtacgtagt ggtgtggctg gcgttgtctt ttccgatgtg acacagcgac gcagcttccg
    32701 cactggcaca gaacgccacg cagcccgttt ctcaaccagt gcatcacgca gcaacacgca
    32761 ctacagcctg caggaaacgc acgccacgcc tgacgggcat ctggttaaac aaaccggtaa
    32821 acacacctgg ctgattaatg atgccggaat tgtcatacac ag
//
LOCUS       DAZGQL010000059        32283 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.059, whole genome shotgun sequence.
ACCESSION   DAZGQL010000059 DAZGQL010000000
VERSION     DAZGQL010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32283
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.059"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(6..1358)
                     /gene="creD"
                     /locus_tag="ACHKIR_002999"
     CDS             complement(6..1358)
                     /gene="creD"
                     /locus_tag="ACHKIR_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HFO9990703.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNITLGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLKLNMALNLSGTGDLSVVPGGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAHWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFIAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHTGFTVAWIIASLIGAIMNGIYLQAVLKGWCNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(1416..2840)
                     /gene="creC"
                     /locus_tag="ACHKIR_003000"
     CDS             complement(1416..2840)
                     /gene="creC"
                     /locus_tag="ACHKIR_003000"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HFO9990704.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDTTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPTDPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVTPTEVNVAAEPALLEQALGNLLDNAIDFTPESGCITLS
                     AEVEQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(2840..3529)
                     /gene="creB"
                     /locus_tag="ACHKIR_003001"
     CDS             complement(2840..3529)
                     /gene="creB"
                     /locus_tag="ACHKIR_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HFO9990705.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLTLHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(3542..4015)
                     /gene="creA"
                     /locus_tag="ACHKIR_003002"
     CDS             complement(3542..4015)
                     /gene="creA"
                     /locus_tag="ACHKIR_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HFO9990706.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            4226..5095
                     /gene="robA"
                     /locus_tag="ACHKIR_003003"
     CDS             4226..5095
                     /gene="robA"
                     /locus_tag="ACHKIR_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HFO9990707.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(5092..5739)
                     /gene="gpmB"
                     /locus_tag="ACHKIR_003004"
     CDS             complement(5092..5739)
                     /gene="gpmB"
                     /locus_tag="ACHKIR_003004"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HFO9990708.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLETRNIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            5791..6306
                     /gene="yjjX"
                     /locus_tag="ACHKIR_003005"
     CDS             5791..6306
                     /gene="yjjX"
                     /locus_tag="ACHKIR_003005"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HFO9990709.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLHPEADFWVAIEAGIDGDSTFSWVVIENASQRGEARSA
                     TLPLPAVILEKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQ"
     gene            complement(6300..6626)
                     /gene="trpR"
                     /locus_tag="ACHKIR_003006"
     CDS             complement(6300..6626)
                     /gene="trpR"
                     /locus_tag="ACHKIR_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HFO9990710.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKDH"
     gene            complement(6716..8653)
                     /gene="sltY"
                     /locus_tag="ACHKIR_003007"
     CDS             complement(6716..8653)
                     /gene="sltY"
                     /locus_tag="ACHKIR_003007"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HFO9990711.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWELSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            8989..10656
                     /gene="ettA"
                     /locus_tag="ACHKIR_003008"
     CDS             8989..10656
                     /gene="ettA"
                     /locus_tag="ACHKIR_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HFO9990712.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDSLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(10712..10996)
                     /gene="nadS"
                     /locus_tag="ACHKIR_003009"
     CDS             complement(10712..10996)
                     /gene="nadS"
                     /locus_tag="ACHKIR_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="HFO9990713.1"
                     /translation="MSFFDELKTSLEEAVEIKQGLKEPARVTRYEIADVKAIREQLNV
                     SQSEMAKALGTSVDTIKSWESKRRNPTGLAAKVLATIKENPDFFRELASH"
     gene            complement(10998..11330)
                     /locus_tag="ACHKIR_003010"
     CDS             complement(10998..11330)
                     /locus_tag="ACHKIR_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO9990714.1"
                     /translation="MGKAIIFIETPMFTRQIKQIATDDELKELQKELIGTPDKGDLIQ
                     QTGGLRKVRMAAGSQGKSGSVRIIYFLATEEIIYLIMAYPKNAKDSLTDTEKAQLKKL
                     TKLLKGEI"
     gene            complement(11422..12654)
                     /gene="nadR"
                     /locus_tag="ACHKIR_003011"
     CDS             complement(11422..12654)
                     /gene="nadR"
                     /locus_tag="ACHKIR_003011"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0050262 - ribosylnicotinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HFO9990715.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(12675..14057)
                     /gene="radA"
                     /locus_tag="ACHKIR_003012"
     CDS             complement(12675..14057)
                     /gene="radA"
                     /locus_tag="ACHKIR_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFO9990716.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(14106..15074)
                     /gene="serB"
                     /locus_tag="ACHKIR_003013"
     CDS             complement(14106..15074)
                     /gene="serB"
                     /locus_tag="ACHKIR_003013"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HFO9990717.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGERVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKTEVTIRHADLMGVFCILSGSLNQK"
     gene            15180..15824
                     /locus_tag="ACHKIR_003014"
     CDS             15180..15824
                     /locus_tag="ACHKIR_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HFO9990718.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVDVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            15852..16868
                     /gene="lplA"
                     /locus_tag="ACHKIR_003015"
     CDS             15852..16868
                     /gene="lplA"
                     /locus_tag="ACHKIR_003015"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HFO9990719.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTAEGDRKVSGSAYRETKDRGFHHGTLLLNSDLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITEAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFTWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPEQEKELRELSAWMA
                     GAVR"
     gene            16900..17163
                     /locus_tag="ACHKIR_003016"
     CDS             16900..17163
                     /locus_tag="ACHKIR_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO9990720.1"
                     /translation="MIDLENQEREIINLMFSQGISWLTAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAGIYGCPPELLICPSWMTAEHK"
     gene            complement(17324..18043)
                     /gene="deoD"
                     /locus_tag="ACHKIR_003017"
     CDS             complement(17324..18043)
                     /gene="deoD"
                     /locus_tag="ACHKIR_003017"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFO9990721.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(18100..19323)
                     /gene="deoB"
                     /locus_tag="ACHKIR_003018"
     CDS             complement(18100..19323)
                     /gene="deoB"
                     /locus_tag="ACHKIR_003018"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFO9990722.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     HGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(19375..20697)
                     /gene="deoA"
                     /locus_tag="ACHKIR_003019"
     CDS             complement(19375..20697)
                     /gene="deoA"
                     /locus_tag="ACHKIR_003019"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009032 - thymidine phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HFO9990723.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKNDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            complement(20775..21554)
                     /gene="deoC"
                     /locus_tag="ACHKIR_003020"
     CDS             complement(20775..21554)
                     /gene="deoC"
                     /locus_tag="ACHKIR_003020"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFO9990724.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            21812..23362
                     /locus_tag="ACHKIR_003021"
     CDS             21812..23362
                     /locus_tag="ACHKIR_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HFO9990725.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDEIDIRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAELKQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIAN
                     GPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEG
                     IAARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            23334..24197
                     /locus_tag="ACHKIR_003022"
     CDS             23334..24197
                     /locus_tag="ACHKIR_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HFO9990726.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHIRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSICLLAERGKLAELRLLVIPGQ
                     VDYLQHIEELAALIKGLGDVPVRLNAFHAHGVYGEAQSWPSATPEDVEQLADALWERG
                     VSRLIFPALYL"
     gene            complement(24415..25194)
                     /locus_tag="ACHKIR_003023"
     CDS             complement(24415..25194)
                     /locus_tag="ACHKIR_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026291.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO9990727.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLKKHSDVSLDQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPRTGVVHGFS
                     GSLQQAERFVLLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFDVLCELRPEPEDEIAEVLLNNTYTLFNVP"
     gene            complement(25191..26264)
                     /locus_tag="ACHKIR_003024"
     CDS             complement(25191..26264)
                     /locus_tag="ACHKIR_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HFO9990728.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAYICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGQLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVIDAPQANDPTFNNEDLA"
     gene            complement(26386..26547)
                     /locus_tag="ACHKIR_003025"
     CDS             complement(26386..26547)
                     /locus_tag="ACHKIR_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HFO9990729.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(26674..27279)
                     /gene="osmY"
                     /locus_tag="ACHKIR_003026"
     CDS             complement(26674..27279)
                     /gene="osmY"
                     /locus_tag="ACHKIR_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HFO9990730.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(27672..29261)
                     /gene="prfC"
                     /locus_tag="ACHKIR_003027"
     CDS             complement(27672..29261)
                     /gene="prfC"
                     /locus_tag="ACHKIR_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFO9990731.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQSAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(29352..30029)
                     /gene="yjjG"
                     /locus_tag="ACHKIR_003028"
     CDS             complement(29352..30029)
                     /gene="yjjG"
                     /locus_tag="ACHKIR_003028"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HFO9990732.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLNVEPGKLNEAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(30044..30490)
                     /gene="rimI"
                     /locus_tag="ACHKIR_003029"
     CDS             complement(30044..30490)
                     /gene="rimI"
                     /locus_tag="ACHKIR_003029"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006474 - N-terminal protein amino acid
                     acetylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFO9990733.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(30459..30872)
                     /gene="holD"
                     /locus_tag="ACHKIR_003030"
     CDS             complement(30459..30872)
                     /gene="holD"
                     /locus_tag="ACHKIR_003030"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HFO9990734.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPIVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            30975..32006
                     /gene="rsmC"
                     /locus_tag="ACHKIR_003031"
     CDS             30975..32006
                     /gene="rsmC"
                     /locus_tag="ACHKIR_003031"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HFO9990735.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATADDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLSDYAPLNKMDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
BASE COUNT         7571 a         8880 c         8237 g         7595 t
ORIGIN      
        1 caaccttatt tccagatacg taattcatcg tccgttgtaa cttctttact ggctttcatt
       61 ttcggcagtg aaaacgcata ccagtcgata ttacgggtca caaacatcat gccggccagc
      121 gccaccacca gcacactggt tcccaacaac agcgcgctat cggcagagtt gagcagtccc
      181 cacatcacac catccagcaa caacagcgcg agggtaaaca acatgctgtt gcaccaacct
      241 ttcaataccg cctgcaaata aataccgttc attatcgccc caatcagact ggcgattatc
      301 catgccacgg taaaaccggt atgttcagaa agcgccagca agagcaaata aaacatcacc
      361 aatgaaagcc ccaccagcaa atattgcatt gggtgtaaac gttgcgcggt gagcgtttca
      421 aaaacaaaga acgctataaa agtcagtgca atcagcagaa tggcgtactt agtcgcccgg
      481 tcagttaatt ggtattgatc ggctggcgtc gttactgcga cgctaaacgc cgggaagttt
      541 tcccagccgg tatcattgcc tgaagcaaaa cgctcaccga gattattagc aaaccagctg
      601 ctttgccagt gcgcctgaaa acctgactcg ctaacttccc gtttggctgg tagaaaatca
      661 cctaaaaaac tgggatgcgg ccagttgctg gttaaggtca tttcgctatt acgcccgcca
      721 ggcaccacag aaagatcgcc ggtaccgctt aaattcaggg ccatattcag cttcaggttc
      781 tgcttccgcc agtccccttc aggtaaaggg atatgcacgc cctgcccgcc ttgctctaag
      841 ccggtgccgg gttcaatggt cagcgccgtt ccgttaactt caggtgcttt caccacacca
      901 ataccacgcg catccccgac gctaatcaca ataaatggct tgcctaaggt gatatttggc
      961 gcgttgagtt cgctaagacg cgaaacatcg aaatcggctt ttagcgttaa atcactgtgc
     1021 cagacctgac cggtataaat ccctatcttg cgttcttcca cgttctgatt gccatcaacc
     1081 atcaatgact caggtaacca aaaatggata aaacttcgtt tccgctccac ggttttatcc
     1141 tcttcctgca ccgtataaag ctcggtcaca ggaatagcga tgagcggccc aacgagtttt
     1201 tgcggcccgc tggtactttg gcgaatcgca tcttccacat cgctacggta atcagcacgt
     1261 tcgacaatca cctgccgaat cagcattatc ggaatcaaca acagcaatac tgcaccaaac
     1321 agggtggtca ttttccagaa caggggggat ttcaacatag tcttctcctt tgcaatggca
     1381 gcaggatacg aaggctatgt gggaagaatt tgaagctatg tgaagtgacg gtgaagtcga
     1441 agcgaagcca gcacgccacc ttcctgcaca ttgcgcagcg tgacttcgcc gttaaacaaa
     1501 cgggcgacct cactgacgaa cgccaacccc agaccgctgc ttttttgccc atttgcacga
     1561 ggcaaagagt aaaagcgttc aaaaatacgt gaaagcgcgt agtcaggaat accactaccg
     1621 gtatccagca ccttaagcgt gacgtgttcc tgctccactt cggcgcttag cgtgatgcaa
     1681 ccgctctcgg gggtaaaatc gatggcgtta tccagtaaat tccccagagc ctgctccagt
     1741 aacgccggtt cagcagcaac gttaacctcg gtgggcgtaa catgtagagt gatgtttttt
     1801 tctgccaact gcacggtgcg cgcttcgctg acgcggcgga ataatgccgc cacatcaaca
     1861 gcagttagaa cgacttcctg acgattctcc agtcttgcct ggcgtagtaa cgtttctacc
     1921 aatgcctgca tacgcgcatt ttgcgtcaga atgttgtcag taaaacgagc caccacttcc
     1981 ggcggcggac cttcgcgtaa aatttccgcc gctccacgaa tcgccgccag tgggcttttt
     2041 agctcatgag ttaatgcgta aacatactgc tcaatatagt ttttcccttc cagcttcacg
     2101 cgcatacttt ccagcgcctg cgcgagttta cgcagctcgc tactaccgag atcggggaga
     2161 ggaacgggct tattgtcagt gacggaatca gcatagcgag tgagccgggc aatggagcgg
     2221 ttgatccacc aaaccatgcc tgcgccaatc accagtgcaa tccccaacaa aatggcgctg
     2281 gcccataaaa ttcgccgctc gctgcgttta atgaccggag ccatcgccgc gttcggtttg
     2341 cctacgctca aaacgccaat aagccgcgag ccgtccataa tcggcgcggc aacatacatc
     2401 accgaacttt cgggatcggt aggattttgc aacgtgctgc gcgcaccata ctgaccacgc
     2461 aacgttagcc agacgtcatt ccagcgcgaa taatcctgtc caacggcttt atttgccgaa
     2521 tcgaacaata ctttgccctg cgcatcggtc atatagacat gatattcatt gcgcactttg
     2581 ttaatgccac cgatattggc gcgaaacggg cgatgctgta gctgattaaa cgcctgcgcc
     2641 agttgcccat gcgttgtgtc cccagagagc aaatcgggac gcgccagctc cgccagcaac
     2701 gttgcggtgt cgatcaacgt cccctccgtt gctcttcgca cgcccggttt aacttctttg
     2761 acaaaaatgg ccagtacgaa ccaagctgcc accgccacca gtaaaaaata gcccagcaac
     2821 aaccgcatgc cgatacgcat tacaggcccc tcaggctata tcccatgccg cgatgagtat
     2881 taatcggtga aagatcgggg ttgatggcgc gcagcttggc acgcagcgtt ttaatgtggg
     2941 tatcgacggt gcgatcgtag gtgtcctgcg catcttccca cacgctatcc atcagttgct
     3001 ggcgggacca gacgcggcct ggcgacttga gtaacgtctt cagcaataaa aactcatacc
     3061 gcgtcagcgt taatggcgtg tcaaaccagc tgatctgcgc cgcgggttca ttcaattcaa
     3121 aatgaccaat acggatgacg ggagacggcg tcgagaactt cttcacccga cgcagtaagg
     3181 tgcgcaccct ggcgcacact tcgcggggtg aaaacggttt agccacatag tcgtcagcgc
     3241 caatttccag cccaagcagg cgatcgacct cttcacttcg ggcggtcagg aacagtacag
     3301 gtaacgccgg atggagcgtc agtaactggc ggcacaattc aaagccactg atatcaggca
     3361 aaccaacgtc gagaatcatg acatcgggaa cctgctggcg agctttatcc agcaccggca
     3421 agcctcgctc aaagacctcg acggcaaaac cttcctgctg caacatgtag accagcgtgt
     3481 cggctatccc ttgctcatct tccactaacc agaccgtttc ccgttgcata aatcgcctct
     3541 gttattgccg ccacggcatg acaggaaccg cgctaatcgc gtttttcggc gaaccttcta
     3601 caactttgtc ggagtaagcc agataagcga gcgcgttgcg tttagcgtca taaaagcgca
     3661 cgacctgtaa cgacttaaag accagggacg tgcgtttttt gaatactacc tcgccctgag
     3721 ctttgccgtt tttaatacga tccgacagtt caatcggccc gacttgctga caagaaatgg
     3781 ccgcatcgga ggtatcttct gccaggccca atcccccttt aataccaccg gttttcgccc
     3841 gactcacata acaggtgaca tttttcacat cgggatcgtc aaaggcttcc acaacaattt
     3901 tgtgatccgg gccgatcatt ttaaatacgg tgtcgaccga accaatctct tctgcatgag
     3961 ccaatggccc cagcattatc aggcttaaag acaggatcaa atgcttgtat ttcatattgt
     4021 taccattttt atctattaca cgagcaatta gttcgtcacg gtaaataaca tattgattaa
     4081 gaacaaaatc tcaatacttt tatttccgtc aagccctaaa acatactcta ctaagaaaaa
     4141 aacactgaat gctaaaacag caaaaaatgc tattatccaa ttacctgatg tcaggtgctc
     4201 gttgttgaaa ggatgaggat attttatgga tcaggccggc attattcgcg accttttaat
     4261 ctggctggaa ggtcatctgg atcagcccct gtcgctcgac aatgtagcgg cgaaagcagg
     4321 ttattccaag tggcacttac agagaatgtt taaagatgtc actggccatg ctattggcgc
     4381 gtatattcgt gctcgtcgtt tgtcgaaatc ggcggtcgca ctacgcctga ctgcgcgtcc
     4441 gattctggac atcgcgctgc aataccgctt cgactctcaa cagacattta cccgcgcatt
     4501 caagaagcag tttgcccaga ctcctgcact ttaccgccgt tctcctgaat ggagcgcctt
     4561 tggtattcgc ccgccgctac gtctgggtga attcactatg ccagagcaca aatttgtcac
     4621 cctggaagat acgccgctga ttggtgttac ccagagctac tcctgttcgc tggagcaaat
     4681 ctctgatttc cgccatgaaa tgcgttatca gttctggcac gattttctcg gcaacgcgcc
     4741 gaccattccg ccggtgctct acggcctgaa tgaaacgcgt ccgagtcagg ataaagacga
     4801 cgaacaagaa gtattctata ccaccgcgtt agcccaggat caggcagatg gctatgtact
     4861 gacggggcat ccggtgatgc tgcagggcgg cgaatatgtg atgtttacct atgaaggtct
     4921 gggaaccggc gtgcaggagt ttatcctgac ggtatacggg acgtgcatgc caatgctcaa
     4981 cctgacgcgc cgtaaaggtc aggatattga gcgatactac ccggcagaag atgccaaagc
     5041 gggagatcgc ccaattaatc tgcgctgtga actactgatt ccgatccgtc gttaacgctg
     5101 cagctcatct aatgcagggg cgtccagatg cgagatgtcc cctgcggttt caacgaccca
     5161 gccagacgcc agccacaggc tttcctgata atccacgcgc gaaatagaac agttacgcag
     5221 acgtaagcgg cgctctgccc atgctggtaa tccgagaatc gtactcacca gacatcccag
     5281 tgcaatacca tggctcacca gcagcggtcg gcttccctgc ggtaagtccc ggcaagattc
     5341 cagtgccgca ttgacgcgat cgctgagctc ctgcattgac tcgccttcag gaatacgccc
     5401 gtcaacggtg ccattgacca gctgccgacg ccagttctct tcttcttcgg tgagtgaatc
     5461 gatatttctt gtttccagta cacccatatt taattcacgc aggcgagaat caaagatgat
     5521 atcacagccg caggcctggg cgatgatttc cgccgtgcgc cgggtccgtc ctaaatcgct
     5581 actgatgata tgcgtaatgc caagctcttt ggcacgggtt gccacctgca tcgcctgttg
     5641 ctcacctttg gcggtcagcg ggctgtcaga ctggccctga atacgtcgct cggcgttcca
     5701 ctgcgtttca ccgtggcgga ctaggtatac ctgtaacatg ctttttttcc gtatactgct
     5761 gtaacgttaa ttttctgagt aatgctgatt atgcaccaag ttgtctgtgc gaccaccaat
     5821 cccgctaaaa ttcaggccat tctgcaggca tttcacgaga tcttcggcga aggatcctgc
     5881 catattgcat ccgttgccgt cgagagcggt gtaccggaac aaccctttgg cagtgaggaa
     5941 acgcgcgctg gcgcacgaaa tcgggtagca aatgcccgcc gtttacatcc agaggctgat
     6001 ttttgggtgg cgattgaagc aggcattgat ggcgacagca ccttcagttg ggtggtgatt
     6061 gaaaacgcca gccagcgcgg cgaggcgcgt tcggcaactt tgccgttacc tgcggtgatt
     6121 ctggaaaaag tgcgtgaagg cgaggcgctc ggtcccgtga tgtcacgtta taccggtatt
     6181 gatgagattg gtcgtaagga aggggcgatc ggcgtgttta ccgccgggaa acttacccgc
     6241 gccagcgtct atcaccaggc ggtgatcctg gcgctgagtc cgtttcataa tgcggtgtat
     6301 cagtgatctt tcagcaacac ctcttccagc cactggcgca gctccacggg cgcggctttc
     6361 aggctgttag atccacgcgt aatcgtcgcg atgcccgcgc cgagttcatt ttttaactca
     6421 cgctggctca tttcgccgcg caacagctct tcgacaatac gcacgcgagt ccccaacgct
     6481 tcgcgctcat ctggcgtcag catcaggttt aacaacggta aatggagatc gttttggtag
     6541 gcattcttaa gcaggtcgac aaaacgtaac cactcctggt gacgctgttc tgccatcgct
     6601 gctgaatagg gtgattgttg ggccataata tgtcgccatt gttagcgggg gaagcaaaat
     6661 gcctcccccg gttgtactcg ctaaagagta cgatagcata tcataaacgt gcggatcagt
     6721 aacgacgtcc ccattccgtg gcgctcatca acgtcggttt atcccccatg aaatagcggt
     6781 agtaagcgtc ataagccagc acgttcttca cataaccgcg cgtctcggag aacggaatac
     6841 tctcgacaaa tgccacagcg tcgatacgcc cggcgctgtt gccaagccag gttcgcaccc
     6901 gtccaggtcc ggcgttataa gctgctgagg agaaaatacg gttattgcca aactgctgat
     6961 aaacatattg caggtaactg gtgccaatgt tgatgttcgt ttccggatcc agcaattgcc
     7021 cagggctgct ataaccagga atagagaaca tcttcaccgt atgggtcgcc gtgcccggca
     7081 taatctgcat caggccgctg gcccctaccg gtgatttcac tttcggattc caggcgctct
     7141 cctgacgggc aatcgccatc gcatagcttt gcgggatctc cttcccactg gtgtaacgtt
     7201 tgaaaagatc gttgtaggcc agcgggaatc tctcttccag atgatcccaa agcttcccgg
     7261 cgatcgttgc ctgaacgcta agttcccacc attggttatt gaaagcatac cgagccagtt
     7321 gcgcctgctc tgtttttgac ttgctcttca ccagattggc ccactcgcta cgcgcggtat
     7381 tatcgagatt ccagtacatc aactcgcgca cgcgcgccat ctccggcccc tgagtcaggg
     7441 cgctgtcaac attctgcggc gctttatcaa tcttcagctc atactcttca ccgatgcgtt
     7501 gtgctgcaac catcgggtag aaaccacgct gttgcatgag ttgatgcaaa atctcttttg
     7561 cttcagcttc acgtccgcgt tccagcagta aatccgcctg ccagtaacgc cattcatctt
     7621 tctctttcgc ctccatcggc aaacgcgcca gccaggtatt caggccgcga cgatcgccgg
     7681 tgccaagcgc cattcgcacg cgccgttcaa taagcgaagt agattgcgaa cgcataatgg
     7741 cgtcatcgcg ccatttcgcc tgctcatcgg tgacatcatt gcccatcaaa cgccaggcga
     7801 cgatatcgcg cagctcctga atctgatctt cattaagctg ctgcgcctgg gcaagcgacg
     7861 ggatcatcag ccgtgcattt tcagcatcct gccgcgccac actggcaaac gccaccgccg
     7921 ccatttgacg ggtaaaatcg gtcgctccgg ttgtacgcgc gaaggtcagt accgtattag
     7981 ggttgttcgc cagtgaaatg attgccgagg cgatagtctg gtaatcggca ggcatctgcc
     8041 ctgccagcac tgttacgagt ccagtgttac ccgctttcat cgccagacgg atacgctcta
     8101 aatacgccag cggatcttgt ttacctgacg cacgccagac gctaaataac ttgtcacagg
     8161 cgttaggctg gctcttgccg gttagccaca gctctttcgc cccttgccag gcttcttcac
     8221 tctgcccggt gttccatttc gcatagtagt aattacattg cgcttcggta gttccgggct
     8281 tttccgggct aaaggctaac aagccacgcc agtcttcacg ccgcgccagt tcattgacga
     8341 aacgagattg cagcgtgcga gcgggaggaa gcgtggggtt agcgcgaaca aagttagtga
     8401 ccgtcaccgc cggttgattc atcaggtcat cggtgatctg acggtattcc aggtagggat
     8461 aaagcggata atctttcagt ccaggcatca tttgttcgac cacatccatt tgtcgattat
     8521 cccaggcctg cttgatttgg gcgtagcggc tacgctgctc atccagtgag tcggctcgcg
     8581 ccacgctgct gaccgtcagc agacagacac cggcagccaa cagccgccag gtaacttgtt
     8641 tggctttttc cacaagcgca tcctctaagt gtaaatacat caatgcagcg tcatgccgcg
     8701 taatagattt atgctaacca gtcattgccg tttacgccac gttacggaca cttttttact
     8761 tttatactct aaataattcg agttgcagga aggcgataag tgagtgagtc cccaggagct
     8821 tacagaagta agtgactggg gtgagcgagc gtaagcgcag cacatgcaac ttgaagtatg
     8881 acgagtatac tgcgagggtg atcgaccggc tgggattaga taaggaaaca ggctacactt
     8941 cgcctttgaa aactcatcat cacgataaac atagaggcga agtccaacgt ggctcaattc
     9001 gtttatacca tgcatcgtgt cggcaaagtt gttccgccga aacgtcatat tttgaaaaac
     9061 atctctctga gtttcttccc tggggcaaaa attggtgtcc tgggtctgaa cggcgcgggt
     9121 aagtccaccc tgctgcgcat tatggcgggc attgataaag acatcgaagg cgaagcgcgt
     9181 ccgcaaccgg atatcaagat tggttatctg ccgcaggaac cgcagttgaa cccggaacac
     9241 accgtgcgtg agtccattga agaagcggtt tctgaagtgg ttaacgccct gaaacgcctg
     9301 gatgaagtgt atgcactgta cgccgatccg gatgccgatt ttgacaaact ggccgctgaa
     9361 cagggccgtc tggaagagat cattcaggct cacgacggtc acaacctgaa cgttcagctg
     9421 gagcgtgcgg cggatgcgct gcgtctgccg gattgggacg cgaaaatcgc taacctctcc
     9481 ggtggtgagc gtcgtcgcgt ggcgttgtgc cgcctgctgc tggaaaaacc agacatgctg
     9541 ctgctcgacg aaccgaccaa ccacctggat gccgaatccg tggcctggct ggaacgcttc
     9601 ctgcacgact tcgaaggcac cgttgtggcg attacccacg accgttactt cctcgataac
     9661 gttgcgggct ggatcctcga actggaccgc ggtgaaggta ttccgtggga aggtaactac
     9721 tcctcctggc tggagcagaa agatcagcgt ctggcgcagg aagcttcaca agaagctgcg
     9781 cgtcgtaagt cgattgagaa agagctggag tgggtacgtc aaggtactaa aggccgtcag
     9841 tcgaaaggta aagcacgtct ggcgcgcttt gaagaactga acagcaccga atatcagaaa
     9901 cgtaacgaaa ccaacgaact gtttattcca cctggaccgc gtctgggcga caaagtgctg
     9961 gaagtcagca atctgcgtaa atcctatggc gatcgtctgc tgattgactc cctgagtttc
    10021 tcgatcccga aaggggcgat cgtcgggatc atcggtccga acggtgcggg taaatcgacg
    10081 ctgttccgta tgatctctgg tcaggaacag ccggacagcg gcaccatcac tttgggtgaa
    10141 acggtgaaac tggcatcggt tgatcagttc cgtgactcaa tggataacag caaaaccgtt
    10201 tgggaagaag tttccggcgg gctggatatc atgaaaatcg gcaacaccga gatgccaagc
    10261 cgcgcctacg ttggccgctt taactttaaa ggggttgatc agggtaaacg cgttggcgaa
    10321 ctctccggcg gtgagcgcgg tcgtctgcat ctggcgaagt tgctgcaagt tggcggcaac
    10381 atgctgctgc tcgacgaacc aaccaacgac ctggatatcg aaaccctgcg cgcgctggaa
    10441 aacgccctgc tggagttccc gggctgcgcg atggttatct cgcacgaccg ttggttcctc
    10501 gaccgtatcg ccacgcacat cctggattac caggatgagg gtaaagttga gttcttcgaa
    10561 ggtaacttta ccgagtacga agagtacaag aaacgcacgc tgggcgcaga cgcgctggag
    10621 ccgaagcgta tcaagtacaa gcgtattgcg aagtaatgcg taaaatgccg gatgcggcgc
    10681 gccagcatcg catccggtat tagttaaatg attaatggga ggcaagttcc cggaaaaagt
    10741 caggattttc tttaattgtg gcaagtactt ttgcagccaa tcccgtagga tttcttcgct
    10801 tcgattccca gcttttaatg gtatccacac tggttcccag agcttttgcc atctcacttt
    10861 gcgaaacgtt gagttgctca cgaatagctt taacatccgc aatttcgtag cgggtcaccc
    10921 gtgcaggttc ttttaaacct tgtttaatct cgacagcctc ttccagagag gttttcaact
    10981 catcaaaaaa actcatttta tatctcgcct ttgagcagtt tcgtcaactt ctttagttga
    11041 gctttttccg tatctgtcag actatctttg gcatttttcg gataggccat aatcaaataa
    11101 ataatctctt ccgtcgccag aaagtaaatt attctgacac tgccactttt tccctgtgaa
    11161 cccgcagcca ttcggacttt acgcaatcca ccagtctgct gaatgagatc acctttatcg
    11221 ggagttccga tgagttcttt ttgtaattcc tttaattcat catcggttgc aatctgcttg
    11281 atctgacgcg taaacattgg cgtctctata aatattatcg ccttacccat tacccctccc
    11341 tggaaggtac attgtacacc aaaatggagt actaagtaca cttttgaatc agtaatccca
    11401 ctcaacatct tataaagatg attatctttg ctcccccatc atctcccgca ccagctccac
    11461 gcagcgcagg aaacggctgt cataatcgtc ttcttcaacc cgcacaaatt cgatattgtt
    11521 ctcttccagc atctccacca gcaagttctg gaactctttg cgatccaccg aactgccgag
    11581 gctgcgtaaa ccatccgcca cccaaggggt gttgttctcc agcaagataa ccagatcaaa
    11641 acggtattca tcgatcagcg cctgcacgaa cggatgctcc cgcccttcgt actttttgca
    11701 gaacgcctgg gtagtgacaa aatcagtgtc gataaacgcc actttattgg catatttcac
    11761 tgcaaaatca atgtattgtg cgtgtcccag cgcgatttta tcgtagtcgg aatactgcaa
    11821 tgcgatctca tcaccgccga ggtgtgaaaa gacataatcg cgaccatatt cccacgcact
    11881 ggtggtattg aagatattgg caagtttgtt taccagggtg gatttaccgc tcgactcgcc
    11941 gccaaggatc gccacggtac gcacaaaaaa cggcttcact tcagtgggga tatattccca
    12001 gtagcggaac gggttttcgc ggatctgcgc accgctgata ctcataaagg tgcgtttcgg
    12061 atcgaccagc accgtttcga tccccagatg ttccatatac tgtggcgcat cggcttcttc
    12121 cgaggtgtag atcaggtccg gctggatccc cttttcagcc ataaactttt tgatgccgtt
    12181 gctccataca tcccagccgt gcggatatgg ctccatgccc tcttcattga aagcatgaat
    12241 acggatattt ttctgatatt taaaggtttg caataaccag cgcagacgat ccggcaccgt
    12301 gggctgctgc gacatggcgc tgtcttcgaa caacgcgcgg tcgcgggtat cgtcaaagcc
    12361 cataatgata tgcagctcgt caacctggct acaggcgcgc tggataaggt agatatgtcc
    12421 ggtatgcagc gggtagaact taccgaatac gacaccaatg gttttcttct gtcggggaaa
    12481 ttcaagcccc aaaaaacggt gcaacgcctc cagcttttgc gcgctggggc ttttaatttt
    12541 ggcattcagt aactggctta aatacccttt ggtcataccg ctggcatcag cgacctgctg
    12601 tagcgtgcag ccctgttgct tgatggcagt tttcaggtaa tcaaatgacg acatatctcc
    12661 ctccgtatct ctcattataa gtcgtcgaac acgctaagcg cgtcggagag ttttttaacg
    12721 ccaaaaatct gcatcccttc cggcgctttt ttcggcacgt tagccgccgg aacaatcgcc
    12781 cggcgaaaac cgtgtttcgc cgcttcagaa attcgttcct ggccgctggg caccgggcgg
    12841 atctcccctg ccagcccgac ttcaccaaac accaccagat cctgcggcag cggtctgtcg
    12901 cgcaggctgg aaaccatcgc cagcagtaac gctaagtcgg cgctggtttc cgttaccttc
    12961 acgccgccga ccacgttaac aaatacatcc tgatcggcca tttgcaaccc accgtgacgg
    13021 tgcagcaccg ccagcaggat tgccagacgg ttttgctcca gccctactgc gacgcggcgt
    13081 gggttcgcca tcatcgagtg atcgaccagc gcctggatct ccaccaacag tggacgcgtt
    13141 ccttcccaca ctaccatcac tgagctaccg gaggtcactt catcaccgcg gcttaaaaag
    13201 atggcggaag ggttgctaac ttcacgcagc ccctgttcgg tcatcgcgaa gacgcccagc
    13261 tcattcaccg cgccgaagcg gtttttatgg ctgcgtaagg tgcgaaaacg agagtcggca
    13321 tcgccatcca gaagcaccga acagtcgata cagtgttcca gcactttagg gccagccagc
    13381 gagccatctt tggttacgtg cccaaccatg acaatcgcca cgccgcgcgt tttggcgaag
    13441 cgcgtcagat aagccgccgt ttcacgcacc tgcgccacgc tgccaggcga tgactgaaca
    13501 tccgccatat gcatcacctg gatggagtca attaccatca gcttcggttg ctcttcttcg
    13561 gcaatcaggc agatctgctc gatgctggtt tccgacagca tattaagatt gtcagtcggc
    13621 aggccaaggc gatgagcgcg cattgccacc tgttgcagcg actcttcgcc agtgacatac
    13681 agcgttttca tctgctgcgc cagtttgcac agcgtttgca gcaacagcgt ggatttcccc
    13741 gcacctgggt taccgccaat cagaatggcg cttcccggca ccaccccgcc gcctaatacg
    13801 cggtcgaact ctttaaatcc ggtggaaaaa cgcggcagct cctcaaggct gatgtcggaa
    13861 agtttctgga cttttgccac tccggcgcta ccggcatagc cactgagacg ctcgttacgc
    13921 gccaccgttg gcgacgcagc aagacgcacc tcggtgatgg tgttccaggc atgacaggcg
    13981 ctgcactgcc cctgccagcg cggataatcg gccccgcatt cattacaaac aaaggcgcgt
    14041 tttggagctt ttgccacggt gtacctcgtt aatgctgtgc cgcccgcagg atgacgggca
    14101 ggcaattact tctgattcag gctgccagag aggatgcaga atacccccat caggtcagcg
    14161 tgacggatgg tgacttccgt cttttcattc acttttggct tggcatggta ggcaatcccc
    14221 agccctgccg ctttgatcat cggcaggtca ttggctccat cgccaatcgc cacggtctgc
    14281 gccagcggga tttcatactc ctgcgccagg cgggtcaggg ttttcgcttt atattgcgcg
    14341 tcaacgatgt cgccgatcac attgccggta aatttaccgt ccatgatctc cagttcattg
    14401 gctaccactg ctgtcaggcg cagcttgtcg cgcaggtatt cagcaaagaa agtaaagccg
    14461 ccggaggcaa tcgccacttt ccagcccagc gtttccagct tgagtaccag ctgcgttaag
    14521 cctggcatca gcggcagatt ttcacgcacc tgttgcagaa tattggcgtc agcgcctttc
    14581 agcgtcgcca cgcggctgcg caggctggcg gtaaaatcga gttcgccgcg catcgcccgt
    14641 tcggttacct ccgccaccct ctcgcccgtt ccggccagtt tggcaatttc atcaatacat
    14701 tcaatctgga tggcggtgga atccatgtcc atcaccagca aacccggcgt gcgcaggtgc
    14761 gggattttcc ccagcggcgc gacatccagc tgcgcttcgt gggccaggcg cgtagcccgc
    14821 gcggtgagtg aacctgccag acgaatcacc tgataatctt ccacgcacca ggcggcaaca
    14881 atcaccatcg ccgcgcccag tttgctctgg tattgggtca gacgttgttt atccagccca
    14941 cgaccataca gcagccagcc gctacgacct gcgtggtaat ccagtggcat cacttcatca
    15001 ccacttaatg aaagaggcag acccggccat aaagagacat cttcaggcag gtcgcaccag
    15061 gtaatgttag gcattaaggc tcctgtaaaa tcgttcgaag cagggaaaat aacgcatgag
    15121 gctaccttgt atccattgct tctggcaaca ttaagtctca aattttcaaa gggtggaaga
    15181 tggctcgcac aaaactgaaa ttccggctgc atcgggcagt gattgtcctg ttctgtcttg
    15241 ccttgttagt ggcgctgatg cagggagcgt catggtttag tcaaaaccac cagcgacagc
    15301 gtaatccaca gctggaagaa ctggcccgca ccctggcgcg tcaggtgacg ctgaacgttg
    15361 caccgctgat gcgtaccgac tcaccggatg aaaaacgcat tcaggcgatc cttgatcagt
    15421 taacggatga aagccgtatc ctcgacgcgg gcgtgtatga cgaacaaggc gatcttatcg
    15481 cccgttctgg cgaaagcgtc gacgtgcgcg accggttggc gctcgacggc aaaaaagcgg
    15541 gcggctattt taaccagcag attgtcgaac caattgccgg gaaaaacgga ccgctcggct
    15601 atctgcgcct tacgctcgac acccatacgc tcgccaccga agcccaacag gtggataaca
    15661 ccactaacat tttacgcctg atgttgctgc tctcactggc aatcggcgta gtgctgaccc
    15721 gcacgctgct acagggtaaa cgcacccgct ggcagcaatc gcccttcctg ttaaccgcca
    15781 gtaaaccggt gccggaagag gaagaaagcg agaaaaaaga gtgacccatt actacaagaa
    15841 aggaaatcgt tatgtccaca ttacgcctgc tcatctctga ctcttacgac ccgtggttta
    15901 acctggcggt agaggagtgt atttttcgcc agatgccggc tacgcagcgc gttctgtttc
    15961 tctggcgcaa tgccgacacg gtagtgattg gtcgcgcgca gaacccgtgg aaagagtgta
    16021 atacccggcg gatggaagaa gataacgtcc gcctggcgcg gcgcagtagc ggtggcggcg
    16081 cagtgttcca cgatctcggc aatacctgtt ttacctttat ggctggcaag ccggagtacg
    16141 ataaaaccat ctccacgtcg attgtgctca atgcgctgaa cgcgctcggc gtcagcgccg
    16201 aagcttccgg gcgtaacgat ctggtggtga aaaccgccga aggcgatcgc aaagtctcag
    16261 gctcggccta tcgcgaaacc aaagatcgtg gcttccacca cggcactttg ctgctcaatt
    16321 ccgaccttag ccgcctggca aactatctca atccggataa aaagaaactg gcggcgaaag
    16381 gcattacctc agtgcgttcc cgcgtgacca atctgaccga attgctaccg gggatcaccc
    16441 atgagcaggt ttgcgaggcc ataaccgagg cctttttcgc ccattatggc gagcgcgtgg
    16501 aagcggaaat catctccccg gacaaaacgc cagacttgcc aaacttcgcc gaaacctttg
    16561 cccgccagag tagctgggaa tggaacttcg gtcaggctcc ggcattctcg catctgctgg
    16621 atgaacgctt tacctggggc ggcgtggaac tgcatttcga cgttgaaaaa ggccatatca
    16681 cccgcgccca ggtgtttacc gacagtctca accccgcgcc gctggaagcc ctcgccgggc
    16741 gactgcaagg ctgtctgtac cgcgcggata tgctgcaaca ggagtgcgaa gcgctgctgg
    16801 ttgacttccc ggaacaggaa aaagagctac gggagttgtc agcgtggatg gcaggggcgg
    16861 taaggtaact cgctgattaa aatatccagg gaaggaaata tgatcgatct ggaaaaccag
    16921 gaacgggaga ttatcaattt aatgttcagc cagggtatat cctggctaac cgccgtgcgt
    16981 atcaggcata aactatcact tgctgaagta agtaaaatgt taggtatctc cattaattct
    17041 ctaaaacaga tcgaaaagac ggaacggctt agttcaaaca tcaaaagcaa aatggcagga
    17101 atttatggtt gtccaccaga gttgctgata tgcccttcct ggatgacggc tgaacataaa
    17161 tgaatagatt cgtgaataga tacttgaatg gattttattt tctatccata tgaaaaataa
    17221 tagtaaatct ataaatcagt tgctattgat gaataaatac taaaaagccg gagcagtctc
    17281 ccactccggc ttcacacgac aatcgccttt cagcaaaaca caattactct ttatcgccca
    17341 gcagaacgga ttccagcgcg attttgatca tgtcgttgaa ggtggtctga cgctcagcgg
    17401 cagtggtctg ctcgtgagtg cggatgtggt cagacacggt gcagatggtc agggctttcg
    17461 cgccaaattc tgcagcgacg ccgtagatac cagccgcttc catttccacg ccgaggatgc
    17521 cgtatttttc catcacgtcg aacatttcgc cgtccggaga gtagaacagg tcagcggaga
    17581 acaggttacc aacgcgagca tcaacgccca gtgcttttgc cgcgtctacc gcgttacgca
    17641 ccatgtcgaa gtcagcgata gcggcaaagt catggtcttt aaaacggatg cggttaactt
    17701 tggaatcggt gcaggcaccc ataccgataa cgacgtcacg cagttttacg tgcggcagaa
    17761 cagcaccaca ggaacctacg cggataattt tcttcacgcc gaaatcggtg atcagttctt
    17821 tggtgtagat ggagcaggac gggataccca taccgtgacc cattacggaa attttgcggc
    17881 ctttataagt accggtgaag cccagcatac cgcgaacgtt gttcacttca cgggcatctt
    17941 caaggaaagt ttcagcaata tacttcgcac gcagcgggtc gcctggcatc aaaactacgt
    18001 cagcaaaatc gcccatttct gcattaatgt gtggagtagc catcgtttta tcctttttgt
    18061 gacatattaa aggcggagtc actgctccgc ccaaatccat cagaacatgg ctttgccata
    18121 ttccatatca gaagtaccaa aatattttgc cagagtctgg ccgatatccg cgaaggtttc
    18181 acgatgcccc agcgaacctg gtttcacttt cgggccgtaa accagtaccg gaatgtgttc
    18241 acgcgtgtgg tcagtaccgg tccaggtcgg gtcacaaccg tggtcagcag tgagaatcag
    18301 aatatcgtca tcgcgcagca aagacatcag ctccggcaga cggcggtcga acagttccag
    18361 acccgcagca taaccggcga cgtcgcgacg gtggccccag gaagagtcga agtcaacgaa
    18421 gttggtgaag acgatggtgt tatcacccgc ttctttcatc tctttgatgg tggcgtcaaa
    18481 cagcgcgtcc aggccggtcg ctttcacttt tttggtgata ccgcagttgg catagatgtc
    18541 tgcaatttta ccgaccgaaa ccacctggcc gtgtttttca tcaaccagtt tctgcagcac
    18601 ggtcggtgct ggcggctcaa cagccaggtc gtgacggtta ccggtacgct ggaagttacc
    18661 ggctttgtcg ccgataaacg gacgagcgat aacacgacca atgttgtagc cgccgttggt
    18721 cagctcttca cgggcgattt cgcacagttc gtagagttta tccagaccaa aggtttcttc
    18781 gtggcaagca atctggaaca cggagtcagc ggaggtatag aaaatcggct tgccggtttt
    18841 catgtgctct tcgcccagtt gatccagaat gaccgtaccg gaagagtggc agttaccgag
    18901 gtaacccggc agattagcgc gttcaaccag tttatccagc agctcttgcg ggaagctgtt
    18961 ttcgtgatcg gagaaatatc cccactcaaa cagaaccggg acaccggcga tttcccagtg
    19021 accagacggg gtgtctttac cggatgacat ttcgtgcgcc catgcgtacg cgccgataac
    19081 ttcagcgttg ccatccattc ccgccggaat gaaaccggta gaaccttcgt gtgctttcgc
    19141 cagcccaaga cgggtcagat ttggcagatt gagcgggcct ttacgaccgt ggtcagcttc
    19201 gcctttggca caagcttctg cgatatgacc cagggtgtca gccccgacgt caccaaagcg
    19261 ttctgcatct tctgtagcgc cgatgccgaa tgagtccagc accataataa atgcacgttt
    19321 catatgttct ccgtactcag tgcttcaaat ttaaatgatc agatcagtat accgttattc
    19381 gctgatacgg cgatagacag ttggtgtgct ttccggtgct ttatcggcaa gtttaattgc
    19441 cgctttcacc gctttcgccg cttcctgcca gctgttttcg tctttcgcgt ggataactgc
    19501 cagcggacgc tgaccgtcta cctggtcgcc cagacgcgcc atatcagtaa agccgacgct
    19561 gtaatcgatg gtgtcagatg cctgacggcg tccgccgccc attgcaacca ctgccatccc
    19621 cagtgcgcgg gtatccattt cactgacaaa cccttcggta tcagcataga ctgctttcgt
    19681 cagcatcgct gtcggcagat acttcgcgta gttctcaacg aagtcggtcg ggcctttttg
    19741 tgccgcgacc atacgaccaa agacttctgc cgctttaccg ttgtccagca ccgcctgcaa
    19801 tttcgcgcgc gcttcggcgt catttttcgc cagtttgccg gagatcagca tctccacgca
    19861 cagtgccatc gtgacatcaa acagacgcgg gttacgatat tcacccgtca ggaactgcac
    19921 cgcttcacga acttcaaccg cgttacctgc actggaggcc agtacctgat tcatatcggt
    19981 gagcagcgcg gtggtgcgca cgccagcgcc gttagccacg ccaacaatcg cttcggcaag
    20041 ggcttcagag agttcgtagg tcggcataaa cgcgccgcta cctactttca cgtccatcac
    20101 cagcgcgtcc agaccttccg ccagtttctt cgccagaata gaggcagtga tcagcgggat
    20161 ggagtccacg gttgcggtaa tatcacgggt cgcgtagaaa cgtttatccg ccggagccag
    20221 tgagctggtc tggccgataa tcgccacgcc gacgtcttta ataatttcgc ggaaacggtt
    20281 gtcatccggg aaaatgtcga agccagggat ggattccagt ttgtcgagcg taccgccagt
    20341 atgaccgagg ccgcgaccgg agatcatcgg aatatagccg ccgcaggctg cgaccatcgg
    20401 ccccaacatc agcgaagtga catcgccgac gccgccggtc gagtgtttat caacaatcgg
    20461 gccattcaga tgcaggcttt tccagtcgag aacggttcct gaatctcgca tagccatggt
    20521 cagcgagaca cgctcaggca ttgtcatatc gtggaagaaa atggtcatcg cgagggcggc
    20581 aatctgccct tcggagatag tgttgtcgcg aataccgttg ataaagaaac gaatttcttc
    20641 atcgctcagc gcatgaccat cacgtttttt acgaataatt tcttgtgcga gaaacaaggt
    20701 aacctcccgt ggaaaagaga cgaggtaatc ggggaaccgg atgagcatcg ctgcatcatc
    20761 cggcaaatca agccttagta gctgctggcg ctcttaccgt cgccgtggcc cagagctttc
    20821 agcaggcttg ccagcaggct ggaagcacca aagcggtagt gacgcgcatc agcccagtca
    20881 gcaccgaaca gttcatcggc gatggcgaga tatttctgcg catcttccgc agtacgcacg
    20941 ccgcccgccg gtttgaaacc aacggttttt tctacgccca tatcacggat cacttccatc
    21001 atgatgcgcg cgctttccgg cgtcgcgttc acagccactt taccggtaga ggttttgatg
    21061 aagtccgcac ccgctttgat ggagatttca gacgctttac ggatcagcgc ttcgtctttt
    21121 agttcgccgg tttcgatgat cactttcagc agtacattcg ctgccgcgca agcctcttta
    21181 caggctttca ccaggtcaaa accaacctgc tcgttacccg ccatcagcgc gcggtacggg
    21241 aacacaacgt caacttcatc agcaccgtag gcgattgccg cacgggtttc tgccagcgcg
    21301 atttcgatgt cgtcgttacc gtgtgggaag ttggttaccg tagcgatacg gatttccggg
    21361 gtaccctgct ctttcagcgt tttgcgagca attgggataa agcgaggata gatacagata
    21421 gcggcggtat tgccgaccgg agttttggcc tgatgacaca gagcaattac tttctcgtcg
    21481 gtgtcgtcgt cattcagggt ggtcaggtcc atcaatttca gtgcacgcag gctgcttgct
    21541 ttcagatcag tcatttcatt ctcctggctt gtcgccaata aaattcacct tgcgagtttg
    21601 ttagtattct aacatctact ccgcaacaca cttcgatata catcacaatt aaggaaatct
    21661 acttacaagt ttgcatcact gtaatgcgat ctggttcaaa taattcactt tcaaatgaat
    21721 gcgtcagtgg tggcaaacgc atcaggatca aagtgaacat cacgaaactt cttacaatgg
    21781 cgcatcacct tcggcataaa aggaaaagaa gatgcccact tctcatgaaa atgcactgca
    21841 acaacgttgc cagcaaattg tcaccagccc ggtacttagc ccggagcaga agcgccattt
    21901 tctggcactg gaagcagaaa acaatctgcc ttacccacag cttcctgccg aagcccgccg
    21961 cgcgctggat gaaggtgtaa tctgcgatat gtttgaaggt catgcgccgt acaaaccgcg
    22021 ctatgtctta cccgattacg cccgttttct ggcgaacggt tccgaatggc tggagctgga
    22081 aggcgcgaaa gatcttgatg atgcactctc tctgctgacc attctttacc accacgtacc
    22141 atcggtcaca tcgatgccgg tctacctggg gcaactggat gcgttgttgc aaccgtatgt
    22201 tagaattcta acacaagacg agatcgatat tcgaataaaa cgtttctggc gttacctcga
    22261 cagaaccctg ccagacgcct ttatgcacgc caatatcggc ccgtctgatt cgcccattac
    22321 ccgcgcgatc ttacgtgcag acgcagagtt gaagcaggtt tcaccgaacc tgacctttat
    22381 ctacgatcct gaaatcaccc ctgatgacct actgctggaa gtggcgaaga acatctgtga
    22441 atgtagcaaa ccgcacatcg ccaacggtcc ggtgcatgat aaaattttca caaaaggggg
    22501 ctacgggatt gtgagctgtt acaactcact gccgctggcg ggtggtggca gcacgctggt
    22561 acgccttaac ctgaaagcca ttgccgagcg cagtgaatcg ctggatgact tctttacacg
    22621 cactctaccg cactactgcc agcagcagat cgccatcatc gatgcgcggt gtgaattcct
    22681 ctatcaacaa tcacacttct ttgagaatag cttcctggtg aaagaagggc tgattaaccc
    22741 tgaacgtttt gtgccaatgt ttggcatgta cggattggcg gaagcagtta acttgctgtg
    22801 tgagaaagaa gggattgccg cgcgctacgg taaagaagcc gccgcaaatg aagtgggtta
    22861 tcgcatcagc gcacaactgg cggagtttgt cgccaatacc cccgtgaaat atggctggca
    22921 aaaacgcgcc atgctacacg cacagtcggg gatcagttcc gatatcggca ccacgcctgg
    22981 cgcacgcttg ccgtatggtg atgagccaga tccgattacc catctgcaaa ctgtcgcccc
    23041 gcatcatgct tattattatt ccggcatcag cgacattctg acgctcgacg aaaccatcaa
    23101 acgtaatccg caggcgctgg tacagctttg cctcggtgcc tttaaagccg gaatgcgtga
    23161 atttaccgcc aatgtcagcg gtaacgatct ggttcgcgtt accggttata tggtgcgttt
    23221 gtcggattta gaaaaatatc gcgccgaagg ttcacgcacc aacaccacct ggctgggaga
    23281 agaagccgca cgcaacactc gtattctgga acgccagccg cgcgtgataa gccatgaaca
    23341 gcagatgcgc tttagtcagt aagattatcc ccttctcctg cgttgacggg ccaggcagtc
    23401 gtctggctct gtttttgcag ggctgcaatc tgcgctgcaa aaactgtcac aatccgtgga
    23461 cgatggggcg ctgtaatgat tgcggagagt gcgtgccgca gtgtccgcat caggcgttgc
    23521 agattgtcga cggcaaagtg gtgtggaacg ctgtggtctg cgagcaatgt gatacctgcc
    23581 tgaagatgtg tccgcaacat gccacgccaa tggcgcagtc catgagcgtg gatgaggtgc
    23641 ttagccatat ccgcaaagca gtgctgttta tcgaagggat aacggtaagc ggcggcgaag
    23701 ccacgaccca gctaccgttt gtggtggcgc tgtttactgc tatcaaaaac gatccgcaac
    23761 tgcgccacct cacctgtctg gtggacagta atggcatgtt gagcgaaacc ggctgggaaa
    23821 aattgcttcc ggtgtgcgac ggcgcaatgc tcgatctcaa agcgtggggg agcgaatgtc
    23881 atcagcaatt aaccggacgc gataatcagc agatcaagcg cagcatctgt ttgctggcag
    23941 agcgcggtaa gctggcggaa ctgcgtttgc tggtaatccc tggccaggtg gattatttgc
    24001 aacacatcga agaactggcg gcgttaatca agggacttgg cgatgttccg gtacgcctga
    24061 acgcgtttca tgcccacggc gtgtatggcg aggcgcaaag ctggccgagc gccacgccgg
    24121 aggatgtgga acaactggct gatgcgttat gggagcgcgg ggtgagccgg ttaatatttc
    24181 cggcgctata tttgtgacat gccggatacg gcctacggat cggcacagac ctgtaggcat
    24241 gataagatgc gtcaagcgtc gcatcaggca ttgtgcgcca gctgccggat gcggcgtgaa
    24301 cgccttatcc ggcctacggt tcggcacaga cttgtaggca tgataagacg cgtcaagcgt
    24361 cgcatcaggc attgtgcgcc aactgccgga tgcggcgtga acgccttatc cggcctacgg
    24421 cacgttaaac aacgtatacg tattatttag caacacttcg gcaatctcat cttctggttc
    24481 cgggcgtaac tcgcaaagta cgtcaaacac ccgcgcagcc tgttccgggc ggttaggctg
    24541 cccctgaaag ccgttgagcg gcatatccgg cgcgtcggtt tccagcaaca acgatgccag
    24601 cggtaatttt gcgatgacat cgcgggtttt actggcgcgt ggataggtga tagtaccgcc
    24661 tacgccgatt ttgtagccca gcagtacaaa cctttcggcc tgttgcaggc tgccggaaaa
    24721 accgtgaacc acgccagtgc gtggcaaatc gtggcgttta agatgcatcg ccagtttgtc
    24781 gtgtgtgcgc cgtgaatgca ggatcaccgg cagatcgtag cgtttcgcca gtttcagttg
    24841 ttcgtcgagt aaccactgct gcctctcaaa ttgcggatcg tcgccgaaga gatccagacc
    24901 gatctccccc accgccacca ccttcgccgg acgcctttcc agcgcctgct gtagctgatc
    24961 aagagacaca tcgctatgtt ttttcaacat accaggatgc aagcccaatg cggcatacag
    25021 cggttgatat ttttccgcta acgcctgcac gcgggcaaaa ttttccgcct ctgtcgccgg
    25081 aacaatgatc ttgcctacgc ccgcttgtgc cgcgcgttgc aggctggcct cttcatcacc
    25141 actaaaaggc gggaaatcaa aatggcagtg ggtgtcgata aaacggcaaa tcaagccaga
    25201 tcctcattgt taaatgtcgg gtcgttcgcc tgcggagcgt cgatcacttc ggcaaccagc
    25261 gtatcgttgg cgactggcgc aggcggaatg acaatcgatt ccggcgtcac caccggcacc
    25321 agatggcggg taagcggcgc tttttcagtc aataacttac cgaccgttgc cagaaaataa
    25381 cgaccgcata aacgcccaag tttatagtct tcacgcagcg ccggaatccg actgccaagc
    25441 gcgatactat gcaacggctt cggcggataa atttcgaata tccgcagctg gcccggtggt
    25501 ttctcaataa attgctgaat gtcacgatag ctggtttcat gatgctgcac cagattgacc
    25561 agcggctgca ggctactgtc acccagccag cgttccatgc gtttgaacca ctgcggcgtg
    25621 tagtacattt gcgacggcac ggtgcggatg acgaccaagg tttttgctcc ctgccttgcc
    25681 gcctctttaa ccggaatcgc atcactgatc ccgccatcca ggtagttaat gccttccagc
    25741 gacactccgc tacgataaaa gccaggtatc gcactggagg cgcgaatcac atccagccag
    25801 ttttgtttgg ttggtaaaaa gtaattcggc gcatagtcat cctgacgaca ggcgcacata
    25861 taaaacgatt tgccgctgtc aaacaaccgc gcggcggtat ccatttgcaa cggcatctgg
    25921 cttgcagtgg cctccaccag ccagtcgaga tcgataagat ttcctccacg gacaaagcgc
    25981 aatggatcga aaaattcgcg ttttgtggta tagcgcatga tgactttgcg cgcgtaaccg
    26041 ggctgattgc agatatacgc cgagaggttc tgcgccccgg cagatgtgcc gagataaaga
    26101 tcgaaaggat taaactgcgc gcgcataaac tcatccagca cgccagccgt gaaaattcca
    26161 cgctgtccgc cgccttcgca caccagagct attcgtccag gctggaacgg ccttagcgac
    26221 aacggcgcaa tattaccaag cgttacaggt attcgctgcc ccacctctgc cttcctgttt
    26281 ttgttgtgaa agaatgacaa agtaacgcaa tttattattc gctaaaaccg caaaagccag
    26341 tccgctggac tggctttgga taaaattttc ttaatggtct gaaatctagg gtcgttttcg
    26401 gcccatgaac aaactcacca ggaacagaat aatcccgacg acaaagacaa ttttagctgc
    26461 gcctgcagcg gtaccggcca gaccaccaaa cccaagtgcg gcggcgatta acgcgataac
    26521 cagaaatatg atgccccaac gaaacataag cgtctccttt accatagtta atgtcaccgc
    26581 ttgatatgag cgtgaaaatc actcaatgcg atatttttag tgtggtgcac atcgcgcctc
    26641 ccgacaaagt tcgggaggac gaattacgac gaattactta gttttcagat catttttaac
    26701 gcttttcaca ccatctaccg ctttggcgat actttcagca cggtcgctct gtgcctggga
    26761 atcgacggta ccggagagct gaaccacgcc gtcggtggtt tcaactttca catgacggga
    26821 agggacgata tcgtccgcca gcagtttggc tttgatttca ctggtggtgg cggtgtcacc
    26881 cgcgtagccc ttcaccgagc cttctttagc gtcgcgaacg tgcagtttgt cgctgacaga
    26941 ggtcacccct tcaacgcctt tcgccacttt cactgcctct tcggcctgag cctggctttc
    27001 aacgaaaccg ctcagggtca cgactttttg atcggttttg acagagatat cggtgctctt
    27061 gatgttgtca tgatccacca gcgccgcctt cactttcgcg gtgatggcgc tgtcatccat
    27121 gaaattaccg actttattca tagagctatc gactttttgc cctgcgcttt cattggtagt
    27181 ctgtgcgttg ttttccgcgt aggcagagcc ggtcgcgacg gcagaggtca acattacagc
    27241 cagcagagtt ttcgaaatct tcagtcttgt catagtcatc gatttattcc tgtatgtttg
    27301 ctcgtaattt gagcctggca acacgaggtt gcattgctga atagggagag acttcaccct
    27361 ctacagaagt caatggccgc catcacaaaa gcgatgggtg atgaataacg accattacag
    27421 cctctgaatc agttattaat atcggtagaa tgacaatcga cggctttaga tactgatatc
    27481 tacgcattga acggtattta acgccgtcag aaatgtcatt actttgttaa atatagatca
    27541 caattttgaa accgctcggg atatcagcga aaacataagc aaaagtgaat gttttaagaa
    27601 cattccgtaa gcggctaata aggaagggaa attgacaggg cgcagcggct accgcgccct
    27661 ggaggcaaga attaatgctc gcgggtctgg tggaactgaa cgtctggata acgttcctgc
    27721 gccaggcgca ggttgaccat gctggtagcg atgtaagcga ggttatcgcc gccatcaagc
    27781 gccagttggc tttcgttctt acgcttgaac tcttcgaatt tcttcgcgtc cgcacattct
    27841 acccagcggg cagtggcaac gttgactgac tcatacactg cttcaacgtt gtattcgctc
    27901 ttcagacgcg ctaccaccac atcaaactgc agcacaccaa ccgcaccaac gatcagatcg
    27961 ttgttggaga tcggacggaa cacctgcacc gcgccctctt cggaaagctg taccagccct
    28021 ttaagcagct gtttttgctt cagcggatct ttcaggcgga tacgacggaa cagttctggc
    28081 gcgaagttcg gaataccggt gaacttcatc atctcaccct gggtaaaggt gtcgccgatc
    28141 tgaatggtgc cgtggttgtg cagaccaagg atatcgccag gatacgcttc ttcaacgtgc
    28201 gaacggtcac ccgccataaa ggtcagcgcg tcggagatca ccacatcttt cgcagtacgc
    28261 acctggcgca gcttcatgcc tttttcatat ttaccggaca ccacacgcat aaacgccacg
    28321 cggtcgcggt gtttcgggtc catgttggcc tgaattttaa atacgaagcc ggtaaatttg
    28381 tcttcgctcg cctctacggt acgggtatca gtctgacgcg gcatcggcgc aggagcccac
    28441 tccaccaggc catccagcat atgatcaacg ccgaagttac ccagcgcagt accgaagaat
    28501 accggagtga tttcgcccgc aaggaacagc tctttgtcga actcgttaga cgcgcctttc
    28561 accagctcca gttcgtcacg tagctgctgt gccagatctt caccaaccgc agcgtcgaga
    28621 tccgggttat tcagcccttt aacaatgcgg acttcctgaa tcgtgtggcc tttaccgctc
    28681 tgatagagat aggtttcatc tttataaagg tggtaaacgc ctttaaacag cttgccgcag
    28741 ccaatcggcc aggtgatcgg tgcgcagccg attttcagct cgttctcaac ttcatcgagc
    28801 agctccatcg ggtcgcggat atcacggtca agtttgttca taaaggtgag gatcggcgtg
    28861 tcgcgcagac gggtaacttc catcagctta cgggtacgat cttcaacacc ttttgcggcg
    28921 tcgataacca tcaggcagca gtccaccgcc gtcagggtac gataggtatc ttccgagaag
    28981 tcttcgtgcc ccggggtgtc gagcaggtta accaggcaat cgtgatacgg aaactgcatc
    29041 acagacgtag taatggagat cccacgctgc ttttccatct ccatccagtc cgacttagcg
    29101 tgctggttgg aaccacggcc ttttactgta ccggcgctct gaatggcctg tccgaacagc
    29161 agcaccttct cggtgatggt agtcttaccg gcgtccgggt gagaaataat ggcaaaagtg
    29221 cggcgcttcg ccacctcttg caaataagga gacaacgtca taattctttc ttcttgagta
    29281 agcggcatcg cgccgcgctt gttgaaaacg aaaaattgcg gctattttac ccatcaacgg
    29341 gggggaggca atcagtgttt acacaggagc tgctccagtt cgtgcaacga agaaacggtc
    29401 caggtgggcg cgatgccttc tggttgctcg cgattgtgcg cattcagcca gcaggtcgca
    29461 agcccggcgt tgatgccacc gagaatatcg gactcagctg tgtcgccaac catcagcaca
    29521 cgtgaacggt caggattgcc tgcctgttcc agcgcataat cgaaaatttt cttattcggt
    29581 ttggcaacgc caacttcttc ggaaatcacc agcaaatcga aataatcacg caggcccgtg
    29641 cgttccagac gcacttgctg caaggcgcta aagccgttgg tgatgatgcc aattttagcg
    29701 ttgccacgaa tggcgttaag cagagaaacc gcgcccggca gtggcgtaca gatttccgcc
    29761 atcgcattaa taaaggcttc gttgagttta cccggctcga cgttcagccg ttcggcccag
    29821 ctctcaaacc gcccgtgctg aagctgtaat gaagtgatcg cgccgttttg ataatccacc
    29881 cacagtggct tgttaacggc ctgatagtcc tgaaaatctt cagcggtaaa ggtgacgctg
    29941 taatcaagaa acatccgctg caagccggtg aatgagtcaa aggtaaacag cgtttcatcg
    30001 gcatcaaaga aaatccagtc ccacttcatt attccacctt gtcttacata ctgattggca
    30061 acgccatgat gatggcgtct tcgcgaccgt ccgtggtggg gtagtaattg cggcgaattg
    30121 tcgcctcgtt aaagcctaaa ctttcgtaca gggcaatggc ggcagcgttt gaagcacgga
    30181 cttccagcca tagcgtcgcc acgccgcgtt tttccagttc gtcgatcaga tgttccagca
    30241 gcgcccttcc caatccctga cgctgatagt caggatcgac cgcaatattg aacaatgtag
    30301 cttcatccag caccacttgc gtaatcgcaa acgccgccat tttgccgttt tgcgttaact
    30361 gaaagttgag ataacgctcg ccctggttgc tggcaaaggt tttttcactc cacggaaagg
    30421 cgtgggcgcg ttgttcaatg tggtaagccg ctggtaaatc agtcgtttcg agggaagaaa
    30481 tcgtgttcat atgtgcaaat ttgttgccat aacgcggcgc gtgccgttgg gtttgcccgt
    30541 aattcggtga gcgccggtga tgccacctga gcgccttcca gtgatagcgg ttcgtcagta
    30601 cccaaccgcc agctgttgca gcgactgcct tgcggcagca tcgcgatttt ttctggcgtc
    30661 agttgcagca cttgatcggg gctgacggtt aacgcgcgca gaacatcgct cactatagga
    30721 tcggtcaggg cgggaagatc gtttgccacc atcaccagac ggacgtgtgc cgggatcgca
    30781 atggcaatct ccccctgcaa cgcgccaggg cgacgcagcg accactgggt aatgcccagt
    30841 tgctgtaact gccagtctcg tcgggatgtc atagcgaatc gctcctgttg tcaggggcgc
    30901 aaatatagca aattcgtcga taccgcgcca acatatggct ataatcgccg ccagtattaa
    30961 ttgaggagca ttccatgtct gcatttaccc cggcaagtga agtcttgctg cgtcacagtg
    31021 atgatttcga acaaagccgt attctttttg ccggagactt acaggatgac ctgcccgcgc
    31081 gtttagatac cgcggccagc cgtgctcata cccagcaatt ccaccactgg caggtgttaa
    31141 gccgccagat gggggataac gcccgtttta gtctggtcgc cacggcggat gatgtcgcag
    31201 attgcgatac gctgatttac tactggccga agaacaaacc ggaagcccag ttccagttga
    31261 tgaatttact ttctctgctg ccagtgggca cggatatttt tgtcgttggc gagaaccgca
    31321 gcggcgtgcg cagcgccgag cagatgctgt cagattatgc gccgttgaat aaaatggaca
    31381 gcgctcgtcg ctgtggcctc tattttggtc gtctggaaaa acagccagta tttgatgccg
    31441 ataaattctg gggcgaatac agcgtcgatg gcctgacggt caaaacgctg cctggcgtgt
    31501 ttagccgcga cggtctggat gtcggtagcc agttgctgct ctcgacgtta accccgcaca
    31561 cgaaaggtaa agtgctggat gtcggctgtg gcgcgggcgt actttcggtt gcctttgcgc
    31621 gccattcgcc gaaaattcgt ctcactctgt gcgatgtctc tgcaccagcg gtagaagcca
    31681 gccgcgcaac acttgcggcc aacggtgttg aaggtgaagt ctttgccagt aacgtctttt
    31741 ccgaggtgaa aggtcgtttt gatatgatca tctccaaccc gccgttccac gatgggatgc
    31801 aaaccagcct ggatgccgcg caaacgctga ttcgcggcgc ggtgcgtcat cttaatagcg
    31861 gcggcgagct gcgaattgta gcgaacgcct tcctgcctta tccggacgtg ctggatgaga
    31921 catttggctt ccacgaagtg atcgcgcaaa ccgggcgctt caaggtgtat cgcgccatta
    31981 tgacccgcca ggcgaagaaa ggttgagtat ctgatatatg tcgccgcata ccgtgtcgga
    32041 tgcggcgtac acgccttatc tgaccgacaa acgaattatc cacaatccgc tgatggacat
    32101 ttttccagca attacacctc tgtcgataat taactattga cgaaaagctg aaaaccacta
    32161 gaatgcgcct ccgtggtagc aattcttttt aagaattgac ggtatgcgaa ggtggcggaa
    32221 ttggtagacg cgctagcttc aggtgttagt gtccttacgg acgtgggggt tcaagtcccc
    32281 ccc
//
LOCUS       DAZGQL010000060        32249 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.060, whole genome shotgun sequence.
ACCESSION   DAZGQL010000060 DAZGQL010000000
VERSION     DAZGQL010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32249
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.060"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(38..421)
                     /locus_tag="ACHKIR_003032"
     CDS             complement(38..421)
                     /locus_tag="ACHKIR_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFO9990736.1"
                     /translation="MYAKSFIAFDGNGRLTGARTAQTAPYDRYTCHLCGSALRYHPQY
                     DTERPWFEHTDDGLTEHGQQCPYVRPERREIQLIKRLQQFVPDALPVVRKASWHCRQC
                     HHDYYGEQYCTHCQTGRFSEEGGAE"
     gene            complement(792..1385)
                     /locus_tag="ACHKIR_003033"
     CDS             complement(792..1385)
                     /locus_tag="ACHKIR_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFO9990737.1"
                     /translation="MYNANPNYEMNFAILKDVNEHMEGLFQRFSKLLPFRIDFAYRKD
                     TPSFGHSCKHSMCMEIYRLLSETQTMLAGYYWVMEYTPDKGLHIHFIGYLDGQRHKNS
                     YQISRQLGDIWRRITEGEGYFHLCRAKDKYPVRIDHVIHYSDKSAVDDLRYALSYLAK
                     QDQKEHGIILRRSRLPEKSNRGRPRLDSTLSEICSLV"
     gene            complement(2302..3735)
                     /locus_tag="ACHKIR_003034"
     CDS             complement(2302..3735)
                     /locus_tag="ACHKIR_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfjI family protein"
                     /protein_id="HFO9990738.1"
                     /translation="MCLLAPENPYPIYALPPLVRNAIIETQQNTQAPLAMVATSALTA
                     MSIACQNQIDVCRPGNLRGPVNIYSLILADSGERKTTVDKVFMKAFYLRDEALAEEYA
                     KLVENYSTEKEIWEQKQKALESKFHKEIRAGKDYKATESELETHLNNSPVPPQIRRTI
                     FNDTTIEGMLKYYSDSNRSFALVSSEGGVIFDSRAMSKLGIINTLWDGGSLFIDRKSS
                     PGINLKEPRLTMSAMIQPDVYHKGFCTRKKELVKTSGHHARFLMCQPTSMQGTRIITG
                     DNYSSQYQELFDKRINELIDESLAMSGERRCLHFSPQAARIWTDYYNDVESKLGGLGP
                     LRHCREYAAKNAEYMARLAGLIYHSSGEEGEISPYTAEMARELAIWYGNEYMRLSNPL
                     TFDNTALTVPVRLIPEELELFNWIKSYCIEKGILCMKKNDILQRGPNRFRKKDKINWL
                     LDLLYEQNRVVPVIEGKTLCVAPNFDL"
     gene            complement(3831..4058)
                     /locus_tag="ACHKIR_003035"
     CDS             complement(3831..4058)
                     /locus_tag="ACHKIR_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000270113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO9990739.1"
                     /translation="MWVPLTDEQRSVILKAYGTEGDRLVREKERKEITSISRSQAWKL
                     EREGNYPPRKTIGKKSCGWLLSDLLWWIQTR"
     gene            4788..6401
                     /locus_tag="ACHKIR_003036"
     CDS             4788..6401
                     /locus_tag="ACHKIR_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9990740.1"
                     /translation="MVNQTMQQNQYYRILSGESRPFPGIYEAVSEQLLLLIFKARQLY
                     QDPEYRPWFEEQHKVLLALRDNTELQKIFNKQRKENFALGASTSSQPTALTNALNVMC
                     HLYNGFDEGNISLKSWGAMQEVQTTPDFFSLGPRTWKTPELAKFVGDDLFNILTECKA
                     WSSPIPAEARQGYCHQDLSVAHSCTSELLAHLKQYHEIDESTILAITIGLAVNEFYQY
                     KQNIPEWLSCFENKNKWPWDCRKSDTEDVHRLRERLSIIGCTEFVKNMFSVASQHEIK
                     TMYNIYVHIYAAEICGWVKTSMAYFKPTDFIQYTWKFATVTPDKDEKTENTTAWERDY
                     CQITIQRDELLRLIRTNTLQDAAIHLSGCLQYAYLESVKNDIDYPGIAPNAEEKKMKY
                     TDSIEFSGIRNNIINAVKRLESFEKWYETNKVLLVSNKTIRTEKIDVVVRLAGLKAYD
                     LQEGVPDGLKWKVKDGINERIKTDTSLCLPQDISDTSLNRYRHAVTRIVNDEIDTLLM
                     EQKKKNKRFPYSKDRHAIQPLWSKCVIEQ"
     gene            complement(6494..7090)
                     /locus_tag="ACHKIR_003037"
     CDS             complement(6494..7090)
                     /locus_tag="ACHKIR_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9990741.1"
                     /translation="MAVIPMSYSPATVARRFSILDGVTIQGVLYQIIWDPKTPFAAVI
                     EAAPSVIDGDIRHKVVATLELQRRSQLEGVFVRKFWEEQDVAQIEGIVVDGAVRDVGL
                     ATFVYETVATKAGVILLSDNEQYEGGKALWQHIARRSTNLKVFILDTDSARYYPFDGD
                     RICYDGESIPESEIWSEHPDRNKHGVVLVAESVNGKAA"
     gene            complement(7220..8536)
                     /locus_tag="ACHKIR_003038"
     CDS             complement(7220..8536)
                     /locus_tag="ACHKIR_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO9990742.1"
                     /translation="MAVNLTETAIRALKAKKTSFYVWSNSSQRGTGRLGVKVQSSGSK
                     IFYFRYYVEKGTKERFIQLGIWPEMKLATANELAKKYGAWLIEGKDPQTELERQQLAE
                     QQRRQLHQSQGSFEALIHGYVNKMKIDNKRTWQDVLKRLENECYTVIPRETKAKDVTS
                     GQIKHILAGIIQRGAVVHSNRIRSYLMAAFNYGLKADNDPMNTSVGISFGLEANPVSV
                     IPKQSSAEKVGDTWLTLEELRFLMEHFAEATNVGLLMQHLIRLCIYTGGQRPFEMIAS
                     QWCDVDFQQKTLLVTADVSKNKREHLIPLTESALQELSCVQELTKEKSSPYIFPLSTN
                     GKRPVRTDSLARAIMYFRACNPDFKIFTARDLRRTCKTLMGEAGISKEIRDRIQNHAL
                     NDVSSKHYDRYDYLPEKRRALEIWEDRVNNYQQQTGNNVVNLFGRR"
     gene            complement(8833..9597)
                     /gene="nfeF"
                     /locus_tag="ACHKIR_003039"
                     /gene_synonym="yqjH"
     CDS             complement(8833..9597)
                     /gene="nfeF"
                     /locus_tag="ACHKIR_003039"
                     /gene_synonym="yqjH"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase NfeF"
                     /protein_id="HFO9990743.1"
                     /translation="MNNTPRYPQRVRNDLRFRELTVLRVERISVGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQSDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHEGGVASSWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            9886..10509
                     /gene="nfeR"
                     /locus_tag="ACHKIR_003040"
                     /gene_synonym="yqjI"
     CDS             9886..10509
                     /gene="nfeR"
                     /locus_tag="ACHKIR_003040"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HFO9990744.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDITDAQIKKIIAVIDRAAFDITQLD"
     gene            complement(10613..12133)
                     /gene="aer"
                     /locus_tag="ACHKIR_003041"
     CDS             complement(10613..12133)
                     /gene="aer"
                     /locus_tag="ACHKIR_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HFO9990745.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGYT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLTAMLWFVAAPVVTYILCALVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            12551..13930
                     /gene="ygjG"
                     /locus_tag="ACHKIR_003042"
     CDS             12551..13930
                     /gene="ygjG"
                     /locus_tag="ACHKIR_003042"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0033094 - putrescine--2-oxoglutarate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HFO9990746.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCEQVIKAARKALA
                     AMRVSVEEA"
     gene            complement(13972..14304)
                     /gene="ygjH"
                     /locus_tag="ACHKIR_003043"
     CDS             complement(13972..14304)
                     /gene="ygjH"
                     /locus_tag="ACHKIR_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HFO9990747.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRIV"
     gene            14523..15506
                     /gene="ebgR"
                     /locus_tag="ACHKIR_003044"
     CDS             14523..15506
                     /gene="ebgR"
                     /locus_tag="ACHKIR_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HFO9990748.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTSALRAAACALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDLPGKADIREVAFAEYGRLKHVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            15690..18782
                     /gene="ebgA"
                     /locus_tag="ACHKIR_003045"
     CDS             15690..18782
                     /gene="ebgA"
                     /locus_tag="ACHKIR_003045"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HFO9990749.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLIGKQLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGEHVVHSSTIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDADGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDNGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHALDLTRGELKVENNLWFTT
                     LDDYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSR
                     TRYSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKT
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQ
                     SDGEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWRYTQENIHAAQHCNELQRSDDITLNLDHQLLGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ
                     "
     gene            18779..19228
                     /gene="ebgC"
                     /locus_tag="ACHKIR_003046"
     CDS             18779..19228
                     /gene="ebgC"
                     /locus_tag="ACHKIR_003046"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HFO9990750.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVIYDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            19291..20724
                     /gene="ygjI"
                     /locus_tag="ACHKIR_003047"
     CDS             19291..20724
                     /gene="ygjI"
                     /locus_tag="ACHKIR_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO9990751.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            20858..21928
                     /gene="ygjJ"
                     /locus_tag="ACHKIR_003048"
     CDS             20858..21928
                     /gene="ygjJ"
                     /locus_tag="ACHKIR_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
                     /protein_id="HFO9990752.1"
                     /translation="MKLITAPCRALLALPFCYAFSVTADEARPAEHDDTKTPAMTSTS
                     SPSFRFYGELGVGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNAWVPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWWGDFTPDLGY
                     VIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYDDKYTYPAMM
                     NGYIQYDLAEGITWMNGLEITDGTGQLYLTGLLTPNFAARAWHHTGRADGLDVSGSES
                     GMMVSAMYEALKGIYLSTAYTYAKHRPDHADDETTSFMQFGIWYEYGGGRFATAFDSR
                     FYMKNASHDPSDQLFLMQYFYW"
     gene            21945..24296
                     /gene="ygjK"
                     /locus_tag="ACHKIR_003049"
     CDS             21945..24296
                     /gene="ygjK"
                     /locus_tag="ACHKIR_003049"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFO9990753.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVDFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKIISNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSATLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVKYNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDRVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNKRNTKPSLAAWSVMEVYNVTQDKTWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGNKAETLSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            24467..26485
                     /gene="fadH"
                     /locus_tag="ACHKIR_003050"
     CDS             24467..26485
                     /gene="fadH"
                     /locus_tag="ACHKIR_003050"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HFO9990754.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELSHEEILQLIDNFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRAVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVGLAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KSHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQSFDETILASGIVPRIPPIDGIDHPKVLSYLDVLRDKAPVGKKVAIIGCGGIGF
                     DTAMFLSQPGESTSQNIAEFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQQLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(26530..26946)
                     /gene="higA"
                     /locus_tag="ACHKIR_003051"
     CDS             complement(26530..26946)
                     /gene="higA"
                     /locus_tag="ACHKIR_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HFO9990755.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYAQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(26943..27026)
                     /locus_tag="ACHKIR_003052"
                     /pseudo
     CDS             complement(26943..27026)
                     /locus_tag="ACHKIR_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
     gene            complement(27217..28353)
                     /gene="rlmG"
                     /locus_tag="ACHKIR_003053"
     CDS             complement(27217..28353)
                     /gene="rlmG"
                     /locus_tag="ACHKIR_003053"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HFO9990756.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTPDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRCEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            28438..28941
                     /gene="ygjP"
                     /locus_tag="ACHKIR_003054"
     CDS             28438..28941
                     /gene="ygjP"
                     /locus_tag="ACHKIR_003054"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HFO9990757.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPITKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            29018..29701
                     /gene="ygjQ"
                     /locus_tag="ACHKIR_003055"
     CDS             29018..29701
                     /gene="ygjQ"
                     /locus_tag="ACHKIR_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HFO9990758.1"
                     /translation="MQARLLLRICFSRRTLKIGCLLLLIAGATIFIADRVMVNTSKQL
                     TWSDVSAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKNYDE
                     ASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRAIWL
                     AKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVGAVIDAKILHRQPKYLGPSVMIGPF
                     SEHGCPAQK"
     gene            29780..30766
                     /gene="ygjR"
                     /locus_tag="ACHKIR_003056"
     CDS             29780..30766
                     /gene="ygjR"
                     /locus_tag="ACHKIR_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFO9990759.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTAYLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKNVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLASEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFAELVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            31049..32014
                     /gene="alx"
                     /locus_tag="ACHKIR_003057"
     CDS             31049..32014
                     /gene="alx"
                     /locus_tag="ACHKIR_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HFO9990760.1"
                     /translation="MNTVGTPLLWGGFAVVVTIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
BASE COUNT         8202 a         8138 c         7929 g         7980 t
ORIGIN      
        1 tcacccggcg tgatccccag ccccgttttt ataccgttca ttctgctccc ccctcctcac
       61 tgaagcgccc ggtctggcag tgtgtgcagt actgctcccc ataataatcg tggtgacatt
      121 gcctgcagtg ccagctggct ttacgtacta cgggtaaggc atccgggacg aattgctgca
      181 gacgtttaat cagttgtatc tccctgcgtt ccggtctgac ataagggcac tgctgaccgt
      241 gctccgtcag cccgtcgtca gtgtgttcaa accagggacg ctcagtgtcg tattgcgggt
      301 ggtatctgag cgcactgccg caaaggtggc aagtgtagcg atcgtaaggt gcagtctgtg
      361 cggtacgggc acccgtcaga cgcccgttgc catcaaatgc aataaaggat tttgcgtaca
      421 tcgtcatatt cctcagccgc cagacgacac gcaggcaaca gctgtcccgg tacgggcaga
      481 gtggtcaggg tgtgaatggc ggttcagtta aaatctggac tggagtttgg gtaaaacagg
      541 ctggttgctg ttaccgcact gtggttgtca cacaatggcc cacctgcgta aaaaactgtc
      601 agcaggcagg gatggacatc gggggatgtg ctgtatcggt tgccggaagg aatggtaaag
      661 aatgtgccat ggttaaatct ccgtatggtt catacggaat agccacatcg taaaaatgcg
      721 cagtgtcgct gaaatggcct ccggaaccga ctgtaacaat tagcgccagc ccgcagttgt
      781 cgttcagggg cttacaccag agaacatatc tccgacaggg tactgtccag tcgcggtcgg
      841 ccccggttac ttttttcggg aagcctgcta cgcctcagaa taataccgtg ttctttctga
      901 tcctgtttag ccaggtagct gagggcgtaa cgtaagtcgt ctaccgcaga tttatcacta
      961 tagtgaatca cgtgatcgat acgcaccggg tatttgtctt tagcccggca cagatgaaaa
     1021 taaccctccc cttccgtgat ccgcctccag atatccccca gttgcctgga tatctgataa
     1081 gagttcttgt gacgttgccc atccagatag ccgataaaat gaatatgaag ccctttgtct
     1141 ggtgtatatt ccattaccca gtaatatccc gccagcatcg tttgggtttc gctaagtaaa
     1201 cggtatattt ccatacacat actgtgctta caggaatgac caaaactggg cgtgtctttc
     1261 ctgtaggcaa aatcaattct gaaaggtaac agtttagaaa aacgttgaaa cagaccttcc
     1321 atgtgttcat tcacatcttt cagaatcgcg aaattcattt cgtaattggg gttagcatta
     1381 tacattttat agtccttact ttataaaact cacaggaaga ggaaattatg taaatgctga
     1441 gggtacagaa acgtcgtacc acggcattaa taaaagagat aaccagcagt acagacaacc
     1501 tgttaaaact gttttgtatt acctcattac agcaatgagg taatacttca gaatacagtg
     1561 agactcactt aaactgagga ccagtacgaa ttacaatggt aaaaacacac ctcatagaat
     1621 taagtttaag gaagtaccat tcaatcactt acaggaagta atctgacatt cacacaagtc
     1681 agtatcgggt agctacaggc aacaccgcta ttgatacccc acacaccata agaagattat
     1741 ttcatacaga aaagattata tttacccata cttataaact gtaacgctac tataacgtac
     1801 gaaccaaaga taatatatcc ctaaagtgtt ctggtacgta actagtgtta aatacgaacc
     1861 agacacacac tcaatggatt aactttctca atattacgct atgagtgagt gacataattt
     1921 acacataact aatccccata caccaccaca ctgtagcata tggggataga cgcattacgc
     1981 acatacagaa tactcagcat aaagaggagt gacattacga tagaaagaga caacataaca
     2041 gataacataa ctcccattaa taatactaat taacctaaat tatttattga atacgaaaac
     2101 aatgaagtgc cctccttatc taaggccaaa gccagcaata tcctgtacaa cactctatcc
     2161 aatagtaaat agtagttaca ccagttatat ttactgactg acctgtacca aagttctgta
     2221 atacaacact gacatggtca tggcattaat ctcctccgca agtaacggag gagactatat
     2281 gttactcaac gattaagtcg ttcagaggtc aaagttaggt gcgacacaca acgtttttcc
     2341 ctcaataacc ggtacaactc tgttttgttc atacagtaaa tccagtaacc agttgatttt
     2401 atcctttttc cggaaacggt tcggaccacg ctgtaaaatg tcattttttt tcatacagag
     2461 gatccccttc tcaatgcaat agctttttat ccagttgaaa agttcaagct cctctggaat
     2521 gagtcgcaca ggtacggtca gagcagtgtt gtcaaaagtt aacggattag acaaccgcat
     2581 atactcatta ccgtaccata ttgctaattc tctcgccatt tctgcagtgt aaggggaaat
     2641 ctccccctct tcaccgctcg aatggtaaat aagtcctgcc agtcttgcca tatactctgc
     2701 atttttggca gcatattccc gacaatgtct taaaggcccc aatcccccca gtttcgattc
     2761 cacatcattg taataatccg tccagattct ggcagcctga ggagaaaagt gaaggcaacg
     2821 tcgttcacca ctcatcgcca gactttcatc aataagttca ttgatcctct tatcaaataa
     2881 ctcctgatac tgtgatgaat aattatctcc ggttattatc cttgtcccct gcattgatgt
     2941 tggttgacac atcaaaaacc ttgcatgatg tcctgacgtt ttcacaagtt ctttttttcg
     3001 cgtacaaaaa cctttgtggt aaacatcagg ctgaatcatc gccgacatcg tcagtcttgg
     3061 ctccttcaaa ttaattccgg gagatgattt cctgtcgatg aaaagagaac ctccatccca
     3121 caaagtgtta ataattccca gtttactcat ggcccggctg tcaaaaatta ctcccccttc
     3181 actggataca agagcaaaag aacgattgct atcggagtaa tattttaaca ttccctctat
     3241 cgttgtatca ttaaaaattg ttcgacgtat ctgcggcgga acaggagagt tattcagatg
     3301 cgtttcaagc tctgattctg ttgccttgta atctttaccg gcacgaatct ctttatgaaa
     3361 ttttgattcc agcgcttttt gtttttgctc ccatatttcc ttttctgtac tgtaattctc
     3421 aaccagtttc gcgtattctt ccgccagggc ttcatccctg agataaaatg ctttcataaa
     3481 cactttatcc acggtcgttt tcctttcacc ggaatcagcc agaatcagag agtaaatatt
     3541 aacgggccca cgtaaatttc caggtctgca cacgtcaatc tgattctgac aggcaattga
     3601 catcgctgtt aatgcggatg tcgccaccat agccaaagga gcctgtgtat tttgttgagt
     3661 ttcaattatt gcatttctca ccagcggtgg tagtgcatat atcggataag gattttctgg
     3721 tgctagtaag cacataaagc ctccattttc attaatgggt tagtaaagtt gcagcaattt
     3781 ccgctacaat agcgaaaata gctgctattc agactgaaaa tacaaacagt tcatctggtc
     3841 tgaatccacc agagaaggtc acttagtaac cagccacaag atttctttcc gatagtctta
     3901 cgcggtgggt agtttccctc tcgttcaagt ttccaggctt gtgaacggga aattgaggtg
     3961 atctcttttc gttccttttc acgtaccagt ctgtcaccct cagtcccata ggctttcaaa
     4021 attactgaac gttgctcatc tgttaatgga acccacatga cggccctcct gatatatcaa
     4081 tgattcttat ctcagttgtg agcatgaaat gactgaattt cctggacaat ccatagtgga
     4141 gattccgttg tcatattgct cacagaacac ccatggacag caaggtacat acgaaaacaa
     4201 atcaagtcaa gttaagacaa ataaaacaat acttgactca caagcccact cataggtcaa
     4261 tatgtgttga ttagcaacaa aacaaccaat aatgcacatt acaaacaatg attttaagcc
     4321 aagaagtggt tttcactgtt gacaatgaca caaaatgaat ctagtatcga cttcatgtta
     4381 tgctcagata aacttctgac agtgatagta aagaagagaa ggagggggtt aaatttgtga
     4441 ccaagatcac aaaaataaat tagtttttac tattcacaat aggagggaaa gagagccagg
     4501 cattttacct gtcattgaaa tgacaacaac agcttcaaca gatcggaata ttcaggagga
     4561 ggaagaagaa ggagatctga cgggtgttat aattgttaca acacgctgga ggaaaattat
     4621 cgtaataata gatgcattct caggtatacc ctgtatcacc ccgaaaacac ggcaggcatc
     4681 cgttttatta gtagatgcag actcagtatc aacgtatcct ttcaggagat aacatcgatt
     4741 ttaactgcca gtatccggta tgcggcaaaa ggaaaccttc ttacataatg gtaaatcaga
     4801 ctatgcaaca aaatcagtac tatcgtattt tatccggaga atcccgccca ttccctggca
     4861 tatatgaggc tgtttccgaa caactccttt tgctcatttt taaggcacgt cagctatatc
     4921 aggatccaga atatcgcccc tggtttgaag aacagcacaa agttctcttg gcattgcgtg
     4981 ataatacaga gctccagaaa atttttaaca aacaaagaaa agaaaatttt gctcttggcg
     5041 catctacatc gtctcaaccc actgctctta ctaatgcgct taatgttatg tgccatttat
     5101 acaatggctt tgatgaagga aatatcagcc tgaaaagctg gggagcgatg caagaggtac
     5161 agacaacacc agactttttc agcctgggac caagaacctg gaaaacacct gaactggcaa
     5221 agtttgtagg agatgattta tttaatattc tgactgaatg taaagcctgg agttcaccaa
     5281 ttccagcaga agcccggcag gggtattgcc atcaggatct gtctgttgcg cactcctgca
     5341 cctcagagtt gctggcacac ctgaagcagt atcatgaaat agatgaatca acaatacttg
     5401 ccataaccat tggtctggcg gtcaacgaat tctaccagta taagcaaaat attccagaat
     5461 ggctttcctg ttttgagaat aagaacaaat ggccctggga ctgcaggaag tcagatactg
     5521 aagatgtgca cagactgaga gaacgactat cgataatcgg atgtacagaa ttcgtaaaaa
     5581 atatgttctc agtcgccagt cagcatgaaa taaagacaat gtacaatatt tatgtccata
     5641 tttatgcagc tgaaatctgc ggttgggtaa aaacaagcat ggcatatttt aaaccaacag
     5701 atttcatcca gtacacatgg aaattcgcaa cagttactcc ggataaagat gaaaaaaccg
     5761 agaatacgac cgcatgggaa agggattatt gtcagataac catacaacga gatgaactcc
     5821 ttagattgat tcgcactaat actctgcagg atgcagcaat tcatctttca ggctgcctgc
     5881 aatatgcata tctggagtct gtaaagaatg atattgacta tcctggaata gcccctaatg
     5941 ctgaagaaaa gaaaatgaaa tacacagatt ctattgaatt ttctggaatt aggaataaca
     6001 tcataaatgc agtaaaacgg ctggaatcat ttgaaaaatg gtatgaaact aataaagttt
     6061 tgttggtaag taataaaaca atacgaacag agaagattga cgttgttgta cgacttgcgg
     6121 gactgaaagc ctatgatttg caggaagggg ttcctgatgg tctgaaatgg aaagttaaag
     6181 atggcataaa tgaacgcatc aaaaccgata ccagtttatg cctgcctcag gatatcagtg
     6241 atacatcgct aaaccgttat cgtcacgctg tgacgcgcat tgtcaacgac gaaatcgaca
     6301 cattactgat ggaacagaag aaaaaaaata aaagattccc ctacagcaaa gatcgacatg
     6361 cgatccaacc tttatggagc aaatgcgtga tcgagcagta gagttctgag gggataaccg
     6421 agctgttatc ccccgtttat tctgccaact ctcaggtctg tcagagttcc aagaatacga
     6481 tgcggctggc aaattatgcg gcttttccat taacagattc ggccacaaga acaacaccgt
     6541 gtttatttcg atcaggatgt tcactccaga tttctgattc aggaatactt tccccgtcat
     6601 agcagatccg atcgccatca aacggataat accgtgcgga atcagtatct aaaataaaca
     6661 ctttcagatt cgttgaacga cgcgctatat gctgccagag ggctttacct ccttcatatt
     6721 gctcgttatc acttaatagt ataacgcccg cttttgtggc taccgtttca tatacaaacg
     6781 tcgcaaggcc aacatcacgt acagccccat caacaacaat cccttcaatc tgagcgacgt
     6841 cctgctcttc ccagaatttc cggacaaaca ccccttcaag ctgtgagcga cgctgaagct
     6901 ccagagtggc aacaacttta tggcgaatat caccatcaat aacagaaggt gcagcttcga
     6961 ttaccgcagc gaacggagtc tttggatccc agatgatctg gtaaagcacg ccctgaatag
     7021 ttactccatc cagtatcgaa aaacgacgag ctacagtagc aggggagtaa gacattggaa
     7081 ttacagccat ttttgaatcc cctctgagta atacaggcac gaaaatttac ctcaataatt
     7141 atagtaaaca atacaagaac taggtgcatc aacaataccc atcaattgta cgcaattcgt
     7201 acagtaacca ggaactccct caccttctgc caaacagatt gacaacatta ttgcctgttt
     7261 gctgttgata gttattaacc cgatcctccc agatctcaag tgctctgcgt ttttctggca
     7321 aataatcgta tcgatcataa tgttttgaac tgacatcgtt caacgcatga ttttgaatac
     7381 gatcgcggat ttccttactg attccagctt ctcccattaa cgttttacag gtccgacgta
     7441 agtcacgtgc tgtaaaaatt ttaaagtcag gattacaagc ccggaaatac atgatagcgc
     7501 gagccagact gtccgtacga accggacgct taccattagt cgaaagcggg aaaatgtaag
     7561 ggctcgattt ttcttttgtt aactcctgca cacaggataa ttcctgtaat gctgattcag
     7621 tcaacggtat caaatgctct cgtttatttt tagagacatc cgcagtcacc agcagcgttt
     7681 tttgctgaaa atcaacatca caccactgac tggcgatcat ttcaaaggga cgttgccctc
     7741 cggtatagat acataatcga atcaaatgtt gcatcaataa tccaacgttg gttgcttcag
     7801 caaaatgttc cattagaaaa cgcaactctt cgagtgtcag ccaggtgtca ccgacttttt
     7861 ctgcagatga ctgtttcggt atcactgata caggattggc ctccagccca aaactaatcc
     7921 ctacacttgt gttcatcgga tcgttatcag ctttcagacc atagttaaat gcagccatca
     7981 ggtaagaacg gatccggtta gagtgaacca cagcaccgcg ctgaatgatc ccggccaaga
     8041 tatgtttaat ctgtccggaa gtcacatctt tagctttagt ctcacggggg atcactgtat
     8101 agcattcatt ttcaaggcgt ttcagaacat cctgccatgt tctcttatta tcaattttca
     8161 ttttgttaac ataaccatga atcaatgctt caaatgaacc ctgtgattga tggagttgtc
     8221 tccgctgctg ttctgcaagc tgttgtcgct ccagttctgt ctggggatcc tttccttcaa
     8281 tgagccaggc accatacttt tttgccagtt cgttcgctgt ggccagtttc atctcaggcc
     8341 atatacccaa ttgaatgaat ctttcttttg tccctttttc cacgtaatag cggaaataga
     8401 aaattttgct gccagaagac tgaactttga cgccaagtcg tccagtgcca cgttgagagc
     8461 tgttgctcca gacatagaac gatgtctttt ttgcttttaa tgctcgaata gctgtttcag
     8521 ttagattgac ggccatagaa cctcttttct aaatcagtaa caatcaaaat aactcgtaaa
     8581 tttgggtcag gtaatgggtc aggtaacgat cgtacaatgg agaaaaaacg gaaccaagcg
     8641 gaaacagaag aaattacata attagttgat tataaacaat taataaaaca attacgaaca
     8701 aaccaaaaca atacagaatg gacttgcctc atgactcata atcgcttggt cgctggttca
     8761 agtccagcag gggccaccag atatagcaaa ggctgacgag aaatcgtcag cctttttctt
     8821 tttatatatc agttactttg cgtgccagta agccgctgca cgtacccgct gtgggtcata
     8881 ctgttccgct tcaaagcggc ggcttaaatt cttaacgact ttgccttcgc cggttatcca
     8941 gatgaagtaa tcatcggcag ggatttgcat ctgcgccaga cgcgcatcta ccgcctgctc
     9001 atcatgcgcc agccattcga tattaaaacc atcaaggtgc gcgagataat cctgacaggc
     9061 gttatcccgc acgctaacca gcgcactaac ttgcggttta acggcaagtt tgctcaacgt
     9121 ttccaggcgg cggcgcagtg cgggcattcc ggattcatcg cagacataca gctgatacgc
     9181 gtaatcctct ggcaccacca gcgaaccgcg cggacctgcc accgtgagtt tatcgcccgg
     9241 ttgcgcctgc attgcccagc tgctggcgac gccaccttcg tgaataaaga aatcaatcgc
     9301 cagttcatgg cgtagttcgt catacagcgg cgtatagtca cgcgacggtg ggcgtggtcc
     9361 ttccggccag acgatgccct cttccgttac cgttggcggc acaaagtgag catcagattg
     9421 aggaaagaag agtttgctgt gatcgtcaaa gccgcgcgat gtaaaaccgt ccagcgcctc
     9481 gccgccaagg acaatgcgct gaaaaccgac gctaatacgc tcaacgcgta gcacagtcaa
     9541 ttcacggaag cgcagatcat tgcgaacgcg ctgtgggtag cggggggtgt tattcattgt
     9601 tatcgccttc atgatggtaa tcagatatat ctaaataaaa tttcgcaaat gataatgatt
     9661 gttaatcatg ataaatgcaa gcgatttgta gaactgatat gtctatagtc tgataagacg
     9721 aaccgcctct tctcaggcat cattactcaa cgccggatgc ggcgtgaacg ccttatccgg
     9781 cctacgtgtg agatgagtcc acgtttaaaa atcattattg tacttgcagt aatgctcata
     9841 tcagatataa attagatata tctaattaaa caaaaggagg ctgatatgag ccatcatcac
     9901 gaagggtgct gtaaacatga aggccagcca cgccatgagg gctgctgcaa aggtgagaag
     9961 tcagaacacg agcactgcgg acacggtcac cagcatgaac acggtcaatg ctgcggtggt
    10021 cgccacggtc gcggcggcgg tcgtcgacaa cgtttctttg gtcacggtga attgcgtctg
    10081 gtgattctgg atattctctc gcgcgatgac agccacggtt acgagttgat taaagcgatt
    10141 gaaaatctga cccaggggaa ttacacccca agcccgggcg tcatctaccc aacgctggat
    10201 tttctgcagg agcagtcgct gattaccatt cgcgaagagg aaggtggtaa gaagcagatt
    10261 gcgctgaccg aacaaggcgc gcagtggctg gaagaaaacc gcgaacaggt agagatgatt
    10321 gaagaacgca tcaaagcgcg ttgcgttggc gctgcgctgc gccagaaccc gcaaatgaag
    10381 cgggcgctgg ataattttaa agcggtactg gatttacgcg tcaaccagag cgatatcact
    10441 gatgcacaaa taaaaaagat cattgcggtg atcgaccgcg ccgcttttga tattacgcaa
    10501 ctggattaat caccgattgc cggatgtggc gtgccctacc cccgtaggcc tgataagatg
    10561 cgccagcatc gcatcaggca ttgtgcgcca accgccggat gcggcatatc gattaatgca
    10621 gcaccgtcac cgcgtcttcc agtcggctgg cgcggtgttt caccatcgcc gacacctgcg
    10681 cactctcttc cactaactcg gcgtttttct gggtgatcag gttaagctca tccactgcac
    10741 gggtcaggct ggaaagccca tcggcctgtt ccagcgttga atggctaatc tgagctatca
    10801 actgggtgac gttttttacc tgtgccacaa tatcttccat cgtccgtccg gcggcgtgta
    10861 cctgctgcga accggattgc accttatcag cactggcatc aatcagcttg cgaatatcgt
    10921 tggcagcatt ggcgctgcgg ctagctaaat gacgcacttc ccccgccacc actgcaaaac
    10981 ctttgccctg ttcgccggca cgcgccgctt ccaccgccgc attcagggcc agaatattgg
    11041 tctgaaacgc aatatcgtta atcagcgaag taatggtgcc aatgcgctgg gtactgtcgg
    11101 caatatcgtc cattgtcttg atcaccgtag tcatcgcctc cccaccctgc actgccgcat
    11161 tactggcagt gatagaaagt ttatcggcag ccgacgccgt ggcagagttc tgtttcaccg
    11221 acgccgccat ttggttcatg gtcgccaccg tttgctgaac gttatcaact gtctgctggg
    11281 tatgttcgtt cagttcatcg gtgcctttcg ccagcgtctc actgccgttt ctgacactgg
    11341 acacctggct tgagacatcg ttaattagcc aacggcacat caggccaagc tgccctaccg
    11401 cgcgtaatgt cagccccagc tcatcgctgc gattcagatg ctcaacacta ttacgctctc
    11461 cggtcgccac cttcagtgcc tgacgggcga cattttctat cgggcggaca atctgccatt
    11521 caaaacaagc gcttgccaat aataccacta acgcacagag gatatacgtc accaccgggg
    11581 cagcaacaaa ccatagcatg gccgtcagca agataaacat cagggtcatc acgccacgcg
    11641 cccgccagcg aagcggtaat gaaggcagtt tacccagcca tcctttacgc accaccaggc
    11701 ctttatgaat acgcttactg gtacgtccgg cgttcaacgc tttgtacagc ggctccaccg
    11761 ccgcgatctc ttcatccgtc gcccgggtac gaatcgacat atagccactg atttttccct
    11821 cgcgcaccat cggtaccgca ttggcccgca cccaataatg gtcgccattt ttgcggcgat
    11881 ttttcacgat gccgctccag ggctcccctt ttttcagggt gaaccacata tccgcaaacg
    11941 ccgcttttgg catatccggg tgacgcacca tgttgtgcgg ttgaccttgt aactcttgca
    12001 aggtatagcc gctcacctgc acaaaagtgt cattagcatg agtgatatag ctttgcagat
    12061 cggtagtgga catcagagtg gtatcgtccg ccagcggggt attttgctgg gtgacatacg
    12121 gatgagaaga catgttcgcg tcctgtgcag gttatatggt tgttaacttc ttgtcagagt
    12181 ttatgtcggc cccgctgcgg ttatctttaa ctgattaatt tgatttagat cgcaatttgc
    12241 gattaaagca taaatctaat cccttgattt aaaatacttt cactctgtta ctatacgaaa
    12301 acgttaatta tcttgcccaa aaatcaggca gttgttgccc tgaaagtgtg catttgcgca
    12361 gcaatcgcca aatccatacc cgacaaaaac cgtgcaaaat aacaacagat gttaacaaat
    12421 agcattaaat attgcgcaaa tgataaccac atttgtgttt atcccgattt tcgcgatcgc
    12481 agccggagtg gcgcaatccc tgcaatactt aaatcggtat catgtgatac gcgagcctcc
    12541 ggagcatatt ttgaacaggt taccttcgag cgcatcggct ttagcgtgca gcgcccacgc
    12601 cctgaatctc attgagaagc gaacgctgga tcatgaggag atgaaagcac ttaaccggga
    12661 ggtgattgaa tacttcaaag agcatgtcaa tccggggttt ttagagtatc gcaaatctgt
    12721 taccgctggc ggggattacg gagccgtaga gtggcaagcg gggggtttaa atacgcttgt
    12781 cgacacccag ggacaggagt ttatcgactg cctgggaggt tttggaattt tcaacgtggg
    12841 gcaccgtaat ccagttgtgg tttccgccgt acagaatcaa cttgctaaac aaccgcttca
    12901 cagccaggag ctgctcgatc cgttacgggc gatgctggcg aaaacccttg cggcgctaac
    12961 gcccggtaaa ctgaaataca gcttcttctg taatagcggc accgaatccg ttgaagcggc
    13021 gctgaagctg gcgaaggctt atcagtcacc gcgcggcaag tttactttta ttgccaccag
    13081 cggcgcgttc cacggtaaat cacttggcgc gctgtcggcc acggcgaaat cgaccttccg
    13141 caaacctttt atgccactgc tgccagggtt tcgtcatgta ccgtttggca atatcgaagc
    13201 catgcgcacg gctcttaacg agtgcaaaaa aaccggtgat gatgttgctg cggtgatcct
    13261 cgaaccgatt cagggcgaag gtggcgtcat tctgccgccg ccgggatatc tcaccgccgt
    13321 gcgtaagctg tgcgatgagt tcggcgcact gatgatcctc gatgaagtgc aaaccggtat
    13381 ggggcgcacg ggcaagatgt tcgcctgcga gcatgagaac gtacagccgg acatcctgtg
    13441 tctggcaaaa gccctcggcg gcggcgtgat gccgattggc gcaaccatcg ccactgaaga
    13501 ggtgttttcc gttctgttcg acaacccgtt cctgcatacc accacctttg gcggcaaccc
    13561 gctggcctgt gcggcggcgc tggcgaccat caatgtgttg ctggagcaga acttaccggc
    13621 tcaggctgaa caaaaaggcg atatgttgct ggacggtttt cgtcaactgg cgcgggaata
    13681 tcccgatctg gtacaggaag cgcgtggtaa agggatgttg atggcgattg agtttgttga
    13741 taacgaaatc ggctataact ttgccagcga gatgttccgc cagcgcgtcc tggtggccgg
    13801 aacactcaac aacgctaaaa cgatccgcat tgaaccgcca ctgacactga ccattgaaca
    13861 gtgtgaacag gtgatcaaag cggcgcgtaa ggcgctggcg gccatgcgag tcagtgtcga
    13921 agaagcgtag tatcaaatcg gatggcgata cgacgtcgcc atccgatctt tttatacgat
    13981 acgtacgccc gcaggcatca tccgctccgg cgttaacagc acgctttcac tgccgtcatc
    14041 ggtttccgca cacaacagca tgcattccga cgtttcaccg cgcattttcg ctttttgcaa
    14101 attgcataag accaccactg tttttcccat cagctcttct tcgctgtagt acggcaccag
    14161 gctggtcacg gtttgcagcg ttttttcccc cacatcaacc tgtacgatgt acagcttgtc
    14221 ggcgttttca tggcgtttca cttccacaat ctttccgacg cgcatttcca gacgtgcaaa
    14281 atcagcgtaa gccacggttt ccattgtttc ctcccttcga gtaaaatttt actaaaccat
    14341 agcaaaagtt tttctcaatc ctgtaggcta aaaatggaga atgcaggcgt gatcacattc
    14401 gcaagccgct gtgttaccgt tacagcgtca aagaaacgcg ctttatttac tgaaaacagg
    14461 tgacccgata agcacttcct ctacaatggg ggcgcacatc agggaaagta aaaaaggtaa
    14521 acatggcaac actaaaagac atcgcaatcg aagctggcgt atccctggcg acagtatcca
    14581 gggtcttgaa tgacgatccg acattgaatg tgaaagaaga gacgaaacat cgcattctcg
    14641 agatcgccga aaagctggag tacaagacca gtagcgcccg taaactccag acaggcgcag
    14701 tcaaccaaca ccatattctg gctatctaca gctaccaaca ggagctggag atcaacgatc
    14761 cttactatct ggcgatccgc cacggcattg aaactcagtg cgaaaagctg gcgatcgaac
    14821 tgaccaactg ttatgaacac aacggcttac cagacattaa aaacgtcacc ggtattttaa
    14881 ttgtcggcaa acccacgtcc gccctgcgcg ccgccgcctg tgcgttgacc gacaatatct
    14941 gttttatcga ctttcacgaa cccggcagcg gttacgatgc ggtggatatc gatctggcac
    15001 gtatcagtaa agaaatcatc gatttctata tcaatcaggg cgttaatcgt attggtttta
    15061 ttggcggtga agacttgcct ggcaaggcgg atattcgtga ggtcgccttt gcggaatatg
    15121 gtcgtctgaa acacgtggta cgcgaagagg atatctggcg cggcggtttt tccagttcgt
    15181 cgggctatga actggcaaaa cagatgctgg cgcgggaaga ctatccgaag gcactgtttg
    15241 ttgcttccga ttccattgct atcggcgtac tgcgggcaat tcatgaacgt ggcctgaaca
    15301 tcccacagga tatttcgctt atcagcgtta acgatatccc caccgcgcga tttacctttc
    15361 cgccgctctc caccgtgcgc atccattccg agatgatggg aagtcagggc gttaacctgg
    15421 tgtatgaaaa agcccgcgat ggtcgcgcgc tgccgctgtt ggtcttcgtt cccagcaaat
    15481 taaaactgcg cggcacgacc cgttaaatcc ccttgcacgc tgtccggcaa tcgtttttgc
    15541 cggacagtgc tgccgtttat tttcgtgatc cagttaaagt aaatgcattt atccgctact
    15601 ttttagtaaa atttttacta aactccccag caattacaca aactaccatc accataaatg
    15661 gttccgattt ctctctaccg ggaggcccta tgaatcgctg ggaaaacatt cagctcaccc
    15721 acgaaaaccg acttgcgccg cgtgcgtact ttttttcata tgattctgtt gcgcaggctc
    15781 gtacttttgc ccgtgaaacc agcagcctgt ttctgccctt aagcggtcag tggaatttcc
    15841 acttttttga ccatccgctg caagtgccag aagccttcac ctctgagtta atggctgact
    15901 gggggcatat taccgtcccc gccatgtggc aaatggaagg tcacggcaaa ctgcaatata
    15961 ccgacgaagg ttttccgttc cccatcgatg tgccgtttgt ccccagcgat aacccaaccg
    16021 gtgcctatca acgcattttc accctcagcg acggctggca gggtaaacag acgctgatca
    16081 aatttgacgg tgtcgaaacc tattttgaag tctacgttaa cggtcagtat gtggggttca
    16141 gcaaaggcag tcgcctgacc gcagagtttg acatcagcgc gatggttaaa accggcgaca
    16201 acctgttgtg tgtgcgcgtg atgcagtggg cggactctac ctacgtggaa gaccaggata
    16261 tgtggtggtc ggcggggatt ttccgcgatg tttatctgat cggaaaacaa ctcacgcata
    16321 ttaacgattt caccgttcgt accgactttg acgaagccta ttgcgatgcc acactttcct
    16381 gcgaagtggt gctggaaaat ctcgccgcct cccctgtcgt cacgacgctg gaatataccc
    16441 tgtttgatgg cgaacacgtg gtgcacagca gcaccattga tcatttggca attgaaaaac
    16501 tgaccagcgc cagttttgct tttactgtcg aacagccgca gcaatggtca gcagaatccc
    16561 cttatcttta ccatctggtc atgacgctga aagacgccga cggcaacgtt ctggaagtgg
    16621 tgccacaacg agttggcttc cgtgatatca aagtgcgcga cggtctgttc tggatcaata
    16681 accgttatgt gatgctgcac ggcgtcaacc gccacgacaa cgatcatcgc aaaggccgcg
    16741 ccgttgggat ggatcgcgtc gagaaagatc tccagttgat gaagcagcac aacatcaact
    16801 ccgtgcgtac cgctcactac ccgaacgatc cacgttttta cgaactgtgt gatatctacg
    16861 gcctgtttgt gatggcggaa accgacgtcg aatcgcacgg ctttgctaat gtcggcgata
    16921 tcagccgtat taccgacgat ccgcagtggg aaaaagttta cgtcgagcgc attgttcgcc
    16981 atattcacgc gcagaaaaac catccgtcga tcatcatctg gtcgctgggc aatgaatccg
    17041 gctatggctg taacatccgc gcgatgtacc atgcggcgaa ggcgctggat gacacgcgac
    17101 tggtgcatta cgaagaagat cgcgatgctg aagtggtcga tattatttcc accatgtaca
    17161 cccgcgtgcc gctgatgaat gagtttggtg aatacccgca tccgaagccg cgcatcatct
    17221 gtgaatatgc tcatgcgatg gggaacggac cgggcgggct gacggagtac cagaacgtct
    17281 tctataagca tgattgcatt cagggacatt atgtctggga atggtgcgac cacgggatcc
    17341 aggcgcagga tgacaacggc aatgtctggt ataaattcgg cggcgactac ggcgactatc
    17401 ccaacaacta taacttctgt cttgatggtt tgatctattc cgatcagacg ccgggaccgg
    17461 gcctgaaaga gtacaaacag gttatcgcgc cagtaaaaat ccacgcgctg gatctgactc
    17521 gcggcgagtt gaaagtcgaa aataatctgt ggtttaccac gcttgatgac tacaccctgc
    17581 acgcagaggt gcgcgccgaa ggtgaaacgc tcgcgacgca acagattaaa ctgcgcgacg
    17641 ttgcgccgaa cagcgaagcc cccttacaga tcacgctgcc acagctggac gcccgcgaag
    17701 cgtttctcaa cattacggtg accaaagatt cccgcacccg ctacagcgaa gccgggcatt
    17761 ctatcgccac ttatcagttc ccgctgaagg aaaacaccgc gcagcctgta cctttcgcac
    17821 cgaataacgc ccgtccgctg acgctggaag acgatcgttt gagctgtact gttcgcggct
    17881 acaactttgc gatcaccttc tcaaaaacga gtggtaaacc gacatcctgg caggtgaatg
    17941 gcgagtcgct gctaacccgc gagccaaaaa tcaacttctt caagccaatg atcgacaacc
    18001 acaagcagga gtacgaaggg ctgtggcagc cgaatcattt gcagatcatg caggaacatc
    18061 tgcgcgactt tgccgtagaa cagagcgatg gtgaagtgtt gatcatcagc cgcacggtta
    18121 ttgccccacc ggtgtttgac ttcgggatgc gctgcaccta catctggcgc atcgctgccg
    18181 atggccaggt taacgtggcg ctttccggcg agcgttacgg cgactatccg cacatcattc
    18241 cgtgcatcgg tttcaccatg gggattaacg gcgaatacga ccaggtggcg tattacggtc
    18301 gtggaccggg cgaaaactac gccgacagcc agcaggctaa catcatcgat atctggcgca
    18361 gcaccgtcga tgccatgttc gagaactatc ccttcccaca gaacaacggc aaccgtcagc
    18421 atgtccgctg gacggcactg actaaccgcc acggcaatgg tctgctggtt gttccgcagc
    18481 gccccattaa cttcagcgcc tggcgctata cccaggaaaa catccacgct gcccagcact
    18541 gtaacgagtt gcagcgcagt gatgacatca ccctgaatct cgaccaccag ctgcttggcc
    18601 tcggctccaa ctcctggggc agcgaggtgc tggactcctg gcgcgtctgg ttccgtgact
    18661 tcagctacgg ctttacgttg ctgccggttt ctggcggaga agctaccgcg caaagcctgg
    18721 cgtcgtatga gttcggcgca gggttctttt ccacgaattt gcacagcgag aataagcaat
    18781 gaggatcatc gataacttag aacagttccg ccagatttac gcctctggca agaagtggca
    18841 acgctgcgtt gaagcgattg aaaatatcga caacattcag cctggcgtcg cccactccat
    18901 cggtgactca ttgacttacc gcgtggagac ggactccgcg accgatgcgc tatttaccgg
    18961 gcatcgacgc tattttgaag tgcattacta cctgcaaggg cagcaaaaaa ttgaatatgc
    19021 gccgaaagag acattacagg tcgtggaata ttatcgtgat gaaactgacc gtgaatattt
    19081 aaaaggctgc ggagaaaccg tagaggtcca cgaagggcaa atcgttattt acgatatcca
    19141 tgaagcatat cggtttatct gcaataacgc ggtcaaaaaa gtggttctca aagtcaccat
    19201 cgaagatggt tatttccata acaaataaca actacggcgg caaaaggagt ttgccgccac
    19261 cgctacccta ctcattttcg gagatgtgtt atgtctgata ccaaacgtaa tacaatcggc
    19321 aaattcggct tgctctcgct gacttttgcc gccgtttaca gctttaacaa cgttatcaat
    19381 aataatattg agcttggact ggcctcggca ccgatgtttt tcctcgcgac gattttttat
    19441 tttattccct tctgtctgat catcgcagaa tttgtttcgt taaataaaaa ctcagaagcc
    19501 ggtgtctacg cgtgggtaaa aagttcgctg ggcgggcgtt gggcatttat tactgcctat
    19561 acctactggt tcgtaaacct gttctttttc acctcactgt tgccgcgcgt tattgcttat
    19621 gcttcgtatg ccttcctcgg ctacgaatat attatgacgc cggttgccac caccattatc
    19681 agtatggtgc tgttcgcctt ctccacctgg gtttccacca acggggcgaa aatgctgggg
    19741 ccaattacct ccgtcacttc aacgctgatg ctgctgttaa cgctctccta cattttactg
    19801 gcaggtacgg cactggttgg cggcgtacag cctgccgatc cgatcaccgt tgacgcgatg
    19861 atcccgaact tcaactgggc gttcctcggc gttaccacct ggatctttat ggccgcaggt
    19921 ggcgcggagt ccgtcgcggt gtacgttaac gacgtcaaag gcggttcgaa atcgttcgtt
    19981 aaagtgatca tcctcgccgg gatttttatc ggcgtactgt attccgtttc ctcggtgctg
    20041 attaacgtct tcgtcagcag caaagagttg aaatttactg gcggatcggt gcaggtattc
    20101 cacggcatgg cggcgtattt tggtctgccg gaagcgttga tgaatcgctt tgtcggtctg
    20161 gtgtccttta ccgcgatgtt cggttccctg ctgatgtgga ccgcaacgcc ggtgaaaatt
    20221 ttcttctccg aaatcccgga aggcatcttt ggtaagaaaa ccgtcgaact gaacgaaaac
    20281 ggcgttccgg cgcgcgcagc gtggatccag ttcctgatcg tcatcccgct gatgattatc
    20341 ccgatgctcg gttccaatac cgtgcaagat ctgatgaata ccattattaa tatgaccgcc
    20401 gcagcgtcca tgcttccgcc gttattcatc atgctggctt acctgaattt acgcgccaaa
    20461 ttagatcacc tgccacgcga tttccgtatg ggctcccgcc gcaccggtat tatcgttgtt
    20521 tcaatgctga ttgcgatatt tgccgtaggg tttgtcgctt cgacattccc gactggcgcg
    20581 aatattctga ccatcatttt ttataacgtc ggcggtattg ttatcttcct cggctttgcg
    20641 tggtggaaat acagtaaata tataaaggga ttaacggctg aagagcgcca tattgaagcg
    20701 acgccagcca gcaatgttga ttaagcagaa taataaaaaa tgagcaggca ttttccctct
    20761 tcctggatgg ggagagggaa aatagtttct gcctttatat ttttataatt acaacgataa
    20821 aaggctgtac tttttcttta gctcatggat taacacaatg aaattaatca ctgcaccatg
    20881 cagagcatta cttgcgctgc cgttttgcta cgccttttct gtgaccgctg atgaagcacg
    20941 tccggcagaa catgacgaca caaaaacccc cgcaatgacc tcgacatctt ctccttcatt
    21001 tcgtttttac ggcgaattag gggttggtgg gtatatggat ttagagggtg agaataaaca
    21061 taaatacagc gacggcacct atattgaagg tggcctggag atgaagtacg gatcctggtt
    21121 cggcctgatt tacggcgaag gctggaccgt gcaggccgac cacgacggta atgcctgggt
    21181 gcctgaccat agctggggcg gtttcgaagg tgggattaac cgtttctatg gcggttatcg
    21241 taccaatgat ggcaccgaaa tcatgctcag tctgcgtcag gattcctcgc tggatgacct
    21301 gcaatggtgg ggcgatttca cgcccgatct gggctacgtc attcccaata cccgcgacat
    21361 tatgaccgcg ctgaaggtgc agaacttaag cggcaacttt cgttatagcg ttaccgccac
    21421 acctgccgga catcatgacg aaagcaaagc ctggctacat tttggcaagt acgatcgcta
    21481 cgacgacaaa tacacctatc cggcaatgat gaacggttac atccagtatg accttgccga
    21541 aggcatcacc tggatgaacg gtctggaaat caccgacggc acaggccaac tctatctgac
    21601 gggcctgctt acccctaact ttgccgctcg cgcctggcac cataccggac gcgccgacgg
    21661 gctggacgta tcgggaagtg aaagcgggat gatggtgagc gccatgtatg aagcgttaaa
    21721 aggcatttat ctctccaccg cttacaccta cgccaaacat cgccctgacc acgctgacga
    21781 tgaaaccacc tctttcatgc agtttggtat ctggtacgaa tacggcggcg gacgtttcgc
    21841 aacggctttt gatagccgct tctacatgaa aaatgcctct cacgatccca gcgaccaact
    21901 cttcctgatg caatatttct actggtaata aggactgtaa tttcatgaaa atcaaaacta
    21961 ttttaacgcc agtaacctgc gctctgctga taagtttttc cgcccatgcc gctaacgccg
    22021 acaattataa aaacgtgatt aaccgtactg gcgcgccgca gtacatgaag gattacgatt
    22081 acgacgatca ccagcgtttt aatccgtttt tcgatctcgg agcctggcat ggtcatctgt
    22141 tgccagacgg cccgaacacc atgggcggtt ttccaggcgt tgcgctgctg acggaagaat
    22201 acatcaactt tatggccagc aatttcgatc gtctgaccgt ctggcaagac ggcaagaaag
    22261 tcgacttcac gctggaggca tacagtattc ccggcgcgct ggtgcaaaaa ctgacagcaa
    22321 aagatgtgca ggtcgaaatg actctgcgct tcgccacgcc gcgcacgtca ctactggaaa
    22381 ctaaaatcat cagcaataaa ccgctggatc tggtgtggga cggcgagctg ctggaaaaac
    22441 tggaagcgaa agaagggaaa ccgctttccg ataaaaccat tgctggcgaa taccctgact
    22501 atcagcgcaa aatcagcgcc acccgtgatg gcctgaaagt cacctttggc aaagtgcgcg
    22561 ccacctggga tctgctgacc tccggtgaat cagaatatca ggtgcataaa tccctgccag
    22621 tgcagactga aatcaacggt aatcgcttta ccagtaaggc gcatatcaac ggttcggcca
    22681 cgctctatac cacctactcc cacctgctga ccgctcagga agttagcaaa gagcaaatgc
    22741 agatccgcga tattctggcg cgtccggcgt tttatctcac cgcctcgcag caacgctggg
    22801 aagagtatct gaagaaaggg ttaaccaatc cggatgcgac gccggaacag acgcgcgtcg
    22861 cggtgaaagc gatcgaaacg ctcaacggta actggcgctc gcctgggggg gcggtgaaat
    22921 ataacaccgt cacgccgtcg gtgaccgggc gctggttctc cggcaatcag acctggccgt
    22981 gggatacctg gaagcaggca tttgcgatgg cgcatttcaa tccggatatc gccaaagaga
    23041 atatccgcgc ggtcttctcc tggcagatcc agccaggcga ccgcgtgcgt ccgcaggatg
    23101 tgggctttgt ccccgacctg atagcatgga accttagccc cgagcgtggc ggcgatggcg
    23161 gcaactggaa caaacgtaat accaaaccca gccttgccgc ctggtcagta atggaagtgt
    23221 ataacgtcac ccaggataaa acctggctgg cagagatgta cccgaaactg gtggcctatc
    23281 acgactggtg gctacgtaac cgcgatcaca acggcaacgg cgtgccggaa tatggcgcga
    23341 cccgcgacaa agcccacaac actgagagcg gcgagatgct gtttacggtg aagaaaggca
    23401 acaaagcaga gacgctgtct gggctgaaca actacgcccg cgtggtagag aaaggccagt
    23461 acgacagtct ggaaatcccg gcacaggttg cggcgtcgtg ggaatcgggg cgtgatgatg
    23521 ccgccgtctt tgggtttatc gacaaagaac agctggataa atatgtcgct aacggcggca
    23581 aacgtagcga ctggacggtg aaattcgccg aaaaccgcag tcaggacgga acgttgctgg
    23641 gctactcgct attgcaggag tcggtggatc aggccagcta tatgtacagc gataaccatt
    23701 atctggcgga gatggcgacg attctcggta agccggaaga ggccaaacgc tatcgtcagt
    23761 tagcacaaca gctcgcggac tacatcaaca cctgtatgtt cgacccgact acccagttct
    23821 actatgacgt gcgcattgaa gataaaccac tggcgaacgg ctgcgcgggc aaaccgattg
    23881 tcgagcgcgg caaagggccg gaaggctggt cgccgctgtt taacggtgcg gcaacgcagg
    23941 ccaacgccga cgcggtggtg aaggtgatgc tcgatcctaa agagttcaac acctttgtac
    24001 cgctgggaac ggcggcgtta accaatccgg cctttggcgc agatatttac tggcgcgggc
    24061 gcgtgtgggt ggatcagttc tggtttggtc tgaaagggat ggagcgttac ggttatcgcg
    24121 atgatgccct gaagctggcg gatacgttct tccggcacgc caaaggatta accgccgatg
    24181 gcccaattca ggagaattac aacccgctga ctggcgcaca gcaaggcgca ccaaatttct
    24241 cctggagtgc cgcgcatttg tatatgttgt ataacgattt tttccgtaag cagtaatttt
    24301 tcccgatgcc ggacgactgt attaccgccg tccggcatga cgaacgggcg tattcttttt
    24361 gaatcccatc acaaaccccg cactcccctt ttcccttttc tccagcgacg gctaaattag
    24421 aactcatccg accacataac aattatttta catactggac aattttatga gctacccgtc
    24481 gctgttcgcc ccgctggatt taggcttcac cacgttaaaa aaccgagtac tgatgggttc
    24541 aatgcacacc ggactggagg aatacccgga cggcgctgaa cggctggcag cgttttacgc
    24601 ggaacgcgcc cgtcatggcg tggcgttgat tgtcagcggc ggcattgcac cagatttaac
    24661 aggcgttggc atggaaggcg gcgcaatgct caacgacgcc agccagatcc cacaccatcg
    24721 caccattacc gaagccgtac atcaggaagg cggcaaaatt gccttacaaa ttttgcatac
    24781 cgggcgctac agctaccaac cgcatctggt cgctccctcc gcattgcagg cccccatcaa
    24841 ccgtttcgtg ccccatgagt taagccatga agagatcctg caactgatcg acaatttcgc
    24901 ccgctgcgcg caactggcgc gggaggccgg atacgacggc gtagaagtga tgggttccga
    24961 agggtatttg atcaacgagt ttctgaccct gcgcaccaac cagcgtagcg accagtgggg
    25021 cggcgattac cgcaaccgga tgcgctttgc agttgaagtg gtgcgagcag tgcgcgaacg
    25081 cgtcggcaat gacttcatta ttatctaccg tctgtcgatg ctcgacctgg tagaaggcgg
    25141 cggtactttt gcagaaacgg tagggctggc gcaggccatt gaagcggcgg gcgcgaccat
    25201 tatcaacacc ggcattggct ggcatgaagc acgtattccg accattgcca cgcccgtgcc
    25261 gcgcggtgcc tttagctggg tcacgcgcaa actgaaaagc cacgtctcgc tgccgctggt
    25321 gaccaccaac cggattaacg atccgcaggt tgccgacgac attctctcgc gcggtgatgc
    25381 cgatatggta tcgatggcgc gaccgtttct tgctgatgcg gaactgctgt caaaagcgca
    25441 atcggggcga gccgatgaga tcaacacctg tattggctgc aatcaagcct gcctcgatca
    25501 gatcttcgtt ggcaaagtca cctcgtgcct ggtgaatcct cgcgcctgcc acgaaaccaa
    25561 aatgccgatc cttcccgccg tgcagaaaaa aaatctggcg gtggtcggcg ctggtccggc
    25621 tgggctggcg tttgccatta acgcggcggc acgtggtcat caggtaacgt tgtttgatgc
    25681 tcacagtgag atcggcggtc agtttaatat cgccaaacag atccctggca aagaggagtt
    25741 ttacgaaacg ctgcgctatt accgccggat gatcgaagta acaggcgtga cgctgaaact
    25801 caatcacacc gtgacggcgg atcagttaca gtcgttcgat gaaacgatcc tcgctagcgg
    25861 gatcgtaccg cgcattccac ctatcgacgg aatcgatcat ccgaaggtgt tgagttatct
    25921 cgacgtactg cgcgacaaag cgcctgtggg caaaaaggta gcaatcatcg gttgtggcgg
    25981 gattggtttt gatacggcga tgtttttaag tcagccgggt gagtccacca gccagaacat
    26041 tgccgagttc tgtaatgagt gggggatcga cagcagcctg caacaggctg gtggcttaag
    26101 cccgcaggga atgcagatcc cgcgtagccc acggcagatt gtgatgctcc agcgcaaagc
    26161 cagcaaaccg ggacaggggt taggcaaaac caccggctgg atccatcgca ccaccctgct
    26221 ctcgcggggt gtgaaaatga tcccaggcgt aagttatcag aagattgacg atgacgggct
    26281 gcatgtggtg atcaacggcg aaacgcaggt attagcagtg gacaatgtgg tgatctgcgc
    26341 agggcaagag ccaaaccgcg cgctggcgca acagctgatt gatagcggga aaactgtgca
    26401 tttaattggc ggctgcgatg tagctatgga gcttgacgca cgacgggcaa ttgcccaggg
    26461 aacacggctg gcgctggaga tttaaatcgt tgtttgaagc gccggatgcg acgcatccgg
    26521 cacgtcgcgt taatcaataa acaaggcggg agaaatgccg aatcgcgttg ccagtttttt
    26581 agcgtgttcc agcgttaatt tcctcttccc gctcaaaacg cgtgacacca tggatttact
    26641 gccaatttcc ggaagatcgg aaagggttaa accatattga tccataaggg tacgaatcac
    26701 ggctataccg ccaggcatgg cttgagccat ggcattaaat tcagcaaatt cgggcgctga
    26761 ttcttcccac gcggttattt tggcacacac cagatccagc aagggatttt caggatcgtt
    26821 gagcagcaga tgatcgacca gttccagcgc ctgggcgtat tgttcctcgt tctgaatacc
    26881 cgccagaaaa ggtgccacag cagttagctt ttctcctgct tgcaagatgt cggcaatcgc
    26941 aatcattttt tccccttagt acgatgaacg gcggtaaaga aatcgtattc tttatgcgtc
    27001 ataacttcac gtatgtagca taaacgtaga aaaacgctaa acaagagaat gtagaggatg
    27061 gtgacaggga ttttttgttt tatggaagcg cgattccaga actgggcaga cattgccgga
    27121 tgcgacgctt gccgcgtctt atccgacctg ggggtaatgt gctgaacgta ggccggataa
    27181 ggcgtttacg ccgcatccgg caggtaactc ccaatcttat cgacgacgcc ccagcttcac
    27241 tgctttcagc accacaaatt tattattcgt ggcgatggtg gtgcagttgc cgaaaatttt
    27301 cttcagttta tggaagtaat ccaggtgacg gttggcaacg atatacagct cgccgttgat
    27361 tttcaggcag cggcgggcat ggtggaacat ctcccaggcg acgttatcgg tcagcgcatg
    27421 ttgctgatga aacggcgggt tgcagagcac agcattaaaa cggaaaggct ccacgccgga
    27481 gagcgcgttg ttgatcataa actcgcagcg atccaacgcc tcgggcatgt tggtttcaac
    27541 gttcaaacgg ctggaagcaa ccgccatcgg tgattcatcg acaaacacca ctttcgcctg
    27601 cgggttttta tcgagcaatg tcaggccaat aacgccatta ccgcaaccga gatcgacaat
    27661 ctcaccttcg agattctctg gcagatgttg cataaagaag cgcgcgccaa tatccagccc
    27721 agtgcgggag aaaacattcg catggttgtg gatagtccag tcagtgcctt ccagcttcca
    27781 gctaacggtc tgcggcgcat cggccaacgg cggttcgttg aaggtgcagt taatcaggcg
    27841 cgctttcttc catgccagcg tggtggtggt cggaccgagc actttttcga acagttccag
    27901 cgtggaagtg tggatgtcac gggctttggc accggcaata atacgtgtat ccggcgtgac
    27961 cactttgcgc agcgcacgca gttgttgttc cagcaacgcc agtgttttcg gtactttgat
    28021 cagtaccacg cccggctgtt gcgggtagtc ggcggtgctg tcgagaaact tcacgctcga
    28081 ctcatcaatc ccgttgaggc gtaaattctc gcgcgtcgcc agttcgctga tgtatgagtc
    28141 gccaatgctg tatggcttat gttccgccag cgcacaactt aacgcaccaa aggcatcatt
    28201 caggatcaac accgggccgc ggatttctgt gtcgtccaac tgttgcagca aatattcatc
    28261 cgccgcttcc cacgcctgta gtgggttaac gtcatccgtc gccggaaaac gttgtagtgt
    28321 cagtgaacgg aaaccgttgt ctaagtggct catcggccct cctgaatgat aaaatttcga
    28381 cgttgcccct gaaaagggtg cgtgagtata ccctttttct ctttttcgtg ggcggttatg
    28441 agcaatctta cttatcttca gggttatccc gagcagctac tttcccaggt gcgaacgctg
    28501 attaacgaac agcgtctggg agatgtgctg gcaaaacgct atccgggaac acacgactac
    28561 gccaccgata aagccctctg gcaatatact caggatctta aaaatcagtt tctgcgcaat
    28621 gccccgccga tcactaaagt gatgtatgac aataagatcc acgtgctgaa aaacgcgctc
    28681 ggattacata ctgccgtttc gcgtgtgcag ggcggcaagc tgaaagcaaa ggcggagatc
    28741 cgcgtcgcta ccgtgtttcg caacgcgccg gaaccgtttt tgcgcatgat cgtggtgcac
    28801 gagttggcgc acctgaagga gaaagagcat aacaaagcgt tctatcaatt gtgctgccat
    28861 atggagccgc agtatcacca gcttgagttc gacacccggc tgtggctaac gcagttgtcg
    28921 cttgggcagg acaaaatctg aaaatgatgt caggcgttat cttttcttcc tatagcgcct
    28981 aaaatcgacc tccccccttt cgttaaggat aacgaccatg caggcccgcc ttcttctccg
    29041 tatttgtttt tctcgccgga ctcttaaaat tggctgttta ttgttgctta tagcaggagc
    29101 gaccattttt attgccgatc gcgttatggt caacaccagc aagcagctga cctggagcga
    29161 tgtcagcgca gttccggcgc gtaacgtggg tttattgctg ggggcgaggc cgggtaatcg
    29221 ctactttacc cgacgtattg ataccgcagc agcgttgtac cacgccggaa aagtgaaatg
    29281 gctactggta agtggcgata acgggcgtaa aaattacgat gaagcctcag gtatgcagca
    29341 ggctttgatc gctaaaggcg taccggcgaa agtgatcttc tgcgactatg ccggattctc
    29401 aacgcttgat tcggtggtgc gtgccaaaaa ggtgtttggg gaaaaccata tcaccattat
    29461 ttcgcaagaa tttcataacc aacgcgccat ctggctggca aaacagtacg gcatcgacgc
    29521 tatcggtttt aacgcgccag acctcaatat gaagcacggt ttttatactc aactgcggga
    29581 gaaactcgct cgcgtcggcg cggtgattga cgcaaaaatc ctccatcgcc agccgaaata
    29641 tctcgggcca tcggtcatga ttgggccgtt tagtgagcat ggctgtccgg cgcagaaata
    29701 atgcgtatct gcgcacgtcg aagatgaaaa aggcgtgcta cattgacggc agaatccctt
    29761 tatggagtat ccacgcgtta tgatacgttt cgctgtgatt ggtacgaact ggatcactcg
    29821 ccagttcgtc gaggccgccc atgagagcgg taaatacaag ttaaccgccg tatattcccg
    29881 cagccttgaa caggcccagc actttgccaa tgatttttct gtcgagcatc tgtttacgtc
    29941 gctggaagcg atggcggaaa gcgatgccat tgacgcggtg tatattgcca gcccgaatgc
    30001 cctgcacttt tcccagacac aacttttcct tagtcacaaa attcatgtga tttgcgagaa
    30061 gccgctggca tcgaatctgg cggaagtgga tgccgccatt gcctgtgcgc gggaaaatca
    30121 ggtggtgctg tttgaggcat ttaaaacggc ctacctgccg aactttcatc tgttgcgcca
    30181 ggcgctgccg aaagtcggca aactgcgtaa agtctttttc aactattgcc agtattcctc
    30241 gcggtatcaa cgttacctgg atggtgagaa tcccaacacc tttaatccag cattctctaa
    30301 cggttcaatt atggatatcg gcttttactg tctggcgtcg gcggtggcat tattcggtga
    30361 accgaaaaac gtgcaggcaa ccgccagttt gctggcaagc ggtgtggatg cccacggcgt
    30421 ggtggtgatg gattacggtg atttcagcgt tacgttgcag cactcaaaag tgagcgattc
    30481 tgtcctggca agcgagattc agggcgaagc gggatcgctg gtgattgaaa aactgtctga
    30541 atgccagaaa gtgtgttttg tgccgcgtgg cagccagatg caggatctga cccaaccgca
    30601 gcatattaat accatgctct acgaagcaga gctgtttgct gaactggtgg atgagcatct
    30661 ggtggatcat ccagggctgg cggtcagtcg catcaccgct aaactgctga ccgagatccg
    30721 ccgccagact ggggtgattt ttccggcaga tagcgtaaaa ctataattgc caaagtaaaa
    30781 cagtgtaaaa ggtatgtaac agaccattga ctgactgaat ggtctgtcat actttgttac
    30841 ctgcaaaggg gagtaacttc attgccggtc gatcgtcatt acgatgtgtg aaaaaccaca
    30901 tccggtcacc gggcaacccg aaaggaatac gcagacgtat tccttttttg ttgtaagtga
    30961 gaccttgccg gaaggcgagg tctatgcata aaaagcagcg gctgacgtct tccgacgttg
    31021 gccgtttttt tatgtgtaag gaacttctat gaatactgtc ggcacgccgt tgctatgggg
    31081 cggattcgct gttgttgtca ccattatgct ggctatcgac ctgttgttgc aggggcgtcg
    31141 tggggcacat gccatgacca tgaaacaggc tgcggcctgg tcgctggtct gggtgacgct
    31201 gtcgttactg tttaacgccg ctttctggtg gtatctggtg caaaccgaag gtcgcgccgt
    31261 tgccgatcca caggcactgg cctttctcac gggttatctg attgagaaat cgctggcggt
    31321 cgataacgtc tttgtctggc tgatgttgtt cagctatttc tctgttccgg cggcattaca
    31381 acgccgcgtg ctggtgtatg gcgtgctcgg ggcgattgtt ctgcgtacca tcatgatctt
    31441 cactggcagc tggctgattt cacagttcga ctggatcctg tatatcttcg gtgccttcct
    31501 gctgtttacc ggcgtgaaga tggcgctcgc ccatgaagat gaatcaggca ttggcgacaa
    31561 gccgctggtg cgctggctac gcggtcattt gcgcatgacc gacaccatcg acaacgagca
    31621 tttctttgtg cgtaaaaatg gtttgttgta tgccacgccg ctgatgctgg tgctgattct
    31681 ggtggagttg agcgacgtga ttttcgccgt ggatagtatt ccggctatct tcgccgtaac
    31741 caccgatccg tttattgtgc tgacctcaaa cctgtttgcg atcctcggcc tgcgtgcgat
    31801 gtatttcctg ctggcaggtg tggcagagcg tttctcgatg ctcaaatatg gtctggcggt
    31861 gattctggtg tttatcggta tcaagatgct gattgtcgac ttctaccata ttccgatcgc
    31921 cgtctcgctg ggcgtggtgt ttggcattct ggtgatgacg tttattatca acgcctgggt
    31981 gaattatcgg catgataagc agcggggaga atgattcgct gtatattaag ccattactag
    32041 agataactca ttccagacaa aggtgagcta ttaattatgt aatcggctta tagctcttta
    32101 caaaaagacc gcacgggtgt tggtggcgtc aaaatcgccg aagcgttccc tggaggccgg
    32161 ggcgacgcgc atggatgcgc ggcgagggcg catcttacag ggacgttccc ttgcgcccga
    32221 cccggtagcc gcaagggata agctgaggg
//
LOCUS       DAZGQL010000061        31309 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.061, whole genome shotgun sequence.
ACCESSION   DAZGQL010000061 DAZGQL010000000
VERSION     DAZGQL010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31309
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.061"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            146..646
                     /gene="tssB"
                     /locus_tag="ACHKIR_003058"
     CDS             146..646
                     /gene="tssB"
                     /locus_tag="ACHKIR_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HFO9990761.1"
                     /translation="MSKKFEGSVAPRERINISYVPKTDGQTAEVELPLNMLVVGDTGN
                     TQETSPLDERQAVSVNKHNFGAVMAEAAIGLNFTVPATLKGSTTDDELNVALNIKSLD
                     DFSPDSVARQVPEVNKLLELREALTALKGPMGNLPAFRTQLQALLENEESREQLLKEI
                     GQVSGK"
     gene            681..887
                     /locus_tag="ACHKIR_003059"
     CDS             681..887
                     /locus_tag="ACHKIR_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9990762.1"
                     /translation="MAAHKNISGKHPLSDEDILQAVYRVCFSPSGEINRDGVDALITT
                     LDRRISDLLDEIIHLCEFQQSAPY"
     gene            956..2431
                     /gene="tssC"
                     /locus_tag="ACHKIR_003060"
     CDS             956..2431
                     /gene="tssC"
                     /locus_tag="ACHKIR_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HFO9990763.1"
                     /translation="MSLQEEELVSSHAGQTDQASSLLDQIMAQTRIQPGSEGYDVARQ
                     GVTAFIASILQSTASAEPVNKLAVDSMIADIDERISRQMDVIIHAPAFQQVESFWRSL
                     KTMVDRVDFRENIKVNVLHVTKQELLEDFEFAPEIIQSGFYKHVYSSGFGQFGGEPIA
                     AVLGAYEFKNTAPDMKLLQYVSAVGAMAHAPFLSSVSPEFMGLNSWTELPNIKDLYAI
                     FEGPAYTKWRALRDSEDSRYLGLTAPRFLLRQPYSPTDNPVKNFNYYEDVSQNHEDYL
                     WGNTAWMLACNIADSFAKYRWCPNIIGPQSGGAVKDLPVHLFETMGQIQAKIPTEVLV
                     TDRREFELAEEGFITLTMRKDSDNAAFFSANSVQKPKHFPGKDAETNYKLGTQLPYLF
                     IINRLAHYIKVLQREQLGSWKERSDLERELNTWIRQYVADQENPPADVRSRKPLRAAK
                     VEVMDVEGEPGWYQVALSVRPHFKFMGANFELSLVGRLDRE"
     gene            2438..2851
                     /gene="tssE"
                     /locus_tag="ACHKIR_003061"
     CDS             2438..2851
                     /gene="tssE"
                     /locus_tag="ACHKIR_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFO9990764.1"
                     /translation="MIPDRERTTGSLFERMEASSARNRQGGSIHSLRQSIRQNLRNIL
                     NTRSGSCRGAPELGIDEPEGAENFRESMSRAIEQCIERYEPRISHAEVQAVVSSASSP
                     LDMTFHITAWVTFNETHEVLEFDMAPNGSQHYRVD"
     gene            2855..4705
                     /gene="tssF"
                     /locus_tag="ACHKIR_003062"
     CDS             2855..4705
                     /gene="tssF"
                     /locus_tag="ACHKIR_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFO9990765.1"
                     /translation="MEFEERYFREELDYLRQLSKLLATEKPHLARFLAEKDADPDIER
                     LLEGVAFLTGNLRQKIEDEFPELTHGLIKMLWPNYLRPVPAMTLIEYTPDMDKSSVPV
                     LIPRNEQFTTNAGEIRVDEVLPSDAKKEEPPPCTFTLCRDIWLLPVRLEQIENRSTTR
                     NGVINITFSVAPGTDFRTLDLNKLRFWLGNDDNYTRDQLYLWFCEYLQGADLTVGEQH
                     LRLPEFMLKAVGFEPQDAMLPWPKNVHSGYRILQEYFCYPDAFLFFDLCGCPALPDGL
                     QAEFFTLQLRFSHPLPVDIRLRRDSLRLYCAPAINLFIHHAEAITLDNRRADYPLVPS
                     RHYPEHYDVFSVNGVISQVQDMFRKKDLGRPVSTQAARQWPAFESFSHQMEYSRKREV
                     VYWHHRTKTSLFHRCFDHTLAFIHADGSYPSDESLLSNEVVSVSLTCTNRELPSQIRS
                     GDITGTTGKNAAVASFRNITRPTQPLWPVIDGSLHWSLLSAMNLNYLSLLDTDALKQV
                     IANFDRHAIHHPQTARLSQQKLDAIERLETRPVDRLFTGIPVRGLASTLYLHPEPFVC
                     EGEMYLLGTVLSHFLSLYASVNSFHMLTVVNTESQETWKWTERTGQHPLI"
     gene            4669..5751
                     /gene="tssG"
                     /locus_tag="ACHKIR_003063"
     CDS             4669..5751
                     /gene="tssG"
                     /locus_tag="ACHKIR_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFO9990766.1"
                     /translation="MDGKNRAASSYLSPGNPPADKEQNDPLAQVFHNACSYNFFAMAE
                     LLHRLAKGEKGTPELSLRDDPAQETLRFSADASLAFPCSDISALKRDTSGAFRMTTTF
                     MGLQGSQSPLPGYYLDHLAWKAVHEQSPVGDFLDMFSHRLTQFVWHIWRKYRYHISFR
                     NGGVDAFSQRMYSLVGLGHRQLRDKLAINHSKMLAYSGILANPGRSPEIICGLVSHCF
                     DLSDVTLQNWQRRKVDIEPDQQNSLGSYSLKNGEKLAGRSVLGNFVLGTRVPDLSGKF
                     QLSITSLTRKQFLSFLPSGENFLPLTMFVSFILRDQLAWDLHLGLAPEQVGAMRLGDN
                     KSALLGWTSFLGTPEERPSVTIRVRS"
     gene            5776..7056
                     /gene="tagH"
                     /locus_tag="ACHKIR_003064"
     CDS             5776..7056
                     /gene="tagH"
                     /locus_tag="ACHKIR_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HFO9990767.1"
                     /translation="MPEEKLQTLSLQVINGSELESGRAARCVFTQQGNVGHGPECQWS
                     VQDRQQSIPAQAFTVILHDGTFCLRPQTAQLWLNQAKVTATSDLIQLRQGDEIQIGRL
                     MVRVHLNRGDIPHYDEEMATPETIVTNRDMLTDTLLSTEGAPHYPGMTHRHQLADTVV
                     NGFSTDPLQALQSESLITAGDPLSGIAAVRPSAPLSDPASNGGINTPFMDLPPIYASP
                     GDRNDDVSVAEMAQRHLAVTPLLRGLGGSLTVSNSDDADDFLEEAGRTLQAAIKGLLD
                     LQQQRNSLSDKHLRPLEDNPLRLNMDYATALDVMFAEGKSPVHLAAPAAVSESLRNVR
                     HHEEANRAAIVESLRVLLDAFSPQNLLRRFVQYRRSHELRQPLDDAGAWQMYSHYYEE
                     LASDRQQGFEMLFNEVYAQVYDRVLREKQREPEA"
     gene            7053..7577
                     /gene="tssJ"
                     /locus_tag="ACHKIR_003065"
     CDS             7053..7577
                     /gene="tssJ"
                     /locus_tag="ACHKIR_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFO9990768.1"
                     /translation="MNRKAFLACVLMCVILTGCETAKKISEVIKNPDIQVGSLKEQPS
                     EITVTLLTEPDTNTNAEGESAAVDVQLVYLTDDSKLLAADYDQIASTPLPDVLGKNYI
                     DHQDFNLLPDTIKTLPPVKLDEKTQFIGVVAYFSDDQATEWKQIETVEGTGHHYRLLV
                     HVRQSSIEMKKEDE"
     gene            7580..8911
                     /gene="tssK"
                     /locus_tag="ACHKIR_003066"
     CDS             7580..8911
                     /gene="tssK"
                     /locus_tag="ACHKIR_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HFO9990769.1"
                     /translation="MATTRNKVMWQEGMLMRPHHFQQQQRYNDYLDNQRFRAMNDLSW
                     GFTELTLNNELLAQGKIMIDSASGILPDGTVFSIPDQDALPDPLHPQNFPDERSRNIY
                     LALPVASDVRNEISDGRRIGRYRLNYADVRDLHSEEGDARTLTLGQLTPRIMSGAEDM
                     SAYITLPLCRISDRHADGSLTLDDDFIPSCQNIQVSKKLRVYLKEVQGAIGGRASDLA
                     NRIGSPAQSGIADVAEFMMLQLLNRNQTRFTHRARRSQLHPEDFYLDLAGLLGELMTF
                     TEPSRLPCPLDVYDHHDLTKTFKTLLPEVKRALHTVLSPRAVNLPLHLRDGIWQADIH
                     DTELLQSATFVLAVAANMPVDQIQRQFIQQSKISSPEKIRNMVSVQIPGIPLRALMVA
                     PRQLPYHSGFSYFELDKSGQAWAEIAAAGAVALHVSGSFPDLNMQLWAIRG"
     gene            8916..9677
                     /gene="icmH"
                     /locus_tag="ACHKIR_003067"
     CDS             8916..9677
                     /gene="icmH"
                     /locus_tag="ACHKIR_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HFO9990770.1"
                     /translation="MDEGSLSLPPFTGHDEKSQRDYHLALRGNSLNPMIDAATPLLGM
                     VMRLSTMNSQTMPEHLFAQVVTDVQAVEQLLQEQGYEPGVIISFRYILCTFIDEAALG
                     NGWSNKNEWIKQSLLVHFHNEAWGGEKVFILLERLIREPKRYQDLLEFLWLCFSLGFR
                     GRYKVAAQDQGEFEQIYRRLYHVLHKLRGDAPFPLLHQDKKTQGGRYQLISRLTIKHI
                     FYGGVVVLALFYLFYLLRLDSQTQDILHQLNKLLR"
     gene            9686..12469
                     /gene="tssH"
                     /locus_tag="ACHKIR_003068"
                     /gene_synonym="clpV"
     CDS             9686..12469
                     /gene="tssH"
                     /locus_tag="ACHKIR_003068"
                     /gene_synonym="clpV"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HFO9990771.1"
                     /translation="MIQIDLPTLVKRLNLFSRQALEMAASECMSQQAAEITVSHVLIQ
                     MLAMPRSDLRVITRQGDIGMEELRQALTVENYTTARSADSYPAFSPMLVEWLKEGWLL
                     ASAEMQHSELRGGVLLLALLHSPLRYIPPAAARLLTGINRDRLQQDFVQWTQESAESV
                     VPDADGKGAGTLTDAADTLLARYAKNMTADARNGRLDPVLCRDHEINLMIDILCRRRK
                     NNPVVVGEAGVGKSALIEGLALRIVAGQVPDKLKNTDIMTLDLGALQAGASVKGEFEK
                     RFKGLMAEVISSPVPVILFIDEAHTLIGAGNQQGGLDISNLLKPALARGELKTIAATT
                     WSEYKKYFEKDAALSRRFQLVKVSEPNAAEATIILRGLSAVYEQSHGVLIDDDALQAA
                     ATLSERYLSGRQLPDKAIDVLDTACARVAINLSSPPKQISALTTLSHQQEAEIRQLER
                     ELRIGLRTDTSRMTEVLVQYDETLTALDELEAAWHQQQALVQEIIALRQQLLGVAEDD
                     AAPLPDADTVEDTPPESESESESESEQDNTGAVPADEADREQPEETAETVSPVQRLAQ
                     LTAELDALHNDRLLVSPHVDKKQIAAVIAEWTGVPLNRLSQNEMSVITDLPKWLGDTI
                     KGQDLAIASLHKHLLTARADLRRPGRPLGAFLLAGPSGVGKTETVLQLAELLYGGRQY
                     LTTINMSEFQEKHTVSRLIGSPPGYVGYGEGGVLTEAIRQKPYSVVLLDEVEKAHPDV
                     LNLFYQSFDKGEMADGEGRLIDCKNIVFFLTSNLGYQVIVEHADDPETMQEELYPVLA
                     DFFKPALLARMEVVPYLPLSKETLATIIAGKLARLDNVLRSRFGAEVVIEPEVTDEIM
                     SRVTRAENGARMLESVIDGDMLPPLSLLLLQKMAANTAIARIRLSAVDDAFTADVEDA
                     QNDESVTKDETVL"
     gene            12466..13209
                     /gene="vasI"
                     /locus_tag="ACHKIR_003069"
     CDS             12466..13209
                     /gene="vasI"
                     /locus_tag="ACHKIR_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     VasI"
                     /protein_id="HFO9990772.1"
                     /translation="MSRLRVFRQTGWFIAGLMTGLPATAAPAETSSMAGVAVAVATTP
                     PPDATATLQAMQSCRRESAALERLDCYDRLLAPLSPSGFDGALVKAGFVGEAWTRATE
                     QEKRREGNTTELLVTQVPGERPTVVITTPAIGHVPPRPVLMFSCVDNITRMQVALMHP
                     LDVHDIAVTLNADSRALRSHWFVRENGTLLESSRGLSGIDEIKQLFGAKTLTVDTGAD
                     NAAGKLTFNIDGLARAIAPLRVACHWAGE"
     gene            13214..14626
                     /gene="tssA"
                     /locus_tag="ACHKIR_003070"
     CDS             13214..14626
                     /gene="tssA"
                     /locus_tag="ACHKIR_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HFO9990773.1"
                     /translation="MAMNLRDPNVWISHLLENLPEEKLASALKDDNPDWEYIDGEIVK
                     LGSLAHSQLDIPELQRRGLVILASESKDFRLLAHLLRTLQHAGDPHLALRLLALYVEH
                     YWSVAAPQNMAHKKRFASQVIKRFETGIEGFSQNAATAQHDALSGELAKLAQCWQSYN
                     APELALATDDLFALYQRAFNRAAPAPAPTPAASDSSRQTTATSESGVTQPSAPAPQIA
                     IDSHDDKAWRDTLLKVAAILCERQPDSPQGYRLRRHALWQSITSTPQAESDGRTPLAT
                     VSADMVADYQSRLASADMALWQQVEKSVLLAPYWLDGHCLSAQTALRLGYKQVADTIR
                     DEVIRFLERLPQLTGLLFNDRTPFLSEQTKQWLAASPDGKVAPVAQIGEESQAARACF
                     AGQGLEAALRYLDMLPEGDPRDQFHRQYLAAQLTEEAGLIQLAQQQYRMLLMIGSQMM
                     VSDWEPSLLTQLEQKFTAEQ"
     gene            14735..18169
                     /gene="tssM"
                     /locus_tag="ACHKIR_003071"
     CDS             14735..18169
                     /gene="tssM"
                     /locus_tag="ACHKIR_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HFO9990774.1"
                     /translation="MLILAWIFLLVWIWWKGPTWTLYEEQWLKPLANRWLATAAWGII
                     ALVWLTVRVMKRLQQLEKMQKQQREEAIDPLSVELNAQQRYLDRWLLRLQRHLDNRRF
                     LWQLPWYMVIGPAGSGKTTLLREGFPSDIIYAPEGARGAEQRLYLTPHVGKQAVIFDI
                     DGTLCAPADADILHRRLWEHALGWLKEKRARQPLNGIILTLDLPDLLTADKRRREHLL
                     QTLRSRLQDIRQHLHCQLPVYVVLTRLDLLQGFAALFQSLNRQDRDAILGVTFTRRAH
                     ENDDWRTELNAFWQTWVDRMNLALPDLMVAQTHTRASLFSFSRQMQGSREPLVSLLEG
                     LLDGENMNVMLRGIYLTSSLQRGQMDDIFTQSAARQYRLGNNPLASWPLVDTAPYFTR
                     GLFPQALLAEPNLATESRTWLMRSRRRLTVFSTTGGVAALLLITGWHHYYNGNYQSGI
                     TVLKQAKAFMDVPPPLGEDDYGNLQLPLLNPVRDATLAYGDWGDRSRLADMGLYQGRR
                     IGPYVEQTYLQLLEQRYLPSLFNGLVKAMNAAPPESEEKLAVLRVMRMLEDKSGRNNE
                     VVKQYMAKRWSEKFHGQRDIQAQLMSHLDYALAHTDWHAERQAGDGDAISRWTPYDKL
                     VVSAQKELSKLPVYQRVYQSLKTRALGVLPADLNLRDQVGPTFDQVFTSADDNKLVVP
                     QFITRYGLQSYFVKQRDELVELTAMDSWVLNLTRSVKYSDADRAEIQHQLTEQYISDY
                     TATWRAGMDNLNIRNFESIGQLTGALEQVISGDQPLQRALTVLRDNTQPGVFSEKLSA
                     KEREEALAEPDYQLLTRLGHEFAPENSTLAVQKDKESTMQAVYQQLTELHRYLLAIQN
                     APVPGKSALKAVQLRLDQNSSDPIFATRQMAKTLPAPLNRWVGRLTDQAWHVVMVEAV
                     HYMEVDWRDSVVKPFNEQLANNYPFNPRSAQDASLDAFERFFKPDGILDTFYQQNLKL
                     FIDNDLSLEDGDNNVIIREDIIAQLETAQKIRDIFFSKQNGLGTSFAVETVSLSGNKR
                     RSVLNLDGQLVDYSQGRNYTAHLVWPNNMREGNESKLTLIGTSGNAPRSISFSGPWAQ
                     FRLFGAGQLTGVQDGNFTVRFSVDGGAMTYRVHTDTEDNPFSGGLFSQFGLSDTLY"
     gene            18180..19590
                     /locus_tag="ACHKIR_003072"
                     /pseudo
     CDS             18180..19590
                     /locus_tag="ACHKIR_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308244.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
     gene            19556..20035
                     /locus_tag="ACHKIR_003073"
     CDS             19556..20035
                     /locus_tag="ACHKIR_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFO9990775.1"
                     /translation="MANLIYLTLNGDNQGLISSGCSSQPSIGNKAQTAHIDQIMIYEF
                     MHGLNRDQNVNHHHLTIKKPIDKASPLLGKAICDNELLTCDFSFYRTNKFGINELYYK
                     IKLTGAKISDIHVSISHIVVDNSIQPEESVSFSYESIIWEHCSAGTSAYSLWEDRLF"
     gene            20056..20274
                     /locus_tag="ACHKIR_003074"
                     /pseudo
     CDS             20056..20274
                     /locus_tag="ACHKIR_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_302948.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            20356..20952
                     /locus_tag="ACHKIR_003075"
     CDS             20356..20952
                     /locus_tag="ACHKIR_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HFO9990776.1"
                     /translation="MLWTENDAANTSQWNGYPLQIGRFRKDKAMPALISGEKSAALVT
                     PPQWRNKAFNGLKDPERNYWAKEQITGSPEENIKAAITYLMMKLSNTKEESTIDQYDS
                     TLYSVIVQKGDLADNIRKERKTTIPNLTKNNPGKNLDKIHPGDILYYQKASMKVIITG
                     WKPITIKNVAMNYNGGGDPKYAIKLQFVYTLLTKNRVL"
     gene            20955..21404
                     /locus_tag="ACHKIR_003076"
     CDS             20955..21404
                     /locus_tag="ACHKIR_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1311 domain-containing protein"
                     /protein_id="HFO9990777.1"
                     /translation="MGKITIILLLTFSCTKCLAFDISMSKECSGVDELEFVDCVKNSF
                     IMSTITLKKAEVNIKEKIKKWNNDEDEAIRKEAVISLKRLESSNREFIRYRFAQCSFA
                     LTWGARVGRLAFSRLYPCYTELNKIRAMQLNNAYISDFQNPDEISSK"
     gene            21401..21751
                     /locus_tag="ACHKIR_003077"
     CDS             21401..21751
                     /locus_tag="ACHKIR_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000583955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9990778.1"
                     /translation="MIIIKLTLLFILATITYSVQSDEFDDQTTYTQYRYFSTAIPEDI
                     RRFMFYVDYCFVDLAFDIETDSMTAKNDIINECTPLKERNKTLKEKYKKDEEIIAILS
                     IADDTILIYEELKK"
     gene            complement(21876..22676)
                     /locus_tag="ACHKIR_003078"
     CDS             complement(21876..22676)
                     /locus_tag="ACHKIR_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9990779.1"
                     /translation="MNSKKVCCICVLFSLLAGCASQSSIDEKKKKAQVTQSNINKNIP
                     PQLTDKDLFGNETTLAVSEEDIQAALEGDEFRVPLNSPVILVQSGSRAPETIMQEEMR
                     KYYTVSTFSGIPDRQKPLTCNKNKDKNENEDVASAENMNWMQALRFVAAKGHQKAIIV
                     YHDTLQTGKYDSALKSTVWSDYKNEKLTDAISLRYLVRFTLVDVATGEWATWSPVNYE
                     SRVIFPPAGITKMSNKELSNDHVTEVQLFNLKQKTYVSMVKDLVSRFQ"
     gene            complement(23004..23080)
                     /locus_tag="ACHKIR_003079"
     tRNA            complement(23004..23080)
                     /locus_tag="ACHKIR_003079"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(23044..23046),aa:Asp,seq:gtc)
     gene            complement(23213..23944)
                     /gene="dnaQ"
                     /locus_tag="ACHKIR_003080"
     CDS             complement(23213..23944)
                     /gene="dnaQ"
                     /locus_tag="ACHKIR_003080"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HFO9990780.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQDETTIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            24009..24476
                     /gene="rnhA"
                     /locus_tag="ACHKIR_003081"
     CDS             24009..24476
                     /gene="rnhA"
                     /locus_tag="ACHKIR_003081"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFO9990781.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(24473..25195)
                     /gene="yafS"
                     /locus_tag="ACHKIR_003082"
     CDS             complement(24473..25195)
                     /gene="yafS"
                     /locus_tag="ACHKIR_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO9990782.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGLPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            25229..25984
                     /gene="gloB"
                     /locus_tag="ACHKIR_003083"
     CDS             25229..25984
                     /gene="gloB"
                     /locus_tag="ACHKIR_003083"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HFO9990783.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVKKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            26056..27414
                     /gene="mltD"
                     /locus_tag="ACHKIR_003084"
     CDS             26056..27414
                     /gene="mltD"
                     /locus_tag="ACHKIR_003084"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HFO9990784.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(27461..28231)
                     /locus_tag="ACHKIR_003085"
     CDS             complement(27461..28231)
                     /locus_tag="ACHKIR_003085"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO9990785.1"
                     /translation="MTTQSHHDHVEKQFSSQASEYLTSTVHASGRDLQRLAVRLADYP
                     DASVLDMGCGAGHASFVAAQNVSAVVAYDLSAQMLDVVAQAAEARHLKNIATRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRLIVMDVMSPGHPVRDVWL
                     QTVEALRDTSHVRNYASGEWLTLINEANLIVDNLITDKLPLEFSSWVARMRTPEALVD
                     AIRIYQQSASTEVKTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(28308..29108)
                     /gene="yafD"
                     /locus_tag="ACHKIR_003086"
     CDS             complement(28308..29108)
                     /gene="yafD"
                     /locus_tag="ACHKIR_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFO9990786.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            29349..30263
                     /gene="yafC"
                     /locus_tag="ACHKIR_003087"
     CDS             29349..30263
                     /gene="yafC"
                     /locus_tag="ACHKIR_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YafC"
                     /protein_id="HFO9990787.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDREIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(30260..31063)
                     /gene="dkgB"
                     /locus_tag="ACHKIR_003088"
     CDS             complement(30260..31063)
                     /gene="dkgB"
                     /locus_tag="ACHKIR_003088"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HFO9990788.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
     gene            complement(31225..31301)
                     /locus_tag="ACHKIR_003089"
     tRNA            complement(31225..31301)
                     /locus_tag="ACHKIR_003089"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31265..31267),aa:Asp,seq:gtc)
BASE COUNT         7973 a         8053 c         8064 g         7219 t
ORIGIN      
        1 atttattttg aagtgtagaa tgcggggtga tgaaggagtg gtaattttat tttcaccgcc
       61 attagggcgg cattgccgat atttttttgc ggtacaaata tgccgtgttc atggcctgta
      121 cgagataaca tgacggatca cttttatgag caaaaaattt gaaggcagcg tcgcgcctcg
      181 cgagcgtata aatatatcct atgtcccgaa gacggacggc cagacggctg aagtagagct
      241 tccgcttaac atgctcgttg tcggcgatac cggaaatacg caggagacat ccccactgga
      301 tgaacgtcag gcagtttccg ttaacaagca caatttcggt gcagtaatgg ctgaagcagc
      361 cattggcctc aattttaccg tccccgccac gctgaaggga agcacaacgg acgatgaact
      421 gaatgtggcg ctgaatatca agtcactgga tgatttttca ccggacagcg tggcccggca
      481 ggttccggaa gtgaataaat tgctggaact gcgtgaagca ctgactgcgc tgaaggggcc
      541 gatgggcaac cttccggcat tccgtaccca gctccaggca ttgctggaaa atgaggagtc
      601 ccgcgagcag ttgcttaaag aaatcggcca ggtgagtggg aaataaccac cactatttca
      661 ttaaacaggg ttatccagac atggcggcac acaaaaatat ctccgggaaa caccccctct
      721 ctgacgaaga cattttgcag gcggtttacc gggtatgttt cagcccgtcc ggtgaaatca
      781 accgggatgg cgtggatgcg ctgattacaa cactggatcg ccgtatcagt gatctattgg
      841 atgagattat ccatctttgt gagtttcagc agagcgcgcc gtactgacgg cgcgtacttc
      901 actgacggcg aaaacccggt ttcagaaaca cagctaaaac agaaggattt tcagtatgtc
      961 tttacaggaa gaggaactcg tttccagtca tgccgggcag actgatcagg cgtcgtcgct
     1021 gcttgatcaa atcatggcac agacccgtat tcagcctggt tcggaaggtt atgacgttgc
     1081 ccgccagggt gtgacagcct ttattgccag catcctgcaa tccactgcat ctgccgaacc
     1141 ggtcaataaa ctggccgtgg acagcatgat tgcagatata gatgaacgca tcagccgcca
     1201 gatggatgtc atcatccatg cgcctgcctt tcagcaggtc gagtcgttct ggcgctcact
     1261 gaaaaccatg gtggatcgcg ttgatttccg tgaaaacatc aaggtcaacg tgcttcatgt
     1321 cacgaagcag gaacttctgg aagactttga atttgcccca gaaattattc agtccggatt
     1381 ttataaacac gtttattcgt ccggattcgg acagtttggt ggtgaaccca tcgctgccgt
     1441 gctgggcgct tatgagttca aaaataccgc tcctgacatg aaactgctgc aatacgtcag
     1501 tgccgtgggc gcaatggcgc acgcaccgtt cctgtcctcc gtttccccgg agtttatggg
     1561 gctgaactcg tggaccgaac tgcctaatat caaagatctt tatgccatct ttgaaggtcc
     1621 ggcctacacc aaatggcgcg ccctgcgtga ctcggaagat tcgcgctatt tagggcttac
     1681 ggcaccccgc ttcctgcttc gccagcccta ttcaccaaca gataaccctg ttaagaactt
     1741 taactattac gaagatgtca gccagaacca cgaagattat ctgtggggta atacggcctg
     1801 gatgctggca tgcaatatcg cagacagttt tgccaaatac cgctggtgtc caaacattat
     1861 cggtccgcaa agcggcggcg cagtgaaaga tctgccggtg catctgttcg aaacgatggg
     1921 gcagattcag gccaaaatcc caaccgaggt gctggtcacc gatcgccgcg aatttgaact
     1981 ggctgaagag ggttttatca cccttaccat gcgtaaagac tctgataatg cagccttttt
     2041 ctctgcaaac tcggtacaaa aaccgaagca ctttccggga aaagacgcag aaaccaacta
     2101 taagctgggt acgcagcttc cttatctctt catcatcaat cggttagcgc actacatcaa
     2161 ggtgttgcag cgtgaacaac tggggtcatg gaaagaaagg agcgacttag aaagagagct
     2221 taatacctgg atccgtcagt atgttgccga ccaggaaaac ccgcctgcag acgtgcgcag
     2281 ccgcaaacct ctgcgcgctg ccaaagttga ggttatggat gtagaaggcg aacccggctg
     2341 gtaccaggtt gcgctaagcg tgaggcctca ttttaaattt atgggggcaa attttgagct
     2401 ttccctggtt ggccggttag acagggagta acacaccatg ataccggatc gggaaagaac
     2461 cacaggttct ctttttgagc gtatggaagc ctcttcagcc agaaaccggc agggaggcag
     2521 tattcattcg ctcaggcaat ccattcgcca gaacctacga aacattctta atacccggtc
     2581 cggtagttgc cggggtgcgc ctgagctggg cattgatgaa ccggaagggg ctgaaaactt
     2641 ccgcgaatca atgagtcggg ccatcgagca atgtatagaa cgctatgaac cccggatctc
     2701 acacgctgaa gtgcaggcag tggtttcatc cgcctccagc ccgctggata tgacgtttca
     2761 tattacagcc tgggtgacat ttaacgaaac gcatgaggtg cttgaatttg atatggcacc
     2821 gaatggcagc cagcattacc gggtggactg acaaatggaa tttgaagaac gctatttccg
     2881 ggaagaactc gattacctgc gccagcttag caagctgctg gcaacggaaa aaccccatct
     2941 ggcccgcttc ctggccgaaa aagatgcaga tccggatatt gaacgcctgc tggaaggggt
     3001 ggcttttctt accggcaatc tccgccagaa aattgaggat gaattcccag aactgacgca
     3061 cgggcttatt aagatgctct ggcctaatta cctgcgtccg gttccggcaa tgacccttat
     3121 tgaatatacg ccggatatgg ataagtcttc tgtaccggtg ttaatccccc gtaatgagca
     3181 gtttacaacc aacgccgggg aaatcagagt tgatgaagtg ctgccctctg atgctaaaaa
     3241 ggaggagcct cctccctgta ccttcaccct ctgccgggat atctggctgc tgcccgttcg
     3301 cctggagcag attgaaaacc gcagtacgac ccgtaatggt gttatcaaca tcaccttttc
     3361 ggtcgcaccg ggaacggact tccgcacgct ggatctgaac aaacttcgct tctggctcgg
     3421 caatgacgac aactataccc gtgaccagct ttatttatgg ttctgcgaat acttgcaggg
     3481 tgctgacctg actgtgggtg aacagcatct tcgcctgcct gagtttatgc taaaagctgt
     3541 cggttttgag ccgcaggatg ccatgctgcc ctggccgaaa aacgtccaca gcggctaccg
     3601 gatccttcag gagtatttct gttaccccga tgcgtttctc ttttttgatc tttgtggttg
     3661 tccggctttg cctgacggat tgcaggcgga attctttacc ctgcaactgc gtttttcgca
     3721 cccattgccc gtggacatcc ggttgcgccg cgactccctg cgcctgtatt gcgcgcctgc
     3781 cattaattta tttatccacc atgcagaagc catcacgctg gacaaccggc gggcagacta
     3841 tccgctggtt cccagccgcc attatccgga acattacgat gtcttttccg ttaacggcgt
     3901 gataagccag gtgcaggata tgttcaggaa aaaagatctg ggtcgtcctg tttcgacgca
     3961 ggccgcgcgc cagtggcctg cctttgaaag tttcagccat cagatggaat acagccggaa
     4021 gcgggaagtg gtgtactggc atcaccggac caaaacatcc ctgttccatc gctgctttga
     4081 tcataccctt gcttttatac atgctgatgg cagttatccg tcagacgaat cgctgctcag
     4141 taatgaagtg gtttcggtat cgctgacctg taccaaccgt gaacttccgt cacaaattcg
     4201 ttccggcgat atcaccggca caaccggtaa aaatgcggct gttgcttcat ttcgcaacat
     4261 tacccgcccg acgcaaccgc tctggccggt cattgatggc agcctgcact ggtccctgct
     4321 ctccgccatg aacctgaatt atctgtcatt actggatacg gacgcgctga agcaggtcat
     4381 cgccaacttt gatcgccacg caatccatca tccgcagacg gcgcggctgt cacaacaaaa
     4441 gctggatgcc attgagcgtc tggagacccg ccctgtcgat cgcctgttta cgggcattcc
     4501 cgtccggggg ctggcctcca cgctgtatct gcacccggag ccgtttgtct gtgaagggga
     4561 aatgtatctg ctcggtacgg tgctttcgca ttttctgtcg ctgtacgcca gcgttaactc
     4621 attccacatg ctgaccgttg tgaacacaga aagccaggag acatggaaat ggacggaaag
     4681 aaccgggcag catcctctta tctgagtccg ggaaatcccc cggcagataa agagcagaat
     4741 gatccgctgg cacaggtttt tcataacgcc tgctcttaca atttttttgc gatggcggag
     4801 ctgttgcacc gcctggcaaa gggtgaaaag gggacgccag aattatccct gcgtgacgat
     4861 ccggcacagg aaaccctgcg tttcagcgcc gatgccagcc ttgccttccc gtgcagtgat
     4921 atcagtgcgc tgaaacggga tacgtcaggg gcattcagga tgaccaccac ctttatgggg
     4981 ttacagggga gccagtcgcc tctccccggt tattacctcg atcacctggc ctggaaagcg
     5041 gtacatgaac aaagtccggt aggtgatttt ctggatatgt tcagccaccg cctgacccag
     5101 tttgtctggc atatctggcg taagtaccgc tatcacatca gttttcgtaa cggcggtgtg
     5161 gatgcgttct cgcagcgcat gtactccctt gtcgggctgg gccaccgcca gcttcgcgat
     5221 aagctggcga ttaatcacag caaaatgctg gcgtattccg ggatcctggc aaatccggga
     5281 cgatccccgg aaataatctg cggcctggtg tcccactgct ttgatttgtc agacgtgacg
     5341 ctgcaaaact ggcaacgtcg caaggtggat attgaaccgg atcagcaaaa cagtctgggg
     5401 agctattcac ttaaaaatgg cgaaaaactg gcgggaagat cggtactggg caactttgtt
     5461 ctgggtacac gggtgcccga tctcagcgga aaattccagc tcagtattac cagtctgacc
     5521 cgaaaacagt tcctctcttt tctgccgtcc ggcgaaaact tcctgccact gacgatgttt
     5581 gtgtccttca tcctgcgcga tcagcttgcc tgggacttac atctggggct ggcgccggag
     5641 caggtgggcg caatgcgcct gggcgataac aaaagtgcgc tgctgggctg gaccagcttc
     5701 ctcggcacac cggaagaacg accctcagtc accatcaggg tacggtcgta aattatgata
     5761 atcacggagc agaatatgcc ggaagagaaa ctacagacgc tttcattgca ggtcattaac
     5821 ggcagtgagc tggaaagcgg gcgggcggcg cgctgtgtgt tcacacagca gggaaatgtc
     5881 ggccatggcc ccgaatgcca atggtcggta caggatcgtc agcagagcat tccggctcag
     5941 gcttttaccg ttatcctgca cgatggcaca ttttgtctgc gcccgcagac ggcacaactg
     6001 tggctgaacc aggcaaaagt cacagcaaca tcagacctga tacagctgcg ccagggcgat
     6061 gagatccaga tagggcgtct gatggtgagg gttcatctga accggggaga tatcccccat
     6121 tacgatgagg aaatggccac tcccgaaacc atcgtcacca atcgcgatat gctcacggat
     6181 accctgctgt caacggaggg tgcgccacac tatccgggaa tgactcaccg gcatcagctt
     6241 gcagacaccg tggtaaacgg tttttctacc gatccactcc aggcacttca gtccgaaagc
     6301 ctgattaccg cgggcgatcc gctttcaggc attgcggctg tccggccatc ggcaccgctg
     6361 tccgatccgg caagtaatgg ggggatcaat actccgttta tggatctgcc gcccatttat
     6421 gccagccccg gcgatcgtaa tgatgacgtc tctgtggcag aaatggcgca acgccacctt
     6481 gcggtcaccc ccttactgcg cggtctcggc ggttcgctta ccgtgagcaa ttccgacgat
     6541 gcggatgatt ttctggaaga ggccggacgc acgttacagg ccgcaataaa aggtctgctc
     6601 gatttgcagc aacagcgtaa cagcctctca gacaaacatt tgcgcccgct ggaagataac
     6661 ccgctgcgcc tgaacatgga ttacgccacc gcgctcgacg tgatgtttgc cgaaggtaaa
     6721 agtccggtac atctggcggc tcccgccgct gtcagtgaaa gcctgcgcaa tgtccgccac
     6781 cacgaagaag ctaaccgggc agcgattgtg gagtcacttc gtgtcctgct tgacgctttc
     6841 tcaccacaaa atctgctgcg ccgctttgtg cagtaccgcc gcagccatga actgcgccag
     6901 ccgctggatg atgccggagc atggcaaatg tacagccatt attacgaaga actggcctcc
     6961 gatcgccagc aggggtttga gatgctgttt aacgaggtct acgcccaggt ctatgaccgg
     7021 gtgcttcgtg aaaaacagcg ggagccggaa gcatgaacag gaaggcgttt ctggcctgcg
     7081 tgctgatgtg cgtcatatta accggctgcg aaacggcgaa aaaaatcagc gaggtgatca
     7141 aaaacccgga tatccaggtc gggagcctga aggagcaacc ttcggaaata accgtcaccc
     7201 tgctgaccga accggacacc aataccaatg cggagggtga atcagccgcc gtggatgtgc
     7261 aactggtgta cctgactgac gactcaaaat tgctggcagc cgactatgac cagatagcca
     7321 gtacgccgct accggatgtg ttgggtaaaa actatatcga ccaccaggat ttcaacctgt
     7381 tgccggacac catcaaaacc ctgccgccgg taaagctgga tgagaaaacg cagtttattg
     7441 gtgtcgtggc ctatttctcc gatgaccagg ccactgaatg gaaacaaatc gagacggtgg
     7501 aaggaaccgg ccaccattac cgcctgctgg tgcatgtacg tcaaagcagc atcgaaatga
     7561 aaaaagagga tgaataatta tggcaaccac ccgcaacaag gtgatgtggc aggaagggat
     7621 gctgatgcgc ccacaccatt tccagcaaca gcagcgttac aacgactacc tggataacca
     7681 gcgtttccgg gccatgaatg atttatcctg gggatttacc gaactcaccc tcaacaatga
     7741 actgctggcg cagggtaaga tcatgattga cagcgcgtca ggcatactgc ccgacggcac
     7801 cgtcttttct atccccgacc aggacgcact gcccgatccg ctgcacccgc aaaattttcc
     7861 ggacgaaaga agccgcaata tttaccttgc tctgccggtc gccagtgatg tgagaaatga
     7921 aatcagcgac gggcggcgaa tcgggcgtta ccggctgaat tatgccgatg tccgggatct
     7981 gcattcagaa gaaggtgacg cgcgaacact gacgctgggt caactgacgc cgcgcattat
     8041 gagcggtgca gaagatatga gcgcctatat tacgctcccg ctttgccgta tcagcgatcg
     8101 ccatgccgac ggctccctga cgctggatga cgattttatc ccctcctgcc agaatattca
     8161 ggtcagtaag aaactgcgtg tttatctcaa agaggtacaa ggggccattg gcggacgggc
     8221 aagcgatctg gcaaaccgca ttggctctcc ggcgcagagc ggcatcgcgg atgtggcgga
     8281 atttatgatg ttgcagttac ttaaccgtaa ccagacccgg tttacccatc gcgctcgtcg
     8341 atcccagctc cacccggaag atttctacct tgatcttgcc gggttgctgg gtgaactgat
     8401 gacctttacc gagccgtcgc gcctgccctg cccgcttgat gtgtatgatc atcatgacct
     8461 gaccaaaaca tttaaaacgc tgttaccgga agtcaaacgg gcgctgcata ccgtactgtc
     8521 gccaagagcc gtcaatctgc cgctgcatct gcgtgacggt atctggcagg ccgatattca
     8581 tgacacagaa ctgctgcaat ctgccacctt tgtgctggcc gtggcggcaa acatgccggt
     8641 cgatcagatc cagcgccagt ttatccagca gtcgaaaatt tcctcgccgg aaaaaatccg
     8701 caatatggtc agtgtgcaga tacccggcat tccattgcgt gccctgatgg tggctccccg
     8761 ccagcttcct taccattccg ggttcagcta tttcgaactc gacaaaagcg gccaggcctg
     8821 ggctgaaata gctgccgccg gggccgttgc actgcatgtt tccggcagtt tcccggatct
     8881 gaacatgcaa ctgtgggcga taagagggta agacgatgga tgaggggtca ctgtctctgc
     8941 caccatttac cggacacgac gaaaaaagcc agcgcgacta ccatctcgcg cttcgtggca
     9001 acagcctgaa tccgatgatc gacgcggcca cgccgctact cggtatggta atgcgccttt
     9061 caaccatgaa cagccagacg atgccggagc atctttttgc tcaggtggtg acggatgttc
     9121 aggccgtgga gcaactgctt caggagcagg gctacgaacc gggcgtgatt atctcgtttc
     9181 gctacatcct ctgcacgttt attgatgaag cggcgctcgg taacggctgg tcgaacaaga
     9241 acgagtggat caaacagtca ttgctggtcc atttccacaa cgaagcctgg ggtggagaga
     9301 aagttttcat cctgctggag cgcctgatcc gtgaacccaa acgttaccag gatctgctgg
     9361 aatttctctg gctctgtttt tctctgggat ttcgcgggcg ctacaaagtg gcagcacagg
     9421 atcaggggga atttgagcag atctaccgcc gcctgtacca cgtcctgcat aaactacgcg
     9481 gcgatgcccc tttcccgctg ttacaccagg acaaaaaaac ccagggcggg cgttatcagc
     9541 tcatcagtcg cctgaccatt aaacatattt tttatggtgg cgttgtcgtg ctggcgttgt
     9601 tttacctgtt ctatctgttg cggctggaca gccagacgca ggacatcctg caccagctaa
     9661 acaaattact tcgataagga cgtttatgat ccagattgat cttcccacgc tggtaaaacg
     9721 gcttaacctg ttctcccgcc aggcgctgga gatggccgcc tctgaatgta tgagccagca
     9781 ggcagcggaa attaccgtca gccatgtgct tattcagatg ctcgccatgc cacgcagtga
     9841 cctgcgggtt attacccgcc agggcgatat tggcatggaa gagttgcgcc aggcgctgac
     9901 ggtggagaac tacacaactg cccgttctgc ggacagctac ccggcgtttt ccccgatgct
     9961 ggttgagtgg ctgaaagagg gctggctgct ggcgtcggct gagatgcagc acagcgaatt
    10021 gcgtggcggc gtgttactgc tggccctgct gcattcgccg ctgcgttata taccgcctgc
    10081 tgccgcccgg ctgttgaccg gcattaaccg tgaccgtctg caacaggact ttgtacagtg
    10141 gacacaggag tcggcggaat cagtcgtgcc ggatgcagac ggtaaaggcg caggcacact
    10201 gacggacgct gctgacaccc tgcttgcccg ctacgccaaa aacatgaccg cagacgcccg
    10261 taacggcagg cttgacccgg tactgtgccg cgaccacgaa atcaacctga tgatcgacat
    10321 tctctgccgc cgccgcaaga acaacccggt ggtggtgggc gaagcaggcg tgggtaaaag
    10381 tgccttaatc gaagggctgg cgctgcgcat cgtggcaggc caggtgccgg acaaactgaa
    10441 aaacaccgat atcatgaccc ttgacctggg cgcattgcag gccggggcgt cggtgaaggg
    10501 cgaattcgaa aaacgcttca aggggctgat ggcggaggtc atttcctctc cggtgccggt
    10561 cattctgttt attgacgaag cacataccct gattggcgcg ggcaaccagc agggcgggct
    10621 ggatatctcc aacctgctca aaccggcgct ggcgcgcggc gaactgaaaa ccatcgccgc
    10681 caccacctgg agcgaataca aaaaatactt cgagaaagat gccgctctgt cgcgccgctt
    10741 ccagttagtg aaggtcagcg aacccaacgc cgctgaagcc accattattc tgcgtggtct
    10801 gtcggcggtc tatgaacagt ctcacggcgt gctgattgat gatgacgcct tgcaggccgc
    10861 agcaacgctg agcgaacgtt atctctccgg gcgtcagttg ccggacaaag ccattgatgt
    10921 gctggatacc gcctgcgccc gcgtcgccat taacctttcc tcgccgccga agcaaatctc
    10981 ggcgctgacc actctgagcc accagcagga ggcggaaatt cgccagcttg agcgcgagct
    11041 tcgcatcggg ctgcgtaccg acacatcacg gatgaccgag gtgctggtgc agtatgatga
    11101 aacgctgacg gcgctggatg aactggaggc cgcctggcat cagcagcagg cgctggtaca
    11161 ggagattatc gcgctgcgcc agcaattgct gggcgtggca gaggacgatg cggcgccgtt
    11221 gccggacgca gataccgtgg aggatacgcc gccagagtca gagtcagagt cagagtcaga
    11281 gtcagaacag gataataccg gtgccgtacc ggctgatgag gccgacagag aacagccgga
    11341 agagaccgct gaaacagttt ccccggtaca gcggctggcc cagctcactg ccgaactgga
    11401 cgccctgcat aacgaccggt tgctggtctc cccgcacgtc gataaaaaac agattgcggc
    11461 ggtgattgcc gaatggaccg gcgtgccgct caaccgcctg tcgcagaatg aaatgtcggt
    11521 catcaccgac ctgccgaaat ggctgggcga caccatcaaa ggccaggacc tggcgattgc
    11581 cagcctgcat aagcatctac tgaccgcacg cgcagacctg cgtcgtccgg gacgcccgct
    11641 cggtgcgttc ctgctggctg gccccagcgg tgtgggtaaa accgaaaccg tcctgcaact
    11701 ggcagagctg ctctacggcg gtcgccagta cctgaccacc atcaatatgt ccgaattcca
    11761 ggagaaacac accgtctcgc ggctgattgg ttcgcctccg ggctacgttg gctacggtga
    11821 aggcggcgta ctgaccgaag cgattcgcca gaaaccctac tcggtagtac tgctcgatga
    11881 agtggaaaaa gcgcacccgg atgtgctcaa cctgttctac cagtcgttcg ataagggcga
    11941 aatggcagac ggtgaaggcc gcctgattga ctgcaaaaat atcgtcttct tcctgacgtc
    12001 caacctcggc taccaggtaa tagtcgagca cgccgatgac ccggaaacca tgcaggaaga
    12061 actgtatccg gtgctggccg acttcttcaa acctgccctg ctggcgcgta tggaagtggt
    12121 gccgtatctg ccgctgtcga aagagacgct cgccaccatt atcgccggaa aactggcccg
    12181 tctggataac gtgctgcgca gtcgctttgg tgcagaagtg gtcattgaac cggaagtgac
    12241 ggacgaaatc atgagccgcg tcacccgcgc ggaaaacggc gcgaggatgc tggaatcggt
    12301 catcgatggc gacatgctac cgccgctctc gctgctgctg ttgcagaaaa tggcggctaa
    12361 cacggcgatt gcccggattc ggttgtcggc ggtggacgac gcatttacgg cagacgtgga
    12421 agatgctcag aacgacgagt ccgtcacaaa ggatgaaacg gttttatgag caggttgcgc
    12481 gttttccggc agacaggatg gtttatcgcg ggcctgatga cgggcttacc cgccactgcc
    12541 gcgcccgcag aaacatcgtc aatggccggt gtggcggtcg ccgtcgccac cacgcccccg
    12601 ccggatgcga cagccacgtt acaggccatg cagtcctgtc ggcgggagtc cgcggcgctg
    12661 gagcggcttg actgctatga ccgcctgctg gcaccgctgt ccccgtcagg gtttgacggg
    12721 gcgctggtca aagccggttt tgtgggcgag gcctggaccc gcgccacaga gcaggaaaaa
    12781 cgccgcgagg gcaacaccac agaactgctg gtgacgcagg tgccggggga gcgcccgacg
    12841 gtggtgataa ccaccccggc tatcggccat gttccgccgc gcccggtgct gatgttcagt
    12901 tgcgtggaca atatcacccg gatgcaggtg gcgctgatgc accctctgga tgtccatgat
    12961 atcgccgtca ctctgaatgc cgacagccgg gcattgcgta gccactggtt tgtgcgcgag
    13021 aacggcaccc tgctggagtc cagccggggg ctgtccggga ttgatgaaat taagcaactg
    13081 ttcggcgcaa aaacgctgac agtggatacc ggcgcagata acgccgcagg aaagctgacc
    13141 tttaacattg acgggctggc aagggcgatt gcccccctgc gcgttgcctg ccactgggcg
    13201 ggagaataaa aatatggcaa tgaacttacg cgatccgaat gtctggatat cgcacctgct
    13261 ggaaaacctg ccggaagaaa aactggcatc ggcgctgaaa gatgacaacc cggactggga
    13321 gtatatcgac ggcgaaatcg tcaaactggg atctctggcc cacagtcaac tggatatccc
    13381 ggaactccag cgccgggggc tggtcattct ggcttcggag agtaaggact ttcgtctgct
    13441 ggcgcatctg ctgagaaccc tgcaacacgc cggtgatcct catctcgcat tgcgcctgct
    13501 ggcgctgtat gtggagcatt actggtcggt ggctgcgccg cagaatatgg cacataaaaa
    13561 acgctttgcc agccaggtca ttaaacgctt tgaaacaggt attgaaggct tttcacagaa
    13621 cgccgccaca gcgcagcacg acgcgctgtc aggggaactg gcgaaactgg cgcagtgctg
    13681 gcagtcatat aacgccccgg aactggcact ggctaccgat gatctttttg ccctgtacca
    13741 gcgtgcgttt aatcgtgcgg caccggctcc tgcccccact ccggcggcct ccgatagttc
    13801 acgacagacc accgccacgt ctgaaagcgg cgtgacgcaa cccagtgctc cggctcccca
    13861 aatcgccatc gacagtcacg acgacaaagc ctggcgcgac acgctgttaa aagtggcggc
    13921 tattttatgt gaacgccagc cggactcgcc gcagggctat cgcctgcgcc gccatgccct
    13981 gtggcaatcc atcaccagta caccccaggc ggaaagcgat ggacgtaccc cattggctac
    14041 ggtctctgcc gatatggtgg cggattacca gtcccggctt gccagcgcgg atatggcgct
    14101 gtggcagcag gttgagaaaa gcgtattgct ggctccttac tggctggacg gtcactgtct
    14161 ttctgcacag acggcactgc gtctgggtta caaacaggtg gcagacacca tccgcgatga
    14221 ggtcatccgc ttccttgagc gtctgcccca gcttaccggg ctgctgttta atgaccgcac
    14281 accgtttctc agtgagcaga cgaaacaatg gctggcagct tcgcccgacg gcaaagttgc
    14341 accggttgcg caaatcggtg aggaatcgca ggcagccaga gcctgttttg ctgggcaggg
    14401 tctggaggcg gcgctgcgat atctggacat gctacccgaa ggcgatcccc gcgatcagtt
    14461 tcaccgccag taccttgccg cacagttgac ggaggaggcg gggctgatac agcttgcgca
    14521 gcaacagtac cggatgttgt tgatgatagg gagtcagatg atggtgtctg actgggagcc
    14581 atcattactt acgcagcttg aacaaaaatt cacggcagaa caataaaaga gaacaggagt
    14641 ttttgtgttc agattaccaa caccccgact actcagcgga ctcaaatcag ccctacgccc
    14701 ggcgatgccc cggtttaaag tctccgcttt ctggctgctg atactggcgt ggatttttct
    14761 gctggtatgg atctggtgga aaggcccgac atggacgctg tatgaagaac agtggctcaa
    14821 accactggcg aaccgctggc tggcaacggc ggcatggggg attatcgccc tagtgtggct
    14881 taccgtccgg gtgatgaagc gcctgcaaca gcttgaaaaa atgcaaaagc agcagcgcga
    14941 ggaggccatt gatccgctca gtgtggagct gaacgcccag cagcgttacc ttgaccgctg
    15001 gctgctgcgc ctgcaacgcc atctcgacaa ccgccgtttc ctgtggcagt tgccgtggta
    15061 tatggtcatc ggcccggcgg gcagtggcaa aacgacactg ctgcgcgagg gttttccgtc
    15121 cgacattatt tatgccccgg agggcgcacg cggcgcagaa caacgcctgt acctcacgcc
    15181 ccatgtcggt aaacaggcgg tgatctttga tatcgacggc acactctgcg ctcccgctga
    15241 tgcggatatc ctgcatcgcc gtctgtggga acatgccctc ggctggctga aagaaaagcg
    15301 cgcgcgccag ccgctgaacg ggattattct gacactggat ttacccgatc tgcttaccgc
    15361 agacaaacgc cgccgcgagc atctgttaca gacgctgcgc agccgcttgc aggatatacg
    15421 ccagcatctt cactgccagt taccggttta cgtggtactc acccggcttg atttattgca
    15481 gggttttgcc gccctgttcc agtcactgaa cagacaggat cgcgatgcaa ttctaggggt
    15541 cacgttcacc cgccgcgccc atgaaaatga tgactggcga acagagttga atgctttctg
    15601 gcagacatgg gtggatcgaa tgaacctagc gttgccggat ctgatggtcg ctcagactca
    15661 cacccgcgcg tctttattta gtttttcccg ccagatgcag ggaagccgtg aaccgctggt
    15721 gtcactgctt gagggtctgc ttgacggcga aaatatgaac gtgatgctgc gtggtatcta
    15781 tctcacctct tcgcttcaac gcggacagat ggatgatatt ttcacccagt ctgccgcccg
    15841 ccagtaccgg ctgggcaata acccactggc gtcctggccc ctggtggaca ccgcgcctta
    15901 tttcacccgt ggcctgttcc cgcaggcatt actcgcagag cctaatctgg caacagaaag
    15961 ccgcacctgg ctgatgcgtt cccgtcgccg cctaacggtt ttctctacca caggaggcgt
    16021 ggcggcactg ctgctcatca ccggctggca tcactattac aacggtaact atcagtccgg
    16081 catcaccgtg cttaagcagg caaaagcctt tatggatgta ccgcctccgc tgggggaaga
    16141 tgattacggc aacctgcaac tgccgctgct gaacccggta cgcgatgcca cgctggccta
    16201 tggcgactgg ggcgaccgca gccgtctggc cgatatggga ctgtaccagg gacggcgtat
    16261 cgggccttat gtggaacaaa cctatctgca actgctggag cagcgttacc tgccttcgct
    16321 gtttaacggg ctggtcaaag cgatgaacgc cgcgccgccg gagagtgagg aaaaactcgc
    16381 cgtgctgcgt gtgatgcgaa tgctggagga caaaagcgga cgcaataatg aggtggtgaa
    16441 gcagtatatg gcaaaacgct ggagcgaaaa attccacggc cagcgcgata tccaggcaca
    16501 actgatgtcc catcttgact acgcgctggc tcatactgac tggcatgcag agcgtcaggc
    16561 gggcgacggt gacgccatca gccgctggac gccatatgac aagctcgtgg tatcagcaca
    16621 gaaagaactg agcaaactgc ctgtctacca gcgggtttat cagagcctga aaacgcgggc
    16681 gctgggcgtt cttcctgctg acctcaatct gcgtgaccag gtagggccaa cctttgacca
    16741 ggtgtttaca tctgccgatg acaacaaact ggttgttcca cagtttatca cccgctatgg
    16801 cctgcaaagc tattttgtaa aacagcgcga tgaactggtt gaactgacag cgatggattc
    16861 ctgggtactt aacctcaccc gcagtgtgaa atacagtgac gccgaccgtg cggaaatcca
    16921 gcaccagttg accgagcagt atatcagcga ctacaccgcc acctggcggg ccgggatgga
    16981 caatctgaat atccgcaatt ttgagtccat cggacaactg accggggcgc tggagcaggt
    17041 tatcagcggc gaccagcctt tgcagcgggc gctgaccgtg ctgcgtgaca acacacagcc
    17101 aggcgtcttt tctgaaaaac tctctgccaa agaacgggag gaagccctgg cagagccgga
    17161 ttaccagtta ctcacccgcc tcgggcatga attcgccccg gaaaacagta ccctggcagt
    17221 acagaaagac aaagaaagca cgatgcaggc cgtgtatcag caactcaccg agttgcaccg
    17281 ctacctgctg gcaatccaga acgcgcctgt accagggaaa tcggcgctga aagccgtgca
    17341 gttacggctt gatcagaaca gcagcgatcc gatattcgcc acccgccaga tggcaaaaac
    17401 gctgcctgct ccgctcaacc gctgggttgg cagactgact gaccaggcct ggcatgtggt
    17461 gatggtggag gctgttcatt atatggaagt ggactggcgc gacagcgtgg tgaaaccgtt
    17521 taacgagcaa ctggcaaata actatccgtt taatccgcgt tctgcacagg atgcctcact
    17581 ggatgccttc gaacgcttct ttaaaccgga tggcatactg gataccttct accagcagaa
    17641 cctgaagctg tttatcgata atgacctgag tctggaggat ggcgataaca acgtcattat
    17701 tcgcgaagat attattgcgc aactggaaac tgcgcagaaa atccgtgaca tcttcttcag
    17761 caaacagaac ggtctgggaa catcctttgc cgtggaaacg gtatcgcttt ccggcaataa
    17821 acgccgcagt gtactgaacc ttgacggtca gttagtcgat tacagccagg gccgtaacta
    17881 taccgcccat ctggtctggc ctaacaacat gcgcgaaggc aacgaaagta agctgacgct
    17941 catcggcacc agcggcaacg cgccgcgcag tatcagcttc agcgggccgt gggcgcagtt
    18001 ccgcctgttc ggggccggac aactgaccgg agtacaggat ggcaacttta ccgtgcgctt
    18061 tagcgtggac ggtggcgcga tgacctaccg tgtgcatacc gacaccgagg ataacccgtt
    18121 cagcggtggg ttgttcagcc agtttggtct gtcagacaca ctgtactgag gaggcaggga
    18181 tgaatagtaa cgtactgaca caaactatcg ttaccggcag tgacccgcgc gggctgccgg
    18241 aattcagcgc cattcgcgag gaaataaaca aagccagcca cccgtcacag cctgagctga
    18301 actggaaact ggtggagtcg ctggcgctgg cgatttttaa agctaacggt gtggatttac
    18361 acaccgccac ctactatacg cttgcacgga cacggacaca gggactggcg ggcttttgcg
    18421 aaggtgcgga actgctggcg gcaatggtaa gccacgactg ggataagttc tggccacagg
    18481 gcggtccggc gcgtactgaa atgctggact ggtttaactc ccgaaccggc aatattctgc
    18541 gtcagcaaat ctcctttgcg gaatccgacc taccactgat ctaccgcaca gagcgggcat
    18601 tgcagcttat ctgcgacaag ctccagcagg tagagctgaa gcgcgttccg cgcgtggaga
    18661 atctactcta ttttatgcag aacacgcgta aacggcttga accccagccg aagagtaacg
    18721 cagagaacgc cgcacagacc acggtcagaa cgctgattta tgccccggaa acaatggctt
    18781 cctccacgcc agaagcggta gtgcctcccc tgcccgacct gcctgagatg aaagtggaag
    18841 tgcgcagtct gtcagaaaat cccccacagg ccagtgtgat gaagcaaggc agtacggtaa
    18901 gagggtttat cgcagggatc gtctgttcag tggctatcgc ctcagcattg tggtggtggc
    18961 aggtctatcc ggcgcagcag caactgttac aggttaacga caccgcgcag ggcgcagcaa
    19021 cggtgtggat ggcctcacct gaacttgaaa actatgagcg caggctgcat cagcttcttg
    19081 gcgtctcccc ggtacaaccg ctggaaaccg ggatgcagat gatgcgtgtt gccgacagtc
    19141 gctggccgga aagcctgcaa cagcagcagg cctcgacaca atggaatgag gcactcaaaa
    19201 cccgcgcaca gagtagccca cagttgcgcg gctggttgca gacccgccag gacttacatg
    19261 cttttgctga tctcgtgatg cagcgcgaga aagagggatt aaccctttcc tatatcaaaa
    19321 atgtcatctg gcaggcggag cggggactgg ggcaggaaac acctctggaa tccctgctga
    19381 cgcagtacca ggatgcccgt gcgcagaagc agaatacaga tgcgctggaa aaacagatta
    19441 acgagcggat ggacggcgtg ttaagtcgct ggctgctgct gaagaataat acgataccga
    19501 cgataaaaaa agacattgaa tttcaataac attcatgaat acaaaggagt tttaaatggc
    19561 gaatttaatt tatttaacac taaatggtga taatcaaggg ctcatttcaa gtggttgttc
    19621 atcacaacca tccattggaa ataaagctca aacagcacac attgatcaaa ttatgattta
    19681 tgaattcatg catggattaa atcgagatca aaacgtcaac catcatcatt tgacaataaa
    19741 aaaacctata gataaagcat cacccttatt aggcaaagca atttgcgaca atgaattatt
    19801 gacatgtgac ttttcctttt atcgtacaaa taaatttgga attaatgaac tgtactataa
    19861 aatcaaatta actggagcaa agataagcga cattcatgtc tctatatcac atattgttgt
    19921 tgataacagc attcaacccg aagagtcagt ttctttcagt tatgagtcaa ttatatggga
    19981 gcattgttcc gctggcacta gtgcatacag tttatgggaa gatcgtcttt tttaaaaata
    20041 tctgggatta ttataatggc taacaatatt ttacactctt caacatcaga cagtgaaagc
    20101 aaaaatacaa gcactgacag tacgttattt aatgaaaaat tcccatttca actaaggcaa
    20161 gatttcgcat caagaataac agaagatgaa agtgattttt ttaaatggaa aaaaataaga
    20221 gagttggcat ttgtgagcaa tcataaagaa tggaataaat atgacttatt aattttaaaa
    20281 tctgttaatg aaatcaacat tcatttgtcc agcactccat actttcagcc gttggattgg
    20341 tatataataa aagccatgct ttggactgaa aacgatgcgg caaatacttc tcagtggaat
    20401 ggttatccat tacaaattgg tcgttttcgg aaagataaag cgatgcctgc tttaatctct
    20461 ggagaaaaat ccgcagccct tgttacccca ccacaatgga gaaacaaagc attcaatggg
    20521 ttaaaagatc cagaaagaaa ttactgggca aaagaacaaa taactggctc tccagaagag
    20581 aatatcaaag cagcaattac ttacttaatg atgaaacttt ctaatacaaa ggaagaatca
    20641 actatcgatc aatatgactc aactctatat agcgtcatcg tccaaaaagg cgatttggct
    20701 gacaacatca gaaaagagag aaaaacgacc atacctaact taacgaaaaa caaccctgga
    20761 aaaaatctcg ataaaataca cccgggagat attttatatt atcaaaaagc atccatgaaa
    20821 gtaataatta ctggatggaa acctatcaca ataaaaaatg tagcgatgaa ttataatggt
    20881 ggtggagatc ccaagtatgc aataaaactt cagttcgttt atacattgct cacaaaaaac
    20941 agagtgttat gagaatggga aaaataacaa ttattctact gcttacattc tcatgtacaa
    21001 aatgtctggc atttgacatt tccatgtcta aagaatgtag tggcgttgat gaacttgaat
    21061 ttgttgattg tgttaaaaat tcatttatta tgagcactat tacacttaaa aaagcagaag
    21121 taaatataaa agagaaaatt aaaaaatgga ataacgatga ggacgaagca ataagaaaag
    21181 aagcagttat ttctttaaaa agactagaat cttctaatcg ggaatttata cgttacagat
    21241 ttgctcaatg ctcttttgct ctaacctggg gggccagagt aggacgtctg gcattctctc
    21301 gactatatcc atgctatact gagttaaaca aaataagagc catgcaacta aacaatgctt
    21361 acatttcaga ttttcaaaat ccagatgaga tatccagcaa atgattatta taaaattaac
    21421 attactattt atactggcca caattacata ttcagtacaa tcagacgaat tcgatgatca
    21481 aaccacttac acgcaatacc gttatttttc cacagccata ccagaagata tacgtagatt
    21541 tatgttctac gtagattatt gctttgtaga tttagcattc gatatcgaaa ctgactcaat
    21601 gacagctaaa aatgatatta ttaatgaatg taccccatta aaagaaagaa acaaaacatt
    21661 aaaagaaaaa tataaaaaag atgaagagat aatagcaatt ctttccattg cagatgatac
    21721 cattttaatt tatgaagaat taaaaaaata actcacatgc aattcactgg caatatatat
    21781 tataatttta taaaaaaaca tatttacatt atagaaatac tcagctcctg tttgctgccc
    21841 gccctaaact acaggcggca gcataacaat aacgattact ggaatcgact caccaagtct
    21901 ttcaccatcg atacatatgt tttttgtttc aaattaaaca gttgcacttc cgtaacatgg
    21961 tcatttgata gttctttatt gctcattttt gttattccag ccggcggaaa aattactctc
    22021 gattcataat tcaccggcga ccaggtagcc cactcacctg ttgccacatc caccagcgtg
    22081 aaacgtacca ggtagcgcag ggagatagcg tcagtgagtt tttcattttt atagtcggac
    22141 catactgttg atttcagcgc agagtcatat tttcctgtct gcaacgtatc atggtaaaca
    22201 ataatcgctt tctgatgccc tttcgcagcc acaaaacgca atgcctgcat ccagttcata
    22261 ttttcagcac tggcaacatc ctcgttctca tttttatctt tatttttgtt acaagtcaga
    22321 ggcttttgcc tgtccgggat accagagaat gtggaaacag tataatattt tctcatctcc
    22381 tcctgcataa tggtttccgg tgcgcgactg ccagactgga caagtattac gggagaattg
    22441 agaggcacac gaaactcatc tccttcaagc gcagcctgaa tatcctcctc ggatacagcc
    22501 agagtggttt cattaccgaa taaatctttg tctgtcagtt gcgggggaat gtttttatta
    22561 atattacttt gtgtaacttg agcttttttc tttttttcat caatagaaga ttgagaggca
    22621 catcctgcaa gcagcgagaa taatacacat atgcaacaaa ccttttttga attcataatc
    22681 ggacactccc tcgcctgtca taatgattag aatatttagc actgataacg gactctaatt
    22741 atatacccca cttaaataat aacaaacaaa atcaacatga aatatacatt catttaatca
    22801 atataaaatt atcctctaaa aatatattta agttgaatgg ttcgatatca ttattgaatt
    22861 ttaataaatg agacaacaag cgagagttac gcacttacgc cagagtaaaa tcattgaaca
    22921 gagaaatggc gggattcaaa tacaaaaaaa ccgcaaaaat aaaaattttt gcggttctct
    22981 gaactcattt tcatgagtga atttggcgga acggacggga ctcgaacccg cgaccccctg
    23041 cgtgacaggc aggtattcta accgactgaa ctaccgctcc gcgttttgtt ccgttgggaa
    23101 cgaggcgaat attacggatt gcctcaccct tcgtcaacgg tttttctcat cttttgaatc
    23161 gtttgctgca aaaatcgccc aagtcgctat ttttagcgcc ttttacaggt atttatgccc
    23221 gccagaggca gcttcccccc ttcttctgca ccagatcgag acgggcttca tgtgctgcaa
    23281 gctcttcatc tgtcgcaaaa acaacgcgta acttacttgc ctgacgcaca atgcgctgaa
    23341 ttgttgtttc atcttgttgc tgttgtgtct ctccttccat cgcaaaagcc atcgacgttt
    23401 gcccaccagt catcgccaga taaacttccg caaggatctg ggcatcgagt aatgccccgt
    23461 gcagcgttcg tttactgtta tctatttcgt agcgggcaca taacgcatcg aggctgttgc
    23521 gcttaccggg aaacattttc ctcgccaccg caaggctatc ggtgacctta cagaaggtat
    23581 tggtcttcgg aatatcgcgc ttaagcaacg aaaactcgta gtccataaag ccgatatcga
    23641 acgctgcgtt atggatcacc agctccgcgc cgcggatata gtccatgaac tcatcggcta
    23701 cctctgcaaa cgtgggctta tcgagcaaaa attcatcggc aataccatgt acgccaaagg
    23761 cttccggatc caccagccga tcgggtttga gataaacatg gaagttattg cccgtcaggc
    23821 gacggttcac cacttcaacg gcaccaatct caatgatctt gtggccttca tagtgcgcac
    23881 caatctggtt cataccggta gtttcggtat cgagaacgat ctggcgtgta attgcagtgc
    23941 tcatagcggt catttatgtc agacttgtcg ttttacagtt cgattcaatt acaggaagtc
    24001 taccagagat gcttaaacag gtagaaattt tcaccgatgg ttcgtgtctg ggtaatcctg
    24061 gacctggggg ttacggcgct attttacgct atcgcggacg cgagaaaacc tttagcgcag
    24121 gctacacccg caccaccaac aaccgtatgg agttgatggc cgctattgtc gcgctggagg
    24181 cgttaaaaga acattgcgaa gtcattttga gtaccgacag ccagtatgtc cgccagggta
    24241 tcacccagtg gatccataac tggaaaaaac gtggctggaa aacagcagac aaaaaaccag
    24301 taaaaaatgt cgatctctgg caacgtcttg atgctgcgct ggggcagcat caaatcaaat
    24361 gggaatgggt taaaggccat gccggacacc cggaaaacga acgttgcgat gaactggctc
    24421 gtgccgcagc gatgaatccc acactggaag atacgggcta tcaagtcgaa gtttaagcct
    24481 gtggtttacg acattgccgg gtggctccaa ccgcctggcg aattcgtggc ttgtttttac
    24541 tctgtttcat cggatttagc gttaaaggaa tggtccgttt ccgggcaaca ataagttgta
    24601 agcaaccaag cgcaggaata tgcgcattca atagttttcc tccgtgtttg ttccacggga
    24661 gaacgtggaa acggctggcg tgtagcactt caaaattcaa caaagagagc cagtccagct
    24721 gccgcatcag agtaaacatc cggctgttat agggcgaggt tttgcgcaat accggcacaa
    24781 gtttgcgtaa tcccattaaa ctgatgggat tgaagccact aatcaccagc cagccatcat
    24841 caatcaatac ccgatcggct tcacgcaata aacgatgcgg atcggtgcac cacggcaatg
    24901 tatgtgccag tagacaaaca tcaacggatt tatcggcaaa aggaagatga agtgggtccg
    24961 cctgcacctg gacgggcagt ccttgcgcag aaacattcac ttgatgagaa accgcgcacg
    25021 cttcgcaatt gatttctgcg cttaaattgc caatcttaag cagatgaaaa ccatacattt
    25081 tagtgaacca cgggttgagc tggcgctcca gcgccttgcg ataaagctct ccccagggca
    25141 aatcgcccca gcaatcagga gccacgacag tttgagggac tcttgccggt ttcatcacaa
    25201 ccttccgttt cacactgaga ggtaatctat gaatcttaac agtattcccg cctttgatga
    25261 caattacatc tgggttttga atgatgaagc aggtcgctgc ctgattgtcg atcccggaga
    25321 cgcagagcca gtattaaacg ccattgccgc caataactgg caaccggagg ccatatttct
    25381 cacccaccat caccacgatc acgttggcgg cgtaaaagaa ctggtgaaaa agtttccgca
    25441 aattgtggtg tatggtccac aagagacaca agataaggga acaacacagg tagtcaaaga
    25501 tggcgaaact gccttcgttt tggggcatga atttagtgta attgccacgc cgggtcacac
    25561 tttaggacat atctgttact tcagtaaacc ttatctattt tgcggcgaca cgctgttttc
    25621 tggtgggtgt ggtcggttgt ttgaagggac agcatcacaa atgtatcaat cacttaaaaa
    25681 gttaagtgcg ttacctgacg atacattggt atgttgtgct catgaatata ccttatcaaa
    25741 tatgaagttt gctttgagta ttcttccgca cgatttgtcc ataaatgatt attatcgtaa
    25801 agttaaggag ttacgggcaa aaaatcaaat aacactaccc gtaattctga aaaatgagcg
    25861 gcaaattaat gtttttttaa gaacagaaga tattgattta attaatgtaa ttaatgaaga
    25921 aacattattg caacaacctg aagaacgttt tgcatggtta aggtcaaaga aagataggtt
    25981 ctgataaaac tttcttgtca tcggctccgt tcgccgttat gatcggtcgt cttttaagca
    26041 actattgaca cacacatgaa ggcaaaagcg atattactcg cctctgtcct gctcgtgggt
    26101 tgccagagta ccggcaacgt tcaacagcac gcacagagcc tttctgcagc tggtcaaggg
    26161 gaagcagcaa agtttacaag tcaggcacga tggatggacg atgggacgtc tatcgcgcca
    26221 gatggtgact tgtgggcttt cattggcgac gagctaaaga tgggaattcc ggaaaatgac
    26281 cggattcgcg aacagaaaca gaaatattta cgcaataaga gctatctcca cgatgtaact
    26341 ttacgggcag agccgtatat gtactggata gccgggcaag ttaaaaaacg taacatgcct
    26401 atggaactgg tactactacc catagtggag agcgcttttg atcctcacgc aacgtctggc
    26461 gccaatgccg caggcatctg gcagatcatt ccgagcacgg ggcgcaatta tggtttgaaa
    26521 cagacccgca attatgacgc gcgtcgcgat gttgttgctt caacaactgc cgcgctgaac
    26581 atgatgcagc gtctgaacaa aatgtttgac ggcgactggc ttctgaccgt agctgcttat
    26641 aacagcggcg aaggtcgagt catgaaggca attaaaacga acaaagcgcg tgggaaatcc
    26701 acggacttct ggtcgttacc gttgccgcag gaaacgaagc agtacgtgcc taagatgctg
    26761 gcattgagtg atattctcaa aaacagcaag cgttatggcg tacgtctgcc aacgaccgat
    26821 gaaagccgtg ctctggcacg tgtgcacctg agcagcccgg ttgaaatggc gaaggttgca
    26881 gatatggcgg ggatttccgt cagcaagctg aagacattca acgcaggcgt gaaaggctcc
    26941 acgctgggcg caagtggtcc gcagtacgtg atggtgccaa agaagcatgc agatcaactg
    27001 cgtgaatctc tggcttcagg cgaaattgct gctgtacagt cgacgctggt tgccgacaat
    27061 acgccgctta acagccgtgt ttacaccgta cgctctggcg acacgctttc aagtatcgct
    27121 tcacgtctcg gcgtaagcac caaagatttg cagcagtgga acaaactgcg cggatctaag
    27181 ctgaagccag gccaaagttt gacgattggt gcaggcagta gcgcacagcg actggcaaac
    27241 aacagcgata gcattacgta tcgtgtgcgc aaaggcgatt cgctttcaag cattgctaaa
    27301 cgccacggcg tgaacatcaa agatgtaatg cgctggaaca gcgacactgc gaatctgcaa
    27361 ccaggcgata agctgacgtt gtttgtgaaa aacaacaaca tgccagattc ctgacaaacc
    27421 agataataaa aggcaccgat tcccccggtg ccttttttat ttatgccgct ttatgtgctt
    27481 ctaccatgat gatatcactg gtgaaagagc catcattctg caaggcaaaa tacgttttca
    27541 cctctgtcga tgcgctctgt tggtaaatgc gaatagcgtc tactaacgct tctggcgtac
    27601 gcattctcgc gacccatgaa gaaaattcca gcggtaactt atctgtaatt aaattatcaa
    27661 ctatcagatt ggcttcattg attaacgtca accactcacc gctggcgtag tttcgtacgt
    27721 gagaggtatc gcgtaatgct tctaccgtct gtaaccagac gtcgcgcact gggtgacccg
    27781 gagacattac gtccatcaca atcagcctac cgccaggttt caatatccta ttcacttctc
    27841 gcagtgctgc accaacatca tgccagtgat gggcagaata acggctgata acaatatcaa
    27901 atgcgttatc ggcaaatggc agactttcgg catatccctg gcgggtggcg atatttttca
    27961 ggtgccgggc ttcggcagct tgtgccacga catccagcat ttgggcagat aagtcatacg
    28021 ccaccaccgc gctcacgttc tgcgcagcga caaagctggc atgccctgct ccgcagccca
    28081 tatcaagtac acttgcatca ggataatcag ccagacgcac cgccaggcgc tgtaaatctc
    28141 gcccggatgc atgtacggta ctggttaaat actcactggc ctgcgagcta aactgctttt
    28201 ctacgtggtc atggtgggat tgtgttgtca ttgcgctgtc cttttattgt ttgattaaaa
    28261 agggcagcgc caaacgttgg tctgccctgt ggcagacctg acatacctta tttatcaggc
    28321 ttgccgggac tgaattcaac gagtagcgga ttgtgatcgg aagcgcgcgt aaccagtaca
    28381 gaagcttcgc tgacgttcag accacggtag aaaacaaaat cgagcgggcg gccaaacgcc
    28441 cgacggcgct gatcatcggt aaaacgcacc tggcgcagcg acatttcccg cgcaaagcga
    28501 tataacgcgt tcatccttct acggctccag gcattgaaat ctcccgccat aatgaccggg
    28561 ccgctgtggt gagctatctg atcgccaata ggaagtaact gcttactata gacatccacg
    28621 cccaaactga aattgacggc gtgtatatta accaccatca acaggcgggt gtcaggcaat
    28681 ggatagaccg tcaccagtgc cgacttcgcc agacgcaaaa tgggttctcg ttcacgtaac
    28741 gggcagcaat acactggatg cgccgccgaa agggtcatta caccggaagg atgttgtggc
    28801 agcacgaaag cgggtacctg atcggcggca agatagttag cggtcgcaaa ctgcactaac
    28861 tctggcgttg tctgcgcttc ctgcaataac accagatgtg catctttgcc gtagttcttt
    28921 aataccgaca accattcagc gcgttgctgt ttgtatatgt tccacaccag gatccgaata
    28981 cgctcttcgg tacttaacgg ttctccaggt ggtaatgcct ggccgatgct cgcaaaagac
    29041 cccggcggta agatcctttc cgcaggttgt ccagcaacat agcgcatggc ataggtgttt
    29101 tttcgcactt ttgactttga aacctctatt acgtcaaccc ttccgcactc gggagggttc
    29161 tactgttata gggattttag ctcactcttt caacgagcaa ttgtatattg ctatgtaagc
    29221 aagtgcttac accggtcaga aatttgctca ttattgagaa cattaaatcg gatgacatat
    29281 ctcgcatcct gatggtttaa tgacaattaa attcaaaata tctcttctat tttgcaacag
    29341 gagcaaaaat gaaagccacg tcggaagaac tcgccatttt tgtttcagtc gtcgaaagcg
    29401 gcagctttag ccgggcagcg gaacaattag ggcaagcaaa ctcagcggta agccgggcgg
    29461 tgaaaaagct ggagatgaaa cttggcgtta gcctgcttaa tcggaccacg cggcaactta
    29521 gcctgacgga agaaggcgag cgttattttc gtcgcgtaca gtcaattttg caggagatgg
    29581 cagcggcaga atcagaaatt atggagacgc gtaatacacc gcgtggactg ttacggatcg
    29641 atgccgcaac tccagtggtg ctgcactttc tgatgccgtt gattaagcct tttcgtgaac
    29701 gctatccgga agtcacttta tcgctagtct cctccgaaac gattattaat ttgatcgaaa
    29761 gaaaagtgga tgtcgcgata cgcgctggta cgttaacgga ttccagctta cgtgccaggc
    29821 cgttatttaa cagttatcga aaaattatcg cctcccccga ttatatttcc cgctacggga
    29881 agccagaaac gatcgacgat ttaaagcaac atgtttgcct gggattcact gaacccgctt
    29941 ccctcaatac ctggccgata gcctgtagtg atggacaatt acatgaggtg aagtacggtt
    30001 tgtcatccaa tagtggggaa acactgaaac agctttgcct gagtggcaac gggattgcgt
    30061 gtttgtccga ctacatgatc gacagagaaa tcgctcgcgg agaattggtg gagttaatgg
    30121 cagataaagt attgccagtg gaaatgccat tcagtgccgt atattacagc gaccgtgcgg
    30181 taagtacgcg catccgggct tttatcgatt tccttagcga gcatgtaaaa acagctcccg
    30241 gaggagctgt cagagaggct taatcccatt caggagccag accttccggg ctaaccaggc
    30301 ggtcgttgca atccagtgcg gcgatcgctt ttttatcttc ggcatcaagc tgtaaatttt
    30361 gtgccttaag attactttcc aggtttttac gtttagtaga agaaggaatt acggagtaac
    30421 cttcccccat agcccacgcc agaatcactt gtgccggagt cgcattgtgt ttagctgcga
    30481 tacgagcaat aacctcatct ttcagggcct taccatacgc cagcgtcata taggaagtaa
    30541 tatggatgcc gtgctgttta gcccaggcga ccactttacg gttttgcaga taaggagaga
    30601 gttcaatctg gttagtagcg atgttttcag cgccaacagc agcaatcgcc ttttccatca
    30661 atgggatcgt gaagttggaa ataccgatct cacgcgtcag cccttgtttt ttggcttcca
    30721 gcagcgcctg cataaactct tcaacagaga cttcatcgtt tggtgacggc cagtggatta
    30781 gcgtcagatc aacataatcg gtacgcaatt tttgcaggct ctctttcaga ctcgggatca
    30841 atttgtcttt gctgagattt tcaatccaga ttttagtggt gatgtagagt tcatgacgtg
    30901 gcacgccact ttctgcaatc gcctgaccta ctgcggcttc gttatcatag atttgtgcgg
    30961 tatcaattgc gcgataacca agttcaagcg ccgttttcac agatgaaata acaacgtcgt
    31021 ctttcagacg gaaagtacct aaaccaaatg cagggatagc cataatatgc ctcttttaat
    31081 gctattttcg ttaactgagg tgattatggg cttcagactg ttgcggaaaa agagcgtaaa
    31141 acgcagagga tttttgcgat tctggcaata atagaaatac agacgaaaaa aacctcgagc
    31201 gttaactcga ggcttcttaa taagtggcgg aacggacggg actcgaaccc gcgaccccct
    31261 gcgtgacagg caggtattct aaccgactga actaccgctc caccgaatt
//
LOCUS       DAZGQL010000062        30991 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.062, whole genome shotgun sequence.
ACCESSION   DAZGQL010000062 DAZGQL010000000
VERSION     DAZGQL010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30991
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.062"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..879
                     /gene="ybdO"
                     /locus_tag="ACHKIR_003090"
     CDS             <1..879
                     /gene="ybdO"
                     /locus_tag="ACHKIR_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO9990789.1"
                     /translation="KFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQSLQRLRAQF
                     NDPLFIRSGKGIAPTTTGLNLHHHLEKNLRGLEQTINIVNKSELKKNFIIYGPQLISC
                     TNNNMLIRCLRQDTSIDIECHDILLSAENAEELLVQRKTDLAITLQPVISRSVICMPL
                     HTIRNSLICSNKHPRITDTSNYEQIIAEEFTLLISKSAGIDEIQMDIDERFINRKVSF
                     RSSSLLTIINSISVTDLLGIVPTELYDLHRDFLKLKEIKLEQPLPAVKLYISYNKASL
                     NNLVFSRFIDRLNDSF"
     gene            1026..2246
                     /gene="ybdN"
                     /locus_tag="ACHKIR_003091"
     CDS             1026..2246
                     /gene="ybdN"
                     /locus_tag="ACHKIR_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFO9990790.1"
                     /translation="MSIYKIPLPLNILEAAKERITWTLNTLPRICVSFSGGKDSGLML
                     HLTAEIARQMGKKICVLFIDWEAQFSCTINYVQSLREFYADVIEEFYWVALPLTTQNS
                     LSQYQPEWQCWEPDVEWVRQPPQDAITDPDFFSFYQPGMTFEQFVREFAEWFSQKRPA
                     AMMIGIRADESYNRFVAIASLNKQRFADDMPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YANHQQLCNPLYNIMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHW
                     YQKKGIEVPQTQQGDIGAKDIPSWRRICKVLLNNDYWCRALSFSPTKAKNYQRYNERI
                     KGKRQEWGILCNND"
     gene            2219..2848
                     /gene="ybdM"
                     /locus_tag="ACHKIR_003092"
     CDS             2219..2848
                     /gene="ybdM"
                     /locus_tag="ACHKIR_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFO9990791.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLIPNDYNPNNVAPPEKKLLKKSIETDGFTQPIVVTHTDKNALEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(2849..4009)
                     /gene="ybdL"
                     /locus_tag="ACHKIR_003093"
     CDS             complement(2849..4009)
                     /gene="ybdL"
                     /locus_tag="ACHKIR_003093"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HFO9990792.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTRMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRIALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALSESRLEILPCEGTYFLLVDYSAVSSLDD
                     VKFCQWLTREHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            4118..5206
                     /gene="hcxA"
                     /locus_tag="ACHKIR_003094"
     CDS             4118..5206
                     /gene="hcxA"
                     /locus_tag="ACHKIR_003094"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HFO9990793.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHDFFTDEQLSRAVWIYGER
                     AIAAAQTKLPPAFELPGVKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAVPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQEYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALS
                     DQQKQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTEAYQRFHLPTTLAELEVDINNQVEIDKMIAHTLRP
                     VESIHYLPVTLTPDTLRAAFENVESFKA"
     gene            complement(5216..5413)
                     /gene="ybdD"
                     /locus_tag="ACHKIR_003095"
     CDS             complement(5216..5413)
                     /gene="ybdD"
                     /locus_tag="ACHKIR_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFO9990794.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(5526..7631)
                     /gene="cstA"
                     /locus_tag="ACHKIR_003096"
     CDS             complement(5526..7631)
                     /gene="cstA"
                     /locus_tag="ACHKIR_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter CstA"
                     /protein_id="HFO9990795.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLALFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFAITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALVALKEPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(7812..8225)
                     /gene="entH"
                     /locus_tag="ACHKIR_003097"
     CDS             complement(7812..8225)
                     /gene="entH"
                     /locus_tag="ACHKIR_003097"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HFO9990796.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(8228..8974)
                     /gene="entA"
                     /locus_tag="ACHKIR_003098"
     CDS             complement(8228..8974)
                     /gene="entA"
                     /locus_tag="ACHKIR_003098"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HFO9990797.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFTQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDVLVNAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(8974..9831)
                     /gene="entB"
                     /locus_tag="ACHKIR_003099"
     CDS             complement(8974..9831)
                     /gene="entB"
                     /locus_tag="ACHKIR_003099"
                     /EC_number="3.3.2.1"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HFO9990798.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDSLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPIPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(9845..11455)
                     /gene="entE"
                     /locus_tag="ACHKIR_003100"
     CDS             complement(9845..11455)
                     /gene="entE"
                     /locus_tag="ACHKIR_003100"
                     /EC_number="2.5.1.-"
                     /EC_number="6.2.1.71"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HFO9990799.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLLNDSGE
                     HNLQDAINHPAEDFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIEKHQVNVT
                     ALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(11465..12640)
                     /gene="entC"
                     /locus_tag="ACHKIR_003101"
     CDS             complement(11465..12640)
                     /gene="entC"
                     /locus_tag="ACHKIR_003101"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HFO9990800.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFAGAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QASARRFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRKRSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            12829..13785
                     /gene="fepB"
                     /locus_tag="ACHKIR_003102"
     CDS             12829..13785
                     /gene="fepB"
                     /locus_tag="ACHKIR_003102"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HFO9990801.1"
                     /translation="MRLVPLYRNALLLTGLLLSGIAVVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAETVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLASGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            complement(13789..15039)
                     /gene="entS"
                     /locus_tag="ACHKIR_003103"
     CDS             complement(13789..15039)
                     /gene="entS"
                     /locus_tag="ACHKIR_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042931 - enterobactin transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042930 - enterobactin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HFO9990802.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QIMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDS"
     gene            15150..16154
                     /gene="fepD"
                     /locus_tag="ACHKIR_003104"
     CDS             15150..16154
                     /gene="fepD"
                     /locus_tag="ACHKIR_003104"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HFO9990803.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAL
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLYTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            16151..17143
                     /gene="fepG"
                     /locus_tag="ACHKIR_003105"
     CDS             16151..17143
                     /gene="fepG"
                     /locus_tag="ACHKIR_003105"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HFO9990804.1"
                     /translation="MIYVSRRLIITCLLLVSACVVAGIWGLRSGAITLETSQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMAGGIVTSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAASLLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLVADLCAQLLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            17140..17955
                     /gene="fepC"
                     /locus_tag="ACHKIR_003106"
     CDS             17140..17955
                     /gene="fepC"
                     /locus_tag="ACHKIR_003106"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HFO9990805.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEDAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTTPSTAKI"
     gene            complement(17952..19085)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHKIR_003107"
     CDS             complement(17952..19085)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHKIR_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
                     /protein_id="HFO9990806.1"
                     /translation="MSSLNIKQGSEAHFPEYPLASPSNNEVDLLNLIEVLWRAKKTVM
                     AVVFAFACAGLLISFILPQKWTSAAVVTPPEPVQWQALEKTFTKLRVLDLDIKIDRTE
                     AFNLFIKKFQSVSLLEEYLRSTPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDESALYTSWTLSFTAPTSEEAQKVLAGYIDYISALVVKESIENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKTNVNDVNFTPFKYQLRPSLPVKKD
                     GPGKAIIVILSALIGGIVACGGVLLRHAMASRKQDAIMADHLV"
     gene            complement(19301..23182)
                     /gene="entF"
                     /locus_tag="ACHKIR_003108"
     CDS             complement(19301..23182)
                     /gene="entF"
                     /locus_tag="ACHKIR_003108"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031177 - phosphopantetheine binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HFO9990807.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGEVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALTFELPETIDLRTNIDPHGTAQSLMQ
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCAW
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLPGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALTALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPQLATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGEEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYAEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPHAPALADARYLFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     ADVPDLTSLCYNAPLTPQGSAPLKLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQNCATLKQVFCSGEALPADLCREWQQLTSV
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKAQAPGRAP
                     KAGSETIIAAAFSSLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFVRQVTPGQVM
                     VASTVAKLATIIDGEEDSTQRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DSQWSIIGIQSPRPHGPMQTAANLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERALQEGMNPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFEKIGPIIRATLNR"
     gene            complement(23179..23397)
                     /gene="ybdZ"
                     /locus_tag="ACHKIR_003109"
     CDS             complement(23179..23397)
                     /gene="ybdZ"
                     /locus_tag="ACHKIR_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HFO9990808.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDVVCQPQ
                     SQVSCQQWLEAHWRTLTPANFTQLQEAQ"
     gene            complement(23400..24602)
                     /gene="fes"
                     /locus_tag="ACHKIR_003110"
     CDS             complement(23400..24602)
                     /gene="fes"
                     /locus_tag="ACHKIR_003110"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0008849 - enterochelin esterase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HFO9990809.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFRWRDPQGSEEYS
                     TIKRVWVYITGVTDHHQNSQPQSMQRIAGTNVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQA
                     PETPAKEIIWKSERLKNSRRVWIFTTGDATAGERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEQLKAGQVSAEG
                     LRIVLEAGIREPMIMQANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            24845..27087
                     /gene="fepA"
                     /locus_tag="ACHKIR_003111"
                     /pseudo
     CDS             24845..27087
                     /gene="fepA"
                     /locus_tag="ACHKIR_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
     gene            27197..28545
                     /locus_tag="ACHKIR_003112"
     CDS             join(27197..27677,27677..28545)
                     /locus_tag="ACHKIR_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558234.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFO9990810.1"
                     /translation="MKHSFEVKLAAVNHYLAGHAGIISTAKLFQLSHTSLSHWINLFL
                     LHGPRALDCRHKRSYSPEDKLCVVLYALGHSESLPRVAARFNIPSHNMVKNWIKGYRK
                     SGNEAFIRRRKEKSMTRSDDTHENEANMTPEEMKNELRYLRAENAYLKAMQEHLLEKK
                     PPGAGEKTKVIRSLKCGHCQSDLLKAAGLARSTLYYQLSLQKAKDKYADVKQLIASIF
                     HEHRGCYGYRRIHCELQKRGLKFSGKTVRKLMQQLGLKSPVRLKKYRSYRGNMGLAAE
                     NILQRQFKAEAPCEKWVTDITEFRAGGQKLYLSPILDLFNGEIVAWETACRPTEELVK
                     RMLNKGLESLAEGEKPLLHNDQGWHYRIKSYQSALADKGLVQSMSRKGNCLDNAVMEN
                     FLGHLKEEMYYRRDYRSVEELENAVNEYITYWNQKRIKLSLGGLSPLEYRTEYQKAG"
     gene            28699..29319
                     /gene="entD"
                     /locus_tag="ACHKIR_003113"
     CDS             28699..29319
                     /gene="entD"
                     /locus_tag="ACHKIR_003113"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009366 - enterobactin synthetase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009239 - enterobactin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HFO9990811.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFEPASFHEQDLLWLPHYAQLQHAGR
                     KRKAEHLAGRIAAVHALREFGFKYAPPVGERRQPLWPAGLYGSISHCQATALAVVSHQ
                     PTGIDIEEIFSAQAAEELKDSIMTPAEYELLVVSNLPFSVVLTLAFSAKESAFKASKL
                     QTDQGFLDYQIVNWNKQRIIICWENEIFAVQWQIRGKQLITLCQHD"
     gene            29663..30598
                     /locus_tag="ACHKIR_003114"
                     /pseudo
     CDS             29663..30598
                     /locus_tag="ACHKIR_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(30746..>30991)
                     /locus_tag="ACHKIR_003115"
     CDS             complement(30746..>30991)
                     /locus_tag="ACHKIR_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein acyltransferase"
                     /protein_id="HFO9990812.1"
                     /translation="EKYVHNGVVITQSDYIRLNNGRGYKTTLVNGSLLAKFSVAPNTK
                     LINVQNYGGTIVFNNDWEIDWDIYNKSKKEHFFVVTL"
BASE COUNT         7612 a         8437 c         7916 g         7026 t
ORIGIN      
        1 aaatttgacc tcaatttact ggtcatattt gaatgtattt atcaacatct cagcattagt
       61 aaagcggcag aatcattata tataacgccc tctgctgtta gtcagtcgtt acaacgttta
      121 cgtgcgcaat ttaatgaccc gttattcatt cgctcaggga aaggtatcgc gccgactaca
      181 acagggttaa acctgcatca ccatcttgag aaaaatctca gaggccttga acaaacgatc
      241 aatatcgtta ataagtctga actaaagaaa aattttatta tctacggtcc tcagcttatt
      301 tcttgtacta ataacaacat gctaatccgc tgtcttagac aagatacttc aatcgatata
      361 gaatgccatg acatcctgct ttcagctgaa aatgccgaag aactgttggt tcaacgcaaa
      421 actgacctgg caattacact gcaaccagta attagtcgct ctgtcatctg catgccatta
      481 cacactattc gcaattccct tatatgtagc aacaaacatc ctcgtattac tgataccagt
      541 aactatgaac aaattatcgc cgaagaattc accctgttga tatctaaatc agcagggatt
      601 gacgaaattc aaatggacat agatgaacgt tttataaacc gtaaagtttc atttcgcagt
      661 tcttctttgt tgactataat taatagcatt tctgttactg acttattagg cattgtgccc
      721 accgaactgt acgatttaca tcgtgatttt ttgaagttaa aagaaataaa actagaacaa
      781 cctcttcctg cggttaaact ttatatttcc tataacaagg cttcgttgaa caaccttgtc
      841 ttctcccgat tcatagatcg tttaaatgat agcttctgat tcattaccac tgacaaataa
      901 tatattacgc atcaattaat tcattgtgtg aatataaaat aatgtgctct atcttgcttc
      961 gtttatccta taattagcgg attcatctca cgagtcaccc cgtaacgttc agagagcgtt
     1021 tttagatgtc tatttataaa atcccgcttc cactgaatat tctggaagct gcaaaagaac
     1081 gtattacctg gacacttaat accctacccc gtatctgtgt ttctttttcg ggtggtaaag
     1141 attccggatt aatgctgcat ctgacagccg aaattgcccg acaaatgggc aaaaaaatct
     1201 gcgttttgtt tatcgactgg gaagcgcagt tctcatgcac tattaactat gttcagtcat
     1261 tacgtgagtt ttacgccgat gtcatcgaag agttttactg ggttgcgctc ccgcttacga
     1321 cgcaaaattc cctttctcaa taccaacccg aatggcagtg ctgggaacct gatgtcgaat
     1381 gggtgcgtca gcccccgcaa gatgcgataa ccgatcctga ctttttctcc ttttaccagc
     1441 ctggcatgac cttcgaacaa tttgtgcgtg agtttgctga atggttttca caaaaacgtc
     1501 cggcggcgat gatgatcggc atccgtgcag atgagtccta caaccgtttt gtcgccatcg
     1561 ccagtttaaa taaacaacgt tttgccgatg atatgccctg gaccaccgcc gcaccaggcg
     1621 gtcatagctg gtacatttac cccatctacg actggaaagt ggctgatatc tggacctggt
     1681 atgctaacca tcaacagctc tgtaacccac tgtataatat aatgtatcag gcaggcgttc
     1741 ctctgcgtca tatgcgaatt tgcgaacctt ttggcccgga gcaacggcaa ggattatggc
     1801 tctatcacgt tatcgaaccg gatcgctggg ctgctatgtg cgcacgagtc agcggggtaa
     1861 aaagtggcgg catttacgcc ggacatgata atcattttta tggtcatcgg aaaatcctca
     1921 agccagaaca tttagactgg caagaatatg cattattgct gctcaatagc atgccggaaa
     1981 aaacagctga gcattaccgc aataaaatcg ccatttattt gcactggtat cagaaaaaag
     2041 gcatcgaagt tccacaaacc cagcaagggg acattggcgc gaaagatatc ccctcctggc
     2101 ggcgaatatg caaagtttta ctcaataacg attactggtg ccgggcatta tcatttagtc
     2161 ctacgaaagc gaagaactat cagcgttata acgaacggat aaaaggaaaa cgtcaggaat
     2221 gggggatact atgcaacaac gattaacgca ggatttaacc caatttctcg ccagcttgcc
     2281 ggaagatgat cgcatcaaag cgattaatga aatccgcatg gcgatccatc aggttagccc
     2341 ctttcgtgaa gagccagtgg attgcgttct gtgggttaaa aacagccaac ttatacctaa
     2401 tgattacaac ccaaacaacg tggcaccacc cgaaaaaaaa ctgctgaaaa aatccattga
     2461 aacagatggt tttacccaac ccattgtggt cacgcatacg gataaaaatg ccctggaaat
     2521 cgtggatggt tttcaccgcc atgaaattgg taaaggctca tcatcgttaa aattacgctt
     2581 aaaaggttat ttgccggtga cctgtctgga aggtacgcgt aatcaacgca tcgccgcgac
     2641 tattcgtcat aaccgcgctc gtggtcgcca ccaaatcact gccatgtccg aaattgttcg
     2701 tgaactcagc cagttgggat gggacgataa taaaattggc aaagagctgg gaatggacag
     2761 cgacgaagtg ctgcgcctga aacaaattaa cggcctgcaa gagttgtttg ccgaccgtca
     2821 atattcccgt gcctggactg tcaaataact acagctggcg caggcgttca gctgctgcca
     2881 gcaacgtcga ttccttcttg gcaaaacaga gacgaatcag tttatggggg aagggatcgg
     2941 cgcaaaatac cgacagcggg atcgccgcta caccgtgctc ccgcgtcagc cactggcaaa
     3001 acttaacgtc atctagcgaa gaaaccgcgc tgtaatccac cagcaaaaag tatgtacctt
     3061 cgcacggtaa aatctccagc cgactctcac ttaaggcatt caccagaata tcgcgtttct
     3121 ggcgataaaa gtccggtaac gcaagataat gctcaggttc tgcacgtagc atatcggcaa
     3181 gcgccaactg tgccggggta ttcaccgaaa aggtcagata ctgatgcact ttgcggatct
     3241 cggcgctgat gggcgcaggc gcaacgcaat aacccacttt ccagccggtc atatgatagg
     3301 tcttgccaaa cgatgacacc gccaccgctc gctcacgcaa ttgcggatgc gccagcacgc
     3361 tggcatggcc ctgttgtgaa aaattgatgt gctcgtagac ttcatcgcta atgacaaaaa
     3421 tctcgtgccc ggcgatcgcc tgccacaaag cggcgaaatc agcctgctgc cagacagttg
     3481 cactggggtt atgcggggtg ttgaggatca ccagtcgggt gcgctcgctt aacaatgcgg
     3541 caaattcctg ccagtccacg cgaaaatgcg gtggttgcag tgcaatacgt ttcactattc
     3601 cccccgaaag cgcgatggcg ggcgcgtaac tgtcatagct gggatcaaag cagatcactt
     3661 catcaccatt gcgcaccaat gcggtaatcg ctgcatataa cgcttccgtc gcccctgccg
     3721 ttacggtgat atcgctatcg gcatccggtt gatagccata cagacgttcc gttttctgag
     3781 caatcgcctc gcgcaaggcc tgcacgccgg tcatgggcgc gtattggttt gccccctggg
     3841 caacgtggta cgccagccgc tcctgtaaat agcgcggacc atcaaaatca ggaaagcctt
     3901 gtgaaaggtt aatcgcctgg tgttgctgcg ccagcgcgct catccgggtg aaaatagtgg
     3961 tgccaagttg tggaagtttg ctttgtggaa tcagagggtt atttgtcatt tttattgtac
     4021 ctgacgaagt tgcgtgttga aggcactata acacgatgct agtatttggc aatcaagacg
     4081 tttagatgtc taaataaaac aataaggaca acacaacatg cctcacaatc ctatccgcgt
     4141 ggtcgtcggc ccggctaact acttttcaca tcccggaagt ttcaatcacc tgcacgattt
     4201 tttcactgat gaacaacttt ctcgcgcggt gtggatctac ggcgaacgcg ccattgctgc
     4261 ggcacaaacc aaactgccgc ctgcgtttga actgccaggg gtaaagcata ttttgtttcg
     4321 tggtcattgc agcgaaagcg atgtgcaaca actggcggcg gagtccggtg acgatcgcag
     4381 cgtggtgatt ggcgtcggcg gcggcgcact actcgacacc gcgaaagccc tcgcccgccg
     4441 tctcggtctg ccgtttgttg ccgttccgac gatcgccgcc acctgcgccg cctggacacc
     4501 gctctccgtc tggtataacg atgccggaca ggcgctgcat tatgagattt tcgacgacgc
     4561 caattttatg gtgctggtgg agccagagat tatcctcaat gcgccgcaag aatatctgct
     4621 ggcggggatc ggtgacacgc tggcgaaatg gtatgaagcg gtggtgctgg ctccgcaacc
     4681 agaaacgttg ccgctaaccg tgcggctggg gatcaataat gcgcaggcca ttcgcgacgt
     4741 cttgttaaac agtagcgaac aggcgctgag cgatcagcaa aaacaacagt taacgcaatc
     4801 attttgcgat gtggtggatg ctattattgc tggtggtggg atggtcggtg gtctgggcga
     4861 tcgttttacg cgtgtggcgg cggcccatgc cgtgcataac ggtctgaccg ttttgccgca
     4921 aaccgagaag tttctccacg gcaccaaagt cgcctacgga attctggtgc aaagcgcctt
     4981 gctgggtcag gatgatgtgc tggcgcaatt aacagaggcg tatcagcgtt ttcatctgcc
     5041 gactacgctg gcggagctgg aagtggatat caataatcag gtggagatcg acaaaatgat
     5101 tgcccacacc ctacgtccgg tggaatccat tcattacctg ccagtcacgc tgacaccaga
     5161 tacgttgcgt gcagcgttcg aaaacgtgga atcgtttaaa gcctgacgta caaatttagc
     5221 agcagcgcgc gccgcctttt ccaccgtagc gcgcgtcctg ccgctcccgg aaaaactcct
     5281 cgtaggtcat cggtgtttga tcgggatggt taacccgcat atgttcgaca tagttgtcgt
     5341 aatcaggcat accaatcatc agtttcgccg cctgacctaa atattttccg gcttttgcca
     5401 gtgaatcaaa catctaaacc tccggtaata caaatgccct cctgaattaa gagggcttaa
     5461 ttgctatcaa cacgctcggg atgagagtaa ggggaacgct cagcacattc cccctcactc
     5521 tgactttagt gcgcgccttt cgcctgcgcc acgatctcct cgacattttc cggcatcggc
     5581 tcatatggcg tttctttcgc cgttggcttc ggctctttca atgccaccag tgccgtctta
     5641 atcgagaaca gcgccagaac caccacaacc accataaaga agatggttaa tccggcatcc
     5701 agacggttgt tgaacaccag ttgcgccagt tgtgactcag tatactgcga cggaatattg
     5761 ccgctgtcga tcattgcctg gaacttgtta gcaatcgcca ggaagccgac tttcgcatcc
     5821 gggctaaacg ctttctgcca gcctgcggtc agggtacaaa tcagcagcca ggccgttggt
     5881 accagcgcca cccaggcgta acgttgacgt ttcattttga acagcaccac agcacagagc
     5941 atcagcgcca tccctgccag catctggttg gcaataccaa acagcggcca caaggtgtta
     6001 ataccgccca gcggatcgac cacgccctga tgcaggaagt agccccacgc cagcacgcac
     6061 agcgccgttg ccagcaggtt agcaggcagt gaatcggtcc gtttcaggcc cggagacacc
     6121 acgcccagca gatcctgcaa cataaagcgc gcagcacgcg tacccgcatc caccgccgtc
     6181 agaataaaca gtgcttcaaa cagaatggcg aagtgatacc agaacgccac atccatcatg
     6241 ccgcccagcg caccgtgcag aatgtaggcc atccccaccg ccagggtcgg cgcaccgccc
     6301 gcacgagaaa tgatcgactg ttcacccact tcgctggcaa tctggtttag cgtatccggg
     6361 gtaatcgcaa agccccagct actcaccacc tgcgcggcag aagcaaccac atccgccgtc
     6421 cctgccggag ccagcaccgc catcgggctg ttcatggcga agtacacacc cggatcgatg
     6481 atacaggcgg aaaccagcgc cataatcgcc acgaaggatt ccatcaacat cccaccgtag
     6541 ccgataaagc acgcctgccc ttcgttcgcc agcatcttcg gtgtagtgcc ggaagagatc
     6601 agtgcatgga agccagacac cgcgccacag gcgatggtga taaacaggaa cgggaacagg
     6661 ttaccggtcc ataccgggcc agtgccatcg acaaatttag ttagcgcagg catggtcagc
     6721 gtcgggcgca taatcaaaat gcccaccgcc agaccgacga tagtcccgat tttcaggaag
     6781 gttgagaggt agtcacgcgg agccagcagc aaccacaccg gcagcaccgc cgccacaaaa
     6841 ccgtaaccca ccagcatcca ggtcagctgc acgccggtaa agtcaaagta cggtgcccac
     6901 gtcgggcttt ctgccaccca gccgccagag ataatggcga aaataaggaa caccagacca
     6961 atcaccgaca cttcgccaat acgccccgga cgcaaatagc gcaggtagat ccccataaac
     7021 agcgccagcg gaatggtgaa cgcaacggta tacgttcccc acgggctatg agtcagggct
     7081 ttcaccacga tcatcgccag cacggcaagg ataatgacca tgatcataaa gcaggccacc
     7141 agcgcaatca ccccggcggt tggccccatc tcttctttga ccaactcacc cagcgagcga
     7201 ccatcgcggc gcgtagaaac aaacagcacc atgaaatcct gcaccgcacc ggcgagaacc
     7261 accccagcga gcagccagat catccctggc aggtaaccca tttgcgccgc cagtaccggc
     7321 ccaaccaacg gacctgctcc ggcaatggcc gcaaaatggt gaccgaacag cacttttttg
     7381 tccgtcggca catagtccag cccgtcgtta tggcgcaccg ctggcgtcat acgcgtcggg
     7441 tcaaccgcca gcacattttt ggcgatatac agcccataaa aacggtaagc gatcagatag
     7501 atacagaccg acgccaccac aatccacagc gcgttgatct gttccccacg atttaaagca
     7561 atgtatccca gagcaaatgc tcccattaca gagagcactg tccagacgag gtatttccct
     7621 gatttgttca tagttgttat ccgtgtgcgt gtccatagag atgttacatt ttgtttctat
     7681 aacatctttg tacaatttaa caacacggca accatgtaaa tcggagttga aaccaatatt
     7741 taacttaaca atgttaactc gatcactccg ttaaccgagt tttcctgcaa tctcatttaa
     7801 ttctgtcggg ctcatcccaa aactgccgta cccagccgac aagtgcagca acgccgcccc
     7861 tgttcatcga aaacaacaat ttcccagctc tgattttgcc gaccaagatg cagcggctgg
     7921 cagacgccgc gtacctttcc ctcagacacc gggcgatgat gtgtggcgtt aagttccgtg
     7981 cccaccacac actgtccgtc gcgagtcatc ataaatccgg ccatcgatcc cagcgtttcc
     8041 gccagcgccg ccgacgcgcc gccatgtagt aaaccgaacg gctgatgagt acgggtatca
     8101 accggcattt cggcttccag cacatcatcg cccaggcggg tatacacaat tcccagatgc
     8161 gccaccattg tgttatcgct ggtggcgttc agctcgtcga gcgttaaatg gcgtttccag
     8221 atcatgctta tgcccccagc gtagagccgc catctaccac aatatcctgt agggtgatat
     8281 ggctggcgag gtcagaggcg aggaacaaaa tcgtgttggc gatctcttgt ggacgggcga
     8341 ttttccccag cggaatgcca agtttaaact gctcgccaaa gccgcgaata cgctgctctt
     8401 cggcgtcatc gctcacccac agcgtgcgtt gcatatcggt gtcggtggag cctggcgaaa
     8461 ccacattaca gcgcacgccg ctacccgcca gttccagccc aacgctcaac gccaggcttt
     8521 tcagcgccgc tttcgacgcg ccataagcac tcataccaat gcgcggcgtg tgtgcggcgt
     8581 cggacgcaac agtaacaatc gccccgcccc gctgacggcg aaactggttc atggtctgct
     8641 ggaacaggtt aaacgcaccg ccgacattaa ccgcaaaagt ctgctgccag tcctccttac
     8701 tgagctgatc ggtcgcgccc atgcgcaaaa ttcccgccgc attgaccagc acgtccagtc
     8761 gctccgtttc agctaacagt cgctgacata cctgcgccac ctgcgcagcg tcggcaacat
     8821 ccatcacttc ggtcgcaaag ggatattgct cctgagtgaa cgcttgatca aaacctgtaa
     8881 ctttcgctcc cgcctcaaca aacgccagcg ccgtggcgta gccgatacct ttccccgcgc
     8941 cagttaccca gacattttta ccgctgaaat ccattatttc acctcgcggg agagtagctt
     9001 ccaccaggcg tcgatggtcg ggtttttcgc cagcatgaca aagtcgatat cgccatgcac
     9061 tttgcgccag cgcgccgcca gcgccatcat ccgcaccgaa tccagaccgt agtcgatcag
     9121 gttgtcgtca tcgaacggtt catcggactc gtccagcaac ggcaggatca cctcacgcag
     9181 cgccgctttg ctggcgggga taggtgctgg cagtaattct tcggtcatca ccactcggcc
     9241 agaacgtccg gccacatatt tcagcgacat caaatgctcg tcacggctga aatcggccag
     9301 cgcatctgcc accataaacg gtttaatatc gcgcataaat gcgtcggttg cggtggtcat
     9361 acagccaatg tgggcatata ccccggtaat aatcagctgg ttacgtccac tctctttcag
     9421 catttgctcc agcggggaac gatgaaacgc gctgtagcgc cacttcacca gcaccgtgtc
     9481 gtcggcatct ggcgtcaggc tatccaccac cttttgctgt tccggcgaac gggtcaggcc
     9541 aggcccccac atatcattca acagcgcccg atcttcatcg ctctgctctt tcggctgggc
     9601 ggtgtaataa accgggatat tgtgctgttt gcagtagtcg cgtagtgccg caatattcgc
     9661 gatcacctgc tccatcatcg ggcagttctc gccccagaag ctgacaaaat agtcctgcat
     9721 atcatggatt aacaacgcgg cacgttgcgg ttcaaaggcc cagtcgactt tattctgcgg
     9781 aatatcgtga gactccggca gtgcgtaagc ctgtaatttt ggaatagcca tcgtgttctc
     9841 tccttcaggc tgatgcgcgt gacgccagcc actgacgtaa ttgtttttta tcgactttcc
     9901 cgaccgccgt cagcggaagt gaatctacac actccacgcg atccggtaat ttaaattcgg
     9961 caataccctg ttcacgcagg aaacgacgca cctgcaccgc gcgcagcggc tcttttacca
    10021 ccagataagc acagcttttt tcgcccatca gctcatcttc catgctcacc agtgcggcgt
    10081 agatcaccgc cggatggcgc agcagcaggt tttcgatctc ttcggcagcg atcttctcgc
    10141 cgccacggtt gatctgatct ttctcgcgcc cctgcacagt gatgtaaccc tctggatcaa
    10201 tagagatcag atcgccggaa cagtaaaaac cgttggcatc aaaggcgctg gcattgtgct
    10261 gcgggctttt gtaatagcca cggaaggtat acggaccgcg ggtcatcagg cgtccaactt
    10321 ccccttgcgg cagtggattt ccttcggcat cggcaaccca cacttcatcg tccggacaca
    10381 ttgggtaacc ctgggtatgg ataattttct ccgcgctatc atcaagtcgg gtgtagttca
    10441 ctagtccttc cgccatgccg aatacctgct gtaactggca gccaatctct gcgggaatac
    10501 gcgccgcaag cgtggcagaa agacgtgcac caccgacctg taacagtttc agcgaggcaa
    10561 gctgcgcccg gctttcgcct tcagccagtg cctgcaacca caggctgact gcgggcggca
    10621 ccagcgcggt aacgttgacc tgatgttttt caatcaatgg gaagcaaagc gtggcgctgg
    10681 ggtcggcagc cagaacgaca gtgccgccag cgagaaatac ccccaacgat cccggcgaac
    10741 tcatggcgta gttatgagcc gccgggatcg cgcacaggta gcgcgtctgt tgtgtgaact
    10801 gacaaatctc gacgctacga cgcacgctgt agtagtagtc gttatgagtg cgcggtatca
    10861 gtttcggagt gccggtggtg ccgccggaaa gctggaaata ggccacttca tcagcaggag
    10921 atggcgtggc agtaaaatcc tcagccggat ggttaatcgc atcctgcaag ttatgctcac
    10981 cgctgtcgtt gagcagctgc accacgcgaa tggaagaatg ctctgcaaca aatgtgttga
    11041 ggaaatcatc cccgctaaat agcgcatgtt gacgatcggc aatcagtaac gccggttcaa
    11101 tctggctggc ataggcgttc agttcactac gctgatggct gaacagcgcc agcaccggcg
    11161 caacgcccag cttcagcagc gcgaaaaagg taatatacaa ttcagcgacg ttacccagtt
    11221 gtaccagcgc ggtttcacca ggtttaatgc cctgacggcg caaactacag gcgaggttat
    11281 ccgccgcctg attcagctcc cggtaactca actgccgctc gccgtcgata accgcgatgc
    11341 tgtcactcgc agcgtggcga gtcagaatgt cggtcagtgg caaatcctgc cagtagcctt
    11401 tttcccgata gcgacgggca aactcttccg gccagcgggt gaatggaatg ctcatcctcg
    11461 ctccttaatg caatccaaaa acgttcaaca tggtagaaag tttgacgcct gtttcgcgcc
    11521 actcacccaa cggtgaggac gcaggcacaa tccccgctcc ggcaaacaga cgcacctgat
    11581 tttcccgcag cttcgcgcag cggatggtca ccacccattc accgttacct tcgctgtcac
    11641 accaacccac aatgccgcca aacagttcgc ggtcgaacgg ctccagttca gcaataacct
    11701 gagtcgcggc ctgatgcggg aaaccgctca gcgcgggggt cggatgcagc agacaggcca
    11761 gagtcagtgc gttttcttgc gaattcgctt taccttcaaa gggagttgcg agatgccaca
    11821 gcgtcggcgt ggtaatcaat tgtggagagg agggaacgtg taactcacta ctgcgtttgc
    11881 gcagtacctc tttcatcgcc tgagtcacca gttcatgttc atggcgatct ttttctgacg
    11941 ccagcagacg attacccgct tcgcgatcga gcacttcatc cggctgacga cgcgcggaac
    12001 cggctaacgg aatggagcta aaacgctcgc cgtctttacg tagcagcagt tccgggctgg
    12061 cccccagcag gacgccgcca tcagccagcg ggacatggaa gttgtaacta accgggtttt
    12121 gcgcaatcaa ccgttccagc aatacgccac tatcaatggc ggcgtcagtg gtgatatcaa
    12181 tcaaccgtga caacaccact ttgtcgacct gcggcgtggc ggtaagtgcg gcggcgcggg
    12241 caaccatctg ttcaaacgtg gtttgttccg gaattgcctg gcgttccacc acattcagcg
    12301 actggctgcg ggtgaaacgg cgtgcggaag cttgtttttc ctgacgggag aacgactgcc
    12361 aggattcggg aatatacagc gatgaaggct gacgtggatc gaagggaatc gccccgacca
    12421 tcaccggatt tttgatgccc tgcgctttgg caccggcaaa cagcgcggcg agtttttgct
    12481 ggaagggact gtcgggcgaa tccccgttca cagccggttc atcgaagcgg gcgaaacatc
    12541 ctgacgtcgt aaaactgcgg tacggcgaca taaagaaaaa gcgattgggc gcaagtgttg
    12601 ccatggtctg ctgtacttcc tcagccagtg acgtatccat atcatcctcc acaaaatgat
    12661 aaaggcttta ataatgatta tcatttatat tttcggtcgc taacctaaaa gcaaacgcgc
    12721 actatgtcaa ctcttgaggt aacgcaatct gcaactcggg gttgcatctg gttgtggtta
    12781 caatgaaaat gagaagcatt aacttcatta ataacaggaa gctgatttgt gagactcgtc
    12841 ccgctctacc gcaacgccct tctattaaca ggacttttgc tttcaggaat agccgtagtt
    12901 caggccgccg actggccgcg tcagattact gacagccggg gcactcatac cctggaaagc
    12961 cagccgcagc gtattgtttc caccagcgtc accctgactg gctcactgct ggcgattgat
    13021 gctccggtga tcgccagcgg cgcgaccacg ccgaataacc gcgtcgcgga tgaccagggc
    13081 tttttacgcc agtggagcaa ggtggcgaaa gaacgcaaac tgcaacggct ctatatcggc
    13141 gagccgagtg cagaaaccgt tgccgcgcaa atgccggatc tgattttaat tagcgcaacc
    13201 ggcggtgatt cagcgctggc gctgtacgat cagctttcca ccatcgcccc aacattaatc
    13261 atcaattacg acgacaaaag ctggcagtcg ctgttaacgc agcttggcga aattaccggg
    13321 catgagaaac aagcggcaga gcggattgca cagtttgata agcaactggc ggcggcgaaa
    13381 gagcaaatca aattaccgcc gcagccggtc actgccattg tctataccgc cgctgcacac
    13441 agtgccaatc tctggacgcc ggaatcagca caagggcaga tgctggaaca actcggcttt
    13501 acgttggcga aattgcccgc agggttaaac gccagccaaa gccagggcaa acgccatgac
    13561 atcattcagc ttggtgggga aaatctggct tcagggttaa atggcgagtc actattcctg
    13621 ttcgccggtg atcagaaaga tgccgatgcg atttatgcta atccgctgct cgcgcacctg
    13681 cctgcagtac aaaacaagca ggtttatgcg ctgggaaccg agacgttccg tctggattac
    13741 tacagcgcca tgcaagtgct ggaacggctt aaggcgctgt tttaagcatt aactgtcgga
    13801 cgctgtcacc tgcggcggcg tctggcgaaa acgccgcaac tccaccagca ccagcaataa
    13861 caacacgccg ataatcaaca aaccaaaacc gctcgcgctt gcggaagcaa ccggtgtcat
    13921 catcgcgccc aaaccgccca gcagcgccgc gcctatggca tcgcccgtca cgttttgcgc
    13981 cgtccacaaa ccgttaatcc gccctaacat cgcttccggg gtttgcgttt gcagcattgt
    14041 gtattgcagc aacgaactaa ccgcgctcag ccagccgaac agcgccagac aaaccacgcc
    14101 taaaatccac atcggcatca ggccaaacag accaatggcg aggaacgatc ccagcgtgga
    14161 aagcagcatc aataaccctg gtcgcgcgct atgtgccagc ttcccgctgg ttaacgcgcc
    14221 aatagccgca ccgagcggga tcgccgcata gagaaaacca atctgtgccg ccgacatctg
    14281 ccagttgtca gccagcgccg gatacaggac ccgcaccgcg ctcgccatcg tcaataaacc
    14341 acccaacagc gcaatccctc ctaccagcgg gctggcgagc agaaaacgaa atcctgccag
    14401 taatgatttc aacggatgct cacgcggctg cggtggtggt ggtaacgccg gaaggcttaa
    14461 caacggtagc aaggtaataa acgtgcccgc cgccgccagc ccgtagttcc aggccacacc
    14521 gccagtcgcc agcagtaaac cgccaatcat gggcgaaatc accgatccca gacgcacggt
    14581 caacatggtg atcgccccgg cctgcattaa gttttcacgc cctaccagtg ctggtgtcgc
    14641 cgccagtagc gccgtaacgc caagtgatgc gaaaaaacca tcccataaac caagtaaata
    14701 gattgccagc aatgacggct ccggcagcag tgcattaagg cacagtccaa tgaagccaat
    14761 gccacaggtg ccgcgcgcca gcaaaatcac ttttttgcgc tcatagcgat ccgccagcac
    14821 accgccgacc atcaggccaa caaacatcgc gccgccggtc agcgtcaccg aaagccccac
    14881 ctgccaggta gaatgcgtca ttatctggat ctgcaccggt accgcgacgc cgagcaaacc
    14941 cagagacaca attgagataa aacgagcgag gaatactgcg cgaaacgccg ggtgcgtttt
    15001 caacaggctg aggttaagca gccaggattg tttattcatt acaatgcctt gccatcaaat
    15061 attttaaata cctattctta ggctgcacat gctaacatat ccaaataaga tcgataacga
    15121 taattaattt cattatcatg gaagttcgta tgtctggttc tgttgccgtg acacgcgcca
    15181 ttgccgtgcc cggattgctg ttattactga ttatcgcgac ggcattaagc ctgctcattg
    15241 gggcaaaatc actccccgct tccgtagtgc tggaggccct ctccggcacc tgccagagcg
    15301 ccgactgcac catcgtgctc gacgcgcggc tgccgcgcac ccttgccggt ttactggcag
    15361 gcggcgcgct tgggcttgcc ggggcgttaa tgcaaaccct cacccgaaac ccacttgccg
    15421 accccggctt gcttggcgta aacgccggag ccagctttgc cattgtgctg ggtgcggcgc
    15481 tgtttggtta ctcatccgcg caggaacaac tggcgatggc cttcgccggg gcgctggtgg
    15541 cctcgttgat tgttgccttt accggcagcc agggcggcgg gcagttaagt ccggtgcgtt
    15601 taaccctggc gggcgtggcg ctggcggcgg tgctggaagg gctgaccagc ggcatcgccc
    15661 tgcttaatcc tgacgtctac gatcaactgc gtttctggca agccggttcg ctggatattc
    15721 gcaatctata taccttaaaa gttgtgctga tcccggtgct gatcgccgga gcaaccgcgc
    15781 tattactgag ccgcgcgctg aacagtttaa gcctcggcag tgacaccgcg acagcgctgg
    15841 gcagccgcgt ggcgcgcaca cagttgattg gtctgctggc gattaccgtg ctttgtggta
    15901 gtgcgacggc ggtagttggc ccgattgcct ttattggcct gatgatgccg catatggcgc
    15961 gttggttggt tggagcagat catcgctggt cgctgcccgt cacgctactt gccacccctg
    16021 ccctgctgct gtttgccgat atcatcgggc gggtgattgt gcccggcgaa ctgcgagttt
    16081 ccgtggtcag tgcgtttatt ggtgcaccgg tgctgatctt cctcgtgcga cgtaaaacgc
    16141 gaggtggcgc atgatttacg tctctcgccg attaattatc acctgtttgc tgctggtttc
    16201 cgcctgtgtg gttgcaggta tctggggatt acgcagcggt gccatcacgc tggaaacctc
    16261 gcaggtattc gccgcgctga tgggcgatgc gccgcgcagt atgacgatgg tggtcaccga
    16321 atggcgttta ccacgcgtgc tgatggcgct gttgattggc gcggcgctgg gcgtcagcgg
    16381 cgcgattttt cagtcgctga tgcgtaaccc gctcggcagc cctgacgtaa tgggctttaa
    16441 caccggggca tggagcggcg tactggtggc gatggtgctg tttggtcagg atctgacggc
    16501 tatcgcgctg gcagcaatgg cggggggtat tgtcacctcg ctgttggtct ggctgctcgc
    16561 ctggcgtaac ggtatcgaca cctttcggtt gattattatc ggtatcggtg ttcgcgccat
    16621 gctggtggcc tttaatacct ggctgttgct gaaagcgtct ttagaaacgg cgctaacagc
    16681 gggtttgtgg aatgccggat cgctcaacgg cctgacgtgg gcaaaaacct caccttccgc
    16741 gccaatcatt atattgatgc tcattgccgc ctccttgctg gtgcggcgaa tgcgcttgct
    16801 ggaaatgggc gatgataccg catgtgcgct gggcgtcagc gtcgaacgtt cgcggctgtt
    16861 aatgatgctg gtcgccgtag tgcttaccgc tgcggcaaca gcgcttgccg ggccgatttc
    16921 ctttattgct ttagtcgcgc cgcacattgc ccgccgcatt agtggcaccg ctcgctgggg
    16981 gctaacccag gcggcgctgt gcggggcgtt gttactgctg gtggccgatc tctgcgccca
    17041 actactgttt atgccgtatc aacttccggt tggcgtcgtt accgtcagcc tcggtggtat
    17101 ttaccttatc gtcttgttaa ttcaggagtc tcgcaaaaaa tgaccgaatc agtagcccgt
    17161 ttgcgcggcg aacagttaac cctcggatat ggcaaatata ccgttgcgga aaatctgact
    17221 gtagaaatac ctgatggtca cttcacggca attatcgggc caaatggctg cggtaaatcc
    17281 acgttgctgc gtaccttaag ccgcctgatg acgcctgctc acgggcatgt ctggctggat
    17341 ggcgagcaca ttcaacatta cgccagtaaa gaggttgcac gccggattgg cctgttggcg
    17401 caaaacgcca ccacgccggg agatatcacc gtgcaggagc tggtggcgcg tgggcgttac
    17461 ccgcatcaac cgctgtttac ccgctggcgc aaagaagatg aagacgcggt aacgaaagcg
    17521 atgcaggcca cgggaatcac tcatctggca gatcaaagcg tggataccct ttctggcgga
    17581 caacgccagc gagcgtggat agcaatggtg ctggcccagg aaacggcaat tatgctgctc
    17641 gatgaaccca cgacctggct ggatatcagt catcagattg atttgctgga actgttaagc
    17701 gaactgaacc gcgagaaagg ctataccctg gcggcggtgc tgcacgatct taatcaggcc
    17761 tgtcgttacg ccagccattt gattgcattg cgggaaggga aaattgttgc tcagggagcg
    17821 ccgaaggaga ttgtcactgc tgaactgatc gagcgcattt atggtctgcg ctgcatgatt
    17881 attgacgatc cggttgccgg aacgccgctc gtggtgccgc tcggacgaac gacaccttca
    17941 accgcgaaga tttaaactaa gtggtctgcc attatggcat cctgttttct ggatgccatc
    18001 gcatggcgca atagcacgcc accacaagcc actataccgc cgatcaacgc agaaagaatc
    18061 acaataatcg ccttacccgg accgtctttt tttacgggta atgacggacg caactgatat
    18121 ttaaacggtg taaaattcac atcgttgaca tttgtttttg ttaattgctc gactagatac
    18181 tgacgattac gtaattcacc gttcaattcc gcaacgtcag tgactgcttt ttcaatttcc
    18241 agtttgcgtt caataccgtc tgcgccgaga gaaatagaga aatcggggtc atctttaacg
    18301 gcctgaccat tactgtatac gggcttttta attcctgccg cgttggcaat gtcgagtgaa
    18361 taattgaggc gctgaatgtt tgcatcaagt tgatttttcg ttttaatgcg atcctgagcc
    18421 agtttttctt tttcaaactg ggttttgatc tccagtttat tacggacgtt ttctatcgac
    18481 tctttcacca ccaacgcaga gatataatcg atatacccgg ccaacacttt ctgtgcctct
    18541 tcactggtgg gtgcggtgaa acttagcgtc caggaagtat acaatgcaga ctcatctttt
    18601 ttcttactgg cattgtcatc aaccgctttc attttttcgc tcaatgcgac aattgcgcga
    18661 tgcaaatcca gttcgtcgat tttcgcctct tttaattggt ccatcacata aggtgttgag
    18721 cgcaggtact cttccagcaa gctaaccgac tgaaacttct tgataaacag gttaaatgct
    18781 tctgtacgat caattttgat atccagatcc agcacacgaa gcttagtgaa tgttttctca
    18841 agcgcctgcc actgaacagg ttctggaggc gtgacaaccg ccgcgctggt ccatttttgc
    18901 ggcaggatga aagagatcag taaacctgcg caggcaaacg caaaaacgac cgccatgacc
    18961 gtttttttgg cccgccataa aacttcaatt agattaagta aatcaacttc attattactg
    19021 ggcgacgcca gaggatactc gggaaaatga gcttcacttc cctgtttaat attcagtgat
    19081 gacatggggt ataacctgaa aatgagtcca ataagagtaa atctccacgg cccccactct
    19141 accagttggt gagatttatc cgaagctgaa gtttgtaaat tactcccact gaatgcaaca
    19201 ttcactctat ttcagagaaa tacccaccaa atttataaat taaataaatg ataaacccat
    19261 aattatttcc tgtaattatg ggtttataga taatattaat ttacctgttt agcgtagcgc
    19321 gaataatcgg cccaattttt tcaaacgccc ctggagagat aatatccaca tgcgcacaat
    19381 cctgacgata gatatccagc tcggctatcc acggcgacca ggcgcgttcg ggattcatac
    19441 cttcctgaag cgcgcgttca gcaacaaaca gcgtcgcttt accgtcaaac ggtacactat
    19501 gagcagtcgt cagcaggcgc acagcatcag cgtagttgcc ttcaatggtg gtaaacaact
    19561 ccgttgaagt acttccctgc tgtgccgcca ggaaggcctc gcgctcgcgg ttaatctccg
    19621 ccagcacttc cgggtccaga ccattggctt ctttttcctg ccagttttgc gtttctggcg
    19681 gccaggtatc cagcaagcca agaaatgcca cctgttcgcc acgggcacgc agtcgcgccg
    19741 caatgccctg cgccagcgta ccgccaagtg aataacccag caggtaataa ggaccgtgcg
    19801 gttgttgttc aagtaacgtt gccagatgcg cttcgcagac ttcatccagg tttgccgccg
    19861 tctgcatggg gccatgaggg cgcggcgact gaatgccgat aatcgaccat tgtgaatcga
    19921 gataacgcga gagcacgcta aactgccagg caaaaccgga ggccggatgg aaacaaaaca
    19981 gcgtcgggcc attaccttca cgcaacggca gaatggtttc gaatcccatg cgctgggtgc
    20041 tgtcttcttc accatcaata atcgttgcca gtttggcgac agttgacgcg accatcacct
    20101 gccccggcgt cacctgacga acaaactgtc gacttaactg cgctgccagt ttcattgcca
    20161 gtagcgaatg accgccaagc gcaaagaaat cagcatcggc atcctgcacg tcacaaccca
    20221 gcaacgacga gaatgccgcg gcgataatcg tttcactgcc cgctttcggc gcacgccccg
    20281 gcgcttgtgc tttcagttcc ggcaacggta aggctttgcg atccagcttg ccgttggcgc
    20341 taagaggtaa ctgtggaagt tgcagcagaa ccacgggcac catatgtggt ggcaatgttt
    20401 cgcgaagctg agcctgtaat gcgctggtat ccaacggcag gcccgattgc gacaccagat
    20461 agcccaccaa ctgacgcgca tcaccaccag tggctgccgc ctggttaatc acacaggcgt
    20521 gggtaacggc ttgttcgaca tccggcagcg cctgcatcac gcgatcgatt tcgcccagtt
    20581 cgatacgctg cccgcgaatt tttagctgat cgtcactgcg cccgaggtac tccaccgcgc
    20641 cgttatccag ccagcgggca acgtctccgg tacggtacat ccgttcgcca ggggcaaaag
    20701 gatcggcaat aaagcggctg gcggtcagat cgggtcgtcc aagatacccc tgcgccagtt
    20761 gaataccggt gagatagaga tctcccgcca cacccggcgg caccggatgc atcatcgcat
    20821 caagaatacg caggcccgta ttccacaccg gataaccaat cggcacactg ctgccgcgca
    20881 cctgtgccag ttcctcgcca aaagccggat accagctgac atctaccgcc gcttccgtcg
    20941 ggccatatag attatgcaac ggcacgctgg ttaactgttg ccattcgcgg cataaatcag
    21001 cgggtaaggc ctcaccacta cagaaaacct gtttcaacgt cgcgcaattc tggcgagcgg
    21061 tttgcggagt cagcgatgca acaaatgccg ccagcatcga cggcacaaag tgcgtggtcg
    21121 ttacaccata ttcggcaaag aattgctgca tagcgagcgg gtcgcggtgc gcttccggtt
    21181 cagccatcac cagtttagcc ccggcaataa acggccagaa aaactcccac accgagacat
    21241 caaaactgca cggcgttttt tgggcaacga catcttcgcc tgtaagcggg tagtggtttt
    21301 gcatccaaag cagacggtta acgatagccg tctgcccgac cattacccct ttcggcctgc
    21361 cggtggagcc agaggtaaag atgatataag ccgtgtggtg cggttgtgaa agtttcagcg
    21421 gcgcactgcc ctgcggtgta agcggggcgt tatagcaaag gctggttaaa tctggaacat
    21481 cggcaaagcg cggcagttga tcgtcggtgg tgattaacag cgacggacgc gcatcttcca
    21541 gcatcatttt caggcgatcg tccggatagc cggtatccag cggtaaccag gccgcaccag
    21601 cctcaacaat tgcatgtagc gccagagtca aaaagaccga gcgcggtagc gccaccgcca
    21661 cgctgtcgcc tggtttaacg ccgcgctcac gcagcagatt cgccagcgcc accacctgct
    21721 cgcgcatttc ccgatagctg aacaggtaac gcgcatctgc cagcgccgga gcatgcggtg
    21781 tttttgccgc ttgttctgcc accagcgcgc tcagcgtggt ttctggaatc tcaacctgag
    21841 tggcgttgat ctgcgccagc tgcgcatatt cacctggcag cataatatcg acatcgccgc
    21901 acaacagcgc aggatccgcg gcgaactggg caatcagcat tttcaggcgt tcagcatgct
    21961 ggattaacgt tggctcagcg taacgctgtt tattggcgag gatttcaata ctcaaatctc
    22021 cgtgttcatc cgggaacagg gccagttcaa ggtcattaac cggaccggtt gccagggtat
    22081 gggtttgcgc ctgaacacca ggaatatcca gttggtaatc aaataccttg atattgagta
    22141 ccggaccgaa cagcggttcc tctcctgccg ctcgcccact gtcacggaca atttgttcgg
    22201 cgtcgtagcg ttgatggcgg cgcatttttt tcagttgtgc tgccagtcgg gttgccagtt
    22261 gcggcagcgt ttcttgcgcc gcaatgtgaa tacccaacgg taaaacgttg agcacgggtc
    22321 cggtagccgt cagcgccgcc gagcccagtc gacgcataaa gataaatccg gcagcgtagt
    22381 ccatgcggtt gcacaatcgc cccagccaca aagccgtcag cgcaagggct aaatcggtac
    22441 gctgcacacc tgaaagttgc gtagccagct ggcggaattc cccgtcggta aattccagtt
    22501 tcaggcgcaa aatatctgcc gaagcgctgc gccccggtaa aggtgccgga gaaagtgacg
    22561 cgggcggtgg cagttgacga cgctgttccg cccagaatgc cgcatcacgc tgccaggctt
    22621 cgctttcgcg gtattgctgg tactcttcca ctacatcagc gaaaggcgta aacggcgaag
    22681 caggcgttgg ttcgccacgc agccatgcgc agtaaatatt ggcgatctgg cgggtaatgg
    22741 ccgggaaact gaagccatcg accagcaaat ggtgataacg ctgataccag taccagcggt
    22801 tatccgccac ctgtatcaac tgatgaaaga ccagtggttt accgctatcg acgcgcagat
    22861 cttgttgcaa atccgcctgc attaatgact gcgcagtacc gtgcggatca atgttggttc
    22921 gcaggtcgat agtttctggc agttcgaacg tcagcgcatc atcgacccac tgccagactt
    22981 cgccgttatc ttccgtaaaa cgcatccgca gcgtatctgc ttgcgctaat ccggcaacca
    23041 ccgcgcgggc cagtaatggc gcatcaacct ctccggttaa ctcaacgtaa tgcgccacgc
    23101 tccaggcgga gggtaaatcc gacagttttt ctgccatcca gatgccgggc tgtgcggcga
    23161 ccaaaggtaa atgctggctc attgtgcctc ctgcaactgg gtgaaattcg ccggtgtcag
    23221 agtacgccag tgggcttcca gccactgctg gcaggacacc tgtgactgcg gctgacacac
    23281 aacgtcccag cctgccggta aggcgcattg ttgcggccac agactgaatt gcccctgcgc
    23341 attgcgcaaa atgtaaaacg ctccctgcgg atcatcgaag ggattactga atgccatatt
    23401 caactcctgt catggaaaag tggttgccag aggtcgatta gcccctgcat caagccaccg
    23461 cgccaacaaa gcgcatcatg tccgccgtca acctgacgcc agaaaatgga ttcttttatg
    23521 gggtgtaatt gcgcatacag cgcctgattg gcctgcatga tcatcggctc gcgaataccc
    23581 gcttccagca caatgcgcag accttcggca ctaacttgac cagctttaag ttgctcaagt
    23641 aaaacacctt cttgctgccc gccccgatgc ggccaccagt acgatcctga ctggcttaat
    23701 acacagccaa agcgttcagg ccagtgcagt ccggcataca gcgcggaaag cccaccaaaa
    23761 ctctgcccgg cgaccacggt gcgatcggcg cgatcgctaa aaggggcaat agctttcacc
    23821 aggggtaata actcttgctg tactgccagc cagaaatccg cattacacgg cagttcgtgg
    23881 gcgcggtgcg tggtgtcaat agcgtcgatc aacacataca cggctggagg aagttgctga
    23941 cgatgggtca acgaagtcag cacaggccag acaggcatac tctgcgccca aaattcgcca
    24001 tcgagcaaaa ctgccagcgg acgttctcct gctgttgcat cgccagtggt aaaaatccat
    24061 acacggcgtg aatttttcaa ccgctcactt ttccagataa tttctttggc aggtgtttct
    24121 ggcgcttgcg gacaatccca tcccggttgt aggggcgctt gcggcatttc gagtgcagaa
    24181 acagcgtgcc ctcgcccgcc tttccagctt tgtggattca gcggatcggc tatcgcctgg
    24241 ggtaatagtt ttcgccagcc ttcacgtaat tcgaggcgat cgggggatgg tgcagaaaaa
    24301 atgtcatcgc gttcggtggg aataaaacag tagctgccgc gccagttggc attaagttgt
    24361 gtcgtccact gccagacgtt ggtgcctgca attcgctgca tcgactgggg ctggctgttc
    24421 tgatggtgat cggtcacacc agtgatgtag acccatacgc gctttatcgt cgagtattct
    24481 tcagaaccct ggggatcacg ccaccggaaa gtgacctcaa acatttcgtc attcagacgc
    24541 tgccattccg ggccatgttt cgactgccac cagctctcac ttcctacttt taacgccgtc
    24601 acaccataac cccatgttta ctgtgcaatt tttcattgat tgcagaaata tattgataat
    24661 attattgata actatttgca tttgcaatag cgtaatggcg cgccgtggga agcgcggaca
    24721 ttaattaacc aactgcactg cgtgtctttc aggatcaaag gttttcgcgg tagcgggatg
    24781 cgtcgtgttg atgacgacca tgcccgacag ttgcaattcg tggcaaaaat gcaggaataa
    24841 aacaatgaac aagaagattc attccctggc cttgttggtc aatctgggga tttatggggt
    24901 agcgcaggca caagagccga ccgatactcc tgtttcacat gacgatacca ttgtcgttac
    24961 cgccgccgag cagaacttgc aggcacctgg cgtttcgacc atcaccgcag atgaaatccg
    25021 caaaaacccg gttgcccgcg atgtgtcgga gatcatccgt accatgccag gcgttaacct
    25081 gaccggtaac tccaccagtg gtcagcgtgg gaataaccgc cagattgata ttcgcggtat
    25141 gggtccggaa aacacgctga ttttgattga cggcaagccg gtaagcagcc gtaactcggt
    25201 gcgtcagggc tggcgtggcg agcgcgatac ccgtggtgat acctctcctg ggtgccacct
    25261 gaaatgattg aacgtattga agttctgcgt ggtccggcag ctgcgcgtta tggcaacggc
    25321 gcggcgggcg gcgtggttaa catcattacc aaaaaaggca gtggtgagtg gcacggctcg
    25381 tgggacgctt atttcaatgc gccagaacat aaagaggaag gtgccaccaa acgcactaac
    25441 tttagcctca ccggtccgct gggcgacgaa ttcagcttcc gcttgtatgg caacctcgac
    25501 aaaactcagg ctgacgcgtg ggatatcaac cagggccatc agtccgcgcg tgccggaacg
    25561 tatgccacga cgttaccagc cgggcgcgaa ggggtgatca acaaagatat taacggcgtg
    25621 gtgcgctggg acttcgcacc attgcaatcg ctggaactgg aagcgggtta cagccgccag
    25681 ggtaacctgt atgcgggcga tacccagaac accaactctg actcctatac ccgctcgaaa
    25741 tatggcgatg aaaccaaccg tctgtatcgc cagaactact cgctgacatg gaacggcggc
    25801 tgggataacg gcgtgaccac cagcaactgg gtgcagtacg aacacacccg taactcgcgt
    25861 attccggaag gtctggcggg cggtaccgaa gggaaattta acgaaaaagc gacacaggat
    25921 ttcgtcgata tcgatctcga tgacgtgatg ctgcacagcg aagttaacct gccgattgat
    25981 ttcctcgtta accagacgct gacgctgggt acggagtgga atcagcagcg gatgaaggac
    26041 ttgagttcca acacccaagc gctcaccggg acgaataccg gcggcgctat tgatggcgtg
    26101 agtgccaccg accgtagccc gtattcaaaa gcagaaattt tctcgctgtt tgccgaaaac
    26161 aatatggagc taactgacag caccatcgta acgccggggc tgcgtttcga tcatcacagt
    26221 attgtcggca ataactggag tccggcgctg aacatatcgc aaggtttagg cgatgacttc
    26281 acgctgaaaa tgggcattgc tcgcgcttat aaagcgccga gtctgtacca gactaaccca
    26341 aactacattc tctacagtaa aggtcagggc tgctatgcca gcgcgggcgg ctgctatctg
    26401 caaggtaatg atgacctgaa agcggaaacc agcatcaaca aagagattgg tctggagttc
    26461 aaacgcgacg gttggctggc gggcgtcacc tggttccgta acgattatcg caataagatt
    26521 gaagcgggct atgtggctgt agggcaaaac gcagtcggca ccgatctcta tcagtgggat
    26581 aacgtaccga aagcggtggt tgaaggtctg gaaggatcgt taaacgtacc ggttagcgaa
    26641 acggtgatgt ggaccaataa catcacttat atgctgaaga gtgaaaacaa aaccacgggc
    26701 gaccgtttgt cgatcatccc ggagtatacg ttgaactcaa cgctgagctg gcaggcacgg
    26761 gaagatttgt cgatgcaaac aaccttcacc tggtacggca agcagcagcc gaagaagtac
    26821 aactataaag gtcagccagc ggttggaccg gaaaccaaag aaatcagtcc ttacagcatt
    26881 gtgggtctga gcgcgacctg ggatgtgacg aagaatgtca gtctgaccgg cggcgtggac
    26941 aacctgttcg acaaacgttt gtggcgtgcg ggtaatgccc agaccacggg cgatctggca
    27001 ggagccaact atatcgccgg tgccggggcg tatacctata acgagccggg acgtacgtgg
    27061 tatatgagca ttaatactca cttctgatgc taacgtcaga ttgttgacaa agtgcgcgta
    27121 attcatgccg gatgcggcgt gaaactgaac tgcaccccaa atgttggaca taatctgaca
    27181 tctgaaggtg cagtttatga aacattcatt tgaagtaaaa cttgccgctg taaatcatta
    27241 cctggctggt catgcaggca tcatttctac ggcaaaactc tttcaacttt ctcacaccag
    27301 cctctcacac tggattaatc tttttcttct tcacggtcct cgggcactgg attgcagaca
    27361 caagcgtagc tattctcctg aagataaact ttgcgtggtt ctttatgctc tcgggcattc
    27421 tgagtctcta ccccgggtag ctgcccggtt taacattccc agccacaaca tggttaaaaa
    27481 ctggataaaa ggctaccgca aatctggtaa tgaggccttt atcaggcgca ggaaggagaa
    27541 aagcatgacc cgttctgatg atacccatga aaacgaggca aacatgacgc cggaagagat
    27601 gaagaatgag ctgcgttatc ttcgcgcaga aaatgcatat ctgaaagcca tgcaggagca
    27661 tctcctggaa aaaaagcgcc aggagctgga gaaaaaacga aagtcatccg gagcctgaag
    27721 tgcggacact gccagtctga cctgttaaag gctgcagggc tggccagaag cacgctgtac
    27781 taccagctgt cactgcagaa ggctaaggac aagtatgctg atgtgaaaca gctcatagcc
    27841 tccatcttcc atgaacaccg gggatgttat gggtaccggc ggatccattg tgagcttcag
    27901 aaaagaggtc ttaagttcag tggcaaaacg gtgcgaaaac tgatgcaaca gctcggcctg
    27961 aagtctcctg tgcgcctgaa gaaataccgg tcttaccggg gaaatatggg actcgctgca
    28021 gaaaatatcc ttcagcggca gttcaaagcg gaagctccct gtgagaaatg ggtgacagat
    28081 atcaccgaat tcagggctgg cggacaaaaa ctgtacctgt ccccgatact tgatttgttt
    28141 aacggggaaa tcgtggcgtg ggaaactgcc tgcaggccta cagaggagct ggtgaagcgc
    28201 atgttaaata aggggctgga aagccttgca gaaggagaaa aaccgctgct ccacaatgat
    28261 caagggtggc attaccggat aaaaagttat cagtctgccc tggcagacaa agggttagtg
    28321 cagagcatgt cacgcaaggg caactgtctt gataatgcgg taatggagaa tttcttaggt
    28381 cacctgaaag aagaaatgta ctatcgccgg gactacagaa gtgtagaaga gctggaaaat
    28441 gccgttaacg aatacataac ttactggaat caaaaaagaa taaaactcag tcttggggga
    28501 ctcagcccgt tagaataccg gactgagtat caaaaagccg gttaactgaa actgtccagg
    28561 ttttggggtt cagttcagaa cgccttatcc ggcctacaaa aatcttgcca attcaatata
    28621 ttgcaggata gtgtaggcct gataagcgta gcgcatcagg cagttttgcg tttgtcatca
    28681 gtctctaata tggtcgatat gaaaactacg catacctccc tcccctttgc cggacatacg
    28741 ctgcattttg ttgagttcga gccagcaagt tttcacgaac aggatttact ctggctgccg
    28801 cattatgcgc aactgcaaca tgccggacgg aaacgcaaag ccgaacattt agccggacgg
    28861 attgctgctg ttcatgcttt acgggaattt ggctttaagt acgccccccc tgtcggtgaa
    28921 cgtcgccagc cactttggcc tgccgggtta tacggcagta ttagtcactg ccaggctacg
    28981 gcattagcgg tggtatccca tcaaccaacg ggtattgata tcgaagagat cttctctgca
    29041 caagcagcgg aagagttgaa agacagcatt atgacaccag cagaatacga gctgcttgtg
    29101 gtcagcaatt tacccttttc tgtggtgctt acactggcgt tttcagcgaa agagagtgcg
    29161 tttaaggcaa gcaagttaca gacagatcaa ggttttctgg attatcagat agtgaactgg
    29221 aataaacagc ggatcattat ttgttgggag aacgagatat ttgctgtgca atggcagata
    29281 agaggaaaac aactcataac gctttgtcaa cacgattaat ggataaccct tactgtggtt
    29341 agccagcaac cattgcttgt agtagtcaag taatattgac caccgtttta cgctaagaac
    29401 gttatctgtt ctctaatttt ttcggtgtca gcccaccgtt ataatggtga gatctgacgc
    29461 tattatagtt gttcaatata caaccactga tttgctgcca tatatccggc gtcgttagtg
    29521 gactgatgac ccgcgccatg atggcatccg caccctgttt cctaacctat gttaccaccc
    29581 cgacaccgtt caggttgtta cagagtcgat tatgcgatct gatcaatcgc tgaatatccc
    29641 aaatcaccaa ccggacggag cagtgccgat catagcacca atacccagaa ctgaacgacg
    29701 cctgatccag aaaacgatcc ataaaacaaa ggacaaaaaa ccacgcccgg cggctcacag
    29761 ccatgctgat gctgcatcgt ggtgacaccg tcagtcatgc tgccagaaca ctctgctgtg
    29821 ctcgctcatc gattggtcgc tggattaact ggttcacgct gtcgggtgtt gaaggcctga
    29881 agtcattgcc gtcaggacgt gagaggcgct ggccgttcga gcatatttgc gcattgctgc
    29941 gtgagcttgt taagcattct cccggcgatg gttacctgca gccgaactgg tatggcgcag
    30001 agcagcacca acactgcgta tccggggccc gaataaggaa gaaaaaatgg cagcgattaa
    30061 tgtagcgtta gtcagttgta gcatagaaca ccctgtgttt tacgaagatg aagtggagat
    30121 ccacctcaat cccaaaatcg gcgcagactg gcagttacgc gggcagcaaa agcgcgttgt
    30181 tacgccgggc cggaatgaaa agtactatct tgccggtgcg ctgcacagtg gtacgggcaa
    30241 agtcagctac gtgggtggaa acagtaaagg ttcatcctta tttatcaggc tgttaaaaca
    30301 tctgaaggag acatactgac gggcaaaaaa gattacgctc attgtcgata actacatcac
    30361 ccacaaaagc tgtgaaacgt tgcgctggct gaaagcaaac ccaaagttca gggtgattta
    30421 tcagccaatt tactcgccat gggtcaatca tgtcgaacgg ttgtggcagg cgcttcatga
    30481 aacaataacc cgaaatcacc agtgccgttc aatgtggcaa ctgttgaaaa aggttcgtca
    30541 ctttatggat accgtcagcc cattccccgg tggaaagcat gggctggcta aagtgtagcg
    30601 gtattaggat cagctactta gaagcgaatg gagtaactgg catagcacag gggaatttaa
    30661 ctactcagca tatatatgca agacttgaat aatatgtggg cgcgtaaata gctcataaaa
    30721 atttcaacaa cactccctcg ctgtattata aagtaaccac aaaaaaatgt tcttttttgg
    30781 atttgttata aatatcccag tcaatttccc aatcattatt aaaaacaata gttccaccat
    30841 aattctggac atttattaac ttggtgtttg gtgcaacgga aaactttgca agtaagctac
    30901 cgttaaccaa tgtagtctta tatcccctac cgttattaag tcttatgtaa tcagattgtg
    30961 ttattacgac cccgttgtgt acatattttt c
//
LOCUS       DAZGQL010000063        30802 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.063, whole genome shotgun sequence.
ACCESSION   DAZGQL010000063 DAZGQL010000000
VERSION     DAZGQL010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30802
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.063"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(729..1094)
                     /gene="cdiI"
                     /locus_tag="ACHKIR_003116"
     CDS             complement(729..1094)
                     /gene="cdiI"
                     /locus_tag="ACHKIR_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015704291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease toxin immunity protein CdiI"
                     /protein_id="HFO9990813.1"
                     /translation="MNKYLFELPYERSEPGWTIRSYFDLMYNENRFLDAVENIVNKES
                     YILDGIYCNFPDMNSYDESEHFEGVEFAVGYPPDEDDIVIVSEETCFEYVRLACEKYL
                     QLHPEDTEKVNKLLSKIPS"
     gene            complement(1103..10867)
                     /gene="cdiA"
                     /locus_tag="ACHKIR_003117"
     CDS             complement(1103..10867)
                     /gene="cdiA"
                     /locus_tag="ACHKIR_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001385946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contact-dependent inhibition toxin CdiA"
                     /protein_id="HFO9990814.1"
                     /translation="MHQPPVRFTYRLLSYLISTIIAGQPLLPAVGAVITPQNGAGMDK
                     AANGVPVVNIATPNGAGISHNRFTDYNVGKEGLILNNATGKLNPTQLGGLIQNNPNLK
                     AGGEAKGIINEVTGGNRSLLQGYTEVAGKAANVMVANPYGITCDGCGFINTPHATLTT
                     GKPVMNADGSLQALEVTEGSITINGAGLDGTWSDAVSIIARATEVNAALHAKDLTVTA
                     GANRITADGRVSALKGEGNVPKVAVDTGALGGMYARRIHLTSTESGVGVNLGNLYARE
                     GDIILSSSGKLVLKNSLAGGSTTVTGTDVSLSGDNKAGGNLSVTGTTGLTLNQSRLVT
                     DKNLVLSSSGQIVQNGGELTAGQNAMLSALHLNQTSGAVNAAENVTLTTTDDTTLKGR
                     SVAGKTLTVSSGSLNNGGTLVAGRDATVKTGTFSNTGAVQGNGLKVTATDLTSTGSIK
                     SGSTLDISARNATLSGDAGAKDSARVTVSGTLENRGRLISDDVLTLSATQINNSGTLS
                     GAKELVASADTLTTTEKSVTNSDGNLMLNSASSTLAGETSAGGTVSVKGNSLKTTTTA
                     QTQGNSVSVDVQNAQLDGTQAARDILTLNASEKLTHSGKSSAPSLSLSAPELTSSGVL
                     VASALNTQSQTLTNSGLLQGEASLTVNTQRLDNQQNGTLYSAADLTLDIPDIRNSGLI
                     TGDNGLTLNTASLSNPGKITADTLNVRATTLDGDGLLQGAAALALAGDTLSQGRHGRW
                     LTAGDLSLRGKTLNTAGTTQGQNLTVQADRWANRGSVLATGNLTASVTGQLTSTGDIM
                     SQGDTTLNAATTDNRGSLLSAGTLSLDGNSLDNSGTVQGNHVTLHHRSTDNSGTVTGL
                     SGLTLHSADGLTNSGALLSQNSLVLSAGDVTNSGRIQGQNITLDASSLTSSGAVQSAL
                     DLALTLSGDVIAATGSKITALGDARLSGKVLGNQGLISAKTLEVNGDSLSNSGEISGV
                     NSLNVTLSGNLQQHGKMLTGGALNVNARDISNSGQLQGADNRITASSLANSGRVQGES
                     GLTLTLLNALTNQTSGVLLSQNVSALSAPALTNDGTIQGNGKTTLSAATQAHNSGKIL
                     SGGELTFTTPDYSGSGWLQATDLLLNVAKLAGNGTVMAANQATLTGNSLTNRGLFQAA
                     QLNVNTQTITNSGTLLGNQGLTIKGNNLNNAGGKVFSGGDMLAEMVSLSGAGQLVALG
                     NLTLKLTRGLTAQGVIAANKQLSVSSQGDITNGATLQGNGITLNAAGRLTNNGQLTAG
                     NGTTALSGSGIAMNASGSLQAGGDVSLTSRGDITLDAFTGTTGSLMLTAAGAVINTAL
                     LYAGNNLSLFASTIRNHHGDMLAGDSLVMQKDVSGAANAEVINTSGNIETTRGDITIR
                     TGHLLNQREGINETKSYIPVENVAVPDGANSVSVRVGDLGEDGWGYYVKSWSGTAGGG
                     FDAWAVPTEKGATRKFLTGTTRVDVGATGGDARISAGNNLLIDADKLDNTGSHLLASG
                     FVSLSGSQLNNQSFFGYTQDEYNVYRYYGKLAMIPNDGHLQYGDASADDRVTFTLSGA
                     PEYVTRDTGQALRAVIQAGKNVTAVFSSDISNTSTTSNAGRITNTLAAPEINTPAEKN
                     ISPGMAQLAPDGTETLTVTAPDWTDTITRLTIGSGTDLASGIVEGNYPLPSGNNGYFV
                     PSTDPDSPYLITVNPKLDGLGKVDSSLFTGLYDLLRMHPGQAPRETDPAYTDEKQFPG
                     SSYFLDRLGLKPEKDYRFLGDAAFDTRYVSNYMLNQIGGRYINGVGSDTDQMRYLMDN
                     AARAQKALGLKFGVALTADQVAALDQSILWYKAVTIKGQTVMVPEVYLSPKDVTLQNG
                     SIISGQNVHLAGGNVTNSGSTLMAQNNLTIDSADSLGNLESGLINAGGALGLKAMGDI
                     NNISATITGKTVRLESLAGNVNNLTRYSHWQLDAPEDSLALKHTYTGSIASVSAMDSL
                     DIRADKNISVTGAEISAGDRAALIAGNDLSLNAIDRVSSRRHANSESHQRSAGLTTIT
                     AGDSVMLSAGRDVSSQGAGIAAEDNITVRAGRDVNLLAEESVTGSSSYSKKKTVIDET
                     VRQQGTEIASGGDTTITAGRDITAVASSVTATGNISVNAGRDVALTTATESDYHYLET
                     KKKSGGFLSKKTTHTISEDSATREAGSLLSGNRVTVNAGDNLTVEGSDVVADRDVSLA
                     AGNHVDVLAVTSTDTSWRFKETKKSGLMGTGGIGFTIGSSKTTHDRREAGTTQSQSAS
                     TIGSTAGNVSITAGKQAHISGSDVIANRDISITGDSVVVDPGHDRRTVDEKFEQKKSG
                     LTVALSGTVGSAINNAVTSAQETKESSDSRLKALQATKTALSGVQAGQAAAMATATGD
                     PNATGVSLSLTTQKSKSQQHSESDTVSGSTLNAGNNLSVVATGKNRGDNRGDIVIAGS
                     QLKAGGNTSLDAANDILLSGAANTQKTTGRNSSSGGGVGVSIGAGGNGAGISVFASVN
                     AAKGSEKGNGTEWTETTTDSGKTVTINSGRDTVLNGAQVNGNRIIADVGHDLLISSQQ
                     DTSKYDSKQTSVAAGGSFTFGSMTGSGYIAASRDKMKSRFDSVAEQTGMFAGDGGFDI
                     TVGRHTQLDGAVIASTATPDKNHLDTGTLGFSDLHNEADYKVSHSGISLSGGGSFGDK
                     FQGNMPGGMISAGGHSGHAEGTTQAAVAEGTITIRDRDNQKQNPADLSRDPAHANDSI
                     SPIFDKEKEQRRLQTVGLISDIGSQVADIARTQGELNALKAAKEATGETLPANATEKQ
                     RQEYLAKLRDTPEYKKEQEKYGTGSEIQRGIQAATAALQGLAGGNLAGALAGASAPEL
                     AHLLKSTEKDPVVNAIAHAILGGAVAAMQGNNVAAGAAGAATGEMAARAIAGMLYPGV
                     KQSDLSEEQKQTISTLATVSAGLAGGLTGNSSASAAVGAQSGKNAVDNNYLSVSEKTE
                     LEIAKQTLKNSKNPAEREKAQQKYDALLEKDIASDKEVIAACGNGNAGSSACASARLK
                     VIASKEGYEDGPYNSKYSQQYADAYGQIVNLLDITSVDVQNQQQVKDAMVSYFMATLG
                     VDQKTAQGYVETTQGLEIAAASMTPLFGQAVANKITALVDKANKYPSGIGFKINQPEH
                     LAQLDGYSQKKGISGAHNADVFNKAVVDNGVKIISETPTGVRGITQVQYEIPTKDAAG
                     NTTGNYKGNGAKPFEKTIYDPKIFTDEKMLQLGQEAAAIGYSNAIKNGLQAYDAKAGG
                     VTFRVYIDQKTGIVSNFHPK"
     gene            complement(10880..12658)
                     /locus_tag="ACHKIR_003118"
                     /pseudo
     CDS             complement(10880..12658)
                     /locus_tag="ACHKIR_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231833.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0046930 - pore complex [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
     gene            13158..13547
                     /locus_tag="ACHKIR_003119"
     CDS             13158..13547
                     /locus_tag="ACHKIR_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9990815.1"
                     /translation="MKRYIKWLAITIFISMLSACARTAPVQQISTTVSVGHTQEQVKN
                     AILKAGAQRKWIMTQVSPGVIKARYQTRNHVAEVRITYTATYYNIKYDNSLNLQASDG
                     KIHKNYNRWVRNLDKDIQVNLSTGATL"
     gene            13611..14669
                     /locus_tag="ACHKIR_003120"
     CDS             13611..14669
                     /locus_tag="ACHKIR_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFO9990816.1"
                     /translation="MYEQDTLSTLDAITEAQRIAFAPMLFQAALCLRNAGILDYLDQQ
                     GKQGAPLNAITEQTVLNEYAVSVLLDMGLSGRIITCKEGNYYLAKIGHYLLHDTMTRV
                     NMDFTQDVCYQGLFFLADSLNEGKPSGLKVFGDWPTIYPALSQLPDAARASWFAFDHY
                     YSDGAFNAALPYVFANNPTTLYDVGGNTGKWALRCCKYNENIAVTLLDLPQQIVLAKE
                     NIANAGFSDRIDFQAVDMLSDAPLPGEADIWWMSQFLDCFSPEQIISILSKIASVMKP
                     GAKVCIMELFWDAQRFEAASFSLNASSLYFTCMANGNSRFYSAEKFYDYLNKAGFQVA
                     ERHDNLGVGHTLLICQKK"
     gene            14710..15432
                     /locus_tag="ACHKIR_003121"
     CDS             14710..15432
                     /locus_tag="ACHKIR_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HFO9990817.1"
                     /translation="MKFALNITNWQALAPGLSDVQQWQAWSRQPWAIDPAAPLAKLSE
                     LPMMTARRLSSGSKLAVECGLAMLRRYQPDAVLYTSRHGELERNYRIVHALATEQALS
                     PTDFALSVHNSSVGNLTIAAKQPIVSSSLSAGRDSFQQGLCEVLSLLQAGYQRVLMVD
                     FDGFLPEFYHPQLPAEMPTWPYAVALMIESGDDWQCETQSAIAVNETTLPQSMLFLQH
                     YLQNAGAFSLSGERVQWRWSRR"
     gene            15429..16250
                     /locus_tag="ACHKIR_003122"
     CDS             15429..16250
                     /locus_tag="ACHKIR_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFO9990818.1"
                     /translation="MNKLMSRLGWAWRLVMTGLCFALFGLGGLLLSVVWFNILLVLVW
                     DTSRRRRLARRSIAASFRLFLTVAKGLGVLDYRIDGAEILRQERGCLVVANHPTLIDY
                     VLLASVMPETDCLVKSALLKNPFLGGVVRAADYLINSEAETLLPRCQQRLAQGDTILI
                     FPEGTRTKPGEKMTLQRGAANIAVRCASDLRIVTIGCSEHLLDKQSKWYDVPPARPFF
                     TVEVHGRVEINKFYDATSHEPALAARQLNRHLLLQLQQGCLPLSGINNASALSGN"
     gene            16225..16482
                     /locus_tag="ACHKIR_003123"
     CDS             16225..16482
                     /locus_tag="ACHKIR_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HFO9990819.1"
                     /translation="MQALYLEIKNLIISTLNLDELTPDDIDTNAPLFGDGLGLDSIDA
                     LELGLAVKNEYGVVLSAESEEMRQHFFSVATLASFIAAQRA"
     gene            16494..16745
                     /locus_tag="ACHKIR_003124"
     CDS             16494..16745
                     /locus_tag="ACHKIR_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFO9990820.1"
                     /translation="MTDQQTIYQEVSALLVKLFEIDPQDIKPEARLYEDLELDSIDAV
                     DMIVHLQKKTGKKIKPEEFKAVRTVQDVVEAVKRLLQEG"
     gene            16750..17331
                     /locus_tag="ACHKIR_003125"
     CDS             16750..17331
                     /locus_tag="ACHKIR_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9990821.1"
                     /translation="MSGIRSLPMIKLLTGLLLLAWPFLIWLGLAHNSLHWLSPLMALL
                     LLLRLRQPRRQAGPLQAVTKIVAVVGIVLCVSSFLLKTHQLLLFYPVVVNAVMLAVFG
                     GSLRSTMPIVERLARLQEPDLPEKAVRYTRRVTQVWCLFFIFNGSIALFTTLYGNMPL
                     WTAWNGIIAYLLIGFLTAGEWLIRCQMIKRKTP"
     gene            17328..18686
                     /locus_tag="ACHKIR_003126"
     CDS             17328..18686
                     /locus_tag="ACHKIR_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HFO9990822.1"
                     /translation="MNQTLSLSQWLTASRPVTTPVAWLGEYTWTLGHLRHDVALLIDH
                     LRDQPGNRWALCFENSYLFIVALLATLHSGKIPVIPGHNRAALLNVQRSLFDCILSDK
                     RLGWNGPQFVVSTSHQMTTSTIAFDDIASDAFIELFTSGSTGQPKQIAKPVISLDQEA
                     NMLATHFADRLLGCRFVASVMPQHLYGLTFRIFLPMALGLPLHAAMLWYSEQLAALSH
                     TYRYAFVSSPAFLKRLDLHLTPPPVEMILSAGGMLPWQDVEKTSTWLNIWPDEIYGST
                     ETGILAWRYRQQDNIPWFTFSDVHLSQESEGVRVFSPLIPAEGLVLDDMLQFNENGQF
                     HLIGRRGRVVKIEEKRISLVEIEQRLLALDGIMDVAAIPLIRNGRQAIGVVVVPNKEA
                     RQIWQCSGGKTLELSWRKALLPWLEPVAVPRYWRIIDEIPVNNMNKRVYAQLQELFHD
                     TP"
     gene            18673..19026
                     /locus_tag="ACHKIR_003127"
     CDS             18673..19026
                     /locus_tag="ACHKIR_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymyristoyl-ACP dehydratase"
                     /protein_id="HFO9990823.1"
                     /translation="MIRHEIERHQVQLQEMEIVLYLDPMLYWFNGHFAVQPLLPGVAQ
                     LDWVMHYATTLLAPGWRFRSIQNVKFLAPLIPETTVTLQLTWQETSQVLTFCYQRHDG
                     DARHTASSGKIRLCR"
     gene            19017..20693
                     /locus_tag="ACHKIR_003128"
     CDS             19017..20693
                     /locus_tag="ACHKIR_003128"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFO9990824.1"
                     /translation="MSVNFSPCVLIPCYNHGAMMPGVLARLKPFNLPCIVVDDGSDAA
                     TQQQLDNLVAEQPGVTLIRLAENTGKGAAVMRGLQAAADAGFSHAVQVDADGQHAIED
                     IPKLLALAEQHPAALISGQPIYDDSIPRSRLYGRWVTHVWVWIETLSLQLKDSMCGFR
                     VYPVAPTLQLAKYATIGKRMDFDTEVMVRLYWQGNTCYFVPTRVTYPQDGLSHFDALK
                     DNVRISLMHTRLFFGMLPRIPSLLMRRSSSHWARQSEVKGLWGMRLMLLVWRLLGRTA
                     FSALLYPVVGVYWLTAARARKASQDWLARVRQHQPQAAKLNSYQHFLRFGNAMLDKIA
                     SWRGELQLGHDVLFAPGAEAALDVRDPRGKLLLASHLGDVEVCRALAKIQGYKTINAL
                     VFSENAQRFKQIMQEMAPQAGINLMPVTDIGPETAILLKEKLDNGEWVAIVGDRIAVT
                     PQRGGDWRVCWSPFMGQPAPFPQGPFILASILRCPVNLIFALRQRGKLHIHCETFADP
                     LLLPRGERQQGLQNAIDHYAARLEHYALQSPLDWFNFFDFWQLPEIQDKE"
     gene            20697..21119
                     /locus_tag="ACHKIR_003129"
     CDS             20697..21119
                     /locus_tag="ACHKIR_003129"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFO9990825.1"
                     /translation="MLNDPRFTAEVELTIPFHDVDMMGVAWHGNYFRYFEVAREALLN
                     QFNYGYRQMKESGYLWPVVDARVKYRHALTFEQRIRVRAHIEEFENRLRIGYQIFDAE
                     TGKRATTGYTIQVAVEEQSRELCFVSPDILFERMGVKP"
     gene            21116..21721
                     /locus_tag="ACHKIR_003130"
     CDS             21116..21721
                     /locus_tag="ACHKIR_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HFO9990826.1"
                     /translation="MKFLPLLALLISPFVSALTLDDLQQRFTEQPVIRAHFDQTRTIK
                     DLPQPLRSQGQMLIARDQGLLWDQTSPFPMQLLLDDKRMVQVINGQPPQIITAENNPQ
                     MFQFNHLLRALFQADRKVLEQNFRVEFADKGEGRWTLRLTPTTTPLDKIFNTIDLAGQ
                     TYLESIQLNDKQGDRTDIALTQHQLTPAQLSDDERQRFAAQ"
     gene            21690..24008
                     /locus_tag="ACHKIR_003131"
     CDS             21690..24008
                     /locus_tag="ACHKIR_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HFO9990827.1"
                     /translation="MTNANVLPPSKRPALLWGLVCLVMAVVLLILLPQSRLNSSVLAM
                     LPKQAMGDIPPALNDGFMQRLDRQLVWLVSPGKEANPRVAQEWLTLLQKSAALGDIKG
                     PMDAASQQAWGAFFWQHRNGLIDPDTRARLQNGGEAQAQWILSQLYSAFSGVSGKELQ
                     NDPLMLMRGSQLAMAKNGQRLRLMDGWLVTQDPQGNYWYLLHGELAGSSFDMQQTHQL
                     ITTLNTLEKDLKTRYPQAQLLSRGTVFYSDYASQQAKQDISTLGVATLLGVILMIVAV
                     FRSLRPLLLCVISIGIGALAGTAATLLIFGELHLMTLVMSMSVIGISADYTLYYLTER
                     MVHGNDVSPWQSLAKVRNALLLALLTTVAAYLIMMLAPFPGIRQMAIFAAVGLSASCL
                     TVLFWHPWLCRGLPVRPVPAMALMLRWLAAWRRNKKLSLGLPVALALFSLAGMSMLRV
                     DDDISQLQALPQHILAQEKAITALTGQSVDQKWFVVYGDSPQQTLGRLETFTTSLEKA
                     KKDGLISNYRTIPLNSLARQEEDLDLLKTAAPTVTKALQNAGLTAVNPDLNAMPVKVD
                     EWLASPASEGWRLLWLTLENGESGVLVPVEGVKSSALQQEIASYYPGGIAWVDRKSTF
                     DELFALYRYVLTGLLLVALAVIACGAVARLGWRKGLISLVPSVLSLGCGLAVLAMSGQ
                     AVNLFSLLALVLVLGIGINYTLFFSNPRGTPLTSLLAITLAMLTTLLTLGMLVFSATQ
                     AISSFGIVLVSGIFTAFLLSPLAMPDKKRTKK"
     gene            24005..24589
                     /locus_tag="ACHKIR_003132"
     CDS             24005..24589
                     /locus_tag="ACHKIR_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3261 domain-containing protein"
                     /protein_id="HFO9990828.1"
                     /translation="MIKSTFWRALALTATLILTGCSHSQPEQEGRPQAWLQPGTRITL
                     PAPGISPAVNSQQLLSGSFNGKTQSLLVMLNADDQKITLAGLSSVGIRLFLVTYDAKG
                     LRAEQSIVVPQLPPASQVLADVMLSHWPISAWQPQLPTGWTLRDNGNKRELRNASGKL
                     VTEIIYLNRQGKRVPISIEQHVFKYHITIQYLGD"
     gene            24591..25760
                     /locus_tag="ACHKIR_003133"
     CDS             24591..25760
                     /locus_tag="ACHKIR_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HFO9990829.1"
                     /translation="MIYISAVGMINALGNNLDEIAANLARGVAPGMRPRAGWLQGHPQ
                     AVLAGVDGELPLIPEKFAAHRSRNNQILLAALAQIQPQVDDAIAKYGRERIAIVLGTS
                     TSGLHEGDTHVNLRTNGQPSTTWHYAQQELGDPSRFLSHWLALDGPAYTLSTACSSSA
                     RAMISGRRLIEAGLVDAAIVGGADTLSRMPINGFHSLESLSPTLCQPFGRDRAGITIG
                     EGAGLMLLTREPQPIALLGVGESSDAYHISAPHPQGEGAIRAINQALTDAQLTPDDIG
                     YINLHGTATQLNDQIESMVVNALFGKRIPCSSTKHLTGHTLGAAGITEAAISMLILQR
                     DLPLPAQDFSLSPRDPTLPPCGIIEKPQPLARPVILSNSFAFGGNNASILLGRVS"
     gene            25757..26221
                     /locus_tag="ACHKIR_003134"
     CDS             25757..26221
                     /locus_tag="ACHKIR_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-fatty acyl-ACP dehydratase"
                     /protein_id="HFO9990830.1"
                     /translation="MSHYLSPGAYLPHDAPMLLLEDVVSVSDDSAVCRVTVSPSGVLA
                     PFLDPDGNLPGWFALELMAQTVGVWSGWHRHQQGKNSIELGMILGARELLCTTGILPA
                     GQTLTITVKLLMQDDRFGSFECSINDGEATGRINTFQPTAEELTTLFQQGAS"
     gene            26221..26952
                     /locus_tag="ACHKIR_003135"
     CDS             26221..26952
                     /locus_tag="ACHKIR_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase FabG2"
                     /protein_id="HFO9990831.1"
                     /translation="MSRSVLVTGASKGIGRAIACKLAADGFNIGVHYHRDATGAQETL
                     NAIVANGGNGRLLSFDVANREQCREVLEHEIAQHGAWYGVVSNAGIARDAAFPALSDD
                     DWDAVIHTNLDSFYNVIQPCIMPMIGARQGGRIITLSSVSGVMGNRGQVNYSAAKAGI
                     IGATKALAIELAKRKITVNCIAPGLIDTGMIEMEESALKEAMSMIPMKRMGQAEEVAG
                     LASYLMSDIAGYVTRQVISINGGML"
     gene            26949..28178
                     /locus_tag="ACHKIR_003136"
     CDS             26949..28178
                     /locus_tag="ACHKIR_003136"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HFO9990832.1"
                     /translation="MTRRVVITGMGGVTAFGENWQDVSARLLAYENAVRKMPEWQVYD
                     GLHTLLGAPVDDFTLPEHYTRKRIRAMGRVSQMSTRASELALEQAGLIGDPILTSGET
                     GIAYGSSTGSTGPVSEFATMLTEKHTNNITGTTYVQMMPHTTAVNTGLFFGLRGRVIP
                     TSSACTSGSQAIGYAWEAIRHGYQTVMVAGGAEELCPSEAAVFDTLFATSQHNDAPKT
                     TPSPFDENRDGLVIGEGAGTLILEELEHAKARSATIYGEIVGFATNCDAAHITQPQRE
                     TMQYCMEQSLKIAGLSAQDIGYISAHGTATDRGDMAESLATATIYGDNVPLSSLKSYF
                     GHTLGACGALEAWMSLQMMREGWFAPTLNLNKPDPNCGALDYIMHEARKVDCEFLQSN
                     NFAFGGINTSIIIKRWP"
     gene            complement(28352..28621)
                     /locus_tag="ACHKIR_003137"
                     /pseudo
     CDS             complement(28352..28621)
                     /locus_tag="ACHKIR_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001167450.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(29018..30049)
                     /locus_tag="ACHKIR_003138"
     CDS             complement(29018..30049)
                     /locus_tag="ACHKIR_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO9990833.1"
                     /translation="MEKTRKRRGTGRVTLQEVANFAGVGTMTVSRALRTPEQVSDKLR
                     EKIEQAVEELGYIPNRTAGALASGHSHTVAVLVPSLTDKASSRFMQSLQQVLNKNEFQ
                     LLLGCHEYNQLKEAEILMTLLQGNPAALVIFGSQLADKTHQILEKTNIPTINVIGSPF
                     SPAQITIETAFFEASHKLTEHLLEQGYKNIGFIGAHMDNRLQRQQLNGWHKAMLSHYQ
                     NTDLVITMPDAASLQLGRYALNEILLRQPELDAVICSHEEIALGIMFECQRRLLKIPG
                     NIAVACLDGSDNCDQTHPTLTSIRIDYKKMGIETGKLLIGLLNNNHDESEESRIVQFN
                     YQIELRQST"
     gene            30320..30763
                     /locus_tag="ACHKIR_003139"
     CDS             30320..30763
                     /locus_tag="ACHKIR_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011192680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFO9990834.1"
                     /translation="MLKNLLNTEVVQVVEQVKDWREAVAISCRPLIENGSIEPRYVDA
                     IYHSHDTIGPYYVVGPGIAMPHARPEEGANKLSLALTLIPSGVNFDADENDPVKLLIV
                     LAATDSTSHIEAISQLAKLFDNEKDIQAILTAKTTQDILSVIARY"
BASE COUNT         6865 a         8381 c         7951 g         7605 t
ORIGIN      
        1 ccaacagtaa agcgaaaaaa caatggagaa ctcgaagaag cagcatagcg tttcgtagag
       61 ttagcccttg ctaagggatg attagttagt attgtttgat ggttattgct aagtcttcca
      121 tagttggtaa aattccgaaa tgttaaactt ttgataaatg gtattaaatc aaggggttat
      181 atggaaatca aagatgtttt cggggcgcaa cccaaaagtg tgtgggagta tatctgtgag
      241 aatggtcaag gattgtatgt gccagcatat caaaggcaat attcttggga taaacctcca
      301 ggttgtgatg ccagttccac catacgaagt ttgaattcat tcgaatagtt tttacgaggt
      361 tcttttcgcc agtcctgtaa ttccatactt agatgtccgt ctatatcaga tgggcgtcta
      421 agttaccaat tctcgtctga tggctacata cggcggtcag tttacgctta cggttttgac
      481 atcaattatc tccggtaatt tgatgaatga atgtcggtaa tgctaactga ctgaaaatcg
      541 ctgtgaagat gacttccccg taacgacaca acgtatgtca acaggccgca gatgctcacg
      601 tggtttaacg ccttccccgg tgcgcttaac gacattagag atgtgccggt gattaagcgt
      661 ggggatggac attgtggatg agcctgacgg ttaaagaaag atcccggcgc agtggccgga
      721 atctttactc aggatgggat tttagacagt aatttgttta ctttttcagt atcttcaggg
      781 tgtaactgaa gatatttttc acaggccaga cgaacatatt caaaacaggt ttcttcgctt
      841 acgatcacta tatcatcttc atctggggga tagccgacag caaattcaac accttcaaaa
      901 tgttcgcttt catcataact attcatatca ggaaagttac aataaattcc atccagaata
      961 taagattctt tgttaactat attttctaca gcgtccaaga acctattttc attatacata
     1021 aggtcaaagt atgaccttat ggtccaccca ggttcagaac gttcatatgg caattcaaat
     1081 aaatatttat tcatagttac tcctatttcg ggtggaagtt acttacaatt cctgtttttt
     1141 gatcaatata tactctgaac gtaacaccac cggcttttgc atcatatgcc tgtaatccat
     1201 tttttattgc attggaataa ccaattgccg ctgcctcttg accaagttgc aacattttct
     1261 catctgtaaa tattttagga tcataaatag ttttctcaaa aggcttggcg ccatttcctt
     1321 tataattacc agtcgtattc ccagcagcat cttttgtggg gatttcatac tgaacctgag
     1381 taatacctct gactcctgtt ggtgtttcag aaataatttt cacaccatta tcaaccacag
     1441 ctttattgaa tacatcagca ttatgtgcac cgctaattcc tttcttctgc gaatatccgt
     1501 caagttgagc taagtgttcc ggctgattaa ttttaaaacc aatccctgaa ggatatttat
     1561 tcgctttatc aaccaatgca gtaattttat tagccacagc ctgaccaaat aatggtgtca
     1621 ttgaagccgc tgcaatttct aatccctgcg tcgtctcgac ataaccttgt gctgttttct
     1681 ggtcaacccc cagcgttgcc atgaagtaac tcaccatcgc atctttcacc tgctgttgat
     1741 tttgtacatc aacactggtg atatcgagga gattgactat ctgaccatac gcatcagcgt
     1801 attgctggct gtatttcgag ttataagggc catcttcgta cccttctttc gacgcaataa
     1861 ctttcagtct tgcgctggca cacgctgaac tacccgcatt accattaccg caggcagcaa
     1921 taacctcctt atcactggca atatcttttt caagcaatgc atcatatttc tgctgtgcct
     1981 tttcacgctc tgccggattt ttactgtttt taagcgtctg ttttgctatc tcaagctctg
     2041 tcttttcaga cacgctcagg tagttattat caacagcatt cttcccgctc tgtgcgccca
     2101 ctgctgctga tgcgctgctg tttccggtaa gaccgccagc cagccctgct gacacggttg
     2161 ccagcgtact tatcgtctgc ttctgctctt cactgaggtc tgactgtttc acgcccggat
     2221 acagcatgcc ggcaatcgct ctggctgcca tctctcccgt tgctgcgcct gctgcaccgg
     2281 cagcaacatt attaccctgc attgccgcta ctgcgccacc cagaatggcg tgagcaatgg
     2341 catttactac cggatctttt tctgttgatt tcagcaggtg cgccagttcc ggggccgaag
     2401 ctccggccag agcacccgct aaattccccc cagccagtcc ctgaagcgcg gctgtcgcgg
     2461 cctgtatacc ccgctgaatc tcactgccag tgccatactt ttcctgttcc tttttgtatt
     2521 ccggcgtatc acgcagtttc gcaagatatt cctgacgttg tttctctgtt gcattagcag
     2581 gaagtgtttc gccggttgct tccttcgcgg ccttcaacgc attcagttcc ccctgcgtcc
     2641 gcgcgatatc cgccacctga ctgcctatgt cactgataag ccccactgtc tgcagacgcc
     2701 tctgctcctt ctccttgtca aatatcgggc tgatactgtc attagcgtgc gcaggatcac
     2761 ggctgaggtc tgccggattc tgcttctgat tgtccctgtc ccggatggtg atagtgcctt
     2821 ctgccactgc ggcctgagtc gttccttccg catgtccgct gtgacctccg gcggatatca
     2881 tgccacccgg catgttaccc tgaaatttat ccccgaagct gccaccaccg ctcagactga
     2941 tcccactgtg actgacttta taatccgctt cgttgtgaag gtcactgaac cccagcgttc
     3001 cggtatccag gtggttttta tccggtgtgg cagtggaggc aatcaccgca ccatccagtt
     3061 gggtatgtct gcccactgtg atgtcgaagc caccatcacc ggcaaacatt ccggtttgtt
     3121 cagcaacgga gtcaaagcgg ctcttcatct tatcccggga ggcagcgatg taacctgagc
     3181 cggtcatgga gccaaaggta aaactgccgc cggcagccac gctggtctgt ttactgtcgt
     3241 acttactggt gtcctgctgg ctgctaatca gcaggtcgtg gcccacatcg gcgataatcc
     3301 tgttgccgtt gacctgagca ccgttcagta ccgtatcccg accactgttg atggtgacgg
     3361 ttttaccgct gtctgttgtg gtttcagtcc actcagtacc gttacctttc tcgctgcctt
     3421 ttgccgcatt aacgctggca aagacgctga taccggcacc gttaccaccg gcaccgatac
     3481 tgacacccac gccaccgcca ctgctgctgt tcctgcccgt tgttttttgt gtgtttgcag
     3541 cgccactcaa cagaatatca ttcgcggcat ccaggcttgt gttaccaccg gccttaagct
     3601 ggcttcctgc aatcacaata tctccgcggt tatcgcccct gtttttgccg gttgcgacaa
     3661 cagacagatt attcccggca ttcagcgtac tgccggatac tgtgtcactt tcagaatgtt
     3721 gttgtgattt cgatttctga gtggtgagcg acaggctgac tcccgtcgca ttcgggtcac
     3781 cggttgcggt ggccattgcc gcagcctgtc cggcctgcac accagacagc gctgtctttg
     3841 tggcctgcag ggctttcaga cgactgtcac tgctctcctt cgtttcctgt gcactggtga
     3901 ccgcattatt gatggcactg cccactgtgc cggaaagggc aaccgtcagc ccgcttttct
     3961 tctgctcaaa tttttcgtcc acagtacgac ggtcatgccc cgggtcaacc accacactgt
     4021 caccggtaat gctgatatcc cggttcgcaa tcacatccga accgctgata tgagcctgtt
     4081 tgcccgcggt aatactgaca ttaccggcag tggagccgat ggtactggca ctctgactct
     4141 gcgttgtccc ggcctcgcgg cggtcgtgcg ttgtcttact gctgccgatg gtgaagccga
     4201 taccgccagt acccatcaga cccgatttct tcgtttcctt aaagcgccag gacgtatctg
     4261 tactggtgac agcaagaaca tcaacatggt tacccgccgc cagtgacaca tcccggtcag
     4321 ccaccacatc cgaaccctct accgtcaggt tatcaccggc gttaacggtc acgcggttcc
     4381 ccgacagcag ggagcctgct tcacgggtgg cactgtcctc actgatggtg tgggtggttt
     4441 tcttactgag aaaacctccg ctttttttct tcgtttccag atagtgatag tcactttctg
     4501 tcgccgtggt cagggcaaca tcacgaccgg cattcacgct gatattgccg gttgcggtaa
     4561 cggatgacgc aacagcggtg atatcccgtc ctgcagtaat ggtggtgtca ccaccgctgg
     4621 cgatttccgt tccctgctgg cgaacggttt catcaatgac cgttttcttt ttcgagtatg
     4681 aactgctgcc ggtcacggac tcttccgcca gcagattaac gtcgcgcccg gcccggacag
     4741 tgatgttgtc ttccgctgcg atacctgcac cctgactgct cacatcccgg cctgcactca
     4801 gcatcacact gtcgccggca gtaatggtcg taagcccggc gctgcgctgg tgagactcac
     4861 tgtttgcatg acgtctgctg cttaccctgt caatggcatt cagactgaga tcattgccgg
     4921 caataagtgc tgcgcggtct ccggcactga tttctgcccc tgtgaccgaa atgtttttat
     4981 ctgcccggat gtccagacta tccatggcac tgacagaggc aatgcttccc gtataagtgt
     5041 gtttcagggc gaggctgtct tccggtgcat caagctgcca gtgtgagtac cgggtgagat
     5101 tattgacatt gccggcgaga ctctccagcc ggacagtctt accggtaatc gtggcactga
     5161 tattgttgat atcgcccatc gctttcagtc cgagagcgcc accggcattt ataagcccgg
     5221 actcaaggtt acccagactg tcagcgctgt cgatggtgag attattctgc gccatcaggg
     5281 tactgccact gttagtgaca ttccctcctg ccaggtgcac attctgaccg ctgataatac
     5341 tgccgttctg cagcgtcaca tctttcggtg acagatacac ctccggcacc atcaccgtct
     5401 gaccctttat cgtgacggct ttataccaca gaatactttg gtcgagagcc gccacctggt
     5461 ctgctgtcag tgccacacca aacttcagac cgagcgcctt ctgtgcacgg gcagcgttat
     5521 ccatcaggta gcgcatctgg tcggtgtctg aaccaacccc gttgatatag cgccccccga
     5581 tctggttcag catatagttc gatacatagc gggtatcaaa agcggcatcc cccaggaagc
     5641 gataatcttt ttccggtttc agcccgagcc ggtcaagaaa atacgatgag cccgggaatt
     5701 gtttttcatc agtatacgcc gggtctgttt cacgtggtgc ctgcccgggg tgcatcctga
     5761 gcaggtcata cagtccggta aacagactgc tgtccacctt tccgagacca tccagtttcg
     5821 gattcaccgt aatcagatat ggactgtcag ggtccgtgga cgggacaaaa taaccattgt
     5881 taccggaagg caggggatag ttcccctcca cgatgccgga agccaggtcc gtgccactgc
     5941 cgatcgtcag acgggtaatg gtgtcagtcc agtccggggc tgtgacggtc agcgtctctg
     6001 tgccatccgg cgccagttga gccattcccg ggctgatatt tttctctgcc ggcgtgttaa
     6061 tttcaggtgc cgcaagggta tttgtgatcc gtcctgcatt tgaggtggtg gatgtattac
     6121 tgatatcact ggaaaaaacg gcagtgacat ttttgcctgc ctgaatcacg gctctcagag
     6181 cctgaccggt gtctctggtg acatattccg gagctccgga gagagtaaag gtaacgcgat
     6241 catcggcaga agcatctcca tactgcagat gcccgtcgtt ggggatcatg gccagtttgc
     6301 cgtaatagcg atacacattg tattcatcct gtgtatagcc aaagaaggac tgattattca
     6361 gttgtgaacc cgacaggctg acaaacccgc tggcaagaag atgacttccc gtattatcca
     6421 gtttgtccgc gtcaatcagc agattattcc cggccgaaat tctggcatcc ccccctgttg
     6481 caccaacatc gacgcgtgtt gtgccagtca gaaatttccg ggtggctccc ttttcagtcg
     6541 gaacggccca tgcgtcaaaa ccaccaccgg cagtaccgct ccaggacttc acataatatc
     6601 cccatccatc ttcacctaaa tcgcccactc tgactgaaac agaatttgca ccatcaggta
     6661 ctgcgacatt ctcaaccgga atgtatgatt ttgtctcatt tatgccctcc cgctgattca
     6721 gtaaatgtcc ggttctgatg gtgatatcac cgcgggtggt ttcgatattc ccggaggtat
     6781 taatcacttc agcattcgcg gcaccactga catctttctg catcaccagg ctgtcacccg
     6841 ccagcatatc gccgtgatgg ttccggatgg tgctggcaaa cagcgagagg ttattgcccg
     6901 catacaacag tgcggtgtta atgactgccc cggcggccgt taacatcaga ctacccgtcg
     6961 tgccggtaaa agcgtcgaga gtgatatctc ccctgctggt caggctgacg tccccgcctg
     7021 cctgcaggct gccgctggca ttcatcgcaa tcccgctgcc gctcagtgcc gtggtgccgt
     7081 tgccggcggt cagctggcca ttgttggtca gtctgcctgc agcattcagc gtgataccgt
     7141 tgccctgcaa tgtggcgcca ttggtaatat ccccctggct gctgacggag agttgtttat
     7201 ttgcggcgat aacgccctgt gcggtcaaac cacgggtgag cttcagcgtc aggttaccga
     7261 gtgcaaccag ctggcccgcg ccgctgagcg aaaccatttc agcaagcata tcaccgccgc
     7321 tgaacacctt cccgccggcg ttgttcaggt tgttcccttt tattgtcagg ccctgattac
     7381 ccagcagcgt gccgctgttg gtgatggtct gcgtgttgac gttcagttgt gcggcctgga
     7441 acagaccacg gttggtcaga ctgttacctg tgagcgttgc ctgattcgcc gccatcacag
     7501 tgccattacc ggcaagcttc gccacgttta gcagcaggtc agtggcctgt agccagccac
     7561 tgccactgta gtccggcgtg gtgaacgtca gctcaccgcc ggaaagaatc ttgccactgt
     7621 tgtgggcctg cgttgccgcg ctgagcgtgg ttttaccgtt gccctgaatg gtgccgtcat
     7681 tggtcaacgc gggtgcggaa agcgctgata cgttctggct gagaagcact ccgctggtct
     7741 ggttggtcag ggcattcagc aatgtaagag tcagcccgct ctcgccctga acacgacctg
     7801 agttggccag cgaactggcg gtgattcggt tatccgcccc ctgaagctgc ccactattgc
     7861 tgatgtctct ggcgttgacg tttaatgcac cgcccgtcag cattttaccg tgctgttgca
     7921 gattaccact gagtgtcacg ttcaggctgt taaccccact gatttcgcca ctgttgctga
     7981 gcgaatcgcc attcacttcc agcgtcttcg cgctgattaa tccctggtta cccagcactt
     8041 tgccgctcag acgggcatcg cccagggcgg taattttgct gccggtggcg gcaataacat
     8101 cgccactcag ggtcagagcc aaatccagcg cactctgaac agcaccgctg ctggtcaggc
     8161 tgctggcatc cagagtgatg ttctgcccct gaatccggcc gctgttggtc acatctcctg
     8221 cgctcagcac cagcgagttc tggctcagca gcgcgccact gttggtaagc ccgtccgcgc
     8281 tgtgcagggt cagaccgctc agccccgtga ccgtaccgct gttgtcagtg cttctgtggt
     8341 gaagcgtgac atggttaccc tggacagtgc cgctgttatc cagtgagttt ccatcaaggg
     8401 agagcgtgcc ggccgaaagc agactgcccc ggttgtccgt ggtggctgcg ttcagcgtgg
     8461 tgtcaccctg gctcatgata tcgccggtac tggtcaactg accggttacc gaagcagtaa
     8521 ggttaccggt tgccagcacg gaaccacggt tcgcccatct gtccgcctgc acggtgagat
     8581 tctgtccctg cgtggtccct gcggtattca gtgttttacc ccggagggag aggtcgcccg
     8641 ccgtcagcca gcgtccgtga cgaccctgtg agagggtgtc gcctgcaagc gccagtgcag
     8701 cggcgccctg caacaggcca tcgccatcca gcgtggtcgc cctgacgttc agcgtgtcag
     8761 cggtgatttt acccggattg ctgagggagg cagtgtttaa cgttaaacca ttatcaccgg
     8821 tgataagccc gctgttgcgg atgtccggta tatccagcgt caggtctgca gcactgtaca
     8881 gcgtgccgtt ctgctgatta tcaagcctct gtgtgttaac ggtgagtgag gcctccccct
     8941 gcaacagacc gctgttggtc agggtctgtg actgtgtatt cagggcggaa gcaacaagta
     9001 cgccgctgct ggtcagttcc ggcgcactga ggctgagcga cggggcgctg cttttcccgc
     9061 tgtgggtgag cttttcactg gcgttcaggg tgaggatatc tctggcagcc tgtgttccgt
     9121 caagctgtgc gttctgcaca tccacgctga cactgttgcc ctgcgtctgc gcagtggtcg
     9181 tggtcttcag actgttgcct tttacagaca ccgtgccacc cgcactggtt tcacccgcca
     9241 gtgtggaaga cgcgctgttc agcatgaggt taccgtcact gtttgtgacc gatttttctg
     9301 tggtggtcag tgtgtctgca gaagccacaa gttcctttgc cccggagagg gtaccgctgt
     9361 tgtttatctg cgtggcactg agcgtcagca cgtcatcgct gatgagtctg ccgcggtttt
     9421 cgagtgtacc gctgacggta acgcgggcac tgtcttttgc accggcatca ccggacagtg
     9481 tggcattgcg ggcgctgata tcgagtgtgc tgccactttt aatactgccg gtgctggtca
     9541 ggtcagtggc tgtgactttc aggccgttcc cctggacggc accggtatta ctgaatgtcc
     9601 cggttttcac cgtggcatcg cgcccggcaa ccagtgtccc gccgttgttc aggctgccgg
     9661 agctgacggt gagtgttttc ccggcaacgc tacggccttt cagtgtggta tcatcggtgg
     9721 tggtaagggt gacattttca gctgcattca cggccccgga agtctggttc aggtgcagtg
     9781 cactgagcat ggcgttctgt ccggcagtca gttcaccacc gttctgtaca atctgcccgg
     9841 atgaagacag caccagattt ttatccgtca ccagacggga ctgattcagt gtcagtcccg
     9901 ttgtcccggt aacgctgaga tttcctccgg ctttgttatc ccctgaaagt gagacatccg
     9961 ttccggttac ggtggtactg ccgccggcaa ggctgttctt caggaccagt tttccggaac
    10021 tgctcagtat gatatcgccc tcgcgggcat aaaggttacc aagattaacc ccgacaccac
    10081 tttcagtgga ggtcagatga atacgcctgg cgtacattcc accgagagcg ccggtatcaa
    10141 cggcaacttt cggcacatta ccttcgccct tcagggcact gacgcgacca tctgcagtta
    10201 tccggttagc gcctgcagtg acagttaaat ccttcgcatg aagcgcggca ttcacttccg
    10261 ttgcacgggc aataatggat acggcatcgc tccaggtgcc gtccaggccc gcgccattga
    10321 tggtgatact gccttcagtc acctccagcg cctgcaggct gccgtcggca ttcatcacag
    10381 gtttgcctgt ggtgagcgtc gcgtgcggcg tgttgataaa accacagccg tcacaggtga
    10441 taccatacgg gttggcaacc atcacattcg ccgctttgcc ggccacttcc gtatagccct
    10501 gcagcaatga acggttaccg ccagtcactt cgttgatgat acccttcgct tccccgcccg
    10561 ctttcaggtt cgggttattc tgtatcagtc caccaagctg cgtcggatta agcttaccgg
    10621 tggcattatt gagaatcagc ccttccttcc cgacgttgta atccgtaaac cggttatgcg
    10681 aaatcccggc cccgttcggc gtggcaatgt tcacgaccgg cacaccattt gccgctttat
    10741 ccattccggc cccgttttgt ggggtgatga cggcccccac agccggtaac aacggctgcc
    10801 cggcgataat cgtactgata aggtaactca gcaggcggta agtgaagcga acgggaggct
    10861 gatgcatggt aataatccct taaaacgcga cggcaacgcg ccagtaaacc gtgagatggt
    10921 caggggcaag ccagtccggg taaaccagag gcagtccggc agtgaacgaa ccggaaacat
    10981 ggccactggt ggtgctgagc ccggcagcag caccccacag cgtgccggac gagtacaggt
    11041 catctctgtc agagtgcagc cagccgccgt ccagtgcagt cactgcacgg actgtcccca
    11101 catatggcag ggagaacaga gaccaggaca gctcatttcg cagataaccg ccgttattac
    11161 cggagatata ctgctcctta aagccacgca ctgaactctc acccccgagg ctcagttgtt
    11221 ccacaccatg aagacggtcc ggtgaccact gggcataagc gctggtcagc caccacaccc
    11281 tgtccgtgac ggggcgctga aaactggcgc ttaatgacca tttccggaac tgatttacgg
    11341 gcaggtctcc ccttttcccg tggtcgcttt ctgcgccgaa ccagggcatc ccccgtgtga
    11401 ataccggatt cagtgttccg acacccccca gaaacttgtg tgtgtgattc agcccgacag
    11461 aaaatgaagt gagtttacgg ctgctgccct gaagcagaac atcatccaga taattgtgaa
    11521 taatgcggtg ctgcagacct ccggtcagtg ctgtcttcat gtccccgtta cggaacagga
    11581 catgcgacag tcccagccgg tgagtctgca ggtctcccgt ggaacgccac cgccagcccc
    11641 ggttatcaat ggtgctgagg tagtcactcc atgaatacgt gtaatccacc agggtatagc
    11701 catacggcag actgacaccg gcggcaaaat tcctcgcatc atgtgacacc gaaaagtcac
    11761 tgctccgtcc cccgctgaca aaccagttgt cagccagccc cagcggatta ttaaaggaaa
    11821 ggacaccatt taactgcccc gtaccggtat tcttctgtcc gctgttgtcg atgcccacgc
    11881 tccctgtgac aggccattcc ggcgatgccg tcagtgtcac caccgaccat ccctcacggt
    11941 caccgggcga tatttaaatc tgtaccggct ccgtacgcag acggttaatc tgctccatcc
    12001 cctgctcaat atcccgcagg ttcagaacct tcccctccat tcccgggaaa accatcttca
    12061 gggtgcggcc aggaaggtca gcgccttccg cccggatttg ctgcagcctg ccttccatga
    12121 ccgttatgtg cagtacgccc cctgaaaggt cctgctctgt cagaaaggcc cggctggtga
    12181 tatatccccg gcgtatatag ccgtccgtca cggcatccgt gaccgcggtc agccccgtga
    12241 tattcagaca ctgattcacc cacggtgcca ccagtctgtc ggtttctgca gacgtcagtc
    12301 gggtggcccc actgacaaca atgcgtgaca ccgtaaagca gggaccttca gtaccggcag
    12361 acatttccgg agacggtatg acagtcagcg gcgcactgcg ctccagcgca tcacgctgac
    12421 gctggttttc atccagcaac tgctgctgtt gctgctgaat tgcactgcgg tcacccggag
    12481 acagcatggc tgccggagac gaaaatgcgg ggataaaaaa accagaaaaa acggagaggg
    12541 agagataaca tatccccgaa gaggcgcggg cgcgccggaa tatatcataa caggggacag
    12601 acacagcccg gaataaaaga gacgttttcg tcagaatgtt gttctgccgg tgctgcatgt
    12661 tctccccgta ttctggcctg tgtaatgtca gtgtaaattc atgtcaaaaa tgtgtcagcg
    12721 ggaaatttac acctgaaaaa gtctgtcatg aaacagcttt atgacaagat atcaaaaaaa
    12781 atgtcaaaaa cgtgatcttt cttcagcttt tcagaatatt gagagcctag aggtcaatat
    12841 aaattctggg acacatatgc atttaccatt aaaaccggca caacaggctg ttcagtctca
    12901 atccatgctt tctgttttat cgatctcaga atatcggcag acaaataaaa aaggaatgtc
    12961 gagagagcta actctagatc actctccacc tcacaagcca tttacaaaaa tgatacaggc
    13021 atatctttgg tccctgccac aaccagacat tgacaatcca atataacccc aaatatattt
    13081 cggatacgaa gcttgggatt tatacagaca tttactattt aattataaaa tttgtattac
    13141 atacataaga ggcactaatg aaacgttata taaaatggct tgccatcaca atttttatca
    13201 gtatgttgag tgcctgtgcc cgtacggctc cagtgcaaca gataagcacc actgtcagtg
    13261 tgggtcatac tcaggagcag gttaaaaatg ccattttgaa agcaggtgca cagcgcaagt
    13321 ggattatgac gcaagtgtcc cctggagtta ttaaagctcg ctatcaaaca cgaaatcacg
    13381 ttgctgaggt tcgcattaca tatacagcta cttactataa catcaaatat gacaatagcc
    13441 tgaatctgca ggcttctgat ggaaaaattc ataaaaacta taaccgttgg gtgcgtaacc
    13501 tggataaaga tatacaggtt aacttgtcta caggagcaac gttatagtga tgctgatatc
    13561 gggctggaat agcccgatat cattaaactt ctctataaga atggttatat atgtacgaac
    13621 aggacacact cagcacactg gatgccatca cggaggcaca acggattgct tttgctccta
    13681 tgcttttcca ggcagctttg tgcctgcgca atgcaggaat attagattat ttggatcaac
    13741 agggtaaaca gggagctccc cttaacgcga ttactgaaca gacagtcctg aatgaatatg
    13801 ccgtgagtgt gttactggat atggggttaa gcgggcgaat tattacctgt aaagagggaa
    13861 attattacct ggcgaaaatc ggtcattacc tcctgcatga cactatgact cgcgtgaata
    13921 tggatttcac ccaggatgtc tgttaccagg ggcttttctt ccttgccgat tcactcaatg
    13981 aaggaaaacc ttccgggtta aaagtatttg gcgactggcc gacgatatat ccggcacttt
    14041 cgcaattacc agatgctgct cgtgctagct ggtttgcatt tgatcattac tattcagatg
    14101 gcgcatttaa cgccgcactg ccttatgtat tcgccaacaa ccccacaacc ttgtacgatg
    14161 tgggcggcaa tacgggtaag tgggctttgc gctgctgcaa atacaatgaa aacatcgccg
    14221 ttacgttatt agatctgcca caacaaatcg tactggcgaa agaaaatatc gcgaatgcag
    14281 gtttttctga tcgcatagat ttccaagcgg tggatatgct aagcgatgcc ccgttaccag
    14341 gcgaggctga tatctggtgg atgagccaat ttttagactg tttttcaccc gaacaaataa
    14401 ttagcatact cagcaaaatc gccagcgtaa tgaagcctgg cgcaaaagtt tgcatcatgg
    14461 agttgttctg ggatgcgcag cggtttgaag cagcatcgtt tagcctgaac gcgtcctcac
    14521 tttattttac ctgcatggca aacggtaaca gccgtttcta tagtgcagag aaattttacg
    14581 actatctgaa taaagcagga ttccaggtag cagagcgcca cgataattta ggcgttggac
    14641 atacactttt gatatgccaa aagaaataat cgtcacttaa taattttggt ttaccgcggg
    14701 cacgcgttga tgaaatttgc attaaatatt accaactggc aggcgctggc gcctggactt
    14761 agcgatgtgc agcaatggca agcatggtcg cgccagccgt gggcgattga ccctgccgcg
    14821 ccgctggcaa aattaagtga actgccaatg atgactgccc gtcgtctcag ttcaggcagc
    14881 aaactggctg ttgaatgcgg tctggcaatg ctacgccgct atcagcctga cgccgttttg
    14941 tataccagtc gtcatggcga actggagcgt aattaccgca ttgttcatgc actggcaacc
    15001 gaacaggcgc tttctccgac ggacttcgcg ctttccgtac acaattcttc cgtgggtaac
    15061 ctgaccattg cggccaaaca gccgatcgtt tcgtcatcgc tttcagctgg tcgcgacagt
    15121 ttccagcaag gtttgtgtga agtgcttagc ctgctacaag cgggatacca gcgcgtgttg
    15181 atggtcgatt ttgacggttt tctgcccgag ttttatcacc cacaactgcc tgcagaaatg
    15241 ccgacctggc cgtacgccgt cgcgctgatg attgaatctg gcgatgactg gcaatgtgaa
    15301 acgcagtcag ccattgcggt aaatgaaaca acactgccgc aaagcatgtt gtttttacag
    15361 cactatttac aaaatgcagg cgcattttca ctgtctggcg agcgcgtgca atggcgctgg
    15421 agccgcagat gaataagctg atgtcgcgcc tgggatgggc atggcgactg gtcatgaccg
    15481 ggttatgttt tgccctgttt ggtctcggcg gcctgttgct ttcagtcgta tggtttaaca
    15541 tattgttagt tttggtatgg gatacttccc gccgccgtcg tttggcgcgt cgcagcattg
    15601 ccgccagttt tcgcctgttt ttaactgtcg cgaaagggct tggcgtgctg gattatcgta
    15661 ttgatggcgc tgaaatttta cgtcaggaac gcggttgcct cgtcgtagcc aatcatccca
    15721 cgctgatcga ttacgtgctg ctggcgtcag tcatgccaga aactgactgc ctggtgaaaa
    15781 gcgcgctgtt aaaaaatcct tttcttggcg gcgtcgtgcg ggcggcagat tatttaatca
    15841 acagcgaagc tgaaaccctg ttaccccgct gccaacagcg actggcacag ggtgacacta
    15901 tcctgatttt ccccgaaggc acacgaacga aacccggtga aaaaatgaca ttacagcgcg
    15961 gtgcagcgaa tattgccgtg cgctgcgcca gcgatttacg catcgtgaca atcggctgta
    16021 gtgagcatct gctggataaa caaagtaaat ggtatgacgt tccgcctgcc aggccgtttt
    16081 ttacggttga agtccatggg cgggtagaaa tcaataaatt ttacgatgca acttcacatg
    16141 agccggcgct ggctgcaagg cagttaaacc gacatctttt gcttcaatta caacaaggtt
    16201 gtttacctct ctcaggaata aataatgcaa gcgctttatc tggaaattaa aaatctcatc
    16261 atcagcaccc tcaatctgga cgagttgact cctgatgata ttgataccaa tgcgccactg
    16321 tttggcgacg gtttagggct ggactctatc gatgcactgg aactggggct ggcagtaaaa
    16381 aatgagtacg gcgtcgtgct ctctgcggaa agcgaagaaa tgcgtcagca ctttttctcc
    16441 gtcgccactc tggcctcttt cattgctgcg caacgtgcct gaggaaagcc atcatgacag
    16501 accaacaaac catttatcag gaagtctcag ccctgctggt taagctgttt gaaatcgatc
    16561 cgcaggacat caaacctgaa gcgcgcctgt acgaagatct ggaactggac agcatcgacg
    16621 ccgttgacat gattgtgcac ctgcaaaaga aaaccggtaa gaaaatcaag ccggaagagt
    16681 ttaaagcagt gcgtacagtc caggatgttg tagaggctgt aaaacgcctg ctacaagaag
    16741 gctaatttga tgtcgggcat tcgctcgcta ccgatgatta agttgctgac cggattattg
    16801 ctgctcgcct ggcctttttt aatctggctc ggtttggcgc ataacagcct gcactggctg
    16861 tcgccgctga tggcgttgct gcttttattg cgcctgcgcc agccccgtcg tcaggcaggg
    16921 ccgctacaag ccgtcacaaa aatcgtcgct gttgtgggca ttgtattgtg tgtctccagt
    16981 tttctgttga aaacgcacca gttactgctg ttttacccgg tagtggttaa cgctgtcatg
    17041 ctggcggttt ttggcggttc gctgcggtcg acaatgccga tagttgagcg gctggcacgt
    17101 ttgcaggaac cagacctccc ggaaaaggcc gtgcgatata cccgccgcgt aacgcaggtc
    17161 tggtgcctgt tttttatttt caacggcagt attgcgttat ttaccacctt gtatggcaac
    17221 atgcctttgt ggacagcctg gaacggtatc attgcttacc ttctgatagg tttcctgact
    17281 gcaggggaat ggctgatacg ttgtcagatg atcaaacgga aaacaccatg aatcagactc
    17341 tctcactttc acagtggttg acagcctctc gccctgtaac aactcctgtt gcctggcttg
    17401 gggaatatac ctggacactg gggcatctgc ggcatgatgt agcgcttttg atcgaccatt
    17461 tacgcgacca gccaggcaat cgctgggcac tttgttttga aaacagctat ctttttattg
    17521 tagcgttgtt ggccacgcta cactcaggta aaatcccagt aatcccgggc cataatcgcg
    17581 ccgctttgtt aaatgtacaa cgctccttgt ttgattgcat cctgagtgat aaaaggctgg
    17641 gctggaacgg tccacagttt gttgtaagca catctcacca gatgacgacc agcacgatag
    17701 cctttgatga tattgcttct gacgcgttta ttgaactttt cacatcgggt tcaaccggac
    17761 aaccaaaaca aattgcaaaa cctgttatca gccttgatca agaagcaaac atgctggcaa
    17821 ctcactttgc agaccgacta ctgggctgtc gttttgtagc atccgtgatg cctcagcacc
    17881 tgtatgggct gactttccgt atttttttac ctatggcact tggcctgcca ttgcatgccg
    17941 caatgctctg gtattctgag caacttgccg cattaagcca tacctaccgc tatgcctttg
    18001 tcagcagccc ggcgttcctt aaacgactcg accttcatct gacgcctccc cccgtggaaa
    18061 tgattctctc tgccggcggt atgctaccat ggcaggatgt agagaaaact tccacctggc
    18121 tgaatatctg gcccgatgaa atctatggta gtaccgaaac aggtattctc gcatggcgtt
    18181 accgtcaaca ggataatatc ccctggttta ctttttctga tgttcatctt tcacaggaaa
    18241 gtgagggggt tcgtgtcttt tcgccactca tccctgctga aggattggtg cttgacgata
    18301 tgttgcaatt caatgaaaac ggtcagttcc acctgatcgg acgacgtggt cgagtggtaa
    18361 aaatagagga gaagcggata tcactggttg aaatcgaaca acggcttctg gcacttgacg
    18421 gtattatgga tgtcgctgca ataccattaa tacgcaatgg ccgccaggct atcggtgtcg
    18481 tggttgtacc aaataaagag gcccgtcaaa tatggcaatg ctccggaggg aaaactctgg
    18541 agttgtcctg gcgcaaagca ctattaccat ggcttgaacc tgttgcagta cctcgttact
    18601 ggcgcattat tgatgaaata ccagtaaaca atatgaataa gcgtgtctat gcacaattac
    18661 aggagttatt tcatgatacg ccatgaaatt gagcgccatc aggttcaact gcaggagatg
    18721 gaaatcgttc tataccttga cccgatgctc tactggttta atggtcattt tgccgtacag
    18781 cctctgttac caggcgtggc ccagttggac tgggtaatgc attatgctac cacgctgctt
    18841 gctccgggct ggcgttttcg tagtatccag aatgttaaat ttctggcgcc acttatacct
    18901 gagacgactg ttaccctgca attaacatgg caggaaacaa gtcaggtact cactttctgc
    18961 tatcagcgtc acgacggtga cgcccgccac accgccagta gcgggaagat ccgcctatgt
    19021 cggtaaactt ttctccctgc gtgttgatcc cctgctacaa ccacggtgcg atgatgccgg
    19081 gcgtgctggc gcgtcttaag ccatttaatc ttccctgtat tgtggtggat gacggcagcg
    19141 atgccgccac gcaacagcaa ctggataatc tggttgccga acagcctggc gtgaccttaa
    19201 ttcgcctggc agaaaacaca ggcaaaggcg cggcggtaat gcgcggctta caggcggcgg
    19261 cagacgcggg cttcagccat gcggtgcagg tggatgctga cggtcaacat gcgattgaag
    19321 atatccccaa actgctggca ctcgctgaac aacaccctgc ggcgctgatc tccggccagc
    19381 caatttacga tgactccatc ccccgctcac ggctttacgg gcgctgggtc acccacgtct
    19441 gggtatggat cgaaacgctc tccctgcaac tgaaagacag tatgtgcggc tttcgcgttt
    19501 atccggttgc gccaacgctg caactggcaa aatacgccac catcggcaag cggatggatt
    19561 tcgacactga agtgatggtg cgcctctact ggcagggaaa cacctgttat ttcgtgccaa
    19621 cccgcgtcac ttatccgcag gacgggcttt cgcattttga tgccctgaaa gataacgtcc
    19681 gcatctcgct catgcacacg cgtctgtttt tcggcatgtt gccgcgtatt ccttcactac
    19741 tgatgcgccg ctcttccagc cactgggcgc ggcagagtga agtgaaagga ttatggggaa
    19801 tgcgcctgat gctgctggtc tggcgtctgc tgggaagaac ggcgtttagc gcgctgcttt
    19861 acccggtggt gggcgtctac tggctcactg ctgctcgtgc gcgcaaagcg tcgcaagact
    19921 ggctcgcccg tgtacgacag catcaaccac aggcggcaaa actcaacagc tatcagcact
    19981 ttctacgttt cggtaatgcc atgctcgaca aaatcgccag ctggcgcggc gagctacaac
    20041 tggggcacga tgtgctgttt gcgccaggcg cagaagcggc gcttgacgtc cgcgatccgc
    20101 gcggcaaatt gctgctggcc tcgcatcttg gcgatgtgga agtgtgccgg gcgctggcaa
    20161 aaattcaggg ctacaaaacc attaacgcgc tggtgtttag cgaaaacgcc caacgcttta
    20221 aacagataat gcaggagatg gctccccagg caggcattaa cctgatgccg gtaacagata
    20281 tcggcccgga aaccgccatc ctgctgaaag agaagctgga taacggcgaa tgggtggcga
    20341 ttgtcggtga ccgcatcgcc gtcaccccgc aacgcggcgg cgactggcgc gtctgctgga
    20401 gtccgtttat gggccagcct gcgcctttcc cacaggggcc gtttattctc gcgtctattt
    20461 tgcgctgccc ggtgaatctg attttcgccc tgcgccagcg cggcaagctg catattcact
    20521 gcgaaacctt tgccgacccg ctgctgttgc cgcgtggcga acgccaacag gggctgcaaa
    20581 acgctatcga ccactacgcc gcgcgtctgg aacattacgc gctccagtcg cccctcgact
    20641 ggtttaattt tttcgatttc tggcaactgc cggaaattca ggacaaggag taaagggtgc
    20701 ttaacgatcc ccgatttacc gctgaagtcg agctgaccat tccctttcac gacgtcgata
    20761 tgatgggcgt ggcctggcac ggtaactatt ttcgctactt tgaagtggcc cgcgaggcgc
    20821 tgctcaacca gttcaattac ggctatcggc agatgaaaga gtcaggttat ttgtggccag
    20881 tggtcgatgc gcgggtgaaa tatcgccacg ccctcacctt tgagcaacgc atccgcgtgc
    20941 gtgcgcatat cgaagagttc gaaaaccgtc tgcggattgg ctatcagatt ttcgatgccg
    21001 aaacgggcaa acgcgccacc acaggataca ccattcaggt cgcagtagag gagcaaagcc
    21061 gtgaactgtg ctttgtcagc cccgatattc tgtttgaacg catgggagtc aaaccatgaa
    21121 atttttaccg ctgctggcgc tgctgattag cccgtttgtg agcgccctga ccctggacga
    21181 tcttcagcaa cgctttaccg aacaaccggt gatccgcgcc catttcgatc aaacccggac
    21241 gattaaagat ctgccgcagc cgctgcgatc tcagggtcag atgttgatcg cccgcgacca
    21301 ggggctgttg tgggatcaaa cttcaccatt ccccatgcag ctattgctcg acgataaacg
    21361 catggtgcag gtgatcaacg gccagccgcc gcaaatcatc acggcagaaa ataacccgca
    21421 gatgttccag tttaaccacc tgctgcgcgc gctgttccag gccgatcgca aagtgctgga
    21481 acaaaacttc cgcgtcgaat ttgccgataa aggcgaaggc cgctggacgc tgcgcctgac
    21541 gccgaccacc acgccgctgg ataaaatttt caacaccatc gatctcgccg ggcaaaccta
    21601 tctggagagc attcagctta atgataaaca gggcgatcgc accgatatcg ctcttaccca
    21661 acatcaactg acgccagcgc aactgagcga tgacgaacgc caacgttttg ccgcccagta
    21721 aacgccccgc gctgttatgg gggctagtct gcctggtcat ggcggtggta ttgctgatcc
    21781 tgctgccgca atcacggctg aacagtagcg tgctggctat gttacccaaa caggcgatgg
    21841 gcgatattcc cccagcgctg aatgacggct ttatgcagcg tctggaccgc caactggtgt
    21901 ggctggtcag ccccggtaaa gaggctaatc ccagggtggc tcaggaatgg ctgacgctgc
    21961 tgcaaaagtc cgctgcgctt ggcgacatta aagggccaat ggatgccgcc agccagcagg
    22021 cgtggggggc gtttttctgg cagcaccgca acggcctgat tgaccccgac acccgcgccc
    22081 gcctgcaaaa cggcggcgaa gcgcaggcac aatggatcct ctcccagctt tattctgcgt
    22141 tctccggggt aagcggcaag gagttgcaaa acgatccgct gatgttaatg cgcggctcgc
    22201 agctggcgat ggcgaaaaac ggccagcgtt tacgactgat ggacggctgg ctggtgacgc
    22261 aggatcccca gggcaactac tggtatctgc tgcacggcga actggcggga tcgtcgtttg
    22321 atatgcagca aacccaccag ctcatcacga ccctgaatac gctggaaaaa gatctgaaaa
    22381 cgcgttaccc gcaggcgcag ttgctctcgc gcggcacggt gttttacagc gattacgcca
    22441 gccaacaggc gaagcaggat atctccaccc tgggcgtggc aacgctgctg ggggtgatat
    22501 tgatgattgt ggcggtgttc cgctctttac gcccgttgct gctttgcgtg atttccatcg
    22561 gcatcggcgc gctggcggga acggccgcca cattgttgat tttcggcgaa ttacacctga
    22621 tgacgctggt gatgagcatg agcgttatcg gcatttccgc tgactacacg ctctattacc
    22681 tcaccgaacg gatggtgcac ggcaacgacg tttcgccgtg gcaaagcctg gcgaaagtgc
    22741 gcaatgcctt gctactggcg ctgctcacca ccgtggcggc atatctgatt atgatgctcg
    22801 ccccttttcc cggtattcgg caaatggcga tttttgccgc cgtcgggttg agcgcctcct
    22861 gtctgaccgt cctgttctgg catccgtggc tgtgccgtgg cctgccggta cgcccggttc
    22921 cggcgatggc gctgatgctg cgctggctgg cggcgtggcg gcgtaataaa aaactgtcgc
    22981 tgggtctgcc cgtcgcgctg gcgctgtttt cgctggcggg gatgtcaatg ctacgcgtcg
    23041 atgacgatat ctcgcagtta caggcgctgc cgcagcatat tctggcgcag gagaaagcca
    23101 ttaccgccct gaccggacaa agcgtcgatc aaaaatggtt tgtagtttac ggcgactccc
    23161 cacagcaaac cttgggacgg ctggagacat ttaccacctc gctggagaaa gcgaaaaaag
    23221 acggccttat cagcaactac cgcaccattc cgctgaactc cctggcgcgg caggaggaag
    23281 atttagacct gctgaaaacg gctgccccga cggtaacaaa agcactgcaa aatgccgggc
    23341 tgacggcggt gaacccggat cttaacgcca tgccagtgaa ggttgatgaa tggctggcaa
    23401 gccccgccag tgaaggctgg cgtctgctgt ggctgacgct ggaaaacggc gaaagcggcg
    23461 tactggtgcc ggttgaaggg gttaaaagta gcgcgttgca gcaggaaatc gccagctatt
    23521 accctggcgg cattgcctgg gttgatcgca aaagcacctt tgatgaattg ttcgcacttt
    23581 accgttatgt gttaaccggc ttgttgctgg tggcgctggc agtaattgcc tgcggcgcag
    23641 tggcccgcct cggctggcgc aaagggctta tcagcctggt gccttcggtg ctttcgctgg
    23701 gctgcggtct ggcagtgctg gcgatgagcg ggcaggcggt gaatctcttt tcgctactgg
    23761 cgctggtgct ggtgcttggc atcggcatca attacacgct gtttttcagt aatccgcgcg
    23821 gtacaccgtt aacttcccta ctggcgatca cgctggcaat gctcaccacc ttgctgacgc
    23881 tgggaatgct ggtattcagc gccacccagg ccatcagcag ttttggtatt gtgctggtga
    23941 gcggtatttt caccgccttc ctgctttcgc cgctggctat gcccgataaa aagagaacaa
    24001 aaaaatgatc aaatccacct tctggcgagc gctcgccctg accgctacgc ttatcctcac
    24061 cggctgtagc cactcgcaac cggaacagga aggccgcccg caggcgtggc tgcaacctgg
    24121 cacacgcatc acgctgcctg cgccggggat ttcacccgcc gtcaattccc agcaactctt
    24181 gagcggcagc ttcaacggca aaacccagtc gctgctagtg atgcttaacg ccgacgacca
    24241 gaaaatcacc cttgccgggc tgtcgtcggt cggcattcgc ctgtttctgg tgacctacga
    24301 tgcaaaaggt ctacgcgccg agcaatccat cgtcgtcccg cagctgccgc ccgcaagtca
    24361 ggtgctggct gacgtaatgc tcagccactg gccgattagc gcctggcaac cgcaacttcc
    24421 cactggctgg acgcttcgcg acaacggcaa caaacgcgag ctgcgtaacg ccagcggcaa
    24481 gctggtcacg gaaatcatct atctgaatcg ccagggcaaa cgcgtgccaa tcagcattga
    24541 gcagcatgtc tttaaatacc acatcaccat tcaatactta ggtgactgat atgatttata
    24601 tttccgccgt cggcatgatc aacgccctgg gaaataatct tgacgaaatc gcagccaacc
    24661 tggctcgcgg tgtcgccccc ggcatgcgtc cgcgcgcagg ctggttgcag gggcatccgc
    24721 aggcggtcct ggctggcgtg gatggcgagc ttccgcttat ccccgaaaaa ttcgctgcgc
    24781 accgctcgcg caacaatcag atcctgctgg cggcgctggc gcagatccag ccgcaggttg
    24841 atgacgctat cgcgaaatat ggccgcgagc gcatcgcgat tgtgcttggc accagcacct
    24901 ccggcctgca tgagggcgac acgcacgtta acctgcgcac caacggacag ccgagcacca
    24961 catggcacta cgcgcaacag gaactcggcg atccgtcgcg ttttctcagc cactggctgg
    25021 cgctcgacgg cccggcctat accctttcaa ccgcctgctc ttccagcgcc agagcgatga
    25081 tcagcgggcg caggcttatc gaagcggggc tggtcgatgc cgccattgtg gggggcgcag
    25141 atactctgag ccggatgccg attaacggtt ttcacagcct cgaatcgctc tcgccaacct
    25201 tatgtcagcc gtttggtcgt gaccgcgcag gtatcaccat tggcgaaggg gcgggactga
    25261 tgttattaac ccgtgaaccg cagcccatcg cgttactggg cgttggtgaa tcgagcgacg
    25321 cttaccatat ttcggccccg catccgcaag gcgaaggggc gatccgcgcc attaaccagg
    25381 cgctgaccga tgcgcagctt acgccagatg acatcggcta tatcaacctg cacggtaccg
    25441 ccacacaact caacgaccag attgaatcaa tggtggttaa cgccctgttt ggcaaacgca
    25501 taccgtgcag ctccaccaaa catttgaccg gacacacgct cggcgcggct ggcattaccg
    25561 aggcggcaat tagtatgttg attttacagc gtgatttacc gctgcccgct caggacttca
    25621 gcctgtcgcc gcgcgatccc acgctgccac cgtgtggcat tatcgaaaaa ccgcagccgc
    25681 tggcacgtcc ggtgatcctg tccaattcct tcgcctttgg cggcaataac gccagcattc
    25741 tgctcgggag ggtttcgtga gccactattt atcccccggc gcttatctgc cccacgacgc
    25801 gcccatgtta ctgctggaag acgtggtgag cgtcagcgat gacagcgcgg tgtgccgcgt
    25861 gacggtttcg cccagtggcg tactggcacc gtttctcgac ccggacggca atctccctgg
    25921 ctggtttgcc cttgaactga tggcgcaaac cgtcggcgtc tggtctggct ggcatcgcca
    25981 ccagcagggc aaaaacagta ttgagttagg gatgatactg ggcgcgcgtg aattgctttg
    26041 caccactggc attctgcccg caggccagac gctgaccatt accgtcaaac tgctgatgca
    26101 ggacgatcgc ttcggcagct ttgagtgcag catcaacgac ggcgaggcga cgggccgcat
    26161 taacaccttc cagccgaccg cggaagaact taccaccctg tttcaacaag gagcgtcctg
    26221 atgagtcgtt cagttctggt tactggtgcc agcaaaggca ttggtcgcgc catcgcctgc
    26281 aagcttgcgg cagacggctt taacattggc gttcactatc accgtgacgc cacaggcgct
    26341 caggaaacgc tgaatgccat tgtcgccaac gggggtaacg ggcgtttgct cagtttcgac
    26401 gtcgcaaacc gcgaacagtg tcgggaagtg ctggagcatg agatcgcgca acacggtgcc
    26461 tggtacggcg tggtcagtaa cgccgggatc gcccgcgatg ccgcttttcc ggctttaagc
    26521 gatgacgact gggatgcggt gatccacacc aacctcgaca gtttctacaa cgtcattcag
    26581 ccgtgcatta tgccaatgat cggcgcgcgc cagggtgggc gcatcatcac gctgtcctcg
    26641 gtttccggcg taatgggcaa tcgcggccag gtgaattaca gcgcagccaa agccggaatt
    26701 atcggcgcaa caaaagcact ggctatcgaa ctcgcaaagc gcaaaatcac cgtcaattgc
    26761 atcgcgccag ggcttattga taccgggatg atcgaaatgg aagaaagcgc tctgaaagag
    26821 gcaatgtcga tgatccccat gaaacgtatg ggccaggcgg aagaagttgc cgggcttgcc
    26881 agttatttaa tgtctgatat tgcgggttac gtcacccgcc aggttatttc catcaacgga
    26941 gggatgttat gacacgtcgc gtagtgatta caggaatggg cggcgtgact gcctttggtg
    27001 aaaactggca ggacgtttct gcacgcttac tggcgtatga aaatgcggtg cgcaaaatgc
    27061 cggagtggca ggtctacgat gggctgcaca ccctgcttgg cgcgcctgtg gatgatttca
    27121 cgctaccgga gcactacacc cgcaagcgta tccgtgcgat gggccgcgtg tcgcaaatgt
    27181 cgacccgtgc cagcgagctg gcgttagagc aggcagggtt gattggcgat ccgatattaa
    27241 ccagcggcga aacgggcatt gcgtacggtt catcgaccgg cagtaccggt ccggtgagcg
    27301 aatttgccac catgctgact gaaaagcaca cgaacaacat caccggcacc acctatgtgc
    27361 agatgatgcc gcacaccacc gctgttaata ctggcctctt tttcggcctg cgcgggcggg
    27421 tgatcccaac ctccagcgcc tgtacctccg gcagccaggc gatcggttat gcgtgggaag
    27481 ccattcgcca cggttatcaa acggtgatgg ttgccggtgg tgccgaagag ttatgccctt
    27541 cagaagcagc ggtgtttgat accctttttg ccaccagcca gcacaatgat gcgccgaaaa
    27601 ccacaccttc accgttcgat gaaaaccgcg acggactggt gattggtgaa ggcgcaggta
    27661 cgctcattct cgaagaactg gagcatgcca aagcgcgcag cgcgaccatt tacggtgaaa
    27721 tcgtcggctt tgcgactaac tgcgacgcgg cacatattac ccagccgcag cgcgaaacca
    27781 tgcagtattg tatggagcaa tcgctaaaaa tcgcagggtt aagcgcacaa gatatcggct
    27841 atatttcagc gcatggtacg gcgaccgatc gcggtgatat ggcagaaagt ctggcgacag
    27901 caaccattta cggtgataat gtgccgctct cctcgctgaa aagttatttt ggtcatacgc
    27961 ttggtgcctg tggcgcgctc gaagcctgga tgagtctgca aatgatgcgc gaaggttggt
    28021 ttgcgcctac gctaaacctg aataaaccag acccgaattg tggcgcgctg gattacatca
    28081 tgcatgaagc ccgcaaggtt gattgtgagt ttttgcagag taacaacttt gcctttggcg
    28141 gcattaatac atccatcatt attaagcgct ggccctgagt tatacaacca catggacatc
    28201 ccatgtggtg attcttcaaa aaatctgaat caatgaatat gaacctcaaa ttctcttacg
    28261 catactgaag agcattccgt ataacgattc agcagacaaa actttatcag tccacacgaa
    28321 tcaacggatg cacagatgac acgcccggtt atcaccgggc aatggttgcc ataacgggta
    28381 acataaggga ctgaacagtt gtctttagcg caacgaactg atctaacgta cctgaacgga
    28441 caacctgaaa acagccttcc atcctgcatt gtccgccagt gcttattacc aaccacgtct
    28501 gttgcagtaa agtaatcagt tgcgaacatt atttatcaat cgtgacgctg gctcgcgcat
    28561 ggcagaaaag gtcctgactg agcaacaaca ggcatcgtac tgttgctgct ccatgttgtt
    28621 tatatgaacg catcccgttt gcaaggcttc cgtgtttttg acgttgacgg tagattgctg
    28681 ctctatatcc ttattgcagc cggtgctgcg ggcctcaatg agattcgctg actctcgcct
    28741 tattcacgca acgagcttta agctcagaaa aacatccaag tttggtgagt cacagtctta
    28801 cctgtcgttg tcatcaatat aggaatggat aatctggaga gcgatactgc aggctgatat
    28861 tgcggttttg cgacaagttt ttagcatccg cttctacgcc aaatacctga agtaggaatg
    28921 gatgcgttag cagcatacat ccggaactgc gcggggaata gccggaaacg gctatcccta
    28981 ccacaatagc taactttgta atagcgctct tttaggctca tgtactctga cgtaattcta
    29041 tctggtagtt gaattgcacg atacgcgact cttctgattc atcatgatta ttattcaata
    29101 gtccaatcag taacttacct gtctctatcc ccattttttt gtagtcaata cggatggatg
    29161 tcaaagttgg gtgtgtttga tcgcaattgt ctgaaccgtc aagacaagct acagcgatat
    29221 ttccagggat ttttaacaaa cgccgctggc actcaaacat tatacccagc gcaatctctt
    29281 cgtggctgca aatcacagca tccagctctg gttgtcgcag taatatctcg tttaatgcgt
    29341 aacgccccaa ttgcagactt gcggcatccg gcatggtgat aacaagatca gtgttctggt
    29401 aatggctcag cattgcttta tgccagccgt tcagttgctg acgttgcagg cggttgtcca
    29461 tatgcgcacc gataaatccg atattcttat aaccctgctc cagcagatgt tcggtcagtt
    29521 tatgtgatgc ttcaaaaaat gctgtttcga tggtaatttg agccggactg aaaggggaac
    29581 cgataacgtt aatggtaggt atatttgttt tctcgagtat ctggtgagtt ttatcagcca
    29641 actgagaacc aaaaataact aacgccgccg ggttgccttg aagaagcgtc atcagaattt
    29701 cagcttcttt gagctgatta tactcatgac acccaagcag tagctggaat tcatttttat
    29761 taagcacctg ctgtaatgac tgcataaagc gggagctggc tttatctgtc agggaaggaa
    29821 ccagtacggc gactgtatga ctgtgaccag atgcaagggc gccagcagtt ctgttgggaa
    29881 tataacccaa ctcttctacg gcctgttcta tcttttccct cagcttgtca gaaacctgtt
    29941 ccggtgttct cagcgcgcgg gaaacggtca tggttccgac acctgcaaag ttagcgacct
    30001 cctgcagcgt cacccgtccg gtaccacggc gcttacgtgt tttttccatt tcgactccat
    30061 ggataattac aaccagctgt tttcattact tttatttcac cattactctt tccagttttt
    30121 gcccacggta gcaaatatcc tgttgcattg tatatgcagg aaccgaatat agccaaagca
    30181 tggcggcaga gaaatgatag cgctatcaca aagattggta gcgttatcag atagcgctat
    30241 caaatgcgac aatcatcaca ttaattaatg cgtaaaatat ctactctcct gttaattatc
    30301 atcgggagtt atcagtagca tgttaaaaaa tctattaaat acagaggtcg ttcaggtcgt
    30361 ggaacaggtc aaagactggc gggaagccgt ggcgatttca tgccgaccgt tgattgaaaa
    30421 cggctctatc gaaccgcgtt atgttgatgc gatctatcac tcgcacgaca ctatcggtcc
    30481 gtactatgtg gttggccctg gtattgcgat gcctcatgcg cgcccggaag agggggctaa
    30541 caagctgtca ttagcactga ccctgatccc ttcaggtgtg aattttgatg ccgatgaaaa
    30601 cgatccagtg aaattactga ttgtgctggc tgcaaccgac agtaccagcc acattgaagc
    30661 gatttctcaa ctcgccaagt tattcgataa cgaaaaagac atacaggcca ttttgacggc
    30721 caaaacgacg caggacattt tgtctgttat tgcgcgctat taatccagag gaacatttat
    30781 gaaaattaca gtggtttgcg gt
//
LOCUS       DAZGQL010000064        30495 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.064, whole genome shotgun sequence.
ACCESSION   DAZGQL010000064 DAZGQL010000000
VERSION     DAZGQL010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30495
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.064"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..570
                     /locus_tag="ACHKIR_003140"
     CDS             <1..570
                     /locus_tag="ACHKIR_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HFO9990835.1"
                     /translation="AIDKPFLLPIEDVFSISGRGTVVTGRVERGIIKVGEEVEIVGIK
                     ETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPGTIKPHTKFES
                     EVYILSKDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVTLIH
                     PIAMDDGLRFAIREGGRTVGAGVVAKVLS"
     gene            800..1183
                     /gene="secE"
                     /locus_tag="ACHKIR_003141"
     CDS             800..1183
                     /gene="secE"
                     /locus_tag="ACHKIR_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFO9990836.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            1185..1730
                     /gene="nusG"
                     /locus_tag="ACHKIR_003142"
     CDS             1185..1730
                     /gene="nusG"
                     /locus_tag="ACHKIR_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFO9990837.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEELFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1889..2317
                     /gene="rplK"
                     /locus_tag="ACHKIR_003143"
     CDS             1889..2317
                     /gene="rplK"
                     /locus_tag="ACHKIR_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFO9990838.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            2321..3025
                     /gene="rplA"
                     /locus_tag="ACHKIR_003144"
     CDS             2321..3025
                     /gene="rplA"
                     /locus_tag="ACHKIR_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFO9990839.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3438..3935
                     /gene="rplJ"
                     /locus_tag="ACHKIR_003145"
     CDS             3438..3935
                     /gene="rplJ"
                     /locus_tag="ACHKIR_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFO9990840.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            4002..4367
                     /gene="rplL"
                     /locus_tag="ACHKIR_003146"
     CDS             4002..4367
                     /gene="rplL"
                     /locus_tag="ACHKIR_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFO9990841.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4687..8715
                     /gene="rpoB"
                     /locus_tag="ACHKIR_003147"
     CDS             4687..8715
                     /gene="rpoB"
                     /locus_tag="ACHKIR_003147"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFO9990842.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8792..13015
                     /gene="rpoC"
                     /locus_tag="ACHKIR_003148"
     CDS             8792..13015
                     /gene="rpoC"
                     /locus_tag="ACHKIR_003148"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFO9990843.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(13255..14388)
                     /gene="thiH"
                     /locus_tag="ACHKIR_003149"
     CDS             complement(13255..14388)
                     /gene="thiH"
                     /locus_tag="ACHKIR_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HFO9990844.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINSKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEPLAQRAQRLTRQRFGNVVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQVKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYARHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDSWRVDCYMVAEHLLWLQQHYWQSRYSISFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRP"
     gene            complement(14385..15155)
                     /gene="thiG"
                     /locus_tag="ACHKIR_003150"
     CDS             complement(14385..15155)
                     /gene="thiG"
                     /locus_tag="ACHKIR_003150"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HFO9990845.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMMEAIRACGSQLVTLAMKR
                     VNLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIITQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            complement(15157..15357)
                     /gene="thiS"
                     /locus_tag="ACHKIR_003151"
     CDS             complement(15157..15357)
                     /gene="thiS"
                     /locus_tag="ACHKIR_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HFO9990846.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQSGAALAINQQIVPRE
                     QWAQHIVQDGDQILLFQVIAGG"
     gene            complement(15341..16096)
                     /gene="thiF"
                     /locus_tag="ACHKIR_003152"
     CDS             complement(15341..16096)
                     /gene="thiF"
                     /locus_tag="ACHKIR_003152"
                     /EC_number="2.7.7.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HFO9990847.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSHQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVARADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRNLALRRASGCPVCGGSNADPV"
     gene            complement(16089..16724)
                     /gene="thiE"
                     /locus_tag="ACHKIR_003153"
     CDS             complement(16089..16724)
                     /gene="thiE"
                     /locus_tag="ACHKIR_003153"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFO9990848.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLSAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16724..18619)
                     /gene="thiC"
                     /locus_tag="ACHKIR_003154"
     CDS             complement(16724..18619)
                     /gene="thiC"
                     /locus_tag="ACHKIR_003154"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HFO9990849.1"
                     /translation="MSVTKLTRREQRAQAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTKARLADDGLDELRFSGVLTPKRAKAGRRVTQLHYARQGIITP
                     EMEFIAIRENMGREHIRSEVLRHQHPGMSFGARLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(18700..18800)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18852..19328)
                     /gene="rsd"
                     /locus_tag="ACHKIR_003155"
     CDS             complement(18852..19328)
                     /gene="rsd"
                     /locus_tag="ACHKIR_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HFO9990850.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            19423..20196
                     /gene="nudC"
                     /locus_tag="ACHKIR_003156"
     CDS             19423..20196
                     /gene="nudC"
                     /locus_tag="ACHKIR_003156"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HFO9990851.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            20236..21300
                     /gene="hemE"
                     /locus_tag="ACHKIR_003157"
     CDS             20236..21300
                     /gene="hemE"
                     /locus_tag="ACHKIR_003157"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HFO9990852.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSAPYHL"
     gene            21310..21981
                     /gene="nfi"
                     /locus_tag="ACHKIR_003158"
     CDS             21310..21981
                     /gene="nfi"
                     /locus_tag="ACHKIR_003158"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HFO9990853.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            22024..22614
                     /gene="yjaG"
                     /locus_tag="ACHKIR_003159"
     CDS             22024..22614
                     /gene="yjaG"
                     /locus_tag="ACHKIR_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HFO9990854.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAVFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            22801..23073
                     /gene="hupA"
                     /locus_tag="ACHKIR_003160"
     CDS             22801..23073
                     /gene="hupA"
                     /locus_tag="ACHKIR_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-alpha"
                     /protein_id="HFO9990855.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            23086..23781
                     /gene="yjaH"
                     /locus_tag="ACHKIR_003161"
     CDS             23086..23781
                     /gene="yjaH"
                     /locus_tag="ACHKIR_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HFO9990856.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23783..24202)
                     /gene="zraP"
                     /locus_tag="ACHKIR_003162"
     CDS             complement(23783..24202)
                     /gene="zraP"
                     /locus_tag="ACHKIR_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HFO9990857.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGTGMGYGGCGGGGHMGMDHW"
     gene            24440..25816
                     /gene="zraS"
                     /locus_tag="ACHKIR_003163"
     CDS             24440..25816
                     /gene="zraS"
                     /locus_tag="ACHKIR_003163"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HFO9990858.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSGQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGTGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQVGKGATFTLWLPVT
                     ITRKDPQG"
     gene            25813..27138
                     /gene="zraR"
                     /locus_tag="ACHKIR_003164"
     CDS             25813..27138
                     /gene="zraR"
                     /locus_tag="ACHKIR_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HFO9990859.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSVDAETPAVSASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLANHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENTVERAVVLLTGEYISERELPLAIASTPIP
                     LVQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(27135..28424)
                     /gene="purD"
                     /locus_tag="ACHKIR_003165"
     CDS             complement(27135..28424)
                     /gene="purD"
                     /locus_tag="ACHKIR_003165"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFO9990860.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPTLQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHNIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28436..30025)
                     /gene="purH"
                     /locus_tag="ACHKIR_003166"
     CDS             complement(28436..30025)
                     /gene="purH"
                     /locus_tag="ACHKIR_003166"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFO9990861.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DANEGSLLLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAISNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTKRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7141 a         7904 c         8415 g         7035 t
ORIGIN      
        1 gcgattgaca agccgttcct gctgccgatc gaagacgtat tctccatctc cggtcgtggt
       61 accgttgtta ccggtcgtgt agaacgcggt atcatcaaag ttggtgaaga agttgaaatc
      121 gttggtatca aagagactca gaagtctacc tgtactggcg ttgaaatgtt ccgcaaactg
      181 ctggacgaag gccgtgctgg tgagaacgta ggtgttctgc tgcgtggtat caaacgtgaa
      241 gaaatcgaac gtggtcaggt actggctaag ccgggcacca tcaagccgca caccaagttc
      301 gaatctgaag tgtacattct gtccaaagat gaaggcggtc gtcatactcc gttcttcaaa
      361 ggctaccgtc cgcagttcta cttccgtact actgacgtga ctggtaccat cgaactgccg
      421 gaaggtgtag agatggtaat gccgggcgac aacatcaaaa tggttgttac cctgatccac
      481 ccgatcgcga tggacgacgg tctgcgtttc gcaatccgtg aaggcggccg taccgttggc
      541 gcgggcgttg tagcaaaagt tctgagctaa ttgccgataa catttgacgc aatgcgcact
      601 aaaagggcat catttgatgc cctttttgca cgctttcgta ccagaacctg gctcatcagt
      661 gattttcttt gtcataatca ttgctgagac aggctctgtt gagggcgtat aatccgaaaa
      721 gctaatacgc gtttcgattt ggtttgcctc gcgatcgcgg ggtgaaaatg tttgtagaat
      781 acttctgaca ggttggttta tgagtgcgaa taccgaagct caaggaagcg ggcgcggcct
      841 ggaagcgatg aagtgggtcg ttgtggtggc attgctcctg gtagcgattg tcggcaacta
      901 cctttatcgc gacattatgc tgccgctgcg tgcgctggcc gtagtaattc tgattgctgc
      961 agcgggtggt gtcgcgctgt taacgacaaa aggtaaagca accgttgctt ttgcccgtga
     1021 agcgcgtacc gaagtccgta aggtcatttg gccgactcgc caggaaacat tgcacaccac
     1081 gctgattgtg gctgcggtta ccgcagtaat gtcactgatc ctgtggggac tggatggtat
     1141 tctggttcgc ctggtatcct ttatcactgg cctgaggttc tgagatgtct gaagctccta
     1201 aaaagcgctg gtacgtcgtt caggcgtttt ccggttttga aggccgcgta gcaacgtcgc
     1261 tgcgtgagca tatcaaatta cacaacatgg aagagttgtt tggtgaagtc atggtaccaa
     1321 ccgaagaagt ggttgaaatc cgtggcggtc agcgtcgcaa aagcgaacgt aaattcttcc
     1381 ctggctacgt cctcgttcag atggtgatga acgacgcgag ctggcacctg gtgcgcagcg
     1441 taccgcgtgt gatgggcttc atcggcggta cttccgatcg tcctgcgcca atcagcgata
     1501 aagaagtcga tgcgattatg aaccgcctgc agcaggttgg tgataagccg cgtccgaaaa
     1561 cgttgtttga accgggtgaa atggtccgtg ttaatgatgg tccgttcgct gacttcaacg
     1621 gcgttgttga agaagttgat tacgagaaat ctcgtctgaa agtgtctgtt tctatcttcg
     1681 gtcgtgcgac ccctgtagag ctggacttca gccaggttga aaaagcttaa cccagcgatc
     1741 aaaaaagcgg cgatttaatc gttgcacaag gcgcgagatt ggaatacaat ttcgcgcctt
     1801 ttgtttttat gggccttgcc cgtaaaacga ttttttatat cacggggagc ctctcagagg
     1861 cgttattacc caacttgagg aatttataat ggctaagaaa gtacaagcct atgtcaagct
     1921 gcaggttgca gctggtatgg ctaacccgag tccgccagta ggtccggctc tgggtcagca
     1981 gggcgtaaac atcatggaat tctgcaaagc gttcaacgca aaaactgatt ccatcgaaaa
     2041 aggtctgccg attccggtag taatcactgt ttacgctgac cgttctttca ctttcgttac
     2101 caagactccg ccggcagcag ttctgctgaa aaaagcggct ggtatcaagt ctggttccgg
     2161 taagccgaac aaagacaaag tgggtaaaat ttcccgcgct cagctgcagg aaatcgcgca
     2221 gaccaaagct gccgacatga ctggtgccga cattgaagcg atgactcgct ccatcgaagg
     2281 tactgcacgt tccatgggcc tggtagtgga ggactaagaa atggctaaac tgaccaagcg
     2341 catgcgtgtt atccgagaga aagttgatgc aaccaaacag tacgacatca acgaagctat
     2401 cgctctgctg aaagaactgg cgactgctaa attcgtagaa agcgtggacg tagctgttaa
     2461 cctcggcatc gacgctcgta aatctgacca gaacgtacgt ggtgcaactg tactgccgca
     2521 cggtactggc cgttccgttc gcgtagccgt atttacccag ggtgcaaacg ctgaagctgc
     2581 taaagctgca ggtgctgaac tggtaggtat ggaagatctg gctgaccaga tcaagaaagg
     2641 cgaaatgaac tttgacgttg ttattgcttc tccggatgca atgcgcgttg ttggccagct
     2701 gggccaggtt ctgggtccgc gcggcctgat gccaaacccg aaagtgggta ctgtaacgcc
     2761 gaacgttgct gaagcggtta aaaacgctaa agctggccag gttcgttacc gtaacgacaa
     2821 aaacggcatc atccacacca ccatcggtaa agtggacttt gacgctgaca aactgaaaga
     2881 aaacctggaa gctctgctgg ttgcgctgaa aaaagcaaaa ccgactcagg cgaaaggcgt
     2941 gtacatcaag aaagttagca tctccaccac catgggtgca ggtgttgcag ttgaccaggc
     3001 tggcctgagc gcttctgtaa actaatgcct ttacgtgggc ggtgattttg tctacaatct
     3061 tacccccacg tataatgctt aatgcagacg tatatccgag atattcgggt tgtggcaagg
     3121 cggtaactga gtgagtcgcc aggagcatag ttaactatgt gactggtgcg aacgaaggaa
     3181 gccaacgccg ccacaagctg aatagcgacg gatagaaaag atttgttcgt tggagcctgg
     3241 cctatccagg cctccgtcga agaccgcagg agtttcgcaa gaaacttaat cccctgcgta
     3301 gacggtgaca gaacgctaag attattcttt tatattctgg cttgtttctg ctcaccgtaa
     3361 ttaagacgct ctctccgttt ggaggagtga agtgagttcc agagattttc tctggcaaac
     3421 atccaggagc aaagctaatg gctttaaatc ttcaagacaa acaagcgatt gttgctgaag
     3481 tcagcgaagt agccaaaggc gcgctgtctg cagtagttgc ggattcccgt ggcgtaactg
     3541 tagataaaat gactgaactg cgtaaagcag gtcgcgaagc tggcgtatac atgcgtgttg
     3601 ttcgtaacac cctgctgcgc cgtgctgttg aaggtactcc gttcgagtgc ctgaaagacg
     3661 cgtttgttgg tccgaccctg attgcatact ctatggaaca cccgggcgct gctgctcgtc
     3721 tgttcaaaga gttcgcgaaa gcgaatgcaa aatttgaggt caaagccgct gcctttgaag
     3781 gtgagctgat cccggcgtct cagatcgacc gcctggcaac tctgccgacc tacgaagaag
     3841 caattgcacg cctgatggca accatgaaag aagcttcggc tggcaaactg gttcgtactc
     3901 tggctgctgt acgcgatgcg aaagaagctg cttaatcgca gttatctttt taacgcattc
     3961 gcttacgtat aaacttattc tgatattcag gaacaattta aatgtctatc actaaagatc
     4021 aaatcattga agcagttgca gctatgtctg taatggacgt tgtagaactg atctctgcaa
     4081 tggaagaaaa attcggtgtt tccgctgctg ctgctgtagc tgtagctgct ggcccggttg
     4141 aagctgctga agaaaaaact gaattcgacg taattctgaa agctgctggc gctaacaaag
     4201 ttgctgttat caaagcagta cgtggcgcaa ctggcctggg tctgaaagaa gctaaagacc
     4261 tggtagaatc tgcaccggct gctctgaaag aaggcgtgag caaagacgac gcagaagcac
     4321 tgaaaaaagc tctggaagaa gctggcgctg aagttgaagt taaataagcc aacccttccg
     4381 gttgcagcct gagaaatcag gctgatggct ggtgactttt tagtcaccag cctttttgcg
     4441 ctgtaaggcg ccagtagcgt ttcacactgt ttgactactg ctgtgccttt caatgcttgt
     4501 ttctatcgac gacttaatat actgcgacag gacgtccgtt ctgtgtaaat cgcaatgaaa
     4561 tggtttaagc gtgatagcaa caggcattgc ggaaagtgtt ccattttccg gtcaacaaaa
     4621 tagtgttgca caaactgtcc gctcaatgga cagatgggtc gacttgtcag cgagctgagg
     4681 aaccctatgg tttactccta taccgagaaa aaacgtattc gtaaggattt tggtaaacgt
     4741 ccacaagttc tggatgtacc ttatctcctt tctatccagc ttgactcgtt tcagaaattt
     4801 atcgagcaag atcctgaagg gcagtatggt ctggaagctg ctttccgttc cgtattcccg
     4861 attcagagct acagcggtaa ttccgagctg caatacgtca gctaccgcct tggcgaaccg
     4921 gtgtttgacg tccaggaatg tcaaatccgt ggcgtgacct attccgcacc gctgcgcgtt
     4981 aaactgcgtc tggtgatcta tgagcgcgaa gcgccggaag gcaccgtaaa agacattaaa
     5041 gaacaagaag tctacatggg cgaaattccg ctcatgacag acaacggtac ctttgttatc
     5101 aacggtactg agcgtgttat cgtttcccag ctgcaccgta gtccgggcgt cttctttgac
     5161 tccgacaaag gtaaaaccca ctcttcgggt aaagtgctgt ataacgcgcg catcatccct
     5221 taccgtggtt cctggcttga cttcgaattc gatccgaagg acaacctgtt cgtacgtatc
     5281 gaccgtcgcc gtaaactgcc tgcgaccatc attctgcgcg ccctgaacta caccacagag
     5341 cagatcctcg acctgttctt tgaaaaagtt atctttgaaa tccgtgataa caagctgcag
     5401 atggaactgg tgccggaacg cctgcgtggt gaaaccgcat cttttgacat cgaagctaac
     5461 ggtaaagtgt acgtagaaaa aggccgccgt atcactgcgc gccacattcg ccagctggaa
     5521 aaagacgacg tcaaactgat cgaagtcccg gttgagtaca tcgcaggtaa agtggttgct
     5581 aaagactata ttgatgagtc taccggcgag ctgatctgtg ctgcgaacat ggagctgagc
     5641 ctggatctgc tggctaagct gagccagtct ggtcacaagc gtatcgaaac gctgttcacc
     5701 aacgatctgg atcacggccc atatatctct gaaaccttac gtgtcgaccc aactaacgac
     5761 cgtctgagcg cactggtaga aatctaccgc atgatgcgcc ctggcgagcc gccgactcgt
     5821 gaagcagctg aaagcctgtt cgagaacctg ttcttctccg aagaccgtta tgacttgtct
     5881 gcggttggtc gtatgaagtt caaccgttct ctgctgcgcg aagaaatcga aggttccggt
     5941 attctgagca aagacgacat cattgatgtt atgaaaaagc tcatcgatat ccgtaacggt
     6001 aaaggcgaag tcgatgatat cgaccacctc ggcaaccgtc gtatccgttc cgttggcgaa
     6061 atggcggaaa accagttccg cgttggcctg gtacgtgtag agcgtgcggt gaaagagcgt
     6121 ctgtctctgg gcgatctgga taccctgatg cctcaggata tgatcaacgc caagccgatt
     6181 tccgcagcag tgaaagagtt cttcggttcc agccagctgt ctcagtttat ggaccagaac
     6241 aacccgctgt ctgagattac gcacaaacgt cgtatctccg cactcggccc aggcggtctg
     6301 acccgtgaac gtgcaggctt cgaagttcga gacgtacacc cgactcacta cggtcgcgta
     6361 tgtccaatag aaacccctga aggtccgaac atcggtctga tcaactctct gtccgtgtac
     6421 gcacagacta acgaatacgg cttccttgag actccgtatc gtaaagtgac cgacggtgtt
     6481 gtaactgacg aaattcacta cctatctgct atcgaagaag gcaactacgt tatcgcccag
     6541 gcgaactcca acctggatga agaaggccac ttcgtagaag acctggtaac ctgccgtagc
     6601 aaaggcgaat ccagcttgtt cagccgcgac caggttgact acatggacgt atccacccag
     6661 caggtggtat ccgtcggtgc gtccctgatc ccgttcctgg aacacgatga cgccaaccgt
     6721 gcattgatgg gtgcgaacat gcaacgtcag gccgttccga ctctgcgcgc tgacaagccg
     6781 ctggttggta ctggtatgga acgtgctgtt gccgttgact ccggtgtaac tgcggtagct
     6841 aaacgtggtg gtgtcgttca gtacgtggat gcttcccgta tcgttatcaa agttaacgaa
     6901 gacgagatgt atccgggtga agcaggtatc gatatctaca acctgaccaa atacacccgt
     6961 tctaaccaga acacctgtat caaccagatg ccgtgtgtgt ctctgggtga accggttgaa
     7021 cgtggcgacg tgctggcaga cggtccgtcc accgacctcg gtgaactggc gcttggtcag
     7081 aacatgcgcg tagcgttcat gccgtggaat ggttacaact tcgaagactc catcctcgta
     7141 tccgagcgtg ttgttcagga agaccgtttc accaccatcc acattcagga actggcgtgt
     7201 gtgtcccgtg acaccaagct ggggccagaa gagatcaccg ctgacatccc gaacgtgggt
     7261 gaagctgcgc tctccaaact ggatgaatcc ggtatcgttt acattggtgc ggaagtgacc
     7321 ggtggcgaca ttctggttgg taaggttacg ccgaaaggtg aaactcagct gaccccagaa
     7381 gaaaaactgc tgcgtgcgat cttcggtgag aaagcgtctg acgttaaaga ctcttctctg
     7441 cgcgtaccaa acggtgtatc cggtacggtt atcgacgttc aggtctttac tcgcgatggc
     7501 gtagaaaaag acaaacgtgc gctggaaatc gaagaaatgc agctcaaaca ggcgaagaaa
     7561 gacctgtctg aagaactgca gatcctcgaa gcgggtctgt tcagccgtat ccgtgctgtg
     7621 ctggtagccg gtggcgttga agctgagaag ctcgacaaac tgccgcgcga tcgctggctg
     7681 gagctgggcc tgaccgacga agagaaacaa aatcagctgg aacagctggc tgagcagtat
     7741 gacgaactga aacacgagtt cgagaagaaa ctcgaagcga aacgccgcaa aatcacccag
     7801 ggcgacgatc tggcaccggg cgtgctgaag attgttaagg tatatctggc ggttaaacgc
     7861 cgtatccagc ctggtgacaa gatggcaggt cgtcatggta acaagggtgt aatttctaag
     7921 atcaacccga tcgaagatat gccttacgat gaaaacggta cgccggtaga catcgtactg
     7981 aacccgctgg gcgtaccgtc tcgtatgaac atcggtcaga tcctcgaaac ccacctgggt
     8041 atggctgcga aaggtatcgg cgacaagatc aacgccatgc tgaaacagca gcaagaagtc
     8101 gcgaaactgc gcgaattcat ccagcgtgcg tacgatctgg gcgctgacgt tcgtcagaaa
     8161 gttgacctga gtaccttcag cgatgaagaa gttatgcgtc tggctgaaaa cctgcgcaaa
     8221 ggtatgccaa tcgcaacgcc ggtgttcgac ggtgcgaaag aagcagaaat taaagagctg
     8281 ctgaaacttg gcgacctgcc gacttctggt cagatccgcc tgtacgacgg ccgcactggt
     8341 gaacagttcg agcgtccggt aaccgttggt tacatgtaca tgctgaaact gaaccacctg
     8401 gtcgacgaca agatgcacgc gcgttccacc ggttcttaca gcctggttac tcagcagccg
     8461 ctgggtggta aggcacagtt cggtggtcag cgtttcgggg agatggaagt gtgggcgctg
     8521 gaagcatacg gcgcagcata caccctgcag gaaatgctca ccgttaagtc tgatgacgtg
     8581 aacggtcgta ctaagatgta taaaaacatc gtggacggca accatcagat ggagccgggc
     8641 atgccagaat ccttcaacgt attgttgaaa gagattcgtt cgctgggtat caacatcgaa
     8701 ctggaagacg agtaattctc gctcaaacag gtcactgctg tcggggtaaa acccggcagc
     8761 ggattgtgct aactccgacg ggagcaaatc cgtgaaagat ttattaaagt ttctgaaagc
     8821 gcagactaaa accgaagagt ttgatgcgat caaaattgct ctggcttcgc cagacatgat
     8881 ccgttcatgg tctttcggtg aagttaaaaa gccggaaacc atcaactacc gtacgttcaa
     8941 accagaacgt gacggccttt tctgcgcccg tatctttggg ccggtaaaag attacgagtg
     9001 cctgtgcggt aagtacaagc gcctgaaaca ccgtggtgtc atctgtgaga agtgcggcgt
     9061 tgaagtgacc cagactaaag tacgccgtga gcgtatgggc cacatcgaac tggcttcccc
     9121 gactgcgcac atctggttcc tgaaatcgct gccgtcccgt atcggcctgc tgctcgatat
     9181 gccgctgcgc gatatcgaac gcgtactgta cttcgaatcc tatgtggtta ttgaaggcgg
     9241 tatgaccaac ctggaacgcc agcagatcct gactgaagag cagtatctgg acgcgctgga
     9301 agagttcggt gacgaattcg acgcgaaaat gggtgcggaa gcaatccagg ccctgctgaa
     9361 gagcatggat ctggagcaag agtgcgaaca gctgcgtgaa gagctgaacg aaaccaactc
     9421 cgaaaccaag cgtaaaaagc tgaccaagcg tatcaaactg ctggaagcgt tcgttcagtc
     9481 tggtaacaaa ccagagtgga tgatcctgac cgttctgccg gtactgccgc cagatctgcg
     9541 tccgctggtt ccgctggatg gtggtcgttt cgcgacttct gacctgaacg atctgtatcg
     9601 tcgcgtcatt aaccgtaaca accgtctgaa acgtctgctg gatctggctg cgccggacat
     9661 catcgtacgt aacgaaaaac gtatgctgca ggaagcggta gacgccctgc tggataacgg
     9721 tcgtcgcggt cgtgcgatca ccggttctaa caagcgtcct ctgaaatctt tggccgacat
     9781 gatcaaaggt aaacagggtc gtttccgtca gaacctgctc ggtaagcgtg ttgactactc
     9841 cggtcgttct gtaatcaccg taggtccata cctgcgtctg catcagtgcg gtctgccgaa
     9901 gaaaatggcg ctggagctgt tcaaaccgtt catctacggc aagctggaac tgcgtggtct
     9961 tgctaccacc attaaagctg cgaagaaaat ggttgagcgc gaagaagctg tcgtttggga
    10021 tatcctggac gaagttatcc gcgaacaccc ggtactgctg aaccgtgcac cgactctgca
    10081 ccgtctgggt atccaggcat ttgaaccggt actgatcgaa ggtaaagcta tccagctgca
    10141 cccgctggtt tgtgcggcat ataacgccga cttcgatggt gaccagatgg cagttcacgt
    10201 accgctgacg ctggaagccc agctggaagc gcgtgcgctg atgatgtcta ccaacaacat
    10261 cctgtccccg gcgaacggcg aaccaatcat cgttccgtct caggacgttg tactgggtct
    10321 gtactacatg acccgtgact gtgttaacgc caaaggcgaa ggcatggtgc tgactggccc
    10381 gaaagaagca gaacgtctgt atcgctctgg tctggcctct ctgcatgcgc gcgttaaagt
    10441 gcgtatcacc gagtatgaaa aagatgctaa cggtgaatta gtagcgaaaa ccagcctgaa
    10501 agacacgact gttggccgtg ccattctgtg gatgattgta ccgaaaggtc tgccttactc
    10561 catcgtcaac caggcgctgg gtaaaaaagc aatctccaaa atgctgaaca cctgctaccg
    10621 cattctcggt ctgaaaccga ccgttatttt tgcggaccag atcatgtaca ccggctttgc
    10681 atatgcagcg cgttctggtg catctgttgg tatcgatgac atggtcatcc cggagaagaa
    10741 acacgaaatc atctccgagg cagaagcaga agttgctgaa attcaggagc agttccagtc
    10801 tggtctggta actgcgggcg aacgctacaa caaagttatc gatatctggg ctgcggcgaa
    10861 cgatcgtgta tccaaagcga tgatggataa cctgcaaact gaaaccgtta ttaaccgtga
    10921 cggtcaggaa gagaagcagg tttccttcaa cagcatctac atgatggccg actccggtgc
    10981 gcgtggttct gcggcacaga ttcgtcagct tgctggtatg cgtggtctga tggcgaagcc
    11041 ggatggctcc atcatcgaaa cgccaatcac cgcgaacttc cgtgaaggtc tgaacgtact
    11101 ccagtacttc atctccaccc acggtgctcg taaaggtctg gcggataccg cactgaaaac
    11161 ggcgaactcc ggttacctga ctcgtcgtct ggttgacgtg gcgcaggacc tggtggttac
    11221 cgaagacgat tgtggtactc atgaaggtat catgatgact ccggttatcg aaggtggtga
    11281 cgttaaagag ccgctgcgcg atcgcgtact gggtcgtgta actgctgaag acgttctgaa
    11341 gccgggtact gctgatatcc tcgttccgcg caacacgctg ctgcacgaac agtggtgtga
    11401 cctgctggaa gagaactctg tcgacgcggt taaagtacgt tctgttgtat cttgtgacac
    11461 cgactttggt gtatgtgcgc actgctacgg tcgtgacctg gcgcgtggcc acatcatcaa
    11521 caaaggtgaa gcaatcggtg ttatcgcggc acagtccatc ggtgaaccgg gtacacagct
    11581 gaccatgcgt acgttccaca tcggtggtgc ggcatctcgt gcggctgctg aatccagcat
    11641 ccaggtgaaa aacaaaggta gcatcaagct cagcaacgtg aagtcggttg tgaactccag
    11701 cggtaaactg gttatcactt cccgtaacac cgaactgaaa ctgatcgacg aattcggtcg
    11761 taccaaagaa agctacaaag taccttacgg tgcggtactg gcaaaaggcg atggcgaaca
    11821 ggttgctggc ggcgaaaccg ttgcaaactg ggacccgcac accatgccgg ttatcaccga
    11881 agtaagcggt tttgtacgct ttactgacat gatcgacggt cagaccatta cgcgtcagac
    11941 cgacgaattg accggtctgt cttcgctggt ggttctggat tccgcagaac gtaccgcagg
    12001 tggtaaagat ctgcgtccgg cactgaaaat cgttgatgct cagggtaacg atgtactgat
    12061 cccgggtacc gatatgcctg cgcagtactt cctgccgggt aaagcgattg ttcagctgga
    12121 agatggcgta cagatcagct ctggtgacac cctggcgcgt attccgcagg aatctggcgg
    12181 taccaaggac atcaccggtg gtctgccgcg cgttgcggat ctgttcgaag cacgtcgtcc
    12241 gaaagagccg gcaatcctgg ctgaaatcag cggtatcgtt tccttcggta aagaaaccaa
    12301 aggtaaacgt cgtctggtta tcactccggt agacggtagc gatccgtacg aagagatgat
    12361 tccaaaatgg cgtcagctca atgtgttcga aggtgaacgt gtagaacgtg gtgacgtaat
    12421 ttccgatggt ccggaagcgc cgcacgacat tctgcgtctg cgtggtgttc atgctgttac
    12481 tcgttacatc gttaacgaag tacaagacgt ataccgtctg cagggcgtta agattaacga
    12541 taaacacatc gaagttatcg ttcgtcagat gctgcgtaaa gctaccatcg ttaacgcggg
    12601 cagctccgac ttcctggaag gtgaacaggt tgaatactct cgcgtcaaga tcgcaaaccg
    12661 cgaactggaa gcgaacggca aagtgggtgc aacttactcc cgcgatctgc tgggtatcac
    12721 caaagcgtct ctggcaaccg agtccttcat ctccgcggca tcgttccagg agaccactcg
    12781 cgtgctgacc gaagcagccg ttgcgggcaa acgcgacgaa ctgcgcggcc tgaaagagaa
    12841 cgttatcgtg ggtcgtctga tcccggcagg taccggttac gcgtaccacc aggatcgtat
    12901 gcgtcgccgt gctgcgggtg aagctccggc tgcaccgcag gtgactgcag aagacgcatc
    12961 tgccagcctg gcagaactgc tgaacgcagg tctgggcggt tctgataacg agtaatcgtt
    13021 aatccgcaaa taacgtaaaa actcgcttcg gcgggttttt ttatgggtgt tattcagagg
    13081 gcaattcttc acggaatttt taagaaaggc agggcgaaac gaggaagaag cttttcgccc
    13141 tggacaacat tcctgctgac gggactacca taaaaaaaca caacgctttc aacaaccttg
    13201 cgtttttcat cttccgtagc ggctcggaat aaatttacgt tgttacattc tgtctcatgg
    13261 cctttgcgag gcgcgtccca gatagctgtc ccagtctttc cataccggct gcaaaccctg
    13321 agcagttaac gcggcagcaa ccgcttccgg tctgcgatcg tcgtgcggtg agaactgttc
    13381 cagctcgggg tgattgtcgg catagccacc tggctgcgtt ttcgaaaaag cgctgacgtt
    13441 attaattgcc agcggaataa cgcgatcgcg aaaccacggt gattcccgcg tggagagtga
    13501 cagttcaatc tccggtgcaa gcagccggaa ggcgcagatg gtttgcacta actggcgttc
    13561 atccataatc gacgcaggct caatgccgcc agtacatgga cgcaggcgtg ggaaggagat
    13621 agagtagcgg ctttgccagt aatgctgttg cagccatagc aaatgttctg caaccatata
    13681 gcagtcaacg cgccagctgt cggaaaggcc aattagcgcg ccgaggccta tcttatcaat
    13741 ccccgcacgg cccagccgat ccggcgtttc cagccgccag aagaagtcct gttttttacc
    13801 tttcagatga tggcgggcat aagtcgcctc gtgatatgtc tcctgataaa ccatcacgcc
    13861 atccagacct agttgcttta actcggcgta ttccgtctcc gccagcggtt gcacttccat
    13921 ctgtagtgaa gagaactgtt cacgcagggc agggagatga cgacgaaagt aatccatccc
    13981 cactttcacc tgatgttcac cagtgactaa tagcagatgt tcaaagccca tctcccgtat
    14041 agcggcgctt tccctggcaa tatccgcttc atccagcgtt ttgcgcttga tgcgattgct
    14101 catggaaaat ccgcagtacg tgcagtcgtt agcgcaaaga ttggaaagat aaagcgggac
    14161 gtagaagctg acgacgttgc caaatcgctg acgggtcaga cgctgcgccc gttgggccag
    14221 tggttccaga tagccactgg cggctggcga taacagcgcc atcatatcgt cgcgggtgag
    14281 ttgcgaggca tttagcgccc gctctacgtc agcagccgtt ttgctgttga tacgcaggcg
    14341 gatgtcgtcc cagtccagtt gtcgccagcg atcgctgaag gttttcatgc tgatgcctcc
    14401 agaaatccgg tcagcgggct ggtggcatga gcaaaatgac tgcggctgcc cggtccggac
    14461 tgacgtgcca gcaggcctgc ttctactgcc agacgaaatg ccttcgccat gttgacggga
    14521 tcgtccgcga cggcaatcgc cgtattcact aacaccgcgt cggcccccat ttccagcgcc
    14581 tgcgcggcat ggctgggaac gccgatgcca gcatcgacaa ccaccggaac cgtggcttgc
    14641 tgggtaataa tctccagcat ggcacgggtt tccagtccct gattcgagcc aatcggcgcg
    14701 ccgagcggca tcaccgctgc acagccgact tcttccagac gtttacacaa taccggatcg
    14761 gccccgcagt aaggcagcac gacaaatccc tgttgtacca gcgtttcggc ggctttcagg
    14821 gtttcgatgg gatcgggcaa cagccagcgg gcgtcagggt gaatctctaa ttttaaccag
    14881 tttgtgccta acgcttcacg agccagatgg gcggcgaaaa tggcttcttc cgctgttttc
    14941 gccccggatg tatttggcag cagggtcaca cccgccgcga taagcggttc gaggatagcg
    15001 tcgttgtgct ggcgcaagtt gacgcgtttc atcgccagtg tcaccagttg gctgccgcaa
    15061 gcgcggatcg cctccatcat cagttgtgaa gaggcgaatt tccctgtgcc ggtaaacaga
    15121 tgtgaatcaa acgttttgtc cgcaatacgt aacatctcaa ccccctgcaa taacctgaaa
    15181 aagcaggatc tggtcgccat cctgcacgat atgttgcgcc cactgctcac gcgggacgat
    15241 ttgctgatta atcgccagag ccgcgcccga ttgtcgttgg tcgagttgct ccagtagttc
    15301 gtgaaccgtt tgcccggcgg cgcactgcat cggttgatcg ttaaacagga tctgcattgc
    15361 ttcctccgca taccgggcaa ccactggcgc ggcgcaacgc caggttgcgc cactggctcg
    15421 atttaccgtc gaacagtcgg agttctcccg caggcgtctc tataccgctt aataacttaa
    15481 tggcttccag tgcctgtaaa gtgcccataa ccccgaccac cgggccaacc acgcccgccg
    15541 tgcggcagtt gcgttcaggc tcctggttat ctggccacag gcagcggtaa cacccctgct
    15601 cccagggcgg cgtcagtacc atcaactgac cgccaaatcc gaccgcgctg gcggtgataa
    15661 gcggcgtgtt gagtgccacg caggcggcat taatctcctg acgagtcgcc atattgtcgg
    15721 tacagtcgag caccacatcg gcccgtgcta ccgcatcttt taacgcctca cccgttaacc
    15781 gttgttgtaa tgctgtcagt tgaatatcgg gattcaactg tgtcagtcgc tgatggctga
    15841 cctgcgattt cgggcgatcg atatcttcag tggtaaagag gatttgtcgt tgcagattgc
    15901 ttaaatgcac atcgtcgtca tctgccagta ccagcgtccc gacgccagcg cccgccaggt
    15961 atagcgcagc aggtgtaccc agcccgccaa gaccgataat cagcacctgg ctgtcgagca
    16021 gtttttgctg cccgtcaaga gcgatatcgt cgagcaggat ttggcggcta taacgcataa
    16081 agtcacggtc attcatcgcc aactcctgca atttccagca actgtgccgt tgccaaacgc
    16141 cagtctgcgg cttgagtaat ggcgcttacg acggcgatgc tgccgacacc cgttgctatc
    16201 accgcaggcg cgcgcgccag actgataccg ccaatcgcca cggtgggata atccgccagg
    16261 cgctcaacat gccgtgccag ctgttccagc ccctgcggtg cagaaggcat ctgtttggtt
    16321 tgcgtcggga acacatgtcc cagcgcgata taagaggggc gtgctgccag cgcgacgtcg
    16381 atctccatat cgtcatgggt cgaaacgccc agtcgcagac ctgccgcgcg gatggcgctg
    16441 agatcggtgg cttgcaaatc ttcctgcccc agatggacgc catatgcctg atgcttgatc
    16501 gccagccgcc agtaatcgtt gataaacaat cgcgcgttat agcggcggcc cagcgcaatt
    16561 gccgccacga catcggcttc cacctcttca tcgcgtcgat ctttgatgcg tagctggaga
    16621 gtacgtacgc ctgcatccaa cagacgttcg atccactgta cgctgtccac caccgggtac
    16681 agtcctaaac gaaagggtac aggaggaaaa tctggctgat acatcacgct tcctccttac
    16741 gcaggtagat ttctccgcct ctggcgcgga agttctccga catatcagcc attcccactt
    16801 caatggtttg cgcggcggcg taatcgcgca cttcctggct gattttcatc gagcagaatt
    16861 tcggcccaca catggagcaa aaatgggcga ctttgccgga ttcttgcggc agggtttcat
    16921 cgtgataagc gcgggcggta aacgggtcga gggccagatt aaactggtct tcccagcgaa
    16981 attcgaagcg ggctttcgac atggcgttat cgcgaatttg cgcgcccgga tgccctttcg
    17041 ccaggtcagc ggcgtgggcg gcaatcttat aggtgataag cccctgctta acatcttctt
    17101 tattaggcag acccagatgc tcttttggcg ttacgtaaca gagcatcgcg cagccaaacc
    17161 agccaatcat cgccgcacca atccccgacg tgaagtggtc ataacccggc gcgatatcgg
    17221 tagttagcgg ccccagagtg taaaacggcg cttcgtggca gtgctctaac tcctcggtca
    17281 tattgcggcg gatcatctgc atcggcacgt ggcctgggcc ttcaatcatc acctgtacgt
    17341 catattccca ggcgattttg gtcagttcac ccagcgtatg cagctcggca aattgcgctt
    17401 catcgttggc gtcctgaata gaaccagggc gcagaccgtc tcccagcgac agcgaaacgt
    17461 cataagcggc acagatttca caaatttcgc ggaagtgttg atagaggaaa ttttcctgat
    17521 gatgggagag gcaccatttc gccataatcg aaccgccgcg agagacgata ccggtcaggc
    17581 gtttcgcggt catcggcaca tagcgcagca gtacgcccgc atggatagtg aagtaatcca
    17641 caccttgctc ggcctgttcc agcagcgtgt cgcggaacgc ttcccaggta agatcttcgg
    17701 cgatcccgtt aaccttctcc agcgcctggt agatcggcac tgtaccgatc ggcaccgggc
    17761 tgttacgcaa aatccactcg cgggtttcgt gaatatagcg accggtggag agatccatca
    17821 ccgtatccgc tccccagcgc gtggaccata ccagcttttc cacttcttct tcgatggaag
    17881 aggtgaccgc cgagttgccg atattggcgt taacttttac caggaaattg cgaccaataa
    17941 tcatcggctc cgattccgga tgattaatgt tggccgggat aatcgcgcgt ccggcagcaa
    18001 cttcatcacg gacaaattcc gcagtgatat tttccggcag acgtgcgcca aagctcattc
    18061 ccggatgctg gtggcgtaaa acttcgctac gtatgtgctc gcggcccata ttctcgcgga
    18121 tggcgatgaa ttccatttcc ggtgtgatga tgccctggcg ggcgtagtgc agttgggtga
    18181 cgcggcgtcc tgctttggcg cgttttggtg ttagtacgcc gctaaaacgc agctcgtcga
    18241 ggccatcatc tgccagccgc gctttagtgt aatcggaact gcgcacggta agttcttcgg
    18301 tatcgccgcg cgcatcgatc cacggctggc gtagttttgc cagtccttgc tgcacgttaa
    18361 tggcgatctg tggatcgcca tacggaccgg aggtgtcgta gaccggaatc gcttcgtttt
    18421 cttcgtactg cggttgttct ttgctgccgc caattagcgt cgggctaagc tggatctcac
    18481 gcatcggcac gcgcacgccg gggtgtgtgc cagtgatgta aatgcgtttt gagttgggaa
    18541 aggcggtgcc ttccagggtg tcgataaaat gttgggcctg ggcgcgttgt tcgcggcggg
    18601 tcagttttgt tacagacata gctcattcca aaaagttaag gacgtggctt gtcagacgac
    18661 ggatgaagca agagacgatc gccgcaaagg cgatgcgata gcagattaac tcttgttccc
    18721 ttcgcaggta ttagcctgat caggttccgc ggatcccgaa taaacggtct cagccagtta
    18781 aggcactccg acaagaaatt agccccacaa acggggcgtt gaatgtaaat tacgcgttaa
    18841 cagcgcagaa ctcaagcagg atgtttgacg cgggcggcgt caagtaccag cagaatcaac
    18901 ttatcttcca gcacaaagcg tgcttccagc gcctcgccga tgtcagataa aacctgttga
    18961 aactcaaggt aattatcatg atcgatggcg gtttccaggc tggaatcgta gtaatccata
    19021 atctgttggg tattggcttc gagttgcggc caaatcttcg cggcgcgcgc gagttgcccg
    19081 ttgccttcca gcttatgaag aatgcgctca taaatactga aatgtccggc agacaagtaa
    19141 tcgaccaggc tctgacaaaa atcatcaagg gctttttcgt ttagcctcat gtacgattct
    19201 ttgccaggct taatgccaac cagattgtag taagccacga gcagatgctt acgtacatgt
    19261 agccagcgat caaccagttt gttacttcct ctgacgcgtt ccgtcaggtt atcgagctgg
    19321 ttaagcatga ttgactccgc aagtttgtat tcaaaaactg ctcagtgaga aatgtaaaaa
    19381 ccatgttaaa catgccagtg atgcaaaggt agtgcaagag ctatggatcg tataattgaa
    19441 aaattagatc acggctggtg ggtcgtcagc catgaacaaa aattatggtt gccgaaggga
    19501 gaattgccat atggcgaagc ggcaaatttc gatcttgtgg gtcagcgcgc actacaaatc
    19561 ggcgaatggc aaggggaacc tgtttggtta gtacaacagc agcggcgtca cgatatgggg
    19621 tcggtacgtc aggtcattga tctcgatgta gggctgtttc aactggccgg acgtggcgtg
    19681 caactggcgg agttttaccg atcgcataaa tactgtggtt actgcgggca tgaaatgtat
    19741 ccaagcaaaa ccgaatgggc gatgctgtgc agccattgcc gtgagcgtta ctacccgcaa
    19801 atcgccccct gcattattgt tgccatccgt cgcgatgatt cgatcctcct cgcccagcat
    19861 acccgccatc gtaacggtgt ccatacagta cttgccggat tcgtcgaagt gggtgaaacc
    19921 ctcgagcagg cagtcgcgcg ggaagtgatg gaagagagcg gaattaaagt taaaaacttg
    19981 cgttacgtga cctcccaacc gtggccgttt cctcagtcgt taatgaccgc gtttatggcg
    20041 gaatatgaca gcggcgacat cgttatcgac ccgaaagaat tgctcgaggc gaactggtat
    20101 cgctatgacg atttgccgtt actcccgccg cccggcaccg tagcgcgccg tctgatagaa
    20161 gatacggtgg cgatgtgtcg ggcagagtat gagtgatgat acactgaccg cctgacgcac
    20221 taaggaacag ccaaaatgac cgaacttaaa aacgatcgtt atctgcgggc gctgctgcgc
    20281 cagcccgttg atgtcactcc agtatggatg atgcgccagg cgggtcgcta tctaccggaa
    20341 tataaagcca cgcgcgccca ggcgggcgat tttatgtcgc tgtgcaaaaa tgccgagctg
    20401 gcgtgcgaag tgactttgca accgctgcgt cgctatccgc tggatgcggc gatcctcttt
    20461 tccgatatcc tcaccgtgcc agacgcgatg ggtttagggc tctattttga agccggagaa
    20521 ggtccgcgtt ttacctcacc agtcacctgc aaggccgacg tcgataaact gccaattccg
    20581 gacccggaag atgagctggg ttacgtgatg aacgcggtgc gtaccattcg tcgcgaactg
    20641 aaaggcgaag tgccgctgat tggtttttcc ggcagcccgt ggacgctggc gacctatatg
    20701 gtggaaggcg gcagcagcaa agcgttcacc gtgatcaaaa aaatgatgta tgccgatcca
    20761 caggcgctgc acgcgctact cgataaactg gcgaaaagcg tcactttgta tctgaatgcg
    20821 cagattaaag ccggtgctca ggctgtgatg attttcgaca cctggggcgg cgtgcttacc
    20881 gggcgcgatt atcaacagtt ctcgctctat tacatgcata aaattgttga tggtttactg
    20941 cgtgaaaacg acggtcgccg cgtaccggtc acgctgttta ccaaaggcgg cggacagtgg
    21001 ctggaagcca tggcagaaac cggttgcgat gcgctgggcc tcgactggac aacggacatc
    21061 gccgatgcac gccgccgcgt gggcaataaa gtcgcgttgc agggcaatat ggatccgtcg
    21121 atgctgtacg cgccgcctgc ccgcattgaa gaagaagtag cgactatact tgcaggtttc
    21181 ggtcacggtg aaggtcatgt ctttaacctt ggtcacggca ttcatcagga tgtgccgcca
    21241 gaacatgctg gcgtgtttgt ggaggcagtg catcgcctgt ctgcgcctta tcatctgtaa
    21301 ggagtggtta tggatctcgc gtcattacgc gctcaacaaa tcgaactggc ttcttctgtg
    21361 atccgcgagg atcgactcga taaagatcca ccggatctga tcgccggagc cgatgtcgga
    21421 tttgagcagg gcggagaagt gacgcgagcg gcgatggtgc tgctgaaata cccctcgctt
    21481 gagctggtgg agtataaagt ggcccgcatc gccaccacca tgccttacat tcctggtttt
    21541 ctctcctttc gtgaatatcc tgcgctgctg gcagcgtggg agatgctgtc acaaaagccg
    21601 gatttagtgt ttgtcgatgg tcatgggatc tcgcatcctc gccgtcttgg cgtcgccagc
    21661 cattttggtt tgctggtgga tgtgccgacc attggcgtag cgaaaaaacg gctctgcggt
    21721 aaattcgaac cgctctccag cgaaccgggc gcgctggccc cgctgatgga taaaggcgag
    21781 cagctggcct gggtctggcg cagcaaagcg cgctgtaacc cattgtttat cgctaccggc
    21841 catcgggtca gcgtggacag cgcgctggcg tgggtacaac gctgcatgaa aggctatcgt
    21901 ctgccggagc caacgcgctg ggcggacgcg gtggcctcgg aacgtccggc gttcgtgcgc
    21961 tatacagcaa atcagcccta attcaggtaa actgcggata atttccgtat ttgagaactc
    22021 aacatgttac aaaacccaat tcatctgcgt ctggagcgcc tggaaagctg gcagcacgtc
    22081 acttttatgg cctgcttatg cgaacgtatg taccccaatt acgccgtatt ctgtcagcaa
    22141 accggcttcg gcgatgggca gatttaccgc cgcattctcg atctcatttg ggaaacgctg
    22201 accgtcaaag acgcaaaagt aaatttcgac agccaactgg agaaatttga agaagcgatt
    22261 ccttcagccg acgatttcga tctgtacggc gtttatccgg caatcgatgc ttgcgtggcg
    22321 ttaagtgaac tggtccattc gcgtttaagt ggcgaaacgc tcgaacacgc ggtggaagtg
    22381 agtaagactt ccatcacgac cgtggcgatg ctggaaatga ctcaggctgg tcgcgaaatg
    22441 agcgatgaag agctcaaaga aaacccagct gtagagcaag aatgggacat tcagtgggaa
    22501 atattccgac ttttagctga gtgcgaagaa cgcgatatcg agctgataaa aggccttagg
    22561 gcagacctgc gtgaggcggg tgagagcaat attggtataa tttttcagca ataagaccag
    22621 aaaacgtgat ttaacgcctg atttgtcgta cctggagtct tccctttcgc cccccgtctg
    22681 gtctacattt ggggggcgaa aaaaagtggc tatcggtgcg tgtatgcagg agagtgctat
    22741 tctggcattt ccgtcgcact cgatgcttag caagcgataa acacattgta aggataactt
    22801 atgaacaaga ctcaactgat tgatgtaatt gcagagaaag cagaactgtc caaaacccag
    22861 gctaaagctg ctctggagtc cactctggct gcaattactg agtctctgaa agaaggcgat
    22921 gctgtacaac tggttggttt cggtaccttc aaagtgaacc accgcgctga gcgtactggc
    22981 cgcaacccgc agaccggtaa agaaatcaaa attgccgcag ctaacgtacc ggcatttgtt
    23041 tctggcaagg cactgaaaga cgcagttaag taagattgcg tggcagtgaa cagttttaac
    23101 gaaggggtgg tttcacccct tttgtctttc tggcgtcgat cattgatgct ggctggcgct
    23161 ctgtttctca ctgcttgtag tcacaactct tcacttcctc cctttaccgc cagtggattt
    23221 gctgaagacc agggcgcggt acgcatctgg cgaaaagaca gcggcgataa tgtgcatctt
    23281 cttgccgtgt ttagcccgtg gcgcagtggc gatacgacga cgcgagagta tcgctggcag
    23341 ggcgataacc tcacgctcat caatatcaat gtttacagca aacccccggt gaatattcgc
    23401 gcgcgttttg acgatcgcgg cgatctgagc tttatgcaac gcgaatccga tggggaaaag
    23461 cagcagcttt ctaacgacca aatcgattta taccgttatc gtgctgatca gatccgccag
    23521 attagcgatg ccttacgtca ggggagagtc gtgctgcgcc aggggcgctg gcatgcgatg
    23581 gagcagactg tgaccacctg cgaagggcaa accattaaac ctgatttaga ttcgcaggcg
    23641 atagcgcata tcgagcgccg ccagagccgc tcttctgttg atgtcagcgt ggcatggctg
    23701 gaagcgcccg aaggttcgca attactgttg gtggcaaact ctgatttctg tcgctggcaa
    23761 cccaacgaga aaacgttctg atttaccagt ggtccatacc catatgaccg ccaccaccgc
    23821 agccgccgta acccattccg gtaccgcgcg gaatacccgc ttcagccatc gcaatatctc
    23881 gtttcacccg taactcatct aacgactgac gcaaattctc catctctttg gcgaccgcgt
    23941 taattttgct gctatccggt gggttcgcgg ctaacagggc attgtattca taacgctttg
    24001 ttaccagttg ctgttgcagt gcgctgcttt gagcgtaaaa gtcattatgg attttctgcc
    24061 acgctgtctg ttgttcgctg gtcaaaggcg cggcattttg ctgccacata ccgtgtccgc
    24121 cgtgagcaaa tgccgatgtc gatcccatcg ccattgctga aagcgccatc attaccaggg
    24181 caattttcgt gttccgtttc atggttaatc ctccagtggt tgtcttactt cgggtattgc
    24241 atcttccgtg ccaacgatga aacgctgata tgacgggtaa tctggcatga taaacgagta
    24301 aaaatgactc gcctgctgcg ggtagcgagt catttttact cattgaaact taagcctttg
    24361 tgttacagcg cagggtaagc gctgataaaa gatggcatga tttctgctgt cagaaaggga
    24421 tgagcaggca aagaagaaga tgcgttttat gcaacgttct aaagactcct tagctaaatg
    24481 gttaagcgcg atcctccccg tggtcattgt tgggctggtg gggctgtttg cggtgacagt
    24541 gattcgcgat tacgggcgcg agactgcagc cgccagacaa acgctgctgg aaaaaggcag
    24601 tgtacttatc cgtgctctcg aatccggctc gcgcgtcggc atggggatgc gcatgcatca
    24661 tgcgcagcag caggcattac tggaagaaat ggccgggcag cctggtgtac gttggtttgc
    24721 ggtcacggat gaacaaggaa caatcgtgat gcatagcaac tccggcatgg tgggaaaaca
    24781 gctttattcc ccgcaggaaa tgcagcagtt acatccggga gatgaagaag cgtggcggcg
    24841 gatcgatagc gcagacggcg agcctgttct ggaaatttat cgccagtttc aaccgatgtt
    24901 tgctgctgga atgcaccgga tgcgccatat gcagcagtat gccgcgacac cacaagcaat
    24961 ttttatcgct ttcgacgcca gtaacattgt gagtgccgaa gatcgtgagc agagaaacac
    25021 cctgattatc ctcttcgccc tggcgacggt cttgctggca agcgtgttgt cattcttctg
    25081 gtatcgccga tatctgcgct cgggccagct gttgcaggat gaaatgaagc gcaaagagaa
    25141 gctggtggca ctggggcatc ttgcggcagg cgttgcccac gaaatccgta acccactttc
    25201 ctcgattaaa gggctggcga aatactttgc cgaacgcgcg ccagcagggg gagaagcgca
    25261 tcaactggcg caggtgatgg cgaaagaagc cgaccgttta aaccgcgtgg taagcgagtt
    25321 gctggaactg gttaagccaa cgcatctggc tttgcaggcg gtggatctca acacgctgat
    25381 taaccactca ttacagctgg taagccagga tgcaaacagc cgggagatcc agttacgctt
    25441 taccgccaac gacacattac cggaaattca ggccgatccg gacaggctga ctcaggtcct
    25501 gttgaatctc tatctcaatg ctattcaggc gattggtcag catggcgtga ttagcgtgac
    25561 ggccagcgaa agcggcacgg gtgtgaaaat cagcgttacc gacagcggta agggaattgc
    25621 ggcggatcag cttgaagcca tcttcacccc gtatttcacc acgaaggccg aaggcaccgg
    25681 actggggctg gcggtcgtgc ataatattgt tgaacaacac ggtggtacaa ttcaggtcgc
    25741 aagccaggtg ggaaaaggcg caacgttcac cctctggctt ccggtcacta ttacgcgtaa
    25801 ggacccacaa ggatgacgca cgataatatc gatattctgg tggtggatga tgacattagc
    25861 cactgcacta ttttgcaggc tttgctgcgc ggctggggct ataacgtcgc gctggcgaac
    25921 agcgggcgac aggcgctgga gcaggtgcgg gaacaggttt ttgatcttgt gctttgcgat
    25981 gtgcgaatgg cggagatgga cggcattgcc acgctaaaag agatcaaagc gctaaatccg
    26041 gcgattccgg tactgattat gaccgcatac tccagcgtcg agacggcggt agaggcactg
    26101 aaaaccgggg cgctggatta tctcatcaag ccgctggatt tcgataacct gcaggcgacg
    26161 ctggaaaagg cgctcgcaca tacgcacagt gttgacgctg aaacgcctgc ggtgtctgcc
    26221 agccagttcg gtatggtagg taaaagcccg gcgatgcaac acctgctcag tgaaatcgcc
    26281 ctcgtcgcgc catcggaagc cacagtgctg atccacggcg attccggcac cggtaaagag
    26341 ctggtcgcaa gggcgattca cgccagtagc gcacggagtg aaaaaccact ggtaacgctc
    26401 aactgtgcgg cactcaacga atccttgctg gaatctgaat tgttcggtca cgaaaaaggg
    26461 gcgtttaccg gagccgataa acgccgggaa gggcgctttg ttgaggcgga cggcggcacg
    26521 ctgtttctcg atgaaattgg cgatatctcg ccgatgatgc aggttcgtct gctgcgtgcg
    26581 attcaggagc gcgaagttca gcgtgtcggc agcaaccaga ccatctcggt tgatgtccgg
    26641 ctgattgcgg cgactcatcg cgatcttgcc gcagaggtga atgccgggcg ttttcgccag
    26701 gatctctact atcgcctgaa cgtggtggcg attgaagttc cgtcgctgcg tcagcggcgg
    26761 gaagatattc ctctgctggc aaaccatttt cttcagcgct ttgccgagcg taatcgtaag
    26821 gcggtaaaag gttttacgcc ccaggcgatg gatctgctga ttcactacga ctggccggga
    26881 aatattcgtg aactggaaaa cacggtggaa cgggcggtgg tgctgctgac cggggaatat
    26941 atttccgaac gcgagctgcc gctggcgatt gcgagtacgc cgatcccgct ggtacaaagt
    27001 caggatattc agccgctggt ggaagtcgaa aaagaggtga ttctggcggc gctggagaaa
    27061 acgggcggca acaaaaccga agccgcccgc cagttaggga tcacgcgcaa aacgctattg
    27121 gcaaaactgt cgcgttagtt ctgctcgcgt tcgatagcgc gccagccgat atctttccgg
    27181 cagaagcagt cgtcccaatg gatatcggtc attaaggcat aagcgcgttt ctgcgcttct
    27241 gccacggtat gacccagcgc ggtaacgcac agtacgcgcc cgccgctggt cactacctgc
    27301 tcgtcatccg ctagtttcgt gcccgcgtgg aacactttgc cgtctgccac ttcttccagc
    27361 ggcaggccgt ggatcacatc gccggtgcgg taatcgcccg gatatccacc cgcagccatc
    27421 accacgccga gagaagcgcg ttcatcccac tcggacgttt tctcgtccag cttgccttca
    27481 caggccgcca ggcagagctc aaccagatcg gacttcatgc gcagcataat cggctgggtt
    27541 tctggatcgc caaagcggca gttaaattca ataaccttcg gattgccctg tttgtcgatc
    27601 atcaggcccg cgtagagaaa accagtgtag gtgttgcctt ctgccgccat gcctttcacg
    27661 gttggccaga tgattcgttc catcgtgcgc tgatgaactt catcggttac taccggcgcg
    27721 ggggagtaag cgcccatccc gcccgtgttt ggtccggtat ctttatcgcc tacgcgtttg
    27781 tgatcctggc tggtagccat cggcagcaca tgctcgccgt ccaccatcac gataaagctc
    27841 gcttcttcgc catcgaggaa ctcttcgata acgatgcgat gacccgcgtc gccaaacgcg
    27901 ttgcccgcca gcatatcgtg aacagctgct tcagcttctt ccagcgtcat cgccacgata
    27961 acgcctttcc cggctgccag accgtcagct ttaatgacga ttggcgcgcc tttctcacgc
    28021 agatacgcca gcgcaggttc tacctcggtg aagttctggt attccgctgt gggaatgtta
    28081 tggcgggcca gaaagtcttt ggtgaatgct ttagaacctt ccagttgtgc ggcacctgcg
    28141 gttgggccga agattttcag cccggcggcg cggaaggtat cgactacgcc tttcaccagc
    28201 ggcgcttccg ggccgacgat ggttagatcg atcttttcgt tttgcgcgaa atccagcagc
    28261 gccgggatat cggttacgcc gatagcgacg ttttgcagtg tgggttccag cgcagtgcct
    28321 gcattacccg gagcaacaaa aacagtttca accagcggcg actgggccgc tttccaggcc
    28381 agcgcgtgct cgcgcccgcc gttaccaatc actaatactt tcatctgtcg ctccattaat
    28441 ggcggaagtg gcgcatgtcg gtgaagagca tcgcaatacc gtgctcgtcg gcggcggcaa
    28501 tcacttcgtc atcacggata gaaccgccag gctggattac gcaggtcaca cccgcagcgg
    28561 cggcggcatc aataccgtcg cggaacggga agaacgcgtc agaagccatc gaggaacctt
    28621 tcacttccag gccttcatcg gccgctttaa taccggcgat ttttgcggag tacacgcggc
    28681 tcatctggcc cgcgccaatg ccgatggtca tattgttttt ggcatagaca atggcgttgg
    28741 atttcacgaa cttcgccact ttccagcaga acagcgcatc acgcagttcc tgttcggtcg
    28801 gctgacgttt ggtgacgacg cgcagttctt ctgcaccgac catgcccagg tcgcgatcct
    28861 gaaccagcag accgccgtta acacgtttga aatcaagacc cggaacacgc tcgccccact
    28921 gaccgcaggt caggacgcgt acgttctgtt tggcggcggt gattttcagg gcttcttcgc
    28981 tggcggacgg cgcaataatc acttcaacaa actgacgaga aatgatggcc tgcgcggttt
    29041 ccgcatccag ctcgcggtta aaggcgataa tgccgccgaa tgcggaggtc gggtcggttt
    29101 tgtacgcgcg atcgtaagca tcaagaatgg aattgctgat agccacgccg cacgggttgg
    29161 cgtgcttcac aatcacacat gctggctcgg cgaactcttt cacgcactcc agcgccgcat
    29221 cggtatcggc gatgttgtta taagagaggg ccttaccctg aacctgggtt gcggtggcaa
    29281 cagaggcttc tttcacgttc tcttctatat agaaggcagc ctgctggtgg ctgttctcgc
    29341 cgtaacgcat atcctgcttc ttaatgaagt tcaggttcag cgtgcgtggg aagcgaccgg
    29401 cggcttcttt gctttcaccg tggtaagccg gaaccatgct gccgaagtag ttggcaatca
    29461 tgctgtcgta ggcggcggta tgttcaaagg ctttgatagc gagatcgaaa cgggttgcaa
    29521 gcagaagcga tccttcgttg gcatccatct ctttaatgat ggcgtcgtag tcgctgctct
    29581 tcaccacgat ggcgacatct ttatggttct tggcggcaga gcgcaccatc gtcgggccgc
    29641 cgatatcgat gttctcaacc gcatcttcca gcgagcaacc ttcacgggca acggtctggg
    29701 cgaacggata caggttaaca accaccatat cgataggctg gatctgatgt tcttccataa
    29761 tggcatcgtc ctggccgcga cggcccagaa tgccaccatg tactttcgga tgcagggtct
    29821 tcacgcgtcc atccatcatc tccgggaaac cggtgtaatc ggaaacttcg gttaccggca
    29881 gacctttttc tgctaacaga cgggctgtgc cccctgtaga cagcagctcc acaccgcgtg
    29941 cggaaagtgc ctgggcgaat tcgacgatac cggctttgtc agaaacactg agcagagcgc
    30001 ggcggactgg acgacgttgt tgcatggtaa atcccctgga tttgactatt acagagagcg
    30061 ttagctgaat ttttcgcgaa aaactcagct aacgccccta acggggcatc cttatttttc
    30121 gcccgcattg taacgaaaac gtttgcgcaa cgctcgcgaa tttttctctt tcaatggtga
    30181 tcacaatttt gacagtggct accgtgggaa aaatacagaa attacattga tgattgtgga
    30241 taactctgtg cgtaaaaagg tataaagcgg ggttttgctg gggaatgcag cagtcagtca
    30301 tttttatgca atttttctat tgcgacctgc ggagaactcc ctataatgcg cctccatcga
    30361 cacggcggat gtgaatcact tcacacaaac agccggttcg gttgaagaga aaaatcctga
    30421 aattcagggt tgactctgaa agaggaaagc gtaatatacg ccacctcgcg acagtgagct
    30481 gaaagccgcg tcgca
//
LOCUS       DAZGQL010000065        30488 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.065, whole genome shotgun sequence.
ACCESSION   DAZGQL010000065 DAZGQL010000000
VERSION     DAZGQL010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30488
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.065"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            381..923
                     /gene="ecpR"
                     /locus_tag="ACHKIR_003167"
                     /gene_synonym="matA"
     CDS             381..923
                     /gene="ecpR"
                     /locus_tag="ACHKIR_003167"
                     /gene_synonym="matA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECP biosynthesis operon DNA-binding
                     transcriptional regulator EcpR"
                     /protein_id="HFO9990862.1"
                     /translation="MECQNRSDKYIWSPHDAYFYKGLSELIVDIDRLIYLSLEKIRKD
                     FVFINLNTDSLTEFINRDNEWLSAVKGKQVVLIAARKSEALANYWYYNSNIRGVVYAG
                     LSRDIRKELAYVINGRFLRKDIKKDKITDREMEIIRMTAQGMLPKSIARIENCSVKTV
                     YTHRRNAEAKLYSKLYKLVQ"
     gene            997..1584
                     /gene="ecpA"
                     /locus_tag="ACHKIR_003168"
                     /gene_synonym="matB"
     CDS             997..1584
                     /gene="ecpA"
                     /locus_tag="ACHKIR_003168"
                     /gene_synonym="matB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="common pilus major fimbrillin subunit EcpA"
                     /protein_id="HFO9990863.1"
                     /translation="MKKKVLAIALVTVFTGTGVAQAADVTAQAVATWSATAKKDTTSK
                     LVVTPLGSLAFQYAEGIKGFNSQKGLFDVAIEGDSTATAFKLTSRLITNTLTQLDTSG
                     STLNVGVDYNGAAVEKTGDTVMIDTANGVLGGNLSPLANGYNASNRTTAQDGFTFSII
                     SGTTNGTTAVTDYSTLPEGIWSGDVSVQFDATWTS"
     gene            1641..2309
                     /gene="ecpB"
                     /locus_tag="ACHKIR_003169"
                     /gene_synonym="yagY"
     CDS             1641..2309
                     /gene="ecpB"
                     /locus_tag="ACHKIR_003169"
                     /gene_synonym="yagY"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpB"
                     /protein_id="HFO9990864.1"
                     /translation="MKKHLLPLALLFSGISPAQALDVGDISSFMNSDSSTLSKTIQNS
                     TDSGRLINIRLERLSSPLDDGQVIAMDKPDELLLTPASLLLPAQASEVIRFFYKGPAD
                     EKERYYRIVWFDQALSDAQRDNANRSAVATASARIGTILVVAPRQANYHFQYANGSLT
                     NTGNATLRILAYGPCLKAANGKECKENYYLMPGKSRRFTRVDTADNKGRVALWQGDKF
                     IPVK"
     gene            2335..4860
                     /gene="ecpC"
                     /locus_tag="ACHKIR_003170"
                     /gene_synonym="yagX"
     CDS             2335..4860
                     /gene="ecpC"
                     /locus_tag="ACHKIR_003170"
                     /gene_synonym="yagX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher EcpC"
                     /protein_id="HFO9990865.1"
                     /translation="MPLRRFSPGLKAQFAFGMVFLFVQPDASAADISAQQIGGVIIPQ
                     AFSQALQDGMSVPLYIHLAGSQGRQDDQRIGSAFIWLDDGQLRIRKIQLEESEDNASV
                     SEQTRQQLMTLANAPFNEALTIPLTDNAQLDLSLRQLLLQLVVKREALGTVLRSRSED
                     IGQSSVNTLSSNLSYNFGVYNNQLRNGGSNTSSYLSLNNVTALREHHVVLDGSLYGIG
                     SGQQDSELYKAMYERDFAGHRFAGGMLDTWNLQSLGPMTAISAGKIYGLSWGNQASST
                     IFDSSQSATPVIAFLPAAGEVHLTRDGRLLSVQNFTMGNHEVDTRGLPYGIYDVEVEV
                     IVNGRVISKRTQRVNKLFSRGRGVGAPLAWQIWGGSFHMDRWSENGKKTRPAKESWLA
                     GASTSGSLSTFSWAATGYGYDNQAVGETRLTLPLGGAINVNLQNMLASDSSWSNIASI
                     SATLPGGFSSLWVNQEKTRIGNQLRRSDADNRAIGGTLNLNSLWSKLGTFSISYNDDR
                     RYNSHYYTADYYQSVYSGTFGSLGLRAGIQRYNNGDSSANTGKYIALDLSLPLGNWFS
                     AGMTHQNGYTMANLSARKQFDEGTIRTVGANLSRAISGDTGDDKTLSGGAYAQFDARY
                     ASGTLNVNSAADGYINTNLTANGSVGWQGKNIAASGRTDGNAGVIFDTGLENDGQISA
                     KINGRIFPLNGKRNYLPLSPYGRYEVELQNSKNSLDSYDIVSGRKSHLTLYPGNVAVI
                     EPEVKQMVTVSGRIRAEDGTLLANARINNHIGRTRTDENGEFVMDVDKKYPTIDFRYS
                     GNKTCEVALELNQARGAVWVGDVVCSGLSSWAAVTQTGEENES"
     gene            4850..6493
                     /gene="ecpD"
                     /locus_tag="ACHKIR_003171"
     CDS             4850..6493
                     /gene="ecpD"
                     /locus_tag="ACHKIR_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial adhesin EcpD"
                     /protein_id="HFO9990866.1"
                     /translation="MRVNLLIAMIIFALIWPATALRAAVSKTTWADAPAREFVFVENN
                     SDDNFFVTPGGALDPRLTGANRWTGLKYNGSGTIYQQSLGYIDNGYNTGLYTNWKFDM
                     WLENSPVSSPLTGLRCINWYAGCNMTTSLILPQTTDASGFYGATVTSGGAKWMHGMLS
                     DAFYQYLQQMPVGSSFTMTINACQTSVNYDASSGARCKDQASGNWYVRNVTHTKAANL
                     RLINTHSLAEVFINSDGVPTLGEGNADCRTQTIGSRSGLSCKMVNYTLQTNGLSNTSI
                     HIFPAIANSSLASAVGAYDMQFSLNGSSWKPVSNTAYYYTFNEMKSADSIYVFFSSNF
                     FKQMVNLGISDINTKDLFNFRFQNTTSPESGWYEFSTSNTLIIKPRDFSISIISDEYT
                     QTPSREGYVGSGESALDFGYIVTTSGKTAADEVLIKVTGPAQVIGGRSYCVFSSDDGK
                     AKVPFPATLSFITRNGATKTYDAGCDDSWRDMTDALWLTTPWTDISGEVGQMDKTTVK
                     FSIPMDNAISLRTVDDNGWFGEVSASGEIHVQATWRNIN"
     gene            6462..7172
                     /gene="ecpE"
                     /locus_tag="ACHKIR_003172"
     CDS             6462..7172
                     /gene="ecpE"
                     /locus_tag="ACHKIR_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpE"
                     /protein_id="HFO9990867.1"
                     /translation="MFRRRGVTLTKALLTVVCMLAAPLTQAISVGNLTFSLPSETDFV
                     SKRVVNNNKSARIYRIAISAIDSPGSSELRTRPVDGELLFAPRQLALQTGESEYFKFY
                     YHGPRDNRERYYRVSFREVPTRNLTKRSPTGGEVSTEPVVVMDTILVVRPRQVQFKWS
                     FDQVTGTVSNTGNTWFKLLIKPGCDSTEEEGDAWYLRPGDVVHQPELRQPGNHYLVYN
                     DKFIKISDSCPAKPPSAD"
     gene            7485..7814
                     /gene="ykgJ"
                     /locus_tag="ACHKIR_003173"
     CDS             7485..7814
                     /gene="ykgJ"
                     /locus_tag="ACHKIR_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFO9990868.1"
                     /translation="MSNLNPCMTCGACCAFFRVSFYWAEADDAGGTIPARLTEQISPF
                     HRCMSGTNQKNPRCIALAGTPGKNACCTIYENRSSTCREFAMSGENGEVNEACNRARA
                     KYGLTPL"
     gene            complement(7826..7992)
                     /locus_tag="ACHKIR_003174"
                     /pseudo
     CDS             complement(7826..7992)
                     /locus_tag="ACHKIR_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885624.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            complement(8063..8677)
                     /gene="yagU"
                     /locus_tag="ACHKIR_003175"
     CDS             complement(8063..8677)
                     /gene="yagU"
                     /locus_tag="ACHKIR_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HFO9990869.1"
                     /translation="MNIFEQTPPNRRRYGLAAFIGLIAGVVSAFVKWGAEVPLPPRSP
                     VDMFNAACGPESLIRAAGQIDCSRNFLNPPYIFLRDWLGLTDPNSAVYTFAGHVFNWV
                     GVTHIIFSIVFAVGYCVVAEVFPKIKLWQGLLAGALAQLFVHMISFPLMGLTPPLFDL
                     PWYENVSEIFGHLVWFWSIEIIRRDLRNRITHEPDPEIPLGSNR"
     gene            complement(8892..9077)
                     /locus_tag="ACHKIR_003176"
     CDS             complement(8892..9077)
                     /locus_tag="ACHKIR_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001468276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9990870.1"
                     /translation="MASGLICHGGFEPHAWGVASLMDVTVLVVASLLVWLLVNRENHY
                     ARGIPQDVQVLHKKAHH"
     gene            9126..9283
                     /locus_tag="ACHKIR_003177"
                     /pseudo
     CDS             9126..9283
                     /locus_tag="ACHKIR_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010835868.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(9298..9373)
                     /locus_tag="ACHKIR_003178"
     tRNA            complement(9298..9373)
                     /locus_tag="ACHKIR_003178"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9338..9340),aa:Thr,seq:cgt)
     gene            complement(9488..10741)
                     /gene="proA"
                     /locus_tag="ACHKIR_003179"
     CDS             complement(9488..10741)
                     /gene="proA"
                     /locus_tag="ACHKIR_003179"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFO9990871.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCSLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAGALAQLQTGPAKVVAVKTEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     SRGPMGLEALTTYKWIGIGDYTIRA"
     gene            complement(10753..11856)
                     /gene="proB"
                     /locus_tag="ACHKIR_003180"
     CDS             complement(10753..11856)
                     /gene="proB"
                     /locus_tag="ACHKIR_003180"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFO9990872.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            12145..13200
                     /gene="phoE"
                     /locus_tag="ACHKIR_003181"
     CDS             12145..13200
                     /gene="phoE"
                     /locus_tag="ACHKIR_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFO9990873.1"
                     /translation="MKKSTLALVVMGIVASVSVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYVDVGATYYFNKNMSAFVDYKINQLESDNK
                     LNINNDDIVAVGMTYQF"
     gene            complement(13239..13640)
                     /gene="crl"
                     /locus_tag="ACHKIR_003182"
     CDS             complement(13239..13640)
                     /gene="crl"
                     /locus_tag="ACHKIR_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFO9990874.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWTSVQIKDAEVVERLEHTLRE
                     FHEKLRELLATLNLKLEPADDFRDEPVKLTA"
     gene            complement(13698..14942)
                     /gene="frsA"
                     /locus_tag="ACHKIR_003183"
     CDS             complement(13698..14942)
                     /gene="frsA"
                     /locus_tag="ACHKIR_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFO9990875.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFSHGAQLPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRYWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKVVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITDWIEKRLC"
     gene            complement(15034..15492)
                     /gene="gpt"
                     /locus_tag="ACHKIR_003184"
     CDS             complement(15034..15492)
                     /gene="gpt"
                     /locus_tag="ACHKIR_003184"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFO9990876.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQNTWIEQPWDMGVVFVPPISGR"
     gene            15753..17210
                     /gene="pepD"
                     /locus_tag="ACHKIR_003185"
     CDS             15753..17210
                     /gene="pepD"
                     /locus_tag="ACHKIR_003185"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFO9990877.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            complement(17267..17619)
                     /gene="prfH"
                     /locus_tag="ACHKIR_003186"
                     /pseudo
     CDS             complement(17267..17619)
                     /gene="prfH"
                     /locus_tag="ACHKIR_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="similar to release factor 1 and 2; frameshifted;
                     incomplete; partial in the middle of a contig; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(17615..17821)
                     /locus_tag="ACHKIR_003187"
                     /pseudo
     CDS             complement(17615..17821)
                     /locus_tag="ACHKIR_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
     gene            complement(18139..18591)
                     /gene="yafP"
                     /locus_tag="ACHKIR_003188"
     CDS             complement(18139..18591)
                     /gene="yafP"
                     /locus_tag="ACHKIR_003188"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO9990878.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFLRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPLIK
                     SESELTVDASITAKPFFERYGFQTVKQQHVECRGAWFTNFSMRYKPQH"
     gene            complement(18588..19643)
                     /gene="dinB"
                     /locus_tag="ACHKIR_003189"
     CDS             complement(18588..19643)
                     /gene="dinB"
                     /locus_tag="ACHKIR_003189"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFO9990879.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(19714..20499)
                     /gene="lafU"
                     /locus_tag="ACHKIR_003190"
     CDS             complement(19714..20499)
                     /gene="lafU"
                     /locus_tag="ACHKIR_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HFO9990880.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAKIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPENKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRAR"
     gene            20471..22183
                     /locus_tag="ACHKIR_003191"
                     /pseudo
     CDS             20471..22183
                     /locus_tag="ACHKIR_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(22401..22898)
                     /gene="rayT"
                     /locus_tag="ACHKIR_003192"
     CDS             complement(22401..22898)
                     /gene="rayT"
                     /locus_tag="ACHKIR_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HFO9990881.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSQLLTTQYQTLRNAIIKVKQ
                     ARPFEINAWVVLPEHMHCIWTLPESDDDFSSRWREIKKQFTHACGLQNMWQPRFWEHA
                     IRNTKDYRHHVDYIYINPVKHGWVKQVSDWPFSTFHRDVAKGLYPIDWAGDVTDFSAG
                     ERIIL"
     gene            complement(23075..23824)
                     /gene="yafL"
                     /locus_tag="ACHKIR_003193"
     CDS             complement(23075..23824)
                     /gene="yafL"
                     /locus_tag="ACHKIR_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000093946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO9990882.1"
                     /translation="MSSFLHGRFLHPGVFSLCVLLPLLASATTSHISFSYAARQRMQN
                     RARLLKQYQTHLKKQASYIVEGNAKSRRALRQHNREQIKQHPEWFPAPLKASDRRWQA
                     LAENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDQGFDCSGLVFYAYNKILE
                     AKLPRTANEMYHYHRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDGQFIESPRT
                     GETIRVSRLAEPFWQDHFLGARRILTEETIL"
     gene            24136..24876
                     /gene="dpaA"
                     /locus_tag="ACHKIR_003194"
     CDS             24136..24876
                     /gene="dpaA"
                     /locus_tag="ACHKIR_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan meso-diaminopimelic acid protein
                     amidase"
                     /protein_id="HFO9990883.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(24847..25614)
                     /gene="yafJ"
                     /locus_tag="ACHKIR_003195"
     CDS             complement(24847..25614)
                     /gene="yafJ"
                     /locus_tag="ACHKIR_003195"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HFO9990884.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(25730..26308)
                     /gene="lpcA"
                     /locus_tag="ACHKIR_003196"
     CDS             complement(25730..26308)
                     /gene="lpcA"
                     /locus_tag="ACHKIR_003196"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HFO9990885.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            26548..28992
                     /gene="fadE"
                     /locus_tag="ACHKIR_003197"
     CDS             26548..28992
                     /gene="fadE"
                     /locus_tag="ACHKIR_003197"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0033539 - fatty acid beta-oxidation using
                     acyl-CoA dehydrogenase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HFO9990886.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            complement(29035..29481)
                     /gene="ivy"
                     /locus_tag="ACHKIR_003198"
     CDS             complement(29035..29481)
                     /gene="ivy"
                     /locus_tag="ACHKIR_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HFO9990887.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            29662..30432
                     /gene="yafV"
                     /locus_tag="ACHKIR_003199"
     CDS             29662..30432
                     /gene="yafV"
                     /locus_tag="ACHKIR_003199"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HFO9990888.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNERVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMMALREYREKFPAWRDADEFRLW"
BASE COUNT         7605 a         7848 c         7762 g         7273 t
ORIGIN      
        1 ttcctaacac ctcagggcaa agttcctggc taatataaaa tgcaagtaag aattgaacgt
       61 tatattgcca ataaccttat gaaactgaat gtctttttct tcttatcaaa aaagcaatat
      121 tttcattttt tgtaaatatt gacttaacca tggaattcat tttctgttca catattgaca
      181 ctcatcagga aaaaaacata aatttaaacc taatcgaaat aattaaaact taatctcgtt
      241 taacctatat tgatatgtgc tacgtatctt atttacttcc gatttactaa agaaactgaa
      301 tgtacctgta aaaattacag gtttggaaag tagtgacatg gcaaaatgat tacagcaggg
      361 actatgaggt taaaaaccat atggaatgtc aaaaccgttc tgataaatac atctggtctc
      421 cccatgacgc ctacttctat aaaggactat ctgaactgat tgtggatatc gacagattaa
      481 tttatctatc gctggagaaa atcagaaaag atttcgtgtt tatcaatctc aatacggatt
      541 ctttaactga gtttataaac cgtgataatg agtggttatc cgcggtaaag gggaaacagg
      601 tcgtattgat tgcggccaga aagtcagaag ccttagcaaa ttattggtat tacaacagca
      661 atattagggg cgtggtatac gctggactga gtcgtgatat tagaaaagaa ctggcctatg
      721 tgattaatgg caggttcctg agaaaagata ttaagaaaga taaaatcact gaccgggaaa
      781 tggaaattat ccgcatgacg gctcagggaa tgctgcctaa atcgattgcc agaattgaaa
      841 attgtagtgt gaagacagtg tatacccatc ggcggaatgc agaggccaag ctgtactcaa
      901 aattatataa gttggttcag taaactccaa gcaagttagt tttaaaaatg actcaccggg
      961 acttcatgtc ctcaattcaa ctcgggaaga aaagcaatga aaaaaaaggt tctggcaata
     1021 gctctggtaa cggtgtttac cggtacaggt gtagcgcagg ctgctgacgt aacagctcag
     1081 gctgtagcga cctggtcagc aacagccaaa aaagacacca ccagtaagct ggttgtgacg
     1141 ccactcggta gcctggcgtt ccagtatgcc gaaggcatta aaggttttaa ctcacaaaaa
     1201 ggtctatttg acgtggctat cgagggtgac tcaacggcta ccgcctttaa actgacctca
     1261 cgccttatca ccaacacctt aacccagttg gatacctcag gttccacact gaatgtgggc
     1321 gtggattata acggcgcggc agtcgaaaaa actggcgata ccgtgatgat cgataccgcc
     1381 aacggcgtac tgggcggcaa ccttagccca ctggctaacg gttacaatgc cagcaatcgt
     1441 accaccgcac aggatggttt cactttctcc atcatcagcg gcaccaccaa tggtaccacc
     1501 gcagtaaccg attacagcac tctaccggaa ggcatctgga gcggcgacgt tagcgtacag
     1561 ttcgacgcta cctggaccag ttaatctctc tgatgtacca gcaggggtac ccccctgctt
     1621 tattcctgga tggaattgtt atgaaaaagc accttctgcc tctcgctctg ctgttttccg
     1681 gaatatcacc ggcccaggcg ctggatgtcg gcgatatatc atcgtttatg aacagtgaca
     1741 gcagcacgct aagcaaaacg atccaaaaca gtaccgacag tggccgcctc atcaatatcc
     1801 gtctcgaacg gctctcttca ccgcttgacg acgggcaggt tatcgcaatg gacaagccgg
     1861 atgagttgct actcactccc gccagcttgc tgctacccgc ccaagccagc gaagtgatcc
     1921 gcttcttcta taagggacca gcagatgaaa aagagcgcta ctaccgcatt gtctggtttg
     1981 atcaggccct cagtgatgcg cagcgcgata atgccaaccg cagcgctgtg gccactgctt
     2041 ccgcccgcat cggcaccatt ctggtcgtcg caccccgcca ggcaaactac cactttcagt
     2101 acgccaacgg ctccctgaca aatacaggaa atgcgacgct gcggatcctc gcctacggcc
     2161 cctgcctgaa agccgccaat ggtaaggagt gtaaagagaa ttactacctg atgccgggca
     2221 agtcgcgtcg ttttacccgc gtggacacgg cggataacaa aggacgggtt gcactttggc
     2281 agggtgataa gttcattccc gtgaaataga tagctgtgca gatggataac gacaatgcct
     2341 ttacgacggt tctccccagg actgaaagcc cagtttgcct tcggcatggt ctttttgttc
     2401 gttcagcccg atgccagcgc tgctgacata agtgcgcagc aaataggtgg ggtgattatt
     2461 ccgcaggcct tcagtcaggc gctccaggac ggcatgagcg tcccgcttta tattcatctc
     2521 gccggtagcc agggtcgcca ggacgatcag cgaatcggca gcgcttttat ctggttggat
     2581 gatggacagt tacgcatccg aaaaatacag ctggaagaga gtgaagataa cgccagtgtc
     2641 agcgaacaaa ctcgacagca gctgatgact ctggccaacg ccccgttcaa tgaggccctt
     2701 accatccccc tgactgacaa cgcgcagttg gatctcagct tgcgccaact gctgctgcag
     2761 ctggtggtca agcgcgaagc gctgggcacc gtactacgct cacgtagcga agacatcggg
     2821 cagtccagtg ttaacaccct cagcagtaat ctgagctata acttcggcgt ctataacaac
     2881 cagttgcgta acggcgggag caacacctcc agctatctgt cgctgaataa cgttactgcg
     2941 ctgcgcgaac atcacgtggt gctcgacggc tcactgtacg ggattggtag cggtcaacag
     3001 gacagtgaat tatataaagc gatgtatgaa cgcgattttg ctggtcaccg atttgccggt
     3061 ggaatgctcg acacctggaa cttgcagtcc ttagggccga tgaccgccat ttcagcaggg
     3121 aagatttacg gcctttcctg gggaaaccag gccagctcca ccatcttcga cagcagccag
     3181 tcggccacgc cagtgatcgc ctttttaccg gcggcgggcg aagtacatct cacccgtgat
     3241 gggcggctac taagcgttca gaacttcacc atgggcaatc atgaagtgga tacccggggt
     3301 ctaccgtacg gtatttacga tgtggaagtt gaggtaatcg ttaacggtcg cgtgatcagc
     3361 aaacgcaccc agcgggtcaa taagctgttt agccggggac gtggcgtcgg tgcaccgctg
     3421 gcgtggcaga tatggggcgg tagctttcat atggatcgct ggtcggaaaa cgggaaaaag
     3481 acgcgaccag ccaaagagag ttggctggca ggtgcctcga cctccggctc actgagtacg
     3541 tttagctggg cggcaacggg atatggatac gataatcagg cggtgggtga aacccgtctg
     3601 acgctgccgc ttgggggggc gatcaacgtt aacctgcaaa acatgctggc cagtgacagc
     3661 tcatggagca acatcgccag catcagcgcc actctacctg gaggcttcag ttcgctgtgg
     3721 gttaaccagg aaaaaacccg cattggcaat caattgcgac gtagcgatgc cgacaaccgt
     3781 gcaatcggcg gcacactcaa cctgaactca ctgtggtcga agctgggtac gttcagcatc
     3841 agctacaatg atgaccgccg ctacaacagc cattattaca cggcagatta ctatcaaagt
     3901 gtctacagcg gtacctttgg ttcgcttggc ctgcgggccg gtattcagcg ctataacaac
     3961 ggcgacagca gcgccaatac agggaaatat atcgctctcg atctctcgct accactgggc
     4021 aactggttta gcgcagggat gacccatcaa aacggctaca ccatggcaaa cctgtcagca
     4081 cgcaaacagt ttgatgaagg aaccattcgc actgttggtg ccaatctgtc acgagccatc
     4141 tccggcgata ccggtgatga caaaaccctc agcggtgggg cgtatgcaca gttcgacgct
     4201 cgttacgcca gcggaacgct gaacgtcaat agcgcggcgg acggctacat caatactaat
     4261 ttgaccgcca acggcagcgt cggctggcag ggtaaaaaca tcgctgccag cgggcggacc
     4321 gatggcaacg ctggggtgat attcgacact ggcctggaga acgatggtca gatcagcgcc
     4381 aaaatcaacg ggcggatttt cccgcttaac ggcaagcgta actatctccc gctctctccc
     4441 tatggaagat atgaggtgga gttgcagaac agcaaaaact cactcgacag ttacgatatc
     4501 gtcagcggtc gcaaaagtca tctgactctc tatccaggca atgtcgctgt cattgagcca
     4561 gaggtgaagc agatggttac cgtctccggt cgtatccgtg cggaagacgg cacactgctg
     4621 gctaacgcac ggattaacaa ccatatcggc cgaacccgaa ccgatgaaaa cggcgagttt
     4681 gtcatggacg tggataagaa ataccccact atcgattttc gctacagtgg caataaaacc
     4741 tgcgaagtgg cactggaact caaccaggcg cgcggtgccg tctgggtcgg tgatgtggtc
     4801 tgcagcggcc tctcatcgtg ggcggcggtg acgcagacag gagaagagaa tgagagttaa
     4861 cctactgata gcgatgataa tctttgcgct aatctggcct gcaaccgcgc tcagagcggc
     4921 agtgagcaaa acaacctggg cggatgctcc ggcacgcgag tttgtgtttg tcgaaaacaa
     4981 ctcagacgac aactttttcg tcactcctgg cggggcgctg gatccgcgcc tgaccggtgc
     5041 caaccgctgg accggtttaa aatacaatgg ttcaggaacc atctatcagc aaagcctcgg
     5101 ctacattgat aacggttaca acaccggcct ttataccaac tggaagtttg atatgtggct
     5161 ggaaaattca ccagtttcat ctcctttaac tggcttgcgc tgcatcaact ggtacgcagg
     5221 gtgtaatatg accaccagtc ttatcctgcc gcaaaccacc gacgccagtg gattttatgg
     5281 cgcgacggtg accagcggcg gcgcgaaatg gatgcacggc atgttgtcag acgcgtttta
     5341 ccagtatctg caacaaatgc ccgtcggcag cagctttaca atgaccatca atgcctgcca
     5401 gacctctgtg aactatgacg ccagcagcgg cgcacgctgt aaggatcagg cctccggcaa
     5461 ctggtatgtt cgcaacgtca cccatacgaa agcagcaaat ctgcggttga taaataccca
     5521 ctcgctggcg gaagtattta tcaacagcga cggagtaccg actctgggcg aagggaacgc
     5581 cgactgccgg acgcaaacca tcggcagccg ttcaggatta agttgtaaga tggttaacta
     5641 taccctgcaa acaaacggac tcagcaacac ctcaatccat atattcccgg cgatcgccaa
     5701 ctcgtcgtta gcctcggccg tcggggcgta cgatatgcag ttcagtctga atggcagttc
     5761 atggaaaccg gtgagcaata ctgcctatta ctacaccttc aacgagatga agagcgcaga
     5821 ctcgatctat gttttcttct cgagcaactt ctttaaacag atggtgaacc tcgggatcag
     5881 cgatatcaac accaaagatc tattcaactt tcgctttcag aacaccacat caccggagtc
     5941 tggctggtat gaattttcta cctccaacac gctgattatc aaaccccgtg atttcagcat
     6001 cagtattatc tccgatgaat atactcagac accgtcgcgg gagggatatg ttggcagcgg
     6061 cgagtcggca ctcgatttcg gctatatcgt aaccaccagc ggtaaaacag ctgccgacga
     6121 agtgctgatc aaggtgaccg gacccgcgca ggtgattggc gggcgctcct attgtgtctt
     6181 cagctccgat gacggtaagg cgaaagtacc gttcccggcg acgctttcct ttattacccg
     6241 caacggagct acaaaaacct acgatgccgg gtgcgatgat agctggcggg atatgaccga
     6301 tgcgctgtgg ttgaccacac cgtggactga tatctctggc gaagtggggc agatggataa
     6361 gaccacagtc aaattttcga ttccaatgga taacgccatt tctctgcgta cggtagatga
     6421 taacggctgg tttggcgaag tcagcgcttc aggagagatt catgttcagg cgacgtggcg
     6481 taacattaac taaggccctg ctgacagtgg tctgtatgct ggcggcacct ttgacacagg
     6541 cgatttcggt cggcaatctg actttttcac tgccgtccga gactgacttt gtcagcaaac
     6601 gtgtcgtgaa taacaacaaa agcgcgcgga tataccgtat tgccatcagt gctattgata
     6661 gcccgggcag cagtgaattg cgcacccgac cggtggatgg tgaactactt ttcgcccccc
     6721 gccagctggc gttgcagact ggtgagagcg agtattttaa attttactat catggtccac
     6781 gggataaccg cgagcgctac taccgggtct catttcgcga ggtccccact cgtaacctga
     6841 caaaacgcag cccaaccggc ggtgaggtca gcacggagcc ggtggtggtg atggatacca
     6901 ttctggtagt acgaccacgt caggttcagt ttaaatggtc cttcgatcag gtgaccggaa
     6961 cggtaagtaa caccggcaac acgtggttta agctactgat taaaccagga tgtgattcga
     7021 ccgaagagga aggcgatgcc tggtatctac gtccgggaga cgtggttcat cagcctgagt
     7081 tacgtcagcc ggggaatcat tatctggtct ataacgacaa attcattaag attagcgatt
     7141 cctgtccggc taagccccct tcggcggact aagacttatc catcggacga gggaagaatt
     7201 ccacctcaga gactcaaaaa tcgtttaaat gatgtggaag cgatcgtcaa tgtcacctgt
     7261 aacgatgagc tttctcatat gcacaccaca gtgttcagga gacacggaaa ggtaccaggc
     7321 ggcctgctaa cgagtggttt cttcactaat taggacgtaa aatcggtaac ggctggaaat
     7381 cattcaatac tcacactatc gaaagtacac cagccaaccg cgatacatcc ctgcatacga
     7441 cgtaccgcag ttctctttac gatttatagc tgtactggtg aattatgagc aatctgaatc
     7501 catgcatgac gtgtggtgcc tgttgtgcat ttttccgcgt ctctttttac tgggccgaag
     7561 ctgacgatgc tggcggtacc attcccgcca ggctcactga acaaatatcc ccttttcacc
     7621 gatgcatgag cggtactaat cagaaaaatc cccgatgtat tgcccttgca ggaacaccgg
     7681 gcaaaaatgc ctgctgcacg atatatgaaa atcgatcgtc cacatgcaga gaattcgcca
     7741 tgtctggtga gaacggagaa gtcaatgagg cttgcaatcg tgcaagagct aaatacgggc
     7801 taacaccgtt ataaatatac aataattaat tgcattgccc cgccagctgt aatagcgggg
     7861 cttcgttttt cagggacaac aaaacctgaa acttcttcca ttttccagca acaatgcctc
     7921 ttcttctgta gcgttctccg gatctacatc atcctgaagt agcgcgatat gatcactgac
     7981 tgtccgcatc agaatatgaa aagcaacgtt tttaacctat aaaaatggcg ctatatttgc
     8041 gccattttta tcattcaaag cattatctat ttgagcctaa agggatctca ggatctggct
     8101 catgggtaat tctgtttcgt aaatctctgc gaataatttc aatagaccag aaccagacta
     8161 aatgtccaaa aatttcagag acattctcat accacggaag atcaaagaga ggtggcgtaa
     8221 gtcccatgag agggaatgaa atcatatgaa caaaaagttg cgctaaagca cctgccagta
     8281 agccctgcca aagtttaatt ttcggaaata cttcggcgac cacacagtaa ccaacggcaa
     8341 aaactatcga aaagattatg tgcgttacac caacccagtt aaagacatgc ccggcgaagg
     8401 tataaacagc cgaatttgga tctgtcagtc ccaaccaatc ccgaagaaaa atgtacggag
     8461 gattgagaaa gttacgggag caatcaattt ggcctgcagc cctgattaat gattccggac
     8521 cacacgctgc attaaacata tcaaccggac tacgtggtgg caatggaact tctgcccccc
     8581 acttaacaaa tgcggaaaca acgccagcaa tcagcccaat gaatgcagca agaccataac
     8641 gtctgcggtt tggtggagtt tgttcaaata tattcatatc taccctgctt gtaccattat
     8701 gttatacacc tctttaggag tattcataaa acaaggcaaa tgtaaagaac tgtattgttt
     8761 tgtataacca catggttacc taatcgccaa ttaatataag ctccattatt tctccatatt
     8821 ttcatgttag aagtgacaag gagaaatgta atgtttctct cagagaatat aaatcattcc
     8881 ttttgcccac cttaatggtg ggctttttta tgcaaaacct gaacgtcctg tggtattcct
     8941 ctggcataat gattttcgcg attaaccaac aaccagacca gcaaagacgc caccacaagc
     9001 acagtaacat ccatcaggct tgccacaccc catgcgtgcg gctcaaagcc gccatggcat
     9061 attaatccgc ttgccatttt cgaacagctg attggtttgg tccggtgttt ccctgcttgc
     9121 acttaaaaat gagcggatgg gaaactctcg aaatggttcg tcgatatgct cacctcgcac
     9181 ctaatcacct taccgaacac gcacggtaaa tagattcgat cctgaaccca tcggtcccaa
     9241 atctgtccca ataaaaaata aggaaggtat tagtgatgtg taacttattg atttaaatgg
     9301 tgccgataag aggagtcgaa cctccgacct tcgcattacg aatgcgctgc tctaccaact
     9361 gagctatatc ggccctgaaa ggacatgttc acgaacgtga atcacggtgg acaaggttaa
     9421 aactaaccgg gcgatgcgtc aatggccttg tgaatcaaat ggctactttt gcatcacccg
     9481 gttttattta cgcacgaatg gtgtaatcac caatgccgat ccacttgtaa gtggtcagtg
     9541 cttccagccc cattgggcca cgcgagtgga gtttttgtgt gcttaccgcc acttccgcac
     9601 ccagtccaaa ctggccgccg tcggtaaaac gcgtagaggc gttaacgtaa acagcggagg
     9661 aatctacttc gttaacaaaa cgctgggcgt tgcgcatatc gcgggtcagg atcgcatcgg
     9721 agtgttgcgt gccgtgttca cgaatatggg cgatggcatc gtcaagatcg ctgacgattt
     9781 tgacgttcaa atctaatgac agaaactcat cgtcatactc ttcggtttta acagcaacca
     9841 ccttcgcggg gcctgtctgc aactgcgcca gtgcacctgc atcagcgtgt aacgtcacgc
     9901 cactttccgc catttgtttg cttaatgcgg gcaggaaact atcggcgatg tttttattca
     9961 ccagcaacgt ttcaaccgta ttacatgtgc tcggtcgctg agttttcgcg ttgacgatca
    10021 ctttcagggc ttcagcgatc tctgcacttt catcaacgta aatatggcat acgcctatac
    10081 cacctgtgat caccgggatc gtcgactgtt cgcggcacag tttatgcagg ccagcgccac
    10141 cgcgcgggat cagcatgtcg atgtatttat ccatacgcag catttcactg accagcgcgc
    10201 ggtcaggatt atcaatcgcc tgcacggcac ccgccggtaa gctgcaggat ttcagggcgt
    10261 cctgaatcac cgccaccgtt gcagcgttag tgcgacacgt ttctttgccg ccgcgcagga
    10321 tcaccgcgtt acctgttttc aggcacagcg aagcgacatc taccgtcacg ttcgggcgcg
    10381 cttcataaat cacgccaatc acccccagcg gcacgcgacg gcgctccaga cgcagaccac
    10441 tgtccagtac gccgccatcg attacctgcc ccaccggatc ggcgagatta cacacctgac
    10501 gcacatcgtc ggcgatgcct ttcagccgtg cgggcgtcag tgccagacgg tcaagcatcg
    10561 cttcgctaag gccattggca cgcgcgtcag caacatcctg ggcgttagcg ttgaggatga
    10621 tttcgctttg tgcttccagt tcatcggcga ttttttccag cacacgattt ttttcgcggc
    10681 tggagagttg cgctaattta tacgaggctt gctttgcggc aatgcccatt tgttccagca
    10741 tcggccagct ccttaacggg taatcatgtc atcacggtga acggcaaccg ggccatattc
    10801 atatcccagt attgcatcaa tttcttgcga gtggtgtccg gcaatgcggc gtaatgcatc
    10861 gctgttgtaa cgactgacgc cgtgggcgat atcgcgacct tcgaggttgc aaatgcggat
    10921 gacttcacca cgcgagaaat tgccagttac gctcttaatg cctttcggca acagggagct
    10981 gccgcgttca agaatggcgg cggttgcccc ttcatctacc gtgatttcac ccgccggcgg
    11041 cgcaccgaaa atccagcgtt tacggttttc aagcggagtc gcctgggcat ggaacagcgt
    11101 accgacggaa atgccttcca tcacatcacc aataacgccc ggcttgctgc ccgcggcaat
    11161 aatggtgtcg atacccgcac ggcaagccac gtccgcggcc tgcaatttgg tgctcatacc
    11221 gccagttccg aggcctgaaa cgctatcacc ggcaatcgcg cgcagtgcgt catcaatgcc
    11281 gtacacatct ttaatcagtt ctgcctgcgg attgctgcgc gggtcagcgg tatacaaacc
    11341 tttttgatcg gtcagcagca acagtttatc ggcacccgcc agaatcgccg ccagcgcaga
    11401 aaggttatcg ttatcaccga ccttaatctc tgccgtagcg acagcatcgt tctcattaat
    11461 taccggaacg atattgttat cgagcaacgc acgcagggta tcgcgggcgt tcaggaagcg
    11521 ttcacgatct tccatatcag cacgggtcag caacatctgc ccgacatgaa tgccgtaaat
    11581 tgaaaacagc tgttcccaca gttgaatcag tcgactctgc cctaccgccg ccagcagctg
    11641 ttttgaagcg atagtcgctg gcaattccgg gtaccccagg tgctcacgtc cggcggcaat
    11701 cgcacccgac gtcacaataa caatccgatg cccggcggca tgtaactgcg cgcactggcg
    11761 aacaagttca acgatatggg cacggttcag gcggcgcgat ccgcctgtta gcacactggt
    11821 gccgagtttt accaccagcg tctggctgtc actcatgatt ctctgccatt caattttagg
    11881 aaaaaatgat atcaaacgaa cgttttagca ggactgtcgt cggttgccaa ccatctgcga
    11941 gcaaagcatg gcgttttgtt gcgcgggatc agcaagcgta gcggcagttg tttacgcttt
    12001 tattacagat ttaataaatt accacatttt aagaatatta ttaatctgta atatatcttt
    12061 aacaatctca ggttaaaaac tttcctgttt tcaacgggac tctcccgctg aatattcgcg
    12121 cgttaattaa aatcaggaat gaaaatgaaa aagagcactc tggcattagt ggtgatgggc
    12181 attgtggcat ctgtatccgt acaggccgca gaaatatata acaaagacgg taataaactg
    12241 gatgtctatg gcaaagttaa agccatgcat tatatgagtg ataatgacag taaagatggc
    12301 gaccagagtt atatccgttt tggttttaaa ggcgaaacac aaattaacga tcaattgacc
    12361 ggttatggtc gctgggaagc agagtttgcc ggtaataaag cagagagtga tactgcacag
    12421 caaaaaacac gtctcgcttt tgccgggttg aaatataaag atttgggttc tttcgattat
    12481 ggtcgtaacc tgggcgcgtt gtatgacgtg gaagcctgga ccgatatgtt cccggaattt
    12541 ggtggcgact cctcggcgca gaccgacaac tttatgacca aacgcgccag cggtctggcg
    12601 acgtatcgga acaccgactt cttcggcgtt atcgatggcc tgaacttaac cctgcaatat
    12661 caagggaaaa acgaaaaccg cgacgttaaa aagcaaaacg gcgatggctt cggcacgtca
    12721 ttgacatatg actttggtgg cagcgatttc gccatcagtg gagcctatac caactctgat
    12781 cgcaccaacg agcagaacct gcaaagccgt ggcacaggca agcgtgcaga agcatgggca
    12841 acaggtctga aatacgatgc caataatatt tatctggcaa ctttctattc tgaaacacgc
    12901 aaaatgacgc caataactgg cggctttgcc aataagacgc agaactttga agcggtcgct
    12961 caataccagt ttgactttgg tctgcgtcca tcgctgggtt atgtcttatc gaaagggaaa
    13021 gatattgagg gcatcggtga tgaagatctg gtcaattatg tcgatgttgg tgctacgtat
    13081 tatttcaaca aaaatatgtc agcgtttgtt gattataaaa tcaaccaact ggaaagcgat
    13141 aacaaattaa atattaataa tgatgatatt gtcgcggttg ggatgacgta tcagttttaa
    13201 tgaatattgc cggatgtgat gcatccggca catttcattc acgccgttaa cttcaccggt
    13261 tcatcacgaa aatcatctgc cggttccagc ttcagattca gcgtcgccag cagctcacgc
    13321 agcttctcgt gaaattcacg cagggtgtgc tccagtcgtt caaccacttc ggcatctttt
    13381 atctgaacac tcgtccagtc gcctgcttta tcgaacagac caaactggta actgtaggta
    13441 aaacgggatt cctgcgcttc cagttccatc caccagcccc agaactcacg cacttccggt
    13501 gccggtttca cgttgacgca tacagccaga caatcgaaaa agaatcgatt atcttcgcac
    13561 ttaccttcac gaatatacgg gcctagtgcg gtaaattttt tgatcaatct gctcttcggg
    13621 tgtccactcg gtaacgtcat tgcaatctcc tgttgtgatg caactgtttt accaaattgg
    13681 taaaatttag caaattttta acacaaacgt ttttcgatcc agtctgtgat ttcctgaagg
    13741 cctttgtcaa aattccgata caccgggtta aatgggatct ctaataattt accgtcagca
    13801 gatgacgacg tgattaagcg tgagtcctct tccgggctga acggatcgtt cttccagtag
    13861 cctgataaca ttggcgttgg gcagcgacgt cccagcaatc cttgcacttt taatgaatag
    13921 cgattcaact ccactcgcaa cgcttcatcg gaagcatcat gcatccctaa acgactggcc
    13981 agaacgtcaa gatacatttc cggcacctgt tgctggcact taaaatcact caacagggta
    14041 tgaactaccg gaccaagaca ggcaaccact ttcagacgcg gcgattcaag gtatgccaga
    14101 cgcacggcaa cgttagcgcc gaaacggaaa ccaaaggccg cgacgcgagt gtgatccacc
    14161 cacggtacgt taggcagcgc ctttaaaacg tgctgatgca acaggctgga gtcctgggtg
    14221 agcttccatt ttgaagaaaa gcccaccgac ggcatatcaa tagtcagcat agcaatgccg
    14281 cgcggcgcaa aataacgttc atacaggctg taatagtccg tctgcatcgc atccagacca
    14341 ccacacatta atactgtcgg gaacgggcca tcgcctttcg gcatatgcaa aaagccggtg
    14401 atgggcgcac cgccgggtac ggtaaactcc atctgccgca tcgtgcccgg cagacgctga
    14461 gcggcctctt cataggcgcg gtttgacaaa gcctgcgctt gctcggccag gtcatctcct
    14521 ttcagatgag gataggcagc aatgttgtac aacgtagccg catgcagcca gtatcgaccg
    14581 ctgagttgcg gatcttcttc cgcacaggct ttttgttgcc acaccatcgc ctgggtagcc
    14641 cactcataaa tccagttgcc gccgcggtag ccaatcaccg tatcgtataa atcatcgtcg
    14701 gtacgttcgg catcgctcat cacaatacgc gcctggacgt cgaggatttc acgggggtca
    14761 atgccgcgcc agatccacat cagacggtta atcatgcgat accagtgagg gatggtttta
    14821 ccatcaaggg ccgactgcac aggcagttgt gcgccgtgac taaagcggcg gactagcgtc
    14881 gaggtttcag gatgtttaaa gcggggtttg aacagggttt cgctcaggtt tgcctgtgtc
    14941 atggatgcag cctccagaat acttactgga aactattgta acccgcctga agttaaaaag
    15001 aacaacgccc ggcagtgcca ggcgttgaaa agattagcga ccggagattg gcggcacgaa
    15061 tacgacgccc atatcccacg gctgttcgat ccaggtattt tgcgggatat caacaacata
    15121 atcatcaacc agcggacgac cagccggttt tgcgaagatg gtgacaaagt gcgcttttgg
    15181 atacatttca cgaatcgcaa ccgcagtacc accggtatct accaggtcat caataacgat
    15241 gaagccttcg ccatcgcctt ctgcgcgttt cagcacttta agctcgcgct ggttgtcgtg
    15301 atcgtagctg gaaatacaaa cggtatcgac atgacgaata cccagttcac gcgccagtaa
    15361 cgcacccggt accagaccgc cacggcttac ggcaataatg cctttccatt gttcagaagg
    15421 catcagtcgg cttgcgagtt tacgtgcatg gatctgcaac atgtcccagg tgacgatgta
    15481 tttttcgctc atgtgaagtg tcccagcctg tttatctacg gcttaaaaag tgttcgaggg
    15541 gaaaataggt tgcgcgagat tatagagatc tggcgcacta aaaaccagta tttcacatga
    15601 gtccgcgtct ttttacgcac tgcctctccc tgacgcggga taaagtggta ttctcaaaca
    15661 tatctcgcaa gcctgtcttg tgttgacaac attttctgct aaccctgtga cctgcaatac
    15721 tgttttgcgg gtgatcgaca aggagactta acgtgtctga actgtctcaa ttatctccac
    15781 agccgctgtg ggatattttt gccaaaatct gttctattcc tcacccgtcc tatcatgaag
    15841 agcaactcgc tgaatacatt gttggttggg caaaagagaa aggtttccat gtcgaacgcg
    15901 atcaggtagg taatatcctg attcgtaaac ctgctaccgc aggtatggaa aatcgtaaac
    15961 cggtcgtctt gcaggcccac ctcgatatgg tgccgcaaaa aaataacgac accgtgcatg
    16021 acttcaccaa agatcctatc cagccttata ttgatggcga atgggttaaa gcacgcggca
    16081 ccacgctggg tgcggataac ggcattggta tggcctctgc actggcggtt ctggctgacg
    16141 aaaacgtggt tcacggcccg ctggaagtgc tgctgaccat gaccgaagaa gccggtatgg
    16201 acggtgcttt tggcttacag agcaactggt tgcaggctga tattctgatt aacaccgact
    16261 ccgaagaaga aggtgaaatc tacatgggtt gtgcgggagg tatcgacttc acctccaacc
    16321 tgcatttaga tcgtgaagcg gttccagctg gctttgaaac cttcaagtta accttaaaag
    16381 gcctgaaagg cggtcactcc ggcggtgaaa tccacgttgg cctgggtaat gccaacaaac
    16441 tgctggtgcg cttcctggcg ggtcatgcag aagaactgga cctgcgcctt atcgatttca
    16501 acggcggcac actgcgtaac gccatcccgc gtgaagcctt tgcgaccatt gctgtcgcag
    16561 ctgataaagt cgacgctttg aaatctctgg tgaataccta tcaggagatc ctgaaaaacg
    16621 agctggcaga gaaagagaaa aatctggcct tgttgctgga ctctgttgcg aacgataaag
    16681 ctgccctgat tgcgaaatct cgcgatacct ttattcgtct gctgaacgcc accccgaacg
    16741 gtgtgatccg caactccgac gtggcaaaag gtgtggtcga aacctccctg aacgtcggtg
    16801 tggtgaccat gactgacaat aacgtagaaa ttcactgcct gatccgttct ctgatcgaca
    16861 gcggtaaaga ctacgtggtg agcatgctgg attcgctggg taaactggct ggcgcgaaaa
    16921 ccgaagcgaa aggcgcatat cctggctggc agccggacgc taattctccg gtgatgcatc
    16981 tggtacgtga aacctatcag cgcctgttca acaagacgcc gaacatccag attatccacg
    17041 cgggcctgga atgtggtctg ttcaaaaaac cgtatccgga aatggacatg gtttctatcg
    17101 ggccaaccat caccggtcca cactctccgg atgagcaagt tcacatcgaa agcgtaggtc
    17161 attactggac actactgact gaactgctga aagaaattcc ggcgaagtaa ttatttgatt
    17221 tgctgccgga tggcgtttaa tcgccttccg gcagtttcat ccttcattat ccttcgataa
    17281 aagccatccc tgtgaacgtt cgtcgcgggt tgccacgttc aatctggtga tggaacattc
    17341 gccgctgcga tttcagcgcc gcgctatttt cctgttgctg ttgttccagc ttccaggcaa
    17401 tcagcaatcg cgccagccgc ttgttagcat gcccagaaac cagtttttgc gcccatgatg
    17461 aggccgatac ggactcggac aaatacactg aatagtgccg caccaacttt cgcttaatgc
    17521 ccatgcatta tcgccatcca gagaaatcag cgccgaacgc agcgtgtcag agtagcggcc
    17581 cgtttctgtt tccagcaccg ttaccgcgac gtcttgccgg gtaaaaacga ccaacagaga
    17641 agaacgctgc gccaatcggg tagccgcgtc cgggatgtaa atctggcatc cccacaacgc
    17701 gctgcatgtt gggtaaattt gccgtggtgt gtaattgttg gatcgctaaa ctctcgatcc
    17761 acaggtcatc agatgcgatg gtaaataccg catcggataa gggacgaata tatttgccca
    17821 tgtatcattc catttatact cgtcatactt caagttgcat gtactgcgac cgcgttcgtt
    17881 caccccagtc acttacttta gtaagctcct ggggattcac tcgcttgtcg ccttcctgca
    17941 actcgaatta tttagagtat ataaatataa aaatcaggaa atggcagacg aatccagggc
    18001 aagaaaaaaa gaattaatcc tggaaaggtc tgcaaagagt cattgataaa ggatttgtca
    18061 tgatttacct ccttgcagtt tgtggaataa gaaaacgcgc acataccagg cgggtgttat
    18121 tttcattgca agctggattt aatgttgcgg tttatatcgc atagaaaaat tagtaaacca
    18181 cgctcccctg cattcaacgt gctgctgctt aactgtctga aaaccataac gttcaaaaaa
    18241 gggttttgcg gttatgctgg cgtccaccgt aagttcggat tcagacttaa tcaaaggttt
    18301 taacaaggcg ctggcaaccc cgcggcgggt gtattcaggg tcaacaaata acatatcgat
    18361 atagtgttca acgcaggtaa taaagccgac cagttttgca ttaatgactg caacccgcac
    18421 ttgtgatttc gcgagtttct ccttccagcg agattcgtca atctgcgccc aggcggcaat
    18481 ttgttgtggt gaataatgct gactggcggt catcgtaacc gctctaagga aaatagcgca
    18541 tagttgctga aaatcgccag gctgatagtc tcttatttgt atgttattca taatcccaac
    18601 accagttgtc tttccatttg cgggtcaagt aacgtcacat gcagccccac cagtcgcaca
    18661 ccgcgcccgc cgcggcgttc atcccaggtt ttacgcgcag tggcgattag atcagcttta
    18721 ttcagccgtg gccagacgtg ctcctgagta gtttgctgaa aatcgtcgaa ctttaatttc
    18781 actccctgac gggcgatcag taaatcaggt ttcacctttg ccagacggcg ttcaagttca
    18841 ggatacagcc gctcgataat cgcttcacat tcagaccagt gatgaatatc ttccgccatc
    18901 gtgcgttcca cgccgacgga ttttcgcaac cgttcactgt taacgtcgcg ctcgtcaatc
    18961 ccctgactac gctcccacaa aatgcggcca aatttgccaa agcgtttaag cagaatcacc
    19021 agatcacact tttgcacatc accgcaggtc cgcagcccca tcgcttccag ttttgccgcc
    19081 gagactttgc cgacgccggg aatttttgcc agcggtaagg tttgcagaaa tgccggaact
    19141 tctgccggcg taatcacaaa ctgaccgttg ggtttattca tgtcggaggc gattttggcg
    19201 agaaacttta ctggtgccac gcccgcagat gccgtcagtt gcagctcgtt gaagatcgtc
    19261 tggcggattt cctgggcgat gagggtcgca gaaccgtggc aatggacgct atcagtgaca
    19321 tcgagatagg cttcatccag tgacaacggt tcaatacgcg aggtgtagcg cgagaagatt
    19381 tcacggatat gatttgaggc ttctttgtag gcgtcaaagc gccccggaag caaggtgaga
    19441 tgcgggcata atttgagcgc catccctgtc ggcatagcgc tacgtacgcc aaatttacgc
    19501 gcgggataat tggcggtgct gattaccccc cgtcgttcgc ggctgccgcc aatagcaata
    19561 gggatatcgc gcagggcggg attgtcgcgc atctccaccg ctgcgaaaaa gcagtccata
    19621 tccacatgaa tgattttacg cattgctcac ctctcaacac tggtaaagta tacagtgatt
    19681 tcagggtttg agaaatgcgt aaagattcag catttaacgc gctcgctgcg aaagcacctg
    19741 ctgcttatcc agcttttgca ccagcggctg caccacttta tccccatgct gaccaaagta
    19801 ttgatacagc gtatctgacg cacttttggt cagcaccata atctcaatgc gccggttgcc
    19861 cgcgctttgc ggatttttgg catccagcag catctggtcc gccattgcgc ttacctgcat
    19921 cactttattt tccggcattc cggcctcttc cagcacccga cgagccgaaa gcgcgcgatc
    19981 gcccgaaagg ttccagttgt tgtagatatt gtttttgtag gccatcgcat cggtatgccc
    20041 ggtaataata attttgttat cgagcgagtc gaacactggc gcgagctcca ccagcagcgt
    20101 tttaaagaac ggcataatct tcgcgctgcc gcgttcaaac atattgcggt tctggtcatc
    20161 tttaatcagc acgcgcagtc cctggggaac aatctccatc tccagattcg cttccatatg
    20221 tgcatcacgg gcaatggtgt tgatactggt cgccagctcg ccaagctcgg tggcggattt
    20281 cttcttcagc agcgccgttt tctcgttgac gtcgcgcgct tttttctccg tctcttccgg
    20341 caccgccacg gtggcaggtt ttggcggatg agacgggctg attttattta acggcgacaa
    20401 cccaccaccg ttaaaaatcg actgcccgtg cagcgcggcg ataatgcttt cacgctcgga
    20461 tttactgacg ctattgacaa tcaactttat cgtcgtcacc aaaggggccg agcgtatttc
    20521 cgaggtttcc gcccgcttta ccttagacgc gatgcccggt aagcagatgg cgattgacgc
    20581 cgatcttaac gccgggttga tcaaccaggc gcaggcgcaa acccggcgta aagatgttgc
    20641 cagcgaggcc gatttctacg gcgcaatgga cggggcatcg aagtttgtgc gcggggacgc
    20701 catcgccggg atgatgattc tggcgatcaa cctgatcggc ggcgtctgta tcgggatctt
    20761 caaatacaac ctgagcgccg acgccgcctt ccagcaatat gtgctgatga ccatcggcga
    20821 cgggctggtg gcgcagatcc cttccctgct gctctccacc gcggcggcga ttatcgtcac
    20881 ccgcgtcagc gacaacggcg atatcgccca tgacgtgcgc caccagctgc tggcaagccc
    20941 gtcggtgctc tacaccgcca ccggaattat gtttgtactg gcggtggtgc cgggaatgcc
    21001 gcacctgccg tttttgctgt tcagcgccct gcttggcttt accggctggc ggatgagcaa
    21061 acgcccgcag gcggcggagg cggaggagaa aagtcttgaa acgctaaccc gcaccatcac
    21121 tgaaaccagc gagcaacagg tcagttggga aaccattccg ctgatcgagc ctatcagctt
    21181 aagcctcggc tacaagctgg tggcgctggt cgataaagcg caggggaacc cactcaccca
    21241 gcggattcgc ggcgtgcggc aggtgatatc cgacggcaac ggcgtgctgc tgccggagat
    21301 ccgtattcgg gaaaacttcc gcctcaagcc cagccagtac gccatattca tcaacggcat
    21361 taaggccgat gaagcggata ttccggcaga taaactgatg gccctgccct ccagcgaaac
    21421 ctacggcgag attgacggcg tactggggaa tgacccggca tatgggatgc cggtcacctg
    21481 gattcagcct gcgcagaaag cgaaggcgct gaatatgggg tatcaggtga tcgacagcgc
    21541 cagcgtgatt gccacccatg tgaataagat tgtgcgcagc tatattcctg atttatttaa
    21601 ctatgacgat attacgcagt tgcataaccg cctgtcgtcg atggcaccgc gactggcgga
    21661 agacttaagc gcagcgctca attacagcca gttgctgaaa gtgtatcgcg cgctgctgac
    21721 cgaaggcgtt tccctgcgcg atattgtcac catcgccacc gtgctggtcg ccagtagtgc
    21781 ggtgactaaa gatcatatcc tgctggcggc agatgtgcgc ctggcgctgc ggcgcagtat
    21841 cacccatccg ttcgttcgca agcaggagct gacggtgtat acgctgaata atgagctgga
    21901 aaatctgctg accaacgtgg tgaatcaggc gcaacaggcc ggcaaagtga tgctcgacag
    21961 cgtgccggtg gacccgaata tgcttaacca gttccagagt acgatgccac aggtgaagga
    22021 gcagatgaaa gcggcgggga aagacccggt gctgctggtg ccgccgcaac tgcgtccatt
    22081 gctggcgcgc tatgcaaggt tgtttgcgcc ggggctgcat gtgctgtcgt ataacgaagt
    22141 gccggatgag ctggagttga agattatggg ggcgttgatg taagaatccg gcatcggtgc
    22201 acaattgccg gatgcggcgt gaacgcctta tccggcctac cgttaaggca tagatttgta
    22261 ggcatgataa gacgcgctag cgtcgcatca ggcatctgcg cacaaccgcc ggatgcggcg
    22321 tgaacgcctt atccggccta ccgttaaggc acagatttgt aggccggata agcgtaagcg
    22381 catccggcac cggcgcacaa ttataaaata atacgctccc cggcactaaa atccgttacg
    22441 tcccccgccc aatcgatggg atataaacct ttcgcgacat cgcgatggaa cgttgagaat
    22501 ggccaatcac tcacctgctt tacccagcca tgtttcactg gatttatgta aatataatca
    22561 acatgatgac gataatcttt ggtattgcga atggcatgct cccaaaaacg cggttgccac
    22621 atattttgca atccacaagc atgggtgaac tgttttttaa tctcccgcca gcgagaggaa
    22681 aaatcatcat cgctttcggg taatgtccag atacagtgca tatgttccgg caaaacgacc
    22741 caggcgttaa tttcaaaagg tctggcctgc ttaactttaa taatggcgtt acggagtgtc
    22801 tggtactggg tggtcaaaag ttggcttcga cgattccgta aatttaccgt gaaaaaccat
    22861 gttcccccct tgatgtaata acgacgatat tcagacatgg cgactccttt ttctcaacga
    22921 taagaagaaa aggaaaagtc gacaatgaag ttgtgttata tcgggaaagc agctcgcaaa
    22981 aaattcgcag actcgtaggc atgataagac gcggcagcgt cgcatcaggc atcggtgcac
    23041 tattgccgga tgcggcgtga acgccttatc cgtcctacaa aatcgtttcc tccgtcaaaa
    23101 tcctgcgcgc gcctaaaaaa tggtcctgcc agaaaggttc ggctaaccga cttacccgaa
    23161 tggtttcccc ggtgcgcggt gactcgataa actgaccgtc tccaagatat acgcccatat
    23221 ggtcggctat ctcacgactg tggatatgga aaaacagcaa atctccccgg cgcaggtcgt
    23281 tattcgccac aatcgttgcc cgatggtagt gatacatctc attggccgta cgcgggagct
    23341 tcgcctcgag gatcttgttg taggcataaa aaaccaaccc gctacagtcg aagccttgat
    23401 cgggccgcgt gccgccccag acgtaaggct taccaagctg ctgctccagg cggtgaatcg
    23461 ccacttcggt gatgttgtgt agatggtcgc tgcttaagaa atggttgttc tccgccagcg
    23521 cctgccagcg tctgtcactc gccttgagcg gcgcaggaaa ccattctgga tgctgtttta
    23581 tctgctcccg attatgctgg cgcagcgccc ttctgctttt ggcattgcct tccacaatat
    23641 agctggcctg ctttttcaga tgggtttggt actgttttaa taaacgcgca cggttttgca
    23701 tccgctggcg ggcggcgtag ctgaaagaga tatgtgatgt ggtggcgctg gcgagtaaag
    23761 ggagcaaaac gcataacgaa aacacgcctg gatgcagaaa tctgccgtgc aagaacgaag
    23821 acatgaagga cataaaccag ctgaatgaag gtgagaattt taaaagaccg gaggagtaaa
    23881 cgggattatt aattgcttat ttgcatatta ctattgcggt ttcataatct taggaaataa
    23941 gaaataaatc tacaatccat gcgcttttcg gactcttgaa aactgcccct gtgctaaatt
    24001 tgtaaaccct gcgccgacag gcttgcgtaa aactcttttg gtgataatgt gagccttcca
    24061 aatccagact attccgattt gggattcatt ttgccgtcct ggcgtgtgta accgttttat
    24121 caaggaataa gcagtatgcg taaaatcgca ttaattcttg cgatgttgtt gatcccgtgc
    24181 gtttcgtttg ccggtttgct gggcagcagt agctcgacca cgccagtgag caaagagtat
    24241 aagcagcagt tgatgggatc ccctgtctac atccagatct tcaaggaaga acgtacgctc
    24301 gatctctacg tcaaaatggg cgagcaatat caactgctcg acagctataa aatctgtaaa
    24361 tattccggcg gcttagggcc aaaacagcgt cagggcgatt tcaaaagccc ggaagggttt
    24421 tatagcgtcc agcgtaatca gttaaaacca gacagccgtt actacaaagc gattaatatt
    24481 ggattcccaa acgcctatga ccgtgcgcat ggttatgaag ggaaatacct gatgattcac
    24541 ggcgattgtg tttccatcgg ctgctacgcg atgaccaatc agggtattga tgagatcttc
    24601 cagttcgtta ctggtgcgct ggtgtttggt cagccgagcg tgcaagtgag tatttacccg
    24661 ttccgtatga ccgacgccaa tatgaagcgc cataaatatt ccaactttaa ggacttctgg
    24721 gagcaactga agccaggcta cgactacttt gagcaaaccc gtaagccacc gaccgtttct
    24781 gtggtcaatg gccgttacgt ggtcagcaag ccgttaagcc acgaagttgt gcagccacaa
    24841 ctggcatcaa actacacgct ccccgaggca aaataagcgc cattcacctg gcataatctt
    24901 ttgccaggtt tcattgcccg tcagcggctg tgtcgcaatc accgtgacca catcattcgg
    24961 tgtggtctgc gagctgaagt cgatttccac atcctgatcc agcaacgttg ccacgccaaa
    25021 cggcgcgcgg cgggtgatcc agtgtaaatt agtcgagcaa tacgccatta catagcgccc
    25081 gtcagaaagc agcatgttga aaacgccctt ctgccgcagt tcatccgcca gtgaggcgat
    25141 atatttaaac actgccgcca tgttgcccgg tgtgcgcggg taacgctgcg ttaatttatg
    25201 caggagccag caaaaggctt tttcgctatc ggtttcaccg accgggcgga agttgccggt
    25261 ttccagtgat ttgtagcccg tcagttgtcc attatgggca taagtccagt tacgccccca
    25321 taactcgcgg gtaaacgggt gagtattttc cagcgccacc tcgccccgat tagcctggcg
    25381 aatatgagcc accaccgaac aggattttat cgggtagtcc tggacaagtt tggcgatggg
    25441 ggaattaaag ctgggttgcg gatctttaaa tgtgcgacag cctttacctt cgtaaaaggt
    25501 aatgccccag ccatctttat gtggcccggt tccaccacca cgctgtacaa gtccggtgaa
    25561 actaaagcag atatcggtag ggacgttggc gctcatcccg agcaattcgc acatgggtaa
    25621 cctccacaac agcgggagcg catggccccc ggcgaagatt gattaaaaag tacaaaggtt
    25681 caggctgtta ccctcacccc ggccctctcc ctgaaaggga gaaaagacct tacttaacca
    25741 tctctttttc aattaactgg atcaggatat ggatcacttt aatgtggatc tcctgaatgc
    25801 ggtcggcata accaaagtgc ggtacgcgaa tttcgatatc cgctgtgcca gccattttgc
    25861 cgccgtcttt accggtcagg gtgatcactt tcatcccctt ctcacgcgcc gccgcgatcg
    25921 ctttgatcac gtttgcagag ttaccggagg tggagatccc cagcagtaca tcgccttcgc
    25981 gacctaccgc ttcaacgtaa cgggagaaaa tatcattgaa accaaaatcg ttaccgacgc
    26041 aggaaatatg gctgacgtca gaaatggcaa tcgccgggta gcccggacgg ttttcacggt
    26101 agcgaccggt caactcttcg gcaaagtgca tagcgtcgca atgggagccg ccgttgccgc
    26161 aggaaagcac tttgccaccg gctttaaagc tgtctgctaa caggaccgcc gcgcgctgaa
    26221 tggcgtgaat attggcgtca tcttttaaaa aattagccag cgtttccgcc gcttcgttca
    26281 gttcgttacg aataagatcc tggtacatga ggatatcctt cagcataaat gtaatagaca
    26341 aaatgcagtg taccggatac cgccaaaagc gagaagtacg ggcaggtgct atgaccagga
    26401 ctttttgacc tgaagtgcgg ataaaaacag caacaatgtg agctttgttg taattatatt
    26461 gtaaacgtat tgctaaatgt ttttacatcc actacaacca tatcatcaca agtggtcaga
    26521 cctcctacaa gtaaggggct tttcgttatg atgattttga gtattctcgc tacggttgtc
    26581 ctgctcggcg cgttgttcta tcaccgcgtg agcttattta tcagcagtct gattttgctc
    26641 gcctggacag ccgcactcgg cgttgctggt ctgtggtcgg cgtgggtact ggtgcctctg
    26701 gccattatcc tcgtgccatt taactttgcg cctatgcgta agtcgatgat ttccgcgccg
    26761 gtatttcgcg gtttccgtaa ggtgatgccg ccgatgtcgc gcactgagaa agaagcgatt
    26821 gatgcgggca ccacctggtg ggaaggcgat ttgttccagg gcaagccgga ctggaaaaag
    26881 ctgcataact atccgcagcc gcgcctgacc gcagaagagc aagcgtttct cgacggcccg
    26941 gtagaagaag cctgcaggat ggcgaatgat ttccagatta cccatgagct ggcggatttg
    27001 ccgccggagc tgtgggcgta tcttaaagag catcgcttct tcgcgatgat catcaaaaaa
    27061 gagtacggcg ggctggagtt ctcggcttat gcccagtctc gcgtgctgca aaaactctcc
    27121 ggagtgagcg ggatcctggc gattaccgtc ggcgtgccaa actcattagg cccgggtgag
    27181 ctactgcaac attacggcac tgacgagcag aaagatcact atctgccgcg tctggcgcgt
    27241 ggtcaggaga tcccttgctt tgcactgacc agccccgaag cgggttccga tgcgggcgcg
    27301 attccggata ccgggattgt ttgcatgggc gagtggcagg gccagcaggt gctggggatg
    27361 cgactgacct ggaacaaacg ctacattacg ctcgcgccga ttgcgaccgt gcttggtctg
    27421 gcgtttaaac tctccgaccc ggaaaaactg ctgggtggcg cagaagattt aggtattacc
    27481 tgtgcgctga tcccgaccac cacgccgggc gtggaaattg gtcgtcgcca cttcccgctg
    27541 aacgtgccgt tccagaacgg accgacgcgc ggtaaagatg tcttcgtacc gatcgattac
    27601 attatcggcg gacccaaaat ggccgggcaa ggctggcgga tgctggtgga gtgcctctcg
    27661 gtaggccgcg gtatcaccct gccttccaac tcaaccggcg gcgtgaaatc ggtagcgctg
    27721 gcaacgggtg cgtatgcgca catccgccgt cagttcaaaa tctctattgg taagatggaa
    27781 gggattgaag agccgctggc gcgtattgcc ggaaacgctt atgtaatgga tgctgcggca
    27841 tcgctgatta cctacggcat tatgctcggt gaaaaacctg ccgtgctgtc ggctatcgtt
    27901 aagtatcact gtacccaccg tgggcagcag tcgattattg atgcgatgga tatcactggt
    27961 ggcaaaggca ttatgctcgg gcaaagtaac ttcctggccc gtgcttacca gggcgcaccg
    28021 attgccatca ccgttgaagg ggctaacatt ctgacccgca gcatgatgat cttcggacaa
    28081 ggagcgattc gttgccatcc gtacgtgctg gaagagatgg aagcggcgaa gaacaatgac
    28141 gtaaatgcgt tcgataaact gctgttcaaa catatcggtc acgtcggtag caacaaagtt
    28201 cgcagcttct ggctgggcct gacgcgcggt ttaaccagca gcacgccaac cggcgatgcc
    28261 actaaacgct actatcagca cctgaaccgc ctgagcgcca acctcgccct gctttctgat
    28321 gtctcgatgg cggtgctggg cggcagcctg aaacgtcgtg agcgtatctc ggcacgtctg
    28381 ggggatattt taagtcagct ttacctcgcc tctgccgttt tgaagcgtta tgacgacgaa
    28441 ggccgcaacg aagccgacct gccgctggtg cactggggcg tacaggatgc gctgtatcag
    28501 gcagaacagg cgatggacga tttactgcaa aacttcccga accgcgtggt tgccgggctg
    28561 ctgaatgtgg tgatcttccc gaccggacgt cattatctgg caccttctga caagctggat
    28621 cataaggtgg cgaagatttt acaagtgccg aacgccaccc gttcccgcat tggtcgcggt
    28681 cagtacctga cgccgagcga gcataacccg gttggtttac tggaagaggc gctggtggat
    28741 gtgattgccg ccgacccgat tcatcagcgg atctgtaaag agctgggtaa aaacctgccg
    28801 tttacccgtc tggatgaact ggcgcacaac gcgctggcga aggggctgat tgataaagat
    28861 gaagccgcta ttctggtgaa agctgaagaa agccgtctgc gcagtattaa cgttgatgac
    28921 tttgatccgg aagagctggc gacgaagccg gtaaagttgc cggagaaagt gcggaaagtt
    28981 gaagccgcgt aattgaataa cggagccgaa aggctccgtt tctttatccg ctaattattt
    29041 aaaattaaag ccatccggat ggttttccag gctgccggtc aacgccgcga acagcaccgt
    29101 tttaccatca atagaaagcg catcgttcac attcaaccag gtgagtttct cttgcgacgt
    29161 tttctcatca atagtcgaga acagccccgt catctgatta gatttctcgg accacatcac
    29221 agcgatacgt tgcgagccac agtcatgcgg tttgcacgcg ctcatcacct gatacgtctc
    29281 atctcccaat gtcacggttt gtgcgggagt ataagtaccg cctttcatca cccaggcagg
    29341 cagcttatgc ccttgcacca tctgattaaa cgccgctttg gtggtttcgc cctttgcaag
    29401 actgctaatg gttaaatcat cctgcgccat tgcactggtg gcgatgacca gagcggcgac
    29461 tgtcgttatt gccttaaaca tcattcctcc cgggcttatc ctgcccatgt gattaactcc
    29521 ctaaagcctg gcgataacac gataatttgc aaatcagata tttccaatat cagcatttgt
    29581 tatcaattta ttctgcgcta aggcaactgc ctgataaggt gttagagtta taaaaccctt
    29641 cgcataaagg agttgtacac cgtgcctggt ttgaagatta cgcttttgca gcaaccactg
    29701 gtgtggatgg atggtcctgc caacctgcgc cattttgatc gtcaactgga aggtattacc
    29761 gggcgcgatg tgatcgttct accggagatg tttaccagcg gctttgccat ggaagcggca
    29821 gcttcgtcgc tagcacaaga tgacgtagtg aactggatga ccgctaaagc gcagcagtgc
    29881 aatgcgctga ttgcaggcag tgttgcatta caaacggagt ctggttcggt taaccgcttt
    29941 ttgctggttg agccgggcgg cacggtacat ttttatgata agcgtcatct gttccgcatg
    30001 gcagatgagc atctgcatta taaagcgggc aatgagcgag tgattgtgga atggcgcggc
    30061 tggcgtattt tgccgctggt gtgctacgac ttacgttttc cggtgtggtc gcgcaatctc
    30121 aacgattatg acctcgccct gtacgtcgcc aactggcctg ctccgcgctc tctgcactgg
    30181 caggcattgc tgacagcccg cgcgattgag aatcaagcgt atgtggcggg atgcaatcgc
    30241 gtcggcagcg atggcaacgg ctgccattat cgcggtgaca gccgggtgat taatccgcaa
    30301 ggcgagatta tcgctactgc cgacgcgcat caggcaacgc gcattgatgc ggagctgtcg
    30361 atgatggcat tgcgggaata tagagaaaag tttccggcat ggcgggatgc ggatgagttt
    30421 aggttgtggt gagtgggggt tgtgtttagg gctggggaga gtcagggtaa gactacgaaa
    30481 gcccgctc
//
LOCUS       DAZGQL010000066        30027 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.066, whole genome shotgun sequence.
ACCESSION   DAZGQL010000066 DAZGQL010000000
VERSION     DAZGQL010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30027
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.066"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(163..238)
                     /locus_tag="ACHKIR_003200"
     tRNA            complement(163..238)
                     /locus_tag="ACHKIR_003200"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(203..205),aa:Gly,seq:gcc)
     gene            complement(449..994)
                     /gene="orn"
                     /locus_tag="ACHKIR_003201"
     CDS             complement(449..994)
                     /gene="orn"
                     /locus_tag="ACHKIR_003201"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFO9990889.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            1089..2141
                     /gene="rsgA"
                     /locus_tag="ACHKIR_003202"
     CDS             1089..2141
                     /gene="rsgA"
                     /locus_tag="ACHKIR_003202"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HFO9990890.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            2238..3206
                     /gene="asd"
                     /locus_tag="ACHKIR_003203"
     CDS             2238..3206
                     /gene="asd"
                     /locus_tag="ACHKIR_003203"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HFO9990891.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDANPLVDDNKDQV"
     gene            3228..6551
                     /gene="mscM"
                     /locus_tag="ACHKIR_003204"
     CDS             3228..6551
                     /gene="mscM"
                     /locus_tag="ACHKIR_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HFO9990892.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQISQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQEVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNSPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLLGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(6582..6896)
                     /locus_tag="ACHKIR_003205"
     CDS             complement(6582..6896)
                     /locus_tag="ACHKIR_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeO family protein"
                     /protein_id="HFO9990893.1"
                     /translation="MSARMFILCCIWFIVAFLWITITSALDKEWMIDGRGINNVCDVL
                     MYLEDDDTRDVGVIMTLPLFFPFLWFALWRKKRGWFMYATALAIFGYWLWQFFLRYQF
                     CL"
     gene            complement(6893..7207)
                     /gene="yjeN"
                     /locus_tag="ACHKIR_003206"
     CDS             complement(6893..7207)
                     /gene="yjeN"
                     /locus_tag="ACHKIR_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjeN"
                     /protein_id="HFO9990894.1"
                     /translation="MDDASRDPVITEDEIRELQFSAGDVAEIEQTVLSFVDTRHTRKV
                     AMVVGNTINTLKERDGPRWGNLPDIYCAYLIRCLVFRGELVGYGDLFRMRYSEIKRPI
                     IS"
     gene            complement(7270..8772)
                     /gene="yjeM"
                     /locus_tag="ACHKIR_003207"
     CDS             complement(7270..8772)
                     /gene="yjeM"
                     /locus_tag="ACHKIR_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HFO9990895.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTHMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(8991..9968)
                     /gene="epmA"
                     /locus_tag="ACHKIR_003208"
     CDS             complement(8991..9968)
                     /gene="epmA"
                     /locus_tag="ACHKIR_003208"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFO9990896.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            10293..12101
                     /gene="frdA"
                     /locus_tag="ACHKIR_003209"
     CDS             10293..12101
                     /gene="frdA"
                     /locus_tag="ACHKIR_003209"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFO9990897.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAVIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            12094..12828
                     /gene="frdB"
                     /locus_tag="ACHKIR_003210"
     CDS             12094..12828
                     /gene="frdB"
                     /locus_tag="ACHKIR_003210"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFO9990898.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            12839..13234
                     /gene="frdC"
                     /locus_tag="ACHKIR_003211"
     CDS             12839..13234
                     /gene="frdC"
                     /locus_tag="ACHKIR_003211"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFO9990899.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            13245..13604
                     /gene="frdD"
                     /locus_tag="ACHKIR_003212"
     CDS             13245..13604
                     /gene="frdD"
                     /locus_tag="ACHKIR_003212"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFO9990900.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            13667..14800
                     /locus_tag="ACHKIR_003213"
     CDS             13667..14800
                     /locus_tag="ACHKIR_003213"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFO9990901.1"
                     /translation="MFKMTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGI
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDDVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANASIGLFGALAVKPSGLSFEQAMQTRVFQPLKLTHTWINVPSAEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLKPLDITEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDIIINGSDNKIALAARPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            14889..15422
                     /gene="blc"
                     /locus_tag="ACHKIR_003214"
     CDS             14889..15422
                     /gene="blc"
                     /locus_tag="ACHKIR_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFO9990902.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(15423..15740)
                     /gene="sugE"
                     /locus_tag="ACHKIR_003215"
     CDS             complement(15423..15740)
                     /gene="sugE"
                     /locus_tag="ACHKIR_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFO9990903.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(15915..16061)
                     /gene="ecnB"
                     /locus_tag="ACHKIR_003216"
     CDS             complement(15915..16061)
                     /gene="ecnB"
                     /locus_tag="ACHKIR_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFO9990904.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(16172..16297)
                     /gene="ecnA"
                     /locus_tag="ACHKIR_003217"
     CDS             complement(16172..16297)
                     /gene="ecnA"
                     /locus_tag="ACHKIR_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFO9990905.1"
                     /translation="MMKRLIVLVLLASALLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(16349..16915)
                     /gene="efp"
                     /locus_tag="ACHKIR_003218"
     CDS             complement(16349..16915)
                     /gene="efp"
                     /locus_tag="ACHKIR_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFO9990906.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            16957..17985
                     /gene="epmB"
                     /locus_tag="ACHKIR_003219"
     CDS             16957..17985
                     /gene="epmB"
                     /locus_tag="ACHKIR_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFO9990907.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPNDPLLRQVLTSQDEFVVAPGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITDALVERFSHSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            18220..19089
                     /locus_tag="ACHKIR_003220"
     CDS             18220..19089
                     /locus_tag="ACHKIR_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HFO9990908.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFLSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMVENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVINELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQDNGNLEYDTYSQPEWKHNLFNHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEVEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(19139..19492)
                     /locus_tag="ACHKIR_003221"
     CDS             complement(19139..19492)
                     /locus_tag="ACHKIR_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HFO9990909.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(19630..21276)
                     /gene="groL"
                     /locus_tag="ACHKIR_003222"
     CDS             complement(19630..21276)
                     /gene="groL"
                     /locus_tag="ACHKIR_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFO9990910.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(21320..21613)
                     /locus_tag="ACHKIR_003223"
     CDS             complement(21320..21613)
                     /locus_tag="ACHKIR_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFO9990911.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            21889..23145
                     /gene="yjeH"
                     /locus_tag="ACHKIR_003224"
     CDS             21889..23145
                     /gene="yjeH"
                     /locus_tag="ACHKIR_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HFO9990912.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEKMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLSRLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLTMVGWKS
                     LYALIMLAGLWLFLPKRKTPENGITT"
     gene            complement(23161..23637)
                     /locus_tag="ACHKIR_003225"
     CDS             complement(23161..23637)
                     /locus_tag="ACHKIR_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HFO9990913.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            23974..25410
                     /gene="aspA"
                     /locus_tag="ACHKIR_003226"
     CDS             23974..25410
                     /gene="aspA"
                     /locus_tag="ACHKIR_003226"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HFO9990914.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            25529..26830
                     /gene="dcuA"
                     /locus_tag="ACHKIR_003227"
     CDS             25529..26830
                     /gene="dcuA"
                     /locus_tag="ACHKIR_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HFO9990915.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            26946..27284
                     /gene="cutA"
                     /locus_tag="ACHKIR_003228"
     CDS             26946..27284
                     /gene="cutA"
                     /locus_tag="ACHKIR_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HFO9990916.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            27260..28957
                     /gene="dsbD"
                     /locus_tag="ACHKIR_003229"
     CDS             27260..28957
                     /gene="dsbD"
                     /locus_tag="ACHKIR_003229"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HFO9990917.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIANVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVPQQEQPT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDIWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            28994..29569
                     /locus_tag="ACHKIR_003230"
     CDS             28994..29569
                     /locus_tag="ACHKIR_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO9990918.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDAWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            29676..29751
                     /locus_tag="ACHKIR_003231"
     tRNA            29676..29751
                     /locus_tag="ACHKIR_003231"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29709..29711,aa:Phe,seq:gaa)
BASE COUNT         7363 a         7856 c         7567 g         7241 t
ORIGIN      
        1 cccgcttaat cttcgtcttt cgaaccgcta ctgccttggc tgccttcact caccttagtc
       61 acttactttt gtaagttccc aaggattcgt tcagttgccg ccttgtcgca attcgaaata
      121 ctgcgattgg gtggtctgtg ctttgcagca cttttcaaat tttggagcgg gaaacgagac
      181 tcgaactcgc gaccccgacc ttggcaaggt cgtgctctac caactgagct attcccgcgt
      241 actacttaat tttgctttcg taattttcgc atttctgcgt cgttacggga ggcgcattat
      301 acgagaaatc tttccctctg caacccccct aaaaaatttt ttttgagttt ttatctcaag
      361 tgctgaaatt atcagcaaga cgttcaattt agcgtcaaaa tcacgccgcc gcaagtggat
      421 tccggcgctt acgtgaccag gaaaaatttt acagcttgat aaaatgctcg cggtagtaag
      481 ccagttctgc caccgactca cggatatcat ccatcgcctg atgcgtcccc tgcttggtaa
      541 aaccatcaag aatttccggc ttccagcggc gcgccagctc tttcagggtg ctgacatcga
      601 gataacggta gtggaagtag gcttccagct ccggcatata tttaaacagg aagcgacgat
      661 cctggccgat gctgttaccg caaatcggcg attttcccgc aggcacccac tgttttaaga
      721 attcgagcgt tgccagttca gcttcacgat cgcccatcgt gctcgctttc acgcgctcca
      781 ccagcccgct ggcggtatgg gtgcgcacgt tccagtcatc catcagcgcc agctgttcat
      841 cagactggtg tactgcaatg gtcggccctt ctgccagaat attcaggttg gcatcggtca
      901 ccagcgtggc aatctcaata atgcgatcgc gctcgggatc cagaccggtc atctcaagat
      961 cgatccaaat caggttgttt tcattggcac tcatgctatt ttccacccat tcacagtgac
     1021 tatattgatc cacaattgcg tgtatcatag atgttttgcc catcaggggc gaccaggagt
     1081 ctgtacgatt gagtaaaaat aaactctcca aaggccagca gcgccgcgtg aacgccaatc
     1141 accagcgtcg tcttaaaacg tctaaggaga agcccgacta cgacgacaat ctgtttggcg
     1201 agcctgatga aggtatcgtc atcagccgct ttggtatgca cgctgatgtg gaatccgccg
     1261 atggcgacgt tcaccgctgc aatattcgcc gtaccatccg ttcgttggtg accggcgacc
     1321 gcgtagtctg gcgtccgggt aaaccagcgg cggaaggcgt aaatgtcaaa gggatcgtgg
     1381 aagcggtgca tgagcgcacc tcagtgttga cgcgcccgga tttttacgac ggtgtgaaac
     1441 ctattgctgc caacatcgac cagattgtca ttgtctccgc cattttgccg gagctgtcgc
     1501 tcaatattat cgaccgttac ctggtggcct gcgaaacctt gcagattgag ccgattattg
     1561 tgctcaacaa gatagacctg ctggacgacg aaggcatggc attcgtcaac gagcagatgg
     1621 atatctaccg caatatcggt tatcgcgtat tgatggtttc cagtcatact caggatgggc
     1681 tgaaaccgct ggaagaggcg ttgaccgggc gcatcagcat ttttgccggg cagtctggcg
     1741 ttggcaaatc cagcctgctg aacgcattgc tgggtctgca aaaagagatc ctgaccaacg
     1801 atgtctctga caactcgggt ctcggtcagc acaccacgac cgccgctcgg ctgtatcact
     1861 tcccgcacgg cggtgatgtg attgactccc caggagtgcg tgagttcggc ctctggcacc
     1921 tggagccgga acaaatcact cagggctttg tcgaattcca tgactactta ggtctgtgta
     1981 aatatcgcga ttgcaaacac gataccgatc cgggctgcgc tatccgggaa gcggttgagg
     2041 aaggaaaaat cgcggaaacc cgtttcgaaa actatcaccg tattctggaa agcatggcgc
     2101 aggtaaaaac gcgtaaaaac ttttctgata cggatgactg acaattaagc taaccgtcgt
     2161 tagaatcgtc cccttttttt caggatccgg catgtaggcc ggatcaggaa cgacaaaaca
     2221 atggcctgga ggctaccttg ttaaattcat ttaaactttc gctacagtac attctgccga
     2281 aactatggct tactcgcctg gcgggttggg gcgcaagcaa gcgggcagga tggctgacaa
     2341 aactggttat cgatctgttc gttaaatact acaaggtcga tatgaaagag gcgcaaaagc
     2401 cggacaccgc cagctaccgc acctttaacg aattctttgt ccgtccgctg cgtgacgaag
     2461 tacgcccaat cgataccgat ccgaatgtac tggttatgcc tgccgatggc gttatcagcc
     2521 agttgggtaa aatcgaagaa gataaaatcc tgcaagccaa aggccacaac tacagcctcg
     2581 aagccctgct ggctggcaac tatctgatgg cggacctgtt ccgcaacggt acgtttgtga
     2641 ccacttacct ctccccacgt gactaccacc gcgtacacat gccgtgcaac ggtattctgc
     2701 gtgagatgat ctacgtgccg ggcgatctct tttccgttaa ccatctcacc gctcagaacg
     2761 tgccgaatct gtttgcccgt aacgaacgcg tgatctgcct gttcgatacc gaatttggcc
     2821 caatggcgca gattctggtc ggggcgacga ttgtaggcag cattgagacg gtctgggcgg
     2881 gcaccattac gccgccgcgc gaaggtatca tcaagcgctg gacctggcct gccggggaaa
     2941 acgacggttc tgtggcactg ctgaaaggcc aggaaatggg tcgctttaaa ctcggttcca
     3001 ccgttatcaa cctgtttgca ccgggtaaag tgaatctggt tgagcaactg gaaagcctgt
     3061 ctgtcacgaa aattggccag ccgctggcag tatctaccga aacctttgtt acaccagacg
     3121 ctgaaccggc cccgcttcct gctgaagaga tcgaagcaga acacgacgcc aacccattgg
     3181 ttgacgacaa caaagaccag gtctaatcca tcaaaggaaa cgctgacgtg cgcctgatta
     3241 tcacttttct gatggcctgg tgcctcagtt ggggggcgta cgccgcgacg gcccccgata
     3301 gcaaacaaat ctcacaagaa ctggagcagg caaaagcggc gaaacccgcg cagccggaag
     3361 tcgtagaggc gctccagtcc gccttaaatg cgcttgagga acgaaaaggt tcccttgagc
     3421 gcatcaagca atatcaagaa gtcattgata attacccgaa actttccgct actctgcgcg
     3481 cacaattaaa caacatgcgt gacgaaccgc gcagcgtgtc accgggaatg tctaccgacg
     3541 cgctgaatca ggaaattctc caggtcagca gccagttgct ggataaaagc cgtcaggccc
     3601 agcaagagca ggaacgcgcc cgcgagattg ccgattcgct gaatcaactg ccgcaacagc
     3661 aaaccgatgc ccgccgtcag ttaaatgaaa tcgagcgccg cctggggacg cttaccggca
     3721 attccccgct caatcaggca caaaatttcg cgttgcagtc tgactccgca cgcctgaaag
     3781 cgctcgtcga tgaactggaa ctggcgcagc tgtctgccaa taaccgtcag gaattagcgc
     3841 gcttacgctc agagctggcg gaaaaagaga gccagcaact ggatgcgtat ttgcaggcct
     3901 tacgtaatca gttgaacagc caacgtcagc ttgaggcgga gcgggcgctg gaaagtaccg
     3961 aattactggc agaaaacagt gccgatttgc cgaaagatat cgtcgcgcaa ttcaaaatta
     4021 accgcgaact atcggcagcg ctgaatcaac aggcgcagcg gatggatctc gttgcctcgc
     4081 aacagcgtca ggctgccagc cagacgttac aggtccggca ggcgttgaat acgctgcgtg
     4141 aacagtcgca atggctgggc tcgtccaacc tgctcggcga agcgttgcgg gcgcaggtgg
     4201 cacggctacc ggaaatgcca aaaccacaac agcttgatac cgaaatggcg cagttgcgtg
     4261 tgcaacggtt acgttatgag gatctgctaa ataaacagcc gctgctacgg caaatccatc
     4321 aggccgacgg tcagccgctg accgccgagc aaaaccgtat tctggaagca cagctacgca
     4381 ctcagcgtga gttgctgaac tcattgttgc agggtggaga cacgctacta ctggaactga
     4441 ccaagctgaa agtctccaac gggcaactgg aggatgcgct gaaagaggtg aatgaagcga
     4501 cgcaccgcta tctgttctgg acctctgacg tgcgcccgat gaccatcgcc tggccgctgg
     4561 aaatcgccca ggatctgcgt cgtctcattt cgctggacac cttcagtcag ttgggcaaag
     4621 ccagtgtaat gatgctgacc agtaaagaga cgattttgcc gctgcttggc gcgttgattc
     4681 tggtcggttg cagtatttac tcgcgccgct atttcacccg ttttcttgaa cgttcggcgg
     4741 cgaaagtcgg caaagtgact caggatcact tctggctgac gttacgcact cttttctggt
     4801 cgattctcgt cgcgtcaccg ctgcctgtgc tgtggatgac gctgggttac ggcttgcgcg
     4861 aggcgtggcc ttatccgctg gcggtcgcga ttggtgatgg cgtaacagcc accgtgccgc
     4921 tgctgtgggt agtgatgatt tgcgccacct ttgcccgccc gaacggcttg tttatcgctc
     4981 attttggctg gccgcgcgaa cgggtttccc gtgggatgcg ctactacctg atgagcatcg
     5041 ggcttattgt gccgctgatt atggcgctga tgatgttcga taacctcgac gaccgtgaat
     5101 tctccggttc gctgggacgg ctttgcttta tcctcatttg cggtgcgctg gcggtggtca
     5161 ctctcagtct gaaaaaggcc gggatcccgc tgtatctcaa caaagaaggc agcggcgaca
     5221 acattaccaa ccatatgctg tggaacatga tgattggcgc accgctggtt gccattctgg
     5281 cgtcggcggt gggttatctg gcaacggcgc aggcgctgtt agcgaggctt gaaacctcgg
     5341 ttgccatctg gttcctgcta ctggtggttt atcacgttat ccgccgctgg atgctgatcc
     5401 agcgccgcag gctggcgttt gaccgggcga agcatcgccg ggcagagatg ttagcgcagc
     5461 gtgcgcgtgg cgaagaggaa gcgcatcatc acagtagccc ggaaggagca attgaagtcg
     5521 atgaaagcga agtcgatctc gatgccatca gtgcgcaatc cttgcggctg gtgcgctcaa
     5581 ttttgatgtt gatcgccctg ctttcggtca ttgtgctgtg gtcagaaatc cattccgctt
     5641 tcggcttcct cgaaaatatt tcgctgtggg atgtcacttc cacggtacag ggcgtagaaa
     5701 gcctggagcc gattaccctc ggtgcggtgc tgattgccat tctggtgttt atcatcacca
     5761 cgcagctggt gcgcaacctg cccgcgctgc tggaactggc gattctgcag cacctggatt
     5821 taacgccggg tacgggctac gccatcacca ccatcaccaa atatctgctg atgctgattg
     5881 gcgggctggt cggcttctcg atgattggta ttgagtggtc gaaattgcag tggctggtcg
     5941 ccgcgctcgg tgttggtctc ggttttggtt tgcaggaaat tttcgccaac tttatctctg
     6001 gcctgattat cctgttcgaa aaaccgattc gcattggcga tacggtaacg atccgcgatc
     6061 ttaccggtag tgtgacgaaa attaacaccc gcgccaccac catcagcgac tgggaccgca
     6121 aagaaattat cgtgccgaac aaggcgttta ttaccgagca gtttatcaac tggtcgctct
     6181 ctgactcggt cacgcgcgtg gtgttgacga ttccggcccc tgccgatgcc aacagtgaag
     6241 aagtgacgga aatcctgctc accgcagcgc gtcgctgctc gctggtaatc gataacccag
     6301 caccggaagt cttcctggtg gatctgcaac aggggattca aattttcgag ctgcgtattt
     6361 acgccgctga gatgggtcac cgtatgccac tacgccatga gattcaccag ctaattctgg
     6421 caggattcca tgcccacggt attgatatgc cattcccgcc cttccagatg cgtttggaaa
     6481 gcctgaatgg taagcaaacg gggagaacat taacttctgc gggcaaaggt cgtcaggcgg
     6541 gaagtttgta aggtctttgt ggccggatga acccatccgg cttataagca aaactgatag
     6601 cgcagaaaaa actgccacag ccaatagccg aaaatagcca gcgcggtggc gtacataaac
     6661 cagccgcgtt tttttcgcca caaagcgaac cagagaaaag ggaaaaagag cggtaacgtc
     6721 attatcacac cgacatcacg ggtatcgtca tcttcgagat acatcagcac atcgcagacg
     6781 ttattaatcc cgcgtccatc aatcatccac tctttgtcca gcgcggaagt gatggttatc
     6841 cagaggaacg ccacaataaa ccagatgcag cacaagataa acatgcgcgc actcatgaga
     6901 taatcggtcg cttaatttcg ctatagcgca tacgaaacaa atcgccgtat cccactaatt
     6961 ctccgcgaaa aaccagacat ctaataagat aagcgcaata gatatccggc aggtttcccc
     7021 accgcggacc atcgcgctct ttgagggtat taatagtgtt acccaccacc atcgctactt
     7081 tgcgggtgtg gcgggtatcg acaaaagaga ggaccgtttg ctctatttcc gccacatccc
     7141 cggcagaaaa ttgcagctcc ctgatttcat cttcggttat cactggatcc cgactagcgt
     7201 cgtccataac gtcttccttt ccagacaact catcgccgga gagacgatga gttgcaggat
     7261 ttcatatgat cagtcgagag ctaactcagg tttgttcgcc atgcgactgc aatagttctg
     7321 gtaaatcgcc atcgctaaca gcgagaagaa gacagggccg ccaatcatcc acaatgtgct
     7381 gtcccagtct ccagcttcaa ccacaggttg aatgatggtg aagacgttcg caaatgtcac
     7441 caccagtacg acaaccactg ttgcaatcat tgccgacata tgcgttttga aaatcacaaa
     7501 cggtctgtcg agatcctgac gtgctttaaa gaatgggaaa gccagcgcga ggaacaggta
     7561 aggaagcgtc atagacacgt tcgccatcag cgtcagcttg ttaaagaacg ccgatgcggt
     7621 accgccacca aacgaaacca gcaggatgaa gacagtaacc aacccgcact gcatccacat
     7681 ggcgatagac ggcatcccca tcgcattcag gcgcgtcatc ggttccggcc acaacgcttt
     7741 cggcgtcccc tggatgatgg ctttcagcgg tgaatagcaa agcgtaaaga acgcaccggt
     7801 ataagcgagg aacatcgaaa gtccggtaat acgcgcaaac catacgccca gcgacaatga
     7861 cgcttcaggt gacaaatgca gtgcgttacc cagcgtcacc ccgaggctct tcatcagcac
     7921 ataggtaata ttgccgaggt taacggaacc attacttaat acctgctgcc agtttgtgct
     7981 gacgccccat aaaaatattg ccagcgaata accgattgaa ataacaatag cggcaaaaac
     8041 aatacctttg gcaaagttct tttctggatt ttccgtttta tcgaccagac caccaaccgc
     8101 ttcaattcca ccataggcaa aaatagcaaa taccacaaac gatagcattg ccagaccgga
     8161 ctgataacct gggttcggtg atgcaaggaa attaatatcc tgcgcgaaat gcccaccatt
     8221 taataacaaa atagtaatgc ttactaacag caatactaaa ttcagacaca ttactgcaat
     8281 accgcccacc gcagtaatgc gggcaatttt attaatccct tttgaagcaa cgacggtgac
     8341 cagaatcatc catgccactg ccagcagacc aaccacctgc gtaggctcca gtccggcaat
     8401 acgccagtgc tgggtcatgt cgctaccata gaggaatgtt gagaacggta cccaaacttt
     8461 cgcggaggta ctcaccatcc agatgatata agaggaaaac cacataaacg taccaatgaa
     8521 ggcaaaacgt ggtccgacac tattattcat ccaggaatag ataccgcctt cttctttgcg
     8581 ataagcagct cccatttcag ccatcattaa ggcgaatgga ataaagaata acaatgcaga
     8641 aaatatataa aagggaatcg cactataacc cattaagtaa taagccgatg ggctattggc
     8701 aaatccaaat acggaagtaa agatcatcaa tatgagtcct atcagactca tcttttttat
     8761 cgtgtgaggc atgaaaacat ccttcatttc agcgcgtgta taaaacgcgc ccactgaact
     8821 gcactgccca acattgagcg ggcagcgtaa aaaccaatac agaattgata cctggatgat
     8881 atcaaatatc cagtcttaaa ttgtggcctt tagcggaaaa atgaaaacat atgctacaga
     8941 atggcgctta tcacgccatt cttcgcggtt aattcagtaa tttttcagaa ttatgcccgg
     9001 tcaacgctaa aggcgatgac ttcagccagt gtctccgcgc ccagcgccaa catcaccaga
     9061 cgatcaacgc ctaatgccac gccggaacag tcaggcatac cgactttcaa ggcttcaatc
     9121 agattctggt caatggggtg ctgcggcaaa ccgcgcgccg cgcgcttacg gttatcttgt
     9181 tcaaagcgtt gttgctgctc acgggcatcc gtcaattcat ggaaaccatt cgccagctca
     9241 atacctttat aataaacctc aaagcgttca gcgacccgat gatcttcggt actgatttgc
     9301 gccagtgatg cctggctggc tggaaagtgg tacacaaagg tcggtttttc tttgccaata
     9361 tttggctcta cgccaaaggt aaacagcaat tgtagaagcg tgtcgcggtc ttcttcagta
     9421 tcggcaacgt tgctcaaatc cagtttcgct gccacttccc gcagttgcgt tttgtcggca
     9481 gagagtgggt caatttccag ataacgcaag aaagcttgtt gataagaaag gctttctgct
     9541 gccggacagt ccagcacctg ttgcaagaga tcgtccacct cgttcatcaa ccggtacata
     9601 tcatagtgcg gtcgatacca ctcaagcata gtgaactcag ggttgtgata acgccccatc
     9661 tcttcattac ggaagctgcg gcacagctgg aataccggcc cacaaccagc aaccagcagg
     9721 cgtttcatat ggtattccgg gctggtcatt aaccagagat tcatcccctg cgaatgcccg
     9781 gggcctacga aacgtgtctc aaacgggacc aaatgaatat cggttaccgt cgcctggctc
     9841 atacaaggcg tctccacctc cagcactcca cgatcggcaa agaaacgacg gatctccgcc
     9901 ataatcgccg cgcgttttaa taagttagga atggatgcgc tcggctgcca ggatgccgtt
     9961 tcgctcatag ttaaatctcc agtttttgac aagggcacga agtctactcg caacgcgacg
    10021 gcgagacaaa ttttacgcag gaatcaaaca agggtgggca gtgactaaaa aaagcacgat
    10081 ctgatggttt agtaattaaa ttaatcatct tcagtgataa tttagccctc ttgcgcacta
    10141 aaaaaatcga tctcgtcaaa tttcagactt atccatcaga ctatactgtt gtacctataa
    10201 aggagcagtg gaatagcgtt cgcagaccgt aactttcagg cacttaccct gaagtacggg
    10261 gctgtgggat aaaaacaatc tggaggaatg tcgtgcaaac ctttcaagcc gatcttgcca
    10321 ttgtaggcgc cggtggcgcg ggattacgtg ctgcaattgc tgccgcgcag gcaaatccaa
    10381 atgcaaaaat cgcactaatc tcaaaagtat acccgatgcg tagccatacc gttgctgcag
    10441 aagggggctc cgccgctgtc gcgcaggatc atgacagctt cgaatatcac tttcacgata
    10501 cagtagcggg tggcgactgg ttgtgtgagc aggatgtcgt ggattatttc gtccaccact
    10561 gccccaccga aatgacccaa ctggaactgt ggggatgccc atggagccgt cgcccggatg
    10621 gtagcgtcaa cgtacgtcgc ttcggcggca tgaaaatcga gcgcacctgg ttcgccgccg
    10681 ataagaccgg cttccatatg ctgcacacgc tgttccagac ctctctgcaa ttcccgcaga
    10741 tccagcgttt tgacgaacat ttcgtactgg atattctggt tgatgatggt catgttcgcg
    10801 gcctggtagc aatgaacatg atggaaggca cgctggtgca gatccgtgct aacgcggtcg
    10861 ttatggctac cggcggtgcg ggtcgcgttt atcgttacaa taccaatggc ggcatcgtta
    10921 ccggtgacgg tatgggtatg gcgctaagcc acggcgttcc gctgcgtgac atggaattcg
    10981 ttcagtatca cccaaccggt ctgccaggtt ccggtatcct gatgaccgaa ggctgccgcg
    11041 gtgaaggcgg tattctggtc aacaaaaatg gctaccgtta tctgcaagat tacggcatgg
    11101 gcccggaaac tccgctgggc gagccgaaaa acaaatatat ggaactgggt ccacgcgaca
    11161 aagtttctca ggccttctgg cacgaatggc gtaaaggcaa cactatctcc acgccgcgtg
    11221 gcgatgtggt ttatctcgac ttgcgtcacc tcggcgagaa aaaactgcat gaacgtctgc
    11281 cgttcatctg cgaactggcg aaagcgtacg ttggcgtcga tccggttaaa gaaccgattc
    11341 cggtacgtcc gaccgcacac tacaccatgg gcggtatcga aaccgatcag aactgtgaaa
    11401 cccgcattaa aggtctgttc gccgtgggtg aatgttcctc tgttggtctg cacggtgcaa
    11461 accgtctggg ttctaactcc ctggcggaac tggtggtctt cggccgtctg gccggtgaac
    11521 aagcgacaga gcgtgcagca actgccggta atggcaacga agcggtaatt gaagcgcagg
    11581 cagctggcgt tgaacaacgt ttgaaagatc tggttaacca ggatggcggc gaaaactggg
    11641 cgaagatccg cgacgaaatg ggcctggcta tggaagaagg ctgcggtatc taccgtacgc
    11701 cggaactgat gcagaaaacc atcgacaagc tggcagagct gcaggaacgc ttcaaacgcg
    11761 tgcgcatcac cgacacttcc agcgtgttca acaccgacct gctctacacc attgaactgg
    11821 gccacggtct gaacgttgct gaatgtatgg cgcactccgc aatggcacgt aaagagtccc
    11881 gcggcgcaca ccagcgtctg gacgaaggtt gcaccgagcg tgacgacgtc aacttcctca
    11941 aacacaccct cgccttccgc gatgctgatg gcacgactcg cctggagtac agcgacgtga
    12001 agattactac gctgccgcca gctaaacgcg tttacggtgg cgaagcggat gcagccgata
    12061 aggcggaagc agccaataag aaggagaagg cgaatggctg agatgaaaaa cctgaaaatt
    12121 gaggtggtgc gctataaccc ggaagtcgat accgcaccgc atagcgcatt ctatgaagtg
    12181 ccttatgacg caactacctc attactggat gcgctgggct acatcaaaga caacctggca
    12241 ccggacctga gctaccgctg gtcctgccgt atggcgattt gtggttcctg cggcatgatg
    12301 gttaacaacg tgccaaaact ggcatgtaaa accttcctgc gtgattacac cgacggtatg
    12361 aaggttgaag cgttagctaa cttcccgatt gaacgcgatc tggtggtcga tatgacccac
    12421 ttcatcgaaa gtctggaagc gatcaaaccg tacatcatcg gcaactcccg caccgcggat
    12481 cagggtacta acatccagac cccggcgcag atggccaagt atcaccagtt ctccggttgc
    12541 atcaactgtg gtctgtgcta cgccgcgtgc ccgcagtttg gcctgaaccc agagttcatc
    12601 ggtccggctg ccattacgct ggcgcatcgt tataacgaag atagccgcga ccacggtaag
    12661 aaggagcgta tggcgcagtt gaacagccag aacggcgtat ggagctgtac tttcgtgggc
    12721 tactgctccg aagtctgccc gaaacacgtc gatccggctg cggccattca gcagggcaaa
    12781 gtagaaagtt cgaaagactt tcttatcgcg accctgaaac cacgctaagg agtgcaacat
    12841 gacgactaaa cgtaaaccgt atgtacggcc aatgacgtcc acctggtgga aaaaattgcc
    12901 gttttatcgc ttctacatgc tgcgcgaagg cacggcggtt ccggctgtgt ggttcagcat
    12961 tgaactgatt ttcgggctgt ttgccctgaa aaatggcccg gaagcctggg cgggattcgt
    13021 cgacttttta caaaacccgg ttatcgtgat cattaacctg atcactctgg cggcagccct
    13081 gctgcacacc aaaacctggt ttgagctggc accaaaagcg gccaatatca ttgtaaaaga
    13141 cgaaaaaatg ggaccagagc caattatcaa aagcctctgg gcggtaactg tggttgccac
    13201 catcgtaatc ctgtttgttg ccctgtactg gtaaggagcc tgagatgatt aatccaaatc
    13261 caaagcgttc tgacgaaccg gtattctggg gcctcttcgg ggccggtggt atgtggagcg
    13321 caattattgc gccggtgatg atcctgctgg ttggtattct gctgccactg gggctgtttc
    13381 cgggtgacgc actgagctac gagcgcgttc tggcgttcgc gcagagcttc attggtcgcg
    13441 tattcctgtt cctgatgatc gttctgccgc tgtggtgtgg tttacaccgt atgcaccacg
    13501 cgatgcacga tctgaaaatc cacgtacctg cgggcaaatg ggttttctac ggtctggctg
    13561 ctatcctgac agttgtcacg ctgattggtg tcgttacaat ctaacgcatc gccaatgtaa
    13621 atccggcccg cctatggcgg gccgttttgt atggaaacca gaccctatgt tcaaaatgac
    13681 gctctgcgcc ttattaatta ccgcctcttg ctccacattt gctgcccctc aacaaatcaa
    13741 cgatattgtg catcgcacaa ttaccccgct tatagagcaa caaaagatcc ccggtatagc
    13801 ggtggcggta atttatcagg gtaaacctta ttactttacc tggggctatg cggacatcgc
    13861 caaaaagcag cccgtcacac agcaaacgtt gtttgagtta ggttcggtca gcaaaacatt
    13921 tacgggcgtg cttggtggcg acgctattgc tcgaggggaa atcaagttaa gcgatcccgc
    13981 gacaaaatac tggcctgaac ttaccgctaa acaatggaat gggatcacac tattacatct
    14041 cgcgacctac accgctggcg gcctgccatt gcaggtgccg gatgacgtta aatcctcaag
    14101 cgacttgctg cgcttctatc aaaactggca acctgcatgg gctccaggaa cacaacgtct
    14161 gtatgccaac gccagtattg ggttgttcgg tgcactggcg gtgaaaccat ccggtttgag
    14221 ctttgaacag gcgatgcaaa ctcgtgtttt ccagccactc aaactcactc atacgtggat
    14281 taatgtgccg tccgcagaag aaaagaatta cgcctgggga tatcgcgaag gtaaggcagt
    14341 gcatgtttcg ccaggggcgt tagatgctga ggcttatggt gtgaagtcga ccattgaaga
    14401 tatggcccgc tgggtgcaaa gcaatttaaa accccttgat atcactgaga aaacgcttca
    14461 acaagggata caactggcac aatctcgcta ctggcaaacc ggcgatatgt atcagggcct
    14521 gggctgggaa atgctggact ggccggtcaa tcctgacatc atcattaacg gcagtgacaa
    14581 taaaattgca ctggcagcac gccccgtaaa agcgattacg cccccaactc ctgcagtacg
    14641 cgcatcatgg gtacataaaa cgggggcgac cggcggattt ggtagctatg tcgcgtttat
    14701 tccagaaaaa gagctgggta tcgtgatgct ggctaacaaa aactatccca atccagcgag
    14761 agtcgccgcc gcctgtcaga ttctcaacgc tctacagtaa aatttcatcg ggtccgaatt
    14821 tttggacctt ttctccgctt ttccttgctg tcatctacac ttagaaaaaa accagtaagg
    14881 aaacatttat gcgcctgctc cctctcgttg ccgcagcgac agctgcattt ctggtcgttg
    14941 cctgcagttc tcctacgccg ccccgtggcg tgaccgtagt gaataatttc gatgctaaac
    15001 gctatctcgg tacctggtat gagattgccc gttttgatca ccgttttgaa cgtggactgg
    15061 aaaaagtcac cgcaacatac agcctgcgtg atgacggcgg cctgaatgtc attaataaag
    15121 gctataaccc tgacagagaa atgtggcagc agagtgaagg gaaagcgtac tttaccggcg
    15181 caccaactcg cgctgcgctg aaagtgtcat tctttggtcc tttctatggc ggttataacg
    15241 ttattgcact cgatcgggaa taccgccatg cgctggtttg cggtccggac cgcgactacc
    15301 tgtggatact ctcccgcacg ccaaccattt ctgacgaagt gaaacaggag atgctggcag
    15361 tcgcgacccg ggaagggttt gatgtcagta agtttatttg ggtacagcag cccggtagtt
    15421 agttagtgag tgctgagttt cagaccaata atccccaata cgattaacgc cagactcgcc
    15481 aggcgcatcg ggttagcgga ctcgccgagc agcacaatgc cggtgatggc cgcgccgact
    15541 gcgccaatac ccgtccacac ggcataagcc gtccctactg gtaacgattt catcgcccag
    15601 gcaagtagcg ccatactgac aatcatcgcc gtcacagtaa taacacttgg cgtcaaacga
    15661 ctaaagccgt gggtatattt caggccaacg gcccatacca cttccagcag gccagcaata
    15721 actaagataa tccaggacat atcaggctcc ggagaattgg ggccgtcccc ggtgaaaaga
    15781 ggcgtttgca ggtcgtcctg caaagctgat ggttgaacgg atattttgcc aggtggcagg
    15841 ggaatttcaa ctattacctt tgtgactatc gcaaaaaaac aaacggcacg acacagctgt
    15901 cgtaccgtta ttgcttactg ctgcgctttc gttgctgcgc cagaaatcgc gttaccgcca
    15961 tcagaaatgt cttcaccaac gccacgcgtg gtgttgcaag cagttaatac tgttgaaagc
    16021 accagaacag aaaagatcgc tgcaattgtc ttcttcacca taacgtcttc cttttaggta
    16081 atgttattta tgtttgccta tagcaaatta ataatagaca acatcgccaa aaatgatgga
    16141 aatctgatga tttttcggag gagaagaaaa attagctggc agcgcgggag atggagttgc
    16201 cgagatgttt aatgtcttca ccaaaaccgc gagcggtgtt acagcccgtg agcagcgcac
    16261 tggcaagcaa aacaagaacg ataaggcgtt tcatcatctc tgacctgaat aagtgatggt
    16321 gcagcctgca ggccgcacca caaccgcatt acttcacgcg agagacgtat tcaccagagc
    16381 gggtatccac tttgatgact tcgccgatct gtacgaacag cggaacttta accacagcgc
    16441 cagtagacag ggtagctggt ttgccgccag tacctgcggt atcacctttc aggcccggat
    16501 cggtatcaac gatttccagc tcaacgaagt tcggcggagt aacggagatc ggctgaccat
    16561 tccacagagt tacgatacac tctgcctgat ccagcaacca tttggcgttg tcgccaattg
    16621 cttttgcatc agcagacagc tgctcgaaag tttcgttgtt catgaagtgc cagaactcac
    16681 cgtcgttgta caggtaagtc aggttcatat cgacaacatc agcaccttca gcggaatcag
    16741 tagatttgaa ggttttttct acgcgagtac cggtcagcag acgacgcagt ttaacgcgag
    16801 caaatgcctg gcctttaccc ggttttacga attcactcgc ttcaaccgcg taaggttcgc
    16861 cgtctaacat gattttaaga ccagcacgaa aatcgttgct atagtacgtt gccataaggc
    16921 cctctgaaat ttgttaattg gtagctaagc cacaaaatgg cgcatattgt aaccctaaat
    16981 accccatcca gagaagattg gttaacgcaa cttgccgatg ttgtgaccga tcctgatgaa
    17041 cttctgcgtc ttttgaatat agacgcggac gaaaaactgt tggccgggcg cagcgccaaa
    17101 aagctgtttg ccctgcgtgt gccccgctca tttatcgatc gcatggagaa aggcaatccg
    17161 aacgatccgc ttttgcgtca ggtacttacc tcgcaagatg agtttgttgt cgcgcccgga
    17221 ttctccaccg acccgctgga agaacagcac agcgtagtgc caggtttgtt gcataaatac
    17281 cacaaccggg cgcttttgct ggtcaaaggc ggctgcgcgg taaattgccg ctattgcttc
    17341 cgtcgccact tcccctatgc cgaaaatcag ggcaacaagc gtaactggca gactgcactt
    17401 gagtatgttg ctgcgcatcc ggaactggac gagatgattt tctccggcgg cgatccgctg
    17461 atggcgaaag atcacgagct ggactggttg ctcacacaac tggaagccat cccgcatatc
    17521 aaacgtctgc ggattcacag ccgtctgccg attgtgatcc cggcacgcat cacagatgcg
    17581 ctggttgaac gcttttctca ttctacgctg caaatcttgc tggtgaatca cattaaccat
    17641 gccaatgagg tagatgaaac attccgtcag gcgatggcta agttgcgccg ggtaggcgtt
    17701 actttgctca accagagcgt tctgttacgt ggtgtgaacg ataacgcgca aacgctggca
    17761 aacctgagta atgcgttgtt cgatgcgggc gtgatgccgt attacctgca tgtgctcgat
    17821 aaagtacagg gcgcggcgca ttttatggtg agtgatgacg aagcacggca gattatgcgt
    17881 gagttgctga cactggtgtc gggatatctg gtgcctaaac tggcgcgaga aattggcggc
    17941 gaacccagca aaacgccgct ggatctccag ctacgccagc agtaactgat taaacaggga
    18001 agtttaatct ttgcacgttg cataaccttt aattattcat atacattgct gttattactt
    18061 tttatataac caacaccaat aattaacctt tcttttctgt ttatatagag tgagcacggc
    18121 tatttatgtt attttaacaa ctattactac caagcccatt tatctggctt ataaattaaa
    18181 attaattatt taattgagaa atggttaggg agaacctaca tggccataag catcaagggt
    18241 gttaataccg gtgttattcg caagagcaat aatttcattg cactggcgct gaaaattaaa
    18301 gagcctcgca ataaagaatc gttgttcttt ctgtctgtca tggaactgcg cgaccttctg
    18361 attgcgctgg aaagccgtct gcatcaaaag cataaactgg atgctgctgc acgccttcag
    18421 tatgagcaag ctcgcgataa agtcattaag aaaatggtag aaaatatccc agaaattctg
    18481 gttgatgagc ttaaaaatgc cgatattaat cgtcgggtta acacgctgga acttaccgat
    18541 aatcagggcg aaaatcttac cttcgtatta acactacatg acggtagcaa atgtgagtta
    18601 gtcatcaatg aattacagat tgaaatgctg gcgcgagcaa tcattcatgc aattaataat
    18661 gcggaaatgc gtgaactggc attacgtatt acttcactgt tagatttcct gccgttatac
    18721 gacgttgact gccaggacaa tggcaatctg gaatatgaca cctattccca accggaatgg
    18781 aaacataact tgttcaacca ttatctggcg gtgctttacc gctttaaaga tgaaagtggc
    18841 aaagaacaat ttagcggcgc agtggtgaaa acgcgtgaag caacgccggg taaagaagtg
    18901 gaggcgatca ctcgccgaat gcttgatttc agcccccgac tgaaaaaact cgcgggtgtg
    18961 ccatgtcagg tctatgttcg caccgtagcg gcaaataacg cccaaccgct aacccaggat
    19021 caatgcttac gcgcgctgca tcacttacgt gttcaatcca ccagcaaaac tgcaccacag
    19081 gcgaaataat ctcatctgtt ttactacgag gctggaagta tccagcctct ggatcacatc
    19141 agttcgggca cttataaacc tgaccgataa tctggctatc cgtcgggaca aaactggaca
    19201 acattccctg cgacgggctg ctgatgccat aaatcacgtt accgcccatt gcagccgcct
    19261 ggttgcgcag atcgtttgct gcgccgcgca tagaaccgcc ctcttctccg tgttgcccgg
    19321 aaagccagtt gctttgctta cctgtcgcag taccaatcag ctggcactct gcgcctggct
    19381 gctcgtccac aatgcgtaca ctctgaccgg cagcactcaa ttcgttgctg gagctacaac
    19441 ccgccatcag tagcgcggca ccgacaatcc ctgctaagta ttttacgtgc atgttattcc
    19501 ccataatcaa tgagctggac gccactcgcg tcgtccgtgt ctgaatctta tactagaaag
    19561 atgaccaaaa gaaaaacccc cagacatttc tgcccggggg tttgtttatt tctgcgaggt
    19621 gcagggcaat tacatcatgc cgcccatgcc acccatgccg cccataccgc cagcagcgcc
    19681 taagtcagct gcatcgtttt tcggcaggtc ggtaaccatg cattcggtgg tgatcatcag
    19741 gccagccaca gaagctgcgt actgcagagc agaacgagtt actttggttg gatccaggat
    19801 acccatgtcg atcatgttgc cgtattcttc ggttgctgcg ttgtaaccgt agttgccgtc
    19861 gccgccttta acggtgttag caacaacaga cggttcttcg ccgcagttca atacgatctg
    19921 acgcagcgga gcttccattg cacgcagtgc aactttgata cccacgttct ggtcttcgtt
    19981 ctgaccacgc aggtcagcca gtttagacgc tacgcggatc agcgcaacac caccaccagc
    20041 aaccacgcct tcttctaccg cagcacgggt cgcgtgcagg gcatcttcaa cgcgtgcttt
    20101 tttctctttc atttcaactt cggtagcagc acccactttg ataactgcaa cgccgcctgc
    20161 cagtttcgct acgcgctcct gcagtttttc acggtcgtag tcagaagttg cttcttcaat
    20221 ctgctgacgg atctgagcaa cacggccctg gattgcagct tcttcaccca cgccatcaat
    20281 gatggtggtg gtgtctttgt tgatcacaac acgtttagcc tgacccaggt cttccagggt
    20341 tgctttttcc agctccatac cgatctcttc agagattacg gtaccgccag tcagggttgc
    20401 gatatcctgc agcatagctt tacgacgatc gccgaagccc ggagctttaa ccgcagcaac
    20461 tttcacgatg ccacgcatgg tgttaacaac cagagttgcc agcgcttcgc cttctacatc
    20521 ttcagcgatg atcagcagcg gtttgcctgc tttggcaaca gcttccagaa ccggcagcat
    20581 ttcgcggatg ttggagattt tcttgtcagc cagcaggatg aacgggcttt ccagttctac
    20641 tgcgccagtt tccggcttgt tgataaagta aggagacagg tagccacggt cgaactgcat
    20701 accttcaacc acgtccagtt cgtcctgcag accggtaccg tcttcaacgg tgataacgcc
    20761 ttctttaccg actttgtcca tcgcttcagc gatcagttta cctacggttt cgtcggagtt
    20821 agcggagatg gtaccaacct gagcaatcgc tttagagtca gagcatggta cggacagcgc
    20881 tttcagttct tcaactgcag cggtaaccgc tttgtcgata ccacgtttca ggtccatcgg
    20941 gttcatgccc gcagcaacag ctttcagacc ttcagtgatg atagcctgag ccagtacggt
    21001 tgcagtggtg gtaccgtcgc ctgcagcgtc gttcgctttg gaggcaactt ctttcaccat
    21061 ctgcgcaccc atgttttcga acttgtcttc cagttcgatt tcacgagcaa cggaaacacc
    21121 atctttggtg atggtcggtg caccgaaaga tttatccaga actacgttac ggcctttcgg
    21181 accgagggta actttcactg catctgccag tacgtttacg ccgcgcagca ttttcacacg
    21241 agcgtcgtta ccgaatttta cgtctttagc tgccattatc tttattcctt aaattcgtat
    21301 gttcagtgtc gtgcgcggat tacgcttcaa caattgccag aatgtcgctt tcggacatga
    21361 tcaacacttc ttcattgtcg atcttctcgg atttcacacc gtagccatcg ttgaaaataa
    21421 cgatgtcgcc aactttcacg tccagcggct tcacttcgcc attttcaagg atacggccat
    21481 tgccgacagc cagcacttcg ccgcgggtgg atttagccgc tgcagagccg gtcagaacga
    21541 tgccgccagc agatttagtt tcaacttctt tacgcttgac gatcacgcga tcatgcaatg
    21601 gacgaatatt cattgataac tctcctttga gaaagtccgt atctgttatg ggtgacgccg
    21661 gagcttacgt ggtttcccgg ctggtgacca gagaaatggg gatgaggctt cgccccttca
    21721 agggggaaaa agaaaaaaaa ttctgatcag cacaaaatcg ggtgaaaacc ctgattcacc
    21781 tcacatttca tcgcaatttc ttcatcgccg tcataagcga atctgattgt gctaccatcg
    21841 aaaatctacg cagttgccca aaatttgggc gcaatcggac atcaacccat gagtggactc
    21901 aaacaagaac tggggctggc ccagggcatt ggcctgctat cgacgtcatt attaggcact
    21961 ggcgtgtttg ccgttcctgc gttagctgca ctggtagcgg gcaataacag cctgtgggcg
    22021 tggcccgttt tgattatctt agtgttcccg attgcgattg tgtttgcgat tctgggtcgc
    22081 cactatccca gcgcaggcgg cgtcgcgcac ttcgtcggta tggcgtttgg ttcgcggctt
    22141 gagcgagtca ccggctggct gtttttatcg gtcattcccg tgggtttgcc tgccgcacta
    22201 caaattgccg ccgggttcgg ccaggcaatg tttggctggc atagctggca actgttgttg
    22261 gcagaactcg gtacgctggc gttggtgtgg tatatcggta ctcgcggtgc cagttccagt
    22321 gcaaatctac aaaccgttat tgccggactt atcgtcgcgc tgattgtcgc tatctggtgg
    22381 gcgggcgata tcaaacctgc gaatatcccc ttcccggcac ctggtaatat cgaacttacc
    22441 gggttatttg ctgcgttatc agtgatgttc tggtgttttg tcggtctgga ggcatttgcc
    22501 catctcgcct cggaatttaa aaatccagag cgtgattttc ctcgtgcttt gatgattggt
    22561 ctgctgctgg caggattagt ctactgggga tgtacggtag tcgtcttaca cttcgacgcc
    22621 tatggtgaaa aaatggcggc ggcagcatcg cttccaaaaa ttgtagtgca gttgttcggt
    22681 gtaggagcgt tatggattgc ctgcgtgatt ggctatctgg cctgctttgc cagtctcaac
    22741 atttatatac agagcttcgc ccgcctggtc tggtcgcagg cgcaacataa tcctgaccac
    22801 tacctgtcac gcctctcttc tcgccatatc ccgaataatg ccctcaatgc ggtgctcggc
    22861 tgctgcgtgg tgagcacttt ggtgattcat gctttagaga tcaatctgga cgctcttatt
    22921 atttatgcca atggcatctt tattatgatt tatctgttat gcatgctggc aggctgtaaa
    22981 ttattgcaag gacgttatcg actactggcg gtggttggcg ggctgttatg cgttctgtta
    23041 ctgacaatgg tcggctggaa aagtctctat gcgctgatca tgctggcggg gttatggctg
    23101 tttctgccaa aacgaaaaac gccggaaaat ggcataacca cataatccgg cgtttcgaca
    23161 ttaatcctgg cgatcgtctt tatgatcaag gcggtcgcgg tcatcatcct ttcgctggta
    23221 ctcaccatca aaagtattac cgccaccggt cccggcgcta aaaccgccgc ctggcatgcg
    23281 agaaaagcgc aaatgcggca tcaacttcac tgtcagatgc ttttgcaccg gcggcaacaa
    23341 aagcagaaga ccgaggaagt cggtgaaaaa gcccggtaat aaaagcagca aaccggcaat
    23401 gatcagcgaa acacttttaa tcatctccgc cgctgggttt tcacccgccg ccattttttg
    23461 ctgcatcagc acgaaattct taaagccctg gttacgtacc agtgacatac cgataactga
    23521 cgtgaatata accagcacga gagtcagcaa tacccccaat acatgggcaa cctgaataaa
    23581 gattgaaatc tcaatataga cataaaggaa aatggcaata aaaggtaacc agcgcaaagg
    23641 tttctcctgt aatagcagcc ggttaaaccc ggctacctga atgggttgcg aatcgcgttc
    23701 agcttatatt gtggtcatta gcaaaatttc aagatgtttg cgcaactatt tttggtagta
    23761 atcccaaagc ggtgatctat ttcacaaatt aataattaag gggtaaaaac cgacacttaa
    23821 agtgatccag attacggtag aaatcctcaa gcagcatatg atctcgggta ttcggtcgat
    23881 gcaggggata atcgtcggtc gaaaaacatt cgaaaccaca tatattctgt gtgtttaaag
    23941 caaatcattg gcagcttgaa aaagaaggtt cacatgtcaa acaacattcg tatcgaagaa
    24001 gatctgttgg gtaccaggga agttccagct gatgcctact atggtgttca cactctgaga
    24061 gcgattgaaa acttctatat cagcaacaac aaaatcagtg atattcctga atttgttcgc
    24121 ggtatggtaa tggttaaaaa agccgcagct atggcaaaca aagagctgca aaccattcct
    24181 aaaagtgtag cgaatgccat cattgccgca tgtgatgaag tcctgaacaa cggaaaatgc
    24241 atggatcagt tcccggtaga cgtctaccag ggcggcgcag gtacttccgt aaacatgaac
    24301 accaacgaag tgctggccaa tatcggtctg gaactgatgg gtcaccagaa aggtgaatat
    24361 cagtacctga acccgaacga ccatgttaac aaatgtcagt ccactaacga cgcctacccg
    24421 accggtttcc gtatcgcagt ttactcttcc ctgattaagc tggtagatgc gattaaccaa
    24481 ctgcgtgaag gctttgaacg taaagctgtc gaattccagg acatcctgaa aatgggtcgt
    24541 acccagctgc aggacgcagt accgatgacc ctcggtcagg aattccgcgc tttcagcatc
    24601 ctgctgaaag aagaagtgaa aaacatccaa cgtaccgctg aactgctgct ggaagttaac
    24661 cttggcgcaa cagcaatcgg tactggtctg aacacgccga aagagtactc tccgctggca
    24721 gtgaaaaaac tggctgaagt cactggcttc ccatgcgtac cggctgaaga cctgatcgaa
    24781 gcgacctctg actgcggcgc ttatgttatg gttcacggcg cgctgaaacg cctggctgtg
    24841 aagatgtcca aaatctgtaa cgacctgcgc ttgctctctt caggcccacg tgccggcctg
    24901 aacgagatca acctgccgga actgcaggcg ggctcttcca tcatgccagc taaagtaaac
    24961 ccggttgttc cggaagtggt taaccaggta tgcttcaaag tcatcggtaa cgacaccact
    25021 gttaccatgg cagcagaagc aggtcagctg cagttgaacg ttatggagcc ggtcattggc
    25081 caggccatgt tcgaatccgt tcacattctg accaacgctt gctacaacct gctggaaaaa
    25141 tgcattaacg gcatcactgc taacaaagaa gtgtgcgaag gttacgttta caactctatc
    25201 ggtatcgtta cttacctgaa cccgttcatc ggtcaccaca acggtgacat cgtgggtaaa
    25261 atctgtgccg aaaccggtaa gagtgtacgt gaagtcgttc tggaacgcgg tctgttgact
    25321 gaagcggaac ttgacgatat tttctccgta cagaatctga tgcacccggc ttacaaagca
    25381 aaacgctata ctgatgaaag cgaacagtaa tcgtacaggg tagtacaaat aaaaaaggca
    25441 cgtcagatga cgtgcctttt ttcttgtgag cagtaactta aaaataacaa tctaatatca
    25501 acttgttaaa aaaacaagga aggctaatat gctagttgta gaactcatca tagttttgct
    25561 ggcgatcttc ttgggcgcca gattgggggg aataggtatt ggttttgcag gcggattggg
    25621 ggtgctggtt cttgccgcta ttggcgttaa acccggtaac atcccgttcg atgtcatttc
    25681 cattatcatg gcggttatcg ccgctatttc tgccatgcag gttgctggcg gtctggacta
    25741 tctggttcat cagacagaaa agctgctgcg ccgtaacccg aaatacatca cgatcctcgc
    25801 accgatcgtg acctatttcc tgacaatctt tgctggtact ggcaacatct ctctggcgac
    25861 actgccagtt atcgctgaag ttgcgaagga acaaggcgtt aaaccttgcc gtccgctgtc
    25921 tactgcagtg gtatccgcgc agattgcgat caccgcatcg ccaatctcag cggcagtggt
    25981 ttacatgtct tccgtaatgg aaggtcatgg catcagctac ctccatctgc tctccgtggt
    26041 catcccgtcc accctgctgg cggttctggt gatgtccttc ctggtcacta tgctgttcaa
    26101 ctccaaactc tctgacgatc cgatttatcg caagcgtctg gaagaaggcc tggttgaact
    26161 gcgcggtgaa aagcagattg aaatcaaatc cggtgcaaaa acgtccgtct ggctgttcct
    26221 gctgggcgta gttggcgttg ttatctatgc aatcatcaac agcccaagca tgggtctggt
    26281 tgaaaaaccg ctgatgaaca ccaccaacgc aatcctgatc atcatgctca gcgttgcaac
    26341 tctgaccacc gttatctgta aagtcgatac cgacaacatc ctcaactcca gcaccttcaa
    26401 agcaggtatg agcgcctgta tttgtatcct gggtgttgcg tggctgggcg atactttcgt
    26461 ttccaacaac atcgactgga tcaaagatac cgctggtgaa gtgattcagg gtcatccgtg
    26521 gctgctggcc gtcatcttct tctttgcttc tgctctgctg tactctcagg ctgcaaccgc
    26581 aaaagcactg atgccgatgg ctctggcact gaacgtttct ccgctgaccg ctgttgcttc
    26641 tttcgctgcg gtgtctggtc tgttcattct gccgacctac ccgacgctgg ttgctgcggt
    26701 acagatggat gacacgggta ctacccgtat cggtaaattc gtcttcaacc atccgttctt
    26761 catcccgggt actctgggtg ttgccctggc cgtttgcttc ggcttcgtgc tgggtagctt
    26821 catgctgtaa tgatcaatcg cggggcgttc acgccccgct ttctttcccg ccgactaaca
    26881 tccttccccc gtccgttgta tagtgacctc tctcttgcgg ttccatctgt tcttgcgagg
    26941 tgtttatgct tgatgaaaaa agttcgaata ccgcgtctgt cgtggtgcta tgtaccgcac
    27001 cggatgaagc gacagcccag gatttagccg ccaaagtgct ggcggaaaaa ctggcggcct
    27061 gcgcgacctt gatccccggc gctacttctc tctattactg ggaaggtaag ctggagcaag
    27121 aatacgaagt gcagatgatt ttaaaaacta ccgtatctca ccagcaggca ctgctggaat
    27181 gcctgaagtc tcatcatcca tatcaaaccc cggaacttct ggttttacct gttacacacg
    27241 gagacacaga ttacctctca tggctcaacg catctttacg ctgatcctgc tactttgcag
    27301 cacttccgtt tttgccggat tattcgacgc gccgggacgt tcacaatttg tccccgcgga
    27361 tcaagccttt gcttttgatt ttcagcaaaa ccaacatgac ctgaatctga cctggcagat
    27421 caaagacggt tattacctct accgtaaaca gatccgcatt acgccggaac acgcgaaaat
    27481 tgccaacgtg cagctgccac aaggcgtctg gcatgaagat gagttttacg gcaaaagcga
    27541 gatttaccgc gatcggctga cgcttcccgt aaccatcaac caggcgagtg cgggagcgac
    27601 gttaactgtc acctaccagg gctgtgctga tgccggtttc tgttatccgc cagaaaccaa
    27661 aaccgttccg ttaagcgaag tggtcgccaa caacgcagcg tcacagcctg tgtctgttcc
    27721 gcagcaagag cagcccaccg cgcaattgcc cttttccgcg ctctgggcgt tgttgatcgg
    27781 tattggtatc gcctttacgc catgcgtgct gccaatgtac ccactgattt ctggtatcgt
    27841 gctgggtggt aaacagcggc tctccactgc cagagcattg ttgctgacct ttatttatgt
    27901 gcaggggatg gcactgactt acacggcgct gggtctggta gttgccgccg cagggttaca
    27961 gttccaggcg gcgctacagc acccatacgt gctcattggc ctcgccatcg tctttacctt
    28021 gctggcgatg tcaatgtttg gtttgtttac cctgcaactc ccctcttcgc tgcaaacgcg
    28081 cctcacgctg atgagcaatc gccaacaggg cggctcacct ggcggtgtgt ttgttatggg
    28141 agcgattgcc ggactgatct gttcaccatg caccaccgca ccgcttagcg cgattctgtt
    28201 gtatatcgcc caaagcggga acatgtggct ggggggcggc acgctttatc tctatgcgtt
    28261 gggcatgggc ctgccgctga tgctaataac cgtctttggt aaccgcctgc tgccgaaaag
    28321 cggcccgtgg atggaacaag tcaaaaccgc gtttggtttt gtgatcctcg cactgccggt
    28381 cttcctgctg gagcgagtga ttggcgatat atggggatta cgcttgtggt cggcgcttgg
    28441 tgtcgcattc tttggctggg cctttatcac cagcctacag gccaaacgcg gctggatgcg
    28501 cgtggtgcaa ataatcctgc tggcagcggc attggttagc gtgcgcccac ttcaggattg
    28561 ggcatttggt gcaacacata ccgcgcaaac tcagacgcat ctcaacttta cacaaatcaa
    28621 aacggtagat gagttaaatc aggcgctcgt tgaagccaaa ggcaaaccag tgatgttaga
    28681 tctctatgcc gactggtgcg tcgcctgtaa agagtttgag aaatacacct tcagcgaccc
    28741 gcaggtgcaa aaagcgttag cagacacggt cttacttcag gctaacgtaa ctgccaacga
    28801 cgcacaagat gtggcgctgt taaagcatct taatgtcctt ggcctaccga caattctctt
    28861 ttttgacgga caaggccagg agcatccaca agcacgcgtc acgggcttta tggatgctga
    28921 aaccttcagc gcacatttgc gcgatcgcca accgtgaacg acacttgcag tgggaaagac
    28981 ggaggaaaat agcgtgcaac gtgaagatgt actgggagaa gccctgaaat tattagaatt
    29041 acaagggatt gccaacacca cgcttgagat ggttgctgaa cgtgtggatt acccactgga
    29101 cgagctacgc cgcttctggc cagacaaaga ggcaatcctc tacgatgcgc tgcgttatct
    29161 tagccaacag atagatgcct ggcgtcgtca gctgatgctg gacgaaacgc aaaccgccga
    29221 acaaaagctg ctggcacgtt atcaggcgtt atcggagtgc gttaaaaaca accgctatcc
    29281 gggctgtcta tttatcgctg cctgtacgtt ttatcccgat cctggccacc ctattcatca
    29341 actggccgat cagcaaaaaa gcgcggccta cgatttcacc cacgaactgt taaccacact
    29401 ggaagttgac gatccggcga tggtagcaaa gcagatggaa ctggtgctgg aaggctgttt
    29461 aagccgaatg ctggtgaatc gtagccaggc ggatgtcgac accgcacatc ggctggcgga
    29521 agatatcctg cgcttcgccc gctgccgcca gggtggtgct ttgacctgac agaaacacag
    29581 aaaagaagcg atttgccgca atcttaagca gttgaatcgc ttttactgaa attaggttga
    29641 cgagatgtgc agattacggt ttaatgcgcc ccgttgcccg gatagctcag tcggtagagc
    29701 aggggattga aaatccccgt gtcggtggtt cgattccgcc tccgggcacc atataccaga
    29761 acacgctggt taagggatga gatccttgac gggcggttaa tacaggatgc ggcatcaaag
    29821 ggtctccgtt tgtgtaagtt gtggttacac tgattgagtt cggtatcgta tcctcaaaca
    29881 gggtgtcttt tgcggagacg ccctgttctc gttaacactg tatgcttaca tcgctgattc
    29941 agcctgtcaa gatttattcc cccctttgca aatcttgctc actgtgcgct catctgtata
    30001 caaactcagg gcatcttcca gtgtctg
//
LOCUS       DAZGQL010000067        29685 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.067, whole genome shotgun sequence.
ACCESSION   DAZGQL010000067 DAZGQL010000000
VERSION     DAZGQL010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29685
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.067"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            151..843
                     /gene="yieP"
                     /locus_tag="ACHKIR_003232"
     CDS             151..843
                     /gene="yieP"
                     /locus_tag="ACHKIR_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFO9990919.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAAMVGTAEQKAHLNTLMTEMVALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
     gene            866..2293
                     /gene="mdtD"
                     /locus_tag="ACHKIR_003233"
     CDS             866..2293
                     /gene="mdtD"
                     /locus_tag="ACHKIR_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HFO9990920.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(2259..3251)
                     /gene="rbsR"
                     /locus_tag="ACHKIR_003234"
     CDS             complement(2259..3251)
                     /gene="rbsR"
                     /locus_tag="ACHKIR_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HFO9990921.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLLHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(3255..4184)
                     /gene="rbsK"
                     /locus_tag="ACHKIR_003235"
     CDS             complement(3255..4184)
                     /gene="rbsK"
                     /locus_tag="ACHKIR_003235"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFO9990922.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDISPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            complement(4310..5200)
                     /gene="rbsB"
                     /locus_tag="ACHKIR_003236"
     CDS             complement(4310..5200)
                     /gene="rbsB"
                     /locus_tag="ACHKIR_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HFO9990923.1"
                     /translation="MNMKKLATLVSAVALSATVSANAMAKDTIALVVSTLNNPFFVSL
                     KDGAQKEADKLGYNLVVLDSQNNPAKELANVQDLTVRGTKILLINPTDSDAVGNAVKM
                     ANQANIPVITLDRQATKGEVVSHIASDNVLGGKIAGDYIAKKAGEGAKVIELQGIAGT
                     SAARERGEGFQQAVAAHKFNVLASQPADFDRTKGLNVMQNLLTAHPDVQAVFAQNDEM
                     ALGALRALQTAGKSDVMVVGFDGTPDGEKAVNDGKLAATIAQLPDQIGAKGVETADKV
                     LKGEKVQAKYPVDLKLVVKQ"
     gene            complement(5225..6190)
                     /gene="rbsC"
                     /locus_tag="ACHKIR_003237"
     CDS             complement(5225..6190)
                     /gene="rbsC"
                     /locus_tag="ACHKIR_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HFO9990924.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            complement(6195..7700)
                     /gene="rbsA"
                     /locus_tag="ACHKIR_003238"
     CDS             complement(6195..7700)
                     /gene="rbsA"
                     /locus_tag="ACHKIR_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HFO9990925.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            complement(7708..8127)
                     /gene="rbsD"
                     /locus_tag="ACHKIR_003239"
     CDS             complement(7708..8127)
                     /gene="rbsD"
                     /locus_tag="ACHKIR_003239"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFO9990926.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPHLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            complement(8294..10162)
                     /gene="kup"
                     /locus_tag="ACHKIR_003240"
     CDS             complement(8294..10162)
                     /gene="kup"
                     /locus_tag="ACHKIR_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HFO9990927.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            10385..11881
                     /gene="ravA"
                     /locus_tag="ACHKIR_003241"
     CDS             10385..11881
                     /gene="ravA"
                     /locus_tag="ACHKIR_003241"
                     /EC_number="3.6.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HFO9990928.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPASLQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERNALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
     gene            11875..13326
                     /gene="viaA"
                     /locus_tag="ACHKIR_003242"
     CDS             11875..13326
                     /gene="viaA"
                     /locus_tag="ACHKIR_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HFO9990929.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCMALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(13331..14323)
                     /gene="asnA"
                     /locus_tag="ACHKIR_003243"
     CDS             complement(13331..14323)
                     /gene="asnA"
                     /locus_tag="ACHKIR_003243"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HFO9990930.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLEL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            14475..14933
                     /gene="asnC"
                     /locus_tag="ACHKIR_003244"
     CDS             14475..14933
                     /gene="asnC"
                     /locus_tag="ACHKIR_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HFO9990931.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            15023..15466
                     /gene="mioC"
                     /locus_tag="ACHKIR_003245"
     CDS             15023..15466
                     /gene="mioC"
                     /locus_tag="ACHKIR_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HFO9990932.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            15845..17734
                     /gene="mnmG"
                     /locus_tag="ACHKIR_003246"
     CDS             15845..17734
                     /gene="mnmG"
                     /locus_tag="ACHKIR_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFO9990933.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            17798..18421
                     /gene="rsmG"
                     /locus_tag="ACHKIR_003247"
     CDS             17798..18421
                     /gene="rsmG"
                     /locus_tag="ACHKIR_003247"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFO9990934.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLHVPALDGERHLVVIKANKI"
     gene            19038..19418
                     /gene="atpI"
                     /locus_tag="ACHKIR_003248"
     CDS             19038..19418
                     /gene="atpI"
                     /locus_tag="ACHKIR_003248"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HFO9990935.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            19427..20242
                     /gene="atpB"
                     /locus_tag="ACHKIR_003249"
     CDS             19427..20242
                     /gene="atpB"
                     /locus_tag="ACHKIR_003249"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFO9990936.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPHNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            20289..20528
                     /gene="atpE"
                     /locus_tag="ACHKIR_003250"
     CDS             20289..20528
                     /gene="atpE"
                     /locus_tag="ACHKIR_003250"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFO9990937.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            20590..21060
                     /gene="atpF"
                     /locus_tag="ACHKIR_003251"
     CDS             20590..21060
                     /gene="atpF"
                     /locus_tag="ACHKIR_003251"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFO9990938.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            21075..21608
                     /gene="atpH"
                     /locus_tag="ACHKIR_003252"
     CDS             21075..21608
                     /gene="atpH"
                     /locus_tag="ACHKIR_003252"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFO9990939.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            21621..23162
                     /gene="atpA"
                     /locus_tag="ACHKIR_003253"
     CDS             21621..23162
                     /gene="atpA"
                     /locus_tag="ACHKIR_003253"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFO9990940.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            23213..24076
                     /gene="atpG"
                     /locus_tag="ACHKIR_003254"
     CDS             23213..24076
                     /gene="atpG"
                     /locus_tag="ACHKIR_003254"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFO9990941.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            24103..25485
                     /gene="atpD"
                     /locus_tag="ACHKIR_003255"
     CDS             24103..25485
                     /gene="atpD"
                     /locus_tag="ACHKIR_003255"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFO9990942.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            25506..25925
                     /gene="atpC"
                     /locus_tag="ACHKIR_003256"
     CDS             25506..25925
                     /gene="atpC"
                     /locus_tag="ACHKIR_003256"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFO9990943.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            26274..27644
                     /gene="glmU"
                     /locus_tag="ACHKIR_003257"
     CDS             26274..27644
                     /gene="glmU"
                     /locus_tag="ACHKIR_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFO9990944.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQSQKEGWR
                     RPVKKK"
     gene            27806..29635
                     /gene="glmS"
                     /locus_tag="ACHKIR_003258"
     CDS             27806..29635
                     /gene="glmS"
                     /locus_tag="ACHKIR_003258"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFO9990945.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
BASE COUNT         7337 a         7576 c         7624 g         7148 t
ORIGIN      
        1 ggaaatttaa aataattttc tgaccgcgca ttttttcagc aaaagccccg ttaatgtgcc
       61 ataaaacaag caatttgccg tttttgcaac cgtaatcaca cttcctggtg gcatactaag
      121 gaggaataaa aagaaaaagg aagattagta atgcctttaa gcgcacaaca gttagctgca
      181 cagaaaaacc tttcgtatgt tctggctgag aagctggcgc aacgcatctt aaaaggtgaa
      241 tatgaacccg gcaccatttt gcctggtgag attgagctgg gcgagcaatt tggagtgagt
      301 cgtacagcgg tacgcgaagc ggtcaaaacg ttaacggcaa aagggatggt tttaccgcga
      361 ccgcgaattg gtacccgggt catgccacaa tcaaactgga attttcttga tcaggaattg
      421 cttacctggt ggatgacaga agagaacttt catcaggtca tcgatcactt tctggttatg
      481 cgtatctgtc tggagccgca agcttgccta ctggcagcaa tggttggcac cgcggaacag
      541 aaggcgcatc tcaatacgtt aatgaccgaa atggtggcat taaaagagaa ctttcgccgt
      601 gaacgctgga ttgaggtcga tatggcctgg catgagcata tctatgaaat gagcgccaat
      661 ccgtttttga cctcattcgc ctcgctcttc cattcggttt atcacactta cttcacgtca
      721 attaccagcg acacagtgat aaagctggat ctgcaccagg cgattgttga cgcgattatc
      781 caaagcgatg gcgacgcggc atttaaagct tgtcaggcgc tgctacgctc acctgataag
      841 tgacaccccg ataacaggac attgaatgag cgataaaaag aagcgcagta tggcgggttt
      901 gccgtggatc gcggcgatgg ccttctttat gcaggcactt gatgccacta ttctgaatac
      961 cgccttaccc gcaatcgctc atagccttaa tcgttctcct ctcgcgatgc aatcagccat
     1021 catcagttat acgctgacgg tggcgatgct tattccggta agcggatggc tagccgatcg
     1081 cttcggtacg cgtcgcatat ttactcttgc cgtgagtctg ttcacgttgg gttctctggc
     1141 ctgcgcactt tctaactcgc taccacagct ggttgtcttc cgggttattc aggggatagg
     1201 cggcgcaatg atgatgcctg ttgctcggct ggccttactg cgcgcttatc ctcgtaatga
     1261 gcttcttcct gtattgaatt ttgtcgccat gccgggtctg gtggggccaa ttttaggccc
     1321 tgttcttggc ggcgtgctgg tcacctgggc aacctggcac tggatatttt taatcaatat
     1381 ccccataggt attgcgggcc ttctttacgc gcgcaaacat atgcccaatt tcaccaccgc
     1441 acgacgcaga ttcgatatca ctggcttttt gctgtttggc cttagccttg ttctcttctc
     1501 aagcggaata gagctattcg gggaaaagat tgtcgccagt tggattgcct tgacggtaat
     1561 tgtcaccagc atcgggttac tgcttctcta tattctccat gcacgacgca cgccaaaccc
     1621 attaatttca ttagatttat ttaaaacccg cactttctcg atcggtatcg taggcaatat
     1681 tgcaacccgt ctggggaccg gttgtgtacc gttccttatg ccattgatgt tacaggtagg
     1741 atttggttat caggcgttta ttgccggctg tatgatggca ccgacagcgt taggttccat
     1801 tattgcaaaa tcgatggtta cccaagtctt acgtcgtctg ggctatcgcc atacattagt
     1861 ggggatcacg gtgattattg ggctaatgat cgctcagttc tctttgcaat caccggcaat
     1921 ggcgatatgg atgctgatct tgccgttgtt tatattaggg atggctatgt cgacgcaatt
     1981 taccgcgatg aataccatca cacttgccga tctgaccgat gacaacgcca gcagcggtaa
     2041 cagtgttctg gcggtcacgc agcaactgtc tatcagttta ggcgttgctg taagtgcggc
     2101 cgtccttcgc gtttatgaag ggatggaagg cacaacgact gtcgaacaat tccactatac
     2161 gtttatcacg atgggcatta ttactgttgc ttcagcagca atgttcatgc ttctgaaaac
     2221 aaccgatggt aataatttga tcaaaagaca gcgtaaatct aagccgaacc gcgttccatc
     2281 agaatcggag taagttgtaa tcgttgttgc tgaagggtcg gttgggttat ccgatggatg
     2341 agtacatcaa tcgccagctc ccctagttca tctttcggtt ggtggatagt ggttaatggt
     2401 ggcgtcataa agcttgccag ttcgatatcg tcatagccaa tcaccgcaat atcctgcgga
     2461 acttgtaact ctgcctgata cagcgcctgg taaacgccaa cagccatagc gtcatttccg
     2521 gtaaagacgg cctgaggacg cagcggatgt aataacagtt ggcgcatagc gtcaaacccg
     2581 ccgttaaatt caaaatcacc agtgacttca tagccatcag gaatgttgag acccgcacgt
     2641 ttcatcgccg cccgataacc ttccaaccgc aggcgtgccg gagttttatc cagcgggccg
     2701 gtaatacagg cgatacgggt atgaccttta tcgatcagat attgcgttgc taagtctccg
     2761 cccagcaacg agttatcctg aataagatcg ctgtcgccat cgaacggagc ccagtccatc
     2821 atcacagtag gcactgtcgg ataacgttgc atgatttcac gcgaaggttg atgcgtttcg
     2881 gtgcacagta acagcaagcc atcaacgcgt ttttgcatca gcgtttccag attgcgattc
     2941 atccgctgtt catcgccttc ggtattgcaa aggacaagac tataaccgcg ttcgaagcaa
     3001 ctgcgttcaa cgccacgcac cagttcagaa tagaaaggat tggtactggc agtgatcaac
     3061 atgccaatgg tatgtgtttg attgagtttg aggctacgcg ccagggctga tggcgcgtaa
     3121 ttgagttctt taatcgccgc ttcaactttg gcggtaatcg cttcactgac gaagcgatct
     3181 ttattgataa cgtgagaaac tgttgaggta gaaacgcccg ccaggcgggc aacatctttc
     3241 attgtagcca agcgtcacct ctgcctgtct aaaaatgcgt cgatctcttc acgccacggt
     3301 acggaaggtt gtgcgccttt acgtgttacg gcaatcgcag cggcagcatg agcaaaccga
     3361 atcgcctctg gcaatggttt ttcttccagc aacgccgtga ttaacgcgcc gttaaaggta
     3421 tctccggcag caatggtatc gacagcctgc acccggaatc caggaacgcg ctgaccttca
     3481 ccattcacgc tagcccatac accacgactt cctaaagtaa tcagtacagt acggatacct
     3541 ttttcatgca gtacctgcgc cgccttcgct gcatcttcat cattttcaac acgaataccg
     3601 gtgagctttt ctgcttccgt ttcgtttggc gtaataatgt ccaccagtgc cagcagttcg
     3661 tcaggaagtt cgcgagccgg agccgggtta agcgcaacga tagtcttatt ttgatgggcg
     3721 attttcgccg ctgccatcac actttcgagt ggtgattcca gctgcattaa taatgctgac
     3781 gcgttggcaa tacgctcacg ttgcgcttcc accagtgccg gggaaagggc agcattagcg
     3841 ccggcatgaa taccgatgac attctcacct tcgccattaa caaaaatcag cgccacacct
     3901 gttgattcgc ctttgatcac gctgaccgga gaaatatcga tgttatcagt ggcgagctgc
     3961 tggcgaacgc tctcaccaat gctgtcatcg ccagtgcagg caataaacgc gatattcgca
     4021 ccgctacgcc cagcagccac agcctgatta gcgcctttgc cgccaaatgc aacctgatag
     4081 tggttaccgg ttacggtttc gcctggggta ggaaaagatt gaagattaag aatgtggtca
     4141 gcattaatgc tgccaagaac aacgaggctg cctgcgtttt gcatattcgg gatgtccata
     4201 aaatgcgcca ccgtgttagg gtggcgcgtg ccctgctttt ctttatatta cacgttcatc
     4261 tgtcgatgcc gtatttatgt caccatcagg tcatacaacc tgattaaaac tactgcttaa
     4321 caaccagttt cagatcaacc ggatacttag cctgaacttt ctcacctttc agtactttat
     4381 cggcggtttc gacgcctttc gcgccaatct gatcgggtag ctgagcgata gtcgctgcta
     4441 gtttgccatc attcaccgct ttttcgccat ccggtgtacc gtcaaatccg acgaccatca
     4501 catccgattt accggcagtt tgcagtgcgc gcagcgcgcc cagcgccatt tcatcattct
     4561 gcgcgaatac agcctgaaca tccggatgtg cggtcaacag gttctgcatt acgttcaaac
     4621 ctttagtgcg atcaaaatct gctggctggc tggcaagaac attaaacttg tgagcagcaa
     4681 cagcctgctg gaagccttcg ccacgttcac gggctgcgga tgtaccggca atgccttgca
     4741 gctcgataac tttggcacct tcacccgctt tcttcgcgat gtaatcaccg gcgattttgc
     4801 cgcccagtac gttatcagaa gcaatgtggc tcaccacttc acctttcgtt gcctggcggt
     4861 caagagtgat aaccgggatg ttcgcctggt tagccatctt cacagcatta cccactgcgt
     4921 cggagtcggt cgggttaatc agcagaattt tggtgccgcg aacggttaag tcctgcacgt
     4981 tcgccagctc tttcgccggg ttattctggg agtccagcac caccaggtta tagccaagtt
     5041 tatccgcctc tttctgcgcg ccatctttca gcgatacaaa gaacgggttg ttaagcgtgg
     5101 agaccaccag cgcgatggtg tcttttgcca tcgcattcgc actgacggtg gcgcttagcg
     5161 caacagcgga aaccagggta gccagttttt tcatgttcat attcaagatg tcctgtagtc
     5221 gttattactg ctttttgttg tctaccagca ccgccagcaa aatcaccacc gctttgacga
     5281 tcatctggta ataggaggaa acacctaaca aattcaatcc attattaagg aagccaagaa
     5341 ttaatgcgcc gatcaacgtc ccaacaatgc gaccttttcc gcccgccaga ctcgtaccgc
     5401 ccagaaccac cgcagcaatg gcatccagct catagccagt ccccgccgta ggttgtgcgg
     5461 aggagagacg cgccacttca atgatcccgg ccagcgatgc cagcagacca caaagagaat
     5521 agacgatgat tttgatttta ttgacgttga taccagaaag gcgcgttgcc gcttcgttgc
     5581 cgcccagcgc gtagatgtaa cgccccagac gcgtgtgatg cagcatgtac caggccgcga
     5641 ggaagacaat tcccatgatc cagactggcg tcggtacacc cagcggacga ccaataccaa
     5701 accagccaaa cagatcggcg ttctcagtaa agccagtatt cactgggcta ccgttggtat
     5761 aaaccatggt cacgccgcgc agtaaaagca tcataaccag cgtagcgata aaggcctgga
     5821 cgcgaccttt cgctacaatc accccggtta ccgcaccaat tgcggcacct aacgcgagag
     5881 cggcagcgac agccaccagc gcattgactt caatgccaac gatagatgca gcaactgcgc
     5941 cggtcagcgc caatagagaa cctaccgaca agtcgatgcc cgacgtcagg atcaccagcg
     6001 tcatcccgac tgccataatg gcgttcaccg aggtttgctg gagaatattg aataagttat
     6061 tgatggtgaa aaagttcggg cttaacgtcg agacaatcgc gatcagcacc agcagagcga
     6121 taagcgattt ctgctccatc agccacgctt tcgtgaaata acggcgacca gagacagtct
     6181 gggttgtcat ttttttactc ctgattcacg cgattaagct tgcccacagc ggcagccatt
     6241 aacacttcct gggtggcctg ctcacgagta aattccccgc tgagatgccc ttcatgcatg
     6301 acgatgatgc gatcgctcat gcctaatact tctggcatct ccgatgacac cagaatgatg
     6361 ctcaagccat cggctttgaa ctggttaatc agttgataga tctctttttt cgcgccgaca
     6421 tctacgccac gggtaggctc atcgaggatc aacactttgg ggcgtgtcat cagaccacgg
     6481 gcaatcgcca ctttttgctg attgccaccg gagagcagac caattgcctg ttccatcgac
     6541 ggcgttttca cattaaacag acgaatgaaa tcacttactg cctgctgttc atcggcatgc
     6601 ttcaaactgc cgccagcgcg gctgaagtag cgcagcgctg tcagcgacat gttctctttt
     6661 actgacatgc ccagcactaa accgtcacgt ttacggtctt cggagatata cacaatgccg
     6721 tttgccaggc catcctgcgg tgaacgggta acgacttcat gcccatccag ggtgacgtaa
     6781 ccgctggtgc gcggcagtgc gccgtagagc actttcatca gttcggtacg acctgcgccc
     6841 atcaaaccag agacgccaag aatttcgcct ttgcgtaaag taaaagagac atcgttaacg
     6901 ccaggtccgc agagattatc gactttcagg cggatatctc ccggcgcttt gtccaggtgc
     6961 ggatattgat cttccagctt gcgacccacc atcatctcaa tcagcgaatc ttcggtcagt
     7021 gatgctactt cacgctcagc aataaattgc ccatcacgaa aaacggtaac gtcatcgcaa
     7081 atctcgaaga tttctttcat gcggtgggag atatagacaa taccgcggcc ttgcgatttc
     7141 agctcgcgga tgacgcggaa caaggattcg gtttcggtat cggtcagcgc atcggtcggt
     7201 tcatccataa tgatgacttt cgactcaaag ctcagcactt tggcaatttc aaccatttgc
     7261 tggtcaccga tggaaagatc gcccaccagc ttgtcgcttt taaagcgcag gttaagttta
     7321 gccagcaatt tatccgcttc ggcatacatg gttttccagt caattttgcc aaagcgatta
     7381 acaaactcac gaccgaggaa aatgttttcg gcaatggtca actgcgggat caggttcagt
     7441 tcctgatgga taatcccaat cccggcttcc tgggaagatt tcggcccggt aaacgtcgtt
     7501 tctttcccca gccataaaag cgtaccggca tcgcgagtat agatacccgt aagcactttc
     7561 atcatggtgg atttacccgc accgttttcg cccaccagcg ccatcacgcg gccaggatag
     7621 acatttaacg ctgcgcccga gagggctttt acgcccggga aggctttatc gatgccttta
     7681 agctgaagta atgcttccat gacggcctca gaacgtcacg ccagcacaga gaatgatatt
     7741 cgcatacgga gaacattctc cgctgcgaat taccgcctga ctttctgcgg tttgttgttt
     7801 gaattgttca tgcgtggtgt aacgaatttc aatggtattt ccctggtgtt tttgcagctg
     7861 ctcaaggtga gtgagcaacg tttcgtggag atgcggatta tgttgtttga tctcttccgc
     7921 gataatggct gcctcgacct gcatttcatt tgtgacgacg cccagcacct gcataaaaga
     7981 aggtacgccc tgggttaatg ccatatcgat acgcgttgta cttttgggga tgggtaaacc
     8041 agcatcacac accaccagcg tatcggtatg tcccagacgg gagatcaccg atgaaatatc
     8101 agaattaaga acggtgcctt ttttcatttt tttctccatc agcgaaacgt ttcgctgacc
     8161 cgcagtttaa tcctgactga gttgggaaaa ccatcgtgac gttacggaaa tgtgatcaac
     8221 ctcgaaacgt tttacatggt aattaaccat gaaaacagaa acgccccctt aaggagaaag
     8281 gaggcgtctg gcgttagatt tcgacctgag tacccagttc gataaccctg tttggcggga
     8341 tttcaaattg atccggcgca cgcagcgcat tacgttgcag cagcaagtac agcttgccgc
     8401 gcagacgcaa ataccacggg cgtttgccga ggatcaacga ctcatgcgac ataaagaagg
     8461 aggtttccat catccggcaa cttaatcctt ccagaccgca gcggtggaaa acttcttcca
     8521 cgtttggcgt ttctcgccaa ccataacttg ccaccacgcg ccagaaagtg ggcgacagtt
     8581 gttcaatctg tacccgacgg acgttatgga catatggcgc gtcttcggtg cgcagagtta
     8641 acagaatcac ccgctcatgc aataccttgt tatgtttaag gttatgcatc agcgcaaagg
     8701 gaatgacgtt gattgcacgt gacatataca ccgcggtccc gggcacgcga acgggcggtg
     8761 atttctccag cgaagcaatc atcgcctcca gagagttacc atgttcatgc atccgccgca
     8821 gcaaacggaa acgttcgctc ttccaggtgg tcatcacgat aaacatcacc gtaccgaggc
     8881 tcaatggcaa ccagccgccg gagagcagtt tatcgaggtt agcggtgaac aatggaatat
     8941 cgacacaaag gaaagcaatc aggatcagcg caacaaaata cttattccag tgccagttct
     9001 gacgtgccac ggtagtcgag agaatagacg tcagcaccat ggttccggtc accgcaatcc
     9061 cgtacgccgc cgccaggttg ctggagtgct caaagctgac aatcacaatc acgaccgcga
     9121 catagagcat ccagttcaca aagggaatat agatttgccc tgactccatt tcggaggtgt
     9181 gaataatgcg catcggcgac aaatatccca gacgtaccgc ctgacgcgtc aatgagaaga
     9241 cgccagagat aacagcctgc gaggcaatta ccgtcgccag tgcggcgatg atcagcagcg
     9301 ggatcagcgc ccagtccggt gccaacagga agaacgggtt cttaatcgct tccgggttct
     9361 ttaacaacaa cgctccctga ccgaagtaat taagggtcaa ggaaggtaat acgacggtga
     9421 accacgccag gcgaataggg aacttaccaa agtgccccat atcagcatac agcgcctcga
     9481 cccccgtaat cgacagcacc actgccccta atgcaataaa agaaaccgtt ttgtattcaa
     9541 ggaagaaatg caccgcccac atcggattca gcgcatgcag cacttccggg ttagcaataa
     9601 tgctacgtaa ccccagtcct gccagaatca aaaaccaggt cagcatgatc ggcgcaaaca
     9661 gcttaccgac catagccgtg ccatgctttt gaatcataaa taataaagtg agaacgataa
     9721 ttgagagggg aactatccag gtatccagct gcggggcgac gatttccaga ccttcaatgg
     9781 cggacatcac cgaaatagcg ggtgttatga cgacttcacc atagaaaaag ctgccgccga
     9841 ttagccccat aatcaccagc attgatgtgg ttcgcgccga cgtattacgc ccggcaagcg
     9901 acatcaacgt caggatcccc ccttcaccgg cgttatctgc ccgcatcacg aaggtgagat
     9961 atttaataga aaccacaaag attagcagcc agaagatcag cgataaaaag ccaaacacgg
    10021 catcgcgctc aacgccaaaa ccaaactggc cggacaaaca ttcacgaagt gtatataacg
    10081 ggctggtacc aatatcgccg tagacaactc caatcgccgc gagggtaatc gcgggcaatg
    10141 attgcttatt atcagtgctc atagactagt ctttcgttga aatatgaaat gtgtgcttag
    10201 tcccttggcc cacaaaaaag cgcacagtat gcacgattaa cggcaaaatc gtactcctaa
    10261 atgcgggcac attaacctgg ctcaaagaaa aatacccccc ctttgagact attttagcca
    10321 ccagcaagaa acgtctatac tcgcaattta cgcagaactt ttgacgaaag gacgccactt
    10381 cattatggct caccctcatt tattagcgga aagaatttcc cgcctgagca gttcgttgga
    10441 aaaggggctt tatgaacgta gccacgccat ccgcttgtgt ttattagcgg cattaagtgg
    10501 tgaaagtgtg ttcctccttg gcccgccagg tattgccaaa agtttgatcg cccggcgctt
    10561 aaaattcgcc tttcagaatg cccgcgcgtt tgaatatctg atgactcgct tctccacgcc
    10621 ggaagaggtt tttggtcccc tttctattca ggcgctaaaa gatgaagggc gctatgaacg
    10681 tttaaccagc ggttacctgc cggaagcaga aatcgtcttt ctggatgaga tctggaaagc
    10741 gggtccggca attcttaata ccctgctcac cgccattaac gagcgccagt tccgcaacgg
    10801 tgcacacgta gaaaaaatcc cgatgcgcct gctggtggcg gcctccaacg agctgccgga
    10861 agcagacagc agtctggaag cgttatatga ccgcatgctg attcgtctgt ggttagataa
    10921 agtgcaggat aaagcgaatt tccgctccat gctgaccagt caacaggatg aaaacgacaa
    10981 tccggttcct gcctccttgc aggtcacaga tgaagaatat gaacgctggc agaaagagat
    11041 tggtgaaatt acgctgcccg atcatgtatt tgagctgatt tttatgctgc gccagcaact
    11101 ggataaatta ccggatgcgc cttatgtctc ggatcgtcgc tggaaaaaag cgattcgatt
    11161 attgcaggcc agcgcctttt ttagcggtcg cagtgctgtt gccccggttg atctcatttt
    11221 gctgaaagat tgcctgtggt atgacgcgca aagcctgaat ttgatacaac aacaaattga
    11281 tgtattgatg accggtcatg cctggcaaca gcaaggaatg ttgacccgcc tgggcgcgat
    11341 tgtgcaacgt cacctgcaac tccagcagca acaaagcgat aaaacagcct taacggtaat
    11401 tcgtctgggc ggcattttca gccgtcgtca gcagtatcaa cttcctgtta acgttactgc
    11461 ttccactctg actctgctgc tgcaaaaacc gttaaaactg catgatatgg aagtggttca
    11521 tatctccttt gagcgtaacg cgctggaaca gtggctgagt aaaggtggtg aaattcgcgg
    11581 caaactgaac ggtatcggct ttgcccagaa attgaatctg gaagtggata gcgcccaaca
    11641 tcttgttgta cgcgatgtaa gtttacaagg cagtacgctg gcacttcccg gttcatcggc
    11701 tgaaggtctg ccaggtgaaa taaaacaaca actggaagag cttgaaagcg actggcgcaa
    11761 gcaacacgct ttattcagcg aacagcaaaa atgtctgttt atccctggcg actggttagg
    11821 tcgcattgaa gccagcctac aggatgtcgg cgcccagatt cgccaggcac aacaatgcta
    11881 acgctggata cgcttaatgt gatgctggcc gtcagcgaag agggattgat cgaagagatg
    11941 atcatcgcgc tgctggcctc accgcagctg gcggtcttct ttgaaaaatt cccacgactg
    12001 aaggcggcaa tcactgatga tgttccccgc tggcgtgagg cgctgcgcag tcggctgaaa
    12061 gatgcccgag tcccgccgga actcaccgaa gaggtgatgt gctatcagca aagccagctc
    12121 ctctccacgc cgcagtttat tgtgcagcta ccacagatcc tggacttact gcatcgtctg
    12181 aattctccat gggcagaaca agcccgacag ttggttgatg ctaacagtac gatcacttca
    12241 gcgttacaca cgctttttct ccagcgctgg cgtttaagtc tgatcgtgca agcaacgacg
    12301 ctaaatcagc agctattaga agaagaacgc gaacaactgt tgagtgaagt tcaggaacgc
    12361 atgacgctga gcgggcaact tgaaccgatt ctcgcagata acaataccgc agctggtcgt
    12421 ctgtgggata tgagcgccgg tcagcttaaa cgcggcgact atcagttgat tgtgaaatac
    12481 ggtgaatttc ttaacgaaca gccggaactg aaacgcctgg cagaacagtt ggggcgttca
    12541 cgggaagcca aatcaatacc gcgcaacgat gcgcagatgg aaaccttccg caccatggtg
    12601 cgcgaaccgg cgacggttcc tgagcaggtt gatggtctgc aacaaagcga tgatatttta
    12661 cgtctcctgc cgccagaact ggcgacactg gggataacgg aactggagta tgagttttac
    12721 cgtcggctgg tggaaaaaca gttgctcacc tatcgcctgc acggtgagtc gtggcgtgaa
    12781 aaagtgatcg aacgcccggt ggtacataaa gattacgacg aacagccgcg cggaccgttt
    12841 attgtctgtg tggatacttc cggctcaatg ggcggcttta atgaacagtg tgcgaaagcg
    12901 ttctgcatgg ccttgatgcg cattgctctc gctgaaaacc ggcgctgcta tattatgtta
    12961 ttttccaccg agatcgtccg ttatgagctt tcaggcccac aaggcatcga acaagcaatc
    13021 cgttttttaa gccagcagtt tcgtggcggc accgatcttg ccagttgttt tcgcgccatt
    13081 atggaacgct tgcaaagcag ggaatggttt gatgccgatg cggtggtgat ttctgatttt
    13141 atcgcccagc ggttgcctga cgacgtgacg agtaaagtga aagagctaca gcgcgtacat
    13201 cagcatcgct ttcatgccgt ggcgatgtct gcacatggca aacccggcat catgcgcatt
    13261 ttcgatcata tctggcgctt tgataccggg atgcgaagcc gcctgctcag acgctggcgg
    13321 cgataaatta ttacagcaga gaagggacgc tctcgcgaac agctgccggc catacaccac
    13381 actgaacctg gccgatatgt ggcagttgca gcagcagcat ggtcaaacga gactgcccga
    13441 taccgccgcc gatggtctgc ggcatttcac cgcgcagcag cgcctgatgc cactccagct
    13501 ccaggcgatc ttcatcaccg gtcagcgcca gctgatgttt caacgtatcg gcatccacgc
    13561 ggatccccat ggaagaaagc tcaaacgcat cttccagtac cgggttccat accagaatat
    13621 cgccgttcag acccgcatgg cccagctctg acggggtgct ccagtcatca taatccggtg
    13681 cgcgcacgtc gtgacgatga ccatcactca acttgccgcc aatcccgacg aggaataccg
    13741 cgccaagatc tttcgcaatc gcccgctcac gccctttggc atcgagatcc ggataacgag
    13801 acagtaactc ctggctgtgt acgaagtgga tctgatccgg caggaacggt gccaggccaa
    13861 actcttcgct aaccgcagct tcggttgctt taattcccgc ccagatcgcc tctaccgtgc
    13921 ttttcagagt cgagaattga cgctcaccgt cgcccattac gcgttcccag tcccactggt
    13981 caacatagac cgagtgcaac ggagaaagac ggtcttcatc ggggcgaagg gctttcatgt
    14041 gcgtgtacag cccttcgccc gcgctgaagt cgtgttgccc taatgtctga cgtttccact
    14101 tcgccagtga atgaaccact tcgaactggg catcaggcag agctttcact tttacctgca
    14161 ccgctttttc acagcccgac aagttatcct gcgtgccatc ccccacacgg ctaagaatcg
    14221 gcgcctggac ttcgatcagc cccagacgtt cttccagttg acgggaaaag tgagatttca
    14281 cgaagctaat ttgacgttgt ttggcaatgt aagcggtttt cattttttat actcctgcgt
    14341 cctgttgctt atgattaagc aacaaaaacc gacacgcatg caataatcat tcaataaaaa
    14401 gcctaactca cttttgattc attaaaaaag aaggctaaaa tagaatgaat catcaatccg
    14461 cataagaaaa tcctatggaa aattatctaa tcgacaatct ggaccgtggc atcctggaag
    14521 cattaatggg caatgcgcgc accgcttacg ccgaactggc gaaacaattt ggcgtcagtc
    14581 cggggacgat tcacgttcga gtagagaaaa tgaagcaggc ggggatcatt accggggcgc
    14641 gtattgatgt cagcccgaag cagctcggtt atgacgtagg ctgctttatc ggcattatat
    14701 taaagagcgc caaagactat ccttccgcgc tggcgaaact ggaaagcctt gatgaagtca
    14761 ctgaagccta ttacaccacc ggccactaca gcatctttat aaaagtgatg tgccgttcga
    14821 tcgacgctct ccagcatgta cttatcaaca agatccaaac aattgatgaa attcagtcca
    14881 ccgagacatt gatcgtcctg caaaacccga tcatgcgtac catcaagccc tgatcggctt
    14941 ttttaatccc atacttttcc acaggtagat cccaacgcgt tcacagcgta caatacgcca
    15001 ctcttaataa aggtggcggt ttatggcaga tatcactctt atcagcggca gcaccctcgg
    15061 cggtgccgaa tatgtagcag aacacctggc tgaaaagctg gaagaggcgg gttttaccac
    15121 cgaaacgctg cacggtccgc tgttggaaga tttatctgcc tctgggatct ggctggttat
    15181 cagctcaacc cacggtgccg gagatattcc ggacaacctt tctcctttct atgaagcatt
    15241 gcaggaacag aagcccgatc tttctgcggt ccgctttggc gcaatcggta ttggtagtcg
    15301 tgaatatgac accttttgtg gggctatcga taaactcgag gcagaactca aaaattccgg
    15361 tgcaaaacag acaggcgaaa cactgaagat caacattctt gatcacgaca ttccggaaga
    15421 tccggcagaa gaatggctgg gatcgtggat taatttactc aaataagtat acagatcgtg
    15481 cgatctactg tggataactc tgtcaggaag cttggatcaa ccggtagtta tccaaagaac
    15541 aaccgttgtt cagtttttga gttgtgtata acccctcatt ctgatcccag cttatacggt
    15601 ccaggatcac cgatcattca cagttaatga tcctttccag gttgttgatc ttaaaagccg
    15661 gatccttgtt atccacaggg cagtgcgatc ctaataagag atcacaatag aacagatctc
    15721 taaataaata gatcttcttt ttaataccca ggatcccagg tctttctcaa gtcgacaaag
    15781 ttgagtagaa tccacggccc gggcttcaat ccattttcat accgcgttat gcgaggcaat
    15841 caccatgttt tatccggatc cttttgacgt catcatcatt ggcgggggtc atgcaggcac
    15901 cgaggccgcg atggccgcgg cgcgtatggg tcaacagact ctgcttttga cacacaatat
    15961 cgacactctg gggcagatga gctgcaaccc ggcgatcggc ggtattggga agggacatct
    16021 ggtaaaagaa gtggatgcac tcggcggtct gatggcgaaa gcgatcgatc aggcgggtat
    16081 ccagtttagg atactaaacg caagcaaggg accggcggtt cgcgctaccc gagctcaggc
    16141 ggatcgtgtg ctctaccgtc aggcggtacg tacggcgctg gagaaccaac cgaacctgat
    16201 gatcttccag caggcggttg aagatcttat tgtcgaaaac gatcgcgtgg tcggtgctgt
    16261 tacccaaatg ggactgaagt tccgtgccaa agccgtcgtg cttaccgttg ggacgttcct
    16321 cgacggtaaa attcatatcg gtctggataa ctacagcggt ggtcgtgctg gtgatccgcc
    16381 gtccattccg ctctctcgcc gtttgcgtga actgccgctg cgcgttggtc gtctgaaaac
    16441 cgggacaccg ccgcgtattg atgcgcgtac tatcgacttc agcgtgctgg cgcaacagca
    16501 tggcgataac ccaatgccgg tattctcgtt tatgggtaat gcgtcccagc atccacagca
    16561 ggtgccgtgt tatatcaccc ataccaacga gaaaacccat gatgtgatcc gcagtaacct
    16621 cgatcgtagc ccaatgtatg caggggtgat cgaaggtgtc ggcccacgct actgcccgtc
    16681 gatcgaagac aaagtcatgc gcttcgccga cagaaatcag catcagatct tccttgaacc
    16741 ggaagggctg acctcaaacg aaatttatcc gaacggtatc tccaccagcc tgccgttcga
    16801 tgtgcagatg caaatcgtcc gctccatgca ggggatggaa aacgcgaaga tcgtgcgtcc
    16861 gggttatgcc attgagtatg acttcttcga tccacgcgac ctgaaaccga cgctggagag
    16921 caagtttatc caggggctgt tctttgctgg tcagattaac ggcactaccg gttacgaaga
    16981 agccgctgcg caaggtttgc tggccggtct taacgctgcc cgtctgtctg ctgacaaaga
    17041 aggttgggct ccggcgcgtt ctcaggcgta tctcggcgta ctggttgatg acctgtgcac
    17101 tttaggaacc aaagaaccgt atcgtatgtt tacctcgcgc gcagaatatc gtctgatgct
    17161 gcgcgaagat aatgcggatc tgcgtttgac tgaaattggt cgtgaactgg gcctggtgga
    17221 tgacgaacgt tgggcgcgct ttaacgagaa acttgagaat atcgagcgtg agcgtcagcg
    17281 tctgaaatcg acctgggtaa ccccgtcggc ggaagctgca gccgaagtga atgctcacct
    17341 gactgcgcca ctttcccgtg aagccagtgg tgaagatctg ctgcgtcgtc cggaaatgac
    17401 ttatgaaaaa ttaaccacgc tgacgccgtt tgcccctgcg ttgacagacg aacaggcggc
    17461 ggaacaggtt gagattcagg ttaaatacga aggttatatc gcgcgccagc aagatgagat
    17521 cgaaaagcag ctgcgtaacg agaacaccct gctaccagcg acgctggatt accgccaggt
    17581 atccggtctt tctaacgaag tgatcgccaa acttaacgat cacaaaccgg cctctatcgg
    17641 ccaggcttcg cgtatttctg gcgtcacgcc tgcggccatc tccattctgc tggtgtggct
    17701 gaaaaaacag ggtatgctgc gtcgtagcgc ataacgcatt aaaaatgcct ggtaagcacc
    17761 cgcttaccgg gcaacgcatc aagaacaggt aatcaccgtg ctcaacaaac tctccttact
    17821 gctgaaagac gcaggtattt cgcttaccga tcaccagaaa aaccagctta ttgcctatgt
    17881 gaatatgctg cataaatgga ataaagcgta caacctgact tcggtccgcg atcctaatga
    17941 gatgctggta cgccatattc tcgatagcat tgtggtggca ccgtatctgc aaggtgaacg
    18001 gtttatcgat gtcggcaccg gtccaggact gcctggcatt ccactctcta tcgtgcgtcc
    18061 tgaagcccat ttcactctgt tggatagcct tggtaaacgc gtgcgtttcc ttcgtcaggt
    18121 gcaacatgag cttaaactgg agaatattga gccagtacag agcagggtag aagagtttcc
    18181 ttcagagccg ccatttgatg gcgtaattag ccgcgctttt gcctctctga acgatatggt
    18241 gagctggtgt caccatcttc ctggtgagca aggccgtttc tacgcgctga aaggccaaat
    18301 gccggaagat gaaatcgctt tgttgcccga agaatatcag gtcgaatcag tggttaaact
    18361 tcacgttccg gccctggatg gcgaacgtca tctggtggtg attaaagcaa ataaaattta
    18421 atttttatca aaaaaatcat aaaaaattga ccggttagac tgttaacaac aaccaggttt
    18481 tctactgata taactggtta catttaacgc cacgttcact cttttgcatc aacaagataa
    18541 cgtggctttt tttggtaagc agaaaataag tcataagtga aaatatcagt ctgctaaaaa
    18601 tcggcgctaa gaatcatcat tggctgttaa aacattatta aaaatgtcaa tgggtggttt
    18661 ttgttgtgta aatgtcattt attaaaacag tatctgtttt tagactgaaa tatcataaac
    18721 ttgcaaaggc atcatttgcc aagtaaataa atatgctgtg cgcgaacatg ctcaatatgt
    18781 gatctgaagc acgctttatc accagtgttt acgcgttatt tacagttttt catgatcgaa
    18841 cagggtttgc agaaaagtcg caattgtatg cactggaaaa atatttaaac atttattcac
    18901 cttttggcta cttattgttt gaaatcacgg gggcgcaccg tataatttga ccgctttttg
    18961 atgcttgact ctaagcctta aagaaagttt tatacgacac gcggcatacc tcgaagggag
    19021 caggagtgaa aaacgtgatg tctgtgtcgc tcgtgagtcg aaacgttgct cggaagcttc
    19081 tgctcgttca gttactggtg gtgatagcaa gtggattgct gttcagcctc aaagacccct
    19141 tctggggcgt ctctgcaata agcgggggcc tggcagtttt tctgcctaac gttttgttta
    19201 tgatatttgc ctggcgtcac caggcgcata caccagcgaa aggccgggtg gcctggacat
    19261 tcgcatttgg cgaagctttc aaagttctgg cgatgttggt gttactggtg gtggcgttgg
    19321 cggttttaaa ggcggtattc ttgccgctga tcgttacgtg ggttttggtg ctggtggttc
    19381 agatactggc accggctgta attaacaaca aagggtaaaa ggcatcatgg cttcagaaaa
    19441 tatgacgccg caggattaca taggacacca cctgaacaac cttcagctgg acctgcgtac
    19501 attctcgctg gtggatccac ataacccccc agccaccttc tggacaatca atattgactc
    19561 catgttcttc tcggtggtgc tgggtctgtt gttcctggtt ttattccgta gcgtagccaa
    19621 aaaggcgacc agcggtgtgc caggtaagtt tcagaccgcg attgagctgg tgatcggctt
    19681 tgttaatggt agcgtgaaag acatgtacca tggcaaaagc aagctgattg ctccgctggc
    19741 cctgacgatc ttcgtctggg tattcctgat gaacttgatg gatttactgc ctatcgacct
    19801 gctgccgtac attgctgaac atgtactggg tctgcctgca ctgcgtgtgg ttccgtctgc
    19861 ggacgtgaac gtaacgctgt ctatggcact gggtgtattt atcctgattc tgttctacag
    19921 catcaaaatg aaaggcatcg gcggcttcac gaaagagttg acgctgcagc cgttcaatca
    19981 ctgggcgttc attcctgtca acttaatcct tgaaggggta agcctgctgt ccaaaccagt
    20041 ttcactcggt ttgcgactgt tcggtaacat gtatgccggt gagctgattt tcattctgat
    20101 tgctggtctg ttgccgtggt ggtcacagtg gattctgaat gtgccgtggg ccattttcca
    20161 catcctgatc attacgctgc aagccttcat cttcatggtt ctgacgatcg tctatctgtc
    20221 gatggcgtct gaagaacatt aatttaccaa cactactacg ttttaactga aacaaactgg
    20281 agactgtcat ggaaaacctg aatatggatc tgctgtacat ggctgccgct gtgatgatgg
    20341 gtctggcggc aatcggtgct gcgatcggta tcggcatcct cgggggtaaa ttcctggaag
    20401 gcgcagcgcg tcaacctgat ctgattcctc tgctgcgtac tcagttcttt atcgttatgg
    20461 gtctggtgga tgctatcccg atgatcgctg taggtctggg tctgtacgtg atgttcgctg
    20521 tcgcgtagta agcgttgctt ttatttaaag agcaatatca gaacgttaac taaatagagg
    20581 cattgtgctg tgaatcttaa cgcaacaatc ctcggccagg ccatcgcgtt tgtcctgttc
    20641 gttctgttct gcatgaagta cgtatggccg ccattaatgg cagccatcga aaaacgtcaa
    20701 aaagaaattg ctgacggcct tgcttccgca gaacgagcac ataaggacct tgaccttgca
    20761 aaggccagcg cgaccgacca gctgaaaaaa gcgaaagcgg aagcccaggt aatcatcgag
    20821 caggctaaca aacgccgctc gcagattctg gacgaagcga aagctgaggc agaacaggaa
    20881 cgtactaaaa tcgtggccca ggcgcaggcg gaaattgaag ccgagcgtaa acgtgcccgt
    20941 gaagagctgc gtaagcaagt tgctatcctg gctgttgctg gcgccgagaa gatcatcgaa
    21001 cgttccgtgg atgaagctgc taacagcgac atcgtggata aacttgtcgc tgaactgtaa
    21061 ggagggaggg gctgatgtct gaatttatta cggtagctcg cccctacgcc aaagcagctt
    21121 ttgactttgc cgtcgaacac caaagtgtag aacgctggca ggacatgctg gcgtttgccg
    21181 ccgaggtaac caaaaacgaa caaatggcag agcttctctc tggcgcgctt gcgccagaaa
    21241 cgctcgccga gtcgtttatc gcagtttgtg gtgagcaact ggacgaaaac ggtcagaacc
    21301 tgattcgggt tatggctgaa aatggtcgtc ttaacgcgct cccggatgtt ctggagcagt
    21361 ttattcacct gcgtgccgtg agtgaggcta ccgctgaggt agacgtcatt tccgctgccg
    21421 cactgagtga acaacagctc gcgaaaattt ctgctgcgat ggaaaaacgt ctgtcacgca
    21481 aagttaagct gaattgcaaa atcgataagt ctgtaatggc aggcgttatc atccgagcgg
    21541 gtgatatggt cattgatggc agcgtacgcg gtcgtcttga gcgccttgca gacgtcttgc
    21601 agtcttaagg ggactggagc atgcaactga attccaccga aatcagcgaa ctgatcaagc
    21661 agcgcattgc tcagttcaat gttgtgagtg aagctcacaa cgaaggtact attgtttctg
    21721 taagtgacgg tgttatccgc attcacggcc tggccgattg tatgcagggt gaaatgatct
    21781 ccctgccggg taaccgttac gctatcgcac tgaacctcga gcgcgactct gtaggtgcgg
    21841 ttgttatggg tccgtacgct gaccttgccg aaggcatgaa agttaagtgt actggccgta
    21901 tcctggaagt tccggttggc cgtggcctgc tgggccgtgt ggttaacact ctgggtgcac
    21961 caatcgacgg taaaggtccg ctggatcacg acggcttctc tgctgtagaa gcaatcgctc
    22021 cgggcgttat cgaacgtcag tccgtagatc agccggtaca gaccggttat aaagccgttg
    22081 actccatgat cccaatcggt cgtggtcagc gtgaattgat catcggtgac cgtcagaccg
    22141 gtaaaaccgc actggctatc gatgccatca tcaaccagcg cgattccggt atcaaatgta
    22201 tctatgtcgc tatcggccag aaagcgtcca ccatttctaa cgtggtacgt aaactggaag
    22261 agcacggcgc actggctaac accatcgttg tggtagcaac cgcgtctgaa tccgctgcac
    22321 tgcaatacct ggcaccgtac gctggttgtg caatgggcga atacttccgt gaccgcggtg
    22381 aagatgcgct gatcatttac gatgacctgt ctaaacaggc tgttgcttac cgtcagatct
    22441 ccctgctgct ccgtcgtccg ccaggacgtg aagcattccc gggcgacgta ttctacctcc
    22501 actctcgtct gctggagcgt gctgcgcgtg ttaacgccga atacgttgaa gccttcacca
    22561 aaggtgaagt gaaagggaaa accggttctc tgactgcgct gccgattatc gaaactcagg
    22621 cgggtgacgt ttctgcgttc gttccaacca acgtaatctc cattaccgat ggtcagatct
    22681 tcctggaaac caacctgttc aacgccggta ttcgtcctgc ggttaacccg ggtatttccg
    22741 tatcccgtgt tggtggtgca gcacagacca agatcatgaa aaaactgtcc ggtggtatcc
    22801 gtaccgctct ggcacagtat cgtgaactgg cagcgttctc tcagtttgca tccgaccttg
    22861 acgatgcaac acgtaagcag cttgaccacg gtcagaaagt gaccgaactg ctgaagcaga
    22921 aacagtatgc gccgatgtct gttgcgcaac agtctctggt tctgttcgca gcagaacgtg
    22981 gttacctggc ggatgtagaa ctgtcgaaaa tcggcagctt cgaagccgct ctgctggctt
    23041 acgtcgaccg tgatcacgct ccgttgatgc aagagatcaa ccagaccggt ggctacaacg
    23101 acgaaatcga aggcaagctg aaaggcatcc tcgattcctt caaagcaacc caatcctggt
    23161 aacgtctggc ggcttgcctt agggcaggcc gcaaggcatt gaggagaagc tcatggccgg
    23221 cgcaaaagag atacgtagta agatcgcaag cgtccagaac acgcaaaaga tcactaaagc
    23281 gatggagatg gtcgccgctt ccaaaatgcg taaatcgcag gatcgcatgg cggccagccg
    23341 tccttatgca gaaaccatgc gcaaagtgat tggtcacctt gcacacggta atctggaata
    23401 taagcaccct tacctggaag accgcgacgt taaacgcgtg ggctacctgg tggtgtcgac
    23461 cgaccgtggt ttgtgcggtg gtttgaacat taacctgttc aaaaaactgc tggcggaaat
    23521 gaagacctgg accgacaaag gcgttcaatg cgacctcgca atgatcggct cgaaaggcgt
    23581 gtcgttcttc aactccgtgg gcggcaatgt tgttgcccag gtcaccggca tgggggataa
    23641 cccttccctg tccgaactga tcggtccggt aaaagtgatg ttgcaggcct acgacgaagg
    23701 ccgtctggac aagctttaca ttgtcagcaa caaatttatt aacaccatgt ctcaggttcc
    23761 gaccatcagc cagctgctgc cgttaccggc atcagatgat gatgatctga aacataaatc
    23821 ctgggattac ctgtacgaac ccgatccgaa ggcgttgctg gataccctgc tgcgtcgtta
    23881 tgtcgaatct caggtttatc agggcgtggt tgaaaacctg gccagcgagc aggccgcccg
    23941 tatggtggcg atgaaagccg cgaccgacaa tggcggcagc ctgattaaag agctgcagtt
    24001 ggtatacaac aaagctcgtc aggccagcat tactcaggaa ctcaccgaga tcgtctcggg
    24061 ggccgccgcg gtttaaacag gttatttcgt agaggattta agatggctac tggaaagatt
    24121 gtccaggtaa tcggcgccgt agttgacgtc gaattccctc aggatgccgt accgcgcgtg
    24181 tacgatgctc ttgaggtgca aaatggtaat gagcgtctgg tgctggaagt tcagcagcag
    24241 ctcggcggcg gtatcgtacg taccatcgca atgggttcct ccgacggtct gcgtcgcggt
    24301 ctggatgtaa aagacctcga acacccgatc gaagtcccgg taggtaaagc gactctgggc
    24361 cgtatcatga acgtactggg tgaaccggtc gacatgaaag gcgagatcgg tgaagaagag
    24421 cgttgggcga ttcaccgcgc agcaccttcc tacgaagagc tgtcaaactc tcaggaactg
    24481 ctggaaaccg gtatcaaagt tatcgacctg atgtgtccgt tcgctaaggg cggtaaagtt
    24541 ggtctgttcg gtggtgcggg tgtaggtaaa accgtaaaca tgatggagct tattcgtaac
    24601 atcgcgatcg agcactccgg ttactctgtg tttgcgggcg taggtgaacg tactcgtgag
    24661 ggtaacgact tctaccacga aatgaccgac tccaacgtta tcgacaaagt atccctggtg
    24721 tatggccaga tgaacgagcc gccgggaaac cgtctgcgcg ttgctctgac cggtctgacc
    24781 atggctgaga aattccgtga cgaaggtcgt gacgttctgc tgttcgttga caacatctat
    24841 cgttacaccc tggccggtac ggaagtatcc gcactgctgg gccgtatgcc ttcagcggta
    24901 ggttatcagc cgaccctggc ggaagagatg ggcgttctgc aggaacgtat cacctccacc
    24961 aaaaccggtt ctatcacctc cgtacaggcg gtatacgtac ctgcggatga cttgactgac
    25021 ccgtctccgg caaccacctt tgcgcacctt gacgcaaccg tggtactgag ccgtcagatc
    25081 gcgtctctgg gtatctaccc ggccgttgac ccgctggact ccaccagccg tcagctggac
    25141 ccgctggtgg ttggtcagga acactacgac accgcgcgtg gcgttcagtc catcctgcaa
    25201 cgttatcagg aactgaaaga cattatcgcc atcctgggta tggatgaact gtctgaagaa
    25261 gacaaactgg tggtagcgcg tgctcgtaag atccagcgct tcctgtccca gccgttcttc
    25321 gtggcagaag tattcaccgg ttctccgggt aaatacgtct ccctgaaaga caccatccgt
    25381 ggctttaaag gcatcatgga aggcgaatac gatcacctgc cggagcaggc gttctacatg
    25441 gtcggttcca tcgaagaagc tgtggaaaaa gccaaaaaac tttaacgcct taatcggagg
    25501 gtgatatggc aatgacttac cacctggacg tcgtcagtgc agagcaacaa atgttctctg
    25561 gtctggtcga gaaaatccag gtaacgggta gcgaaggtga actggggatc tatcctggcc
    25621 acgcaccgct gctcaccgcc attaagcctg gtatgattcg catcgtgaaa cagcacggtc
    25681 acgaagagtt tatctatctg tctggcggca ttcttgaagt gcagcctggc aacgtgaccg
    25741 ttctggccga caccgcaatt cgcggccagg atctcgacga agcgcgagcc atggaagcga
    25801 aacgtaaggc tgaagagcac attagcagct ctcacggcga cgtagattac gctcaggcgt
    25861 ctgcggaact ggccaaagcg atcgcgcagc tgcgcgttat cgagttgacc aaaaaagcga
    25921 tgtaacaccg gcttgaaaag cacaaaagcc agtctggaaa caggctggct ttttttacgc
    25981 gtgacccgtc ctgaagaacg ttcacataga tcctgctgat ataaaacccc ctgttttccc
    26041 gtttattcat tgatcgaaat aagagcaaaa acatccacct gacgcttaaa tgaaggtact
    26101 gccttaattt tctgcagaca aaaggtgtga cgatggtcga aaatggcgct ttcgtcagcg
    26161 gggataatcc gttattgaac aatttatcct ctgtccattt cacgatgaaa aaaatgtagt
    26221 tttttcaagg tgaagcggtt taaattcgtt ctcaaattac agtcaggacg cgtatgttga
    26281 ataatgctat gagcgtagtg atccttgccg caggcaaagg cacgcgcatg tattccgatc
    26341 ttccgaaagt gctgcatacc cttgccggga aagcgatggt tcagcatgtc attgatgctg
    26401 cgaatgaatt aggcgcagcg cacgttcacc tggtttacgg tcacggcggc gatctgctta
    26461 aacaggcgct gaaagatgac aacctgaact gggtgcttca ggcagaacag ctgggtacag
    26521 gtcatgcgat gcagcaggcc gcacctttct ttgccgatga tgaagacatt ttaatgctct
    26581 acggcgacgt gccgctgatc tctgtcgaaa cactccagcg tctgcgtgat gctaaaccgc
    26641 agggtggcat tggtctgctg acggtaaaac tggatgatcc gaccggttat ggacgtatca
    26701 cccgtgaaaa cggcaaagtt accggcattg ttgagcacaa agacgccacc gacgagcagc
    26761 gtcagattca ggagatcaac accggcattc tgattgccaa cggcgcagat atgaaacgct
    26821 ggctggcgaa gctgaccaac aataacgctc agggtgaata ctacatcacc gacattattg
    26881 cgctggcgta tcaggaaggg cgtgaaatcg tcgccgttca tccgcaacgt ttaagcgaag
    26941 tagaaggcgt gaataaccgc ctgcaactct cccgactgga gcgcgtttac cagtctgaac
    27001 aggctgaaaa actgctgtta gcaggcgtta tgctgcgcga tccggcgcgt tttgatctgc
    27061 gcggtacgct tactcacggg cgcgatgttg aaattgatac taacgttatc atcgagggca
    27121 acgtgactct cggtcatcgc gtgaaaatcg gcaccggttg cgtgattaaa aacagcgtga
    27181 ttggcgatga ttgcgaaatc agtccgtata ccgttgtgga agatgcgaat ctggcagcgg
    27241 cctgtaccat tggcccgttt gcccgtctgc gtcctggtgc tgagttgctg gaaggtgcac
    27301 acgtcggtaa cttcgttgag atgaaaaaag cgcgtctggg taaaggctcg aaagccgggc
    27361 atctgactta tctgggcgat gcggaaattg gcgataacgt taacatcggc gcgggaacca
    27421 ttacctgcaa ctacgacggt gcgaataaat ttaagaccat tatcggcgac gatgtgtttg
    27481 ttggttccga tactcagctg gtggccccgg taacggtagg caaaggcgca accattgctg
    27541 cgggtacaac tgtgacgcgt aatgtcggcg aaaatgctct ggcaatcagc cgtgtgccgc
    27601 agtcgcaaaa agaaggctgg cgtcgtccgg taaagaaaaa gtaattctgg ctgggaaatc
    27661 cggtcatatg ggatgaggag ataacataat ctcccgccca caagcagtaa ctataaaaat
    27721 aaccccactc tctacaaggc tcggggcgcc cgaaaaaacg ggcatacagg ttgaccgaca
    27781 acgatataaa tcggaatcaa aaactatgtg tggaattgtt ggcgcgatcg cgcaacgtga
    27841 tgtagcagaa atccttcttg aaggtttacg tcgtctggaa taccgcggat atgactctgc
    27901 cggtctggcc gttgttgatg cagaaggtca tatgacccgc ctgcgtcgcc tcggtaaagt
    27961 acagatgctg gctcaggcag cggaagaaca tcctctgcat ggcggcaccg gtattgctca
    28021 tactcgctgg gcgacccacg gtgaaccttc agaagctaac gcgcatccgc atgtttctga
    28081 acacattgtg gtggtgcata acggcatcat cgaaaaccat gaaccgctgc gtgaagagtt
    28141 aaaagcgcgt ggctatacct tcgtttctga aaccgacacc gaagtgattg cacatttggt
    28201 gaactgggag ctgaaacaag gcggtactct gcgtgaggca gttctgcgtg ctattccgca
    28261 actgcgtggt gcgtacggta cagtgatcat ggactcccgt cacccggata ccctgctggc
    28321 ggcacgttct ggtagcccgc tggtgattgg tctgggcatg ggcgaaaact ttatcgcttc
    28381 tgaccagctg gcgctgttgc cagtgactcg tcgctttatc ttccttgaag agggcgatat
    28441 tgcggaaatc actcgccgtt cggtaaacat tttcgataaa actggcgcgg aagtaaaacg
    28501 tcaggatatt gaatccaatc tgcaatatga cgcgggcgat aaaggtattt accgtcacta
    28561 catgcagaaa gagatctacg aacaaccgaa cgcgatcaaa aacaccctta ccgggcgcat
    28621 cagccacggt caggttgatt taagcgagct gggaccgaac gccgacgaac tgctgtcgaa
    28681 ggttgagcat attcagatcc tcgcctgtgg tacttcttat aactccggta tggtttcccg
    28741 ctactggttt gaatcgctgg caggtattcc gtgcgacgtc gaaatcgcct ctgaattccg
    28801 ctatcgcaaa tctgccgtgc gtcgtaacag cctgatgatc accttgtcac agtctggcga
    28861 aaccgcggat accctggcgg gcctgcgtct gtcgaaagag ctgggttacc ttggttcgct
    28921 ggcaatctgt aacgttccgg gttcttctct ggtgcgcgaa tccgatctgg cgctgatgac
    28981 caacgccggt acggaaatcg gcgtggcatc cactaaagca ttcaccactc agttaactgt
    29041 gctgttgatg ctggtggcga agctgtctcg cctgaaaggt ctggatgcct ccattgaaca
    29101 tgacattgtg catggtctgc aggcgctgcc gagccgtatt gagcagatgc tgtctcagga
    29161 caaacgcatt gaagcgctgg cagaagattt ctctgacaaa catcacgcgc tgttcctggg
    29221 ccgtggcgat cagtacccaa tcgcgctgga aggtgcattg aagctgaaag agatctctta
    29281 cattcacgct gaagcctacg ctgcgggcga actgaaacac ggcccgctgg cgctgattga
    29341 cgccgatatg ccggttatcg tcgttgcgcc gaacaacgag ttgttggaaa aactgaaatc
    29401 taacattgaa gaagttcgcg cgcgtggcgg tcagttgtat gtcttcgccg atcaggatgc
    29461 gggttttgtg agcagtgaca acatgcacat cattgagatg ccgcatgtgg aagaggtgat
    29521 tgcaccaatc ttctacaccg ttccgctgca gctactggct taccacgttg cgctgatcaa
    29581 aggcaccgac gttgaccagc cgcgtaacct ggcgaaatcg gttacggttg agtaatatct
    29641 ctcgttgata gaaaagggct gccgaccggc ggcccttttt tataa
//
LOCUS       DAZGQL010000068        28958 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.068, whole genome shotgun sequence.
ACCESSION   DAZGQL010000068 DAZGQL010000000
VERSION     DAZGQL010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28958
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.068"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..602)
                     /locus_tag="ACHKIR_003259"
     CDS             complement(<1..602)
                     /locus_tag="ACHKIR_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFO9990946.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDE"
     gene            complement(813..2210)
                     /gene="ccp"
                     /locus_tag="ACHKIR_003260"
     CDS             complement(813..2210)
                     /gene="ccp"
                     /locus_tag="ACHKIR_003260"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HFO9990947.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LANKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            2615..4264
                     /locus_tag="ACHKIR_003261"
     CDS             2615..4264
                     /locus_tag="ACHKIR_003261"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005993 - trehalose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFO9990948.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKACARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKLI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
     gene            complement(4315..4917)
                     /locus_tag="ACHKIR_003262"
     CDS             complement(4315..4917)
                     /locus_tag="ACHKIR_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFO9990949.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
     gene            5437..6336
                     /locus_tag="ACHKIR_003263"
     CDS             5437..6336
                     /locus_tag="ACHKIR_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO9990950.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPPGI
                     AKSLLLPRLSEFLYLHPGIELELSSHDRPVDILHDGFDCVIRTGALPEDGVIARSLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSRENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            6385..7398
                     /gene="yhjD"
                     /locus_tag="ACHKIR_003264"
     CDS             6385..7398
                     /gene="yhjD"
                     /locus_tag="ACHKIR_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HFO9990951.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            7791..9113
                     /locus_tag="ACHKIR_003265"
     CDS             7791..9113
                     /locus_tag="ACHKIR_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9990952.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVASI
                     LGASVAPYIAAWLQTNYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(9292..11352)
                     /locus_tag="ACHKIR_003266"
     CDS             complement(9292..11352)
                     /locus_tag="ACHKIR_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFO9990953.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLASDDNKDTNAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLDIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(11422..12189)
                     /gene="pdeH"
                     /locus_tag="ACHKIR_003267"
     CDS             complement(11422..12189)
                     /gene="pdeH"
                     /locus_tag="ACHKIR_003267"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HFO9990954.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFIEHDLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPIETLNTAVLAL"
     gene            12421..13350
                     /gene="kdgK"
                     /locus_tag="ACHKIR_003268"
     CDS             12421..13350
                     /gene="kdgK"
                     /locus_tag="ACHKIR_003268"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HFO9990955.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSAAICEELANFDYLYLSGISLAILSQTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAEDAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            complement(13446..14942)
                     /locus_tag="ACHKIR_003269"
     CDS             complement(13446..14942)
                     /locus_tag="ACHKIR_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFO9990956.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLEAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAAATSVATDDVHPEVTDIPPVQ"
     gene            complement(15163..16449)
                     /gene="dctA"
                     /locus_tag="ACHKIR_003270"
     CDS             complement(15163..16449)
                     /gene="dctA"
                     /locus_tag="ACHKIR_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HFO9990957.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            complement(16632..18620)
                     /gene="hmsP"
                     /locus_tag="ACHKIR_003271"
     CDS             complement(16632..18620)
                     /gene="hmsP"
                     /locus_tag="ACHKIR_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HFO9990958.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQQRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            complement(18702..22193)
                     /gene="bcsC"
                     /locus_tag="ACHKIR_003272"
     CDS             complement(18702..22193)
                     /gene="bcsC"
                     /locus_tag="ACHKIR_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex outer membrane
                     protein BcsC"
                     /protein_id="HFO9990959.1"
                     /translation="MRKFTLNIFTLSLGLAVMPMVEAAPTAQQQLLEQVRLGEATHRE
                     DLVQQSLYRLELIDPNNPDVVAARFRSLLRQGDIDGAQKQLDRLSQLAPSSNAYKSSR
                     TTMLLSTSDGRQALQQARLQATTGHAEEAVASYNKLFNGAPPEGDIAVEYWSTVAKIP
                     ARRGEAINQLKRINADTPGNTGLQNNLALLLFSSDRRDEGFAVLEQMAKSNAGREGAS
                     KIWYGQIKDMPVSDASVQALKKYLSIFSDGDSVAAAQSQLAEQQKQLADPAFRARAQG
                     LAAVDSGMAGKAIPELQQAVRANPKDSEALGALGQAYSQKGDRANAVANLEKALALDP
                     HNSNNDKWNSLLKVNRYWLAIQQGDAALKANNPDRAERLFQQARNVDNTDSYAVLGLG
                     DVAMARKDYPAAERYYQQTLRMDSGNTNAVRGLANIYRQQSPEKAEAYIASLSASQRR
                     SIDDIERSLQNDRLAQQAEALENQGKWAQAAALQRQRLALDPGSVWITYRLSQDLWQA
                     GQRSQAGQRSQADTLMRNLAQQKPNDPEQVYAYGLYLSGHDQDRAALAHINSLPRAQW
                     SSNIQELVNRLQSDQVLETANRLRESGKEAEAEAMLRQQPPSTRIDLTLADWAQQRRD
                     YTAARAAYQNVLTREPTNADAILGLTEVDIAAGDKAAARSQLAKLPATDNASLNTQRR
                     VALAQAQLGDTAAAQQTFNKLIPLAKSQPPSMESAMVLRDGAKFEAQAGDPTQALETY
                     KDAMVASGVTTTRPLDNDTFTRLTRNDEKDDWLKRGVRSDAADLYRQQDLNVTLEHDY
                     WGSSGTGGYSDLKAHTTMLQVDAPYSDGRMFFRSDFVNMNVGSFSTNADGKWDDNWGT
                     CTLQDCSGNRSQSDSGASVAVGWRNDTWSWDIGTTPMGFNVVDVVGGISYSDDIGPLG
                     YTVNAHRRPISSSLLAFGGQKDSPSNTGKKWGGVRADGVGLSLSYDKGEANGVWASLS
                     GDQLTGKNVEDNWRVRWMTGYYYKVINQNNRRVTIGLNNMIWHYDKDLSGYSLGQGGY
                     YSPQEYLSFAIPVMWRERTENWSWELGASGSWSHSRTKTMPRYPLMNLIPTDWQEEAA
                     RQSNDGGSSQGFGYTARALLERRVTSNWFVGTAIDIQQAKDYAPSHFLLYVRYSAAGW
                     QGDMDLPPQPLIPYADW"
     gene            complement(22175..23281)
                     /gene="bcsZ"
                     /locus_tag="ACHKIR_003273"
     CDS             complement(22175..23281)
                     /gene="bcsZ"
                     /locus_tag="ACHKIR_003273"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex periplasmic
                     endoglucanase BcsZ"
                     /protein_id="HFO9990960.1"
                     /translation="MNVLRSGLVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNLLDWTQNNLAQGSLKEHLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWALLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            complement(23288..25627)
                     /gene="bcsB"
                     /locus_tag="ACHKIR_003274"
     CDS             complement(23288..25627)
                     /gene="bcsB"
                     /locus_tag="ACHKIR_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HFO9990961.1"
                     /translation="MKRKIFWICAVALGMSAFPSFMTQATPATQPLINAEPAVAAQAE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIVGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAAISVVLLAWVLWRLLRIISRRRLNPDNE"
     gene            complement(25638..28256)
                     /gene="bcsA"
                     /locus_tag="ACHKIR_003275"
     CDS             complement(25638..28256)
                     /gene="bcsA"
                     /locus_tag="ACHKIR_003275"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016760 - cellulose synthase (UDP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HFO9990962.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYPHINASRPRPLDPVRYLIQTCWLLIGASRKETPKPR
                     RRAFSGLQNIRGRYHQWMNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTFSL
                     ILALICVTQPFNPLAQFIFLMLLWGVALIVRRMPGRFSALMLIVLSLTVSCRYIWWRY
                     TSTLNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVD
                     IFVPTYNEDLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIART
                     THEHAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTMGWFLKEKQLAMMQTPHHF
                     FSPDPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSCAVIRRKPLDEIGGIA
                     VETVTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLD
                     NPLTGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLFHAYIIYAPALMIALFV
                     LPHMIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGG
                     LVEEEYVDWVISRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLIIL
                     GGAVAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQIL
                     EGQKVNLLLKRGQQEYVFPAQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWAL
                     WQDSYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFIPRRP
                     ERSETAQPSDQALAQQ"
     gene            complement(28253..>28958)
                     /gene="bcsQ"
                     /locus_tag="ACHKIR_003276"
     CDS             complement(28253..>28958)
                     /gene="bcsQ"
                     /locus_tag="ACHKIR_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HFO9990963.1"
                     /translation="TITAALAWSLQMLGENVLVVDACPDNLLRLSFNVDFTHRQGWAR
                     AMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRLSDICTGLQQLKASGRY
                     QWILIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRLHQQALPDGAHILINDFRIG
                     SQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEYRSDALAAEEILTLANW
                     CLLNYSGLKTPVGSAS"
BASE COUNT         6829 a         7968 c         7281 g         6880 t
ORIGIN      
        1 ttttcgtcgc aggcttcaat catgcgtttc gggtccataa acaactgacc ggggcgcata
       61 gggatctcac gcaactccac atcccagtag cgggcgaatt tatgccagca gatttgtacc
      121 ggaccgcaca ccaggtttgg tttatccgtt ggtttgcctg cggcttccat acgcttgcgc
      181 caacgccatt tcatcgccat cccgccgagc atacaggcct cggaagaacc aatggtgttg
      241 gtgccaacgg cctgaccatt tttcggtgca ggcgcatgcc acagatcggc aaccatgttt
      301 acgcaacgca ggtcgattgc agcggattgc ggatattctt ctttgtcgat ccagttttta
      361 ttgatcgaca aatccatcaa tttatggacg ttttcgtcgt cccaggtctg gcagaaagtg
      421 gccaggttct gacgagcgtt gccatcaaga tataattcat cattgataat ctgaaatgca
      481 acatcatcgc gcatttcgtg cagcggaaat cgttttgact ccgcgatagt agaaatggcc
      541 tttgcaccaa aacgtgaatc gagtagttct gagcggaaat ccgttaacag cttctggtcc
      601 atttcgaact ccttaaattt atttgaaggc aataaaaaag taggatttat ccgcaatgga
      661 agcaaggcat tacaggctta atttaaataa caaaatccta agcagaaaaa cgacctttta
      721 taatataaac atttaatatg ataatattaa aaacagactt tattatcgta ataaaaatcg
      781 cccaaacact cctgttcggg cgatcaaatt aattattgtt tatcctgcat atacggcgtg
      841 taaaccccgt tcagactgtg caggaaagct acgatatcat ccacatcctc ctgcggcagc
      901 tctttgccta cctgatagcg cagcatcagt ttcaccgccc cgtccagcgt cggcacgtca
      961 ccgcgatgga agtacggtgc cgttaaagca acgttacgta aaccgggtac tttctgacgc
     1021 aatttatcac gctcttcttt agtaacattc atacgaccaa tatccgccgc cgtaatttca
     1081 ccaaagttaa agtctttttt cagccccaac ggttcaaagg aacgtccgcc gagaataata
     1141 ccaccatgac aagttgcaca tttattatct ttaaataatt gatagccttt tttctgttgc
     1201 gccgtcagag cattctcatc tccacgcaac catttatcaa atggggaatc cggcgtaatt
     1261 aatgttttct caaattcagc aatggcatca gtaatatttt cgccactgaa accttgtgga
     1321 tagacttcga ggaactgcgc tttaagctgc ggatcttttt ccagcttagc aataatttcg
     1381 tcccaggatt tcgacgccat ttcaatcggg ttcaacggcg gtccaccagc ctgatcctgc
     1441 aatgttgccg cacgaccatc ccagaactgc tcaacgttaa atactgagtt aaataccgtc
     1501 ggcgcgttaa tcggcccaac tgcgccacca acaccaatcg atgtttttct gccatcgacg
     1561 ccccccgcat tcaacgcatg gcaatgagcg catgaaatgg tgctatcagc cgataaacgg
     1621 ggatcgtgat acagcgcaaa acccaacgcc actttttgcg catcggtagg cagtttttgc
     1681 gggatgggct gcaccggttc attgcgatgc tccggagcag tatcattgct ggcgtaatat
     1741 tccgcgcgct gttttgcaat ccaggccagt atttccgccc gctcttcatc actcacctta
     1801 cccgcccagt gtagcgcggt ataacgcgtt ggcggcatag tttcatactg catcacccat
     1861 tcaatcttat tcagatcgct ttgcgaaacg ggtttattag ccagcagagc cgcacgcacg
     1921 gcctcgaggt taaaagattt atatccaagc tttatgtcgt aatccatcaa ctgtttcgcg
     1981 ccgggaatat aataataggc gggtaattct gccgaaggtg tatggcaata gtcgcatcct
     2041 ttttcacgga gaaagcctaa aaccttatta ttttcactga cagcagatgc ctgaacatcg
     2101 gcctgtttac tgcgtttatt atcgtggtac cacacataac cagataaccc taaatagcaa
     2161 atcgcgacgc cagccaggcc gatcgcggta atacgtgaga ccattttcat tattcttccc
     2221 tcacggtggt gagttattgt gacccagaaa attttccaga atgctgcaaa cgtctctgcg
     2281 agacaaagcc atcataaaaa acagggagat atgctttttg ataggaatca atcaattaca
     2341 tgaatataaa ctatcaataa gatagccagt gcctattacc cagatcagga atggcatatc
     2401 atgattgcgg cgagagcagg attgaatgtt ctgaaaatga aaaacaacac gaaatccgta
     2461 tttttataaa ttccattgat attagtgcgt tatatttttt ggcaggttaa taaacgtgat
     2521 ccaccgcacg ctttgtcgcc caccaggcgg agcgaatgac tacccttaaa gaaaagcccg
     2581 ataattagcg acgaatttcg gaggtcggat ccttatgctc aatcagaaaa ttcaaaaccc
     2641 taatccagac gaactgatga tcgaggtcga tctctgctat gaactggacc catatgaatt
     2701 aaaactggat gagatgatcg aggcagaacc ggaacccgag atgattgaag ggctgcccgc
     2761 ctctgatgcg ctgacgcctg ccgatcgcta tctcgaactg ttcgaacatg ttcagtcggc
     2821 gaaaattttc cccgacagta aaacctttcc cgactgcgca cctaaaatgg acccgctgga
     2881 tatcttaatc cgctaccgta aagtgcgccg tcatcgtgat tttgacttgc gcaagtttgt
     2941 tgaaaaccac ttctggctgc cggaggtcta ctccagcgag tatgtatcgg acccgcaaaa
     3001 ttccctgaaa gagcatatcg accagctgtg gccggtgcta acccgcgaac cgcaggatca
     3061 cattccgtgg tcttctctgc tggcgctgcc gcagtcatat attgtcccgg gcggccgttt
     3121 tagcgaaacc tactattggg attcctattt caccatgctg gggctggcgg aaagtggtcg
     3181 ggaagatttg ctgaaatgca tggccgataa cttcgcctgg atgatcgaaa actacggtca
     3241 catccccaac ggcaaccgca cctattattt gagccgctcg caaccaccgg tttttgcgct
     3301 gatggtggag ttgtttgaag aagatggtgt acgcggtgcg cgccgctatc tcgaccacct
     3361 gaaaatggaa tatgccttct ggatggacgg tgcagaatcg ttgatcccta atcaggccta
     3421 tcgccatgtt gtgcggatgc cggacggatc gctgctcaac cgttactggg acgatcgcga
     3481 cacaccgcgt gacgaatcct ggcttgagga cgttgaaacc gcgaaacatt ctggtcgccc
     3541 gcccaacgag gtgtaccgcg atttacgcgc gggggcggcc tccggttggg attactcttc
     3601 ccgctggctg cgtgatactg gtcgtctggc gagcattcgt accacccagt tcatccccat
     3661 cgatctgaat gccttcctgt ttaaactgga gagcgccatc gccaacatct cggcgctgaa
     3721 aggcgagaaa gagacagaag cactgttccg ccagaaggcc tgtgcccgtc gcgatgcggt
     3781 aaaccgttac ctctgggatg atgaaaacgg tatctaccgc gattacgact ggcgacgcga
     3841 gcaactggcg ctgttttccg ctgccgccat tgtgccgctc tatgtcggta tggcgaacca
     3901 tgaacaggcc gatcgtctgg caaacgccgt gcgcagccga ttactgacac ctggcgggat
     3961 tctggcaagc gagtacgaaa ccggtgaaca gtgggataaa cccaacggct gggcaccgtt
     4021 acaatggatg gcgattcagg gatttaaaat gtatggcgat gaccttctgg gtgatgaaat
     4081 cgcgcgcagc tggctgaaga cggtgaatca gttctatctg gaacagcaca aactgatcga
     4141 gaaataccat attgccgatg gtgttccccg cgaaggcggc ggtggcgagt atccgttgca
     4201 ggatgggttt ggctggacta acggtgtggt gcgccgttta attggtttgt acggtgaacc
     4261 ataatatttt tacagccagc cgctaacttc ctgctggctg taaaattatc ctcttcagga
     4321 ggagatattt aacatcattg ccgcctgggt gcgatttttc acttccagac ggcgatacag
     4381 ggattccaga tgcgctttta ccgttccggt actgatattc agcgccctgc cgatctcctt
     4441 atttgattcg cccgccgcta acatggttaa aatctcccgc tggcgggcgc ttaacgattt
     4501 gagatcttta atgtcctttt ccggcgtcgt ccgccagtct ccaggcagaa acatcatccc
     4561 catcgccgca ctatttaccg ccaacgcaaa tgtctcgacg gttgaatcac gaggcacaat
     4621 ggccagcaca ttaaaatgga taacttcctg taaccaccgt ttattgcaat ccgtcgccgt
     4681 aattaacacc ttaacctcag gaaattgcac cacggttttt tgcagcaacc agtaacaaaa
     4741 ctcaccatcc tgatcgccat cgagcatcac taaggcttca gggtaacttt ccagcttttg
     4801 ccataactcg tctgcctgac tggccccctg aatactcact cctggaatac gttgctgtaa
     4861 actgattttc attccatgaa taaatattga ctgcctgtca aacatgacta tttgcataac
     4921 tgaatctcca cctgaatacg ttaaaaagac ttaagtagtg gaagggtatt acccgcgaga
     4981 aaaaataaga attcgccatt tggcggtggc cattctacag agatgacgcg tagaaaatag
     5041 ttaccgatat aaatagttac agctaaacgc ctgaaattac atgtcgaggg cactatttaa
     5101 aacaattttg aggatttcct tatattagtg gttagtacgc atgcaattaa aaatgaaatt
     5161 ccgcgaccac aagccaaaat aacaaatggc aatgagacaa aaataagcac aaatagccaa
     5221 tacgtcctct attcacttta aagggaatcg ctgaaaaata cgctctgttt aaggggattc
     5281 acctttctaa gaaagctatt cctccctttt cctactgaga aatcgccaca ttcgtcatga
     5341 caacattgtg aaacccggct ttagatgtta gaaaaaacaa taaacaatgc gatatgcgcg
     5401 ttactgtttc gctgtgaaca acaatcggtc aaagaaatgg ataaaattca cgcaatgcag
     5461 ttgttcatca aagtcgcgga gctggaaagt ttttcccgcg cggcggattt ctttgctttg
     5521 ccaaagggaa gtgtttcgcg ccagatacag gcactggaac atcaacttgg cacccagctt
     5581 ctccagcgca ctacgcgacg ggtcaaactc acgccagaag gcatgaccta ttatcaacgg
     5641 gcaaaagatg tgttgagtaa tctcagcgaa ctggacggtc tgtttcaaca ggatgccacc
     5701 agtatcagcg gtaaattacg catcgacatc ccgccaggaa tcgcgaaaag cctgttactg
     5761 ccgcgcttgt cggaatttct ctatctgcat ccgggaattg agctggaact gagtagccat
     5821 gaccgtccgg tagatattct tcatgatggt tttgattgcg tgatacgcac tggcgcatta
     5881 ccggaagatg gcgttatcgc ccgttctctc ggcaaactga ccatggtcaa ctgtgccagt
     5941 ccacactatc tgacgcgctt tggttatccg caaagccccg atgatctgac ttcacacgca
     6001 atagtgcgtt atacaccgca cctgggtgta catccgttag gttttgaggt tgccagcgtt
     6061 aatggcgtcc agtggtttaa gtctggcggc atgttgacgg taaacagtcg cgaaaactat
     6121 ctcgccgccg gtcttgccgg tctggggatt attcagatcc cgcgcattgc cgtgcgcgaa
     6181 gccctgcgtg ccgggcggct tattgaagtg ttgcctggct accgtgccga gccgctctcc
     6241 ctttcactgg tttatccgca gcgtcgggag ctttcccggc gtgtaaacct gtttatgcag
     6301 tggctggctg gcgtaatgaa agagcacctg gactgaccga ctatactttt taagaatgac
     6361 cacgacagaa ggacaaaaga gcggatgacg caggaaaacg agatcaaacg tcccacccag
     6421 gatctggagc acgagccgat taagcagttg gataatagcg agaaaggcgg caaagttagc
     6481 caggcgctgg aaaccgtcac cactactgcc gagaaagtcc aacgccaacc ggttattgcg
     6541 cacctgattc gcgcaacaga gcgctttaac gatcggctgg gtaaccagtt tggtgcggct
     6601 atcacctatt tctcattttt gtcgatgatc ccgattttga tggtatcgtt tgccgcaggg
     6661 ggctttgtgc tggcctccca tccgatgttg ctacaggata tcttcgacaa aattctgcaa
     6721 aacatcagcg atccgacgct agccgccacg ttgaaaaaca ccatcaacac cgccgttcag
     6781 cagcgtacta ccgtagggct tgtcggcctg gcggtggcgc tttattccgg catcaactgg
     6841 atgggtaacc tgcgtgaagc gattcgcgcc cagtcacgcg atgtctggga acgctcgccg
     6901 caagatcagg agaagttctg ggtaaaatat ctgcgtgatt ttatttcgct gattggtttg
     6961 ttgattgcgc tgattgtgac gctttcgatc acctcggttg ccggttcggc gcagcaaatg
     7021 attattagcg ccctgcacct gaacagcatt gagtggctga aaccgacgtg gcggttgatt
     7081 ggcctggcga tttccatctt cgccaactat ctgcttttct tttggatctt ctggcgcttg
     7141 ccgcgccacc gtccgcgcaa aaaagccctg attcgcggaa catttctggc tgcgattggt
     7201 tttgaagtga ttaaaatcgt gatgacctac accctgccat cgctgatgaa atccccctct
     7261 ggcgcagcat ttggttccgt gctgggactg atggcatttt tctacttctt cgcccgtttg
     7321 acgctgtttt gcgcggcgtg gattgccacc gccgaatata aagacgaccc gcgaatgccg
     7381 gggaaaacgc agccttaaaa taggccggat gcggcgtacg aatttattcg gcctggctcc
     7441 ccgtaggccg gataagatgc gccagcatcg catccggcta taatgcgcac ataacctctt
     7501 gaaactcttt cccagagcct ctttagccat ctattcagga gcaaacaatt tcataccaac
     7561 tcataacccc agcatataaa tccagttggt aacttttatt taacctgaaa ccagttttat
     7621 ccactattta taaaattatg tgaagcattt catagaagaa aaatcactgg cctaaacatt
     7681 atcccctttt tgcctggttt ttgaccattt ccgcgatttg ttacacattg aaatatcact
     7741 tttgctgtgc gtaatatggc tattcgttag ccaaaaaata agaaaagatt atgcaagcaa
     7801 cagccacaac actcgaccac gagcaagaat acacgccgat caactcgcgt aataaagtcc
     7861 ttgtcgcctc tctcattggc acggccattg agttcttcga cttttacatt tacgccactg
     7921 cggccgttat tgtgtttccg catatcttct tcccgcaggg cgatcctacg gcagcaacgc
     7981 tacagtcgct cgccaccttt gccatcgcct tcgtcgcgcg ccccattggc tctgccgttt
     8041 ttggtcattt tggcgatcgc gttgggcgta aagcgacgct ggtcgcctcg ttgctaacga
     8101 tggggatttc gaccgtggtg attggcctgc tgccaggcta tgccacgatt ggtattttcg
     8161 ccccgctgct gctggcgctg gctcgatttg gtcagggtct gggcttaggt ggtgaatggg
     8221 gcggcgcggc tctgctggcg actgaaaacg ccccaccgcg caaacgtgca ttgtacggct
     8281 ccttcccgca gctgggcgca ccgattggct tcttctttgc caacggcact ttcttgctgc
     8341 tttcctggct actgaccgac gagcagttta tgagctgggg ctggcgtgtg ccatttatct
     8401 tctcggcggt gctggtcatt atcggcctgt atgttcgcgt gtcgctgcat gagtcgccgg
     8461 tgtttgagaa agtcgctaaa gcgaaaaaac aggtgaagat cccgctgggt acgctgctga
     8521 ccaaacatgt tcgcgtaacc gtactgggta cgttcattat gctggcaacc tatacgctgt
     8581 tttacatcat gacggtctac tctatgacct ttagtaccgc cgccgcgccc gttgggcttg
     8641 gcctgccgcg taacgaagtg ttgtggatgc tgatgatggc agttattggt tttggcgtga
     8701 tggtgccagt cgccggatta ctggctgatg cctttggtcg ccgtaaaagc atggtaatca
     8761 tcaccacgct gatcatcctg ttcgcgctgt tcgcctttaa cccgttgctc ggttctggca
     8821 acccgattct ggtttttgcc ttcctgctgc tggggttaag tctgatgggg ctgaccttcg
     8881 gtccgatggg cgcgctgtta ccagagctgt tcccgacaga agtgcgttac accggagcat
     8941 cgttctctta caacgtagcg tcgattctcg gggcttccgt tgcgccgtat atcgcagcct
     9001 ggttgcagac taactacggg ttaggtgcgg tggggttata tctggcggcg atggctggct
     9061 tgacgttaat cgccctgctg ctgacccatg agacgcgaca tcagtcgttg taataagagt
     9121 gaaaagcctg atgcgctacg cttatcaggc cagaatttcc atacaatata ttgaattagc
     9181 aaggatttgt aagccggagg cgttcacgcc gcatccggca tgaacaaagc acactttgcc
     9241 aacaatctaa atctgtttaa ttgccggatg tcagacatcc ggcaattatc atcacttctt
     9301 catctgcgac aaaattgtcc tgcactgatt agcctcccct tcggaaggtg agatcagcgc
     9361 cagtaacgcc gccgccggtg ttaccagcgt tgccagtgcc gccgcaacgg ctccgcgagc
     9421 aatcaacggc ccggctttca ccccagcctg cggattttta aacgtcccac gtacatacag
     9481 cggcgaacgc aatgtgataa tccgaatccc tttactctcc ggatcaatag tcaaatccag
     9541 ctgttccgaa gcaaaacttg ccgtaccggt aacgttaatc aacgcgttct cagtatcgaa
     9601 agcaaaaatc tgcgggcgcg ccacgccgtt ggcaatatcc agattcgccg ccgcacagtt
     9661 cacccgcacc tcatcatcac caaatatcgc accgacaatg tagttaccga cattcagccc
     9721 gacaatctcc atcaggttgc ggctcaccag cccgtcattc atcagcagtt tcaggttgcc
     9781 gttactgttg cctaaaagcg ccgccaccga gttaccgcta ccgcgtagtt ccgcgtcacc
     9841 gttcatttcc cccagcgtct tctgcatcag ttctacatcg ggcatcagtt ctttcagttt
     9901 cagtcgacga gcctgaatat ctgcccgccc ttgcatcggt tttttatcac cttccagatg
     9961 aatattcgcc gcaatgctgc cgcctgccat gccaaatttc agcggttgca ggcgcaggtc
    10021 agcatttttg aggatgatat gagtagaaag atcgctaatc ggcaggctac tgccatgctc
    10081 aatgcgccgc cctttgaagc gaacatcggc atccatgacg tcccatttat cggtttcgaa
    10141 gcggtcataa ggcagcactt tgcccgcagg ctgaacgctt ttttcaccct tcttctgttc
    10201 agaccgtttc gacttttcag ctcctttccc ggaatcaacg ccaattaacg gccctaaatc
    10261 tgccagccgc aattgccgcg actcaacatc accttccagt tttggtcgtg gcttgccggt
    10321 ggtgtagacc agagaaccgt ggatatcgct atcgccaatt cgcccattaa aaccgcgata
    10381 atcaaagacc gacgattttt cagtgtcgat tttcgctacc agccgaccat ccgtttcaaa
    10441 cggcggggta tcgggcagca gaacgcccgt cagttcatag agatcaccca gcgaatcgcc
    10501 agaaaattta agccgtaaat cgacaccacc catcttcatt gggtcattca caacgccatc
    10561 aaaagcaaca cgggtgttac cagagcgaaa atcagcctgt acaggaaatg gcgtcccttc
    10621 gccacgcagc gccagcatac cgcctatttt tcccgtacca gtgagcggct ctccgttata
    10681 acgtccctgc gccttcaggc caaaaacgta atcgcccacc ttttctttat ccgctttacc
    10741 tttcgatcca gtaacttcgc tgaacggcag cggcttgcct aacggatcga caaaaatttc
    10801 tagatccgct ttgcttactt tgtcatcaat ggcgatccgc ccttgatcga acagaatatt
    10861 atccagccga aacgaccatg ccgacggctt tgcattcgtg tctttgttgt catcgctggc
    10921 gagattaaat gtccagttgt tgttcttttc tgagaggcga atcaggcgtg cgtcgggctt
    10981 ttcgagcttg atccacggca accagaccgt tttggtcagc agcgccagcg gggccagcgt
    11041 cgcttctact cgcggcaaat gcaccattgt gacttcggga atatccggtg gattgccaag
    11101 aatgatgtct tccgcgtgta catggggcca cggcacccag ctgcgccagc cagtttcttg
    11161 tttttgccgc tcccacacca cgcccagatc gccacggata gcgaacggac gattcaactc
    11221 cgcagagact ttctggttga tggtcggttt gagtcgattc cagtcaaaag ttgcaatcaa
    11281 aatgatcgcc acgacaatca acaacaagaa agcccctgaa atcgcagcgg ttattttgcc
    11341 tgccttgctc atcgttcgct tcctcatatt cttcctgtgc cagtcctaaa gatagtccag
    11401 ccaggcggaa aatgaggcag cttatagcgc cagaaccgcc gtattcagcg tttctatcgg
    11461 tgccgggcgt gaaagaaacc agccttgtgc ggcgaatgcg ggcgagttct gaacatcacg
    11521 ccactcttcc ggcgtttcta cgccctcgac aatcaccccg cgacaatagc gattcatgag
    11581 atgtaaaagc tgagaaaaga gtgtgcggcc ttccggcgac tgacgcagca tcacgaacag
    11641 ctctcgcgcg attttgatgt agtcataacg cacttcactt agcgcagaga aatttgccat
    11701 cccggtgcca aaatcatcca gccacagcgg gccaaattca cacatcgagg caaaggttga
    11761 atctttcggc agacggatat gctccaccag ttcgaaacgc agccagggaa gacgctcaat
    11821 ttggcgcagg atttttggtt gctgacgcag ggcgatgagc gtagggccat caatattgac
    11881 cgatgccagc aggtcgtgct ctataaagaa gtcggctttt tgcgccagca aatcaatctg
    11941 ctctttcaca acctccatcc gatggctaac ggtgatttca gtaaaatagc gatccggcgg
    12001 caggcgttgc gaagggttca agggatgcgt gaccaccgtt aatagctcca cggccattaa
    12061 ccgtccgcat gtttgataga tcggctgcca ggtgtaagca cgctcacact gcaaccaaaa
    12121 acgccgttcc tgcaagctct cgatgcttgc ttcagggttg cttattcgct ggataacctg
    12181 ccttatcatc aaagatgtcc tgtgtttaaa agtgatgctg cccggactcg acaaagatta
    12241 tcggcgcgca agggcagaac tttaccgcct ggtcggggta aatttcccca ctccacaacg
    12301 gaatctttca ggaatacgtt ctggctcaaa aaaataatgg aacactgttt taatatggtt
    12361 gaccagcaaa ccaccacagc gcaaactaac gctaattttt tacagatcag gttcacgact
    12421 atgtccaaaa agattgccgt gattggcgaa tgcatgattg agctttccga gaaaggcgcg
    12481 gacgttaagc gcggtttcgg cggcgatacc ctgaacactt ccgtctatat cgcccgtcag
    12541 gtcgatcctg cggcattaac cgttcattac gtaacggcgc tgggaacgga cagttttagc
    12601 cagcagatgc tggacgcctg gcacggcgag aacgttgata cttccctgac ccaacggatg
    12661 gaaaaccgtc tgccgggcct ttactacatt gaaaccgaca gcaccggcga acgtacgttc
    12721 tactactggc gtaacgaagc cgccgccaaa ttctggctgg agagtgagca atccgcggcg
    12781 atttgcgaag agctggcgaa tttcgattat ctctacctga gcgggattag cctggcgatc
    12841 ttaagccaga ccagccgcga aaagctgctt tccctgctgc gcgaatgccg cgccaacggc
    12901 ggaaaagtga ttttcgacaa taactatcgt ccgcgcctgt gggccagcaa agaagagaca
    12961 cagcaggtgt accaacaaat gctggaatgc acggatatcg ccttcctgac gctggacgat
    13021 gaagacgcgc tgtggggtca acagccggtg gaagacgtca ttgcgcgcac ccataacgcg
    13081 ggcgtgaaag aagtggtggt gaaacgcggg gcggattctt gcctggtgtc cattgctggc
    13141 gaagggttag tggatgttcc ggcggtaaaa ctgccgaaag aaaaagtgat cgataccacc
    13201 gcagctggcg actctttcag tgccggttat ctggcggtac gtttgacggg cggcagtgcg
    13261 gaagacgcag cgaaacgcgg gcacctgacc gcaagtaccg ttattcagta tcgcggcgcg
    13321 attatcccgc gtgaagcgat gccagcgtaa aagcagcata tgcaaaaggc tggatagagt
    13381 gttacccccc tatccagctc atccataaat acctgatggt tgtgctaact catcaccatc
    13441 aggcattact gtacaggtgg aatatccgtc acttcaggat gtacgtcatc cgtggcgaca
    13501 gatgtggcgg cagccgcagt cgatggggcc atgatttgat cccaggccgc ctgcaacgct
    13561 ttcatgttaa attccggctc gcctttcggc tgcagcagta ttaacgccat atcattcgac
    13621 aactgctgac gcagatcctg atttaacatc tccaccgtca ggctgttaag gaaatcctga
    13681 cgcagtttct gatattgctc cggcgcgata tcgacgacct gattttgcaa cgaacgcatc
    13741 cgctgcccca tcagaatatc ggtatcagct cgcgcatagg cggcaaacag tttctgcagc
    13801 tccagtttct tttgcgccac taacgcattg aactcttctt ccggcagacc tttatcgcga
    13861 accttcgcca gttcacgcgc caccagatta aggttgctgt tcagcttgtc gtttggtgat
    13921 tcgatgttga tggcacactg cgcacgcaga tacagcacac gacagtcaaa tccaagaccg
    13981 atgtctttac tgttactggc gcttaacgct tgctgaacat gccagaacag cgcctcacgg
    14041 gccaggtccg cacgccagta gcgcagcagt gcggctgatt cacgaatcgg ctgccacggc
    14101 gtatcccaca tgattgataa ccggtcctga cgcaccgcgt cagtcataat actcaccgct
    14161 tccgcacgca gcggagaaag cgtcggcacc ggagccggtg tttcacgttt gcctttcagt
    14221 tcgccaaacg ttttattgat ttggtcgacg accgagcgcg catccacgtt tcccaccacc
    14281 agcagcgtca ttgcatccgg ggtataccat ttctgataga aatctttaat cttttccgct
    14341 tcaacgggtt gtttcagcgg atcggcagga tcgtgaccta acaaggttga tcctttcaga
    14401 cgatagcgcc accagccctc tttagtatcg gcaggccagg ttgccaccat gtcctgactt
    14461 tgcagcgcgt ggttgatggt ttctggtgtg atggtcagtt tgccagtggc atttgccaga
    14521 taagagagcg cttctttcag caagtcgtta cggttattgg gcaaactcag attaaacagc
    14581 gtggtgtcat aagagacaat tactggcggc atcgggcgtt tagggtcgat cccctgctgc
    14641 cacaatgaac gcgcctgtgc tgcttcaagg ccaccgcttt gcgttaacgc aatacgaggg
    14701 atggcgtggc tgtaaccgct ctgttgtgta ctttcggcga gcgaaccggt attaaccagc
    14761 aggcgaattt caacacgatc gctaggacgc tggggcgtgg tcagcacttg ccactgtaaa
    14821 ccgttggaaa gcgtcccctg ttgccatgct ggatcaggct ggagcgcatc tgcctgcaca
    14881 tagccagcag tggccatcat cagcaaaccg cccgctaaaa gtcgaatttt tgtgccctgc
    14941 atgtgaaccc ctgatcaact atcctggtaa taaaaaaact gcccgttgtc ggtcagtcta
    15001 aatgacgtta aaaacacttc gtgttagacc gcaagaacat gaaaatgtca cggaagaagt
    15061 gaaataaacc tgaactcacc caggtggtaa gttcaggcac aggggtcaat tatgcgcaaa
    15121 cacccgcact cggggaaggg agtgcgggca taagtgatga gattaagagg ataattcgtg
    15181 cgttttgcca tccggcgcac gattattcag cacatcgtcc agttttttgt ggtccagttc
    15241 tttcacccac ttagcaacga caatggtcgc tacgccgtta ccgaccaggt tagtcagcgc
    15301 acgagcttct gacataaagc ggtcgatacc aaggatcagc gccagacctg ctaccggcaa
    15361 atggcccacc gcagagagcg tcgccgccag cacgataaag ccactgcccg ttaccccggc
    15421 agcccctttg gaagagagca acaacacaat taatagcgta atttggtgga cgatatccat
    15481 ctgactgtta gtcgcctggg cgataaacac cgccgccatt gtcaggtata tcgatgtgcc
    15541 atcaaggtta aacgagtagc ctgtcgggat gaccagcccc accaccgatt tccggcagcc
    15601 gagtttctcc atcttgtcga gcatacgcgg cagcgccgac tcggaagaag aagtgcccag
    15661 tacaatcagc agctcttcac ggatgtagcg gataaatttg aagatactga aaccagtcgc
    15721 tttagcgatt gaacccaata ccagcaccac aaacaggata caggtgatgt agaaacagat
    15781 aatcagctgc cccagttgca ccagtgtgcc gacgccgtat ttaccgatgg taaacgccat
    15841 tgccccgaac gcaccaatag gtgccagacg catgatcata ttgatgatgc cgaagatgac
    15901 ctgcgagaaa ctttcgatga cgttaaaaat cagttggcct ttgctgccca gacggtggag
    15961 cgcaaaacca aacagtacgg caaacagcag cacctgcaga atgttaccgc tggcaaatgc
    16021 gccaatgacg ctcgccggga tgacatccat aatgaaggcg acaatgccct ggtctttcgc
    16081 ctgatcggcg taaaccgcta ccgctttcgc atcaagcgtt gccggatcga cgttcattcc
    16141 ggcaccaggc tgcacgacgt taacgatgat aagaccaata atcagcgcga tggtactgac
    16201 aatttcaaag taaagcagtg cgactgcgcc ggtacgacca accgccttca tgctttccat
    16261 gcccgcaatg cccgttacga cggtacaaaa gatgacagga gcgatgatca tcttaatgag
    16321 cttaacgaac ccgtcgccaa gcggtttcat ttgctcgcct atttcagggt agaaatggcc
    16381 aaggagaata ccaatggcta tcgctgtcag gacctgaaag taaaggcttt taaacagaga
    16441 ggttttcata gggtgtcctt tagtaaaacc acaggtcttg taaggttatg gggtacctgc
    16501 ggccctaaaa taacacccag acaacatcac agaaatgtac ctggatcata attgaaacaa
    16561 aaaggttaaa aagtttgagc tggctcgcac aaaccagcac tttttaaagt tttgtaatca
    16621 gtttggggta gctacttttc ttccaggtaa ctctcttcga agatttcaat agggagtggg
    16681 cgagcaaaaa ggaagccctg ggcaatacca acgcccgctt ttgccagcca gtcgcgttgt
    16741 gcttcagtct ccacgccttc ggcaatcatt tgtaagttca ggctttgcgc caacatgata
    16801 attgcagcaa tcatgctgct atcttccggc aagccttcaa caaacatttt gtcgattttc
    16861 agtacgtcga ttggcaacga tttcatatgc tgcagctgac gcagccctgc gtagcccatg
    16921 ccgaaatcat ccagcgccac ccgaactccg gcattgcgca gcggacggag gattgccacc
    16981 gcagcatgag ggtcgtcaat acgtcggctt tctgtcactt ccagaatcag tgttcccggc
    17041 tgaatgcgat agcgggttaa cagttccagc atatccgcta ccatattcgg gtgcatcagt
    17101 tgtagcgctg agaggtttac cgacaagggc agcataatgc cgcgctcttg ccaggcagca
    17161 agcagtcgac aagactcttc cagcacccag tgaccaacgg taaccatcag cccacagcac
    17221 tcaatgcgat cgattaagcc atccggcaga tcccaactgc catccggttg ctggatacgc
    17281 agtaacactt ccgcactgac cagtttaccg ctggtcatct cgacctgtgg ctgtaaccaa
    17341 atcgcaaatt gatgattttc cagtgcatta aggatatcgc tctcttccgt caaccgctgc
    17401 tgggcggctt ccatctgctg cggatcaaag aactgaatct gatttttgcc tttatggcga
    17461 gcggtaaatg ccgcagaaat agcgcgactg taaagctgtt cggcggtgag atcgccgtag
    17521 aacatcgcca cgccaatgct acagtgcgga cggagttgaa tacgttcaat cggcaggcgc
    17581 tcgctcatga tagtgagcac ttgctgacct aatgtgatag cgtgccacgg ttcctgtaca
    17641 ccgttggcaa tgacagcaaa gtcataaccg ctaatctgcg cgaggatcat gcgtggcgac
    17701 agtaccgatt tgagtttttc caccagcgtc agcagcagaa tttctcgttg cgcctctttc
    17761 agcacgcccg cagtatcacg cagggtttca caggtgatga tcatcagcgc ggtggtttgt
    17821 ttacgcgcga caacctgctc cagcatctcc atcagcaagg ctttgttcgg caaatccgac
    17881 accgggaagc gcatcgcgtt ttcgttctgt tcttcataat ggcgctgcag caattgctgg
    17941 ttgaggttgt aactgcgcac cagcataccg atttcatcgt cctgatgcag acgtggtaat
    18001 gccagttggt gaccaacaag ctcctgggct gggatggcgt taagttcgcg agcaatattg
    18061 cgtaacggat gtaaaatcag tcgattaata caccagctga tggcgacggt caggataagc
    18121 gacaaaagta agtaaatggt cactaacgtt gagagggtgc tcatcacgaa cttatacata
    18181 cggaaggaat ccgcctgtag caccagatag gcaattggct gcggatttgc cggacgttcg
    18241 agcgagtaga cgcccagcga gatttgaacc ggtagctcaa acaggcgagt aaccattacc
    18301 ggcaccggac gctctggaat aaaactttta cgcagcgcct ggaactggtt gggcagcacc
    18361 acatcggcac ggctgaccac gcctgccggt ttaatgctgg caagaatagc ttccgcttcg
    18421 ggaatatcgc ctttcaaaat agctgaagat aagggttcgc ggacagagcg agcaatgctt
    18481 tccagttgcg tagccgtgtt atagcgattc tgctggacca gatggaacag caaaacggtg
    18541 caaaaaataa aaacgaacac caggacaacg gctgccacca ttgccatctg cttgattgtt
    18601 aacgagcgac ttacgcgcaa attgtctccc cccggaaatc ccagattgcc gcccgagtat
    18661 acccgatcgc agcggcatta agagaggcgc tatctgaaaa cttaccagtc ggcgtaaggt
    18721 atcagcggct gcggcggtaa atccatgtca ccctgccatc cggcggcgga ataacgtacg
    18781 tagagcagga aatggctggg tgcgtaatct ttcgcctgct ggatatcaat tgccgtgccg
    18841 acaaaccagt tggaagtaac acgtcgttca agtaatgccc gcgccgtata gccgaagccc
    18901 tgactgctgc cgccatcgtt ggattgccgc gcagcttctt cctgccagtc ggtcgggatc
    18961 agattcatca gcggataacg cggcatggtt ttggtgcgtg aatgcgacca cgagccagac
    19021 gcccccagct cccacgacca gttttccgtg cgctcccgcc acatcaccgg tatggcaaac
    19081 gacaggtatt cctgcgggct gtagtaaccg ccctgaccga gtgagtagcc actcaggtct
    19141 ttgtcgtaat gccagatcat gttgttcagg ccgattgtga cgcggcgatt gttctggtta
    19201 atgaccttat agtaatagcc cgtcatccag cgcacacgcc agttatcttc gacattttta
    19261 ccggttaact ggtcgccact aagcgatgcc cagacgccgt ttgcttcacc tttatcgtag
    19321 ctcaggctta gccccacgcc gtcggcacgc acgccgcccc attttttccc ggtattgctc
    19381 ggggagtctt tttgcccacc aaaggccagc aaagaactgg agatgggccg acggtgggcg
    19441 ttaacggtgt aacccagcgg cccgatatca tcgctgtaac tgatgccgcc gaccacatcc
    19501 accacgttga agcccatcgg cgtggtgccg atatcccagc tccaggtgtc gttgcgccaa
    19561 ccgaccgcca cgctggcacc ggaatccgac tggctgcggt tgccgctaca gtcctgtaat
    19621 gtacaggtgc cccagttgtc atcccattta ccatcggcat tagtggagaa actgccgacg
    19681 ttcatattga cgaaatcact gcgaaagaac atccgcccgt cagaatacgg cgcatccacc
    19741 tgcaacatgg tagtgtgcgc tttcagatcg gagtaaccac cggtgccgct cgaaccccag
    19801 taatcatgct caagggtgac gttaagatcc tgctggcgat agaggtccgc cgcatcgctg
    19861 cgcacgccgc gtttcagcca gtcatctttc tcgtcattgc gggtcagtcg ggtaaaggtg
    19921 tcgttatcca gcggacgcgt cgtggtcaca ccggatgcaa ccatcgcgtc tttgtaggtt
    19981 tccagcgcct gcgttggatc gcccgcctgc gcttcaaact tcgcaccatc acgcagtacc
    20041 atcgcacttt ccatcgacgg cggctgagat tttgccagcg ggatcaactt attaaacgtc
    20101 tgctgcgccg ctgcggtatc gccaagctgc gcctgtgcca gcgccacgcg ccgctgagtg
    20161 ttcagcgagg cgttatcggt ggcaggcagc ttcgccagct ggctacgtgc cgccgctttg
    20221 tcaccggcag caatatccac ttccgtcaga ccaagaatgg catcggcgtt agttggctcc
    20281 cgcgtcagga cattctgata tgcagcgcga gcggcggtgt aatcacgtcg ttgttgcgcc
    20341 cagtcagcca gcgtgaggtc tatgcgcgtg gaaggtggtt gctggcgcag catcgcttcc
    20401 gcttcagctt ctttgccgct ttctcgcagg cgattagcgg tttccaacac ctgatcgctt
    20461 tgcagtcgat taaccagctc ctgaatattg ctgctccact gcgcgcgcgg caggctgttg
    20521 atatgcgcca gcgccgctct gtcctggtca tgaccagaga gatacagccc gtaagcgtaa
    20581 acctgctccg ggtcgttcgg cttctgctgc gccagattac gcattaacgt atcggcctgg
    20641 ctgcgttgtc cggcctggct gcgttgtccg gcctgccaga gatcctgtga aagtcggtaa
    20701 gtaatccata cactgccggg gtccagcgcc agtcgttgcc gctgaagtgc tgccgcctgc
    20761 gcccatttcc cctggttttc cagtgcctct gcctgctgtg ccagacggtc gttttgcagg
    20821 ctgcgttcga tatcatcaat gctgcgccgc tggctggcag agagcgaggc gatatacgct
    20881 tcggcttttt ctggcgactg ctggcggtaa atattcgcca gcccgcgcac ggcgttagtg
    20941 ttgccgctgt ccatacgcaa ggtctgctgg taatagcgtt cggcggcggg ataatctttg
    21001 cgcgccatcg ccacatcgcc cagcccaagc actgcatagc tgtcggtgtt atcgacatta
    21061 cgtgcctgct ggaacagccg ttctgcccgg tcaggattat tggctttcag cgcggcatcg
    21121 ccctgctgga tcgccagcca gtagcggttt actttcagca gactgttcca tttgtcgtta
    21181 ttgctgttgt gcggatccag cgcgagggct ttttccagat tcgccaccgc attagcgcga
    21241 tcgcctttct gagaatacgc ctggcccagc gcccccagag cctcactgtc tttcgggttc
    21301 gcccgcaccg cctgttgtag ttcgggaatg gctttacccg ccatgccaga gtccaccgcc
    21361 gctaaacctt gcgcacgagc gcggaaagca gggtcggcca gctgtttttg ctgttctgcc
    21421 agttgcgatt gtgcagcagc tacgctatcg ccatcgctaa agatcgagag atattttttc
    21481 agtgcctgaa cgctggcatc gctgacaggc atgtctttaa tctgcccgta ccagatttta
    21541 gaggcccctt cgcgcccggc gttcgatttc gccatctgtt ccaggacggc aaaaccttca
    21601 tcacggcgat cgctactaaa cagtaatagc gccagattgt tttgcaggcc cgtgttgccc
    21661 ggcgtatccg cattaatgcg ttttagctga ttaatcgctt cgccacgtcg ggccggaatt
    21721 ttcgccaccg tactccagta ctcgacagca atgtcacctt ccggcggcgc accgttgaac
    21781 agtttgttgt aactcgccac agcttcttcc gcatgaccag tcgtcgcctg caatcttgcc
    21841 tgttgcagtg cctgacgacc atccgacgtg gaaagcagca tcgtagtccg tgacgattta
    21901 tacgcatttg aactcggcgc taactgcgac agccgatcga gctgtttttg tgcgccatca
    21961 atatcgccct gacgtaacag agaacgaaaa cgggcagcaa cgacgtccgg gttattcgga
    22021 tcaataagtt ccagacgata caacgactgt tgcaccaggt cttcacgatg ggtcgcttcg
    22081 cctaaccgaa cttgctccag caactgttgc tgagcggttg gtgctgcctc gaccatcggc
    22141 atgacggcca gaccgaggga aagcgtgaat atgtttagtg tgaatttgcg cattcctggc
    22201 cccagtcagg taataactca ccttttgtcg agaagcggaa acggtgttga tcccagcctt
    22261 gtccaaacag ggtcagcaca tagttgtaat aggcatcgct gccaggaaag ttatcggcca
    22321 cgcgctggcg ctgaacggcc tgcgcatcgc ggttttgtaa aaagggcagc atggcggcag
    22381 aaaaaccgac cggtccttta ccctgcgctt tccccgtagc cacatccact ttttccggcg
    22441 gataaccgtt tttctcagtg aatgtcgcca tcggtttaaa ccggttgagc atccgcgctt
    22501 tttgcggatc gctgtcaggc atcatgccta cccacatgta aacgcggata gcgtcgtagc
    22561 tgctgatcaa tgttttttcg gcttttagct gccagccttt gtctttctca tagcgcaccc
    22621 agtctggcga aaagcctttc ggggcggttt ccagcaataa acgttgattg gtttcgcgca
    22681 gcgtggtcca cggcgcgcca aagcgggtga aatactgcgc cagcgtcggc ggcaggtagc
    22741 tggggttaaa acgccagctg ttatcctctg caaatcccac tttgcccggt aacaacatgg
    22801 agcccagccc aggcaccgtc accacttcct cccgcgcgat acgttttagc aatgcgctgc
    22861 cgatgtcggt ataacgctgc tctttccaca aacgccccgc ctccagcaac gcccaggcca
    22921 tccagacatc accatcggag gccgaattgc tgtccagcac ttcccactta ctgttctctt
    22981 tcttgcccca cagccaggcg ggcaaatgtt cttttaaaga accctgtgcg agattgttct
    23041 gcgtccagtc gagaagatta tcgaaagctg cacggtcgtt agccgccagg gcaaagaaca
    23101 tgccgtaact ttgcccttcg gaggtggtga ttttgcgcgc gtcgctgggg tcgatgacgc
    23161 gcccttcctg actgatgtaa tcctttttaa actgctccca ggcaggccag gtacaggctg
    23221 cctgaacact aaaggcagcc agcagcagca tcgtcacgag tccactacgc aacacattca
    23281 tcttcaatta ctcgttatcc gggttaagac gacgacggct gataatacgc agcagacgcc
    23341 acagtaccca tgccagcagc acgacgctga ttgccgccag caccgccagc agaatcggat
    23401 ggtttgccag cgcataccat aagcgttcga accacggcag atggccaacg taatagacgt
    23461 cgccaacacg caggctattg ataccggact cgcggatcac cgcgaccgaa ccgaacatgg
    23521 tggcgcgttt gccgctatcg ttcaccgcat cgttaagcat ttcataaccg cgtgggctat
    23581 ctgccagcag cgcaatcacg ctacgctggt cgttatacgg cgactggaag ccaatcaccg
    23641 ctgccatcgc acctgaagag gtcagcgttg actgagtttc tgccgcgcga tcgctctcgt
    23701 ccggtacaat gccggggaac ggggtctggc gcatcggcgt tttcacccag ctttcggtcg
    23761 cctgcaccaa caggtcgatc tgcttgtcgt ctttcagttt gtccgggata ccaccgacga
    23821 tcatgatgtc ggcatctttc ccctgaatag tgctgccatc atcggtcacc gtcaggttaa
    23881 tcgccgggaa gcccgtctgc gcaccgataa agccaacggt attcagcaag gtttccatct
    23941 gtgcttcgtt aggcgctttc ggcatcacgg tgatggtttg tgacagatcc gccatccggc
    24001 tgaacgggaa gcccgcgtta gcaaaggcgc gtagatccgg catcgggatg aagtggtaat
    24061 acttcgagaa gtcgatggtg gaatcatcac caatcaccac atgattctgc accggctgga
    24121 aggtaatgca gttatccacc gaaccgcccg gcatcgggtt catatactca aagtcaaagc
    24181 gcagctggtt ggtcgcgccc agtttcagcg ccggaataga gacatccgtt ttgccatcca
    24241 gcaaaccttg taataccgga atacgcagca gcaggcggtt cgcctcctgt ttactgctca
    24301 ggttgaagga ttgcaggaac tggttattca ggctgatatc catccgtgag ctgtctttca
    24361 ctggcggcat ggtgtagcga taattaatat ccatatcaat gccggtactg cgcatcaggt
    24421 agagatccgg tggcaggttt agcgaaacgt taatcgctgc tggctcaaga ccgctggatt
    24481 gtaactgttc ttcataggtt ttcagttcac caaaggtaac cggacgatcg gtacgcaccc
    24541 agttcggcgc atcgtacggc ttacgcggta gcagcggttt cacttcattc actaccacgc
    24601 tttcaccacg gaacagaatg ttaccctgag cgataccttt cgctgcctgc aacaggtctt
    24661 tgtcgtcacg accaaacacc accagcagtt tgacataagg attctgcgga tggttgatca
    24721 tttcaatcac cggggctttt accgccggat gatcgcgcag gaagtccggg cgtttgtcat
    24781 tggtggcaaa aacgatggca ttgcgatccg gcaattggtt atagagcacc gggaagttct
    24841 gcccacgcca gccagaacgc gaaccaaacc acgaggcgac aatggcagat gcttgttgca
    24901 gcccgacatc cggcgcaccc gcaaagacca tcggcaaggt gttagtacgg ttatcacgcg
    24961 ggtcaaagaa tggcaccggg aagtgtgaca ggtcattctt cacattcagg gtctgatagg
    25021 tcagatccag tccactgctc cgcccaacat ccagccaaag cgtggtgctg gccgggtttt
    25081 cgcacacgtc ctgataatga ccgacaaact ccagccgcac acggttgaag tcggtaataa
    25141 acagtgggtt aattggcatt tgtgccagcg tttttttacc caactgttct ttggtcactg
    25201 gcagcacgcc catcagttca tcattgagat aaacctttaa ctgcgactgg acaggcagta
    25261 atgatggcga tggcgtgtat tcgaggttga gcatcgcctt cgtcaccact tcatcgctgc
    25321 gcataccaaa ctcaatgctg ccgttcgggt taatgccacg tagcaccatg ctgcccggcg
    25381 gcggcgcaat ttgcgcaaag gtcagcttca catcacggga aggaccgttc tgcgccacga
    25441 ctggcgcatc tgcgccctgc acgcccggca tcacttgccc cacctgcgga ttttgctccg
    25501 cctgggcggc tacagctggc tcagcattga tcagtggttg cgttgctggc gtcgcctgcg
    25561 tcatgaaaga ggggaacgca ctcatcccca gagccactgc acaaatccag aatatttttc
    25621 ttttcatcgc gttatcatca ttgttgagcc aaagcctgat ccgatggttg tgccgtttcg
    25681 ctccgctccg ggcggcgcgg aataaacgat acaacccagg aaaccagaga agtcagcaca
    25741 cggaatatgc ccttcaccga agaaggcgca aattccgcca gatggcggta gccacggaag
    25801 ccgagcttca gaatatccag cagactttcc agcggcttat cttccgggta gctgtcctgc
    25861 cagagcgccc atgtatccgc acgggcaaac gtacactgca caaaatcgat atgttgctgg
    25921 gtagtgagcg gcattaattt cagcccaact tcattaccca tcacacgcgc cacctgagcc
    25981 gggaagacgt attcctgctg accgcgttta agcaacagat tcactttctg cccttccaga
    26041 atctgcgcct gaccgttgat cttgatcccc aaaccaccgt cggagaaatc ctgaacggta
    26101 cacgagaaga ggtgaccatc ttcgcgggca attgctgcgg gcatcgtcat ctccacgcgg
    26161 tgcgatcggc gtacctgttt gctttctacc gatactgcga ctgcgccgcc gagaataatc
    26221 aggttgtaga atacccacac catactgacg accacggtga gcatctcggt tggcggacca
    26281 tagaagtagc gccagatccc aaccgctacg cccaccaggt tgagcagaac aaggaagatg
    26341 tagggccgcg agatcaccca gtcgacgtac tcttcttcca ccagtccacc tttggcggtg
    26401 acgttaaatt tgcctttgtg cgggttaatc agcgccacca gcgtcggtgg tgcgatatac
    26461 cacgccagca ctgtttcgta gatttcactc cagaaagagt ggcgatattt gccctggatc
    26521 ttcgagttag tcaggctggc atggatcata tgcggtagca cgaacagggc gatcatcaac
    26581 gctggcgcgt agatgatgta ggcatgaaac agcaggaacg ccagcggcgc agtcaggaag
    26641 atcagccgtg gaatgcccga caagaaatgg aacatggcgt tgacatagca cagccgctga
    26701 gcaaacttca gccctttacc ggtgagtggg ttatcgagac ggaagatttg caccatcccg
    26761 cgcgcccagc gaatacgctg accgatatgc gccgagagac tttcggtcgc cagccccgct
    26821 gcctgcggaa tacgcatata cgctgaggtg tagccacgac ggtgcagacg cagagaagtg
    26881 tgcgcatctt cggttaccgt ttcgacagca ataccgccaa tctcatccag cggcttacgg
    26941 cgaatcaccg cacatgaacc gcagaagaaa gtggcatccc acatatcatt gccgtcctgc
    27001 accagaccat agaacagcgt gccttcgttc ggcgttttac gaaaacgccc caggttgcgt
    27061 tcaaacggat ccggcgagaa gaagtggtgc ggcgtctgca tcatcgccag ctgtttctct
    27121 ttcaggaacc agcccatggt catttgcagg aacgatcgcg ttggtacgtg gtcgcagtcg
    27181 aatatcgaca cgaactcgcc tttggcatat ttcagcgcat tgttgatgtt gcccgctttc
    27241 gcatgttcat gagtggtacg ggcgatatat ttcaccccca cgttttgcgc aaactggcga
    27301 aactcttccc tgccgccgtc atcgaggatc cagatgttca gcttgtcttt cggccagtcg
    27361 atacccagcg aagcgtaaat ggtatttttc accacgttga gatcttcgtt gtaagtcggg
    27421 acaaagatat ccaccgacgg ccacagcgac atatctttcg gcaatggcac cggctgacga
    27481 ttcagcggcc atacgacctg gaagtagccg agcaccagca ctatccacgc gtacgtttcg
    27541 gcgaacagca gaataagccc gcacaccagg ctgaccggat cgtcccagtt taacgtcgag
    27601 gtgtaacgcc accagatata acggcaagaa acggtcagcg acagcacaat cagcatcagc
    27661 gccgagaagc gccccggcat ccgccgtacg atcagcgcta ccccccacag cagcatcagg
    27721 aagataaact gcgctagcgg gttaaacggc tgagtaacgc agattaacgc cagaatcagc
    27781 gagaaggtga cgatgatacc aaggatcaac cgccgcgccc cggcactcaa atgaccgagc
    27841 tcttttttct catcaagatg ctgtgtttta tggctaacgc gctcaggcag ctcgttcatc
    27901 cattgatggt aacgtccacg aatattttgt agacctgaaa atgcccgcct gcgcggtttc
    27961 ggcgtttctt tgcgcgatgc accgatcagt aaccagcatg tttgaataag ataacggacc
    28021 gggtccagcg gacgcggacg cgaggcgttg atatgcggat acaggttttt atgttctgcg
    28081 cgaatacgct gccagcgcgg gtgctccagc ggaataaaaa tccaggccag gatcatccag
    28141 aaacagccga gcgtcgcgct gaaagccgac gcaccgtgac gacgataatc gcgataacgc
    28201 ccaataagcc gtgcgttgac cggcgggata agcaaccacc gggtcaggat actcatgatg
    28261 cactcccgac tggcgttttc agcccggaat aattcaacag gcaccagttc gccagcgtca
    28321 gtatctcttc agccgccagc gcatcactgc gatattcacc gactggttgc ttagctgcca
    28381 gacattcagc catcgcttca tcacgatgaa tgagcatcgg cagcaatcgg cgctggcttt
    28441 gcaaccaaag ctggtaaata tcgtcctgaa cctggctgcc aatacggaag tcattaatca
    28501 aaatatgtgc accatccggc agcgcttgct gatgcagtcg gatatggcag ttggcatcca
    28561 cattgacgat tgccagcgag tgatcgcaca aactcagcag ctggtgggtt atctgcgagg
    28621 catcacgcgg taagtcaatt aaaatccact ggtaacgccc gctggctttt agttgctgta
    28681 agccggtgca aatatcgctc agccgggttt gccagtgctg tggattttct tgttcttcaa
    28741 tcgataactg accaaaaggc agcaaatcga gctgcgaggt atagcgcaac ccggcgtcac
    28801 gccagtcctg gccatccagc atagctctgg cccaaccctg acggtgggta aaatcaacgt
    28861 taaatgacag gcgcaacaag ttgtccgggc aggcatcgac caccaggaca ttttctccca
    28921 acatttgtaa tgaccaggct aatgcggcgg tgatggtt
//
LOCUS       DAZGQL010000069        28914 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.069, whole genome shotgun sequence.
ACCESSION   DAZGQL010000069 DAZGQL010000000
VERSION     DAZGQL010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28914
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.069"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            171..1850
                     /gene="ycjM"
                     /locus_tag="ACHKIR_003277"
     CDS             171..1850
                     /gene="ycjM"
                     /locus_tag="ACHKIR_003277"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HFO9990964.1"
                     /translation="MEQKITDYLDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPTFNQFYHQWLQSTFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQQPGFEDFFIAVDP
                     QTDLSAVTRPRALPLLTPFQMRDNSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LEKGAEYIRLDAVGFMWKEPGTSCIHLEKTHLIIKLLRSIIDNVAPGTVIITETNVPH
                     IDNIAYFGNGDDEAHMVYQFSLPPLVLHAVQKQNVEALCAWAQNLTLPSSNTTWFNFL
                     ASHDGIGLNPLRGLLPENEILALVEVLQQEGALVNWKNNPDGTRSPYEMNVTYMDALS
                     RRECSDEERCARFILAHAILLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKY
                     SSKEITTELNNKATLRYLIYYKLSHLITLRRSHKEFHPDNNFTIDAINSFVMRIQRSN
                     ADGDCLTGLFNVSENIQHINITDLHGRDLISEVDILGNEITLHPWQVMWIK"
     gene            1864..3156
                     /gene="ycjN"
                     /locus_tag="ACHKIR_003278"
     CDS             1864..3156
                     /gene="ycjN"
                     /locus_tag="ACHKIR_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000597433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO9990965.1"
                     /translation="MIKSKIVLLSALVSCALISGCKEENKNNVSIEFMHSSVEQERQA
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYAKVMDKE
                     QLIDRKAVATVISNVGEGAFYDGVLRIVRTEDGSAWTGVPVSAWIGGIWYRKDVLAKA
                     GLEEPKNWQQLLDVAQKLNDPANKKYGIALPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDTPEMMQALTYYRNLAANTMPGSNDIMEVKDAFMNGTAPMAIYSTYILPAVIKE
                     GDPKNVGFVVPTEKNSAVYGMLTSLTITVGQKAEETEAAEKFVTFMEQADNIADWVMM
                     SPGAALPVNKAVVTTATWKDNDVIKALGELPNQLISELPNIQVFGAVGDKNFTRMGDV
                     TGSGVVSSMVHNVTVGKADLPGTLQTSQKKLDELVEQR"
     gene            3177..4058
                     /gene="ycjO"
                     /locus_tag="ACHKIR_003279"
     CDS             3177..4058
                     /gene="ycjO"
                     /locus_tag="ACHKIR_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFO9990966.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            4045..4887
                     /gene="ycjP"
                     /locus_tag="ACHKIR_003280"
     CDS             4045..4887
                     /gene="ycjP"
                     /locus_tag="ACHKIR_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFO9990967.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSMVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            4918..5970
                     /gene="ycjQ"
                     /locus_tag="ACHKIR_003281"
     CDS             4918..5970
                     /gene="ycjQ"
                     /locus_tag="ACHKIR_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFO9990968.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPQGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCEIARRHGADFCLNPIGSDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            5989..6777
                     /gene="ycjR"
                     /locus_tag="ACHKIR_003282"
     CDS             5989..6777
                     /gene="ycjR"
                     /locus_tag="ACHKIR_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFO9990969.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNLEEV
                     KAAIKETGLPVITACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGGKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLRVLEKVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENDLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALLEQLRADNFQGYVVYEGRIRAEDPAQAYRDSLAWLRTC"
     gene            6787..7842
                     /gene="ycjS"
                     /locus_tag="ACHKIR_003283"
     CDS             6787..7842
                     /gene="ycjS"
                     /locus_tag="ACHKIR_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFO9990970.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALAEKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPPAMTAEQAREMCDTARKQGKVLAYDFHHRFALDTQQLREQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVTAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQREMADDNRHLRSMEAFINHVQGKPVMIADAEQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            7839..10106
                     /gene="ycjT"
                     /locus_tag="ACHKIR_003284"
     CDS             7839..10106
                     /gene="ycjT"
                     /locus_tag="ACHKIR_003284"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HFO9990971.1"
                     /translation="MIRPVTLTEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDVVGMEIAINGEIFSLSREAWQRELNFASGELR
                     RNVVWCTSNGAGYTITSRRFVSADQLPLIALEITITPLDADALVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCKVSGDVQQCYTAKERRLLQHTC
                     AQLHAGETLTLQKRVWIDWRDDRQAALDEWGSASLRQLEMCAQQSYDQLLAASTENWR
                     QWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQATGDESFIAHEGMALLLGTAK
                     FWISRAVQVNDRLEIHNVIGPDEYTEHVNNNAFTSYMAYYNVQQALNIARKFGCSDDA
                     FIHRAEIFLKELRLPEIQPDGVLPQDDSFMAKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQYSAASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LAQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRNGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            10103..10762
                     /gene="pgmB"
                     /locus_tag="ACHKIR_003285"
     CDS             10103..10762
                     /gene="pgmB"
                     /locus_tag="ACHKIR_003285"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFO9990972.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFKAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNPQERAQLAYRKNLLYVHSLRELTVNAVLPGIRNLL
                     ADLRAQQIPIGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWPRLSAFWQN
                     V"
     gene            10776..11858
                     /locus_tag="ACHKIR_003286"
     CDS             10776..11858
                     /locus_tag="ACHKIR_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO9990973.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKERNIAMVFLNYALYPHMTVYDNMAFGLK
                     MQKIANEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSPAMNFIRGTIDGDKFVTETLKLTISEEKLAVLKTQESLH
                     KPIVMGIRPEDIHPDAQEENNISAKISVAELTGAEFMLYTTVGGHELVVRAGALNDYH
                     AGENITIHFDMTKCHFFDAETEIAIR"
     gene            11903..12808
                     /gene="ompG"
                     /locus_tag="ACHKIR_003287"
     CDS             11903..12808
                     /gene="ompG"
                     /locus_tag="ACHKIR_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HFO9990974.1"
                     /translation="MKKLLPCTALVMCAGMACAQAEEKNDWHFNIGAMYEIENVEGYG
                     EDMDGLAEPSVYFNAANGPWRISLAYYQEGPVDYSAGKRGTWFDRPELEVHYQFLESD
                     DFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGWLSMYKFAN
                     DLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFSTQEIRAYL
                     PLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNGLSVSLEYA
                     FEWQDHDEGDSDKFHYAGVGVNYSF"
     gene            complement(12859..13857)
                     /gene="ycjW"
                     /locus_tag="ACHKIR_003288"
     CDS             complement(12859..13857)
                     /gene="ycjW"
                     /locus_tag="ACHKIR_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO9990975.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNLAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGNQNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRD
                     EWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLIAGKPSPQNITVATHMSLK"
     gene            14012..15409
                     /gene="ycjX"
                     /locus_tag="ACHKIR_003289"
     CDS             14012..15409
                     /gene="ycjX"
                     /locus_tag="ACHKIR_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HFO9990976.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRYKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTIDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            15406..16467
                     /gene="ycjF"
                     /locus_tag="ACHKIR_003290"
     CDS             15406..16467
                     /gene="ycjF"
                     /locus_tag="ACHKIR_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjF family protein"
                     /protein_id="HFO9990977.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIEDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            16615..18156
                     /gene="tyrR"
                     /locus_tag="ACHKIR_003291"
     CDS             16615..18156
                     /gene="tyrR"
                     /locus_tag="ACHKIR_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HFO9990978.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(18200..18706)
                     /gene="tpx"
                     /locus_tag="ACHKIR_003292"
     CDS             complement(18200..18706)
                     /gene="tpx"
                     /locus_tag="ACHKIR_003292"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HFO9990979.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            18825..19790
                     /gene="ycjG"
                     /locus_tag="ACHKIR_003293"
     CDS             18825..19790
                     /gene="ycjG"
                     /locus_tag="ACHKIR_003293"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HFO9990980.1"
                     /translation="MRTVKVFEEGWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQESLADLIGITLPGTVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            complement(19765..20493)
                     /gene="mpaA"
                     /locus_tag="ACHKIR_003294"
     CDS             complement(19765..20493)
                     /gene="mpaA"
                     /locus_tag="ACHKIR_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HFO9990981.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASHESGLI
                     LAGTHGDENSSIVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPL"
     gene            complement(20784..21422)
                     /locus_tag="ACHKIR_003295"
     CDS             complement(20784..21422)
                     /locus_tag="ACHKIR_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO9990982.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSKIIMGDVLNHAALKQAMQGQDVVYANLTGEDLDIQANSVIAAMKAGDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPGTDGDKPFFM"
     gene            complement(21493..22413)
                     /gene="ycjY"
                     /locus_tag="ACHKIR_003296"
     CDS             complement(21493..22413)
                     /gene="ycjY"
                     /locus_tag="ACHKIR_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO9990983.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYPAIVVSHPGG
                     GVKEQTAGTYAEKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDVSAVIDYLTT
                     LSYVDNSRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPYVDAGSNARTSDISSREYATMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPIQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKVYVAEAISVLAPFFEETL"
     gene            22551..23450
                     /gene="pgrR"
                     /locus_tag="ACHKIR_003297"
     CDS             22551..23450
                     /gene="pgrR"
                     /locus_tag="ACHKIR_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HFO9990984.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALTSLSDLQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSAPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
     gene            23787..25400
                     /gene="mppA"
                     /locus_tag="ACHKIR_003298"
     CDS             23787..25400
                     /gene="mppA"
                     /locus_tag="ACHKIR_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HFO9990985.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKAAPDQLGVTA
                     VDAQTLKIQLDKPLPWFVNLTANFAFFPVQKANVESSKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPVYDKVLAQASTENTVKARNADYNTAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(25451..26482)
                     /gene="ynaI"
                     /locus_tag="ACHKIR_003299"
     CDS             complement(25451..26482)
                     /gene="ynaI"
                     /locus_tag="ACHKIR_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HFO9990986.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPDQGR"
     gene            26726..26983
                     /gene="ynaJ"
                     /locus_tag="ACHKIR_003300"
     CDS             26726..26983
                     /gene="ynaJ"
                     /locus_tag="ACHKIR_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HFO9990987.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            complement(27033..27983)
                     /gene="uspE"
                     /locus_tag="ACHKIR_003301"
     CDS             complement(27033..27983)
                     /gene="uspE"
                     /locus_tag="ACHKIR_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HFO9990988.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(28135..28887)
                     /gene="fnr"
                     /locus_tag="ACHKIR_003302"
     CDS             complement(28135..28887)
                     /gene="fnr"
                     /locus_tag="ACHKIR_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HFO9990989.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
BASE COUNT         7189 a         6866 c         7580 g         7279 t
ORIGIN      
        1 tgtcacacct tatttacatc cccatagatt acttgcgtca gctcacaaat acgctttttc
       61 cctggtaaaa aatgatttcc tgcgtgacta aaacccttgt gctcaattga cagtttattt
      121 tctgcggagt agtctctcgt ttcatgggac cgctaccacg aaaaggcaac atggaacaga
      181 aaattacgga ttacctggac gaaatctacg gtggaacatt taccgcgact catttacaga
      241 aacttgtaac gcgtcttgag agtgcgaaac gattaattac acagcgacgt aaaaaacact
      301 gggatgaaag tgatgtcgtg ttaattacct atgccgatca atttcacagt aatgatttaa
      361 aaccactacc cacatttaat cagttttacc atcaatggct gcaaagcact ttttcacatg
      421 ttcatttgtt gccgttttat ccatggtcat ctgatgatgg cttttcggta attgattatc
      481 atcaggtcgc cagtgaagcg ggggagtggc aggatattca gcaactcggt gaatgcagtc
      541 atttaatgtt tgattttgtc tgcaaccata tgtcggcaaa aagtgaatgg tttaaaaact
      601 atttacaaca gcagccaggt tttgaagatt tttttattgc ggttgatccg caaaccgatc
      661 tcagcgccgt cacccgtccg cgtgcgttac cgttattaac gccattccag atgcgcgata
      721 attcaacgcg tcatttatgg accaccttta gcgacgatca aattgacctc aattaccgta
      781 gccctgaagt gttactggcg atggtggatg ttttactgtg ttaccttgag aaaggtgctg
      841 agtatatccg tctggatgcc gttggcttta tgtggaaaga gccgggaaca agctgcatcc
      901 atctggaaaa aacacatctg attatcaaac tgttacggtc gattattgat aacgttgcgc
      961 caggcacagt gatcattacc gagaccaatg ttccgcatat agacaacatt gcttactttg
     1021 gcaacggtga tgacgaagcg catatggtgt accagttctc gctgccgccg ctggtgctgc
     1081 atgcggtgca aaaacagaac gttgaggcac tttgtgcgtg ggcgcaaaac ctgacactac
     1141 cttccagcaa caccacctgg tttaacttcc ttgcctctca cgatggcatc gggcttaacc
     1201 cactgcgggg gttgttgccc gaaaacgaaa tattagcgct ggtcgaggtg ttacagcagg
     1261 aaggggcatt agtaaactgg aaaaataatc ccgacggtac gcgtagcccc tatgaaatga
     1321 atgtcaccta tatggatgcg ttgagccgcc gtgagtgtag cgacgaagaa cggtgtgcca
     1381 ggtttattct cgcccatgcg attttgttaa gtttccccgg tgtgccagcg atatatattc
     1441 aaagcattct ggggtcgcgt aatgattacg caggtgtcga aaaacttgga tataatcgtg
     1501 cgattaaccg taaaaaatac tctagtaaag agataacgac agaactgaac aataaagcga
     1561 cattaaggta tttgatatat tataagttgt cgcatttaat tacacttcgt cgcagtcata
     1621 aagagtttca tccggataat aatttcacca ttgatgcgat taattcattt gtaatgcgaa
     1681 ttcaaagaag caatgcggat ggcgattgcc tgacgggatt gtttaatgtc agtgaaaata
     1741 ttcagcatat aaatattact gacctgcacg gtcgggatct gattagtgaa gttgatatat
     1801 tgggtaatga aataacactg catccctggc aggttatgtg gattaaataa aaaggaacat
     1861 ttcatgatta aatcaaaaat cgtgctgtta tcagcactgg tttcctgcgc cctgatttca
     1921 ggctgtaaag aagaaaataa aaataatgta tccatcgaat ttatgcattc ttcggtggag
     1981 caggagcgcc aggccgtcat cagcaaattg attgcccgtt ttgaaaaaga aaacccaggt
     2041 atcaccgtaa aacaagtgcc cgtggaagaa gacgcctata acaccaaagt cattaccctt
     2101 tcacgtagcg gttcgctgcc ggaagtgatc gaaaccagcc atgactacgc caaagtgatg
     2161 gacaaagagc agcttatcga tcgtaaagcg gttgccacgg tcatcagcaa cgtcggtgaa
     2221 ggcgcgttct acgatggcgt actgcggatt gtgcgcaccg aagatggcag cgcctggacc
     2281 ggtgttcctg tcagcgcatg gattggcggg atctggtatc gcaaagatgt gctggcaaaa
     2341 gctgggctgg aggagccaaa aaactggcag caattgctgg acgttgcaca gaaactgaat
     2401 gacccggcga ataaaaaata cggtattgcg ctgcctacag cagaaagcgt gttgacggaa
     2461 cagtctttct cccagtttgc gttatccaat caggctaacg tctttaacgc cgaaggcaaa
     2521 atcacccttg atacaccaga gatgatgcag gcgctgacct attaccgcaa ccttgctgcc
     2581 aacaccatgc caggttctaa cgacatcatg gaagtgaaag acgcttttat gaacggcacc
     2641 gcgccgatgg cgatttactc cacctatatc cttccggctg tgattaaaga aggcgatccg
     2701 aaaaacgttg gtttcgttgt acccaccgag aaaaactctg cggtctacgg catgttgacc
     2761 tcgctgacca ttaccgtcgg gcaaaaggcc gaagagacgg aagcggctga aaaatttgtc
     2821 acctttatgg agcaggcaga caacattgcc gactgggtga tgatgtcgcc aggcgcggcg
     2881 ctgccggtga acaaagcggt tgtcactacc gccacctgga aagacaacga cgttattaag
     2941 gcgctgggtg agctgccaaa tcagttaatc agcgaactgc caaatattca ggtctttggc
     3001 gcagtagggg ataaaaattt tacccgcatg ggcgatgtga cgggttctgg cgtggtgagc
     3061 tccatggtgc ataacgtcac cgtgggtaaa gccgacctcc ctggcacgct gcaaacgagc
     3121 cagaaaaagc tggatgagtt ggttgaacag cgctaaaccc aggacaggaa tccgcaatga
     3181 acaggctttt ttcaggtcgt tccgatatgc cctttgcgct gttgcttctc gcgcccagct
     3241 tattactgct gggcggtctg gtggcgtggc cgatggtgtc gaatatcgaa atcagttttt
     3301 tacgtctgcc gcttaatccc aacatcgagt caacgtttgt tggggtgagc aactacgtgc
     3361 gtattctctc cgatcccggc ttctggcact cgctgtggat gacggtctgg tataccgcgc
     3421 tggtggtggc gggcagcacc gtgctcgggc tggccgtggc gatgtttttc aaccgcgagt
     3481 tccgcctgcg caaaaccgcg cgttcgctgg tgatcctctc ctacgtaacg ccgtctattt
     3541 cgctggtgtt cgcctggaaa tacatgttca acaacggtta cggcattgtg aactacctcg
     3601 gcgtcgatct tctgcatctc tatgagcagg caccgctgtg gttcgataat ccgggcagta
     3661 gctttgtgct ggtggtgctg ttcgccatct ggcgctactt cccctatgcc tttatttcgt
     3721 ttctggcgat tttgcagacc atcgacaaat cgctgtacga agcggcagag atggatggcg
     3781 ctaatgcctg gcaacggttt cgtatcgtca cgctgcccgc gattatgccg gtcctggcga
     3841 cggtggtgac cctgcgcacc atctggatgt tctacatgtt cgcggatgtt tatctgctga
     3901 cgaccaaggt cgatattctc ggtgtctatc tctacaaaac cgcctttgcc tttaatgact
     3961 tagggaaagc ggcggcgatc tcggtggtgc tcttcatcat tattttcgct gtcattctgc
     4021 tgaccagaaa acgggtgaac ctcaatggca acaaataaac gcacactcag tcgcatcggt
     4081 ttttactgcg ggctggcgct gtttctcatc atcacgctgt ttccgttttt tgtgatgctg
     4141 atgacctcgt tcaagagcgc aaaagaggcg atctcgctgc atcctacgct gctgccgcaa
     4201 cagtggacgc tggagcatta tgtcgacatt tttaacccga tgattttccc gtttgtcgac
     4261 tacttccgta acagcatggt ggtgtcggtg gtttcatccg tggtggcggt atttctcggc
     4321 attctcgggg cttacgcgct ttcccgcctg cgctttaaag ggcggatgac catcaacgcc
     4381 agcttttaca cggtgtatat gttctccggc attttgctgg tggtgccgct gttcaaaatc
     4441 atcaccgcgc ttggcatata cgacaccgag atggcgctga tcattaccat ggtaacgcaa
     4501 accctgccca ccgccgtgtt tatgctgaaa agctacttcg acaccatccc tgatgagatc
     4561 gaagaagcgg cgatgatgga tggcctcaac cgtctgcaaa tcatcttccg tatcaccgtg
     4621 cccctggcga tgtccgggct gatatccgta ttcgtctact gctttatggt ggcatggaac
     4681 gactatctgt ttgcgtcgat tttcctctcc agcgccagca atttcacctt accagtcggc
     4741 ctgaacgcgc tgttcagtac gcctgattac atctggggac ggatgatggc ggcctcgctg
     4801 gtgaccgcat tgccggtggt gattatgtat gcgctttcgg agcgttttat taagagtggt
     4861 ttgaccgccg gtggcgtgaa gggctaaagc ggccagtttc tttacaagga gttttacatg
     4921 aaaaagttag tagccacagc accgcgcgta gctgcgctgg ttgagtatga agatcgggcg
     4981 attttagcca atgaagtgaa gatccgcgtg cgcttcggcg caccgaagca cgggacggaa
     5041 gtggtcgatt tccgcgctgc cagtccgttt atcgatgaag actttaacgg cgaatggcag
     5101 atgttcaccc cgcgtccggc agatgcgccg cgcggcattg agtttggcaa attccagctt
     5161 ggcaacatgg tggttggcga cattatcgag tgcggcagcg acgttaccga ctacgcggtg
     5221 ggcgacagcg tatgcggcta cggcccgctc tccgagacgg tcatcattaa cgcagtgaat
     5281 aactacaagc tgcgcaaaat gccgcaaggc agttcgtgga aaaacgctgt ctgctacgac
     5341 ccggcgcagt ttgccatgag cggcgtgcgc gatgccaatg tgcgcgtggg cgattttgtg
     5401 gtggtggtag ggcttggcgc gatcggtcaa attgccattc aactggctaa acgtgctggc
     5461 gcgtcggtgg tgattggcgt cgatcctatc gcccatcgct gtgagattgc ccgtcgccat
     5521 ggcgcggatt tctgccttaa ccccattggc agtgacgtag gcaaagagat caaaacgcta
     5581 accggcaagc agggtgccga tgtgattatc gaaaccagcg gctatgccga cgcgctgcaa
     5641 tcggcgctac gaggtctggc atatggcggc accatctcgt atgtcgcgtt tgccaaaccg
     5701 tttgccgaag gttttaacct cggacgcgaa gcccatttca ataacgccaa aattgttttc
     5761 tcccgtgcgt gcagcgagcc gaacccggat tatccgcgct ggagccgcaa acgtatcgaa
     5821 gaaacctgtt gggaactgct gatgaacggt tatctcaatt gtgaagattt aatcgacccg
     5881 gtggtgacct ttgccaatag cccggaaagt tacatgcagt atgtcgacca gcatccggaa
     5941 cagagcatca aaatgggcgt tacgttttaa ttcaaaggaa tgaaaaaaat gaaaatcggc
     6001 acacagaatc aggcgttctt tccggaaaac attctggaga aatttcgtta tatcaaagag
     6061 atgggtttcg atggttttga gatcgacggc aaactgctgg tcaacaatct cgaagaagtc
     6121 aaagcagcaa tcaaagaaac cggtttaccg gtgatcaccg cctgcggtgg ctatgacggt
     6181 tggattggcg actttatcga agagcgtcga cttaatggct taaagcagat cgaacgcatt
     6241 cttgaagctc tggcagaagt gggcggtaaa ggtatcgtcg ttccggctgc atggggcatg
     6301 tttaccttcc gcttaccgcc gatgacctcg ccgcgtagcc tggacggcga ccgcaaaatg
     6361 gtgagtgatt ccctgcgcgt actggaaaag gtggccgcac gtaccggaac cgtggtgtac
     6421 ctcgaaccgt taaaccgtta tcaggatcac atgatcaaca ctctcgccga tgcccgccgt
     6481 tatatcgtcg aaaacgatct taaacatgta cagattatcg gcgatttcta tcacatgaat
     6541 atcgaagaag ataacctggc gcaggcgctg catgacaacc gcgacctgct cggtcatgtg
     6601 catattgccg ataaccatcg ctaccagccg ggcagcggca ccctggattt ccacgcgctg
     6661 ttagaacagc tgcgcgcgga taactttcag ggctatgtgg tgtacgaagg gcgcatccgg
     6721 gcggaagatc cagcccaggc gtaccgtgat tctttggcct ggttgcgtac ctgctaagag
     6781 gtctttgtga aaagtgcaat gacaagctct ccgctgcggg tcgcgataat aggcgcgggc
     6841 caggtggcgg ataaagttca tgcttcgtac tactgcaccc gcaacgatct ggaactggtg
     6901 gctgtctgtg acagccgcct ttcccaggcg caggcgctgg cagaaaaata cgggaatgca
     6961 tccgtgtggg acgatccgca ggccatgctg ctggcggtga aacctgatgt ggttagcgtc
     7021 tgctcaccta accgttttca ttacgaacat accctgatgg cgctggaagc gggctgccat
     7081 gtgatgtgcg aaaaaccgcc cgccatgacg gcagaacagg cgcgggaaat gtgcgatacc
     7141 gcgcgcaaac agggcaaggt gctggcctac gactttcacc atcgttttgc actcgatacg
     7201 caacagctgc gtgaacaggt gaccaacggc gttttgggag agatttacgt taccaccgcc
     7261 cgcgccctgc gtcgttgcgg tgttcccggc tggggtgtct ttactaataa agaattgcag
     7321 ggcggaggcc cgctgatcga catcggcatt catatgctgg atgcggcgat gtatgtgctg
     7381 ggttttccag cggtgaaaag cgtgactgcg catagctttc aaaagatcgg cacgcaaaag
     7441 agctgcggtc aatttggcga gtgggacccg gcaacctaca gcgtcgaaga ttcgctgttt
     7501 ggcaccattg aatttcataa cggcggcatt ctgtggcttg agacctcatt tgcactcaac
     7561 atccgcgaac agtcgattat gaacgtcagc ttttgtggtg ataaagccgg ggcgacgctg
     7621 tttccagcac atatctacac cgataacaac ggtgagttaa tgacgctgat gcaacgggaa
     7681 atggcagacg acaaccgtca tctgcgcagt atggaagcct ttatcaacca cgtacaaggt
     7741 aagcccgtga tgatagccga cgccgagcag ggatacatca tccagcaact ggtggcggcg
     7801 ctttatcaat ccgcagaaac agggacgcgt gtggaattat gatcaggcca gtaacgttaa
     7861 cggaacccca tttcagccag cataccctga acaagtatgc ttcgctgatg gcgcagggga
     7921 acggctatct tgggcttcgc gccagccatg aagaagatta cacgcgccag acgcgcggca
     7981 tgtatctggc agggctttat catcgggcgg gtaaaggtga aatcaacgaa ctggtgaacc
     8041 tgcctgatgt cgtggggatg gagattgcca taaatggtga gattttctcg ttatcccgcg
     8101 aagcatggca gcgtgagctt aactttgcca gtggcgaatt acgccgcaac gttgtctggt
     8161 gtaccagcaa cggcgcaggt tacaccatca ccagccgtcg ctttgtttcg gcagaccaac
     8221 tgccgctcat tgcgctggaa atcactatta cgccactgga cgccgacgcg ttagtgctga
     8281 tttcaacagg cattgacgcc acgcaaacca accacggtcg ccaacatctc gacgaaaccc
     8341 aggtgcgggt atttggtcag catctgatgc aggggatcta caccacccag gatgggcgca
     8401 gtgatgtggc catcagctgt tgctgtaagg tgagcggtga tgtgcagcaa tgctataccg
     8461 ccaaagagcg ccgtttactg caacatacct gcgcgcagct tcacgcaggt gagacgctga
     8521 cgctgcaaaa acgggtgtgg atcgactggc gggacgacag acaagccgct ttagacgaat
     8581 ggggcagtgc gtcgcttcgt cagcttgaaa tgtgcgcgca gcagagttac gaccaacttc
     8641 ttgcagcatc aacagaaaac tggcgtcaat ggtggcagaa acgtcgtatc acggtaaacg
     8701 gcggcgatgc gcacgatcag caagcgttag attatgcgct ttatcacctg cgcatcatga
     8761 cgcctgccca cgatgagcgc agcagcattg cggcgaaagg tttaaccggc gaaggctaca
     8821 aaggccacgt tttctgggat acagaagtat ttttgctacc gttccatctg tttagcgatc
     8881 cgacggttgc ccgaagttta ctgcgttatc gctggcacaa cttgcctggc gcgcaggaga
     8941 aagcgcgacg caacggctgg cagggcgctc tgtttccgtg ggaaagcgcg cgcagcggcg
     9001 aagaagagac gccggaattt gccgccatta acatccgcac cggactgcgc cagaaagtgg
     9061 cctcggcgca ggctgaacac catttggtgg ctgatatcgc ctgggcggtt attcaatact
     9121 ggcaggcaac gggggatgaa agtttcattg cccacgaagg catggcgtta cttctgggaa
     9181 ccgcaaaatt ctggattagc cgcgctgtac aggtaaacga tcgtctggaa attcacaatg
     9241 ttattgggcc agacgaatat accgaacatg tcaataataa cgccttcacc agctatatgg
     9301 cgtattacaa cgtccagcag gcgctgaata tcgctcgtaa gtttggttgt agcgacgacg
     9361 cgtttattca tcgtgccgaa atattcctca aagaactgcg gctgccagaa attcagcctg
     9421 acggcgtttt gccgcaggat gattcgttta tggcgaaacc agcgattaat ctggcgaaat
     9481 acaaagcggc ggcggggaag caaaccattc tgctggatta ttcacgcgca gaagtgaacg
     9541 agatgcagat cctcaaacaa gctgatgtgg tgatgctcaa ttacatgctg ccggagcagt
     9601 actcagcggc atcgtgtctt gccaatctgc aattttatga accgcgcacc attcacgact
     9661 cttcactgag taaagcgatc cacggcattg ttgccgcacg ctgtggcctg ttggcgcaaa
     9721 gttatcagtt ctggcgcgag gggactgaaa tcgatcttgg tgctgatccg catagctgtg
     9781 atgacggtat ccacgcagcc gcaactggcg ctatttggct gggggcgatt cagggttttg
     9841 ccggggtgag cgtgcgtaac ggtgaattgc atctcaatcc agcgttacct gagcagtggc
     9901 aacagttgtc tttccctctg ttctggcagg gctgcgaatt acaggtcact ctcgacgcgc
     9961 agcgtattgc gattcgaact tctgcgcccg tttcactgcg tttgaacggg cagcttatat
    10021 ctgtggctga agaatctgtt ttctgtttgg gtgattttat tttgcccttc aatgggaccg
    10081 ctaccacgca tcaggaggat gaatgaaact gcaaggggta attttcgatc tggatggtgt
    10141 aatcaccgat accgcgcatc tgcatttcaa ggcgtggcag cagattgccg ctgaaattgg
    10201 catcagcatt gatgcgcagt ttaacgaatc cctaaaaggg atcagccgcg atgagtctct
    10261 gcggcgcatt ctgcaacacg ggggcaaaga gggcgacttt aacccgcagg agagggcgca
    10321 actggcgtat cgcaaaaatc tgctctacgt ccactcactg cgcgaactga cggttaatgc
    10381 tgtgctgccc ggcattcgca atttgctggc agatctccgt gcacagcaga tccctatagg
    10441 gctggcttct gtctccctga atgcgccgac gattttagca gcgctggagc tgcgcgagtt
    10501 tttcaccttc tgcgcggacg cttcccaact taaaaactcg aaaccggacc cggaaatctt
    10561 tctcgccgcc tgtgccggac tgggcgtgcc gccgcaggca tgtatcggca ttgaagatgc
    10621 gcaggcgggc attgacgcca ttaacgccag cggtatgcgc tcggtgggga tcggcgcggg
    10681 cttaaccggg gcgcaattat tgttgccctc aacggactcc ctgacctggc cgcggttatc
    10741 ggccttctgg caaaacgtat agcaaaggaa tcaacatggc tcagctttcg ttacaacata
    10801 ttcaaaaaat ctacgataac caggtgcatg tggtgaagga cttcaacctg gaaattgccg
    10861 ataaagagtt cattgtcttt gtcggtccgt caggctgcgg taaatcgact acgctgcgca
    10921 tgattgccgg gcttgaggag atcagcggcg gcgatctgtt gatcgacggc aaacgaatga
    10981 atgacgttcc ggctaaagag cgcaatatcg cgatggtgtt cctgaactac gcgctgtatc
    11041 cgcatatgac ggtctacgac aacatggcgt ttggcctgaa gatgcaaaaa atcgccaacg
    11101 aggtgattga tgagcgggtg aactgggcgg cgcaaattct cggcctgcgt gagtacctga
    11161 aacgcaagcc gggggcgctt tccggcgggc aacgtcagcg agtggcgctc gggcgggcga
    11221 ttgtgcgcga agcgggcgtg tttttaatgg atgaaccgct ctctaacctg gatgccaagc
    11281 tgcgcgtgca aatgcgcgca gagatcagca agctgcatca gaaactgaac accaccatga
    11341 tctacgtgac ccacgatcag accgaagcga tgaccatggc gacgcggatt gtgattatga
    11401 aggatgggat tgttcagcaa gtaggtgcgc cgaaaacggt ttataaccaa ccggcgaata
    11461 tgtttgttgc cggatttata ggatcaccgg cgatgaattt tattcgcggc acgattgatg
    11521 gcgataaatt cgttacggaa acgcttaaat taaccatttc cgaggagaaa ttagcggttc
    11581 tgaaaacaca ggaaagtttg cataagccca ttgtgatggg aatacgcccg gaagatattc
    11641 atccggacgc gcaagaggaa aataacattt ccgccaaaat tagcgtggca gaattaaccg
    11701 gtgctgaatt tatgctctac accacggttg gggggcacga actggtggtc cgtgctggtg
    11761 cgttaaatga ttatcatgca ggagaaaata tcactattca ttttgatatg actaaatgtc
    11821 atttctttga tgcagaaacg gaaatagcaa ttcgctaaat acagggggaa ggcattcccc
    11881 caggataata caaggaacaa taatgaaaaa attattaccc tgtaccgcac tggtgatgtg
    11941 tgcgggaatg gcctgcgcac aggccgagga aaagaacgac tggcacttta atatcggcgc
    12001 gatgtacgaa atagaaaacg tcgagggtta tggcgaagat atggatgggc tggcggagcc
    12061 ttctgtctat tttaatgccg ccaacgggcc gtggagaatt tctctggcct attatcagga
    12121 agggccggta gattatagcg cgggtaaacg tggaacgtgg tttgatcgcc cggagctgga
    12181 ggtacattat cagttcctcg aaagcgatga tttcagtttc ggtctgactg gtggtttccg
    12241 taattatggt tatcactacg ttgatgaacc gggtaaagac acggcgaata tgcagcgctg
    12301 gaaaattgcg ccagactggg atgtgaaact gactgacgat ttacgtttca acggttggct
    12361 gtcgatgtat aaatttgcca acgatctgaa tacgaccggt tatgctgata cccgtgtcga
    12421 aacggaaacg ggtctgcaat ataccttcaa cgaaacggtt gccttgcgag tgaactatta
    12481 tctcgagcgc ggtttcaata tggatgatag ccgcaataac ggtgagtttt ccacgcaaga
    12541 aattcgcgcc tatttgccgc tgacgctcgg caaccactcg gtgacgccgt atacgcgcat
    12601 tgggctggat cgctggagta actgggactg gcaggatgat attgaacgtg aaggccatga
    12661 ttttaaccgt gtggggttat tttacggtta tgatttccag aatggtcttt ccgtttcgct
    12721 ggaatacgcg tttgagtggc aggatcacga cgaaggcgac agcgataaat tccattatgc
    12781 aggtgtcggc gtaaattact cgttctgata atgggttaaa ttgccggatg cggcgcgagc
    12841 gccaaatccg gcatacattt atttcaacga catatgcgtg gcaacggtaa tattttgtgg
    12901 tgacggtttt ccggcaatta agcggaataa taactcgcag ctttgttgcc ctaactcctg
    12961 cgtcggaaca tcgatgccgc ccggtgcagg cgttaaaata aatgacagcg tttcattgct
    13021 atagcccacc accgctaact gctgcggaat agcaatattt ttctctgctg ccgcacgata
    13081 aatgctcatt aatttcaggc tgtcagtggc aaatacggct tctggcaacg gcgactggct
    13141 taataattgc cgtgcggctt gcaaggctgt ttcatgggta taaccgccgt caacaatcca
    13201 ttcatcacgc actgcaatat tatgcgcagc cagactttgc ttatagccat taacgcggtc
    13261 aaccgagaca tggacatcaa gcggagcatg caggcaggca atattttgat tcccactttc
    13321 aattaacgca tcggtcaacg caatgctgtc gccaaaatta tcggtatcga cagaataaac
    13381 atgggcatat tgaccttcaa ctttgccaat taccaccacc ggaatatcat atttatcgag
    13441 ttgggcaaaa aatgactcat ccgctggcga acttagcata ataatgcctt taatcatttt
    13501 ctgcttaatt ttgctttcgc atttttgtaa gtcttctgcc aggttgtgcg acgtctgcaa
    13561 tatcacgtcg aaaccttctt cttcagcttt ggcggtgatg gcatgtaaaa cttccgagaa
    13621 aaacggatta cccgccgtag ttttggtcga acgggtagaa atcaccataa tggcatcaaa
    13681 acccgaagag gtcagcgcgc gtgccagctt gtttggttga tactgtaatt cttcaatggc
    13741 ccgtaacact ttttcgcgcg cttccgggga gatattggtt tgcttattca gcacgcgtga
    13801 tacggtggat tttgatacgc ctgcaaccct ggcaatatca taaatagtag gggacatagg
    13861 tcaggaactc cgtccgaacg tgaatgttgc acatcttatg gagttcgccc gtcgatgaca
    13921 acacgggcag ttgataatca atggcctggc ccccacattc atatccttac gaatgatttt
    13981 tttctggtct tcagagcagc acaggacagc aatgaagcga cttaaaaatg aacttaatgc
    14041 gctggtgaat cggggtgtcg acagacatct gcgcctcgct gtaaccggac ttagccgcag
    14101 cggcaaaaca gcgtttatca ccgcgatggt taatcagttg ctcaatattc atgccggagc
    14161 acgtttgccg ctattaagtg cggtgcgtga agaacgcctg ctgggcgtaa aacgcattcc
    14221 ccagcgtgac tttggcattc cgcgcttcac ctatgatgaa ggactggcgc agttatatgg
    14281 cgatcctccc gcctggccga cgccaacgcg cggcgtcagc gaaattcgcc tggcgctacg
    14341 ctataaatcg aacgattcgc tgctgcgcca ctttaaggat acctccacgc tgtatttgga
    14401 gattgtggat taccccggcg aatggttgct cgacctgccg atgctggcgc aggactattt
    14461 aagctggtcg cgccagatga cgggcttact caatggtcag cgcggcgaat ggtcggcgaa
    14521 atggcgaatg atgtgcgaag ggctggaccc gctagcgcct gccgacgaaa accggctggc
    14581 agacattgcc gccgcgtgga ccgattatct ccaccactgt aaacagcagg ggctgcactt
    14641 tattcagcct gggcgctttg tcttgccggg agatatggca ggtgcgcccg cgctgcaatt
    14701 cttcccatgg ccagatgtcg atgcctgggg cgagtctaaa ctggcgcagg ccgataaaca
    14761 caccaacgcc ggaatgttgc gcgagcgttt taattattac tgcgagaagg tggtgaaagg
    14821 attctataaa aatcacttcc tgcgttttga ccgccagatt gtgctggtgg actgcctgca
    14881 accgctcaac agtggaccac aggcatttaa tgatatgcgt ctggcgctga cgcagctgat
    14941 gcaaagtttc cactacgggc agcgtaccct gttccggcgt ttgttttcgc cggttatcga
    15001 taagctattg tttgctgcca ctaaagcgga ccatgtgacc atcgatcagc acgctaatat
    15061 ggtttcatta ctgcaacaac tgattcagga tgcctggcaa aatgcagcgt tcgaagggat
    15121 cagcatggac tgcctggggc tggcgtcagt tcaggcgacc accagcggca ttattgatgt
    15181 taacggtgag aaaatcccgg cgctgcgcgg taatcgactt agtgatggcg caccgctcac
    15241 tgtttatcct ggcgaagtcc ccgcacgttt gcctggtcag gcgttctggg ataagcaagg
    15301 cttccagttt gaggcgtttc gtccgcaggt gatggatgtc gacaaaccgc taccgcatat
    15361 tcgccttgat gctgcgctgg aatttttaat aggagataaa ttgcgatgac cgaaccgtta
    15421 aaaccacgta ttgatttcga cggtccactg gaggtcgatc agaatccaaa attcagggcg
    15481 cagcagacct ttgacgaaaa tcaggcgcaa aattttgccc cggccacgct cgacgaagcg
    15541 caggaagaag aggggcaagt tgaagcggtg atggacgcag cgttacgtcc gaaacgcagc
    15601 ctgtggcgca aaatggtgat gggcgggttg gctctgtttg gcgcaagcgt tgtcgggcag
    15661 ggtgtacagt ggacaatgaa cgcctggcaa acccaggact gggtggcgct gggtggatgt
    15721 gccgctgggg cattgattat cggcgctggc gtaggttctg tggtaacaga gtggcggcgc
    15781 ttatggcgct tgcgacagcg cgcccatgaa cgcgacgaag cgcgtgattt attgcatagt
    15841 cacggcacgg gtaaaggccg cgcattttgc gaaaaactgg cacagcaggc gggcatcgat
    15901 cagtcacatc cggcgctgca acgctggtat gcctcaatcc atgaaacgca aaacgaccgt
    15961 gaagtggtca gtttgtatgc gcatttggtc cagccagttt tagatgccca ggcgcggcgc
    16021 gaaatcagcc gttcggcagc ggaatcaaca ttgatgattg cggtcagccc actggcgctg
    16081 gtggatatgg cttttatcgc ctggcgcaat ctgcgtttga tcaatcgcat cgccacgctg
    16141 tatggcattg aactgggata ttacagccgt ttgcgcctgt ttaagctggt attgctgaat
    16201 atcgctttcg ccggagccag cgaactggtg cgcgaagtgg ggatggactg gatgtcgcaa
    16261 gatctcgctg cgcgtttgtc tacccgggca gctcagggga ttggtgctgg acttctgacg
    16321 gcacgactgg ggattaaagc tatggaactt tgccgcccgc tgccgtggat tgaagatgac
    16381 aaacctcgcc tcggggattt ccgtcgtcag cttatcggtc aggtgaaaga gacgctgcaa
    16441 aagggcaaaa cgcccagcga aaaataatgc aatatcgggt gctgaccgga tatctttacg
    16501 ccgaagtgcc cgtttttccg tctttgtgtc aatcattgtt gacagaaacc ttcctgctat
    16561 ccaaatagtg tcatatcatc atattaattg ttcttttttc aggtgaaggt tcccatgcgt
    16621 ctggaagtct tttgtgaaga ccgacttggt ctgacccgcg aattactcga tctactggtg
    16681 ctgagaggca ttgatttacg cggtattgag attgacccca ttgggcgaat ctacctcaat
    16741 tttgccgaac tggaatttga aagtttcagc agtctgatgg ccgaaatacg ccgtattgca
    16801 ggtgttaccg atgtgcgcac tgttccgtgg atgccttccg aacgtgagca tctggcgttg
    16861 agcgcgttac tggaggcgtt gcctgagcct gtgctttctg ttgatatgaa aagcaaagtg
    16921 gatatggcga acccggcgag ctgtcagctt tttgggcaaa aattggatcg cctgcgcaac
    16981 cataccgccg cgcaattaat taacggcttt aattttttac gctggctgga aagcgaaccg
    17041 caagactcgc ataacgagca tgttgttatt aatgggcaga atttcctgat ggagattacg
    17101 cctgtttatc ttcaggatga aaatgatcaa catgttctga ccggtgcggt ggtgatgttg
    17161 cgctcgacga ttcgtatggg ccgtcagttg cagaatgtcg ctgcacagga cgtcagtgcc
    17221 ttcagtcaaa ttgtcgccgt cagcccgaaa atgaagcatg ttgtcgaaca ggcgcagaaa
    17281 ctggcgatgc taagcgcgcc gttgctgatt acgggtgaca ccggtacagg taaagatctc
    17341 tttgcctacg cctgccatca ggcaagccca cgagcgggca aaccttacct ggcgctgaac
    17401 tgcgcgtcta taccggaaga tgcggtcgag agtgaactgt ttggtcatgc tccggaaggt
    17461 aaaaaagggt tctttgagca ggcgaacggt ggttcggtgc tgttagatga gataggcgag
    17521 atgtcgccaa ggatgcaggc gaaattactg cgtttcctca atgatggtac tttccgtcga
    17581 gttggtgaag atcatgaagt gcatgtcgat gtaagggtga tttgcgctac gcagaagaat
    17641 ttggtcgaac tggtgcaaaa aggcgtgttc cgtgaagatc tctattatcg tctgaacgtg
    17701 ttgacgctca atctgccgcc gctacgtgac tgtccgcagg acatcatgcc gttaaccgag
    17761 ctgttcgtcg cccgctttgc cgacgagcag ggcgtgccgc gtccgaaact ggccgctgac
    17821 ctgaatactg tacttacacg ttatgcgtgg ccgggaaatg tgcggcagtt aaagaacgct
    17881 atctatcgcg cactgacaca actggacggt tatgagctgc gtccacagga tattttgttg
    17941 ccggattatg acgccgcaac ggtagccgtg ggcgaagatg cgatggaagg ttcgctggac
    18001 gaaatcacca gtcgttttga acgctcggta ttaacccagc tttatcgcaa ttatcccagc
    18061 acgcgcaaac tggcaaaacg tctcggcgtt tcacataccg cgattgccaa taagttgcgg
    18121 gaatacggtc tgagtcagaa gaagaacgaa gagtaagcgc gaatatgcct gatggtgcaa
    18181 caccatcagg catattaact tatgctttca gtacagccag agctgcttcg taatccggct
    18241 cggtggtgat ttcatccacc agctggctga aaatcacatt gtcattttca tcaataacca
    18301 caacggcacg cgctgccaga cctttcagtg ggccatcagc aattgccaca ccgtaggctt
    18361 gcagaaattc agcgttacgg aaagtggaga gggtgataac gttgttcaga ccttctgcgc
    18421 cgcagaaacg agactgggcg aacggcagat cggcagagat acacagcaca acggtgttgt
    18481 cgatctctgt tgccagttgg ttaaacttac gtactgatgc ggcgcaaaca ccggtatcaa
    18541 tactcgggaa aatgttcagc actttgcgtt tacccgcaaa ctgaccgagg gtgacgtcag
    18601 acagatcttt tgccacgaga gtaaaagtct gcgctttgct acccgcctgc gggatggaat
    18661 tggcgactgt aaccgggttg ccctggaaat gaacggtttg tgacatgatt atctttcctg
    18721 tttacatata gttaacgtca cacctagttt atgctaactg tcaataacac agcaaacgct
    18781 atttgcgctt aatccgcaga ccaccgcgac aacaaggagt aaagatgaga accgttaagg
    18841 tattcgagga aggctggccc ttacataccc cgtttgtgat tgctcgggga agtcgcagtg
    18901 aagcgcgcgt ggtggtggtt gaactggaag aagaaggtat taaaggcacc ggtgaatgca
    18961 cgccgtatcc gcgttatgga gaaagtgatg cctcggtaat ggcgcaaatt atgagcgtcg
    19021 tgccgcaatt agagaaaggg ctgacacggg aggagttgca aaaaatcctc cctgccggcg
    19081 cagcacgtaa tgcgctggat tgtgcattgt gggatctcgc ggcgcgaaaa cagcaggaat
    19141 cgctggctga tttgatcggc ataacgcttc ccgggacagt tatcactgca cagacggttg
    19201 tcatcggtac gcctgatcag atggccaata gtgcatcaac actctggcag gcaggcgcga
    19261 aattactgaa agtgaagctg gataaccatc ttatcagtga gcggatggtg gcaattcgca
    19321 ccgctgtgcc cgatgcgacg ctgatcgttg atgcaaatga atcctggcgt gcagaagggt
    19381 tggcggcgcg ttgccagcta ttggcggatt taggcgttgc aatgcttgaa caaccgcttc
    19441 ctgcgcagga cgatgcggcg ttggagaatt ttattcatcc gttaccgatt tgtgctgatg
    19501 aaagttgtca tactcgtagc aatctgaagg cgctgaaagg gcgctatgag atggttaaca
    19561 ttaagctcga taaaacgggg ggcctgacgg aagcgctggc gctggcgact gaagcacgtg
    19621 cacaaggttt tagtctgatg ctgggctgca tgttgtgtac ctctcgtgcc attagcgccg
    19681 ctttaccgct ggtgccgcag gtcagtttcg ccgatcttga cggaccgacc tggctggcgg
    19741 tagatgtgga accggcgctt cagttcacaa cgggcgaatt gcatctttag gatgccagcg
    19801 cagcaagttt gccatcgcaa agagatattt ttcgctggct tcgtcggagg agatgggtgg
    19861 aaactctgcg gtgatgcaat gcaggttgag atccgcacac caactgccga aagagccagg
    19921 cgtttcataa ccgacgctgg ttaccagagg caattcaaac gcctgggcca gccattcacc
    19981 taattcgctg tgtctgggat cttcaataca ggccagcgga tcgtggaatg agaccaccca
    20041 ggcgggttga atgcggtgta taagctggca cagcgcctgg gtttcaggtt cggagccagg
    20101 tttgtcacct gtcagtaaaa cgacatcacg ttcttcagcg gcgctgttcc agcgataaac
    20161 ggtttcacct tccttccagt ttgccgccgg aaagtttctg tttaaatcca caccattagc
    20221 attggccctt aaccccaact ggcagccgtc aggattcaca cacagcacca catgatgacg
    20281 gcgcaaagaa ggtgtcaacg tccgcagagc acaggagagc gtgacgatcg aagagttttc
    20341 atcaccgtga gtgccagcca gaatcaaacc actttcgtga ctggctgcag gggccggaaa
    20401 ccagattaac ggcgcgccca ataatgaacg tccgtaatgt tctgttccgg gcggaaatgc
    20461 gccgcgttcg gcgcgtgggc gagttacggt catgatcgtc tctggttcag gaagtattac
    20521 aggcagtgtt gtgcaaattt tgcggattat caaatccttt tcttcaacgc tatgttatcg
    20581 atcttgtatc acttttttgc cggaagttgt ttgcattttc attgatgtgc agatattaga
    20641 attgtcgtaa tgttatatac atggcgtatt ttattcttac tggttctaca catgttaatc
    20701 gcatctgatt gatcagttca ccttaataag tttaataaat cgtactgcac atagtacagt
    20761 acgatggtag ctatataatt gatttacatg aagaatggct tatcgccatc agtcccgggt
    20821 tgattgattc ctatattctc accaatgtgt ttctcaggtt tatcgatgat atctgtaatt
    20881 aatgctgcga cactcttacg tgatacgatc gtacctttga atggttcatt gcgtgaggta
    20941 agctcataat caataatatc ttcatcagtc agccaggcag ggcgaagaat tgtatattcc
    21001 agtccagaag cctcaattgc atctgcagcc ctgcggaatg gttttagggg ttcaccaata
    21061 actgcattat tccattcgcc aaattttcct ggaacttcat cataaatacc gagggaaaga
    21121 acaaagatca gacgtttaac atcaccggct ttcatcgcag cgatcactga atttgcctga
    21181 atatctaaat cttcccccgt aaggttggca tacacaacat cctgtccctg catggcctgc
    21241 ttcagtgctg catgattcag tacatccccc ataataatct tgctattagt gggatagggt
    21301 ttgtggattt ttgctggctg acgggcaaat aaagtttgtt taatggtttg tttatctgcc
    21361 agttgattaa tcacatggcg ggctatctga ccgccagcac caagaatcag tacatttttc
    21421 atgaaacttt tcccagatta ttggcagcaa tatgccaggc catatcacta ttacggattt
    21481 gaatatgttt cattacagag tttcctcaaa gaacggagct aatactgaaa ttgcttcagc
    21541 cacatagact ttgccgtcat aaagatccat atggtttgcg ccttcaacaa tgtgatagcg
    21601 tttatcctgg cttgatgctc gatcgtacag gtcgtcactc atccatttgc tccctgcctg
    21661 gctgcccgcc acaatctgta tcggctgagt caggtacact tccgccatat ggtaagcatc
    21721 ataggtaata atctggttaa ggctgcgcaa agtagcgtaa cccggtgctg ttggatactg
    21781 cgcgcgaggg gtatggtaat actcccaggc ctgacgcagt tcttcgttcg gcgcatcgga
    21841 ctccttcatt ggtgccagtg gcatagtggc gtattctctg ctgctaatat cgctggttcg
    21901 ggcgtttgaa cccgcgtcaa cgtatggtag ggcatctata gatttcacat tgttttccca
    21961 accattacgg aacatcgaac caatattgac cgcactaaca gtaccgatgg ccttgatgcg
    22021 gcgatcctga attgcagcat tggctgtata tcctgcaccg gcacaaattc ccatcgcacc
    22081 aattcggcta ttgtcgacat aagaaagcgt tgtcaggtaa tcaatcacgg cactgacgtc
    22141 ttcagtacga atgtatgggt tttctaactg acgcggctcg ccgccgcttt caccttgata
    22201 agatgcgtca taagcaatag tgacaaatcc cttttccgcc agtttttcgg cataggttcc
    22261 agccgtttgc tctttaacgc ccccacctgg gtgagatacc acaattgccg gatactgacg
    22321 ggtttcatca aattttggag ggaaatagat cactgcagac aaagagatgg ttggattatt
    22381 gctgttagtg aagctgactt tattattcat cattccgttc ctcatggagt tgtcggttcg
    22441 ttttgacggt tggtgatatc actatagata ttgatcatta agttgattag acccaaaatc
    22501 atgattagac ctatcattta aatgattaat agattgtatg gagatactga atgaagcgtg
    22561 aagaaatcgc tgatctgatg gcgtttgtcg tcgttgcaga ggagcgtagc ttcactcgcg
    22621 cagcagcccg cctgagcatg gcgcagtcag ctttaagcca gatagtgcgc cgtatagaag
    22681 aacgattggg attgcggctt ctgacacgaa ccacgcgcag cgttgtaccc actgaagcgg
    22741 gcgagcatct tttgtctgtt cttggcccta tgttgcatga catagattct gccctgacat
    22801 ccctgagcga tctgcagaac cgcccatccg ggacaatacg tattactact gtagaacatg
    22861 cagcaaaaac gatattgtta ccagctatgc gcacattcct gaaatcgcat cctgaaattg
    22921 atatccagct taccattgat tatggtttga ccgatgtcgt ttctgaacgt tttgatgcag
    22981 gcgtccgtct gggtggggag atggataaag atatgatcgc cattcgaatc gggccagata
    23041 taccaatggc tattgttggc tcaccggatt atttttctcg tcgaagtgct ccaacctcag
    23101 tgtcacaatt aatagatcat caggcaatta atttgtatct tcccacatcg ggtacagcaa
    23161 atcgctggag attaatacgc ggtggacgtg aagttcgtgt tcgcatggaa ggtcagcttt
    23221 tactgaatac gatagacctg atcattgatg ctgcaattga tgggcatgga ttggcgtatc
    23281 taccttatga tcaggttgag cgggctatta aagaaaaaaa actgatacgt gttttggata
    23341 aattcacacc agatttaccc ggttatcacc tgtactatcc acaccgtcga catgctggct
    23401 cggcattctc attatttata gataggctga agtataaagg tgctgtttag cactacttgc
    23461 tgatacatta atttaattct tctcttaacg tattctcagt tcctttcaac gttttggtta
    23521 ttttttattc ttcgtacaat ggcgacagat gctgattatg ataccgaaaa cgggtttgaa
    23581 cgtgcgaagc ccgaacgtag tgttcggagt tctatgtgct ttaccgcatt ttggagacta
    23641 ttatttacac taaatctgat ttgatatatt gatacttaaa acatttgatg cttcctttgt
    23701 cacttttttg atggaagttg tttgcatttc tttaaggcga aacaaataat tacgcatcaa
    23761 ttttaatgtc ggttagaggg aaacttatga agcactctgt ttcagtcacg tgttgtgcgc
    23821 tgttggtcag cagcatttct ctttcgtatg ctgctgaagt tccgagcggc acagtactgg
    23881 cagagaagca ggagctggtg cgccacatta aagatgagcc tgcgtcgctg gatcccgcta
    23941 aagccgtggg cctgccagag attcaggtca ttcgcgatct gtttgaagga ctggtgaatc
    24001 agaacgagaa aggggagatt gtccccggcg ttgcgactca gtggaaaagt aatgacaacc
    24061 gtatctggac ttttaccctg cgcgataacg caaaatgggc ggatggcaca ccggtaacgg
    24121 cgcaagattt tgtctacagc tggcaacgtc tggtggaccc aaaaacattg tcgccatttg
    24181 catggtttgc cgcgctggcg ggaatcaaca acgcacaggc gattattgat ggtaaagccg
    24241 cgcctgacca gcttggcgtc accgcagttg atgcccaaac tttgaaaatc cagcttgata
    24301 aaccgttgcc gtggtttgtg aatttaaccg ctaactttgc cttcttcccg gtgcaaaaag
    24361 ccaacgtaga aagcagtaaa gagtggacga aacccggaaa tctgatcggt aatggcgctt
    24421 atgttcttaa agagcgcgta gtcaatgaaa aactggtcgt ggtaccgaat acccattatt
    24481 gggataacgc caaaacggtg ctgcaaaaag tgaccttcct gccaattaat caggaatccg
    24541 cagccactaa gcgttatctc gcgggggata ttgatatcac cgaatccttc ccgaaaaata
    24601 tgtatcagaa gctgttgaag gatattccgg ggcaggttta tacgccgcca cagctcggga
    24661 cctattatta tgcgtttaac acgcaaaaag ggccgacggc cgatcagcgt gttcgcctgg
    24721 cattaagtat gacgatagat cgccgcctga tgaccgaaaa agtgttggga acgggcgaaa
    24781 agccagcgtg gcattttaca ccagatgtta ctgcaggatt tacgccggaa ccttcgccgt
    24841 ttgaacaaat gagtcaggaa gaactgaatg cgcaggcaaa aactttgttg agcgcagctg
    24901 gttatggtcc gcaaaaaccg ctgaagctga cgcttttgta taacacttca gaaaaccatc
    24961 aaaaaattgc gattgctgta gcatcaatgt ggaaaaagaa ccttggcgta gatgttaaat
    25021 tgcaaaatca ggaatggaaa acctatatcg atagccgtaa caccggcaat tttgatgtta
    25081 tccgagcctc gtgggtaggg gattataatg aaccctccac tttcctgaca ttattaacgt
    25141 caacgcattc aggaaatatt tcacgcttta acaatccggt atacgacaaa gtactggccc
    25201 aggcatcaac ggaaaatacc gttaaagcgc gtaatgccga ttacaatacg gcagaaaaaa
    25261 tcctcatgga gcaagcaccg attgcaccaa tttatcaata taccaatgga cgattaatca
    25321 agccgtggct gaaaggttat cccattaata acccggaaga tgtggcgtac agtcggacta
    25381 tgtatattgt gaagcattga tgtgatggga actggcgtta cccttgtgga taacgccagt
    25441 gatgtctgat ttagcgaccc tggtccggcg gtgtaatgtt atccatatac agcgtctggc
    25501 tggggaaggc aaagtccgcg ccgtgtgact gtacaatatc gataatcttc aaataaacgt
    25561 cttgctgtgc agcaagccat tcagcccata ccgtggtttt ggtaaagcaa taaaccataa
    25621 tattcaatga agagtcagca aactggttga aataaaccag taaggtttgt cgctggtcga
    25681 tggccgggtg atttttcagc atctcacgta cggcttcgac aataacgccc acttttgccg
    25741 catcctcata acgtaaacca atggtcgtgg taatgcgccg gttggtcatt cgtcctgggt
    25801 tttctacgct gatcgacgaa aacagcgagt tcggtacgta caacggacga ttatcaaaag
    25861 tcgtaatttt ggtaattcgc cagccaattt ccgctactgt accttcgata tttctgtccg
    25921 gtgaacggat ccagtcgcca atactaaaag gacggtcgaa atagagcata atcccggaaa
    25981 agaagttact caaaatatct ttaccggcca taccgacagc cagaccacca ataccaccaa
    26041 aggtcaacaa gccagaaagg ctcataccga aatgttcacc ataaagcaga acaagcacca
    26101 caataatggt gattttgatg atacgcgaca taatccgcgc actggtgata tcgcgacctt
    26161 ttttaatctg ctgtttttca aactgattta tcagcagaaa tagcttaatc gtcagaataa
    26221 ccgcaatcag ggacgtacag ataaaatcga taacgcctgg ggtgataaat ttgagtttat
    26281 agttttctat aacataatta atgatgctac caacagcact gataattatg gtgtagatta
    26341 aaaattgcac cgcatggaat aaaaatcctt ttcttttacg atttccgcgg cgaaaccaaa
    26401 agctcatcag aatcaatgct gcgcagctac cgaaaataat gaccagatta agcgcattat
    26461 ttgtaaacag ttcagcgatc attgttttat caggctcctc cagataattg tcgtcatgcc
    26521 ggaaacccct ggcggggcta ttttaccgcg acaattcatt cagatcatca atagtcaggg
    26581 aaggaagtag caacattagc taactaatca atgcgttgat tgtaaatcca gctaagaggt
    26641 gaggttttca gagcagacaa cggtgaaatg tcatggtatt gttacgttta ggtaacaaga
    26701 aatttgtctg cacaaggatt acatcatgat tatggcgaaa ctgaagtcag cgaaagggaa
    26761 gaaatttctc tttggtttgt tggcggtttt cattattgcg gcgtcggttg tgactcgcgc
    26821 gaccatcggc ggcgtgatag aacagtacaa tattccgctg tctgagtgga cgacatcaat
    26881 gtatgtgatt cagtcatcga tgatttttgt ttatagcctg gtctttactg tgttgctggc
    26941 aatcccgttg ggaatttatt tccttggcgg cgaagagcag taagtaaaaa ataggcccga
    27001 taactcgggc cttgtcagtt attgaagagt cgttaatcgt cttcttcgtc atccagttca
    27061 acgggtgtct gatactggtc aggtttaatg accagcaggt cgcagcgaag atgatcaatc
    27121 acctgttccg ccgtgttacc aaggaatgct gctgaaatac cggtgcgtcc gaccgtgccc
    27181 agaaccacaa tccccgcctg taagtgctcc gccaaatcag gaatcacctc ttctggcaga
    27241 cctttttcta cgtgcgtcat gttttcatta atgccgaatt tctgccgcag ggctttcatt
    27301 gccagcaaat gttgcccacg aatggcatcg ttataaacgc tcggatcaaa ttccggcagt
    27361 tcaatcgcga tattaattgg cgttaccgga taagcgccaa ccagatgaac ttcggtatgg
    27421 ttgacttgtt ccgccagttc gatcgtctct ttgaccagtt tttcattgag cgcattatga
    27481 tacggctctt cactggcgag attcaccgcc accagcgcct tacccccttc cggccacggc
    27541 tggtctttca ccatccacac ggggcttggg catttgcgta acagatgcca gtccgttggc
    27601 gtaaaaatca ccgcttccag acggtcatgt tggtgcgcca tttttagcac caaatcgtgc
    27661 ccgccgctga tcacttcctg aatgatggct tcgaaaggac ggttatgcca gaccacttta
    27721 atttcaatgg gaacgccagc attgagataa tattttgcct gctcgtggat ccaggctgta
    27781 cgctggctga tgacgccctg acgcatagcg gtacgttcgt ccggggagag cagggtggtc
    27841 atttcgtatg agaagtcata gatcggcaaa aaggctttaa ttttgccacc aatccgttga
    27901 tgtaaataaa cagctcgccg caatgctggt tggtcgtcct ggttaggatc gataacaacg
    27961 agcatgttct gatacatagc catacagggt ctccttacaa caactgtcaa cgcagtttgt
    28021 aattaaaaga ttaacccata tctggtgaat gaaacagtga tgaaccttct gccagatcaa
    28081 taaatcagaa aaatttaatg atatgacaga aggatagtga gttatgcgga aaaatcaggc
    28141 aacgttacgc gtatgaccag caagctgggc cagcgcatcg ttattttcga tggtgatgta
    28201 tttacctttg actgccagca taccgctttt ctggaagcga cccagcagac ggctgatggt
    28261 ttctaccgtc aggcccagat agttaccaat atcgccacga gtcatcgtca ggcggaattc
    28321 acgaggggag aagccgcgtt gggcaaaacg acgggacagg ttgtagatga atgcagccag
    28381 acgttcctcg gcatttttct tcgacaacag caggatcatg tcctgatcgc ctttgatttc
    28441 accgctcatc agacgcatca tctgctgacg cagattcggc attttaccgg acaaatcgtc
    28501 cagcgtttcg aacgggattt cacataccat cgaggtttcc agcgcctgcg cgaagctcgg
    28561 gtgatggccg ctgccgatgg cgtcaaatcc caccaggtcg cctgctaaat ggaaaccagt
    28621 gatttgctcg tcgccttgct cagtgatggt ataactttta atcgtaccgg agcggatggc
    28681 ataaagcgat ttaagttcat caccagcctt aaacagcgtc tggcctttct gaataggctt
    28741 cttccgctca atgatattat caagctgatc aagctcatgt tcgttgagtg tgaacgggat
    28801 gcaaagctgg ctgatgctgc aatcctggca atggatagca caaccgccag actgaatgcg
    28861 ccgtataatt cgcttttccg ggatcatagg tctgctcaag ccgtaattga tatt
//
LOCUS       DAZGQL010000070        28487 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.070, whole genome shotgun sequence.
ACCESSION   DAZGQL010000070 DAZGQL010000000
VERSION     DAZGQL010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28487
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.070"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..593)
                     /locus_tag="ACHKIR_003303"
     CDS             complement(<1..593)
                     /locus_tag="ACHKIR_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFO9990990.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPPRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQ
                     YDFPGDDTPIVRGSALKALEGDAEWEAKILELAGFLDS"
     gene            complement(664..2778)
                     /gene="fusA"
                     /locus_tag="ACHKIR_003304"
     CDS             complement(664..2778)
                     /gene="fusA"
                     /locus_tag="ACHKIR_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFO9990991.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEEEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2875..3345)
                     /gene="rpsG"
                     /locus_tag="ACHKIR_003305"
     CDS             complement(2875..3345)
                     /gene="rpsG"
                     /locus_tag="ACHKIR_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFO9990992.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3442..3816)
                     /gene="rpsL"
                     /locus_tag="ACHKIR_003306"
     CDS             complement(3442..3816)
                     /gene="rpsL"
                     /locus_tag="ACHKIR_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFO9990993.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3942..4229)
                     /gene="tusB"
                     /locus_tag="ACHKIR_003307"
     CDS             complement(3942..4229)
                     /gene="tusB"
                     /locus_tag="ACHKIR_003307"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HFO9990994.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLAGQISNDIILIDYTDFVRLTVKHSSQMAW"
     gene            complement(4237..4596)
                     /gene="tusC"
                     /locus_tag="ACHKIR_003308"
     CDS             complement(4237..4596)
                     /gene="tusC"
                     /locus_tag="ACHKIR_003308"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HFO9990995.1"
                     /translation="MKRIAFVFSTVPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLPGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4596..4982)
                     /gene="tusD"
                     /locus_tag="ACHKIR_003309"
     CDS             complement(4596..4982)
                     /gene="tusD"
                     /locus_tag="ACHKIR_003309"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HFO9990996.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHELSSVFFYREGV
                     YNANQLTSPASDEFDLVRCWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4982..5704)
                     /gene="yheO"
                     /locus_tag="ACHKIR_003310"
     CDS             complement(4982..5704)
                     /gene="yheO"
                     /locus_tag="ACHKIR_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO9990997.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5871..6683)
                     /gene="fkpA"
                     /locus_tag="ACHKIR_003311"
     CDS             complement(5871..6683)
                     /gene="fkpA"
                     /locus_tag="ACHKIR_003311"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFO9990998.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6904..7122
                     /gene="slyX"
                     /locus_tag="ACHKIR_003312"
     CDS             6904..7122
                     /gene="slyX"
                     /locus_tag="ACHKIR_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HFO9990999.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(7171..7761)
                     /gene="slyD"
                     /locus_tag="ACHKIR_003313"
     CDS             complement(7171..7761)
                     /gene="slyD"
                     /locus_tag="ACHKIR_003313"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFO9991000.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7856..8056)
                     /gene="yheV"
                     /locus_tag="ACHKIR_003314"
     CDS             complement(7856..8056)
                     /gene="yheV"
                     /locus_tag="ACHKIR_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative zinc ribbon protein"
                     /protein_id="HFO9991001.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(8066..9871)
                     /gene="kefB"
                     /locus_tag="ACHKIR_003315"
     CDS             complement(8066..9871)
                     /gene="kefB"
                     /locus_tag="ACHKIR_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HFO9991002.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVTVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9871..10422)
                     /gene="kefG"
                     /locus_tag="ACHKIR_003316"
     CDS             complement(9871..10422)
                     /gene="kefG"
                     /locus_tag="ACHKIR_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HFO9991003.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRSVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     QELASHARAYGDWLANPLSPGGR"
     gene            10553..12466
                     /gene="yheS"
                     /locus_tag="ACHKIR_003317"
     CDS             10553..12466
                     /gene="yheS"
                     /locus_tag="ACHKIR_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO9991004.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12466..13488
                     /gene="yheT"
                     /locus_tag="ACHKIR_003318"
     CDS             12466..13488
                     /gene="yheT"
                     /locus_tag="ACHKIR_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFO9991005.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRKVKFT
                     PHWQRLELPDGDFVDLAWSEDPAQARHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNNLPIDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13482..13700
                     /gene="yheU"
                     /locus_tag="ACHKIR_003319"
     CDS             13482..13700
                     /gene="yheU"
                     /locus_tag="ACHKIR_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HFO9991006.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13754..14623
                     /gene="prkB"
                     /locus_tag="ACHKIR_003320"
     CDS             13754..14623
                     /gene="prkB"
                     /locus_tag="ACHKIR_003320"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HFO9991007.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14678..15082)
                     /gene="yhfA"
                     /locus_tag="ACHKIR_003321"
     CDS             complement(14678..15082)
                     /gene="yhfA"
                     /locus_tag="ACHKIR_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HFO9991008.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15384..16016
                     /gene="crp"
                     /locus_tag="ACHKIR_003322"
     CDS             15384..16016
                     /gene="crp"
                     /locus_tag="ACHKIR_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HFO9991009.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            16067..18157
                     /gene="yhfK"
                     /locus_tag="ACHKIR_003323"
     CDS             16067..18157
                     /gene="yhfK"
                     /locus_tag="ACHKIR_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccS/YhfK family putative transporter"
                     /protein_id="HFO9991010.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQVLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQDAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(18224..19444)
                     /gene="argD"
                     /locus_tag="ACHKIR_003324"
                     /gene_synonym="dapC"
     CDS             complement(18224..19444)
                     /gene="argD"
                     /locus_tag="ACHKIR_003324"
                     /gene_synonym="dapC"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HFO9991011.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQYKGRARDFLYAGAEEGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MHRFAHAVAKVIGA"
     gene            complement(19530..20093)
                     /gene="pabA"
                     /locus_tag="ACHKIR_003325"
     CDS             complement(19530..20093)
                     /gene="pabA"
                     /locus_tag="ACHKIR_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HFO9991012.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHNGVGVFKGLANPLTVTRYHSLVVEPDSLPECFEVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(20125..20727)
                     /gene="fic"
                     /locus_tag="ACHKIR_003326"
     CDS             complement(20125..20727)
                     /gene="fic"
                     /locus_tag="ACHKIR_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HFO9991013.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTLHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQNGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20717..20884)
                     /gene="yhfG"
                     /locus_tag="ACHKIR_003327"
     CDS             complement(20717..20884)
                     /gene="yhfG"
                     /locus_tag="ACHKIR_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HFO9991014.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEALRRHYER"
     gene            complement(20989..21561)
                     /gene="ppiA"
                     /locus_tag="ACHKIR_003328"
                     /gene_synonym="rotA"
     CDS             complement(20989..21561)
                     /gene="ppiA"
                     /locus_tag="ACHKIR_003328"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HFO9991015.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNMTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21832..23013
                     /gene="tsgA"
                     /locus_tag="ACHKIR_003329"
     CDS             21832..23013
                     /gene="tsgA"
                     /locus_tag="ACHKIR_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HFO9991016.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSAA
                     MFILGVVSGITMSIGTFLVTQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILIV
                     LAGLAAILMYVFNTGTPAYMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            23275..25818
                     /gene="nirB"
                     /locus_tag="ACHKIR_003330"
     CDS             23275..25818
                     /gene="nirB"
                     /locus_tag="ACHKIR_003330"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HFO9991017.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHANLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25815..26141
                     /gene="nirD"
                     /locus_tag="ACHKIR_003331"
     CDS             25815..26141
                     /gene="nirD"
                     /locus_tag="ACHKIR_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HFO9991018.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            26268..27074
                     /gene="nirC"
                     /locus_tag="ACHKIR_003332"
     CDS             26268..27074
                     /gene="nirC"
                     /locus_tag="ACHKIR_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HFO9991019.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSIRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGTISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            27093..28466
                     /gene="cysG"
                     /locus_tag="ACHKIR_003333"
     CDS             27093..28466
                     /gene="cysG"
                     /locus_tag="ACHKIR_003333"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HFO9991020.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDALNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMGERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGQVVGLRDK
                     LNWFSNH"
BASE COUNT         6895 a         7664 c         7210 g         6718 t
ORIGIN      
        1 gaatccagga agccagccag ttccaggatt ttcgcttccc actctgcgtc gccttccagc
       61 gctttcagag cagaaccacg aacgatcgga gtgtcgtcgc ccgggaagtc gtactgagac
      121 agaagttcac gaacttccat ttcaaccagt tccagcagct cttcgtcatc aaccatgtca
      181 catttgttca ggaacacgat gatgtacgga acgcctacct gacgacccag caggatgtgc
      241 tcacgagtct gcggcatcgg gccgtcagtc gcagcaacta ccaggatcgc gccgtccatc
      301 tgagcagcac cggtgatcat gtttttaaca tagtcggcgt gccccgggca gtctacgtgt
      361 gcgtagtgac ggggcggggt gtcgtattca acgtgagaag tgttgatggt gataccacga
      421 gctttttctt ccggcgcgtt atcgatctgg tcgaatgcac gagcagcacc gccgtaggtt
      481 ttagccagta cggtggtgat tgcagcggtc agagtagttt taccgtggtc aacgtggccg
      541 atagtaccaa cgttaacgtg cggttttgta cgttcaaatt tttctttaga cacggctata
      601 ttccttacta tagtgctctc cccttcagga gagagcacgg gactttggta ttaaccctta
      661 ggcttattta ccacgggctt caattacggc ctgagcaacg ttactcggcg cttcatcata
      721 cttcaggaat tccatggtgt atgatgcacg acctttggtc agagaacgca gctgagttgc
      781 atatccgaac atttcagaca gcggtacttc agcgtggatc ttaacgccag taacttcaga
      841 ttcctgacct ttgagcatac cacgacgacg gctcaagtca ccgataacgt caccggtgtt
      901 ctcttccgga gtttctactt caaccttcat gatcggctca agcagaactg gtttcgcttt
      961 cttaaagcct tctttaaagg cgatagaagc agccagttta aacgccagtt cagaggagtc
     1021 aacgtcatgg taagaaccga agtgcagacg aatacccatg tctactaccg ggtagcctgc
     1081 cagcggacct gctttcagct gttcctggat acctttatca acggccggga tgtattcgcc
     1141 agggattaca ccacctttaa tgtcgttgat gaactcgtag cctttcgggt ttgaacccgg
     1201 ctccagcggg tacatgtcga taacaacatg accatactga ccacgaccac cagactgttt
     1261 cgcgtgttta ccttcaacat cggtaacttt ctggcggata gtttcacggt aagcaacctg
     1321 cggtttacct acgttcgctt caacgttgaa ttcacgcttc atacggtcaa cgatgatgtc
     1381 gaggtgcagt tcgcccatac ccgcgatgat ggtctggtta gattcttcgt cagtccatac
     1441 acggaaagac gggtcttctt tagccagacg gcccagagcc agacccattt tttcctggtc
     1501 agctttggtt ttcggttcaa ctgcgatgga gattaccggc tcagggaatt ccatacgttc
     1561 cagaatgatc ggcgcatccg ggtcacacag ggtgtcacca gtggttacgt ctttcagacc
     1621 gatagcagca gcgatgtcgc ccgcgcgaac ttctttgatc tcttcacgtt tgttagcgtg
     1681 catctgaacg atacgaccga aacgctcacg tgcagctttc acggagttca gtacggtatc
     1741 accagagtta accacaccgg agtaaacacg gaagaaggtc aggttaccaa caaacgggtc
     1801 ggtagcgatt ttgaacgcca gtgcagagaa cggctcgtca tcacttgcgt gacgttcagc
     1861 cggagtgtct ttaccgtcgt ccaggatacc gttgatcgca ggtacgtcaa ccggggatgg
     1921 caggtaatcg attaccgcat ccagcatcgc ctgaacacct ttgttcttga acgcagaacc
     1981 acaggttacc aggatgattt cgttgttcag aacgcgctga cgcagagcac ctttaatctc
     2041 ttcttcagtc agttcttcac cacccaggta tttttccatc agctcttcag aagcttcagc
     2101 tgcggattcg atcaggttct ggtgccattc gttagccagt tcaaccatgt ctgccgggat
     2161 atcttcgtat tcgaaggtta cgccctggtc agcgtcgttc cagttgatag ctttcatttt
     2221 caccaggtca acaacaccgg tgaaatgttc ttcagcacca atcgccagct gcagcggaac
     2281 cgggttcgcg cccagacggg ttttgatctg gttaacaact ttcaggaagt tcgcacccat
     2341 gcggtccatt ttgttaacga acgcaatgcg cggaacttta tatttgtttg cctgacgcca
     2401 tacggtttca gactgcggct gaacaccacc aactgcgcag taaaccatta ccgcaccatc
     2461 gagaacacgc atggaacgtt ctacttcgat tgtgaagtca acgtgccccg gggtgtcgat
     2521 gatgttgatg cgatgcggct catactgctt agccatacca gaccagaatg cagtagtcgc
     2581 agcggaagtg atggtaatac cacgttcctg ctcctgctcc atccagtcca tggttgcagc
     2641 gccgtcatga acttcaccga ttttatggtt tacaccggtg tagaacagaa tacgttcggt
     2701 agtagtggtt ttaccggcgt cgatgtgcgc actgataccg atgttacggt agcgtgcgat
     2761 gggtgttgta cgagccattt gtttcctcgt ttatctttta ggcgttcaat ttaagtagcc
     2821 caaagcgggc tgcttactgg aagcgcccgc ctggtgacta aaactccgaa gggattacca
     2881 acggtagtgt gcgaacgcct tgttggcttc ggccatacgg tgaacgtctt cacgtttctt
     2941 aactgcagta cctttgtttt ctgcagcatc agaaagttcg ttcgccaggc gcagagccat
     3001 ggatttatca ccgcgtttac gagcagcttc aacgatccaa cgcattgcca gagcattacg
     3061 acgaaccgga cggacttcaa ctggtacctg ataagtagaa ccaccaacgc ggcgagactt
     3121 aacttctaca gtcgggcgca cgttttcgag agctacttcg aatgcttcca gttcagattt
     3181 accagagcgc tgagccaggg tctccagcgc gctgtatacg atagattcag cagtagattt
     3241 tttaccatct accatcagga tatttacaaa tttagccagc agttctgatc cgaacttcgg
     3301 atccggcaga attttacgct gaccaatgac gcgacgacgt ggcatggaaa tactccgttg
     3361 ttaattcagg attgtccaaa actctacgag tttagtttga catttaagtt aaaacgtttg
     3421 gccttactta acggagaacc attaagcctt aggacgcttc acgccatact tggaacgagc
     3481 ctgcttacgg tctttaacgc cggagcagtc aagcgcacca cgtacggtgt ggtaacgaac
     3541 acccgggagg tctttaacac gaccgccacg gatcaggatc acggagtgct cctgcaggtt
     3601 gtgaccttca ccaccgatgt aggaagtcac ttcgaaaccg ttagtcagac gaacacggca
     3661 tactttacgc agcgcggagt tcggtttttt aggagtggta gtatatacac gagtacatac
     3721 gccacgtttt tgcgggcatg cttccagcgc aggcacgttg cttttcgcaa ctttgcgagc
     3781 acgtggtttg cgtaccagct ggttaactgt tgccattaaa tagctcctgg ttttagcttt
     3841 tgcttcgtaa acacgtaata aaacgtcctc acacaatatg aggacgccga attttagggc
     3901 gatgccgaaa aggtgtcaag aaatatacaa cgatcccgcc atcaccaggc catctggctg
     3961 gagtgcttaa ccgtaagtct gacgaaatca gtatagtcaa tgagaatgat atcgttcgaa
     4021 atttgaccag ccaaaccgcg ggcaataagg tcttcgttca gggcatagac cttaatgggg
     4081 gcattacgca gactttcaag gtagcggtta ccgtcaactg cggcagttac accatcttgc
     4141 aataatagca gttcgtcgcc ctcacttagc aggcgcagca gcgcagcaaa atccgtcagc
     4201 cagggtgaac gatgtaatgt gtgcagcata aacgcctcaa aacctcaaaa taacatcgta
     4261 gttggcgagt tcgcggcgta aggcatctgc ttcgagcggc gtggcttcga caacaaaggg
     4321 tgtctgcgga tctaacccgc gttcgcgcag tgaagccgca caaacccagc actgttcaat
     4381 gtcgtacaga cccaacaatt taaaagtggc gatgtaatca cgcgccagca ctgcatcggg
     4441 cttttgtcct ggcagcagct gaaaaacgcc atcagcaata aagaagacgg ccagatcgtc
     4501 agttaatgcg gaagttgcca gtaaagcatc taaaccttcc cgaccagcag ctgtaccatg
     4561 aggcacagta gaaaaaacaa acgcaattcg tttcatcaga actgtaccac cctgtcacag
     4621 gtcagcgagg cttccgccag cgcgccaagt ccgcttaagg taaatcccgg ctgaaggttt
     4681 gacgaagcca gccccagtct tccggcctcc gtttcatcga cgataccgcg gcgtaacgct
     4741 gccgctacgc agatattcag cgccacacca tgttgcgcat tcaactgttg ccagcagcgc
     4801 acaaggtcaa attcatcgct tgccggtgag gtcaattggt tagcgttata gaccccttcc
     4861 cgataaaaaa agacgctgct taactcatgg ccttctgcaa tcagcgcctg cgcaaactga
     4921 aaagcactac tcgcctgttg cgtaccgtat gctggcccgg tcaccacgat ggcaaaacgc
     4981 attacttatc ttgcccctgg aaatcaccgc tcttgaactg gcggatgtag agatagacag
     5041 tgtgtttgga gatgttcagg cggtcagcaa cctggttgat cgcatcttta atatcgaaga
     5101 tccctttctc gtagagattc agcacgatct gacgattttt ggcgttatta gaaacattgc
     5161 gatcggcatt cacttcttcg atggtgaact ccagcgtttg ggtaaccaga tcttcaacag
     5221 aagaggcaaa gttgacgctt gaaccgacat ccggggtttc tggcggcaca aaggtgctca
     5281 taatctgcga gaagggaaca tcaagattca tattgatgca cagcaaacca attacacgct
     5341 gttcgcggtt acgaatcgcg atagtcaggg acttcattaa tacgccgctt ttggcgcgag
     5401 taaagtagca tttagaaacg ctgctatccg ctcccgtcat atcgtgcagc atacgtagcg
     5461 ccaggtcggt aattggcgaa ccaatcttcc ggcctgtatg ttcaccgtta gcaatgcgaa
     5521 tggctgaaca ttttagatcc tgcaaagagt gcaaaacgat ttcacagtgg gagccaataa
     5581 gcatcgctaa cccgtccacc accgcttcgt aggatttcag aatatcaaaa tcggtctggt
     5641 cgaaaggacg ttgatccagt aaatccaact cactggtttc gttggttaaa agcgacctgg
     5701 acatgaaaaa aaacactcct tttcaggagc ctgtcgttaa cttttcaggg caggctcatt
     5761 aatgatgcgg gtaactaaat taatacagcg gaggttccgc tttccagcac taattatatc
     5821 cggcctgtaa taaaaaaacc gccgcctggt caggcggcgg ttcttaatgc ttatttttta
     5881 gcagaatctg cggctttcgc atcagcttcc ggctttgcat cagccttcgg cgctggtttc
     5941 acatccagca gctctacgtc aaacaccagg gtagaattcg gtgggatccc cggaacaccc
     6001 gctttgccgt aagccagttc tggtggaata accagtttga tcttaccgcc tttcttgatg
     6061 ttcttcagac cttctgtcca acccggaata acaccgtcca gacggaaaga gagcggttca
     6121 ccacgggtgt aagagttgtc gaactcttta ccgtcgatca gcgtaccttt gtagttcacg
     6181 acaacagtat cgctgtcttt cggtgcttcg cctttaccgg cttcgactac ctgataaacc
     6241 agaccagttg aagaggtttt cacacctttc tctttggcaa atttctcgcg gtactcttta
     6301 cctttcgctt cgttatctgc cgcgtctttt tccatcttcg cctgagcaga agacttcacg
     6361 cgagcttcga atgcttgcag tgtctgttcg atctcttggt cggagagttt gctcttatca
     6421 gcaaatgcat cctgaacacc agcgatcagc tgatctttat ccagtttgat gcccagtttt
     6481 tcttgttctt taagagaatt ttccatgtaa cgacccagcg aagcacccag tgcataagct
     6541 gatttctgat cgtcattttt gaacgctgct ttgctgtcag cagttgtagc aggttttgca
     6601 gcttcagcag caaaagtgat cggtgcatgc agggcaacgg ccattgtggt cgccagcagc
     6661 gttactttaa acagtgattt catccatatc tccaggatcg gggcatctca ccccagggtt
     6721 aactattatc agaagggtac tataaagcgt tgtcgaacaa atctacatac agacacgccc
     6781 tattatcatc tattttcaga ctctttttgt ttaaattagt ttcgatgacc gcgaaatgag
     6841 tgctgtctcg ggcagcaaag ttaagtagaa tccgcggcga ccattcgaca aaagaggtga
     6901 atcatgcagg atttatcatt ggaagcacgc ctggcagagc tggagagccg actggctttt
     6961 caggaaatca ccattgaaga actgaacgtc acggtgaccg ctcatgaaat ggagatggcg
     7021 aaactgcgcg atcatctgcg tctgctgacc gagaagctaa aagccagcca gccgtcgaac
     7081 atcgcgtcgc aggctgaaga aacgccaccg ccacattatt gaggcgtaaa aaaagcgggg
     7141 attccccgct tttttgtcac tttttcggta ttagtggcaa ccgcaaccgc cgttaccttt
     7201 accgccgcag cagccttcgc caccgtgttc atgaccgtga tcgtggccat gaccaccgca
     7261 gcaaccgtcg tggtcgtgat cgtggtgatg atcgtgcgca ccgtgaacgt ggccatgagc
     7321 cagttcttct tcagtcgctt cgcgaatcgc cacaacttca acgttgaatt tcaggttctg
     7381 accggccagc atgtggttac catcaaccac gacgtgatcg tcttcaaccg cagtgatttc
     7441 aaccggtacc ggaccctggt cggtttcagc caggaaacgc ataccaacct gcagttcatc
     7501 aacgcccata aatacgtctt taggaacgcg ttgcaccagg ttttcgtcgt actgaccgta
     7561 agcgtcgttc gcgccaacag cgacatcaaa tttgtcgcca acttcgtgac cttccagcgc
     7621 cgtttccagg ccagagatca gggaaccgtg accatgcagg tagtccagcg gcgcactcac
     7681 cggagactca tcaaccaaca caccgtcttc tgtacgtacc tgataggcca ggctgaccac
     7741 caggtctttt gctactttca tgatatctcc tgagcatggg aagaatagtg gcgcagattg
     7801 tagcggaatt ctgcagccgt gtactcacta gcttaaaaaa actcggcgca tatcgctagt
     7861 ccggatgaaa aatcccgatc acttgctcat ctttgcgaac gtgatcgcgg gcttctttgt
     7921 ccgcttctcg catctgatgt ccgcacttaa cacattcaac aatatcaata ttattttcgc
     7981 gccacatcgc cattgaatcc tgcgcctgac aggccgggca ttttgccccc gcaataaaac
     8041 gttttcggat tgccatcttt accctctact caaattcatc ccagccgtcc agctggcgtc
     8101 gttcttgttg catttcacgc tggaaaatct cttccagttc gcgtcgggct tccctggcgc
     8161 gagaaatttg tacggtgtcg gcatgcattg ggatgagctc ccgcaacatc cgcatatcca
     8221 ggcggcgaaa atgcagttgc gcccgctgcg cctgatgtgg atgcatgcct agcgtgacca
     8281 gcgtcttgcg ccccagctct aacgcactgg agaatgtctc acgagaaaac tgcgtcaccc
     8341 ctgcctgtaa taactcatgc gcctccacac gtccgcgcgc tcgcgcaaga atatgtaaat
     8401 gcggaaagtg ctgctggcat atttccacca gtttcatggt gtcttccggc tcattacagg
     8461 taatgacgat agactcagcg gcctctgcac ccgcagaacg taaaagatct acctgcgtgg
     8521 cgtcgccgta ataaactttg tagccatatt tgcgcatcag gttaacggcg ctgatatccc
     8581 gctccagcac agtaatgcgc attttatttg ccatcagcaa acgaccaatc acctgaccaa
     8641 aacgcccgaa gcccacgaca atgacctgcg gtttatcatc gttgacccac ggtttttcgt
     8701 cttcttcttc cggtccgtta aactggcggg atagccattt atccaccagc ttcatcagca
     8761 acggcgtggt catcatggaa agcgtcaccg tcaccagcaa cagcgccatc tggtcgccct
     8821 gaaacaaccg ttgcgaagaa gcggtagaaa agagcacaaa ggcaaactca ccgccctgac
     8881 tcaacacgcc agcaaactgc atccgctctg agctacgcac gccatataat cgcgccagca
     8941 gatacagcac gagaattttc accgtcacca gcacaaccac gcttatcact acccacaaca
     9001 gatgggtata aagcaccccg agattgagcg acatgccgac agagataaag aacaaaccga
     9061 gcagcaagcc tttgaaggga tcgatagctg tttccagttc gtggcgatat tcgctttctg
     9121 ccagcaacac gcccgcaata aacgtaccga gcgccatcga cagccccagc gcatccataa
     9181 acaatgcgga acccaacacc agcagcagcg tcgcggcggt gaacacttcc cgcacgccag
     9241 aagctgcaat aaaacggaat accggacgca gtaaatagcg cccgccaatc agcatgccga
     9301 caaacgccag caccttcatg ccgatcttca tccagtcgaa atgttcgtct gccgaacccg
     9361 ccaacaacgg cactaacgcc agcgccggga tcactgccag atcctgaaac agcagaaccg
     9421 aaaaacctag ctgcccggat tcgctgcgat tcatcccttt ctcacgcatc agttgcaacg
     9481 ccattgcggt tgaggacatc gcaaggccaa taccaccgac caccgccgcc tgccaggcga
     9541 aatccgtcag catcaataat cccgccagca acgccgcgct taacagcacc tgtgccgcac
     9601 ctacgccaaa aatcgaacgc cgcaattgcc aaagcttgga gggattcaac tccaggccga
     9661 tgataaacat caggaacacc acgccgagtt ccgaaaagtg gaggatctca tcgacgtcgc
     9721 taataaaacc cagcccccac gggccaattg caatccctgc cagcaaatat cccaacacag
     9781 cgccaatacc cagccgcgat gccagcggca ccgcagccac cgccgcgaag agaaacaaca
     9841 ctcctgcgag taaaaaatcg gaaccttcca tcagcggcct cctggagaca gcggatttgc
     9901 cagccagtca ccgtaggctc tggcgtgact cgccagctcc tgtgcgcttt gccgtctcgc
     9961 ccagtaaata atgatgggac tcaaccaatg catccggcac atgcccgccg ctagttcaaa
    10021 ggggcgcaga acatcgctca tcgggtagcg attcaaggcg tcataacggt aagcactttc
    10081 cggctcgccg gtggtaatca cgctacgcca gtactttccc gccagttggt ttcctcccgg
    10141 cccgctggca aaaccacggc ttaatacccg gtccagccac tctttcagta gcgccgggca
    10201 gctataggta taaagaggat gctgaaagac aatcacctcg tgctcgcgca gtaatgcctg
    10261 ctcacggggg atatcaataa aaaaatcggg atagtgcgcg taaaggtcgt gcacggtaac
    10321 attgctgagc tgcgtggccg gtttaagcag tacccggttt gccaccgagt cctgagattc
    10381 cggatgggca tacagcagca aaactttcgc tggctgagac atcatccccc tcccggtatg
    10441 gtttttgtgt atagtcgctg ttttgggcta ccattgcgcc cggtgcggca gctcgcccat
    10501 acattacatt atcataatga taagttaaca tagtctgaac atacggcgcc ttatgattgt
    10561 tttttcctcg ttacaaattc gtcgcggcgt gcgcgtcctg ctggataatg ccaccgccac
    10621 catcaacccc gggcagaaag tcggcctggt gggtaaaaac ggctgtggta aatcgaccct
    10681 gctggcattg ctgaaaaatg aaatcagcgc cgacggcggc agctacacct ttccgggaag
    10741 ctggcaactg gcgtgggtga atcaggaaac gccggcgtta ccgcaagcgg cgctggaata
    10801 tgtcattgac ggcgaccgtg aatatcgtca actggaagcg cagctacacg acgccaacga
    10861 acgtaacgac gggcacgcca tcgccactat tcatggcaag ctggatgcta tcgacgcatg
    10921 gagtattcgc tcccgtgccg ccagcctgct gcacggcctc ggtttcagca atgaacaact
    10981 ggagcgcccg gtaagtgatt tctccggtgg ctggcgtatg cgtcttaacc ttgcccaggc
    11041 gctgatttgc cgttcagact tgctgctgct cgatgaaccg actaaccacc tcgatctcga
    11101 tgccgttatc tggctggaaa aatggctgaa gagctatcag ggcacgctga tcctgatctc
    11161 tcacgaccgc gacttcctcg atccgatcgt tgataaaatt attcatatcg aacaacaaag
    11221 catgttcgag tacaccggca actacagttc gtttgaagta cagcgcgcca cccgtctggc
    11281 gcagcaacaa gcgatgtatg aaagccagca ggaacgcgtg gcgcatctgc aaagttatat
    11341 cgaccgtttc cgtgccaaag ccaccaaagc gaagcaggcc cagagccgta ttaagatgct
    11401 ggagcgtatg gagctgattg ccccggcgca cgttgacaac ccgtttcgtt ttagcttccg
    11461 cgcgccggaa agcctgccta atccgttatt aaagatggaa aaagtcagcg caggctatgg
    11521 tgatcgcatt attctcgact cgattaaact gaatctggtc cccggctcgc gcattggtct
    11581 gctaggccgc aacggcgcgg gtaaatcgac attaatcaaa ctgttagccg gcgagcttgc
    11641 gccagtcagc ggtgaaattg gtctggcgaa agggatcaag ctcggctact tcgcccagca
    11701 tcaactggaa tacctgcgcg ccgacgaatc gcctattcaa catctggcac gtttagcgcc
    11761 gcaggaactg gagcaaaaac tgcgtgacta cctcggcggc tttggtttcc agggcgataa
    11821 agtgacggaa gaaacgcgcc gcttctccgg tggggaaaaa gcccgcctgg tgctggcatt
    11881 aattgtctgg cagcgtccga atctgctgct gctcgacgaa ccgactaacc accttgacct
    11941 cgacatgcgt caggcactga ctgaagcatt aatcgagttc gaaggcgcgc tggttgtcgt
    12001 ttcgcacgac cgtcatttgc tgcgttccac cactgacgat ctctacctgg ttcacgatcg
    12061 taaagtcgaa ccgttcgacg gcgatctgga agattatcaa cagtggttga gcgacgtaca
    12121 aaagcaggaa aaccagaccg acgaagcgcc aaaagagaac gcaaacagcg cccaggcacg
    12181 taaagatcag aagcgtcggg aagcggagct gcgtgcgcaa acccaaccgc tgcgtaaaga
    12241 gatcgcccgt ctggaaaaag agatggaaaa gctgaacgca cagctggcgc aggcggaaga
    12301 gaaactcggc gacagcgaac tgtacgatca aagccgtaaa gcggagttga ccgcctgcct
    12361 gcaacagcaa gccagcgcca aatccggcct ggaagagtgc gaaatggcgt ggctggaagc
    12421 ccaggagcag cttgagcaga tgctgctgga aggccaaagc aactgatggc gcagataacg
    12481 acgaccgatg ccaatgaatt cagcagcagt gctgaattca cccctatgcg cggctttagc
    12541 aattgtcatc tacaaaccat gctgccgcgt ctgtttcgtc gcaaggtgaa attcaccccg
    12601 cactggcagc gactggagtt gcccgacggc gattttgtcg atctcgcgtg gagtgaagat
    12661 cctgcacagg cgcggcataa accgcgttta gtcgtgtttc acgggctcga aggcagtctc
    12721 aatagccctt acgcccacgg tctggttgag gcggcgcaaa agcgcggctg gctgggcgtg
    12781 gtgatgcatt ttcgcggatg cagcggtgaa ccaaaccgta tgcaccgcat ttaccattcg
    12841 ggtgaaaccg aagacgccag ctggttttta cgctggctgc agcgcgaatt tggacatgcg
    12901 ccaacggctg ccgtcggcta ttcgctcggc ggtaatatgc tcgcctgttt gctggcaaaa
    12961 gaaggtaata acctcccgat tgatgcagcg gtaattgtct ccgcgccatt tatgctggaa
    13021 gcctgtagtt atcatatgga aaagggcttt tcccgcgttt atcagcgtta cttgctgaac
    13081 ctgttaaaag ccaatgccgc gcgcaagctg gcagcctacc ccggaacact gccgattaat
    13141 ctcgcgcagt taaaatcggt acgtcgcatc cgtgaatttg acgatctaat caccgccaga
    13201 attcacggct acgctgacgc tatcgactat tatcgtcagt gtagcgccat gccgatgctc
    13261 aatcggatcg ccaaaccgac gctgattatt cacgccaaag acgatccgtt tatggatcat
    13321 caggtgatcc cgaaaccgga aagtctcccc ccgcaggtgg agtatcaact gactgaacat
    13381 ggcggtcatg ttggctttat tggcggtaca ttacttcatc cgcaaatgtg gctggagtca
    13441 cgcattcctg actggttaac aacgtatctg gaggcgaaat catgttgatt ccgtggcaag
    13501 acctctcccc cgaaacgctg gaaaatttaa ttgaaagctt tgtgttacgt gaaggcaccg
    13561 attatggtga acatgagcgt acacttgaac agaaagtcgc cgacgtcaaa cgccagctac
    13621 agtgcggaga agcggtgctg gtatggtcgg agctgcacga aacggtcaat atcatgccgc
    13681 gcagtcagtt tcgcgaataa cctgcaaccg tcggcggacg agataataat gatagctaag
    13741 tcatggagtt actatgtctg ccaaacatcc ggtcattgcg gtaacaggat ccagcggcgc
    13801 ggggaccacc accaccagcc tcgcgtttcg taaaatattc gcgcagttaa atctgcatgc
    13861 agccgaggtg gaaggcgaca gttttcaccg ttacacccgc ccggaaatgg acatggcgat
    13921 ccgcaaagca cgcgacgccg ggcggcatat cagctacttc ggccccgaag cgaacgactt
    13981 cggcctgctg gaacaaacct tcattgaata tggtcagagc ggcaaaggga aatctcgcaa
    14041 atatctgcat acctacgatg aagccgtacc gtggaatcag gtaccgggga cattcacccc
    14101 ctggcaacct ttaccggaac ccactgatgt actgttttat gaaggtttac acggcggcgt
    14161 agtcacgcca cagcataacg ttgcgcagca tgtggactta ctggtcggcg tggtgcctat
    14221 cgttaacctt gagtggattc aaaaactgat ccgcgacacc agcgagcgcg ggcactcacg
    14281 agaagcagtg atggactcgg tagtgcgttc aatggaagac tatatcaact acatcacacc
    14341 gcagttttcc cgcacccatc ttaacttcca gcgcgttccc accgtcgaca cttcaaaccc
    14401 gttcgcggca aaaggtatcc cgtcgctgga tgaaagcttt gtggtgatcc attttcgtaa
    14461 tctggaaggg atcgatttcc cctggctgct ggcgatgttg caaggctcat tcatttccca
    14521 catcaatacg ttagtggtac cgggcggcaa aatgggtctg gcaatggaat taattatgct
    14581 gccgctggtg caacgattga tggaaggaaa gaaaatcgag taactttgct attacgccgg
    14641 ataaaatact atccggcttc acaatgggat agttaaatca cgcggcaacc acttcatacg
    14701 agtgagtaat attcaccgct ttttccagca tcaacgccac tgaacaatat ttctcggcag
    14761 agagatcaac cgcacgtgca accgctgcgt ctttcaggtc gcgaccggta acgataaaat
    14821 gcagattaat gtgcgtaaac aggcgtggtg cctcttcgcg gcgttcagag gtcaatttta
    14881 cttcacaatc gaccacatcc tgacgccctt tttgcaggat agaaaccaca tcgatggcac
    14941 tgcaaccccc cgccgccatc aacaccattt ccatcggact tggtgcttta tcgcctgagt
    15001 tgccgtccat taaaatctgg tgaccagagg cggattcgcc cagaaaagtt aacccttcga
    15061 cccacttcac tcgcgcttgc atttttgcta ctccactgcg tcaattttcc tgacagagta
    15121 cgcgtactaa ccaaatcgcg caacggaagg cgacctgggt catgctgaag cgagacacca
    15181 ggagacacaa agcgaaagct atgctaaaac aatcaggatg ctacagtaat acattgatgt
    15241 actgcatgta tgcaaaggac gtcacattac cgtgcagtac agttgatagc cccttcccag
    15301 gtagcgggaa gcatatttcg gcaatccaga gacagcggcg ttatctggct ctggagaaag
    15361 cttataacag aggataaccg cgcatggtgc ttggcaaacc gcaaacagac ccgactctcg
    15421 aatggttctt gtctcattgc cacattcata agtacccatc caagagcacg cttattcacc
    15481 agggtgaaaa agcggaaacg ctgtactaca tcgttaaagg ctctgtggca gtgctgatca
    15541 aagacgaaga gggtaaagaa atgatcctct cctatctgaa tcagggtgat tttattggcg
    15601 aactgggcct gtttgaagag ggccaggaac gtagcgcatg ggtacgtgcg aaaaccgcct
    15661 gtgaagtggc tgaaatttcg tacaaaaaat ttcgccaatt gattcaggta aacccggaca
    15721 ttctgatgcg tctgtctgca cagatggcgc gtcgcctgca agtcacttca gagaaagtgg
    15781 gcaacctggc gttcctcgac gtgacgggcc gcattgcaca gactctgctg aacctggcaa
    15841 aacaaccgga tgctatgact cacccggacg gtatgcaaat caaaattacc cgtcaggaaa
    15901 tcggtcagat tgtcggctgt tctcgtgaaa ccgtgggacg cattctgaag atgctggaag
    15961 atcagaatct gatctccgca cacggtaaaa ccatcgtcgt ttacggcact cgttaatccc
    16021 gtcggagtgg cgcgttacct ggtagcgcgc cattttgttt cccccgatgt ggcgcagact
    16081 gatttatcac cccgatatta actatgcact gcgacaaacg ctggtgctat gtttgcccgt
    16141 ggccgttggg ttaatgcttg gcgaattacg attcggcctg ctcttctccc tcgttcctgc
    16201 ctgttgcaat attgcgggcc ttgatacgcc tcataaacgt tttttcaaac gcttaatcat
    16261 tggtgcgtcg ctgtttgcca cctgtagctt gctgacacag gtgctactgg caaaagatgt
    16321 tcccctgccc tttttgctga ccggattaac gctggtactt ggcgtcactg ctgaactggg
    16381 gccattgcac gcaaaattgc ttcccgcatc gctgctcgcc gccattttta ccctcagtct
    16441 ggcaggatac atgccggtct gggaaccgtt gctcatctat gcgttgggca ctctctggta
    16501 cggattgttt aactggtttt ggttctggat ctggcgcgaa caaccgctgc gcgagtcact
    16561 aagtctgctg taccgcgaac tggcagatta ttgtgaagcc aaatacagcc tgcttaccca
    16621 gcacaccgac cctgaaaaag cgctgccgcc gctgcttgtg cgccagcaaa aagcggtcga
    16681 tctaattacc cagtgctatc agcaaatgca tatgctttcc gcgcaaaata ataccgatta
    16741 caagcggatg ctgcgtattt tccaggaggc gctggatttg caggaacata tttcggtcag
    16801 tttgcatcag ccggaagagg tgcaaaaact ggtcgagcgt agccatgcgg aagaagttat
    16861 ccgctggaat gcgcaaaccg tcgccgctcg cctgcgcgtg ctggccgatg acattcttta
    16921 tcatcgcctg ccgacgcgtt ttacgatgga aaagcaaatt ggcgcactgg aaaaaatcgc
    16981 ccgccagcat ccggataatc cggttgggca attctgctac tggcatttca gccgcatcgc
    17041 ccgcgtgctg cgcacccaaa aaccgctcta tgcgcgtgac ttactggccg ataaacagcg
    17101 gcgaatgccg ttacttccgg cgctgaaaag ttatctgtca ctaaagtctc cggcgctacg
    17161 caatgccgga cgactcagtg tgatgttaag cgttgccagc ctgatgggca ccgcactgca
    17221 tctgccgaag tcgtactgga tcctgatgac ggtattgctg gtgacgcaaa atggctatgg
    17281 cgcaacccgt ctgaggattg tgaatcgctc cgtgggaacc gtggtcgggt taatcattgc
    17341 gggcgtggcg ctgcacttta aaattcccga aggttacacc ctgacgttga tgctgattac
    17401 caccctcgcc agctacctga tattgcgcaa aaactacggc tgggcgacgg tcggttttac
    17461 tattaccgca gtgtataccc tgcaactgtt gtggctgaac ggtgagcagt acatccttcc
    17521 gcgtcttatc gataccatta ttggttgttt aattgctttc ggcggtactg tctggctgtg
    17581 gccgcagtgg cagagcgggt tattgcgtaa gaacgcccat gacgctttag aagcctatca
    17641 ggacgcgatt cgcttgattc tgagcgagga cccgcaacct acgccactgg cctggcagcg
    17701 aatgcgggtg aatcaggcgc ataacactct gtataactca ttgaatcagg cgatgcagga
    17761 accggcgttt aacagccatt atctggcaga tatgaaactg tgggtaacgc acagccagtt
    17821 tattgttgag catattaatg ccatgaccac gctggcgcgg gaacaccggg cattgccacc
    17881 ggaactggca caagagtatt tacagtcttg tgaaatcgcc attcagcgtt gtcagcagcg
    17941 actggagtat gacgaaccgg ggagttctgg cgatgccaat atcatggatg cgccggagat
    18001 gcagccgcac gaaggcgcgg caggtacgct ggagcagcat ttacaacggg ttattggtca
    18061 tctgaacacc atgcacacca tttcgtcgat ggcatggcgg cagcgaccgc atcacgggat
    18121 ttggctgagt cgcaagttgc gggattcgaa ggcgtaatac aggctaaacc atcggatgag
    18181 gcgttcacgc cgcatccgac atcttttgcc tgacgcgtct tatttacgcc ccaatcacct
    18241 tcgccaccgc gtgggcgaaa cgatgcatcc cttcatcgat atccgcatct tccaccacca
    18301 gcgacggcgc aaaacgcatc acatcaggcc cggcattcag caccattacg ccttcctctg
    18361 caccggcata caggaaatca cgcgcccgac ctttgtactg tggtttcagc tccgcgccaa
    18421 ttaacagccc cataccgcga atatcgctaa acacatcgta ctgctgatcg atcttctgca
    18481 aatggtcaac aaaacgctgg cgtttcgcct gaatgccttc cagcacttca ggggtattga
    18541 tgatatcaaa cgccgcgccc gctactgcac aggccagagg attaccgccg taggtggaac
    18601 cgtgagaacc aggatgaaat gcagaagcaa tttccgccgt ggtcagcatg gcgctaatcg
    18661 ggaagccgcc gcctaacgct ttcgcagagg tcagaatatc cggcgtcaca ccgtagtgca
    18721 tgtaagcaaa caaatcgcca gtccgcccca tcccgcactg cacttcatca aacaccaata
    18781 atgcctgatg ttgatcgcac agctcgcgca agccctgcaa aaactctggc gtcgctgccg
    18841 tcacaccgcc ctcgccctgg atcggctcaa ccaccaccgc acaggtatgg tcatccatca
    18901 ccgctttcac tgcgtggaga tcgttaaaag gcacgtggat gatgtctgcc ggttttggcc
    18961 caaagccgtc ggaatatttt ggctgcccac ccaccgaaac ggtaaacagc gagcgaccat
    19021 gaaaagcgtt atggaaggca ataattttgg ttttgaacgg gctatgacgc acacaggcgt
    19081 aatggcgtgc cagtttaaag gcggtttcgt tagcttccgt gccggagttc ataaacacca
    19141 cgcgttcggc aaaagttgcc tcaatcagtt tacggccaag acgcagcgcc ggctcattgg
    19201 tgaaaacgtt actaatatgc cacagagttt cgccctgggt ttttaacgcg ttcaccagcg
    19261 caggatggca atggcccaac gccgtaactg caatgccacc cgcgaaatcg acatactcct
    19321 tgccctgttg atcccagatt cggctgccct gaccttttac cggaataaac tctgccggag
    19381 cataaatcgg cagaatcact tcatcgaaag tcgcgcgtgt aattgctgtt tgttcaattg
    19441 ccatctcatg atcaccctgt tacgcataaa taaatatgaa attataatca caaaatatgc
    19501 ataaaaaatc actgaatggc aatcagaaat cagcgatgca gaaaattagc cagcagttga
    19561 tgtccttgtt cgctgagaat actttctgga tggaattgca caccttccag atcccactgg
    19621 cgatggcgaa tccccataat ctcgcgggtt tcgctccagg ccgtcacttc aaagcactcc
    19681 ggtaatgagt caggttccac caccagcgaa tggtagcgtg tcacggtgag tggatttgcc
    19741 agccccttaa atacgcccac accattatgt gtaatcggcg aggttttgcc gtgcatgacc
    19801 tttgcggcgc gcacaacttt gccgccaaat gcctgcgcca ttgcctgatg accgaggcag
    19861 acgccaagaa tcggcaagcg tccggcatag tgacgaataa cgtcaaggga gatcccggct
    19921 tcatctggcg tacaggggcc gggtgagatg acaatttttt gtggtttaag ggcgtcgata
    19981 tccgccaacg tcaacgcatc gttgcgctta accaacacat ccgcccccag ttcacaaaag
    20041 tactggtaga ggttccaggt aaaagaatcg tagttatcaa taagcaggat catggcggct
    20101 ccggtacgaa agaaccgcgc tattttactc agattccccg gcttcgctta ccactttgct
    20161 aaatatcatc tgcagcgcgg tgagatcccc cattgcccca ttctgatttg cctgattcca
    20221 ggcctctttt tcaataccct gccagctcag ttgatatccg gcatgaatcg ccagttgctc
    20281 gaagaagatc cgctgtgcca aaccacttcc cacccggaag ggatgcagca cgttgatttc
    20341 acaatagtaa tgcgccagcc gctcgacgaa cttcgctttc tccaggccaa ccagataacc
    20401 ttcttcctcc agatcctgca tcagggcatt gccctctttt tcgatataag caaagtggca
    20461 gaacggcgta tcgccctgat aaatatcgac ttcgcgcagt tgccccgccc agtcgaaaat
    20521 atcctgatac aactggcgat gaagagtacg caaatgcggt aaaccccgaa ccaacgggcc
    20581 aagttcaatg gtcgcagcac gcagcgccgt catttcgtaa gcggcctgtt ccagccgctg
    20641 ctgctggcgg atgttcagcc ggttacgcat gatatcaagg cctggataaa gatacggatc
    20701 gcgcccttcg ccgaatttat cgctcatagt gcctcctcag cgcttcaaga cgcgctaaag
    20761 cctctgcggc agtaagagtg actaaaggca tctcgacgcc ttcaagacgg cgactggcct
    20821 ggaaattacg attacgctga agctcccaga gacgggactt ttgcttatcg gtgagtttct
    20881 tcacttgatg cctccctgaa cgtgtccatt tgccacaagt ataagcggca aatccgggtt
    20941 acgccgggag agcagaagcg cgggcaagat tgcccgcgcg agaaatcatt acggcaggac
    21001 tttagcggaa aggataacta ccggttttga cggcacattc tggtacggac caacgtcatg
    21061 agtcggcacc tgggaaatct tatcggcaac gtccatgcct ttcaccactt taccaaatac
    21121 cgcgtaaccg aaatcacgct gaccgtggtc aaggaaggcg ttatcggcaa cgttgataaa
    21181 gaactggctg gtggcgctgt ctttgtcagc ggtacgcgcc atcgcgatgg tgccacgcgt
    21241 gttgcgcagg ccgttatcag cttcattttt gattggcggg tttggttttt tctgctgcat
    21301 ctgctcggtg aaaccgccgc cctgaatcat aaagccagga atgacgcggt gaaaggtcat
    21361 gttgttataa aaaccactgt tcacataatc gacaaagttt tgcacagaca ctggcgcttt
    21421 ttgtttatcc agctccagtt cgatgttacc agctgaggtt gtcaacaata cgtgcgggtc
    21481 ccctttcgct gccattgctg cgggagaaag agcagaaaga gcgaaaacag cagccatcgc
    21541 cgccagggtc gatttgaaca tgagatttcc ttaacaaaca gagcagaaaa aaagcaagtg
    21601 aagagattct aaatagcctt caagaacaag gccatccctt tacctaattt tacgtatctg
    21661 aaatatatgt aacctgagga taacttttta ttccgtgatc aaaatcacct cttaaaatgc
    21721 aatttagcaa ccgattgcaa taaaacattt aatcagatca caaaatcgcc taaaatcgcc
    21781 cgctcgcggc atagcccgct atgccatttt atttttgaac gcaggccaat catgactaac
    21841 agcaatcgca tcaagctcac atggattagc tttctttcct acgcactgac cggtgcgttg
    21901 gttattgtca ccgggatggt gatgggaaat atcgccgatt atttcaatct gcctgtttcc
    21961 agcatgagta acaccttcac cttcctcaat gccggcattt taatctctat cttcctcaac
    22021 gcctggctga tggaaatcgt cccgttgaaa acgcagttac gttttggctt tctcctgatg
    22081 gtgctggcgg ttgccggttt gatgttcagc cacagcctgg cgctgttctc ggcggcgatg
    22141 ttcattctcg gggtggtcag cggcatcacc atgtcgattg gtacattcct ggtaacacaa
    22201 atgtatgaag gacgtcagcg cggttcacgc ctgttattta ccgactcctt cttcagtatg
    22261 gccgggatga ttttcccaat gatcgccgcg tttctgctgg cacgtagcat tgagtggtac
    22321 tgggtttatg cctgcatcgg gctggtgtat gttgctattt ttattctgac cttcggctgt
    22381 gaattcccgg cactgggcaa acatgcgcca aaaacagatg ctccggtaga gaaagaaaag
    22441 tgggggatcg gcgtactgtt tctctctgtt gcggcgctgt gctacatcct cggtcagtta
    22501 ggttttatct cctgggtgcc ggaatatgcc aaaggcctgg gcatgagcct gaacgacgca
    22561 ggcacgctgg taagtaactt ctggatgtcg tacatggtcg gcatgtgggc gttcagcttt
    22621 attcttcgct tctttgattt gcaacgtatc ctgatcgtac tggctggtct ggctgcgatt
    22681 ctgatgtacg tctttaacac cggaacaccg gcatatatgg cgtggtcaat tctcgccctg
    22741 ggcttcttct ccagcgcgat ctataccacc atcatcaccc tgggttcaca gcagaccaaa
    22801 gtaccgtcgc caaaactggt taactttgtc ctgacctgcg ggaccatcgg tactatgttg
    22861 acctttgtgg ttaccggccc gattgttgaa catagtggtc cgcaggcggc gctgctgacg
    22921 gcaaacggtc tgtacgctgt cgtctttgtg atgtgcttcc tgttaggttt cgtcagccgt
    22981 caccgtcagc ataacaccct gacctctcat taattgttca tgccgggcgg cactatcgtc
    23041 gtccggcctt ttcctctctt actctgctac gtacatctat ttctataaat ccgttcaatt
    23101 tgtctgtttt ttgcacaaac atgaaatgtc agacaattcc gtgacttaag aaaatttata
    23161 caaatcagca atatacccct taaggggtat ataaaggtga atttgattta catcaataag
    23221 cggggttgct gaatcgttaa ggtaggcggt aatagaaaag aaatcgaggc aaaaatgagc
    23281 aaagtcagac tcgcaattat cggtaacggt atggtcggcc atcgctttat cgaagatctt
    23341 cttgataaat ctgatgcggc caactttgat attaccgttt tctgtgaaga accgcgcatc
    23401 gcttatgacc gcgtacacct ctcgtcttac ttctctcacc acaccgccga agagctgtcg
    23461 ctggtgcgcg aaggcttcta cgagaaacac ggcatcaaag ttctggtcgg cgaacgcgct
    23521 atcaccatca accgtcagga gaaggtgatt cactccagcg ccggacgtac cgttttttat
    23581 gacaagctga tcatggcgac cggttcctac ccgtggatcc cgccaattaa aggttctgat
    23641 actcaggact gctttgtcta tcgcaccatt gaagacctca acgccattga atcctgcgct
    23701 cgtcgcagca agcgcggtgc cgttgttggt ggcggcctgt taggtctgga agctgctggc
    23761 gcgctgaaaa acttaggcat tgaaacccac gttatcgaat ttgcccctat gctgatggcg
    23821 gaacagcttg atcagatggg cggcgagcag ctgcgtcgca aaatcgaaag tatgggcgtg
    23881 cgcgttcaca ccagcaaaaa cactcttgag attgtgcagg aaggtgttga agcgcgtaaa
    23941 accatgcgtt ttgccgacgg cagcgaactg gaagtcgact ttatcgtctt ctctaccggt
    24001 atccgtccgc gcgataagct ggcaacccag tgtggtctgg acgttgctcc gcgtgggggt
    24061 atcgtcataa atgattcctg ccagacttcc gatccggata tctacgccat cggtgaatgc
    24121 gcaagctgga acaaccgtgt atttggtctg gtagcaccag gctacaaaat ggcgcaggtc
    24181 gccgttgacc atattcttgg tagcgaaaac gcctttgaag gtgcagacct tagcgccaag
    24241 ctgaaactgc tgggcgtaga cgtaggcggt attggtgatg cgcacggtcg cacacctggc
    24301 gcacgtagct acgtttacct cgacgaaagc aaagagatct acaaacgcct tattgtcagc
    24361 gaagacaaca aaaccctgct cggtgcggta ctggtgggcg ataccagcga ctacggcaac
    24421 ctgctgcaac tggtgctgaa tgctatcgag ctgccggaaa acccggattc tctgattctg
    24481 ccggcgcact cgggtagcgg taagccgtct atcggtgttg ataaactgcc ggacagcgcg
    24541 caaatctgct cctgcttcga cgtcaccaaa ggtgatctga ttgctgccat caacaaaggc
    24601 tgccacaccg ttgcggcgct gaaagctgaa accaaagcgg gtactggctg cggtggctgt
    24661 atcccgctgg tcactcaggt actgaacgcg gaactggcga aacagggcat cgaagttaac
    24721 aacaacctgt gcgaacactt tgcttattcg cgtcaggaac tgttccattt gatccgcgtt
    24781 gaaggcatta aaaccttcga agaactgctg gcgaaacacg gcaaaggcta cggttgtgaa
    24841 gtttgtaaac cgaccgtcgg ttcgctgctg gcctcctgct ggaacgaata cattctgaaa
    24901 ccggaacata ctccgctgca ggattctaac gataacttcc tcgctaacat ccagaaagac
    24961 ggcacctact cggtgatccc gcgttctccg ggcggtgaaa tcaccccgga agggctgatg
    25021 gcagtaggtc gtatcgcgcg tgaatttaat ctctacacca aaatcaccgg ctcccagcgc
    25081 ctggcgatgt ttggcgcaca gaaagacgat ctgccggaga tctggcgtca gctgattgaa
    25141 gccggcttcg aaaccggtca tgcctatgcg aaagcactgc gtatggcgaa aacctgcgtg
    25201 ggtagcacct ggtgccgcta cggcgtgggc gacagcgtcg gcctcggcgt ggaactggaa
    25261 aaccgctaca aaggtatccg gactccgcac aaaatgaagt tcggtgtctc cggctgtacc
    25321 cgtgaatgtt cagaagctca gggtaaagac gtgggtatta tcgccaccga aaaaggctgg
    25381 aacctgtatg tttgcggtaa cggcggcatg aaaccgcgtc atgcgaatct gttggcagcg
    25441 gatatcgatc gcgaaacgct gatcaaatat ctcgaccgct tcatgatgtt ctacatccgt
    25501 accgccgaca aactgacgcg taccgcgccg tggttagaaa acctcgaagg tggcatcgat
    25561 tacctgaaag cggtgatcat tgacgacaaa ctggggctga acgcacatct ggaagaagag
    25621 atggcgcgcc tgcgtgaagc ggtagtgtgt gagtggactg aaacggtcaa taccccgtct
    25681 gcgcagactc gcttcaaaca cttcatcaac agcgataagc gtgacccgaa cgtgcagatg
    25741 gtgccagagc gcgaacagca ccgtccggca acgccgtatg aacgtatccc ggtaactctg
    25801 gtggaggaca acgcatgagc cagtggaaag acatctgcaa aatcgatgac atcctgcctg
    25861 aaaccggcgt ctgcgcgctg ttaggtgacg aacaagtagc aattttccgc ccgtatcaca
    25921 gcgatcaggt gtttgcgatc agcaacatcg atccgttctt cgagtccagc gtgctgtcac
    25981 gcgggctgat tgcggaacac cagggcgagc tgtgggtcgc cagcccgctg aaaaaacagc
    26041 gttttcgctt aagtgatggc ttgtgcatgg aagacgaaca gttttccgtc aaacattacg
    26101 aagcacgagt gaaagacggc gtggtgcagc tgcgcggtta atgttttaac gggaggcgca
    26161 atgcctcccc tttttgcatg gtcctgtaat gaccttcggt atattgcagg acatttttta
    26221 aactttttgt tttatttttt tgtttttatt ttttaaagga taatcaaatg tttacagaca
    26281 ctattaataa gtgtgcggct aacgctgcgc gcattgcacg cctgtcggca aataacccgc
    26341 tcggcttttg ggtcagctcc gccatggcgg gcgcgtatgt gggtcttggg atcatcctga
    26401 ttttcacgct cggtaatttg ctcgatccgt ccatacgtcc tctggtgatg ggcgcgacct
    26461 ttggtatcgc cttaacgctg gtgattatcg ccggttctga actgttcacc ggacacacca
    26521 tgttcctcac ctttggggtg aaagcgggca ccatcagcca cgggcaaatg tgggcaatcc
    26581 tgccgcaaac ctggctgggt aatctggtcg gttccgtctt cgttgccatg ctctatagct
    26641 ggggcggcgg tagcctgctg ccggtagata ccagcatcgt tcactccgtc gcgctggcaa
    26701 aaaccactgc accggcaatg gtactgttct tcaaaggtgc attgtgtaac tggctggttt
    26761 gcctggctat ctggatggcg ctgcgcactg aaggggcggc gaaatttatc gctatctggt
    26821 ggtgtctgct ggcatttatc gcgtccggct acgagcactc catcgctaac atgacgctgt
    26881 tcgccctctc ctggttcggc aaccacagcg aagcctacac gctggcgggt attggtcata
    26941 acctgctgtg ggtgacgctg ggtaatactt tatcaggtgc cgtattcatg ggattgggtt
    27001 attggtatgc tacgccgaaa gcgaatcgtc cggttgcgga caaatttaat caaactgaaa
    27061 cagctgccgg ttaattacta aggggttttt acgtggatca tttgcctata ttttgccaat
    27121 tacgcgatcg cgactgtctg attgtcggcg gtggtgatgt cgcggaacgc aaagcaaggt
    27181 tactgttaga cgcaggcgct cgcttaacgg tgaatgcatt agcgtttatt ccacagttca
    27241 ccgcatgggc agatgcaggc atgttaaccc tcgtcgaagg gccatttgat gaaagccttc
    27301 tcgacacctg ctggctggcg attgctgcga cggatgatga cgcgcttaac cagcgcgtca
    27361 gtgaagccgc tgaagctcgt cgcatcttct gtaacgtggt cgatgcgccg aaagccgcca
    27421 gctttattat gccgtcaatt attgaccgct caccgctaat ggtcgcggtc tcctctggcg
    27481 gcacctctcc ggttctggcg cgcctgttgc gcgaaaaact cgaatcactg ctgccgctgc
    27541 atctgggcca ggtagcgaaa tacgccgggc aattacgcgg gcgagtgaaa caacagttcg
    27601 ccacgatggg tgagcgtcgc cgtttctggg agaaattgtt cgttaacgac cgcctggcgc
    27661 agtcgctggc aaacaacgat cagaaagcca ttactgaaac caccgaacag ttaatcaacg
    27721 aaccgctcga ccatcgcggt gaagtggtgc tggttggtgc aggtccgggc gatgccgggc
    27781 tgctgacact gaaaggtctg caacaaattc agcaggcaga tgtggtggtc tacgaccgtc
    27841 tggtttctga cgatattatg aatctggtac gccgcgatgc tgatcgcgtt ttcgtcggca
    27901 aacgcgcggg ataccactgc gtaccgcagg aagagataaa ccagatcctg ctacgggaag
    27961 cgcaaaaagg caaacgcgtg gtgcggctga aaggcggcga tccgtttatt tttggccgtg
    28021 gtggcgaaga gctggaaaca ctgtgcaacg cgggtattcc gttctcggtg gttccgggta
    28081 ttaccgcagc ttctggttgc tctgcctatt cgggtattcc gctcacgcat cgcgattatg
    28141 cccagagcgt acgcttaatt accggacact taaaaaccgg tggcgaactg gactgggaaa
    28201 acctggcggc agaaaaacag acgctggtgt tctatatggg gttgaatcag gccgcgacta
    28261 ttcagcaaaa gctgattgaa cacggtatgc ctggcgaaat gccggtggca attgtcgaaa
    28321 acggaacagc agtcacgcag cgcgtgattg acggtacgct cacacaactg ggcgaactgg
    28381 cgcagcaaat gaacagtcca tcgctaatta ttattggtca ggttgttggc ctgcgcgata
    28441 aattgaactg gttctccaac cattaattta accaaggcca gagaatt
//
LOCUS       DAZGQL010000071        27800 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.071, whole genome shotgun sequence.
ACCESSION   DAZGQL010000071 DAZGQL010000000
VERSION     DAZGQL010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27800
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.071"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            73..336
                     /gene="yhcN"
                     /locus_tag="ACHKIR_003334"
     CDS             73..336
                     /gene="yhcN"
                     /locus_tag="ACHKIR_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HFO9991021.1"
                     /translation="MKIKTTVTALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(391..663)
                     /gene="yhcO"
                     /locus_tag="ACHKIR_003335"
     CDS             complement(391..663)
                     /gene="yhcO"
                     /locus_tag="ACHKIR_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HFO9991022.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQAFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(755..2722)
                     /gene="aaeB"
                     /locus_tag="ACHKIR_003336"
     CDS             complement(755..2722)
                     /gene="aaeB"
                     /locus_tag="ACHKIR_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HFO9991023.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            complement(2728..3660)
                     /gene="aaeA"
                     /locus_tag="ACHKIR_003337"
     CDS             complement(2728..3660)
                     /gene="aaeA"
                     /locus_tag="ACHKIR_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HFO9991024.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQVLFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            complement(3668..3871)
                     /gene="aaeX"
                     /locus_tag="ACHKIR_003338"
     CDS             complement(3668..3871)
                     /gene="aaeX"
                     /locus_tag="ACHKIR_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump operon protein
                     AaeX"
                     /protein_id="HFO9991025.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            4054..4983
                     /gene="aaeR"
                     /locus_tag="ACHKIR_003339"
     CDS             4054..4983
                     /gene="aaeR"
                     /locus_tag="ACHKIR_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HFO9991026.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            complement(5111..6556)
                     /gene="tldD"
                     /locus_tag="ACHKIR_003340"
     CDS             complement(5111..6556)
                     /gene="tldD"
                     /locus_tag="ACHKIR_003340"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HFO9991027.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(6712..10512)
                     /gene="yhdP"
                     /locus_tag="ACHKIR_003341"
     CDS             complement(6712..10512)
                     /gene="yhdP"
                     /locus_tag="ACHKIR_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HFO9991028.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGGNDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGQVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGDV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLKNV
                     SSHLPSPLAKPAGEPLPVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTH
                     GDSTNSSPFPTTERISFRGWPDAQIRCAECWFWGQKFGRIDSDITISGNTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            complement(10580..12049)
                     /gene="rng"
                     /locus_tag="ACHKIR_003342"
     CDS             complement(10580..12049)
                     /gene="rng"
                     /locus_tag="ACHKIR_003342"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HFO9991029.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            complement(12039..12632)
                     /gene="yhdE"
                     /locus_tag="ACHKIR_003343"
     CDS             complement(12039..12632)
                     /gene="yhdE"
                     /locus_tag="ACHKIR_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HFO9991030.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            complement(12641..13129)
                     /gene="mreD"
                     /locus_tag="ACHKIR_003344"
     CDS             complement(12641..13129)
                     /gene="mreD"
                     /locus_tag="ACHKIR_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFO9991031.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            complement(13129..14232)
                     /gene="mreC"
                     /locus_tag="ACHKIR_003345"
     CDS             complement(13129..14232)
                     /gene="mreC"
                     /locus_tag="ACHKIR_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFO9991032.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGITQPTPQQPATGNAVTA
                     PAAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            complement(14298..15341)
                     /gene="mreB"
                     /locus_tag="ACHKIR_003346"
     CDS             complement(14298..15341)
                     /gene="mreB"
                     /locus_tag="ACHKIR_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HFO9991033.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            complement(15646..17586)
                     /gene="csrD"
                     /locus_tag="ACHKIR_003347"
     CDS             complement(15646..17586)
                     /gene="csrD"
                     /locus_tag="ACHKIR_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HFO9991034.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSTLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGTND
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLCEIQNAREQHSRLDT
                     LIRSYAAQDMKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFSLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIASQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGRVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLLISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRRRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            17738..18712
                     /gene="yhdH"
                     /locus_tag="ACHKIR_003348"
     CDS             17738..18712
                     /gene="yhdH"
                     /locus_tag="ACHKIR_003348"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HFO9991035.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEVVVTGASGGVG
                     STAVTLLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPARRA
                     QAWQRLVADLPESFYTQAAKEISLAEAPKFAEAIINNQIQGRTLVKVN"
     gene            18898..19285
                     /locus_tag="ACHKIR_003349"
                     /pseudo
     CDS             18898..19285
                     /locus_tag="ACHKIR_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001240072.1"
                     /note="in Escherichia coli this inner membrane protein was
                     found to anchor the periplasmic catalytic oxidoreductase
                     YedY; sulfite oxidase activity not demonstrated; contains
                     heme; frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoxide reductase heme-binding subunit YedZ"
     gene            19690..20160
                     /gene="accB"
                     /locus_tag="ACHKIR_003350"
     CDS             19690..20160
                     /gene="accB"
                     /locus_tag="ACHKIR_003350"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFO9991036.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            20171..21520
                     /gene="accC"
                     /locus_tag="ACHKIR_003351"
     CDS             20171..21520
                     /gene="accC"
                     /locus_tag="ACHKIR_003351"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFO9991037.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            21629..21871
                     /gene="yhdT"
                     /locus_tag="ACHKIR_003352"
     CDS             21629..21871
                     /gene="yhdT"
                     /locus_tag="ACHKIR_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HFO9991038.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            21861..23312
                     /gene="panF"
                     /locus_tag="ACHKIR_003353"
     CDS             21861..23312
                     /gene="panF"
                     /locus_tag="ACHKIR_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015233 - pantothenate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015887 - pantothenate transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HFO9991039.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFIGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTIVLLIGVVHAAGGLSNAVETLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRTVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            23324..24205
                     /gene="prmA"
                     /locus_tag="ACHKIR_003354"
     CDS             23324..24205
                     /gene="prmA"
                     /locus_tag="ACHKIR_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFO9991040.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            24534..25499
                     /gene="dusB"
                     /locus_tag="ACHKIR_003355"
     CDS             24534..25499
                     /gene="dusB"
                     /locus_tag="ACHKIR_003355"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFO9991041.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            25525..25821
                     /gene="fis"
                     /locus_tag="ACHKIR_003356"
     CDS             25525..25821
                     /gene="fis"
                     /locus_tag="ACHKIR_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HFO9991042.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            25907..26791
                     /gene="yhdJ"
                     /locus_tag="ACHKIR_003357"
     CDS             25907..26791
                     /gene="yhdJ"
                     /locus_tag="ACHKIR_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFO9991043.1"
                     /translation="MRTGCEPTRFGNEAKTIIQGDALTELKKLPAESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAVLVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAVANGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            26875..27054
                     /gene="yhdU"
                     /locus_tag="ACHKIR_003358"
     CDS             26875..27054
                     /gene="yhdU"
                     /locus_tag="ACHKIR_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HFO9991044.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLATETDKCRNMNSVNP
                     LKLVNCDELNFQDRM"
     gene            complement(27057..27719)
                     /gene="acrS"
                     /locus_tag="ACHKIR_003359"
     CDS             complement(27057..27719)
                     /gene="acrS"
                     /locus_tag="ACHKIR_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HFO9991045.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTLNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQDHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDEMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIIDGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHKTNELS
                     VI"
BASE COUNT         6779 a         7385 c         6958 g         6678 t
ORIGIN      
        1 gtgtatactt gattttgtga tatgggtcac gaaacaaagg cccagctaaa agattatgtc
       61 gaggtaaaaa tcatgaaaat caaaaccact gttactgcat taagcgtact ttctgttctc
      121 tctttcggtg cattcgctgc cgactccatt gatgctgcac aagcacaaaa tcgtgaagca
      181 atcgggaccg tatccgtaag tggtgtggcg tcttcgccaa tggatatgcg tgaaatgctg
      241 aacaaaaaag cggaagagaa aggcgcaacg gcctaccaga ttactgaagc tcgtagcggt
      301 gacacctggc acgctacggc tgaactgtac aaataaaccc tcatcgtctt gtccgacgat
      361 attgcccccg gttcgggggc tttttttgcg ctaatgacga acattaaaac gcaaatgccc
      421 ttccagctct tcctctgcct catcaaacag caatattaac gcgccaaaac ggcgacgcgt
      481 tttctctccc agatgaacaa attcaatctc aagtggtagc ggcaggacat cgttcattaa
      541 cacatcccat agtgagtcga gatcgcgtac cttatctttc gccagaccaa aggcttggct
      601 gaagtcgcga taaaaatcct cctgactctc aatctcatca aaatcaaagg tataaatatt
      661 catctgttgc caccgtcacg tttcacggcg agttctgccg ttcatcgcta agtatagcca
      721 tgaaaaaacc gacgcttcgg gcgtcggttt tgacttaact atcggtcaac gcatgttgat
      781 acttatggag catccccgcc agccgatgaa ccggttccgt cacctgcggt ggcgcttgcc
      841 agatgcgtag cttttcctgg tagatttcaa gttcttccag caactggcca aagtaccgac
      901 gacgtttatc atcgctacgg gcagatatca catggtctgc tgtgcggcgc atttgtcggt
      961 gaaacgccga taaatcctcg ttaaccggga tcggcgcatc acgcaggcgc tggtgcgcga
     1021 taatcatcgt cagcgccagg cgaaatttcg gcaaatcccc tgggaactta ttcatcagca
     1081 aaaacagctg ctgataaagt gccgggaggt gattctcttt acgacgtgcc acattggtag
     1141 tcatcgcgga aacagcggca gaaacaaact gattaagcag tacacgtcca gtcctgtcgc
     1201 gcgatttatc ccgcaccagc aaaataacgg tgaacgcgag cacacagccg acgatttgcc
     1261 ctaatgcgct gtcgagaaac tgactgaaat ggaaagtcat cgggttatcc agcacgataa
     1321 tatttatggt gctggccagc gcccccatcg agcccagtcg ccgtttctgt acttctatac
     1381 cgaggaaaaa tcccagcact gccaggctaa tacacagcaa taacatgctt tgttgggtat
     1441 tagggataat caccaaaaag tagagcagcc ctaacggcag cgccgccagc gtcccataga
     1501 taaagtcgat cgccaccatg cgtggattcg gcaaacgcat tgccagtgac gtcacgaccg
     1561 caatcatcac cattgcacca ctgccggaag tccagcccgt ccacagccag aaaagcgtcc
     1621 ccagaatgca ggaaagtgtg gttcgccaga agttaaccat cgcatgatga cgttcggctg
     1681 actctacttt gacttccggt tcgccttgca ggatctcttc ttcagtagcg ttgatttttg
     1741 tgttactgat aacgccgcgc ttgagaagct gataacgcgt tgccgccgcg acccagctat
     1801 aaatggtgac aggcgtttcc cgttccccgg tccaggcgat aactctccgc aggcgtttga
     1861 gctgcttgtg gacgtcctgc gcggtttcta ccggcgtgtc aaaaaattcg cggaaagtat
     1921 cagtgatcaa ttccgggcgc gtattctgaa taagataagt ttcgcaggat tgggtaatca
     1981 gcgtcagcga tagcgtattg atcgctttta aacgtcgatt ggcccgcgcc cagcgggaag
     2041 attccatatt caggttgctg cgcatacctt gtaacgccgt ggtgcgtcgc accagatcgc
     2101 cccaggcttt atcgacaact tcaccatcgc catgtttgat acagagttgc attaattgat
     2161 attgcgcgac cagcaaactt tccagctctc gatccacttc ttgtttgatc gatcgcggag
     2221 aaaagagcaa atccgccata atcgcacaca caataccgat aacgatctcg ctacaacgtt
     2281 cgacggcaaa ctgcggcgta agcaatggtt ccggctgaat ggtgatcaca atgatcagcg
     2341 cggtataacc ggccagcccc cacgcatacg agttttctat tcgtaccagc gaggatatcc
     2401 aggtacaaaa accggcccag atacagcaca ccagaatcat caataatggt gcgcggatca
     2461 tcgcaatgat gattaccagt ccagcaatac agccaataaa tgtgccgatg atgcgcaaaa
     2521 agccacgata gcgaatagcg ccagaatacg gttcacctcc cgcagcaaag gccggaccgg
     2581 cggcaacaat cgccgctgtc agtaccgccc agcgtggcgt ttccagctgg aagtgaaagc
     2641 caacaaacag cgccagtaca atggcggtcg ccagttttac cgcaaagcga atatgttgat
     2701 tagcaatgga gaaaataccc atcgtgatta accaaactca cgcaggcgat gggccatttt
     2761 acggaagaac gaatcctggc tttcgtcgcg atcttgtttg ccagtgacca ccactgtagc
     2821 agtggtgccc gcaggccaga tgttctcttg ctggttgtcg agacgaatac gtaccggaac
     2881 acgttgcgcc aaacgtaccc attcaaggtt agagtcgatg gtcgccatcc ctttgtcgtc
     2941 acgcgtgctg ctggcgttgg tgacccctgc ggcaacacta tcaacagtcc ctttcagcac
     3001 tttgttactg ccaagcggcg tgatctccgc acgataccca gggcgcaccc cttccagctt
     3061 agtttcttcc atataggcca gtacatagaa ggagttctgt ttcaccagcg caaccgccgt
     3121 tgatcctcga gtaataaact caccggtata gacgttgagg ttggtcaccc agccatctgc
     3181 tggcgcgcgg atgactgtac gttcaagatc cagttttgcc agatcacggg tcgcctgcgc
     3241 tttcgctaac tgatgcagaa ccgtttgtag tacgttgttg gcctgatcga tctcttcgcg
     3301 agacatcgcc tgcacaccga gacggttacg acgtcctgcc tcctggcgtt tctcctgtgc
     3361 cagtacctga taataagcaa catcggcttg cgcttcctca agcgcctttt gatagcgcgg
     3421 ctggtcgatg gtgaacagta cctgtccttt tttcaccagc tggttatcat gaacattcac
     3481 ctgggtaatg agtccagaaa cgtccggcgc gatcgcaacg acgtcagcgc taaagcgcgc
     3541 gtcacgcgtc cacggggatt cggtgtaata gacccaggca ttaaaaattg cgatgaaggc
     3601 cagaatgact aatacgaccg tgatggccgt acgggagaat tttcttatta gtgttttcac
     3661 ttcaacctca aacgaacagt cgcgatatca aataaaacaa gcagcaatag agcgcggtgt
     3721 tgaacaacgc cggatgccag acaaagtcgt agatacctgt tggcacaagt acccggcgca
     3781 ccagccagaa aatcgccagt gataaaagca attcaaaaaa tatcggtggg aaggacagcc
     3841 caaacaccac gataacggga aacagactca tgttgacctt ggttgtatag agagagcagg
     3901 cgttattatt ttcagcatct gtcgccgcag agaagggcat ggaaagccgg gcgagagcaa
     3961 cattgctgta gattgatatt taatatatta gcgtaactgt tatgctgtta tctatattat
     4021 gtgatctaaa tcacttttaa gtcagagtga ataatggaac gactaaaacg catgtcggtg
     4081 tttgccaaag tagttgaatt tggctctttt accgccgccg ccagacagct acagatgagc
     4141 gtttcgtcca tcagtcagac ggtgtcaaaa ctggaagatg agttacaggt aaagctgtta
     4201 aaccgtagta cacgcagcat tggcctgact gaagccggca gaatttacta ccagggctgc
     4261 cgtcggatgc tccatgaagt gcaggatgtt catgagcaac tgtatgcctt caataacacc
     4321 cccatcggga cgctgcgcat tggctgttct tcaactatgg cacaaaatgt tctcgccggg
     4381 ctgacagcaa aaatgctgaa agaataccca ggtttgagcg tcaatctggt taccggaatt
     4441 cctgcccccg acctgattgc cgatggtctg gatgtggtga tccgcgtcgg cgcgttgcag
     4501 gattccagcc tgttttcccg ccgtctgggc gcgatgccga tggtggtgtg cgccgcgaaa
     4561 agctatctca cacaatacgg cataccggaa aaacccgccg atttgagtag tcattcatgg
     4621 cttgaataca gcgtgcggcc cgacaatgaa tttgaactga tcgcaccgga agggatctcg
     4681 actcgcctga tcccacaagg aagatttgtg actaatgatc cgatgacgct ggtgcgctgg
     4741 ctgacggcgg gtgccgggat cgcctacgtg ccgctgatgt gggtgatcaa cgagatcaat
     4801 cgtggggaac tggagatcct gctgccgcgt tatcagtcag atccacgccc ggtttatgcg
     4861 ttatataccg aaaaagataa gctgccgctg aaggtacagg tcgtgatcaa ctcgctgacg
     4921 gattattttg ttgaggtcgg taaattgttt caggagatgc acgggcgcgg gaaagagaag
     4981 taattcatat tgtactgtta cgttgtacaa acctgtgcca acgggttccc ctcaccctaa
     5041 ccccagagga gcgaggggac cgttcgtgca cgtagaaaga ttaattatcc ttctgaaaat
     5101 actgaaatta ttacgcagta ccgccaacag tcaggttatc gactttcaac gttggctggc
     5161 ccacgccaac cggcaaactt tgcccttctt taccgcagac acccacgccg ttatccagtt
     5221 tcaggtcgtt acccaccatc gaaatctgct gcatggtttc gataccggaa ccaatcaacg
     5281 ttgcgccttt caccggcttc gttactttac cgttttcaat cagatatgct tctgaagtgg
     5341 agaaaacgaa tttgccggag gtgatatcca cctgaccgcc accaaagttc ggtgcataga
     5401 taccgtactc aacggattca ataatttcct gcggggtcga tttacccggc agcatatagg
     5461 tgttggtcat acgcggcatg ggcagatggg cgtaggattc acggcgaccg ttgccagtcg
     5521 gcgtcatccc catcaaacgc gcgttgagtt tatcctgcat gtagcctttc aggatgccat
     5581 tctcaatcag cacgttgtac tggcctggcg taccttcgtc atcaatcgcc accgaaccac
     5641 ggcgatcgac catcgtaccg tcatcaacca cggtacacag ttctgaagcc accagctccc
     5701 caacctgtcc actaaatact gaagtgccac ggcggttgaa gtcgccttcc agaccgtgac
     5761 ctaccgcttc atgcaacagc acgcccggcc aacctgcgcc aagtactacc ggcatggtgc
     5821 ccgctggtgc agcaacggca gaaagattga ccagcgccat acgcactgct tcttttgccc
     5881 atgcatccgc acggacttcg ccgtcgagat cggcaaggaa gaactcataa ccaaaacgac
     5941 cgccgccgcc actggcaccg cgttcgcgtt tgccatcttc ttcgaccaga acgctcacgg
     6001 aaagacgcac cagcggacgg acatccgccg ctagcgtgcc gtcggtggcc gcaaccagaa
     6061 ttaattcata gacaccactg aggctggcag tcacttcctg tacgcgcttg tccgcttcgc
     6121 gggcaacctt atcgacgcga cgcaggatat ccagcttctc ttcacggctc atgctttgca
     6181 gcggatctac cgaggtatac aacgggctat gctctaccgc gcccagcgtc tgtactttac
     6241 catcaccact atcacggacg atggtgcgcg ccgcttgtgc actctgttcc agcgccagca
     6301 ggctgatttg gtcagcataa gcaaatccgg ttttttcacc actgattgca cgcacgccaa
     6361 cgccctgatc gatgttgtaa gaaccatctt taataatacg gtcttctaaa acccaggatt
     6421 cgtgatagct cgactgaaaa tagagatcgc cataatcaag gcgacgttcg gccagttgac
     6481 cgaggatcgc gaacaagtcc tgatgtttca ggccgttcgc cgctagcaat tgttcactta
     6541 ccaggttcag actcatcgtt tttgctactc gttagttact gcagtagagg attttttacg
     6601 gctgccggat gcggcgtgaa cgccctgtcc ggcctacggt tatgttctgg ctcgttggcc
     6661 tttggcaacg attatcctat gagattgggg caattacgcg ccctcgtcaa atcattgcgc
     6721 tttttcttta cgcggttgtc gcaacacttc gttgatttgc ggatcgtcca gcggacccga
     6781 aatgtgatag cgcaaaatgg agactttgct ccacagcggt cccagcactt tactggcggc
     6841 aaacactgcc gcgccaacaa tggggttaac cgcaaaggcc gcagccacac ccaccgtcgc
     6901 agaaatctct ggtgcgacaa ccgcttccat attcaggtcg cgacgtacca gatttaccga
     6961 ccctttcata gcgatgtccg cttccaggcc atccaccagc gtgtcgtcgg tgtgcataac
     7021 gccgtcttta atccacgcgg tgctgcgaat ggagtcaaaa tagaaccctt cgccaaaagt
     7081 gtctctgaaa tcaaaacgca gcttacgcat cagggcatct acgctcaata agcgcagcaa
     7141 ttgcccggca tgtccggtat tgatttcggt aatttcgcct ttacccagtt gagtatgaat
     7201 gatgccattc aacgtcgcct catctggttg ccagggtgct ttgcgccagt gtaaatcgta
     7261 atccacatta aatgacgact ggcgtatggg cgtcgtgaca ccaaaaaatt ctgcggcggc
     7321 atcaattttc tgcccacgca gttttccttt cagcgaggtg cgttcattcc ccggattgtt
     7381 aacccattca ccatcggcag tgagccgcga aaaaccagta tcaatcagtc cattagtcag
     7441 cgttaatgta ttgccagaaa tggtgatatc actgtcaatg cgcccgaatt tttgccccca
     7501 gaaccagcac tctgcgcatc gtatttgtgc gtccggccag ccacggaagc taatgcgctc
     7561 cgttgtcggg aaaggtgatg aattcgttga gtcaccatga gttttcgcca cgcttgggtt
     7621 gtaataaaga tatttgatat tcgccagcca cggcgcgtta ttacgcatcg ccagcgtggc
     7681 gttgatttca cgcccttgcg cctcaaccag ggtgccattt gccgtcggtt gcgaaacaat
     7741 gctcaggtta ttccattgct gatttcccag cgacaacata ggcgtacgta acgttatgtg
     7801 ttgtgggaaa ctcgctgcac caccgacact ctccgcagcg cctttctgga acagggccag
     7861 ccactcggca ccattcatcg gcggcatatt gagttcaacg ccactttgtt ccggcaacgg
     7921 cgggagcgtt ttactgtctg ccgcccaaat agcacggtcg agcgtcagct tttgaccgag
     7981 caaccagcgg ctattgaaat ggttatctgc accagcctgt ccggttaact caaagctgtt
     8041 gagattgcca tcaaccttaa cgtttaccgg cagtggttca cccgcaggtt tggctaacgg
     8101 tgaaggtaag tgactgctca cattcttcag gtcgccgttc agctctacgt tataggtcgc
     8161 gccagcatga taaggcagat caatgcccac tttaccatcc cacgccacgc tgccacttaa
     8221 tgcttcgttc accgcttcag gcagaacgcc ggttttcgct ggttgccagt taccgttgag
     8281 attcaccgcg acctggtagg cttttgcccc ttctttggtg gaaaaatcca cgttcaacgg
     8341 ctgattaaac cagcttgctg tcagtggttc actttgcaga tcgccattga taaagctgaa
     8401 tttaccgctc aaatttttca gggtgctgtc gagtggtttg ataaacagac tgttattacg
     8461 cagcgtcaca tcacctttcg cggttaccag ttcgccgttc agcgggatat caagatgtaa
     8521 gcgagcattc acatcgccgt cgagctggag ttcttgcagg gtcgcaccca gagaatcttt
     8581 cagcggtgtc tcatcaaagt aagggccaac ggctttaccc ggacctttaa tgtcagcgtc
     8641 aatcagcagt ttttcttttg agtagtcagg gatcactgcg gtgagattac ttgcgcgcac
     8701 gccgcccaga ttaacgccat cggttttcat ccataaaccg tcgttaataa agtccagttc
     8761 aatatcaagg ttagttaatg caggccagtc cggctggaag gcaaacttcg cgttgcgcag
     8821 cggtaccagc acttcaaact gaccttcgtt gtgtttatag gggaagagtt gcggattgcc
     8881 accataaacc agcgtcgcgt tatccgcttc accgccctga atcgcgccac ttaagtaatc
     8941 aaccaggtct ttacccatca agttttccgg gaaatagcgc caggcttgtg aaccatcatc
     9001 ggtactgatg ccagccagaa tacccagcca gggttcatcg ttagctggtt gcaggtaacg
     9061 aaaaccgccg cgcgcatgga cggctttggc tttaacgtca atattacgcc catccagctg
     9121 gaaacctttg tcattgttca gccagcttat cgttgcctgg ccgtcggcga tttccagtgg
     9181 cgcacggaat accgtttcgt aaggcatctt tgcctgcttc atcgacgcag taagcaaacc
     9241 attttctacg ctgccggaaa gcgtcccgga gaagtgttcc gcacccggta ataatttcca
     9301 ttgcttccag gccagatcgc tccacgatgc ctgaaaacgg gtcttgtctg ccgcctgaag
     9361 cgggatatcc agcgccagcg tgttaatctt gccgctcggt tgtgtggagc gccaaacatc
     9421 acccagtgca ggtgaaagtt tcgcggccag cgggcgtatg ccctccaggc ctgccagctc
     9481 cagattactg gcgcgaatcc ggagttcgtc actgcgttta ttgtctttgc cgccaacgtc
     9541 ctgttccggt atccaggcca gcgtcaatgc tccgctcggc cagggtttgc cgtccatcgt
     9601 gatccgtgta tcgggaatag agaactgcca gcctggattt tcacgcgtaa tatgcgcggt
     9661 cagattatcc accgacagcg tatgcgtttg cttctcgccc aaccagctgg caccgccctg
     9721 tttcagccag acgtcaccgc cggttacatc gcctttgtcg atcgtcatcc agccttcaag
     9781 ggagaactgt gccgtttcca gcgcaatatt gtcctgcatc catttaccga gccacggctt
     9841 caggtcgatg tcatccgcct ggagccagac gcgaccattg cttaacaacc cctcatcatc
     9901 gcgcaaatcc atgcgcacct gcatcacgcc gtgctgtccg gtaaggctgg agaggcttac
     9961 ctggccttct gcacggtgtc gacgtggatc gttcagccag gtgagctgtg ggatcgccag
    10021 ctcggcgcgc tgaccggatg gcgtcaggaa actgacttca ctgtcgcgga gatcgaaatg
    10081 gtcgaactga cgaagaaaca gatcgctgat gtgactggct tccagactat cattaccacc
    10141 gccgctggtg ataggagtgt tggtgcgaaa gcgcagctgc cagaaagtga ggtcgcgaaa
    10201 ctgccagcgc atatgtaaca ggctctgcca gacatccagc gccagagtaa cgcgtttaac
    10261 cgaaaattcg ccgccatctt ttagttctgc acggatgtcg tgcgcttcaa gcgtcggacc
    10321 aaaattctgc cagctggctg agagctgact ggcttctacc ggcatgccag tcgcggattc
    10381 tattttgttg aggatttccg gacgccaggc gtcaagatgc ggtaaagcaa tacgcaggcc
    10441 gctaaccagc agggcagcga tcacaacgag cgcggctcca gtaagcagta aaatccccgg
    10501 caatcgcctc acccgtcact ccttgtctgc taaaaatgtg actcaaaaac cctttgccgg
    10561 atggcggccc agcatctgtt tacatcatta cgacgtcaaa ctgctcctgg ttatagagcg
    10621 gttcaatttg tactttaacc tgtttaccaa cgaaaatttc cacttccgcc agcgagtgtg
    10681 actcttcgcc tttcaaggct tcagctactg ccggagaagc atagaccagg aaacggtcgg
    10741 aatcataagc atggtgtaca cgaacaatct cgcgcatgat ttcatagcat accgtttcca
    10801 cggttttcac cgttccgcga ccgtggcagg ttgggcattc gttacatagt acgtgctcaa
    10861 tgctttcgcg ggtgcgttta cgcgtcatct ccaccagccc cagcgccgaa aaaccattaa
    10921 cgctggtttt cacccggtct ttgctcaacg cctgctccag cgagtgcagc acgcggcggc
    10981 ggtgatcttc attattcata tcgatgaaat caataatgat aatcccgccc agattacgca
    11041 accgtaactg gcgagcgata gcctgcgtcg cttcaatatt ggtattgaaa atggtgtcgt
    11101 ccagattgcg atgaccgaca aacgccccgg tattgatgtc tacggtggtc atcgcttcgg
    11161 tctggtcgat aatcagatag ccaccggatt tcagttctac tttgcgttcc agcgctcgct
    11221 gaatttcgtt ttcgacatca aagagatcga aaatcggctg gcgtccggtg taatgctcca
    11281 gcttgctggt catctcggga atgtactccg aggtaaactc cagcaatgct tcgtaagtca
    11341 ggcgtgagtc aacgcgaatg cggtcgagtt cggcgtcggc gaaatcacgc agaacacgct
    11401 gcgccagcgc cagttcgccg tacagctgat aacgggtctg tgggcgcttt ttacgctcca
    11461 taactttggt ccagacgcgt ttcagataag cggcatcgga ggccagttca gcctcgccaa
    11521 ccccttcagc tgcggtacgg atgataaacc cgccctgctc gtcgcaatac tctgcgacca
    11581 cttttttcag gcgttcacgt tctgattcgc tttcaattcg ttgggaaacc ccaacgtgag
    11641 aagcccctgg cataaatacc agatagcgag aaggcagcgt gatatcggtg gtcaggcgcg
    11701 cacccttagt gccaagtgga tctttcacca cctgcaccat cagatcttgc ccctgacgaa
    11761 ccagttccga gatgtcgcgc accgtgaatt gcttttgttc ttcacccgcc acacattcgg
    11821 tgtgcggcat gatgtcagat gcatgaagaa acgcggcttt atccagccca atatctacaa
    11881 aagccgcctg catacccgga agtacacgac ttacacgacc cttgtagata ttgcctacta
    11941 ttccgcgtcg cgcctcacgt tcaatatgaa tttcctgcag aataccgcca tcaatatacg
    12001 ccactcgcgt ttccgaaggc gttacgttta ctaacaattc agccgtcatg tttatccctt
    12061 ttctcacgca gtgcgttaaa attacttaat aattcatacg tttcaaccag cggtaagccg
    12121 actacggcgt gatagctgcc atttatcttc ctgacaaaac agccacccag cccctgaata
    12181 ccgtatgcac ctgctttatc taacggttca ccgctggcga catagcccgc gatgtcttcg
    12241 tctgttaacg ttctgaaagt cacatcggtg accaccaggc aatcgagaat gtgctggctg
    12301 tcggccaacg ccactgccgt cattacctga tgggtctgac ccgataattt gcgcaacatc
    12361 tgcgccgcat gctctgcgtc gcgcggtttc tccagtactt ctccgttcag gataacgata
    12421 gtatccgcac ccagcaccgg gagatcctgc gccgtttgcg cgacacccgc ctgtgctttc
    12481 tcgcgcgcca gacgcacaac atactgctgc gcgctctcct gcggctgacg ctgctcctca
    12541 atgcccgtaa caatacgttc aaaggttacg ccaagttgcg caagtaactc ctgacgacgc
    12601 ggagaaccgg aagctaaata cagagaagtc atagaaacct ttattgcact gcaaactgct
    12661 gacggacttt acgcatcagc aagaaaatcc acggccagag caccccattg actacgctac
    12721 tccagaacac ttccggtctg aaagagacgt taatcactaa aaactctgcc cagaaaacaa
    12781 taatatccac caccagcgaa agcaacatga cgaccagcgc ctgctgccat aatgcgaggt
    12841 tgcggaaaag ctggtatttc agcgccacca ggtaagcaat gatgctcatc gccaatacgc
    12901 gtacgccaag cgtcgagccg ctgatcagat ccagtatggc acccatcaca aaacctgtgc
    12961 ccacatttac gcgatgaggc aaggccagga tccaatacaa caagatgagt aacacccagt
    13021 ttggccggaa aacaatcagg ttatccggcc agggcatgat ttgcagcaac agcgcaatga
    13081 ggaaagagag ccagattacc cagcgtccct ggctacgata gctcgccact attgccctcc
    13141 cggcgcacgc gcaggcggtt gagcaccact ttgcggcggc gtagcccttt gtggagagcg
    13201 attagcagcg ggctgtgtgg gcgcagcagg cgcagtaact gcatttcctg tcgccggttg
    13261 ctgcggagtc ggctgagtga tccccgttgc cggttcaggt aactttggcc ccatcgcgtc
    13321 tggcgaaggc aatacctgcg gcatcatctg catcaaacgt tcattagcaa cacgatgcac
    13381 ctcttccggc gtcatcgggt tagcgccgtt acgatccgcg ccccacagca gcagcagata
    13441 acgcaaacgt tgcagtcctg cagtcggacg cgcctgaatc acagtataag cgcgctgggt
    13501 atcgagtttt acggaagaga caaccgcgac cggatagcct tccgggaaac gaccgcccag
    13561 accggaagtc accagcacat caccaacacg aatgtccgta ttcgctggca gatgctcaag
    13621 ctgcaaatca tccgtacaac cgttaccggc tgcaattacg cggatatcgt tgcgcagcac
    13681 ctggattggc agcgcgtggg tcgcatcaca aatcagcagc acgcggctgg tcagtttagc
    13741 gacggccacc acctgaccaa caacaccttt gtcgctgata accggctggc cttcataaac
    13801 gccattaacg ctacctttat cgataacaac ttgatcgcta taaggatcgt taaccgtgga
    13861 gataacctga gtcaccattt tctgctcatc ctgacgcagc ggggaaccca gtagctcgcg
    13921 cagacgcgcg ttctcctgtt tgtattgtcc aagcatcagc agttcactgt ttttcagcaa
    13981 cagttcctga cgtaacgccc ggttttcaag ttctagttgg tcacgcgagg ccagcgtctg
    14041 cgatacgcca tccagcaatt cacgaggagc attggaaaca aagtagaaag gactgacggc
    14101 ggtatccata taagtacgga tttgactgaa cgtccccagg cggctgtcgg caataataat
    14161 gccgagcgcc accagcaccg ccagaataag gcgaatctgt agcgacgggc cacggctaaa
    14221 aattggcttc ataagttatg cgtattctcg tatcagacca ggcagggtaa acagacactt
    14281 cccctgcctg catccgatta ctcttcgctg aacaggtcgc cgccgtgcat gtcgatcatt
    14341 tccagcgctt tgccgccacc gcgcgccaca caggtcagcg ggtcttcagc aacaacgact
    14401 ggaatgccgg tttcttccat taacaaacgg tcaaggttac gcagcagtgc gccaccaccg
    14461 gtaagcacca tgccacgctc ggagatgtcg gaagccagtt ccggcgggca ctgttccagt
    14521 gcaaccatta ccgcgctcac aataccggtc agcggttcct gcagtgcttc gaggatttcg
    14581 ttggagttca gggtaaagcc gcgtggaaca ccttctgcaa ggttacggcc acgaacttcg
    14641 atttcacgta cttcatcgcc cgggtaagcc gaaccgattt cgtgcttgat acgttctgcg
    14701 gtggcttcac cgatcagaga accgtaatta cgacgtacat agttgatgat agcttcgtcg
    14761 aaacggtcac caccaatgcg cacggaagag gagtaaacca cgccgttcaa ggagataaca
    14821 gcaacttcag tagtaccacc accgatatca accaccatag aaccggtcgc ttcagaaacc
    14881 ggcaggccag caccaattgc ggcagccatc ggttcttcaa tcaggaagac ttcacgggca
    14941 ccagcgccct gcgcggattc acgaattgcg cggcgttcaa cctgggtcgc gccaaccggc
    15001 acacaaacca gaacgcgcgg gcttggacgc ataaagctgt tgctgtgcac ttgtttgatg
    15061 aagtgctgga gcattttttc agtcacgaag aagtcggcga taacgccgtc tttcattggg
    15121 cgaatagcag caatattgcc cggcgtacgg cccagcatct gcttcgcgtc atgacctact
    15181 gcagctacgc ttttcggtga accggcacga tcctgacgaa tggccaccac ggaaggctca
    15241 ttcaatacga tgccttgtcc ttttacataa atgagggtat tcgcagtacc caggtcaatg
    15301 gacaagtcat tggaaaacat gccacgaaat tttttcaaca tactaaggga taatcctgaa
    15361 agctggggcg gaaaagaaaa tccgcttact ttaccaacca cacgcagcag cgacaaggcg
    15421 caaaaatcat ctgctacggt gaaaattagt gcagttcgtt tcctttgtta caaatctctg
    15481 cctgagtcca gaaaggctta atgcatcagc agcattcctc gcctgtttgc aaccgcgtaa
    15541 ggtcattcat ctgcatatgt gctgcaacaa tctggcgagc agacaagcac actcccatga
    15601 gacgcagcgc gcgttattct acgtgaaaac ggattaaacg gcaggttaaa ccgagtatct
    15661 ttgtgaatat tttttcacgt tagtatcaag tggctgtgag gacgcgaaaa aatccccttg
    15721 cccacctgta acaccgcgct gaatcagggt ctgccactcg cttcgcgaac gcacgccggt
    15781 ggcgtaaacc tgggtgctgg tcccggagca ggcttccacc aggctttgaa ccagcagctg
    15841 attctccgtt cgcttctcaa tgtttctgac cagccccgga tggagcttga gtaactcaac
    15901 attaagttct ttgatccaac tggtgcttac cagcgtcaaa ccagcctggt tgacggctac
    15961 ccgtacccct aaagcattca ctaaacgaat aacaggttgt aagcgactga tatgttgacc
    16021 aacatcggcc tctgcaagtt caataattat gcgtcttcgt tgcgattttt cacattgcat
    16081 taacgtatcg cgcagccaac gctgaaaacg cgggcgaatc agcgactcaa cggtaacctg
    16141 aatcgccaga ttttcctctg gccagtaacg caatagtgga ataagacggc tgattagcag
    16201 gcggtcatac tcttccgata aaccaaactg caagaccatc ggcatatact ctgccgagct
    16261 aacctcttca ttaccatcga agatgcggca catgagttca cgatgatgaa cccggccttc
    16321 gcgagtaacc gccggttttt gataaaggcg cgggccgccg cgactgagca tttgctcgat
    16381 aagcgtacgc cagcgaacat taccgcgtcc tttttcaggc aacgagtcat cgtaaatagc
    16441 ccagctattg ccgccctgca atcccgcatt acgcgtggca gactctgcat gttccattac
    16501 ctgctcggta tcctgaccac tacgccaggc acagatacca atgtggatca tatcgtcgcg
    16561 atcgagcatt ttattgttcg gcaaggtatc gacggctttg attaactgac tggcgatgct
    16621 ctctgcctct tttaacgtcc ggtgcggtaa cagcgcagca aaatcactgc ggtggtaacg
    16681 cgccagcagt gcgccagggt agcgcatcat aaatgtcgat agcagattcg tcagagagaa
    16741 gaactgttct tcaacctggc tgtgccccca ggtatcactc aacatattga aatccggcag
    16801 acgaatcatc atcacgatcc cgtgggtacc tactttctct tgatcttcca gtaacgttgc
    16861 taactgatta tcgaaaaaga gtcggttatt gaggccggtt ttcatgtcct gggcggcata
    16921 agagcggatc agcgtatcaa gacggctgtg ttgttcgcgt gcgttctgaa tttcacaaag
    16981 cagcgtatcc agcgcactgc tggttctggg cggccattca tagatggttc ccaacacatt
    17041 agagccacgc tcaccgttta agatacgagt agcccgggtt tccagcaatt cttgcccggc
    17101 aagttgccgt tgtaaccagc gtaccgccag gaagagcata agaatgataa agccaatcgc
    17161 ccccgtgagc ggcgcggtag tcattaaaga atggaaatag ttgcccatcg gatcctgata
    17221 aaccagacgc agcgacatcc ccggatgctt tatcaacgga acgctcagtt cacgaaacag
    17281 atcgttggtg ccaaccggac gataactacc atttctggcc agggtataaa cctgcttatc
    17341 accatggagc aggtctacac gaacgatatc tgccgacatc attaattcgg taatttgtgg
    17401 ccttaatgtg ctgaagtcat tcgacactag gtgggtatca atcgccgtcg ccaccgcctg
    17461 aacacgatga ctaaacttat actgaatggc gttgtagaaa cttagcgaac agcccagcaa
    17521 agtcacaaaa attgttaacc cggtgagcag cgtgacaaag gccgaaaatt tcgtcgttaa
    17581 tctcatcctt gtgttaactc cgatagtgag gaagcgggca tactagcaaa tcagatttat
    17641 ctcgcaattt attgcgcttc atcggctttg cttttccatt agcgagtata gtcttcagaa
    17701 attattttcc aatccatcat gcacatgagg accacttatg caggcgttac ttttagaaca
    17761 gcaggacggc aaaactctcg catcagtaca gactctggac gaaagtcgcc tgccggaagg
    17821 cgatgtcacg gttgatgttc actggtcgag cctgaactac aaagatgcgc tggcgattac
    17881 cggtaaggga aaaatcatcc gaaattttcc gatgattcct gggattgatt ttgccggaac
    17941 tgtacgcacc agcgaagatc cgcgttttca tgccggtcag gaggtgttac tcactggctg
    18001 gggcgttggt gaaaaccact ggggtgggct ggcggaacag gcgcgagtga aaggtgactg
    18061 gctggttgcc atgccgcaag ggctggacgc gcgtaaagca atgattatcg gtactgccgg
    18121 ttttaccgcc atgctgtgtg tgatggcgct ggaagatgcc ggtgttcgcc cgcaggatgg
    18181 ggaggttgtc gtgacgggtg ccagtggtgg cgtcggcagt accgccgtca cgctgctgca
    18241 taagttgggt tatcaggtcg ttgccgtttc cggtcgcgaa agtacccatg aatatctgaa
    18301 aagtttaggt gccagccgta ttcttcctcg cgatgagttt gctgaatccc gtcctctgga
    18361 aaaacaagtc tgggctgggg caattgacac cgttggcgac aaagtgctgg caaaagtgct
    18421 ggcgcaaatg aattacggcg gctgcgtggc ggcctgtggt ctggcgggtg gttttactct
    18481 gccaaccacg gtcatgccat ttattctgcg taatgtccgt ttgcaagggg tggattcagt
    18541 aatgacgcca ccagcacgcc gcgcacaagc ctggcagcga ctggtcgccg atttaccgga
    18601 atcattctat acccaagcgg caaaagagat atctctggca gaggcaccga agtttgccga
    18661 ggccatcatt aataaccaga tccagggtcg cacgctggtg aaggttaact aaccatttag
    18721 cagggaataa taagagaggg aactcatttt gaaatcatta ttcaatcggt taacgggaaa
    18781 agcggttagc cggacagctt tcgtcgaaca tcttggtcat gaagttgtac aacatcatcc
    18841 aaactggaaa gtcatgattt cgactgacca taaattgatg cgcattgatg ctccaccaaa
    18901 cagccattat tgatacgcct ccgtcgcctg ttaggtttat ggtgttttgc cttggcgacg
    18961 ctacgcttaa tctgttactg atttctggta ccatgggggg aataatctga ttttatttga
    19021 ttgcaaatta accacttaaa cctattgaat gcggaacatt gtcagtcggt taattttgcg
    19081 tgctttagca ttcacatctt tccggacgat gcagtgaaaa ttgcgtaatc ctcagcaacc
    19141 gctgcgtaat gtcgtctatc ttgtcgcgat cctggcatcc ctacattatt tgtggtctgt
    19201 gaagattatc tcattgcagc ccctcatctt cgcagggctg gctttacagc ttttcaccct
    19261 acgttataag aagttccgtc gatgatggcg ctaatttcgt gaattgtgcg gcttgttgca
    19321 aattacacgg tgttgaaggt tatttacatg ttagctgttg attatcttcc ctgataagac
    19381 cagtatttag ctgccaattg ctacgaaatc gttataatgt gcgacctcgt cctccctgac
    19441 gcagtttttg cgctgcggaa aaggtgacat tggcgcaacg aaggtatatt ttgttttttg
    19501 ccggaggata gcagcagatc gctgcacaat gtccgtcaag tctaacattg acactctggg
    19561 gcaaaataga ccggcgtccc ggcctgctgg aatttatcgc tatgcataca gctgtcgggg
    19621 catacgcttt acagacggcg gtgaaacgcc tgtcacaatc acactaaaca aagagtacgg
    19681 aacccactca tggatattcg taagattaaa aaactgatcg agctggttga agaatcaggc
    19741 atctccgaac tggaaatttc tgaaggcgaa gagtcagtac gcattagccg tgcagctcct
    19801 gccgcaagtt tccctgtgat gcaacaagct tacgctgcac caatgatgca gcagccagct
    19861 caatctaacg cagccgctcc ggcgaccgtt ccttccatgg aagcgccagc agcagcggaa
    19921 atcagtggtc acatcgtacg ttccccgatg gttggtactt tctaccgcac cccaagcccg
    19981 gacgcaaaag cgttcatcga agtgggtcag aaagtcaacg tgggcgatac cctgtgcatc
    20041 gttgaagcca tgaaaatgat gaaccagatc gaagcggaca aatccggtac cgtgaaagca
    20101 attctggtcg aaagtggaca accggtagaa tttgacgagc cgctggtcgt catcgagtaa
    20161 cgaggcgaac atgctggata aaattgttat tgccaaccgc ggcgagattg cattgcgtat
    20221 tcttcgtgcc tgtaaagaac tgggcatcaa gactgtcgct gtgcactcca gcgcggatcg
    20281 cgatctaaaa cacgtattac tggcagatga aacggtctgt atcggccctg ctccgtcagt
    20341 aaaaagttat ctgaacatcc cggcaatcat cagcgccgct gaaatcaccg gcgcagtagc
    20401 aatccatccg ggttacggct tcctctccga gaacgccaac tttgccgagc aggttgaacg
    20461 ctccggcttt atcttcatcg gcccgaaagc agaaaccatt cgcctgatgg gcgacaaagt
    20521 atccgcaatc gcggcgatga aaaaagcggg cgttccttgc gtaccgggtt ctgacggccc
    20581 gctgggcgac gatatggata aaaaccgcgc cattgctaaa cgcattggtt atccggtaat
    20641 tatcaaagcc tccggcggcg gcggcggtcg cggtatgcgc gtagtgcgcg gcgacgctga
    20701 actggcacaa tccatctcca tgacccgtgc ggaagcgaaa gctgctttca gcaacgatat
    20761 ggtttacatg gagaaatacc tggaaaatcc tcgccacgtc gagattcagg tactggctga
    20821 cggtcagggc aacgctatct atctggcgga acgtgactgc tccatgcagc gccgccacca
    20881 gaaagtggtc gaagaagcac cagcaccggg cattaccccg gaactgcgtc gctacatcgg
    20941 cgaacgttgc gctaaagcat gtgttgatat cggctatcgc ggtgcgggta ctttcgagtt
    21001 cctgttcgaa aacggcgagt tctatttcat cgaaatgaac acccgtattc aggtagaaca
    21061 cccggttact gaaatgatca ccggcgttga cctgatcaaa gaacagctgc gtatcgctgc
    21121 cggtcaaccg ctgtcgatca agcaagaaga agttcacgtt cgcggccatg cggtggaatg
    21181 ccgtatcaac gccgaagatc cgaacacctt cctgccaagc ccgggcaaaa tcacccgttt
    21241 ccacgcacca ggcggttttg gcgtgcgttg ggagtctcat atctacgcgg gctacaccgt
    21301 accgccgtac tatgactcaa tgatcggtaa gctgatttgc tacggtgaaa accgtgacgt
    21361 ggcgattgcc cgcatgaaga atgcgctgca ggagctgatc atcgacggta tcaaaaccaa
    21421 cgttgatctg cagatccgca tcatgaatga cgagaacttc cagcatggtg gcactaacat
    21481 ccactatctg gagaaaaaac tcggtcttca ggaaaaataa gactgcaaaa gcgtcaaaag
    21541 gccggatttt ccggcctttt ttattactgg ggatcgacaa cccccataag gtacaatccc
    21601 cgctttcttc acccatcagg gacaaaaaat ggacactcgt tttgttcagg cccataaaga
    21661 ggcgcgctgg gcgctggggc tgaccctttt gtatctggcg gtatggttag tagccgctta
    21721 cttacctggc gttgcccccg gttttaccgg ttttccgcgc tggtttgaga tggcctgcat
    21781 cctgacgccg ctgctgttta ttggactgtg ctgggcgatg gtgaaattta tctatcgcga
    21841 tatcccactg gaggatgacg atgcagcttg aagtaattct accgctggtc gcctatctgg
    21901 tggtggtgtt cggtatctct gtttatgcga tgcgtaaacg gagcactggc accttcctta
    21961 atgagtattt cctcggcagc cgctctatgg gcggtattgt gctggcgatg acgctcaccg
    22021 cgacctatat cagtgccagt tcgtttatcg gcgggcctgg agctgcttat aaatacggcc
    22081 tgggctgggt attgctggcg atgattcagc ttcctgcagt ctggctttcg ctcggtattc
    22141 tcggcaagaa gttcgcaatt cttgcgcgcc gctacaatgc ggtgacactg aacgacatgc
    22201 tgtttgcccg ctaccagagt cgtcttttgg tgtggctggc gagtttgagt ttgctggtcg
    22261 cgtttattgg cgcgatgacc gtgcagttta tcggcggcgc gcgcctgctg gaaaccgcgg
    22321 cgggtattcc ttatgaaacc gggctgctga ttttcggtat cagcattgcg ttgtataccg
    22381 cctttggtgg ctttcgcgcc agcgtgctga acgacactat gcaagggctg gtgatgctga
    22441 ttggcaccat tgtgctgctt attggcgtgg tacatgccgc aggtggctta agcaacgcag
    22501 tagagacgtt gcaaaccatc gatccgcaac tggttacgcc acaaggcgct gacgatattc
    22561 tgtcgcctgc ctttatgacg tcgttctggg tactggtgtg ctttggcgtg attggcctgc
    22621 cgcatactgc ggtgcgctgt atctcttata aagacagcaa agccgtacat cgggggatca
    22681 tcatcggtac gattgtggtc gcaatcctga tgttcggtat gcacctggcc ggagcgttag
    22741 gtcggacggt gatccccgat ctcaccgtac cggacctggt gatcccgacg ttaatggtaa
    22801 aagtgttgcc accgtttgct gccgggatct tcctggctgc acctatggct gcgatcatgt
    22861 cgacaattaa cgcccaactg ctgcaaagtt ccgctacgat cattaaagat ctctatctga
    22921 atatccgtcc ggatcaaatg caaaacgaga cgcgtctgaa gcggatgtcg gcggtaatta
    22981 cgttagttct cggtgcgttg ctgctgcttg ccgcctggaa gccgccagaa atgatcatct
    23041 ggctgaattt gctggccttc ggtgggctgg aagccgtttt cctgtggccg ctggtactgg
    23101 gtctttactg ggaacgcgcc aacgccaaag gcgcgctaag tgcgatgatt gttggcggcg
    23161 tactgtatgc cgtactcgcg acgctgaata ttcagtacct gggcttccac cctatcgtgc
    23221 cctcgttact actaagtttg ctggctttcc tggtcggaaa ccgtttcggt acatccgtcc
    23281 cgcaagctac cgttttgact actgataaat aaagagtttt gccatgcctt ggatccaact
    23341 gaaactgaac accaccggcg cgaacgcgga agatcttagc gatgcgctga tggaagcggg
    23401 tgccgtttct atcacttttc aggataccca cgatacgcct gtgtttgagc cgctgccggg
    23461 cgaaacgcgc ctgtggggcg acaccgatgt gattggtctg ttcgacgctg aaaccgatat
    23521 gaacgacgtg gtggcgattc tggaaaacca tccgctgctc ggcgcgggct tcgcgcataa
    23581 aatcgaacaa ctggaagata aagactggga gcgcgaatgg atggataatt tccacccgat
    23641 gcgctttggt gaacggctgt ggatctgccc aagctggcgt gatgtgccgg acgaaaacgc
    23701 cgtcaacgtg atgttagatc cagggctggc gtttggtacg ggtacccatc caactacctc
    23761 tctgtgcctg cagtggctcg acagcctcga tttaaccggt aaaacagtca tcgactttgg
    23821 ctgcggttcc ggcattctgg ctatcgcggc gctgaaactg ggtgcagcaa aagccattgg
    23881 tattgatatt gatccgcagg cgattcaggc cagccgcgac aacgcggaac gtaatggcgt
    23941 ctctgaccgt ctggaactct acttaccgaa agatcagcca gaagaaatga aagccgacgt
    24001 ggtggtcgct aacatccttg caggcccatt acgtgaactg gcaccgttaa tcagcgtcct
    24061 gccagtttca ggcggtttgc tgggcctttc cggtattctg gcaagccagg cagagagcgt
    24121 ttgcgaagct tatgccgaca gcttcgctct ggacccggtc gtggaaaaag aagagtggtg
    24181 ccgcattacc ggtcgtaaga attaaccttc gcatcgccgt agggtgacgc agagcgaagt
    24241 gcgagcgatg tcacaaaagg cacgttaatt tgccgattat ttacgcaaat ttgcgtgcca
    24301 aaattttcat tcataaagaa aaattgagaa cttactcaaa tttcttggag tgtaaatttt
    24361 agtcactatt ttctaatatg atgattttta tgagtaatta tcgcgccacg ctcattttaa
    24421 atgcaattct ttgatccatc tcagaggatt ggtcaaagtt tggcctttca tctcgtgcaa
    24481 aaaatgcgta atatacgccg ccttgcagtc acagtatggt catttcttaa ctcatgcgca
    24541 tcggacaata tcagctcaga aatcgcctga tcgcagcgcc catggctggc attacagaca
    24601 gaccttttcg gacgttgtgc tacgagatgg gagccggatt gacagtatcc gagatgatgt
    24661 cttctaaccc tcaggtttgg gaaagcgaca aatctcgttt acggatggtg cacattgatg
    24721 aacccggtat tcgcaccgtg caaattgctg gtagcgatcc gaaagaaatg gcagatgcag
    24781 cacgtattaa cgtggaaagc ggtgcccaga ttattgatat caatatgggt tgcccggcta
    24841 aaaaagtgaa tcgcaagctc gcaggttcag ccctcttgca gtacccggat gtcgttaaat
    24901 cgatccttac cgaggtcgtc aatgcagtgg acgttcctgt taccctgaaa attcgcactg
    24961 gctgggcacc ggaacaccgt aactgcgaag agattgccca actggctgaa gactgtggca
    25021 ttcaggctct gaccattcat ggccgtacac gcgcctgttt gttcaatgga gaagctgagt
    25081 acgacagtat tcgggcagtt aagcagaaag tttccattcc ggttatcgcg aatggcgaca
    25141 ttactgaccc gcttaaagcc agagctgtgc tcgactatac aggggcggat gccctgatga
    25201 taggccgcgc agctcaggga agaccctgga tctttcggga aatccagcat tatctggaca
    25261 ctggggagtt gctgcccccg ctgcctttgg cagaggttaa gcgcttgctt tgcgcgcacg
    25321 ttcgggaact gcatgacttt tatggtccgg caaaagggta ccgaattgca cgtaaacacg
    25381 tttcctggta tctccaggaa cacgctccaa atgaccagtt tcggcgcaca ttcaacgcta
    25441 ttgaggatgc cagcgaacag ctggaggcgt tggaggcata cttcgaaaat tttgcgtaaa
    25501 cagaaataaa gagctgacag aactatgttc gaacaacgcg taaattctga cgtactgacc
    25561 gtttctaccg ttaactctca ggatcaggta acccaaaaac ccctgcgtga ctcggttaaa
    25621 caggcactga agaactattt tgctcaactg aatggtcagg atgtgaatga cctctatgag
    25681 ctggtactgg ctgaagtaga acagcccctg ttggacatgg tgatgcaata cacccgtggt
    25741 aaccagaccc gtgctgcgct gatgatgggc atcaaccgtg gtacgctgcg taaaaaattg
    25801 aaaaaatacg gcatgaacta attcaggtta gctaaatgct tgattaaaaa ggcgctactc
    25861 ggcatgggga agcgcctttt ttataggtgt cacaaaggga gtgaccatga gaacaggatg
    25921 tgaaccgacc cgatttggta atgaagcaaa gaccatcatc cagggtgatg cattaaccga
    25981 acttaaaaag ctccccgccg aaagcgtcga tctgatcttt gccgacccac cgtataacat
    26041 cggtaaaaat tttgatggtc tgatcgaagc ctggaaagaa gatctgttta tcgactggct
    26101 gtttgaagtg attgcagagt gccaccgcgt tctgaaaaag cagggcagca tgtacatcat
    26161 gaacagcacg gaaaacatgc cctttatcga tctccagtgc cgcaagcttt ttaccatcaa
    26221 aagtcgcatc gtctggtcat atgacagttc aggagtgcag gcgaaaaaac actacggctc
    26281 catgtacgaa cccatcctga tgatggtgaa agacgcaaag aactacacat tcaacggtga
    26341 tgctgttctg gtagaagcca aaaccggatc gcagcgcgcg ttgatcgatt atcgcaaaaa
    26401 tcctccacag ccttacaatc atcaaaaagt accgggtaac gtctgggatt ttcctcgcgt
    26461 gcgttatttg atggatgaat atgaaaacca cccgacgcaa aaaccggaag ccttactgaa
    26521 acgcattatt ctcgcctctt ccaacccagg cgatatcgtt ctcgacccgt ttgccggtag
    26581 ctttactacc ggtgccgtag ccgtcgccaa cgggcgaaaa ttcattggta ttgagatcaa
    26641 cagcgagtac atcaaaatgg ggcttcgacg gctggatgtc gcgtcgcatt actctgcgga
    26701 agaactggcg aaagtgaaaa aaagaaagac cggcaacctg tcaaaacgaa gccggttaag
    26761 cgaagttgac cccgatctca ttgcaaagta aaggaagtgt aagcctggtt ttcagattat
    26821 tcatttcgtg tatatttcct gccagacttg gttaaacatg cacaggctca ggtaatgatt
    26881 cgcaagtatt ggtggctcgt cgttttcgct gtcttcgttt ttctgtttga tactttactg
    26941 atgcagtgga ttgaactgct ggcaacagaa acagacaaat gccgcaatat gaactcagtt
    27001 aatccactaa aactggttaa ctgtgacgaa ctgaattttc aggacagaat gtgaatttat
    27061 atgacactta attcattcgt tttatgaatt aatttcgtta tgttttcatc tggcatgaac
    27121 attcttaata cgttatcgac cagagcgggg gcttgtttat aaagatcata acccgccata
    27181 ttcattaacc agttttgaac aattccgctg aaggcaccat caataataat catcacaaca
    27241 tctaaatcga gattatttgc tacacaacct tgttgctgac acgcctgcaa tacttcgcgg
    27301 agagtctgcg gattaaagcc catcttttcg cgtatcactc cctcggccag catctcatca
    27361 ttaaattcac atttgtgata taagattttc agcaacgcct gctggcgggg aattttggca
    27421 atatattgca aaccgacaat caatttttca cgcaattgct gaaacgggtc atgctctaac
    27481 ccagcggtca agtgatcctg gattaactcc cgcaatgaag gctgttgcaa ccacatctca
    27541 ttaaacagtt gggtcttgtt ctcgaagtgc cagtagatag cgccacgcgt aacgttagca
    27601 gcgtcggcaa tgtcgttgag cgtcgtcttg cttacgccat gttgcgcaaa ctgggcgatg
    27661 gcagtttcaa tcagttcttg ccgggtcttc agagcttcgg ctttggttct ttttgccatg
    27721 attaattatt caggaaataa atatattcga cacagagtga gaaaatagcg aaggttaatc
    27781 tatcacctaa tgtgtattta
//
LOCUS       DAZGQL010000072        27792 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.072, whole genome shotgun sequence.
ACCESSION   DAZGQL010000072 DAZGQL010000000
VERSION     DAZGQL010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27792
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.072"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..191)
                     /locus_tag="ACHKIR_003360"
     CDS             complement(<1..191)
                     /locus_tag="ACHKIR_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000994803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9991046.1"
                     /translation="MDIVLTELNKLGKASAESISKGLNIDLNDVIDTLWKLKNQGAVT
                     VKNGIWQAIAGGVGNKTNIA"
     gene            complement(227..439)
                     /locus_tag="ACHKIR_003361"
     CDS             complement(227..439)
                     /locus_tag="ACHKIR_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HFO9991047.1"
                     /translation="MSEKTTLTKASPVELRQCLEIANQLARSGIRFVPIPITADAELH
                     LFGEILSRKLDELEKLVEEGDTSPTE"
     gene            complement(399..1025)
                     /locus_tag="ACHKIR_003362"
     CDS             complement(399..1025)
                     /locus_tag="ACHKIR_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MT-A70 family methyltransferase"
                     /protein_id="HFO9991048.1"
                     /translation="MTKKYTLIYADPPWTFRDKATDGQRGASFKYPVMSLLDICRLPV
                     WELAADNCLLAMWWVPTQPLEALKVVEAWGFRLVTMKGLTWNKCGKRQTDKLVMGMGS
                     TTRANSEDCLFAVKGNLPERINAGIIQSFTAPRLDHSRKPDMAREKLVQLLGDVPRIE
                     LFARHTSHGFDVWGNQCGTPSIEMVPGIVKFLEKTNERKNDVDKGITS"
     gene            complement(1022..1453)
                     /locus_tag="ACHKIR_003363"
     CDS             complement(1022..1453)
                     /locus_tag="ACHKIR_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HFO9991049.1"
                     /translation="MKQIAFYRRSGRPGAFRGLKERVTWMIQSRGRPVTGSEIAEKFG
                     VSLCEFNKVARGLTKGSKVVKIKASEPFTTDTGIVDRFFSLESNPRRNTPRSRNAVPP
                     FSRRSREHAAKNCREEYVQKAERRRRLIKAGLYIDEFENAL"
     gene            complement(1509..2180)
                     /locus_tag="ACHKIR_003364"
     CDS             complement(1509..2180)
                     /locus_tag="ACHKIR_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HFO9991050.1"
                     /translation="MTTQISVETISPITHNQIPVITTELLAQLYGTETNNIKVNYTRN
                     AERFVCGKHYFKLEGAGLREFKNKVTQSNLVAPRTKHLILWTERGAARHAKMLETDQA
                     WEVFEKLEDCYFSQKIPEQLQLPESTLSINYPLSWFSEHHPYSMMSYVDRKTLNLDVS
                     VLFDMPSPTMRILNELHSKGYNVDAAVAEFNAFKHLTEEMRRTLQDISRLSDRNSRKG
                     FSLSL"
     gene            2508..2765
                     /locus_tag="ACHKIR_003365"
     CDS             2508..2765
                     /locus_tag="ACHKIR_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HFO9991051.1"
                     /translation="MRTTQQFSITLTNEMADMVRARVASGAYASESEVIREGLRALNE
                     RDKAMEAWLIHSAAPPLDAIRENLGKGHSISQVRSEIRARK"
     gene            2819..3091
                     /locus_tag="ACHKIR_003366"
     CDS             2819..3091
                     /locus_tag="ACHKIR_003366"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9991052.1"
                     /translation="MSLAAPIRDPIMNIIARFFYLIIFCMATSGCTTTTKINRGDEKE
                     QYIIACGAATPWGVCYDKANALCKNGYKDILKEQGFNRKELTIECK"
     gene            3181..3486
                     /locus_tag="ACHKIR_003367"
     CDS             3181..3486
                     /locus_tag="ACHKIR_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001240641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFO9991053.1"
                     /translation="MAMFNPPHPGEIIADILEDQNIGIRELARALDVAPSTVQRLVSG
                     NTTISPEMAVRLAAVLGGTPSSWIRLQTAWSLEKAEREVDVSHLSTKYRPAEISPHA"
     gene            complement(3529..4269)
                     /locus_tag="ACHKIR_003368"
     CDS             complement(3529..4269)
                     /locus_tag="ACHKIR_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9991054.1"
                     /translation="MINNIANQSSFPAATIDSQMLLKMVNEARKLCSEKPVRNNDFIA
                     RVKDELEGETYEIFVGQKNGAEIEVITMTFKQALRVAARESKAVRRSLVDQLESMQQQ
                     LQQKITSKHSTNGLEEFRKARALKMTVDTMKDLFGFLPNLAPEAKQVVAASLVNPVVG
                     ANVIPLPMINEHYYSASEVGAKLKISANKVGRIANTYMLKTEQYGKWFIDKSPYSDKQ
                     VESFRYNNRGVQKIEEILDAENKAEFGT"
     gene            complement(4318..4602)
                     /locus_tag="ACHKIR_003369"
     CDS             complement(4318..4602)
                     /locus_tag="ACHKIR_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFO9991055.1"
                     /translation="MANLQLAVKGEYFDAMIRGEKTEEYRLCNDYWINRLVNRKYDRL
                     IITKGYPKRDDSSRRIDTPYDGYKIKTITHTHFGDKPVKVFAIKVNIGSD"
     gene            complement(4595..5128)
                     /locus_tag="ACHKIR_003370"
     CDS             complement(4595..5128)
                     /locus_tag="ACHKIR_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFO9991056.1"
                     /translation="MTTITKERLLTIKQWRETYGPGSNVVLPAEEAEELARIALTSLE
                     AEPVAWKVTFTQIDREYNTFTGMYSDKAEVERWVRLHKACNFRADITPLYTAKPVPVT
                     PDGWISCSERMPEKGQNVLISVNFDSSLVEPLICSARYTGSTFRRGDATIKPGNGIEQ
                     ATHWMPLPEPPQEVNRG"
     gene            complement(5125..5490)
                     /locus_tag="ACHKIR_003371"
     CDS             complement(5125..5490)
                     /locus_tag="ACHKIR_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFO9991057.1"
                     /translation="MALTKKQRAELRMKFGGRCAYCGCELGEKWHADHVKPVIRFDGN
                     MLHQERDDISNMVPACHPCNLHKHCSSLEDYRRIISDGRREFLASGKGKALVRMGLVE
                     MKSDPVVFWFEKYQEGATV"
     gene            complement(5492..6352)
                     /locus_tag="ACHKIR_003372"
     CDS             complement(5492..6352)
                     /locus_tag="ACHKIR_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFO9991058.1"
                     /translation="MSEIDYQALREKAEKATCGEWSLEYGEGRFDGDDALIHREAAGY
                     IPICRIEGAHPESGFDEDFQMEQQANAEFIAAANPATVLALLDERERNQQYIKRRDQE
                     NEEIALTVGRLLIENGQLVADTLRHLADNEIDSDYFAITSTNENGTEIEHEMAITDYA
                     LQAAGTVDELVAALEAAEKRNAKLQRENAYIRNRYKELDLLIGKNILVMQAAIIEWQA
                     TGDAKSGLAWIYNTLFGPGELPDKSEKDAQAYFNRKYAPIDEKLMALHKWFWEQSEAE
                     RSAGIRIKGE"
     gene            complement(6349..6834)
                     /locus_tag="ACHKIR_003373"
     CDS             complement(6349..6834)
                     /locus_tag="ACHKIR_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HFO9991059.1"
                     /translation="MTGHAAILDMCCGSRMFWFDKNDDRAIFSDIRKEEHTLCDGRRL
                     IISPDLIADFRALPFADASFPVVVFDPPHLERVGDNAWMGKKYGRLNKDTWRDDLRQG
                     FKEAFRVLWPYGVLIFKWNETQIPVRQILALTDRKPVIGQRTGKGDKTHWIIFMKEGN
                     Q"
     gene            complement(6831..7532)
                     /locus_tag="ACHKIR_003374"
     CDS             complement(6831..7532)
                     /locus_tag="ACHKIR_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFO9991060.1"
                     /translation="MTTEIDYQVLRDVSERAITAMTHLSILPGDDDLLSEKKLKELGI
                     DIDAIHAFKIMAGPETVLSLLDERDALNERIAELEANLAKLAEDQQKAIESIKQADSA
                     VKLAHEKFSELAAENAALKQSEKEFNNFCRQEYYGWEDNFTETPATDAFLAEIRAEAR
                     NEGINYTASRLAAAFNHGFINKSLREVFDVTRMILSAKEELANEPYPIDGLSGEYAEK
                     SLEEWAERLRKGGNQ"
     gene            complement(7635..8078)
                     /locus_tag="ACHKIR_003375"
     CDS             complement(7635..8078)
                     /locus_tag="ACHKIR_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9991061.1"
                     /translation="MQFKDLPEDIQKIAADTLKAHLSVLDLTKEPKANLENISRNVRD
                     VFVGLYAYDNEKHEEHIQNGCINKFQQNIKLPVADKTEQKTDVLVFWNTVEAIARSTM
                     LEFNNINHSGIDTHQSSHREGFSILLTLLQEQGEYPQPTSNDTEQ"
     gene            complement(8155..8976)
                     /locus_tag="ACHKIR_003376"
     CDS             complement(8155..8976)
                     /locus_tag="ACHKIR_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdQ family protein"
                     /protein_id="HFO9991062.1"
                     /translation="MSQLDSNAIKEIVKLTTTAFSGENLPLTECPVALLPDNVNIESL
                     ERFMTERFRFRGVMTTTSIDDFVEYSKGYADECSRCFINAETMKAVTVFNIGTLEQPG
                     HADNKALLELKATSPYRALRDVDGKRQLQKSLAEWLEDWADFLTAYDSDGNVLDIKQA
                     ISAVRRLTIDAKRSAEYEEQNFSGSRSVMESVEAKSKEIMPATFRFECIPYEGLGNRE
                     FTLRLSILTSEQPVLVLRIVRVEAAEEEIAKEFRDLLKERFEEEDILTFIGTFSV"
     gene            complement(9040..9387)
                     /locus_tag="ACHKIR_003377"
     CDS             complement(9040..9387)
                     /locus_tag="ACHKIR_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9991063.1"
                     /translation="MNQHQTDVNVFINDLDGGVFVNKLGAVLSEVAFGVNSTNKKGKV
                     CVEFELSSLDENRVSVSHKLKFTRPTMRGSKSEEDTTNTPMFVNKGGELTLFQKDQGQ
                     LFDKQGQHDAVLR"
     gene            complement(9462..10049)
                     /locus_tag="ACHKIR_003378"
     CDS             complement(9462..10049)
                     /locus_tag="ACHKIR_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF669 domain-containing protein"
                     /protein_id="HFO9991064.1"
                     /translation="MDEVIFTYNEESALTAGQGSFITETGAHIINITEAELKQSEKGA
                     RFIEFSGESDDGRKIQYLSVCVQKNDGTENKFGASIIHAMMGCTGIGQLTQHMVSVSK
                     YVAPEFHGKKIGLVLQKVLTTNRKTGADGYQMEIRIPFIAETGQTLKEKAEGKKPETV
                     ANMVSTLKDKDNRSKNVNPNHTDAPGYWQYGSDSF"
     gene            complement(10049..10738)
                     /locus_tag="ACHKIR_003379"
     CDS             complement(10049..10738)
                     /locus_tag="ACHKIR_003379"
                     /EC_number="3.1.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lambda exonuclease family protein"
                     /protein_id="HFO9991065.1"
                     /translation="MTPEIILQRTGVDITSLDQGDDGWHKLRLGVITASEVHNVIAKP
                     RSGSKWPDTKMSYFHTLLAEVCTGVAPEVNAKSLAWGKQYEDDARALFEFIADVTVSE
                     TPIIFRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFDAIKPAYMAQVQF
                     NMWVTDKDAWYFANYDPRMKREGLHYVVVERDEKYMASFDEMVPEFIDKMDEALAEIG
                     FVFGEQWGVNN"
     gene            complement(10735..11685)
                     /locus_tag="ACHKIR_003380"
     CDS             complement(10735..11685)
                     /locus_tag="ACHKIR_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000459722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HFO9991066.1"
                     /translation="MFDIVEFVKQQERFFCEALTEPTLTWAKESQFAIQQFQKNAFLA
                     DTARANLPSAQNAIINVAAIGITLNPASKLAYLVPRKKAVCLDISYMGLLHLAQVTGA
                     IQWGQCKLVYEKDIYESNGIDCAPTHKYNPFVDRGARIGGYCVVKTSEGDYLTEEMSN
                     REIEVIRACSKAGNNGGSSPWDSFPDEMARKAIVKRASKYWPRRDRLDTAIDYLNTQG
                     GEGIILNADHIPERDVTPASDEIINEITQAITEINKTWDDLLPLCSKTFRRTIASHEY
                     LSQEEAVKTLDFVKKKAARNKATAEAKIHATTENNSEAVS"
     gene            complement(11702..11983)
                     /gene="gamL"
                     /locus_tag="ACHKIR_003381"
     CDS             complement(11702..11983)
                     /gene="gamL"
                     /locus_tag="ACHKIR_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000995345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host nuclease inhibitor GamL"
                     /protein_id="HFO9991067.1"
                     /translation="MNAYLTCDRIEERRWINQQIQDEKDKWIDDRAQELISMFPDKPL
                     LMSSLFLPKEAQLALTGEKAEEAYNDYISAIAYARAEEEWERKFSPCPF"
     gene            complement(12004..12285)
                     /locus_tag="ACHKIR_003382"
     CDS             complement(12004..12285)
                     /locus_tag="ACHKIR_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFO9991068.1"
                     /translation="MLNQKININVKSVVTPAGTIMGEVFMDDKIIAYFVILSDEAISV
                     IDTEGNVMFIAEHPEDIALQAAAYFFAKEQEEECNCPVCQLSRQINLMH"
     gene            complement(12297..12509)
                     /locus_tag="ACHKIR_003383"
     CDS             complement(12297..12509)
                     /locus_tag="ACHKIR_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000917251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor protein"
                     /protein_id="HFO9991069.1"
                     /translation="MLKPHYGTALVSREDVKPGTAILYNGRYYMASANVNNALYAHSL
                     IEKIRIISDAIEVYLNNKGQPLISPA"
     gene            complement(12580..13353)
                     /locus_tag="ACHKIR_003384"
     CDS             complement(12580..13353)
                     /locus_tag="ACHKIR_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFO9991070.1"
                     /translation="MGPTSVGLVPLIASSPNPVQSTASELRTSCGGSQSPIKEAAIMA
                     TIPALSQPEITITNGRVVTTSIAIAEFFDKRHDDVLKKIRLMDCSTEFNARNFADVTY
                     TDAKGEQRPMYQITKNGFVFLVMGFTGKKAAAFKEAYIAEFDRMEKQLWEQKQLALSS
                     PAMNINFPLSWFSEHHPYATMNYVDRKVISLDASVLFDMPSPTMRILNELHSKGYNVD
                     AAVAEFNAFKHLTEEMRRKLLDISRTSEKSSRFGFNVNL"
     gene            13383..13682
                     /locus_tag="ACHKIR_003385"
     CDS             13383..13682
                     /locus_tag="ACHKIR_003385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9991071.1"
                     /translation="MSIQKKTLARLYRLDCQRGPNPDTRFIRCSPNIAPTSHAVNTVL
                     SQKRFGGAACTPKHSLYLVSANFLPVLPVLTPFSLSRGDTLSSTGSHPMIQHVYV"
     gene            complement(13982..14935)
                     /locus_tag="ACHKIR_003386"
     CDS             complement(13982..14935)
                     /locus_tag="ACHKIR_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BsuBI/PstI family type II restriction
                     endonuclease"
                     /protein_id="HFO9991072.1"
                     /translation="MNNQNDYIEAAQQIIASLGLPRAQQNERSALCLLALLNLTPGKA
                     WADAENPLVGITPIMNWVREHYGKVYAPNTRETFRRQSMHQFCAAGVALYNPDKPDRP
                     VNSPKAVYQIEPAALSMLRTFGSPAWHDSLATYLAERETLVTRYAKEREQNRIPVEIA
                     AGQQITLSPGEHSELIRAIIEDFAPRFAPGSVLVYAGDTGEKWGYFDAPLLAGLGVDV
                     DSHGKMPDVVLHFTAKNWLLLVESVTSHGPVDGKRHAELARLFAGSTAGLVYVTAFPN
                     RSIMGRYLGEIAWETEVWVADAPSHLIHFNGERFLGPYSTE"
     gene            complement(14932..16401)
                     /locus_tag="ACHKIR_003387"
     CDS             complement(14932..16401)
                     /locus_tag="ACHKIR_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Eco57I restriction-modification methylase
                     domain-containing protein"
                     /protein_id="HFO9991073.1"
                     /translation="MLQKLDVADSVRRELSPRTARKHKAEFGQFMTPSGVARFMASLF
                     PPSTMKTCRLLDAGAGVGALSCAFLDRWLVGEFGFESVEATAYEIDKKLCGHLAKHLT
                     GYRRVTPRIIEGDYIELATAEGLQDRGYTHAILNPPYKKINSQSAHRQALRTVGIETV
                     NLYSAFVALAVGEVAPGGQIVAIIPRSFCNGPYYRPFRNFILERAAIRHIHLFESRSK
                     AFRDDDVLQENIIIRLERGGLQEAVTVTTSTDDSFFDLVTYEHPFDQIVYPDDSERFI
                     HVPTTHEKSAIELSSAVQCSLADIGVKVSTGPIVDFRLKAHLRSMPEEGTVPLIYPSH
                     LSMSSTVWPVEGLKKPNAIMRNDETEKWLYPNGFYCVVRRFSSKEEKRRVVASVVDPA
                     TFSEYSVLGFENHMNVFHENKHGLPEALARGLAVFLNTTAVDKYFRRFNGHTQVNATD
                     LKMIKYPSRDTLIELGKWAMQQETLTQEHIDAKLGALTA"
     gene            complement(16496..17209)
                     /locus_tag="ACHKIR_003388"
     CDS             complement(16496..17209)
                     /locus_tag="ACHKIR_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HFO9991074.1"
                     /translation="MNMKKKPLTPEQLEDAKRLKSIFNAKKKELGLSQESLAYELGVT
                     QSAVNQLMAGINAINASHAAQLAKILNVKVGDFSPSLAKSIAEMALAIEEPLTRVPAY
                     EYPLLSCVQAGAFSMDDISYTAKDAIKWISTTTKASDRSFWLEVKGHSMTAPQGGKPS
                     FPEGMLILVDPEREIEDGDFCVARMNGDEFTFKRFIRESGKAYLEPLNPRFDMIECNE
                     NCQFVGKVIKSQWNDETFD"
     gene            17305..17508
                     /locus_tag="ACHKIR_003389"
     CDS             17305..17508
                     /locus_tag="ACHKIR_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001240876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFO9991075.1"
                     /translation="MAMKKETLADYVYRFGQKKAAKDFGVAQSAISKALLVGREIYVK
                     TFDDGRVEAEEVRPFPAFVRGDD"
     gene            17563..17832
                     /locus_tag="ACHKIR_003390"
     CDS             17563..17832
                     /locus_tag="ACHKIR_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001115081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9991076.1"
                     /translation="MPEQYCDADRDWIQEQLLQLDPTTRVKIAAEYARVYQEEWDKEP
                     VSFRKGNRARRSANTRLRVYVQKYARASRGWMLSPVAVRKFPPTP"
     gene            18040..18417
                     /locus_tag="ACHKIR_003391"
     CDS             18040..18417
                     /locus_tag="ACHKIR_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9991077.1"
                     /translation="MSTATKSTLIRTLSTVPLRTEERYSFLADVVTLLESQGMHVASN
                     VTVRIDGRNFRVDILATAKTGGSVAIEIDRSSPRSRSVMKLRELARRGTEGFVLLRMP
                     KKLTSYSDAGIDIIPANGKGASC"
     gene            18411..19931
                     /locus_tag="ACHKIR_003392"
     CDS             18411..19931
                     /locus_tag="ACHKIR_003392"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFO9991078.1"
                     /translation="MLKITPNFAQERGLNQLRHQWKQHRTYLMYAPTGSGKTGLAAFV
                     TAGMVERGMRVMFVCPYTILLNQTAERFTEYGLPWEEISFVWRDHPNYDPSRLIQIAS
                     ADTLIRREFPDNIDLLIIDEAHMKRRALLEVIRDRDIRVLGLSGTPFAAWMGKYYECL
                     IKPTTIRELIQRGDLSDYEFFAPSMPDLAGVKTSNTVFGRDYNEEQLASIMGSSDLVG
                     DIVRNWLENGEDLPTICFCVNVAHANFVTREFLQAGIGAEVMTADTLHDERQDIIRRF
                     EEGATKIIVNVGVLVAGFDSDVRCLIYARPTKSEIRWLQCIGRALRTAPGKKRALIFD
                     HSGTVHRLGFPEDIEYDELPGKNDGMKASAGGSEVKAEKLPRECSKCHFMKPAGVHMC
                     PKCGFRPLGGDDVATDRDRKLSRVNKGKREYSREEKQRWWSEIKGYQNYRNATGKPLS
                     DGWCAHTYKEKFGVWPKGFSNAPLQTSVEVHNFIRSKIIAYAKGRKKAMTGGQHAD"
     gene            19921..20892
                     /locus_tag="ACHKIR_003393"
     CDS             19921..20892
                     /locus_tag="ACHKIR_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001694414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primase-helicase zinc-binding domain-containing
                     protein"
                     /protein_id="HFO9991079.1"
                     /translation="MQTKKAAVGQWGRIFEYYGMPPVTGVKHYNGPCPICGARGKFRC
                     DDKDGSGSWICVCGHGDGMKLLQLATGKPWVTLCDEIDRLIGNTWKREKVSQPVTEIS
                     RKRELVMDKFAGLPCLRGTTGEAYLQGRGILQLPTESVRFCDRQIASGREYQAIYAIA
                     TDDRGSLCYLHRTLLDGDRKANVEAAKKMTALQELPGLQHAKSVAIRLYPVSSTLGIA
                     EGIETALSCRQIFRCNVWSTMNSGFMEKFIAPPGVNHLIIFADNDEHGAGLAAAFKCG
                     HKNLMSRNDVEKVSIRWPDLPDFNDMLIQGCEAREHVLTRKFKAEAA"
     gene            20892..21341
                     /locus_tag="ACHKIR_003394"
     CDS             20892..21341
                     /locus_tag="ACHKIR_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HFO9991080.1"
                     /translation="MEIEMLKAANGVFVPAYERDLPRLAKFKNGELYTLEAKLTRNPS
                     FHRKMFAFLNFCFQYWCAEHAGYEFSDEATQFDEFRKNLTILAGFYDVVTTIRGEVRY
                     RAKSLSYANMDQDEFERCYNAMINAALKHVFGCSNNPELNNRLLSFF"
     gene            21349..21912
                     /locus_tag="ACHKIR_003395"
     CDS             21349..21912
                     /locus_tag="ACHKIR_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HFO9991081.1"
                     /translation="MKQRKPKKCKVCGSSFVPFRSYQKVCCGQCALELVRKEKAIASA
                     KEQADKLKARRRDLQPRSYWIKQAQQAVNAYIRERDRHLPCVSCGTFDSAQWDAGHYR
                     TTAAAPQLRFDERNIHKQCVVCNQYKSGNLVPYRVELINRIGQEAVDEIESNHSRHRW
                     TVEECKAIKAEYQQKLKDLRNSRSEAA"
     gene            21909..22103
                     /locus_tag="ACHKIR_003396"
     CDS             21909..22103
                     /locus_tag="ACHKIR_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage NinH family protein"
                     /protein_id="HFO9991082.1"
                     /translation="MTFTVKTIPDMLVEAYGNQTEVARILNCNRATVRKYIGDKEGKR
                     HAIVNGVLMVHRGWGKDTDA"
     gene            22096..22530
                     /locus_tag="ACHKIR_003397"
     CDS             22096..22530
                     /locus_tag="ACHKIR_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFO9991083.1"
                     /translation="MRDIRQVLERWGAWAANNHEDVTWSPIAAGFKRLIPEKVKSRPQ
                     CCDDDAMIICGCMARLNRNNSDLHDLLVDYYVLGETFMALARKHGCSDTCIGKRLHKA
                     EGIVEGMLMMLGVRLEMDRYVERELPGGRTSVFYQRKNSLRS"
     gene            23037..23984
                     /gene="stxA1a"
                     /locus_tag="ACHKIR_003398"
     CDS             23037..23984
                     /gene="stxA1a"
                     /locus_tag="ACHKIR_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1a subunit A"
                     /protein_id="HFO9991084.1"
                     /translation="MKIIIFRVLTFFFVIFSVNVVAKEFTLDFSTAKTYVDSLNVIRS
                     AIGTPLQTISSGGTSLLMIDSGTGDNLFAVDVRGIDPEEGRFNNLRLIVERNNLYVTG
                     FVNRTNNVFYRFADFSHVTFPGTTAVTLSGDSSYTTLQRVAGISRTGMQINRHSLTTS
                     YLDLMSHSGTSLTQSVARAMLRFVTVTAEALRFRQIQRGFRTTLDDLSGRSYVMTAED
                     VDLTLNWGRLSSVLPDYHGQDSVRVGRISFGSINAILGSVALILNCHHHASRVARMAS
                     DEFPSMCPADGRVRGITHNKILWDSSTLGAILMRRTISS"
     gene            23994..24263
                     /gene="stxB1a"
                     /locus_tag="ACHKIR_003399"
     CDS             23994..24263
                     /gene="stxB1a"
                     /locus_tag="ACHKIR_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1a subunit B"
                     /protein_id="HFO9991085.1"
                     /translation="MKKTLLIAASLSFFSASALATPDCVTGKVEYTKYNDDDTFTVKV
                     GDKELFTNRWNLQSLLLSAQITGMTVTIKTNACHNGGGFSEVIFR"
     gene            24767..26734
                     /locus_tag="ACHKIR_003400"
     CDS             24767..26734
                     /locus_tag="ACHKIR_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFO9991086.1"
                     /translation="MAFKHYDVVRAASPSDLAERITQKLKEGWQPYGSALISTAGYGA
                     EFIQPVVSEGELPSLAESGNHPHVSAKPEAAPEYYYVIALAGQSNSMSYGEGLPLPET
                     YDRPDPRIKQLARRSTVTPGGAACKYNDIIPADHCLHDVQDMSRLNHPKADLSKGQYG
                     TVGQGLHIAKKLLPFIPANAGILLVPCCRGGSAFTTGADGTYSDASGASENSTRWGVD
                     KPLYKDLIGRTKAALKKNPKNVLFAVVWMQGEFDFGGTPVNHAAQFGALVDKFRADLA
                     DMAGQCVGGSAGGVPWICGDTTYFWKQKNESTYQTVYGSYKNKTEKNIHFVPFMTDEN
                     GVNVPTNKPEEDPDIPGIGYYGSKWRDSSATWTSQDRASHFSTWARRGIISDRLATAI
                     LVHAGRTAEFITGKQPDMVRPTVPSGEGPEREAEAPASKRTLMSLLASGEDLASQGWR
                     YYHKPAGGDSVNKNIAEAVVSDAGATGGKALQLNKPENHIWFLEHDAAGQGAELLKKG
                     GRVSVRFKLPGSLVPNQFALGIYWQLSSLPEGVMLSGEGNDMLMSFFLQTDTTNLNAM
                     HHRKPNAKLDTFGVFDNGWHTLAFEFAGNNSIQVTPVLDEKRGAAFTLVKSPASGAAD
                     KLQLTDISKSATYTLLIDSIAVEVNSTDTAA"
     gene            26869..27048
                     /locus_tag="ACHKIR_003401"
     CDS             26869..27048
                     /locus_tag="ACHKIR_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFO9991087.1"
                     /translation="MTFIHQVMLYFCTAVCVLYLLSGGYRAMRDFWRRQIDKRAAEKI
                     SASQSAGSKPEEPLI"
     gene            27089..27334
                     /locus_tag="ACHKIR_003402"
     CDS             27089..27334
                     /locus_tag="ACHKIR_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFO9991088.1"
                     /translation="MSEITSLVTAEAVKDVLRSEEVLSALKQKLRHNLEARLDAEVDA
                     ILDELLGAPAAPEPEGIAGEGSASDSGDPTPDSDMMM"
     gene            27412..27627
                     /locus_tag="ACHKIR_003403"
     CDS             27412..27627
                     /locus_tag="ACHKIR_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO9991089.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            27632..>27792
                     /locus_tag="ACHKIR_003404"
     CDS             27632..>27792
                     /locus_tag="ACHKIR_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO9991090.1"
                     /translation="MSRKLRYGLSAVVLALIAAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRG"
BASE COUNT         6822 a         6463 c         7041 g         7466 t
ORIGIN      
        1 gcgatatttg ttttgtttcc aacgccccct gcaattgctt gccagatgcc gttttttacc
       61 gttacggccc cctggttttt taacttccac agcgtatcaa tgacgtcatt caaatcaata
      121 ttcagacctt tagaaatact ttcagctgat gcttttccta atttgttcaa ttctgtaagt
      181 actatatcca tttttttctc ctgatatttt gatgtcggtg gtgctgttat tcggttggag
      241 aggtgtcacc ttcttctacc agcttttcca gttcatccag ctttcgggaa agaatttcac
      301 caaacagatg aagttctgca tctgctgtaa tcgggattgg aacaaatcgt attccacttc
      361 tggcaagttg atttgcgatt tctagacact gccttaattc aactggtgat gcctttgtca
      421 acgtcgtttt ttcgctcatt ggttttctcc agaaatttaa caatacccgg aaccatctca
      481 atggatggtg tgccgcattg gttaccccat acatcaaatc catgcgaggt gtggcgggcg
      541 aacagttcta tccggggaac atcgccaagc agttgcacaa gcttttctcg agccatatcc
      601 ggcttgcggg agtgatcaag gcgtggtgca gtaaatgact ggattattcc ggcgttaatg
      661 cgttcgggca gatttccttt cactgcaaaa aggcagtctt cgctgttagc gcgagtggtg
      721 ctacccatac ccatgaccag cttgtcggtc tgtcttttcc cgcatttgtt ccaggttaat
      781 cctttcatcg tcaccagacg aaagccccac gcttctacaa ccttcaatgc ttcaagtggt
      841 tgtgtaggca cccaccacat agccaacagg caattatcgg ctgccagttc ccataccggg
      901 aggcggcaga tatccagaag actcatgacc ggatatttaa aacttgcacc gcgttgacca
      961 tcggttgctt tgtcgcggaa tgtccagggg ggatctgcgt aaatgagggt gtattttttc
     1021 gtcatagcgc gttttcaaac tcatcaatgt aaagtcctgc tttaatcagt cggcgacggc
     1081 gttcggcctt ttgcacgtat tcctcgcgac agttttttgc tgcgtgttca cggcttctgc
     1141 gactgaatgg aggaacggca ttgcgtgagc gaggtgtgtt acgacgagga tttgattcga
     1201 gggagaaaaa gcgatcaacg attccggtgt ctgtggtgaa tggttctgaa gcctttattt
     1261 tcacaacctt actgcccttt gtcaggccgc gagctacctt gttgaactcg caaagtgaaa
     1321 caccaaattt ctctgctatt tcactgccag taacaggacg accacgagac tgaatcatcc
     1381 aggtgacgcg ttcttttaac ccacggaagg caccgggcct tccagaacgc ctgtagaaag
     1441 cgatctgttt caaatttttg tctccagata tgaaaacccc gacgggatgt cggggatggt
     1501 aggggaagtt acaggcttaa cgagaagcct tttcgggaat ttcgatctga cagtcttgaa
     1561 atatcctgta gcgtgcggcg catttcttcc gtcagatgtt tgaaggcgtt aaattcggcg
     1621 acagcggcgt caacgttata gcccttgctg tgtagctcgt tgaggatgcg catagttggg
     1681 cttggcatat cgaagagcac agaaacgtcc aggttaagag tttttcgatc aacatagctc
     1741 atcatggagt atgggtgatg ctctgaaaac cacgatagag ggtagttgat agatagtgtt
     1801 gattctggaa gctgaagttg ttctgggatt ttttgactga aatagcagtc ttccagtttt
     1861 tcgaacactt cccatgcctg atcggtttcg agcatttttg catgacgggc agcgccgcgt
     1921 tctgtccaga ggatgaggtg ctttgtacgc ggtgcaacta agttactttg agtaaccttg
     1981 ttcttaaatt cccgcaatcc agccccttcc aatttgaagt agtgtttccc acaaacaaaa
     2041 cgctcggcat tgcgtgtata gttcactttg atgttattag tttcggtgcc ataaagttgt
     2101 gccaaaagct cggtggtaat aacaggaatc tggttatggg taatcgggga gatggtttca
     2161 acagagattt gagttgtcat aatgacgccc tctagtggtt tctaaactat caccaccgta
     2221 aggttccaat catcgggtgg tgagacgcac agggttggaa ctaccgggaa accgaccggc
     2281 gagcttttca gctcccccat gcgccccacc ataattcaga tgtgcgcgtg catacgacaa
     2341 taaaaaacac gctcgcggcg tgtgtttgtc gcggtctcta tccggggttc caatcccgac
     2401 gccagatttt gctggcgtgc gaagaatata atcccggata tgtgttgtcg tcaacaagtg
     2461 gcgtgctatc atcgaatagt gttctatcct actcagtgag gtttaacatg cgtacaaccc
     2521 aacaattcag cattacatta accaacgaaa tggctgacat ggtgcgcgcc cgtgtggctt
     2581 ccggtgcata cgcttcagaa agcgaagtta ttcgtgaagg gcttcgtgca ctgaatgaac
     2641 gcgacaaagc aatggaagcg tggttaatac actcagccgc gccgccactc gacgctattc
     2701 gtgagaacct aggcaaaggg cactccattt cacaggttcg ctctgagatt cgcgcccgga
     2761 agtaatctgc atggcatatg aagtgccaca ctaacaaact gctctaggta tgggaatagt
     2821 gtcacttgca gcacctatta gagatccgat aatgaacatc atagcaaggt tcttttattt
     2881 gataattttc tgcatggcaa catcaggatg cactaccact acgaaaataa atcgcggaga
     2941 tgaaaaggaa cagtacatca ttgcctgtgg tgccgctaca ccatggggag tatgctatga
     3001 taaagccaat gccctatgta aaaatggata taaagacatt ttaaaggaac agggatttaa
     3061 caggaaagaa ttaaccattg agtgtaaata aggccatcat ttgcaacgtt gaaaaaattc
     3121 gcccgtgcta cagggaaaaa actccagagc cgcttcgttt aactacgagg attaccacta
     3181 atggcgatgt ttaacccccc tcatcccgga gaaattattg ctgatattct ggaagatcag
     3241 aatattggaa tcagggaact ggcaagagcg cttgatgtcg cgccttccac tgttcagcga
     3301 ctggtttcag gtaatacgac aatatctcct gaaatggctg ttcgtctcgc tgctgtcctg
     3361 ggtggaactc cgtcttcatg gattcgtctc cagacggcat ggagccttga aaaagcggaa
     3421 agagaagttg acgtatctca tctctcaaca aaataccgcc cggcagaaat ttcgcctcat
     3481 gcttaaccac cgcgccgtca ttctggcggc gtcggagagt ggaggatatc aggttccgaa
     3541 ctctgctttg ttctccgcgt ccaggatttc ttcaatcttc tgtactcccc gattattgta
     3601 tcggaagctt tctacctgct tatccgaata cggggatttg tcgatgaacc atttcccgta
     3661 ttgttcggtt ttgagcatgt aggtattggc aatgcgacca accttgttag cggatatttt
     3721 gagttttgct ccaacttctg acgccgaata gtaatgttca ttgatcatcg gtagcgggat
     3781 tacgttagcg cctacaacgg gattaaccag gctggcggca acgacttgtt tagcttcagg
     3841 ggcaaggttg gggaggaagc cgaaaaggtc tttcattgta tcgacggtca ttttcagcgc
     3901 ccgtgcttta cggaattcct caaggccgtt agtcgaatgt ttcgaggtga ttttttgttg
     3961 cagttgctgt tgcattgact ccagttgatc gaccagcgaa cgacggacgg ccttcgactc
     4021 acgcgcggct actcgcaggg cttgcttgaa agtcattgtt ataacttcta tttctgcgcc
     4081 gtttttctga cctacgaaaa tttcgtaggt ctctccttca agctcatcct taactctggc
     4141 aatgaaatca ttattgcgaa cgggcttttc actacataat ttccgtgcct cattgaccat
     4201 tttcaacagc atctggctgt cgattgttgc agccggaaac gaagactgat ttgcaatatt
     4261 gttaatcatg tattctgtcc ttttcagata aaagaaatcc ccgcgagtgc gaggatgtca
     4321 atcactgccg atattcacct ttattgcgaa cacctttacc ggtttatctc cgaagtgtgt
     4381 atgtgtgatt gtcttgattt tatatccgtc atacggggtg tctattctgc ggctggaatc
     4441 gtcgcgcttc ggatatccct ttgtgataat caggcggtca tacttacggt taacgaggcg
     4501 atttatccag tagtcattac acaggcgata ctcttccgtt ttctctccgc gaatcatggc
     4561 atcgaagtat tcacctttga cggcaagttg caggttagcc acggttaacc tcctgcggcg
     4621 gttctggtag cggcatccag tgagttgctt gctcaatacc attacccggc ttaatcgttg
     4681 catctccgcg ccgaaaggtg cttccggtat agcgtgcgga gcatattagc ggttcaacca
     4741 gagagctatc gaaattcacc gaaataagca cgttctggcc cttttcaggc attcgctcac
     4801 tacagcttat ccaaccatcc ggagttaccg gcactggctt ggcggtataa agcggtgtta
     4861 tatctgcccg aaaattacat gctttatgca gccgcaccca ccgttcgact tctgctttgt
     4921 cagaatacat accagtgaac gtgttatatt cacggtcaat ttgcgtgaat gttaccttcc
     4981 acgccaccgg ctctgcttcc agcgatgtca gtgcaatccg cgccagttct tccgcttctt
     5041 ctgctggcag tacaacgttg ctacccggtc cgtatgtttc gcgccactgc ttgattgtaa
     5101 gtagtcgctc tttggtaata gtggtcatac cgtagcccct tcttgatatt tttcaaacca
     5161 gaacacaacc gggtcagatt tcatttcaac caatcccata cgaaccagcg ccttgccttt
     5221 cccggacgca aggaattcac gacgaccatc actgataatt cgccgataat cttccaggct
     5281 actgcaatgc ttgtgcagat tgcatgggtg gcatgccgga accatgttgg atatatcgtc
     5341 acgttcctgg tgaagcatat ttccatcaaa acgaatgacc ggttttacat ggtctgcatg
     5401 ccacttttcg ccaagttcac agccgcaata agcacagcga ccgccaaact tcatgcgcaa
     5461 ttctgcacgt tgttttttcg tcagtgccat ttcactctcc tttgatgcga atgccagcgg
     5521 agcgctcggc ttcactttgt tcccaaaacc acttgtgaag cgccataagc ttttcgtcaa
     5581 tcggtgcata tttgcgatta aagtaggcct gagcatcttt ctcagatttg tccggtaatt
     5641 cgccagggcc aaacagtgtg ttataaatcc atgccagtcc gctcttagcg tcgccagttg
     5701 cctgccattc gataatggca gcctgcatga ccagaatgtt tttcccgatt aacaggtcca
     5761 gttctttgta ccggttgcgg atgtatgcat tctctctttg taattttgcg ttgcgcttct
     5821 ctgcggcttc cagcgccgca accaattcgt ctacagttcc ggcagcttgc agtgcgtaat
     5881 cggtaatagc catctcatgc tcaatttcag taccgttctc attcgttgag gtgatagcaa
     5941 aataatcaga gtcgatttcg ttatcagcta agtggcgtag cgtatcggca acaagctggc
     6001 cgttttcgat tagcagcctc cctaccgtta gcgcaatttc ctcgttctcc tggtcgcggc
     6061 gtttgatgta ttgctggttt ctttcccgtt catccagcag cgccaagacg gtagccggat
     6121 tggctgcggc gatgaattca gcattggcct gctgttccat ttggaaatct tcatcgaaac
     6181 cgctttcagg atgcgctcct tcaattctgc aaatgggaat atatccagca gcctcgcgat
     6241 gaattagtgc atcatcacca tcaaatcggc cctctccata ttcgagcgac cactcgccac
     6301 acgttgcttt ttctgccttt tcacgcagtg cctgatagtc aatctcgctc attggttgcc
     6361 ctccttcata aaaataatcc agtgggtctt gtcacccttt cctgttcgtt gaccgataac
     6421 aggctttctg tcggtcagtg ccaatatttg gcgaacaggt atttgcgttt cattccattt
     6481 aaaaatcaga acgccgtatg gccacaacac acgaaaggct tctttaaatc cctgccgcaa
     6541 atcatcacgc caggtatctt tattcagccg tccatatttc tttcccatcc aggcgttatc
     6601 accaacacgc tcaagatgcg gagggtcgaa tacaacaacc ggaaacgatg cgtctgcaaa
     6661 tggtaatgca cgaaaatctg ctatcaggtc agggctaatt atcagccgtc gtccatcaca
     6721 caatgtgtgc tcttcctttc tgatatcgct aaatatcgcc cggtcgtcat tcttatcgaa
     6781 ccagaacatg cgactgccac agcacatgtc gaggattgca gcatgtccag tcactggttg
     6841 cctcctttgc gaagccgttc cgcccattct tcaagggatt tctccgcata ttcaccggac
     6901 aggccatcaa tcgggtacgg ttcattagcc aactcttctt tcgctgacag aatcatgcgc
     6961 gtaacgtcga aaacttcacg caaagactta ttgataaatc cgtgattgaa cgcagcagca
     7021 agacggctgg cggtatagtt aatcccctcg ttgcgtgctt ccgcacgaat ttcagccaga
     7081 aaagcatcgg tggctggggt ttccgtgaag ttgtcctccc aaccgtagta ctcctgacga
     7141 cagaagttat taaattcctt ctccgactgt ttaagcgccg cattctccgc tgccagctcc
     7201 gaaaacttct cgtgtgccaa cttaacagcc gaatcagcct gcttaattga ctcaatcgct
     7261 ttctgttggt cttcggccag cttcgctaaa ttagcctcca gttcggctat gcgttcgttt
     7321 aatgcatctc gttcatccag tagtgacagc acggtttctg gcccggccat aattttaaag
     7381 gcgtgaatcg catcaatatc aataccaagc tccttgagct tcttttcgct taataaatca
     7441 tcatcacctg gtaatattga cagatgtgtc atcgctgtaa ttgcgcgttc cgacacgtca
     7501 cgcaatacct gatagtcaat ttcggttgtc atattttcgt ctcgttatgg gaggggaatt
     7561 ggttgtaagc tggaattgcc ggatgtttac ccgtgtccgg ggtacgacct cacgttgcag
     7621 cgtacgagaa tggactattg ctcagtgtcg tttgatgtcg gctgaggata ttcgccttgc
     7681 tcttgtaaca gagttagaag aatggaaaac ccctcacgat gtgatgattg atgagtgtcg
     7741 atcccggaat gattgatgtt gttaaattcc aacatcgtgc tcctggcgat agcctcaacg
     7801 gtattccaga atactaatac atctgttttc tgctctgttt tatcagcaac aggaagtttg
     7861 atattttgtt gaaatttatt aatgcaacca ttttgaatat gttcctcgtg cttttcattg
     7921 tcataagcat acagcccgac aaaaacatct cgcacgttac gggatatatt ttccagattc
     7981 gcctttggtt cctttgttaa atcaagcacc gataaatggg ctttaagcgt atccgcagct
     8041 attttctgaa tatcctctgg taaatcttta aattgcatag tataccctcc aagaactgtg
     8101 tattttctcg tgaaatatga gcgatatgtc gtgacgtccc tgtcacagat ttcatcacac
     8161 tgagaacgtg ccgatgaagg ttaagatatc ttcttcttcg aaacgctctt tcagcaggtc
     8221 acggaactct ttggcaattt cttcttccgc agcttcaaca cgcacgatac gtagcaccag
     8281 aacaggctgt tcgcttgtca gaatgcttaa tcgcagcgtg aattccctgt tgcccaggcc
     8341 ctcgtatggg atgcattcaa agcggaaagt ggcaggcatg atttctttgc ttttagcctc
     8401 cacggactcc atgactgagc ggctaccgct gaagttttgc tcttcatatt cagcactgcg
     8461 ttttgcatcg atggtaagac gacggacagc agagatagcc tgtttgatat ccagcacatt
     8521 accgtcgctg tcataggctg tgaggaagtc ggcccagtct tccagccatt cagcgagaga
     8581 cttctggagt tgtcttttgc cgtccacgtc acgtaatgcg cgatatggtg atgtggcttt
     8641 cagttccagc agtgccttgt tgtcagcgtg tccaggttgc tccagagtgc cgatattgaa
     8701 cacagtgact gctttcatcg tttccgcgtt aatgaagcag cgggagcact catcggcata
     8761 ccctttgctg tattcaacaa agtcatcaat actggttgtg gtcatgactc cacggaaacg
     8821 aaaacgttct gtcatgaatc gttccagact ttcgatattt acattgtccg gcaataacgc
     8881 aacagggcat tccgtaagcg gcaaattttc accagaaaaa gcagtagtgg tgagttttac
     8941 aatttcttta atggcattgc tatctaactg agacatgtat atttaccttt aatttatgta
     9001 tttagggata atgcgcgagc gttttaaggc ggggaatatt cagcgtaaaa cagcgtcatg
     9061 ttggccctgt ttatcaaaaa gctgtccctg gtctttctgg aacaaagtaa gctcaccacc
     9121 tttatttaca aacatcgggg tgttagttgt gtcttcttct gatttactac cacgcattgt
     9181 cgggcgtgtg aattttagtt tatgggaaac tgatacgcga ttttcatcaa gtgaagataa
     9241 ttcgaattca acacatacct ttcctttttt gtttgtgctg tttacgccaa aggcaacttc
     9301 acttaatacc gcgccaagtt tgttaacaaa taccccgccg tcgaggtcgt taatgaaaac
     9361 attaacatca gtttgatgct ggttcatatt aatatctccg tgtagaggtg tgttaaggct
     9421 tcagttgttc actgcattta aagcctggtt ttgtttgtga attaaaaact atcgctgccg
     9481 tactgccagt aacctggggc gtccgtgtga ttcgggttta cgtttttgct gcgattgtct
     9541 ttgtctttga gagtggaaac catatttgct accgtttctg gtttctttcc ttcagctttt
     9601 tctttcagtg tctgccctgt ttctgcaatg aatggaatgc ggatttccat ctggtaaccg
     9661 tctgcgccag tctttctgtt tgtggttaat actttctgga ggacgagtcc gattttcttt
     9721 ccgtgaaact caggtgcaac atatttactg acggaaacca tatgttgcgt taactgccca
     9781 attcctgtgc atcccatcat ggcgtgaata atgctcgccc caaatttatt ttcagtaccg
     9841 tcattcttct gaacacaaac actgagatat tggattttcc gtccgtcgtc ggattctcca
     9901 gaaaactcaa tgaatcgggc acctttttct gattgcttga gttctgcttc ggtgatgtta
     9961 atgatatgcg cacccgtttc ggtaataaaa ctaccttgtc cggcggtcag tgctgattct
    10021 tcgttataag taaaaatcac ttcatccatt agttattaac cccccattgt tctccaaata
    10081 caaaaccaat ttccgccagt gcttcgtcca ttttgtcgat gaactccggc accatctcat
    10141 caaaactcgc catgtacttt tcatcccgct cgaccacgac ataatgcagg ccttcacgct
    10201 tcatgcgtgg gtcgtagttg gcgaagtacc aggcgtcctt gtctgtaacc cacatgttga
    10261 attgcacctg ggccatgtaa gcaggcttga tagcgtcaaa gccgccaagc cggaatttca
    10321 tgaagtcgcg ggaagtaaaa gggcatttaa gctcaagacc gttaccgtcg ctgcataaac
    10381 cgtcgggaga gcaggcggtg cgcatgcttt cgtcacgaaa aattattggc gtttccgaga
    10441 cggtaacatc cgcgataaat tcgaagaggg cacgggcatc atcttcgtat tgctttcccc
    10501 atgcgagcga cttagcgtta acttccggtg ccacgccagt acaaacttca gccagcaggg
    10561 tgtggaagta tgacattttt gtatcaggcc atttgctgcc ggaacgtggc tttgctatca
    10621 cattgtgaac ttctgaagca gtgataacac cgagtctcag cttgtgccat ccatcatcgc
    10681 cctggtcgag gctggtaata tccacgccag ttcgctggag aataatttct ggtgtcatga
    10741 cacggcctcg ctgttatttt ccgtggtggc gtgaattttc gcttccgccg tggccttgtt
    10801 tctggcagct ttctttttga caaaatcaag cgttttgaca gcttcttcct gactgagata
    10861 ttcatgtgat gcaatcgtgc gacggaatgt tttggaacat aagggaagca ggtcatccca
    10921 tgtcttgtta atttcggtga ttgcctgagt gatctcattg ataatctcat ctgatgcggg
    10981 agtgacgtca cgctcaggga tgtgatcagc attcaggatg ataccttcac cgccctgagt
    11041 gttcaggtag tcgatagctg tatccaggcg atcgcgacgg ggccagtatt tgctggcgcg
    11101 cttaacaatg gcttttctgg ccatttcatc ggggaaacta tcccacgggc tacttcctcc
    11161 gttatttccg gctttgctgc acgccctgat gacctcgatt tccctgttgc tcatctcttc
    11221 ggtcagatag tcgccttcgg atgtttttac gacacaataa ccgccaatgc gtgcgcccct
    11281 gtctacgaat gggttgtatt tgtgcgtggg ggcgcagtca ataccgttgg actcgtaaat
    11341 gtccttctcg taaacaagtt tgcattgccc ccactgaatg gctcctgtga cctgtgccag
    11401 atgcagaagc cccatataac tgatatccag gcatacagcc ttttttcgtg ggaccagata
    11461 cgccagcttg ctggccgggt tcagggttat gccgatggcg gcaacattga tgatagcgtt
    11521 ctgtgcgctg ggcagatttg cccgtgctgt gtcagccaga aaggcatttt tctggaattg
    11581 ctgaattgca aactgacttt ccttcgccca tgtcagcgtc ggttcagtta atgcctcgca
    11641 gaaaaagcgc tcctgctgct taacaaattc aacgatatcg aacatttttt ggtcctgaaa
    11701 atcagaaagg acagggggag aattttctct cccattcttc ttccgcccga gcataggcga
    11761 tcgctgagat ataatcgttg tacgcctctt cagctttttc gccagtgagt gccagttggg
    11821 cttctttggg taaaaaaagg ctgctcataa gcaatggttt atcggggaac atgctgataa
    11881 gctcctgcgc ccgatcatca atccatttat ccttttcatc ctgaatttgc tgattaatcc
    11941 agcgacgctc ctctatgcgg tcgcaggtga ggtatgcgtt catggcggaa ctcctgattc
    12001 cggttaatgc attaaattaa tttgtcggga aagctgacat acagggcagt tacattcttc
    12061 ctcctgctcc ttagcgaaga aatatgcagc ggcctgtaat gcgatgtctt ctggatgttc
    12121 cgcgataaac ataacattgc cttccgtatc aataacagaa atagcctcat cagacaggat
    12181 gacaaaatag gcgatgattt tatcatccat aaaaacttct cccattatcg ttcctgctgg
    12241 agttacgacg ctttttacat tgatatttat tttttgattg agcatgatat ttcctttcag
    12301 gctggtgaga ttaacggttg gcctttattg ttcaggtaaa cttctattgc atctgagata
    12361 atgcgaattt tttcaatcag tgaatgtgcg taaagtgcat tattaacgtt cgctgacgcc
    12421 atgtaataac gcccgttgta aagaattgct gtgccgggtt taacgtcctc gcgagaaact
    12481 aatgcggttc cgtagtgagg tttgagcatg acaaatcctc cggttaatta atccagatat
    12541 tttatttaat ccccgatatg tgatcgggga tggggttaat taaagattaa cgttgaaacc
    12601 aaagcgggaa gatttttccg atgtgcggga aatatccagc aatttacggc gcatttcttc
    12661 cgtcagatgc ttgaaggcgt taaattcggc gacagcggcg tcaacgttat agcctttgct
    12721 gtgtagctca ttgaggatgc gcatagttgg gcttggcata tcgaagagca cggaagcatc
    12781 aaggctgata accttgcgat caacatagtt catcgtggcg taggggtgat gctctgaaaa
    12841 ccacgacaaa gggaaattga tattcatcgc aggagaagac agggccagtt gtttctgttc
    12901 ccatagttgt ttttccatgc gatcgaattc agcaatgtag gcttccttga atgcagctgc
    12961 ttttttgccg gtgaagccca tcaccagaaa aacgaagccg tttttggtga tttggtacat
    13021 tgggcgttgt tcgcctttgg cgtcggtgta ggtaacgtcc gcaaaattgc gggcgttaaa
    13081 ttccgtagaa caatccatca atcggatttt ttttaaaacg tcgtcgtgcc gcttgtcaaa
    13141 aaattcagca atagcaatag acgttgtgac aacgcgacca ttggtaatgg tgatttcagg
    13201 ttgagaaagg gcagggatag tagccataat ggcagcctcc ttgattggtg attgagaacc
    13261 accgcaggag gtcctaagct cgctggcggt ggactgtaca gggttaggac tactggcaat
    13321 caagggaacc agcccgaccg aagtcggccc catacagccc accattgata agatgtgcgt
    13381 gtatgtcgat acaaaaaaag acgctggcgc gtctgtatcg cctcgattgt cagcggggtc
    13441 ctaatcccga cacccgtttt ataaggtgtt cgccaaatat agccccgaca tcacacgcag
    13501 tcaataccgt cctttctcag aaacgctttg gcggtgccgc ctgcacccca aaacattccc
    13561 tgtatttggt cagcgccaac ttcctgccag tgttgcccgt tctcacgccg ttctcgctct
    13621 cgcgcgggga tactctctca tcgaccggat cgcacccgat gatacagcac gtttacgtgt
    13681 aggggtctaa acaggtcatt gacgctgtaa agctccagat tgttaaagag cattttgcgg
    13741 cgggttaagt cgcgccgtac gactgattta tgtagccctg tgtaagggcg cgatgtttct
    13801 ggcttgaaac aaatataact tgcggtgatt ttcatgtaaa taccgctggt gctttttggg
    13861 gcgaaaaatt attacttaac tgatttttaa agtgatttat tttttaaggg gacagataac
    13921 aggaggggat atgcaaagaa aaacccggcg catgggccgg gctatttatc tggtcctgta
    13981 ctcattcagt agagtagggg ccaagaaatc gctctccgtt gaagtgaata agatgagaag
    14041 gtgcatcagc cacccatacc tctgtttccc atgcgatttc accaagatat cgtcccatga
    14101 tggagcgatt tggaaaagcg gtcacataga caagtccggc tgttgatccg gcaaacagcc
    14161 tggcaagctc agcatgccgc ttcccatcaa ccggtccatg actggtgaca gactcaacca
    14221 gtagcagcca gtttttcgca gtaaaatgta gcaccacatc tggcatttta ccgtgtgaat
    14281 ccacatcaac acctagcccg gccagcagtg gagcgtcgaa gtagccccac ttttcgccag
    14341 tgtcgccagc atagaccagc acgctacccg gagcaaaacg tggggcgaag tcctctatta
    14401 tggcacggat aagttcgctg tgctcgccgg gactgagggt tatttgctgg cccgcagcaa
    14461 tttcaacagg gatacgattc tgttcgcgct ccttagcata gcgggtaacc agcgtttcac
    14521 gctcggctaa ataggttgca aggctatcgt gccatgccgg gctgccgaaa gtgcgtagca
    14581 tggacagagc agcaggttcg atctgataaa cagccttcgg gctgttcact gggcgatcgg
    14641 gcttgtccgg attgtagagg gctactccag cagcgcagaa ctgatgcata gactggcggc
    14701 ggaatgtctc acgagtattg ggtgcgtaga ccttgccgta gtgctcccga acccaattca
    14761 tgattggtgt aatgcccaca agcggatttt ccgcgtcggc ccatgctttt cccggtgtga
    14821 ggttcaggag tgccagcaaa cacaaagcag aacgctcatt ctgctgcgct ctgggcaggc
    14881 ctaaggatgc tataatttgc tgagcggcct caatataatc attttggttg ttcatgcagt
    14941 cagagctcct agcttagcgt caatatgttc ttgcgtgaga gtttcttgtt gcatagccca
    15001 cttgccaagc tctatcagag tgtcacggct tgggtactta atcatcttga ggtcggttgc
    15061 attgacctgt gtatgcccgt tgaagcgacg gaaatactta tccactgcgg tagtgttcag
    15121 gaacacggcc aagccacggg ccagcgcctc gggtaaccca tgtttgttct catgaaacac
    15181 gttcatgtga ttttcaaatc ccaacactga atactcactg aaggtagcag gatcaacgac
    15241 gcttgctacc actcggcgct tttcctcctt cgacgagaaa cgacgcacca cacaataaaa
    15301 accgttcggg taaagccact tttctgtctc gtcgttgcgc atgattgcgt tgggtttctt
    15361 caaaccttcc acaggccaga cagtgctact catactcaaa tggctcgggt agatcagggg
    15421 aacagttcct tcttctggca tgctgcgcaa atgagctttc agtcggaaat caactatcgg
    15481 tccggtcgat accttcacgc caatatcagc cagcgaacat tgtacggcgg atgataactc
    15541 aatggcgctt ttctcgtgcg tggtcggcac atgaataaac cgctccgaat cgtccgggta
    15601 cacaatttgg tcaaatgggt gttcgtaggt gaccaggtca aaaaagctat catcggtcga
    15661 agttgtgact gtaacagcct cctgaaggcc tccacgctcc aggcggatga taatattttc
    15721 ctgcaacaca tcgtcatccc tgaaagcttt gctacgtgat tcgaacaaat gaatatggcg
    15781 gattgctgcc cgctcaagta tgaaattacg gaacggacgg taatacggtc cgttgcagaa
    15841 actacgtggg attatggcca ctatctgccc gccaggtgca acctcaccta cagccagtgc
    15901 aacaaaggcg gaatacagat ttaccgtctc gataccgaca gtgcgaagag cctgccggtg
    15961 ggctgactgg ctattgatct tcttgtaagg cggattaagt attgcatggg tataacctcg
    16021 atcctgtaga ccttcggcgg ttgccagctc aatataatcc ccttcgatga tgcggggcgt
    16081 cactctcctg tacccggtta agtgcttcgc aaggtgacca cacagctttt tgtcgatttc
    16141 gtaggctgtt gcctcgacag actcaaagcc aaactcgcca accagccaac ggtcgaggaa
    16201 ggcgcaagac agtgcgccta caccagcgcc tgcgtccagt agacggcaag ttttcattgt
    16261 gctcggtgga aacaaggagg ccataaaacg ggccacgcca gaaggcgtca tgaactggcc
    16321 gaactcagct ttgtgtttac gcgcggtacg gggagacagc tcgcgacgca cgctgtctgc
    16381 gacgtctagc ttttgaagca taactaatcc tctttacgtg gcatgatgat gccaacgtgc
    16441 ttttgacatc ctccacattc tgcaggcgtg aaggatgtca aatcactctg tgagctcaat
    16501 caaaagtctc atcattccac tgcgatttga tgacctttcc gacaaactgg cagttttcgt
    16561 tacactcaat catgtcgaat cgtgggttga gcggctccag gtacgcttta ccgctctcac
    16621 gaatgaatcg tttgaaggta aattcatcgc cgttcattcg ggccacgcaa aaatcgccat
    16681 cctcgatttc ccgttcaggg tcaacgagta tcagcatccc ttcggggaaa ctgggtttac
    16741 caccctgcgg cgcggtcatt gaatgcccct tgacctccag ccagaacgac ctgtcactgg
    16801 cttttgtggt ggtggagatc catttaatcg catcttttgc ggtgtacgaa atatcatcca
    16861 ttgagaatgc gccagcttgt acgcaggaga gtaacggata ttcgtaagcg gggacacgag
    16921 ttaatggctc ttcaattgcg agcgccattt ctgcaataga ttttgccagg ctgggactga
    16981 aatctccgac ctttacattc agtatcttag caagctgtgc tgcgtggctg gcatttatgg
    17041 cgttgatgcc agccattagc tgattaacag cgctttgggt aacacctaat tcataggcta
    17101 atgattcctg cgacaggcct aactctttct ttttagcgtt aaaaatactt ttcagacgct
    17161 tggcgtcttc cagttgctca ggggttaacg gtttcttttt catgttcata ccatatcacc
    17221 tttggttata tgtaatgaaa tatctgcggt gttgacattt taataactgt gcgtaatgat
    17281 gtgggcattt tgaaggagtc agttatggcg atgaaaaaag agaccctggc tgattacgta
    17341 tacaggttcg gtcagaagaa agccgctaag gactttggtg tggcgcagag cgcgatcagt
    17401 aaagcgcttc tcgtcggacg tgaaatttac gtcaaaacct ttgatgacgg cagagttgag
    17461 gcggaagagg ttcgaccgtt ccctgcgttc gtaagaggcg atgattaatc ctggacacac
    17521 agcccatcgg aggtgtgagt gaacaggcaa atcaaaacgg ttatgcccga gcagtactgt
    17581 gatgctgatc gggactggat acaggagcaa ttgttacagc tagacccgac cacccgggta
    17641 aaaattgcag cagagtacgc gagagtgtac caggaagagt gggacaagga gcctgtatcg
    17701 ttcaggaagg gcaacagggc aagacggagc gccaacaccc ggttacgggt gtatgtccag
    17761 aagtacgcga gagccagccg tggctggatg ctttcgccag tagcggtacg gaagtttccc
    17821 ccgacaccat gatggcagtt agttgcgata gagataattt cacatgcaac taatttcctg
    17881 aaaggaagcg aggagggggt aagggggtgg agtcgttggt aaggcctgta tcccttggcc
    17941 taggccagaa ggcaggtaca taggttaggt agatcactgt ataagcgcgc aatagtttct
    18001 ggtgaaataa ttatcaagct aattattgag tgataaacta tgtcaacagc aacaaaatcg
    18061 acactgatcc gtacactgag cactgtacca ctgagaacag aggagcgtta cagctttctg
    18121 gctgatgttg taacgctcct tgagtcacag gggatgcatg tggcaagcaa tgtcacagta
    18181 agaatcgatg gcagaaattt ccgtgttgat attctcgcaa cagcaaaaac tggtgggagt
    18241 gtggctatcg agatcgatcg tagctctccg cgatcccgct cagtcatgaa attgcgtgaa
    18301 ttggcacgtc gtggcactga aggctttgtg ttgcttcgaa tgccaaagaa actgaccagt
    18361 tatagcgatg ctgggatcga tatcatcccg gcgaatggca agggggcgtc atgctgaaaa
    18421 tcaccccaaa ctttgctcag gagcgcggtt taaaccagct gcggcaccag tggaaacagc
    18481 atcgcacgta cctgatgtac gcacccactg gctctggcaa aactggtctt gcagcattcg
    18541 tcaccgccgg aatggttgag cgtggtatgc gagtgatgtt tgtctgtccg tacaccatcc
    18601 tgctgaacca gacagcggaa cgtttcaccg aatacggttt gccgtgggaa gaaatcagtt
    18661 ttgtatggcg tgatcaccct aactatgacc cgtcacgact gattcagatt gccagtgccg
    18721 acacgctgat ccgtcgtgag tttcctgaca acatcgatct gctgatcatc gatgaagcac
    18781 acatgaagcg tcgtgcgtta cttgaggtta tccgggacag agacatcagg gttcttggac
    18841 tttccggcac cccgttcgca gcctggatgg ggaagtacta cgaatgcctg atcaagccta
    18901 caacgattcg tgaacttatt cagcgtggtg atctgagcga ttacgagttt tttgcccctt
    18961 caatgcctga tctggctggc gtaaaaacca gtaacaccgt atttggacgg gattacaacg
    19021 aggagcagct cgcatccatc atggggagtt cggatctggt tggcgatatc gttcgcaact
    19081 ggcttgagaa tggcgaggat ttgccgacga tctgcttctg cgtgaatgtt gcgcatgcga
    19141 attttgttac ccgtgagttt ttacaggccg gtattggcgc agaggtgatg acggcagata
    19201 ccctccatga tgaacgacag gacatcattc gccgttttga agagggcgcg accaaaataa
    19261 ttgtcaacgt tggtgtactg gttgcgggat ttgacagcga tgttcgctgc ctgatctacg
    19321 cccgtccgac aaaatcagaa atccgttggt tgcagtgcat aggtagagcg ttacgtacag
    19381 ccccaggtaa aaaacgtgcg ttgattttcg accattctgg cacggttcat cggctgggat
    19441 tccctgagga tatcgagtat gacgaacttc cgggaaaaaa tgacggaatg aaagcgtctg
    19501 ctggcgggag tgaggttaag gctgagaaac ttcccagaga atgctccaaa tgccacttca
    19561 tgaagcctgc tggtgtacac atgtgtccca aatgtgggtt tcgtcctctt ggtggcgatg
    19621 atgtggcaac agaccgcgat cgaaaactct cacgcgtaaa taaagggaaa cgcgaataca
    19681 gccgcgagga aaaacagcgg tggtggagtg agatcaaggg gtatcagaat taccgcaacg
    19741 cgacaggtaa gccactgagt gacggatggt gcgctcatac ctacaaagag aagttcgggg
    19801 tgtggcctaa aggcttcagt aacgcgccgc tgcaaacctc ggttgaagtg cacaatttca
    19861 tcaggtcaaa gatcattgcc tatgccaaag ggcgcaagaa agccatgacc ggaggccagc
    19921 atgcagacta agaaggcggc tgtcgggcaa tgggggcgaa tttttgaata ttacggcatg
    19981 cctcctgtta ctggtgtgaa acattacaac ggcccatgcc cgatatgtgg tgccagaggt
    20041 aaatttcgct gtgatgataa agatggctcc ggttcgtgga tttgcgtctg tggtcacggg
    20101 gacgggatga agttgttgca acttgccacg ggtaagccct gggtgacgtt gtgtgatgaa
    20161 atcgatcggc tgatcggaaa tacctggaag agggagaaag tcagccagcc tgtaacagag
    20221 ataagcagaa agcgggagct ggtcatggat aagtttgccg gactcccgtg tctgcggggc
    20281 acaacaggtg aggcgtacct gcaggggagg ggaatactcc agttaccgac cgaatccgtg
    20341 cgtttttgtg accgtcagat cgccagcggg cgcgaatatc aggcaattta cgccattgca
    20401 acagatgaca gaggttctct ttgctatctg catcgtacgt tgctggatgg tgatcgcaag
    20461 gcgaatgtag aggcggctaa aaaaatgacc gcgctacagg agttgcctgg tttgcagcat
    20521 gccaaatcgg tggcaatacg cctgtatccg gtgtcgtcca ctctggggat agccgagggt
    20581 atcgaaactg cgctttcatg ccgtcaaatc ttccgctgca atgtgtggtc aacaatgaac
    20641 tccggtttta tggagaagtt tattgcgcca ccaggcgtta atcacctgat tatctttgca
    20701 gacaatgatg agcacggcgc aggtctggcg gctgccttta aatgtgggca taagaatctc
    20761 atgagtcgta atgacgttga gaaggtcagc attcgctggc ctgacttgcc ggattttaac
    20821 gacatgctca ttcaggggtg tgaagcccgt gaacatgtgt tgacgcgcaa attcaaagcg
    20881 gaggctgcct gatggaaata gagatgctca aggcggctaa tggcgtattt gtaccggcgt
    20941 atgagcgcga tttaccccga ctggcaaaat ttaaaaacgg tgagctgtat acactggaag
    21001 caaaacttac ccgtaaccca tcttttcacc ggaagatgtt cgcttttctt aatttctgct
    21061 ttcagtactg gtgcgctgaa catgctggat acgaattctc tgatgaagcg acgcagtttg
    21121 atgagtttcg taaaaatctg acaattcttg ccgggttcta tgatgtggtc acaaccataa
    21181 gaggcgaggt gagatatcgg gcaaaaagcc tgagttacgc gaatatggat caggatgagt
    21241 ttgaacgttg ctacaacgca atgattaatg ccgcgttaaa acatgtgttc gggtgctcaa
    21301 acaatcctga actgaataac cgcctgctgt cgtttttctg aggtgatgat gaagcaacgt
    21361 aaaccaaaaa aatgcaaagt gtgcggctcc tcgtttgtgc cgttccgctc atatcagaaa
    21421 gtttgctgtg gtcagtgcgc actggaactg gtcagaaaag aaaaggcgat agcttcagca
    21481 aaagagcagg cagacaagct gaaagcgcgc aggagggact tacagccccg cagttactgg
    21541 attaagcagg cacaacaggc ggtgaatgct tatatcaggg agcgggaccg tcatttgccg
    21601 tgtgtttcat gcgggacgtt cgattcagcc cagtgggatg caggccatta ccgtacaaca
    21661 gctgcggcac ctcagctcag atttgatgaa cgcaatatcc ataagcaatg cgtggtgtgt
    21721 aaccagtaca aaagcggaaa tctcgttccg tatcgtgtcg aactgattaa ccgcatcggg
    21781 caggaagcag tagacgaaat cgaatcaaac catagtcgcc accgctggac tgtcgaagag
    21841 tgcaaggcga tcaaggcaga gtatcaacag aaacttaaag acctgcgaaa cagcagaagt
    21901 gaggccgcat gacgttcacc gtaaaaacca ttcctgacat gcttgttgag gcatatggaa
    21961 atcagaccga agtggcccga atactgaact gcaatcgtgc cacagtcaga aaatacattg
    22021 gcgataaaga agggaaaaga cacgctatcg tcaacggtgt tcttatggtt caccgcggat
    22081 ggggtaaaga tactgatgcg tgatatccgg caggttcttg agcgctgggg ggcatgggcg
    22141 gcaaataacc atgaggatgt tacatggtcg cccattgctg ccggatttaa gaggctgatc
    22201 cccgaaaaag taaaatcacg tccacagtgt tgtgacgatg acgcgatgat tatatgcggg
    22261 tgtatggctc gccttaacag gaacaacagc gatctgcatg acttgctggt tgattattac
    22321 gtgttggggg agacgttcat ggcgctggca cggaaacatg ggtgctctga cacctgtata
    22381 ggtaaacgcc ttcacaaagc ggaggggatt gttgaaggca tgctgatgat gctgggagtg
    22441 aggcttgaga tggatcggta tgttgagcgt gaattgccgg gagggagaac ctctgtattt
    22501 tatcagcgaa aaaatagttt acgatcgtaa aaatctgcat atcatgataa gagtggttac
    22561 attgccacgc tgcttaaccc gccgatgcgc gggttttttt gtacccagaa tcctgtgagc
    22621 tatacggaaa gtacacagaa aggaaggtgc gaccacaatt aataacaaaa tcttaaaaat
    22681 cgcacatggc actattagtt ttctaaatat tgtgtatttt ttgtattgca ggatgaccct
    22741 gtaatgaagt ttgcgtaaca gaattttgct ctacgagttt gccagcctcc cccagtggct
    22801 ggctttttta tgtccgtaac atcctgtgta tcaataaatg ttgttgtcta cgtacgtcaa
    22861 gtagtcgcat gagatctgac cagatatgtt aaggttgcag ctctctttga atataattat
    22921 cattttcatt acgttattgt tacgtttatc cggtgcgccg taaaacgccg tccttcaggg
    22981 ggtggaggat gtcaagaata tagttatcgt atggtgctca aggagtattg tgtaatatga
    23041 aaataattat ttttagagtg ctaacttttt tctttgttat cttttcagtt aatgtggttg
    23101 cgaaggaatt taccttagac ttctcgactg caaagacgta tgtagattcg ctgaatgtca
    23161 ttcgctctgc aataggtact ccattacaga ctatttcatc aggaggtacg tctttactga
    23221 tgattgatag tggcacaggg gataatttgt ttgcagttga tgtcagaggg atagatccag
    23281 aggaagggcg gtttaataat ctacggctta ttgttgaacg aaataattta tatgtgacag
    23341 gatttgttaa caggacaaat aatgtttttt atcgctttgc tgatttttca catgttacct
    23401 ttccaggtac aacagcggtt acattgtctg gtgacagtag ctataccacg ttacagcgtg
    23461 ttgcagggat cagtcgtacg gggatgcaga taaatcgcca ttcgttgact acttcttatc
    23521 tggatttaat gtcgcatagt ggaacctcac tgacgcagtc tgtggcaaga gcgatgttac
    23581 ggtttgttac tgtgacagct gaagctttac gttttcggca aatacagagg ggatttcgta
    23641 caacactgga tgatctcagt gggcgttctt atgtaatgac tgctgaagat gttgatctta
    23701 cattgaactg gggaaggttg agtagtgtcc tgcctgacta tcatggacaa gactctgttc
    23761 gtgtaggaag aatttctttt ggaagcatta atgcaattct gggaagcgtg gcattaatac
    23821 tgaattgtca tcatcatgca tcgcgagttg ccagaatggc atctgatgag tttccttcta
    23881 tgtgtccggc agatggaaga gtccgtggga ttacgcacaa taaaatattg tgggattcat
    23941 ccactctggg ggcaattctg atgcgcagaa ctattagcag ttgagggggt aaaatgaaaa
    24001 aaacattatt aatagctgca tcgctttcat ttttttcagc aagtgcgctg gcgacgcctg
    24061 attgtgtaac tggaaaggtg gagtatacaa aatataatga tgacgatacc tttacagtta
    24121 aagtgggtga taaagaatta tttaccaaca gatggaatct tcagtctctt cttctcagtg
    24181 cgcaaattac ggggatgact gtaaccatta aaactaatgc ctgtcataat ggagggggat
    24241 tcagcgaagt tatttttcgt tgactcagaa tagctcagtg aaaatagcag gcggagattc
    24301 ataaatgtta aatacatctc aattcagtca gttgttgcct gtctgataat agatgtgtta
    24361 gaaaatttct gcatggtgaa tccccctgtg cggaggggcg actggtgaac ggtatgatct
    24421 ctttgatgat cgtaagcgag aatacgcggg tttggtggca ccaggccgaa ctcaccggga
    24481 ggcacccggc accatgcagt attcagagat taggcatata tccaggctcc tcatcgcagg
    24541 agccttttta catgcaaaaa aaaacgctcc tgggaagagc ggctggcaag aagaaacaat
    24601 atgaacaatt aattaacgat gggaataata ccttagagta atcaccttgc gcaactgtaa
    24661 gggcatattt catctttgcg ggctgttttt ctgtgtggct tctgtgtttc cggagggcag
    24721 cctgtacctt ttctgactca gaacattatc ccggccggga ggattcatgg catttaaaca
    24781 ctacgatgtg gtcagggcgg catcgccgtc agaccttgct gaacgaataa ctcaaaaact
    24841 gaaggaaggg tggcagcctt atggtagtgc gctgatttcg acagctggtt atggtgcaga
    24901 gtttatccag ccagttgtga gtgaggggga gcttccctca cttgcagagt caggcaatca
    24961 cccgcatgtt tcagcgaagc ctgaagcagc gccggaatat tactatgtga ttgcgcttgc
    25021 cggtcagtcc aatagcatgt catacggtga aggtcttccg ttgccggaga catatgaccg
    25081 tccggacccg cgtattaagc agctggcgcg ccgcagtacg gtgacaccgg gcggtgcagc
    25141 ctgtaagtat aacgacatca ttccggcgga ccattgtctg catgatgtgc aggacatgag
    25201 ccgccttaac catccgaaag cggacctgtc aaaggggcag tacggtaccg tggggcaggg
    25261 gctgcatatc gccaaaaaac tgctgccgtt tataccggcg aatgcgggca ttctgctggt
    25321 tccgtgctgt cgtggtggct ctgcgttcac caccggagct gatggcacat acagtgacgc
    25381 gagtggtgcc tcggagaatt caacccgctg gggtgtggac aagccgctgt ataaggacct
    25441 tatcggtcga acaaaagcag cactgaagaa gaacccgaaa aatgtgctgt ttgccgtggt
    25501 gtggatgcag ggggaatttg attttggcgg tacgccggta aatcatgccg cacagtttgg
    25561 tgcgctggtt gataaattcc gtgcagacct ggcggatatg gcaggtcagt gcgttggtgg
    25621 ctctgctggc ggtgttccct ggatatgcgg ggacacgacg tatttctgga agcagaagaa
    25681 cgaatccacg taccagacgg tgtacggcag ctataaaaac aaaacggaaa agaatatcca
    25741 tttcgtaccg ttcatgacgg atgagaacgg ggtgaatgtg ccgacgaaca aaccggaaga
    25801 agacccggac attccgggta tcgggtatta cggttcgaaa tggcgtgaca gctcagccac
    25861 ctggacgtca caggacaggg cgagccattt tagcacctgg gctcgccggg ggattatttc
    25921 cgaccgtctg gcaacggcga ttctggtgca cgccgggaga accgctgaat tcattaccgg
    25981 aaaacagcct gatatggtga ggcccaccgt accttccggt gaaggtccgg agagagaggc
    26041 ggaagccccg gccagtaagc gtaccctgat gagtctgctg gcatccggcg aagacctggc
    26101 atcacagggc tggcgctatt atcacaaacc ggcgggcgga gacagtgtta acaaaaacat
    26161 tgctgaagcg gtggtcagtg atgcgggggc tacgggaggt aaggctctgc aactgaacaa
    26221 accggaaaac cacatctggt ttctggagca tgatgcagcc ggacaggggg cggagttact
    26281 gaagaagggc ggacgtgtga gcgtacggtt taagttgccg ggttcactgg tgccgaatca
    26341 gtttgccctg ggcatttact ggcagttgtc gtccctgccg gagggggtga tgctgtcagg
    26401 agaaggtaac gatatgctga tgtctttctt cctgcagacg gatacgacga acctgaacgc
    26461 gatgcatcac aggaagccga atgcgaagct ggatacattc ggggtctttg ataacggatg
    26521 gcacacgctg gcttttgagt ttgccggaaa caacagcatt caggtgacac cagtactgga
    26581 tgagaaacga ggagcggcgt tcacactggt gaagtcaccg gcatcggggg cggcggacaa
    26641 actgcaactg accgatatat caaaatcggc gacgtatacg ctgctgattg acagcattgc
    26701 ggtggaagtg aacagcacag acactgcggc atgataaaaa aagccgccag ttaccggaat
    26761 ggactggcgg tggtaatacc catggagaaa tataaaggaa cgatactttc gtgctctgtt
    26821 tttttgaatg aaaacagttc ctattgtcaa caataacggt aagaaattat gacttttatt
    26881 catcaggtga tgctgtactt ctgtacggca gtctgtgtgc tgtatctcct ttcgggtgga
    26941 taccgggcca tgcgtgactt ctggcgcaga cagattgaca aaagggccgc tgagaaaatc
    27001 agcgccagtc agtcagccgg aagcaaaccc gaagagccgc tcatttagcg gcaactttct
    27061 taatcacacc tttcgacgag aaaatcccat gtcagaaatt acatccctgg tcactgctga
    27121 agcagtgaag gacgtcctgc gctctgaaga agtcctgagc gcactgaaac agaaacttcg
    27181 ccataacctg gaagcgcgtc ttgatgcaga agtggatgcc attctggatg aactgctggg
    27241 cgcaccggca gctccggagc cggaaggcat cgcgggtgag gggagtgctt cagatagcgg
    27301 tgaccccaca cctgacagcg acatgatgat gtaagcatgc gtcagggacc atcggtgtgt
    27361 gccggtggtc ttttttattg ttgtgagctt ccggcttgcg ggagacgggg tatgtaccag
    27421 atggaaaaaa tcacaacagg tgtgtcatac accacgtcag cggtgggaac gggctactgg
    27481 ttcctgcagt tgctggacag ggtttccccg tctcagtggg cggcaatagg cgtgctgggg
    27541 agtctgctgt ttgggctgct gacatacctg accaacctgt atttcaaaat cagagaagac
    27601 cgtcgtaagg cggcgcgggg agagtaagct gatgagcagg aaactccgct atggtttatc
    27661 ggctgtcgtt ctggcgctga ttgccgcagg ggcttctgcg cctgaaatcc tcgaccagtt
    27721 tctggatgaa aaggaaggta accacaccac agcataccgt gatggtgcgg gtatctggac
    27781 catctgccgt gg
//
LOCUS       DAZGQL010000073        27587 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.073, whole genome shotgun sequence.
ACCESSION   DAZGQL010000073 DAZGQL010000000
VERSION     DAZGQL010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27587
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.073"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            194..1132
                     /gene="tdcA"
                     /locus_tag="ACHKIR_003405"
     CDS             194..1132
                     /gene="tdcA"
                     /locus_tag="ACHKIR_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HFO9991091.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVD"
     gene            1231..2220
                     /gene="tdcB"
                     /locus_tag="ACHKIR_003406"
     CDS             1231..2220
                     /gene="tdcB"
                     /locus_tag="ACHKIR_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HFO9991092.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            2242..3573
                     /gene="tdcC"
                     /locus_tag="ACHKIR_003407"
     CDS             2242..3573
                     /gene="tdcC"
                     /locus_tag="ACHKIR_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HFO9991093.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTLSMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            3599..4807
                     /gene="tdcD"
                     /locus_tag="ACHKIR_003408"
     CDS             3599..4807
                     /gene="tdcD"
                     /locus_tag="ACHKIR_003408"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HFO9991094.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLNASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLHRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            4841..7135
                     /gene="tdcE"
                     /locus_tag="ACHKIR_003409"
     CDS             4841..7135
                     /gene="tdcE"
                     /locus_tag="ACHKIR_003409"
                     /EC_number="2.3.1.-"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HFO9991095.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPLDFDTNIATTITAHDAGYIDQPLEKIV
                     GLQTDAPLKRALHPFGGINMIRSSFHAYGREMDSEFEYIFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRRALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEALPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGMDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            7149..7538
                     /gene="tdcF"
                     /locus_tag="ACHKIR_003410"
     CDS             7149..7538
                     /gene="tdcF"
                     /locus_tag="ACHKIR_003410"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HFO9991096.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            7610..8974
                     /gene="tdcG"
                     /locus_tag="ACHKIR_003411"
     CDS             7610..8974
                     /gene="tdcG"
                     /locus_tag="ACHKIR_003411"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFO9991097.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEELLSKTYYSVGGGFIVEEEHFGLS
                     HDVETPVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            9249..10580
                     /gene="cyuP"
                     /locus_tag="ACHKIR_003412"
     CDS             9249..10580
                     /gene="cyuP"
                     /locus_tag="ACHKIR_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO9991098.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGVLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            10608..11918
                     /gene="cyuA"
                     /locus_tag="ACHKIR_003413"
     CDS             10608..11918
                     /gene="cyuA"
                     /locus_tag="ACHKIR_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfidase"
                     /protein_id="HFO9991099.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCDEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTQQACVTEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCARGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYEIISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(12050..12214)
                     /gene="yhaL"
                     /locus_tag="ACHKIR_003414"
     CDS             complement(12050..12214)
                     /gene="yhaL"
                     /locus_tag="ACHKIR_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaL"
                     /protein_id="HFO9991100.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(12237..12938)
                     /gene="yhaK"
                     /locus_tag="ACHKIR_003415"
     CDS             complement(12237..12938)
                     /gene="yhaK"
                     /locus_tag="ACHKIR_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HFO9991101.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQQLIASPEGTVGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            13043..13939
                     /gene="yhaJ"
                     /locus_tag="ACHKIR_003416"
     CDS             13043..13939
                     /gene="yhaJ"
                     /locus_tag="ACHKIR_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HFO9991102.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFSGK"
     gene            complement(13990..14346)
                     /locus_tag="ACHKIR_003417"
     CDS             complement(13990..14346)
                     /locus_tag="ACHKIR_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFO9991103.1"
                     /translation="MQWYLAVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLEFPFLSLIYLAATIIPVIALCVRRLHDTDRSGAWALLYLVPIIGWLVLFVFACLEG
                     NSGSNRYGNDPKFGSN"
     gene            complement(14589..14954)
                     /gene="yhaH"
                     /locus_tag="ACHKIR_003418"
     CDS             complement(14589..14954)
                     /gene="yhaH"
                     /locus_tag="ACHKIR_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFO9991104.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
     gene            complement(15245..16231)
                     /gene="yqjG"
                     /locus_tag="ACHKIR_003419"
     CDS             complement(15245..16231)
                     /gene="yqjG"
                     /locus_tag="ACHKIR_003419"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HFO9991105.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFNHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            complement(16301..16693)
                     /gene="yqjF"
                     /locus_tag="ACHKIR_003420"
     CDS             complement(16301..16693)
                     /gene="yqjF"
                     /locus_tag="ACHKIR_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFO9991106.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITGYAGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            complement(16880..17179)
                     /gene="yqjK"
                     /locus_tag="ACHKIR_003421"
     CDS             complement(16880..17179)
                     /gene="yqjK"
                     /locus_tag="ACHKIR_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HFO9991107.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            complement(17169..17573)
                     /gene="yqjE"
                     /locus_tag="ACHKIR_003422"
     CDS             complement(17169..17573)
                     /gene="yqjE"
                     /locus_tag="ACHKIR_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFO9991108.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            complement(17576..17881)
                     /gene="yqjD"
                     /locus_tag="ACHKIR_003423"
     CDS             complement(17576..17881)
                     /gene="yqjD"
                     /locus_tag="ACHKIR_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqjD family protein"
                     /protein_id="HFO9991109.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            complement(17919..18287)
                     /gene="yqjC"
                     /locus_tag="ACHKIR_003424"
     CDS             complement(17919..18287)
                     /gene="yqjC"
                     /locus_tag="ACHKIR_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 family protein YqjC"
                     /protein_id="HFO9991110.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHQKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            complement(18434..18817)
                     /gene="mzrA"
                     /locus_tag="ACHKIR_003425"
     CDS             complement(18434..18817)
                     /gene="mzrA"
                     /locus_tag="ACHKIR_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HFO9991111.1"
                     /translation="MQIPRMSLRQLAWSGAVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            complement(18821..19483)
                     /gene="yqjA"
                     /locus_tag="ACHKIR_003426"
     CDS             complement(18821..19483)
                     /gene="yqjA"
                     /locus_tag="ACHKIR_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YqjA"
                     /protein_id="HFO9991112.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            complement(19505..19588)
                     /locus_tag="ACHKIR_003427"
     CDS             complement(19505..19588)
                     /locus_tag="ACHKIR_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9991113.1"
                     /translation="MLMYQTRRTYQDSNNIAVVHHLKPAWR"
     gene            complement(19828..20604)
                     /gene="exuR"
                     /locus_tag="ACHKIR_003428"
     CDS             complement(19828..20604)
                     /gene="exuR"
                     /locus_tag="ACHKIR_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HFO9991114.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQATDNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDARTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            complement(20734..22032)
                     /gene="exuT"
                     /locus_tag="ACHKIR_003429"
     CDS             complement(20734..22032)
                     /gene="exuT"
                     /locus_tag="ACHKIR_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HFO9991115.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVDTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQESHNDPAPQH"
     gene            22515..23927
                     /gene="uxaC"
                     /locus_tag="ACHKIR_003430"
     CDS             22515..23927
                     /gene="uxaC"
                     /locus_tag="ACHKIR_003430"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFO9991116.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            23942..25429
                     /gene="uxaA"
                     /locus_tag="ACHKIR_003431"
     CDS             23942..25429
                     /gene="uxaA"
                     /locus_tag="ACHKIR_003431"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HFO9991117.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIAHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            25512..26063
                     /gene="ygjV"
                     /locus_tag="ACHKIR_003432"
     CDS             25512..26063
                     /gene="ygjV"
                     /locus_tag="ACHKIR_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HFO9991118.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAIDERG"
     gene            complement(26068..27312)
                     /gene="sstT"
                     /locus_tag="ACHKIR_003433"
     CDS             complement(26068..27312)
                     /gene="sstT"
                     /locus_tag="ACHKIR_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFO9991119.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVIF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLINDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
BASE COUNT         6826 a         6744 c         7203 g         6814 t
ORIGIN      
        1 gtcatttttt ttgacaaaaa tcagggttta tgctgatttt tatactttaa ctcattgata
       61 tataaagata tttaattgta ataacgatac cctggaaagt attgaaagtt aatttgtgag
      121 tggtcgcaca tatcctgttc atttcatttt gatacacttc atgccgtcaa tgaggtaatt
      181 aacgtaggtc gttatgagca ctattcttct tccgaaaacg cagcacctgg tagtctttca
      241 ggaagttatt agaagtggtt ctatcggctc ggctgcaaaa gagttagggt taactcaacc
      301 ggccgtcagt aaaatcatta acgatattga agattatttt ggtgtggagt tagtggtgcg
      361 gaaaaatacc ggtgtaacat taacacctgc cggtcaattg ttactctccc gttccgaatc
      421 cattacccgt gaaatgaaaa atatggttaa tgagataagc ggtatgtctt ctgaggcggt
      481 ggtggaagtc tcatttggtt ttccttcatt gattggtttt acttttatgt cagggatgat
      541 caacaagttc aaagaggtgt tcccgaaagc gcaggtttct atgtatgaag cgcaactgtc
      601 ttcgttctta ccggcaatcc gcgacggtcg actggatttt gcgattggta cgttaagcgc
      661 agaaatgaag cttcaggatt tacatgttga gccgctgttc gagtccgagt ttgtgctggt
      721 agccagtaag tcccgaacat gcaccggcac caccacgctg gagtcgttga agaacgaaca
      781 gtgggtgttg ccacaaacca atatggggta ctacagcgaa ctacttacta cgttacaaag
      841 aaatggcatc agtattgaaa acatcgttaa aaccgactca gtcgtgacaa tttataatct
      901 tgttctcaat gctgatttct taactgtaat tccttgtgat atgacatcac cttttggttc
      961 taatcaattt attactattc cggttgaaga aacattacct gtggcacaat atgccgcggt
     1021 atggtcgaaa aattatcgta ttaaaaaagc agcatcggtt ttggtggaat tagccaaaga
     1081 gtattcatct tataatgggt gtagacgaag gcaattaatt gaagttgatt agttatttgt
     1141 tttaatttaa acataaataa tccacctgtc tgttttgcag gtgtcggtta cggttaccta
     1201 catatttaat tcaggctaag aggttttata atgcatatta catacgatct cccggttgct
     1261 attgatgaca tcattgaagc aaaacaacga ctggctgggc gaatttataa aacaggtatg
     1321 cctcgctcca actattttag tgaacgttgc aaaggtgaaa tattcctcaa attcgaaaac
     1381 atgcagcgta ccggttcatt taaaattcgt ggcgcattta ataaattaag ttcactgact
     1441 gatgcggaaa aacgtaaagg tgtagtggcc tgctctgcgg gcaaccatgc gcaaggggtt
     1501 tcgctctcct gtgcgatgct gggtattgac ggtaaagtgg tgatgccaaa aggtgcgccg
     1561 aaatccaaag tcgcggcaac gtgcgactac tccgcagaag tcgttttgca tggtgataac
     1621 ttcaacgaca ctattgctaa agtgagcgaa attgtcgaaa tggaaggccg tatttttatc
     1681 ccaccttacg atgatccgaa agtgattgct ggccagggaa cgattggtct ggaaattatg
     1741 gaagatctct atgatgtcga taatgtgatt gtgccaattg gcggtggcgg tttaattgct
     1801 ggtattgcgg tagcgattaa atcaattaac ccgactattc gtgttattgg cgtacagtca
     1861 gaaaacgttc acggcatggc ggcttctttc cactccggag aaataactac gcaccgaact
     1921 accggcaccc tggcggatgg ttgtgatgtc tcccgcccgg gtaatttaac ttacgaaatc
     1981 gttcgtgaat tagtcgatga catcgtgctg gtcagcgaag acgaaatcag aaacagtatg
     2041 attgccttaa ttcagcgcaa taaagtcgtc accgaaggcg caggcgctct ggcatgtgct
     2101 gcattattaa gcggtaaatt agaccaatat attcaaaaca gaaaaaccgt cagtattatt
     2161 tccggcggca atatcgatct ttctcgcgtc tctcaaatca ccggtttcgt tgacgcttaa
     2221 ttaattcgtt gaggatagga tatgagtact tcagatagca ttgtatccag ccagacaaaa
     2281 caatcgtcct ggcgtaaatc agataccaca tggacgttag gcttgtttgg tacggcaatc
     2341 ggcgccgggg tgctgttctt ccctatccgc gcaggttttg gcggactgat cccgattctt
     2401 ctgatgttgg tattagcata ccccatcgcg ttttattgcc accgggcact ggcgcgtctg
     2461 tgtctttctg gctctaaccc ttccggcaac attacggaaa cggtggaaga gcattttggt
     2521 aaaactggcg gcgtggttat cacgttcctg tacttcttcg cgatttgccc actgctgtgg
     2581 atttatggcg ttactattac caataccttt atgacgttct gggaaaacca gctcggcttt
     2641 gcaccgctga atcgcggctt tgtggcgctg ttcctgttgc tgctgatggc tttcgtcatc
     2701 tggtttggta aggatctgat ggttaaagtg atgagctacc tggtatggcc gtttatcgcc
     2761 agcctggtgc tgatttctct gtcgctgatc ccttactgga actctgcagt tatcgaccag
     2821 gttgacctcg gttcgctgtc gttaaccggt catgacggta tcctgatcac tgtctggctg
     2881 gggatttcca tcatggtttt ctcctttaac ttctcgccaa tcgtctcttc cttcgtggtt
     2941 tctaaacgtg aagagtatga gaaagacttc ggtcgcgact tcaccgaacg taaatgttcc
     3001 caaatcattt ctcgtgccag catgctgatg gttgcagtgg tgatgttctt tgcctttagc
     3061 tgcctgttta cgctgtctcc ggccaacatg gcggaagcca aagcgcagaa tattccagtg
     3121 ctttcttatc tggctaacca ctttgcgtcc atgaccggta ccaaaacaac gttcgcgatt
     3181 acactggaat atgcggcttc catcatcgca ctcgtggcta tcttcaaatc tttcttcggt
     3241 cactatctgg ggacgctgga aggcttgaat ggcctgattc tgaagttcgg ttataaaggt
     3301 gacaaaacca aagtgtcgct gggtaaactg aatactctca gcatgatctt catcatgggc
     3361 tccacctggg ttgttgccta cgccaacccg aacatcctcg acctgattga agccatgggc
     3421 gcaccgatta tcgcatcact gctatgcctg ttgccgatgt atgccattcg taaagcgccg
     3481 tctctggcga aataccgtgg tcgtctggat aacgtgtttg ttaccgtgat tggtctgctg
     3541 actatcctga acatcgtata caaactgttt taatccgtaa ctcaggatga gaaaagagat
     3601 gaatgaattt ccggttgttt tggttattaa ctgtggttcg tcttcgatta agttttccgt
     3661 actcaatgcc agcgactgtg aagtattaat gtcaggtatt gccgacggta ttaactcgga
     3721 aaatgcattc ttatccgtaa atgggggaga gccagcaccg ctggctcacc acagctacga
     3781 aggtgcattg aaggcaattg catttgaact ggaaaaacgg aatttaaatg acagtgtggc
     3841 cttaattggc caccgcatcg ctcacggcgg cagtattttt accgagtccg ccattattac
     3901 cgatgaagtc attgataata tccgtcgcgt ttctccactg gcacccctgc ataattacgc
     3961 caatttaagt ggtattgaat cggcgcagca attatttccg ggcgtaactc aggtagcggt
     4021 atttgatacc agtttccacc agacgatggc tccggaagct tatttatacg gcctgccgtg
     4081 gaaatattat gaagagttag gtgtacgccg ttatggtttc cacggcacgt cgcaccgcta
     4141 tgtttcccag cgcgcgcatt ctttgctgaa tctggcggaa gatgactccg gcctggttgt
     4201 ggcgcatctt ggcaatggcg cgtcaatctg cgcagttcgc aacggtcaga gtgttgatac
     4261 ctcaatggga atgacgccgc tggaaggctt gatgatgggt acccgcagtg gtgatgtcga
     4321 cttcggggcg atgtcctggg tcgccagcca aaccaaccag agcctgggtg acctggaacg
     4381 cgtcgtgaat aaagagtcgg gattattagg tatttccggc ctttcttcgg atttacgtgt
     4441 tctggaaaaa gcctggcatg aaggtcacga acgcgcgcaa ttggcaatta aaacctttgt
     4501 tcatcgaatt gcccgtcata ttgccggaca cgctgcttca ttacatcgcc tggatggaat
     4561 tatattcacc ggcggaatag gagagaattc aagtttaatt cgtcgtctgg tcatggaaca
     4621 tttagctgta ttaggcgtag agattgatac agaaatgaat aatcgctcta actcttttgg
     4681 agagcgaatt gtttccagtg aaaatgcgcg tgtcatttgc gccgtaattc cgactaacga
     4741 agaaaaaatg attgctttgg atgccattca tttaggcaaa gtgaacgcgc ccgcagaatt
     4801 tgcataattt agttgaagta ttgtagagag attatttttc atgaaggtag atattgatac
     4861 cagcgataag ctgtacgccg acgcatggct tggctttaaa ggtacggact ggaaaaacga
     4921 aattaatgtc cgcgatttta ttcaacataa ctatacaccg tatgaaggtg atgaatcttt
     4981 tctcgccgaa gcgacgcctg ccaccacgga attgtgggaa aaagtgatgg aaggcatccg
     5041 tatcgaaaat gcgactcacg cgccattgga tttcgatacc aatattgcca ccacaattac
     5101 cgctcatgat gcaggatata ttgaccagcc gctggaaaaa atagtgggtc tgcaaacgga
     5161 tgcgccatta aagcgtgcgc tgcatccgtt cggcggcatt aatatgatta gaagttcatt
     5221 ccacgcgtat ggtcgtgaaa tggatagcga atttgaatac atctttaccg atctgcgtaa
     5281 aacccataac cagggcgtat ttgatgttta ctcaccggat atgctgcgct gccgtaaatc
     5341 tggtgtactg accggtttac cagatggtta tggtcgtggg cgtatcatcg gtgactaccg
     5401 ccgcgtggcg ctttacggca tcagttatct ggtacgtgaa cgcgaactgc agtttgccga
     5461 tctccagtcc cgtctggaaa aaggcgaaga tctcgaagct acgatccgtc tgcgtgagga
     5521 gctggcggaa catcgtcgtg cgctgttgca gattcaagaa atggcggcga aatacggttt
     5581 tgacatctcc cgtccggcgc agaatgcaca ggaagcggtg cagtggctct acttcgctta
     5641 cctggcggca gtgaaatcgc aaaatggcgg cgcgatgtcg ctggggcgca cggcatcgtt
     5701 cctcgatatc tacattgagc gcgactttaa agcaggcgta ctcaatgagc agcaggcaca
     5761 ggaactgatc gaccacttca tcatgaagat ccgtatggtg cgcttcttgc gtacgccgga
     5821 atttgattcg ctgttctccg gcgacccgat ctgggcgacg gaagtgatcg gcgggatggg
     5881 gctggacggt cgaaccctgg tgaccaaaaa ctccttccgc tatttacaca ccctgcacac
     5941 tatggggcct gcaccggaac ctaacctgac tattctgtgg tcggaagcct tgccgattgc
     6001 cttcaaaaaa tatgccgcgc aggtgtctat cgtcacatcc tctttgcagt atgaaaacga
     6061 cgatctgatg cgtactgact tcaatagcga cgattacgcg attgcctgtt gcgtcagccc
     6121 aatggtgatt ggtaagcaga tgcagttctt cggtgcgcgc gctaacctgg cgaaaacgct
     6181 actgtacgca atcaacggcg gaatggacga gaagctgaag attcaggttg gcccgaaaac
     6241 ggctccgttg atggatgacg tgctggacta cgacaaagtg atggatagcc tcgaccactt
     6301 catggactgg ctggcggtac agtacatcag cgcgctgaat atcatccact acatgcacga
     6361 caagtacagc tatgaagcct cgctgatggc gctacacgat cgtgatgtct atcgcaccat
     6421 ggcgtgcggc atcgcgggcc tgtcggtggc aacggactcc ctgtctgcca tcaaatatgc
     6481 tcgtgtgaaa ccaatccgtg acgaaaacgg cctggcggtg gatttcgaaa tcgacggcga
     6541 atatccgcag tatggcaaca acgacgagcg cgtagacagc attgcctgcg atctggttga
     6601 acgctttatg aagaaaatta aagctctgcc aacctatcgc aatgctgtgc cgactcaatc
     6661 gattctgact atcacctcta acgtggtgta cggtcagaaa accgggaaca cgccggacgg
     6721 tcgccgcgcc ggaacaccgt tcgcgccggg cgctaacccg atgcacggtc gtgatcgcaa
     6781 aggcgccgtg gcctcattga cgtcggtggc gaaactgccg ttcacctacg ccaaagatgg
     6841 gatctcgtac accttctcca tcgtccctgc ggcgctgggc aaagaagatc cagtacgtaa
     6901 aaccaacctt gtcggcctgc tggatgggta tttccaccac gaagcggatg tcgaaggcgg
     6961 tcaacacctc aacgtcaacg taatgaatcg ggaaatgctg ctggatgcca tcgagcaccc
     7021 ggaaaaatat cctaacctga caatccgtgt ctctggctac gccgtgcgct tcaacgcact
     7081 gacccgtgaa cagcaacagg atgttatttc acgtaccttt acccaggcgc tctgacgccg
     7141 gaggatgtat gaaaaagatt atcgaaacgc aacgtgcccc aggcgcaatc ggcccttatg
     7201 ttcagggcgt tgatttaggc agcatggtct tcacctccgg gcaaattccg gtttgcccac
     7261 agaccggtga gatcccggct gatgtgcaag atcaggcgcg tttaagcctc gaaaacgtca
     7321 aagcgatcgt ggttgctgcc gggctgagtg tgggcgatat catcaagatg accgtgttca
     7381 tcaccgatct gaatgatttt gccaccatca atgaagtcta taagcagttc ttcgatgaac
     7441 atcaggcgac ctacccgacc cggagctgtg tgcaggtcgc gcgtttgccg aaagatgtga
     7501 agttggaaat tgaagccatc gcagtacgta gtgcgtaagg cctcgtgagc gggacggtcg
     7561 caaggtcgtt ccgctccact tcactgaacg gcaatccgag ggtgtggata tgattagtgc
     7621 attcgatatt ttcaaaattg ggattggtcc ctccagttcg catactgtgg ggccaatgaa
     7681 tgccggaaaa agttttattg atcggctgga aagtagcggc ttattaaccg cgacgagcca
     7741 tattgtggtc gatctgtacg ggtcgttgtc actgacgggc aaaggccatg ccacggatgt
     7801 cgccatcatc atgggactgg caggaaacag tccgcaggat gttgtcattg atgagatccc
     7861 tgcatttata gagttagtga cgcgcagcgg gcggttgccg gtggcttctg gcgcgcatat
     7921 tgttgatttt cctgtagcaa agaacattat cttccatccc gaaatgttgc ctcgccatga
     7981 gaacggaatg cggatcactg cctggaaggg acaggaagag ctattaagca aaacctatta
     8041 ctctgtcggc ggcgggttta ttgtcgaaga agaacacttc ggcctgtcgc acgatgtcga
     8101 aacacccgta ccttacgatt tccactcagc aggtgaactg ctgaaaatgt gtgattacaa
     8161 cggcctgtct atatctggcc tgatgatgca caacgagctg gcgctgcgca gtaaagcgga
     8221 aattgacgca gggtttgccc gtatctggca agtgatgcat gacggtattg aacgcgggat
     8281 gaacactgaa ggcgtgctgc ctggtccgct caatgtgccg cgtcgtgccg tagcgctgcg
     8341 tcgtcaactg gtttccagcg ataacatctc taacgatccg atgaatgtca tcgactggat
     8401 caatatgtac gcgctggcgg ttagtgaaga aaacgcagct ggcgggcgcg tggtaacggc
     8461 accgactaac ggtgcgtgcg gcattattcc ggcagtgctg gcttattacg ataagttccg
     8521 tcgtccggta aacgagcggt caattgcccg ctattttctt gcagcggggg ctattggcgc
     8581 gctgtataaa atgaacgcct ccatctctgg tgcggaggtc ggctgtcagg gggagattgg
     8641 cgtggcctgt tcaatggcgg cggcagggtt aactgaactc ctgggcggca gtccggcgca
     8701 ggtatgcaat gcggcggaaa tcgcgatgga gcataacctt gggctgacct gcgatccggt
     8761 tgccggacag gtacaaatcc cgtgcattga acgtaatgcc attaatgccg tgaaagcagt
     8821 aaacgccgcg cggatggcga tgcgccgcac ctcggcaccg cgtgtttcac tcgataaagt
     8881 gatcgagacg atgtatgaaa ccggcaaaga tatgaacgat aaataccgcg aaacgtcacg
     8941 cggaggactg gccattaaag tggtctgtgg ctgacattgc tgtcagcttt catccttggg
     9001 tgcacaaatg tgcacctttt ttttgtgatc tgccgcaaat agagaatgaa tattctcttg
     9061 cgattaattt agtagaaaat tcttatccat ctccgttatt tcaaagtctt gattgtgaag
     9121 attttctctg acctcgcgcg tatcatttct ttattctaat aactcgcctg caaaaacgta
     9181 gaccgcatat cgccttgatg cggctcggaa aaaaataata agccctacaa aatattgagc
     9241 gagaaattat ggaaattgca tcgaataaag gcgtcattgc agacgcttcg accccggcgg
     9301 gtcgtgctgg aatgagtgag agcgagtggc gagaagcgat caaattcgac agtactgaca
     9361 ccggctgggt gattatgagt atcgggatgg cgattggcgc ggggattgtt tttctcccgg
     9421 tgcaggtcgg tttgatggga ttgtgggtat ttttgctctc atcggtgatt ggttacccgg
     9481 caatgtatct gtttcagcgg ttgtttatta atacgctggc agaatcaccg gagtgtaaag
     9541 attacccgag cgtcatcagc ggttatttag gtaaaaactg gggcatcctg ttaggtgcgc
     9601 tctatttcgt aatgctggtg atttggatgt tcgtctattc caccgccatc accaacgata
     9661 gtgcttccta cctgcatacc ttcggcgtga cggaaggggt gctgtcagac agtccctttt
     9721 atggtctggt actgatttgc attctggtgg cgatctcctc acgcggcgag aaattgttat
     9781 ttaaaatttc gaccggcatg gtgctgacca agctgctggt ggtcgcggcg ctgggcgtgt
     9841 cgatggtggg aatgtggcat ctgtataacg tcggttcgct accgccgctg gggctgctgg
     9901 tgaaaaacgc cattattacg ctgccgttta ccctgacgtc gattctgttt atccagacgt
     9961 taagcccgat ggtgatctct tatcgctcgc gggaaaaatc aattgaagtg gcgcggcata
    10021 aagcattgcg ggcaatgaat atcgcgtttg gcattttgtt tgtcaccgtc tttttctacg
    10081 ccgtgtcgtt cacgctggcg atgggacatg acgaagcggt aaaagcctac gagcagaata
    10141 tttccgcgtt ggcgattgcc gcacagttta ttagcggtga tggcgcagcg tgggtgaaag
    10201 tggtcagcgt cattctcaat atctttgctg taatgactgc attctttggc gtctatttag
    10261 gctttcgcga agcaacgcaa gggatcgtaa tgaacatcct gcgtcgcaag atgcctgccg
    10321 agaagattaa cgaaaatctc gttcagcgcg gcatcatgat tttcgccatt ttgctggcct
    10381 ggagcgccat cgtactgaac gcaccggtgt tgagcttcac ctctatctgt agcccgattt
    10441 tcggcatggt agggtgcctg atcccggcgt ggctggttta caaagtaccg gcattgcaca
    10501 aatacaaagg gatgtctctg tacctgatta tcgtcactgg tttgttgctt tgtgtttctc
    10561 cgttcctggc attttcttga tttacctgaa attttaaggt ttttaatatg tttgattcga
    10621 ctttaaatcc gttatggcag cgttacatcc tcgccgttca ggaggaagta aaaccggcgc
    10681 tgggatgtac tgaaccgatt tcactggcgc tggcggcggc ggttgctgcg gcagaactgg
    10741 aaggtccggt tgaacgtgta gaagcctggg tttcgccaaa tctgatgaag aacggtctgg
    10801 gcgtcaccgt tcccggcacg ggaatggtgg ggctgccaat tgcggcggcg ctgggagcgt
    10861 taggtggaaa tgctaacgcc gggctggaag tgctgaaaga cgcaactgcg caggcaattg
    10921 ccgatgccaa agcactgctg gcggcgggga aagtctcagt taagatccag gaaccttgcg
    10981 atgaaatcct cttctcacgc gccaaagtct ggaacggtga gaagtgggcg tgtgtcacca
    11041 tcgtcggcgg gcataccaac attgtgcata ttgagacgca caatggtgtg gtgtttaccc
    11101 agcaggcgtg tgtgacagag ggcgagcaag agtcgccgct gacggtgctt tcaagaacga
    11161 cgctggctga gatcctgaag ttcgtcaatg aagtcccgtt tgcggcgatc cgctttattc
    11221 tcgattccgc gaagctaaat tgtgcgttat cgcaggaagg tttgagcggt aagtgggggc
    11281 tgcatattgg cgcgacgctg gaaaaacagt gtgcacgcgg tttgctggcg aaagatctct
    11341 cttcatccat tgtgattcgt accagcgcgg catccgatgc gcgtatgggc ggcgctacgc
    11401 ttccggcaat gagtaactcc ggctcgggta accaggggat cactgcaaca atgcccgtgg
    11461 tggtggtagc agaacacttc ggggccgatg atgaacggct ggcgcgtgcg ctgatgcttt
    11521 cgcatttgag cgcaatttac atccataacc agttaccgcg tttgtctgcg ctgtgtgccg
    11581 caacgaccgc agcaatgggg gccgccgccg ggatggcatg gctggtggat gggcgttatg
    11641 aaatcatttc gatggcgatc agcagtatga tcggcgatgt cagcggcatg atttgcgatg
    11701 gagcgtcgaa cagctgcgcg atgaaggttt cgaccagtgc ttcggctgcg tggaaagcgg
    11761 tgttaatggc gctggatgat accgccgtga ccggcaatga ggggatcgtg gcgcatgatg
    11821 ttgagcagtc gattgccaac ctgtgtgcgt tagcaagcca ttcgatgcag caaacggatc
    11881 ggcagattat cgagattatg gcgagcaagg ccagataaac gccagttttg ggtatcgtgc
    11941 cggatgcgat gctggcgcgt cttatctggc ctacgaattg cacaatttgt tgatgttgtt
    12001 gggttttgta ggccggataa ggcgtttacg ccgcatccgg cacctgtctt tacgcggtag
    12061 cttcatcctc ggctaaacgc gtttcagcat ccgcgacgat ggcttccagt tcgctcaaca
    12121 tctcttcata gccaaaattt ttggcggtac gagcaggttc tttcgccacc acgggcttca
    12181 ctggctggcg ctttttggcc gattttttac tcatcatcat ctccctgtaa aaacaactag
    12241 acaggcaaat ctatcagcaa agcgcgcagt ggggaatcgg caaccagcgt aatgttagcc
    12301 tcgtcacgaa taaacgcccc atcaccgcag gttagcgccg ctttctcttc atgatgcgta
    12361 agcgcatgaa atttcccgtg aatcgattgc aaatacgcgc gtggtccatg caactggaaa
    12421 ttcgcacttt cgcctttgtc gagcacgata tggtgcagcc acacttgctg gcgcagttgc
    12481 aggctaccca ctgtgccctc tggcgaggcg attaattgct gcttgcccat attaagcgcc
    12541 agtttttgaa tcagcggatt ctctcgctgc gggcaggcat ccagccaaag ctgcattcgc
    12601 gttaacggtt tgtctttgct gagattgtgt tcgctatagc tgacacccgg ctgggtagag
    12661 agcagcaacg cctcaccagc gctggcctga acatgattgc cttcgctgtc gcgatactct
    12721 gcttctccat ccagaatcac atttaaaata tcgactttgg gataggttcg cggctgaaag
    12781 gcggcacctg gggccagcac ttcctggtta agcacgcgca gggaggcata gcctaacaat
    12841 ttcgggtcga agtagtgtcc aaaggaaaaa gtataccggg cctgcaacca tccgtagtct
    12901 gcttgtccac actgcctggc agttcgggta gtaatcataa ttcttgacct ctctttactt
    12961 ccttttatgg taaggggctg gacgctacat tgttagccag atattctgcc cggtatgttc
    13021 aaatttcctg aatgagaacg aaatggccaa agaaagggca ttaacgctgg aagcactacg
    13081 ggttatggat gcgatcgatc gccggggcag ttttgcggcg gcggcggatg agctgggacg
    13141 cgtgccttcc gcacttagct acaccatgca aaaactggaa gaagagctgg atgtggtgct
    13201 gtttgaccgc tcgggccatc gcaccaaatt caccaatgtc gggaggatgt tgctggagcg
    13261 aggacgcgtt ttgctggaag ccgcagataa actgactacc gatgcggaag ctctcgcgcg
    13321 cggttgggaa acgcatctca ccattgtgac cgaagcgctg gtgccgacac ctgccttttt
    13381 cccgttaatt gacaaactgg cggcaaaagc gaatacccaa ctggcaatca tcaccgaagt
    13441 gctggcgggg gcatgggaac ggctggagca ggggcgggcg gatattgtta tcgcgccgga
    13501 tatgcatttt cgctcctcgt cggagatcaa ctcgcgcaag ctctatacgt taatgaacgt
    13561 ctacgttgcc gcgcctgatc acccgattca tcaggagccg gaaccgttat ctgaagtgac
    13621 gcgcgtgaaa tatcgtggaa ttgcggtggc ggataccgct cgtgagcgcc cggtattgac
    13681 cgtacagcta ctggacaaac agccgcgttt aacggtgagc acgattgaag ataaacgtca
    13741 ggcgttactg gcggggcttg gcgtggcgac gatgccgtat ccgatggtcg aaaaagacat
    13801 cgcggaaggg cggttgcgtg tcgtcagccc ggaatcgacc agcgagatcg atattattat
    13861 ggcctggcgt cgtgacagta tgggggaggc gaaatcctgg tgtctgcggg aaattcccaa
    13921 actttttagc ggaaaataat atttgttatt gttaataaaa aggggcgaaa tatcgcccca
    13981 attctataat taatttgaac caaacttcgg atcgtttccg taacgattgc tgccagagtt
    14041 gccttctaaa caggcaaata caaacagaac taaccagcca atgatcggaa ccagatagag
    14101 aagcgcccac gcacctgaac ggtcagtatc gtgcagacga cgaacgcaca gagcaatgac
    14161 cggaatgatt gttgctgcca ggtaaatgag agaaaggaat ggaaactcta aacctaaaat
    14221 caattgaatg acattgataa tagcgccgac gatggcgtta atcagagtaa acatccagta
    14281 ctctttacga cgtgcgcgac cagagaaacc aacataattt tttaatacgg ctaaatacca
    14341 ctgcataaaa acatcccttt aattaaaagt gacgaaagtc tattccgggt attgtgtaaa
    14401 tgctattcgt atctttcttt ttcggatgtt ataaaggttt tgatatttaa gataaatata
    14461 tggttatttt tttataggta atttatataa cgacattgaa atattttaaa taaacattag
    14521 taaattattc attcatcaac ataatgataa aagccggatg agtcagacat ccggcgagta
    14581 gatttgtttt aaggttccag ttttggatca ggtccaaagc ggttttcgcc tggcgtaccc
    14641 gcctggcagt taaagacgat gataataagc cagccgataa acgggattaa gaacagcagt
    14701 gcccaccacg ccgagcggtc ggtgtcgtgc aggcggcgga actgaactgc ccaccacggt
    14761 aaaaacacca gaataccgta gattgtcgtc aggatgcctt cgccaccggc acgttgccag
    14821 cctaacattt tatccagcag ccccagcacg aacgtaaaga tgatgttgac cagaataaac
    14881 atccagtact ctttgcgccg cgcacgaccc cggaaaccga cataattttt tagtactttc
    14941 agataccagt ccatttttgc ctcaattttg ctcaatcact tgtttttaaa gcagcaaatt
    15001 aagaatagac gtgaatgaaa tggcagtaaa tattcgttca aggaattaat aaagtgccgt
    15061 aaagcgcgct aagactgatg acaagcgcaa aatcgccaga tgcgctacgc ttatctggcc
    15121 tacgcggcat tgtaacatat tgaatataaa cgttttgtag gttggagaag acgttcacgc
    15181 cgcatccgac ataaataaca cgcacttagc taataatcag aggcacctcg taaggtgcct
    15241 ttttttaacc gaagcgaaca tctcgtccat gcggttcatc gagatcctgc cacggaccaa
    15301 ttgaaataat ccccgtaggg ttgatggtct tatggctgcg gaagtaatga ttacggatgt
    15361 gattgaaatt gacagtttcg gcgatccccg gcatctggta gatatcgcgc agaaaaccgt
    15421 acagattcag gtaatcgctg atgcggtgct tatcacactt gaagtgggtc acatacaccg
    15481 gatcaaaacg gaccagcgtg gtccacaggc gaatatcggc ttcggttagc tgattgccgg
    15541 tcaggtaacg atgctggcct aaaatctgct ccagtcgcgc cagcgattca aacactttcg
    15601 ccaccgcctc gtcgtaagct tgctggctgg tggcaaaacc agctttatac acgccgttgt
    15661 taacggtgtc ataaatccag ccgttaagtt cgtcaatttt cgtttgcagg gctggtgggt
    15721 agtaatcacc cgctttcgcg cccagcgcat caaacgcggt attgaacatg cggatgattt
    15781 ccgctgattc gttgctgacg atggtgtggt tctttttgtc ccacagtacg ggaacagtaa
    15841 ctcgtccgct gtagtgtgga tcggcgtgga gataaagctg atacaaaaat tcatgttgat
    15901 agagtgtatc gccggttgca cccggaaaac tgtcatcaaa ggtccagccg ttttccagca
    15961 tcagcgggtt cactacagaa acggaaataa acggttccag tcctttgagt ttgcgcatga
    16021 tcagcgtgcg gtgcgcccac gggcaggcga gtgaaacata gagatgataa cgatctttct
    16081 ctgcgataaa accgcctgtg ccagtggggc ctggcgcgcc atcggcagtg agccagttac
    16141 gaaatgcgga agctgaacgt tgaaatttac cgccggtaga tttggtatcg taccaggtgt
    16201 catgccagac gccgtcaatc agttgaccca tatttctctc ctccaatagc aaaaacgagg
    16261 agcttcctcc tcgtttttta tcgttaagta tagcgcgtgc ttaccacttt ttattcagca
    16321 ggcggtcgat gctatacgcg cccggaccgg taattgccag cagcaggaat ccgccagaaa
    16381 ttgtcaggtt tttcatgaac atcagcgagt tgacgccttc agcaaagttg ctgtgaaata
    16441 aaaatgccgt cagcagcgta aagcccgcag taaacagggc tgtggtgcga gtcaggaaac
    16501 cgaacaggat tgccagacca ccaccaaact caagcagaat caccagcggc agcataaaac
    16561 ccgggacgcc catcgcttcc atatattgtt gggtacccgc gtaaccagta atttttcccc
    16621 agcctgcggt aataaacaga atcggcatta aaatgcgcgc taccagtaca ccaacatctt
    16681 ctaatttttt catcatactc tccactgcgc cgctcaggct gctgattgtt tttttgtgca
    16741 agggcgcgct attagcgtcg ttgctgtcga tggagagaat cataaacgtg gcgaagcgtg
    16801 attgttagca aggaaaactg tcaaatattt tcaaaaaatt tgagggataa ggccggaatt
    16861 tctccggcca gagaggaagt taaccgcgaa gctgttgttg cttgagggtt gttttcacca
    16921 gacgccaggc gctccataca ccaaaaccgc gtctggccca gcggaccagc atattaggat
    16981 ggcgaatcgt ccagatagcc atcacgctac tgccaaccag cgcccaggag cgcagactca
    17041 acagcatatt ccagcgacga tcgtaagcgc ctgttgcctc cagccattca cgacgactgg
    17101 cggaaagatc cagccgttgt tgctggatct ggctaagcag ttgcgcctta cgtcgttcac
    17161 gttcgacttt actgctcacg ggactcctcc tcgagcagct gccgatcgtt tgccagctca
    17221 tggcgcgtgt ggcgcagcaa cgtagactta cgcgatttac gtaacgtcca gatgccgcca
    17281 atcagtgcca gtagcagcaa caccacggtg gtggcaatca tcgcattcag gcgatattgc
    17341 ggatcaaccg cccagataat tagcaccatc aggctcataa gaccaaatgc agcgaaaagc
    17401 atcgtcaggc ccagcatcag taaaagttga aagagattcg ctttttcctc ttccagctcc
    17461 accaccgcca gccgcagacg tgtctccacc atttcaacca tgatggaaac aattcgctgc
    17521 ccgatgccca gaacgctttt accgggccct tgcgcgtgat gagtgtccgc cataattaac
    17581 gacgcgacag cagaacaccg agcactacac cgattgctgc gccaatgccc acgcccgtcc
    17641 acggattttc acgcacatac tcatcggcac gcgccgccgc gacacgggtt tgtttggcaa
    17701 tggcatcacc ggtttcaccc aggcgataac ggctctgttt cagtgcctgc tccgctttgc
    17761 tacgaatctt actcaactct tctttcgact tctcgccaga tgagctaagc acctcttcca
    17821 gcgtatcgga aagggatttc aactcagcac gcagatgttc cgtagtgtgt tctttcgaca
    17881 tagttttctc caggtgagta agtagtgact attgtgagtt agtagtcgcg cgcttccagc
    17941 tttttcagct cttcctgcgc ttctgccagt ttccgttcgc gtttggcaat cttatctgca
    18001 tcgccttttt gcttcgcctc ggctaaatct tgctggcgtt ccgccacctc atctttctgc
    18061 tttgcgattt tcttctgatg atcggcacgc agctggctat ctgaacagtt ggcccggact
    18121 tcactcaggg ctttattcag accgtcaata cgattctggt tttggtgttt ttcggcatag
    18181 ctgatctcct taaggatatt ttgctccttt tcctgacaca gggtagtggc ataactaccg
    18241 gcactaagag caaagagaga aacagctaaa gcgatgcggt atttcattct tgaaccttcc
    18301 attgtgattc ggacctatta caaaggaacg attgtccaga caacgaagcg gtcgtgttaa
    18361 ccaccgcctc attaatcata gacagttacg gtgaaatcac caaagtgagt gatctgtttc
    18421 ggataacagg aagttatccg aagcgatgag agttatcccg caaccgggtc agccactgca
    18481 tagcctgact gttattgtcc tgctgcgcaa tgatgtagcc atggggcaat gttctgtcga
    18541 ggaccgcttt tgcggcggcg ctctggtcgc tggagtcaaa ggtaattaac agagtgtcgt
    18601 ttttgggggt gatacttttg aaaggaatgc cgtgagcgtc aaggtgatgc cagattgaaa
    18661 aaccgtctgg catcgttgtc ccttgatgaa cggcacgaat cgccagcgta gactcttgct
    18721 ggcgaaccgc ggaccaggcc agcaacagtg tgccgaccag aagtaaaaca gcgccggacc
    18781 aggctagctg gcgaagcgac atgcgaggta tttgcatccc ttacccccga tttccatatt
    18841 tctttttcca taacacgacc agagaacctg ccaggccaaa caccagcagc accaccggga
    18901 gcagcatcag gcatgacatc agctggtcct cgtactttaa aaataccggc gttttgccga
    18961 gcatgtaacc cagagttgtc aggatcaata cccacaacaa accactcatc cagttgaaga
    19021 actgaaagcg cgcgttattc agccctgata acccggcaat cgtcggcagc agtgttctga
    19081 caaacgcaat aaagcgacca attaacagcg ccgataaacc gtgtttatga aaaagatggt
    19141 gtgcgcgttg atgataatgc gcgggtaaat gagatagcca gttttgtacg gtgcgggtat
    19201 tgcccagcca tcgcccctga atatagctga cccagcagcc gaggctggcg gcaacggtca
    19261 gcagcagaat cgtttgcgga tagcccatcg cgcctttcgc aatcaataca ccgaccaaca
    19321 ccagtaagct gtcgcccggt aaaaaggctg ccggaagcaa gccgttttca aggaacaaaa
    19381 tgacaaacaa gacaaaatac aacatgccaa tcatcgacgg attggccagg gtttcaaaat
    19441 cctgcgccca cagggcttgc agcaattggg tcaaaagttc cattcattat tcctggtgaa
    19501 tcggttaacg ccacgctggt tttaaatgat gcactaccgc aatgttattg ctgtcttggt
    19561 atgttctgcg ggtttgatac atcaacacag cctgaactat tccctgttaa atcgtgagat
    19621 aagaaagcat tcaaaagcat aaattcaaat tgaatctaat tgtaacaaag gtcaacgttg
    19681 ttcgttatca aaattacatg tccgtggagt ctgattttgc tgaatgcagg ggagggaggc
    19741 gagaggattt acacaggctg acactttata cttttcgctt accgacgaga caaacaggcg
    19801 cactgtagcg cctgtcggac aggaatttca tttactgccg ctggtggctg tatcgagatg
    19861 caccaccggg ttttcggcga acagatagcg gtcggcattg aactcgaaat cgtcgctggt
    19921 ttcgttaaat aacatgatct tggtgttttc caggtgctgc cacattgcca gcttagcagc
    19981 atgaggatct ttgcgaatca gcgccttgag gatttgatcg tggtcatcac accagttatc
    20041 gacggtacgg gcatcaatgt gttcgtgcag ttttttccag tacgggttat gactacgctg
    20101 ggtccacatt ttttccacga tagccgccag ggcggagttc tgcgtcgcca gggctacctg
    20161 aatgtggaac tgcaaatccc actcggaatc acggaagcat tgttcgccgc gcgcctgctc
    20221 ctggatggcc atcagtttca tgatgtcctg tttcgtcacc tgagttgccg cgaactcggc
    20281 aatattactt tcgatgagct ggcgagcctg gagcaactca aacggaccgt aattggcgaa
    20341 ttccatatta ttgtcagtcg cctgctgatg gcgcggctgg ttggaaacca catgaatacc
    20401 tgaaccttta cgcacttcga catagccttc aacttccagc atgatgatgg cttcacgaac
    20461 cacggtgcgg ctgacgttct tttcatcggc aataaagcgt tcagcaggca gtttatcacc
    20521 caccagatag acgccctgtt cgatgcgctc tttcagatca gcggcaagtt gttgatacaa
    20581 acggcgtggt tcagtgattt ccatatgcgc tccaggcatg atacggcaga ctatatttgt
    20641 tataccactt ttgcgagttt agctctactc acagtgatga aaaagccgcc tgagaaggcg
    20701 gctttgaagg gaaactaaaa cttacacgaa gccttaatgt tgcggtgcgg gatcgttatg
    20761 tgattcctgc gccacctcaa tcgccggttt gttctgcaac acggtccaga taaccagcgc
    20821 acctaacagg tcgaacactg ccagaactgc gaacagcggg ctgaagccga tggtgtcagc
    20881 cagtgcaccg actaccagtg caaacagcgt acttgccagc catgcggaca tcccggttaa
    20941 accgttcgcc gtcgccactt cgttacgacc gaacacgtca gacgagagcg taatcagtgc
    21001 gccagacagt gcctggtgag caaaaccacc gatacacagc agcataatcg cgacatacgg
    21061 gttggtgaac agaccgatca tacccgggcc aatcatcagc accgcaccca gcgttacgac
    21121 catcttacgg gaaacgatca ggttcacacc aaaccaacgc tggaacagtg gtggcaggta
    21181 accaccgagg atacaaccga ggtcagcaaa cagcatcggc atccaggcaa acatcgcgat
    21241 ctctttcagg ttaaagccgt aaactttaaa catgaacagc gggatccacg cgttaaaagt
    21301 accccatgcc ggttctgcca ggaagcgcgg cagcgcgata ccccagaact gacggttacg
    21361 caggatctga ccaacggaca ttttcttcgc tgtgtcaacc tggtgctggg cttcctgacc
    21421 attaataatg tagtcgcgtt cttcatcggt cagatgtttc tggtcgcgcg gatgtttata
    21481 gaaaatcagc catgccatcg cccagataaa gctcaacgca ccggagatga taaatgccat
    21541 ctgccagctg tgcatcacga ttgcccatac caccagcggc ggcgcaatca tcgcaccaat
    21601 cgaagaacct acgttaaagt aacctaccgc gatggaacgc tctttcgccg ggaaccattc
    21661 ggagctggct ttcagacccg ccggaatcat cgcggcttcc gcggcaccga ccgcaccacg
    21721 agcaacagcc aggccgcccc agctacctgc cagcgcggtt gcaccacaga acacggccca
    21781 cagcacagca aacattgcat aaccgatttt cgtacccagc acatccagta catagcccgc
    21841 taccggttgc atgaccgtat aagcagcaga ataggctgcg atgatatagg aatactgttg
    21901 ggtggagatg tttaactctt ccatcagagt tggcgcagct gccgccacag tgttacgcgt
    21961 caggtaacca agcacggtgc cgagcgtcac cagtgcgatc atataccaac gtaacccttt
    22021 aattttacgc atgtaaaacc tcatcgtttt gttatatccg caggaagcgc ggccatcatc
    22081 ccgccagagg ctgaagatgt tagttacgac cgggtaaccg aaaaaaaacc ggcacgcccc
    22141 gaacgttgcc atgaaagttt cgtgctaatt cggcttccgt accggttaac ccgattcatc
    22201 taacaaatga atgaatcggc aatttgcatt ccgtcgactt atgatttgcg acggcagaaa
    22261 gataacttgt catacaactt taaaaggtga gagccatcac aaatgtgcga atatttgtag
    22321 ggacattacc tgacgacggc gaggccagta ctggcgcggc ctgcagcgag atttaccact
    22381 ttgagagtaa tttttttaac tacgtttatt gatctaactc acgaaaatat cttcggactc
    22441 tggaaattgg tgtgataact ttgtcagcat cgcaccataa gcaagctagc tcactcgttg
    22501 agaggaagac gaaaatgact ccgtttatga ctgaagattt cctgttagat accgaatttg
    22561 cccgccgtct gtatcacgac tacgcaaaag accagccgat tttcgattac cattgccatt
    22621 tgccgccgca gcagattgcg gaagactatc gttttaaaaa cctgtatgac atctggttga
    22681 agggcgatca ctacaaatgg cgcgctatgc gtaccaacgg tgtggccgag cgtctgtgca
    22741 ccggtgatgc gtctgaccgt gaaaaatttg acgcctgggc ggcgactgtt ccgcacacta
    22801 ttggcaaccc gttataccac tggacgcacc tcgaactgcg tcgtccgttt ggtatcactg
    22861 gcaaattgct ttctccgtca actgccgatg aaatctggaa cgaatgtaac gaactgctgg
    22921 cgcaggataa cttctctgcg cgcggcatca tgcagcagat gaacgtgaaa atggtcggca
    22981 ccaccgatga cccgatcgat tctctggagc atcacgcaga gatcgccaaa gacggctctt
    23041 tcaccatcaa agtgctgccg agctggcgtc cggacaaagc ctttaacatc gaacaggcga
    23101 cctttaacga ctacatggcg aagctgggtg aagtctctga taccgacatc cgccgctttg
    23161 ctgatctgca aactgccctg accaaacgtc tggatcactt cgccgctcac ggctgtaaag
    23221 tgtctgacca cgcgctggat gtggtgatgt ttgctgaagc gaacgaagcg gaactggaca
    23281 gcattctggc gcgccgtctg gcaggcgaaa ccctgagcga gcacgaagtg gcacagttca
    23341 aaactgcggt gctggtgttc cttggtgccg aatacgcacg tcgcggctgg gtacagcagt
    23401 accacatcgg cgcgctgcgt aataacaacc tgcgtcagtt caaactgctg gggccggatg
    23461 taggctttga ctccatcaat gaccgtccga tggcagaaga gctgtctaag ctgctgagca
    23521 agcagaacga agaaaacctg ctgccgaaaa ccattctcta ctgcctgaac ccgcgcgata
    23581 acgaagtgct gggcaccatg atcggtaact tccagggcga aggtatgccg ggcaaaatgc
    23641 agttcggttc cggctggtgg tttaacgatc agaaagacgg tatggaacgt cagatgaccc
    23701 aactggcgca gctcggtctg ttgagccgct ttgtcggtat gctgactgac agccgtagct
    23761 tcctgtcata cacccgtcac gaatacttcc gccgcattct gtgccagatg atcggtcgct
    23821 gggtggaagc gggcgaagca ccggcggaca tcaacctgct gggcgagatg gtgaaaaata
    23881 tttgctttaa caatgcgcgt gactacttcg ccattgaact gaactaaggt ctggggttga
    23941 tatgcaatac atcaagatcc atgcgctgga taacgtcgcg gtcgctttag cagatttggc
    24001 tgaaggcaca gaagtcagtg tcgataacca gactgttacg ctgcgccagg atgttgctcg
    24061 tggacataaa tttgcgttaa cggatatcgc aaaaggggcc aacgtcatca aatatggcct
    24121 gccgattggt tatgcattgg cggatattgc ggcgggggaa cacgtccacg cccacaatac
    24181 gcgcacgaat ctgagcgatc tggatcagta tcgctatcaa cctgattttc aggatctgcc
    24241 tgcgcaagcg gcagatcgtg aagtgcagat ctatcgtcgc gctaacggcg atgtcggggt
    24301 gcgtaatgag ctgtggatcc tgccaaccgt gggctgtgtc aacggcatcg cgcggcagat
    24361 ccaaaatcgt ttcctgaaag agaccaacaa cgccgaaggt actgacggcg tgttcctctt
    24421 cagccacacc tacggctgct cacagctggg cgacgatcac atcaataccc gcaccatgct
    24481 gcaaaacatg gtgcgccacc cgaacgcagg cgcagtgctg gtgattggtc tgggctgtga
    24541 aaacaaccag gttgccgcat tccgtgaaac gctgggcgat atcgatcctg aacgcgttca
    24601 tttcatgatc tgccaacagc aggatgatga gatcgaagcc gggatcgcgc atttgcatca
    24661 gctgtataac gtgatgcgca acgataaacg cgagccaggc aaactcagcg aactgaagtt
    24721 tggtctggag tgcggtggtt ctgacggtct ttctggtatt actgctaacc cgatgctggg
    24781 gcgtttctct gactacgtga ttgctaacgg tggtactacc gtactgaccg aagtgccgga
    24841 gatgtttggc gcagagcagt tgctgatgga ccattgccgc gacgaagcaa cgtttgaaaa
    24901 actggtcacc atggtcaacg acttcaagca gtactttatt gcccatgacc agccgatcta
    24961 cgaaaaccca tcaccgggga acaaagcagg cggtatcacc acgctggaag ataaatcgct
    25021 tggctgtacc cagaaagcgg gttccagtgt cgtggttgac gtgctgcgtt acggcgagcg
    25081 tctgaaaacg ccggggctga acttgttaag tgcgccgggt aacgatgccg tagcgaccag
    25141 cgccctggcg ggtgcgggct gccatatggt gctgttcagt actggtcgtg gcacgccgta
    25201 tggtggattt gtgccgacgg tgaaaatcgc caccaacagt gaactggcgg cgaagaaaaa
    25261 acactggatc gactttgacg cgggtcagct gatccacggt aaagcgatgc cgcagttgct
    25321 ggaagaattc atcgatacca tcgttgagtt tgccaacggt aagcaaacct gtaacgagcg
    25381 taacgacttc cgcgaactgg cgatcttcaa aagcggcgta acgctataaa gtcgaaatga
    25441 gttgttaata aaaaacggcg tttcataatg tgaagcgccg tttgcttttc cagacctgct
    25501 aaggatttat catgaccgcg tattggctgg cccagggcgt gggtgtcatc gcctttctga
    25561 ttggtatcac aacatttttc aaccgtgacg aacgccgctt taaaaagcag ctttcggtct
    25621 atagcgccgt tattggcgta catttttttc ttctgggcac ctatcccgcc ggtgccagtg
    25681 ccatccttaa tgccattcgt acgctgatta ctttacgcac gcgcagctta tgggtaatgg
    25741 cgatttttat tgtgctgact ggcggaattg gcctcgcgaa gttccatcat cctgtcgaac
    25801 tactgccggt aatcggcacg attgtcagta cctgggcgct gttccgctgt aaagggctga
    25861 ccatgcgctg cgtaatgtgg ttttcaacgt gttgctgggt gattcacaac ttctgggcgg
    25921 ggtcgatagg cggcacgatg attgagggga gttttctgct gatgaatggt ctgaatatca
    25981 ttcgtttctg gcggatgcag aaaaggggaa ttgatccgtt taaagtagag aaaacccctc
    26041 ccgccataga cgaaaggggt taaacaatta attacgcagg gcgctatttg ccagacgatc
    26101 gtcttccgcc tggcaagctg ccgcagtgaa cagcacgtca gttgaagagt tcagcgcggt
    26161 ttcgcaagag tcctgcaata cgccgatgat aaagccgacg gcaaccacct gcatggcgat
    26221 atcgttcgaa ataccgaaca tattacaggc cagtgggatc agcagcagag acccccccgc
    26281 cacgccggat gcgccacagg cacacagaga agccactacg ctcaacagca gcgctgtggg
    26341 cagatcgacc ggaataccca gcgtattaac cgcagccagc gtcaacacgg taatggtgat
    26401 tgctgcgccc gccatattga tggtggctcc cagcggaata gaaacagaat aggtatcgcg
    26461 atccagattc agcttttcac acagcgccat attcaccgga atgttcgctg cagagctgcg
    26521 ggtgaagaag gcatacacac cgctttcacg caggcacagc agcaccagcg ggaacgggtt
    26581 acgacgaatt ttccaccaca ccagcaatgg gttaaccacc agcgccacca gcagcataca
    26641 gccaaccagc acgaccagca gttgcgcgta gccccacagc gtggagaaac cggtggttgc
    26701 cagggtagaa gaaaccagac caaaaatacc gatcggtgcg aagcgaatca ccagtttcac
    26761 cataaaggtg acggcattcg acatatcgtt gatcaggttt ttggtggtct cgttaccgtg
    26821 acgcagtgcg aaaccgaggc caattgccca caccaggatc ccgatatagt taccttttag
    26881 cagcgcgtca atggggttgg aaaccatgct cattaccagc ccgcgcatta cttctacaat
    26941 gcctgacggc ggcgaaatat cacccgcgct actggacaag tgcagggtag aagggaaggc
    27001 aaaactgaag attactgcgg ccagagcagc agagaaggtg cccagcagat agaggaacaa
    27061 aatagggcgg atattggttt tctgcccgtg ctggtggtta gcaatagatg ccattaccag
    27121 catcaacacc aggatggggg caacggcttt cagtgcgccg acgaacaaag tacctaacag
    27181 accaacagct tccgccgcgg gttttgagat ccatgccaga agaatcccca gaacaaggcc
    27241 gaccaggatt tgttttacca ggctgccatg agccagacgc cggaatagcc ccggtgaacg
    27301 ttgcgtagtc atttttcgat cctttcattg tgttgtcgca catccttggt gcgttctgtc
    27361 gacgcatctt tcaggatgta acaaagtgtt tgcaaggtcg agtataagga aagttacagc
    27421 ttcgggaagc gaaaaatgct ggattttaaa gagaggtcat attttgcaac ttaatggtta
    27481 catttatttg acataaacag agggaaggac tggctactaa ggctgattag ataacatatg
    27541 ggcaaaacta tttggaatat gagaacggtt gcgctccttt aatgtgc
//
LOCUS       DAZGQL010000074        26480 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.074, whole genome shotgun sequence.
ACCESSION   DAZGQL010000074 DAZGQL010000000
VERSION     DAZGQL010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26480
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.074"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            62..1531
                     /gene="gspA"
                     /locus_tag="ACHKIR_003434"
     CDS             62..1531
                     /gene="gspA"
                     /locus_tag="ACHKIR_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspA"
                     /protein_id="HFO9991120.1"
                     /translation="MSTRREVILSWLREKRQTWRLCYLLGEAGSGKTWLAQQLQKDKH
                     RRVITLSLVVSSQGKAAWIVTDDNAAEQGCRDSAWTRDEMAGQLLHALHRTDSRCPLI
                     IIENAHLNHRRILDDLQRAISLIPDGQFLLIGRPDRKVERDFKKQGIELVSTGRLMEH
                     ELKASILEGQNIDQPDLLLTARVLKRIALLCRGDRRKLALAGETISLLQQAEQTSVFT
                     AKHWRMIYRVLGDNRPRKMQLAVVMSGTIIALTCGWLLLSSFTDPFPVPAWLVPVTPV
                     VKQDMTKDIAHVVMRDSEALSVLYGVWGYEVPADSAWCDQAVRAGLACKSGNASLQTL
                     VDQNLPWIASLKVGDKKLPVVVVRVGDATVDVLVGQQTWTLTHQWFESVWTGDYLLLW
                     KMSPEGESTITRDSSEEEILWLETMLNRALHISTEPSAEWRPLLVEKIKQFQKSHHLK
                     TDGVVGFSTLVHLWQVAGESAYLYRDEANISPETTVKGK"
     gene            1533..1952
                     /gene="gspB"
                     /locus_tag="ACHKIR_003435"
     CDS             1533..1952
                     /gene="gspB"
                     /locus_tag="ACHKIR_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative general secretion pathway protein GspB"
                     /protein_id="HFO9991121.1"
                     /translation="MFEFYIAAREQKETGHPGIFSRQKHSTIIYVICLLLICLWFAGM
                     VLVGGYARQLWALWIVKAEVTVEAETPAFKQSTQHYFFKKQPLPVVESVEEEDDPDVA
                     VENAPSSSEDEENTVEESDEKAGLRERVKNALNELER"
     gene            2190..2501
                     /gene="rpsJ"
                     /locus_tag="ACHKIR_003436"
     CDS             2190..2501
                     /gene="rpsJ"
                     /locus_tag="ACHKIR_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFO9991122.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2534..3163
                     /gene="rplC"
                     /locus_tag="ACHKIR_003437"
     CDS             2534..3163
                     /gene="rplC"
                     /locus_tag="ACHKIR_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFO9991123.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            3174..3779
                     /gene="rplD"
                     /locus_tag="ACHKIR_003438"
     CDS             3174..3779
                     /gene="rplD"
                     /locus_tag="ACHKIR_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFO9991124.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3776..4078
                     /gene="rplW"
                     /locus_tag="ACHKIR_003439"
     CDS             3776..4078
                     /gene="rplW"
                     /locus_tag="ACHKIR_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFO9991125.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            4096..4917
                     /gene="rplB"
                     /locus_tag="ACHKIR_003440"
     CDS             4096..4917
                     /gene="rplB"
                     /locus_tag="ACHKIR_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFO9991126.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4934..5212
                     /gene="rpsS"
                     /locus_tag="ACHKIR_003441"
     CDS             4934..5212
                     /gene="rpsS"
                     /locus_tag="ACHKIR_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFO9991127.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            5227..5559
                     /gene="rplV"
                     /locus_tag="ACHKIR_003442"
     CDS             5227..5559
                     /gene="rplV"
                     /locus_tag="ACHKIR_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFO9991128.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5577..6278
                     /gene="rpsC"
                     /locus_tag="ACHKIR_003443"
     CDS             5577..6278
                     /gene="rpsC"
                     /locus_tag="ACHKIR_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFO9991129.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            6291..6701
                     /gene="rplP"
                     /locus_tag="ACHKIR_003444"
     CDS             6291..6701
                     /gene="rplP"
                     /locus_tag="ACHKIR_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFO9991130.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6701..6892
                     /gene="rpmC"
                     /locus_tag="ACHKIR_003445"
     CDS             6701..6892
                     /gene="rpmC"
                     /locus_tag="ACHKIR_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFO9991131.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6892..7146
                     /gene="rpsQ"
                     /locus_tag="ACHKIR_003446"
     CDS             6892..7146
                     /gene="rpsQ"
                     /locus_tag="ACHKIR_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFO9991132.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            7311..7682
                     /gene="rplN"
                     /locus_tag="ACHKIR_003447"
     CDS             7311..7682
                     /gene="rplN"
                     /locus_tag="ACHKIR_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFO9991133.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7693..8007
                     /gene="rplX"
                     /locus_tag="ACHKIR_003448"
     CDS             7693..8007
                     /gene="rplX"
                     /locus_tag="ACHKIR_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFO9991134.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            8022..8561
                     /gene="rplE"
                     /locus_tag="ACHKIR_003449"
     CDS             8022..8561
                     /gene="rplE"
                     /locus_tag="ACHKIR_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFO9991135.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8576..8881
                     /gene="rpsN"
                     /locus_tag="ACHKIR_003450"
     CDS             8576..8881
                     /gene="rpsN"
                     /locus_tag="ACHKIR_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFO9991136.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8915..9307
                     /gene="rpsH"
                     /locus_tag="ACHKIR_003451"
     CDS             8915..9307
                     /gene="rpsH"
                     /locus_tag="ACHKIR_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFO9991137.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            9320..9853
                     /gene="rplF"
                     /locus_tag="ACHKIR_003452"
     CDS             9320..9853
                     /gene="rplF"
                     /locus_tag="ACHKIR_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFO9991138.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9863..10216
                     /gene="rplR"
                     /locus_tag="ACHKIR_003453"
     CDS             9863..10216
                     /gene="rplR"
                     /locus_tag="ACHKIR_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFO9991139.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            10231..10734
                     /gene="rpsE"
                     /locus_tag="ACHKIR_003454"
     CDS             10231..10734
                     /gene="rpsE"
                     /locus_tag="ACHKIR_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFO9991140.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10738..10917
                     /gene="rpmD"
                     /locus_tag="ACHKIR_003455"
     CDS             10738..10917
                     /gene="rpmD"
                     /locus_tag="ACHKIR_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFO9991141.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10921..11355
                     /gene="rplO"
                     /locus_tag="ACHKIR_003456"
     CDS             10921..11355
                     /gene="rplO"
                     /locus_tag="ACHKIR_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFO9991142.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            11363..12694
                     /gene="secY"
                     /locus_tag="ACHKIR_003457"
     CDS             11363..12694
                     /gene="secY"
                     /locus_tag="ACHKIR_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFO9991143.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12726..12842
                     /gene="rpmJ"
                     /locus_tag="ACHKIR_003458"
     CDS             12726..12842
                     /gene="rpmJ"
                     /locus_tag="ACHKIR_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFO9991144.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12989..13345
                     /gene="rpsM"
                     /locus_tag="ACHKIR_003459"
     CDS             12989..13345
                     /gene="rpsM"
                     /locus_tag="ACHKIR_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFO9991145.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            13362..13751
                     /gene="rpsK"
                     /locus_tag="ACHKIR_003460"
     CDS             13362..13751
                     /gene="rpsK"
                     /locus_tag="ACHKIR_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFO9991146.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13785..14405
                     /gene="rpsD"
                     /locus_tag="ACHKIR_003461"
     CDS             13785..14405
                     /gene="rpsD"
                     /locus_tag="ACHKIR_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFO9991147.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14431..15420
                     /gene="rpoA"
                     /locus_tag="ACHKIR_003462"
     CDS             14431..15420
                     /gene="rpoA"
                     /locus_tag="ACHKIR_003462"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFO9991148.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15461..15844
                     /gene="rplQ"
                     /locus_tag="ACHKIR_003463"
     CDS             15461..15844
                     /gene="rplQ"
                     /locus_tag="ACHKIR_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFO9991149.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            15951..16319
                     /locus_tag="ACHKIR_003464"
     CDS             15951..16319
                     /locus_tag="ACHKIR_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HFO9991150.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILADDSHVPPELR
                     AGYRLLKNAGCLLPELEQRREAIQLLDILRGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLNKINDN"
     gene            16330..16755
                     /gene="zntR"
                     /locus_tag="ACHKIR_003465"
     CDS             16330..16755
                     /gene="zntR"
                     /locus_tag="ACHKIR_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HFO9991151.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            16811..17029
                     /gene="arfA"
                     /locus_tag="ACHKIR_003466"
     CDS             16811..17029
                     /gene="arfA"
                     /locus_tag="ACHKIR_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HFO9991152.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVERNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGAC"
     gene            complement(17026..17436)
                     /gene="mscL"
                     /locus_tag="ACHKIR_003467"
     CDS             complement(17026..17436)
                     /gene="mscL"
                     /locus_tag="ACHKIR_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFO9991153.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPTPTKEEVLLTEIRDLLKEQNNRS"
     gene            complement(17566..18942)
                     /gene="trkA"
                     /locus_tag="ACHKIR_003468"
     CDS             complement(17566..18942)
                     /gene="trkA"
                     /locus_tag="ACHKIR_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HFO9991154.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(18964..20253)
                     /gene="rsmB"
                     /locus_tag="ACHKIR_003469"
     CDS             complement(18964..20253)
                     /gene="rsmB"
                     /locus_tag="ACHKIR_003469"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFO9991155.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLNWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRQQEELLAEFNASDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPPADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(20299..21246)
                     /gene="fmt"
                     /locus_tag="ACHKIR_003470"
     CDS             complement(20299..21246)
                     /gene="fmt"
                     /locus_tag="ACHKIR_003470"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFO9991156.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(21261..21770)
                     /gene="def"
                     /locus_tag="ACHKIR_003471"
     CDS             complement(21261..21770)
                     /gene="def"
                     /locus_tag="ACHKIR_003471"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFO9991157.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            21900..23024
                     /gene="dprA"
                     /locus_tag="ACHKIR_003472"
     CDS             21900..23024
                     /gene="dprA"
                     /locus_tag="ACHKIR_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HFO9991158.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLSWLEQPNHHLIPADSEFYPPQLLATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLATSLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPLGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQEDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            22996..23469
                     /gene="smg"
                     /locus_tag="ACHKIR_003473"
     CDS             22996..23469
                     /gene="smg"
                     /locus_tag="ACHKIR_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein Smg"
                     /protein_id="HFO9991159.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFDREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDTAEFDLEDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            23498..24040
                     /locus_tag="ACHKIR_003474"
     CDS             23498..24040
                     /locus_tag="ACHKIR_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HFO9991160.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            24045..24617
                     /gene="tsaC"
                     /locus_tag="ACHKIR_003475"
     CDS             24045..24617
                     /gene="tsaC"
                     /locus_tag="ACHKIR_003475"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HFO9991161.1"
                     /translation="MNNNLQGDAIAAAIDVLNEKRVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            24622..25440
                     /gene="aroE"
                     /locus_tag="ACHKIR_003476"
     CDS             24622..25440
                     /gene="aroE"
                     /locus_tag="ACHKIR_003476"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFO9991162.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFSAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GIGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKLLQQELSA"
     gene            25437..25694
                     /locus_tag="ACHKIR_003477"
     CDS             25437..25694
                     /locus_tag="ACHKIR_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HFO9991163.1"
                     /translation="MNQAIQFPDREEWDENKRCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(25670..26224)
                     /locus_tag="ACHKIR_003478"
     CDS             complement(25670..26224)
                     /locus_tag="ACHKIR_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFO9991164.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSAKNYVKWKDEYLDQGNQTQP"
     gene            complement(26268..>26480)
                     /locus_tag="ACHKIR_003479"
     CDS             complement(26268..>26480)
                     /locus_tag="ACHKIR_003479"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9991165.1"
                     /translation="EFQDFFSLLLRPSCEVFHVVVSTEAHYRDPNFLHKYFFDLFFCL
                     LLFHPFCCIRTQNGAFLHAKRLAQGQ"
BASE COUNT         6745 a         6256 c         6916 g         6563 t
ORIGIN      
        1 taataaatgc atacattatt tttatgatta aggataatca ataattaaaa caggaagttc
       61 tatgtctacg agaagagaag ttattctttc ctggttgcgt gagaaacgtc agacctggcg
      121 tctgtgctat ttgttgggtg aggctggaag tggaaaaacc tggctggcgc agcaactaca
      181 aaaagataaa catcgccgtg tgattacttt aagcctcgtt gtttcctcgc aaggtaaggc
      241 cgcatggatc gttaccgacg ataatgcggc tgaacagggc tgccgtgaca gcgcctggac
      301 gcgagatgag atggcgggtc aattactgca tgcgcttcat cgaactgata gtcgctgccc
      361 tcttattatt attgaaaatg ctcacctgaa tcaccgccgc atacttgatg atctccaacg
      421 cgcgatatct cttattcctg acggtcaatt tttgctgatc ggcagacccg atcgaaaagt
      481 cgaacgtgat tttaaaaaac agggcattga acttgtctca acaggacgtc tgatggagca
      541 cgagcttaag gcaagcatcc ttgaaggtca gaatattgac cagcccgatc tccttttaac
      601 tgccagagtt ctgaaacgga tagctttatt atgtcgggga gatcgcagaa agctggcgct
      661 tgccggcgaa acaattagct tattacagca agccgagcag accagcgttt tcactgcaaa
      721 acattggcga atgatttacc gcgtattagg tgataaccgc ccccggaaaa tgcagcttgc
      781 cgtggtgatg agtggaacca ttatcgcact tacctgtggt tggctactgc tgtcctcttt
      841 cacagatccg ttccccgttc ctgcgtggct ggtacccgta acgccagtag ttaaacaaga
      901 tatgactaag gatattgctc atgtagtgat gcgcgatagt gaggcgctaa gcgtgttata
      961 cggcgtatgg gggtacgagg tcccagctga ttcggcctgg tgcgatcagg cagtaagagc
     1021 ggggctggca tgtaaaagtg ggaatgcttc tttacaaaca ttggtagatc agaatttgcc
     1081 ctggatcgca tcgcttaaag tcggggacaa aaaattgcct gttgtggtcg ttcgcgtcgg
     1141 tgatgctacc gttgatgtcc tcgtagggca gcaaacctgg acgctgacac atcaatggtt
     1201 tgagtcggta tggacgggtg attatcttct gttatggaaa atgtcgccgg aaggggagag
     1261 taccattacg cgtgatagca gcgaggaaga gattctctgg ctggaaacga tgttaaaccg
     1321 cgcattgcat atttcgacag aaccttcggc agagtggcgt cccttgttgg tagaaaaaat
     1381 aaagcaattt caaaaaagcc accacttgaa aactgacggt gttgttggct tttcaacgtt
     1441 agtacatctc tggcaggtgg caggggagag tgcctactta tatcgggatg aagcaaatat
     1501 ttccccggaa acaacagtga aggggaaatg acatgtttga attctatatc gccgcgcgtg
     1561 agcagaaaga aacaggtcac cctggtatct tctcccgaca aaaacacagc accataatat
     1621 atgtcatttg tttattgctg atttgcctgt ggtttgcagg aatggtgttg gttggtgggt
     1681 atgccaggca gctatgggcg ctttggatag taaaagcaga agtcactgta gaggctgaaa
     1741 cgcccgcttt taaacaatca acacaacact atttcttcaa aaaacagcca ctccccgtcg
     1801 tcgaatccgt cgaggaggaa gatgacccag acgtcgcggt tgagaatgct ccctcttctt
     1861 ctgaagacga agaaaatact gtagaagaaa gcgacgagaa agccggttta agagagcgtg
     1921 tcaaaaatgc gctgaacgaa cttgagagat aacccgaagg ctgtttactt tactagcaat
     1981 acgcttgcgt tcggtggtta agtatgtata atgcgcgggc ttgtcgtagt tgacagcagg
     2041 ttcaatctga acctcagtta gccgaatttg gctacctaac aatgctccca atcggggagc
     2101 tacgtaagaa cggttacact ctcccatcaa tcgtaatggg tctgaggagt aatcattttc
     2161 gtttataaaa taattggagc tctggtctca tgcagaacca aagaatccgt atccgcctga
     2221 aagcgtttga tcatcgtctg atcgatcaag caaccgcgga aatcgtcgag actgccaagc
     2281 gcactggtgc gcaggtccgt ggtccgatcc cgctgccgac acgcaaagag cgcttcactg
     2341 ttctgatctc cccgcacgtc aacaaagacg cgcgcgatca gtacgaaatc cgtactcact
     2401 tgcgtctggt tgacatcgtt gagccaaccg agaaaaccgt tgatgctctg atgcgtctgg
     2461 atctggctgc cggtgtagac gtgcagatca gcctgggtta atcaggtcat tgagcgattg
     2521 agaggttgaa acaatgattg gtttagtcgg taaaaaagtg ggtatgaccc gtatcttcac
     2581 agaagacggc gtttctatcc cagtaaccgt aatcgaagtt gaagcaaacc gcgttactca
     2641 ggttaaagac ctggctaacg atggctaccg tgctattcag gtaaccaccg gtgctaaaaa
     2701 agctaaccgt gtgaccaagc ctgaagctgg ccacttcgct aaagctggcg tagaagctgg
     2761 ccgtggtctg tgggaattcc gcctggctga aggcgaagag ttcactgtag gtcagagcat
     2821 tagcgttgaa ctgtttgctg acgttaaaaa agttgacgta actggcacct ctaaaggtaa
     2881 aggtttcgca ggtaccgtta agcgctggaa cttccgtacc caggacgcta ctcacggtaa
     2941 ctccttgtct caccgcgttc cgggttctat cggtcagaac cagactccgg gcaaagtgtt
     3001 caaaggcaag aaaatggcag gtcagatggg taacgaacgt gtaaccgttc agagccttga
     3061 cgtagtacgc gttgacgctg agcgcaacct gctgctggtt aaaggtgctg tcccgggtgc
     3121 aaccggtagc gacctgatcg ttaaaccagc tgtgaaggcg taaggagata gcaatggaat
     3181 tagtattgaa agacgcgcag agcgcgctga ctgtttccga aactaccttc ggtcgtgatt
     3241 tcaacgaagc gctggttcac caggttgttg ttgcttatgc agctggtgct cgtcagggta
     3301 ctcgtgctca gaagactcgt gctgaagtaa ctggttccgg taaaaaaccg tggcgccaga
     3361 aaggcaccgg ccgtgcgcgt tctggttcta tcaagagccc gatctggcgt tctggtggcg
     3421 tgacctttgc tgctcgtccg caggaccaca gtcaaaaagt taacaagaag atgtaccgcg
     3481 gcgcgctgaa aagcatcctg tccgaactgg tacgtcagga tcgtctgatc gttgtcgaga
     3541 agttctctgt agaagcgccg aaaactaagc tgctggcaca gaaactgaaa gacatggctc
     3601 tggaagatgt gctgatcatc accggtgagc tggacgaaaa cctgttcctg gctgcgcgca
     3661 acctgcacaa ggttgacgta cgcgatgcaa ctggtatcga cccggttagc ctgatcgcct
     3721 tcgacaaagt cgtaatgact gctgatgctg ttaagcaagt tgaggagatg ctggcatgat
     3781 tcgtgaagaa cgtctgctga aggtgctgcg tgcaccgcac gtttctgaaa aagcgtctac
     3841 tgcgatggaa aaatctaaca ccatcgtact caaagttgct aaagacgcga ccaaagcaga
     3901 aatcaaagct gctgtgcaga aactgtttga agtcgaagtc gaagtcgtta acaccctggt
     3961 agttaaaggg aaagttaaac gtcacggaca gcgtatcggt cgtcgtagcg actggaaaaa
     4021 agcttacgtc accctgaaag aaggccagaa tctggacttc gttggcggcg ctgagtaagt
     4081 cggaggagta atacaatggc agttgttaaa tgtaaaccga catctccggg tcgtcgccac
     4141 gttgttaaag tggttaaccc tgagctgcac aagggcaaac cttttgctcc gttgctggaa
     4201 aaaaacagca aatccggtgg tcgtaacaac aatggccgta tcaccactcg tcatatcggt
     4261 ggtggccaca agcaggctta ccgtattgtt gacttcaaac gcaacaaaga cggtatcccg
     4321 gcagttgttg aacgtcttga gtacgatccg aaccgttccg cgaacatcgc gctggttttg
     4381 tacaaagacg gtgaacgccg ttacatcctg gcccctaaag gcctgaaagc tggcgaccag
     4441 attcagtctg gcgttgatgc tgcaatcaaa ccaggtaaca ccctgccgat gcgcaacatc
     4501 ccggttggtt ctactgttca taacgtagaa atgaaaccag gtaaaggcgg tcagctggca
     4561 cgttccgctg gtacttacgt tcagatcgtt gctcgtgatg gtgcttatgt caccctgcgt
     4621 ctgcgttctg gtgaaatgcg taaagtagaa gcagactgcc gtgcaactct gggcgaagtt
     4681 ggcaatgctg agcatatgct gcgcgttctg ggtaaagcag gtgctgcacg ctggcgtggt
     4741 gttcgtccga ccgttcgcgg taccgcgatg aacccggtag accacccaca tggtggtggt
     4801 gaaggtcgta actttggtaa gcacccggta actccgtggg gcgttcagac caaaggtaag
     4861 aagacccgca gcaacaagcg tactgataaa ttcatcgtac gtcgccgtag caaataattt
     4921 tagaggataa gccatgccac gttctctcaa gaaaggtcct tttattgacc tgcacttgct
     4981 gaagaaggta gagaaagcgg tggaaagcgg agacaagaag cccctgcgca cttggtcccg
     5041 tcgttcaacg atctttccta acatgatcgg tttgaccatc gctgtccata atggtcgtca
     5101 gcacgttccg gtatttgtaa ccgacgaaat ggtcggtcac aaactgggtg aattcgcacc
     5161 gactcgtact tatcgcggcc acgctgctga taaaaaagcg aagaagaaat aaggtaggag
     5221 gaagagatgg aaactatcgc taaacatcgc catgctcgtt cttctgctca gaaggttcgc
     5281 cttgttgctg acctgattcg cggtaagaaa gtgtcgcagg ctctggatat tttgacctac
     5341 accaacaaga aagcggctgt actggtcaag aaagttctgg aatctgccat tgctaacgct
     5401 gaacacaacg atggcgctga cattgacgat ctgaaagtta cgaaaatttt cgtagacgaa
     5461 ggcccgagca tgaagcgcat tatgccgcgt gcaaaaggtc gtgcagatcg catcctgaag
     5521 cgcaccagcc acatcactgt ggttgtgtcc gatcgctgag actctggaga ctagcaatgg
     5581 gtcagaaagt acatcctaat ggtattcgcc tgggtattgt aaaaccatgg aactctactt
     5641 ggtttgcgaa caccaaagaa ttcgctgaca acctggacag cgattttaaa gtacgtcagt
     5701 acctgactaa ggaactggct aaagcgtccg tatctcgtat cgttatcgag cgtccggcta
     5761 agagcatccg tgtaaccatt cacactgctc gcccgggtat cgttatcggt aaaaaaggtg
     5821 aagacgtaga aaaactgcgt aaggtcgtag cggacatcgc tggcgttcct gcacagatca
     5881 acatcgccga agttcgtaag cctgaactgg acgcaaaact ggttgctgac agcatcactt
     5941 ctcagctgga acgtcgcgtt atgttccgtc gtgctatgaa gcgtgctgta cagaacgcaa
     6001 tgcgtctggg cgctaaaggt attaaagttg aagttagcgg ccgtctgggc ggcgcagaaa
     6061 tcgcacgtac cgaatggtac cgcgaaggtc gcgtaccgct gcacactctg cgtgctgaca
     6121 tcgactacaa cacctctgaa gcgcacacca cttacggtgt aatcggcgtt aaagtgtgga
     6181 tcttcaaagg cgagatcctg ggtggtatgg ctgctgttga acaaccggaa aaaccggctg
     6241 ctcagcctaa aaagcagcag cgtaaaggcc gtaaataagg agcgtcgctg atgttacaac
     6301 caaagcgtac aaaattccgt aaaatgcaca aaggccgtaa ccgcggtctg gcgcagggta
     6361 cggatgttag cttcggcagc ttcggtctga aagctgttgg ccgtggtcgt ctgactgccc
     6421 gtcagatcga agcagcacgt cgtgctatga cccgtgcagt taagcgtcaa ggtaagatct
     6481 ggatccgtgt gttcccggac aaaccgatca ctgaaaagcc gctggcagtg cgtatgggta
     6541 aaggtaaagg taacgtggag tattgggttg ccttgattca gccgggtaaa gtcctgtatg
     6601 aaatggacgg tgttccggaa gagctggccc gtgaagcatt caagctggca gcagcgaaac
     6661 tgccgattaa aaccaccttt gtaactaaga cggtgatgta atgaaagcaa aagagctgcg
     6721 tgagaagagc gttgaagagc tgaacaccga gctgctgaac ctgctgcgtg agcagttcaa
     6781 cctgcgtatg caggctgcaa gtggccagct gcaacagtct cacctgttga agcaagtgcg
     6841 tcgcgatgtc gcacgcgtta agactttact gaacgagaag gcgggtgcgt aatgaccgat
     6901 aaaatccgta ctctgcaagg tcgcgttgtt agcgacaaaa tggagaaatc cattgttgtt
     6961 gctatcgaac gttttgtgaa acacccgatc tacggtaaat tcatcaagcg tacgaccaaa
     7021 ctgcacgtac atgacgagaa caacgaatgc ggtatcggtg acgtggttga aatccgcgaa
     7081 tgccgtccgc tgtccaagac taaatcctgg acgctggttc gcgttgtaga gaaagcggtt
     7141 ctgtaataca gtacactctc tcgatacgaa taaacggctc agaaatgagc cgtttatttt
     7201 ttctacccat atccttgaag cggtgttata atgccgcgcc ctcgatatgg ggatttttaa
     7261 cgacctgatt ttcgggtctc agtagtagtt gacattagcg gagcactaaa atgatccaag
     7321 aacagactat gctgaacgtc gccgacaact ccggtgcacg tcgcgtaatg tgtatcaagg
     7381 ttctgggtgg ctcgcaccgt cgctacgcag gcgtaggcga catcatcaag atcaccatca
     7441 aagaagcaat tccgcgtggt aaggtcaaaa aaggtgatgt gctgaaggcg gtagtggtgc
     7501 gcaccaagaa gggtgttcgt cgcccggacg gttctgtcat tcgcttcgat ggtaatgctt
     7561 gtgttcttct gaacaacaac agcgagcagc ctatcggtac gcgtattttt gggccggtaa
     7621 ctcgtgagct tcgtagtgag aagttcatga aaattatctc tctggcacca gaagtactct
     7681 aaggagcgaa tcatggcagc gaaaatccgt cgtgatgacg aagttatcgt gttaaccggt
     7741 aaagataaag gtaaacgcgg taaagttaag aatgtcctgt cttccggcaa ggtcattgtt
     7801 gaaggtatca acctggttaa gaaacatcag aagccggttc cggccctgaa ccaaccgggt
     7861 ggcatcgttg aaaaagaagc cgctattcag gtttccaacg tagcaatctt caatgcggca
     7921 accggcaagg ctgaccgtgt aggctttaga ttcgaagacg gtaaaaaagt ccgtttcttc
     7981 aagtctaaca gcgaaactat caagtaattt ggagtagtac gatggcgaaa ctgcatgatt
     8041 actacaaaga cgaagtagtt aaaaaactca tgactgagtt taactacaat tctgtcatgc
     8101 aagtccctcg ggtcgagaag atcaccctga acatgggtgt tggtgaagcg atcgctgaca
     8161 aaaaactgct ggataacgca gcagcagacc tggcagcaat ctccggtcaa aaaccgctga
     8221 tcaccaaagc acgcaaatct gttgcaggct tcaaaatccg tcagggctat ccgatcggct
     8281 gtaaagtaac tctgcgtggc gaacgcatgt gggagttctt tgagcgcctg atcactattg
     8341 ctgtacctcg tatccgtgac ttccgtggcc tgtccgctaa gtctttcgac ggtcgtggta
     8401 actacagcat gggtgtccgt gagcagatca tcttcccaga aatcgactac gataaagtcg
     8461 accgcgttcg tggtttggat attaccatta ccactactgc gaaatctgac gaagaaggcc
     8521 gcgctctgct ggctgccttt gacttcccgt tccgcaagta aggtagggtt actaaatggc
     8581 taagcaatca atgaaagcac gcgaagtaaa acgcgtagct ttagctgata aatacttcgc
     8641 gaaacgcgct gaactgaaag cgatcatctc tgatgtgaac gcttccgacg aagatcgttg
     8701 gaacgctgtt ctcaagctgc agactctgcc gcgtgattcc agcccgtctc gtcagcgtaa
     8761 ccgctgccgt caaacaggtc gtccgcatgg tttcctgcgg aagttcgggt tgagccgtat
     8821 taaggtccgt gaagccgcta tgcgcggtga aatcccgggt ctgaaaaagg ctagctggta
     8881 attgtcacca attgaatcac gggagtaaag acagatgagc atgcaagatc cgatcgcgga
     8941 tatgctgacc cgtatccgta acggtcaggc cgcgaacaaa gctgcggtca ccatgccttc
     9001 ctccaagctg aaagtggcaa tcgccaacgt gctgaaggaa gaaggtttta ttgaagattt
     9061 taaagttgaa ggcgacacca agcctgaact ggaacttact ctgaagtatt tccagggcaa
     9121 agctgttgta gaaagcattc agcgtgtcag ccgcccaggt ctgcgcatct ataagcgtaa
     9181 agatgagctg ccgaaagtta tggcgggtct gggtatcgca gttgtttcta cctctaaagg
     9241 tgttatgact gatcgtgcag cgcgccaggc tggtcttggt ggcgaaatta tctgctacgt
     9301 agcctaatcg gaggaaaaaa tgtctcgtgt tgctaaagca ccggtcgttg ttcctgccgg
     9361 cgttgacgta aaaatcaacg gtcaggttat tacgatcaaa ggtaaaaacg gcgagctgac
     9421 tcgtactctc aacgatgctg ttgaagttaa acatgcagat aataccctga ccttcggtcc
     9481 gcgtgatggt tacgcagacg gttgggcaca ggctggtacc gcgcgtgccc tgctgaactc
     9541 aatggttatc ggtgttaccg aaggcttcac taagaagctg cagctggttg gtgtaggtta
     9601 ccgtgcagcg gttaaaggca atgtgattaa cctgtctctg ggtttctctc atcctgttga
     9661 ccatcagctg cctgcgggta tcactgctga atgtccgact cagactgaaa tcgtgctgaa
     9721 aggcgctgat aagcaggtga tcggccaggt tgcagcggat ctgcgcgcct accgtcgtcc
     9781 tgagccttac aaaggcaagg gtgttcgtta cgccgacgaa gtcgtgcgta ccaaagaggc
     9841 taagaagaag taaggtaaca ctatggataa gaaatctgct cgtatccgtc gtgcgacccg
     9901 cgcacgccgc aagctccagg agctgggcgc aactcgcctg gtggtacatc gtaccccgcg
     9961 tcacatttac gcacaggtaa ttgcaccgaa cggttctgaa gttctggtag ctgcttctac
    10021 tgtagaaaaa gctatcgctg aacaactgaa gtacaccggt aacaaagacg cggctgcagc
    10081 tgtgggtaaa gctgtcgctg aacgcgctct ggaaaaaggc atcaaagatg tatcctttga
    10141 ccgttccggg ttccaatatc atggtcgtgt ccaggcactg gcagatgctg cccgtgaagc
    10201 tggccttcag ttctaaggta gaggtgtaag atggctcaca tcgaaaaaca agctggcgaa
    10261 ctgcaggaaa agctgatcgc ggtaaaccgc gtatctaaaa ccgttaaagg tggtcgtatt
    10321 ttctccttca cagctctgac tgtagttggc gatggtaacg gtcgcgttgg ttttggttac
    10381 ggtaaagcgc gtgaagttcc agcagcgatc cagaaagcga tggaaaaagc ccgtcgcaat
    10441 atgattaacg tcgcgctgaa taacggtact ctgcaacacc ctgttaaagg tgttcatacg
    10501 ggttctcgcg tattcatgca gccggcttcc gaaggtaccg gtatcatcgc cggtggtgca
    10561 atgcgcgccg ttctggaagt cgctggggtt cataacgttc tggctaaagc gtatggttcc
    10621 accaacccga ttaacgtggt tcgtgcaact atcgatggcc tggaaaatat gaattcccca
    10681 gaaatggtcg ctgccaagcg tggtaaatcc gttgaagaaa ttctggggaa ataaaccatg
    10741 gcaaagacta ttaaaattac tcaaacccgc agtgcaatcg gtcgtctgcc gaaacacaag
    10801 gcaacgctgc ttggcctggg tctgcgtcgt attggtcaca ccgtagagcg cgaggatact
    10861 cctgctattc gcggtatgat caacgcggtt tccttcatgg ttaaagttga ggagtaagag
    10921 atgcgtttaa atactctgtc tccggccgaa ggctccaaaa aggcgggtaa acgcctgggt
    10981 cgtggtatcg gttctggcct cggtaaaacc ggtggtcgtg gtcacaaagg tcagaaatct
    11041 cgttctggcg gtggcgtacg tcgcggtttc gagggtggtc agatgcctct gtaccgtcgt
    11101 ctgccgaaat tcggcttcac ttctcgtaaa gcagcgatta cagccgaagt tcgtctgtct
    11161 gacctggcta aagtagaagg cggtgttgta gacctgaaca cgctgaaagc ggctaacatt
    11221 atcggtatcc agatcgagtt cgcgaaagtg atcctggctg gcgaagtcac tactccggta
    11281 actgttcgtg gcctgcgtgt tactaaaggc gctcgtgctg ctatcgaagc tgctggcggt
    11341 aaaatcgagg aataagtagc agatggctaa acaaccggga ttagattttc aaagtgccaa
    11401 aggtggctta ggcgagctga aacgcagact gctgtttgtt atcggtgcgc tgattgtgtt
    11461 ccgtattggc tcttttattc cgatccctgg tattgatgcc gctgtacttg ccaaactgct
    11521 tgagcaacag cgaggcacca tcattgaaat gtttaacatg ttctctggtg gtgctctcag
    11581 ccgtgcttct atctttgctc tggggatcat gccatatatt tcggcatcga tcattatcca
    11641 gcttctgacg gtggttcacc caacgttggc agaaattaag aaagaagggg agtctggtcg
    11701 tcgtaagatc agccagtaca cccgctacgg tactctggtg ctggcaatat tccagtcgat
    11761 cggtattgct accggtctgc cgaatatgcc tggtatgcaa ggcctggtca ttaacccggg
    11821 ctttgcattc tacttcaccg ctgttgtaag tctggtcaca ggaaccatgt tcctgatgtg
    11881 gttgggcgaa cagattactg aacgaggtat cggcaacggt atttcaatca ttatcttcgc
    11941 cggtattgtc gcgggactcc cgccagccat tgcccatact atcgagcaag cgcgtcaagg
    12001 cgacctgcac ttcctcgtgt tgctgttggt tgcagtatta gtatttgcag tgacgttctt
    12061 tgttgtattt gttgagcgtg gtcaacgccg cattgtggta aactacgcga aacgtcagca
    12121 aggtcgtcgt gtctatgctg cacagagcac acatttaccg ctgaaagtga atatggcggg
    12181 ggtaatcccg gcaatcttcg cttccagtat tattctgttc ccggcgacca tcgcgtcatg
    12241 gttcgggggc ggtactggtt ggaactggct gacaacaatt tcgctgtatt tgcagcctgg
    12301 gcaaccgctt tatgtgttac tctatgcgtc tgcaatcatc ttcttctgtt tcttctacac
    12361 ggcgttggtt ttcaacccgc gtgaaacagc agataacctg aagaagtccg gtgcatttgt
    12421 accaggaatt cgtccgggag agcaaacggc gaagtatatc gataaagtaa tgacccgcct
    12481 gaccctggtt ggtgcgctgt acattacctt tatctgcctg atcccggagt tcatgcgtga
    12541 tgcaatgaaa gtaccgttct actttggtgg gacctcactg cttatcgttg ttgtcgtgat
    12601 tatggacttt atggctcaag tgcaaactct gatgatgtcc agtcagtatg agtctgcatt
    12661 gaagaaggcg aacctgaaag gctacggccg ataattggtc gcccgagaag ttacggagag
    12721 taaaaatgaa agttcgtgct tccgtcaaga aattatgccg taactgcaaa atcgttaagc
    12781 gtgatggtgt catccgtgtg atttgcagtg ccgagccgaa gcataaacag cgccaaggct
    12841 gattttttcg catatttttc ttgcaaagtt gggttgagct ggctagatta gccagccaat
    12901 cttttgtatg tctgtgcgtt tccatttgag tatcctgaaa acgggctttt cagcatggaa
    12961 cgtacatatt aaatagtagg agtgcatagt ggcccgtata gcaggcatta acattcctga
    13021 tcataagcat gccgtaatcg cattaacttc gatttatggc gtcggcaaga cccgttctaa
    13081 agccatcctg gctgcagcgg gtatcgctga agatgttaag atcagtgagc tgtctgaagg
    13141 acaaatcgac acgctgcgtg acgaagttgc caaatttgtc gttgaaggtg atctgcgccg
    13201 tgaaatcagc atgagcatca agcgcctgat ggatcttggt tgctatcgcg gtttgcgtca
    13261 tcgtcgtggt ctcccggttc gcggtcagcg taccaagacc aacgcacgta cccgtaaggg
    13321 tccgcgcaaa ccgatcaaga aataatcggg gtgattgaat aatggcaaag gcaccaattc
    13381 gtgcacgtaa acgtgtaaga aaacaagtct ctgacggcgt ggctcatatc catgcttctt
    13441 tcaacaacac catcgtgact atcactgatc gtcagggtaa cgcgttgggt tgggcaacag
    13501 ccggtggttc cggtttccgt ggttctcgca aatccactcc gtttgcagct caggttgcag
    13561 cagagcgttg cgctgacgcc gtgaaagaat acggcatcaa gaatctggaa gttatggtta
    13621 aaggtccggg tccaggccgc gaatctacta ttcgtgctct gaacgccgca ggtttccgca
    13681 tcactaacat tactgatgtg actccgatcc ctcataacgg ttgtcgtccg ccgaaaaaac
    13741 gtcgcgtata acgcttcgtt ttccaggttt gttggagaaa gaaaatggca agatatttgg
    13801 gtcctaagct caagctgagc cgtcgtgagg gcaccgactt attccttaag tctggcgttc
    13861 gcgcgatcga taccaagtgt aaaattgaac aagctcctgg ccagcacggt gcgcgtaaac
    13921 cgcgtctgtc tgactatggt gtgcagttgc gtgaaaagca aaaagttcgc cgtatctatg
    13981 gtgtgctgga gcgtcagttc cgtaactact acaaagaagc agcacgtctg aaaggcaaca
    14041 ccggtgaaaa cctgttggct ctgctggaag gtcgtctgga caacgttgta taccgtatgg
    14101 gcttcggtgc cactcgtgca gaagcacgtc agctggttag ccataaagca attatggtaa
    14161 acggtcgtgt tgttaacatc gcttcttatc aggttagtcc gaatgacgtt gtaagcattc
    14221 gtgagaaagc gaagaagcag tctcgcgtga aagccgctct ggagctggct gagcagcgtg
    14281 aaaagccaac ctggctggaa gttgatgctg gcaagatgga aggtacgttt aagcgtaagc
    14341 cggagcgttc tgatctgtct gcggacatta acgaacacct gatcgtcgag ctttactcca
    14401 agtaaagctt agtaccaaag agaggacaca atgcagggtt ctgtgacaga gtttctaaaa
    14461 ccgcgcctgg ttgatatcga gcaagtgagt tcgacgcacg ccaaggtgac ccttgagcct
    14521 ttagagcgtg gctttggcca tactctgggt aacgcactgc gccgtattct gctctcatcg
    14581 atgccgggtt gcgcggtgac cgaggttgag attgatggtg tactacatga gtacagcacc
    14641 aaagaaggcg ttcaggaaga tatcctggaa atcctgctca acctgaaagg gctggcggtg
    14701 agagttcagg gcaaagatga agttattctt accttgaata aatctggcat tggccctgtg
    14761 actgcagccg atatcaccca cgacggtgat gtcgaaatcg tcaagccgca gcacgtgatc
    14821 tgccacctga ccgatgagaa cgcgtctatt agcatgcgta tcaaagttca gcgcggtcgt
    14881 ggttatgtgc cggcttctac ccgaattcat tcggaagaag atgagcgccc aatcggccgt
    14941 ctgctggtcg acgcatgcta cagccctgtg gagcgtattg cctacaatgt tgaagcagcg
    15001 cgtgtagaac agcgtaccga cctggacaag ctggtcatcg aaatggaaac caacggcaca
    15061 atcgatcctg aagaggcgat tcgtcgtgcg gcaaccattc tggctgaaca actggaagct
    15121 ttcgttgact tacgtgatgt acgtcagcct gaagtgaaag aagagaaacc agagttcgat
    15181 ccgatcctgc tgcgccctgt tgacgatctg gaattgactg tccgctctgc taactgcctt
    15241 aaagcagaag ctatccacta tatcggtgat ctggtacagc gtaccgaggt tgagctcctt
    15301 aaaacgccta accttggtaa aaaatctctt actgagatta aagacgtgct ggcttcccgt
    15361 ggactgtctc tgggcatgcg cctggaaaac tggccaccgg caagcatcgc tgacgagtaa
    15421 ccggatcaca ggttaaggtt ttactgagaa ggataaggtc atgcgccatc gtaagagtgg
    15481 tcgtcaactg aaccgcaaca gcagccatcg ccaggctatg ttccgcaata tggcaggttc
    15541 actggttcgt catgaaatca tcaagacgac tctgcctaaa gcgaaagagc tgcgccgcgt
    15601 agttgagccg ctgattactc ttgccaagac tgatagcgtt gctaatcgtc gtctggcatt
    15661 cgcccgtact cgtgataacg agatcgtggc aaaactgttt aacgaactgg gcccgcgttt
    15721 cgcgagccgt gccggtggtt acactcgtat tctgaagtgt ggcttccgtg caggcgacaa
    15781 cgcgccgatg gcttacatcg agctggttga tcgttcagag aaagcagaag ctgctgcaga
    15841 gtaatctgaa gcaacgtaaa aaaacccgcc ccggcgggtt tttttatacc cgtagcatcc
    15901 ccacttatct acaatagctg tactcttttt gttcatcccc tggagtatat atgtggttac
    15961 ttgaccagtg ggcagagcgt catatagcag aagcgcaagc gaaaggtgag tttgataacc
    16021 tggcaggtag cggcgaacca ttgatactgg ctgatgattc tcatgtgcca ccggaattac
    16081 gtgcggggta tcgcttgctg aagaatgccg gttgcttact gccagaactt gagcagcgga
    16141 gggaagcaat tcagcttctg gatatcctga gaggtatccg tcacgatgat ccgcaatatc
    16201 aagaggttag ccgtcgattg tcattactgg aattgaagct gcgacaagct ggactgagca
    16261 ccgatttttt acgcggcgat tatgctgaca agttgttgaa taaaatcaac gataactagt
    16321 ggagtatgta tgtatcgcat tggtgagctg gcaaaaatgg cggaagtaac acccgacacg
    16381 attcgttatt acgaaaaaca gcagatgatg gagcatgaag tgcgtactga aggtgggttt
    16441 cgcctgtata ccgaaagcga tctccagcga ttgaaattta tccgccatgc cagacaacta
    16501 ggtttcagtc tagagtcgat ccgcgagttg ctgtcgatcc gtatcgatcc tgaacaccat
    16561 acctgtcagg agtcaaaagg cattgtgcag gaaagattgc aggaagtcga agcacggata
    16621 gccgagttgc agagtatgca gcgttccctc caacgcctta acgatgcctg ttgtgggacc
    16681 gcccatagca gtgtttattg ctcgattctt gaagctcttg aacaaggggc gagtggcgtt
    16741 aagagtggct gttgattttt tacactggca ggattacact cgcgccgtta aataaccaat
    16801 tggagttttt atgagccgat atcagcatac taaagggcag ataaaggata atgcgataga
    16861 agcattacta catgatccct tattccgaca gcgcgtagag agaaataaga aagggaaagg
    16921 cagttacatg cgaaaaggaa aacatggcaa tcgggggaac tgggaggcca gtggcaagaa
    16981 agtaaatcac ttttttacca ctggccttct gctttcaggc gcttgttaag agcggttatt
    17041 ctgctctttc agcaaatcac gaatttctgt cagtaatact tcttctttag ttggtgtagg
    17101 cgcggctgct ggttcttctt ttttccgatt cagtttgttg attagcttaa tcgccataaa
    17161 gatggcaaag gctacaatca gaaaatcaaa gacgttttga atgaagacac cgtaatgcat
    17221 cacaacagca gggatatccc cctgcgcatc gcgtagcgtg acagcaaact gtttgaaatc
    17281 gatcccgcca attaataacc ccagaggtgg cataatgata tcggcaacca gtgaagatac
    17341 aatcttcccg aatgccgcac cgataatgac acccaccgcc aaatccacca cgttcccgcg
    17401 catcgcaaat tcgcgaaatt ctttaataat gctcatgtta ttctccctat gaagccgaca
    17461 atcataagtc taacaaatgt taagctattt tcctgccagg agtgaattta attaatccct
    17521 gataatcaaa gggaaataat aaggcgtcat tagacgcctt attaattaca agaagaaagg
    17581 acttggctgg aagagtcttt cgacgtcggt aataaacttt ttatctgtga ggaacataat
    17641 tacgtgatcg ccttgctcaa tgcgcagatt gtcattggca atcatcacgt cgtttccacg
    17701 taccaccgct ccaataatcg ttcctggcgg tagcttgatt tcatcaatga ctctgccgac
    17761 aacgcgtgag gtgctttcat caccgtgagc aacggcttca atagcttctg ctacgccgcg
    17821 gcgcagtgag gaaacaccaa caatatcagc ttttcgcaca tggctaagca acgcagaaat
    17881 agttgcttgt tgcggtgaaa tcgcaatatc gatcacgctc ccctgaacca gatccacata
    17941 agcgcgacgc tggatcaata ccatcacctt tttcgcaccc atacgtttgg caagcatggc
    18001 ggacatgata ttggcttcgt catcgttggt gacagcaata aacagatcaa cttgatcgat
    18061 atgttcttcg gccagcagtt cttgatccga cgcatcacca aaaaagacga tcgtattctg
    18121 caacttttcc gccagttcgg cagcgcgctg ctgattacgt tcgatgagtt taacgctgta
    18181 atctttttcc agacgacgcg ccagccctgc accgatatta ccgccgccaa ccagcatgat
    18241 ccgcttatac ggtttttcca gtcgctgtaa ttcactcatc accgcgcgga tatgctgtga
    18301 agcggcaata aagaacactt catcaccagc ttcaacaatg gtcgaacctt gcggacgaat
    18361 ggggcgatcg tggcggaaaa ttgctgccac acgagtatcg atatggggca tatgttcgcg
    18421 catggtcgaa agtgcattac caatcagcgg gccgccataa taggctttaa ccacagccag
    18481 gctgacttta ccctcagcga agttcaccac ctgcaatgcg ccgggatact caatcagtcg
    18541 gtaaatatta tcgataacca actgctctgg tgcgatcaga tgatcaatcg gtacagcatc
    18601 tgaatgaaat agtttatcgg catcgcgcac gtagtctggt gagcggatac gagcgatgcg
    18661 attaggggtg ttgaaaagtg agtaggctac ctggcaggca accatattgg tttcatctga
    18721 actagttaca gcaaccagca tatcggcgtc gtcggcacct gcctcccgca atacgcgtgg
    18781 atgagagcca tgcccctgca cgactcgcag gtcaaattta tcctgcaagg tccgcagacg
    18841 ctcaccgttg gtatcgacaa cagtaatatc gttgttctcg ccaaccaggt tttccgccag
    18901 tgtgccgcca acctggccgg cacccagaat gataattttc atcagtcgcg acccgttatc
    18961 tcatcacttt ttgattagct tagcgtaaaa gaagccgtcg ccctcttcgg caccaggcag
    19021 attttgttta cccggttgct ctggtgttcc tgtttcgcaa agttcggcat cagcggtacg
    19081 ttgcaaaaag gctttaatct gcaggctatt ctcttccggt aacaccgaac aggtggcata
    19141 gaccagggtt ccaccggatt ttaaatgcgg ccaaatggcg tcgagaattt cagactgcaa
    19201 ttgcgcgagt tccgggatat cgcgatcgcg acgtaaccat ttaatatccg gatggcgacg
    19261 aatcacaccg gttgctgaac aaggcgcatc taataaaatg cgatcaaact gttgctcgcc
    19321 acaccattgg gaagggtagc ggccatcacc ttgtttcacg gttgccttca taccaaggcg
    19381 ttttaaattg tcgtaaacgc gagagaggcg ctgttcgtca atatcaaccg caacaacctg
    19441 cgcttctggt gccacctcaa ggatatgcgt tgttttaccg ccgggggcgg cacaaagatc
    19501 caaaatgtgt tcaccgtttt gtggcgcaag ccaggtcatg caaccttgtg ctgaggcatc
    19561 ctgaacggta acccatccgt cttcaaaacc aggtagcgca tgaacaggtg caggtgtttc
    19621 cagacgtaca gcatcagggt aatccgcagg cgggaaacct ttcattcctg cttcatccag
    19681 caatgcaagc cagctgtcgc gggaatgatg cgtacgatta acgcgcagcc acattggcgg
    19741 acgctggtta ttggcttcga cgatggattg ccactgctct ggatacgctt tttgcagacg
    19801 cttcagcaac caggaaggat gcagataacg tgcatcactg gcattaaact cggctaataa
    19861 ctcttcttgc tgacgctgga actggcgtaa tacaccgttt atcaaccctt taagttgcgg
    19921 gcgcttaatt gcgatagcgc cttcaaccgt ttcagccagc gcagcatgag gtggtatgcg
    19981 ggtataaagc agttgataca aaccaaccat aatcaggtaa tgcacggtcc gctgtttgcc
    20041 ggtcatcgga cgggccatta acttattaat cagccagttt aactgcgaaa gcgtacgcag
    20101 tacgccaaag cacaactctt gaagaagtgc tttgtcttta tcggaaactt tttgctggag
    20161 cggtggcaga atgttgctta atgattgccc ttgctcgacg acttgttcaa cggcctgggc
    20221 cgccatgcta cgtaaattac gttgtttttt cataagtata aaaatgcccg gcaagaccgg
    20281 gcttagaaga gtggactatc agaccagacg gttgcccgga acaaaccatt cccgacgaga
    20341 gttcaggagg tcttgcgcgc tcatcgcttt cttacccgca ggttgtaacg agagcaggtt
    20401 caggatgcca tcaccagtcg caacctgaat gccttgtttg ttggcttcaa ggatcgttcc
    20461 tggtgcagcg ttggttgccg tatcaatgac cgatgctttc cagactttaa ccggctgtcc
    20521 ttcaatttcc agccagctca ttggccatgg attgaaagcg cgaatgcagc gttcaagctg
    20581 tgctgccgaa agtgaccagt caatacgcgc ttcttcttta ctcaacttct cggcgtaagt
    20641 gacaagagtt tcgtcctgaa cttctggttt cgccgtgccg tctgccaatt gtttcaacgt
    20701 ggtgataagc ccttgtgggc caagctctgc cagcttgtcg tacagcgtac cactggtatc
    20761 ttctgcagta atcgggcagg agagcttata gagcatgtct ccggtgtcta aaccaacatc
    20821 catttgcata atggtcacac cagtttctgc atcacccgcc catagtgagc gttggattgg
    20881 tgcagcaccg cgccagcgtg gcagcagtga accatgaacg ttgatacagc caagacgcgg
    20941 catctccagc actgctttag gcagaattaa gccataggcg acgacaacca taacatcagc
    21001 ctgtagatcg gcgaccagtt gctggttttc ttgtggacgc agggaaaccg gttgaaaaac
    21061 gggcagacct ttttcctcag ccagaacttt aaccgggctg ggcatcagtt ttttaccgcg
    21121 tcctgccggt cggtctggct gggtgaacac gccaacgacg ttatgaccag aagacaacag
    21181 cgcgtcgaga tgacgcgctg caaagtcagg tgtacccgca aaaataatac gtagtgattc
    21241 tgacacgtta gttcttatcc ttaagcccgg gctttcagac gatccagttt ttcaactttc
    21301 tgacgaatac gttgttgttt cagcggtgac agataatcca taaacagttt gccgaccagg
    21361 tgatccatct catgctgaat acagatggct aacagaccct ctgcttccag ttcaaatggt
    21421 ttaccgtcgc ggtccagggc gcgaatttta actttctctg cgcgtggcac taaagcacgt
    21481 tgttcaggga tcgacaggca tccttcttca atgcctgttt cgccgctttt ttctaaaagc
    21541 tccggattga ttaacaccag ccgttcgtca cggttttccg aaacatcaat aacaatgata
    21601 cgttgatgga tatcgacctg ggttgccgcc aggccaatac cttcttctgc gtacatcgtc
    21661 tcgaacatat catcgacgat acgctgaatt tctgcattca cttcttctac cggtttagca
    21721 actttgcgaa gccgctcgtc cggaatatgt aacacttgca aaactgacat aaatctccag
    21781 agatgtgttc aggagttaga aagattattt cttctattct agacaaatcc ccctctgatt
    21841 gacagcatca ctgaccaatc gcaaagattg ctaaggctgc ttatggcagg gagataagga
    21901 tggtcgatac agaaatttgg ctgcgtttaa tgagcatcag cagcttgtac ggcgatgata
    21961 tggtccgtat agctcactgg ctggcaaaac agtcgcatat tgatgcggtt gtattgcagc
    22021 aaactgggct tacattgcgg caggcacaac gctttctttc atttccgcga aagagtatcg
    22081 aaagctcact ttcttggttg gagcaaccca accatcattt aattcctgcg gacagcgaat
    22141 tttatcctcc tcaacttctt gcgacgacag attaccccgg cgcactgttt gttgaaggag
    22201 aactgcacgc gctgcattca tttcagcttg ccgtagtggg gagtcgggcg cattcatggt
    22261 atggcgagcg atggggacga ttattttgcg aaactctggc gacgcgtgga gtgacaatta
    22321 cgagtggact ggcgcgtgga atcgatggtg tggcgcataa agcggcctta caggtaaatg
    22381 gcgtcagcat tgctgtattg gggaatggac ttaataccat tcatccgcgt cgccatgccc
    22441 gactggctac cagtttgctt gaacatggtg gggctcttgt ctcggaattt cccctcgatg
    22501 ttccacccct tgcttacaat ttcccacgaa gaaatcgcat tatcagtggt ctaagtaaag
    22561 gtgtactggt ggtggaagcg gctttgcgca gtggttcgct ggtgacagca cgttgtgcgc
    22621 ttgagcaggg gcgtgaagtt tttgccttgc cgggtccatt agggaatccg ggaagcgaag
    22681 ggcctcactg gttaataaaa caaggtgcga ttcttgtgac ggaaccggaa gaaattctgg
    22741 aaaacttgca atttggattg cactggttgc cagacgcccc tgaaaattca ttttattcac
    22801 cagatcagga agacgtggca ttgccatttc ctgagctcct ggctaacgta ggagatgagg
    22861 taacacctgt tgacgtcgtc gctgaacgtg ccggccaacc tgtgccagag gtagtaactc
    22921 aactactcga actggagtta gcaggatgga tcgcagctgt acccggcggc tatgtccgat
    22981 tgaggagggc atgccatgtt cgacgtacta atgtatttgt ttgaaaccta tattcacaca
    23041 gaagctgagt tgcgtgtgga tcaagacaaa cttgaacagg atcttaccga cgcaggattt
    23101 gatcgagaag atatctacaa tgccctgctt tggctggaaa agcttgcgga ttatcaggaa
    23161 ggactggcag aaccgatgca actggcctct gatccactct ccatgcgtat ttatacccct
    23221 gaagagtgtg aaaggctgga tgccagctgt cgtggttttc tgcttttcct tgagcaaatt
    23281 caggtgctta accttgaaac ccgtgaaatg gtgatagagc gagtgctcgc gctggatacc
    23341 gcagagttcg acctggaaga cctgaaatgg gtaatcctga tggtgttgtt caatattccg
    23401 ggctgcgaaa atgcgtacca gcaaatggaa gaattactct ttgaagtgaa tgaaggtatg
    23461 ctgcattaat catttcttta attcagcata agttgttatg gcgaaatcag cactgttcac
    23521 ggtgcgtaat aatgagtcct gcccaaagtg cggggctgaa ctggttattc gatccgggaa
    23581 acacggtccg tttcttggat gctcacagta tccggcgtgt gactacgtcc gtcctctgaa
    23641 atcttcagcg gatggacata ttgtcaaagt tctggagggg caggtttgcc ctgcatgtgg
    23701 cgcaaatctg gtattacgcc agggacgctt tggtatgttt attggttgca gtaactaccc
    23761 tgaatgcgaa cataccgaac ttatcgataa accggacgaa acagcaatta cctgtcccca
    23821 atgtcggacg ggccatctgg tccagcgccg ttcccgttat ggcaaaacat ttcactcctg
    23881 tgatcgctac ccggagtgtc aatttgccat taacttcaaa cccatagctg gagaatgccc
    23941 tgagtgtcat tatccgctac tcatcgaaaa gaaaaccgcg cagggtgtaa aacacttttg
    24001 tgccagtaaa caatgtggaa agccggtttc ggcggaataa taacgtgaat aataacctgc
    24061 aaggagacgc tatcgcagct gcgatagatg tcctcaatga aaaacgtgtc atcgcctatc
    24121 caacggaagc cgttttcggt gtcgggtgcg atcctgatag cgaaacagca gtgatgcgac
    24181 tgttggagtt aaaacagcgt ccggttgata aggggctgat tttaatcgcg gcaaattacg
    24241 agcagcttaa accctatatt gatgacacca tgctgactga tgcgcagcgt gaaaccattt
    24301 tttcccgctg gccaggtcct gtcacctttg tctttcccgc gcctgcgaca acaccgcgct
    24361 ggttgacggg ccgctttgat tcgcttgctg tacgagtcac cgaccatccg ttggtggttg
    24421 ctttgtgcca ggcttatggt aaaccgctgg tttctaccag tgccaacttg agtggattgc
    24481 caccttgtcg aacagtagac gaagttcgcg cacaatttgg cgcggcgttc ccggttgtgc
    24541 ctggtgaaac gggggggcgt ttaaatcctt cagaaatccg cgatgccctg acgggtgaac
    24601 tgtttcgaca ggggtaacat aatggaaacc tatgctgttt ttggtaatcc gatagcccac
    24661 agcaaatcgc cattcattca tcagcaattt gctcagcaac tgaatattga acatccctat
    24721 gggcgcgtgt tggcacccat caatgatttc atcaacacac tgaacgcttt ctttagtgct
    24781 ggtggtaaag gtgcgaatgt gacggtacct tttaaagaag aggcttttgc cagagcggat
    24841 gagcttactg aacgggcagc gttggctggt gctgttaata ccctcaagcg gttagaagat
    24901 ggacgcctgc tgggtgacaa taccgatggt ataggtttgt taagcgatct ggaacgtctg
    24961 tcttttatcc gtcctggttt acgtattctg ctcatcggcg ctggtggagc ttctcgcggc
    25021 gtactactgc cacttctttc cctggactgt gcggtgacaa tcactaatcg gacggtatcc
    25081 cgcgcggaag agttggctaa attgtttgcg cacactggca gtattcaggc gttgggtatg
    25141 gacgaactgg aaggtcatga gtttgatctc attattaatg caacatccag tggcatcagt
    25201 ggtgatattc cggcgatccc gtcatcgctt attcatccag gcatttattg ctatgacatg
    25261 ttctatcaga aaggaaaaac tccttttctg gcatggtgtg agcagcgagg ctcaaagcgt
    25321 aatgctgatg gtttaggaat gctggtggca caggcggctc atgccttcct tctttggcat
    25381 ggcgttctgc ctgatgtaga accggtcatc aaactattgc aacaggaatt gtcagcgtga
    25441 atcaggccat ccagtttccg gatagggaag agtgggacga gaataaaaga tgtgtatgtt
    25501 ttcccgctct cgtgaatggt atgcaactga catgtgcgat ctctggcgag agtctggcgt
    25561 atcgctttac tggagatacg ccagaacagt ggttagcgag ttttcgtcag catcgctggg
    25621 acctggaaga agaagcggaa aacttaattc aggaacaaag tgaagatgat caaggttggg
    25681 tctggttacc ctgatccaga tattcgtcct tccatttcac gtaattcttc gcggaatagc
    25741 gtaacccagc cttctcttca tcacttaacg ggcggatctg tttgacgggg ctaccgagat
    25801 acagatatcc gctctccagc cgtttatttt gtgggaccag gctacccgca ccaatcatca
    25861 catcatcttc tactattgcg ccatcaagta aaattgagcc catcccaacc aaaactcgat
    25921 tgccaatggt gcagccgtgg agcatcacct tgtgaccaac agtgacatct tcgccaatgg
    25981 ttaatgggtt gccatctggg ttgtacgagg atttatgagt gacatgcaac atactgccat
    26041 cctggatatt ggtgcgtgct ccgatctgta cataatgtac atctccacga atcacaacga
    26101 gcggccagat ccccacatca tcagccagac gaacgtcacc aatcacgacg ctgctatcgt
    26161 cgatcattac gcgctggccg atttgtggaa aaagatcgcg gtatgggcgt aaaacatcag
    26221 acatactcac ctcaacaata aatgatttac taatgacttt gggtgcatta ttggccttgt
    26281 gcaagtcttt tagcatgcaa aaaagcaccg ttttgtgtgc gaatgcagca aaaagggtga
    26341 aaaagcaaca aacagaaaaa aagatcaaaa aagtacttgt gcaaaaaatt gggatcccta
    26401 taatgcgcct ccgttgagac gacaacgtga aacacttcac aagatggtcg taacaacaaa
    26461 gagaaaaaat cctgaaattc
//
LOCUS       DAZGQL010000075        25986 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.075, whole genome shotgun sequence.
ACCESSION   DAZGQL010000075 DAZGQL010000000
VERSION     DAZGQL010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25986
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.075"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(29..4489)
                     /gene="mukB"
                     /locus_tag="ACHKIR_003480"
     CDS             complement(29..4489)
                     /gene="mukB"
                     /locus_tag="ACHKIR_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HFO9991166.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPYQAS"
     gene            complement(4489..5193)
                     /gene="mukE"
                     /locus_tag="ACHKIR_003481"
     CDS             complement(4489..5193)
                     /gene="mukE"
                     /locus_tag="ACHKIR_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HFO9991167.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEESQPDSGEEE"
     gene            complement(5174..6496)
                     /gene="mukF"
                     /locus_tag="ACHKIR_003482"
     CDS             complement(5174..6496)
                     /gene="mukF"
                     /locus_tag="ACHKIR_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HFO9991168.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHCHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMVLR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6493..7278)
                     /gene="cmoM"
                     /locus_tag="ACHKIR_003483"
     CDS             complement(6493..7278)
                     /gene="cmoM"
                     /locus_tag="ACHKIR_003483"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HFO9991169.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7414..8193
                     /gene="elyC"
                     /locus_tag="ACHKIR_003484"
     CDS             7414..8193
                     /gene="elyC"
                     /locus_tag="ACHKIR_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HFO9991170.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKSSSGEPRQE"
     gene            complement(8170..9063)
                     /locus_tag="ACHKIR_003485"
     CDS             complement(8170..9063)
                     /locus_tag="ACHKIR_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HFO9991171.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDTSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9217..9963)
                     /gene="kdsB"
                     /locus_tag="ACHKIR_003486"
     CDS             complement(9217..9963)
                     /gene="kdsB"
                     /locus_tag="ACHKIR_003486"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFO9991172.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAKGLETVGDNFLRHLGIYGYRAGFIRRYVNWQPSPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(9960..10142)
                     /gene="ycaR"
                     /locus_tag="ACHKIR_003487"
     CDS             complement(9960..10142)
                     /gene="ycaR"
                     /locus_tag="ACHKIR_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HFO9991173.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10194..11426)
                     /gene="ycaQ"
                     /locus_tag="ACHKIR_003488"
     CDS             complement(10194..11426)
                     /gene="ycaQ"
                     /locus_tag="ACHKIR_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ"
                     /protein_id="HFO9991174.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLGDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHAAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPEWNDERDLVSQAEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTTLQKGLRQAITDFANWQQATRVTLGRC
                     PQGLFTDCRAGWEIDPVA"
     gene            complement(11463..12449)
                     /gene="lpxK"
                     /locus_tag="ACHKIR_003489"
     CDS             complement(11463..12449)
                     /gene="lpxK"
                     /locus_tag="ACHKIR_003489"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HFO9991175.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTGAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     NAGQTLVMTEKDAVKCRAFAEGNWWYLPVDAQLSGDEPAKLLAQLTSLASGN"
     gene            complement(12446..14194)
                     /gene="msbA"
                     /locus_tag="ACHKIR_003490"
     CDS             complement(12446..14194)
                     /gene="msbA"
                     /locus_tag="ACHKIR_003490"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0034040 - ATPase-coupled lipid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HFO9991176.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLMWMPLVVIGLMILRGITSYISSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14231..16495)
                     /locus_tag="ACHKIR_003491"
     CDS             complement(14231..16495)
                     /locus_tag="ACHKIR_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HFO9991177.1"
                     /translation="MKITTVGVCIICGIFPLLILPQLPGTLTLASLTVFACVLVFIPF
                     KTVRYIALTLLFFVWGILAAKQILWAGETLTGATQDAIVEITATDGMTTHYGQITHLQ
                     GRRIFPAPGLVLYGEYLPQAVCAGQLWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASVIEPNCSLRAQYLASLQTTLQPYMWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAALLAAGLIRGGQVFLPGRWIHWQMPLIGGICCAAFYAWLTG
                     MQPPALRTVVALAMWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     FAIPLVTFITVPLILAAMVAHLSGPFILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ERWQWLTFSPWFLLVVWRLNAWRTLPAMCVAVGLLMCLPLWQKPRPDEWQVYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHTWPMLWIRSQLNWEHHQPCVRGEAWQWQGLRFSAHWPLQGSNDKGNNHSCVVK
                     IDDGTNSILLTGDIEAPAEQKMLSRYWQQMQATLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSSKVKHRYQQQGYKWLDTPHQGQITVNFSAQGWRISSLREQILP
                     RWYHQWFGVPVDNG"
     gene            complement(16702..16986)
                     /gene="ihfB"
                     /locus_tag="ACHKIR_003492"
     CDS             complement(16702..16986)
                     /gene="ihfB"
                     /locus_tag="ACHKIR_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HFO9991178.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(17146..18819)
                     /gene="rpsA"
                     /locus_tag="ACHKIR_003493"
     CDS             complement(17146..18819)
                     /gene="rpsA"
                     /locus_tag="ACHKIR_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFO9991179.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFIGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(18930..19613)
                     /gene="cmk"
                     /locus_tag="ACHKIR_003494"
     CDS             complement(18930..19613)
                     /gene="cmk"
                     /locus_tag="ACHKIR_003494"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFO9991180.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            complement(19786..20550)
                     /gene="ycaL"
                     /locus_tag="ACHKIR_003495"
     CDS             complement(19786..20550)
                     /gene="ycaL"
                     /locus_tag="ACHKIR_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HFO9991181.1"
                     /translation="MKNTKLLLAIAASAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
     gene            complement(20719..22002)
                     /gene="aroA"
                     /locus_tag="ACHKIR_003496"
     CDS             complement(20719..22002)
                     /gene="aroA"
                     /locus_tag="ACHKIR_003496"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFO9991182.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQDNYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNMMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRIAMCFS
                     LVALSDTPVTILDPKCTAKTFPNYFEQLARISQAA"
     gene            complement(22073..23161)
                     /gene="serC"
                     /locus_tag="ACHKIR_003497"
     CDS             complement(22073..23161)
                     /gene="serC"
                     /locus_tag="ACHKIR_003497"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HFO9991183.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNDSMFNTPPTFAWYLSGLVFKWLKANGGVAAMDKINQQKAELL
                     YGVIDNSNFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(23360..24052)
                     /locus_tag="ACHKIR_003498"
     CDS             complement(23360..24052)
                     /locus_tag="ACHKIR_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFO9991184.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFIILALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESVDYACIRCSEIIHMNAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            24182..25942
                     /gene="ycaO"
                     /locus_tag="ACHKIR_003499"
     CDS             24182..25942
                     /gene="ycaO"
                     /locus_tag="ACHKIR_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory factor YcaO"
                     /protein_id="HFO9991185.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLSENDDVPEGLLDDRLRAFYDPENELAGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
BASE COUNT         6303 a         7078 c         6319 g         6286 t
ORIGIN      
        1 aaaaggcggc attgctgccg ccttcatttt aactcgcttg gtaaggcgct tcgtcacttc
       61 ctggaagcgt ttcagggagt tgcggcgcaa atcctcgcag gccgacgacg tgaacgtgtt
      121 cggtattctg gaagacttta cgcaccagtt tataggtggt gcctttttcc ggactgatat
      181 tttccggcgc tgcgatgatg agctgcattt gcaaacgttc acacaattca aacagcgtgg
      241 cgatagaacg agcatccagt cgcgctgctt catcgaggaa cagcaggcgg caaggagaga
      301 tatctttacc gcgcaggcgg cgagattcat cttcccagct ttgtaccacc atcaccagaa
      361 tcgacatacc ggtaccaatc gcctcaccgg tcgacagagc gccagattct gcgcgcagcc
      421 agccatcaga accacggtta acctcaactt ccatttccag atagttgcgg taatccagca
      481 gttcttcgcc aatggtctgc ggcgtgcgct gccccatatc aatctgcgga ttaagacgct
      541 gatacaattt cgccagcgct tccgagaagg tcagacggtt gctgttaaac agatcctgat
      601 gctgctcgtg ctgttcagag agcacatcca gcagcatggc gtgcgtttca cgcacgttca
      661 cattcagacg tacgctgttt acctgaccga acgatacgtt ctgcaaaccc tggttgagca
      721 tacggatacg gttctgttcg cgctgaatgg ttttgcgaat aatgttcgcc acgctgcggg
      781 aactgatcgc cagtttctgt tcacgggagg ttaactcttc ggtcagacgg ctaagttcga
      841 tctccatctg ttcgatagct tccaccggat catcggtacg aataatatcc tgacgaatac
      901 gttcacgcag atgctgataa accgccacga agaactgaat tttacgttcc ggacgttttg
      961 gatcttccga catccgcagc acgtcgcgca gatgttcgtt atccgccacc gccagacgca
     1021 gcgcacctaa cgccttatcc gacatggaac gcaaatcatc agcggagaga taagccagct
     1081 cacgacggtg taagcggcgt tcaacgccgt tatctttcac catgcgcatg actgcacacc
     1141 agcccgcttt cgcggtcact acctgctcgc gcatctcaaa gtaatcacgc tccagcttgc
     1201 gcagtttgcg ggtcaggttg tccatctccg cttcgcagaa ggtaagcgct ttttccagtt
     1261 gattgcggcg tgaacggtta ttgctcagtt gcgcgtgcaa ctcgtcacgg cgaatacgcg
     1321 cccgctcttc tgccccgcta tcagcacgca cgccgatatc ctgcaattca cgttgcagat
     1381 cgttgagtag ctcttttttg gtgtcgtaag aacttttcag cgaagccagc acctggttgt
     1441 actgactcaa ctgcgctgcg tgtccgcgca acgcttcacg cgcgcgggta cgctccgctt
     1501 ccgcctgttc cagacgttca cgcagttttt cgttgagatc gctgttaccg ctcagcattt
     1561 ctgccgagtc agagtagcta aagtgcgcac gacgctgcac cacttccgtc agggcaaacg
     1621 cctgctgacg ggcatcgcgc tgcatctgct gagagtacgc gtaatcttct tttaactgtt
     1681 cgaactgttc cgggtcgctc tgcaataccg aaacgatcgg ttccagtttc gccagttgat
     1741 tgccaaactg ctgaacaaaa cgcgcggctt cctgggcttc atccagacgt tcgcggattt
     1801 catcgacgcg atccgccagg ctgtcatcag ccaacaggtt gagacgcggc agaatgcggt
     1861 tcagcgccgt aacgccctct ttcgcctgct caaattgaat acgctgctgc tggttatcat
     1921 tttcatgatt actcagcgcc cgctccagtt cgacgcgacg gctgttcagt tgacggattt
     1981 cggcttccgg atcagactca aacgccaccg ccagatgact acctataaag cggctgaacg
     2041 cctgatgcag acgctgggtt ttctgtacat caaaagagag cgtggcgaag cgttcggaaa
     2101 gcacttcacg ctcggcatgg aggctttcaa tacggctttc acgcgcagca cgaccaaaca
     2161 gcggaacttc cggaaaacgt gagtaacgcc actgacgatc ggcgattttc accactaccg
     2221 ctttttccag ctcatcaacg ctgaacacgc tgtcatcgaa tgactgcgga tccccttcga
     2281 tcagatagag atcttccggg caatcggtca agccttccag gtgttcggtt acctgtgaca
     2341 gatctggcac cacgatggcg tggcgtgacg ggccatacag tgccgagaag tacggcgcat
     2401 cttccagact cacgtcgtca taaatttcgg acagcagcac gccgccaaaa cgctccgcca
     2461 gcgcattcaa acgctggtct tcagaaccac ctggctggct taaacgttcg atctcttcat
     2521 cgacggcgtt tttgcgcgcg cctacttcat cgcgttcaac aatggcttcg cgctcacgct
     2581 ccagcaactg ttgcagatat tcagtgacgt cctggctgga ggtgaactct tcgccgcact
     2641 gttcgctcaa ctggttgaga ctgttttgcg ctgccagcca aaccggtgca cgctgcatca
     2701 aactctgaat gcgagactgc agctgttcct gttcctggcg cagtgccatc cgctcttctc
     2761 gggcgttaga gacgctatcg gaaagagagg caatgcgtgc ttccagctcc tgatgcagag
     2821 cttccagttc atcaatatca aaattcttgc cctgacgttt gcagaaatct gccagcagac
     2881 gctcagcttc ttgctgctca cgcagacgct gttccagttc gcttaagcgc atccgcaacg
     2941 gctgaacctg ctctgccagg tgacgctgat cgaccccttc gcgcaatagt tcgcgagcga
     3001 catcccacgc ctcgttacgc gccagcgggc cgttgattgc caccaccagc tgataagcct
     3061 gctcaaactg gctgtgcgcg gtttgcgcca tgctcatttt ctgctcaaga gagagcattt
     3121 tttcggtcgc ttccagctct ttcgcctgga aggtttccag ccattcggca gcgctgtcgg
     3181 cggttaagtc cggcagatgg cacagttctt tggcacgatt aagcgcagca atcgcctggt
     3241 tatactggat cgcgcgcgtt tgctggacgt ccagcgcctg ctggtagtca gcaagctggc
     3301 ttttcagctc gtccacttcc agttcggcag cttccgcacg agcctcgttc tcttcctggc
     3361 gttcgatggc ttctgccacc acttcgtttt gctcttccag acggatctgc agctcatcga
     3421 gatccgcttc gtagcgttcg atcttctcct gctgacgcag tgcggtttgc accaggttca
     3481 ggtgatcgct ggccgcctga taatccgctt ccagatcgcc ttcggcaccg ttgtgctcag
     3541 ccagctctcg cgccatgtcg acgtgtttgt actgctcagc cgccagttgc tgacgcgaag
     3601 tatgtagctc gcgacgaaac tccagggcct tatcgagatg aacacggcgc tcgttggcgt
     3661 gacgcatgta gtccgccgcc acgtagttgg tggcttcgct gatcagatgc ttaaacaggt
     3721 cacggtctga ctgggtgaca cgaatcgctt ccagcgtcat gcggttttca cgcagcgccg
     3781 cttccatgtc ctggaacgct ttccgcaccc cactgttttc cggcaacagg tagtcgcgca
     3841 gagaacgagt aatggcgctg gagatcccgc catacagcga ggcttcgatc agacgataga
     3901 atttactacg gtcagacgct gagcgcagac gacgcgcgat gatgcccaga tcgaacatca
     3961 gcgagtggta atcggtaatg gagttgaact gtttaaactg cacgccttcc atcgcctcga
     4021 gcttgtcttt cagctcgtta agcggcagca cgcgcgcctg gcgttcgttc agagtttcgg
     4081 tcaccagttg tgtcggctgc accgacatcg gcaggccctg aatggcaaat ggtttgatat
     4141 ccactttacg atcgcgtccg gcaacctgtt gcagacgcac accgaccact acacgctggt
     4201 ggcgcgagtt aatggtgtcg agcatcgaat aacagacacc cgctttcagc ttaccgtgca
     4261 gacctttatc gcgcgaaccg ctggtggccc cggcttccgt agtgttacgg aaatgcagca
     4321 gggtcaggtc ggggataagc gccgtaacga acgctgccat ggtggtggat ttacccgccc
     4381 cgttaccgcc agaaagcgtc gtgaccagct cgtcaaggtc aaaagttcgg gcaaaaaagc
     4441 cgttccagtt aatcagcgtc agtgagcgaa atttaccgcg ttcaatcatt attcttcctc
     4501 tccgctatct ggctgactct cttcggtttc atcgttgagt tgcagatgat tttcaatcgg
     4561 cattgcttcg ccatcacgaa tcaggcgacg ctgtgcttca cggggatcgt cgccagcacg
     4621 cacatcggct ccgaagcgga acaccgattc ggtaatgcgg aacttgctgc tgtcgtggcc
     4681 cataaaccac accataccca aacgacgcag acggttgagc gaagaacgta ctttctcctg
     4741 caacttctgc cggtcaacgt ctgaaccggt tgaacggttg ttcaccagtt tcagcagttt
     4801 cgcttcatcg gccagggtga gcagttcgtc gtacagttcc tgctgggtga aaatcccctc
     4861 attcgccagc cgttccgggc tgagatagag ataacagagg attttcccga ccatcatatc
     4921 cagttccgac aagacggaac gagggatcag cgtggtggaa cgtgggcgta gatagaagaa
     4981 cccttccggt gcacgaataa gctcaacgtt ataacgcgcg taaaactctt ccagatattc
     5041 ctgaaaatcc atcaggaatg catgattatc cagttcgtcg aggccaatat gacggcctga
     5101 acgtaaggcg ctatccagcg ccggaaataa cggattcgcc agcgcctgcg ccagcttaac
     5161 cggcatcact tgttcaatat ttgtcgatga catgcgcctg taccttggct ccgtaatcat
     5221 taatcggctg ccatttcgct ggcagtccgg tgaaatctgc ttgcgctacg ccaagacgta
     5281 ccgcctgatc aataacaatg cgcgcaacgt caaagtgacg tgcacgcgga tactgtgaca
     5341 gatattcgcg taccaccaga ccaagatcca acggcacttg tctggttttg tacacggcaa
     5401 gttgttcttc gatgatcgcc gccagctgtt cgcggatctc gttaaactct tcgtactcca
     5461 gatcctcagg aagttcccca gtcacttctt catcgcgcag taccatctct tcgtcacgca
     5521 tatccagcag acgatcggca ttggcataag ttagcgccca cggctcatca aaataggttt
     5581 gtaccgactg acgtaaccgc tgagcaaaga cgcggttttt atccatatcg atcgcagtac
     5641 ggataaattt gtgtacgtgg cggtcgtagc caatccacaa gtctatggat tgctggcccc
     5701 aactgataat acggtcgagt ttgctctgca gatcgaacac cagacgatcg acaaaatgca
     5761 ggtcgtcatg ggtcatcgtc gcatcctgaa tgcgcaacag attagcctgc aatttatcgc
     5821 ctgccgcttc cagcgtatcc tgcaattcac gcagcgttcc ggaagtttct gaaagcaaca
     5881 attcacagct ggaaatcgcc gcccgccagt ctttgttcaa caactgggcg atatcatcct
     5941 tcacctgctg ctgctgttcg tccatcagac gttgcgtcag gtcgatactg tcgaaaattt
     6001 ctgctaccga atatttcagt ggcgcataaa cattacggtg acagtgaaat tcatcaccgc
     6061 cctcttcggc ggcatctgcc gcgcgtttga gctcacccgc cacaatcgac aactgcatag
     6121 aaaggcgcag cgtagaaaac tcgcgctgac ggatgtaata gtcagtaatg ccgatgccga
     6181 gcggcgtcag acggtaaatt gcgttccctt ccgcctgctc gctggtaaag cggttcagca
     6241 gacgttgacg caccatgtcg ttgatcgcgt tgttggcgcg cacgccgatg gtttcgctgg
     6301 tttgctcaaa cgcatcactc acatggcgga atgcatccac cagctcgcct tcactcatct
     6361 caccatccag acgctcgccg ttcagcgtgg caaccgccag cagaaaagag agtcggtcta
     6421 ccggcagcga gatggagaag tcattttttc tggcccaggc aaccagttcg gggactgtct
     6481 gggaaaattc actcatactt tatccttgct ctgcggtttg cgcgcggtga catgaatata
     6541 acgccccagg gtaatatacg gttcctgacg gcaataacgc gtttctaatt caagtaatgc
     6601 ttcatagcag tcgcgctgct ggtgcttctc gcgcagataa tcatgaaaca cgcgaacgcc
     6661 tgtcttaccc ataatttgcc aaccagcttc ttccagccac agataaacct gcgccgggtc
     6721 gcgtggataa tctggcgaaa gcgtccgttt tttctttttc ggcattcccg cctgcacgta
     6781 atcaaaattc ccggcgacca tgttatgcat cagcaagccg tgcgcattgt agaacattaa
     6841 cgacaacaca ccgcctggac gcaacactga ccacagggtc tgtaatacgc tgcggggatc
     6901 ggccacccac tcgagcaccg catggaacaa tatcagatca acgggcgttt ccaaatgtga
     6961 agcaacatcc tgagcggcgc aatgtataaa ttgcatgttg tcgctcacac cttttgcttc
     7021 tgccgcctgt tttgcgcggt cgatcatctg cgcagaaaga tcgcataaaa tgacctgatg
     7081 cccgcgctca gccattttga ttgcggtctg cccttctcca ccgccagcat ccagcacacg
     7141 cagtttttgc gggcccattt ccgccagcac gcgatcgaga tcctgccaca gaatagcctg
     7201 tcgaagctgc cctttggtgg tgccgtaaat gttacgggaa aacttttccg caatatcatc
     7261 aaaattgcga tcctgcattg ctttctccac cggttggcta agacgttttc tgataaagac
     7321 gctattttgt cacagccacg gcgagaatga acccgtctgg tgtaatatcg ctggcgatcc
     7381 cttgctatat ggttaaaaaa ggaaccagaa aggatgcttt ttacactgaa aaaagtgatt
     7441 ggcaatatgt tgctgccact tccgctaatg ttgttgatta ttggcgctgg cctggcgctg
     7501 ctgtggttta gccgttttca aaaaaccggg aagattttta ttagcattgg gtggctggcg
     7561 ctcttgttat tgagtttgca accggtagcc gaccgcctgc tgcgtcccat cgaaagcacc
     7621 tatccgacat ggaataattc gcaaaaagtg gactatatcg tggtgctcgg aggtggttat
     7681 acctggaacc cgcagtgggc accaagctcg aatttaatca ataacagtct gccccgcctg
     7741 aatgaaggta ttcgcttatg gcgcgaaaat ccgggatcaa aactgatctt cacgggaggc
     7801 gtagcaaaaa ccaatacggt gagtacagcc gaagtaggtg cccgagttgc gcaatcgctg
     7861 ggcgtgccgc gcgagcaaat tatcactctg gatttgccaa aagataccga agaagaagct
     7921 gcggcagtga aacaggcgat tggtgacgcc cctttcctgc tggtgacatc cgcgtctcat
     7981 ttgccgcggg cgatgatctt ttttcagcag gaagggttaa atccgctgcc agccccggcg
     8041 aatcagttgg ccatcgactc gccgcttaac ccgtgggaac gggcaatccc ttctccggtc
     8101 tggttgatgc atagcgatcg tgttggctat gaaacgcttg gccgtatctg gcagtggcta
     8161 aaaagttcgt caggcgagcc acggcaagag tgattttgat gccagatcga agttgcgccg
     8221 actaaatcgt ccggtattaa ccagttgcgc aacttcatcc cataacacat acaaccaacg
     8281 ccgccagatg aacgactccg ccactggcgc gcgttgcagg taactccaga gcaaatcttc
     8341 cgccagagaa gtatccatta gtcggaacag ttcgtattcc cgcggtgccc aaagcattag
     8401 tcccggcccg accatcgcca gtaactgatc gctgcgcgaa tctttcagca tgctgcgtaa
     8461 acagaagtta ccgtgaatca gcacacaatt gtcgttaaag ccttcaaata atgccgggag
     8521 acattcgcga gtacgaaaca ggatccgctt atcctgcatc gttagaccgg tgttattgaa
     8581 ctgattgagc gtggtccata gcacttcaac atgttgccgg taccatgagg gccagaaatt
     8641 ttcctgagta ttgtcgaccg cgccgacgca accgcgactg tcctgacggt gccaggccag
     8701 taaggcttca acgatttgat ctttgagttg ttcccagcgt tctggtgttc gggctggtgc
     8761 ctccaccgaa acaccacgca tccgctccag tagcaggaca tcggggccgg ggtgttcttc
     8821 atgcgtcatc acaccataaa tagtcggcat acggacagtc ccacttcttg ccagcatggt
     8881 ggttttccat gccagttgtc gggcttttcc gggcgtacta aagcttcttg ccattaacgg
     8941 cattgggttt ccctggctgt catacaaagc ccacaacgcc gtatccgctt tttcattgac
     9001 gcactcaata cgactgagtt tttcgcccag taaatggctt aattcggcac gcagctgttc
     9061 cattcagatt accctcataa aagctactgc tttcataatg agcgccaata taaaagatgt
     9121 cacctgaatg agatcaattg ttgaaaagaa aaattgagga aaatcgtaac ggtacgacac
     9181 tcctcccaaa attggctgga gtgtcgtgaa gtgaaattag cgcatttcag cgcgaacgcg
     9241 ctcgagatct tcaggggtat ccacacctgt gccaggaact tcctgggcga cagcaacatg
     9301 gattttttcg ccgtaccaca gaacacgaag ctgctctaac atttcgatgt gttctaacgg
     9361 acttggctgc cagttgacgt aacgacggat aaagcctgca cggtagccat aaataccaag
     9421 atgacgcagg aagttatcgc caacggtttc aaggcctttt gcaaaacgat cacgatccca
     9481 aggaatggtg gcgcgagaga agtacagtgc atacccttca gcgtcgagga ccactttcac
     9541 cgcattcggg ttaaacgctt cctccgcatt gtggattggc accgccagag tcgccatacc
     9601 cacctgacgc tgagcgaggt tatcagcaac ctgacgaatg attgtcgcag ggatcatcgg
     9661 ttcatcaccc tgcacattaa cgatcacagt gtcgtcgctg aatgcgcatt tttcgacaac
     9721 ttccgccaat cgttctgtac ctgactgatg atcggcgcgc gtcatacata cttcgccgcc
     9781 agcggcttca acagcgcggg caacatcctc atgatcggtt gccacgatga tgcgctcggc
     9841 acctgattcg cgcgcgcgtt caagaacatg aacaatcatg ggtttgccgt taatatcaac
     9901 caatggttta ccgggcagac gcgtggacgc gtaacgcgcg ggaataatga ccacaaaact
     9961 catgatttac tctcatcagc agtcagcacg cgggcttccg tttccagcaa cactggaatg
    10021 ccatcgcgca gcgggaaagc caggttgtcc agtttgcaaa taagctcttg tttttcctgg
    10081 ttataccaaa gttttccgtt gcaaaccggg caggcaatga tttcaagcag acgatgatcc
    10141 atagttcctc cagatggatc gggttatgaa tgcataaatc ttatcataat catttatgcg
    10201 acggggtcta tttcccagcc ggcgcggcaa tcagtaaaga ggccttgcgg gcagcgtcct
    10261 aatgtcaccc gcgttgcctg ctgccagtta gcgaaatcag taatcgcctg acgtaaccct
    10321 ttttgcagcg ttgtcgttgg tttaatgcct tcttgtaacc acagagagat aacttcaagg
    10381 atgcctgtct ggcgatgcat tttggcatcc attcgcccaa ctaattgccc acgatgtaat
    10441 aacggcagaa caaaatagcc atactggcgt ttcggcgctg gggtatagca ctccagccgg
    10501 tagctaaaat caaaaagctg ctctgcgcgt ttgcgatccc agacaacagg atcaaaaggc
    10561 gaaagtaccg cgctgtgcgt tgcagtgagc tttccggcca gcgcacgctc gagtagcggc
    10621 agcaaatcag catgcagcca aagattgccc aatttttcaa catgcacagc aatgatttgc
    10681 tgctgttcag ccctcgcttc acgccacgcc gccagtgccg gacgttttag ccggtagtaa
    10741 tctgccagcc actgttcgcg aaatataccc aggctacgcg cactgttatc cagcatgatg
    10801 atttctgctt ctgcttgcga aacgagatcg cgctcatcat tccactcagg catgacacgg
    10861 tgagttaaat cataaacgcg ctggaagttg cgccgttcaa tcaccatcac ctttccggca
    10921 gtaaataaac cttccagatg tcgtttatgc ggcttccact cccaccagcc gctcgcccct
    10981 ttacgaggat gctcaaaatc ggctgaacgt actggcccct tatcatgaat atgctgaatt
    11041 aactgtgcaa tttccgccgc atgttcctgc atccaggcgt ctttatattt ccagcccatt
    11101 ttttcaggtg ccagcatgcg gtggcgaata agacgaaaat cgctacgcgg cataaagcag
    11161 gcttcatgcg cccagtattc cattaattcg ccacgcgcca gagactcatc cagccactgg
    11221 gcaggataat ttcccagacg actgaaaagc accagatatg gactacgggc aacaatattg
    11281 atggtatcga tttgcagcaa ggacatgcgg gagatcgttg ccggaatatc ccccaacgac
    11341 gctcgacggc ggggtttgtt taacaggcct tgcgcggcga ggtgaagatt acgcgcatca
    11401 gcaagagaga ggtgcggcag cgacattcat gactccatca atcgaacgct gccgcggcgt
    11461 aactagttgc cagaagccag cgaggttagt tgcgcaagca gtttcgctgg ttcatcacct
    11521 gaaagctgtg cgtcaacagg caaataccac caatttcctt cggcaaaggc ccggcatttc
    11581 accgcatctt tttcagtcat taccagcgtt tgcccggcgt ttaccaacgc gctgacatcc
    11641 gcatggttca aagactgatg atcggccagc ggtacacatt tttccggttg tacgccgcac
    11701 atcttcagcg tggcaaaaaa gcgcggcgga tgtccaatcc ccgccatcgc cactacatgt
    11761 tcaagctgag caacgtcaca acgcgtaccg gtacgtaaat tcaccgcctg acccggcagc
    11821 agatgcatgg ggatttcacc gctgcggggg acaccgccgt tgacgattac cgcatcaacc
    11881 gactttaagc gccccgctcg ctcacgcatt ggccccgccg gcaaccacca gccattgcca
    11941 aagcgacgca caccatcaat aacgacaatt tccacatcac gcgccagacg ataatgctgt
    12001 aaaccatcgt cggttacgat gatctgcaca tcagggtgtt gcgccagaat ggcttttacc
    12061 gcgtcagaac gcacgggaga aaccgcaaca ggcgcgccag tgcgttgata aatcaacaca
    12121 ggttcatcac ccgcctgtgc tgtggtggta tctgccgaca ataacagcgg ataagattca
    12181 gccttaccac catatccccg cgatacaacc cccacgcgaa taccgcgctg ttgcagctgt
    12241 tccaccagcc agacaacgac cggggttttt ccgttgccgc ctgcggtgag attaccaacc
    12301 acgacaaccg gtacgggggc acgccaggcg cgcttcagtt ttagtttata gcaaagacgg
    12361 atcgcgccac tcaccaggcc atacaaccag gagagtggca gcaatagccg ccataaaggg
    12421 gattcaccag accagatttt ttcgatcatt ggccaaactg cattttgtga agttgcgcgt
    12481 aaacgccacg gtgctcaagc aaatcgttat gcgtaccgcg ttccacaatg acaccatcct
    12541 cgacaaccac gatttcgtct gccttttcaa tggtagacaa gcggtgggca atcaccagag
    12601 aggtacggtt tttctgcaac tcatccagtg ccgcctgaat cgcacgttcg gattcggtat
    12661 ccaacgccga ggtagcttcg tccagaatca gtatcgggct atcacgcaac aaggctcgag
    12721 cgatagcaat acgctgacgc tgaccgccag agagcagcac gccgttttca ccaatcactg
    12781 tatcgagacc gttatccatc ttattgatga agtccatggc gtaggccata cgcgccgctt
    12841 cttcaatttg ctcacggctg tactgttcag tccgtgcgta agcaatgttg ttagcaaccg
    12901 tatcgttaaa cagatggaca ttctgcgaca ccagagcaac ctggttacgt aacgacgcca
    12961 gggtatactc gcgcagatcg tgaccatcca tcaggatttc gccttcatca atatcgtaaa
    13021 aacgcgtgat caggctggcg atggttgatt tacctgaacc agagcgtcca accagagcaa
    13081 ccgtcttccc tgccggaatt ttcaggttga tgttacgcaa tgcgggcaca tcacgtcccg
    13141 gataagtaaa ggtgacattg cggaattcca cgtcgccagt cgcacgctcg atcacgcgct
    13201 taccttcgtc tttctcctgc tcactgtcca gaatggtaaa cagcgtctga caagccgcca
    13261 taccgcgctg gaactgggcg ttaacgttgg tcagcgattt cagcggacgc ataagtgcaa
    13321 tcattgaaga gaaaacaacg gtaatcgtac cggcagtcag gctatccatg acacttggga
    13381 agcttgccgc atacagaaca aacgccagcg ccaaagaggc gatcagctga atgatcggat
    13441 cagagatgga agaggctgaa accattttca tcccctgaag acgcattcgg ttgctgactt
    13501 tatcgaagcg tttcgtttcc acttcctgac caccgaaaat caatacttct ttatggcctt
    13561 tcagcatttg ttcggcgctg gtggtcacct gccccatggt gttctgcatg tttttactga
    13621 tgttgcgaaa acgcttcgat acaacgcgaa tcgcaatcga aacaatcggt gccagcacaa
    13681 tcaaaatgat cgacagttgc caactgtaat agaacatcat gatgaacagg ccgatgatcg
    13741 acgcaccttc acgtacaaca gtaatcagtg cgccggaaga agaagaagcg acctgttcgg
    13801 aatcgtaggt gatacgtgac aacagcgtac ccgttgactg tttgtcaaag aatgaaaccg
    13861 gcattcccat catgtgacca aacaggcggc gacgcatagt cattaccacc tttcctgata
    13921 cccaggagat acagtagctg gagatatagc tggtgatacc acgtaaaatc atcagcccga
    13981 tcaccaccag cggcatccac atcagcacgg agcgatctgt tttaccaaag ccatcatcaa
    14041 gaagtggctt aaggagcgat aacatgaagg tatcgctggc tgcgttgagg attaacgcta
    14101 cgcccgccac gatcagaccc gctttaaaag gcgcaatggt tggccacagt cggcggaatg
    14161 tctgccacgt agagagatct ttgtcgttat gcattcaaaa aaccagcatt tgttgaaata
    14221 gccgcatatt ctacccgtta tccactggca cgccaaacca ctgatgatac caacgaggta
    14281 aaatttgctc tctgaggctg ctaatccgcc agccttgcgc tgaaaaattg accgttattt
    14341 gaccctgatg tggagtatca agccacttat aaccctgttg ttgatagcga tgtttaactt
    14401 tgcttgaggg cagtcgccat gcgttatagc gcgatgccga tgcgagtgcc acttttccat
    14461 tcactcgctg aattaatggc aacgatgatg aggtattact gccatggtga ggtacctgaa
    14521 gtaatgttgc ctgcatttgc tgccagtaac ggcttagcat cttttgttca gcgggggctt
    14581 caatatcacc ggttagaaga atgctattcg tcccgtcatc aatcttaacc acacaggaat
    14641 ggttatttcc tttatcgttg ctaccttgta aaggccagtg cgcgctgaaa cgcaatcctt
    14701 gccattgcca cgcttcgcca cgcacacagg gctgatgatg ttcccagttt aactgacttc
    14761 ggatccataa catcggccat gtgtgcaata ttgagtccag ccctccccgg tgatccagat
    14821 gttcatgact caaaataacg ccttccggtt caagattatg ccagtggagc caggggataa
    14881 tcagttgttg cccactatcc ccttcgggcc aggccagtcc cgtgtcatag agaatcgctt
    14941 tgccgtttcg cgcaatgacc attgccagcc cttgcccgac atcaagcatg tacacttgcc
    15001 actcgtcagg tcgaggtttt tgccacagcg gcaagcacat cagcaagcct acagccacac
    15061 acattgctgg caacgttcgc caggcgttta atcgccatac caccagtaag aaccatgggg
    15121 aaaatgttag ccattgccaa cgttcagcaa tgttgatcca cccttccggc aaactcttta
    15181 acccccagaa aagtaaagcc aaagaccggt cggcaagaaa ccataaccct tgttccagaa
    15241 taaacggccc gcttaaatgc gcaaccatcg cggcgaggat caacggaacc gtgataaatg
    15301 tcaccagggg aattgcaaat agatttgcaa taaacgaggt cagactaatg ccatgaaata
    15361 tgacgatttg cacgggcata agcaggagtg tgattcccag ttgtaaatga atgagggaaa
    15421 caactgcacg caataccggc ggcagttgcc actccggaca gggaaaccac tggtaccaaa
    15481 aaatcagtgc cgcgaccgca gcggcagaga gccataaact ttgcgagaga atggcaacag
    15541 gatccatcag caaaattgcc gccagacaac atatccatac atcccagcca ctccattgtc
    15601 gcccacttaa cttaagcatt ccccacatgg caagcgccac cacggtacgc aatgcaggag
    15661 gttgcatccc agtcagccag gcataaaaag cagcacagca gattccgcca attaatggca
    15721 tttgccagtg gatccagcgc ccaggcagaa aaacttgccc accgcgaatg agtccggcag
    15781 ccagcaacgc cgcaaaagcg atatgcaatc ccgatatcgc cattaaatgc gccgttccag
    15841 tatcgcgcat gatatttttg atttctttgg gaacggataa ccgttccccc atacctaaac
    15901 caagaataac cgcgttccac atatagggtt gcagcgttgt ttgtagtgac gccagatact
    15961 gtgcacgcag gctacaattc ggttcaatga cacttgcctg cagaaagcgg ccggtgagcg
    16021 gctgatgctg ggcaatggca taacgctggc tatcaaagcc gccatcatta agttgaccat
    16081 gaactgcacg aactttgagt ttcattgacc acagttgtcc agcacaaacc gcttgcggaa
    16141 gatattcgcc atacagcacg aggcctggcg cagggaatat acgtcgacct tgtagatgag
    16201 taatttgacc gtaatgagtg gtcataccgt cagtagcagt gatctcaaca attgcatcct
    16261 gcgtcgcgcc agttaaggtt tctcctgccc acaaaatttg ctttgctgct aatatgcccc
    16321 aaacgaaaaa cagcaacgtc agcgcgatat aacggacagt tttaaaaggg ataaatacca
    16381 gtacgcaggc gaagacggtc agagacgcaa gggttaatgt cccaggcaat tggggcaaaa
    16441 tcagcaacgg aaaaattccg caaattatgc atacgccgac tgtcgttatt ttcatgcacc
    16501 cctccctgtt tatggaaagt gtgcaacttt gttgagcggt tgtctggtgg gagttttgcg
    16561 ttccccgagg cacgttcaga agaatttaac gagagggtga aaactgttca aaactctgcg
    16621 aggctgcttc cagttccaga ttagagaaat gaaaaaagca cccgacaggt gcttttctcg
    16681 cgttcaagtt tgagtaaaaa cttaaccgta aatattggcg cgatcgcgca gttctttacc
    16741 tggtttaaag tgaggaacgt attttccttc cagttctact ttatcgccag tcttcggatt
    16801 acgtccggta cgtggtgcgc ggtagtgcaa agagaaactg ccgaaaccgc ggatttcaat
    16861 acgctcgccc tgcgcaagag tcgaggccat atgctccagc atctctttta ctgcatcttc
    16921 aaccgtcttg gcgggaatgt gcgattgctg ggtggcaagt ctttctatca attctgactt
    16981 ggtcatgatt cctccggttc cttaaaggca aatttagcgg ctgctgttgc tgcattgatt
    17041 aagggcggcc gtagccgccc ttagtgcttg attacaggac gaaacctgca atctgtcaag
    17101 taaactcaac aaacttcgga ataaaaatcc cgaagagtca gagaattact cgcctttagc
    17161 tgctttgaaa gcttcagcca ttgcgttgtt ggagaagttt gcatcttcct gtttgttaac
    17221 agttgcgatt gcatctttct cgtcagcttc gtctttcgca cgaacagaca ggctgattgc
    17281 gcggttttta cgatcaacgc cgatgaattt agcttcaact tcgtcgccaa cgctcagaac
    17341 cagggtagcg tcttcaacgc ggtcacggga tgcttcagaa gcacgcaggt aaccttcaac
    17401 gccgtcagcc agttctacgg ttgcgccttt agcgtcaact gcagttactt taccggttac
    17461 gatagcgcct ttcttgttca gagcaaccca gttgttgaac ggatcttctg cgagctgttt
    17521 aacgcccagg gagatacgtt cacgttctgc gtcaacctgc agaacaactg cagcgatttc
    17581 gtcgcctttt ttgtattcac gaactgcttc ttcgcctgca acgttccagg agatgtcaga
    17641 caggtgaacc aggccgtcga tgccgccgtc caggccgatg aagataccga agtcagtgat
    17701 agacttgatt ttaccttcaa cacggtcgcc cttgttgtgg gtttccgcga actgctgcca
    17761 cgggttagct ttgcactgtt tcagacccag ggagatacga cgacgttctt cgtcgatatc
    17821 cagaaccata acttccacta catcgccaac gttaacaact ttggacgggt ggatgttttt
    17881 gttggtccaa tccatttcgg aaacgtgtac caggccttca acgccttctt cgatttcaac
    17941 gaagcagccg tagtcggtca ggttggtcac gcgaccagtc agtttggtac cttccggata
    18001 acgtttagcg atagctaccc acggatcttc gcccagctgt ttcaggccca gggatacacg
    18061 ggtacgttcg cggtcgaact tcagcacttt aacagtgatt tcgtcgccca cgttgacgat
    18121 ttcgctcgga tgcttaacgc gtttccaggc catgtcagtg atgtgcagca ggccgtcaac
    18181 gccgcccaga tcaacgaatg caccgtagtc agtgaggttc ttaacgatac ctttaacttc
    18241 catgccttcc tgcaggtttt ccagcagctg atcgcgctct gcgctgtttt cggattcgat
    18301 aacggcacga cgagaaacaa caacgttgtt gcgcttctga tccagcttga ttactttgaa
    18361 ttcaagctct ttgccttcca ggtgcagagt gtcacgcacc ggacgaacgt ctaccagaga
    18421 acctggcagg aacgcacgaa taccgttcag ctcaacagtg aagccgccct taactttgcc
    18481 gttgataaca ccggtaacag tttcagcatc ttcgtaagct ttttccagcg tgatccaggc
    18541 ttcgtgacgt ttagctttct cacgggacag cagagtttca ccgaagccgt cttctactgc
    18601 gtccagagca acgtcaactt cgtcacctac ctggatttcc agctcgccct gggcgttttt
    18661 gaactgctca gccgggatgg cggactcaga tttcagacca gcgtcaacca gtactacgtc
    18721 tttgtcgata gcaacaacaa cgccacgaac gatagaaccc gggcgggttt cgatttcttt
    18781 taaggactct tcaaagagtt gagcaaaaga ttcagtcatg tttaatcttc aggtttatat
    18841 ttaacgtcca cctggctcca tgccggatgg ggttgtttaa catacccgct gtcattccat
    18901 tgcaacgggg gtactgcaaa ttcggtcgct tatgcgagag ccaatttctg gcgcgcgtat
    18961 tgtagcgctt tttcaatcac ttgctcaatg cttaaggtgg tggaatccaa cactaaagca
    19021 tcggctgccg gaaccagtgg cgctaccgct cggttacgat cgcggtcgtc gcgttctttg
    19081 atctcagcca aaaggcgctc aaagttaaca ctaaagccct tctcctgcaa ctgtagcatg
    19141 cggcgatgcg cacgttcttc cgaggaggcg tcaaggaaaa ttttcactgg cgcatcaggg
    19201 aataccaccg ttcccatgtc acggccatcg gcaatcaggc ctggtaattc gcgaaacgcg
    19261 cgttggcgac gcaataatgc ttcacgaacg cgtgggaatg ccgcgacttg tgaagctgca
    19321 ttcgccactt cctgagtacg aatttcgcca ctgacatctt ccccttcgag gatcacttcc
    19381 agattgccat tggtcgacac aaaacgtaca tccagatggg atgccagcgg caccagcgca
    19441 tcttccgatg caacatcaac atggtgatgt aatgccgcca gtgccagtac gcgataaatt
    19501 gcacccgagt ccagcagatg ccattgcaac gcttccgcca tagccttaca caaggtgcct
    19561 ttccctgcac cgcttgggcc atcaatggta ataaccgggg caattgccgt catctttatc
    19621 tccttaaaac aggcgtaccg ttaacataac cgcgcgcatt atacgcgccg acgccacaaa
    19681 tcgttaacgt tgtgtgcaaa aaccagtttt gtctgtattc agatggggat ttattgcgca
    19741 attatagcgg gctggcagaa gaccagcccg aaagatgaca atgatttact taccagaggc
    19801 gatacgatca cggatgtgtt gcgcacgctc tgttgatggt gggtgagaat caaacatgga
    19861 ctgggtacga ccgccatcca ggctggccaa tttttcaaag ctgccaacca gcccctgggt
    19921 gctgatgcca cgtttcttca acagatcaaa ggagaaatca tctgcttcgg actctttatc
    19981 gcgggagtat ttagcattga tcgcgccttc tgcgatatca cctaattgtg agcgagaaag
    20041 ctgggaagcc acaccgctgg tagcggaaat cgcatcacgt gcggcaacga tcgcataaga
    20101 agctttcatt tcagccagcg agtgacccaa cgcgacgtgg cccagttcat gacccagaac
    20161 gccttcaatt tcgttgtcgt tcatcatgtc catcaggcca ctgtagacac gaacacaacc
    20221 gttcgccatc gcccatgcgt tgacgtcgct ggtcatataa accttatagt tgaccggcgt
    20281 gccattaata ttgttaccca atgctttggc gatttttgcc agacgtttcc cgtacttgct
    20341 ggatttgctt gcgacttgat tgccgctgtc catttcggca cagccctgat tggcaatcgc
    20401 ttttgcatca gcatcgctta acgttgctgc tttataggca tttaaacctg agctgatagc
    20461 catattggta tcaataccgt gggtattttg acaccctgtc agtaatgctg cagaggccgc
    20521 aatcgccagc agtaatttag tattcttcat gtgaatcatc cgttgattaa tcatatataa
    20581 atattatgta tcaataactt acattgacaa gcgacgctac cattaatcaa aatgactcac
    20641 aaggtccgaa aaaaaacgcc ggcaatgtgc cgacgttttg ataaagaaag atttggctat
    20701 ttattgcacg ttgttcattc aggctgcctg gctaatccgc gccagctgct cgaaataatt
    20761 cggaaatgtt ttggccgtgc atttgggatc aagaatcgtc actggtgtat ctgacaacgc
    20821 caccagcgag aaacacatcg ctatccggtg atcattgtat gtcgcgatct cggcaaagtt
    20881 cagcttttcc ggtggagtga tacgaatgta atcgtgcccc tcttctactt ccgcaccaac
    20941 tttacgcagt tctgttgcca tcgcaaacag gcgatcggtc tctttaacac gccagttata
    21001 gatattgcgc agcgtggtgg tgccttttgc aaataacgcc gccgtggcaa tggtcatcgc
    21061 cgcatcggga atatggttca tatccatatc aatagcgttc agttcacccc gcgtgcagga
    21121 aatataatca tcgccccagc aaatggtcgc gcccattttt tccagcacat cagcaaagcg
    21181 aatatcaccc tgcatactgt tgcgtccaat gccggtcact tttacagtgc cgcctttgat
    21241 tgctgctgct gccaggaagt aagaagccga agatgcatcg ccttcgacca aataagtacc
    21301 cggagactga taagactgcc cgccttttac gacaaattgt tgatagtgct gattttcaat
    21361 ttcaacacca aacgtcttca tcatattgag tgtgatgtcg atataaggtt tagaaaccag
    21421 atcgccttta atacgaatca ccgtatcttc cggtgcaaga ggcgcagtca ttaatagtgc
    21481 ggtgaggaac tggctggaaa cggagccatc aacgtcaacg ttgccgccag taaagccgcc
    21541 ctgtaaacgc aatggcggat aattgtcttg ttccaggtaa gtgatcttcg ccccgcccag
    21601 gcgcagcgca tccaccagat gaccaatcgg gcgctctttc atacgcggct caccagtcag
    21661 cacaatatca ttgctaccca gacaaagagc tgccgccagc gggcgcattg ccgttccggc
    21721 gtttccgagg aacaactcca gggcaccttc tgcgtgtaat ggaccgccgt taccgataat
    21781 ttcgcaacgc gtacgatcgg ctgaaagcgt atagcttacc cctaacgctg ttaatgcatt
    21841 cagcatatgg cgcacgtcat cgctatccag cagattggtt aatactgttt tgccgtgtgc
    21901 taatgccgcc agcaataaag cgcggttaga aacgctcttg gaaccaggca gattaatagt
    21961 gccatcgaca cgagcgatgg gttgtaacgt cagggattcc atgaactcaa ctctctacaa
    22021 cagaaataaa aaccccacag gctggctgtg gggattaagc aaaatttcgg cattagccgt
    22081 gacggcgttc gaactcaacc atgaagtctg tcagggcttt aacgccttcc agcggcatgg
    22141 cgttataaat agacgcgcgc attccgccga ccacacggtg acctttcagc gcatgaaggc
    22201 cagcagcaaa agactcttca aggaacaatt tgtcaagcgc actgtccgcc aactggaacg
    22261 gcacgttcat ccgcgaacgg ttagctttcg ccacgtcatt gcggtagaaa ttgctgttat
    22321 caatcacccc atacagcagt tctgcttttt gctgattgat tttatccatt gcagctacac
    22381 cgccgttcgc tttcagccat ttaaagacca gaccagacag ataccaggca aatgtcggcg
    22441 gcgtgttaaa catggagtcg ttatcgttga gaatggaata atccagaatc gacggacacg
    22501 cgatattcgc tttgcccagc aaatcttcac gaacgatgac gattgtcagg ccagccgggc
    22561 cgatattttt ctgcgcgcca gcgtaaatca cgccataacg gctgacgtca atcggacggg
    22621 aaagaatggt tgaagagaag tcggcggcga ccaccacatc tttgccgaag tctggcgttt
    22681 cgtcgatagc gataccgtcg atggtttcat tcgggcagta atgcatataa gcagcattat
    22741 cagagagttg ccattcgcgc attggcttaa ccgcgcgcag accatcaaca gtcactttgg
    22801 cgtcaaagac attaggcgtg cagtattttt tcgcttcttt aatggcactt gccgcccagt
    22861 aaccggcatc aacataatct gcggtggttt tatcaccgag aatattcagc ggaacagcag
    22921 caaactgacc gcgaccgccg ccatggcaga acaatacctt gtagttggag gggacattaa
    22981 gaagatcgcg aaaatccttc tcggcttcct ctgcaacctg aatgaactct ttgccacggt
    23041 gactcacttc catcaccgac gtaccaagac cgttccagtc gcgcagttcc tgttgagcct
    23101 gtttaagcac ctctgccggt agcattgccg gaccagaact aaaattgaag atttgagcca
    23161 tttcccctca ccacgttgcg ttgctattaa cccgcggtca atcgattgcg accacaggta
    23221 atacattatt cctgtggcta tcggttttat cattcagtga cacacatcgc aatgagtaaa
    23281 accgttgagg gcaaaaatgt ggcttgcatc acacaaaaga atctctcgga ttattagcaa
    23341 aaagccgatg tttttactgt cacgtcaccc gttttgcccg acttgccttc gtccattctg
    23401 gatttgcaca gcgcggacat aattgttttt cccccgcgtt catatgaatg atttcactgc
    23461 aacgaatgca ggcatagtcc accgactcgg gtaccacttt gtaacgctga gtacagtcac
    23521 tgggtaaaat aagtaagccc ggtttcacct tgtcatcttc aaagaaatag acactgattt
    23581 gcccgttggt ttcgagaatc gccagacgta cttgccccaa ctgctctaca ccgcgcaatc
    23641 gtagctccat aaagaactca aattccgtca tgttggagtt attgagtttc gaccaggcca
    23701 gctcgccatc ttcaataatg acaaccggct tgccttccag aagatcttcc agtttttcac
    23761 tgtgcgccat caaccacatt accaggcggt ataacaacgc cagaataata aagacgataa
    23821 gtaccgggac catcggcaca tcatcataaa acgccacatc tcccgccgct gatcccagcg
    23881 tcagaatgat taacacttca aaaagcgaca tctgccgcac accgcgtctt ccggtcattt
    23941 tgaggaacaa aaaaaccagt acaaaggtat aaagactacg tagtgccact tcgccaagga
    24001 aatcaaaagg cactttatca aatgccatac ggtggagatc gaacgccttc atttatatgc
    24061 tcttatcgtg gtttatccgc taaaagcata gtcaacagat catcggcatg gcggcggtga
    24121 tagcgccttt gagataaaac ccgtattatt gcgcgctttc cgtacgacta aagtgatttt
    24181 catgacgcaa acatttatcc ccggcaaaga tgccgcactg gaagattcca tcgctcgctt
    24241 ccagcaaaaa ctttcagacc tcggctttca gattgaagag gcctcctggc tgaatccggt
    24301 gcctaacgtc tggtctgtac atattcgcga caaagagtgc gcactgtgtt ttaccaacgg
    24361 taaaggcgca accaaaaaag cggcgctggc ttctgcactc ggtgaatatt tcgagcgtct
    24421 ctcaaccaac tacttttttg ctgatttctg gctgggcgaa accatcgcca acggtccgtt
    24481 cgtgcattat cccaacgaaa aatggttccc actgagcgaa aatgacgatg tgccagaagg
    24541 gctgctcgat gaccgtctgc gcgcatttta cgatccggag aatgaactgg ccggcagtat
    24601 gctgattgac ctgcaatccg gtaacgaaga tcgtggtatt tgcggtctgc cgtttacgcg
    24661 tcagtctgat aatcagaccg tttatattcc gatgaatatc attggtaacc tgtatgtttc
    24721 taacggtatg tccgctggca atacccgtaa cgaagcacgc gttcaggggt tgtccgaagt
    24781 tttcgaacgc tacgtgaaaa accgcattat tgctgaaagc atcagcttgc cggaaatccc
    24841 ggcagacgtg ctggcgcgtt acccagcggt ggttgaagcc atcgaaacgc tggaagcaga
    24901 aggtttccca atctttgctt atgatggttc gcttggcggc cagtatccgg tgatttgcgt
    24961 ggtactgttc aatcctgcta acggtacctg ctttgcctct ttcggtgcgc atcctgattt
    25021 tggcgtagca ctggaacgta ccgtgaccga gctgctgcaa ggtcgtggcc tgaaagattt
    25081 ggatgtgttt actccgccaa ccttcgatga tgaagaagtt gctgaacata ccaacctcga
    25141 aacgcacttt atcgattcca gcggtttaat ctcctgggac ctgttcaagc aggatgccga
    25201 ttatccgttt gtggactgga atttctccgg cactacggaa gaagagtttg ctacgctgat
    25261 ggctatcttc aacaaagaag ataaagaagt ttatattgcc gattacgagc atctgggcgt
    25321 ttatgcttgc cgaattatcg tgcctggcat gtccgatatt tatccggctg aagatctgtg
    25381 gctggcgaat aacagtatgg gcagccattt acgtgaaacg attctttcgt taccaggcag
    25441 cgagtgggaa aaagaagatt acctgaacct catcgagcaa ctggatgaag aaggttttga
    25501 tgactttacc cgcgtgcgtg agctgttggg tctggcgacc gggtcggata acggttggta
    25561 caccctgcgt atcggtgaat taaaagccat gctggcgctg gctggtggcg atctggaaca
    25621 ggctctggtc tggaccgaat ggacgatgga gtttaactca tcggtattca gcccggaacg
    25681 cgccaactat tatcgctgcc tgcaaacgtt gttattactg gcgcaggaag aagatcgcca
    25741 gccgctgcaa tatctgaatg cgtttgttcg catgtacggc gcagatgccg tggaagccgc
    25801 cagtgcggca atgagcggcg aagcggcgtt ttatggtttg caaccagtag atagcgatct
    25861 gcacgcgttt gctgcacatc agtcgttgtt gaaggcctac gaaaagctgc agcgcgccaa
    25921 agcagcattc tgggcaaaat aaaatcaaat agcctacgca atgtaggctt aatgattcgt
    25981 ctgagt
//
LOCUS       DAZGQL010000076        25790 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.076, whole genome shotgun sequence.
ACCESSION   DAZGQL010000076 DAZGQL010000000
VERSION     DAZGQL010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25790
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.076"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            326..667
                     /gene="raiA"
                     /locus_tag="ACHKIR_003500"
     CDS             326..667
                     /gene="raiA"
                     /locus_tag="ACHKIR_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFO9991186.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    770..895
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            771..818
                     /gene="pheL"
                     /locus_tag="ACHKIR_003501"
     CDS             771..818
                     /gene="pheL"
                     /locus_tag="ACHKIR_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HFO9991187.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            917..2077
                     /gene="pheA"
                     /locus_tag="ACHKIR_003502"
     CDS             917..2077
                     /gene="pheA"
                     /locus_tag="ACHKIR_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HFO9991188.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPQWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGDITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2120..3241)
                     /gene="tyrA"
                     /locus_tag="ACHKIR_003503"
     CDS             complement(2120..3241)
                     /gene="tyrA"
                     /locus_tag="ACHKIR_003503"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HFO9991189.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAAEIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRH"
     gene            complement(3252..4322)
                     /gene="aroF"
                     /locus_tag="ACHKIR_003504"
     CDS             complement(3252..4322)
                     /gene="aroF"
                     /locus_tag="ACHKIR_003504"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HFO9991190.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4532..4897
                     /gene="yfiL"
                     /locus_tag="ACHKIR_003505"
     CDS             4532..4897
                     /gene="yfiL"
                     /locus_tag="ACHKIR_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HFO9991191.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     NAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKGADENASTIRLN"
     gene            5047..5565
                     /gene="yfiR"
                     /locus_tag="ACHKIR_003506"
     CDS             5047..5565
                     /gene="yfiR"
                     /locus_tag="ACHKIR_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HFO9991192.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSHFSNALQENGTTSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            5555..6781
                     /gene="dgcN"
                     /locus_tag="ACHKIR_003507"
     CDS             5555..6781
                     /gene="dgcN"
                     /locus_tag="ACHKIR_003507"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HFO9991193.1"
                     /translation="MMDNDNSLNKRPTFKRALRNISMTSIFITMMLIWLLLSVTSVLT
                     LKQYAQKNLALTAATMTYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNIL
                     ASWHYTHKEPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSL
                     AVLTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFH
                     RFALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDA
                     RKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEFGGLRHKAYRLGGDEFAMV
                     LYDVQSESEVQQICSALTQIFNRPFDLHNGHQTTMTLSIGYAMTIEHASAEKLQELAD
                     HNMYQAKHQRAEKLVR"
     gene            6797..7279
                     /gene="yfiB"
                     /locus_tag="ACHKIR_003508"
     CDS             6797..7279
                     /gene="yfiB"
                     /locus_tag="ACHKIR_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFO9991194.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     ALSLKRANVVADAWAMGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(7356..7703)
                     /gene="rplS"
                     /locus_tag="ACHKIR_003509"
     CDS             complement(7356..7703)
                     /gene="rplS"
                     /locus_tag="ACHKIR_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFO9991195.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(7745..8512)
                     /gene="trmD"
                     /locus_tag="ACHKIR_003510"
     CDS             complement(7745..8512)
                     /gene="trmD"
                     /locus_tag="ACHKIR_003510"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFO9991196.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(8543..9091)
                     /gene="rimM"
                     /locus_tag="ACHKIR_003511"
     CDS             complement(8543..9091)
                     /gene="rimM"
                     /locus_tag="ACHKIR_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFO9991197.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(9110..9358)
                     /gene="rpsP"
                     /locus_tag="ACHKIR_003512"
     CDS             complement(9110..9358)
                     /gene="rpsP"
                     /locus_tag="ACHKIR_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFO9991198.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(9495..10856)
                     /gene="ffh"
                     /locus_tag="ACHKIR_003513"
     CDS             complement(9495..10856)
                     /gene="ffh"
                     /locus_tag="ACHKIR_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFO9991199.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            11023..11814
                     /gene="ypjD"
                     /locus_tag="ACHKIR_003514"
     CDS             11023..11814
                     /gene="ypjD"
                     /locus_tag="ACHKIR_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HFO9991200.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            11835..13121
                     /gene="yfjD"
                     /locus_tag="ACHKIR_003515"
     CDS             11835..13121
                     /gene="yfjD"
                     /locus_tag="ACHKIR_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HFO9991201.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(13175..13768)
                     /gene="grpE"
                     /locus_tag="ACHKIR_003516"
     CDS             complement(13175..13768)
                     /gene="grpE"
                     /locus_tag="ACHKIR_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFO9991202.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            13891..14769
                     /gene="nadK"
                     /locus_tag="ACHKIR_003517"
     CDS             13891..14769
                     /gene="nadK"
                     /locus_tag="ACHKIR_003517"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HFO9991203.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            14855..16516
                     /gene="recN"
                     /locus_tag="ACHKIR_003518"
     CDS             14855..16516
                     /gene="recN"
                     /locus_tag="ACHKIR_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFO9991204.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKHLA
                     NSGQLLTTSQNALAVMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQQYAEELALLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            16665..17006
                     /gene="bamE"
                     /locus_tag="ACHKIR_003519"
     CDS             16665..17006
                     /gene="bamE"
                     /locus_tag="ACHKIR_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFO9991205.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(17068..17358)
                     /gene="yfjF"
                     /locus_tag="ACHKIR_003520"
     CDS             complement(17068..17358)
                     /gene="yfjF"
                     /locus_tag="ACHKIR_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HFO9991206.1"
                     /translation="MPGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(17348..17785)
                     /gene="ratA"
                     /locus_tag="ACHKIR_003521"
     CDS             complement(17348..17785)
                     /gene="ratA"
                     /locus_tag="ACHKIR_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HFO9991207.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            17956..18438
                     /gene="smpB"
                     /locus_tag="ACHKIR_003522"
     CDS             17956..18438
                     /gene="smpB"
                     /locus_tag="ACHKIR_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFO9991208.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            18653..19015
                     /gene="ssrA"
                     /locus_tag="ACHKIR_003523"
     tmRNA           18653..19015
                     /gene="ssrA"
                     /locus_tag="ACHKIR_003523"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(19176..23750)
                     /gene="ypjA"
                     /locus_tag="ACHKIR_003524"
     CDS             complement(19176..23750)
                     /gene="ypjA"
                     /locus_tag="ACHKIR_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin-like autotransporter YpjA/EhaD"
                     /protein_id="HFO9991209.1"
                     /translation="MNRTSPYYCRRSVLSLLISALIYAPPGMAAFTPDVIGVVNDETV
                     DGSQKVDERGTTNNTHIINHGQQNVYGGISNGSLIESGGYQDVGGHNNFVGQANNTTI
                     NGGRQSIHDGGISTGTIIESGNQDVYTGGISNGTTIKGGNSHISGGTANGTIIDGGGQ
                     TVTTQGHVDGTTINKSGYQDITQGSLATNTTINGGRQYVEQSTVETTTIKNGGEQRVY
                     ESRALDTTIEGGTQSLDSKSTAKNTQIYSGGTQIVDYTSTSDVIEIYSGGVLDVRGGM
                     ATNVTQHDGAALKVTTYDLTVSGTNSEGAFSIHNNVADNVLLENGGHLDINAYGSANK
                     TIIKDKGTMSVLTNAKADATRIDNGGVMDVAGNATNTIINGGTQNINNYGIATGTNIN
                     SGTQNIKSGGKADTTNISSGSRQVVEKDGTATGSNISAGGSLIVYTGGIAHGVNQETG
                     SALVANTGAGTDIEGYNKLSHFTITGGEANYVVLENTGELTVMAKTSAKNTTIDAGGK
                     LIVQKEAKTDSTRLNNGGVLEVQDGGEAKHVEQQSGGALIASTTSGTLIEGTNSYGDA
                     FYIRNSEAKNVVLENAGSLTVVTGSRAVDTIINANGKMDVYGKDVGTVLNSAGTQTIY
                     ASATSDKANIKGGKQTVYGLATDANIESGEQIVDGGSTDKTHINGGTQTVQNYGKAIN
                     TDIVSGLQQIMANGTAEGSIINGGSQVVNEGGLAENSVLNDGGTLDVREKGSATGIQQ
                     SSQGALVATTRATRVTGTRADGVAFSIEQGAANNILLTNGGVLTVESDTTSAKTQVNA
                     GGREIVKTKATATGTTLTGGEQIVEGVANETTINDGGIQTVSANGEAIKTTINEGGTL
                     TVNDNGKATDIVQNSGAALQTSTANGIEISGTHQYGTFSIAGYLATNVLLENGGNLLV
                     LAGTEARDSTVGNGGAMQNLGQDFATKVNSGGQYTLGRSKDEFQVLARAEDLQVAGGT
                     AIVYAGTLADASVSGATGSLSLMTPRDNVTPVKLEGAVRITDSATLTLGNGVDTTLAD
                     LTAASRGSVWLNSNNSCAGTSNCEYRVNSLLLNDGDVYLSAQTAAPATTNGIYNTLTT
                     SELSGSGNFYLHTNVAGSRGDQLVVNNNATGNFKIFVQDTGVSPQSDDAMTLVKTGGG
                     DASFTLGNTGGFVDLGTYEYVLKSDGNSNWNLTNDVKPNPDPNPNPKPDPKPDPKPDP
                     KPDPTPDPTPTPVPEKRITPSTAAVLNMAATLPLVFDAELNSIRERLNIMKASPHNNN
                     VWGTTYNTRNNVTTDAGAGFEQTLTGMTVGIDSRNDIPEGIATLGAFMGYSHTHIFFD
                     RGGHGSVGSYSLGGYASWEHESGFYLDGIVKLNRFESNVAGKMSSGGAANGSYRSNGL
                     GGHIETGMRFTDGNWNLTPYASLTGFTADNPEYHLSNGMESKSVDTRSIYRELGATLS
                     YNMRLGNGMEVEPWLKAAVRKEFVDDNRVKVNNDGNFVNDLSGRRGIYQAGIKASFSS
                     SLSGHLGVGYSHGAGVESPWNAVAGVNWSF"
     gene            complement(23815..23934)
                     /locus_tag="ACHKIR_003525"
     CDS             complement(23815..23934)
                     /locus_tag="ACHKIR_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFO9991210.1"
                     /translation="MPHSYAEYHHFIMMSLMSGEHNTLALSLQDESPGLVPVG"
     gene            complement(24089..24355)
                     /locus_tag="ACHKIR_003526"
     CDS             complement(24089..24355)
                     /locus_tag="ACHKIR_003526"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO9991211.1"
                     /translation="MLRVTTSAHNRGSRPGQNKRLRTLSVGDALLVCKLNRLGRSMWH
                     LVVLLEELCERGINFRALAQSIFAQQWGDGSCKSKTICDLRVIV"
     gene            complement(24686..25780)
                     /locus_tag="ACHKIR_003527"
                     /pseudo
     CDS             complement(24686..25780)
                     /locus_tag="ACHKIR_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961341.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5507 domain-containing protein"
BASE COUNT         6560 a         6721 c         5853 g         6656 t
ORIGIN      
        1 gagtggtaaa ggagaggccc tggaaatacc ccacagcccc gaaaaagagc cagtcagttg
       61 cttaactgat cggcattaca gcccttgagc tgccgttttt ttattctgtt agttgtgaaa
      121 ctgaagcgat ttagtcacta tcgatctcat caaatatggc tcgctttgag atattcctca
      181 agtaaaaaaa cacctcttcc tgcgatttct cacaaaaaag attcgttgac aaaaagtgac
      241 aaaattatga gatttccatc acacattttg acatcaggaa cggtatgctg aattcaccaa
      301 gacgggaaga caagaggtaa aatttatgac aatgaacatt accagcaaac aaatggaaat
      361 tactccggcc atccgccaac atgtcgcaga ccgtctcgcc aaactggaaa aatggcaaac
      421 acatctgatt aatccacata tcattctgtc caaagagcca caagggtttg ttgctgacgc
      481 cacaatcaat acacctaacg gcgttctggt tgccagtggt aaacatgaag atatgtacac
      541 cgcaattaac gaattgatca acaagctgga acggcagctc aataaactgc agcacaaagg
      601 cgaagcacgt cgtgccgcaa catcggtgaa agacgccaac ttcgtcgaag aagttgaaga
      661 agagtagtcc tttatattga gtgtatcgcc aacgcgcctt cgggcgcgtt ttttgttgac
      721 agcgtgaaaa cagtacgggt actgtactaa agtcacttaa ggaaacaaac atgaaacaca
      781 caccgttttt cttcgcattc ttttttacct tcccctgaat gggaggcgtt tcgtcgtgtg
      841 aaacagaatg cgaagacgaa caataaggcc tcccaaatcg gggggccttt tttattgata
      901 acaaaaaggc aacactatga catcggaaaa cccgttactg gcgctgcgag agaaaatcag
      961 cgcgctggat gaaaaattat tagcattact ggcagagcga cgcgaactgg ccgtcgaggt
     1021 gggaaaagcc aaactgctct cgcatcgccc ggtacgtgat attgatcgtg aacgcgattt
     1081 gctggaaaga ttaattacgc tcggtaaagc gcaccatctg gacgcccatt acattactcg
     1141 cctgttccag ctcatcattg aagattccgt attaactcag caggctttgc tccagcaaca
     1201 tctcaataaa attaatccgc actcagcacg catcgctttt ctcggcccca aaggttctta
     1261 ttcccatctt gcggcgcgcc agtatgctgc ccgtcacttt gagcaattca ttgaaagtgg
     1321 ctgcgccaaa tttgccgata tttttaatca ggtggaaacc ggccaggccg actatgccgt
     1381 cgtaccgatt gaaaatacca gctccggtgc cataaatgac gtttacgatc tgctgcaaca
     1441 taccagtttg tcgattgttg gcgagatgac gttaactatc gaccattgtt tgttggtctc
     1501 cggcactact gatttatcca ccatcaatac ggtctacagc catccgcagc cattccagca
     1561 atgcagcaaa ttccttaatc gttatccgca atggaagatt gaatataccg aaagtacgtc
     1621 tgcggcaatg gaaaaggttg cacaggcaaa atcaccgcat gttgctgcgt tgggaagcga
     1681 agctggcggc actttgtacg gtttgcaggt actggagcgt attgaagcga atcagcgaca
     1741 aaacttcacc cgatttgtgg tgttggcacg taaagccatt aacgtgtctg accaggttcc
     1801 agcgaaaacg acgttgttaa tggcgaccgg gcaacaagct ggtgcgctgg ttgaagcgtt
     1861 gctggtactg cgcaaccaca atctgattat gacccgtctg gagtcacgcc cgattcacgg
     1921 taatccatgg gaagagatgt tttatctgga tattcaggcc aatcttgaat cagcggaaat
     1981 gcaaaaagca ttgaaagagt taggggacat cacccgttcg atgaaggtat tgggctgtta
     2041 cccaagtgag aacgtagtgc ctgttgatcc aacctgatga aaaggtgccg gatgatgtga
     2101 atcacccggc actggattat tagtgacggt tgtcattcgc ctgacgcaat aacacgcggc
     2161 tttcactctg aaaacgctgt gcgtaatcgc cgaaccagtg ctccaccttg cggaaactgt
     2221 caataaacgc ctgcttatcg ccctgctcca gcaactcaat cgcctcgccg aaacgcttat
     2281 agtaacgttt gattaacgcc agattacgct ctgacgacat aataatgtcg gcatacagtt
     2341 gcggatcctg ggcaaacagt cgcccaacca tcgccagctc aaggcggtaa atcggcgaag
     2401 aaagcgccag aagttgctca agctgaacat tttcttctgc caggtgcagc ccataagcaa
     2461 aagtagcaaa gtggcgcagt gcctgaataa acgccatatt ctgatcgtgc tcgacagcgc
     2521 taatacgatg cagccgagcg ccccagacct gaatttgctc cagaaaccat tggtatgctt
     2581 ccggcttacg tccatcacac cagaccacaa cttgttttgc caggctaccg ctgtccgggc
     2641 cgaacatcgg gtgtaacccc agcaccggac catcatgcgc cgccagcatg gcctgtaatg
     2701 gtccattttt caccgatgcc agatcgacca gaatacaatc tttcggtaaa ggcggtaatt
     2761 tgccaataac ttgttcagta acgtggatgg gcacactaac aatcaccatt ccggcatcgg
     2821 caactatctc agccgctcga tcccagtcat gttgctccag aatccgcacc tgataacccg
     2881 atagtgtcag catcttctcg aacaggcgtc ccatctgacc gccaccgccg acgataacca
     2941 ccgggcgcag tgacggacaa agcgttttaa atcctttgtc gttttcactg gagtaagatt
     3001 cacgcatcac ccgacgcaaa acatcctcaa tcagatctgg cggtacaccc agagcttccg
     3061 cctctgcacg acgcgaggcc aacatagatg cctcgcgctc cggaacataa ataggcagtc
     3121 caaagcggct tttcacctcg cccacttcag caaccagttc cagacgcttc gctaataaat
     3181 tcagcagcgc tttatcgact tcatcaattt gatcgcgtaa tgcggtcaat tcagcaacca
     3241 taataaacct cttaagccac gcgagccgtc agctgcccgt tcagatcctg atgaatttca
     3301 cgcagcaagg catcggtcat ttcccagcta atgcaggcat cggttacgga tacaccgtat
     3361 ttcatttcac tgcgcggttg ctcggaagac tgattgccct cgtggatatt actttcgatc
     3421 atcagaccaa taattgagcg attgccatct ttgatttgag caaccacgga ttctgccacc
     3481 gcaggctgac ggcgataatc tttattggaa ttaccgtggc tgcaatctac catcagagac
     3541 gggcgcagtc ccgcctgttc catctctttt tcacattgcg caacatccgc agggctatag
     3601 ttcggcgctt taccaccgcg caggatcaca tgcccgtccg gattcccctg agtttgtagc
     3661 aacgccacct gccctgcctg gttaatgcca acaaaacggt gcggctgggc ggcggcacgc
     3721 atagcgttaa tagccgttgc aaggctgccg tcggtgccgt ttttaaaacc aaccggcatg
     3781 gaaagcccgg aggccatttc acggtgtgtt tgcgattccg ttgtacgagc accaattgct
     3841 gaccagctaa acagatcgcc caggtattgc gggctattcg gatctaacgc ttccgtcgcc
     3901 agtggcagtc ccatattcac cagctcaagc agcaatttac gcgcgatctg cagcccggct
     3961 tctacatcaa aagagccatc catatgggga tcgttaatca accctttcca accgacagtg
     4021 gtacggggtt tttcaaaata gacgcgcatt accagataga ggctatcgct gacctctgcg
     4081 gcaagggctt taaatcgacg agcatattcc agagcagttt ccggatcatg aatggaacaa
     4141 ggaccacata ctaccaacag acgaggatcg cgcccggcga taatatctga aatggtttta
     4201 cgcgagtcag caatctgggc ttcctgttgc aggcttaatg gaaaagcggc cttcagttgt
     4261 tccggagtca ttaaaacttg ttcgtcggta atatgtacgt tattcagcgc gtctttttgc
     4321 atgatggcga tcctgtttat gctcgtttgc gatagttgat cctcagcgag gatgacgtaa
     4381 cgataacaca taaagtaaag ttttcaatcc atatttcgta catttttctt tacacaagca
     4441 atttattcgc gcttttaacc cttgtttatg tataaataaa tttacaccac ctttgaaaac
     4501 aatccgctat gctttgaaaa aggagaaaga aatgatgaaa aagtttatcg cccccttgtt
     4561 ggctttactg gttagcggat gtcagattga tccttatact cacgcgccaa ccttgaccag
     4621 caccgactgg tatgatgttg gtatggaaga tgcgatatcg ggcaacgcca taaaagatga
     4681 cgatgcattt agcgattcac aggcggatcg cggtctatac cttaaaggat atgccgaagg
     4741 acaaaagaaa acttgccaga ccgattttac ttatgcccga ggactttccg gtaaaagctt
     4801 tcctgccagc tgcaacaatg ttgaaaatgc cagccaactg catgaagtat ggcaaaaagg
     4861 ggctgatgaa aatgccagca caatacgatt aaattaatta aacccccaca aataaattaa
     4921 acatgagatt ctactcattg tgttttattt ctcacattga tgacgaccgc atgaaagaat
     4981 gatattttaa tgagcattct gaaaaaatat cgatgcattt cgagcgaaga tggtgaggat
     5041 ccctgaatgc gtttttctca ccgactgttc ttactgctga ttctcctgct gacgggagca
     5101 ccaatactcg ctcaggaacc ttctgacgtt gcgaaaaacg tgcgtatgat ggtttccggt
     5161 attgtcagtt acacccgctg gcctgcgcta tcagggccac caaagttatg tatcttttca
     5221 tcttcacatt ttagtaacgc cctacaagag aatggcacga catctttgcc ttatctacct
     5281 gtcatcattc acactcaaca agaggcgatg atttcaggct gcaatggttt ttattttggc
     5341 aacgagtcac cgacattcca gatggaatta accgaacaat atccgtcaaa ggcgttatta
     5401 ttaattgccg aacaaaatac cgagtgcatt attggtagcg ccttttgtct aatcatccac
     5461 aataatgacg tcagatttgc cgtaaacctg gatgccttat cgcgtagcgg tgtaaaggtc
     5521 aacccggatg tcttaatgct cgcacggaag aaaaatgatg gataacgata attctcttaa
     5581 taagcgcccc acgtttaaaa gagcattacg caacatcagt atgaccagca tatttatcac
     5641 tatgatgctg atctggttgc tgctttccgt gacctcggtg ctgaccctga aacagtacgc
     5701 gcaaaaaaac ctggcactga cagcagcaac aatgacttac agtctggaag cagctgtcgt
     5761 ttttgccgat ggccctgcag caactgaaac actggcagcg ctgggccagc aaggccaatt
     5821 ttcaactgca gaagtgcgtg ataagcagca aaatattctg gcatcctggc attacaccca
     5881 taaggaacca ggcgatactt tcagcaattt cataagccac tggctcttcc ctgcccccat
     5941 cattcagccg attcgtcaca atggtgaaac cattggcgaa gtacgcttaa ctgctcgcga
     6001 cagttcaatc agccatttta tctggttttc gctcgctgtc ctgaccggtt gtattctgct
     6061 ggcatcaggc atcgcaatta ccctcacccg ccatttgcac aatggcctgg tggaagcgct
     6121 gaaaaatatc accgatgtcg tacatgatgt gcgttctaac cgcaattttt cccgacgagt
     6181 ttcggaagaa cgtatcgctg agtttcaccg cttcgctctc gacttcaaca gtctgctgga
     6241 tgaaatggaa gagtggcagc ttcgtttaca ggccaaaaat gcgcagcttt tacgtaccgc
     6301 gctacatgac ccattaaccg ggctggctaa ccgcgcagcg tttcgtagcg gcatcaacac
     6361 gttgatgaac aattccgatg cccgaaaaac gtcggcgtta ctatttcttg atggcgataa
     6421 tttcaaatat atcaatgata cctggggtca tgcgacgggc gatagagtct tgattgaaat
     6481 cgcaaaacgg ttagctgaat ttggcggact gcgacataaa gcataccgcc tgggcggcga
     6541 tgaattcgcg atggtgctct atgatgtaca gtcggaatct gaagtgcagc agatatgctc
     6601 agcactgaca caaatcttta atcgcccgtt tgatcttcat aatggtcatc agaccaccat
     6661 gacattaagc attggttacg cgatgaccat tgagcatgcc tctgcggaaa aattacaaga
     6721 gctggccgat cacaatatgt atcaggccaa acaccagcgt gccgaaaagc tggtgagata
     6781 acaaggatat atcgatatga taaagcacct ggtagcaccc ctgattttca cctcactaat
     6841 actgactggc tgccagtccc ctcagggaaa gtttactcct gagcaagtcg ccgctatgca
     6901 atcttatgga tttactgaat ccgccggcga ctggtcgctg ggcttatcag atgccattct
     6961 gttcgcaaaa aatgactaca aattgctccc ggaaagccag caacagatcc aaaccatggc
     7021 agccaaattg gcctcgacag ggctaacgca tgcccgtatg gatggacaca ccgacaacta
     7081 tggtgaagac agttacaacg aagccttatc attgaaacgg gcgaatgtcg tggccgatgc
     7141 atgggctatg ggtggacaaa ttccgcgcag caatctcacc acacagggtt taggaaaaaa
     7201 atatcccata gccagtaaca agaccgccca gggccgcgcc gagaaccgcc gcgtcgcagt
     7261 ggtgattact accccttaaa acacgagcaa acagataaaa aaagggccag ccaattggcc
     7321 agcccttctt aacaggatgt cgcttaagcg aaatcttagt taagacgctc tttgatacga
     7381 gcagccttac cagtacgctc acgcaggtag tacagtttag ctttacgaac agcaccacga
     7441 cgtttgacag aaatgctgtc aactaccgga gagtgagtct ggaagacacg ctcaacgcct
     7501 tcgccgttgg aaattttacg aacagtgaat gcagagtgca gaccgcggtt acgaatagcg
     7561 ataaccacgc cctcgaatgc ctgcagacgt tttttggaac cttcaacaac ccatactttc
     7621 acttccacgg tatcacccgg acggaaggaa ggtacgtcct gcttcatctg ctcttgttca
     7681 agttgcttaa taatgttgct cataatttaa tctcttatcc tgggtaaact gatatctcgg
     7741 gggcttacgc catcccatca tgtttatgtt gctgttgtgc gtgttccgtt ttgaactccg
     7801 ccagcaacct tgcttgctct tcagtcagag ccaggttttc cagaagttca ggtcttctaa
     7861 gccaggtacg gcccagcgac tgtttcaaac gccagcgacg tatctcggca tggttgcccg
     7921 acagtaacac tggcggaact tccatccctt ctaacacctc aggccgcgta tagtgcgggc
     7981 aatccagcaa tccttcagca aaggaatctt ccgttgccga ggcttcatgt cccagtactc
     8041 ccggaataaa ccgggaaacg gagtcaatca gcgtcattgc tggtaactca ccaccactga
     8101 gaacgtaatc gccgattgac cattcttcgt caatttcggt ttggatcacg cgctcatcta
     8161 taccttcgta gcgaccgcac accagaatca atttttgatt cgttgccagt tcgctgacgc
     8221 ccgcttgatc aagcttgcgt ccctgtggtg acagataaat cacctttgcg ccttcacccg
     8281 ccgcggcttt tgctgcatga atggcgtccc gcaagggttg caccatcatt aacatccccg
     8341 gtccgccgcc gtaaggacga tcgtccacgg tacggtgccg gtcatgcgtg aagtcgcgag
     8401 gactccagct ctggatgctc agcaggccat ttttaactgc ccggccagtt accccgtaat
     8461 cggtaattgc gcggaacatt tcaggaaaca ggctaattat gccaatccac atagcgccgt
     8521 cttttaccgt ttatccggtg gtttaaaaac caggatccca atctacttcg attgaacgag
     8581 tagtgagatc gactttcttg ataacctgcc catcgaggaa cggtacgaga cgttccttga
     8641 taccaaacgc atctttcagg tttgccttaa tgacgagaac gtcattagat ccggtttcca
     8701 tcatatcgac gactttaccg agatcgtagc cttcagtggt tactacctgg cagcccatca
     8761 agtctttcca gtagtagtca ccctcttcaa gctgaggcaa ctgcgatgaa tccacgacaa
     8821 tttcacaatt cgtcagcagg ttcgccgcat cacgatcgtc aacgcctttc agcttgatga
     8881 tcatgtcctg attgtggtgc ttccagcttt ccagctggac ttgctgccac tgacccgcct
     8941 tctggataaa ccagggctga tagtcaaaaa tgctttcggc gtcttcggtg gaagaaaaca
     9001 ctctgagcca cccacgaata ccgtaagacg aacccatttt tcccaaaacg atgggatcaa
     9061 caggtgcttg cgcggtgagt tgtttgctca tcatgaccac cgtgacagat taagctgctt
     9121 tgtttacttc tttgatcagc gcagcaacgc gatcagaaat agttgcgccc tggccaaccc
     9181 agtgagcgat gcgatccaga tccaggcgag tgccttcttc tttttcgcta gcgattgggt
     9241 tgaagaaacc aacgcgctcg atgaagcgac cgttgcgtgc attacggctg tcagcgacaa
     9301 caacctggta gaacggacgc tttttagcgc cgtgacgtgc taaacgaata gttaccataa
     9361 catcctcttg tgtgaataaa acaaccggac cccatcgagg aacggagtcc ggtgtcatat
     9421 taaaagcccg aaaattttac tgatttctgg agaaaatgca atcagcagtt aataactctg
     9481 tcgcataagg tcatttagcg accaggaaat cccggcggca tcataccctt catgcttctc
     9541 atcatcttcg ccattccgcc cttcttcatt ttcttcatca tgcgctgcat gtcgtcgaac
     9601 tgtttcagaa gacggttaac gtcctgcacc tgcataccgc aaccggcagc gatacggcgt
     9661 ttacgcgaac ctttgatgat ttctggctta gcgcgctctt tcatcgtcat cgagttgatg
     9721 atggcttcca tacgcaccag cactttatcg tccatctgcg acttgacgtt atccgggatc
     9781 tgccccatgc ccggcagttt gcccatcagg ctggccatgc cgcccatatt tttcatctgg
     9841 cgcagttgct caaggaagtc attgagatcg aagccgtcgc cttttttcag cttgctggct
     9901 aatttctcag cttgcgcgcg gtcaactttg ctttcgatat cttcgatcag cgacagtacg
     9961 tcgcccatgc cgagaatacg ggaagcgatg cggtccggat ggaacggctc cagcgcctcg
    10021 gttttctcgc caacaccgag gaacttgatc ggtttgcccg taatgtgacg aatagagagc
    10081 gccgcaccgc cacgagcatc gccgtcgact ttagtcagta ccacaccggt cagcggtaac
    10141 gcttcattga atgcttttgc cgtattggcc gcatcctgac cagtcatggc atcaaccaca
    10201 aacagggttt caaccggatt aatcgacgca tggacttgtt tgatctcgtc catcatcgct
    10261 tcgtcaacgt gcagacgacc ggctgtatcc accagcagca cgtcgtagaa tttcagtttg
    10321 gcttctttca gcgccgcgtt aacgatatct accggcttct gaccaacatc agaagggaag
    10381 aaatcaacgc ccacctgctc tgccagcgtc tcaagctgtt tgattgccgc cggacgataa
    10441 acgtcggcag aaaccaccag cactttcttc ttgtgcttct cgcgcaggaa cttaccgagt
    10501 ttaccaacgc tggttgtttt accggcacct tgcaggcccg ccatcagcac gaccgcaggc
    10561 ggttgcgcag ccaggttcag ggtctggttc tcttcgccca tcgccgcaac cagttcgtta
    10621 cggacaattt tgacgaactc ctgccccggc gtcaggctct tattaacttc atgaccaacc
    10681 gctttctctt ttacgcgatt gataaactca cgcactaccg gcagagcgac gtccgcctcc
    10741 agcagcgcca tgcgcacttc gcgcagcgta tcttttacgt tgtcttcagt gaggcgtcca
    10801 cggccactga tattgcgcag cgtgcgcgac aaacgatcgg ttaaattatc aaacattgtc
    10861 tctcgcctgg ggtggaaacg gttggccgca atcgcgacac atcatcagta ttttgccgca
    10921 gtataacatg aaggcgtctt tgttgttatg caacggttgg agcagcgttc acctgacgct
    10981 atactgcttc tctttcttat tgctcaaact gtcgacatta ctatgcccgt ttttgctctg
    11041 ctcgcgcttg tcgcctactc cgtcagtctt gcgctgattg ttcccggtct gctgcaaaaa
    11101 aacggcggct ggcggcgcat ggctattatt tctgcggtca ttgcgctggt ctgccacgca
    11161 atcgctctgg aagcccgcat cctgcccgac ggtgatagcg gacaaaacct cagcctgctg
    11221 aacgttggtt cattggtcag tttgatgatc tgtacggtaa tgaccattgt ggcttctcgc
    11281 aatcgtggct ggttgctgct acccattgtc tatgcctttg cgcttatcaa cctggcgctg
    11341 gcgaccttca tgcccaatga atacatcacc catctggaag caacgcctgg gatgctggtg
    11401 cacattggct tatcgctctt ttcctatgcc acgctgatta tcgccgccct gtacgcgctg
    11461 caactggcgt ggattgatta ccaactgaag aacaagaagc tggcgtttaa ccaggaaatg
    11521 ccgccattga tgagtatcga gcgtaaaatg ttccacatca cgcagattgg cgtggtgctg
    11581 ctaacgctca cgctttgcac tggcctgttc tacatgcaca acttatttag catggaaaat
    11641 atcgacaagg ccgtgctctc tatcgtggcg tggtttgtct atattgtgct gctgtgggga
    11701 cattatcatg aaggatggcg tggacgccgc gtcgtctggt ttaacgttgc gggtgcagtc
    11761 attctgacac tggcctactt cggcagccga attgtccagc agttaatcag ctaaacccag
    11821 aaaaggagtt tcccctggaa cacatttcta ctactacgtt gatcattatt ctgatcatca
    11881 tggtggtcat ttcagcctat ttttccgggt ccgaaaccgg aatgatgacc ctcaaccgct
    11941 atcgtctgcg acatatggcg aaacagggta atcgctcggc caaacgcgtc gaaaaattgc
    12001 tgcgtaagcc agaccgcctg attagcctgg tgttaatcgg caataacctg gtcaatattc
    12061 ttgcctccgc gctaggcact attgttggga tgcgtttgta cggcgatgcg ggcgtggcaa
    12121 ttgcgactgg tgtgctgact tttgtcgtgc tggtttttgc tgaggtattg ccgaaaacca
    12181 ttgccgcgct gtacccggaa aaagtcgctt atccgagtag ttttctgctg gctccgctgc
    12241 aaattttgat gatgccgctg gtctggttgc tgaatgctat cacccgtatg ctgatgcgca
    12301 tgatgggtat caaaaccgat atcgtggtta gcggctcttt gagcaaagaa gagttgcgca
    12361 ctatcgtgca cgaatcgcgc tcacaaattt cccgtcgcaa tcaggatatg ctgctgtcgg
    12421 tgctcgatct ggaaaaaatg accgttgatg acatcatggt gccgcgcagt gaaattatcg
    12481 gtattgatat caacgatgac tggaaatcga ttctgcgcca actctcccac tcacctcacg
    12541 ggcgcatcgt gctctaccgt gattcgctgg acgacgccat cagtatgctg cgtgtgcgtg
    12601 aagcctggcg actcatgtcg gagaaaaaag agttcaccaa agaaaccatg ctacgcgccg
    12661 cggacgagat ctattttgtc ccggaaggta cgccgctcag cacgcagttg gtgaagtttc
    12721 agcgcaacaa aaagaaagtc ggcctggtcg tcaacgagta tggagacatt caggggctgg
    12781 tgacggttga agatattctg gaagagattg tcggcgattt cactacctcg atgtcgccaa
    12841 cacttgccga agaggtcacg ccgcaaaacg acggttcggt gattatcgat ggcaccgcca
    12901 acgtgcgaga aatcaacaaa gcctttaact ggcatctacc tgaagacgat gcccgcacgg
    12961 ttaatggcgt cattctggaa gcgctggaag agatccctgt cgctggcacc cgcgtgcgta
    13021 ttggcgagta cgatatcgat attctcgacg tacaggataa tatgattaag caggtaaaag
    13081 tttttcctgt gaaaccgctg cgcgagagtg tggcggagta aaaaaacggc ccggcattcg
    13141 catgctgggc cgtgatttat tacgaaagca gaaattaagc ttttgccttc gctacagtca
    13201 ccatcgccgc acgaatcgta cgaccgttca gcgtataacc tttctgcata atgcccagta
    13261 cgttacctgg cgcaacgtca tcagattcca ccattgcgat ggcctgatgc acatttgggt
    13321 ccagtgggac gttggtttcg gcgatcactt caacgccaaa cttacgcaca acatccagca
    13381 tcgacttcag cgtcagctca ataccttcaa ccatcgcgga catatccggg ttagctttat
    13441 cagccacttc cagcgcacga tccaggctat caatcaccgg cagcaattcg ttgatgaatt
    13501 tctccagcgc gaatttgtga gctttttcaa tatccagttc agtacgacga cgcaggtttt
    13561 ccatttcggc ttttacgcgc aaaatgccgt cacgttcacg ggtctgggct tcagccagct
    13621 gagcttcgag attcgcaatt ttttcatcgc gcggatccac ctgctcagca gaagcttctg
    13681 gctcaactgc ctcaatctct tcgtgctgat ccatgataat ttcttccggg gcttgcccct
    13741 caggcgtttt ctgttcttta ctactcatga atttctccgc gtttttttcg cattcatctc
    13801 gctaacttcg cttattatgg ggatcagttt cagggtttca agggaagcac tcacattgtc
    13861 atcaatcttc gcaacaagga cctcggaaaa atgaataatc atttcaagtg tattggcatt
    13921 gtgggacacc cacggcaccc cactgcactg acaacacatg aaatgctcta ccgctggctg
    13981 tgcacaaaag gttacgaggt catcgttgag caacaaatcg ctcacgaact gcaactgaag
    14041 aatgtgaaaa ctggcacgct cgcggagatt gggcaactgg cagatctcgc ggtagtcgtt
    14101 ggtggcgacg gtaatatgct gggcgcggcg cgcacactcg cccgttacga tattaaagtt
    14161 attggaatca accgtggcaa cctgggtttc ctgactgacc ttgaccccga taacgcccag
    14221 caacaattag ccgatgtgct ggaaggccac tacatcagcg agaaacgttt tttgctggaa
    14281 gcgcaagttt gtcagcaaga ttgccagaaa cgcatcagca ccgcgattaa cgaagtggta
    14341 cttcatcctg gcaaagtggc gcatatgatt gagttcgaag tgtatatcga cgagatcttt
    14401 gcgttttcgc agcgttccga tggactgatt atttcgacgc caacaggctc caccgcatat
    14461 tccctctctg cgggcggtcc tattctgacc ccctctctgg atgcgattac cctggtgccc
    14521 atgttcccgc atacgttgtc agcacgacca ctggtgataa acagcagcag cacgatccgt
    14581 ctgcgttttt cgcatcgccg taacgacctg gaaatcagtt gcgacagcca gatagcactg
    14641 ccgattcagg aaggtgaaga tgtcctgatt cgtcgctgtg attaccatct gaatctgatt
    14701 catccgaaag attacagtta tttcaataca ttaagcacca agctcggctg gtcaaaaaaa
    14761 ttattctaat tttacgccag cctctttact gtatataaaa ccagtttata ctgtacacaa
    14821 taacagtaat ggtttttcat acaggaaaac aactatgttg gcacaactga ccatcagcaa
    14881 ctttgctatc gttcgtgagc ttgagattga ttttcatagc ggcatgaccg taataactgg
    14941 cgagaccggc gcgggtaaat ctattgcaat agatgccctc ggcctttgtc tcggtggtcg
    15001 cgctgaagcc gacatggtgc gtaccggcgc tgctcgtgcc gacctgtgcg cccgtttttc
    15061 tctgaaagat acgccagcgg ccctgcgctg gctggaagaa aaccagcttg aagacggtca
    15121 tgaatgtttg cttcgtcgcg taatcagtag cgatggtcgc tcacgaggtt ttatcaacgg
    15181 tacagctgtt cctctgtcac aacttcgcga actgggccag ttgctgattc agatccatgg
    15241 tcagcacgcc catcagttac tcaccaaacc cgagcaccaa aaattcctgc ttgatggcta
    15301 tgccaatgaa acctctctgc tccaggaaat gaccgcacgt tatcagttgt ggcatcaaag
    15361 ctgccgtgac ctcgcgcatc atcaacagtt aagtcaggaa cgcgccgccc gtgcggaact
    15421 gctgcaatac caattaaaag aacttaacga atttaatccg cagcccggag agtttgagca
    15481 aatcgacgaa gagtacaaac atctggcgaa cagcggtcaa ttgctgacta ccagccagaa
    15541 tgcattggca gtaatggccg acggtgaaga cgcaaacctg caaagtcagc tttacacggc
    15601 taaacaactg gtgagcgaat tgattggcat ggacagcaaa ctgtccggcg tacttgatat
    15661 gctggaagaa gctaccatcc agattgctga agccagcgat gaactgcgcc actactgcga
    15721 tcgtctggat ctcgatccca accgactgtt tgaacttgaa cagcgcatct caaaacagat
    15781 ttcgctggca cgtaaacatc acgtcagccc tgaggcattg ccacagtatt accagtcgct
    15841 actggaagaa cagcagcaac tggacgatca ggccgactca caagaaacgc tcgcgctggc
    15901 ggtaacgaaa catcatcagc aggcactgga aaccgcgcgc gcattacacc agcaacgcca
    15961 gcagtatgca gaagaacttg cactgctgat caccgacagt atgcatgcgc tctcaatgcc
    16021 gcatgggcag tttacgatcg atgttaaatt tgacgagcat cacctgggcg ctgacggtgc
    16081 cgatcgtatt gagtttcggg taaccaccaa cccaggtcag ccaatgcagc ctattgccaa
    16141 agtcgcatcc ggtggtgaat tgtcacgcat cgcattggca attcaggtca tcacggcgcg
    16201 taaaatggaa accccggcac tgatttttga tgaagtggat gtagggatta gcggtccaac
    16261 agcggcagtt gtcggcaaac tgctgcgtca acttggcgaa tcaactcagg tgatgtgtgt
    16321 tacccacctg ccacaagtcg cgggatgtgg tcatcaacac tattttgtca gcaaagaaac
    16381 cgatggtgcg atgacagaaa cacacatgca atccctggat aaaaaagcgc ggttacaaga
    16441 gctggcgcgc ctgcttgggg gcagtgaagt cacacgtaac acactggcga atgcgaaaga
    16501 actgcttgcg gcgtaaactt ttttcctgct tcacggtcag agtaaacagc aaaacgccgt
    16561 aagaccggaa agcaaaaggt tttaaagtga tgaaaggtct attatcatcg gcatattaca
    16621 gatgagccac gtactgctcg ggcccgaaaa ggaatcaaat cactatgcgc tgtaaaacgc
    16681 tgactgctgc agcagcagta ctattgatgt tgaccgcagg ctgttccact ctggagcgag
    16741 tggtttaccg tcctgacatc aaccagggga attatctgac cgctaacgac gtatccaaaa
    16801 tacgcgttgg catgacgcaa caacaagttg cgtacgcatt gggtacaccg ctgatgtccg
    16861 atccatttgg tacgaatacc tggttctatg tcttccgcca gcaaccaggt catgaaggtg
    16921 taacgcagca aacgctgacg ctgaccttta atagtagcgg ggtgttgacc aatattgata
    16981 ataaacctgc gctgagtggt aactaatcat aaagttgctc tcaaagacgt taaaaaaggt
    17041 gctcaatgag cacctttttt ctgtctgtta tttattcgct gatttttctg ctcgttgcct
    17101 gcgcagctct ttcggatcgg caatgagagg gcgataaatc tccacccgat cgccatcatg
    17161 cacactatcg ctcagttttg ccggacggct ataaatgccg actttgtttt tagttaaatc
    17221 gatatcggta cgcaattcca gcaagccact ggcgcgaata gcttcttcaa ccgtcgcacc
    17281 ctcctgcagc gtcacacgct gcaggtactg cttctcaggt agcgcataag ccacctcaac
    17341 ggcaatttta cctggcactg taaacctctt tcgcacgaac cgtaaaagcc tggaccatat
    17401 tagccgccag ttctttaaac acgcggccaa aggcgagttc aatcaactta ttggtaaact
    17461 caaaatcgag atgaaactcg atacggcacg cctcctgact cagcggcgta aacttccatc
    17521 caccgatcaa tttcttgaac ggcccatcca ccagattcat aagaatactt tggttactgg
    17581 tcaactggtt gcgggtagta aacgttttgc tgatcccagc cttagagaca tctaccgccg
    17641 cagtcatctg cccaggagtg gactccagaa tccgacttcc ggtacaaccc ggcaaaaact
    17701 gaggataaga ctgaacgtca ttcactaact gatacatttg ctccgcgctg tagggtacca
    17761 gtgcggtccg gctaatctga ggcataacaa tttccatcaa caaaaatcca acaaataata
    17821 tcatttatcc tgctgtaaaa aaaacgctat cccggcgctg ggtaacatcg ggttcatgct
    17881 aagatagagc cttgtccccc gcaggattga tatggggtgt tttcgatttc agattaccga
    17941 tgattcacga cgcttatgac gaagaaaaaa gcacataaac ctggttcagc gaccatcgcg
    18001 cttaacaagc gcgcccggca cgaatacttt atcgaagaag agttcgaggc gggacttgcc
    18061 ctgcaaggct gggaggttaa atccctgcgt gcaggaaaag ccaatatcag cgacagctac
    18121 gtccttctgc gtgacggaga ggcatttctg tttggcgcta acatcacgcc aatggctgtg
    18181 gcctccacgc atgtggtgtg cgatcctacc cgtacccgca agttacttct caaccagcgc
    18241 gaactggact cattgtacgg tcgcgtcaat cgagaaggct ataccgtagt ggcgctctcc
    18301 ctgtactgga aaaatgcctg gtgcaaagtg aaaatcggcg tcgccaaagg taagaaacag
    18361 catgataaac gttcagatat caaagaacgc gaatggcagg tggataaagc gcgtatcatg
    18421 aaaaacgccc accgttaaac ctgcactgca attattgatc cgctcctcac cgcgcctccc
    18481 tctccggcgg cgcgaatgca catcttattg gctatcacat ccgacccaaa tgttgccatc
    18541 ccattgctta atcgaataaa aatcaggcta catgggtgct aaatctttaa cgataacgcc
    18601 attgaggctg gtcatggcac tcatgaatct ggtatactta ccgttacaca ttggggctga
    18661 ttctggattc gacgggattt gcgaaaccca aggtgcatgc cgaggggcgg ttggcctcgt
    18721 aaaaagccgc aaaaaatagt cgcaaacgac gaaaactacg ctttagcagc ttaataacct
    18781 gcttagagcc ctctctccct agcctccgct cttaggacgg ggatcaagag aggtcaaacc
    18841 caaaagagat cgcgtggaag ccctgcctgg ggttgaagcg ttaaaactta atcaggctag
    18901 tttgttagtg gcgtgtccgt ccgcagctgg caagcgaatg taaagactga ctaagcatgt
    18961 agtaccgagg acgtaggaat ttcggacgcg ggttcaactc ccgccagctc caccaatcat
    19021 gattggacgg tgtaaggaca acaccaacaa aaacaggaag ttagcagtct cagcaagaca
    19081 ccgaccagac ggtgaggaga cataaaagga tacgcaaagg agccgcggct cctggtgata
    19141 tgaaagccca cagatgtggg ctttttcgtt gatggtcaga acgaccagtt cacaccagcc
    19201 accgcgttcc acggggattc cacaccggca ccatggctat accccacccc gagatgcccg
    19261 cttaacgaac tgctgaatga ggctttaata cctgcctggt atattccacg tctgcccgac
    19321 aaatcattga caaaattacc gtcattattc actttcaccc ggttatcatc gacaaattct
    19381 ttgcgcacag ccgccttcag ccacggctca acttccatgc cgttccccag acgcatgttg
    19441 taactcagcg ttgcgcccag ttcacgatat atactacggg tatcgactga tttcgattcc
    19501 atgccattgg ataaatgata ttcggggtta tcagcggtga accccgttaa cgatgcatac
    19561 ggcgtcaggt tccagttacc atcggtaaat cgcatcccgg tttcaatgtg accgcccagc
    19621 ccgttgctgc gataactgcc gttggcggct ccaccgctgc tcattttacc ggctacgtta
    19681 ctttcaaagc ggttcagctt cacgataccg tccagataga aaccactttc atgttcccag
    19741 ctggcatagc cccccagaga ataactgccc acactgccat gccctccacg atcaaaaaag
    19801 atatgtgtat gggaataacc cataaaagcg cccagcgtcg caatcccctc aggaatatca
    19861 ttacggctat cgatccccac cgtcattccg gtcagcgtct gctcaaaccc ggcacccgca
    19921 tcggtggtga cattattacg ggtgttatac gtcgtccccc agacattatt gttgtgtgga
    19981 ctcgctttca ttatgttcaa ccgctcgcga atactgttta gctcagcatc aaagaccaac
    20041 ggtaatgttg ctgccatatt gagtacggct gccgtagaag gcgtgatgcg tttctccggg
    20101 acgggtgtcg gcgttggatc gggagtcggg tctggtttcg gatccggttt tgggtctggt
    20161 tttggatccg gcttcggatt tgggttgggg tccgggttgg gtttgacatc attggtcagg
    20221 ttccagttgc tgttgccatc gcttttcagg acatactcat aggtcccaag atcaacgaaa
    20281 ccgccggtat tacccagcgt aaacgaagca tcccctcccc ctgttttcac cagcgtcatc
    20341 gcgtcgtcag actgagggct gacgccggta tcctgaacaa agattttaaa attgccagtg
    20401 gcgttgttgt tgacgaccag ttgatcgccc cgggagcctg caacgttggt atgcaggtag
    20461 aaattaccgc tgccggaaag ttcactggtg gtcagcgtat tgtagatacc attagttgtg
    20521 gcaggcgctg ctgtttgtgc tgataaataa acatcaccgt cgttgaggag caaactattt
    20581 actctatatt cgcagttgct ggtgcctgca caggaattat tgctgttaag ccagacactg
    20641 ccccggctgg cagccgtcag gtcggcaagc gtcgtatcga caccatttcc cagagttaat
    20701 gtcgcgctat cggtaatccg gaccgcccct tcaagtttta ctggcgtaac attatcccgt
    20761 ggcgtcatta acgacaggct tcctgtcgcg ccactgaccg atgcatccgc cagcgtacct
    20821 gcgtagacga ttgccgtccc gccagcaacc tgtagatctt ctgcccgggc cagaacctga
    20881 aactcatctt ttgaccgacc gagggtatat tgcccaccag aattaacctt tgtggcgaag
    20941 tcctgcccca ggttttgcat tgccccaccg ttgccaaccg tggaatcgcg agcttcggta
    21001 cctgctaata ccaataaatt accgccattt tccagcagta cattggtcgc taaatatccg
    21061 gcaatggaaa aggtgccgta ctggtgcgta ccgctgattt caataccgtt agccgtgctc
    21121 gtctggagag cggcaccgct gttctggacg atatctgtcg ctttgccatt atcgttgact
    21181 gtcagcgttc cgccttcatt gatcgttgtt tttattgcct ctccgttagc tgaaactgtt
    21241 tgtattccgc cgtcgttaat tgttgtctca tttgccacac cctcgacgat ttgttcaccg
    21301 ccagtgagcg tcgtgccagt cgcagtggct tttgttttga cgatctcccg accgcccgca
    21361 ttgacctgtg ttttggcgga agtggtgtct gactccacgg ttaacacgcc gccatttgtc
    21421 agcaggatat tgttcgccgc accctgctcg atgctgaacg cgacgccatc agcgcgtgtc
    21481 cctgtgaccc gcgtcgccct ggtggttgca accaacgcgc cctgactact ctgctgtatc
    21541 cccgttgcgc tgcctttctc ccgcacatcg agtgtgccgc cgtcattaag caccgagttt
    21601 tcagccagac cgccctcatt aactacctgt gaaccgccat taataatgga accttccgct
    21661 gtcccgtttg ccataatttg ttgtaagcca gagacgatat cggtattgat cgccttacca
    21721 taattctgaa cggtttgtgt gccgccattg atgtgcgttt tgtctgttga cccaccatca
    21781 acaatttgtt caccactttc aatatttgca tcagtggcta aaccatatac cgtttgcttg
    21841 ccacctttga tatttgcttt atcagaagtg gcactggcat atattgtttg ggtgcccgca
    21901 ctattgagta cagtgccaac atcttttcca taaacatcca ttttgccgtt ggcattaata
    21961 atcgtatcaa ctgcccggga accagtgacg actgttaatg agccagcgtt ttccagcact
    22021 acatttttag cttctgaatt cctgatgtag aaagcatcac cataactgtt ggttccttcg
    22081 ataagggttc cggaagtcgt ggaagcaatt aatgcgccgc cggattgttg ctcaacatgc
    22141 ttagcctcac caccgtcctg aacctccaga acgccaccat tattaagtct ggtgctatct
    22201 gttttagcct ccttctggac aatcagctta ccgccagcat caatggtagt atttttcgcc
    22261 gaggttttag ccattaccgt cagttcgccg gtattttcca gcacaacata attagcctca
    22321 cctccggtaa tagtgaagtg agagagctta ttgtatcctt cgatatcagt ccctgcgccc
    22381 gtgttggcaa ctaaagcact gcccgtctcc tggttaaccc catgtgcaat accgccggta
    22441 tagacaatca gcgagcctcc ggcgctaata ttgctgccag ttgccgtacc atctttctca
    22501 acaacctgcc ggctcccaga ggatatattt gtcgtgtcag ctttcccgcc gctcttaata
    22561 ttttgtgttc cgctgttgat attggtgccc gtggcaatac cataattatt aatattctgt
    22621 gtgccaccat taattatggt atttgtcgcg tttccggcaa catccataac gccgccatta
    22681 tctattcggg tcgcatcagc tttagcattc gttaaaactg acattgttcc tttatcttta
    22741 ataatcgtct tatttgccga accatatgcg tttatgtcta aatgaccacc gttttccagc
    22801 aacacattgt ctgccacgtt attgtggatg gagaatgccc cttcactatt cgtaccgctc
    22861 accgtcaaat cgtaagtcgt tactttcaaa gcggctccat cgtgctgagt aacatttgtt
    22921 gccataccac ctctaacatc aagcacgcca ccggaataaa tttcaataac atccgaggtg
    22981 ctagtgtaat caacaatttg cgtaccacca gaatagatct gagtattttt tgccgttgac
    23041 ttactatcca gagactgagt tccaccttca atcgtcgtgt ccagcgcacg gctctcatat
    23101 actctttgct caccgccatt tttaatggtg gttgtttcta ctgtgctctg ttcaacatac
    23161 tgtcgaccac catttatggt tgtgttcgtc gccagacttc cttgcgtgat gtcctgataa
    23221 ccggacttat ttatcgttgt accatcaaca tgcccctgag ttgttactgt ctgccctcca
    23281 ccatcaatga tggttccatt cgcagtcccc ccacttatgt gtgaattacc acccttaatt
    23341 gtcgttccat tgctgatacc acctgtataa acgtcctgat tgccactctc gattattgta
    23401 cctgtggaaa tacccccgtc atgaattgac tgtctgcccc cgttaatggt tgtattatta
    23461 gcctgcccca caaaattgtt atgacctcct acatcttgat atccacccga ttcaataaga
    23521 cttccattag atatcccgcc ataaacattc tgctggccat ggttgataat atgagtgtta
    23581 tttgttgtac ctcgttcatc tactttttgg ctgccatcta cagtctcatc gtttaccaca
    23641 ccaataacat caggagtgaa ggccgccatc ccgggcgggg catatatcaa ggcagatatc
    23701 aataaggaaa gtactgagcg acgacaataa tagggactgg tcctgttcat aaatttcatc
    23761 ctctgaaaag tgaatactga gtagcgttta agcgacctta gctttgctgc aacatcagcc
    23821 cacaggcacc agaccagggg attcatcctg aagagacagc gcaagtgtat tgtgttcacc
    23881 gctcatcaaa gacatcatga tgaaatgatg atattccgca taagaatgag gcatttttta
    23941 aacgcagtgc gctgaagtgt ggttggataa aaaagtcaat ctattcagga aacacgggcg
    24001 tattcttttc tttcgacaat caggcagtcg gaaaaataaa atgatttaca taatcgtttc
    24061 tgatgaatat cttctgctca cataaaaatc acacaataac tctgagatcg cagattgttt
    24121 tacttttaca gcttccgtcc ccccattgtt gggcaaatat agattgggcc agagcacgaa
    24181 agttaatacc acgttcgcac agctcctcca acagcacgac aagatgccac atactgcgcc
    24241 ccagtcggtt cagtttacag actagcagtg cgtcccctac cgataatgtc ctgagccgtt
    24301 tgttctgtcc gggcctgctg ccacggttat gcgctgaagt ggtaactctg agcacgcagt
    24361 tcttcagtca ggcgtggtgc accgtcaccc tattattgct gggtaagatc aaaaaacttt
    24421 caagcagcta aggaaagttg aaccagacat tagatgaaat atttcaacca attacagcac
    24481 cagttcagac accgccaact catcaaataa atcaaggggt tacgtgaaag cgtagcccct
    24541 tttactttgg tagtgacagc aaaatggatg cagtgtgaag aataatcccg tttactcaat
    24601 caataataca tattatttca atctacgtta ttatttcttt gtaaaaatcg ccatttattt
    24661 atcattgaaa actgctttta gaactttata caaggggact agtcacaaca ggactattct
    24721 caacgggctc atcctcagag gaactatcat caaagtcatc atccatagat gaaataccat
    24781 cgaatggtgc cacgcccgtg attagtttta ttttattatt acgatcagtc aagactccat
    24841 ttaaaccatt ttcgctgacg gattttaata atttatcata actcttattg tcatcaggca
    24901 cattaaaaat acacggagta tcaacatcaa acaatgacgt tccccagttc acatatttaa
    24961 tatcatagtt actgaagttc tatccagaaa agaagcatcc cttaaaatcc aatccaccta
    25021 aattatataa accaccctct tctttttgga gagcaatgtt aattttggct atctcccgga
    25081 catcatcgcc atttttgtat ttgaataccg tttcaagatg ttctccagac agcttgactt
    25141 ctggaaataa tttgaaatta aaccctacat tattatgtgt caacgtagat gaacaaaaga
    25201 tggaaaaagc ttgcagtgct gaattatagc tatcgatttt atcttgaggc tgcgctcttg
    25261 gtaaaaactt atagcaacac tcataaaaat taagtagaaa ctctgatgac cttccatttt
    25321 tatcaaataa tacccgctga aaatgctgca caaacttatt cgttttttct tgaatatcta
    25381 cggtgtgttc ctttgactct gacaaagatg aagcctgttg aagtgcattg ctcatctggt
    25441 taatgaacat gtgcatttct ttaaatttat ctccatagtg acctttaact tgctgcgagt
    25501 ttttagctac aggtgaaatc ttatctattt ggttgtcata taaattatgt gattccatac
    25561 agtcatttgg cggcgtttct gaaataacac tacgtgttct acctggctga agtgaagcaa
    25621 catgaaaatc actttttatt atattatcga aaagggcctg ttcatttctt ttagcggatt
    25681 gaacatactg atcaatatta ttttctctcg ataatttgtt attttcagaa aacacatttt
    25741 ctttaatctc cggttttaat ttaatgagaa gatcttccca ctcctacttg
//
LOCUS       DAZGQL010000077        25000 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.077, whole genome shotgun sequence.
ACCESSION   DAZGQL010000077 DAZGQL010000000
VERSION     DAZGQL010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25000
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.077"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..755
                     /locus_tag="ACHKIR_003528"
     CDS             <1..755
                     /locus_tag="ACHKIR_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFO9991212.1"
                     /translation="DDIDAILAAADVGCFATYITRQGGVLIPRNTYLVRTAYETAEEA
                     NDYGEFPQRIYGVRAPSLGERYTICTHPDTWKLVRRKPENEDRTDEGFDVECGFSAPW
                     TRGNNCPPDQNLTINDPPERQPDGRREQRLTLPEGDLGEWLRSLTPTERKQLTRQLKS
                     PPPDTETVKNGGISAPAGGVRVSVALTAEPHITAQITRELDALGVQLHESAVVSVRHG
                     ARVRLADGRTSYWNDSTRRIDITPAPNTEAPQ"
     gene            752..1156
                     /locus_tag="ACHKIR_003529"
     CDS             752..1156
                     /locus_tag="ACHKIR_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9991213.1"
                     /translation="MTRFYCAMQRQRFDELMRQRTPLLTLNNIATGPQPGDELEITVV
                     SPDRTTTTIITDILNGRPDSTNRSWLVAVRPRRPPGEPEPLTPAGKIMRQYHEEQAHK
                     HNAELTRTLASINRKAAIARYVHSAAGRQSRR"
     gene            1205..1375
                     /locus_tag="ACHKIR_003530"
     CDS             1205..1375
                     /locus_tag="ACHKIR_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9991214.1"
                     /translation="MNSTRRISDRELTKMIESAQQLLSRQNPVVEETWWENLLAALTE
                     LRERRNNEGAAK"
     gene            1372..1698
                     /locus_tag="ACHKIR_003531"
     CDS             1372..1698
                     /locus_tag="ACHKIR_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFO9991215.1"
                     /translation="MKTPGMTGKEIILRYLETHARFSAPELVEKLGVKRTVINQAAGK
                     MVREGLLVVDGYTNKITHYRKPTEKERQELAQRAERQQEKSVIEACKRSEIMKRILFI
                     YGAAFR"
     gene            complement(1764..2027)
                     /locus_tag="ACHKIR_003532"
     CDS             complement(1764..2027)
                     /locus_tag="ACHKIR_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001217942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HFO9991216.1"
                     /translation="MRIFKARCPDCGAPAVIRKTDWKTPELADLYCACSDVECGHTFV
                     CTMTFSHTLSPGARSGRNMVKFLVDAMRPEDRRYALELLSRRS"
     gene            complement(2065..3117)
                     /locus_tag="ACHKIR_003533"
     CDS             complement(2065..3117)
                     /locus_tag="ACHKIR_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO9991217.1"
                     /translation="MSKKRRNKTSRVTKKAFTPARGSTITFGQPEPVLTTGTDYHNIW
                     YDNEHNHWQLPINRLALAQLPNLNAQHGGVLYARRNMVAGGYTGGGLTTDQMEQAVFD
                     YLLFGDLALLKVRNVFGDVVALEPLPSLYLRRRKTGEFVVLQEGPALVYTPEDIIFLR
                     MYDPRQQIYGLPDYIGGIHSALLNSEASLFRRRYYNNGAHMGFLLYTNDPGITTEMED
                     EIREKVAESKGLGNFRNLYINIPKGSPDGVKIIPIGEVSAKDEFSNIKGISAQDVFTA
                     HRFPAGLAGIIPTNGATIPNPETTRETYRKDEVIPLQRKIMNAVNSDPDIPEHLYLCF
                     DVDAGHVAEEKAVPVG"
     gene            complement(3114..4919)
                     /locus_tag="ACHKIR_003534"
     CDS             complement(3114..4919)
                     /locus_tag="ACHKIR_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HFO9991218.1"
                     /translation="MAKYSDEIKEAARSLYLKRWHPRDIAAELGIPPRTVYHWCDVGQ
                     WATLLPAESVEDVIARRIDVLTGREGKTECELAELRELIAQHVKIIAQKNKHAERMAE
                     IAALRPAGYEDGAAAGTCGGGEGKKRRYRKNDVSGLTAEMLDEWARKNLYAYQLHCRE
                     NRHRERRFILKSRQIGMTWYFAWEAFEDAVVTGDNQIFFSASRVQAEIFREYIINFAL
                     QFGITLTGKHIRLSNGAMLRFLSTNASTAQGFNGHLYGDEVFWIPKFTRLHEVASAMA
                     THDKYRTTYFSTPSAKTHQAYLVWNGDDWRGDDPARRAVEFPKESAMRVGCECPDGIW
                     RYIIRLEEAVAGGLSARVDIERIRNRYNPTTYAMLYGCEFVDSKDAVFKFSELVRCEV
                     EMETWGDYDPTAARPFGNREVWAGFDPSRSGDNSTFVIVAPPVHEGERFRVLAVWQWQ
                     GFNFTWQADQIRELMQRFNITYIGIDITGIGKGVFDIVSRFAPREANAILYSVESKNR
                     LVMKMIDVVAHKRIEWAKDAIDEASRERTEIPASFMSIRRTTTKSGNALTFVAERSDV
                     TGHADVFFAISHAVINEPVDYDYERPSTWAFGKAA"
     gene            5086..6144
                     /locus_tag="ACHKIR_003535"
     CDS             5086..6144
                     /locus_tag="ACHKIR_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HFO9991219.1"
                     /translation="MPQPTLMTDWICIATSGPTVDGREIDPQWLTDAAETYSRNTYTA
                     MLWPWHEEEIGHRQYTSNLGEVDSLKAETGEDGKTRLYARLVPNQYLIEANRLGQKLF
                     TSAEIVENFARSGRDYLLGVAVTDIPASLGTEKIRFTLNNEEYTAQRANCQTFTLGSL
                     SPDTTHAPSKPESFLKRLFSAGQTDNTDNTDTSKPDTGEDAKMDEIKALLEKLITLVE
                     GGSAAAAGEGTADTPEEAKAEVAEIAAEIADAAAEVAELAEEVKAAPEDEVKAEAFTA
                     AKAKLEDAIKAFSTITPEKGERYRRFAARRRDDTDKEEIKAIAEKLNDLTTKLSATGI
                     TPRPAGTPAESEKPFDFV"
     gene            6156..7175
                     /locus_tag="ACHKIR_003536"
     CDS             6156..7175
                     /locus_tag="ACHKIR_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
                     /protein_id="HFO9991220.1"
                     /translation="MQLNQKTESLLRKYAAGLGAANGVDDVTRYFALSDPKETALRDA
                     IMHSDEFLSKLPNVLDVEQVTGQVVTTGVPGLHTGRKKNARFTLPLGVTGNEYKLAET
                     DSGAHLLYTTLVTWANAGSENEFFNRIQNFVNRQIALDILRIAFNGTSVAEDTDPVTN
                     KNGEDVNTGWHAIVKGRSAKQIVEAGPTLGKGGDFIGLDAAVADLVHTLIDESYRGHP
                     DLVVLVAPDLVAQDKVTLLNAIDAPTEKVAAQLIGRQIAGLTAYTPPFMPEGRLIVTT
                     LANLHIYTQRGTRQRRAEWNDDRKRFENNYLRMEGYAVEHDEIYAAYDKLTIKGAEEA
                     EDVGA"
     gene            7193..7891
                     /gene="gpM"
                     /locus_tag="ACHKIR_003537"
     CDS             7193..7891
                     /gene="gpM"
                     /locus_tag="ACHKIR_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit"
                     /protein_id="HFO9991221.1"
                     /translation="MTPCQRHRAKIRTQEALERREALTASPVSFHLLRAELDRDVARL
                     RSLPVREERLAFKRDILLPRWLPVAERYIADGKRHACPVLVYCIIWLFDTGDLSRALD
                     WADIAISEGQATPDNFRSTLPAFVADTVLQWATDNSEAGHSIEPYFSRTFRNVTTTWR
                     LHEEIRAKWFRFAGLYLLRDEAGKPRATAINDTAVLEKADAYLAQAAALSRTAGVKSI
                     RARIRARISALNVA"
     gene            7999..8472
                     /locus_tag="ACHKIR_003538"
     CDS             7999..8472
                     /locus_tag="ACHKIR_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HFO9991222.1"
                     /translation="MSEPSFSVSGKPLKSTPQTVTNGEPFWPDLDLAELQGSRTLPAD
                     LPDDTAAMALLAAVDEVNTTLAPVVAHWKAQGHERAADVPGARMGDETQLVAQYKKAV
                     YARAKADLMGEFATIGRRETYPGQESEDTRATLLAEASLVMRNMLGHRRAGVHII"
     gene            8469..8942
                     /locus_tag="ACHKIR_003539"
     CDS             8469..8942
                     /locus_tag="ACHKIR_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9991223.1"
                     /translation="MNQLESLTAFITENLPPDAMQFFMSSQEDAQLIRSQKALGNDQI
                     RMGVMTYTGVIAWDNFPFRKYSPGLVYALVMAWLEEHADPLREEFRPEDVNVDPQFND
                     EDSCLLDITVGLADPLIIRVQEDGPVPYRGKRWTLVNPEIWTAETMELFVSRSRE"
     gene            8939..9649
                     /locus_tag="ACHKIR_003540"
     CDS             8939..9649
                     /locus_tag="ACHKIR_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000623204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9991224.1"
                     /translation="MISGQLNPQQLAEAQAMLQSLDLPPKKRRRLLWRIARHGIIAAA
                     RRHQRDQTAPDGTAWAPRKRGKGKMFRKLPRLLAVREMPGQDAVKVYFRNGNHTSGRS
                     RISAGAVGWVHHNGAEIRVSADSFSGQDQNGQTATRRQAKRLRELGFKVRQKGRWRKA
                     SMKYLLTLSRAQAGLLITIMEDQIIAGPRQQKARTWTITLPSRVFLGVTNEEFSHILA
                     RQMNAMGYGLDVDAQDIR"
     gene            9661..10806
                     /locus_tag="ACHKIR_003541"
     CDS             9661..10806
                     /locus_tag="ACHKIR_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000118787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2586 domain-containing protein"
                     /protein_id="HFO9991225.1"
                     /translation="MSWPNVTVSHQNRFNGATREVERTLLFVGYGKKNTGNTLSVSPE
                     TDLDDVLGPDESLLKSTLTAAIANGGQNWFAYVHVLSEPKPPAPEGGDANAAWVDAVK
                     KAQTIASAEGVVIAIDITAKDAVNRATETRALLQSAYGRFVWFMLCVAGPGKDEAWAA
                     YVTRISAIQDGVAAPGVMVVPRLWGNEPGVLAGRLCNPSVTVADSPARVATGALVAMG
                     NDEIPQDGKKQPLELATLRSLEGKRFSVPMWYHDFDGLYWSDGRTLDVEGGDYQVIEN
                     VRVVDKVSRRVRLRAIARLADRALNSTPGSIAASQTYFSRTLREMALATEINGVPFPG
                     EVKPPQDGDITIVWTSSTHVQIYIVVRPYESAKAIGVSIELDTALEI"
     gene            10808..11269
                     /locus_tag="ACHKIR_003542"
     CDS             10808..11269
                     /locus_tag="ACHKIR_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000918213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protein"
                     /protein_id="HFO9991226.1"
                     /translation="MAGNERISGGAFDINYDGIMIHVESATVTITDNSAVAQTRGVPN
                     GYTRGSVSADVEVEVDAQNFKKFGAVARSAGSWRAIPEKDFLFYANNGSEEEKIEVFG
                     CVPMLSDVVNISAGEAGKTTKKIKFMVTSPDFISIDGVPYLSARDTRDLKG"
     gene            11274..11579
                     /locus_tag="ACHKIR_003543"
     CDS             11274..11579
                     /locus_tag="ACHKIR_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HFO9991227.1"
                     /translation="MPDGQTSLLAKLIIIGAAIGLGRLMVSRDRITARLLIGRMIMGS
                     AVAPVAAIPLFKFPDMPELVVVGLACALGIMGSAFIEECFCRLADRYLKQKERRNDP"
     gene            11569..12321
                     /locus_tag="ACHKIR_003544"
     CDS             11569..12321
                     /locus_tag="ACHKIR_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000149054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFO9991228.1"
                     /translation="MTHKPYISPSLSLYHAQAHDVLRMLPENSVDALITDPPYSSGAT
                     HKAGRTCQGSHAKYINDEDLHRFEGFEGENMDARSWEYWTHLWMTQAWRTVRPGGYAL
                     VFTDWRQLPALTDAFQAAGFTWRGIVAWNKGRASRTPHTGYFRHQCEYVVWGSKGALG
                     KSPSGPFDGCITHHTRPQEKQHPTGKPESLMDELVRVCEPGGLILDPFMGSGTTGVAA
                     LRRGCRFTGIELNAHYFSVAQARLTGTEQRND"
     gene            12321..12659
                     /locus_tag="ACHKIR_003545"
     CDS             12321..12659
                     /locus_tag="ACHKIR_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007710822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HFO9991229.1"
                     /translation="MKLSEKQQLFAVMIADLIHWAQEHGYRLTFGEAYRTPEQAALNA
                     KSGKGIRNSLHTLRLAVDFNLFINGKYQADTDAYRPLGEYWESIGGTWGGRFSRADGN
                     HFSLEHNGVK"
     gene            12656..13042
                     /locus_tag="ACHKIR_003546"
     CDS             12656..13042
                     /locus_tag="ACHKIR_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9991230.1"
                     /translation="MMKQLAGCLVILFAAMWAGWLAHDWHDARVALAEQEAMEATRRA
                     VTEAGQQSARILEKRLEALKGNEIHTERVIHTETVRPVFRNVCATDDYVRLFNARAEA
                     AERTLAGKPADGMPEDTAETGRPDGR"
     gene            13142..13417
                     /locus_tag="ACHKIR_003547"
     CDS             13142..13417
                     /locus_tag="ACHKIR_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative phage tail assembly chaperone"
                     /protein_id="HFO9991231.1"
                     /translation="MTTKNTGTIILSVAGVTLSFEPNKTAFNNLINEITMTNKVAPMS
                     TYLNRIVTPASRDALADLIARYPGCEMQIVEKINEVYSPKLEIELKN"
     gene            complement(13381..13608)
                     /locus_tag="ACHKIR_003548"
     CDS             complement(13381..13608)
                     /locus_tag="ACHKIR_003548"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9991232.1"
                     /translation="MSPSVKCDRYTFSGLVGAGTGKIFIQPDGTGDALFVVRLSGEVV
                     TPQCQILLKGIAADAFDPRGQFFNSISSFGE"
     gene            13607..15793
                     /locus_tag="ACHKIR_003549"
     CDS             13607..15793
                     /locus_tag="ACHKIR_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO9991233.1"
                     /translation="MASQLDFIINLTDQLTRPLRQVQESVTGFAERSQGAFARIGVGV
                     AGLWGVAEGIRAAMDPAWEMQKALGELEAKGVGAQALTALTTQAMKLGTAFGQSSTEI
                     IRSAWHIRGALDGLSDGDLPRVTAATSTLAVAARVSSEEASGYLAAMGARFRSEFSAM
                     GAVGFAEDLAGKTAWMVQQFGVEMSTMQELIEGTKNAGADFGVSMAEQFAILGTLSRT
                     MGTEASGIYEEFLRSAPAAAQKMGMSFVDATGKILPMGDILDRLQARFGRNIEGNVAA
                     QQALDDAFGSGADVIKKLWGQQEMLNRSISALGRNDGMKRATEMAEAMTDPWDRLVAS
                     FYNIRAAIGNALYPILAPLAGRFADIGATFVRWLNMFPNIARWLGYITLTMLALGAAG
                     AVTNIVLGVFSFIMEGHKAIIKGARAAWSLYTLALTKVRSVIIATSLAARLAGVSFLT
                     MGAPVLLVVAAIAALVVVAIKFWQPVKAFIRGFIDGFTGALDAMSPVTAAFGDIARAL
                     APVWQGIKTLFGAFSDLFAPVQLTTGQMNSVTRAGETCGRVVAAAVSLISPALGVVIS
                     VIRNLIDIFTIVIRGWQDVVAAFDPTAPVESFRKMAGIIGNVFGDLWNVIKNSFNRTW
                     NWLAEKLNKIPGISIDMKPTEQVNTMTRGATGSTVPPQIAETTVPALAGNRISAELPP
                     GGIRNQIRTDTSTRIDNSRTFGNLTINTQNPMTPGQLDEWTELYAG"
     gene            15790..16140
                     /locus_tag="ACHKIR_003550"
     CDS             15790..16140
                     /locus_tag="ACHKIR_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2590 family protein"
                     /protein_id="HFO9991234.1"
                     /translation="MNTPSLYIDLLITDGSFTLNSGNEPQRCDNRVSIGQDVVHSIIE
                     SGIAALLIGERSPTLRSDIITRLILLVEDDERLVPGTVFINEEAPGRLHVTAETQDFG
                     RINTRVNYERETGR"
     gene            16118..17302
                     /locus_tag="ACHKIR_003551"
     CDS             16118..17302
                     /locus_tag="ACHKIR_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016507090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFO9991235.1"
                     /translation="MKGKPDVDFNQVFRESGMPETEEEIATLFHEDVQKTGLITNTSP
                     MSPFWRLILTIVIRPVQWLRDALVDVVLPGMFVATADRSLLLLHAHDVNITPKPASKA
                     SGVIWFAKTRANDDVLIPEGTIVQTARINGVIYSMAVTEETLISSGKSGASVPVEATT
                     EGSGHNLAPGYFRILPVAIPGIASVVNREDWLTVPGADEESDDELRDRIRNQFNLVSS
                     YHTDAVYRGLIASVAGLSIDRIYFQHDAPRGPGTANAYLLLDSGEASAPFIEAVNRYI
                     NEEGHHGHGDDLQCFAMPETRHRLDVTAYIRSSTTLPPDGAEGLKQDIGDLIRCAFRE
                     NARFNVKKVWPYSRFSFSTLGKEIHQTFPVVASVQFSLEDIISDLAVPRLESLTVEVV
                     IE"
     gene            17295..17843
                     /locus_tag="ACHKIR_003552"
     CDS             17295..17843
                     /locus_tag="ACHKIR_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9991236.1"
                     /translation="MNDFLQRLRSLKLPSWMDRGEPDKLLRAARAFWSRVWEWITWPV
                     RQFDPMTCAEPVLDLLAWERDITRFKGEPTEIYRRRISYAFINAQEAGEVAGFIAIFE
                     RLGIGYVELLERQPGVDWDVILVRVTDSQIADNSDLLLEIIRKYGRTCRRYRFEVETL
                     TPFIIHYGAYEGEYICWTARAE"
     gene            17866..19932
                     /locus_tag="ACHKIR_003553"
     CDS             17866..19932
                     /locus_tag="ACHKIR_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9991237.1"
                     /translation="MAKSVITSAFIRLKAQQAAEGTPVVLDEFIFANVPDLNPETPVS
                     PDETLPPEDQIVHRQAVGKTGVVNENAVVYSVTLGAELGDFEFNWVGLVSKATSTLAM
                     IVHAPTQKKIRTHNGNEGNVLVRSMLMEYSGAKEATGITTPAQTWQIDFTARLAAMDE
                     RQRRENVDIYGKATFFDDGWLVKKGETWTVNKGVGYVEGLRAELTEDTPMVTPTSPSR
                     IWLNVWWTGTLTSEWTVEHEIIPADTDVTEERLRKTYPRDGIQQYFYAIAEIDEDGNV
                     TDLRPRGTPEERRSLDALKKHEKSRNHPDATLKEKGFTQLSSATTSTSETQAATPKAI
                     KIAMDNADGRLAKNRNGEDIPNKELFLINVGAPRVYAKDGYVGEAGQRWTTEQFVEWL
                     NERGAFRPGLWIFKANISLSRHSVLTDTGYGDICLGGCLIEVSGDINGGIIRITTGLE
                     SPNPADMYLRTQFTGVVLNKGKSTIWYRTYTDKNKPTPQDIGALPASELLPAGVPLPW
                     PSDTPPAGYAIMVGQTFDKARYPLLAKAYPDGRIPDMRGWTIKGKPASGRAVLSQEQD
                     GIKSHTHGGSVSNTDLGTKTTSSFDYGTKTTSTNGQHEHSVPLRRQDGGGINFDWLDG
                     SSSGSHIGNGYLPAAGDHQHTVPIGAHTHTLALGSHGHGLTINPTGNAENTVKNIAFN
                     YIVRLA"
     gene            19932..20528
                     /locus_tag="ACHKIR_003554"
     CDS             19932..20528
                     /locus_tag="ACHKIR_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO9991238.1"
                     /translation="MTFKMSEHPQTIKIYNLSAGTNEFIGEGDAWIPPHTGLPAHSTD
                     IAPPDIPEGHVAIFDEDENTWQLMEDHRGKTVWRLESGSALLITESGPLPENVTIIKP
                     DGEFCRWDGSSWAKDEAAEQAYRVSEAENKKRELIQEATNTINILQDAVDLNMATDDE
                     SALLPLWKKYRVLLSRTDTGNAPDIVWPESPSPDHHAG"
     gene            20540..21265
                     /locus_tag="ACHKIR_003555"
     CDS             20540..21265
                     /locus_tag="ACHKIR_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9991239.1"
                     /translation="MTWTRKTISVSADMPPLSCSVIPVTPWTYGLGRYEESGVYLSPP
                     NAINWLAGKMAGSRASGDVTIIMIAENTHDLFIQSMAALTAVLPVPVFTQAQRMAQAA
                     ASLSTDKMQIPITTDTLPAPVHLSVATMRSAVSAARAAQARQLAAQRPDAAGLRRQIS
                     RFISRRAGALAGLEEGIRTLAEQKAQAWVFQYRGYHRAAAAAMVKDIPAPTAVHTVAA
                     LLAADSLTELGKMIHEPDRTARP"
     gene            21237..21779
                     /locus_tag="ACHKIR_003556"
     CDS             21237..21779
                     /locus_tag="ACHKIR_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9991240.1"
                     /translation="MSQTALLALDGEGIFMQNMVISPSMQFQEKDQSGQTSSTANAEQ
                     GIKAKELRVTGMITFDNERALQRIFQLASATTGDGALKVYRIANATAAAINFREGTFS
                     GQIDAQQQTDRLAWQVSFTLRERRSVPEKRQARATPASSRKQTPQTPGAKGGTVADET
                     PEKMTWFEEKVLKPVNDALE"
     gene            21782..23455
                     /locus_tag="ACHKIR_003557"
     CDS             21782..23455
                     /locus_tag="ACHKIR_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9991241.1"
                     /translation="MNPTKRLLLGNDTIQLVSCSVMLELNACGRGFITARTEQNYTGR
                     PVRLDIGYGNDLVRWFTGYVERSQPAENGSVRLLIREMAGILDHPFPCSFQHPTMRTV
                     TQWLSETSGLEIILPDNAAYTDRPVPHFTHSGTGYELLASLGRIFSVPDYIWHPLPDG
                     GIMTAAAAQGMFSGRPVTIPHEFSQASTGGHSMTLPMIQTLRPGVEVNGQRLTMVMLE
                     DDNMTITWTPRNKATGAPLQKSPFRRQAEKAFPELASGLHLPKLARVEAPTEAVSAGN
                     IADPFRPRYAVNLQLLDENGNPAADTPVYNAVPLPVPMAGSESGMFQFPPPGTLVEVG
                     FVEGRADKPFIRQIMAEGHNLPDVKPGEQLQQQRDGVSQRVTVAGDWERQTDQRIQET
                     SRERVVSADEEKRSLTSRETTIQATDTTTVLGVSTLQAGAVTHITEENYSISTGGHMM
                     VVAGTCERDVEGDTQDTVGGSLTERIKGVRHSIALAQKLVGDTVKLGNEGINVLTMLT
                     DLADVVEELADITASHTHPKTGTSPQAAQFSQVAQECRQLKNKYSPIIE"
     gene            23873..24208
                     /locus_tag="ACHKIR_003558"
     CDS             23873..24208
                     /locus_tag="ACHKIR_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9991242.1"
                     /translation="MQGFAKVCKNSGFLRIPKNFNALNNKRFLKLRHDRRSEKGSGDE
                     NGKIRKTRTGAAWRGVAAKITFAHFCTTAPKKDGEGEGKQPDHPLHVTDVKGKLRAGA
                     FRLTAGRNT"
     gene            24318..24458
                     /locus_tag="ACHKIR_003559"
     CDS             24318..24458
                     /locus_tag="ACHKIR_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO9991243.1"
                     /translation="MPQKTIIVSILCITILMIVWMIHKSPCEFRLNIMWSQFAAFLQC
                     KQ"
     gene            24776..24916
                     /locus_tag="ACHKIR_003560"
     CDS             24776..24916
                     /locus_tag="ACHKIR_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO9991244.1"
                     /translation="MPQKTIIVSILCLTMLLTVWMIHRSPCEFRLNIMWSEFAAFLQC
                     KP"
BASE COUNT         5970 a         7296 c         7107 g         4627 t
ORIGIN      
        1 atgacgacat tgacgccata ctggccgccg ccgacgtggg atgttttgcc acctatatca
       61 cccgccaggg tggtgtgctc attccccgca acacgtacct ggtccgcacc gcatacgaaa
      121 ctgccgaaga ggccaacgac tacggcgaat tcccgcaacg catttacggc gtccgcgccc
      181 cgtcactcgg ggagcgctac accatatgca cccacccgga cacctggaaa ctggtcagac
      241 gtaagccgga aaacgaagac cgcacggacg agggttttga cgttgagtgc ggcttttccg
      301 caccttggac tcgtggcaat aactgtcccc ctgaccagaa tctgaccata aacgacccgc
      361 cagaaaggca gccggacgga cgcagggagc agcgacttac cctgccggaa ggtgatttgg
      421 gtgaatggtt aaggtcactg acccccacag agcgaaaaca actcaccagg cagctgaaaa
      481 gcccgccacc tgacacggaa accgtgaaaa acggcggcat cagtgcgccc gctggcggcg
      541 ttcgcgtgag tgtcgctctg accgctgagc cacacatcac cgcacaaatc acccgcgagc
      601 ttgatgcgct gggcgtgcag ctgcatgaaa gcgccgtcgt ttccgtccgt cacggtgccc
      661 gcgtgcgcct ggctgacggg cgcacctcgt actggaatga cagcacccgc cgcattgata
      721 tcacccccgc accgaatacg gaggcgccac aatgacccgc ttttattgcg ccatgcagcg
      781 acagcgtttt gacgaactga tgcgccagcg cacgccctta ctgaccctga acaacattgc
      841 cacggggcca caaccgggcg atgagctgga aatcacggtc gtcagtcccg atcgcaccac
      901 cacgaccatc atcaccgaca tactcaacgg acgaccggac agcaccaacc gcagctggct
      961 tgtcgccgtt cgtccgcgcc gtccgcccgg agagccggag ccgctcacgc ctgccggaaa
     1021 aatcatgcgg cagtaccacg aagagcaggc acacaaacac aacgcagaac tcaccagaac
     1081 actggcatcg attaaccgca aagcggcaat tgcccgctat gtgcattcag ccgctggcag
     1141 acaatcacgc aggtaaacaa agaccatgaa cataaccaga aacagccaac taaaggagcg
     1201 caccatgaac agcaccagga ggatcagcga cagagagctt acgaaaatga ttgagtcggc
     1261 acagcagctg ctttcacgac aaaacccggt cgtggaagaa acatggtggg agaatttact
     1321 ggcagcactg accgaactcc gggaacgccg gaacaacgaa ggagccgcca aatgaagacc
     1381 ccaggaatga ccggaaagga aatcatttta cgctacctgg aaacgcacgc acgtttcagc
     1441 gccccagaac tggtagaaaa actgggcgtg aaaagaaccg tcattaatca ggccgccgga
     1501 aaaatggtac gggaagggtt gctggttgtg gacgggtaca cgaacaagat aacgcattac
     1561 cggaagccaa cggaaaagga acgacaggaa ctggcgcaac gggctgaacg gcagcaggaa
     1621 aaatcagtca tcgaagcctg caagcgcagc gaaatcatga agcgaattct gtttatctac
     1681 ggcgctgcat tccgttaaag gagctggtga gtcggggcaa aaattctttt tgccccggcg
     1741 aagcagcaga caagcgaagc gcgtcaggaa cgccgcgaca gtagttcaag cgcataccgg
     1801 cgatcttccg ggcgcatggc atccaccagg aacttcacca tattgcgacc ggagcgggca
     1861 ccggggctga gggtatggga aaaggtcatc gtacagacga aggtgtgccc gcattcgaca
     1921 tcactgcatg cacagtacag atccgcaagt tcaggggttt tccagtctgt cttccggatg
     1981 acagcaggtg cgccacagtc aggacaccgg gctttaaaaa tccgcatctt acctcccgat
     2041 aacacgtatt ccggggagga cattttaccc gaccggcacc gctttttctt ccgccacatg
     2101 cccggcatcc acatcaaaac aaagataaag gtgttccggt atatcgggat cgctgttcac
     2161 ggcgttcatg attttacgct gaagcggtat cacctcatcc ttgcggtacg tttcacgcgt
     2221 cgtttccggg ttagggatgg tggccccgtt cgtggggata atccccgcaa gaccggcagg
     2281 aaagcggtgt gccgtaaaca catcctgggc gctgatcccc ttgatgtttg aaaattcatc
     2341 cttggcgctc acctcaccaa tcggaataat tttcacccca tccggcgaac ctttggggat
     2401 gttgatgtac agattgcgga aatttcccag ccccttagac tccgccacct tctcgcggat
     2461 ctcatcctcc atctccgtgg tgatcccagg gtcgttcgta tacagcaaaa agcccatatg
     2521 cgccccgttg ttgtagtaac ggcgacgaaa aaggctggcc tcgctgttca gcagcgccga
     2581 gtggatgcca ccgatataat ccggcagccc gtaaatttgc tgacgcggat catacatccg
     2641 cagaaagata atatcttccg gcgtatagac cagcgcggga ccttcctgaa gcaccacgaa
     2701 ctcaccggtt ttgcggcgcc ggagatacag cgacggaagc ggctcaagtg ccacgacgtc
     2761 gccgaatacg ttacgcacct tgagcagggc gagatcgccg aacaacaggt agtcaaatac
     2821 cgcctgttcc atctggtccg ttgtgagacc accaccggta tagccccccg ccaccatgtt
     2881 acggcgggca tacagcaccc caccgtgctg ggcgttaagg ttcgggagct gcgccagtgc
     2941 caggcggtta atcggcagct gccagtggtt gtgttcgttg tcataccaga tgttatggta
     3001 gtccgtgccg gtggtcagca ccggttccgg ctgaccaaac gtgattgtgc tgccacgtgc
     3061 gggcgtgaat gcttttttcg tcacgcgtga cgttttgttt ctgcgtttct tgctcatgcg
     3121 gctttcccga aggcccatgt ggacgggcgt tcatagtcat aatctacagg ctcattaatc
     3181 acggcgtgcg aaatggcaaa aaacacatcc gcatgccccg taacatccga ccgttccgcc
     3241 acgaacgtca gcgcgttacc gctttttgtg gtggtgcggc gtatggacat gaacgacgcg
     3301 gggatctccg tgcgttcacg gctggcctca tcaatggcgt ctttcgccca ctcgatacgc
     3361 ttatgggcca ccacgtctat cattttcatc accaggcggt ttttgctctc cacgctgtaa
     3421 agaatggcgt tagcctcacg tggcgcaaag cgactgacga tatcaaacac ccctttcccg
     3481 ataccggtga tatctatgcc gatataggta atgttaaagc gctgcatcag ttcgcggatc
     3541 tgatcggcct gccaggtgaa gttaaatccc tgccactgcc agacggcgag cacacggaac
     3601 cgctcgccct catggacagg cggagccaca attacaaacg tggaattatc cccggacctt
     3661 gacgggtcaa agcccgccca cacctcacga ttgccgaacg gacgtgccgc cgtgggatcg
     3721 tagtcacccc acgtctccat ctccacctca cacctgacca gttcggagaa cttaaatacc
     3781 gcatccttgc tgtcaacaaa ctcgcagccg tacagcatcg cataggtggt cgggttgtag
     3841 cggttcctga tacgttcaat atcaacccgt gcagacaggc cacccgccac cgcctcttcc
     3901 agccggataa tgtagcgcca gatcccgtca gggcattcac agcccacacg catcgcgctt
     3961 tctttcggaa actcaacagc acgacgcgcc ggatcatccc cgcgccagtc gtcaccattc
     4021 cagaccagat aagcctggtg ggttttggca ctgggtgtgg aaaaatacgt cgtgcggtat
     4081 ttgtcgtggg tcgccattgc cgaggccact tcatgcagtc gcgtgaattt tgggatccag
     4141 aacacctcat cgccgtaaag atggccgtta aatccctggg cggtactggc attggtggac
     4201 aggaaacgca gcattgcacc attgctcaga cggatatgct tacccgtgag cgtgatcccg
     4261 aactgcaggg cgaagttaat aatatattca cggaagattt ccgcctgtac ccgtgacgca
     4321 gaaaagaaaa tctggttatc acctgtcacc accgcatctt cgaacgcctc ccatgcaaaa
     4381 taccaggtca tgccgatctg gcggctcttg aggatgaaac ggcgctcacg gtgccggttc
     4441 tcgcggcagt gcagctggta ggcataaaga tttttacgcg cccattcatc cagcatttca
     4501 gcagtcagtc cggaaacgtc gtttttccgg tagcgccgct ttttcccttc tcccccgccg
     4561 caggtccccg cagccgcgcc gtcctcatac cctgccggac gcagggcggc aatttccgcc
     4621 atcctttcag cgtgtttgtt tttctgggcg atgatcttca cgtgctgtgc gataagttca
     4681 cgcagttcgg caagttcaca ctctgtcttg ccctcccgtc cggtcagcac atcaatacgc
     4741 cgggcgatca cgtcctcaac ggattcagcg ggcagcaggg tagcccactg ccccacatca
     4801 caccagtggt aaaccgtgcg cggcggaata ccgagttccg ccgcaatatc gcgggggtgc
     4861 caccgcttca ggtaaagcga tcgcgccgcc tctttgattt cgtcagaata tttagccatg
     4921 cgcccattat ggcggcgcat ccggcccgca ttcaggcaga aaatttcggc tgtgttcggc
     4981 tatcccacgt tatccgaaga cagccgaatt cagccagtag ccccccgctg taaaaatccc
     5041 gacactcacc ggagttcttc agattttcac gtaaaagagg ccacgatgcc gcagccaacg
     5101 ttgatgaccg actggatatg tatcgccaca tcaggaccga ccgttgacgg ccgcgaaatt
     5161 gatccgcagt ggctgactga tgccgctgaa acctattccc gcaacaccta cacggcgatg
     5221 ctgtggccct ggcatgagga agaaatcgga caccgccagt acaccagtaa tctgggtgaa
     5281 gtggactcac tgaaagcgga aaccggcgag gacggtaaaa cccgcctcta cgcgcggctg
     5341 gtccctaacc agtacctgat tgaagccaac agactggggc agaagctgtt cacatccgcc
     5401 gagatagtgg agaacttcgc ccggagcgga cgcgactatc tcctgggcgt ggcggtgacg
     5461 gatatccccg ccagtctggg gacggaaaaa atacgcttca ccctgaacaa cgaggaatac
     5521 acagcacagc gggcgaactg ccagacgttc acgctgggca gcctgtcacc ggacaccacc
     5581 catgccccca gtaaacctga atcattcctg aaacgcctgt tcagcgcagg acagaccgac
     5641 aacaccgaca acaccgatac cagtaagcct gatacaggcg aggacgcaaa aatggacgaa
     5701 attaaagcac tgcttgaaaa gctgatcacc ctggtggaag gcggcagcgc cgctgccgct
     5761 ggcgagggca cagcagacac gccggaagaa gcaaaagccg aagtcgcgga aatcgccgcc
     5821 gaaatcgccg acgccgccgc agaggtggca gagctggcag aagaagtgaa agccgccccg
     5881 gaagacgagg ttaaggccga agcctttacc gctgcaaaag cgaagctgga agacgccatc
     5941 aaagccttca gcaccatcac accggaaaaa ggcgaacgtt accgccgctt tgccgcccgt
     6001 cgccgggacg acaccgacaa agaagaaatc aaagccattg ccgaaaaact taacgacctg
     6061 accacgaagc tgtcagccac cggcatcacg ccacgcccgg ctggcacacc ggcagaaagc
     6121 gaaaaaccgt ttgactttgt ttaaggacac gcaacatgca actgaaccag aaaaccgaat
     6181 cactgcttcg taagtacgct gccgggctgg gcgcggcaaa cggggttgac gacgtcacac
     6241 gctattttgc gctgtctgac ccgaaggaga ccgcgctgcg tgacgccatc atgcactccg
     6301 acgagttttt atcaaaactg ccgaacgtgc tggatgttga acaggtgaca ggccaggtgg
     6361 ttaccaccgg cgttccgggc ctccataccg gacgtaaaaa aaatgcccgc ttcacccttc
     6421 cgctgggtgt taccgggaat gaatacaagc tggctgaaac cgattccggc gcccacctcc
     6481 tgtacaccac actggtcacc tgggcgaacg caggaagtga aaacgagttc tttaaccgca
     6541 ttcagaattt cgttaacaga cagattgcgc tggatatcct gcgcatcgcc tttaacggta
     6601 ccagcgtagc cgaagatacc gacccggtaa ccaacaaaaa cggcgaggac gtcaacaccg
     6661 gctggcatgc catcgtcaaa ggccgcagcg caaaacagat cgtggaggct ggcccgacgc
     6721 tgggcaaggg tggcgacttt atcgggctgg atgcagccgt ggcagacctc gtgcataccc
     6781 tcattgatga gtcgtatcgc ggtcatccgg atctggtggt actggttgcg cccgatcttg
     6841 tggcacagga caaggtgacg ctgcttaacg ccatcgacgc ccccacggaa aaagtggccg
     6901 cgcagcttat cggtcgccag atcgccgggc tgacagcata caccccgccg ttcatgccgg
     6961 aagggcgcct gattgtcacc acgctggcaa accttcacat ctacacgcag cgcggcaccc
     7021 gtcagcgccg tgcggagtgg aacgacgatc gcaagcgttt tgaaaataac tatctgcgca
     7081 tggaaggtta cgccgtggag cacgacgaaa tttacgcggc ctacgacaag ctgaccatca
     7141 agggcgcaga agaagccgaa gacgtcggcg cataagcaca gagggcggcg caatgacacc
     7201 ctgccagcga caccgggcga aaatccgcac acaggaggca ctcgaacgcc gggaggcgct
     7261 gacggcctcc ccggtcagct tccacctgtt acgcgcggag cttgatcgcg atgtggcacg
     7321 gctgcgcagt cttccggtcc gtgaagagcg gctggcgttc aagcgcgaca ttctcctgcc
     7381 gcgctggctg cccgtcgcgg agcgctatat cgctgacgga aagcgccacg cctgcccggt
     7441 actggtgtac tgcatcatct ggctgttcga cacgggcgac ctgagtcgcg cccttgactg
     7501 ggcagacatt gccatcagtg aaggccaggc cacgccggac aacttccgta gcaccctgcc
     7561 cgccttcgtg gccgacacgg tgctgcagtg ggccaccgac aacagcgagg ccggacacag
     7621 catagagccg tatttttcgc gcaccttcag gaacgtcacc accacgtggc ggctgcatga
     7681 ggaaatccgc gcgaagtggt tcagatttgc cgggctgtat cttctgcgcg acgaggcggg
     7741 caaaccgaga gccaccgcga ttaatgatac tgccgtactg gaaaaggcag acgcatacct
     7801 ggcacaggcg gcagcactga gccgtaccgc tggcgtaaaa agtatcaggg cgcgcatcag
     7861 ggcacgcatc agcgccctta atgtagcgta aacgactacc cgcagggccg ggcgggcgcg
     7921 ggaggaggca tcagtgacgg aaactgtatg gccgtggaat cccggacagc ccgccttttt
     7981 attcatatga ggcacgtcat gagcgaaccg agcttcagcg tcagcggtaa accgctgaaa
     8041 tccacaccgc agacggtgac aaacggcgag ccgttctggc cggaccttga tctggctgag
     8101 cttcaggggt cacgcacgct tccggctgat ttacccgacg acacggcggc catggcgctg
     8161 cttgccgcag tggacgaggt gaacaccacg ctggcgccgg ttgtggcgca ctggaaggcg
     8221 caggggcatg aacgcgccgc agacgtaccg ggggccagga tgggcgatga aacccagctg
     8281 gtcgcacagt acaaaaaggc cgtttatgcc cgtgccaagg ccgatcttat gggcgagttc
     8341 gccaccattg gtcgccgtga aacctatccg gggcaggaaa gcgaggacac acgcgccacc
     8401 ttactggctg aagcctcgct ggtcatgcgt aacatgctgg ggcacagacg cgcaggagtg
     8461 cacatcatat gaaccagctg gaaagcctga ccgccttcat taccgaaaat ctgccgcctg
     8521 acgccatgca gttcttcatg agcagtcagg aagacgcgca gcttatccgc agtcagaagg
     8581 cgctgggtaa cgaccagatc cgcatgggcg tgatgacgta caccggcgtg attgcatggg
     8641 ataacttccc gttccgtaaa tactcaccgg ggctggttta cgcgctggtc atggcctggc
     8701 tggaagagca cgcggaccca ctgagggagg aattccgccc ggaggatgtg aacgtcgacc
     8761 cgcagtttaa cgatgaggac tcgtgccttc tcgatatcac ggtgggactg gctgacccgc
     8821 tgatcatccg ggtgcaggaa gacggccccg ttccgtacag ggggaaacgc tggacactgg
     8881 ttaaccctga aatctggacg gcggaaacca tggagctgtt tgtcagcagg agccgcgaat
     8941 gataagcggc cagctgaacc cgcagcagct ggcagaagcg caggcgatgc ttcagtcgct
     9001 ggatctgcca ccaaaaaaac gccgtcgcct gttatggcgt attgcccggc acggcattat
     9061 cgccgcagcc agacgccatc agcgcgatca gaccgcgccg gacggcacag catgggcacc
     9121 ccggaaacgc ggcaagggga aaatgttcag aaagctgccg cgtcttctgg cggtccgtga
     9181 gatgcccgga caggatgccg taaaggttta tttccgtaac ggcaatcaca ccagtggcag
     9241 gagccgcatc agtgccggtg ccgtgggctg ggttcaccac aacggcgcag aaatccgggt
     9301 cagtgctgac agcttcagcg gtcaggacca gaacgggcag accgccaccc gccgacaggc
     9361 aaaacgactg cgggagctgg gctttaaggt acgccagaag gggcgctggc ggaaagcatc
     9421 catgaaatac ctgttaacgc tttcgcgggc gcaggccggg ttgctgatca ccattatgga
     9481 agatcagatt atcgccggac cacgccagca gaaggcccgg acatggacca ttacccttcc
     9541 gtcgcgcgtc tttctggggg taacaaacga agaattttca cacatcctgg cacgccagat
     9601 gaacgcaatg ggctacggcc tggacgttga cgcacaggat atcagataag gagtcacacc
     9661 gtgagctggc cgaatgtaac cgtaagccat cagaaccgtt tcaacggtgc cacccgtgaa
     9721 gtggagcgca cgctgctttt tgtgggctat ggcaaaaaga acacaggtaa caccctttcc
     9781 gtctcccctg agaccgacct ggacgacgtg ctgggaccgg atgaaagcct gcttaaatcc
     9841 acgcttaccg ccgcaatcgc caacggtggc cagaactggt ttgcctatgt tcacgttctg
     9901 tctgaaccca aacccccggc accggagggc ggcgacgcta acgccgcatg ggtggatgcg
     9961 gtgaaaaaag cgcagaccat cgcctctgcc gaaggcgtgg tgattgccat tgacatcacg
    10021 gccaaagatg ccgttaaccg cgccaccgaa acccgcgccc tgcttcagtc cgcttatggc
    10081 cgttttgtct ggttcatgct gtgcgtggcc ggaccgggca aggatgaggc atgggcggca
    10141 tacgtcaccc gcatcagtgc catacaggac ggcgtggcag cacctggcgt gatggtggtt
    10201 ccccgcttat ggggaaatga gcccggcgta ctggcgggcc gtctctgtaa tccttccgtc
    10261 accgttgcgg acagtccggc acgtgtggca accggtgccc tggtggcgat gggtaacgat
    10321 gagatcccgc aggacggcaa gaaacagccc cttgagctgg ccacgctgcg ctcgctggaa
    10381 ggcaaacgct tcagcgtgcc catgtggtat cacgatttcg acgggctcta ctggtcagac
    10441 ggacgcaccc ttgatgtgga gggtggcgat taccaggtca tcgaaaacgt gcgcgtggtg
    10501 gataaggtct cccgccgtgt acgtctgcgc gccattgccc gccttgcaga tcgcgcgctc
    10561 aacagcacac cgggcagcat tgccgccagc cagacatatt tcagccgcac gctgcgtgaa
    10621 atggcgctcg ccacggagat taacggcgta ccgttccccg gggaggttaa accgccacag
    10681 gacggggata tcaccatcgt ctggaccagc agtacacacg tgcagattta cattgtggta
    10741 cgcccctacg agagcgcaaa agccattggc gtaagtattg aactcgacac agcactggag
    10801 atctgacatg gccggaaatg aacgtatcag cggcggcgcg tttgatatca actacgacgg
    10861 gatcatgatc cacgtggaaa gcgccacagt caccatcacc gacaacagcg ccgtggcaca
    10921 gacacgcggc gtgccgaacg gctacacccg tggttccgtt tctgccgatg tggaggtgga
    10981 agtcgacgcg cagaacttta aaaagtttgg tgctgtggcc cgctctgcgg ggtcatggcg
    11041 tgccattcct gaaaaggatt ttctctttta tgccaacaac ggcagcgagg aggaaaaaat
    11101 cgaggtgttc ggctgcgtcc ccatgttgtc ggacgtagtg aacatcagcg caggtgaagc
    11161 cggaaagacc acgaaaaaaa tcaagttcat ggtcacaagc ccggacttta tcagcattga
    11221 cggcgtgccc tacctgtcag cccgtgacac ccgcgatctg aaggggtgat gccatgccgg
    11281 acggtcagac ttcactcctg gcaaaactga ttatcatcgg tgctgccatc gggctggggc
    11341 gtctcatggt gagccgcgat cgcatcacag cccgtctgct tatcgggcga atgattatgg
    11401 gctctgccgt ggcaccggtt gccgccattc cgctgtttaa atttccggac atgccggagc
    11461 tggtagtggt cggtctggcg tgcgcgctgg gcattatggg cagcgccttt attgaagaat
    11521 gcttttgccg tctggcagac cgctacctga agcagaagga acgcagaaat gacccataag
    11581 ccttacattt cgccttccct gagcctgtat cacgcgcagg cgcatgacgt tctgcggatg
    11641 ctgccggaaa acagtgtgga cgcgctcatc acagacccgc catacagttc aggtgccaca
    11701 cacaaggcgg ggcggacatg ccagggcagc cacgccaaat acatcaacga tgaagatctt
    11761 caccgctttg aaggcttcga aggcgagaac atggacgccc gttcatggga atactggacg
    11821 catctgtgga tgacacaggc atggcgcacg gtacgacccg gtggttacgc cctggtcttc
    11881 accgactggc ggcagttgcc cgcgctgacg gacgcattcc aggccgccgg ctttacctgg
    11941 cgggggatcg tggcatggaa caagggacgg gcctcaagaa caccgcatac cggttatttt
    12001 cgtcaccagt gcgaatatgt ggtctggggc agtaaaggcg cgctgggcaa atccccttcc
    12061 gggccgttcg acggctgcat cacgcaccac acacgcccac aggagaaaca gcacccgaca
    12121 ggtaaaccgg aatcgttgat ggatgagctt gtgcgcgtgt gtgaacccgg agggctgatc
    12181 ctcgatccgt ttatggggtc cggcaccacg ggcgtggccg cccttcgccg gggctgccgt
    12241 tttacgggta ttgaactgaa cgcccactat ttcagcgtgg cacaggcgcg ccttacgggg
    12301 acggaacaga ggaatgactg atgaaactga gcgaaaaaca acaactgttt gccgtcatga
    12361 ttgccgatct catccactgg gcacaggagc atggctatcg cctgaccttc ggcgaggcat
    12421 accgcacgcc ggagcaggcc gcactgaatg cgaaaagcgg aaaaggcatt cgcaacagcc
    12481 ttcacacgct gcgcctcgcg gtggacttca acctgttcat taacggcaaa taccaggccg
    12541 ataccgacgc ctacaggccg ctgggggaat actgggaatc catcggcggc acatggggcg
    12601 ggcgcttcag ccgggcagac ggcaatcatt tcagccttga gcacaacggg gtgaaatgat
    12661 gaaacagctg gcgggctgcc tggtgatcct ctttgccgcc atgtgggcgg gctggctggc
    12721 ccatgactgg catgacgcca gggtggcgct tgccgaacag gaagccatgg aagccacgcg
    12781 acgtgcggta acagaagccg ggcagcagtc cgcccgcata ctggaaaaac ggctggaggc
    12841 gttaaagggc aatgaaattc acactgaacg cgttattcac acggaaaccg tccgcccggt
    12901 attccgcaat gtgtgcgcta ctgatgatta cgtccggctg ttcaacgccc gtgctgaagc
    12961 cgctgagcgt accctggcag gaaaaccagc tgacggaatg cccgaagaca ctgccgagac
    13021 tggcaggccc gacgggcgct gattttgatg cggcactgcg aaccctcacg agccgctaca
    13081 ccgaatgcgc cgccaggcat aacagcctgg tggaaaccat acgaaacacg gagaaactgc
    13141 aatgaccacg aagaacaccg gcaccatcat cctgtccgtt gcgggcgtaa ccctgagctt
    13201 tgaaccgaac aaaacggcat ttaacaacct catcaacgaa atcacgatga ccaacaaggt
    13261 ggcgccgatg agcacctatc ttaaccgcat cgtgacgcct gccagccgcg acgcactggc
    13321 tgacctgatt gcgcgttatc cgggctgtga gatgcagatc gtcgagaaga ttaacgaggt
    13381 ttactccccg aaactggaga ttgagttaaa aaactgaccg cgcgggtcga aagcatccgc
    13441 cgcaatcccc ttgagcagta tctggcattg cggcgtcact acctccccgg agagccggac
    13501 gacgaagaga gcatcgcccg tgccatctgg ctggatgaat attttgcccg tacccgcgcc
    13561 aacaagaccg ctgaaggtat agcgatcgca tttaacggaa ggtgacatgg ccagccagct
    13621 ggatttcatt atcaatctga cagaccagct tacccgcccg ctgcggcagg ttcaggaatc
    13681 cgtgacggga ttcgccgaac gctcgcaggg cgcgttcgcg cgtatcggtg tgggtgtggc
    13741 cggactgtgg ggcgtggccg aaggtatccg cgccgcgatg gatccggcgt gggagatgca
    13801 gaaggcgctg ggcgagcttg aggccaaagg tgtcggggcg caggcgctga ccgccctcac
    13861 cacgcaggcc atgaaactgg ggaccgcatt cggtcagtct tccacggaga tcatccgttc
    13921 cgcatggcat attcgcggcg cactggacgg gctgagcgac ggggatttac cccgtgtgac
    13981 ggcagcaacc agtaccctgg ccgtggctgc ccgcgtcagc agcgaagagg cgagcggcta
    14041 ccttgccgcc atgggtgccc gtttccgcag tgagttttct gccatgggtg ccgtggggtt
    14101 tgctgaagat ctggcaggta aaaccgcgtg gatggttcag cagttcggcg tggaaatgag
    14161 caccatgcag gagcttattg agggcaccaa aaacgcgggc gcggattttg gcgtcagcat
    14221 ggcagagcag tttgccatac tgggcaccct gtcacggacc atgggcaccg aggcatcggg
    14281 catctatgag gaattcctgc gcagtgcccc cgctgccgca caaaaaatgg ggatgagttt
    14341 tgttgatgcc accgggaaga ttttacccat gggtgacatc ctcgaccgtc tgcaggcgag
    14401 gttcggcagg aacatcgagg gtaacgtggc ggcacaacag gcgctggatg acgccttcgg
    14461 cagtggcgcg gacgtgatta aaaaactctg gggacagcag gaaatgctta accggagcat
    14521 atcagcgctg gggcgtaatg acggcatgaa gcgtgccacg gaaatggcgg aagccatgac
    14581 tgacccgtgg gaccgccttg tggcctcctt ctacaacatc cgggccgcca tcggtaacgc
    14641 cctctacccc attctcgcac cactggctgg ccgttttgcc gatatcggcg cgacgttcgt
    14701 gcgctggctg aacatgttcc cgaacatcgc ccgctggctg ggttacatca cgctgaccat
    14761 gctggcgctg ggcgcagccg gtgcggtcac gaacatcgtg ctgggtgtgt tcagcttcat
    14821 catggaaggc cataaggcca tcataaaggg cgccagggca gcctggagtc tgtacacgct
    14881 cgcactcaca aaagtacgca gcgtcatcat tgccaccagt cttgccgcgc gtctggcggg
    14941 cgtgtcgttt ctgaccatgg gcgccccggt cctcctggtt gttgccgcca tcgccgctct
    15001 ggtggtggtg gccattaagt tctggcagcc cgtcaaagcc tttattcgcg gctttattga
    15061 tggttttacc ggcgcactgg acgccatgag cccggtgact gccgcgttcg gcgacattgc
    15121 ccgggcgctg gccccggtat ggcagggcat aaaaaccctg ttcggtgcct tctccgatct
    15181 gtttgcaccg gtacagctga ccacgggaca gatgaacagc gtcacccgtg ccggggaaac
    15241 atgcggtcgc gtggtggccg cagcggtaag cctgatatcc cccgcgctgg gggtggttat
    15301 cagcgtgatc agaaacctga tcgacatctt caccatcgtc atcagagggt ggcaggacgt
    15361 ggtggctgca tttgacccga ccgccccggt cgaatccttc cggaaaatgg cggggatcat
    15421 cggtaacgtc ttcggggact tatggaacgt cattaaaaac tcgtttaacc ggacgtggaa
    15481 ctggcttgcg gaaaagctga acaaaatccc cggtatcagt atcgacatga agcccacgga
    15541 gcaggtgaac accatgacgc ggggcgcaac gggcagcacc gtacctccgc agattgcaga
    15601 aaccaccgtc ccggcactgg caggcaaccg catcagcgca gagctgccac ccggaggcat
    15661 acgcaaccag atacgcacgg acacctcaac ccgtatcgac aacagccgga cgttcggcaa
    15721 tctgacaatc aacacgcaga acccgatgac gccggggcaa ctggatgaat ggacggagct
    15781 ttacgcggga tgaacacgcc atcgctttac atcgacctgc ttatcactga cggcagtttt
    15841 acgctgaaca gcggaaacga accacagcga tgtgataacc gggtcagcat cggccaggac
    15901 gtggtgcaca gcattatcga aagcgggatc gccgccctgc ttatcggcga acgcagcccg
    15961 acgctgcgca gcgacatcat tacccggctt atcctcctgg ttgaggatga tgaacggctg
    16021 gttccgggga cggtgttcat taacgaagag gcaccgggca ggctgcatgt gacggcagag
    16081 acacaggatt ttggacgcat aaacaccagg gtgaactatg aaagggaaac cggacgttga
    16141 ttttaatcag gtattccgtg aaagcggtat gccggaaaca gaagaagaga tcgccaccct
    16201 ctttcatgag gatgtacaga aaaccggact catcaccaac acctcgccca tgagtccctt
    16261 ctggcggctc attctgacca ttgtcatacg acccgttcag tggctgcgtg atgcgctggt
    16321 tgacgttgtg ctgcccggta tgtttgtcgc cacggctgac cggtcacttc ttctgcttca
    16381 tgcgcacgac gtcaacatca ccccaaaacc ggccagtaag gccagtggcg tgatctggtt
    16441 tgccaaaacc agggcaaatg atgacgtcct aatccccgaa ggcaccatcg tccagaccgc
    16501 cagaattaac ggcgtgatat acagcatggc cgtcacggaa gaaacgctta tcagcagcgg
    16561 taaaagcggc gcatccgttc ccgtggaagc caccaccgaa ggcagcgggc acaatctcgc
    16621 gccggggtat tttcgtatcc ttcccgttgc cattcccggc attgcatccg tggtgaaccg
    16681 ggaggactgg ctgaccgtac cgggtgccga tgaggaaagc gacgacgaac tgcgcgacag
    16741 aatacgcaac cagtttaacc tcgtgagcag ttaccacacg gacgccgtct accggggact
    16801 tatcgccagc gtggcgggcc tgagcatcga ccggatctac tttcagcatg acgccccgag
    16861 aggaccggga acggccaacg cctacctgtt actggacagc ggcgaggcct ctgccccttt
    16921 cattgaagca gtcaaccgct acatcaacga agaggggcac cacggacacg gggatgattt
    16981 gcagtgtttc gccatgccgg agacgcgtca ccgtctggac gtcaccgcat acatcagaag
    17041 ttccaccacc ctgccaccgg acggcgcgga agggctgaaa caggatatcg gcgacctgat
    17101 acgctgcgct ttccgggaga acgcccgctt taatgtgaaa aaagtctggc cctattcgcg
    17161 cttttcgttt tccacgctgg gcaaggaaat tcatcagacc ttcccggtgg tggcttcggt
    17221 tcagttttca ctggaagaca tcatcagcga tctggccgtg ccacggctcg aatccctgac
    17281 agtggaggtg gtcattgaat gactttttgc agcggcttcg ctcgctgaaa ctgccctcat
    17341 ggatggacag gggagaaccc gacaaactgc tgcgggccgc ccgggcgttc tggtcccgcg
    17401 tatgggaatg gatcacctgg cccgtgcgcc agtttgaccc catgacatgt gcagagcctg
    17461 tacttgattt gctggcatgg gagcgggaca tcacccgctt taaaggggaa cccacggaga
    17521 tctaccgccg ccgcatcagt tacgccttca tcaatgcaca ggaggctggc gaggtggcgg
    17581 gctttatcgc catttttgaa cggctgggca tcggctacgt ggagttactc gagcgccagc
    17641 ccggcgtgga ctgggatgtg atcctggtgc gggtgacaga cagccagata gccgacaaca
    17701 gcgatcttct tctggagatc atccgcaaat atggcaggac atgccgccgt taccgttttg
    17761 aagtggaaac cctcaccccc ttcatcatcc attatggcgc atacgaaggg gagtatatct
    17821 gctggacggc cagggcagag tgaatgactg aaggagtatg actttatggc gaaatctgta
    17881 attaccagcg cctttatccg gctcaaggca cagcaggcgg cggaaggcac ccccgttgtg
    17941 ctggatgagt ttatttttgc aaacgtcccg gacctcaacc cggaaacgcc ggtatccccc
    18001 gacgaaaccc tgccgcctga agatcagatt gttcaccgcc aggccgttgg taaaaccggc
    18061 gtggtgaatg agaatgccgt ggtttattcg gtcaccctgg gggcagagct gggtgatttt
    18121 gaatttaact gggtgggact ggtcagcaaa gcaaccagca cgctggccat gattgttcac
    18181 gcccccacac agaaaaaaat cagaacccac aacggtaacg aaggtaatgt gctggtccgt
    18241 tccatgctca tggagtacag cggtgcgaag gaagccacag gtatcaccac cccggcgcag
    18301 acctggcaga tagacttcac ggcacgcctg gcagccatgg acgaacgcca gcgccgggaa
    18361 aacgtcgata tctacgggaa ggcaactttt tttgatgacg ggtggctggt taagaaaggc
    18421 gaaacctgga cagtcaacaa aggggtgggt tacgtggagg ggctacgcgc agagctgacc
    18481 gaagacacac caatggtcac gcccacctcc ccgtcccgta tctggctgaa tgtgtggtgg
    18541 accggtacgc tcaccagtga atggacggtg gagcacgaaa tcataccagc cgacacggac
    18601 gttacggagg agcgtttacg gaaaacatat cccagggacg gcattcagca atatttttat
    18661 gccatcgctg aaattgatga agacggcaac gtgactgacc tgcgccccag aggaaccccg
    18721 gaagagcgcc gcagtctgga cgccctgaaa aaacacgaga aatcacgcaa ccacccggac
    18781 gcaacgctga aagaaaaggg cttcacacag ttaagcagcg ccaccaccag tacatcagag
    18841 acacaggcag ccacgccaaa ggctataaag atcgccatgg acaacgccga tggtcggctt
    18901 gcgaaaaacc gcaatggtga ggacatcccc aacaaagagc tgttcctgat caacgtgggc
    18961 gcaccacgcg tttatgcaaa agacgggtac gtgggtgaag cagggcaacg ctggacaaca
    19021 gaacaattcg tggaatggct gaatgagcgt ggcgccttca gaccaggact ctggatattc
    19081 aaagccaata tttcactttc ccggcacagc gtgctcactg atacgggcta tggtgatatc
    19141 tgccttggag gctgccttat cgaagtatca ggcgacatca acggggggat catcaggatc
    19201 acaacaggtc tcgaaagccc aaaccctgcc gacatgtacc tccgcacaca gttcaccggg
    19261 gttgttttaa ataaagggaa aagcaccatc tggtaccgca cttacacgga caaaaacaaa
    19321 cccacaccac aggacatcgg cgcactgcct gcaagtgagt tgttacccgc aggcgttccc
    19381 ctgccgtggc cgtcagacac cccgcctgcc ggttacgcca tcatggtggg gcagacattc
    19441 gataaagcac gatatcccct gctggcaaaa gcctatccgg atggtcgtat cccggatatg
    19501 cgtggctgga caatcaaggg aaaacctgcc agcggtcgtg cggtattgtc acaggaacag
    19561 gacggcatca aatcccacac ccacgggggg agtgtctcaa atactgacct tggcacgaaa
    19621 acgacctcat catttgacta cggaaccaaa acaacctcta caaacggaca acatgaacac
    19681 agcgtccccc tgagaagaca agatggcgga ggcataaact ttgactggct ggatggttca
    19741 tcgtccggtt ctcatattgg taatggatac cttcctgccg caggtgacca ccaacatacc
    19801 gtacccattg gcgcacacac ccacacgctc gcactgggtt cacatggtca cggcctgacc
    19861 attaacccca ccggtaatgc cgaaaacacc gttaaaaata ttgcatttaa ctacatcgtg
    19921 aggcttgcat aatgaccttt aaaatgagcg aacacccaca gacaattaaa atctacaacc
    19981 tgtcagccgg aacaaatgaa tttatcggcg aaggcgatgc ctggatccct ccgcataccg
    20041 gactgcctgc gcactcaacg gacattgccc cgcctgacat tcctgaagga cacgtggcta
    20101 tctttgatga ggatgaaaac acctggcagc tcatggaaga tcaccggggt aaaaccgtct
    20161 ggcgtctgga gtcgggaagc gccttactga ttactgagtc gggcccgtta ccggaaaacg
    20221 tgaccataat aaaaccggac ggggagttct gccgctggga cgggtcatca tgggctaagg
    20281 atgaagcggc agaacaggct taccgggtga gcgaggcgga gaataaaaaa cgggaattaa
    20341 tacaggaagc aacaaataca ataaacatcc ttcaggatgc cgtggattta aatatggcga
    20401 ccgacgatga aagcgccctg cttcccctgt ggaaaaaata ccgggtatta ctgagccgga
    20461 cagacacggg caacgcgccg gatattgtct ggcctgaatc cccttcgccg gatcatcacg
    20521 caggctgacc ggaggcacca tgacgtggac aagaaaaaca atcagcgtca gcgctgacat
    20581 gcccccgctg agctgttccg tcatccccgt cacaccgtgg acttacgggc tgggacgata
    20641 tgaagaatcg ggggtatatt taagccctcc gaacgccata aactggctgg ccgggaaaat
    20701 ggccggaagc cgtgcatccg gcgacgtgac cattatcatg attgccgaaa acacccacga
    20761 tctttttata cagtcaatgg cggcactgac cgccgttctg cccgtgccgg tattcacgca
    20821 ggcacagcgc atggcacagg ctgccgccag tctgagcact gacaaaatgc aaatcccgat
    20881 caccaccgac accctgcccg cacctgtgca tctttccgtt gccacaatgc gcagcgcagt
    20941 cagtgccgcc cgtgcagcac aggccaggca gctggcagca caacgcccgg atgccgcagg
    21001 gcttcgccgc cagataagcc gctttatcag cagacgggct ggcgcacttg ccggactgga
    21061 agaggggatc agaacactgg cagaacagaa ggcgcaggcc tgggtatttc agtaccgggg
    21121 gtatcaccgc gccgcagcgg cggccatggt gaaggatatc ccggcaccga ccgccgtaca
    21181 taccgttgcc gcactcctgg cggcagactc attaactgaa ctggggaaaa tgatccatga
    21241 gccagaccgc actgctcgcc cttgatggtg agggtatttt catgcagaac atggtgattt
    21301 cgccctcgat gcagttccag gaaaaggacc agtccggcca gacatcgagc accgcgaacg
    21361 ccgagcaggg catcaaggcc aaagagcttc gcgtaaccgg gatgatcacc tttgacaatg
    21421 agcgggccct tcagcgcatt ttccagctgg cctctgccac caccggggac ggtgccctga
    21481 aagtttaccg tatcgcaaac gccaccgctg ccgccattaa ctttcgtgaa gggacatttt
    21541 ccgggcagat agacgcacag cagcagaccg accgcctcgc ctggcaggtc agcttcacat
    21601 tacgcgaaag acgaagcgta ccggaaaaac gccaggcacg cgccaccccc gccagcagcc
    21661 gtaaacagac gccgcagacg cccggcgcaa aaggcgggac cgtggccgac gaaacaccgg
    21721 agaaaatgac ctggttcgaa gaaaaggtcc tcaaacccgt caatgatgcg ctggagtaac
    21781 catgaacccc actaaacgtc tgttactggg taacgacacc attcagctgg tcagttgttc
    21841 cgtcatgctt gagctgaacg cgtgcggacg cggatttatc acagccagaa cagagcagaa
    21901 ctacaccggg cgccccgttc gtctcgatat cggatacgga aacgacctgg tgcgctggtt
    21961 taccggatac gttgaacgca gccagcccgc tgaaaacggc tcggtgcggc ttctgattcg
    22021 tgaaatggcg gggatccttg atcacccctt cccctgttca tttcagcacc cgacaatgcg
    22081 cacggtcaca cagtggctga gtgagaccag cgggctggaa atcatcctgc cggacaacgc
    22141 ggcttacact gacagacccg ttccgcattt cacacacagc ggcacgggct acgaactgct
    22201 ggccagtctg gggcgtattt tctccgtacc ggattacatc tggcatccgt taccggacgg
    22261 cggcattatg accgctgctg ccgcgcaggg gatgttttcc ggtcgccccg tcaccatccc
    22321 gcacgagttc agccaggcat caacgggcgg gcacagtatg accctcccga tgattcagac
    22381 gctgcgaccg ggtgtggagg tgaacggtca gcgcctgacg atggtcatgc tggaagatga
    22441 caacatgacc atcacgtgga caccacgcaa taaagccacc ggcgcccccc tccagaagtc
    22501 ccctttccgt cgccaggctg aaaaggcctt ccctgaactg gcatcagggc ttcacctgcc
    22561 caaactggca cgtgttgagg caccgaccga agccgtcagc gcgggcaata ttgccgaccc
    22621 gttcagaccc cgctacgcgg tgaatctcca gctgcttgac gagaacggca acccggcagc
    22681 agatacgccc gtctataacg ccgttcccct ccccgttccc atggcgggca gtgagtccgg
    22741 catgttccag ttcccgccac ccggaaccct ggtggaagtg ggatttgtgg aaggccgggc
    22801 ggataagccc ttcatccgcc agataatggc agagggccac aatctgcccg acgtgaagcc
    22861 gggcgagcag ttgcagcaac agcgcgacgg cgtatcgcag cgcgtgacgg tcgccggaga
    22921 ctgggagcgc cagacagacc agcggataca ggagacatca cgcgagcgcg tggtgagcgc
    22981 agacgaggaa aagcggagcc tgaccagccg ggagaccacc atacaggcaa ccgacacaac
    23041 caccgtcctg ggcgtatcca cgcttcaggc tggcgcggtc acccatatca ctgaagagaa
    23101 ctacagcatc agcacgggcg ggcacatgat ggtggttgcc ggtacctgtg agcgcgacgt
    23161 ggaaggcgac acccaggaca ccgtgggcgg cagccttacc gaaaggatta agggcgtccg
    23221 tcacagtatt gcgctggcgc agaagctggt tggcgatacg gtgaagctgg gaaacgaggg
    23281 cattaatgta ctgaccatgc tgacggacct tgccgatgtg gtggaagagc tggcagatat
    23341 tacggcatca catacccacc ccaaaacggg cacgtctccg caggccgcac agttcagcca
    23401 ggtcgctcag gagtgccgcc agcttaaaaa taaatattca ccaataatcg agtaattatt
    23461 cactttaaca ggccgcgcaa tgtggccttt tttattcccc cgacaataaa aaatattcat
    23521 caccggacat cacccgccac gaaaaaaccg tccgcagcag aaaaaaagat ggacgaaacg
    23581 ccccacgtaa tccccacgta aaacgtcgcg taacgccacg taacgaagaa ataatcccgc
    23641 cagcccgatt cgatcccctg acatcagaaa acgatttctg cgccccaaaa tcgccgcaaa
    23701 tggccggaaa ttttcggggg cgacgtaacg cgtaaagtga cgtaatcggc gctaccccgc
    23761 acacgcctcc ggtatttgtg tacgataaat tttgcaaatc gaaaggggtg caaaacaccc
    23821 ctccagggcg cgccattact gagtttttcc cttctgaatg gttttgcact ttgtgcaagg
    23881 ttttgcaaaa gtgtgcaaaa actccggctt tctgaggatc ccgaaaaatt ttaatgcact
    23941 gaataacaag agatttttaa aattacgtca cgatcgaaga tctgaaaaag gatccggtga
    24001 cgaaaacgga aaaatcagga aaaccagaac tggcgcggcc tggcggggag tggcagcaaa
    24061 aatcactttt gcacactttt gcacaactgc tccgaaaaag gatggcgaag gggaaggaaa
    24121 acagccggat catccgttgc acgtaacgga cgtaaaaggt aaattacgcg cgggtgcctt
    24181 tcggctgacg gctggaagga atacctgaag gccggatgtg aaaaggcccc gagtcaacta
    24241 acgttaaccc gaggccctga cacttcctac ctgacaagtg gaaggttagc gcctccctgt
    24301 aaaaggagtc aagcgctatg ccgcaaaaaa cgatcatagt gagcatttta tgcataacta
    24361 ttttgatgat tgtctggatg atacacaagt caccgtgtga gttcaggctt aacatcatgt
    24421 ggtcacaatt tgcggcgttc ttgcagtgta aacagtaaga aaccgcagcg ggggtgaaag
    24481 cccccgctat ccggttgccg ggttcggtcg atatggcacc cctttctcca ttggattaca
    24541 gacgtatttg cccgcaatat gcgggcgttt ttttaccgtc cccctcaagc attgcgcaca
    24601 caaagggaaa tcagtacatt acacgcgggt gcctttcggc tgatggctgg aaggattacc
    24661 tgaaggccgg atgtggaaag gccccggagt caactttaat gttaacccga ggccctgacc
    24721 atctacccta agcaagtgga aggttagcgc ctccctgtaa aaggagtcaa gcgctatgcc
    24781 gcaaaaaacg atcatcgtga gcattttatg cttaaccatg ttgttgactg tctggatgat
    24841 acaccgttct ccgtgtgagt tcagacttaa catcatgtgg tcagaatttg cggcgttctt
    24901 gcagtgtaaa ccgtaagaaa ccgcagcggg ggtgaaagcc cccgctatcc ggttgccaaa
    24961 ggttcggtcg aaagtcgcac cctcacacaa aaggcacaaa
//
LOCUS       DAZGQL010000078        25000 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.078, whole genome shotgun sequence.
ACCESSION   DAZGQL010000078 DAZGQL010000000
VERSION     DAZGQL010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25000
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.078"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(111..596)
                     /gene="sixA"
                     /locus_tag="ACHKIR_003561"
     CDS             complement(111..596)
                     /gene="sixA"
                     /locus_tag="ACHKIR_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HFO9991245.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            complement(799..2943)
                     /gene="fadJ"
                     /locus_tag="ACHKIR_003562"
     CDS             complement(799..2943)
                     /gene="fadJ"
                     /locus_tag="ACHKIR_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HFO9991246.1"
                     /translation="MEMASAFTLNVRLDNIAVITIDVPGEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIANCKTAQEAEALARQGQQLMAEIHALPV
                     PVIAAIHGACLGGGLELALACHGRMCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPQSILLEAAVELAKQDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSGGYDAEARAFGEL
                     VMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDRQLALISGTTDYCGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDTALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGTQGQGRLSAPQVAERCVM
                     LMLNEAVRCLDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMSKRGESFWKTTATDLQ"
     gene            complement(2943..4253)
                     /gene="fadI"
                     /locus_tag="ACHKIR_003563"
     CDS             complement(2943..4253)
                     /gene="fadI"
                     /locus_tag="ACHKIR_003563"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HFO9991247.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAIANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLAEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SDLTLLDMHEAFAAQTLANIQLLGSERFARDVLGRAHATGEMDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVVEAE"
     gene            complement(4434..4718)
                     /gene="yfcZ"
                     /locus_tag="ACHKIR_003564"
     CDS             complement(4434..4718)
                     /gene="yfcZ"
                     /locus_tag="ACHKIR_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HFO9991248.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKITPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            5090..6430
                     /gene="fadL"
                     /locus_tag="ACHKIR_003565"
     CDS             5090..6430
                     /gene="fadL"
                     /locus_tag="ACHKIR_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HFO9991249.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIRQSPAWLTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNPAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            complement(6492..7247)
                     /gene="mlaA"
                     /locus_tag="ACHKIR_003566"
     CDS             complement(6492..7247)
                     /gene="mlaA"
                     /locus_tag="ACHKIR_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HFO9991250.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            7541..8473
                     /gene="yfdC"
                     /locus_tag="ACHKIR_003567"
     CDS             7541..8473
                     /gene="yfdC"
                     /locus_tag="ACHKIR_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFO9991251.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKTIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            8549..8623
                     /locus_tag="ACHKIR_003568"
     tRNA            8549..8623
                     /locus_tag="ACHKIR_003568"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8582..8584,aa:Arg,seq:cct)
     gene            8803..8985
                     /locus_tag="ACHKIR_003569"
     CDS             8803..8985
                     /locus_tag="ACHKIR_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001316510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFO9991252.1"
                     /translation="MWRDSILHSAMHSLMNSQARDTRDTSLNDDNVLEISEPRIRSTF
                     ERLYQNMNEAFGLGDY"
     gene            complement(9065..10312)
                     /locus_tag="ACHKIR_003570"
     CDS             complement(9065..10312)
                     /locus_tag="ACHKIR_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9991253.1"
                     /translation="MALNIPFRNAYYRFASSYSFLFFISWSLWWSLYAIWLKGHLGLT
                     GTELGTLYSVNQFTSILFMMFYGIVQDKLGLKKPLIWCMSFILVLTGPFMIYVYEPLL
                     QSNFSVGLILGALFFGLGYLAGCGLLDSFTEKMARNFHFEYGTARAWGSFGYAIGAFF
                     AGIFFSISPHINFWLVSLFGAVFMMINMRFKDKDHQCIAADAGGVKKEDFIAVFKDRN
                     FWVFVIFIVGTWSFYNIFDQQLFPVFYAGLFESHDVGTRLYGYLNSFQVVLEALCMAI
                     IPFFVNRVGPKNALLIGVVIMALRILSCALFVNPWIISLVKLLHAIEVPLCVISVFKY
                     SVANFDKRLSSTIFLIGFQIASSLGIVLLSTPTGILFDHAGYQTVFFAISGIVCLMLL
                     FGIFFLSKKREQIVMETPVPSAI"
     gene            complement(10384..11298)
                     /locus_tag="ACHKIR_003571"
     CDS             complement(10384..11298)
                     /locus_tag="ACHKIR_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HFO9991254.1"
                     /translation="MSAKVWVLGDAVVDLLPESDGRLLPCPGGAPANVAVGIARLGGT
                     SGFIGRVGDDPFGALMQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVR
                     PSADLFLETTDLPCWRHGEWLHLCSIALSAEPSRTSAFTAMTAIRHAGGFVSFDPNIR
                     EDLWQDEHLLRLCLRQALQLADVVKLSEEEWRLISGKTQNDWDICALAKEYEIAMLLV
                     TKGAEGVVVCYRGQVHHFAGMSVNCVDSTGAGDAFVAGLLTGLSSTGLSTDEREMRRI
                     IDLAQRCGALAVTAKGAMTALPCRQELE"
     gene            11514..12947
                     /gene="cscA"
                     /locus_tag="ACHKIR_003572"
     CDS             11514..12947
                     /gene="cscA"
                     /locus_tag="ACHKIR_003572"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFO9991255.1"
                     /translation="MTQSRLHAAQNALAKLHERRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDENDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWTFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDSGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSISPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLVLWRYYPHENLDGYRSIPLPQGDMLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWQLG"
     gene            complement(12955..13950)
                     /locus_tag="ACHKIR_003573"
     CDS             complement(12955..13950)
                     /locus_tag="ACHKIR_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005088955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO9991256.1"
                     /translation="MASLKDVARLAGVSMMTVSRVMHNAESVRPATRDRVLQAIQTLN
                     YVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDILLAIEQTASEFGWNSFLINIFS
                     EDDAARAARQLLAHRPDGIIYTTMGLRHISLPESLYGENIVLANCVADDPALPSYIPD
                     DYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQGFEQAWRDAGRDLAEVKQFHM
                     ATGDDHYTDLASLLNDHFKSGKPDFDVLICGNDRAAFVAYQVLLAKGVRIPQDVAVMG
                     FDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEDREGGRVTRIPCPLLIRCST"
     gene            14193..14411
                     /locus_tag="ACHKIR_003574"
                     /pseudo
     CDS             14193..14411
                     /locus_tag="ACHKIR_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            14429..15760
                     /gene="dsdA"
                     /locus_tag="ACHKIR_003575"
     CDS             14429..15760
                     /gene="dsdA"
                     /locus_tag="ACHKIR_003575"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HFO9991257.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFPETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLEDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAEQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLTQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLHNATHLVWATGGGMVPEEEMEQYLAKGRS"
     gene            complement(15865..17403)
                     /gene="emrY"
                     /locus_tag="ACHKIR_003576"
     CDS             complement(15865..17403)
                     /gene="emrY"
                     /locus_tag="ACHKIR_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HFO9991258.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRLLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(17403..18566)
                     /gene="emrK"
                     /locus_tag="ACHKIR_003577"
     CDS             complement(17403..18566)
                     /gene="emrK"
                     /locus_tag="ACHKIR_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HFO9991259.1"
                     /translation="MEQINSNKKHSNRRKYFSLLAVVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIKSPVTGY
                     IAQRSVQVGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKDEDIAEMPELASTVTSMPAYTSKALVIDTTPIEKEISNIISHNGQL"
     gene            18982..19596
                     /gene="evgA"
                     /locus_tag="ACHKIR_003578"
     CDS             18982..19596
                     /gene="evgA"
                     /locus_tag="ACHKIR_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HFO9991260.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            19601..23194
                     /gene="evgS"
                     /locus_tag="ACHKIR_003579"
     CDS             19601..23194
                     /gene="evgS"
                     /locus_tag="ACHKIR_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HFO9991261.1"
                     /translation="MKFLPYIFLLCCGLWSTISFADGDYIEYRGISSNNRVTLDPLRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQRIRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALEDGEVDIVLSHLVASPPLNDDIAATNPLIITFPALVTTLHDSMRPLT
                     SSKPVNIARVTNYPPDEVIHQSFPKATIISFTNLYQALASVSAGQNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFLLTRKDSIVLNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPDLKVLENPYSPPYSMTDETGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLNPGGWDILPAAIYSEDRENNVSFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIKVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVQNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     ESPFKDVFSNTHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASEHAVYI
                     CGWQDITETRDLIHALEVERNKAINATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTLPERYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLG
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTIFTITIPVEITQQVAAVEAKAEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLNCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEITVFCQQND"
     gene            complement(23250..24395)
                     /gene="yfdE"
                     /locus_tag="ACHKIR_003580"
     CDS             complement(23250..24395)
                     /gene="yfdE"
                     /locus_tag="ACHKIR_003580"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HFO9991262.1"
                     /translation="MTNNECKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLARIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(24469..>25000)
                     /locus_tag="ACHKIR_003581"
     CDS             complement(24469..>25000)
                     /locus_tag="ACHKIR_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HFO9991263.1"
                     /translation="NAITIPIGLYLLNPSSGADGKKNSNLSALISAAKEPVVWAPVLA
                     TILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLTLAAHKFEFSAEIAYNTFLKLIL
                     MPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGIIIASRFNVYTRTGTASLAVSVLG
                     FVVTAPLWIYVSRLVS"
BASE COUNT         6947 a         6096 c         5621 g         6336 t
ORIGIN      
        1 attcgatcac aagcggatca ttgttagtac aataagattg cataaattca acattgccag
       61 aaaaaaataa aggcccgata gattacacca gcgggccttg tgcatgcgta ttaaatcgct
      121 tttgccatct tcagattaca cgggctcatc tgccagttga atgtgccgtt tccgctttca
      181 tcaagcgtta cgctggcaat ggccgaggtg gtaaacatag gcggcgtttc gcccggacat
      241 aactcggcaa ccagataccc gactaagggt aagtgggaga tcaccagtac cgaagcaaca
      301 ccttcattgg tcaacgcctg caaataggca ctgaccagac caacatcgcc gcagggcgtt
      361 aactccggca gaacttctgc actggaaggc aggttcagac aatcccctac ttcttcaagt
      421 gtttgctcgg ctcgcaggaa cgggctcacc agaacgcgtt cgatttccac tttttgacct
      481 ttcagccagt tcgccatcag gcgagattcg tcacaaccgt tagtggtcag aggacgaacc
      541 gaatcactgg cggcatcgag ggctgcgtcg ccgtgacgca tgataaaaac ttgcatattg
      601 caccgctttt gttaaccaga ttcgcccgtt ttctttcatc tgaaaccaga gaaagaaaac
      661 ggtggccggc attgtgcctt atccattcac cgaatgaaac gctgtttttt acctcaatgg
      721 cgtaagtata gtcaatatgc gtttacattt tgagcaacac tccgccattc agcgcggatt
      781 catatagctt tgaccttctt attgcaggtc agttgcagtt gttttccaaa aactttcccc
      841 acgcttgctc atctcgacta aacgctcgca aggggtaaaa cgggaaccat actgcgtggc
      901 cagtcgttgc attattgcaa ccacttcacc cgcaccgaga gaatcgatgt agcggaacgg
      961 accaccgaga aatggcggaa aaccaatgcc aaataccgcg ccaatatctc cgtcacgcac
     1021 gctacggata acctgctcgt ccagacaacg tactgcttca ttcagcatca acatcacaca
     1081 ccgttcagct acctgcggtg cggagagtcg cccctgccct tgtgtgccaa tgagcgggta
     1141 aatggcggga tcgacctgtt ttttgctttt acgccctttc tgaccataaa gatagaaacc
     1201 ccggccattt tttctgcctt tgcgatcgtc gttcaaaatt gaagaaacaa catttgcagg
     1261 cgcgctaaaa cgttctccat aagcggcttc cagtacagga ataattttag tcccggtgtc
     1321 gattcctacc tcatccaaaa gttggattgg gcctaccgga aaaccaaatt tcaccagcgc
     1381 ggtatcaatg tgctctaccc gttcaccttc ggtcaacatg cggatagctt cattaatgta
     1441 aggcgctaag atgcgattga cgtaaaaccc ggctttgtca cgcacgacaa ttggcgtttt
     1501 gccctgtttt ttcgccagtt ttactgtggt ggcgatggtt tgcgccgatg taccggcatg
     1561 gggaataatt tctaccaacg gcattttttc caccggactg aagaaatgca ggccaataac
     1621 ttgctcaggt cgcgcggcgt gagcggcgat atcgccaatc ggtaaagatg acgtgttcga
     1681 agcaaagatg gtatgagcgg cgcaattttg ctcaacttcc gccaccattt gttgttttaa
     1741 ttcgagattt tcaaacaccg cttcaataat cagatcgcga tgggcaaagc cgcaatagtc
     1801 cgtcgttccg gagattaatg ccagctgtct gtcacgttcg ctggctttga gatgacgacg
     1861 gcgaactttg ccctccagct gatcccaact gtacttcagc gcatgattta tgccctgcgg
     1921 gttgatatct ttaattctga ccggaagccc cgctttacaa gcagtgacat aagcaatacc
     1981 gccgcccatc aagccaccac ctaaaattcc cacgctgttt aatggcgcag gcggcgcatc
     2041 actgccagga tctttcttca cgtccgtact ggcaaaaaag atactacgca gcgcctgcga
     2101 ttgcggcgtc atcaccagtt cgccaaaagc acgggcttca gcgtcatagc cgccactggt
     2161 gccctgcgct aatcccgttt cgacaacctc caggatgcgt tctgtcgccg gatagttacc
     2221 ttgagttttg tgttctgttt tcttaccgac cattttgaac agcagcgcac gacctaacgg
     2281 ccccgccaga atacgctcgc gtacaggtag agggcgtgaa gatgggcgat cctgctttgc
     2341 cagctcaaca gcggcttcca gtaaaatgga ttgcggaacg acgtcatcca ccagccccag
     2401 ctttaatgcc tgtttcgccc gaagttgttt tccagtgagg atcatctcca atgctgtgct
     2461 gacacctatc aggcgcggta aacgctgggt gccgcctgaa ccgggtaata atccaagttg
     2521 tacttcaggc aaaccgagca ccgttttagg atcgtcagta cacatgcgac cgtggcacgc
     2581 cagcgccaac tccagcccac cgcccaggca agcgccatga atagccgcga taaccggaac
     2641 gggcaaagca tgaatctccg ccatcaactg ttggccctgc cgcgccagtg cttccgcttc
     2701 ttgcgccgtt ttgcagttgg cgatcatgtt gatgtctgcg ccagcaatga agttgtctgg
     2761 tttcgcggag ataaacacca cgccgcgcaa ctctttgttt tcacggagtt gcttaataat
     2821 ggcgcgcacc tgcgaggcaa actccgcctt cagggtattc attttctcac ccggtacgtc
     2881 gatggtgata acagcaatgt tgtccagacg gacattaagg gtaaacgctg atgccatttc
     2941 cattattccg cctccacaac cattgccgcg ccaagcccac ccgcagcaca ggcggtcact
     3001 aaaccaaatc caccgccgcg acggcgaagt tcatgcaatg tctgggtaat catccgcgcg
     3061 ccggtcgccg cgaagggatg cccgtaagca atcgaaccgc caagcacgtt aaatttgcta
     3121 tcgtccattt cgccagtggc atgtgcacgc cccagtacat cacgagcaaa acgttcgcta
     3181 cccagcaact gaatattcgc cagtgtctga gctgcaaagg cttcgtgcat atcgagcaat
     3241 gtcagatcgc tcattgtcaa accggcacgt tccagcgcca gcggcgttga ccaggccgga
     3301 ccgagcaaca tgtcctgcca gacatctatc gcagtaaatg cgtaactgcg cagatacccc
     3361 agcggtacca gccctaattc tttcgcccgc gattcggtca tcaggatcac cgccgctgcg
     3421 ccatcggtca gcggcgtact gtttgctgcc gttaccgtcc cgtgtttacg atcaaacgcc
     3481 ggacgcaact ttgcgtaatc agcaagcgag gaattaccgc gaatattgtt gtcttcggca
     3541 agcggttgtt tataaggagg gataaaggca gtcatcacct cttctttgag tttaccgtct
     3601 gaccacgcct gagcggctcg ctgatgcgaa cggtgcgcta atgcatcttg ctgttcgcga
     3661 gtaataccgt aggttttcgc catttgttca gcggtgtcgc ccatccgcaa gccagtagag
     3721 tattccgcca ctgccggagg caccggcatt aagtcgcgca aacgcaggcg agagaagagt
     3781 ttcaggcgct ggctcatggt tcgagctttg ttgacatcaa ccagcacgcg cgccagtttt
     3841 ttactgacgc caattggcaa tacggaagag gaatcggccc caccggcaat ccccgcgcga
     3901 atagttcccg ccatcaggct ttctgcgacg tttgcaatcg cctggaaact ggtagcgcaa
     3961 gcgcggctga cgctgtaagc atcggtatgc acattcattc cggtaccgag aacaatttca
     4021 cgcgcaatgt tgggggcttc aggcatttgt acgacctgac caaaaaccag ttgttcaatc
     4081 acttcggcgg ggatctcgct gcgtgccagc agttcgccta ccaccatctt ccccaaatca
     4141 acagcaggaa tgccatgaaa agccgtcgcc tggcgggcaa aaggcgtacg taaaccgcta
     4201 acaatggcga tacgatcgcc ctggcgggta accagcggta aaacctgacc cataacactc
     4261 ccctgtaaaa taataaataa agtggtctga cctgatcata gtcttaacca tttttttaca
     4321 tttagccaag tggagaaaag ggaaagtggg agctatgaca cagagagaaa agtagaagag
     4381 aaaagaaaac gcccctgcca gcaactgaca ggggcgtatg tacccgagag gaattaacgc
     4441 agacccagct ggaagatcag catttcagct tcgcaggcaa aagtgaaatc gatatccagg
     4501 cgcacaccgt cagactcttc agtgaaagtc ggggtgattt tgcacggttc ggattccacg
     4561 ctacgggctt tttcagtcag cgccgccagc gtttgttctg cttctgcgcg gtttgcaaac
     4621 acacggctgt aagacgcggt gcagtcggag ttgtccataa tggtgccaac atccatacag
     4681 cagcaaaccg gggtttcatc agcactacat ttactcatcg ttgatttcct ctgtatgtgc
     4741 acccaaggtg ccagataaac gttgtggata ttttacgctt ccggaaagtg ctgctccagt
     4801 tgttaattct gcaaaatcgg ataagtgacc gaaatcacac ttaaaaatga tctaaaacaa
     4861 aattcacccg aatccatgag tgcgccacct cccaattttg ccagctggat cgcgtttctt
     4921 agatcatatc tgaaaaaaga tagaaacata cttgcaacat tccagctggt ccgacctata
     4981 ctctcgccac tggtctgatt tctaagatgt acctcagacc ctacacttcg cgctcctgtt
     5041 acagcacgta acatagtttg tataaaaata aatcattgag gttatggtca tgagccagaa
     5101 aaccctgttt acaaagtctg ctctcgcagt cgcagtggca cttatctcca cccaggcctg
     5161 gtcggcaggc tttcagttaa acgaattttc ttcctctggc ctgggccggg cttattcagg
     5221 ggaaggtgca attgccgatg atgcaggtaa cgttagccgt aaccccgcat tgattaccat
     5281 gtttgaccgc ccgacatttt ctgcgggtgc ggtttatatt gatccggatg taaatatcag
     5341 cggaacgtct ccgtctggtc gtagcctgaa agccgataac atcgcgccta cggcatgggt
     5401 tccgaacatg cactttgttg caccgattaa cgaccaattt ggttgggggg cttcaattac
     5461 ctctaactat ggtctggcga cagagtttaa cgatacttat gcaggcggtt ctgtcggtgg
     5521 taccaccgac ctcgaaacca tgaacctgaa cttaagcggt gcatatcgct taaataatgc
     5581 atggagcttt ggtcttggtt tcaacgccgt ctacgctcgc gcgaaaattg aacgtttcgc
     5641 aggcgatctg gggcaactgg tggctggtca gattaggcaa tctcctgctt ggctgactcc
     5701 tcaggggcaa gcattagcag ctaccgccaa cggcatcgac agtaatacca aaatcgctca
     5761 cctgaacggt aaccagtggg gctttggctg gaacgccggt atcctgtatg aactggataa
     5821 aaacaaccgc tacgcattga cctaccgctc tgaagtgaaa attgacttca agggtaacta
     5881 cagcagcgat cttaacccgg cttttaataa ctacggtttg ccaattccta ccgcgacagg
     5941 tggcgcaacg cagtcgggtt atctgacgct gaacctgcct gaaatgtggg aagtgtcggg
     6001 ttataaccgt gttgatccgc agtgggcaat tcactatagc ctggcttaca ccagctggag
     6061 tcagttccag cagctgaaag cgacctcaac gagtggcgac acgctgttcc agaaacatga
     6121 aggctttaaa gatgcttacc gcatcgcgtt gggaaccact tattactacg atgataactg
     6181 gaccttccgt accggtatcg cctttgatga cagcccagtt ccggcacaga atcgttctat
     6241 ctccattccg gaccaggacc gtttctggct gagtgcaggt acgacttacg catttaataa
     6301 agatgcttca gtcgatgttg gtgtttctta tatgcacggt cagagcgtga aaattaacga
     6361 aggcccatac cagttcgagt ctgaaggtaa agcctggctg ttcggtacta actttaacta
     6421 cgcgttctga taacgcgttc gcttaataag aaaaaaggtg agttttgcga ctcacctttt
     6481 ttgtttgttt cttattcaga atcgatatct tttaaatcat cctgaatcgc ttgcgcgttc
     6541 ggattttcct gcggtttgag ttcgccgcca ttagcgatga aatcatgacg ctggaagtac
     6601 gcttcgcgca ccataatata aggatcggac gactgacgca gcagaccatc ggaatccaac
     6661 aactgcgcac gagtttctat cccttcaagc gtccatttac ccacagacat cggccaggtc
     6721 agccaggaaa gaaccgggta aagaccatcc gccatatcac caccgtcatc acgcagcgtg
     6781 aagctaccgt agaacggtaa ctgaacgtat ggcccataac ccacaccata atgaccaagc
     6841 gtactaccga agcggtgagg ttcagtccgt tgcagtttcg ggttcgccat ccctgcaaca
     6901 tcaataaagc cgcccatccc caaaatggtg ttcaggaaaa agcgggtaaa gtggaccatc
     6961 ccctgataag ggtcgccctg caagaagtag ttaaccatca ccgcaggttc ttcaaggttg
     7021 ccagtaaagt tgctcaaacc gttacgcgcc ggttgcggaa cataatcacg ccaggcgaca
     7081 gcgaccggtc gaacaatata cgggtctaat acattgaaat tgaagttgta catggtgcgg
     7141 ttgaaccctt ctagcgggtc agaacgccct tgctgatctg taccggaact cgcacacccc
     7201 accagaagcg tagttcccag agcaagcgcc gacaggcgaa gcttcataaa tgtctccctg
     7261 tttttttatg gcttatgcag tttgccatcc atgacggaac gataccgtat ccgcctgttt
     7321 aggtgtgggc gattgtaaca gcacgtcaac tgatgtccag acgccctgat ttgctgattt
     7381 gatcatagcc tggtaatcgc cgccctgtag gctacttgat tctatagaaa cagaaaaagg
     7441 caaacgcagc cttttctaca ttttcagagt aactcccgcc agttgcgagc aaaaaagccg
     7501 ctacgcttta gctatacgtg ctaattcaag agaagagacc atggacaacg acaaaattga
     7561 tcaacacagc gacgaaattg aagttgagag cgaagaaaaa gagcgcggca aaacaataga
     7621 aatagatgaa gatcgactcc cctcccgggc gatggcaatt catgagcata tccgccagga
     7681 tggtgaaaaa gagctggaac gcgacgcaat ggcactactg tggtcagcca ttgcggcggg
     7741 tctgtcgatg ggcgcttcgc tactggcaaa agggatattt catgtcgaac tggaaggcgt
     7801 gccgggcagc ttcttgctgg agaatctcgg ttataccttt ggttttatta tcgtcattat
     7861 ggcccgccag caattattta ccgaaaacac cgtgactgcg gtactacccg tcatgcaaaa
     7921 accgacaatg agcaacgttg gcttacttat gcggttatgg ggcgtcgtgc tgctgggtaa
     7981 tattctcggg acaggtattg cggcgtgggc atttgaatat atgccaatct tcaatgaaga
     8041 aactcgcgat gcatttgtca aaatcggcat ggatgtgatg aagaacaccc ccagcgagat
     8101 gtttgccaac gcgatcattt ccggctggct gatcgccact atggtttgga tgtttcctgc
     8161 agcgggtgcg gcaaagattg tggtgattat attgatgacc tggcttattg ccctgggtga
     8221 caccactcac atcgttgtcg gttctgttga aatcctctat ctggtgttta acggtacgct
     8281 gcactggagc gatttcatct ggcccttcgc actacctact ttagcgggga acatctgcgg
     8341 cggcaccttt atcttcgcgt taatgagtca tgcacagatc cgtaacgaca tgagcaacaa
     8401 gcgcaaagca gaagcacgcc aaaaagcaga acgtgcggaa aacattaaga aaaatgataa
     8461 aaacccggca taaatggcga gggtttaagc aatcgagcgg cagcgtactt accccgcagt
     8521 ccattagcgg gtatactcat gccgcattgt cctcttagtt aaatggatat aacgagcccc
     8581 tcctaagggc taattgcagg ttcgattcct gcaggggaca ccagataccc ttcaaaccaa
     8641 atctaccttc accccgtaaa agatgggttt ggcagcacac ttgccctata tctactcatt
     8701 tttactgcaa caggttgaaa tctcagcact gtcagaaagc gctgatgact aaacagccct
     8761 gggccgggcg atgtaaccat catacagaat cctgatagcg aaatatggcg tgactcgata
     8821 cttcactctg caatgcattc cttgatgaat tcgcaggccc gtgatacacg ggacacgtcg
     8881 ctgaatgacg acaatgtcct ggaaatcagc gaaccgcgta tccggagtac atttgagcga
     8941 ctgtaccaga acatgaatga ggcgtttgga ttaggtgatt attagcaggg gtaagcattt
     9001 ttgtattatt attttccggt tgagggatat ggagatatcg acaacaaccg gaaaaagttt
     9061 acgtctatat tgctgaaggt acaggcgttt ccataactat ttgctcgcgt tttttactca
     9121 ggaagaaaat gccaaatagc aacatcaggc agacaatacc cgaaattgcg aagaaaactg
     9181 tctggtagcc tgcgtggtca aagagtatcc cagtcggcgt tgaaagcagc acaatcccaa
     9241 gcgaactggc aatttgaaaa ccaatcagaa agatcgtcga cgacaggcgc ttatcaaagt
     9301 ttgccacgct gtatttgaag acggatatga cacaaagtgg aacctcaatg gcatgtaaca
     9361 gcttcactaa tgaaataatc caggggttaa cgaacagcgc gcaggaaagg atacgcaacg
     9421 ccataatcac aacaccgata agtaatgcat tttttggccc tacccgattc acaaagaaag
     9481 gaataatcgc catgcacagc gcttcgagta ccacctggaa tgagttgaga taaccataca
     9541 ggcgcgttcc tacatcgtgt gattcgaata aacctgcata aaagacagga aagagttgtt
     9601 gatcaaaaat gttatagaaa gaccacgtcc ccacaataaa tatgacaaaa acccagaagt
     9661 ttcgatcctt gaaaactgcg ataaaatcct ctttttttac ccctcccgca tccgccgcta
     9721 tgcactggtg atccttatct ttaaaacgca tgttgatcat cataaataca gcgccaaata
     9781 gcgagaccaa ccagaagttg atatggggac tgatactaaa aaatatacca gcaaagaacg
     9841 cgccaatagc atagccaaaa gatccccagg cgcgcgctgt tccatattcg aaatgaaaat
     9901 ttcgcgccat tttttcggtg aagctgtcaa gcaaaccgca tcccgccaga taccccaggc
     9961 caaaaaagag cgcccccaga attagaccta cagaaaaatt gctttgcagt aacggttcat
    10021 aaacgtaaat cataaacggt ccggtcaaga ccagaatgaa actcatacac cagatgagcg
    10081 gtttcttcag accgagttta tcctgaacga tgccgtagaa catcataaat agaatgctgg
    10141 taaactggtt gaccgaataa agtgtaccta attccgtccc tgttaatcct agatgtcctt
    10201 tcagccaaat agcgtataac gaccaccaca gcgaccagga aataaaaaag agaaatgagt
    10261 aactggatgc aaaacgatag tacgcatttc tgaatggaat attcagtgcc ataattacct
    10321 gcctgtcgtt aaaaaattca tgtcctattt agagataaga gcggcctcgc cgtttacttc
    10381 tcactattcc agttcttgtc gacatggcag cgctgtcatc gcccctttcg ccgttactgc
    10441 aagcgctccg caacgttgag cgagatcgat aattcgtcgc atttctcttt catctgtaga
    10501 taatcccgta gaggacagac ctgtgagtaa cccggcaacg aacgcatctc ccgcccccgt
    10561 gctatcgaca caattcacag acattccagc aaaatggtga acttgtcctc gataacagac
    10621 caccacccct tctgcacctt tagtcaccaa cagcatggcg atctcatact cttttgccag
    10681 ggcgcatata tcccaatcgt tctgtgtttt tccactgata agtcgccatt cttcttccga
    10741 gagcttgacg acatccgcca gttgtagcgc ctgccgcaaa cacaagcgga gcaaatgctc
    10801 gtcttgccat agatcttcac gaatattggg atcgaagctg acaaaacctc cggcatgccg
    10861 gatcgccgtc atcgcagtaa atgcgctggt acgcgaaggc tcggcagaca acgcaattga
    10921 acagagatgt aaccattcgc catgtcgcca gcagggcaag tctgtcgtct ctaaaaaaag
    10981 atcggcactg gggcggacca taaacgtaaa tgaacgttct ccttgatcgt tcagatcgac
    11041 aagcaccgtg gatgtccggt gccattcatc ttgcttcaga tacgtgatat cgacaccctc
    11101 agttagcagc gttctttgca ttaacgcacc aaaaggatca tcaccgaccc gacctataaa
    11161 cccacttgtt ccgcctaatc tggcgattcc caccgcaacg ttagctggcg cgccgccagg
    11221 acaaggtagt agccgcccgt ctgattctgg caagagatct acgaccgcat cccctaaaac
    11281 ccatactttg gctgacattt ttttccctta aattcatctg acttacgcat agtgataaac
    11341 ctctttttcg caaaatcgtc atggatttac taaaacatgc atattcgatc acaaaacgtc
    11401 atagttaacg ttaacatttg tgatattcat cgcatttatg aaagtaaggg actttatttt
    11461 tataaaagtt aacgttaaca attcacccaa tttgcttaac caggatgata aaaatgacgc
    11521 aatctcgatt acatgcggcg caaaacgcac tagcaaaact tcacgagcgc cgaggtaaca
    11581 ctttctatcc ccattttcac ctcgcgcctc ccgccgggtg gatgaacgat ccaaacggcc
    11641 taatctggtt taacgatcgt tatcacgcgt tttatcaaca tcacccgatg agcgaacact
    11701 gggggccaat gcactgggga catgccacca gcgacgatat gatccactgg cagcatgagc
    11761 ctattgcgct agcgccagga gacgagaatg acaaagacgg gtgtttttca ggtagtgctg
    11821 tcgatgacaa tggtgtcctc tcacttatct acaccggaca cgtctggctc gatggtgcag
    11881 gtaatgacga tgcaattcgc gaagtacaat gtctggctac cagtcgggat ggtattcatt
    11941 tcgagaaaca gggtgtgatc ctcactccac cagaaggcat catgcacttc cgcgatccta
    12001 aagtgtggcg tgaagccgac acatggtgga tggtagtcgg ggcgaaagat ccaggcaaca
    12061 cggggcagat cctgctttat cgcggcagtt cattgcgtga atggactttc gatcgcgtac
    12121 tggcccacgc tgatgcgggt gaaagctata tgtgggaatg tccggacttt ttcagccttg
    12181 gcgatcagca ttatctgatg ttttccccgc agggaatgaa tgccgaggga tacagttacc
    12241 gaaatcgctt tcaaagtggc gtaatacccg gaatgtggtc gccaggacga ctttttgcac
    12301 aatccgggca ttttactgaa cttgatagcg ggcatgactt ttatgcacca caaagctttt
    12361 tagcgaagga tggtcggcgt attgttatcg gctggatgga tatgtgggaa tcgccaatgc
    12421 cctcaaaacg tgaaggctgg gcaggctgca tgacgctggc gcgcgagcta tcagagagca
    12481 atggcaaact tctacaacgc ccggtacacg aagctgagtc gttacgccag cagcatcaat
    12541 ctatctctcc ccgcacaatc agcaataaat atgttttgca ggaaaacgcg caagcagttg
    12601 agattcagct gcagtgggcg ttgaagaaca gtgatgccga acattacgga ttacagctcg
    12661 gcactggaat gcggctgtat attgataacc aatctgagcg acttgttttg tggcggtatt
    12721 acccacacga gaatttagat ggctaccgta gtattcccct cccgcagggt gacatgctcg
    12781 ccctaaggat atttatcgat acatcatccg tggaagtatt tattaacgac ggggaggcgg
    12841 tgatgagtag ccgaatatat ccgcagccag aagaacggga actgtcgctc tatgcctccc
    12901 acggagtggc tgtgctgcaa catggagcac tctggcaact gggttaacat aatatcaggt
    12961 ggaacaacgg atcaacagcg ggcaagggat ccgcgtcact cttccccctt cacgatcttc
    13021 aataatatgc aatgcagctt cccgcccgat aatgtcatgt ggaagctgaa ttgtggtcag
    13081 cggcggtaaa aacagatgcc cgacgccaac cagattatca aagcccatta cggcgacatc
    13141 ctgcgggata cgtaccccct tcgccaggag aacctgataa gcgacaaagg ctgcgcgatc
    13201 gttaccacat atcagaacat caaaatctgg tttgcccgat ttgaagtggt cattgagtaa
    13261 acttgcgaga tcggtgtagt gatcatcacc tgttgccatg tgaaattgtt tcacctcagc
    13321 cagatctcgt ccagcatcac gccaggcctg ctcaaatccc tgccgacgat accctgttgc
    13381 caacgcactt tccggtagcc agaagcataa cggttgacga tagcccgccg cgagcaaatg
    13441 ctgtgttgat tcatattgtg cagtgtaatc atcagggata taactgggta acgctgggtc
    13501 atccgccaca cagttcgcca atacaatatt ttcaccatac agagactcag gcagcgagat
    13561 atgtcgcagc cccattgtag tatagataat gccatccgga cggtgggcaa gcagctgacg
    13621 tgccgcgcgg gcagcgtcat cttcagaaaa aatattgatt aaaaaactat tccagccaaa
    13681 ctcgctggcg gtttgctcaa tggcaagcag aatatcaaca gagaaaggag tggtggccgt
    13741 gtcctgcgcc agcacggcga gagtcgacgg cttacgtcct tgagcgcgca tcttacgggc
    13801 ggaaagatca ggaacataat tcagggtctg gattgcctgc aatacgcggt cacgcgttgc
    13861 aggacgcaca gattctgcat tatgcatcac ccgggagact gtcatcatcg acactcccgc
    13921 caggcgtgcg acatccttta atgaagccat acccaagccg tttgccgtaa aacgggcact
    13981 gtagcagaaa cagacgtcac tggcgagatc caacgatatt ctcaacgccc tatcacccga
    14041 cacggcaata caataaaaaa taacaataat tcccggacaa ttgtacccaa ttccgcctct
    14101 gttctcgcca acgagtaacc atgtaggcaa gtaggtaaaa catgccaact ggcatcgcca
    14161 acctggagaa gaaaatataa atatggatca ttgtgctgcc gctgtatccc gacatcagtc
    14221 cggaaattat tgcgattgct atcggttcag gtgcgattgg ctgcacgatc gttacggact
    14281 cgcttttctg gctggtgaag caatattgcg gcgctacgct caatgaaaca tttaaatact
    14341 atacgacagc gacatttatc gcttcagtcg tcgctctggc gggcacattc ctgctgtcat
    14401 ttatcatcta agcgcaaaga gacgtactat ggaaaacgct aaaatgaact cgctcatcgc
    14461 ccagtatccg ttggtaaagg atctggttgc tcttaaagaa accacctggt ttaatcctgg
    14521 cacgacctca ttggctgaag gtttacctta tgttggcctg accgaacagg atgttcagga
    14581 cgcccatgcg cgcttatccc gttttgcacc ctatctggca aaagcatttc ctgaaactgc
    14641 tgctactggg gggattattg aatcagaact ggttgccatt ccggctatgc aaaaacggct
    14701 ggaaaaggaa tatcaacaac cgatcagcgg gcaactgcta ctgaaaaaag atagccattt
    14761 gcccatttcc ggctccataa aagcacgcgg cgggatttat gaagtcctgg cacatgcaga
    14821 aaaactggct ctggaagcgg ggttgctgac gcttgaagat gactacagca aactgctttc
    14881 tccggagttt aaacagttct ttagtcaata cagcattgct gtgggctcaa ccggaaatct
    14941 ggggttatca atcggcatta tgagcgcccg cattggcttt aaggtgacag tacatatgtc
    15001 tgctgatgcc cgggcatgga aaaaagcgaa actgcgcagc catggcgtta cggtcgtgga
    15061 atatgagcaa gattatggtg ttgcagtcga ggaaggacgt aaagcagcgc aatctgaccc
    15121 gaactgtttc tttattgatg acgagaattc ccgcacgttg ttccttgggt attccgtcgc
    15181 tggccagcgt cttaaagcgc aatttgccga gcaaggtcgt attgtcgatg ctgataaccc
    15241 tctgtttgtc tatctgccgt gtggtgttgg cggtggtcct ggtggcgtcg cattcgggct
    15301 taaactggca tttggcgatc atgttcactg cttttttgcc gaaccaacgc actccccttg
    15361 tatgctgtta ggcgtccata caggattaca cgatcagatt tctgttcagg atattggtat
    15421 cgataacctt accgcagcgg atggccttgc agttggtcgc gcatcaggct ttgtcgggcg
    15481 ggcaatggag cgtttactgg atggcttcta tacccttagc gatcaaacca tgtatgacat
    15541 gcttggctgg ctgacgcagg aagaaggtat tcgtcttgaa ccttcggcac tggcgggtat
    15601 ggccggacct cagcgcgtgt gtgcatcagt aagttaccaa cagatgcacg gtttcagcgc
    15661 agaacaactg cataatgcca ctcatctggt gtgggcgacg ggaggtggaa tggtgccgga
    15721 agaagagatg gagcaatatc tggcaaaagg ccgttcataa cgtttcaacg cagcatcgcc
    15781 atcctttccc tgggtgagcg atgctgccga tggcgcagac ttaagatccc cggtcttacc
    15841 tgctataatt ctctctttat accatcaccc aacgcctttc gctgtaaacg gcggtttcgc
    15901 aaaccaaacc aataccgtaa gtaagataaa agcaatagcc gccatacgga aaatctcatt
    15961 tgcagaaatt gaaagtgact gctgagttat ttcattatta atttcattaa gaactcctga
    16021 aagcgaacca tagtatttat ccataatttg tgacgatgaa ttaaatacgg ggttaaattg
    16081 atcgatggtt gctgtcaact gactatggtg taacgattcg cgtcgtcccc acagcgtcat
    16141 tgtcaacgac gtaccaactg atcctgacaa ggtacgaaaa aaattactca tactcgaggc
    16201 attggcaaat ttattatctg gcaaccctga aaacgaaatc gttgttaagg gtaaaaagaa
    16261 acaggcaacg gcgaatccct ggaaaaactg tggcaaaatg atgcctgtaa aatcaatcgt
    16321 tggcataaat gtcacagaac gccagtaata gcaaaccgca tacatcaaaa aactaaatgt
    16381 caccaacaac cgcatatcaa ttttgttgcc ataacgtcct atcaaaggtg atattaatag
    16441 cggcatgatg ccgatgggcg cataggcaag tccggcccat atcgcattat accccatcgt
    16501 ttcctggagt aactgcggca taaggactat cgccccagag taaaataaat acgcgcatgt
    16561 gatactcaca ataccaatgg tgaaattacg ggacttaaac aaactgagat caagaatcgg
    16621 attctctgag gtcgactccc aaatgactaa agaaatcaga gaaataactg acactactgt
    16681 tagtattatt attgtactcg agttgaacca atccagatcg cgccctttat caagcattat
    16741 ctgcaagcca ccaacaccga gcactaacag ggtcagtcct ggtagattca ttttgaccgg
    16801 tgaagtctca gtttctcttc ctttaagtaa ggttaagcat aatgtcagaa cgataatccc
    16861 catagggaca ttgattaaaa atatccaacc ccagctaaag ttatcgcaaa tatagccgcc
    16921 caatatcggc ccacatatcg gagcgataat tacggtcatt gaccataatg ccaaagcaaa
    16981 tgttcttttt tctggcggat aattccttaa taataaactc tgtgacagtg gaattaacgg
    17041 ccccgccatt aagccctgaa cgactctaaa aaatatcagc acatcaagat tggtcgatag
    17101 gctacacatt aatgaagata gtgaaaaaaa ggttactgaa agtaaaaata atcttaattc
    17161 gcctattctt tgtgccagcc tgccggtaac agggatcgca atggcatttg ctacgccaaa
    17221 cgaagtgata acccaggtgc cttcgtctgt tgatgctccc agaaagccag atattgtcgg
    17281 tattgcgacg ttagaaatag tggaatccaa catttgcata aacgtcgcta aggacaatgc
    17341 aatagtaacg caccataacg tcccaccggt taatggtgcc ggagttgatt tagtgattgc
    17401 cattaaagtt gtccattatg cgaaataatg ttgctaattt ctttttctat cggagtagta
    17461 tcgataacta aagccttact ggtataagca ggcatggagg tcacggttga agccagctca
    17521 ggcatctcag caatgtcttc gtccttcgta tcaatagttg cagtcatcga taaaccaata
    17581 cgtaagggat gttccatgag ttcttttgga tcaagagaaa cttcaaccgg tacacgctga
    17641 acgattttga tccagttccc tgtcgcattt tgtgcaggta ataaggagaa cgcattgccg
    17701 gttcccatat tgattcctgt cacccgacca tgaaacacaa cattttcacc ataaagatcg
    17761 ctgataatat tgaccgattg accaatccgt acatccgtga gttgtgtttc tttaaagttg
    17821 gcattaaccc acatttgacg tgccggtacg acagccatta acgattgtcc gggactcact
    17881 gtttcgccga cctgaacact tcgctgggca atatagccgg taacaggact cttaatatcc
    17941 gtacgtttaa gcgctaacca ggcttctttg gttgcatctg ccgcttcaac gacttgtggc
    18001 tgacggttta atggtgtgtt cattactaaa gctttattcg ctttataagc ctggatagcg
    18061 gcattcaatg ccgctttgct acttattaac gtatctttgg tatgctccag cgtttctttt
    18121 gaaataaccc cctgcttcgc taaaggcaca cgacggttat aatcttctaa agattgttga
    18181 tactgaatac gtgctgaagc gacttcggca ctgtattgtt tatcctgtaa gtacagttta
    18241 ttcgtttgcc gaacaatatt tgccagatta tttttagctt tattgagtgc gatagtggca
    18301 tcagtcttat ccagtgagac taaaatgtca ccctgtcgaa cgtagttcgt atctttatga
    18361 ttaacgacag tgacgctgcc tgaaacttgt gcagaaattg gatctgcatt ccccgtgaca
    18421 taggcgtcat ctgtactaat catgtcttct aattccattg accaataggc ataggcacct
    18481 gaaaacgcaa taaataaaac taccgccaat aaagaaaagt attttcttct gttagaatgt
    18541 tttttatttg aattaatctg ttccactttt atctctcatt tcgcatagat gaaatttatg
    18601 aattgaatga aaagagtatt cattcttcat atcaaacaca gtgcatgtgt tgcacattat
    18661 cccaggaacg ttggaggcct aatttaaatg aggtgcaaaa aaaacaacag aggactaaac
    18721 cgtggctttt gcaatacaat tcttacgcat gtaggattag tgagaagact tatagtgcta
    18781 atgtgaaact ataaatcatc ggtacaatcc ctgattttat tgttgacatt tcatttatgc
    18841 cgactattta tatggtatac ttgttgaatt atcttaaagg aagctcaaat tttcttattt
    18901 tgattgagaa aatgagatga tgccttatgt ctgtattact acagggagaa gggaaatgct
    18961 tcattgcaaa gggaataatc tatgaacgca ataattattg atgaccatcc tcttgctatc
    19021 gcagcaattc gtaatttatt gatcaaaaac gatattgaaa tcttagcgga gttgactgaa
    19081 gggggaagtg ccgttcagcg ggtggaaaca cttaaacctg atatcgtcat cattgatgtc
    19141 gatatccccg gagttaacgg tatccaggtg ttagaaacgc tgaggaagcg ccaatatagc
    19201 ggaattatta ttatcgtctc cgctaaaaat gaccattttt acgggaaaca ttgtgctgat
    19261 gctggcgcta atggtttcgt gagtaaaaaa gaaggcatga acaatatcat tgcagctatt
    19321 gaagctgcaa aaaatggcta ctgctatttc cccttctctc tcaaccggtt tgttggaagt
    19381 ttaacgtccg accagcaaaa actcgactcc ttatcgaaac aagaaattag tgtcatgcgg
    19441 tatattcttg atggcaagga taataatgac attgctgaaa aaatgttcat cagcaacaaa
    19501 actgtcagca cttataaaag tcgcttgatg gaaaaattag aatgcaaatc actgatggat
    19561 ctttacacat tcgcacaacg taacaaaatc ggctaaccac atgaagtttt taccctatat
    19621 ttttcttctc tgttgtggtc tttggtcgac cataagtttc gcagacggag attacatcga
    19681 atatcgtggc atcagtagta acaaccgtgt cacacttgat ccactacgtc tgagcaacaa
    19741 ggaattacgt tggctcgcga gcaaaaaaaa tcttgtgatt gcagtacaca agtcccaaac
    19801 agctacgttg ttgcataccg attcgcagca acggattcgt ggtattaatg ctgattattt
    19861 aaatctttta aaaagagcgt taaatatcaa attaacactc cgggaatacg cagatcatca
    19921 aaaagcaatg gacgcgctgg aagacggtga agttgatata gtgttatcac atttagttgc
    19981 ttcgccgcct cttaatgatg acattgctgc aaccaatcct ctgataatta cctttccggc
    20041 gctggtcact acccttcacg attcaatgcg accgcttacc tcatcaaaac cagtaaatat
    20101 tgctcgagta acaaattatc ccccagacga ggtaattcat caatcatttc caaaagcaac
    20161 aattatctct tttacaaatt tatatcaggc attagcatcc gtctcagccg gacagaatga
    20221 ttactttatt ggtagtaaca tcattaccag cagtatgatt tcccgttatt ttactcactc
    20281 cttaaatgta gtgaagtatt ataactcacc gcgtcaatat aactttttat taaccagaaa
    20341 agattctatc gttcttaatg aagtactcaa tcgatttgtt gatgctttaa caaatgaagt
    20401 tcgctatgaa gtatcacaaa attggcttga tacaggaaat ctggcctttc tgaacaaacc
    20461 attagaactc actgaacatg aaaaacagtg gattaagcag catcccgatt taaaggtgct
    20521 agaaaatcct tactcgccac cctattctat gacagatgaa actggctcag ttcggggcgt
    20581 gatgggtgat attcttaata ttattacctt gcaaacaggt ttaaattttt ctccgatcac
    20641 cgtttcacac aatattcatg ctgggacaca gcttaacccc ggtggatggg atatattgcc
    20701 cgccgctatt tatagtgaag atcgagaaaa taatgtttca tttgctgaag ccttcataac
    20761 aacgccttac gtttttgtca tgcaaaaagc gcctgacagt gaacaaacat taaaaaaagg
    20821 aatgaaagtt gccattccct attattatga gcttcattcg caattaaaag agatgtatcc
    20881 ggaggtagag tggataaaag tcgataacgc cagcgctgca tttcacaagg ttaaagaagg
    20941 tgaacttgat gctctggtcg cgacacagtt aaattcgcgt tacatgatcg accattacta
    21001 tcctaatgaa ctttatcatt ttcttattcc cggcgttcag aatgcatcac tttcgttcgc
    21061 ttttcctcgc ggagaaccgg aacttaagga tattattaat aaagcactga atgcaattcc
    21121 cccaagcgaa gttctgcgcc tgaccgaaaa atggattaaa atgcccaatg tgaccattga
    21181 cacatgggac ctctatagcg agcaatttta tattgttacg acattatccg ttttattagt
    21241 tggcagtagc cttttatggg gattctacct gttacgctca gttcgtcgtc gtaaagttat
    21301 tcagggtgat ttagaaaacc aaatatcatt ccggaaagcg ctctcggatt ccttaccgaa
    21361 tccaacttat gttgtaaact ggcaaggtaa tgtcattagt cacaatagtg catttgaaca
    21421 ttatttcact gctgattact ataaaaatgc aatgttacca ttagaaaaca gtgaatcccc
    21481 ctttaaagat gttttttcta atacgcatga agtcacagca gaaacgaaag aaaaccgaac
    21541 aatatacaca caggtatttg aaattgataa tggcatcgag aaaagatgca ttaatcactg
    21601 gcatacctta tgtaatctgc cagcaagcga acatgctgtt tatatttgtg gttggcaaga
    21661 tattaccgag acgcgtgatt taattcatgc actcgaagta gagagaaata aagcgatcaa
    21721 tgcaactgtc gcaaaaagcc agtttctggc aacaatgagt cacgaaataa gaacaccaat
    21781 aagctccatt atgggcttcc tggaacttct gtcgggttct ggtcttagca aggaacaacg
    21841 ggtggaggcg atttcacttg cctacgccac cggacaatca ctcctcggct taattggtga
    21901 aatccttgat gtcgacaaaa ttgaatcggg taactatcaa cttcaaccac aatgggtcga
    21961 tatccctact ttagtccaga acacttgtca ctctttcggt gcgattgctg caagcaaatc
    22021 gatcgcatta agttgcagca gtacgcttcc tgaacgctac ctggttaaga tcgaccctca
    22081 ggcgtttaag caggtcttat ctaatttact gagtaatgct ctcaaattta ctaccgaggg
    22141 ggcagtaaaa attacgacct ccctgggtca cattgatgac aaccacgctg ttatcaaaat
    22201 gacgattatg gattctggaa gtggattatc gcaggaagaa caacaacaac tgtttaaacg
    22261 ctatagccaa acaagtgcag gtcgtcagca aacaggttct ggtttaggct taatgatctg
    22321 caaagaatta attaaaaaca tgcagggcga tttgtcatta gaaagtcatc caggcatagg
    22381 aacgatattt acgatcacaa tcccggtaga aattacccaa caagtggcgg ctgtcgaggc
    22441 aaaagcagaa caacctataa cactacctga aaagttgagc atattaatcg cggatgatca
    22501 tccgaccaac aggctattac tcaaacgcca gctaaatcta ttaggatatg atgttgatga
    22561 agccactgat ggtgtgcaag cgctacacaa agtcagtatg caacattatg atctgcttat
    22621 tactgacgtt aatatgccga atatggatgg ttttgagttg actcgcaaac tccgagagca
    22681 aaattcttcc ttacccatct gggggcttac agccaacgca caggctaacg aacgtgaaaa
    22741 agggttaaat tgcggcatga acttatgttt gttcaaaccg ttgactctgg atgtactgaa
    22801 aacacattta agtcagttac accaggttgc gcatattgca cctcagtatc gccaccttga
    22861 tatcgaggcc ctgaagaata atacggcgaa tgatctacaa ctgatgcagg agattctcat
    22921 gactttccag catgaaacgc ataaagatct acccgctgcg tttcatgcac tagaagctgg
    22981 cgataacaga actttccatc agtgtattca tcgcatccac ggtgcggcta acatcctgaa
    23041 tttgcaaaag ttgattaata ttagccacca gttagaaata acacccgttt cagatgacag
    23101 taagcctgaa attcttcagt tgctgaactc tgtaaaagaa cacattgcag agctggacca
    23161 ggagattact gttttctgtc agcaaaatga ctaaatagcg gctcccacaa tgttcaaatg
    23221 tgggagctat ttaccagcac atcttacatt tatgatgaga actcctggcg aatttgttcc
    23281 ccatgctggt cgagcgttgc cgctcccggc ataacatgcg ggtccgcgca gccgctgatt
    23341 tttatcggat tccccggcat cattattccc ccggcttcaa tcaacatatt tctcgcctga
    23401 gtttgtggca aattaatggc ctcagccaca cttaataacg gcgcgacggg tacaccaact
    23461 tcatgtattc tggctaacca aacttcagct gcctgcgttt ttaacgtccg ctcaatatat
    23521 tgtttaagaa tagcctggtt ttgtacgcgt aaaatattgc tgctaaatct gggatcatta
    23581 accagttccg taagctccag tgcctggcat aacgcagaaa aaagcttgtc attaccacaa
    23641 caaatcgtaa tcggcttatc ctgagtatcg aaaacatcaa aaggtgccat gtagggatgg
    23701 cgatttccca ggcgttgtgg tgacttccct gtcgcaatat atgccatcag accatgctcc
    23761 agaaaactca gcgtggcatc aaacatcgct atatcgacat gcgcccctct ttggctcttt
    23821 tcgcggccat aaagtgcact cactattccg ctgaataaat agacaccgcc gcatagatcc
    23881 gcaagagagg tacccacgcg cactggcgga gcatcagggt atcctgtttc catcattatc
    23941 ccgctcattg cctgaatgat ggtatcgtag gcaggagcat cttttagcgg accggtatgt
    24001 ccgaaacctg acgatgaagc atatataagg cgcgggttga tttcttgtaa cctttcccat
    24061 gaaaacccga gtttttccat tgtacctggg cgaaaattct cagctaatac atccgcttgt
    24121 ttaagcatat ttataaatat acttttatcg tgatcattct ttaaatcaag aaccacactc
    24181 tctttgccat gattaataaa actgtaatag agtgactgtc catccacata gggaccaaat
    24241 gtgcgggtat catcaccatg acccggcggc tcaactttaa ttacccttgc gcccatatta
    24301 caaagaagtt gagttccgaa aggcccatta aggacatgcg tcatatcgat aactaataag
    24361 ccttcaaacg gccctttgca ttcattattt gtcatttgct tatgccttga aaaaagccga
    24421 aggtgaaaag acacttcggc atttatgtaa aatcaataac atgattaatt atgaaaccag
    24481 tcgactgaca taaatccaca aaggagccgt gacgacaaaa cccagtacgc tcacagccaa
    24541 tgacgctgta ccagtgcggg tgtagacatt aaaccggctg gcaataatga ttccggagaa
    24601 tgccggcggt aaggcgcctg ccagtaccat catctgcaaa tgttcgctgt tcaaatgaca
    24661 tgccatacca acgagaagca gcgccagtgg catcagaatc agcttcagga aggtgttata
    24721 agcaatttcg gcactgaact cgaatttatg tgcagccaga gtcaaccccg cagcgaatac
    24781 cgctacccct gagttagcct tcgcaatcag attaaaggtt gggtcccatg ctgccggaat
    24841 ttttacccca accaacacca ggatcgttgc cagaacaggt gcccatacta ctggctcctt
    24901 tgccgcagaa attaatgcgc tcagattact attcttctta ccatccgctc ctgaggaagg
    24961 attcagcaaa tacagaccaa taggaatagt aattgcatta
//
LOCUS       DAZGQL010000079        24667 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.079, whole genome shotgun sequence.
ACCESSION   DAZGQL010000079 DAZGQL010000000
VERSION     DAZGQL010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24667
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.079"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(206..397)
                     /gene="yfgG"
                     /locus_tag="ACHKIR_003582"
     CDS             complement(206..397)
                     /gene="yfgG"
                     /locus_tag="ACHKIR_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgG family protein"
                     /protein_id="HFO9991264.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            415..567
                     /locus_tag="ACHKIR_003583"
     CDS             415..567
                     /locus_tag="ACHKIR_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9991265.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     YVIMMS"
     gene            749..2992
                     /gene="pdeF"
                     /locus_tag="ACHKIR_003584"
     CDS             749..2992
                     /gene="pdeF"
                     /locus_tag="ACHKIR_003584"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HFO9991266.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGIALGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDSRLISQRLFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAI
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQRALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELISPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEISQLLAKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(3031..4572)
                     /gene="ppx"
                     /locus_tag="ACHKIR_003585"
     CDS             complement(3031..4572)
                     /gene="ppx"
                     /locus_tag="ACHKIR_003585"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HFO9991267.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(4577..6643)
                     /gene="ppk1"
                     /locus_tag="ACHKIR_003586"
     CDS             complement(4577..6643)
                     /gene="ppk1"
                     /locus_tag="ACHKIR_003586"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HFO9991268.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            complement(6814..7452)
                     /gene="purN"
                     /locus_tag="ACHKIR_003587"
     CDS             complement(6814..7452)
                     /gene="purN"
                     /locus_tag="ACHKIR_003587"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFO9991269.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            complement(7452..8489)
                     /gene="purM"
                     /locus_tag="ACHKIR_003588"
     CDS             complement(7452..8489)
                     /gene="purM"
                     /locus_tag="ACHKIR_003588"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFO9991270.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLKLIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            8814..9440
                     /gene="upp"
                     /locus_tag="ACHKIR_003589"
     CDS             8814..9440
                     /gene="upp"
                     /locus_tag="ACHKIR_003589"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFO9991271.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            9526..10815
                     /gene="uraA"
                     /locus_tag="ACHKIR_003590"
     CDS             9526..10815
                     /gene="uraA"
                     /locus_tag="ACHKIR_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HFO9991272.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISMLRPEEVVLDAEDADITDK"
     gene            10910..11611
                     /gene="hda"
                     /locus_tag="ACHKIR_003591"
     CDS             10910..11611
                     /gene="hda"
                     /locus_tag="ACHKIR_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HFO9991273.1"
                     /translation="MNTPAQLSLPLYLPDDETFASFWPGDNSSLLAALQNVLRQEHSG
                     YIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVPEVLDGMEHLSLVCID
                     NIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNLGLPDLASRLDWGQIY
                     KLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITA
                     QRKLTIPFVKEILKL"
     gene            complement(11717..12076)
                     /gene="arsC"
                     /locus_tag="ACHKIR_003592"
     CDS             complement(11717..12076)
                     /gene="arsC"
                     /locus_tag="ACHKIR_003592"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HFO9991274.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(12097..13560)
                     /gene="bepA"
                     /locus_tag="ACHKIR_003593"
     CDS             complement(12097..13560)
                     /gene="bepA"
                     /locus_tag="ACHKIR_003593"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HFO9991275.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            13773..14834
                     /gene="yfgO"
                     /locus_tag="ACHKIR_003594"
     CDS             13773..14834
                     /gene="yfgO"
                     /locus_tag="ACHKIR_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFO9991276.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQD"
     gene            complement(14933..15403)
                     /gene="bcp"
                     /locus_tag="ACHKIR_003595"
     CDS             complement(14933..15403)
                     /gene="bcp"
                     /locus_tag="ACHKIR_003595"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HFO9991277.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(15403..15975)
                     /gene="gcvR"
                     /locus_tag="ACHKIR_003596"
     CDS             complement(15403..15975)
                     /gene="gcvR"
                     /locus_tag="ACHKIR_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HFO9991278.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            16121..16999
                     /gene="dapA"
                     /locus_tag="ACHKIR_003597"
     CDS             16121..16999
                     /gene="dapA"
                     /locus_tag="ACHKIR_003597"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFO9991279.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASMKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            17016..18050
                     /gene="bamC"
                     /locus_tag="ACHKIR_003598"
     CDS             17016..18050
                     /gene="bamC"
                     /locus_tag="ACHKIR_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HFO9991280.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSEWHELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            18263..18976
                     /gene="purC"
                     /locus_tag="ACHKIR_003599"
     CDS             18263..18976
                     /gene="purC"
                     /locus_tag="ACHKIR_003599"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFO9991281.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            19144..20007
                     /gene="ypfJ"
                     /locus_tag="ACHKIR_003600"
     CDS             19144..20007
                     /gene="ypfJ"
                     /locus_tag="ACHKIR_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HFO9991282.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            20022..22037
                     /gene="tmcA"
                     /locus_tag="ACHKIR_003601"
     CDS             20022..22037
                     /gene="tmcA"
                     /locus_tag="ACHKIR_003601"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HFO9991283.1"
                     /translation="MAELTALHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTANNDAILWRQNQPFTL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPLGVAVVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATYVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFDDENFTHTPQGNIVISAFEQTLWRSEPQTPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENAIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGQQLIAGALQYTHDLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAKWNGETLPVD
                     PLNDIVLSADDWLELAGFAFAHRPLLTSLGCLMRLLQTSEMALPALRGRLQKNASDAQ
                     LCTTLKLSGRKLLLVRQREEAAQALYALDDVRTERLRDRITQWQFFH"
     gene            22111..22809
                     /gene="ypfH"
                     /locus_tag="ACHKIR_003602"
     CDS             22111..22809
                     /gene="ypfH"
                     /locus_tag="ACHKIR_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO9991284.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     TATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(22890..23090)
                     /gene="ypfN"
                     /locus_tag="ACHKIR_003603"
     CDS             complement(22890..23090)
                     /gene="ypfN"
                     /locus_tag="ACHKIR_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HFO9991285.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVNHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(23118..24245)
                     /gene="dapE"
                     /locus_tag="ACHKIR_003604"
     CDS             complement(23118..24245)
                     /gene="dapE"
                     /locus_tag="ACHKIR_003604"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HFO9991286.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(24249..24605)
                     /gene="yffB"
                     /locus_tag="ACHKIR_003605"
     CDS             complement(24249..24605)
                     /gene="yffB"
                     /locus_tag="ACHKIR_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HFO9991287.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAATLMTEMPAIIKRPLLCAPGKPM
                     LLGFSESSYQQFFHEV"
BASE COUNT         5813 a         6106 c         6469 g         6279 t
ORIGIN      
        1 tgcgggaaat aatttataaa aatacatcat cacaacatat gtattaatga tgtatttatt
       61 tcgcagtcga acaggataaa catcagtcag gacaaaaacg taaaatttaa gatgaaaaaa
      121 tatctgccgc tttgataatc ccaaagagca aaagcatccg acagcgaggt gccgttatgt
      181 tctgtgagag tggagaaggt aaccgctaac gttgtactgg tgattcgacg gagagtgtgg
      241 attgctgagc ttcttttttg ctctgatggt gctgccaggc accgacggac gagtagataa
      301 aacggccaaa gaagaagata aagctgatga gcagtacgat acgggtcatg cgactgttaa
      361 atcggtgtcg ttttcgcata ctggttgcct gactcacaaa aggttccttg aagtatgtcc
      421 cacgccgtgg acggtactta cattaaggca caacaggaca aaatggtcaa ttcttcgtta
      481 tgtaaaaaag cgtcagtgga tacatatttt aatgttatgg aagttaattt aattatttac
      541 aatgattatg taataatgat gagttaacat gataaacgta ataattcatt aatcagggtt
      601 attttgtttg atatatatca attggaactt tcatatgaca cttagaatca ttgctctcta
      661 tagtgatgaa taatcatcat tcgaagtcag gtgggatgtc tgtctgaata cacctccttc
      721 aggatgtggg ggattctgct gagcatctat gaaactgaat gcaacttata taaaaatacg
      781 tgataaatgg tgggggcttc cgctgttcct gccttcttta atcttgccca ttttcgccca
      841 cattaatact ttcgcgcata tttcttccgg tgaagttttt ctcttttatc tgccactggc
      901 actgatgatc agcatgatga tgtttttcag ctgggcggca ttgccaggga tcgccttagg
      961 gatttttgtc cgcaaatatg cagagctggg tttttacgaa acgctctcat taacggctaa
     1021 ttttattatc attatcattc tctgttgggg cggttacagg gtttttactc cccggcgtaa
     1081 caacgtttca catggtgata gccgtttaat ttcccagcga ctattctggc agattgtgtt
     1141 tcctgcaacg ctgtttctga tacttttcca gtttgctgcg tttgttggat tactggcgag
     1201 cagagaaaat ctggtcggcg tcatgccctt taacctcggg accttaatca attatcaggc
     1261 cttgctggtg ggtaatctga ttggtgtccc gctgtgctac ttcatcattc gggtggtgcg
     1321 aaatccgttt tatttacgta gctattattc gcaattaaaa cagcaggttg atgccaaagt
     1381 taccaaaaaa gagttcgcaa tctggctact ggcattaggt gctttactat tgctgttatg
     1441 catgccgtta aatgaaaaaa gcacgatttt tagcaccaat tacaccttgt cattattgct
     1501 gcccctgatg atgtggggag cgatgcgcta tggttataag ctgatttcat tgctctgggc
     1561 ggtcgtgttg atgatcagca tccacagcta tcaaaattac attcccattt atcctggcta
     1621 taccacgcag ctgaccataa cctcctccag ttatctggta ttctctttta ttgtcaatta
     1681 tatggctgta ctggcaaccc gtcagcgagc ggtagtcaga cgcattcagc ggcttgcgta
     1741 tgtggacccg gtggttcatc tgccaaatgt tcgcgccctg aatcgcgcgt tacgtgatgc
     1801 cccctggtct gcgctttgtt atttacgcat ccctggcatg gaaatgctgg ttaagaatta
     1861 tggcatcatg ctgcggattc aatacaagca aaaactttct cactggctgt caccattgct
     1921 ggaaccgggt gaagatgttt atcagctttc gggtaacgat ctcgcgctgc ggctgaatac
     1981 agaatcgcac caggagcgca ttaccgcact ggatagccat ctcaagcaat ttcgtttctt
     2041 ttgggatgga atgccgatgc aaccgcagat tggcgtcagt tactgctatg tgcgctcgcc
     2101 agtgaatcat atctacctgc tgctgggaga gctaaataca gtggccgaac tttctatcgt
     2161 gaccaacgcc ccggaaaata tgcagcgtcg cggagcaatg tatttgcaac gcgaattgaa
     2221 agataaagtc gcgatgatga atcggctaca gcgggcgctg gaacacaacc attttttcct
     2281 gatggcccag ccgattaccg gtatgcgtgg tgatgtctac catgaaattc ttctgcgcat
     2341 gaaaggtgag aatgatgaac tgatcagccc cgatagcttc ttaccggtcg cgcacgaatt
     2401 tggtttatcg tcgagtatcg acatgtgggt cattgagcat acgctgcaat ttatggctga
     2461 aaacagagcg aagatgcccg ctcaccgttt tgcgattaat ctgtcgccaa cctcggtatg
     2521 tcaggctcgt tttcctgttg aaatcagtca gctgctggct aaatatcaga ttgaagcgtg
     2581 gcaacttatt tttgaagtca ccgaaagtaa tgctctgacc aatgttaagc aggcgcaaat
     2641 caccttgcag catcttcagg aattaggctg ccagattgcg attgatgatt tcggcaccgg
     2701 ctacgccagc tatgcgcggc ttaaaaatgt gaatgccgat ctgcttaaaa ttgacggcag
     2761 ttttatccgc aatattgtgt caaatagtct ggattatcag atagtggcgt cgatttgcca
     2821 cctggcgcga atgaagaaaa tgcgggtagt ggcagagtac gttgaaaacg aagagatccg
     2881 cgaggcggtg ctctctttgg ggatcgatta tatgcagggt tatcttattg gtaagccgca
     2941 accgttaatt gatacgctga atgaaatcga acccattcgc gaaagtgcct gaataatgcg
     3001 ggccgacatt tctcgtcggc ccgcaaagca ttaagcggcg atttctggtg tactttcttc
     3061 ttcaattttc aaccgccagc cagccacgcc ttcccagtat tcttgctcct tttccagatc
     3121 aagcagtacc agcgcattct gactaaacca gtcatgcgga aaacgcagtg tccagtgact
     3181 gtcatcggta atcagcgtca atgttggcgg tgtggtggtt gcctgacgtt gattattgag
     3241 taatacgcca aggcgcaata gctgaatcag tggcaggaac tgtttcttct taaacaaggt
     3301 aaagcgcggc agatcgtcga gcttaatcgc tttacggtga tagcgcacca gcgtcgccat
     3361 catcaactgc tgttcctgat taaaacccgg caagtcgctg ttttgcagaa tataagcgga
     3421 gtggcgatgc aaaccgctgt ggttgatatt caacccgacc tcatgcagca tggcggccca
     3481 tcgcagtagc gcttccagtt gcggatgcgc cagcttcggt tgctgttccc gccactgttc
     3541 gtacatttgc atagtggtat ccagcacccg tcgggcctgt tcgctgtcga tgtgatactg
     3601 gttggcgagg ctgctggcgg tgcgactacg cacatcctga tgacggaaac gtccttccat
     3661 ttcatacagt acgccttcgc gtaacgcccc gtcagaaagg cgcagttcac ggatggctaa
     3721 agcatcaaac acaccgcata aaatcgccag tccaggaacg aagactgttt tccgttcttc
     3781 ggaaagacct ggtaagctca gcgatgcgaa attacggtgc cgtaaaactt cttttaccag
     3841 tttttccaga cgttccgggg taattatccc gtctttctcg cccatttcca ttaacacttc
     3901 atgggcggct tttattgtac cggaagcgcc cattgcaaca ttccagccct gaattcgaaa
     3961 ttgccaggtt aaagtttcca gtttttgtgc tgccgccatg cgagcgcgct gaaaattctc
     4021 tttattgatg accccgccag ggaaataaag ctgggcaaag ctgacacaac ccatccggcg
     4081 gctttcaacg agaatcggtt cgaaattttc accaatcacc agttccgtag atccgccgcc
     4141 aatatcaata accagtttgc gacctttctc cggttgggta tgttccacgc ccataaaaat
     4201 cagacgggct tcttcattac cggaaataat ttcaatcggg tagggaatga ccttttccgc
     4261 gcgtttcaga aagtcagtgg cgttcagcgc ctgacgcagt gtatgggtgc caactataca
     4321 gacgctggca ggagaaaacc cttgcagccg ttcggcaaac agcgacagac agtttaaacc
     4381 gcgcgtcatt gcctcttcac tcaacatatt atctggcccc aggccgtccg ccagatgcac
     4441 tcgctgtttc aggcggccaa taatctgcat ggcaccatct accacacggg ctatgaccat
     4501 gtgaaaactg tttgaaccaa gatcgaccgc agcaaactcc tgcggacgag gggatttatc
     4561 gtgtattggc atagggttat tcaggttgtt cgagtgattt gatgtagtcg taaatcgcca
     4621 actgcgcccg tactttacgg cgattgccgc ggggaacgta gcgattactg agttctttat
     4681 cgatataacg tgctttgacc gtatcgctga acaatatgtc gatgatgtcc agcacccgct
     4741 gcttcaggcg cggatcgagc agcggtgtcg ccacttcaat acgataatca atattgcgcg
     4801 tcatccagtc ggcggaagaa aggtagacct ttttatcgcc gccattttca aaaatataaa
     4861 cccggtcatg ttcaaggtaa cggtcaacaa tactgatggc acgaatgttg tcgctaatgc
     4921 cttccagatt ggggatcagc gaacacatgc cgcgaaccag cagattaacc ggtacgccgg
     4981 agctggaggc cgcatacaga cgatcaacca ggcctttatc gacaaggtta tttagcttca
     5041 gggtgatacc gctgggcagc ccttgctgcg cgttggcgat ttcgcggtcc accatttcat
     5101 acaacaggcg gcgggagttt tgcggcgaca ccattaaata atcaaatgtc accggacggt
     5161 atgggttttc aataaagttg aacacccgcc gtacttcgtt ggttatgcgc gcatcggcgg
     5221 tcagcaacga atagtcagta taaagacgcg cggttttttc gttaaagttc ccggtcccga
     5281 tgtgtgcgta acgcaccact tcaccgtttt ctttacgtga aatcaggaac agtttggcgt
     5341 gaattttcag ccccggcgca gagaagataa cgtgcacgcc tgcttcggtc aggcgcttcg
     5401 cccagtgaat gttggcttct tcgtcgaaac gcgcctgtaa ctcaaccacc acggtgactt
     5461 tcttaccgtt gtgtgcggcg tggatcatcg agtcgatgat gcgtgaatct ttcgccacgc
     5521 ggtaaatgtt aattttaatc gccagtacgc tcgggtcgaa cgaagcctga cgcagcagtt
     5581 ccagtacatg ctcaaaggta tgataaggat aatagagcaa cacatcgcgt tcgcgaatag
     5641 catcaaaacc attgcggaac tgggctttat caaaccaaat gtggcgtaaa cgcggcagtg
     5701 gtttgttcac cagattggct ttgccgacat tggggaaatt aataaagtct ttaaaattat
     5761 gataacgacc accggggacg atggagtcgt agcgggaaat agtcagtttt tcgcgtaaca
     5821 cttcaaccag cgcattgggc atatcgcgct gataaacaaa acgcaccggc tcagcagtta
     5881 aacgctgctt gagactggaa gacatcaact ccatcaggct ggcttccatc tcatgcacta
     5941 aatcgtattc ggcatcgcgg gtcatcttca ttgaataggc attcagcgcg tcataatcaa
     6001 agaagccttt gaaaatatca tcgaggcagt aacgcagaat gttatccaga agaatcatcg
     6061 gcttgcgtcg acgtggcgct tctggcggta aattcacaaa gcgcggcact ttatctgatg
     6121 ggatctccag cagcgcgtaa cggatggtat cgccacggat aatttccacc gccagatagg
     6181 tgtaatcatc tttcaggaac tgcactaagt cagtgtcagg attgattaaa atcggcgtaa
     6241 tgtgctgacg cagatactgc ttaaaataat gacgcagcca gttttgttga ttgacggaga
     6301 gctggcgttc attaatcagg aagatctggt tgcgcgccat ctccagcaac agctcgttgt
     6361 agaggccgtc gaattcctga tcggctttca gcacccggga ctgaattttg cccagtaaat
     6421 ggcgggaatg agagttggaa ccttgttctt cgctaataat gatgcgtcgc ttcagttcag
     6481 cgaagcggac tttatagaac tcatcaaggt tattggaata gatccccagg aaacgcatcc
     6541 tttcaatcag cgggttagat ttgtccgccg cttcctgaag cacgcgttca ttgaacgata
     6601 accaactgag ctctttttcg atgtatagct tttcctgacc cattaccact tttactccgt
     6661 tttattcacg ggacactgcc tggatattat ggcgagcaat attggcagat gtccaactat
     6721 gccagaaaag ggggaaaaaa actgctggag cttgcgattt acaggtgaat tgaaaaacgc
     6781 cagcggcaga gctggcgctt taactacggg gcattactcg tcggcagcgt agccctgcgg
     6841 cggcagacgt tgaccatcca gccacgcggc gttttcgtgc attttcagac gaccatcggc
     6901 aaaccagcta atcaccagtg gataaatagc gtgttcctgg gtttgcacgc gggcggtgat
     6961 gtcatcttcc gtatcaccag caaataccgg gactttcgcc tgtaaaataa ccgggccacc
     7021 gtccagttca tcggtgacga aatgcaccga tgtaccgtgc tcttcatcgc cattttccag
     7081 cgcctgacga tgggtgtgta atccgggata tttcggcagc agagaagggt gaatgttcag
     7141 caaacgcccg gcatagtggg agacaaacgc cgggctgaga atgcgcataa aaccagccag
     7201 cacgaccaca tcgggtgcgt acatgtcgat ttcatgaatc aactcccggt cataggcttc
     7261 acgactgtca aacgcgctgg cgatgagcgt atgcgttgca atacccgcct ggcgggcgcg
     7321 ttcaaggccg aacgcgtcgg ccttattgct gaaaactgcc cgtacggtgc ctttaatttt
     7381 gttggttttg caggcgtcaa taattgcctg caaattactt ccgttgccgg aaataagcac
     7441 cacaatattc attattcgat aaccacgcgt tgttcggaat cagaggcttt gatgataccg
     7501 attttccacg cgttttcacc gttggcattg agcagggcga gagctttgtc cacttccgga
     7561 gcaggcaggg caataatcat accgacgccg cagttgaagg tgcgatacat ttcatggcgc
     7621 tcaacattac ctgccgtttg cagccagttg aacacttccg gccactgcca ggaagattca
     7681 tcaatcactg cctgggtatt atctggcaat acgcgcggaa tgttttccca gaagccgccg
     7741 ccggtcaggt gcgcaatggc atgcacatcg accttttcaa tcaatttcag cactgacttc
     7801 acgtaaatgc gggtcggtgc cagcagatga tcggctaatg gcttaccatc aagttcggtg
     7861 gtttgcggat cacaaccgct gacttcaaga attttgcgca ccagcgaata gccattcgag
     7921 tgcgggccgc tggagccgag tgcaatcagc acatcgccgt cgctgacttt agatccgtcg
     7981 atgatttctg atttttctac cacaccaacg cagaaaccag cgacatcgta atcttcaccg
     8041 tgatacatcc ccggcatttc cgccgtttcg ccacccacca gtgaacagcc cgattgcaga
     8101 caaccttccg caatgccgct gatcaccgct gaagcggtat caacatccag ttttccggtt
     8161 gcgtaatagt cgaggaaaaa cagcggttcc gcaccttgca ccaccaggtc attaacgcac
     8221 atggcgacca gatcaatacc aatggtgtcg tgacgtttta agtccatcgc cagacgcagc
     8281 ttggtaccta cgccgtcagt gccggaaacc agcacaggtt cacgatattt ttgcggcaat
     8341 gcacacagcg caccgaagcc gcccagaccg cccatcactt ccggacgacg cgttttcttc
     8401 actacgcctt tgattcttcc aaccagagca ttacccgcgt caatatcaac accggcatct
     8461 ttgtagctaa gagaggtttt atcggtcact gcttgggtcc ccacgcgtta cttgcggtag
     8521 aaaaataaaa ttcggcgcaa ttctaacagg gaaagcaaac gtttgcgaga ctgctttaca
     8581 caaccttttt gcacgtcttt tccccaggcg cgcggcgaaa gaatacttgt actagggcaa
     8641 aggatcgttt tcggatggaa taatcttctt ccataaccat ttgaatataa aataacttta
     8701 tctcaaaccg ttatcatttt gactaaagtc aacgaaaata atattgccgc cttgaagaaa
     8761 ggaggtataa tccgtcgatt ttttttgtgg ctgcccctca aaggagaaag agtatgaaga
     8821 tcgtggaagt caaacaccca ctcgtcaaac acaagctggg actgatgcgt gagcaagata
     8881 tcagcaccaa gcgctttcgc gaactcgctt ccgaagtggg tagcctgctg acttacgaag
     8941 cgaccgccga cctcgaaacg gaaaaagtca ctatcgaagg ctggaacggc ccggtagaaa
     9001 tcgaccagat caaaggtaag aaaattaccg ttgtgccaat tctgcgtgcg ggtcttggga
     9061 tgatggacgg tgtgctggaa aacgttccga gtgcgcgcat cagtgtcgtc ggtatgtacc
     9121 gtaatgaaga aacgctggag ccggtaccgt acttccagaa actggtttct aacatcgatg
     9181 agcgtatggc gctgatcgtt gacccaatgc tggcaaccgg tggttccgtt atcgcgacca
     9241 tcgacctgct gaaaaaagcg ggctgcagca gcatcaaagt tctggtgctg gtcgctgcgc
     9301 cagaaggtat cgctgcgctg gaaaaagcgc acccggacgt cgaactgtat actgcatcga
     9361 ttgaccaggg actgaacgag cacggataca ttattccggg cctcggcgat gccggtgaca
     9421 aaatctttgg tacgaaataa agaataaaaa taattaaagc cgactttaag agtcggcttt
     9481 tttttgagta aagcgcctat aacacataat acagaggata atactatgac gcgccgtgct
     9541 atcggggtga gtgaaagacc gccactttta cagacaatcc cgcttagttt gcaacatttg
     9601 ttcgccatgt ttggtgcaac cgtcctggtg cccgtcttat ttcatattaa cccggcgact
     9661 gtactgttat ttaacggtat aggaacactg ctgtatctct tcatctgtaa agggaaaatt
     9721 ccggcttatc ttggttccag ctttgccttt atttcaccgg tattgttact gttgccgttg
     9781 gggtatgaag ttgcgctggg cggctttatt atgtgcggcg tgctgttctg cctggtttct
     9841 tttatcgtga agaaagcggg gaccggctgg ctggacgtgc tgtttccacc tgcggcaatg
     9901 ggcgcaatcg ttgccgtcat cggtctggag ctggcgggcg tagctgccgg tatggcgggt
     9961 ttactcccgg ctgaagggca aacgccagac tccaaaacca tcatcatctc tattaccacc
    10021 ctggcggtca cggttttagg ttccgtgctg tttcgtggtt tcctggcaat tatcccgatt
    10081 ttaattggcg tgctggtggg gtacgcgctc tctttcgcaa tgggaattgt cgataccacg
    10141 ccgattatta atgctcactg gtttgcgctg ccgaccctct atacgccgcg cttcgagtgg
    10201 tttgccattc tgactattct accagcggcg ttagtggtta ttgccgaaca cgtagggcac
    10261 ctggtcgtaa cggctaatat cgtcaaaaaa gatctgctgc gcgatccagg tctgcaccgt
    10321 tcgatgtttg ctaacggctt gtcgaccgtg atctccggct tctttggctc cacgccaaac
    10381 accacttacg gagaaaacat tggcgtgatg gcgatcaccc gtgtttacag tacctgggtt
    10441 atcggcggtg cggcgatttt cgctatcctg ctttcctgcg tcggtaaact ggctgcagct
    10501 atccagatga ttccattgcc ggtgatgggc ggcgtttcgc tgctgcttta cggtgtcatc
    10561 ggtgcttccg gtattcgtgt gttgatcgaa tcgaaagtgg attacaacaa agcacagaac
    10621 ctgatcctga cttccgttat tttgatcatc ggcgtcagtg gcgcgaaggt aaacattggc
    10681 gcggcggagc tgaaaggtat ggcgctggcg accatcgtcg gtatcggctt aagtttgatc
    10741 ttcaaactga ttagtatgct gcgccctgaa gaagtggtgc tggacgcaga agacgcggat
    10801 ataacagaca aataatgtac cgaccgggca gtgttgctgc ccggttcaaa catcatggga
    10861 ttctgtggta aacttctcgc gattttgtga aatcctggtt gaggtatctc tgaacacacc
    10921 ggcacagctc tctttgccac tttatcttcc tgacgacgaa acctttgcaa gtttctggcc
    10981 gggggataac tcctctttac tggccgcgct gcaaaacgtg ctgcgtcagg aacatagcgg
    11041 ttacatctat ctctgggcac gcgaaggcgc ggggcgcagc catctgctgc acgcggcttg
    11101 cgcggaattg tcgcagcgtg gcgatgcggt gggctatgtc ccgctggata aacgcacctg
    11161 gtttgttccg gaagtgctcg acggtatgga gcatttgtcg ctggtctgta tcgacaacat
    11221 tgagtgtatt gcaggcgatg agttgtggga gatggcgatt ttcgatctct acaatcgaat
    11281 tctggaatcg ggcaaaacac ggttgttaat caccggcgat cgtccaccgc ggcagttgaa
    11341 tcttggatta ccggatctcg cgtcgcgtct cgactggggg cagatctaca aattgcagcc
    11401 actttctgat gaagataagt tgcaggcgct acagttacgc gcgcgtttgc gtggttttga
    11461 actgccggaa gatgtggggc gtttcttgct gaagcggctc gacagagaga tgcgcacgtt
    11521 atttatgacg ttggatcagt tggatcgtgc gtcgattacc gcgcaacgta agctgaccat
    11581 tccgtttgtg aaagaaattc tgaaactgta gacaagacgt ttattgtcgg atatgcaccc
    11641 atagtattcg tcggccggat aagacgcggc aagcgtcgca tccggcaatg tgcttcaact
    11701 agcgatggag cacttcttaa cccacgattt ccagtacctg ctccggtgga cgaccaatcc
    11761 gcgctttgcc attcgcgacc acaatcgggc gctccattag cttcggatta tccaccattg
    11821 cctgaatcag cgcctcttcg ctaagcgaac tgtcagccag gttcagttct ttataaagat
    11881 cctctttctg gcgcatcaat tcacgagcgc tattcatccc cagcattttc agcaaatcac
    11941 gcagcgttgc cgcatcggcg ggtgtctcaa ggtagagcac cacttctggc tccacgccgt
    12001 tttctttcag caggttcagc gtttcccggc tctttgaaca gcgtgggtta tggtagattt
    12061 ttacctgttt ggtcattgtt cttcctttaa tgcgaattac atcttggtat aaggcttaaa
    12121 gcgttcctgc agctggcgca actggtcgat gcgcgcatcg taacgcgctt gttgcaggct
    12181 gcctaatttc acctgcgaac tggcgctact caacagcgaa atggcctgat cgagtcgtcc
    12241 ggcgagcgca taaccttctg ctcgcgcagc cagctcctga tcgcggttat ttagcgcggc
    12301 ttccgcctgt gccagcaaat cccagccgtt gctgtcatct ttattattaa aggtgtagcg
    12361 attcagaata ttggccgctt cttgtggttg accgccttgc agataagcgt tcgccaggtt
    12421 gagctgcaac accggattgg tgcgtaaatc gcgggcattt ttcagacgat tgatcgcctc
    12481 attggctttg ttttgcccaa gatcgatatc agtagccaga tcgagatacc atgcgttgcc
    12541 aggttctgcc gccagtaacg gttgcagcgt tttacgcgcc tcgtcgtatt tattggcttc
    12601 catcgcctgt aaagcacgac catattgcgc cgctcgttgc tgacgaacgt ttcctttcgc
    12661 ccattcatcc agcaaatcac tggtgagctg gttacgtccg gaattataca tccccagtgt
    12721 gcgcgctttc gccagataga aatcttccga cgactgcacc accatcgggc gcatctgatt
    12781 agcacggttg cgggcatctg ccagacgact ttccggcaac gggtgagtca ataaaatttc
    12841 cggcggacgc gaggagtaac gcgcctgatc gagtaatttt tcgaggaagg tcggcatcgc
    12901 ctgcggatcg aatcctgagc gttgcagcac ctgaatacca atgcggtccg cttcctgttc
    12961 attttgctgg gtgaagctga tcatcccctg gcgcgttccc gccagtgtac cggtcagcgc
    13021 agccatcccc gcctgcggac tggccatcgc cagtaaaata gaacctaacg cgccgaccca
    13081 ggtcagcggc gcgctgcgct gctgatcttc catcgctcgc gccaggtgac gttgggtgac
    13141 gtgggagatt tcgtgcgcca taactgaagc cagttgactt tcgttatcgg aataacggaa
    13201 cagggcagag tgcagcacca cgttgccgcc aaagaaagca aaggcgttaa tttcatcgtt
    13261 gttgatcaga aagaaatgaa acggtgtctt aaccgaattg gcatgcgaaa ccagacgcat
    13321 tcctagcgaa ttaatgtatt gcgttaacag cggatcatta attaacggcg cgctgccgcg
    13381 tagctggcgg acataatagt cgcccatctg catttcctga ccaatggaaa gtgtgcttcc
    13441 tgcggaggtt cccatatccg gcaaggtgtc tgcgctgtcg gcaaacgccg gggctacctg
    13501 accaatggtc atagcagcaa tgagggttgc aaccaggttt tttttcaact gcctgaacat
    13561 aacctctatc ctgtatttct ggagatagtc atttgaccga tactgtgaaa tatcgttccc
    13621 accgcgccgc ctttgagtgt aacggcgaag attagcccac tcaaatgact tttcagtttc
    13681 ctgaatccgt cataaaaagc gaagtctttt ttgtggcatt tcgatacaat tgcggatcgc
    13741 aatcccgctc ttgaggttca gggaaggtct gtatgctcga aatgttgatg caatggtatc
    13801 gccgccgttt tagcgacccg gaagcgattg ccttgctggt tattttagtt gccggatttg
    13861 gcattatctt tttctttagt ggcctgcttg ctccgctgtt ggtggctatt gtgctggcct
    13921 atttgctgga atggccaacc gtgcgcctgc aatctattgg ctgctcccgc cgctgggcga
    13981 cgtcgattgt attggtggtt ttcgtcggta tattgctact gatggcgttc gtggtactgc
    14041 ctatcgcctg gcagcagggc atctacttaa tccgcgatat gccggggatg ctcaataagc
    14101 tttctgactt tgccgccacg ttgccgcgcc gctatccggc gttaatggat gcgggcatta
    14161 ttgatgcaat ggccgaaaat atgcgcagtc ggatgctgac catgggcgat tcggtggtga
    14221 aaatttccct cgcctcgctg gtcggtttgc tgaccattgc cgtctatctg gtgctggtac
    14281 cattgatggt cttcttcctg ctgaaagaca aagagcagat gctgaacgcc gttcgccggg
    14341 tgctgccgcg taaccgtgga ctggcaggac aggtgtggaa ggagatgaat caacaaatca
    14401 ccaactatat ccgcggcaaa gtgctggaga tgatcgtggt ggggattgcc acctggctgg
    14461 gattcttact cttcgggctg aactattcgc tgctgctggc ggtgctggtc ggcttctcgg
    14521 tacttattcc gtacattggt gcatttgtgg tgaccattcc ggtggttggc gtggcgctat
    14581 tccagtttgg tgctggcacg gaattctgga gctgcttcgc ggtgtatctg atcattcagg
    14641 cgctggacgg caacctgtta gtacctgtgt tgttctccga agcggttaac ctgcatccgc
    14701 tggtcattat tttatcagta gtgatcttcg gtggtttgtg gggattctgg ggcgtattct
    14761 tcgccattcc attggcgacg ctgatcaaag ccgtgattca cgcctggccc gatgggcaaa
    14821 tcgcgcaaga ctgaccttca gcgaaaaaat ttgaaacgag tgttgcaggg cagtgagaag
    14881 atgtgagatg tgctggccgc aagcgcagcc agcacggaat ggagcaaagt aatcaggcgt
    14941 gttctttcag ccagttcagc acaacgtcgt ggtgattgct ggttttgaaa tcgtcaaaga
    15001 catgttcgat tttgccatca gcgtcaatca ggaagctgat gcgatgaata ccatcgtagg
    15061 ttttgcccat gaaggacttt tcaccccaga cgccgaattg ttcgcacacc tggtggtcct
    15121 catcagacag gagcgtaaag ttaagcagct ctttttccgc aaaacgggag agtttttcgg
    15181 gtttatcggt gctgataccc agcacatcaa cgcccgcttt tttcaactca tccatgttat
    15241 cgcgtaagcc gcaggcctgt acggtacagc cgggggtcat ggctttcggg tagaaataaa
    15301 ccagaacacg ctgtccctgg aagtcggtca aattaacttg ttctccgtct tgatccggca
    15361 agctaaattt cggtgcgata tcaccggctt tcagtggatt cattacttaa ctccatcctg
    15421 ttcatcatgt tgggaataat tgacgacgtt aatactgcct tgtgcattga gttctgtaca
    15481 tagggctttg aatgcttgct caatatttgc tgcgtccgca gatgcggggc tgtgggcggt
    15541 tatctgaata tgcaactgcg cagcccgttc attttcagca ggttgcgtgc gcgacaccag
    15601 ctccgcaatg ttcatatgat gcgcgtcgaa aagtgctgtg aagcgttcaa ttaaatgcgg
    15661 ggagtctgcc acatcgacct gaacccagac agatgctggc attggcggac gcggacgcgc
    15721 cgtcgtgcgc ttcatcacga ttaaaagatc cagctcggca cctttcaacg gtaacgttga
    15781 ttcaatcaga gtaatggcat tccatgaacc ggaaagcagc atgataaacg tgaactcttc
    15841 tcccagcatc gccaggcgac tgtcttcaat attacagccg caactactga catgacgggt
    15901 gatggtgttc acaattccag ggcgatcggc acccaacgca gtgatcacca gataatgttg
    15961 cgatgacagt gtcaaactgg ttattccttc aaggggtgag ttgttcttaa ggaaagcata
    16021 aaaaaaacat gcatacaaca atcagaacgg ttctgtctgc ttgcttttaa tgccatacca
    16081 aacgtaccat tgagacactt gtttgcacag aggatggccc atgttcacgg gaagtattgt
    16141 cgcgattgtt actccgatgg atgaaaaagg taatgtctgt cgggctagca tgaaaaaact
    16201 gattgattat catgtcgcca gcggtacttc ggcgatcgtt tctgttggca ccactggcga
    16261 gtccgctacc ttaaatcatg acgaacatgc tgatgtggtg atgatgacgc tggagctggc
    16321 tgacgggcgc attccggtga ttgccgggac cggtgctaac gctactgcgg aagccattag
    16381 cctgacgcag cgcttcaatg acagcggtat cgtcggctgc ctgacggtaa ccccttacta
    16441 caatcgtccg tcgcaagaag gtttgtatca gcatttcaaa gccatcgctg aacatactga
    16501 cctgccgcaa attctgtata atgtgccgtc ccgtactggc tgcgatctgc tcccggaaac
    16561 ggtgggccgt ctggcgaaag taaaaaatat tatcggaatc aaagaggcaa cagggaactt
    16621 aacgcgtgta aaccagatca aagagctggt ttcagatgat tttgttctgc tgagcggcga
    16681 tgatgcgagc gcgctggact tcatgcaatt aggcggtcat ggggttattt ccgttacggc
    16741 taacgtcgca gcgcgtgata tggcccagat gtgcaaactg gcagcagaag ggcattttgc
    16801 cgaggcacgc gttattaatc agcgtctgat gccattacac aacaaactat ttgtcgaacc
    16861 caatccaatc ccggtgaaat gggcatgtaa ggaactgggt cttgtggcga ccgatacgct
    16921 gcgcctgcca atgacaccaa tcaccgacag tggtcgtgag acggtcagag cggcgcttaa
    16981 gcatgccggt ttgctgtaaa gtttagggag atttgatggc ttactctgtt caaaagtcgc
    17041 gcctggcaaa ggttgcgggt gtttcgcttg ttttattact cgctgcctgt agttctgact
    17101 cacgctataa gcgtcaggtc agtggtgatg aagcctacct ggaagcggca ccgcttgcgg
    17161 agcttcatgc cccggctgga atgattttgc cggtgacctc cggcgattat gcaatcccgg
    17221 tgaccaacgg tagtggtgct gtcggtaagg cgctggacat tcgtccacca gcccagccgt
    17281 tggcactggt ttctggcgcg cgtacccagt tcacgggcga taccgcttca ttgctggtgg
    17341 aaaatggtcg tggcaatact ctgtggccgc aggtggttag cgtgctgcag gcgaaaaact
    17401 acaccatcac ccaacgtgat gatgctggtc agacactgac caccgattgg gtacaatgga
    17461 accgtctgga cgaagacgag cagtatcgtg gtcgttatca aatctctgtt aagccgcagg
    17521 gttatcagca ggcggttacg gttaaactgc tgaacctgga acaggcgggc aaaccggttg
    17581 cagacgcggc ttccatgcag cgttacagca cggagatgat gaacgttatt tccgccggtc
    17641 tggataaatc tgccactgac gccgcgaacg ctgcgcaaaa tcgtgcctcc accactatgg
    17701 acgtacaaag tgcagctgat gacaccggtt taccaatgct ggtcgtacgc gggccgttca
    17761 atgtggtttg gcaacgtctg ccagctgcgc tggaaaaagt gggcatgaaa gtgaccgaca
    17821 gcacccgttc gcagggcaac atggccgtaa cttacaagcc gctgtctgac agcgaatggc
    17881 acgaactggg cgcgagcgat ccaggtctgg catccggtga ctataaactg caggttggcg
    17941 atttagataa ccgcagcagc ctacagttca tcgatccgaa aggtcatact ctgactcaga
    18001 gtcagaacga cgcgctggta gctgtcttcc aggctgcgtt tagcaagtaa aaatacaggg
    18061 ctggagtcat ccggcccttt tttctgatat gatacgcaaa cgtgtgcgtc ggcagaaaaa
    18121 cgcgatttta gcggtaattc gcacgaaatt tgtttgtcgg acgtaggtcg gataaggcgt
    18181 tcacgccgca tccgacaaaa catccggcac accagacagc aatagatttt aaaacgttaa
    18241 ttcacaccca ggagtgataa agatgcaaaa gcaagctgag ttgtatcgtg gtaaagcgaa
    18301 aaccgtatac agcacggaaa acccggacct gttggtgctc gaattccgca atgatacgtc
    18361 agcaggggat ggcgcgcgca ttgagcagtt tgatcgcaaa ggtatggtga acaacaagtt
    18421 caactacttc attatgagca aactggctga agcgggtatc ccgactcaaa tggagcgtct
    18481 gctctccgat accgaatgtc tggtgaaaaa gctggatatg gtgccggttg agtgtgtcgt
    18541 gcgtaaccgt gctgctggct ctctggtgaa acgtcttgga atcgaagaag gtattgagct
    18601 gaacccgcca ctgttcgatc tgttcctgaa aaacgacgcc atgcacgatc cgatggtcaa
    18661 cgaatcttac tgcgaaacct ttggctgggt aagcaaagag aacctggcac gcatgaaaga
    18721 gctgacctac aaagcgaacg acgtgctgaa aaaactgttc gatgatgctg gtctcattct
    18781 ggtcgacttc aagctggagt ttggtctgta caaaggtgaa gtggtactgg gcgatgagtt
    18841 ctccccggac ggcagccgct tgtgggacaa agaaacgctg gagaaaatgg acaaagaccg
    18901 tttccgccag agcctcggtg gcctgatcga ggcctatgaa gccgtcgccc gccgcctggg
    18961 tgtacagctg gactgatttt tctgttcatc atcttgccgt gcagctggca cggcaagaca
    19021 accgctctca taagatgagt attgaacgct cattcctccg ccatttcatc ccgctttcct
    19081 gtggtaatct tcctctgaac cacctacgat catttctata atgaatatat tgttgaggta
    19141 tctatgcgtt ggcaagggcg acgtgaaagt gacaatgttg aagacaggcg caacagctct
    19201 ggtggtccgt caatgggcgg tccaggtttt cgcctgccaa gcggtaaagg cgggctgatt
    19261 ttactgatag tcgtgctggt tgcaggctac tatggcgttg atttaaccgg gttgatgacc
    19321 ggacagccgg tttcccaaca gcaatcaaca cggtcaatta gtcctaatga agacgaagcc
    19381 gcaaaattca cttcggtgat tctggcaacc acggaagaca cctggggaca gcaattcgag
    19441 aagatgggta aaacctatca gcaaccgaag ctggtcatgt accgtggaat gacgcgcacc
    19501 ggctgcgggg cgggccagtc cataatggga ccgttctatt gcccggcgga tggcacggtt
    19561 tatatcgatc tctccttcta tgatgacatg aaagacaaac ttggcgcgga tggcgatttt
    19621 gcccaggggt acgttatcgc ccatgaagtc ggtcatcatg tgcagaaact gttaggcatc
    19681 gagccgaaag ttcgtcaact gcaacaaaac gcgacgcagg cggaagtgaa ccgcttatct
    19741 gtgcgtatgg aactccaggc cgactgtttt gccggtgtct gggggcatag tatgcagcag
    19801 caaggtgttc tggaaaccgg cgatctggaa gaggcgctga acgcggcgca ggccatcggc
    19861 gatgaccgtt tacaacagca aagtcagggg cgagtagtac cagacagttt cactcatggc
    19921 acttctcagc aacgctacag ctggtttaaa cgtggtttcg acagcggcga tccggcacaa
    19981 tgcaatactt ttggtaaaag catttaactt ccggggcagg gatggctgaa ctgactgcgc
    20041 ttcacacatt aacagcgcaa atgaaacgtg aagggatccg ccgcttgctg gtgttgagcg
    20101 gggaagaggg ttggtgtttt gatcatgcgc ttaagttgcg tgatgcctta cctggcgact
    20161 ggctgtggat ttcgccgcag ccagatgctg aaaaccactg ttctccctcg gcgctacaaa
    20221 ctttacttgg gcgcgagttc cggcatgcgg tattcgacgc ccgccacggc tttgatgccg
    20281 ctgcctttgc ggcacttagc ggaacgttga aagcgggaag ctggctggtt ttgttactcc
    20341 ctgtatggga agagtgggaa aaccaacctg atgccgactc gctgcgatgg agtgattgcc
    20401 ctgaccctat tgcgacgccg cattttgtcc agcatctcaa acgcgtactt acggcgaata
    20461 acgacgctat cctctggcgg cagaaccagc cgttcacgtt ggcgcatttt actccccgta
    20521 ctgactggca ccccgcgacc ggcgcaccac agccagaaca acagcaactc ttacagcagc
    20581 tactgaccat gccattgggc gtggcggtgg taacggctgc gcgtgggcgc ggtaaatcgg
    20641 cgctggcagg gcaactcatt tctcgtattg cgggtagtgc gattgtcacc gcgcccgcaa
    20701 aagcggcaac gtatgtactg gcacaatttg cgggcgagaa gtttcgcttt attgcgccgg
    20761 atgccttgtt agccagcgat gagcaagccg actggctggt ggtcgatgaa gccgcagcca
    20821 tacctgcgcc gttgttgcat caactggtat cgcgttttcc tcgaacgttg ttaaccacta
    20881 cggtgcaggg ctacgaaggc acgggacgtg gttttttgct gaaattttgc gctcgctttc
    20941 cgcatttaca ccgttttgaa ctgcaacagc cgatccgctg ggcacaggga tgcccgctgg
    21001 aaaaaatggt tagtgaggca ctggtttttg acgatgaaaa cttcacccac acaccacaag
    21061 gcaatatcgt catttccgca tttgaacaga cgttatggcg aagcgagcca caaacgccgt
    21121 taaaggttta tcaattattg tctggtgcgc actaccggac ttcgccgctg gatttacgcc
    21181 gcatgatgga tgcaccaggg caacattttt tacaggcggc tggcgaaaac gcgattgccg
    21241 gggcgctgtg gctggtggat gagggcggat tatctcaaga actcagtcag gcggtatggg
    21301 caggttttcg tcgcccgcgg ggtaatctgg tggcccagtc gctggcggcg cacggcagca
    21361 atccactggc ggcgacattg cgcggaaggc gggttagccg gatagcagtt catccggctc
    21421 gtcagcggga aggcacaggt cagcaactga ttgctggtgc tttgcaatat acgcatgacc
    21481 tcgactatct ttcggtgagt tttggttaca ccggggaatt atggcgtttc tggcaacgct
    21541 gcggttttgt gctggtgcga atgggtaatc atcgtgaagc cagcagcggt tgctatacgg
    21601 cgatggcgct gttaccgatg agtgatgcgg gtaaacagct ggctgaacgt gagcattacc
    21661 gtttacgtcg cgatgcgcaa gctctcgcaa agtggaatgg cgaaacgctc cctgttgatc
    21721 cactaaacga tatcgtgctt tctgccgacg actggcttga actggccggt tttgctttcg
    21781 ctcatcgtcc gctattaacg tcgttaggtt gcttaatgcg tctgttacaa accagcgaaa
    21841 tggcattacc ggcgctgcgt gggcgtttac agaaaaacgc cagcgacgcg cagttatgta
    21901 ccacacttaa actttcaggc cgtaagctgt tactggtccg tcagcgggaa gaggccgcgc
    21961 aggcgctata cgcacttgat gatgttcgca ctgagcgttt gcgcgatcgc ataacgcaat
    22021 ggcaattttt tcactgactc cttcaatttt ctcccatggt cattgtgagt cagcggcgta
    22081 aaattaatga catcagttaa ggagttcgcc atgaaacatg accattttgt tgttcaaagc
    22141 ccggataaac ctgcgcaaca gttgttactg cttttccacg gtgtcgggga taacccggtg
    22201 gcaatggggg aaataggtag ctggtttgcg ccgctatttc ctgatgcgct ggtggtgagt
    22261 gtcggaggcg cggagccgag cggtaatccg gcggggcgtc agtggttttc ggtgcagggt
    22321 attacggaag ataatcgcca ggcgcgtgtg gatgccatca tgccgacgtt tattgagacg
    22381 gtgcgctact ggcagaaaca gagcggagta ggggctaccg ccacggcgtt aatcggtttc
    22441 tctcaaggcg cgattatggc gctggagagc attaaagccg aaccgggcct tgcctcacgc
    22501 gtcattgcct ttaacggacg ctatgcgagc ctgccggaaa ctgcgtctac agccacgaca
    22561 attcacctga ttcatggcgg tgaagatccg gttatcgatc tggcgcatgc ggttgcggcg
    22621 caggaagcgt taatcagtgc cggtggtgac gtgacgctgg atatcgtgga ggatttaggc
    22681 catgcgatcg acaatcgcag tatgcaattt gctctggatc acttgcgtta taccattccg
    22741 aaacattatt tcgacgaagc gctaagcggc ggtaagcctg gcgatgatga tgtgattgaa
    22801 atgatgtaat aatagccggg taggtctgat aagactgtat caggcatcat ggccggatgc
    22861 ggcgttaacg ccttatccgg ccacttagct tacttcttcg gttgatcctt tttcggccag
    22921 tcgtcgtcat cgtcccactt atcgttgaaa tcacgatgcg ggggaagctc cggcttgttg
    22981 gcgagaaatt tcttatggtt gacgcgcttg agatctttaa tcacgttcag caggacgcct
    23041 accaaaaaga caatcaccag aatccaccaa tatttagcca gccagtccat gcttatttcc
    23101 tcttgcagaa ccactcatca ggcgacgagc tgttccatga tacgttgata catacgggca
    23161 agtagctgca ggtcggcagc gttcacacat tcattaattt tatgaatagt ggcattgacc
    23221 ggcccgagtt ccaccacctg cgcccccatg cgggcaataa agcgcccgtc ggacgttccg
    23281 cctgtggtta gtagctgcgg tttaatttca ttatagtgct caaccgcgtt aacgaccgca
    23341 tccaccagtt taccgcgcgc ggtcaaaaat ggctgcccgg aaagccacca atccaccgta
    23401 tagcgcagtt gatgtttttc aagcagggca agtacctgcg ctttgatcat ctcatcagtc
    23461 agttcggtgc tgaagcggaa gttaaactgc acaaacagtt cacccggaat aacgttgtta
    23521 ctgcccgttc ccgcctgaat attggcaatc tgcatactgg tcgccgggaa gaattcattg
    23581 ccctgatccc actcaatagc cactaattca ttaaggaaag gtgctgcgcg atgtaccgga
    23641 ttgtcagcca gatgtgggta ggcaacatgc ccctgcacgc catgaatggt gaggttgcag
    23701 gttaacgatc cgcgacgacc atttttcacc acatcaccaa ccacttcgat actcgacggt
    23761 tcgccaacca gacagtaatc gagacgctca ttacgcgcca ttaacgcttc gacgactttt
    23821 accgtaccgt tgtgggcact agcttcttca tcagaggtga tcagaaatgc cagtcgcccc
    23881 gtatggttgg gatgttgtgc gacaaaacgt tctgccgcta ccaccatcgc cgccagcgag
    23941 cctttcatat ctgccgcacc gcgcccgaat aacatgccgt cacgaatggt gggttcaaac
    24001 ggcggattga tccaacggtc ggcgtcgcca ggcggcacca cgtcggtatg cccggcaaag
    24061 gctaacgttt caccctgccc acgccatgcc caaaaattct gcgtatcggc aaagtccatg
    24121 cgttcaacgg taaaaccgat cgcctgcaaa cgttcaatca acaaagcctg gcatcctgcg
    24181 tcatcaggac tcagggaagg gcggcgaata agctgttgtg tcagctcaat aaccgggcac
    24241 gacatagact acacctcatg gaaaaattgc tgataactgg attcactgaa acccagcagc
    24301 ataggcttac caggcgcgca gagcaatgga cgtttgataa ttgcaggcat ttcagtcatt
    24361 aatgtcgccg cagaggccgc atcggtgatt ttattgcggg tggtttcgtc cagtttacgc
    24421 caggttgtac cacgggtgtt gagtaacgct tcccagccta attcgttgat aaaaccgttc
    24481 aataattcgc tgtccaatcc atcgacgcgg taatcatgaa agcgatagtc gatgttattg
    24541 gcttccagcc aacggcgagc ctttttaatg gtgtcacaat ttttgatgcc gtaaagtgta
    24601 accataatga atcctttttc gatttatgag aataaatact catttaaggg tgaaaacatt
    24661 ttgcctg
//
LOCUS       DAZGQL010000080        24475 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.080, whole genome shotgun sequence.
ACCESSION   DAZGQL010000080 DAZGQL010000000
VERSION     DAZGQL010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24475
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.080"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            172..942
                     /gene="yjjP"
                     /locus_tag="ACHKIR_003606"
     CDS             172..942
                     /gene="yjjP"
                     /locus_tag="ACHKIR_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HFO9991288.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTAAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            933..1406
                     /gene="yjjB"
                     /locus_tag="ACHKIR_003607"
     CDS             933..1406
                     /gene="yjjB"
                     /locus_tag="ACHKIR_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HFO9991289.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1514..2053
                     /gene="dnaT"
                     /locus_tag="ACHKIR_003608"
     CDS             1514..2053
                     /gene="dnaT"
                     /locus_tag="ACHKIR_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HFO9991290.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2056..2793
                     /gene="dnaC"
                     /locus_tag="ACHKIR_003609"
     CDS             2056..2793
                     /gene="dnaC"
                     /locus_tag="ACHKIR_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HFO9991291.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            2842..3336
                     /gene="yjjA"
                     /locus_tag="ACHKIR_003610"
     CDS             2842..3336
                     /gene="yjjA"
                     /locus_tag="ACHKIR_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein YjjA"
                     /protein_id="HFO9991292.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3590..5881
                     /gene="opgB"
                     /locus_tag="ACHKIR_003611"
     CDS             3590..5881
                     /gene="opgB"
                     /locus_tag="ACHKIR_003611"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HFO9991293.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWKGKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6019..7041)
                     /gene="lgoD"
                     /locus_tag="ACHKIR_003612"
     CDS             complement(6019..7041)
                     /gene="lgoD"
                     /locus_tag="ACHKIR_003612"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HFO9991294.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGALVAVADTSPARREHVATRLELPVLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            7180..8094
                     /gene="lgoR"
                     /locus_tag="ACHKIR_003613"
     CDS             7180..8094
                     /gene="lgoR"
                     /locus_tag="ACHKIR_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HFO9991295.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8309..9670
                     /gene="lgoT"
                     /locus_tag="ACHKIR_003614"
     CDS             8309..9670
                     /gene="lgoT"
                     /locus_tag="ACHKIR_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9991296.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLSFAEWRSLFRNRTMWGMMIGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IIAGMFCSAAFTLVVPQATTSMTAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(9719..11383)
                     /gene="tsr"
                     /locus_tag="ACHKIR_003615"
     CDS             complement(9719..11383)
                     /gene="tsr"
                     /locus_tag="ACHKIR_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HFO9991297.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAASEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVILAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
     gene            11565..12428
                     /locus_tag="ACHKIR_003616"
     CDS             11565..12428
                     /locus_tag="ACHKIR_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFO9991298.1"
                     /translation="MAYSIDIISCITERFMALTATEKRIAQFILDDVSAAVELPIAEL
                     ARLTQTSQASVTRFARALGCKDVRELKVKLAQSLAVGQRFILDVPDLDGVQGIYEAII
                     SVLETHRRSLDMNAISQAVSWLSQARQILALGTGGGSTICSQEIQYRLFRLGLPVVSQ
                     NDALMMRMMCSAVMPQDVVIVLSLGGYTPEIIESAAIARQYGARVIAITPAQTPLAEQ
                     VDLVLPLLVQESDYIFKPTPSRYAMLAMVDVLATELAMANKAQAKDRLRRIKLVLDSH
                     RGGANRQPLGD"
     gene            complement(12438..13907)
                     /locus_tag="ACHKIR_003617"
     CDS             complement(12438..13907)
                     /locus_tag="ACHKIR_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001084240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HFO9991299.1"
                     /translation="MNSHIFLVGFIIYAIAMIWLGWFVSRNQKSGEDFLLGGRSLPLF
                     LTLGSTVATMVGTGSSMGAVGFGYSNGWAGMLYGVGGAVGILLVAWLFAPVRKLRFMT
                     MSEELSYYTGGSHLIKNIVGLMIFIASIGWLGAHILGGSMYLAWATGIDLTVAKLIIA
                     LAFAIYVIIGGYSAVVWTDTIQALILFFGFILMAILAVVHVGGWSAIEQAMDPKAMSL
                     FAIDKMGVLPALSLALVIGVGVLATPSYRQRIYSGKDVPSVRRSFVITGVLYLFFSIL
                     PAIIGMAAFTMNPNLENSNYAFLFATSFLPAILGLVVLIAGLSATMSSASSDAIAAVA
                     IMMRDVYTMITGRMPPEDKAITYSRWMLTFVIGLALVFALTSNDIISYITKMISMLMS
                     GLFICSILGRFWLRFNWQGALAALVSGMVMSIVVLMNADWLAYWGNPCIPSVLGSLVA
                     GVLVTLVTPASQVSREEALAIITNERENQSVVITKPEEA"
     gene            complement(14010..14399)
                     /locus_tag="ACHKIR_003618"
     CDS             complement(14010..14399)
                     /locus_tag="ACHKIR_003618"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFO9991300.1"
                     /translation="MSIKRYGTEGGTGTGGQHLPFARAVEAGGWLYVSGQTPMKNGEV
                     VEGGIVEQSRLAIQNCVDIMTEAGYSLADVVHVKVILTDARYFQSFNKVFREFFGENP
                     PARICCVADLVVDCKVEVDVTCYNATR"
     gene            complement(14409..15851)
                     /locus_tag="ACHKIR_003619"
     CDS             complement(14409..15851)
                     /locus_tag="ACHKIR_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004100781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFO9991301.1"
                     /translation="MQVDWLIKNVTVIDGSGGPEFRGDVAITGDRIVDIAPALNVTAQ
                     QVIDGEGRVLAPGFIDVHTHDDINVIRIPEYLPKISQGITTVIVGNCGISAASAKMKG
                     EVPDPMNLLGEAEHFIYPTVESYAQAVEAARPSLNVGTLIGHTALRNNHMDDLFRPAT
                     VDEIAAMRADLRLALSQGALGLSSGLAYATAFQATTEEVMALAEELAGEKGVYTTHLR
                     SEFEPILDALDEAFRIGRHGKVPVVVSHHKCAGAKNWGRTKETLAFFDQMRQHQEIGC
                     DVYPYSASSSTLDLKQVTDEFDIVITWSQTHPQQAGKTLAQIAIDWQMSMLEAAKLLM
                     PAGAIYYNMDERDVRRVLSYPVSMIGSDGLPNDPMPHPRLWGAFPRVLGHYCRDEGLF
                     PLTTAIHKMTGLSASRFRLPQRGLVKVGYFADLVLFDPQTIRDVASFSDPKRPADGIE
                     AVMVNGVMSYGPDKHITGRAGRFLRRQTSH"
     gene            16052..17272
                     /locus_tag="ACHKIR_003620"
     CDS             16052..17272
                     /locus_tag="ACHKIR_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid deaminase"
                     /protein_id="HFO9991302.1"
                     /translation="MKYHSEPLVPHKSALMQMPANLLAEDVCLPAAIIKKQALENNIT
                     WMQRYADARGVSLAPHGKTTMTPWIFQAQQAAGAWAIGVGSAWQAGAAMASGIQRVLM
                     VNQLVGKANMQLIAQLQRHYPTVDFICCIDSIENARALSAFFASQQQTLNVMIELGVP
                     GGRCGCRSRDAALTLAKQAAQLPGLRLRGLELYEGVLHGDDPQPQVEALLRDAAQLAC
                     DMASLVDGEFILTGAGTVWYDVVCNIWLAAEKPDNCRIVIRPGCYITHDTGIYDAAQQ
                     QLLARDPVACDLAGDLTSALELVAMVQSVPEADRAVVNFGKRDCAFDAGLPQPVAHYR
                     NGKSLAFDPQAIRSTGIMDQHCMLQLSANSDVQVGDILVFGTSHPCLTFDKWKTLLLT
                     DDDYNVLAELDTLF"
     gene            17540..19690
                     /gene="btsT"
                     /locus_tag="ACHKIR_003621"
     CDS             17540..19690
                     /gene="btsT"
                     /locus_tag="ACHKIR_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HFO9991303.1"
                     /translation="MDTKKIFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            19740..19943
                     /gene="yjiX"
                     /locus_tag="ACHKIR_003622"
     CDS             19740..19943
                     /gene="yjiX"
                     /locus_tag="ACHKIR_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFO9991304.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            19954..20910
                     /gene="yjiA"
                     /locus_tag="ACHKIR_003623"
     CDS             19954..20910
                     /gene="yjiA"
                     /locus_tag="ACHKIR_003623"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HFO9991305.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEVEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPSRLLFQGV
                     QRLYSADWDRPWGDETPHSTMVFIGIQLPEDKIRAAFAGLRK"
     gene            complement(20944..21246)
                     /locus_tag="ACHKIR_003624"
     CDS             complement(20944..21246)
                     /locus_tag="ACHKIR_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HFO9991306.1"
                     /translation="MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDE
                     QWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKGYQTRLNAILREAMLRDQNKK"
     gene            complement(21261..21362)
                     /locus_tag="ACHKIR_003625"
                     /pseudo
     CDS             complement(21261..21362)
                     /locus_tag="ACHKIR_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463387.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
     gene            21559..22725
                     /locus_tag="ACHKIR_003626"
     CDS             21559..22725
                     /locus_tag="ACHKIR_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFO9991307.1"
                     /translation="MKNYKVITPLFPTYAQVKAMMKAVSGYSLKAVRNMITAIHEQTG
                     TPQKPVDWSEPDLWISERLTGEDADIARRIWDTDNHILNPRHSYGCYLFLNYPQFELM
                     ESTPDDTWQPTSRGQKFLQDDEKTLRSLDDQEGILQLLELLAGREMSRRADLLPEWQA
                     FLHQHSKFASASSVKSTLYSRLYNLIDRDMVDREGMSYRITDTGRAWLGKALPARKSD
                     PRKELLEAVKRYNEQQKELLREQISTMNPYKFEQLVGQLLEAMGYEQVEVTKASGDKG
                     VDVIGQVQVGITTITEVVQVKRMQNTITRPYIDQLRGALPYHKAIRGTLITTGKFAAK
                     CAEAALFPGAAPITLIDGDRLLELLIENNVGIRRSNAVELLDVDLQLFDELEMD"
     gene            22789..24408
                     /locus_tag="ACHKIR_003627"
     CDS             22789..24408
                     /locus_tag="ACHKIR_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003845607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFO9991308.1"
                     /translation="MAKAPTKKAKAKKGFEETLWDTANQLRGSVESSEYKHVVLSLVF
                     LKFISDKFEARRKKMIADGQADFLEMEVFYQQDNIFYLPEEARWSFIKQNAKQDDIAV
                     RIDTALSTIEKRNPTLKGALPDNYFSRQNLETKKLASLIDTIDNIETLAHETDVETLS
                     KEDLVGRVYEYFLGKFAATEGKGGGEFYTPKCVVTLLTEMLEPFQGKIYDPCCGSAGM
                     FVQSVKFVESHQGKSRDIALYGQELTATTYKLAKMNLAIRGLSANLGERPADTFFSDQ
                     HPDLKADYILANPPFNLKDWRNEAELTKDPRFAGYRMPPTGNANYGWILHMLSKLSAN
                     GTAGFVLANGSMSSNTSGEGEIRAQMIENDLIDCMIALPGQLFYTTQIPVCLWFMTKS
                     KAADPAKGYRDRQGETLFIDARNLGTMISRTTKELTAEDIATIADTYHAWRSTPEELA
                     ARIARGDSKLEKYEDQAGFCKVATLQDIKDNDYVLTPGRYVGAAEQEEDGVAFETKMR
                     ELSKTLFEQMKQAEELDRAIRQNLEVLGYGE"
BASE COUNT         5982 a         6470 c         6140 g         5883 t
ORIGIN      
        1 tttgtttgcg acgagagata attcgctttt taacaccgag cttctcccga taaccaggat
       61 taatcagcgc aattttgatc ttaacctgcc gcaaaacaac cgtgcttcat gatacaaatg
      121 gattctcatt atctgaacaa tacgcagcac gtctatgaca aagggcgagt tatgcaaact
      181 gagcaacagc gagccgtaac acggctttgt atccagtgtg gattatttct tttgcaacat
      241 ggtgcggaaa gcgcgttggt tgatgagctt tcctcacgac tgggtcgggc gttgggaatg
      301 gacagcgtcg aaagttctat ctcttcgaac gccatagtgc tgacaactat taaagatggg
      361 caatgcctga catcgacacg taaaaatcac gatcgcggca ttaatatgca tgtggtgact
      421 gaagtccagc acattgtgat tctggcggag caccatctgc tggattacaa aggcgtagag
      481 aaacgattta gccaaattca gccattacgt tacccaagat ggctggtagc cttaatggtt
      541 ggcctttctt gcgcctgttt ctgtaaactc aataacggtg gctgggatgg tgccgtcatc
      601 accttctttg ccagtacggc cgcgatgtat atccgccagc tgctggcaca acgtcatctt
      661 catccacaga tcaacttttg cctcaccgct ttcgccgcca ccaccatttc cggtttgctt
      721 ttgcaacttc ccactttcag caataccccc accatcgcga tggccgccag cgttctgctg
      781 ctggtgccgg gctttccgtt gattaatgcc gtcgccgata tgtttaaagg ccacatcaat
      841 accggactgg cacgctgggc gatcgccagt ctgctgacac tggctacctg cgtcggcgtg
      901 gtgatggcac tgacgatttg ggggctacgc ggatgggtgt gatcgaattt ctgttagcgt
      961 tggcgcagga tatgatcctc gccgccattc ctgcggtcgg ctttgcgatg gtgttcaacg
     1021 ttcccgtgcg ggcgttacgc tggtgtgcgt tgcttggctc gataggtcat ggttcccgaa
     1081 tgatcttaat gaccagcggg ctgaatattg agtggtcaac ttttatggct tctatgctgg
     1141 tcggcaccat tggtattcaa tggtcgcgct ggtatctggc gcatccgaaa gtgtttaccg
     1201 tggcggctgt tattcctatg ttccctggca tatcggctta taccgcaatg atttcggcgg
     1261 taaaaatcag ccagctaggt tacagcgaac cgttgatgat taccctgtta accaactttc
     1321 ttacagcttc atcgattgtt ggtgcgttat ccatcggtct ttccattcct ggattatggt
     1381 tgtaccgcaa gcgccctcgc gtataaaatt gcctcactta aagcacgcgg atgagagaca
     1441 gcctcctctc ctccgtgtgt tactataaaa gttatctccc ttctcgttca tcgttccata
     1501 tttgagaaac agtatgtctt ccagagtttt gaccccggac gtcgttggta ttgacgccct
     1561 ggtacacgat caccaaaccg ttctggcaaa agctgaaggc ggtgtggttg ccgtatttgc
     1621 taacaatgcc ccggcgtttt atgccgttac gcctgcacgc ctggctgaac tgctggcgct
     1681 ggaagaaaag ctggcgcgtc cgggaagcga tgtcgctctg gacgatcaac tctatcagga
     1741 accgcaagcc gctcccgttg ctgtacccat ggggaaattc gccatgtatc cggactggca
     1801 acccgatgcc gactttatcc gcctggcggc gctatggggc gtggcgctaa gagagccggt
     1861 gaccaccgaa gaactggcct cattcattgc ctactggcag gcggaaggta aagtctttca
     1921 ccatgtgcag tggcaacaaa aactggcgcg cagcctgcaa atcggtcgtg ccagcaacgg
     1981 cggactgccg aaacgagatg tgaatacggt cagcgaacct gacagccaaa ttccaccagg
     2041 attcagaggg taacgatgaa aaacgttggc gacctgatgc aacgcctgca aaaaatgatg
     2101 cctgcccata tcaaacctgc gttcaaaacg ggtgaagaac tactggcctg gcagaaagaa
     2161 caaggagcga tccgctccgc cgctctcgaa cgtgaaaatc gggcgatgaa aatgcagcgc
     2221 acctttaacc gctccggtat tcgtccactg catcagaact gctcctttga gaactatcgc
     2281 gttgagtgtg aagggcagat gaatgcgtta agcaaagcgc gccagtatgt cgaagagttt
     2341 gacggcaaca tcgccagctt catcttttct ggcaagcccg gaaccggcaa aaaccatctg
     2401 gcggcggcaa tctgcaacga gctgctgcta cgcggtaaat ccgtattgat catcaccgtg
     2461 gccgatatta tgtcggcgat gaaagatacc ttcaggaata gcggtaccag cgaagaacaa
     2521 ctgcttaacg atctgagcaa cgttgatctg ctggtgatcg atgagatcgg cgtgcagacc
     2581 gaatcgaaat acgaaaaagt gatcatcaac cagatcgtcg atcgccgctc ttcttccaaa
     2641 cgcccaaccg ggatgctgac caacagtaat atggaagaga tgaccaagtt gctgggcgaa
     2701 cgcgtgatgg accgtatgcg cctgggtaac agtttgtggg tgatcttcaa ctgggatagc
     2761 taccgtagcc gggtaacggg taaagagtat taatccccgt ttctggcggt atactgcgac
     2821 acaattccac cttgagtaat gatgatgaaa actgttaaac atcttctgtg ctgtgccatt
     2881 gccgccagcg cattaatttc caccggggtg catgctgcgt cctggaaaga tgcgctctcc
     2941 agcgccgcca gcgaacttgg caaccaaaac agcacgacac aggaaggcgg ttggtcgctc
     3001 gcgtcattaa ctaacttgct tagcagcgga aaccaggcct taagcgcaga taacatgaac
     3061 aatgccgcag gcattctgca atactgcgcg aagcaaaagc tggcttcggt aaccgacgcc
     3121 gaaaacatca agaatcaggt actggaaaaa ctgggcctga acagtgaaga gcaaaaagaa
     3181 gacaccaact atctggatgg cattcagggt ttgctgaaaa ccaaagatgg tcagcaactc
     3241 aatctggata atattggaac gactccgctg gcagaaaagg tgaaaaccaa agcctgcgat
     3301 ctggtgttaa agcaggggct gaacttcatt tcctgaaccg cgaaacgccg cgttttagct
     3361 gttacccggc tgacgcggct ctctcctgcc cttttccctg ctcctggacg gttttccccc
     3421 tgttacacgg gaagcgaccg cgatcaaaaa agtctgttta taaactaatt cctaacaaca
     3481 tcgcattttt ttgacactag aattgcagca atatggcgac gccattacat tgtgcttctc
     3541 aaaaagctta aacgttggct aaacggcccg gtttagtgag gatcgcccgt tgtcagaact
     3601 actctctttc gccctttttc tcgcctctgt gctgatttac gcatggaaag cgggacgtaa
     3661 cacctggtgg tttgcagcca cattaacggt actggggcta tttgtcgttt taaatatcac
     3721 cctgtttgcc agcgactatt ttactggcga tggtattaac gacgcggttc tctatacctt
     3781 aaccaacagc ctgaccggtg ctggtgtcag taaatacatc ctgcctggta tcggcattgt
     3841 gctggggctg acagcggtgt tcggtgcgct gggctggatc ctgcgccgtc gtcgtcatca
     3901 tccgcaccat tttggttaca gcctgctggc actcttactg gcgttgggtt cagtggatgc
     3961 cagcccggca tttcgtcaga taacggaact ggtgaaatcc cagtcacgcg acggcgaccc
     4021 ggactttgcg gcttattata aagagccgtc gaaaactatc cctgacccga aactcaacct
     4081 ggtttatatc tacggcgaaa gtctcgagcg gacctatttt gataacgagg ctttcccgga
     4141 tctcacgcct gaactgggcg cgttgaaaaa tgaaggcctg gatttcagcc acacgcagca
     4201 gctgccagga acggattaca cgattgcggg catggtggct tctcagtgcg gaataccgct
     4261 gtttgccccc tttgaaggca acgcctccgc ttctgtttcc agcttcttcc cgcagaacat
     4321 ctgtctgggc gatatcctga aaaactcggg ttatcagaac tatttcgtgc agggcgcgaa
     4381 tctgcgtttt gccggtaaag atgtgttcct gaagtcgcat ggcttcgacc acttatatgg
     4441 ctcagaagag ctgaaaagcg tggtggccga cccgcactat cgcaacgact ggggattcta
     4501 cgacgatacc gttctcgacg aagcgtggaa aaagtttgaa gagctttccc gctcaggtca
     4561 gcgattctca ctgtttaccc tgacagtcga tacccatcac ccggatggtt ttatctcgcg
     4621 tacctgtaac cgcaaaaaat atgattttga cggtaagccg aatcagtcat tcagcgcggt
     4681 aagttgcagc caggagaata tcgcgacgtt tatcaacaaa atcaaagcgt caccgtggtt
     4741 taaagatacc gtcatcgtcg tctcttctga ccatttagcg atgaacaaca cggcgtggaa
     4801 ataccttaat aaacaggatc gcaataacct gttttttgtc atccgtggcg acaagccgca
     4861 gcaagagacg ctggcggtga agcgtaacac gatggataac ggcgcgacgg tgctggacat
     4921 tctcggtggc gataactatc tcggacttgg tcgtagcagt ttatccgggc agtcaatgtc
     4981 ggaaatcttc ctcaatatca aagagaaaac attggcgtgg aagccggata tcatccgcct
     5041 gtggaaattc cctaaagaga tgaaagagtt caccatcgac cagcagaaaa acatgattgc
     5101 cttctcgggt agccatttcc gtttgccgct actgttgcgg gtttcagaca aacgcgtgga
     5161 accgctgccg gaaagtgaat actcagcacc gctgcgtttc cagctggccg atttcgctcc
     5221 acgcgacaat ttcgtctggg ttgaccgttg ctacaagatg gcacaacttt gggctccgga
     5281 actggcactc tccaccgact ggtgtgtctc gcaagggcag cttggcggtc agcaaattgt
     5341 ccaacatgtt gacaaaacaa catggaaggg caaaacggca tttaaagata cggtcatcga
     5401 catggcgcgt tacaaaggca atgtcgatac gctgaagatt gttgataacg atattcgcta
     5461 caaagccgac agtttcatct ttaacgtcgc cggtgcgcca gaagaggtga aacagtttag
     5521 cgggatttcc cgtccggagt cgtggggccg ctggtccaac gctcagctgg gcgacgaagt
     5581 aaaaatcgag tacaagcatc cgctgccgaa gaaatttgac ctggtgatta ccgccaaagc
     5641 atacggcaat aacgccagcc gtcctattcc ggtacgcgta ggcaatgaag aacaaactct
     5701 tgtgctgggc aatgaagtga ccaccaccac gctgcatttc gataacccaa ccgatgccga
     5761 cacgctggta attgtgccgc cggaacctgt ctcaaccaac gaagggaata tcctcggaca
     5821 ctcgccgcgt aagctcggga tcggcatggt ggaaattaaa gtggtagaac gtgaagggta
     5881 attcctgact gatgtgtagg tcgcataagg tgcgacctac acgccttctc cggcttacaa
     5941 cacactatta agaaacaaaa aaaacagcgc ccaacacttc cccggacgct gcatggcaac
     6001 tttaataatc tcgccttatc agaaagtaat tacaccctta atcaactcac gattgttaat
     6061 cacatcacgc tcgtaggttt ctgccagcgt ggcgaacggg tagcgatggg ttaacatcat
     6121 gtcggcagtg attttccctt ccgccatcag tcgaccgact ttagcgaaat cttccggcgt
     6181 ggcgttgcgg ctacccatca tcgtcgtttc ttttttatgg aattctggat cggagaactg
     6241 caactcacct ttaaacagac cgacaaatac caccgtgccg ccgtgacgaa tcagattcac
     6301 ggtgttattc atcgcatgtt gattacctgt cgcgtcgatc actttctgcg ccagcgaacc
     6361 accaaactgc gcccgcagct gcgcgtcaaa atcttcggct gatgggtcca gcacaggcaa
     6421 ctccagacgc gttgccacat gttcacggcg cgccggactg gtatccgcca ccgctaccag
     6481 tgcgccatcg gctttagcga ttgccgccgc gcccagaccg attggccccg ccccgaccac
     6541 cagcacctgc tcgccgggag caatggctgc gcgacgcacc gcatgagcgc taatagcgaa
     6601 aggttcaatc aatgctgccg cctgcgggtc aataccgtct gcgggcaaaa tgttcgccac
     6661 cggcacgctc aaatactcac taaaaccgcc atcctgatgc acgccaatga ctgaaatttt
     6721 ttcacagcaa ttggtacgcc cgcttttaca cgccgggcat tgctgacagg caacataagg
     6781 gatcacagca acttgctgac catttttaag atcagcaata tttttaccca gcccaacaat
     6841 ctccccacat atttcatggc ctaaaacgcg tggataacta aaaaatggtt gatttccacc
     6901 ccaggcatga atatcggtac cgcaaatccc gacagactta atttttatta atgcttcatt
     6961 gtcacccgga ataggtatct cgcgttgttt ccagactaat tctttcggct gctggcaaat
     7021 taaaacattc atcgtagaca tgattcggct ctccattttt tgttgcctta gatatcaatg
     7081 aaaatgaaaa atggcagcgg aacggaaaat ctgttttgtg aaaacacacg cataaaaatg
     7141 ttttaaatcg ggttttaatg gactcacaag gaggaaaata tgagtcgttc acaaaattta
     7201 cgccacaatg tgattaacca ggtcattgat gatatggcgc ggggtcatat cccttcccca
     7261 cttccatcgc aaagtgcgct ggcggaaatg tacaacatca gccgcactac agtgcgtcat
     7321 attctcagcc acttacgcga atgcggcgtc ttaacgcagg ttggaaacga ctatgtgatt
     7381 gcgcgtaaac ctgaccatga tgacggtttc gcctgtacta ccgcttcaat gagtgaacaa
     7441 aacaaagtgt ttgagcaggc ttttttcacg atgataaacc agcgccagtt acgcccaggg
     7501 gaaacttttt ctgaactgca actggctcgg gccgcgggtg tcagcccggt tgtagtaaga
     7561 gaatatcttt taaaattcgg ccgttataat cttattcaaa gcgaaaagcg cggccagtgg
     7621 agcatgaagc aattcgatca atcctacgct gagcagctgt ttgagctacg cgaaatgctc
     7681 gaaacacatt cacttcagca ctttctcaac ctgcctgatc atgatccccg ctggctgcag
     7741 gcgaaaacca tgctcgaacg tcatcggtta ctgcgtgaca acatcggcaa tagctttcgc
     7801 atgttctcgc aacttgacag agattttcac tccttactgc tttcagctgc cgacaatatc
     7861 ttttttgatc aatcgcttga gatcatctca gtcatctttc attttcacta tcaatgggac
     7921 gaaagcgatc ttaaacagcg caatatcatt gccgtcgatg agcatatgac cattctcagt
     7981 gctctgattt gccgcagtga ccttgatgcc acccttgcgc tccgtaacca tttgaattca
     8041 gctaaacaat caatgattcg atccattaat gagaacacgc gttatgctca ttaagtacgg
     8101 attaaaaaca gcaaactatt agcgggtgca cggttttatc aaaagataaa taccgggaaa
     8161 ttcccgctta cctatgctca caatcagtcg actaaaaatg tttttgtgag cggtagtaaa
     8221 gtcctaaaac tttaacctgc cgttaatgtc aaccttgcgt aatgcacaat tacgtaaaca
     8281 attaaaatta taaatacagg agtccggcgt ggaaaaagaa aatatcaccc tcgatccgcg
     8341 ttcttcattt actccatctt cgtcggcaga tattcccgtg ccaccagatg gattagttca
     8401 acgcagtacc cgaattaaac gcattcaaac caccgccatg ttgttattat tttttgcggc
     8461 ggtaatcaat tatctcgacc gcagttcgct gtcggttgca aatttaacga ttcgtgaaga
     8521 attgggatta agtgctaccg aaatcggcgc tttgctctcc gtgttttcac tcgcttacgg
     8581 gattgcgcaa cttccttgcg gcccactatt ggatcgtaaa ggcccacgcc tgatgctggg
     8641 actggggatg ttcttctggt cactgttcca ggccatgtct ggcatggtgc acagctttac
     8701 gcagttcgtg ttggtgcgta tcggtatggg gattggtgaa gcgccgatga acccatgcgg
     8761 tgtaaaagtc attaacgact ggttcaacat caaagagcgc ggacgcccga tgggcttctt
     8821 caacgcagct tctactattg gcgttgccgt aagcccaccg attctggcgg cgatgatgct
     8881 ggtgatgggc tggcgtggga tgtttattac cattggtgtg ctggggattt ttctcgccat
     8941 tggctggtat atgctctatc gcaaccgcga gcacgtagaa ctgactgccg ttgaacaagc
     9001 ttacctcaat gctggtagcg tcaatgcccg ccgagatccg ctcagttttg ccgaatggcg
     9061 cagcctgttc cgtaaccgca caatgtgggg aatgatgatc ggattcagtg gcatcaacta
     9121 cactgcgtgg ctgtatctgg cctggcttcc tggttacctg caaacagcct ataacctgga
     9181 tttaaaaagc acagggttga tggcggctat ccctttcctg tttggggctg ccgggatgct
     9241 ggtcaacggt tacgttaccg actggctggt caaaggggga atggctccga ttaaaagccg
     9301 taagatctgc attattgccg ggatgttctg ttctgccgcc tttacgctgg tcgtaccgca
     9361 agcgacaaca tccatgacag cggttctgct gattggcatg gcactgtttt gtattcactt
     9421 tgccggaaca tcctgctggg gcttgatcca cgtcgcagtt gcttctcgca tgacggcgtc
     9481 ggtgggcagt atccagaact ttgccagctt catctgcgcc tctttcgcgc cgatcattac
     9541 tggttttatt gttgatacca cccactcatt ccgtctggca ctaatcatct gcggttgcgt
     9601 caccgcagcg ggggcactgg cgtacatctt cctggttcgt cagccgatca acgacccgcg
     9661 taaagattaa actccctatg cccgataaca ttttgcttat cgggcatttt tatggcgatt
     9721 aaaacgtttc ccaattctcg ccgctatctg ccacggccat tttacgcggc gtagctggcg
     9781 tcacggtttt tactacagcc gatgtttcac gctgctgttg ctgctgttgc tgaatccgga
     9841 acactgccac tgcttcggtc aggcgactgg cctgctcttc cagcgcggcg gcggcagcgg
     9901 cagattcttc caccagcgcg gcgttttgtt gggttacccg gtccatctca gcaaccgcta
     9961 agccaacctg atcgatacca cggctctgct catcagaagc agaagcaatt tcgcccataa
    10021 tgtccgtcac gcgggtcacg gcactgacaa tctccgccat tgtttccccg gcgctttcga
    10081 ccagcgtaga gccaacatcc actttcccca ccgagtcttc aatcaggctt ttaatttcac
    10141 gagcagcctg agcgctacgc tgggccagat tacgcacttc tcccgcaacc accgcaaaac
    10201 cgcgcccttg ctcacccgcg cgcgctgctt caaccgccgc gttcaaagcc agaatattgg
    10261 tctggaaggc aataccgtca attacgctga taatatcggc gattttctgc gaactggtgg
    10321 agatatcgcg catagtctgc accacgttat ccaccacttt gccgccgcgt tgcgccgttt
    10381 cggaagcact taacgccagg tggctggcct gacgcgcatt ttcggcgttc tgcttcaccg
    10441 ttgcggtcag ttgctccatg ctggctgccg tctcttccag cgaagcggcc tgttgctcgg
    10501 tgcgcgaaga gagatcgtta ttgccggtgg cgatttcgct ggcaccgcta tagatggcat
    10561 tggccccgtt gcgcacatca ccgacggtac gcatcagctc tccctgcata tggcgcaaac
    10621 tctctgccag ttgccccatc tcattagagc catccacctc aatcggtttc accagatcgc
    10681 cgcctgcaat atgacgaatg ctgtcaatca ggcgattcat tggcgcgacc agcgaggctt
    10741 taataccgaa ccagacggcg aggatgaccg ccagtacgac gatcatcacg cctaccagaa
    10801 tccacatcgc ctggctgtag gaggcattgt tatcgctgac ggcgatatca tagagccgat
    10861 cgttttgctc catgtaagcc acatactgct tctcgaaacc gtcctgatat ccctgggtcg
    10921 gttgatcaaa gaactcgttg attttgcctg cacctaacag ttggatcagc tccgccagcg
    10981 cattgtgata aatatcgtaa ttacgtttga tctctgacgc tgcggcggtg ctctgacgcg
    11041 ggtcacgcgg caacgcttcg taatccgccc agtttttttc cgcctgtttc agcgaaatac
    11101 tggcactctg catcagctca gcaacggttg aaccgctacc aatattattc tgatccatca
    11161 tgtagcggat acccgcgcgg ttgagggtgt tacgcgtctg caacaacgca acccagctgc
    11221 cattcagcgt ggattgctgc tggcgaatgg tttgtaaaac agtgaaattt tctttgtcat
    11281 tctttaaggc attaaagaac agaccgcctg atgtcagttg taaaaggcca aaaacggcca
    11341 aaaccagcag taagctggtc acgatcttga tacgttttaa catggtttct ctttccacta
    11401 gacagataca ctggttgtcg gcctggtggt ggaaaacttt atgctgtgga aaagcgtcgg
    11461 cactgctttt gttgcagcaa acttgcgttt tttgtgaggg tcacactcta tagcggcagt
    11521 ttatggtaca aagtatcaat cttcttcgaa ccagtgatac tcctatggct tatagcatcg
    11581 atatcatctc ctgcattacc gaacggttta tggcgttaac agccactgaa aagcgtatcg
    11641 cacaatttat tcttgatgac gtctctgccg cagtagagtt gccaattgct gagctggcac
    11701 ggttaacgca aaccagtcag gcgtcggtga cgcgttttgc ccgtgcactg gggtgcaaag
    11761 atgtccgcga gttgaaagtc aaactggcgc agtcactggc tgtcgggcag cggtttattc
    11821 tcgatgtacc ggatcttgac ggtgttcagg ggatctacga agcgattatt agcgtgctgg
    11881 agacgcatcg tcgttcgctg gatatgaatg ccatcagcca ggctgtaagc tggttaagtc
    11941 aggcgcggca aattcttgcg ttaggaactg gcggcggttc cactatctgt tcacaggaga
    12001 ttcagtaccg tttattccgt ctggggctac cggtggtcag tcagaatgat gccctgatga
    12061 tgagaatgat gtgctcggca gtcatgccgc aggatgtggt gattgtgctt tcgctgggcg
    12121 gttatacacc ggaaatcatc gaaagtgcgg caatcgcccg tcagtatggc gcgcgtgtta
    12181 ttgccattac gccagcacaa acaccgctgg cggaacaggt ggatctggta ttgccgttac
    12241 tggttcagga aagtgattac atctttaagc ccaccccttc ccgctacgcg atgttagcca
    12301 tggtcgatgt actggcaaca gaactggcaa tggcaaataa agcgcaggcg aaagatcgcc
    12361 tgcggcggat aaaactcgtg ttagacagcc accgtggggg cgctaaccgc cagccacttg
    12421 gcgattagca ttgtcggtta tgcttcttca ggtttggtga tcacgacact ctggttttca
    12481 cgctcattgg tgataatagc caaagcttct tcccggctga cctgacttgc tggcgttacc
    12541 aacgtcacca gtacgccagc gaccaggctg cccagcactg acggaataca cgggttaccc
    12601 cagtaagcca gccagtcggc attcatcagc accacaattg acatcaccat accgctcact
    12661 aacgccgcca gcgcgccctg ccagttaaag cgtagccaga agcgaccgag aatcgagcag
    12721 ataaacagcc cggacatcag catcgaaatc attttggtaa tgtagctgat gatgtcatta
    12781 gaggtcagag caaagaccag cgccaggcca ataacaaagg tcaacatcca gcgtgagtaa
    12841 gtaatggcct tgtcctccgg cggcattctg ccggtaatca tggtgtagac gtcgcgcatc
    12901 ataatcgcca ccgccgcgat ggcatcggag ctggcagaag acatggtggc tgaaagcccg
    12961 gcaatcagca ccaccagccc aagaatggca ggtaagaaac tggtcgcgaa caagaaggcg
    13021 tagttgctgt tttccagatt tgggttcatg gtgaacgccg ccatgccgat aatggcaggc
    13081 aaaatcgaga agaacagata taacacccca gtaataacaa acgaacggcg tacagaggga
    13141 acatctttac ccgagtaaat acgctggcga taagacggtg ttgccagcac acctacgcca
    13201 ataaccagtg ccagcgaaag cgcaggcagc acgcccattt tatcaatcgc aaacaggctc
    13261 atcgcttttg ggtccatcgc ctgctcaatc gcactccagc cgccaacgtg aacaacggca
    13321 agaatagcca ttaatataaa gccaaagaac agaattaggg cctgaatagt atctgtccac
    13381 actaccgccg aataaccgcc gataataaca taaatcgcaa aagctaaggc gataattaat
    13441 tttgccacag taagatcaat acccgtcgcc caggcaagat acatacttcc acctaatata
    13501 tgcgccccca gccagccaat agaggcaata aatatcatca ggccgacaat atttttaatt
    13561 aaatgactac cgccagtata ataagagagt tcttcactca tggtcataaa acgtaattta
    13621 cgtaccggtg caaacagcca cgccaccagc aatataccta cggctccacc gacgccataa
    13681 agcattccgg cccagccatt gctgtagcca aagcccactg cccccatact ggaacccgtg
    13741 ccaaccatcg tggcaacggt tgaccccagc gtcagaaaca gcggcagcga acgcccgccc
    13801 agcagaaaat cttctccgct tttctgatta cgagaaacaa accaaccaag ccaaatcatg
    13861 gcgatggcat aaataataaa gccgactaaa aagatatgac tgttcataat tcacatcctg
    13921 ccagtagaga gaaattaatg taagagttcc cggagtgctg aatgtattca gcactcaatt
    13981 gcggtctgtt tttttaattc ttaaatttat tagcgggtcg cgttataaca ggtcacatcg
    14041 acttcgactt tacaatcgac aaccagatca gccacacaac aaatacgtgc aggcggattc
    14101 tcgccaaaaa attctctgaa tactttatta aatgactgga aatagcgggc gtcggtcagg
    14161 atcactttca cgtgaaccac atccgccagg ctataaccgg cttcagtcat gatgtcgaca
    14221 cagttttgga tcgccagtcg tgactgttca acgattccgc cttctaccac ttcaccgttt
    14281 ttcatgggtg tttgcccgga gacgtacagc cagccgcccg cctcaaccgc cctggcaaaa
    14341 ggcaaatgct gcccgccagt gcctgttcca ccttccgtac cgtaacgttt aatgctcata
    14401 ttcactcctt aatgtgaggt ctgccggcgc aggaagcgtc ccgcacggcc tgtaatatgt
    14461 ttatcgggac catagctcat aacgccgttc accatcacag cctcaatgcc atctgccggg
    14521 cgtttgggat cagaaaagct ggcaacatca cgaatggttt gtggatcgaa caacaccagg
    14581 tccgcaaaat aaccgacttt caccagtcca cgctggggca ggcgaaaacg gctggcagaa
    14641 agcccggtca ttttgtggat agccgtggtc agtgggaata agccttcatc acggcaatag
    14701 tggcccagca cgcgagggaa ggcgccccat aaacgtggat gcggcatggg atcattgggc
    14761 aggccgtcag agccaatcat actgaccgga taactcaaca ctcggcggac gtcgcgctcg
    14821 tccatgttgt aatagatagc cccggcaggc attagcagct tcgcggcctc cagcatactc
    14881 atctgccagt cgatggcgat ttgcgccagt gttttcccgg cctgctgcgg atgtgtttgt
    14941 gaccaggtga tcacaatatc gaattcgtcg gtgacttgtt tgagatccag cgttgaagag
    15001 ctggctgagt aagggtagac atcgcagcca atttcctggt gttgacgcat ttggtcaaaa
    15061 aaggccaacg tttcttttgt ccgcccccag tttttcgccc cggcgcattt atggtgggaa
    15121 accacgacag gcactttgcc atgacgccca atacgaaacg cctcatccag ggcatcgaga
    15181 atcggttcaa attcagaacg cagatgggtg gtataaacgc ccttctcacc ggctaattct
    15241 tccgccagcg ccatgacttc ttcggtagtc gcctgaaatg ccgttgcata ggccagcccg
    15301 gaacttaacc ccagcgcccc ctgactcaac gccagacgta agtcggcacg catagcggca
    15361 atttcatcca ccgtcgccgg gcgaaacagg tcgtccatat ggttattacg cagcgccgta
    15421 tgaccaatca gcgtgccaac gttcagtgac ggcctggctg cttccactgc ctgggcatag
    15481 ctttcaacgg taggataaat aaagtgctct gcttccccca acagattcat cgggtcagga
    15541 acttcacctt tcattttcgc cgacgccgca ctaatcccgc agttaccgac aatcaccgta
    15601 gtgatcccct ggctgatctt tggcagatat tccgggatgc gaataacgtt gatatcgtca
    15661 tgggtatgaa cgtcgataaa cccgggtgcc agcacccgcc cttccccgtc aatgacctgc
    15721 tgcgccgtaa cgttaagcgc aggggcaata tcgacaatcc gatcacctgt tattgccacg
    15781 tcaccgcgaa attcagggcc gccgctaccg tcgataacgg tcacattttt gattaaccag
    15841 tcaacctgca tgttcgtcac tccatttctg caatggtgac aagttaacca tttacaagga
    15901 cgaaaaacag tggaaaacta cgcaaaaccc agatgatttt atgatacttt tattcataaa
    15961 taataagata aataaactaa atatttatat ttcatttagt tacaaatgat actcacatcc
    16021 gatacggtga aaaacttaaa aggtaaatgt tatgaaatac cactcagaac ctctggttcc
    16081 ccataaatct gccctgatgc aaatgcccgc aaatctcctt gccgaagatg tctgtttacc
    16141 tgccgcgatc attaaaaagc aggccctgga gaacaacatt acgtggatgc aacgctacgc
    16201 tgacgcgcgc ggcgtttcac tggcaccgca cggtaaaacc accatgacgc cgtggatttt
    16261 tcaggcgcag caggcggctg gtgcctgggc aataggtgtc ggtagcgcat ggcaggctgg
    16321 tgcggcaatg gcgagcggta ttcagcgagt gctgatggtt aaccagctgg tcggcaaggc
    16381 gaatatgcag ttgattgccc agttgcaacg acattacccg acggtcgatt ttatctgctg
    16441 tatcgacagc attgagaacg cccgcgcttt gtctgccttt tttgccagtc agcaacagac
    16501 actgaacgtg atgattgagt tgggtgtccc cggcgggcgc tgtggttgcc gctcaaggga
    16561 tgccgcactg acactggcta aacaggcagc ccaattaccg gggttacgcc tgcgggggct
    16621 tgagttatat gaaggcgttt tacacggtga cgatccacaa ccgcaggtag aagctctgct
    16681 gcgggatgcc gcacaactgg cctgtgatat ggcaagtctg gttgatggcg agtttattct
    16741 caccggagca ggcacggtct ggtatgacgt ggtgtgcaat atctggctgg cggcagaaaa
    16801 gcctgataac tgccgcattg ttattcgccc tggctgctac atcactcacg acaccgggat
    16861 ctacgacgcg gcacaacaac agttgcttgc ccgtgatcct gtggcctgcg atctggcagg
    16921 cgatctcaca tctgcgctgg aactggtcgc catggtgcaa tcggtgccgg aagctgatcg
    16981 tgcggtggta aattttggta agcgtgattg cgccttcgac gccggactgc cgcaaccagt
    17041 cgcccactac cgcaacggca aatcactggc atttgatcca caggcgattc gcagtacagg
    17101 tattatggat cagcactgca tgttgcaact gtcagctaat agcgacgtgc aagtggggga
    17161 tattctggtg tttggcacat cgcatccgtg cctgaccttc gacaaatgga aaacgttgtt
    17221 attgactgat gacgactaca acgtactggc agaattagac actctcttct aagaacgccc
    17281 cttcggttaa ttatcgaagg ggcattttta ttgtgatctc aatcacatat ccacccgacg
    17341 aataattctt gcccaaccgc tcaccgccat tcctgaccgc ttacatccct aaaataacca
    17401 ctcagttatt taccttactt tacgcgcgcg taactctggc aacatcactg caggatagcg
    17461 gtcaatttac ctcctcaaac gcaacgcaaa cctagaacgg cttcggccaa ctattaatca
    17521 atacatgcca ggttttacta tggatactaa aaagatattc aagcacatac cctgggtgat
    17581 tctcggaatc atcggtgcat tctgcctcgc ggtagttgca ttacgtcggg gggagcacgt
    17641 cagtgccctg tggatcgtgg tcgcctctgt gtcggtgtat ctggtggctt atcgctacta
    17701 cagtctgtac atcgcccaga aggtgatgaa actcgacccc acgcgcgcga cgcctgcggt
    17761 tattaacaac gacggtctga actatgttcc gaccaaccgt tacgtgttgt ttggtcacca
    17821 cttcgccgct atcgccggtg ctggtccgct ggttggtccg gttctcgccg cgcagatggg
    17881 ctacctgcct ggcacgctgt ggctgctggc gggggtagtg ctggccggtg cggtacagga
    17941 ctttatggtg ctgtttatct cctctcgccg taacggcgca tctctcggtg agatgatcaa
    18001 agaagagatg ggaccagtac cggggactat cgcgctgttt ggctgtttct taatcatgat
    18061 catcatcctt gccgtcctgg cgctgattgt ggttaaagcc ctggccgaaa gtccgtgggg
    18121 tgtcttcacc gtttgctcaa ccgtaccaat tgcgctgttt atgggtatct acatgcgctt
    18181 tatccgtccg gggcgtgtgg gtgaagtctc tgtcattggt atcgtgctgc tggttgcctc
    18241 tatctacttc ggtggcgtga ttgctcacga tccgtactgg ggtccggcac tgacctttaa
    18301 agacaccacc attaccttcg cgctgattgg ctatgcgttt gtttccgcgc tgctgccggt
    18361 gtggctgatc ctcgcaccac gcgactatct ggcaaccttc ctgaaaatcg gcgttatcgt
    18421 cggcctggcg ctgggtatcg tggtgctgaa cccggaactg aaaatgcctg cgatgaccca
    18481 gtacattgac ggtactggcc cgctgtggaa aggcgctctg ttcccgttcc tgttcatcac
    18541 catcgcctgt ggtgcggtat ctggcttcca cgcgctgatc tcttccggta cgacgccgaa
    18601 actgctggct aacgaaaccg acgcgcgttt cattggctac ggcgcaatgc tgatggagtc
    18661 cttcgtggcg attatggcgc tggttgctgc gtccatcatc gaaccgggtc tttacttcgc
    18721 gatgaacacc ccgcctgctg gccttggcat caccatgcct aacctgcatg aaatgggtgg
    18781 cgagaacgcg ccgatcatca tggcgcagtt gaaagacgtt accgcacacg cggcggcgac
    18841 cgtcagctcc tggggcttcg tgatttcgcc agagcagatc ctgcaaaccg cgaaagacat
    18901 tggtgaacct tctgtcctga accgtgcagg tggtgcgccg acgctggcgg taggtatcgc
    18961 gcacgtgttc cacaaagtgc tgccgatggc tgacatgggc ttctggtatc acttcggtat
    19021 tctgttcgaa gccctgttca tcctgaccgc gctggatgcg ggtacccgtt ctggccgctt
    19081 tatgctgcaa gacctgctgg gtaacttcat cccgttcctg aaaaaaaccg actctctggt
    19141 tgctggtatc atcggtactg cgggctgtgt gggtctgtgg ggctacctgc tgtatcaggg
    19201 cgtggtcgat ccgctgggcg gcgttaagag cctgtggccg ctgttcggta tttccaacca
    19261 gatgctggca gccgtagcac tggtactggg caccgttgtg ctgattaaga tgaagcgcac
    19321 ccaatacatc tgggtaactg ttgttccggc tgtatggctg cttatctgca ccacctgggc
    19381 gctgggcctg aaactgttca gcaccaaccc gcagatggaa ggcttcttct acatggcaag
    19441 ccagtacaaa gagaagattg ctaacggtac tgacctgacg gcacagcaga ttgccaacat
    19501 gaaccacatc gttgtgaaca actacactaa cgcgggtctg agtattctgt tcctgattgt
    19561 ggtgtacagc atcatcttct acggtttcaa aacctggctt gcggtgcgta acagcgacaa
    19621 acgtactgac aaagaaactc cgtacgttcc aatcccggaa ggcggcgtga agatctcttc
    19681 gcaccactaa ccgtgtttag ccccgcttcg gcggggcttt gttcttatca gagtgaacta
    19741 tgtttggtaa cttaggacag gcaaaaaaat atctcggcca ggcggcgaag atgttgattg
    19801 gcattccaga ctacgacaac tacgtcgagc atatgaagac caaccatccc gacaagccgt
    19861 acatgagcta tgaagaattc ttccgcgaac gccagaacgc gcgctacggc ggcgatggta
    19921 aaggcggtat gcgctgttgt taatggagag agcatgaacc cgattgcagt taccctactc
    19981 accggttttt taggcgcggg caaaaccacc ctgctgcgcc atattcttaa tgaacagcac
    20041 ggctacaaaa ttgccgtgat tgaaaacgaa ttcggcgaag tctccgttga tgatcaactg
    20101 attggcgacc gcgccacgca gatcaaaacg ctgaccaacg gctgcatctg ctgttcgcgc
    20161 tccaacgagc tggaggacgc gctactcgac ctgctggaca atctcgacaa gggcaatatt
    20221 cagttcgacc gtctggtcat tgaatgcacc ggcatggccg atcccggccc gattattcag
    20281 acctttttct cccatgagat tttatgccag cgttacctgc tggacggcgt gattgcgctg
    20341 gtggatgcgg tacatgccga tgagcagatg aaccagttca ccatcgccca gtcccaggtg
    20401 ggctacgctg accgtattct gctgactaaa accgacgtcg caggcgaagt agagaaactg
    20461 cgtgaacgcc tggcgcgcat caacgcccgc gcaccggtct acaccgtcac ccacggtgac
    20521 atcgacctgg gcctgttgtt caacaccaac ggttttatgc tggaagaaaa cgtcgtcagc
    20581 accaaaccgc gtttccactt tatcgcggat aaacaaaacg atatttcgtc gattgtggtg
    20641 gaactggatt acccggtaga tatcagcgaa gtttcccgcg tgatggaaaa cctgctgctg
    20701 gaatcggcgg ataaactact gcgttacaaa gggatgctgt ggattgacgg cgaacctagc
    20761 cgcctgctgt tccagggcgt ccaacgcctc tacagcgccg actgggaccg cccgtggggc
    20821 gatgaaacac cgcatagtac aatggtgttt atcgggattc agctgccgga ggataagatt
    20881 cgggcggcgt ttgcggggtt gaggaagtaa ttttagatga tggccgccat gtggcggcct
    20941 gaattatttt ttattctgat cgcgcagcat cgcttcacgt aaaattgcgt tcaggcgagt
    21001 ttgatagcct ttacctggtc gcttcaacca ttccatcaca tcagcatcaa tacgcaccga
    21061 agcctgagtt ttcaatggcc gataaaactt accgcgttct gcgttagacc attgctcatc
    21121 gctggaaggt gggatatcac tgtaatcaat atcttcgtcg gatttattcg ccagcgcctt
    21181 cagttcggcc tcatgctgca catcgagtgt agaggaggtc cctcgtttat gtttaaccat
    21241 gctcatagcg attccgttcg ttgtggatca tcaaacacta acaccgcatc ttcaaagcgg
    21301 atgccatgtt tcacccgatt acctttggcc ttatttgcat cccattcaaa ctccattggc
    21361 atagttgcca tccctaattg tatatacaaa aatgtatgta caattattct gcaattatag
    21421 cgttgatgtt ggaaatagca tcacgtttcg aataatcaac catgaaattg acaatgtgtg
    21481 agttggactt gattcatcca atagaatttc tcgcctgaca tggcacacta aacacctcgc
    21541 tgtcgttaag ggattaccgt gaaaaactac aaagtcatta ccccgctttt tcctacctat
    21601 gcgcaagtta aggctatgat gaaagccgtc tcaggctatt ccctgaaagc ggtgcgcaat
    21661 atgattactg ccattcacga gcaaaccgga acgccgcaaa aacccgttga ctggtcggaa
    21721 cctgacctgt ggatcagcga acgcttaacg ggtgaagatg ccgacatcgc ccgtcgtatt
    21781 tgggataccg ataatcacat attgaacccg cgccacagct acggctgcta cttattcctc
    21841 aactatcccc agtttgagct gatggaaagc acgccagacg atacctggca gcccacctcg
    21901 cgcgggcaaa aattcctgca agatgacgaa aaaacgctgc gttcactgga cgatcaggaa
    21961 ggcattctgc aacttcttga actattagct ggccgcgaaa tgtcgcgccg cgccgattta
    22021 ttgccggaat ggcaggcctt tttgcatcag cattcgaaat tcgcgtctgc aagttcagtg
    22081 aaaagtacgc tctactcccg tctctataac cttatcgacc gtgacatggt tgaccgtgaa
    22141 ggcatgagct atcgcatcac cgataccggg cgcgcctggc ttggaaaagc gttgccggca
    22201 cgaaaaagcg atccacgtaa agagctgctg gaagcggtga aacgctacaa cgagcagcaa
    22261 aaagagctgc tgcgcgaaca aatcagcacg atgaacccgt acaaatttga acaactggtt
    22321 ggtcaattac tggaagcaat gggctacgag caggtcgaag tgaccaaagc ctctggcgac
    22381 aaaggtgtgg acgtgattgg ccaggtgcag gtcggcatca ccactattac cgaagtggtg
    22441 caggtcaaaa gaatgcagaa cactatcact cgcccatata tcgaccagtt gcgcggggcg
    22501 ttgccttacc acaaagccat tcgcggtaca ctcatcacca ccgggaaatt tgcggcgaaa
    22561 tgtgcagaag cggcgctctt tccgggtgcc gcgcccatta cgcttatcga cggcgatcgt
    22621 ttactggaac tgttaatcga aaataacgtc ggcattcgcc gcagcaatgc agtggaattg
    22681 ctggatgtgg atttgcagtt gtttgatgag ctggagatgg attaaagggc gaaatccgcc
    22741 gcctctacca ttacaatagc gacccaattt atctgaacag agttcatcat ggccaaagca
    22801 ccaactaaaa aagccaaagc aaagaaaggc tttgaagaaa cattatggga tacagcaaac
    22861 cagcttcgcg gtagcgttga gtcctccgaa tacaagcacg tggtgttgag cctcgtgttc
    22921 ctgaaattca tcagcgataa gtttgaagca cgccgcaaaa aaatgattgc cgatgggcaa
    22981 gcagacttcc ttgagatgga agtgttctac cagcaggaca acattttcta cctgccggaa
    23041 gaagcgcgct ggtcttttat caaacaaaat gccaaacagg acgatattgc ggtccgtatt
    23101 gacaccgccc tctcgaccat agagaaacgt aacccaacgc tgaaaggcgc gctgccagac
    23161 aactacttca gccgtcagaa tctggaaacc aaaaaactgg cgtcactgat tgataccatc
    23221 gacaacatcg aaacgctggc acacgaaact gacgttgaaa cgttatcgaa agaagacctg
    23281 gtcggacgcg tttacgaata cttcctcggt aagtttgccg caactgaagg caaaggcggt
    23341 ggtgagttct acacgcccaa atgtgtggtt acgctgttaa ctgaaatgct cgaacccttc
    23401 cagggcaaaa tttatgaccc gtgctgcggc tcggcaggga tgttcgtgca gtcggtgaag
    23461 tttgtcgaga gccatcaggg taaaagtcgt gatatcgcgc tgtatggtca ggaactgaca
    23521 gccacgacgt ataaactggc aaaaatgaac ctcgccattc gcggtctttc agctaacctc
    23581 ggcgaacgac cggcagacac cttctttagc gaccagcacc cggacctgaa agccgactac
    23641 attctggcga acccgccctt caacctgaaa gactggcgta atgaagccga attaaccaaa
    23701 gacccacgtt ttgctgggta tcgtatgccg ccaaccggta acgccaacta cggctggatt
    23761 ttgcatatgc tctccaagct gtcggctaac ggcacagcag gttttgtgct ggcaaacggt
    23821 tcgatgagtt ctaacaccag cggtgaaggc gagatccgtg cacagatgat cgaaaatgat
    23881 ctgattgact gtatgatcgc cctacctgga cagttgttct acaccacgca gatcccggtg
    23941 tgtttatggt ttatgaccaa atcgaaggct gccgatccgg ccaaaggtta tcgtgatcgt
    24001 cagggcgaga cgctgtttat tgatgcgcgt aacctcggca ccatgattag ccgcacaact
    24061 aaagagttaa cggcggaaga tattgccacc atcgccgata cctaccatgc atggcgcagt
    24121 acgccggaag aactggctgc acggattgcg cgtggtgaca gcaagctgga aaaatatgaa
    24181 gaccaggctg gcttctgcaa agttgcgacc ctgcaagaca ttaaagataa cgactacgtt
    24241 ctgacaccgg gccgctatgt aggtgcagcc gagcaggaag aggacggcgt ggcatttgag
    24301 acgaaaatgc gcgaattgtc gaagacgttg tttgagcaga tgaagcaggc ggaagaactg
    24361 gatcgtgcga ttcgccagaa tctggaggtg ctgggttatg gggagtaagt ggatagaagt
    24421 atcactgggc gagatttctg aaaaaattgg agatggaatc cacggaacgc caact
//
LOCUS       DAZGQL010000081        24446 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.081, whole genome shotgun sequence.
ACCESSION   DAZGQL010000081 DAZGQL010000000
VERSION     DAZGQL010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24446
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.081"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..234)
                     /locus_tag="ACHKIR_003628"
     CDS             complement(<1..234)
                     /locus_tag="ACHKIR_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFO9991309.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLPGHGGSAAISVDGFDDVTDLLRKTLVSYNIL"
     gene            complement(231..1901)
                     /gene="menD"
                     /locus_tag="ACHKIR_003629"
     CDS             complement(231..1901)
                     /gene="menD"
                     /locus_tag="ACHKIR_003629"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HFO9991310.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     EQHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPKNERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETALADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(1990..3285)
                     /gene="menF"
                     /locus_tag="ACHKIR_003630"
     CDS             complement(1990..3285)
                     /gene="menF"
                     /locus_tag="ACHKIR_003630"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HFO9991311.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQTFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIKAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGEIAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTALNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(3364..3669)
                     /gene="elaB"
                     /locus_tag="ACHKIR_003631"
     CDS             complement(3364..3669)
                     /gene="elaB"
                     /locus_tag="ACHKIR_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HFO9991312.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(3724..4185)
                     /gene="elaA"
                     /locus_tag="ACHKIR_003632"
     CDS             complement(3724..4185)
                     /gene="elaA"
                     /locus_tag="ACHKIR_003632"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO9991313.1"
                     /translation="MIDWQDLHHSDLSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     EGENRHILGWHNGTLVAYARILKSDDELQPVVIGRVIVSEALRGEKIGQQLMSKALES
                     CTRRWPEKPIYLGAQAHLQNFYGQFGFIPVTDIYEEDGIPHIGMAREVIQA"
     gene            4250..5167
                     /gene="rbn"
                     /locus_tag="ACHKIR_003633"
     CDS             4250..5167
                     /gene="rbn"
                     /locus_tag="ACHKIR_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HFO9991314.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPHGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEIFDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQALKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCNA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPVTELANDFTVFNV"
     gene            5355..5468
                     /locus_tag="ACHKIR_003634"
                     /pseudo
     CDS             5355..5468
                     /locus_tag="ACHKIR_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
     gene            5523..6494
                     /gene="yfbL"
                     /locus_tag="ACHKIR_003635"
     CDS             5523..6494
                     /gene="yfbL"
                     /locus_tag="ACHKIR_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001604680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HFO9991315.1"
                     /translation="MKKMTSLCFIPLLLSLTFIIFYQPWVRALPPTPRHASPEQLEKT
                     VRYLTQTVHPRSADNIDNLNRSAEYIKEVFISSGARVTAQDVPITGGPYKNIVADYGP
                     ADGPLIIIGAHYDSVSSYENDQLTYTPGADDNASGVAGLLELARLLQLQTPGIGVQLV
                     AYASEEPPFFRSDEMGSAVHAASLERPVKLMIALEMIGYYDSTPSSQDYPSPAMSWLY
                     PDRGDFIAVVGRMQDINAVRQVKAALLSSRDLSVYSMNAPGFIPGIDFSDHLNYWQHD
                     IPAVMITDTAFYRNKQYHLPGDIADRLNYQKMAQVVDGVTTLLYNSK"
     gene            6597..7100
                     /gene="yfbM"
                     /locus_tag="ACHKIR_003636"
     CDS             6597..7100
                     /gene="yfbM"
                     /locus_tag="ACHKIR_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HFO9991316.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTKKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPAKNDPLSRAVLGEHSLEDGIDGFLGLTWNQELAATIDRLEA
                     LDRSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(7167..8624)
                     /gene="nuoN"
                     /locus_tag="ACHKIR_003637"
     CDS             complement(7167..8624)
                     /gene="nuoN"
                     /locus_tag="ACHKIR_003637"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HFO9991317.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(8631..10160)
                     /gene="nuoM"
                     /locus_tag="ACHKIR_003638"
     CDS             complement(8631..10160)
                     /gene="nuoM"
                     /locus_tag="ACHKIR_003638"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HFO9991318.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(10324..12165)
                     /gene="nuoL"
                     /locus_tag="ACHKIR_003639"
     CDS             complement(10324..12165)
                     /gene="nuoL"
                     /locus_tag="ACHKIR_003639"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HFO9991319.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLGTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(12162..12464)
                     /gene="nuoK"
                     /locus_tag="ACHKIR_003640"
     CDS             complement(12162..12464)
                     /gene="nuoK"
                     /locus_tag="ACHKIR_003640"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HFO9991320.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(12461..13015)
                     /gene="nuoJ"
                     /locus_tag="ACHKIR_003641"
     CDS             complement(12461..13015)
                     /gene="nuoJ"
                     /locus_tag="ACHKIR_003641"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HFO9991321.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(13027..13569)
                     /gene="nuoI"
                     /locus_tag="ACHKIR_003642"
     CDS             complement(13027..13569)
                     /gene="nuoI"
                     /locus_tag="ACHKIR_003642"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HFO9991322.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(13584..14561)
                     /gene="nuoH"
                     /locus_tag="ACHKIR_003643"
     CDS             complement(13584..14561)
                     /gene="nuoH"
                     /locus_tag="ACHKIR_003643"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HFO9991323.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(14558..17284)
                     /gene="nuoG"
                     /locus_tag="ACHKIR_003644"
     CDS             complement(14558..17284)
                     /gene="nuoG"
                     /locus_tag="ACHKIR_003644"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HFO9991324.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSAE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(17337..18674)
                     /gene="nuoF"
                     /locus_tag="ACHKIR_003645"
     CDS             complement(17337..18674)
                     /gene="nuoF"
                     /locus_tag="ACHKIR_003645"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HFO9991325.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEVGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGAWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(18671..19171)
                     /gene="nuoE"
                     /locus_tag="ACHKIR_003646"
     CDS             complement(18671..19171)
                     /gene="nuoE"
                     /locus_tag="ACHKIR_003646"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HFO9991326.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(19174..20976)
                     /gene="nuoC"
                     /locus_tag="ACHKIR_003647"
     CDS             complement(19174..20976)
                     /gene="nuoC"
                     /locus_tag="ACHKIR_003647"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HFO9991327.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(21070..21732)
                     /gene="nuoB"
                     /locus_tag="ACHKIR_003648"
     CDS             complement(21070..21732)
                     /gene="nuoB"
                     /locus_tag="ACHKIR_003648"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HFO9991328.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(21748..22191)
                     /gene="nuoA"
                     /locus_tag="ACHKIR_003649"
     CDS             complement(21748..22191)
                     /gene="nuoA"
                     /locus_tag="ACHKIR_003649"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HFO9991329.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(22821..23759)
                     /gene="lrhA"
                     /locus_tag="ACHKIR_003650"
     CDS             complement(22821..23759)
                     /gene="lrhA"
                     /locus_tag="ACHKIR_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HFO9991330.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPST
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
BASE COUNT         6065 a         6735 c         6213 g         5433 t
ORIGIN      
        1 aaggatgttg taactaacta aggttttacg cagtaagtcg gtgacatcat caaatccatc
       61 gacgctaatc gccgccgaac caccgtgacc tgggagatca acatacaacc gtgagtagtc
      121 ggcaaacgcc tcgcccactt cttgccattc atggcaatcg ccggaaaaac cgtgcaaaaa
      181 caccagccag ggtaaacctg gtttaccgtg ttttgcctgc gcgtgcagga tcataaatgg
      241 cttacctgcg ccagcagttg ctggagggtt tgcgcaccat cggtgtcgtt aaccaccatt
      301 tcaattaccg tggtggtcgg cgtgcgccag gcgtcggcaa gtgccgtttc aagttcctgc
      361 cagttttgcg gacgatgata tttcagctcg aacatcgcgg cggcgtgctc aaaatggacg
      421 ttttgcggca tcagatagaa acgctcacgc tcgtttttcg gcgttggcaa cagcgagaaa
      481 atttgcccgc cgttgttgtt caccacaatt aataccagcg gcgcggaaac ctgacgtaat
      541 aacgccagcg cgttgagatc gtaaagtgct gagagatcgc ccacaatcgc cagcgtcggt
      601 ttgccactgg cccgctgaac gccagcggcg gtcgagagca gcccgtcgat accgctggca
      661 ccacggttgc tgtacaccgg gtaacctgcc ggaagttgcg aaagcgcatc aatcagacgt
      721 accaccaggc tgttaccaac aaacaattga ccctgttcag gcagatagtc gctgatgcga
      781 tgcgccagtt gcgcttcgcc aaacgcatcg cgacgggcaa taaccgcctg cattgcctgt
      841 tccgccaggc gcggaatttc aacgcaccag ggctggcgtt tttctgccgg atgctgctcc
      901 agccagtcgg caatattggc aattaagcga cgtccacgat ggtgtgccgg atcaagtcgc
      961 ccttcgatgt catcaacaat ccagtactct tccggttcac agcttgcctg ccattgcagg
     1021 agccgtttgc ccgtcaggct gcttcccagt tgcaccacaa tttgtgcctg ttgcagttcg
     1081 ctggtcgctt tggcattgcc taaccagaga tcggcacacg gcagcggctg tccggtttgc
     1141 gacagcacgt cgccaatcag cggccagcca agagtttgcg cccacagtgc aacttttttg
     1201 ccctcttccg cactcatgcg cccggcaacc accacgccgc gcttttgccg ccagaagaac
     1261 cagtcgcgct gtttttcact ttccagacga ggcgcttcac gcagccacgg tttgtcgtcc
     1321 tgccaccagt cgcccagccg ctgttgccag ctaagcccgg tgtcgtccat ttcgccatac
     1381 agcggttcag caaacgggca gttgatatgg actccgcccg catgaagcgt accgagagca
     1441 tggtcgatgg tagaaaccag ccaacgtgca ggaatatcct gagtcgggcg cggcagtgaa
     1501 atactgtgcg tggggtgaga ggcgaacatt cccggctgac gaatcgcctg attcgcgccg
     1561 cagtcaatta gctccggcgg gcgatcggcg gttaacagga tcagtttttc tccggttaac
     1621 ccagcttcaa tcagtgccgg atagagattt gctaccgccg tgccggaggt cacaatcacc
     1681 gccaccggct gcttgctgac tttcgccagc cccagcgcca aatgccccag tccacgctca
     1741 tcgaaatggg tgtggtgaat gaatgcggaa ttctccgccg ccgctaacgt taacggtgta
     1801 gaacgcgagc caggggcgat acagatgtgt ctgacgccgt gacgcgttaa tgcttccaga
     1861 atgaccgccg cccagcgtcg gttaaatgcg cttactgaca tgagtttgtc cggtatcaat
     1921 attgcggcta agtataagga gctacaaaaa tagattattg atatgaatcg gtaatgatgc
     1981 gactcattac tattccattt gtaataaagt acgcagccct gccgctttgt tatcgatttc
     2041 ctgccactct tgctcggggt cagaaccacg gacaatgccc gcgccagcat atagccgcac
     2101 gacattgcca ctgatttttg ctgagcgcag ggaaacgcag aattcgcttt gttgtaaaga
     2161 gagatagccc gcagaaccgg cgtaccattc tcgggtgaac ggttcgtgac gggcgataaa
     2221 ctgtcgcgcc agatcgcgcg gtaagccagc aactgctgcc gttggctgca actgatgtaa
     2281 acagatcaca tcatccgctt tgttgagtgc agtccagata cagcggcgaa gatgctgcac
     2341 tttacgcaga cgcagtacct gcggcggtaa aacatccaac gtctgggtat cggcctgcaa
     2401 tcgttggcag atatcctcca ccaccaacat gttctcgcgc tggtttttat catccgccat
     2461 cagccactca cctaactgct gcgcctgctt atcatcagga tgatttgcta ccgttcccgc
     2521 cagcgcttca gtacgcagcg ctttgtcacg ccgccgccat aaccgttccg gtgaagagcc
     2581 aagaaaagca atttcgccat caaaggccat gtaaaaatgg tagcaattca gattcagtcg
     2641 acgactggca gccatcatcg ccgccgcatt gaccggactt gcgaaatgca ggtcagttgc
     2701 ccgagcaagc accactttgt cgagctcacc ttcggcgatg gttttcgttg ccagttcgat
     2761 taattgcgtc cagcccgttt tgtccggcca gtgttgttct cgggtggtgg ttaaatgtaa
     2821 cccaggcaag ggtttaatac tcaccagtgt ggcgataaat tcttttgcct taatcgcatc
     2881 atgctgaagg gagctttcgc tgaatagcgt cagccgcagc gtggctttac cgccacagcg
     2941 tcgccattcg aggcgtggta aaagtaaatt gccctgcgac gggtcaaacg catttagccc
     3001 ccagatgcgt aagtcggcgt gttccgggtg ctgacgaaga aacgtttgtg cctggtccaa
     3061 cgacgtaaaa cgggtaatcg cgcccaggac ggcagcttct tcatcaccat tacgttgttg
     3121 ccagtagaat tgcgggtacg tttgctgact ggccagccag ctcaaggcat caaaagcgtc
     3181 tttgagaggg aaaggaatat caataacccg aatgccgggt gtcgccggaa tctcttgcga
     3241 caaatggcgc agtagatttt ccagcgccgt agtaagtgat tgcacgcgga cctctccctg
     3301 ttaaaagcct cacattatac ggggtacgac aaaaaaaatg cagtaccccg gtgtagggag
     3361 gttttaacgg cgtgccagca acagtcctag taccagccca acggccgcgc ccacaccaat
     3421 tccttgccag ggtttttcgt ggacgtagtc atcagcacga taaacagcct gcttcgcccg
     3481 atagtaataa ctgtctgaag cctggctaac ccgttttttc acatcatcca gcgctttttc
     3541 tgcacgcgct ttcagctcaa catatttctg atcggcggga tcgccagagg agcggagcac
     3601 ctcttccagt gtttcactaa gcagcgtcag gtcatcatca atacgtgtat caccaaactg
     3661 attagacatt ctcgttctcc atttgtgtaa aacctgtcac taactataga caagggttat
     3721 tgattacgcc tgaattacct cgcgtgccat cccaatgtgt gggataccat cttcttcata
     3781 aatatcggtc acagggatga aaccaaattg cccataaaag ttttgtaaat gcgcctgcgc
     3841 ccccagataa atcggcttct ccggccagcg gcgagtacac gattccagcg ccttactcat
     3901 caattgctga ccgatttttt caccgcgtaa ggcttcgctg acaatcaccc gaccaatcac
     3961 aacgggttgc agttcgtcgt cacttttcag aatcctcgca tacgccacca gtgtcccgtt
     4021 atgccaaccg agaatgtggc gattttctcc ctccagatcg tcaccatcga tatcctggta
     4081 aggacagttt tgttccacta caaataccgc acaacgcagt tgtaataagg cataaagctg
     4141 agaaacggat aaatcagaat gatgcagatc ttgccagtcg atcatggaag gctcctttgt
     4201 gctgtttgct ttatactagc cggaatattt tcagcagcgt gatgaactca tggaattaat
     4261 ttttttaggt acttcagccg gtgtgccaac ccgtacgcgc aatgtcacgg caatattact
     4321 taacctgcaa catccgaccc agagcggtct ttggttgttt gactgcggtg aaggcaccca
     4381 gcatcagcta ctgcataccg cctttaaccc tggaaaactg gacaagattt ttatcagtca
     4441 ccttcatggc gatcatctct ttggtttacc cggcttgctg tgcagtcgtt ctatgtcagg
     4501 cattatccaa cccttaacga tttatggtcc gcacggtatc cgtgaatttg tggagaccgc
     4561 gctgcggatt agcggctcat ggaccgatta tccgctggaa attgtcgaaa ttggcgctgg
     4621 cgaaattttc gatgatggcc tgcgcaaagt gaccgcttat ccgctggaac acccactgga
     4681 atgctatggc tatcgtattg aagaacatga taaaccgggt gcattaaatg ctcaggcatt
     4741 aaaagctgct ggcgtgccgc ctggcccact gtttcaggca ttaaaagcgg gcaaaacaat
     4801 cacgctggaa gacggaaggc agattaacgg cgcagattac ttagccgctc cagtgccagg
     4861 taaagcgctc gctattttcg gcgataccgg cccctgcaat gccgcacttg acctggctaa
     4921 aggtgtcgat gtcatggtgc acgaagcgac gctggatata accatggaag ccaaagccaa
     4981 tagtcgcggt catagctcta cacgccaggc tgcgacactg gcccgtgagg ctggagtcgg
     5041 taagctaatc attacccacg tcagctcgcg ctatgatgac aaagggtgtc agcacctgtt
     5101 acgtgaatgc aggtcaattt tcccggtgac tgaactagcg aatgatttca ccgtgtttaa
     5161 cgtttaactc tgcttattat taaaaacagg gcgaaacttg ccctgttatc gcaacccgcg
     5221 ctacatatat ccacccacac gggtatattg agcgattttt gatatttatt catctcctca
     5281 cgcactatat tcagagcaat aagttgcctc tttttatctt cattcagcac aacctttgca
     5341 actaaaagga gcctatgatg gttacagttg tcagcaatta ttgtcaatta tctcaaaccc
     5401 aactctgtca gacatttgca gaaaaattta ctgtgaccga ggaattactg cagtctttaa
     5461 aaaaacactt ataactcatt acaaataatg atgaaataac gctttttatt gataatttac
     5521 atatgaaaaa aatgacatct ctttgtttta taccattact actatcgctt acttttatta
     5581 ttttttacca accctgggtc agggcattac caccaacgcc ccgacacgct agtcctgagc
     5641 aattagaaaa aacagttcgt tatcttacac aaactgtgca tccacgtagc gccgacaata
     5701 ttgataatct gaataggtcc gcagagtata taaaagaggt ctttatcagt agcggtgcca
     5761 gagttaccgc gcaggatgtc cccattacgg gtggccccta caaaaacatt gttgctgatt
     5821 atggtcctgc cgatggaccg ctgattatta ttggtgcgca ctatgactct gtcagtagtt
     5881 atgaaaacga tcaattgacc tatacgccgg gcgcggatga taacgccagc ggcgtggcag
     5941 gattactcga actggcgcgt ttgttacagc tgcagactcc agggattggc gtccaactgg
     6001 tcgcctatgc gtcggaagaa ccgcccttct ttcgtagcga tgaaatgggg agcgcggtac
     6061 atgcagcttc gcttgagcgt ccagtgaaat taatgatagc gctggagatg attggttatt
     6121 acgactcaac gcctagtagc caggattacc catccccggc aatgtcctgg ctttatcccg
     6181 atcggggaga ctttattgcc gtggtcggca gaatgcagga tatcaacgcc gttcgtcagg
     6241 taaaagcggc attgttgtca tcccgggatt tatctgttta ttctatgaat gccccaggtt
     6301 ttattcccgg tattgatttc tctgatcacc ttaattattg gcaacacgat attcccgccg
     6361 taatgattac tgacaccgct ttttatcgta ataaacaata ccacttgccc ggtgatatcg
     6421 cagacagatt aaattatcag aaaatggctc aggtagtaga tggagttaca accttgttat
     6481 acaacagtaa ataataaaaa tttctcgagg cggatccaga aaaagcaaca tgcgcatttt
     6541 attttctttc gtctgtttta agatgcgaaa catgaaattc aacggaggaa aaataaatgg
     6601 gaatgattgg ctattttgca gaaattgact cagaaaagat taaccaactg cttgagtcta
     6661 ctaaaaaacc tttgatggat aatatacatg acacgctttc aggattacgc cgactggata
     6721 ttgataaacg ctgggatttt ttacactttg gcctgacggg tacatctgca tttgaccccg
     6781 cgaagaatga cccgctaagt cgcgcagtgc ttggtgaaca cagcctagaa gatggcatag
     6841 atggcttcct gggattgacc tggaatcagg agttagccgc gacaatcgac cgactggaag
     6901 ctctggatcg aagtgagctg cgtaaacagt tttccattaa gcgactcaat gaaatggaga
     6961 tttatccggg agtaaccttt tcagaagagt tagaggggca actctttgct tcgatcatgc
     7021 ttgatatgga aaagctgatt agcgcgtatc gacgtatgct gcgacaaggg aatcatgcgc
     7081 tgacggttat tgttgggtga tggtgtcagg gcaccaaaaa aaaccgccga acatgtcggc
     7141 ggctttctga cttacaaact gactgcttac atcagcggca ttgctaaccg cacaatgcta
     7201 atcagcggtt gtggccatac acccagcacc agtaccaaca gtgcagagat cagcaccacg
     7261 ataccgcctg cgctgtactg ccagtttgat ggtgcatcgc gacccggttg ttccggagcg
     7321 tgaaggtata agctcaccgc cacgcgcagg tagtagtaga ggccgattgc cgaaccgaca
     7381 actacggcac ccaccagcca ccacaagtgt gcctggacac cgaccgccag cacgtagaac
     7441 ttaccgataa agcccagcgt cattgggata ccagccagtg aaagcatcat caccgtcatc
     7501 actgccgcga ggatcggacg atgccagaac agaccgcggt aagagaacag ggaatcagca
     7561 tccgggccac gatacgggct ggacatcagg ctgaccacgc cgaacgcgcc gaggctgctg
     7621 aacagataac ctgccaggta aacccctacc gcttccatcg acatctcgcc agtttgcagc
     7681 gcaatcagcg ccaccagcag atagccgagg tgagagatag atgagtaacc gagcagacgt
     7741 ttgatattgg tctggctcag cgccatcagg ttaccgaaga tgatggaggc aaaggcgata
     7801 atcgccagca ccacgcgaat cgcttcgctg tcacccaccg gtgcgtagag gaacagacgc
     7861 atcaccacac cgaagatagc gattttgctc gccgtcgcca ggaaagtgga aaccggcgca
     7921 ggcgcgccct ggtatacgtc tggcgtccac aggtggaacg gcaccagaga gagtttgaag
     7981 ccgaggccga caatcatcag gccgaaacct gccagcaaca gcggctcgtt gagcataccg
     8041 tcgccgaggt ttttacccag cgcgacaaac gacaggtcgc cagactgcgc atacaccagc
     8101 gccataccga acagcaggaa agaagacgct gcggcagaga gaatggtgta tttgatactg
     8161 gcttccagcg aacgtttctg gcggaaagcg taaccgacca ggccaaacag cggcaaagag
     8221 atcagttcga taccgaggaa cagagacgcc agatggttgg cattcgccag caggatcccg
     8281 cccagggcgg caattaacac caacaggtag aactcgtcct tgttgtcgtt atagccttca
     8341 agccacgggt aggcgaaagt acaggtggcg aggctcgcca acaataccag cccggtgtaa
     8401 agcatggcga aaccatcaac gcgcatcagt ggcgtaacgt ccatagcgcc cgcctggccg
     8461 acaaaccaga gcgaaaccag cgccgcgtta agcccaataa ccgagagcgt agcgttgagg
     8521 aaatgattgc gtcgccacgc aatggagagc atcaccacca ccaccgtcaa gccgacgatc
     8581 agcaacggta gcagtgcgat caggttttgt ggagttattg tcatggcgat ttacggcctt
     8641 gtagtagtaa cggaattaac aaaccactgc tggatattgc caatcgcgga gtgcgaggta
     8701 tccagaatcg gctgcggata gaagcccagc agtaccagca gcaccaccag caacaggatc
     8761 ataaacagct cacgcagcga catccctggc agttcctggc tggcaatctg gcttttcgct
     8821 ttaccgaagt aagcgcgatg taacatcgcc agcgaataaa cagatgcaaa gaccagccca
     8881 aaggtagaga taacggtaat caccgggaca acctggaagc tgccgaacag aatcataaat
     8941 tcgccgacga agttaccggt gccaggcatc ccaagcgttg ccaccgcaaa gaacagcgac
     9001 agtgctggca gccatttcat cttgctccac agaccgccca tcatgcgcat gtcgcgggta
     9061 tggatacgtt cataaagctg accgcagaga ataaacagac ccgccgccga caagccgtgc
     9121 gcaatcatct ggattaccgc gccctggtag gccaactggc tgccagtgta gatagcaatc
     9181 agcacgaagc ccatgtggga aaccgaggtg taggcgatca gacgtttgat atcggtctgg
     9241 gcgaaggcca tccacgcacc gtagaagatg ccgataacac ccagccacat agcgattggc
     9301 gcgaactctg ccgacgcgtt cgggaacaac ggtagggaga aacgcagcag accgtaagcg
     9361 gcggttttca gcaagatccc cgcgaggtca acggaaccgg cggttggagc ctgtgagtgc
     9421 gcatccggca gccagccatg cagcggaacc accggcattt tcaccgcaaa tgcgatgaag
     9481 aagcccagca tcaacagata ttccacacca ctggacattg gcgtattcaa cagctcttca
     9541 tagttgaagg tccagacgcc ggtcgcattg tagtgaacaa aaaccagcgc caggatggcg
     9601 atcaacatca ccagaccact cgcctgggtg taaatgaaga acttggttgc cgccgtgata
     9661 cgcgttttac cgtcagaggc tttatgcccc cacagtgcga tcaggaagta catcggcacc
     9721 agcatcattt cccagaagaa gaagaacagg aacatgtcga tggcaaggaa cacgccgata
     9781 acgccgccca ggatccacat caggttgagg tggaagaagc cctgatattt ttcgatctct
     9841 ttccacgaac agagtaccgc cagcacaccg agcagaccgg tcagcacgac catcagcagc
     9901 gacagcccgt caatggcgag atggatagag ataccaaaac gcgggatcca cggcatgtcg
     9961 aattcagact gccactgcgg aattccggcg gattgcgtca gtgaataacc gccctgcaac
    10021 cacagttgca gcgacagcgc cagcgtcaat cccatggtga tcagcgcgat ccagcgcggc
    10081 accttgacgc caaagcgttc ggtctgccag cacaggaagc cgccgataaa gggaattaat
    10141 attagccagg gtagtaacat ggcgatcttt attccttgta aaagtcccgt caggaccgga
    10201 ttttcaacga attctcacga cagaaaattc tcttcggact gggggtagtg tcggatgcac
    10261 cgcgaatgcg gcttatccga cctacgttct tatatgccca ggggcaatcc cacaatcctt
    10321 aactcaacgc agtaccatca acagtgccag caccacgacc gcaccgatgc tcatggatgc
    10381 cacataccag cgcagataac cgttctcgct taacagcaga cctttacctg caaagcggga
    10441 aaggacagcc gggatgttca tcattgagtt cagcggatcg cgtttcagca accaggcaat
    10501 acccaggaac ggcttgacga acactttgtc gtacagccag tcaaagcccc aggcgttgta
    10561 ccaccaggta cccagcagac ggcccggcgc actgttggcg atggaggtca ccagagtacg
    10621 tttacccagc cacagccagg cagccagcag aatgccgacc accgcgacca cgccagaggt
    10681 aatttccagg gtcaacatgc tgccgtgcgc cagttccgtc gtttgcggaa gcacgccctg
    10741 cagcggcggt acaatcagtg cgccaacgaa ggtggaaagg atcagcagca caatcagcgg
    10801 caggctgtga gttacccctt tcacggcgtg agcgtgaatt tgttcttttc cgtggaagac
    10861 gatgaaaatc atacggaagg tgtagagcga ggtcataaac gcaccgacca gacctgccac
    10921 catcagattg atatgaccat tcgccatcgc acccgcgagg atctcatcct tactgaagaa
    10981 gcccgcagtg accagcggca gtgccgacag tgctgcgccg cccaccagga agcagagata
    11041 aaccagcgga atagatttac gcagaccgcc catcttgaag atgttctgtt cgtgatggca
    11101 ggccagaatg acggaaccgg atgccaggaa cagcagcgct ttaaagaacg cgtgggtcat
    11161 caagtggaaa atcgccgcat cccatgcctg cacgccaagc gcgaggaaca tgtagccaat
    11221 ctggctcatg gtagagtaag cgagaacacg tttgatatcg gtctgtacca gcgcggcaaa
    11281 accggccagc agcagcgtaa ccgccccgac aatgcccacc agatgcagaa cttccggcgt
    11341 catcaggaac aggccgtggg tacgagcgat caggtagaca cccgcggtta ccatcgttgc
    11401 ggcgtggatc agcgcggaga caggcgtcgg gcccgccatc gcgtcggcaa gccatgtctg
    11461 caacggcaac tgcgcagatt taccgaccgc accgcccagc agcatcagcg tcgcccacat
    11521 cagcatgtta ttgccgtcag caaagtgcgc tggtgccagt tccaccattt cgcggaagtt
    11581 cagggtgccc agttcgttgt aaagaatgaa cagcgcgaaa gcgaggaaca cgtcacccac
    11641 acgggtcacg acgaacgctt tcattgccgc tgcgccattc ttcggatcgg tgtaatagaa
    11701 accgatcagc agataggagc acaggcccac gccttcccag ccgaggtaca tcaacagtag
    11761 gttatcggcg agaaccagaa ctaccatgct ggcgatgaac aggttggtgt aagcgaagaa
    11821 gcgagagtag ccctcttctc cgcgcatata ccaggaggca tacatgtgaa taaggaaacc
    11881 cacaccagtg accactgaga gcatggtcag cgacaggccg tccagcacca gattgaaacc
    11941 aatgttaaag tcgcctaccg acatccacgt ccacagcggc tggctgtatg cctgctcacc
    12001 gttagcgaag aagtcaacgc cgataaaggc ggttaccagc gccgccaggc ccacagagcc
    12061 aacgccgacg atcgccgaga cgttttcaga ccagcgccca cgggagaacg ccagcaggac
    12121 gaagccaatc aatggcaaaa taatggttaa ggcaagcatg ttcatccgcg catctcactt
    12181 actgaatcga tgttcaggtt ctggcgacga cggtgaagtt gcagcagcag cgcaaggccg
    12241 atactcgctt ccgccgccgc gaggctgatg gcgagaatgt acatcacctg accgtcggtc
    12301 tggccccagt agcttccggc gaccacaaag gccagcgcag aggcgttaat catgatttcc
    12361 agaccaatca gcataaacag cagattgcga cggataacca gaccggttaa gccaagaacg
    12421 aataagattg ccgcgaggat cagtccatgt tgtaagggga tcatgcgtgc tcctccgttt
    12481 ttcttttcgc gctgtcgtct ttacgattgc tcagcacttc acccgcacgc tcttcacgac
    12541 cgacgtggaa ggccacaacc agacccgcga gcagcagcat agaagccagt tccaccgcca
    12601 gtacgtaagg cccgaacagc gtaataccca ctgctttagc actgattggc gtaccgtcga
    12661 taccctgatc attaacacca aggatggcgt aaacaatcac caccagcatg atggccgaca
    12721 aaatcgccgg accaatccac acctgcggtt tcagccactg gcgttcctgt tcgatttctg
    12781 aaccgcccag gttgagcatc atcaccacga acacgaacag caccataatg gcacccgcgt
    12841 agacgataat ttccagcgca ccggcgaagt aagcgcccag tgagaagaac accccagaaa
    12901 tcgccagcag cgaaataatc aggtacagca gcgcgtgtac cggattggta tgggtgatca
    12961 ctcgcaaggt cgcaagtatg gctatcaggc cacagatata aaaagcgaac tccattgccc
    13021 ctctccttac ggtaacaggc tcttgacgtc gataggcttg gcttcgttct ctgcttcgcc
    13081 cttatctttg ccgtcgattg ccatacctgc catccggtag aagttatatt ccgggtattt
    13141 gcccggaccg gagatcagca gatcctcttt ctcgtaaacc agatcctggc gcttgtattc
    13201 ccccatttcg aaatccgggg ttaactgaat cgccgtggtc ggacaggctt cttcgcacag
    13261 accacagaaa atgcagcgtg agaagttgat gcggaaaaat tccggatacc agcgaccgtc
    13321 tttggtttct gctttttgca gcgagataca gccgaccggg caggctaccg cgcagaggtt
    13381 acaggctacg caacgctctt cgccgtccgg atcgcgggtc agaacgatac gaccacgata
    13441 acggggcggc agatagaccg gctcttccgg gtacattcgc gtttcgcgtt tggcgaacgc
    13501 gtgcaggccg atcatccaga tactacgaac ctgggtgccg aaacctacta acaattcttt
    13561 taaggtcatg gtcttattgc cccttattgc gcctgccaga gaatgacagc cgccgttacc
    13621 agcaagttga tcagcgtcag cggcaggcag attttccagc cgaaggacat tacctggtca
    13681 taacgcggac gcggtaacga cgcacgaatc aaaatgaaca tcatcataaa gaacgcggtt
    13741 ttcagcgcga accagatgaa tggcggtaac aacgggcctt gccagccacc gaagaacagc
    13801 gtcaccatca gtgcagagat ggtcacaatc ccgatgtatt cgcccacgaa gaacagaccg
    13861 aacttcatgc cggaatattc aatgtggtaa ccatccgcca gttcctgctc ggcttccggc
    13921 tggtcaaacg ggtgacggtg acacaccgcc acgcccgcga tggcaaaggt aataaaacca
    13981 aagaattgcg ggatgacgtt ccacacatgc gcctggctgt tgacgatgtc ggtcatgttg
    14041 aatgaaccgg cctgcgccac cacgcccatc agggaaagcc cgaggaacac ttcgtagctc
    14101 agggtctgcg cagaagcacg catcgcaccc agcagcgagt atttgttgtt acttgaccag
    14161 cccgcaaaca gcaccgcgta aaccgccaga ccggccatca tcaggaagaa caaaatcccg
    14221 atgttcaggt cggcaaccac ccaacccgga ctgactggca caatcgcaaa ggccagcagc
    14281 agcgaggtaa aggcaatcat cggtgccagg gtaaagatga cgcgatccga gaatttcggg
    14341 atccagtctt ctttaaagaa catcttgatc atgtccgcga ccagctggag cgaaccgccc
    14401 cagccaacac ggttaggtcc gtaacggttc tggaacagac ccagcaggcg acgttcgcca
    14461 aagctcatga atgccccaca ggtgacaacc accagcagga tcaccaccgc tttgaggatg
    14521 gtcagcagga tctcaatcag ttccggtgat atccaactca ttgttgtgcc tccttgagat
    14581 cctcaagatg cgcgccagcc agcaccggag caatgccgga catacccatc ggcaagccca
    14641 cctgccctgc cgtcagtcct tcggcgattt caaccggcag cgtgaccgtg ttgccatcgt
    14701 aactaaagga gacgcgtgta cccgcgttca cacccaactt cgcggcatcc gctgggttga
    14761 gtttgatgta cggctgcggc atacggctct ggaagaccgg agcacgctgt gacaattcat
    14821 cgctaccaaa caggtggtag tacggcgcga tacgccattt cccgtcctgc ggctggaagc
    14881 gtgccggtac gctggtgaag taatccagac cattttcgct ggtttcaaac agacgcacgc
    14941 ccggatcgcc aaagcgcagt ttgccgccca cttcgtcctg gaatttgttc cacgcctgcg
    15001 gggagttcca gcctggcgcc caggcaaacg gcacttgcga acggtgcgcg gtcggctggt
    15061 tgttaccttc catcgagaag gtgaacatgg tgtcaatatc ctgcggctga cgcggctcat
    15121 gaacgctgat attggcgcgc atggcggtac gaccgctgta acggtgcggt tcacgggcca
    15181 gtttctgccc acgaatacgg aatgtcgcat ccggcgcagc atctttgata cccgccagtt
    15241 ccgggatttt cgccacaaca gcgtcaatca catggtcgag ctgcgtccag tccacttcac
    15301 ggctcagcaa ggtgctgtgc agcgagtgta accaacgcca gctttccagc atgacagttt
    15361 tgctgtcgta ataagccgga tcgtaaacct ggaagaaacg ttgggcacgg ccttcgttgt
    15421 taatcaccgt accgtcgctt tcagcaaagc tggcagcaga aagtaccaga tgggcgtttt
    15481 ccataatcgc tgtgcgttga tggtcaacca ccatcaccag cggcgcttta gccagcgcag
    15541 cattcacgcg ggtagcggaa gcgtgacgat gcagatcgtt ttccagcacc accaccgcgt
    15601 cggcgcgtcc ggtttccagt tcggttaacg cttcttcaag cgatccaccg cccataatgc
    15661 ccagccccat gctgttgacg gaacgggcaa tcatggtgat accgacgtca gcgccgcgac
    15721 ctttcagggc tttcgccacg ttagccgcag cctgaatcac ttcagcgcta ccggcgttcg
    15781 tcccggagat aatcaacggt ttcttcgcac ctgccagtgc ctgcacgatg acgtcgattt
    15841 tgctttgcag ctcaggttca ataccgtcaa ccgctggcgc ggagttatcc agcgcatggg
    15901 cgatggcaaa acctaaacgc gcctgatctt caaccggtgc gcggtaagtc cacgccgcga
    15961 tatcatccag acgggtgtca tcaacgttgg taacaaacag cggatgcttc gcacgttggc
    16021 cgatgttgag gattgccgca atctgccagt cagccacttt ctgtgctgcc gccatttcgc
    16081 gcgctttacc tttcaccgcc tgacgaactg ccagcgcgac gcgcgcgccg gtctgggtaa
    16141 cgtcttcgcc cagcaccagt accgcatcgt aagattcgat ttcgcgcagt gccggcgtat
    16201 aaatgccgcc ttcacgcagc actttcagcg ccagttgcag acgttcctgc tcaccgtgag
    16261 cgataccggt gtagaagttt tcttcgccca ccagctcacg cagcgcaaag ttgctttcca
    16321 cgctggcacg cggagaacca atgccgatca ctttcttcga ctgacgcaga atatctgccg
    16381 cgccctgcat tgcctgttcg gcgttgaggg taatgaaatc atcgccacga cgctgtactg
    16441 gctgacgcgg acgatctttc aggttgacgt aaccgtaacc gaaacgacca cggtcgcaga
    16501 ggaagtagtg gtttaccgta ccgttgtaac ggttttcgat acgacgcagt tcgccgtaac
    16561 gctcgccggg gctgatgtta cagccgatgg aacattgctg gcagatgctc ggcgcaaact
    16621 gcatatccca tttacggttg taacgctcgg agtgcgtttt gtcggtgaat acgccggtcg
    16681 ggcaaatctc gaccagatta ccggaaaatt cgctttccag cgtgccgtct tccgggcgac
    16741 cgaagtagac gttgtcgtgc gcaccgtaaa cgcccaggtc cgtgccgtca gcgtaatctt
    16801 tgtagtaacg cacgcagcgg tagcaggcga tgcagcggtt catttcgtga gagatgaatg
    16861 gccccaaatc ctgattacgg tgggtacgtt tggtgaaacg gtagcgacgg aagctgtgtc
    16921 cagtcatcac agtcatatcc tgaagatggc agttaccgcc ctcttcacat accggacagt
    16981 cgtgcgggtg gttggtcatt aaccactcga ccacactttc acggaactgt ttcgcttctt
    17041 catcgtcaat ggaaataaag gtgccatcgg aagccggtgt catacaggac atcaccaggc
    17101 gaccacgcgt gtcttccgcg ttttggtatt gcttcaccgc acactggcgg caagcaccga
    17161 cgcttcccag cgccggatgc cagcaaaagt aaggaatatc aaggcccaga gacagacaag
    17221 cttccagcaa gttgtccgct ccgttgacct cgtattcttt gccgtctaca tgaattgtag
    17281 ccattagcat gcttccagtt ttctcagtca gagactgagc gttaatcgaa attcggttac
    17341 cagcgctctt tcagcaggtt cggctgaatc ccattaatca aatgggtatt gctgaacggc
    17401 tgtttgattc ccgcctcaaa ttcttcgcgg aaatatttga tggcgctctg taacggctcc
    17461 actgcaccag gtgcgtgggc acagaaagtt ttacccgggc ctaagaatcg acacagttgc
    17521 tcaagtgttt cgatatcgcc cggctgacct tcgccacgct ccagcgcacg cagaattttc
    17581 acgctccacg gcagaccgtc gcggcacggc gtacaccagc cacaggactc acgggcgaaa
    17641 aactcttcca ggttacgcac cagcgacacc atgttgatct catggtcaac cgccatcgcc
    17701 agcgccgtac ccagacggct tcccgcttta ccgatacttt cgaattccat cggcagatca
    17761 aggtgcgctt cggtcaggaa gtcggtgcca gcgccgcctg gctgccaggc tttgaatttc
    17821 agaccgtcac gcataccacc ggcgtaatct tcgaggatct cgcgtgcggt ggtgccgaat
    17881 ggcagttccc acaggcccgg atttttcacc cgaccggaga agcccatcag cttggtgcca
    17941 gcatctttac ttttcgagat gttctgatac cactccacgc cgttagcgag gatcgccgga
    18001 acgttacaca gggtttcgac gttattgaca caggtcggtt tgccccatgc accggaggtt
    18061 gccgggaatg gaggcttcga gcgagggtta gcgcgacgcc cttccaggga gttgattaac
    18121 gctgtttctt caccgcagat gtagcgccca gccccggtat ggacgaacaa ttcgaagtcg
    18181 aaacctgttc ccataatgtt tttgccaagc agacccactt cggtggcttc ggcaatggca
    18241 cggcgcagat tcactgccgc ttcgatatac tcgccacgca ggaagatgta gccacggtac
    18301 gctttcagcg caaacgcgga gatgagcata ccttccacca gcaggtgcgg cagttgttcc
    18361 atcagcaggc ggtctttata ggtgcccggc tccatttcat cggcgttaca cagcaggtaa
    18421 cggatgttca tggattcgtc tttcggcatc aggctccatt tcaggccggt agaaaagccc
    18481 gcgccgccgc gccctttcag accagcgtct tttacctgat taacgatttc gtccggagac
    18541 agcccggtca gcgccttacg cgcgccttcg taaccgtttt tgctgcgata ttcgtccagc
    18601 cacactggct gtttgtcatc gcgcagacgc caggtcagcg gatgcgtttc gggagtacgg
    18661 ataatgtttt tcatttatac cgctccagca gttcagggat cgcttccggg gtcagatgcg
    18721 cgtgagtgtc ctcatcgatc atcatgtttg gccctttgtc gcagttcccc aggcagcaag
    18781 ttggcagcag cgtaaagcgg ccatcaaatg tcgtttgccc tggtttgatg ttcagctttt
    18841 tctccagcgc cgcctgaata ccctggtaac cgttgatgtg acagaccacg ctgtcgcaat
    18901 aacggatcac atggcgacca accggctggc ggaagatctg gctatagaac gttgccacgc
    18961 cttcgacgtc gctcgccgga atacccagca catcggcgat cgcgtggatc gcgccatccg
    19021 gcacccagcc acgctgcttc tgaacgattt tcagcgcttc aatggacgcc gcacgcgggt
    19081 cttcgtagtg gtgcatctcg tgctcaatcg cttcacgctc tgccgcactc agctcaaaag
    19141 cctcggtttg tggttgttga ttctcgtgca taattagcgg tccacatctg acataacaaa
    19201 atcgatactg cccagataaa caatcaggtc agacaccagg ctgccgcgga tcgccgccgg
    19261 aatttgctgc aaatgcgcat aactcggcgt acggatacgg gtgcgataac tcatggtgct
    19321 accgtcgctg gtcaggtagt aactgttgat ccccttggtc gcctcaatca tctggaaaga
    19381 ttcattggct ggcatcaccg gaccccacga cacttgcagg aagtgggtga tcagggtttc
    19441 gatatgttgc agcgtgcgct ctttcggcgg cggcgtggtc agcggatgat ccgctttgaa
    19501 cgggccttcc ggcatgttgt tgaggcactg ctcaagaatg cgcagactct ggcgcagctc
    19561 ttccactttc agcattacgc gggtgtagca gtcagaaaca ccaccaccca ccgggatttc
    19621 aaagtcgaag ttttcatagc cagaataagg acgcgcctta cgcacgtcga agtcgatccc
    19681 ggtggcgcgc aggcccgcgc cagtggtgcc ccactccagc gcctctttcg cgccataggc
    19741 ggcaacgccc tgggaacgac ctttcagaat ggtgttttgc agcgccgctt tctcgtaaga
    19801 cgccagacgt ttcggcatcc agtcgaggaa ctcacgcagc aggcgatccc agccacgcgg
    19861 caggtcgtgc gctacaccgc caatacggaa ccacgccggg tgcatacgaa aacccgtgat
    19921 cgcttccacc agatcgtaaa ttttctgacg atcggtaaag gcgaagaaca ctggcgtcat
    19981 cgcgccgacg tcctgaataa aggtggagat atacagcagg tgactgttga tgcggaacag
    20041 ttcggagagc attacgcgaa tgacgttaac gcgatccggc acggtgatcc cggccagttt
    20101 ctctaccgcc agcacgtaag gcatttcgtt aacgcagccg ccgaggtatt cgatacggtc
    20161 ggtatacgga atgtagctgt gccaggactg gcgttcgccc attttctccg caccacggtg
    20221 gtggtagccg atgtctggta cgcagtcgac aatttcttcg ccatcaagct gcaaaacgat
    20281 gcggaaagcc ccgtgcgccg acgggtggtt cgggccgagg ttgaggaaca tgaagtcctc
    20341 gttttcggtg ccgcgcttca tcccccactc ttccggtttg aaggtcaggg cttccatctc
    20401 caggtcctgt ttggctttgg tcagctcaaa cggcgagaat tcggtagcgc gcgccggata
    20461 atctttacgc agcgggtgac ctttccaggt ttgcggcatc atgatgcggc gcaggttcgg
    20521 gtgaccgtcg aaagtaatgc caaacagatc ccaggtttca cgctcatacc agttagcgtt
    20581 cgggaacagt ttggtgaagg tcggtacgtg caggtcgttt tctgccagcg ccaccttcag
    20641 catgatgtcg cggttacgat cgatagaaat cagatggtag aaaacggaaa aatccgcggc
    20701 aggtaaccct tcgcggtgtg tgcgcagacg ttcgtccatg ccgtgtaagt caaacagcat
    20761 gacgtaaggt ttcggcagtt tctttaagaa atcgcccact tccagtaatt gttcacgctt
    20821 gatccacaca acgggaaccc cggtgcgagt cgcctgaaca gtaaaggcat ccggcccaaa
    20881 acggttgcgc agttcgccaa tcaccggatc atcaagatga tcgcgggtct gccaggcggg
    20941 ttcttgcgcg gttaagtcgg tcatattgtt caccattgca aatggtccgt ggtgactgtc
    21001 ggcgcagtaa gcttcgcgaa atagatttag gaataagcga agaaaaattc ctttgccgac
    21061 aggcgcaaat taaatctcgt caggtgtacg caggttggtt acggcaatgc gttcaccgcg
    21121 cttgcgttcg cgctctgatt gcatattggc gcgataaacg ccctgatcgc caaccaccca
    21181 ggagagcgga cgacgttctt taccgataga ttcctgcaac agcatcagtg cctgcatgta
    21241 cgcttcagga cgcggagggc agcccgggat atacacatca accgggatga atttatcgac
    21301 gccctgcaca acggaataaa tatcgtacat accgccggag ttggcacagg cacccattga
    21361 gataacccat tttggttcca gcatctggtc atacagacgc tgaataaccg gtgccatttt
    21421 tgtaaagcag gttcctgcaa cgaccatcag gtcagcctga cgcggcgaag ctcgcaatac
    21481 ttctgcgcca aaacgcgcca cgtcatgcac cgcggtaaac gaagtcacca tctcaacgta
    21541 acagcaggaa agaccgaagt tatacggcca aattgagttt ttacgacccc agttaaccat
    21601 gtcattgagc ttgcccataa acacgttttt attaacttct tgctccagag ggtcggttac
    21661 gatctcctgc ttttgcaggg ggtaacggtc gttctcaccg ttgggatcta tgcgggtgag
    21721 cgtataatcc atcttaatgc ctcgcggtta gcgttgacga ttagcgatac tgttcgtttc
    21781 cgggttcata cgctcgcggc gtgaacgcgc gggcgtccag tccagcgcgc caatacgcac
    21841 cagataaacc agacctgcca gtaacacaaa aataaaaatt gcagcttcca caaagcctac
    21901 ccagccgctt tcgcggatag aggttgacca tgcgaacaga tacagcgctt caacgtcgaa
    21961 gataacgaag aacatggcca ccagataaaa cttggcggac aggcgtaagc gggcggagcc
    22021 gaccgagtcg ataccggatt caaacggaac gtttttcgac cttgcgcgtg cgcgaccgcc
    22081 taaaaaccaa ccgcctacca gcatcaggca acacaggcca atggcaacga taagaaagat
    22141 agcgaatgcc cagtgatgag cgatgacttc agtggatgtt gacatactca ttgcttactc
    22201 atcaaaagta gcgccagatt cactgctctt cacggcagat ggacgccaca tcgattcatg
    22261 gggaggaata aaaaaaacct tacaatcact gtagaaattc ttttatacag ctaattgatg
    22321 tggtctttta ctcctttcta taaccttttg tcaactttaa caaaagtttc ttcacattag
    22381 tttacataat atcaacacca ttagcattta atgccctttc accccagatc cttgacgact
    22441 ccaggataat tagatgttgt tgaatcgtgt ccgttgtgaa gcaatggaaa aaatacgggt
    22501 ctattttgac aggaatttgt gtcgattcct ccccccaaaa gagagtattt tcttgatctg
    22561 tgacacgctt ttgtcattcc ataacaaaaa cgcagcaaca aatttacgta ttttttaaca
    22621 tcattgtagc aggtgatttt tttcaggcga ttatttgtgc gttcgggacg tgaatctctg
    22681 gtgattgaaa aatgaacagt tttgtacgtt ctgcacgata cgaggaaggc tttttactaa
    22741 aaaaaagccg ctggggttta aaacaccccc agcggctcgt ttttacacta ttgtctcaga
    22801 aattatctat ggtccgtcga ttactcgata tccctttcaa tcaacaatga gtcatcgcct
    22861 tccggagcag acattgggct gtattgccac ggattatggt agctctccat cgcctgatag
    22921 atcacctggg ccagctcatt attactggac gggtcatagc acagcaaata ttcggtatcg
    22981 ggtagcggcg gtaagccatc tactccactc agcacacgca ggtctggact catcatctcg
    23041 accggccttg ccgtcacgcc aagaccggct tttactgccg cacgaactgc cggaagcgtc
    23101 gaggcgacat aagccagtcg ccatggaata tctgctttat taagcgtcgc cagcaccata
    23161 tcgcgaaacg ggctaggatc atccagcaat acaagaggga tcggctcgcc tttttgcaat
    23221 acgtattccg ctgcgcagta ccagtgtgtt ggcgaggtac gcaggttcaa tgccttaaac
    23281 gttgaaggac gatgggtggt taccatcaaa tccacttcct gggattccag catttctgcc
    23341 atataggcat tacgtttaac gcggacatcc agcgctagtt tcggataaac cgaactcacg
    23401 cgatttaaca ggaaaggtaa gatcgtatcg gcagattcat ctgaagcacc gatagttaac
    23461 acgccctgaa gattactgaa cattaatgat gagcaggcct catcattaaa acgcagaatt
    23521 tttctggcgt aaccaagaag ttgaatgcca tgttcagtta acagtttgtt gcgaccgtga
    23581 cgagcgaaca gttctttccc aacgagttgt tccagacgct gcatttgctg acttacggcg
    23641 gactgagtac gacacacagc ggcagctgcg gcagcaaaag tgttcagatc ggcaacagca
    23701 acaaatgttc tcagcagatc gaggtcgagg ttaattatcg gacgatttgc acttatcata
    23761 tattatcact tactggcggc tcatactgag ctggttaatg ctgtgcacac acaaacaagc
    23821 aattccattt gtaatgtgcc tccctggcag tttcatccgg aacccggacg aaagtaaaaa
    23881 tgcatatgag ttgcactaaa aaagcgactc acattgttcc gttataatgc ctgaagtaga
    23941 tcacagaata tatcttcagg aatcgcatat ctattaagtt actcactctt ttgtatttat
    24001 gacatgcgcg tgtttgtata aatgtaaatg tgagtccttg ttccactctc gtgcagcatc
    24061 gctggtcata cgcgaacacg tacaaacagc aatggtgagg cggcatcaag agcagggatc
    24121 cgtcatttat gcgagcattt taccccaaaa accttttatt tataagggtc attgcgaatt
    24181 atctgatgca aagttatgtt atgttaggca aagtaatctt ctattattaa taagcacaac
    24241 aaaacctttt tgaatattaa ataataatta attagcatca tcctcataca ttaatcccgc
    24301 ttaacaatag tttcacacct tgcacccatt acgacacctc ggaaatcatc acgctgttaa
    24361 actctccgta atgcggaaat atcatcatca cgattcatta gtgaattctt aaacgaggca
    24421 ttttcaaaca attatcttac agataa
//
LOCUS       DAZGQL010000082        23765 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.082, whole genome shotgun sequence.
ACCESSION   DAZGQL010000082 DAZGQL010000000
VERSION     DAZGQL010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23765
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.082"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..413)
                     /locus_tag="ACHKIR_003651"
     CDS             complement(<1..413)
                     /locus_tag="ACHKIR_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFO9991331.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDLALPGPLPFILSRTYSSYRTKTPAPVGSLGPGWKMPADI
                     RLQLRDNTLILSDNGGRSLYFEHLFPGEDGYSRSES"
     gene            642..1250
                     /locus_tag="ACHKIR_003652"
     CDS             642..1250
                     /locus_tag="ACHKIR_003652"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HFO9991332.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            1348..2739
                     /gene="selA"
                     /locus_tag="ACHKIR_003653"
     CDS             1348..2739
                     /gene="selA"
                     /locus_tag="ACHKIR_003653"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HFO9991333.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRDSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            2736..4580
                     /gene="selB"
                     /locus_tag="ACHKIR_003654"
     CDS             2736..4580
                     /gene="selB"
                     /locus_tag="ACHKIR_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HFO9991334.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETAHAGQRIALNIAGDAEKEQINRGDWLLADAPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
     gene            4771..5922
                     /gene="yiaY"
                     /locus_tag="ACHKIR_003655"
     CDS             4771..5922
                     /gene="yiaY"
                     /locus_tag="ACHKIR_003655"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HFO9991335.1"
                     /translation="MAASTFFIPSVNVIGADSLNDAMNMMADYGFTRTLIVTDNMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAEGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHIK
                     MAIVDKHVTPLLSVNDSSLMVGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     NIPAGLRDLNVKEEDIPVLATNALKDACGFTNPIQATHEEIMAIYRAAM"
     gene            6052..7347
                     /locus_tag="ACHKIR_003656"
     CDS             6052..7347
                     /locus_tag="ACHKIR_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFO9991336.1"
                     /translation="MAIKSPPGLIPLSHLSGEELLAHLRFNRVTDEKGRYLPFDELQY
                     RIKKGENVDVAWTLTRLARNAAIQRINYCNEAGEQAGFNITPVIAEACELVDKHATAL
                     ALKDQTERLRGAGAELSQLRLEEPITSSQLEGANTTTLVARKMLETGRSPRTEDEHMI
                     AGNARLMAEIPHLLAEPLTPALIRQLHAIGMGGINDAKYRPGEFRETDDVVIADYDGN
                     IVHQPPAAALLPERLEKVCQWLNSHEGYIHPLVRACILHFMLAHEHPFRDGNGRTSRA
                     LFYWYMLKSGYDVFKYISISRLLHAAPVKYAASYQYTESDGMDLTYFLEYQAGVIKRA
                     LQNWQQHIDEITQRSAKLDSVLFSSGVLKRLNPRQVTLLNVMLANPGKEYTVAEISAS
                     LGVSDNTARADLRTIVKEGFAQEKRINNQQAVYVAHYPL"
     gene            7455..8993
                     /gene="aldB"
                     /locus_tag="ACHKIR_003657"
     CDS             7455..8993
                     /gene="aldB"
                     /locus_tag="ACHKIR_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HFO9991337.1"
                     /translation="MTNNPPSAQIKPGEYGFPLKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(9212..9355)
                     /locus_tag="ACHKIR_003658"
     CDS             complement(9212..9355)
                     /locus_tag="ACHKIR_003658"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9991338.1"
                     /translation="MNNSSKTSTVQIKRIKPSIIYRLLLIGLGTPMVIYGLVRPLTIE
                     TRD"
     gene            9538..9861
                     /locus_tag="ACHKIR_003659"
     CDS             9538..9861
                     /locus_tag="ACHKIR_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFO9991339.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDVPYLIAKKRNHPHADAI
                     HVAGWVSLFTLHVIWPFLWIWATLYRPERGWGMQNHDSSVVQLQQRIAGLEKQLADIK
                     SSSAE"
     gene            9867..11003
                     /locus_tag="ACHKIR_003660"
     CDS             9867..11003
                     /locus_tag="ACHKIR_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFO9991340.1"
                     /translation="MDLLIILTYVAFAWAMFKIFKIPVNKWTIPTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQAR
                     VDRLMADIVTAEHKQRALGAELDEMAANTQQAKATRDKFAKEYQRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVKSAAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNLE
                     QTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGD
                     DAEVVFNALPGKVFSGKLAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELDEHT
                     DLSALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWVHYLYLDH"
     gene            complement(11000..11974)
                     /locus_tag="ACHKIR_003661"
     CDS             complement(11000..11974)
                     /locus_tag="ACHKIR_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO9991341.1"
                     /translation="MTKLQLKYRELKIISVIAASENISHAATVLGIAQANVSKYLADF
                     ESKVGLKVFDRTTRQLMLTPFGTVLLPYINDMLDRNEQLNNFIADYKHEKRGRVTIYA
                     PTGIITYLSKHVIDKIKDIGDITLSLKTCNLERNAFYEGVEFPDDCDVLISYAQPKDE
                     SLVASFITQYAVTAYASQRYLEKHPISRPDELEHHSCILIDSMMIDDANIWRFNVAGS
                     KEVRDYRVKGNYVCDNTQSALELARNHLGIVFAPDKSVQSDLQDGTLVPCFQHPYEWW
                     LDLVAIFRKREYQPWRVQYVLDEMLREIRHQLAQSQQLRPEQAAESED"
     gene            12098..12838
                     /locus_tag="ACHKIR_003662"
     CDS             12098..12838
                     /locus_tag="ACHKIR_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0030674 - protein-macromolecule adaptor
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HFO9991342.1"
                     /translation="MLINRNIVALFALPFMASATASELSIGAGAAYNESPYRGYNENT
                     KAIPLISYEGDTFYVRQTTLGFILSQSEKNELSLTASWMPLEFDPTDNDDYAMQQLDK
                     RDSTAMAGVAWYHHERWGTVKASAAADVLDNSNGWVGELSVFHKMQIGRLSLTPALGV
                     LYYDENFSDYYYGISESESRRSGLASYSAQDAWVPYVSLTAKYPIGEHVVLMASAGYS
                     ELPEEITDSPMIDRNESFTFVTGVSWRF"
     gene            complement(12959..14938)
                     /locus_tag="ACHKIR_003663"
     CDS             complement(12959..14938)
                     /locus_tag="ACHKIR_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003581012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HFO9991343.1"
                     /translation="MNISEVDLHKLTVSDPFLGQYQQLVRDVVIPYQWDALNDRIPEA
                     EPSHAIENFRIAAGLQDGEFYGMVFQDSDVAKWLEAVAWSLCQKPDAELEKTADEVIE
                     LIASAQCEDGYLNAYFTVKAPEERWSNLAECHELYCAGHLIEAGVAFFQATGKRRLLE
                     VVCRLADHIDSVFGPGESKLHGYPGHPEIELALMRLYEVTEEPRYLALTNYFVEQRGA
                     QPHYYDQEYEKRGQTSHWHTYGPAWMVKDKAYSQAHLPIAQQQTAIGHTVRFVYLMTG
                     VAHLARLSHDESKRQDCLRLWNNMAQRQLYITGGIGSQSSGEAFSSDYDLPNDTVYAE
                     SCASIGLMMFARRMLEMEGDSQYADVMERALYNTVLGGMALDGKHFFYVNPLEVHPKT
                     LKFNHIYDHVKPIRQRWFGCACCPPNIARVLTSIGHYLYTPREDALYINIYAGNSMEV
                     PVENGTLRLRVSGNYPWQEQVTIAVESPQPVRHTLALRLPDWCTQPQIILNGEEVEQD
                     IRKGYLHITREWQEGDTLNLTLPMPVRRVYGNPLVRHVAGKVAIQRGPLVYCLEQADN
                     GESLHNLWLPADAPFTTFEGKGLFRHKILIQAPGYRYEQSNPEQQPLWHYDSAPAKRQ
                     PQTLTFIPWFSWANRGEGEMRIWVNEEKHCHPEVG"
     gene            complement(14949..16349)
                     /locus_tag="ACHKIR_003664"
     CDS             complement(14949..16349)
                     /locus_tag="ACHKIR_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9991344.1"
                     /translation="MTSTPITAADIAKGKIDDALSVREKIGYGLGDAGGTVITCLIMN
                     FLTFFYTDVFGLTPALVGTLFIALRVFDAISDPVMGVIADRTQSRWGRFRPWQLWIAI
                     PIGIIGILTFTVPDASMGVKIAWAFGTYLLLSVGYTAINVPYCALINTMTTRHNEVIS
                     CQSWRFVLCGVAGFLVSVGLPWMVDLFGQGNAARGYQLGVGVLCAIAVVMFLCCFFWV
                     RERVPLSTMGKFTLREHLAGLRNNDQLLLMLVMSFLLINVFNIRGGGYMYFITYVLQG
                     STGYTSLFFTMVTFASIIGSVIVSPLTRRFDTVKIYYYTNLLLAALAVLMWFLPSGPA
                     YQTLWLAVILGNGVILGFTLPLHFSLMAFADDYGEWKTRVRSSGMNFAFNLFFIKLAW
                     ASSAGIISLLFIFVAYQPGVENQTTSSLGGITAMETLLPALFHLLLAGAIRFCKLNNP
                     MMSRIASDLRQRHVQP"
     gene            16575..17390
                     /locus_tag="ACHKIR_003665"
     CDS             16575..17390
                     /locus_tag="ACHKIR_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO9991345.1"
                     /translation="MLELSMTLPIKVQNGGLFISRGVGRHPARRLESWEIIFVEKGRL
                     TIQEEERIFLVDAGESLLLWPNRQHVGVEDFPADLKFYWLHFEVKAPDSDPRWLTHLS
                     VPQQTKVADPQALVALFRQFLSEQEKHQRTPALEFIVLLILQQLTRDARLDEQADAAG
                     TTLAWKAQKLIRTQYHRPLSTALLARELYCNADYLGRVYRRVFHLTLTEAIHRQRVRE
                     AEKLLISEARSLTEVAALCGFNDVGYFRQIFRKHTGLTPAAWKRRYSKEHINS"
     gene            complement(17422..18117)
                     /gene="araD"
                     /locus_tag="ACHKIR_003666"
     CDS             complement(17422..18117)
                     /gene="araD"
                     /locus_tag="ACHKIR_003666"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFO9991346.1"
                     /translation="MLEQLKADVLAANLALPAHHLVTFTWGNVSAVDEARQWMVIKPS
                     GVEYDVMTADDMVVVEIASGKVVEGSKKPSSDTPTHLALYRRYAEIGGIVHTHSRHAT
                     IWSQAGLDLPAWGTTHADYFYGAIPCTRQMTAEEINGEYEYQTGEVIIETFEERGRSP
                     AQIPAVLVHSHGPFAWGKNAADAVHNAVVLEECAYMGLFSRQLAPQLPAMQNELLDKH
                     YLRKHGANAYYGQ"
     gene            complement(18111..18971)
                     /locus_tag="ACHKIR_003667"
     CDS             complement(18111..18971)
                     /locus_tag="ACHKIR_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFO9991347.1"
                     /translation="MRNHPLGIYEKALAKDLSWPERLVLAKSCGFDFVEMSVDETDER
                     LSRLDWSAAQRASLVTAMIETGVGIPSMCLSAHRRFPFGSRDEAVRERAREIMSKAIR
                     LARDLGIRTIQLAGYDVYYEDHDEGTRQRFAEGLAWTVEQAAASQVMLAVEIMDTAFM
                     NSISKWKKWDEMLASPWFTVYPDVGNLSAWGNDVPAELKLGIDRIAAIHLKDTQPVTE
                     QSPGQFRDVPFGEGCVDFVGIFKTLHELNYRGSFLIEMWTEKAKEPVLEIIQARRWIE
                     ARMQEAGFIC"
     gene            complement(18964..19626)
                     /gene="ulaD"
                     /locus_tag="ACHKIR_003668"
     CDS             complement(18964..19626)
                     /gene="ulaD"
                     /locus_tag="ACHKIR_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFO9991348.1"
                     /translation="MSRPLLQLALDHSSLEDAQRDVTLLKDSVDIVEAGTILCLNEGL
                     GAVKALREQCPNKIIVADWKVADAGETLAQQAFGAGANWMTIICAAPLATVEKGHAMA
                     QRCGGEIQIELFGNWTLDDARDWHRIGVRQAIYHRGRDAQASGQQWGEADLARMKALS
                     DIGLELSITGGITPADLPLFKDIRVKAFIAGRALAGAANPVQVAGDFHAQIDAIWGGA
                     RA"
     gene            complement(19623..21119)
                     /gene="lyxK"
                     /locus_tag="ACHKIR_003669"
     CDS             complement(19623..21119)
                     /gene="lyxK"
                     /locus_tag="ACHKIR_003669"
                     /EC_number="2.7.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-xylulokinase"
                     /protein_id="HFO9991349.1"
                     /translation="MTQYWLGLDCGGSWLKAGLYDREGREAGVQRLPLCALSPQPGWA
                     ERDMAELWQCCMAVIRALLTHSGVSGEQIVGIGISAQGKGLFLLDKNDKPLGNAILSS
                     DRRAMEIVRRWQEDGIPEKLYPLTRQTLWTGHPVSLLRWLKEHEPERYAQIGCVMMTH
                     DYLRWCLTGVKGCEESNISESNLYNMSLGEYDPCLTDWLGIAEINHALPLVVGSAEIC
                     GEITAQTAALTGLKAGTPVVGGLFDVVSTALCAGIEDEFTLNAVMGTWAVTSGITRGL
                     RDGEAHPYVYGRYVNDGQFIVHEASPTSSGNLEWFTTQWGEISFDEINQAVASLPKAG
                     GDLFFLPFLYGSNAGLEMTSGFYGMQAIHTRVHLLQAIYEGVVFSHMTHLNRMRERFT
                     DVHTLRVTGGPAHSDVWMQMLADVSGLRIELPQVEETGCFGAALAARVGTGVYRDFSE
                     AQRDLQHPVRTLLPDMTAHQLYQQKYQRYQHLIAALQGFHARIKEHTL"
     gene            complement(21123..22109)
                     /gene="yiaO"
                     /locus_tag="ACHKIR_003670"
     CDS             complement(21123..22109)
                     /gene="yiaO"
                     /locus_tag="ACHKIR_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter
                     substrate-binding protein YiaO"
                     /protein_id="HFO9991350.1"
                     /translation="MKLRSVTYALFIAGLAAFSTSSLAAQSLRFGYETSQTDSQHIAA
                     KKFNDLLQERTKGELKLKLFPDSTLGNAQAMISGVRGGTIDMEMSGSNNFAGLSPVMN
                     LLDVPFLFRDTAHAHKTLDGKVGDDLKASLEGKGLKVLAYWENGWRDVTNSRAPVKTP
                     ADLKGLKIRTNNSPMNIAAFKVFGANPIPMPFAEVYTGLETRTIDAQEHPINVVWSAK
                     FFEVQKYLSLTHHAYSPLLVVINKAKFDGLSPEFQQALVSSAQEAGNYQRKLVAEDQQ
                     KIIDGMKEAGVEVITDLDRKAFSDALGTQVRDMFVKDVPQGAELLKAVDEVQ"
     gene            complement(22122..23399)
                     /gene="yiaN"
                     /locus_tag="ACHKIR_003671"
     CDS             complement(22122..23399)
                     /gene="yiaN"
                     /locus_tag="ACHKIR_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter large
                     permease YiaN"
                     /protein_id="HFO9991351.1"
                     /translation="MAVLIFLGCLLGGIAIGLPIAWALLLCGAALMFWLDMFDVQIMA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLSKRIVDLPMKLVGHKPGGLGYVGVLAAM
                     IMASLSGSAVADTAAVAALLVPMMRSANYPVNRAAGLIASGGIIAPIIPPSIPFIIFG
                     VSSGLSISKLFMAGIAPGMMMGATLMLTWWWQASRLNLPRQQKATMQEIWHSFVSGIW
                     ALFLPVIIIGGFRSGLFTPTEAGAVAAFYALFVATVIYREMTFATLWHVLIGAAKTTS
                     VVMFLVASAQVSAWLITIAELPMMVSDLLQPLVDSPRLLFIVIMVAILIVGMVMDLTP
                     TVLILTPVLMPLVKEAGIDPIYFGVMFIINCSIGLITPPIGNVLNVISGVAKLKFDDA
                     VRGVFPYVLVLYSLLVVFVFIPDLIILPLKWIN"
     gene            complement(23402..>23765)
                     /locus_tag="ACHKIR_003672"
     CDS             complement(23402..>23765)
                     /locus_tag="ACHKIR_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HFO9991352.1"
                     /translation="DELSRYLFVWLTFIGAIVAFMDNAHVQVTFLVEKLSPAWQRRVA
                     LVTHSLILFICGALAWGATLKTIQDWSDYSPILGLPIGLMYAACLPTSLVIAFFELRH
                     LYQLITRSNSLTSPPQGA"
BASE COUNT         5718 a         6069 c         6405 g         5573 t
ORIGIN      
        1 gactcgctgc ggctgtaacc gtcctcaccg ggaaacaggt gctcaaaata caggcttctg
       61 ccgccgttat cactgagtat cagtgtgtta tcgcgcagct gtaagcggat atccgcaggc
      121 attttccagc cggggccgag gctccccacc ggcgcgggcg tttttgtccg gtaactgctg
      181 taggtgcggg agaggatgaa cggcagcggg ccgggcaggg caaggtcggt ttcgccggga
      241 aggaccttcg caccgagcag cggattgacc ggatggccgg acgtcacccc gccggggcac
      301 accgaacagg ccacgccggt gggggcacca atacgtaccc cggctgaacc ctgaacaatg
      361 ctaccgccat actgcgtcat gtcgccctga cgcgccgccg gttttccgct catctgctca
      421 tccttgtttt atcgggttat ttgtctgctg acaggcgaaa ccctacagat ttataagaaa
      481 gccttaagtg agatatctgt cagataagaa caattaggaa aatatttata tcaccatgat
      541 aaatatgtga tttattggga agtaacaaac catcaacatt ttctgccctt tttcctatca
      601 ccaggccata ctgatatttt tcctcgcaca aaggactcgg catgaaactc gtcggtagct
      661 acaccagccc gtttgtacgc aaactttcta ttctgttgtt agaaaagggc ataactttcg
      721 aatttattaa tgaactgccc tataacgcgg ataacggcgt ggcgcaattt aacccgttag
      781 ggaaagtgcc ggtgctggtg accgaagagg gcgaatgctg gtttgattcg ccgatcatcg
      841 ctgaatatat tgaattaatg aatgtcgctc cggcgatgtt gccgcgcgat ccgctggagt
      901 cgttgcgggt gcgcaaaatt gaggcactgg cggatggcat tatggatgcc gggctggtat
      961 cggtgcgtga acaggcgcgt ccagcggcgc agcagtctga agatgaattg ttacgccagc
     1021 gcgagaaaat caaccgcagt ctggatgtgc tggaaggata tctggtcgat ggcacgctca
     1081 aaaccgatac ggtcaatctg gcgactatcg ccattgcctg tgccgtcgga tatctcaatt
     1141 tccgccgcgt tgcgccaggc tggtgtgtcg accgcccaca cttagtcaaa ctggtcgaaa
     1201 acctgtttag ccgcgaaagt tttgctcgca ccgaaccgcc aaaggcttga tgcgcgatat
     1261 gtcctcctga cccatctcac gttacaatcc gtggttatgt taaacgccct tctccgtgtg
     1321 agagggcctt gatcagccag gtttcctatg acaaccgaaa cgcgttccct ctatagtcaa
     1381 cttccggcta ttgatcgctt attgcgcgat agctccttcc tttctttgcg tgatacttat
     1441 ggtcacaccc gcgtggtgga attgctgcgt cagatgcttg acgaagcgcg agaagtgatt
     1501 cgtgatagcc agacgctgcc cgcatggtgt gaaaactggg cgcaagaagt cgatgcccgg
     1561 ttgacgaaag aggcgcagag cgcgctgcgt ccggtgatca acctgacggg aaccgtgctg
     1621 cataccaatc ttgggcgagc tttacaggca gaagccgcgg tggaagccgt tgcgcaggct
     1681 atgcgttcgc cagtgaccct cgagtacgat ctggacgacg ccggacgcgg acatcgcgat
     1741 cgggcgctgg cgcagctgct gtgccgcatt acgggagcgg aagatgcctg tatcgttaat
     1801 aacaatgcgg cggcggtgtt attgatgttg gcggccactg ccagcgggaa agaggtggtg
     1861 gtatctcgcg gcgaactggt ggagattggc ggcgcgtttc gtattcctga tgtcatgcgt
     1921 caggcaggct gcaccctaca cgaagtgggg accaccaacc gcacgcacgc taatgattat
     1981 cgtcaggcgg tgaatgaaaa taccgcgctg ttgatgaaag tacataccag taactacagc
     2041 attcagggat tcaccaaagc gatagatgaa gcggaactgg tggcgctcgg caaagagctg
     2101 gatgttcccg tagtgactga tttaggcagt ggctcgctgg ttgatcttag ccagtacggt
     2161 ttgccgaaag agccaatgcc gcaggagttg attgcggcgg gcgtcagtct ggtgagtttc
     2221 tccggtgaca agttgttagg cgggccgcag gcaggaatta ttgttggtaa aaaagagatg
     2281 atcgcccgcc tgcaaagcca cccgctgaag cgtgcattac gcgcggataa aatgaccctc
     2341 gcggcgctgg aagccacgtt gcgtctttat ttacaccctg aagctctgag tgaaaaatta
     2401 ccgaccctgc gcctgcttac ccgcagcgca gaggtcattc aaatccaggc acaacgttta
     2461 caggctcccc ttgccgcaca ttacggcgcg gagtttgcag tccaggttat gccatgtctt
     2521 tcgcagattg gcagtggttc actgccggtt gatcgcctgc cgagcgcggc attaacgttt
     2581 acaccccatg atggacgcgg tagccacctt gagtcattag ccgcccgctg gcgtgaattg
     2641 ccagtgccgg tgattggtcg tatttatgac ggacgattgt ggctggattt acgctgcctt
     2701 gaagatgagc aacggttttt ggagatgttg ttgaaatgat tattgcgact gccggacacg
     2761 ttgaccacgg caaaacaacc ttattgcagg cgattactgg cgtaaatgcc gatcgtctgc
     2821 cggaagaaaa aaagcgcggc atgactatcg atctcggcta tgcctactgg ccgcagccgg
     2881 atggccgcgt gcctggtttt atcgacgttc ccggtcatga aaagtttctt tccaacatgc
     2941 tggcgggcgt cggtggtatc gatcacgcgc tgttggtggt ggcgtgcgat gacggtgtga
     3001 tggcacagac ccgtgagcat ctggcgattt tgcagctgac tggtaacccg atgctgacag
     3061 tggcgctgac caaagccgat cgcgtggacg aagcgcgtgt tgatgaggtt gaacgccagg
     3121 taaaggaggt gctgcgggaa tacggttttg ctgaggcaaa actgtttatc accgcagcaa
     3181 ccgaaggtcg gggaattgat gccctgcgcg agcatctgct tcagttacca gaacgcgaac
     3241 acgccagcca acatagtttc cgcctcgcga tcgaccgtgc atttaccgta aaaggtgccg
     3301 ggctggtcgt caccggtacg gcgttaagcg gggaagtgaa ggtaggcgat tcactctggc
     3361 tgactggtgt aaataaaccg atgcgcgtgc gtgcgctgca tgcgcaaaat cagccaacag
     3421 aaactgccca tgccgggcag cgtatcgcgc ttaacatcgc gggtgatgcg gaaaaagagc
     3481 agattaaccg tggcgactgg ctgcttgccg atgcgccgcc ggagccgttc acacgggtga
     3541 ttgtcgagct tcaaacccat acaccgctga cccagtggca gccgctgcat attcaccacg
     3601 ccgccagcca cgtcacggga cgcgtttcgc tgctggaaga taaccttgcc gagctggtgt
     3661 tcgacacgcc gctgtggctg gcggataacg atcgcttagt gttgcgcgat atctcggccc
     3721 gcaacacgct ggcgggggcg cgtgtggtga tgcttaaccc gccgcgtcgc ggtaagcgta
     3781 agccggaata tctgcaatgg ctggcgtctc ttgcacgggc gcagagcgat gccgatgcgt
     3841 tatctgttca tctggaacgc ggagcggtta accttgcgga tttcgcctgg gcacggcagc
     3901 tcaacggcga agggatgcgc gaactgctgc aacagcctgg ctatattcag gctggttata
     3961 gcttgttgaa tgcgccggtt gccgctcgct ggcagcggaa aattctcgac acattagcga
     4021 cttatcatga gcaacatcgc gatgaacctg gtcctgggcg agaacgtctg cgacgtatgg
     4081 cgttgccaat ggaagatgaa gcgctggtac tgttgctgat tgaaaagatg cgcgaaagcg
     4141 gcgacatcct cagtcatcac ggctggctgc atctgccgga tcacaaagcg ggcttcagcg
     4201 aagagcagca ggccatctgg caaaaagcag agccactgtt tggtgacgaa ccgtggtggg
     4261 tgcgagacct ggcaaaagaa acgggaaccg acgagcaggc aatgcgcctg acgctacgcc
     4321 aggcggcgca acaggggata attactgcga tcgtcaaaga tcgttattac cgtaacgatc
     4381 ggattgtcga atttgccaat atgatccgcg atctcgatca ggaatgtggt tcaacctgcg
     4441 cggcggattt ccgcgatcgc ttaggcgtag gccgaaagct ggcaattcaa atcctggaat
     4501 attttgaccg cattggcttt acgcgtcgtc gtggaaatga tcatttattg cgcgacgcat
     4561 tattatttcc ggaaaaataa ggaaatgatt aattcataat aaatatgaat taatacaaaa
     4621 tttcttatta atgttaagaa ggcacgttat ttgatgaatg taacgtgctt tttattaaaa
     4681 atattttaat aaacggtaat gttgatcata ttttcaaatg aaaatcctca gtaagctgcc
     4741 cgcccttttt tacactttca ggagtgtgtt atggcagctt caaccttttt tattccttcc
     4801 gtgaatgtta ttggtgctga ttcattgaat gatgcaatga atatgatggc agattatgga
     4861 tttacccgta ccttaattgt caccgacaac atgttaacga aattgggtat ggcgggtgat
     4921 gtgcaaaaag cactggaaga acgcaatatt tttagcgtta tttatgatgg cacccaacct
     4981 aaccccacca cggaaaacgt cgccgaaggt ttaaaattac ttaaagaaaa taattgcgat
     5041 agcgtgatct ccttaggcgg tggttctccg cacgactgcg caaaaggtat tgcgctggtg
     5101 gcagccaatg gcggcgatat tcgcgattac gaaggcgttg accgctctgc aaaaccgcag
     5161 ctgccgatga tcgccatcaa taccacggcg ggtacggcct ctgaaatgac ccgtttctgc
     5221 atcatcactg acgaagcgcg tcatatcaaa atggcgattg ttgataaaca tgtaactccg
     5281 ttgctttctg tcaatgactc atctctgatg gtcggtatgc cgaagtcact gaccgccgca
     5341 acgggtatgg atgccttaac gcacgctatc gaagcgtatg tttctattgc cgccacgccg
     5401 atcactgacg cttgcgcact gaaagccgtg accatgattg ccgaaaacct gccgttagcc
     5461 gttgaagatg gcagcaatgc taaagcgcgt gaagcaatgg cttatgccca gttcctcgct
     5521 ggcatggcat tcaacaacgc atctcttggt tatgttcatg cgatggcgca ccaactgggc
     5581 ggtttctaca acctgccaca tggtgtatgt aatgccgttc tgctgccaca cgttcaggtg
     5641 ttcaacagca aagtcgctgc tgcgcgtctg cgtgactgtg ctgctgccat gggcgttaac
     5701 gtgacaggca aaaatgacgc ggaaggcgca gaagcgtgca tcaatgccat ccgtgaactg
     5761 gcgaagaaag tgaatatccc ggcaggcctg cgtgacctga acgtgaaaga agaagatatt
     5821 ccagtgctgg caaccaatgc cctgaaagat gcgtgtgggt ttactaaccc gattcaggca
     5881 actcacgaag aaattatggc gatttatcgc gcagcgatgt aatcatcatt cccacaatgg
     5941 ctgtctaatt gtcagccatt ttttaaacta tctcaaaatt tgcgattttt gagatagttt
     6001 ctctttcctg ctatacacta tctcaatatt gcgctttttt gagataagca catggctatc
     6061 aaatcacctc ccggacttat tcctttatca cacttatcgg gtgaggaact cctggcccac
     6121 ctgcgcttta accgggttac cgatgaaaaa ggacgttatc tgccttttga tgagctgcag
     6181 taccgcatca aaaaaggaga aaacgtcgat gtggcgtgga cgcttacccg tctggcgcgc
     6241 aacgcggcga tccaacgaat taattattgc aatgaagcgg gtgaacaggc cggattcaac
     6301 attacgccgg tgattgcaga agcgtgcgag cttgttgata aacatgccac tgcactggcg
     6361 ctgaaagatc agactgaacg gttacgcggg gcgggcgccg agttaagtca gttacgcctg
     6421 gaagagccga tcaccagttc ccaactggaa ggtgcgaata ctacaacgct ggtggcgaga
     6481 aaaatgctgg aaacagggcg gtcgccacgc actgaagacg agcatatgat tgcgggaaat
     6541 gcccggctga tggcggaaat tccgcactta ctcgcagaac cgctgacacc tgcgcttatt
     6601 cgccagttac atgccattgg tatgggtgga atcaatgacg cgaaatatcg tccgggagaa
     6661 tttcgtgaaa cagatgatgt tgtgatcgcc gactatgatg gcaatatagt gcatcaaccg
     6721 cccgctgcgg cattgttacc ggaacggctg gaaaaagttt gccagtggtt aaacagccat
     6781 gaagggtata ttcatccgtt ggtccgtgcc tgcattctgc attttatgct ggcccatgaa
     6841 catccgtttc gcgatggcaa cgggcgcacc agtcgggcgc tgttttactg gtatatgctg
     6901 aaatcgggct acgatgtctt taaatatatt tccatcagcc gtttgcttca tgctgcaccg
     6961 gtcaaatatg ccgccagcta ccagtacact gaatcagatg gtatggatct gacttatttc
     7021 cttgaatatc aggcgggtgt cattaaacgt gctctgcaaa actggcagca gcatatcgat
     7081 gaaattacac aacgaagcgc aaaactcgat agtgtgctgt ttagctctgg tgtgcttaaa
     7141 cgcctgaatc cgcgccaggt aacgctcctg aatgtgatgc tcgccaatcc ggggaaagag
     7201 tacacagttg ctgaaattag cgcgagcttg ggcgtctctg ataatacggc tcgagctgat
     7261 ttacggacaa ttgttaaaga aggattcgcg caagagaaaa gaattaataa ccagcaagcc
     7321 gtgtacgtcg cacactaccc gctgtagctt ttgcccgtaa attcgtgata gccgtcgtaa
     7381 agctgttacc gactggcgaa gatttcgcca gtcacgtcta cccttgttat acctcacacc
     7441 gcaaggagac gatcatgacc aataatcccc cttcagcaca gattaagccc ggcgagtatg
     7501 gtttccccct caaattaaaa gcccgctacg acaactttat cggtggcgaa tgggtagcgc
     7561 ctgccgatgg cgagtattac cagaacctga cgccggtgac cgggcagctg ctgtgcgaag
     7621 tggcgtcttc gggcaaacga gacatcgatc tggcgctgga tgctgcgcac aaagtgaaag
     7681 ataaatgggc gcacacctcg gtgcaggatc gtgcggcgat tctgtttaag attgccgatc
     7741 gaatggaaca aaaccttgaa ctgttagcga ccgctgaaac ctgggataac ggcaaaccca
     7801 ttcgcgaaac cagtgctgct gatgtgccgc tggcgattga tcattttcgc tattttgcct
     7861 cgtgtattcg ggcgcaggaa ggtgggatca gtgaagttga tagcgaaacc gtggcctatc
     7921 atttccatga gccgttaggc gtggtggggc agattattcc gtggaacttc ccgctgctga
     7981 tggcgagctg gaaaatggct cccgcgctgg cggcgggcaa ctgtgtggtg ctgaaacccg
     8041 cacgtcttac cccgctttct gtactgctgc taatggaaat cgtcggtgat ttactgccgc
     8101 cgggcgtggt gaacgtggtc aacggcgcag gtggggaaat tggcgaatat ctggcgacct
     8161 cgaaacgcat cgccaaagtg gcgtttaccg gctcaacgga agtgggccaa caaattatgc
     8221 aatacgccac gcaaaacatt attccggtga cgctggagct gggtggcaaa tcgccaaata
     8281 tcttctttgc tgacgtaatg gatgaagaag atgccttttt cgataaagcg ctggaaggct
     8341 ttgcactgtt tgcctttaac cagggcgaag tttgcacctg tccaagccgt gctttagtgc
     8401 aggagtctat ctacgaacgc tttatggaac gcgccatccg ccgtgtcgaa agcattcgca
     8461 gcggtaaccc gctcgacagc gtgacgcaaa tgggggcgca ggtttctcac gggcaactgg
     8521 aaaccatcct caactacatt gatatcggta aaaaagaggg cgcagacgtg ctcactggcg
     8581 ggcggcgcaa gctgctggaa ggtgaactga aagacggcta ctacctcgaa ccgacgattt
     8641 tgtttggtca gaacaatatg cgcgtgttcc aggaggagat ttttggcccg gtgctggcgg
     8701 tgaccacctt caaaacgatg gaagaagcgc tggagctggc gaacgatacg caatatggcc
     8761 tgggcgcggg cgtctggagc cgcaacggta atcttgccta taagatgggg cgcggcatac
     8821 aggcagggcg cgtgtggacc aattgttatc acgcttaccc ggcacatgcg gcgtttggtg
     8881 gctacaaaca gtcgggcatc ggacgcgaaa cccacaagat gatgcttgag cattaccagc
     8941 aaaccaagtg cctgctggtg agctactcgg ataaaccgtt ggggctgttc tgatataaga
     9001 agctggtcgc attgggtatt cattgcctga tgcgacgctt acgcgtctta tcatgcctac
     9061 ggggacctga ccgtaggccg gataaggcgt acacgccgca tccggcataa acaaagcgca
     9121 cgttgttaca atttacgttt taaacctacg tgcgcaataa cccagactac gtcaacagaa
     9181 gtgcgtttct ggggcgtgtt ttgccaggag tttaatctcg cgtttcgatg gtgagcgggc
     9241 gaaccaggcc ataaatcacc attggcgttc cgaggccaat cagcaataaa cggtagataa
     9301 ttgaaggttt aatacgctta atctgtactg tggatgtttt actggagtta ttcattagtt
     9361 tggccaatag ttccattatt tcaatatgat aagcctcgca ctgttgaaaa gccaggctgc
     9421 acacttcaga aagtatataa aaaacaatat gtcgttatat aaataaggaa attgcaccgc
     9481 attatggtat ttcataatat gcgttaattg ttcttttcta tattctctga gggaaatatg
     9541 ttcctggatt attttgcatt gggagtgctt atttttgtat ttctggtgat attctatggg
     9601 attattattt tacatgatgt tccctacctg attgccaaaa aacgtaatca tcctcatgcc
     9661 gacgccattc atgttgctgg ttgggtgagt ctttttacgt tacatgttat ctggccattt
     9721 ttgtggattt gggccacgct ttaccgcccg gagcgtggat ggggaatgca aaaccatgac
     9781 tcctccgttg tgcaactaca acagcgtatt gccgggctgg agaaacagct cgctgacatc
     9841 aaatcctctt ctgccgagta atacttatgg atttactgat tattttaacc tatgtggctt
     9901 tcgcatgggc aatgtttaag atcttcaaaa ttcctgtaaa taaatggacc attcccacag
     9961 cggccctggg aggcatattt attgtcagcg gtctaattct gttaatgaac tataaccatc
    10021 cgtatacctt taaagcgcaa aaagcggtta tttctattcc tgttgtccca caggtgacag
    10081 gcgtggtgat cgaagtgacg gacaagaaaa atacgctgat taaaaaaggt gaggtgctat
    10141 ttcgactgga cccgacgcgt tatcaggctc gggtggatcg gctgatggcg gatatcgtta
    10201 ccgcagaaca taaacagcgg gcgttgggcg cagagttaga tgagatggcg gcgaatactc
    10261 agcaggcaaa ggccacgcgg gataagttcg ctaaagagta tcagcgttac gcacgtggca
    10321 gccaggcgaa agtaaacccg ttttcagaac gcgatatcga tgtggcgcgg caaaattatc
    10381 tggcgcagga agcctccgta aaatcagcgg cggcggaaca aaaacagatc cagagccagc
    10441 tggatagcct ggtgttgggt gaacattctc aaatcgccag cctgaaagcg cagctcgcgg
    10501 aagcaaaata taaccttgag cagacgatag tgcgtgcgcc gagcgatggt tatgtgaccc
    10561 aggtgctgat tcgtccgggt acctatgccg cgtcgctgcc gctacgtccg gtgatggtgt
    10621 ttatacccga tcagaaacga caaatcgtgg cgcagttccg tcagaactcc ttgctgcgcc
    10681 tggctcccgg cgatgatgcg gaagtggtat ttaacgctct gccaggcaaa gtgttcagcg
    10741 gtaagctggc agccattagt ccagctgttc ccggtggagc ttatcagtcg accggcacct
    10801 tacagacgtt aaacacagcg ccgggttcag atggcgttat cgcgaccatt gaactggatg
    10861 agcatactga tttgagcgca ttaccagacg gtatttacgc ccaggtggcg gtctattctg
    10921 atcatttcag ccatgtctcg gtgatgcgca aagtactgtt acgcatgacc agctgggtgc
    10981 attaccttta tctcgatcat taatcttcgc tttccgccgc ttgttcaggc cgcagttgct
    11041 gcgactgagc aagctggtgg cgaatttcac gcagcatttc atccagcaca tactggaccc
    11101 gccagggctg gtattcccgt ttacggaaga tagccaccag atccagccac cattcatacg
    11161 ggtgctgaaa gcagggcacc agcgtgccgt cttgcaggtc gctctgcaca cttttgtctg
    11221 gcgcaaacac aatccccagg tgatttcgtg ccagctccag cgcagattgc gtgttgtcgc
    11281 aaacgtaatt tcccttcacg cgataatccc gcacctcttt actcccggcg acgttgaagc
    11341 gccagatatt cgcgtcgtcg atcatcatcg agtcgatcaa aatacaggag tgatgttcga
    11401 gttcgtcagg acggctaatg ggatgtttct caagatagcg ctggctggca taagcggtta
    11461 cggcatattg ggtaataaaa ctggcaacca gcgattcatc ttttggttgg gcgtagctga
    11521 ttaaaacatc acaatcatcg ggaaattcga cgccttcata aaaggcatta cgctcaagat
    11581 tgcaggtttt tagcgataag gtgatatcac cgatatcttt aattttatcg attacatgtt
    11641 tggataaata ggtaattatg ccggttggtg catagatagt gacccgacca cgtttctcat
    11701 gcttataatc tgcaataaaa ttattaagct gctcgtttct gtccagcatg tcgttgatat
    11761 acggtaacag cacggtgcca aaaggtgtga gcatcagctg tcgggtggtt cggtcaaaga
    11821 cttttaaacc cacttttgat tcaaaatcag caagatattt gctgacgttg gcctgtgcga
    11881 tgccaagtac agtcgcggca tggctgatat tttcactggc agcgattacc gagataattt
    11941 ttaactcccg gtacttaagt tgtaattttg tcatcgcaac agcccaatta tatataattt
    12001 tatatattac tatataaata tgaatcttgc accggagaaa ttgtaagcat tactatgccg
    12061 actctttttt atcttatttt aaaatggaat accggacatg ttaattaacc gcaatattgt
    12121 ggcgttattt gcgttgcctt ttatggcaag cgcaactgct tctgaattat ccattggtgc
    12181 tggtgcggct tataatgaat cgccttatcg cggttataat gaaaatacaa aggcaattcc
    12241 gctgattagt tatgaaggtg atacttttta tgttcgtcag accacgttag gttttattct
    12301 gtcgcaaagt gaaaaaaatg aacttagcct gaccgcatcc tggatgccgc tggaatttga
    12361 ccctaccgat aatgacgatt atgccatgca acagcttgat aagcgtgata gtacggctat
    12421 ggcgggggtt gcctggtatc accacgagcg ttggggaacc gtgaaagcct ctgcggctgc
    12481 ggacgttctg gataacagca acggctgggt gggggagcta tcggtattcc acaaaatgca
    12541 gataggtcgc ctgtcgctga cacctgcgct gggcgttctc tattatgacg agaatttcag
    12601 tgactattac tatggcattt cagagagtga gtcccgtcgt agcggtctgg caagttattc
    12661 cgcgcaggat gcctgggtgc cttatgtcag cctgacggca aaatacccga taggagagca
    12721 cgtcgtattg atggcgagcg ccggatacag cgagctgccg gaagagatta ccgacagccc
    12781 gatgattgat cgtaatgaga gttttacctt tgtcaccggc gtgagctggc gtttttaatt
    12841 taccggtgga tgtcggtgcg gctggcctac gctctcgcac cgactcgtgg gcctgataag
    12901 acacgttaag cgtcgcatca ggcaatgtgc tccctacgtt ggatgcggcg cgagcgcctt
    12961 atccaacctc cggatggcaa tgcttttcct cattcaccca gatccgcatt tcgccttcac
    13021 cccggttggc ccagctaaac cacgggataa aggtcagggt ttgcggctgg cgtttggctg
    13081 gcgcgctgtc gtaatgccac agcggttgct gctctggatt gctctgttca taccgataac
    13141 ccggtgcctg gattaagatc ttatggcgga acaatccctt accttcaaat gtcgtaaatg
    13201 gcgcatcggc gggcagccac agattatgca gtgactcgcc gttgtcggcc tgttccaggc
    13261 aatacaccag cgggccgcgc tgaatcgcca cttttccggc gacgtgacgc accagagggt
    13321 taccgtaaac gcgacgtacc ggcattggca aagtcagatt cagcgtgtcg ccctcctgcc
    13381 attcgcgggt aatgtgcaaa taccctttac gaatatcctg ctcgacctct tccccattca
    13441 atatgatctg cggctgtgtg caccagtccg gcagacgtaa agccagcgta tgacgcaccg
    13501 gctggggcga ttcaaccgca attgtcacct gttcctgcca cggatagttt ccgctaaccc
    13561 gcagacgcag cgtgccattt tctaccggca cttccatgct gtttcctgcg tagatgttga
    13621 tatacaacgc atcttcacgc ggcgtgtaga gataatgacc aatcgaggtt agcacgcggg
    13681 cgatatttgg cggacaacaa gcgcaaccaa accaacgctg gcggatcggc ttaacgtgat
    13741 cgtagatatg gttgaatttt aacgtttttg gatgcacttc cagcggatta acgtagaaga
    13801 aatgtttgcc atccagcgcc atgccgccga gcacagtgtt gtacagtgcg cgctccatca
    13861 catcggcata ttgactgtcg ccttccattt ccagcattcg ccgggcgaac atcatcaggc
    13921 cgatggaagc acaactttcg gcgtaaaccg tgtcattcgg cagatcgtaa tcgctgctga
    13981 acgcttcgcc gctgctttgt gagccaatgc cgccggtaat atataactga cgctgggcca
    14041 tattgttcca cagccgcagg caatcctgac gcttgctttc atcgtgactt aaacgcgcga
    14101 gatgtgcgac gccagtcatc agatatacaa agcgtactgt atgtccgatg gcggtttgtt
    14161 gctgtgcgat gggcaaatgc gcctggctgt aggctttatc tttcaccatc cacgccgggc
    14221 cgtaggtgtg ccagtgcgag gtctgcccgc gcttttcata ttcctggtcg taatagtgcg
    14281 gttgcgcacc acgctgttcg acaaaatagt tcgtcagcgc caggtagcgc ggctcttcgg
    14341 tcacttcata caggcgcatt agtgccagtt caatttccgg gtgaccagga taaccgtgta
    14401 acttactttc acctggacca aatacgctgt cgatatgatc ggccagacgg caaaccactt
    14461 ccagcaagcg ccgttttccc gtggcctgga agaaggcgac tccggcttca atcagatgac
    14521 cggcgcagta aagttcatga cactccgcca gattgctcca gcgttcttcg ggcgctttta
    14581 ccgtaaagta agcattgaga tagccatctt cgcactgggc ggaggcgatc agttcaatca
    14641 cctcgtcagc ggttttttcc agttcggcat ccggcttctg gcacagcgac caggctaccg
    14701 cttccagcca tttggcgacg tcgctgtcct gaaacaccat cccgtaaaat tcaccgtctt
    14761 gcagtcctgc ggcaatgcga aagttttcaa tcgcatggct gggttccgct tctgggatac
    14821 gatcgttcaa ggcgtcccac tgataaggaa tcaccacatc gcggaccagt tgttggtact
    14881 gaccgaggaa cggatcgctg accgtcagtt tatgcagatc gacttccgaa atgttcatct
    14941 tgtgctcctt aaggctgtac atgacgctga cgcaggtcgc tagcaatgcg tgacatcata
    15001 gggttattga gtttgcaaaa gcggatcgcc cctgccagca gcaggtggaa tagcgcaggc
    15061 agtaatgttt ccatcgccgt gatcccgcca agcgaactgg tggtctggtt ttccacgcct
    15121 ggctggtagg cgacaaaaat aaacagcagg ctgatgatcc cggcgctgga ggcccatgcc
    15181 agcttgatga aaaacagatt gaaggcgaag ttcatgccgg aagaacgtac gcgggttttc
    15241 cactcgccgt agtcatcggc aaaggccatc aatgagaagt gcagtggcaa tgtgaagcca
    15301 agaatcacgc cattaccgag gatcaccgcc agccacagcg tttgataagc cgggccagaa
    15361 ggcaggaacc acatcaacac cgccagcgca gcgaggagca ggttggtgta gtagtaaatt
    15421 ttgacggtat cgaaacgccg cgttaacggg ctgacaatca ccgagccgat aatggaggcg
    15481 aaggtgacca tggtgaagaa cagcgacgtg tagcccgtac tgccttgtaa gacgtaggta
    15541 atgaagtaca tatacccacc gccgcgaata ttaaagacgt taatcagcag gaaagacatg
    15601 accagcatca gcagcagttg gtcgttgttc cgcagcccgg caagatgttc gcgcagggta
    15661 aatttcccca ttgtggagag tggcacccgt tcacgaaccc agaagaaaca gcacaggaac
    15721 atcaccacgg caatggcgca cagcacgccg acgcccagtt gatagccgcg cgcggcatta
    15781 ccctgaccga agagatccac catccacggc aggccaaccg aaaccagaaa tcccgctacg
    15841 ccgcagagaa cgaatcgcca ggactggcag gagatcactt cattgtggcg ggtggtcatg
    15901 gtgttgatca gcgcgcagta cggtacgttg atggcggtat aaccgactga aaggagcagg
    15961 taagtaccga acgcccaggc gatttttact cccatgctgg catctggcac ggtgaacgtc
    16021 aggatgccga taatgccgat gggaatggca atccataact gccacggacg aaagcgcccc
    16081 cagcgacttt gcgtccggtc ggcaatgacg cccatcaccg ggtcggagat ggcatcaaac
    16141 acgcgcagtg caataaacag cgtgccgacc agcgccggag ttaagccaaa aacgtcggtg
    16201 tagaaaaagg tgagaaagtt catgatcagg caagttatta cggtgccgcc tgcgtcaccc
    16261 aggccgtagc ctattttttc ccgtacagat aacgcatcgt caattttacc tttagcgata
    16321 tcagcggcag taatcggtgt agaggtcata gatattcctc gtgaataggg agcaattttt
    16381 agttttattg caatgacctg attaattctg ccgtgaacgg gaatatcaac aaggcgagaa
    16441 aagagtaaaa aaaggtgacg attccgacct gaatttaaaa gtgtgaaatg gatcaggtga
    16501 aattaatgtt tttgattaat aatcaaaagg taatgtgttt aatcattaat gaataagcgg
    16561 gaaattacat atcaatgctc gaattatcca tgacgttacc gattaaagtg caaaatggtg
    16621 gattatttat ttcccgtggc gtagggaggc acccggcaag gcggctggag tcatgggaaa
    16681 ttatctttgt cgaaaaaggt cgattaacga ttcaggaaga agagcggata tttctggtcg
    16741 atgcgggaga gagtttatta ctctggccga accgacagca tgttggggta gaggattttc
    16801 ccgccgatct caaattttac tggctgcatt ttgaggttaa agcgccggat agcgacccgc
    16861 gctggctcac tcatcttagc gtgccgcaac aaacgaaggt tgccgatccg caggcgctgg
    16921 tggccctgtt tcgccagttc ttaagcgaac aggaaaagca tcaacgcact ccggcgctgg
    16981 agtttattgt gctgctgatt ttgcagcagc tcacccgtga cgcgcgtctt gatgaacagg
    17041 ccgatgccgc aggtaccacg cttgcctgga aagcgcagaa actgattcgc acgcaatatc
    17101 atcgcccgct ttctacggcg cttttagccc gcgagctgta ctgcaatgcc gattatcttg
    17161 ggcgcgtcta ccggcgggtg tttcatctga cgttaacaga agcaattcat cgccagcgag
    17221 tgcgcgaagc cgaaaagctg ttaattagcg aagcgcgatc gctcactgaa gtggctgcgt
    17281 tgtgcgggtt taatgacgtt ggctatttcc gacagatttt tcgcaaacac accggtttaa
    17341 cgccagctgc gtggaagcga cgttacagca aggaacatat caattcgtag tgccggggca
    17401 atgaagcccc ggcgtgaggg attactgccc gtaataggca ttggccccat gcttacgcag
    17461 gtagtgctta tctaacagtt cgttttgcat cgcagggagc tgcggcgcaa gctggcgcga
    17521 gaacaggccc atataggcgc actcttccag cactacggca ttatgcaccg catcggcggc
    17581 attttttccc caggcgaacg ggccgtgaga atgcaccagc accgccggga tttgtgccgg
    17641 actcctgccg cgttcttcga aagtttcaat gatcacttcg ccggtctgat attcatattc
    17701 gccgttaatc tcctctgcgg tcatctgtcg cgtgcagggg atggcaccat agaaataatc
    17761 ggcgtgcgtg gtgccccagg cggggagatc cagcccggcc tgcgaccaga tggtggcgtg
    17821 gcgcgagtgg gtatgcacaa tgccaccgat ttcggcatag cgacggtaaa gcgccagatg
    17881 cgttggcgtg tcggacgagg gctttttgct gccttccacc accttaccgc tggctatctc
    17941 aaccaccacc atatcgtcgg cggtcatcac gtcgtactcg acgccggaag gtttgattac
    18001 catccattgc cgcgcttcgt ctaccgcgct gacattgccc caggtgaacg tcaccagatg
    18061 gtgagcggga agtgccagat ttgccgccag cacgtcggct ttcagttgct ctaacatata
    18121 aaacctgcct cctgcatacg cgcttcaatc cagcgccgcg cctgaataat ctccagcacc
    18181 ggctctttgg ctttttcggt ccacatctca atcaggaaag aaccgcgata gttcagttca
    18241 tgcagcgttt tgaagatgcc aacgaaatcg acgcagcctt cgccaaacgg cacatcacgg
    18301 aactgtccgg ggctttgctc ggtgactggt tgagtatctt tcaggtggat cgcggcgata
    18361 cggtcaatgc ccagtttcag ttcggcggga acatcattgc cccaggcgct gaggttgccg
    18421 acgtccgggt agacggtgaa ccacggcgag gcgagcatct cgtcccactt tttccatttg
    18481 ctgatggagt tcataaacgc agtatccata atctccaccg ccagcattac ttgtgatgcc
    18541 gccgcctgtt cgactgtcca cgccagccct tcagcaaaac gttgccgggt gccttcgtcg
    18601 tggtcttcgt aatagacgtc gtagcccgcc agctggatgg tgcgaatgcc gagatcgcgc
    18661 gccaggcgaa tcgctttact catgatttcc cgcgcccgtt cgcgcactgc ctcgtcacgg
    18721 ctaccaaaag gaaagcgacg atgtgcggac aggcacatcg acgggatccc aacacccgtt
    18781 tcgatcatcg cagtgaccag cgaagcacgt tgtgcggcgc tccagtcaag acgtgagagc
    18841 cgttcgtcgg tttcatccac cgacatttcg acaaaatcaa agccacagct ttttgccagc
    18901 accagccgct ccggccagga gagatctttc gccagcgctt tttcataaat ccctaacgga
    18961 tgattacgca cgcgcacctc cccagatggc atcaatttgt gcatggaaat cgccagccac
    19021 ctgcaccgga tttgctgcgc ctgccagtgc tcgcccggca ataaacgctt tcacgcgaat
    19081 atctttaaac agcggcaggt cagcaggggt aatcccgccg gtaatggaaa gctcaaggcc
    19141 gatatcggaa agcgccttca tgcgtgccag atcggcctcg ccccactgtt gcccgctggc
    19201 ctgcgcgtcg cgaccgcgat gataaatggc ctgccgcacg ccaatacgat gccagtcgcg
    19261 ggcgtcgtcc agcgtccagt taccgaacaa ctcgatctga atttcaccac cgcagcgttg
    19321 cgccatcgcg tggccttttt ccaccgtcgc gagcggtgcg gcgcagatga tggtcatcca
    19381 gttagcgcct gcgccaaacg cctgttgcgc gagcgtttca ccggcatcgg cgaccttcca
    19441 gtcagcgacg atgattttgt tcgggcactg ttcgcgcaag gctttcactg cgccaagccc
    19501 ttcgtttaaa cagagaatgg tgcccgcttc gacgatatcg acgctgtctt ttaacagcgt
    19561 cacgtcgcgc tgtgcgtctt caagtgatga gtggtcgagg gccagttgca gaagtggtcg
    19621 gctcataatg tgtgctcctt gatgcgggcg tgaaagccct gaagtgcggc aatgagatgc
    19681 tgataacgtt ggtatttttg ttggtaaagc tgatgggcgg tcatatccgg cagcagggtg
    19741 cgtaccgggt gctgtaaatc acgttgggct tcgctgaaat cgcgataaac tccggtgccg
    19801 acgcgggcgg caagtgccgc accaaagcag ccggtttctt ccacctgcgg cagctcgata
    19861 cgcagaccgc tgacgtccgc cagcatttgc atccagacat cggagtgcgc cggaccgcca
    19921 gtgacgcgca gggtgtgcac atcagtaaaa cgttcgcgca tccggttgag atgggtcata
    19981 tggctgaaca ccacgccttc ataaatggcc tgcaacaggt gtacgcgggt gtgaatggcc
    20041 tgcatcccgt agaaaccgct ggtcatctcg agtccagcgt tgctgccgta caggaacggc
    20101 aggaaaaaga gatcgccccc ggccttcggc aagctggcaa cggcctggtt gatctcatca
    20161 aacgagattt ctccccactg tgtggtaaac cattcgagat tgccggaaga ggtggggctt
    20221 gcttcatgaa cgataaattg accatcgtta acgtagcgac catagacata cggatgcgct
    20281 tcaccgtcac gtaaaccacg ggttatgccg ctggtcaccg cccaggtgcc catcaccgca
    20341 ttgagggtaa attcgtcttc gatcccggcg cagagtgcgg tggaaaccac atcaaacagg
    20401 ccgccgacaa cgggcgtacc cgctttcaga ccggtcagtg cggctgtctg agcggtgatc
    20461 tccccgcaga tttcggcaga tccgacaaca agtggcaggg cgtggttgat ttcagcgatc
    20521 cccagccagt cggtgaggca cgggtcatat tccccaagac tcatgttgta gagattggac
    20581 tcagaaatat tgctctcttc gcaacctttg acgccggtta aacaccagcg caggtagtcg
    20641 tgcgtcatca tcacgcagcc aatttgcgcg tagcgttccg gttcgtgctc tttcagccag
    20701 cgtaacagcg acaccggatg cccggtccac aaggtttgtc gcgtcagcgg gtagagtttt
    20761 tccgggatgc catcttcctg ccagcgacga acgatttcca tcgcccggcg atccgaggac
    20821 aaaatggcgt taccgagcgg cttgtcgttt ttatccagca aaaacaagcc ctttccctgt
    20881 gcggagatgc cgataccgac aatttgttcc ccgctaacac cagaatgagt aagcagggcg
    20941 cgaatgacag ccatgcagca ttgccacagt tctgccatat cgcgctctgc ccaacctggc
    21001 tgcgggctta atgcgcacag cggcaggcgc tgcacgcctg cctcccggcc ttcgcggtca
    21061 tacagcccgg ctttcagcca gctaccgcca caatctaacc ccagccagta ttgcgtcatg
    21121 gtttattgca cctcatccac ggctttcagc agctcagcgc cctgcggaac atctttaaca
    21181 aacatgtcgc ggacctgagt acccagtgcg tcgctaaagg ctttgcggtc gagatcggtg
    21241 atgacttcca cgcccgcttc tttcatgccg tcgatgattt tttgctgatc ttcggcaacc
    21301 agtttgcgct ggtagttacc cgcttcctgt gcagatgaaa ctagcgcctg ctggaactcc
    21361 gggcttaagc catcaaactt cgctttgttg atcaccacca gaagtggcga ataggcgtgg
    21421 tgcgtcagag aaaggtactt ctgtacttcg aaaaattttg ctgaccagac gacgttgatc
    21481 gggtgttcct gagcgtcgat agtgcgcgtt tccagcccgg tatagacttc ggcaaacggc
    21541 atcgggatcg ggttagcgcc aaagactttg aatgcggcga tattcatcgg gctattgttg
    21601 gtgcgaattt tcagcccttt caggtcggcg ggggttttaa ccggtgcgcg cgagttggtg
    21661 acatcgcgcc agccgttttc ccagtaggcc agtactttta gtcctttacc ttcaagtgag
    21721 gctttcagat catcaccgac tttgccgtcg agcgttttat gcgcgtgagc ggtatcgcgg
    21781 aacaggaaag ggacatcaag caagttcatc actggtgata acccggcaaa gttattcgag
    21841 ccggacattt ccatatcgat ggtgccgcca cgtacgccgc tgatcatcgc ctgtgcgtta
    21901 ccgagagtac tgtccgggaa cagttttaat ttcagctcgc ctttggttct ctcctgcaat
    21961 aaatcattga attttttcgc cgcaatatgt tgcgagtcgg tttgtgaggt ttcataaccg
    22021 aaacgtaaag attgcgccgc cagtgaagat gtactaaatg cagccaggcc agcaatgaat
    22081 aatgcgtagg ttacagagcg taatttcata atattttcct tttaattaat ccatttcaaa
    22141 gggaggatga tgagatcggg aataaaaaca aatacgacta ataatgaata taaaaccagg
    22201 acgtaaggga aaacgcctct gactgcatca tcgaatttga gttttgccac ccctgaaata
    22261 acgttaagca cattaccgat aggcggtgta attaagccga ttgagcagtt aatgataaac
    22321 atgacaccga aataaatcgg atcgattcct gcctctttaa ctaaaggcat taataccggg
    22381 gtaagaatta ataccgtcgg cgttaaatcc atgaccatgc cgacaatcag gatagccacc
    22441 ataatgacga taaacagcag acgcggtgaa tcgaccagcg gttgcagtaa atcagaaacc
    22501 atcatcggca gttcagcaat ggtgatcagc caggcggaaa cttgtgccga ggctaccaga
    22561 aacatcacca ctgaggtcgt tttcgctgcg ccaatgagca catgccaaag tgtggcgaag
    22621 gtcatttcac ggtaaataac tgtggcgaca aacagcgcat agaaagcagc aacggccccg
    22681 gcttcggtag gggtaaacag accggagcga aacccgccaa taatgatgac tggaagaaac
    22741 agcgcccaga taccggagac aaaagagtgc cagatttcct gcatcgttgc tttttgctgg
    22801 cggggtaaat taaggcgact ggcctgccac caccaggtaa gcatcagcgt tgcgcccatc
    22861 atcatgccag gggcaatgcc cgccataaac agcttactga tggataatcc gctggaaacg
    22921 ccgaaaataa taaacggaat ggaaggggga ataattggcg cgataatgcc gccagaggca
    22981 atcagccccg cagcccggtt gaccgggtag ttagcgctgc gcatcatcgg cactaatagc
    23041 gcggcaacgg cggcggtatc agcaacagca gaaccagaaa ggctcgccat aatcatagcc
    23101 gccagcacgc cgacgtagcc caacccgcca ggtttatgcc ccaccagttt catcggcagg
    23161 tcaacaatgc gttttgacaa gccgcccgca ttcatgattt cacccgccag cacaaagaac
    23221 ggaatcgcca gcagggagaa gctgtcggct ccgttcacca gcgtttgtgc catgatctgg
    23281 acatcaaaca tgtccagcca gaacattaac gccgctccgc acaacaacag tgcccaggca
    23341 ataggcaaac cgatagcaat accacccaac agacagccca gaaaaatcag cacagccatg
    23401 attaagctcc ttgcggtggc gacgttagag aattgctacg cgtgatgagt tgatataaat
    23461 gacgcagttc aaagaatgcg ataacgaggc tggtgggcag acaagcggca tacatcaggc
    23521 cgatgggcaa accgaggatc ggtgaataat cgctccagtc ctgaattgtt tttagcgttg
    23581 cgccccaggc cagtgcgcca caaataaata agattaagga atgggtaacc agagcgactc
    23641 gtcgctgcca tgcgggggag agtttctcca ccagaaaggt gacctgaacg tgggcgttat
    23701 ccataaaagc cacaatcgcg ccaataaatg ttaaccagac aaataaataa cgtgacaatt
    23761 catct
//
LOCUS       DAZGQL010000083        22484 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.083, whole genome shotgun sequence.
ACCESSION   DAZGQL010000083 DAZGQL010000000
VERSION     DAZGQL010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22484
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.083"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            506..721
                     /gene="essD"
                     /locus_tag="ACHKIR_003673"
     CDS             506..721
                     /gene="essD"
                     /locus_tag="ACHKIR_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HFO9991353.1"
                     /translation="MKSMDKLTTGVAYGTSAGSAGYWFLQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGE"
     gene            721..1218
                     /gene="rrrD"
                     /locus_tag="ACHKIR_003674"
     CDS             721..1218
                     /gene="rrrD"
                     /locus_tag="ACHKIR_003674"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HFO9991354.1"
                     /translation="MPPSLRKAVAAAIGGGAIAIASVLITGPSGNDGLEGVSYIPYKD
                     IVGVWTVCHGHTGKDIMLGKTYTKAECKALLNKDLATVARQINPYIKVDIPETMRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGKQWKGLMTRREIEREIC
                     LWGQQ"
     gene            1435..1617
                     /gene="rzoD"
                     /locus_tag="ACHKIR_003675"
     CDS             1435..1617
                     /gene="rzoD"
                     /locus_tag="ACHKIR_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage lysis lipoprotein RzoD"
                     /protein_id="HFO9991355.1"
                     /translation="MRKLKMMLCVMMLPLVVVGCTSKQSVSQCVKPPPPPAWIMQPPP
                     DWQTPLNGIISPSERG"
     gene            complement(1708..2001)
                     /gene="iss"
                     /locus_tag="ACHKIR_003676"
     CDS             complement(1708..2001)
                     /gene="iss"
                     /locus_tag="ACHKIR_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="increased serum survival lipoprotein Iss"
                     /protein_id="HFO9991356.1"
                     /translation="MKKMLFSAALAMLITGCAQQTFTVGNKPTAVTPKETITHHFFVS
                     GIGQEKTVDAAKICGGAENVVKTETQQTFVNGFLGFITLGIYTPLEARVYCSQ"
     gene            complement(2362..2556)
                     /gene="ylcI"
                     /locus_tag="ACHKIR_003677"
     CDS             complement(2362..2556)
                     /gene="ylcI"
                     /locus_tag="ACHKIR_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO9991357.1"
                     /translation="MSTKNRTRRTTTRNIRFPNQMIEQINIALEQKGSGNFSAWVIEA
                     CRRRLTSEKRAYTSIQSDDG"
     gene            2946..3491
                     /gene="nohD"
                     /locus_tag="ACHKIR_003678"
     CDS             2946..3491
                     /gene="nohD"
                     /locus_tag="ACHKIR_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HFO9991358.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AVIKWYAERDAEIENEKLRREVEELRQASETDLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSG"
     gene            3466..5391
                     /locus_tag="ACHKIR_003679"
     CDS             3466..5391
                     /locus_tag="ACHKIR_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFO9991359.1"
                     /translation="MNISNSQVNRLRHFVRAGLRSLFRPEPQTAVEWADANYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGKKHRDNTLTMKRFSNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDEDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEHYSAPVPDR
                     VAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLRVDEAINKTYT
                     RRNGAEMSVSRICWDTGGIDPTIVYERSKKHGLFRVIPIKGASVYGKPVASMPRKRNK
                     NGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVHFPNNPDIFDLTEAQQLTAEEQV
                     EKWVDGRKKILWDSKKRRNEALDCFVYALAALRISISRWQLDLSALLASLQEEDGAAT
                     NKKTLADYARALSGEDE"
     gene            5388..5594
                     /locus_tag="ACHKIR_003680"
     CDS             5388..5594
                     /locus_tag="ACHKIR_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019058 - viral life cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail joining protein"
                     /protein_id="HFO9991360.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTATSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            5591..7192
                     /locus_tag="ACHKIR_003681"
     CDS             5591..7192
                     /locus_tag="ACHKIR_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO9991361.1"
                     /translation="MKTSSIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPSES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     EARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTS
                     SSRLFRTQFRMVSPKRISNPNNTGDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQK
                     WTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSQEQRERLTGWIGEIAAYYAAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANES
                     WAYFMGRRKFVASRQASQMFLCWLEEAIVRRVVTLPSKARFSFQEARSAWGNCDWIGS
                     GRMAIDGLKEVQEAVMLIEAGLSTYEKECAKRGDDYQEIFAQQVRETMERRAAGLKPP
                     AWAAAAFESGLRQSTEEEKSDSRAA"
     gene            7173..8492
                     /locus_tag="ACHKIR_003682"
     CDS             7173..8492
                     /locus_tag="ACHKIR_003682"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFO9991362.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISRLT
                     DAVSGDSLTAGEAPAALALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPGDVRETLQSRMDATRRMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAID
                     AGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADVTDVVQAT
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPGMTVETAR
                     RILAAAPQSAQARSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTPV"
     gene            8502..8834
                     /locus_tag="ACHKIR_003683"
     CDS             8502..8834
                     /locus_tag="ACHKIR_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFO9991363.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKAPAMTPLMLDTST
                     RKLVAWDGTTDGTAVGILAVDADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTAF
                     AGTAISIV"
     gene            8890..9915
                     /locus_tag="ACHKIR_003684"
     CDS             8890..9915
                     /locus_tag="ACHKIR_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFO9991364.1"
                     /translation="MSMYTTAQLLAANEQKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLA
                     DPAYRRRRIIMQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSAAN
                     NITQSGGTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVKEKL
                     DTRRGSHSELETAVKDLGEAVSYKGMYGDTAIVVYSGQYVENDVKKNFLPDNTMVLGN
                     TQARGLRTYGCIQDADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDA
                     FVSVQLA"
     gene            9957..10352
                     /locus_tag="ACHKIR_003685"
     CDS             9957..10352
                     /locus_tag="ACHKIR_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFO9991365.1"
                     /translation="MTKDELIARLQVLGEQLNRDVSLTGTKEELVLRVAELEEELDDT
                     DDAAGQDTSVSPENALTGHENEVVSAQPDTVIDTAALVTVVALVTLHTDALHATRDEP
                     VAFVLPGTAFRVSAGVAAEMTEHGLARMQ"
     gene            10364..10717
                     /locus_tag="ACHKIR_003686"
     CDS             10364..10717
                     /locus_tag="ACHKIR_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFO9991366.1"
                     /translation="MADSDNLFDAAIARADETIRGYMGTSATMTSGELSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDDVRQLRRGDTLTIGEENFWVDRISPDDGGSCH
                     LWLGRGVPPAVNRRR"
     gene            10729..11307
                     /locus_tag="ACHKIR_003687"
     CDS             10729..11307
                     /locus_tag="ACHKIR_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9991367.1"
                     /translation="MAIKGLEQAVENLSRISKTAVPGAAAMAINRVASSAISQSASQV
                     ARETKVRRKLVKERARLKRATVKNPQARIRVNRGDLPVIKLGNARVVLSRRRRRKKGQ
                     RSSLKGGGSVLVVGNRRISGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            11304..11699
                     /locus_tag="ACHKIR_003688"
     CDS             11304..11699
                     /locus_tag="ACHKIR_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFO9991368.1"
                     /translation="MKHTELRAAVLDALEKHDTGATFFDGRPAVFDEADFPAVAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDAWMESRIYPVMSDIPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVITYEM"
     gene            11707..12447
                     /locus_tag="ACHKIR_003689"
     CDS             11707..12447
                     /locus_tag="ACHKIR_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9991369.1"
                     /translation="MPVPNPVMPVKGAGTTLWVYKGSGDPYANPLSDVDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     TTGMTVTPASTSVVKGQSTTLTVAFQPEGATDKSFRAVSADKTKATVSVSGMTITVKG
                     VAAGKVNIPVVSGNGELAAVAEITVTAS"
     gene            12463..12885
                     /locus_tag="ACHKIR_003690"
     CDS             12463..12885
                     /locus_tag="ACHKIR_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFO9991370.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLALMKRQAEQAESDSN
                     RKFTVEDAIRTGAFLVAMSLWHNHPQKTQMPSMNEAVKQIEQEVLTTWPTEAISHAEN
                     VVYRLSGMYEFVVNNAPEQTEDAGPAEPVSAGKCSTVS"
     gene            12867..13301
                     /locus_tag="ACHKIR_003691"
     CDS             12867..13301
                     /locus_tag="ACHKIR_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFO9991371.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPDMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPASPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            13294..15873
                     /locus_tag="ACHKIR_003692"
     CDS             13294..15873
                     /locus_tag="ACHKIR_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO9991372.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTETDAKKTAAVV
                     EQSLSRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQSPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQA
                     GLTADRMLVLSRAGQAAGLTFNQTSESLTALVNAGVRGGEQFEAISQSVARFSSASGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGDEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARAFKSMWDSVLDIGRPDTAQGMLEKAEKAFDE
                     ADKKWQWYQSRSHRRGKTSAFLANLRGAWEDRANAQLGLSAATLQADLEKAREMAAKD
                     WAESEASRLKYTEEAQKAYERLQTPLEKYTARQEELNKALKDGKILQADYNTLMAAAK
                     KDYEATLKKPKQSGVKVSAGDRQEDSAHAALLTLQAELRTLEKHAGANEKISQQRRDL
                     WKAESQFAVLEEAAQRRQLSAQEKSLLAHKDETLEYKRQLAALGDKVTYQERLNALAQ
                     QADKFAQQQRAKRAAIDAKSRGLTDRQAEREATEQRLKEQYGDNPLALNNVMSEQKKT
                     WAAEDQLRGSWMAGLKSGWSEWEESATDSMSQVKSAATQTFDGIAQNMAAMLTGSEQN
                     WRSFTRSVLSMMTEILLKQAMVGIVGSIGSAIGGAVGGGASASGGTAIQAAAAKFHFA
                     TGGFTGTGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYATGGYVGTPGSMAD
                     SRSQASGTFEQNNHVVINNDGTNGQIGPAALKAVYDMARKGARDEIQTQMRDGGLFSG
                     GGR"
     gene            15870..16199
                     /locus_tag="ACHKIR_003693"
     CDS             15870..16199
                     /locus_tag="ACHKIR_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9991373.1"
                     /translation="MKTFRWKVKPGMDVASVPSVRKVRFGDGYSQRAPAGLNANLKTY
                     SVTLSVPREEATVLESFLEEHGGWKSFLWTPPYEWRQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVN"
     gene            16199..16897
                     /locus_tag="ACHKIR_003694"
     CDS             16199..16897
                     /locus_tag="ACHKIR_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO9991374.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQPYPIQGSGFELNGKGTSTRPTLTVSNLYGMVTGMAEDMQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYHGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            16903..17646
                     /locus_tag="ACHKIR_003695"
     CDS             16903..17646
                     /locus_tag="ACHKIR_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO9991375.1"
                     /translation="MTETESAILAHARRCAPAESCGFVVRTPEGERYFPCVNISGEPE
                     AYFRMSPEDWLRAEMQGEIVALVHSHPGGLPWLSETDRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLSGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHREDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDSELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHREWHASAFTGICNDLAAASTFV"
     gene            17643..18215
                     /locus_tag="ACHKIR_003696"
     CDS             17643..18215
                     /locus_tag="ACHKIR_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO9991376.1"
                     /translation="MKTGAEAIRALATQLPAFRQKLSDGWYQVRIAGRDVSTSGLTAQ
                     LHETLPDGAVIHIVPRVAGAKSGGVFQIVLGAAAIAGSFFTAGATLAAWGAAIGAGGM
                     TGILFSLGASMVLGGVAQMLAPKARTPRTQTTDNGKQNTYFSSLDNMVAQGNVLPVLY
                     GEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            18275..21772
                     /locus_tag="ACHKIR_003697"
     CDS             18275..21772
                     /locus_tag="ACHKIR_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFO9991377.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPIEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITW
                     TITSANIDRLRVTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVGNLPPRPFSIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTFKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQNCDQSVPDGFGGTEPRITCNAWLTTQRK
                     AWDVLSDFCSAMRCMPIWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPDNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAGYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVTADDGSERLVSTARTTETTYRFTQ
                     LALGNYRLTVRAVNAWGQQGDPASVSFRIAAPAAPSRIELTPGYFQITATPHLAVYDP
                     TVQFEFWFSEKRIADIRQVETSARYLGTALYWIAASINIKPGHDYYFYVRSVNTVGKS
                     AFVEAVGQPSDDASGYLDFFKGEIGKTHLAQELWTQIDNGQLAPDLTEIRTSITDVSN
                     EITQTVNKKLEDQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMQDGRLYIAGIGAGIEN
                     TPDGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNEVFLKRLTAPTITSGGNP
                     PAFSLTSDGRLTAKNADISGSVNANSGTLNNVTINQNCTIKGMLEATQVRGDFVKAVS
                     KAFPKKVGTWGNTETPNGTVTVTISDDHNFDRQIIIPPIIFNGIAYDDPGSGNNPGGT
                     RYTGYGFEVRKNGVLIASRETKGAIPGSYSAVIDMPSGRGSVTLEFKIFQKGNQGAGN
                     ITDCTVIVTKKAASGISIR"
     gene            21843..22442
                     /locus_tag="ACHKIR_003698"
     CDS             21843..22442
                     /locus_tag="ACHKIR_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family protein"
                     /protein_id="HFO9991378.1"
                     /translation="MRKVCAAILSAAICLAVSGAPAWASEHQSTLSAGYLHASTNVPG
                     SDDLNGINVKYRYEFTDTLGLVTSFSYAGDKNRQLTRYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKKHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVAIDIAYEGSGSGDWRTDGFIVGVGYKF"
BASE COUNT         5220 a         5576 c         6864 g         4824 t
ORIGIN      
        1 atgtaagttt tggtttctcg tttgatttgt gaagtagatc tctattttta tctgaacttt
       61 tttctatcga atcctattca tggatcttgg ctgaataaaa ataaatctat tagccaattt
      121 atattaacgg ctgttattta taagtgctct gtaatttgaa ggttcaattt aaatcggcta
      181 aaaataacac cggaaattat ttgttggtta tttgttgaga tttgcttatg tatttgtagt
      241 ggtgttttca atactcggta gcattctcgc aaatatcatt ttgtggttta cgtacgtaaa
      301 aaattggtta tgctgttaag agtggttact tcgtcacaca gcttaaaccc gccgtcgagc
      361 gggtttttcc attttttgag tctcgatatt agctgataac ccaatacctg agttattcac
      421 tgactccgag tctgttacgt ttctgctttt ttgcgatacg ttgtattccc tcaatttaca
      481 cccgctttgt ctgcgaggtg gggttatgaa atccatggat aagttaacaa cgggtgtcgc
      541 ctatggcacc tcagcaggta gtgccggtta ctggttttta cagctgctcg ataaagtcac
      601 gccctcacag tgggcagcaa taggtgtgct gggtagcctg gtatttggcc tgctgacgta
      661 cctgacaaac ctttatttca agattaaaga agataagcgc aaggctgcga gaggtgaata
      721 atgcctccat cattacgaaa agccgttgct gctgctattg gtggcggagc aattgctata
      781 gcatcagtgt taatcactgg cccaagtggt aacgatggtc tggaaggtgt cagctacata
      841 ccatacaaag atattgttgg tgtatggact gtatgtcacg ggcatacagg aaaagacatc
      901 atgctcggta aaacgtatac caaagcagaa tgcaaagccc tcctgaataa agaccttgcc
      961 acggtcgcca gacaaattaa cccgtacatc aaagtcgata taccggaaac aatgcgcggc
     1021 gctctttact cattcgttta caacgtgggt gctggcaatt tcagaacatc gacgcttctt
     1081 cgcaaaataa accagggcga tatcaaaggc gcatgtgatc agctacgtcg ctggacatat
     1141 gctggcggta agcaatggaa aggtctcatg actcgtcgtg agattgagcg tgaaatctgt
     1201 ttgtggggtc agcaatgaac agagtaaccg cgattatctc cgctctggtt atctgcatca
     1261 tcgtctgcct gtcatgggct gttaatcatt accgtgataa cgccattacc tacaaagccc
     1321 agcgcgacaa aaatgccaga gaactgaagc tggcgaacgc ggcaattact gacatgcaga
     1381 tgcgtcagcg tgatgttgct gcgctcgatg caaaatacac gaaggagtta gctgatgcga
     1441 aagctgaaaa tgatgctctg cgtgatgatg ttgccgctgg tcgtcgtcgg ttgcacatca
     1501 aagcagtctg tcagtcagtg cgtgaagcca ccaccgcctc cggcgtggat aatgcagcct
     1561 ccccccgact ggcagacacc gctgaacggg attatttcac cctcagagag aggctgatca
     1621 ctatgcaaaa acaactggaa ggaacccaga agtatattaa tgagcagtgc agatagagct
     1681 gcccatatcg atgggcaact catgcaatta ttgtgagcaa tacacacgcg cttccagcgg
     1741 agtataaatg cctaaagtaa taaaaccgag aaatccattt acgaatgttt gctgggtttc
     1801 tgttttaaca acattttctg cgccgccaca aattttggct gcatcaacag ttttctcctg
     1861 tccaattccc gaaacgaaga aatgatgggt gatggtttcc tttggtgtta ctgctgtcgg
     1921 tttgtttcca acagtaaacg tctgttgagc acatcctgta ataagcattg ccagagcggc
     1981 agaaaacaac atttttttca tcttattatc ctgcattgtt aaaaacggca gaatcctatg
     2041 tgacaacaat taaacgatag ttaaatggat tgatgaaaat taaaactata taggtggatg
     2101 ctcagcctat tggaggaggg ggggcactca gaatcctgtg gaatgaaata aaccgctcta
     2161 tctgtccatt acccttttag ctgcgctgta tcgtcgccgt attcccgcat taaccatgac
     2221 cgtagcccga cggggaattc cttctgcgtg agtgtgcggg aataatcaaa aacgatgcac
     2281 accgggtttt actgtgctga cagacgcagg gttaccctca tagtcgcttt tccggtgcga
     2341 tggtggaaga aaccgggatg ttcatccatc atcactttgg attgatgtat atgctctctt
     2401 ttctgacgtt agtctccgac ggcaggcttc aatgacccag gctgagaaat tcccagaccc
     2461 tttttgctca agagcgatgt taatttgttc aatcatttgg ttaggaaagc ggatgttgcg
     2521 ggttgttgtt ctgcgggttc tgttcttcgt tgacatgagg ttgccccgta ttcagtgtcg
     2581 ctgatttgta ttgtctgaag ttgtttttac gttaagttga tgcagatcaa ttaatacgat
     2641 acctgcgtca taattgatta tttgacgtgg tttgatggcg tagatgcacg ttgtgacatg
     2701 tagatgataa ttattatcat ttttgcgggt cctttccggc gatccgacag gttacggggc
     2761 ggcgacctcg cgggttttcg ctatttatga aaattttccg gtttaaggcg tttccgttct
     2821 tcttcgtcat aacttaatgt ttttatttaa aataccctct gaaaagaaag gaaacgacag
     2881 gtgctgaaag cgagcttttt ggcctctgtc gtttcctttc tctgtttttg tccgtggaat
     2941 gaacaatgga agtcaacaaa aagcagctgg ctgacatttt cggtgcgagt atccgtacca
     3001 ttcagaactg gcaggaacag ggaatgcccg ttctgcgagg cggtggcaag ggtaatgagg
     3061 tgctttatga ctctgccgcc gtcataaaat ggtatgccga aagggatgct gaaattgaga
     3121 acgaaaagct gcgccgggaa gttgaagaac tgcggcaggc cagcgagaca gatctccagc
     3181 cagggactat tgagtacgaa cgccatcgac ttacgcgtgc gcaggccgac gcacaggagc
     3241 tgaaaaatgc cagagactcc gctgaagtgg tggaaaccgc attctgtact ttcgtgctgt
     3301 cgcggatcgc aggtgaaatt gccagtattc tcgacgggat ccccctgtcg gtgcagcggc
     3361 gttttccgga actggaaaac cgacatgttg atttcctgaa acgggatatc atcaaagcca
     3421 tgaacaaagc agccgcgctg gatgaactga taccggggtt gctgagtgaa tatatcgaac
     3481 agtcaggtta acaggctgcg gcattttgtc cgcgccgggc ttcgctcact gttcaggccg
     3541 gagccacaga ccgccgttga atgggcggat gccaattact atctcccaaa agaatccgca
     3601 taccaggaag ggcgctggga aacactgccc tttcagcggg ccatcatgaa tgcgatgggc
     3661 agcgactaca tccgtgaggt gaatgtggtg aagtctgccc gtgtcggtta ttccaaaatg
     3721 ctgctgggtg tttatgccta ctttatagag cataagcagc gcaacaccct tatctggttg
     3781 ccgacggatg gtgatgccga gaactttatg aaaacccacg ttgagccgac catccgcgat
     3841 attccgtcgc tgctggcgct ggctccgtgg tatggcaaaa agcaccggga taacacgctc
     3901 actatgaagc gtttttccaa tggtcgtggc ttctggtgcc tgggcggtaa agcggcaaaa
     3961 aactaccgtg aaaagtcggt ggatgtggcg ggttatgatg aacttgctgc ctttgatgag
     4021 gatattgaac aggaaggctc tccgacgttc cttggcgaca aacgtattga aggctcggtc
     4081 tggccaaagt ccatccgtgg ctccacgccc aaagtgagag gcacctgcca gattgagcgt
     4141 gcagccagtg aatccccgca ttttatgcgt tttcatgttg cctgcccgca ctgcggggag
     4201 gagcagtatc ttaaatttgg cgacaaagag acgccgtttg gcctcaaatg gacgccggat
     4261 gacccctcca gcgtgtttta tctctgcgag cataatgcct gcgtcatccg ccagcaggag
     4321 ctggacttta ctgatgcccg ttatatctgc gaaaagaccg ggatctggac ccgtgatggc
     4381 attctctggt tttcgtcatc cggtgaagag attgagccgc cggacagtgt gacctttcac
     4441 atctggacgg cgtacagccc gttcaccacc tgggtgcaga ttgtcaaaga ctggatgaag
     4501 acgaaagggg atacgggaaa acgtaaaacc ttcgtgaaca ccacgctcgg tgagacatgg
     4561 gaagcgaaaa ttggcgaacg tccggatgct gaggtgatgg cggagcggaa agagcattat
     4621 tcagcgcccg ttcctgaccg tgtggcttac ctgaccgccg gtatcgactc ccagctggac
     4681 cgctacgaaa tgcgcgtatg gggatggggg ccgggtgagg aaagctggct gattgaccgg
     4741 cagattatta tgggccgcca cgacgatgaa cagacgctgc tgcgtgtgga tgaggccatc
     4801 aataaaacct atacccgccg gaatggtgca gaaatgtcgg tatcccgtat ctgctgggat
     4861 actggcggga ttgacccgac cattgtgtat gaacgctcga aaaagcatgg gctgttccgg
     4921 gtgatcccca ttaaaggggc atccgtctac ggaaagccag tggccagcat gccacgtaag
     4981 cgaaacaaaa acggggttta ccttaccgaa atcggtacgg ataccgcgaa agagcagatt
     5041 tataaccgct tcacactgac gccggaaggg gatgaaccgc ttcccggtgc cgttcacttc
     5101 ccgaataacc cggatatttt tgatctgacc gaagcgcagc agctgactgc tgaagagcag
     5161 gtcgaaaaat gggtggatgg caggaaaaaa atactgtggg acagcaaaaa gcgacgcaat
     5221 gaggcgctcg actgcttcgt ttatgcgctg gcggcgctgc gcatcagtat ttcccgctgg
     5281 cagctggatc tcagtgcact gctggcgagc ctgcaggaag aggatggtgc agcaaccaac
     5341 aagaaaacac tggcagatta cgcccgtgcc ttatccggag aggatgaatg acgcgacagg
     5401 aagaacttgc cgctgcccgt gcggcactgc atgacctgat gacaggaaaa cgggtggcaa
     5461 cggtacagaa agacggacgg cgagtggagt ttacggccac ttccgtgtct gacctgaaaa
     5521 aatacattgc ggagctggaa gtgcagaccg gcatgacaca gcgacgcagg ggacctgcag
     5581 gattttatgt atgaaaacgt cctccattcc cacccttctg gggccggacg gcatgacatc
     5641 gctgcgtgaa tatgccggtt atcacggcgg tggcagcgga tttggtgggc agttgcgggc
     5701 gtggaaccca ccgagtgaaa gtgtggatgc agccctgttg cccaacttta cccgtggcaa
     5761 tgcccgcgcg gacgatctgg tacgcaataa cggctatgcc gccaacgcca tccagctgca
     5821 tcaggatcat atcgtcgggt cttttttccg gctcagtcat cgcccaagct ggcgctatct
     5881 gggcatcggg gaggaagaag cccgtgcctt ttcccgcgag gttgaagcgg catggaaaga
     5941 gtttgccgag gatgactgct gctgcattga cgttgagcga aaacgcacgt ttaccatgat
     6001 gattcgggaa ggtgtggcca tgcacgcctt taacggtgaa ctgttcgttc aggccacctg
     6061 ggataccagt tcgtcgcggc ttttccggac acagttccgg atggtcagcc cgaagcgcat
     6121 cagcaacccg aacaataccg gcgacagccg gaactgccgt gccggtgtgc agattaatga
     6181 cagcggtgcg gcgctgggat attacgtcag cgaggacggg tatcctggct ggatgccgca
     6241 gaaatggaca tggatacccc gtgagttacc cggcgggcgt gcctcgttca ttcacgtttt
     6301 tgaacccgtg gaggacgggc agacccgcgg tgcaaatgtg ttttacagcg tgatggagca
     6361 gatgaagatg ctcgacacgc tgcagaacac gcagctgcag agcgccattg tgaaggcgat
     6421 gtatgccgcc accattgaga gtgagctgga tacgcagtca gcgatggatt ttattctggg
     6481 cgcgaacagt caggagcagc gggaaaggct gaccggctgg attggtgaaa ttgccgcgta
     6541 ttacgccgca gcaccggtcc gtctgggagg cgcaaaagtg ccgcacctga tgccggggga
     6601 ctcactgaac ctgcagacgg ctcaggacac ggataacggc tactccgtgt ttgaacagtc
     6661 actgttgcgg tatatcgctg ccgggctggg tgtctcgtat gagcagcttt cccggaatta
     6721 cgcccagatg agctactcca cggcacgggc cagtgcgaac gagtcgtggg cgtactttat
     6781 ggggcggcga aaattcgtcg catcccgtca ggcgagccag atgtttctgt gctggctgga
     6841 agaggccatc gttcgccgcg tggtgacgtt accttcaaaa gcgcgtttca gctttcagga
     6901 agcccgcagc gcctggggga actgtgactg gataggctcc ggtcgtatgg ccatcgatgg
     6961 tctgaaagaa gtacaggaag cggtgatgct gatagaagcc ggactgagta cctacgagaa
     7021 agagtgcgca aaacgcggtg acgactatca ggaaattttt gcccagcagg tccgtgaaac
     7081 gatggagcgc cgtgcagccg gtcttaaacc gcccgcctgg gcggctgcgg catttgaatc
     7141 cggactgcga caatcaacag aggaggagaa gagtgacagc agagctgcgt aatctcccgc
     7201 atattgccag catggctttt aatgagccgc tgatgcttga acccgcctat gcgcgggttt
     7261 tcttttgtgc gcttgcaggc cagcttggga tcagtcgcct gacggatgca gtatccggcg
     7321 acagcctgac tgccggagag gcacccgcgg cgctggcgtt atccggtgat gatgacggac
     7381 cacgacaggc ccgcagttat caggtcatga acggcatcgc cgtgctgccg gtgtccggta
     7441 cgctggtcag ccggacgcgg gcgctgcagc cgtattcggg aatgaccggt tacaacggca
     7501 ttatcgcccg tctgcaacag gctgccagcg atccgatggt ggacggcatt ctgctcgata
     7561 tggacacacc gggcgggatg gtggcgggag catttgactg tgctgacatc atcgcccgtg
     7621 tgcgtgacat aaagccggta tgggcgctgg ccaacgacat gaactgcagt gcaggtcagc
     7681 tgcttgccag cgccgcctcc cggcgtctgg tcacgcagac cgcccggaca ggctccatcg
     7741 gcgtcatgat ggctcacagt aattacggcg ctgcgctgga gaaacagggc gtggaaatca
     7801 cgctgattta cagcggcagc cataaggtgg atggcaaccc ctacagccat ctaccgggtg
     7861 atgtccggga gacactgcag tcccggatgg atgcaacccg ccggatgttt gcgcagaagg
     7921 tgtcggcata taccggcctg tccgtgcagg ctgtgctgga taccgaggct gcagtgtaca
     7981 gcggtcagga ggccattgat gccggactgg ctgatgaact tgtcaacagc accgatgcga
     8041 tcaccgttat gcgtgatgca ctggatgcac gtaaatcccg tctctcagga gggcgaatga
     8101 ccaaagagac tcaatcaaca actgtttcag ccactgcttc gcaggctgac gttactgacg
     8161 tggtgcaagc gacggagggc gaaaacgcca gcgcggcgca gccggacgtg aacgcgcaga
     8221 tcaccgctgc ggttgcggca gaaaacagcc gcattatggg gatcctcaac tgtgaggaag
     8281 ctcacggacg cgaagaacag gcacgtgtgc tggccgaaac ccccggtatg accgtggaaa
     8341 cggcccgccg cattctggca gctgcaccac agagtgcaca ggcgcgcagt gacactgcgc
     8401 tggatcgtct gatgcagggg gcaccggcac cactggctgc aggtaacccg gcatctgatg
     8461 ccgttaacga tttgctgaac acaccagtgt aagggatgtt tatgacgagc aaagaaacct
     8521 ttacccatta ccagccgctg ggcaacagtg acccggcaca tacggcaacc gcgcccggcg
     8581 gattgagtgc gaaagcgcct gcaatgaccc cgctgatgct ggacacctcc acccgtaagc
     8641 tggttgcgtg ggatggcacc accgacggta ctgccgttgg cattctggcg gttgatgctg
     8701 accagaccag caccacgctg acgttctaca agtccggcac gttccgttat gaggatgtgc
     8761 tctggccgga ggctgccagc gacgagacga aaaaacggac cgcgtttgcc ggaacggcaa
     8821 tcagcatcgt ttaaccttac ccttcatcac taaaggccgc ctgtgcggct ttttttacgg
     8881 gattttttta tgtcgatgta cacaaccgcc cagctgctgg cggcaaatga gcagaaattt
     8941 aagtttgatc cgctgtttct gcgtctcttt ttccgtgaga gctatccctt caccacggag
     9001 aaagtctatc tctcacaaat tccgggactg gtaaacatgg cgctgtacgt ttcgccgatt
     9061 gtttccggtg aggttatccg ttcccgtggc ggctccacct ctgaatttac gccgggatat
     9121 gtcaaaccca agcatgaggt gaatccgcag atgaccctgc gtcgcctgcc ggatgaagat
     9181 ccgcagaatc tggcggaccc ggcttaccgc cgccgtcgca tcatcatgca gaacatgcga
     9241 gacgaagagc tggccattgc tcaggtcgaa gagatgcagg cagtttctgc tgtgcttaag
     9301 ggcaaataca ccatgaccgg tgaagccttc gatccggttg aggtggatat gggccgcagt
     9361 gcggcgaaca acatcacaca gtccggtggt acggagtgga gcaagcgtga caagtccacg
     9421 tatgacccga ccgacgatat cgaagcctat gcgctgaacg ccagcggcgt ggtgaatatc
     9481 atcgtgtttg atccgaaagg ctgggcgctg ttccgttcct tcaaagccgt caaggagaag
     9541 ctggataccc gtcgcggctc tcattccgag ctggagacag cggtaaaaga cctgggcgaa
     9601 gcggtgtcct ataaggggat gtatggcgat acggcgatcg tcgtgtattc cggacagtac
     9661 gtggaaaacg acgtcaaaaa gaacttcctg ccggacaaca cgatggtgct ggggaacact
     9721 caggcacgcg gtctgcgcac ctatggctgc attcaggatg cggacgcaca gcgcgaaggt
     9781 attaacgcct ctgcccgcta cccgaaaaac tgggtgacca ccggcgatcc ggcgcgtgag
     9841 ttcaccatga ttcagtcagc accgctgatg ctgctggctg atcctgatgc gttcgtgtcc
     9901 gtacaactgg cgtaatcatg gcccttcggg gccattttct ctctgtggag gagtccatga
     9961 cgaaagatga actgattgcc cgtcttcagg tgctgggtga gcaactgaac cgtgatgtca
    10021 gcctgacggg gacgaaagaa gaactggtgc tccgtgtggc agagctggaa gaggagcttg
    10081 atgacacgga tgacgctgcc ggtcaggaca catctgtcag cccggaaaat gcgctgaccg
    10141 gacatgaaaa tgaggtggta tcagcgcagc cggataccgt gattgatacg gctgctctgg
    10201 tcacggtcgt ggcactggtg acgctgcata ctgatgcact tcacgccacg cgggatgagc
    10261 ctgtggcatt tgtgctgccg ggaacggcgt ttcgtgtctc tgccggtgtg gcagccgaaa
    10321 tgacagaaca tggcctggcc agaatgcaat aacgggaggc gctgtggctg attccgataa
    10381 cctgttcgat gctgccattg cccgcgccga tgaaacgata cgcgggtaca tgggaacgtc
    10441 agccaccatg acatccggtg agctgtccgg tgctgtgata cgtggtgttt ttgatgaccc
    10501 tgaaaatatc agctatgccg gacagggggt gcgcgttgaa ggctccagcc cgtccctgtt
    10561 tgtccggact gatgatgtgc ggcagctgcg gcgtggagac acactgacca tcggcgagga
    10621 aaacttctgg gtggaccgga tttcgccgga tgatggcgga agctgtcatc tctggcttgg
    10681 gcgtggcgtg ccgcctgccg ttaaccgtcg ccgctgaaag ggggatgtat ggccataaaa
    10741 ggtcttgagc aggccgttga aaacctcagc cgtatcagca aaacggcggt gcctggtgcc
    10801 gccgcaatgg ccattaaccg cgttgcgtca tccgcgatat cgcagtctgc gtcacaggtt
    10861 gcccgtgaga caaaggtacg ccggaaactg gtaaaggaaa gggccaggct gaaaagggcc
    10921 acggtcaaaa atccgcaggc cagaatcagg gttaaccggg gggatttgcc cgtaatcaag
    10981 ctgggtaacg cgcgggttgt cctttcccgc cgcagacgtc gtaaaaaggg gcagcgttca
    11041 tccctgaaag gtggcggcag cgtgcttgtg gtgggaaacc gtcgtatttc cggcgcgttt
    11101 attcagcaac tgaaaaatgg ccggtggcat gtcatgcagc gtgtggccgg gaaaaaccgt
    11161 taccccattg atgtggtgaa aatcccgatg gcggtgccgc tgaccacggc gtttaaacag
    11221 aatattgaac ggatacggcg tgaacgtctt ccgaaagagc tgggctatgc gctgcagcat
    11281 caactgagaa tggtaataaa gcgatgaaac atactgaact ccgtgcagcc gtactggatg
    11341 cactggagaa gcatgacacc ggggcgacgt tttttgatgg tcgccccgct gtttttgatg
    11401 aggcggattt tccggcagtt gccgtttatc tcaccggcgc tgaatacacg ggcgaagagc
    11461 tggacagcga tacatggcag gcggagctgc atattgaagt tttcctgcct gctcaggtgc
    11521 cggattcaga gctggatgcg tggatggagt cccggattta tccggtgatg agtgatatcc
    11581 cggcactgtc agatttgatc accagtatgg tggccagcgg ctatgactac cggcgcgacg
    11641 atgatgcggg cctgtggagt tcagccgatc tgacttatgt cattacctat gaaatgtgag
    11701 gacgctatgc ctgtaccaaa tcctgtaatg ccggtgaaag gtgccgggac caccctgtgg
    11761 gtttataagg gaagcggtga cccttatgcg aatccgcttt cagacgttga ctggtcgcgt
    11821 ctggcaaaag ttaaagacct gacgcccggc gaactgaccg ctgagtccta tgacgacagc
    11881 tatctcgatg atgaagatgc agactggact gcgaccgggc aggggcagaa atctgccgga
    11941 gataccagct tcacgctggc gtggatgccc ggagagcagg ggcagcaggc gctgctggcg
    12001 tggtttaatg aaggggatac ccgagcctat aaaatccgct tcccgaacgg cacggtcgat
    12061 gtgttccgcg gctgggtcag cagtatcggt aaggcggtga cggcgaagga agtgatcacc
    12121 cgcacggtga aagtcaccaa cgtgggccgt ccgtcgatgg cagaagatcg cagcacggtg
    12181 acggcgacaa ccggcatgac tgtgacgcct gccagcacct cggtggtgaa agggcagagc
    12241 accacgctga ccgtggcatt ccagccggaa ggcgcaaccg acaagagctt ccgtgcggtg
    12301 tctgcggata aaacaaaagc caccgtgtcg gtcagtggta tgaccatcac cgtgaaaggt
    12361 gttgctgcag gcaaggtcaa cattccggtc gtatccggta atggtgaact tgctgcggtt
    12421 gcagaaatca ccgtcaccgc cagttaatcc ggggagtcag cgatgttcct gaaaaccgaa
    12481 tcatttgaac ataacggtgt gaccgtcacg ctttctgaac tgtcagccct gcagcgaatt
    12541 gagcatctcg ccctgatgaa acggcaggca gaacaggcgg agtcagacag caaccggaag
    12601 tttactgtgg aagacgccat cagaaccggc gcgtttctgg tggcgatgtc cctgtggcat
    12661 aaccatccgc agaagacgca gatgccgtcc atgaatgaag ccgttaaaca gattgagcag
    12721 gaagtgctta ccacctggcc cacggaggca atttctcatg ctgaaaacgt ggtgtaccgg
    12781 ctgtctggta tgtatgagtt tgttgtgaat aatgcccctg aacagacaga ggacgccggg
    12841 cccgcagagc ctgtttctgc gggaaagtgt tcgacggtga gctgagtttt gccctgaaac
    12901 tggcgcgaga gatggggcga cccgactggc gtgccatgct tgccgggatg tcatccacgg
    12961 agtatgccga ctggcaccgc ttttacagta cccattattt tcatgatgtt ctgctggata
    13021 tgcacttttc cgggctgacg tacaccgtgc tcagcctgtt tttcagcgat ccggatatgc
    13081 atccgctgga tttcagtctg ctgaaccggc gtgaggctga cgaagagcct gaagatgatg
    13141 tgctgatgca gaaagcggca gggcttgccg gaggcgtccg ctttggcccg gacgggaatg
    13201 aagttatccc cgcttccccg gatgtggcgg acatgacgga ggatgacgta atgctgatga
    13261 cagtatcaga agggatcgca ggaggagtcc ggtatggctg aaccggtagg cgatctggtc
    13321 gttgatttaa gtctggatgc ggccagattt gacgagcaga tggccagagt caggcgtcat
    13381 ttttccggta cggaaactga tgcgaaaaaa acagcggcag tcgttgaaca gtcgctgagc
    13441 cgacaggcgc tggctgcaca gaaagcgggg atttccgtcg ggcagtataa agccgccatg
    13501 cgtatgctgc ctgcacagtt caccgacgtg gccacgcagc ttgcaggcgg gcaaagcccg
    13561 tggctgatcc tgctgcaaca ggggggtcag gttaaggact ccttcggcgg gatgatcccc
    13621 atgttcaggg ggcttgccgg tgcgatcacc ctgccgatgg tgggggccac ctcgctggcg
    13681 gtggcgaccg gtgcgctggc gtatgcctgg tatcagggca actcaaccct gtccgatttc
    13741 aacaaaacgc tggtcctttc cggcaatcag gcgggactga cggcagatcg tatgctggtc
    13801 ctgtccagag ccgggcaggc ggcagggctg acgtttaacc agaccagcga gtcactgacg
    13861 gcgctggtga atgccggtgt gcgtggtggt gagcagtttg aggcgatcag ccagagtgtg
    13921 gcgcgtttct cctctgcatc cggcgtggag gtggacaagg tcgctgaagc cttcgggaag
    13981 ctgaccacag acccgacgtc gggactgaca gcgatggcac gtcagttcca taacgtgacg
    14041 gcggagcaga ttgcgtatgt tgctcagttg cagcgttccg gagatgaagc cggggcattg
    14101 caggcggcga acgaggccgc aacgaaaggg tttgatgacc agacccgccg cctgaaagag
    14161 aacatgggca cgctggagac ctgggcagac aggacagcac gggcattcaa atccatgtgg
    14221 gattcggtgc tggatattgg tcgcccggac actgcccagg gaatgctgga gaaagcagaa
    14281 aaggcttttg atgaggcgga caaaaaatgg cagtggtatc agagccggag ccaccggcgc
    14341 ggtaaaacct cagcatttct tgccaatctc cggggagcat gggaggacag agcgaatgcg
    14401 caacttgggc tttcagccgc cacgttgcag gccgatcttg aaaaggccag agagatggca
    14461 gcaaaggact gggccgagtc tgaggcatca cggctgaaat ataccgaaga ggcgcagaag
    14521 gcttacgaac ggctgcagac gccgctggag aaatataccg cccgtcagga agaactgaac
    14581 aaggcactga aagacgggaa aatcctgcag gcggattaca acacgctgat ggcggcggcg
    14641 aaaaaggatt atgaagcgac gctgaaaaag ccgaaacagt ccggcgtgaa ggtgtctgcg
    14701 ggcgatcgtc aggaagacag tgctcatgct gccctgctga cgcttcaggc tgaactccgg
    14761 acgctggaga agcatgccgg agcaaatgag aaaatcagcc agcagcgccg ggatttgtgg
    14821 aaggcggaga gccagttcgc ggtactggag gaggcggcgc aacgtcgcca gctgtctgca
    14881 caggagaaat ccctgctggc gcataaagat gagacgctgg agtacaaacg ccagctggct
    14941 gcacttggcg acaaggttac gtatcaggag cgcctgaacg cgctggcgca gcaggcggat
    15001 aaattcgcac agcagcaacg ggcaaaacgg gccgccattg atgcgaaaag ccgggggctg
    15061 actgaccggc aggcagaacg ggaagccacg gaacagcgcc tgaaggaaca gtatggcgat
    15121 aatccgctgg cgctgaataa cgtcatgtca gagcagaaaa agacctgggc ggctgaagac
    15181 cagcttcgcg ggagctggat ggcaggcctg aagtccggct ggagtgagtg ggaagagagc
    15241 gccacggaca gtatgtcgca ggtaaaaagt gcagccacgc agacctttga tggtattgca
    15301 cagaatatgg cggcgatgct gaccggcagt gagcagaact ggcgcagctt cacccgttcc
    15361 gtgctgtcca tgatgacaga aattctgctt aagcaggcaa tggtggggat tgtcgggagt
    15421 atcggcagcg ccattggcgg ggctgttggt ggcggcgcat ccgcgtcagg cggtacagcc
    15481 attcaggccg ctgcggcgaa attccatttt gcaaccggag gatttacggg aaccggcggc
    15541 aaatatgagc cagcggggat tgttcaccgt ggtgaatttg tcttcacgaa ggaggcaacc
    15601 agccggattg gcgtggggaa tctttaccgg ctgatgcgcg gctatgccac cggcggttat
    15661 gtcggtacac cgggcagcat ggcagacagc cggtcgcagg cgtccgggac gtttgagcag
    15721 aataaccatg tggtgattaa caacgacggc acgaacggtc agataggtcc ggctgctctg
    15781 aaggcggtgt atgacatggc ccgcaagggt gcccgtgatg aaattcagac acagatgcgt
    15841 gatggtggcc tgttctccgg aggtggacga tgaagacctt ccgctggaaa gtgaaacccg
    15901 gtatggatgt ggcttcggtc ccttctgtaa gaaaggtgcg ctttggtgat ggctattctc
    15961 agcgagcgcc tgccgggctg aatgccaacc tgaaaacgta cagcgtgacg ctttctgtcc
    16021 cccgtgagga ggccacggta ctggagtcgt ttctggaaga gcacgggggc tggaaatcct
    16081 ttctgtggac gccgccttat gagtggcggc agataaaggt gacctgcgca aaatggtcgt
    16141 cgcgggtcag tatgttgcgt gttgagttca gcgcagagtt tgaacaggtg gtgaactgat
    16201 gcaggatatc cggcaggaaa cactgaatga atgcacccgt gcggagcagt cggccagcgt
    16261 ggtgctctgg gaaatcgacc tgacagaggt cggtggagaa cgttattttt tctgtaatga
    16321 gcagaacgaa aaaggtgagc cggtcacctg gcaggggcga cagtatcagc cgtatcccat
    16381 tcaggggagc ggttttgaac tgaatggcaa aggcaccagt acgcgcccca cgctgacggt
    16441 ttctaacctg tacggtatgg tcaccgggat ggcggaagat atgcagagtc tggtcggcgg
    16501 aacggtggtc cggcgtaagg tttacgcccg ttttctggat gcggtgaact tcgtcaacgg
    16561 aaacagtgac gccgatccgg agcaggaggt gatcagccgc tggcgcatcg agcagtgcag
    16621 cgaactgagc gcggtcagtg cctcttttgt actgtccacg ccgacggaaa cggacggtgc
    16681 cgtttttccg ggacgtatca tgctggccaa cacctgcacc tggacctatc gcggtgatga
    16741 gtgcggttat cacggtccgg cggtcgcgga tgaatatgat cagccgacgt ccgatatcac
    16801 gaaggataaa tgcagcaaat gcctgagcgg ctgtaagttt cgcaataacg tcggcaactt
    16861 tggcggcttc ctttccatta acaaactttc gcagtgaatc ccatgacaga gacagaatca
    16921 gcgattctgg cgcacgcccg gcgatgtgcg ccagcggagt cgtgcggctt cgtggtgaga
    16981 acaccggagg gggaaagata ttttccctgc gtgaatatct ccggtgagcc ggaggcgtat
    17041 ttccggatgt cgccggagga ctggctgcgg gcagagatgc agggtgagat tgtggcgctg
    17101 gtccacagcc accccggtgg tctgccctgg ctgagtgaga ccgaccggcg gctgcaggtg
    17161 cagagtgatt tgccgtggtg gctggtttgc cggggggcga ttcacaagtt ccgctgtgtg
    17221 ccacatcttt ccgggcggcg ctttgagcac ggggtgacgg actgttacac gctgttccgg
    17281 gatgcttacc atctggcggg gattgagatg ccggattttc atcgcgagga tgactggtgg
    17341 cgtcacggtc agaatctcta tctggataat ctggaggcca cagggctgta tcaggtgccg
    17401 ttgtcatcag cacaaccggg cgatgtgctg ctgtgctgtt ttggttcatc ggtgccgaat
    17461 catgccgcca tttactgtgg tgacagcgag ctgctgcacc atattcctga acaactgagc
    17521 aaacgagaga ggtacaccga caaatggcag cgacgcacac actccctctg gcgtcaccgg
    17581 gaatggcacg catctgcctt tacggggatt tgcaacgatt tggccgccgc atcgaccttc
    17641 gtgtgaaaac gggggctgaa gccatccgcg cactggccac acagctcccg gcgtttcgtc
    17701 agaaactgag cgacggctgg tatcaggtac ggattgccgg gcgggacgtc agcacgtccg
    17761 ggttaacggc gcagttacat gagactctgc ctgacggcgc tgtgattcat attgttccca
    17821 gagtcgccgg ggccaagtca gggggcgtat tccagattgt tctgggggct gccgccattg
    17881 ccggatcatt ctttactgcc ggagccaccc ttgcagcatg gggggcagcc attggggccg
    17941 gtggtatgac cggcatcctg ttttctctcg gtgccagtat ggtgctcggt ggtgtggcgc
    18001 agatgctggc accgaaagcc agaactcccc gtacacagac aacggataac ggtaagcaga
    18061 acacctattt ctcctcactg gataacatgg ttgcccaggg caatgttctg cctgttctgt
    18121 acggggaaat gcgcgtgggg tctcgcgtgg tttctcagga gatcagcacg gcagacgaag
    18181 gggatggtgg tcaggttgtg gtgattggtc gctgatgaaa aacgtttatg tgaaaccgcc
    18241 tccgggcggt tttgtcgttt atggagcgtg aggaatgggt aaaggcagca gtaaggggca
    18301 tactccgcgc gaagcgaagg acaacctgaa gtccacgcag ttgctgagtg tgatcgatgc
    18361 catcagcgaa gggccgattg aaggtccggt ggatggatta aaaagcgtgc tgctgaacag
    18421 tacgccagtg ctggacagtg aggggaatac taacatctcc ggcgtcacgg tggtgttccg
    18481 ggccggtgag caggagcaga caccgccgga gggatttgaa tcctccggct ccgagacggt
    18541 gctgggtacg gaagtgaaat acgacacgcc gatcacctgg accatcacgt cggcaaacat
    18601 tgaccgactg cgtgttacct tcggcgtgca ggcactggtg gaaaccacct caaaggggga
    18661 caggaatccg tcggaagtcc gcctgctggt tcagatacag cgtaatggtg gctgggtgac
    18721 ggaaaaagac atcaccatta agggcaaaac cacctcgcag tatctggcct cggtggtggt
    18781 gggtaacctg ccgccgcgcc cgttcagtat acggatgcgc aggatgacgc cggacagcac
    18841 cacagaccag ctgcagaaca aaacgctctg gtcgtcatac accgaaatca tcgatgtgaa
    18901 acagtgctac ccgaacacgg cactggtcgg tgtgcaggtg gactcggagc agttcggcag
    18961 ccagcaggtg agccgtaatt atcatctgcg cgggcgtatt ctgcaggtgc cgtcgaacta
    19021 taacccgcag acgcggcaat acagcggtat ctgggacgga acgtttaaac cggcatacag
    19081 caacaacatg gcctggtgtc tgtgggatat gctgacccat ccgcgctacg gcatggggaa
    19141 acgtcttggt gcggcggatg tggataaatg ggcgctgtat gtcatcggcc agaattgcga
    19201 ccagtcggtg ccggacggct ttggcggcac ggagccgcgc atcacctgta atgcctggct
    19261 gaccacacag cgcaaggcgt gggatgtgct cagtgatttc tgctcggcga tgcgctgtat
    19321 gccgatatgg aacgggcaga cgctgacgtt cgtgcaggac cgaccatcag ataaggtgtg
    19381 gacctataac cgcagtaatg tggtgatgcc ggatgatggc gcgccgttcc gctacagctt
    19441 cagcgccctg aaggaccgcc ataatgccgt tgaggtgaac tggattgacc cggataacgg
    19501 ctgggagacg gcgacagagc ttgtggagga cacgcaggcc attgcccgtt acggtcgtaa
    19561 cgtcacgaag atggatgcct ttggctgtac cagtcggggg caggcgcacc gcgccgggct
    19621 gtggctgatt aaaacggaac tgctggaaac gcagaccgtg gacttcagcg tgggtgcgga
    19681 agggcttcgc catgtaccgg gggatgtcat tgaaatctgc gatgatgact atgcgggtat
    19741 cagcaccggc gggcgcgtgc tggcggtgaa cagccagacc cggacgctga cgctcgaccg
    19801 tgaaatcacg ctgccatcct ccggtaccac gctgataagc ctggttgacg gaagtggcaa
    19861 tccggtcagc gtggaggtcc agtccgtcac cgacggcgtg aaggtaaaag tgagccgtgt
    19921 tcctgacggc gttgccggat acagcgtgtg ggggctgaag ctgccgacgc tgcgccagcg
    19981 actgttccgc tgcgtgagta tccgtgagaa cgacgacggc acgtatgcca tcaccgccgt
    20041 gcagcatgtg ccggaaaaag aggccatcgt ggataacggg gcgcactttg acggcgacca
    20101 gagcggcacg gtgaatggtg tcacgccgcc agcggtgcag cacctgactg ccgaagtcac
    20161 cgcagacagc ggggaatatc aggtgctggc gcgctgggac acaccgaagg tggtgaaggg
    20221 cgtgagcttc ctgctccgtc tgaccgtaac agcggacgac ggcagtgagc ggctggtcag
    20281 cacggcccgg acgacggaaa ccacataccg cttcacacaa ctggcgttgg ggaactacag
    20341 gctgacagtc cgggcggtaa atgcgtgggg acagcagggc gatccggcat cggtatcgtt
    20401 ccggattgcc gcaccggcag cgccgtctcg gattgagctg acaccgggct attttcagat
    20461 aaccgccacg ccgcatcttg cggtttatga cccgacggta cagtttgagt tctggttctc
    20521 ggaaaaacgg attgcggata tcaggcaggt tgaaaccagc gcgcgttatc ttggtacggc
    20581 actgtactgg atagccgcca gtatcaatat caaaccgggc catgattatt atttttacgt
    20641 tcgcagtgtg aacaccgttg gcaaatcggc attcgtggag gctgtcggtc agccgagtga
    20701 tgatgcatca ggctatctgg attttttcaa aggcgagata gggaaaaccc atctggctca
    20761 ggagctgtgg acgcagattg ataacggtca gcttgcgcct gacctgactg aaatcaggac
    20821 gtccataacg gatgtcagca atgaaataac acagaccgtc aataagaaac tggaagacca
    20881 gagtgcagcg atccagcaga tacagaaggt tcaggttgat acaaataata acctgaacag
    20941 catgtgggca gtgaagctgc agcagatgca ggacggacgc ctttatattg cgggtatcgg
    21001 tgccggtatt gagaacaccc ctgacggcat gcagagtcag gtgctgctgg cagcagacag
    21061 gattgcgatg attaatcctg cgaatggcaa cacaaagccg atgtttgttg gtcagggcga
    21121 tcagatattc atgaatgaag tgttcctgaa acgcctgacg gcccccacca ttaccagcgg
    21181 cggtaatcct ccggcatttt ccctgacatc agacgggaga ctgacggcga aaaatgcgga
    21241 tatcagtggc agtgtgaatg cgaactcagg aacgctcaac aatgtcacga ttaaccagaa
    21301 ctgtacgatt aagggcatgc tggaggcgac ccaggtcaga ggagatttcg ttaaagctgt
    21361 atcaaaagcc ttcccgaaaa aagtcggtac gtggggtaac acggaaacac caaacggtac
    21421 ggttacagtc accatcagcg atgatcataa ctttgaccgc cagattatta ttccgcccat
    21481 tatttttaac ggtatagcgt atgacgatcc ggggagcgga aataacccag gaggcacgcg
    21541 atacacgggt tatggttttg aagttcgcaa aaacggcgta ttaatcgcat ccagagaaac
    21601 taaaggggcc attcccggta gttacagtgc agttattgat atgccgagtg gcaggggaag
    21661 cgtcactctg gagtttaaga ttttccagaa aggcaatcag ggggcaggca atatcaccga
    21721 ctgtacggtg attgtgacca aaaaagccgc ttccggcatc agtattcgtt gaaatattta
    21781 taaccccaat aaagggcgtc aggaatgacg ccttttttat tgcagaaaag cgagaggtaa
    21841 ttatgcgtaa agtttgtgca gcaattttgt ccgcagccat ttgtctggcc gtatccggtg
    21901 cgcctgcatg ggcgtctgaa catcagtcca cgctgagcgc ggggtatctt catgcctcga
    21961 cgaacgtccc cggcagcgat gatctgaacg ggattaacgt gaaataccgt tatgagttta
    22021 cggacacact ggggctggtg acgtcattca gctatgcagg agacaagaat cgccagctga
    22081 cccgttacag cgatacccgc tggcatgaag attccgttcg taaccgctgg ttcagcgtaa
    22141 tggcggggcc gtctgtgcgc gtgaatgaat ggttcagcgc gtatgcgatg gcgggtgtag
    22201 cttacagccg tgtgtcgact ttctccgggg attatcttcg cgtaactgac aacaagggga
    22261 aaaagcacga tgtgctgacc ggaagtgatg acggtcgcca cagcaacacg tctctggcgt
    22321 ggggggctgg cgtgcagttt aacccgaccg aatccgtggc cattgatatt gcttatgaag
    22381 gctccggcag tggtgactgg cgcactgacg gtttcatcgt gggtgtcggt tataaattct
    22441 gattagccag gtaacacagt gttatgacag cccgccggtt cagg
//
LOCUS       DAZGQL010000084        22247 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.084, whole genome shotgun sequence.
ACCESSION   DAZGQL010000084 DAZGQL010000000
VERSION     DAZGQL010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22247
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.084"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..573
                     /locus_tag="ACHKIR_003699"
     CDS             <1..573
                     /locus_tag="ACHKIR_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO9991379.1"
                     /translation="MLRLRRAGQITGQHVPEIILLNSHDGSSSYQMLPGYFRAICTNG
                     LVCGQSLGEVRVPHRGNVVDRVIEGAYEVVGVFDRIEEKRDAMQSLILPPPARQALAQ
                     AALTYRYGNEHQPVTTADILTPRRREDYGKDLWSAYQTIQENMLKGGISGRSAKGKRI
                     HTRAIHSIDTDIKLNRALWVMAETLLESMR"
     gene            912..1385
                     /locus_tag="ACHKIR_003700"
     CDS             912..1385
                     /locus_tag="ACHKIR_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO9991380.1"
                     /translation="MKTLSQNTTSSACAPETGLQQLVATIVPDEQRISFWPQHFSLIP
                     QWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNVMNGN
                     RAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            1401..1877
                     /locus_tag="ACHKIR_003701"
     CDS             1401..1877
                     /locus_tag="ACHKIR_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFO9991381.1"
                     /translation="MQQLSFLPGEMTPGERSLILRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGSQVFSFAEHGLL"
     gene            1946..2167
                     /locus_tag="ACHKIR_003702"
     CDS             1946..2167
                     /locus_tag="ACHKIR_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFO9991382.1"
                     /translation="MKIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            2330..2698
                     /locus_tag="ACHKIR_003703"
     CDS             2330..2698
                     /locus_tag="ACHKIR_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFO9991383.1"
                     /translation="MSDTHHETNYPDDYNNRPWWGLPCTITPCFGARLVQEGNRLYYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYARGLTCEAD
                     TLGSCGYVYMAVYPTPETKK"
     gene            2788..3165
                     /locus_tag="ACHKIR_003704"
     CDS             2788..3165
                     /locus_tag="ACHKIR_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFO9991384.1"
                     /translation="MNTLPDTHVREASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFS
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPRFSTSPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITRGKHPEAKR"
     gene            3162..3650
                     /locus_tag="ACHKIR_003705"
     CDS             3162..3650
                     /locus_tag="ACHKIR_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFO9991385.1"
                     /translation="MKLSLTLEADSINVRALNMGRIVVDVDGVELAELINVVCDNGHS
                     LRVVDESDRASTDCTPPFAALTGIRCSTAHITAKDSAWLYSLSHQTNDFGESEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPGLPTF
                     NW"
     gene            3670..3867
                     /locus_tag="ACHKIR_003706"
     CDS             3670..3867
                     /locus_tag="ACHKIR_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFO9991386.1"
                     /translation="MESLTTETALDILIAWLQNNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAREDIRTLRQLQFLQQNR"
     gene            3952..4818
                     /locus_tag="ACHKIR_003707"
     CDS             3952..4818
                     /locus_tag="ACHKIR_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFO9991387.1"
                     /translation="MSDITASRPEVVTGHTDVICSTSIRHILAVRKSTLLQIDTLIRQ
                     LAEISAMTESIGGKTALDWAMKQDFRCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMQSLMDAQTRDTWYRSLEYDNFPEISEANILSTFEQLHRSKDEVFERGVINVFKGLS
                     WDYKTNSPCKFGSKIIVNNLVRWDQWGFHLISGMQADRLADLERMLHLLSGKPIPDNR
                     GNITINLDDHIQSVQGKEDYEDEMFSIKYFKKGSVHITFRKPELVDRLNDIIARHYPE
                     ALASTVNKSRKA"
     gene            4890..5153
                     /locus_tag="ACHKIR_003708"
     CDS             4890..5153
                     /locus_tag="ACHKIR_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001143297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HFO9991388.1"
                     /translation="MPTSVSLSPYFETFIREQIESGRYNNTSEVIRAGLRALEEREQQ
                     IKLESLQSAVTAGINSGESKSAEEVFGRLTHKYKKMAEGEQPI"
     gene            5150..5476
                     /locus_tag="ACHKIR_003709"
     CDS             5150..5476
                     /locus_tag="ACHKIR_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO9991389.1"
                     /translation="MKLTVSPLAEHDLEAIGDWIAQDNPVRAISFTEELYQQCLLIAE
                     SPVLYRERPELGAGIRGCSYGRYLLLFRVLDTEVRIERIVHGSRDINRVLAEPDDKPS
                     DHFWQP"
     gene            complement(5890..6426)
                     /gene="yghD"
                     /locus_tag="ACHKIR_003710"
     CDS             complement(5890..6426)
                     /gene="yghD"
                     /locus_tag="ACHKIR_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspM family type II secretion system protein
                     YghD"
                     /protein_id="HFO9991390.1"
                     /translation="MLRDKFIHYFQQWRERQLSRGEHWLTQHLAGRSPREKGMLLAAV
                     VFLFSVGYYVLIWQPLSERIEQQETMLQQLVAMNTRLKNAAPDIIAARKSATTTPAQV
                     SRVISDSASAHSVVIKRIAERGENIQVWIEPVVFNDLLKWLNALDEKYALRVTQIDVS
                     AAEKTGMVNVQRLEFGRG"
     gene            complement(6428..7606)
                     /gene="gspL"
                     /locus_tag="ACHKIR_003711"
     CDS             complement(6428..7606)
                     /gene="gspL"
                     /locus_tag="ACHKIR_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HFO9991391.1"
                     /translation="MSSMLEIFIPLCASDPIRWQHRTTDVEHGIWSDVDNEQLQQWLQ
                     TDAIRLYIPGEWISVWQVELPDVARKQIPTILPALLEEELNQDIDELHFAPLKIDQQL
                     ATVAVIHQQHMRNIAQWLQANGITRATVAPDWMSIPCGFMAGDAQRVICRIDECRGWS
                     AGLALAPVMFRAQLNEQDLPLSLTVVGIAPEELSAWAGPDAERLTVTALPAITTYGEP
                     EGNLLTGPWQPRVSYRKQWARWRVMILPILLILVALAVERGVTLWSVSEQVAQSRTQA
                     EKQFLTLFPEQKRIVNLRSQVTMALKKYRPQADDTRLLAELSAIASTLKSASLTDIEM
                     RGFTFDQKRQTLHLQLRAANFASFDKLRSALAADYVVQQDALQKEGDAVSGGVTLRRK
                     "
     gene            complement(7603..8580)
                     /gene="gspK"
                     /locus_tag="ACHKIR_003712"
     CDS             complement(7603..8580)
                     /gene="gspK"
                     /locus_tag="ACHKIR_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HFO9991392.1"
                     /translation="MITSPPKRGMALVVVLVLLAVMMLVTITLSGRMQQQLGRTRSQQ
                     EYQQALWYSASAESLALSALSLSLKNEKRVHLAQPWASGPRFFPLPQGQIAVTLRDAQ
                     ACFNLNALAQPTTASRPLAVQQLIALITRLDVPAYRAELIAESLWEFIDEDRSVQTRL
                     GREDSEYLARSVPFYAANQPLADISEMRVVQGMDAGLYQKLKPLVCALPMTRQQININ
                     TLDVAQSVILEALFDPWLSPVQARALLQQRPAKGWEDVDQFLAQPLLADVDERTKKQL
                     KTVLSVDSNYFWLRSDITVNEIELTMNSLIVRMGPQHFSVLWHQTGESE"
     gene            complement(8577..9182)
                     /gene="gspJ"
                     /locus_tag="ACHKIR_003713"
     CDS             complement(8577..9182)
                     /gene="gspJ"
                     /locus_tag="ACHKIR_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HFO9991393.1"
                     /translation="MRRTCAGFTLLEMLVAIAIFASLALMAQQVTNGVTRVNSAVAGH
                     DQKLNLMQQTMSFLNHDLTQMMPRPVRGDQGQREPALLAGAGVLASESEGMRFVRGGV
                     VNPLMRLPRSNLLTVGYRIHGGYLERLAWPLTDAAGSVKPTTQKLIPADSLRLQFYDG
                     TRWQESWSSVQAIPVAVRMTLHSPQWGEIERIWLLRGPQLS"
     gene            complement(9179..9550)
                     /gene="gspI"
                     /locus_tag="ACHKIR_003714"
     CDS             complement(9179..9550)
                     /gene="gspI"
                     /locus_tag="ACHKIR_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HFO9991394.1"
                     /translation="MKRGFTLLEVMLALAIFALAAMAVLQIASGALSNQQILEEKTVA
                     GWVAENQTALLYLMTREQRAVRHQGESDMAGSRWYWRTTPLSTGNALLQAVDIEVSRH
                     EDFSSVIQSRRAWFSAVGGQQ"
     gene            complement(9547..10110)
                     /gene="gspH"
                     /locus_tag="ACHKIR_003715"
     CDS             complement(9547..10110)
                     /gene="gspH"
                     /locus_tag="ACHKIR_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001394829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HFO9991395.1"
                     /translation="MPERGFTLLEIMLVIFLIGLASAGVVQTFATASEPPAKKAAQAF
                     LTRFAQFKDRAVIEGKTLGVLIDPPGYQFMQRRHGQWLPVSATRLSAQVTVPKQVQML
                     LQPGSDIWQKEYALELQRRRLTLHDIELELQKEAKKKTPQIRFSPFEPATPFTLRFYA
                     SAQNACWAVKLAHDGVLSLNQCDEGMP"
     gene            complement(10114..10569)
                     /gene="gspG"
                     /locus_tag="ACHKIR_003716"
     CDS             complement(10114..10569)
                     /gene="gspG"
                     /locus_tag="ACHKIR_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003912123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HFO9991396.1"
                     /translation="MNSLSRTQKPRAGFTLLEVMVVIVILGVLASLVVPNLLGNKEKA
                     DRQKAISDIVALENALDMYRLDNGRYPTTEQGLEALIQQPANMADSRNYRTGGYIKRL
                     PKDPWGNDYQYLSPGEKGLFDVYTLGADGQENGEGAGADIGNWNLQEFQ"
     gene            complement(10586..11809)
                     /gene="gspF"
                     /locus_tag="ACHKIR_003717"
     CDS             complement(10586..11809)
                     /gene="gspF"
                     /locus_tag="ACHKIR_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HFO9991397.1"
                     /translation="MALFYYQALERNGRKTKGMIEADSARHARQLLRGKELIPVHIEA
                     RLNASTGGMLQRRRHAHRRVAAADLALFTRQLATLVQAAMPLETCLQAVSEQSEKLHV
                     KSLGMALRSRIQEGYTLSDSLREHPRVFDSLFCSMVAAGEKSGHLDVVLNRLADYTEQ
                     RQRLKSRLLQAMLYPLVLLVVATGVVTILLTAVVPKIIEQFDHLGHALPASTRTLIAM
                     SDTLQTSGVYWLAGLLGLLVLGQRLLKNPAMRLRWDKTLLRLPVTGRVARGLNTARFS
                     RTLSILTASSVPLLEGIQTAAAVSANRYVEQQLLLAADRVREGSSLRAALAELRLFPP
                     MMLYMIASGEQSGELETMLEQAAVNQEREFDTQVGLALGLFEPALVVMMAGVVLFIVI
                     AILEPMLQLNNMVGM"
     gene            complement(11809..13302)
                     /gene="gspE"
                     /locus_tag="ACHKIR_003718"
     CDS             complement(11809..13302)
                     /gene="gspE"
                     /locus_tag="ACHKIR_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HFO9991398.1"
                     /translation="MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLS
                     VLQELQRGLNAPFTLRQIDEAEFEQRLNAVWQRDSSEARQLMEDLGSAEDFFTLAEEL
                     PETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
                     PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVL
                     RLLDKNQARLTLERLGLSQQLTVQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELN
                     NHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQDPDVVMVGEIRDTET
                     AEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCP
                     DCRQPAPATDEEKRLLGITDARTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRD
                     LIHRQAGELELERYVRQHSAGIRSNGIEKVLAGETSLDEVLRVTMEA"
     gene            complement(13302..15362)
                     /gene="gspD"
                     /locus_tag="ACHKIR_003719"
     CDS             complement(13302..15362)
                     /gene="gspD"
                     /locus_tag="ACHKIR_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HFO9991399.1"
                     /translation="MFWRDMTLSIWRKKTTGLKTKKRLLPLMLAAALCSSPVWAEEAT
                     FTANFKDTDLKSFIETVGANLNKTIIMGPGVQGKVSIRTMTPLNERQYYQLFLNLLEA
                     QGYAVVPMENDVLKVVKSSAAKVEPLPLVGEGSDNYAGDEMVTKVVPVRNVSVRELAP
                     ILRQMIDSAGSGNVVNYDPSNVIMLTGRASVVERLTEVIQRVDHAGNRTEEVIPLDNA
                     SASEIARVLESLTKNSGENQPATLKSQIVADERTNSVIVSGDPATRDKMRRLIRRLDS
                     EMERSGNSQVFYLKYSKAEDLVDVLKQVSGTLTAAKEEAEGTVGSGREVVSIAASKHS
                     NALIVTAPQDIMQSLQSVIEQLDIRRAQVHVEALIVEVAEGSNINFGVQWASKDAGLM
                     QFANGTQIPIGTLGAAISQAKPQKGSTVISENGATTINPDTNGDLSTLAQLLSGFSGT
                     AVGVVKGDWMALVQAVKNDSSSNVLSTPSITTLDNQEAFFMVGQDVPVLTGSTVGSNN
                     SNPFNTVERKKVGIMLKVTPQINEGNAVQMVIEQEVSKVEGQTSLDVVFGERKLKTTV
                     LANDGELIVLGGLMDDQAGESVAKVPLLGDIPLIGNLFKSTADKKEKRNLMVFIRPTI
                     LRDGMAADGVSQRKYNYMRAEQIYRDDQGLSLMPHTAQPVLPAQNQALPPEVRAFLNA
                     GRTR"
     gene            complement(15392..16351)
                     /gene="gspC"
                     /locus_tag="ACHKIR_003720"
     CDS             complement(15392..16351)
                     /gene="gspC"
                     /locus_tag="ACHKIR_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HFO9991400.1"
                     /translation="MARVVFRDARIYLIQWLTKIRHILSQRQSLNTDKEHLRKMVRGM
                     FWLMLLIISAKMAYSLWRYFSFSAEYTVVSSSANKPLRADAKPFDKNDVQLVSQQNWF
                     GKYQPVAAPVKQPESAPVAETRLNVVLRGIAFGARPGAVIEEGGKQQVYLQGERLGSH
                     NAVIEEINRDHVMLRYQGKMERLSLAEEERPPVAVTSKKAVSDEAKQAVAEPAASAPV
                     EIPAAVRQALAKDPQKIFNYIQLTPVRKEGIVGYAVKPGADRSLFDASGFREGDIAIA
                     LNQQDFTDPRAMIALMRQLPSMDSIQLTVLRKGARYDISIALR"
     gene            complement(16369..16779)
                     /gene="gspS2"
                     /locus_tag="ACHKIR_003721"
     CDS             complement(16369..16779)
                     /gene="gspS2"
                     /locus_tag="ACHKIR_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system pilot lipoprotein
                     GspS-beta"
                     /protein_id="HFO9991401.1"
                     /translation="MSIKQMPGRVLISLLLSVTGLLSGCASHNENASLLAKKQAQNIS
                     QNLPIKSAGYTLVLAQSSGTTVKMTIISESGTQTTQTPDAFLTSYQRQMCADPTVKLM
                     ITEGINYSITINDTRTGNQYQRKLDRTTCGIVKA"
     gene            complement(16845..17654)
                     /gene="pppA"
                     /locus_tag="ACHKIR_003722"
     CDS             complement(16845..17654)
                     /gene="pppA"
                     /locus_tag="ACHKIR_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase PppA"
                     /protein_id="HFO9991402.1"
                     /translation="MFFDVFQQYPAAMPVLATVGGLIIGSFLNVVIWRYPIMLRQQMA
                     EFHGEMPSTQSKISLALPRSHCPHCQQTIRVRDNIPLLSWLMLKGRCRDCQAKISKRY
                     PLVELLTALAFLLASLVWPESGWALAVMILSAWLIAASVIDLDNQWLPDVFTQGVLWT
                     GLIAAWAQQSPLTLQDAVTGVLVGFITFYSLRWIAGIVLRKEALGMGDVLLFAALGGW
                     VGALSLPNVALIASCCGLIYAVITKRGSTTLPFGPCLSLGGIATLYLKALF"
     gene            complement(17784..22247)
                     /gene="sslE"
                     /locus_tag="ACHKIR_003723"
                     /pseudo
     CDS             complement(17784..22247)
                     /gene="sslE"
                     /locus_tag="ACHKIR_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026189.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="lipoprotein metalloprotease SslE"
BASE COUNT         5048 a         6275 c         5642 g         5282 t
ORIGIN      
        1 atgctgcgtc tgcggcgggc cggacagata accggtcagc atgtgcctga aattattctg
       61 ctcaactccc atgacggttc atccagctac cagatgttac ccggatattt tcgtgccatc
      121 tgcaccaatg gcctggtctg cggtcagtcg ctgggagaag tccgggtgcc acaccgggga
      181 aacgtggtgg acagggtgat tgaaggggct tacgaggtgg tgggcgtttt tgaccggata
      241 gaggagaagc gtgatgccat gcagtcgctg attctgccgc caccggcacg ccaggcgctg
      301 gcacaggcgg cactgactta ccgttatggt aatgagcatc agcccgtcac caccgctgat
      361 attctgacac cgcgacgccg ggaggattac ggtaaggacc tgtggagtgc ttatcagacc
      421 atacaggaga atatgctgaa aggcgggatt tccggccgca gtgcaaaagg aaaacgtatc
      481 cacacccgtg ccattcacag catcgacacc gatattaagc tcaaccgcgc attgtgggta
      541 atggcagaaa cgctgctgga gagcatgcgc tgataaaaca tatccctgaa ggcgtgattc
      601 aggtaccaga atcacccgac gccccggagg cgctgccggg gtgtcgttcc atttctgatg
      661 atgacctgtg cacctggtgt acccgtctgc tttaccgtcc cggagaactc agtctgtgca
      721 gactgagtac aggcaatggc atctggccat ccgtctgtga ccggaacggt tacgcgtacg
      781 gctgtccgga atttcagccg aatatcattc atccctgata ttgccgggtc tcacggtcag
      841 tgatgaacgt gatgttgtca gcccccggcc tttatttcct ctcatttatt cacattacaa
      901 ggatttcaca catgaaaaca ttgtctcaga acaccacctc ttcagcctgt gcaccggaga
      961 ctggcctgca acaactggtt gccaccatag tccctgatga acagcgcatc agcttctggc
     1021 cgcagcattt tagcctcatt ccacagtggg tgaccctgga gccccgtgtc ttcggctgga
     1081 tggaccgttt gtgcgaagac tactgcggtg gtatctggaa tctgtacacc ctgaacaacg
     1141 gcggcgcatt tatggcccct gaaccggatg acgatgatga cgaaacatgg gtgctgttca
     1201 atgtcatgaa cggtaaccgc gctgaaatga gtccggaagc cgccggcatt gcggcctgtc
     1261 tgatgacgta cagtcatcac gcctgtcgta cggagtgtta tgccatgacg gtccactatt
     1321 accggctgcg ggattacgcc ctgcagcatc cggaatgcag cgccattatg cgcatcattg
     1381 actgaatgga ggcagggaca atgcagcagc tttcctttct gcccggagag atgacgcccg
     1441 gcgagcgcag tctcattctg cgggccctga aaaccctgga ccgccatctt catgaacccg
     1501 gcgtggcctt cacctccacc cgtgcggcac gggaatggct gattctgaac atggcaggac
     1561 tggagcgtga agagttccgg gtgctgtatc tgaacaacca gaatcagttg attgccggtg
     1621 aaaccctctt cacaggcacc atcaaccgca cagaagtcca tccccgggag gtgattaaac
     1681 gcgccctgta ccacaatgcc gccgccgtgg tgctggcgca caatcacccg tccggtgaag
     1741 tcacccccag taaggcagac cgccttatca ccgaacgtct ggtacaggca ctgggcctgg
     1801 tggatatccg ggtgccggac catctgatag tcggtggcag ccaggttttc tcctttgccg
     1861 aacatggtct gctttaaccc gtcaccgcca caatcacatc acacctgttt tcacttttat
     1921 cttctgtctt cagaggtatc ccattatgaa aattatcacc cgtggtgaag ccatgcgtat
     1981 tcaccaacag catcctgcat cccgtctatt tccgttctgc accggtaagt accgctggca
     2041 cggcagtgct gaggcgtata ccggtcgtga agtgcaggat attcccggtg tgctggccgt
     2101 gtttgctgaa cgccgtaagg acagttttgg cccgtatgtc cggctgatga gcgtcactct
     2161 gaactaatgt tccgtcaccc ggaaaacgag tacgaccaga tgacctcaga cgccgctgtg
     2221 gatgaggccc ttctcatcaa tgaatacatc ttcaccgaag ccggtctcca caccggctga
     2281 cctgtcatcg ttaacccctt cttaaccgat tttctgtgag gactttatcg tgtcagacac
     2341 gcaccatgag acaaattatc ccgacgacta taacaaccgc ccctggtggg ggctgccctg
     2401 caccataaca ccctgttttg gcgcccgtct ggtgcaggag ggtaaccggt tgtattacct
     2461 tgccgaccgc gccggtatca gaggccggtt cagcgacgcg gatgcgtacc atctggacca
     2521 ggcctttccg ctgctgatga aacaactgga actcatgctc accagcggtg aactgaatcc
     2581 ccgccatcag cataccgtca cgctgtatgc cagaggactg acctgcgaag ccgacaccct
     2641 cggctcctgt ggttacgttt atatggctgt ttatccgacg cccgaaacga aaaagtaaat
     2701 catcagaata accttctgct ccggcctggt gttttcacca cgccactttc ccatttttca
     2761 tctctgcata tcaggaaaat cttcagtatg aacacattac ccgacacaca cgtacgggag
     2821 gcatcgcgct gcccatctcc agtaaccatc tggcagacac tgctcacccg actgctggac
     2881 cagcactacg gcctcacgct aaatgacaca ccgttctccg atgaacgtgt gattgagcag
     2941 catattgagg caggcatttc actgtgtgac gcggtgaact ttctcgttga aaaatacgcg
     3001 ctggtgcgta ccgaccagcc ccggttcagc actagtcccc gctctcagtt aataaacagt
     3061 attgatatcc tccgggctcg ccgggcgacc ggcctgatga cccgcgacaa ttacagaacg
     3121 gtaaataaca ttacccgggg taagcatccg gaggcgaaac gatgaaactt tccctgacgc
     3181 tggaagccga cagcattaac gtgcgggcgc tgaacatggg gcgcattgtc gttgacgtcg
     3241 atggtgttga gcttgctgaa ctgattaacg tggtctgcga taacggtcac tcccttcgtg
     3301 ttgttgatga atctgaccgg gcctcaacag actgcacgcc accatttgct gccctgaccg
     3361 gcatacgctg cagtaccgcg catatcacgg caaaagacag cgcctggctg tactcgctgt
     3421 cacatcagac gaatgacttc ggcgaatcag aatggattca ttttaccggg tccggttatc
     3481 tgttacgtac tgatgcgtgg tcgtacccgg ttctgcgact taaacgcctg ggcctgtcaa
     3541 aaacgtttcg tcgtctggtc gtaacgctca tccgccgtta tggcgtcagt ctcattcatc
     3601 tggatgccag cgctgaatgt ctgccgggtt tacccacctt caactggtaa ccgggacaaa
     3661 agacacagta tggaatcatt aaccacagaa acggcgctgg atattctgat tgcgtggcta
     3721 cagaacaata tcgactgcga atccggaatt atcttcgaca acgatgagga taaaacggat
     3781 tcggcagcac tgctgccctg cattgaacag gccagggaag atatccgtac actgcgccaa
     3841 ctgcagtttc tgcaacagaa ccggtgagtc ttactcacca gacttcattc cactgacgcc
     3901 agcctgaata cggctggcgt tttcgtttat aaaccaaaag gaaaaacgat gatgtcagat
     3961 atcacagcct cccgtccgga agtggtcacc gggcatacgg acgttatttg ctcaaccagt
     4021 atccgccaca ttctggctgt acgaaagagt acactgctgc aaatcgacac acttatccgg
     4081 caactggctg aaatctcagc aatgacagaa agtattggcg gtaaaaccgc actggactgg
     4141 gcgatgaaac aggattttcg ctgcggctgc tggctgatgg agaaaccgga aaccgcaatg
     4201 aaagccatca cacgcaatct cgatcgcgaa atctggcgtg acctgatgca acgttccggg
     4261 atgcagagtc tgatggatgc gcagacccgg gacacatggt acaggtcact ggaatatgat
     4321 aattttccgg aaatcagtga agcgaacatt ctgagcacat ttgaacaact gcaccggagt
     4381 aaggatgaag tgtttgagcg gggagtgatc aacgtcttca aagggctgag ctgggattac
     4441 aaaaccaact caccctgtaa atttggcagt aaaattatcg tcaacaatct ggtgaggtgg
     4501 gaccagtggg gatttcatct tatcagtgga atgcaggcag atcgcctggc tgacctggaa
     4561 agaatgttgc atctgctcag cggtaaaccg atccccgaca accgggggaa tatcaccatt
     4621 aatctggatg accacataca gtctgttcag ggtaaagagg actatgaaga cgagatgttc
     4681 agtattaaat actttaagaa aggctcagta cacatcacgt tcaggaaacc agaactggtt
     4741 gacaggctta atgatattat tgccagacac tacccggaag ccctggcaag cacagtgaac
     4801 aaaagccgca aggcataaaa gaggtatttt acaattcgcg aatattaact attattaatt
     4861 gatagcaatc aatcggcgag aaaatcacca tgcctaccag tgtatccctc agtccttact
     4921 ttgaaacgtt tatccgcgaa caaatagaaa gtggtcggta taacaataca agtgaagtca
     4981 ttcgtgccgg acttcgtgct ctggaagaac gggaacagca gataaagctt gaatcactcc
     5041 agagcgccgt taccgcaggc atcaacagcg gtgaaagtaa aagtgctgaa gaggtattcg
     5101 ggcgactgac gcacaaatac aaaaagatgg cagaaggtga acagccaata tgaaactgac
     5161 tgtttcacca ttagcagaac acgatcttga agctataggt gactggatcg ctcaggataa
     5221 tcctgtccgt gcaatcagtt ttaccgaaga gctttaccaa caatgtctgt tgatagccga
     5281 atcgccagta ctctacagag aaagacccga attaggggct gggatcagag gttgttcgta
     5341 tggtcgttac cttctgctgt tcagggtact ggatactgaa gttcgtatcg aacgtatcgt
     5401 tcatggttcc cgggatatca acagggtact tgcagagcca gacgacaaac catcagatca
     5461 tttctggcag ccataaacaa aatctgtgcg ttaacgctcc ctaacacagg tagccaggta
     5521 ttttataaat ctctgtattt tgttgttact tcgattccgc aattcagcat atcgaaccag
     5581 acaaaatgat tacagcaaag cacaaaaaag agggattata gcgtcaaaag aattcgaatt
     5641 gcacatgggg gcctcattgg tggcctcaaa aacaacaaag acaaaacaat cgatacaaaa
     5701 caattggtta tataaccagt ttcattccga gtccgggcac cactattcta aagaacccgc
     5761 tcacaaggcg ggtttttgct tttggatctg acaataacct tcacgaaaca aattagctta
     5821 taaagcctgg gggaattact ctcgccacgt taacgagagt aattttattg atattaatct
     5881 cctgatactt tatccccgtc caaactccag ccgctgcaca ttcaccatcc cagtcttctc
     5941 agcagcactg acatcaattt gtgtcacccg cagcgcatat ttttcatcca gtgcgtttaa
     6001 ccatttcagc aggtcattaa acaccacagg ttctatccag acctgaatat tctccccacg
     6061 ctcggctatc cgcttgatga ccaccgagtg cgcggaagca ctgtcactga tgacccgcga
     6121 tacctgcgca ggcgttgttg tggcagattt tcgcgctgca ataatatccg gcgcggcgtt
     6181 cttcagtcgg gtgttcattg ccaccagctg ctgcaacatc gtctcctgtt gttctatccg
     6241 ttcgctcaac ggctgccaga tgaggacgta atatccgacg ctaaacagga acaccaccgc
     6301 tgccagtaac atgccctttt cacgcggaga acgccccgcc agatgttgtg tcagccagtg
     6361 ttcgccacgg cttaactggc gttcacgcca ttgctgaaaa tagtgaataa atttatcgcg
     6421 taacatgtta tttcctccgc aacgttacgc cgccggaaac cgcatcaccc tctttctgta
     6481 acgcgtcctg ttgcacaaca taatctgccg ccagtgcgct acgcagtttg tcgaagctgg
     6541 caaagttcgc agcccgtagc tggaggtgaa gcgtctggcg tttttgatca aaggtgaaac
     6601 cacgcatttc gatgtcggta agtgacgctg atttcagggt actggcgatc gctgacaatt
     6661 ctgcgagcag ccgggtatcg tcggcctgcg ggcgatattt tttcagcgcc atcgtcacct
     6721 gagagcgtaa attcacaatc cgcttctgct ccgggaacag tgttaagaac tgtttctccg
     6781 cctgggtgcg gctttgcgcc acctgttcgc tgacgctcca taacgtcacg ccccgttcca
     6841 ctgccagcgc aaccagaatc agcaatatcg gcagaatcat cacccgccag cgcgcccact
     6901 gttttcggta gctgacgcgg ggctgccacg gccctgttag caggttccct tccggttcgc
     6961 cataagtggt aatggcgggc agagccgtaa cggtcaggcg ttcagcatct gggccagccc
     7021 atgctgatag ctcttccggt gcaatgccga ccacggttag tgaaagcggt aaatcctgct
     7081 cattgagctg tgcgcggaac atgaccggag ccagcgccag cccggcgctc catccccggc
     7141 attcatcgat gcggcagata acccgttgcg catcgccagc cataaaccca caaggaatgg
     7201 acatccagtc tggcgcgacg gtagcgcggg tgatgccgtt tgcctgcaac cactgcgcaa
     7261 tgttgcgcat atgctgttgg tgaatcacag ctacggttgc cagttgctgg tcgattttca
     7321 acggggcgaa atgcagttca tcgatatcct ggttcagctc ttcttccagt aaggcgggca
     7381 gaatagtcgg tatctgcttg cgggcgacat caggcagttc aacctgccag acgctgatcc
     7441 attcgccggg aatgtagagt cgaatcgcat cagtttgcag ccattgctgg agctgttcgt
     7501 tgtcaacgtc agaccagatg ccgtgctcca cgtcggtggt acggtgctgc caacggatgg
     7561 gatcggaggc gcaaagcggg ataaaaatct caagcatgga actcactcac tttctcctgt
     7621 ctgatgccag agaaccgaaa agtgttgtgg gcccatgcgg acaattaacg agttcatcgt
     7681 cagttcaatc tcattcacgg tgatatctga acgcagccag aagtaattgc tgtccacgct
     7741 caggacggtt tttagctgtt ttttagtacg ctcatcgacg tcagcaagta gcggctgtgc
     7801 aagaaactga tcgacatctt cccagccctt cgccggacgt tgttgtaata acgcccgcgc
     7861 ctgaacaggg cttaaccacg ggtcaaacag cgcctcaaga atcacacttt gcgcgacgtc
     7921 cagggtatta atgttgattt gctggcgggt catcggcagc gcacagacca gcggtttcag
     7981 tttttgataa agtccggcgt ccattccctg caccacgcgc atctcgctga tatcagccag
     8041 cggttgatta gcggcgtaaa acggcaccga acgggcgaga tactcgctgt cttcacggcc
     8101 cagacgcgtc tgcacgctgc ggtcttcgtc aataaactcc cacaggcttt cggctatcag
     8161 ttcggcccga taagcaggca catccaggcg cgtgatcagg gcaatcagtt gttgtaccgc
     8221 gagcggacgc gacgccgtcg tcggctgagc gagagcattc aggttaaagc aggcctgtgc
     8281 gtcacgcaga gtgacggcga tttgcccctg cggcagtggg aaaaaacgag ggccggaagc
     8341 ccacggctgc gccagatgca cgcgcttttc atttttcagg ctcagactga gtgcgctcaa
     8401 cgccaggctt tccgcactgg cgctgtacca cagcgcctgc tggtactcct gctggctgcg
     8461 cgttcgccca agttgttgct gcattcgccc ggaaagcgtg atggtcacca gcatcataac
     8521 cgccagcaat accagcacca cgaccagtgc cattccgcgt tttggtggtg aggtgatcat
     8581 gataattgcg gcccgcgtaa caaccagatg cgttcaatct cgccccattg cggcgaatgc
     8641 agagtcatgc gcaccgccac tggtatcgcc tgcactgatg accagctctc ctgccagcgc
     8701 gtgccgtcgt agaactgcaa acggagcgaa tccgccggga ttaatttttg cgttgttggc
     8761 ttcacgctgc ctgccgcatc ggtcagtggc caggctaacc gttcgagata accaccatga
     8821 atgcggtaac cgacggtgag cagattactg cgcggcagac gcatcaacgg attaaccacg
     8881 ccgccgcgca caaaacgcat cccttcactc tcagacgcca gcacgccagc gcccgccagt
     8941 aacgctggtt cacgctggcc ctgatcgcct cttaccggac gcggcatcat ttgtgtcaga
     9001 tcgtggttca aaaaactcat cgtttgctgc atgaggttta gtttttgatc gtgtccggcg
     9061 acggcgctat tcacgcgtgt aacgccgttt gtcacctgct gcgccatcag tgccagtgag
     9121 gcaaaaatgg caattgccac cagcatttcc agtaacgtga aaccagcgca agtccttctc
     9181 actgttggcc tcccacggcg ctaaaccagg cgcgtcgtga ctgaatcacc gacgaaaagt
     9241 cttcgtgacg gctgacttca atatccactg cctggagcag cgcgttgcct gtactcagcg
     9301 gtgtggttcg ccagtaccag cggcttcctg ccatatcact ctcgccctgg tgcctgaccg
     9361 ctcgttgttc acgggtcatc aggtaaagca gtgcggtctg gttttcagct acccagcccg
     9421 ctaccgtttt ttcttcaagg atttgctgat tactcagcgc gccgctggcg atctgtaaca
     9481 ccgccatggc ggccaacgca aaaatcgcca gcgcgagcat tacttccagc aaggtaaatc
     9541 cacgcttcat ggcatcccct catcacattg attgagggat aacacgccat cgtgcgccag
     9601 ctttaccgcc caacacgcgt tttgtgccga cgcgtaaaaa cgcagtgtaa acggcgtggc
     9661 gggttcaaaa ggcgaaaaac ggatctgcgg tgtcttcttt ttcgcctctt tttgcagctc
     9721 cagttcaata tcgtgcagcg tcaggcgacg acgctgcagc tccagcgcat attccttctg
     9781 ccagatatca cttccgggtt gtaacagcat ctgcacctgt ttcggcaccg taacctgtgc
     9841 cgataaccgg gtcgcagaaa cgggcaacca ctgcccgtga cgacgctgca taaactggta
     9901 acctggtggg tcgattagca caccgagtgt tttcccttcg atcactgccc tgtccttaaa
     9961 ctgcgcaaag cgagtcagaa aagcctgcgc cgcttttttc gcaggcggct ctgaagcggt
    10021 cgcaaacgtc tgtaccacgc ccgcactggc aaggccgata aggaaaatca ccagcatgat
    10081 ttccagaagt gtgaatccgc gttcaggcac tgattactga aactcctgca aattccagtt
    10141 accgatatct gcgccagcgc cctccccgtt ttcctgacca tctgccccca gggtatacac
    10201 atcaaacagt cctttttcac ccgggctgag atactgataa tcattgcccc acggatcttt
    10261 tggcagtcgt ttaatgtatc caccggtacg gtagttgcgg gagtccgcca tattggccgg
    10321 ttgctggatc agcgcctcca gtccctgttc ggtagtcgga taacgcccgt tatccagtcg
    10381 gtacatatcc agcgcattct ccagcgccac gatatcgctg atggcttttt gccgatcggc
    10441 tttctctttg ttgcccagca ggttaggcac caccagactt gccaggacgc caagaataac
    10501 aatcaccacc atcacttcca gcagggtaaa acctgcccgt ggtttttgtg tgcgggataa
    10561 cgaattcatg tgataactcc gtaaattaca ttccaaccat attgttcagt tgcagcatcg
    10621 gttcgaggat ggcgatgacg ataaacagca ccacgcccgc catcatcacc accagcgccg
    10681 gctcaaacag ccctaacgcc agccccacct gggtatcaaa ttctcgttcc tggttaactg
    10741 cggcctgttc aagcatggtt tccagttcac cgctctgttc gccggaggcg atcatgtaca
    10801 gcatcatcgg tgggaacagg cgcaactccg ccagcgcagc gcgcagactg cttccttcgc
    10861 ggacgcgatc tgccgccagc agcagttgtt gttcgacata gcgatttgcc gacacggcgg
    10921 cagcggtctg aatgccttcc agtagcggaa cactgctggc ggtgaggatg cttaacgtgc
    10981 gggaaaaacg cgccgtattc agcccgcgcg caacacgccc cgtcacgggc aaacgcagca
    11041 aggttttatc ccagcgtagg cgcatggcag gatttttgag tagccgttgc cccagcacca
    11101 gaagccccag caatcccgcc agccagtaaa cgccgctggt ctgtaacgtg tcgctcatgg
    11161 cgatgagcgt gcgggtggag gcaggcagcg cgtgtccgag atgatcaaac tgttcgatga
    11221 ttttcggcac cactgccgtc agcaaaatag tgactacgcc cgttgccacc accagcaata
    11281 ccagcggata gagcatggcc tgcaacagtc gtgatttcag gcgctgtcgc tgttcggtgt
    11341 aatccgcaag gcgattgagc accacgtcga gatgcccgga tttttctccg gcagcgacca
    11401 tcgaacaaaa cagggaatca aagacgcggg gatgttcgcg caggctgtcc gacagggtat
    11461 aaccttcctg aatccggctg cgcagcgcca ttccgaggct tttcacatgc agtttttcac
    11521 tttgctcact gaccgcctgt aagcaggttt ccagcggcat tgctgcctgc accagcgttg
    11581 ccagttggcg cgtgaacagc gccaaatctg ccgccgccac gcgacgatgt gcgtgccgcc
    11641 gacgctgcaa cattccccct gtcgaagcat tcagccgggc ttcaatgtgc acggggataa
    11701 gctctttacc acgcaacaac tggcgggcat gacgcgcgga atccgcctca atcattcctt
    11761 tggttttgcg accattacgc tccagcgcct gatagtaaaa cagtgccatt acgcctccat
    11821 agttacccgc agaacttcat cgagagaggt ttctccggcg agcactttct caatgccgtt
    11881 gctgcggatg cccgcagagt gttgccggac ataacgttcc agctccagtt ccccggcctg
    11941 acggtggatc aaatcacgca atgtggcgtc caccacgatc aactcatgga tggcagtacg
    12001 cccgcggaaa cctttgtgat tacaggcggg gcagccctgc ggatggtaca gcgtgacagt
    12061 acgggcgtcg gtaatcccca gcaggcgttt ttcttcgtca gtggcaggcg cgggctggcg
    12121 gcagtcgggg cacagcgtgc ggaccagtcg ctgcgccatc acgcccgtca ggctggaaga
    12181 gagcaggaaa ggctcaacgc ccatatcctg cagacgtgtg atcgccccca ccgccgtgtt
    12241 ggtatgcagc gtggaaagta ccaggtgccc cgtgagcgac gcctgaacag cgatttctgc
    12301 ggtttctgta tcgcggattt caccgaccat caccacatcc gggtcctgac gcaaaatcgc
    12361 gcgcaggcca cgggcaaagg tcatgccgac gcgggtgttg acctgcgtct gaccgatccc
    12421 ttcaatcatg tattcgatgg ggtcttcaac cgtgagaatg ttgcgcgaat ggttgttcag
    12481 ctcctgcaat ccggcgtaca gcgtggtgct tttgccggaa cccgtcggtc ccgtcaccag
    12541 aaagatgccg tgcggtttgt gtaacagctg gcgcaactgc acggtcagtt gctgactcaa
    12601 ccccagacgt tccagcgtca ggcgggcctg gtttttgtcc agcagtcgca gcaccacgcg
    12661 ctcgccccag gcggagggca tggtggagac acgcacgtca atcgcccgac cgcccagcag
    12721 cagcgcaata cgcccatcct gcggtacgcg cttttcggca atatccagcc gcgccatcac
    12781 cttgatacgc gataccagca gcgaggccag tttgcgcccc gggcgcagca tttcatgtaa
    12841 tgtgccgtca acacgaaaac ggatcaccag actcttttca aatgtctcga tgtggatatc
    12901 cgaagcgcct tctttaatcg cctctgccag catggcgttg atcagtttga tgattggcgc
    12961 gtcgtcgtcg ctttccagca gatcttccgt ttccggcagt tcttcggcga gggtaaaaaa
    13021 atcctcagca gaaccgagat cttccatcag ctggcgggcc tcggaagagt cccgctgcca
    13081 gaccgcattc agccgctgtt caaattcggc ttcgtcgatt tgccgcagcg taaagggcgc
    13141 gttcagcccc cgctgcagct cctgcaaaac agagagcgac aacggatgaa cgtggaggat
    13201 ctccagcgac gcttcgcacc atgccaccag gctaaaccga cggctgaaac tgtagggcag
    13261 acgcacggtg ttagcggtgg tttcctgtgc tacaggcacc attaacgcgt tctcccggca
    13321 ttgaggaatg cgcgaacttc cggcggtaag gcctggtttt gcgctggcag taccggctgc
    13381 gcggtgtgcg gcatcaggct taagccttga tcatcgcgat agatctgctc ggcgcgcatg
    13441 tagttatatt tgcgctgcga cacgccgtct gccgccatac cgtcacgcag aatggtcggg
    13501 cggataaaca ccatcaggtt acgtttttct tttttatccg ccgtcgattt aaacaggtta
    13561 ccaatcaacg ggatatcgcc cagcagcggc actttcgcca cgctttctcc cgcctggtcg
    13621 tccatcagac cgccaagcac gatcagttca ccatcgttag ccagcacggt ggtcttcagt
    13681 ttgcgctcac caaacaccac gtcgaggctg gtctgtcctt ccaccttcga cacttcctgc
    13741 tcaatcacca tctgtaccgc gtttccttcg ttaatctgcg gcgtgacttt cagcatgatg
    13801 ccgacttttt tcctctctac cgtattgaaa ggattgctgt tattggagcc aacggtagat
    13861 ccagttaata ccggaacgtc ctggcccacc atgaagaagg cttcctggtt gtccagcgtg
    13921 gtgatgctcg gcgtggagag cacgttcgag ctggagtcgt ttttgaccgc ctgtaccagt
    13981 gccatccagt cgcctttcac cacgccaacc gccgtaccgc taaagccaga aagcagctgg
    14041 gcgagcgtgg agagatcgcc gttggtatcc gggttaatgg tggtggcacc gttttcactg
    14101 atcaccgtgg agcctttctg cggtttcgcc tgagaaatgg ctgcccccag cgtgccgata
    14161 gggatctgcg taccgttagc aaactgcatt aatccggcat ctttcgacgc ccactgcacg
    14221 ccgaaattga tattgctgcc ttcagcaact tccacgatca acgcctcgac gtgcacctga
    14281 gcacggcgaa tatccagttg ttcaatcacg ctttgcagcg actgcatgat gtcctgcggc
    14341 gcagtaacaa tcagggcatt actgtgtttg ctggcggcga tggagacaac ctcacgcccg
    14401 ctaccgacgg tgccttccgc ctcttcttta gccgccgtga gcgtgccgct gacctgcttc
    14461 agcacatcga ccagatcttc cgctttgctg tatttgagat agaaaacctg gctgttgccg
    14521 ctgcgctcca tttctgagtc cagccgacgg atcaggcggc gcattttgtc ccgcgtggcc
    14581 gggtcaccac tgacaatcac actgttggtg cgttcgtcgg cgacaatttg agatttcagc
    14641 gtcgcaggtt ggttctcgcc gctgtttttc gtcaggcttt ccagcacgcg ggcgatttcc
    14701 gaagccgagg cgttatccag cgggatcacc tcttcagtgc gattacccgc gtgatccaca
    14761 cgctggatca cttccgtcag ccgctccacg acggaggcgc gcccggtgag cataatcacg
    14821 ttggagggat cgtaattaac aacgttgcct gagcctgcgc tgtcaatcat ctggcgcaga
    14881 atcggtgcca gctcgcgtac cgacacattg cgtaccggca cgactttggt aaccatttca
    14941 tcgcccgcgt agttgtcgct gccttcaccg accagcggca gcggctcgac tttcgcggcg
    15001 cttgatttca ccaccttcag cacgtcgttt tccatcggta cgaccgcata cccctgtgct
    15061 tccagcaggt taaggaataa ctggtaatac tggcgttcat tgagcggagt catagtgcga
    15121 atactcactt tcccctgaac gcccggcccc ataatgatgg ttttattaag gttagcgccg
    15181 acggtttcga tgaacgattt caggtcagta tctttaaaat tagcggtgaa agtggcttct
    15241 tccgcccaga ccggtgaact acacaatgcc gctgccagca tcagcggcag taaacgcttt
    15301 tttgttttga ggccagttgt cttcttacgc cagatagaca acgtcatatc acgccaaaac
    15361 acgatgaatg attctcctgg attaaatgcg gttagcgcag tgcgatggaa atgtcgtagc
    15421 gcgcaccctt gcgtaaaacc gtaagttgaa tggaatccat tgaaggtaac tgccgcatca
    15481 gagcaatcat tgctcgtgga tcagtgaaat cctgctgatt tagtgcaatg gcgatatcgc
    15541 cttccctgaa accgctggca tcgaacagag aacgatctgc ccccggcttc actgcataac
    15601 cgacaattcc ctccttacgc acaggcgtaa gctggatata gttaaaaatt ttctgcggat
    15661 ctttcgccag tgcctgacgc acggcagccg ggatctcaac tggcgcactg gcagcaggct
    15721 cagcaacagc ttgctttgct tcgtcgctga cggctttttt gctggtgacc gccacagggg
    15781 ggcgttcctc ctctgccaga ctcagacgtt ccattttccc ctgatagcgc agcatcacat
    15841 ggtcgcggtt gatttcctca atcactgcgt tgtgagagcc aagccgttca ccctgcaaat
    15901 agacctgctg tttgccgcct tcttcaatca ccgcgccggg tctggcacca aaggcgatcc
    15961 cacgcagcac cacattaaga cgcgtttctg ccacaggcgc agattcaggt tgttttaccg
    16021 gtgcggcgac gggttgatat ttgccaaacc agttttgttg gctgactaat tgcacgtcat
    16081 ttttatcgaa cggttttgca tccgcacgga gcggtttatt cgccgatgag gaaaccaccg
    16141 tatattccgc agaaaaggag aaatagcgcc agagtgaata cgccattttt gcagaaataa
    16201 taagcagcat gagccagaac atcccgcgca ccatttttcg cagatgctct ttgtctgtat
    16261 taagagattg tctctggcta agaatgtgac gaattttagt cagccattga atgagataaa
    16321 ttcgtgcgtc acgaaaaaca acccgcgcca atttatatct acccgacgtt atgctttgac
    16381 tattccacag gtggtacgat ccagtttccg ctgatactgg ttacctgtac gtgtatcatt
    16441 aatcgttatg ctgtaattaa ttccctcggt gatcattaat ttcaccgttg ggtcagcgca
    16501 catttgtcgt tgatagctgg ttaaaaaggc gtcaggtgtc tgcgtggtct gagtacccga
    16561 ttcgctgata atggtcattt ttaccgtcgt gccactactt tgcgccagca ctaaggtata
    16621 tcccgcagat ttaatcggca ggttttggct gatattttgc gcctgttttt tcgccagtaa
    16681 actggcgttt tcattatggc tggcgcagcc acttaataat cctgtaacgc tcaacaatag
    16741 cgatattaat accctccctg gcatttgttt tatcgacatg gtaaataatc tctaaggtta
    16801 ttaataagag ttaaaatgtc actttgataa tgacgttgtt atcattaaaa caatgccttt
    16861 agataaagtg ttgctatacc gcccagactt aaacacggtc caaaaggcag tgtggttgat
    16921 cctctttttg taataacggc atatatcagg ccgcagcatg aggcgattaa agcaacgttg
    16981 ggcagtgata acgcccccac ccagccacct aaagcggcga agagtaatac atcgcccatg
    17041 cctaatgctt ctttacgcag aactattccg gctatccagc gcagggagta aaaagtgata
    17101 aatcccacca gtacgccggt gactgcgtct tgtagcgtga gcggactctg ctgcgcccat
    17161 gccgcaatta gtcccgtcca caatacgccc tgagtaaaaa catcgggcag ccattggtta
    17221 tcgaggtcaa tgacgctcgc ggcaatcagc caggcggata atatcatcac cgccagcgcc
    17281 catccacttt ccggccacac caggctcgcc agcaaaaaag cgagtgccgt caataactcc
    17341 accagcggat aacgcttgct gattttcgcc tgacagtcgc ggcagcgccc tttgagcatc
    17401 aaccaggaga gcagcggaat attgtcacga acgcggatag tctgctggca atgcggacag
    17461 tgcgagcgtg gcagcgccag gcttattttt gactgcgtac tcggcatttc accgtgaaac
    17521 tccgccattt gttggcgcag catgatgggg taacgccaaa tcaccacatt caaaaaactg
    17581 ccgataatca atcctccgac ggtagccagg acgggcatcg ccgcggggta ttgctgaaaa
    17641 acatcaaaaa acatggttaa aggttatttg ttgtaacttg ccggatgcgg gcctgcaggt
    17701 gtatgccata cggctttcct ccaggcccga tgcgccttat ttcatgccgg atgcggcgcg
    17761 agcgccttat ccggtataca ggcttactca gctgacatct tatgctcggt aacctgatta
    17821 atggtttccg gcccttgctg cggtttcggc agattaagcg ccgccagcgt ctcgtaagcc
    17881 gactggctca caccgccctc gaagttcatc tcgctcgctc ccggcaactg gtaagcattc
    17941 gcgcccggat tccatttctt aaagaactcc gaaagatccg tctgggcgac ccaggaggca
    18001 cacagcatca gcgtgtccgc tgcgttaccg ttggattcag cacagtagtt cttactgccg
    18061 aacttgttat tgccaacctc atcgccgcgc gctttacggt gcatcaactg gaacaggttc
    18121 cagcctttca tcccttcacg ctcgctgtaa aacttaggca ggtcaccttc tgggtaccac
    18181 tgtttgatat caaagttttt ctctgcccac tctttcagct gtgcgtacat cagcagacgg
    18241 tcccccgcac cgccgcgcgc ccaggcctga ccgttgctct cgtccagata ttccggcgcg
    18301 acggtaatgt cgtcagcgac acggttcatc ttgccgagat agcgatcctg catgtacagc
    18361 gccagcacgt tgttcgccac ttcagttgca cccggtacgt tcagcggtgt ttctgcagcg
    18421 ttatgaccgg cttcgtgcca aatcagccag tcgttcagcg gcgttgtcgg cagcgtggtg
    18481 ctgttcgtcg agaagctgct gttcattacc ggataacccg agtgcgcatc accgatggag
    18541 atctgcacat cgttggcgaa acggtgttta tgccccgtca atgcttcata ggtaaacatc
    18601 cggtggttac cgctcgtttc attgcgaccg tagaagtcat tcatcgagct ggcaaaggta
    18661 tccagatctt tagcgaattc tgctacacca ccagtgaaat tgctggcctc aaggttcttc
    18721 ttcggcgtgg tatagacgaa cgacgcagac tccagctcgc ccagcggcgc cggtgagtcc
    18781 agatcgtttt tccattcgcc gtctttatag aacggcgctt ttactacacc ggtaaaggtg
    18841 aagttagcag aaacatcatc cttactgtcg cccttgatat aaatcagacc accataaggc
    18901 accttgaagg tcacttcacc gttagcctcc agagtatacg ttttagtcac tcttggcgga
    18961 cggttcagcg caacttcatg cttctcacgt ccagtcaggt cgtcagccag cgccacggta
    19021 acagtcactg ggactgacgc cgaagactta atggtgacgt cctgctgggc cggtgcccac
    19081 aggccggttg actgcatgtt acccgcaaac catttggtcg gattcgagta caggctgatg
    19141 ttttccgtaa cgctctcacc ctttgccgat acggatcctg ggtacttctc cacatcaacc
    19201 ttaatgttca gatcccacca ggaacggccc agcatcagac gcgtcagcgg tttttccata
    19261 tagttgagcg gatagcttgg gttcatcatg cccgctttgc tgctaccgtc accgtagatc
    19321 atgttgttat cgaccagcga ttttttcaga tctgcggagc acttggtgcc gcctgcatag
    19381 gcatcattgg cgtagcagtt caggaactcg gtgaacgttt taaagcccag ctcatcttcc
    19441 ttgccctctt cgtaacgata tttcgtatcg ttccacagcc agaccgacat gttctggtac
    19501 agacgttcca gatcaacact gttcagacgc tcacctttac tgcctttggt acggaaataa
    19561 agctcatgct gatacaggcg ctgaatgttg gtgcctaaat ccgccgcctg caccatcgct
    19621 ttcgcggtgt cggcgtcaag attcagttgc gtatagcgcg gaacatacat gccacctgtt
    19681 accggaacat ccgtgcctgg gcggcgctcc aaacagttaa tctcgtaatg gtaagtcgag
    19741 tccttacact cctgtaaccc aggaaacttc tcaaagattt ttgcctttgc cgcttccaga
    19801 gattcttctg ttgtgtattc cgcttcatca ataaaggcat aacgcgttac ctgtttgccc
    19861 tcaacttcct cctgccagct cgcaacttcc agtttcggct tgtcatcagg cttgttgtct
    19921 tgctggtatt tccaggtcac ttcccctgta tttgggtcga tggtgtacgg cggttgcgcg
    19981 ccgtctgcag caggataacg ttcataaacc caaatgccag tcgcgcgacg ctgacgaacg
    20041 cgatccggat acccttgcgg atcgttgttc accaccgatt tgttcagagc cattgacaga
    20101 cccgcggcat ccagcagacg cacaaaactg gacgcgctct cttccttaag attgctcatc
    20161 acgttttcca tgatcagcac cgagccacct ttgttcagat aagcgatcag atcggtcaca
    20221 tcctgctgag tcagcttcgg tttgctggta tctgcacgca gaggcacagc atagggatcg
    20281 ccagaccact gagtcacata ttcaaagccg ttgaggatca gcaacggaat ctcttccgga
    20341 ttcagatcgc catagctggt caactgttta accgtgatac cagtgaaatc ctcatggaaa
    20401 gcaaatggtg cactatttcc caatacctgg cccgcttttt tgaaataaac gttctccagg
    20461 ttggtgccga cagtcatgat gctcttggta tttggctgcc agatgtcatt tgacaagtag
    20521 cgcaggacgt tctgcataaa gtgcttcatg tcatcagaat caccgctgag cgtacactca
    20581 cctttactat taacaccacc gccccaactg taaccgttcg ggcaacgcag gatgctgttg
    20641 tagtgcgggt taccgataac catcagtttg ccttcaccga cttgccccag cgaaataaac
    20701 ggcaggttga aagtcgcagt atcgcgcgta acgttctctg gctgcacaat ggaaggcgct
    20761 tccgtaatgt acgccagctc atttttatcc caggcgcgtt tttcgccaaa cgccagccag
    20821 tagtttttat cattacgcgc catcagaatc gggaatgccg agttggagat atttaccacc
    20881 gcctgaccgc gcgcgttacc ggtgctgcca tagaagttgg tagagtcatg gaagacgtgg
    20941 aacttgctga cagactgata gttcgtatcc acgccccaca gcttgttaat aacgccctga
    21001 atctggccgt cattaacatt gcgcgttgtc agcgagaacc agcgagcctc gttacaaccg
    21061 tcggttttcg cacaaatcgc ggtatcgatc tctttggcct gacccgtctt aaactgctcg
    21121 ataaattcgt taggcagcac aacgttttga tcgccttcat ccagcgtcgc accgttggat
    21181 aacgaaagat tgattatctc gttgatcacg ttgggatatt cggcaaagac cttgcgtaca
    21241 tagtccggaa caacacgagt attattttga ccagtcgtcg aataacgatg aatgagctga
    21301 tcgatatttg ccccgcgaac ttcatcaccc aattcagtca gcgcaatggt cgacttattg
    21361 ccacgtactg agcccagttc aaaggtatcg ataccaaagg agatgtttcg ccccagctaa
    21421 aggagaattt accgttttcg tcagttacgc cacggcctga attggtgtag tagtcaacgc
    21481 cagcaacacc gttccccagg ctatccacca gttggccttc ggaaagaatg atttcagtgg
    21541 gttgatactg ataaaactgt tccgcgttag ccgacacgaa ggacgcgttc agatccggtt
    21601 ttgttccctc tgtcgtgact ggcactaccg ttgaggtatg ggtggacggc gctttatcag
    21661 tcgcagcatt gttttccacc tcttcattga ccagcttgct gaaattgtct gttgccagat
    21721 caatttgctt atacagtttt tcaaatcgcg cgcgatcaac cactgacgag aaagtaagac
    21781 aaagctgttc tgcatctgcg gggcaactgt cgctggacgt caccagagag atggcgttgg
    21841 ttttcttatt ttcagaattc gccagctcct gcgcgtcctc caggctaaac gacactttgt
    21901 caaccgcacg caggctacgc gcagcttctg actgggtgtt gaatgttgca atggtcgtac
    21961 tgcccaccac acaactcacg gtattgcctg gcgtaaaggt aaagccatcg ctggattcac
    22021 cattacaggt agcaccagtt acccgctggc ttccgcccag ggtcagataa cccgttttcg
    22081 taggaacagg ttctggctcc ggttctggct ctggatcagg tgtcggttcc ggcgtaggtt
    22141 ctgggtccgg cgttggctca ggcgtcggct ccgggtttgg tgttggatca ggtttcactt
    22201 ccggcaaaga ccctgttcca gaatctacag gcggcgtatc ggaggaa
//
LOCUS       DAZGQL010000085        21380 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.085, whole genome shotgun sequence.
ACCESSION   DAZGQL010000085 DAZGQL010000000
VERSION     DAZGQL010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21380
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.085"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..2739
                     /locus_tag="ACHKIR_003724"
     CDS             <1..2739
                     /locus_tag="ACHKIR_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA family autotransporter adhesin"
                     /protein_id="HFO9991403.1"
                     /translation="DSLLWNATAGAFSAKHGTNGTDSKITNLLAGTVSSDSTDAINGS
                     QLYGLADSFTSYLGGGADISDAGVLTGPTYTIGGTDYNNVGDALAAINTSFSTSLGDA
                     LLWDATAKGGDGAFSAGRGKDNTASIITNVADGAISSTSSDAINGSQLYDTSKYIADT
                     LGGDAEVNADGTITAPTYAIAGGSYSNVGDALEAIDTTLDDALLWDATANDGNGAFSA
                     AHGKDKTASVITNVANGAISATSSDAINGSQLYTTNKYIADALGGDAEVNADGSITAP
                     TYTIANAEYNNVGDALDALDDNALLWDATANDGAGAYNASHDGKASIITNVADGNIGE
                     GSTDAINGSQLFNTNMMIQQNSEIINQLAGNTSETYIEDNGAGINYVRTNDNGLAFND
                     ASASGIGATAVGYNSVAEGDSSVAIGQGSYSDVDTGIALGSSSVSSRVIVKGSRDTSV
                     SEEGVVIGYDTTDGELLGALSIGDDGKYRQIINVADGSEAHDAVTVRQLQNAIGAVAT
                     TPTKYFHANSTAEDSLAVGEDSLAMGAKTIVNGNAGIGIGLNTLVLADAINGIAIGSN
                     ARANHANSIAMGNGSQTTRGAQTGYTAYNMDAPQNSVGEFSVGSEDGQRQITNVAAGS
                     ADTDAVNVGQLKVTDAQVSQNTQSITNLNTQVTNLDTRVTNIENGIGDIVSTGSTKYF
                     KTNTDGVDANAQGKDSVAIGSGSIAAADNSVALGTGSVANEENTISVGSSTNQRRITN
                     VAAGKNATDAVNVAQLKSSEAGGVRYDTKADGSVDYSNITLGGGNGGTTRISNVSAGV
                     NNNDAVNYAQLKQSVQETKQYTDQRMVEMDNKLSKTESKLSGGIASAMAMTGLPQAYT
                     PGASMASIGGGTYNGESAVALGVSMVSANGRWVYKLQGSTNSQGEYSAALGAGIQW"
     gene            3151..4806
                     /gene="lldP"
                     /locus_tag="ACHKIR_003725"
     CDS             3151..4806
                     /gene="lldP"
                     /locus_tag="ACHKIR_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFO9991404.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVIYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            4806..5582
                     /gene="lldR"
                     /locus_tag="ACHKIR_003726"
     CDS             4806..5582
                     /gene="lldR"
                     /locus_tag="ACHKIR_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HFO9991405.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGEKEKIQLCFEATLSEDPDLASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGDHNEHSREKNA"
     gene            5579..6769
                     /gene="lldD"
                     /locus_tag="ACHKIR_003727"
     CDS             5579..6769
                     /gene="lldD"
                     /locus_tag="ACHKIR_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HFO9991406.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALGPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIREFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPAALAPMAK
                     GNAA"
     gene            6842..7315
                     /gene="trmL"
                     /locus_tag="ACHKIR_003728"
     CDS             6842..7315
                     /gene="trmL"
                     /locus_tag="ACHKIR_003728"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFO9991407.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPTEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGAVLRD"
     gene            complement(7368..8189)
                     /gene="cysE"
                     /locus_tag="ACHKIR_003729"
     CDS             complement(7368..8189)
                     /gene="cysE"
                     /locus_tag="ACHKIR_003729"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFO9991408.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(8269..9288)
                     /gene="gpsA"
                     /locus_tag="ACHKIR_003730"
     CDS             complement(8269..9288)
                     /gene="gpsA"
                     /locus_tag="ACHKIR_003730"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFO9991409.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(9288..9755)
                     /gene="secB"
                     /locus_tag="ACHKIR_003731"
     CDS             complement(9288..9755)
                     /gene="secB"
                     /locus_tag="ACHKIR_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HFO9991410.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(9818..10069)
                     /gene="grxC"
                     /locus_tag="ACHKIR_003732"
     CDS             complement(9818..10069)
                     /gene="grxC"
                     /locus_tag="ACHKIR_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HFO9991411.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(10211..10642)
                     /locus_tag="ACHKIR_003733"
     CDS             complement(10211..10642)
                     /locus_tag="ACHKIR_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFO9991412.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVIVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            10887..12431
                     /gene="gpmM"
                     /locus_tag="ACHKIR_003734"
                     /gene_synonym="pgmI"
     CDS             10887..12431
                     /gene="gpmM"
                     /locus_tag="ACHKIR_003734"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HFO9991413.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVEGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            12465..13724
                     /gene="envC"
                     /locus_tag="ACHKIR_003735"
     CDS             12465..13724
                     /gene="envC"
                     /locus_tag="ACHKIR_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418070.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HFO9991414.1"
                     /translation="MTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADERDQLKSIQ
                     ADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNKQIDEMNAS
                     IAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYFGYLNQARQ
                     ETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKKTLAGLESS
                     IQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEATRKGTTYK
                     PTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIGASEGTEVK
                     AIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQPIALVGSS
                     GGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            13728..14687
                     /locus_tag="ACHKIR_003736"
     CDS             13728..14687
                     /locus_tag="ACHKIR_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HFO9991415.1"
                     /translation="MFPFRRNVLAFAALLALSSPVIAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNTVPDTPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKLNASVN"
     gene            complement(14674..15708)
                     /gene="waaH"
                     /locus_tag="ACHKIR_003737"
     CDS             complement(14674..15708)
                     /gene="waaH"
                     /locus_tag="ACHKIR_003737"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HFO9991416.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWCFRETGETWQSIPTDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLNRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDIITRQRMIAEIFTSGMYKRLITNVRSVKVGYQALLWSFRLWQWRDKTRSHH
                     RITRSAFNLR"
     gene            complement(15947..16972)
                     /gene="tdh"
                     /locus_tag="ACHKIR_003738"
     CDS             complement(15947..16972)
                     /gene="tdh"
                     /locus_tag="ACHKIR_003738"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFO9991417.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARYVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(16982..18178)
                     /gene="kbl"
                     /locus_tag="ACHKIR_003739"
     CDS             complement(16982..18178)
                     /gene="kbl"
                     /locus_tag="ACHKIR_003739"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFO9991418.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGQVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            18392..19324
                     /gene="rfaD"
                     /locus_tag="ACHKIR_003740"
     CDS             18392..19324
                     /gene="rfaD"
                     /locus_tag="ACHKIR_003740"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HFO9991419.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            19334..20380
                     /gene="rfaF"
                     /locus_tag="ACHKIR_003741"
     CDS             19334..20380
                     /gene="rfaF"
                     /locus_tag="ACHKIR_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HFO9991420.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            20384..21364
                     /gene="rfaC"
                     /locus_tag="ACHKIR_003742"
     CDS             20384..21364
                     /gene="rfaC"
                     /locus_tag="ACHKIR_003742"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HFO9991421.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGMDWQTAREPLASLFYNRKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQMVCRAPGNELSQLTANAVKQFIEENAEKAAMI"
BASE COUNT         5197 a         5657 c         5608 g         4918 t
ORIGIN      
        1 gattccctgc tgtggaacgc tacggcgggt gcattcagtg ccaaacacgg caccaacggt
       61 actgacagca aaatcaccaa cttactggca ggcactgtat cctctgatag cactgacgcc
      121 attaatggct cacaacttta tggtttagct gattcattta cgtcatacct tggcggtggt
      181 gctgatatca gcgatgcggg tgtattaacc gggccaacct acactattgg tggtactgac
      241 tacaataacg tcggtgatgc tctggcagcc attaacacgt catttagcac atcactcggt
      301 gacgccctac tctgggatgc gaccgccaag ggcggtgacg gtgcgtttag cgccggtcgc
      361 ggaaaagata acacggccag catcattact aatgttgcag acggtgcaat ttcctctacc
      421 agcagcgacg ctattaacgg ctcacagctc tatgacacca gtaagtacat tgccgatact
      481 ctgggtggtg acgcagaagt caatgctgac ggcacaatca ccgcaccgac ttatgctatc
      541 gctggcggca gttacagcaa cgtcggtgac gcgctggaag caatcgatac cacgctcgat
      601 gacgctctgt tgtgggatgc cacagccaat gatggtaatg gtgcatttag cgccgctcac
      661 ggaaaagata aaacagccag tgtaatcact aacgtcgcta acggtgcaat ctctgccacc
      721 agcagcgatg ccattaacgg ttcacaactg tataccacca acaagtacat tgccgatgcc
      781 ctgggtggtg acgcagaagt taacgctgat ggttctatca ctgcgccgac ttacaccatt
      841 gcaaatgccg agtacaacaa cgtcggtgac gccctggatg cgctcgacga taacgctctg
      901 ctgtgggatg caacggccaa tgacggcgca ggtgcctaca acgccagcca tgacggcaag
      961 gccagcatca tcacaaatgt tgctgatggt aacattggcg aaggcagtac cgacgccatc
     1021 aacggttctc agctgtttaa caccaatatg atgatccagc agaacagcga aatcatcaat
     1081 cagcttgccg gtaacaccag cgaaacctac atcgaagata acggtgcggg tattaactat
     1141 gtacgtacca acgacaacgg cttagcgttc aacgatgcca gcgcttcagg tattggcgct
     1201 acggctgtgg gttataactc tgttgccgaa ggcgatagca gcgtagccat cggtcagggc
     1261 agctacagcg acgttgatac cggtattgcc ctgggtagca gctccgtttc cagccgtgta
     1321 atagttaaag gttctcgtga caccagcgtg tcggaagaag gtgttgtgat tggttatgac
     1381 accacggatg gcgaactact tggtgcgctg tctattggtg atgacggtaa atatcgtcaa
     1441 atcatcaacg tagccgatgg ttccgaagcc catgacgccg ttacggttcg ccagttgcaa
     1501 aatgccattg gtgcagtcgc taccacgccg accaaatact tccacgccaa ctcaacggct
     1561 gaagactcac tggctgtcgg ggaagactcg ctggcgatgg gcgcgaaaac tatcgtcaat
     1621 ggtaatgcgg gtattggtat cggcctgaat actctggttc tggcagatgc catcaacggt
     1681 attgctatcg gttctaacgc acgcgccaac catgcaaaca gtattgcaat gggtaatggt
     1741 tctcagacca ctcgcggcgc tcagactggc tacaccgcct acaacatgga tgcaccgcag
     1801 aactctgtgg gtgaattctc cgtaggtagc gaagacggtc aacgtcagat caccaacgtc
     1861 gcagcggggt cggcggatac cgatgcggtt aacgtgggtc agttgaaagt aacggacgcg
     1921 caggtttccc agaataccca gagcattact aacctgaata ctcaggtaac gaatctggat
     1981 acccgcgtca ccaatatcga aaacggtatt ggcgatatcg taagcaccgg tagcaccaag
     2041 tacttcaaga ccaacaccga tggcgtagat gctaacgcgc agggtaaaga cagtgttgcg
     2101 attggctctg gctctattgc tgccgctgat aacagcgttg cgctgggtac tggctctgtg
     2161 gcaaacgaag aaaatacgat ctccgtaggt tcttccacta accaacgccg tatcaccaac
     2221 gttgccgctg gtaaaaatgc tactgacgcg gttaacgttg cgcagttgaa gtcttccgaa
     2281 gcaggcggcg tgcggtatga caccaaagct gacggttctg ttgattacag caacatcact
     2341 ctcggcggtg gtaatggcgg aacgactcgc atcagcaacg tttccgctgg cgttaacaac
     2401 aacgacgcgg tgaactacgc gcagttgaag caaagcgtgc aggaaacgaa gcaatacacc
     2461 gatcagcgca tggttgagat ggataacaaa ctgtccaaaa ccgaaagcaa gttgagcggt
     2521 ggtatcgctt ctgcaatggc gatgaccggt ctgccgcagg cttacacgcc aggtgccagc
     2581 atggcctcta ttggtggcgg tacttacaac ggtgaatcgg ctgttgcttt aggtgtgtcg
     2641 atggtgagcg ccaatggtcg ttgggtctac aaattacaag gtagtaccaa tagccagggt
     2701 gaatactccg ccgcgctcgg tgccggtatt cagtggtaat catccattaa cgaatgggta
     2761 aacgccacat tgcctgatgc gctacgctta tcaggcctac ggggtgattc agcactaccc
     2821 tgtaggccgg ataaggcgtt cacgccgcat ccggcaagat aaggcgcacc agatcaacaa
     2881 tctgagggca attctctgat gggaattgcc cttttcttca ttacctctct cccagaattg
     2941 gccctaccaa ttcttcattg atctgacctc tggttcacaa ttccccaatt aaaactcaca
     3001 tcaccgttgc caatacataa catctagtta actattcatt gtcattatcc ctacacaaca
     3061 caattggcag tgccactttt acacaacgtg tgacaaggag atgagcaaca gactcattac
     3121 acggcgtgtg tggactgcag gagacctgca atgaatctct ggcaacaaaa ctacgatccc
     3181 gccgggaata tctggctttc cagcctgata gcatcgcttc ccatcctgtt tttctttttt
     3241 gcgctgatta agctcaaact gaaaggatac gtcgccgcct catggacggt ggcaatcgcc
     3301 cttgccgtgg ctttgctgtt ctataaaatg ccggtcgcta acgcgctggc ctcggtgatt
     3361 tatggcttct tctacggttt gtggcccatc gcgtggatca ttattgcggc agtgttcgtc
     3421 tataagatct cggtgaaaac cgggcagttt gacatcatcc gctcgtctat tctttcgata
     3481 acccctgacc agcgcctgca aatgctgatc gtcggtttct gtttcggcgc tttccttgaa
     3541 ggagccgcag gctttggcgc tccggtagca attaccgccg cattgctggt cggtctgggc
     3601 tttaaaccac tctacgccgc cggactgtgc ctgattgtta acaccgcgcc agtggcattt
     3661 ggtgcgatgg gtattccgat tctggtcgcc gggcaggtaa caggcatcga cagctttgag
     3721 attggtcaga tggtggggcg tcagttgccg tttatgacca ttatcgtgct gttctggatc
     3781 atggcgatta tggacggttg gcgcggaatc aaagaaacgt ggcctgcggt cgtggtggca
     3841 ggtggttcgt ttgccattgc tcaatacctc agctctaact tcattgggcc ggaactgcca
     3901 gacattatct cttcgctggt atcactgctc tgcctgacgc tgttcctcaa acgctggcaa
     3961 ccagtgcgcg tattccgttt cggtgacttg ggggcgtcac aggttgatat gacgctggct
     4021 cacaccggtt acaccgcagg tcaggtgctt cgcgcctgga caccgttcct gttcctgacc
     4081 gccaccgtaa cgctgtggag tatcccgccg tttaaagccc tgttcgcttc aggcggtgcg
     4141 ctgtatgagt gggtgatcaa cattccggtg ccgtacctcg ataaactggt tgcccgtatg
     4201 ccgccagtgg tcagcgaagc gacagcgtat gccgccgtgt ttaagtttga ctggttctct
     4261 gccaccggta ccgccattct ttttgctgcc ctgctctcaa ttgtctggct gaagatgaaa
     4321 ccatctgacg ctatcagcac cttcggcagc acgctgaaag aactggctct gcctatctac
     4381 tccatcggta tggtgctggc gttcgccttt atctcgaact attccggact atcatcaacg
     4441 ctggcgctgg cactggcgca caccggccat gcattcacct tcttctcgcc gttcctcggc
     4501 tggcttggtg tcttcctgac cggatcggat acctcatcta acgccctgtt cgccgccctg
     4561 caagccactg cagcacaaca aattggcgtt tctgacctgt tgttggttgc cgccaacacc
     4621 accggtggtg tcaccggtaa gatgatctct ccgcaatcta tcgctatcgc ctgtgcggcg
     4681 gtaggcctgg tgggcaaaga gtctgatttg ttccgcttta ctgtcaaaca cagcctgatc
     4741 ttcacctgta tggtgggcgt gatcaccacg cttcaggctt atgtcttaac gtggatgatt
     4801 ccttaatgat tgttttaccc agacgcctgt cagacgaggt tgccgatcgt gtgcgggcgc
     4861 tgattgatga aaaaaacctg gaagcgggca tgaagttgcc cgctgaacgt caactggcga
     4921 tgcaactcgg cgtgtcgaga aattcactgc gcgaggcgct ggcgaaactg gtcagtgaag
     4981 gtgtgctgct cagtcgacgt ggcggcggga cgtttattcg ctggcgtcat gatacatggt
     5041 cggagcaaaa catcgtccag ccgctgaaaa cgctgatggc cgatgatccg gattacagtt
     5101 tcgatattct ggaagcccgc tacgccattg aagccagcac cgcgtggcat gcggcaatgc
     5161 gcgccacacc aggcgaaaaa gagaaaattc agctttgctt tgaagcaacg ctaagtgaag
     5221 acccggatct cgcctcacaa gcggacgttc gttttcatct ggcgattgcc gaagcctcac
     5281 ataacatcgt gctgctgcaa accatgcgcg gtttcttcga tgtcctgcaa tcctctgtga
     5341 agcatagccg ccagcggatg tatctggtac ccccggtttt ttcacagctg accgaacaac
     5401 atcaggcagt cattgacgcc atttttgccg gtgatgctga cggggcgcgt aaagcaatga
     5461 tggcgcacct tagctttgtt cacaccacca tgaaacgatt cgatgaagat caagctcgcc
     5521 atgcacgcat tacccgcctg cctggtgacc ataatgagca ttcgagggag aaaaacgcat
     5581 gattatttcc gcagccagcg attatcgcgc cgcagcacaa cgcattctgc cgccgttcct
     5641 gttccactat atggatgggg gtgcatattc tgaatacacg ctgcgccgca acgtggaaga
     5701 tttgtcagaa gtggcgctgc gccagcgtat tctgaaaaac atgtctgact taagcctgga
     5761 aacgacgctg tttaatgaga aattgtcgat gccggtggcg ctaggtccgg taggtttgtg
     5821 tggcatgtat gcgcgacgcg gcgaagttca ggctgccaaa gccgcggatg cgcatggcat
     5881 tccgtttact ctctcgacgg tttccgtttg cccgattgaa gaagtggctc cagccattaa
     5941 acgtccgatg tggttccagc tttatgtgct gcgcgatcgc ggctttatgc gtaacgcact
     6001 ggagcgagca aaagcggcag gctgctcgac actggttttc accgtggata tgccgacacc
     6061 gggcgcgcgt taccgtgatg cacactcggg tatgagcggc ccaaatgcgg caatgcgccg
     6121 ttacttgcag gcggtgacgc atccgcaatg ggcgtgggat gtgggcctga acggtcgtcc
     6181 acatgattta ggtaatatct cggcttacct cggcaaaccg accggactgg aagattacat
     6241 cggctggctg gggaataact tcgatccgtc catctcatgg aaagaccttg agtggatccg
     6301 cgaattctgg gatggtccga tggtgatcaa agggatcctc gatccggaag atgcgcgcga
     6361 tgcagtacgt tttggtgctg atgggattgt ggtttctaac cacggtggcc gccagctgga
     6421 cggtgtactc tcttccgctc gtgcattgcc cgctattgcg gatgcggtga aaggtgatat
     6481 tgccattctg gcagatagcg gaatacgtaa cgggcttgat gtcgtgcgta tgattgcgct
     6541 cggtgccgac accgtactgc tgggtcgtgc tttcctgtat gcgctggcaa cagcgggcca
     6601 ggcgggcgtg gcaaatctgc tgaatctgat cgaaaaagag atgaaagtgg caatgacgct
     6661 gactggcgcg aaatcgatta gcgaaattac gcaagattcg ctggtgcagg ggctgggtaa
     6721 agagttgccg gcggcactgg ctccgatggc gaaagggaat gcggcatagt tgggggtgag
     6781 gggaaaaggc cgtaccaatc accaattttc tgctatcctg cccccgcact aagggggcag
     6841 tatgctaaac atcgtacttt acgaaccaga aattccgcca aatactggca acatcatccg
     6901 tctttgcgct aataccggct ttcgtctgca tatcatcgaa ccaatgggat ttgcctggga
     6961 cgataagcgc ctgcgccgcg cggggctgga ctatcacgag tttaccgccg ttacgcgtca
     7021 tcatgactat cgcgcgttcc tcgaagcaga aaatccccag cgcctgtttg ccctcaccac
     7081 gaaaggtacg cctgctcaca gcgccgtaag ctatcaggat ggcgattatc tgatgttcgg
     7141 cccggaaaca cgcggcctgc cagcgagcat tcttgatgcc ctgcccactg aacaaaaaat
     7201 tcgtattcca atggtgccgg acagccgcag catgaacctg tctaatgcgg tgtcggtcgt
     7261 ggtgtatgaa gcctggcggc agttggggta tccgggagcg gtgttgagag attagttact
     7321 gtaggccgga tagatgatta catcgcatcc ggcacgatca caggacatca gatcccatca
     7381 ccatactcaa acgtatggtt aatgccgttg aaatgctgat ccatatccat tgacggttta
     7441 tcgctatccg gcttgccgac aatgcgtgcc ggaacgccag cggcagtggt gtgcggcggc
     7501 accggttgca gcaccacgga acctgcgcca atcttcgcgc cgcgcccaac ttcaatatta
     7561 ccgagaattt tcgcgcccgc gccaatcatc acgccttcac gaattttcgg gtgacggtca
     7621 ccaccagatt tacccgtacc gccaagcgtc acagattgca gaatcgatac gtcgttttca
     7681 atcaccgccg tttcacccac aacgatgccc gtcgcgtggt cgagcatgat accgcgacca
     7741 atttttgctg ccgggtgaat atcaacctgg aacgttacag agacctggtt ttgtagaaaa
     7801 atagccagcg cgcgacggcc ctgattccac aaccagtgac cgatgcgata cgcctgcaaa
     7861 gcatgaaaac ccttcaggta tagcaacggg gttgagtatt tatccaccgc cgggtcacgg
     7921 gtacgcaccg cctgaatatc acaggccgca gaggcgatca tttccgggtc ggcggcgtag
     7981 gcttcttcca ccacttcacg gatagcaata gccggcataa tgggcgatga cagcttgttc
     8041 gccagcatgt agctcagtgc actgccaagg ttttcgtgct tgagtagcgt cgcgtggtaa
     8101 aaactggcca gcattggctc acagtctgcc agcgttctgg cttcagcttt aatattgttc
     8161 cagacaattt ccagttcttc acacgacatt gcttactcca cacgataaga taatgaccgg
     8221 cccgttctgc gcgggccggg tcatagtggt aacaaaggtt ccctgggttt agtggctgct
     8281 gcgctcgtcc ttgcgtgcac gacctagtaa agtcaatgct gcctcgcgcg cgttttttcc
     8341 gcaatataat acttgataaa tttcctcggt tattggcatt tcaacgccga agcgatgcgc
     8401 cagttcgcgg acttctttcg tattgcggta gccttccacc acttgaccaa ttttctcctg
     8461 cgcgctttgt acatccatgc cctgaccgag catcatgcca aaacggcggt tacgcgactg
     8521 gttgtcggta caggtcagca ccagatcgcc cagccccgcc atgcccataa aggtggcagg
     8581 atcggcaccc agcgccgcgc caagacgcga catctcagcc agcccacggg tgatcagcgc
     8641 cgtacgcgca ttcgcgccaa aaccgatacc gtcggacatc cccgcgccaa tggcaatgac
     8701 gtttttcacc gcgccgccaa gctgcacacc gatgaaatcc gggttgctgt aaacgcggaa
     8761 acttttgcca cagtgcagca attgctggag gtcatcggca aaggtctggt ccgtcgaggc
     8821 cagcgaaatg gctgtcggta aacctgccgc cagttctttc gcaaacgttg ggccagagat
     8881 aaccgccagc ggaatttgat cgcctaaggc ctcacgcgct acgtcctgta acagacgtcc
     8941 ggtttctgct tccagccctt tggtcgccca caccagacgc gcatcaggac gcatcagcgg
     9001 tttaatctgg cgcagcactt caccaaagac atggctgggt acgacgacga gaatattacg
     9061 gctggctgcc agcgcagtgg cgagatcgct ttcaagatgg agcgtatcgg gaaaaggcac
     9121 atcggggaga aacgcggcgt tacagcggtc gcgttcaagc gttgcgatat gttcagggtc
     9181 atggccccag aggacaacct cgtggccatt tcttgccagg gtaatggcaa gagcggtgcc
     9241 gtacgagccg gcaccgatca cagtcattga agcattacgt tggttcatca ggcatcctga
     9301 tgttcttcag taccttcgcc agcctgctgc tgcaaatagt tcatgaacag cgcatcgaag
     9361 ttaaccggcg caaggttcag ttgcgggaat gtaccgcggg ataccatgct ggtgatgcac
     9421 tcacgagcat acgggaacag aatgttcggg cagtatgcac ccaggcaatg tgccatctgg
     9481 gtgccttcga tacccgcgat ggagaaaata ccgccctgct gaacttcaca caggaacgcg
     9541 gtttcttcgc ccagggaggc cgttacggta acacgcagta ccacttcgta aacgtcatct
     9601 gccagttgag aagatgccgt atccagatca agtttaactt ctggctgcca atctttctgg
     9661 aaaacgtgcg gcgcgttcgg cgcttcgaaa gaaatatcct tggtataaat acgttggatc
     9721 tggaaagtca tttcggtgtt gttttgttct gacatgtgta gaaaaccctt aagtgttgtc
     9781 cttaaatact gcgcaatgta atgccaacgt tcacacgtta tttcagcagg ggatccagtc
     9841 caccacgtgc atccaatgca tacaagtcat cacagccgcc aatgtgctgt gcgtcaataa
     9901 aaatctgggg aaccgtggtg cgaccgctgc gtttgatcat ctcttcacgc ttagcggcgt
     9961 tgccatcgat cggcaactcc tgaaaactca cgcccttgct gctcagcagt gctttcgcac
    10021 gatggcaata cgggcaggtt tctttggtat agatttcaac attggccata acttagctcc
    10081 tgttttttta ccctgaaatt tcatgttgca gggcggctgc aaaatggagt gtccccagaa
    10141 gcttataaca ataagtcgct gggggcctaa ttgcagctaa cggcctgcat catgaaagac
    10201 gacaggtaaa ttatttgccg cgcaccagag gtaagttttc gccagcccag ccagcaacgc
    10261 cctctttcag cacgaatact tgcgcaaaac cagctttcgt cagcgcgttt gcaggctcct
    10321 ggcactgcat gccagaaccg tctaccacga taaccggttt gtctttgtgc ttctcaagct
    10381 caccaacatt gttggctttg atttcgctcg gcaacaggtt gatagaacct gcgatatggc
    10441 ctttacggaa gtcatcacgc tgacgtaaat ccacaaccac agcatcttct ttgttgatca
    10501 gacgcgtagc ttcaccacga gtaatcacct tcactttcga ggtcaggctc ttaaacgtag
    10561 tcacaataac cgccaccagt aacgcgatcc aggcgatact cagtatggga tgacggccaa
    10621 caaattgcat aatttcttgc atggggggta acaactcccg acgtagtgat taaaaaaacc
    10681 aggaaaggag tatacctgcg cggtgcagca aatacagcca gcgcgttaac tggaatgcaa
    10741 ttttgcgggg cgcgacgaaa aaaaagcgca aatctggcat cggcttgctg cgagcggtcg
    10801 tatattttga tcttcagagg ctattttatc gattcagctg tagtaaaatt acgcaaattt
    10861 tgactcttga gtatgaggtt gtcgcaatgt cggtttctaa aaaacctatg gtactggtga
    10921 ttctggatgg ctatggctat cgtgaagaac agcaggataa cgccattttt agtgctaaaa
    10981 ccccggtaat ggatgcactg tgggccaatc gtccgcatac cctgatcgac gcttccggtc
    11041 tggaagtcgg tctgcctgac cgtcagatgg gtaactccga agtaggtcac gttaacctgg
    11101 gtgccggccg catcgtgtat caggacctga ctcgtctgga cgttgaaatc aaagatcgcg
    11161 ctttcttcgc taacccggta ctgactggcg cggtagataa agcgaaaaac gcaggcaaag
    11221 cagttcacat tatgggtctg ctctccgcag gtggtgtaca cagccacgaa gatcacatca
    11281 tggcgatggt agaactggca gctgaacgcg gcgcagaaaa aatctacctg cacgcattcc
    11341 ttgacggtcg cgacactccg ccgcgcagtg ctgaatcctc gctgaaaaaa ttcgaagaga
    11401 aatttgccgc actgggcaaa ggccgcgtag cgtccatcat tggtcgttac tacgccatgg
    11461 accgcgataa ccgttgggat cgcgttgaaa aagcttatga cctgctgact ctggcgcagg
    11521 gcgagttcca ggccgatacc gccgttgctg gtttgcaggc tgcttatgct cgcgacgaaa
    11581 acgacgaatt cgtgaaagcg accgttattc gtgcagaagg tcagccagac gcggcaatgg
    11641 aagacggcga tgcgctgatt ttcatgaact tccgtgctga ccgtgcacgt gaaatcactc
    11701 gtgcattcgt aaatgccgat tttgacggct tcgcgcgtaa gaaagtggtt aacgtcgatt
    11761 tcgtgatgct gaccgaatac gctgccgaca tcaaaaccgc agtggcttac ccacccgctt
    11821 ccctggttaa caccttcggc gagtggatgg cgaaaaacga caaaactcag ctgcgtattt
    11881 ccgaaaccga aaaatatgcc cacgttactt tcttcttcaa cggtggcgta gaagagtcgt
    11941 tcaaaggcga agatcgcatt ctgatcaact caccgaaagt ggctacctac gatctgcaac
    12001 cggaaatgag ctccgcagag ctgaccgaaa aactggttgc ggcaatcaag agcggcaaat
    12061 acgacaccat tatctgtaac tatccgaacg gcgacatggt aggtcacacc ggagtgatgg
    12121 aagcggcggt taaagcggtt gaagcgctgg atcactgcgt ggaagaagtc gcgaaagcgg
    12181 ttgagtccgt gggtggacaa ctgctgatca ccgctgacca cggtaacgct gagcagatgc
    12241 gcgatccggc aacgggtcag gcacacacgg cacataccaa cctgccagtt ccgctgattt
    12301 acgttggcga taagaacgtg aaagcggttg aaggcggcaa actttctgat atcgcgccga
    12361 ccatgttgtc gctgatgggt atggaaatcc cgcaagagat gactggtaag ccgctgttca
    12421 tcgtggaata atccctcccc atgaggggaa aggcgattaa taccatgaca cgggccgtga
    12481 aaccgcgcag gtttgcaatc aggcccatca tctacgccag cgttcttagc gctggcgtat
    12541 tgttgtgcgc cttttccgcc cacgcggatg agcgtgacca actcaaatct attcaggccg
    12601 atatcgccgc gaaagagcgc gcggtacgcc aaaagcaaca acaacgcgca agcctgctcg
    12661 cacaattgaa aaagcaggaa gaagcgatct cggaagccac ccgtaagctg cgtgaaacgc
    12721 aaaacacgct caatcaactg aataaacaga ttgatgagat gaacgcgtcg attgccaaac
    12781 tggagcagca aaaagccgcc caggagcgca gcctcgccgc acaactagat gccgcattcc
    12841 gtcagggcga gcataccggt attcagctga ttctcagcgg tgaagaaagc cagcgtggac
    12901 agcgtttaca ggcttatttc ggctatctca accaggcgcg acaagaaacc attgctcagt
    12961 tgaaacaaac gcgtgaagaa gtcgctatgc aacgcgccga gctggaagag aaacagagcg
    13021 agcaacaaac gctgttgtat gagcagcgcg cccaacaggc gaagctgacc caggcgctga
    13081 acgagcgtaa aaagacgctg gcagggctgg agtcttccat ccagcaaggt cagcaacagt
    13141 tgagcgagct gcgcgccaac gaatcccgtc tgcgtaacag cattgcccgt gcggaagccg
    13201 cggcgaaagc gcgtgctgaa cgagaagcgc gtgaggccca ggcggttcgc gaccgccaga
    13261 aagaagcgac gcgcaaaggc accacctaca aaccgaccga aagcgaaaaa tcgctgatgt
    13321 cccgaactgg tggcctgggt gcgccgcgtg gtcaggcatt ctggccagtt cgcgggccga
    13381 cgctgcatcg ctatggcgag cagctacagg gtgaattacg ctggaaagga atggttatcg
    13441 gtgcctctga aggtactgaa gttaaagcca ttgccgacgg tcgggtgatt ctggctgact
    13501 ggctgcaagg ctacggtctg gtggtggtgg ttgagcatgg taaaggcgac atgagtcttt
    13561 acggctataa tcagagcgca ctggtgagcg ttggttcgca ggttcgcgcg ggccagccga
    13621 ttgcactggt gggcagcagt ggcggtcagg gtcggccttc actctatttc gaaattcgcc
    13681 gccagggtca ggcggtcaat ccacagccgt ggttgggaag ataagttttg tttccatttc
    13741 gtcgtaacgt tcttgcattt gccgctctgt tggcgctctc ctcccccgta attgctggca
    13801 agcttgccat cgtcattgat gattttgggt atcgcccgca caacgaaaac caggtgctgg
    13861 cgatgccttc cgctatctcc gtcgctgtat tacccgattc accgcacgcc agagagatgg
    13921 caaccaaagc gcataacagc ggtcacgaag tgttgattca tctaccgatg gcaccactga
    13981 gtaaacagcc gctggagaaa aatacgctac gcccggagat gagcagcgac gaaattgagc
    14041 gcattattcg tagtgcggtc aataacgtgc cctatgccgt ggggatcaac aaccacatgg
    14101 gcagcaagat gacctctaac ctgtttggta tgcagaaagt gatgcaggcg ctggagcgtt
    14161 acaatcttta cttcctcgac agcgtaacca tcggtaatac ccaggcgatg cgcgcagcgc
    14221 aaggcactgg cgtgaaggtg atcaaacgga aagtgttcct cgacgattcg caaaatgaag
    14281 cggacatccg tgtgcagttc aatcgcgcaa ttgatctggc gcgtcgcaac ggttcaacca
    14341 ttgccattgg gcatcctcac ccttcaacgg tacgcgtgct acaacagatg gtttataacc
    14401 tgccgccaga cattacgctg gtgaaagcca gcagcttgct gaatgaaccg caggttgata
    14461 cgtctacacc gccgaaaaac actgtgcccg acacaccgcg taatccgttc cgtggcgtga
    14521 agctctgcaa accgaagaaa ccgatagaac ctgtttatgc taatcgcttc tttgaagtat
    14581 taagcgaaag catcagccag agcacgctga tcgtttactt ccaacatcag tggcaaggct
    14641 ggggcaaaca gcctgaagcg gcaaaactta acgctagcgt aaattaaagg cgctacgcgt
    14701 aatgcgatgg tgcgaccgcg ttttgtcgcg ccattgccat aagcggaaag accacagtaa
    14761 cgcctgataa cccaccttca cgctgcgcac attggtaatc aggcgcttat acataccgga
    14821 agtaaatatc tcggcaatca tccgttggcg ggtaataata tccggctctt tgcgcaccgc
    14881 atggcaaaca cgcaatgctt cgtaagttat ttgctgatga aattccggat aaatcgtaat
    14941 tttgtcggca taatttcgat ttaatttctc tagcaggcgg gtaatcttaa tatagtgacg
    15001 ttgataatta aggtttttat tcccttgtct attcaaccga ctcactgacg tattatgcag
    15061 ataatattta tataacgatt gctcggtata tcgcgcacgc agcgcattaa acataaattc
    15121 tgttgtccag acaatatcct gatgatgtaa tccggcaata aatttaatgt tatttttaac
    15181 aataacatcg cggcgataaa cccccatcca gacaacgtga gtccaacggc gggaagaaag
    15241 ccccatccgc aaccaatccg ggccggttaa tacgccggtt gagcgaaggc gatcggtggg
    15301 gatggattgc caggtttctc ccgtttcacg aaaacaccag tcagcgttgc actgcgccac
    15361 gtcgaggtcg tcctctaacg ccatggtcat cagcgtttcg tacatggtgg gatagacctc
    15421 atcgtcagca tcgacaaaag cgacatattt gcccgtcgcc acctcgatcc cacgattacg
    15481 cgctaccgat gcgccagcat tcgcctgatg caacaaacga acgtgcggat agttttctgc
    15541 gtaatgcttt gctatttcaa cagaattatc cgttgaacca tcgttaataa taatgatttc
    15601 cagagcagtc caggtttgcg taattaaaga ttccatacac gtgcggaaat catcgcccgc
    15661 attatataac gggataataa cactaagttt attggtgctg ttcatcataa attaccgacg
    15721 acctctgaag ttatcctgac gttttacagg ctttgtatta agaaactatt aagcctgtga
    15781 gatattattt ttacaggtga gagagtcact tcagggtaga gagataatga gaacagctat
    15841 ataagagatc agcgcaatta cgaactcttt attacactga caggatttta catatttaac
    15901 ggattatcct caggaaaaag ggctggaata ccagcccttg tttgtgttaa tcccagctca
    15961 ggataacttt cccggactgg cccgaacgca tggcgtcaaa gcccttctgg aaatcatcga
    16021 tagagaaacg atgagtaatg atcggcgaaa gatcgaggcc agactgaatc agcgccgcca
    16081 tcttgtacca ggtttcaaac atctcacgac cgtaaatacc tttaatgaac aagcctttaa
    16141 agatcacttt ggtccagtct atcgacatgt cagaaggtgg aatacccagc atcgcaatac
    16201 gaccgccgtg gttcatggtg tcaagcatgg tacgaaacgc tggcggcgca ccggacattt
    16261 ccagaccgac atcaaagcct tcggtcatgc ccagttctgc catcacatca ttgaggttct
    16321 ctttagcgac gttaaccgca cgggtgatac ccattttacg cgccaactca aggcggtatt
    16381 cgttaacatc agtaatcacc acgtaacgtg caccaacgtg tttcgccacc gccgccgcca
    16441 taataccaat cgggcctgca ccagagacca gcacatcctc gcccaccaga tcgaacgaca
    16501 gcgcggtatg cacggcgtta ccgaagggat caaaaatgga agccagatca tcggagatat
    16561 tgtcggggat tttgaaagcg ttgaacgccg gaatcaccag atactcggcg aagcagcccg
    16621 gacggttaac gccgacgccg atcgtgttgc ggcacagatg ggtacgaccg ccacgacagt
    16681 tgcgacagtg accgcaggtg atatggcctt cgccagaaac gcgatcgcca atcttgaagc
    16741 ctttcacttc ctgaccaata cctaccactt caccgacata ttcatgaccc acgaccatcg
    16801 gcaccgggat ggttttttgc gaccactcat cccagttata gatgtgaacg tcagtcccgc
    16861 agatggctgt tttacggatt ttaatcagca gatcgttatg cccgagttcc ggtacaggaa
    16921 cgtcggtcat ccagatgccc tcttccgctt tcagtttgga taacgctttc atctcacatc
    16981 ctcaggcgat aacgcccagt tgtttaccaa tacgcgtgaa tgcttctact gcacgcgtaa
    17041 tttgctcagg ggtatgcgcc gcagacatct gggtacgaat acgcgcctga cctttcggaa
    17101 cgaccggata gaagaaaccg gtgacgtaaa tcccctcttt ttgcagctca cgggcaaatt
    17161 tctgcgccac taccgcatcg ccaagcatga ccggaataat ggcgtgatcg gctcccgcca
    17221 gggtaaagcc cgccgccgac atttgctcac ggaactgacg cgcgttcgcc cacagacggt
    17281 cacgcagctc gctacccgct tcaaccatct ccagcacttt gatggaagcg gcaacaatgg
    17341 ccggtgccag cgagttggag aacaggtacg gacgagaacg ctggcgcagc cactcaacca
    17401 cttctttgcg cgccgcggta taaccaccag aagccccgcc cagcgcttta ccaagcgtac
    17461 cggtgataat atcgacctgc cccatcacat cgcagtattc atgggaacca cgaccgtttt
    17521 caccgacaaa acctaccgcg tgtgagtcat ctaccatcac cagggcatca tatttatctg
    17581 ccagatcgca aacgcctttc aggttggcaa tcacgccgtc catcgagaac acaccatcgg
    17641 tggcaatcag cacatgacgc gcaccggctt cacgcgcttc tttcagacgt gcttccagct
    17701 cctgcatatc gttgttggca taacgatagc gtttagcttt gcacagacgc acaccatcaa
    17761 taatagacgc gtggttcagt gcgtcggaga taatggcgtc ttccgcgccc aacagcgttt
    17821 cgaacaggcc accgttagca tcaaagcagg aagagtagag aatcgcatct tccatcccca
    17881 ggaaggccgc cagtttttgt tcaagctctt tatggctgtc ctgagtgccg caaataaaac
    17941 gcaccgaagc catgccgaaa ccgtgagaat ccattcccgc ctttgccgcc gcaatcagat
    18001 ccggatgatt cgccagtccg agatagttat tggcacaaaa gttaatgacg tggcttccat
    18061 cagccacagt gatatctgct tgctgcgcag acgtaataat gcgctcttct ttaaacaacc
    18121 cttccgcccg tgcggtttcc agatcgttgg ttaactgctg ataaaattct ccacgcattg
    18181 cgattctcca gacagggcaa attccagcac atattaccca aacttatagg ttgcgacgag
    18241 atgacgcgtt aacacttctg caaaattcag gataaataac gtccagcccc ctcacttttg
    18301 gtgaatggct gaagccaacg tcatgtagtg atatgataga agtattcgtg tctgagattg
    18361 tctctgactc cataattcga aggttacagt tatgatcatc gttaccggcg gcgcgggctt
    18421 tatcggaagc aacatcgtta aagccctgaa tgataaaggc atcaccgata ttctggtggt
    18481 ggacaacctg aaagacggca ccaagtttgt gaacctggtg gatctggata tcgcggacta
    18541 tatggataag gaagacttcc tgatccagat tatggctggc gaagagttcg gcgatgtcga
    18601 agcgattttc cacgaaggcg cgtgctcttc caccaccgag tgggacggca agtatatgat
    18661 ggataacaac tatcaatact ccaaagagct gctgcactac tgtctggagc gcgaaatccc
    18721 gttcctgtat gcctcttccg cagccactta cggtggacgc acctccgact ttattgaatc
    18781 ccgcgaatat gaaaaaccgt tgaatgtcta cggttactca aaattcctgt ttgatgaata
    18841 tgttcgccaa atcctgcctg aagcgaactc acagattgtt ggcttccgtt atttcaacgt
    18901 ttatggaccg cgtgaaggcc ataaaggcag catggcgagc gtcgctttcc atctcaacac
    18961 ccagcttaat aacggtgaat caccgaagct gttcgaaggt agcgagaact tcaaacgtga
    19021 cttcgtctat gtgggcgatg tggcagatgt aaacctgtgg ttcctggaaa atggcgtttc
    19081 cggcatcttc aacctcggta ctggtcgtgc ggaatccttc caggcagtag cagatgctac
    19141 gcttgcttat cacaagaaag gccaaatcga atacattccg ttcccggata aactgaaagg
    19201 ccgctaccag gcgttcacgc aggcagatct gacaaatctg cgcgcggcgg gttacgacaa
    19261 accgtttaaa accgttgccg aaggcgtaac ggaatacatg gcctggctga atcgtgacgc
    19321 ataagagctc tgcatgaaaa tactggtgat cggcccgtct tgggttggcg acatgatgat
    19381 gtcgcaaagt ctctatcgca cgctccaggc gcgctatccc caggcgataa tcgacgtgat
    19441 ggcaccggca tggtgccgtc cattattatc gcggatgccg gaagttaacg aagctattcc
    19501 tatgcctctc ggtcacggag cgctggaaat cggcgaacgc cgcaaactgg gtcatagcct
    19561 gcgcgaaaaa cgttacgacc gcgcctacgt tttacccaac tccttcaaat ccgcattagt
    19621 gcctttcttc gcgggtattc ctcatcgcac cggctggcgc ggcgaaatgc gctacggttt
    19681 actcaacgat gtgcgtgtgc tggataaaga agcctggccg ctgatggtgg aacgctatgt
    19741 ggcgctggcc tatgacaaag gtattatgcg taccgcacaa gatctgccgc agccattgtt
    19801 atggccgcag ttgcaggtga gcgaaggtga aaaatcatat acctgtaatc aattttcgct
    19861 ttcatcagaa cgtccgatga ttggtttttg cccgggtgcg gagtttggtc cggcaaaacg
    19921 ctggccacac taccactatg cggagctggc aaagcaactg attgatgaag gttatcaggt
    19981 ggttctgttt ggctcggcga aagatcatga agcgggcaat gagattcttg ccgctttgaa
    20041 taccgagcag caggcatggt gtcgtaacct ggcgggggaa acacagcttg atcaagcggt
    20101 tatcctgatt gcagcctgta aagccattgt tactaacgat tctggcctga tgcacgttgc
    20161 ggcggcgctc aatcgtccgc tggttgccct gtatggtccg agtagcccgg acttcacacc
    20221 gccgctatcc cataaagcgc gcgtgatccg cctgattacc ggctatcaca aagtgcgtaa
    20281 aggtgacgct gcggagggtt atcaccagag cttgatcgac attactcccc agcgcgtact
    20341 ggaagaactc aacgcgctat tgttacaaga ggaagcctga cggatgcggg ttttgatcgt
    20401 taaaacatcg tcgatgggcg atgttctcca tacgttgccc gcactcactg atgcccagca
    20461 ggcaatccca gggattaagt ttgactgggt ggtggaagaa gggttcgcac agattccttc
    20521 ctggcacgcc gccgttgagc gagttattcc tgtggcaata cgtcgctggc gtaaagcctg
    20581 gttctcggcc cccataaaag ctgaacgcaa agcgtttcgt gaagcgctac aagcagagaa
    20641 ctatgacgca gttatcgacg ctcaggggct ggtaaaaagc gcggcgctgg tgacgcgtct
    20701 ggcgcatggc gtaaagcatg gcatggactg gcaaaccgct cgcgaacctt tagccagcct
    20761 gttttacaat cgtaagcatc atattgcaaa acagcagcac gccgtagaac gcacccgcga
    20821 actgtttgcc aaaagtctgg gctacagcaa accgcaaacc cagggcgatt atgctatcgc
    20881 acagcatttc ctgacgaacc tgcctacaga tgctggcgaa tatgccgtat ttcttcatgc
    20941 gacgacccgt gatgataaac actggccgga agaacactgg cgagaattga ttggtttact
    21001 ggctgattca ggaatacgga ttaaacttcc gtggggcgcg ccgcatgagg aagaacgggc
    21061 gaaacgactg gcggaaggat ttgcttatgt tgaagtattg ccgaagatga gtctggaagg
    21121 cgttgcccgc gtgctggccg gggctaaatt tgtagtgtcg gtggatacgg ggttaagcca
    21181 tttaacggcg gcactggata gacccaatat cacggtttat ggacctaccg atccgggatt
    21241 aattggtggg tatgggaaga atcagatggt ttgtagggct ccggggaatg agttgtctca
    21301 attgacagca aatgctgtta agcaattcat tgaagaaaac gctgaaaaag ctgccatgat
    21361 ttaaattaca actctttaat
//
LOCUS       DAZGQL010000086        21188 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.086, whole genome shotgun sequence.
ACCESSION   DAZGQL010000086 DAZGQL010000000
VERSION     DAZGQL010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21188
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.086"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..299
                     /locus_tag="ACHKIR_003743"
     CDS             <1..299
                     /locus_tag="ACHKIR_003743"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HFO9991422.1"
                     /translation="DRHQDHLAVYQASMVACRAIPQILGYETPSTWLSFMPQVFESVK
                     EEYFSLKLTALKKHKSQSQRDYMRPERLRAVAQFRGQQVNSDLGEGFVIHKMIL"
     gene            309..1505
                     /gene="yaiP"
                     /locus_tag="ACHKIR_003744"
     CDS             309..1505
                     /gene="yaiP"
                     /locus_tag="ACHKIR_003744"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFO9991423.1"
                     /translation="MKTWIFICMAVAILLWFLSTLRRKPSQKKGCIDAIIPAYNEGPC
                     LAQSLDNLLRNPYFCRVICVNDGSTDNTEAVMAEVKRKWGDRFIAVTQKNTGKGGALM
                     NGLNYATCDQVFLSDADTYVPPDQDGMGYMLAEIERGADAVGGIPSTALKGAGLLPHI
                     RATVKLPMIVMKRTLQQLLGGAPFIISGACGMFRTDVLRKFGFSDRTKVEDLDLTWTL
                     VANGYRIRQANRCIVYPQECNSPREEWRRWRRWIVGYAVCMRLHKRLLFSRFGIFSIF
                     PMLLVVLYGVGIYLTTWFNEFITTGPHGVVLAMFPLIWVGVVCVIGAFSAWFHRCWLL
                     VPLAPLSVVYVLLAYAIWIIYGLIAFFTGREPQRDKPTRYSALVEASTAYSHPSVTGT
                     EKLSEA"
     gene            1540..2064
                     /locus_tag="ACHKIR_003745"
     CDS             1540..2064
                     /locus_tag="ACHKIR_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFO9991424.1"
                     /translation="MIIDESAGEVVIGANTRICHGAVIQGPVVIGANCLIGNYAFIRP
                     GTIISNGVKIGFATEIKNAVIEAEATIGPQCFIADSVVANQAYLGAQVRTSNHRLDEQ
                     PVSVRTPEGIIATGCDKLGCYIGQRSRLGVQVIILPGRIISPNTQLGPRVIVERNLPT
                     GTYSLRQELIRTGD"
     gene            2066..2839
                     /gene="yaiO"
                     /locus_tag="ACHKIR_003746"
     CDS             2066..2839
                     /gene="yaiO"
                     /locus_tag="ACHKIR_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HFO9991425.1"
                     /translation="MIKRTLLAAAIFSTLPAYAGLTSITAGYDFTDYSGDHGNRNLAY
                     AELVAKVENATLLFNLSQGRRDYETEHFNATRGQGAVWYKWNNWLTTRTGIAFADNTP
                     VFARQDFRQDINLALLPKTLFTTGYRYTKYYDDVEVDAWQGGVSLYTGPVITSYRYTH
                     YDSSDAGGSYSNMISVRLNDPRGTGYTQLWLSRGTGAYTYDWTPETRYGSMKSVSLQR
                     IQPLTEQLNLGLTAGKVWYDTPTDDYNGLQLAAHLTWKF"
     gene            3024..3299
                     /gene="frmR"
                     /locus_tag="ACHKIR_003747"
     CDS             3024..3299
                     /gene="frmR"
                     /locus_tag="ACHKIR_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HFO9991426.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            3334..4443
                     /gene="frmA"
                     /locus_tag="ACHKIR_003748"
     CDS             3334..4443
                     /gene="frmA"
                     /locus_tag="ACHKIR_003748"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HFO9991427.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            4535..5368
                     /gene="fghA"
                     /locus_tag="ACHKIR_003749"
     CDS             4535..5368
                     /gene="fghA"
                     /locus_tag="ACHKIR_003749"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046294 - formaldehyde catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFO9991428.1"
                     /translation="MELIEKHASFGGWQNVYRHYSPSLKCEMNVGVYLPPKAANEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMQHFPATTRKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFAPIVSPSQVPWGQQAFTAYLGENKDAWLDYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(5595..6134)
                     /gene="yaiL"
                     /locus_tag="ACHKIR_003750"
     CDS             complement(5595..6134)
                     /gene="yaiL"
                     /locus_tag="ACHKIR_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HFO9991429.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLVDNNSEGEYAIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDYLMW"
     gene            complement(6236..7447)
                     /gene="mhpT"
                     /locus_tag="ACHKIR_003751"
     CDS             complement(6236..7447)
                     /gene="mhpT"
                     /locus_tag="ACHKIR_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HFO9991430.1"
                     /translation="MSTRTPSSSSTRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGEKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALAPLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGIGTVGVMAASAPGILVAGLAVFILMS
                     RRSRMQPCADA"
     gene            complement(7623..8636)
                     /gene="mhpE"
                     /locus_tag="ACHKIR_003752"
     CDS             complement(7623..8636)
                     /gene="mhpE"
                     /locus_tag="ACHKIR_003752"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HFO9991431.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQVAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVKHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAVLKPETQTGIHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAADKLGWQHGTDLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            complement(8633..9583)
                     /gene="mhpF"
                     /locus_tag="ACHKIR_003753"
     CDS             complement(8633..9583)
                     /gene="mhpF"
                     /locus_tag="ACHKIR_003753"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HFO9991432.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHGQHLEMAVMVGIDPQSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEENVAQLNVNMVTCGGQATIPMVAAVSRVARVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            complement(9580..10389)
                     /gene="mhpD"
                     /locus_tag="ACHKIR_003754"
     CDS             complement(9580..10389)
                     /gene="mhpD"
                     /locus_tag="ACHKIR_003754"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HFO9991433.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDNAEAAYAIQHI
                     NVQHDVAQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNETIPFSRVLQP
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAATFSSAAPKGSLS"
     gene            complement(10399..11265)
                     /gene="mhpC"
                     /locus_tag="ACHKIR_003755"
     CDS             complement(10399..11265)
                     /gene="mhpC"
                     /locus_tag="ACHKIR_003755"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414883.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HFO9991434.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVKAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHSSVAFTLNWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQ
                     LYRQPTIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLEANPKQFP
                     DFGPRLAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADA
                     FNQLVLNFLARP"
     gene            complement(11283..12227)
                     /gene="mhpB"
                     /locus_tag="ACHKIR_003756"
     CDS             complement(11283..12227)
                     /gene="mhpB"
                     /locus_tag="ACHKIR_003756"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydroxyphenylpropionate/2,
                     3-dihydroxicinnamic acid 1,2-dioxygenase"
                     /protein_id="HFO9991435.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISAFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            complement(12229..13893)
                     /gene="mhpA"
                     /locus_tag="ACHKIR_003757"
     CDS             complement(12229..13893)
                     /gene="mhpA"
                     /locus_tag="ACHKIR_003757"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008688 - 3-(3-hydroxyphenyl)propionate
                     hydroxylase activity [Evidence IEA];
                     GO_process: GO:0019622 - 3-(3-hydroxy)phenylpropionate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HFO9991436.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVDNVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGLSRFPNVRCLFSRELEAFSQQDDEVTLHLKTAEG
                     QRETVKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDAL
                     LDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALGTRFIQVVPEVQIHTAQDNHDGVLRVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGNTLNKLASVMTLTRPDADVSVEKVA"
     gene            14084..14917
                     /gene="mhpR"
                     /locus_tag="ACHKIR_003758"
     CDS             14084..14917
                     /gene="mhpR"
                     /locus_tag="ACHKIR_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HFO9991437.1"
                     /translation="MQNNEQTAYKTVRGLTRGLMLLNMLNKLDGGASVGLLAELSGLH
                     RTTVRRLLETLQEEGYVRRSPSDDSFRLTIKVRQLSEGFRDEQWISALAAPLLGDLLR
                     EVVWPTDVSTLDVDAMVVRETTHRFSRLSFHRAMVGRRLPLLKTASGLTWLAFCPEQE
                     RKELIEMLASRPGDDYQLAREPLKLEAILARARKEGYGQNYRGWDQEEKIASIAVPLR
                     SEQRVIGCLNLVYMASAMTIEQAAEKHLPALQRVAKQIEEGVESQAILVAGRRSGVHL
                     R"
     gene            14994..16076
                     /gene="lacI"
                     /locus_tag="ACHKIR_003759"
     CDS             14994..16076
                     /gene="lacI"
                     /locus_tag="ACHKIR_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HFO9991438.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQACHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACANVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLPPNT
                     QTASPQALADSLMQLARQISRLESGQ"
     gene            16199..19273
                     /gene="lacZ"
                     /locus_tag="ACHKIR_003760"
     CDS             16199..19273
                     /gene="lacZ"
                     /locus_tag="ACHKIR_003760"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HFO9991439.1"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSKEAR
                     TDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPDADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPAENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDISLLHK
                     PTTQISDFHVTTRFNDDFSRAVLEAEVQVYGELRDELRVTVSLWQGETQVASGTTPFG
                     GEIIDERGGYADRVTLRLNVENPALWSAEIPNIYRAVVELHTADGTLIEAEACDVGFR
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVLRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTSATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGELRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQ
                     QQFFQFRLSGRTIEVTSEYLFRHSDNELLHWSVALDGKPLASGEMPLDVAPQDKQLIE
                     LPELPQPESAGQLWLTVHVVQPNATAWSEAGHISAWQQWRLTENLSVTLPSAPHAIPQ
                     LTTSETDFCIELGNKRWQFNRQSGFLSQMWIGDEKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQTEVALLQCTADTLADAVLITTAHAWQHQGKTLFISRKT
                     YRIDGSGQMAITVDVEVASDTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
     gene            19325..20578
                     /gene="lacY"
                     /locus_tag="ACHKIR_003761"
     CDS             19325..20578
                     /gene="lacY"
                     /locus_tag="ACHKIR_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactose permease"
                     /protein_id="HFO9991440.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYN
                     ILVGSIVGGIYLGFCFNAGAPAVEAFIEKVSRRSNFEFGRARMFGCVGWALCASIVGI
                     MFTINNQFVFWLGSGCALILAVLLFFAKTDAPSSATVANAVGANHSAFSLKLALELFR
                     QPKLWFLSLYVIGVSCTYDVFDQQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIM
                     FFAPLIINRIGGKNALLLAGTIMSVRIIGSSFATSALEVVILKTLHMFEVPFLLVGCF
                     KYITSQFEVRFSATIYLVCFCFFKQLAMIFMSVLAGNMYESIGFQGAYLVLGLVALGF
                     TLISVFTLSGPGPLSLLRRQVNEVA"
     gene            20644..>21188
                     /gene="lacA"
                     /locus_tag="ACHKIR_003762"
     CDS             20644..>21188
                     /gene="lacA"
                     /locus_tag="ACHKIR_003762"
                     /EC_number="2.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactoside O-acetyltransferase"
                     /protein_id="HFO9991441.1"
                     /translation="MNMPMTERIKAGKLFTDMCEGLPEKRLRGKTLMYEFNHSHPSEV
                     EKRESLIKEMFATVGENAWVEPPVHFSYGSNIHIGRNFYANFNLTIVDDYTVTIGDNV
                     LIAPNVTLSVTGHPVHHELRKNGEMYSFPITIGNNVWIGSHVVINPGVTIGDNSVIGA
                     GSVVIKDIPPNVVAAGVPCRV"
BASE COUNT         4927 a         5756 c         5522 g         4983 t
ORIGIN      
        1 ctgaccgcca tcaggatcat cttgcagtct atcaagcctc aatggttgcc tgtcgcgcta
       61 ttccgcaaat tctcggctat gaaacaccaa gtacctggct ttcatttatg cctcaggttt
      121 ttgaatccgt gaaagaagaa tatttttctc ttaaactgac tgcgttaaaa aaacataaaa
      181 gtcagagcca gcgagattat atgcgcccgg agcgtctgcg tgcagttgct caatttcgcg
      241 ggcaacaggt caatagcgat ctgggtgaag gttttgttat tcataaaatg attctttgag
      301 caaattgcat gaaaacctgg atctttatat gtatggccgt agcaatattg ctatggtttc
      361 tgagtacgtt aaggcgtaag cccagccaaa agaaaggctg tattgacgcc attatacctg
      421 cttataacga aggcccgtgt ctggcgcagt cactggataa tctgctgcgt aacccttatt
      481 tctgccgggt aatttgcgtt aacgacggct ccacggacaa taccgaagcg gtcatggcgg
      541 aagtcaaacg caaatggggc gaccgcttta ttgccgtcac gcaaaaaaat accggtaaag
      601 gtggtgcgct aatgaatggc ctcaattacg ccacctgcga tcaggttttt ttaagtgatg
      661 ccgacaccta tgttccgccc gatcaagacg gaatgggcta tatgctggca gaaatagagc
      721 gcggtgctga tgccgtaggc ggcattccct ctactgcgtt gaaaggcgcg ggtctgttac
      781 cgcacatccg cgcgaccgta aagttgccga tgattgttat gaagcgcacg ctacagcagc
      841 tcctgggcgg cgcaccgttt attatcagcg gtgcctgcgg gatgttccgt actgatgtat
      901 tgcgtaagtt cggtttctct gatcgtacta aagtcgaaga ccttgatctc acctggacat
      961 tggtggcaaa cggctatcgt attcggcagg cgaatcgctg catcgtatac ccacaggaat
     1021 gcaacagccc gcgtgaggaa tggcgtcgct ggcggcgttg gattgtgggc tacgcggtct
     1081 gtatgcgcct gcataaaaga cttttattta gccgcttcgg tatcttcagt atatttccta
     1141 tgctgttggt tgtgctttat ggcgttggga tttatctcac tacctggttt aatgaattca
     1201 tcaccaccgg gccacatggt gtggtgttgg caatgttccc gcttatctgg gtcggcgtag
     1261 tttgtgttat tggtgcattt agcgcctggt ttcatcgttg ctggttgttg gtgcctttag
     1321 caccgctttc cgttgtgtat gtattattag cttatgccat ctggattatt tatggactta
     1381 ttgccttttt tactggacgc gaacctcagc gcgacaaacc cacccgctat tccgcactgg
     1441 tggaagcgtc aaccgcttat tcccaccctt ctgtcacagg aactgaaaaa ctatctgaag
     1501 cttaatgtta aagagaaaaa tattcagatt gcagaccagg tgattattga tgaaagtgcc
     1561 ggtgaggttg ttatcggcgc gaatacccgt atttgccacg gtgccgttat tcagggtccg
     1621 gtagtgattg gcgcaaactg cctgataggt aattatgcgt ttattcgtcc tggcacaata
     1681 atcagcaatg gcgtaaaaat tggttttgcc accgaaatta aaaatgcggt tattgaagcg
     1741 gaagcaacga ttggtccgca atgctttatt gccgactcgg tagttgcaaa ccaggcatat
     1801 ttgggcgcac aagtacgaac cagtaatcat cgtctggatg aacagcccgt gtctgttcga
     1861 actccagagg gaattatcgc taccggatgc gataaattag gttgttatat cgggcagcgt
     1921 tcacgccttg gcgtacaagt tattattttg cctgggcgaa ttatttcccc gaacacgcaa
     1981 cttggcccgc gcgtgattgt agaacgtaat ttacctaccg gaacttattc actccgacaa
     2041 gaacttatcc gtacaggaga ttaaaatgat aaaacggacg ttattagcgg cggcaatatt
     2101 tagcacattg cccgcttatg ccgggttaac ttccattacc gcgggctacg attttaccga
     2161 ttattctggc gatcatggca accgtaattt agcgtatgct gaactggtgg cgaaagttga
     2221 aaacgcaacg ctgcttttta atctttccca ggggcgtcgt gattatgaaa ctgaacattt
     2281 caatgccact cgcggtcagg gtgcggtctg gtataaatgg aataactggc tgacaacccg
     2341 aacgggtatt gcctttgcgg ataatacgcc ggtctttgcc cgccaggatt ttcgtcagga
     2401 tattaacctg gccctgttgc caaaaacgct tttcacgacc ggttatcgct acactaaata
     2461 ttacgatgat gtcgaagtcg atgcctggca aggcggcgta tccctctata caggcccggt
     2521 gatcaccagc tatcgctata cccattatga ctccagcgat gcaggtggta gttacagcaa
     2581 tatgatttcc gtgcgtctga atgacccgcg cggcactggt tatacgcaac tatggctaag
     2641 ccgcggaaca ggtgcttaca cctatgactg gacgccagaa acacgctacg gcagcatgaa
     2701 gagcgtcagt ttgcaacgta ttcaaccgct gactgagcaa cttaatctcg ggctgacggc
     2761 aggtaaagtg tggtacgaca ccccaaccga tgattataac ggtctgcaac ttgcagccca
     2821 tctgacctgg aaattctgat tccttctgcc gcccgctatc cggggcggcc ttccctgtcg
     2881 attaggcccc tctttacttt ttgttatccg actacattca ccggataaat tttattctcc
     2941 agtgttatat actatagagg ggtatgcatt gacatataac atacccccct atagtatatt
     3001 gcgtgcagat gatgaggtgc gaaatgccca gtactccgga agagaagaaa aaggtcctta
     3061 ctcgagttcg tcgtattcgg gggcagattg atgctctgga acggtcgctg gagggtgatg
     3121 ccgaatgccg tgccatactc caacagatcg ctgccgttcg gggcgcggct aacggactga
     3181 tggcagaagt gcttgaaagc catatccggg aaacgtttga ccgaaatgac tgctacagcc
     3241 gcgaagtcag ccaatccgtt gacgacacta ttgaactggt tcgtgcctat cttaaatagc
     3301 tgaatctatt accatattga ggaagagcga gagatgaaat cacgtgctgc cgttgcattt
     3361 gctcccggta aaccgctgga aatcgttgaa attgacgttg caccaccgaa aaaaggtgaa
     3421 gtgctgatta aagtcaccca taccggtgtt tgccataccg acgcatttac cctctccggc
     3481 gatgacccgg aaggtgtctt cccggtggtt ctcggtcacg aaggggccgg ggttgtagtt
     3541 gaagtcggtg aaggcgtaac cagcgtcaaa cctggcgacc atgtgatccc gctttacacc
     3601 gcagagtgcg gcgagtgtga gttctgtcgt tctggcaaaa ccaacctgtg tgtcgcggtt
     3661 cgcgaaacgc agggcaaagg cttgatgcca gacggcacca cccgtttttc ttacaacggg
     3721 cagccgcttt atcactacat gggatgctca acattcagtg aatacaccgt ggtcgcggaa
     3781 gtgtctctgg ccaaaattaa tccagaagca aaccatgaac acgtctgcct gctgggctgt
     3841 ggcgtgacca ccggtattgg cgcggtacac aacacagcta aagtccagcc aggtgattct
     3901 gttgccgtgt ttggtcttgg cgcgattggt ctggcagtgg ttcagggcgc gcgtcaggcg
     3961 aaagcgggac ggattatcgc tatcgatacc aacccgaaga aattcgatct ggcgcgtcgc
     4021 ttcggtgcca ccgactgcat taacccgaat gactacgaca aaccgattaa agatgtcctg
     4081 ctggatatca acaaatgggg tatcgaccat acctttgaat gcatcggtaa cgtcaacgtg
     4141 atgcgtgcgg cgctggaaag tgcgcaccgc ggctggggtc agtcggtgat catcggggta
     4201 gctggttctg gtcaggaaat ctccacccgt ccattccagt tggtcaccgg tcgcgtatgg
     4261 aaaggttccg cgtttggcgg cgtgaaaggt cgttcccagt taccgggtat ggttgaagat
     4321 gcgatgaaag gcgatatcga tctggaaccg tttgtcacgc ataccatgag cctggatgaa
     4381 attaatgacg cctttgacct gatgcatgaa ggcaaatcca ttcgaaccgt aattcgttac
     4441 tgatttcccg caggtttacc cgtccagttc agacggggtt cttaatactc tccccgggca
     4501 gccgtccggg ggattaaccg tgagataatg actgatggaa ctcattgaaa aacatgccag
     4561 ctttggcggc tggcaaaatg tgtatcggca ttattcccca tcactgaaat gtgaaatgaa
     4621 tgtcggcgtc tatctcccgc caaaagccgc aaatgaaaaa ttgccggtgt tgtactggct
     4681 ttcaggcctg acctgcaacg agcagaattt cattactaaa tcggggatgc agcgttacgc
     4741 ggctgagcac aacattattg ttgttgcgcc agacaccagt ccgcgaggca gtcatgtcgc
     4801 agatgccgac cgttacgatc tcgggcaagg tgccgggttt tacctgaacg caacgcaagc
     4861 gccgtggaat gaacattaca aaatgtatga ctatatccgc aacgagctgc cggatttagt
     4921 gatgcaacat tttccggcaa cgaccagaaa gtctatctca ggtcattcta tgggcgggct
     4981 gggcgcgctg gtgctggcgt tacgtaaccc agatgaatat gtcagcgtct cggcgtttgc
     5041 gcccattgtc tccccatcgc aagtaccgtg gggacagcaa gcctttaccg catatcttgg
     5101 tgaaaataaa gatgcctggt tggattacga tccggtgagt cttatttcac aaggtcaacg
     5161 cgttgcggaa ataatggttg atcaggggtt gagtgatgat ttttacgcag aacagctgcg
     5221 gactccaaat cttgaaaaga tctgccagga gatgaatatc aaaacgttaa tccgttatca
     5281 cgagggttat gatcacagct attattttgt ctccagtttt attggcgagc atattgcgta
     5341 ccacgccaat aaactgaata tgcgttgagc atccgcgcac gcatgccgga tgcggcgtaa
     5401 acgccttatc cggcctacat ttcacccgta agccgtaggc ctgataagac gcgaaaagcg
     5461 tcgcatcagg catctgcgca cggctgccgg atgcggcgtg aacgccttat ccggcctaca
     5521 cttcgcccgt aaaccgtagg cctgataaga cgcgtaagcg tcgcatcagg catcactgca
     5581 cggcttatct atgtttacca catcaaataa tcaggcactt tgaagtcggc atacggatcg
     5641 tcttcatcct gctcttcggc gctgagcgcg ctgtgcaaca caatactgct ggcatcacgc
     5701 tgagcaattt tatcggcaac actggcggga ataatggcgt attcaccttc gctattgtta
     5761 tcgaccaaca agcgggcaat cgccagacga ccattaatca actgcgcctg tgtgagctta
     5821 tcgacaaaaa tcttcttaat cagattgccg tcagtgaagt taaaaccaat atcgccattg
     5881 gcaatggtga ttcggttcat ttcaatgagc tgcttcacct gggctttata ttctttcgcc
     5941 aacgccgctt gtttttgctg ttcgctaagc tgtttatcac gctcaagctg tgccttttta
     6001 ttttcttcta ccgccgcccg agcttcacgc gcctgaacgc gcgatttttt cgccgttctc
     6061 tcgaccttcg ccgctttttt gctggtgact aatcctgctt tgagcaactg ctcttgtaag
     6121 gtaagttttg ccatctttgt ttccaaaccc gctgaataat ttttagcgag tatacctgta
     6181 aacgccgtag gtgtattggg ggcaagtccc tttcgcccct gcttaataag gcgcgtcagg
     6241 catcggcgca cggctgcatt cgtgatctcc ggctcatcaa aataaatact gccaggccag
     6301 caacaagaat acccggcgca gaagcggcca ttacgccaac cgtgccaatg cctaatgcca
     6361 gcattttccc ggccagtaac ggaccgctca tagcccccag acgccctacc gccacggctg
     6421 ttcccacacc tgttgcgcgg atctgcgaac tgtaaaacaa cggtgccagg gcatacaaaa
     6481 cgctttgccc acctgtcgca aacaaccccg cgacgaatcc cgccagcaac ataccgttaa
     6541 atgacgacac cgttcccagc gccagcagcg aagctaacat gccgctataa atcagtagcg
     6601 acatggttac tggacgcagc ttatccatca atgcgcccaa cattaacgtc ccgcttgccg
     6661 cccccatttg cagtgcaaac atcacccctg ccgcctgcga tggctggaat ccttgctcca
     6721 ccaaaagcag cggtagccag ttgatcaaca tgtagaccac cagcagagtg aagaaataac
     6781 acaaccacag cagcagcgtc gcggttgccg tttctggcgc aaataacgca cgcagtggtg
     6841 gcgcagcctg tttttcgcca gcgaacaccg ccgactccgg cagccagcgc attaatagcg
     6901 gcaccagaat caacggcacc acaccaccta cccaaaacac cgtttgccat gctaagtttg
     6961 cccccgcgaa acccagtgtc gccgccagcg ccgcgccaat ggggacaccg caatacatca
     7021 ggctcactgc cgtcccacga aaacgtggac ccgcggcttc agacgtcagg gcgatcagat
     7081 tcggcaacgc cgcccccagc ccgacaccgg tcatcagccg cgcaaagacc agtgagggga
     7141 aatcccaggc aatcgccgtt gccagtgaga acaaaccaaa cagcgcaact gagccaatca
     7201 aaatgcgctt gcgaccataa cggtccgcca gcattccgcc aaccaacgcg ccgggtagca
     7261 aaccgagtat tccggcgctg aatatccagc ccatttgcat tttatcgagt gcgaaagcct
     7321 gggcgatgcc acccgccgca atgccagccg cctgaagatc cagtccttcc atcagagcga
     7381 ccaaaaaaca aagcccgatg gtcagcatca ggcgggttga agatgatgaa ggggtacgag
     7441 tcgacataag atgaacctcg ttgcgagtgc aggtgaatcg cggctgatac attgtcagcc
     7501 gcgtaatcag aaccatttaa tgactgccta cgggcgaaat ataggccgga taaggcgttt
     7561 acgccgcatc cggcagtcgt gctgggatgg gcatacaaaa tgcacgttct cggcagtcat
     7621 cattatttgt tgttgcgcag atccagcgcc acgtcgacga tcatatcctc ctggccgcca
     7681 accatccggc gtttgcccag ctcaacgaga atatccaccg cgcttaagcc ataacgcgcc
     7741 gccgccgttt cacagtgacg caggaagctc gagtaaacac cagcgtatcc cagcgccagc
     7801 gtttcgcgat cgactcgtac cggtcgatcc tgcaacggac gcaccaggtc gtcagcggca
     7861 tccattaacg catagagatc ggtcccatgc tgccaaccca gtttatccgc ggcggcaata
     7921 aaaacttcca gcggcgcgtt acctgcgccc gcgcccattc ccgcgaggct ggcgtcgatt
     7981 cggtcgcagc cctcttccac cgccgcgata gagttcgcca cgccaagact caggttatgg
     8041 tgagcgtgta tgccggtttg cgtttctggt ttcagcactg ctttcagagc gcggaaacgg
     8101 tcacggatat cgctcatgtt catcgcaccg ccagaatcca ccacataaat acaggtcgca
     8161 ccgtagcctt ccatcagctt tgcctgcttt gcgagattct ccggcgtggt catatggctc
     8221 atcatcagaa aaccaacggt gtccattccg agctcgcggg catactgaat atgctgggcg
     8281 gaaacatcag cttcggtaca gtgcgtagcc acacgaacca cccgcgcgcc agcctgccag
     8341 gcatttttca gatcgtgaat agtgccgatt ccaggcagca acaacgtcgc gattttggcg
     8401 tgcttcacca catccgccgc cgcttcaatc cattcaaggt cgctatgtgc gccgaaacca
     8461 tagttaaagc tggaaccttg caaaccgtcg ccgtgggcca cttcaatcga atccacgcgg
     8521 gcatcgtcca gtgctttggc aacctggcga acgttttcca atgaatactg atgacgaatg
     8581 gcgtgcatac cgtcacgcaa cgtgacgtcc gagatataaa gttttttacc gttcatgccg
     8641 cttctcctgc cttgcgcgcc agtgactggg ccattttttc cgctgtcgcc agcgcactgg
     8701 aagtcataat gtcgaggttg cctgcatagg caggcagata atgcgctgcg ccttcgactt
     8761 ccagccagac cgccgttttc agtccggaga actgccccac gcccggcaaa ttaaccggtt
     8821 tatcctgcgg gataacttca aactgcacgc gctgtttcag gcgataaccc ggtacgtaag
     8881 cctgcaccgc ctcagccatt tcattgattg aggcttcgat atcatcttgt gaagcttcgt
     8941 cgctcaatac atacaccgtg tcacgcatca tcaacggtgg ctctgctggg ttaagcacaa
     9001 taatcgcctt cccttttgcc gcgccaccca ccacttcaat ggcgcgcgaa gtggtttccg
     9061 taaattcatc gatattggca cgcgtgccag gtccggcaga tttactggca atagaagcga
     9121 taatttcggc gtaatgaaca cgcgccacgc gtgaaactgc cgccaccatt ggaatggtgg
     9181 cctggccgcc gcaggtgacc atgttgacgt tcagttgagc gacgttctcc tcgaggttaa
     9241 cgaccggcac gcagtagggg ccgatggcag caggcgtcag gtcaattaag cgaatatccg
     9301 gtttcgcttc gcgcaaagcg gcatcgtttt tcacatgcgc acccgcgctg gtcgcatcaa
     9361 atacaatgtc gatatcagca aattcaggca tgttcatcag gccgatcacc ccttcatggg
     9421 tggtggcgac gcccatacgt ctggcacgcg ccagaccgtc ggactgagga tcaatgccga
     9481 ccatcaccgc catctccaga tgctggccgt gacgcaaaat tttaatcatc agatcggtgc
     9541 caatgttgcc tgaaccgata atggcgactt tacgcttact catgacagac ttccttttgg
     9601 ggctgcgctt gaaaatgtcg cagcaactga acctatgcct tcaatatggg cttcaaaacg
     9661 atcgcccgca ttcaccgcca ccatcggacc taatgccccg gtaagaatga tatctccggc
     9721 gcgcagcggt tcacccaggc tggccatttt gcgtgccagc cagacggcgg cattaagcgg
     9781 atgtcccagg cattcgctgc cgcgcccgct ggaaacctct tcgttattac gcgtcatctt
     9841 catggcgcag tttttcaggt ctaaccccgc cggacgttgc gccggaccgc cgatgacata
     9901 caccccgcag gaagcgttat ctgccacggt atcgacaaac tgaatcgacc agtcgcgaat
     9961 gcggctcccc accacttcca gcgccggaag tacccattca atggcgttat acaattcgtc
    10021 gaaggtgata tcggttgcgg gtagatcgcg gttcaacacc agtgcaatct ccgcttcaat
    10081 gcggggttgc agaacacggg aaaaaggaat ggtttcgtta tcgccataac acatgtcggc
    10141 aaataatgtc ccgaaatccg gttgatcaac gcccagttgt tgctgcactt tcggatgcgt
    10201 cagccccact ttgcgcccca ccactcgccg tccctgagcg acgtcgtgtt gcacattgat
    10261 gtgctgaatg gcgtaggccg cttcagcgtt atcgatacca atcagatcgc gcagcggcgc
    10321 aatcgcttcg ccctgctctg cggcgcgacg taaatccgcc gccagttgct caagagtatg
    10381 cttcgtcatg accgttcctt aagggcgtgc gaggaaattc agcaccagtt gattgaaagc
    10441 gtcggcatgt tcccactgcg cccagtgacc acagtcgcgg aagatatgca gttcagaacc
    10501 ggcaatgccg gacagcagac gcagacccgc atccatcggc acaaagcggt cgttgcgccc
    10561 ccagacaatc agggtttgcg ctttgatttc cgccagacgt gggccaaaat ccgggaactg
    10621 tttcggattg gcttccaggc tcttaacgaa gttttccagg tgatcgcggc gcgacagcat
    10681 attattcagg cgcgcttcaa acagggcgtc ggtcaaatcg ctggtatcaa aaacgaagat
    10741 atccatcatc agcttcaggt tttcgatagt cggctgacga taaagctgat tcagtcgctt
    10801 aataccttcg gttggcatcg gcgtaaacaa actcatgccg cccgtaccgc cgcccatcag
    10861 caccagtttg ccgacgcgct ccggccagtt aagggtgaac gccacagaac tgtggccgcc
    10921 catcgagttg cccagcaggt ggattttggc gatatccagt tgatccacca cgcttttcag
    10981 gattcgcgca ttaagatccg atcgtgaacc actattaacg atcgaatcgc tcttacccca
    11041 acccggacaa tccagcagga tcacccgata gcccgccttt accagcggat cgatattgcg
    11101 gctgaagttc gcccagccag tagcacccgg gccggaacca tgcagcagga caacagtttc
    11161 gtcgccttgt ccgcagtcat taaaatggat gcgcagcgtt ttacccgctt cttctacatt
    11221 cagaaaacgg ctggtggcgg cttcggtttg tggctgataa ctcatcatct tctcctgcat
    11281 attcagttct ctgttctggc gcttaacgag ccaaatccgg caatccactc cgggattggg
    11341 cggtaataac gcccttcgct acgccagttg ccaaacgcag aaatagcggc aaaagcggcg
    11401 acccaggttt tgatttcatg tgtcgacttt ccggcaatgg cggaaagctc ttcgttactg
    11461 acggcatcca gttcctgtat gcgtccctgc tccagcaaag tcatgaactg gttatcccaa
    11521 atcgggttga gcggatgcag cgttctctga tcctcaacaa acttctcagc ggcgctaatc
    11581 acccgttgct gacgcaattc gcgctcactg gcgggtaaat ctttcccgct ccccaacaga
    11641 cggtcgcgca tatgggcatc ggctttcgcc agttctggca ccggcggctg atgggaaagc
    11701 ccaccggaac ccaggaacag cacgcgttta ttgagagtac tggtgaaacg tccaatggct
    11761 tcccccaaca tgcgggtacg ctggaaaccg ggcaacggcg tggcgacacc gttgatgaac
    11821 acaggcagaa ccggcacctt atccagccca ccgagcagga actccagcgg ctgggcgaac
    11881 ccgtggtcca cctgcataca gtaagaaacg gcaagatcga ttccgctctt catgacggca
    11941 tgcgcacagg cctccgccag ctcaacaggc acgggcagct ctcctgccgc actaccgaaa
    12001 tcaccaattg ccgtcgctcc aacgcctaaa cagaacggtg gcatcacgtc atagaaaaag
    12061 ccgttgtagt gatctggcgc aaacagcacc accaactcag gggagaaagc cgcaatacgc
    12121 tcgcgggcgc tggcaatcac gccattgacc tcatcgagca cctcttgcgc cgggtcgaca
    12181 tatcccacca gcggcgagtg ggaaagacag tgaagataag cgtgcatatc aggctacctt
    12241 ttcgacagaa acgtcggcat cagggcgggt cagcgtcatt accgacgcca gtttattcag
    12301 cgtattaccc agagtttgcg gaatggcggt ggcggcaaca aagcgatccg ggcgcatcac
    12361 caccagcgaa gcattgtgtt gcgcgaacca gctacgcagg cgaccttgcg tatcgcccac
    12421 gcgtagtacg ccgtcgtggt tatcctgtgc ggtatgaatt tgcacttccg gcaccacctg
    12481 aatgaagcgt gtgcccaacg cgcgccactg ctggatttgc tcgtcgctca tcccccacag
    12541 tggattgcat ccccagccaa ttaccgcgaa gttcgcgccg atcgcgttat cgagcagcgt
    12601 cacgtcgccg ttttccagcg tgactttcgg ctgaataaac atcttgccga ccggagagtg
    12661 cttcgcttcg ccctcacgca ccagcgcacc gccgtaatat tgcggcatcg gcttgaagcg
    12721 catttcgagg aagtagcgtt ttactggcgg cagataattc aacagccagg aaacgccgtc
    12781 acgtaacgta ccctgccagc gtttcggcgg agccagcacg ttgcccgccg tcacggacag
    12841 gtcaatcatc gctttggcgt gatcgcgacg ttcttgttga taggtatcga gcagcgcatc
    12901 gcgggctttc ccctggataa ccaacgccag tttccatgcg aggttaaagg cgtcgcgcat
    12961 accactgtta tagccctgcc cctgccacac cggcatgatg tgcgcggcat cgcccgccag
    13021 cagtacgcga tcaatacgaa aacgttgcgc cagtcgcgcg ttgtgggtgt agacacgctg
    13081 gcgaatcaat tcaacattgt ccggattagg cagcactttg cttaacagct tgcgcatatt
    13141 ttgcggctca cgcagctgtt cttcggtttc tcccggcatc accataaatt caaagcgacg
    13201 taccgcatga ggcagcgcgg cagaaacata cgggcgcacc ggatcacaac acaaatagat
    13261 atgcggcgta cttaacggat cgttggcgat atctaccaca atccactgat ttggcgcagt
    13321 tttaccttca aacggcacat tcagggtgcg acggacaaaa cttgccccac catcacaggc
    13381 caccagccat tgggctttga ccgtttcccg ctgcccttct gccgttttca ggtgcaaggt
    13441 cacttcgtca tcttgctggc tgaaggcctc cagctcgcgg gaaaacaggc agcgcacatt
    13501 cggaaaacgc gacaatcctt ccagcatcac cgcatcgacc tgaggctgaa taaaggcgtt
    13561 acggcgcggc cagccaaatt catcggtcat tggctgaata tcagcaaaac agcgaccttt
    13621 tggggtgaga aaacgcatcg cgtgccacgg cgtagtgtgc ggcagaacat tatcgaccag
    13681 gccgaccgac tgcatggtgc gcagcgcctc gtcatcaata ccaatcgcac gcgggtagtc
    13741 gatcaactta tcgagtttct ccaccaccag cacgtcaatg cccatctggc cgagatagtt
    13801 cgccatcatc agcccgaccg gaccggcacc agcgatcgcc acctgaacgc tatggttaac
    13861 agcaggctgg atgtcaggat gttgtattgc catttcagta cctcacgact cggacaaaat
    13921 gccgttgcgc gcacagtacg gcgcaactta ttttgttaaa aacatgtaaa tgatttttta
    13981 ttgtgcgctc agtataggaa gggtgttttc ggctacaatc aaaacatgcc cgaatgtgca
    14041 ccaagtgcac aacgttgttt taactataga aatgtcaatt aatatgcaga acaatgagca
    14101 gacggcatac aaaaccgtgc gcggcttaac ccgcggtcta atgttattaa atatgttaaa
    14161 taaacttgat ggcggtgcca gcgtcgggct gctggcggaa ctcagcggcc tgcatcgcac
    14221 cactgtgcgg cgactgctgg agacgctgca ggaagaggga tatgtccggc gtagtccctc
    14281 cgatgatagt ttccgtttga ccatcaaagt gcggcaatta agcgaaggat ttcgtgacga
    14341 acagtggatt tctgcactgg cggccccact gctgggcgat ctgttgcgcg aagtggtatg
    14401 gccgacagat gtgtccaccc tggatgttga tgcaatggtg gtacgcgaaa ctacccatcg
    14461 tttcagccgc ttatcttttc accgggcaat ggtcgggcga cggttaccgc ttctgaaaac
    14521 cgcctcgggc ctgacctggc tggccttttg cccggaacaa gaacgcaagg aattaatcga
    14581 gatgttagcc tcccgccccg gtgatgacta tcaactggca cgggaaccgt taaagctgga
    14641 agccattctg gcgcgcgcgc gcaaagaggg gtacggacag aactaccgcg gctgggatca
    14701 agaagaaaag atcgcctcta ttgccgtacc gctgcgcagt gaacaacggg tgattggctg
    14761 tctgaatctg gtgtatatgg cgagcgcaat gaccattgaa caggcagcgg aaaagcatct
    14821 tccggcgcta caacgggtgg caaaacagat cgaagaaggg gttgaatcgc aggctattct
    14881 ggtggccgga cggcgaagcg gcgtgcattt acgttgacac catcgaatgg cgcaaaacct
    14941 ttcgtggtat ggcatgatag cgcccggaag agagtcaatt cagggtggtg aatgtgaaac
    15001 cagtaacgct atacgatgtc gcagagtatg ccggtgtctc ttatcagacc gtttcccgcg
    15061 tggtgaacca ggcctgccac gtttctgcga aaacgcggga aaaagtggaa gcggcgatgg
    15121 cggagctgaa ttacattccc aaccgcgtgg cacaacaact ggcgggcaag cagtcgttgc
    15181 tgattggcgt tgccacctcc agtctggccc tgcacgcgcc gtcgcaaatt gtcgcggcga
    15241 ttaaatctcg cgccgatcaa ctgggtgcca gcgtggtggt gtcgatggta gaacgaagcg
    15301 gcgtcgaagc ctgtaaagcg gcggtgcaca atcttctcgc gcaacgcgtc agtgggctga
    15361 tcattaacta tccgctggat gaccaggatg ccattgctgt ggaagctgcc tgcgctaatg
    15421 ttccggcttt atttcttgat gtctctgacc agacacccat caacagtatt attttctccc
    15481 atgaagacgg tacgcgactg ggcgtggagc atctggtcgc attgggtcac cagcaaatcg
    15541 cgctgttagc aggtccatta agttctgtct cggcgcgtct gcgtctggcg ggctggcata
    15601 aatatctcac acgcaatcaa attcagccga tagcggaacg ggaaggcgac tggagtgcca
    15661 tgtccggttt tcaacaaacc atgcaaatgc tgaatgaggg catcgttccc actgcgatgc
    15721 tggttgccaa cgatcagatg gcgctgggcg caatgcgcgc cattaccgag tccgggctgc
    15781 gcgttggtgc ggatatctcg gtagtgggat acgacgatac cgaagacagc tcgtgttata
    15841 tcccgccgtt aaccaccatc aaacaggatt ttcgcctgct ggggcaaacc agcgtggacc
    15901 gcttgctgca actctctcag ggccaggcgg tgaagggcaa tcagctgttg cccgtctcac
    15961 tggtgaaaag aaaaaccacc cttccgccca atacgcaaac cgcttctccc caggcgttgg
    16021 cagattcttt aatgcagctg gcgcgacaaa tttcccgact cgaaagcggg cagtgagcgc
    16081 aacgcaatta atgtgagtca gctcactcat taggcacccc aggctttaca ctttatgcgt
    16141 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg atacagctat
    16201 gactatgatt acggattcac tggccgtcgt attacaacgt cgtgactggg aaaaccctgg
    16261 cgttacccaa cttaatcgcc ttgcggcaca tccccctttc gccagctggc gtaatagcaa
    16321 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgctt
    16381 tgcctggttt ccggcaccag aagcggtgcc ggaaagctgg ctggagtgcg atcttcctga
    16441 cgccgatact gtcgtcgtcc cctcaaactg gcagatgcac ggttacgacg cgcccatcta
    16501 caccaacgtg acctatccca ttacggtcaa tccgccgttt gttcccgcgg agaatccgac
    16561 aggttgttac tcgctcacat ttaatgttga tgaaagctgg ctacaggaag gccagacgcg
    16621 aattattttt gatggcgtta attcggcgtt tcatctgtgg tgcaacgggc gctgggtcgg
    16681 ttacggccag gacagccgtt tgccgtctga atttgacctg agcgcatttt tacgcgccgg
    16741 agaaaaccgc ctcgcggtga tggtactgcg ctggagtgac ggcagttatc tggaagatca
    16801 ggatatgtgg cgaatgagcg gcattttccg tgacatctcg ttgctgcaca aaccgaccac
    16861 acaaatcagc gatttccatg ttaccactcg ctttaatgat gatttcagcc gcgcggtact
    16921 ggaggcagaa gttcaggtgt acggcgagct gcgcgatgag ctgcgggtga cggtttcttt
    16981 gtggcagggt gaaacgcagg tcgccagcgg caccacgcct ttcggcggtg aaattatcga
    17041 tgagcgtggt ggttatgccg atcgcgtcac gctacgtctg aacgtcgaaa acccggcgct
    17101 gtggagcgcc gaaatcccga atatctatcg tgcggtggtt gaactgcaca ctgccgacgg
    17161 cacgctgatt gaagcagaag cctgcgatgt cggtttccgc gaggtgcgga ttgaaaatgg
    17221 tctgctgctg ctgaacggca agccgttgct gattcgcggc gttaaccgtc acgagcatca
    17281 tcctctgcat ggtcaggtca tggatgagca gacgatggtg caggatatcc tgctgatgaa
    17341 gcagaacaac tttaacgccg tgcgctgttc gcattatccg aaccatccgc tgtggtacac
    17401 cctgtgcgac cgctacggcc tgtatgtggt ggatgaagcc aatattgaaa cccacggcat
    17461 ggtgccaatg aatcgtctga ccgatgatcc gcgctggtta ccggccatga gcgaacgcgt
    17521 aacgcgaatg gtgctgcgcg atcgtaatca cccgagtgtg atcatctggt cgctggggaa
    17581 tgaatcgggc cacggcgcta atcacgacgc gctgtatcgc tggatcaaat ctgtcgatcc
    17641 ttcccgcccg gtgcagtatg aaggcggcgg agccgacacc tccgcaaccg atattatttg
    17701 cccgatgtac gcgcgcgtgg atgaagacca gccctttccg gctgtgccga aatggtccat
    17761 caaaaaatgg ctttcgctgc ctggagaact gcgcccgctg atcctttgcg aatatgccca
    17821 cgcgatgggt aacagtcttg gcggcttcgc taaatactgg caggcatttc gtcagtaccc
    17881 ccgtttacag ggcggcttcg tctgggactg ggtggatcag tcgctgatta aatatgatga
    17941 aaacggcaac ccgtggtcag cttacggcgg tgattttggc gatacgccaa acgatcgcca
    18001 gttctgcatg aacggtctgg tctttgccga ccgcacgccg catccggcgc tgacggaagc
    18061 aaaacaccag cagcagtttt tccagttccg tttatccggg cgaaccatcg aagtgaccag
    18121 cgaatacctg ttccgtcata gcgataacga gctcctgcac tggtcggtgg cgctggatgg
    18181 caagccgctg gcaagcggtg aaatgcctct ggatgttgct ccacaagata aacagttgat
    18241 tgaactgcct gaactaccgc agccggagag cgccggacaa ctctggctta cggtacacgt
    18301 agtgcaaccg aacgcgaccg catggtcaga agccggacac atcagcgcct ggcagcaatg
    18361 gcgtctgacg gaaaacctca gcgtgacact cccctccgcg ccccacgcca tcccgcaact
    18421 gaccaccagc gaaacggatt tttgcatcga gctgggtaat aaacgttggc aatttaaccg
    18481 ccaatcaggc tttctttcac agatgtggat tggcgatgaa aaacaactgc tgacgccgct
    18541 gcgcgatcag ttcacccgcg caccgctgga taacgacatt ggcgtaagtg aagcgacccg
    18601 cattgaccct aacgcctggg tagaacgctg gaaggcggcg ggccattacc agaccgaagt
    18661 ggcgttgttg cagtgcacgg cagatacact tgccgacgcg gtgctgatta cgaccgctca
    18721 cgcgtggcag catcagggga aaaccttatt tatcagccgg aaaacctacc ggattgatgg
    18781 tagtggtcaa atggcgatta ccgttgatgt tgaagtagcg agcgatacac cgcatccggc
    18841 acggattggc ctgacctgcc agctggcgca ggtcgcagag cgggtaaact ggctcggatt
    18901 agggccgcaa gaaaactatc ccgaccgcct tactgccgcc tgttttgacc gctgggatct
    18961 gccattgtca gacatgtata ccccgtacgt cttcccgagc gaaaacggtc tgcgctgcgg
    19021 gacgcgcgaa ttgaattatg gcccacacca gtggcgcggc gacttccagt tcaatatcag
    19081 tcgctacagc caacaacaac tgatggaaac cagccatcgc catctgctgc acgcggaaga
    19141 aggcacatgg ctgaatatcg acggtttcca tatggggatt ggtggcgacg actcctggag
    19201 cccgtcagtg tcggcggaat tccagctgag cgccggtcgc taccattacc agttggtctg
    19261 gtgtcaaaaa taataataac cgggcaggcc atgtctgccc gtatttcgcg taaggaaatc
    19321 cattatgtac tatttaaaaa acacaaactt ttggatgttc ggtttattct ttttctttta
    19381 cttttttatc atgggggcct acttcccgtt tttcccgatt tggctacatg acatcaacca
    19441 tatcagcaaa agtgatacag gtattatttt tgccgctatt tctctgttct cgctattatt
    19501 ccaaccgctg tttggtctgc tttctgacaa actcgggctg cgcaaatacc tgctgtggat
    19561 tattaccggc atgttagtga tgtttgcgcc gttctttatt tttatcttcg ggccactatt
    19621 acaatacaac attttagtag gatcgattgt tggtggtatt tatcttggct tttgttttaa
    19681 cgccggtgcg ccagcagtag aggcatttat cgagaaagtc agccgtcgca gtaatttcga
    19741 atttggtcgc gcgcggatgt ttggctgtgt tggctgggcg ctgtgtgcct cgattgtcgg
    19801 catcatgttc accatcaata atcagtttgt tttctggctg ggttctggct gtgcactcat
    19861 cctcgccgtt ttgctctttt tcgccaaaac ggatgcgccc tcttccgcca cggttgccaa
    19921 tgcggtaggt gccaaccatt cggcatttag ccttaagctg gcgctggaac tgttcagaca
    19981 gccaaaactg tggtttttgt cactgtatgt tatcggcgtt tcctgcacct acgatgtttt
    20041 tgaccaacag tttgctaatt tctttacttc gttctttgcc acaggtgaac agggtacgcg
    20101 ggtatttggc tacgtaacga caatgggcga attacttaac gcctcgatta tgttcttcgc
    20161 gccgctgatc attaatcgca tcggtgggaa aaacgccctg ctgctggctg gcactattat
    20221 gtctgtacgt attattggct catcgttcgc cacctcagcg ctggaagtgg ttattctgaa
    20281 aacgctgcat atgtttgaag taccgttcct gctggtgggc tgctttaaat atattaccag
    20341 ccagtttgaa gtgcgttttt cagcgacgat ttatctggtc tgtttctgct tctttaagca
    20401 actggcgatg atttttatgt ctgtactggc gggcaatatg tatgaaagca tcggtttcca
    20461 gggcgcttat ctggtgctgg gtctggtggc gttgggcttc accttaattt ccgtgttcac
    20521 gcttagcggc cccggtccgc tttctctact gcgtcgtcag gtgaatgaag tcgcttaagc
    20581 aattaatgtc ggatgcggcg cgagcgcctt atccgaccaa catatcagga cggaatgatc
    20641 gcaatgaaca tgccaatgac cgaaagaata aaagcaggca agctatttac cgatatgtgc
    20701 gaaggcttac cggaaaaaag acttcgtggg aaaacgttaa tgtatgagtt taatcactcg
    20761 catccatcag aagttgaaaa aagagaaagc ctgattaaag aaatgtttgc cacggtaggg
    20821 gaaaacgcct gggtagaacc acccgtccat ttctcttacg gttccaacat ccatataggc
    20881 cgcaattttt atgcaaattt caatttaacc attgtagacg actacacggt aacgatcggt
    20941 gataacgtat tgattgcacc caatgttact ctttccgtta cgggacaccc tgtacaccat
    21001 gaattgagaa aaaacggtga gatgtactct tttccgataa cgattggcaa taacgtctgg
    21061 atcggaagtc atgtggtcat taatccaggc gtcaccatcg gggataattc tgttattggc
    21121 gcgggtagtg tcgtcataaa agacattcca ccaaacgtcg tggctgctgg cgttccttgc
    21181 cgggttat
//
LOCUS       DAZGQL010000087        21055 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.087, whole genome shotgun sequence.
ACCESSION   DAZGQL010000087 DAZGQL010000000
VERSION     DAZGQL010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21055
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.087"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            35..111
                     /locus_tag="ACHKIR_003763"
     tRNA            35..111
                     /locus_tag="ACHKIR_003763"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:69..71,aa:Arg,seq:acg)
     gene            392..958
                     /gene="yqaB"
                     /locus_tag="ACHKIR_003764"
     CDS             392..958
                     /gene="yqaB"
                     /locus_tag="ACHKIR_003764"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HFO9991442.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDVQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            955..1383
                     /locus_tag="ACHKIR_003765"
     CDS             955..1383
                     /locus_tag="ACHKIR_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HFO9991443.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            1456..3012
                     /gene="gshA"
                     /locus_tag="ACHKIR_003766"
     CDS             1456..3012
                     /gene="gshA"
                     /locus_tag="ACHKIR_003766"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HFO9991444.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLPQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFIAEREASERRQQEMEAADTE
                     PFAVWLEKHA"
     gene            3162..3677
                     /gene="luxS"
                     /locus_tag="ACHKIR_003767"
     CDS             3162..3677
                     /gene="luxS"
                     /locus_tag="ACHKIR_003767"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFO9991445.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(3728..4840)
                     /locus_tag="ACHKIR_003768"
     CDS             complement(3728..4840)
                     /locus_tag="ACHKIR_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO9991446.1"
                     /translation="MIWQLTDDKRWSALRQRFSWVEEMHHTPQDPEHHGEGDVGVHTE
                     MVLNALVTQPEFQQLPAQQQEVLWAAALLHDVEKRSTTVQENGRIQSPGHARRGELTA
                     RQILWRDIPTPFVLREQIVALVRLHGLPLWLLERPEPERLLLTAAMRIDTRLLALLAR
                     ADLLGRQSPDQQSMLERIDLFELFCHEQQCWGKMRPFVSDSARWHYLTHEQSSPDFVP
                     WEAEPFEVILLCGLPGMGKDRYISEQCQGMDVISLDDMRRRINASPDDKTATGRIVQQ
                     AKEEARVFLRQKKPFIWNATNITRQLRSQLISLFTAYGARVKIVYLEVPWAQWKQQNA
                     RREYAVPEAVVMRMASRLEVPQPDEAHSVEYRMTDR"
     gene            complement(4837..5544)
                     /locus_tag="ACHKIR_003769"
     CDS             complement(4837..5544)
                     /locus_tag="ACHKIR_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase family protein"
                     /protein_id="HFO9991447.1"
                     /translation="MNTQRKYGRTWHYPFSPGTTSDDRINADYWQDLQAITQLVHTEK
                     LDGENNCLNRYGVFARSHAAPTQSAWTYKIRQRWQLLKNDLGDLELFGENLYAVHSIE
                     YRALEQDFYLFAVRCQDMWLSWEEVQFYAALFDFPCVPEISGPQPGNDEKSWQRDFLA
                     LTNARGAFDPWDTQTGQPCTLEGIVSRNRDAFSVADFSHNVFKYVRKNHVKTTVHWKR
                     HWQRARMAHEFDYGEQS"
     gene            complement(5802..7340)
                     /gene="emrB"
                     /locus_tag="ACHKIR_003770"
     CDS             complement(5802..7340)
                     /gene="emrB"
                     /locus_tag="ACHKIR_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HFO9991448.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(7357..8529)
                     /gene="emrA"
                     /locus_tag="ACHKIR_003771"
     CDS             complement(7357..8529)
                     /gene="emrA"
                     /locus_tag="ACHKIR_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HFO9991449.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            complement(8656..9186)
                     /gene="emrR"
                     /locus_tag="ACHKIR_003772"
     CDS             complement(8656..9186)
                     /gene="emrR"
                     /locus_tag="ACHKIR_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HFO9991450.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            complement(9277..9612)
                     /gene="ygaH"
                     /locus_tag="ACHKIR_003773"
     CDS             complement(9277..9612)
                     /gene="ygaH"
                     /locus_tag="ACHKIR_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HFO9991451.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            complement(9602..10339)
                     /gene="ygaZ"
                     /locus_tag="ACHKIR_003774"
     CDS             complement(9602..10339)
                     /gene="ygaZ"
                     /locus_tag="ACHKIR_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HFO9991452.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            complement(10463..11647)
                     /locus_tag="ACHKIR_003775"
     CDS             complement(10463..11647)
                     /locus_tag="ACHKIR_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9991453.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAACGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISDKILRTRTL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLPWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASVWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AI"
     gene            complement(11839..12831)
                     /gene="proX"
                     /locus_tag="ACHKIR_003776"
     CDS             complement(11839..12831)
                     /gene="proX"
                     /locus_tag="ACHKIR_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HFO9991454.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYGLTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWAEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            complement(12889..13953)
                     /gene="proW"
                     /locus_tag="ACHKIR_003777"
     CDS             complement(12889..13953)
                     /gene="proW"
                     /locus_tag="ACHKIR_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HFO9991455.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPAAAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            complement(13946..15148)
                     /gene="proV"
                     /locus_tag="ACHKIR_003778"
     CDS             complement(13946..15148)
                     /gene="proV"
                     /locus_tag="ACHKIR_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0031460 - glycine betaine transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HFO9991456.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKAALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            complement(15504..16463)
                     /gene="nrdF"
                     /locus_tag="ACHKIR_003779"
     CDS             complement(15504..16463)
                     /gene="nrdF"
                     /locus_tag="ACHKIR_003779"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFO9991457.1"
                     /translation="MKLSRISAINWNKITDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLSVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNLEKISQARREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            complement(16473..18617)
                     /gene="nrdE"
                     /locus_tag="ACHKIR_003780"
     CDS             complement(16473..18617)
                     /gene="nrdE"
                     /locus_tag="ACHKIR_003780"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFO9991458.1"
                     /translation="MATTTAERLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     MTTHVRPNSVAFSSQQQRLNWLVNEGYYDESVLNRYSSDFVITLFAHAHASGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLATQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDIERVYGKPFADVAISEHYAEL
                     VANERIRKKYINARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQSNWQPKTAKVGELFARSSITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            complement(18590..19000)
                     /gene="nrdI"
                     /locus_tag="ACHKIR_003781"
     CDS             complement(18590..19000)
                     /gene="nrdI"
                     /locus_tag="ACHKIR_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFO9991459.1"
                     /translation="MSQIVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            complement(18997..19242)
                     /gene="nrdH"
                     /locus_tag="ACHKIR_003782"
     CDS             complement(18997..19242)
                     /gene="nrdH"
                     /locus_tag="ACHKIR_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFO9991460.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAANA"
     gene            complement(19489..19818)
                     /locus_tag="ACHKIR_003783"
     CDS             complement(19489..19818)
                     /locus_tag="ACHKIR_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HFO9991461.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            19970..20314
                     /locus_tag="ACHKIR_003784"
     CDS             19970..20314
                     /locus_tag="ACHKIR_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2002 family protein"
                     /protein_id="HFO9991462.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            complement(20351..20800)
                     /gene="alaE"
                     /locus_tag="ACHKIR_003785"
     CDS             complement(20351..20800)
                     /gene="alaE"
                     /locus_tag="ACHKIR_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HFO9991463.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADIMAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
BASE COUNT         5220 a         5345 c         5482 g         5008 t
ORIGIN      
        1 tatcaatatc agcagtaaaa taaattttcc caatgcatcc gtagctcagc tggatagagt
       61 actcggctac gaaccgagcg gtcggaggtt cgaatcctcc cggatgcacc atattctccg
      121 taaccttcag caatgaaggt atcgaagagg tagcggtatt aaccgcaggc accagggagg
      181 ataacgttgc gccagcagcg gcccgaaggg cgaacgccag tgagtcatcc tcccggatgc
      241 accatcttct ccaaaaaatt tccttcaaaa atatctcatt tctaaataac tactttatcc
      301 atttcctgaa tattcttctc cttctccatc agcgacaaaa tcaagcaatt acgctaaagt
      361 agcgtcatat ttttcgactt gcgagaagac catgtacgag cgttatgcag gtttaatttt
      421 tgatatggat ggcacaatcc tggatacgga gcctacgcac cgtaaagcgt ggcgcgaagt
      481 attagggcac tacggtcttc agtacgatgt tcaggcgatg attgcgctta atggctcgcc
      541 cacctggcgt attgctcagg cgattattga gctgaatcag gccgatctcg acccgcatgc
      601 gttagcgcgt gaaaaaacag aagcagtaag aagtatgctg ctggatagtg tcgagccgct
      661 tcctcttgtt gaagtggtga aaagctggca tggtcgtcgc ccaatggctg taggaacggg
      721 gagtgaaagc gccatcgctg aggcattgct ggcacacctg ggattacgcc gttattttga
      781 cgccgtcgtc gctgccgatc acgtcaaaca ccataaaccc gcgccagaca catttttgtt
      841 gtgcgcgcag cgtatgggcg tgcaaccgac gcagtgtgtg gtcttcgaag atgccgattt
      901 cggtattcag gcggcccgtg cagcaggcat ggacgccgtg gatgttcgct tgctgtgagt
      961 gaagcgttat cgcttttctc gttgtttgcc agtagttttc tcagcgctac actattaccc
     1021 ggcaactccg aagtcgtgct ggtggcaatg ttgctttccg ggatcagtca tccctgggtt
     1081 ttagttttaa cagcaacaat gggtaatagc cttggagggt taactaacgt tatccttggg
     1141 cgtttctttc cattgcgtaa aacatcgcgc tggcaagaga aagctaccgg ctggctgaaa
     1201 cgctatggtg cagtcacgct attattaagc tggatgcccg tggttggcga tttactgtgt
     1261 ctgttagcgg gatggatgcg catctcgtgg ggaccggtaa tctttttttt gtgccttggt
     1321 aaagcgttac gctatgttgc ggttgcagca gctaccgttc agggcatgat gtggtggcac
     1381 taattgtagg cctgcacata tggtcaccat tacagttatg ctaattaaaa cgattttgac
     1441 aggcgggagg tcaatttgat cccggacgta tcacaggcgc tggcctggct ggaaaaacat
     1501 cctcaggcgt taaaggggat acagcgtggg ctggagcgcg aaactttgcg tgttaatgct
     1561 gatggcacac tggcaacaac aggtcatcct gaagcattag gttctgcact gacccataaa
     1621 tggattacta ccgattttgc ggaagcattg ctggaattta ttacaccagt ggatggtgat
     1681 attgaacata tgctgacctt tatgcgcgat ctgcatcgct atacggcgcg caatatgggc
     1741 gatgagcgga tgtggccgtt aagtatgcca tgctacatcg cagaaggtca ggacatcgaa
     1801 ctggcacagt acggcacttc taacaccggt cgctttaaaa cgctgtaccg tgaagggctg
     1861 aaaaatcgct acggcgcgct gatgcaaacc atttccggcg tgcactataa tttctctttg
     1921 ccaatggcat tctggcaagc gaagtgcggt gatatctcgg gcgctgatgc caaagagaaa
     1981 atttctgcgg gctatttccg cgttatccgc aattactatc gtttcggttg ggtcattcct
     2041 tatctgtttg gtgcgtctcc agcgatttgt tcttctttct tgcaaggaaa accaacgtcg
     2101 ctgccgtttg agaaaaccga gtgcggtatg tattacctgc cgtatgcgac ctctcttcgt
     2161 ttgagcgatc tcggctatac caataaatcg caaagcaatc ttggtatcac cttcaatgat
     2221 ctctacgagt acgtggcggg ccttaaacag gcaatcaaaa cgccatcgga agagtacgcg
     2281 aagattggta ttgagaaaga cggtaagcgg ctgcaaatca acagcaatgt gctgcagatt
     2341 gaaaacgaac tgtacgcgcc gattcgtcca aaacgcgtta cccgcagtgg tgagtcgcct
     2401 tctgatgcgc tgttacgtgg cggcattgaa tatattgaag tccgctcgct ggatatcaac
     2461 ccgttctcgc cgattggtgt agatgaacaa caggtgcgat tcctcgacct gtttatggtc
     2521 tggtgtgcgc tggctgacgc accggaaatg agcagtagcg aacttgcctg tacacgcgtt
     2581 aactggaacc gggtgatcct cgaaggtcgc aaaccgggcc tgacgctggg tatcggctgc
     2641 gaaactgcgc agttcccgtt accgcaggtg ggtaaagatc tgttccgcga tctgaaacgc
     2701 gtcgcgcaaa cgctggatag cattaacggc ggcgaagcgt atcagaaagt gtgtgatgaa
     2761 ctggtcgcct gcttcgataa tcccgatctg actttctctg cccgtatctt aaggtctatg
     2821 attgatactg gtattggcgg aacaggcaaa gcgtttgcag aagcctaccg taatctgctg
     2881 cgtgaagagc cgttggaaat tctgcgcgaa gaggatttta tagccgagcg cgaggcgtct
     2941 gaacgccgtc agcaggaaat ggaagccgct gataccgaac cgtttgcggt gtggctggaa
     3001 aagcacgcct gacagaaaag aaaaaggcca ctcgtgagtg gccaaaattt catctctgaa
     3061 ttcagggatg atgataacaa atgcgcgtct ttcatatact cagactcgcc tgggaagaaa
     3121 gagttcagaa aattttaaaa aaaattaccg gaggtggcta aatgccgttg ttagatagct
     3181 tcacagtcga tcatacccgg atggaagcgc ctgcagttcg ggtggcgaaa acaatgaaca
     3241 ccccgcatgg cgacgcaatc accgtgttcg atctgcgctt ctgcgtgccg aacaaagaag
     3301 tgatgccaga aagagggatc cataccctgg agcacctgtt tgctggtttt atgcgtaacc
     3361 atcttaacgg taatggtgta gagattatcg atatctcgcc aatgggctgc cgcaccggtt
     3421 tttatatgag tctgattggt acgccagatg agcagcgtgt tgctgatgcc tggaaagcgg
     3481 caatggaaga cgtgctgaaa gtgcaggatc agaatcagat cccggaactg aacgtctacc
     3541 agtgtggcac ttaccagatg cactcgttgc aggaagcgca ggatattgcg cgtagcattc
     3601 tggaacgtga cgtgcgcatc aacagcaacg aagaactggc gctgccgaaa gagaagttgc
     3661 aggaactgca catttagtca gtttaagcag aagaggccgg gctggtgaaa acaccgcccg
     3721 gcttttgtta cctgtcagtc atccgatatt ccacgctatg tgcttcatca ggttgaggaa
     3781 cttccagcct tgaggccatc cgcatcacga ccgcttcagg aactgcatac tcgcgtctgg
     3841 cattttgctg cttccattgc gcccatggca cttccagata aacgattttt actcgtgcgc
     3901 cgtaagccgt aaacagactg atgagctggc tgcgcagctg gcgggtaatg ttcgttgcat
     3961 tccagatgaa cggctttttc tggcgtaaaa aaacgcgcgc ctcttctttt gcctgttgca
     4021 caatacgccc ggtggcggtt ttgtcgtccg gactggcatt gattcgccgc cgcatatcat
     4081 caaggctgat aacgtccatt ccctggcact gttcactgat atagcgatct ttgcccatac
     4141 ccggtaagcc acacagcaaa ataacctcaa aaggttcagc ctcccaggga acaaaatcag
     4201 gagaactttg ctcatgagtc aggtagtgcc agcgtgcgga atcagaaacg aacgggcgca
     4261 tttttcccca gcattgttgc tcatggcaaa acagctcgaa cagatcgatg cgttccagca
     4321 ttgattgctg atccgggctt tggcgaccaa gcagatcggc acgagcaagc aaagcgagca
     4381 gacgcgtgtc gatgcgcatt gcagccgtga gcagcaaacg ctccggttcc ggacgttcca
     4441 gtaaccatag cggtaaccca tgcagacgca ccagcgcaac aatttgctcg cgtagcacga
     4501 acggcgttgg gatatcgcgc cataaaattt gtcgggccgt cagttcgccc cgacgagcat
     4561 ggccaggcga ctgaatacgt ccgttttcct gcacggtagt gctgcgcttt tcaacatcat
     4621 gcaataacgc cgccgcccat aaaacttcct gctgttgagc ggggagttgc tggaattctg
     4681 gctgagtaac gagggcgttc agcaccattt cggtatgcac gccgacgtca ccttcaccat
     4741 gatgctccgg gtcttgtggc gtgtgatgca tctcctcaac ccagctaaaa cgctgacgta
     4801 gcgccgacca gcgtttgtcg tcggtaagct gccaaatcat gactgttctc cataatcgaa
     4861 ctcatgggcc atccgcgccc gttgccagtg tcgtttccag tggacggtgg ttttgacatg
     4921 atttttgcgc acatacttga agacgttatg cgaaaagtca gcgacggaaa acgcatcacg
     4981 attcctgctg acaatgcctt ccagcgtaca gggttgcccg gtttgcgtat cccagggatc
     5041 gaacgcgccg cgcgcattgg tcagcgccag aaaatcacgt tgccagcttt tttcatcatt
     5101 ccccggttgc gggccggaga tttcgggaac gcaggggaaa tcaaacagag cggcgtaaaa
     5161 ctgcacctct tcccagctca gccacatatc ctggcagcga acggcaaaca gataaaaatc
     5221 ctgctccagg gcgcgatatt caatcgaatg cacggcataa agattttcac caaacaactc
     5281 cagatcgccg agatcgtttt tcagcagttg ccagcgttgg cgaattttat aggtccatgc
     5341 cgactgcgta ggcgcggcat gggaacgggc aaacacgccg tagcggttaa ggcagttatt
     5401 ttcaccgtcg agtttttctg tgtgcaccaa ttgcgtgatg gcctgtaaat cctgccagta
     5461 atcggcatta atgcggtcat cgctggtggt gccgggggag aaaggataat gccaggttct
     5521 cccgtatttt cgttgggtat tcataataat ccattatttt gtgtgtaata aatgtacgcc
     5581 cgcgagggtg cgagttatgg ggttaaaaga aaaatgtgag tatcacccgc gaaagcgagg
     5641 gcgaaagaaa aagagaatta acaacctgag cgaatgctca gaaccggtta atggtgaatg
     5701 gaagagtttg gggcggcgaa aagatacacc gaatgagtgg ataaaacaac cgtttgcaaa
     5761 atgagcaaat aaaaagggag ccgcagctcc ctttgacaac cttagtgcgc accgcctccg
     5821 ccgccacctg caccaaatgg cggtttagca aaccacacca gccccagcag gacgaggaat
     5881 attccggctg acatccagaa gatctcattg gcggaaataa tcagcccctg attagtgatc
     5941 tgctgggcaa tccagcctga tgcctgttgt tgcgtcatcc ccagcccttc cagttggctg
     6001 tacatcgcct gagcgttcgg gttaaacggg tttaccgact cggttaactg tgcgtggtgc
     6061 atcgactcgc ggttagtcca catggtggtg gtgatggatg tgccgataga ccccgccagc
     6121 gttcgcgtaa agttggagag gctcgatgcc gccgccagtc gttccggtgg caaaccggac
     6181 agcgtaatgg tggtcagcgg cataaagaag caggccaccg caaacccctg gataaactgc
     6241 ggccaggccg acgcgccaaa atccatacct ggttcaaagg tataggcgcg ccagtagaag
     6301 cagacggcat acataataaa gctgaaggtt accagccgcc gcatatccag tttatgcgcg
     6361 aagcggccga taatcggcga caggatcacc ggaataatcc ctaccggcgc agaggccaga
     6421 cctgcccacg ttgccgtgta accgtagacc tcctgcaaca actgcggcag cagaacaatc
     6481 gcgccgaagt agagcatata cgcgaggctg atacacaagc agccgatggt gaagttgcgc
     6541 gacttaaaca acgacagatc gactatcggg ttatcgtcgg tcagctccca gacaatcagg
     6601 aagcagatag ccaccactgc caccacggta aggatgataa tttcctgtga tgagaaccag
     6661 tccagctctt taccgcggtc gagcataatc tgcaggctac cgataccaat aaccagcagt
     6721 gccagcccca cggcatcaat ccgccgccgt tcggtgcggg tttcacgtcc gcgcagggtt
     6781 tgcagtgtca tcaacaccac cgccacgcca atcggtacgt tgatgaagaa tatccagccc
     6841 cagtggtaat tgtcactgat atacccgccg aggatcgggc cgcaaattgg cgcgacaatc
     6901 accgtcatcg accacaacgc cagcgcgatc gagcgtttgg ctggcgggta gttattaagc
     6961 aatagacttt gcgaaagcgg gatcagcggc ccggcgacaa tcccctgaat cacgcggaag
     7021 aagatcagca tattcaggct gctggagaca ccacacgccc acgacgcaat agcaaaggcg
     7081 atggtggacc agaggaacag tttcacttcc ccgacgcgct ttgccagcca gccggtaagc
     7141 gggatcgaga tggcattcgc caccccgaaa gaagtgatta cccacgttcc ctggctgagc
     7201 gatgagccca gattcccggc gatagtgggg atcgccacgt tagcaatggt ggagtccagc
     7261 acctgcatga atgtcgccag tgacagcgca atcgtcataa tgaccagttg cgcgccttcc
     7321 agcggttttt gctgttgcat cacacgcacc tctggattag ccagcgttag cttttacgat
     7381 atcgtcgatc agtttattga caggtgccag gctgatttca cgcgcggtgc ttaccgctac
     7441 cggtgtggaa cgtactttat ttgccagtac ctgaccgtca cggttagtgg tattgacgct
     7501 caccagcgtg gacaaaccga tacgcagcgg gtattgctcg agctgtttct ggtccagttc
     7561 aatacgaaca ggcagacgct gaacgacttt gatccagtta ccggttgcat tttgcgccgg
     7621 aagcagtgag aacgcgctac ctgtgcccat atccagacca accacttttc cggtgtattt
     7681 cacatcatcg ccgtaaatat cggtggtgat ggtgaccggc tgaccgatac gcatattggc
     7741 aatctgcgtc tctttaaagt tggcatccac ccacatattg gtggctggaa cgaccgccat
     7801 cagcggcgtc gttgggctaa tttgcgcccc aggctgtacc gcgcggcggg agacataacc
     7861 ggtcatcgga ctgacaatac gggtacgctc cagcgccagc caggcgttac gtacttcggt
     7921 ggcagcctgt tgcacggcag gctgatcttc cagtttagtc cccagaatca tcgcctgatt
     7981 ggcattgtat tgttgaatag cgacgtccag ttgtgcctgg gcactggtaa cggcgtcgcg
     8041 ggcgtgttgc agctcttcgc gaccaatcag gttggcattg cccagcggca cacggcggtt
     8101 gtagtcgctt tgtgctttcg cgagggcgat tttttgcacc tcaatattcg cctgcaactg
     8161 tttgctgtta atcatcagct ggtgggtttg gcgaacgctg gaagccagtg cagttttggc
     8221 tttttcaaac gcctggcgag catctgtcgg gtcgagagtg accagcacat cgccttcttt
     8281 tacaaaatcg gtgttatcgg cccagacttt cgtcacgctg ccagacacct gagacataat
     8341 ttgaatctga ttccctgcca cgtatgcgtc atcagtttct tcgaaatgac gcagtaccaa
     8401 aaaccaataa atccctatcg ctacggcaat aattataaag agcaaggtga gaaggaggag
     8461 cagacgctta cgtttgccgc tcttctttac cggttgctgc ggggtttgag tctccgcatt
     8521 tgcgctcata ttgttctcca cgatcttctt aactcatcgg ctgagccgac ctgcttgcca
     8581 aaaaggccag catgttgcga tgctggccag tcattttttc ttttataaat ctggattttt
     8641 gagcgagatg acgcgttagc tcatcgcttc gagaaccaca ccgtcttgtt ccatctggtc
     8701 gagacgggag agcaatttgc gggtgatttg ctcgagctga tctttttctg ttgtgctgag
     8761 cgcggaccag agttgatgca ggcagttatg ctgcggtggt aaaacctcgc gcaaaaactc
     8821 gtgacctttt tccgttaatt gcagatgcag gcagcggcga tcgttatcgc tttcacgacg
     8881 ttcgatccaa ccgcgttttt ccagttcatc ggcaatacgc gtcgcgttgg tacgggatga
     8941 tccaagagca caactcaatt cagaaggctg aatactgtgg ttttcctgag actccagcgt
     9001 aatcaacgcc ataaacaacg tctcgttaat cccctgagcc ttcagcattt tattgcggtt
     9061 ctccagcagc ttgctttgca tgtgcatgca aagacgggtc agaaggatct cctgataagg
     9121 aaaatcttcg tggcggctgg cgcgaaattt tagcatttgt tcaatgggcg taaacgaact
     9181 atccatttgg gtatgacctc attaatttca gccgatatag taacgacagt gacaaataaa
     9241 gtaaatgtat tgttttagaa aaatgattct tgtgggttat ataatcgcca tcactttcca
     9301 ggcgagccca taggccagcg cactaagcag tgttgggatg ataatgctgc gtgttttata
     9361 gaaactggca cccagtaccg cgaagccgac cagagtgggc acgaaacggc gtgtatcgtg
     9421 catcacttct ggtgcggtag agacaaccag cagagcgcat atcgaggcga tgccaatggt
     9481 gtcgagcaaa atacctaccg cgccacgttt ggttgggcgg gcattaccca cacgcaggcg
     9541 cagcggcaaa tagcggaagc aataattcgc cgcgccaacc agtaacccaa gcagcagaac
     9601 ctcatagctc atcgggcgct ccttgccaga atgcctggat taacgcagtg aggcagccac
     9661 agacaatgcc tgccagaatg gcgacgggaa tagaaaatag cgttacgcct gcaagggcac
     9721 caactaacgc tgcggtaacg caaagagatt gtttgcgctg gaaagaggcg agcaggaaac
     9781 tcataaagag tgccggaagc ataaaaccta atgctgcttc aacggcggga tatccttgca
     9841 gcaagccgct gccggagaat gcccctatta ccgtaccaaa tacccacgat gaccatgaac
     9901 tgaaggcaat gccgatcatc cagttctcgc tccagcggcg attattgcgt accagttttg
     9961 cggttgcggc ggcaaaaacc tcatccgtca ggccaaacgc ccacagggca gttttcgatt
    10021 tttgcagacg ctgaataata cggctacgca gtgacgggcc atacaaaaca tggcgaacat
    10081 ccattgccat gacggtcagt gcagcaaccc acaaactact cccggctgcc agcatcgcgg
    10141 taatgacgaa ctggctcgcg cctgcataaa tgatgcagga gaaaaaaacg ctttcgagag
    10201 gagagaatcc cagacgggtc gcattcagac cgaacgcaaa ggccaccgga atataactaa
    10261 taacaatcgg taaactgtct ttgcatcctt ccatgaaggt cgccgaacca ggagcaggct
    10321 gtggagtagg gctttccata gagtgtacgc ttaacgattg ttaatattat aaatagactg
    10381 aatgaatatc ttaaccttat cagactgatg ggcttcttaa cacccttata agtgtaaagc
    10441 cacgaaaacg gttgctgatt gcttaaattg cggcttcctg acgatgaaaa cctcgccacc
    10501 agaccagtaa gttaaccagg gcaatcgtcg cgccagccag acaaacgcca gcccaaccgc
    10561 catgttgcca gactgaggct gaaattagcg aaccggcggc accgccaatg aagtagctgg
    10621 tcatgtaacc tgcggtcagg cgattacgcg catcagggtg tattcgataa attaccgtct
    10681 ggttagtgat atgcacgccc tgcacggtga gatccagcac caggatgccg ataatcagcg
    10741 ccagtacgga agtgtgacca aaccagatcg ccagccatgg aagtaacagc agcagcagac
    10801 cgaaagttgt ggtgtggtgc gatttgccct tatcggcaaa tccgcccgcc ggacgagcgc
    10861 ccaacgctcc ggctgctccc gcaagtccaa acagaccaat gacgccatcg ctgtaattaa
    10921 aaggcggagc ggcgagcaaa aaggccatcg aggtccagag aatgctgaag ttggcaaagg
    10981 tcaggcagcc cagcaacgtg cgggtgcgca gaattttatc gctgataaac atgctgaaaa
    11041 cggaaccaag caactgtggg tagttgaggt gggtttctga tttcatttgt ggcagaccac
    11101 gccataatgc cagcgccatc agtgccatta acaccgaagc aacccaaaag acggtgcgcc
    11161 agccgccgag gtttgccagc aacccggcaa cggttcgtgc cagcaagatc cccaacagca
    11221 gcccgctcat aatggtgccg accactttgc cgcgtttgtc cggtgaagcc agcgtcgctg
    11281 ccagcggaac cagaatttgt gccacgaccg agaataaacc ggttaatgcc gtaccgagga
    11341 tcatcatcgc cagcgactga ctgctggcag tgattaacat gccgcatgcc gccagtaagg
    11401 tcatcgagac aatcaggcgg cggcgttcaa acatatcacc gaggggaaca agaaacagca
    11461 gacctgcggc atagcccaac tgcgcggcgg taacaataaa gcctgccgaa ctggcggaaa
    11521 gggaaaagtt acgcgcgatg gtgtcgagca atggctgggc gtaatagtta ctggctaccg
    11581 ccaggccggt ggcgatagac atcagcacga tcagcgccgg gctaagctca tgattaggtt
    11641 tagtcattat ttttgttcat caggtgatga ttaagctgga aatcaataat agcggatgtt
    11701 gatgaatatg ggatgaaaga tagattgttg aaatgtgcgg tgtggtttgt aggtatgata
    11761 agacgcacca gcgtcgcatc aggcattgtg tacagagcgg cggatgaggc aaaaacgcct
    11821 tatccgcccg aataaaaatt acttctgcgc tgccagcgcc tcattcaccc agccatcgaa
    11881 ctgctgctgg tgggctttga tccagccatc aacgtggccc tgaatatcgc cttctgaggc
    11941 tttgccgtca tgcataatgg cgttctgggc gttaatatca gccactggta actgcataat
    12001 ggcaaacagt ttcgctgctg ccgggttttt ctcggcccag gctttgttgg caacgatatg
    12061 catggtactg accgggaagc cgtaattcgc gccattcggc agtttggtat cggcattttt
    12121 atcgcccggc agtgcggaga acggcacctg caaccagacc acatctttcc ccggtttcag
    12181 ttcgttactc acccagtacg gcgtccaggt gtagtaaaac accggcttac cctctttgta
    12241 acgactgatg gtgtcagcca tcatcgctgc gtagttcccc tgattatgcg tcacggtatt
    12301 ggtcagtcca tacgcggcaa gctggtggtt gatcgcacct tcgcagcccc agcctgggtt
    12361 acagccggtt aaatccgctt taccgtcgcc gttggtatcg aacagtttgg cgatcttcgg
    12421 atctttaagt tgcgcgatgt tggtaatttt gtactggtcg gcagttttct tatcgatcag
    12481 gtaaccctgt gccgcgccgt taacaaatac tccttcacga taaaatttct tatcgccacc
    12541 ggcagcttcg tacatgttgt catgcagtgg cgtccagttc actgcggtga aggttgcatc
    12601 gccggaagca agcgaggtgt agccaacgtt gtaatccact tcgctgggtt tattgacggt
    12661 ataacctaat ttctccagcg cgcggctgac cagcagcgtc tggaaagttt cttcggtgat
    12721 ggtgctctga accggattaa cagtaatgcc tttgcccggc aggtcggcag caaaagtttg
    12781 tgtagagata agcgtggcaa acgctgtcgc aaaaagtacg ctatgtcgca ttgttattcc
    12841 tttttttggc agggtgataa tgcccggcgt caccgggcaa gtgcagagtt acttaatgaa
    12901 tgggcgggtc agcagaccga cagggccagt ggtgtaccag cgacggttgc cgcgactgcg
    12961 tgagtcgcgc ccaacggcct gcgtcaggcg gtcgaggata atggcgagga tcacaatccc
    13021 gacgccgcca acggtggcaa gccccatatc cagacgaccg ataccgcgaa gcaccatctg
    13081 acccagcccg ccgacggcaa tcatcgaggc gatgaccacc atagaaaggg ccagcatcag
    13141 cgtctggtta acgcccgcca taatggtcgg catcgccagc ggtaactgaa ctttgaacag
    13201 catctggcgc gggctggcac cgaatgagcg cgaggcttca atcagatccg ccggaacctg
    13261 gttaattccc agaatcgtca gacggataat cggcggcagc gcaaagatga tcgtcaccac
    13321 cacgcccggc acgttaccga taccaaacag catgacgatt ggtaccagat aaacaaacgc
    13381 gggcgtggtc tgcatggcat caagcagtgg acgaataatt ttcgccgctc gcggacttct
    13441 cgccagccag atccccaacg gcaaaccgat gacgatacag aacagcaggg cggttagcac
    13501 cagcgccaga gtaaccattg cctgtgacca ggcaccgatt gcgccgatgg caatcagcga
    13561 aaccagtgtc gccacgccca ttccgacacc ggaaatttgc caggcgatga gagcgaaaac
    13621 gataatcgcc accggagcgg gcatacccag cagcaattgc tggaaaccgt tgaggatgta
    13681 atcaaccgga acgcgcacgc cctgaaagac gggacgaaaa tgggtaacga cccagtcgat
    13741 cccttcagtg acccaactgt cgagcgggat cagcgtttta tggaacggat cgagaatatt
    13801 aaaatgctcg acgtttggcg caggcgtact ggtcagccag tcagcaccac cgccgtcagt
    13861 cggtgcagca gccggtgtac cccaggcgtc tgcggactgt gcggcactgt ccgccgctgg
    13921 cgtggtatcc cacggattat tttgatcagc cattatttac cccctcacga tctaaagcgc
    13981 gcagcagcat tcctttcgaa atgatgccga catactgttg gtcctcatcg accacgggca
    14041 ccgcacaggg ggcctgtccg acatgagaga gcaactcgct aagaggcgtt tgtgcatcga
    14101 ccgctaacgg cgcatcaatc agcgccgcat caagaccttg ctgctgcgtt aacgcggctt
    14161 taagcgaatc gatagacaca gcgccgacaa acttattacc gcgttcgatg acgtagccat
    14221 attcgcgatc ttcatcctgc aataatttca gtgccgaacg cgggccgaag ccgggggttt
    14281 tacgaattaa gccgttcggt gtacgacggg caatatcttt cgcactgaac acctggctaa
    14341 tatcgacgcc acggaagaag gtacggacat aatcattcgc cggattattg agaatttcat
    14401 ccggtgtgcc gacctgtact acttcaccat tttgcataat ggcaattcgg tcgccaatac
    14461 gcatggcttc atcgagatcg tgggaaataa agacaatggt gcgctgatgt ttcgcctgta
    14521 attttaccag ctcatcctgc atctcggtgc gaattaatgg atcgagcgcc gagaaggctt
    14581 cgtccattaa taatatatcc ggattaatcg ctaacgcgcg ggctaatccc acacgttgac
    14641 gcatcccgcc agagagttca tccgggtagc tgtgggcata attttccagc ccgacctgac
    14701 gcagtgcatc aagggctttt tcacggcgtt cttcggcatt aattccggcc agttccatac
    14761 cgaacgcagt attgtccagc acggtcatat gcggcattaa ggcaaaggac tggaagacca
    14821 tcgcaatctt ttttctgcgc acctcacgga gttcggcgtc ggatattttg gcaatatcca
    14881 caccatcaat cagcacttgc ccgcgggtgg gttcaatcag gcgattgaga aggcgtacca
    14941 ttgtggattt acccgaaccg gataatccca tgatgacaaa tatctcgcct tcttcaatgg
    15001 ccagactggc gtctttaacg ccaagcgata gcccagtttt ttccagaatt tgttcttttg
    15061 aaagtccttg ttcgatatat ttgaaagctc gctgtggatg ctcgccaaat attttataaa
    15121 gatttttaat ttctaattta attgccatgc aatagaaaga ttcctgttat ttgtttatgt
    15181 cgatacatta cccaatggaa atagtcactt ttttctaccc taacatactg agaatctgag
    15241 gcaacccctg atggcaaatg tggcatgaat attgatgtga gcaaatcgcc ggaatttccc
    15301 atgatataag ggctgagagc aaatcgagaa aagttgatat tttttgtcaa agatagcaaa
    15361 aaaacgcctg aatattgtgg tttcaggcga ataatgcaac agagtatcag tgtagatcac
    15421 cacaaattat ttgcgtgata taaagctatt tggcgggaat tatttccctg cagcgggtag
    15481 tgatattttt gaaaataacc ccctcagaaa ttccagtctt catcttctgt ttcgaccgct
    15541 tttcccatca catatgaaga gcctgaaccg gaaaagaaat cgtgattttc atcggcattc
    15601 ggcgaaagcg cggcgaggat tgccggattc acttccgcca tttctgcggg aaatagcggc
    15661 tcgtagccca gattcatcaa cgccttattg gcgttgtaac agagaaacgc tttcacatcg
    15721 tcagcccacg gggtttcggc gtacagctca tcggtgtatt gcaactcgtt gtcatagagt
    15781 tccagcaaca aatcgaaggc gaaactcttc aattcttcac gtcgtgcctg ggagatcttt
    15841 tccaggtttt tctgatattt atagccgatg tagtaaccgt ggactgcttc atcgcggata
    15901 atcagacgga tcaggtctgc ggtattggtt agctttccgc ggctggaaaa atacatcggc
    15961 agccagaaac cggaatagaa caaaaaagat tcaagaaaca cactggcgat tttctttttc
    16021 agcggatcat caccgcgata atgttgctga ataatctgtg cttttcgctg caacggcgcg
    16081 ttttcttcac tccaggcgta ggcggcatcg acatctttgg tctggcacag cgtcgagaaa
    16141 atcgaactgt aagagcgggc atgaaccgcc tccataaagc tgatattcga taatactgcc
    16201 tcttcatgag gcgtgagcgc atcgggcatc agcgaaggcg cgccgataac attttgcagc
    16261 gtgtcgagca gtgtcaggcc agtaaaaacg cgcatcgtca gttgttgttc tacgacactt
    16321 aatgtctgcc aggcgggaat atcgttcgat agtggcacct tttctggtag ccagaaattg
    16381 ctggtcaggc ggttccacac ctccagatct ttatcgtctg ttatcttgtt ccagttgatg
    16441 gcgctgatac gtgagagttt catagatatt ccttaaagtg cacaggagac gcagccttca
    16501 atttcagtgc cttccagcgc catctgacgc aggcggatgt agtagagcgt tttgatacct
    16561 ttgcgccagg cgtaaatctg cgctttgttg atatcgcgag ttgtggcggt atcggggaaa
    16621 aacagtgtca gcgacagccc ctgatcgaca tggcgagtcg cttcggcgta ggtgtcgatg
    16681 atcttttctg cgcctatttc gtaagcgtcc tgatacagcg ccagattctc gttagtcata
    16741 aacggggcag gatagtaaac gcgtcctgtt ttgccctctt tgcgtatctc tactttcgcc
    16801 acaatcggat gaatactcga cgtggcatgg ttgatataag agatagaacc ggttggcggc
    16861 accgcctgga gattttggtt gtatatgcca tagcgcatca cgtcgtcgcg cagttgcgcc
    16921 cacatttcgc gggtaggtaa cgtgatgctg ctacgagcaa acaactcgcc aactttcgcc
    16981 gttttcggtt gccagttact ttgcagatat tggctaaaat attcaccgct ggcatagcgc
    17041 gactgtttga acccggcaaa ggtttcgccg cgttcgcgcg ccagcaacat cgaggtacgc
    17101 agtgcgtgcc aggtgatggt atagaaatag agattggtga aatcgagtgc ttccggcgat
    17161 ccataagcga tgccttctcg cgccagataa ccgtgtaaat tcatctgccc cagtccgatg
    17221 gcgtgtgagg cggcatttcc ggcttcgatg gacggcacgc tgcggatatg actcatatct
    17281 gacaccgccg ttaaaccgcg cacggcagtc tctaccgtgc gggcaaagtc gggggaatcc
    17341 atggtgtggg caatattcaa cgaacctaaa ttgcaggaaa tatcatggcc tgtgcgggca
    17401 tagtcgagat tctcgtcata ctctgaggcg ctgttaacct gcaaaatttc tgagcagaga
    17461 ttgctcatat ttatgcgccc ggcgataggg ttagcacggt taaccgtgtc ttcatacatg
    17521 atgtacggat agccggactc aaactggatt tctgccagtc gctggaagaa atcacgggcg
    17581 ttgatgtatt ttttgcggat gcgttcattg gcaaccagtt cggcatagtg ttcactgatg
    17641 gcgacatcgg caaatggctt gccataaact cgctctatgt catagggcga aaacagcgcc
    17701 atctgcgcat tctcttttgc cagatggaaa gtgatatccg ggatcaccac gcccagcgac
    17761 agtgttttaa tgcggatttt ttcgtcggca ttttcccgtt ttgtatcgag aaaacgcaga
    17821 atatcgggat gatgagcatg taaatagact gcgccagcac cctgacgcgc gccgagctgg
    17881 ttggcatagg aaaatgcgtc ttccagcatt ttcatcaccg gaatcacgcc agaagattga
    17941 ttttcaatac gtttaattgg cgcgcccgct tcccgcagat tcgacagcaa aaatgcaacg
    18001 ccgccgccgc gtttcgacag ttgcagtgcg gaatttactg cccggccaat cgactccata
    18061 ttgtcttcaa tacgcagcaa aaaacaggaa accagttcgc cgcgctgctg cttaccacag
    18121 ttgaggaatg tcggtgttgc aggctgaaag cgtcctgaca gcatctcatc ggtcagttgc
    18181 gtcgccagtg tttcatcgcc ttgtgccagc gtcagcgcca ccatcgtgac tcgatcggca
    18241 aaatcttcca gataacgctt accgtcgaat gtcttcaacg tatagctggt gtaaaacttc
    18301 catgctccga ggaatgtctg aaaacgaaaa ccgctggcgt gtgcgtgggc aaacagcgta
    18361 atgacaaaat cgctagagta gcgattaagg acactttcat catagtaacc ttcgttaacc
    18421 agccagttca ggcgctgctg ctggctactg aaggccacac tgttcggacg cacatgcgtc
    18481 gtcataaagg cgtctactgc ctggcgatct ttatcgaact gaatgcgacc tgcgctatcg
    18541 taaaggttaa gcatcgcatt cagcgcgtgg taatccatag tttcctgcgt caggcgttct
    18601 gcggttgtcg ttgccaaaat tcggttactc ctttacgaac gttttcgata tcgctttgcg
    18661 tacccatgag ttcaaagcgg tacagccatg gcacgccgca tttccgggca atcacatctc
    18721 cggcgcggcc atatgcctca ccaaagttgc gattacccga agcaataacg ccgcgcagca
    18781 acgcccggtt atgctcgtcg tttaaaaagc gaatgacctg tcgtggcacc gcgccagccg
    18841 taccgccgcc gccgtaagag ggcacgatca ggatgtaagg ctcgtctacc tgaatccgtt
    18901 cccgctcatt gagcgggatg cgcaccgcgg gcagccctaa acgttcgata aaacgctgcg
    18961 tattttcgga gctgctggaa aagtagacaa tctggctcat gcattggccg cgtgtggtgc
    19021 tggatgcaga cggttgatca tgtccgggcg gaaacctgac cagctaagat cgcctgcaat
    19081 cacgaccggc aactgacgaa agccctgagc acgcagcgct tccgccgctt caggaacgcg
    19141 atcgacatta atcatttcaa agtcaaagcc ccggttttcc atcgcccttt tggtggcgtg
    19201 gcactgaacg caatcgttac gagtgtaaat agtaatgcgc atgattcgta tttccgttta
    19261 aaatgaagat acggcgcgat gatacgcgtc gggttgtctc tctgttgata cagaaatact
    19321 agatgtagtt gaaaaaagat tcaaccacac aatatatagc aaatagtggt cgaaattacc
    19381 ctgggtatga gcgtgacggg gtagggggat ttttgtgatt caccaggcaa aaagaaaccc
    19441 cgaagccagg cttcggggtt aaagacgcgt atttattatc atgttgcact acgatttgcg
    19501 catgcttaac agtgcgccga taaaaatacc taccgcagct gctgtaccca cgctacacca
    19561 gggtctttca cgaacaaaag aatccgcgca gccaacggca tcgcgcgcgg cttgctggac
    19621 gcgagtacga ccatgcattc gcgctctggt ttctttcagt agagcctgcg ctttactacg
    19681 agcggcttcg gcttcccctt tggcgtcgct gccccaggac ttcaatacag attcgagact
    19741 atcagctaat tgattgacat cattctggat atcctgcacg ccatcatcaa cgtcgttgcg
    19801 gttcggtcgg ttaaacatat gatcccccat tagaattcga tgtaattttc agtgtagacc
    19861 ataattttct taactgaggt tcatcgcagc tttatggact tttctgaaag cgttgtgaat
    19921 gcgcaatcag gtaaggtagg cccgcggttg ataataacga ggatcaaata tgtatttacg
    19981 accagacgag gtggcgcgcg tacttgaaaa agtcggtttt actgtcgatg tggtaaccca
    20041 gaaggcttat ggttaccgcc gtggggaaaa ttatgtctat gttaatcgcg aagcgcgaat
    20101 gggacgtacc gccctggtga ttcatccaac attaaaagaa cgtagttcaa cactggctga
    20161 acccgcttcc gatattaaaa cttgcgatca ttatcaacaa tttccgctct atttagcagg
    20221 cgagcgacac gagcattacg gtatcccgca tggctttagt tcgcgtgttg cgcttgaacg
    20281 ttatttgaat ggtttatttg gcgaagccag ttaaagacgc gactggcgat gccagtcgcg
    20341 aaaagaaggg tcaggctttt acctgctggt aacggctgac tttaaacagt cggcggcaat
    20401 aatcgaggaa gtagccataa accgccccca tcaacatcga aacaacgatg tttgaactga
    20461 ccgccgccat aatctgatgc cagtctgcgc ccaccactaa caagatcgcc acatacaccg
    20521 gtgactggaa cgtcacataa gccatgatat ccgccagatt ttttatccag cccgacgggc
    20581 taactttgcg tgctgcgcgc ataaacagat cgcggtacat accgtatggc catgcaatta
    20641 agatgtttac cggaatcgct accaatctgg aataaaaaga ctgttcgaag ctcattccgg
    20701 agaggaaaac ttcaatacac atgttcacga cagaacagta aacaaccatc gcgaacgtat
    20761 ctgcaactgc atgacgcaag cgtgactgcg gtgagaacat gctaatgctc cttaataaaa
    20821 gcgtaatggg atgtgaatgg agatgaaagc agtggatctc attgccatct gcatttttgc
    20881 atagcgtata tcgctggcta tgactaatca attagtgaaa aatttttatt taatctgttt
    20941 ttgtagataa aatattccgt agcggcgcgt tttttgtaca ttgtggattt ttacatgccg
    21001 atatgattta tttttaatta aatcaattgg atatgtgttt acctttattt tcact
//
LOCUS       DAZGQL010000088        20879 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.088, whole genome shotgun sequence.
ACCESSION   DAZGQL010000088 DAZGQL010000000
VERSION     DAZGQL010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20879
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.088"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(374..1570)
                     /gene="hemY"
                     /locus_tag="ACHKIR_003786"
     CDS             complement(374..1570)
                     /gene="hemY"
                     /locus_tag="ACHKIR_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0042168 - heme metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HFO9991464.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTAL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1573..2778)
                     /gene="hemX"
                     /locus_tag="ACHKIR_003787"
     CDS             complement(1573..2778)
                     /gene="hemX"
                     /locus_tag="ACHKIR_003787"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HFO9991465.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAVQLE
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPQAD
                     APAAAPQGE"
     gene            complement(2800..3540)
                     /gene="hemD"
                     /locus_tag="ACHKIR_003788"
     CDS             complement(2800..3540)
                     /gene="hemD"
                     /locus_tag="ACHKIR_003788"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HFO9991466.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPTYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CVIHYDGAEEAMRWQSREVTTVVVTSGEMLQQLWSLIPQWYREHWLLRCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3537..4478)
                     /gene="hemC"
                     /locus_tag="ACHKIR_003789"
     CDS             complement(3537..4478)
                     /gene="hemC"
                     /locus_tag="ACHKIR_003789"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HFO9991467.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYESLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            4865..7411
                     /gene="cyaA"
                     /locus_tag="ACHKIR_003790"
     CDS             4865..7411
                     /gene="cyaA"
                     /locus_tag="ACHKIR_003790"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HFO9991468.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRREVLTQLVKEWEWDDARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     CNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            complement(7451..7771)
                     /gene="cyaY"
                     /locus_tag="ACHKIR_003791"
     CDS             complement(7451..7771)
                     /gene="cyaY"
                     /locus_tag="ACHKIR_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HFO9991469.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(7819..9876)
                     /locus_tag="ACHKIR_003792"
     CDS             complement(7819..9876)
                     /locus_tag="ACHKIR_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000611185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9991470.1"
                     /translation="MINTSRQDGFMHFRNVVCLLSCSLFLSSAWGCRLSAPEHNIYQK
                     QGKGVVYLRPYKETNLSLPDVNYKSLRRLPNLLIDPTTLDKWDKVPPLTDLTTDYLYE
                     GAQAWYPHYSWHSDGRYILYAGEVVQNPPDKPPVDVASFKAWGDFAADKHSLYFEGTR
                     TDDNGGENSLDIKTLHQVKFRSPWKPDFLGLILRDANFLYVDGHRLADPESFRVLAQK
                     SWDQRGKFSTTFNPCVAVPFGPWDTLARTRTKIIINGEQLKADPETFTVVRWMPGSLL
                     TWRDKSGLHRKVLNQGNLDKDRANSCAAFTLQEHSVSWRKGPECQQEVLPGLDPEQFH
                     PLSNTVAQYQDKLYTIKKTGFGENRLNIVTLDNPGLVIDKRFNAGRTHGYLLTKLPAD
                     GEEEDGLQVFDSSGPLILMDYRVPDEHEAHLSDSPHYKKWYAHDDRYVYAFDGSQLWR
                     YPTPNTKAVRVKWKTKDSGYGYWANYRSGELDGRLLEDGAFIPTGIPYNSSVNIAQYS
                     DGEGSFLVGETDVSWRKLRSTDQQWSQWEKLPDIDPKAFHPITDRVAQYKNHLYIAKL
                     SPFGEDTLEIITLDSSAPFLKQRINAGKGHGYFMRSSRLRDDIQIFAIDGPLKTTERF
                     AYDNRYVYTWIGSQLYRTASPCPARTRNLDQNMFSSNDDIIILTTEEECHKTPDIEQT
                     LKL"
     gene            complement(10218..10772)
                     /locus_tag="ACHKIR_003793"
     CDS             complement(10218..10772)
                     /locus_tag="ACHKIR_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3304 domain-containing protein"
                     /protein_id="HFO9991471.1"
                     /translation="MGLFTQLEKFDQKLTRGYARWGRWVWRTLIVVPVLVVLWNIGQA
                     VWGGPRGGVILEIHSEIDRPILGFSVNGVAGANAFANGGGSITCCGDVSGDTAEVIWT
                     LSTTRTQYNAGMRLEKRNMTLPLPKREWGENYLHVHFMPGDKVLLGWSKDSFSPYEDL
                     HNGGYKTRVRQDVKDKLYGTGKMN"
     gene            11114..11317
                     /gene="yifL"
                     /locus_tag="ACHKIR_003794"
     CDS             11114..11317
                     /gene="yifL"
                     /locus_tag="ACHKIR_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO9991472.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVEM
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            11354..12178
                     /gene="dapF"
                     /locus_tag="ACHKIR_003795"
     CDS             11354..12178
                     /gene="dapF"
                     /locus_tag="ACHKIR_003795"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFO9991473.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            12175..12882
                     /gene="yigA"
                     /locus_tag="ACHKIR_003796"
     CDS             12175..12882
                     /gene="yigA"
                     /locus_tag="ACHKIR_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HFO9991474.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDCWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            12879..13775
                     /gene="xerC"
                     /locus_tag="ACHKIR_003797"
     CDS             12879..13775
                     /gene="xerC"
                     /locus_tag="ACHKIR_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFO9991475.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDVTMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            13775..14491
                     /gene="yigB"
                     /locus_tag="ACHKIR_003798"
     CDS             13775..14491
                     /gene="yigB"
                     /locus_tag="ACHKIR_003798"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HFO9991476.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            14575..16737
                     /gene="uvrD"
                     /locus_tag="ACHKIR_003799"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             14575..16737
                     /gene="uvrD"
                     /locus_tag="ACHKIR_003799"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HFO9991477.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLETV"
     gene            complement(16782..17684)
                     /locus_tag="ACHKIR_003800"
     CDS             complement(16782..17684)
                     /locus_tag="ACHKIR_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HFO9991478.1"
                     /translation="MKKTLATLFLLTCLGSSSAYADNALILQTDFSLKDGAVSAMKGV
                     AFGVDHNLKIFDLTHEIPPYNIWEGAYRLYQTASYWPQGSVFVSVVDPGVGTDRKSVV
                     LKTKNGQYFVSPDNGTLTLVAQTLGIDSVREIDEKANRLKGSEKSYTFHGRDVYAYTG
                     ARLASGAITFEQVGPELPAKVVELSYQKAKATKGEVKGNIPILDIQYGNVWSNISDEL
                     LKQAGIKLNDTLCVTISEGSQQKYAGKMPYVASFGDVPEGQPMVYLNSLLNVSVALNM
                     DNFAQKHQVASGADWNIDVKKCAK"
     gene            complement(17767..18531)
                     /gene="yigE"
                     /locus_tag="ACHKIR_003801"
     CDS             complement(17767..18531)
                     /gene="yigE"
                     /locus_tag="ACHKIR_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HFO9991479.1"
                     /translation="MAHRLFIGKGMITLNLKRIFLTLTLLPLFAVAADDCAPSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADINSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQKVALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKTSKEIQFAVQSGP
                     LLMENGVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKAKLNVG
                     QLLYLDGTISHMYMKGEAIPWQRYPFVTMISVEQKG"
     gene            18693..18749
                     /gene="ysgD"
                     /locus_tag="ACHKIR_003802"
     CDS             18693..18749
                     /gene="ysgD"
                     /locus_tag="ACHKIR_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsgD"
                     /protein_id="HFO9991480.1"
                     /translation="MDTPSRYWLTILSSRINS"
     gene            18901..19851
                     /gene="corA"
                     /locus_tag="ACHKIR_003803"
     CDS             18901..19851
                     /gene="corA"
                     /locus_tag="ACHKIR_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HFO9991481.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(19887..20384)
                     /locus_tag="ACHKIR_003804"
     CDS             complement(19887..20384)
                     /locus_tag="ACHKIR_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF943 family protein"
                     /protein_id="HFO9991482.1"
                     /translation="MKSKLQKIILCLFLLCCIYNLWTLRPVQILYTYSDAGNSVFLVV
                     DHLPWTDSDKINWYLKHQNEIKNQHPLPEGSWHTWYVIDIGNGFTDYKKYIEGPYEDL
                     YCFPTIKSNDNCIVKNYLMVINEYPYRNTHFFIADNNEYQLTQENKIERVFNPHDFEK
                     DNFQQ"
     gene            complement(20381..>20879)
                     /locus_tag="ACHKIR_003805"
     CDS             complement(20381..>20879)
                     /locus_tag="ACHKIR_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YPO3983 family protein"
                     /protein_id="HFO9991483.1"
                     /translation="FRYGNGNSFHSQELNLSFHERINKYDYNSPIRVIKECIENDISS
                     TPTIGYRPLLLQKIKTELLSSRLNKFNDFEDSFNGLGISVHDISAQKISLLSFQKYAI
                     GWSATIHFVAQDHFGLDVTDIKNKTYSKYRFFRIWFFLQRHKDFAFKPFFTNFNTIER
                     IENYL"
BASE COUNT         4934 a         5341 c         5375 g         5229 t
ORIGIN      
        1 gcgcaggaga aatattcact caggaagtta ttactcagga agcaaagagg attacagaat
       61 tatctcataa caagtgttaa gggatgttat ttcccggttc tctgtggcat aataaacgag
      121 tagatgctca ttccatctct tatgttcgcc ttagtgcctc ataaactccg gaatgacgca
      181 gagccgttta cggtgcttat cgtccactga cagatgtcgc ttctgcctca tcagacacca
      241 tggacacaac gttgagtgaa gcacccactt gttgtcatac agacctgttt taacgcctgc
      301 tccgtaataa gagcaggcgt ttttttatgt atcagaaagg tcccggagga gcttgcctcc
      361 gggtgagaaa gagctactgt ggcgggttgt tctgcaacgt taacatcaaa ccgtcgcgac
      421 gcatcgctgc ggcttcttcc ggcttgtgca gtctgtccag cgcgtcggca agccatgcgt
      481 aatcgtaggc gtccggacgt tgtttcagcg ctgcgcggaa ggcgagcgat gcttcctgcc
      541 attcgccgtg cttcatcagc gactgaccca gtgtgctcca caacagcggg cgatcgccca
      601 cgtttttgat ctgttggcgc agcacttttt caagctgttc cgggttattg gttttcagac
      661 gcgggatcgg cagcagcagg cgatcgtcat actggcgttt caggccgtcg atgataattt
      721 gctgggcagt atcatgatcg tcacattcaa taagatgttc cgccattgcc acctgtaacg
      781 ccacctgatg acgcgttttc cggctttggt ttttccacca gttacgcaaa ccttcactac
      841 cgttatcggc acgcgcctga tccatcaggc caatccatgc ctgttgttcc agcattgcac
      901 gatgttcttc atcaccaaca tgggctttcg ccattgatgg gatgatatcc agcagcgaac
      961 tccatgcacc agtgcggata tacgcctgtt ccgccagacg taatacttcc ggatggcgtg
     1021 gcgtaacttc cagcagctta tcaacgccgt gacgtgctgc atggttttca ttacgggcca
     1081 gttgcagacg tacacgggtg atttctaccg gaatggtgtc attgccggcc agctccgctg
     1141 cgcgttccag atgttggttg gcgcgtgctt catcaccacg ttgttgcgcg gcttcggctg
     1201 ccagtagata gttcaccacc ggttgttcag cgtgatcggc atttttcgcc atcagctttt
     1261 caacttgctg ataatcgcct tccgccagtt tcagcagcgc ctgttcggtc tgcttacgtg
     1321 cacggcgacg cttacgtccg acaaaccacc cacgggtgtg cgcgcctgtg cggaagattc
     1381 gccgcagtag ccactcaatg gcaaacagca ctaccatcgc cagaatcaaa ataatcgcga
     1441 gggccgtgac gctagtttca atattgtagt tgtcggtctg gatcagcacg taaccctgat
     1501 ggccggcaat catcgggcca accacgatcc ccgcaatcag caacacaaag agcaataaca
     1561 cttttagcat ggttattctc cttgcggcgc ggctgccgga gcatcagctt gcggtgcagg
     1621 tgcaggtgca ggtgcaggtg caggtgcagg tttagcttcc gttgtccccg ctgccggttg
     1681 tgccagcagg ttacgcacgc gagtttgcat cagtttttcc agcatcgcct ggctttgcag
     1741 ggtttccgga agatccatcg agatattttg ctggcttaac tggtccacct cgtcgaggaa
     1801 cgctttggtg gtggcatcat cagtatcgta gtaagcacgt acccaggtgg agacgttctc
     1861 cagcgcctgg cgataagtct cttcctggtg acgcggtaca gcttgtgctg cgaccagcag
     1921 gcgagagcga atattttcgc gcagatagat atcctgattt ggcgctaaca gcggtacggc
     1981 ggtgtcatca cgacggcgaa tcgtaatgaa gttgtccata aagttctgcc agcttttttg
     2041 cagattgata cgccattcgc tgatggaact ggaaagctct tcaccgtcgg aatccatcgg
     2101 cgaaccatcg ctgtcattat cagccagacg cagattatct acctgatttg aaagctgatt
     2161 aagcttaagg atgataccgt cataatccac ctgcgatacc gcagaaaggc tggcgatatc
     2221 atcggtaatt gcccgacgaa cggtaatcag actcgggtca ttcatatccg ccaggctggc
     2281 atctgcactt ttcagcaacg ctgcagcggt cgtgacgtct tgatcgctcc acagcttccg
     2341 tcctgcgagt ttcaccagga aatcagcctg agccagcagc caggttttag catcgctgcc
     2401 ggaaatagtc gcgacctttt gttggacttc atccagctgt tttgccagcg tttcttgctg
     2461 acgattcgcc tgctcaagct gtacagcttg ttgcttgata atgccttcca gctcagcttt
     2521 ttggctctcc tgggcttttt gcagtgccgt aagttggtta gccagggcat cgctggtggc
     2581 agtctgattg acggcctgtt gtttacccca gccatacaaa ccgacgcccg ccgccagagc
     2641 aatagcgata gccaccgcgc tgagaatcaa tgcggtattg ttcttactct ttttttctgt
     2701 tgcgacaggt tgtgacgtgg tgtccacggc ctccctggtc tcttcaacca cggcggaggt
     2761 tttttcttgt tccgtcatta tggcttcctg ttatgagagt tattgtaatg cccgtaaaag
     2821 cgcatcgttg tcagcgttat cggcgacctt aatgtcttgc cagcccagtt cccgggcgag
     2881 tttcgccaaa cgctcactga cgaccaatag tcgacagcgt agtaaccagt gctcacgata
     2941 ccattgtggg atcagcgacc agagttgctg caacatttca ccgctggtaa caacgaccgt
     3001 cgtcacctcg cgggattgcc agcgcatcgc ttcttctgca ccatcgtaat ggattacgca
     3061 tcgttgataa cattcacaaa aagtgacctc agcaccgcgc gccgtcaggg tatccccaat
     3121 tagctcacga ccgccattgc cacgtaatat cagcgcacgt ttgcccgcaa tattttgtaa
     3181 ttcaggtaat tgtagcaaga cttcgctgat ttcccgatcc tgcgggtaga gaatcttctg
     3241 tccgcttacg gtatgtagtg ccagtgcggt ggtgcgtcca atggcgaaat aagtaggtag
     3301 tcggggccat ttacgatctt gctgatgcag ctgtgattgg gcaaaagcaa ccgcgtgttg
     3361 cgagagggca aacaacagat cgctctcccc cagcgccgcc agttgatcag caagttgtgg
     3421 taattgtcga cccggagaaa actcaatcag tggaaaatgc caggccacct gccccagtgt
     3481 gcgcagacgg ctcactaact cttctccagc gggagacggg cgggtgacaa ggatactcat
     3541 gccggggcgt ctccattata gacttcagcg aggatctcgc gcgcgccgtt attcagtagc
     3601 tcttctgcca gcgaaatccc catttgttcg gcatcttgcg gcgcaccgcg gcgttcaccg
     3661 cgaataatct gcgaaccgtc cggcgcgccg accagcgcac gcagccagat ttcaccatca
     3721 ataagctcgg cgtagctacc aattggcacc tgacatccgc cttcgagacg ggtattcatg
     3781 gcgcgttctg cggtaacgcg cagtgcagtt tcgtggtgat tcagcgcggc aagcagctcg
     3841 cgagtgcgtg tatcatcaag gcggcattca ataccgaccg caccttgtcc taccgccgga
     3901 agagaaatct cgggtggtaa cgcggcgcga atacgtgact ccagacctaa acgttttagt
     3961 ccggctacgg caagaatgat ggcatcgtat tcgccgttat ccagtttact caggcgagtg
     4021 ccgacgttgc cgcgcaggga gcggataatc agatccgggc ggcgttcagc cagttggcac
     4081 tggcgacgta aactggacgt cccgacgata ctgcctgccg gtaacgcatc cagactttca
     4141 tagttattgg acacaaaggc atcgcgagga tcttcacgct cgcaaatggt gaccagcccc
     4201 agaccttgcg ggaattcaac cggcacatct ttcattgaat gtacggcgat atcggcgcga
     4261 ttctccagga gcgcgacttc cagctcttta acaaataagc cttttccgcc tactttcgcc
     4321 agcggcgtat caagaatcac atcgccgcgc gtcaccatcg gtaccagttc aacgaccagg
     4381 cccggatggc tcgccatcaa cttgtctttg acatagtgtg cctgccagag tgcaagtggg
     4441 ctttggcgtg tggcaattct taaaacattg tctaacatgc ttgttaccgt cattatcatc
     4501 cgtggtccat cctaacatcc ttgccagagc gatgtcagtg ttgtggtgaa acgtagacgc
     4561 ctgcgcaaac cgtaaaatga ggtctggcag tggatcctga caggcgtttc acgccgtcgt
     4621 aataaggaat ttacagagaa taaacggtgc tacacttgta tgtagcgcat ctttctttac
     4681 ggtcaatcag caaggtgtta aattgatcac gttttagacc attttttcgt cgtgaaacta
     4741 aaaaaaccag gcgcgaaaag tggtaacggt tacctttgac atacgaaata tcccgaatgc
     4801 cgcgtgttac cgttgatgtt ggcggaatca cagtcatgac gggtagcaaa tcaggcgata
     4861 cgtcttgtac ctctatattg agactctgaa acagagactg gatgccataa atcaattgcg
     4921 tgtggatcgc gcgcttgctg ctatggggcc tgcattccaa caggtctaca gtctactgcc
     4981 gacattgttg cactatcacc atccgctaat gccgggttac cttgatggta acgttcccaa
     5041 aggcatttgc ctttacacgc ctgatgaaac tcaacgccac tacctgaacg agcttgaact
     5101 gtatcgtgga atgtcagtac aggatccgcc gaaaggtgag cttccaatta ctggtgtata
     5161 caccatgggc agcacctcgt ccgtagggca aagttgttcc tctgacctgg atatctgggt
     5221 ctgtcatcaa tcctggctcg atagcgaaga gcgccagtta ctacaacgta aatgcagcct
     5281 gctggaaagc tgggccgcct cgctgggcgt ggaagttagc ttcttcctga ttgatgaaaa
     5341 tcgcttccgt cataatgaaa gcggcagcct ggggggcgaa gattgtggct ccacccagca
     5401 tatactgctg cttgacgaat tttaccgtac cgccgtgcgt ctcgctggta agcgtattct
     5461 gtggaatatg gtgccgtgcg acgaagaaga gcattacgac gactatgtga tgacgcttta
     5521 cgcgcagggc gtgctgacgc caaatgaatg gctggatctc ggtggcttaa gctcgctttc
     5581 tgctgaagag tactttggtg ccagcctttg gcagctctac aagagtatcg attccccata
     5641 caaagcggtg ctgaaaacac tgctgctgga agcctattcc tgggaatacc cgaacccacg
     5701 tctgctggcg aaagatatca aacagcgttt gcacgacggc gagattgtgt cgtttggcct
     5761 cgatccttac tgcatgatgc tggagcgtgt tactgaatac ctgacggcga ttgaagattt
     5821 cactcgtctg gatttagtac gccgctgctt ctatttaaaa gtgtgcgaaa aactcagccg
     5881 tgaacgcgcc tgcgtaggct ggcgtcgtga agtgctaacc cagttagtga aagagtggga
     5941 atgggacgat gctcgtctgg caatgctcga taaccgcgct aactggaaga ttgatcaggt
     6001 acgtgaggcg cacaacgagt tgctcgacgc gatgatgcag agctaccgta atctgattcg
     6061 ctttgcgcgc cgcaataacc ttagcgtctc cgccagccca caggatattg gcgtactgac
     6121 gcgtaaactg tatgccgcgt ttgaagcgtt accgggtaaa gtgacgctgg taaacccgca
     6181 gatttcgccc gatctctcgg aaccgaatct gacctttatt tatgtgccgc cgggccgggc
     6241 taaccgttca ggttggtatc tgtataaccg cgcgccaaat attgagtcga tcatcagcca
     6301 tcagccgctg gaatataacc gttacctgaa taaactggtg gcgtgggcat ggtttaacgg
     6361 cctgctgacc tcgcgtaccc gcttgtatat taaaggcaac ggcattgtcg atttgcctaa
     6421 gttgcaggag atggtcgccg atgtgtcaca ccatttcccg ctgcgcctgc ctgcaccgac
     6481 gccaaaagcg ctctacagcc cgtgtgaaat tcgccatctg gcgattatcg ttaacctgga
     6541 atatgacccg acagctgcgt tctgcaatca ggtggtgcat tttgatttcc gtaagctgga
     6601 cgtcttcagc tttggcgaga atcaaaattg cctggtaggt agcgtggacc tgctgtaccg
     6661 caactcgtgg aacgaagtgc gtacgctgca cttcaacggc gagcagtcga tgatcgaagc
     6721 cctgaaaacc attctcggta aaatgcatca ggacgccgca ccgccagata gcgtggaagt
     6781 cttctgctac agccagcatc tgcgcggatt aatccgtact cgcgtgcagc aactggtttc
     6841 tgagtgcatt gaactgcgtc tttccagcac ccgccaggaa accgggcgct tcaaggcgct
     6901 gcgcgtttct ggtcaaacct ggggcttatt cttcgaacgc ctgaatgtat cggtacagaa
     6961 actagaaaat gccatcgagt tttatggcgc tatttcgcat aacaaactgc acggcctgtc
     7021 agtgcaggtt gaaaccaatc acgtcaaatt accggcggtg gtggacggct ttgccagcga
     7081 agggatcatc cagttcttct tcgaagaaac gcaagacgag aatggcttta atatctacat
     7141 tctcgacgaa agcaaccggg ttgaggtgta tcaccactgc gaaggcagca aagaggagct
     7201 ggtgcgtgac gtcagtcgct tctactcgtc atcgcacgac cgttttacct acggctcaag
     7261 cttcatcaac ttcaacctgc cgcagttcta tcagattgtg aaggttgatg gtcgtgaaca
     7321 ggtgattccg ttccgcacca aatcgattgg caacatgccg cctgccaatc aggatcacga
     7381 cacgccgcta ttacagcagt atttttcgtg atgagcgtgc cggaaagcga ggcttatccg
     7441 gcatgcaatc ttagcggaaa ctgactgttt cacccgcttg ctgcgttgcc gcctgttcca
     7501 gcaaatccca gaaggtttcg ccgctacgat cgcaaatcca ctcatcgcct ttcagatcaa
     7561 aatggtagcc gccctgtttg gttgccagcc atacctggtg cagcggttcc tggcggttga
     7621 taatgatttt gctcccgttt tcaaaggtaa tggtcagtac accgccgttg atttcgcagt
     7681 cgatatcgct atcgccatcc cagtcgtcca gacgctcttc aatggtcagc cacagttgat
     7741 cagccaggcg atgaaattca ctgtcgttca ttgttgtatc ctgtttttaa gtgatggcgg
     7801 cagtatagcg gcatggggtc agagcttcaa agtttgctca atgtctggtg ttttgtggca
     7861 ctcttcctcc gtcgtcaaga tgatgatgtc atcattggat gaaaacatgt tctgatccag
     7921 attacgtgtc ctggccggac agggcgatgc ggttcgataa agttggctac cgatccaggt
     7981 ataaacatag cgattgtcat aagcgaaacg ttcggtggtt ttcaatggtc catcaatagc
     8041 aaaaatttga atatcatcgc gtaaacggga agagcgcata aagtaaccat ggcctttccc
     8101 ggcgttaatc cgttgtttca ggaaaggtgc agaagagtcg agcgttatta tttccagggt
     8161 gtcttcacca aaaggagaca atttcgcaat gtacagatga tttttgtact gcgcgacacg
     8221 atcggtgatg ggatgaaatg ctttcgggtc aatatcgggg agtttctccc attgactcca
     8281 ttgctggtct gtagagcgaa gcttacgcca gctaacatcg gtttcgccga cgagaaaaga
     8341 gccttcgcca tcagaatatt gagcaatgtt aacggatgag ttatagggga ttcccgttgg
     8401 aataaacgca ccatcctcaa gaagtctgcc gtcaagctca ccactacggt agtttgccca
     8461 atagccatat cctgaatctt tggttttcca tttcacccgc acggctttgg tattgggagt
     8521 tgggtagcgc caaagctgtg agccatcgaa tgcgtaaaca taacgatcgt cgtgtgcata
     8581 ccatttcttg taatgggggc tatcgctaag gtgagcctca tgctcgtcag gaacacgata
     8641 atccatcagg ataagcggcc cggaagaatc gaatacctgg aggccatctt cttcttcacc
     8701 atcagctggt aatttggtca ataaatagcc gtgagttctt cccgcgttaa agcgtttatc
     8761 tatcaccaaa ccaggattat ccagtgtgac gatatttagc ctattttcgc caaatcccgt
     8821 ttttttgatg gtgtacagct tgtcctgata ctgagcaacg gtattactaa gaggatgaaa
     8881 ttgttctgga tcgaggccgg gtaggacttc ttgctggcat tctggccctt tgcgccatga
     8941 aacagagtgt tcctgcaaag tgaacgccgc gcagctattt gccctatctt tgtccagatt
     9001 tccctgattc agcaccttcc ggtgcaggcc gcttttatct cgccaggtta ataacgaacc
     9061 gggcatccag cgtacgacag taaatgtctc tgggtcggct ttaagctgtt cgccattgat
     9121 tatgattttg gtccgtgtac gggctaacgt atcccagggg ccaaatggta cggcaacgca
     9181 ggggttaaac gtggtggaaa actttcctcg ctgatcccag gacttctgcg ccagcacacg
     9241 gaaactctcg gggtcagcga ggcgatgtcc atcgacatat aagaaattag cgtcacgtaa
     9301 tatcagcccc agaaagtcag gtttccaggg ggaacggaat ttcacctgat gcagcgtttt
     9361 aatatctaga ctattttctc cgccattgtc atcggtgcgc gtgccttcaa aatagagact
     9421 gtgtttatct gcggcaaaat caccccatgc cttgaacgag gcgacatcga cgggaggttt
     9481 gtctggcggg ttttgcacaa cttcacccgc gtagaggata tagcgaccat cgctatgcca
     9541 ggagtaatga ggataccagg cctgtgcgcc ttcatagagg tagtcggttg tcagatcagt
     9601 cagaggaggt actttatccc atttgtctag tgttgtcggg tctattaata aattgggtaa
     9661 acggcgcaaa cttttgtaat tcacgtcagg aagagaaaga tttgtttctt tataagggcg
     9721 caggtatacc acgccttttc cctgtttctg gtaaatatta tgttcaggcg cggaaagccg
     9781 acatccccat gccgacgata aaaacaacga acaagacaac aaacaaacca cattgcgaaa
     9841 gtgcataaag ccatcctggc gcgaggtgtt gatcacgaaa ctgctagcag aacataaatc
     9901 ctcacaagta agcgtaactc tcgcagcaaa tcgtcacttt tcagaatcca taaaggtcat
     9961 cgctattttc tgatagttgc tggaataaac gtgatatttt cattcacgat ataaacacaa
    10021 tcccggaact gccccatagt acggctaata gcataaaaat attccggatt gacccctgga
    10081 agggaggcaa tgtgggattc cgccccccgt tgccttcttt attgtctttc acgagttgta
    10141 cccaggttcc accacgggta tctaacattc gccacattat tacgctttct gccgaacgtg
    10201 atttttatag aaatctatca attcattttc cctgtaccat aaagtttatc cttcacatcc
    10261 tgccgaacgc gggtcttgta ccccccgttg tgaagatctt catacggaga aaaagaatct
    10321 ttactccacc ccagcaacac tttatcgcca ggcataaaat gcacgtgcag gtaattttct
    10381 ccccattctc tttttgggag aggtaacgtc atattgcgtt tctccagacg cattccagca
    10441 ttatactgtg tacgagtagt acttaatgtc catataacct ctgccgtatc cccactgaca
    10501 tcaccgcaac aggtgattga accgccacca ttcgcaaaag cattcgcgcc cgccacgccg
    10561 ttaacactga atcccagtat cggacggtca atttcactgt ggatttccag tatcactcca
    10621 cctcttggtc ctccccatac tgcctgtcct atattccaca acacgaccag tactggaacg
    10681 acaattaacg tccgccatac ccagcgcccc cagcgggcat aaccgcgcgt aagcttttgg
    10741 tcaaattttt ccagttgggt aaatagcccc atcgatttta ttccctcttc tcacctggtt
    10801 ggcaaagttg cacgtcactg gcgactaagg tcagacaacg aaatttcgtg gtacgccact
    10861 ctcttaaaag tcccatgcag gcgataagaa caagacaaca aacaaaccac attgcgatag
    10921 tgcataaagc catcctggcg cgaggtgccg atcacgaaac taccagcaaa acataaatcg
    10981 ccacgtgtaa gcgttatact cgcagcattt cctcactttt cagacttcat aaagagtcgc
    11041 taaacgcttg cttttacgcc ttctcctgcg atgatagaaa gcagaaagcg atgaacttta
    11101 caggcaatcc ataatgaaaa acgtgtttaa ggcactcact gtattactta ctctcttcag
    11161 cctgacgggc tgcggtctga aaggtccgct ctatttcccg cctgcagata aaaacgcacc
    11221 gccgccgacc aaaccggtag agatgcaaac gcaatccacg gtgccggata aaaacgaccg
    11281 cgccactggc gatggtccat cccaggtgaa ttactaaaag tcagtttctg tacccgcgtg
    11341 attggagtaa atgatgcagt tctcgaaaat gcatggcctt ggcaacgatt ttatggtcgt
    11401 cgacgcggta acgcagaatg tctttttttc accggagctg attcgtcgcc tggcggatcg
    11461 gcacctgggg gtagggtttg accagctgct ggtggttgag ccgccgtatg atcctgaact
    11521 ggattttcac tatcgcattt tcaatgctga tggcagtgaa gtggcgcagt gcggcaacgg
    11581 tgcgcgctgc tttgcccgtt ttgtgcgcct gaaagggctg actaataagc gcgatatccg
    11641 cgtcagcacc gccaacgggc ggatggttct gaccgtcacc gatgatgatc tggtccgcgt
    11701 aaatatgggc gaacccaact tcgaaccttc cgccgtgccg tttcgcgcta acaaagcgga
    11761 aaagacctat attatgcgcg ccgccgagca gacaatctta tgcggcgtgg tgtcgatggg
    11821 aaatccgcat tgcgtgattc aggtcgatga tgtcgatacc gcggcggtag aaacgcttgg
    11881 tcctgttctg gaaagccacg aacgtttccc ggagcgcgcc aatatcggtt ttatgcaagt
    11941 ggttaagcgc gagcatattc gtttacgcgt ttatgagcgt ggggcaggag aaacccaggc
    12001 ctgcggcagc ggcgcgtgtg cggcggttgc agtagggatt cagcaaggtt tgctggccga
    12061 agaagtacgc gtggaactcc ccggcggtcg tcttgatatc gcctggaaag gtccgggtca
    12121 cccgttatat atgactggcc cggcggtaca tgtctacgac ggatttattc atctatgaag
    12181 caaccagggg aagaactgca ggaaacactc acggagcttg atgaccgggc ggttgtcgat
    12241 tatctgatta aaaatcctga gttttttatc cgcaatgcgc gcgcagtaga agcgatacgt
    12301 gtgccgcatc cggtacgcgg caccgtttcg ttggtcgagt ggcacatggc ccgcgcacgt
    12361 aatcacattc atgttctgga agagaacatg gcgttgttga tggagcaggc tatcgccaac
    12421 gaaggcctgt tttatcgctt actctacttg cagcgtagtc tcaccgccgc cagcagtctc
    12481 gacgatatgc tgatgcgctt tcaccgctgg gcgcgcgatc tcggcctggc gggtgcgagc
    12541 ctgcgcctgt tcccggattg ctggcgttta ggtgcgccgt cgaaccacac tcatctggca
    12601 ttaagccgtc agtctttcga accgctgcgt attcagcgtt tggggcagga acagcactat
    12661 cttgggccgc ttaacggacc ggagctgctg gtggtgctac cggaagcgaa agcggtggga
    12721 tcggtggcga tgtcgatgct gggaagcgat gctgatttgg gtgtcgtgct gtttaccagt
    12781 cgcgatgcca gtcactatca acaagggcag ggaacgcagt tacttcatga aattgcgctg
    12841 atgttgccgg agcttctgga gcgttggatt gaacgcgtat gaccgattta cacaccgatg
    12901 tagaacgcta cctacgttat ctgagcgtgg agcgtcagct tagcccgata accctactta
    12961 actatcagcg tcagcttgag gcgatcatca attttgccag tgaaaacggc ctgcaaagct
    13021 ggcagcaatg tgatgtgacg atggtgcgca atttcgctgt acgcagccgc cgtaaagggc
    13081 tgggagctgc aagtctggcg ttacggcttt ctgcgctacg tagctttttt gactggctgg
    13141 tcagccagaa cgaactcaaa gccaacccgg cgaaaggtgt ttcggcaccg aaagcgccgc
    13201 gtcatctgcc gaaaaacatc gacgtcgatg atatgaatcg gctgctggat attgatatca
    13261 acgatcccct cgccgtgcgc gatcgggcaa tgctggaagt gatgtacggc gcgggtctgc
    13321 gtctttccga actggtgggg ctcgatatca aacacctcga cctggagtcc ggcgaagtgt
    13381 gggtgatggg aaaaggcagc aaagagcgcc gcctgccgat tggtcgcaac gctgtggcgt
    13441 ggattgagca ctggcttgat ttgcgcgacc tgtttggtag cgaagacgac gcgctttttc
    13501 tgtcgaaact gggcaagcgt atctccgcgc gtaatgtgca gaaacgcttt gccgaatggg
    13561 gcatcaaaca agggctgaat aatcacgttc acccgcataa attacgtcac tcgttcgcta
    13621 cgcatatgct ggagtcgagc ggcgatcttc gtggtgtgca ggagctgctg ggtcatgcca
    13681 acctctccac cacgcaaatc tatactcatc ttgattttca acaccttgcc tcggtgtacg
    13741 atgcggcgca tccacgcgcc aaacggggga aataatgcgt ttttaccggc ctttggggcg
    13801 catctcggcg ctcacctttg acctggatga taccctttac gataaccgtc cggtgatttt
    13861 gcgcaccgag cgagaggcgc ttacctttgt gcaaaattat catccggcgc tgcgcagctt
    13921 ccagaatgaa gatctgcaac gcctgcgcca ggcggtacgg gaagcggaac ccgagattta
    13981 tcacgacgtg acgcgctggc gttttcgttc gattgaacaa gccatgctcg acgccgggct
    14041 gagtgccgaa gaagccagtg caggcgcaca cgcagcaatg atcaactttg ccaaatggcg
    14101 cagccgaatc gatgtcccgc agcaaaccca cgacacctta aaacagctgg cgaagaaatg
    14161 gccgctggtg gcgatcacca acggtaacgc ccagccggag ctgtttggtt taggggatta
    14221 ttttgagttt gtgttgcgcg ctggcccgca cgggcgctca aaaccgttca gcgatatgta
    14281 cttcctggcg gcggaaaaac tcaacgtgcc gatcggcgag atcttacatg ttggggacga
    14341 tctcaccact gacgtgggtg gggcaattcg cagcggaatg caggcctgtt ggatcagacc
    14401 ggaaaatggc gatctgatgc aaacctggga cagccgttta ctgccgcatc tggaaatttc
    14461 ccggttggca tctctgacct cgctgatata atcagcaaat ctgtatatat acccagcttt
    14521 ttggcggagg gcgttgcgct tctccgccca acctattttt acgcggcggt gccaatggac
    14581 gtttcttacc tgctcgacag ccttaatgac aaacagcgcg aagcggtggc cgcgccacgc
    14641 agcaaccttc tggtgctggc gggcgcgggc agtggtaaga cgcgcgtact ggtgcatcgt
    14701 atcgcctggc tgatgagcgt ggaaaactgc tcgccatact cgattatggc ggtgacgttt
    14761 accaacaaag cggcggcgga gatgcgtcat cgtatcgggc aactgatggg caccagccag
    14821 ggcggcatgt gggtcggcac cttccacggg ctggcgcacc gtctgctgcg tgcgcaccat
    14881 atggacgcca atctgccgca ggattttcag atcctcgaca gcgaggacca gctacgcctg
    14941 ctcaaacgcc taatcaaggc gatgaacctc gacgagaagc agtggccgcc gcgccaggca
    15001 atgtggtaca tcaacagcca gaaagatgaa ggcctgcgtc cacatcatat tcaaagctac
    15061 ggtaatccgg tggagcagac ctggcagaag gtgtatcagg cgtatcagga agcgtgtgac
    15121 cgcgcgggcc tggtggactt cgccgagctg ctgctacgtg ctcacgagtt gtggcttaac
    15181 aagccgcata tccttcagca ctatcgcgaa cggtttacca atatcctggt ggatgaattc
    15241 caggatacca acaacattca gtatgcgtgg atccgcctgc tggcgggcga taccggcaaa
    15301 gtgatgatcg ttggcgatga cgaccagtca atctacggct ggcgtggggc gcaggtcgag
    15361 aatattcagc gtttcctcaa tgatttcccc ggtgctgaaa ctatccgtct ggagcagaac
    15421 taccgctcta ccagcaatat tctgagcgcc gctaacgccc tgattgaaaa caataacggg
    15481 cgtctgggta aaaaactgtg gaccgatggc gcggacggtg agcctatttc cctctattgc
    15541 gcttttaacg aactcgacga agcgcgtttt gtggttaacc gtatcaagac ctggcaggac
    15601 aatggcgggg cgcttgccga gtgcgccatt ctctaccgca gcaacgccca gtcgcgtgta
    15661 ctggaagaag cactgttgca ggcgagtatg ccgtaccgta tttacggcgg gatgcgcttc
    15721 ttcgaacgcc aggaaatcaa agatgcgctc tcgtatctgc gcctgattgc caaccgtaat
    15781 gacgacgcgg cgtttgagcg cgtggtgaat acaccaacgc ggggtattgg tgaccggacg
    15841 ctggacgtgg tacgtcagac atcgcgcgat cgccagttaa cactctggca ggcatgtcgt
    15901 gaactgttgc aggaaaaagc cctcgccgga cgtgctgcca gtgccttaca gcggtttatg
    15961 gaactgatcg acgccttagc gcaggaaact gccgatatgc cgctgcatgt acagactgac
    16021 cgggtaatta aagactccgg cctgcgcaca atgtacgagc aggagaaagg cgaaaaaggc
    16081 cagacgcgta tcgaaaactt agaggaactg gtaacggcga cgcgccagtt cagctacaac
    16141 gaagaagacg aagatttaat gccgctgcag gcgttcctct cccatgcggc actggaagcg
    16201 ggcgaggggc aggcggatac ctggcaggat gcggtgcagt tgatgacgct acactcggcg
    16261 aaaggtctgg aattcccgca ggtgtttatc gttggtatgg aagagggcat gttcccaagc
    16321 cagatgtcgc tggatgaagg cgggcgtctg gaagaagaac gccgtctggc ctacgttggc
    16381 gtaacccgcg cgatgcagaa actgacgctg acctacgcgg aaacccgccg tctatatggc
    16441 aaagaggttt accatcgccc gtcgcgcttt atcggtgagt tgccggaaga gtgtgtggaa
    16501 gaggtgcgcc tgcgcgccac ggtaagccgc ccggtcagcc atcaacggat ggggacgccg
    16561 atggtcgaga acgacagcgg ctacaagctc ggccagcgcg tacgccacgc taagtttggt
    16621 gaaggcacca tcgtcaatat ggaaggcagc ggtgagcata gccgtttgca ggtggcgttc
    16681 cagggccagg gtattaaatg gctggtggcg gcttacgccc ggttggagac ggtgtgacgt
    16741 tgcgggatgc ggtgcgccac accgcattcc gcatcagcac cttacttagc gcacttcttc
    16801 acatcaatat tccagtcagc accagacgca acctgatgtt tctgcgcaaa attatccata
    16861 ttcagcgcca cggaaacatt caataagctg ttcagataca ccatcggctg gccttccggc
    16921 acatcgccga agcttgcgac atacggcatt ttccctgcgt atttttgctg ggaaccttcg
    16981 gaaatggtca cacacaacgt gtcgttgagt ttgattcctg cctgtttcag caactcgtcg
    17041 ctgatattgc tccagacgtt gccgtactga atatcgagaa tcgggatatt gcccttcact
    17101 tcaccttttg ttgcttttgc tttctgataa gaaagttcga caacttttgc aggaagctct
    17161 ggccccacct gttcgaacgt aatcgctccg gaagccagac gcgcaccagt gtaggcatat
    17221 acatcgcgac catggaaggt atatgacttt tcagaacctt tcaggcggtt agctttttcg
    17281 tcgatttccc ggacgctatc aatacccaga gtttgtgcca ccaaagtgag cgtaccgtta
    17341 tctggtgaaa cgaagtattg accgtttttg gttttcagca ctaccgattt acggtcagtg
    17401 cctacgcccg gatcgaccac gctaacaaac accgaacctt gcggccagta actggcagtc
    17461 tgatacaggc gataagcgcc ttcccagatg ttatacggtg gaatttcgtg tgttaaatca
    17521 aagattttaa gattatggtc aacgccgaat gcgacccctt tcatcgccga gacggcacca
    17581 tctttcaggc taaaatccgt ttgtaaaatc aaagcgttat ccgcataagc ggatgatgac
    17641 cccagacagg ttaataaaaa taatgttgcg agcgtttttt tcatttttgt ttcccttcat
    17701 ttatctgatg attttccagc tgtgtagcca gccccagttt gtctggcctc ctcgagtgta
    17761 caatgtttaa cctttctgct cgacggaaat catggtgaca aaaggatagc gttgccacgg
    17821 gattgcctcg cctttcatat acatatgtga aattgtgcca tcaagataaa gcagctgccc
    17881 aacgttcagt ttcgctttgg cataacaggc aaaatcataa aaatttgtcg cctgctggct
    17941 caacaaaaac acggcgttcc catgtttatt aatcccaaca ccgttacgaa ttttacgtga
    18001 ggcgacgttg ggatgaatac gcggattaat tacaccgttt tccatcaaca atggccctga
    18061 ctgcaccgca aactgaatct ctttactggt tttaaaggca tccagacgaa cgatgccgac
    18121 tttatctccc gcgacataaa acactccgcc aggacgaata aagaaattcc cttcacctga
    18181 agcgagattt aacgccacct tctgctgacc gttttcgatg tacaaaccga gcggcgcata
    18241 gctttcatca tagatgccgc cgttcatcgc catctgcacc tgaccctgac tattaatatc
    18301 cgccagcaga gcatgtaacg ttccccacgc ttcgccattg gctttttgcc agtacatttt
    18361 cacccgctct gtttgaggat taacggtata cgcctgtacg gtcagcgtcg gatctgaggg
    18421 tgcgcaatca tcagcggcaa cagcaaacaa gggaagtaag gtgagggtga gaaaaatccg
    18481 tttgagattc aaggtgatca ttcctttacc aatgaatagc cgatgcgcca ttataggccc
    18541 tggatgtggg atttttttat cctgttagcg accttgacga gtaccaaaaa gcgcgaagtt
    18601 caactattgt tctgtggtgt tctgttgcgt gttgacggca aaattttgct ggcgtaacat
    18661 gcgcgcacga tcactctaag aggacattcg ccttggacac acccagtaga tactggctca
    18721 ctatcctgtc atccaggatc aactcctaag gctatccctt tttgctgata gccttagcgg
    18781 ttgtcagcga cctcaatttt tcccgtcgcg ctgagtcagg ctgtttaatg gtctgaaacc
    18841 caatttgttt ctgtgtgccc accgaactgt ccgatatttt aagcattggg agtcccggtc
    18901 atgctgagcg catttcaact ggaaaataac cgactgaccc ggctggaagt cgaagagtca
    18961 caaccccttg taaatgcagt atggattgat cttgtcgaac cggacgacga cgagcgactg
    19021 cgcgtacaat ctgaacttgg tcagagcctg gcaacccgcc cggaactgga agacatcgaa
    19081 gcatcggcac gtttctttga agacgatgac ggcctgcata ttcactcctt cttcttcttt
    19141 gaagatgcgg aagatcacgc cggtaactct actgtggcat ttaccatccg tgatggtcgt
    19201 ctgtttactc tgcgtgagcg tgaactgcct gcattccgtc tgtatcgtat gcgcgcccgt
    19261 agccagtcaa tggtggacgg taacgcctac gagctgctgt tggatctgtt cgaaaccaaa
    19321 atcgaacagt tggcggatga aattgaaaac atctacagcg atctggagca gttgagccgg
    19381 gtgattatgg aagggcatca gggcgatgag tacgacgagg cgctctccac gctggcggaa
    19441 ctggaagata tcggctggaa agttcgcctg tgtctgatgg atacccagcg tgcgctcaac
    19501 ttcctggtac gtaaagcacg cttaccgggt gggcaactgg agcaggcgcg tgaaatcctg
    19561 cgagatatcg aatccctgct gccgcataac gaatccctgt tccagaaggt gaacttcctg
    19621 atgcaggcgg cgatgggctt tatcaatatc gagcagaacc gcatcatcaa aatcttctcg
    19681 gtggtatccg tggtgttcct gccaccgacg ctggtcgctt ccagctacgg gatgaacttt
    19741 gagtttatgc cggaactgaa gtggagcttc ggctacccag gcgcgattat ctttatgatc
    19801 ctcgcgggcc tggcaccgta tctgtacttt aagcggaaga actggttgta atataaatgc
    19861 cgggtaaaga ttgttcttta tccggcttat tgctgaaaat tgtctttttc aaagtcatgc
    19921 gggttgaaca ctcgctctat cttattttcc tgggttagtt gatactcatt gttatcagca
    19981 ataaaaaaat gagtattacg gtaaggatat tcattaataa ccattaaata gttttttacg
    20041 atgcaattat cattgctctt aatcgtcggg aaacagtaga gatcttcata cggcccttcg
    20101 atatattttt tatagtcagt gaagccatta ccgatatcaa taacatacca ggtatgccaa
    20161 ctaccttctg gtaggggatg ttgattttta atttcattct gatgcttcag ataccaattt
    20221 attttatcgc tgtcagtcca tggcagatga tcaacgacca gaaagactga attgccagca
    20281 tcagagtatg tatacaaaat ctgtacagga cgcaatgtcc ataaattata gatgcagcat
    20341 agaagaaata aacataatat aattttctgg agtttgcttt tcatagataa ttttcgattc
    20401 tttctatagt gttaaaattg gtgaaaaaag gtttgaaagc aaagtcttta tgccgttgta
    20461 aaaagaacca tatgcgaaaa aatctatatt tgctataagt cttgtttttg atatcagtta
    20521 catcaagacc gaagtggtcc tgtgctacaa aatgaattgt tgcactccat cctatagcgt
    20581 atttttgaaa acttaaaaga gatatttttt gagctgaaat atcatgaaca ctgattccta
    20641 agccattaaa actgtcctcg aagtcattaa atttatttag tcttgatgaa agtaattcgg
    20701 tcttaatctt ttgtaggaga agaggccggt atcctatcgt aggtgttgaa gaaatatcat
    20761 tttcgataca ttctttgatt acacgtattg ggctattata atcatatttg tttattcgtt
    20821 catgaaaaga taagttcaat tcctggctat ggaaactgtt gccatttcca tatctaaaa
//
LOCUS       DAZGQL010000089        19633 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.089, whole genome shotgun sequence.
ACCESSION   DAZGQL010000089 DAZGQL010000000
VERSION     DAZGQL010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19633
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.089"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..1348
                     /gene="cdgI"
                     /locus_tag="ACHKIR_003806"
     CDS             <1..1348
                     /gene="cdgI"
                     /locus_tag="ACHKIR_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFO9991484.1"
                     /translation="FMASMGTQKLKAQGFFIFSLLLTLILFCITTLYNENTNVKLIPQ
                     MNYLMVVVALFFLNAVIFLFMLMKYFTNKQILPTLILSLAFLSGLIYLVETIVIIHKP
                     INGSTLIQTKSNDVSIFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIP
                     FLVFPLLAHNLSSYNADYSLYVVDYCPDNHTATWGINYTKILVCLWAFLLFFIIMRTR
                     LASELWPLIALLCLASLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQ
                     ELQLSSKLAVHDVLTNIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGH
                     RVGDKVLVSIVDIIQQSIRPDDILARLEGEVFGLLFTELNSAQAKIIAERMRKNVELL
                     TGFSNRYDVPEQMTISIGTVFSTGDTRNISLVMTEADQALREAKSEGGNKVIIHHI"
     gene            1529..3019
                     /gene="dgcJ"
                     /locus_tag="ACHKIR_003807"
     CDS             1529..3019
                     /gene="dgcJ"
                     /locus_tag="ACHKIR_003807"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0043709 - cell adhesion involved in
                     single-species biofilm formation [Evidence IEA];
                     GO_process: GO:1902201 - negative regulation of
                     bacterial-type flagellum-dependent cell motility [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcJ"
                     /protein_id="HFO9991485.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     RADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPEQRRAICEAFESANNTHGLNLTAHK
                     YPDLRGTLQTASTDCDTIVEAAALLPAFDQAVESNRHQDDYGSGLGMAEEKFHYYLDL
                     NDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQN
                     VMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIII
                     NQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHF
                     RLYQNVSRENISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDT
                     LGHQEGDLAITLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRL
                     QHIAPQKEIGFSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            3062..3565
                     /gene="yeaK"
                     /locus_tag="ACHKIR_003808"
     CDS             3062..3565
                     /gene="yeaK"
                     /locus_tag="ACHKIR_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HFO9991486.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGADFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTADYLRIAQPEL
                     VNFRRTA"
     gene            complement(3566..3670)
                     /gene="yoaI"
                     /locus_tag="ACHKIR_003809"
     CDS             complement(3566..3670)
                     /gene="yoaI"
                     /locus_tag="ACHKIR_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YoaI"
                     /protein_id="HFO9991487.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            3840..4286
                     /gene="yeaL"
                     /locus_tag="ACHKIR_003810"
     CDS             3840..4286
                     /gene="yeaL"
                     /locus_tag="ACHKIR_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFO9991488.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(4243..5064)
                     /gene="nimR"
                     /locus_tag="ACHKIR_003811"
     CDS             complement(4243..5064)
                     /gene="nimR"
                     /locus_tag="ACHKIR_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HFO9991489.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RAMVEMMAKEPVEWGSLGQWARFFAMSERNLARLIVKETGVSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            5161..6342
                     /gene="nimT"
                     /locus_tag="ACHKIR_003812"
     CDS             5161..6342
                     /gene="nimT"
                     /locus_tag="ACHKIR_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HFO9991490.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFTGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSTMVVPLA
                     LNGFGWQGALLMLMSFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLVYYVIIGWLPAILISHGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVAAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGDWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            6397..6744
                     /gene="yeaO"
                     /locus_tag="ACHKIR_003813"
     CDS             6397..6744
                     /gene="yeaO"
                     /locus_tag="ACHKIR_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFO9991491.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLANIAKKQPLTLLYSAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(6766..7020)
                     /gene="yoaF"
                     /locus_tag="ACHKIR_003814"
     CDS             complement(6766..7020)
                     /gene="yoaF"
                     /locus_tag="ACHKIR_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HFO9991492.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            7203..8228
                     /gene="dgcP"
                     /locus_tag="ACHKIR_003815"
     CDS             7203..8228
                     /gene="dgcP"
                     /locus_tag="ACHKIR_003815"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HFO9991493.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHPALHS"
     gene            8261..8359
                     /gene="yoaK"
                     /locus_tag="ACHKIR_003816"
     CDS             8261..8359
                     /gene="yoaK"
                     /locus_tag="ACHKIR_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HFO9991494.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            8362..8436
                     /gene="yoaJ"
                     /locus_tag="ACHKIR_003817"
     CDS             8362..8436
                     /gene="yoaJ"
                     /locus_tag="ACHKIR_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HFO9991495.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(8495..8743)
                     /gene="yeaQ"
                     /locus_tag="ACHKIR_003818"
     CDS             complement(8495..8743)
                     /gene="yeaQ"
                     /locus_tag="ACHKIR_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFO9991496.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(8891..9073)
                     /gene="yoaG"
                     /locus_tag="ACHKIR_003819"
     CDS             complement(8891..9073)
                     /gene="yoaG"
                     /locus_tag="ACHKIR_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HFO9991497.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYDTENEHDVCGW"
     gene            complement(9077..9436)
                     /gene="yeaR"
                     /locus_tag="ACHKIR_003820"
     CDS             complement(9077..9436)
                     /gene="yeaR"
                     /locus_tag="ACHKIR_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein YeaR"
                     /protein_id="HFO9991498.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSTEPDQVILIEAGQFAVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            complement(9609..10247)
                     /gene="leuE"
                     /locus_tag="ACHKIR_003821"
     CDS             complement(9609..10247)
                     /gene="leuE"
                     /locus_tag="ACHKIR_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HFO9991499.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NNEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(10374..11297)
                     /gene="dmlR"
                     /locus_tag="ACHKIR_003822"
     CDS             complement(10374..11297)
                     /gene="dmlR"
                     /locus_tag="ACHKIR_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HFO9991500.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITVEFLRQYFAEHYPNFSLEHA"
     gene            11400..12485
                     /gene="dmlA"
                     /locus_tag="ACHKIR_003823"
     CDS             11400..12485
                     /gene="dmlA"
                     /locus_tag="ACHKIR_003823"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HFO9991501.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFALSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKKIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVID
                     HGPKTPDMKGNATTPQVADAICKIILR"
     gene            12736..14346
                     /gene="yeaV"
                     /locus_tag="ACHKIR_003824"
     CDS             12736..14346
                     /gene="yeaV"
                     /locus_tag="ACHKIR_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HFO9991502.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANAIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGVAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISAWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYTPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHHV
                     EHYLPQTPVEVLEKTPVLPAGTVFKGDN"
     gene            14378..15502
                     /gene="yeaW"
                     /locus_tag="ACHKIR_003825"
     CDS             14378..15502
                     /gene="yeaW"
                     /locus_tag="ACHKIR_003825"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase, oxygenase subunit YeaW"
                     /protein_id="HFO9991503.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            15558..16523
                     /gene="yeaX"
                     /locus_tag="ACHKIR_003826"
     CDS             15558..16523
                     /gene="yeaX"
                     /locus_tag="ACHKIR_003826"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase reductase subunit YeaX"
                     /protein_id="HFO9991504.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHII
                     VQMSDGDNQYSNAYSLLSSPHNTSSYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHLAELQYSDVDWQLHYCTRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALNEAVRSEAARLNIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(16570..17685)
                     /gene="rnd"
                     /locus_tag="ACHKIR_003827"
     CDS             complement(16570..17685)
                     /gene="rnd"
                     /locus_tag="ACHKIR_003827"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HFO9991505.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGISDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQALPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(17767..19452)
                     /gene="fadD"
                     /locus_tag="ACHKIR_003828"
     CDS             complement(17767..19452)
                     /gene="fadD"
                     /locus_tag="ACHKIR_003828"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HFO9991506.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDSEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
BASE COUNT         4880 a         4892 c         4702 g         5159 t
ORIGIN      
        1 ttttatggct tctatgggta cacaaaaatt aaaagctcaa ggctttttta ttttcagctt
       61 attgctgacg ttaattttat tttgcattac taccttatat aacgaaaaca cgaatgtaaa
      121 actcatccca cagatgaatt acctgatggt tgttgtggct ttgtttttcc ttaacgccgt
      181 cattttcctt ttcatgttaa tgaagtattt cactaacaaa caaattttac caacactcat
      241 tttaagcctt gcatttttaa gtggccttat ctatttagtt gaaaccattg taattatcca
      301 taaaccaatc aacggcagta cactgatcca gacaaagtcg aatgatgttt ctattttcta
      361 tattttccgc caactcagtt tcatttgttt aacctcgctg gcgctctttt gttatgggaa
      421 agacaacatc cttgacaaca ataagaagaa aactggcatt ctgttgctgg cgctgattcc
      481 ttttttagtt tttccccttc tggcacacaa tctgagcagt tataacgctg actattcttt
      541 gtatgtcgtc gattactgtc cggacaacca tactgcgacc tggggaatca actatacaaa
      601 aatattggtt tgtttgtggg catttttact gttctttatt atcatgcgta cacgattagc
      661 cagcgaacta tggccgttaa tagcattatt atgtctggca tcgctatgct gcaacttact
      721 tctactgact ctggatgagt ataattacac catctggtat atcagtcgcg ggattgaagt
      781 ttccagtaaa ctgtttgttg tgtcttttct gatttataac atttttcagg aactgcaact
      841 ctccagcaaa ctggcagttc atgatgtact gaccaatatt tataatcggc gctacttttt
      901 caacagcgta gagtcattat tgtcgcgacc tgttgttaag gacttttgtg tcatgctggt
      961 tgatattaat cagttcaaac gcatcaatgc ccaatgggga catcgtgtgg gtgataaagt
     1021 gctggtatca attgtcgata ttatccagca aagcatccgc cccgatgata ttttagcgcg
     1081 actggagggt gaggtgtttg gcttgctatt taccgaactc aatagtgccc aggcaaaaat
     1141 cattgcggaa cgtatgcgta aaaatgtcga actcctgacc ggctttagta acagatatga
     1201 tgttcctgag caaatgacca tcagtattgg cacggttttt tcaacgggtg acacacgtaa
     1261 tatctcgctt gtcatgacgg aagcagatca agccttacgc gaagcgaaaa gcgagggggg
     1321 caacaaagtg attattcatc atatttaagt gcaaaaatat tcagagccat gctttttgcg
     1381 tggcttttgc atacaattta ttactattac ccctaaattt cccctcccca gcggcgcaga
     1441 gatgagtata attagcgccc ctgtgccagg ccgcaatcga actttatctg gttttctcgt
     1501 ttcactaacc gaaggagtgc catttatcat gaaattgcac catagaatgc tccggcattt
     1561 tatcgccgca agtgtcattg tgctgacatc ttccttcctt atttttgaac ttgtcgccag
     1621 cgacagagca atgagtgcct atctgcgcta tatcgtgcag agagcagact cctcctttct
     1681 ttatgataag tatcagaatc agagtattgc cgcgcatgtg atgcgcgctc tcgctgctga
     1741 gcagtcggaa gtgtcgccag aacagcggcg cgccatctgc gaggcttttg agtctgccaa
     1801 taatacccat ggcttaaacc tgactgccca taaatacccg gacttacgcg gcacactaca
     1861 aaccgcatcc actgactgcg acacaattgt ggaagctgca gcactattac ccgcttttga
     1921 tcaggcagtg gaaagcaacc gccaccagga tgattacggt tcaggtcttg ggatggccga
     1981 agagaaattt cactattatc tcgatctcaa tgaccgctat gtctattttt atgaaccggt
     2041 taatgttgag tactttgcga tgaataactg gtctttcctg cagtcaggaa gtattggcat
     2101 cgatcgcaaa gatattgaaa aggtatttac cgggcgtacc gttttatcga gcatttacca
     2161 ggatcagcgt actaaacaga acgtgatgag tttgctgacg ccggtatatg tcgcagggca
     2221 gctaaaaggg attgtgctgc tggatattaa caaaaacaat ctgcggaata tcttttacac
     2281 tcatgaccgc cctctcctct ggcgttttct caatgtcacg ctaaccgata ccgattcggg
     2341 gcgcgacatt atcatcaacc agagcgaaga taatctgttc cagtatgtca gttacgtcca
     2401 tgacttaccg ggcggcattc gtgtctcgtt atccattgat attctttact ttatcacgtc
     2461 ttcgtggaaa agcgttctgt tctggatttt gacggcgtta attttgctga atatggtgcg
     2521 gatgcacttc cgtttatacc aaaacgtgtc gcgagaaaat attagtgatg cgatgactgg
     2581 actgtataat cgcaaaattt taacccctga actggagcag cggttgcaga agctggtgca
     2641 atccggttct tcggtgatgt ttattgcaat tgacatggac aagttaaagc aaataaatga
     2701 caccctcggt catcaggagg gggatttagc gattacgtta ttagctcagg cgattaaaca
     2761 atcgattcgt aaaagtgatt atgccatccg actcggtggc gatgaattct gcatcattct
     2821 tgtcgattcg acgccgcaaa ttgcagcaca actgcctgaa cgtatcgaaa aacgtctgca
     2881 acatatcgcg ccgcagaaag agatcggctt ctcttccggt atttacgcga tgaaagaaaa
     2941 cgatacgtta catgatgcgt ataaagcttc cgatgagcgt ttatatgtca ataagcagaa
     3001 caaaaacagc cgttcatgat aaccttctgt ggttgtttgc ttgtaatctc aggagcatga
     3061 aatgactgaa atggctaaag gaagcgtgac gcatcagcgg ttaatcgctt tattatcaca
     3121 agaaggtgct gacttccgcg tcgttaccca tgaagcggta ggtaaatgtg aagcggtgtc
     3181 agaaattcgt ggcaccgctt taggccaggg ggctaaggca ctggtatgta aagtcaaagg
     3241 caatggtgta aatcaacatg ttctggcgat cctcgcggcc gatcaacagg ccgatctgag
     3301 ccaactcgcc agccatatag gcggattacg cgcttcgttg gccagcccgg cggaagtcga
     3361 tgaactcaca ggctgtgtct tcggcgcgat cccccctttc agcttccatc caaaactcaa
     3421 actggttgcc gatcccctac tcttcgaacg atttgatgaa atcgccttca atgcaggcat
     3481 gctggataaa tccgttattt tgaaaaccgc ggattatttg cgcattgcgc aaccagaact
     3541 ggtcaatttc cgccgcactg cgtaactagc ctgtccgttc gataagaaga acggacaaaa
     3601 ccagcacaac agcaatggca aaaaacgatg acgtgataat cagtgtctcg acgaacattt
     3661 gatcgttcat agtatgcccc cgggagatag ttgttttcac gttattgtta gcgtgcgcaa
     3721 atggcagttt gatgacagtt cgcctgattt tttatgcaaa aaacgtaaag attttctact
     3781 tccttcctgc agcaagcgta aagtaagcag gcttattatt ctttggcaag gaaaccacga
     3841 tgtttgatgt cactttgctg atcctgctcg ggttagctgc gctgggcttt atcagtcata
     3901 acaccactgt cgccgtttca attctggtgt taatcattgt ccgcgtcaca ccgttaagca
     3961 cctttttccc gtggattgaa aaacaggggc taagtatcgg gataatcatt ctgacgattg
     4021 gcgttatggc tcccatcgcc agcgggacgc taccgccctc aaccctgatt cactcgtttc
     4081 tgaactggaa atcgctggtg gcaattgcag tcggcgttat tgtctcctgg ctgggggggc
     4141 gcggcgtgac gttaatgggc agccagccgc aactggtcgc cgggctgctg gtgggcactg
     4201 ttttgggcgt ggcgctgttt cgcggcgtac cggttggccc gcttattgcc gcgggtctgg
     4261 tttcgctgat tgtggggaaa cagtagttaa tcccgcaata tatcggcctg gcgtttgccc
     4321 caggcctttc ttaaacatag taataaaggc cgttgttgaa tcatacccca gcgtatgagc
     4381 aactttctgt acagtatcgc ctttcactaa tccctgaagc gccataatca gctgtaactg
     4441 ctgacgccac tggcgaaaac tcacccccgt ctctttcact atcagtcggg caagattgcg
     4501 ttcactcatc gcaaaaaagc gagcccattg ccccaatgac ccccactcga cgggctcttt
     4561 cgccatcatc tccaccatcg cgcggatttt aggatgagaa gaaacgggta ggtgcagctg
     4621 ttgttgtggc tgctgaggga gttcatcaaa caggacctga attaatctgc gggtcatcgg
     4681 ctcagcacgc tgagtcgtcg tcctgtttgc cagggttaaa attaattccc ggcataaggg
     4741 cgatattttc aatgtgcaac aggttgtcgg cattgtcact gcgctgggtt cgataaacag
     4801 aaaacagagt tcagcgttag ctgtcacttg attgctatgt tccacccctc ccggtatcca
     4861 aaccgcatat tgtggcggca ccatccacaa agcattttcc accgtacagg taattgcacc
     4921 atgtagtgcc agtatcaact gtcctttgcg gtgctgatgt acaggactgg ttaactcatc
     4981 cgttccggcg tgaatacaaa atgcgaccgc ggcttcatgg tgtcggtcgg gttcatagcc
     5041 attgagattc aacctgtgca tcattttgtc cgaatttagc gataatttgt cattttagct
     5101 tgattcaaca taacaataaa aacggtaagg tacagcctcg tttgtaacaa tgagaagcat
     5161 atgacctgtt caacttcatt aagcggcaaa aacaggattg tccttatcgc tggcattctg
     5221 atgattgcca caacattacg cgtcaccttt accggcgcag caccgttact ggatacgatt
     5281 cgttccgctt actcgctgac gacggcgcaa accggcttat tgaccaccct gccattatta
     5341 gcctttgcgc taatctcacc tttggctgcc ccggtggcgc gacgttttgg tatggaacgt
     5401 agcctgtttg ccgcgttact tttgatctgt gctggtatcg caattcgctc tctcccttcg
     5461 ccttacttat tatttggcgg tacagcggtc attggcggtg ggattgcatt aggcaatgtc
     5521 ttactgccag gattaattaa acgcgatttc cctcattccg tcgccagact taccggcgca
     5581 tattccctga caatgggagc tgcagcggca ctgggatcga ctatggtcgt gccgctggct
     5641 ttgaacggtt ttggctggca aggcgcgttg ctcatgctga tgtcttttcc tctgctggct
     5701 ctttttttat ggctgccaca gtggcgaagt caacaacatg caaatttgag tacctcacgc
     5761 gccttacata ctcggggtat ctggcgttcg ccgcttgcct ggcaggtaac attgtttctt
     5821 gggatcaact cactggtcta ttacgtgatt attggctggc ttccggcgat tctcatcagt
     5881 cacggctata gcgaagcaca ggcgggttca ctgcatggtt tgctgcaact agccacagca
     5941 gctcccggtt tgctgatccc acttttctta catcatgtga aagatcagcg tggtattgca
     6001 gcgttcgttg ccttgatgtg cgcagtggcc gcggttgggc tctgctttat gccagcacac
     6061 gcgatcacct ggactctgct tttcggtttt ggttccggcg caacaatgat actggggttg
     6121 acgttcattg gtctgcgggc aagttctgcg catcaggcgg cggcactctc ggggatggca
     6181 caatccgtcg ggtatttgct ggcagcctgt gggccgccgc tgatgggtaa aatacacgat
     6241 gctaacggtg actggtccgt accactgatg ggtgttgcaa tactttcact actgatggcg
     6301 attttcggac tttgcgccgg gagagacaaa gaaattcgct aatatccggt gctatagtga
     6361 cgtggcaaat catgcgtgaa agggagaaca aatacgatga atattcagtg caaacgcgtt
     6421 tatgatccgg ctgaacagag cgatggttat cgcgtactgg tcgaccgcct ctggccacgc
     6481 ggtatcaaaa aaaccgattt agcccttgat gaatgggata aagaaatcac gccgtcaacg
     6541 gaactgcgca aagcctttca cggcgaagtc gtcgattttg caacctttcg cgagcaatat
     6601 cttgcagaac tggcgcaaca cgagcaagaa ggaaagcggc tggcgaacat cgccaaaaaa
     6661 cagccgctga ccctgctcta ctcagcaaaa aacaccacgc agaaccatgc gctggtgctg
     6721 gcagactggc tacgtagctt gtgattttag tacgccatcc ggcagttatt tttcaccagc
     6781 cggatggtcc cgtcgccaca atgcccattc atcaatcgtt tcaccgcccg gtaatttgca
     6841 attgttgctg accccttgcg ctgtctgcac aggaatgagc gtcccgccct tctgctggca
     6901 atagaccgac gccggatttg ccataccaat ctgcggcggt ttaggtgctt ctggctgaga
     6961 aggggttgaa caaccagcca ggaccagcaa gcaaggcaga acaaaactga taattttcat
     7021 ttattgatct cacatattta tccagactta gagtatcgcg gtatcgtttt gttttgcagc
     7081 actattttta ttacattcac tcaaatcata ttacgccttg tttcatcttt gttgatgatg
     7141 ttttatcatg cctgcaaaga ttaaataatc agcatttacc cgccgtatcc tggagttgtt
     7201 tcgtgtcaga tcagattatc gcccgcgtct cgcaatccct tgccaaagaa cagtcactgg
     7261 aaagcctggt ccgacagctt ctggagatgc tggaaatggt cactgatatg gaatcaacct
     7321 acctgaccaa agtggatgtc gaagcgcgcc tgcagcatat aatgtttgcc cgtaacagcc
     7381 agaaaatgca catcccggag aattttaccg tctcgtggga ttactcgtta tgcaaacgcg
     7441 ccattgatga aaactgcttt ttcagcgatg aagtccctga ccgttggggc gactgtattg
     7501 cggcacgcaa tcttggcatc accacatttc tgagcacgcc aattcactta ccggatggat
     7561 cattctatgg cacgctttgc gccgccagca gtgagaagcg ccagtggagt gaacgcgcgg
     7621 aacaggtttt acagttattc gccggactga ttgcacaata tattcaaaaa gaggcactgg
     7681 ttgagcagct gcgcgaagcc aatgctgcgc tgattgcgca atcgtatacc gactcgttaa
     7741 ccgggctacc gaatcggcgg gcgatttttg aaaatctgac gacgctgttt tctctcgccc
     7801 gacatcttaa ccataagata atgatcgcgt ttatcgatct ggataacttc aaattaatca
     7861 acgatcgttt tggtcataat agtggtgatt tgttcctcat tcaggttggc gagcgcctta
     7921 atacgctcca gcaaaatggc gaagttattg gtcgtctcgg cggtgatgag tttttggttg
     7981 tttcactgaa caacgagaat gcggatattt cgtctctgcg agaacgtatt caacagcaaa
     8041 tacgtggaga atatcactta ggtgacgttg atttgtatta tcccggtgcc agtcttggca
     8101 tagtagaagt cgatcctgaa acgaccgatg cagacagtgc cctgcatgct gccgatatcg
     8161 cgatgtatca ggagaaaaaa cacaaacaga aaacaccttt tgtcgcgcat ccagcgctac
     8221 attcctgagg cgtattcaca tccttttgat tggtgataac atgcgaatcg gtattatttt
     8281 tccggttgta atctttatta cagcggtcgt atttttagca tggtttttta ttggcggcta
     8341 tgctgccccg ggagcataaa gatgaaaaaa acaacgatta ttatgatggg tgtggcgatt
     8401 attgtcgtac tcggcactga gctgggatgg tggtaacgtc acatctagaa aatagcaaag
     8461 gctgcctgtg tgcagccttt gtgcaattta cgcgttaact tttaatcttt ctgtagataa
     8521 acagcacgac aatcgcgcca ataacggcaa ccacgaagct gccaaaattg aagccatcga
     8581 ctttaccaaa gccaaacagc gtgctgatcc atccgccgac tacggcaccg actatcccca
     8641 gcaggatagt cataaagaat ccaccaccat ctttacctgg catgatccac ttcgccagaa
     8701 taccggcaat aagcccaaaa ataatccatg acagaatgcc cattgtttcc tcacttatct
     8761 gttttgcatt agcgggttag tcgctgataa aaagcatagc acaacatcgt gagggcaaga
     8821 tttgtgacga gcatcacgga ggattttttt gcgatggcgc agaaattgcg ccatcaacga
     8881 tcagtgataa ttaccaacca caaacatcat gttcgttttc cgtgtcataa gaacgcacgg
     8941 tattcaccag atcttttatc acttcagccg ccacttctgg caccagcaaa gtcatcggcg
     9001 tctctgtttc ataatcgaca gaaacgccat tgctgttatt ggtgacggtc acggtatacg
     9061 ttgctttgcc catgattcat ttcccgttat gaatgacttt ccgttgttgc gcaccttcca
     9121 tcaggacttc aggagcaacg aagaagtcaa tgttgaaata agtgtcgtca gtcatggctt
     9181 caatgttgtg ccacttttct ggagggaaca cagcaaactg ccccgcttcg ataaggatca
     9241 cttgatcagg ctctgtactg tgttcatcag cgtagccgag atatttgacc gccccatgca
     9301 taacggaaag gcgtgggtaa acccccgggc gcgttccttt atcaagatga cgttcgaata
     9361 ttccggcagg cgcagtttgt ttattccaga aaggcgttga gcgcgtatga atataattct
     9421 gtgggatttg cagcatcctt ttccctcctt cggtgaatgc gctgaaaacg gtttatttca
     9481 gccgtttcag ggtacgcctg ataatttgca ttttaaatac catttattgg ttactattta
     9541 gcaccgtatc agagaagaat cagggaggat tacaggtggg aagcccgcga caacgcgggc
     9601 tgaatgcatc aggactggag cgtcgccagt cgggcggcga aacccacaaa catcaaacca
     9661 atcagtgagt tgcccacttt agccagtttc tttttggtac gtatgtactg cgtgacaaaa
     9721 gcaccagaaa taatcaggaa gctcaaatag cagaaactca ccagttccag cgtcgtcgcc
     9781 agaataaaga atgaaattcc cgtatgtggg gcattaacat cgataaactg tacgaaaaac
     9841 gacacataga acaaaatggc tttcggatta gtcaggctca aaattaacgc gcgtttaaaa
     9901 atggcaccgt attggggctc atcggatttg gcctcgttat ttttaccctt cagggtcgcg
     9961 tagagaattt tactccccag atagagcaaa taaaacgcac caagataacg tacaatgttg
    10021 aataatatcg gggtggtctt aattaatgtc gccactccag cccatgccag aaacatcaat
    10081 accgcatcgc caataaatac accgcaggcc gcaagataac cgcctttcat accgctactg
    10141 acgctatttt tgagtacaaa cagggtattt ggccctggca ccaacacaat aaaaatggcc
    10201 ccaaccagat aggtccagta attcagaacc ccgtattcag cgaacacatt aacctcttta
    10261 attatctttc gatcatgcgc gattaaaggt gaatatgcta accaatctgt agcggcttag
    10321 aaaggagaaa atcaggtttt aacctgatat caacccgata attgaatcat taatcaggca
    10381 tgctccagtg aaaaattcgg gtagtgctcg gcaaaatact ggcgtaaaaa ttctaccgtt
    10441 atccgtactt tcgctgacgt cgccagcctt gaaacataaa cggcccagac attcgctggc
    10501 tggtaatatt ccggtagcac ttgtactaaa tgaccactgg caatgttttc gctaacatcc
    10561 caccaggagc gcagagcaat cccctgccca tcgagacacc actgatggac aatttctcca
    10621 tgattagacg acagtgggcc ggtaacttta atcgcatggt ggccttcttt attgcgtaat
    10681 tgccagacac caaaaggatg atcgcgctct ttaatgacca ggcacggcaa tgcagaaaga
    10741 tcggttaaat gtttcggcgc gccatgttgc gcaataaatt ccggtgacgc gcagagaatg
    10801 cgataattgg tcgccagttt gcgggcgatt aaattagggg cgatgtcatc gccaatgcga
    10861 atatcgagat cgacaccttc attgaccaaa tcgaccaacc ggtcttccac atcaaaacgt
    10921 aattccagtt gcggatacgc cttcgccagc gctgataacg ccggggccac cacctgtcgt
    10981 ccaaaaccaa agctgctgat aatacgcagc atcccctgtg gcacctgacg cacgtcagaa
    11041 agttcgtcca tcatctgacc gacatcctgc aaaatccgct gcgcccattc ataaatccgc
    11101 tctccttctt cggtaatggt gacgcggcgg gtggtgcggt gtaacaacac cacgtttagc
    11161 gtttgctcca gcaaggcgat gcgcttgctg acgaacgccg gagaaacgcc cagttcttcc
    11221 gccacggcgg caaaaccagc ccggcgagcc accagcataa agacgcgcaa atcgttcagc
    11281 agcggtaaat tattcatgat tcgtgtttta tgtttcacca gttacaggga ttaattcctt
    11341 ttcagtcaat tataggatgg tgatgttgtc aattttgatg gtcaggaagt gagaacccaa
    11401 tgatgaaaac gatgcgtatt gctgcgatcc cgggagacgg gattggcaaa gaagtccttc
    11461 ctgaagggat tcgcgtgtta caggctgccg ctgagcgctg gggcttcgcc ttgagttttg
    11521 agcaaatgga gtgggcgagc tgcgagtatt acagccatca cggtaaaatg atgccggacg
    11581 actggcatga gcaacttagc cgtttcgacg ccatctattt tggtgccgtc ggctggccgg
    11641 acaccgttcc ggaccatatt tcgttgtggg gttcgctgct gaaatttcgt cgtgaattcg
    11701 accagtacgt caacctgcgc ccggttcgtc tctttcctgg cgttccctgc ccgctggcgg
    11761 gaaaacagcc tggcgacatc gatttttacg tggtcaggga aaacaccgaa ggcgaatatt
    11821 cctcgctcgg cggcagagtg aatgaaggta cagagcatga agtcgtcatt caggaatcgg
    11881 tatttactcg tcgtggtgtc gatcgtattt tgcgctatgc ctttgaactt gcgcaaagcc
    11941 gcccacgtaa gacgctaact tctgccacta aatcgaacgg tttagccatc agcatgccgt
    12001 actgggatga gcgagtggaa gcaatggctg agaattaccc ggagatccgc tgggacaagc
    12061 agcatattga cattctctgc gcacgttttg tgatgcaacc ggaacgcttc gatgtggtag
    12121 tggcgtccaa tttgtttggc gatatccttt ccgatcttgg cccggcctgc accggcacca
    12181 ttggcattgc cccatccgcc aacctgaatc cggaacgcac tttcccgtcg ctcttcgagc
    12241 ctgtccacgg ttccgcgccg gatatctacg ggaaaaaaat tgctaaccct atcgccacaa
    12301 tttgggccgg tgcaatgatg ctcgattttc tcggcaatgg agatgagcgt ttccagcaag
    12361 cgcataacgg tattctggcc gcgattgaag aagtgattga tcacgggccg aaaacacctg
    12421 atatgaaagg caatgccacc acgccacagg ttgccgacgc gatttgcaaa attattttgc
    12481 gttaaggtca aaccagttta tttgaaccgc gtcactgacg cggttttttt attcgttctt
    12541 tgcagtaaat aacctgcgtc atttcacctt ttattgtttc cgtttcgtgt tttatggctt
    12601 tccgtattct taattgttta atttatgtaa catgcaaata atcaatttgt taatcacgac
    12661 atacaactca ttaccctgca aataattaca actggcaaaa gccagagacg ggcttttatg
    12721 cccaggagat gactcatgat gagcaatgtt aagaaaaaag atgtgccgct gataagcatc
    12781 agcctggtgg ccattctttt catcgcagct gcattaagcc ttttcccaca acaatcggcc
    12841 gacgcggcca acgcaatata cacttttgtt acgcgtacgt taggttccgc cgtacaggta
    12901 ttggttttgc tggcaatggg actggtgatt tatttagcca ccagtaaata cggcaatatt
    12961 cgtcttggcg aaggaaaacc ggaatacagc acgctctcat ggctgtttat gtttatttgt
    13021 gccggtttag gttcttctac gctttattgg ggggttgctg aatgggccta ttattatcaa
    13081 acgcctggat taaatatcgc accgcgttca caacaggcac tcgaatttag cgttccctac
    13141 tccttcttcc actggggcat cagcgcctgg gcaacttata cgctggcctc attaatcatg
    13201 gcttatcact ttcatgtgcg gaaaaataaa gggctgagcc tttccggcat tattgctgct
    13261 attaccggcg ttcgcccgca aggcccgtgg ggaaaactgg tcgatttgat gttcctgatc
    13321 gccactgtcg gcgcactgac catttccctt gttgtcaccg cagcaacctt tacccgtggg
    13381 ctttccgcgc tgaccggttt acccgataac tttaccgtac aggcatttgt gatcctgctt
    13441 tccggcggca ttttttgcct aagctcgtgg attggtatca acaacggttt gcaacgtcta
    13501 agcaaaatgg ttggctgggg cgcgttcctg ctgccattac tggtgctgat tgtcggtcca
    13561 accgaattta ttaccaacag catcatcaat gccatcggcc tgaccacgca aaacttcctg
    13621 caaatgagct tattcaccga tccgcttggc gatggttcat ttacccgcaa ctggaccgtt
    13681 ttctactggc tgtggtggat ctcatacacg cctggcgtag caatgtttgt cacccgcgtt
    13741 tcccgcggtc gtaagattaa agaagttatc tggggactga tcctcggcag caccgtcggt
    13801 tgctggttct tcttcggcgt aatggaaagc tatgccattc accagtttat caatggcgta
    13861 atcaacgtcc cacaggtgct ggaaacactg ggcggcgaga cagctgtgca gcaagttctg
    13921 atgtcgttgc cagccggtaa attgttcctc gccgcatacc tgggcgtgat gattattttc
    13981 cttgcctcgc atatggatgc ggtggcctac accatggcgg caaccagtac gcgtaatctc
    14041 caggaaggtg acgatcctga ccgtgggctg cgtcttttct ggtgcgtggt aattaccctg
    14101 atcccgcttt caattctttt cactggcgcg tcgctggaaa cgatgaaaac caccgtcgta
    14161 ctcacagccc ttccgttcct cgtcatttta ctggtgaaag tcggcggatt tattcgctgg
    14221 ctgaaacagg attacgccga cattccggct catcatgttg aacattatct cccgcagaca
    14281 ccggttgaag tcctggaaaa aacgccagtg ctccctgcgg gaaccgtatt caaaggcgac
    14341 aactgagcgc catcatccta acgataaagg tatccctatg agcaatctga gccctgactt
    14401 tgtactaccc gaaaattttt gcgctaaccc gcaagaggcg tggaccattc ctgcccgttt
    14461 ttataccgat cagaacgcct ttgaacacga aaaagagaac gtcttcgcca aaagctggat
    14521 ttgcgtcgct cacagcagcg aactggcgaa tgccaatgat tatgtgacgc gtgagatcat
    14581 tggcgaaagc attgtgctgg tacgcggtcg tgataaggtt ttgcgcgcgt tctataacgt
    14641 gtgtccgcac cgtggtcatc agttgttgag cggcgaaggg aaagcgaaaa atgtgattac
    14701 ctgcccgtat cacgcatggg cattcaaact cgatggcaac ctggcccatg cacgtaactg
    14761 cgaaaacgtc gccaatttcg atagcgacaa agcgcaactg gttccggtgc gtctggaaga
    14821 atatgccgga ttcgtcttca tcaacatgga ccctaacgcc accagcgtag aagatcagtt
    14881 acccggtctg ggcgcgaaag tgctggaagc ctgcccggaa gtccacgatc tgaaactggc
    14941 ggcccgcttt accacccgca cgcctgccaa ctggaagaac attgtcgata actatctcga
    15001 gtgctatcac tgtggtccgg cgcatccagg tttctccgac tccgtacagg ttgatcgtta
    15061 ctggcacacc atgcacggta actggacgct gcaatacggt ttcgccaaac cgtcagaaca
    15121 gtctttcaaa ttcgaagagg gcacggatgc ggcattccac ggtttctggc tgtggccgtg
    15181 cacgatgctt aacgtcacac cgatcaaagg gatgatgacg gtcatttatg aattcccggt
    15241 ggattctgaa actaccctgc aaaactacga tatttacttc accaatgaag agttaaccga
    15301 cgagcaaaaa tcgctgattg agtggtatcg cgatgtgttc cgtccggaag atttacgtct
    15361 ggttgaaagc gtacagaaag ggctgaaatc gcgtggctat cgtggtcagg ggcgcatcat
    15421 ggccgacagt agcggtagcg gaatttccga acatggtatc gcccatttcc ataatctgct
    15481 ggcgcaggtg tttaaggact aatgacatcg gcggcggtat tttccgccgc tgggctgatt
    15541 tttgatggag tacagcaatg tcagactatc aaatgtttga agtacaggtg agtcaggtgg
    15601 aaccgcttac cgaacaggtg aaacgcttca cgctggtggc aaccgatggc aaaccgctgc
    15661 ctgcgttcac cggaggaagt cacatcatag tgcagatgag cgatggtgat aaccagtaca
    15721 gcaatgcgta ttcactactg agttcaccgc ataacacctc tagttatcag attgccgttc
    15781 gactggagga aaactcgcgc ggcggttccc gctttttgca tcagcaggta aaagtgggcg
    15841 atcggttaac gatttcaacg cctaataacc tgtttgcgct aattccctcg gccagaaagc
    15901 atctgtttat cgcgggcggt attggtatca cccctttcct gtcgcacctg gcagagttgc
    15961 aatacagcga cgtcgactgg cagctacatt actgcacgcg aaatccagaa agttgcgcat
    16021 ttcgtgatga gctagtccag catccacagg ctgagaaagt tcacttacat cactcatcaa
    16081 ccggaacacg actggaatta gcgcgattat tggcggatat cgaacctggc acacatgttt
    16141 atacctgtgg ccccgaggcg ctaaatgaag cggtaagaag tgaagctgcg cgtctgaaca
    16201 tcgccgccga tacgctgcac tttgagcaat ttgctatcga agacaaaacc ggcgatgcat
    16261 ttaccctggt gcttgcccgt tccggaaaag agtttgtggt gccggaagag atgactattt
    16321 tgcaggttat tgaaaataat aaagcagcga aagtggaatg tttatgtcgt gaaggggtat
    16381 gcggaacctg cgaaacggca atactggaag gtgaagctga ccatcgggat cagtatttta
    16441 gcgatgaaga gcgtgccagc cagcaaagta tgttgatctg ttgttcgcgc gcgaagggta
    16501 aacgcctggt gttggatttg tagtttgccg aagccggact gagcgcgccc agtccggctt
    16561 cggaagattt tactgcggat attcctgcaa taaattgtgt aatgcttccg ccatcagctc
    16621 accacgccag ccggaaatca gctccggcaa attgttctgc ggtttcagtt tccagtgcca
    16681 gttcagcagt tggttgattt gccgacgcga tgccagcaat tcggcgctga tcttgtgcgt
    16741 ttcgctcacg tcagtaatca gcgacttaat cgctttaaac gctttacgat aacccggcat
    16801 gtccatcagg ttaagcatcg gctgcggtaa ggcctcttcc ggcaatgcct gcgctttttc
    16861 caccagcgcc agcagcgttt taccgtgaaa gcggatttcg ctaccggata agcccaggct
    16921 gtccagttcg cctaaacttc ccggcatata acgcgctacg gaccacaaat gctcttcacg
    16981 cacgacaaag ttcactgcca gatcgcgctc ccgcgccttg cgcagtcgcc agtcggctaa
    17041 cagttgcaga caggccagtt ggcgtgtgcg taactgccag gcattggtga tatcacgcca
    17101 ggcatcttcc ggagcaacga cttcctgacg acgcatttgc atcaggcggc attcatccag
    17161 cgccgcaggt agccagccgg aggcctccgt ttctaccata agctttgcgg tgatcggtaa
    17221 cagataccag acatccgccg ctgcgtattc acactgacgt tcggtcagcg gtctggccag
    17281 ccagtcggtg cgcgattcac tcttgtccag cgtaacgccg gaatactctt ccaccatgga
    17341 agcgaaaccc catgacatcg ggcgtccgca gaaggcagca aggatttgcg tgtcaatcaa
    17401 gggttgtggt aattcgccaa agacattgag gaacacttcc agatcttcac tgcccgcatg
    17461 gagaaatttt gtgatggacg gatcgcgcag gatcgctttc agcggtgacc agtcagagat
    17521 cccgagggga tcaattagcg ccagatgctc accatcaaaa agttgaatta gacccagttg
    17581 cgggtaatag gtgcgcgtac gaacaaattc agtatccagg gctatcgccg gaaaggcacg
    17641 gacggcttca cacaaagaag ccagcgcatc gtccgtggta atcatttggt aattcaaatt
    17701 gtattctctt tagtgggcgt caaaaaaaac gccggattaa ccggcgtctg acgactgact
    17761 taacgctcag gctttattgt ccactttgcc gcgcgcttcg tcacgtaatt ctcgtcgcaa
    17821 aatttttccg acgttagatt tcggtaactc atcacgaaac tccaccagct tcggtacttt
    17881 gtagcccgtg agctgacggc ggcaaaaagt caccagtgac tcttcggtaa gcgatggatc
    17941 ttttttcaca acgaagattt tcaccgcttc accactggag ccggaaggta cgccaacagc
    18001 cgcgacttcc tgtacgccag gatgctgcat gacgacatct tcaatctcgt tgggatagac
    18061 gttaaaaccg gaaaccagaa tcatgtcttt tttacgatcg acaatgcgca ggaatccttc
    18121 ttcatccatc accgcgatgt cgccggtgtg taaccagcca tttttgatga tttcatcggt
    18181 agcatccgga cgctgccagt aacccagcat cacctgcggt cctttgacac aaagctcacc
    18241 cggttgacca ggtggtactt cactatcatc atcatccacc agtttggctt ccgtcgacgg
    18301 caccggcaaa ccgatgctac cactatgata atcaatatca tacgggttaa cgctcaccag
    18361 cggcgcacac tcggtcaggc cataaccttc cagcagatac tgtccagtca gtttaaccca
    18421 acgttctgcc accacttgct gaacaggcat ccctccgcct gcggaaagat gcagactgga
    18481 gaaatccagc tgctggaact ctttattgtt cagcaacgca ttgaacaagg tgttaacgcc
    18541 agtgatagcg gtaaacggat atttcgctaa ctcttttacc aaccctggaa tatcgcgcgg
    18601 gttagtgata agcaggttct gcccccccag ttcgataaac agcaggcagt taatggtcag
    18661 tgcaaaaatg tgatacagcg gcagcgccgt caccaccagc tctttgcccg gatgcaacag
    18721 cggaccatag gtcgcgttaa cctgttccag gttcgccagc atattgcggt gagtcagcat
    18781 cgcgcctttc gccacaccag tggtgccgcc ggtgtattgc aggaaagcta aatcttccgg
    18841 caccagttcg ggtttgacgt actgcatccg gtagccgttg tgcagggcgc tacgaaatga
    18901 aatggcatct ggcagatggt atttcggcac cagacgcttg atgtatttaa caacgaaatt
    18961 gactaccgtg ccttttgccg tagatagctg atcgcccata cgggtcagaa ttacgtgctg
    19021 aacggcggtt ttatcaacca ctttttccag agtgtgggca aagttagaca cgataacaat
    19081 cgccgatgcg ccgctatcgt taagctgatg ctcaagttca cgcggggtat acaacgggtt
    19141 aacgtttacg acaatcatcc cggcacgcaa aatgccaaac agcgccaccg gatattgcaa
    19201 taaattaggc atcatcaacg caacgcgatc gcctttcttc agtcccaacc cttgttgcaa
    19261 ataagcggca aacgcgcgac tgcgttcttc cagtttgcgg aaggtcatca cctcccccat
    19321 attcacaaac gcgggttgat cggcgtagcg cgcgaccgac tgctcaaaca tatctaccag
    19381 agattgataa cggtcagggt tgatctccgt cgggacgtcc gcgggataac ggttaagcca
    19441 aaccttcttc aattcttcac ctctaaaatg cgtgttcgtc gtcatcgcaa ccccaaatga
    19501 tatacatgct gttaacataa tattaactca tcataccagc ttgataatta cccaacgaaa
    19561 aggttgcgaa gcgcgtcact atttattttt atctttaccg taagaatgca gaaacagcgg
    19621 accagccgct gtt
//
LOCUS       DAZGQL010000090        19458 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.090, whole genome shotgun sequence.
ACCESSION   DAZGQL010000090 DAZGQL010000000
VERSION     DAZGQL010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19458
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.090"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(72..818)
                     /gene="cutC"
                     /locus_tag="ACHKIR_003829"
     CDS             complement(72..818)
                     /gene="cutC"
                     /locus_tag="ACHKIR_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFO9991507.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLNTLNNLAELGVARVLTSGQKSDALQGLSK
                     IMELIAHGDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DAHADEYSRYVVDGAAVAEMKGIIERHQAK"
     gene            complement(832..1398)
                     /gene="yecM"
                     /locus_tag="ACHKIR_003830"
     CDS             complement(832..1398)
                     /gene="yecM"
                     /locus_tag="ACHKIR_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFO9991508.1"
                     /translation="MANWHSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNATAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            1614..3347
                     /gene="argS"
                     /locus_tag="ACHKIR_003831"
     CDS             1614..3347
                     /gene="argS"
                     /locus_tag="ACHKIR_003831"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFO9991509.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEIDEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            complement(3400..3792)
                     /gene="flhe"
                     /locus_tag="ACHKIR_003832"
     CDS             complement(3400..3792)
                     /gene="flhe"
                     /locus_tag="ACHKIR_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HFO9991510.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGVTLNHRGESMSSAPLS
                     TRQPAAGLMTLVAWRYQLIGPTPAGLRVRLCSQSRCVELEGQSGTTVAFSGIPAIEPL
                     RFIWEVSGGGRLIPPLKVQRNEVIVNYR"
     gene            complement(3792..5870)
                     /gene="flhA"
                     /locus_tag="ACHKIR_003833"
     CDS             complement(3792..5870)
                     /gene="flhA"
                     /locus_tag="ACHKIR_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HFO9991511.1"
                     /translation="MSNLAAMLRLPANLKSTHWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGREQKA
                     PAEPKPVKMAENNTVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMDLQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            complement(5863..7011)
                     /gene="flhB"
                     /locus_tag="ACHKIR_003834"
     CDS             complement(5863..7011)
                     /gene="flhB"
                     /locus_tag="ACHKIR_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HFO9991512.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVCV
                     IWFGGVSLARRLSGMLSAGLHFDHSIINDPNLILGQIILLIREAMLALLPLISGVVLV
                     ALISPVMLGGLVFSGKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKSILVGSVTG
                     FYLWHHWPQMMRLMAESPITAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKK
                     LRMSRQDIRDEFKQSEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVAL
                     QYDENKMSAPKVVAKGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQ
                     LYAAVAEVLAWVWQLKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
     gene            complement(7200..7844)
                     /gene="cheZ"
                     /locus_tag="ACHKIR_003835"
     CDS             complement(7200..7844)
                     /gene="cheZ"
                     /locus_tag="ACHKIR_003835"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HFO9991513.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQGIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(7855..8244)
                     /gene="cheY"
                     /locus_tag="ACHKIR_003836"
     CDS             complement(7855..8244)
                     /gene="cheY"
                     /locus_tag="ACHKIR_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HFO9991514.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(8259..9308)
                     /gene="cheB"
                     /locus_tag="ACHKIR_003837"
     CDS             complement(8259..9308)
                     /gene="cheB"
                     /locus_tag="ACHKIR_003837"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HFO9991515.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAVGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            complement(9311..10171)
                     /gene="cheR"
                     /locus_tag="ACHKIR_003838"
     CDS             complement(9311..10171)
                     /gene="cheR"
                     /locus_tag="ACHKIR_003838"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HFO9991516.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNLHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTVPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQTTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(10190..11791)
                     /gene="tap"
                     /locus_tag="ACHKIR_003839"
     CDS             complement(10190..11791)
                     /gene="tap"
                     /locus_tag="ACHKIR_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HFO9991517.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQESQNAFD
                     VNFEAWQLEINHVLESASAQSQRNYHISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSQEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMTDIVSSVTRVND
                     IMGEIASASEEQRRGIEQVAQAVSQMDQVTQQNASLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(11837..13498)
                     /gene="tar"
                     /locus_tag="ACHKIR_003840"
     CDS             complement(11837..13498)
                     /gene="tar"
                     /locus_tag="ACHKIR_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HFO9991518.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYHTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVSRVDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRIAEQDPNWETF"
     gene            complement(13643..14146)
                     /gene="cheW"
                     /locus_tag="ACHKIR_003841"
     CDS             complement(13643..14146)
                     /gene="cheW"
                     /locus_tag="ACHKIR_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HFO9991519.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(14167..16131)
                     /gene="cheA"
                     /locus_tag="ACHKIR_003842"
     CDS             complement(14167..16131)
                     /gene="cheA"
                     /locus_tag="ACHKIR_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HFO9991520.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRDQSPR
                     RIILSRLKAGEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKISPPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(16136..17062)
                     /gene="motB"
                     /locus_tag="ACHKIR_003843"
     CDS             complement(16136..17062)
                     /gene="motB"
                     /locus_tag="ACHKIR_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HFO9991521.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLNGGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(17059..17946)
                     /gene="motA"
                     /locus_tag="ACHKIR_003844"
     CDS             complement(17059..17946)
                     /gene="motA"
                     /locus_tag="ACHKIR_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HFO9991522.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLEFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGVVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(18073..18651)
                     /gene="flhC"
                     /locus_tag="ACHKIR_003845"
     CDS             complement(18073..18651)
                     /gene="flhC"
                     /locus_tag="ACHKIR_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HFO9991523.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(18654..19004)
                     /gene="flhD"
                     /locus_tag="ACHKIR_003846"
     CDS             complement(18654..19004)
                     /gene="flhD"
                     /locus_tag="ACHKIR_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HFO9991524.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
BASE COUNT         4610 a         5441 c         4784 g         4623 t
ORIGIN      
        1 gcccctgttg aaattgcagg ggcctggtac gagcaagcat catattgggc gacatgatgc
       61 aacggtaaaa atcatttggc ctgatggcgt tcaatgattc ctttcatttc agcaaccgcc
      121 gccccgtcta cgacataacg cgaatactcg tccgcgtgtg catctgatga catggacaat
      181 ccttgattac gataacgcat tggtgaggct tgccacgctc ccgcggagct atggacttcc
      241 agcactccgg catcgaggaa gtggtgcagg ttttctgcac ggactcctgc tccggccata
      301 atgattggag catcaccatg ggcaataagt tccataattt ttgataaacc ttgcagtgcg
      361 tctgattttt gccctgatgt cagtacccga gcaacgccca attccgcaag attattgagt
      421 gtatttaaag ggttagcgca catatcgaag gcgcgatgaa aagtcacggc cagcggaccg
      481 gcagcagcca ttattttttc cattcgtggc atatcgacat tcccgtcaac atcgagaaca
      541 cccgtcacca gtccaggaaa acctaattcg cggactgtgc gcacatcctc aagaatggcg
      601 gcaaactcac cgtcgctgta gcaaaaatca ccaccgcgtg ggcgaataat aggatgcaca
      661 gggatcgtca cccgctggcg cacggatttc agcaccccca acgacggcgt taagcccccc
      721 tcttttgggg ctgcgcataa ttcaactctg tctgcgccgt tttgctgcgc cgttagcgcg
      781 cattccatgc tgtaacagca aatttccagt aatgccattt ttgctcctta attacgccga
      841 ctgctcgctg gcaacgatct cttcaatgga ccacggatga aatttaatgg tcgttttgcc
      901 atcggtgacc gccagcgtcg gattcggtaa tcgctcatgc tcaccttttg gcgaactggt
      961 tttaactgag atccccggca ggctgagtcc ttcatcagaa agcaatgcca gcgcacgggc
     1021 attcagcgtt tctggatcgc ccggcagaac gatttcaata tgttcccaac cttcgtgcgg
     1081 gtaacgtttt tccccgggcc acggtagctc cacaatggaa aactgccagt gcgcaacctg
     1141 taccggttca tgcaatttaa acagacaaat cggcctgcca ttgatcatat tttctgacaa
     1201 aagctcgccg cactgttcaa acccgcgacg ccagcgctca gcggtggcgt tttgatggca
     1261 acgcaaagaa atgtgatcgg cagtcagcgg agtgatatcc aaccccaaac ggcgggaaag
     1321 ttcatctaat gcgtgggtaa atcgcggtaa atccgatgca atatcctgca gctcgtcgat
     1381 agaatgccag ttcgccataa tcactcttcg tcttccacta aaagcgttaa tttaccctgt
     1441 tgccctgcgc caaccaaccg ctgatttcac gccgcttctg atgcaatagt gaaaacgaca
     1501 atacgccacg cgcacgttgc tgacgaaaac agccatttgc agtatactcc cgccctaatt
     1561 tctttaactg gtgcgggtaa tttttgctcg cttcatcaat gtaaggtatt ccggtgaata
     1621 ttcaggctct tctctcagaa aaagtccgtc aggccatgat tgcggcaggc gcgcctgcgg
     1681 attgcgaacc gcaggttcgt cagtcagcaa aagttcagtt cggcgactat caagctaacg
     1741 gcatgatggc agttgctaaa aaactgggta tggcaccgcg acaattagca gagcaggtgc
     1801 tgactcatct ggatcttaac ggtatcgcca gcaaagttga gatcgcgggt cctggcttta
     1861 tcaacatttt ccttgatccg gcattcctgg ctgaacatgt tcagcaggcg ctggcgtccg
     1921 atcgtctcgg tgttgctacg ccagaaaaac agaccattgt ggttgactac tctgcgccaa
     1981 acgtggcgaa agagatgcac gttggtcacc tgcgctctac cattattggt gacgcggcag
     2041 tgcgtactct ggagttcctc ggtcacaaag tgattcgcgc aaaccacgtc ggcgactggg
     2101 gcactcagtt cggtatgttg attgcgtggc tggaaaagca gcagcaggaa aacgccggtg
     2161 aaatggagct ggctgacctt gaaggtttct accgcgatgc gaaaaagcat tacgacgaag
     2221 atgaagagtt tgccgagcgt gcacgtaact acgtggtaaa actgcaaagc ggtgacgaat
     2281 atttccgcga gatgtggcgc aaactggtcg acatcaccat gacgcagaac cagatcacct
     2341 acgatcgtct caacgtgacg ctgacccgtg atgacgtgat gggtgaaagc ctctacaacc
     2401 caatgctgcc gggtatcgtg gcggacctga aagccaaagg tctggcagta gaaagtgaag
     2461 gcgcgaccgt cgtattcctt gatgagttca aaaacaagga aggcgaaccg atgggcgtga
     2521 tcattcagaa gaaagatggt ggctatctct acaccaccac tgatatcgcc tgtgcgaaat
     2581 atcgttatga aacactgcat gccgatcgcg tgctgtatta catcgactcc cgtcagcatc
     2641 aacacctgat gcaggcgtgg gctatcgtcc gtaaagcagg ctatgtaccg gaatccgtac
     2701 cgctggaaca ccacatgttc ggcatgatgc tgggcaaaga cggcaaaccg ttcaaaaccc
     2761 gcgcgggtgg tacagtgaaa ctggccgatc tgctggatga agccctggaa cgtgcacgcc
     2821 gtctggtggc agaaaagaac ccggatatgc cagccgacga gctggaaaaa ctggctaacg
     2881 cggttggtat tggtgcggtg aaatatgcgg atctctccaa aaaccgcacc acggactaca
     2941 tcttcgactg ggacaacatg ctggcgtttg agggtaatac cgcgccatac atgcagtatg
     3001 catacacgcg tgtattgtcc gtgttccgta aagcagaaat tgacgaagag caactggctg
     3061 cagctccggt tatcatccgt gaagatcgtg aagcgcaact ggcagctcgc ctgctgcagt
     3121 ttgaagaaac cctcaccgtg gttgcccgtg aaggcacgcc gcatgtaatg tgtgcttacc
     3181 tgtacgatct ggccggtctg ttctctggtt tctacgagca ctgcccgatc ctcagcgcag
     3241 aaaacgaaga agtgcgtaac agccgtctga aactggcaca actgacggcg aagacgctga
     3301 aactgggtct ggatacgctg ggtattgaga cagtagagcg tatgtaatcg cgttatacgg
     3361 caaaagccga tgtcatggca gacatcggct tttttatcat taacgataat tcacaatcac
     3421 ttcattccgt tgtaccttta gcggcggaat taaccgccca ccgccagaca cttcccagat
     3481 aaatcgcaac ggttctatcg ccggtatacc ggaaaaggcc acggtggttc cactctgccc
     3541 ttctaattcg acacaacgag attgtgaaca caagcgaacc cgcagtcctg caggtgtcgg
     3601 gccgataagc tgataacgcc acgccaccag cgtcatcaat cctgcagcag gttgtcgagt
     3661 agaaagaggc gctgacgaca tcgactcacc acggtgattc agcgtaacgc ccacactact
     3721 cgcctgccac atcccctccc cggcggcttg caccagcagc ggaaacaata atattgctaa
     3781 taaagttctc atcatttacc gccaattgtc gccgtcatgc ggatatgtcg gttatcagat
     3841 agttccagat tcgacagcac cactaactgc ggcaggctgc ggcgcaggaa gcgagacaat
     3901 aatggtcgca gcgcgtggtt caccaacaat accggcggcg cacccaacat ctcctgacgg
     3961 gatagcgctt cctgagtttg cgccagtaaa cgatccgcca gccctggctc cagtcccccc
     4021 ccgccctgca acgcctgtag caacaaacgt tccagcggtg tatcgaggcc aataacatgg
     4081 acttcatctt tgccaggaaa ccactgctgg gtaatcgccc ggcccaacgc tacgcgcacg
     4141 acggcggtta attcatgcgg atcgctttgg atgggcgcat gttccgccag cgtttcgaga
     4201 atggtgcgca tatcgcgaat cggcactttt tcatcgagca gattttgcag cactttatgc
     4261 agtgtggtaa gcgtgacgac accaggaacg agatcttccg tcagctttgg catctcctgg
     4321 gcgacgcgat ccagcagctg ttgtgcctcc tgacgaccaa acagctctgc ggcatgctgg
     4381 ctaatgaggt ggttaagatg ggttgccacc accgtgctgg cctcaaccac ggtgtacccc
     4441 tgaatctgcg cctgttcttt aagcgcactt tcaatccaga tagcattcag gccaaatgcc
     4501 ggatcgacgg tcgcctcacc aggtaacgtc ccggcagcgg taccagggtt aatcgccaac
     4561 caacgccccg gataagcatc accactgcct atctccacgc ctttcatcaa aatgcgatag
     4621 cgggcaggtt gcagatccat attgtcgcga atgtgcacca ccggcggcag aaaccccatc
     4681 tcctgggcaa atttcttgcg aatactgcgt atacggccca gcaactcacc atcctgctgg
     4741 aaatcgacca tcgggatcag gcgataaccc acttccattc ccagagaatc ttccagttgc
     4801 acatcgttcc acgtcgcttc gacaacggta ttattctctg ccatttttac cggttttggt
     4861 tcggcaggcg ctttttgttc acgtccgcgg atccaccagg ccagtcccag caatccggca
     4921 gtgaacagca aaaataccag gttcggcatt ccaggcacca ggccgagtaa accgagcacg
     4981 gcggcgctta acaacataac gcttgggtta ctgaaaagct gattcaccat ctgctcgcca
     5041 acatcctgat cggtgctgac acgcgtaacg atgacccccg cggcggtaga aatcaccaac
     5101 gccggaattt gtgccaccag accgtcgcca atagtcaata gcgtataact ttccgccgcg
     5161 tgtcccatgc tcataccatg ttgcagcacg ccgaccagca acccaccgac aacgtttatg
     5221 accatgatga ggatcccggc gatggcatcg ccgcgaacaa acttacttgc cccgtccatc
     5281 gagccgtaaa aatcggcttc ctgagtcact tcggagcggc gttttttcgc ttcatcttca
     5341 ccaatcaatc cggcgttaag gtcggcgtca atcgccatct gcttccccgg cataccatcg
     5401 agaacaaagc gcgcgcctac ttccgcaata cgcccggcac ctttggtaat gaccataaag
     5461 ttgatgatca cgagaatgac gaacaccacg ataccgatag cgaaattgcc accaacgagg
     5521 aagtgaccga acgcttcgac cacctttcca gccgccgccg cgccggtatg cccttccatc
     5581 aaaatgatac gggttgaagc cacattaagt gccagacgca acaacgtggt aaacaacaga
     5641 atcgtcggaa acgcagcaaa ctcaagcgtg cgttgggtaa acatcgccac cagcaacacc
     5701 atgatcgaca aggcaatatt gaaggtaaac agcaggtcga gtatgaatgc gggcagtggc
     5761 agtaccatca tcgacaagat caacaggatc aaaatcggtc cggcaaggat ctgccagtgt
     5821 gtcgatttca ggtttgcggg caggcgcagc atcgcggcca gattactcat gggtcggttt
     5881 ctcgttaata aaatccaggg cttccggcac cggaagatga gtaggttgta cagggcgctg
     5941 tccaccagcc agacgccagc gtttcagttg ccagacccag gccagcactt ccgccaccgc
     6001 ggcgtacagt tgacccggga tttgttgacc gatctccgca tgtcgataca gcgcacgcgc
     6061 cagcggcggc gcttcaagcg tcgggacgtt attttcagcg ccaatttcac gaatgcgcag
     6121 cgcgaccagc cctgcacctt tagcgaccac tttcggtgcg ctcattttgt tttcgtcata
     6181 ctgcaaagcc actgagtagt gagttgggtt attgacgata acgtccgctt tcggcacatc
     6241 ggccatcatc cgccgccgcg ccgcagctcg ctgcatctga cggattcgcc ctttgacatg
     6301 agggtcgcct tcgctttgtt tgaactcatc acgaatatcc tgccgtgaca tacgcagctt
     6361 tttcaggtgg ctgaagattt ggaaaaagac gtcaaatccc accatcggaa tgacaccaag
     6421 caccaccagc aatgcgcaca gccctaccag atccatcgca ttacccatgg cggtaatggg
     6481 ggactccgcc attaagcgca tcatttgcgg ccagtggtgc cagagataaa accccgtcac
     6541 actgccgacc aggatggatt tcaaaatcgc tttaagcaac tccgcaccgg tttgtgccga
     6601 aaacatccgt ttaatgcccg gtagcgggtt gagtttggaa aacttcggct gcaaggattt
     6661 gccgctaaat accaggccac ccaacataac cggagaaata agcgccacca gcaccacacc
     6721 gctaatcagc ggcagcagcg ccagcatggc ttctctgatc agcagaataa tttgcccgag
     6781 gatcagattc gggtcattga taatactgtg atcaaaatgc agcccagcgg agagcatgcc
     6841 cgacaatcga cgggccagcg acacaccgcc aaaccagata acacacacgc ccactaacaa
     6901 aatcagcagt gacgttaatt cacgtgaacg cgggatttgc ccctcttccc gcgctttttc
     6961 tagtcggtga ggtgtggggg cttctgtttt gtcgtcgctc tcgtcagaca cgtcgccaat
     7021 cctggaaaga gtattaagcg taaatgatgc cagagcgcaa agcgttcaat ggtttgagta
     7081 aggggcaaaa caggcgggat ttagggcttt tactgccaca catcaagcat agtatgcgtt
     7141 tgtcggatgc ggcgtcatcg cctgatatga cgtggtcacg ccacatcagg caatacaaat
     7201 caaaatccaa gactatccaa caaatcgtcc acctgatcct gactggctac cacaccggct
     7261 ttgctggtat cgacctgagg tccattaagc aaactctggt tttcacgttt tggacgcgac
     7321 tcctgttccg ggatgttttc caacagcacc atcaacaact ggcgttcgat cccctgaatg
     7381 acatccatca tccgcttaat aacctgcccg gtgagatcct gaaaatcctg cgccatcatg
     7441 atttccagca gttgcgcgtt agtaaagctg gtatgcgcgg ggacatctgc cagaaattgc
     7501 cgcgtatctg ttaccagttc tcgggcgtcg gcaaggtcaa tcggatcggc aaaccagtca
     7561 tcccaacgtt gggttaacgc ttttgctgat ttctccattt gatcctgatg cggttgtgac
     7621 gcctcaacac tgttcagcgc ccgctccgca gcctgggcag tcatctgcac aacatagtac
     7681 aaacgatcgc gcgcatcggg gatggcttcc gccgcttcgg caatggcctg atccagcccc
     7741 agttcccgca aactgtcgcg cagcatacgc gtcaggctgc cgatgcgcgc aatgatatcg
     7801 ccagctgaat gctcgtcagc aggtttgatt gatggttgca tcatagtcgc atcctcacat
     7861 gcccagtttc tcaaagattt tgttgagttt ttcctccagc gtcgcggcgg taaatggctt
     7921 caccacatag ccactggccc ccgcttgcgc cgcagcaata atgttctctt tcttcgcttc
     7981 tgcagtcacc attaacactg gcaatgctga catcgcgcca tccgcacgaa tcgttttcag
     8041 caattccagg ccatccatgt tgggcatgtt ccagtcggag ataacaaatc cataaccgcc
     8101 tgcctgcaac ttattgagag cgtcgacacc atcttccgct tcctcaacat tattgaatcc
     8161 tagctctttc agcaggttac gcactatgcg tcgcatggtg gaaaagtcat ccacaaccaa
     8221 aaatttaagt tctttatccg ccatttcaca ctcctgattt aaatacgtat cgcctgtccg
     8281 gcactaattt ttgccagcat ttgctggctt acctggctaa gatcgaccac ttcgcaaaca
     8341 ccacccatat tgatggcctc gcgcggcatg ccgaacacca cgcaacttgc ttcgttttgt
     8401 gcaagggtcc atgcccccgc ctgacgcatc gccaacattc ccaccgcgcc gtcgttgccc
     8461 ataccggtca ggatcacccc aaccgcatta cgccccgcct gtttggcgac agaatggaac
     8521 aacacatcta ccgaaggccg atgacggtta accgccgggc catcgtgaat tttgatttgg
     8581 taatttgcgc cactacgcgc cagctccata tgccgatcgc ccggcgcaat ataagcatgc
     8641 cccggcaaga cacgttctcc gtcttcggct tctttaaccc cgatctggca aagcttatta
     8701 agtctgtcgg caaaagagcg ggtgaaaccg ggcggcatat gctgggtaat taacagtgcc
     8761 gggctggaaa gcggcaacgg ttgcagtacg tgacgaattg cctcagttcc acccgttgaa
     8821 gcaccaatcg caatcagttt ttcagagctc aacaacggcc ctgccttcag cgttgtcggt
     8881 gccgacaatg gcttatgtgc tgcaaggctc gcctttgctg ctgtacgcac cttttcagca
     8941 atcatttcgc tatacgccag cattccttcg cgaataccca gttgcggttt ggtgacaaaa
     9001 tctatcgccc ccagctccaa tgcgcgcagc gtgacttctg atcctttgcc ggtcagggaa
     9061 gaaaccatca caacgggcat tgggcgcaaa cgcattaatt tttcgagaaa atccagtccg
     9121 tccatccgcg gcatttcaac atccagcgtt agcacatccg gattgaattt cttaatcaaa
     9181 tcacgcgcaa ccagcggatc aggggcggtc gccaccattt ccatgtcgct atggctgttg
     9241 atgatttctg tcatgatctg gcgcatcagt gccgaatcat cgacagataa caccctgatt
     9301 ttgctcatcg ttaatcctta cttagcgcat acaccgtctg accacgcagc gtgaagcggc
     9361 gctcaaggtg gctaaagttt tcagagtgac ccgcaaacag caatccgtcg ggtttaagga
     9421 gcggaacaaa gcggcgcaaa atctcctgct gggtagtttg atcgaagtag atcatgacgt
     9481 tacgacagaa gatcgcatca aacggccccg gcacggtgta ctgtttcgcc agtaaattca
     9541 gcggggcaaa atcaacatag ttcgccagct cctgacgcac gcgtaccagc ccttcatgcg
     9601 gtcccgtccc tcgcatgaaa taccgttgca gttgctgcgg cgtcaggttt ttcaactctt
     9661 catggcgata aataccgctt ctggcttttt ccagcacttc ggtgtcgata tcgctggcaa
     9721 acactttcca gcgtccgggc acggtgccca atgtgtcagc cagcgtcatc gcaatgctgt
     9781 acggctcttc gccggtcgaa gccgccgcgc tccatacgcg atactcgcca gaacggcgac
     9841 gtgcgtgatc cgcgagcaga ggaaaatgat gcgcctcacg gaaaaatgcc gtcagattcg
     9901 tggtcagcga attgataaac gcctgccact caccgctgtg cagattagat tccagcaagt
     9961 tcaggtaatg accgaaatcc gtcagtccca gcgaacgcaa acgacgaacc agtcggttgt
    10021 aaaccatgtc gcgtttatgg tcggccagga cgatcccggc tcgttgatag atcaattgac
    10081 ttatccgccg aaaatgcgcg tcggaaagcg ccaggcgctc ggtcatctgt aacaataaag
    10141 acgtttgccc acagggcaga gatgaagtca tagcgccttc tcaatcactt caggatacca
    10201 ctggcgcaat ttgtaactgc gccgactcat gtcgtgctac ttcatgctct tcaagggtaa
    10261 ataccgccac gcgcgacgaa agatggtcgg cctgattcgc cagttgttct gttgccaccg
    10321 ccgcttcttc taccagggag gcgttctgct gtgtcacctg atccatctgg ctgacagcct
    10381 gtgcaacctg ctcaatcccc cgccgttgtt cttccgaggc cgaagcaatt tctcccataa
    10441 tgtcgttcac gcgggtcacc gaactgacaa tatcagtcat ggtcgcggcg gcgttattca
    10501 ccagtttcga accctgctgg acacgattga ctgactcttc gatcagccct ttgatctctt
    10561 ttgctgcctg ggcgctacgg ctggcaagat tgcgcacttc acctgccact accgcaaaac
    10621 cacgcccctg ctctccggcg cgagccgctt ccactgccgc attcagggcc agaatattgg
    10681 tctggaaagc aatgccgtcg ataacgctga taatgtcgcc aattttttgc gagctggtag
    10741 cgatctcctg catggtgtga gtcatggtac tgacctgaac accgcccgcc tgcgccgttg
    10801 tcgcggcatt ttttgccagt tccgacgcct gtcgtgcgtt atcggcgttt tgccctaccg
    10861 tggcggttaa ttgctccata ctggcggccg tttgtgccag cgatgccgcc tgctgttcgg
    10921 tgcgacttga gagatcgtta ttgcctgcga caatctccgc gataccaatg tgcatctcct
    10981 ggcttccctt acgcacatca cttaccgtcc cacgcaaagc ctgctgcatg gtcttcaggc
    11041 tggcaaaaat ggcggtgatc tcattgcgac catacaccgc aatcggacgt gccagattac
    11101 ccgcagcaat gctgtcaaaa tggctaccga taatggccag tggttgaaca atcatcttgc
    11161 gcgtccacca cagcgcactg ctgatgtaga tcgccgcaac aataatcatg ctgataaaca
    11221 ccagcgccga aatgtgatag ttacgctggc tttgcgcact ggcactttcc agcacatggt
    11281 tgatctccag ctgccaggcc tcaaagttaa cgtcaaacgc attctgtgat tcctgcaccg
    11341 gcgcagtgag gaaatccgaa agttgattac tttcaagcca ggtcgcctga tgttcgagat
    11401 cgttgtgcca gcgggcaaaa cttttctccg tctctttttg caacgccctg acgtgctcgt
    11461 tgcccgcagt catcgccata aaacttttaa acagcgtggt ggattgcgtc agactggcgc
    11521 gcgccgtcgt catcaacgtt ttaatgtcat ccgccggata gctaagcgcc gtcagagtgc
    11581 ccgctttgtt cagcgcggta ctggcctgta acattaccgc ccgagtttgc gctaatgccg
    11641 cacgttgctg attgctctgc tcgacctgat tcagtcgttg caaatcgtcg cgaaatgccc
    11701 aaaaagacat gccgttactg ccaatctgca agatcccgca gagaatcaaa attaaaaaca
    11761 gcgtggtcga aattcgaata cgattaaaca tcaacgctcc ccatcaggcg gcaatgaccg
    11821 cgttagtaaa tactcgtcaa aatgtttccc agtttggatc ttgttcagca attcgcagtc
    11881 gtggctgtgc cggtggttgc tcactggcag gacgggatgg tgtttgcggt ttattggtga
    11941 gtgggctggc tgccagacgg aacgcggaaa ccgcttgcgt taaacgactc gcctgttctt
    12001 ccagcgcagc tgcggcggca gctgattcct gcaccagcga tgcgttctgt tgcgtgacgc
    12061 gatccatttc cgaaaccgcc aatgcgactt gatcgatgcc acggctctgt tcatccgatg
    12121 ccgatgcaat ctcgcccata atgtcagtca cgcgggtgac agcattgacg atattgttca
    12181 ttgtttcccc ggcgctttcg accagcaccg aaccggtatc aacgcgcgag acggagtctt
    12241 caatgagggc tttgatctct tttgccgcct gggcgctgcg actggcaaga ttacgcactt
    12301 cgcccgccac cacggcaaaa ccacggccct gttcacccgc tcgcgcggct tcaacagcgg
    12361 cattcagcgc gagtatatta gtctggaagg caataccatc gataacgctg ataatgtcgg
    12421 caattttctt cgaactatcg gcgatctcat gcatcgtttt cactacgcca tccaccactt
    12481 tgccgccgtg ctgggcggtg tcggaggcac tttgcgccag ttgcgaggcc tggcgggcgt
    12541 tatcggcgtt ttgcttcact gtcgcggtga gctgctccat gctggcggca gtttcttcca
    12601 gcgcggatgc ctgctgttcc gtacgggagg aaagatcggt gttgcccgcc gcaatttcac
    12661 gggtaccggc atagatggca tctgaacctt cacgcacatg agtgacggtg tcagtcaaag
    12721 agcgttgcat atgtgaaacg ctctgcgcca ggtcgcccat ttcactgcgc ccgtcaatgg
    12781 tcagggtatt cgccaggtta ccaccggcga tttcgcgaat gtgagcaata atttttgcca
    12841 gcggtgtaag caacatacgg cgaatgccgt accacgccac cagcagaatc aataccacca
    12901 ccagcgcgat aaccgccagt tgccactggg caaatcggta atcatctgcg ttgtcagtga
    12961 cgatatcgcg atacagtttt tcactgctga gggcgtactg agcaaacgct tcgcccattg
    13021 cattttgcat tccctgggtt ggctgagcga aataagctcc agtattgcca taatcaagat
    13081 aatcaatcag ttcagttaac gctgtgtgat agtttttata tttttcatca atattacgac
    13141 tggtagcgac catttcaggt aacggtgcca tgcttttgaa ttttttataa tgagtcgctg
    13201 cctgggccaa tgttttcctg gcgctatcga gcaattcaac tttggcatta ctttgctgat
    13261 tggatgaatc catcatcatc cgcaccgctg aacgactcag gttaatgcgc gtttgcagca
    13321 ttaaatccca tgttgacgtc agctcgccct gctgttcccg taattgattg gaaaccacaa
    13381 agctcttctg gctatggtga agggaagaaa aaaacagact gccggaaata agctgtaaca
    13441 gtgcgaatac ccccagcacc attaccaaca gcgtgactac gcggatacgg ttaatcataa
    13501 ggcaccttcc tgaaaacaag ttgatctcgt tatcggcaag gaagggggaa actttattgc
    13561 tgatgccacc cgccgcgaaa ctgaaataaa aaacccgatg cgcagatcat cgggttcatt
    13621 gcaattgagg aaatcgggag aattacgcca cttctgacgc cgcgctatct aatagcgcca
    13681 tctcttcgct gttcagcagt ttttcgatgt tcaccagaat caacatacgg tcgcccagtg
    13741 cacccagtcc agtgagatat tctgttgaaa gcgtcacggc aaattccggt gccggacgaa
    13801 tttgctccgc cgtcaatgaa agcacgtctg agacgccgtc aaccacgatg ccgaccaccc
    13861 gctgtccgag attcaggacg ataactaccg tgttgtcgtt atagtccaca tccacctggc
    13921 tgaacttaat tcgtaagtcc acaatcggca caataacacc gcgcagattc gtgacgcctt
    13981 tgataaacgc tggcgtgttc gcaatccgtg ttacctgatc gtagccacgg atctcctgca
    14041 ctttcagaat atcaataccg tactcttcat caccaagggt aaataccaga aattcctggc
    14101 ctgacggctc gctggccagc tttgttacat tcgtcatacc ggtcatattg ttaccttttt
    14161 actcattcag gcggcggtgt tcgccatacg ttgttcgcgg tttatcgcct gcaaggcgga
    14221 gacatcaaca atcagtgcca cgctgccgtc gccaagaatg gtcgcagcag aaatgccggg
    14281 gactttgcga tagttacttt caaggttttt aaccacaacc tggtgttgac caattaattg
    14341 atccaccagc aaggcgtagc ggcgaccgcc actttgtaag atcaccacaa ttccctgggt
    14401 agcttcggtt ttcgcgcccg cgacgttgaa cactttccac agttcgacga tgggcagata
    14461 ttcaccccgc acttccagca cccgctcgcc gccagccagt ggatgcagat cggcttcacg
    14521 gggttgcagt gattccatga cagcattcag cggcagaatg aaaacttcat ccgcaacgcg
    14581 tacggacatg ccgtcgagga tggccagcgt cagcggcagt aaaatgcgga tcgtcgtgcc
    14641 agtaccctgc ttcgactgga tttcgacatg accgcccatc tcctggatat tacgtttaac
    14701 gacgtccatg ccgacgccgc gcccggagac gtcggtgacc tgctccgccg tggagaagcc
    14761 aggggcaaat atcagcatcg cgacttcgtc gtcgctcatg ttttcgctga cggtcagacc
    14821 ttgcgaggcc gcttttgcca gaattcgctc acggtttagc cccgcaccat cgtcggtcac
    14881 ttcaatgcaa atgttgccgc cctgatgttc ggcagacaga attaaatttc cgacgctgtt
    14941 tttacctgcg gcgagccgtt tttctggcag ttcaataccg tgatcgaggc tattgcgtac
    15001 caggtgggtc agcgggtcga taatgcgttc tatcaggctc ttgtcgagtt cagtggaact
    15061 gcccaccagc gtcagttcta cctgcttgcc gagttttccc gcaagatcac gcaccagccg
    15121 gggatagcga ctaaagacat actccatcgg catcatgcga atcgacatca ctgattcctg
    15181 caaatcacgg gcgttacgtt gtaactgccc catgctggta atcaaatcac catgattaac
    15241 cgggtccagt tcactagaac gctgggcaag cattgactgg gtgatgacca gctcgccgac
    15301 gaggttaatt aattgatcaa ccttttctac cgctacacgg atgctggtgg attcactgct
    15361 gcgcgtcgtt ttttcccgct ccacgcggcc ggttggcgct tgttcggctg ccagtttaag
    15421 cactggtggg ggggatattt tgggcgagac ttctactgtt tcaaaggtaa tctgatcggc
    15481 ttcaatcaca aaacagagta ccgctgtgat gtcatcttcg gcgatgtcgc ccggtaatat
    15541 tgccgagaga gaatccgccc ctttcaccac atcagttaac gttgtcagat gccccagctc
    15601 ttcttccagc aggtcgactt ccccggcctt caggcgcgaa aggataattc gtcgcggcga
    15661 ctgatcgcgg ctctgctcat cttgcggttc acttttggca accacactta atcgggtcac
    15721 tgcagatggc gtttcgcctt tcgcttctaa tgccagttga cgcaaggcct ggcagatata
    15781 atcgaagctg gcggcatccg gctcttgcga ctgtttataa gcgtcgagct gttcttgcat
    15841 gatgtccttc gtttccaaaa acagattgat aatgtcggtg ttgagttgca tctcacctcg
    15901 tctggcttcg tcgagcaggt tttccatcag atgcgtggtt tcctgcaaaa cgctgaagcc
    15961 aaaggttcct gcccctcctt tgatcgagtg ggcagcccga aagatggcat tcaattgttc
    16021 ggcatctggc gcttccggct gcaaaaccag cagatgctgc tccatgtcag ccaacagttc
    16081 gtccgcttca tcaaaaaatg tctgataaaa atcgcttata tccatgctca cgctgtcacc
    16141 tcggttcggc tgatggcatt gtgggaacac tgacctgtgg tgcaacctca ggtttttcca
    16201 gggcgcttac tggctcattc tggctttcgg cgttttcatg caaaatggcc tgttcggctt
    16261 gtttgttcag taccagcagg ctgatgcgac ggttgacggc atcatcaggt ccgcgatcgc
    16321 ttaagcgcat cgttgccgcc atgccgacga cacgtaacac tttgccgcca ttcaacccac
    16381 caaccatcag ttcgcggcgg gatgcattgg cacgatcggc agaaagctcc cagttgctgt
    16441 aacctttctc accgctggcg tagggaaaat catcggtatg acccgagagg ctaattcggt
    16501 tgggaatacc gttcagtacg ggcgcgatgg cgcgcagaat gtcgcgcata tagggttcga
    16561 cgtcggcact gccagttcta aacatcgggc gattctggct atcgatgatc tgaatacgta
    16621 gaccttcctg gaccagatcg attttgagat gtggacgtaa cgcccgcagt ttcggatcgg
    16681 actcaatcaa ctggtcgaga tcgccacgca atttccgcaa tcgactttgc tccatgcgtt
    16741 ttttcagctc ttcgatgttc ggctgcttat tcacttcccc ctggctttgg gtgtaatcat
    16801 cacccccacc gggaattggg ctttcactat tagaaatgcg atcgccgccc gtaaccgcag
    16861 tcgccagtgg agtccggaag tactccgcaa tctgaatcag ctcttttggg ctggagatgg
    16921 agattaacca catcaccaga aaaaaggcca tcatcgcggt cataaagtcg gcataagcaa
    16981 tcttccacga tccatgtgct gccccgtggc ttttggcttt gcgtcgtttg acgacaataa
    17041 tcggatgcgc ttgattcttc atgcttcctc ggttgtcgtc tgttgttgcg gatttttcac
    17101 cgcacgcaca tgctcttcca gttcaataaa cgacggacgt tcgctggaat agagcgtttt
    17161 gcgaccaaac tcaacggcga taggcggtgc gtaaccgttc agattagata gcagtgtgac
    17221 tttgacgcac tgcatcattt tgctggtttc ggcgcttttc tgacgtaaaa cactcgctaa
    17281 tggggaaata aatccgtaag ccaataaaat accgaggaaa gtccccacca tcgcatgtgc
    17341 gataagcgcc cccagctcgg cggcaggacg atcggctgaa cctaacgcgt gaacgacccc
    17401 cattacagcc gcaacaatac caaacgccgg aagtgagtca ccgaccagcg ccagactgtt
    17461 cgccgggact tctgcctcgc tttcgtgcgt ctcaatctct tcatccatca gagcttcgat
    17521 ttcgaaggtg ttcatgtgac cgctgataat caggcgcaga taatcgacga taaattcaag
    17581 catgacgcta tccgcaagga tgcgtgggta gctggcgaag atctcgctct cacggggatt
    17641 ttcaatatca cgttccagcg aaaacatccc catctgccgc gatttcgcca tcaaccgata
    17701 aagcagagcc agcagatcca tatacattgc tttggtgtat ttggagcgac gaaacagcaa
    17761 cggcagcgcc ttcagcgtgc ctttaatcgc tttgccatta ttgccgacga taaacgaccc
    17821 aatccctgca ccggcaataa tcaccagttc agcgggttga tagagtgctc caaggcttcc
    17881 accggtcatc aaataaccgc cgaaaactgt accgagaaca accaggtaac ctaataagat
    17941 aagcacgaca tcatccttcc actgttgacc atgacaggat gttcagtcgt caggcgttaa
    18001 cacgcgatag gggcaaaaaa aagcagcggt acgtcgttac cgctgctgga atgtttcgcc
    18061 acaccgtatc agttaaacag cctgtactct ctgttcatcc agcagttgtg ggataatatc
    18121 ggcaggattc tgggaaagtt tacgtctttt tactgcccgg gatggcggct gacataagct
    18181 gcaggcaaag ctgccaacag gctggtgagc gtgggtaata aaattgccgc cgcagcagtt
    18241 gcagctggaa agttgcagta atccactttc aacaaaccgc accaatgtcc aggcacgggt
    18301 taatgccagc agcggtccct cttctgcttg cgggcactgt tcaaggtata aacggtaggc
    18361 tttgatcacc gcatcgacgc cattacacaa accggttttc agtaaaaact gccatgcgtt
    18421 acagaacatc gaagcatgaa cgttttgttc ccaggtcata aaccagtcgg ttgagaatgg
    18481 cagcatgcct ttcggcggtg ggcttccgcg cagttcttta tagagtttta tcaggcgtcc
    18541 gcgacttaac tgtgtttcgc tttccagcat ctgcaaacga gcgcccaggg tgatcaattc
    18601 cattgccagc tgaatatccc gcgcttcctg aacaatgctt ttttcactca tgatcaggcc
    18661 cttttcttgc gcagcgcttc ttcaggctga ttaacatcat tcagcaagcg tgttgagagc
    18721 atgatgccgg tatgaatttg ctggagatcg tcaacgcggg aatcttgcgt caactgagta
    18781 atcgtctggt gactgtcaaa acggaagtga caaaccagtt gattggtttc tgccagctta
    18841 accatttgcg gaagagtcag tgccgctaac gttgtcgcca tttcttcatt tatgccgaga
    18901 cgaaacatag cggacgcttt gtcctgaaca atcagacgct gtgcaagtag taaatatgac
    18961 aagttgatgt cataaatgtg tttcagcaac tcggaggtat gcattattcc cacccagaat
    19021 aaccaacttt atttttatgc ggtctcaccg caccccgtga tgtcgccggg aagccccggt
    19081 aaaaaataat tagcattaga atagttgcga taagctgcaa taggcagagc ccctttgtgg
    19141 gatttaataa gtccttacaa atagaagggg ttctttacac ttatctaaga tttttcctaa
    19201 atcgacgcaa ctttactcgt cactacacgc acatacaacg gaggggggct gcgattttca
    19261 ataatgtgtg atgcagatca cacaaaacgc tcaattactt aacataaatg tataagtcat
    19321 acttttgttt tgggtgtatt tcaatctgtt aaaaagtttt tcgcgacgct agcaagcaaa
    19381 aatgaaacag gaataataga aatgggatgt tgcgcacagt taaaataact caccgtaaat
    19441 aatcatctgc tataaata
//
LOCUS       DAZGQL010000091        19448 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.091, whole genome shotgun sequence.
ACCESSION   DAZGQL010000091 DAZGQL010000000
VERSION     DAZGQL010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19448
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.091"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(184..1803)
                     /locus_tag="ACHKIR_003847"
     CDS             complement(184..1803)
                     /locus_tag="ACHKIR_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilN family type IVB pilus formation outer
                     membrane protein"
                     /protein_id="HFO9991525.1"
                     /translation="MNKKILAIMIAAALLQGCASYRQLESTRKEAVAEQQRAREMMSD
                     LRTRTPVVKESSRQWINPRPVMDKNTGKTVPGCPIVIHTRQSITLQQVVQRITESCHI
                     PVRITPDVLTYLNTSTTGSTQQIQGALPPPDASGMVPLAAIGASDTPPSRLQGNTAAL
                     SELNASGLTTLLNAVSSRLGISWRYDGSHIIFYYLETQTFPITYMDSQVAYNATVVSG
                     TMSSNGSSGGSASDAISGDASNTQTTTVDMKSALYQDVKNAVTSMLTPGIGRMFMSTG
                     FITVTDNPQVLETVRAFIEKRNEEMKRQVVLNVEILSIRKTRNEQAGIDWNAVFSDRT
                     LGLSLGSTFTSAASDAITGGVSIVDGKLTGSKAFLKALSSQGDVSVVTQNSAVTKNLT
                     PVPMQIANQQSYIESVTTDTTANVGSSTSLNAATITTGFNMTLLPFILPDSQTLQLLY
                     SMSLSDKPVIENYESGGSKAQLPNVDLKTINQTVDLKSGQTVIISGFQQSGRRSGKQG
                     VGTPGFFGLGGGINSENDDTILVVLITPNII"
     gene            complement(1834..2271)
                     /gene="pilM"
                     /locus_tag="ACHKIR_003848"
     CDS             complement(1834..2271)
                     /gene="pilM"
                     /locus_tag="ACHKIR_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilM"
                     /protein_id="HFO9991526.1"
                     /translation="MGWFIVFIMVITTTGNFWFTSQLSRSEHRHQAAENTQQAATFIR
                     YMNAINDYLHQHPERRTAGGRLTSAQLGIPGTDTVSHIISQQRVFVWATETPGLMAAL
                     REQSNDSALLGRVENGRLLDTAGRALSITLPSVIPDHVILWMN"
     gene            complement(2276..3346)
                     /locus_tag="ACHKIR_003849"
     CDS             complement(2276..3346)
                     /locus_tag="ACHKIR_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000730426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpQ domain-containing protein"
                     /protein_id="HFO9991527.1"
                     /translation="MQKKSLTLLLPSLLSGCTAFNATPVPDTTPPALVFVDGQIRESA
                     AIIAHTQKRLLPPPVTRPVTPPRPTTTATPTTAVSSQSTALPPRPLLQATQLPALVMT
                     GKPGQSSVVYLREARQRSVSDWIRQLLPPGWQFRQQNAAIPALNKRLPVWSVNDQWHR
                     ALNTLLTGQQLYGHMDWNRKILTVTTTAAPPVDLTAPQGSQKTADTPRNPFRSTTGTA
                     ADQAQVPPAVSASRAQSTTGTSGTSAPRTTTPAPVVTKPVAPPPQKVTPPRQWKMEKG
                     TTLKDGLARWASSEKCPHGTWTLRWETPVNYSIDAPLVFTGSFRQALEQLFALYTPVE
                     KPLYARTSTAQCLLRVTDRPQE"
     gene            complement(3503..3838)
                     /locus_tag="ACHKIR_003850"
     CDS             complement(3503..3838)
                     /locus_tag="ACHKIR_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9991528.1"
                     /translation="MRTITTREQLLINGRVHERIATHIVTGAHGYETLCTNGYNVRYN
                     KAQQLVESFEKIAEGKLPVTCPVCFAIWQDVHRFTHVDFDTQSGKSDFIDTCHSEIIT
                     GNVSITGKK"
     gene            complement(3955..4197)
                     /locus_tag="ACHKIR_003851"
     CDS             complement(3955..4197)
                     /locus_tag="ACHKIR_003851"
                     /inference="COORDINATES: protein motif:HMM:NF022090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilI type IV pilus biogenesis protein"
                     /protein_id="HFO9991529.1"
                     /translation="MSAEKFRVVIYTNTCEKKVSYIPATEDINIAIMPFQTADNGLII
                     VCQNSRIIRRKERIPGSSCEWKDVTLAPKSRNRVQL"
     gene            complement(4278..5972)
                     /locus_tag="ACHKIR_003852"
     CDS             complement(4278..5972)
                     /locus_tag="ACHKIR_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000482663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFO9991530.1"
                     /translation="MFNLPGWLFTVIALVLTLFVIGLIFARLYRRASAEQAFVRTGLG
                     GQKVVMSGGAIVMPIFHETIPVNMNTLKLEVSRAAAESLITRDRMRVDVAVAFFLRVK
                     PSAEGISTAAQTLGQRTLTPEDLRSLVEDKFVDALRATAARMSMQDLQDARENFVQGV
                     QNTVAEDLSKNGLELESVSLTSFNQTAREHFNPDNAFDAEGLTLLTQETERRRRERNE
                     VEQDVEVAIREKNRDALSRRLEIEQQEAFMTLEQQQRVKTRTAEQSASIAAIEAERRR
                     EAESARILAERKIEEAEIERQQIVRTRQVEAEREVAIREIEQQQATEIASQARAIAVA
                     AKSEEQSQAEARASKALAEAVQAQQDVETTRQTAEADRTKQVALIAAAQEAETKAVEL
                     TVRAQAEKEAAELQAAAIIELAEATRQKGLAEAEAQRALNDAINVLSDEQTSLRFRLA
                     LLDALPAVIEKSTEPMKSIEGIKIIQVDGLNGGVTRGKTATTDISGGNLAEQAVTAAL
                     AYRTQAPLIDSLLKEVGLSGEGLTALTRPLVMPAPEKVPDVSEPVTAPDNSEPQQV"
     gene            complement(5999..6619)
                     /locus_tag="ACHKIR_003853"
     CDS             complement(5999..6619)
                     /locus_tag="ACHKIR_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HFO9991531.1"
                     /translation="MTLTLLTAYNAPYLAALTFVLLTGIAELIALLCGHSLSSAMDTP
                     DLPEGLTGEALDWLNIGRIPLLIVLCMLAVFFGISGILLQGLIIHLLQAPAPNILLAP
                     LCLLLTCPLVHRTGRLIAPWLPRDETTALTEEAFIGCMATITGYHAVAGSPCEGRFTD
                     RFGQVHYLLLEPEAGKEFRRGDKVIIVCRLSGSRWLGELNPWPDVL"
     gene            complement(6885..7550)
                     /locus_tag="ACHKIR_003854"
     CDS             complement(6885..7550)
                     /locus_tag="ACHKIR_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000154135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="traC"
                     /protein_id="HFO9991532.1"
                     /translation="MTILSQENPVILLLENMLEALSTAPDNINNERRRRRYLLNWLDT
                     ARQMREFRGMAEEFTTLRKLLATNSDIPVANTLKSLLESGNNAALYDLYRFRSALSAC
                     RSLGWQIGTCAWNDQLLSETLSRAQTGKRHILQLNSTRDTFSTTGRMLRPVAFSLIHP
                     ASLESSEVEEIFRDEGFILAHGRECSLNGSGRNMLSRILHVGHSGLPKAEWGIDHSNH
                     LLH"
     gene            complement(7691..8332)
                     /locus_tag="ACHKIR_003855"
     CDS             complement(7691..8332)
                     /locus_tag="ACHKIR_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0140673 - transcription elongation-coupled
                     chromatin remodeling [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HFO9991533.1"
                     /translation="MCLSELQAGLNEGQPLFPTHALCIRRSQQHRSPSAGIDFRGAFR
                     ILNISELHQRNWYLAQYIPAGKNREHLFSWLSEQHVLPWTPLILKKVRRTDKVCGYRR
                     HIHAVFPGYFFLKADPERHSFTHLRRHSAFLNFVKMAGEIKTVREDIVQSLMKVYPDP
                     ALNPAAREELDAASTLWLTKARYQYLLRLDAQPLPESRIALLLHLVSDDGALT"
     gene            9035..9907
                     /gene="repA"
                     /locus_tag="ACHKIR_003856"
     CDS             9035..9907
                     /gene="repA"
                     /locus_tag="ACHKIR_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HFO9991534.1"
                     /translation="MAALNQTSSTESTYRQVKNPNPVFTPREDAGTLKFCEKLMEKAV
                     GFTSRFDFAVYVAYARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTL
                     AIECGLATESAAGKLSITRATRALTFLSELGLITYQTEYDPLIGCYIPTDITFTPALF
                     AALDISEEAVASARRSRVEWENRQRKKQGLDALGMDELIARAWRFVRERFRSYQAELK
                     SHGMKRARARRDAGRTRQDIVTLVKRQLTREIAEGRFRGSLEAVKREIDRRVKERMIM
                     SRNNNYTRLATASP"
     gene            10814..11083
                     /gene="yacA"
                     /locus_tag="ACHKIR_003857"
     CDS             10814..11083
                     /gene="yacA"
                     /locus_tag="ACHKIR_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015056448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin YacA"
                     /protein_id="HFO9991535.1"
                     /translation="MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQ
                     HNTWFRDQVAAGRQQLEHGDVLPHDMVESSAAAWRDKMSRKVAGK"
     gene            11083..11361
                     /gene="yacB"
                     /locus_tag="ACHKIR_003858"
     CDS             11083..11361
                     /gene="yacB"
                     /locus_tag="ACHKIR_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin YacB"
                     /protein_id="HFO9991536.1"
                     /translation="MEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAG
                     QPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVYILRVLHTAQKWP"
     gene            11407..12255
                     /locus_tag="ACHKIR_003859"
     CDS             11407..12255
                     /locus_tag="ACHKIR_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFO9991537.1"
                     /translation="MNNDELATRRAQAIAEDRCFSKGRLRDEFRMKPAPGAEPVKWYK
                     NTYGGRFAVYRIADCVPMREKRPLTSKQLLAGQRLSVLSRLNSTSGRMARQAYDWLSL
                     APLFLDTETTGLDNTAEALEIGLTDAAGQVVFETRLKPTVAIGAQAAAVHGISEQALC
                     GAPSWTDVARQLRHAIGGRPVIIFNARFDIRILKQTAAAHSDPAYWLEEMTVYCAMEL
                     AAGYYGATNRYGTISLASAASQAGLTWEGLAHSAIADARMTAGVVNAIAAYHLELLQE
                     QARLEI"
     gene            12396..12854
                     /locus_tag="ACHKIR_003860"
     CDS             12396..12854
                     /locus_tag="ACHKIR_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001236005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9991538.1"
                     /translation="MRLFLGGRCYTAEKLEKDYLAEVANYSNDRWEAPQRASRLAASV
                     KRYKTSEMLRFIFATIAYDPDPDLTPLTVRRLCKALFGRTGSQWLVVEVFGEKGRQHR
                     SADSNPEMVEKMAARYRHAAELHWSATLAEIERVKRLYQTKIKKSKKEVG"
     gene            complement(13218..13643)
                     /gene="nudI"
                     /locus_tag="ACHKIR_003861"
     CDS             complement(13218..13643)
                     /gene="nudI"
                     /locus_tag="ACHKIR_003861"
                     /EC_number="3.6.1.12"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HFO9991539.1"
                     /translation="MRQRVIVCPVIQNEGEFLLCKMASDRGVFPGQWALPGGGMEPGE
                     TMETALRREIKEELGKELHITDVKPWAFRDDIRTKTYPDGTHEDIYMIYLIFDCISTN
                     RTVTFNEEFQDIVWASPDTLKYMDLNEATRITFSQKGIL"
     gene            complement(13645..14352)
                     /locus_tag="ACHKIR_003862"
     CDS             complement(13645..14352)
                     /locus_tag="ACHKIR_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014727861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFO9991540.1"
                     /translation="MRYNYLYMDPWSADKKEKNKTRNPLYICLSGNSGVGKSTLLRRM
                     SKELFKVDPDTVAIDEKSVHHTLLPYLFDDTSNYGYLIQLNFMIQRALLIKSWLDKGF
                     NLIMERSHIEDYIFINFMLKSEYINKAQYDAYMVLWSRINELLTEPDIIAFINYPIAH
                     SLMHLNDDEIHGVRPEEFPDEEMKIKWITGWHAEYQHFTDNLPLHLRERIMVCAHPDD
                     IDDFSDKVIQKAMMLRT"
     gene            complement(14349..15215)
                     /locus_tag="ACHKIR_003863"
     CDS             complement(14349..15215)
                     /locus_tag="ACHKIR_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFO9991541.1"
                     /translation="MDISTLLLDFRITSHCNLGCDLCFRTPGIQDSSLASARNVIERM
                     YKMGFRRIGFTGGEPTSRSDFIDLIEYAKQLGFMTYLSTVGHRFIMDHARLNPVLDWV
                     GIPIDGIDSLVNSEIRSDKMSNQHRVIKNILTWLPQNNNRINIKLTTVVSQSNINHLS
                     DIVCWVDSLPYKIQAWRFYQFCPLGVGKEKRDKLEINTDIFKDKMVMLKKQHPNMPIS
                     WATFEERDKANVVMEPNFDIIIPDGENYTLLGNMLTDSQDHIISAIFGNNEIMQKCKN
                     NRFWLDKMEFTV"
     gene            15700..15951
                     /locus_tag="ACHKIR_003864"
     CDS             15700..15951
                     /locus_tag="ACHKIR_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001595120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFO9991542.1"
                     /translation="MPNIILSDISASVSELKKNPMATVGAGDGYPVAILNRNQPAFYC
                     VPAELYERMLDALDDQELVKLVTERSSQPLHDVDLDSYS"
     gene            15948..16235
                     /locus_tag="ACHKIR_003865"
     CDS             15948..16235
                     /locus_tag="ACHKIR_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO9991543.1"
                     /translation="MTYTIKFRDDALKEWLKLDKTIQQQFVKKLKKCSKNPHILSAKL
                     RGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGKRERSNVYYLASERMR"
     gene            16403..16807
                     /locus_tag="ACHKIR_003866"
     CDS             16403..16807
                     /locus_tag="ACHKIR_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016529521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2251 domain-containing protein"
                     /protein_id="HFO9991544.1"
                     /translation="MTILVTAQAQLIVGEAMAIKSLAPDGMLAAVFEDDGNTGYFYAL
                     DESVEGNPVRDAVHIYNVEDISDAHIPSDVKIGWSEDCLKCVLLINGYPHGVFDFEGK
                     NGYCRSGFPPPVNHEWSVQGHSWDDYVDNFFR"
     gene            17116..18600
                     /locus_tag="ACHKIR_003867"
     CDS             17116..18600
                     /locus_tag="ACHKIR_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO9991545.1"
                     /translation="MRKIIAHFKIVLTLLLPATVSAQSIEWQSCMTTPFSNWFGEEKP
                     SPDLLCGYLSVPLKYTDTGGDSSSVKRQQVKLALTKLPAKSRHKGSIIIISGGPGLPG
                     INPYINFDWPVTNLRESWDIIGFDPRGVGQSIPAINCQQSDTGIQENITEKQRVLNKI
                     NSCIHNTGTEVIRHIGSNEAVYDIDRIRQALGDEQLTAVAYSYGTQIAALYAERFPSS
                     IRSIVLDGVVDIDDLGDNFTWQLKQAQSYQETFDRFASWCARTKSCPLSSVRDKAITQ
                     FHELLSKLHHKPLLDSKGENISSDELILLTTDLLLWRSSWPTLATAIRQFSQGIVSNE
                     IETALSTPIASEESSDALGVILCVDQGGEQLTPEERKSRKDALANAFPAINFDNGRSD
                     SPDFCELWPIHSNLNKTRLKNTVLPSGLLFVAHKYDPTTPWINARKMAEKFSSPLLTI
                     NGDGHTLALTGVNLCVDKAVVRHLITPMKMGNISCPGNAEIDIK"
     gene            complement(18821..19429)
                     /locus_tag="ACHKIR_003868"
     CDS             complement(18821..19429)
                     /locus_tag="ACHKIR_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HFO9991546.1"
                     /translation="MGKDLEEKKNPVIVVKKRRSFSPPSLAEKKDVIAPALTEQATES
                     VSSGISSPAVETHIPEAPARKKKKKRQRFPRPSHWTSEFTHDCLEKVKALFPQLRAEG
                     GGFLPLKTGITRDITTYLAAHPETALTLDEWNCAVSCITSRRIYLQRTSVAGTPRYNL
                     DGQPEGEVSECDAQNSRRWLAAKEQRWLSMKARQENISEKSK"
BASE COUNT         4849 a         4561 c         4984 g         5054 t
ORIGIN      
        1 gatactgaca ggccaggaaa aggtcggctt tctgtgcccg ggcaaagtcg cgggcatgcc
       61 ggtactgtga gggcagtgtt ttccattcca gaccggccag aaacacattg cgccctgccg
      121 tcagcggaaa cacctgccgc cctgtcggtg aaatatccgt cacgatcgtc ctccttgtgc
      181 aaatcagatg atattgggcg tgatcagcac cacgagaatg gtgtcgtcgt tttcactgtt
      241 gatcccaccg ccaagaccga agaatcccgg cgtgcctaca ccctgttttc cgctgcgacg
      301 tcctgactgc tggaagccac tgataatgac cgtctgcccg gatttcagat ccacggtctg
      361 attgatggtt ttcagatcca cattcggcag ctgggcttta cttccaccgc tctcataatt
      421 ctcaataacc ggtttgtcgg agagactcat ggaatagagc aactgcagtg tctgtgagtc
      481 cgggagaatg aacggcagaa gggtcatgtt aaaaccggtg gtgatggtgg ccgcattcag
      541 ggaggtggat gagccgacat tggcagtggt atccgtcgtc acactctcga tgtaactctg
      601 ctgattggcg atctgcattg gcaccggcgt caggtttttg gtgacggcag agttctgagt
      661 caccacactg acatccccct gggaagacaa cgctttcagg aaggcttttg aaccggtgag
      721 tttaccgtcc acaatggaaa caccacctgt gatggcatca ctggctgcac tggtgaatgt
      781 gctgccgaga ctcagtccca gcgttctgtc gctgaagact gcgttccagt cgataccggc
      841 ctgctcattg cgggttttcc ggatactcag aatttccaca ttcagcacca cctgacgttt
      901 catctcttca ttgcgttttt caatgaatgc ccggaccgtt tccagaacct gtggattatc
      961 tgtgacggtg ataaagcccg tggacatgaa catgcgtccg atacccgggg tcagcattga
     1021 tgtgaccgca tttttcacat cctgatacag cgcagacttc atatccacgg tggtggtctg
     1081 ggtattgctg gcatcaccgg aaatggcatc tgatgcactg cctcctgatg agccgtttga
     1141 ggacatggtg ccgctgacca cagtggcgtt ataggccacc tgactgtcca tataggtgat
     1201 ggggaaggtc tgtgtctcga gataatagaa gatgatatgc gagccgtcat agcgccagga
     1261 tataccgaga cgggaggaga ccgcattcag cagggtcgtc aggccggagg cattcagttc
     1321 agacagtgct gcggtatttc cctgcagacg ggatggtggc gtgtcagatg cgccaatggc
     1381 ggcaagggga accatgccgc tcgcatccgg tgggggtaac gcgccctgga tttgttgtgt
     1441 gctgccggtt gtggatgtat tcaggtatgt cagtacatca ggtgtgatcc gtaccggaat
     1501 atggcagctt tccgtgatcc gctgaaccac ctgctgaagg gtgatgctct gacgggtatg
     1561 aatgacaatg ggacaaccgg gaacggtttt gccggtgttt ttatccatga ccggacgggg
     1621 attgatccat tgtcgtgagg attccttaac aaccggcgtc cgggttcgca gatcagacat
     1681 catttcccgt gcccgttgtt gttccgcaac ggcttccttt cttgtgctct caagctgtct
     1741 gtaactggca cagccctgta acagagcggc ggcaatcata atggcaagaa tttttttatt
     1801 catgggagat ccgtgattta ttcagattcg tgactaattc atccagagga tgacgtgatc
     1861 ggggatcact gacggaagtg tgatggaaag cgctctgccg gcagtgtcca gcagtcggcc
     1921 gttttccacc ctgccgagca gagcagagtc attactctgt tccctgagcg cggccatcag
     1981 gcccggggtt tctgtggccc agacaaaaac acgttgctgg ctgatgatat gactgacggt
     2041 atccgtcccg ggaatcccga gctgagcgga tgtcagccgc cctccggctg tccgtcgttc
     2101 cggatgctga tgcaggtaat cattgatggc gttcatataa cgaatgaacg ttgctgcctg
     2161 ttgtgtgttt tctgcggcct gatgacggtg ttcgctgcgg gagagctgac tggtgaacca
     2221 gaaattgcct gtggtcgtga tgaccatgat gaacacgatg aaccagccca taaggttatt
     2281 cctgtggacg atctgtcacc cgcagcagac attgtgctgt gcttgtccgg gcatacagcg
     2341 gtttttccac cggggtgtag agtgcgaaga gctgttccag cgcctggcgg aaactgccgg
     2401 tgaaaaccag aggggcatcg atactgtagt taacgggcgt ttcccagcgt agcgtccagg
     2461 tgccgtgggg gcatttttcg gacgacgccc agcgggccag tccgtccttc agcgtggtcc
     2521 ctttttccat tttccactgc cgtggcggtg tgaccttctg tggtggtggc gctaccggtt
     2581 tcgtgaccac cggggctggc gtggttgtgc ggggcgcaga tgtaccggat gtccctgttg
     2641 tgctctgagc ccgggaagcg ctgacggcag gtggtacctg tgcctggtct gctgccgttc
     2701 cggttgtgct cctgaacgga ttacgtggtg tgtccgctgt tttctgtgaa ccctgcggtg
     2761 ccgtgaggtc aacaggcggt gctgcagtgg tcgtgacagt cagtattttc ctgttccagt
     2821 ccatatgtcc gtacagttgc tgcccggtca gcagagtatt cagtgcccgg tgccactggt
     2881 cattaacgga ccagacgggg aggcgtttgt tcagagccgg tatggcagcg ttctgttgcc
     2941 ggaactgcca gccgggtggc agcaactggc gtatccagtc actgacggag cgctgacgtg
     3001 cctcacgtaa ataaacgacg gatgactgac cgggtttacc ggtcataacc agtgcgggca
     3061 gctgtgtggc ctgaaggagc gggcggggag gcagtgcggt actctggctg ctgactgcgg
     3121 ttgtgggcgt cgctgttgtt gtcgggcggg gcggcgtaac agggcgggtc accggtggcg
     3181 gaagcagacg tttttgcgta tgggcgataa tggcagcgct ttcgcgaatt tgtccgtcga
     3241 cgaacaccag agccggaggt gtggtgtcag gaacaggcgt ggcattaaat gccgtacagc
     3301 cgctgagtaa tgacggcaga aggagtgtaa gtgatttttt ttgcatagaa taaaatccgg
     3361 tatattacgg gttacggtat aagacaggtg gcgggtaact ggccttcaga tgaatatcat
     3421 ttcatcgggt tattgataat gaacgttcat gttcgtgggg ggattataag agtgaattgt
     3481 attttagaac gggagcatta ttttatttct tgcctgttat tgaaacattt ccggttatga
     3541 tctccgaatg gcaggtatca ataaaatcac ttttccccga ttgtgtgtca aagtcaacgt
     3601 gggtgaacct gtgtacatcc tgccatatgg cgaaacagac aggacaggta acaggtagtt
     3661 tgccttcagc tattttttcg aagctttcaa ccagttgctg cgccttgttg tatctgacgt
     3721 tatagccatt tgtgcaaagc gtttcatacc catgagctcc tgtaactata tgtgtggcga
     3781 ttctttcgtg gactctccca ttaattaata attgttcacg cgttgtgatt gttctcatgc
     3841 ctgtttctct gttggtcata acataaaata atctgcattc ctgaaaaata tcgcggttgt
     3901 gaaatattta atttttatcc cccttttatg ggggataaaa agcaggctgt caggctataa
     3961 ctgaacgcgg tttctggatt ttggtgctaa tgtcacatct ttccattcac agctgcttcc
     4021 gggtatacgc tctttgcgtc gtattatacg ggagttctga cacacaataa ttaagccatt
     4081 gtctgccgtc tgaaatggca ttatagcgat gtttatatct tctgttgcag gaatataact
     4141 gactttcttt tcacatgtat tggtgtatat gacaactctg aatttttctg cggacatttt
     4201 caatcctctt ttaaaccaga atatcctgtg agggcattct ttgtgaaaaa gatgccggaa
     4261 aaattccggc acggggatta aacctgttgt ggttctgagt tatccggagc ggttaccggt
     4321 tcgctgacat ccggaacctt ttccggggct ggcatgacca gtggacgtgt cagggctgta
     4381 agtccttcac cggataaccc cacctccttc agcagggagt cgatgagcgg agcctgtgta
     4441 cggtaagcca gagcggcggt taccgcctgt tcagcgagat ttccgccact tatgtcggtt
     4501 gttgctgttt taccacgggt aacaccgcca ttcaggccat ccacctgaat gattttaatg
     4561 ccttcaatgg atttcattgg ttctgtggac ttctctatga cggcaggtaa tgcatccagc
     4621 agggcaagac ggaaccggag gctggtctgc tcatctgaaa gcacgttaat ggcatcgttc
     4681 agtgcgcgct gtgcttccgc ttctgccaga cctttctgac gggtggcctc cgccagttca
     4741 ataattgctg ctgcctggag ttcggctgct tctttttcag cctgggcgcg aaccgtcagt
     4801 tcaaccgctt tcgtttcagc ttcctgagca gcagcaatca gggcaacctg tttggtacgg
     4861 tcggcctccg cagtctggcg ggtggtttcc acatcctgct gggcctgtac ggcttcagcc
     4921 agtgctttac ttgcccgggc ttctgcctgt gactgctctt ccgattttgc cgccacggca
     4981 atggctctgg cctggctggc gatttcggtt gcctgctgtt gctcaatttc ccgtatggcc
     5041 acttcccgtt cggcttcgac ctggcgggta cgcactatct gctgtcgttc gatttcggct
     5101 tcctctatct tacgttcagc cagaattcgc gcggattctg cttcacgacg tcgttcagct
     5161 tcaatagcag caatactggc actctgctct gccgtacggg tttttacccg ttgttgttgc
     5221 tccagagtca tgaaggcttc ctgctgttca atctccagac gtcgggagag tgcatcccgg
     5281 tttttttccc tgatggccac ttcaacatcc tgctcaactt cgttacgttc gcggcgacga
     5341 cgttctgttt cctgggtcag aagggtcagg ccttcagcat caaaggcatt gtcaggatta
     5401 aaatgttccc gtgcagtctg gttgaaactt gtcagcgaca cactttcaag ctccagaccg
     5461 tttttggata agtcttccgc aacggtgttc tgcacaccct gaacaaagtt ctcgcgggca
     5521 tcctgtaaat cctgcatgct cattctggcc gccgtggccc gcagggcatc aacaaattta
     5581 tcctcgacca gactacgcag atcttcaggt gtgagcgtgc gttgtcccag cgtctgggct
     5641 gcagtggata ttccttcggc tgatggtttt acccgaagaa aaaaggcaac ggcaacatca
     5701 acacgcatgc ggtcgcgggt aatgagactt tcagccgctg cacgactgac ttccagcttc
     5761 agggtattca tgttgacggg aattgtttcg tgaaaaatgg gcataacaat tgcaccgcca
     5821 ctcattacca ctttttggcc tcccagacct gtgcggacaa aggcctgctc tgccgatgcc
     5881 cgacggtaca ggcgggcaaa tatcagcccg ataacgaaca gggtgaggac cagcgcaata
     5941 acggtaaaca accatcccgg aagattaaac atagtgagaa cctctgtata tgtcgggatt
     6001 aaagaacatc cggccaggga ttcagttcac ccagccagcg ggaaccggaa agacggcaga
     6061 caataatcac tttgtctcct cgtctgaatt ctttcccggc ctccggctcc agcaacaggt
     6121 aatgaacctg cccgaagcgg tctgtgaagc gaccttcaca gggggaaccc gccactgcgt
     6181 gataaccggt gatggtggcc atacagccga tgaatgcctc ctcggtgagc gccgttgttt
     6241 catcacgggg taaccagggg gcaatcagtc tgccggtgcg gtgtacgaga ggacatgtca
     6301 gcagcaggca aagtggtgca aggagaatgt tcggggcagg agcctgcagc agatgaataa
     6361 taagaccctg caggagaatg ccggatatac caaaaaaaac tgccagcata cagaggacga
     6421 tcagcagggg gatccgtccg atgttcagcc agtccagcgc ctctcctgtc agaccctccg
     6481 gtaagtcagg tgtatccata gctgaagaga gggagtgtcc gcagagcagg gcgataagtt
     6541 cagcgatacc agtaagcaga acaaaagtca gggctgccag atagggtgcg ttatacgcgg
     6601 taagtaacgt cagtgtcatt tttcctcctg tggcaggtca gtcaaacaac attatgcgtg
     6661 aagcttccgc ttctttaatt gtcagaagaa gcatatcgtc agtcatcaga agacaagggg
     6721 atgtctgcag gaggggatga taacaacaga agcctcacgc cggaatgtgc tttatgacaa
     6781 caggagagga cggataataa aaaatgggac atccacatca gcaaaccgcg atttgcgaaa
     6841 aaaagtcagc ggagagtggc tgccgttgtg gctgtcccgg tatttcagtg taacaggtga
     6901 ttgctgtgat ctatgcccca ttcggctttc ggtaaacctg aatgccccac atgaagaatg
     6961 cgtgacagca tgtttctgcc gctgccgttc aggctgcatt cgcgtccgtg cgcgagtata
     7021 aaaccttcat cccggaatat ctcttccact tctgaagatt cgagggatgc cggatgaatt
     7081 aatgaaaatg ccaccggtct gagcattctg cccgtggtgg aaaaggtgtc tctggtactg
     7141 ttcagctgca ggatatgacg tttacctgtt tgcgcacggg aaagtgtctc actcagcagc
     7201 tggtcgttcc atgcgcacgt gccaatctgc catcccagac ttctgcacgc ggaaagagcc
     7261 gagcggaagc ggtacaggtc gtataatgct gcgttattgc cactttccag cagtgatttc
     7321 agggtgtttg ctaccggtat gtctgaattg gtggccagca gttttcgcag ggtagtgaac
     7381 tcttcagcca ttcctctgaa ctcgcgcatc tgacgggcag tgtcaagcca gttcagcagg
     7441 taacgccggc gacgccgttc gttgtttatg ttgtccgggg cggtggagag tgcttcgagc
     7501 atattctcca gtaacagaat gacagggttt tcctgggata gaatggtcat aaaggcgtcc
     7561 tgaaaaaatg cccgacagcc gctctccgta caccctcagg tgtcaccggt gccgggcatt
     7621 gtggaaacag aagggaaaaa aatcccggcc ataaaggccg ggaaaagtcg tcaaattaag
     7681 acagcaattt tcatgtcaac gctccgtcat cagataccag gtgcaggagc agggcgatcc
     7741 tcgactccgg cagaggctgt gcatcaagcc ggaggaggta ctggtaacgc gcttttgtca
     7801 gccagagggt ggaggcagca tccagctcct ccctggctgc cggattgagc gcaggatccg
     7861 ggtacacttt catcaaagac tgaacgatat cctccctgac ggttttgatt tcaccggcca
     7921 ttttcacgaa gttcagaaag gcactgtgac ggcgaaggtg agtgaagcta tggcgttcgg
     7981 ggtctgcttt caggaaaaag taaccgggaa acacggcgtg tatatgtcga cggtaaccgc
     8041 agactttatc cgtgcggcgg acttttttca gtatcagggg tgtccaggga aggacgtgtt
     8101 gttcggaaag ccaggaaaag agatgttcac ggtttttgcc ggcagggatg tactgagcca
     8161 gataccagtt gcgttgatgt aattcactga tattcaaaat ccggaacgct ccgcgaaaat
     8221 cgatccccgc agaggggctt cgatgttgtt gtgaacggcg aatacataaa gcatgtgtcg
     8281 gaaacagcgg ctgaccctcg ttcagcccgg cctgcagttc tgacaggcac atgatttacg
     8341 cactcttgta agccttgtgg acggtttcga ggcgggcgaa aaccgacaac ttctgcaaac
     8401 aggattaatg gctggcaaat atattacata aggatgtatc tgtggcaaga gtgaagataa
     8461 gcagttaaat agatcgttat atttaataaa tcagcatatt atgctgattt gtgtgcggtg
     8521 tctggaggat tagatcgttt aaaaatttta ggcgatctct tctactaaaa atgatgaaaa
     8581 acacagcata aaagatcgtt tgtggaattg tctatttttt tgacatagtt caaaaaaacg
     8641 acgtttttta ggtggttaat gagaggcctg gtaaatcaga gaaatcgtgt cggcttttca
     8701 aagcggtgga aaaggggtat attgcggatc gttatgcagt ggcttttggg atcgtcagga
     8761 tccgggaagt cagaaaacgg caggatgcgc cataaggcat tcaggatgta tggcagaaac
     8821 gacggcagtt tgccggtgcc ggaaggctga aaaaagtttc agaagaccat aaaggaaaac
     8881 ccccataatc ttcttatcga acttggcgga acgtagagaa gattatgggg gcctcacaga
     8941 atacgggata agtatatatg aaaccgtacc agagattcaa cccttcgcag tgcattaacg
     9001 cacggcattc tgtttccgcc ataagcgaca gcttgtggcg gccctgaacc agacctcatc
     9061 tactgaaagc acataccgtc aggtaaagaa cccgaatccg gtcttcacac cccgtgaaga
     9121 tgccgggacg ctgaagttct gcgaaaaact gatggaaaag gcggtgggtt tcacttcccg
     9181 tttcgatttc gccgtgtatg tggcgtatgc ccgttcccgg gggctgcgtc ggcgcatgcc
     9241 accggtactg cgccgacgtg ccattgatgc gctgctgcag gggctgtgtt tccactatga
     9301 cccactggcc aaccgcgtcc agtgttccat caccacgctg gccattgagt gcggactggc
     9361 gacggagtct gctgccggaa aactctccat cacccgtgcc acccgggccc tgacgttcct
     9421 gtcagagctg gggctaatta cctaccagac ggaatatgac ccgcttattg gctgttatat
     9481 tccgaccgac atcacgttca caccggcact gtttgccgcc ctcgatattt ccgaagaggc
     9541 ggtggcctcc gcacgccgca gccgcgtgga atgggaaaac aggcagcgca aaaagcaggg
     9601 gctggatgcg ctgggtatgg atgaactgat agcgagggcc tggcgttttg tgcgcgagcg
     9661 tttccgtagt tatcaggcag agcttaagtc ccacggcatg aaacgtgccc gtgcgcgccg
     9721 tgatgcaggc agaacccggc aggacattgt caccctggtg aaacggcagc tgacgcgtga
     9781 aatcgcggaa gggcgtttcc ggggaagcct ggaggctgtg aagcgcgaaa tcgaccgtcg
     9841 cgtgaaggaa cgcatgataa tgtcgcggaa caacaactac acacggctgg ccacagcctc
     9901 accctgagtt ttacctcatc tgaataatcc ggcccgcgcc ggaggcttcc gcacgtctga
     9961 aggccggcga cgcacaaaaa agcagcgcag cacacaaaaa acagcctcat catctgcctt
    10021 ccggctcgcc ttgctccccc tgtttttcac gcaaaactcg tctcacagac gggggattct
    10081 gcttatccac atttaactgc aagggacttt tccgtaaggt tacaaccgct cattccataa
    10141 agcgccagcc gcaggcgtta cagggtgcaa tgtttctttt aaaccccagt actgtatttc
    10201 ctttaaacta cttaattagt tccatttaaa aagaaaacct attcaatgcc tgtcctgtgg
    10261 acaggcaaat atgtacctcc cactctaacg cgcgggcccc gaacctcacc gttctgaaac
    10321 cacagcaaaa aaacatcagg aataaaaaca ctacacaaac gcagcaccgt gcccacccct
    10381 cataactgaa aagcgaggcc gcccccgccc aaagggcggg aacaacatcg cttttaatta
    10441 tgaatgttgt aactaaacaa caccatcgct gtcagtcttc tggctggacg tactgaatac
    10501 acgctcgtaa gcggccctga cggcccgcta acgcggagat acgccccgac ttcgggtaaa
    10561 ccctcgtcgg gaccactccg accgcgcaca gaagctctct catggctgaa agcggatatg
    10621 gcttagcagg gtgggaatgg gataggcgaa atctatcaat cagtaccggc ttacgccggg
    10681 cttcggcggt tttactccgg tatcatatga aacaatggag tgccgccttc catgccgctg
    10741 gcgcggcata tcctggtgac gatatctgaa tcgttatata ctgtgtatat acgtagtaat
    10801 gacgaggtga taaatggcac aggttaatat gagtttaaga atcgacgctg aactgaagga
    10861 tgcttttatg gccgctgcaa aaagcatgga ccgtaatggc tctcagttaa tccgggattt
    10921 tatgcgccag accgttgaac ggcagcataa tacctggttc cgcgaccagg ttgcggcagg
    10981 acgtcagcaa ctcgagcacg gcgatgtgct tccccatgac atggtcgaat cctctgctgc
    11041 agcatggcgg gataaaatga gcaggaaggt cgccggtaaa tgatggagat attctggact
    11101 atgctggcca gccaggacag gaagcgcatt cgtgagtaca ttgcggagca gaacctgatg
    11161 gccgccattg aactggacga acggattggt tattcagcca gcagcctggc cgggcagccg
    11221 tataaaggcc gcaacggtcg tgtggaaggt acccgggaac tggttattca cccgcatttt
    11281 gttctggttt atgaggtcga cagccagtgg ggaaaagtgt atatcctgcg tgtattgcat
    11341 actgcacaga agtggccata gagaggctgt ccggatataa aaacgtctga cggagatgca
    11401 agaaccatga ataatgatga gctggccacc agacgtgcgc aggcgattgc tgaagacaga
    11461 tgtttctcga aaggacgtct gcgcgatgaa ttcagaatga agcctgcccc cggtgctgag
    11521 ccggttaagt ggtataaaaa cacttatggt ggcaggttcg ctgtataccg aattgcagat
    11581 tgtgttccga tgcgtgagaa acgcccactg acgagtaaac aacttctggc tggccagcga
    11641 ctttccgttt tatccagact taacagcacc agtggccgga tggcacggca ggcatatgac
    11701 tggctgagtc tggcaccgct ctttctggat actgaaacga caggactgga taataccgcg
    11761 gaagccctgg aaatcggcct tactgatgcg gccggtcagg tggtatttga aaccagactg
    11821 aagcccacgg tcgccatcgg ggcgcaggcc gccgctgtgc atggtatcag tgagcaggct
    11881 ctctgcggcg caccatcatg gacagatgtg gcgcggcaac tgcgtcatgc tatcgggggc
    11941 cgaccagtaa ttatctttaa tgcccggttc gacatccgca ttctgaaaca gactgctgcc
    12001 gcacatagcg atccggctta ctggctggaa gaaatgacgg tatattgtgc gatggagctg
    12061 gcagcaggat attatggggc caccaaccgc tatggcacga tttcactggc cagtgctgcc
    12121 agccaggccg gactgacctg ggaagggctg gcacactcag cgatcgccga cgcacggatg
    12181 acggcagggg tggtaaacgc tattgctgca tatcatctgg aactgctgca ggaacaggca
    12241 cggctggaaa tctgaccgcc tggcctgtat accgcaatca tttacgttat ccggacgtaa
    12301 atctgccgct tgttgctgtc agcatgttaa tgctaatgta tgcgtacatt agcattaatg
    12361 agggatgata tgtgagagac aaaaccaggg aggccatgcg gcttttttta ggcggcaggt
    12421 gctatacggc tgaaaaactt gaaaaggatt atctggctga agtggctaat tacagtaacg
    12481 accgctggga agcaccgcaa cgtgcctcac gcctggctgc cagcgtaaag cgttacaaga
    12541 catcagaaat gctgcgcttt atttttgcta ccattgccta cgatcccgat ccagatttaa
    12601 ccccgctgac tgtgagacgt ttgtgcaagg cattgttcgg cagaaccgga agccagtggc
    12661 tggttgttga agtttttggg gagaaggggc gtcagcaccg cagcgcggac agcaatccgg
    12721 agatggtgga aaaaatggct gccagatatc gtcatgccgc cgaactacac tggtccgcaa
    12781 cgctggcaga aattgaacgg gtaaagcggc tttatcagac gaaaattaag aaatccaaaa
    12841 aagaagtggg ttgactgcca ttgttattct tctccttcat cattaatgct aatgtatgca
    12901 tacattagca ttaatgatgg tgcgttgaac gtacaataag gggtccgctt ggaattcgga
    12961 aattttccca cgctaagaga attgagttga acgagtggtt acatccaggc cagctaactc
    13021 actgaacgta aagactgtac gaaatgttcc gaccgtccga taaccatttg gcttacttcc
    13081 gtacatttcc ttgcgtagat gtacagaatt gtacgttttc gtacggttgg ccctcaaacc
    13141 ccatccttga gtagtggcat aagaaaacgg ctccctgttg tggaagccgt tatcagttgc
    13201 acgattagca ttggagttta aaggatacct ttctgagaaa atgtgatccg ggttgcttca
    13261 tttaaatcca tatattttag ggtgtcagga gatgcccaga ctatatcctg aaactcctca
    13321 ttaaatgtaa ctgtacggtt tgtactgata cagtcgaata ttaggtaaat catgtaaatg
    13381 tcctcgtggg taccatccgg gtacgttttt gtacggatat catcacgaaa ggcccagggt
    13441 tttacgtcag tgatatgcag ttcttttccc agttcttcct ttatttcacg cctcaatgct
    13501 gtttccatag tctctccggg ttccattcct ccaccgggca gtgcccattg gcccgggaat
    13561 actccgcgat cagaggccat tttacaaagt agaaactcac cttcattttg tatcacaggg
    13621 cacactatta ctctctggcg cattttatgt cctcaacatc atggcttttt ggataacctt
    13681 atcagaaaaa tcatcaatgt catccgggtg agcacatacc ataatccgct cacgaaggtg
    13741 taacggaaga ttatcagtaa aatgttgata ttccgcatgc catcctgtga tccattttat
    13801 cttcatttcc tcatctggaa attcttcagg ccgtacacca tgaatttcat catcattgag
    13861 atgcatgagt gaatgtgcga tgggataatt aatgaatgct ataatgtctg gctctgtaag
    13921 taattcattt attctactcc agagcaccat gtatgcatca tattgtgctt tatttatata
    13981 ttcggacttc agcatgaagt tgataaaaat ataatcctca atatgggatc tctccataat
    14041 aaggttaaac cctttatcca gccaagattt tatcagtaat gctcgttgta tcataaaatt
    14101 aagctggatt agataaccgt agttactggt atcgtcaaaa aggtaaggaa ggagagtgtg
    14161 gtggacactt ttttcatcta tcgcgacagt gtctggatct accttaaata actcctttga
    14221 catgcgtctc agaagagtac ttttacctac accggaatta cccgacagac atatatacag
    14281 aggatttcga gttttattct tctctttttt atctgctgac caaggatcca tatacaagta
    14341 attatatctc atactgtaaa ctccattttg tccaaccaaa agcggttatt cttacatttt
    14401 tgcatgattt cattatttcc gaaaattgca ctgattatat gatcttgtga atctgtcagc
    14461 atatttccca acagagtata gttttctcca tcagggataa taatgtcaaa attgggttcc
    14521 ataacaacat tggctttgtc ccgttcttcg aaagttgccc aactaatggg catgttaggg
    14581 tgttgttttt ttagcattac cattttatct ttaaatatat ccgtattgat ttccagttta
    14641 tctctttttt cttttccgac tcctagagga cagaactggt aaaatcgcca agcttggatc
    14701 ttatagggca gagaatcaac ccagcaaaca atatcactga gatgattaat gttagactga
    14761 gatacgacgg tcgtgagttt tatattaatt cgattattat tttggggtaa ccatgttaat
    14821 atatttttga taaccctgtg ttggttactc attttatcac tgcgaatttc tgaattgaca
    14881 agactgtcaa ttccatcaat tggtattcct acccagtcta acaccggatt taaccgagca
    14941 tgatccataa taaatcgatg cccgaccgtg gagagatatg tcataaaacc cagttgctta
    15001 gcgtattcaa tcagatcaat gaaatcactc cgggatgtag gttcccctcc ggtaaaaccg
    15061 atacgacgga atcccatttt atacatccgc tcaattacat tacgagcact agcaagagaa
    15121 gagtcctgaa ttccaggtgt acgaaaacac agatcacatc ctaggttaca atgtgaagtt
    15181 attctgaaat ctaaaagaag ggtgctaata tccataacaa gtcctctgac tatgatacat
    15241 caataaccat taaaacataa tggttattgc agaccatgac aaagcgcaac cttcggctta
    15301 ccagggatat aatatctgcc agtaatcaga gcagtgggtt gcgcaaggaa ctcatgaatg
    15361 atgagttccg gtaatcgcat tactggaagc ccatgatcgg agggacgcta tcagtcacac
    15421 cagtgaaaat tgtgcacaga agcattttca tcactgacag gccgtatgcc tcacaagacg
    15481 ggagataact ggagaatatt tagttcttct accggcttac gccgatttta ctcctgtgtt
    15541 atatgtaacc attgtgtgcc accttccatg cgctggtgcg gcatcagagt aaaaaatcag
    15601 cattaaattt atttgcaaat gtatgagtaa tacagacttg ttaacgggtc ctttcagatg
    15661 tatctttaag acttattaaa cagtcttaaa aggtgcatta tgcctaacat cattctgtct
    15721 gacatcagtg caagtgtcag tgaactgaaa aaaaatccga tggcgacagt cggtgctggt
    15781 gatggttatc cggtcgccat tctgaatcgc aatcagccgg cattctactg tgttcctgct
    15841 gaactgtatg aaaggatgct tgatgctctg gatgaccagg agctggtgaa actggttact
    15901 gaacgtagca gtcaacctct gcatgatgtt gacctggaca gttattcatg acttatacga
    15961 taaaattcag ggatgatgcg cttaaagaat ggctgaaact ggataagacc attcaacaac
    16021 agtttgtgaa aaaactaaaa aaatgtagca aaaatcctca tatactgtct gcaaaactac
    16081 gtgggctaaa ggactgctac aaaattaaac ttcgtgcatc gggatttcgt ctggtttatc
    16141 aggtgattga tgatatgtta attattgcgg ttgttgcggt aggtaaacga gaacgtagta
    16201 acgtttatta tctcgccagt gagagaatga gatagtaaca taaatagcct ttcccgcaga
    16261 taactctcag aattgttatt ctctctgtgg cctcgcctga ggcggaaaaa gaaaagtttt
    16321 gttgagagaa tgattttacc tgccgggatg agtctggggt tatttatatt accagacaaa
    16381 ttgaaacgga gggacatgtt tgatgacaat acttgtgacg gcacaggctc aactcatagt
    16441 tggtgaggct atggcgataa aaagtttagc tcctgatgga atgttggccg ctgtatttga
    16501 agacgacggg aataccggat atttctatgc acttgatgag tccgttgaag gtaaccctgt
    16561 cagggatgct gtgcatatat ataacgttga agacatttca gatgctcaca tcccctctga
    16621 tgtgaagatt ggctggtctg aggattgcct gaagtgtgtt ctgctgataa acggatatcc
    16681 acatggggta ttcgattttg aggggaagaa cggctactgt cgaagtggat tcccgccacc
    16741 ggttaatcat gaatggtctg ttcaggggca ttcatgggat gattacgtcg ataatttctt
    16801 ccggtgattg aaccgaaaaa ttacctgacg gacaagtctg gggccgaatc gtgatgattc
    16861 atcatggccg tttatagttc agcagtcttg ttcttttgct gtcagtggga taccaggaaa
    16921 ataaccgatt gttaccagac agaataacca tcataagacg tcacaagacg gtgacgctta
    16981 catagttccg gggagttaaa caatataccc tgactttgta atcatttgaa ttatatccgt
    17041 aacgtgtaac ttcctgccgt aacaccggat ttaagatatt caaactttat ctttataccg
    17101 atattaagag acacaatgcg aaaaataatt gctcatttca agatagtttt aaccttactt
    17161 ctgcccgcaa cagtatctgc ccagtcgata gagtggcaat cctgtatgac cactccattc
    17221 agtaactggt ttggtgagga aaaaccgtct cctgacttac tatgtggtta tttgtctgtt
    17281 ccattaaaat atacagacac aggcggagat agttcttctg taaaaagaca gcaggtaaaa
    17341 ctggcgctga caaaattgcc agcaaaaagc aggcataaag ggagtatcat tataataagt
    17401 ggtggaccag gattacctgg cattaatcct tatattaact ttgactggcc ggtaacaaat
    17461 cttcgcgaat catgggatat tattggcttt gatccacggg gagtcggcca gtccattcct
    17521 gcaataaact gccagcaatc agatacaggg attcaggaaa acataactga aaagcaacgg
    17581 gtattaaata aaattaacag ctgtatccat aataccggaa ctgaagttat tcgccatatt
    17641 ggttctaacg aggcggtata tgatattgat cgtatcaggc aagccctggg tgacgagcaa
    17701 ctgacagctg tggcatattc gtatggaaca caaattgcag ccttatatgc agaacgtttc
    17761 ccatccagca tcagatctat cgttcttgat ggggtcgtcg atattgatga cctgggggac
    17821 aacttcacat ggcaactcaa acaagcacag agttatcagg aaacgttcga tcgctttgca
    17881 tcctggtgtg cacgtacaaa aagttgccca ctttcttcag tcagagataa ggcaataact
    17941 cagttccatg agctgttatc aaaattgcat cacaagcctt tattagacag caagggagaa
    18001 aatatatctt cagatgaact catattatta acaacagacc ttttgttatg gcgttcatca
    18061 tggccaaccc ttgcaactgc catacgtcag ttctctcagg ggattgtcag taatgaaatt
    18121 gaaactgcgc tcagtactcc tatagcctca gaagagtcaa gtgatgctct gggggtaatc
    18181 ctttgtgttg atcagggggg ggagcaatta accccagaag agcgaaaatc ccgaaaagat
    18241 gctcttgcga atgccttccc ggctattaac tttgacaatg gacgttccga ttcacctgat
    18301 ttttgtgaat tatggccaat acatagcaac ctgaataaaa cccggctgaa aaatacagtt
    18361 ctgccctctg gtttactgtt tgtagcacac aaatacgatc caacaacacc atggattaat
    18421 gctcgtaaga tggcagagaa attttccagc ccgttgctga caataaatgg tgatgggcat
    18481 acattagctc ttaccggagt caatttatgt gtagataagg ccgttgttcg tcatcttatc
    18541 acccctatga aaatgggaaa tatatcctgt ccaggaaatg ctgaaataga tataaaatag
    18601 tttaaaaatt gcccgccatt tgctcctgat accggacata ttttgcattg ggagcaaaat
    18661 gcgttcagtg cgatcacggc accagaaacg tcaaactgga tacacgtaaa aaatgtcgcg
    18721 atcgcgcaaa atcaagccat tactctcggg agtgcttaaa taatacgcac aatatacaac
    18781 gctctcttct ctacagtctg tttcctgtaa tatcctgcgg ttattttgat ttctcagaaa
    18841 tattttcctg ccgggccttc atactcagcc acctctgctc tttcgccgcc agccagcgcc
    18901 tggaattttg agcatcacat tccgatactt ccccctctgg ttgaccatcc aggttataac
    18961 ggggagttcc tgcaacagag gttctctgca ggtagatccg gcgactggta atgcaggata
    19021 ctgcacaatt ccattcgtcc agggtcagcg ctgtttccgg atgtgctgcc agatatgtag
    19081 taatgtcacg ggttatgcct gttttcagag gcagaaaacc gcccccttcg gcacgaagtt
    19141 gaggaaacag cgctttcacc ttctccagac aatcgtgagt gaactcactg gtccagtggg
    19201 atggtcttgg aaagcgttgt cttttctttt tcttcttcct ggctggcgct tcggggatat
    19261 gagtttcgac ggcaggtgaa ctgattccgg aggagactga ttctgtcgcc tgttcagtga
    19321 gtgcaggcgc gataacatct tttttttcgg ctaaggaagg cggtgaaaaa gagcgacgtt
    19381 ttttcaccac aatgacggga tttttctttt cttccagatc ttttcccata aatacctctg
    19441 cttcagaa
//
LOCUS       DAZGQL010000092        19270 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.092, whole genome shotgun sequence.
ACCESSION   DAZGQL010000092 DAZGQL010000000
VERSION     DAZGQL010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19270
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.092"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            35..568
                     /locus_tag="ACHKIR_003869"
     CDS             35..568
                     /locus_tag="ACHKIR_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO9991547.1"
                     /translation="MNAKIRYGLSAAVLALIAAGAPAPDILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGATRVDGKPVVPGMKLSKKKCDQVNAIERDKALAWVEKNIRVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWGIDR"
     gene            842..1537
                     /locus_tag="ACHKIR_003870"
     CDS             842..1537
                     /locus_tag="ACHKIR_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFO9991548.1"
                     /translation="MKEMISVDHEISMSSLDFLNNIINPIRVEAGKPPHEPRKFLAKI
                     EDELELEVTWKKFRLNNNQTRTAYYDLDFDQMMLVGMRESKAVRRSVLARLKAMHGIQ
                     IPRTLPEALRFAAKLAEQKAVLENQLAIAAPKAEFVDNYVEASGLMGFREVAKLLGIK
                     ETDFRLFLLENGIMYRLAGKMTPYSHHLDAGRFSVKTGEAGNGHAFTQVKFTPKGVQW
                     IAGLLAAWRATAA"
     gene            1632..1763
                     /locus_tag="ACHKIR_003871"
     CDS             1632..1763
                     /locus_tag="ACHKIR_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9991549.1"
                     /translation="MSDKLIMLAKGLCVIVGISFSLMLVVLFLSITWMALTSAGLVG"
     gene            1986..2192
                     /locus_tag="ACHKIR_003872"
     CDS             1986..2192
                     /locus_tag="ACHKIR_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HFO9991550.1"
                     /translation="MRELKMKLCVLMLPLVVSACGSTPPAPVPCVKPPAPPAWAMMPP
                     SNSLRLLDETFSVSETESSATRQH"
     gene            2257..2481
                     /locus_tag="ACHKIR_003873"
     CDS             2257..2481
                     /locus_tag="ACHKIR_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9991551.1"
                     /translation="MKKLLVTVKPFQGTIPFRILQRGRVLVEGSFSGKCTQLHSRTFQ
                     VNATNEELTVECTMNAAKCRMVSAALQPVC"
     gene            2826..3152
                     /locus_tag="ACHKIR_003874"
     CDS             2826..3152
                     /locus_tag="ACHKIR_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HFO9991552.1"
                     /translation="MKRNLPLIILLSSLVMGCTQHKTDMPRQLVKALPQYPAYAAANY
                     IKGRVDVKFDIGADGTVTRIEFIRSEPHHLFDEQVVKAMAKWRFEKDRPRKGVKKTFI
                     FSPSAP"
     gene            complement(3284..3484)
                     /locus_tag="ACHKIR_003875"
     CDS             complement(3284..3484)
                     /locus_tag="ACHKIR_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO9991553.1"
                     /translation="MSSKNRTRRTTTRNIRFPNHMIEQINIALEHKGSGNFSAWVIEA
                     CRRRLATDATHLRPASMTNNEK"
     gene            3526..3891
                     /locus_tag="ACHKIR_003876"
     CDS             3526..3891
                     /locus_tag="ACHKIR_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFO9991554.1"
                     /translation="MAELRTLQSRIKTLNTRRVNILKGEQRRVSGSARVSLKRHIWLR
                     DAGQCCLCGRVVDLCDSELDHRIALQFGGGNEETNLWTLCTECHRQKSAREAAGGMPD
                     PTLPEVSGGSGRADDIIGL"
     gene            4176..4733
                     /locus_tag="ACHKIR_003877"
     CDS             4176..4733
                     /locus_tag="ACHKIR_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFO9991555.1"
                     /translation="MLTTQKRKFALALMSGKNRTAAAIEAGYSEKSARCKGSQLAKDP
                     EVLAFIARKQGETVEVDEVPVYRQKKTQACHREVPPVEKKNQVSVPPDDGQSFSPLSG
                     IDYMEDGLPDPVKAMGRILVENISIDPKLALDAAWRLAQFTHHKKGDAGKKSAKGDAA
                     KKAANRFAVPPPPRLVVNNDNEGNG"
     gene            4730..6391
                     /locus_tag="ACHKIR_003878"
     CDS             4730..6391
                     /locus_tag="ACHKIR_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFO9991556.1"
                     /translation="MIPVWSTACPDWAERLKKGLSIIPAPIYPDQAAHALAIFKQLRI
                     VDAPGSPTFGESCAPWVFDLVAALFGSYDAQTGVRHIKEVFILIPKKNSKSTLAAGIM
                     MTALLLNWRQAAGYTILAPTVEVAANAFNPARDMVRRDDDLDDLCQVQTHIRTITHRV
                     TDTTLKVVAADPNTVSGIKSVGTLIDELWLFGKQYKAEDMLREAIGGLASRPEGFVVY
                     TTTQSNEPPAGVFRQKLQYARDVRDGKINDPHFLPVIFEHPPEMVERGEHLLMENLAM
                     VNPNLGYSVDEAFLYREYRKAREAGEEAFRGFMSKHANVEIGLALRSDRWAGADFWEQ
                     QGRRVSLDDILQRADVVTVGIDGGGLDDLLGMYVIGRDRETREWLGWGHAWAHETAVV
                     RRKSEASRFQDFVASGDMTIVRRVGDDTAEVAEYVRRIHEAELLDHIGIDPSGVGQIL
                     DSLAEAGIPDGIVVGISQGWKLGGAIKTTERKLAEGVLVHGGQPLMAWCVGNARVEPK
                     GNAILITKQASGRGKIDPLMALFNAVSLMSLNPEPKKKEYAVFFI"
     gene            6455..8392
                     /locus_tag="ACHKIR_003879"
     CDS             6455..8392
                     /locus_tag="ACHKIR_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFO9991557.1"
                     /translation="MTLKRACSLLTVKSFSEDERVITGIASTPSPDRDGDILEPEGAE
                     FGSAIPFLWQHDHSRPVGQCTVRRVSEGLEITATLVKPVPDMPSQLAARLDEVWAAIK
                     TGLVRGLSVGFRPHEYTFLDGGGVHFLRWELMEVSAVTVPANAECTIRTIKSYDRPFS
                     AASGNRKPVVKIASSAGAAAQSTTVFHKEKTIMNIGEQIKSFENKRAALAASLEEVMT
                     KAAEEGRTLDVEEEEHYDNTAAEIRQVDAHLKRLRELEAGKAATAQPVKQAGNGNVAA
                     VASAPVIRVEQKLDKGIGFARFAKSLAAAKGVRSEALEVARRQYPDDSRLHHVLKSAV
                     GAGTTTDPQWAGSLSEYQEYAQDFIDYLRPQTIIGRFGQGGIPALRQVPFNIRVHAQV
                     SGGAAGWVGEGKAKPLTKFDFESITFSHAKVSAIAVLTEELIRFSSPAADALVRNALA
                     EAVVARLDTDFVDPKKAAVADVSPASITHDVKGTASSGNPDADAEAAFGQFVAANLQP
                     TGAVWLMSSTNALALSMRKNALGQKEYPDMTLLGGSFQGLPVIVSQYVGDQLVLVNAP
                     DIYLADDGGVAVDMSREASLEMQSEPTGDSTTPSPVELVSMFQTGSVAIRAERWINWR
                     RRRTAAVAVITGVNYGSASGG"
     gene            8437..8658
                     /locus_tag="ACHKIR_003880"
     CDS             8437..8658
                     /locus_tag="ACHKIR_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9991558.1"
                     /translation="MAKIRYLQGTHDARAGDIRDVAQPCAEVLVRLGKAEYITVRRPA
                     GQKKKRDAEHGECGTFYGEPEKTRNQDVT"
     gene            8604..11026
                     /locus_tag="ACHKIR_003881"
                     /pseudo
     CDS             8604..11026
                     /locus_tag="ACHKIR_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365916.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            11023..11349
                     /locus_tag="ACHKIR_003882"
     CDS             11023..11349
                     /locus_tag="ACHKIR_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFO9991559.1"
                     /translation="MTALLTLEEIKAHLRVDHDADDDMLMDKVRQATAVLLAYIQGSR
                     DKVIREDGELIPGEALTRMKGAAMRLTGMLYRNPDLAEREELLQGELPFSVSVLIYDL
                     RCPTVL"
     gene            11359..11709
                     /locus_tag="ACHKIR_003883"
     CDS             11359..11709
                     /locus_tag="ACHKIR_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFO9991560.1"
                     /translation="MAISAGRLTQMISVLNPVLTRNAAGEMTEEWVSCGKIHADIRGR
                     SSRERMQSGAEMAQAEIRIWVRGQSGREITAASRLHVLSGPWRDRILNVVGLPVPDAT
                     GGRLEILCRLGGEK"
     gene            11706..12152
                     /locus_tag="ACHKIR_003884"
     CDS             11706..12152
                     /locus_tag="ACHKIR_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFO9991561.1"
                     /translation="MIETLLDFSGLEEISRDLQLLSGAENNRVLREATRAGANVLKEE
                     VASRAPVRRGKLRRNVVVLSRRSRDGGMESGVHIRGVNPDTGNSDNTMKADNPRNAFY
                     WRFVEMGTVNMPPHPFVRPAFDVRSEQAAQVAIARMNRAIDEVLRR"
     gene            12149..12493
                     /locus_tag="ACHKIR_003885"
     CDS             12149..12493
                     /locus_tag="ACHKIR_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HFO9991562.1"
                     /translation="MTEADLYPHLAHLAGGQVYPYVVPLLDGRPSVALPWVVFSLISS
                     VSADVMGGQAESSVAVQIDVYAGTVTQARQIRQDAREAIMLLAPGSVSEMQDYIPENR
                     CYRATLEFQVTV"
     gene            12559..13275
                     /locus_tag="ACHKIR_003886"
     CDS             12559..13275
                     /locus_tag="ACHKIR_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major tail subunit"
                     /protein_id="HFO9991563.1"
                     /translation="MSALYERSQLTQVMISSAPATAETMDKAEYLRLDCTIKEVQFTA
                     GQKQDIDVTTLCSTEQENINGLGASSEISMSGNFYLNQAQNALRDAYDNDALYAFKVQ
                     FPSGKGFKFLAEVRQHTWSSGTNGVVAATFSLRLKGKPVSFVVPLAFVKNLDKTLTVN
                     TGALLTMSVSANGGTPPYKYAWKKDGQPVDGQTTDTFSKPGAQSADAGKYTCVVTDSA
                     EKAQSVTSVECTVTVSAAAG"
     gene            13290..13664
                     /locus_tag="ACHKIR_003887"
     CDS             13290..13664
                     /locus_tag="ACHKIR_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HFO9991564.1"
                     /translation="MKKDLKTLALARLSGFRHKTVKVPEWGNVSVVLREPSAEAWYLW
                     QDVLNGDGEDDDTLSVVAKTRRNLEADVTLFCDVLCDTDLQRVFTPDDREQVLAVYGP
                     VHARLLRQALELIADAESARKK"
     gene            13760..13969
                     /locus_tag="ACHKIR_003888"
     CDS             13760..13969
                     /locus_tag="ACHKIR_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HFO9991565.1"
                     /translation="MWMEFDRVSPLGDERGDIRNAQIVKAVFGAQGMNVALKDAMLCW
                     GEDEDKPEADPLAALEDALLFASEN"
     gene            14033..14446
                     /locus_tag="ACHKIR_003889"
     CDS             14033..14446
                     /locus_tag="ACHKIR_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9991566.1"
                     /translation="MKKFMLLIFSVSFFLTGCATIVGDKTQTVQVNSNPSGADFIIKD
                     ESGKTISSGKTPQNVTLEKSDGSYFGKKSYQITFSKENYQPVTLPIKASANGWYIGGN
                     FVFGGIVGWLLVDPFNGGMYTLSPESLNPGLVPKT"
     gene            14510..17752
                     /locus_tag="ACHKIR_003890"
     CDS             14510..17752
                     /locus_tag="ACHKIR_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO9991567.1"
                     /translation="MATLRELIIKISANSQSFQSEIQRASRMGSEYYRTLQNGGRQAA
                     AAAREQRRALAELNSQLTEIRASAVGMTGAFAGAFATGHLISLADEWSSVNARLKQAS
                     QSSDEFSSSQKVLMDISQRTGTAFSDNAALFARSAASMREYGYSADDVLKVTEAISTG
                     LKISGASAAEAGSVITQFSQALAQGVLRGEEFNSVNESGDRIIRALAAGMGVARKDLK
                     AMADDGQLTADKVVPALISQLGILRDEYAAMPETVSDGITKVENAFMAWVGGANEASG
                     ATKTLSGALNGVAGNIDTVATAAGVLVAVGVARYFGNLASGAMSATAGLVTAARNEVA
                     LAEAQLRGTQIATARARAAVYRAQQAVAAARGTEMQIAAEARLAITQERLNRNIAART
                     AAQNALNSTTAVGSRLMSGALGLVGGVPGLVMLGAAAWYTLYQNQEQARESARQYALT
                     IDEIAHKTPSMSLPEASDNEERTREALTEQNRLIDEQASRVKSLQEKIAGYQYVLANP
                     GWTTGNGFMINHLTSVKTVTEELAQATEQFAVEQSRLAQMQEKAQSIQDVLAGLEERR
                     VVLIRQQAAEQNKAYQSLLVMNGQHTEFNRLLGLGNELLQQRQGLVNVPLRLPQATLD
                     DKQQSALNNTERQLALSRLKGEEKERARLGYAADDLGLVGDTYQEARQRYIRNSMEAW
                     RNNEANKPKSRAGKSETEKAEDSFSRLLKQQKEQLALAGKNTELAKLKYQTSQGELKT
                     LTEIQKQELLRNAALIDQKKIREQLRAREEALKNDNADERASNDAELLGYGQGERVRE
                     RMRELQQIRDGYRQKDADLQSQYQTGDISEDFYRQALAQNAQYLSERLKDQEAFYAES
                     DAQRADWQKGLQEGLSNWVDSASDYASQAAQLATDGISGMVNNITEMLNGNKVEWRSW
                     AASILQEISKVLMNAAIVNGIKMAANSMSGAGGFFGSIGNWLGGAVANAKGGVYTSAN
                     LSAYSNSIVDTPTYFAFAKGAGLMGEAGPEAIMPLTRAADGSLGVRAVGSMNGSAGLV
                     YSPVYHIAIQNDGANGQIGPEAAGTLVQLIDQRVQAVMLSMRRDGGMLSG"
     gene            17745..18086
                     /locus_tag="ACHKIR_003891"
     CDS             17745..18086
                     /locus_tag="ACHKIR_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9991568.1"
                     /translation="MDEIKTLHWCPREGMQVTEKPSVVTVKFGDGYQQRRPAGLNAQL
                     KTFQVVFRVTTDAEREALSAFLSWHGGYRAFLWKPPKHNRTVRVVCREWSITDNARYS
                     DFSCTIEQVVN"
     gene            18086..18784
                     /locus_tag="ACHKIR_003892"
     CDS             18086..18784
                     /locus_tag="ACHKIR_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO9991569.1"
                     /translation="MQDIHEESLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEPVTWQGREYQAYPIEGSGFEMNGKGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELTDRWVVEQMSSLTAMTASFVLATPTETDG
                     ALFPGRIMLANTCMWDYRGDECGYNGPAVADEFDNPTTDIRKDRCSKCMRGCELRRNV
                     GNFGGFLSINKLSQ"
     gene            18795..>19270
                     /locus_tag="ACHKIR_003893"
     CDS             18795..>19270
                     /locus_tag="ACHKIR_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO9991570.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVISTPEGERYQPCVNISAEPE
                     AYFRITPEDWLQAQMQGEIVALVHSHPGGLPWLSEADRRLQIKSALPWWLVCRGDIHR
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFVREDDWWRNGQNLYLDNLA"
BASE COUNT         4409 a         4534 c         6095 g         4232 t
ORIGIN      
        1 ggtattcgga accgataaat cctgataaat atccatgaac gcaaaaatca gatacggcct
       61 gtcggctgcc gttctggcgc tgattgccgc aggggcgcct gcgcctgata ttctcgacca
      121 gtttctggat gaaaaagaag gtaaccacac cacggcatac cgtgatggtg cgggtatctg
      181 gaccatttgt cgtggtgcca ccagggtgga tggcaaacct gtcgtcccgg gcatgaagtt
      241 gtcgaagaaa aaatgcgacc aggttaacgc cattgagcgt gataaggcgc tggcatgggt
      301 ggagaaaaac atccgggtgc cactgaccga accccagaaa gcggggatcg cgtcattctg
      361 cccgtataac attggtcccg gtaagtgttt cccgtcgacg ttttataaac gaattaatgc
      421 aggcgatcgc agaggagcgt gtgaagcgat tcgctggtgg attaaggacg gtggcagaga
      481 ctgccgtatc cgctcaaata attgctacgg tcaggtatct cgtcgtgacc aggagagtgc
      541 gctggcgtgc tggggtatcg acagataagc agaatatttt gctgaaaaat gacgttggcc
      601 aacgcggacg gataacacga aatcctgcga actggcaaaa tgtaagtgaa taaagtcagg
      661 atcattattt cacgcagagg caccgtaatg gtgcctttgt catttctgcg cttcgcacaa
      721 gcgtaaataa accaaagaac ctttcaggat gagccctggt ggataaccgg cagtggtctg
      781 gttaaccctc tttgggctgg ttattcctgt gcgcagggtt catcactaaa aggaatcaac
      841 catgaaagag atgatttctg tcgatcatga aatatccatg agtagtctgg attttctgaa
      901 taacattatt aaccccatcc gggttgaggc agggaaacca cctcatgaac cgcgtaagtt
      961 ccttgcaaaa attgaggacg aacttgagct tgaggtaacc tggaaaaaat tccggttaaa
     1021 caataaccag acaagaacgg catactatga tctggatttt gaccagatga tgctcgttgg
     1081 catgagggag tcaaaggccg ttcgtcgttc tgtgctggca aggctcaaag cgatgcatgg
     1141 tattcagatc ccacggactt tacctgaggc gttgcgattt gcggcaaaac tggctgaaca
     1201 gaaagcagtg ctggaaaatc aactggcaat agcagcgccg aaggctgaat ttgttgataa
     1261 ctatgttgaa gcatctggtc tgatgggatt ccgggaagtt gctaagttac tcggtatcaa
     1321 agaaaccgat ttccggctgt ttttgttgga gaacggaata atgtatcgcc tcgctggaaa
     1381 aatgacgcct tactcgcatc acctggatgc ggggcggttt agcgtgaaaa cgggcgaggc
     1441 gggcaatggt catgctttta cgcaggttaa attcacccca aaaggtgttc agtggattgc
     1501 tggtctgctt gctgcatgga gagctaccgc agcatgaaga tgataaaaat ggactggaaa
     1561 tttttgctgg tctggctgat tccgttttta tgggtggttg cccggttaat tactgctatt
     1621 aaggggtaaa gatgtcagac aaactcataa tgctggcgaa gggcctttgt gtaatcgtcg
     1681 gtatttcatt ttcactaatg ctggttgttc tttttctttc cataacctgg atggcgttga
     1741 cttcggcagg gctggtgggg tgagcataaa ccgaatgctt tccgcgttta ccgttattct
     1801 gcttgtggtc tgtggtgcgc ttagtctggg gctgaatcat taccgcgata atgccatcac
     1861 ctacaaagcg cagcgcgata aaaaagccag agagctggag caggcaaacg caaccattac
     1921 tgacatgcag cagcgccagc gcgatgttgc tgcgctcgat gcaaaatact cgagggaatt
     1981 agccgatgcg agagctgaaa atgaaactct gcgtgctgat gttgccgctg gtcgtaagcg
     2041 cctgcggatc aacgccacct gccccggtac cgtgcgtgaa gccaccggca cctccggcgt
     2101 gggcaatgat gccgccgtcg aactctctcc ggttgctgga cgaaacgttc tcggtatcag
     2161 agacggaatc atcagcgacc aggcagcatt gagaatgctt caggaatata tccgcactca
     2221 gtgtattaac tagtattttt gttattcgga gaatgcatga agaaattact ggtaaccgta
     2281 aagccttttc agggaacaat tccgttccgt attttgcagc gtggtcgtgt tcttgttgaa
     2341 ggttcgttca gtggtaaatg tacgcaatta cactcccgga cctttcaggt gaatgccacg
     2401 aatgaagagc taaccgttga gtgtacgatg aatgccgcta aatgccgtat ggtatcggct
     2461 gcattacagc cagtgtgttg agcgacctta ttatccatgc gcggtattgt cgccgtattc
     2521 ccgtattaac agagaccgca gcccgacagg gagactcctc tgcgcgagtg tgcggggata
     2581 atcaaaaacg atacacaccg gggtttaccg cgttaacgga gcgcggcgtt gtcctctcat
     2641 ggtcgctggt ccggtgcgat ggtggaagaa accggacgat gtgttacctc acaagacctg
     2701 ttatgtcatg tgtctgattt gtgatttaag tcggataatt gtcgttgcca ttaagcagag
     2761 gattgatggc cgacagggtg gcattgttag aataagactt attcttatct gtgccgggaa
     2821 tgaaaatgaa aagaaatctt ccgttaatta ttttgttgtc ttctctggtt atgggctgta
     2881 cgcaacataa aacagatatg ccccgacagt tggttaaggc attaccacaa tatccggcct
     2941 atgcagcggc aaattatata aagggacggg ttgatgtgaa gtttgatatt ggtgctgatg
     3001 gtactgtcac ccgaattgag tttatccgtt cagagccgca ccatctgttt gatgagcagg
     3061 ttgtaaaggc gatggcaaaa tggcgatttg agaaggacag gccgcgtaaa ggcgtgaaga
     3121 aaacgtttat ctttagtcct tctgcaccct gattatttca tcagaaatta attatcactc
     3181 tgttgttatt ctgtacatcc cggctgggta agtcttgttc cgccgggtat gaagatgaaa
     3241 tattgttgga ggacagtggg tacctgctcc tgtaaccgaa cgttcatttc tcgttatttg
     3301 tcatgctggc cggacgcaga tgcgttgcat ctgttgccag ccttctcctg caggcttcaa
     3361 taacccacgc tgaaaagtta ccggaccctt tatgctcaag ggcgatgttg atctgttcaa
     3421 tcatgtgatt ggggaaacgg atattgcggg ttgtggttct gcgggtccgg tttttcgatg
     3481 acatatttat ttcctttact gattgccata tgacggggat tttacatggc tgagcttcgt
     3541 acactccaga gcagaatcaa aacactgaat acccgacggg tgaatattct gaagggtgaa
     3601 cagcgtcgtg tcagtggcag tgcacgtgtt tccctcaagc gtcatatctg gctcagggat
     3661 gccgggcagt gctgtctctg tggtcgtgtg gttgacctct gtgacagtga actcgatcac
     3721 cgaattgcac ttcagttcgg tggtggtaat gaggagacga atctctggac gctctgtacc
     3781 gaatgccatc gacaaaagtc tgctcgtgaa gcggcgggtg gtatgccgga cccgacgctg
     3841 ccggaggtgt ccggaggtag tggcagagcg gacgacatca tcggactgta acccgaccgg
     3901 gggggggtat cattcggcgt aaaaaacgat cgctccggac accgcgcccc ctctcacgca
     3961 gagaaaaaat tccccttcag aagttataaa catgtttata acctactgat ggatttttac
     4021 cgtttttttc tttttattca taacgttgtg ggaggtaaat gtttatacct ttttttcgca
     4081 aaatgtttac agggcagcgg taaacatttt ccctgccgcc tccattctta ccgggaggat
     4141 tgctgacgtt tttctgtttt tccccggagg aaagaatgct gacgacgcaa aaaagaaaat
     4201 ttgcgctggc gctcatgtcg ggcaaaaaca gaacggctgc tgctattgag gctggttact
     4261 cggaaaagtc cgccagatgt aaaggctcac aactggcaaa agatccggaa gtgctcgcgt
     4321 ttatagcccg taagcagggc gagacggtgg aggtggatga ggttcctgtt taccggcaga
     4381 aaaagacgca ggcgtgccac cgggaagttc ctccggtgga aaagaaaaac caggtttcag
     4441 tgccaccgga tgacgggcaa agtttctctc cactatccgg tattgattac atggaggacg
     4501 gccttcctga tcctgtgaaa gccatgggtc ggatcctggt ggaaaacatc agcattgatc
     4561 cgaaactggc tctggatgca gcctggcgac tggcgcagtt cacgcaccac aaaaaagggg
     4621 atgccggtaa aaaatcggca aaaggtgacg cggcgaaaaa agcggctaac cgttttgcgg
     4681 tgccaccacc accccgcctg gtggtgaata atgataatga gggcaacgga tgatacctgt
     4741 gtggagcacg gcctgcccgg actgggcaga gcgtctgaaa aaggggctgt cgattattcc
     4801 ggctccgatt tacccggacc aggccgcaca tgccctggcg atttttaaac aactgcggat
     4861 tgtggatgca ccgggcagcc cgacgttcgg ggagtcctgt gcaccgtggg tgtttgatct
     4921 ggtggctgcc ctgtttggct cctacgatgc gcagaccggt gtacgccata taaaggaagt
     4981 gttcattctg atacccaaaa aaaacagcaa gtccacgctg gctgccggga tcatgatgac
     5041 ggcgctgtta ctgaactggc ggcaggcggc gggctacacc attctggccc cgaccgtgga
     5101 ggtggcggct aacgccttca accctgccag ggatatggta cgacgggacg atgatctgga
     5161 tgacctctgt caggtgcaga cacatatccg gaccatcacc catcgggtga cggacaccac
     5221 ccttaaggtg gtggctgccg atccgaatac ggtgtccggt atcaagtccg tggggacgct
     5281 gattgatgaa ctgtggttat ttggcaagca gtacaaagcg gaggacatgt tacgtgaagc
     5341 catcggcggc cttgcctccc gcccggaagg gtttgtggtg tacacaacca cccagtcgaa
     5401 tgagccgccc gccggggtgt tcagacagaa actgcagtac gcccgggatg tgcgcgacgg
     5461 caaaattaat gatccgcact ttctgccggt gatatttgag catccgccgg agatggtgga
     5521 gcgcggtgag cacctgctga tggaaaacct cgccatggtt aacccgaatc tcggttattc
     5581 ggtggatgag gcttttctgt accgggagta ccgtaaagcc cgggaggctg gtgaggaggc
     5641 atttcgtggc ttcatgtcaa aacacgccaa tgtggaaatt ggtcttgccc tgcgttctga
     5701 ccgctgggcg ggtgcggatt tctgggagca gcagggcagg cgcgtcagcc tggacgatat
     5761 cctgcagcgc gctgatgtgg tgacggtggg gattgacggc gggggcctgg atgatctgct
     5821 gggaatgtac gtgattggtc gtgacaggga aacccgcgaa tggctgggct ggggccatgc
     5881 ctgggcgcat gaaaccgcgg tggtcagacg gaagagtgag gcatcccggt ttcaggattt
     5941 tgtggccagt ggagacatga cgattgtccg tcgggtcggg gatgacacgg cggaagtggc
     6001 ggagtatgtg cgtcgtattc atgaggctga gttactggac catatcggta ttgacccgtc
     6061 aggtgtgggg cagattctgg attcactggc agaagccggg atccccgacg gaattgttgt
     6121 ggggataagc cagggctgga agctgggcgg ggccatcaaa accaccgagc gcaaactggc
     6181 tgagggagtg ctggtgcatg gtggtcagcc actgatggcc tggtgcgttg gcaatgcccg
     6241 ggtggagcct aaaggtaacg ccatccttat taccaaacag gccagcggac gggggaaaat
     6301 tgacccgctg atggcgctgt tcaatgcggt atccctgatg tccctgaatc cggagcccaa
     6361 aaagaaagaa tatgcggttt ttttcatata accctgttca ccctgtaacc atcatggacc
     6421 gctgcggcgg tttttttatt ttcaggaggc tgatgtgact cttaaacggg cctgctccct
     6481 gctgacggtg aaatccttca gtgaggatga gcgggtgatc accgggattg cgtcaacgcc
     6541 ttctccggat cgggatggtg acatcctgga gccggagggc gcggagtttg gcagtgcgat
     6601 cccgtttctc tggcagcatg accattcccg cccggtgggg cagtgtacgg tgcgccgggt
     6661 cagcgaaggg ctggaaatca cggcaacact ggtgaaaccc gtaccggata tgccgtcgca
     6721 actggctgcc cggctggatg aggtctgggc ggccattaag accgggctgg tcagggggct
     6781 gtccgtgggc ttccgtcctc atgaatacac ctttctggac ggaggcggag tgcattttct
     6841 gcgctgggag ctgatggagg tgtctgccgt caccgtgccc gcgaatgcgg aatgcactat
     6901 ccggaccatt aaatcttacg accgcccgtt ttctgccgcg tccggcaacc ggaaaccggt
     6961 ggtgaaaatc gcatcttctg ccggcgctgc ggcacagtca acaaccgttt ttcataagga
     7021 aaagaccata atgaatattg gcgaacagat taaaagtttt gaaaacaagc gtgcagcgct
     7081 ggcagcctcc cttgaggagg tcatgaccaa agccgcagag gaagggcgca cgctggatgt
     7141 ggaggaggaa gagcattacg acaacaccgc agcggaaatc cgtcaggtgg atgcgcacct
     7201 gaagcgcctg cgtgaactgg aagccggtaa ggccgccacg gcgcagccgg tgaaacaggc
     7261 cggtaacggg aatgtggccg cggtggcttc tgcgccggtg atccgtgtgg agcagaaact
     7321 ggataagggg attggtttcg cacgttttgc caaatcgctg gctgcggcta aaggtgtccg
     7381 ctctgaagcc ctggaagtgg cccgtcgtca gtatccggat gacagtcgtc tgcatcatgt
     7441 cctgaaatcg gcagtgggcg cggggaccac cacggatccg cagtgggcag gcagcctgtc
     7501 tgaatatcag gaatacgcac aggactttat tgattacctg cgtcctcaga ccattatcgg
     7561 gcgatttggt cagggcggga tccctgcact tcgtcaggtg ccgttcaata tccgtgtgca
     7621 cgcccaggtg tccggcggtg ctgccggctg ggtgggtgag ggtaaggcaa aacccctgac
     7681 gaagtttgat tttgaatcca tcaccttcag tcatgcgaag gtgtcggcca ttgcggtact
     7741 gacggaagaa ttgatccgtt tttccagtcc ggctgctgat gcactggtcc gtaatgcgct
     7801 ggcggaagcg gtggtggcgc gtctggatac agactttgtg gacccgaaaa aagcggcggt
     7861 ggcagatgtc tccccggcgt ccatcaccca tgatgtgaag ggcacggcat caagcggtaa
     7921 cccggatgcg gatgcagagg ctgcgtttgg acagtttgtg gcagcaaacc tgcaacccac
     7981 cggtgcggtc tggctgatgt ccagcaccaa tgccctggca ctgtccatgc gtaaaaatgc
     8041 gctgggtcag aaagaatacc cggacatgac cctgctgggt ggctccttcc aggggctgcc
     8101 ggtgattgtc tcccagtacg tgggtgacca gctggtgctg gtgaatgccc cggatattta
     8161 tctggcggat gacggcggcg tggcagtgga tatgtcccgc gaggcatcac tggaaatgca
     8221 gtctgagccg accggcgaca gtaccacgcc gtcgccggtg gagctggttt ccatgttcca
     8281 gacaggcagt gtggccatcc gtgcggagcg ctggatcaac tggcgtcgtc gccgtaccgc
     8341 ggcggtggcg gtgatcaccg gtgtgaacta cggcagtgcg tccggcggct gagtctgata
     8401 aggaggacgg gaggcgtgcg cctcccgtaa caggttatgg caaagatccg atacctgcag
     8461 ggcacgcatg atgcccgggc cggggatatc cgggatgtgg cacagccgtg tgcggaggtg
     8521 ctggttcgcc tgggaaaggc agagtacatc acggtgcgac gtccggcagg tcagaaaaag
     8581 aaacgtgatg cggagcatgg cgaatgtgga accttttacg gcgaacccga aaaaaccaga
     8641 aatcaggacg tgacgtaaga gaggcgggct ggaccagcct gtttcaggcg gtggctgagc
     8701 ccttttccgg cgcctggcag cagggcgtga aagccgatcc tgaagccgtc ctctcctttc
     8761 atgcggtgtt tgcatgtatt tcgctgatat cccaggatat cgccaaaatg cggctgcgtc
     8821 ttatgcagac ggatgcgcag gggatacgca gggaaacgcg ccggggagat attgcccgcc
     8881 tctgtcgtcg tcccaacgcc cagcagaacc gcatccagtt ttttgaactg tggctgaacg
     8941 ccaaactgcg tcacggcaat acggtggtgc tgaaaatccg taatgcccgg gggcagatca
     9001 aagaactgcg tattctggac tggaaccggg ttgaacctct ggtggcggat gacggcgagg
     9061 tgttctaccg catcacgccg gaccggaact gtgggatcac tgaggcggtg acggtgcctg
     9121 cccgggaagt gatccacgat cggtttaact gtttttttca tccgcttata gggttgccgc
     9181 cggtgtatgc cgccgggctg gcggccacgc aggggcatca tattcaggaa aattcgacgt
     9241 cttttttcag aaatggcggc aggccgtccg gggtgattga gatccccggc agtattacgg
     9301 aagaaaatgc gaaaaaactg aagagcaact gggacagcgg gtatacaggc gaaaatgcgg
     9361 ggaaaacggc cattctgagt aacggggcaa aatacaaccc cacgacgttt tcaccggtgg
     9421 atgcgcagac ggtggaacaa ctgaagatga ccgctgaaat tgtctgttcg gtgttccgtg
     9481 tcccggccta caagattggc gtgggacaac cgccttccag tgacaacgtg gaggcgctgg
     9541 agcagcagta ttattcccag tgcctgcaga cactgattga gtccattgag ctgttactgg
     9601 atgaggcgct ggaaacgggg gaaaacgaga gtacggaatt tgatgtcacc acgctgctga
     9661 gaatggacag tgagcggcgc atgaaaacgc tgggggatgc ggtgaaaaat acgcttctca
     9721 cgcccaatga agcccgtaaa cgggagaacc tgccgcccct ggcaggaggc gatgcactgt
     9781 atcttcagca gcagaactac agtctggaag cgttgtcccg ccgtgatgcc cgtgaagatc
     9841 cgttcgcgtc gtccggtaaa acagcttccg tgcctcaggc ggtcgctgca tctgacggta
     9901 ataaggcaat cactgaaaca gagcatgacg cggtgaaggc gatgttcagg gggattctga
     9961 aaaaatgaat gaacgtgaac tgtccattat ccgtgcgctg ggtgaagaat tttccgcggt
    10021 gctggcggat ttacagcgca catttgagga gaaaatagcc gcgcaggcac aaacgtttga
    10081 agaaaaactg gcttccctgt ctgtggtatt acagaagtgc gtgacgggcg atgatgtgcg
    10141 tccgatgctt gagcagatgg tgaaggaggc ggtaagccat atccctgttc cgcgagatgg
    10201 tcgtgactac gatccggaag tactgcagaa ggcggtgaat gatgcggtcg caaatattcc
    10261 gcagccggcg gacggtaaaa gtatcacccc ggatgatgtg cgtccgatgc ttgagcagat
    10321 ggtgaaggag gcggtaagcc atattcctgt tccgcgtgat ggtcgtgact atgatccgga
    10381 agtactgcag aaggcggtga atgatgcggt cgcaaatatt ccgcagccgg cagacggtaa
    10441 aagtatcacc ccggatgatg tgcgtccgat gcttgagcag atggtgaagg aggcggtaag
    10501 ccatattcct gttccgcgtg atggtcgtga ctatgatccg gatgttctgc agaaggcggt
    10561 gaatgatgcg gtgagtgccc tgccggctcc gcaggacggg cgtgatgcca cggcactgga
    10621 aatactccct gccattgacg atcaaaaatc ctttccccgg ggcacgtatg ccacacacca
    10681 gggcggactc tggcgggcgt atgaaaaaac gcacgggatg cggggatggg aatgcctggt
    10741 tgacggggtg gcggatattg acgtcagcat gacgggtgaa cggttgttct ctgtggtggt
    10801 ccggcagagc agtggccagc gtacggaaaa aacattttcc ctgccggtga tgctctaccg
    10861 cggtgtgttc agagccggtg aaacctacca ccccggcgat acggtgacgt gggggggctc
    10921 gctgtggcac tgcaacagta tgaccggtga taaacccgga gaagctcatt catcaggctg
    10981 gaccctggct gcaaaacgtg ggcgggatgc tggaggcgga aaatgacggc attactgacg
    11041 ctggaagaga tcaaggcaca tctgcgtgtc gaccatgacg cggatgatga catgctgatg
    11101 gacaaggttc gtcaggctac cgccgtgctg ctggcctaca ttcagggcag ccgggataag
    11161 gtgattcgtg aggacggtga actgatcccg ggcgaggcat taacccggat gaagggggct
    11221 gccatgcgac tgaccgggat gctgtaccgg aatccggatc ttgcggagcg ggaagaactg
    11281 cttcaggggg agctgccgtt ttctgtttcc gtgctgattt acgatttgcg ttgtccgacg
    11341 gtgttatgag gagggggaat ggcaatatct gcaggtcgtc tgacacagat gataagtgtt
    11401 ctgaacccgg tgttaacccg taatgctgcc ggagaaatga cggaagaatg ggtgtcatgc
    11461 gggaaaattc atgcggatat ccgtggcagg agcagccggg agcggatgca gtccggtgcg
    11521 gaaatggcgc aggcggaaat ccgcatctgg gtgcgcggtc agtccggtcg ggaaatcacg
    11581 gcagcgtcac gacttcatgt gctgagtggt ccatggcgtg accggatcct gaacgttgtc
    11641 gggctgcccg tgccggatgc gaccggcggg cgtctggaaa ttctctgtcg gctgggaggg
    11701 gaaaaatgat cgaaaccctg ctggattttt cggggctgga ggagatcagc cgcgatttgc
    11761 agcttctgag tggtgcggaa aataaccggg tgctgcgtga ggcaacccgt gcgggtgcga
    11821 atgtgctgaa agaagaagtg gcgtcacggg caccggtacg caggggaaaa ctgcgccgca
    11881 atgtggtggt cctttcccgg cgctcccgcg atggcgggat ggaatccggt gtccatatcc
    11941 gtggtgttaa tccggacacc ggtaacagcg ataacaccat gaaggcggat aacccgcgca
    12001 atgctttcta ctggcggttt gtggaaatgg ggaccgtgaa tatgccaccg cacccgtttg
    12061 tgcgcccggc gtttgatgtg cgcagtgaac aggcagcgca ggtggcgatt gctcggatga
    12121 accgggccat tgatgaggta ctgagacgat gacggaggcg gatttgtatc ctcatctggc
    12181 gcatcttgcc ggcgggcagg tgtacccgta tgtggtcccc ctgctggatg gcaggccgtc
    12241 ggtggcgctt ccgtgggtgg ttttcagcct gatttcatcg gtgtctgcgg acgtgatggg
    12301 cgggcaggcg gagtcctcag tggcggtgca gatagacgtt tatgccggga ctgtgacgca
    12361 ggcgcgtcag atacgtcagg acgcccgtga agccataatg ctgctggccc cgggatccgt
    12421 cagtgaaatg caggactata ttccggaaaa ccgctgttac cgtgcaaccc tggagtttca
    12481 ggtcacggtg tgactttttc ttttttctac aaaacccata ccccgccgcg tgcgggtttt
    12541 ttattatcag gaggcagaat gtctgctttg tatgaacgct cacagctgac gcaggtgatg
    12601 atttcatctg ccccggcgac tgctgaaact atggataagg cggaatatct gcgcctggac
    12661 tgcaccatca aggaagtcca gttcaccgcc ggtcagaaac aggatattga tgtgaccacg
    12721 ctctgctcca cagagcagga gaatatcaat ggtctggggg cgtcgtccga gatttccatg
    12781 tcgggtaatt tttatctgaa tcaggcccag aacgccctgc gtgatgctta tgacaatgac
    12841 gcgttgtatg cgtttaaggt gcagtttccg tccggtaagg gctttaaatt cctggcggaa
    12901 gtgcgccagc acacctggtc atccggtacc aacggcgtgg tggctgcaac gttctcactg
    12961 cgtctgaaag gcaaaccggt gtcctttgtg gtaccgctgg cgtttgtgaa aaatctggat
    13021 aagacactta ccgtgaatac cggtgcgctg ctgacaatgt cagtcagtgc caacggggga
    13081 acgccgccgt ataaatacgc ctggaagaag gatggtcagc cggttgacgg gcagacgaca
    13141 gacaccttca gtaagccagg tgcgcagtcc gctgatgcgg ggaaatatac ctgcgtggtg
    13201 actgattcgg cagagaaagc acagagtgtg acgtctgttg aatgtacagt gacagtgagc
    13261 gcagccgccg gataagggga tgggtcatca tgaaaaagga tctgaaaacg ctggcgctgg
    13321 ccagactgtc agggtttcgt cataaaacgg tgaaggtgcc ggaatggggt aatgtcagcg
    13381 tggtgctgcg ggagccttcg gcagaggcct ggtatctgtg gcaggacgtg ctcaatggtg
    13441 atggagagga tgacgatacc ctgtcggtgg tggcgaaaac ccgccgtaac ctggaagcgg
    13501 atgtgacgct gttctgcgat gtcctgtgtg atacggacct gcagcgggtg ttcactccgg
    13561 acgaccgtga gcaggtgctg gccgtctatg gtccggtaca tgcccggttg ctgcgtcagg
    13621 cactggaact gatcgctgat gcagagtcgg ccagaaaaaa gtagcccgcc cggaaattcg
    13681 ctttctgatg cgacttgcgc tccgtctggg gcgcacctta tccgaactgc gccacagcct
    13741 gagtgcgagc gaggcgatga tgtggatgga gttcgacagg gtatccccgc tgggtgatga
    13801 gcgcggggat atccgtaatg cacagatcgt gaaagcggtt ttcggggcac aggggatgaa
    13861 tgttgcactg aaggacgcca tgctctgctg gggcgaggat gaggataagc cggaggcaga
    13921 tcctttggcc gctctggagg atgctctgtt atttgcttcg gaaaattaat tttaactttg
    13981 cagatcttta ttacagtatt ttcggggaaa attaactgaa cggagccatt ttatgaagaa
    14041 attcatgctg ttgattttct ctgtcagctt ttttctgacg gggtgtgcaa ccattgtcgg
    14101 agataaaacg cagactgtac aggtaaacag taatccgtct ggtgcggatt ttattattaa
    14161 agatgagtca gggaaaacaa tttcttcggg caaaacaccg cagaatgtaa cacttgaaaa
    14221 atctgacggc agttacttcg ggaaaaagag ttatcagatc acattcagta aagagaatta
    14281 tcagcccgta acgctgccga tcaaggcatc ggctaatgga tggtacattg gtggtaactt
    14341 tgtctttggt ggtattgttg gctggttgct tgtggaccca ttcaatggcg gtatgtatac
    14401 actgagtccg gaaagtctga atcccgggct ggtacccaaa acttaatcaa taatctgctg
    14461 atgaataaaa cctgctgcgg caggtttttt ttcgcccgga gaaaggtgaa tggcgacgtt
    14521 acgtgaactg attatcaaaa tttcggcaaa ttcacagtca ttccagtcgg agatccagcg
    14581 ggcgtcccgt atgggcagtg aatattaccg gaccctgcag aatggcggac gtcaggctgc
    14641 tgcggcagcc agggagcagc gccgggctct ggctgagctg aacagccagt tgacagaaat
    14701 ccgcgcttcg gctgtcggaa tgaccggtgc gtttgctggt gcctttgcca ccggacacct
    14761 gatttcgctg gcggatgaat ggagctccgt gaatgcccgt ctgaaacagg cgtcgcagtc
    14821 atcggatgaa ttttcgtcat cacagaaagt gctgatggat atcagccagc ggacaggcac
    14881 cgcattttcg gataatgcgg ccctgtttgc ccgttcggct gcctcgatgc gtgaatatgg
    14941 ttacagtgct gatgatgtgc tgaaggtgac ggaggccatt tcgacagggc tgaaaatttc
    15001 cggtgccagt gcggcagagg cgggttcggt gatcacccag ttcagccagg cgctggcaca
    15061 gggggtgttg cgcggcgagg aatttaattc ggtcaatgaa agtggggacc ggatcattcg
    15121 cgcactggct gcaggcatgg gcgtggcccg taaggatctg aaggcgatgg cggacgatgg
    15181 tcaactgacg gcggataaag tcgttcccgc gttaatcagc cagctgggga tattgcgtga
    15241 tgaatatgca gccatgccgg aaacggtgtc tgacgggatc acgaaggtgg aaaacgcctt
    15301 tatggcctgg gtgggcggcg cgaatgaggc cagcggagcg acgaaaacgc tctccggcgc
    15361 actgaacggt gtggccggaa atattgatac cgtggcaaca gctgcgggtg tgctggttgc
    15421 tgtcggggtg gcccggtact ttggtaatct ggcttccgga gcgatgtctg ccacggcagg
    15481 acttgtgacc gctgcacgta atgaagtggc actggctgag gcccagttaa ggggaacgca
    15541 gattgccacg gcgcgggcaa gggcagccgt gtaccgggct cagcaggctg tggcggcagc
    15601 ccgcgggact gagatgcaga ttgcggcaga ggcccgtctg gcgatcacac aggaacgcct
    15661 gaacagaaat attgctgcca gaaccgccgc ccagaatgcg ctgaacagta caacggcggt
    15721 gggctcacgt ctgatgagcg gtgcgctggg actggttgga ggcgtacccg gactggtgat
    15781 gctgggagct gccgcatggt acacgctgta ccagaatcag gaacaggcca gggagtctgc
    15841 gcgccagtat gcactgacga ttgatgaaat cgcgcataaa acgccatcaa tgtctctgcc
    15901 tgaagcctca gataatgaag agcgaacacg ggaggcgctg acagagcaga accggctgat
    15961 tgatgagcag gccagccggg tgaaatccct gcaggaaaaa atcgccgggt atcagtatgt
    16021 gctggctaat ccgggctgga cgaccggtaa cggcttcatg ataaaccatc tgacatcggt
    16081 gaaaactgta acggaagagc ttgctcaggc aacagagcag tttgctgttg agcagtcccg
    16141 tctggcacag atgcaggaaa aagcgcagtc cattcaggat gtgcttgccg gactggaaga
    16201 gcgccgtgtt gtgttaattc gtcagcaggc agcagagcag aataaagcgt atcagtcact
    16261 gctggtcatg aacggtcagc atacggaatt caaccgcctg ctggggctgg gtaatgaact
    16321 gctgcaacag cgtcagggac tggtgaatgt accgctgcga ctgccacagg ccactctgga
    16381 tgataaacag cagagcgccc tgaataacac agagcgtcaa ctggccctgt cccggctgaa
    16441 aggggaagaa aaagagcgtg cccggctggg gtatgcggcg gatgaccttg gtctggtggg
    16501 ggatacgtat caggaggcga ggcagcgtta catccgtaat tcgatggaag cctggcgcaa
    16561 taatgaggcg aataaaccca aatcccgggc cggaaaatct gagacggaaa aagcggaaga
    16621 cagtttttcc cgtctgctga agcagcagaa ggaacaactg gcactggcag ggaagaatac
    16681 agaactggcg aagctgaagt accagacctc gcagggagag ctgaaaaccc tgacggagat
    16741 acagaagcag gagttgctgc gcaatgctgc cctgattgac cagaagaaaa tccgggagca
    16801 gttacgggcc cgggaggagg ctctgaaaaa tgataatgca gacgaaaggg catcaaatga
    16861 cgctgagctg ctggggtacg ggcagggtga gcgggtccgt gaacggatgc gggagctgca
    16921 gcagattcgt gacggctacc gccagaagga tgcggacctg cagtctcagt atcagacagg
    16981 ggatatcagt gaggattttt acagacaggc gctggcgcag aatgcgcagt atctgagtga
    17041 acgccttaaa gaccaggagg ctttttatgc cgaatcggat gcgcagcgtg ctgactggca
    17101 gaaagggctg caggaagggc taagtaactg ggtggacagc gcatcagatt acgcttcaca
    17161 ggcagcacag cttgcgacag acggtatctc agggatggtg aataacatca cggagatgct
    17221 gaacggaaat aaagtggaat ggcgcagctg ggctgcatca atcctgcagg aaatatcaaa
    17281 agttcttatg aatgcggcca ttgtcaacgg cattaagatg gccgcaaaca gtatgtccgg
    17341 tgcaggagga tttttcggca gtataggcaa ctggctgggt ggcgcggtgg ccaatgcaaa
    17401 aggcggcgtt tatacctcgg caaacctgag tgcatacagt aacagtattg tggacacgcc
    17461 cacgtacttt gcctttgcaa aaggggcagg gctgatgggg gaagccggac ctgaagccat
    17521 catgcccctg acccgggcag cggatggttc actcggcgtg cgtgcggtgg gcagtatgaa
    17581 cggcagtgcg ggtctggtgt attccccggt gtaccacatt gccattcaga atgacggcgc
    17641 taacgggcag atagggccgg aagcggcggg cacccttgtg caactgattg accagcgggt
    17701 acaggcggtg atgttatcca tgcgtcgtga cggaggaatg ctgagtggat gagattaaga
    17761 cccttcactg gtgtccccgg gaagggatgc aggtgacgga gaaaccgtcg gtggtgacgg
    17821 tgaagtttgg cgacggttat cagcaacgtc gtccggcagg actgaatgcg caactgaaga
    17881 cctttcaggt ggtttttcgg gtgacaacgg atgctgagcg ggaggcactg tccgcgtttc
    17941 tgtcatggca tggtggttac cgggcttttt tgtggaagcc cccgaaacat aaccggacgg
    18001 tcagggtggt gtgccgggag tggagtatta cggataacgc ccggtacagt gatttcagtt
    18061 gcacgataga gcaggtggtg aactgatgca ggatattcac gaagaaagcc tgaacgagtc
    18121 ggttaagtca gagcagtcac cgcgggtggt gctctgggaa atcgacctga cggtgcaggg
    18181 cggtgagcgg tattttttct gcaatgagct gaatgaaaaa ggggagccgg tgacctggca
    18241 ggggcgtgaa tatcaggcgt acccgattga gggcagcggc tttgagatga acggaaaggg
    18301 cagcagtgcc cgcccgtcgc tgacggtgtc caatctgttt ggccttgtca ccgggatggc
    18361 ggaggatttg cagagcctgg tgggtgccac ggtggtccgt cgccgggtgt atgcgcgttt
    18421 tctggatgcg gtgaattttg tggcaggcaa tcctgaggcc gacccggagc aggagctgac
    18481 ggaccggtgg gtggtggagc agatgtcatc gctgacggcc atgacggcct cgtttgtgct
    18541 ggcgacaccg acggagacgg acggggcgct gtttcccggt cgcatcatgc tggcgaacac
    18601 ctgtatgtgg gattaccggg gcgatgaatg cgggtataac ggtccggcag tggcggatga
    18661 gttcgacaac cccaccacgg atatccggaa ggacagatgc agcaagtgca tgcgcgggtg
    18721 tgaactgcgc aggaatgtcg gcaattttgg cggtttcctt tccatcaata aactttcgca
    18781 gtaaatcctg ttttatgaca cagactgaat cagcgattct ggcgcatgcc cggcggtgtg
    18841 cgccagcgga gtcgtgcggc ttcgtgataa gcaccccgga gggcgaacgg taccagccct
    18901 gcgtgaatat ctccgcagag ccggaggcgt attttcgtat tacaccggaa gactggctgc
    18961 aggcacaaat gcagggggag attgtggcgc tggtccacag tcatcccggt ggtctgccct
    19021 ggctgagcga ggcggaccgg cggctgcaga taaagagtgc cctgccctgg tggctggtct
    19081 gccggggtga cattcaccgg ttccgctgtg tgccgcacct gaccggacgg cgctttgaac
    19141 acggtgtgac ggactgttac accctgttcc gggatgcata ccatctggcg gggattgaga
    19201 tgccggattt tgtgcgtgag gatgactggt ggcgcaacgg tcagaacctg tacctggaca
    19261 acctggcaga
//
LOCUS       DAZGQL010000093        19246 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.093, whole genome shotgun sequence.
ACCESSION   DAZGQL010000093 DAZGQL010000000
VERSION     DAZGQL010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19246
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.093"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(102..2768)
                     /gene="cas3"
                     /locus_tag="ACHKIR_003894"
     CDS             complement(102..2768)
                     /gene="cas3"
                     /locus_tag="ACHKIR_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HFO9991571.1"
                     /translation="MEPFKYICHYWGKSSKSLTKGNDIHLLIYHCLDVAAVADCWWDQ
                     SVVLQNAFCRNEMLSKQKVKAWLLFFIALHDIGKFDIRFQYKSAESWLKLNPATPSLN
                     GPSTQMCRKFNHGAAGLYWFNQDSLSEQSPGDFFSFFDAAPHPYESWFPWVEAVTGHH
                     GFILHSQDQDKSRWEMPASLASYAAQDKQAREEWISVLEALFLTPAGLSINDIPPDCS
                     SLLAGFCSLADWLGSWTTTNTFLFKEDAPSNINALRTYFQDRQQDASRVLELSGLVSN
                     KRCYEGVHALLDNGYQPRQLQVLVDALPVAPGLTVIEAPTGSGKTETALAYAWKLIDQ
                     QLADSVIFALPTQATANAMLSRMEANASRLFTSPNLILAHGNSRFNHLFQSIKSRAFT
                     EQGQEEAWVQCCQWLSQSNKKVFLGQIGVCTIDQVLISVLPVKHRFIRGLGIGRSVLI
                     VDEVHAYDTYMNGLLEAVLKAQADVGGSVILLSATLPMKQKQKLLDTYGLHTDPEENN
                     SAYPLINWRGVNGAQRFDLLAHSEQLPPRFSIRPEPIYLADMLPDLRMLERMIVAANA
                     GAQVCLICNLVDVAQLCHQRLKELNNTQVDIDLFHARFTLNDRREKENRVISNFGKNG
                     KRNVGRILVATQVVEQSLDVDFDWLITQHCPADLLFQRLGRLHRHHRKYRPAGFEVPL
                     ATVLLPDGEGYGRHEHIYSNVRVMWRTQQHIEELNGASLFFPDAYRQWLDSIYDDAEM
                     DEPEWVIKGMDKFESAECEKRFKARKVLQWAEEYSLQDNDETILAVTRDGEMSLPLLP
                     YLQTSSGKQLLDGQVYEDLSHEQQYEALALNRVNVPFTWKRNFSEVVDEDGLLWLEGK
                     QNQDGWIWQGNNIVITYTRDEGMTRVIPANPK"
     gene            complement(3127..3861)
                     /gene="cysH"
                     /locus_tag="ACHKIR_003895"
     CDS             complement(3127..3861)
                     /gene="cysH"
                     /locus_tag="ACHKIR_003895"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFO9991572.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIRPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNEINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            complement(3935..5647)
                     /gene="cysI"
                     /locus_tag="ACHKIR_003896"
     CDS             complement(3935..5647)
                     /gene="cysI"
                     /locus_tag="ACHKIR_003896"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HFO9991573.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(5647..7446)
                     /gene="cysJ"
                     /locus_tag="ACHKIR_003897"
     CDS             complement(5647..7446)
                     /gene="cysJ"
                     /locus_tag="ACHKIR_003897"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HFO9991574.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAAIEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVDGKTLTLSEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEDGEVRVFIEHNDNFRLPAN
                     PETSVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKIYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            7765..8127
                     /gene="queD"
                     /locus_tag="ACHKIR_003898"
     CDS             7765..8127
                     /gene="queD"
                     /locus_tag="ACHKIR_003898"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HFO9991575.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHYYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            8205..9476
                     /gene="ygcN"
                     /locus_tag="ACHKIR_003899"
     CDS             8205..9476
                     /gene="ygcN"
                     /locus_tag="ACHKIR_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFO9991576.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLARFKTHPAVRPLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLLGDALRSCVNTGISVRGMDMALTGTQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWRHLRRHGLWHLAGDVIRSLRCL"
     gene            9467..9727
                     /gene="ygcO"
                     /locus_tag="ACHKIR_003900"
     CDS             9467..9727
                     /gene="ygcO"
                     /locus_tag="ACHKIR_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFO9991577.1"
                     /translation="MSVTRNLWRVADAPHIVPADSVERQTVQRLINACPAGLFSLTPE
                     GDLRVDYRGCLECGTCRLLCDEKTLQQWRYPPSGFGITYRFG"
     gene            9744..10319
                     /gene="ygcP"
                     /locus_tag="ACHKIR_003901"
     CDS             9744..10319
                     /gene="ygcP"
                     /locus_tag="ACHKIR_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HFO9991578.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     QIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(10466..11326)
                     /gene="ygcQ"
                     /locus_tag="ACHKIR_003902"
     CDS             complement(10466..11326)
                     /gene="ygcQ"
                     /locus_tag="ACHKIR_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFO9991579.1"
                     /translation="MNIAIVTINQEHAAMAGWLAAQDFSGSTLTHWQIEPQPMVAEQV
                     LDALVEQWQRTPAEVVLFPPGAFGDELSIRLAWRLHGASICQVTSLDIPTVSVRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAEINTMLPSGMLQLNIVPGALPDWLISIENLKNV
                     TRDPLAEARRVLVVGQGGEADNQEIAMLVEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKAVLEA
                     LVTTIHADCQ"
     gene            complement(11323..12102)
                     /gene="ygcR"
                     /locus_tag="ACHKIR_003903"
     CDS             complement(11323..12102)
                     /gene="ygcR"
                     /locus_tag="ACHKIR_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFO9991580.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGNIGPDVSLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEDAVLLETAADLRFAPE
                     FVARHIAEWQRQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDAPFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEITRETVAAEAPA
                     IQCLQLARPEQRRRAALIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(12080..13417)
                     /gene="ygcS"
                     /locus_tag="ACHKIR_003904"
     CDS             complement(12080..13417)
                     /gene="ygcS"
                     /locus_tag="ACHKIR_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9991581.1"
                     /translation="MNTSPVRMDDLPLNRFHCRIAALTFGAHLTDGYVLGVIGYAIIQ
                     LTPAMQLTPFMAGMIGGSALLGLFIGSLVLGWISDHIGRQKIFTFSFLLITLASFLQF
                     FATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFSPRRHRGVLLGAFSVVWTVGYVLAS
                     IAGHHFISESPEAWRWLLASAALPALLITLLRWGTPESPRWLLRQGRFAEAHAIVHRY
                     FGPHVLLGDEVATATHKHIKTLFSSRYWRRTAFNSVFFVCLVIPWFVIYTWLPTIAQT
                     IGLEDALTASLMLNALLIVGALLGLVLTHLLAHRRFLLGSFLLLAATLVVMACLPSGS
                     SLTLLLFVLFSTTISAVSNLVGILPAESFPTDIRSLGVGFATAMSRLGAAVSTGLLPW
                     VLAQWGMQATLLLLAAVLLVGFVVTWLWAPETKALPLVAAGNVGGANEHSVSV"
     gene            complement(13510..14964)
                     /gene="ygcU"
                     /locus_tag="ACHKIR_003905"
     CDS             complement(13510..14964)
                     /gene="ygcU"
                     /locus_tag="ACHKIR_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HFO9991582.1"
                     /translation="MSLSRAAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQVTAQCGVPLEVLENTLREKGYTTGHSPQSKPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGPDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYVLEDMKTGFNILREIMVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNHLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            complement(15034..15819)
                     /gene="ygcW"
                     /locus_tag="ACHKIR_003906"
     CDS             complement(15034..15819)
                     /gene="ygcW"
                     /locus_tag="ACHKIR_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO9991583.1"
                     /translation="MSIESLNAFSMDFFSLKGKTAIVTGGNSGLGQAFAMALAKAGAN
                     IFIPSFVKDNGKTKEMIEKQGVEMDFMQVDITAEGAPQKIIAACCERFGTVDILVNNA
                     GICKLNKVLDFGRADWDPMIDVNLTAAFELSYEAAKIMIPQKSGKIINICSLFSYLGG
                     QWSPAYSATKHALAGFTKAYCDELGQYNIQVNGIAPGYYATDITLATRSNPETNQRVL
                     DHIPANRWGDTQDLMGAAVFLASQASNYVNGHLLVVDGGYLVR"
     gene            16138..17415
                     /gene="yqcE"
                     /locus_tag="ACHKIR_003907"
     CDS             16138..17415
                     /gene="yqcE"
                     /locus_tag="ACHKIR_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HFO9991584.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSASLKTVIIIYSVVYILLGILCWFFVSDNNNLRSTNNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSVLGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTLPAAEAYRNMWL
                     MGMAALGMVIVFTFLLFQKIRTADSAPAMASSK"
     gene            17442..18920
                     /gene="ygcE"
                     /locus_tag="ACHKIR_003908"
     CDS             17442..18920
                     /gene="ygcE"
                     /locus_tag="ACHKIR_003908"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HFO9991585.1"
                     /translation="MSKKYIIGIDGGSQSTKVVMYDLEGNVVCEGKGLLQPMHTPDAD
                     TAEHPDDDLWTSLCFAGHDLMSQFAGNKEDIVGIGLGSIRCCRALLKADGTPAAPLIS
                     WQDARVTRPYDHTNPDVAYVTSFSGYLTHRLTGEFKDNIANYFGQWPVDYKSWTWSED
                     AAVMDKFNIPRHMLFDVQMPGTILGHITPQAALATHFPAGLSVVCTTSDKPVEALGAG
                     LLDDETAVISLGTYIALMMNGKALPKDPVAYWPIMSSIPQTLLYEGYGIRKGMWTVSW
                     LRDMLGESLIQDARAQDLSPEDLLNKKASSVPPGCNGLMTVLDWLTNPWEPYKRGIMI
                     GFDSSMDYAWIYRSILESVALTLKNNYDNMCNEMNHFAKHVIITGGGSNSDLFMQIFA
                     DVFNLPARRNAINGCASLGAAINTAVGLGLYPDYATAVDKMIRVKDIFIPIESNAKRY
                     DAMNKGIFKDLTKHTDVILKKSYEVMHGELGNADSIQSWSNA"
BASE COUNT         4687 a         4974 c         4726 g         4859 t
ORIGIN      
        1 gggaaattaa aagaagatgt acattgtgca ctttccctac ttaagtaggg ataaaccgtt
       61 attggtcttg ttatcgtcat tgataccaat cattcccgaa gttatttggg atttgcaggg
      121 atgactctgg tcatcccttc atcccgagtg taggtaataa caatattatt accctgccag
      181 atccatccat cctgattctg tttcccttcc agccaaagta acccatcttc atctactact
      241 tcagaaaaat tacgtttcca ggtgaagggt acattgacgc gattaagtgc taacgcctca
      301 tactgctgtt catgacttag gtcctcgtag acctggccat cgagcagttg tttacctgaa
      361 gacgtttgta aataaggcaa taatggcagg ctcatttccc catccctcgt taccgcaaga
      421 attgtttcat cgttatcctg caagctatat tcttcagccc actgcagaac cttgcgagcc
      481 ttgaacctct tttcacactc agcgctttca aacttatcca tgcctttgat gacccattct
      541 ggctcatcca tttcagcatc atcgtaaatg ctatccagcc attgccggta ggcatcgggg
      601 aaaaataagg atgctccatt aagttcctca atatgttgct gcgtccgcca catgactcta
      661 acgttgctat aaatatgctc atgtcgtccg taaccttcgc catcaggcag caaaacggtg
      721 gcaagaggaa cctcaaaacc agcgggacga tatttgcgat gatggcgatg taaacggccc
      781 aatcgttgga aaagtaaatc tgcggggcaa tgctgagtaa ttaaccaatc aaaatcaacg
      841 tcgagtgatt gttccacgac ctgggtggcg acaagtatcc gtccaacatt tcgcttccca
      901 tttttgccga aattgctaat aactcgattc tctttttcac gacgatcgtt cagcgtaaag
      961 cgcgcatgaa acaaatctat atctacttgc gtgttattta gttcctttaa ccgttggtgg
     1021 catagctgtg caacatcaac caaattgcag ataagacaga cttgtgcacc cgcatttgcc
     1081 gctacgatca ttcgctctaa catcctaagg tcaggtaaca tgtcagctaa ataaataggt
     1141 tctggccgaa tcgaaaagcg gggcgggagt tgttctgaat gagctaacag atcaaaacgt
     1201 tgtgcaccat tcacacctcg ccagttaatg agtggatatg cggagttatt ttcctctgga
     1261 tctgtatgca gaccataagt atccagaagt ttctgttttt gtttcattgg tagagttgcg
     1321 gaaagaagaa taacactccc ccccacatca gcctgagcct tgagcacagc ctccagcaag
     1381 ccgttcatat acgtgtcgta agcatgaact tcatcaacaa ttaaaacact tcgaccaatt
     1441 cccaaaccac ggataaagcg gtgtttaact ggcaataccg atatcaacac ctgatcaatc
     1501 gtgcaaacgc cgatttgccc aagaaacact ttcttattgc tttgtgacaa ccactgacaa
     1561 cactgaaccc atgcttcttc ttgcccctgc tcagtaaatg cgcgtgattt tattgattga
     1621 aaaaggtggt taaaacgtga attaccatga gcaagaataa gatttgggga ggtaaataaa
     1681 cggctcgcgt tcgcttccat tctgctgagc attgcatttg cagtagcttg tgtagggagg
     1741 gcaaaaataa cactatccgc aagttgttgg tcaataagtt tccaggcata ggccagcgca
     1801 gtttcagttt tacctgaacc cgtaggtgcc tctattaccg tcagcccggg cgctactgga
     1861 agagcatcaa ctaacacttg taattgtctg ggttgatagc cattgtccag tagagcatga
     1921 acaccttcat aacatcgctt atttgataca aggccgctca actccaatac ccggctcgca
     1981 tcctgctgtc ggtcctggaa atacgttctt agagcattta tgttggaagg tgcatcctct
     2041 ttaaacagaa aggtattcgt tgtagtccag gagcctaacc agtcagcaag cgagcaaaaa
     2101 cctgctaaca gtgatgaaca atcaggtggt atatcgttta tagataaccc ggctggcgtt
     2161 aaaaataatg cttcgagtac agatatccac tcctcacgag cctgtttatc ttgcgcagca
     2221 taagatgcca gagaagctgg catttcccaa cgcgacttat cttgatcctg ggaatgtaat
     2281 ataaaaccat gatgtcctgt aacagcctct acccatggaa accaggactc ataaggatga
     2341 ggagcggcat caaaaaaact gaaaaaatcc ccgggagatt gctctgaaag tgaatcctgg
     2401 ttaaaccaat acagaccggc tgcaccatga ttaaatttac ggcacatttg tgttgatgga
     2461 ccattaagtg atggcgttgc aggatttaat ttcagccaac tttctgctga tttatattgg
     2521 aatcgtatat caaactttcc gatatcatga agggcaatga aaaataacag ccaggctttc
     2581 accttctgtt ttgatagcat ttcatttcgg caaaaagcat tttgtagtac gactgattga
     2641 tcccaccagc aatctgcaac agctgcaaca tccaggcaat gataaatcaa cagatgaata
     2701 tcatttcctt tcgtcaggct ttttgaggat ttcccccagt aatgacatat atatttaaaa
     2761 ggttccatta atagcctccc tgttttttta gtattatcga tgatatcagt ggactatttt
     2821 tcgatgctgc tcataaatat cgaattacag tgatatacac atgtcttgcc tccctgcaaa
     2881 gaagagacta acattcgaat tgcttaggtt cgaacgctgg cctcaggttg atagaaatat
     2941 cgcctggggc ttttgtccat ctggaacctc gcgaatgctt aacgccagac agcctcaagc
     3001 acccaactcc attctatacc tgttaattct ccctgcgatg ttcaccttta aatgccggaa
     3061 tgctgctcgc aaaataaaaa cgcccccatc atttctgaca gaggcgttta atttatccgg
     3121 caatatttac ccttcgtgta acccacattc cctttttaag ccaaagaaac gcgtttcttc
     3181 ttccgccatg ccgggttccc atttacgggt tgtatgggta tcaccgaccg ataaatatcc
     3241 ttcgtcccat aatgggtgat atttcaggcc atgtttttgc aggtactgat aaatagttcg
     3301 gttatcccag tcgataatcg gcagcacttt aaatacgcca cgctgaattg ccagcaccgg
     3361 taaattggcg cggctgccgg attgttcacg gcgcaggcca gcaaaccagg tttgcgcatt
     3421 cagttctttc agcgcccggt tcatcggttc gactttgttg atctcgttgt acttctcaat
     3481 cccctcaaca ccctgctccc acagtttacc gtagcgcgct tcctgccagg cggcgctttc
     3541 ggtagcgcgg tacactttca ggttgagctt gagtttgtcc gttaactcgt caataaagcg
     3601 gtaggtttct gggaacagat agccggtatc ggtgaggatc accggaatat ccgggcgaat
     3661 ctgattcacc agatgcaggc tcaccgccgc ctgaatgcca aagctggaag aaagtacata
     3721 ttcaccgggc agattatcca gcgcccaggc aacgcggcct tcagcgtcca gtttttccag
     3781 ttcggcgtta gtttccgcca gcgccagaat gcgatctacc ttcggcagtt cgttcagggc
     3841 gtttagatcg agtttggaca tagattcctc actgtttgcc ttgcctgatg cgacgctcac
     3901 gcgtcttatc aggcctacaa gaccgggcta atggttaatc ccacaaatcg cgcgccggat
     3961 cgagcaccgg gcgaatgatg cccgcacgca ccgtaaagtc gccgaagcct tcacccgctt
     4021 cgcgctcttt cgcccagcgc cctatcagtt catcaagcga cgccaggatt tccggctcgg
     4081 tgatgttttc tttatacatc cgtgggatac gtgtcccaat gcggttgccg ccaagatgca
     4141 agttgtagcg acctggcgct ttacccacca ggcccacttc cgccagcatc gcgcgaccac
     4201 aaccgttcgg gcagcctgtt acacgcatca cgatatgctc atcgctgaca ccatgtttcg
     4261 ccattaaatt atcgatgttg tcgataaaag acggcaggaa acgctcggct tcggccatcg
     4321 ccagcgggca agtcgggaat gacacgcagg ccattgagtt ttcacgctgc ggcgtgacgg
     4381 cattcattaa cccgctctct ttggcgatct tctcgatctt cgctttctcg ctttccggca
     4441 caccggcgat gatcagattc tggttcgccg taatgcggaa atcgcctttg tggatcttcg
     4501 cgatctccag caggccggtt ttcagcggac gcccaggata atcaaggatg cgaccatttt
     4561 cgataaacag cgtcagatgc cagttatcat caatgccctt aacccagcca atacgatcgc
     4621 cgcgtccggt aaactcatac ggacggatcg gttcaaattt gatccccgcg cgacgctcca
     4681 cttccgcttt aaacgtctca acccccacgc gctccagcgt gtatttggtt ttggcatttt
     4741 tacgatcggt tcggttaccc cagtcacgct gagttgtcac gacggcttcc gccactgcca
     4801 acgtatgctc cagcggcaga tagccaaact cactcgcggt gcgggcgtag gttttcttgt
     4861 tgccgtgttc gatggaaagc ccaccgccca ccaacaggtt aaagcccacc agcttgccgt
     4921 tttcggcgat tgccacgaag ttcatgtcgt tggcgtgcag atcgatatcg ttctgcggcg
     4981 ggatcactac cgtggttttg aacttacgcg gcaggtaggt ctgaccaagg atcggctctt
     5041 catcagtagt ggcgactttt tcctgatcga gccagatctc cgcatacgcg cgggtacgag
     5101 gcagcagatg ttcagagatt ttcttcgccc attcatatgc ttccgcgtgc agttgcgact
     5161 cgtaagggtt cgaggtgcag agtacgttac ggttcatgtc gttagcagtc gccagcgcat
     5221 caaggccgac cgagtgcagc atctggtgca ccggtttgac gttctttttc agaatgccgt
     5281 ggaactgaaa cgtctggcgg ttggtcaggc gaatgctgcc atagatggtg ttttcaccgg
     5341 caaatttgtc tatcgcctgc cactgtttgg tggtgatcac gccacccggc agacggcagc
     5401 gcaacagcat cgcgtggcgc ggctccagct tctgttcagc acgttcggcg cggatgtcgc
     5461 ggtcatcctg ctgatacatg ccgtggaagc ggatcagcag gaagttgtcg cccttaaagc
     5521 cgccggtcag accgtcgttt aaatcttccg caatggtgcc gcgcaggtag ttgctttcaa
     5581 gcttcatgcg ctcggcgtct gtcagttttc cttcgaccac taaaggccct ggatgttttt
     5641 cgctcattag tagacatctc gctgataacg gcgctctacg cgcagctcac ttaaaaattc
     5701 atccgccgct tcggtgtcca tgccaccaaa ttcggcaatc acttccagaa gtgcctgctc
     5761 aacgtctttc gccatgcgat tagcgtcgcc gcagacataa atgtgggcac cgtcattgat
     5821 ccagcgccac agctccgcgc cctgttcgcg cagtttgtct tgtacgtaaa ttttttcttt
     5881 ttgatcgcgc gaccaggcaa gatcgatacg cgtcagcacg ccctctttga cgtagcgctg
     5941 ccattccacc tggtagagga aatcttccgt aaagtgcggg ttgccaaaga acagccagtt
     6001 tttccccggc gcctcgtcgg cggcgcgttg ctgcataaag gcgcggaacg gcgcgatgcc
     6061 ggtgcctggg ccaatcataa tcaccgacgt ttccggattg gctggcaggc ggaagttgtc
     6121 gttatgttcg ataaagacgc ggacttcacc atcttcttcc acacgatctg cgaggaagct
     6181 ggaggcacca ccggcacggg cgcggccttc cacgtcgtaa cgcaccacac caacggtaac
     6241 gtgcacttcg ttctcgactt ccgcctgcga ggaggcgatg gaatacaggc gcggggtcag
     6301 cgggcgcagc aggttaatca gcgcttcggc atccagttgc gccggagaga aacgcaccat
     6361 gtcaacaatc ggcgtggtcg cggcgtaatg ctgtaacttc gctttatcgc ccaccagcgg
     6421 cagcagcgtt tcgctgcggg taagcgtggc gtagttctca acaatattcg cggtgttgac
     6481 ggtcagttca aagtgccact gtagcgcttc gctcagggtc agcgttttgc cgtcaacggt
     6541 gacaggttca tcgcctttca gccacagcag ttcgacaagt tctttcacca gtgccggatc
     6601 gttctgatac cagacgccca gcgcgtcacc cggctggtaa cgcaggccag agtcacctaa
     6661 gtcaatttcg atatggcgaa cgtctttttc agagttacgc ccggtaattt tctggttaac
     6721 agagaggctc gccaccagcg gcgcgtcttt gctgtacggg ctggtgtgga tttcatttac
     6781 cgcgccagta gcgacggatt gcgaaggtgc cgcgacaggc gcacgcgatt taagcgcatc
     6841 aaccacgcgg gcgcgccact cgatggcagc agcctggtat tcaacgtcgg catcgacacg
     6901 gtcgagcagg cgttcaccgc ccagttccgc cagcttactg tcaaaatctt tcccggactg
     6961 gcagaaaaat tcataagaac tatcgccgag gctaaacacg gcaaaagcgg tattttccag
     7021 cttcggcgct tttttggaga acaggaactt atgcagcgcg acggcttctt ccggcggctc
     7081 cccttcccct tgcgttgacg tcactacgat gagcagtttt tcgctggcga tttgtttgaa
     7141 tttatagtcg cccgcgttca ccagcttaac gttcagtttt gctgctaata aatcatcgcg
     7201 taatgcttca gcaacccggc gcgcattgcc ggtttgcgag gcggagataa tagttatacc
     7261 cggcatttct gcggctggcg ctggcgtcgc tgcaagcgca gcaggctgct gattgagtac
     7321 gccccagaaa tagccagaaa cccaggcaag ctgggtagga gttaaatcgg tcgtggccgc
     7381 ctgaaggcgt gccagttgct ccgggttcaa cggaagcaac gcggaaggtg ggacctgtgt
     7441 cgtcatgcgt cgttatgttc cagtaagcaa agctgtttct gcgccctgtc agcgcccata
     7501 aaacagaaga ggaggtaagg ttaacggggt gaacggtgtg gattaaagac gggatagcga
     7561 taactaataa ccaaatcgac taacctgttt tagcaatagt ctttaacaac aaaacagatt
     7621 aaccaaccca atgaaaaaca aatgaattta gccaatcatt aagataaatc agcgattttg
     7681 cgcaacaaag tcgttttaga taatgcgaaa aaacagcctt tccggtactc tacggcggtt
     7741 ttatcgtcct gtagagaaat tatgatgtcc accacgttat ttaaagattt caccttcgaa
     7801 gccgctcacc gcttaccaca cgtcccggaa gggcataaat gtggtcgcct gcacgggcat
     7861 tcctttatgg tgcgactgga aattaccggg gaagtcgatc cgcatacggg ctggattatc
     7921 gatttcgctg aactgaaagc ggcgtttaag ccaacctacg agcgcctcga tcactattat
     7981 ctcaatgata ttccaggtct ggaaaacccg accagcgagg ttttagcaaa atggatttgg
     8041 gatcaagtta aacccgttgt gccgctgtta agtgcggtga tggtaaaaga aacctgcacc
     8101 gcaggttgca tctatcgcgg cgaatgataa gagtgtgtcg gcggtcaatt tcccttaagt
     8161 aacgctatgt tagggtgttg tgttctggat atctggggca tgacatggaa gacgactgcg
     8221 acattattat tattggtgcc ggtattgcag gcaccgcttg cgcgttacgc tgcgcgcgag
     8281 cgggtttatc cgttttgtta ctggaacgcg ctgaaatccc cggcagcaaa aatctttccg
     8341 gcgggcggtt atatacccat gcactcgcgg aactcctccc tcagtttcac ctgaccgcgc
     8401 ctcttgaacg acgcatcact cacgaaagcc tttccctgtt aacgccggat ggcgcaacga
     8461 cgttttccag cttacagccc ggcggtgaat cctggagtgt attacgtgca cggttcgatc
     8521 cgtggctggt tgccgaagcc gaaaaagaag gtgtcgaatg catccccggt gcgacggtgg
     8581 atgcgctgta tgaagaaaac ggcagggtgt gtggtgtcat ttgtggtgac gatattctcc
     8641 gcgcccgtta tgtggtgctg gcagaaggtg ccaacagcgt cctggctgaa cgtcacgggt
     8701 tagtgactcg tcctgctggc gaagcgatgg cgttggggat caaagaagtg ctgtcgctgg
     8761 aaacatccgc tattgaagaa cgttttcatc tggagaataa cgaaggcgca gcgttgctgt
     8821 tcagcggcgg gatctgtgat gacttacccg gcggcgcatt tctttatact aatcaacaaa
     8881 cgctctcgtt agggattgtt tgcccgctct cttcccttac gcaaagtcgt gttccggcaa
     8941 gcgagctgct ggctcgcttt aaaacgcatc cggcagtgcg cccgcttatc aaaaacacgg
     9001 aatcactgga gtatggtgcg catctggtgc cagaaggtgg cttgcacagt atgccggtgc
     9061 aatacgccgg taacggctgg ctgctgctgg gcgatgcgtt gcgcagttgc gtcaataccg
     9121 gaatttccgt gcgcggcatg gatatggcgc tgactggcac gcaggcggcg gcacaaacgc
     9181 tgataagcgc ctgccagcac cgcgagccgc aaaatctgtt tgcgctttat catcacaacg
     9241 tagagcgcag cctgctatgg gatgttctac aacgttatca gcatgttccg gcgcttttgc
     9301 aacgccctgg atggtaccgt gcgtggcctg cgttaatgca ggatatttcc cgcgatttat
     9361 gggatcaggg tgataaacct gttccaccgc tgcgccagtt attctggcgt catttacgtc
     9421 gtcacggcct gtggcatctg gcgggcgatg ttatcaggag tctgcgatgt ctgtaacccg
     9481 taatctctgg cgcgttgccg atgcgccgca cattgtaccg gctgactccg ttgagcgtca
     9541 gacggtacaa cggttgatta acgcttgtcc ggctggtctt ttttcgctca caccggaagg
     9601 tgacttacgc gttgattatc gcggctgcct ggagtgtggc acctgccgtt tgctgtgcga
     9661 tgaaaaaaca ctacaacagt ggcgctatcc gccttccgga ttcggcatca cctaccgttt
     9721 tggataaata ataaggataa tttatgcccc tcttacacct gctccgccag aatccggtga
     9781 ttgctgccgt taaagataat gccagcctgc aactggcaat cgactctgaa tgccagttta
     9841 tttccgtgtt gtacggcaat atctgcacca ttagtcaaat cgttaaaaag attaaaaatg
     9901 ccggaaaata tgcttttatt catgttgatt tgctggaagg cgcgtcaaat aaagaagtgg
     9961 tgattcagtt tttgaaactg gtgactgagg cggacggcat tatcagtacc aaagcctcaa
    10021 tgttgaaagc ggcaagagcg gaaggttttt tctgtattca tcgcctgttt attgttgatt
    10081 cgatttcgtt tcacaacatt gataagcaag ttgcgcaatc gaatccggat tgtattgaga
    10141 tcctgccagg ctgtatgcct aaagtgctgg gctgggtgac ggagaaaatc cgccaaccgc
    10201 tgattgccgg cgggctggtg tgcgatgaag aagatgcgcg taatgcgatt aacgcgggtg
    10261 ttgtggcgct ttccaccacg aataccgggg tctggacgtt agcgaaaaaa ttgctttgac
    10321 gggatactcg taacgagttg aatttgtgtg atttgtaggc tgcacgccac atccgacatt
    10381 cagcgcctga tacgacgctt gacgcgtctt atcaggctta caagtcccca gcccagaacc
    10441 gtaggcagga tcatccggca gcacgttatt ggcaatctgc gtgaatggtt gtcaccagtg
    10501 cctccagcac cgctttccag tcatcaacca cgccaacatc tgcctgtgaa aataccgccg
    10561 cgctggcgtc atgattaatc gccaccacaa acttgctatt gcgtacgccc gccattaacg
    10621 ctgctgcgcc ggaagcgcca acaacaatgc acacctcagg cgccagcaga tgtccggaaa
    10681 taccaatcac tttttcagca tcaacgccac cgttcatcac tcgcgcccga ctgtagccca
    10741 cttcggctcc cagcttttct accagcatag cgatttcctg gttatctgcc tctccgccct
    10801 gccccaccac cagcacccgt ctggcttcag ctaatggatc gcgggtaaca tttttcaggt
    10861 tttctatgct aatcagccag tcgggaagcg caccggggac aatgttcagt tgcagcatac
    10921 cagagggcaa catggtgttt atctctgctc ccgcctgacg cgccagggaa agacatagcg
    10981 ggcgtttttc tgtttgcagc gtcgccgtta gcgcatttcc ccagtgggat ttgcgcacgc
    11041 ttacggttgg aatatctaac gaagtcacct ggcagatgct tgcaccatgt aaacgccagg
    11101 ccagccggat cgacagttca tcgccaaacg cgcccggagg aaaaaggaca acctccgcag
    11161 gcgttcgttg ccactgttcc accagcgcat ccagcacctg ttccgcaacc atcggctgtg
    11221 gttctatttg ccaatgcgtt agtgtgctgc cgctgaaatc ctgcgccgcc agccagcccg
    11281 ccattgcggc atgttcctga tttattgtca caattgcgat attcatggct gcatcctttg
    11341 gcgtaaatag tcctgccata gcttttgcgc tttttctgct accgtttgcc catcaatcag
    11401 cgccgctctg cgtcgttgct ctggccttgc cagttgcagg cactgtatcg ctggcgcttc
    11461 cgcggcaaca gtttcgcggg taatctccgc tttccccgct gccattcgct ggcgcattcc
    11521 cggtactggc aaggccactt caccacactg acgcacggca atcaccgcag gcaagcgaac
    11581 ccggcaacag cgcaacccat gttcagtacg ctgttcgagg gtgataaacg gcgcgtcgag
    11641 agtaaaacgc tccacctggg tgaaacaggg ccagcccagc atttccgcca gcaaaaatgg
    11701 cgtttgtcca ttctgccctt cgctgctttg gcagccggta atgatcagat ccagcggatt
    11761 ctgacgctgc cattcggcaa tatggcgagc gacaaattcc ggcgcaaagc gcagatctgc
    11821 cgccgtctcc agcaatacag cgtcctcaaa cccaagagcc atgagatagc gcagccagtg
    11881 caacgcccgt tcatccccca tgctcaacgc ggttaaagac atcggcgtac cgttttttct
    11941 ctgcgccaac agcagcgcgg cggcagcctg ttcatcggca ccgagtaaac ttcgcagaag
    12001 cgaaacatcc ggtccgatat taccctgagc cgccgcctgc cactcttttt ccgccagcat
    12061 tccggcatcc ggttcggctt taaacgctaa cagaatgttc attcgcacct cctacatttc
    12121 ccgccgccac cagcgggagt gctttggttt ctggtgccca tagccaggtc acaacaaaac
    12181 caaccaacaa cactgccgcc aggagcaata aggtggcttg cattccccac tgcgccagca
    12241 cccacggcag caggccagta cttaccgccg cccccaaccg actcatggcg gtggcaaaac
    12301 cgacgcccag cgagcgaatg tcagtaggaa aactttccgc aggcaaaatg cccaccagat
    12361 tactgactgc cgaaatggtg gtgctgaaga gaacaaaaag cagcagcgtt aatgaactgc
    12421 cggaaggcaa acaggccatc acgaccagcg ttgccgccag cagcaaaaaa cttcccagta
    12481 aaaatctgcg atgcgctaac aagtgggtca acactaaccc taataacgcg ccaacaatta
    12541 acaacgcatt aagcatcagg ctggcagtca gcgcatcttc cagaccaata gtttgggcga
    12601 tggtcggcag ccaggtataa atcacaaacc atgggattac gaggcagaca aagaagacgc
    12661 tgttaaacgc tgtgcgccgc cagtagcgcg aagagaacaa ggttttgatg tgtttatggg
    12721 tcgccgttgc cacttcatcg cccagtaaaa catggggacc aaaatagcga tgcacgatag
    12781 cgtgagcttc tgcaaaacgc ccctggcgca gtagccagcg tggcgattct ggcgttcccc
    12841 agcgtaataa cgtaatcaac aatgcgggca gagctgccga tgccagtagc cagcgccagg
    12901 cctccgggct ttcgctaata aagtgatgtc cggcaatact tgccagcaca tagcctacgg
    12961 tccacaccac gctgaatgcg cccagcaaaa caccgcgatg gcggcgcgga gaaaattcag
    13021 ccagcaaagt gtgaccgact gagtaatcgc ctcccagacc aatgccgatc aaaatgcgca
    13081 gtccaataag atgctctggc gtggtggcaa aaaattgtaa gaacgaagcg agcgtaatca
    13141 gcaaaaagct gaaggtgaag attttttgcc gaccaatatg gtcggagatc caccccaaaa
    13201 ccaggctacc gataaacagg ccaagtagcg ccgagccgcc gatcattccc gccataaacg
    13261 gcgtcagttg catggcgggt gtaagctgaa taatggcgta accaatgacg ccgaggacat
    13321 aaccgtcggt caggtgtgcg ccgaaagtga gcgcagcaat gcggcagtga aaacggttaa
    13381 gtggtaaatc atccattcgc accggtgaag tgttcatctt taccccttca ttgtccggta
    13441 ataagaacgg cttctcgcct gagaagccga aaaggcaacg cccgtcatcg cggaacgctg
    13501 cctgacacat tatttttcaa tcggatagat agtacccgta ttcataatgc cgttaggatc
    13561 gaactgcttt ttcagccctt ccagcaacgc ccacgcgctg ccgtgttcca gtttgctcca
    13621 gtgaacgcga tgtttaccga taccgtggtg gtgcaccatc gaaccaccga ggcgaatggt
    13681 ttcttcgcag atgattttgt tgagcggatt gtggtacttg tcgatttcct cttccggctt
    13741 acagtcaacg acgttgtagt cgtagacgaa gtacatgttg gtgccgttct gatagctatg
    13801 agaggagtga ccgcccagca tggtgatgtc gtcggcatgc gggaactcgg tacgaatacg
    13861 gttaataacg ctttcgtaga tttcgtggat gcagctccag cagccggaca cttcggtggt
    13921 aaagcccatg ttgccggttt tgaggatctg cacacgttca gcggccactt tatccggccc
    13981 ccagttcagg tgattaaacc aggtttcgat cagcttgctg tccactcgct ggcattgcgg
    14041 gtaacgtgcc acgatttcag caatcccttc gcctgtcgcc ttcgcaatgc gagggttacc
    14101 ttcagccata aagatcagca cacatttacc atcggcaaaa tgggtgaagt gttgggtgcc
    14161 atcttcagcg tcatacaaac gagcgatcga cggacgatac ccttccacca tgatttcacg
    14221 caggatgttg aagccggttt tcatgtcttc cagcacatag ccatagaaga gattgttttc
    14281 cggcgtaaat ttgaagattt tcactgtgac ttcagtgata tagcacaatg caccttcgtt
    14341 gccgatgata atgtgacgaa tgtccgggcc tgccgcgcgg cgtggtacgt ttttaatacg
    14401 tgtgacggtg ccatcagcca atactgcttc cagaccaacg accatatctt cgattgcgcc
    14461 gtagagtgtg gagaactgcc cgatactgcg ggttgctacc aggccgccca tctgcgccag
    14521 cggttttgat tgcggtgaat gccccgtggt gtaacctttt tcacgcaacg tgttttccag
    14581 cacctccagc ggaacaccac attgcgccgt gacctgcata ttctcaatat caatattaat
    14641 gatttgattc atggcagagc cgtcgagcac caccgagttt tccacaacgg tttccagtcc
    14701 accctcggta gcggaagcac cggtacgcgg cacaccgtta attttgtgtg cattcataaa
    14761 attcagtaca cgagagactt gttctgtgga accgagtttt acgaccgctg ccggaatcgg
    14821 caaagtataa atgccatgaa tatccggaaa tttgcgaaaa cggtcaatac tgtttttctt
    14881 taatactgtt tcatcggtaa ttacgcgatc tgcaccaaca atttccttta gctggtcgac
    14941 aatcgctgcg cgagataaag acatagtaat tccttcctga taataaaaat ggaataatca
    15001 ttaataagag aaaacagaaa ccaccgttat ggattagcgc actaaataac cgccatcgac
    15061 cactaataaa tgcccgttga cataattcga tgcctgactt gcgagaaaca cggctgcgcc
    15121 cattaaatcc tgagtatcgc cccaacggtt tgccggaata tgatcaagaa cgcgctgatt
    15181 ggtttctgga ttactgcgtg tcgccagcgt aatatctgtt gcataatagc caggggcaat
    15241 accatttacc tgaatattat attgacctag ttcatcacaa taagctttgg tgaacccggc
    15301 aagagcatgt ttagtggcag aatatgcagg tgaccattgt ccacctaagt aagagaacaa
    15361 tgaacagata ttaataattt taccgctttt ctgcgggatc ataatttttg ccgcttcata
    15421 gcttaactcg aatgcggcgg tcaggttcac atcaatcatc gggtcccagt cggcacgacc
    15481 gaaatccagc accttattca gcttacaaat accggcattg ttaaccagaa tatcaactgt
    15541 tccgaaacgc tcacagcaag cagcgataat cttctgcggc gcgccttctg cggtgatatc
    15601 cacctgcatg aagtccatct caacaccctg tttttcaatc atttcctttg ttttgccgtt
    15661 atctttaacg aagcttggaa taaagatatt tgcaccagct ttggccagcg ccatggcaaa
    15721 tgcctggcct aaaccgctat tcccgccggt aacaattgcg gttttacctt tcagggagaa
    15781 aaaatccatt gagaacgcat tgagagattc gattgacata gttagctcca atttccgtaa
    15841 ggcaaaaaaa aagaaaggta acttcccccg cgagagggaa gttacctttc tcattatctc
    15901 tggtaactgg agataatttt tatcataagg gcataatcgc taatgtgatc gtaatcacag
    15961 tgttatattc gaattgtcaa ataattttaa aaagcagaag cgtgattact ctcacacaac
    16021 accactgctt tccatgttat ttcttattgc aattactaga gacctgagaa aagtaatcca
    16081 cctgaaaggg cggcttactt ttctcttttt tttgtctatt acaaatggga atacgatatg
    16141 caacacaact catatcgccg ttggataacc ctcgcgataa ttagttttag cggcggcgtt
    16201 agtttcgatc tggcttattt acgttatatt tatcaaattc ccatggcgaa atttatggga
    16261 ttcagcaata ccgagatagg tttaataatg agtacctttg gtattgcggc tattattctt
    16321 tatgccccca gcggcgttat tgccgataaa ttttcacacc gcaaaatgat tacttccgcg
    16381 atgatcatta ccggattact gggtctgtta atggcaacat atccaccgct gtgggtaatg
    16441 ctctgtattc aggtcgcctt tgcgataacg acgattttaa tgctgtggtc ggtgtcgatt
    16501 aaagccgcct cattgctggg cgatcatagt gagcaaggga aaattatggg ctggatggaa
    16561 gggctgcgcg gcgtcggtgt aatgtcgctg gcggtgttta ccatgtgggt cttttctcgc
    16621 tttgcgccgg atgacagcgc cagcctgaaa acagtcatta ttatctacag tgtggtttac
    16681 atcttgttgg ggattctgtg ctggtttttt gttagcgata acaacaacct gcgcagtacc
    16741 aataacgaag aaaaacagtc attccagctt agcgatatcc tggccgtttt gcgtatcagt
    16801 accacctggt attgcagcat ggtgattttt ggtgtcttca ccatctacgc cattctgagt
    16861 tactccacca actatctgac cgaaatgtac ggcatgtcgc tggtggcggc gagctacatg
    16921 gggattgtga tcaacaaaat cttccgcgcg ctgtgcggcc cacttggcgg cattatcacc
    16981 acctacagta aagtgaaatc ccctacccgc gtgatccaaa tcctttccgt actcggcctg
    17041 ctggcgttaa ctgccctgct cgtcacgaac tctaacccgc aatcggtcgc gatggggatt
    17101 ggcctgattt tactgctggg attcacctgt tacgcctcac gcgggctgta ctgggcctgc
    17161 ccaggcgaag cgagaacacc gtcttacatt atgggcacca cggtaggtat ttgttcggtg
    17221 attggattcc tgccggatgt cttcgtttac ccaattatcg gccactggca agacaccctg
    17281 cccgcagcag aagcctaccg caatatgtgg ctgatgggca tggcggcgct tggcatggtg
    17341 attgtcttta cctttttgct gttccaaaaa attcgtactg ctgatagcgc ccccgcaatg
    17401 gctagcagca agtaagccag gaaaattaca ggagaaaaat catgtcgaaa aaatacatca
    17461 tagggattga tggcggaagt cagagcacaa aagtggtgat gtacgatctg gaaggtaatg
    17521 tggtttgcga aggtaaaggc ttattgcagc cgatgcatac gccagatgcc gatactgccg
    17581 aacatcctga cgacgattta tggacatcgt tatgctttgc cggtcacgat ttgatgagcc
    17641 agtttgccgg aaataaagaa gatattgtcg gtattggtct gggctccatc cgttgctgtc
    17701 gtgcgttatt gaaagccgat ggcacgcccg ctgcgccatt gattagctgg caggatgcac
    17761 gtgtaacacg tccctacgat cataccaatc cagatgtggc ctatgtcacc tctttttcgg
    17821 gttatctgac gcatcgctta accggcgagt ttaaagataa tatcgccaac tattttggtc
    17881 agtggccggt ggattataag agctggacat ggagtgaaga tgctgcggta atggataagt
    17941 ttaatatccc ccgtcatatg ctgtttgatg tgcaaatgcc tggcaccatt cttgggcata
    18001 tcacaccaca agctgcgctg gcgacacatt tcccggctgg gctgtcggtt gtttgtacca
    18061 ccagtgataa accggtagaa gctctgggag ccgggttact ggatgatgaa actgcggtaa
    18121 tttctcttgg cacttacatc gcattgatga tgaacggcaa agccttgccg aaagatccgg
    18181 tggcgtactg gccgattatg tcttctattc cgcaaacatt gctgtatgaa ggttacggta
    18241 ttcgcaaagg catgtggacg gtgagctggc tgcgcgacat gttaggcgag tcgttaattc
    18301 aggatgccag ggcgcaggat ctttcaccgg aagatttact caacaagaaa gcctctagtg
    18361 tgccacctgg ctgtaatggg ctgatgacgg tgctggactg gctgaccaat ccgtgggaac
    18421 cgtacaaacg cgggattatg atcggctttg attccagtat ggattacgca tggatatatc
    18481 gttcgatact ggaaagcgtg gcgctgacgc tgaagaacaa ttacgacaat atgtgtaatg
    18541 aaatgaatca ctttgcgaag catgtaatca ttactggcgg cggttcgaac agcgatctgt
    18601 ttatgcagat ttttgccgac gtgttcaacc ttccggcacg acgtaacgcc attaacggtt
    18661 gtgcaagtct gggggcagcg attaataccg cggtaggtct ggggctatac ccggattacg
    18721 caacggctgt cgacaaaatg attcgcgtga aagatatctt tataccgatt gagagcaatg
    18781 ccaaacgcta cgacgcgatg aataaaggca ttttcaaaga cctaaccaaa catactgatg
    18841 tgatcctgaa aaaatcgtat gaagtgatgc atggggaatt ggggaatgcg gattcgatcc
    18901 agagctggtc gaatgcgtaa gtgaggaagg ccgggcggga aactgcccgg cctgaacata
    18961 cctgaatggt tatccccgct gacgcgggga acataagttc gacaacttaa aaaatgataa
    19021 caagtccggt ttatccccgc tgatgcaggg aacataataa aaactttaac tagttcggtt
    19081 tatccctgtt tttacaagga atattaacta cactcgatgt ctttagaata aaagacaata
    19141 cgaatcgtac tttatcaatt ttcatttaaa gtatgaactg gatatcttta ttcataagaa
    19201 caattcatca acagcgtata gaggcggtta tgtaaaaccg cttatt
//
LOCUS       DAZGQL010000094        18125 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.094, whole genome shotgun sequence.
ACCESSION   DAZGQL010000094 DAZGQL010000000
VERSION     DAZGQL010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18125
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.094"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..644)
                     /locus_tag="ACHKIR_003909"
     CDS             complement(<1..644)
                     /locus_tag="ACHKIR_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9991586.1"
                     /translation="MSIDFTPCMINTYHGDIYNRTTDTDNVKTPDTPKWPCDNWEEQQ
                     PINSTFSGEGYTSDQYDLAQYQLQQINACHTNTTYTNADYSKVVAQLVSLITNIETIS
                     STQLTQQTQSILNQINNIRYEKNKSAEYRIIVVANPKPDKAIITKISVEEGIPVRFSV
                     QTMFSDTNFITEQRADLPTNIKDIQSLYQKMTKLYIEHSENKNRMKVFAGTNFI"
     gene            complement(1225..2226)
                     /locus_tag="ACHKIR_003910"
     CDS             complement(1225..2226)
                     /locus_tag="ACHKIR_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFO9991587.1"
                     /translation="MSSWLAMLQDATEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGKGYLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLN
                     PIIVGLMWVTFGWKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCAPAK
                     TSPGTTYTPIKVSCCSPAAKAIEKPVVNCCSTKAVVSINPIKLATKDALQQFKDVLPY
                     LLLGVLIGSFIYGFIPSEWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMT
                     KGMGLGALMALIIGSAGASLTEVILLKSMFRIPMIVAFLTVILGMAILMGYLTQMLF"
     gene            2333..2629
                     /locus_tag="ACHKIR_003911"
     CDS             2333..2629
                     /locus_tag="ACHKIR_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFO9991588.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
     gene            complement(2752..3177)
                     /gene="arsC"
                     /locus_tag="ACHKIR_003912"
     CDS             complement(2752..3177)
                     /gene="arsC"
                     /locus_tag="ACHKIR_003912"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HFO9991589.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIVYYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQQGAFTKEDGEKVVDEAGKRLK"
     gene            complement(3190..4479)
                     /gene="arsB"
                     /locus_tag="ACHKIR_003913"
     CDS             complement(3190..4479)
                     /gene="arsB"
                     /locus_tag="ACHKIR_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HFO9991590.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVILHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLFLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTSKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLTALALRLSFTL"
     gene            complement(4534..4887)
                     /gene="arsR"
                     /locus_tag="ACHKIR_003914"
     CDS             complement(4534..4887)
                     /gene="arsR"
                     /locus_tag="ACHKIR_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HFO9991591.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLSLLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKVQVIVRN
                     LARQNCSADSKNICS"
     gene            complement(5135..5350)
                     /locus_tag="ACHKIR_003915"
     CDS             complement(5135..5350)
                     /locus_tag="ACHKIR_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000968019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9991592.1"
                     /translation="MMAISLQSEPPTQGWKVKNRRKVGFLHPDSDKAWYGSDPMKVYK
                     SSFTNMYSKLSLRQLKKSLTLAMLATG"
     gene            5629..5712
                     /gene="dinQ"
                     /locus_tag="ACHKIR_003916"
     CDS             5629..5712
                     /gene="dinQ"
                     /locus_tag="ACHKIR_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HFO9991593.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(5766..7118)
                     /gene="gorA"
                     /locus_tag="ACHKIR_003917"
     CDS             complement(5766..7118)
                     /gene="gorA"
                     /locus_tag="ACHKIR_003917"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFO9991594.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNVEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(7190..8032)
                     /gene="rlmJ"
                     /locus_tag="ACHKIR_003918"
     CDS             complement(7190..8032)
                     /gene="rlmJ"
                     /locus_tag="ACHKIR_003918"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HFO9991595.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLSSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATASW
                     IVPE"
     gene            8235..10277
                     /gene="prlC"
                     /locus_tag="ACHKIR_003919"
     CDS             8235..10277
                     /gene="prlC"
                     /locus_tag="ACHKIR_003919"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HFO9991596.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELAQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILIRGGSEEPMELFKRFRGREPQLDAMLEHYGIKG"
     gene            10285..11037
                     /gene="rsmJ"
                     /locus_tag="ACHKIR_003920"
     CDS             10285..11037
                     /gene="rsmJ"
                     /locus_tag="ACHKIR_003920"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HFO9991597.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(11086..12555)
                     /gene="dtpB"
                     /locus_tag="ACHKIR_003921"
     CDS             complement(11086..12555)
                     /gene="dtpB"
                     /locus_tag="ACHKIR_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HFO9991598.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGLSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFRPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            complement(12870..13304)
                     /gene="uspA"
                     /locus_tag="ACHKIR_003922"
     CDS             complement(12870..13304)
                     /gene="uspA"
                     /locus_tag="ACHKIR_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HFO9991599.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            13695..14030
                     /gene="uspB"
                     /locus_tag="ACHKIR_003923"
     CDS             13695..14030
                     /gene="uspB"
                     /locus_tag="ACHKIR_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HFO9991600.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(14101..15600)
                     /gene="pitA"
                     /locus_tag="ACHKIR_003924"
     CDS             complement(14101..15600)
                     /gene="pitA"
                     /locus_tag="ACHKIR_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HFO9991601.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            15832..17025
                     /gene="yhiN"
                     /locus_tag="ACHKIR_003925"
     CDS             15832..17025
                     /gene="yhiN"
                     /locus_tag="ACHKIR_003925"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFO9991602.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGITWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLELNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGIVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLVAE"
     gene            complement(17112..>18125)
                     /gene="yhiM"
                     /locus_tag="ACHKIR_003926"
     CDS             complement(17112..>18125)
                     /gene="yhiM"
                     /locus_tag="ACHKIR_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 family protein"
                     /protein_id="HFO9991603.1"
                     /translation="LLMGIICIALGEFVLTGSGQSEYFVAGHVLISLSAICLALFTTA
                     FIIISQLTHGMNKFYNRLFPVIGYAGSATTMIWGWSLLASNNVMADEFVAGHVIFGVG
                     MIAACVSTVAASSGHFLLIPKNASGSKSDGTPLQAYSSTIGNCLIAVPVLLTLFGFIW
                     SVILLRSADITPHYVAGHVLMGLTAICACLIGLVATIVHQTRNTFSVKEHWLWCYWVI
                     LLGSLTIIFGIYVLISSDASARLAPGIILICLGMICYSIFSKVWLLALVWRRTCSLAN
                     RIPMIPVFTCLFCLFLAAFLAEIAQTDMAYFIPSRVLVGLGAVCFTLFSIVSILEAGS
                     AKK"
BASE COUNT         4739 a         4489 c         4609 g         4288 t
ORIGIN      
        1 tcaataaagt tggttccagc aaagactttc atcctgtttt tattttcact gtgttcaata
       61 tataacttcg tcattttttg atacaggctt tgaatatctt ttatattagt cggtaagtca
      121 gctcgttgtt cagtaataaa attagtatct gaaaacatgg tttggacact gaatcttact
      181 ggtattccct cttctacgct aattttagtt atgattgcct tgtcgggttt aggattagcg
      241 acaacaatga ttcgatattc agcacttttg tttttttcgt atctaatgtt attaatctga
      301 ttaagtattg attgtgtctg ttgcgtaagt tgtgtcgaac ttatcgtttc aatgttagta
      361 ataagactta caagttgagc cacaactttt gagtagtctg catttgtata tgtagtgttc
      421 gtgtggcacg cattaatttg ttgaagttgg tattgcgcta aatcatattg atcagaggta
      481 tatccttctc cagagaatgt ggaattaatg ggttgttgtt cttcccaatt atcgcaaggc
      541 cacttcggtg tgtctggtgt tttaacattg tcggtatctg ttgtgcggtt ataaatatcg
      601 ccgtgatatg tatttatcat acatggggta aagtcaattg acataacatt ccttattgtt
      661 catctatttc gctaaatttt tgtaaatcgg agaattagag caggaatgga ttttcttcta
      721 cgcggaaata aacacaatgc gtcatatctt cctgatataa ttacctttaa cttccctttc
      781 atttccaccc ggtaatattt gtgtataaaa aaagggcagt gatagtatcg taaaggaaaa
      841 cgttaacaac tatcaaaaag cgctcatttt aaaaagttat gatgttcacc gatagtgtgt
      901 gaagtgatat ttttgtgatg ttttagatgt gtaataatga accaggttaa agtgtattta
      961 aaaatgtgtt gtattggcga ctgaaatctg ccctttaaaa ggggggatag cactgaaaca
     1021 aagagacatt gcttctccga taaaatgtca gagaagcatg attgcgtggt gattgtcgat
     1081 tgataactta ctgacggatc cgtcatcatt ctcaaagcat tagttaagtt atttgtgatt
     1141 tttaaggatt atcctatcta tgattttcta ctcagaagga taaggcactc acaatgctcc
     1201 ttatagttta ctgctggcat ctatttaaaa tagcatttga gtcaaatagc ccatcaagat
     1261 agccatacct aatataaccg tcaggaatgc aactatcatc ggtattctga acattgattt
     1321 aagtaatatc acttccgtca ggcttgcgcc ggcactgccg atgattaatg ccattaatgc
     1381 tcccagaccc attccttttg tcatcagaac agatgccaga gggataaccg cctctgcccg
     1441 gatatatagc ggaataccga caacggcgct caatgggatg gcgaagggat tatctgcccc
     1501 tgcatgagcg gcaatccact ctgagggaat aaagccataa ataaaagagc ctattaatac
     1561 ccctaacaaa agatatggca gtacatcttt aaactgttgc aacgcatctt tggtggctag
     1621 ttttatggga ttaatactta ccacagcttt ggtactgcaa cagtttacta caggtttctc
     1681 aatggcttta gccgctggac tacagcaact cacttttatt ggcgtatatg tcgtccccgg
     1741 cgaagttttg gctggagcac aacaatttgc tgatgagctt ttactggcaa tgatatgacg
     1801 ttcaaatccc agagaatcga ggataatact ggcaagtacg gagacgccgg cggcgataat
     1861 cgcatacaac aaggtaactt tccagccaaa ggtcacccac attaacccga cgataatggg
     1921 attaagtaat ggggaaacaa ataaaaaagt gagggtcgga ccaaacccgg ctttcgctga
     1981 taacaatcca cgtaacatag ggattgtcga gcaactacag aacggggtga cggctcccaa
     2041 cagagcagcc aggagatagc ctttcccttt tcttgccccc atcatctgct ggattttatg
     2101 gtctggcacc ttttgtctta tcaggctcac accggcacta atcactataa acagcaaaga
     2161 aagctcgacg gcgagaaaca caaacatctc tgtggcatct tgcagcatcg caagccaact
     2221 actcatacat aacctcattg aataaatcta tatttccaga ataatcgaaa tgaaattcag
     2281 atcaagatat ttctggtatt atcgaaatat caaccagaca gagggggatc atatgcagtt
     2341 ggaagaagtc gctaaagcgc tgaaagagct aggtcacccg acccgtttgt tcatatttaa
     2401 acatcttgtc aaagccgggg aacaagggtt gccggtcggt gagttgcaaa agcagcttgg
     2461 gatacccggc tctacactca gtcaccatat ttcagcactg gtatcggtag ggctggtcaa
     2521 acaaaaccgt gagagtcgaa cgctgatgtg tgtttcgcaa tacgcaatgc tggaagcgat
     2581 aattgaattc ttacgcgagg agtgctgcgt aaatagcaaa attgcctgat gcgctacgcg
     2641 ctacagattt catgccatgc acggttttgt aggtcggata agacgcgtta gcgtcgcatc
     2701 cgacataaac aaagcgcact ttaccaacta tgggcagtgg atatcgccgc tttatttcaa
     2761 gcgtttaccc gcttcatcaa ccactttctc gccatcttcc ttggtgaacg cgccttgttg
     2821 cgcatctggc agaatttcca gcacgacttc tgaagggcgg cacaggcgag ttcccaacgg
     2881 cgtcaccaca atcgggcgat taatcagaat cgggtgctga agcataaagt cgattaaccg
     2941 atcgtcagta aatttatctt ctgcaaggcc cagttcctca tacggctcga cgtttttacg
     3001 cagcagcgcg cgtacggaaa tccccatatc ggcaatgagt ttgaccagtt catcacgcgt
     3061 cggtggggtt tccagataat agacaatggt cggttctgtg ccgctgttgc ggatcatctc
     3121 cagcgtgtta cgcgacgtgc cgcaggccgg gttgtgataa atggtaatgt tgctcatatc
     3181 aatatctcat tacaaagtga aagagagacg tagcgccagc gcagtcagcg tcacaaacag
     3241 cacaggcagg gtcatgataa tccctgtacg gaagtaatat ccccagctga tagtcatatt
     3301 cttctgcgaa agtacgtgca gccagagcag cgtcgccagg ctaccaatcg gggtaatttt
     3361 cggccccaaa tcgcagccaa ttacgttggc ataaaccatc gcttctttga taacgccaga
     3421 tgccgtgctg ccatcaatgg acaacgcgcc aaccagtacc gtcggcatat tgttcataat
     3481 agaagagagg aaggcggtga ggaatccggt gccgagcgtc gcggcccaca ggccattatc
     3541 cgccagcacg ttgagtacgc cagaaagata ttccgttagt ccggcattgc gcaggccata
     3601 aaccaccaga tacatgccga gcgagaagat gacaatctgc cagggcgcac cgcgtagcac
     3661 cttactcgta ttaatcgcat gaccgcgttt agcgacgaca aataatatca acgcgcccac
     3721 tgccgcaatg gcgctgaccg gaatgccgag cggttccagg acgaaaaatc ccaccagcag
     3781 aaacagtaaa acaacccagc cagttttgaa ggtggcagga tctttaatcg cttctgcggg
     3841 agatttcagc agcgccatat cgtagttctg cggaatatct ttgcggaaat agagatgcaa
     3901 aatcaccagc gtggcaacaa ttgcggcgat atccaccggc accatcaccg atgcgtattc
     3961 gcgaaagccg aggccaaaga aatctgcgga aacgatattc accaggttgg agacaataag
     4021 cggcaggctg gcggtatcgg caatgaatcc ggccgccatc acgaacgcca gcgtagtgcc
     4081 tttactgaac cctaaagcca gcaacatggc gatgacaatc ggtgtcaaaa taagcgccgc
     4141 gccatcattg gcaaacagag ctgcaacggc agcaccgagc aggacaatcc aggtgaacag
     4201 caagcgaccg cgtccattgc cccagcgtga gacgtgcagc gccgcccatt caaaaaagcc
     4261 ggactcatcc agcagcaggc tgatgataat gacggcaata aacgccgccg tcgcgttcca
     4321 gacgatattc cacaccaccg gaatatcacc cggatggacc acgcccgtaa ctaacgccag
     4381 tactgcgccg agcgttgcac tccagccgat gcctaaacct ttcggctgcc agataaccaa
     4441 tacaatggtc aggacaaaga tagcgcctgc cagtaacata atgcctcccg gataaaacac
     4501 atgcgaaaat acatatgtgt ttagctaaat tttttaactg caaatgttct tactgtctgc
     4561 ggaacaattt tgtcgagcca ggttgcggac aatcacctga accttttcct gttcacatcg
     4621 ccaggcctca tcaataattt tcgccgccca tgctggaata tgcggtgata agcggtaatg
     4681 aacccactta ccttgcttgc gatccagcaa tagcccactt tcacgcagca atgacagatg
     4741 gcgggatatc ttgggctgcg actgatcgag ggcagtgcag agatcgcaga cgcataactc
     4801 tcccagttcg ctaagcagta aaacgatgcc cagacgggtc tcatcagcaa gaattttgaa
     4861 cagttgaatg ggtaacagaa atgacatatt gcgctcctga ttgttgcagg tagtgtctct
     4921 cttcgaagcg gataagtcaa aaacatatat ggtttaacga atgtgtaagt gcagaggaag
     4981 gtaataggtg tgaattttga gttgggtatt catttgaaag gaggtctgaa tcaggacgta
     5041 aaaaaaccga ctttgcgtcg gttttttact ttccagccct gagttggtgg ctctggctgg
     5101 agtgagaatg cccacatcta acggttcaat actattagcc agtggctaac attgcaagcg
     5161 ttagcgattt cttcagctgg cggagggaca gtttgctata catgttggtg aaagaggatt
     5221 tatatacttt catcgggtcg ctgccgtacc aggccttgtc cgaatccgga tgtaaaaaac
     5281 cgactttgcg tcggtttttt actttccagc cctgagttgg tggctctgac tggagtgaga
     5341 tagccatcat ctaacgcatc aatgctaata ccatactgaa actattgcaa ggacatgctg
     5401 gttttataac ccgcaagtac tgtatgatta tccagttagc tctgagacat tttcactctg
     5461 gcaatgcgca taaacgcttt caaagtcctg gtcagaagta cgggtggtgc cgttaactga
     5521 tgctctggcc ggagtgagaa tgcccacatc taacaatggg gcatgcgccg tgacaggcag
     5581 tggatgagta agcggatgca ttctcactcc atcgcatgga gaaaacgggt gattgataaa
     5641 gcaatcatcg ttcttggggc gttaattgcg ctgctggaac tgatccgctt tctgcttcag
     5701 cttctgaact gatagcggaa acgtagctaa gggctaagag cacactactc ttagcccttt
     5761 aacatttaac gcattgtcac gaactcttct gccgctgtcg ggtgaatggc aaccgtgttg
     5821 tcgaagtctt ttttggttgc gcccatcttc agcgccaccg cgaagccctg caacatttca
     5881 tccataccaa aaccaatgcc gtgaattccg acaatcttct cttccggtcc aacgcatacc
     5941 agtttcatgc ggcacggctg gcggtgagtg gtgacggcgg tatacatcgc ggtgaaagag
     6001 gatttataca ctttcacctg atcgtcgccg tactgctcgc gcgcctgcgg ttccgttaaa
     6061 ccaacagtcc caatcggcgg atggctgaag accacggtcg gaatgttgct gtaatccaga
     6121 tgctcatccg gcttgttatt aaacaggcgt tcagagagac ggcgacccgc tgcaactgcc
     6181 accggtgtca gttccactgc acccgtgtta tcgcccaccg cgtaaatacc ttcaacgttg
     6241 gtattttgat atttatcgac gacgatatag cctttttcgt tagttttaac gccagcggct
     6301 tccaggttga tgttgtcatt ggcaggctcg cgaccaatcg cccaaatcag gcaatccacc
     6361 gtttcactgc gcccatcttc cagctccagc gtcaggctac catcagcatt tttcactact
     6421 gctttcggga tggcgttggt atgcaactgc gggccttcgg cgttcatcac ttcgaccagc
     6481 gtttcagaga tcatcgggtc gaagctgcgc agcggcgcat gtttacgcac aaacagatgc
     6541 gttttcgcgc cgaggccgtt aatcacgccc gccagttcca cggcgatgta acctgcgcca
     6601 acaaccgcca cgcgctctgg caaagcagga agggcgaaga agccatcaga atcaataccg
     6661 tattccacgc ctggaatatc cgggtggctc ggacgaccgc cggtggcgat cagaatatga
     6721 tcggccgtga tggtttcgcc gtttacctcc agcgttttgg catcaacgaa gcgggcaaag
     6781 cctttgatta catcaacgtt atttttaccg agcacgtttt cataggaagt atgaatacgg
     6841 tcgatatagg cggtacggct ggcgatcaac gtttcccagt tgaatttatt gatagtggta
     6901 tcgaaaccat aatccggacc gtacatgtgg atcgcttcac ggatttgcgc cgcgtgccac
     6961 atcacttttt tcggcacgca gccgacgttc acgcaggtgc cgcccagttc tttggcttca
     7021 atcagcgcgc atttctgacc gtacatagcc gcgcggttga tggaggcgat accgccgctg
     7081 ccgccgccga tggcgatgta atcatagtgt ttagtcatga catattgtcc ttatcgttga
     7141 ttaccgcgat tgtagcgcga gacgtaatag gtgccagcga tggctgcaat tactccggca
     7201 cgatccagct tgcggtggcg tgcccagtgc cagctggaac cagtttactg tgcagccacg
     7261 gcagcacgtt attcatctgc tgttccagct tccacggcgg attaatcaca atcatgccgg
     7321 aagcggtcat gccacggcga tcgctgtctg gcagcaccgc cagttcaatt tgcagaattt
     7381 tgcgaatgcc ggtcgcttcc aggtcgtgga tcatgcgctt aatttgctgg cgcagcacca
     7441 ctggatacca caatgcgtaa gtgccagtgg cgaaacgttt gtaaccttct gctatcccgc
     7501 tgaccaccgc ctgatagtcg gttttcattt catacggcgg gtcgataagg attaaaccac
     7561 ggcgggaaac cggcggcagc ttggccttaa gctgctggaa accgtcggct ttttcgacgc
     7621 gcgcacggct gtctttctga aattcagaac gcagcaacgg gtaatcgctc gggtgcagct
     7681 cggtcagttg caggctgtcc tgttcacgca gtagctggcg agcaatcaac ggcgaaccgg
     7741 ggtaataacg caactgcccg ctacggttga agtgttttac cacattgatg tacgcctcca
     7801 gctccgcagg caaatcgtcc tgctgccaga tacgggcgat accttcgaga tattcgccgg
     7861 tacgctcggc atgttcgctg cttaactgat aacgcccggc ccctgcgtgg gtgtcgagat
     7921 agagaaacgg tttatctttc tctttcagcg actcgatgat caggctctga acggtatgtt
     7981 taaggacgtc ggcgtggttg ccagcgtgaa agctgtggcg ataactgagc atgggtaaag
     8041 gtgttccggt aagtaaaaat cggcccgtat tcggggcgca caaaagccta tcaggacagt
     8101 ataccgaaaa gaggccgccg ccgcgaaagc gtaacgtttc tcattgaaat tcactacact
     8161 taaccccatg ctacacacat tatgtaaagc gcctgttgag cgcttcctta acctctttaa
     8221 ccaggactgc gctaatgacg aatccgttac tgactccctt tgaattgcct ccgttttcta
     8281 aaattctccc ggaacatgtc gttccagccg tgactaaggc gctgaatgac tgccgcgaaa
     8341 acgtggagcg cgtagtagcg caaggggcac cgtacacctg ggaaaatctc tgccagccgt
     8401 tggcagaagt agacgatgtg ctggggcgta tcttctcccc ggtcagccac ctgaactcgg
     8461 tgaaaaatag cccggaactg cgtgaagcgt acgaacaaac cctgccgctg ctgtcagaat
     8521 acagcacctg ggtagggcaa catgaagggc tgtataaagc gtatcgcgac ctgcgcgatg
     8581 gcgatcatta cgccacgctg aacacggcgc agaaaaaagc ggttgataac gcactgcgcg
     8641 acttcgaact ctctggcatc ggtctgccaa aagagaaaca acaacgctac ggtgagattg
     8701 ccacccgtct ttccgagctg ggcaaccagt acagcaacaa cgtcctcgat gcgacaatgg
     8761 gctggaccaa actcgttacc gacgaagcgg aactggcagg aatgccagaa agcgcactgg
     8821 ctgcggcaaa agcccaggct gaagcgaaag agctggaagg ttatttgctg acgctggata
     8881 tcccaagcta cttgccggta atgacttact gcgacaacca ggccttgcgt gaagagatgt
     8941 atcgtgctta cagcacccgc gcttccgatc aaggcccgaa cgccggtaag tgggacaaca
     9001 gcaaggtgat ggaagagatc ctcgcgctgc gtcacgaact ggcgcaactg ctgggttttg
     9061 aaaactacgc ctttaaatcc cttgccacta aaatggcaga aaatccgcag caggtgcttg
     9121 atttcttaac cgatctggca aaacgcgcgc gtccgcaagg tgaaaaagag ctggcgcaac
     9181 tgcgtgcctt cgctaaagcc gaatttggcg tcgatgagtt gcagccgtgg gatatcgctt
     9241 actacagcga aaaacaaaaa cagcatctct acagcatcag tgacgaacag ctgcgtccgt
     9301 acttcccgga aaacaaagcg gttaacggct tgtttgaagt ggtgaaacgt atttacggca
     9361 tcaccgctaa agagcgtaaa gatgttgatg tctggcatcc ggatgtacgt ttcttcgaac
     9421 tgtatgacga gaacaacgaa ctgcgcggca gcttctacct cgacctgtat gcccgtgaaa
     9481 acaagcgcgg cggggcgtgg atggatgact gcgtaggcca gatgcgtaaa gccgacggtt
     9541 cgctgcaaaa accggtcgcg tatctgacct gtaacttcaa ccgcccggta aatggtaaac
     9601 cggcactgtt tacccatgac gaagtgatca ccctgttcca tgaattcggt cacggcctgc
     9661 accatatgct gacccgcatt gaaactgctg gtgtatctgg tatcagcggg gtgccgtggg
     9721 atgcggtcga actgccgagt cagtttatgg aaaactggtg ctgggagccg gaggcgctgg
     9781 cgtttatctc cggtcactat gaaaccggcg aaccgctgcc gaaggagctg ctggataaaa
     9841 tgctggcggc taagaactac caggcggcgc tgtttattct gcgccaactg gagttcggcc
     9901 tgtttgattt ccgcctccat gccgagttcc gcccggatca gggagcgaaa atcctcgaaa
     9961 ctctggcaga aatcaagaaa ctggttgccg tagtaccgtc tccatcctgg ggccgtttcc
    10021 cgcacgcttt cagccatatt ttcgccggtg gttatgccgc aggttactac agctacctgt
    10081 gggccgacgt gctggcggca gatgccttct cgcgttttga ggaagagggc attttcaacc
    10141 gtgaaactgg gcagtcgttc ctcgacaaca ttctgatccg tggcggttca gaagagccga
    10201 tggagctgtt caaacgcttc cgtggtcgtg aaccacagct ggatgcgatg ctggagcatt
    10261 acggcattaa gggctgaaca ttcagtgaaa atctgcttaa ttgatgaaac aggcgccgga
    10321 gacggcgcct tatctgttct ggcggcccgc tgggggctgg agcacgatga agacaacctg
    10381 atggcgctgg tgttaacacc ggagcacctg gaactgcgca agcgtgatga gccgaagctg
    10441 ggcggcatct ttgttgattt tgttggcggg gcgatggcgc accgacgcaa attcggcggt
    10501 ggtcgcggag aggcggtggc gaaagcggtg ggcattaaag gcgattattt gccggatgtg
    10561 gtggacgcca ccgcagggct ggggcgcgat gcctttgtgc tggcttcggt cggctgccgc
    10621 gtgcggatgc tggagcgtaa tccggtggtt gctgcattgc tcgacgacgg cctggcgcgt
    10681 ggttatgcgg atgcggaaat cggcggctgg ttgcaggagc ggttgcagtt aattcacgct
    10741 tccagcctga cggcgctgac cgatattact ccgcgcccgc aggtggtcta tctcgatccg
    10801 atgttcccgc ataagcagaa aagcgcgttg gtgaaaaaag agatgcgtgt ctttcagtcg
    10861 ctggtggggc cggatcttga tgccgatgga ttgctggagc ctgcacgctt gctggcgacc
    10921 aaacgcgtgg tggtaaagcg cccggactac gcgccgccgc tggcgaatgt cgccacgcca
    10981 aacgcggtag tgaccaaagg gcatcgcttt gatatttatg ccggaacgcc ggtgtaaaag
    11041 taaagcaggg tagcgttatc gctaccctgc tttctgcaag aataattaat ggctttcggg
    11101 cgtcgcaatc atgcgtttca gccacggcac catcagcagc atcaccaccg caacgcccag
    11161 cgtaaccagg ccaatcttac caaacacgtt ggtatagacg ggcaacgtct caagcggatc
    11221 ggtaatgttg tctggtactg cggtaaatgt tgccacatag ccgcccagca agaacgcggc
    11281 agcctgcgtc aggaaccaca tcccgagaat aaagcccatc aaatgctgcg gcaccagggc
    11341 agcaatcatc gccagaccca gggcgctaat aaacaattca cccaggctct ggaacaagta
    11401 aaccagcacg ataaaccacg gcgatgtcag tccttgtgca tcagcaaacc acattcccgc
    11461 agcggcggct gtaagaaagc ccagcgagca cataaacatg ccgagagtaa atttcatcgg
    11521 catcgagagg tctttgcctt tgttacccag atgcgtgtaa atgcctgcca gtattgggct
    11581 ggcgagtacc acccagaacg ggttaagcgc ctggaagctg actgggttga tggaaaagcc
    11641 gagaatttca tgatgcacgt tgttgatagc aaagaagttc agcgatgttg gcatctgggc
    11701 gtagagaatg taaaacacca ccgcttcgag catcaggaca aaggcgacaa acattttatt
    11761 gcgcccggtt ttatccagct tgaatgcctg acgaaagaaa atgatggtga cgacgatgga
    11821 gagaacaatc agcaccagat tggcgacttc tacgttgtgc atcagccatg cacagacgaa
    11881 gatcatcacc acgctgccaa gtaacacgta caacagtttg ctgaagctca tcggccggaa
    11941 gtcgggttca gaaccaatgt ctttcaccat tccgcgacag gcgatgtaaa ccaatagcgc
    12001 gataattaat cccgcaccgc acaggttgta ggtgactgaa tagccgaatc tatcagcgat
    12061 cacaggggcc agcgataacg ctatcaacga gccgatgttg atcgacatat agaacagggt
    12121 aaatgcgcca tcaagccgcg gatctttcgg cggatagcac ttcgaaagca agctggctgg
    12181 gttagcttta aacaggccgt taccgacagc gatagtcccc agggcgatga aaatcaggtc
    12241 aggcttaagt agcgacaggc cggtcatgaa gtagccaatc gccagcacaa gtgctccgag
    12301 gacaatggtg cgtttggtcc ccagcaggtg gtcgccgaca tagccgccaa tggaaatgag
    12361 gccatagacc agcgcagcaa aagcaccaaa agtgacaaaa gcctgttctt gcgagaatcc
    12421 aagctgctta acgaagaaaa ccgccagtac gccctgcacg ccgtagtagc cgaatcgctc
    12481 ccataactcg acaaaaaaga tcatgaaaaa tgggcgaggt tgctgcagca tccccatggg
    12541 tgttgttgta ttcatattta ttaaccttcc aataccatcc tgaaaagtat gaacgcgtaa
    12601 gggataacgc gcgaaaaagt ttacatctgt cagagattct taatattgat gcggtgaata
    12661 gattaataca ctattacaac agaaaataac cagctgatca cactaatgat ggaaaatatg
    12721 atggtgttaa atgttgtatt ggcaggggat tttgattgcg tacgcagacc gtggccagat
    12781 aaggtgttta cgctgcaagg taacaaggcg cgccaaaaag caaaacgccc gcatatgcgg
    12841 gcgttcagga acacgtcgta gagggaagat tattcttctt cgtcgcgcag cggaacaatc
    12901 agcatatcaa cgtgaacggt gttgatcagc tgacgtgcgg aagacatcag tttgctccag
    12961 aagtcctggt ggtgaccaca aaccaccaaa tccatatcgt atttcttgat tgcatcgacc
    13021 agaacctggc ccaggtcgcc gctgccgctc agggtttcag tgattgggta gcctgcatta
    13081 gtggaaagct cggtcagcgc atgatgtgtc tcttcagaga tgcgtttctg catatcaccc
    13141 agattcacat caataagccc ggtgtatagg tcagagtagt ttacatctac gtggatcaga
    13201 gaaactttcg cattgtaggg gcgagccata gagactgctt tctctaccag aactttgctt
    13261 tccggggaga gatcgaccgc gatgagaatg tgtttataag ccatagtgtt actccttcca
    13321 taaagttgtc gatgactggc cagctagcgt ttcttgtgcc gttacgtcac ccgcgtcctg
    13381 cgaactacgt gcgcggggct aacccagccg taaagattca gtgttaagac catccttacc
    13441 ttatagcgac ccggatgatc cgtcaatccg ccttgcttac ccgttagata aacaaatttt
    13501 cgacatgaat atattgatag tggttaacct tctggaaaaa aaacaacctg atctcctaca
    13561 ctatctatag agccgctcgg atgttggtca ccacaaaagc ggtttctcca ggacctggtg
    13621 ttaactgatt ggcagcgtat cgggagcggt agccccgggg tggaactccg cgggcaggtc
    13681 gccggggagg aggtatgata agcaccgtcg cattattttg ggctttatgt gtcgtttgca
    13741 ttgttaacat ggcgcgctat ttctcatcac tacgcgcgtt gttagtggta ctgcgtaact
    13801 gcgatccatt gctctatcaa tatgttgatg gagggggctt ttttacctca catggccaac
    13861 ccaacaaaca ggtgcgtctc gtttggtata tctatgccca acgttatcgc gatcatcacg
    13921 atgatgagtt tattcgccgc tgtgagcggg tgcgtcggca gtttattctg accagcgcat
    13981 tgtgtggtct ggtggtggtc agtctgattg cattgatgat ttggcattaa gcacttcagc
    14041 aaaaataacg cataaaaaaa gcgggccagt aaactgaccc gctcgttttg gtgcgtgcga
    14101 ttacaggaac tgcaaggaga gccagtacag cccgccggaa agcagtaccg cagccggaag
    14161 ggtaaacacc caggccatca gaatactggt cacggtttta cgctgtaagc cgccgccatc
    14221 taccaccatc gtccccgcga cagaagagga gagtacgtgg gtagtggaaa ccggcatccc
    14281 ggtataactc gccaggccga tagacactgc cgcagtcatc tgggcagaca ttccctgagc
    14341 gtaggtcatg cctttcttac cgattttctc accgatagtc gttgccacac gacgccagcc
    14401 aatcatcgta ccgataccta acgccagcgc gaccgccatg atgatccaca ccggtgcata
    14461 ctcgatggtg ctaagcatgt cagacttcag tttcttcaac aggcgctgat cgtcagcact
    14521 cacgccaggc atcttcacca ctttgtcgat agtgtcagaa acgcacagca taatgcggcg
    14581 catctggcta cgttgatcaa gcgacagctt atcgtagctt tccacatcgg tagtcaacat
    14641 gcctttcagg cggttgagcg cgttaatggt attcgacgga tggcaatgga actccgcagg
    14701 ttgcgttgcg ccagcttccg gagccggtac taactgatca gcaccggtcg cttgtttcag
    14761 cagcgcagga tgctgctcaa agtaagcttc gacgttgttg atggcatcac gggtacgggt
    14821 gatttcgtag ccagtggcat tcatgttcac cacgaagcct gctggtgcga caccaatcaa
    14881 taccaacata accagaccaa tgcctttctg accatcgttc gcgccatgcg aaaacgccac
    14941 gccgatagcg gaaaggatca gcgcaatacg cgtccagaac ggcggctttt tcttgccgtc
    15001 tttcttttca cgctccgctg gggtcaggtg gatacgggcg cgtttcttgg tgccgctcca
    15061 gtagcgacgc agcaagaaaa tcagaccgcc agcaaacacc aggccgacaa taggggaaac
    15121 gatcagagaa ccgaaaatac ttaatacttt cgggatattg agtgcatcca ccactgacgt
    15181 cccggtcatc aacgcattgg ttaaaccaat cccgatgatc gcgccaatca gcgtatgaga
    15241 gctggatgca ggtaaaccaa agtaccaggt acccaggttc cagataatcg ccgccagcaa
    15301 catagagaac accatggcaa ggccatgaga cgatcccata ttaagcagca gatccgtcgg
    15361 cagcatatgc acaatggcat aggcaacact cagaccaccc agcaaaacac ccaaaaagtt
    15421 gaacaccgcc gccataacca cggcgagctg agaacgcatc gcgcgggtat agataacggt
    15481 tgccacggcg ttggctgtgt catggaaacc attgatggct tcgtagaaca gcacaaaagc
    15541 cagtgcaagc aataataaca gcccggtatg caaatccagg ccagcaaaca aatgtagcat
    15601 aggacgttac gccattttga ggacatgaac gcggcgcatt atcagtgact ttcgcggcgc
    15661 gggcaaagtg aaatatagac tttttttgat ttgcctcctg tatggatttc actcaaaaaa
    15721 taattatctt atataattca ggtaaatact tccttttagt aatattgatg ctggtgcgac
    15781 cactgaggaa tctttacaat tcacgcccgt tttttcttag aggagcgcaa agtggaaagg
    15841 tttgatgcca ttattatagg cgctggtgcg gcgggtatgt tctgttctgc gctggcaggt
    15901 caggcaggac gccgggttct gctgatcgat aatggtaaaa aaccagggcg caaaatcctc
    15961 atgtctggcg gtgggcgctg caactttacc aacctttatg tcgaaccggg cgcttatctg
    16021 agccagaatc cgcatttttg taagtctgca ctcgcgcgtt ttacccagtg ggatttcatt
    16081 gatctggtca ataaacacgg catcacctgg cacgagaaaa cgttagggca actcttctgc
    16141 gatgactccg cgcagcagat tgtcgacatg ctggtggatg agtgcgagaa gggcaatgtg
    16201 accttcagat tgcgtagcga agtgctgagt gtggcgaagg atgaaacagg cttcacgctt
    16261 gaactgaacg gcatgactgt cggttgcgaa aagctggtca tcgcgaccgg tggactgtca
    16321 atgccagggc tgggcgcgtc gccgtttggt tataagattg ccgaacaatt tggcctcaac
    16381 gtgctgccga cccgcgcggg cctggtgcca ttcactctgc ataaaccgtt gctcgaagag
    16441 ttacaggtgc tggcgggcgt ggcggtgcct tccgtgatta ccgctgaaaa cggcatcgtt
    16501 ttccgtgaga acttactctt cacccaccgc ggcttatctg gaccggcggt gttgcaaatt
    16561 tccagctact ggcaaccggg tgaatttgtc agtatcaatc tgctaccgga tgtggacctc
    16621 gaaaccttcc tgaatgagca gcgtaatgca catccgaatc agagcctgaa aaacacactg
    16681 gcggttcatc taccgaagcg gttggttgaa cgtttacaac aactcgggca aatcccggat
    16741 gtttcgctaa aacagctcaa cgtgcgtgac caacaggcac tgattagcac attgaccgac
    16801 tggcgcgtac aacccaacgg cactgaaggc tatcgcactg ccgaagtgac gctcggcggc
    16861 gtggacacca acgaactctc ttcacggacg atggaagcgc gcaaagtgcc tgggctgtac
    16921 ttcatcggcg aagtgatgga cgtcaccggc tggctggggg gctataactt ccagtgggcg
    16981 tggtcgagtg catgggcttg tgcgcaggat ttggtggcag aataagaagc aaaatgtgct
    17041 ctcgatacca gagggattaa cctgaataaa accagataag gcgtccaacg ccttatctga
    17101 agcctttttt attatttttt cgctgaaccc gcttctaata tagaaacgat ggaaaacagt
    17161 gtaaaacaaa ctgcacctaa gccaaccaga actcgcgaag gaataaagta agccatatcg
    17221 gtttgcgcaa tttcagccag gaacgcagca aggaacagac aaaacagaca ggtaaatacc
    17281 ggaatcatgg gtattctgtt ggctaacgaa caggtgcgtc gccataccag cgctaacaac
    17341 cacactttgg agaaaatact gtagcagatc atccccaggc aaatgagaat aatgccgggg
    17401 gccaggcggg cgcttgcatc tgaactgatt aatacgtaga taccaaaaat aatggtaagt
    17461 gagccaagca aaataaccca atagcaccac agccaatgtt ctttcactga gaacgtattc
    17521 cgcgtttgat ggacaatagt ggcgacaagg ccaataagac aagcgcaaat tgccgttaac
    17581 cccattagta cgtgacctgc aacataatgt ggtgtgatat ctgcactacg taacagtatg
    17641 actgaccaaa tgaaaccaaa gagagtaagc agaacgggaa cggcaattaa gcaattaccg
    17701 attgttgaag aataggcctg caatggcgta ccgtcactct tactgcccga ggcgtttttg
    17761 gggatcaaca gaaaatgtcc ggaagatgcc gctacggttg atacacaagc ggcaatcatg
    17821 ccaacgccga aaataacatg accggcgaca aattcatctg ccatcacatt attgcttgcc
    17881 aacagtgacc atccccaaat catagttgta gcagaaccgg cataaccaat aacgggaaat
    17941 aacctgttgt agaatttatt catgccgtga gttaactgag atataataat aaatgcggta
    18001 gtgaacaatg ccagacatat cgcagaaaga gaaatcagaa catgccctgc aacgaaatat
    18061 tcactttgcc cagaaccggt taatacaaac tcacctaatg cgatgcatat tatacccatc
    18121 agtaa
//
LOCUS       DAZGQL010000095        18085 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.095, whole genome shotgun sequence.
ACCESSION   DAZGQL010000095 DAZGQL010000000
VERSION     DAZGQL010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18085
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.095"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(239..1084)
                     /gene="sseA"
                     /locus_tag="ACHKIR_003927"
     CDS             complement(239..1084)
                     /gene="sseA"
                     /locus_tag="ACHKIR_003927"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HFO9991604.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPEK"
     gene            1291..6252
                     /gene="yfhM"
                     /locus_tag="ACHKIR_003928"
     CDS             1291..6252
                     /gene="yfhM"
                     /locus_tag="ACHKIR_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HFO9991605.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     VSSAKLSAPERQKLAQQSAGKALTLLDLSEVQLDGAATLVLTFSIPLDPDQEFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVIEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNSARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGVEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPDQPIKLDVIKPDGQVLRSVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPKDEVRFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGIRPQFASK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLKADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVPAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPTKMRPNQPLTVKIKASNKNGEMPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LPVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGDIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAL
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTTSGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFTLWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGVALKTMGDAMRGEEAIVLALKTPRNSDERIW
                     LGDYGSPLRDSALMLSLLEENKLLPDEQYSLLNTLSQQAFGERWLSTQESNALFLAAR
                     TLQDLPGKWQAQTTFSAEPLTGEKAQTSNLNSDQLATLQVTNSGDQPLWLRVDASGYP
                     QSAPLSASNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     AGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAADDLLIVRP"
     gene            6253..8565
                     /gene="pbpC"
                     /locus_tag="ACHKIR_003929"
     CDS             6253..8565
                     /gene="pbpC"
                     /locus_tag="ACHKIR_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HFO9991606.1"
                     /translation="MPRLLTKRGCWIMLAAAPFIIILAAWAADKLWPLPLQEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWMHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAVQGVWSRERVKESREEPIWLAPRQMPQLALLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAQPLPDGALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAIPLLNQVNNILLS
                     RSVNLPEDPRPDSVSRGVICWPGGQSLAEGDGNCRRRLATWLLDGSQPPTLLLPEQEG
                     INGIRFPIWLDENGKRVAADCPQARQEMINVWPLPLEPWLPASERRAVRLPPASTICP
                     PYGHDAQLPLQLTGARDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDEVGQIATVKFVMQ"
     gene            8714..9145
                     /gene="ndk"
                     /locus_tag="ACHKIR_003930"
     CDS             8714..9145
                     /gene="ndk"
                     /locus_tag="ACHKIR_003930"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFO9991607.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGSIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            9295..10449
                     /locus_tag="ACHKIR_003931"
     CDS             9295..10449
                     /locus_tag="ACHKIR_003931"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HFO9991608.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            10734..11741
                     /gene="rodZ"
                     /locus_tag="ACHKIR_003932"
     CDS             10734..11741
                     /gene="rodZ"
                     /locus_tag="ACHKIR_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HFO9991609.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELNNNQSQ
                     SVPLDTSTTTDQAMATTPTSPVDTTATNTQTPAVTAPAPAVDPQQNAVVPPSQANVDT
                     AATPAPAATTTPDGAAPLPTDQAGVTTPAVDPNALVMNFTADCWLEVTDATGKKLFSG
                     MQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQSPA
                     Q"
     gene            11768..12886
                     /gene="ispG"
                     /locus_tag="ACHKIR_003933"
                     /gene_synonym="gcpE"
     CDS             11768..12886
                     /gene="ispG"
                     /locus_tag="ACHKIR_003933"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HFO9991610.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQQVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            12997..14271
                     /gene="hisS"
                     /locus_tag="ACHKIR_003934"
     CDS             12997..14271
                     /gene="hisS"
                     /locus_tag="ACHKIR_003934"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFO9991611.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRTLGISEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQVL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            14289..14909
                     /gene="yfgM"
                     /locus_tag="ACHKIR_003935"
     CDS             14289..14909
                     /gene="yfgM"
                     /locus_tag="ACHKIR_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HFO9991612.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDAIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            14920..16098
                     /gene="bamB"
                     /locus_tag="ACHKIR_003936"
     CDS             14920..16098
                     /gene="bamB"
                     /locus_tag="ACHKIR_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HFO9991613.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVNLAEKDG
                     WFSKDPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            16216..17688
                     /gene="der"
                     /locus_tag="ACHKIR_003937"
     CDS             16216..17688
                     /gene="der"
                     /locus_tag="ACHKIR_003937"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFO9991614.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYALGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEADDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKSK"
     gene            17751..17966
                     /locus_tag="ACHKIR_003938"
     CDS             17751..17966
                     /locus_tag="ACHKIR_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFO9991615.1"
                     /translation="MALNCPTCKTPLERNGETAHCATCDKDFTVQALCPECHQALQVL
                     KACGAVDYFCQHGHGLISKKRVEFIIA"
BASE COUNT         4351 a         4439 c         5052 g         4243 t
ORIGIN      
        1 aataagccag gttattttat taccgaacac ggcggaaagg gccggtgtcg ataatacgct
       61 aaataaacaa tgattactct ctttcgcttg acaaaaagag agttactggc gagtattgct
      121 ttgctgtgaa gcacctgcgt tgctcatgca acacaagcca atctgctgga aaggccgcga
      181 accagaccag caataaaaaa ccgccagatt cggcggtttt ttattgcctg ttaaaagctt
      241 atttctctgg ctcaactggt aaatctgctc gtgcgcccca ttcactccat gcgccgtcgt
      301 acagtttcac gtttggcaca tcaagtgtcg cgagtgccaa caaaaccacg gctgccgtta
      361 caccagagcc gcagctgacg ataataggtt tgtcataact tacgccgcga ccaaaaaata
      421 tcgcatccag ttcatcggtc gtttttagtt caccttcgcg caccagttcc gtccacggaa
      481 catttagtgc accgggaata tgtccgcgac gtaaacctgg gcgaggttca tcaacttctg
      541 cgttaaaacg tgcagccggg cgggcatcga taatttgcgc cgtattttca tggcttgcca
      601 ataacacatc ggttactttc accacggctt caggattaaa cgcggcgtta aactctcctt
      661 ccggcagctc tactgcgcct tcttctaata gcagatcatc acgctgccag cctgcaagtc
      721 caccacccag aatcgacact ttctctacac caaaggtgcg cagcatccac catgctcgtg
      781 gggctgagaa aagattacct tcgtcataga caatcaggtg cttatcctgg ttaacgccta
      841 attcacgcat cgccacggcg aacgtttccg ggcgcggcag catgtgcgga agcggggaag
      901 tgtgatcaga aagcgcttct atatcaaaaa acactgcgcc gggaatatgt ccattcagat
      961 actcctgagc aacgttacga tcttcctgtc caggcgacgc catgcgggca tcgataatct
     1021 gaatttccgg gtcatcaata tgttcggcga gccagtcggc tcctacaaac catgtcgtgg
     1081 acataggcat ctccattttt gccggggtta ggctaaattg tcggatgttt agtgaaaggc
     1141 gacaagcaaa agaggctgat ttggctgtct gagccacaat tctcactaaa attcggattg
     1201 tatgggcgtg atactgaatt acataacgta aattctcacg cataataatt attcgacgtt
     1261 gctaacgggc cattgtggca gggatgaaaa atgaaaaagt tacgcgtagc cgcctgcatg
     1321 ctaatgctgg cgctggcagg gtgcgacaac aacgataacg cgccaacagc ggtgaaaaaa
     1381 gatgcgcctt ctgaagttac taaagcggcc tcttcagaaa acgtgagttc agcaaaactt
     1441 tccgcgccag agcgacaaaa actggcccaa cagagtgccg gtaaggcgct gacattgctg
     1501 gatctctctg aagttcaact tgatggcgca gccacgctgg tgctgacatt ctccatccct
     1561 ctcgacccgg atcaggaatt ctcacgcgtt atccatgtcg tcgataagaa aagcggcaaa
     1621 gtggatggtg cctgggagct gtcagataat cttaaagagt tgcgtttacg ccacctcgaa
     1681 ccgaaacgtg atttgatcgt cactattggc aaggaggtca aagcactcaa caacgcaacc
     1741 ttcagtaaag attacgaaaa aactataact acccgagata tccaacccag cgtcggtttt
     1801 gccagccgtg gctcgttgtt acctggtaaa gttattgaag ggctgccggt aatggcgctc
     1861 aacgttaaca atgtcgatgt taacttcttc cgtgttaagc cagaatctct gcctgcattc
     1921 attagccaat gggaataccg caattcgctg gcgaactggc agtctgacaa actgctgcag
     1981 atggcggatc tggtttatac cggacggttt gatctcaatt ctgcgcgtaa cacccgtgaa
     2041 aaattattgc tgccgctggg cgatatcaaa ccgcttcagc aggcaggcgt gtatctggct
     2101 gtgatgaatc aggctggacg ttacgattac agtaatcccg cgacgctgtt tacgttaagt
     2161 gatatcggcg tttcagctca ccgttatcac aatcgtctgg atatctttac ccaaagcctg
     2221 gaaaacggcg cggcccagca aggagttgaa gtctcattat taaatgagaa agggcagact
     2281 ctgactcagg caaccagtga tgcccagggg catgtgcagc tggaaaatga taaaaacgcg
     2341 gcattactgt tggcgcgtaa agacggtcag acaacgctac tcgatttaaa acttccggcg
     2401 ctggacttag cagaatttaa cattgctggc gcgccaggct atagcaaaca gtttttcatg
     2461 tttggcccgc gcgatcttta tcgccctggt gaaacagtca tcctcaatgg tttattgcgt
     2521 gatgcagatg gtaaagcatt gcccgatcaa cccatcaagt tagacgtgat taaacccgac
     2581 gggcaggtac tcaggagcgt cgttagtcag ccggagaatg gcctctacca ctttacctgg
     2641 ccactcgata gcaatgcggc aaccggtatg tggcatattc gcgctaacac gggcgataac
     2701 cagtatcgga tgtgggattt ccacgtcgaa gattttatgc cagagcgcat ggcgctgaat
     2761 ctgaccggtg agaaaacccc gctaacgccg aaagatgaag tgagattctc cgtggtgggg
     2821 tactacctgt atggtgcgcc tgctaatggt aatactttgc aagggcaact tttcctgcgc
     2881 ccactgcgtg aagctgtgtc agccttacct ggtttcgaat tcggcgatat agctgccgaa
     2941 aatctttccc gcacgctgga tgaagttcag ttgacgctgg atgataaagg gcgtggcgaa
     3001 gtttctacag aaagccagtg gaaggaaacg cattccccat tacaggttat tttccagggt
     3061 agtttgttgg aatcgggcgg tcgcccggtg acgcgccgcg ctgagcaggc tatctggcct
     3121 gccgatgcat tgccggggat ccgtccgcag ttcgcctcga aatcggttta cgattatcgc
     3181 actgacagca cggtgaaaca gcccattgtt gatgaaggca gtaacgccgc ttttgacatc
     3241 gtttatagcg atgcgcaagg cgtaaaaaaa gccgtgtcgg gcttgcaggt gcgcctgatt
     3301 cgcgaacgcc gcgattacta ctggaactgg tcagaagatg aaggctggca gtcacagttt
     3361 gatcaaaaag atctgatcga aaatgaacaa actctggatc tgaaagcgga cgaaaccggc
     3421 aaggtcagtt tcccggtaga gtggggcgct tatcgtctgg aagtcaaagc gccgaatgaa
     3481 gcggtcagta gtgtgcgttt ctgggctggc tatagctggc aggacaacag cgacgggagc
     3541 ggtgccgtgc gacccgaccg tgtcacgctg aaactggata aagccagcta tcgccctggc
     3601 gataccatta agttgcatat tgccgcgcca acggcgggta aaggttatgc gatggtcgag
     3661 tccagtgaag ggccgctgtg gtggcaagag attgatgttc cggctcaagg gctggatctg
     3721 acgattccgg tcgataaaac ctggaatcgt catgatctgt atttaagtac gctggtggta
     3781 cgtcctggcg ataaatctcg ctccgcgacg ccaaaacgcg cggttggggt gttgcatctg
     3841 ccgcttggtg atgaaaaccg tcgcctcgat ctggcgctgg aaacccctac caaaatgcgc
     3901 cccaatcagc cattaaccgt gaaaattaaa gccagcaata aaaatggtga gatgccaaaa
     3961 caggtgaatg tgctggtgtc tgccgttgat agcggtgtgc tgaatattac tgattacgtc
     4021 acgccagatc cgtggcaggc gttctttggt cagaaacgct atggcgcaga catttacgat
     4081 atttacggtc aggtcattga aggtcagggg cgtctggcag ctctgcgttt tggtggtgat
     4141 ggtgatgagc tgaaacgtgg tggtaaaccg ccggtcaatc acgtcaatat tgtcgcgcag
     4201 caggcgctgc cggtaacgct caacgaacag ggcgaaggct cggttacact gccaattggc
     4261 gattttaacg gtgaattgcg cgtcatggcg caagcctgga cggcagatga cttcggaagc
     4321 aacgaaagca aagtgattgt tgctgcaccg gtgattgctg aactgaacat gccacgcttt
     4381 atggcgagtg gcgatacctc gcgcctgacg ctggatatca ctaatcttac cgataaaccg
     4441 caaaaactta atgttgccct gaccgccagt ggtttgcttg aactggtcag cgattcaccc
     4501 gcagccgttg aattagcgcc aggggtgcgt actacgctgt ttatcccggt gcgtgcattg
     4561 ccgggttatg gcgatggaga cattcaggcc accattagcg ggttagcgtt acccggggaa
     4621 accgttgccg atcagcataa gcagtggaaa atcggcgtcc gtccggcatt cccggcacaa
     4681 acggtcaatt acggtacggc gttacagcct ggtgaaacct gggcgctccc ggctgatgga
     4741 ttgcaaaact tctcgcctgt tacgctggaa gggcaattgt tgttgagcgg caaaccaccg
     4801 ctgaacatcg cacgttatat caaagagtta aaagcgtatc cgtacggctg tctcgagcaa
     4861 accaccagcg gcctgttccc gtcactttat accaatgcag cccaactgca ggcgttgggc
     4921 atcaaaggcg acagtgatga gaaacgccgt gcatcggtcg atatcggcat ttcccgtttg
     4981 ctgcaaatgc aacgtgataa cggcggcttt acgctgtggg ataaaaacgg tgacgaagag
     5041 tactggctga cggcttacgt gatggatttc ctggtccgcg caggcgaaca gggttacagc
     5101 gtaccgacag acgccattaa ccggggtaat gagcgtctgc tgcgctattt acaagatccg
     5161 ggcatgatgt cgatcccgta cgctgataat ctcaaagcca gtaaattcgc cgtacagtct
     5221 tacgctgcgc tggtgttggc ccgtcagcaa aaagcaccac tgggtgcgct gcgtgaaatc
     5281 tgggagcatc gtgcagatgc cgcttctggt ttaccgctgc tgcaacttgg cgttgcgctg
     5341 aaaaccatgg gtgatgcaat gcgtggtgaa gaagcgattg tgctggcgct gaaaacgccg
     5401 cgtaatagtg atgagcggat atggctgggt gattacggta gtccactgcg cgacagcgcg
     5461 ttgatgctct cattgctgga agagaacaaa ctgctacccg atgagcagta ctccctgttg
     5521 aacacacttt cgcagcaggc gttcggtgaa cgctggctat cgacgcagga aagtaacgcg
     5581 ttgttcctgg ctgcccgtac tcttcaggat ttaccgggca aatggcaggc gcaaaccact
     5641 ttctctgctg agccgctgac aggcgagaaa gcgcaaacca gcaatctgaa tagcgatcaa
     5701 cttgccacct tgcaggtgac caacagtggc gatcagccgt tatggctgcg tgtggatgcc
     5761 agcggttatc cgcaatccgc acctttatcg gcgagcaatg tgctgcaaat tgagcgtcat
     5821 attctgggta ctgacggtaa gagcaaatcg ctggactcgt tacgtagcgg cgatctggtg
     5881 ctggtgtggt tgcaggttaa agccagtaac agtgtaccgg atgcgttagt cgtggatctg
     5941 ttgcctgcgg ggctggaact ggaaaaccag aatctggcga acggcagcgc cagcctggag
     6001 caaagtggtg gtgaagtgca gaacttgctg aaccagatgc agcaggcgag cattaagcac
     6061 atagagttcc gtgacgatcg ctttgtggcg gcggttgccg ttgatgaata tcaaccggta
     6121 acgctggtgt atctggcgcg ggcggtgacg ccgggaacgt atcaggtgcc gcagcctatg
     6181 gtggaatcaa tgtatgttcc tcaatggcgg gcgactggtg cggctgacga tctgttgata
     6241 gtcagaccgt aaatgcctcg cctgttaacc aaacgcggct gctggataat gctggcagcc
     6301 gcgcctttta tcatcattct ggcagcatgg gcagcagata aactctggcc tctgccattg
     6361 caagaagtga atcctgcacg cgtggtggtg gcgcaggatg gtacgccact ctggcgtttc
     6421 gccgatgctg acggtatctg gcgttatccg gtaacaatcg aagatgtttc tccacgttac
     6481 cttgaagcgc tgatcaatta tgaagatcgc tggttctgga tgcatccggg ggtgaatccg
     6541 ttctcggtgg cgcgtgcagc ctggcaagat ctcacgtcgg gtcgggtcat ttccggcggc
     6601 agcacgctca ccatgcaggt ggcgcgcctg cttgatcctc accccaaaac gtttggcggc
     6661 aaaattcgcc agctctggcg cgcgttgcaa ctggaatggc atctgtctaa acgtgaaatt
     6721 ctgaccttgt atcttaaccg tgccccgttt ggcggtacgt tgcaggggat tggtgcggca
     6781 agttgggctt acctcgggaa atcgcctgcg aatttaagct attccgaggc ggcaatgctg
     6841 gcggttttgc cgcaagcgcc cagccgtctt cgcccggatc gttggccgga gcgtgccgaa
     6901 gccgcgcgta ataaagtgct cgaacggatg gccgtgcaag gtgtgtggtc ccgtgagcgg
     6961 gtaaaagagt caagggaaga acccatctgg ctggcacccc gacaaatgcc gcaactggca
     7021 ctgctgtttt cgcgcatgat gctcggtaaa agcaaaagcg acaaaatcgt tactacgctg
     7081 gatgccggtc ttcaacgacg tctggaagaa ctggcgcaaa actggaaagg acgattgcca
     7141 ccgcgcagct cactggcgat gatcgtggtt gatcataccg atatgcgtgt tcgcggctgg
     7201 gtgggatcag ttgatctcaa cgatgattca cgctttggtc atgttgatat ggtcaatgcg
     7261 atccgatcgc caggatcggt gctcaaaccg tttgtttatg gtctggcgct ggatgaaggc
     7321 ttgatccacc cggcatcgct gttgcaagac gtccccagac gcaccggtga ttaccgacct
     7381 ggcaactttg acagcggttt tcatggcccg atcagcatga gtgaggcgct ggtgcgatcg
     7441 ctgaacttac ctgctgtgca ggtgctggaa gcctatggac cgaaacggtt tgcggcaaag
     7501 ttacgcaatg ttggattgcc gttatatttg cccaacggtg ctgcaccgaa tctttcgctc
     7561 attctcggcg gcgctggcgc aaaactggaa gatatggcgg cagcgtatac cgcgtttgct
     7621 cgccacggca aggcaggcaa attgcgctta cagcctgacg atccgctgct tgaacggcct
     7681 ttaatgtcgt cgggggctgc gtggatcatt cgccggatta tggctgatga agcgcaaccc
     7741 ttgccggatg gtgccttgcc gcgcgtcgcc ccactggcat ggaaaacggg caccagctat
     7801 ggctatcgtg atgcctgggc gattggggtt aacgctcgct atgtcattgg gatctggact
     7861 ggcagaccgg acggcacgcc cgttgttggt cagtttggct ttgccagtgc cataccgctg
     7921 ttgaatcagg ttaataatat cttactgtcg cgcagtgtga atttgccgga agacccacgt
     7981 cctgactctg taagtcgtgg cgttatctgc tggccgggcg gacagtcttt ggcggaaggt
     8041 gacggtaact gtcgccgccg cctggcaacc tggctgctgg acgggagcca gccgccaact
     8101 ctattactgc cggagcagga aggcattaat ggcattcgtt tccccatctg gctggatgaa
     8161 aacggcaaac gtgttgccgc tgattgccct caggcgcgac aagaaatgat aaatgtctgg
     8221 ccgctaccgc tggaaccctg gctgcccgca tcagaacgtc gcgctgtgcg cttgccaccg
     8281 gcctcgacaa tttgcccgcc atacggtcac gatgcacaac tcccgctgca gctaacaggt
     8341 gcgcgcgatg gcgcgattat taaacgttta ccgggcgcag cggaagcgac tttgccgttg
     8401 caatcaagtg gaggggcagg tgaacgctgg tggtttctga acggcgaacc gttaactgaa
     8461 cgtgggcgca acgtgaccct gcatttgacg gataaaggcg attatcaatt gctggtgatg
     8521 gatgaggtgg ggcaaatagc gacagtgaaa tttgtcatgc aatagtctgt tttgtccctg
     8581 attgttgctt aaactgggtt tattttaaaa aaatgttacc ttcatcaata gtcaacggct
     8641 ctgttgctca ttataatccg cgccatctcg tacgctggta cagacaacaa cagaacaatt
     8701 tacagaggta aaaatggcta ttgaacgtac tttttccatc atcaaaccga acgcggtagc
     8761 aaaaaacgtg attggtagta tctttgcgcg ctttgaagct gcagggttca aaattgtcgg
     8821 caccaaaatg ctgcacctga ccgttgaaca ggctcgtggc ttttatgctg aacacgatgg
     8881 aaaaccgttc tttgatggtc tggttgaatt catgacttct ggcccgatcg ttgtttccgt
     8941 gctggaaggt gaaaatgctg ttcagcgtca ccgcgatctg ctgggcgcga ccaatccggc
     9001 aaacgcactg gccggtactc tgcgtgctga ttacgctgac agcctgaccg aaaatggtac
     9061 ccacggttct gattccgtcg aatctgccgc tcgcgaaatc gcttatttct tcggcgaagg
     9121 cgaagtgtgc ccgcgcaccc gttaataatt tcgtaaatgc cgcgtgcaaa cgtggcatcc
     9181 gtgcgccaga atttgtacaa tgcagcgccc ccggacgagc agccgctcac cggggcgttt
     9241 cttttttcaa ccctccaggg gccataacgt gtaataacga ggccggaata gcatatgtct
     9301 gaacaattag tcacacctga aaacgtcacc actaaagatg gaaaaatcaa cctgctggat
     9361 ctcaaccgtc agcagatgcg ggagtttttt aaagatttag gtgaaaaacc cttccgcgcc
     9421 gatcaggtga tgaagtggat gtatcactat tgctgcgaca actttgatga gatgaccgac
     9481 atcaacaaag tgttgcgcgg caaactgaaa gaggtggcgg agatccgcgc accggaagtg
     9541 gttgaagaac agcgttcatc tgacggcacc attaaatggg caattgccgt tggcgatcag
     9601 cgcgtcgaaa cggtgtatat cccggaagac gaccgtgcca cgctctgcgt ctcttcgcag
     9661 gtggggtgtg cgctggagtg taaattctgt tccaccgccc agcagggctt taaccgcaac
     9721 ctgcgggtat cggaaattat cggccaggtg tggcgtgcgg cgaaaattgt cggcgcagca
     9781 aaagtcaccg gtcagcgccc aatcactaac gtcgtgatga tgggtatggg cgagccgctg
     9841 ctcaacctga acaacgtcgt tccggcgatg gaaatcatgc ttgatgattt cggttttggc
     9901 ctgtcaaaac gtcgcgtcac gctttccact tccggtgtgg ttccggcgct ggataaactg
     9961 ggcgatatga tcgacgttgc gctggctatc tccctgcacg cgccgaacga cgaaattcgt
    10021 gacgaaattg tgccgatcaa caaaaagtac aatatcgaaa cgttccttgc ggcggtgcgc
    10081 cgttatctgg agaaatccaa cgccaatcag ggccgagtca ctattgaata cgtgatgctt
    10141 gaccacgtta acgacggcac tgaacacgcg caccaactgg cggaactgct gaaagatacg
    10201 ccgtgtaaga tcaacctgat cccgtggaac ccgttcccgg gcgcgccgta tggacgcagt
    10261 tcgaacagcc gtatcgaccg tttctcaaaa gtgctgatga gctacggttt caccactatt
    10321 gtgcgtaaaa cgcgtggtga tgacattgat gctgcctgtg gtcagttggc gggcgatgtt
    10381 atcgaccgta cgaaacgtac cctgcgtaaa cgaatgcagg gtgaagccat cgacattaaa
    10441 gcggtctgat agccattacg ccacggttac tttatgtacc gtggcgtaat cttttatgaa
    10501 tgaagtatta agttgcaatt gtctggtcaa tattaacggt gcgtttttgc tgactttaag
    10561 gcagtatgta acgatgcaac agtaagttag ccttagcgaa tgtgggcttt gtcacgagca
    10621 cacagacggt cttatactgt atgataacgg ttaacttaac ggatgtttcg cggtgtgggt
    10681 gggcatgatg gttcaccggc atctcaattc tcatttaaac gtacctgcag cgaatgaata
    10741 ctgaagccac gcacgaccaa aatgaagcac ttactaccgg cgctcgcctg cgtaatgctc
    10801 gcgaacaact aggacttagt caacaggccg ttgccgagcg actttgcctg aaggtttcca
    10861 cggtacgcga cattgaagaa gataaggcac ccgccgatct tgcttcaaca ttcctgcgcg
    10921 gatatatccg ctcttatgcg cgtctggtac atattccaga agaagaactg ctgccagggc
    10981 tggaaaagca ggctccacta cgggctgcaa aagttgcgcc gatgcagagt ttttccctcg
    11041 gtaaacgccg caaaaaacgt gacggctggc tgatgacctt cacctggctg gtattgtttg
    11101 tggttattgg cctgagcggt gcctggtggt ggcaagacca caaagctcag caggaagaga
    11161 tcaccactat ggccgatcaa tcttctgcgg aactgaataa taatcaaagc cagagtgtac
    11221 cgttagatac gtcgacaacc acagatcagg ctatggcgac cacgccgaca tcacctgtgg
    11281 ataccaccgc aaccaacacg caaacacctg ccgtaactgc gccagcacca gctgttgatc
    11341 cgcaacagaa tgcggttgtt ccaccttcgc aggcaaatgt tgataccgct gcgactccgg
    11401 caccagcggc aacaacaaca ccagatggcg ctgcgccgtt gccaaccgat caggctggcg
    11461 tgaccacgcc ggcggttgac ccgaatgcgc tggtgatgaa ctttactgcc gattgctggc
    11521 tggaagtcac cgatgctacc ggtaaaaaac tgtttagcgg tatgcagcgt aaagacggta
    11581 atttgaactt aaccggccag gcaccgtaca aactgaaaat tggtgcgcca gccgcagtac
    11641 agatccagta tcaagggaaa cctgtcgatc tgagtcgttt tatcagaact aaccaggttg
    11701 cgcgtctgac cctcaatgcc gaacaatcac cggcgcagta acagacgggt aacgcgggag
    11761 atttttcatg cataaccagg ctccaattca acgtagaaaa tcaacacgta tttacgttgg
    11821 gaatgtgccg attggcgatg gtgctcccat cgccgtacag tccatgacca atacgcgcac
    11881 gacagacgtc gaagcaacgg tcaatcaaat caaggcgctg gaacgtgttg gcgctgatat
    11941 cgtccgtgtc tccgtaccga cgatggacgc ggcagaagcg ttcaaactca tcaaacagca
    12001 ggttaacgtg ccgctggtgg ctgacatcca cttcgactat cgcattgcgc tgaaagtagc
    12061 ggaatacggc gtcgattgtc tgcgtattaa ccctggcaat atcggtaatg aagagcgtat
    12121 tcgcatggtg gttgactgtg cgcgcgataa aaacattccg atccgtattg gcgttaatgc
    12181 cggatcgttg gaaaaagatc tgcaagaaaa gtatggcgaa ccgacgccgc aggcgttgct
    12241 ggaatctgcc atgcgtcatg ttgatcatct cgatcgcctg aacttcgatc agttcaaagt
    12301 cagcgtgaaa gcgtctgacg tcttcctcgc tgttgagtct tatcgtttgc tggcaaaaca
    12361 aatcgatcag ccgctgcatc tggggatcac cgaagccggt ggtgcgcgta gcggggcagt
    12421 aaaatccgct attggtttag gtctgctgct gtctgaaggc atcggcgaca cgctgcgcgt
    12481 atcgctggcg gccgatccgg tcgaagagat caaagtcggt ttcgatattt tgaaatcgct
    12541 gcgtatccgt tcgcgcggga tcaacttcat cgcctgcccg acatgttcgc gtcaggaatt
    12601 tgatgttatc ggtacggtta acgcgctgga gcaacgcctg gaagatatca tcactccgat
    12661 ggacgtttcg attatcggct gcgtggtgaa tggcccaggt gaggcgctgg tttctacact
    12721 cggcgtcact ggcggcaaca agaaaagcgg cctctatgaa gatggcgtgc gcaaagaccg
    12781 tctggacaac aacgatatga tcgatcagct ggaagcgcgc attcgtgcga aagccagtca
    12841 gctggacgaa gcgcgtcgaa ttgacgttca gcaggttgaa aaataataac gtgatgggaa
    12901 gcgcctcgct tcccgtgtat gattgaaccc gcatggctcc cgaaacattg agggaagcgt
    12961 tgagggttca tttttatatt cagaaagaga ataaacgtgg caaaaaacat tcaagccatt
    13021 cgcggcatga acgattacct gcctggcgaa acggccatct ggcagcgcat tgaaggcaca
    13081 ctgaaaaacg tgctcggcag ctacggttac agtgaaatcc gcttgccgat tgtagagcag
    13141 accccgctat tcaaacgtgc gattggtgaa gtcaccgacg tggttgaaaa agagatgtac
    13201 acctttgagg atcgcaatgg cgacagcctg actctgcgcc ctgaagggac ggcgggctgt
    13261 gtacgcgccg gcatcgagca tggtcttctg tacaatcagg aacagcgtct gtggtatatc
    13321 gggcctatgt tccgtcacga gcgtccgcag aaagggcgtt atcgtcagtt ccatcagttg
    13381 ggctgcgaag ttttcggtct gcaaggtccg gatatcgacg ccgaactgat tatgctcact
    13441 gcccgctggt ggcgcacgct gggtatttcc gagcacgtaa ctcttgagct gaactctatc
    13501 ggttcgctgg aagctcgtgc gaattaccgc gatgcgctgg tggcattcct tgagcagcat
    13561 aaagaaaagc tggacgaaga ctgcaaacgc cgcatgtaca ctaacccgtt gcgcgtactc
    13621 gattcaaaaa atccggaagt gcaggtgctt ctcaacgacg ctccggcatt aggcgattat
    13681 ctggacgagg aatctcgtga gcactttgcc ggtctgtgca aactgcttga gagcgcgggt
    13741 atcgcttaca ccgtaaacca gcgtctggtg cgcggtctgg attactataa ccgtaccgtt
    13801 ttcgagtggg tgactaacag cctcggttcc cagggcaccg tgtgtgcagg tggtcgttat
    13861 gacggtctgg tggaacaact gggtggtcgt gcaacaccgg cagtcggttt tgcgatgggc
    13921 ctcgaacgtc ttgtattgtt agttcaggcc gttaatccgg aatttaaagc cgatcctgtt
    13981 gtcgatatat acctggtggc ttcaggtgct gacacacaat ctgcggctat ggcattagca
    14041 gagcgtctgc gtgatgaatt accgggcgtg aaactgatga ccaaccacgg cggcggcaac
    14101 tttaagaaac agtttgcccg tgctgataaa tggggtgccc gcgttgctgt ggtgctgggt
    14161 gagtctgaag tggctaacgg cacagcagta gtgaaggatt tgcgctctgg tgagcaaact
    14221 gcagttgcgc aggatagcgt agccgcgcat ttgcgcacgt tactgggtta aggaaggaga
    14281 aggacagcgt ggaaatttac gagaacgaaa acgaccaggt tgaagcggtt aaacgctttt
    14341 ttgctgaaaa tggcaaagca ctggctgttg gggtgatttt gggcgttggc gcactgattg
    14401 gctggcgcta ctggaatagc catcaggttg attctgcacg ctccgcttct cttgcctatc
    14461 aaaatgcggt taccgcagtg agcgaaggca aaccggatag catcccagcg gcggaaaaat
    14521 ttgctgctga aaataaaaat acttatggtg cgctggcttc tttggaactt gcgcagcaat
    14581 ttgttgacaa aaatgaacta gagaaagctg ccgcccagtt acaacagggg ctggcagaca
    14641 cgagcgatga aaatctcaaa gccgtgataa atctgcgtct tgctcgcgtt caggtacagc
    14701 tcaagcaggc tgatgccgcg ctaaaaaccc ttgatgctat caaaggtgaa gggtgggctg
    14761 ccattgttgc cgacctgcgt ggtgaagcat tgctgagcaa aggtgataag caaggtgcgc
    14821 gtagtgcatg ggaagcaggc gtgaaaagcg atgttacccc ggcactgagc gaaatgatgc
    14881 agatgaaaat taataatttg tccatctgag agggacccga tgcaattgcg taaattactg
    14941 ctgccaggac tgctttccgt taccctttta agcggctgtt cgctgtttaa cagcgaagaa
    15001 gatgtggtaa agatgtcccc attgccaacc gttgaaaacc agtttacgcc gactactgcg
    15061 tggagcactt ccgttggtag cggcattggc aacttctatt ccaatcttca tccggcactg
    15121 gcggacaacg ttgtctatgc agcggaccgc gctggtttag taaaagcgct gaatgcggat
    15181 gacggcaaag aaatctggtc tgtcaacctg gccgagaaag atggctggtt ctctaaagat
    15241 cctgcattac tttctggcgg cgtgaccgtg tctggtgggc atgtctacat tggcagcgaa
    15301 aaggcgcagg tttacgcgct gaataccagc gatggtactg tggcatggca aactaaagtc
    15361 gcgggtgaag cactttcgcg cccggtggtc agcgacggtc tggtgttaat ccacaccagt
    15421 aacggtcagt tacaagcgct gaacgaagct gacggcgctg tcaaatggac agttaacctc
    15481 gatatgccgt cgctctcttt gcgtggcgag tctgcgccgg caacggcttt tggtgcggcc
    15541 gtcgtgggtg gcgataatgg tcgcgtcagc gcagtgctga tggaacaggg ccagatgatt
    15601 tggcagcagc gtatttccca ggcgaccggt tctaccgaaa ttgaccgtct gagcgatgtt
    15661 gacacgactc ccgtcgttgt taacggcgtt gttttcgcgc tggcctataa tggtaacctg
    15721 acggcgcttg atctgcgcag cggtcagatt atgtggaaac gcgaactggg ttcggtgaat
    15781 gatttcatcg tcgacggcaa ccgcatctat ctggtcgatc aaaatgaccg ggtgatggcg
    15841 ttgaccattg atggcggcgt tacgctgtgg acacaaagcg atctgctgca tcgcctgctg
    15901 acttctccgg tgctgtataa tggcaacctg gtggtaggtg acagtgaagg ttatctgcac
    15961 tggattaacg tcgaagatgg tcgtttcgtt gcccagcaaa aagttgatag ttccggtttc
    16021 cagactgaac cggttgctgc tgacggcaaa ctgctgatcc aggcaaaaga cggaaccgtg
    16081 tactctatta cacgttaatc gtctctgtcg ttcactttga aaacggctcc tggacagggg
    16141 ccgttttcct gtttttaaca acgacacgaa tatagcgtgc gttgtctgat gatttataaa
    16201 aatgaggctt taaacatggt acctgtggtc gcgcttgtcg ggcgccctaa cgtaggaaaa
    16261 tccacgttat ttaaccgtct aactcgcacc cgagatgcgc tggttgcgga tttcccgggt
    16321 ctgactcgtg accgtaagta cggtcgtgcg gaaattgaag gccgcgagtt tatctgcatt
    16381 gataccggcg ggattgatgg caccgaagac ggtgtagaga cacgtatggc ggaacagtcg
    16441 ctgctggcga ttgaagaagc ggacgttgta ctgtttatgg tggatgcgcg cgcgggcctg
    16501 atgccggcgg atgaagcgat tgctaaacat ctgcgctccc gtgaaaaacc gaccttcctg
    16561 gtggcaaaca aaactgacgg tctggatccc gatcaggcag tggttgattt ctacgcgctt
    16621 ggtttaggtg aaatctaccc gatcgccgcg tctcacggtc gtggcgtatt aagtctgctg
    16681 gagcacgtgc tgctgccgtg gatggaagat ctcgcaccgc aagaagaagt cgacgaagac
    16741 gccgaatact gggcgcaatt tgaagcggaa gagaacggcg aagaagaaga ggcagacgac
    16801 ttcgacccgc aaagtctgcc gatcaaactg gcgattgtgg gtcgtccgaa cgtaggtaag
    16861 tctacactca ctaaccgtat tcttggtgaa gagcgcgttg ttgtttacga catgcctggc
    16921 acgacgcgtg acagtatcta cattccaatg gaacgcgacg gacgtgagta tgtgctcatt
    16981 gacaccgctg gtgtacgtaa acgcggcaaa atcaccgatg cggtagagaa attctcggta
    17041 atcaaaacgt tgcaggccat tgaagatgcc aacgtggtga tgttggtgat tgatgcgcgt
    17101 gaaggtattt ccgatcagga tctctccttg ctgggcttta ttctcaatag tgggcgctca
    17161 cttgtgattg tagtgaacaa gtgggacggc ctgagccagg aagtgaaaga gcaggtgaaa
    17221 gagacgctgg acttccgtct gggctttatc gattttgctc gtgtgcactt tatctctgcc
    17281 ttgcacggca gtggtgttgg taacttgttt gaatcagtac gtgaagcgta tgacagctcc
    17341 acccgtcgtg tggggacctc tatgctgacg cgcatcatga cgatggctgt tgaagatcac
    17401 caaccaccgc tggtacgcgg tcgtcgtgtg aagctgaaat acgcccacgc cgggggttat
    17461 aacccgccga ttgtggtgat tcacggcaat caggtgaaag acctgcctga ttcctacaag
    17521 cgttacctga tgaactactt ccgcaaatcg ctggacgtaa tgggatcgcc gattcgtatt
    17581 cagttcaaag aaggggaaaa cccgtatgcg aacaagcgta acaccctgac gccaacccag
    17641 atgcgtaaac gtaagcgtct gatgaagcat atcaagaaaa gtaagtaatc acttatctgg
    17701 tttccctttc cacacggaga gggaagccga ataacacgcg aggagtattt atggcactca
    17761 actgtccaac ctgcaaaacc cctctcgaac gcaatggcga gactgctcac tgtgcaactt
    17821 gcgacaaaga ctttaccgtc caggcacttt gccctgagtg tcatcaggcg ttacaagtgt
    17881 taaaagcttg tggtgctgtg gattatttct gccaacatgg gcacggttta atatctaaaa
    17941 aacgcgttga atttattatt gcttaatatc atgtgaatgt attgttttat catttatatt
    18001 taatgcatga atggtaaaac aatttgttat tagtggaata actaatgcaa ttgagtgaga
    18061 tcactaattt aaataattaa gaata
//
LOCUS       DAZGQL010000096        17934 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.096, whole genome shotgun sequence.
ACCESSION   DAZGQL010000096 DAZGQL010000000
VERSION     DAZGQL010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17934
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.096"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            188..754
                     /locus_tag="ACHKIR_003939"
     CDS             188..754
                     /locus_tag="ACHKIR_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO9991616.1"
                     /translation="MSKFVKAAVATAMLMGALTSASVVAAGNNGTVRFYGTIEDSPCS
                     IVPDDHKLEVDMGSIGTGSLTGGKTTTPKDFQIRLQDCNFNTETTMTTTFTGNPYSGN
                     SKNYSLSNMDNGTEIPNVSLVIGDQRGTGYDLGAGIDQDIAMDSSTGKGKQKQTLNFK
                     AWLVGETAAVTPTPAPFETLTTFQITYL"
     gene            840..3479
                     /locus_tag="ACHKIR_003940"
     CDS             840..3479
                     /locus_tag="ACHKIR_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFO9991617.1"
                     /translation="MPEHSFLRLRGLSWWIALAISGSPFNVLADDTIQFDARFLELKG
                     NTKIDLGRFSQKGYVEPGKYNLRVHVNNHPLPDEYDIYWYVAENDPNKSYACLPPELI
                     AQFGFKDDFAKSLQWGHDGQCLKTDQIGGMEIKGDLSQSALLVSVPQAYLEYTDDDWD
                     PPSRWDEGIPGLIADYSINAQTRHENGGDDTNDISGNGTVGVNVGPWRLRADWQSDYQ
                     HTRSNDDGDTDDSGTQKNWEWSRYYAWRALPSLKAKLSLGEDYLNSDIFDGFSYIGGS
                     ISTDDQMLPPNMRGYAPDISGVARTTAKVTVTQMGRVIYETQVPAGPFRIQDIGDSVS
                     GTLHVRIEEQNGQVQEYDVSTASMPFLTRPGQVRYKVTMGRPQNWDHQVAGSFFSGGE
                     ASWGIANGWSLYGGALADENYQSAALGLGRDLALFGALAFDVTHSRVQLDDNSVYGNK
                     TLDGNSYRVSYAKDFDELNSRVTFAGYRFSEKNYMTMSEYLDANDTDRARTGNDKEMY
                     TVTYNQNFRDARVSVYLNYSHHTYWDREDQTNYNMMLSHYFNLGSLRNLSVSLTGYRY
                     EYDKSADKGVYLSLSLPWGDNSTISYNGNYGSGADSSQASLYHRIDDASHYTLSAGTS
                     ENHTSLDGYYSHDSTLAKVDLSANYHEGQYTSAGVSLQGGVTLTAHGGALHRTQNMGG
                     TRLLIDADGVANVPVEGNGSAVYTNMFGKAVVADVNDYYRNQAYIDLNKLPENAEATQ
                     SVVQATLTEGAIGYRKFAVISGEKAMVVLRLQDGSHPPFGAEVKNDNQQQVGLVDDEG
                     NVYLAGVKPGEHMTVFWEGESHCDISLPDPLPNDLFNGLLLPCQQKGGSAPVITHDIQ
                     PVIQEQTQQVTPMEPPMSVSSNQ"
     gene            3499..4248
                     /locus_tag="ACHKIR_003941"
     CDS             3499..4248
                     /locus_tag="ACHKIR_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO9991618.1"
                     /translation="MTKHLSVKTLATVLSLLAATVSFSALASVTPDRTRLIFNESDKS
                     ISVTLRNNDPKQPYLAQSWIEDEKGVKISSPLAVLPPVQRIDAMMNGQVKIQGLPDIH
                     KLPADRESIFYFNVREIPPKSNKANTLQIALQTRIKLFWRPKALEKASMQNPWQHKVT
                     LTRNGQEFTVNNPTPYYVIFSNASAQKNGNPADGFSPIVMSPKTSAPLKVKMASVPVL
                     TYVNDYGARMPLFFTCSGNSCQVDEELSRKG"
     gene            4265..4771
                     /locus_tag="ACHKIR_003942"
     CDS             4265..4771
                     /locus_tag="ACHKIR_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO9991619.1"
                     /translation="MKRSCSVWAGTWLLMCCIGSDFAVAGSKSVNLSLRVLVDAPPPC
                     TVNGATVEFGNVFINKIDGVGYKQPIDYSLVCNNLAMDDLRLQMRATTVVINGETVIS
                     TGISGFGIRVQKSSDHSTLDLTSGSWLPFNFSSGVPALEAVPVKQSGTTLSAAEFNAS
                     ATIVVDYQ"
     gene            4768..5238
                     /locus_tag="ACHKIR_003943"
     CDS             4768..5238
                     /locus_tag="ACHKIR_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO9991620.1"
                     /translation="MKRISLLMLWGFSSMALSNVSFHGYLVQPPNCTISNGKTIEITF
                     QDVFVDDINGSNYEQTVPYSITCDTAVRDPSMEMKLSWSGTQSDFDDAAVSTDISGLA
                     IRLKQAGQSFKLNTALVVNETALPVLTAVPVKKSGVDLPEADFEAWATLQVDYQ"
     gene            5235..5759
                     /locus_tag="ACHKIR_003944"
     CDS             5235..5759
                     /locus_tag="ACHKIR_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO9991621.1"
                     /translation="MKKKRALVFICSLMLLYSVTAPAVDNLHFTGNLLGKSCTPVING
                     SLLAEVHFPTIAASDLMNLGQSERVPLVFQLKDCKSSTLFSVRVTLTGTEDSELPGFL
                     AFDASSSASGAGIGIETAAGAAVPVNSTTGVTFPLNQGNNSLNFNAWLQAKGGRDVTS
                     GDFSATATATFEYF"
     gene            5761..6603
                     /locus_tag="ACHKIR_003945"
     CDS             5761..6603
                     /locus_tag="ACHKIR_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001317961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StfH/YfcO family fimbrial adhesin"
                     /protein_id="HFO9991622.1"
                     /translation="MKKWTMIFASLVLLVMSASVPKSYAGDKLTSASFDSVKIYYGKG
                     KMSIHNTGGLYVTVLTPNEAAYGVYNDAIRKNEQASVISWTGSGPAPVLILTDYGNAD
                     SSNCPGINTATFQCAYETFKITVQSDSYGCPWLASFYTITDQPGFGRYTGPTNHATVC
                     PTIPVASYDISWSENYVSHNKALQLQSNGSTITTTLSTYLMEDGKLCDGSVFDSRGAY
                     CRVVSELLTFTSYGCDKSTVTVSPTRHPVTDKQLHDIVVNVNTSSRQPIDSTCRFQYV
                     LNEL"
     gene            complement(6719..7270)
                     /gene="smrB"
                     /locus_tag="ACHKIR_003946"
     CDS             complement(6719..7270)
                     /gene="smrB"
                     /locus_tag="ACHKIR_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HFO9991623.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            7436..8368
                     /gene="prmB"
                     /locus_tag="ACHKIR_003947"
     CDS             7436..8368
                     /gene="prmB"
                     /locus_tag="ACHKIR_003947"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFO9991624.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLIAAREHFAIYKD"
     gene            8403..9488
                     /gene="aroC"
                     /locus_tag="ACHKIR_003948"
     CDS             8403..9488
                     /gene="aroC"
                     /locus_tag="ACHKIR_003948"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFO9991625.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSQVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            9491..10315
                     /gene="mepA"
                     /locus_tag="ACHKIR_003949"
     CDS             9491..10315
                     /gene="mepA"
                     /locus_tag="ACHKIR_003949"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HFO9991626.1"
                     /translation="MNKTAIVLLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSRQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVPTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPPGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            10315..11124
                     /locus_tag="ACHKIR_003950"
     CDS             10315..11124
                     /locus_tag="ACHKIR_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFO9991627.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALVAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            11124..11672
                     /gene="epmC"
                     /locus_tag="ACHKIR_003951"
     CDS             11124..11672
                     /gene="epmC"
                     /locus_tag="ACHKIR_003951"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HFO9991628.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARREQVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLANGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            11706..11984
                     /locus_tag="ACHKIR_003952"
     CDS             11706..11984
                     /locus_tag="ACHKIR_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HFO9991629.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQKASQQ"
     gene            complement(12040..14046)
                     /gene="mnmC"
                     /locus_tag="ACHKIR_003953"
     CDS             complement(12040..14046)
                     /gene="mnmC"
                     /locus_tag="ACHKIR_003953"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HFO9991630.1"
                     /translation="MKHYAIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEARFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHLHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGHVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGSTLATFTSAGFVRR
                     GLQDAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RLYDLLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPTELAVAVGANEVEGVTGVV
                     TNCGGITYPQGGWLCPAELTCNVLKLAQQQGLQIRYQHQLKDLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSSLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSEKEARCGV
                     RCATRDHLPMVGNVPDYDVTLVEYASLAEQKDEAVSAPIYDDLFMLAALGSRGLCSAP
                     LCAEILAAQMSNEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            14205..15425
                     /gene="fabB"
                     /locus_tag="ACHKIR_003954"
     CDS             14205..15425
                     /gene="fabB"
                     /locus_tag="ACHKIR_003954"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HFO9991631.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMKMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            15690..16868
                     /locus_tag="ACHKIR_003955"
     CDS             15690..16868
                     /locus_tag="ACHKIR_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9991632.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     ELGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVPAPFKFALLVVGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALALTTMALPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSH
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(16865..17860)
                     /gene="flk"
                     /locus_tag="ACHKIR_003956"
     CDS             complement(16865..17860)
                     /gene="flk"
                     /locus_tag="ACHKIR_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HFO9991633.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
BASE COUNT         4279 a         4496 c         4755 g         4404 t
ORIGIN      
        1 tgcgtcagtc atattaaaat atcaatatgt tcggcacgcc aaagaatatt atatcaagga
       61 acctgttttt tcaggagctg aaaatctgtt cattggctgc ttaaaagtcg ctgcaaaaat
      121 tgaagaagtt ctgtgtcaat atttcagttg gcttatattc taattattta tgatttaagg
      181 acaaataatg agtaagtttg taaaagcagc agttgccacg gcaatgctca tgggggcgct
      241 gacttcagca tcagtcgttg ccgcaggtaa caacggtaca gtgagatttt acggcacaat
      301 tgaagactct ccgtgctcga ttgttcctga cgatcataaa ctggaagtgg atatgggcag
      361 cattggtaca ggttctttaa ccggtggcaa gactaccaca cctaaagatt tccagattcg
      421 tttacaggat tgtaatttca atactgaaac gacaatgacc acgacattca ctggtaatcc
      481 gtactccggc aactcaaaaa actattccct gtctaacatg gataatggaa ctgaaattcc
      541 aaatgtaagt ctggtgattg gcgaccaacg tggtactggt tatgacctgg gggcaggcat
      601 cgatcaggac atcgcgatgg acagttcaac aggaaaaggc aaacagaaac aaacgttgaa
      661 ctttaaagcc tggttagtag gtgaaactgc agcggtaaca ccaaccccag cgccgtttga
      721 aacgctaacg acgttccaga ttacatatct ctaattatgc tgttccccca taactgcaat
      781 gttatggggt ttataaataa aagataacta aattataata atcatggatg gatacgtaga
      841 tgcctgaaca ttcttttctt cgtttacggg ggctttcatg gtggattgct ctggcaattt
      901 ccgggagtcc ttttaatgta ctggcagatg ataccattca atttgatgct cgatttctgg
      961 agttaaaagg aaataccaaa attgatctgg ggcgtttttc tcaaaaaggg tatgttgaac
     1021 cagggaaata caatttacgt gttcatgtaa ataatcaccc attacctgat gaatacgata
     1081 tctattggta tgtagctgaa aacgatccga ataaatcata tgcctgctta ccacctgaac
     1141 tgatagccca gtttggcttc aaagatgatt ttgcgaagag tttgcaatgg ggtcatgatg
     1201 gacaatgtct gaaaactgac caaataggag gtatggaaat taagggcgat ttaagtcaat
     1261 ctgcgctctt agtttctgtg ccacaggcat atctcgaata cactgacgat gactgggacc
     1321 caccttcgcg ttgggatgag gggattcctg ggcttattgc cgattacagt atcaacgcac
     1381 aaacccgaca tgaaaatggg ggggatgaca ctaacgacat cagcggtaat ggtacggtcg
     1441 gcgttaacgt ggggccgtgg cgcttacgag ccgactggca gagcgattac caacataccc
     1501 gcagtaacga tgacggcgac actgatgata gcggtacgca aaaaaactgg gagtggagcc
     1561 gttactacgc ctggcgcgcc ttgccgtcat taaaagccaa actttcgctg ggtgaagatt
     1621 atctcaactc cgatattttt gatggcttca gttacattgg cggcagcatc agtaccgatg
     1681 atcaaatgtt accgccaaac atgcgcggct acgcacccga tatttcaggt gttgcacgca
     1741 ctaccgctaa agtgaccgta acccaaatgg gccgtgtcat ttacgaaacc caagtgcccg
     1801 caggtccatt ccgcattcag gatattggcg attccgtatc tggcacgctg catgtccgca
     1861 ttgaagaaca aaacggtcag gtacaggaat atgacgtcag cacggcgtcc atgccgtttc
     1921 tgacccgccc cggtcaggtg cgttacaaag tgacaatggg gcgtccacaa aactgggacc
     1981 atcaggttgc aggcagcttc ttctcgggcg gtgaggcgtc atgggggatt gcgaatggct
     2041 ggtcgctcta cggcggggcg ttggccgatg aaaactacca gtcggcagcg ttaggcctcg
     2101 gtcgtgacct cgcgctgttc ggagcgctag cgtttgatgt cactcactct cgtgttcaac
     2161 tcgacgataa cagcgtgtat ggcaacaaaa cgctggacgg caactcttat cgcgtcagct
     2221 atgccaaaga tttcgatgaa ctcaacagtc gggtgacgtt tgccggatat cgcttctctg
     2281 aaaaaaacta catgaccatg agtgagtatc tggatgcgaa cgacaccgac cgtgcgcgca
     2341 cgggtaacga caaggaaatg tacacggtca cttacaacca gaactttagg gatgcgcgcg
     2401 tttcggtcta tctcaattat tctcaccata cctattggga tcgcgaggat cagaccaact
     2461 acaacatgat gctttctcat tatttcaatt taggaagtct tcgcaacctg agcgtttctc
     2521 tcaccggtta tcgctacgaa tatgataaaa gtgccgacaa aggtgtttat ctgtccttga
     2581 gtttgccatg gggcgacaac agcaccatca gttacaacgg caattatggc agtggtgccg
     2641 acagcagcca ggcgagtttg tatcaccgca tcgatgacgc tagccactat acgttgagcg
     2701 ccggaaccag tgaaaatcac accagtcttg atggttatta cagtcatgac agtacgctgg
     2761 caaaagttga cctgagtgca aactaccatg aggggcaata cacatctgcg ggggtctcct
     2821 tacagggcgg tgtaacactg actgctcacg gcggggcgtt gcatcgtacg caaaatatgg
     2881 gaggcacacg tctgctaatc gacgccgatg gcgttgctaa tgtgccggtc gaaggtaatg
     2941 gttccgccgt ttataccaac atgttcggga aagcggtggt ggcagatgtt aatgattact
     3001 accgtaacca ggcttatatc gatttgaaca aactaccgga aaacgccgaa gctacccaat
     3061 cggtggtcca ggcaacgctg acggaaggcg ctattggcta ccgtaaattt gcggtgatca
     3121 gcggtgagaa ggcgatggta gtgctgcgct tgcaggatgg cagccatccg ccatttgggg
     3181 cggaagtgaa aaacgataat caacaacaag ttgggctggt tgatgacgaa ggtaatgttt
     3241 atcttgcagg cgttaagccg ggtgaacata tgaccgtttt ctgggaggga gaatctcact
     3301 gcgatatcag cctgccagat ccgttaccga atgacctgtt caatggcttg ttgttgccat
     3361 gccagcaaaa agggggaagt gctcccgtca taacgcatga tattcaacct gtaattcagg
     3421 agcagacaca acaggtgaca ccaatggaac cgccgatgtc tgtttcatcg aaccaataac
     3481 gtgattaagg aatgatccat gactaaacat ctctctgtca aaacattggc aacggtactg
     3541 tcgttactgg ctgcaacggt aagtttttct gctctggcgt cagtaacgcc cgatcgtact
     3601 cgcctgattt ttaatgaaag cgataaatcc atcagcgtca cgctgcgtaa taacgacccg
     3661 aaacagcctt atctggcaca aagctggatc gaagatgaaa agggcgtcaa gattagttcg
     3721 ccgctggcag tattgccccc cgtacagcgc attgatgcaa tgatgaatgg tcaggtgaaa
     3781 attcagggat tgccagatat ccataaattg cctgccgatc gtgaatcgat attttatttc
     3841 aatgtgcgtg aaattccgcc gaaatcgaac aaagccaata cgttacaaat tgcgctgcag
     3901 acacgcatca aacttttctg gcgacctaaa gccctggaaa aagctagcat gcaaaaccca
     3961 tggcagcata aagtgacctt aacccgtaac ggtcaggaat ttaccgtcaa taaccctact
     4021 ccgtattacg tcattttcag taatgccagc gcgcaaaaaa atggcaatcc ggcagacggt
     4081 ttctcgccga tagtgatgtc gccaaaaacc tccgcaccgc tcaaggtgaa gatggctagc
     4141 gtgccggtgc tgacctatgt caacgactat ggcgcacgga tgccactctt tttcacctgt
     4201 agcggcaaca gttgtcaggt tgatgaagag ctgagccgca aaggttaatg acggggaagt
     4261 aagtatgaaa agatcttgtt cagtctgggc gggaacatgg ttactgatgt gttgtattgg
     4321 tagcgacttt gcagttgcag ggagtaaatc agtaaatttg agtttacgag tgctcgttga
     4381 tgcccctcct ccttgcacgg ttaatggtgc gacggttgag tttggtaatg tattcatcaa
     4441 taagatcgat ggggtaggtt ataaacaacc cattgattac tcgcttgttt gtaacaacct
     4501 ggcaatggat gacctgcgtt tacagatgcg ggcaacaacg gttgtgatca acggtgaaac
     4561 agttatcagc acaggtattt cagggtttgg tattcgggtt cagaaaagta gtgatcactc
     4621 aacactcgac ttaacgtctg gttcatggtt gccgtttaat ttttcctcgg gcgtccctgc
     4681 actcgaagca gtgccggtga aacagagtgg tacaacctta tcggcagcgg aatttaacgc
     4741 cagtgccaca attgtggtgg attaccaatg aaacggatct cacttcttat gctgtggggc
     4801 tttagctcga tggcactctc aaatgtctct tttcacggct atttagtgca gccacctaac
     4861 tgcactatta gcaacggtaa aaccattgaa ataacctttc aggatgtttt tgttgatgac
     4921 attaatggca gtaactatga gcaaaccgtg ccgtattcca ttacctgtga tacggcggtt
     4981 cgcgacccat caatggaaat gaaattgagc tggagcggta cgcagagcga ctttgacgat
     5041 gccgcggtta gcaccgatat ctctggcctg gcgattcggc ttaaacaggc agggcagagc
     5101 tttaagctca atacggcact ggtggtgaat gaaacggcct taccggtgtt aaccgcagta
     5161 ccggtcaaaa agagcggtgt cgatttgcct gaggcagact ttgaagcctg ggccacgtta
     5221 caggtggatt atcaatgaag aaaaagcgtg cgttggtttt tatctgtagc ctgatgctgt
     5281 tgtacagcgt tactgccccg gcggtcgaca acctgcactt taccggtaat ttgctcggta
     5341 aatcctgtac gccagtgatc aacggtagcc tgttggcgga ggtgcacttt cccaccattg
     5401 ccgccagcga tttaatgaac ctcgggcagt cagagcgagt accgttggta tttcaattga
     5461 aggattgcaa aagctccacg ctgtttagcg tcagggtaac gctgacggga acggaagata
     5521 gcgaactgcc cggatttctg gcctttgacg cctcatcttc tgcatccgga gctgggattg
     5581 gtattgaaac ggcggcaggc gcggcggtgc cagtcaacag cacaacgggt gtcacgttcc
     5641 cgctaaacca gggcaataac agtctcaatt ttaatgcctg gctacaggcg aagggcgggc
     5701 gggatgtgac gtccggtgat ttcagcgcta cggcgacggc aacctttgag tatttttaac
     5761 atgaaaaaat ggacaatgat ttttgcgagc ctggtgttgc tggtgatgag tgcgagcgtt
     5821 ccaaagagct atgccgggga taaactcacc agcgcgagtt tcgacagtgt gaagatttat
     5881 tatgggaagg ggaaaatgtc tattcataac accggaggtt tgtatgtcac ggtgttaact
     5941 cccaacgagg cggcctatgg ggtgtacaac gatgccataa ggaagaacga gcaagccagc
     6001 gttatttcgt ggacggggtc gggaccagcg ccagtgttga tcttaactga ctacggtaat
     6061 gctgatagct cgaattgtcc tgggataaat acggcgacat ttcagtgtgc atatgagacg
     6121 tttaagataa ccgttcagtc cgacagttat ggttgcccgt ggctcgcttc tttttatacg
     6181 atcacagatc aacccggatt cgggagatat actgggccaa cgaaccatgc cacagtatgt
     6241 ccgacgatcc ctgtcgcgtc gtatgacatc tcgtggagtg agaattacgt cagccacaat
     6301 aaggcgttac agttgcaatc caatggctcg acaatcacca ccacgctttc gacctacctg
     6361 atggaagatg gcaaactctg tgatggcagc gtttttgata gcagaggtgc atactgccgc
     6421 gtggtctcgg aactgttaac ctttacctcc tacggttgcg ataaatcaac ggtaacggtt
     6481 tctcccacac ggcatccggt aaccgataaa cagctacatg atatcgtggt aaatgtgaac
     6541 accagtagcc gacaaccgat tgattctacg tgccgattcc agtatgtact gaatgaattg
     6601 tagtgacgat attttcacaa gtcgacgggg ccatctggcc ctgtcgtcat ttccggcgtc
     6661 tgttttatga tatgatggct aaggcgaact ttccctctac agcattcgat gccagcattc
     6721 agggcaattc aggcggcagc cactcttcca cttcaatcaa caccaacaaa gccgcatcgc
     6781 caccatactc ttttggtgcc tggtgaaatg ccattacatg tggatgctgc gccagccaca
     6841 gcggcgtttg ctgcttcaaa atatgcttcc cgtgaccatg catcacgcag gcacaaaaca
     6901 catgttcacg gcggcaggcg gcaatcaacg cccccagttc ctgtttggct tgcaactgcg
     6961 tcagaccgtg caaatcaaga aacaactccg gtgaataatc accgcgccgc agtttcttcg
     7021 cttcaaaatg gctgacatcc gggcgaacat atttcaccgg accttcggta tttaataatg
     7081 gctgaaactc atcggaaaaa taatggctgg catcggcctg ctcctgaatc aaccgcttga
     7141 ccggtacttc gctgattttt ttacgctgcg gtcggtggac aatcgtgtcc tgcttaatct
     7201 tgcgagtccc cgccatcaac tggcgaaaca gcgcctggtc ctcctcgcta agtgttgttt
     7261 tctttttcat ttaactgtct ctatttctaa ttttcctaca gtttacctga ctcgtagcac
     7321 aatcgatcgt tttaaacgta tttttcctca ctcaccgcgc gtgaatgctg atttatcgtc
     7381 atcttcatgg caaactagcc gccgaaatta ttgcgatcat gccctggagg aatacgtgga
     7441 taaaattttc gttgatgaag cagtaaatga gctgcaaacc attcaggaca tgttgcgctg
     7501 gtcggtgagc cgcttcagcg cggcgaatat ctggtacggc cacggcaccg ataacccgtg
     7561 ggatgaagcc gtgcaactgg tgttgccttc actctacctg ccgctggata ttccggaaga
     7621 tatgcgcacc gcgcgtctga cttccagcga aaaacaccgt attgttgagc gcgtgatccg
     7681 ccgcgtcaac gaacgcattc cggtggctta cctgaccaat aaagcctggt tctgtggcca
     7741 tgaattttac gtcgatgaac gcgtgctggt gccgcgctcg ccgattggtg aactgatcaa
     7801 caataaattt gccggactca tcagcaagca accgcagcac attttagata tgtgtaccgg
     7861 tagcggctgc atcgccattg cctgtgctta tgccttcccg gaagcggaag tcgacgcggt
     7921 agacatctct cccgacgcgc tggcggttgc tgaacagaac atcgaagaac acggtttgat
     7981 ccacaacgtc attccgattc gttccgactt gttccgtgat ttgccgaaag tgcagtacga
     8041 cctgattgtc accaacccgc cgtatgtcga tgcggaagat atgtccgacc tgccaaacga
     8101 ataccgccac gagccggaac tcggtctggc atctggcact gacggcctga aactgacgcg
     8161 tcgcattctt ggtaacgcgg cagattacct cgctgatgat ggcgtgctga tttgtgaagt
     8221 gggcaacagc atggtacatc ttatggaaca atatccggat gttccgttca cctggctgga
     8281 gtttgataac ggcggcgatg gtgtgtttat gctcactaaa gagcagctta ttgccgcacg
     8341 agaacatttc gcgatttata aagattaagt aaacacgcaa acacaacaat aacggagccg
     8401 tgatggctgg aaacacaatt ggacaactct ttcgcgtaac caccttcggc gaatcgcacg
     8461 ggctggcgct cggctgcatc gtcgatggtg ttccgccagg cattccgctg acggaagcgg
     8521 acttgcaaca tgaccttgat cgtcgtcgac ctgggacatc gcgctatacc acccagcgcc
     8581 gcgagccgga tcaggtcaaa attctctccg gtgtttttga aggtgttact actggcacca
     8641 gcatcggatt gttgatcgaa aataccgacc agcgttctca ggattatagt gcgattaagg
     8701 acgttttccg tccaggtcat gccgattaca cctatgaaca aaaatacggc ctgcgcgatt
     8761 atcgcggcgg tggacgttct tccgcccgcg aaaccgctat gcgcgttgca gcaggggcga
     8821 ttgccaaaaa atatctcgcc gagaaatttg gcattgagat tcgcggctgc ctgacccaga
     8881 tgggtgatat tccgctggaa atcaaagact ggtcgcaggt cgagcaaaat ccgtttttct
     8941 gcccggaccc ggacaaaatc gacgcgttag atgaactgat gcgcgcgctg aaaaaagagg
     9001 gtgactccat cggcgcgaaa gtcaccgtcg ttgccagtgg cgtacccgcc ggacttggcg
     9061 agccggtttt tgaccgcctg gatgccgaca tcgcccatgc gctaatgagc attaacgcgg
     9121 tgaaaggtgt ggaaattggt gatggttttg acgtggtagc gctgcgtggc agccagaacc
     9181 gtgacgaaat caccaaagac ggtttccaga gcaaccatgc gggcggcatt cttggtggta
     9241 tcagcagcgg gcagcaaatc attgcccata tggcgctgaa accgacctca agcattactg
     9301 tgccggggcg cacgattaac cgctttggcg aagaagttga gatgatcacc aaaggccgtc
     9361 acgatccgtg tgttgggatc cgcgcggtgc cgatcgctga agcgatgctg gcgatcgttt
     9421 taatggatca cctgttacgg caacgggcgc aaaatgctga tgtgaagact gatattccac
     9481 gctggtaaaa atgaacaaaa ctgcgattgt gctgctggcc ctgcttgcca gtagcgccag
     9541 cctggcagcg acgccgtggc aaaaaataac ccaacctgtg ccgggtagtg cacaatcgat
     9601 aggcagtttt tcaaatggct gtatcgtcgg tgctgacacg ctgccgatac agtccgaaca
     9661 ttatcaggtc atgcgtaccg atcagcgtcg ctatttcgga cacccggatc tggtgatgtt
     9721 tatccagcgt ctgagtcgcc aggtgagcaa tctgggtatg ggtacggtgc tgattggcga
     9781 tatggggatg cctgctggtg gtcgtttcaa cggcggtcac gccagccacc agactggact
     9841 ggatgtcgat atcttcctgc aactgccaaa aacacgctgg acctccgcgc agctcttgcg
     9901 cccgcaagcg ttagatttag tttcccgcga cggtaaacac gttgtcccga cgctgtggaa
     9961 gccggaaatt ttcagcttga ttaaactcgc cgcgcaggac aaagacgtca cgcgcatttt
    10021 tgttaatccg gcgattaaac aacaactttg ccttgatgcg ggcaccgatc gcgactggtt
    10081 acgcaaagtg cggccctggt tccagcatcg cgcccatatg catgtacgac tacgttgtcc
    10141 tgccgatagc ctggagtgtg aagatcaacc tttaccgcca ccgggcgatg gttgcggggc
    10201 agaactgcaa agctggtttg aaccaccaaa accgggaaca acaaagcctg agaagaagac
    10261 accgcctccg ttgccgcctt cctgccaggc gctactggat gagcacgtga tctaatggaa
    10321 acgtttaata gcctgtttat ggtttccccg ctgctgctgg gagttctctt ttttgtcgcc
    10381 atgctggcgg gatttatcga ctcgattgcc ggtggcggtg ggttactcac cattccggca
    10441 ttgatggcgg cggggatgtc tccggctaac gcgctggcaa ccaataaact gcaagcctgc
    10501 ggcggctcta tttccgccac tatctacttt attcgccgca aagtggttag tttaagcgat
    10561 cagaaactca atatcgccat gacctttgtc ggctcaatga gcggcgcatt actggtgcag
    10621 tatgttcagg ctgatgtctt acggcagatt ctgcccattc tggtgatttg tatcggcctc
    10681 tatttcttgc tgatgccaaa gctgggtgaa gaagatcgcc agcgccggat gtatggactg
    10741 ccatttgcgc tggtggcggg gggctgcgtc ggtttttacg atggattctt tgggccagct
    10801 gccggatcgt tttacgcgct ggccttcgtt acgctgtgcg gattcaacct cgccaaagcc
    10861 acggctcacg ccaaattact caacgcaacg tcaaacattg gcggtctgct gctgtttatt
    10921 ctcggcggca aagtgatttg ggcgacgggc tttgtgatgc tggtggggca gttcctcggc
    10981 gcgcgcatgg gttcaagact ggtgttgagc aaaggacaaa agctgatccg cccgatgatc
    11041 gttatcgtct cggcggtgat gagtgcaaaa ttactttacg atagccacgg acaggagatc
    11101 ctccactggt tggggatgaa ctaatgaaca gtacacacca ctatgagcag ttgattgaga
    11161 tttttaatag ctgctttgcc gatgaattta atacccgtct gattaaaggc gacgacgaac
    11221 cgatctatct tcctgctgat gcggaagtac cgtataaccg aatcgtcttt gctcatggct
    11281 tttatgccag cgcaattcat gagatttcgc actggtgtat tgccggaaaa gcgcgccgtg
    11341 agcaggttga tttcggctac tggtattgcc cggacggtcg cgatgcccag acgcaaagcc
    11401 agtttgaaga tgttgaagtg aagccgcagg cgctggactg gttgttctgc gtggcggcgg
    11461 gatatccgtt taatgttagc tgcgacaatc tggaagggga tttcgaaccg gaccgcgtgg
    11521 tgtttcaacg gcgggtacat gcgcaggtaa tggattatct ggcaaacggc attcccgaac
    11581 gtccggcacg cttcattaaa gcactacaaa attattatca cacgccggaa cttacggcgg
    11641 aacagttccc gtggccggaa gcgctcaact gaagccagcg ccacgtttta caatgaggaa
    11701 agaagatgat cgcggagttt gaatcacgca ttctggcatt aatcgacggt atggttgacc
    11761 atgccagtga tgatgagttg tttgccagtg ggtatttgcg tggccacctg acattagcca
    11821 tcgcagaact ggaaagtggt gatgaccact ccgcccaggc ggtacataca accgttagcc
    11881 agagtctgga aaaagcgatt ggtgccggtg agttgtcgcc gcgtgaccag gcgctggtga
    11941 ccgatatgtg ggaaaacctg tttcagaagg cgtcacagca gtaagtaatg tgccggataa
    12001 ggcgttcacg ccatatccgg caagcaatgc cagagctgat taccccgcct taaccgcttt
    12061 acctttcaac aacttccgca cccataaccg attcgggttt aacgccgcca gcgtactggc
    12121 atccatcgga attggttcgt tgctcatctg cgccgccaga atctcggcac acaacggggc
    12181 agagcacaaa ccgcgagaac ctaaagcagc taacataaaa agatcgtcat aaattggcgc
    12241 gcttactgcc tcatctttct gctccgccag cgaagcatat tccacgagcg ttacgtcata
    12301 atcgggcacg ttgcctacca ttggcagatg atcgcgggtg gcacaacgca caccgcagcg
    12361 cgcctctttc tcactgacat caacctcttt tgcccactgc gcctgcggga aacaatcaat
    12421 caaccgctgg cgattctgct gctgatcgtc ctcactgtac gccgtatctt cgctgccgcg
    12481 atgataactg gcaccaatac aatgatgttg attcgccgga ttttgtggcg tgagataacc
    12541 gtcatagcac agcacctgct tcagctccgc tagttccggc gttgtcggaa tatggctaac
    12601 ctgcccggca accgaataca ctggcagaga cgatgtttgg ctgaaacggc tgatttgatg
    12661 cccgttcgcc agtaccacca cgctatgcgt tgcttgctga tctcctgcaa aattcagcaa
    12721 ccaacagtca tccttacggg ataaatcctt taactggtgt tgataacgaa tctgtaaacc
    12781 ttgctgttgc gccagtttca gcacattaca ggtcagttct gctggacaca gccatccacc
    12841 ttgcggatat gtaataccgc cacaatttgt tactacacct gtaacgcctt caacctcatt
    12901 cgccccgacc gcaacagcca gttctgtggg taaatccatc gacagcatct gtgcgatttt
    12961 atgctggctt ttctcatccc agcctaactg cgtgacgccg caccagtcat gatcaaattt
    13021 gacgggcaat aaatcgtaaa gccgacgagc aaaagtaaac gcattagaga aaaagcggtt
    13081 caacgcctca tcgtgtttac ttaagagcgg atacagcgcc ccctggcgat tgccggaagc
    13141 acccagcgcg ggggcttcat ccgcgcaata aagcgttact tgccagccgc gccgtaataa
    13201 cgccagcgac aacaacgcgc tggcaatacc accgccgata atcgctactt cccgtttgct
    13261 gctgcccgta cgattaaacc acggcgtgga gcaggggagg ggtaatgtct gttccatcac
    13321 cccacaaagc atttcccgtt tgcgcccaaa gcccttacgt ttttgcatcg tgaatccggc
    13381 gtcctgcaaa ccgcggcgga caaaaccggc agacgtaaat gtcgccagcg tgctgcccgg
    13441 acgcgccagt cgtgccatgg cgttaaacag attttgcgtc cacatatccg ggtttttcgc
    13501 tggcgcaaag ccgtccagaa accaggcgtc cactttttga ttcagcgagt cgtccagttg
    13561 gctggtcagt tcgtttatat cgccaaacca taaatccagt gtcacgtggc cttcatcgag
    13621 caataaacga tggcaaccgg gcaagggcat tggccactgc gcctgaagtt gttctgccca
    13681 cggagccagt tccggccagt gtagatgcgc taaggctaaa tccgcacggg tgaggggaaa
    13741 tttctcaaaa ctaatgaaat gtaagcgttg taattgcgct tgcggatgtg cttcgcgaaa
    13801 ctgatcaaat gcctgccata gtgtcaggaa gtttaatccg gtgccgaagc cgctctctgc
    13861 taccacaaac agaggatgtg gatgctcagg aaagcgtgcc tctaattggt tacctccgag
    13921 aaaaacataa cgcgtctctt ccagtccgtt gtcattggaa aagtaaacgt cgtcaaaatc
    13981 tcgggaaaca ggtgtaccct cagcattaaa ttcgaggttg gcaggttgta tggcgtagtg
    14041 tttcacgtaa gttactcgtc tgacgggcag tctgacgatc ttaacgatgt gtgtcaggct
    14101 gcgcaaattt ctctattaat tggctgatcg gacttgttcg gcgtacaagt gtacgctatt
    14161 gtgcattcga aacttactct atgtgcgact tacagaggta ttgaatgaaa cgtgcagtga
    14221 ttactggcct gggcattgtt tccagcatcg gtaataacca gcaggaagtc ctggcatctc
    14281 tgcgtgaagg acgttcaggg atcactttct ctcaggagct gaaggattcc ggcatgcgta
    14341 gccacgtctg gggcaacgta aaactggata ccactggcct cattgaccgc aaagttgtgc
    14401 gctttatgag cgacgcatcc atttatgcat tcctttctat ggagcaggca attgcagatg
    14461 ctggcctctc tccggaagct taccagaata acccgcgcgt tggcctgatt gcaggttccg
    14521 gcggcggttc cccgcgtttt caggtgttcg gcgctgacgc aatgcgcggc ccgcgcggcc
    14581 tgaaagcggt tggcccgtat gtggtcacca aagcgatggc atccggcgtt tctgcctgtc
    14641 tcgccacgcc gtttaaaatt cacggtgtaa actactccat cagctccgcg tgtgcgacct
    14701 ccgcacactg tatcggtaac gcagtagagc agatccaact gggcaaacag gacatcgtgt
    14761 ttgctggtgg cggcgaagag ctgtgctggg aaatggcttg tgaattcgac gcgatgggtg
    14821 cgctgtctac caaatacaac gacaccccgg aaaaagcctc ccgtacttat gacgctcacc
    14881 gtgacggttt cgttatcgct ggcggcggcg gtatggtggt ggtcgaagaa ctggaacacg
    14941 cgttggcacg tggtgctcat atctacgctg aaatcgttgg ctacggcgca acgtctgatg
    15001 gcgcagacat ggttgctccg tctggcgaag gcgcagtgcg ttgcatgaag atggcgatgc
    15061 acggtgttga taccccaatc gactacctga actcccacgg tacttcgact ccggttggcg
    15121 acgtgaaaga gctggctgct atccgtgaag tgttcggcga taagagcccg gcgatttctg
    15181 caaccaaagc aatgaccggt cactctctgg gcgctgctgg cgtacaggaa gctatctact
    15241 ctctgctgat gctggaacac ggctttatcg ccccgagcat caacattgaa gagctggacg
    15301 agcaggctgc gggtctgaac atcgtgaccg aaacgaccga tcgcgaactg accaccgtta
    15361 tgtctaacag cttcggcttc ggcggcacca acgccacgct ggtaatgcgc aagctgaaag
    15421 attaattcgg agtaggtcgg ataggacgcg tcagcgtcgc atccgacgat atgcggcaat
    15481 gctgcctccg gtactaaaga ataagggaac cctatggttc cctttttcac atcattgaca
    15541 atcgccgcct gtaccaggca aacttcccgc tttgtcgatt tccttctgaa aagacgtacg
    15601 cgttaattcc tgccaacgca ccgtaaccct gaaaccagag agatgagacg gggatactcc
    15661 tcgccttgcg ctgcattctg gagtaatgca tgactgctgt aagccaaacc gaaacacgat
    15721 cttctgccaa tttttcgctc ttccgcatcg cttttgcggt ttttctcacc tacatgaccg
    15781 tagggttgcc gttgccggtt atcccgctgt ttgttcatca tgaactgggc tatggcaaca
    15841 ctatggtcgg cattgccgtc gggattcagt ttctggctac ggtgctgacg cgtggttacg
    15901 ccgggcgact ggccgatcaa tatggcgcaa aacgttcggc gcttcagggg atgttagctt
    15961 gtggtctggc ggggggcgcg ttgctgctgg cggcgatttt gcccgtcccc gcaccgttca
    16021 aatttgccct gttggtcgtc gggcgtttga ttcttggctt tggtgaaagc cagttactga
    16081 cgggcgctct gacctgggga ttaggcatcg ttgggccaaa acattccggc aaagtgatgt
    16141 catggaacgg aatggcgatt tacggtgccc tcgctgttgg tgcgccgctt ggcctattga
    16201 ttcatagcca ttacggtttt gccgcactgg cgctcaccac aatggcatta cccttactgg
    16261 cgtgggcctg taacggcaca gtgcgcaaag taccggccct ggcgggggaa cgtccatcgc
    16321 tgtggagcgt tgtcgggctt atctggaaac cagggttagg tctggcacta caaggcgttg
    16381 gttttgcggt tatcgggact ttcgtttcgc tctactttgc cagcaaagga tgggcgatgg
    16441 cgggctttac gcttaccgcg tttggcggcg catttgtcgt gatgcgcgtc atgtttggct
    16501 ggatgccgga ccgttttggc ggcgtgaaag tggcgatcgt ctctctgctt gtagaaacgg
    16561 tgggcttgtt gctgctctgg caagccccag gtgcatgggt cgcattagcg ggcgcggcgt
    16621 taaccggagc cggatgttcg cttatctttc ctgcgctggg cgtggaggtg gttaaacgcg
    16681 taccgtcaca cgttcgcggc accgcactgg gcggttacgc cgcgtttcag gatatcgccc
    16741 tcggcgtctc cgggccgctt gcgggaatgc tggcgaccac gtttggttac tcttcggtat
    16801 ttcttgccgg ggcgatctct gcggtgctgg gaattattgt cacgatactg tcatttcgtc
    16861 ggggttaacg aaccagccag accagcatca ggataatcgc gactaacagt atccacagcg
    16921 cgggacgcgt tgccatattt tgcaacgtgt ccatcatcgg tggaaaagga ttaagcagcg
    16981 gttgcatatc acgcggatca atccctttaa cccgccgttg ataaagctga ttaagaatat
    17041 cctcggcctg ggcagaagaa agcgatgatt gcgcagaaag gccatatttt tgctggatat
    17101 atgccgataa cgccgccagt tcactggcat ctaaaggttg tttcagcgcc atctgtagtg
    17161 attcaagcgt cggcgtattt tgctggctta gcgtctgacg cgcctgcagc caggtcacca
    17221 gatggttaaa cagtttcgct ggaattaact cgccatcttt caccccggaa agttccagca
    17281 tcgattgcca gatctgtttg ctgggttctc ccgttgccgc cgcaagtttg gtcaccagct
    17341 gtttgagcgc attgtgctcc gccggtaata acggacggtc ggtcgcctcg cgctgctgcg
    17401 gttgcggaat aaccatcttc ccttcctgca acagggttag aatggttttt aattgctccg
    17461 gtgagagctg attcagcggc gtctgaccaa agttatgacg gatataatcc gtgaccgcct
    17521 gacgattatt ccccagacgt aaatactccc ctaattgcgc taaaagctgg cgggcagaat
    17581 ggcttttttg cgcggccagc agacgttgcg ccagattgtg ctcagcggca gggaagtgac
    17641 gcgaaagcag cggtgaatct cccgacagac caatatcgtg cctgataccc gcccacagtt
    17701 ctgctctttg ctgttgcgtc agtgaggtca ctttcgtcat taagctttcc agcgaagtac
    17761 gttgctgact ggataaaggc tgattgcccg cgccagacgg caggttatct ccctgacctg
    17821 gtggttgccc aggaggaggg ccggaaatag gttgtatcat tacgtatcct tatacctgaa
    17881 atcttcgcaa gtatgcctgg ccgcgagatt atggcacact tgtccggtta actc
//
LOCUS       DAZGQL010000097        17720 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.097, whole genome shotgun sequence.
ACCESSION   DAZGQL010000097 DAZGQL010000000
VERSION     DAZGQL010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17720
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.097"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            132..473
                     /locus_tag="ACHKIR_003957"
     CDS             132..473
                     /locus_tag="ACHKIR_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001720563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RAQPRD family integrative conjugative element
                     protein"
                     /protein_id="HFO9991634.1"
                     /translation="MFSFPASPCRAGRLAGAAGMLFLLSFALPAQAAEKDELATVMRQ
                     LDHVQAALERARTASLQASSQGERYFFDYLRARADLQTIRDGIDHYLAPSRAQPRSSA
                     AVTGDYRRERP"
     gene            473..715
                     /locus_tag="ACHKIR_003958"
     CDS             473..715
                     /locus_tag="ACHKIR_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03758 family integrating conjugative element
                     protein"
                     /protein_id="HFO9991635.1"
                     /translation="MAMTALQNTAFRAASGEVDLSVLHLITLGFLLAVVFLWAAWSLT
                     DAWAGWRSGDVRDGVFGWFMFRLVLLLICSIWMFAG"
     gene            792..1106
                     /locus_tag="ACHKIR_003959"
     CDS             792..1106
                     /locus_tag="ACHKIR_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03745 family integrating conjugative element
                     membrane protein"
                     /protein_id="HFO9991636.1"
                     /translation="MAVFLCTGITPALADLPQIEPPTSGGGGGLFGQIKGYLQDGVVL
                     FGLVLSAVAFVKVAEAALSTFSEVRDGKAGWGQFGAIVVVGVVLLVAIIWLLGKSASI
                     IL"
     gene            1119..1481
                     /locus_tag="ACHKIR_003960"
     CDS             1119..1481
                     /locus_tag="ACHKIR_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03750 family conjugal transfer protein"
                     /protein_id="HFO9991637.1"
                     /translation="MQTIRFLPDRLNVEPAVFRGFTTPELGLAALTGAVAGGIWPLPL
                     IPLLGWVIIPTGMLLTPLLLVWFGGVWISRLKRGKPDNWLWQHLAKKSSAWGGGRKTF
                     IREAHGWSLRRTRSRGKK"
     gene            1478..2140
                     /locus_tag="ACHKIR_003961"
     CDS             1478..2140
                     /locus_tag="ACHKIR_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PFL_4703 family integrating conjugative element
                     protein"
                     /protein_id="HFO9991638.1"
                     /translation="MSRFRHALRDRDQHILTLRIFSGVLAGILMLVCIGWMTAPDRLT
                     IHNPPDLRSGSTRPWWEVPPSTVYSFAFYIFQQLNAWPKNGEVDYPAKIHALSAYLTP
                     SCREILESDARRRSDAGELLERVRVVYEIPEHGYGGRYNKNPVTVEGRDNWQVQLDLV
                     ADEYFHAEPVKRALVRYPIKVVRMEGDPERNPFGLALDCYSSTPQRLEAASPAAGKGD
                     NQ"
     gene            2140..3048
                     /locus_tag="ACHKIR_003962"
     CDS             2140..3048
                     /locus_tag="ACHKIR_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03749 family integrating conjugative element
                     protein"
                     /protein_id="HFO9991639.1"
                     /translation="MNGAERCLYRSIVMVLSLLVLPLSARADELMKWERIPLPVTLMA
                     GQERVIFADKNVRVGFPPALNGKLRVQSTGGAVYLKAESVFPQTRLQLQDVESGEIIL
                     LDVTAAEKGPTEPVRIVYEGSVSTVSQAGTAASARSAEHKKVVPGGDDGSQALRQPVK
                     YAEPLPVLLTRYAAQSLYAPLRTLEAVPGIRPVNTHLPGKITTLYPSEPLTVTPLAAW
                     AAGGRAVVALKLTSTVSRKIILDPRALQGKFIAATFQHRWLGAAGTPEDTTTLYVVTA
                     ERPEQAFIAEPASRRIPSRGEVKHAD"
     gene            3038..4525
                     /locus_tag="ACHKIR_003963"
     CDS             3038..4525
                     /locus_tag="ACHKIR_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001506261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03752 family integrating conjugative element
                     protein"
                     /protein_id="HFO9991640.1"
                     /translation="MQIKSNTIVKYVVPCVVVAGILTGLKSCGGDTGEPSSAESSGTL
                     AGLSPDELKALGVEGDTPEDTLRTLIGRMNQVRERQLVLDKQNEKLLKENEMLRKRGT
                     DVSGQVSEAVAGLQKNYEQSQRQFRNEQNALMTQVQSLTEQLKRQQESADAGAGGQES
                     DIPAGLGLDGLGSVTGSAASPGTDGLMWVTPADQVQDEASSVGDKSGPRFPVSFLDDN
                     PLTRQKAAYEQQVKGYSNEQQKASTRAVYTLPENSTLAGSRAMTALLGRVPVNGTVTD
                     PYPFKILVGKDNLTANGIELPDVEGAVVSGTASGDWTLSCVRGQVNSITFVFADGTVR
                     TLPSSDVNGSSNAQAKNNSSTGGIGWISDDDGIPCISGTRKSNASTYLPTIFALSAAG
                     AAGDALSEGQYTSQNNVNGITSTLTGSAGEAALGKALSGGMNETAEWVKQRYGQTFDA
                     IYVPPGQTLAVHITRQLAIDYEDQGRRVRYDFTLPGEVHGTGGLD"
     gene            4561..4953
                     /locus_tag="ACHKIR_003964"
     CDS             4561..4953
                     /locus_tag="ACHKIR_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004115530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03751 family conjugal transfer lipoprotein"
                     /protein_id="HFO9991641.1"
                     /translation="MTVMVAVFILPGCSTSKEELLPPGEQSMLEIWQGKGSGGHSRHM
                     TAARDTLRRPLTMPEQQADAHGAISYSRTQESEISQQFPRLPNPDMVMYVFPHLASGT
                     SPVPGYSTVFPFYSRTQYAMPGERTEAL"
     gene            5091..7787
                     /locus_tag="ACHKIR_003965"
     CDS             5091..7787
                     /locus_tag="ACHKIR_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015675344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer ATPase"
                     /protein_id="HFO9991642.1"
                     /translation="MTRQDETKVYHACPSVIDFLPWVEYLDEEQCLLLDDGVSVGAVY
                     EVSPAATEGRTAERLEQIRDTVEDALQDSFDEYDSHPWVVQFFCQDENDVDTYVDQLR
                     GYVKPHAEGSSFTEAWLREMERHLKGIARPEGLFRDTLVTGQPWRGQQRRTRMVIYRW
                     IGKNNHDPMPPVAMLNQVCSRVVGALGGAGVRCTRMNGQQVHGWLLRLFNPRPEWVDR
                     DILYRMASRAEPQETPEGMMPVMTDFAESLWFTPPVSDPENGVWWLDGLPHAAVVVEK
                     LRTPPEPGTITGEQARGEKTVNALMDTFPEGTVLCMTIVVQPQDTLEERFTRLSKNAV
                     GENSESLRVREDVATVKKYLGNRHKLYRAGITFLLRAEDMESLKRRRVELTTVLLGAG
                     LQPVRPEFDVGPLNSWLRALPMCFDPDTDKKQWYTRLMWVQHLAGLLPVTGRETGTGH
                     PGFSFFNRGGDVLTFDPLNKLDRTQNAHLLLFGPTGAGKSATLCGSLSQIMAVHRPRL
                     FIAEAGNSFGLLADYFESLGLSVNKISVKPGTGVCLPPFADAHQLVEQGETLQSVDEH
                     SLPDLDEDEGDEEEEKRDILGEMEISARMMITGGDPKEEAALKRADRAMIREALLMAT
                     HTTYREGRQMLPVDLQSALWEISRDTQRNDVRRAKAAEMAESLGMFTQPGSFEAELFN
                     REGKLWPEADVTLIDLGHLAREGYEAQMALTMVSMTNMINNIAERDQYLGRDILFVVD
                     EAHIVTVNPLLSPYMTKVVKMWRKLGAWLWLATQNLKDYPDVSEKMLNMAEWWVCLTM
                     PPEEVNNIARFKSLTEEQKAVLLSASKLSGCYTEGVVLAKRTEALFRVVPPSLYLALG
                     MTEKEEKAERRALMKVHQCSELEAAFHVARKLDGLRGLAGRE"
     gene            7792..8169
                     /locus_tag="ACHKIR_003966"
     CDS             7792..8169
                     /locus_tag="ACHKIR_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFO9991643.1"
                     /translation="MLCLKYPEPEVVSAVHPAGSVFVLPPQGAPGISCTTRDNLERLR
                     GHLAAQLGRVECIRCQPQRVGLNSSVAVMLEGQQGQYVHILLTVSGHESWPSEEEYIH
                     PRWYISVTDAADLFYLLLWLGEG"
     gene            8547..8735
                     /locus_tag="ACHKIR_003967"
     CDS             8547..8735
                     /locus_tag="ACHKIR_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HFO9991644.1"
                     /translation="MGKRYKITATIIKPGNMPVQWLRYQDEKMTQAECEKLFAPAKEP
                     GRYIGVRVEDFRCEVVKA"
     gene            9121..9570
                     /locus_tag="ACHKIR_003968"
     CDS             9121..9570
                     /locus_tag="ACHKIR_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000914970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9991645.1"
                     /translation="MLKKTILALCISAPFLVAGCDDATKENVSKKAADIKEAAQTQLS
                     DVKDAAEDVKMQAAGKVAEVSEAAQSGLSEVKDAVEEVKTQAAGKMAEVSEAAQSGLS
                     EVKDAVEEVKTQAAGKVAEVSEATQSGLSDVKDTAPEKGAEISVTEE"
     gene            9762..10394
                     /locus_tag="ACHKIR_003969"
     CDS             9762..10394
                     /locus_tag="ACHKIR_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFO9991646.1"
                     /translation="MLLIAGHLRPAFQAHTGRFLIADLSEEVVRRYLRFCTPLSGMAV
                     SPSSLLLQIRPQMSVLAECLIRQLITDEPSPVYFRDAVAFSSLALFEEHPDFHALLTG
                     CIRSCTVRVKNIISADLSRPWMLGEVASLLYISESLLKKKLQEELTTFSELLLKERMQ
                     YAAQLLSAGENRISRVAELCGYASASYFISVFRRYYGITPNQWLPVMQPD"
     gene            10530..10925
                     /locus_tag="ACHKIR_003970"
     CDS             10530..10925
                     /locus_tag="ACHKIR_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03757 family integrating conjugative element
                     protein"
                     /protein_id="HFO9991647.1"
                     /translation="MYRLLLYSFLILPVAASAGTTIYTDSHQRPMNPPAGVRVVLLDA
                     PEQTQDTFFGVLPAEPAEASARVMERMQSPEWQSFQAGLAEHYRALAHAWSLGLKKYP
                     AVVFDDSEVVYGTTDVVLAEQLRTGGGQP"
     gene            10922..11896
                     /locus_tag="ACHKIR_003971"
     CDS             10922..11896
                     /locus_tag="ACHKIR_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03756 family integrating conjugative element
                     protein"
                     /protein_id="HFO9991648.1"
                     /translation="MISCTSRHRAGVLLLSMAVLPVAEASLNTAQIVASAASQSCISW
                     RVSGICYWLFCTKFSCTVRTSVKVSHYIPEVVVSTYTAPGGNPWREMSAVSSTAGGPE
                     NAISKGIAGLSAGGGNPADMKVQGERRSSIRFKYADATGHPATTLIGGMVPGYSCESP
                     VTPFVPYFLSTLDSIAWRTGLPESLYPEALIPGKREIGSQEEENMWGNVYPRSGFVTQ
                     TDDDKAAAVVAQRVADIITRTGEPHVYQPVKAGKKDGYWPPDPVEENTGTRNHKWQRL
                     TPSVSTSCAVFPDGEHEAAETGNAAFALWQPYSCCKKRGQTFLGSTNF"
     gene            11910..13331
                     /locus_tag="ACHKIR_003972"
     CDS             11910..13331
                     /locus_tag="ACHKIR_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001506259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrating conjugative element protein"
                     /protein_id="HFO9991649.1"
                     /translation="MKQKMTIPVLAVVLCAGTVQADGNFLSLPKVNDSAFGYGKSVDG
                     AVSDKLFYTLGGGSVISQPATRSNMQKLGVDLGWSSDLMCGNFDLKTTVGNQLNGITD
                     GFKNLMGDVIQGASGAVASLPAMVIQRANPGLYDMLTNGVLQANVAFDKAQLNCQNMA
                     KRMMEFTDSNKWTQAAMLEEYKKIVNTGDGDAIKGDEAGRQVTGVEGQRWIGGQQRGG
                     AGQSAIRPTHDLTAAGFNMMNSLPSTSTSSVSPGNCNGSACMKYRNAEEAARAVVKVL
                     GDKSMRTCGDAKACTSGDADQQPGTSIAGTGFAPMLEEATRVNTELLAGMVNGRDKPT
                     AGNLAKLKAGSLSVTAGVIRALQRDPDNGALTSRLAGELAMADTVETALLMRRMLLTG
                     MAEPYAAAQPAALEEGERRIEALDREIMALKNEMELKRDLARNSVLTIIERDNERVGN
                     NPMIQQADNADSRIHSLDAPDKE"
     gene            13336..13677
                     /locus_tag="ACHKIR_003973"
     CDS             13336..13677
                     /locus_tag="ACHKIR_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9991650.1"
                     /translation="MSRIYGRVKKRMKVFALYTLLLTGITVFSLWLADTGIRHAEGFA
                     ALREWMYETRYGWLGWRLCLYSAIGWLGWRIWHAPGFRAEYRASFLRMATVSVLFILL
                     CEYAIFAEPGR"
     gene            13684..15180
                     /locus_tag="ACHKIR_003974"
     CDS             13684..15180
                     /locus_tag="ACHKIR_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG N-terminal
                     domain-containing protein"
                     /protein_id="HFO9991651.1"
                     /translation="MTTNNYLEYFLTLLGWVVNNGLWNVLISTGLFALPLAFRIIGIW
                     LKVREEGADEGNKGMLSLPRIEHVLYGAFFVMISCCVPLVQVTLDTLSFDKSRAQTCG
                     VWTPEEPGETGYSGVISSIGDQTAAAPVWWVVVHKLSKGVTQAAVASIPCRPDLRQLR
                     FEIQRTFIENRALADELQDFTNDCYSLALYMWKQRDQGQSKDKKVLDDISWIGSATFM
                     QGDYGTLQSKMPRAAFPWNTARDSGRPDTGRGGYPTCREWWSDSSVGLKKRVMEEVSD
                     HYWLRLSAAMKMIGSSQVDYKEEVIKRLVSPANLTLSQGGEVYRGYGGNADMTTMNAV
                     ARIAGTAGSAVAALGAFPALDAMRQALPMVQSIILMAIYIMIPLILAFAAYELSTLIT
                     LTFVVFAVHFLTFWWELARWLDSWMLTALYSSDTHTRFNMMGFQNTSDDLIMNLVMGT
                     MFLVLPAVWLGALSWAGVSVGTVMGDAINKGTSNVSKAAAKSGDLANK"
     gene            complement(15231..15470)
                     /locus_tag="ACHKIR_003975"
     CDS             complement(15231..15470)
                     /locus_tag="ACHKIR_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001058544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9991652.1"
                     /translation="MNNESEKAMWRCKPVDNPWTFTSLLKKAVCAVLPDFTPAQDKPQ
                     NMFSLSNEKDTDYSVPMRPFALYDPWKHHHDNADF"
     gene            15660..15923
                     /locus_tag="ACHKIR_003976"
     CDS             15660..15923
                     /locus_tag="ACHKIR_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFO9991653.1"
                     /translation="MKPNIHPEYRTVVFHDTSVDEYFKIGSTIKTDREIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVGTQKGE"
     gene            complement(15969..16144)
                     /locus_tag="ACHKIR_003977"
                     /pseudo
     CDS             complement(15969..16144)
                     /locus_tag="ACHKIR_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976766.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            16336..16908
                     /locus_tag="ACHKIR_003978"
     CDS             16336..16908
                     /locus_tag="ACHKIR_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9991654.1"
                     /translation="MLTKKMLLVCASCVALSACSAQEIAELNRQISDGAFAVTKVLNV
                     GVNQADDDLKYLSQAPRAKREKRDHVIEVPVDVDTAAGRLKRYYKFYSNEEVDAIRNN
                     GHANGKWAAAAISQNGHMWDAMPGSYYKMGSDWDGGEFDDHLTLELEKNGKGSRIYIT
                     YASPAPGHLKDKFIEPLVKRVKDVAEGRVR"
     gene            complement(17057..17632)
                     /locus_tag="ACHKIR_003979"
     CDS             complement(17057..17632)
                     /locus_tag="ACHKIR_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HFO9991655.1"
                     /translation="MDFSTLITFSIITLSQTVSIGPGVALIISESLTYGAKKAITTSI
                     YIRFGEIVVMLVTLFSIALAINLEKHLDTIKTCGGGYLIFLGITRLIKRKGSKGNKAN
                     IPFLIPMLNPKAFLFFAAFMPSFIKNNSNFNTDFLLLCIIFIIVSLLTDLIYICICLT
                     IRGRINSSFSKYVNFISSLFILLTGAYLVFL"
BASE COUNT         4148 a         3920 c         5484 g         4168 t
ORIGIN      
        1 gttgtttgcc ttcatccaca ccagtgtatt ctcccgtaaa aattttctgt tttgcaaata
       61 gagtttatcg ctgatccagt cctctgcctt acgcatcctg tacgcactgt ttaactctga
      121 cggaggtgcg tatgttttct tttccggcat caccctgccg tgccggccgt ctggccggtg
      181 ccgcggggat gctgtttctt ttgtcctttg cattacctgc gcaggctgcc gaaaaggatg
      241 aactggccac tgtaatgcgc cagcttgacc acgtgcaggc agcgctggag cgggccagaa
      301 cggcctctct gcaggcttct tcacaggggg aacgctactt ttttgattat ctgcgtgccc
      361 gtgcagatct gcagaccatt cgtgacggta ttgaccatta tctggcccct tcccgggcgc
      421 aaccccggtc ttccgcagcc gtgaccggtg actaccggcg ggagcgtccc tgatggcgat
      481 gacggccctg cagaatacgg ccttccgggc tgcttcaggt gaagtggatc tcagtgtgct
      541 gcatctcatc acgctgggat ttctgctggc agttgtgttc ctctgggcag cgtggagtct
      601 gacggatgcc tgggcaggct ggcgtagcgg tgatgtccgg gatggtgtgt tcggctggtt
      661 tatgttccga ctcgtcctgc tgctgatctg cagtatctgg atgtttgcgg gttgatttca
      721 gtgtcttgtt taagtttcag gaggaaataa tgcataaaaa aatgcgcatc tgtcgccagg
      781 cctggtatgc catggcggtg tttctgtgta ccgggatcac acctgctctg gctgatttgc
      841 ctcagataga gccgccaaca tccggcggag gtggtggact tttcggacag atcaaaggct
      901 atctccagga tggtgttgtg ctgtttggtc ttgtgctcag tgcagttgct tttgtgaagg
      961 tggcggaagc ggcgctttcc accttctcag aggttcgtga cgggaaggcc ggatgggggc
     1021 agtttggtgc catcgtggtg gttggtgttg ttctgctggt cgccattatc tggctgctgg
     1081 gcaagtcggc ctccatcatt ctgtaaggag acatccccat gcagaccatt cgttttctgc
     1141 ccgatcgtct gaacgtggag cctgcggttt tccgtggttt taccacaccg gaactggggc
     1201 tggcggccct gacaggcgct gtggcagggg ggatctggcc gttaccgctg attcctctac
     1261 ttggctgggt gattatcccg acagggatgt tgctgactcc cctgctgctg gtctggttcg
     1321 gtggcgtctg gatcagccgg ctgaagcggg gaaaacctga caactggctc tggcagcatc
     1381 tggcgaagaa aagcagtgca tggggtggtg gccggaaaac atttatccgg gaggcgcatg
     1441 gctggtccct gcgccgtacc cgtagcaggg ggaaaaaatg agtcgttttc gtcatgctct
     1501 gcgggatcgc gaccagcata tcctgacact gcgtattttc agcggtgtgc tggccggaat
     1561 tctgatgctg gtctgtatcg ggtggatgac ggcaccggac agactgacaa ttcacaatcc
     1621 gccggatctg cgttccggca gtacccgtcc ctggtgggaa gtcccgccgt caacggttta
     1681 ttcctttgcc ttttatatct ttcagcaact gaatgcctgg ccgaagaacg gagaggtgga
     1741 ctacccggcg aaaattcatg cgctctctgc ctacctgacg ccatcctgtc gggagattct
     1801 ggagagtgat gcccgccgtc gcagtgatgc gggggagctg ctggagcgtg tgcgcgtggt
     1861 gtatgaaatt ccggaacatg gctacggggg caggtacaac aaaaatccgg tgacggtgga
     1921 agggcgggat aactggcagg tacagctgga tctggtggca gatgagtatt ttcatgctga
     1981 gccggtaaaa cgggcacttg tccgttatcc gataaaggtg gtgcgcatgg agggggatcc
     2041 tgaacgtaat ccgttcgggc tggcgctgga ctgctacagc agtacaccac agcggctgga
     2101 agcagcctcc ccggcagccg ggaaggggga taatcagtga tgaacggagc tgaacgttgt
     2161 ttataccgta gtattgtgat ggtcctgtca ctgctggttt taccactgag cgcacgggct
     2221 gatgagctga tgaaatggga gcgcattccc ttacccgtta ccctgatggc aggacaggag
     2281 cgggttattt ttgcagataa aaatgtgcgg gtgggcttcc cgccagcact gaacggaaag
     2341 ttgagggtac agagtaccgg tggggcagtc tatctgaaag cagagagtgt ttttccgcag
     2401 acgcgtctgc aactgcagga tgtggagagc ggagaaatta ttctgctgga tgtgacagca
     2461 gcagaaaaag ggcccacaga gcccgtacgg attgtgtatg agggaagcgt cagtacggtc
     2521 agtcaggctg gcacagcggc gtctgcacgc tctgcagaac acaaaaaggt ggtacccggc
     2581 ggggatgacg gctcgcaggc cctccgtcag ccggtgaagt atgcagagcc tttacccgtt
     2641 ctgttgaccc gctatgcggc acagtcgctg tacgcaccgt tacggacgct ggaagcggta
     2701 ccgggtatcc ggccggttaa tacacatctg cccgggaaaa tcaccacact ctatccgtca
     2761 gagccactga cagtcacgcc actggcagcc tgggctgcgg gtgggcgtgc agttgttgca
     2821 ctgaaactga cgagtacggt aagccgcaaa attatccttg atccgcgggc acttcaggga
     2881 aaattcattg ccgcgacttt ccagcaccgc tggctgggtg cagcaggtac ccctgaggat
     2941 accaccacgc tctatgtggt gacggcagaa cggccggaac aggcgtttat cgcggagcct
     3001 gcctcccgtc gcattccttc acggggggag gtgaagcatg cagattaaat ccaatacgat
     3061 cgtgaagtat gtggtgccct gtgtggtggt ggcggggatc ctgacgggat taaaaagctg
     3121 tggaggagat accggggagc cctcgtctgc ggaaagctca ggaacgctgg ccggactgtc
     3181 accggatgaa ctgaaggccc tgggcgtgga aggagacaca cctgaagata ccctgcgcac
     3241 actgattggg cgtatgaatc aggtgaggga gcggcaactg gtactggaca aacagaatga
     3301 aaagttgctg aaagaaaatg aaatgctgcg caagcggggc acggatgttt ccggtcaggt
     3361 cagtgaggca gtggcaggat tgcagaaaaa ttatgagcag tcgcagcgcc agttccggaa
     3421 tgaacagaac gcgttaatga cacaggttca gtcactgaca gagcaactga agcgtcagca
     3481 ggagagtgct gatgccggcg ccggaggaca ggagagcgat attccagcag gtctgggcct
     3541 tgacggactg gggagcgtga caggatcggc agcctcaccg ggaacggatg gtctgatgtg
     3601 ggtgacaccg gccgatcagg tgcaggatga agcgtcgtcg gtgggggata agagcggacc
     3661 gcgttttcca gtctcttttc tggatgacaa tccactgacc cgtcagaaag cggcttatga
     3721 acagcaggtg aagggataca gcaatgaaca gcagaaggcg agcacccggg cagtgtatac
     3781 cctgccggag aactcgacgc tggcaggcag ccgggcgatg acagcgcttc tgggacgggt
     3841 tccggtgaac ggaaccgtga cggatcccta tccgttcaag atcctggtgg gtaaggataa
     3901 cctgacggca aacggaatag agctgccgga tgtggaaggt gcagtggtgt ccggtactgc
     3961 gagtggtgac tggacgctct cctgtgtccg tgggcaggtg aacagcatca cgtttgtttt
     4021 tgccgacggg acagtgcgta cgctgccgtc atcagacgtg aacggcagca gtaacgcaca
     4081 ggcaaaaaat aacagcagta caggaggcat tggctggatt tcggatgatg acgggatccc
     4141 gtgtatcagc ggaacccgta aatccaatgc ctccacgtat ctgccgacaa tatttgccct
     4201 gtctgcagcc ggcgctgcgg gtgatgcact gagtgagggg cagtacacct cacagaacaa
     4261 cgtcaatggc atcacatcaa cactgaccgg cagtgccgga gaggcggcac ttggaaaagc
     4321 cctgtccggc gggatgaatg aaaccgcgga atgggtgaag cagcgatacg ggcagacgtt
     4381 tgatgccatt tatgttcccc cggggcagac gctggcggtg catatcacgc gtcaactggc
     4441 cattgattat gaagatcagg ggcgccgggt acgttacgac ttcactctgc cgggggaagt
     4501 ccacggtacc ggtggtctgg actgatgtaa ggagatcccg tgaaacgaca gttatctgta
     4561 atgaccgtca tggtggcggt atttattctg cccggctgct cgacatcgaa agaggagctt
     4621 ctgcctccgg gtgaacagag tatgctggag atctggcagg ggaaaggaag cggaggacat
     4681 tcgcggcaca tgacagcggc cagggacacc ctgcggcgtc cgctgacaat gccggagcag
     4741 caggcagatg cgcacggagc cataagctac agccggacac aggaaagtga gatcagtcag
     4801 cagtttccgc ggctgcccaa tcctgacatg gtgatgtatg tttttccgca tctggcttcc
     4861 ggtacgtcac cggttccggg gtacagcaca gtatttcctt tttacagccg gacgcagtat
     4921 gccatgccgg gtgaacgaac ggaggcgttg tgatgcgatt ttccctgttt acagacacaa
     4981 aaaaacgggt ttcttccccg gtgtcgcagc atgacggaga agcggtatct ccggatatga
     5041 aagaaagagt ggcccctcag gtacgaaagg cactgtcaag gccggggaag atgacccgac
     5101 aggatgagac gaaggtttac catgcctgtc cgtcggtgat tgattttctg ccatgggtgg
     5161 aatatctgga cgaagagcag tgcctgctgc tggatgatgg ggtgtcggtt ggtgccgtgt
     5221 atgaggtgag tccggccgct acggaagggc ggactgcaga gcgtctggag caaatccggg
     5281 atacggtgga ggatgcgctg caggacagtt ttgatgaata tgactcacat ccctgggtgg
     5341 tgcagttctt ctgtcaggat gaaaacgacg tggacacgta tgttgaccag ctcagggggt
     5401 acgtgaaacc tcatgcagaa ggctcctcat ttacggaggc ctggctgagg gaaatggagc
     5461 gccatctgaa ggggattgcc cgaccggaag gtctgttcag ggatacgctg gtcacaggtc
     5521 agccatggcg tggtcagcag cggcgcacga ggatggtgat ttaccggtgg atcgggaaaa
     5581 acaaccacga tccgatgccg ccggtggcaa tgctgaacca ggtctgttcg cgggtcgtgg
     5641 gggcgctggg tggcgcaggc gtccgctgta cacggatgaa cgggcagcag gtgcatggct
     5701 ggctgctgag gctgttcaat ccccggccgg aatgggtgga cagggatatt ctgtaccgta
     5761 tggcatcccg tgcagaaccg caggagacac cggagggaat gatgccggtg atgaccgatt
     5821 ttgcagaaag cctctggttc accccaccgg tatctgatcc ggaaaacggc gtgtggtggc
     5881 tggacgggct tccgcatgcc gctgttgtgg tggagaaact gcgcacacca ccggagccgg
     5941 gaaccatcac aggggaacag gcccggggcg aaaagacagt gaatgccctg atggacacct
     6001 ttccggaggg aacggtgctg tgtatgacaa ttgtggtcca gccacaggac acactggaag
     6061 agcgcttcac gaggttgtcg aaaaatgcgg tcggggagaa cagtgaatcc ctgcgtgtcc
     6121 gggaggatgt ggcgaccgtg aagaaatatc tgggaaaccg gcacaagctc taccgtgccg
     6181 ggatcacgtt tctgctgcgg gcagaggata tggagagtct gaagcgcaga cgggttgaac
     6241 tgacgacggt gctgctgggc gcgggcttac agccagtgcg cccggagttt gatgtcggac
     6301 cgctgaacag ctggctgcgc gcgctgccca tgtgttttga tcctgatacg gataaaaaac
     6361 agtggtacac gcgtctgatg tgggtacagc atctggcagg gctcctgccg gtgacgggaa
     6421 gggaaaccgg tacggggcat cccggattca gtttctttaa ccgtggcggg gatgtgctga
     6481 cctttgatcc gctgaacaag ctggaccgga cccagaatgc acatctgctg cttttcgggc
     6541 ctaccggagc cggtaaatcg gccacgctgt gtggttcgtt gtcgcagata atggcagttc
     6601 atcgtccccg tctgtttatc gctgaggccg ggaactcgtt tggtctgctg gcggattatt
     6661 ttgagagtct cgggctttcg gttaataaaa tcagtgtcaa accgggaacg ggggtgtgtc
     6721 ttcctccatt tgcggatgct catcaactgg tggagcaggg agagacgctg cagagtgtgg
     6781 atgagcattc actgcccgat cttgatgaag atgaggggga tgaagaggaa gagaaacgcg
     6841 acattctggg ggagatggaa atctctgccc gcatgatgat caccggaggg gatccgaaag
     6901 aagaagccgc gctcaaacgt gcggacaggg cgatgatccg tgaggcactg ctgatggcca
     6961 cgcacaccac gtaccgggag gggcgtcaga tgctgccggt ggatctgcag agtgcgttgt
     7021 gggaaatttc gcgggacact cagcgtaatg atgttcgccg ggctaaagct gctgagatgg
     7081 cagagtcgct ggggatgttc acccagccgg gcagttttga agcggagctg tttaaccggg
     7141 aaggaaaact gtggccggaa gcggatgtga ccctgattga tctgggacat cttgcccgtg
     7201 aaggttatga agcccagatg gcactgacca tggtgtcaat gactaatatg attaacaaca
     7261 ttgccgagcg cgatcagtat ctgggacggg atattctgtt tgtggtggat gaggcgcata
     7321 tcgtgacggt aaacccgctt ttgtccccgt atatgaccaa agtggtgaaa atgtggcgca
     7381 agctgggggc ctggctgtgg ctggccacgc agaacctgaa ggattatccg gatgtctcgg
     7441 agaagatgct gaatatggca gagtggtggg tgtgcctgac catgccgccg gaggaagtga
     7501 acaatattgc ccgttttaaa tccctgacgg aggagcagaa ggcggtactg ctgtcagcca
     7561 gtaaactgtc cggctgttat acggaggggg tggtactggc gaagcgcaca gaagcgttgt
     7621 tccgggttgt gccaccgagt ctgtatctgg ctctggggat gacggagaaa gaagagaagg
     7681 ctgaacgccg cgccctgatg aaggtgcacc agtgcagcga actggaagca gcgtttcatg
     7741 tggcacgaaa gctggacggg ctacgtggtc tggctggcag ggagtgagac tatgctgtgt
     7801 ctgaagtatc cggaaccgga agtggtgtct gctgttcacc ccgccggaag tgtgtttgtt
     7861 ctgccgccac agggtgcgcc ggggataagt tgcaccacac gggataatct tgaacgtctt
     7921 cgcgggcatc tggcggcgca actggggcgg gtggagtgta tccgttgcca gccacagagg
     7981 gtgggtctga acagcagtgt ggcggtgatg ctggaaggtc agcagggtca gtatgtgcat
     8041 attctgctta ctgtgagtgg gcatgagagc tggccgtcgg aggaggagta tattcatccc
     8101 cgctggtata tatcagtgac tgatgcggcg gatttgttct atctgctgtt gtggctggga
     8161 gaggggtgat atgaggagcg atatcgattt tccgggacca ctgtatccat cgctgtcaga
     8221 tcaaattgcc agtgaacctg tgctgtttct ggcgggagct gtgggtgtgg ctgtgttttc
     8281 atgggtgctt gcgaatgtac tggcccggat gctgtcaggg ttgtactccc gtgtcaggag
     8341 gagggtaaag aactctgaaa aatagcggga gagaggtgct gccctgatgc cttcgttgta
     8401 ttctgcgacg ctgttggtgc ccgtattttg tgcgggtacc gtacagaatc accgcatgag
     8461 ccattgctga gacgctcact ttttttcgca tgcattaccg gagtgatggc tcagccggtg
     8521 attttgttgt tgagatgaga gccagtatgg gaaagcgata taaaataact gcgacaataa
     8581 tcaaaccagg caatatgccg gttcagtggc tgcgttacca ggatgaaaaa atgactcagg
     8641 ctgaatgtga aaaactgttt gcgcccgcaa aagagccggg acggtatatt ggcgtcaggg
     8701 tggaggactt ccggtgtgaa gtggtgaagg cctgagtatg aaaataaaaa acctccggcg
     8761 gagtcggagg gggcaaaaac agatagtccc ggagtgtatc tgtgtaaatg gtgttattgg
     8821 tataataatg gtggtaaaga ccacgggaga atttattcat tttattcgtc atgaaatcaa
     8881 cggtaatagt gtgtttctgt gacgggaata aaaaaataca gcaccgccga tgacccggtg
     8941 ctgaaaacag gttaaatgta gtgggcacat ttccggtaat atcaggaaca gcatgttatg
     9001 gttataaaag catataccgg gagtgtgccc tgacactgtg atgttctggt tgtcttttga
     9061 ccattttccg gggacgaaat ataagctgtt ctgcgttaac acttaataat gagagattgt
     9121 atgctgaaaa aaacaattct ggcgttatgt atttccgcac catttctggt tgcgggctgt
     9181 gatgatgcga caaaggagaa tgtaagtaag aaggcggcag atataaagga agctgcgcag
     9241 actcaactca gtgatgtgaa ggatgccgcg gaggacgtga aaatgcaggc tgccggaaaa
     9301 gtggcagagg tttctgaagc agctcagtcc gggctcagcg aggtgaagga tgccgtggag
     9361 gaagtgaaaa cacaggcagc cggaaagatg gcagaggtct ctgaagcggc tcagtccggg
     9421 ctcagcgagg tgaaggatgc cgtggaggaa gtaaaaacac aggcagccgg aaaggtggca
     9481 gaggtctctg aagcgactca gtccggtctc agtgatgtga aggataccgc tcctgaaaag
     9541 ggggctgaaa tatccgtaac ggaggagtaa aagagcgccg ccggtgcagt ttttttcagc
     9601 acccggtgac acggaatttt atatacgggg catgtctgtc tctttaataa atcagcatat
     9661 ttaccggata tccgatgccc tgtacctgcg ctgtcattat ggctctcaaa cagacaatgc
     9721 tttgtctgga gcacaggaac tctgtcatac ctgccggcac aatgcttctg attgccgggc
     9781 atctgcgccc tgcttttcag gctcataccg gacgtttcct tatcgctgac ctcagtgagg
     9841 aggtggtgag gcggtatctg cggttctgca cgccattatc cggtatggca gtcagcccgt
     9901 cgtctttgct tttacagatc cggccacaga tgtccgtgct ggcggaatgt ctgatcagac
     9961 aacttattac agatgaacca tcgccggtgt acttcaggga tgcggtggct ttttccagtc
    10021 tggcgctgtt tgaggaacat ccggatttcc acgccctgct cacggggtgt atccggagct
    10081 gcactgtccg ggtgaagaat attatcagtg ctgatttgtc ccgtccatgg atgctgggtg
    10141 aagtggccag tctgttatat ataagtgaga gtttactgaa gaaaaagctt caggaagagc
    10201 tgacgacctt cagtgagctt cttctgaaag agagaatgca gtatgccgct cagcttttgt
    10261 ctgcgggaga aaaccgtatt tcccgggtgg cagagttgtg tggctatgcc agtgcttctt
    10321 attttatttc tgtgttcagg cgttattatg gtattacccc gaatcagtgg cttcctgtga
    10381 tgcaaccgga ttaatttgat tcagatgcag agtgtgcgtt attcagagga agaggcttaa
    10441 tttttatcat ccggttttga atttattgct gattgtccct ttgcctttgc gcacgctgtc
    10501 agataccagt tgtctgacgg agtcgcatca tgtaccgact gttactgtat tccttcctga
    10561 tactaccggt ggctgcatct gccggtacaa caatttatac cgattcgcac cagcgcccta
    10621 tgaacccgcc agcaggtgtt cgtgttgtcc tgcttgatgc tcctgagcag acgcaggata
    10681 catttttcgg ggtacttccc gcagagcctg cagaagcatc tgcccgtgtc atggaacgga
    10741 tgcagtcgcc tgaatggcag tcgtttcagg cagggcttgc tgaacactac cgtgcattag
    10801 cacatgcctg gtcccttgga ctgaaaaaat atccggctgt ggtttttgat gacagtgaag
    10861 tggtctacgg aaccacagac gtggtgctgg cagaacaact gcgtacaggg ggagggcagc
    10921 catgatttcc tgcacatcac gtcatcgcgc cggtgttctg ctgttaagta tggcggtatt
    10981 gccggtggca gaggcatcgc tgaacaccgc gcagatagtg gccagtgcag cctcacagag
    11041 ctgtatcagc tggcgggtca gcgggatttg ctactggctg ttctgcacca aattcagttg
    11101 cacggtcaga acatcagtga aagtttccca ttatatcccg gaagtggttg tttccaccta
    11161 taccgcacct ggcggaaatc cctggcgtga aatgtcggcg gtcagcagta cagccggtgg
    11221 tccggagaat gccataagca aaggtattgc cggtctgtca gcaggaggag ggaaccccgc
    11281 agacatgaag gtacaggggg aacgtcgttc gtcaatccgt tttaaatatg ccgatgcaac
    11341 agggcacccg gccaccacgc ttatcggagg gatggtgccg ggatattcct gtgagtcacc
    11401 ggtgacacct tttgttcctt attttctcag tacgcttgac agtattgcct ggcgtacagg
    11461 actaccggag tcactgtacc cggaggccct gatccccggt aaacgcgaaa taggcagcca
    11521 ggaagaagag aatatgtggg gcaatgtgta tccccgctcc gggttcgtga cccagacaga
    11581 tgatgataaa gctgctgcgg tggtggccca gcgggtggct gacattatca cccgcaccgg
    11641 tgagccacat gtctatcagc cggtgaaagc agggaagaag gacggttact ggccaccgga
    11701 tccggtggag gaaaatacgg gcacccggaa ccataaatgg cagagactga cgccttcagt
    11761 ctccacgtcc tgcgcagtct tccctgacgg agagcatgaa gcggctgaaa caggtaatgc
    11821 tgcctttgcc ctgtggcagc cctacagttg ctgcaagaaa cggggacaga cattcctggg
    11881 cagtacgaat ttttaagtaa ggggaagtga tgaaacagaa aatgacgata cctgttctgg
    11941 ctgtggtgct ttgtgcgggt acggtacagg ctgacggaaa ctttttgtcc ctgccgaaag
    12001 tgaatgacag tgcgtttggc tacggtaaat ccgtggacgg cgcagtatca gacaaactgt
    12061 tttataccct gggagggggc tcggtgatat cgcagccggc aacgcgcagc aatatgcaga
    12121 aacttggggt ggatctcggg tggagcagtg atctgatgtg tggcaatttt gacctgaaaa
    12181 caacagtggg taaccagctg aacgggatca ctgatggttt caaaaacctg atgggggatg
    12241 tgattcaggg ggcgagcgga gccgtggcga gtctgccggc aatggtgata cagcgggcta
    12301 atccggggct gtatgacatg ctgaccaacg gtgtactgca ggcgaatgtg gcattcgaca
    12361 aagcgcagct caattgtcag aacatggcga aacgcatgat ggagttcacg gacagcaata
    12421 agtggacgca ggcggccatg ctcgaggagt acaaaaaaat tgtgaatacg ggggacggtg
    12481 atgcgatcaa aggggatgaa gcaggtcgtc aggttaccgg ggtggagggg caacgctgga
    12541 tcggagggca gcagcgtggt ggagcgggac agtctgctat ccggccgaca catgatctga
    12601 cagctgccgg tttcaatatg atgaacagtc tgccttcgac cagtacatcc agtgtcagtc
    12661 ccgggaactg taacgggagt gcctgcatga aataccgtaa tgcagaggaa gctgcccggg
    12721 cggtggtgaa ggttctgggg gataaatcca tgcggacctg tggtgatgca aaggcatgta
    12781 ccagcgggga tgctgatcag cagccgggga ccagtattgc cggcacgggg tttgcgccga
    12841 tgcttgaaga agcgacgcgg gttaacacgg aactgctggc aggtatggtc aatggtcgtg
    12901 acaagccgac agccgggaat ctggcaaaac tgaaagccgg gagtctgtct gtgacagcgg
    12961 gagtgatccg tgccctgcag cgtgatccgg acaatggtgc cctgacgtcg cgtctggcgg
    13021 gagaactggc catggcggat accgttgaaa cggcgctgct gatgcgcagg atgctgctca
    13081 cggggatggc cgagccgtat gctgcagctc agcctgctgc actggaagag ggggaacgcc
    13141 gtattgaggc gctggatcgg gaaattatgg cgctgaaaaa cgagatggag ctgaagcggg
    13201 atctggcgcg taacagtgtt ctgaccatca ttgaacggga taacgagcgt gtggggaata
    13261 acccgatgat ccagcaggct gacaatgctg acagccgtat ccattctctc gatgccccgg
    13321 ataaggagtg agaggatgag cagaatttat ggccgggtga aaaagaggat gaaggtattc
    13381 gccctgtata cgctgttgct gacagggatt accgtgtttt ccctctggct ggccgatacc
    13441 ggtatacgtc acgcagaagg atttgcggcc ctgcgggagt ggatgtatga gacacgttat
    13501 ggctggctgg gatggcgtct gtgtctttac agtgcgatcg gatggctggg gtggaggatc
    13561 tggcatgcgc cggggttcag ggcagaatac cgggcttctt ttctgcgtat ggccacagtc
    13621 agtgttctgt ttatcctgct gtgtgaatac gccatttttg ctgagccggg gcgttgagca
    13681 atcatgacca caaataacta tctggaatat tttctgactc tgctgggctg ggtggtgaat
    13741 aacggtctgt ggaatgtgtt gatcagcaca ggactgtttg ccctgccact ggcattcagg
    13801 atcatcggga tctggctgaa agtcagggaa gaaggtgcgg atgagggaaa caaagggatg
    13861 ctttccctgc cgcgtattga gcatgttctc tatggtgctt tttttgtgat gatatcctgc
    13921 tgtgttcctc tggtacaggt gacgctggat acgctgtcat tcgataaatc cagggcacag
    13981 acctgtgggg tatggacacc tgaagagccc ggagagacgg ggtacagtgg ggtgatcagc
    14041 agtatagggg atcagactgc tgctgccccg gtatggtggg tggtggttca taaattgtcg
    14101 aaaggtgtca cccaggcggc agtggcttcg attccgtgcc ggcctgattt gcgccagttg
    14161 cgttttgaga tccagcgaac gtttatagaa aacagggcac ttgctgatga gctgcaggac
    14221 tttacgaacg actgttattc actggcactg tatatgtgga aacagcgtga tcaggggcag
    14281 agtaaagata aaaaagtgct tgatgatatc tcctggatag ggagcgccac attcatgcag
    14341 ggggattacg ggacgctgca gtcaaagatg ccgcgggctg cgttcccgtg gaatacagca
    14401 cgtgacagcg gacgaccgga tacggggcgg ggaggctatc cgacatgccg ggagtggtgg
    14461 agtgacagta gtgtggggct gaaaaaacgg gtcatggagg aggtcagtga tcattactgg
    14521 ttacgtctgt cagctgcaat gaagatgatt ggcagtagtc aggtggatta taaggaggag
    14581 gtgatcaagc gtctggtgag tccggcaaac ctgacgctct ctcagggggg agaggtgtat
    14641 cgtggctatg gtggcaatgc agatatgacg accatgaatg cggtggcccg gattgcggga
    14701 acggcgggca gtgcggtagc agcactgggt gcgtttccgg cactggatgc aatgcggcag
    14761 gcactgccga tggtacagtc gataatactg atggcgattt acatcatgat accgctgatt
    14821 ctggcgtttg cggcatatga actgtcgacg ttaatcacac tgacgtttgt ggtgtttgcg
    14881 gtacactttc tgacattctg gtgggagctt gcacggtggc ttgacagctg gatgctgacg
    14941 gcgctgtaca gttcggatac acatacccgg ttcaatatga tgggattcca gaacacatcg
    15001 gatgatctga taatgaatct ggtgatgggg acgatgtttc tcgtcttgcc ggcagtgtgg
    15061 ctgggagcgt tgtcctgggc tggggtaagt gtcggaacgg ttatggggga tgcaattaac
    15121 aaaggaacaa gtaatgtatc taaggcagca gcaaaaagtg gagatttagc taataagtaa
    15181 aattcttagg gtttccttag ctgggataat tggggaaacc cttgcttttc ttagaaatca
    15241 gcattgtcat ggtgatgttt ccacgggtca tataatgcaa agggtctcat tggtactgaa
    15301 taatcagtat ctttttcatt acttagtgaa aacatatttt ggggtttatc ttgagcaggt
    15361 gtgaaatctg gtaaaacggc acagactgct tttttcagca gggaagtgaa ggtccatgga
    15421 ttatccacag gcttacaacg ccacattgct ttttcagatt cgttattcat atctataccc
    15481 tcagcagcag gataaataac acagtgttat catacgtctt gtatgataaa tgtgcaatca
    15541 catgtcttcc atttgatgag aggctgcgat gccacattac ccttgggctc ggggattctg
    15601 ttgaaatgat ctagctgaga atgtatctta ttgttatgtt ataacatgag tgaagagaga
    15661 tgaagccaaa tatccatcct gaataccgca cagtagtgtt ccatgacacg agtgtggatg
    15721 agtactttaa aataggttct accatcaaaa cagatcgcga gattgaactg gatggtgtga
    15781 cgtatccgta tgtgacgatt gacgtttcct ccaaatcgca tccgttctat accggtaaac
    15841 tcagaacggt ggcatcagaa ggcaatgttg cccgctttac tcagcgtttt ggtcgtttcg
    15901 ttgggactca gaagggagaa tgatgagagt gagagtctta acaggctcca tgcgtggtgg
    15961 atttgctcct atatttccag acatctggta tcacttaacc cattactgcc cgccgccgca
    16021 gatattcccg tggagagcga taacccagcg cactatgtgg atgccattcg ttataatgct
    16081 cgaacgcttc tgcaaggttc tttgctgccg ttaacccgtc tggtttgggc atgatactga
    16141 tgtagattga agaagggatt gtgagttttt ccttcggctt ttaacgtaac ttcctgtttt
    16201 cccgatattc cacagcaaga ttaattttta gcggagctga atgaataaaa tcttcatctg
    16261 gtgtttttgt tgcgcgtaaa tgcaggtgta actgtggatg ttatccactg aagagctgct
    16321 gatgaggatg aagacatgct gactaaaaaa atgttactcg tttgtgcttc gtgtgttgcg
    16381 ctgagtgcct gttctgcaca ggaaattgca gagctgaaca ggcagataag tgacggtgca
    16441 tttgctgtga caaaagtact gaacgtaggt gtaaaccagg cagatgatga tctcaaatat
    16501 ctgtctcagg caccccgggc aaaaagagag aaacgtgatc atgtcataga agtccctgtt
    16561 gatgtggata cagctgcggg aagactgaaa cggtactata agttttattc aaatgaagaa
    16621 gtcgatgcga ttcgtaataa cggacatgcg aacgggaagt gggccgcagc agcaataagt
    16681 cagaacgggc atatgtggga tgccatgccc ggtagttatt ataaaatggg cagtgactgg
    16741 gatggtggcg agtttgatga tcatctgacg cttgaacttg aaaaaaatgg caaaggaagt
    16801 cggatatata taacgtatgc atctccggca ccggggcatc tgaaagataa atttattgag
    16861 ccattagtta aacgtgtgaa ggatgtggct gaaggccggg tcagataaaa ttaacccgct
    16921 ggcattgtcg ttgtgatggg ctcctctgga ggggcgatta tgctcagagg ggctgaaagt
    16981 aagggacgca gagggcgacg accagcgacg tctggaagag ggcgtgatat gtaaggaagc
    17041 acaccctgac ggcatatcat aaaaaaacca gatatgcgcc agttaaaagt ataaataaag
    17101 agctgataaa attaacatat tttgaaaatg atgaatttat acgccctctt atagttaaac
    17161 atatacatat atatattaag tcagtgagta atgacacaat tataaaaatg atacaaagta
    17221 acaaaaagtc agtattaaag ttactgttgt tttttatgaa tgatggcata aatgcagcaa
    17281 agaacaaaaa ggcctttgga ttgagcatcg gtattaaaaa agggatattt gctttgtttc
    17341 ctttagatcc ttttctcttg atgagacgag taataccaag aaaaatcaga tatccaccac
    17401 cacaagtttt tattgtgtca agatgttttt ctaaattgat tgccagtgct atagaaaata
    17461 gtgtgactag catgacaaca atttcgccaa atcttatata tatacttgtc gttattgctt
    17521 tttttgcacc atatgtcaga ctttcagata tgattaatgc cacccccgga ccaatcgata
    17581 ctgtttgtga taaggttata atagagaatg ttattaatgt tgagaagtcc atgggattat
    17641 ataactcctg ttattctggt tggaacctta aaataaacat actccatagg tatggctgcc
    17701 tcaggaagca acattataaa
//
LOCUS       DAZGQL010000098        17705 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.098, whole genome shotgun sequence.
ACCESSION   DAZGQL010000098 DAZGQL010000000
VERSION     DAZGQL010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17705
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.098"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(46..699)
                     /gene="fdnI"
                     /locus_tag="ACHKIR_003980"
     CDS             complement(46..699)
                     /gene="fdnI"
                     /locus_tag="ACHKIR_003980"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HFO9991656.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(692..1576)
                     /gene="fdnH"
                     /locus_tag="ACHKIR_003981"
     CDS             complement(692..1576)
                     /gene="fdnH"
                     /locus_tag="ACHKIR_003981"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HFO9991657.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVNLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEEHHE"
     gene            complement(1589..4636)
                     /gene="fdnG"
                     /locus_tag="ACHKIR_003982"
     CDS             complement(1589..4636)
                     /gene="fdnG"
                     /locus_tag="ACHKIR_003982"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(4049..4051),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFO9991658.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGIANGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            4868..5749
                     /gene="yddG"
                     /locus_tag="ACHKIR_003983"
     CDS             4868..5749
                     /gene="yddG"
                     /locus_tag="ACHKIR_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415990.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HFO9991659.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGDNGLHYDEIINNITTSPLSYF
                     LSFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGASLWVYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            6022..7410
                     /gene="narU"
                     /locus_tag="ACHKIR_003984"
     CDS             6022..7410
                     /gene="narU"
                     /locus_tag="ACHKIR_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HFO9991660.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     TVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QREAITETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            7505..11245
                     /gene="narZ"
                     /locus_tag="ACHKIR_003985"
     CDS             7505..11245
                     /gene="narZ"
                     /locus_tag="ACHKIR_003985"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HFO9991661.1"
                     /translation="MSKLLDRFRYFKQKGETFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKTYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMVRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGTETELLLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLADGNTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVF
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGPTEGIQPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDMVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLAQHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            11242..12786
                     /gene="narH"
                     /locus_tag="ACHKIR_003986"
     CDS             11242..12786
                     /gene="narH"
                     /locus_tag="ACHKIR_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFO9991662.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIEAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLNVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            12786..13481
                     /gene="narW"
                     /locus_tag="ACHKIR_003987"
     CDS             12786..13481
                     /gene="narW"
                     /locus_tag="ACHKIR_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFO9991663.1"
                     /translation="MQILKVIGLLMEYPDELLWECKEDALALIRRDAPMLTDFALDLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLNCRE
                     LPDYLPLYLEYLSILPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGS
                     PLSSDSVTKQIRSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            13478..14158
                     /gene="narI"
                     /locus_tag="ACHKIR_003988"
     CDS             13478..14158
                     /gene="narI"
                     /locus_tag="ACHKIR_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFO9991664.1"
                     /translation="MIQYLNVFFYDIYPYLCGTVFILGSWLRYDYGQYTWRAASSQML
                     DKRGMVLWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAVKQQMAMILGGICGVLT
                     LIGGAGLLVRRLTNPRIRATSSGADILILAILLIQCILGLTTIPFSAQHPDGSEMMKL
                     VGWAQSIVTFQGGASTHLDGVAFIFRVHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            14238..15131
                     /locus_tag="ACHKIR_003989"
     CDS             14238..15131
                     /locus_tag="ACHKIR_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HFO9991665.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTVWQTSLAGK
                     HRVTIEKHNDDYRISLEQGTPGFESPLEGETRAAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLNALTAISKQIGCNGFFPFQIRPDKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGNVLRVKGHQGRALGRDGVIEVTVTIRDNQP
                     EKVTISGVAVILFHAEWAIEL"
     gene            complement(15227..16072)
                     /gene="nhoA"
                     /locus_tag="ACHKIR_003990"
     CDS             complement(15227..16072)
                     /gene="nhoA"
                     /locus_tag="ACHKIR_003990"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HFO9991666.1"
                     /translation="MTPILNHYFARINWSGAAAANIDTLRALHLKHNCTIPFENLDVL
                     LPREIQLDDQSLEEKLVIARRGGYCFEQNGVFERVLRELGFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVELEGEKWIADVGFGGQTLTAPIRLMPDIMQTTPHGEYRLLQEGDDWVL
                     QFNHHQHWQSMYRFDLCEQQQSDYVMGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHAVEQRNLPDVASLYAVMQEQFGLGVDDAKHGFTVDELALVMAAFDTH
                     PEAGK"
     gene            16247..16816
                     /locus_tag="ACHKIR_003991"
     CDS             16247..16816
                     /locus_tag="ACHKIR_003991"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFO9991667.1"
                     /translation="MSQFIPVELHHASRLLNHGPTVLITSFDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIERNGKFGIVIPGVAATNWTWAVGSVSGREEDKFYCY
                     GIPVVKGPVLGLPLVEEKCLAWMECRLLPATSAQEKYDTLFGEVVSAAADARVFVEGR
                     WQFDDDKLNTLHHLGAGTFVTSGKRVTAG"
     gene            complement(16970..17350)
                     /locus_tag="ACHKIR_003992"
     CDS             complement(16970..17350)
                     /locus_tag="ACHKIR_003992"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO9991668.1"
                     /translation="MIIRPVKPSDVEPLLDMLLASGQFNQDGLHHVQKTLTHYFSGQS
                     ADLWFSAEHLGLAGIAYCAPEVMTNDVWNLLMHWISPSHQRQGVGHLLINEIEKELTI
                     KQARLLLVETSSYVDFSAARAFYT"
BASE COUNT         4180 a         4655 c         4507 g         4363 t
ORIGIN      
        1 tggcgcttct taaagcgcca ttttcagctt aaagttcaaa agagattata tcccttcttc
       61 actctctttt ttcgcttccg ccttctcgat ttcacgatac cagcgcggat ggtgtttctt
      121 cgcccagcga cggctcacct tcccttcgat catcccttta atcgatcctt tcacccagaa
      181 tgccatatac atatggatca ggatggcgtg gatcaggatg atacccgcag ccgcgtgaat
      241 cagcaggctg tagcgaacaa cctgcatcgg gaagtactgc gcaaagtacg gacgccagat
      301 aatcaccccg gtcaccagca gcacgaaaat catgctcatg atcgaccaga acatcatctt
      361 ctgcccggcg ttgtacttac caacatccgc cactttgtgc tcattgcctt ttaatacttc
      421 gacaatgttc aacagccacg gaatatcctt cttatccggg atattgtgat gtacaaaacg
      481 cacaaacata aacatcagcg cgacgaaaat cgcaatgccg aagaacgggt gcaaaatgcg
      541 tcccatctgc ggcgtaccga aggtttgcgt cagccattgc agcgtcggga agaagaacga
      601 aatcccggac agcgccacca ggaagaagca aatcaccacg gtccagtgac aggcgcgatc
      661 aataaacttg gtgcgcacaa tcattttcga cttactcatg atgctcctcc tcgtcatcgt
      721 ccacttcctt attcgggcca ataccgatgt agtggaaaat caacccggca aaagtggcga
      781 taaagccagc cgctgccagc ggtttcaacg cgcctttcca caagttcacc gatgtgtcga
      841 tcttcggatc tttcggcaga ccgtgataca gctccggctg atcggcgtga tgcaacacgt
      901 acatgacgtg cgtaccaccg accccttccg ggttgtagac gccagcatgt tcgtaaccac
      961 gcgctttcag tttcgctacg cgctgttccg ccagctccag catctccttc ttggtgccga
     1021 agtggatagc cccggtcgga caggttttca cacaagccgg ttcctggccg acgctgacgc
     1081 gatcgacgca gagcgtgcat ttatataccc ggttatcctc tttgttgagg cgcggaatat
     1141 taaacggaca cccggcaatg cagtaaccac agccgatgca gttttccgac tggaaatcaa
     1201 caatcccgtt agcgtactga atgattgcac cagcagacgg gcacgccttc aggcagcccg
     1261 gatcttcaca gtgcatacag ccatctttac ggatcagcca ctccagcttg ccgttctgtt
     1321 cggtttcgct aaagcgcatc accgtccagg acttggcgct cagatcggcg gggttatcgt
     1381 aaaccccgac gcagtgcccc acttcatcac ggatgtcgtt ccactccgaa cacgccacct
     1441 gacaggcttt acagccgata caggtggaaa cgtcgataag tttcgcgact tctgctttgt
     1501 aatcacgcac ctgagaaggc ggcgtgatgg agttagttgc ggaccttttg ataatgtcct
     1561 gcgtttccat agccatctgt tcgccccctt acgccttctc gatgtttact aagaacgctt
     1621 tatattccgg cgtttgcgag tttgcatcac cgacattcgg cgtcagagtg ttagcgatat
     1681 aacctttacg cgcgacaccc tcaaagcccc agtggattgg aatacccacc gtttcaacct
     1741 gctgaccgtt tacattcagc ggtttcagac ggcgcgttac cacagccacc gcgcggataa
     1801 agccacgctt gctggagaca gtgacacgat cgccattggc aatgcctttc gccgccgcca
     1861 gcgtttcgct gatttccaca aactgttccg gctgagcaat tgcgttgagc aatgcgtgct
     1921 tggtccaggt gtggaagtgc tccgtcagac gataggtcgt acccacatac gggaactgct
     1981 cttttttacc catccgcagt gcgtcttgtt catacagacg aacaaccggg ttagacacca
     2041 cgttcggatg caacgggtta gtgcccagcg gcgtttcaat tggctcatag tgttccggga
     2101 acggaccttc cgccattttg ttgatggcaa acaggcgtcc catcccttcc ggctgcatga
     2161 taaacggccc ggttggcgta cccggtgcgg cattgccgaa gtccggaata tcgttacccg
     2221 tccacttgct accgttccac tggatcagca tccgtttcgg atcccacggt ttaccgttga
     2281 tatccgccga agcacggttg tagagtacgc gacggttgag cggccacgcc caggcccatc
     2341 ccagcgtatt ccccagaccg gacgggtcgg agttatcgcg gttagccatc tggttgccct
     2401 gctcggtcca gctaccggtg tagatccagc aagaagatgc ggttgtaccg tcatcacgca
     2461 gatgcgcaaa gctactcagc aactgacctt tcttcgcaat cagcacgcca ttagcgtcat
     2521 agagatcttc cagcgcatag ccgttgttct ctttcgccac ttcgtcagac tgcggttcgt
     2581 gcggctgctt gtagttccag ctcatcttca tcagcggttc tacgccttta ccaccctcgg
     2641 cctggtacag ctcgcgcaga tgatggtaga tacccgccag aatttcgccg tcgttacgtg
     2701 cttcgcccgg cgcgtcctga cctttccagt gccactgcaa ccagcgaccg gagttagcga
     2761 tagaaccatc ttcttcagca aagcaggtcg aaggcagacg gaatacttca gtctgaatag
     2821 atgccggatc gacatcgttc gactcgccgt ggttctgcca gaaggtagag gtttcagtca
     2881 ccagcggatc gataaccacc atgtacttca gtttgctcag gcagctcacc actttgtttt
     2941 tgtccgggaa ggacgcaacc gggttaaagc cctggcagaa ataaccggtg actttacctt
     3001 catccatcat gttgaaatac ttgatgacgt cgtaggtctg gtcccacttc ggcagccagt
     3061 cgtagcccca gttgttctct ttctgcgcgg catcgccgta gaaagatttc atcaggctaa
     3121 cgaaaaactt cggatagttg ctccagtagt tcacctgatc agccagcgtc gctttcggcg
     3181 tgttcgcttc cagatacgac tgcaaatcaa cctgtttttc tgacggcagc gtcagataac
     3241 ctggcaggct ggtagagagc aggcctaagt cagtcaggcc ctgaatgttg gagtgaccac
     3301 gcaatgcgtt cacgccgcca ccggccatac ccatgttacc gagcagcaac tggatcatcg
     3361 ccatcgtacg gatgttctgc gcacccacgg tgtgctgcgt ccagcccagc gcgtacagga
     3421 aggtggttgt gcgatccggt gcgctggtgg aggccagcac ttcacacact ttcaggaagt
     3481 cggcttttgg cgtaccacag atgttttcaa caacgtccgg cgtgtagcgg gaaacgtgcg
     3541 ctttcagcag gttccacaca cagcgcggat gagtcagtgt ttcatcgcgt ttcgcatagc
     3601 cgttttcatc gaactgatag ttccaggacg atttatcgta ttgccgtttt tcagcgtcgt
     3661 agccgctgaa cagaccgtct tcgaaagcaa aatcatcacg caccagcagg ctggcattgg
     3721 tgtaatgctt aacgtattcg gcattgattt tgttgttttc gatcaggtag cgcaaaacgc
     3781 cagacaggaa cgtaatgtcc gtaccggaac gaataggtgc gtaaatatcc gctacagaag
     3841 cggtacgcgt aaaacgggga tcgacaacga tcaaggttgc atcgttgttg tttttcgctt
     3901 ccatcgccca gcggaaaccg acgggatgcg cttcagcagc gttaccgccc atcaccatta
     3961 cgacgttagc gtttttgata tccacccagt ggttggtcat cgcaccgcga ccaaatgttg
     4021 gagcaagact tgctaccgtt ggtccgtgtc agacgcgcgc ctggttgtct actgccagca
     4081 tcccgaggga gcgggcaaat ttttgagtca gcatcccggt ttcgttgctg gcaccggagg
     4141 cacacagcat accggtagaa agccaacggt ttaccgttac gccctgctcg ttcttttcaa
     4201 taaagttagc gtcacggtca gctttcatca gcttcgcaat acgggagaat gcttcttccc
     4261 agctaatgcg ctgccatttg tcagaacctg gcgcacgata ttccgggtag cgcagacggt
     4321 tttcgctgtt gacgtaatcc agcaaaccgg cccctttcgg gcacagcgca ccacggctta
     4381 ccggatgatc cgggtcacct tcaatgtgat aaatcgcttc tctggcgttt ttcgcgccat
     4441 cacccaggct atacatcaat agcccgcaac ctacggaaca gtatgtgcag gtgttacgga
     4501 tctctttcgc gcgtaataat ttgtagtttc gcgcctgagc cagtgcttgc ttcggggcaa
     4561 agcccaatgc cgctaccgtt gttccagcca taccgcccgc gcagatttta aaaaattgtc
     4621 tgcgactgac gtccattgct ttcctctttt ttcaggggta ttactgcgcg aggaaactaa
     4681 cagtaaaatt ctgaatcttt ttgcgcgaaa tcaaaaagaa cacgtgtttg ccacaaaata
     4741 atccgcctgc gcaaattgac ctacctcaat agcggtagaa aaacgcacca ctgcctgaca
     4801 ggccagttaa aaaaatgcta taaaattcag cttaattttt aacggcaaga gagacaaaac
     4861 agcgagcatg acacgacaaa aagcaacgct catagggcta atagcgatcg tcctgtggag
     4921 cacgatggta ggattgattc gcggtgtcag tgaggggctc ggcccggtcg gcggcgcagc
     4981 tgctatctat tcattaagcg ggctgctgtt aatcttcacg gttggatttc cgcgtattcg
     5041 gcagatcccg aaaggctatt tactcgccgg gagtctgtta ttcgtcagct atgaaatctg
     5101 tctggcgctt tccttagggt atgcggcgac ccgtcatcag gcgattgaag tggggatggt
     5161 gaactatcta tggcccagcc tgacaattct ctttgccatt ctgtttaatg gtcagaaaac
     5221 caactggctg attgtaccag gattattatt agccctggtc ggcgtctgct gggtgttagg
     5281 tggtgacaat gggttacatt atgatgaaat catcaataat atcaccacca gcccattgag
     5341 ttatttcctg tcgtttattg gtgcgtttat ctgggcagcc tactgcacag taacgaataa
     5401 atacgcacgc ggatttaacg gaattaccgt ttttgtcctg ctaacgggag caagtctgtg
     5461 ggtttactat tttctcacgc cacaaccaga aatggtattt agcacgccag tcatgattaa
     5521 actcatctct gcggcattta ccttaggatt tgcttatgct gcatggaacg tcggtatatt
     5581 acatggcaac gtcaccatta tggcggtagg ttcgtatttt acgcccgtcc tttcctcagc
     5641 gcttgcagcc gtgctgctca gcgccccgct gtcgttctcg ttctggcaag gcgcgctaat
     5701 ggtctgcggc ggttccctgc tctgctggct ggcgacacgt cgtggctaaa ataattgtcg
     5761 ggtctacggc ccggcagtta atcagataaa ttctgacaat attaaaaaat aaatttcaaa
     5821 taccagaaag ccattttagt tacatttagt aacacatatc tgtctgttta atccacattc
     5881 tgcccccgca gttacgcttt cttatctatt cttctaatca gcacttcacc tgattcatcc
     5941 ttaattctaa taactggcta ttgcgcctta cctcctgttt tgtctttttt tcattaccaa
     6001 tgtgtgcatg tgaggaacaa tatggcactg caaaatgaga aaaatagtcg ttatctttta
     6061 cgcgactgga aaccagaaaa tccggccttc tgggaaaata aaggaaagca tattgcacga
     6121 agaaacctct ggatatcagt cagttgccta cttcttgcct tctgtgtctg gatgctattt
     6181 agcgcagtta ctgttaatct caataaaatc ggttttaatt tcactaccga tcaactcttt
     6241 ttattaaccg cattaccctc cgtttctggc gcattattgc gtgtccccta ctcctttatg
     6301 gtgcctatat tcggtggacg ccgatggacg gtttttagta ctgcaatcct gattattcct
     6361 tgcgtctggc tcggaattgc cgtgcagaat ccgaatactc cttttgggat atttatcgtt
     6421 atcgctttgc tatgcggttt tgcaggtgcg aactttgctt cgagcatggg caatatcagt
     6481 ttcttctttc caaaagccaa acaagggagc gctcttggga ttaatggcgg attaggaaac
     6541 ttaggtgtaa gtgtgatgca gctggttgca ccgctggtca tttttgtccc cgtatttgcc
     6601 tttctcggcg tcaatggcgt accgcaggcc gacggttcgg tgatgtcgct ggcgaatgcc
     6661 gcatggattt gggtgccatt actggcgatt gccacgatcg ccgcctggtc agggatgaat
     6721 gatatcgcca gttcacgcgc ctcaattgcc gaccagctcc ctgtcttaca acgcctgcat
     6781 ctctggctgc tgagcctgct ttaccttgcc accttcggtt cgtttatcgg tttttctgcg
     6841 ggttttgcca tgctggcaaa aacccagttc ccggatgtga atattctgcg cctggcgttc
     6901 tttggcccat ttatcggtgc catcgcgcga tcggttggtg gtgctatttc cgataaattc
     6961 ggcggcgtgc gggtgacgtt gatcaacttc atttttatgg cgattttcag cgccctgctg
     7021 ttccttacct taccgggcac aggctccggt aatttcatcg cattttacgc cgtatttatg
     7081 gggctgtttc tgaccgcggg tctgggaagt ggttctactt tccagatgat cgccgtcatc
     7141 tttcgccaga taacaattta tcgggtgaag atgaaaggcg gtagtgatga gcaagctcaa
     7201 agagaagcca tcaccgaaac ggcagccgct ctgggcttta tctcagccat tggcgcagtg
     7261 ggcggctttt ttattccgca ggcgtttggc atgtcgctca atatgaccgg ctctccggtg
     7321 ggcgcgatga aagtgttttt aatcttctac atcgtttgtg tgctactgac ctggctggtt
     7381 tatggtcggc ggaagttcag ccaaaaataa gagatcgatg cctgacacgt cttatcaggc
     7441 tacaaccgtg tcgcatccga caaggcatca gatcccaatt cattcctgga gcaggagtta
     7501 tgtcatgagt aaacttttgg atcgctttcg ctacttcaaa caaaagggcg aaacctttgc
     7561 cgatggtcac ggacaggtga tgcatagcaa ccgcgactgg gaggacagct atcgccagcg
     7621 ttggcagttc gacaaaatcg tgcgttccac ccacggtgtt aactgtacag gctcctgtag
     7681 ctggaaaatc tacgttaaaa atggtctggt gacctgggaa atccaacaga ccgactaccc
     7741 gcgcactcgc cctgacctgc caaatcatga acctcgcggc tgcccgcgtg gcgcaagtta
     7801 ctcctggtat ctctacagcg ctaaccgcct gaaataccct ctcattcgta aacgactgat
     7861 tgaactgtgg cgcgaagccc tcaagcaaca cagcgatccg gtactggcgt gggcatcgat
     7921 tatgaacgat ccgcaaaagt gcctgagcta caaacaagta cgtggacgcg gcggctttat
     7981 ccgctctaac tggcaggagc taaatcagct gattgctgcc gctaacgtct ggaccatcaa
     8041 aacctacggc ccggatcgcg ttgccggttt ctcgccgatc ccggcgatgt cgatggtttc
     8101 ttacgccgcc ggaacacgtt atctttcact attaggtggc acctgtttaa gcttctacga
     8161 ctggtattgc gacctgccgc ccgcctcgcc gatgacctgg ggcgagcaaa ccgacgtacc
     8221 ggaatctgcc gactggtata actccagtta catcatcgcc tgggggtcta acgtaccgca
     8281 gacacgtacg ccggacgccc atttctttac cgaagtacgc tacaaaggca ctaaaaccat
     8341 cgccattacc cctgactact ctgaagtggc caaattgtgc gaccagtggc tggcaccgaa
     8401 acaaggcact gatagtgccc tggcaatggc aatgggccat gtcattttaa aagagtttca
     8461 tctcgataat cccagcgatt attttattaa ctactgccgc cgctacagcg acatgccgat
     8521 gctggtaatg ctggaacctc gcgacgacgg cagctacgtt cccgggcgca tggtccgcgc
     8581 atctgacctg gtggatggac tgggcgaaag caataatccg cagtggaaaa ccgtggcagt
     8641 taataccgca ggtgaactgg tagtgccgaa cggttcgatt ggttttcgct ggggagaaaa
     8701 aggcaaatgg aatctggaat ccattgccgc cggtacggaa accgaattgt tgttaactct
     8761 gctcggtcaa catgatgccg ttgcaggcgt ggccttcccc tactttggcg gcattgaaaa
     8821 tccgcatttt cgcagcgtaa aacacaatcc ggtcctggtg cgccaattgc ccgttaaaaa
     8881 cctgacatta gccgatggca acacctgtcc ggtcgtcagc gtttatgatt tggtactggc
     8941 gaattatggc ctcgatcgcg ggctggaaga tgaaaacagc gcgaaagatt acgctgaaat
     9001 caaaccgtac accccagcct ggggtgagca aattaccggc gtgccgcgcc agtatattga
     9061 aaccatcgcc cgtgaatttg ccgataccgc ccataaaacg catgggcgct cgatgattat
     9121 cctcggtgct ggcgtcaacc attggtatca catggacatg aactaccgtg ggatgatcaa
     9181 catgctgatc ttctgcggtt gtgtcgggca aagcggtggc ggctgggcac actatgtcgg
     9241 tcaggaaaaa ctgcgcccgc aaaccggctg gttgccgctg gcctttgcgc tcgactggaa
     9301 ccgaccaccg cgccagatga acagcacctc gtttttctac aatcattcca gccaatggcg
     9361 ctatgaaaaa gtctttgctc aggagttact ttcaccgctc gccgatgcca gtaagtacag
     9421 cggtcatctg attgatttca acgttcgtgc cgaacgtatg ggctggctac cttctgcgcc
     9481 gcagctgggg cgtaacccgc tcgggattaa agctgaagcc gacaaggccg gattatcccc
     9541 cacagaattt accgcccagg cgctgaaatc gggcgattta cgcatggcct gcgaacaacc
     9601 agatagcggc agcaatcatc cgcgtaattt gtttgtctgg cgttctaacc tgcttggctc
     9661 ctccggcaaa ggccacgagt atatgcagaa gtatctgctg ggtacggaaa gtggcattca
     9721 gggggaagaa cttggaccga cggagggaat ccaaccggaa gaagttgagt ggcaaactgc
     9781 agcgattgaa ggcaagctcg acctgctggt gacgcttgac ttccgcatgt ccagcacctg
     9841 cctgttctcc gatatcgttc tgcccaccgc cacctggtac gaaaaagacg atatgaatac
     9901 ctcggatatg catccgttta ttcatccgct ttctgcggcg gtcgatccgg cgtgggaatc
     9961 acgcagcgac tgggaaatct acaaaggtat cgctaaagcc ttttcgcaag tgtgcgtggg
    10021 gcatctcggc aaagaaaccg acatggtatt acaaccactg ctgcatgact ccccggcaga
    10081 gctttcacag ccgtgtgaag tgctcgactg gcgcaaaggt gaatgcgatc tgattccagg
    10141 caaaaccgcg ccgaatattg tggcggtgga gcgcgactac cctgctacct atgaacgctt
    10201 tacctcactc gggccattga tggacaaact tggcaacggc ggtaaaggga tttcgtggaa
    10261 tacccaggat gaaatcgatt tcctcggtaa actcaattac accaagcgtg atggcccggc
    10321 acaggggcgt ccgctgattg ataccgcgat tgacgcatca gaagtgattc tggcgctggc
    10381 accggaaacc aacggccatg ttgcagtcaa agcgtggcaa gcgctgggcg agatcaccgg
    10441 acgcgaacat acccatctgg cgcagcacaa agaggacgag aagattcgct ttcgcgatat
    10501 tcaggcgcag ccgcgtaaaa ttatctccag ccccacatgg tctggtctgg aaagcgatca
    10561 cgtttcctat aacgcgggat acaccaacgt tcatgagtta attccgtggc gcacgctgtc
    10621 gggacgccag caactctatc aggatcatcc gtggatgcgt gcttttggtg aaagtctggt
    10681 ggcttatcgc ccgcctatcg acacccgcag tgtcagtgaa atgcgccaga taccgccaaa
    10741 cggcttcccg gagaaagccc ttaacttcct gacaccgcac cagaaatggg gcattcactc
    10801 aacctacagt gaaaacctgc taatgctgac gctctctcgc ggtggaccga ttgtctggat
    10861 cagcgaaacc gatgcccgtg aactgaccat tgtcgataac gactgggtgg aagtgtttaa
    10921 cgccaatggc gcgctgacgg cccgcgcggt ggtcagccag cgtgtaccac cgggcatgac
    10981 catgatgtat cacgctcagg aacgcattat gaatattcca ggttcggaag taaccggcat
    11041 gcgcggcggc attcataact cggtcacccg cgtttgtccg aaaccaacgc atatgattgg
    11101 cggttacgcg cagctggcct ggggctttaa ttactacggc accgtcggct cgaaccgtga
    11161 cgaattcatc atgatccgca agatgaagaa cgttaactgg ctggatgatg aaggtcgcga
    11221 tcaggtacag gaggcgaaaa aatgaaaata cgttcgcaag tcggcatggt gcttaacctc
    11281 gataaatgta tcggctgcca tacctgttcg gtgacctgta aaaacgtctg gaccggacgc
    11341 gaaggcatgg agtacgcatg gtttaacaac gtcgaaacca aaccgggcat tggttatccg
    11401 aaaaactggg aagatcagga agaatggcaa ggcggctggg tgcgtgatgt gaatggcaag
    11461 atccgcccgc gtctgggcag caagatgggc gtaataacca aaatcttcgc caacccggtg
    11521 gtgccgcaga ttgatgatta ctacgaacct ttcaccttcg actacgaaca tttgcatagc
    11581 gcaccggaag gcaaacatat ccctactgct cgcccgcgtt cactgattga cggcaaacgg
    11641 atggacaaag tgatctgggg gccaaactgg gaagaactgc tgggcggcga atttgaaaaa
    11701 cgcgctcgcg accgcaactt cgaggccatg caaaaggaga tgtacgggca gtttgaaaac
    11761 accttcatga tgtacctgcc gcgcctgtgc gaacactgcc tcaatcccag ctgcgtggcg
    11821 acctgcccaa gcggtgctat ctacaaacgt gaagaagacg gcattgtgct gattgatcag
    11881 gataaatgcc gtggctggcg tttgtgcatt agcggttgtc cgtacaaaaa aatctacttc
    11941 aactggaaaa gcggcaagtc agaaaaatgt atcttctgtt acccacgaat tgagtcaggc
    12001 caacccaccg tgtgctcaga aacctgcgtg ggtcgcatcc ggtatctggg cgtgctgctt
    12061 tacgacgccg accgcatcga ggaagcggcg agcaccgagc gcgaagttga cctctatgaa
    12121 cgtcagtgcg aagtgttcct cgatccgcac gatccctcag tgatcgaaga agccctgaaa
    12181 caaggtattc cacaaaacgt gattgaagct gcccagcgtt cgccagtcta caaaatggcg
    12241 atggactgga aactggcgtt accgctgcac cctgaatacc gcaccctgcc gatggtctgg
    12301 tacgttcctc cgctgtcacc gattcagtct tacgctgatg cgggcggttt gccgaaaagc
    12361 gaaggcgtgc tgcctgctat cgaaagcctg cgtattccgg tgcaatatct cgccaatatg
    12421 ttgagtgctg gcgataccgg tccggtactg cgggcgctga aacggatgat ggcgatgcgc
    12481 cactatatgc gttcacaaac cgtggaaggc gttaccgata ctcgtgccat cgacgaagta
    12541 ggcctgaacg tcgcccaggt cgaagagatg tatcgctacc tcgccattgc caactatgaa
    12601 gaccgttttg tcattccaac cagtcatcgg gaaatggcgg gcgatgcctt tgctgaacgc
    12661 aacggctgcg gttttacctt tggcgacggt tgccacggct cggacagcaa attcaacctg
    12721 ttcaacagta gccgtatcga tgccatcaac atcaccgaag tgcgcgacaa agcggagggc
    12781 gaataatgca gatcctcaaa gtgatcggcc tgttgatgga gtatccggac gagctgttgt
    12841 gggaatgtaa ggaggacgcg ctggcgttga tccgccgcga cgcaccgatg ctcaccgatt
    12901 ttgcactcga cctgctcaac gcgccgctgc tggataaaca ggccgaatgg tgtgaagtgt
    12961 ttgaccgcgg gcgcaccacg tcgctgctgc tgtttgaaca tgttcatgcc gagtcccgcg
    13021 atcgcggcca ggcaatggtg gacctgctgg cggagtatga aaaggtcggt ctgcaactga
    13081 attgtaggga actgcccgat tatctaccgc tgtatctgga gtatttaagc atacttccgg
    13141 acgatcaggc gaaagaagga ttactcaacg ttgcgccgat cctcgccctg cttggcggac
    13201 gcttaaaaca acgcgaggca ccgtggtacg cgttatttga cgccctgttg caactggcag
    13261 gaagccctct atcaagtgac agtgtcacaa aacagattcg cagtgaagag cgtgacgaca
    13321 cccgccaggc gctggatgcg gtgtgggaag aagaacaggt gaagtttatt gaagataacg
    13381 ccacggcgtg tgacagctcg ccgcttaatc aatatcagcg acgctttagc caggatgtcg
    13441 cgccgcagta tgtcgacatc agtgcgggag gtgggaaatg attcagtatc tgaacgtctt
    13501 tttttacgat atctacccgt acttgtgtgg cacggttttt atccttggaa gctggctgcg
    13561 ctacgattac gggcagtata cctggcgtgc cgcctccagc cagatgctgg ataaacgcgg
    13621 gatggtgcta tggtcgaact tgttccatat cggcattctg gggatcttct tcggtcatct
    13681 gttcggcatg ttaacgccgc actggatgta cgcgtggttt ctgccggtgg cggtaaaaca
    13741 gcagatggcg atgatcctcg gcggaatatg cggggtattg acgctgattg gcggtgcggg
    13801 gctactggtg cgtcgtttaa caaatccgcg tatcagggcg acgtcttctg gtgcggatat
    13861 cctgatcctc gccattttgt taattcagtg cattcttggg ttaaccacca ttcctttttc
    13921 agcacaacat ccggacggca gtgaaatgat gaagctggtg ggctgggcgc aaagcattgt
    13981 cactttccag ggcggtgcat ctacgcatct tgatggtgtc gcctttatat tccgcgttca
    14041 tctggtgctt ggaatgacca tcttcctgct cttcccgttc acccgactgg tgcatgtctg
    14101 gagcgcgccg tttgagtact ttactcgtcg gtatcagatt gtgcgttcgc ggcgttaatt
    14161 ctcacgcggg ccggacagcc cttcgctctc cggccacttg caggctatga tagttctcca
    14221 gccacaggag aacattaatg aaaccgcagg tttaccacgt cgatgctttt acctctcaac
    14281 cgtttcgcgg caattctgcc ggtgttgttt tccccgccga taatctcagt gaagcgcaaa
    14341 tgcagcttat cgcccgcgag ttaggtcatt cggaaactgc ttttctgctg cacagcgacg
    14401 acagcgatgt gcgcatccgc tactttacgc caacggttga agttccgatt tgtggtcacg
    14461 cgacagtagc tgcgcactat gtacgtgcaa aggtgttagg tttaggaaat tgcacggtct
    14521 ggcaaacatc gctggcagga aaacatcgcg tgactatcga aaagcacaac gatgattatc
    14581 gtatttcgct ggaacaaggt acgccgggct ttgagtcacc gctggaaggt gaaacacgtg
    14641 cggcgattat caacgcgctt catctcactg aagacgatat tctgccaggc ttgccaattc
    14701 aggtggcaac cacaggacac tcaaaagtga tgatcccact gaaaccggaa gtggatatcg
    14761 acgccctttc gcctgacctt aatgcgctga ccgctatcag caaacagatt ggctgcaatg
    14821 gtttcttccc gttccagatc cgcccagaca aaaacgaaac cgacggtcgt atgttctcgc
    14881 ctgccattgg tattgtggaa gatccggtga ctggcaatgc caatggcccg atgggtgcat
    14941 ggttagtaca tcacaacgta ttgccccacg atggcaacgt gttgcgtgtt aaaggccatc
    15001 aggggcgcgc attagggcgt gacggcgtga ttgaagtgac ggtgacaatt cgtgataacc
    15061 aaccggagaa agtcaccatt tctggcgtgg cggtgatttt gtttcatgcc gaatgggcaa
    15121 ttgaactctg aaaccgttgc ctgatgcgct tcgcttatca ggtctacaac cgggctaatg
    15181 taggccggat aaggcgttta cgccacatcc ggcaacctga cataaattat tttcccgcct
    15241 ccgggtgcgt atcaaacgcc gccatcacca gcgctaactc atccacagta aagccatgtt
    15301 tcgcatcatc tacgcccaga ccaaactgtt cctgcatcac ggcatataac gacgccacat
    15361 ctggtagatt tcgctgctca actgcgtgcc cattttcata atgggtaaaa tgaaaattgg
    15421 tcagcgttag cttgccaccg tccggcaaat ggcgacacat cagcaaatga tggcgaaaat
    15481 gcgactgcgg ccagtgcgcc gaccagaaat tgcccatcac ataatcgctt tgttgctgct
    15541 cgcagagatc aaaacggtac atcgactgcc agtgctgatg atgattaaac tgcaacaccc
    15601 aatcatcacc ctcttgcaac aaccgatact ctccgtgtgg cgtggtttgc atgatatccg
    15661 gcattaaacg aatcggcgcg gttagcgtct gcccaccgaa accgacatca gcaatccatt
    15721 tttccccttc cagttccacc aacagcaaac gatgggtgcg cggcggtaat gctggcggat
    15781 ttgataacac tacgcgccct aacaagctgc gaacgttaaa ccccagctcg cgtaacaccc
    15841 gctcaaacac gccattctgc tcaaaacagt aaccaccacg acgggctatc accagtttct
    15901 cttccagcga ttgatcatca agctgtattt ccctcggcag caaaacgtcg aggttttcaa
    15961 acggaatggt gcaattgtgt ttcaggtgca atgcacgaag cgtatcaata ttggccgcag
    16021 cagctcccga ccagttaata cgggcaaaat aatgattcag aatgggcgtc atcaggcgtt
    16081 tcccttagtg gttttctcaa cttataacct ggaatagacg ccgcaccggc tattttgtgc
    16141 gatcgcggac atgcatcacg ttcgcaaatc gccaatcccg ttaatttcgt tacttattgc
    16201 gttacgagct acgcttttaa aacacccttc atagcgaggc gatatcgtga gccaattcat
    16261 tcccgtcgaa ttacaccatg ccagccgtct gttaaatcat ggcccaactg tcttgatcac
    16321 cagtttcgat gagcaatccc agcggcgtaa cattatggct gcagcctggt caatgccggt
    16381 ggagtttgaa cctccacgcg tggcgattgt ggtagataag tcgacatgga ccagggagtt
    16441 gattgagcgt aacggtaaat ttggcatcgt tatcccaggc gttgcagcga ctaactggac
    16501 gtgggcagtg ggaagtgtat ctgggcgtga agaggataaa ttttattgct atggcattcc
    16561 ggtggtgaaa ggtccggtgc ttggtttgcc acttgttgaa gagaaatgtc tggcgtggat
    16621 ggagtgccga ttgctaccag cgacttccgc gcaagagaaa tacgacacgc tgtttggcga
    16681 agtggtatca gcagcggcag acgcacgggt atttgtcgaa ggccgctggc agtttgatga
    16741 tgataagctc aatacgttgc atcatttagg cgctggcacg tttgttacca gcggcaagcg
    16801 agtcaccgcg ggctaaagaa tcatatgcag caaccaagtt gcttaagcaa tgtttcaata
    16861 tgagttaaaa gcatatttta tctcacgatt gcatattttt agtgaagatt aatttatcct
    16921 caccaacatc atagtaattg cgtattatag cttcattgat aaagccctgt tatgtataaa
    16981 atgcgcgggc ggcactaaaa tcgacatagc tggaggtctc tactaaaagt agtcttgcct
    17041 gtttaattgt gagctccttt tctatttcgt taatcagcag atgtcccact ccctggcgct
    17101 gatgggaagg actgatccag tgcattaata aattccagac atcatttgtc atcacttcag
    17161 gagcgcaata ggcaatccct gccagtccta agtgttctgc actaaaccat aaatcggcag
    17221 attgacctga gaaataatgt gttagtgttt tttgtacatg atgaaggcca tcttgattaa
    17281 attgtccact ggcaagcagc atatccagta atggttcaac atctgaaggt ttgactggtc
    17341 gtataatcat ggaattcctt atctattgat gaatgtttga aaattgtttg cgtggaacat
    17401 ggttaatctg agccaatatt tttgagaaag aatagatggg tatttatata agcaaagaag
    17461 ccatctcttt aaatatcaat taaagtataa cattaacacc agacttcatt gcgacaggac
    17521 acgacaaagc acttttgcgg ttttttcagg gtgcaattac aaaaattgaa tcccttatgg
    17581 actaatgtga gaaaattccg gggcatgact attttgatat agtggacaat caagcgtctc
    17641 gaattaacaa tgacttttca gatgctaaac catgcaattg gaagtatgtc ggatatagga
    17701 caaca
//
LOCUS       DAZGQL010000099        17684 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.099, whole genome shotgun sequence.
ACCESSION   DAZGQL010000099 DAZGQL010000000
VERSION     DAZGQL010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17684
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.099"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(72..2519)
                     /gene="glgP"
                     /locus_tag="ACHKIR_003993"
     CDS             complement(72..2519)
                     /gene="glgP"
                     /locus_tag="ACHKIR_003993"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HFO9991669.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNDLQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYERQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(2538..3971)
                     /gene="glgA"
                     /locus_tag="ACHKIR_003994"
     CDS             complement(2538..3971)
                     /gene="glgA"
                     /locus_tag="ACHKIR_003994"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HFO9991670.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(3971..5266)
                     /gene="glgC"
                     /locus_tag="ACHKIR_003995"
     CDS             complement(3971..5266)
                     /gene="glgC"
                     /locus_tag="ACHKIR_003995"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HFO9991671.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(5284..7257)
                     /gene="glgX"
                     /locus_tag="ACHKIR_003996"
     CDS             complement(5284..7257)
                     /gene="glgX"
                     /locus_tag="ACHKIR_003996"
                     /EC_number="3.2.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HFO9991672.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            complement(7254..9440)
                     /gene="glgB"
                     /locus_tag="ACHKIR_003997"
     CDS             complement(7254..9440)
                     /gene="glgB"
                     /locus_tag="ACHKIR_003997"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HFO9991673.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVRTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPVYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNVGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(9713..10816)
                     /gene="asd"
                     /locus_tag="ACHKIR_003998"
     CDS             complement(9713..10816)
                     /gene="asd"
                     /locus_tag="ACHKIR_003998"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFO9991674.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            11008..11601
                     /gene="yhgN"
                     /locus_tag="ACHKIR_003999"
     CDS             11008..11601
                     /gene="yhgN"
                     /locus_tag="ACHKIR_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HFO9991675.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(11658..12998)
                     /gene="gntU"
                     /locus_tag="ACHKIR_004000"
     CDS             complement(11658..12998)
                     /gene="gntU"
                     /locus_tag="ACHKIR_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFO9991676.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(13002..13529)
                     /gene="gntK"
                     /locus_tag="ACHKIR_004001"
     CDS             complement(13002..13529)
                     /gene="gntK"
                     /locus_tag="ACHKIR_004001"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFO9991677.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(13668..14663)
                     /gene="gntR"
                     /locus_tag="ACHKIR_004002"
     CDS             complement(13668..14663)
                     /gene="gntR"
                     /locus_tag="ACHKIR_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HFO9991678.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSKSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(14887..15582)
                     /gene="yhhW"
                     /locus_tag="ACHKIR_004003"
     CDS             complement(14887..15582)
                     /gene="yhhW"
                     /locus_tag="ACHKIR_004003"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HFO9991679.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASISDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(15705..16742)
                     /locus_tag="ACHKIR_004004"
     CDS             complement(15705..16742)
                     /locus_tag="ACHKIR_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFO9991680.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITNGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAK"
     gene            17076..17564
                     /gene="yhhY"
                     /locus_tag="ACHKIR_004005"
     CDS             17076..17564
                     /gene="yhhY"
                     /locus_tag="ACHKIR_004005"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFO9991681.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGTVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
BASE COUNT         4345 a         4811 c         4420 g         4108 t
ORIGIN      
        1 tgtatagaaa taccgtgagt tccactctgt attacttcag cggaacacac ggtatttagc
       61 catatttgaa cttacaatct caccggatca atatgccaga tatgatcggc gtactctttg
      121 atcgtacggt cagaagagaa gtagcccata ttggcaatgt tcagcatcgc ttttgcggtc
      181 cactcttcct gacgctcgta gagttcgtcg actttatcct gacaatcgac atagctgcga
      241 taatccgcca gtacctggta gtgatcgccg aagttgatca gcgaatcgac cagatcgcga
      301 tagcgacccg gatcttccgg actgaataca ccgctgccga tttgcgtcag cacctgatgc
      361 agctcctcat ctttctcgta gtattcacgc ggtttgtagc cctgacgacg cagttcttcc
      421 acttcttccg ctgtgttacc aaagataaag atattgtcag caccgacatg atccagcatc
      481 tcgacattcg caccgtccag cgtaccgata gtcagcgcgc cgttaagcgc aaacttcatg
      541 ttactggtgc cggaagcttc cgtccccgcc agcgaaatct gttcagacag atcggccgcc
      601 ggaatgatca actgcgccag gctaacactg tagttcggga tgaacacgac tttcagcttg
      661 tcgccaatct gcggatcgtt gttgatcact ttcgctacgt cattgatcaa atgaataatg
      721 tgcttcgcca tgtaataggc cgaagccgcc ttaccaccaa aaatattcac gcgcggtacc
      781 cacttcgcat ccgggtcggc cttgatgcgg ttatagcggg tgatcacgtg caacacattc
      841 atcaattgac gtttgtattc gtgaatacgt ttgatttgca catcgaacag cgctttcgga
      901 tttaccacca cattcagctg ctgggcgata tactccgcca gacgcttttt gttctccagc
      961 ttcgcctgat gcaccgcgtg attaaccatt gggaaatcac agtgttgttg cagatcatta
     1021 agcaggctaa ggtcggtgcg ccagttgcgg cccaggtgtt cgtccaacac ggctgaaagc
     1081 gatgggttcg ctaccgccag ccagcgacgc ggcgtcacac cgttggtgac gttggtgaaa
     1141 cgacccggga agattttcgc aaagtcggca aatagcgatt gcaccatcag gttagagtgc
     1201 agctccgata caccgttaac tttgtggctc accacaaccg ccagccaggc catacgcaca
     1261 cgacgaccgt tggattcatc aatgatcgac gcccgtccca gcagatcggt atcgttcgga
     1321 tactgttcct gcaaggtttt caggaaatag tcgttgattt caaagatgat ctgcaggtga
     1381 cgcggcagaa ttttacccag catatcgact ggccaggttt ccagcgcctc gctcattagc
     1441 gtgtggttgg tgtaggagaa gacctgacaa cacacttcaa acgcgtcgtc ccagctaaat
     1501 tggtgctcat cgatcagcag acgcatcatc tcaggaatcg acagcaccgg atgagtatcg
     1561 ttgaggtgaa tcgcaatttt atccgccagg ttatcgtagg ttttatgcaa ctgataatgg
     1621 cggcttaaaa tgtcctgaat ggtcgaggaa accaggaagt attcctgacg cagtcgcagc
     1681 tcacgcccgg agtaggtgga gtcgtccgga tacagtacgc gagatacgtt ctcggagtgg
     1741 tttttatctt ccactgccgc gaagtagtcg ccctggttga atttaccgag gttaatttcg
     1801 ctactggctt gggcactcca caaacgcaac gtgttggtcg cgtcggtgtc gtaacctggg
     1861 attatctgat cgtaagctac tcccagaatc tcttcggttt caatccagcg cgttttttta
     1921 ccttcctgct gaatgcgacc gccaaaacgg actttatagc gcgtgttgtg gcgtttgaat
     1981 tcccacgggt taccgtattc cagccagtag tcaggcgact ctttctggct accgttaacg
     2041 atgttctgct tgaacatacc gtagtcatag cggatgccgt aaccgcgccc cggcaaccct
     2101 aacgtcgcca gagaatcgag gaagcaagcc gccagacggc ccaggccacc gttaccgagg
     2161 cccgggtcat tttcttcatc aatcagctct tcgagattta atcccatcgc ttccagtgcg
     2221 ccctgtacat cttcgtaaat tcctagcgac aacatggcgt tggaaagcgt acggccaatc
     2281 aaaaactcca tcgacaggta gtaaacctga cgagtttctt gcgacaactg ggcacggttt
     2341 gagcgtaacc agcgctccac gagacgatcg cgcacagcaa ataacgttgc gttcagccat
     2401 tcatgtttat tggcgacgac cggatccttg ccaatcgtaa acatcagctt gtaagcgata
     2461 gagtgcttaa gagcttctac gctaagcgtg ggcgatgaat atgtaaacgg agcattcata
     2521 taggcgtttc ctgaaaacta tttcaagcga tagtaaagct cacggtacga cttcgccgcg
     2581 acctgccagc taaaatccat tgccatagcc tgacgttgca caaaccgcca cagtgaagga
     2641 cgggaccaca gtacaaaagc acgtcgaata gcccgtaaca gcgaccaggc attactatct
     2701 tcaaagacaa acccactggc gacgccatct gcaaggttct caagagaaca gtcagaaacc
     2761 gtatcagcaa gcccaccggt gcgccgcact aacggcagcg taccgtactt caatccataa
     2821 agttgcgtta agccgcacgg ttcaaaacgg ctgggcacca gaatgacgtc cgcgccgccc
     2881 ataatgcgat gcgaaaatgc ttcgtgatag ccaatctgaa cgcccacctg gccggggtat
     2941 tccgctgccg ccgcaaggaa accttcctgc agcaccggat cgcccgcgcc gagtagcgcc
     3001 agctgcccgc cctgctccag aagaccaggt aaggcttcca gcaccaggtc gagacctttc
     3061 tggctagtca gacggctcac cactgcaaaa agcggcactt tatcgtcaac cttaagcccc
     3121 attgcgattt gtaactggcg cttattttcc gctttatctt ccaacgtatc gcgggtgtaa
     3181 cgcgaggcca acagtaagtc cgtctctgga ctccagattt tctcgtccac gccgttcagt
     3241 acgccggaaa gacgcccttc acggtgacgc tgttgcaaca gaccttccat accgtaggca
     3301 aactgcggtt cggtgatctc gcgagcgtag gttgggctga ctgccgtaat gtgatcggca
     3361 tagtacagac cggccttcag aaaagagatt tgtccgttga attccagccc atgaatatta
     3421 aagaatgacc atggcaattg gatgtcattc atgtgatgtg cataaaacat gccttgatag
     3481 gccaggttgt gcacagtaaa caccgacttc gccggacgcc cgcgcgccgc cagatacgca
     3541 ggcgcaaggc ctgcatgcca gtcgtgcgca tgcaccacat caggacgcca gaatgggtca
     3601 agcccgctgg ccatttctgc cccaacccac cccagcagcg caaaacgcaa tacgttgtcg
     3661 gtataggcaa ataagttggt atcgtgatac gggcttcccg gacgatcata gagatgcggc
     3721 gcgtcaatca ggtatatgcc aaccccgttg taatgaccga acaacagcgt gatatgtccg
     3781 gcgaaggtat cacgacggga tactacctgc gcatcggtca cgccacggcg aatatcggga
     3841 aatgcaggca acagtacgcg agcgtcaacg ccgtctgcga tttgtgctgc gggtaatgcc
     3901 ccaataacat cagccagacc gccggtttta agcagcggga acatctctga acatacatgt
     3961 aaaacctgca ttatcgctcc tgtttatgcc ctaacttccg tagcatttcg cgcgttacca
     4021 gcacgatgcc ttcttctgaa cgatagaaac gacgtgcatc ttcctctgcg ttttcaccaa
     4081 tcaccatgcc ttccggaata acacaagcac gatcgatgac gcagcggcgc agacggcacg
     4141 agcgacctac ccatacttcc ggtaacaata cggcggaatc aatgttgcag aatgaattca
     4201 cgcgaacgcg cgagaacaga acggactgca ccaccaccga accggagatc acacaaccgc
     4261 cggataccag tgagttaagg gtcatcccat ggctaccgga gcgatcctgc acgaatttcg
     4321 ctggcggtaa tgattcattg taggtgcgaa ttggccaatt gcgatcgtac atatccagtt
     4381 ccggcaccac agaggccaga tcgaggttcg ctttccagta agcttccagc gtacccacat
     4441 cgcgccagta cggctcggca tccgggtcgg attgtacgca agagagcggg aacgggtgcg
     4501 cataggccag accggcttcg gtgatcttgg gaatcaaatc tttgccaaag tcgtggctgg
     4561 agttctcatc gcgatcgtct tcttccagca gttcatacag atagtcggcg tcaaagacgt
     4621 agatacccat actcgccaga gatttgctcg gatcgttcgg cattgacggt gggttagcag
     4681 gtttttcaac gaattcgata attttatcgt tctcatcaac cgccataacg ccaaatgcgg
     4741 aggcttcttc aatcggtact ggcatacaag caacggtgca acgtgcgcct ttttcgacgt
     4801 ggtcgataag catacgcgag tagtcttgct tgtagatatg gtcgcccgcc aggatcacca
     4861 cgtattccgc tttataacgg cggataatat cgaggttttg ggtaactgca tctgcggtgc
     4921 cgcgatacca gttttcccct ttcattctct gctgtgctgg cagcagatcg acaaactcgt
     4981 tcatttcttc attgaagaat gaccagccgc gctgaatgtg ctgcaccaga gtgtgggact
     5041 ggtactgggt gatcacgccc atacgacgga tcccggagtt gatgcagtta gacagcgcaa
     5101 agtcgataat gcggaactta ccgccgaagt gtacggccgg ttttgctcgc ttattggtta
     5161 aatccttcag gcgggtacca cgtcctcccg ccagtatcag ggcaacagat ttcaatggca
     5221 gctggcgcgc caacattaag tgatcgttct tctctaaact aaccatgact aactcctttt
     5281 ttatcatctc tggaacacac acaatccgtg tgcaggtccc tgccagacag ccgtaatcac
     5341 tgggttatcc tctccagcga atgggggaat ggcgtgccac tccccagcag gtaaaacaat
     5401 ctctgttacc tcaagcgtgg cgttaattgc gatcaaaaag cgatccgaga gcagaatttg
     5461 cagctgtttc ggcccgtttt gccactcatc cgtgcttaaa ggttgagcat atcgatttag
     5521 ccaacggaca ttgccgtcgc cttcttccca ccagcgattc tccaccaaag cgggaatgcg
     5581 cttgcgcaga tggattaacg cggcggtaaa tgcggttaaa ccactgcttg cctgcgacca
     5641 gtccaaccag gttaattggt tatcctgaca gtaggcattg ttattgccat gctggctgtg
     5701 accatgttcg tcaccggcca gtaacatcgg cgtaccctgg gagagcaaca acgttgttaa
     5761 cagggcgtga atgctgtcgc gacgccgttc aaccaggtca agagtaccgc ctaacccttc
     5821 tttaccatga ttgttactgt aattgttgtt ggtcccgtcg cgattttctt ctccgtttgc
     5881 ttcattgtgt ttatggttga agcaaacgca gtcgcgaagc gtaaaaccgt catgcgcggt
     5941 gacgagatta atcgcggcac tcggcagacg accattacgt ttaaaaacat cgctggaggc
     6001 agcaaaacgc ccggcaaacg cccccagagg caaatcataa tgaagccaga aacgacgggc
     6061 agcatcgcgg aaatgatcgt tccactcggc aaacagcggc gggaaatttc ccacctgata
     6121 accaccagga gcaatatccc acggttcagc aattaacttc acctgcgaga gcaccgggca
     6181 gttctggata gcggtaaaca acggcgcatc ctgacggaac tctggcgtac ggcccatgac
     6241 tgccgccaga tcaaagcgga aaccatcgac gtggcaggtt tctacccaat aacgcaggca
     6301 ggcgctggca taatccacca ccgccggatg actcaaattg agcgtgttgc cacaaccggt
     6361 ccagttgtga taatcgccgt cttctcttat ccaataatag ctacggttgt cgatcccacg
     6421 cagagagaat aacgggccgt cgaggtccag ttccgcacta tggttgagca cgatatcaag
     6481 aatgacttcg atacccgctt tatgcagtgc tttgattgca tcgcgaaact catccagcgc
     6541 cgtttctggg gagcaggcat acgccggatg cagcgcaaac atcgccaccg ggttataacc
     6601 ccagtagtta cttagaccca tacgttgcag gcgtggttcg ctggcaaact gcgccaccgg
     6661 cagcagttcc agcgcggtaa tgcccaactg tttcaaatag ttgatcatca ccggatgccc
     6721 cagggcttta taagtgccac ggatctcgac cgggatctcc gggtgcaggt acgttaatcc
     6781 tttgacatgg gcttcataaa tgatggtact gccccacggc gtgcgcggcg gagcatcatc
     6841 ctcccagtca tagtgatcaa ccaccactac gcatttcggc gcaatggcgg cgttgtcgcg
     6901 atagtcaggt tcattatgac cggcgtgcag cagcgggtta tctttaaatt ccccgtcaat
     6961 ttgccgcgcg caaggatcaa tcaacaactt cgccgggtta aagcgatgcc cctcggcggg
     7021 ttgccagggg ccatgaacgc gataaccata acgcaaaccc ggacgcgcat ccggcagata
     7081 accgtgccaa atgtcgccac tgtgccctgg caagtcatag cgatgttcct ggccattggc
     7141 gtcaaagaca cacagttcta cccgctcggc atgagcggag aaaagtgtga agttgacgcc
     7201 ctgaccgtcg taatgcgcgc cgaggggtgc gggtttgcca atggcgagtt gtgtcattct
     7261 gcctcccgaa ccagccagat agtggccagc ggtggtagtg tcaggcttaa tgaatgctga
     7321 cgaccgtggc tggcaatctc atcgctgtgt accgtgccgc cattgccgac attactgccg
     7381 tgatagtgca tggaatcggt attgaggatt tcacgccatt tgcccggctg gttaatgccg
     7441 aagcgataat catgacgcgg caccggagta aagttactgg caacgatgat ttcgttaccc
     7501 tctttatcgc gacgcacaaa gatcagcacc gagcgttctt tgtcatccac caccagccat
     7561 tcaaaaccgt acgggtcaaa atccagttca tgcatcgctt tatggtggcg gtaggtgagg
     7621 ttcagatcgc gcaccagacg ctggacaccg tggtgccagt tatcgccgcc ttccaacaga
     7681 tgccagtcga ggctggcgtc atggttccac tcgcggccct gggcaaattc gttccccatg
     7741 aacagcagtt tcttgcccgg gaatgcccac atccagccgt agtaggcgcg caggttagcg
     7801 aatttctgcc atgcgtcgcc cggcatccgg tcgagaatcg attttttacc gtggaccact
     7861 tcatcatgcg acaacggcag gacgaagttt tcagtgtagt tgtagagcat cccgaaggtc
     7921 agtttatcgt gatgatactg acgataaacc gggtcgagct tcatgtagtc cagggtgtca
     7981 tgcatccagc cgaggttcca cttgtaccag aagcccagac cgcccatatc ctgcggacga
     8041 gaaacgccag ggaaatcggt agactcctca gccattgtca ccgcaccgga aacctgctca
     8101 ccaagaatac ggttggtatt acgcaagaat tcaatcgctt caagattctc gcgcccgcca
     8161 aattcgttcg ggatccactc cccctcttta cggctgtagt cgcgataaat cattgacgct
     8221 accgcatcga cgcgcagcgc atcaatacca aaacgttcaa tccagtaaag cgcgttaccg
     8281 acaaggaagt tactgacttc acggcgacca tagttgtaga tcagcgtgtt ccagtcctga
     8341 tgatagcctt cgcgcggatc gctgtgttca tacaagttcg tgccatcaaa ttcggcaagc
     8401 gcaaagtcat cggtcgggaa gtggcctggc acccagtcga gaatcacgtt cagaccagct
     8461 gcgtgtgcgg catcaatgaa ataacggaag tcgtcgcgag taccaaaacg gcgggtcggt
     8521 gcatacagac cggttggctg ataaccccaa ctgccatcga agggatgctc gttaatgggc
     8581 agtagttcga ggtgggtaaa gcccatccat ttagcataag gcaccagttg atcggccagc
     8641 tcgcggtagc tcaaccagaa attgttgtcg gtgtggcgac gccaggagcc cagatgaact
     8701 tcataaatag agattggcgc atcaaactga ttcgcttttt tgcgctcttc agtccgtaca
     8761 accttttccg gcagcccgca aataagagac gcggtttccg ggcgcatttg cgcttcgaag
     8821 gcataagggt cggacttcag acgcaagttg ccattggcat caatcatctc gtatttatag
     8881 agctgaccgt tatgcgcccc agggataaac agttcccaga tgccgctctc tttacgcagg
     8941 cgcatcgggt gacggcgacc gtcccagtag ttgaattgcc caaccaccga gacccgacgg
     9001 gcgtttggag cccagacaga gaaacgcgta cctgtgacgc catccatagt atctgcatgc
     9061 gcgcctaagg tttcatacgg gcgcaggtga gtaccttcag ataatagcca ggcatccatt
     9121 tcctggatta gcggaccaaa acggtaagga tcatcaatca ggttttgctg accatgccag
     9181 acaacagcca actgatagcg gaaaaaattc ttacgtcgcg gaatgacgcc gctaaagaat
     9241 ccccgtgagt cgagacactc cagttttgcg agtttgcgcc cggttttcgg ttcaatcacc
     9301 cacacatcgg tagcgtcggg taaaagggca cggacttcca gtcccgcggt ggttttatgc
     9361 attcccagta cggaaaaagg atccgcaaaa tggcctgcaa ttagcgcgtt aatcacgtct
     9421 ctatcgatac gatcggacat gcttgtcttc ctgttttatt gtgtcacccc atccaattgg
     9481 atttttgact tctggttgtg acattttttt gacctgaacg gcgcagcact ctgtgcatcc
     9541 tctctgcgtc gtcctcactt caggtaaggc tgtgaatact catgtattca gccaccctta
     9601 aagaatagcc aatgctctat ttaactcccg gtaaatcatg aaacatctgc gcttactcct
     9661 gtattacgca ctaacagggg cggcatcgcg ccccagattt aatgaataaa gattacgcca
     9721 gttgacgaag catccgacgc aacggctccg cggcccccca cagcagctgg tcacccacgg
     9781 taaaggctga caggaactcc ggtcccatat tcagcttacg caggcggcct accggcgtgg
     9841 tcagcgtgcc ggtaacggca gctggggtta gctcacgcat agtgatttcc cgatcgttcg
     9901 gaacgacttt cgcccacgga ttgtgtgcag ccagcagttc ttccacggtc ggaatagaca
     9961 catctttttt caatttaata gtgaatgcct ggctgtggca gcgcaatgcc ccgacacgca
    10021 cacataaacc atctaccgga attacggaag atgtgttgag gatcttgttg gtttccgcct
    10081 gccctttcca ctcttcgcgg ctctgaccgt tatcgagctg tttgtcgatc cacggaatca
    10141 ggctacccgc cagcggcacg ccaaagttat ccaccggcag ctcaccgcta cgggttaagg
    10201 ttgtgacttt gcgttcgata tcgagaatag cagaggacgg ggtcgcgagt tcatctgcca
    10261 catggccata cagatggccc atctgggtta ataactcacg catatgtcgc gcaccaccgc
    10321 cggaagcggc ctggtaggtt gcaacggaca cccaatcaac aagatcattg gcgaataaac
    10381 cacccaacga catcaacatc aggcttacgg tacagttacc gccaacaaaa gtcctgatgc
    10441 cattatttaa tccatcggta atgacgtcct gattgacggg gtcaagaatg atgatggcat
    10501 catctttcat gcgcagagac gatgctgcgt caatccagta accttgccat ccgctttcac
    10561 gaagctttgg atagatttcg ttggtataat cgccgccctg acaggtcaca atgatatcga
    10621 gggcctttag cgcctccaga tcaaaggcat cctgaagtgt gccagtggtt ccgccaaaag
    10681 acggcgcagc ctgaccaagc tgagaagtag aaaagaagac agggcgaatg gcgtcgaagt
    10741 cgcgctcttc aaccatgcgt tgcatgagaa cggagccgac cataccgcgc cagccgataa
    10801 aaccaacatt tttcataagc gttttttcct gcaaagatgt gtgctgtata aatgtgccgg
    10861 tctcctcttg gcacatcttt caccatacaa aaagcagcca aagtcgcaag tgaaattaat
    10921 caatgatagc gaagccatca gtaatgcgac ttatcctgct ttgttagcac gcagaaagtc
    10981 cgcggcaatt atcagggaat ttgagttatg aatgaaatca tttctgcagc agttttattg
    11041 atcctgatta tggatccgct cggaaaccta cctattttca tgtccgtact gaaacatact
    11101 gaaccgaaaa gacggcgggc aatcatggtg cgagagttgc ttattgctct cctggtgatg
    11161 ctggtgttcc tgtttgcggg tgagaaaatt ctggcatttc tcagcctgcg agcagaaacc
    11221 gtctccattt ctggcggcat cattctgttt ctgatcgcca ttaagatgat tttccccagc
    11281 gcttcaggaa atagcagcgg gcttccggca ggtgaagagc cgtttatcgt gccgttggca
    11341 attccgttag tcgccgggcc gactattctc gccacgctga tgttgttgtc tcatcagtac
    11401 ccgaatcaga tgggacatct ggtgattgct ctgctgctgg cctggggcgg cacctttgtc
    11461 atcctgctac agtcttcgct atttttacgt ctgctgggcg agaaaggggt gaacgcactt
    11521 gaacgcctga tgggattgat tctggtgatg atggcaaccc agatgttcct cgacggcatt
    11581 cgaatgtgga tgaaggggta acgaggagag tttttcgaaa agagcccggt agcgtcagaa
    11641 ctaccgggta aacaaactta actcaacagc tggaacgcaa tcatcccaac gatggcaccg
    11701 acagtgccga ggatggtttc catcatggtc caggttttca gcgtttcggc ttcggtcgcg
    11761 ccggtaaatt taccgaacaa ccagaaaccg gcgtcgttaa cgtggctgac aacaatcgaa
    11821 ccaccggcga tacaaatcga cagcgccgcc atttgcgcac cagagtagtt cagttgttca
    11881 atgactggca tcaccagtcc caccgccgtt aaacaggcta cggtggcaga cccctgaatg
    11941 atgcgcactg cagctgccag cacgaagcag gtgatagcaa tcggcaggcc catgccggtt
    12001 aacgcttcgc ccagtgccgg acctacgcca gagtcaacca gcacctgttt gaacacgccg
    12061 cccgcaccaa tcaccagcag aatgatcccc gccggttgca gcgcgtgacc gcaaatctcc
    12121 atcactttgt cttttggcat gccctgacgc attgccaggc cataaatcgc taccagacaa
    12181 gcaaccagaa tcgcggtaaa cggatgacca ataaactcga accattcgta agcggtagag
    12241 ccttctggca caaaacgcgc ggcaatggtt ttcagcccca ccagcaccag cggcaacagg
    12301 atcagcgaca ggctgaatcc gaaagatggc attttacctt cgccgagatg cggttcgctg
    12361 atgtcgtcag gaatatgcag ctcaacgtaa cggctgatga agttacccca cagcggcccg
    12421 gcaataatca ttcccggaat tgccgcacac aggccaatca ggatcatcca gccaaagtcg
    12481 gcgttcatct gcgatgccag cagcattggc gctggccccg gcaccaggaa cgcagccgct
    12541 gccgccacgc ctgcaaataa tgggattacc agcttcacca ggttcgtacc ggtatgacgc
    12601 gccattgaga aagcaacgct aatcagcaga acaatcgcca cttcaaagaa cagcggtagc
    12661 gcacaaacca gccccgcaag gccaatggca taatgcgcgc ggctgtgacc gaaggattta
    12721 agcattttga cggcaatctg atcgactgcg ccggtttcat gtaagatctt gccaaacata
    12781 gctcccaggg cgacaaccac cgccaggaag ccgagggtgc ctcccatccc tttttccatc
    12841 gtcgctgcga ttttatcgag cggcataccg gaaaaaaggc cagcccccat ggacaccacc
    12901 attaaagcca ggaaagcgtg catacgcgcc ttcatgacta aaaacagcag cagtaaaaca
    12961 gaccctactg ctgttaaaac aagcgttaat gtagtcacta cttatttgcc ttttttaata
    13021 acctcaatgg tgcttgccac aacaccttcc agcggttgat cgatatccac caccagtaca
    13081 tcggtttcgt ccgcacccgg ctcctgcagc gtttcaaact gcgtcaccaa catttgggtt
    13141 ttaaagaaat ggcctttgcg cgctttcagg cggctttcaa tcacatcaaa atcacctttc
    13201 aggtagatga aagagagatt cggattacct tcacgcagca agtcgcgata gtgttttttc
    13261 aatgcagaac agacgatcag tgacacttta ttagtgcgct gcatagcaaa cgcggcgtcg
    13321 ttcagcgcct gcagccacgg tttgcgatcg tcatcattca gcggttcgcc agacgccatt
    13381 ttttcgatat tgcgccgtgg atggaggaaa tcgccatcaa gaaacgcggc atgaagttga
    13441 tgcgccactt cacttgcgac cgcagatttg ccgctgcccg atacgcccat caagacgtaa
    13501 atgtggtgat catggttagt cgtgctcaaa gtggtgcccc cacaatacaa gaattaaaaa
    13561 tgttacgggt aactgttatc ggtaacattg tccagccgga caatatcaga agcaatatcc
    13621 atccgtgcct taagtgtata agtgtgagct acttcaaatt tgtgggctta aatagatccg
    13681 cccggtgaca aggtgaaacc taaatctaac attttcggtg tcacagattc gccacgaata
    13741 cgcgccagca ggcgttcagc gccaatactg cccatccgct cacgcggcgt caggacgctc
    13801 gccaaccgtg gctccatcac ctggccaatg tcatgaccgt ggaaaccggc aatcgccata
    13861 tcgtcaggaa ctttcaaccc cagacgctga cattcaaacg ccgcgccgac cgccaggtca
    13921 tcattagtac agaacacgcc atccagctgc ggatattccc gccgtgcctg gcgaatcagt
    13981 tcaataccgg aagaataaga agaagattgc tcaaccatca cgctatacgg caccaggcct
    14041 gcatccagca tcgcctgttc atatcccttc tgtttgatga tagtacgttc gtcgagacgt
    14101 gcgccgagat aggcaatgtg gcgatgcccg cgagcaataa tggctgtggt catctggcgt
    14161 gccgcttcaa agttatcaaa accgacggcg atatcgaggc aaggcgactt gctgtccatc
    14221 agttccacca ccgggatccc cgccacttca atcatcttta aggtgcgcgg cgtgtgggta
    14281 cgttcggtga ggatcagtcc gtcgatattc catgagagca tggattcgag acgttcttgc
    14341 tccatttccg gtttataacc gtagtgggcc agcatggtct gataaccgtg cgcgtcggtg
    14401 acgctttcga ttccgcgtaa tacttccgcg aaaacctggt tggtgagaga aggtaacagg
    14461 acgccaatcg cccggctggt ggcgttagag aggatatcgg gtgcgcgatt gggaatataa
    14521 cccagttcat caagagcggc ggcaatcttg ccgcgtagag cgacggaaac ctgctccggg
    14581 ttacgcaaaa agcggctgac cgtcattttg gtcacgccta cacggtcagc cacatcctga
    14641 agtacgggtc ttttcttttt catcgtcctg aaggtacaaa agagatagat tctcttagtt
    14701 taacacggac gcatcagaac cttcccgggg aaaacagtgc ttgtttacaa ttattagata
    14761 tacactaaat caattgcatc gcattgtgct aacgacgcgg caaaaccccg gaagcgtaca
    14821 tcagcacgcg accggagcga gcgaggaagc ctgcaaaaag gcaggcttca aagatgccgt
    14881 tgagctttaa accggcggca gatcgaacag taacacttcg ctgtcgctat ccgcatggat
    14941 ggagattgcc tgctcatccc agattgccag accatcgctg atcgaggctt tcacgccgtt
    15001 aatggtgaca ttgcctttca ccacctggat ccagacgcgg cgttcagcgg caatctgatg
    15061 caccgactgc tcatctttca gcaacgccca gcggtacagt tccatatcct gatgcacttt
    15121 caatgaacca tcacgcgcat ctggcgaaag caccagttgt ttgccctgca ctgcatcgaa
    15181 gcgacgctgt tcataacgcg gcgtaatgcc gttttcttcc ggcatgatcc agatctgata
    15241 caggtgcaga cgctcggtgc tgcttgggtt gtactctgag tgacgaatac ccgtacccgc
    15301 actcataatc tggaactcgc ccgccggaac ctgctcttta ttgcccatgc tgtcctggtg
    15361 ctcaacagta ccttccagca cgtaggtcaa aatttccata tctttatgcg ggtgagtgcc
    15421 gaagccctgc cctgcttcaa tcacgtcgtc gttaatcacg cgcagcgcgg agaagcccat
    15481 aaagttcgga tcgtaatagt tggcaaaaga gaaagtatgc caggagtcca gccagccatg
    15541 atttgcatga ccacgttcat ttgctttgcg taagtagatc atttgtattc gccccctgaa
    15601 tgatttcgat gggcttagtg tggacccgat ctgcctggga tgatagaggg tgaaaattga
    15661 cccctctgtt caaaaaaatt gaactattcg aggggccatc aaacttactt cgcgagagtc
    15721 actgtggagg gagaggcttg ctcaaatccg cgttcaagga tttccagatt ggtaagaact
    15781 tcagattcct tgacgtaatt tggcgcaccg ttggtgatgg tttgatacaa cgcatcataa
    15841 acgcgcccgt aatcgcccat ctccggcttc atctcttctc tgaccgtcac accctcgtca
    15901 ttgacatact ccagcacacc gaccgaatca tccgctgcga atcccggttc gcccggcata
    15961 atattagcct tgaggctggt ttcctgctgg tcgataccgt atttaataaa cgaacctttc
    16021 ttaccgtgaa cgataaattt cggataatcg attttcacca gatggctggt tttgacgatg
    16081 gctttcaagt cgccataaaa cagttgcgct tcaaaggtgt cgtcaggatt ggctttattg
    16141 cgcaggctgc ggatgtcata agcgacgtga tccgggcgac cgaacagaga aataatctgg
    16201 tccatcgtat gcacaccaag gccatagaac gcgccatcct gcggcaaccc aggtttggtt
    16261 tctgccaccg ggcggtaata gtcaaaatgg ctttccactt caacaatctc tcccaacttg
    16321 ccgctttcaa tcgctttttt cgccgtcagg aagcaggaat caaagcgacg attctgatac
    16381 ggcgtgacgg tcagcccttt gcttttcgcc agcgcaaaca gctccttcgc ctgggcaagc
    16441 gtcggggtga acggtttttc gaccagcaca tttttcccgg cttccagcgc gcgtttcgcg
    16501 tattcgaaat ggctgtccgc gtgggtgcag acgacaacca gcttaacatc gggatcgttt
    16561 agcacttcgt cgagatcgct ggtgaaatgg atatgagagt aaatgggagc ctgttcttcc
    16621 ggctttgcat ggcggcgaaa aatatgcgcg acatgccagc tatccttgcg gttaagtaca
    16681 tacggcagat ggtaacgggt ggtgcttttg ccgaagccaa taaaggcgca gttgatgacc
    16741 atgatttcgt cctttttaag gtggttattt acaccttagc gcaaagcgga cgtggttcct
    16801 actggagtgc gcataacgaa cacaagcact tcccgaggat aaattgagaa cgaaagatca
    16861 aaaaaaaagc cagcacccgg ctggctaagt aatactggaa gcaatgtgag caatgtcgtg
    16921 ctttcaggtt ctccgcgagg gtcttcctga tcgcgagaca ataataatca ttctcattcg
    16981 cacttgtcca acactttttg caaaaaaatg catttgactc gcattcgaaa gtcaatgatg
    17041 ttgaaaggga catttacccc aaagaggaca aaggaatgag tgagatagtg atacgccacg
    17101 cagaaacacg ggattacgag gccatcaggc agattcacgc ccagccggag gtgtattgca
    17161 acacactaca ggtgcctcat ccctccgatc atatgtggca ggaacgactc gccgatcgcc
    17221 ccggcatcaa gcaactcgtc gcctgtattg atggaaccgt agtaggtcat ctcaccattg
    17281 atgtacagca acgcccgcgc cgcagtcatg ttgccgattt tggtatctgt gtcgactccc
    17341 gttggaagaa ccgcggcgtc gccagcgccc tgatgcgaga gatgattgaa atgtgcgaca
    17401 actggttgcg ggtagatcgt attgagctaa cggtgttcgt cgataacgcg ccagcaatta
    17461 aggtctataa aaaatttggc tttgagattg aagggactgg caagaagtac gcattacgta
    17521 atggtgaata tgtcgatgcg tattatatgg cgcgggtgaa gtaagatagt gccctttttc
    17581 tgagatggaa aaagggtgtc attcaaaatc ggcatacctt cctttaaggt atttattttc
    17641 ccaatacata tattgagatt taactcataa ttacttccta aagt
//
LOCUS       DAZGQL010000100        17444 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.100, whole genome shotgun sequence.
ACCESSION   DAZGQL010000100 DAZGQL010000000
VERSION     DAZGQL010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17444
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.100"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(187..918)
                     /gene="rlmB"
                     /locus_tag="ACHKIR_004006"
     CDS             complement(187..918)
                     /gene="rlmB"
                     /locus_tag="ACHKIR_004006"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFO9991682.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(1009..3450)
                     /gene="rnr"
                     /locus_tag="ACHKIR_004007"
     CDS             complement(1009..3450)
                     /gene="rnr"
                     /locus_tag="ACHKIR_004007"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFO9991683.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKPKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3489..3914)
                     /gene="nsrR"
                     /locus_tag="ACHKIR_004008"
     CDS             complement(3489..3914)
                     /gene="nsrR"
                     /locus_tag="ACHKIR_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HFO9991684.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4119..5417)
                     /gene="purA"
                     /locus_tag="ACHKIR_004009"
     CDS             complement(4119..5417)
                     /gene="purA"
                     /locus_tag="ACHKIR_004009"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFO9991685.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5521..5718)
                     /gene="yjeT"
                     /locus_tag="ACHKIR_004010"
     CDS             complement(5521..5718)
                     /gene="yjeT"
                     /locus_tag="ACHKIR_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HFO9991686.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5800..6804)
                     /gene="hflC"
                     /locus_tag="ACHKIR_004011"
     CDS             complement(5800..6804)
                     /gene="hflC"
                     /locus_tag="ACHKIR_004011"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HFO9991687.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6807..8066)
                     /gene="hflK"
                     /locus_tag="ACHKIR_004012"
     CDS             complement(6807..8066)
                     /gene="hflK"
                     /locus_tag="ACHKIR_004012"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HFO9991688.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTDPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8152..9432)
                     /gene="hflX"
                     /locus_tag="ACHKIR_004013"
     CDS             complement(8152..9432)
                     /gene="hflX"
                     /locus_tag="ACHKIR_004013"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HFO9991689.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGEGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9509..9817)
                     /gene="hfq"
                     /locus_tag="ACHKIR_004014"
     CDS             complement(9509..9817)
                     /gene="hfq"
                     /locus_tag="ACHKIR_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HFO9991690.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9903..10853)
                     /gene="miaA"
                     /locus_tag="ACHKIR_004015"
     CDS             complement(9903..10853)
                     /gene="miaA"
                     /locus_tag="ACHKIR_004015"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFO9991691.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10846..12693)
                     /gene="mutL"
                     /locus_tag="ACHKIR_004016"
     CDS             complement(10846..12693)
                     /gene="mutL"
                     /locus_tag="ACHKIR_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFO9991692.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTVEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKMVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAVREPVAPLYTPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLALPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12703..14040)
                     /gene="amiB"
                     /locus_tag="ACHKIR_004017"
     CDS             complement(12703..14040)
                     /gene="amiB"
                     /locus_tag="ACHKIR_004017"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HFO9991693.1"
                     /translation="MMYRIRNWLVATLLLLCAPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPSPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTFLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14059..14520)
                     /gene="tsaE"
                     /locus_tag="ACHKIR_004018"
     CDS             complement(14059..14520)
                     /gene="tsaE"
                     /locus_tag="ACHKIR_004018"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFO9991694.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14492..16039)
                     /gene="nnr"
                     /locus_tag="ACHKIR_004019"
     CDS             complement(14492..16039)
                     /gene="nnr"
                     /locus_tag="ACHKIR_004019"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HFO9991695.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADALGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAIGIDVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANTH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSQWLIPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            16038..17177
                     /gene="queG"
                     /locus_tag="ACHKIR_004020"
     CDS             16038..17177
                     /gene="queG"
                     /locus_tag="ACHKIR_004020"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFO9991696.1"
                     /translation="MSEPLDLNQLAQNIKQWGLELGFQQVGITDTDLSASEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACVVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17160..17213)
                     /locus_tag="ACHKIR_004021"
     CDS             complement(17160..17213)
                     /locus_tag="ACHKIR_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9991697.1"
                     /translation="MRFYFYSQAVDESGVTR"
BASE COUNT         3969 a         4967 c         4365 g         4143 t
ORIGIN      
        1 aatattttgt tcaggtacag tttgcagcgc cgtcgccagc gccaacggat tccatgtcat
       61 atattattcc atataaatta agtttaaata ttaataaaat gaatatttgc agtacgtaat
      121 tatcttacca gctatagaca aaaaaaaacc atccaaatct ggatggcttt tcataattca
      181 gaggaactag ctgcgctgac gaaccgcttc aaataagcaa attccggttg caaccgaaac
      241 gttcagggaa gaaacacttc ctgccattgg gatgctgatc aactcatcgc aatgttcacg
      301 ggtcaggcga cgcatacctt caccttccgc gcccatcacc agcgccaggc gtccggtcat
      361 tttgctctga tagagtgtat gatccgcctc gcctgccgta ccgacgatcc agatattctc
      421 ttcctgcaac atacgcatgg tgcgcgccag attagtcacc cgaatcagcg gaacgctttc
      481 tgccgcacca caggccactt ttttcgccgt ggcgttgagc tgtgcggagc gatctttcgg
      541 cacaatcacc gcatgaacac cagcggcgtc cgcgctacgc aggcacgcgc cgaggttgtg
      601 cggatcggtt acgccgtcga ggatcagcag gaacggttga tcgagcgaag cgatcagatc
      661 cggcagatcg ttttcctgat actgccgtcc tggcttcaca cgagcgataa tgccctgatg
      721 cacggcaccg tcgcttttct cgtcgagata ttggcggttt gccaactgga taaccacgcc
      781 ctgagactca agggcgtgaa tcagcggtaa cagacgttta tcttcacggc cttttaaaat
      841 aaagacttcc tgaaaacgtt caggggcgcg ctccagcagg gcctgcactg catggatgcc
      901 gtaaatcatt tcgctcatta atgtactcgt tatttacgtt ttgccgggag cgccacgctt
      961 gcccggccta cggataattc accctcttct gtttgaagag ggtaatgatc actctgccac
     1021 ttttttcttc gccgcacgct tcgctttggt cgctgcggct attttctgcg ttttcgccga
     1081 tggctttttc gcttttctcg cgtctttctt cgctgctttc ggcttcggtt ttttctcgcc
     1141 gcggaaggcg ctgtctggct caaagttgac ctttttgccg acctgacgcc gcttgccgcc
     1201 ttttttgcct gcatcgcctt ttttcgcttt ctcgcgcgcc gttttaccga cgttgcgcgg
     1261 tgcgcgttcg ctggagatca ggctaaagtc gattttgcgc tcgtccatat taaccgcttc
     1321 gacgcgaact tccacgcgat cgcccaggcg ataagtctgg ccgctggatt cgcccatcag
     1381 gcgttgcccc acctggtcaa agcgatagta gtcattgtcc agcgaagaga catggaccag
     1441 accatcaatg aacaagtcat ccaggcgaac aaagaagcca aaaccagtga cgctggaaat
     1501 tacgccttta aagacgttac ctacctggtc gagcatgaag tcacacttca gccagtcagc
     1561 cacatcgcgc gttgcttcgt cggcacgacg ttccgccatc gaacagtgct gacccagttg
     1621 cagcatctct tccatcgaat aatggtagcc gccggtttca gtggtattcc cctgatgccc
     1681 ctgctctttc gccaacagat acttaatggc gcggtgcagc gtcaggtcag gataacgacg
     1741 aatcggcgaa gtaaagtgcg cataggactg caatgccaga ccgaagtgac cacggttttc
     1801 cggatcgtaa atcgcttgtt tcatcgagcg cagcagcatg gtttgcagca tttctgcatc
     1861 aggacggtcg gcaaccgact ccagcagctc tgcgtagtcg cgcggttccg gcttgttacc
     1921 gcccggcagt tccagcccca gctccgccag cactgaacgg aaagaggtaa tcgcttcggt
     1981 gctcggcttg tcgtgaatac ggaacagtgc cggttctttc gctttctcaa cgaaacgcgc
     2041 cgccgagata ttcgccagaa tcatgcactc ttcaattaac ttgtgtgcgt cgttacgctg
     2101 agtctgttcg atacgctcaa tacggcgttc ggcgttgaaa atgaacttcg cttcttcgct
     2161 ctcaaatgag atcccgccac gttcttcacg ggctttatcc agcactttat agaggttatg
     2221 caactcttcg agatgcttaa ccagcggggc atactgctcg cgcagatcct gatcgccttg
     2281 cagtatatgc cagactttgg tgtaggtcag acgcgcgtga gaactcatca ccgcttcgta
     2341 gaatttgtag ccagtcaggc ggcctttcga cgaaaccgtc atctcgcaca ccatacacag
     2401 gcggtctacc tgcgggttga gcgagcacag gccgttagag agcacttccg gcagcatcgg
     2461 gataacctgc gaagggaagt acaccgacgt accacggtta cgcgcttctc tgtccagcgg
     2521 tgttggcgga cgcacatagt aactgacgtc ggcaattgcg acccataaac gccagccgcc
     2581 gccgcgtttt ttctcacagt aaactgcatc gtcaaagtca cgggcgtctt cgccatcaat
     2641 ggtgaccagc ggtaaatcgc gcagatcgac acggcccgct tttgcttctt ccggcacttc
     2701 ttctttcagc ccggcaacct gttgctcaac agcctgcggc cagatgtacg gaatttcatg
     2761 ggtacgcaga gcaatatcaa ccgccatgcc ggtgcccata ttatcgccca gcacttcgac
     2821 gattttaccc accgctttgg tacggcgagt cgggcgttga gtcagttcaa ccaccaccac
     2881 aaagcccatc cgcgcgccca tgatctgatc gggcgggatt aagatatcga agctcagacg
     2941 gctatcgtca ggaaccacaa agccgacgcc cgcttcggtg aagtagcgac caacaatctg
     3001 gctggttttt ggcaccagta cgcggacaat acgcgcttca cgacgacctt tacggtcagc
     3061 gcccagcggc tgagccagca cctgatcgcc atgaatgcag gttttcatct gctcgctgga
     3121 gagatacaaa tcatctttac gcccttcaac tcgcagaaag ccgtagccat cacggtggcc
     3181 aataacggta cctttcacca ggtcgaggcg ttccggcagc gcatagcact gacggcgagt
     3241 gaagaccagt tgaccatcgc gctccatcgc gcgcaggcgg cgacgcaggc cttcaagctg
     3301 ctcttcgcct tcaatgtgca gttctaccgc cagctcatca cggctggccg gtttttcacg
     3361 tttggttaaa tgttcgagga taaattcccg actagggatg ggattcgcgt atttttcagc
     3421 ttcgcgttcc tggaaaggat cttgtgacat ctcggttcct ccgttgtcat ctctgatgaa
     3481 gattttcgtc actccaccag caataattta taaagcggtt gattctcttc aaccaaatcg
     3541 gcaagcgtgt agttatccag ttccgtaaga aaactttgca cggccttaga aagcgcctgt
     3601 ttcaaccgac aggcaggtgt aatgtggcaa aactcactgc tgcaattcac cagcgataag
     3661 ggctccagct cgcgcaccac atcaccaata cgtattgcac tcgccggttt acccaagcga
     3721 atgccgccat tttttccacg aacagcagtc acgtagccgg cacgactaag ttgattgatt
     3781 attttgacca tatgattacg ggagacgccg tagacgtcag tcacttcaga aatactggtc
     3841 atccgccctt ctggcaatga cgccatgtag atcagcgcac gtaatccgta atcagtgaaa
     3901 ctcgttaact gcacatcaac ctcaaaaggg aaatcgggga aaaatacatt tatattgatg
     3961 ataaaccagc cacaagctgt gtcgctaatt tatttcagtt cggggaagga aaaaagcgag
     4021 gatttaacac tgtgtcggat agcgtaatgg cttaaccgac acagcaatgt atcacactgt
     4081 tagcccggca ggcaaaatat ctgccaggcg taccagaatt acgcgtcgaa cgggtcgcgc
     4141 agaatcatgg tttcagtacg atccggacca gtagagatga tatcgatcgg cacaccggtc
     4201 agctcttcaa tgcgcttgat gtagttcagt gccgcctgcg gcaggccgct acgatctttc
     4261 acgccgaagg tggattcaga ccagcccggc atggtttcgt aaatcggctc tacacctttc
     4321 cagtcgtcag ctgccagcgg agtggtagtc acttcacgac catccggcat acggtaagcc
     4381 acgcagagtt taacctcttt caggccatcc agaacgtcca gtttagtcag gcagaagcca
     4441 gacagagagt tcagctgtac cgcacgacga acggcaacgg tgtccagcca gccggtacga
     4501 cgacgacgac ccgtagttgc gccgaattcg ttaccctgct tgcagaggaa ctcgccagtt
     4561 tcatcaaaca gttcggtcgg gaacggacct gcacccacac gagtggagta agctttgagg
     4621 atacccagaa cgtaatcaac ataacgcggg cccaggccgg aaccggtcgc cacgccacca
     4681 gcagtggtgt tggaagaagt tacgtacgga tatgtaccgt ggtcgatatc cagcagcgta
     4741 ccctgcgcac cttcaaacat gacgaaatcg ccacgctgac gcgcctggtc aagcaggtca
     4801 gaaacgtcaa caaccataga agtcaggatg tcggcaacag ccatcgtatc atccagaact
     4861 ttctggtaat caaccgcttc agctttgtag tagttaacca actggaagtt gtgatattcc
     4921 atcacttctt tcagtttttc agcgaaggtt tctttgtcga aaaggtcgcc aacacgcaga
     4981 ccgcgacgtg ccactttatc ttcataagca ggcccgatac ctcgaccggt ggtgccgatc
     5041 gctttcgcgc cacgcgcttt ctcacgcgcg ttatccagcg caacgtgata atcaaggatc
     5101 agcggacatg cttcggacag cagcagacgc tcacgaacgg ggatgccacg gtcttccagt
     5161 tctttcatct ctttcatcag cgcagccgga gacagcacaa caccgttacc gatgatgctg
     5221 gttacattct cgcggagaat acctgatgga ataagatgga gaacggtttt ttcaccgttg
     5281 attacgagag tatggcctgc gttgtgaccg ccctggtagc gtacaacata tttagcccgt
     5341 tcagtcagaa gatcgacgat cttaccttta ccttcgtcac cccattgggt gcccagtacg
     5401 acgacgttgt tacccatttt tcaaaatcac cgtttgctta aaaatggatt ctaccatcgc
     5461 tttttcagag ttacagcact ttttgcactc aaaaatgacc tcgatcagtc tgttttttgt
     5521 tcagccaatc gttttcctca acatgtagta gaccacaacg cccgcaacca caagtccacc
     5581 gccaaaacga cgtaaaatat tatcgggcaa attggtcatc gcagagatca tcttcttcca
     5641 tgccttcggg taaagcatcg gccctaaacc ttccagtacc aaaaccaggg caagcgccag
     5701 ccagattgtc gaattcattc attatcctta tagaaaaaga aaaccaccga catccctgag
     5761 gatgcggtgg ctttattgac ctgtaccgca gtcgttatat taacgcgttg cggaagtcgg
     5821 cgtcttcatg tagcggaaga aatcgctatc cgggctcatg accatcacgt cctgattgcc
     5881 agagaagctg ttctcataag cacgcaggct acggataaat gcgtagaagt ccggatcttt
     5941 actgaatgca tcagcaaaca gtttggctgc ttctgcatcg ccttcaccac gcatgatgcg
     6001 gccctgacgc tcagcttcag ccagcgttct ggtcacttca tagtcggcag tcgcgcgcag
     6061 tttttccgct tcttcctgac cttgtgaacg gtgacgacgc gctaccgctt cacgctcggc
     6121 gcgcatacgg ttgtagatcg cttcagacac ttcggtcggc aggttgatct gcttaatacg
     6181 cacatcgaca acttcaatac ccagcgccgc catactgttc gggttgatga ccggaacttt
     6241 gcccttcgtc tcagccgtta cgcgctctgc cgcttcggca atggcgttat ctgctgccgg
     6301 ggtagtaact tcatcttctg tacccgcaga accggagttc agcgcgtcac gtacttcgag
     6361 ggtcagacga ccacgggaat cggtgacgat gtctttcacg tccaggcgac caatttcaga
     6421 acgcagacgg tcagagaact tacgtttcaa cagcacttcc gcttgcgaaa tgtcgccacc
     6481 acccgttgcc aggtagtaac ggctgaaatc gctgatgcgc catttgatgt aagagtcgac
     6541 gatcaggtct ttcttctctt tggtcacaaa gcggtcggcc tggttgtcca tggtctgaat
     6601 acgtgcgtcg agcattttca ccgtttcaat gaacggtatc ttgaaatgca gacccggctc
     6661 ataaaccaga ggtttgttgt catcgtcacg cagtacctta ccaaaacgca gcgtaatacc
     6721 gcgctcacct tctttgacga caaagacaga catgtaaagc actaccagca cgatgatgat
     6781 aatcgcgata actgacttac gcatcgttat tccccctgac gctggtagtc gttacgctgc
     6841 gcgttggcgc ggcgttggtc cataatatcg ccctgactgg tggacgacgt gttgcttgct
     6901 ccactggttg tggaggaaga ggctggcggc agacgcagca gattgctggc accgttatcg
     6961 ctcttcgccg caggggcgtt accacctttc agcatctggt ctaacggcag aaccatcaag
     7021 ttgccacctt tatcgttaac cagcactttg cgggtgttgc ccaacacttt ttccatcgtc
     7081 tcgatataca gacgctcgcg agtaatttcc ggcgcggctt tatattccgg cagaagttta
     7141 gcaaagcgcg ccacttcacc ctgagcttcc aggatggtct gggccttgta cgcacgcgcc
     7201 tcttcgagga tacgttgcgc ctgaccgttc gcacgcggct gaacttcgtt ggtatacgct
     7261 tctgcttcac gaatgtattg ctgttcgttt tcacgcgcgg caatcgcatc gtcaaacgcc
     7321 gcttttactt cttccggcgg acgagctgcc tggaagttga cgtccagcag cgtgataccc
     7381 atgtcatacg gacgaatcgt ctcttccagt tcgcgctgag tatcgctacg aatcacggta
     7441 cgaccttccg tcagaatgcg gtccatggtg tatttaccga taaccccacg cagggcgctg
     7501 tcggtagcct gacgcaggct gtcatccggg ctggtcacgc tatacagata tttttccgga
     7561 tcggtgacgc ggtactgcac gttcatctca acgcgcacta cgttctcgtc cgacgtcagc
     7621 atcacaccag aagcggccag ttcacgcacg gcttccacgt tcaccggttt gacttcgtcg
     7681 ataaacgtcg gtttccagtt cagacccggc tcaaccagat ggctgaattt accaaagcgt
     7741 gttaccacgc cacgttcggc ttctttaatg gtatagaaac cactggccgc ccagataatg
     7801 acaatcgctg ccgctgcgat ggtaacgaca cgaccgccaa gctgcgggcg cgggccttgc
     7861 gatgaactgc caccgccaga tccggtgcct ttaccgccgc ccagaccacc gagctttttg
     7921 ctcagtttgc ggaagatatc atctaaatca ggtggccctt gatcgcgacc gcctttgttt
     7981 ccatttccct cagagttgcc gccaggtttg ctgcttcccc acgggtcgcg gtcttgtccg
     8041 ttattaccgg gctgattcca cgccatgttt gtgctccata tttgttatgc ggtgatccct
     8101 gttgtctttc gacgccagag gatatgactc cacgcttcag acgctacgcc gttagatcag
     8161 gtaatcgatc aacgccggtt cttgtttaca gaggcgacgc cagtcaacga tcggcatacg
     8221 aacttgcaga cttacgctgc cgtcctcctc catccactct ttttctattg cctgaagctg
     8281 ataaaaacga cttctcagac gcccttcctg cggtggcaga cgcaatgtat gctgcgccac
     8341 ctcgccggaa agccgctccg tcaaagcctg aaaaagctgt ggtatcccct ctccggtctg
     8401 tgcggaaagc cagacacgga tcggtttgtt ctcttcgtcc cgatcaatac gcggttcgaa
     8461 atcttccagc atatcgatct tgttcatcac cagcagggtt gggatctcgt gagcgtcgat
     8521 ctcttcaaga accgtattca ccgcttcgat gttttcttgt acacgcacat ccgccgcatc
     8581 aatgacgtgc agcagcaatg tggcttgccg cgtctcttgt aacgtggctt taaatgccgc
     8641 caccagatcg tgcggcaggt ggcgaataaa ccctacggta tctgccaaca cggtttcacc
     8701 gacatctgcg acgtcaatac gccgcaacgt cgggtcgagg gtggcaaaca actggtctgc
     8761 cgcgtagacc cgcgcttcgg tgatgcgatt gaaaagggta gatttaccgg cgttggtata
     8821 tcccaccagc gaaacagtag gaacgtcggc tttgatacgc gattgccgcc cctgctcacg
     8881 ctgcttttca actctttcca ggcgcgactg tatctgcacg atgcgattac gcaacaaacg
     8941 acggtcggtt tcgagctggg tttcacccgg accacgcaaa cctatcccgc ctttctgtct
     9001 ttcaaggtgg gtccagccac gcaccaggcg cgtagccaga tggcgcagct gcgccagctc
     9061 aacctgcaac ttaccctcat gggtacgcgc acgttgggcg aaaatatcta aaataaggcc
     9121 ggtgcggtcg ataacacgac actcgcacaa acgctccagg ttacgctctt gcgccgggct
     9181 cagggcatgg tcaaaaagaa cgaccgaagc acccgtcgct ttgacagctt ccgcaatttc
     9241 aactgcttta ccttcaccta caaaatactt tgggtgcggc gctttacggc taccggtaat
     9301 cacctgcaat gcttcgacac cggcggaaga gaccagagat tcaaactcct ggaggtcttc
     9361 catatctttg tcttgcgtaa aatagatgtg taccagtacc gcctgctcac cagcatcata
     9421 acggtcaaac aagcgtataa ccctctaaat agatcagcgg ggaacgcagg atcgctggct
     9481 ccccgtgtaa aaaaaacagc ccgaaacctt attcggtttc ttcgctgtcc tgttgcgcgg
     9541 aagtattctg cgcgctgcta ccatgatggt agttactgct ggtaccgcca ccggcgttgt
     9601 tactgtgatg agaaaccggg cgagacggga caacagtaga aatcgcgtgc ttgtaaacca
     9661 tctggctgac cgtgtttttc aacaggatca cgaactgatc aaaagactcg atttgccctt
     9721 gcagcttaat accattcacc aaataaatag aaactggaac acgttcccga cgcagtgcgt
     9781 tcaggaacgg atcttgtaaa gattgcccct tagccattct ctcttttcct tatatgctta
     9841 tttgtacttt gaacctttcg attctgaaaa aattgcgcac gatacgtctc aattgtacac
     9901 attcagcctg cgatagcacc aacaacctgt aatacttcgt cacgcgcttg ttctggtttt
     9961 tcactgtcaa gccagtgaac cccttcccaa ccacgcagcc aggttatctg ccgcttcgcc
    10021 aactgtctcg tggcgcaaac acctcgataa accatttcat cgtatgagat ttcgccttca
    10081 aggtaagacc acatctggcg ataacccacg caacgaatgg aaggcaaatc cgtatgcaaa
    10141 tctcctcggg caaaaagcgc ccggacttct gcttcaaaac ctgaagccaa catctgatga
    10201 aaacgctgct caattcgttg atggagcagt tcacggctcg ccggggcgat ggcgaactga
    10261 tgcacctgat acggtagagc gtctcctgac gtttgcgtca gttccgttaa agttttaccc
    10321 gaaatgaaaa aaacttccag tgcccgggaa agcctttgtg gatcatttgg atgaatcctt
    10381 gctgccgcaa ccggatctac ctcctgaagt tgacgatgca atgactccca accttgctct
    10441 gccgcctgtt gctcaattct ggcccgcact tccgggtctg ccgacggtag cggcgacaac
    10501 ccttccagca atgccttgaa atacaacatc gtaccgccca ctaacagtgg gatccgcccc
    10561 gccgcggtga tatcggccat ttccgccagc gcatcgcggc gaaaatcagc agccgagtaa
    10621 gcctgcgacg gatcgcgaat atccagcaat cggtgcggcg cggcgagtaa ctcttcagcg
    10681 ttcggcttcg ccgtcccgat atccatccct ttgtaaataa gggcagaatc aacgcttatc
    10741 aactctactg gtaaaatttt acgcagctca atggctaacg ccgttttacc ggaggccgtc
    10801 ggccccatca aaaaaatcgc cttaggcagg ctcgccttac tgatatcact catctttcag
    10861 ggcttttatc gccggatgta aatcaacaga ttgtaacaga ccacccggcg gcgttttcac
    10921 aagttgcgga cataaccgtt ccacgtccgc cagcagggtt atggcctgcg ccattgacca
    10981 ctgcgcatgt tcgctcatca gatttcgtgc aatccactgc gcaatattgc caggttcgaa
    11041 tacggactgc ttcgccaggt agcctatcag ttcaggaatc aagatttgta aattttgttg
    11101 gcgtaagggt aaaggcacgg ccctgatggt cacatgctgc gcatcggact ggaaatcaat
    11161 acccaattcc gccagggcag actgcgcttt ttctaatgca gatttttctt cgccagaaac
    11221 ttttagccgc agcggaatca gcaacggctg ggcgcaaacg ggcgcttcac ccggcgtcaa
    11281 ttgtgcctga cgcagccaac gttctgccac tggcaaggct aaaagtgaaa tgttgccgtc
    11341 gcgctccagc aacgcacagt cggaatggac gatagtcagt acccgaccaa agctttgact
    11401 gttcgccgca agtgcaggtt cctgcggttc tggcgctttt ggtttttgca tcggcgcggg
    11461 cgtttgcaaa agctggcgat acacttcacc ttgctgtttc tggtaacctg gctgcgcatt
    11521 cggccagggg gcagccggac gactgcctga ggcgggcgca ggagtgtaaa gcggagctac
    11581 tggctcacga actgccggtt cagcaaaatg attgcgcccc gccgccacgc ggttttccgg
    11641 gatggcgcgc ggtgcaggtt gtggttcatc gtccagcggt agcggcgttt ccagttgctg
    11701 ttgtagcacg ctcagcacgc cctgatagat aaagtcatgc accagacgcg actgatggaa
    11761 acgcacttcg tgtttggcgg ggtgtacgtt gacgtccacc tgatgcgggt cgatctccag
    11821 atacagcaca aacgccggtt gttgatcggc ccccagtttg tcttcgcagg cctggcggat
    11881 cgcatgattg atcaggcgat cgcgcatcat gcgaccattc acgtagcaat actgaatttc
    11941 cgccagtgcg ggcgtggtgt gattcggatc tgccacccag ccgcgcagcg taagatcgcc
    12001 gtgttgccat tcaatcgcca gcgcttgttc gagaaaagcg gtgccgcaaa tcgcccctaa
    12061 acggcgctct ttttgtccac cttccggcac ggcgcggtac tggcggacca ttttcccgtt
    12121 atgcgacagg ttgatcgtga cgtcgaaacg cgccagcgca atgcggcgga tgatctcatc
    12181 aatgtggtta aattcggttt tctcggtgcg caggaatttg cgccgcgccg gggtgttgta
    12241 gaacagatcc agcacctcca gcgtagtccc cacaggatgc gccgccggtt ttaccgtcac
    12301 gtccatatcg cgcccttcgg cataggcctg ccaggcttcc tgctgttcta cggtgcgtga
    12361 agtgagcgtc aggcgggaaa ccgaactgat actcgccagc gcctcaccgc gaaagcccag
    12421 gctgataatg gcttcgagat cgtccagaga ggcgatttta ctggtggcat gacgagccag
    12481 cgccagcgcc agctcatctt ttttgatacc gcagccgtta tcacgaatgc ggataagttt
    12541 cgccccgcca cgttcgatat caatatcaat gcgcgtcgca cctgcatcga ggctgttttc
    12601 caccagttct ttgactaccg acgcaggtcg ctcgaccacc tcacctgcgg caatctggtt
    12661 cgccagttgt ggcggtaaga cctgtattgg catcaatcgt ccttagtttg gcagcgtacg
    12721 atctggcgtc gtcaccgtac tggcagtttg tgccgttgca ccctgcggcg cagattgcat
    12781 cggatgcgca aggaaataat tgcgcagacc tttataaatg gcttctgcca gctgttgttg
    12841 gtaatcgtcg ctcgccagca aacgttcttc gctgttgttg ctgataaaac cggtttcgac
    12901 cagtactgat gggatatccg gcgaacgcag aacgccaagg ctggcgtgtt ctggtcgacg
    12961 tttatgtatt tcgccaatgc gttgcaactg gctgatcata ctggtcgcta catcataccc
    13021 tacacgctgg gaatgaccga actgtaaatc cagcaccgcc tggcttaaat agggatcaga
    13081 ctgactgttc gccagcacat cgcccgctcc gcccagcagc tccgactgtt tctcgtgctg
    13141 ctccagccag ctggccattt cgctgttggc gcgacggtta gagagcaccc atacggaagc
    13201 gccagtcgca ctgcggtttg gtgcggcatc agcgtgaatc gacacgagga aattggcgtt
    13261 ttgcttacgt gccacatcgc tgcgccccat caccgagata aagtaatccc cgtcacgggt
    13321 taaaacgcct ttaaacattg gatcgtcatt gagaaaagta cgcaatttac gtgcgatggc
    13381 gatggtgaca tttttctccc gcgtaccacc ggggccgata gcgccagggt cctgaccgcc
    13441 gtgtcctgca tcaatagcga tgataatttt atcgccagtg ttagccgtcg cgcgcgctgc
    13501 cggacgcgtt accgtattac tgctgataac acctgtagtg cggttacttt ccgttttaaa
    13561 cggattgcgc gccggttcgc tgacgcgcgg tgcgacaacc gcaggcgttt caacgcgttt
    13621 agcaaccaca ggcggcgaag gaggcggtgg cggcacatcg gcgttaatcg taaagacgac
    13681 agtgtaattg ctgccattct gccgcttcac cgcttcggtt ttaccgtttt cggttagatc
    13741 gaccaccagc cgtagcgttt gtgcatcttt aggcgttccg gagcgaatcg ccttcaccag
    13801 attattgccg ctaaacaaca acggcagtcc ctgaatcacg cccgtttgtt tgatatcgag
    13861 cgccacggtg cgtttgcttt gatggctaaa cgcataatca ggatcgccaa taaaactcaa
    13921 cgttatccgc gcctgctgat tgccgttaga aacctgaata tcagagagcg tcgcggcacc
    13981 caccggcgcg cacagcagca gcaacgtcgc taccaaccaa tttctgatgc gatacatcat
    14041 cccgccacct ttcaaaggtt aaccggctaa acgcgccagc aacaattcac ccgcagagga
    14101 aaccgcactc acgcgcgcct cacggccttg tgcctgataa tcaatgtgta tttcgacatc
    14161 cgggtcagga agaacacctg taccttgttg tggccactcc accaggcaga tggcatcgtt
    14221 ggcaaaataa tcgcggatcc ccataaactc cagctcctcg ggatcggcaa ggcggtacaa
    14281 atcaaagtga taaaccatta aattgtcgag cgtatagggt tcgaccagcg tataagtggg
    14341 gcttttgaca ttaccctgat ggcccaaagc ctgcaaaaag ccccggctaa aggtggtttt
    14401 acctgcgcct aaatcgccat acagatagat tacggttgcg ccatcgcagg ctttcgctac
    14461 ccgctcgccc aggtctaatg ttgcctgctc atcagggagc ggaattactc gattcatcat
    14521 ggtttttatc agtcacttcc gggttaacaa tacgctgtag cgtggaaaag agatcggttg
    14581 ccagcatccc gcgcgttcca aaacgcgccg ccagtacgtc agctgccgca ccgtgcgcga
    14641 cacagcccgc acaggctgca tcatacggcg acagtttttg cccaagcaat gcgccaataa
    14701 taccagagag cacatcgccc atgccgccgc tcgccatgcc tgcattcccg gcatcaataa
    14761 tgcctaaagc gtcaggatgg gcggcgacca cggttccggc acctttcagc accgctacgc
    14821 cgccataacg ttgtaccaga cgtttggcgc aatgtaagcg gtcactttca atttcagcga
    14881 cggaacagcc taacaaccgt gcggcctcgc caggatgtgg cgtgatcacg cgattgtgac
    14941 gcttatcggg attgattgcc agcaggttca atgcatcggc atcccacaac atcggtttgc
    15001 gaaaattctc aactttttgc agggcttttt tcccccactc ttgctggccc agaccgggac
    15061 caatcaccac cacatcggcc cattccaggc tttcggtaag agagtccata gtcagttcat
    15121 gcaccatcaa ttctggtcgt gcagtcagca gcggcgcaat gttctcactg cgggtcagta
    15181 ctcggactaa accagcacca gcacgtagcg ccgcttcccc cgtcatacga atagcccccg
    15241 ccgtgccgtg atcgccaccg ataatcacca gccgcccgtg atcgccttta tgcgaagtcg
    15301 ggcgacgcgg tatcagccac tgagaaagtt gttctgccga aaaccgctga attttcgtct
    15361 cctgacctgc cagccagcta tccagcccca gtgagtcaaa atgcagttgt ccggtaacat
    15421 cccgcgcttt tccagtgagc aagcctggtt tcagcgcaat aaaagtgatg gtgtgatcgg
    15481 cgttgattac cgcgcctggc gtagcgccgg tttcagccag aaggccggaa gggatatcaa
    15541 ccgccacaat cggcgcaggg tgagtattag cgtggtcgat taactggcta atggattcgc
    15601 ggggcgcttg ctgcaagccg gtgccgagca gcgcatcaac aatcagatct accgattcgg
    15661 gccagacaat attcgaagca tggatctcgc ctcccgcgtt taaccatgct tcgcgtgcca
    15721 gcgcggcctc ttccggcaac ggtttgtcac tttcctgggc cagcagcgtg acatcaatac
    15781 cgatcgcttt ggccagccgc gcgaccacgt agccatcgcc gccgttatta ccatgaccgc
    15841 acagcaccag ccagtggcgg gcgtcaggat aagccgaacg acacacctgg aatgcggctt
    15901 cgccagcgcg aagcatcagc tcatagagtg tgagccccag cgcatctgcc gcctcgcgtt
    15961 ctccgcggcg gatatcgtcg gcgtaccaga cggtgtgtgg tatacttacg gggtttttct
    16021 tcattgtatg gtccgtcatg tcagagcccc tcgatctcaa tcaattagcg caaaatatta
    16081 aacagtgggg gctggaactg ggctttcagc aggtaggtat taccgatacc gatctcagcg
    16141 cgtccgagcc caaactgcaa gcatggctgg acaaacaata ccacggcgaa atggactgga
    16201 tggcgcgtca cggtatgctg cgtgctcgtc ctcacgaatt attgcccggt acgctgcgcg
    16261 tgatcagcgt tcggatgaat taccttcctg ctaacgccgc atttgccagc acgctgaaaa
    16321 atcccaaact cggctatgtt agccgttatg cgctgggccg tgactatcac aagcttctgc
    16381 gcaaccgact caaaaagctg ggcgagatga ttcagcaaca ttgtgtttcg ctgaatttta
    16441 gaccgtttgt cgattctgcg cctattctcg agcgcccgtt agctgaaaaa gcaggtcttg
    16501 gctggacagg taagcactca cttatcctca atcgcgaggc cggttcgttc ttctttttag
    16561 gcgaattgct ggtcgatatt ccgctgcccg tggatcaacc agtcgaggaa ggatgcggta
    16621 aatgcgtggc ctgtatgacg atttgcccga ccggtgccat cgtcgagcca tataccgtcg
    16681 atgctcgccg ctgtatctct tatctcacca tcgaactgga aggggcgatc ccggaagagt
    16741 tgcgaccgtt aatgggaaac cgtatttacg gttgcgatga ctgccagctt atctgcccgt
    16801 ggaatcgcta ttcgcaactc actacagaag acgatttcag cccgcgtaag ccgctacacg
    16861 caccggaact cattgagtta ttcgcctgga gtgaagagaa gtttttaaaa gtcacggaag
    16921 gttcggcgat tcgccgtata ggtcacctgc gttggctgcg taatatcgcc gtagcattag
    16981 gcaatgcccc ctgggatgaa acgattttga cagcgctgga aagtcgtaaa ggtgagcacc
    17041 cacttcttga tgagcacata gcgtgggcga ttgcgcagca aatcgagagg cgaaatgcgt
    17101 gcgtggtcga agtgcaatta cccaaaaaac agcgtctggt tcgggtgatt gaaaaagggt
    17161 taccgcgtga cgcctgattc atccacagct tgtgaataaa aataaaaacg tattgcaatt
    17221 caagacgaat aaaatcgtca agtgatcgac tcgacaaaac gaaatcttaa tttttaactt
    17281 atctatcaat aggttaaaaa aatactattc accgtgcgaa gtttctttga aattaagatt
    17341 acggcctgag gctgtggata actctgttta caagagtttg tcagagacaa caaaaaccag
    17401 caataacgca tcgcgtcgct gtggatattt tattgagaga agaa
//
LOCUS       DAZGQL010000101        17369 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.101, whole genome shotgun sequence.
ACCESSION   DAZGQL010000101 DAZGQL010000000
VERSION     DAZGQL010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17369
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.101"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            56..574
                     /locus_tag="ACHKIR_004022"
     CDS             56..574
                     /locus_tag="ACHKIR_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFO9991698.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNAQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            590..1129
                     /locus_tag="ACHKIR_004023"
     CDS             590..1129
                     /locus_tag="ACHKIR_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HFO9991699.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(1222..2799)
                     /gene="guaA"
                     /locus_tag="ACHKIR_004024"
     CDS             complement(1222..2799)
                     /gene="guaA"
                     /locus_tag="ACHKIR_004024"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFO9991700.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(2868..4334)
                     /gene="guaB"
                     /locus_tag="ACHKIR_004025"
     CDS             complement(2868..4334)
                     /gene="guaB"
                     /locus_tag="ACHKIR_004025"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFO9991701.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            4496..5872
                     /gene="xseA"
                     /locus_tag="ACHKIR_004026"
     CDS             4496..5872
                     /gene="xseA"
                     /locus_tag="ACHKIR_004026"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFO9991702.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDNAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQLKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANLRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPIVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     THQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPRIHRAQTRIQQLEYRLAETLRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTSAADGAVLKQVKQVKVGETLTTRLGDGVVISEVSAVTKTRK
                     SRKKTSNP"
     gene            complement(5941..13992)
                     /locus_tag="ACHKIR_004027"
     CDS             complement(5941..13992)
                     /locus_tag="ACHKIR_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF823 domain-containing adhesin"
                     /protein_id="HFO9991703.1"
                     /translation="MSLTLNKILVLCALLISAMLPGWSWAESAWQDSSDTVGEFNGTV
                     PTADSASIPVYQGSVFLDPAKTHEVAFTAKPSEFSADVSVSKLLVTNPQDREGDIIAT
                     PRWENQTPPTVSLVWADAATPDTLLDPQPVADRSFCAQGLAGRSLIAWAQPDPQQTMP
                     LLYLLTSTGYPYESVLTLADQKVTLKIAPAQGDLISVSAAGYDESSGAAKTTVGGSIT
                     LTVTTKDCVGNVVGNIPFVIKRKDAENRQGVVNNTAPVKLGTTELTTTATEYRGTTDA
                     NGVATVTVTQANGPGVKTPLVASLAGIAQTSETAVIFTVLTSPDVPQATMWGHMPDTL
                     KARDYTFSRPKLAAEVDNEDGTVNDHNETWSTFTWSGADKHCDILPGMRQFGALATVV
                     PTSVQDVAGWPMQGNYYWSSLAGMSGQHYAADVSNRSEAQKPDDTTFIVSCVDKEAPD
                     VEPKLVLTPGSYDSTIKAMKVKVGEEASLRLTITDSKNNDQPLAYYYFSLHLDDGVNR
                     KNQTDAAWETHPVQIDGGSNVRKVDAHTYEGITDANGEASLTLTQPGGVGVKTHITAK
                     MRSDYTASDEKDVIFTVITSPDSDKARMWGHMLGIIEANNIFKRPRLADETDNELGAV
                     RENNEDWALFDQNSSMQAECGLGHIPSQSSLHSLFAAHPANVIGTEYGWPTLQKAYLS
                     AVEETSHASVNLATGNIDTYSGFKQNYLSCSGNEMVAQIAATTDHDVSTSSRAQAKVG
                     ETITMTVRTFNALNNAPVPYTAFTITKDMGKNRQGQTTGFDDPTRGAIEMNGTLYGTS
                     QPSLVYAGTTDAQGFATVEIKQPQGVGLATPLNIAPVNSYIPNTVNYNVIFTTLTSPD
                     AVGAQMWGHMDETITVDALTFARPRLAAEVSSPDGTLTENNEVWSRVSQANTSSTSKG
                     GCGANMLPRRSQLNALYDANSGNAVQTVHGWPTQRQPYWSSSPADQVPHYYTIALNDG
                     ARTVGGSTAVYVSCLTTANNPASSITLEVVDPAQWNAAANAAKLKKGETLQVKVTVKD
                     AQGNPLGDMPFTLKRGDGYTRSNEKHIAGGGDALVAPVVINSGLADETSLNDTAAAYS
                     AMTGSDGTKILTITRPDTHGTKTSLTAALYSDTTKKATLDTIFTVVTSPDSDKAKMWG
                     HMPETVTAADGTVFKRPLLLKELSSTSGRTAIAEENEDWAQFTQTQAISTSSNGCGSE
                     YVPSQAGLESLYEANRGNAMKTVQGWPVASSYLSSTTGSSSLEQRDFKAVNLSSGTSS
                     IIPSATKELLTCQTTPIVKASQIVLEAADPTKFDSTNNVVKAKKGEEAVLRVTTKDAQ
                     GNPVGNTAFTLKRNTSVNRANVSTTTSIASLAVTDAWGNTQDDFLSTTLVIYGVTGAD
                     GITTFTLKQDQTTGLKTELTAALDSSSSTKSTLPVVFTVLTSPDSPKAKFWGHMAETA
                     TGDDGLIYRRPLLRDENSATTSIGTLVEEGEAWSTFPSGQANDTSINGCGAEYVPTDN
                     ELRAIYAHQGSSALHDAIGWPVSRFYISNTVADTFTQTFTYDVVSLKTGDETQMPSSG
                     GALLSCRTTPVAVASQIIVEANDTAQFVKVDDTLSALKVKKGEDAVIRVVTKNAQGNS
                     VPNVPFILRREGSKNRQNAEMINKSITVINAAGASARMNSSSSLLYGVTGADGTTSFT
                     VKQDDSMGLVTNMYAQLYQPTIESNKLPVMFTVITSPDTPLASYWGHMPETFTTRSGI
                     AFKRPLLTAEHPAGQSTMANNESWLSLNTAAKNDVSKSDCGEPYQPLLSEFQELYSEH
                     PNGAIGTDLGLPLTNTWWAYDKIAYANVWYDQSINLSNGSSSRALSNTVAFVSCLVNP
                     HAVAASIEMTSTALDAEKTASNDGRPSATAAKGTAIRMTVIVRDSAGNLLPGANFNLI
                     RGTALDRAKNRLDSTYDDLTIVPVTPAGVNMSLYNNGAQALLTTGSDGKATFDVTQNE
                     TYGLATPLTATLMRDTTKSATMDVIFTVITSPNSPKAKYWGHMPDTFTSRAGVTFKRP
                     LLAAEATLGSSVSNNNESWSYLFYTNKVTPDCPVEYQPRLNELQGLYNDHPGGTILTD
                     LGLPITAGSGNWWTYEMSTTDALTWYYGVINLKTGQSTTTINGYALMLCLTQPHSAPA
                     SLTLSSTAYDEGRTASNGGTPTSSVKKGEMLPIVVTIKDANGNPVGGEGVTLKRVQAK
                     SRSGISVSSNTVDDLILDEVTPTSARISFNQNTSAWSGFTGSDGTITFNVTQNNTVGL
                     VTPFTASLARNPQVTANQDLIFTVVTSPDSAKANYWGHMPATLTAVNGAVFERPKLWS
                     ELTSTSGVGKINNNNEDWPYFTPTQKSDASVSPCEVARQPLFNDLSSLSARYPNNTFV
                     TETGWPAYYTWWAEDKSADGKDQSVDLRNGTLYTGSTKSFQPCLANARSTVSSVTLTS
                     TAFDAATQAAKVKKGEAMPVTVTVKDSAGNTVPNVEFTLKRGEASPRNAGATLYGDVV
                     AMDDLIVQPLSGSAVTLSESGNTISGMTGADGTASFTLRQDNTPGYKTPLTVTLANYA
                     SATDTLDAIFTVPTSPNVSSAHFWGHMADTVVVNGKSLHRPLLTTELPSGANPVSSPI
                     INYENWASAHIIDASKWDIARQCGSIENAPTYNELELLHTVFNSLGWPSSPSFPYLSS
                     QQCGMDEGTGAQDCSITLINKPGLVTCFQ"
     gene            complement(14113..15090)
                     /locus_tag="ACHKIR_004028"
     CDS             complement(14113..15090)
                     /locus_tag="ACHKIR_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SinI family autotransporter-associated protein"
                     /protein_id="HFO9991704.1"
                     /translation="MKQDKRRGLTRIALALALAGYCVAPVALAEESAWVDSGETNIFQ
                     GTIPWLYSEGGSATTDADHVTLTSDLKGARPTGSETDKRLYSGDKLTVSWEIGDTEGD
                     VDLGGLGDNAKTIDTIRWMSYKDAQGGDPKELATKVTSYTLTDDDRGRYIGIEITPTT
                     QTGTPNVGTALHLYDISTASGGGSDSDNVAPGPVVNQNLKVAIFVDGTSINLINGSTP
                     IELGKTYVAKLYSDENKNGKFDAGTDADVTANYDFRWVLSGSSQQLGTSGGIVNSSFD
                     NNNLAIPATNDEARTNLNGPARDGKEALTIPTNGDGVQGYKLHIIYKHK"
     gene            complement(15145..17322)
                     /gene="sinH"
                     /locus_tag="ACHKIR_004029"
     CDS             complement(15145..17322)
                     /gene="sinH"
                     /locus_tag="ACHKIR_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001317981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intimin-like inverse autotransporter SinH"
                     /protein_id="HFO9991705.1"
                     /translation="MLRWKRCIILTFISGAAFAAPEINVKQNESLPDLGSQAAQQDEQ
                     TNKGKSLKERGADYIINSATQGFENLTPEALESQARSYLQSQITSTAQSYIEDTLSPY
                     GKVRSNLSIGQGGDLDGSSIDYFVPWYDNQTTVYFSQFSAQRKEDRTIGNIGLGVRYN
                     FDEWLLGGNIFYDYDFTRGHRRLGLGGEAWTDYLKFSGNYYHPLSDWKDSEDFDFYEE
                     RPARGWDIRAEAWLPSYPQLGGKVVFEQYYGDEVALFGTDNLEKDPYAVTLGLNYQPV
                     PLLTVGADYKAGTGDNSDVSINATLNYQFGVPLKDQLDSDKVKAAHSLMGSRHDFVER
                     NNFIVLEYKEKDPLDVTLWLKADATNEHPECVIKDTPEAAVGLEKCKWTINALINHHY
                     KIVAASWQAKNNAARTLVMPVIKENTLTEGNNNHWNLVLPAWQYSSDKAEQEKLNTWR
                     VRLALEDEKGNRQNSGVVEITVQQDRKIELIVNNIANVPEENNHSHEASAQADGVDGV
                     VMDLDVTDSFGDNTDRNGDALPEDNLTPRLYDAQDKRVTLTNKPCSTDNPCVFIAKQD
                     KEKGTVTLSSTLPGTYRWKAKAAPYDDSNYVDVTFLGAEIGGLNAFIYRVGAAKPSNL
                     IGKDKEPLPLNNTYRFVLWRDSNKDGVFQQVEKLTDEEMAQYDYKWEFTGKSINGEVG
                     AQANTSNEDIVIPATNREAAQTYGAQAGDGLQGYGLRVLYTKK"
BASE COUNT         4084 a         4615 c         4309 g         4361 t
ORIGIN      
        1 cctcttttga tgatcttaaa tgtcttattt ttcgtaatgt gtataacaag gaatagtgat
       61 gaaatttaaa aaatgtcttc tgcctgtggc aatgttagcg tcattcactc tggcaggatg
      121 ccagtcaaat gctgatgatc atgccgccga tgtttatcaa accgatcaac tgaataccaa
      181 acaagaaact aaaaccgtta atattatttc cattcttccc gcaaaagttg ccgtagacaa
      241 cgcccaaaat aaacggaacg cacaagcctt cggcgcgctt attggcgctg tcgctggcgg
      301 tgttatcggc cacaacgtcg ggtctggcag caattccgga acgacggcag gtgcagttgg
      361 cggcggagct gtaggcgcgg cagcgggttc tatggtgaat gataaaacct tagtggaagg
      421 tgtttcttta acatataagg aaggcaccaa agtgtatacc tccacccagg tgggtaaaga
      481 gtgccagttt acgacaggtt tagccgttgt tattaccacg acgtataacg aaacacgtat
      541 tcagccaaat accaaatgtc ctgaaaagag ctaataatca ggaggagtca tgaagaaagt
      601 ttttctttgc gccatcttag cctccttaag ctatccggct atcgcctcat cattgcagga
      661 tcaactctcg gctgtcgcag aagcggaaca gcaaggtaaa aatgaagagc aaaggcagca
      721 tgacgaatgg gtcgcggagc gcaacaggga aatccagcaa gagaagcaac gtcgcgcaaa
      781 cgcccaggcc gcggctaata aaagagcggc aacggcagcg gcgaataaga aagctcgtca
      841 ggataaactg gacgccgaag ccactgcgga caaaaaacgc gatcaaagtt atgaagatga
      901 gctacgcagc ttagagattc agaaacaaaa actggcgctg gcgaaagaag aagcccgcgt
      961 taagcgagaa aacgaattta tcgatcagga actgaagcac aaagctgcgc aaaccgatgt
     1021 ggtgcaatct gaagctgacg caaacaggaa tatgactgaa ggcggtcgcg atctgatgaa
     1081 aagcgtgggc aaagcagaag agaataaatc agatagctgg tttaactaaa cgatgttagt
     1141 aacttcaaac ctataattct ttaggataaa aaaccctctg tagtaacaga gggttttgtt
     1201 cattcatagt gcagggtcaa atcattccca ctcaatagtt gctggtggct tgccgctgat
     1261 gtcatacacc acgcgggaaa taccattcac ttcattgata atgcggttgg aaacgcgacc
     1321 gaggaaatcg tacggcagat gtgcccagtg tgcggtcata aagtcgatgg tttcgacagc
     1381 acgcagagag acaacccagt catacttacg accatcgccc attacgccaa cggaacgtac
     1441 tggcaggaag acggtgaatg cctggctgac tttgtcgtac aggtccgctt tacgcagttc
     1501 ttcaatgaag atggcgtcag cacggcgcag caggtcacaa tactctttct tcacttctcc
     1561 cagaacacga acgccaaggc ctggtcccgg gaacgggtga cggtacaaca tgtcatacgg
     1621 taggcccagc tccagaccaa tcttacgcac ttcgtctttg aacagctctt tcagcggttc
     1681 aaccaagccc atcttcatct ctttcggcag gccacccacg ttgtggtgag atttgatgac
     1741 gtgtgcttta ccggttgcag aagcagcaga ttcgataacg tcagggtaga tagtgccctg
     1801 cgccagccac ttcacgtctt ccagtttcag cgcttcttca tcgaatactt caacgaaaac
     1861 gcgcccgatg attttacgtt ttgcttccgg atcattttcg ccagccagcg ctgacaagaa
     1921 gcgatcttct gccggtacgt gaacaatatt cagaccaaag tgatcgccaa acatatccag
     1981 aacctgctct gcttcgttga gacgcagcag gccgttgtcg acgaatacgc aagtcaggtt
     2041 tttaccgata gcgcggtgca acagcattgc ggttacggag gaatccacac caccagagag
     2101 gccgaggatg actttatcgt cgcctacctg ctcgcggatg cgagctacag catcgtcgat
     2161 aattttcgct ggcgtccaca gggcttcaca ctggcagata tcacgcacaa aacgctccag
     2221 catgcgcata ccctggcggg tgtgagtcac ttccgggtgg aactgtacgc catagaagcg
     2281 tttttcttcg ttagccataa tggcaaacgg gcagctttcg gtgctggcta cggtgacgaa
     2341 gtcggacggg atagcggtaa ctttatcgcc gtggctcatc cagacatcga gcagcggttt
     2401 accgtctgcg gtcagagcat cttcaatacc gcgaaccagt gcgctgtcgt ttacgacttc
     2461 aacctgcgcg tagccaaatt cacgttcgtt agaggcttca acgtgaccgc ccaactgcat
     2521 tgccatggtc tgcatgccat agcaaacgcc gaataccggt acgcctgctt caaaaacata
     2581 ctgcggcgca cgcgggctgt tttcttcggt ggtgctttcc gggccgccgg aaagaataat
     2641 gccgcttggg ttgaagtcac gaatttgtgc ttctgtcaca tcccacgccc acagttcgca
     2701 gtaaacaccc agttcacgca cgcggcgcgc aaccagttga gtgtactggg aaccgaagtc
     2761 cagaatgagg atgcgatgct tatgaatgtt ttccgtcatt gacgcttatt ccgaggcaag
     2821 tgaaacagat aatataaatc gcccgacatg aagtcgggcg aagagaatca ggagcccaga
     2881 cggtagttcg gggactcttt agtaatggtc acgtcgtgaa cgtggctttc ctgaatgccc
     2941 gcaccgctga tacgtacaaa ctccgcttta gtacgcagtt cgtcgatagt accacagccg
     3001 gtcagcccca tacaggagcg caggccgccc atctgctggt gaatgatctc tttcaggcga
     3061 cctttatagg ccacgcgacc ttcgatacct tccggcacca gtttgtcggc agcgttgtcg
     3121 ctctggaagt aacggtcaga agaacctttg gacatcgcgc ccagggaacc cataccacgg
     3181 taagatttgt aagaacggcc ctggtagagt tcgatttcac ccggagattc ttcagtacct
     3241 gccagcatgg aacccaccat caccgcgctt gcgcctgcgg cgatagcttt ggcgatatcg
     3301 cctgagaaac ggataccgcc gtcagcgata accggaatgc cagtaccttc cagggcttct
     3361 actgcatcag caacggcggt gatctgcgga acaccgacac cagtcacgat acgcgtcgta
     3421 cagatagaac cagggccgat acccacttta acggcactgc aaccggcttc tgccagcgcg
     3481 cgggcacctg cagctgttgc cacgttgccg ccgatgattt gcaggtcagg atatttagcg
     3541 cgagtttcac ggatacgctg cagaacacct tcagagtgac cgtgagagga gtcgatcagc
     3601 agaacgtcaa cgcctgcggc aaccagcgca tcaacacgct cttcgttacc agcgccagca
     3661 ccaaccgctg caccaacacg cagacggcct tgctcgtctt tacaggcgtt cggtttacgt
     3721 tctgctttct ggaaatcttt cacggtgatc atgccgatca ggtggaattc gtcatcaacc
     3781 accagcgctt tttcaacgcg tttttcgtgc atttttgcca gcaccacttc acgggcttcg
     3841 ccttcacgca cggttaccag acgctctttt ggcgtcatgt aaacgctaac cggttgattc
     3901 agatcggtca caaaacgcac gtcacgaccg gtgatgatgc cgaccagttc gttttcttcg
     3961 gtaacgaccg ggtagcccgc aaagccgtta cgctcggtca gttctttcac ttcgcgcagc
     4021 gtcgtggttg gcagaacagt ctgcgggtca gtcaccacac cagattcgtg ttttttcaca
     4081 cggcgaactt cttctgcctg gcgttcaatg gacatgtttt tgtggataaa gccgataccg
     4141 ccttcctgag ccagagcaat agccaggcgc gcttccgtta cggtatccat tgctgcggaa
     4201 agcataggga tattcagacg aatagttttc gtcagctggg tgctgaggtc agcagtattc
     4261 ggcagaacgg tagaatgtgc aggaacgagg agaacgtcgt caaacgtcag agcttcttta
     4321 gcgatacgta gcatgggcaa tatctcgacc agagtggtta ataaatattg ccgcggcatt
     4381 atacagagcg taaccgattg catctacccc tttttgcaaa aaatgcttgc tatccccgga
     4441 gggcgggtta ctatcgactg aataacctgc tgatttagaa tttgatctcg ctcacatgtt
     4501 accttctcaa tcccctgcaa tttttaccgt tagccgcctg aatcaaacgg ttcgtctgct
     4561 gcttgagcat gagatggggc aggtgtggat cagcggcgaa atctctaatt tcacacaacc
     4621 ggcttccggt cactggtact ttacgcttaa agacgataac gcccaggtgc gctgcgcgat
     4681 gttccgcaac agcaatcgcc gggtcacctt ccgcccgcag catgggcaac aggttttagt
     4741 tcgcgccaac attacgctgt atgaaccgcg cggcgattat caaattattg tcgaaagtat
     4801 gcagcccgcc ggtgaaggtt tactgcaaca gaagtacgaa cagcttaaag cgaagttgca
     4861 ggctgaaggt ttgttcgatc agcaacttaa aaaaccactc ccctcccctg cgcactgcgt
     4921 tggggtgatt acctcaaaaa caggtgccgc actgcacgat attttgcatg tgttgaaacg
     4981 ccgcgatccg tctctgccag tgataatcta tcccactgcc gttcagggcg atgacgcacc
     5041 agggcaaatc gttcgcgcca ttgagctggc gaatcttcgc aacgagtgcg acgtattaat
     5101 cgtcgggcgt ggcggtggct cgctggaaga tttatggagt tttaatgatg agcgtgtggc
     5161 gcgggcgatt tttgccagtc gcattccgat agtcagcgcc gtcgggcatg aaaccgatgt
     5221 gacaattgct gactttgttg ctgatctacg tgcgccaacg ccgtcagccg ccgctgaagt
     5281 ggtgagccga aatcagcaag aattgctacg tcaggtgcaa tcgacccatc aacggctgga
     5341 aatggcgatg gattattatc tcgccaaccg cacgcgtcgt tttacgcaga ttcatcatcg
     5401 cttgcagcaa cagcatccgc agctccggct ggcacgtcag caaactatgc tcgaacgtct
     5461 gcaaaagcgg atgagctttg cgctggaaaa tcagcttaag cgtgccgggc aacagcagca
     5521 gcgattaaca cagaggctaa accagcaaaa tccgcagccg cgtattcacc gcgcacagac
     5581 gcgaattcaa caactggaat atcgtttagc agaaaccctg cgcgcacagc ttagcgccac
     5641 gcgtgaacgt ttcggtaatg cagtaacaca cctcgaagcc gtaagcccac tgtcaacgct
     5701 ggcgcgcggt tatagcgtca ccagcgccgc tgatggcgcg gtgttaaaac aggttaagca
     5761 agtgaaagtg ggtgagacac tgaccacacg actgggagat ggtgtggtga ttagtgaagt
     5821 cagcgccgta acaaaaaccc gcaaatcacg taaaaaaacg tctaatccgt agaccggata
     5881 agaggcgcca gcatcgcatc cggcaataac tcccaggcca cgccagatcc gccatgctgg
     5941 ttactggaag caggtcacca gacccggttt atttattagc gtgatactac aatcctgtgc
     6001 tccggtacct tcgtccatcc cacattgttg agaggacaaa taaggaaaac tgggtgaaga
     6061 cggccatccc agtgaattaa aaacagtatg caacaactct aattcattgt atgtcggtgc
     6121 attttctatg ctgccacact gccttgcgat atcccattta ctggcatcaa tgatatgtgc
     6181 agaagcccag ttttcgtagt taataatcgg tgaggatacg ggattagccc cggacggcaa
     6241 ttctgtcgtt aacaacggtc gatgtagcga tttaccattc accactaccg tgtctgccat
     6301 atgtccccaa aagtgcgccg aggaaacatt tgggctggtt ggtaccgtaa aaatagcgtc
     6361 cagcgtgtct gtcgcggaag cgtaattagc cagagtaacc gtcagtggcg ttttataacc
     6421 aggggtatta tcctggcgga gcgtaaaact tgccgtccca tccgcgcctg tcatgccact
     6481 aatggtatta ccgctttcac tcagcgtaac agccgaacca gataacggct gaacgatgag
     6541 atcgtccatc gccaccacat caccatacaa cgttgcccca gcgttacgcg gcgatgcctc
     6601 accgcgtttc aaagtaaatt ccacattcgg caccgtattc ccggcgctat ctttcaccgt
     6661 cacggtaacg ggcatggctt cgcctttttt cactttcgcc gcctgagtgg ctgcatcaaa
     6721 cgctgttgag gtaagtgtga cgctggaaac cgttgaacgc gcgttagcca ggcaaggctg
     6781 aaatgatttt gttgaacccg tatacagcgt gccatttctc aaatcaaccg actggtcttt
     6841 accatcggcg gatttatcct ctgcccacca agtgtaatac gcaggccagc cagtttcagt
     6901 gacaaacgtg ttattcggat aacgcgcaga caacgaactt aaatcattga atagtggctg
     6961 ccttgctact tcacaagggc tcacactcgc atctgatttc tgtgttgggg taaagtacgg
     7021 ccagtcttca ttattgttat tgatcttgcc gacgccagaa gtcgatgtca gttcactcca
     7081 caatttcggg cgttcaaata ccgcaccatt cactgccgtc aaggttgctg gcatatgccc
     7141 ccagtagtta gccttcgcag aatccgggct ggtcactacc gtgaatatca ggtcctgatt
     7201 agccgtaacc tgcggatttc gcgccaatga agctgtaaac ggcgttacca gaccgacggt
     7261 gttattttgg gtaacgttga acgttatcgt gccatcactg ccggtgaagc cgctccatgc
     7321 cgacgtattc tggttgaagg agatacgcgc cgatgtcggg gtgacttcat ctaaaatgag
     7381 atcatctacg gtattggagg atacagagat ccctgaacgg cttttcgcct gcacgcgctt
     7441 gagcgtaaca ccttcgccgc caacaggatt accattggca tctttgatcg tcaccacgat
     7501 gggaagcatc tcaccttttt tcacagatga cgtgggggta ccaccattgc tggcagttcg
     7561 gccctcatca taggcagtcg atgacagcgt aaggcttgct ggtgccgaat gtggctgcgt
     7621 cagacagagc attaaagcgt atccattaat agtcgtcgtc gattgtcccg ttttcaaatt
     7681 aatgacgccg taataccacg tcaacgcgtc agtggtgctc atttcataag tccaccaatt
     7741 tccacttccg gcagttatag gcaaccccaa atctgtcagt attgtcccgc ctggatgatc
     7801 attatatagc ccctgtaact cattgagacg cggctgatac tcaaccgggc aatccggcgt
     7861 aactttattg gtataaaaaa ggtatgacca cgactcatta ttattactta ccgaggaccc
     7921 caaggtcgct tctgctgcca gtagcggtcg tttaaaggtc acccccgccc tactggtaaa
     7981 cgtgtcgggc atatgacccc agtattttgc tttcggtgag ttcggactgg ttatcacagt
     8041 gaaaataaca tccatcgtgg cactttttgt ggtgtcgcgc atcaatgttg cagtgagcgg
     8101 agttgccagc ccataagtct cgttttgcgt cacatcaaaa gtcgctttac catcacttcc
     8161 tgtcgtcaga agtgcctgtg ccccattatt atagagtgac atgtttactc cggcaggggt
     8221 gaccggcacg atagtaagat catcatacgt agaatctagc ctgttttttg cacgatccaa
     8281 cgcagtcccg cgtatgagat taaagtttgc gcctggcaac agatttcctg cgctatcccg
     8341 cacaatcacc gtcatccgga tcgccgtacc tttcgccgcc gttgcactag ggcgaccatc
     8401 gttgctggcc gttttctccg catccagtgc cgtggaggtc atctctatag aagctgccac
     8461 tgcatgaggg tttaccagac aggacacaaa tgccacggtg tttgataatg ccctggagct
     8521 tgatccattc gataaattaa tagactgatc gtaccagacg ttcgcgtagg caattttatc
     8581 gtaagcccac caggtgttag tcagcggaag gcctaaatcg gtccctatcg caccattagg
     8641 atgttcactg tataactctt gaaattcgct taataacggt tgataaggtt cgccgcagtc
     8701 tgatttgctg acatcatttt ttgctgccgt attcaaagaa agccatgatt cattattagc
     8761 catggttgac tgccccgctg gatgctcggc agtaagcaat ggccgcttaa aggcaattcc
     8821 cgaccgggta gtaaacgttt caggcatatg cccccagtaa cttgccagtg gcgtatccgg
     8881 gctggttatg acggtaaaca tgaccggtaa tttgttggat tctatcgtcg gctgatacaa
     8941 ctgggcgtac atattggtta ccaggcccat agaatcatct tgttttaccg taaaggaggt
     9001 agtcccatcc gcgccagtca ccccataaag caggcttgaa gaactattca tcctcgcaga
     9061 agcgcctgcc gcgttaatta ccgtaatcga cttatttatc atctcggcat tttgacggtt
     9121 cttcgagcct tcacgtctga gaataaaggg gacattgggc acgctatttc cctgcgcatt
     9181 tttagtcacc acgcgaatca ccgcgtcttc gccttttttc actttcaacg cactcaaggt
     9241 gtcgtcaact ttgacaaatt gcgccgtatc attcgcctca acgatgatct gactggcaac
     9301 ggcgacaggc gtggtccggc agctcagtaa tgcaccacca gatgagggca tctgcgtttc
     9361 atctcctgtc ttcagcgaaa cgacatcata cgtaaaggtt tgagtgaacg tatctgcaac
     9421 ggtattcgag atatagaacc tcgacactgg ccagccaata gcatcatgta atgcgctact
     9481 cccctggtgc gcataaatgg ccctaagttc attgtcagtt ggaacatact ccgcgccaca
     9541 accatttatc gaggtatcgt tagcctggcc cgacgggaac gtagaccagg cctccccctc
     9601 ttccaccagg gtaccgatac tggtggtagc actattctcg tccctcagta aaggccttcg
     9661 ataaataaga ccgtcatcgc ctgtcgccgt ttcagccata tgcccccaga atttggcttt
     9721 tggcgaatcc gggctggtaa gcaccgtaaa gacaacggga agcgtcgatt tagtgctgct
     9781 ggaggaatcc aacgctgccg tgagttctgt ttttagcccg gtagtctggt cttgcttcaa
     9841 tgtgaaagtg gtgatgccat cagcccccgt cacgccataa ataaccaggg tcgttgaaag
     9901 aaaatcatct tgagtatttc cccaggcatc cgttaccgcc agcgaagcaa tcgacgtcgt
     9961 agttgacaca ttggccctgt tcacgctggt gtttcgtttg agggtaaatg cggtatttcc
    10021 cacagggtta ccctgcgcat ctttagtggt cactcgtaat acggcttctt cgcctttttt
    10081 cgctttgaca acattattcg tactgtcaaa ttttgtgggg tctgcagctt caagaacaat
    10141 ttggcttgcc ttaacaatcg gcgtcgtctg gcaggtaagc agttcttttg tggcagatgg
    10201 aattatcgaa ctggtgcctg aactgagatt gacggccttg aagtcacgct gttccaggct
    10261 actactacct gtcgtgctgg aaaggtaact tgaggcgacg ggccagccct gaaccgtttt
    10321 catcgcattg ccgcggttgg cttcatacaa tgactccagc ccggcctggg agggaacata
    10381 ttcactacca caaccattag aggatgtgct tatagcctgg gtttgggtaa attgcgccca
    10441 gtcttcattc tcttctgcaa tcgctgttcg acccgacgta ctggataact ctttcaacaa
    10501 taatgggcgt ttaaagaccg ttccatctgc agccgtcact gtttcaggca tatgccccca
    10561 cattttcgcc ttatcgctgt ccgggctggt gacaacggta aaaatggtat cgagggtcgc
    10621 ttttttcgtt gtgtctgaat agagcgcggc ggtcagcgac gtttttgtgc catgggtatc
    10681 cgggcgggta atagtgagaa ttttagtgcc atcgctgccg gtcatcgcgc tgtatgctgc
    10741 ggcggtatca ttgagcgaag tttcatccgc caggccacta ttgatcacaa ccggcgcaac
    10801 cagcgcatca ccgccaccgg caatgtgttt ttcgtttgat cgggtataac catcgccacg
    10861 cttcagcgta aaaggcatat cacccaatgg attgccctga gcatctttta ccgtcacctt
    10921 cacctgtagc gtttcgcctt ttttcagctt cgccgcattt gctgccgcgt tccactgcgc
    10981 cgggtccacc acttccagcg taatgctgct ggcaggattg ttagccgtcg tcaggcaact
    11041 gacataaacc gccgtgctac cgccaacggt ccgcgcgcca tcgttgaggg ctatggtgta
    11101 gtaatgcggc acctgatccg cgggcgaact gctccagtag ggctgacgct gcgttggcca
    11161 accgtgtacc gtttgaacgg cgttaccgct gttagcatca tagagcgcgt taagctggga
    11221 acggcgtggg agcatattgg caccacagcc acctttactg gtgctggaag tgttggcctg
    11281 gctaacgcgc gaccacactt cgttattttc cgttaatgtg ccgtccgggc tggacacttc
    11341 cgcagccagc cggggtcggg caaaggttaa tgcatcgacg gtaatcgtct catccatatg
    11401 accccacatc tgcgcgccaa ccgcatccgg actggtgagc gtagtgaaaa tcacattgta
    11461 attaacagta ttgggaatat aggagtttac aggcgcaata ttcagcggcg tcgctaagcc
    11521 cacaccttgc ggttgcttaa tttccacagt agcaaaaccc tgcgcatccg tagtaccggc
    11581 atagactaaa gacggttgcg atgttccgta gagtgtgcca ttcatctcga ttgcgccgcg
    11641 ggttggatca tcaaaacctg tagtctgccc ctgacgattt ttccccatat ctttagtgat
    11701 ggtgaacgcg gtatacggca cgggcgcatt attcaaggca ttaaaggtgc gtaccgtcat
    11761 ggtaatcgtt tcacccactt tagcctgcgc tctgctgctt gtggaaacat catgatcggt
    11821 ggtcgcagca atctgggcga ccatctcatt gcccgaacaa gaaaggtagt tttgcttaaa
    11881 gccggaatag gtgtcgatgt tgcccgtggc caggttcacc gacgcgtgcg acgtctcttc
    11941 caccgcacta agatacgctt tttgcaacgt tggccagcca tattcggtgc cgatgacatt
    12001 ggctggatgt gcggcaaaca aactgtgcaa tgagctttgg ctcggaatat gacctaaacc
    12061 acactcagcc tgcatactgc tattctgatc aaacagcgcc cagtcttcgt tattttcccg
    12121 cactgctccc agttcgttgt cggtttcatc cgccagccgt gggcgtttaa agatattatt
    12181 cgcttcaata atgcctaaca tatgtcccca catccgcgct ttgtcggaat cagggctggt
    12241 gatcaccgta aaaataacgt ctttctcatc cgaggcggta taatcactgc gcattttcgc
    12301 ggtgatgtgt gttttcaccc ccacgccgcc tggctgggtc agcgtcagag aggcctctcc
    12361 gttagcatca gtgatccctt cataggtatg cgcgtcaact ttgcgaacgt tactgccacc
    12421 atcaatttgt accgggtgcg tctcccacgc agcgtcagtt tgattcttac ggttaacacc
    12481 gtcatccaga tgcagcgaaa agtagtagta agccagcggc tgatcgttgt tcttgctatc
    12541 ggtaatcgtc aggcgtaaag aagcctcttc ccccaccttt actttcatcg cttttatcgt
    12601 cgaatcataa ctgcccggcg tcagtaccag cttcggttca acatcgggcg cttctttatc
    12661 gacacagctc acaataaacg tcgtatcgtc cggtttttgc gcttcgctgc ggttcgaaac
    12721 atccgccgca tagtgctgcc cgctcatccc cgccagtgat gaccagtagt agttaccctg
    12781 catcggccaa ccagccacat cctgcactga cgttggcacc accgtcgcca gcgcaccgaa
    12841 ctgacgcatt ccgggcaaaa tgtcgcaatg tttatccgca ccactccagg taaaggtgga
    12901 ccaggtttca ttgtgatcgt taaccgtacc gtcttcatta tcgacttccg ccgccagttt
    12961 tggtcgacta aaagtgtaat cacgggcctt cagcgtatcg ggcatatgac cccacattgt
    13021 ggcctgcggg acatccgggc tggtgaggac ggtaaagatc actgccgttt cactggtctg
    13081 agcaataccc gcgaggctcg cgaccaacgg cgtttttaca ccagggccgt tggcctgggt
    13141 gaccgtaacc gtggcaacac cattcgcatc ggtagtacca cgatattccg tcgcggtggt
    13201 ggttaattcg gtcgtgccca gttttaccgg cgcagtattg ttcactacac cctggcggtt
    13261 ttcagcatct ttgcgtttga taacaaaagg aatattcccc acaacattac cgacgcaatc
    13321 tttggtggtg accgttaacg tgatactgcc gccaaccgtc gttttcgccg caccggagga
    13381 ttcgtcatag cccgcggcgc tgacggaaat aaggtcaccc tgcgcgggcg caatcttcag
    13441 cgtcactttc tgatcggcaa gggtcagaac actttcatag ggatagcccg tcgaggtaag
    13501 tagatataac agcggcatgg tctgctgggg gtctggctgc gcccaggcga tcagcgaacg
    13561 cccggcaagg ccctgtgcgc aaaatgagcg atctgcgact ggctgtgggt ccagtaaggt
    13621 gtccggcgtc gcggcatccg cccaaactaa agatactgtc ggtggcgttt ggttttccca
    13681 acgtggtgtg gcaatgatat cgccttctct gtcttgcggg ttggtgacga gcaatttaga
    13741 gacgctaaca tccgcgctga attcactcgg tttagcggta aacgctacct catgcgtctt
    13801 tgcaggatcc agaaagacac tgccctggta gacgggtatg ctggcactgt cggcggtggg
    13861 cacggtaccg ttaaattcgc cgaccgtatc tgaagagtct tgccatgcag attctgccca
    13921 tgaccaccca ggcagcattg cgcttatcag caatgcgcat aacacaagga ttttattcag
    13981 ggtcagggac atgacagaaa ctccaccggt tgttaaacgg acgcttgtcg catcaacata
    14041 ttttgcttaa tccgggatta agcaaaatat gcggaaacag cattcccttg cgggaatgct
    14101 gctgagtacg cgttatttgt gtttgtaaat aatgtgaagt ttgtaaccct gtaccccgtc
    14161 gccgtttgtc ggaatggtaa gagcctcttt accatcgcgc gcaggaccgt taaggttggt
    14221 tctggcttcg tcgttggtcg cagggatggc cagattgtta ttatcgaagc ttgagttaac
    14281 gataccacct gaagtgccca gctgctggct gctgccagaa agtacccaac ggaagtcata
    14341 attggcggta acgtcagcat cggtacccgc atcaaacttg ccgtttttgt tctcatccga
    14401 gtacagtttg gccacgtagg ttttgccaag ttcgattggc gtgctgccgt tgataaggtt
    14461 gatactggta ccatcaacaa agatggcgac tttcaggttc tggttaacca ccggccctgg
    14521 cgcaacgtta tcgctgtcgc ttccgccgcc gctggcagtg gaaatgtcat aaagatgcag
    14581 cgcagtcccg acgtttggcg taccggtctg cgtggtcggc gtaatttcaa taccgatata
    14641 acgaccacgg tcgtcatcgg taagagtgta actggtcacc ttcgttgcca gctctttcgg
    14701 atcgcccccc tgagcgtcct tgtagctcat ccagcggata gtatcgatag tctttgcgtt
    14761 atcgcccaat cccccaagat ccacgtcgcc ttcggtatca ccaatttccc aacttacggt
    14821 taatttatca cccgagtaaa gacgcttgtc cgtttcactg cctgtcgggc gagcgccttt
    14881 tagatcagaa gtcaacgtta catggtcggc atctgtcgta gcgcttcccc cttccgaata
    14941 gagccacgga atggttccct ggaaaatatt ggtttcgccg ctgtcgaccc aggcgctttc
    15001 ttccgccagc gccacaggtg ccacacaata acctgccagt gccagcgcca atgcgatccg
    15061 ggtcagaccg cgtcttttat cctgcttcat tgatgctctc cttaatgaac cttcagcgac
    15121 tggccgactg gccttacgcc aatcttattt tttggtatac agcacgcgta aaccgtatcc
    15181 ctgcaagcca tctcccgctt gtgcgccata ggtttgcgct gcttcgcggt tagtggccgg
    15241 aatcacaatg tcctcattgg aagtgttcgc ctgtgcaccc acttcaccat tgatgctctt
    15301 gccggtaaat tcccacttgt agtcgtactg cgccatctct tcgtcagtca gtttttcgac
    15361 ctgctggaag acaccgtctt tattgctgtc acgccacaaa acaaatcgat aagtgttgtt
    15421 gagcggcagc ggttctttat ctttacctat caggttgctg ggtttagccg cccccacacg
    15481 atagataaat gcatttagcc caccaatttc tgccccgagg aaagtgacat ccacatagtt
    15541 actgtcgtcg tagggcgcag cttttgcttt ccagcgatag gtgccgggta aggtactgga
    15601 gagggtgaca gtaccctttt ctttatcttg tttagcgata aaaacgcagg ggttgtcggt
    15661 cgagcaaggc ttgttggtta acgtcactcg tttgtcctgc gcgtcgtaaa gccgcggcgt
    15721 gaggttatct tccggcagcg catcgccgtt gcggtcggtg ttatcgccaa agctgtcggt
    15781 tacatcgaga tccatcacca caccatcaac gccatctgcc tgggcgctgg cttcgtggct
    15841 gtggttgttc tcttccggca cgttcgcgat gttattaaca atcaactcaa ttttacggtc
    15901 ctgctgaacg gtgatttcca ccacaccaga gttctgtcgg ttgccctttt catcttccag
    15961 cgccagacgt actcgccagg tattgagttt ttcttgttcg gctttgtcag agctgtactg
    16021 ccaggcgggc agcaccaggt tccagtggtt attgttaccc tctgtcaggg tattctcttt
    16081 gataaccggc ataaccagcg tgcgggcagc attgtttttc gcctgccatg acgcagcgac
    16141 aattttgtag tggtggttga taagcgcgtt aatggtccac ttacattttt ccagcccgac
    16201 cgccgcttca ggcgtgtctt taatgacaca ctcggggtgt tcgttggtgg catccgcttt
    16261 taaccacagg gtgacatcaa gcggatcttt ttctttgtat tccagaacaa taaagttatt
    16321 acgctcaaca aaatcatgac gactgcccat caacgagtga gccgctttca ctttatcgct
    16381 atccaattga tcttttagcg gaacgccgaa ctgataatta agagtggcat taatgctgac
    16441 atcgctgtta tctccggtcc ccgctttata gtccgcccca acggttagta atggcaccgg
    16501 ttgataattc agtccaagtg ttaccgcgta gggatctttc tccaaattat ccgtaccaaa
    16561 aagggcaact tcatcgccgt aatattgctc gaagacaact ttgcccccca gttgcggata
    16621 agaaggtaac caggcttcgg cgcgaatatc ccagccgcgc gcaggacgct cctcataaaa
    16681 atcgaaatct tcggagtctt tccagtcaga aagcggatga taatagttgc cagagaattt
    16741 taaataatca gtccacgctt cgccgcctaa acctaaacgg cgatgtcctc gggtaaaatc
    16801 ataatcataa aatatatttc cgcccaataa ccattcatca aaattatatc ttaccccaag
    16861 accaatattc ccgattgtac gatcttcttt tcgctgcgca gaaaattgac tgaaataaac
    16921 agtggtttga ttatcgtacc agggaacaaa ataatcgatg gaactgccat ccagatcgcc
    16981 gccctggcca atggagaggt tcgaacggac cttaccgtag ggagagagag tgtcttcaat
    17041 ataagattgt gcggttgagg tgatttgact ttgcagatag cttctggcct gagattccag
    17101 cgcctcaggg gtcaagtttt caaacccttg cgtggcggag ttgatgatgt aatcggctcc
    17161 gcgctctttc agcgatttac ccttgttggt ttgttcatcc tgctgtgctg cctggctacc
    17221 taaatcaggt aacgattcgt tttgcttaac atttatctct ggcgccgcga aagcagcacc
    17281 agagataaat gttagaataa tacagcgttt ccatcgcagc atgcactttc cctgtgacaa
    17341 tgatacagtc aaaaataagc tttaatatt
//
LOCUS       DAZGQL010000102        17262 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.102, whole genome shotgun sequence.
ACCESSION   DAZGQL010000102 DAZGQL010000000
VERSION     DAZGQL010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17262
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.102"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..514)
                     /gene="curA"
                     /locus_tag="ACHKIR_004030"
     CDS             complement(<1..514)
                     /gene="curA"
                     /locus_tag="ACHKIR_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HFO9991706.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDIATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGK"
     gene            695..1210
                     /gene="mddA"
                     /locus_tag="ACHKIR_004031"
     CDS             695..1210
                     /gene="mddA"
                     /locus_tag="ACHKIR_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HFO9991707.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERSEPDAI"
     gene            1210..1659
                     /gene="ydcZ"
                     /locus_tag="ACHKIR_004032"
     CDS             1210..1659
                     /gene="ydcZ"
                     /locus_tag="ACHKIR_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO9991708.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSNVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALIGPICGAILLVVGAWLVARRTF"
     gene            complement(1660..1893)
                     /gene="ydcY"
                     /locus_tag="ACHKIR_004033"
     CDS             complement(1660..1893)
                     /gene="ydcY"
                     /locus_tag="ACHKIR_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2526 family protein YdcY"
                     /protein_id="HFO9991709.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREERYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(1979..2152)
                     /gene="ortT"
                     /locus_tag="ACHKIR_004034"
     CDS             complement(1979..2152)
                     /gene="ortT"
                     /locus_tag="ACHKIR_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HFO9991710.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            2347..2442
                     /gene="yncL"
                     /locus_tag="ACHKIR_004035"
     CDS             2347..2442
                     /gene="yncL"
                     /locus_tag="ACHKIR_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HFO9991711.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            complement(2539..3963)
                     /gene="patD"
                     /locus_tag="ACHKIR_004036"
     CDS             complement(2539..3963)
                     /gene="patD"
                     /locus_tag="ACHKIR_004036"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019145 - aminobutyraldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HFO9991712.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKVRAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINVLFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTAPSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTLFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(3985..4779)
                     /gene="ydcV"
                     /locus_tag="ACHKIR_004037"
     CDS             complement(3985..4779)
                     /gene="ydcV"
                     /locus_tag="ACHKIR_004037"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO9991713.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAVYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            complement(4769..5710)
                     /gene="ydcU"
                     /locus_tag="ACHKIR_004038"
     CDS             complement(4769..5710)
                     /gene="ydcU"
                     /locus_tag="ACHKIR_004038"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO9991714.1"
                     /translation="MAMNVLQSPSRPGLGKVSGFFWGNPGLGLFLLLLGPLMWFGIVY
                     FGSLLTLLWQGFYTFDDFTMSVTPELTLANIRALFNPANYDIILRTLIMAVAVTIASA
                     ILAFPMAWYMARYTSGKMKAFFYIAVMLPMWASYIVKAYAWTLLLAKDGVAQWFLQHL
                     GLEPLLTAFLTLPAVGGNTLSTSGLGRFLVFLYIWLPFMILPVQAALERLPPSLLQAS
                     ADLGARPRQTFRYVVLPLAIPGIAAGSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQ
                     QGAIGNMPMAAAFTLVPIVLIALYLAFVKRLGAFDAL"
     gene            complement(5711..6724)
                     /gene="ydcT"
                     /locus_tag="ACHKIR_004039"
     CDS             complement(5711..6724)
                     /gene="ydcT"
                     /locus_tag="ACHKIR_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO9991715.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGKSASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVDKKQRYAMAQEALEKVALGFVQQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDALMADKLCGMTGSFALRPEHIRFNIPGEVQANGT
                     IQAVQYQGAATRFELRLNGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVPLV
                     EER"
     gene            complement(6742..7887)
                     /gene="ydcS"
                     /locus_tag="ACHKIR_004040"
     CDS             complement(6742..7887)
                     /gene="ydcS"
                     /locus_tag="ACHKIR_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter substrate-binding
                     protein YdcS"
                     /protein_id="HFO9991716.1"
                     /translation="MSKTFARSSLCALTMTIMTAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMAKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKALDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGIRDPYQLTEEQY
                     QAVLKVMRDQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMSWSLTPKVQGDVAAWFGSLPVVPQGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            complement(8132..9538)
                     /gene="ydcR"
                     /locus_tag="ACHKIR_004041"
     CDS             complement(8132..9538)
                     /gene="ydcR"
                     /locus_tag="ACHKIR_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFO9991717.1"
                     /translation="MKKYQQLAEQLREQIASGIWQPGDRLPSLRDQVALSGMSFMTVS
                     HAYQLLESQGHIIARPQSGYYVATQAIKMPKAPVIPVTRDEAVDINTYIFDMLQASRD
                     PSVVPFASAFPDPRLFPLQQLNRSLAQVSKTATAMSVIENLPPGNAELRQAIARRYAL
                     QGITISPDEIVITAGALEALNLSLQAVTEPGDWVIVENPCFYGALQALERLRLKALSV
                     ATDVKEGIDLKALELALQEYPVKACWLMTNSQNPLGFTLTPQKKARLVALLNQYNVTL
                     IEDDVYSELYFGREKPLPAKAWDRHDGVLHCSSFSKCLVPGFRIGWVAAGKHARKIQR
                     LQLMSTLSTSSPMQLALVDYLSTRRYDAHLRRLRRQLAERKQRAWQALLRYLPAEVKI
                     HHNDSGYFLWLELPEPLDAGELSLAALTHHISIAPGKMFSTGENWSRFFRFNTAWQWG
                     EREEQAVKQLGKLIQERL"
     gene            complement(9617..10033)
                     /gene="hicB"
                     /locus_tag="ACHKIR_004042"
     CDS             complement(9617..10033)
                     /gene="hicB"
                     /locus_tag="ACHKIR_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HFO9991718.1"
                     /translation="MRYPVTLTPAPEGGYVVSFVDIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPLTNNDHFIEVPLSIASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            complement(10077..10253)
                     /gene="hicA"
                     /locus_tag="ACHKIR_004043"
     CDS             complement(10077..10253)
                     /gene="hicA"
                     /locus_tag="ACHKIR_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin HicA"
                     /protein_id="HFO9991719.1"
                     /translation="MKQSEFRRWLESQGVDVANGSNHLKLRFQGRRSVMPRHPSDEIK
                     EPLRKAILKQLGLN"
     gene            10475..10705
                     /gene="yncJ"
                     /locus_tag="ACHKIR_004044"
     CDS             10475..10705
                     /gene="yncJ"
                     /locus_tag="ACHKIR_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2554 family protein"
                     /protein_id="HFO9991720.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFMWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGHR"
     gene            complement(10797..12758)
                     /gene="rlhA"
                     /locus_tag="ACHKIR_004045"
     CDS             complement(10797..12758)
                     /gene="rlhA"
                     /locus_tag="ACHKIR_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HFO9991721.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLEQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLTRAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHLDV
                     AVTEPLANGDGLNVMIKREVVGFRANTVEKTGENQYRVWPNEMPAELHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTIYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVYPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLPKRKG"
     gene            complement(12831..13367)
                     /gene="sutR"
                     /locus_tag="ACHKIR_004046"
     CDS             complement(12831..13367)
                     /gene="sutR"
                     /locus_tag="ACHKIR_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HFO9991722.1"
                     /translation="MENLTRFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSATPSVYDPQQQVMVITSLFPYDPQLCFEHF
                     SIQMAPGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQSLNCGEGARFAADVTHVYR
                     NGGEQIVHFHSLIHYPRS"
     gene            13420..14631
                     /gene="ydcO"
                     /locus_tag="ACHKIR_004047"
     CDS             13420..14631
                     /gene="ydcO"
                     /locus_tag="ACHKIR_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012601493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HFO9991723.1"
                     /translation="MLRYLYAITHEVTMRLFSIPPPTLLAGFLAVLIGYASSAAIIWQ
                     AAIVAGATTAQISGWMTALGLAMGVSTLALTLWYRVPVLTAWSTPGAALLVTGLQGLT
                     LNEAIGVFIVTNALIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDDQ
                     FTLCGSMLLVWLATKAVAPRYAVIAAMIIGVVIVIAQGDVVTTDVVFKPVLPTYISPD
                     FSFAHSLSVALPLFLVTMASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALFFSPFGVY
                     SVGIAAITAAICQSPEAHPDKDQRWLAAAVAGIFYLIAGLFGSAITGMMAALPVSWIQ
                     MLAGLALLSTIGGSLYQALHNERERDAAVVAFLVTASGLTLVGIGSAFWGLIAGGVCY
                     VVLNLIANRNR"
     gene            complement(14666..15334)
                     /gene="ydcL"
                     /locus_tag="ACHKIR_004048"
     CDS             complement(14666..15334)
                     /gene="ydcL"
                     /locus_tag="ACHKIR_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HFO9991724.1"
                     /translation="MRTTSIAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     TSATGKPVLRWVDPSFDQSKYDSIVWNPVTYYPVPKPSTQVGQKVLDKILSYTNTEMK
                     EAIAQRKPLVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTM
                     DTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFD
                     VNKK"
     gene            complement(15673..16266)
                     /gene="tehB"
                     /locus_tag="ACHKIR_004049"
     CDS             complement(15673..16266)
                     /gene="tehB"
                     /locus_tag="ACHKIR_004049"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HFO9991725.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLENLHTRVVDLNNLTFDGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWEMVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            complement(16263..17255)
                     /gene="tehA"
                     /locus_tag="ACHKIR_004050"
     CDS             complement(16263..17255)
                     /gene="tehA"
                     /locus_tag="ACHKIR_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HFO9991726.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLIRTERAVLMKAEDKE"
BASE COUNT         4300 a         4539 c         4175 g         4248 t
ORIGIN      
        1 gtttgccgat ttgacccacc gtcgccccca caggacctgt cgccgcagca acaaccaatg
       61 tttcgccctc tttgggctga ccgatatcca gcagccccat ataagcggta aagcctggca
      121 tccccagcac acccagcgac cacgatggat tttgcggatg atcgccaagt ttcaccagat
      181 catcaccact ggatatgtca tagtcctgcc atccactgta gcccagcacc cagtcgccag
      241 gctggtaatc aggatgattc gactccacaa cacggctcac cgtaccgccg accattacgc
      301 caccaatatc aaccggcggt gaataagatg gctcatcgct catacgtcca cgcatatacg
      361 ggtccagtga caaataaact gtgcgcagta acacctgtcc ttcacccggt gtggcgatat
      421 catcttcttc aagacggaaa ttctccggaa ccggcgcgcc atgcggacgc gaggccagaa
      481 cccaacgtcg attacgctgc ttttgttgcc ccataatgct ctcctgtgct tttggcatga
      541 atccatcaat agtagcaggc tcgcaggaat ggttttgttt gcagccgtca catcccctct
      601 gcaacacgca ataatttcat cgaggaatcc gttgcttcgg gctgttttcg atagtattga
      661 taacgtcatc atcattattt aatcgaggcc aaacatgtcc atccgttttg cccgcaaagc
      721 cgactgtgct gccattgcgg aaatttataa ccacgccgtg ttgtatacgg cggctatctg
      781 gaatgaccaa acggtggatg cagataaccg cattgcctgg tttgaagcgc ggaccatagc
      841 aggttatcca gtgctggtga gcgaagaaga tggcgtagtg acgggatatg cctcgtttgg
      901 cgactggcgt agtttcgatg gttttcgcca taccgtggaa cattcggttt atgtccatcc
      961 cgatcatcag ggcaaaggtc tggggcgtaa attgttaagc cgattgattg atgaagcgcg
     1021 ggattgcggg aagcatgtca tggtcgccgg gatcgaatcg caaaatcagg cgtctttgca
     1081 tctgcaccag tcgctgggat ttgtcgtcac cgcgcaaatg ccgcaggtgg gcactaaatt
     1141 tgggcgctgg ctggacctga catttatgca gttgcaactc gacgaacgca gtgaaccgga
     1201 cgcaatctga tgaatcagtc gctcaccctt gcttttttga ttgccgccgg gattggtctg
     1261 gtagtccaga acacgctgat ggtgcgcatc acccagacct cctctaccat tctcatcgcc
     1321 atgttgctga actctttggt aggaattgtg ctctttgttt ctattttatg gtttaaacaa
     1381 ggtatggcgg ggtttggcga actggtgtcc aacgtgcgct ggtggacact tatacctggc
     1441 ttgctgggat cgttttttgt cttcgccagt atcagcggat atcagaatgt gggagcggcg
     1501 acaactattg cggtactggt cgccagccag ctgattggcg gtctggtgct ggatatcttc
     1561 cgcagccatg gcgtaccact acgggcgttg atcggcccaa tttgtggcgc aattttactt
     1621 gtggttggtg cctggctggt ggccaggcgc acattttaat cagaggatgg tgccaccttt
     1681 ggttaactgt tcgtggcgcg cttccatatc ttctttatgt ttgcgaccgt gatgggcgat
     1741 cgctgtgcga agacgctgct gctgggtata gcgttcttcc cgacttaact ccgcatcatc
     1801 gctcagggcg attaataatt cgttcatatg ggcaatgacg ctctcttcaa tcgcggcatc
     1861 cacgcgcgcg atgacttcat ccagatgcga catactttct ccttagccac ttgtgaaatt
     1921 tatcgattca tccaaatgat ataagtctgc gtggtgccgt caagccagtg agtaagattt
     1981 aaaacagtga aaacaacagc gccaccggga aactcatcgg ccatgttgcc cccaccagaa
     2041 aggcgcttaa gaaacggatg cgtttcttat cgtgagaaag aaaccaggtg ataagaaatg
     2101 cgattgctgc cataaccgca taaaaaacaa gcatgtgttg atagagagac atgtacgggc
     2161 gtccagccag atgaataaaa cgcgcgcaaa tatgcgtcat tttttgacgt acatcaagtt
     2221 ttttaaccgg tttgagaaaa aaacatcaaa cagcaacttg cccttatcca tcttcagaat
     2281 gagtattatt gcggattttc ttaacccgta ctatacacat aggggcaatg ataaaaggtg
     2341 gcaaaaatga atgtttccag tagaactgta gtactgataa atgtctttgc tgctgttggt
     2401 ttgtttactc ttatctctat gagatttggc tggtttattt gatgtagtga ttttgcacgc
     2461 agcacgtact gtcagagaaa tcagtgctct gacagtacgc aagatcatct catactctta
     2521 cgatctgcaa tattatcctt aatgtttaac catgacgtgg cggacgacgg tgtaatcctc
     2581 cagcccataa agtgacatat ccttgccgta accagaaagt ttctgcccac cgtgcggcat
     2641 ttcacttacc agcatgaaat gggtattgac ccaggtacaa ccatattgca gccgtgcgct
     2701 gacgcgatgc gccctgccca catctttcgt ccataccgaa gatgcaagtc cgtactggct
     2761 gtcattcgcc cagtttacca cctgttcttc gttgtcgaag agtgtaacac tcactactgg
     2821 accaaatacc tctttttgca cgatggcatc gtcctgtaat gcgccagcca gcagcgtcgg
     2881 cgcatagtaa tagccattac ccttgcgctt ttcaccgcca gtgatcactt tgatgtgccc
     2941 tgtcgctttc gcctcttcta ctgccttgct gacgcgttcg agatgcgcca gcgagcttaa
     3001 aggtccaagc tccgtagact cgtcatccgg cgcaccagat tttaacgttg ccaccgcagc
     3061 acccagtttt tccaccagcg tatcgtaaat gcctttttgc gcgtagatcc gacaagccgc
     3121 agtacaatcc tgtccggcat tgtaatagcc aaatgtacgt acaccttcga ccactgcctc
     3181 aatatccgca tcatcaaaaa caatcactgg cgctttgcca ccaagttcca tatgagtacg
     3241 ctttatggac ggcgcggtat ggctgatgat gtgttcccca gtggcgatag agcccgtcag
     3301 cgacaccatc cgcactttgg gatgaccggt cagcggatcg cccaccgttt tgcctctgcc
     3361 aaacagtaca ttaatcacgc cagccgggaa gatatctttc gccaactctg ccaacttcaa
     3421 cgctgtcagc ggggtaattt ctgatggttt aagcactacg cagttccctg ccgccagcgc
     3481 cggagccagt ttccacgcag ccatcatcag aggataattc cacggtgcga tagaagccac
     3541 gacccccaac ggatcgcgac ggatcatcga agtatgacct tcaagatatt cacctgccgc
     3601 cagaccattc agacagcgcg ccgcaccagc gaaaaagcga aaaacatcga caatcgccgg
     3661 gatttcatca ttgaacgcac tatgcagcgg tttgccacaa ttacgggact ccagttcggc
     3721 aaaaacctga ccattttctt cgataacatc agccagtttc agcagacatt ccgcacgcac
     3781 tttcggcgtg gtttgccccc attcggcaaa tgctgcatct gccgcgcgaa cagcagcatc
     3841 gacctgctct gcggatgcct cggcaatctc cagtaaaacg tcccccgttg ccggattata
     3901 gacaggctgt ttttcccctt cgccgctaac cagttctccg ttaatcagta acttatgttg
     3961 catagcatat tcctgtatca gtggttattg accattgtcg ccttcgcggg ttagccacca
     4021 ggcccccagg atcggcaagg ttgttaccaa cataaccagc agtgccacca cgttagttac
     4081 cggtacatca cgcggtcgcc caagctgatt gagcaaccac aacggtaacg ttcgttcatg
     4141 acctgccgta aaggtcgtaa cgatgatttc atcgaacgac aaggcaaacg ccagcattcc
     4201 tcctgccagt aacgccgaac tgagattcgg caacactacg tagcggaagg tttgccagcc
     4261 attagcccca agatccattg acgcctcaac cagactccac gaggtgcggc gaaaacgggc
     4321 gatgacattg ttaaagacca ccactacaca aaaagtcgca tgaccgacca cgatggtgaa
     4381 aaatcccggc tccagattga tggttttaaa ggcggttaat aacgccagac cagtgacaat
     4441 gcccggcagc gcaatgggca gcagcagtaa cagcgaaatg gcgtttttgc cgaaaaagtc
     4501 tcgtcgccac agcgcggcag cggccagcgt ccctaacacc agcgcgatta atgtcgccag
     4561 cgccgccact ttaagtgaca gtgtcacggc atcaagaata tcactacgct gtgctgccac
     4621 gctaaaccag cgcagcgtca ggccttgcgg tggaaaacta aacgccgcat cttcagtgtt
     4681 aaacgcataa acggcgataa tcagaatggg aaaatgtaag aaaaccacgc cgccccaggc
     4741 cgccagtttg aggaaaaacg gtgcgcgttc agagtgcatc gaacgctccc agacgtttca
     4801 cgaacgctag gtaaagtgcg atgagtacaa tcggcactag ggtgaatgcc gccgccatcg
     4861 gcatattgcc aatcgccccc tgctgggaat aaaccatatt gccgataaaa tatcctggag
     4921 ggccaaccag ctgcgggaca ataaaatcgc ccagcgtgag tgagaaggta aagatagagc
     4981 cagcggcaat acccgggatt gccagcggca gcaccacata gcgaaaggtt tgtcgtggac
     5041 gtgcgccgag atcagctgac gcctgcaaca atgacggcgg caaacgctca agcgccgcct
     5101 gcaccgggag gatcatgaac ggcaaccaga tatagagaaa caccagaaag cgccccagcc
     5161 cggaagttga cagcgtatta cctcccaccg ccggtagcgt taggaacgca gtcagcagtg
     5221 gttccagccc cagatgttgt aaaaaccact gcgccacgcc atcttttgcc agcaataacg
     5281 tccaggcata ggctttcaca atgtagctcg cccacatcgg caacattacc gcgatataaa
     5341 aaaacgcttt cattttcccg ctggtatagc gcgccatata ccacgccatt ggaaaagcca
     5401 gaatggcgct ggcgatagtg actgccacag ccataatcag cgtgcggaga atgatgtcgt
     5461 aattcgccgg attaaacagc gcacggatat tcgccagcgt cagttccggc gttaccgaca
     5521 tggtgaaatc gtcaaaggta taaaatccct gccataacag tgtcagcagc gagccgaaat
     5581 agacaatgcc aaaccacatt agcgggccaa gcagcagtaa aaacagcccc agccccggat
     5641 taccccagaa gaaaccggac accttaccca gacctggacg tgaaggtgat tgcaatacgt
     5701 tcatcgccat tcacctctcc tcaaccagcg gtaccatcac atcacgcgac caggaaacca
     5761 tcacctgttg tccgggcgtg agcgtggcgg gcagttcttc gcctgtcata ttggcctgac
     5821 tcaccagcag tttttcaccg ccgttcagtc tcagttcaaa acgggtcgcc gcgccctgat
     5881 actgcaccgc ctggatcgtg ccattagcct gcacttcgcc ggggatgttg aagcggatat
     5941 gttccggtcg cagggcgaag cttcccgtca tgccacaaag tttgtctgcc atcagtgcat
     6001 caaaaacatt cgatgtcccg acgaacccgg caacaaacgg cgtgcgcggg cgcatataga
     6061 gatcacgagg ggaatcgacc tgctcaatgc gtccattatt gaaaaccgcc acacgatcgg
     6121 acatcgataa cgcttcgccc tgatcgtggg taacgaagat aaaagtgata ccgagagact
     6181 gttgcagttt tttcagttcc agctgcatct gctcacgcaa tttgagatcc agtgcgccga
     6241 gcggttcatc caacagcaat acgcgcggtt cattcaccaa tgctctggcg atagcaaccc
     6301 gctggcgctg accaccagaa agctgtgacg gcttacgttg ctgaacaaaa ccaagcgcca
     6361 ctttctccag cgcttcttgc gccatagcat atcgctgttt tttatccacg cctttgacca
     6421 tcagcccata ggccacgttg tcaagaatcg acatatgcgg aaaaagtgcg tagtcctgga
     6481 agacggtatt aacatcccgc tcccacggtg gcagattgct ggcggattta ccaaagatag
     6541 agatagcccc gccggaaagc tgttcgaagc cagcaatcag gcgcaggcag gtggttttgc
     6601 cggagccgga cggccccagc atagagaaga actcaccatc ttttatcgca atactgacgc
     6661 catctactgc gcgcacgtca ccgtacaacc gtgagacgtt gtcaaactcc actgcgtacg
     6721 tcataaaaca ccccagcgaa gttagcgacc gcccataatg gcaatgtaat cctgcgtcca
     6781 gcgactgtag ggaacaaact tgcccccttc tgctataggc gttttccaga aggcgatttt
     6841 atcgaaatag ttaaaaccgt tggtttcaca acctttttca cctaataacg gactggcttt
     6901 acacccttgc ggcactactg gtaacgagcc aaaccaggcc gccacatcac cctgcacttt
     6961 tggcgttaat gaccagctca tccatttgta ggcgcaaacc ggatgtttcg cttcgctatg
     7021 tagcatggtg gtgtcagccc aaccggtaac gccctccttc gggaaaacgg tagcgacagg
     7081 ctggccttcg gctttcaggg cgttggcctg atagggccat gcactggaag caaccacacc
     7141 ctcgtttttg aaatcgctca tttgcacggt agtgtcatgc cagtagcgat ggatcaaact
     7201 atgttgatcg cgcatcactt tcagtaccgc ctgatactgt tcttcggtga gttgatacgg
     7261 atcgcggatg cccaactgcg gctgagtggc tttaacgaac aacgcagcgt ccgcaatgta
     7321 gatagggcca tcataagcct gaacgcggcc tttattgctc ttgccgtccg gcagattctg
     7381 ctcaacaaaa accacttgcc agctatccgg cggcgtcggg aaggttttag tgttgtacat
     7441 cagcaggttc ggcccccatt ggtaaggtgt gccgtaaact ttgccgccaa cgttaaacca
     7501 gtcgccttta accacgcgcg gatcgagcgc tttccagttg ggaatcaagg cggtattaat
     7561 tggctgcacg cgtttaccca taatcaaacg cagcgaggca tcgccggatg ccgtaaccag
     7621 atcgtaaccc cctttggcca tcagactgac catttcatcg gaagtcgcgg cggttttaac
     7681 attcaccgcg cagcctgtct ctttttcgaa ttgcgttacc cagtcgtatt gtttatcagt
     7741 ttgtccgcgt tcgatgtatc ccggccaggc gataatatcc agtcgccctt ccggtttatc
     7801 taaattggta ggtggttcgg cggcgtgagc ggtcattatt gtcatggtga gcgcacacag
     7861 gctgctgcgg gcaaatgtct tgctcataaa gtcttactcc tgtcgtaatt aattgttcag
     7921 ccgccgtgcc gtaaaaatat ctccttgttt aaccatagac ggcggctagc aggcacacct
     7981 tcgcccctga gaaaatttaa tcaggttgtg agctaccgcg cattatgccg tatgtaaggt
     8041 tatcttctgg agtatagaca aggagttagg aaatatgact gctattcgaa attcatatta
     8101 cccaacctga gaggaattta attaaacgct attacagccg ttcttgaata agtttgccta
     8161 attgtttaac cgcctgttct tcacgttctc cccactgcca cgcggtatta aaacggaaaa
     8221 aacgtgacca gttttcaccg gtagaaaaca ttttacccgg cgcaatactg atatgatgcg
     8281 tcagtgctgc caggcttaat tcgcccgcat ctaacggctc ggggagctcc agccagagaa
     8341 aataaccgct gtcattatga tgaattttca cttccgcagg cagataacgc agcagtgctt
     8401 gccaggcacg ttgtttacgt tccgcaagct ggcgacgcag gcgacgaaga tgggcgtcgt
     8461 atcggcgcgt ggaaaggtaa tccaccagcg caagctgcat cggtgagctg gtggaaagcg
     8521 tactcatcaa ctgcaagcgt tgaattttac gtgcatgttt tccggcggcg acccaaccaa
     8581 tacgaaaacc aggcaccaga catttcgaaa acgaagagca atgcaaaaca ccatcgtggc
     8641 gatcccacgc tttcgcaggc agcggttttt cccgtccaaa ataaagttcg ctgtaaacgt
     8701 catcttcaat cagcgttacg ttgtactgat tgagcaacgc taccagccgc gcttttttct
     8761 gcggcgttaa ggtaaatccg agtggattct ggctattagt catcagccag cacgctttca
     8821 ccggatactc ctgcaacgcc agttccagcg ccttgagatc aatcccttct ttaacgtccg
     8881 tcgccaccga taacgccttc agccgtaacc gctccagcgc ctgcaacgca ccgtagaaac
     8941 aaggattctc tactatcacc caatcgcccg gttcagtgac cgcttgtaaa ctgaggttta
     9001 atgcctctaa cgccccggca gtaatgacaa tttcatcggg tgaaatggtg atgccctgta
     9061 aggcatagcg acgagctata gcctgacgca gttcagcgtt tcctggcggt aagttttcaa
     9121 tcacgctcat cgccgtggcg gttttgctta cctgtgccag cgagcggttg agttgttgga
     9181 gggggaaaag tcgcgggtcg ggaaaggccg aggcaaacgg aactaccgac ggatcgcgac
     9241 tggcctgcaa catatcaaaa atataggtgt tgatatcgac tgcttcatct cgagtgactg
     9301 gaatgactgg cgctttcggc atttttattg cctgtgtcgc aacgtaataa cccgactgcg
     9361 gtcgcgcgat aatatgtccc tgactttcga gcaactgata ggcatggctg acagtcataa
     9421 agctcatgcc agaaagcgcc acctggtcac gcaacgaagg caaacgatcg ccaggttgcc
     9481 agatacccga cgcaatctgc tcgcgtaatt gttctgcaag ctgctggtat tttttcatta
     9541 tcgaatcgta attatgtgca gatgattcgg cagtctatat cagttgttaa aaatgacaac
     9601 ttttcattaa ctgttatcaa accatcacca gtgataactc tttgccaagc gccttagccg
     9661 caagctggac ggcgtcgatt tttgtagcat ggtgcaagtt aaataagcga gtaatctcct
     9721 gtttaggttt gccaattcgt ctggctaact cttgctgagt gatttctgac tgtaaaaaag
     9781 catttaacag caatacttta gaggcgatac ttaaaggcac ttcaataaag tgatcgttgt
     9841 tagttaatgg cgaaggcaac gggattagct cattatcttc aaaataaaaa tcaaatgcgg
     9901 tgagtaaggc atcttttgct gcttccatcg cttcagcaac agtttctccc tgcgtcaacg
     9961 cttcagggat atctacaaaa gaaaccacat aaccgccttc cggcgcagga gtaagagtga
    10021 cgggataacg cataattttg actgtctctc gcatgtcatt aaccttctta tggcgattaa
    10081 tttaaaccga gttgcttcag gattgcttta cgcaatggtt cttttatctc atcactgggg
    10141 tgacgcggca tgacactgcg tcgcccttga aacctgagtt tcaaatggtt gctgccattc
    10201 gccacatcga cgccctgaga ttcgagccaa cgtctgaact cgctttgttt cacatcgcct
    10261 ccctgccgct gagtatatca acaaagtaaa cattagtgtt tacctccgtg tgtaatttgc
    10321 tcgaaaatag cactaaatcg ccgcaacggc agaatgcaat gcaaaatcag gaaggaagat
    10381 tccagaattt ctttgcgcta atttgcaaaa ctgaataata acctcaagaa acgatcggca
    10441 tcatagcgac aaaaggtaag aaaaaggtgg caggatgttt acgaaggcgt tatcggttgt
    10501 cttattaacg tgtgcactgt tctcaggaca actcatggca gggcacaaag gacatgaatt
    10561 tatgtgggta aagaatgtgg atcatcagct gcgtcatgaa gcggacagcg atgaattgcg
    10621 tgcagtggcg gaagagtcgg cggaaggttt gcgcgagcat ttttactggc aaaaatcgcg
    10681 caaaccagaa gcgggacatc gttgagtcat ttgttgccgg atgcggcatg acgccttacc
    10741 cggcaggcaa tatcatgcag atttgatgag ttgtgagtaa ccagaaaact ggcgttttac
    10801 ccttttcgct tcggtaaagt tttcagcagc tcatccggac ttacggatgc cactacgctt
    10861 cccggtaacg gcattttcag tatgtgattt ttgattttgc caatgacgtg catctcgcat
    10921 gggcggcaat caaactttag cgttaatact tcatcgccgt taaccagttg cattggcgtc
    10981 gccttccagc ttttgatatt gcctttcgcc tgtttcgggc acagattgaa ggcaaagcgc
    11041 agacaatgct tagtgatcat caccgggact tcgcccttct cttcatgcgc ttcatacgcc
    11101 gcatcaatca gttgcacacc atagcgatga taaaattcac gcgctttctg gttgtaaacg
    11161 ttcgcgagga aactcaaatg cgtttgcgga taaaccggcg caggatcagc aaccggttta
    11221 cggctgccgc gctggtaact ggcaagacgc gcagcatcta gcatgtcggc ggcttcacgg
    11281 cggaactggt ttaacagact gtttggtaca aacagcgcac ccggcaaatt aatttgcaca
    11341 tcgcgggcgt aatagatggt ttgccccagt tttgccagac catcctttag attgttcatt
    11401 gctttttcgg cgttattggc ttcgtcgaac tgcccgtcca gcgtatgcgt gatgctgaca
    11461 ccctcttcac tggtgagggt cagaatcagt tgttcctgcc agccgcccag ttcaatgtct
    11521 accgccaccc gacgttcgct ggaggttttt gtcagtgcct gctgccagtt atgatcaaga
    11581 ttacggttta gtgggtgatg cgggcgaatt ttgtgcaact ctgctggcat ttcattgggc
    11641 cagacgcggt actgattttc tccggttttc tcgaccgtat tggcacgaaa gccgacgact
    11701 tcacgtttaa tcatcacgtt caggccatcg ccatttgcca gtggctcggt aacggcaaca
    11761 tcgagatgat ctttcgccac tttcaatact tcacccaccg gcaggccgat aaatttcggc
    11821 gaatcgaacg cgccaatatc gcctttacgg gcattcacaa aataatctgt gctaccacgg
    11881 tggaaagtct tttccgtcga tggaacaaag aaatgttcag tacgacctga tgaagcacgc
    11941 gtcagatcgc cacgttcttc aataatggca tccagcatct ggcgataatg ggcggtgata
    12001 ttcttcacgt agctcatatc tttgtaacgc ccttcaatct tgaaggagcg tacgccagca
    12061 tcaatcagcg cgccgaggtt ggcagtctga tcgttatctt tcatcgacag cagatgtttt
    12121 tcataggaaa ccacccgccc ctgatcgtct ttcaatgtgt atggcaaacg gcacgcctgc
    12181 gagcaatcgc cacggttggc gctacgccct gtttgcgcat gagaaatgta gcactgaccc
    12241 gaataggcca cgcacagtgc gccatgaata aagaattcaa tggtcgcgtc cgtggcctgg
    12301 tgaatcgcgc ggatctgctc aagattcagc tctcgcgcca gcacaatctg cgtgaagcca
    12361 acatcagaga ggaacttcgc tttttctact gtacgaatgt cgcactgcgt actggcgtgc
    12421 agttcaatcg gcggaatatc aagttccaga atccccatat cctgaacaat cagcgcgtcg
    12481 acgccggtct ggtagaggtc ggtaatcagc cgttgcgcag gttccagctc atcatcatgc
    12541 aaaatggtgt taagcgtgac gaaaattttt gcaccataac gatgggcaaa tggcaccagt
    12601 tcggcaatat ctttcaggct attactggca ttatgacggg caccaaaacc agggccgccg
    12661 atataaacag catcggcacc gtgcaaaata gcttcgcggg caatggcggc atcgcgtgcc
    12721 gggcttaaca gttcaagtcg atgagaagat acggtcacat tcttatccag tagctgaaaa
    12781 atggcggcta ttttagccac aagcgcgacg ttgcgaaata gttttcccgc ttaactgcgt
    12841 gggtaatgga tgagggaatg aaaatgtacg atttgctccc caccattacg atagacatgc
    12901 gtaacgtctg cagcaaatcg agcgccttct ccacaattaa ggctctgcca ttcaccatca
    12961 acgcacagat caagttgccc atcaatgacg acaacatgtt cgattactcc cttctcatgg
    13021 ggcgtcgatt cacttattgc accaggtgcc atctgaatcg agaagtgttc aaagcagagt
    13081 tgtggatcat aaggaaacag cgaagtgatc accatcacct gctgttgcgg atcataaact
    13141 gaaggcgtag cggactgcgg cggagaaata aatgtcgaaa atggcacatt caacccggtg
    13201 gcaattttcc ataatgtcgc gaccgtcggg ctggactcat tacgctcaat ctgccctaac
    13261 atcgctttag aaacgcccgt cgcttctgcc agtcgcgaaa gactccagcc gcgctgctga
    13321 cgtagttgtt taagtgtggt ggataaaaag cgagtgagat tttccatgcg actcctccgg
    13381 caaaacggaa gtttatcact tgtgcgttat aacggacaaa tgctacggta cctgtatgct
    13441 ataacgcacg aggtgaccat gcgtctgttt tctattcctc cacccacgct actggcgggg
    13501 tttctggcgg tattaattgg ctacgccagt tcagcggcaa taatctggca agcagcgatt
    13561 gtcgccggag ccaccactgc acaaatctct ggctggatga cggcgctggg gctggcaatg
    13621 ggcgtcagta cgctggctct gacattatgg tatcgcgtac ctgttctcac cgcatggtca
    13681 acgcctggcg cggctttgtt ggtcaccgga ttacagggac taacacttaa cgaagccatc
    13741 ggcgttttta ttgtcaccaa cgcgttaata gtcctctgcg gcataacggg actctttgct
    13801 cgtctgatgc gcattattcc gcactcgctt gcggcggcaa tgcttgccgg gattttatta
    13861 cgctttggtt tacaggcgtt tgccagtctg gatgatcaat ttacgttgtg tggcagtatg
    13921 ttgctggtat ggctggcaac caaggccgtt gcaccgcgct atgcggtaat tgccgcgatg
    13981 attattgggg tcgtgatcgt tatcgcgcaa ggtgacgttg tcacaactga tgttgtcttt
    14041 aaacccgttc tccccactta tattagccct gatttttcgt ttgctcacag cctgagcgtt
    14101 gcactccccc tttttctggt gacgatggca tcgcaaaacg caccgggtat cgcagcaatg
    14161 aaagcagctg gatattcggc tcctgtttcg ccattaattg tgtttactgg attgctggca
    14221 ctgttttttt cccctttcgg cgtttattcc gtcggtattg cggcaatcac cgcggctatt
    14281 tgccaaagcc cggaagcgca tccggataaa gatcaacgtt ggctggccgc tgccgttgcc
    14341 ggcatttttt atttgatcgc aggtctgttt ggtagtgcca ttaccgggat gatggctgcc
    14401 ctgcccgtaa gttggatcca gatgctggca ggtcttgcgc tgttgagtac cattggcggc
    14461 agtttgtatc aggcgctgca taatgagcgt gagcgcgacg cggcggtggt ggcatttctg
    14521 gtaacggcaa gtggattgac gctggtcggg attggttctg cgttctgggg attaattgcc
    14581 ggaggcgttt gttacgtggt gttgaattta atcgctaaca gaaaccgata ataaaaagcg
    14641 ccatctaacg atggcgcaac tggtattact ttttgttaac gtcaaacatg gtggcatcgg
    14701 tcgccatgtc atcaataact tgtttaatat tttcgaaagc cattggcgta ctttcgttat
    14761 tcaggtcttt accttcgccc tgacgaacga ctttgataac cggtttatta gtcgctgcat
    14821 caatcagctc accttcaaaa tacaggcgag tatccatggt acggtggccc gtcgccattt
    14881 gcgtccccgc aaccactaat gcaacaggaa ccacttcata gaattgcagc ccttctttgc
    14941 tggtatctac accggtaatg gccccacgga aaatcagact acgcggccca gcggtggtaa
    15001 ccagtggttt acgctgcgca atcgcttctt tcatttcggt gttggtataa ctcaaaattt
    15061 tatccagaac tttctgccct acctgggtag atggtttcgg taccggataa taagtgactg
    15121 ggttccagac gatgctgtca tatttgcttt gatcaaaact cgggtctacc caacgtaaaa
    15181 caggtttacc tgttgctgag gttgtttctt ttaaatcaga gtaattgttt aaaaaaccag
    15241 aatatttatc tggctgggtg attttagagg cacaaccaga cagagccaac aagccgcata
    15301 aagctgcaac tttcgcaatt gatgtggtac gcatgaatag tttcctgaga tgattatatt
    15361 actttctaag ttatagcaaa aagcgccgct tcgtgttgtg gcaaaaaaag tgttggtgaa
    15421 aataaatttt ttagcccctt ttcagacgac atagtcaatg ccgaaaaaga catagtcaat
    15481 gccgaaaaag gggctgccga aaaaggggct aagtcaatca tcagtagtta aatttcagta
    15541 agttaagctg ctaaaaaatt tcgactactg tgattaattt cacatccatt cgataaaaaa
    15601 tatgcttacc gaaaagaaga acagtaaacc atctcagcgt ggggaatttt cacgcgcctt
    15661 gttcctagcg ggtcattttt tacgtgccag catcgtggcg aaacgcagtt taatacgatt
    15721 accgttggcg tcggtgcggt gcagctcgcc gacgtcttca ttgtatttca ccatctccca
    15781 gccttcgtaa tatcgacgta attccccctc tttgaaggca aacgggaagc cgacggtaca
    15841 tggataatca gcggtatcca tcgccgccac aatcaggttg taaccgccag gtttagtgca
    15901 acgttgcata ttggcaatca gccctgggat ggttttagcc tcaaggaaca tcagcaccac
    15961 agtcgaaaga ataaaatcat actgtccatc aaatgtgagg ttattcagat caacgacccg
    16021 ggtgtgtaaa ttctccagat tttcaatgga tttaatccgc tcgacgttgg cgatactcat
    16081 ggcattttta tcccatgcgt caacatcata accattggct gccaggtaaa gactgttacg
    16141 accattgcca cagcccagat ccagcgtttt acccggttta accactttca ccgcttccag
    16201 cacttcagag tgtgtgcgag ttaattcata tttatcagta aaatagtttt cgtcacgaat
    16261 gatcattctt tgtcctctgc tttcattaaa acggcgcgct cggttctgat taacaatttt
    16321 ccctgcatca gaagcgcaaa agtacggatg agcagtattg caataataaa attggtaaag
    16381 ataaacagcg gcaccgccag cgtatggaaa aatccattat cgctgccact ccccagatgc
    16441 aaaccggtgg ttgccagtgc agatacgccg aacgagaaac tccagaatga agcattaaat
    16501 ggctgggaga gataccatgg catcagacgt agcataaaca gcagttgcag cagtccataa
    16561 ccgaaaagca ttttcgccag cgtgtcaccc tcgccgccgt tgacgctcag ccaggcacta
    16621 caggccacca gcgcaggagc gagctgaatg ccgagtgatg tccgcagcgc cgtgggtaat
    16681 tctcccgaac tgcgcagacg ctgcaagatc acaggttcaa ggcttagcca tgagaaaacg
    16741 cctgcgccta aaaacaccag accggcgtcg gtgtagccca acgcaccaca ggccattgcg
    16801 ctgataaagt tgttggcaac tgtcggcaga tacagtccag gcgtggtagc ttcttcaggg
    16861 tgagatccgc gccataatcc cgcagtttgc caggcggcat aagccaactg aaccacgacg
    16921 ccaaaactga acaggcacac cgccagtggg cgaaaccacg gaacaaaacc aatcgccacc
    16981 agcatcgtcg ttgccggaaa caaactcaca aaactgctca gcactggatg gcgcacttcc
    17041 gccagcacgc tatgcggaaa gcgtatgagt cgggtaataa atgcgctagt taataatccc
    17101 cagatgatca tcgccagaat caccagccca tcccctaacc agtggctgac ctgccaaacc
    17161 tggctggcat agcgccaggc aaatcccatc cctatcgtcc ccaacacaat accaaagtag
    17221 cctgccggca aattgagcac tttatcgctc tgcatcttct ta
//
LOCUS       DAZGQL010000103        17214 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.103, whole genome shotgun sequence.
ACCESSION   DAZGQL010000103 DAZGQL010000000
VERSION     DAZGQL010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17214
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.103"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            157..411
                     /gene="bssS"
                     /locus_tag="ACHKIR_004051"
     CDS             157..411
                     /gene="bssS"
                     /locus_tag="ACHKIR_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HFO9991727.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            526..1644
                     /gene="solA"
                     /locus_tag="ACHKIR_004052"
     CDS             526..1644
                     /gene="solA"
                     /locus_tag="ACHKIR_004052"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HFO9991728.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEEDPIFVRSGVINLGPADSTFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETADGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
     gene            1692..1805
                     /locus_tag="ACHKIR_004053"
     CDS             1692..1805
                     /locus_tag="ACHKIR_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HFO9991729.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVYYF"
     gene            2066..2632
                     /gene="yceJ"
                     /locus_tag="ACHKIR_004054"
     CDS             2066..2632
                     /gene="yceJ"
                     /locus_tag="ACHKIR_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFO9991730.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHQAPELHKSIGILLMMGLVIRVLWRVISPPPGPLQSYSPMTRLGAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            2636..3211
                     /gene="yceI"
                     /locus_tag="ACHKIR_004055"
     CDS             2636..3211
                     /gene="yceI"
                     /locus_tag="ACHKIR_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HFO9991731.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(3253..4305)
                     /gene="trhO"
                     /locus_tag="ACHKIR_004056"
     CDS             complement(3253..4305)
                     /gene="trhO"
                     /locus_tag="ACHKIR_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFO9991732.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALDVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            4530..5450
                     /gene="lpxL"
                     /locus_tag="ACHKIR_004057"
     CDS             4530..5450
                     /gene="lpxL"
                     /locus_tag="ACHKIR_004057"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HFO9991733.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKTLKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            5622..6848
                     /gene="mdtG"
                     /locus_tag="ACHKIR_004058"
     CDS             5622..6848
                     /gene="mdtG"
                     /locus_tag="ACHKIR_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
                     /protein_id="HFO9991734.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSITFLFSAIASPFWGGLADRKGRKLMLLRSALGMGIVMVLMG
                     LAQNIWQFLILRALLGLLGGFVPNANALIATQVPRNKSGWALGTLSTGGVSGALLGPM
                     AGGLLADSYGLRPVFFITASVLILCFFVTLFCIREKFQPVSKKEMLHMREVVTSLKNP
                     KLVLSLFVTTLIIQVATGSIAPILTLYVRELAGNVSNVAFISGMIASVPGVAALLSAP
                     RLGKLGDRIGPEKILITALIFSVLLLIPMSYVQTPLQLGILRFLLGAADGALLPAVQT
                     LLVYNSSNQIAGRIFSYNQSFRDIGNVTGPLMGAAISANYGFRAVFLVTAGVVLFNAV
                     YSWNSLRRRRIPQVSN"
     gene            6931..7305
                     /gene="msyB"
                     /locus_tag="ACHKIR_004059"
     CDS             6931..7305
                     /gene="msyB"
                     /locus_tag="ACHKIR_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFO9991735.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGRAAADEWDER"
     gene            complement(7306..7533)
                     /gene="yceK"
                     /locus_tag="ACHKIR_004060"
     CDS             complement(7306..7533)
                     /gene="yceK"
                     /locus_tag="ACHKIR_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFO9991736.1"
                     /translation="MRLIVMSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDIHHGPYE"
     gene            complement(7705..10248)
                     /gene="mdoH"
                     /locus_tag="ACHKIR_004061"
     CDS             complement(7705..10248)
                     /gene="mdoH"
                     /locus_tag="ACHKIR_004061"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HFO9991737.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDVWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(10241..11776)
                     /gene="mdoG"
                     /locus_tag="ACHKIR_004062"
     CDS             complement(10241..11776)
                     /gene="mdoG"
                     /locus_tag="ACHKIR_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HFO9991738.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            12170..13327
                     /gene="mdoC"
                     /locus_tag="ACHKIR_004063"
     CDS             12170..13327
                     /gene="mdoC"
                     /locus_tag="ACHKIR_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HFO9991739.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(13335..14816)
                     /gene="clsC"
                     /locus_tag="ACHKIR_004064"
     CDS             complement(13335..14816)
                     /gene="clsC"
                     /locus_tag="ACHKIR_004064"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HFO9991740.1"
                     /translation="MMKKTPTSTKDSLLNKEMNDLPRLASAVLPLCSQHPGQCGIFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(14758..15291)
                     /gene="ymdB"
                     /locus_tag="ACHKIR_004065"
     CDS             complement(14758..15291)
                     /gene="ymdB"
                     /locus_tag="ACHKIR_004065"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HFO9991741.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(15386..15697)
                     /gene="ymdA"
                     /locus_tag="ACHKIR_004066"
     CDS             complement(15386..15697)
                     /gene="ymdA"
                     /locus_tag="ACHKIR_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmdA"
                     /protein_id="HFO9991742.1"
                     /translation="MFRPFLDSLMLGSMFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPGRTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(15818..16150)
                     /gene="csgC"
                     /locus_tag="ACHKIR_004067"
     CDS             complement(15818..16150)
                     /gene="csgC"
                     /locus_tag="ACHKIR_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly chaperone CsgC"
                     /protein_id="HFO9991743.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDMYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPDDRVKIVVTVSDGQSLHLSQQ
                     WPPSSEKS"
     gene            complement(16209..16664)
                     /gene="csgA"
                     /locus_tag="ACHKIR_004068"
     CDS             complement(16209..16664)
                     /gene="csgA"
                     /locus_tag="ACHKIR_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli major subunit CsgA"
                     /protein_id="HFO9991744.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGGGNNSGPNSE
                     LNIYQYGGGNSALALQADARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSAT
                     LDQWNGKDSTMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
     gene            complement(16705..17160)
                     /gene="csgB"
                     /locus_tag="ACHKIR_004069"
     CDS             complement(16705..17160)
                     /gene="csgB"
                     /locus_tag="ACHKIR_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli minor subunit CsgB"
                     /protein_id="HFO9991745.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
BASE COUNT         4312 a         4385 c         4089 g         4428 t
ORIGIN      
        1 tattgttcat ataaatctta aaagcaaaaa tttatctgtt ccccccttta aaaattcggt
       61 gaataccctt acttattggt atactgaagt tgcttcagat aaaaacgcat tgaacctcga
      121 ataacgttgt ctagtaacac gaattagggg gccatgatgg aaaaaaataa tgaagtcatt
      181 cagactcatc cgctcgtagg gtgggacatc agcaccgttg atagctatga tgcgctgatg
      241 ttgcgtttgc actaccagac cccaaataag tccgagcagg aagggactga agttggtcag
      301 acgctctggt taaccactga tgttgccaga caatttattt cgatattaga agcaggaatc
      361 gccaaaattg aatccggtga tttcccggta aacgagtatc ggcgtcatta acatgacatg
      421 accatccacc caaaaggcac ctcaccggtg cctttaccat ttctgggttg tatatctgca
      481 ttcagttaat tactctgctt aaaccatgag agcagagaga aaaaaatgaa atacgatctc
      541 atcattattg gcagcggttc cgtaggcgct gccgccgggt attatgcaac ccgtgccggt
      601 ttaaacgtgc taatgaccga cgcccatatg ccaccgcatc aacacggcag ccaccacggc
      661 gatacgcgat taattcgcca tgcttatggt gaaggcgaaa agtatgtccc gctggttctc
      721 cgcgcgcaaa cgctgtggga tgaactctcc cgccacaacg aagaagatcc catttttgta
      781 cgctctggcg tcattaacct tggcccggct gattccacat ttctcgccaa cgtcgcccac
      841 agcgccgaac aatggcaact caacgttgaa aagctcgatg cgcaagggat tatggcccgc
      901 tggccagaaa tacgcgtccc ggacaactac atcggcttat ttgagactga ttccggcttt
      961 ttacgcagcg aactggcgat taaaacctgg atccaactgg cgaaggaagc gggctgtgcg
     1021 cagctgttca actgcccggt caccgcaatt cgtcatgacg atgatggcgt aactattgaa
     1081 acggctgacg gtgagtatca ggcgaaaaaa gcgattgtct gcgcgggaac atgggtaaaa
     1141 gacctgctcc cggagttgcc tgtccagccc gtacgcaaag tatttgcctg gtatcaggcc
     1201 gatgggcgct atagcgtgaa gaataaattc ccggcgttta ccggtgaact tcccaatggc
     1261 gatcaatatt atggttttcc ggcagaaaac gacgcgttga agattggcaa acataacgga
     1321 ggccaggtta tccattcagc ggatgaacgt gttccgtttg cggaagtggt cagcgatggt
     1381 tcggaagcct tcccgttctt gcgcaatgta ttgccgggta tcggttgctg cctgtacggc
     1441 gccgcctgca cctatgataa ttcgcctgac gaagatttta ttatcgatac cctacccggc
     1501 cacgataata cactgctcat taccggcctg agtgggcacg gttttaaatt tgcgtcagtt
     1561 ttaggggaaa tagctgccga ttttgcgcaa gacaaaaaaa gcgattttga tttgacgcca
     1621 ttcaggcttt cccgcttcca ataatcatca ttacggcctc cagaatgagg ccgtttttac
     1681 aggagtattt aatgcgtcgg ctgttgcact atctcatcaa taatattcgc gagcatctga
     1741 tgctttatct ttttctctgg ggattgttgg caattatgga tctgatctat gtgtattatt
     1801 tttagttttc cgcactgaca taaactgaca aatatttaat ctccctttca tttagcgtga
     1861 aaaattgaat gagattattc ctgcttcaac agcactcacc agacatctct gatgctgaac
     1921 ccactaaact tatgaagatt ctttcattat taattcaaaa ttattgaaat aaaaataaat
     1981 atcattcaaa ttgttatttt tgtgttgcaa ttcattctcc gacacgccat tttctttctc
     2041 tcgcaaagtc gttttggatg cacttatgtc attcacaaat acccctgaac gctatggtgt
     2101 tatatctgcg gccttccatt ggctaagcgc aattattgtc tacggcatgt ttgctttagg
     2161 tctgtggatg gtcacgctca gttattacga tggctggtat caccaggcac ccgaactgca
     2221 taaaagcatc ggtattttgt taatgatggg gctggttatt cgcgttctgt ggcgcgtcat
     2281 atctccaccg ccgggtccgc tgcagagtta ttcgcccatg actcgtcttg gcgcaaaggc
     2341 tggacatctc gcattgtact tattgctgtt cgccatcggc atcagcggct atctgatctc
     2401 aactgccgat ggtaaaccga tcagcgtttt tggctggttt gacgtccccg cgaccctcgc
     2461 cgacgctggc gcacaggctg actttgccgg tgccctgcat ttttggctcg cgtggagcgt
     2521 cgtagtactg tccgttatgc acggatttat ggccctgaag catcatttca tcgataaaga
     2581 cgacactctg aagcgcatgc tgggaaagtc gtcatctgac tatggagtat aaaaaatgaa
     2641 aaaaagcctg cttggtttaa ccttcgcttc cctgatgttc tctgccggtt cagcggttgc
     2701 cgccgattac aaaattgaca aagaaggtca gcacgccttt gttaacttcc gcatacagca
     2761 ccttggctat agctggttat acggcacctt taaagatttc gacggtactt ttacctttga
     2821 tgaaaaaaat ccagctgccg ataaagtgaa tgtgacaatt aacaccacca gcgtcgatac
     2881 taatcacgcc gaacgcgata aacatcttcg cagtgcagat ttcctcaata ctgcaaaata
     2941 tccacaggca acattcacct ccaccagcgt gaagaaagac ggtgacgaac tggatattac
     3001 cggcgatctg acgctgaatg gcgtaaccaa acccgtcacg ctggaagcga aattaattgg
     3061 tcagggcgac gacccatggg gtggtaaacg tgcaggcttc gaggccgaag gcaaaattaa
     3121 gctcaaggac ttcaatatca agacagattt aggtccagct tctcaggaag tggatctgat
     3181 tatttcagtg gaaggcgtac agcagaagta agagtgaaat gttgccggat ggcacgcatc
     3241 cggcaatgat atttattctg ttggatcagg aatgcccagt gttgtattca gacgtccacg
     3301 agacttatta aagatcttat tgccattttc acgtcctgcc cgacggcgtc gctgttcttc
     3361 tggcggtaac gcgctttctt cgcagcaaat ttcactacaa caacctttgt atttttctgc
     3421 acatactgga cactgaataa acagcaggtg gcagccatca tttttacagt tggtatggct
     3481 gtcgcacggc gcaccgcact ggtggcaatg cgcgataatc tcatcagata tacgttcgcc
     3541 catccgctcg tcaaaaacaa aatttttgcc aataaaacgt accggtaaac cctgctcgcg
     3601 cgccttgcgg gcgtattcaa taattccgcc ctcgatatgc cagactttat tgaatccgtt
     3661 atgtttcatc caggcactgg ccttttcaca acgaatgccg ccggtgcagt acataacgat
     3721 ttttttatct ttatgtgcct gcatcatctc tactgctttt ggcagctgct cacggaaggt
     3781 atctgccgga atttccagcg cgttttcaaa gtgccccact tcatactcat agtggttacg
     3841 catgtcgata aacaatgcat cgggatcgtc aagcatggcg ttcacttccg ccgcttgcag
     3901 atactcaccc acattgctgg catcaaagtg aggatcgtca ataccgtcgg caacgatacg
     3961 atcgcggacc ttcatgcgca gtacccagaa ggatttcccg tcatcatcca acgcgatatt
     4021 caggcgtaag ccctctaaag ccgggtcgaa ggcataaagc tgcgcgcgaa atgtttcaac
     4081 attgctcgca ggtacgctga tttgcgcgtt aatgccctca tgcgccagat acactcgccc
     4141 aaaaacatcc agcgcggtaa acagctgata taaagcgtca cgggtcgcct taggatcggc
     4201 gatgtggaaa tacttgtaaa acgaaatggt ggttcgcggt tcgctctcag ccaacatttt
     4261 ggcttttaac gcgtcgttgg aaatgcggtt gtgtaacact ggcatggtgt acggttcctg
     4321 cgagatggga aagtaaaaat ccgcggcatg atatagcaat tatcgataat taacatccac
     4381 acattttacg ctacatttgc gcattaaaaa ttatttgtta tttacaagcg cggcaatttc
     4441 gcccagtctt cagccacaaa ttttggttgc gggcgaaaaa atgcgacaat acatacaatt
     4501 gcccgtatag gttgaaaaac aggattgata tgacgaatct acccaagttc tccaccgcac
     4561 tgcttcatcc gcgttattgg ttaacctggt tgggtattgg cgtactttgg ttagtcgtgc
     4621 aattgcccta cccggttatc taccgcctcg gttgtggatt aggaaaactt gcgttacgtt
     4681 ttatgaaacg acgcgcaaaa attgtgcatc gcaacctgga actgtgcttc ccggaaatga
     4741 gcgaacaaga acgccgtaaa atggtggtga agaatttcga atccgttggc atgggcctga
     4801 tggaaaccgg catggcgtgg ttctggccgg accgcagaat cgcccgctgg acggaagtga
     4861 tcggcatgga acacattcgt gacgtgcagg cgcaaaaacg cggcatcctg ttagttggca
     4921 tccattttct gacactggag cttggtgcgc ggcagtttgg tatgcaggaa ccggggattg
     4981 gcgtttaccg cccgaacgat aatccactga ttgactggct acaaacctgg ggccgtttgc
     5041 gctcaaataa atcgatgctc gaccgcaaag atttaaaagg catgattaaa accctgaaaa
     5101 aaggcgaagt ggtctggtac gcaccggatc atgattacgg cccgcgctca agcgtttttg
     5161 tcccgttgtt tgccgttgag caggctgcga ccacgaccgg aacctggatg ctggcacgga
     5221 tgtccggcgc atgtctggtg cccttcgttc cacgccgtaa gccagatggc aaagggtatc
     5281 agttgattat gctgccgcca gagtgttctc cgccgctgga tgatgccgaa actaccgccg
     5341 cgtggatgaa caaagtggtc gaaaaatgca tcatgatggc accagagcag tatatgtggt
     5401 tacaccgtcg ctttaaaaca cgtccggaag gcgttccttc acgctattaa atctcccaag
     5461 ccggatgctt gagaatagca tccggcatta ccacagcaaa tccccctgat ttagcgataa
     5521 aagctctctg gattgcgccc cctggaagtc gggcgcataa ttagtgtgct tatcttttct
     5581 tcttatgttc accgcgcctg gcgcaccaac agcggattgc tatgtcaccc tgtgaaaatg
     5641 acacccctat aaactggaaa cgaaacctga tcgtcgcctg gctaggctgt tttcttacgg
     5701 gtgccgcctt cagcctggta atgcccttct tacccctcta cgttgagcag cttggcgtta
     5761 cgggccactc cgccttgaat atgtggtccg gtattgtctt cagcattaca tttttatttt
     5821 cggccatcgc ctcaccgttt tggggtggac tcgccgaccg taaaggccga aaactcatgc
     5881 tattacgctc tgccctcggc atgggcatcg tgatggtgtt gatgggactg gcacaaaata
     5941 tctggcagtt tttgatcctg cgggcgcttc ttgggttact tggcggattt gtccccaacg
     6001 ctaatgctct tatcgccaca caagtaccgc gtaataaaag cggctgggcg ctgggtacgc
     6061 tctccacagg cggcgttagt ggtgcgttgc tcggcccaat ggctggcggc ctgctcgccg
     6121 atagctacgg cttacgtccg gtattcttta ttaccgccag tgtgctcata ctctgctttt
     6181 tcgtcaccct gttctgcatc agagaaaaat tccagccggt cagcaaaaaa gagatgctgc
     6241 acatgcggga agtggtgaca tcacttaaaa acccgaaact ggtactcagc ctgtttgtca
     6301 ccacgttaat catccaggtg gcgacaggct caattgcccc cattctgacg ctgtatgtcc
     6361 gcgaactggc gggtaacgtc agtaacgtcg cctttatcag tggcatgatc gcctcggtgc
     6421 caggcgtggc ggctctgcta agcgcaccac gactcggcaa acttggcgat cgaatcggac
     6481 ccgaaaagat cctgattaca gcgctgatct tttctgtact gctgttgatc ccaatgtctt
     6541 acgttcagac gccattgcaa cttgggattt tacgtttttt gctcggtgcc gccgatggtg
     6601 cactactccc cgccgtacag acactgttgg tttacaactc gagcaaccag atcgccgggc
     6661 gtatcttcag ctataaccaa tcgtttcgtg atattggcaa cgttaccgga ccattgatgg
     6721 gagcagcgat ttcagcgaac tacggtttca gagcggtatt tctcgtcacc gctggcgtcg
     6781 tgttattcaa cgcagtctat tcatggaaca gtctacgtcg tcgtcgaata ccccaggtat
     6841 cgaactgatt tttcgccttt catacttgca aaagcggaga atcagctatc cttttccctg
     6901 aaacctcatc aactcaaagg gagaatcgtg atgaccatgt acgcaacgct tgaagaagcc
     6961 attgacgctg cacgcgaaga atttcttgca gacaaccccg gcatcgacgc cgaagatgcg
     7021 aatgtgcaac agttcaatgc ccaaaaatac gttttgcagg acggcgacat catgtggcaa
     7081 gttgagtttt ttgccgacga aggggaagaa ggtgaatgtt tacctatgct tagcggtgaa
     7141 gccgcgcaaa gtgtttttga tggcgactat gatgagatag agatacgcca ggagtggcag
     7201 gaagagaata cattacatga atgggacgag ggggaatttc agcttgagcc accgctggat
     7261 accgaggaag gacgcgcagc agctgatgag tgggatgaac gttaatcact catacgggcc
     7321 atgatgaatg tcgatcggca gcagtaaagt atcgaaaacc agggagaatg gcagatcaag
     7381 gatcgtgacg taacgccagg cggagtcacg cacgtcccat tgcacaccgg gataatattg
     7441 gttgccatgc ccctgccccg gtatagtgcg actaatgatg ctgccacagc cactcagcaa
     7501 ggtcaccatt atgctcatca caattaaacg catttgatac ctcttccgta aaaaataccg
     7561 gcataacgcc ggtattttta ttggctcgtt gtttttgccg gatgcggcgt gaacgcttta
     7621 cccgacctac aaaatcttgc aaaatcaata aattgcagga acgatgtagg cctgataagc
     7681 gtagcgcatc aggcaactac gtttttattg cgaagccgca tccggtttac gcaatgccag
     7741 tggattgagc tttatccctt cgtaataact cacccatgaa gaatatctct ccggggaatt
     7801 ccagacgcgg aaatgcagac gggccatcgt caccggatcg cttagcagca ccaggcgacg
     7861 atcgcgattc agcttctctg gcgtctcgtt cagcgcctgt tcaacgtggc ggtcacgggc
     7921 gatttccagc accttgctgg cgcggtgacg cgcggtcgcc attgcggttg ccagagcgtt
     7981 aaatgacggg ttaaacaccg cgtgcataaa accatcatca agggagcgtt gacgattcat
     8041 ctcaaggaac cgatcggtat caaccagcac ctgcggcggt gaatactctt ccgggatcag
     8101 gaacagtttc cagcgtttgg ttcgcagacc aacggtggca cggctggaaa tcaccgaaac
     8161 aaacggtgac aggatcaacg agaagacaat cggtgccagc cagaacagaa aacgcagatc
     8221 caaccacgcc atcccaacag cccacactaa acccaacagc agctgtgagc cgtggcgttt
     8281 gaatgcttcg ccccaggaag tggagtcatc atcacgctgc ggtgaattcc acaccacttc
     8341 ccagccaagg aacgcgctga cgacgaagac tgtatggaac aacatgcgta ccggagccag
     8401 caacacggaa aacagcactt ccagcagcaa cgataatgta acgcgccaga agccgccgta
     8461 ttctttcgtt cctttgcacc agataagcaa aatgctcaat agcttcggca ggaacaacag
     8521 caccatggtc gaagcaaaaa gtgcaatcgc cagctcagga cgccactgcg gccataccgg
     8581 gaacaactgc cgtggttgca ggaagtattg cggttcggtc aacgcatgta ctacctgcaa
     8641 tgcagtagag agcgcgagga acataaacca cagcggagcg gagagataag acatcacgcc
     8701 cgtcaggaac accgcacggt gaaccgggtg catacccttc accaggaaca gacggaagtt
     8761 catcaggtta ccgtggcacc agcggcggtc acgttttagc tcatcaagca agttaggcgg
     8821 caattcttca taagaacccg ggagatcgta agcaatccac accccccaac ctgcacggcg
     8881 catcaacgcc gcttctacga agtcatgtga caggattgaa ccggcaaaag aaccttcgcc
     8941 cggcagcgga gccagtgcac aatgctcgat aaacggtttc acgcggataa tcgcgttatg
     9001 cccccagtag tgcgactcgc caagttgcca gaagtgcaaa ccggctgtaa acagtggccc
     9061 atacacgcgg gtcgcgaact gctgacagcg cgcatacagc gtatccatac cggacgcttt
     9121 cggcgacgac tgaatgatcc cggcgttcgg gttggcttcc atcaagcgca ccaggccgca
     9181 taaacaatca ccggtcatta ccgagtcagc atccagcacc accatgtagc tgtactggct
     9241 gccccagcga cggcagaagt catcgatatt accgctttta cgcttcacgc gacggcggcg
     9301 gcggcgatag aaaatctgac cttcgccacc gacttcagcg ataagctcca tccaggcttt
     9361 ttgctctgcg acgcagatat ccgggttata actgtcactc agaatgtaga catcaaagtg
     9421 tttggcattc ccggtggctt ttactgattc ccacgttgca cgcaagccag caaaaacacg
     9481 gttcacgtct tcgttacaga taggcatgat caacgccgtg cgatgctccg ggtttaatgg
     9541 ttcatcgcca actgttgacg cggatatact gtatttatcg cgaccaataa gcagttgcag
     9601 gaagcccatc aacgccgtcc agaatccggc ggacacccaa cagaacagta ccgcaaagag
     9661 gatcaggata ccggtttgca gcatataagg cagaagctgc ataaaagaaa cccacacatc
     9721 ctgaccaacc atatccatag gattaatcag cgcccacccc tgataaggaa gaatggtctt
     9781 catataccag gtcgcgacaa cagtttgcgc gagcgtcagg atcaacagaa tgtaacggcg
     9841 gatggtaccg acggtacgcc acttttgctc gctctcctgc tcttctttgg tcaaacgagc
     9901 cagatagcgc ggcgtcacat cgcgtccacg caggcgatcc cagaaacggc ctaccgggtt
     9961 ggtacgccac gggtcgggaa acatcgagga gcgttttgct tctggcatcg ccttaagctg
    10021 atcgcgccct tcgtcatctt taattaactg tccatcagca agtgaatctg gccaggcttg
    10081 ttccagacgc gcctttaccg agccttgcgg ggagtcatcc tcccgcgccc aggtgcggtg
    10141 ttcggcatcc agcgcctgat gaacggcgcg gatatcagtc ttcggcaatg ccgctttctc
    10201 gcttgcggcg atgggcattg cgtcaatgta ctcagttgtc ttattcattg gcaggtaact
    10261 ggtagctcca ggtttcactc aacgtctgat cggcattcac cagcgcagca cgcatttcag
    10321 tggttttctt ggcatctttc actttcacac gcatcaccag acgccagcct ttggtaaccg
    10381 ggttatagcg caccgtgctt tcaactatct caccattatc accaatgctg gtttgcgctg
    10441 tgaccggggt atcctctggc agttttttca tctctgcgcc ggtaaaatcg accacaaagg
    10501 cgatagtacc gtcaggttgg cgaatcaggt tcgactgctt cacatccccc gttgaacgac
    10561 gcgtttgttg cacccatgcg ttatctggcg catgcagttt gtcttcatca cggctgaagg
    10621 tgatggtgta tttaaagttc atctctttac ccggctccgg cagctgatcc ggcgtccagt
    10681 aagcgacgat gttatcgttg gtttcatcgt tggttggaat ttccaccagc tcaacgctgc
    10741 ctttgcccca ctcccctttc ggagtcaccc atgcgcttgg acggagatcg taacgatcat
    10801 cgagatcttc aaagcgggag aaatcacgac cgcgctgcaa cagaccgaag ccttgcgggt
    10861 tttccatcga gaagctgctg accgctaaat gtttcgggtt attcaacgga cgccagatcc
    10921 attcgccatt accagcatgg atagagagac cgttagagtc gtgcaactcc ggacgatagt
    10981 tatttgcagg cgacggttgg ttcggcccaa acaggaacat actggttaac ggtgcaaccc
    11041 ccagtttgcc gactttatcg cgcagataga ttttcgactg cacatccaca accgtgtcac
    11101 gccctggcat aactacgaat ttgtaagcac ctgtcgcgcg cggcgagtca agcaatgcat
    11161 aaatggttaa acgtttatca gtcggttttg gacgctcgat ccagaactct ttgaagcgtg
    11221 gaaattcttc acccgatggc aaggcggtat caattgccag gccgcgtgca gaaaggccat
    11281 aaacctgacc tgcaccaatc acgcggaaat agctggcccc gagcatgctg acgatttcat
    11341 cgtttttatc tttgctgttg atcgggtaaa gcactttaaa accggcaaaa ccaaggtctt
    11401 ttaccgtgtc tttgtcatgc tgaacatcgc cgaaagtgaa ataatccggg ctgtatttga
    11461 ttcgtttgac tgcggtggca gtcacttcat ttattttgac cggggtatcg aagtacatac
    11521 cctgatggta gaactcgagt ttgaatgggg tcttcagatt gttccagtac gctttgtcat
    11581 gattaaactg gatctgctga tagtccgcgt atttcatgtc gcggaaaacg gagggcaagt
    11641 tgcttttagg cgcctcatag cctttcccgg ctaaggattg agcttgcttt gcgacatcat
    11701 caatactgaa agcccagctt gaagatgtat acagggttaa cattactgca gcactcaacc
    11761 aacgcatttt catcatttgt agtttatgtt tcataattag taagcacttc cccctttgtg
    11821 tgcttatatc gatccgatct attttaatgg ataaccagcc attccgacaa cagaatcccg
    11881 gtatcgttca gcacgctggc tcatggatta agcacctgat tgcaaccgtt ttcactttcc
    11941 gtgcttctcc taaagacaga agcatcgact tcgtgtaggg tttgccagca aatttattta
    12001 tcaaccgtct tacagacgct tccattctgg ttagtggtag cgatgatgct atgcataaaa
    12061 accttgaagc ctgcgcctgg cgcggtcagg tcggtgtcgc tatggcaaca agcctaaagg
    12121 cataacccga cagggataag acgaaaaatc cgagattagt taaccatata tgaatccagt
    12181 acccgcgcaa cgtgaatatt tcctcgactc catccgcgcc tggctgatgt tgttagggat
    12241 cccttttcat atttctttaa tctattcgag ccatacatgg catgtgaata gcgccgaacc
    12301 gtcattgtgg ctgacccttt ttaatgactt catccactcg ttccgcatgc aggtattttt
    12361 cgttatatcc gggtactttt cctacatgct ttttttacgc tatcccttga aaaaatggtg
    12421 gaaagtacgt gtcgaacgtg taggtatccc gatgttaaca gccatccccc tactgacatt
    12481 accgcaattt attatgctgc aatacgtcaa agggaaagcg gaaagttggc ctgggctatc
    12541 attgtatgac aaatataata cgttggcctg ggaattaata tcacacctgt ggtttttact
    12601 ggtgttagta gtcatgacga cgctgtgcgt atggatattt aaacgcatca gaaataattt
    12661 agaaaattct gataaaacga ataaaaaatt ctcgatggta aaactatcgg tgattttttt
    12721 gtgcctcggc atcggttatg cggtaataag aagaacgatt tttattgtgt atccgcccat
    12781 tctgagtaat ggcatgttca attttattgt catgcaaacg ctattttatt tgccgttctt
    12841 tatcctcggc gcactggctt tcattttccc tcatcttaaa gccttgttta ccacgccgtc
    12901 tcgtggctgt acccttgcag cagcattggc atttgtcgct tatttactca accagcgcta
    12961 tggcagtggc gatgcctgga tgtacgaaac cgagtcggtg atcaccatgg tcctcggtct
    13021 gtggatggtg aatgtggtct tctcctttgg ccaccgtttg cttaacttcc agtcagcgcg
    13081 ggtgacttat tttgttaacg catcgctgtt tatctatctg gttcaccacc cgttaacgct
    13141 gtttttcggc gcatacatta caccgcacat cacctccaac tggcttggtt ttctctgtgg
    13201 cctgatattc gtagtaggga ttgcgataat tctgtatgaa attcatttac gcatcccgtt
    13261 actgaagttt ttgttctctg gtaaaccggt tgtcaagcgt gagaacgata aagcaccagc
    13321 ccgttaagcc acgcttataa taaccattcc acgggcaata tcgacgccag tctgaccata
    13381 acccgcttcc agaaactggt ggcgggttct tttttgagaa taatctcttt ctttgcatga
    13441 cgatcaaccc agttgatccg tccccacctg tccagacgga gctgccaggc cgcatcatac
    13501 tggctctgaa taaagcgttt atcaattaac tgtgccagcg tttcgctctc tatcacgaag
    13561 cccatttcag tattgagcaa tgttgaacgc ggatcgaaat tgaaagagcc gataaacacc
    13621 gttttaccat cgatgctaaa ggttttagca tgcaagctgg caccggaatt accggttatg
    13681 ccgcgatcgt gtaacgtact actttgttca cgcgtcggct tgagttcata taattccacg
    13741 ccatagcgga gcaatttttt gcgccagcgc gcgtatccgg catggacgac agcaacatcg
    13801 ttagcggcaa gagaattggt taggatcgca atctttaccc cttttctcac catccgtaag
    13861 agttgcgcca cacctgcgcg tgtcggtaca aaataggaag agataatatc gatgcgttca
    13921 ctgggtgagc ccatgatatc gaacaggcgc tgcggtaaca gtgaatgccg ttttgccttg
    13981 ccttcccctt tcgccggatc atcacttaat aaacgtgttt tcgcccagat aagcggcaac
    14041 gttccatcaa ccagatgatt tataaacggg ctggattcca ttttgcgtaa ataacgatgc
    14101 gtcatggcat cgttgtgcca ggatgctggt aactcgatgc gatccgccat ttcaccttcc
    14161 gggacatcca gcacctgctg taggggtgaa accgatttgc aataccagta gcgggcgaaa
    14221 tcatcggcaa cgtcctctac aactggtcct attgccatga catctaaatc cgaaaaaagt
    14281 ggctcttccc ctgccccaaa ataggcatca ccaatatttc gcccccctac cagcgtcacc
    14341 acgccatcga cagtgaaact tttattgtgc atacggcgat tgagacggga aaagtcggtg
    14401 atataaccaa gcggacgtag caagcgaaac gagaaaggat taaaaagccg gacttcaatg
    14461 cggggatgac tgtcaagcaa gcgtaaaatg tcgtcaagtc cgggcgtatt gttgtcgtcc
    14521 agcaacaaac ggacgcgaac gccacgcttt gctgcggcta acagggcgga aaagagtaac
    14581 cgacccgaca tatcgtcctg ccagatgtaa tactgaacat ccagcgtatg ctctgccatt
    14641 tcggccagac gataccgggc ggcaaacgca tccagacttt tctccagagg aaaaatgcca
    14701 cactgaccgg gatgttgcga acacagtggc agcaccgcac tcgccagccg gggcaaatca
    14761 ttcatctcct tgttgagtaa ggagtctttc gtagaggtgg gcgttttctt catcataaca
    14821 gacaaagtat acctgttcgg gtaaagcgtg acgggtaata aattctgaaa cggtttttac
    14881 tgcgatttca gccgctgccg cacgagggta accataaact ccagtactga ttgcaggaaa
    14941 agccactgac gtatagctgt ttgctgccac cagtcgcagg ctattgagat aggcatcctg
    15001 caaaagctgg tcttcgtttt gttcaccacc acgccagact ggcccgacgg tgtgcactac
    15061 ggctttcgcg ggaagatcgc ctgcaagcgt aataacggca tgccccgtag ggcaatcgcc
    15121 ctgctgctgc ctgactttta aacaggcatc cagcagggcc ggacccgctg cgcgatgaat
    15181 ggccccatcg acgccgccgc ctcccattaa tgacggatta gccgcattca caatcacatc
    15241 aacggccagt ttggtaatat caccctgcac aacatgaata cgcgttttca taacgcctcc
    15301 ttacaccttc ttgtttctta agtgtacagc aggctttttg gttaaatcgc atttttctgc
    15361 tcagcaacgt gagttgttca gaaacttagt tatattcgat gttcattaca gccagctttt
    15421 tctgtttatc aagatactgc acttttactg aggctatttg cgcgtttttg acattgccgc
    15481 tcattaacgc tttgctggaa taggttctgc ccggaacaga gccattctgt gggcagttca
    15541 tttctacggg tgatgactgg gtgctgacgt cacatgccgg ttccacaatt tggccataaa
    15601 aatggatcac gcccccttgc gcggtgcttc ctgcgatggc aataaaagga aaaaacatac
    15661 tgccgagcat tagagagtct aaaaaaggtc tgaacattta ttctatttcc tcaatgatta
    15721 gtcatccttg agggttgtgt tatccatact ttcaggttgg ctgatccgca gcagaccatt
    15781 ctctccagat tcatcttatg ctcgatattt caacaaatta agacttttct gaagagggcg
    15841 gccattgttg tgataaatga agtgactgtc catcagaaac agtaacaact attttcaccc
    15901 gatcgtccgg ggaaatattt aaactcaact tcgtcaaagc aatgggttga ttagcaggca
    15961 atgagagggt cttttcttgc ttcgtctgac tttgccctga actgccttcg cgcagggaca
    16021 atatttgtac tctgcacaga caagattgag taagagtgac ttcaggaata atggtgtaca
    16081 tatccccttg ctgggtcgta ttaaaggtta tctgactgga aagtgccgca aggagtaata
    16141 acgcattcat attcttctcc cgaaaaaaaa cagggcttgc gccctgtttc tttaatacag
    16201 aggatgtatt agtactgatg agcggtcgcg ttgttaccaa agccaacctg agtgacgttg
    16261 acgctggagt tagatgcagt ctggtcaaca gcagcaccgt taccaccacc gaactgttta
    16321 acagtcatag tagaatcttt accattccac tgatcaagag tagcgctgtt gccgaaacca
    16381 cgctgagtca gatcgattga gctgtcatca gaaccttggc caacatctgc gccgttaccg
    16441 ccgccgtgct gggtaatggt cagatcagag ttacgggcgt cagcttgcag agcaagagca
    16501 gagttaccgc caccgtactg gtaaatgttc agctctgaat ttgggccgct attattaccg
    16561 ccaccaccgt ggttaccgcc gccgccgtac tgaggaacaa cacctgccag agcgctacca
    16621 gagaatacga ttgctgcaat tgctgctact tttaaaagtt tcatgtaaaa cccccatcgg
    16681 attgatttaa aagtcgaatg gaaattaacg ttgtgtcacg cgaatagcca tttgcgactg
    16741 tctctgcact acaattgccg ttttttgagt accatactgt gtaatatttg ctttattacc
    16801 agaacctttc tggataatca tcgcagtatt accataagca ccttgcgaaa tactggcatc
    16861 gttggcactg cccgcctgat caatatatgc aaggttataa tctcctgtct ggtcaatctt
    16921 tgcccggttg ctactacctt cttgcgcaac aaccgccaaa agttttgagc ctccctgccg
    16981 taactgagca ctattattag tcccagcttg accaattatg gctgcctgat taaatgaaga
    17041 cttactcaat tcatttaccg cgaagttata ttctgaatta gctaaatcat aacctgctgc
    17101 ggctgcaatc ccaggcgcac ccagtattgt taacatcata aataacaatt tgtttttcat
    17161 gttgtcaccc tggacctggt cgtacattta agaaattaaa tcatttcaac ttgg
//
LOCUS       DAZGQL010000104        17194 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.104, whole genome shotgun sequence.
ACCESSION   DAZGQL010000104 DAZGQL010000000
VERSION     DAZGQL010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17194
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.104"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..288)
                     /locus_tag="ACHKIR_004070"
     CDS             complement(<1..288)
                     /locus_tag="ACHKIR_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO9991746.1"
                     /translation="MIKGIMFAFLAAFSWGAAIVMSKKGLENMDAGELFLWQVGSAAM
                     LSWFVLAISRKKLPVTKKSILAYSTGIFEPFLAYTFTLYGLKFISAGITAVI"
     gene            complement(281..463)
                     /locus_tag="ACHKIR_004071"
     CDS             complement(281..463)
                     /locus_tag="ACHKIR_004071"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9991747.1"
                     /translation="MIFNWNVLFYVSLEKFPVGNPYYGEIPGIPVDEEAKFFNPFPPR
                     AFENKPLYTEEITKYD"
     gene            complement(503..703)
                     /locus_tag="ACHKIR_004072"
     CDS             complement(503..703)
                     /locus_tag="ACHKIR_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9991748.1"
                     /translation="MKLDISVKYFLNIMIADFIIPAPEKISTTWHSDTKMNRFACPIH
                     STNSDYYSGFYHTDVLIPGELA"
     gene            822..1169
                     /locus_tag="ACHKIR_004073"
     CDS             822..1169
                     /locus_tag="ACHKIR_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6404 family protein"
                     /protein_id="HFO9991749.1"
                     /translation="MTFEQKKARAIALMDSKKMWRSNYAPPLLRILWRLGIRLPPLPF
                     MPFWQVTLLMGSLWGISWGCAMWFIYWGPSGMVAGEAIIISITGGFLFGLLTASFHWW
                     RRKVNRLPPWDDV"
     gene            complement(1187..1777)
                     /locus_tag="ACHKIR_004074"
     CDS             complement(1187..1777)
                     /locus_tag="ACHKIR_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9991750.1"
                     /translation="MTQSRQPSPLQRRVLIVLAALDEKRPGPVLTRDIERVLERSGEA
                     PVYGPNLRASCRRLEDVGWLRTLRAPNLQLAVELTDAGRAVAQPLLLAEQDRLRAEQR
                     AAEVVVLPLIPATGLPADGTSATDLAVELNGITYQACRGDFVVRLDGSTCLQLWNKEG
                     RVVRREGDPLEVAQWLQACHDAGMEVRVQVNESITP"
     gene            complement(1777..2034)
                     /locus_tag="ACHKIR_004075"
     CDS             complement(1777..2034)
                     /locus_tag="ACHKIR_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9991751.1"
                     /translation="MDEERVFSLSYEQLTRLAEKRIRECNLDSQGAIYLCESAKAGAV
                     LIFWHELAINGYASMNAIKRQELIDADFQRLRNLIWPEDDR"
     gene            2631..3044
                     /locus_tag="ACHKIR_004076"
     CDS             2631..3044
                     /locus_tag="ACHKIR_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9991752.1"
                     /translation="MFFIENEGQAVARTDYWQSVQAQAGYVYLSWNAGAARLLVPDAA
                     KHLLREMRGAEYVIISKGTLDGRDALELIFEDGSDAPFVIHMLSEQCDRLLPENNQGG
                     GFVVTVWTRGGNQLRYPGKYRVVENLPDVSPWSEH"
     gene            3046..3825
                     /locus_tag="ACHKIR_004077"
     CDS             3046..3825
                     /locus_tag="ACHKIR_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFO9991753.1"
                     /translation="MQHLPAPIHHARDAAQLPVAIDYPAALALRQMSMVHDELPKYLL
                     APEVSALLHYVPDLRRKMLLATLWNTGARINEALALTRGDFSLAPPYPFVQLATLKQR
                     TEKAARTAGRMPAGQQTHRLVPLSDAWYVSQLQTMVATLKIPMERRNKRTGRTEKARI
                     WEVTDRTVRTWIGEAVAAAAADGVTFSVPVTPHTFRHSYAMHMLYAGIPLKVLQSLMG
                     HKSISSTEVYTKVFALDVAARHRVQFSMPESDAVSMLKRIP"
     gene            4005..4649
                     /locus_tag="ACHKIR_004078"
     CDS             4005..4649
                     /locus_tag="ACHKIR_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFO9991754.1"
                     /translation="MPVIANTHPKGGVGKTTSSVNIVGEMKSDTVDLDTHTGLSIILG
                     LRPEGKEISVKVPKTVDELIEIMTPYKNSDKTLLIDCGGFDSDLTRTAIAFADCVIVP
                     SKDSLTERIGLMHFDGVLDEISSIMGTDITAHLYLCKVNPNKKKFPKLDAILPSFKHL
                     KLMKSRISARAEFEDVIETGMGITESVHGRYSAGGKEVIALIEEINHLIENNKQ"
     gene            4736..5044
                     /locus_tag="ACHKIR_004079"
     CDS             4736..5044
                     /locus_tag="ACHKIR_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone GroEL"
                     /protein_id="HFO9991755.1"
                     /translation="MSKAKFSAQAEKLKNLVDSARLQTSSSAVTATAPSQPNEGAEEV
                     SGRPVAARTKAKNLKAIPLSYFEAHAELKATSKTSLDFSSYIIEAIREKLERDGAIGQ
                     "
     gene            5457..6437
                     /locus_tag="ACHKIR_004080"
     CDS             5457..6437
                     /locus_tag="ACHKIR_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HFO9991756.1"
                     /translation="MKIFIDDGSTNIKLAWLEDGDVKTLISPNSFKPEWSFSLLDDAA
                     PANYEIDGEKFSFDPLSADAVVTTETRYQYSDVNVVAIQHALQQTGLKAQPVDVIVTL
                     PISEYLDANNQKNKQNIERKKKNVMREVRVQGSDAFVIRSVSVLPESIPAGFSVLAGL
                     EDDESLLIVDLGGTTLDVSHVRSKMTGITKTWCDPNIGVSLITSGVKEQMAVHANTRV
                     SSFQADNIIVHRNEPDYLSRRIYNAEQRESIINVINERQKLLIKRVNDVISRFTDYTH
                     VMCVGGGADIVAEAVKNLTKVPDERFYLSSSPQFDLVMGMIKMKGGVTNE"
     gene            6430..6846
                     /locus_tag="ACHKIR_004081"
     CDS             6430..6846
                     /locus_tag="ACHKIR_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFO9991757.1"
                     /translation="MSDENKSRRCSFELFPDERTGDKIADELIANEKLKERGRFMRAM
                     LVTGAAFAAIDKRLPLLISELLTENTTLDDINKVISSVIPGAFSVEKKLLELLEKQSG
                     LHTSVDCSTPLTEQSLSRNDGEDQTRRNAENMFGDD"
     gene            complement(6848..8122)
                     /locus_tag="ACHKIR_004082"
     CDS             complement(6848..8122)
                     /locus_tag="ACHKIR_004082"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0019985 - translesion synthesis [Evidence
                     IEA];
                     GO_process: GO:0042276 - error-prone translesion synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFO9991758.1"
                     /translation="MFALADINSFYASCEKVFRPDLRNEPVIVLSNNDGCVIARSPEA
                     KALGIRMGQPWFQVRQMRLEKKIHVFSSNYALYHSMSQRVMAVLESLSPAIEPYSIDE
                     MFIDLRGINHCISPEFFGHQLREQVKSWTGLTMGVGIAPTKTLAKSAQWATKQWPQFS
                     GVVALTAENRNRTLKLLGLQPVGEVWGVGRRLTEKLNALGINTALQLAQANTAFIRKN
                     FSVILERTVRELNGESCISLEEAPPAKQQIVCSRSFGERITDKDAMHQAVVQYAERAA
                     EKLRGERQYCRQVTTFVRTSPFAVKEPCYSNAAVEKLPLPTQDSRDIIAAACRALNHV
                     WREGYRYMKAGVMLADFTPSGIAQPGLFDEIQPRKNSEKLMKTLDELNQSGKGKVWFA
                     GRGTAPEWQMKREMLSQCYTTKWRDIPLARLG"
     gene            complement(8122..8559)
                     /gene="umuD"
                     /locus_tag="ACHKIR_004083"
     CDS             complement(8122..8559)
                     /gene="umuD"
                     /locus_tag="ACHKIR_004083"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFO9991759.1"
                     /translation="MSTVYHRPAAPSGDDSYVRPLFADRCQAGFPSPATDYAEQELDL
                     NSYCISRPAATFFLRASGESMNQAGVQNGDLLVVDRAEKPQHGDIVIAEIDGEFTVKR
                     LLLHPRPALEPVSDSPEFRTLYPENICIFGVVTHVIHRTRELR"
     gene            complement(8556..8804)
                     /locus_tag="ACHKIR_004084"
     CDS             complement(8556..8804)
                     /locus_tag="ACHKIR_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFO9991760.1"
                     /translation="MIRIEILFDRQSTKNLKSGTLQALQNEIEQRLKPHYPEIWLRID
                     QGSAPSLSVTGARNDKDKERILSLLEEIWQDDSWLPAA"
     gene            9222..10124
                     /locus_tag="ACHKIR_004085"
     CDS             9222..10124
                     /locus_tag="ACHKIR_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HFO9991761.1"
                     /translation="MYFSAEPAQITEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QTTEDVQFEPCPGLTAAGRGVVSPENIAFTRWLTHLQDGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDDVRDTITALFTAKRGDWCGFWSNEDVSVWWNRLCDNVLPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTEQYGVKWPCGYEVNISSQGDNFIKVDF
                     DTPWCQPESDVIAELSRRFSCTLEHWYAEQGCDFCGWQLYERGELVDVLWGELEWSSP
                     TDDDELPEVTGPAWIVDKVAHYGG"
     gene            10121..10432
                     /locus_tag="ACHKIR_004086"
     CDS             10121..10432
                     /locus_tag="ACHKIR_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9991762.1"
                     /translation="MKYDGDGRATARFFSGKGCRRAPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRLASVTVYASPSPFSDERPSSRFRGITLPSKRRRLRYSTVGLTRYRT
                     R"
     gene            10508..10579
                     /locus_tag="ACHKIR_004087"
                     /pseudo
     CDS             10508..10579
                     /locus_tag="ACHKIR_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(10661..12361)
                     /gene="ltrA"
                     /locus_tag="ACHKIR_004088"
     CDS             complement(10661..12361)
                     /gene="ltrA"
                     /locus_tag="ACHKIR_004088"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFO9991763.1"
                     /translation="MTAKNINAGAPTDIVTHWHNINWAKCRREVRRLQARIVKAIQEE
                     KAGKVKALQWLLTHSFSARAMAVKRVTDNRGKRTPGVDGKTWSKPGSKMKAIYTLKRR
                     GYKPLPLRRIYIPKSNGKKRPLGIPTMKDRAMQALYLMALEPVAETTADPNSFGFRPC
                     RSTADAIEQCFTTLHRADRAQWILEADIRSCFDEISHEWLIANIPTDTAILKRWLKAG
                     YIDLGKLYPTSAGTPQGGIISPTLANMVLDGLQPLLKKTFYRGGLNPEKINIIRYADD
                     FVITGISHDTLSEKVLPLLENFLAERGLTLSPEKTRITHISDGFDFLGMNIRKYNGKL
                     LIKPSPQKVKEFLKRVRQTIKDNPTVSQSTLIWHLNPVLKGWVRYYRHVVSKKIFQKV
                     SHEIWKALWRWACRRHPKKGTGWIRKKYFRSVNFRHWVFGTETGKLLPSGKRELLALY
                     EISDMPVKRHRQIKSTANPFDPSYESYFEARLKATMLESIQGYSRLRNLWLRQGGQCP
                     VCHLPVNATTGWQLRHIVPKSEGGLDNIGNLAMLHPACHHTAKHQGISVMKPALMWSY
                     "
     gene            12983..13594
                     /locus_tag="ACHKIR_004089"
                     /pseudo
     CDS             12983..13594
                     /locus_tag="ACHKIR_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052645.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
     gene            13595..13816
                     /locus_tag="ACHKIR_004090"
     CDS             13595..13816
                     /locus_tag="ACHKIR_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9991764.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMERRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELDSCFKD"
     gene            13776..14262
                     /locus_tag="ACHKIR_004091"
                     /pseudo
     CDS             13776..14262
                     /locus_tag="ACHKIR_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052646.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
     gene            14307..15077
                     /locus_tag="ACHKIR_004092"
     CDS             14307..15077
                     /locus_tag="ACHKIR_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9991765.1"
                     /translation="MNKTLNALVCRHARNLLLAQGWPEETDIEQLNSHYPGWISIYVR
                     LDVSRLATLLISRHDGVLPPVLASAMQKLTGTVAELVLSGSQWQALPVLPADGTQVSF
                     PYAGEWLTEEEIRAVLDAVRNAVRSICYQVADDARRIRAALTTTGQTLLTRQTRRFRL
                     VVKESDYPCWLDEDDENLPVVLDAILNRGARFSSVEMYLVCECVEHILASGLVCDVLR
                     IPDEPSRRWFDRDILREVVLEARDEIRSMADALAKIRK"
     gene            15491..15916
                     /locus_tag="ACHKIR_004093"
     CDS             15491..15916
                     /locus_tag="ACHKIR_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO9991766.1"
                     /translation="MQYAKPVTLNVEECDRLSFLPYLFGNDFLYAEAYVYALAQKMMP
                     EYQGGFWHFIRLPDGGGYMMPDGDRFHLVNGENWFDRTVSADAAGIILTSLVINRQLW
                     LYHDSGDAGLTHLYRMRDAQLWSHIEFHPECNAIYAALD"
     gene            15963..16382
                     /locus_tag="ACHKIR_004094"
     CDS             15963..16382
                     /locus_tag="ACHKIR_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFO9991767.1"
                     /translation="MYCTVKEIIREVLNTDVPDSECVFAVVLTRGDVRHIARDWNLSD
                     DELETVMQRLDDAFVYGACDRVVSDIVNELMEEKRVNRLVTVPAVLLEKVMVMAGSEI
                     YRLHAVGSENGGDGDAFVREEREIMRVMRQALDGENG"
     gene            16382..16573
                     /locus_tag="ACHKIR_004095"
     CDS             16382..16573
                     /locus_tag="ACHKIR_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9991768.1"
                     /translation="MNISTETREILRNYKAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQGSRNR"
     gene            16587..16888
                     /locus_tag="ACHKIR_004096"
                     /pseudo
     CDS             16587..16888
                     /locus_tag="ACHKIR_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386902.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(16888..16983)
                     /locus_tag="ACHKIR_004097"
                     /pseudo
     CDS             complement(16888..16983)
                     /locus_tag="ACHKIR_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         3863 a         4334 c         4569 g         4428 t
ORIGIN      
        1 aataacagct gttataccag cagaaataaa ttttaggcca tagagagtga atgtatacgc
       61 tagaaatggc tcaaaaattc ctgttgaata ggcaaggatt gatttttttg ttaccgggag
      121 tttttttctg ctaattgcga gaacaaacca tgataacatt gcagcactac caacttgcca
      181 taaaaacaac tctccggcat ccatgttttc gagacctttt ttgctcatca ctattgctgc
      241 tccccatgaa aaagcagcaa gaaatgcaaa cataattccc ttaatcatat ttagttatct
      301 cctcagtata aagaggttta ttttcaaacg ctcttggtgg aaatggatta aaaaactttg
      361 cttcttcatc cacaggaata cctggaattt ctccataata aggattgcca acaggaaact
      421 tttccagtga gacataaaac aatacattcc agttaaaaat cataggatca ttactatcaa
      481 catcatggat tatgatggcg tcctaagcca gttctccagg aattaataca tctgtatgat
      541 aaaaacctga atagtaatca ctattagtag aatgtatagg gcaagcaaag cgattcattt
      601 tagtatctga gtgccatgtg gttgatattt tttctggtgc gggaatgatg aaatctgcta
      661 tcataatatt aagaaaatac tttacagata tatccagttt catctggttt ctcctttatt
      721 gttatcgtgt accggtgcat gacaacataa tcacagcatg ataataataa tcaataacaa
      781 taagctgtgt cacgtttaca tcatggaacg gaggtgctcc tgtgaccttt gagcagaaaa
      841 aagcccgggc cattgccctg atggatagca aaaagatgtg gcggagcaat tatgcccctc
      901 ctcttctgcg tattctctgg cggctgggta tccggcttcc gccattaccc tttatgccgt
      961 tctggcaggt cacgctgttg atggggagct tatggggcat ttcctgggga tgtgcaatgt
     1021 ggtttatcta ctgggggccg tcaggaatgg tggcaggtga ggccattatt ataagtatca
     1081 cgggtggttt tttgttcgga ctgctcacgg cctctttcca ctggtggcgc aggaaagtta
     1141 accggctgcc accatgggat gatgtgtaaa ccgtgcaggc atgtttttac ggggtgatgc
     1201 tttcattaac ctgcacacgg acctccattc ctgcatcgtg acaggcctgc agccactgcg
     1261 ccacttccag cggatcgccc tcccggcgta ccactctgcc ttctttattc cataactgca
     1321 gacaggtgct gccgtcgaga cgcaccacaa aatccccacg gcaggcctga tacgttatgc
     1381 cgttcagctc caccgccaga tcagtggcag aagtgccgtc tgcgggcaga cctgttgccg
     1441 ggatcagcgg cagtacgacg acttctgccg cacgctgttc tgcccggagg cggtcctgtt
     1501 ctgccagcag cagaggctgt gccacggccc ggccggcgtc cgtcagctcc actgccagct
     1561 gcaggttcgg agcgcgcagg gtacgcagcc agccgacatc ctccagacga cggcaggagg
     1621 cgcgcaggtt tggcccgtag accggggctt ccccgcttcg ctccagcacc cgttctatgt
     1681 cccgcgtcag cacggggcct ggccgttttt catcaagggc agccagcaca atcagcactc
     1741 tccgctgcag tggcgacggt tggcggctct gggtcattac ctgtcatcct ccggccagat
     1801 gaggtttctg aggcgctgaa agtcggcgtc aatgagctcc tgtctcttta tggcattcat
     1861 tgaggcatag ccatttatgg ccagttcatg ccagaagatc agcacggctc cggctttggc
     1921 tgactcgcag agataaatcg cgccctggct gtccagattg cattcccgga tgcgtttttc
     1981 cgcaagccgg gtcagttgtt catagctgag tgaaaacacc ctttcttcat ccatcctgcg
     2041 cgctccatct gataatgttc tgacaggtaa aaatcaaatt ctatatgatt tttgtgttat
     2101 tgatttctga taatatcaga tattgtttca ttacggatga ttaaaatcat gtctgatatg
     2161 aatttatgtg tgttgcttaa ttatcatatc gcctgcccgt ttcccgccgc gcagatccgg
     2221 tttccggctt ttctgccgcg cagatccgaa aaccctgagt cacggatctt cctctccctc
     2281 acacaacgcc atctcctgtc agtacaacat gtcgtgctgc attcagctgc tcatgacact
     2341 atatattgtg ttatttccct gacctgtgat gcgtcgcgca ggggcggaaa acagcgatat
     2401 gattatttcc ccagcgtggt acacttccgg aaagttgtga tattcaggaa agtcggatct
     2461 gacggaaacg gctctccggt aatttaacgg cgtggttata tggatgcttg ttatcatggt
     2521 gatgatgata acggcatgat gttatcagac ggcgtgacgg taagggcagt gatgatggat
     2581 gacgttatcg catgaccgtc cctgcccgga aaagaaaaaa ggagtcaccc atgtttttta
     2641 ttgagaatga aggtcaggct gtcgccagaa cggattactg gcagtctgta caggcgcagg
     2701 ccggatatgt ctacctcagc tggaatgccg gcgcagccag gctgcttgtc ccggatgcgg
     2761 caaaacattt actcagggag atgcgggggg ctgagtacgt catcatcagt aagggaacgc
     2821 tggatggccg cgatgcgctg gaactgatat ttgaagacgg cagcgatgcg ccgtttgtga
     2881 tccacatgct gagtgagcag tgcgatcgcc tgctccccga aaacaaccag ggagggggtt
     2941 ttgttgtcac cgtctggacg cgtggcggta accagctccg ttatccggga aaataccggg
     3001 ttgtggaaaa cctgcccgac gtttccccgt ggagtgagca ctgatatgca gcacctgccg
     3061 gcaccgatcc accatgcccg ggatgccgct cagcttcctg ttgccatcga ttatccggca
     3121 gcgctggcac tccgccagat gtcgatggtt catgatgaac tgccaaaata cctgctggcc
     3181 cctgaagtga gcgccctgct ccattacgtg ccggatctgc gccgcaagat gctgctggcc
     3241 acactgtgga acaccggtgc gcgcattaat gaagcactgg cgctgacgcg gggggatttt
     3301 tcgcttgcgc ctccgtatcc gtttgtgcag cttgcgaccc tgaaacaacg gacggaaaaa
     3361 gccgccagga cggcaggaag aatgcccgcc ggtcagcaga ctcaccggct ggttccgctc
     3421 tccgacgcct ggtacgtcag ccagctgcag acgatggtgg ccacactgaa aatccccatg
     3481 gaacggcgta ataaacgaac aggcaggaca gagaaagcgc ggatctggga agtgacggac
     3541 agaacggtca ggacctggat tggggaggcg gttgccgccg ctgctgctga cggtgtgacg
     3601 ttctctgtcc cggtcacgcc acatacgttc cgccattcct atgcgatgca catgctgtat
     3661 gccggtatac cgctgaaggt cctgcagagc ctgatggggc ataagtccat cagctcaaca
     3721 gaggtctaca cgaaggtgtt tgcactggat gtggctgcac ggcaccgggt gcagttttcg
     3781 atgcctgagt ccgatgcggt gtctatgctc aaaagaattc cttaagctct gactttttta
     3841 aatccctttc ggatatctca tggatatatt atcggtattt catattctgt tgacatgcag
     3901 gaccggtata gagtaaaaaa acatctattg agtacctctt ggatatccga aaggtgtctt
     3961 agggggtctt tatgggtatt acatgtggat tctggagata cactatgcct gttattgcta
     4021 atacgcatcc caaaggtggt gtgggtaaga caacttcatc cgtaaacatt gttggtgaaa
     4081 tgaagtctga tacagttgat ctggatactc ataccgggct ttctatcatt ctggggctga
     4141 gacctgaagg aaaagaaatt tccgtgaaag tacccaaaac agtggatgag ttaatcgaaa
     4201 ttatgactcc ctacaagaac agcgataaga cgttacttat tgactgtggg ggatttgact
     4261 cagatcttac ccgtactgcg attgcctttg cagactgcgt tattgtccct tcaaaagact
     4321 ccctgactga acgtattggt ctgatgcatt ttgatggagt actggacgaa atcagctcta
     4381 tcatggggac cgatataact gctcacctgt atctctgtaa agttaatcct aacaagaaga
     4441 aattccctaa gcttgatgca atcctgccat cgttcaaaca tctgaagctg atgaagagcc
     4501 gcatttctgc tcgtgcagaa tttgaggatg tcattgagac tgggatggga atcactgagt
     4561 ctgttcatgg gcgctattcg gcaggaggta aggaagttat tgccctgatc gaagaaatca
     4621 atcatctgat tgaaaataac aaacagtaag gtgtatctat tgggtattca tatagtatcc
     4681 tttgggcttt taatggatat cttttaagta tctaacaaac atcctgggtt atctcatgag
     4741 caaagcgaaa tttagtgcac aggctgaaaa actgaaaaac ctggtggatt ctgccagatt
     4801 acaaacttct tcttctgccg taacagcaac agccccgtct cagccaaatg aaggtgcaga
     4861 ggaagtttca ggtcgtcccg tggctgccag gacgaaggct aagaatctta aggccatacc
     4921 gctgtcgtat tttgaagctc atgcggaact taaagcgacc agtaaaacat ctctggattt
     4981 tagctcatat attattgagg ctataagaga gaagcttgaa cgtgatggtg caataggtca
     5041 ataaactatc ggatacctta agggtatctg atgtgtgtct tttaaatgcg ttatggggat
     5101 tcttcacgta cctaaagagt ccccattaga catctttctg tttatggtga catgattcgt
     5161 caccgctttc ctttcctctc agtttcacca tttcattcct ttcccttaac ctgaaccatg
     5221 atgtccacga tgtttttata ttgcatttat gtatcacata taaccttcat aatgtttttg
     5281 tatctatgat ttcctctggt cttaaagttg tgcgtacaat gtacatgcaa tttatcgtgt
     5341 ttttatgttg catttgtcct ttcagaaaga ccgttttttc attataattg aatatacatt
     5401 gcatgtatga agcatgtttg ttttttctat ttgtttatga gcaacggaga ctggttatga
     5461 aaatctttat tgatgatggt tctaccaaca ttaaacttgc ctggttggag gatggtgacg
     5521 tgaaaacgtt aatcagccct aacagcttta aaccggaatg gtcttttagt ttactggatg
     5581 atgctgctcc ggctaattac gagatcgacg gcgagaagtt ttcatttgat ccattaagtg
     5641 ctgatgctgt cgtaacgact gaaacacgct atcagtacag tgatgtgaat gttgtggcca
     5701 ttcagcatgc gctacagcag accggcctga aagcgcagcc tgttgatgtc atcgtcacat
     5761 tgccgatctc tgaatacctt gacgctaaca atcagaaaaa taagcaaaac attgaaagaa
     5821 agaaaaaaaa tgtgatgcgt gaagtacggg tgcaggggag tgatgcattt gttattcgca
     5881 gtgtttccgt tcttcctgaa agtattcccg ctggtttcag tgttcttgcg gggcttgagg
     5941 atgatgaatc ccttttgatc gtagatctcg gtggtacaac tcttgatgtg tcgcatgttc
     6001 gtagcaaaat gacagggatt acaaaaacct ggtgtgatcc gaatatcggc gtgtccctga
     6061 ttacatccgg ggttaaggaa cagatggctg tacatgctaa tacaagagtg agctcattcc
     6121 aggctgacaa tatcattgtg caccgtaatg aacctgacta cctttcccgt cgcatttata
     6181 atgctgaaca gcgtgagtct atcattaatg ttatcaatga acggcagaag ttgcttatta
     6241 aacgagttaa tgatgtcatc agccgtttca ccgactatac acatgtaatg tgtgttggtg
     6301 gtggggctga cattgtggcc gaggctgtga agaacctgac taaggttcct gatgagcgct
     6361 tttatctgag ctcatccccg cagtttgatt tagtcatggg aatgataaaa atgaaaggtg
     6421 gtgtgactaa tgagtgatga aaataaatcc agacgctgct catttgaact tttcccggat
     6481 gaacggacag gagataagat agcagatgag ctcattgcaa atgaaaaact gaaggagcgg
     6541 ggacgtttca tgcgcgcgat gttagttact ggtgcggcgt ttgcagctat agataaacgt
     6601 cttcctttgc tgatatcgga gttactgaca gaaaatacga ctctggatga tattaataag
     6661 gtgatttcca gcgtaatacc aggtgctttt tcggttgaaa agaaattgct tgaattactg
     6721 gaaaaacaat ctggtctcca tacgtctgtg gattgcagta ctcctcttac agagcagagt
     6781 ctgtcgagaa atgacggtga agaccagacc agaagaaacg ctgaaaatat gttcggagat
     6841 gactgaacta acccagcctg gcaaggggaa tatctcgcca tttagttgta taacactgac
     6901 tcaacatttc ccgtttcatt tgccattcag gggcggttcc tcgccccgca aaccacactt
     6961 tcccttttcc cgactggttc agttcatcga gtgttttcat taacttttca ctgtttttac
     7021 ggggctggat ttcatcaaat aatcccggct gcgctatacc cgatggtgtg aaatcagcca
     7081 gcatgactcc tgccttcata tagcggtacc cttcacgcca gacatggttt aaggctctgc
     7141 atgcggcggc aataatgtcc cggctgtcct gtgtgggcaa tggaagcttt tccacagcgg
     7201 cattgctgta acagggttct tttactgcaa agggggatgt ccgtacaaat gtcgtcacct
     7261 gccggcaata ctgacgctcc ccacgtagtt tctctgcggc ccgctctgca tactgaacaa
     7321 cagcctggtg catggcatct ttgtctgtga ttcgttcacc aaaactgcga ctacagacaa
     7381 tctgctgttt tgccggtggt gcttcttcca gggatatgca ggactcgccg ttgagttcgc
     7441 gtaccgtacg ctcaagaatg acgctgaagt ttttccggat gaatgccgtg ttagcctgcg
     7501 ccagctgcag tgctgtgtta atacccagcg cattcagctt ttccgtcagt ctgcgtccta
     7561 ctccccagac ctcaccaact ggctgcagcc ccagtagctt caaggtccga ttacggtttt
     7621 ctgccgtcag cgcgaccact ccggaaaact gtggccattg ctttgttgcc cactgtgcac
     7681 ttttagccag cgtttttgta ggcgcaatgc ccacccccat ggtgagtcct gtccagctct
     7741 ttacctgttc cctgagctga tgaccaaaaa actccggaga gatgcaatga tttatccccc
     7801 gcaaatcaat aaacatttca tcaattgagt agggctcaat tgcgggagaa agcgactcca
     7861 gaacagccat gacccgttgg ctcatgctgt ggtacagcgc ataattgctg gaaaatacat
     7921 gtattttctt ctccaggcgc atctgtctca cctgaaacca gggctgcccc attctgatgc
     7981 caagggcttt tgcctccggg ctgcgcgcga tcacacagcc atcgttattg ctgagtacga
     8041 tgaccggttc gttgcgaagg tccgggcgga aaactttttc acatgaggcg tagaaactgt
     8101 tgatatcagc cagtgcaaac atcagcgtaa ctcccgcgtc ctgtgtatca cgtgagtgac
     8161 aacaccaaaa atacagatgt tttccggata cagtgtgcgg aactccgggc tgtctgaaac
     8221 cggctccagt gccgggcgtg ggtgcaacag cagtcgtttg acggtgaact ccccgtcgat
     8281 ctcagcgata acgatgtccc cgtgttgtgg tttttcggcc ctgtccacta ccagcagatc
     8341 accattctgc acgccagcct ggttcatcga ttcaccgctg gcgcgcagaa agaaggtggc
     8401 tgcaggtctg ctgatgcaat agctgttcag atccagttcc tgctcagcat aatcagtggc
     8461 aggagaagga aaaccggcct ggcaacgatc ggcaaacaac ggtcgcacgt aactgtcatc
     8521 gcctgaaggg gcagccggac ggtgataaac ggtactcatg ctgcaggcag ccagctgtcg
     8581 tcctgccaga tttcttccag cagagacagg atacgttctt tatccttgtc gttgcgggct
     8641 cctgtgacag atagagacgg ggcgctgccc tggtctatac gcagccagat ttccgggtag
     8701 tgtggtttca gacgttgttc gatttcattc tgtagcgcct gaagtgtgcc tgatttgagg
     8761 ttttttgtgc tctggcggtc gaaaagaatt tcaatgcgga tcatgttctc atctcccttt
     8821 tcttttgctg tatatgtata cagtattcta ataactgaat ccgcacacag tcaaatgtca
     8881 ttgttctgac taaaaagcga tcaattctct gagggggtgg ctgtatgcct gctgtggagt
     8941 gctgtatcca gcttatccac agcattttgt gcacggttct gtggacaaaa tacctggtta
     9001 cccaggccgt gccggcacgt taaccgggct gcatccgatg caagtgtgtc gctgtcgccg
     9061 gcctcctcac ccggtcacgt ttcgtcgttc ctcctccacg cgctgcggct tcggggccgc
     9121 acctgcattc gtatgcggtc gcccggttac aggtgcggca cggcctgatg gaggccgcat
     9181 gtgagaggag aattcctatg ccaaactggt gctcgaatcg tatgtatttt tctgccgaac
     9241 cggcacagat tactgagatt aaacgactgg ccagcggtgc agtcacaccg ctttatcgcc
     9301 gcgccacaaa tgaaggtatt cagctgtttc tggccggaag tgccggactt ctgcagacca
     9361 ctgaagatgt gcagtttgaa ccgtgccccg gactgacggc tgccggacgt ggtgttgtat
     9421 cgccggagaa tatcgcgttc acccgctggc tgacacacct gcaggacggt gtactgctgg
     9481 atgagcaaaa ctgcctgatg ctgcatgaac tctggctgca gagcggtact ggacagcgtc
     9541 gctgggaagg attaccggac gatgtcaggg ataccatcac cgcacttttc accgcaaaaa
     9601 gaggtgactg gtgtggcttc tggagtaacg aggatgtatc ggtgtggtgg aaccgtctgt
     9661 gtgacaacgt actgccggaa aaaaccatgc cgtttgacct gctgacggtt ctgccgaccc
     9721 gcctggatgt tgaagtgaat ggctttaacg gtggtgttct gaacggtgtt ccttctgcat
     9781 atcactggta tacggaacag tatggcgtga agtggccttg tgggtatgag gtgaatatca
     9841 gcagtcaggg agacaacttc attaaggtgg atttcgacac gccgtggtgt cagccggaaa
     9901 gcgacgttat tgcagaatta agccgccgtt tcagctgcac gctggagcac tggtatgccg
     9961 aacagggctg tgatttctgt ggctggcagt tgtatgagcg cggagagctc gttgatgtgc
    10021 tgtgggggga actggaatgg tcttccccga cagatgacga tgagctgccg gaagtcaccg
    10081 gacctgcgtg gatagtcgac aaggtggcgc attatggcgg atgaagtatg acggagacgg
    10141 gcgggcaacc gcccgtttct tttccggtaa aggatgtcgc cgtgctcctc tttttactgc
    10201 gccggctgac gcggcgcggc ataaacgctg cctgtggtca gtgtcccgcg tccggcgggc
    10261 acgggacggg cgcttttacc gctcccggct ggcgtcggtg acagtgtacg ccagcccgtc
    10321 gcccttttct gatgaacgcc cttcaagccg ctttcgcggc ataaccttgc cgtcaaaaag
    10381 acgacggctg cggtattcca cggtcggcct gacccgttac cggacgcggt gaacagccca
    10441 caggcagcgg ggaacgggca ccacaggggt gccctcccgg agccctctgt aagagaagga
    10501 gttttgtatg tcccgttttg tccttggtaa ctgcattgat gttatggccc gtattcctga
    10561 taacgccatt gatttcatgt cgggtagcag ctgcacatta ctgtgcagcc gccccctaag
    10621 aaccgtacgt gccagtttcc cggcatacgg ctcaagcctt tcaataactc cacattagag
    10681 ccggcttcat aacagatatt ccctgatgtt ttgcagtgtg atgacatgcc ggatgtaaca
    10741 tggccagatt acctatgtta tccagtcccc cttcggattt cggcacaatg tgtcgaagtt
    10801 gccagcctgt tgtggcgttc actggcaggt ggcagacggg gcactgacct ccctgtcgga
    10861 gccacagatt tcttaggcga gagtaaccct gtatggattc cagcatcgta gcttttagcc
    10921 gtgcttcaaa gtacgattcg tagctgggat caaaagggtt agcagtgctt tttatttgtc
    10981 tgtgtctttt gactggcata tccgagattt catatagcgc cagtaactca cgtttgcctg
    11041 atggcaggag cttccctgtt tctgtgccga atacccagtg tcggaagtta actgaacgaa
    11101 agtatttctt tcttatccag cctgtacctt tctttgggtg tcgtcgacat gcccagcgcc
    11161 atagtgcttt ccatatttca tgactgactt tctgaaagat cttttttgaa acaacatgac
    11221 gatagtatct gacccagcct ttgaggactg ggttgagatg ccatatcagt gtgctctggc
    11281 tgactgtcgg attgtctttt atcgtctggc ggaccctttt caggaattcc tttacctttt
    11341 ggggtgaagg tttaatcaac agtttcccgt tatatttgcg gatgttcata cccagaaagt
    11401 cgaatccatc actgatgtgt gtgattcgtg tcttttctgg tgagagggta agccctcttt
    11461 ctgccagaaa gttttctaac aagggaagaa ccttttctga cagcgtgtcg tggcttatcc
    11521 ccgtgatcac aaaatcgtcc gcataacgga ttatgttgat cttttctgga ttcagtccgc
    11581 ctcgatagaa ggttttcttc aacagaggct gcagaccatc cagaaccatg tttgccagcg
    11641 ttggagatat tattccgccc tgtggagttc ctgcactggt tgggtataat ttccccaaat
    11701 caatatatcc ggctttcagc catcgtttca ggatcgccgt atccgttggg atattggcga
    11761 ttagccattc atgtgaaatc tcatcaaaac aactgcgaat gtcggcttcc agaatccatt
    11821 gtgcccgatc agccctgtgc aacgttgtga aacattgctc aatggcatcg gctgttgagc
    11881 gacagggcct gaacccaaac gaatttgggt cagccgttgt ttctgcaaca ggttccagcg
    11941 ccatcagata gagtgcctgc atggctctgt ctttcattgt cgggatccct aatggtcgct
    12001 ttttgccgtt agattttggg atatatatac gccgtagcgg aagaggtttg taaccccttc
    12061 ttttcagcgt gtatatagcc ttcatcttgc tgccgggttt tgaccatgtt ttgccatcaa
    12121 cacccggcgt tctcttccct ctgttatctg tcactcgttt aactgccatt gctctggcgc
    12181 tgaacgagtg ggtgagtagc cattgcaagg cttttacctt gcccgctttc tcctcctgta
    12241 ttgccttcac gatacgcgcc tgcagccttc tgacctcgcg acggcacttg gcccagttga
    12301 tattgtgcca gtgagtcacg atgtcggtgg gtgcacccgc gtttatgttt ttcgcagtca
    12361 tctgcttacc ctccttcaaa ggtattcagg ttatctcgtg ttgaaagacc atgcggaagt
    12421 ctgcccgctt tcgcgtggaa tgatgttgct gaaacagctc aactcctgtc catcccatta
    12481 caggatggca ttcgctttct ccgcactcct gtacccgcat cactgacagc tttcctcgcg
    12541 gttagcctgc cctgagggca gtgatacggg cttaccgtgt tccgcacagt ttgcagggat
    12601 gggttaggtt ctgcctatcc accggtagct ttgatgtccg tgcctcccca gtcaaaagag
    12661 gaaggtccag ctacgttccc attttgggtc gggcctgtca gtgcctttgg cccgttcagg
    12721 gttgacgatg tttatcagca gttcacatgt gttaaccctg ccatccagcc ttgcgcccca
    12781 tccgccctga cactggcaga catgcttcct cctcgcggat tccacatggt gtgaaaaccg
    12841 ggctacgttg tcccgaaggc ttcataccga acagttaccc gctctgcatg cttcggtagg
    12901 ctacaggcga cggaacgcct ggttttacta cttcctttac atgaagttaa aacaacaaac
    12961 tgtgcgactt tcacgtcgca cccttaccga tcctccatat ctcgtcggtt tccgtgaccg
    13021 tcaggggcgc accattgccg gtgacgttaa tgatgactgg ctgcaacctg ccagcaatga
    13081 aatgtaccgc gtactcaaaa aagatgcgct gatggtgagt ttctatggct ggaaccgtat
    13141 tgaccgcttt atggcagcct ggaaacgcgc cggattcagc gtggcagggc accttgtgtt
    13201 caccaaaaac tacacatcca aatctgcgta tgtcgcgtac cgccacgaat gcgcttacat
    13261 cctggcaaag ggcagtccgg cccggccccg taacccgctg ccggatgtgc tgggctggaa
    13321 atattcgggc aaccgccatc acccgacgga aaagcccgtt accagcctgc aaccactgat
    13381 tgagagcttc acacatccga acgcgattgt gctggacccg tttgcaggca gcggctcaac
    13441 ctgcgtcgcc gccctccagt ccggacgcag gtatatcggt atcgagctgc ttgagcagta
    13501 tcaccgtgcc ggacagcaac gtctggctgc cgtacagcgg gccatgcagc agggggccgc
    13561 aaatgatgac tggtttatgc cggaggctgc gtaaatgaac tatgccggac atgaaaaact
    13621 tcgcgccgaa gtggcggagg tggccaatgc catgtgcgac ctgcgtacaa ccatgaatga
    13681 gatggagcgg cggtacagct ttaatgctga caccctgccg gaacgtctgg tgcgtcagac
    13741 gttgtttcgc gcaaaccgcc tcctgatgga ggcatatacc gaaattctgg aactggattc
    13801 ctgcttcaaa gattgagggg agagagtatg tacggaacat gcgaaatact ctgccgggag
    13861 ctggcagcaa agtatccggg agacacgccg ctgatgctgg ttatctggtc tccggaagag
    13921 attcaggccc ttgctgacgg aatggatatt tccctgtccg atcatgaaat caggaccgtc
    13981 ctggcgcgcc tggaggacat cccggaagac cagcggactg agtccggtat ttcttcgggt
    14041 gcggctatgg agattatcag caacgtgagt gaaaaccgcc aggtgaccgt ccctgctgaa
    14101 ctgctggcgt ccctgattca gaccgctgaa caggcattgt ggaagcgtga atgggccgcc
    14161 cgtgataacg gcctcgtcgt cccggaatgc gtcacccgcc gtcaggcggt ggttaatcag
    14221 gcccgcgccc tgctgaaaaa caacacacac gaaaacgact gatgttatcg ccgctttcgg
    14281 gcggcgattc agggagacca ttaatcatga acaaaacgct gaatgcactg gtttgtcgcc
    14341 acgcccgtaa cctgctgctg gcgcagggct ggccggaaga gacggatatt gaacaactga
    14401 attcgcacta tccgggctgg atcagcattt atgtgcgact ggatgtatcc cggctggcga
    14461 cgttacttat cagccgccat gatggcgtgc tgccgccggt cctggcgtct gccatgcaaa
    14521 aactgaccgg aaccgtggcg gaacttgtac tgtccggcag ccagtggcag gcgctgccgg
    14581 tacttccggc agacggaacg caggtgtctt tcccgtatgc cggtgaatgg ctgacggagg
    14641 aagaaatccg ggcagttctt gatgcggtgc gcaatgcagt acgaagcatc tgttaccagg
    14701 tggcagacga tgcgcggcgt atccgggcgg cgctgaccac caccggtcag acgttgctga
    14761 cccgccagac gcgccgcttt cgtctggttg tgaaggaaag cgattacccc tgctggcttg
    14821 atgaagatga cgaaaatctg cccgtggtgc tcgatgccat tctgaaccgg ggcgcccgtt
    14881 tttcctctgt ggaaatgtac ctggtctgtg agtgtgtgga gcatatcctg gccagtggtc
    14941 tggtctgtga tgtgctgcgt ataccggatg aaccgtcccg tcgctggttt gacagggata
    15001 ttctgcggga ggttgttctg gaggcacggg atgaaatccg cagcatggca gatgcactgg
    15061 cgaaaatccg gaaatgatga cgctggcggg gaaaaatctc cgccttttcc tgccggtccc
    15121 cggacagagc aaaaggatgt cgccgcaggc tccgctttac ccggccatgc ggggcatggc
    15181 cttgtgggtt ttcagttatg tggcctcagc gtcgtgtgcg ggctgtgccg tgcctccatc
    15241 ttagccgggc tggcggggat gcaagggtaa aatgcacgcg gcggtcaccc ggtccctcct
    15301 tcccgtctgc cgtgattttc cggtcgttca cccgctcaga tttcgcggtc tcaccctcca
    15361 actcccgtca gccgtgtgcg tcaggctgcg gcttcccttg cgcccttgca tccccgcctt
    15421 atcgcccggc ttttctggag gcacggcacc gcccggtgtc gcggaatatg tgaacaacgg
    15481 aggaaatccc atgcagtatg cgaaacctgt cactctgaac gttgaagagt gcgaccgcct
    15541 ttcctttctg ccttacctgt ttggcaacga ttttctgtat gccgaggcgt atgtgtacgc
    15601 gctggcgcaa aaaatgatgc cggaatatca gggaggcttc tggcatttca tccgcctgcc
    15661 ggatggtggc ggttacatga tgccggatgg cgaccgtttt catctggtca acggtgaaaa
    15721 ctggtttgac cgcactgtga gtgctgatgc cgcaggtatc atcctgactt cccttgtgat
    15781 taaccgccag ttgtggctgt accacgacag cggggatgcg ggactgacac acctttaccg
    15841 gatgcgtgat gcgcagttgt ggagccacat tgaatttcat cccgaatgta acgcgattta
    15901 cgcagcactg gactgattaa cggacggggc ggcgatgccg cccctgtaaa ggagacgaga
    15961 acatgtactg tactgttaaa gaaattatcc gcgaagtgct gaatacagac gtgccggaca
    16021 gtgaatgtgt ttttgccgtg gtgctgaccc gcggggatgt gcgccacata gcccgggact
    16081 ggaacctgtc agacgatgag ctggaaaccg tcatgcagcg gctggacgat gcttttgtat
    16141 atggcgcatg tgacagagtt gtttccgata ttgtcaatga actgatggag gaaaagcgcg
    16201 taaaccgtct tgtaacagtc cctgcggtgc tgctggaaaa agtgatggtg atggctggta
    16261 gtgaaattta tcgcctccat gctgtaggga gcgagaacgg gggcgacggt gacgcatttg
    16321 tcagggaaga acgtgagatt atgcgggtaa tgcgtcaggc gctggacggg gagaacgggt
    16381 aatgaatatc agcacggaaa cccgcgaaat tctgcgtaat tacaaagctg tgattaatgc
    16441 gcggcgtcgt gaaatggggc agaaaccgct caccaccgcg cagattgttg atgaaatctg
    16501 tgattttgtg gcgaatcagc aggcggtttt cctcggtggt cactatatcc ttcagggcag
    16561 cagaaacagg tgatacaggg caggcggtgg aggccgcctg tcagcccgcg cgaaaacccc
    16621 tggcaggctg gcgcctgccg gaagggaagc gggccacgct ggcgcgtcgc ccgttaccct
    16681 tcctgttggt gacaggcgtt gtttcaggaa gtacctgtta cagacctgtc gttaaagaac
    16741 cttcttacac atgcgcccct tcggctcccg gctgcctggc ggcatccggt cgtcggcccg
    16801 actacgacag gcagctgctg gcgcacccgc cagtctccgc cgaacccgct ggcgcggttt
    16861 cgggctgtcg ctacggtccg atgcgtgaaa acccgggctt gcagcgaccg gaacggtcgc
    16921 gccgcctttg tcgcgccgtg gcctgcgctc ctggcatccc ggcaaaaggc cgccgggatg
    16981 taaggaaacc gcgcctgatg gcgcggcagt ctcctcttct gccgccacct ctgcggcctg
    17041 tttcccgctg tggactttcc ggggggcaga cgtaaccacc tcccgttcca tcagtccagg
    17101 ggaagatata ccggccacaa aatttttagc catgaacccg gcaaaaaatt ttgcgtccgc
    17161 ccccggacag ctgtcccgtg aggcggtaaa cggg
//
LOCUS       DAZGQL010000105        17187 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.105, whole genome shotgun sequence.
ACCESSION   DAZGQL010000105 DAZGQL010000000
VERSION     DAZGQL010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17187
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.105"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            372..1202
                     /gene="efeU"
                     /locus_tag="ACHKIR_004098"
     CDS             372..1202
                     /gene="efeU"
                     /locus_tag="ACHKIR_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HFO9991769.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSVFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
     gene            1260..2387
                     /gene="efeO"
                     /locus_tag="ACHKIR_004099"
     CDS             1260..2387
                     /gene="efeO"
                     /locus_tag="ACHKIR_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HFO9991770.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDKYADQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKVNPELLAKVDANFIKVDTILAKY
                     RTKDGFENYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            2393..3664
                     /gene="efeB"
                     /locus_tag="ACHKIR_004100"
     CDS             2393..3664
                     /gene="efeB"
                     /locus_tag="ACHKIR_004100"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HFO9991771.1"
                     /translation="MQYEDENGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGVISDHA
                     ARSKGKETPINLLGFKDGTANPDSQNAKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTPESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFALPGVKDANDYLGSALLRV"
     gene            4290..5078
                     /gene="phoH"
                     /locus_tag="ACHKIR_004101"
     CDS             4290..5078
                     /gene="phoH"
                     /locus_tag="ACHKIR_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HFO9991772.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMSGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVRSGLSDALERFEEDEMVGIIRFGKEDCVRSALCQRTLHAYS"
     gene            complement(5128..5541)
                     /gene="pgaD"
                     /locus_tag="ACHKIR_004102"
     CDS             complement(5128..5541)
                     /gene="pgaD"
                     /locus_tag="ACHKIR_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     biosynthesis protein PgaD"
                     /protein_id="HFO9991773.1"
                     /translation="MNNLIITTRQSPVRLLVDYVATTILWTLFALFIFLFAIDLLTGY
                     YWQSEARSRLQFYFLLAVANAVVLIVWALYNKLRFKKQQHHAAYQYTPQEYAESLAIP
                     DELYQQLQKSHRMSVHFTSQGQIKMVVSEKTLVRV"
     gene            complement(5543..6868)
                     /gene="pgaC"
                     /locus_tag="ACHKIR_004103"
     CDS             complement(5543..6868)
                     /gene="pgaC"
                     /locus_tag="ACHKIR_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008375 - acetylglucosaminyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042710 - biofilm formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine synthase"
                     /protein_id="HFO9991774.1"
                     /translation="MINRIVSFFILCLVLCIPLCVAYFHSGELMMRFVFFWPFFMSIM
                     WIIGGVYFWVYRERHWPWGENAPAPQLKDNPSISIIIPCFNEEKNVEETIHAALAQRY
                     ENIEVIAVNDGSTDKTRAILDRMAAQIPRLRVIHLAQNQGKAIALKTGAAAAKSEYLV
                     CIDGDALLDRDAAAYIVEPMLYNPRVGAVTGNPRIRTRSTLVGKIQVGEYSSIIGLIK
                     RTQRIYGNVFTVSGVIAAFRRSALAEVGYWSDDMITEDIDISWKLQLNQWTIFYEPRA
                     LCWILMPETLKGLWKQRLRWAQGGAEVFLKNMTRLWRKENFRMWPLFFEYCLTTIWAF
                     TCLVGFIIYAVQLAGVPLNIELTHIAATHTAGILLCTLCLLQFIVSLVIENRYEHNLA
                     SSLFWIIWFPVIFWMLSLATTLVSFTRVMLMPKKQRARWVSPDRGILRG"
     gene            complement(6861..8879)
                     /gene="pgaB"
                     /locus_tag="ACHKIR_004104"
     CDS             complement(6861..8879)
                     /gene="pgaB"
                     /locus_tag="ACHKIR_004104"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase PgaB"
                     /protein_id="HFO9991775.1"
                     /translation="MLRNGNKYILMLVSIIMLTACISQSRTSFIPPQDRESLLAEQPW
                     PHNGFVAISWHNVEDEAADQRFMSVRTSALREQFAWLRENGYQPVSIAQIREAHRGGK
                     PLPEKAVVLTFDDGYQSFYTRVFPILQAFQWPAVWAPVGSWVDTPADEQVKFGDEMVS
                     REYFATWQQVREVARSRLVEVASHTWNSHYGIQANATGSLLPVYVNRAYFTDHARYET
                     AAEYRERIRLDAVKMTEYLRTKAEVNPHVFVWPYGEANGIAIAELKKLGYDMFFTLES
                     GLANASQLDSIPRVLIANNPSLKEFAQQIITVQEKPLQRVMHIDLDYIYDENRQQMDR
                     NIDVLIQRVKDMQISTVYLQAFADPDGDGLVKEVWFPNRLLPMKADIFSRVAWQLRTR
                     SGVNIYAWMPVLSWDLDPTLTRVKYLPTGEKKAQIHPEQYRRLSPFDDRVRAQVGMLY
                     DDLAGHAAFDGILFHDDALLSDYEDASAAAIAAYQQAGFSGSLSEIRQNPEQFKQWTR
                     FKSRALTDFTLELSARVKAIRGPHVKTARNIFALPVIQPESEAWFAQNYADFLKSYDW
                     TAIMAMPYMEGVTEKSADQWLTQLTNQMKSIPQAKDKSILELQAQNWQKNGQHQAISS
                     QQLAHWMSLLQLNGVKNYGYYPDNFLHNQPEIDVIRPEFSTAWYPKND"
     gene            complement(8888..11311)
                     /gene="pgaA"
                     /locus_tag="ACHKIR_004105"
     CDS             complement(8888..11311)
                     /gene="pgaA"
                     /locus_tag="ACHKIR_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1901515 -
                     poly-beta-1,6-N-acetyl-D-glucosamine transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine export
                     porin PgaA"
                     /protein_id="HFO9991776.1"
                     /translation="MYSSSRKRCPKTKWALKLLTAAFLAASPAAKSAVNNAYDALIIE
                     ARKGNTQPALSWFAQKSTLSNNQIADWLQIALWAGQDKQVITVYSRYRHQQLPARGYA
                     AVAVAYRNLQQWQNSLTLWQKALSLEPQNKDYQRGQILTLADAGHYDTALVKLKQLKS
                     GAPDKANLLAEAYIYKLTGRHQDELRAMTESLPENASKQQYPTEYVQALRNNQLAAAI
                     DDANLTPDIRADIHAELIRLSFMPTRSESERYAIADRALAQYAALEILWHDNSDRTAQ
                     YQRIQVDHLGALLTRDRYKDVISHYQRLKNTRQIIPPWAQYWVASAYLKEQQPKKAQS
                     IMTELFYHKETIAPDLSDEELADLFYSHLESENYPGALTVTQHTINTSPPFLRLMGTP
                     TSIPNDTWLQGHSFLSTVAKYSNDLPQAEMIARELAYNAPGNQGLRIDYASVLQARGW
                     PRAAENELKKAEVIEPRNINLEVEQAWTALTLQEWQQAAVLTHDVVEREPQDPGVVRL
                     KRAVDVHNLVELRIAGSTGIDAEGPDSGKHDVDLTTIVYSPPLKDNWRGFAGFGYADG
                     QFSEGKGIVRDWLAGVEWRSRNIWLEAEYAERVFNHEHKPGARLSGWYDFNDNWRIGS
                     QLERLSHRVPLRAMKNGVTGNSAQAYVRWYQNERRKYGVSWAFTDFSDSNQRQEVSLE
                     GQERIWSSPYLIVDFLPNLYYEQNTEHDTPYYNPIKTFDIVPAFEASHLLWRSYENSW
                     EQIFSAGVGASWQKHYGTDVVTQLGYGQRISWNDVIDAGATLRWEKRPYDGDREHNLY
                     VEFDMTFRF"
     gene            11899..13266
                     /locus_tag="ACHKIR_004106"
     CDS             11899..13266
                     /locus_tag="ACHKIR_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001526835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFO9991777.1"
                     /translation="MEKDYLGISSTVLVSLLFGLALVLVNSWFNQPTVEEVVPRSTYL
                     MVMIALFFIDTVAFIFMQLYFIYDRRQFSNCILSLAFLSCLIYFIKTVIIIQQIIEGR
                     LTSSVVQNDIAIYYLFRQMSLCVLIFLALVNKVSENTKQRNLFSKKVTLCITLFFVLG
                     GPIVAHIFSSHYESYDLHIAELTNKNGQVVWKASYVTIMIFMWLTLLSVNLYFNGLRC
                     DIWNGVSVIAFCAVLYNISLLFMSRYSVSIWYISRTIEVVSKLTVLVIFMCHIFSALR
                     VTKDIAHRDPLTNIFNRNYFFNELTVQSASAKKKPYCVMIMDIDHFKKVNDTWGHPVG
                     DQVIKTVVNIIGKSIRPDDLLARVGGEEFGVLLTEIDTECAKALAERIRENVERLTGD
                     NPEYAIPQKVTISIGAVVTQKNELNPKEIYQLADNALYEAKETGRNKVVVKEAEILIN
                     RKDDE"
     gene            13543..14751
                     /locus_tag="ACHKIR_004107"
     CDS             13543..14751
                     /locus_tag="ACHKIR_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000279862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO9991778.1"
                     /translation="MAVLTDTKARHIKPDDKPLPHGGITGLTLHPSSVKGRGKWVFRY
                     VSPVTQKRRNAGLGTYPEVSIAEAARTARIMREQLAAGDDPLEIKKAEAEKVVIPTFA
                     DAARRVHAELSPGWENPKHVRQWLSTLENYVFPQLGAKTLDTITAADVAETLRPVWLT
                     LSETASRVKQRIHVVMQWGWAHGFCVANPVDVVDHLLPQQSRGRDEHQPAMPWRQLPL
                     FVATSVYTDEPYNVTRALLLMVILTATRSGEARGMRWAEIDFHKRIWTIPAERMKARI
                     QHRVPLSRQAIHVLENIRGLHDELVFPSPRKQQILSDMVLTSFLRKKKAISDIPGRVA
                     TAHGFRSTFRDWCSEQGYSRDLAERALAHTLKNKVEAAYHRTDLLDQRIPMMQAWADY
                     VMSQIMENQR"
     gene            complement(14975..16540)
                     /locus_tag="ACHKIR_004108"
     CDS             complement(14975..16540)
                     /locus_tag="ACHKIR_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HFO9991779.1"
                     /translation="MTKLSPPELDITKLPDRCQALLDEMHEETGISREILLSIMLTVK
                     AASVQDTHEVELSGGQRTSLQIYMCLSSASGSGKTSACTKLIAPVHETEEELHQAYID
                     DKKNYDRMMEMWTTDKKILERRYKKEMERSPENAAAARAALEKCIANKPVPPVQQVLI
                     VNDATPEGIALKLSQSPSLLLLSDEGGTILDKRFERKSALYNTLWSGQPVTVERASRP
                     GFRVKDSRLTMLILTQPVIFDKFFTLTGDQIRGNGFLARVLFCEPGANKIMTTEPHTA
                     TPVVTQQCATCFHEKSFGSQIRDSLRASRERRAKGEQRICMTLSNAASHDLEIFHKEN
                     MSAVRQNPRMTTFEDIIVRKREQAVRIAALLELENNPHGTVITRESINSAIYLVDFYF
                     QHLISKLESLREISPAEKLDKWLRENIIRVKGYEYQKSHILQYGPYALRNKCVLDEAL
                     DILAEQKKIVIDYSIGQKIIYIGDAITPCELANEANIPIMERGMFMVCWDHKLNKYRN
                     EEQTKIWNITTKN"
     gene            complement(16556..16804)
                     /locus_tag="ACHKIR_004109"
     CDS             complement(16556..16804)
                     /locus_tag="ACHKIR_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001349431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO9991780.1"
                     /translation="MSTQTGHFYTPSRILRMAELTRLLGISRSTIYEKMNPASKYYDA
                     TFPRPVRLGSSAVGWRASAIDEWLTLHTTPTRSVTKDK"
     gene            16905..>17187
                     /locus_tag="ACHKIR_004110"
     CDS             16905..>17187
                     /locus_tag="ACHKIR_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001242275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9991781.1"
                     /translation="MRMFTNLLYDICTVFELFKEGESPRDKRKSTDFGAHQRFWDQRY
                     NELSHIIDAEGVYSLEQRRIIFSRYEYFYYMMNSYPVYSTLKSEYIRNYF"
BASE COUNT         4488 a         3763 c         4129 g         4807 t
ORIGIN      
        1 acacccgagc tgttattatg ttccgggcaa aaagttagat ttgataatcg cggatggacg
       61 aaattgcttg atacacccgc ttatcagttt tacatggaag ctctgatgca ttgagtctgg
      121 acagttttgt cggctggata aggcatttac gcggcatctg gcaagaacac atggctcgtt
      181 gtaaacaatc ccatctttgt acctggaata atcgtttata tcccctggca ttacctttct
      241 ttgtttacat tccaacatca ttttataaac attctgcttg tatttttctt taccgtaatg
      301 ataatcgcta tcactgcgat ttacttttct ttgcatagat tgactcagaa aaacgtttaa
      361 gggtgggtgg catgtttgtt ccgtttctca ttatgttgcg cgaaggactt gaagccgcgc
      421 tgattgtcag tttgattgcc agctatctta agcgtaccca gcgaggccga tggattggtg
      481 tgatgtggat tggcgtgttg cttgccgctg cgttgtgcct gggcttgggt atcttcatta
      541 acgaaaccac cggcgaattt ccgcaaaaag aacaggaact gtttgaaggt atcgtggcgg
      601 tgatcgccgt ggtgatcctt acctggatgg ttttctggat gcgcaaagtg tcgcgcaacg
      661 tcaaagtaca actggagcag gcagtcgata gcgcattgca gcgtggaaat catcatggct
      721 gggcgctggt gatgatggtc ttttttgccg ttgcaaggga agggctggag tcggtctttt
      781 tcctgctggc ggcgtttcaa caagatgtcg ggatctggcc gccgctgggt gcaatgctcg
      841 gtcttgctac agccgtggtg ctcggcttcc tgctctactg gggcggtatt cgcctcaatc
      901 ttggtgcatt ttttaaatgg accagcctgt ttattctctt cgtcgccgca gggctggcgg
      961 ctggtgccat tcgcgcattt catgaagccg gattgtggaa ccactttcag gaaatcgcct
     1021 tcgatatgag tgcggtgctc tcaactcact cggtgtttgg cacgctgatg gaagggattt
     1081 ttggctatca ggaagcgccg agcgtcagcg aagtcgccgt ctggtttatt tatctcatcc
     1141 cggcgctggt ggcatttgct ctgccaccac gcgcaggggc gacagcgtct cgctccgcgt
     1201 aacaaatacg acgcaaactc ttgcttagtt acaacatact ttaaagggat agtctcgtca
     1261 tgaccattaa cttccgccgt aacgcattgc agttgagcgt ggctgcgctg ttttcttctg
     1321 cttttatggc taacgccgct gatgtgccgc aggtcaaagt gaccgtgacg gataagcagt
     1381 gcgaaccgat gaccattacg gttaacgccg ggaaaacaca gttcattatt cagaaccaca
     1441 gccagaaagc actggagtgg gagatcctca aaggcgtgat ggtggtggaa gagcgggaaa
     1501 atatcgcccc tggttttagc cagaaaatga cggcaaattt acagcctggt gaatacgata
     1561 tgacctgcgg tctgctgaca aacccgaaag ggaagttgat cgtcaaaggt gaggcaacgg
     1621 cggatgcggc gcaaagtgat gcgctgttaa gtcttggtgg tgcaattact gcatataaag
     1681 cgtatgtcat ggcggaaacc acgcagctgg tgaccgacac caaagccttt accgatgcta
     1741 ttaaagcagg cgatatcgaa aaagcgaaag cactgtatgc gccgacgcgc cagcactatg
     1801 agcgcattga accgattgct gaactgttct ccgatctgga tggcagcatt gacgcccgtg
     1861 aagatgatta cgagcaaaaa gccgccgatc cgaaattcac cggtttccac cgtctggaaa
     1921 aagcattgtt tggcgataac accaccaaag ggatggataa gtatgccgac cagctttata
     1981 ccgatgtggt cgatttgcaa aaacgcatca gtgaactggc tttccctcct tcaaaagtgg
     2041 tcggcggtgc agccggactg attgaggaag tggcggccag caaaatcagc ggtgaagaag
     2101 atcgctacag ccacaccgat ctgtgggatt tccaggctaa cgttgaaggc tcgcagaaaa
     2161 ttgtcgatct gctgcgtcca caactacaaa aagtgaatcc ggaactgttg gcaaaagtcg
     2221 atgccaactt cataaaggtc gataccattc tggcgaaata ccgtactaaa gacggttttg
     2281 aaaattacga caaactgacc gatgctgacc ggaatgcact gaaaggaccg attactgcgc
     2341 tggcggaaga tctggcgcaa cttcgcggtg tgctggggct ggactaagcg ttatgcagta
     2401 tgaagatgaa aacggcgtga atgaaccgtc acgccgacgt ttactgaaag ggattggtgc
     2461 gctggcgctg gcgggaagtt gtccggtcgc tcatgcacaa aaaacgcaaa gtgcgccggg
     2521 tacgctttca ccggatgcac gcaatgagaa acagccgttt tatggtgagc atcaggcagg
     2581 gatcctgacg ccacaacagg ctgcaatgat gctggtggcg tttgatgtgc ttgccagcga
     2641 taaagccgat cttgaacggt tgtttcgctt gttgactcag cgttttgctt ttctgactca
     2701 gggcggagcg gcaccagaaa cgccaaatcc gcgcctgcca ccactcgatt ccggcattct
     2761 tggcggctac attgcgcccg ataatctcac cataacgtta tcggtgggcc actcattgtt
     2821 tgatgagcgc tttggccttg cgccacagat gccgaaaaag ttgcagaaga tgacgcgttt
     2881 ccccaacgac tcgctggatg cggcgttatg tcatggtgat gtgttgctac aaatttgcgc
     2941 caacacccag gacacggtga tccatgcgct gcgcgatatc atcaaacaca cgccggattt
     3001 gctcagcgtg cgctggaagc gggaaggggt tatttccgac cacgcggcgc gtagtaaagg
     3061 taaagagaca ccgattaatt tgctgggttt caaagacggc actgccaatc ccgatagcca
     3121 gaatgcgaag ttgatgcaaa aagtggtgtg ggtgacggca gatcagcagg agcctgcgtg
     3181 gacaatcggt ggtagctacc aggcggtgcg gttgattcag tttcgggtgg aattttggga
     3241 cagaacgccg ctgaaagaac agcagacgat atttgggcgt gataagcaaa ccggcgcgcc
     3301 gctgggaatg cagcatgagc atgatgtgcc tgattacgcc agcgatccgg aggggaaggt
     3361 gatcgcgctg gacagccata tccggctggc gaatccccgc acgccagaga gtgagtcaag
     3421 tctgatgctg cgtcgtggct acagttattc gttgggcgtc accaattccg ggcaactcga
     3481 tatggggcta ctgtttgtct gctaccaaca cgatctggaa aagggcttcc tgacagtaca
     3541 aaaaaggttg aatggagagg cgctggagga atatgtcaaa cctatcggtg gcggctattt
     3601 ctttgcgctg ccgggtgtga aggacgcgaa cgattatctc ggaagcgcat tattgcgggt
     3661 ttaatgtttt taggcggata aggcgtttac gccgcaccag gcgttcgtgc gctgatgcga
     3721 cgcttgcgcg tcttatcatg cctacaatcg gtgcgggttc ggtaggccgg ataaggcgtt
     3781 cacgccgcat ccggcaagct tttaccagta ctgcgaatcc tgctgaaagc acacagcttt
     3841 tttatcactg tcatcacttt gtcatctttc cagtagaaac taatgtcact gaaatggtgt
     3901 tttatggtta aatataagtg aatatattgt tgcaatgaat gcgagatctg ttgtacttat
     3961 taagtagcag cggaagttcc cggcagtgat agtcagtcac tatggagatc gcggatggta
     4021 acgtcctgtt ctggacaatg ttttgttaag caaggctcca ccactcgcgg agatttctcc
     4081 tccggtagca acttcgtcac tctgtatttt tctcacttcc tctctttact cgtgttaccg
     4141 acgccgcaaa cggtgtgcgt agccgttttt cggcatttta tccaaaagca aactatggct
     4201 tacaaggaag ccaaccctct gatgttcgtg cgcataatcg cgctgccaac ggcgcgtgtg
     4261 atgaatacaa acaactcaag gtgctctcca tgggaagaca aaaagcagtg atcaaagctc
     4321 gtcgcgaggc aaaacgtgtg ctgagacggg attcgcgcag ccataagcag cgtgaagaag
     4381 aatcggtcac ctcgcttgtg caaatgagcg gcgtagaagc cattggtatg gcccgcgaca
     4441 gtcgcgatac ctcgcccatc ctcgcgcgaa atgaagcgca attgcactat ctgaaggcta
     4501 ttgagagtaa gcagctgata ttcgccacgg gcgaagccgg gtgcggaaaa acctggatca
     4561 gcgcagcaaa agcggcagag gccctgatac ataaggatgt cgacaggatt atcgtcaccc
     4621 gtccagttct gcaagccgat gaagatcttg gctttttacc tggagatatc gcagaaaagt
     4681 ttgctcccta ttttcgcccg gtttacgacg tgctggtccg gcgcttaggg gcttccttta
     4741 tgcagtactg cctgcgacca gaaataggga aggtcgaaat cgcgccgttc gcctatatgc
     4801 gtggacgtac ctttgaaaat gcagtcgtca ttcttgacga ggcgcagaat gtgactgccg
     4861 cgcaaatgaa aatgttttta acccgcctcg gggagaacgt gacggttatc gtcaacggtg
     4921 atatcacgca atgcgattta cctcgcggtg tgcgctccgg attaagtgac gcgctggaac
     4981 gtttcgaaga agatgaaatg gtcgggatca tccgattcgg taaagaggat tgcgtacgtt
     5041 cggcactttg ccaacgtacg ctacatgcat acagttaagt gtattatcgg tgcagagccc
     5101 gggcgaaccg ggctttgttt tgggtgttta tacccggact agcgtttttt ctgaaaccac
     5161 catttttatt tgtccctgac tggtgaagtg tacgctcatc ctgtggcttt tttgtagttg
     5221 ctgataaagc tcatcaggta ttgctaagct ctctgcatat tcttgcggcg tatattggta
     5281 tgctgcatga tgctgctgtt ttttaaaacg cagcttattg tacaatgccc agacgattaa
     5341 cacgacggca ttcgccactg ccagcaaaaa atagaactga agtcggcttc tggcctcgct
     5401 ttgccagtaa taacccgtca gcagatcaat ggcgaataag aatatgaaca aagcaaataa
     5461 tgtccacaag atggttgtgg caacataatc aaccagtaaa cgtactggcg attgtcgggt
     5521 cgtaataatt agattgttca tattaacctc tcagaatccc acgatcggga cttacccaac
     5581 gagcgcgttg ttttttaggc atcaacatga ctcgtgtaaa tgataccaat gtcgttgcca
     5641 ggcttagcat ccagaaaata accggaaacc aaataatcca gaaaagcgat gaagccagat
     5701 tgtgctcata gcgattctca attaccagac tgacaataaa ttgcagtaaa cataacgtgc
     5761 acaataatat tccggcagta tgtgtcgctg cgatatgtgt caattcaata tttaacggta
     5821 cacctgcaag ttggactgcg taaataatga aaccgaccag gcaggtaaag gcccatattg
     5881 tcgtcaggca gtattcaaaa aacagtggcc acattcgaaa gttttctttt cgccacaacc
     5941 ttgtcatatt tttgaggaat acttctgcac cgccctgagc ccagcgcagg cgctgtttcc
     6001 acagcccttt taacgtttca ggcattaata tccagcacag cgcccgtggc tcgtaaaaaa
     6061 tcgtccactg attcaactgc agcttccagc taatatcaat atcttcggtg atcatatcgt
     6121 cactccagta acccacttct gccagggcgc tgcgacgaaa tgcggcaata acaccggaaa
     6181 cggtaaatac atttccatag atacgctggg tccgcttgat caaaccaata attgaggaat
     6241 actcgccaac ctgaatttta cccactaggg tagaacgtgt gcgaatacga ggattaccgg
     6301 ttacggcacc cacacgtggg ttgtacaaca tcggttccac aatatatgcc gctgcatcgc
     6361 ggtctaataa cgcgtcgcca tcaatgcaca ccagatattc acttttcgcc gcggcagctc
     6421 cggttttaag cgcaatggct ttcccctggt tttgcgccag atgaatgacc cgcagacggg
     6481 gaatttgtgc agccatgcga tccaggatgg cacgggtttt gtctgttgaa ccatcattta
     6541 cggcaataac ttcaatgttc tcataacgct gtgctaaagc ggcatgtatg gtttcctcaa
     6601 cgtttttctc ctcattaaaa cagggaatga taatggagat agatggatta tctttcaact
     6661 ggggggctgg tgcgttttct ccccacggcc agtggcgttc acgatagacc cagaaataga
     6721 cgccgccaat aatccacata atggacataa aaaacggcca gaagaaaacg aacctcatca
     6781 tcagttcacc agagtgaaag tacgctacgc atagaggtat gcataacacc agacataata
     6841 taaaaaacga tacgatgcga ttaatcattt ttcggatacc aggctgttga aaactcagga
     6901 cgaataacgt ctatttcagg ttggttatgc agaaaattgt cgggataata accataattt
     6961 ttcactccat tcagttgtaa taggctcatc cagtgagcga gttgttgtga agaaatagcc
     7021 tgatgctgac catttttctg ccagttttgt gcctgtaatt ctaaaattga tttgtcttta
     7081 gcctgcggga tgcttttcat ctgattggtc aattgtgtta accattgatc agccgatttt
     7141 tctgtgacgc cttccatata aggcatagcc ataatagcgg tccagtcata gctttttagg
     7201 aaatcagcat aattctgtgc aaaccaagct tcactttcag gttgtattac cggaagtgca
     7261 aaaatatttc gtgcagtttt aacatgtgga ccacgaatgg cttttacgcg cgcactaagt
     7321 tctaaagtga agtcagttaa cgcacgactt ttaaagcggg tccactgttt gaattgctcc
     7381 gggttttgtc gaatttcgct cagactcccg ctaaagccag cttgctgata agccgcgata
     7441 gccgctgcac tggcatcttc ataatctgaa agcaaagcat catcgtggaa caatatgcca
     7501 tcaaaagcag catgtccggc aagatcatca tataacatgc caacttgtgc tctgaccctg
     7561 tcatcgaaag gggagagacg gcggtattgt tcaggatgaa tttgtgcttt tttctcccca
     7621 gttggtaagt attttactcg cgttaatgtg ggatctaaat cccagcttaa taccggcatc
     7681 cacgcataga tgtttacacc tgagcgggta cgcaattgcc aggcaacccg actaaaaata
     7741 tctgctttca ttggtagcaa acgatttgga aaccagacct ctttaaccag cccatcacca
     7801 tcgggatcag caaatgcttg taaatacacg gtagatattt gcatatcttt taccctctga
     7861 attagcacat caatattgcg atccatttgc tggcggtttt cgtcataaat gtaatcaaga
     7921 tcgatatgca ttacccgttg tagtggtttt tcctgtacgg taataatttg ctgggcaaac
     7981 tcttttaatg agggattatt ggcgattaat actcgcggaa tggaatctaa ttgcgacgca
     8041 tttgccaaac ctgattcaag ggtgaagaac atgtcataac cgagtttttt taattccgcg
     8101 attgctatgc cattcgcttc gccataaggc caaacaaaaa cgtgtggatt tacctcagcc
     8161 tttgtacgca ggtattccgt catttttaca gcatccagac gaattctttc ccggtattct
     8221 gctgcggttt cataccgtgc gtggtcagta aaatatgcac gatttacata tacaggcaat
     8281 aagctgccgg tggcattagc ctgaataccg tagtgagaat tccatgtatg agaagcgacc
     8341 tcaacgagcc gggaacgcgc aacttctcgc acttgttgcc acgtggcaaa atattctcga
     8401 ctgaccatct catcgccaaa ttttacttgt tcatccgctg gcgtatcgac ccaactgccg
     8461 acgggggccc atacagcagg ccactggaag gcctgaagaa ttgggaagac gcgggtataa
     8521 aaactctggt agccgtcatc aaaagtcagc actactgctt tttccggtag cggttttcct
     8581 cctcgatgtg cttcacgaat ttgagcaata ctgaccggtt gataaccgtt ctcgcgcagc
     8641 caggcaaatt gttcacgcag tgctgatgtc cgcactgaca taaaacgctg gtcggcagct
     8701 tcgtcttcaa cgttatgcca tgaaatcgcc acaaaaccat tatgcggcca cggttgctcg
     8761 gcgagtaaag attcgcgatc ctgtggcggt ataaatgatg ttcttgactg gctaatgcac
     8821 gcagtgagca taattatact caccagcatc aggatatatt tatttccatt acgtaacata
     8881 tttatcctta aaatctgaat gtcatatcga attcaacgta taagttgtgt tctctgtcac
     8941 cgtcataagg tcgtttttcc cagcgtagcg ttgcgccagc atcaatcacg tcattccagc
     9001 taatacgttg cccgtagccg agttgggtga cgacatccgt gccataatgt ttttgccagg
     9061 aggcaccaac acctgcgctg aagatttgct cccagctatt ttcatagctt cgccataaca
     9121 aatgacttgc ctcaaatgcc ggaacaatat cgaacgtttt tatagggttg tagtatggag
     9181 tgtcgtgttc tgtattttgt tcgtaataca gattgggtag gaaatcgaca atcaaatatg
     9241 gtgaagacca gatgcgttcc tgaccctcaa gtgagacttc ctgacgctgg ttactgtcgg
     9301 aaaaatcagt gaaagcccag gagacaccgt acttacgccg ctcattttga taccagcgaa
     9361 cataagcctg agcactgttg cctgtaacac catttttcat tgcccgtaat ggaacgcggt
     9421 gagagaggcg ttccagttgc gaaccaatac gccagttatc attaaaatca taccagccag
     9481 acaggcgcgc gccgggttta tgctcatgat tgaaaacgcg ttcagcgtac tccgcctcga
     9541 gccagatatt acgtgaccgc cactcaacac ccgccagcca gtcgcgaaca atcccttttc
     9601 cttcgctaaa ttgtccatcg gcataaccga atccagcaaa accgcgccag ttatccttca
     9661 gcggtggtga ataaacgatg gtggttaagt cgacatcatg tttaccacta tccgggcctt
     9721 cggcatcaat tcctgttgaa ccagctatac gaagctctac aagattatgt acatcaaccg
     9781 cacgttttaa acgtacaacg ccgggatctt gcggttcacg ttcgacaaca tcgtgcgtta
     9841 agacagctgc ctgctgccat tcttgtaacg ttaatgctgt ccatgcttgt tcaacctcca
     9901 gattaatatt acgtggctcg atcacttctg ctttttttaa ttcattttct gctgcacgag
     9961 gccaaccgcg ggcttgtaac acactcgcgt aatcaatgcg cagtccctga tttcctggtg
    10021 cgttataagc aagctctctg gctatcattt cagcctgagg aagatcatta ctatattttg
    10081 ctacggttga gagaaacgaa tgcccctgta accaggtatc attcgggatg ctcgtaggcg
    10141 tgcccattaa ccgaaggaaa ggcggcgaag tattaatggt atgttgggtg acagttagcg
    10201 cgccgggata attttcactc tccaggtggc tgtaaaagag atccgcaagt tcttcatcgg
    10261 ataaatccgg ggcaatggtc tccttgtgat aaaagagctc tgtcattatt gactgtgctt
    10321 tttttggctg ctgttctttg agataagccg acgcaaccca atattgcgcc cagggcggaa
    10381 taatttgcct cgtatttttt aaacgctgat agtgagaaat aacgtcttta taacgatcgc
    10441 gagttaataa cgcgccaaga tgatcaacct gaatacgctg gtactgggca gtgcggtctg
    10501 agttatcgtg ccacagaatt tctaatgcag cgtattgggc gagggcgcga tcggcaatgg
    10561 cataacgttc actttcactg cgcgtaggca taaacgacag tctgatcagt tcggcatgaa
    10621 tatcagcgcg aatatctggc gttaaattgg catcgtcaat cgcggcagct agttgattat
    10681 tacgtaatgc ctgcacgtat tctgtgggat attgttgctt agatgcattt tcaggtaatg
    10741 actctgtcat cgcccgtaat tcatcctgat gacgccccgt cagtttatag atataggctt
    10801 ctgcgagtaa attggctttg tccggcgctc cagatttaag ctgcttaagt ttaaccagcg
    10861 cagtatcata gtgacccgca tctgccaggg ttaaaatttg tccccgttga taatccttat
    10921 tttgcggctc cagagagagc gccttttgcc acagtgtaag cgagttttgc cattgttgca
    10981 ggttacgata agcgacggcg acagctgcat aaccacgcgc tggtaattgc tgatgacggt
    11041 agcggctgta aacggtaatg acctgtttat cttgcccggc ccataaggca atttgtaacc
    11101 agtcagcaat ttggttatta ctgagtgttg atttttgtgc aaaccatgac aaagctggct
    11161 gagtatttcc tttgcgagct tcaataatca atgcatcata ggcgttatta acagcactct
    11221 tcgccgcggg actcgctgct aaaaatgcgg cagtaagaag tttcaaagcc catttggttt
    11281 tcgggcacct ttttctgcta cttgaataca tcctgtatta ctccatgtat tgccaaaatc
    11341 tctctctata tctaattaca ggtaactgaa aagaaagata tttttgcatc tcataatccg
    11401 ttattaaacg cggaagagac acgtgaattg ttgatgatga gaagaagaaa tgatgagcag
    11461 attgtccata taaaatcctt ttctcgcccg aaaatccatt ccaatgatga ggatcttcag
    11521 gaatacggca taaatcccaa tgcctttttc aaaataaatt aggattaaaa taattaaatc
    11581 agtaaattcc gatgcataat ttcggatttt tcgaaagtcc tgatgaaagg cagcgctact
    11641 gcactcacga atttaattgt tattcctaat gtatctatta attaatgctt tataaggatt
    11701 ttattataat cctttataag tatttgcaag cctgccttgc agcaaaaaat gtataaagca
    11761 ttaaagtgat aataattaca ttctgcaact atcatcatcg gtattgtttg tgggtggatt
    11821 ttcatattga caacagtaca gttcttatat ctattaataa taaaaaggga tctacaacct
    11881 acaggttggt gtagctttat ggaaaaagac tatttgggaa ttagtagtac tgtattagtg
    11941 agcttattgt ttgggcttgc attggttttg gttaatagtt ggttcaatca gccaaccgtt
    12001 gaggaagttg ttccacgatc aacctatctg atggttatga ttgctttatt ttttatcgat
    12061 actgttgcat ttatttttat gcagttgtat ttcatttatg accgtaggca attttcaaac
    12121 tgtatactta gtttggcttt tctaagttgt ttgatttact ttattaaaac cgtcattatc
    12181 attcagcaaa ttattgaggg gcgtttgaca agcagtgttg ttcaaaatga cattgcaatt
    12241 tattatttgt ttcgtcagat gagtttgtgc gtattaatat ttctggcatt ggtaaataaa
    12301 gttagtgaaa acacaaaaca gcgcaatttg ttttccaaaa aagtgacttt gtgcataact
    12361 ttgttttttg ttcttggggg gccgatagtt gctcacatat tttctagcca ttatgagagc
    12421 tacgatttac acattgccga gttaaccaat aaaaatggtc aggtggtttg gaaagcctca
    12481 tatgttacca taatgatttt catgtggtta accttgcttt cggttaatct atactttaat
    12541 ggtttacgct gtgatatctg gaacggggtg tcggtgattg ctttttgtgc cgtgctgtac
    12601 aatatctctc tgttatttat gagcaggtat agcgtgtcca tttggtatat tagtcgcact
    12661 attgaagtag tcagtaagtt aaccgttttg gtaattttta tgtgccatat attcagtgcg
    12721 ctacgagtaa caaaggacat tgcacatcgc gatcccttaa ccaatatatt taacagaaat
    12781 tattttttta atgaactgac ggttcaatca gcatcggcca aaaaaaaacc ttattgcgtc
    12841 atgattatgg atattgacca cttcaaaaaa gtcaacgaca cctggggaca tccggttggc
    12901 gatcaagtga taaagacagt ggtgaatatc attgggaaaa gcatacgacc agatgacctt
    12961 ttagcgcgcg ttggcggcga agagtttggc gtcttgctga cggagatcga tactgaatgt
    13021 gcgaaagctt tagcggaaag gattcgggaa aatgttgaac gtttaactgg cgataatcct
    13081 gaatacgcca taccacaaaa agtgacaatt agtattggcg ctgttgtaac gcagaaaaat
    13141 gagttaaacc ctaaggagat ctatcaactg gctgataacg cactttacga ggcgaaagag
    13201 accgggcgta ataaggtggt agtaaaagag gcagaaattc ttatcaaccg caaggatgat
    13261 gaatgagtct gtcgctagtt gtcgacctcc gcctggggct tctcatcccc cagtgtgtga
    13321 aatatacgaa aaaaagctcg cactttcgta cgagctcttc tttaaatatg gcggtgaggg
    13381 ggaccgcctg aaataaatct gacttaatcc gggagtgtta tataacttca ttataaacaa
    13441 tgcattacaa tttttgctgt ccgatgttat ctaatgtatt cttagtgaag tgagagacta
    13501 atggcatact ggatggcata ccagagtaac aggaattagc gtatggcagt attgacggat
    13561 actaaagcaa ggcatatcaa acctgatgac aaaccattgc cccatggggg aattacagga
    13621 ctgactcttc atccttcttc agtaaagggg cgggggaaat gggtttttcg ttatgtaagt
    13681 ccggtgacac aaaaaaggcg taatgctgga ttgggtactt accctgaggt cagtattgct
    13741 gaagctgcac gtactgcccg gataatgcga gagcaacttg ctgcaggtga tgatcctctg
    13801 gagattaaaa aggctgaagc tgaaaaagtt gttatcccaa catttgccga tgcagcccgg
    13861 cgtgtacatg cagaattgtc accgggatgg gaaaacccaa agcatgtcag gcagtggttg
    13921 tcgacgcttg aaaattacgt gtttcctcaa ctgggagcaa aaacgctgga tacgattacg
    13981 gctgcggacg tggcagagac actgcgtcca gtctggttga ccttatcaga aacggcaagc
    14041 cgggttaaac agcgcattca tgttgttatg cagtggggct gggcgcatgg tttttgtgtg
    14101 gcgaatcctg ttgatgtggt tgatcatttg cttccacagc aatcaagagg acgtgatgaa
    14161 caccagccgg caatgccctg gaggcagtta ccgctttttg tggcgaccag tgtgtataca
    14221 gatgaacctt ataatgttac ccgggcactg ttattaatgg tgatactgac agcaacccgc
    14281 tcgggcgaag caaggggaat gcgctgggct gaaattgatt ttcataagcg gatatggacg
    14341 atacccgcag aaagaatgaa agccaggata cagcatcgtg ttcctttatc ccgacaggcc
    14401 attcacgttc tggaaaatat acgtggtctg catgacgaac tggtgtttcc ttctcccaga
    14461 aagcagcaga tcctttcaga tatggtgttg acgagttttc tacgtaaaaa gaaggccatc
    14521 agtgatatac ccggacgagt ggctacagca catggttttc gttcaacatt cagggactgg
    14581 tgtagcgaac agggatattc gcgggatttg gcggaaaggg cgcttgccca cacgctgaaa
    14641 aataaggttg aggcggcata tcaccggact gatctgctgg atcagcgtat accgatgatg
    14701 caggcatggg cggattatgt gatgtctcag attatggaaa accagcgatg aaatcacatc
    14761 tcacagattt gcagatgtcg tctgtaatca cgaaaaatat acttattgag ttataatgct
    14821 tgtttgtctg atatttgcaa tgacataaat gtaaaaataa tacgtaatat tatagtttat
    14881 aaattgaatg ataaaaacaa tgccagtctg taagtgatgt ctggcattgt ttcagatatt
    14941 caggttggta actgaatgat aaaagacggg aactttaatt ttttgtagtt atgttccata
    15001 ttttagtctg ttcttcattt ctgtacttat tcagcttatg gtcccaacag accataaaca
    15061 ttccacgctc cattatcggg atgtttgcct catttgctaa ttcacaaggt gtaatcgcgt
    15121 ccccgatata aatgattttt tggccgattg aataatcaat tacgattttt ttctgttctg
    15181 caagtatatc aagtgcttca tcaagaacac atttattcct gagcgcatat ggaccatatt
    15241 gtagtatatg gcttttttga tattcgtatc cttttacccg gatgatattt tctctgagcc
    15301 atttatccag tttttctgca ggagatattt cccgaagtga ttccagtttg gatataagat
    15361 gctgaaaata aaaatcaacg agataaatag cactattgat actttctcgt gttattactg
    15421 taccatgtgg attgttctcc agctccagaa gtgcggctat tcgaacagcc tgttctcttt
    15481 tcctgacgat aatgtcttcg aaagtggtca tacgtgggtt ctgcctgact gcactcatat
    15541 tttctttgtg aaagatttcc agatcgtggg atgctgcgtt tgataatgtc atgcagatgc
    15601 gttgctcacc ttttgcacgc ctttcccggg acgctctcag agagtctctg atctgactgc
    15661 caaaactttt ctcatgaaaa caggttgcac attgctgcgt tacaacagga gtggcagtat
    15721 gcggttctgt cgtcattatt ttatttgctc cgggttcgca aaatagcacc cgggccagaa
    15781 agccattgcc ccggatctgg tcgccagtga gagtaaaaaa cttatcaaat attaccggtt
    15841 gagtgagtat aagcatagtg agacgggaat cctttacccg gaacccgggt ctggatgccc
    15901 gttctacggt gactggttgc ccactccata atgtgttgta cagcgccgat ttgcgttcga
    15961 aacgcttatc cagaatagtt cctccttcat cagacagcaa cagaagagaa ggtgactggc
    16021 tgagtttcaa agctatccct tctggtgtcg cgtcattcac gatgagaacc tgttgtacgg
    16081 gaggtacggg tttatttgct atacactttt caagcgctgc tcgtgcagca gcagcatttt
    16141 ccggggatct ttccatttct tttttatatc tccgttccag gattttttta tctgttgtcc
    16201 acatttccat catgcgatca tagttttttt tatcgtcgat atatgcctga tgtagttctt
    16261 cttctgtttc atgaacaggg gcgattaatt ttgtgcaggc agaggttttt ccactgcctg
    16321 atgctgatga aagacacata tatatctgaa gactggtacg ctgccctcca gaaagttcaa
    16381 cttcatgcgt atcctggacc gaggcagcct tgaccgtcag cataatagac agcagtattt
    16441 cacggcttat tcccgtttct tcatgcattt catcaagtaa tgcctggcac ctgtcaggca
    16501 gtttagttat gtcaagttca gggggggata attttgtcat ggcgtacagt tttccttatt
    16561 tatccttagt tacggatctg gttggtgttg tatgtaaagt taaccactca tcaatggcag
    16621 atgcccgcca gcctacagcc gaactcccaa gccggacagg ccggggaaag gtggcatcgt
    16681 agtattttga tgccggattc attttttcgt agatggtcga tcttgatata ccaaggagcc
    16741 gggtaagttc agccatacgc aggatgcgtg agggggtata aaaatgtccg gtctgggttg
    16801 acatattgtt ctccatgtga gttgaaggct atgtcattaa tttgctaggt taaggccaga
    16861 atgtcccctg tgataaaacg gaaaccaacc ggagatcaga gcagatgcgc atgtttacaa
    16921 atcttttgta tgatatttgt acagtttttg agttgtttaa ggaaggagag agtccgagag
    16981 ataagcggaa gagtactgat tttggagcgc atcaaagatt ctgggatcaa cgttataatg
    17041 agctcagtca cattattgat gctgaagggg tgtatagtct ggaacaacgg cgaattatat
    17101 tttcccggta tgaatatttt tattacatga tgaattctta ccctgtgtat tcaacattaa
    17161 agtccgaata tatccgtaac tattttc
//
LOCUS       DAZGQL010000106        17007 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.106, whole genome shotgun sequence.
ACCESSION   DAZGQL010000106 DAZGQL010000000
VERSION     DAZGQL010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17007
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.106"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(125..604)
                     /locus_tag="ACHKIR_004111"
     CDS             complement(125..604)
                     /locus_tag="ACHKIR_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9991782.1"
                     /translation="MQKYKQESLSAAMKESGLLIAKICACVIAGVVVIWGLVTGTETL
                     LTALKTMHEQYDNSMCYIVCATGWFFLVLSNWLWVTYSLYKARTGSTVGGGLQFMLLL
                     SLLAECFSFFVIFWSLSHLDITDIQLLVMGITTLMLFLSLNVKVVVIINHVASGNSD"
     gene            complement(716..1960)
                     /locus_tag="ACHKIR_004112"
     CDS             complement(716..1960)
                     /locus_tag="ACHKIR_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STY4528 family pathogenicity island replication
                     protein"
                     /protein_id="HFO9991783.1"
                     /translation="MTLPVDSLIAYTLEKMQERIEQKGRQQDGKIQSGLLFTGNVHDA
                     IPRRLLLDTRLSPLDKMAWMMIRLYAQNNEGAVFPTYDELQLQLASPGKGKASRETVS
                     RVLLMLRITGWLSLCKRVRDEKGRVRGNIYAQHDEPVTCRDAEILDPRYLDMVADACM
                     SRNRTISQTARDILCEIKNDPGMRHYHSHISQLEARIGSAQTPGQMAARQRVTEDRMQ
                     PGSETEPGVETQSSVLKMLSSDSELSEKQGQQILSSDSELSEKSDGYHRVRKPNHYVR
                     NITHSVIKNTYVLPEALTGQIREEEAIMLTSQLQALPEELAQGVLESLSGMLGSHSLQ
                     NPVGWLLAVLKRAREGNFYPPKQKQRVVVPASVLAGERGRATAQSVSSGKRTEPISAE
                     RLSGIVAGLRASLRRCSAMRSE"
     gene            complement(1978..2445)
                     /locus_tag="ACHKIR_004113"
     CDS             complement(1978..2445)
                     /locus_tag="ACHKIR_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9991784.1"
                     /translation="MAIAERTGISSRGDTYEIWLGANCYHYRSDGYAVLLKEGRNRAY
                     PCRVTSETIKSALAAIVELQQKLTAAEKKKECENHGFLLPAEMLIRCLEDQTGGRWIR
                     AHEPVMLMKRLISNLKKARPECTVPDEALAYLRRNCLVNMGDIPVTSRRMSDV"
     gene            complement(2449..2862)
                     /locus_tag="ACHKIR_004114"
     CDS             complement(2449..2862)
                     /locus_tag="ACHKIR_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XF1762 family protein"
                     /protein_id="HFO9991785.1"
                     /translation="MAEINFQVLREAEQLTGVATAGRPVARHFDDGLTLEVNRTCTTG
                     ERNANSALYGAVWRAAKAMGYRRCITYTRADESGASLRAAGFVRVKELPSGKSWAESS
                     VALKSTRDPVGSGGVPRVLREIRRMSTTVIHIKGE"
     gene            complement(2877..3485)
                     /locus_tag="ACHKIR_004115"
     CDS             complement(2877..3485)
                     /locus_tag="ACHKIR_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2857 domain-containing protein"
                     /protein_id="HFO9991786.1"
                     /translation="MNNSLSQATNGLLMQLVMDLKSGYLRRCESLGLAREEMQMLQGL
                     TIEEIHYLSNSEVSVIRLDINHENLLRMLQQARTEQKRLQRIDRALALGGSIELMAFY
                     FGLSSVDVAARRRIAGIDVRPGRGITLSDEESAELWRLWQKSGITDVESADGLDVMML
                     AAEQMNIPLTAVWHAVRGWHQGTAGFNDVSASRRKLNREKAE"
     gene            complement(3482..4207)
                     /locus_tag="ACHKIR_004116"
     CDS             complement(3482..4207)
                     /locus_tag="ACHKIR_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2786 domain-containing protein"
                     /protein_id="HFO9991787.1"
                     /translation="MSDMSHQERLIRRVRKLLELSRNNSNAHEAGLALARAQQLMAKH
                     GITEQDAGLSSVQTSSSQGAPSDAEKVPAWMFRLVMVVTQTFGCRAYYDWRAVPGDRY
                     RRCVTFYGFSERPQVAAYAFDVLCRHLREATDAYLRTQSRRLKLSTRRARAAQFRDGW
                     VQGIRQVVMPFSVTEEESRLMRLWMEQQDMVTVDSREMKACRGGDLARWQGYEAGKNA
                     SLHHGVGESARQVVPGLMIREAS"
     gene            complement(4200..5933)
                     /locus_tag="ACHKIR_004117"
     CDS             complement(4200..5933)
                     /locus_tag="ACHKIR_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB family protein"
                     /protein_id="HFO9991788.1"
                     /translation="MAHKPLDLGAAIMQPGRQTTGAGNVVAMGETPMVLTLDQLRPNP
                     DNPRTTRNPRYDDIKNSIHARGLDTVPKVTRMPDSEPDVYIFSDGGNTRYQILTELWK
                     ETGDPRFYRIHVLFKPWPGRLQCVIGHLAENEVRGELTFIEKAQGVHKARQIHEEQLG
                     RTVSLRELADLLTHEGLPIHNSTISRMEDALKYLYPWIPDLMESGLGRPQVTALLALR
                     QDAERVWGQFAVAADTDAEFDRVFGESCRKFNSPELWSLEMFRDELIGDLLQALPHPS
                     LDYDRWLLELDPKERNRRHHFGEPEPVVVPPSVRGSSTVQPGAPVSGIRSEVPECQPD
                     TDTEAMPESTVSIFPGTERKQHGITRPLKAVSDADTERTEKCSRPSQEESAGRGDSGA
                     SSAENALRPEIQPDMYGGKPVFSGDVMEDGAAGAVLASVMTGDTAPGTTDALPASVTH
                     FAVGRDDIEHLQNEAFRLAWELAESAGCAEEIAMDRESDLSAGFGAAVEKCSPVVAFL
                     AGLAGDAPRTVLPVSLKDLLTGGSAPEAWPLLDDDHAVKLLRLLSVLRRLRALQRSVL
                     PDDDEGDENDE"
     gene            complement(5933..7300)
                     /gene="dnaB-PI"
                     /locus_tag="ACHKIR_004118"
     CDS             complement(5933..7300)
                     /gene="dnaB-PI"
                     /locus_tag="ACHKIR_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPI-7-type island replicative DNA helicase"
                     /protein_id="HFO9991789.1"
                     /translation="MAEMSVLCAYEAEQAVLGGLMLENERWDEVVLMLSPDDFYVAQH
                     RAIFRAMSELAVNGQPLDLITLSEHLEHQGRLDVLGGFAYLAELSKNTPSAANIMAYA
                     GIVVEKSVLRQLQATGNTLIADVAAPNATSRDVLDAAERRLFALAQNRTLRERTEASL
                     SSALDTVLQQLESVLGSGGVTGTPTGFDCLDEMTCGLQPGDLVLLAARPSMGKTSLAL
                     SMCLSAALRRTEESVLVFSIEMPKEQLILRMLSMLGRVELNHLRSGNMDDEDWLRVSS
                     AVGMALGDGGMGSLGERIIIDDCCEQTPSSLRAGARRYTRLYGKPSLIMVDYLQLIRA
                     PELENRTQEIAEISRSLKALGKELGCPVLALSQLNRQVEQRADKRPNNGDLRDSGALE
                     QDADLIMFIYRDEVYNPDSLAKGEAEIIVSKQRQGPTGTVRVRFDGRYTLFTPSENDG
                     PGGYA"
     gene            complement(7648..8550)
                     /locus_tag="ACHKIR_004119"
     CDS             complement(7648..8550)
                     /locus_tag="ACHKIR_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9991790.1"
                     /translation="MSSSVKYSPVPAVLTRPELLAELNLLMTRIASGCRVGLPASGRP
                     VIAVHGGYVSYSSPRTGHVFQDGSRPETVMTVSNRERMCEWFSQVWLPLHLAALEGDE
                     NAFFSAEVNMVRRELQSVPGNSVNCMCWASLAALAFDAEERVERQKGNNENIATYDES
                     IATYAFRAACTVSRCLSRNASGGIPRSYPEWKAILFPRDGADIFPDGWLENMTRRAER
                     VFTSAKRQCGMLSASQAALFSQQTRQCASEQCTGRIQAGRHHSGSHPDVSVYTERCAQ
                     GMSLSPVHSARQCPVQPTDSAETE"
     gene            complement(8786..8995)
                     /locus_tag="ACHKIR_004120"
     CDS             complement(8786..8995)
                     /locus_tag="ACHKIR_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9991791.1"
                     /translation="MCVGCGYSGVRDGAGKNALVWVKVNPESGLGLCSGCDSFLLQER
                     QRRQRHDQLFSCGLSCKQRKTEGAV"
     gene            complement(9328..10215)
                     /locus_tag="ACHKIR_004121"
     CDS             complement(9328..10215)
                     /locus_tag="ACHKIR_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO9991792.1"
                     /translation="MKILPVISTKGGEGKSTQSANLAGFLADAGLRTLLIDGDHAQPT
                     ASNIFPLTYEAPAGLFELLMRTADLSHPDNIISRTAVRGLDLIVSNDPHEQLKTAMLH
                     APDGRLRLRNVLQHPLFQIYDVIVVDSQGARSVMLEMIVLSATESVVGMVNPVLPDVR
                     EFIRGTVNVMENLMPYRELGIPLPKVRTLVNCMDYTALARQTLAELTGIINAGRYSKV
                     LPEGAVTLLSTQIYDLNIYKQGHAAGQPVHRLEKVSARCSDSALVTMHSLACELLPEW
                     REFFDALLENGGKSLWAEN"
     gene            10531..11715
                     /locus_tag="ACHKIR_004122"
     CDS             10531..11715
                     /locus_tag="ACHKIR_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO9991793.1"
                     /translation="MALTARQVETARPKEKDYKLSDERGLYLLVKTTGARYWRLKYRI
                     AGKEKKLALGVYPDVSLAEARIKRDDARKIISEGGDPGEKKRKEKLTQKISATNTFHA
                     LATEWHQHKSLSWSESYARSVLEALDKDIFPYLGKRSVTDILPLEMLEILRRIEKRGS
                     LEKLRKVRQYCNQIFRYAIATGRATVNPASELTSTLAAPKAAHFPHLRADELPVFLRK
                     LAEYHGSPVTRMATNLLLLTGLRTIELRSAEWSEIDFDNALWTIPESRMKMRRKHVVP
                     LSRQVTDILQQLKTFSGQYRLVFPGRCDINKPMSEASINMVLKRIGYDGRATGHGFRH
                     TMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYIDGRREMMQWYADYIDSLSEQ
                     VS"
     gene            12044..12625
                     /locus_tag="ACHKIR_004123"
                     /pseudo
     CDS             12044..12625
                     /locus_tag="ACHKIR_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
     gene            12712..12990
                     /locus_tag="ACHKIR_004124"
                     /pseudo
     CDS             12712..12990
                     /locus_tag="ACHKIR_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(13258..14262)
                     /locus_tag="ACHKIR_004125"
     CDS             complement(13258..14262)
                     /locus_tag="ACHKIR_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013315785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFO9991794.1"
                     /translation="MQNITLIGIDLGKHSFHIHCQDKSGKVLLRKKFTRTKLIEFLAG
                     CASSTVVMEACAGAHFMARRIADLGHEAKLISPQFVRPFVKSNKNDFVDAEAICEAAS
                     RPSMRFVQPRTEAQQAMRALHRVRESLVSDKVKTTNQMHAFLLEFGISMPTGVAVVKR
                     LSTVLAENELPPYLAQLLMRLHAHYIYLVGQITELEIALEQELSRDEVGQRLQTIPGI
                     GPITASVLSSQLGDGKQYSCSRDFAASTGLVPRQYSTGGKNTLLGISKRGDKNLRRLL
                     VQCARSFIQRIENQSGRLADWVRALLARKHSSVVCCALANKLARIAWAITTQQTVFER
                     "
     gene            14311..15266
                     /locus_tag="ACHKIR_004126"
                     /pseudo
     CDS             14311..15266
                     /locus_tag="ACHKIR_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            15306..15785
                     /locus_tag="ACHKIR_004127"
                     /pseudo
     CDS             15306..15785
                     /locus_tag="ACHKIR_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
     gene            15928..16157
                     /locus_tag="ACHKIR_004128"
                     /pseudo
     CDS             15928..16157
                     /locus_tag="ACHKIR_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327330.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(16144..>17007)
                     /locus_tag="ACHKIR_004129"
     CDS             complement(16144..>17007)
                     /locus_tag="ACHKIR_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFO9991795.1"
                     /translation="VPTTPWLNTAPGLFIDGYTSAAASTVPSLSRTLIYDYEQNPDSG
                     NNVVALAAKAGYSTWWISNQGKLGEHDTRISVIASDAEHTVFLKKGSFASRKTDDMLL
                     LQETERALADKSSPKVIFLHMIGSHPNPCDRLNSWPNHYLEQYPRKIACYLASISKLD
                     NFLGQLDGILRRHSRHFAMLYFSDHGLSVSDSANPVHHDGHVQGGYSVPLIITASDIT
                     SHQSVSRKISARHFAGIFQWLTGIRTENIPPFNPLTDEDNEPVMVFNGERNVPADSLK
                     PQPLILPVRGK"
BASE COUNT         4203 a         4714 c         4018 g         4072 t
ORIGIN      
        1 cagcactgtt cactttttaa cctcttctgc cactggcagc actgttcact ttttgacctc
       61 tgctgccact ggcagcactg ctcatttttt aacctctgtt gccactggca acaccgctca
      121 ttttttaatc actgttgcca ctggcaacat ggttaatgat gactactacc ttaacgttca
      181 gagacaaaaa cagcattaat gtcgttatcc ccatcacgag caactgaatg tcggttatat
      241 ccagatgaga caatgaccag aaaatcacaa aaaatgaaaa gcattctgcc agcagactca
      301 ggaggagcat aaattgcagg ccccctccga ctgtactgcc tgtacgcgct ttataaagac
      361 tgtatgtgac ccacagccag ttacttaaca ccaggaaaaa ccaccctgtg gcacatacga
      421 tataacacat actgttatcg tactgttcgt gcattgtttt cagcgcagtg agcagggtct
      481 ctgtgcctgt caccagcccc cagataacca ccactccggc aatcacgcag gcacaaattt
      541 tagcgatcaa caggccactt tctttcatgg ctgcactcag actttcctgt ttgtatttct
      601 gcatgctatt gaccaccttg tttcacctga ccagttcccg attctgtttt ctttttaacc
      661 agtcgctggc ggcactggtt gatctttgtt cccgcgaaca tagcccgtat tgcaatcact
      721 cagatctcat cgcagaacat ctgcgcagcg aggcccgtag tccagccaca atgccactca
      781 gacgttctgc tgaaatcggt tccgtacgtt tccccgatga aactgattgc gcagtagctc
      841 gccctctttc tcctgccagt acggaggctg gcacaacaac acgttgtttc tgcttcggcg
      901 gataaaaatt tccctcccgg gctctcttca gcactgcaag caaccagccc acaggattct
      961 gcagcgagtg gctgcccagc atccctgaca gactttccag aaccccctgc gccagttctt
     1021 ccggcagcgc ctgcaactga ctggtcagca taatggcctc ctcctccctg atttgccctg
     1081 tcagcgcttc aggcaatacg tacgtgtttt taatcacact gtgtgtgata ttacgtacgt
     1141 aatggttcgg tttccgaact ctgtgataac catctgattt ttctgatagt tcggaatccg
     1201 aactcagtat ttgttgcccc tgtttttcac tgagttcgga atccgaactc agcattttca
     1261 ataccgaact ctgtgtttct acccccggtt cggtttccga acccggctgc attctgtcct
     1321 cagtcactcg ctgacgggcc gccatctgtc ccggtgtctg ggcactccct atccgggctt
     1381 ccagttgact gatatggctg tgataatgac gcattcccgg atcatttttt atttcgcaca
     1441 gaatatcccg ggccgtctga cttatggtcc ggttccggct catacaggca tctgccacca
     1501 tatccagata acgtggatcc agtatttcag catcccggca cgtcaccggt tcatcgtgct
     1561 gggcataaat attgccccgt acccgccctt tctcatcacg cacccgtttg cacagactca
     1621 gccagcccgt gatccgcagc atcagcagca cccggctgac agtctccctg gaggcttttc
     1681 ctttccccgg ggaagccagc tgcagttgca gttcgtcata tgtggggaaa actgcacctt
     1741 cattattctg tgcatacagc cgaatcatca tccacgccat cttatccagc ggagaaagtc
     1801 gagtatccag aagcagtcga cgcggaatgg catcatgcac atttccggta aacagcagtc
     1861 cgctttgtat ttttccgtcc tgttgtcggc ctttttgctc tatccgctct tgcatttttt
     1921 ccagcgtgta cgcaatcagg ctgtctacgg gtaatgtcat tacacttttc ctttttgtta
     1981 tacgtcagac atccgtcggc tggtcacggg tatatcgccc atattcacca gacagttacg
     2041 ccggagatat gccagcgctt catcaggaac ggtacattct ggtctggctt ttttcaggtt
     2101 actgattaat cgcttcatca gcattactgg ttcatgtgca cggatccacc gtccccctgt
     2161 ttgatcttcc agacagcgaa ttaacatctc agctggcaaa agaaacccgt ggttctcaca
     2221 ctcctttttc ttctctgccg cagttaattt ctgttgcaat tcaacaattg ctgccagcgc
     2281 agatttaatc gtttcactcg tcacccgaca tggataagca cgattcctgc cttctttcag
     2341 aagcactgca taaccatcag agcggtagtg ataacagttt gctcccaacc agatttcgta
     2401 agtatcgccg cgcgaggaga tacccgttct ctctgcaata gccatatctc attccccttt
     2461 gatgtgaatg acagtagtgc tcattctcct gatttcccgg agcacacgag gaacaccacc
     2521 gcttccaacc ggatcgcgtg tactcttcag ggcgacactt gattccgccc agctttttcc
     2581 tgaaggaagt tctttcacac gaacaaaacc agctgcccga agagaggctc cggattcatc
     2641 agccctggtg tatgtaatgc aacggcgata tcccatagct tttgccgccc tccagacagc
     2701 gccataaagt gcgctgttag cgttacgttc accagtagta caggtacgat tgacctccag
     2761 tgtcagccca tcgtcaaagt ggcgtgcaac aggtctgccg gctgtcgcca cacctgtcaa
     2821 ttgttcagcc tcccgcagta cctgaaagtt aatttcggcc atgtcatttg tccttgtcat
     2881 tctgcttttt cccggttcag tttccggcga ctggccgata catcattaaa ccccgcagtt
     2941 ccctgatgcc atcccctcac ggcatgccat accgccgtca gcgggatatt catttgctca
     3001 gctgccagca tcatgacatc cagtccgtcc gcactctcca catccgtgat gccgctcttc
     3061 tgccataaac gccacagctc tgcactttcc tcatcactca gtgtgatgcc acgtcccggg
     3121 cgaacatcaa taccggcaat gcgacggcgc gccgccacat ccacactgga aagtccaaag
     3181 taaaacgcca tcagttcaat ggagcctccc agcgccagtg cccggtcaat gcgctgcaga
     3241 cgtttctgct ccgtccgggc ctgctgcagc atacgcagca ggttttcatg attaatgtcc
     3301 agccggataa ccgacacttc agagtttgac aggtaatgaa tttcctcaat ggtcagtccc
     3361 tgcagcatct gcatttcctc ccgggccagc cccagagatt cgcagcgacg caggtagccg
     3421 ctcttgaggt ccatcaccag ttgcatcagc agaccgttgg ttgcctgaga caagctgtta
     3481 ttcatgacgc ctcccgtatc atcagacccg gcaccacctg tcgggcactc tcccccacac
     3541 catgatgcag tgaggcattt ttcccggcct catacccctg ccagcgggcc aggtcaccac
     3601 cgcgacaggc tttcatctca cgggagtcca ccgtcaccat atcctgctgc tccatccaga
     3661 gacgcatcag ccgactttcc tcttctgtca cactgaatgg catcaccacc tgacgaatcc
     3721 cctgaaccca gccatcacga aactgagccg cccgggcacg acgggtgctc agtttcagtc
     3781 tccggctctg tgtacgaaga tacgcatccg ttgcttccct tagatgacgg cacagaacat
     3841 caaacgcata agccgccacc tgaggacgtt cactgaaacc gtaaaacgtc acacaccggc
     3901 gataccggtc tccgggcacc gcccgccagt cgtaatacgc ccggcagcca aatgtctgtg
     3961 tcaccaccat gaccagacga aacatccacg ccggaacttt ttcagcatca gaaggtgccc
     4021 cctgtgatga agaggtctgc accgaagaaa gtccggcatc ctgctccgtg ataccgtgtt
     4081 tcgccatcag ctgttgcgcc cttgcaagcg ccagcccggc ctcatgggca ttgctgttat
     4141 tacggctcag ctccagcagt ttcctgaccc gcctgatcag acgttcctga tgcgacatat
     4201 cactcatcat tctcatcccc ctcatcgtcg tcgggtaaca cactgcgctg cagggctcgc
     4261 agacggcgca gcacactcag aagacgcagc agcttcacgg catggtcgtc atccagcagt
     4321 ggccacgctt ccggtgcact gccccctgta agcaggtctt tcagactcac ggggaggact
     4381 gtccgcggtg catcaccggc cagccccgcc agaaacgcca ctacaggaga acatttctcc
     4441 actgctgccc cgaaaccggc agacaggtcg ctttccctgt ccatggcaat ctcttcagca
     4501 caaccggctg attcagccag ttcccaggcc agacggaacg cttcattctg cagatgttcg
     4561 atatcatccc gccccacagc aaagtgcgtc accgacgcgg gaagagcatc agtggttccc
     4621 ggggcagtat ctcctgtcat aactgatgcc agtactgctc cggcagcacc atcttccata
     4681 acatcaccac tgaagactgg cttaccaccg tacatatccg gctgtatctc aggacgcagg
     4741 gcattttcag cagaagacgc cccactgtcc cccctgcctg cgctctcttc ctgagaaggt
     4801 cgactgcatt tttcagtgcg ctctgtgtca gcatcagaaa cggctttcag tggccgggta
     4861 ataccatgtt gcttgcgctc agtaccgggg aatatggaga ctgtgctttc cggcatcgcc
     4921 tctgtatccg tatccggttg acactccggt acttcagacc ggatcccgga taccggtgcc
     4981 ccgggttgta ccgtactgct gccacggact gaaggaggca ccaccaccgg ttccggctca
     5041 ccaaaatgat gacgacggtt tcgctctttc ggatccagtt ccagcaacca ccggtcatag
     5101 tccagggagg gatgtggcag ggcctgcaac aaatctccga taagctcatc gcggaacatc
     5161 tccagtgacc acagttccgg cgaattaaac ttccggcagc tttccccgaa cacgcggtca
     5221 aattccgcat ccgtatcggc tgcaacagca aactgccccc atacacgttc tgcgtcctga
     5281 cgaagtgcga ggagtgctgt aacctgtgga cgccccagtc ctgactccat caggtcgggg
     5341 atccaggggt acagatactt cagggcatct tccattctgc tgatcgtgga gttatgtatc
     5401 ggcaaccctt catgggttaa caaatcagcc agttcacgaa gagacactgt tctgcccagt
     5461 tgttcctcat ggatttgtct tgccttgtgg accccctgcg ccttctcaat aaaagtcagt
     5521 tcaccgcgaa cttcattttc agccagatga ccaatcacac actggagccg acccggccag
     5581 ggtttaaaca gcacatgaat acggtaaaaa cgcggatccc ctgtttcttt ccagagttcc
     5641 gtcagtatct ggtaacgggt gtttcccccg tcactgaaga tatacacatc cggctcactg
     5701 tccggcatac gggtgacttt gggcaccgta tccagcccgc gggcatgaat ggaatttttg
     5761 atatcgtcat aacgcggatt gcgcgtggtg cgcgggttat ccggattcgg acgtaactgg
     5821 tccagcgtca gaaccatggg cgtttccccc attgccacca cattgccggc accagtggtc
     5881 tgacgaccgg gctgcataat agccgccccg agatccagtg gtttatgtgc catcacgcat
     5941 atcctcccgg cccgtcattc tcagagggcg taaacagtgt gtaacgaccg tcgaacctga
     6001 cccgtaccgt acccgtgggc ccctgacgct gcttgctgac aatgatttct gcctccccct
     6061 ttgccagtga gtcggggtta tagacctcgt cgcggtaaat aaacataatc aggtcagcat
     6121 cctgctccag cgccccggaa tcacgcaggt caccgttatt ggggcgttta tcagcacgct
     6181 gctccacctg ccggttaagc tgggagaggg ccagcaccgg gcagcccagc tccttcccca
     6241 gcgctttcag ggaacgggaa atttcagcaa tttcctgtgt gcggttttcc agctccggcg
     6301 cacgaatcag ttgcagataa tccaccataa tcagggaggg tttaccgtac agacgtgtat
     6361 aacgacgggc gcctgcacgc agtgatgaag gcgtctgctc acagcagtca tcaattatta
     6421 tgcgctcacc cagagagccc attcctccgt caccaagcgc cataccgacc gcagatgaca
     6481 ccctcagcca gtcctcatca tccatgttgc cactgcgcaa atgattcagt tccacacgcc
     6541 ccagcatgga aagcatgcgc agaatcagtt gctccttcgg catctcaatg ctgaatacca
     6601 gaacgctctc ttccgttcgc cgcagtgcgg cgctgagaca catggatagc gccagagagg
     6661 ttttccccat tgacggacgg gcagccagca gaaccaggtc accaggctgc aggccacagg
     6721 tcatttcatc cagacagtca aatcccgtgg gggtaccggt aacaccaccg gagccgagca
     6781 cactttccag ttgctgcaga acggtatcaa gcgcggatga aagagaggct tccgtgcgtt
     6841 cccggagcgt cctgttctgc gcaagtgcaa acagacgccg ctctgcagca tccagcacat
     6901 cccgtgatgt ggcgttcggt gccgccacat cagcaatcag ggtgtttccg gtggcctgta
     6961 actgacgcag aacgcttttc tccaccacaa tgcctgcata ggccatgatg ttggccgcag
     7021 aaggtgtgtt cttgctgagc tcggccagat aagcaaagcc gcccagtacg tccagcctgc
     7081 cctgatgttc caggtgctct gacagtgtaa tcaggtccag tggctggcca ttcaccgcca
     7141 gttcagacat ggcacggaaa atagcccggt gctgagccac ataaaaatcg tcaggactca
     7201 gcatcagcac cacttcatcc cagcgttcat tctccagcat cagaccgccc agaacggcct
     7261 gctctgcttc gtatgcgcag agaacagaca tttcagccat ggcgccccct ccccctctga
     7321 cagccggaaa cctggctacg tgaaaaaagg aaaaagggtg ccggacgtac agctgtcggg
     7381 aataaaatcc cggtaactgt ctgtacgttt tcagaccggt gagtttgccg cccccggtct
     7441 gaaggtacgg acagcattcg ttcattcata acaagttgtt ttaaaggaaa aagtttatga
     7501 atacagaaaa acaactgccg tccggcattg aatctgaagt gaaatacacc ctgcagttct
     7561 ggctgctgag taacggaaag gtcatgacgg aaaaagacgg gctctgtaac ccctttcccc
     7621 tgaactgtat tgaatcggct catcctttta ctccgtttca gcagaatcag tgggttgaac
     7681 tggacactgt cgggccgaat ggaccggtga aagcgacatt ccttgtgcac agcgttctgt
     7741 ataaactgaa acgtcagggt ggctccctga atgatgccgg ccggcctgaa tacggccggt
     7801 gcattgctca ctggcacact gacgtgtttg ttgagaaaaa agcgctgcct gactggctga
     7861 gagcattcca cactgacgtt tcgcactcgt aaaaacacgt tcagcccgac gggtcatatt
     7921 ttccagccac ccgtcgggga aaatgtcagc accatcacgg ggaaacaaaa tcgctttcca
     7981 ttcagggtat gagcgcggaa tacctccgga agcattccgg ctcagacagc gactcacagt
     8041 gcaggcagca cggaatgcat atgttgcgat gctctcatca tatgttgcga tgttctcatt
     8101 attgcctttc tgccgttcca cccgctcttc agcatcaaat gccagtgcag caaggcttgc
     8161 ccagcacatg cagttcactg aattccccgg aacgctctgc agctcccggc gcaccatgtt
     8221 gacctcagca ctgaaaaaag cgttctcatc gccttcaagg gcagccagat gcagcggaag
     8281 ccagacctga ctgaaccatt cgcacatccg ttcccggtta ctgacggtca tcactgtctc
     8341 cgggcgggaa ccgtcctgaa aaacatgtcc ggtgcgtgga gaactgtatg aaacataacc
     8401 accgtgcacc gcaatgaccg gacgacctga cgccggaagc cccacacgac acccactggc
     8461 aatccgggtc atcagcaggt tcagttcagc cagtaattca gggcgggtta agaccgccgg
     8521 aaccggagaa tatttcactg aggaagacat actttcacct ccatatctct cagcccgcca
     8581 ggtgcgtcac ttccccgcag agaaccggaa acctggctat atgaaaaaag ggaaaagggt
     8641 gccggacgaa cagttgtcgg gaataaaatc ccggtaactg tctgtacgtt ttcagaccgg
     8701 taagtttggc gaccctggtc agaaggtacg gacagcatct ttgcgctcat aactgtaccg
     8761 accgggatat gcattacaaa gactgtcata ccgccccctc cgtctttcgc tgtttgcagg
     8821 aaagaccaca tgaaaaaagc tggtcatgcc gctgacgacg ctggcgttcc tgcaaaagaa
     8881 aggagtcaca accggaacac agccccagac cactctcagg attcaccttc acccagacca
     8941 gagcattctt accggcacca tcccgtaccc cggaataacc acagcccacg cacaccgtcc
     9001 acttctgcag tacgatgagt gcagtttcga gtgatgcgcg gagacgccgg gaggcaataa
     9061 ggagcaacgg ctcatggtca taaaaaaccc cgccgttacc ccctccggat tcaagcagcg
     9121 ggagtcctgc aggctcccgg ggacctgttg acatttcccg taattcgttt tcattccaca
     9181 tattgtggaa agttatccgg ttcactgaac aacgccattc ctccgccaga cgctgataca
     9241 aatgcttttt cgcttctgcg cggtcagatg catcaacaaa cacatgcact tcctcgtgtc
     9301 cgggtgctga agccacggca cgccatatca gttttctgcc cacaggcttt tccctccgtt
     9361 ttccagcaga gcatcaaaaa actcacgcca ctccggcaac agttcacagg ccagactgtg
     9421 catggtgacc agcgcagaat cgctgcaacg ggcagacact ttttccagac gatgcaccgg
     9481 ctgaccggct gcgtgcccct gtttataaat attcaggtcg taaatctgtg ttgaaagaag
     9541 ggtgaccgca ccttcaggca gcactttgct gtagcgaccg gcgttgataa tgcctgtcag
     9601 ctctgccagt gtctgtctgg ccagcgccgt ataatccatg cagttaacca gggtgcggac
     9661 tttcggcagg ggaataccca gttcacggta gggcataagg ttttccatga cattgaccgt
     9721 gccgcggatg aactcacgca catccggcag aaccggattc accatcccca ccacagactc
     9781 cgtggcggac agcacaatca tttccagcat gacagaacgt gcgccctgtg aatccaccac
     9841 aatgacgtca tagatctgaa aaagcggatg ctgaagcaca ttacgcagac gaaggcgacc
     9901 atccggcgca tgaagcatgg ccgttttcag ttgctcatgg gggtcgttgg agacgatgag
     9961 gtcaagcccc ctgacagccg tacgggaaat gatgttgtca ggatgactga ggtcagccgt
    10021 gcgcatcagt aattcaaaaa gaccggccgg cgcttcatac gtcagcggaa agatattgct
    10081 tgccgtgggc tgtgcatggt ctccgtctat cagcagagta cggaggccgg catctgccag
    10141 aaaaccggca agattagcag actgggttga ttttccttcg ccacctttag tggatataac
    10201 cggtaaaatt ttcatatagc acactcttta aagtatgcaa ccaatcactt acactacgga
    10261 aaccagccag aatattcact tcactacctg aatactcgca acacggggat tgaaaatccc
    10321 cgtgtccttg gttcgattcc gagtccgggc accactttca cttccagatt catctcccga
    10381 actccacgaa actcaagtat aatcacatcg ttacaagcaa tctgaacatc ctgaactcca
    10441 ccgaactcta ctgaactctt tctcaatcaa gctttcattg ggggcaccgg tgggggctca
    10501 ttatgttcag tcagtttcag gagcccccat atggcactta cagccagaca ggtcgaaaca
    10561 gccagaccta aagaaaaaga ctataaactc tctgacgaac gtggtttata tctgctggta
    10621 aaaaccacgg gagcccgcta ctggcggctt aaataccgga tagcaggaaa agagaaaaaa
    10681 ctggccctcg gcgtctatcc cgacgtctcc cttgctgagg ccagaatcaa acgtgacgat
    10741 gcccgaaaaa tcatctccga aggtggtgac ccgggcgaaa agaagcgaaa ggaaaaactc
    10801 actcagaaaa tctctgccac caatacgttc catgccctcg ctacggaatg gcaccagcat
    10861 aaatctttgt catggtctga aagttacgcc agaagcgtac tggaagcgct ggataaagat
    10921 attttcccgt atctgggcaa acgaagcgtt acggatatcc tcccgctgga aatgctggaa
    10981 attctgcgcc gcatagaaaa acgtggctcg ctggaaaaac tgcgtaaggt acgtcaatac
    11041 tgtaatcaga tttttcgtta tgccatcgcc accggacgag ccactgtcaa tccggcatct
    11101 gaactgacca gtacgctggc ggcgccaaaa gctgcacact tcccccacct gagagcagat
    11161 gagctccctg tttttctcag gaagctcgct gaatatcatg gcagtcctgt tacccgcatg
    11221 gcgacaaatc tgctgcttct gacaggcctc agaacgattg agctacggtc cgctgaatgg
    11281 tcagaaattg attttgataa tgccctgtgg acaatccctg aaagccgcat gaaaatgcga
    11341 cgtaaacatg tcgtaccact gtcacgacag gtcactgaca ttctgcaaca actcaaaact
    11401 ttctccggac aataccggct ggttttcccg ggacgttgtg atatcaacaa gccaatgagc
    11461 gaagccagca tcaatatggt gctcaaacgt atcggttacg atggcagagc aaccggtcat
    11521 ggttttcgtc acaccatgag taccattctg cacgaacagg ggtttaattc tgcctggatt
    11581 gaaatgcagt tagctcatgt ggataaaaat tccatcaggg gtacctataa tcatgccctg
    11641 tatattgatg gtcgccgtga aatgatgcag tggtatgctg actacattga ttcgctttct
    11701 gagcaggtaa gttaagctga gaacacgtta ttctgatttt cactcacttc acgattattc
    11761 ctccggcctg caggttcgga ggattcttat ttttatgttt tattttcaga taaagttttt
    11821 gacaaaacgt ttattctgca tcagcaaaat tatcacaaaa caacgcaatc acagacaaat
    11881 taacattttt cagacgggta tattgagtgc ccacaatgta agacaggcag gcatccggtg
    11941 acatggtaaa atattaatcc agggcaaaaa caaggcttga tacacaacat catgactttt
    12001 atgctctcag actatgccac cacagatttt acaggctcag aaaatgaatg acagattacg
    12061 ctttgaagtg aacgataacc agggctgttt cgtgttcccg gacgcctggt ttggctcgtt
    12121 gctggatgag tttgacgaac taattgatgc atacgacaga gatgaaatca gcgaaacaag
    12181 ttatatcaac aaactcagac gccttgcccg acaggagcct gactttattg atgtacacgc
    12241 ttacctggca tgcgcatttc tggagcagaa tgccccgcgt aaggcactga acgctgctct
    12301 taaaggactg gcagcaggta atcgtctcat cccggaaagt ttcagtggtg aaatcatctg
    12361 gatgcatccc gaaaaccggc cttatctgcg cgcgctttat gcagcaattc tcgcaaatgt
    12421 gcacttacag cgtcatcagg atgcagctct gctcactgat aaaatactgg catacaatcc
    12481 tgaagacaat cagggagcac gctggctact gggaccggaa ctgttacgta ctggcgacca
    12541 tgaacaggca cgccatgtac tgaaagcgca tgcagatgaa ttttcacctt actggtatga
    12601 actcggcctt ctgcattttc tgaaccggcc ccttgaaacg ccagacagtg gatgtatctt
    12661 aaaataagag ataggcttgg atatatgtct aacactagtt ctaattttga gatgactgga
    12721 attttgctgg ggcaggagct tcgtaaacgt aaaactcctc aggaaaaaat cgccattatt
    12781 cagcagacaa tggaacccgg tatgacggtg tcccatgtgg cgcgtttgca tggtattcag
    12841 cccagcctgt tgtttaagtg gaaaaaacaa tatctggaag gaagtctcac tgctgttgct
    12901 gccggagagg atgtcgttcc tgcctcagaa ctggctgccg ctataaaaca aataaaagag
    12961 ttacagcgct tgctgggtaa gaaatctatg gtccccacca tttttgcaac actgattttg
    13021 attgatgttg gcttgcctaa atctatccgg cgtcattatg ggcatggatg cccgcgcctc
    13081 gatgagttcc gcacctgatg agcccaaaaa accatccggc ttcagtgagc cattttcttg
    13141 ctgaggcttt tcatcaggcc gatatgccgt tcatgtcatc aataatcagt cttcgcaaaa
    13201 ccagattggg aactacttta attcatgatt aacctgccag ccagactggc gccatcgtta
    13261 cctttcgaac acagtctgct gtgttgtgat tgcccacgct atccgggcta atttgttggc
    13321 cagtgcgcaa catactacgc tggagtgttt tcttgccaac aacgccctga cccaatccgc
    13381 caaccttcct gactgattct cgattctctg aataaaggat cttgcgcact gaacgagtaa
    13441 ccgtcgcaga tttttatctc cacgtttact tatccctaaa agtgtatttt taccacctgt
    13501 gctgtactgc cggggtacca gaccggttga agcagcaaag tcccgactgc agctatattg
    13561 tttaccgtct cccagctgcg atgacagtac gctggcggtt atcggaccaa taccgggtat
    13621 tgtctgaaga cgttgtccta cctcatcacg actcagttcc tgctccagtg cgatttcaag
    13681 ctcggtgatc tgtccgacaa gataaatata atgggcatgt aagcgcatca gtaactgcgc
    13741 cagataggga ggaagttcgt tctctgcaag tactgttgaa agtcgtttta ccactgcgac
    13801 tcctgtcggc atactgatgc caaattccag caaaaaagca tgcatctgat tggtcgtttt
    13861 gactttatca ctgaccaatg attccctgac ccggtgaagc gcccgcatgg cctgctgagc
    13921 ttctgttcgt ggttgcacaa atcgcatgga gggacgagat gccgcttcgc atatggcttc
    13981 tgcatcgaca aaatcgttct tgttgctctt aacaaaaggg cgaacgaact gcggagaaat
    14041 caattttgct tcatgaccta aatcagcaat ccggcgagcc ataaaatgag ctccggcgca
    14101 ggcttccatc acaacggtag acgaggcaca gcctgccaga aactcaataa gtttggtacg
    14161 ggtaaacttt ttacgcagca ataccttgcc tgacttatct tggcagtgaa tgtgaaagga
    14221 atgcttgcca agatctatac caataagcgt aatgttttgc atgatggtct tctccatgaa
    14281 agaaaaacac tctactaggg tacagctcac agggtggtgg ggaccatctc attaaccatg
    14341 gaagttgaga tcctcaaaga agcggtggag tatggtcagt cgcgaaaatg gatagcgcat
    14401 gcgcccttgt tgccaacgga caaggaataa ccctggtaag ccgtattctg ggcgtgtcgc
    14461 gtgcgcaact ggcacaacgg atgaaaacat ctggtaataa gccgaataaa cgtcgtcgca
    14521 gatgtgatga ggctgcggat gcggaagtgt tagcacgtat ccttgatatc atcagcgata
    14581 tgccgacata tggctatcgt cgtgtctggg ccattctcag acgacagtcc cgtaacaaag
    14641 gagtgccgtt cgtgaatgca aagcgcgttt atcggataat gagcgaaaat aacctgttgt
    14701 tacatgacaa accttcacgg ccgcagcggg aacataaagg ccgaatatcg gtgaaggaaa
    14761 gcgaccagcg ctggtgttcc gatggctttg agttcggttg tgatgatggc gcaaaactgc
    14821 gtgtcacgtt cgcactggac tgttgcgatc gtgaagccat agactgggcc gcaagcacgg
    14881 gaggttatga caaagcaacg gtgcaggatg tcatgttagg agcaatagaa aaacgctttg
    14941 gcgataaagg agcggaacgg ccaatccagt ggctgactga taacggttcg gcttatacag
    15001 ctcatgaaac acggcagttc gccagagagt tgaatctgga gccctgcatg acggcagtca
    15061 gtagccctca gagcaatgga atggcagaaa ggttcgtgaa aacgatgaaa gaagactaca
    15121 tcgcgttcat gccgaaaccg aatgtaagaa cggcattgca taaccttgca gtggcgatcg
    15181 agcattacaa tgaaacccat ccgcatagtg cgttaggtta tctctcacca agggaatatc
    15241 gacgtcagcg ggtaacgtca acttaagata caacgactgt ctgaatatcg tgggtcaagg
    15301 acagccttct gcattttctt aatggggaac tggtaaaagc tgcaactgca tttcgtcgcg
    15361 gctttgcggc aaatacttac atcgcagaaa tactgtgcgg aaatctgcat ccatttccac
    15421 ttgctgtatg gcataacttt tcaggcggtc cggatactgc tgaagattat tatgccacat
    15481 accacccgtt atgggggcaa tatccggaag cattactgtt tgtaaactgg ctctataatc
    15541 attcctctgt gctgcatgaa cgggcagaaa tcattaaatg cgccgaaatg ctgatgcagg
    15601 aagatgattt cgaaatatgc gaaagtattt taagacagca ggagaagttg cgtgaaagaa
    15661 ttgatgagac gctttctgag aaaattgtac agaaatgcag aaatatgaat ggtgaatatg
    15721 tctggccctg gatattgccg ttttcagcgg caggcatgaa acatactggc atacagtatc
    15781 agtagatatt gcattagtgt atcctgcaca caagtaataa tttatccacc aataataaca
    15841 ctgttaatgt ccccttcccc tggttgtcag ccaggggtta tcttctgaat atttcttttg
    15901 aaaaggataa cacagtaaat tatttttatg aattatccca tggactcatt aacacccttt
    15961 cataatgctt tattgtcaaa cacgttatgg ctgacatcaa aaaaaccgga tttcctctgc
    16021 cagcgggtaa tcacctcccc ggtgttttcg gttggtctgg ttactccagt ctggtaatta
    16081 gcaagataat cgatataaac agtggaaaac tcatcgtaca taatctggtg atgaacatta
    16141 cgcttatttt cccctgaccg gaagaatcag aggctgcggt ttcagactgt ctgccggtac
    16201 attcctctcc ccgttaaaaa ccataacggg ttcattatct tcgtctgtca gcggattgaa
    16261 tggcggtata ttttcagtac gaatgccggt cagccactga aaaatacctg cgaaatgacg
    16321 ggcactgatt ttcctgctga cggactgatg agacgtgatg tcactggcgg taataatcag
    16381 gggaacgctg tagcctccct gcacatgacc atcatgatga acaggattgg cactatcgct
    16441 gaccgacagc ccatggtcag aaaagtaaag catggcgaaa tgacgggaat gccggcgaag
    16501 gataccatca agctgaccga gaaagttatc cagtttactg atgctggcga ggtaacaggc
    16561 aatctttcgg ggatactgct ccaggtaatg attcggccag gagttaagtc ggtcacacgg
    16621 attcggatga gagccaatca tgtgcaggaa aatcaccttc ggagaggatt tatccgccag
    16681 cgcacgttct gtttcctgta acaacaacat gtcatccgtt ttacgggaag caaagctgcc
    16741 tttcttgagg aaaacggtat gctccgcatc agaagcaata acagagatgc gtgtatcatg
    16801 ctcccccagt tttccctgat tggatatcca ccatgtgctg tatcctgctt ttgctgccag
    16861 cgctaccacg ttgttgccgg agtcagggtt ctgctcatag tcataaatca gtgtccggct
    16921 cagggaaggt acggtactgg ctgctgccga tgtatagccg tcaataaata aaccgggagc
    16981 cgtattcagc cacggtgtgg ttggtac
//
LOCUS       DAZGQL010000107        16932 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.107, whole genome shotgun sequence.
ACCESSION   DAZGQL010000107 DAZGQL010000000
VERSION     DAZGQL010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16932
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.107"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..174)
                     /locus_tag="ACHKIR_004130"
     CDS             complement(<1..174)
                     /locus_tag="ACHKIR_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9991796.1"
                     /translation="MARSDYDIINLSPEHELNEWLAERGYAGLADNRNRLAEVVACKL
                     QDSFCMNVSWDALN"
     gene            complement(270..893)
                     /locus_tag="ACHKIR_004131"
     CDS             complement(270..893)
                     /locus_tag="ACHKIR_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO9991797.1"
                     /translation="MRTRIDYLANKYSFTELNESPRLRWQWQDVLEECRQTEAGPEER
                     LRIALLNVDYVTSFELPFRLLLIRTPQLIAALREEWDISQKNVMFNDKRFGCVYSLKA
                     SLSGVPDTYRYHLSHRIRRVVGNESTSSPYQQVAREVKAPRERLKYALEAGLLVTALD
                     GLFWSGSQRIAADVLRLRQSGMPVVTTSVEVSDNLTGTTRKIPAYHL"
     gene            complement(982..1494)
                     /locus_tag="ACHKIR_004132"
     CDS             complement(982..1494)
                     /locus_tag="ACHKIR_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001680714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9991798.1"
                     /translation="MQITKALISEPGDIRRFVQQAVDHWPNLLAFHFTLYSAEGNING
                     QQIHAFCTSFYRQVHERITERNHTASPSSPVVLRWLREQHRGATIRCLLLLSQTSICH
                     PRASVTVDEECSQLVDLLQQTWKVISAGGQCRVEKRFQVVRGDTSGQYVALKTVVQSF
                     VLPVMTTINW"
     gene            complement(1776..2012)
                     /locus_tag="ACHKIR_004133"
     CDS             complement(1776..2012)
                     /locus_tag="ACHKIR_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO9991799.1"
                     /translation="MLTSITGHDSILLRADDPLIDMNYITSFTGMTDKWFYKLISEGH
                     FPKPIKLGRSSRWYKSEVEQWMQQRIEESRGAAA"
     gene            complement(2081..2656)
                     /locus_tag="ACHKIR_004134"
     CDS             complement(2081..2656)
                     /locus_tag="ACHKIR_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9991800.1"
                     /translation="MNAIPYFDYSLAPFWPSYQSKVIGVLERALREQSGSRIRRILLR
                     LPCEYDNTFNSRTTWFGMDFIETVSALMNAKPGRDLCWLLTRHPEKPEYHVVLCVRQE
                     YFDGPELDRLILDAWSNVLGFASPGEAAPYQKQITRDVLLDSCSPDCEDILKDLIWAF
                     SDFARDRRGVCDPEARCLAGNPGYPGSAGLF"
     gene            complement(2914..3480)
                     /locus_tag="ACHKIR_004135"
     CDS             complement(2914..3480)
                     /locus_tag="ACHKIR_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFO9991801.1"
                     /translation="MPKSYTPNWFFTALLDNHINQMLARYSCLRALRMDFFYRKDTPD
                     FLQPDHRWLELQLRMLLEQVEQFENIVGFFWVIEWTADHGFHAHAVFWIDRQRVKKIY
                     PFAERIAECWRSITHNSGSAHRCTYQPHYTYNINIPVRHNDPESIDNIRGALHYLAKE
                     EQKDGLCAYGCSEVPERPAAGRPRNLQK"
     gene            complement(5060..6664)
                     /locus_tag="ACHKIR_004136"
     CDS             complement(5060..6664)
                     /locus_tag="ACHKIR_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfjI family protein"
                     /protein_id="HFO9991802.1"
                     /translation="MNPDYVQALQTSPHFPTDVTYRLFPSDLAYLIDDLYSSTQLPLE
                     LIFNTVLATLSLSCQSLVDVVHPHTNMPEPCSLYLLAIAEPGAGKTTINRLVMTPCYE
                     FADRLIQQYEERNKDYKTELQIWNTRQKALAANLRKAVNRGDPGEQEEEALRNHERNK
                     PTRPVRPNFIYEDVSLKALAEGLNEHPEAGIISDEAVTFFRSYLKNNPGLLNKAWSGQ
                     PFDFGRADEKYHITPRLTFSLMSQPNVFTDYINKNDVLAWGSGFLSRFLFSQVGGSSR
                     VRDYRKGEFVTIPTLVEFHKKINGFLSSHSINSPGMRSERKTLKLSKKALGEWQENQI
                     KIESKALAGGEWEHIRDIVLKAGSNILRIAGLFTCYCYKDDEEITSIALWKAMHLMEW
                     YLEEASTIFYPMSERCQFEQDARELYTWIMTRIKQNNLYAIRKTDIERYGPNRLRRAE
                     KLTPVLNQLIAQNYLCIIKMRSHQALYVSVHCNNRYLLPCGAMSYEQFDIVPPLNSHN
                     AKTYHVNIPLALIPSFALPFSAYTLF"
     gene            complement(7094..7999)
                     /locus_tag="ACHKIR_004137"
     CDS             complement(7094..7999)
                     /locus_tag="ACHKIR_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO9991803.1"
                     /translation="MALEQEIANLVNDFVAGGRPSCRDQTFDERRAGYIASTALAGDT
                     ETRVNISDVIIDDILLRIFSPLNDDKLSSAIVYYHGGCFVSGGFVTHDNQFRQLAFLS
                     GCRVIAVQYRLAPEHIYPAAHDDAEHAANIVWQHAEMLKVDRNKITLMGDSAGGHLAL
                     ITALRVKNIAQWLPRKLILIYPMLDATASCDSYKLNGSDYVLTEDTFLSGYEAYFSGL
                     DLEHPEASPLFRKDFNGLPPVHIITAEYDPLRDEGEELFSRMIQQGVKCTAQRYLGVI
                     HGFFQLGGISQTARNLMQDICGIVE"
     gene            complement(8453..8905)
                     /locus_tag="ACHKIR_004138"
     CDS             complement(8453..8905)
                     /locus_tag="ACHKIR_004138"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFO9991804.1"
                     /translation="MNVIDSGIRDVKFGENVKVVMPVNLYECELGDDVFIGPFVEIQK
                     GCIIGNRSRIQSHSFICENVILGDGCFIGHGVTFANDLFKCGGPDASAESWIRIVLGN
                     TVTVGSGATILTTKICSGAVIGAGSVVTKPVLVKGVYAGNPARLLRTL"
     gene            complement(9419..10150)
                     /locus_tag="ACHKIR_004139"
     CDS             complement(9419..10150)
                     /locus_tag="ACHKIR_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HFO9991805.1"
                     /translation="MNQSIHNAYWLSRFDSLLDSAPAQHRAVSLIRVDLRFPEYMPVT
                     IMDPDPDSAVISRFFASLKAKIQAYQRKKRCANQRVHATTLRYFWCREFGKEKGRKHY
                     HVILLLNKDIWCSLGDFSEPSSLATMIQQAWCSALHPEPWQGDGLVHFSRWTPSRKPA
                     AADASPSSDDTPLHSGCSDTRTDSDKRPGGSAVLWAKRGDVEAMQKALERARYLVKNE
                     TKLHDGSGQRNYGCSRGVRCLLDGR"
     gene            10821..11351
                     /locus_tag="ACHKIR_004140"
     CDS             10821..11351
                     /locus_tag="ACHKIR_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO9991806.1"
                     /translation="MKLTEESLNLVIDILNDLICEGKMLNDVERRSLLNAAMTITAVK
                     ERSVVSVSSRKEQSKKEKKEREIDPRFPNAGVAWNEADESLLRDVLEDVPDDEIGKHL
                     FWLAEKLGRTPYSVACKVRQIKKLPAEWKDQFRKISDRIRSSGMSVSEYLQRQGLLSP
                     ESGEENNPVSHNNNVA"
     gene            13031..13834
                     /locus_tag="ACHKIR_004141"
     CDS             13031..13834
                     /locus_tag="ACHKIR_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9991807.1"
                     /translation="MSADGISDLIKPEIFLVIFPGTTTTAKRGGAGWLNNKRVVFRVW
                     LYISKASYKKMGIFQRGSAPESIPYREYHFPSYPDTHEKRKKVLRDWISFMRSRFNIN
                     QIAQFCYRMENEWAFTFDNIHVPRHFREGTDDTEWSWNYIKKKRLFSLSGLQHIQPQN
                     NTERKLLINAFFDTCLIENNPADDIDIKNMLLRKMNAAFYKRKSRARARIHSRNNQQM
                     SVSSGTDKQVNIRLKDFQYQQLLFIAQKENKSKSEIIKELITRAYRAIQ"
     gene            complement(13891..14850)
                     /locus_tag="ACHKIR_004142"
     CDS             complement(13891..14850)
                     /locus_tag="ACHKIR_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9991808.1"
                     /translation="MTGLSEQAKELHMMMGRRKPPKIQAVLNTRYWKNTEKTIKRTVG
                     MNIHTLSQWCGEQYEKMFISKVAEVYMLHHTAAILRRKGVYVDVFKIPSKIMNPPEAR
                     ANCTGCPSSQNVSPFFAYSEPNTINFSESIRINYSYTLVPAYIRKKVSSLMGDSELRY
                     VYSFPGSLYSYLLEKHPLSEVKKLMEDLPSTEDENFRRSETGSQYLKGILGVKTRVSE
                     LFIRQFSLPFRFPHTKEYVRDVIFKPVENDMSLSEELAHVLFFTLLHSDIMSRFSLRE
                     LFFRIESGRDNNKKRDLIREYVSDVTKEYSLCIPGIVSRHDKK"
     gene            14964..15848
                     /locus_tag="ACHKIR_004143"
     CDS             14964..15848
                     /locus_tag="ACHKIR_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HFO9991809.1"
                     /translation="MSFSHSSLSAQVKSYLTFLPEEIRQKILEHLHGVIHYEPVIGIM
                     GKSGTGKSSLCNAIFQSRICTTHPLNGCTRQAHRLTLQIGERRMTLVDLPGIGETPQH
                     DQEYRELYRQLLPELDLIIWILRADERAYAADIAMHQFLLNEGADPSRFLFVLSHADR
                     VFPAEEWNDTEKCPSRHQELSLATVTVRVATLFPSSFPVLPVAAPVGWNLPAFVSLMI
                     HALPPQATSAVYSHIRGENRSEQARKHAQQTFGETIGKSFDVAVARFSFPAWMLQLLR
                     KARDRIIRLLVTLWDHLF"
     gene            15967..16644
                     /locus_tag="ACHKIR_004144"
     CDS             15967..16644
                     /locus_tag="ACHKIR_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9991810.1"
                     /translation="MNTRILFPLLFTVASFSASAGNWAVKNGWCQTMTEDGQALVMLK
                     NGTIGITGLMQGCPNGVQTLLGSRISINGNLIPTSQMCNQQTGFRAVEVEIGQAPEMV
                     KKAVHSIAERDVSVLQAFGVRMEFTRGDMLKVCPKFVTSLAGFSPKQTTTINKDSVLQ
                     AARQAYAREYDEETTETADFGSYEVKGNKVEFEVFNPEDRAYDKVTVTVGADGNATSA
                     SVEFIGK"
     gene            16650..16888
                     /locus_tag="ACHKIR_004145"
                     /pseudo
     CDS             16650..16888
                     /locus_tag="ACHKIR_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278287.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
BASE COUNT         4960 a         3933 c         3527 g         4512 t
ORIGIN      
        1 gttcagagca tcccaggaaa cgttcataca gaagctgtcc tgcaatttgc aggcaaccac
       61 ctctgccagg cggttccggt tatccgccag tccggcataa cctctctctg ccagccactc
      121 attcagttca tgttccggag acagattgat aatatcgtaa tcggaccttg ctattcccgt
      181 gtccttagca catgttttca ggcacgggga taaagtataa agaatgtcgt actgctgctg
      241 ccagagcaat attccccctg atggtcgtat cagagatggt atgccggtat tttgcgggtt
      301 gttcccgtca ggttatcgct cacttccacg gacgttgtca ccaccggcat tccgctctgt
      361 cttagtctca ggacatcagc cgcaatacgc tgactgccag accagaaaag gccgtccagt
      421 gcggtcacca gtaagcccgc ctccagcgca tacttcagcc gttcacgggg cgctttcact
      481 tccctggcaa cctgctgata tggcgatgat gtgctttcat ttccaaccac gcgacgaata
      541 cggtgagaca gatgataccg gtatgtatcc ggcacaccgg aaaggctggc cttcaggctg
      601 tacacgcagc caaagcgttt atcgttgaac atcacatttt tctggctgat gtcccattct
      661 tcacgcagcg cggcaatcag ttgtggtgta cggataagca acaagcgaaa aggcagttca
      721 aaactggtga cgtaatccac attcagcaga gcaatacgca gccgttcttc tggcccggcc
      781 tctgtctgcc ggcactcctc cagaacatcc tgccactgcc agcgaagacg gggggattca
      841 ttcagctctg taaaactgta tttattcgcc agataatcaa ttcgtgtacg catactgaaa
      901 agaattctgt ataaaaattc atctgacaaa actttatcag tccataagaa tcaacggaag
      961 cgcagctggt tcacacgacc atcaccagtt gattgttgtc ataaccggta acacaaagga
     1021 ttgaacgact gtttttaacg caacatactg gccagatgta tcaccccgga cgacctgaaa
     1081 acgcttttcc acccggcatt gtccgccagc gcttatcacc ttccaggtct gttgtagtaa
     1141 atccaccagt tgtgaacatt cttcatcaac tgtgacactg gctcgcgggt gacaaatact
     1201 cgtctggctg agcagcaaca ggcatcgaat tgttgctcct ctatgttgtt cccgcaacca
     1261 gcgtaatacc accggcgagg atggactggc cgtgtgatta cgctcagtaa tacgttcgtg
     1321 cacctgtcgg taaaaggaag tacagaatgc atgaatctgt tgcccgttga tgtttccttc
     1381 tgccgaatag agcgtgaagt gaaatgccaa cagatttggc cagtggtcca ccgcctgctg
     1441 aacaaaacgc cggatgtctc ccggctctga aattaaggct ttggttattt gcacgaaatg
     1501 ttccttttca aaccctgttg aattttgtta agccggtgca gacacacgga aagcgcacat
     1561 caacaccggc acgatacgat acaaacacca gtgactggcg ggacgaaggg aatgacgaat
     1621 cagtcaggaa atgatgacaa acagttggct gaccagagga gtcagcagaa agatgagcgg
     1681 gagttgtcag cgccagcacc aggagcaggg ctgatctcca caataccagc gccgacattt
     1741 tgcacattgc cagcgtaccg gcatcacaac acgtttcatg cagctgctcc tcgtgattcc
     1801 tcaattcgct gctgcatcca ctgctccact tcacttttgt accaacggct gctgcgaccc
     1861 agttttatgg gtttaggaaa atgaccttca ctgatcagct tgtaaaacca tttatcggtc
     1921 ataccggtga aactggtgat gtagttcatg tcgatcaggg gatcgtcggc acgcagcaat
     1981 atactgtcgt ggcctgttat tgaggtcaac atagtcagga tgtcctctgt gagatttatg
     2041 gaggttgggc aaatgctggt gaatctgctg aactgagctt tcagaacagt cctgcagatc
     2101 cgggatagcc gggattaccg gcgaggcaac gggcttccgg atcgcacaca ccacgacgat
     2161 cgcgggcgaa atcactgaac gcccagatca agtctttgag gatgtcttcg cagtccggtg
     2221 aacaactgtc cagcagtaca tccctggtga tctgcttctg gtacggtgct gcttcacccg
     2281 gtgacgcgaa gcccagtacg ttgctccagg catccagaat tagccgatcc agttcagggc
     2341 cgtcgaaata ctcctgtctg acacacagca ccacgtggta ttccggcttt tcaggatgac
     2401 gggtcaggag ccagcaaagg tcgcgtccgg gtttcgcatt catcagcgca ctgacggttt
     2461 cgataaaatc cataccaaac caggtcgttc tgctgttgaa ggtattgtca tattcacacg
     2521 gcagacgaag caggatccgc cgtatccgtg agccggactg ctcacgcagc gcacgctcaa
     2581 gaacgccgat gactttgctc tgataagatg gccagaaggg tgccaggcta tagtcgaaat
     2641 aagggatagc attcataaga tatgctccat tatgcgcagc ccacaatgcg tcctgcccgt
     2701 gaataagctg acgtgttatg gtttgcggat ggggttgatg gaataagtga aaaaccgggg
     2761 caaaacggga agatcaggta actgacaagg aacaaaacac cggatgtggt tcagggtgaa
     2821 tgatctccat tgacttcggt tcacatcatt aagcagggcg gtaagttttt actaacggag
     2881 tgtttccggg ccaggaaact caaggtttaa gcttcacttc tgaagattac gtggacgccc
     2941 tgcggcagga cgctcaggaa cttcactgca gccgtaggca cacagcccgt ctttttgttc
     3001 ttctttcgcc agataatgca gggcaccgcg aatattatcg atgctttcag gatcgttgtg
     3061 gcgcacagga atgttgatgt tgtatgtata atgcggctga tatgtacagc ggtgtgccga
     3121 accgctgtta tgcgtaatag accgccagca ttcagctatc cgctccgcaa agggatatat
     3181 ttttttaacc ctctgacgat cgatccagaa aaccgcatgt gcatgaaaac catgatcagc
     3241 cgtccattca atcacccaga agaagccaac gatattttca aattgttcca cctgctccag
     3301 cagcatacgc aactgcaatt caagccagcg atgatcaggt tgtaagaaat caggcgtatc
     3361 tttcctgtag aagaaatcca tccgtaaagc tcgcaggcag gaataacgtg ccagcatttg
     3421 attgatgtga ttgtcaagta aagcggtaaa aaaccagttt ggtgtgtaag atttaggcat
     3481 gttgatgact cctgaaagtt aatgatgttt taagctctgt gaacgaatta atgctttcgc
     3541 tcataatcag atctaaaacg gtaataactt acagatggta tttatcaggc atgctgtagc
     3601 agaatggata cgacgggtta ttaacgatac gcagcattat gcagaaagaa atggattatc
     3661 tgatggtatt agctaaagag atgagataaa cgaggagcga ataaaataca gaatcaataa
     3721 acatacatca cgaaatactg caaaacacgc gataaaaacc cacgaccaat agttcaaaaa
     3781 acatattacg accaatagtt caaaaaacat attacgacca ataacatcat aaactacaaa
     3841 cttgctctca gtgagtctga aaaaacgtgt acaaggccaa catatacctg taacccaata
     3901 aacttacacg cactcattta atcggttaaa aacaactcat gttcaagata tgaacgttaa
     3961 cgggtacacg gtaccagaat aaaatatacc attgatggat aattttcatt ttgtttaaca
     4021 tacaagataa tcagaaacga acaaatataa tcaggaattc actctgttaa tttcattgtt
     4081 tatcctgcgc tgttaaacat gacggaatca tggataccgt atcaaagcgt aagcattcag
     4141 ttaatcagtg atgacagaga attgatgttc acaagaacag ttaaaacact tcagataaac
     4201 aatcatcgtt aagtcaatac agttcatgat agataagatt aaaatcaatt actgttgcag
     4261 tgaagactga taacagatgg atatcagcaa cagcgtagta gagaataact tatctgagta
     4321 tcatatgagt tacagtaatt atgattgagg gatggagtaa atacgataac aagagtaata
     4381 ctaatggaat gtgtaaagat tgagagtgag tgaagacagg aaataaataa caatagagat
     4441 tgtatgaaaa agggagtgag tataataata gtaataaacc taaattaaaa ttatccttgt
     4501 ttgatgagtc aaaatgagga attatccatt ctggatatga gtctgacaaa acaattctct
     4561 tacagaaaaa tatcaaatcg tttttcttca atcccctatt gaaagctaaa caacatctct
     4621 tatcagtaac agactgataa cctttcgtta ttcagaaaaa gatcgacatc gtgatatacg
     4681 ccagcactca tcacgacaac aatcaaacaa agttatatat aaaaactcac ataatatggt
     4741 taataattag cttcaatcca tgtgaaaaat aacatttcga ttcactttcg agatatcaaa
     4801 aacgaaaaca attcttccac cagtgcacac attcctgcat tcaaccagct ctgacaacag
     4861 gtcattatga aaacaaaatc aaatcacata catggtacat gtactgctac cgttgctacc
     4921 gtagcgattt tttcagagaa attttcgttc acaccccata attggtgaat atttgcacaa
     4981 atgcaggata tgaaaaacgc cttaccatca accttcgaat gaaggcttat gtgttttgtt
     5041 tgcttattgt aaagctgttt caaaataagg tataggctga aaaaggtaac gcaaatgacg
     5101 gaattaatgc cagagggata ttaacgtgat atgttttcgc attatgacta ttcaagggtg
     5161 gaacaatatc aaactgttcg taagacattg caccgcaagg aaggaggtaa cgattgttac
     5221 aatgaacaga aacataaagt gcctgatgcg atcgcatttt gatgatacat aaataattct
     5281 gtgcgattaa ctgattgagt acaggtgtaa gtttttctgc tcttctcaga cgattggggc
     5341 catatctctc aatgtctgtt ttcctgatag catacaaatt attctgcttg attcgggtca
     5401 taatccatgt atacagttca cgggcatcct gttcaaactg gcatcgttca gacataggat
     5461 aaaatattgt gcttgcctcc tccagatacc actccatgag atgcatcgct ttccaaagcg
     5521 ctattgatgt aatttcctca tcgtctttat agcaatagca ggtgaataaa cccgctatcc
     5581 tcagtatatt agaacctgct ttcagaacaa tatctctgat gtgttcccat tccccacctg
     5641 caagcgcttt gctttcaatt ttaatctggt tttcctgcca ctctcccaac gctttcttcg
     5701 aaagttttaa tgtcttcctt tcgcttctca ttccggggga attaatgcta tggcttgaca
     5761 gaaacccgtt aatcttttta tgaaactcca ccagggttgg tattgttacg aactcgcctt
     5821 tcctataatc acgtacccgg gaagagcctc caacctgact gaaaagaaaa cgggaaagaa
     5881 atccgcttcc ccatgccagt acatcatttt tatttatata atccgtaaaa acattcggct
     5941 gagacattaa tgaaaatgtc aggcgtggag taatatgata tttttcgtct gctctcccaa
     6001 aatcaaacgg ttgtccactc catgctttat tcaacagacc cggattattt ttcagatagc
     6061 ttctgaaaaa agttaccgct tcatcagaaa taatccctgc ctccggatgt tcgttgagtc
     6121 cttccgcaag cgctttaagc gaaacatctt catagataaa attcggtcga accggacgtg
     6181 tcggtttatt tctttcgtga ttacgcagtg cctcttcttc ctgttctccc ggatcccccc
     6241 ggttaacagc ctttcttaaa ttggcagcaa gagctttctg ccgggtattc cagatctgta
     6301 gttcagtctt ataatctttg tttctctctt cgtattgttg aatgagtcga tcggcaaatt
     6361 cgtaacaggg ggtcatcacc agtctgttta tcgttgtttt tcccgcgcct ggctctgcga
     6421 ttgccaacag ataaagtgag cagggttccg gcatgttagt gtgaggatga acaacgtcaa
     6481 ccaatgactg acaggagagt gagagcgttg ccagtacagt attaaaaatg agctccagag
     6541 gaagttgggt acttgaatac aagtcgtcaa tgagatacgc aagatcacta ggaaataatc
     6601 tgtaggttac atctgttggg aagtgggggg atgtctgcaa ggcttgcaca taatcgggat
     6661 tcattgtaat ggacctcctt cacatactgt aatcagcaca aagcttattg ttcagttcag
     6721 aaacatacca acattgtgag tatgttcgct gacgccaata ataaaatgac tgatctccat
     6781 tcaccagtcc cggcgcattt atggttctga caaaagtaaa caacatggga gccagaactc
     6841 tgcatcgcag cgacaaacta tggatgcatg aaaaacaggg aacattacgc accagagcgt
     6901 tgagagtctt cctccttgcc caatacgaaa gtcatctatc cacccgtctt caatcttcgg
     6961 gatggttgtt ttatgaacag agtaaaagtt tgaagcaagc tgagaacctc tgtttccacg
     7021 taaaggtccg ctatgaagaa aaatgggagt ttgcatgctc aactaattta cctgtaggtg
     7081 cttgttactg tagttattca acaatcccac aaatatcctg catcagatta cgggctgtct
     7141 ggctaatgcc gccaagctgg aaaaaaccgt gaatgacacc gaggtaacgc tgggcggtac
     7201 actttactcc ttgttgaatc atacgggaaa acagttcctc accttcatcg cgtaatgggt
     7261 catattccgc tgtgatgata tgaacaggtg gtaacccatt gaaatctttt ctgaataatg
     7321 gactggcttc cggatgttct aaatccagac cggaaaaata agcctcataa ccactaagaa
     7381 acgtgtcttc tgtcagcaca taatcacttc cgtttaattt gtaactatcg catgatgctg
     7441 ttgcatccag catagggtaa ataagtatta attttcgtgg taaccactga gcgatgtttt
     7501 tcacccgcag tgctgttatc agtgcaagat gtccccctgc gctatctccc attaacgtta
     7561 ttttgttgcg atcaactttc aacatttctg cgtgttgcca tacaatatta gcggcatgtt
     7621 cagcatcgtc atgtgccgca ggataaatat gttccggcgc taatcgatat tgtacagcta
     7681 tgacacgaca gccgctcagg aaagcaagct gtcgaaactg attatcatgg gttacaaaac
     7741 caccactgac aaaacagcca ccatgataat agacaattgc ggatgacaat ttatcatcgt
     7801 ttaaaggcga aaagatacgc aataaaatat catcaataat cacatcactg atgtttacgc
     7861 gggtttctgt atcgccggcc agagcagtac ttgcaatata tcccgcccga cgttcatcaa
     7921 atgtctgatc acgacaggat ggacgaccac ccgcaacaaa atcatttaca aggtttgcga
     7981 tttcctgttc aagtgccata caaatcaact ccgaccgata atatttaaac aacgctacgg
     8041 gaagtataaa tactctgacc aaaatataac aggtgaatat ccatagcttc aacaggcgta
     8101 atcaataagg gcgtacggag cttgcccgtt attgctgcag gatcgacaga ctggttttcg
     8161 tccttgcgca tgtaacagta caaccacaga gaaaagcatc aatggacact ttatgttgcg
     8221 tcagggcagc gaacgactcc ttttgttgac gacacatatc accttctgta ctggcaatta
     8281 cggcattcag agtctccctg agttttaagc tcctgctctt acggaccagc gacaagcaat
     8341 aaataactat gacaatgaat ctaataacag cataaatatg atactcatcg tgattagtat
     8401 aatcggggca gaattatatt cactgtctga tttattattg ggttaaatat aattaaagtg
     8461 ttctcaaaag tctggctgga ttaccggcat acacgccttt taccagcacg ggctttgtaa
     8521 ccacacttcc agcaccaatc actgctccac tgcaaatttt tgttgttaaa atagtcgctc
     8581 cgctacccac tgttacagtg ttacctaata cgatacgtat ccagctttct gcagaggcat
     8641 cgggaccacc acacttaaaa agatcattag caaaagttac gccatggccg atgaaacagc
     8701 catcacctaa aatgacgttc tcacagatga aactgtggga ctgaattcgg ctccggttgc
     8761 caatgatgca tcccttctgt atttccacaa agggaccaat aaaaacatca tcccccagtt
     8821 cacattcata gaggtttact ggcataacga ctttcacatt ttcaccaaat ttcacatcac
     8881 gaatgccgct gtcaataacg ttcatatccg ttgtatcctc cctcaggatg attcttatca
     8941 gaacatttca cctgtcagaa tattcccggt aatgtgatac aagatccagt ctcaccgggt
     9001 accacgtgtt attgcccgta tccttttgcc cgttacgagt ctacaactgg ttttgtattt
     9061 tgcaatccag gcctgttaca ccagagcatc ctctcacttc cccctcactg gtcagtccgg
     9121 gattcaacat catcctccag cactaaccct ataccagtcc tgaaccttcc ctcgccagca
     9181 gcctactgtt caccacaatc ctcttccgtg aatggcagta agtgagctgc accaaaataa
     9241 atcggtactt ccgatgacag acagtggaag tgccaggaga aatatgaaca tgagaatgga
     9301 agcagataaa aataccgttg catgacttca ggacgggtta tcagaatacg agctaagggc
     9361 gtcagccaac gcccttaccg tggtatacac tcaggcaccg gataccattt taccgggttc
     9421 acctgccatc cagtagacac ctcaccccac gactgcaacc ataattacgt tgacctgaac
     9481 cgtcatgcag cttcgtttca tttttcacga gataacgggc tctctccagg gctttctgca
     9541 ttgcttccac atcaccacgc ttggcccaga gcacagcgga tccacccggc cttttatcag
     9601 aatccgtccg ggtatcagaa catccactat gcaaaggcgt gtcatcggaa gaaggggaag
     9661 catcagccgc tgctggttta cgggaaggcg tccacctgga aaaatggacc aggccatcgc
     9721 cttgccaggg ctcaggatgc agagcgctgc accatgcctg ttggatcatc gtcgccagcg
     9781 aagacggttc gctgaaatcc ccgagcgagc accagatatc tttgttgagc agtaatatca
     9841 cgtgataatg tttcctgcct ttttccttgc caaactcccg acaccagaaa taacgcaggg
     9901 tggttgcatg cacacgctga ttggcacatc tttttttccg ctggtaagcc tgaattttgg
     9961 ctttcaggga tgcaaaaaaa cgagaaatca ccgctgaatc cggatcggga tccatgatgg
    10021 tgacaggcat atactcaggg aaacgtaaat ccacccggat taacgagacg gcacggtgct
    10081 gcgccggggc actgtcgaga aggctgtcaa aacgggataa ccagtaagca ttgtgtattg
    10141 attgattcat gatgagagat ctccgttgat ttggattgac ttcacatcat tcatcaacgc
    10201 ggtgaaactt tacggcatcc cgccaatgag caacgccaat agctcctgat taaaacggaa
    10261 tgaatcgtcc cgtaacgaaa agataatcct gcacacgaca gcaaaacgta aaacaacagc
    10321 atgaatgagc taaatgttta aagaaaaaat gtcttttcac attatttttc agcaagtaat
    10381 cataccagca tccctaacat acgaaatcac atagtgatcg atcgctgatt cgcttaacaa
    10441 ctgatcgaat acaccggaat ttacactaac atcctgatgg gtaacggaag actaaaaaac
    10501 ctgtgttcta ttgattgcac ggaatgcctg tgcactggcc tgaccagtaa gcacaattct
    10561 acgagtagta agatatctac gccagaacca tgcggcagta gccaccatat gttgccaggg
    10621 tgaaaaataa gctcagagct ataggggagt aattcccgtt aatgtcatcg ctgtacctca
    10681 atcagttcac aaactggctg aaacgttaag ctgaagcctt tattcttcag atgtattttc
    10741 cggtttacct gtaaattatc ttctgatatt tatcacatta gtagttgtgc tgattatcta
    10801 catacttcaa ggtctgaaat atgaagctaa cagaagaaag tctgaatcta gtcattgaca
    10861 tcctgaatga tttgatctgt gaaggaaaaa tgctgaacga tgtggagcgc agatctctgc
    10921 tcaatgcagc tatgacaatt acagcggtaa aagaacgttc tgtcgtatct gtatcatccc
    10981 gaaaagagca aagtaagaag gagaagaagg aaagagagat cgatcctcgt ttcccaaatg
    11041 ctggtgtggc atggaatgag gcagacgaga gtttattgcg agatgttctg gaggatgtac
    11101 cggacgatga aattggtaaa caccttttct ggctggcaga gaaactaggg cgaaccccgt
    11161 acagtgtagc ctgtaaagtc agacaaatta agaaattacc tgcagaatgg aaggatcaat
    11221 ttcgtaaaat ctctgacagg atccgtagtt ctggaatgtc agtcagtgaa tatctacaac
    11281 gccagggact gttaagccca gagtcagggg aagaaaacaa tcctgtcagt cataacaaca
    11341 atgtagctta atcctgaaga agatgaacag tttatactct ttaaactgtt cccttaattt
    11401 tagaaaaaca gtttgtgtaa gtgcatgctc caacattctg aaggagttat ttcttacaat
    11461 acatcataat cattggcaga tcctcatctg aatcaaaaca ccacaaccaa tcatcacact
    11521 ttttctgaaa agagccttat gatgaagata atcaccttac gctgagaaaa aacaccaggg
    11581 ccagttaatc accttatcaa tctcgccgtt acaaaatcat ccataagcca tttaacagtg
    11641 ttatcgcaaa ctcacagtaa atcgcatcag gttacctcaa tcggatttga gttaattcac
    11701 tgggctttac gtaaagatac ttttctcagg ttaacgacta aaacacgaaa actcagaaaa
    11761 ggttaaacac aggttcagta aaatcgaata tcatcttgat tgcttaaaca ggacagaggg
    11821 ttttatagtg aggttaactg attttagcga tcgtaaaaaa aggactaaca aatttactaa
    11881 actggatgtg tatcagacaa agcaatgtgt atagcaatgc ttaatgaaaa tacttgtatg
    11941 attgtctatg aaagtacgtg tatatagtat cataccgaca ttctcaactc ttccaaacat
    12001 ccatgcagac tgaatgttgg tttcagtttt tcacggccag catggggatt tttgttctga
    12061 aaacggagca gatttactct ggaattattt tccctgtcgg gatatttttc acctgaaaac
    12121 tttctacaac acaacggctg tataacatca ttctggcata ttcattcagg tgagtcggac
    12181 aagcgataat cctgttcttt accgaacgaa caggcagcga taaaaaactg attcagacga
    12241 catttctgtc tgacaaacat tcacatgcag aagaccggga catttattct tcccgaaagg
    12301 aagtgatacc ttttcttatc atggtagctg cattacctga tatctttcag gcgaatcccc
    12361 tccctgtaaa tctgatcgtc agataaaaga gatgaacatc aggcagattt ccgtcaataa
    12421 cgataagaga tgaccatcgt aaacatatct tccagtaacg aactggcacc tgcatattac
    12481 cgtttattct catacatcat tgaaatattg tcagttataa aggacacaca tcaggaaatt
    12541 atcaccatgt gacaacgtca cagatggtgc tgatatataa aaattaacaa aatcgcgcca
    12601 aaaaaccgcg ccgatattgc gcatctgtaa tccatcattt cctttttgaa taaccttccg
    12661 tggttacccg acatcctgat aatgacgccc atcgtacacg cttttctttg ctcagacatc
    12721 gcatactttt atcctgctca ataccgtctt cctgccagtc ttcccactct ctgacactca
    12781 cagaacagca acgtcctccg gacatactag cagggcagaa cacaatccac aacattaagt
    12841 atatgtaaca ataatatacc attaagacca cttctatgac cttatatgtt acaaccttag
    12901 agcatgtatt taatatccca taatacagac tcgtcattgt tcttctgaga cgacatgggc
    12961 atgtcacaaa actaattgac tgacatatca tgaaaatgtc agtatttaat aatttaatgg
    13021 aggtacaaac atgagcgcag atggcatttc cgatttaata aaaccagaaa tatttcttgt
    13081 catttttcct ggtacaacaa ccacagcaaa acggggtggc gccgggtggt tgaataataa
    13141 aagagtcgtg ttccgggtat ggctttatat cagtaaagcc agttataaga agatggggat
    13201 ttttcagcgc ggttctgcgc ccgaatcaat accctatcgt gaataccatt ttccctcata
    13261 ccctgacacc catgaaaaaa ggaaaaaggt attacgtgac tggatatcct tcatgcgctc
    13321 ccgttttaat ataaatcaga tagctcagtt ctgttatcgt atggaaaatg aatgggcatt
    13381 cacctttgat aatatccacg ttccacgaca ctttcgggag ggaactgatg acacagaatg
    13441 gtcatggaat tacattaaga aaaaacgatt attttccctg agcggcctgc aacacattca
    13501 gcctcagaac aatactgaac gaaagcttct cattaatgct ttttttgata cctgccttat
    13561 tgaaaacaac cctgctgacg atattgacat aaagaacatg ttactcagaa agatgaatgc
    13621 agcgttttat aaaagaaaga gccgggcgcg tgcgcgaatt cactcccgga ataaccaaca
    13681 gatgtcagtc agttcaggaa cagataaaca ggtaaatatt cgtcttaagg attttcagta
    13741 tcagcaactt ctttttatcg cgcagaaaga aaacaaaagt aagtcagaaa tcataaagga
    13801 actcattacc agggcatacc gggcgataca ataaccacca cgttcaccat ttcatcccga
    13861 ccatacatca tatgcacggg atgatactga ctatttttta tcgtgccgac tgactatgcc
    13921 aggtatacat aacgaatatt cttttgttac atcgcttaca tattcgcgta taagatccct
    13981 tttcttatta ttatcacgtc cactttctat tctgaagaat aattctctta aactaaacct
    14041 ggacattata tccgaatgca acaatgtaaa aaataacaca tgggccagtt cttcacttaa
    14101 cgacatatca ttttctacag gcttaaatat aacgtctctt acatattctt tagtgtgggg
    14161 aaatctaaac ggcaaagaaa actgccttat aaaaagttca gatactcttg tttttacacc
    14221 gagaatcccc ttcagatact ggctacctgt ttcactcctc ctgaagtttt catcttccgt
    14281 tgaaggcaaa tcctccatca gttttttaac ttctgacaat ggatgtttct caagaagata
    14341 agaatataaa ctaccgggaa atgaatacac ataacgaagt tctgaatccc ccatcagaga
    14401 tgagactttt ttccttatat aggctggaac tagagtatat gaataattta ttcttatact
    14461 ttcagaaaaa tttatggtgt taggctctga ataagcaaaa aaaggagaga cattctgaga
    14521 agaagggcaa ccagtacaat tggctctcgc ttcaggcggg ttcattattt ttgagggaat
    14581 cttaaaaaca tccacataaa ctcctttacg cctcaatatt gctgccgtat ggtgaagcat
    14641 atagacttct gccacttttg aaataaacat cttctcatat tgttctccac accactgact
    14701 gagcgtatga atattcatac ctacagttct ttttatcgtt ttttctgtgt ttttccagta
    14761 tcgggtgtta agaacagcct gtatcttcgg cggttttctc cgccccatca tcatatgaag
    14821 ttcttttgcc tgctcagata atcctgtcat ttattacctc cgaatatatt ccattaaaaa
    14881 tggaaataat tatacgccca tattacctga atgataaacc ggattactgt gatccggttc
    14941 agtgaaatca acaggatacc ggtatgtcat tcagccattc ttccctctct gcacaagtga
    15001 aatcatatct gacgtttctt cctgaagaaa ttcgccagaa aatccttgaa catctccacg
    15061 gtgttattca ttacgagccc gtgattggca ttatgggtaa atccggcacc ggcaaaagca
    15121 gtctgtgtaa tgccattttt cagtcccgta tctgcaccac gcatcccctg aacggctgca
    15181 cccgccaggc tcatcgcctc accctgcaga ttggtgaacg cagaatgacg ctggtcgatc
    15241 tgcccggcat tggtgaaaca ccgcagcatg atcaggaata ccgggagctt taccgtcagt
    15301 tactgccgga actggatctg attatctgga tcctgcgggc tgatgaacgt gcgtatgctg
    15361 ccgatattgc catgcatcag tttttactga atgaaggtgc agatccctcg cgttttctgt
    15421 ttgtactcag tcatgccgat cgcgtgtttc ctgctgaaga atggaacgac acagaaaaat
    15481 gcccgtcccg tcaccaggaa ctctcactgg caactgttac agtccgggtg gcaaccctgt
    15541 tcccgtcatc atttccggta ctccccgttg ctgcgcctgt cggctggaat cttccggcgt
    15601 ttgtgtcact aatgatccac gccctgccac cgcaggcaac cagtgcggtt tattcacata
    15661 tccgggggga aaaccgctct gaacaggccc ggaaacacgc acaacagact tttggtgaaa
    15721 ccatcgggaa aagttttgac gtcgccgttg cccgtttcag ttttccggcc tggatgttac
    15781 agcttctgcg taaagcccgg gaccgcatta tccgcctgct ggtcacactg tgggatcacc
    15841 tgttctgagc gccccggagt cccccgtggc aaccgtatac ctttacacga caacggatta
    15901 cggtaaccac tgccgtatac cgtctgtcat tatcaataac acactcttta atttcagagg
    15961 tcagtcatga ataccagaat tctttttccc ctgctgttca ctgtggcatc cttctccgcc
    16021 tccgccggta actgggctgt caaaaacggc tggtgccaga ccatgacgga agatggtcag
    16081 gcgctggtaa tgctgaaaaa tggcacgatt ggtattaccg gcctgatgca gggatgcccg
    16141 aatggcgtac agacgctcct gggcagccgt atcagtatta acggtaacct gatccccaca
    16201 tcacaaatgt gtaatcagca gacgggattc agggctgttg aggtggaaat cggacaggcg
    16261 ccggaaatgg tcaaaaaagc cgttcactcc atagcagagc gtgatgtgtc cgttttacag
    16321 gcatttggtg tacgaatgga attcacccgc ggtgatatgc tgaaggtctg tccgaaattt
    16381 gtcacatcac ttgccggttt ttccccgaaa cagacgacca ctattaataa agactccgtc
    16441 ctgcaggctg cccggcaggc atacgcccgg gaatatgacg aggaaacaac agaaaccgct
    16501 gattttggct cttacgaagt aaaaggcaat aaggttgagt ttgaagtatt caatcctgaa
    16561 gaccgtgcgt acgacaaagt gaccgtcacg gttggtgctg acggtaatgc caccagtgcc
    16621 agcgttgaat ttatcggaaa atagccggta tgtcggactg tcaccctgtt ttattgcccg
    16681 aaggcccttt ctcacgcaaa caggcaatgg ctgccacaac agcttacctt accgcaatgt
    16741 gcttattgaa gatgaccatg gaacgcattt cctgctggtt atccgcaatg ccgaagggca
    16801 gctacgctgg cggtgctgga attttgagtc tgacgccgga aaacagctta atgtgtggct
    16861 cgccagtgaa gggcttctca gacaataaaa gtcttcattt catccatcag gccgcgtctt
    16921 ctccgggaga cg
//
LOCUS       DAZGQL010000108        16838 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.108, whole genome shotgun sequence.
ACCESSION   DAZGQL010000108 DAZGQL010000000
VERSION     DAZGQL010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16838
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.108"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(188..907)
                     /gene="dcuR"
                     /locus_tag="ACHKIR_004146"
     CDS             complement(188..907)
                     /gene="dcuR"
                     /locus_tag="ACHKIR_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HFO9991811.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(904..2535)
                     /gene="dcuS"
                     /locus_tag="ACHKIR_004147"
     CDS             complement(904..2535)
                     /gene="dcuS"
                     /locus_tag="ACHKIR_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFO9991812.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            2715..2945
                     /gene="yjdI"
                     /locus_tag="ACHKIR_004148"
     CDS             2715..2945
                     /gene="yjdI"
                     /locus_tag="ACHKIR_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HFO9991813.1"
                     /translation="MDQALLDRGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVATVVKVIDTCPSGALKYRHK"
     gene            2957..3229
                     /gene="yjdJ"
                     /locus_tag="ACHKIR_004149"
     CDS             2957..3229
                     /gene="yjdJ"
                     /locus_tag="ACHKIR_004149"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO9991814.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            3456..3752
                     /gene="ghoS"
                     /locus_tag="ACHKIR_004150"
     CDS             3456..3752
                     /gene="ghoS"
                     /locus_tag="ACHKIR_004150"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFO9991815.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMSFSSIVADEY
                     GIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLNIMTVDDYFRQFYK"
     gene            3780..3953
                     /gene="ghoT"
                     /locus_tag="ACHKIR_004151"
     CDS             3780..3953
                     /gene="ghoT"
                     /locus_tag="ACHKIR_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HFO9991816.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
     gene            complement(4072..5589)
                     /gene="lysS"
                     /locus_tag="ACHKIR_004152"
     CDS             complement(4072..5589)
                     /gene="lysS"
                     /locus_tag="ACHKIR_004152"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFO9991817.1"
                     /translation="MSERETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKDNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIVTEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPLK"
     gene            complement(5826..7283)
                     /gene="dtpC"
                     /locus_tag="ACHKIR_004153"
     CDS             complement(5826..7283)
                     /gene="dtpC"
                     /locus_tag="ACHKIR_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HFO9991818.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMIKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESR
                     GNSALRVWLKFAFGLLLMACGFMLLAFDARHAAAGGQASMGVMISGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMAIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMIQESND"
     gene            complement(7342..9489)
                     /gene="cadA"
                     /locus_tag="ACHKIR_004154"
     CDS             complement(7342..9489)
                     /gene="cadA"
                     /locus_tag="ACHKIR_004154"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HFO9991819.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAADDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHATIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYTNFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVKGDVNEETFNEA
                     YMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEIKRLRTESDGWF
                     FDVWQPDHIDTTECWPLRSDSSWHGFKNIDNEHMYLDPIKVTLLTPGMEKDGTMSDFG
                     IPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTDFKRAFDLNLRV
                     KNMLPSLYREDPEFYEDMRIQELAQNIHKLIVHHNLPDLMYRAFEVLPTMVMTPYAAF
                     QKELHGMTEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEFLQMLCEI
                     GAHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
     gene            complement(9569..10903)
                     /gene="cadB"
                     /locus_tag="ACHKIR_004155"
     CDS             complement(9569..10903)
                     /gene="cadB"
                     /locus_tag="ACHKIR_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043872 - lysine:cadaverine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HFO9991820.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTAGTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAGSGAPFAISASTILGNWAAPMVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKASDLFGELTGIAVLLTMLPYFYSCVDLIRFEGVNIRNFVSLICSVL
                     GCVFCFIALMGASSFELAGTFIVSLIILMFYARKMHERQSHSMDNHTASNAH"
     gene            complement(11269..12807)
                     /gene="cadC"
                     /locus_tag="ACHKIR_004156"
     CDS             complement(11269..12807)
                     /gene="cadC"
                     /locus_tag="ACHKIR_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
                     /protein_id="HFO9991821.1"
                     /translation="MQQPVVRVGEWLVTPSINQISRNGRQLTLEPRLIDLLVFFAQHS
                     GEVLSRDELIDNVWKRSIVTNHVVTQSISELRKSLKDNDEDSPVYIATVPKRGYKLMV
                     PVIWYSEEEGEEIMLSSPPPIPEAVPATDSPSHSLNIQNTATPPEQSPVKSKRFTTFW
                     VWFFFLLSLGICVALVAFSSLDTRLPMSKSRILLNPRDIDINMVNKSCNSWSSPYQLS
                     YAIGVGDLVATSLNTFSTFMVHDKINYNIDEPSSSGKTLSIAFVNQRQYRAQQCFMSV
                     KLVDNADGSTMLDKRYVITNGNQLAIQNDLLESLSKALNQPWPQRMQEMLQQILPHRG
                     ALLTNFYQAHDYLLHGDDKSLDRASELLGEIVQSSPEFTYARAEKALVDIVRHSQHPL
                     DEKQLAALNTEIDNIVTLPELNNLSIIYQIKAVSALVKGKTDESYQAINTGIDLEMSW
                     LNYVLLGKVYEMKGMNREAADAYLTAFNLRPGANTLYWIENGIFQTSVPYVVPYLDKF
                     LASE"
     gene            complement(13067..13178)
                     /locus_tag="ACHKIR_004157"
                     /pseudo
     CDS             complement(13067..13178)
                     /locus_tag="ACHKIR_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218818.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(13618..14460)
                     /locus_tag="ACHKIR_004158"
     CDS             complement(13618..14460)
                     /locus_tag="ACHKIR_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFO9991822.1"
                     /translation="MSDITVSRPEVVNWHTDVICSTSILHILAVRKSTLLQIDTLIRQ
                     LAEISAMTESIGGKTALDWAMKQNFHCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDAQARDTWYRSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVINVFRGLS
                     WNYKTNCPCKFGSKIIVNNLVRWDRWGFHLITGQQADRLADLEIMLHLFSGKPIPDNR
                     ENITIHLDDHVQSVQGKEDYEDEMFSIRYFKKGSAHITFRKPELVDRLNDIIAKHYPG
                     ALAI"
     gene            complement(14545..14742)
                     /locus_tag="ACHKIR_004159"
     CDS             complement(14545..14742)
                     /locus_tag="ACHKIR_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFO9991823.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAREDIRTLRQLQLLHQNR"
     gene            complement(14754..14993)
                     /locus_tag="ACHKIR_004160"
                     /pseudo
     CDS             complement(14754..14993)
                     /locus_tag="ACHKIR_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
     gene            15060..16222
                     /locus_tag="ACHKIR_004161"
     CDS             join(15060..15321,15321..16222)
                     /locus_tag="ACHKIR_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3 family transposase"
                     /protein_id="HFO9991824.1"
                     /translation="MTKTVSTSKKPRKQHSPEFRSEALKLAERIGVTAAARELSLYES
                     QLYNWRSKQQNQQTSSERELEMSTEIARLKRQLAERDEELAILPKGRDILREAPEMKY
                     VFIEKHQAEFSIKAMCRVLRVARSGWYTWCQRRTRISTRQQFRQHCDSVVLAAFTRSK
                     QRYGAPRLTDELRAQGYPFNVKTVAASLRRQGLRAKASRKFSPVSYRAHGLPVSENLL
                     EQDFYASGPNQKWAGDITYLRTDEGWLYLAVVIDLWSRAVIGWSMSPRMTAQLACDAL
                     QMALWRRKRPRNVIVHTDRGGQYCSADYQAQLKRHNLRGSMSAKGCCYDNACVESFFH
                     SLKVECIHGEHFISREIMRATVFNYIECDYNRWRRHSWCGGLSPEQFENKNLA"
     gene            complement(16472..>16838)
                     /gene="mchS3"
                     /locus_tag="ACHKIR_004162"
     CDS             complement(16472..>16838)
                     /gene="mchS3"
                     /locus_tag="ACHKIR_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MchS3 family protein"
                     /protein_id="HFO9991825.1"
                     /translation="PPPLQQSLEKTTYFSIGMNGFIGYQSEGEKLYTHILTLDNPEEI
                     FKNIIKNRKSTKESKIYAACGLYYLNVENIESLFNENDKQEYVSVLRGDILTKIKLND
                     ILNSVIINGCNTKLISEHK"
BASE COUNT         4574 a         3885 c         3912 g         4467 t
ORIGIN      
        1 taattaactt ttgttagcgt tttgaaatta aaaacaccgt tcatctgaag agatattaat
       61 ttttaacgat gatggaggga taattatatt tgatctggca caagttttac tgatgaagaa
      121 tgtaacttgt gccaggaata ttttgcatta cagtaataat tattacttac agataaccag
      181 cgatccgtta ttggcaatat tgtttcagca gtgagtagtg ttctgcctga atacggtaac
      241 ggtaaaccgg acgccctgtg acgccataat ggatactggt gaacaagatg tggcagttga
      301 ccagccagat gaggtattta cggcaggaaa cacgcgagat gttaacctcg ttggccagct
      361 cgtcggttga aaactcatag tcctgatgcg cgtcaatcca ctggcacagc gtgcgtaacg
      421 tctgcggcgt caagcctttt ggcaagcgac gaggatcctg ttcgttggag ctgctgccgt
      481 ggattagctg atcaagctcg gcctggtcat aatactgatg tttttccagc gccattttct
      541 tttgccgcca gccggtgagc gcctcttcaa agcgggaagc ctggaagggt ttgatcaggt
      601 aatccacgac accgtaatgc agcgaatctt taatggttgc cgcatcggct gcggaggaga
      661 tgacaatcac atcacttttg caacgcgcgt tatgcaggac aggcagtaaa tcgagcccgt
      721 tctctttttg catatagata tcgagcaata tcaggtcgat aggcgtatcg ctattgaaga
      781 taatctcttt ggctttctcc agcgtcgaag ccgttccaca gcattgaaag cctgggattt
      841 gtgccacgta tcggcgattc agctccgcga ccattgcgtc gtcatcgata attaatacat
      901 tgatcatctg ttcgacctct ccccgtccca gggtatctgg acaaaaaatt gtgtgaaaat
      961 cccgggttcc gattccacgg cgatgctgcc gccgagattt tctacctgtt gtttgacaag
     1021 tgctaaaccg acgcctcgct cgcttccttt tgtcgagaca cctttgtcaa aaatgtgatc
     1081 gattttgtcg ggtgcgatcc ccggtccatc atcgttaact tcacagtgca gccagccgtg
     1141 acggtagtgc aatgttacgc taatttcgcc accgggttcc ggtcctaatg cctccagcgc
     1201 gttttctatc agatttccca gcgtggtaat cagcgtcgcg acctggtctt cactgccgct
     1261 gtctggcaac tggctttcac tgtttaaaat cagcgtatgg cctaaatcgg tcgcgcggtt
     1321 aatcttactg attaaaaaac cagcgataac cggagatttg atcttaccca gcagagagcc
     1381 aatctcttct tgatagttat tggctgtttt gagaatgtaa tcttccaact gcttataact
     1441 cttcagatgc aataatccga gaatcacgtg caatttattc ataaattcgt gggatcgttc
     1501 acgaagtgcg tcagcatagt tgaccagacc atcgagtcgc tgcatcagtt tacgtacttc
     1561 agttttgtcc ctgaaggttg aaatggcacc gatgataacg ccattactgc gcaccggaac
     1621 ggtgttgatc agtaatagcc ggtctttaat cgtaatctct tcgtcgcgcc gcggggtacc
     1681 gtcgcgtaac acttccgaga catctaccac ctgtgaccac gagtggctta gcgtcgacag
     1741 cttctcatcg tcctgcgact tacggtaatt cagcaattct tgtgcggcat cgttgatcag
     1801 cgtgacttca ccgcgatcgt ccacggcaac gacgccttct ttgatggact gcaacatagc
     1861 ctggcgttgc tcaaacagcg tggagatttc gtagggttcc aggccgaaaa ggattttttt
     1921 cagtacctta accagaatgc aggtgccaat cagcccgacc agcatgccaa ataataccga
     1981 ccagatgatg ctccagcgac tgtcattgat ctgttgggtc acgcgactta actcaaggcc
     2041 gatcgccacc acgccaattt gtttatgatt ttcatcgtag atgggggtaa atacgcgtaa
     2101 agcctgcgcc agaaaaccgc gattgatagc gacattttct tcgccattca gcgctttaag
     2161 gatgtcatca cctttaaatg gctgaccaat acgctgggct tcaggatgcg agtagcgaag
     2221 actttgcata tcggtaacga caataaacag cagatcgttg cgtttgcgca cggcttccgc
     2281 gatggcctgg attccactct cctgtggttt tttctgcaag ccctgacgga tttccggcga
     2341 gtcggcgagg gtacgcgcta ctgccagtgc cttgttagct agcccgtctc gcgtcatatc
     2401 actgatttgc gagaagtaaa tcagatgcac caccaatagc accgagaaca gtaccgcgct
     2461 gaccattaag atcactgtgg tactcaattt catcggacgt ttgcgtaaca tgcggtaggg
     2521 caatgaatgt ctcatcagct tccttgtgtg acaaatttct taagcattat ctctgatgag
     2581 gcgggtaatt caaagggagt aagaatgatt ggcatatagg ggaagagact ctggcaacgg
     2641 aaactgccag tgctgtatga agattccagg gctatgctta tagtgaaaac catactgatg
     2701 agagagggaa ggtcatggat caggcgctac tggacagggg ttatcgctgt tataccggcg
     2761 aaaagatcga tgtctatttc aacactgcga tatgtcagca ttctggcaat tgcgtacgtg
     2821 gcaacggcaa gttatttaat ctcaaacgaa agccgtggat catgccggat gaagtcgacg
     2881 tcgccactgt ggttaaagtg attgatacgt gcccgagcgg cgcgctgaaa taccgtcata
     2941 aataagcgag ggtaaaatgg aaatacgcga aggccacaat aaattttaca ttaatgacga
     3001 acagggcaag caaatcgctg aaattgtctt tgtgccgacc ggagagaatt tagcgattat
     3061 cgaacatacc gatgtcgatg aaagcctgaa agggcaaggg attggtaaac agctggttgc
     3121 gaaagtcgtg gaaaaaatgc gtcgggaaaa acgaaaaatt atcccactat gcccatttgc
     3181 gaaacacgaa tttgataaaa cgcgggagta tgatgatatt cgcagttgat gggagagtac
     3241 agagtcacga tatttttcat gctctccgcg gtgtgatgca ggagagcatc tgaagggtag
     3301 ggggatgcac aaagaatggg cagagagcgc gtttttttgt cccaagtcat cccctttact
     3361 gagcaaaaaa aagaatatct cctatatgag aatcatcaat cggggttaat aagttttgcg
     3421 tccccagagc gtttaatatt gataggagtc atattatgga aggtaaaaac aagttcaata
     3481 cttatgttgt ttcttttgat tatccatcat cttattcctc agtgttctta aggttaagat
     3541 cattgatgta tgatatgagt ttctcctcta tcgtggctga tgaatatggg ataccacgac
     3601 aactgaatga aaactccttc gcaataacga catcgttagc cgcaagtgaa atcgaagatt
     3661 taatcaggct taaatgctta gacttaccgg atattgactt tgacctcaac attatgacag
     3721 ttgatgacta tttccgtcag ttttacaagt agccaggaag gataagaaga acataaaaaa
     3781 tggcactatt ctctaaaata ttaatttttt atgtgattgg tgtgaacata tcctttgtca
     3841 ttatctggtt tatctcacat gagaaaacac atattcgttt acttagtgca tttctggtcg
     3901 gaataacctg gccaatgagt ctgcctgtgg cattactttt ttctctcttt taggagctgt
     3961 agttgagtgg acccgctggt tatcttgaaa cgaagcgtct ctcctcaaaa ctggatttgc
     4021 ggaatgacga acgaagcagt cgcctgactg cttcgttcat taaagtgaaa tttatttcag
     4081 tgggcgcatc gccgggaaga gaataacgtc gcgaatagta tgactgtttg taaacagcat
     4141 aatcattcgg tcgataccaa tacccagacc agcggttggc ggcagaccat attccagcgc
     4201 agttacgtaa tcttcgtcat agaacatggc ttcgtcgtca cctgcagctt tagcgttaac
     4261 ctgttcctgg aaacgttcag cctgatcttc tgcgtcgttt aattcgctaa aaccattacc
     4321 aatttcacga ccaccgatga agaattcaaa acggtcggtg atttccgggt taacatcatt
     4381 acggcgcgcc agcggtgaca cttctgccgg atattccgtg ataaaggttg gctgaatcag
     4441 atgtgcttct gccacttcat caaagatctc cgtgacaatg cgtcccaacc cccagctttt
     4501 ctctaccgta attccgatag attcagctaa tgctttagca gcatcaaaat tatccagatc
     4561 cgccatatcg gtttctggac gatatttttt gattgcttcg cgcatggtga gtttttcaaa
     4621 cggtttgccg aaatcaaaca catgctcgcc ataagtgact ttagtggtac ccagtacctc
     4681 ttgtgccaga gtgcggaaca gtgattccgt cagttcaatc aaatcgtggt aatccgcata
     4741 cgccatgtag agttccatca tcgtgaactc aggattatga cgaacagaaa taccttcatt
     4801 acggaagtta cggttgattt cgaatacccg ttcaaaacca cctacaacca gacgtttcag
     4861 atatagctcc ggcgcgatac gcaggtacat atctaaatcc agagcgttat gatgggtaat
     4921 aaacgggcga gcagatgctc cacctggaat tacctgcatc atcggggttt ctacttccat
     4981 aaagccgcga gcgaccatga actgacggat agcggccaga atttttgaac ggacaacaaa
     5041 cgtttgacgg gatttatcgt tagcgatgag gtccagataa cgttgacgat aacggacttc
     5101 ctgatcctgc agaccatgga atttatctgg taaaggacgt agtgctttag tcagcaggcg
     5161 cagctcagta cagtgaatgg aaagctcacc cgtttgcgtc ttaaacagcg taccgcgggc
     5221 accgataatg tcacccagat cccatttttt aaactgatcg ttataaacac cttctggcag
     5281 gctatctctt gcaacgtaca gttgaatacg gccaccgaca tcctgcaacg ttacaaagga
     5341 ggctttcccc ataatacgac gggtcatcat tcggccagca accgagactt caatgtttaa
     5401 ggattccagt tcctggttat ccttcgcatc aaactcttcg tgcaactggt cagaggtatg
     5461 gtcgcggcga aaatcattgg gaaacgccac accttgctga cgtagtgccg ccagtttttc
     5521 gcggcgattt ctcagttcat cgttaaaatc aatagcctca ttggctcccc gtgtttctcg
     5581 ttcagacatt ttggttcctc taaatccagc tttcaattta gcgtagataa agagacagat
     5641 cggtacactg tattatctgc ctcgactatt aataactcag tttatcttat cgaaactaac
     5701 gcttacctca agttgatatc actaataaat aaccataaaa atggtttaaa tgacttatta
     5761 atctcgattt gctagtgtga taaactgaaa gttaatttat tcataaccaa ttgtttttac
     5821 gacagttaat cgttgctctc ctgtatcata ttcgtcggcg tgctaaacag aaaacgggta
     5881 gcaaaggcca ataccacgat gatcgcgaca caagccaacg tccactctcc catctgagaa
     5941 aagaatcgct ggtaagcggc aattgccata ccgctaattt gcgactctgt cgtctgctgt
     6001 gccacgacgc ctgccagcca gttggcgacc gcgcctgttg ccagcatata aataccggtt
     6061 aatacgccag acattttcag acgcgtgatt tgcgcaatcg ccaccgggtc aataaagagt
     6121 tcagcaaagc ccattagcgc cagcccggat atcatcacgc ccattgacgc ttgaccgcca
     6181 gccgctgcat gtcgggcatc aaatgccagc aacataaagc cacaagccat cagcagtagg
     6241 ccaaaggcaa acttcagcca gacgcgcaat gctgaattgc cgcggctttc tggcgacgcc
     6301 agccaggcca gaacaacccc agcgagcatc accgcaatag cattcaccga ctggaatagt
     6361 gctgtaggta cttcaatatt gaatgcctga cgattcacaa agcgatcgat aaacaagctg
     6421 atggtactgc cgccctgttg tgccagtacc cagaacaacg tcccgacaaa catcaacaat
     6481 acaatttgcc aaagagcacg gcggtgttcg gggaatttga tcatcatgcg ggcaatgatt
     6541 tgtgctgcaa tgaggcaaac gatcgccagc aaatatccgg accagtcgtt ctccagcagc
     6601 agggtaaaaa atactggggc taaacagagc atcaccacta accagctcca tactggtaag
     6661 gcaaatttga cgctagtgag cgctttttta tccatactac gcgtggactg gaaatgacga
     6721 tgaccactta agaaaatcaa caaaccgata aacatgccgc ctcccgcaag ggcaaagcca
     6781 acatgccatc cataccactg agcagccagg ccgcaggcga tgggcgcggc gatagaaccg
     6841 atattgcccg cagcatatag cagcgaaaaa ccgccatcac gtcgatgatc gttctcgtcg
     6901 tagagctcgc caagcaaaca gctgatgttt gatttgaata aaccgtagcc acaaataatg
     6961 attgccagcg ccagatacag gctaaaggtt gaatttgtat caatgcccag caccacatgg
     7021 ccaagggtca ttaacagcgc gccggcaatc actgcagtgc ggttgccgag caggcggtcg
     7081 gcaagccagc cgccgagaat aggggtaacg taaaccagag aagcatatgc gctgaacagg
     7141 ctgatggcat ggttatcatc aaaaccaagc tgatgggtga gatagagaat gagtaaggca
     7201 cgcatgccgt aaaaactgaa gtactcccag atttggatcg ccacgatata gtatatcgcg
     7261 cgcggctgtg agggtgtttt catgtgttct ccttatgagc aaaaaaggga agtggcaagc
     7321 cacttccctt gtacgagcta attatttttt gctttcttct ttcaatacct taacggtata
     7381 gcggccatca gcctgacggt atgcaccgtg aatatcggtt tcaaagcccg gatagtgagc
     7441 gccgatttca cacagcatct gcaggaactc cagaaccgga cggctttctt cggtgatcat
     7501 ttcacccggc attaccagag gaactcccgg cggatacgga aggatcatat tggcgttaat
     7561 acgacctacc atttcgtcga ggtaaacttc ttcggtcata ccgtgcagct ctttctggaa
     7621 cgccgcatac ggagtcataa ccattgtcgg cagaacttca aatgcgcgat acatcagatc
     7681 cggcagattg tggtgaacaa tcagtttgtg gatattctga gccagttcct gaatacgcat
     7741 atcttcatag aattcaggat cttcacgata tagagacggc agcatgtttt tcacacgcag
     7801 gttcaggtcg aacgcacgtt tgaagtcagt cagagcacgc agcaggctca atgctttggt
     7861 cttatcaata ccgatgctga acaggaacag caggttatac gggccggttt tctcaacaac
     7921 gatgccatgt tcgtcgaggt atttcgccac gatgctggcc ggaataccaa agtcgctcat
     7981 ggtgccgtct ttttccatcc ccggggtcag cagggtgact ttgatcgggt caagatacat
     8041 gtgctcattg tcgatgttct tgaagccgtg ccaggagctg tcagaacgca gcggccagca
     8101 ttcagtggta tcgatatgat ccggctgcca tacatcaaag aaccagccat cagattccgt
     8161 tctcagacgt ttgatctctt tacggaactt gatcgcacgt tcaatggaac cgttgatcag
     8221 acgcttacca gcattacctt tcatcatcgc cgcagcggtt tcagtggacg ccacgatacc
     8281 gtagtgcgga gaggtggtgg tgtgcatcat gtaggcttcg ttaaaggttt cttcgtttac
     8341 gtcaccttta acgtggatca tggaagcctg agagaacgcc gccagcagtt tgtgagtgga
     8401 ctgggtttcg taaatcactt tcccttctac acggccaccg ctcataccgc atttaccttc
     8461 gtaaatcggc gagaagttgg tgtaaggcac ccacgcggag tcaaagtgga tggatttcac
     8521 atccagcgtt ttcttgatga agtcggtgtt gtacagcaga ccatcatagg tagagttggt
     8581 aattacggca tgtaccggcc aggttgcgtt tggtgtttct ttaacgcgct tagcaatggt
     8641 tgcgtgctgg aattcactct gtgggatacc accaagaata ccgtaagcgt tacgggtcgg
     8701 gcggaaatag attggggtaa catcgctcat catcattagg tgggttagcg atttgtggca
     8761 gttacggtca atcagaatgg tgctgcctgc cggagcagag tacataccga caattttgtt
     8821 cgcagtggaa gtaccgttgg tcaccatgta gctgcggtct gcgttaaaga cgcgagcgat
     8881 atactgttcc gcttctttgt gtggaccgct gtgatccagc agagaaccca attcagatac
     8941 tgaaatggaa atatcagatt tcatggtgtt cggaccaaag aaatcataga acaggctacc
     9001 taccgggctt ttctggaatg cagtaccgcc catgtgacca ggagtacaga aagtatattt
     9061 accttcacga acatatttaa acagtgcttt ggtcagcgga ggcagaatag tgttgatata
     9121 ttcgtcagtg gtctgcttga tcttgttagc aatatcatca gcagcaccca gcgcatattc
     9181 aaagaagcta atctgtaaac gcagatcgtt caggcttaca tcgagtgtgg aatacgtatt
     9241 agcgaacgcg tacaacggca ggttctcgtt cattttgcta atttcttcgc acagctcgag
     9301 attatattta tcccagtcaa aaataacacc gcacagacgc gcattgtttt cgatcagttt
     9361 taataagtcg tcacggtcat tcgggtaaac aatctggaag ttcagacgtt caagcgcgcg
     9421 atgaagttca cggatgggtt cttctttaaa ataaacaccc atgtgattca atattgcaat
     9481 aacgttcata gtcatatctc caggtaaaaa aggcccctcc caacacatgg gacaaaatga
     9541 aaggaggagc ctcggaaaat acttttaatt aatgtgcgtt agatgctgtg tggttatcca
     9601 ttgagtggct ctggcgctcg tgcattttgc gagcgtagaa catcaggata atcaggctga
     9661 cgatgaaggt accagccagc tcgaaggagc ttgcgcccat cagagcgatg aagcagaaca
     9721 cgcaacccag tacagagcag atcaggctga caaagttgcg gatgttaacg ccttcaaaac
     9781 gaatcaggtc aacgcaagag tagaagtacg gcagcatagt cagcagtact gcgataccgg
     9841 tcagttcacc gaacaggtca gatgctttac caccggcaga gttcatcaga gtgatgagga
     9901 tcatcagggc agtcattttc actgcagcca gcagcagacc ttttttcgga ataccgttgc
     9961 tgtcgacttc accataaact ttcgggaagt taccgtcgtt agcggcacgc acacctgcct
    10021 ggccgaccag catcatccag gagcccagag aagtcaggca cgcaaaagcg gtgaatgcag
    10081 aaaccatcgg cgcagcccag ttacccagga tagtagaagc actgattgca aacggagcac
    10141 cggagcctgc cattacagaa gacggataca taccggaaag aacctgagtc gcagcaatgt
    10201 aaacaatacc tgctaaacca gtccccagca tggttgccag tggaacagta cgtttcgggt
    10261 ttttaaccat accagtactt acagctgcgg attccacacc cacgaaggcc cacaggcaga
    10321 gcagaatact tttaatgatt gcatgaccat cagtagtacc tgcggtattc cagttagctg
    10381 cataagttgc agcatcaaac caatgccagc caacaatagc agtcataacc acaggaataa
    10441 gaaccagtac caggccaata gtggttaaac ggcttaccca ggtaccgccg agcatattta
    10501 caaaggtaaa tacccagacg atagcaatac aggcgatacc cgccggaaca ggatcattta
    10561 atactgggaa gaaggtggaa agataagata cagcggtaat accaattgcc aggttaccaa
    10621 tccagttagc atggtaataa agaacacctg tctgaaaacc aaatgcagga gaaatttctc
    10681 cggcatacgc aattgggcca ccttgttgcg ggttttttgt tgccagtcgg gcatatacat
    10741 atgccagcga cattgcacca ataatagaga taatccaacc ccagatagca ataccaccga
    10801 tacttgctag gttcgcaggt aataatgcaa taccgctccc catcatatta ccggcaacaa
    10861 caccggtaca ggcaaatagc ccgatcttct tggcagaact catgctcttc tcctaatttc
    10921 atttttgaat ttggagtccg ggtcatgatg tataactatt tcctgaccag accaaactgg
    10981 cgctaagatt actcacgaaa aaaggattaa tcctaaagat taggtgaaat aacacaaaag
    11041 tttctgtaag tgagaacttg aggtttttta ttaacacatc aggatcgcaa gttgatatca
    11101 tgaaaagata aacattcaat gtttacaatg tgttatgtga cattttctgg ttaaatttat
    11161 gtaataaaaa ttatgcggca aataaattgc cgcaacatat tataccaaca ggaacataca
    11221 aaaactcaac aacaaatatt cccgagcatg aatcaacccg ggagttactt attctgaagc
    11281 gagaaatttg tcgagataag gtacaacata aggaacagaa gtctggaata taccattttc
    11341 aatccagtaa agggtgtttg cccctgggcg taaattaaag gcggtgagat atgcatcagc
    11401 tgcttcccgg ttcatcccct tcatttcata aaccttgcca agcaacacat aatttagcca
    11461 ggacatttca agatcaatgc cagtatttat cgcctgataa gactcatctg ttttaccttt
    11521 taccagggca ctgaccgctt ttatttgata tataatggac aggttgttca attccggcag
    11581 tgtaacaatg ttatctattt ctgtgttaag tgctgctaat tgtttttcgt ctaaaggatg
    11641 ttgagaatgg cgcacgatat caactaatgc tttttctgct ctcgcgtagg taaattctgg
    11701 ggatgattga acaatctcac ctaataattc actggcacga tccaatgatt tatcatcacc
    11761 atgcagtaaa taatcatgtg cctgataaaa attagttaat aacgcaccac gatgcggcaa
    11821 aatttgctgg agcatctcct gcattcgttg tggccacggt tggtttaacg cttttgataa
    11881 actctccagt aaatcatttt gaatcgccag ctgattaccg ttagtgatga cataacgttt
    11941 atccagcatg gttgaaccat ctgcattgtc taccaatttt accgacataa agcattgttg
    12001 agcacggtat tggcgctgat taacaaacgc aatagataat gttttaccgg aactgctcgg
    12061 ttcatcaatg ttgtagttga ttttgtcatg caccataaag gtggagaagg tgttaagtga
    12121 tgtcgccacc aaatcaccca cgcctatcgc gtaagagagc tgatacgggg aactccagct
    12181 gttacaactt ttatttacca tattaatgtc aatatcgcgt ggattgagca aaatacgcga
    12241 tttgctcatc ggaagacgtg tatcaagact tgaaaacgct accagtgcta cacagatacc
    12301 taacgacaac aggaaaaaaa accataccca aaaggtagtg aatcgtttgc ttttaactgg
    12361 ggattgttca ggtggcgttg cggtgttttg aatgttaaga ctgtgggagg gagaatctgt
    12421 ggcaggaacc gcctctggta tagggggagg cgaagatagc attatttcct ctccctcttc
    12481 ttcgctgtac cagataaccg gcaccattaa tttatagccg cgctttggta cagtagcgat
    12541 atagacagga ctatcttcat cattatcttt taatgactta cgtagttctg agatactctg
    12601 cgtcacaacg tgattggtga caatacttct cttccagaca ttatcgataa gttcatccct
    12661 gctaagtact tcgccactgt gttgagcaaa gaaaaccaga aggtcgatta atctcggctc
    12721 aagggtaagt tgacgcccat tgcggctaat ttggtttatg gacggagtaa caagccattc
    12781 gccaacgcga actacaggtt gttgcataat agaaactcat tcgaaaaggg aatggtgcaa
    12841 tgataattgg cataacctat ttttaccatc tatagatggg tttatttaca tattattggt
    12901 gaatgcaaga cgttattttt accagacata aacttcttga ttacatagtg ttgagaaagg
    12961 attttactga gaaccagaag taatattcct taccatcaaa attcatcatc tttgccaaag
    13021 aaaaatgttc agaaaataat ccatggaaaa ttgtccggag cgcttactat tttaatggat
    13081 tgttagtctt tgcatgagca aacggactga tacatttctc tttgttctca ttcagaaaat
    13141 ctcatcagtt ggcgttctgc ccgatgttgt gctttatggg tgcgttacgc tcctgatgac
    13201 gtcatttgac gttcaacagc atcacggggc cgcacgacat ttcacgtcag tcagcgctat
    13261 agctcagaaa caaattttcc cgaattggaa tatgcccgca aattgctggg gatgtgggga
    13321 gaatctggtt gagttcggta gaattgattt ggcggcagga cgcttaaatc gtggcgtcct
    13381 gaaacgaaaa acggacctcc gtggaggtcc gtttatatga atttggtgcc ggactcggaa
    13441 tcgaagattt catttatcat attgatatta atctcgttta tttggtttga tgaaaaatgt
    13501 accctctctt gtaccctcgg tcccttctgg tggtttttat gagaacaagt agatcaagat
    13561 ttttgtcatc agaacacgtg tatttttgtg ggttttattc ctgagtttta ttccttttta
    13621 tatcgctaac gctccgggat agtgcttagc aataatatca ttaagcctgt caaccagctc
    13681 tggtttcctg aacgtgatgt gcgcggagcc cttcttaaag tatctgatgc tgaacatctc
    13741 atcttcatag tcctctttac cctgaacaga ctggacgtga tcatccagat gaatagtgat
    13801 gttttcccgg ttgtcgggga tcggtttgcc gctgaacagg tgtagcatta tttccagatc
    13861 agcaagtcga tctgcctgtt gcccggtgat aagatgaaag ccccaccggt cccacctcac
    13921 caggttgttg acgataattt tactgccaaa tttgcagggg caattggttt tgtaattcca
    13981 gctcaaccct ctgaagacgt tgatcacccc ccgctcaaac acctcatcct tattctggtg
    14041 cagttgttca aatgtgctca gaatgttcgc ttcactgatt tccggaaaat tatcgtattc
    14101 cagtgaccgg taccatgtat cacgggcctg tgcatccatt aaggaaagca tcccggaacg
    14161 ttgcatcagg tcacgccaga tttcgcgatc gagattgcgt gtgatggctt tcattgcggt
    14221 ttcaggtttc tccatcagcc agcaaccgca gtgaaaattc tgtttcatgg cccagtccag
    14281 tgcggtttta ccgccaatgc tttctgtcat tgctgagatt tcagccagtt gccggataag
    14341 tgtgtcgatt tgcagcagtg tactctttcg tacagccaga atgtggagga tactggttga
    14401 gcagataacg tccgtatgcc agttgacaac ttccggacgg gagactgtga tatcagacat
    14461 aatcgatatt cctttttgca gataaatgaa aacgccagcc ctgttcaggc tggcgtcagt
    14521 ggaatgaagt ctggtgagtg agagtcaccg gttctggtgc agaagctgca actggcgcag
    14581 tgtacggata tcttccctgg cctgttcaat gcagggcagc agtgctgccg aatccgtttt
    14641 atcctcatcg ttgtcaaaga taattcccga ttcgcagtcg atattgtcct gcagccacgc
    14701 aatcagaata tccagcgctg tttccgtggt taatgatttc atgttgtttc tggttaccag
    14761 tcgaaagtgg gtaaacccgg caggcattca gcgctggcat ccagatgaat gagactgacg
    14821 ccataacgtc gggtaagtgt gacaaccaga cggcggaacg tttttgacag ccccaggcgt
    14881 ttaagccgca gaaccgggta tgaccacgca tcggtacgta acagatatcc gctacctgtg
    14941 aaatgaatcc attctgattc gccggtgtca ttcgtctgat gtgacagcga gtactgatct
    15001 tacccagcaa tagtggacac gcggctaagt gagtaaactc tcagtcagag gtgactcaca
    15061 tgacaaaaac agtatcaacc agtaaaaaac cccgtaaaca gcattcgcct gaatttcgca
    15121 gtgaagccct gaagcttgct gaacgcatcg gtgttactgc cgcagcccgt gaactcagcc
    15181 tgtatgaatc acagctctac aactggcgca gtaaacagca aaatcagcag acgtcttctg
    15241 aacgtgaact ggagatgtct accgagattg cacgtctcaa acgccagctg gcagaacggg
    15301 atgaagagct ggctatcctc caaaaggccg cgacatactt cgcgaagcgc ctgaaatgaa
    15361 gtatgtcttt attgaaaaac atcaggctga gttcagcatc aaagcaatgt gccgcgtgct
    15421 ccgggtggcc cgcagcggct ggtatacgtg gtgtcagcgg cggacaagga taagcacgcg
    15481 tcagcagttc cgccaacact gcgacagcgt tgtcctcgcg gcttttaccc ggtcaaaaca
    15541 gcgttacggt gccccacgcc tgacggatga actgcgtgct cagggttacc cctttaacgt
    15601 aaaaaccgtg gcggcaagcc tgcgccgtca gggactgagg gcaaaggcct cccggaagtt
    15661 cagcccggtc agctaccgcg cacacggcct gcctgtgtca gaaaatctgt tggagcagga
    15721 tttttacgcc agtggcccga accagaagtg ggcaggagac atcacgtact tacgtacaga
    15781 tgaaggctgg ctgtatctgg cagtggtcat tgacctgtgg tcacgtgccg ttattggctg
    15841 gtcaatgtcg ccacgcatga cggcgcaact ggcctgcgat gccctgcaga tggcgctgtg
    15901 gcggcgtaag aggccccgga acgttatcgt tcacacggac cgtggaggcc agtactgttc
    15961 agcagattat caggcgcaac tgaagcggca taatctgcgt ggaagtatga gcgcaaaagg
    16021 ttgctgctac gataatgcct gcgtggaaag cttctttcat tcgctgaaag tggaatgtat
    16081 ccatggagaa cactttatca gccgggaaat aatgcgggca acggtgttta attatatcga
    16141 atgtgattac aatcggtggc ggcggcacag ttggtgtggc ggcctcagtc cggaacaatt
    16201 tgaaaacaag aacctcgctt aggcctgtgt ccatattacg tgggtaggat cactactcat
    16261 actataagag ggtccacttc cccccggata tatgatgatg gatggagtat ccggagccca
    16321 gcatgctcca ttagcatcaa tatatgttcc catatctttt ccacgtagat ctattacatt
    16381 agtagaacca ggtaatccat taagattcat ccctcctttc acggaatcaa gcatgttatc
    16441 tgatatttct ctcattttaa aaacctcctg ctcatttatg ttcagatatt aatttggtgt
    16501 tgcaaccatt tattatcaca gaattcagaa tatcattcag ttttattttt gttaaaatat
    16561 cccctcttaa gacagacaca tattcttgtt tatcattttc attaaacaat gactctatat
    16621 tttctacgtt taaataatat agcccacaag cagcataaat tttagactcc ttagttgact
    16681 ttctattttt tattatattt ttaaatatct cttcgggatt atctaatgta agaatgtgtg
    16741 tgtataattt ttccccttcg ctctgatagc ctataaaccc attcatacct atagaaaaat
    16801 aggttgtttt ttctaacgat tgttgaaggg gaggtgga
//
LOCUS       DAZGQL010000109        16830 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.109, whole genome shotgun sequence.
ACCESSION   DAZGQL010000109 DAZGQL010000000
VERSION     DAZGQL010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16830
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.109"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(24..719)
                     /gene="araD"
                     /locus_tag="ACHKIR_004163"
     CDS             complement(24..719)
                     /gene="araD"
                     /locus_tag="ACHKIR_004163"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFO9991826.1"
                     /translation="MLEDLKRQVLEANLALPKHNLITLTWGNVSAVDRERGVLVIKPS
                     GVDYNVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            complement(919..2421)
                     /gene="araA"
                     /locus_tag="ACHKIR_004164"
     CDS             complement(919..2421)
                     /gene="araA"
                     /locus_tag="ACHKIR_004164"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFO9991827.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGIKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            complement(2432..4132)
                     /gene="araB"
                     /locus_tag="ACHKIR_004165"
     CDS             complement(2432..4132)
                     /gene="araB"
                     /locus_tag="ACHKIR_004165"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HFO9991828.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCATGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSAEQRAAVVGIGVDTTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHTPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIANKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKEQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVPTL
                     "
     gene            4471..5349
                     /gene="araC"
                     /locus_tag="ACHKIR_004166"
     CDS             4471..5349
                     /gene="araC"
                     /locus_tag="ACHKIR_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HFO9991829.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAAKLS"
     gene            5435..6199
                     /gene="yabI"
                     /locus_tag="ACHKIR_004167"
     CDS             5435..6199
                     /gene="yabI"
                     /locus_tag="ACHKIR_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFO9991830.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLAPLISAVGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(6313..7011)
                     /gene="thiQ"
                     /locus_tag="ACHKIR_004168"
     CDS             complement(6313..7011)
                     /gene="thiQ"
                     /locus_tag="ACHKIR_004168"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HFO9991831.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERSEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGMTDELL
                     SGKASASAILGITG"
     gene            complement(6995..8605)
                     /gene="thiP"
                     /locus_tag="ACHKIR_004169"
     CDS             complement(6995..8605)
                     /gene="thiP"
                     /locus_tag="ACHKIR_004169"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HFO9991832.1"
                     /translation="MATRRQPLIPGWLIPGISAATLVVAVALAAFLALWWNAPQGDWV
                     AIWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLWQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALIQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVLVDGINRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            complement(8581..9564)
                     /gene="thiB"
                     /locus_tag="ACHKIR_004170"
     CDS             complement(8581..9564)
                     /gene="thiB"
                     /locus_tag="ACHKIR_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HFO9991833.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWKNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     regulatory      complement(9595..9694)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(9728..11383)
                     /gene="sgrR"
                     /locus_tag="ACHKIR_004171"
     CDS             complement(9728..11383)
                     /gene="sgrR"
                     /locus_tag="ACHKIR_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HFO9991834.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAA
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQILPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     SSNQLKIQAFDDFFGYRALIDEVNVWVLPEIADDPAGGLMLKGPQDEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWISYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHTRT
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEINLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     gene            11472..11603
                     /gene="sgrT"
                     /locus_tag="ACHKIR_004172"
     CDS             11472..11603
                     /gene="sgrT"
                     /locus_tag="ACHKIR_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046325 - negative regulation of glucose
                     import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HFO9991835.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEELMQWEGSHSDY"
     gene            complement(11711..12316)
                     /gene="leuD"
                     /locus_tag="ACHKIR_004173"
     CDS             complement(11711..12316)
                     /gene="leuD"
                     /locus_tag="ACHKIR_004173"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HFO9991836.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVQANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIASYEEKQPAFMR"
     gene            complement(12327..13727)
                     /gene="leuC"
                     /locus_tag="ACHKIR_004174"
     CDS             complement(12327..13727)
                     /gene="leuC"
                     /locus_tag="ACHKIR_004174"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HFO9991837.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            complement(13730..14821)
                     /gene="leuB"
                     /locus_tag="ACHKIR_004175"
     CDS             complement(13730..14821)
                     /gene="leuB"
                     /locus_tag="ACHKIR_004175"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HFO9991838.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAACAIERAINRALEEGI
                     RTGDLARGAAAVSTNEMGDIIARYVVEGV"
     gene            complement(14821..16392)
                     /gene="leuA"
                     /locus_tag="ACHKIR_004176"
     CDS             complement(14821..16392)
                     /gene="leuA"
                     /locus_tag="ACHKIR_004176"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HFO9991839.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            complement(16485..16571)
                     /gene="leuL"
                     /locus_tag="ACHKIR_004177"
     CDS             complement(16485..16571)
                     /gene="leuL"
                     /locus_tag="ACHKIR_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HFO9991840.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRLVSGIQN"
BASE COUNT         3711 a         4583 c         4584 g         3952 t
ORIGIN      
        1 ttggctgtgg ttttatacag tcattactgc ccgtaatatg ccttcgcacc atgcttacgc
       61 agatagtgtt tatccagcag cgtttgctgc atatccggta actgcggcgc taactgacgg
      121 cagaatatcc ccatataagc gacctcttcc agcacgatgg cgttatgcac cgcatcttcg
      181 gcatttttgc cccatgcaaa tgggccgtga gaatggacca ggacgccggg catttgtgct
      241 gcgtcgattc cctgtttttc gaaggtttct acgatgacgt taccggtttc ccactcatat
      301 tcaccgttga tttctgcgtc ggtcattttg cgggtgcagg gaatggtgcc gtagaaatag
      361 tcggcgtggg tggtaccggt tgccggaatt gactggcccg cctgcgccca gatggtggcg
      421 tggcgcgagt gtgtgtgcac aatgccgcca atggacggga atgcctgata gagcagccgg
      481 tgagttggcg tgtcggagga gggctttttc gttccttcaa ccacttcacc ggtttcgatg
      541 ctgaccacga ccatatcgtc agcggtcatg acgttgtaat cgacgccgga aggtttgatc
      601 accaagacgc cgcgctcgcg atcaacggcg ctgacattgc cccatgtgag cgtgatcagg
      661 ttatgttttg gcagcgccaa attggcttct aatacctggc gtttgagatc ttctaacatg
      721 ttgactcctt cgtgccggat gcgctttgct tatccggcct acaaaatcgc agcgtgtagg
      781 cctgataaga cgcgtcagcg tcgcatcagg cggtgaatgc cggatgcgct ttgcttatcc
      841 ggcctacaaa atcacagcgt gtaggcctga taagacgcgt cagcgtcgca tcaggcgtta
      901 cacacctgat gcggctactt agcgacgaaa cccgtaatac acttcgttcc agcgcagcgc
      961 gtctttaaac gctggcaggc gggtatcgtt atcaatcacc gtgatttcaa tgtcgtgcat
     1021 ctcggcgaac tggcgcatat cgttgaggtt cagcgcatgg ctgaaaacgg tatggtgcgc
     1081 gccaccagca aggatccacg cttcggaagc agttggcaga tccggttgcg ctttccacag
     1141 cgcattcgcc accggcagtt tcggcaggga gtgcggtgtt ttcaccgtgt cgatgcagtt
     1201 aaccagcaga cggtaacgat cgccgagatc aatcaggctg gcgacaatcg ctggaccggt
     1261 ttgggtattg aagatcaggc gggcaggatc gtctttaccg ccaataccga ggtgctgaac
     1321 gtcgaggatc ggtttctctt ccacagcgat cgacggacag acttccagca tgtgggagcc
     1381 gagcaccagg tcattgcctt tctcgaagtg ataggtgtag tcctccataa aggaggtgcc
     1441 gccctgcaga ccggttgaca tcaccttcat gatgcgaagc agggcggcag ttttccagtc
     1501 gccttcgccc gcaaagccgt aaccctgctg catcagacgc tgtacggcca gaccaggaag
     1561 ctgtttcaga ccgtgcaaat cttcaaaggt ggtggtgaac gcgtggaagc caccttgttc
     1621 caggaaacgc ttaatcccca gctcaatacg cgccgcttcc agcacgttct ggcgtttttc
     1681 gccgtggatt tgtgtggcag gcgtcatggt gtagcagctt tcgtactcat cgaccagcgc
     1741 gttaacatcg ccatcgctga tggagttcac tacctgcacc agatcgccaa ccgcccaggt
     1801 attgacggag aaaccgaact tgatctgtgc ggcaacttta tcaccatcgg tgaccgccac
     1861 ttcacgcatg ttatcgccaa aacggcagac tttcagatga cgggtatcct gtttagagac
     1921 cgcctgacgc atccaggagc cgatacgctc atgggcttgt ttatcctgcc agtgaccggt
     1981 aacgacggca tgttgctgac gcatacgcgc gccaatgaag ccgaactcgc gaccgccatg
     2041 tgcagtctgg ttcaggttca taaagtccat atcgatgcta tcccacggca gcgccgcgtt
     2101 aaactgggtg tggaattgca gcaacggttt gttgagcata gtcaggccgt tgatccacat
     2161 tttggccggg gagaaagtgt gcagccacac taccaggcca gcgcaacgat cgtcgtaatt
     2221 cgcgtcacgg caaatagcgg tgatttcatc cggcgtggtg cccagcggtt tcagcaccag
     2281 tttgcagggc agtttcgctt ccgtattcag cgcattaacg acgtgctcgg catgttgggt
     2341 gacctgacgc agggtttccg ggccatacag atgctggctg ccaattacaa accacacttc
     2401 ataattatca aaaatcgtca ttgtcgtgtc cttatagagt cggaacggcc tgggcagcct
     2461 gtgccggggc ggaagttgga agatagtgtt gttcggcgct catcgcccat tgctgatagc
     2521 ggcgataaag ctgttcaaag cgttgtgcct gttcgctgcg cggttgcagg gttttctcta
     2581 ccgcactggc cattttttgc tgggctgagg ggatgtctgc gtgcactttc gcggcgacgg
     2641 cagcgaaaat cgccgcaccg agcgcacagc actggtcaga ggcaacaatt tgcagcgggc
     2701 gattcagcac gtcgcagcag gcctgcataa tgacctggtt tttccgcgcg atgccgccca
     2761 gcgccatcac gttattgacg gcgatcccct gatcggtaaa gcactccatg attgcgcgtg
     2821 cgccaaaggc ggtggcagca atcaatccgc cgaacagcag cggagcgtcg gtagcgaggt
     2881 tcagatcggt aatcacccct ttcaggcgtt ggttagcgtt tggcgtgcgg cggccgttaa
     2941 accagtcgag caccaccggc aggtgatcca gagacggatt tttggcccat gcttcggtca
     3001 gcgccggaag cagttgtttc tggctggcgt tgatttgctc tttcagttcc ggatgctggg
     3061 cggcaagctg ttccagcggc cagccgagta cgcgaccgaa ccaggcgtag atatcaccaa
     3121 acgccgattg gcctgcttcc agaccgataa atccaggcac cacgctgcca tcaacctgac
     3181 cgcaaatacc tttcactgcc cgctcgccaa cgctctgttt gttggcaatc agaatgtcgc
     3241 aggtggaagt accgataact tttaccagag cgttaggctg tgcgcctgcg ccaactgcgc
     3301 ccatatggca gtcaaacgcg ccgccggaaa tcaccacgct ttcaggtagg ccgagacgct
     3361 gcgcccattc cgggcataag gtgcccaccg gaatatcggc agtccaggtg tcagtgaata
     3421 gcggggaagg caaatggcga ttgaggatcg ggtccagctc atcaaagaaa ctggctggcg
     3481 gcaggccgcc ccagctttca tgccatagcg acttatgccc ggcgctgcaa cgtccgcgac
     3541 gaatatcctg cgggcgggtg gtaccggaaa gcagagctgg cacccagtcg cacagctcaa
     3601 tccacgatgc ggcagattgc gccacggcgc tgtcctggcg agtcacatgc agtatttttg
     3661 cccagaacca ttcgctggaa taaataccgc caatatagcg ggagtagtca acgttgcccg
     3721 gcgtgtgaca taagcgggtt atctcttccg cttcttcaac cgcggtgtgg tctttccaca
     3781 atacgaacat cgcgttcggg ttttcggcaa actccgggcg cagcgccagg acattaccgt
     3841 ccgcgtcaat cggtgcgggc gtcgagccgg ttgtgtcaac gccaatcccg accacagctg
     3901 cgcgctgctc ggcgctaagc tctgcaagca cggttttcag cgccgcttcc attgactcaa
     3961 tgtagtcacg cggatgatga cggaactggt tatttggggc atcacaaaat tgccctttct
     4021 gccaacgggg ataccactct acgctggtgg cgatctcttc accggtggcg cagtccaccg
     4081 ccaaagctcg cacagaatca ctgccaaaat cgaggccaat tgcaatcgcc atcgtttcac
     4141 tccatccaaa aaaacgggta tggagaaaca gtagagagtt gcgaaaaaaa gcgtcaggta
     4201 ggatccgcta atcttatgga taaaaatgct atggcatagc aaagtgtgac gccgtgcaaa
     4261 taatcaatgt ggacttttct gccgtgatta tagacacttt tgttacgcgt ttttgtcatg
     4321 gctttggtcc cgctttgtta cagaatgctt ttaataagcg gggttaccgg ttggattagc
     4381 gagaagagcc agtaaaagac gcagtgacgg caatgtctga tgcaatatgg acaattggtt
     4441 tcttctctga atggcgggag tatgaaaagt atggctgaag cgcaaaatga tcccctgctg
     4501 ccgggatact cgtttaacgc ccatctggtg gcgggtttaa cgccgattga ggccaacggt
     4561 tatctcgatt tttttatcga ccgaccactg ggaatgaagg gttatattct caatctcacc
     4621 atacgcggcc agggggtggt gaaaaatcag ggacgagaat ttgtctgccg accgggtgat
     4681 attttgctgt tcccgccagg agagattcat cactacggtc gtcatccgga ggctcgcgaa
     4741 tggtatcacc agtgggttta ctttcgtccg cgcgcctact ggcatgaatg gcttaactgg
     4801 ccgtcaatat ttgccaatac gggtttcttt cgcccggatg aagcgcacca gccgcatttc
     4861 agcgacctgt ttgggcaaat cattaacgcc gggcaagggg aagggcgcta ttcggagctg
     4921 ctggcgataa atctgcttga gcaattgtta ctgcggcgca tggaagcgat taacgagtcg
     4981 ctccatccac cgatggataa tcgggtacgc gaggcttgtc agtacatcag cgatcacctg
     5041 gcagacagca attttgatat cgccagcgtc gcacagcatg tttgcctgtc gccgtcgcgt
     5101 ctgtcgcatc tgttccgcca gcagttagga attagcgtct taagctggcg cgaggaccaa
     5161 cgcattagtc aggcgaagct gcttttgagc accacccgga tgcctatcgc caccgtcggt
     5221 cgcaatgttg gttttgacga tcaactctat ttctcgcgag tatttaaaaa atgcaccggg
     5281 gccagcccga gtgaattccg tgccggttgt gaagaaaaag tgaatgatgt agccgccaag
     5341 ttgtcataat tggtaacgaa tcagataatt gacggcttga cggagtagca tagggtttgc
     5401 agaatccctg cttcgtccat ttgacaggca cattatgcaa gcattgctgg aacactttat
     5461 tacccaatcc accgtgtatt cattgatggc ggtggtgttg gtagcctttc tggagtcgct
     5521 ggcgctggtc ggtttgattc tacccggtac ggtgctaatg gcgggactgg gggcgctgat
     5581 tggcagtggc gagttaagtt tctggcacgc ctggctggca gggatcgtcg gttgtctgct
     5641 gggcgactgg atctccttct ggctgggctg gcgttttaaa aagccgttgc atcgctggtc
     5701 atttctgaag aagaacaaag cattactcga taaaaccgaa catgcgctgc atcagcacag
     5761 catgttcacc attctggtcg gtcgctttgt tggcccgacg cgtccgctgg tgccaatggt
     5821 ggcgggaatg ctggatctgc cggtggcaaa attcattacg ccgaatatta tcggctgcct
     5881 gctgtggcca ccgttttact tcctgccagg gattctggcg ggtgcggcga tcgatattcc
     5941 agccggaatg cagagcggcg agtttaaatg gttgctgctg gcgacggcgg tatttttgtg
     6001 ggttggtggc tggctgtgct ggcggttatg gcgcagcggt aaagcgactg accgtttgag
     6061 tcattatttg tcgcgcggtc gtttgttgtg gctggcaccg ttaatttctg ccgtcggtgt
     6121 agtggcgctg gtggtgttaa ttcgccaccc gttgatgccg gtgtatatcg atattttgcg
     6181 taaagtggtt ggggtttagg ggatagcctt gtgcgggttg cctgatgcga cgcttgccgc
     6241 gtcttatcag gcctacaaaa cgcactgtcc gtaggtcgga taaggcgttc acgccgcatc
     6301 cgacagtgca tactaacccg taatccccaa tattgccgaa gcactcgcct taccgctcaa
     6361 caactcatcg gtcataccct gccaggcgat gcgcccgtcg gcgaccacca ccgagcgcgt
     6421 ggcgatccgc gccgcatctt ccacactgtg cgacaccatc aatagcgtca ttttttgctg
     6481 ttggcagctc gtgctcacca gcgtcaacat ctcctgacgt aacgccggat cgagcgcaga
     6541 gaacggttca tcgagcaata aaatcggctg ttcgcgcacc agacaacgcg ctaacgccac
     6601 gcgctgtcgc tgaccgccag aaagctcgcc cggtaagcgc gccattaaat tatcaatccc
     6661 catctgacgg gcaatcgcgt gcattttctc ctgttgtgcc gcgttcagct tcaatcctgg
     6721 attaagcccc agcccgatgt tctgcgcgac cgtcaggtga ctgaacaggt tgttttcctg
     6781 aaacagcatc gacaccggac ggcgtgacgg cggcgtcgtg gtgtgatcaa cgccgtcgat
     6841 agtcagcgaa ccgctggctg gcgtcagaaa accggcgatc aaattcagca gggtactttt
     6901 acccgcgccg cttggcccga ggatcgccac ctgctcgctg cgttccaccg ttaagctaaa
     6961 acgcatcggc aaatggtggt aaagccaggt gatatcagtc agttttaaca tttcgccccg
     7021 gtagtttttc aatcacggta aacagcagaa aacagagtag cagcagaatt aacgcggtga
     7081 ccgcgccgtc ctggctgcga taggagccaa tttgctggta gagataaaac ggcagggtgc
     7141 ggaaatcatc gttaccaaac aacgccacca cgccaaaatc accaatcgat agcacacaag
     7201 caaaggccag tgcctgcgcc agtgggcgtt taagggcgcg cagctccacc acttttaagc
     7261 gtgaccagcc ttcaatgccc agcgactgac acaacatact gtaacgcgcg gtgatatcgc
     7321 gcatcgggtt ttccagcact ttcagcgcat aagggatcgc cattaacgca ttggtgaaaa
     7381 tcacaatgcc gtcagcagat tgtggcaggc cgatagtgtt gttgagcagt aaaaagaagc
     7441 cggtagccag cacaatcccc ggcatcgcga ggatcaacat gccgctcatc tccagcgcct
     7501 gacccgccag cattttctgc cgcgcccgca gttcgcgact gctccatagc agcatcatgg
     7561 tcagcactac gcataatacg cctgccgcca gcgcaatacg caacgaggtc cacagcgcct
     7621 gccacagcac cggctgcgcc agcacttccg gcaactggcg atttatccca tcgacgagca
     7681 ccgccagtaa cggtggcagc aacagcaaca gcgccagcac aattaacacc gtgtcgcaaa
     7741 tgcggctatg cagacgatcg tccgggtcgc gccagccttg cagcagcgta gtgccgggcg
     7801 caatggcctt actcaatcgc tgactcaaca gcaccagccc aaggcaacac accatctgga
     7861 tcagcgccag catcgccgcg cgggcaggat cgtagtcgta actcagcgcc tgataaatag
     7921 ccagctcgat agtcgtcgct tgcggaccgc cgcccagcga caacacggtg gcgaagctgg
     7981 cgaaacagag cataaagata agcgcggcaa ccggcgggat ttgtcgccgt aaccacggcc
     8041 attcgacgaa gcggaaaaaa tgccagccgc gcatcccaag ctgggcggca agctgacgtt
     8101 gttccccggg aatgttttcc agtgcctgga gtaataagcg gctcgccatc ggcagattaa
     8161 aaaatacatg cgccagcaaa atgccttgca ggccgtaggg cgaaaacgtc cactccagac
     8221 caagcgattg ccagagcgat gccagccagc cctggcgacc atagacgcta agaatgccga
     8281 aaacagcaac cagcaccggg aggatcaagg tcatggcaca cagacgcaac agcgccagcc
     8341 gtcctggaaa gcgcctgcgg tagagggcgc gggcaaggaa tatcgcgggc acgacagaga
     8401 gcagcgccga gagaaacgcc tgccagaagg agaagcgcac cacatgccac aggtagctgt
     8461 cctgccagat tgccacccag tcaccctgcg gcgcgttcca ccacagggcg agaaacgcag
     8521 ccagtgcaac cgccaccacc agcgtggcgg cgcttattcc tggaattaac cagccgggaa
     8581 ttaacggctg acggcgcgtt gccattcgct aatccatgcc tgacgttgtg ccgccacttc
     8641 ggctggcgtg aactccagcg tggttgccgg tttggtcaat tgttcaaaac cggcaggcag
     8701 cgtgacgttt gccaccggat acatccagtt gccggttggg atcgcattct gaaaagccgg
     8761 agaaaccata aactggagaa atttttgcgc cagctccggc tgcttgctgg cagcggtgcg
     8821 ggcggcgact tccacctgca gatagtgacc ttcgctgaag tttgcggcgg cgtagttatc
     8881 tttcttctct tcgagaatgt gataagccgg agaggtggtg taactcagta ccagatcgct
     8941 ttcacctttt aaaaacaggc cgtaggcttc gctccagcct ttggtgaccg tgaccgtttt
     9001 cttcgccagt ttctgccagg cttgtggggc gtcatcgcca tagacttttt gcatccacaa
     9061 cagcaggccc agccccggcg tactggtgcg cggatcctga taaatcaccc gccagttttg
     9121 atcgctctca acaagctctt tcaggctttg cggcggattt ttcagtttgt tcttgtcata
     9181 gacgaaggcg aaatagccgt aatcaaacgg tacgaaagtg tcatttttcc agccgccggg
     9241 aacgttaacg gcatccgctg ccacgccgct tttggcaaat agtccggttt tactggcggc
     9301 gtctaacagg ttgttatcca gccccagcac cacatcggct ttactgtttt tgccttccat
     9361 ccgcagacgg ttgagcagcg aaacgccatc ttccagcgcc accagtttca gttcgcagtt
     9421 acagtcggct tcaaaagctt ttttgatctt tggaccaggc ccccagtcgg cggcgaagga
     9481 atcgtaggta taaacagtca gaacgggttt agcgaaaacg ggcgctgtgc acagcaacag
     9541 caaggggaga cattttttta acactttgca cctcaaaaaa gagtggcaaa ggacttgaga
     9601 aggagcctca aatcccttcg ccggcgttat ccggatcagg ttcgacgggt attttctcag
     9661 cgcacgcgtc tgcgtggcac cccgttgaga acggcgttag tgtagtgatt ttgttatcaa
     9721 ccagcaatca tggatccggc ggcgcaaacc acgctgattt aaaatcgaac cagccgaggg
     9781 tattcatacg caggccgcgc atactgcgtt gcccctgaat gatcagccag tggtggataa
     9841 gcggcaccat tgctttgctg gcgaccagtt gctggcacca gttcgccagg tttatttcgc
     9901 cattgcgcca gcgggcggcg tcggcttgcc agtcgatcgg aatacagtgt tgtagcagcg
     9961 gcacctcgca caggtgcgcg aatagcgaaa aatccagcgg cagcgtaaag ttggcgctgt
    10021 taagccagat atcgctctcg atctctcctt catgccactg atcgtagctg atctctttga
    10081 tttccagcgt gacctggtga cttgccagaa tctgctgcat gatcccggca atcaccctat
    10141 gctcactgtg atcctgataa aaggtcaggg tgaggctttc cagaccagcc ggtttttcgc
    10201 tctttatggt gcgggtatgg tgccaacggg gaagcagccc ataggccgga aaccacaact
    10261 gctggtactg ttcctcggcg aaataaacca gattggtcgg cgaaagcaca tagcttatcc
    10321 agtctctgac ttgctgattc gccccgcgat gggtacggct gtcgaacagt aaatagtaac
    10381 aaccttcttc aaggcggctt tcaatctctt tttcctcgtc ttgcggtcct ttcagcatca
    10441 ggcctcccgc ggggtcatca gcaatttccg gcaagaccca gacgttaact tcgtcgatta
    10501 atgcccgata accaaagaag tcgtcgaatg cctgaatttt cagttgattg ctgctgttgc
    10561 ggatcaccgc ataaggaccc gttccgatgg gatggctggc aaagttgctg agggtttccc
    10621 attcgcgcgg caggatcatc gccggaactt gccccagcag taacggtaac cagcggtccg
    10681 gttgcgtgag atggatatcc agcgtccagg gcgtcggcga cacaatgtca gcaatatgcg
    10741 aatagagcgg cagcgtattg attcgtttta aagaggcgat cacgtcgtcc atttccagtt
    10801 cacgaccatg atgaaaatgg actcctggac gcaaaaagaa acgccagtga agcggtaaaa
    10861 tttgctgcca gtggtgggcg atgtccgctt ccagttcccc attttcctca tttatgcgcg
    10921 ttagcgaact gaagatttgc cgggcgatat gggtttcgga acgacgcaat gcactgccgg
    10981 gcagcagatt acgcaacgga cgatagtaga gaacgcgcag gatgtgccgc ccctggcgga
    11041 agctgcgtcc cagatgagaa accaacattt gccgcacagc cgctttgtcg ccaaccagtt
    11101 gcaccagttg atcgatacga tcctgctcca gcaggtcctc tgctcgctgt tgctgaagcg
    11161 ccagcccggt atagaggaat gtcagacgcg agcgtttgcc gcgcccgact tccgcttccc
    11221 acgtcagcca gccgcgatcc tgcatggtgt tgagcagggt gcgcatatga cgacgcgagc
    11281 agctcaataa cgctgccagt tcgttgagcg tcgtgtcctg cgatttaccc tcgcagcatt
    11341 gccacaggcg gatgaactgt tgttgcagac gagcagatgg cataaaaggg gaactcctgc
    11401 gcaaaagaca gcaattttat tttccctata ttaagtcaat aattcctaac gatgaagcaa
    11461 ggaggtgcct catgcgtcag ttttatcagc actattttac cgcgacagcg aagttgtgct
    11521 ggttgcgttg gttaagcgtc ccacaacgat taaccatgct tgaagaactg atgcaatggg
    11581 aggggagtca ttctgactac tgatttgcgg acatcatgtg tgactgagta ttggtgttaa
    11641 tcgccacgcc agcagtgatt atctgctggc ttttttcttt ttggcgattg agtgccggac
    11701 ggtgtacaaa ctaacgcata aacgccggtt gcttctcttc ataagacgca attgcgtcgt
    11761 cgtgctgcag agtcaggccg atgctgtcca gaccgttcat catgcagtgg cggcggaagg
    11821 catcgatggt aaagcgataa gttttctctc ccgctttcac ctcttgcgct tccaggtcta
    11881 catcaaaatg aatccccgga ttggcctgca ccagcgcaaa cagttcgtcc acttctgcgt
    11941 cgcttaattt caccggcagc agctggttgt taaagctgtt gccgtagaag atgtcagcaa
    12001 aactcggtgc aatcaccact ttaaaaccgt aatcggtcaa tgcccacggc gcgtgctcac
    12061 gcgaggaacc gcagccgaag ttttctcgtg ccagcaaaat ggaagcgccc tgatactgcg
    12121 ggaagttcag cacgaagtcc gggtttggct gttggccttt ttcatccaga aaacgccagt
    12181 cgttaaacag atgcgcgcca aaacccgtac gggtcacttt ctgcaaaaac tgtttcggga
    12241 tgattgcatc ggtatcgaca ttggcggcat ccagcggaac caccaggcct gtgtgtttga
    12301 taaatttctc tgccatggtg tgctccttat ttaatgttgc gaatgtcggc gaaatgtccg
    12361 gtcacagcag cagcggcagc cattgccggg ctgaccagat gggtgcgccc tccacgcccc
    12421 tggcggcctt caaagttacg gttgctggtg gaggcacaac gctcacccgg attcaggcga
    12481 tcgttgttca tcgccagaca cattgagcaa cctggcaagc gccattcaaa accggcttca
    12541 ataaagattt tgtccagacc ttccgcttcc gcctgggctt ttaccgggcc agagccggga
    12601 accaccagtg cctgcacgcc tggcgcgact ttccgtcctt tggcgatttc agctgccgcg
    12661 cgtaaatctt caatacgtga gttagtgcag gaaccgataa acactttgtc gatagccact
    12721 tctgtcagcg gaatacccgg tttcagcccc atataggcca gcgctttttc tgccgacgcg
    12781 cgttcaaccg gatcggcaaa cgaagccgga tcgggaatgt tgtcgttcac ggaaatcacc
    12841 tgacctggat tggtgcccca ggtgacctgc ggtgaaattt cttctgcctg caaagtcaca
    12901 acggtatcga aagttgcgcc ttcgtcggtt tgcagagttt tccagtaggc aacggcgtcg
    12961 tcgaaatctt tgcctttcgg cgcatgcaga cggcctttga catagttaaa ggtagtttcg
    13021 tccggtgcaa ccagaccggc tttcgcgccc atttcgattg ccatattgca cagggtcata
    13081 cgaccttcca tgcttaaatc acggattgct tcgccgcaaa actccaccac atgcccggtg
    13141 ccgcctgcgc taccagtttt accgataatc gccagcacga tatctttcgc tgtaatgccc
    13201 ggcgcggctt tgccctggac ttcaattttc atggtcttcg cacgaccctg tttcagggtt
    13261 tgcgttgcca gtacgtgttc aacttcggaa gtgccgatac caaaggctag tgcgccaaac
    13321 gcgccgtggg tggcggtatg cgagtcgccg cagacaatgg tcatccccgg caatgtgacg
    13381 ccctgttccg gccccattac gtggacgatc ccctgatacg ggtgattcag gtcatacagc
    13441 tcgacgccaa attctttgca gtttttgatc agctcctgca tctggatgcg cgccatttca
    13501 ccgcaggcat taatgtcttt ggtctgagta gagacgttgt gatccatagt ggcgaaggtt
    13561 ttgcctggct gacgtaccgg gcgaccgtgg gcgcgcagac catcgaacgc ctgcggtgag
    13621 gtcacttcat gcaccaggtg gcggtcgata tataacagcg gggtttcgtt ttcggcttcg
    13681 tacactacgt gagcgtcgaa caatttttcg tataacgttt tagccatgat tacacccctt
    13741 ctaccacata gcgggcaatg atatcgccca tttcattggt actaacggcg gcagcgccac
    13801 gggctaaatc cccggtgcga atgccttctt ctaatgcgcg gttaatggcg cgttcaatgg
    13861 cgcaagccgc atcatctgca tccaggctgt agcgcagcag cagtgccagc gacagaattt
    13921 gcgcaatcgg gttggcgatg tttttgcctg cgatatctgg tgctgagccg cctgccggtt
    13981 catacaaacc aaaaccttgc tcgttcaggc tggcggaagg caacatcccc atcgagccag
    14041 tgatcattgc gcactcgtca gacagaatgt cgccaaacag gttggaacac agcaggacgt
    14101 caaactgtga tggatcttta atcagctgca tggtggcgtt gtcgatgtac atatgcgcca
    14161 gttcgacatc cgggtattcc gtggcgatct cgttgacgat ttcacgccat aaaatagagg
    14221 attgcagcac gttggcttta tcgatcgacg tcactttatg gcgacgttta cgagcagatt
    14281 caaacgcgat gcgggcaata cgctcgatct caaaacggtg atacacctcg gtatcaaacg
    14341 ctttttcata ttgtccgcta ccttcgcggc cttttggctg accgaaatag atgccgccgg
    14401 tcagttcacg cacacacagg atgtcgaagc cgttagcggc aatgtcagca cgcagtggac
    14461 agaatgcttc cagcccctga tacagttttg ccggacgcag gttgctgaat aatttgaagt
    14521 gcttacgcag aggcagcagc gcgccgcgtt ctggttgctg gtctggtggt aaatgttccc
    14581 acttcggacc gcctaccgag ccaaacagca cggcatcggc ttgctcacaa ccttcaaccg
    14641 tcgcaggcgg cagtggttgc ccgtggttat caatggctgc gccgcctaca tcgtaatggc
    14701 tggtggtgat acgcatcgca aatcggttgc gcacggcatc cagcactttc agcgcctgag
    14761 tcatcacttc cggaccaata ccgtcccccg gcaatacggc aatatggtaa ttcttcgaca
    14821 tcacacggtt tccttgttgt tttcgttgtg ttgagctttg cgttgcaact ctttttcgac
    14881 ttctgcggca cgccagatat tgttcagtac gtgcaccatg gctttggcgg aggactcgac
    14941 aatatcggtg gccaggccga cgccgtggaa gcggcgaccg ttgtagttgg cgacaatatc
    15001 cacctgaccc agcgcatctt taccgtgacc tttggcggtc aggctgtatt tcaccagttc
    15061 gacgttatag tcagtgatgc ggtttatcgc ctggtagacg gcatcgaccg gaccgttacc
    15121 gttggcggct tctgctttga cttcttcgcc gcaggccagt ttgacggcgg cagtggcaat
    15181 atcgttagaa cctgactgca cgctgaagta atccagacgg aaatgctccg gctcttcttg
    15241 ctgcttaccg atgaaggcca gcgcctccag atcgtaatca aacacctgac cttttttgtc
    15301 cgccagcttc aggaaggcat cgtacaaatt gtctaaatta tattcacttt ctttataccc
    15361 catctcatcc atgcggtgtt ttactgccgc acgccccgaa cgagaggtca gattcagctg
    15421 gatttggttc aaaccaatag attctggtgt catgatttcg tagttttcgc ggtttttcag
    15481 cacgccatcc tggtggatac cggaggagtg tgcgaatgcg ccgctgccaa caatggcttt
    15541 gtttgccggg atcggcatat tacaaatctg gctaactaac tggctggtgc gccatatctc
    15601 ctggtgatta atagcggtgt ggacgttgag gatatcctta cgaactttga tcgccatgat
    15661 gacttcttcc agcgaacagt taccggcacg ctcgccgatc ccgttcattg caccttccac
    15721 ctggcgtgca ccggcatgta ccgccgccag tgagttgccg accgccaggc ccagatcgtc
    15781 gtgggtatgt acggaaataa tggctttgtc gatgttgggt acgcgttcat acagcccgct
    15841 gatgattccg gcgaactcaa atggcatggt gtagcccacg gtgtccggaa tgttgatggt
    15901 ggtggcaccg gcattaatcg ccgcttcgac cactcgcgcc agatcggcaa ttggggtgcg
    15961 tccggcatct tcgcaagaaa attcaacatc atcggtgtaa ttacgggcgc gtttcaccat
    16021 atagatagcg cgttcgatca cttcgtccag cgtgctgcgc agcttggtgg cgatgtgcat
    16081 tggcgaagtg gcaataaagg tatgaatacg gaaggcttcg gcgactttca gggattcggc
    16141 tgccacgtca atatcttttt ccacgcagcg agctaacgcg catacacggc tatttttaac
    16201 ctggcgagcg atggtctgca ctgattcaaa atcacccggc gaagagacgg ggaaaccgac
    16261 ttccatcacg tcaacaccca tacgctcaag ggccagcgca atttgcagtt tttctttcac
    16321 actcaagctt gcctgtaacg cctgttcacc gtcgcgcaat gtggtatcga aaataatgac
    16381 ttgctggctc atggtttggg tccttgtctc tttttgagcg cctcgcttcg ggcataaaaa
    16441 aacccgcgca atggcgcggg ttttttgttt gactgagtgc tgacttaatt ctgaatgccg
    16501 ctcaccagtc taccgcgcaa agaagatgcg tttagtagta gtagaccgat aaagcgaacg
    16561 atgtgagtca ttaaatcagc tccagatgaa tgcgatatgc ttttagagtt actggatacc
    16621 aaaacggatg tcaaccctga tgcaacaaaa acgtccagcc agcgtgagtt ctgcatccgt
    16681 caaattagct aattgtgctg cggtggttaa aacaagcgat attaatttct gcttaaccac
    16741 cgccgctttt catcggttga catatttcag aataaatttt tgcatctaat cgacgaggga
    16801 aagggggata aaatgcacgc gttacaaaac
//
LOCUS       DAZGQL010000110        16600 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.110, whole genome shotgun sequence.
ACCESSION   DAZGQL010000110 DAZGQL010000000
VERSION     DAZGQL010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16600
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.110"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            225..377
                     /gene="mokC"
                     /locus_tag="ACHKIR_004178"
     CDS             225..377
                     /gene="mokC"
                     /locus_tag="ACHKIR_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HFO9991841.1"
                     /translation="MKQPKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(481..1611)
                     /gene="dnaJ"
                     /locus_tag="ACHKIR_004179"
     CDS             complement(481..1611)
                     /gene="dnaJ"
                     /locus_tag="ACHKIR_004179"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFO9991842.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(1700..3616)
                     /gene="dnaK"
                     /locus_tag="ACHKIR_004180"
     CDS             complement(1700..3616)
                     /gene="dnaK"
                     /locus_tag="ACHKIR_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFO9991843.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEVGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            3992..4396
                     /gene="yaaI"
                     /locus_tag="ACHKIR_004181"
     CDS             3992..4396
                     /gene="yaaI"
                     /locus_tag="ACHKIR_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HFO9991844.1"
                     /translation="MKSVFTLSASLAISLLLCCTAQANDHKILGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQTLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            4422..5135
                     /gene="msyB"
                     /locus_tag="ACHKIR_004182"
     CDS             4422..5135
                     /gene="msyB"
                     /locus_tag="ACHKIR_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFO9991845.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSILGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            5284..5850
                     /gene="satP"
                     /locus_tag="ACHKIR_004183"
     CDS             5284..5850
                     /gene="satP"
                     /locus_tag="ACHKIR_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HFO9991846.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(5885..6472)
                     /gene="mog"
                     /locus_tag="ACHKIR_004184"
     CDS             complement(5885..6472)
                     /gene="mog"
                     /locus_tag="ACHKIR_004184"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HFO9991847.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAAGNVVVHGIFA
                     SVPYCIQLLEGPYIETAPEVVAAFRPKSARRDVSE"
     gene            complement(6587..7540)
                     /gene="tal"
                     /locus_tag="ACHKIR_004185"
     CDS             complement(6587..7540)
                     /gene="tal"
                     /locus_tag="ACHKIR_004185"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFO9991848.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAVDQEKLEKMIGDLL"
     gene            7819..9249
                     /gene="yaaJ"
                     /locus_tag="ACHKIR_004186"
     CDS             7819..9249
                     /gene="yaaJ"
                     /locus_tag="ACHKIR_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFO9991849.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITTGGPGAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDVNGQFRGGPAWYMARGLGMRWMGVLFALFLLIAYGIIFSG
                     VQANAVARALSFSFDFPPLVTGIILAVFALLAITRGLHGVARLMQGFVPLMAIIWVLT
                     SLVICVMNIGQLPHVIWSIFESAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMG
                     STPNAAAAAASWPPHPAAQGIVQMIGIFIDTLVICTASAMLILLAGNGTTYMPLEGIQ
                     LIQKAMRVLMGSWGAEFVTLVVILFAFSSIVANYIYAENNLFFLRLNNPKAIWCLRIC
                     TFATVIGGTLLSLPLMWQLADIIMACMAITNLTAILLLSPVVHTIASDYLRQRKLGVR
                     PVFDPLRYPDIGRQLSPDAWDDVSQE"
     gene            9319..10095
                     /gene="yaaA"
                     /locus_tag="ACHKIR_004187"
     CDS             9319..10095
                     /gene="yaaA"
                     /locus_tag="ACHKIR_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HFO9991850.1"
                     /translation="MLILISPAKTLDYQSPLPTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(10135..10431)
                     /locus_tag="ACHKIR_004188"
     CDS             complement(10135..10431)
                     /locus_tag="ACHKIR_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HFO9991851.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEMTLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPYGPPPSPRHNKHNDHRGDHRPGPDKHHR"
     gene            complement(10645..11931)
                     /gene="thrC"
                     /locus_tag="ACHKIR_004189"
     CDS             complement(10645..11931)
                     /gene="thrC"
                     /locus_tag="ACHKIR_004189"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HFO9991852.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFVFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLHPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(11932..12864)
                     /gene="thrB"
                     /locus_tag="ACHKIR_004190"
     CDS             complement(11932..12864)
                     /gene="thrB"
                     /locus_tag="ACHKIR_004190"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HFO9991853.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(12866..15328)
                     /gene="thrA"
                     /locus_tag="ACHKIR_004191"
     CDS             complement(12866..15328)
                     /gene="thrA"
                     /locus_tag="ACHKIR_004191"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HFO9991854.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHLLRHAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(15354..15473)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(15409..15474)
                     /gene="thrL"
                     /locus_tag="ACHKIR_004192"
     CDS             complement(15409..15474)
                     /gene="thrL"
                     /locus_tag="ACHKIR_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HFO9991855.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(15688..16374)
                     /gene="yjtD"
                     /locus_tag="ACHKIR_004193"
     CDS             complement(15688..16374)
                     /gene="yjtD"
                     /locus_tag="ACHKIR_004193"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFO9991856.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVLLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADKHQLQALRERAMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
BASE COUNT         3865 a         4429 c         4093 g         4213 t
ORIGIN      
        1 ataaaaatgc gaggcgtctt caggattaga atcgataaaa cagaatgggt taaagagagc
       61 gatataatag cggcgggtgc ttgaggctgt ctgtctcagg cattagctga acggcagata
      121 gagaaaagcc ccgagtgata ttttactatc aacccgaggc cccctatatg cagaacacat
      181 gtagagtgcc tcttactgac cgtaaggtca aggagaagag agcaatgaag cagcctaagg
      241 cgatgattgt cgccctgatc gtcatctgta tcaccgccgt agtggcggcg ctggtaacga
      301 gaaaagacct ctgtgaggtt cacatccgaa ctggccagac ggaggttgct gttttcacgg
      361 cttacgaatc cgagtaagag caacggcggg gagtgatccc cgccactttt caggtgctcg
      421 catatcttca acgcacccta tttttaccca ggcctgccca cgggcaggct tttggggaga
      481 ttagcgggtc aggtcgtcaa aaaacttctt cacaccatca aagaagctct ttgagcgcgg
      541 gctgttgtgc tcgccggttg ggccaccgaa gctttcttgc agctcttgca gcagctgttt
      601 ctgcttctcg ttcaggccta ccggtgtttc gacgacaacg cggcacagca aatcaccctg
      661 tgcgccaccg cggacagact tgacgccttt accgcgcata cggaacagct taccggtctg
      721 ggtttcgcca ggcactttca gtttgacgcg accatcaagg gtcggtactt cgatttcgcc
      781 acccagcgcc gccatagcga agttgatcgg gacttcgcaa tagaggttgt tgccttcacg
      841 ctcgaaaatc gggtgctgtt taacctgaac ctgaacgtac agatcgcctg ccggtgcgcc
      901 atgctcgccc gcttcacctt cgcccgcaag acggatgcgg tctccagtgt ccacccctgc
      961 cgggatttta acggacagcg ttttgctgcg ctcaacacga ccatgaccat gacatttgtt
     1021 gcacggatct ttgatcagcg taccgcggcc ctgacagtgt ggacaggtct gctgcacggc
     1081 aaagaaaccc tggcgcatct gcacctgacc agaaccatga caggtcggac aggtctgcgg
     1141 ctgtgtacct ggttttgcac cgctaccgtg gcaaacgtca cactcttcca gagtcggaat
     1201 gcggatctct ttggtcacgc cacgtacagc ttcttcgagg gtgagctcca tgttatagcg
     1261 taaatcagca ccgcgcgccg cacgttgacg accacgtccg ccgccaaaaa tatcgccgaa
     1321 aacgtcacca aaaatatcgc tgaagtctgc gccgccgccg aaaccgccgc ctcccatgcc
     1381 accttgctca aacgcagcat gaccatactg atcgtatgcc gcacgttttt gcgagtcggt
     1441 cagaacttca taagcttcct tgatctcttt aaatttcgcc tcggcctctt tgtcaccctg
     1501 gttacggtcc gggtggtatt tcatggccag gcgtttgtag gcctttttga tttcacgctc
     1561 ttccgctgtt ttggaaacgc ctaaaatctc gtaataatct tgcttagcca tcttttttaa
     1621 attgccccta gatgaatgca cgggcggaga ggaaattccc cttcgcccgt gtcagtataa
     1681 ttacccgttt atagggcgat tattttttgt ctttgacttc ttcaaattca gcgtcgacaa
     1741 catcgtcatc tttcgcgttg tttgcagaag catcagcacc ggcagtctgc tgctgggcat
     1801 gttgctgctg ggcgatttcc atcagtttct gggaaacctg tgccagttcc tgcattttcg
     1861 cttcgatagc ggctttgtct tcacctttca gagcagtttc cagtgcagtc agtgcagact
     1921 cgatagcagt tttgtcgtca gccggcagtt tgtcgcccac ttcttcaacc tgcttacggg
     1981 tgctgtgcag cagatggtcg ccctggttgc gagtctgtac cagctcttca aacttacggt
     2041 cagcttcggc gttagcttct gcgtcgcgta ccattttctg gatttcatct tcgttcagac
     2101 cagaagaagc cttgatggtg atcttctgct ctttaccgct gtttttatct ttcgcggaaa
     2161 cgtgcaggat accgtcagca tcgatatcga aggtaacttc gatctgcggc atgccgcgcg
     2221 gtgccgggtt gataccatcc aggttgaact gacccagaga tttgttatca gctgcacgtt
     2281 tacgttcacc ctgcagcaca tggatggtta ccgcagactg gttgtcttca gcggtagaga
     2341 acacctggct gtgcttggtc gggatagtgg tgtttttcgc gatcagcgtc gtcatcacac
     2401 cgcccatggt ttcgataccc agagacagcg gggtaacgtc cagcagcagt acgtctttta
     2461 cgtcaccagt cagaacacca ccctgaacag cagcaccgat tgctacagct tcgtccgggt
     2521 taacgtcttt acgcggctct ttaccaaaga actcagcaac tttcttctga accattggca
     2581 tacgagtctg accaccaacg aggataacgt cgtcgatatc agatacggac aggccagcgt
     2641 cctgcagtgc aactttcagc ggctcaatgg aacggtttac cagatcttca accaggcttt
     2701 ccagtttcgc acgagtcact ttgatgttca tgtgtttcgg accggtcgcg tctgcagtga
     2761 tgtacggcag gttaacgtcg gtctgctgag cggaagacag ttcgattttc gctttttctg
     2821 ccgcttcttt caggcgctgc attgccagcg gatcgttgcg caggtcaatg ccctgatctt
     2881 tcttgaattc ttcaaccagg tagttgatca gacggctgtc gaagtcttca ccacccaggt
     2941 gggtatcacc gttggttgcc agaacttcga aggttttttc gccgtcaact tcgtcgattt
     3001 cgataataga aatatcgaaa gtaccaccac ccaggtcata aaccgcgata gtacggttgc
     3061 cagtaccttt gtccagaccg taagccagcg cagctgcggt cggttcgttg atgatacgtt
     3121 ttacttccag accagcgata cggcctgcgt ctttggttgc ctgacgctga gcatcgttaa
     3181 agtatgccgg tacggtgata acagcttcag ttaccggttc acccaggtaa tcttcagcgg
     3241 ttttcttcat ttttttcagc acttcagcag agatctgcgg cggtgccatt ttctggcctt
     3301 taacttcgac ccatgcgtcg ccgttatcag cagcaataat tttgaacggc atgatggaaa
     3361 catcacgctg tacttcttcg tcctggaagc ggcggccaat caggcgttta atcgcaaaca
     3421 gggtgttttg cgggttcgtc actgcctgac gtttagccgg ctgaccaacc agagtttcac
     3481 catcctgggt ataggcaatg atagaaggcg tggtgcgatc gccttcggcg ttctccagta
     3541 cacgaggagt ggtgccatcc ataatcgcta cacaagagtt ggtagtaccc aggtcgatac
     3601 caattatttt acccatctaa acgtctccac tatatattcg gtcatcatgt ggttgtgagt
     3661 ctgtaatagg ggcgaaacgt ctggtttcaa ctgcccaatt tcattttttt gcagactcac
     3721 tcactgcggt tgactactaa atggggtcgt aacccacgtc atcaaggggg agatgcaaaa
     3781 attttttgtg cttttgcgca gaaatttcgc gatatgccag aacaagacat gaaactgaca
     3841 gcaattttat tcatatagag aatgaatagc agacaagaca ttcatagaaa aattgtaata
     3901 aaaagaaaat attacgtacc tgaatcttct ctttatcagc agtaaactag tgggtattca
     3961 tcccctacct ctccccacta agagaatcct tatgaaatcc gtttttacgc tttccgccag
     4021 cctggcgatt agcctgctgc tgtgctgcac ggcgcaggca aacgaccata aaatcctcgg
     4081 cgtcattgca atgccacgta acgaaaccaa cgatctggcg ctgaaacttc ctgtttgccg
     4141 aattgttaaa cgcatacaac tctcagccga ccatggcgat ttacagttaa gcggtgcatc
     4201 ggtttatttc aaagccgccc gtagcgccag tcagaccctg aatattcctt cagaaataaa
     4261 agaagggcaa accactgact ggatcaacat taacagcgat aacgacaata aacgctgcgt
     4321 ctcaaaaatc accttttctg gtcatacggt gaactcatcg gatatggcca cgctgaaaat
     4381 tatcggcgac gactaacgcc agaatataga agccacaaaa aatgaatgtt aattacctga
     4441 atgattcaga tctggatttt ctccagcatt gtagtgagga acaactggca aatttcgccc
     4501 ggctgctcac ccataatgaa aaaggcaaaa ctcgcctctc cagcgtactg atgcgcaacg
     4561 aactgtttaa atcgatggaa ggacatcccg agcaacaccg ccgcaactgg cagctgattg
     4621 ccggagaatt acaacatttt ggtggcgata gtatcgccaa caaactgcgc ggacacggta
     4681 aattgtatcg ggccattttg ctcgatgttt caaaacgatt gaagctgaaa gccgacaaag
     4741 agatgtctac gtttgaaatt gagcaacagt tactggaaca atttctgcgt aatacctgga
     4801 agaaaatgga cgaggaacat aagcaggagt ttctgcacgc ggtcgatgcc agggtgaatg
     4861 agctggaaga gctgctgccg ctgctgatga aagacaaatt attggcaaaa ggcgtgtcgc
     4921 atttgctttc cagccaattg acccgcattt tacgcaccca cgcagcaatg agcatacttg
     4981 ggcatggttt gcttcgcggc gcggggctgg gaggccctgt aggcgcggca ctaaatgggg
     5041 ttaaagcggt cagcggcagt gcctatcgcg tgacaattcc agccgtactg caaatcgcct
     5101 gcctgcgccg gatggttagt gccactcagg tctgataccg gaaaccgggg aaaattttca
     5161 ttttcccctc aaaagatcgt agacactgcc ccactggctg attattatgc cgcgccctga
     5221 aaacactaca gttattcagg gaaattattt caccattcat tcgatgatga tttttgagga
     5281 attatgggca acactaagtt ggctaatccg gcaccgctgg gcctgatggg cttcggcatg
     5341 accaccattc tgcttaacct gcacaacgtg ggttattttg ctctggacgg tattattctt
     5401 gccatgggca ttttctacgg cggcatcgcg caaatttttg ctggtctgct ggagtacaaa
     5461 aaaggcaaca ctttcggttt aaccgcattc acctcttacg gttctttctg gctgacgctg
     5521 gttgcgattc tgctgatgcc gaaactgggt ctgaccgatg cgccaaatgc acagttcctt
     5581 ggtgtctacc tgggtctgtg gggcgtattt acgctgttta tgttcttcgg cacgctgaaa
     5641 ggcgcacgcg ttctgcaatt cgttttcttt agcctgaccg tgctgtttgc cctgctggcg
     5701 atcggtaaca ttgccggtaa cgccgcaatc atccactttg ccggctggat tggtctgatc
     5761 tgcggtgcca gcgcaatcta tctggcgatg ggtgaagtac tgaacgagca gtttggtcgc
     5821 accgttctgc cgattggtga atcccattaa ttgttttgag atccccccgc tcggggggat
     5881 ttttttattc gctaacgtcg cgtcttgcac tcttcggtct gaatgctgca accacttccg
     5941 gtgccgtttc aatgtatggc ccttccagca actgaatgca gtacggtacg ctggcaaaaa
     6001 taccatgcac cacaacgtta cccgcagcgt ccttcacgcc ttccagcgtc tctttaatcg
     6061 acttcggctg accgggtaag ttaaggatca gcgcctgttt gcgaatcacg cccacctgac
     6121 gcgaaaggat cgcagttggt acaaaatgca ggctgatctg gcgcatctgt tcaccaaagc
     6181 caggcatctc gcggtccgct actgccagcg tcgcatcggg cgttacgtca cgacgcgccg
     6241 ggccagttcc gcccgtggtg agcaccagat ggcaactcat ttcatccacc agctcacaca
     6301 acgtttgctc gatgatcgcc tgctcatcgg ggattaagcg ggtttccagt tcaaacggcg
     6361 tggttagcgc cgatgtcagc cattcttcca gcgcagggat gcctttatcc tgataaacgc
     6421 cgctggatgc gcgatcagag atggaaacta agccaatacg taaagtattc atcaagattt
     6481 ttccgtttaa acagtctcgt taaacagaat gatacactgc gaagggagtg acagacaggc
     6541 ttcagaaaag gtagcgtgac cgacttcccg gtcacgctaa gaatgattac agcagatcgc
     6601 cgatcatttt ttccagtttt tcctggtcaa cagcaaactt acggatacct tccgccagtt
     6661 tatctactgc cattggatcc tggttatgct gccacaggaa ctcggactca gtgatacgcg
     6721 ccggacgcgc tttcacttcg ccggtgtaag acagtttacg ttcgatagcc ccttcgctct
     6781 cagccagctc tttcagcagc gccggtgcga tggtcagacg gtcgcagcct gccagttcca
     6841 gaatttcgcc gatgttacgg aagcttgcgc ccatcaccac ggtttcataa ccgtgctctt
     6901 tgtagtactg gtagatttca gatacagaaa ccacgcccgg atcttctgcc ggagcgtact
     6961 ctttcttgtc ggtattcgct ttgtaccagt caagaatacg gccaacaaac ggcgagatca
     7021 ggaacacgcc cgcttccgca caagcacgag cctgagcgaa ggagaacagc agggtcaggt
     7081 tacagttaat accttctttt tccagctgtt ctgcagcacg gataccctgc caggtagaag
     7141 ccagtttgat cagaatacga tcgttgctaa tccctgcatc gttgtagagt ttgatcaggc
     7201 gttttgcttt cgcaattgac gcttcggtgt cataggaaag acgcgcatca acttcagttg
     7261 agatacggcc cggaaccagt ttcaggattt ccagaccaat atttactgcc agtttgtcgg
     7321 tcgcgtccac gatctgctgc gcgcgatcgt tgctctgctg tttcgcccag gcgacagcat
     7381 catcaatcaa cttacggtat tccggaatct gcgctgcgtt aagaatgaga gaagggttgg
     7441 ttgtggcatc ctgcggttga tacagcttca ttgccgcgat gtccccagtg tcggccacta
     7501 cggtggtgta ctgacgaagg gaggtcaatt tgtccgtcat gatagtattt ctctttaaac
     7561 agcttgttag ggggatgtaa ccggtctgcc ctgatgatat cacgacgcaa cgtcagcgca
     7621 accgccgaca agacggttat cgccaggcga gactgtttcg gatttctgac actgcgttga
     7681 tatcgcccgc catttttata caaaacctca tgtatgctac gcagaagtta tcaagtacct
     7741 cgtagcgtat atacttctta aacaattggt aacgtttaca caggaaagtc atcgcgaccg
     7801 gcaataagag ggatatgcat gccagatttt ttctccttta ttaacagcgt cctttgggga
     7861 tcggtaatga tttacctgct cttcggcgca ggttgttggt tcacttttcg caccggattt
     7921 gtgcagtttc gctacatccg ccagtttggc aaaagtctta aaaatagcat tcatccacag
     7981 ccaggcggtt taacctcatt tcagtcattg tgtaccagtc ttgcggcgcg cgtgggtagc
     8041 ggcaacctgg ctggcgttgc gctggctatt accaccggtg gaccaggtgc cgtcttctgg
     8101 atgtgggttg ccgcgtttat cggcatggcg acctcgtttg ccgaatgttc ccttgcacaa
     8161 ctttataaag aacgtgacgt taatgggcag tttcgtggcg gaccggcatg gtatatggcg
     8221 cgcgggctgg ggatgcgctg gatgggcgtt ctgttcgccc tctttttgct catcgcctac
     8281 ggcataattt tcagcggagt tcaggcgaac gccgttgccc gagccctgag tttttctttt
     8341 gattttcccc cgctggtgac aggcattatt cttgctgtct ttgctctgct ggcaatcact
     8401 cgcggtcttc atggcgtcgc ccggctcatg caggggtttg tcccgttgat ggcgataatc
     8461 tgggtactga ccagcctggt aatatgcgta atgaatatcg ggcaacttcc ccacgtcatt
     8521 tggtctattt ttgagagtgc ttttggctgg caggaagcgg caggcggcgc ggcgggatat
     8581 accttaagcc aggcgattac taacggtttt cagcgcagta tgttttccaa tgaggcggga
     8641 atggggtcga cgccaaacgc ggcagcggcg gctgcgtcct ggcctccgca tcccgcagcg
     8701 caaggaattg tccagatgat tggcattttt atcgacaccc tggtcatctg tacggcaagc
     8761 gccatgctga tattactggc gggtaacggc acaacctaca tgccgctgga aggtattcag
     8821 cttatccaga aggcgatgcg ggtactcatg ggttcctggg gtgctgagtt tgttaccctc
     8881 gtggttattc tgtttgcctt cagctccatc gttgccaact acatttatgc cgaaaacaat
     8941 ctcttcttct tacgcctgaa caaccctaaa gcgatctggt gtctgcggat ctgcaccttc
     9001 gcaacggtca tcggcggcac cttgctaagt cttccgctga tgtggcaact ggcagatatc
     9061 ataatggcct gcatggctat taccaatttg accgccatat tactgctctc gcctgtggtt
     9121 cataccattg ccagtgatta tctacgccag cgtaaactcg gcgtgcgccc ggtgtttgat
     9181 ccgttgcgtt atccggatat cggtcgccag ctttctccgg acgcgtggga tgatgtttcg
     9241 caggagtaat cacaactatc gatcaactca ttctcttttt ttgctaaagt cggcataaat
     9301 ttcctgcaag gactggatat gctgattctt atttcacctg cgaaaacgct cgattaccaa
     9361 agcccgttgc ccaccacgcg ctatacgctg ccggagctgt tagacaattc ccagcagttg
     9421 atccatgaag cgcggaaact gacgcctccg cagattagca cgctgatgcg catcagcgac
     9481 aaactggcgg gtatcaacgc cgctcgcttt catgactggc agccagattt cacgccggag
     9541 aatgcccgcc aggcgattct ggcattcaaa ggtgatgtct acaccggctt gcaggccgaa
     9601 accttcagcg aagacgattt cgattttgcc caacagcatt tgcgaatgct ttccggcttg
     9661 tatggcgtac tgcgcccgct cgatttaatg cagccttatc gtctggaaat ggggatccgt
     9721 cttgagaatg cccgagggaa agatctgtat caattctggg gagatatcat caccaacaag
     9781 ctgaacgagg cgctcgcagc acaaggcgat aatgtggtga ttaatctggc gtcagatgag
     9841 tattttaaat cggtgaagcc gaagaaattg aatgccgaga ttatcaagcc ggtgttcctc
     9901 gatgagaaga acggcaaatt taagatcatc agcttctacg ctaagaaagc acgcggtctg
     9961 atgagtcgtt tcattattga aaatcggctg accaaaccag agcaactgac tggttttaat
    10021 agcgaaggtt acttctttga tgaagcttcc tccagcaacg gcgaactggt gtttaaacgc
    10081 tacgagcagc gttaagtttg cagtcaatgc cggatggcaa tccggcattg atatttagcg
    10141 atgatgtttg tcaggccccg gacgatgatc gccacgatga tcattgtgct tgttatggcg
    10201 cggcgatggc ggcggtccat atgggtgcca gcgattgcct cgccattcat aatgttgttt
    10261 ccaccagccg tggtcgcgcc agtgaccgcc atcccagtaa tagccacgat tatcacgatc
    10321 gcctatctgt aattttactg acgggactaa cgtcatttcc gcagcctgcg cagccatggg
    10381 agcgaccaga accagggaaa gtgcgagtac gatagattgc atctttttca caggttactc
    10441 cttttatcat tgcccgttat gggtcgatat ccggagatta cgcgatgaga acggcagttg
    10501 ttattctccg caatcctaat ctctaagtta ccgcatttat tgagcatttc tcctttttcc
    10561 ctgtcgtttt tctccataat ttcttcataa aaaagccggg ctgcataaaa gcaaacccgg
    10621 cctgattgag ataatgaata gatattactg atgattcatc atcaatttac gcaacgcagc
    10681 aaaatcggcg ggcagattat gtgaaagcaa gggtaaatcg gcacgttctg ccagctcttt
    10741 tggcagatcc aacgtttcac cgagaatcgc ttccacgctc tctttaaatt tcgccggatg
    10801 cgcggtgccg aggaacaagc catattcgcc tggatgcaac tggtcacgca gtgcacgata
    10861 cgctacggca gcgtgcggct ccgaagtgta gcccagttct tttaactcac gcattgtctg
    10921 ttgcgtggtt tcatcatcca cggctgcata acccagctct ttcagttgcc agattttgcg
    10981 gcggaacaac tcttccacac gcggccagtt gttcggctga ctcacgtcca tcgcgttgga
    11041 taacgtcgcc tgagtcgctt tgggtgacca ctgaccgtcg tgcaggaaac gtggcacggt
    11101 atcgttcacg ttggtcgcag caataaaacg tttcaccggc agaccgagtg acttcgccag
    11161 cagacccgcc gtcaaatcgc cgaagtttcc gcttggcacc gagacaacca gctggttgcg
    11221 cgcctcctgc ggcagctgcg caacagcttc aaagtagtag caaatctgcg ccagcaaacg
    11281 gctgatgtta atcgagttag ctgagtttag ccccagcgcc actttcagtt cttcatcatc
    11341 aaacgcctgc ttcaccagcg cctgacaggc atcgaaatcg ccgtcgatgg caacagtttc
    11401 aatattgccg cccaatgtac agaacagttt ttcttgcagt ggactgattt tgcctcgtgg
    11461 atagaggata accactttca cattcggcaa accgtagaaa gcatgagcca ctgccgctcc
    11521 ggtatcaccg gaggtcgcgg tcagaatggt cactggctta tcgcccgcaa tatgggtcag
    11581 catttgtgcc ataaagcgac cgccgaaatc tttaaatgcc agcgttggcc cgtggaacaa
    11641 ttccagacaa ccgacatcgc tttcaacatt ggcgaccgga gccgggaaga caaacgccgc
    11701 gcgtacgcgc tcttccagga tttcctgcgg gatttcatca ccaataaacg ccgagaggat
    11761 cttcgcactg cgggtgacaa aatccagctt cagcatctca tcaatttcag tcaggctgaa
    11821 ttccggcagg tcgtgcggga aaaacagccc ctgatttttg cccaacccct gggttacggc
    11881 ttgcgcaaag ctgacctgct cattgtgatc tttcagattg tagagtttca tttagttttc
    11941 cagtactcgt gcgcccgccg tatccagccg gcaaatatga acaaaacctt cctgattttg
    12001 caggtagttc ttacccaacc agtcggcaac gcgctgggcg gtatccggct tgtcacacag
    12061 agcgaacaaa gtcgggccgg agccggagat accgctcgct accgcgccga tttccgcgac
    12121 cgcctgccgc gcctgccgga agcctggcag taaccgttca cggtagggtt cagcgataac
    12181 atctttcatc agcttcgcgg caagctcagg ctgacgggaa tagcaggcgt gaataaagcc
    12241 tgccagatgt cgcccgtgcg caatgcaatc ctggcggcga tactgcgccg gtaaaatagc
    12301 cctggcttct gccgtcgaga ctttaatccc cggatacgcc agcacccaca gccactcatc
    12361 aaaccctggc acttgctggc tgatgatgtc gttttcttcg atcatcaact gcataccacc
    12421 gagaaaacac ggtgccacgt tgtcgtaatg aatgctgccg gagatacgcc cttccagctc
    12481 gcccatcaaa gccagcaaac gggtgtcatt aagcggcttg ccgcagtgtt cattcatcgc
    12541 catcagcgcc gcgaccactg agcaggcgct ggagcctaag cccgagccga tcggcatatt
    12601 cttttccagg gtcatcgcca ctggaatttg cttacccagc tcctggcaaa aacgctccca
    12661 acactgataa acgatatttt cccgaggttc tgacggcagc ttatcggcaa agcgtccgag
    12721 gttattgaga ctgaatgtct ctgccgcctc aaccgtgact acatctccga gcaatgcacc
    12781 atcaacgggt gtcaccgccg ccccgagcac atcgaacccg acgctcatat tggcactgga
    12841 agccggggca taaactttaa ccatgtcaga ctcctaactt ccatgagagg gtacgtagca
    12901 gatcggcaaa gacaccggca gctgtaacgt cattgcccgc accatatccg cgcagcacca
    12961 acggcagcgg ctgataatag tggctataga aggccagggc gttttcgccg tttttcactt
    13021 tgaacagcgg atcgttgcca tccacttcag caatcttcac gcggcagacg ccatcttcat
    13081 caatattgcc aacatagcgc aaaacttttc cttcatcacg ggccttcgcc acacgagcgg
    13141 caaagagatc atcgagctgt gacagattcg ccataaaagc ggcaacatca ccctcggcgt
    13201 taaactcagc gggtagcaca ggttcaattt caatatccgc cagctccagt tcacgtcccg
    13261 tttcacgagc gagaatcaat agtttacgcg ccacatccat accagaaaga tcatctcgcg
    13321 gatccggttc ggtataaccc atttcccgcg ccaacgtggt cgcctcggag aaactcatgc
    13381 cttcgtctaa cttgccgaag atataagaaa gcgaaccaga aagaatgccg gagaacttca
    13441 tcaattcatc acctgcattg agcagatttt gcaggttctc aataaccggt aatccagccc
    13501 caacgttggt gtcatagagg aatttacgcc gcgatttttc cgccgcatga cgcaacagat
    13561 ggtagtaatc catcgacgag gtgttagcct ttttgttcgg cgtgacgacg tggaaacctt
    13621 cgcgcaggaa gtcggcatat tgatccgcca ctgcctggct ggaggtgcag tcaacaatga
    13681 ccgggttcag cagatgatat tctttcacga ggcgaattaa gcgcccgaga ttaaacggct
    13741 ctttggcttg cgccagttct tcctgccagt tttccagatt aaggccatgt acattggtga
    13801 gcagagcctt cgagttggca acgccgcaga cacgtaagtc gatatgttta ttcttcaacc
    13861 agctttgctg acgcttcagt tgctccagca gcgcaccgcc aacgccaccg acgccaatca
    13921 caaacacttc gataacctga tcggtattga acagcatctg atgagtaacg cgcacgccag
    13981 tggtcgcatc atcgttattt accacgacag agattgagcg ttcagaagat ccctgagcaa
    14041 tggcgacaat gttgatattg gcgcgggcca gcgcggcaaa gaatttcgcc gagatcccac
    14101 gcaaggtgcg cataccatca cctaccaccg agataatggc cagccgttcc gtcaccgcca
    14161 gcggctccag taagccttct ttcagttcca ggtagaactc ttcctgcatt gcccgttcag
    14221 ctcgcacaca gtcgctttgt ggaacgcaga aactgatact gtattcggaa gatgattgcg
    14281 taatcagcac cacggaaata cgggcgcgtg acatcgctgc aaagacgcgc gccgccatgc
    14341 cgaccatccc tttcatcccc ggaccagaaa cgctgaacat tgccatgtta ttcagattgg
    14401 aaatgccctt gaccggtaat tcgtcttcat cacggctggc accaatgagc gtacctggtg
    14461 cttgaggatt tccggtattt ttaatcaggc aagggatctg gaactgggcg atgggggtaa
    14521 ttgtgcgggg gtgaagaact ttagcgccga agtaggaaag ctccatcgct tcctggtagg
    14581 acatcgactt caacaacctc gcatcgggca cctgacgcgg gtcgcaggta tagaccccgt
    14641 caacgtccgt ccaaatctcg caacaatcgg cgcgtaaaca ggcagccagc accgccgcgg
    14701 agtagtcgga accgttacgt ccaagcacca ccagttcgcc tttttcatta ccggcggtga
    14761 aacctgccat caacaccatg tgatcagccg gaatacgact tgccgcaata cggcgggtgg
    14821 actcagcaat atcgacggta gattcgaggt aatgccccac tgccagcagt ttttcgaccg
    14881 gatcgataac ggtaacgttg tgaccacgcg cttctaacac gccggccata atggcgatcg
    14941 acattttctc gccacggcaa atcagcgcag cgttgatgct atccgggcac tgccccaaca
    15001 aactaatgcc atgcaggaca tgttttattt gggcaaattc ctggtcgacg aaagttttca
    15061 gttgcgccag cgggaatccc ggctgagcgg cggcgagtcc cgtcagaagt tcggcaaaaa
    15121 tacgttcggc atcgctgata ttgggtaaag catcctggcc gctaatggtt ttttcaatca
    15181 tcgccaccag gtggttggtg attttggcgg gggcagagag gacggtggcc acctgcccct
    15241 gcctggcatt gctttccaga atatcggcaa cccgcagaaa acgttctgca tttgccactg
    15301 atgtaccgcc gaacttcaac actcgcatgg ttgttacctc gttacctttg gtcgaaaaaa
    15361 aagcccgcac tgtcaggtgc gggctttttt ctgtgtttcc tgtacgcgtc agcccgcacc
    15421 gttacctgtg gtaatggtga tggtggtggt aatggtggtg ctaatgcgtt tcatggatgt
    15481 tgtgtactct gtaattttta tctgtctgtg cgctatgcct atattggtta aagtatttag
    15541 tgacctaagt caataaaatt ttaatttact cacggcaggt aaccagttca gaagctgcta
    15601 tcagacactc tttttttaat ccacaccgag acatattgcc cgttgcagtc agaatgaaaa
    15661 gctgaaaaat acttactaag gcgtttttta tttggtgata tttttttcaa tatcatgcag
    15721 caaacggtgc aacattgccg tgtctcgttg ctctaaaagc cccaggcgtt gttgtaacca
    15781 gtcgaccagt tttatgtcat ccgccactgc cagagtcgtc agcagtgcca tggctcgttc
    15841 gcgtaaagct tgcagttgat gtttgtctgc cgttgtgtca cttttcgccg gttgttgtat
    15901 taatgttgct aattgatagc aatagaccat caccgcctgc cccagattga gcgaaggata
    15961 atccgccacc atcggcacac cagtaagaac gtcagccaac gctaactctt cgttagtcaa
    16021 cccggaatct tcgcgaccaa acaccagcgc ggcatggctc atccatgaag atttttcctc
    16081 taacagcagc accagttcta ctggcgtagc gtagtaatga tatttcgccc gactgcgcgc
    16141 agtggtggca acagtgaaat cgacatcgtg taacgattca gccagtgtcg ggaaaacttt
    16201 aatattatca ataatatcac cagatccatg tgcgacccag cgggtggctg gctccaggtg
    16261 tgcctgacta tcgacaatcc gcagctcgct aaaccccatc gttttcattg cccgcgccgc
    16321 tgccccaata ttttctgctc tggcgggtgc gaccagaata atcgttatac gcatattgcc
    16381 actcttcttg ctcaaataac cgcgaaccgg gtgaccactg tcaacttatt acgcggtgcg
    16441 aatttacaaa ttcttaacgt aagtcgcaga aaaagccctt tacttagctt aaaaaaagct
    16501 aaactatttc ctgactgtac taacggttga attgttaaaa aatgctacat atccttatgt
    16561 ttacttagga taattttata aaaaataaat ctcgacaatt
//
LOCUS       DAZGQL010000111        16544 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.111, whole genome shotgun sequence.
ACCESSION   DAZGQL010000111 DAZGQL010000000
VERSION     DAZGQL010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16544
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.111"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..351
                     /locus_tag="ACHKIR_004194"
     CDS             <1..351
                     /locus_tag="ACHKIR_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFO9991857.1"
                     /translation="AYLADEIAKLGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTLYD
                     LSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSDHP
                     KLQGIAQQNSFKHT"
     gene            719..1543
                     /gene="gadX"
                     /locus_tag="ACHKIR_004195"
     CDS             719..1543
                     /gene="gadX"
                     /locus_tag="ACHKIR_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HFO9991858.1"
                     /translation="MQPLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIQV
                     DKCVENFVLVSRDTLSLFLPMLKEEALKLHVHKKVPSLLVHHCTRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDTQFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEGTSYSQLLTECRMQRALQLIVIYGVSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQELPNCGSAASMAAQGIFYGTDRSAEGIRL"
     gene            1912..2640
                     /gene="gadW"
                     /locus_tag="ACHKIR_004196"
     CDS             1912..2640
                     /gene="gadW"
                     /locus_tag="ACHKIR_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HFO9991859.1"
                     /translation="MAHVCSVILVRRSFDIHHEQQKISLHNESILLLDKNLAADFAFC
                     SLDTRRLDIEELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPIQLVTAIFDSFNDPR
                     VNSPILSKMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFSQHSPGTFS"
     gene            complement(3003..6116)
                     /gene="mdtF"
                     /locus_tag="ACHKIR_004197"
     CDS             complement(3003..6116)
                     /gene="mdtF"
                     /locus_tag="ACHKIR_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
                     /protein_id="HFO9991860.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIK
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGDHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKNNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPNQVIGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPVEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(6141..7250)
                     /gene="mdtE"
                     /locus_tag="ACHKIR_004198"
     CDS             complement(6141..7250)
                     /gene="mdtE"
                     /locus_tag="ACHKIR_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015870489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HFO9991861.1"
                     /translation="MLTACDDKSAENAAAMTPEVGVVTLSPGSVNVLSELPGRTVPYE
                     VAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELNSAKGSLAKALSTASNA
                     RITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAVEQATINLQYANVTSPI
                     TGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQDFLRMKEEVASGQIKQV
                     QGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIFPNPNGDLLPGMYVTAL
                     VDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIEASKAIGDQWVVTSGLQ
                     AGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            7358..7636
                     /locus_tag="ACHKIR_004199"
     CDS             7358..7636
                     /locus_tag="ACHKIR_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9991862.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYIYQGNKVVFASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(7637..8164)
                     /gene="gadE"
                     /locus_tag="ACHKIR_004200"
     CDS             complement(7637..8164)
                     /gene="gadE"
                     /locus_tag="ACHKIR_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HFO9991863.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNNPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(8963..9535)
                     /gene="hdeD"
                     /locus_tag="ACHKIR_004201"
     CDS             complement(8963..9535)
                     /gene="hdeD"
                     /locus_tag="ACHKIR_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HFO9991864.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSATSLFSFASLFVKKQ"
     gene            9790..10122
                     /gene="hdeA"
                     /locus_tag="ACHKIR_004202"
     CDS             9790..10122
                     /gene="hdeA"
                     /locus_tag="ACHKIR_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HFO9991865.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKKASFKDKVKGE
                     WDKIKKDM"
     gene            10238..10564
                     /gene="hdeB"
                     /locus_tag="ACHKIR_004203"
     CDS             10238..10564
                     /gene="hdeB"
                     /locus_tag="ACHKIR_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HFO9991866.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            10628..11275
                     /gene="yhiD"
                     /locus_tag="ACHKIR_004204"
     CDS             10628..11275
                     /gene="yhiD"
                     /locus_tag="ACHKIR_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFO9991867.1"
                     /translation="MTAEFIIRLILAVIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLGHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNTVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(11327..12097)
                     /locus_tag="ACHKIR_004205"
     CDS             complement(11327..12097)
                     /locus_tag="ACHKIR_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein"
                     /protein_id="HFO9991868.1"
                     /translation="MISAQNLVYSLQGRRLTDNVSLTFPGGEIVAILGPNGAGKSTLL
                     RQLTGYLQPDSGECRLFNKPLNEWSITELAKHRAVMRQNSHMAFPFSVQEVIQMGRHP
                     HRTGNQDNETAQIMALCDCQALANRDYRQLSGGEQQRVQLARLLVQLWEPTPSPKWLF
                     LDEPTSALDIHHQQHLFRLLRQLVHERQFNVCCVLHDLNLAARYADRIVLMQKGKVIA
                     NGKPQDVLTQQALTMLYGADITVLKDPANHSPLIVLDH"
     gene            complement(12094..13086)
                     /gene="shuU"
                     /locus_tag="ACHKIR_004206"
                     /gene_synonym="chuU"
     CDS             complement(12094..13086)
                     /gene="shuU"
                     /locus_tag="ACHKIR_004206"
                     /gene_synonym="chuU"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000910402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease ShuU/ChuU"
                     /protein_id="HFO9991869.1"
                     /translation="MLKDSLSSARVFMGLSLLLLALVLFGASQGALKISFDALFDEEY
                     RDIWLNIRLPRVLLAVLVGAALATAGVIMQGLFRNPMADPGLLGVSSGSALMVGVAIV
                     LPFSFPVVLVLYEQMVFAIAGSLVVCTIIFLITQRHRDGSMMQLLLAGIAINALCGAA
                     IGILSYIGDEQQLRQLTLWMMGNLGQAQWPTLLVASSFILPAIIATTCLAGTLNLLQL
                     GDEEAHYLGVNVKRKRQQLLLVSSLLVGAAVSVSGIIGFIGLVIPHLIRMTTGANHRW
                     LIPCSALAGACLLLMADTLARTLVQPAEMPVGLLTSLLGGPYFMWLILRNRRIT"
     gene            complement(13135..13758)
                     /gene="chuY"
                     /locus_tag="ACHKIR_004207"
     CDS             complement(13135..13758)
                     /gene="chuY"
                     /locus_tag="ACHKIR_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobilin reductase"
                     /protein_id="HFO9991870.1"
                     /translation="MTPWLLFGAGGKGVGARTLELALAEQRPVVAVIRHADAATKLAQ
                     QGVQVFTGDACDASVVAAACRAAGPDALIISTMGGAQDYLAHRTVIDEAEKAGITRMI
                     LVTSLGCGDSWPFLSERAKAAFGQAVREKTLAESWLQTSQLDYAILRPGGLLDGAATG
                     KAQRIQNQECHGFINRADVAAHIHELANAPALNQQVYSLIEPDLKPA"
     gene            complement(13758..14252)
                     /gene="hutX"
                     /locus_tag="ACHKIR_004208"
     CDS             complement(13758..14252)
                     /gene="hutX"
                     /locus_tag="ACHKIR_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme utilization cystosolic carrier protein
                     HutX"
                     /protein_id="HFO9991871.1"
                     /translation="MSHVSLQEFLKTEPDGTLEAIAEQYNTTLLEVVKNLPSPTVVSG
                     DKFDTVWDTVCEWGNVTTLVHTTDVILEFSGELPSGFHRHGYFNLRGKHGMSGHIKAE
                     NCTHIALIERKFMGMDTASILFFNKEGSAMLKIFLGRDDHRQLLSEQVSAFHALAASL
                     KEHA"
     gene            complement(14265..15602)
                     /gene="hutW"
                     /locus_tag="ACHKIR_004209"
     CDS             complement(14265..15602)
                     /gene="hutW"
                     /locus_tag="ACHKIR_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme anaerobic degradation radical SAM
                     methyltransferase ChuW/HutW"
                     /protein_id="HFO9991872.1"
                     /translation="MNTNNTLDLTPHFALDGDQPFKDRRAMMPFRGAIPVAKEQLTQT
                     WQEMLNQTVSPRKRLVYLHIPFCATHCTFCGFYQNRFNDDACAHYTDALIREIEMEAD
                     SVLHQSAPIHAVYFGGGTPSALSAHDLARIITTLREKLPLAPDCEITIEGRVLNFDAE
                     RIDACLDAGANRFSIGIQSFNSKIRKKMARTSDGPTAIAFMESLVKRDRAAVVCDLLF
                     GLPGQDAQTWGEDLAIARDIGLDGVDLYALNVLPNTPLGKAVENGRTTVPSPAERRDL
                     YLQGCDFMDDAGWRCISNSHWGRTTRERNLYNLLIKQGADCLAFGSGAGGSINGYSWM
                     NERNLQTWHESVAAGKKPLMMIMRNAERNAQWRHTLQSGIETARVPLDELTPHAEKLA
                     PLLAQWHQKGLSRDASTCLRLTNEGRFWASNILQSLNELIQVLNAPAIALEKP"
     gene            complement(15622..16536)
                     /gene="shuT"
                     /locus_tag="ACHKIR_004210"
     CDS             complement(15622..16536)
                     /gene="shuT"
                     /locus_tag="ACHKIR_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312409.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     ShuT"
                     /protein_id="HFO9991873.1"
                     /translation="MPRIITRPFLFSPLTLCISAVASAAKTAVKRKKLFTAVLALSWA
                     FSVTATERIVVAGGSLTELIYAMGAGERVVGVDETTSYPPETAKLPHIGYWKQLSSEG
                     ILSLRPDSVITWQDAGPQIVLDQLRAQKVNVVTLPRVPATLEQMYANIRQLAKTLQVP
                     EQGEALVTQINQRLERVQQNVAAKKAPVKAMFILSAGGSAPQVAGKGSVADAILSLAG
                     AENVATHQQYKSYSAESLIAANPEVIVVTSQMVDGDINRLRSIAGITHTAAWKNQRII
                     TVDQNLILGMGPRIADVVESLHQQLWPQ"
BASE COUNT         4339 a         3920 c         3962 g         4323 t
ORIGIN      
        1 gcttatctgg cggatgaaat cgccaaactg gggccgtatg agttcatctg taccggtcgc
       61 ccggacgaag gcatcccggc ggtttgcttc aaactgaaag atggtgaaga tccaggatac
      121 accctgtacg acctctctga acgtctgcgt ctgcgcggct ggcaggttcc ggccttcact
      181 ctcggcggtg aagccaccga catcgtggtg atgcgcatta tgtgccgtcg cggcttcgaa
      241 atggactttg ctgaactgtt gctggaagac tacaaagcct ccctgaaata tctcagcgac
      301 cacccgaaac tgcagggtat tgcccagcag aacagcttta aacacacctg ataacatcac
      361 gttgtaaaaa ccgaatgccc aacctttaaa aacaggtggg tattcggcta cttattaatt
      421 gcaataatat attacctgtc tattcatcgt gttgataaga tatatgttat atgaatgtta
      481 tggagcatag cgatactgct taatattacg atgatgaatt atttgcttac tattaattcc
      541 tctgcgttct taagtcccct ttttatataa taaatccagt catccagccc gagtatcatc
      601 gctcattatc atcctgtttt cccgcttcgt ttaaatttat ttatcaatca atttgactta
      661 agagggcggc gtgctacatt aataaacagt aatatgttta tgtaatatta agtcaactat
      721 gcaaccacta catggtaatt gtctaattgc gtacgcaaga cataaatata ttctcaccat
      781 ggttaatggt gaatatcgct attttaatgg cggtgatctg gtttttgcgg atgcaagcca
      841 aattcaagtg gataagtgtg ttgagaattt cgtattagtt tcaagagaca cgctttcatt
      901 atttctcccg atgctcaagg aggaggcatt aaaacttcat gtacataaaa aagttccttc
      961 attactcgtt catcactgta ccagagatat tcctgttttt caggaagttg cgcaactatc
     1021 gcagaataag aatcttcgct atgcagaaat gctacgtaaa agggcattaa tctttgcgtt
     1081 gttatctgtt tttcttgagg atacgcagtt tataccgtta cttctgaacg tgttgcaacc
     1141 gaatatgcga acacgagttt gtacggtcat caataataat atcgcccatg agtggacatt
     1201 agcccgaatt gctagcgaat tgttgatgag tccaagcctg ttaaagaaaa aattgcgcga
     1261 agaaggtacg tcatattcac agttgcttac tgagtgtagg atgcagcgtg ctttacaact
     1321 tattgttata tatggggttt caattaagcg agttgcagta tcctgtggat atcacagcgt
     1381 gtcgtatttc atttacgttt ttcgaaatta ttatgggatg acgcccacag agtatcagga
     1441 gcgatcggcg caggaattac cgaactgtgg ctcggcggca agtatggctg cgcaagggat
     1501 tttttacggc actgaccgtt ctgcggaagg aataagatta tagagtttta ctcagacata
     1561 aaaaaaaccc ggcatagggg accgggaaga ggatagtctg ccgtctccag actaataaac
     1621 cgttataaca ctccctgttg gcacgggaaa ctttgtgctc tcagtaagtt aaatataact
     1681 tttactggaa ataagatcag ccattttttt ataaacataa gctatacgct gtgtgaaaat
     1741 ataatcgtgt tgcatttact tattatcaat taactgttat gcaaaactac tttgtggata
     1801 aaatttggtc ctaccaaata tggcagtttt tgcacacaga aacagtctgg cgtcatttca
     1861 ttagtatact gacattgaaa taatcgcagt aatgaaatat aagggatagt catggctcat
     1921 gtctgctcgg tgatcctcgt tcgtcgttca ttcgatattc atcatgaaca gcaaaaaata
     1981 tcgttgcata acgagagtat cctgctgctg gataaaaatt tggcagccga ttttgcgttt
     2041 tgttcactgg atacgcgacg gctggatatc gaagagctga cagtttgcca ttacttacaa
     2101 aatattcgtc agttgccacg caatttagga ttgcatagca aagaccgttt gttaattaac
     2161 cagtcacccc ccatacagct ggtgacggcg atttttgata gtttcaatga cccccgggtc
     2221 aattcgccga tactgagcaa aatgctctat ctttcctgtt tatcaatgtt ttctcataag
     2281 aaagaactga tccccttact tttcaatagt atcagtactg tttcaggaaa agttgaacgc
     2341 cttattagct ttgatatcgc taaacgttgg tatctacgcg atatcgcaga aagaatgtac
     2401 accagcgaga gtctcatcaa aaaaaagttg caggatgaaa atacctgttt cagtaaaata
     2461 ttactcgcct ccaggatgtc gatggccaga cgattactcg agttacgtca aatacctctg
     2521 catactattg cggaaaaatg tggctatagc agtacgtcat actttataaa tacatttaga
     2581 caatattatg gtgtaacgcc acatcagttt tcgcaacatt cgccgggtac cttttcctga
     2641 catatttcgc atttgaatat tggtcaggat ctcacgtctg cttcatgtga aactctttcc
     2701 tgatgatttc tgccgggcta ccggctagtt ctctttcgca gtttgccagg cggtataggt
     2761 attttcagga ggaaaagact cggcatgttt gggattatta agctaacaat tcatacgatt
     2821 acgggaatgt gggtcagtat tgtgctgttc aaattgatga cgaatggctg gagtggtttc
     2881 tactttcagt gctgtgtact aagcctggta ttcctgacgg ttagctggtt gttgagtggt
     2941 gaatggttag caggaaagag taaggctgaa ccctcacgtt caaccttact ctcatttaca
     3001 cgttacgctt ttttaaagcg ggcaaagaga tgttccacta caacgaaaaa gaccggaacg
     3061 aagtaaattg ccagcactgt tgcggcaaac atcccgccca ttacgccggt acctaccgcg
     3121 ttttgcgcgc cagagccggc accatgactg ataaccagcg gcagcacgcc gagaataaag
     3181 gccagagaag tcatcaggat tgggcgtaaa cgcatacgcg ctgcttcgat gattgcctct
     3241 accggcgttt tcccttcttt ctgcatcatc tcaacggcaa attcgacaat caggatggcg
     3301 ttcttggcgg aaagcccgat ggtggtcagc aaaccaacct ggaagtaaac gtcattactt
     3361 aagccgcgca gatcggtggc cagtaatgcg ccaacgacgc ctaacggaac aaccaacatc
     3421 accgagaacg gaattgacca gctttcataa agtgcggcga gggcgaggaa caccacgacc
     3481 agtgaaatgg catatagcgc gggagcctgg tttgaggata acgcttcctg atacgacagc
     3541 ccggtccatg agtagccgac gcctgccgga agttttgcga ccaggtctgc cataaatttc
     3601 atggcgtcac cggtactttt cccggcagcc gcttcaccta aaatctccat tgacgggatg
     3661 ccgttgtagc gttccagtcg cggtgaacca taggtccatt cggtagacga ataggcagaa
     3721 agcggtgcca tcgtgccaga ggcgttgcgt acataccatt ggttgatgtt atccggcaac
     3781 atacggaacg gcgtgcctgc ctggacatac acttttttca cccgcccctg gttgaggaag
     3841 tcgttcacgt agctgctgcc gaaggcggtg gaaattgtct ggttgatatc agacagcgcc
     3901 acacccatag cctcggcttt cgcagcgttg acgttcactt tgaacatcgg cgtatcttcc
     3961 aggccgttcg ggcgtacccc gatgacctga ttcggtgatt gcgctgccag tgataacagc
     4021 tcgtttcgcg cctgggtcag cttttcgtgc cccagattgc cgttgtccag cagttccata
     4081 tcaaaacctg acgcggtacc cagttcagcc accgcgggta agttgaacgg gaagacgacg
     4141 gctttattga tactgcttag cgcaatcatt gcccgctgaa taatcgcggt aaccgagttt
     4201 tcctcgccga cacgttcaga ccacggcttg aggctgataa acgccagacc gttgttttgc
     4261 ccctgaccac tgaagccaaa gccaccaacg gtaaacaccg actgaacatt atttttctct
     4321 tttgtcagat aataatccgt cacctgttgt agcactttcg tggtgttaac catggttgca
     4381 ccggaaggta actgcgcggt ggtcataaat accccctgat cctcttctgg taagaaagag
     4441 gtcggcgtac gcaggaacag caccgccatc ccggcgcaaa tcagcaggta gaccaccatg
     4501 tagcgaccgg tacaacgcaa cagcgagcgg gtgctgtcgg tatagtgctg agttgatttt
     4561 tcaaacagcg tgttgaagcg tgcgaacagg gcgttaggtt tgtgatcgcc ctccggcgcg
     4621 gctttcagaa tggtggcgca cagggcaggg gtcaggctca ttgctacaaa tactgaaagc
     4681 agcatggagg agatcagcgt gatggagaac tggcggtaga tctccccggt cgcaccgctc
     4741 ataaaggcca tcggcataaa cactgcggaa agaacaacgg caatcccgac cagcgcacgt
     4801 tggatctgcc ccatcgattt atgcgtcgct tccttcggcg gtagcttatc ttccgcaatg
     4861 acacgctcga cgttctccac caccacgatg gcgtcatcca ccagtaaccc tatcgccagc
     4921 accatcccga acatagttaa cgtgttgatg gtgaaaccga ccgccgacaa gatcgcaaac
     4981 gtcccgagaa taaccaccgg tacggcaatc gtcgggatga ttgtggcgcg gaaattctgc
     5041 aaaaacagat acatgaccag gaagacgagg atgatagcct caaccagcgt tttgaaaact
     5101 tcctgaatag aaattttgat aaacggtgtg gtgtcgtaag gataaaccgt cttcaggctt
     5161 gcagggaaat aagccgataa acggttcagc tcctctttca ccgctcgcga ggtatccagg
     5221 gcgtttgctc cggtagccag tttgatggcg atcccggcag caggtttgcc gttatagcgc
     5281 gccacggtgg aataatcttc cgccccaagt tcgacgcggg cgacatcacg cagcagtact
     5341 tgcgaaccat cttgctgaac tttcaacagg attttgccaa attcttccgg cgtttgcaga
     5401 cgcgtctgca caatgatcga ggcgttcagt tgctggtctg ccgcctgtgg catgccaccc
     5461 agttgaccac cggaaatctg gttattttgc accttaatct gggaaataac atcggaaggt
     5521 accaggttat atttattgag tttttgcggg tccagccaga tacgcatggc gtactcggaa
     5581 ccaaagagct gtacgctacc aacgcccgcg gtacggctta gcgggtcttt gatattagac
     5641 gctacatagt ccgcgatatc gtactggttg aggctgccgt tatcagaaat aaatgccgct
     5701 accatcagga tattactgct cgacttatcg acgctaatcc cctgctgctg caccgcttca
     5761 ggtaatgaag gcatagcgag ctgcagttta ttttgcacct gaacctgtgc gatatcagga
     5821 gatgtcccag tctcgaaggt cagagtgata gaggcattac ccgccgcatc actggttgaa
     5881 gacatgtaca tcaggccatc aagcccattc atattttgct caatcacctg agtgaccgag
     5941 tcttctaccg tttgcgcatc ggcacccgga taggttgcgc tgacggtaat ggtcggtggc
     6001 gcaatctgcg gatactgcgc aaccggtaag ttcatgatcg ccagaccacc tgcaagcatc
     6061 ataataatgg caagtaccca ggcaaaaacc gggcgatcaa taaaatagtt agccatgaaa
     6121 gtccccttaa gcctgcaacg ttattgtttc gattcggtgc tggcgttttc ctggctggag
     6181 gaaattgctc gtgctttgat acccggacga atgcgttgca aaccggaaac gatcacccga
     6241 tcgccagcct gcaagccaga ggtgacaacc cactggtcgc cgatggcttt gctggcttca
     6301 atttcgcgta gctgcacaac atcgtcttta tccagaatga gcgccgttgc tttaccctgg
     6361 gcgttgtggg tgacgccttc ctgcggcacc agtaatacat tctggcggct accttcatcc
     6421 actaatgccg tgacgtacat gccaggcagc aagtcaccat ttgggttggg gaaaatcgcc
     6481 cgtaacgtca cggagcccgt ggtttcatcc accgtcgggt cggagaattt cagcgtgccg
     6541 gtctggctgt aacgtttacc attttccaga ttgagctgta ctggcgtact gccctgaacc
     6601 tgtttgattt gcccactggc gacctcttct ttcatgcgca agaaatcctg caccgactgc
     6661 gtgagatcga cataaatcgg gtccagacgt tgtacggtaa ccagcgaatc tgcctgatta
     6721 gcggtaacga gtgcgccgac ggtcaccgac gatttcccgc tgacgcccgt aatcggcgag
     6781 gtgacatttg cgtattgcag gttgatagtc gcctgttcaa cagccgcttt ggcgacggtg
     6841 acattggctt ctgcttcatt caactgggtg cgcgcggtgt cgtaatcctg acggctaacg
     6901 tagttggtct tcagcaacga tgcctggcgg ttaaaggtga tgcgggcatt gctggcggta
     6961 gagagcgctt tcgccagcga gcctttggcg gagttcagct ctgcctgtaa aggcgcagga
     7021 tcaatctgat acagcgaatc gccctggttc actttatcgc cttcgataaa gttgcgttta
     7081 atgataatac cgcccacctg gggacgtatc tcggcaactt cataaggaac ggttctacca
     7141 ggcaattcgc tcaacacatt gaccgaaccg ggggagagtg tgacgacacc gacctcaggc
     7201 gtcatggcgg cggcgttttc cgccgatttg tcatcgcagg cggtgagcat cgcgccgcag
     7261 aataacaacg gtattaacag ctttcttctt ctgttcattt tagtccctga aaattcttga
     7321 gaaataacgg ttctttaaat tgcgcgcgag gagagatatg agggatttac agaccagcgg
     7381 catagtgggg ttgtcagcca gtaaggtggg atacaggcac agtgatcgac atggtgaggt
     7441 caacgacaaa aaaaatgttg ccgttctgcc aacagttcct gccagcattc gggcaacgaa
     7501 gggttctact ggtggataca tataccaggg gaataaagtc gttttcgcct ctcctttagt
     7561 aatcaccggg ggtaatgaaa tcagcatctg catccctcgt catgccagcc atcaatttca
     7621 gttgcttatg tcctgactaa aaataagatg tgatacccag ggtgacgatg tcgctcatac
     7681 gtcttagttt cagtttgtac ataatgttga atttctgaat ctttaatgtt tgttgcgaaa
     7741 tattgagcgt cgacgtgata ttgctttcat ttttattttt cagaatcagt tgcataattt
     7801 tgaactgatt atgtgaaaaa cataattgtg aattcttatg gggcaagtgt ttaccataag
     7861 taatctcaag aagatttttg atactttctt tgcggctgac aaaataaata ggcgctttag
     7921 cttttagttt actgatgtga taaggtgcca aaacaataat tctctcggca tctaatttct
     7981 ccagaaattt aatcgcttct tcatcaagga tatgattgtg ataggtatca atgataacct
     8041 tgaaggggtt attgcctact tttttgatct ctgacaggga attgattttt atcatttcgt
     8101 gattatcttt caactgccaa aagccctgta aaagaaaaga atctttcgtc atgagaaaaa
     8161 tcataacttg ctccttagcc gttatcgttt cgaacatgtc atccttttca ttatttacat
     8221 ccttgtccga atcgttgttc aatatagtaa acgcctataa gaactttgcc cttcgtgaac
     8281 taatacgtct attaacacta atcaatactt cagatttatc catcacgcac tccatatatt
     8341 ggtcctaagt aagattcctg gttgttatca gtttgtaact agtaaacaaa aggttaactt
     8401 ttgcggatta ctgcccaaga ataagttatc aataaccagt ttgtgatctc tgaagaatat
     8461 tactaaagtt aaaatgtaat ccgatttaaa tatcgagtct ccttgtttcg acttaggctg
     8521 gcaattggat tgccagcttt cttttaacgc attgaaatta cctgattaaa taactcattc
     8581 atgtcttcag gtttttatat agcgtctgga gacacggata tttatgcaat gtaatcaacg
     8641 ccagttgcaa aaaaatgaac ccggattact tatttaaccg cccatagcat caacgcttac
     8701 ggaactcttt gttcactttc ccacagcact tttaaaacat taaacctgac cacggaaaat
     8761 atcagccatg ataatgtttg aaatggctaa tttgccatgg aacgaaaaaa atcagattaa
     8821 attcagtagg ttgaaataat caacagcagg caattgtttc aatgatagtg cgtaattgaa
     8881 ctgaaacact gtatagctaa gagagtatta actggatcat agtcacttaa tgtgactatg
     8941 atccaggtgg aaatagggat aattattgct ttttaacgaa caaactggcg aagctgaaca
     9001 gactggtggc gctaaatatc agttcaattc ccaccagtgt ggaaaccagc gttaccgaca
     9061 ccatcggcgt agcaccaagg aatatccagg caatgacgat atccagcaca ccaataacga
     9121 gctgtagcca actgcctttc attgaacgct gacgatacca actcatcagg cgaataaccc
     9181 ctgccacaca gaacaaaccg gcaataaatg ccgcaatggc aaaaatgccc agctctggtg
     9241 cgcggatgaa gaaatagccg atcaataaat aggcgactgc gacgaggaaa ccggataata
     9301 ccggccagaa attatgactg cggttgctga atagcccgac aataagcgca atgcccgagc
     9361 agattaataa tgcacccact actgtgctta aaatgtcgcc agagacgaac ggaaaactga
     9421 tacacagcaa cccgacgata aacagcagca cggcaataaa ctggattgct ctgcgatgtt
     9481 ttttaagcat ctccagatca aacttcaaaa ttgttgcctt atctatatat aacatagaac
     9541 caccctataa aattaagaag aaaatcccct gctatcaatc tatgccaaaa acgcgtgtaa
     9601 gaatgcagtc gatttaataa aaatttccta attacagtat ctgatgcgtc tgtaactcat
     9661 tgtattgaaa taaaaatatc tgattttgat attttccatc aacatgacat atacagaaaa
     9721 ccaggttata acctcagtgt cgaaattgat tcgtgacggc tctttcactt tatagttgag
     9781 gatattacga tgaaaaaagt attaggcgtt attcttggtg gtctgcttct tctgccagta
     9841 gtgagcaatg cagcggatgc acaaaaagca gctgataaca aaaaaccggt caactcctgg
     9901 acctgtgaag atttcctggc tgtggacgaa tccttccagc caactgcagt tggttttgct
     9961 gaagcactga acaacaaaga taaaccagaa gatgcggttt tagatgttca gggtattgca
    10021 accgtaaccc cagctatcgt tcaggcttgt actcaggata aaaaagccag ctttaaagat
    10081 aaagttaaag gcgaatggga caaaattaag aaagatatgt aattccggga atgcgttaca
    10141 tcgtatttcc ttgcatattg aacaggccgg aatatcttct ttaaaagcag ctattcctcc
    10201 tgtttgtata taatctctat attgaatggg ttacaaaatg aacatttcat ctctccgtaa
    10261 agcgtttatt tttatgggcg ctgtagcggc tttgtcactg gtgaacgcac aatctgcgct
    10321 ggcagccaat gaatccgcta aagatatgac ctgccaggaa tttattgatc tgaatccaaa
    10381 agcaatgaca ccagttgcgt ggtggatgct gcatgaagaa acagtatata aaggtggcga
    10441 taccgttact ttaaatgaaa ccgatctcac tcaaattcct aaagtgatcg aatactgcaa
    10501 gaaaaacccg cagaaaaatt tgtatacctt caaaaatcaa gcatctaatg acttgccgaa
    10561 ttaatgaggt acaagtaaaa aggagtagca agttgagcca tcttgctgct cctttttgca
    10621 tttttatatg acagcagaat ttattatacg acttatactt gcggtaattg cctgtggcgc
    10681 tattggcatg gaaaggcaaa tgcgcggcaa aggagcaggg ttacgcacac atgtattaat
    10741 tggcatggga agcgccctgt ttatgattgt ttcgaaatat ggttttgctg acgtgctgtc
    10801 tttaggtcac gtcggactgg accccagccg tattgctgct caggttgtga cgggcgtcgg
    10861 gtttatcggt gcaggtaaca ttctcgttcg taaccaaaat attgtaggtc tgacgacggc
    10921 agcggatatc tgggtgaccg ccgccattgg tatggttatt ggcagcggta tgtacgaact
    10981 gggtatttat ggttcagtga tgaccttgct ggtgctggaa gtcttccatc aattaacctt
    11041 ccgcctgatg aacaaaaatt accatttaca gctgacatta gtgaacggta atacggtatc
    11101 aatgctcgac tggtttaagc agcaaaaaat aaaaacggat ttagtttcgt tgcaggaaaa
    11161 tgaagatcat gaagtggtcg ccattgatat ccagcttcat gccacgacgt cgatagaaga
    11221 tctgttgcga ttattaaaag gtatggcagg ggtgaaaggc gtttcaatta gctaatctgt
    11281 atagttattt attcgcagga gtaccagtgc ggtactcctg ttggttttaa tgatcgagga
    11341 cgatcagggg tgaatggttg gcaggatctt taagtacggt aatatccgcg ccgtaaagca
    11401 tggtcagcgc ctgctgtgtc aatacgtcct gtggtttacc atttgcgata acctttcctt
    11461 tttgcattaa tacaatacgg tctgcataac gcgcggcaag atttagatcg tgcaatacgc
    11521 agcaaacatt gaactgccgt tcgtgaacta actggcgtaa taaacggaac aagtgctgct
    11581 gatggtgaat atccagcgcc gaagttggtt catcaagaaa taaccatttc ggtgagggag
    11641 ttggttccca gagttgcacc agcaggcgcg ctaattgtac gcgctgttgt tcaccaccgg
    11701 ataattgccg atagtcgcgg tttgctaacg cctgacagtc acataacgcc attatttgtg
    11761 ctgtttcatt atcctgatta ccagtgcgat gcggatgccg ccccatctgg ataacttctt
    11821 gtacgctgaa tggaaacgcc atgtggctat tttgacgcat cacggcccgg tgtttcgcca
    11881 gttcagtaat cgaccattca tttaatggct tattaaacaa ccgacattcg ccgctatcag
    11941 gttgaagata gccggttaac tggcgaagca gcgtagattt tcccgcacca ttagggccga
    12001 gaatggcaac aatttcccca ccgggaaaag tcagcgagac attatcagtc agacgccgcc
    12061 cctgaaggct ataaaccaga ttctgggcgc tgatcatgtg atcctccggt tgcgcagaat
    12121 caaccacata aaataaggac caccaagcag gctggttaat aatcccacgg gcatttctgc
    12181 tggctgtacc agcgtgcggg caagcgtgtc tgccatcagc aataaacagg ctccggcgag
    12241 ggcggagcaa gggattagcc agcggtgatt tgctccggta gtcatgcgaa tcagatgcgg
    12301 gatcaccagg ccaataaagc cgataatgcc gcttaccgat acggcggcac caacgagcag
    12361 tgagctcact aacagtaatt gctggcgttt acgcttaacg ttcacgccga ggtaatgggc
    12421 ttcttcatca ccgagctgca gtaaattcag cgttccggcg agacaagttg ttgcgataat
    12481 ggccggtagg atgaatgaac tggcaaccaa taacgtcggc cattgcgcct gtccaagatt
    12541 gcccatcatc cacaatgtga gttgtcgtag ctgctgctca tcgccgatat agctcaggat
    12601 gccgatcgct gcgccgcaca gggcattgat ggcgataccg gcgagtaata attgcatcat
    12661 gctgccatcg cgatggcgct gcgtgatgag aaaaatgatg gtgcagacca ctaaacttcc
    12721 ggcaatggca aacaccattt gctcatagag caccagcacc actgggaagg agaagggcag
    12781 cacaatagca acgccaacca ttaatgcaga accgctactg acgccaagta atccagggtc
    12841 agccattggg ttgcgaaaaa gcccctgcat aattaccccg gcggttgcca acgctgcacc
    12901 taccagcacc gccagcaaaa cccgtggtag acgaatattg agccagatat cgcggtattc
    12961 ctcatcaaaa agggcatcaa aactgatctt taacgctccc tgactggcac caaacagcac
    13021 cagagctagc aataatagtg atagccccat aaagaccctg gcggaagaga gtgaatcctt
    13081 gagcacgcaa aactccgtta cgacattacg gcgcggacat gccgcgccgg gggatcacgc
    13141 cggtttcagg tccggttcaa tcagactgta gacctgctgg ttaagtgctg gcgcatttgc
    13201 cagttcatgg atatgtgcgg cgacatcagc acggttaata aaaccatgac actcctgatt
    13261 ttgtatccgt tgtgctttgc ccgttgccgc accatcaagc agaccgccag ggcggagaat
    13321 ggcataatca agctggctgg tttgcaacca gctttccgcc agcgtttttt cccgtaccgc
    13381 ctgaccaaac gcagcctttg cccgctctga taaaaacggc cagctatcgc cgcatcccag
    13441 tgacgtcacc agaatcatgc gtgtgatgcc tgctttctcg gcctcgtcaa tcactgttct
    13501 gtgcgccaga taatcctgag ctccgcccat tgttgagatg ataagcgcat ccgggccagc
    13561 tgcgcggcaa gcagccgcga ctacgctggc atcacaggcg tcgcctgtaa aaacctgtac
    13621 gccttgttgc gccagtttcg tggcggcatc ggcatgacga atgacagcaa cgaccggacg
    13681 ttgttccgcc agcgccagtt caagcgttct ggcacccaca ccttttccgc ctgcgccaaa
    13741 gagtagccac ggtgtcatca ggcgtgttcc tttaacgatg ctgccagagc atggaaggca
    13801 ctgacttgtt cgctcaggag ctgacgatga tcatccctac cgaggaagat tttcagcatt
    13861 gcgctgcctt ccttattgaa aaagagaata gaggcggtat ccatgcccat aaatttacgt
    13921 tcgatcaagg caatatgcgt gcagttttct gctttgatat gcccggacat gccgtgcttt
    13981 ccgcgtaggt tgaaatagcc gtggcgatgg aaacccgaag gcagttcgcc gctaaattcg
    14041 agtattacat cggtagtatg tactaacgtg gtgacgttgc cccattcgca gacggtatcc
    14101 cagacagtat cgaatttatc acctgacaca acagtcggag agggcagatt tttcactacc
    14161 tccagcagcg tggtgttgta ttgttcggct atcgcctcca gcgtaccgtc tggctccgtt
    14221 ttcaaaaatt cctgtaacga gacatggctc ataaatcact cctgttatgg tttttcaagc
    14281 gcaattgctg gcgcattaag tacctgaatc agttcattaa gagactgcaa aatgttgctt
    14341 gcccagaaac gaccttcatt ggtcaaccgc agacacgttg acgcatcgcg gcttaagcct
    14401 ttttggtgcc actgggccag taacggcgcg agtttttcgg catgtggcgt aagttcgtcc
    14461 agcggtacac gcgccgtttc aatgcccgac tgcaaggtat gacgccactg cgcgttacgt
    14521 tcggcgttac gcatgatcat catcagcggt tttttacctg ctgcgacgga ttcatgccag
    14581 gtctgaagat tgcgttcgtt catccaggag taaccattaa tcgaaccgcc ggctccggaa
    14641 ccaaaggcca gacaatcggc accttgttta atcagcaggt tatagagatt gcgttcgcgt
    14701 gtggtacggc cccagtggct gttactgatg cagcgccaac cggcatcgtc cataaaatca
    14761 cacccttgca ggtaaagatc gcgacgttct gccggagagg gcacggtagt acgcccattt
    14821 tccacggctt tgcccagcgg tgtattgggc aggacattga gcgcatagag atcgacgccg
    14881 tcgagaccga tatcgcgggc aatagccaga tcttctcccc aggtttgcgc atcctgaccc
    14941 ggcagaccaa acagcaggtc acagaccacc gcggcacggt cgcgtttgac caggctttcc
    15001 ataaacgcaa tagcggttgg gccatccgag gtgcgggcca ttttcttgcg gattttgctg
    15061 ttaaacgact gaataccaat tgagaagcgg tttgctccag catcaaggca agcatcgatt
    15121 cgctcagcgt caaaattcag tacccggcct tcaatagtga tttcacaatc aggggccagt
    15181 ggcagctttt ctcgtaatgt ggtgatgatt ctggccaaat cgtgtgctga aagcgcggaa
    15241 ggcgtaccgc caccgaaata gaccgcgtgg atgggggcag actgatgcaa tacgctgtct
    15301 gcttccattt ctatttcacg aatcagggcg tcggtataat gagcacacgc atcgtcatta
    15361 aaacggttct gataaaaacc acaaaacgtg cagtgtgtcg cacaaaaagg aatgtgtaga
    15421 taaaccagtc gcttacgtgg tgataccgtt tgattgagca tctcttgcca ggtctgtgtc
    15481 agttgctctt tggcaactgg aatggcccca cgaaacggca tcatggcgcg cctgtcttta
    15541 aatggctgat caccgtctaa agcaaaatgt ggcgtgagat ccagggtgtt atttgtgttc
    15601 attcgtctga tttaaatcca tttattgcgg ccaaagctgc tggtgtaaag actcaacgac
    15661 atcagcaata cgcggaccca ttcccagaat caggttttga tcaacggtga taatgcgctg
    15721 gtttttccat gcggcagtgt gggtaattcc ggcaatagaa cgtagccgat taatatcgcc
    15781 atcgaccatt tgtgaggtga cgacaatcac ttcagggtta gccgcaatca gcgattccgc
    15841 gctgtagctt ttgtactgct ggtgggtagc gacgttttct gctccggcaa gcgaaagaat
    15901 ggcgtccgcg acgctgcctt taccggcaac ctgtggcgca cttccgcctg cggagagaat
    15961 aaacatggct ttaaccgggg cttttttggc cgccacgttt tgctgtactc gctccaggcg
    16021 ttggttgatc tgtgtcacca gcgcctcgcc ttgttcagga acctgtaacg tttttgccag
    16081 ttggcgaatg ttggcgtaca tctgctcaag ggtggctggt acacgcggca gagtgacgac
    16141 gttgactttt tgcgcccgca gttggtcgag cacaatttgc ggtcctgcat cctgccaggt
    16201 aattacgcta tccgggcgaa gtgacaaaat gccttcgctg ctgagttgtt tccagtaacc
    16261 aatatgcggc agtttggcgg tttctggtgg ataagatgtc gtttcatcga caccaaccac
    16321 gcgttcgcca gcgcccatcg cgtagattag ctccgtcagc gatcctcctg cgaccacgat
    16381 acgttcggta gccgttacgc taaaagccca gctaagagcc agtactgccg taaacaattt
    16441 ttttcgtttt accgcggtct tcgcggcaga ggccacggct gaaatacata acgtcagtgg
    16501 ggaaaaaaga aacggcctgg tgatgatcct tggcataata tgag
//
LOCUS       DAZGQL010000112        15260 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.112, whole genome shotgun sequence.
ACCESSION   DAZGQL010000112 DAZGQL010000000
VERSION     DAZGQL010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15260
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.112"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            315..760
                     /locus_tag="ACHKIR_004211"
                     /pseudo
     CDS             315..760
                     /locus_tag="ACHKIR_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611788.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4/IS5 family transposase"
     gene            804..1072
                     /locus_tag="ACHKIR_004212"
                     /pseudo
     CDS             804..1072
                     /locus_tag="ACHKIR_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008364.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1277..2395
                     /gene="ybdK"
                     /locus_tag="ACHKIR_004213"
     CDS             1277..2395
                     /gene="ybdK"
                     /locus_tag="ACHKIR_004213"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HFO9991874.1"
                     /translation="MPIPDFQVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRSLMEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            2461..2709
                     /gene="ybdJ"
                     /locus_tag="ACHKIR_004214"
     CDS             2461..2709
                     /gene="ybdJ"
                     /locus_tag="ACHKIR_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HFO9991875.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            2774..3142
                     /gene="ybdF"
                     /locus_tag="ACHKIR_004215"
     CDS             2774..3142
                     /gene="ybdF"
                     /locus_tag="ACHKIR_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFO9991876.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            3236..3889
                     /gene="nfsB"
                     /locus_tag="ACHKIR_004216"
     CDS             3236..3889
                     /gene="nfsB"
                     /locus_tag="ACHKIR_004216"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HFO9991877.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQVKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKMLDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDEEFGLEEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            3997..5244
                     /gene="ybdG"
                     /locus_tag="ACHKIR_004217"
     CDS             3997..5244
                     /gene="ybdG"
                     /locus_tag="ACHKIR_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HFO9991878.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIFLHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(5325..6701)
                     /gene="pheP"
                     /locus_tag="ACHKIR_004218"
     CDS             complement(5325..6701)
                     /gene="pheP"
                     /locus_tag="ACHKIR_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HFO9991879.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(6803..9946)
                     /gene="cusA"
                     /locus_tag="ACHKIR_004219"
     CDS             complement(6803..9946)
                     /gene="cusA"
                     /locus_tag="ACHKIR_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HFO9991880.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGQTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(9958..11181)
                     /gene="cusB"
                     /locus_tag="ACHKIR_004220"
     CDS             complement(9958..11181)
                     /gene="cusB"
                     /locus_tag="ACHKIR_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HFO9991881.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFAKAEPPAEKTSTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGTPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWITAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADESLKPGMNAWLQLNTASEPMLLIPSQALID
                     TGSEQRVITVDADGRFVPKRVAVFQASQGVTALRSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATHAH"
     gene            complement(11197..11529)
                     /gene="cusF"
                     /locus_tag="ACHKIR_004221"
     CDS             complement(11197..11529)
                     /gene="cusF"
                     /locus_tag="ACHKIR_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HFO9991882.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVV
                     KTIDLESKKIIIHHDPITAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(11553..12935)
                     /gene="cusC"
                     /locus_tag="ACHKIR_004222"
     CDS             complement(11553..12935)
                     /gene="cusC"
                     /locus_tag="ACHKIR_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HFO9991883.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPAMPVPQQFSLNQNGLVN
                     AADNYQNAGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSNNLKGDSATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSDSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGGWQQ"
     gene            13092..13775
                     /gene="cusR"
                     /locus_tag="ACHKIR_004223"
     CDS             13092..13775
                     /gene="cusR"
                     /locus_tag="ACHKIR_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HFO9991884.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            13765..15213
                     /gene="cusS"
                     /locus_tag="ACHKIR_004224"
     CDS             13765..15213
                     /gene="cusS"
                     /locus_tag="ACHKIR_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HFO9991885.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQAQRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFTRDAIPDKDTRGGEVYLLSGPTMMMPGHGHGHMEHSNWRMISLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASIISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDECQVAGDPLMLRRALSNLLSNALRYTP
                     PGEAIVVRCQTVDHQVQVTVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFMIKLPEKG"
BASE COUNT         3702 a         4093 c         3741 g         3724 t
ORIGIN      
        1 acgtgcaacg aattttaagt gtttcttgag tgacataaaa taagtgtggt gtccatatcc
       61 tcctatcgtg aatcaatata aatgatttac acctaacact ataaaatgtt cagaaagcca
      121 tcgcaataat aaaaaataag acattacgat aatattataa agcattgctt tgttggggga
      181 tgacgtatgt agcaatgaaa gataacaaat gccggtaatg gtacggagaa atcctcagta
      241 ataggcgggt aaaaaagacg ggcatagagt tttgtggtcg actggttgtg ctaacaattc
      301 aggagatcac ctctatgccc gttcgctcat catgccaaaa gtttttccgt gatgatttga
      361 ctccatttca tcagtaccgc cagaatgcca tccttgacgc cactgctgcg cttagtcggg
      421 acgcatccct catccttacc agcattggac ggtatatgcc aggcaatgct caggttaaaa
      481 caagattaag cccatcgatc gcctgccagg caatgacgct ttacaccgcg atatcccggc
      541 tatttttcat aatattaccc ggtttcagcc ccgagacctt acgttgtgcg tcattgccgt
      601 tgactggagt ggttatcctt ctcaggcatt ccatgttctg catactcccc aagtttgcga
      661 cgggcgttct atattacttc tggatcaggt tgtatcgtca ttctggcaac aaattgctca
      721 gttctataag aattttcttg atacgcttgc aatcactatt gctccgaata aaccggataa
      781 aaattacctt taaacacagc accatgaccg ccacaagggc atatttcgtc agcatgttta
      841 tattgcctcc atgcaaagaa gggactacca tccaaattgt ttgggtttgg agtggcagcc
      901 ccaggattga tagaaatatc tcctggggct tcatctttcc agacctcaga gtaaacctga
      961 aaccggaaag cttcaggcac ccgccgttat cttacccttc cttagcaaaa tggctgttta
     1021 tttatacagt aagtcctgtt tcggacttct caatcctcat ttgatcaact gaagctgaac
     1081 ccgttttatt ctgcatgcct caatcgtcaa aattcttcgc caccagtccg cactgccata
     1141 acttgtcata ttgcaaaatg tagccaattt gcttcagtca ccatcatcaa aaaaagaaac
     1201 attccagaac ttccgcatct cgcccagccg ctccgacttc tatactgaat agaaaacgcc
     1261 aacataagag aaacctatgc caatacccga ttttcaagtc tctgaacctt ttaccctcgg
     1321 tattgaactg gaaatgcagg tggttaatcc gccgggctat gacttaagcc aggactcttc
     1381 aatgctgatt gacgcggtta aaaataagat cacggccgga gaggtaaagc acgatatcac
     1441 cgaaagtatg ctggagctgg cgacggatgt ttgccgtgat atcaaccagg ctgccgggca
     1501 attttcagcg atgcagaaag tcgtattgca ggcagccgca gaccatcatc tggaaatttg
     1561 cggcggtggc acgcacccgt ttcagaaatg gcagcgtcag gaggtatgcg ataacgaacg
     1621 ctatcaacga acgctggaaa actttggcta tctcatccag caggcgaccg tttttggtca
     1681 gcatgtccat gttggctgcg ccagtggcga tgatgccatt tatttgctgc acggcctgtc
     1741 acggtttgtg ccgcacttta tcgccctttc cgccgcgtcg ccatatatgc aaggaacgga
     1801 tacgcgtttt gcctcctcac gaccgaatat tttttccgcc tttcccgata atggcccgat
     1861 gccgtgggtc agtaactggc aacaatttga agccctgttt cgctgcttga gttacaccac
     1921 gatgatcgac agcattaaag atctgcactg ggatattcgc cccagtcctc attttggcac
     1981 ggtggaggtt cgggtaatgg ataccccgtt aacccttagc catgcggtaa atatggcggg
     2041 attaattcag gctactgccc actggttact gacagaacgc ccgttcaaac atcaggagaa
     2101 agattacctg ctgtataaat tcaaccgttt ccaggcctgt cgctatgggc tggaaggcgt
     2161 cattaccgat ccgcatactg gcgatcgtcg gtcactaatg gaagacacct tgcgtttact
     2221 ggaaaaaatc gccccttctg cacataaaat tggcgcatcg agcgcaatag aagcactgca
     2281 ccgccaggtc gtcagcggtc tgaatgaagc gcagctgatg cgcgatttcg tcgccgatgg
     2341 cggctcgctg attgggctgg tgaaaaagca ttgtgagatc tgggctggtg actaagccgg
     2401 gaattgcctt acagaagaat atccctgaca atggtgtttt aacttacatt taacaatgtt
     2461 atgaaacacc ctttagaaac cttgaccacc gcagcaggca ttttgctgat ggctttcctt
     2521 tcttgcctgc tgctgcccgc ccccgcactg gggctgacgc tggcacaaaa actggtgacc
     2581 atgttccatc tgatggatct tagccagctt tacactttat tgttttgtct gtggttttta
     2641 gtgctgggcg ctattgagta ttttgttctg cgctttatct ggcgacgctg gttctcgctg
     2701 gcggattaaa cgtcgattat cgccgtactt cgcggcatac tttgcttatt ctccttcgcc
     2761 taaaggaacg tttatggata agcaatcact gcacgaaacg gcgaaacgcc tggcccttga
     2821 gttacccttt gtcgagcttt gctggccttt tggcccggag ttcgatgttt ttaaaattgg
     2881 cggcaagatt tttatgctgt cgtcggagct gcgcggcgtc ccctttatca atctgaagtc
     2941 cgatccacaa aaatccctgt taaatcagca aatatacccg agcatcaagc cagggtatca
     3001 catgaataaa aagcactgga tttcagtgta tcccggcgag gaaatctccg aagcgttact
     3061 tcgcgatctg atcaacgatt cgtggaatct ggtggttgat ggtctggcta aacgcgatca
     3121 aaaaagagtg cgtccaggct aaagcggaaa tctatagcgc atttttctcg cttaccattt
     3181 ctcgttgaac cttgtaatct gctggcacgc aaaattactt tcacatggag tctttatgga
     3241 tatcatttct gtcgccttaa agcgtcattc cactaaggca tttgatgcca gcaaaaaact
     3301 aaccccggaa caggccgagc aggtcaaaac tctcctgcaa tacagcccat ccagtaccaa
     3361 ctcccagccg tggcatttta ttgttgccag cacggaagaa ggtaaagcgc gtgttgccaa
     3421 atccgctgcc ggtaattacg tgttcaacga gcgtaaaatg cttgatgcct cgcacgtcgt
     3481 ggtgttctgt gcaaaaaccg cgatggacga tgcctggctg aagctggttg ttgaccagga
     3541 agatgccgat ggccgctttg ccacgccgga agcgaaagcc gcgaacgata aaggtcgcaa
     3601 gttcttcgcc gatatgcacc gtaaagatct gcacgatgat gcagagtgga tggcaaaaca
     3661 ggtttatctc aacgtcggta acttcctgct cggcgtggcg gcgctgggtc tggacgcggt
     3721 gcccatcgaa ggttttgacg ccgccatcct cgatgaagaa tttggtctgg aagagaaagg
     3781 ctacaccagt ctggtggttg ttccggtggg tcatcacagc gttgaagatt ttaacgctac
     3841 gctgccgaaa tctcgtctgc cgcaaaacat taccttaacc gaagtgtaat tctcccttgc
     3901 cgggcatctg cccggctatt tcccctcaga ttctcctgat ttgcataacc ctgtttcagc
     3961 cgtcatcata ggctgctgtt gtataaagga gacgttatgc aggatttaat atcccaggtt
     4021 gaagatttag cgggtattga gatcgatcac accacctcga tggtgatgat tttcggtatt
     4081 atttttctga ccgccgtcgt ggtgcatatt tttttgcact gggtggtact gcggaccttc
     4141 gaaaaacgcg ccatcgccag ttcacggctt tggttgcaaa tcattaccca gaataaactc
     4201 ttccaccgtt tagcttttac cctgcagggg attatcgtca atattcaggc ggtattctgg
     4261 ctgcaaaaag gcaccgaagc ggcagatatt ctgactacct gcgcgcagtt gtggatcatg
     4321 atgtatgcgc tgctttcagt cttctcgttg ctggatgtga ttttgaatct ggcgcagaaa
     4381 ttcccggcgg catctcagtt accgctgaaa gggatatttc aggggattaa actgatcggc
     4441 gcgattctgg tcggcatttt gatgatctcg ctgctgattg gtcagtcgcc agcgattctg
     4501 atcagcggtc ttggtgcaat ggctgccgtg ctgatgttgg tatttaaaga tccgattctt
     4561 ggtctggtgg caggtattca gctttccgcg aacgatatgc tgaaactggg cgactggctg
     4621 gagatgccga aatacggcgc ggatggcgcg gtgatcgata ttgggttaac caccgtcaaa
     4681 gtacgtaact gggacaatac cattaccact attcccacct ggtctctggt ttctgactcc
     4741 tttaaaaact ggagcgggat gtcagcatct ggcgggcgac gtattaagcg cagtatcagt
     4801 attgatgtca ccagtattcg ttttcttgat gaagacgaaa tgcaacgtct gaataaagcg
     4861 catttgttaa agccttattt aaccagccgc catcaggaaa ttaatgagtg gaatcgccag
     4921 caaggttcta cggagtcggt attaaacctg cgccgaatga ccaatattgg aacctttcgt
     4981 gcctatctga acgaatatct acgtaaccat ccgcggattc gtaaagatat gaccttaatg
     5041 gtacgccaac tggctcctgg cgataacggt ttaccgctcg agatctatgc gtttaccaac
     5101 accgtggtgt ggctggaata tgaaagcatt caggctgata tattcgacca catatttgcg
     5161 attgtcgaag agtttggtct gcgacttcat cagtcgccaa ctggcaatga tattcgctct
     5221 ctggcgggtg catttaagca gtaattaaaa aaaccgctct catcgaatgg atgagagcgg
     5281 tttcggatgg ttgacatcat tttgtcggat acggcgcgaa tgccttattt ccgacgcagc
     5341 gttttaaacg ctacaaacag gaatacaatc cacactggca gcagaatcgc tgacaagcgc
     5401 atatcatcca tcgtgcacat cagcagcaaa atcatgccga ggaaggcgat gcaaagatag
     5461 ttgccgaacg gatagagcag cgccttaaac tgtgtttcac gtccctgacg tcgcatcgcc
     5521 gcgcgaaaac gcagatgcgc caggcagatc ataatccagt tcaacagcag cgttgctacc
     5581 accagcgcca tcagcagacc aaacgctttt tgcggcagca gatagttaat cagcaccacc
     5641 agcgaagtga tggctccgga aagcatcagc gagttaatcg gcacgccgcg acggctgacg
     5701 cgagtcaaaa acttcggtgc attaccctgc acagaaaggc caaacagcat gcggctgtta
     5761 gagtaaaccc cgctgttata caccgacagc gatgccacca gaatgacgaa gttcagcgca
     5821 gaagctacca cgttgctgtc gagattatgg aaaatcatca caaacgggct actgttggat
     5881 ttcacttcca cccacggata gagtgccagt aaaaccacca gtgaaccgat ataaaacagc
     5941 agaatgcgat acaccacctg attcactgct ttcggaatgc ttttttccgg atcgcgcgct
     6001 tcagcggcag taatcccaat cagctccaga ccgccgaaag agaacataat taccgccagc
     6061 gacaaaatca gcccattcca gccggtggca aaaaaaccac cgtaacgcca gaggttatcg
     6121 atactggctt tctcgccgcc gtgaccagaa aacagcagcc acaggccaaa gccgatcata
     6181 ccgatgatcg ccagcacttt aatcagcgca aaccagaatt cggtttcgcc atataagcgc
     6241 acgttcacca ggttaacggc gttgatgata ataaagaagg cggcagccca aatccacgtt
     6301 ggaacatccg ggaaccagta ctgcatatag atgcccgcag cggtcagctc tgccattccc
     6361 accagcacga acattaccca gtagttccag ccagagagga agcccgcaaa cggtccccag
     6421 tatttatagg caaagtgggc aaatgaaccg gataccggct cctcaaccac catttcgcca
     6481 agctggcgca taatcaggaa agcgatgatc ccggcgacgc cgtagcccag caatacagcc
     6541 ggacccgcca tctgaatcgc cggaccaatg ccaagaaaca gaccagtacc aattgcgcca
     6601 cccagcgcaa tcagttgaat atgacggtta tgtaatccgc gatgaagcgt cggctcttga
     6661 ttcgacgcag tatcttccga tacggttgac gcgtttttca cgcctttccc ctgtgtgtct
     6721 tttttgttga ggggcacctt ttaacattta gtgcccatcg tcgcaagaca caatccacac
     6781 ggttaaacgg ggtatcctgc ttttatttcc gtacccgatg tcggtgcagc cacatcagct
     6841 tatacgccgc cgggataata aacagcgaca gcaaaggtgc ggtgatcatg ccgccaatca
     6901 tcggtgcggc aatccggctc atcacctctg aaccagcccc cgttccccac agaatcggca
     6961 gcagaccggc gataatcact gccaccgtca tcgctttcgg gcgcacgcgc aggaccgcgc
     7021 cgtgatacaa cgcctcatcc agcttctgct cgctgaatgt ttgcggatta ttcaacgacg
     7081 gctcggcctc tatggcgtga cgtaaataca tcagcatcac cacgccaaat tcggcggcaa
     7141 ccccggcaag ggcaataaaa ccggtgcccg tcgccacgga aagatgaaag cccatccacc
     7201 acagcaacca gatgccgccc accagcgcaa acggtacgct gctgataatc agcaacgctt
     7261 cgcccacccg acggaacgcc agatataaca gcacgaagat gatcatcagc gtcatcggca
     7321 ccatgagttt cagcttatgg ttggcgcgct ccagtagctc gaactgcccg gagaatgcca
     7381 cgctggtgcc cggttttaac tgcacttttt cagctatcgc tttttgcaga tcgtgaacca
     7441 ccgacaccat gtcacgatcg cgggcatcga tataaatcca gctcgtcggg cgcgcattct
     7501 cggttttcag catcgacggt ccggtagaga ctttaacgtc agccacgtct gccagggtga
     7561 tttgctgctt catcggcgtg aggatcggca actggcgcag tgcctgcggg ctatcgcgcc
     7621 agctttgcgg ataacgcaga ttaattggat aacgagcaat cccttccacc gtttcgccaa
     7681 ccatcgcccc gcccaccgca gaagtcacaa acaactgcac atccgccacc gtcataccgt
     7741 aacgcgcggc tttttcacgg ttaatctcaa cgttgatata acgcccacct tccagacgct
     7801 cagcaagcgc agaagccacg cctggcaccg ttcgcgccac ttcttcaatt tgctccgcca
     7861 tcgcgtcgat atccgccagc acggtgccgg aaactttaat accgatggga cttttaatgc
     7921 cagtcgagag catatcgata cggttacgaa ttggcggcac ccacagattc gccagccccg
     7981 gcaaacgcac ggtgttatcc agttcctcaa tgattttgtc catcgtcatg ccaggccgcc
     8041 actgatcctg cggcttaagc tgaatggtcg tttctaccat ctccagcgga gcagaatcgg
     8101 tggcggtttc cgctttcccg gtttggccaa atacccgcgc cacttccggt acgctcataa
     8161 tcagcttgtc ggttttttgc agcatactcg ccgcctccgc tgcggaaatc cccggcagcg
     8221 tcgatggcat atacaacaag tcgccttcat tgatctgcgg taaaaattcc ccgccaactt
     8281 tattgagcgg ccagagaacc gtcagcaccg aaagcgctgc caccaacagc gtggttttcg
     8341 gccagtgcag tactttcagc aacagcggat gataaacacg aatcaaaaag cgattgagcg
     8401 ggttactgct ttccggcgga attttgccac ggatccagta gcccatcagg atcgggatca
     8461 ctacgatcgc cagtagcgcc gcacccgcca tcgcataggt tttggtgaac gccagcgggc
     8521 caaacagacg cccttcctgc ccttccaggg tgaagatcgg gataaacgac agcgtgataa
     8581 tcagcagact gataaacagc gccggcccca cttcaacaga tgcatcggta atcacctgcc
     8641 agcgcgtttt attatccaac gtggcgtcag gatgctggtg ctgccactct tccagccgtt
     8701 tatgcgcatt ctcgatcatg acgatagcgg catcgaccat cgccccgacg gcaatcgcaa
     8761 tgccacccaa cgacataata ttggcgttca gtccctgaaa gtgcatgaca ataaaagcaa
     8821 tacacaaccc cagcggcaac gaaataatcg ccaccagcgc cgagcgcaca tgccagagaa
     8881 acagcgcaca gactaccgcc accacaataa actcttccag caacttgccg ctgaggttgt
     8941 cgatggcgcg atcaatgagc tggctgcgat cgtatgttgt aactatctcc acgccttccg
     9001 gcagactact tttcagcgtt tccagtttgt ccttcacggc ggcgattact tctcgggcgt
     9061 ttttgccgga gcgcaggatc accaccccgc ccgccacttc gccttcgccg ttcagttcgg
     9121 caatgccccg gcgcatctcc gggccaatct ggaccttcgc aacatcacgc agataaacgg
     9181 gcacgccatt ttcactggct tttaaaacga tgtgattaaa gtcgtcgagc gtttgcagat
     9241 agccgctggc tcgcaccata tattccgctt ccgccagttc gatcgacgaa ccacccgctt
     9301 cctggtttga agcatccagc gcgcttttta cttcggcgag actgatgcca tattgcgcca
     9361 ggcgctgagg atcgataacc acctgatact ctttcaccac accgcctacc gacgccactt
     9421 ccgcaacgtc aggaatggtt ttcagctcat atttgagaaa ccagtcctgc aatgagcgta
     9481 aatcggcaag atcgtgctta ccgctgcgat ccaccagtgc atattcatag atccagccaa
     9541 cacccgtggc atctggcccc agctcggcgc tgactcccgc aggcagctta ccctgtacct
     9601 ggttgaggta ctccagcacc cgcgagcgcg cccagtacgg atcggtgcca tcttcgaaaa
     9661 tgacatacac ataagagtcg ccaaactgcg agaaaccgcg cacagtcttc gcgccaggca
     9721 ccgacaacat ggtggtggtt agcggataag tcacctgatt ttcaacgatt tgcggtgcct
     9781 gaccgggata gctggtttta ataatcacct gcacatcaga gagatccggc agtgcatcca
     9841 ctggcgtgtt aatgatggtc caggtgcccc agatactcag aaacaacgcg cccatcagca
     9901 ccagaaaacg gttcgccacc gagcgacgaa taatccattc aatcattggt tattccctca
     9961 atgcgcatgg gtagcacttt cagagcgcat ccgctccagt gcgccagaaa tattggcttc
    10021 ggaatcaatc aggaacaggc cgctggaaac caccttttca ccttccgcca gaccagagcg
    10081 taacgcggtg acgccttgcg atgcctggaa aacggcaacg cgtttcggta caaagcgtcc
    10141 gtcggcatca acggtaatca cccgctgttc actgccggta tcaatcagcg cctgtgacgg
    10201 aatgagcagc atcggttcgc tggcggtatt gagttgcaac caggcgttca tgcccggttt
    10261 tagcgactcg tcggcgttgt cgacttccag acgcagctgc agcgtgcggg tcgcggcatc
    10321 cacgccgggt agcagggtcc atttgcgaat ggtgagtgtt ttatcaggtc gcgccggaac
    10381 ggtgagcgta aactgcgagg catctttcac cagccatgcg atggactccg ggatcgcggc
    10441 agtgatccac accgggtcca taccctgaat tttcgcgacc acgttatctt tggcgatatt
    10501 cattcccgcg cgcagatcaa acgcggtgat cacgccatca atgggtgctt tgagcgtaaa
    10561 gcgagtctgg attttttgcg tggcaatcag gcggcgaata tccgcctccg gcattcccgc
    10621 cagtcgcagt cgctcaagaa tgccttcagt ctgggtcgcc gtaccgccgg tttcgcgcag
    10681 cagtaaatac tcactctgcg cttccaccca gtcgggaatg gtcaggtcga gaagcggtgt
    10741 gcccttttgt actttatcgc ccacggtaag cggatacacc ttatcgataa acccggcagc
    10801 gcgagcctgc acaatggcat actgatactc gttgtaactg acattcgccg ggaaactctg
    10861 ggcaaaagtc agcggcccgc gcgttacggt cgccgttttc acccccagat tctgcgtctg
    10921 ggtcgggtca atgcgtacac cagacgcaga actctcttca tcggcatatt tcggcaccag
    10981 atccatatcc ataaacggcg atttacctgg tttatcgaac cgcgtattgg gatacatcgg
    11041 gtcgtaccag aataagattt tacgttctgc ggtcgacgtt ttttctgcgg gcggttccgc
    11101 ctttgcaaac caggtaaaac ctgccgcaga aataataccg cccgcgatca tgctgccgat
    11161 aataagcgcg atttttttca tctcattaaa cctgggttac tggctgactt taatatcctg
    11221 taataaagaa aggttgccct gctggacaaa attaaacgcc actttgtcgc cggttttaat
    11281 ttcactcatt ttcgtctgcg gggtgatggt aaagcgcatg gtcatctccg gccagttcac
    11341 cgcggtaatt ggatcgtggt ggatgatgat ttttttgctt tccagatcaa tggtttttac
    11401 cacaccggtg gcgctaatga cctgtggttg tgcctcgctc atggtttcat gatgatgttc
    11461 gttagcctgg gcattaaagc caataacggt aaacagacta aacattgcga cttgcagtgc
    11521 ttttttcatt tattctctcc tggagttaaa aattattgct gccaaccgcc gcctaaggcg
    11581 gtatataaag aaatttcatt aacctgacgg gcataattca ggtcaagtaa agtttgtcgg
    11641 gttgcaaata aagaacgctc agcatccagc acttccagat aacttaccgc gccgtgctga
    11701 tataatgccc gcgcccgttg caaagtaatt tgcagcgacg ccagataacg ctgctgggcg
    11761 ctaatttgat cgttcaggct ttgacgtaat gccagcgcat ctgccacttc tttaaaggcg
    11821 ttctggattt tctgttcata attcaccacc gactgctgct ggcgaatttc ggcgatatcc
    11881 agattggcct ggttacgtcc ggcattaaaa atggggatct caattttggg tataaaattc
    11941 cacatcccgc tgctggcgtt aaataatgac gacagatcgc tgctggcggt tgatattccg
    12001 ctggtcaggc tgatagacgg gaaaaatgcc gcgcgtgcag caccaatatt ggcattagcc
    12061 gccattaacg catgttcagc ttccataata tccgggcgct gcaataagat ttgcgacgac
    12121 aagcccgccg gtaatttaac gctttgcagg ctgtcgctgt ttactgtctg cgcttgcggc
    12181 agcttgccgt agcttcccaa taacagttgc aatgcattat tcgcctgcgc cagttccccc
    12241 tgacgtttag cgatgtcgct gcgggtactt tcaatcaccc cgcgagcctg ttccagcgcc
    12301 agaacattac tgctacctgt caacagttgt ttttcgacaa acgcatatga ctgctgataa
    12361 ttacgcagcg tttcttcggc tatttgcaat tgcgcatacg ccagctgctg attgaaatag
    12421 ctttgcgcga cattagaaac cagcaaaata tgcaccgcgc gctgagcttc ctcagtggct
    12481 aaataatttt ggcgctcggc ttcgctcatg ttctttaagc gcccgaaaaa atcgagatca
    12541 aagctggcgt taaggccagt cgagaactcc cgcgtcgtgg ctgaatcgcc tttaagattg
    12601 ttgctccagc tgccgctgcc ctcgccattg agctgtgggt agcggtcggc atcggtcaga
    12661 cgatattgcg cccgcgcttc ctgcactttc agcgtcgcca tgcgcaaatc ccggttattc
    12721 accagcgcct cgctaatcag cgtcttcacc tgattatcaa caaaaaaggt gcgccagccc
    12781 gcgttctggt agttatctgc tgcgttaacc aggccgttct ggttgagtga gaactgctgc
    12841 ggcacgggca ttgccgggcg ctgataatcc ggtgccagtg aacaaccggt tagcgcaagg
    12901 gccacacaaa atggcagaag tttacaagga gacataggct cataatttct ggtgatttta
    12961 tgccgccaac tttactcgcc aggctctgat tttccggtga caggaaaatg acaaaattgt
    13021 cattttgcca ataagcgatt gccatctgat cccgctactc tagaattgcc cgggcaacat
    13081 gcggaggaaa tatgaaactg ttgattgtcg aagatgaaaa gaaaaccgga gaatacttga
    13141 ccaaagggtt aaccgaagcc ggttttgtgg tcgatttggc cgacaacggg ctgaatggct
    13201 accatctggc gatgaccggt gattatgatc tgataatcct cgatattatg ctgccggacg
    13261 tgaacggctg ggatatcgtg cgcatgttgc gttccgccaa taaggggatg ccgattctgt
    13321 tgcttaccgc gcttggcacc attgaacatc gcgtcaaggg gctggagttg ggggcggatg
    13381 actatctggt gaagccgttc gcttttgctg aactgctggc gcgagtgcgc actctactgc
    13441 ggcgcggggc ggcggtgatt atcgaaagtc agtttcaggt tgccgatctg atggtcgatc
    13501 tcgtcagccg caaagtcacc cgcagtggca cgcgcatcac tctgaccagt aaagaattta
    13561 ctctgctgga gtttttcctt cgccatcagg gcgaagtact gccccgctca cttattgcct
    13621 cacaggtgtg ggacatgaat ttcgacagcg acactaacgc cattgatgtg gcggtgaagc
    13681 ggctgcgtgg caaaatcgac aacgactttg agccgaagct gattcaaacc gtgcgcggcg
    13741 tgggttacat gcttgaggtg ccggatggtc agtaagccgt ttcagcgccc gttttcgctg
    13801 gcaacccgcc tgaccttttt tattagcctg gccaccatcg cggcgttttt cgcctttgca
    13861 tggatcatga tccactcagt aaaagtgcat tttgccgagc aggatattaa tgatttaaaa
    13921 gagattagcg ccactcttga acgggtactt aatcaccctg acgaaacgca agcccaacgc
    13981 ttaatgacgc tggaagatat cgtcagtggt tattccaatg tgttgatttc cctggcagat
    14041 agccacggta aaacggtgta tcactccccc ggtgcgccgg atatccgcga gtttacgcgt
    14101 gacgccatac ccgataaaga cacgcggggc ggtgaggtgt atctcctttc cggcccgacg
    14161 atgatgatgc cgggccacgg tcacgggcat atggaacaca gcaactggcg gatgattagc
    14221 ttgccggttg gcccgttggt ggacggcaaa ccgatttata cgctctacat tgcgctttcg
    14281 atcgattttc atcttcatta cataaatgat ttgatgaata aacttattat gaccgcatcg
    14341 atcatcagca tcctgatcgt ctttatcgtg ctgttggcgg tacataaagg tcacgcgccg
    14401 atccgcagcg tcagccgtca aatccagaac attacctcga aagatctcga cgttcgcctt
    14461 gacccgcaga ccgtgcccat tgagctggaa cagctggtac tgtcgttcaa ccatatgatc
    14521 gagcgtattg aggatgtctt tacccgccag tccaatttct cagcggacat cgcccacgaa
    14581 attcgtacac caattacgaa tctcataacg caaacggaaa tcgccctcag ccagtcacgc
    14641 agccagaagg agctggaaga tgtgctctac tctaatctcg aagagctgac gcgaatggcg
    14701 aaaatggtca gcgatatgct gtttctcgcc caggccgata acaaccagtt aatccccgaa
    14761 aagaaaatgc tcaacctggc ggatgaagtc ggcaaagtgt tcgatttttt cgaggcatta
    14821 gcggaagatc gcggcgtgga gttgcggttt gttggtgacg agtgtcaggt tgcgggcgat
    14881 ccgctgatgc tgcgtcgggc gttaagcaac ctgctttcga acgccctgcg ttatacgcca
    14941 cccggagagg caattgttgt gcgctgccaa acggtcgatc accaggtgca agttaccgtc
    15001 gaaaaccccg gtacgcccat tgcgcccgag cacttaccgc gattgtttga ccgtttctat
    15061 cgcgttgacc cctcccgtca gcgaaaaggc gaaggtagcg gtattggcct ggcgatagtg
    15121 aaatcgattg ttgtcgcgca taaaggcacg gttgcagtaa cgtcagatgc tcgggggaca
    15181 aggtttatga tcaaactacc ggagaaaggt taattagtta gttcaaactc aaagagtgat
    15241 gtatcgcctt ctgttattat
//
LOCUS       DAZGQL010000113        15217 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.113, whole genome shotgun sequence.
ACCESSION   DAZGQL010000113 DAZGQL010000000
VERSION     DAZGQL010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15217
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.113"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(277..352)
                     /locus_tag="ACHKIR_004225"
     tRNA            complement(277..352)
                     /locus_tag="ACHKIR_004225"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(317..319),aa:Phe,seq:gaa)
     gene            complement(458..1165)
                     /gene="yqgA"
                     /locus_tag="ACHKIR_004226"
     CDS             complement(458..1165)
                     /gene="yqgA"
                     /locus_tag="ACHKIR_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFO9991886.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVTKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            1318..1395
                     /locus_tag="ACHKIR_004227"
     CDS             1318..1395
                     /locus_tag="ACHKIR_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9991887.1"
                     /translation="MHATSLHSLAYRRFARNSSLFLLMR"
     gene            1558..3693
                     /gene="speC"
                     /locus_tag="ACHKIR_004228"
     CDS             1558..3693
                     /gene="speC"
                     /locus_tag="ACHKIR_004228"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HFO9991888.1"
                     /translation="MKSMNIAASSELVSRLSTHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHVVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LAIIQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVALGIEARKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAAEQYFVDPCKLLLTTPGIDAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLFGYVLK"
     gene            complement(3743..4999)
                     /gene="nupG"
                     /locus_tag="ACHKIR_004229"
     CDS             complement(3743..4999)
                     /gene="nupG"
                     /locus_tag="ACHKIR_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HFO9991889.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(5201..6280)
                     /gene="mltC"
                     /locus_tag="ACHKIR_004230"
     CDS             complement(5201..6280)
                     /gene="mltC"
                     /locus_tag="ACHKIR_004230"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HFO9991890.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(6345..6620)
                     /gene="yggX"
                     /locus_tag="ACHKIR_004231"
     CDS             complement(6345..6620)
                     /gene="yggX"
                     /locus_tag="ACHKIR_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HFO9991891.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(6648..7700)
                     /gene="mutY"
                     /locus_tag="ACHKIR_004232"
     CDS             complement(6648..7700)
                     /gene="mutY"
                     /locus_tag="ACHKIR_004232"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFO9991892.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAATNNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            7861..8580
                     /gene="trmB"
                     /locus_tag="ACHKIR_004233"
     CDS             7861..8580
                     /gene="trmB"
                     /locus_tag="ACHKIR_004233"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFO9991893.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            8580..8906
                     /gene="yggL"
                     /locus_tag="ACHKIR_004234"
     CDS             8580..8906
                     /gene="yggL"
                     /locus_tag="ACHKIR_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HFO9991894.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLGEVRTSEL
                     FDVWWD"
     gene            9090..9809
                     /gene="yggN"
                     /locus_tag="ACHKIR_004235"
     CDS             9090..9809
                     /gene="yggN"
                     /locus_tag="ACHKIR_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HFO9991895.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            9985..11031
                     /gene="ansB"
                     /locus_tag="ACHKIR_004236"
     CDS             9985..11031
                     /gene="ansB"
                     /locus_tag="ACHKIR_004236"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HFO9991896.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDDVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDISKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKTVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            11148..12155
                     /gene="yggM"
                     /locus_tag="ACHKIR_004237"
     CDS             11148..12155
                     /gene="yggM"
                     /locus_tag="ACHKIR_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HFO9991897.1"
                     /translation="MKKQWIVGTALLMLMTGNVWADGEPPTENILKDQFKKQYHGILK
                     LDVITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPIKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     NQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREDAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSADCYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWITLEKGQYPLTMKVSEINSKKVAILMKIDDINQTNERWKKDTEQLRRNGVI
                     K"
     gene            complement(12211..13512)
                     /locus_tag="ACHKIR_004238"
     CDS             complement(12211..13512)
                     /locus_tag="ACHKIR_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFO9991898.1"
                     /translation="MDSYIALTLFGCFFVLVFIGVPISFSIGIATVASMLLMFPWDIA
                     AITVSQRLANGLDNFALLAIPFFIFAGTLMNSGGIAIRLINLAQVMVGRVPGSLGHVN
                     VLANMMFGSISGSAVAAAAAVGGTLNPIQTKEGYDPAFSTAVNVSSCITGLLIPPSNV
                     LIVFSLTAGGVSVASLFMAGYLPGILMGLAVMIVCGIIAKRRGYPLSERATFAQACKA
                     FLDALPSLLLVFIVMGGILGGIFTATEASAIAVVYTFILSVLIYREVKWRDLPKLILE
                     SVVTTSIVLLLIGFSVGMSWAMTNADIPYMISDALMGISDNPLVILLLINIVLLIVGI
                     FMDMTPAVLIFTPIFLPIAQELGMDPVHFGIMMVANLCIGLLTPPVGSALFVGCSISG
                     VKIQHLIKPLLPFYAALLIALMMITYIPQISLFIPQLLGLM"
     gene            complement(13512..14015)
                     /locus_tag="ACHKIR_004239"
     CDS             complement(13512..14015)
                     /locus_tag="ACHKIR_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFO9991899.1"
                     /translation="MIFNRLKLAVDRVIAAFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGAAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAG
                     SVIVTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGLVMMFYALCFINQSIQQLK
                     QPAQEVS"
     gene            complement(14060..15046)
                     /locus_tag="ACHKIR_004240"
                     /pseudo
     CDS             complement(14060..15046)
                     /locus_tag="ACHKIR_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
BASE COUNT         4032 a         3645 c         3857 g         3683 t
ORIGIN      
        1 cagacactgg aagatgccct gagtttgtat acagatgagc gcacagtgag caagatttgc
       61 aaagggggga ataaatcttg acaggctgaa tcagcaatgt aagcatacag tgttaacgag
      121 aacagggcgt ctctgcaaaa gacaccctgt ttgaggatac gataccgaac tcaatcagtg
      181 taaccacaac ttacacaaac ggagaccctt tgatgccgca tcctgtatta accgcccgtc
      241 aaggatctca tcccttaacc agcgtgttct ggtatatggt gcccggaggc ggaatcgaac
      301 caccgacacg gggattttca atcccctgct ctaccgactg agctatccgg gcaacggggc
      361 gcattaaacc tgattcgcct cgtctcgtca atcaaatttc ttcaatttac tgcagactgc
      421 acaatctatc atcactttgc cgctattgca cgcattgtca ggcaaaccag gcggtccagg
      481 cggcggaaag cggcattgcc agaaggagtg ctggaagcat gttgaccacc gggaacattt
      541 taatgccaca gatgcgtaat ccggttgcca gcagcaataa accgcctacg gcgctgaagt
      601 ctgccatcat cgacggtgtg gtcagcggta atatcagcgc ggcagcccac gccagcgtta
      661 actggatgat cagtaatggg atactaatta ccgacaccgc aatacccaac gagcaggcga
      721 agatcatcgc cgtaaagaaa tcgagaaatg acttggcgat taaaatactc ggatcgccgg
      781 tcatcccttc gttcatcgcc ccgaagatcc cggtgccgct ggcgcaaaac aggacaataa
      841 tcgcgacata attctgaata aaagattcat gcgctggctt cttacgtgag tggcgaaaca
      901 gattttgtgc tttggtgacc gcggtattga cgcctttttc cagcagacaa atttcgccga
      961 ttaatgcacc gagtaaggtt gctaaaacca tcgccggaag gttggcgcat ttcaccacca
     1021 gtaaaatacc aatccccagc gatgccagac caaaaataga tgtcatggag acgcggatac
     1081 gttccggtaa acgttggctg agcagtgcgc cgagaacgcc acccagtaag actgcactag
     1141 cgttgataaa agggccgatg accacaagag ttcctgttaa ctgtattgtc tgattttctt
     1201 attatgaact ttttgccttg ttgatggctt gtctggtcgc aaagtcctgg cttgcacgct
     1261 ttagcgaaag gtgccatgat tgcgcgaatt ttctcctctc tgtacggagt ttgcccgatg
     1321 cacgccacct ccttacattc tctcgcttat cgccgtttcg cgcgaaactc ctcccttttt
     1381 ctgctcatgc ggtgaagtta acgcacgctc actgcaggac aacagtaaaa tcagagcgtt
     1441 tctgctttta ctgatgtctg gcggtcggag ctggtgacca gtttgaccca catctcatgg
     1501 ggcaggtttg tccatcctgt ccggtactct tacttccccg aaacgggttt tgcgcttatg
     1561 aaatcaatga atattgccgc cagtagtgaa ctggtatccc gactttctac tcatcgccgc
     1621 gtggtggcgt tgggagatac tgattttacg gacgtcgcgg cagtcgtcat taccgctgcg
     1681 gatagtcgca gtggcattct tgcgttgctt aagcgcaccg gttttcatct accggtgttt
     1741 ttgtattccg aacatgttgt tgaattacct gcgggcgtta cggcggtaat caacggcaac
     1801 gagcagcagt ggctggagct ggaatccgca gcctgtcagt atgaagagaa tttgctgcca
     1861 ccgttttatg acacgctgac gcagtacgtt gagatgggca acagcacctt tgcttgccct
     1921 ggacatcaac atggtgcgtt ttttaaaaag catcctgccg gacgccattt ttacgatttc
     1981 tttggtgaga acgtctttcg cgccgatatg tgtaacgctg acgtaaaatt gggcgatctg
     2041 cttattcatg aaggttcggc gaaggatgca cagaaattcg cagccaaagt ctttcatgcc
     2101 gataaaacct attttgtgct gaacggcaca tcggcagcga ataaagtggt gacgaatgcg
     2161 ctgttaacgc gtggcgatct ggtgctcttc gaccgtaaca accataagtc gaatcatcat
     2221 ggcgcgttga ttcaggcggg ggcgacgccg gtctatctgg aagcttcacg caacccgttt
     2281 ggtttcattg gtggtattga tgcgcactgt tttaatgaag agtatctgcg ccagcaaatt
     2341 cgcgacgttg cgccagaaaa agcagacctg ccgcgtccgt ttcgtctggc gattattcag
     2401 ctgggaacct atgacggcac tgtctataac gcccgtcagg tgatcgatac cgttgggcat
     2461 ctgtgtgatt acattctgtt tgattccgcg tgggtcggtt acgaacagtt tatcccgatg
     2521 atggcggata gctcgccgct gctgttagaa cttaacgaaa acgatccggg gatctttgtg
     2581 acgcaatcgg tgcataaaca gcaggcggga ttctcacaga cgtcgcagat ccataaaaaa
     2641 gataaccata ttcgcgggca ggcgcgtttt tgcccgcata agcggttaaa taatgccttt
     2701 atgctccatg cttctaccag cccgttctat ccgctgtttg ccgcgctgga cgttaacgcc
     2761 aaaattcatg aaggggagag tgggcgtcgg ctgtgggcgg agtgcgttgc gttggggatt
     2821 gaagcccgga aggcgattct tgcgcgctgt aagctgttcc gcccgtttat tccacccgtt
     2881 gttgatggca aattgtggca ggattatccg acgtcagtgt tagccagcga ccgtcgtttt
     2941 ttcagttttg agccgggggc gaagtggcac ggctttgaag gatatgccgc ggagcagtat
     3001 tttgtcgatc cgtgcaagct gttactgacc acaccaggta ttgatgctga aaccggtgaa
     3061 tatagcgact ttggcgttcc ggcgacgatt ctggcgcatt atctgcgtga gaacggtatt
     3121 gtgccggaga agtgcgatct caactccatt ctgttcttat taacgccggc ggaaagccac
     3181 gagaagctgg cacaactggt ggcgatgctg gcgcagtttg aacagcatat tgaggatgac
     3241 tcgccgctgg cggaggtgtt gccgagcgtt tataacaaat atccggtgcg ctatcgcgac
     3301 tacactttgc gccagttgtg tcaggagatg cacgatctgt atgtcagttt cgacgtcaaa
     3361 gacctacaaa aagcgatgtt ccgccagcag agtttcccgt cagtggtgat gaacccccag
     3421 gatgcgcata gcgcttatat tcgcggtgaa gtggagttgg tgcggattcg tgatgccgaa
     3481 gggcgaattg cggcagaagg ggcgttgcct tatccaccag gcgtgctttg cgtggtaccc
     3541 ggagaagtct ggggtggggc ggttcagcgt tatttccttg cgctggaaga aggggtgaat
     3601 ttattgccgg gattttcgcc ggagctgcaa ggtgtttata gcgaaaccga tgcggatggc
     3661 atgaaacggt tgttcggtta tgtgttgaag taagaataaa aaaaacgggt caccttctgg
     3721 cgacccgttt ttctttgcgt aattagtggc taaccgtctg tgtgcctgtc gggacacgaa
     3781 cgtgtttata tttgaacatc gccatgaacg cgaaggccag aaccacggag taaccagcga
     3841 aaatcagcca tacggtctgc cagtcggtaa tgccgttttg ggtgtacatc tcaacaactt
     3901 taccgctcac gatgccgccg aggatacagc cgaagccgtt agtcatcatc aggaacatcc
     3961 cctgcgcact ggcgcgaatt gccgggctaa cttctttttc gacaaacacc gaaccagaga
     4021 tgttgaagaa gtcgaatgcg caaccgtaaa cgatcatcga cagtaccagc agtacagtac
     4081 cgaacggagt cgggtcgccg taagcaaaca gcgcaaaacg caggatccac gccacaatac
     4141 tgatcatcat tacgttctta ataccgtagc ggcttaagaa gaacgggatg gtcagaatga
     4201 acagggtttc agagatctgc gaaatcgaca tgatgattga cgcatgctgc acgatgaagc
     4261 tgctggcaaa catcggatct ttgtcgaagc tgtgcaggaa ggtgttaccg aacatgttgg
     4321 taatctgcag ttccgcgccc agcagcattg agaagatgaa gaagattgcc atacgcttgt
     4381 ttttaaacag cgcgaatgca tcgaggccca gcagggttgt ccagctctga ttcgcttgct
     4441 gtttagcaac cggaatatgc ggcagagtca gggtaaacag aaccagaatg gcggaaagtg
     4501 ctgcgccaat atacagctgc atgtggctta attcgaagcc agacaggctc accacccaca
     4561 ttgccatgat aaagccgatg gtgccccaga tacggattgg cgggaagtca gtaacgatat
     4621 ccatcccggc attttgcagg cgatagtaag agatggtgtt gattaaccca agcgttggca
     4681 tataagcaaa cgagttaatc aatatcacaa ggaacatcgc ttccggcgtc gtgacctgtg
     4741 ccgccatgaa cagcgtgata gcgccaatgg tgtggcaaat ggcatatacc catttcgcac
     4801 ttaaccattt gtcggccaca atccccagca gcgcaggcat aaataccgcg gcgataccca
     4861 gtgagctata aactgcgcca atagaagcac cgtcaaactt cagggtaaca aacatatagg
     4921 agccgagggt cgtcagccaa cttccccaca gacagaactg cagaaaagag aggattttca
     4981 gctgcagctt aagattcatg ttaatttcct cacatcgtga tgcggatgag tgtttgaaaa
     5041 agcacatttg accgtgcgag atgatgggat tctatcaact tcccggactg gttttttgtt
     5101 acctgtggca aataattgca aacattccca atttgcaaaa cgaaattgtg atgtggataa
     5161 catttttacc cctgagcatc gtcaggggcg gttagtggaa ttatcggcgg cggtaggatt
     5221 tttgcgcggt attcacttta taaagataac ggcgagattc cgcagaggga tggcgggtcg
     5281 tcagcgtttg ataaacatcg cccggcgtca tggtgttaat aatattggcc gcctgaatct
     5341 tatcattcga aaagactcgc agcacgctgc ctgcgccgcc gttataggcg gtgatgacgg
     5401 cataacgccg ggatgtaggg ttatcaattc cgccgagata aacgttgttc agcatcgcca
     5461 gatacgcggt gccggtatca atattgctgg caggatcaaa caagaaactg cggctcggcg
     5521 tgccggattt cccctgcgaa cggaacacat ctttcccggc agtatgttgt accacctgca
     5581 ttaaccccag cgcatcggaa cggctgaccg catacgggtt aaaggatgac tcggtctgca
     5641 taatcgccag aatcagcgac tcatcaacgc catatttccg tgacgcctgg cggaccatgc
     5701 cgagatattt gtgcgcacgt ttatcaaggt ggttcggcac catgttaatg gtgacgctgt
     5761 agatgatacg cagcccgttg ctgcggctct tcagacggtt tttcagcaga taatccgcga
     5821 agttgctcgc gcgaccttcc cagcgaatcg gctgcccggt gttgtccacc acctgaccgt
     5881 aaaggaaagg ttctttcgaa atcgtaatat catcaacgtc ggaatagaga tcgaccgaac
     5941 tcggatcgtc ccccatcagc aacgttttga taattgcccg gcgcagatgc gcggcaggtt
     6001 ctgtcccggc gatggtttcg atagtaatcg taccgtcatc gaagttgatg tggctgcggg
     6061 tctgatattg atcggtgtat ttcacgtagt ccttaggacc ggcgatcacc acctctttga
     6121 agccccagat gttctcaata ttgtgggcaa attgccccat cagaatatca aaaccgttgg
     6181 tatctttgac ccaggcttcg ttataggtat cgcctttttt ggtcgtcgaa caggagatga
     6241 gcaacggcgc aatcaaagcc agcgcgagat attttttcat cattccgggt gcgtgttgtg
     6301 tttgttttta tgcaagttgc cggaggcgtg ctccggcact gtttttattt tttatcttcc
     6361 ggcgtatagc cctcaatatg cacctcttta ccctcgaaca ggaagttgac catctcctgt
     6421 tccagcagct tgcggtgctc ggcattcatc atgttgagtt tcttttcatt aatcagcatg
     6481 gtttgtttgt gctgccactg cgcccaggct tctttggaga tctcgttata gatgcgtttt
     6541 cccagctcgc cggggtacag ctgaaaatcc tgaccttctg cttcacgttg caggaaagta
     6601 caaaaaatcg ttctgctcat aaatcatcct ctttatcgac tcacgcgcta aaccggcgcg
     6661 ccagtgcgta actgctgtaa caaacgctcc acgggagccg ccaggccaac tgacggcggt
     6721 tgcgctaagt tataccagag cgcattgcct tcatccatgc agccggtgaa tgacgacacg
     6781 ggaagccaca taggcacaat atctaagtgg aaatggctga aggtatgccg aaacgcggtc
     6841 agctgcgtca ggttatcggc agcaatctgc cgttgcgcca gccactgccg caaactttct
     6901 tcgtcggcaa actgcgggaa acagtataaa ccgccccaca atccgctcgg cggacgctgc
     6961 gccagcaata cttcatcttc gtgctgtaac aggagaaagt agccggtgcg ctccggcagc
     7021 gtctgtttcg gttttttgcc cggataaagc gaccagctat tgttggtggc ggcaatacat
     7081 ccgttttgta gcggacagag cgagcatttc ggcttcgagc gcgtgcaaat catcgcaccc
     7141 aaatccatca tcgcctgatt aaaccgctcc acgccgaccg cgggcgtcac ctgttcgctc
     7201 aaactccaca gtttattctc gacctctttt ttcccaggcc agccgcttac agcatagcag
     7261 cgcgccagca cccgtttgac gttaccgtcg agaatcggaa agtgcttacc cagagaaagc
     7321 gagagaattg cgcctgcggt ggaacgcccg acgcccggta acgccgcgac ttcttcaaag
     7381 gtttccggga atttaccgcc gtgtaaggtc gccacttgtt gtgccgcctt atgcagattg
     7441 cgcgcgcggg cgtaatagcc aagcccggtc cacaagtgga gaacttcgtc gagcggcgca
     7501 ttggcgagat cggtcaccgt cgggaagcgc gccataaagc gttcaaaata ggggataacg
     7561 gtcgcaacct gagtttgttg caacatcact tctgagagcc atactttgta gggcgtcttg
     7621 tcaatttgcc agggcagcgt tttccgcccg tatttatcgt accagtccag aacctgggct
     7681 gaaaattgcg acgcttgcat ggtcaccgaa ttcactgttg ttgggggcaa gattgcagca
     7741 cagcgacggc agggtgtaaa ccggaacttt ccgcagccac ggccttatca tgcttgcatc
     7801 cggcaactaa ctttggataa tgcccgtttt cagaacactt tcacaagcga ctaaatcttt
     7861 atgaaaaacg acgtcatctc accggaattt gatgaaaacg gccgcccact gcgccgtatc
     7921 cgtagttttg tgcgccgcca ggggcgactg accaaaggcc aggaacatgc gctggaaaac
     7981 tactggccgg tgatgggcgt tgagttcagc gaagatatgc tggatttccc cgcgcttttt
     8041 ggccgagaag cgccggtgac gcttgagatt ggtttcggca tgggggcgtc gctggtggca
     8101 atggctaaag atcgccccga gcaggacttc ctcggcattg aagtgcattc accgggcgtt
     8161 ggtgcgtgcc tggcttctgc gcatgaagag ggcttaagca acctgcgcgt gatgtgtcac
     8221 gatgcggttg aagtgctgca taaaatgatt cctgacaatt cattgcgcat ggtgcagctc
     8281 tttttccctg acccgtggca caaagcgcgc cataataaac gccgtatcgt tcaggtgccg
     8341 tttgccgaac tggtaaaaag caaactgcaa ctggggggcg tattccatat ggcgaccgac
     8401 tgggaacctt atgcggaaca tatgcttgaa gtgatgtctt ctattgacgg ttataaaaac
     8461 ctgtcagaga gcaatgatta cgtaccgcgt ccggcatcac gtccggtgac gaaatttgaa
     8521 caacgtggtc atcgtcttgg tcacggagta tgggacttaa tgttcgagag ggtgaaataa
     8581 tggcaaagaa ccgtagccgt cgtctgcgta aaaaaatgca catcgacgaa ttccaggaat
     8641 taggattttc ggtggcatgg cgattcccgg aaggtacatc ggaagaacag attgataaaa
     8701 ccgttgatga ctttattaac gaggttatcg aaccgaacaa actggccttt gacggcagcg
     8761 gttatctggc ctgggaaggt ctgatctgca tgcaggaaat cggcaaatgc accgaagaac
     8821 atcaggcgat tgtgcgtaag tggctggaag agcgcaaact gggcgaggta cgcaccagcg
     8881 aacttttcga cgtttggtgg gactaagaaa gcatacgggc gatgacaaat gcaaaactgc
     8941 ctgatgtgct acgcttatca ggcctataaa gatgcacgat cgagtaggct ggataaggcg
     9001 tttacgccgc atccggcatg gaaaacgcgc actttgttat caatctgggg ccagcaaatg
     9061 ctggcctgat ttgttcttga gggaagacta tgatgcgcaa aatgctgctg gcggcagcac
     9121 tttcagtgac ggcaatgacc gctcacgccg actaccagtg cagcgtcacg ccgcgtgacg
     9181 atgtgattgt cagcccgcaa accgtgcagg tgaagggcga aaacggcaat ctggtgatca
     9241 cgccagacgg caacgtgatg tataacggta agcaatattc cctgaatgcc gcccagcgcg
     9301 agcaggcgaa ggattatcag gctgaactcc gtagcaccct gccgtggatt gatgaaggcg
     9361 cgaaaagccg cgtcgaaaaa gcccgtattg cgctggataa aattatcgtt caggagatgg
     9421 gcgaaagcag caaaatgcgc agccgtctga ccaaacttga tgcgcagctg aaagagcaga
     9481 tgaaccgcat tatcgaaacg cgcagcgatg gcctgacgtt tcactataaa gccattgatc
     9541 aggttcgcgc cgaaggccag caattagtga atcaggcaat gggtggaatt ttacaggaca
     9601 gcattaatga aatgggcgcg aaagcggtgc tgaaaagcgg cggtaaccca ttacagaatg
     9661 tgctgggaag cctgggcggt ctgcaatcct caatccaaac cgagtggaaa aagcaggaaa
     9721 aagatttcca gcagtttggc aaagatgttt gtagccgcgt tgtgactctg gaagatagcc
     9781 gcaaagccct ggtcgggaat ttaaaataat cctctatttt aagacggcat aatacttttt
     9841 tatgccgttt aattcttcgt tttgttacct gcctctaact ttgtagatct ccaaaatata
     9901 ttcacgttgt aaattgttta acgtcaaatt tcccatacaa agctaaggga taatgcgtag
     9961 cgttcacgta actggaggaa tgaaatggag tttttcaaaa agacggcact tgccgcactg
    10021 gttatgggtt ttagtggtgc agcattggca ttacccaata tcaccatttt agcaactggc
    10081 gggaccattg ccggtggtgg tgactccgca accaaatcta actacacagc gggtaaagtt
    10141 ggcgtagaaa atctggttaa tgcggtgccg caactgaagg acattgcgaa cgttaaaggc
    10201 gagcaggtag tgaatattgg ctcccaggac atgaacgatg atgtctggct gacactggcg
    10261 aaaaaaatta acaccgactg cgataaaacc gacggcttcg tcattaccca cggtaccgac
    10321 acgatggaag aaaccgctta cttcctcgac ctgacggtga aatgcgacaa accggtggtg
    10381 atggtcggcg caatgcgccc gtccacgtcc atgagcgcag acggtccatt caacctgtat
    10441 aacgcggtag tgaccgcagc tgataaagcc tccgctaatc gtggcgtgct ggtggtaatg
    10501 aacgacaccg tactggacgg tcgcgatgtc accaaaacca ataccaccga cgtagcgacc
    10561 ttcaagtctg ttaactacgg tcctctggga tacattcaca acggtaagat tgactaccaa
    10621 cgtaccccgg cacgtaagca caccagcgat acgccgttcg atatctctaa gctgaatgag
    10681 ctgccgaaag tcggcatcgt ttataactac gctaacgcat ccgatcttcc ggctaaagca
    10741 ctggtagatg cgggctatga tggcattgtt agcgctggtg tgggtaacgg caacctgtat
    10801 aaaaccgtat ttgacactct ggcgaccgcc gcgaaaaacg gcactgcagt cgtgcgttct
    10861 tcccgcgtac cgacgggcgc taccactcag gatgccgaag tggatgatgc gaaatatggc
    10921 ttcgtggcct ctggcacgct gaacccgcaa aaagcgcgcg ttctgctgca actggctctg
    10981 acgcaaacca aagatccgca gcagatccag cagatcttca atcagtacta atcacctcgc
    11041 cccggtaacg tgccggggct ttttcacttc agacttacgt ccattgccaa ttttcattac
    11101 cctgatgata atcactggaa taaattattc cgcgcgaggg ttttcgggtg aaaaagcaat
    11161 ggattgttgg cacggcgctg cttatgttga tgactggtaa tgtctgggca gatggcgaac
    11221 cgccaactga aaatatctta aaagatcaat tcaaaaagca gtatcacggc attctcaagc
    11281 ttgatgtcat caccttaaaa aatcttgatg ctaagggtaa tcaggccacc tggtcagcgg
    11341 aaggcgatgt ctcttccagt gacgatctct atacctgggt cggtcagttg gcagattacg
    11401 aactgctcga acagacctgg acgaaagata aaccgataaa attctcggcg atgttaacca
    11461 gtaaaggaac gcccgcgtct ggctggtcgg tgaactttta ctcttttcag gcggcagcca
    11521 gcgatcgtgg acgggtggtc gacgatatca aaacgaataa taaatatctg atcgtgaata
    11581 gcgaagattt caattatcgc tttagtcagc ttgaatcagc gttgaataac cagaagaact
    11641 cgattcctgc gctggaaaag gaagtgaaag cgcttgataa gcaaatggtg gcggcgcaga
    11701 aagcagcgga tgcatattgg ggaaaagatg caaacggtaa gcaaatgacc cgcgaagacg
    11761 cctttaaaaa aattcaccaa cagcgtgatg agtttaataa acagaacgat agcgaggcgt
    11821 tcgcggttaa atatgacaaa gaggtttatc aaccggcgat tgcggcatgt cataaacaga
    11881 gtgcagattg ttatgaagtg ccgattcagc agaagcgaga tttcgatatc aacgaacaac
    11941 ggcgacagac ttttctgcaa tcacaaaaac ttagccgtaa attgcaggat gactggataa
    12001 cgctcgaaaa agggcaatat ccgttaacga tgaaagtttc agaaataaac agtaaaaaag
    12061 tcgcgatttt gatgaaaatt gatgatatca accagactaa tgaacgctgg aaaaaagata
    12121 ccgaacaatt gcggcgcaat ggagtgatta agtaactcat gttaagtacc tttgccggat
    12181 aatataaaaa gttatccggc aaattatttt ttacattagc cctagtagct gtggaatgaa
    12241 caaggatatt tgcgggatgt aagtgatcat catcaacgcg attaacaatg cggcataaaa
    12301 tggcagcaat ggtttgatca aatgttgaat cttcacacct gaaatagaac aaccaacaaa
    12361 tagcgcacta cccaccggcg gtgttaacag cccaatacaa aggttagcca ccatcataat
    12421 accgaaatgt accggatcca ttcccagctc ttgtgcaatc ggcaagaaga ttggtgtgaa
    12481 gattagtaca gccggtgtca tgtccataaa gatgccgaca attaataaga cgatattaat
    12541 cagcagcaat atgaccagtg ggttatcaga aatccccatc aacgcgtcgc tgatcatata
    12601 aggaatatca gcattggtca ttgcccagga catgcccacg gaaaaaccga tcaacagtaa
    12661 tacaatcgaa gtggtaacaa ccgattccag aatcagtttg ggtaaatcgc gccattttac
    12721 ttcccggtaa atcagcaccg agagaataaa tgtgtagaca acggcaattg ctgacgcttc
    12781 agttgcggta aaaatgccgc ccagaatgcc gcccattaca atgaacacca gaagcaaact
    12841 tggtaaggca tccagaaacg ctttacatgc ctgagcaaag gttgctcttt ctgaaagtgg
    12901 gtacccacgg cgtttagcaa taatgccgca gactatcatt accgcgagac ccattaagat
    12961 ccctggcaaa tatcccgcca taaatagcga tgctacagaa acgccgccag ctgtcaggga
    13021 aaacacaatt aacacgtttg agggcggtat cagtaatcca gtaatacaag atgagacgtt
    13081 tacagcggta gaaaatgccg gatcataacc ttctttggtc tgaatgggat tcagtgtgcc
    13141 accaacggca gcagctgccg caacggctga accagaaatg gaaccaaaca tcatattcgc
    13201 caggacgttc acatgaccaa gcgaaccagg aacgcggcca accattacct gggcgaggtt
    13261 aatcaaacgt atcgcaatac cgccgctgtt catcagcgtt ccggcaaaga tgaaaaaggg
    13321 gattgccaat agggcaaagt tatccagtcc attagccagt cgctgcgaaa cagtgattgc
    13381 cgcgatatcc cagggaaaca tcaacagcat tgaagctaca gtggcaatac cgattgagaa
    13441 cgaaatgggc acaccaataa agaccagcac aaaaaaacag ccaaacagtg taagcgcgat
    13501 ataactatcc attaactgac ctcctgcgca ggttgtttca gttgttgaat gctttgatta
    13561 ataaaacaca aggcataaaa catcatcacc aatccactta atggcagaat gatatatacg
    13621 tatcccatcg gtatttgcat cgcggcagat acctgtgatg ttgccagtgt tttatcaatt
    13681 aatttcaggc caccagtcac aatgaccgaa ccggcaaaac ctaaaataag aacgttaatg
    13741 actatactaa gaagtaattg cttgcgctta ttcagggcga gagcaaataa atcaatagag
    13801 agatgtctct gcgcgccgac ggtatatgca gcacctaata atcctaccca gatcatcaaa
    13861 aagcgtgcca gctcatcggt taatgtactt ggctggttaa gtacgtagcg gctaaatact
    13921 tgccagacta cgcatacaac cagagcaagc attacggcga cagaaaacgc ggcaatgacg
    13981 cggtctaccg caagtttcag gcggttaaat atcattgtaa gcatcctgga aaatcagggc
    14041 cagagactgg cccgaaatat tattgagcag cgttgtcgaa tttctctaac aaagcagctt
    14101 gttttggatc ttttttgaag tcgtcaaaca gaggctgaac tgcggcacgg aatggggctt
    14161 tatcaacctt aacgatttca ccacccatgg ctttcgcctg agcacgtgtg tcagcgtcaa
    14221 ttttttccca caatttttgt tgataagctt cggatttctt cgccgctgtt tcgaggattt
    14281 gttgctgttc tggggttaat ttattccatg ttttggtgga gataaccaaa aagtcaggaa
    14341 ttgaggcatg ttcatcttca gaaaagactt tggcaatttc gatatgtcga gtctgcaccc
    14401 aggaaggcac gttattttct gcgccatcta ctacaccttg ctgcatcgca gtataaactt
    14461 cgccaaatga aattggagtg ggtgagccac ccatcagttc aatcattttg atggtggtcg
    14521 ggcttggctg aacacgaatt ttcagccctt taagatcttc tggcttagta atcggttttt
    14581 tcgcatagaa actgcgtgta cccgctacat aagctgataa agcaaaaaag cctttatctt
    14641 ttgttgaatc cataatttct ttaccaactt caccaaaaac cactttgttg aagtgcgcct
    14701 gatctttaaa taagaacggc aaattataga tagcataaat attatcaaaa gactccagat
    14761 cactggcgga acctttggtc atatccagtg caccattttg caacaactac atagtttctc
    14821 gcgcattacc catctggctg ctggggtaaa tacggatcac catattacct tttgacagct
    14881 gttttacttc tttcgccagc tcttcaaaac tctgatgtac aacatgactt ctttccaggt
    14941 tgtgtgctaa ctttaacgtc actttttctg cggcgtttac gccagtgcta accagagcca
    15001 gcaaggatgc gcagactaat gagcgggtaa atgacctcat tttcataaaa aaactccctg
    15061 ttgaagaatg gaaattggtt gatatctaat tgtgcttatc agtgctttgt gcgacaaatt
    15121 ggttaacctg tctgacaact tgtctatgtt tatcacaact tgaaataaag tcagaaatta
    15181 aatattaatt ttgtgcgcca ggttgtacat gtttata
//
LOCUS       DAZGQL010000114        15112 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.114, whole genome shotgun sequence.
ACCESSION   DAZGQL010000114 DAZGQL010000000
VERSION     DAZGQL010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15112
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.114"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..144)
                     /locus_tag="ACHKIR_004241"
     CDS             complement(<1..144)
                     /locus_tag="ACHKIR_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9991900.1"
                     /translation="MLQSSADKACNVLVKKVRYLNDVRKITKMMNGKLIYSWLVWPCS
                     EFFS"
     gene            222..1316
                     /gene="ddlA"
                     /locus_tag="ACHKIR_004242"
     CDS             222..1316
                     /gene="ddlA"
                     /locus_tag="ACHKIR_004242"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFO9991901.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(1519..1731)
                     /gene="yaiZ"
                     /locus_tag="ACHKIR_004243"
     CDS             complement(1519..1731)
                     /gene="yaiZ"
                     /locus_tag="ACHKIR_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HFO9991902.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            1991..2299
                     /gene="yaiY"
                     /locus_tag="ACHKIR_004244"
     CDS             1991..2299
                     /gene="yaiY"
                     /locus_tag="ACHKIR_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HFO9991903.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(2358..3452)
                     /gene="yaiW"
                     /locus_tag="ACHKIR_004245"
     CDS             complement(2358..3452)
                     /gene="yaiW"
                     /locus_tag="ACHKIR_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HFO9991904.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(3465..4685)
                     /gene="sbmA"
                     /locus_tag="ACHKIR_004246"
                     /pseudo
     CDS             complement(3465..4685)
                     /gene="sbmA"
                     /locus_tag="ACHKIR_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308454.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
     gene            5037..6194
                     /gene="ampH"
                     /locus_tag="ACHKIR_004247"
                     /pseudo
     CDS             5037..6194
                     /gene="ampH"
                     /locus_tag="ACHKIR_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
     gene            complement(6195..6818)
                     /gene="iprA"
                     /locus_tag="ACHKIR_004248"
     CDS             complement(6195..6818)
                     /gene="iprA"
                     /locus_tag="ACHKIR_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HFO9991905.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNSEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGSCAGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(6904..9852)
                     /gene="ehaB"
                     /locus_tag="ACHKIR_004249"
     CDS             complement(6904..9852)
                     /gene="ehaB"
                     /locus_tag="ACHKIR_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin EhaB"
                     /protein_id="HFO9991906.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAATNDISGQTYNTFHHYN
                     DATYADDVYYDGYVGWNNYAADSYYNGDIYPVINNATVNGVISTYYLDDGISTNTNAN
                     SLTIKNSTIHGMIYSECMTTDCADRADDYYHDRLALTVDNSTIDDNYEHYTYNGTYNN
                     AADTHVVNVFNIGTAITLDQEVDLSISNNSHVAGITLTQGYEWEDIDDNTVSTGVNSS
                     EVFNNTITVKDSTVTSGSWADEGTTGWFGNTGNASDYNGDGWNADDVALAVIAHPYAD
                     NAMQTTATFDNSTLMGDVFFSSNFDENFFPQGRDSYRDADGDVDTNGWDGTDRLDLTL
                     NNGSKWVGAAMSAHQIGVDTDDDGVNDSYTYGINTEATATLIDIAANSLWPSSTVGVE
                     NSDSEYSEFDHIIGNEVYQSGLFNVTLNTGSQWDTTKTSLIDTLSINSGSTVNVADST
                     LISDSISLTGLSALNINEDGHVATDSLTVDNSTVTISDEVSAGWAVGDAALYANNIKV
                     TNDGILDVGNTAANALQVDTLNLTSTTDTSGNIHAGVFNIESNRFVLDADLTNDRTND
                     TTKSNYGYGLIAMNSDGHLTINGNGDNDNTASIEAGQNEVDNNGDHVAAATGNYKVRI
                     DNATGAGSIADYNGNELIYVNDKNSNATFSAANKADLGAYTYQAEQRGNTVVLQQMEL
                     TDYANMALSIPSANTNIWNLEQDTVGTRLTNSRHGLADNGGAWVSYFGGNFNGDNGTI
                     NYDQDVNGIMVGVDTKIDGNNAKWIVGAAAGFAKGDMNDRSGQVDQDSQTAYIYSSAH
                     FANNVFVDGSLSYSHFNNDLSATMSNGTYVDGSTNSDAWGFGLKAGYDFKLGDAGYVT
                     PYGSVSGLFQSGDDYQLSNDMKVDGQSYDSMRYELGVDAGYTFTYSEDQALTPYFKLA
                     YVYDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDVDQDW
                     SANVGVKYTW"
     gene            10378..11352
                     /gene="hemB"
                     /locus_tag="ACHKIR_004250"
     CDS             10378..11352
                     /gene="hemB"
                     /locus_tag="ACHKIR_004250"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HFO9991907.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(11392..12243)
                     /gene="tauD"
                     /locus_tag="ACHKIR_004251"
     CDS             complement(11392..12243)
                     /gene="tauD"
                     /locus_tag="ACHKIR_004251"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HFO9991908.1"
                     /translation="MSERLSITPLGPYIGAQITGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIQTPPAGAILAAKELPSTGGDTLWASGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEAEHQRWREAVAKNPPLLHPVVRTHPVTGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWCWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(12240..13067)
                     /gene="tauC"
                     /locus_tag="ACHKIR_004252"
     CDS             complement(12240..13067)
                     /gene="tauC"
                     /locus_tag="ACHKIR_004252"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HFO9991909.1"
                     /translation="MSVLINEKLHSHRLKWRWPLSRQVTFSIGTLAVLLTVWWAVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRILLALLAAVLIGIPVG
                     IAMGLSPTVRGILDPIIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            complement(13064..13831)
                     /gene="tauB"
                     /locus_tag="ACHKIR_004253"
     CDS             complement(13064..13831)
                     /gene="tauB"
                     /locus_tag="ACHKIR_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HFO9991910.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKCIEGPGAERGVVFQNEGLLPWRNVVDNVVFGLQLAGVEK
                     MQRLEIAHQMVKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSPGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(13844..14806)
                     /gene="tauA"
                     /locus_tag="ACHKIR_004254"
     CDS             complement(13844..14806)
                     /gene="tauA"
                     /locus_tag="ACHKIR_004254"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO9991911.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGASVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
BASE COUNT         3821 a         3931 c         3639 g         3721 t
ORIGIN      
        1 agaaaaaaac tcactgcatg gccatacaag ccaggaataa attagcttcc cgttcatcat
       61 ttttgtgatc ttgcgcacat cattgagata acgcactttc ttcaccagta cgttgcacgc
      121 tttgtcagca gaagattgca acattctgtc agaaggccgc gaatcccccg ttttgtcgtg
      181 gtaatgtatg cactttgtgt tagacgggat aggtttttaa gatggaaaaa ctgcgggtag
      241 gaatcgtttt tggtggtaaa tcagcggaac atgaagtgtc tctgcaatcg gcaaaaaaca
      301 ttgtcgatgc cattgataaa agtcgcttcg acgttgtgct gctgggcatt gataaacaag
      361 ggcaatggca cgtcagcgat gccagcaatt atctgctaaa tgcagacgat cctgcccata
      421 ttgcgttgcg cccttcggcg accagccttg cgcaagtgcc aggtaaacat gagcatcagc
      481 ttatcgacgc gcaaaacggt cagccgttgc cgacggtgga tgtcattttc ccgattgtcc
      541 acggtacgct gggcgaagat ggttccttgc agggaatgct gcgggtcgcc aatttaccgt
      601 ttgtaggttc tgatgttctg gcttcagcag cctgtatgga caaagatgtc accaaacgtc
      661 tgctgcgcga tgccgggctg aacattgcgc catttattac cctgacgcgc gctaatcgtc
      721 acaacatcag ttttgccgaa gtggagtcta aactggggtt accgctgttt gtaaaaccgg
      781 ctaatcaggg ctcttctgtt ggtgtcagca aagtaaccag cgaagaacag tacgcaattg
      841 ccgtcgatct ggcgttcgag ttcgatcata aagtgatcgt tgagcaaggg ataaaaggtc
      901 gtgagatcga atgcgcagtt ctgggcaacg acaatccgca agccagcacc tgtggcgaga
      961 tcgtactcac cagcgatttc tatgcctacg acaccaagta cattgacgaa gatggcgcga
     1021 aagtggtagt tccggcagcc attgcgccag aaatcaacga taagatccgg gcgattgccg
     1081 ttcaggctta tcagacgttg ggatgcgcag gcatggcgcg tgtagacgtg tttttaaccc
     1141 cagagaacga agtggtgatc aacgagatca acacactgcc tggcttcacc aacatcagta
     1201 tgtatccgaa gctgtggcaa gccagcggtc tgggttacac cgacctgatc acgcgtttga
     1261 ttgagctggc gctggagcgt cacgctgcgg ataacgcatt gaaaaccaca atgtaatttt
     1321 taaatgcctg ataactttta ttgggcattt aagactgatg gcaaacgtaa gattgcctga
     1381 tgcgctttgc ttatcaggcc taccagataa ttgcaatata ctgattttgc atgattttgt
     1441 aggcagggta aggcgttcgc gccgcatccg gcaaaaacaa cgagcacttt gtcagccccc
     1501 agaacgccca atcctctgtt attgggcgtt ttcagtttct tcatcgcgac gacgaataat
     1561 cagccccgcc agccagaaac taatcaccca cgtcaccaga ccgacggcat aggtctgcca
     1621 accttttgct tcaaatccca gtaaacccac cacgccatta agaatgaata taaggccaat
     1681 ggcgaacgca tagtagtgcc agtcacggcg gatttttaca ggcaagttca tggcagctcc
     1741 gttaacgaaa aacaacatcc ttgcatattc tgcaacatcc gtctgtggcg tagacaacat
     1801 ttccgtaatt gttaataaat ttaacttaag gttgtcattt tgtgactcac gacgaagatt
     1861 cacgcttcgg gaatattccc aacatcaatt taccatgatt ctgatattga caatcttaat
     1921 gatctgtcag acttgccata agccgctggt atcacaccag aagagaagca gatgacttcc
     1981 ggaggttttt atggctgatt tcaccctgtc aaaatcgctg tttagcggaa aatatcgcaa
     2041 tgcctcttca acgcctggca acattgccta tgcgttgttt gtgctgtttt gcttttgggc
     2101 tggggcgcaa ttgctgaacc tgttagtgca tgcgcccggc gtctatgagc gcttaatgca
     2161 ggtccaggaa acaggtcgcc cacgggtgga aattggttta ggtgtcggca ccattttcgg
     2221 gctgatcccg tttttagttg gctgcctcat ttttgcagta gtggcgctat ggctgcactg
     2281 gcgacatcgc cgccagtaag atttacgttg ccagatgcgg cctaaaacgc cttatctggc
     2341 atgacattcc tgtttgatca ctgtttcata caacgcgccc gccgttcgtc tacgcgcttc
     2401 gcaaaccagg ccgtagtcag gttgcgggtg attttcgggc tttccagttg aatgccaggc
     2461 aacatttcgc ggggtagtga tttacccgtt ttcgcttcgg caagttgata aactttcttg
     2521 tacagcgccg tctcctcaaa agagaagctg tcccctttct ctaactggcg gcgaatttca
     2581 ctgtcgctca tccccagttt tcccgccagt ttgcgcgttg ccagttcggt tttcccgggc
     2641 tctttactgt cgtaacgaat taaatcaccg tccagtgcca gcttcacgcc gctggcctta
     2701 ctgaccgcgt tctgaaacgc ggcattacgg ctggcgtacc agccagcgtt aaaatcagca
     2761 aaacggtata tcggcgcgct atagctggcg ggataattca gtaaatggta agtaccaaac
     2821 cacaacccgc cgcgacgact gaagacttcc tgacggactg taccgtccat tttccacgga
     2881 taccctttgg tatgctgttc ggcaaaggca atgctgacct gcatcggccc accggtgcgc
     2941 accggattaa gcgaaccaaa caacgtctgc cccattggca ccatgctgat cagatcgtca
     3001 aaaatcgcgc tcaattgctt ttcagtacgc accgaatcta accgttcgct atagcttttg
     3061 ccgtttggcg atttgatttt tagtgcggta tgcaccagaa aagcaggaat gtgcatccgt
     3121 tcagcacgac ggtcaatctc ttgccaggcg atcttgctta agcccggaac agccggatct
     3181 gcctgataat ttgactcctg ctgcgccacc gccagcaccg agcagacgtt ttccagcgtc
     3241 ggtgccagcc cctgactttc aaacgtggtg gcgagatctt tcgcccaggc atcgcggtct
     3301 ttcacgctcg caggcatttt ctgccgcacg acgctcgcca catctatcgc tttttcgccc
     3361 tttttcaacg gttgcggagc ctgactacta catcccgcta aaaccagaac cgccaacaat
     3421 gacagcgaag atagatgatt gacgcgcgac attacttctc ctttttagct caaggtatgg
     3481 gttacttcct gaattttgtc accatccagc tcatgttcaa agctgcgcag acgtttgtag
     3541 atagacatca actcaaccag tgtggtccat gagttaatca ggtactggaa agcaccgcga
     3601 acctgaccaa aaacgttagt aatctgcgtc atcaggccga gtgtaatcgt accggcaaca
     3661 attgacggaa acagcaagaa caaaccgaaa acgttatcga cctgcaaata gagaatgcgg
     3721 gcgatgttga aatacatata gtgaaaatag aggcggaaat agtttttccg tacggcgcta
     3781 aacagctcgc gtaccgtagg cggcgtcgca cgcgtggcat cgtcttcacc ataaaccagc
     3841 tctttacggt aggcagcctc tactcgctgg tttttaaact ccagccccgg cagtttgatc
     3901 cctaccactg ccagtaatcc ggtccccatc agcgaccaga cgatagcggc aatcaccaga
     3961 ccatacggaa tgtgcccgac aatcggcagc tccggcacat gtgcggagag cgttaccagc
     4021 accggcagga aggcgatcaa cgtcatgata gcgttgataa agctgacgcc catattctcc
     4081 agcgttgaag caaaacgcat ggtgtcttcc tgcacacgct gcgcggcccc ttcgatatga
     4141 cgcagttgtt gccagttcgc catgtaatat tcgttcatcg ccgtacgcca gcggaacacg
     4201 tagtgactga caaagaagtt gttcagcaca ctgatcacca ccgcgatcag cgcaatcccc
     4261 agaaagactc ccacttcgcg gtaaaattgt tcgatggtga ctttatgcgg cgaacttagc
     4321 gccgtttgaa tcagatcata gaacggcgca taccaggcgt tgacggcaac cccgacttcc
     4381 accaaaaacc aggtgacgaa gataatcagc gcagtaccga gtattgacca gtattgccaa
     4441 cggtgcgggc tatagataaa ccagaacaat gcaaaaagtc ctacgcaaac aatgtagtaa
     4501 gcgtaaaaaa tcaggaaatc caacgaccag aaacgcgcgg cgctaatcgg gatctgcccg
     4561 gaagcgccgg tgatacgcgc cacccaatcc cccccaccgg cttaccagaa gataacggca
     4621 atcaatgccc aaacaaaggc cgagagaaaa aacgttcccg gctttgggaa aaaagactta
     4681 aacatatgca ctcctgctaa cttcttatcg ttttaacaac tgctgtgtat taaccgcatg
     4741 accgctacaa agcgtcagcc aaatgaaacc gcccattgtg acccatagca aggcgcttag
     4801 ttcgccagcc tgagcataag aattgtttcg acggatttca caaaccaaag attgtttaca
     4861 taagcggatt aacacgcgca ggatcacaaa tgcaggcatt ggtgagcagg aaaaagatcg
     4921 tgtttacgcg ccgttacgtt tttaacacta ttattgcact aattcgccct ttgcaatcca
     4981 tcaatgagta gtataaatac gctcagttac cttcattcaa tctatggaca ccaccgttga
     5041 aacgtagtct gcttttttct gccgtgctgt gtgcggcgtc attgacctcc gtccatgcgg
     5101 cgcagccgat cactgaaccg gagttcgcct ctgatattgt cgatcgttat gccgatcata
     5161 ttttttacgg cagcggtgcc acggggatgg cgctggttgt tatcgacggt aaccagcgcg
     5221 tctttcgcag ttatggcgaa acgcgacctg gtaataacgt tcgcccgcag ctggattcgg
     5281 tcatacgtat tgcttccctc accaagctaa tgaccagtga gatgctggtg aaattgctcg
     5341 actaggggac cgtgaagtta aacgatccgc taagtaaata tgctccgcca ggcgcacgcg
     5401 taccgactta caatgggacg cccattacgc tggtgaatct ggcaacccat accagcgccc
     5461 tgccccgtga acagcccggt ggcgcggcac atcgtccggt atttgtctgg ccaacgcgcg
     5521 agcaacgctg gaaatacctt tctacggcga agctgaaagc cgcgccaggc agtcaggcgg
     5581 cgtactctaa cctcgcattc gacctgctgg cagatgcact ggcgaatgcg tccggcaagc
     5641 cttataccca gttgtttgaa gagcagatta cccgtccatt ggggatgaaa gacaccacct
     5701 acaccccctc accggatcag tgccgccgtt taatggttgc tgagcgcggt gccagtccgt
     5761 gcaataacac gctggcggca attggcagcg gcggcgtcta ttccacgcca ggcgatatga
     5821 tgcgctggat gcagcagtat ttgtcatctg atttttatca gcgcagtaac caggctgatc
     5881 gcatgcagac gttgatttat cagcgcgtgc agtttacaaa agtgattggc atggatgtcc
     5941 ccggcaaagc ggatgcgctt ggtttaggct gggtatatat ggccccgaaa gagggtcgtc
     6001 cagggattat tcagaagaca ggtggtggtg gcggtttcat tacctatatg gcgatgatcc
     6061 cgcagaagaa cattggcgca tttgtggtgg tcactcgttc gccattgacg cgcttcaaaa
     6121 atatgagcga tggcatcaat gacctggtga ccgagctaag cgggaataaa ccgttggtta
     6181 tccccgcatc ctgattaata ctctgaaggc aaacggttga tagcgaccag tttgccttta
     6241 ttcatttcga tatagccgcc tttacgcaaa gcagcgagaa cttccgcaac gacagaacgc
     6301 gatatacgtg tacgttgatg gatataattc atcacaccaa tacgtgatcg caattcttca
     6361 ttccagtcaa tcattgataa taacgtcgcg cggatttgtt cgtaggaatt atgcccaatg
     6421 agctgaacgt cgcgtaattc cagaattcta ttttgccagg ctaaccagta aaaagcgtct
     6481 cgccagagtt gattttgttc aataagagta atggtttgtt tggctggtaa atgatatccc
     6541 gcacagcttc cttctgatat taatttgtat ggtatatcgt ttttcattaa accatcagcc
     6601 agccccataa tataaggagc ctgggtaata ccgataagta cgttttcttc tctacgcaga
     6661 gagataacgc cctctaaaat aacgaaagta tcttcgctat tgacatcact ggaaaatata
     6721 acttgctttt cattattaaa ctcgaagcgc gtaccgtatc tggacaaaca tttatcgagc
     6781 ttaccaaatt cctgaagagg tttaactaca gataacattt gtgcgtcctt tgcagtaatg
     6841 cccgtcaaat ccttgacggg cattatttag attaaattac ctatttcttc ggagtgaaga
     6901 atattaccag gtatatttaa cacccacatt cgcggaccag tcttgatcta cgtcaccacc
     6961 accgaggtag ttagcatcgg tataggcgct gaagttcttg gtgaagctga actgagtacc
     7021 cagaccaaca cgtaccgcag acccttcagt accgttatcg atggaatcac cgttcacatc
     7081 gttatcgttg ttagagtcgt cgtagacgta agccagtttg aagtacggag tcagagcctg
     7141 gtcttcgctg taggtgaagg tataacctgc atctacaccc agttcataac gcatgctgtc
     7201 gtaagactga ccatcaactt tcatgtcgtt gctcagctgg taatcatcac cagactggaa
     7261 cagaccagaa acgctgccgt aaggagtcac gtaacctgca tcacccagtt tgaagtcgta
     7321 accggctttc aaaccaaagc cccaagcgtc ggagttggtg ctgccgtcaa cgtaagtacc
     7381 gttgctcatg gttgcagaca ggtcgttgtt gaagtgagag tagctcaagc taccgtcaac
     7441 aaagacgttg ttcgcgaagt gagcagaaga gtagatgtag gcagtctggc tgtcttgatc
     7501 cacctgacca gaacggtcat tcatatcacc tttagcgaag cctgcagccg caccgacgat
     7561 ccacttagcg ttgttaccgt caattttggt atcaacaccg accatgatgc cgttaacatc
     7621 ctgatcatag ttgatggtgc cgttgtcgcc gttgaagtta ccaccgaagt agcttaccca
     7681 tgcgccgccg ttatcagcca gaccatgacg agagttggtc agacgagtac caacggtgtc
     7741 ttgttccagg ttccagatat tggtgttcgc agacgggatg ctcagcgcca tgttagcgta
     7801 gtcggtcagc tccatctgtt gcagaacaac ggtgttacca cgctgctcag cctgataggt
     7861 gtatgcaccc aggtcagctt tgttagcagc agagaaggtc gcgttgctgt ttttgtcgtt
     7921 gacgtagatc agctcgttgc cgttgtagtc agcgatagaa ccagcaccag tagcgttgtc
     7981 gatacgaact ttgtagttac cggttgcagc tgcaacatgg tcaccgttgt tatcaacttc
     8041 gttctgacca gcttcgatag aagccgtgtt gtcgttatcg ccgttaccgt taatggtcag
     8101 gtgaccatca gagttcattg cgattaagcc ataaccgtag tttgacttgg tagtatcgtt
     8161 ggtacggtcg ttggtcagat cggcatcgag tacgaagcgg ttgctttcga tgttgaacac
     8221 acctgcgtga atgttaccac tggtatcagt agtgctggtc aggttcagag tatcaacctg
     8281 cagagcattc gccgcagtgt tacctacatc caggataccg tcgttagtca ctttgatgtt
     8341 gttagcatac agagcagcat cacctacagc ccagccagca gaaacttcat cagaaatggt
     8401 tacggtactg ttgtcgacag tcagtgaatc agttgcaaca tgaccatctt cgttgatgtt
     8461 cagcgcagaa agaccagtca gagagataga gtcagaaatc agcgttgaat cagcaacatt
     8521 aacagttgaa ccactgttga tgctcagagt atcaatcaga gaagttttgg tggtatccca
     8581 ctgtgaaccg gtgttcaggg tcacattgaa cagaccgctc tggtaaactt cgttaccgat
     8641 gatgtgatca aattcgctgt attcagagtc gctgttttct acaccaacag ttgatgaagg
     8701 ccacaggctg ttagctgcga tgtcgatcag agttgcagtg gcttcagtat tgattccata
     8761 tgtataggag tcatttacac catcgtcatc agtatcaaca ccaatctgat gcgcagacat
     8821 tgccgcaccg acccacttgc tgccattgtt cagggtcaga tccaggcgat cagtaccatc
     8881 ccaaccgtta gtatctacat caccgtcagc atcacgatag ctatcacgac cttgtgggaa
     8941 gaagttctca tcaaaattac ttgagaagaa tacatcgccc atcagtgtgg aattgtcgaa
     9001 tgttgcagtg gtttgcatgg cgttatcagc atatggatgt gcaataacag ctaatgcaac
     9061 atcatcagca ttccacccat caccattgta atcactagca ttaccagtat taccaaacca
     9121 accagtagta ccttcatcag cccatgaacc agaggtcacg gtagaatctt taacagtgat
     9181 ggtgttatta aatacttcgc tgctgtttac accagtgctg acagtgttgt cgtcgatatc
     9241 ttcccactca taaccctgag tcagcgtaat acctgctaca tgagagttat tagagatgga
     9301 cagatcgact tcctgatcca gagtgatggc agtaccaatg ttaaatacat ttacaacatg
     9361 agtgtcagcg gcattattgt aagtgccgtt atatgtgtag tgttcgtagt tgtcatcgat
     9421 cgttgaatta tcaacggtca gcgccagacg atcatggtag tagtcatccg cacgatcagc
     9481 acaatcagtt gtcatgcact cggaataaat cataccgtga atagtgctgt ttttgattgt
     9541 caggctattg gcgttggtat tagtagaaat accatcgtcc agatagtagg tagaaatcac
     9601 gccgttaacg gtagcgttat taatgaccgg atagatatcg ccgttgtaat aactatcagc
     9661 ggcatagtta ttccagccta cataaccatc atagtaaacg tcatcagcat aggtggcgtc
     9721 gttgtagtga tggaaagtat tgtaagtttg accagaaata tcgttggtcg ctgcattagc
     9781 ctgagaggtg atagccagag tgcaagccaa tgctaattgt gatactacaa gtttcttttt
     9841 ccaggagtgc atttgtcatc cctcctcagg gacgtaataa gttgatccat caattatcaa
     9901 tgcatagaaa acatattatg tttcgcgttt acgattatgc ggcgttatag aggaatggtt
     9961 caattattct ttcccctaag tccgattgcg gacatatata aagcacgtga acacacacaa
    10021 agaaagaaat aacaaaaaca caatatttca atacgttaac gaaagcgtta attttaaaac
    10081 atagaccatg aaaatataaa acaaccaaaa cagacaaata atgtatcaat aagcaatgaa
    10141 ctttaagtta ttcacttttt gaaaacgtca ccaaatcccc aaaaacaata atctatggct
    10201 ataaataaaa attaaaaata ttttcagtga atatgaaatt attgctattt ctattacaaa
    10261 gatgtcctga cctctttttc attctcttct tgtcaacatc gcgacaactt tcgtaaaaca
    10321 cacccagcct gcttcaggta tactatgctc ctcgattcca caaacatcag gcagaccatg
    10381 acagatttaa tccaacgccc tcgtcgcctg cgcaaatctc ctgcgctgcg cgctatgttt
    10441 gaagagacaa cacttagcct taacgacctg gtgttgccga tctttgttga agaagaaatt
    10501 gacgactaca aagccgttga agccatgcca ggcgtgatgc gcattccaga gaaacatctg
    10561 gcacgcgaaa ttgaacgcat cgccaacgcc ggtattcgtt ccgtgatgac tttcggcatc
    10621 tctcaccata ccgatgaaac cggcagcgac gcctggcggg aagatggact ggtggcgcgt
    10681 atgtcgcgca tctgcaagca gactgtgcca gaaatgatcg tcatgtcaga cacttgtttc
    10741 tgtgaataca cttctcacgg tcactgcggt gtgctgtgcg agcatggcgt cgacaacgac
    10801 gcgactctgg aaaatttagg caagcaagcc gtggttgcag ctgctgcagg cgcagatttc
    10861 atcgcccctt ccgctgcgat ggatggtcag gtacaggcga ttcgccaggc gctggacgct
    10921 gcgggcttta aagatacggc gattatgtcg tattcgacca agttcgcctc ctccttttat
    10981 ggcccgttcc gtgaagctgc cggaagcgca ttaaaaggcg accgcaaaag ctatcagatg
    11041 aacccaatga accgtcgtga ggcgattcgt gagtcactgc tggatgaagc ccagggcgca
    11101 gactgcctga tggttaaacc tgccggagca tacctcgaca tcgtgcgtga gctgcgtgaa
    11161 cgtactgaat tgccgattgg cgcgtatcag gtgagcggtg agtacgcgat gattaagttc
    11221 gccgcgctgg cgggtgctat agatgaagag aaagtcgtgc tcgaaagctt aggttcgatt
    11281 aagcgtgcgg gtgcggatct gattttcagc tactttgcgc tggatttggc tgagaagaag
    11341 attctgcgtt aatgatcatt atgccgggtt atccaacccg gcatttcccc cttaccccgc
    11401 ccgataaaac ggtttatccc caagaatcgt cgcccgatgc attatccgcc gctgtggcag
    11461 gtaatccgca ttggcatagt gctgggttac gcggttatcc caaatcgcaa tatcatttgg
    11521 ttgccagcac cagcgcacct gaaactccgg tttggtgatg tgggcaaata aaaagcccag
    11581 caaggcttcg ctctctttct cgctgacatc aacaattcgc gtggtaaagc cttcattcac
    11641 aaacagcgcc tgtttaccgg tcaccggatg cgttcgcacc accggatgta gcaacggtgg
    11701 atttttcgcg accgcctcac gccagcgttg atgctcagcc tcggttttgc ggtacttgta
    11761 ttccgggaac gatttacgga aatcatgctc cgcacgcaac ccgctcaaca gctggcggaa
    11821 gggaacagag agcgcctcat atgcggcaat accactggcc cagagcgtat cgccgccggt
    11881 cgagggtaac tctttcgccg ccagaatcgc ccctgcgggt ggcgtttgaa taaatgtcac
    11941 atcggtgtgc cagttgtcgt tatccggcgg attatcgtta tgggtatcca ggacgatgat
    12001 ctcgtcaacc ccttcagcat gagggtaaac agggtgaata tgcagctcgc caaaacgctg
    12061 ggccagcgcg cgttgctgct gcggcgtaat agcttgatcg cgcagaaaca ccacctgatg
    12121 gcgcagcacc gcatggtaaa gctgttcaaa ctgattatcg cttaacgggc gcgttaagtc
    12181 ggcccccgta atttgtgcgc caatatacgg ccccagcggg gtaatgctca gacgttcact
    12241 cattgtactt ctccatgcca gggcgtcagg cggcgctgta acgcgcgcag acccagttct
    12301 aaaagaaagg cgataatcgc aatcaccgcg atccccgcca gcaccacgtc agtcgcgaga
    12361 aattcacccg ctgactgaac cataaatcct aaaccgcgcg ttgcggcaat cagctccgcc
    12421 gccaccagcg tagaccagcc cacacccaga ccaatgcgta atccggtgag gatttccggc
    12481 agcgcgccgg gcaaaatgac aaaccacagc acctgcgcac ggctggcacc cagcgactgg
    12541 gcggcacgaa tgcgaacctg ctgcgcgctt ttcacccccg ccagcgccga catcgccacc
    12601 ggcgcaaaaa tcgctaaata gatcagtaag atcttcgagg tttcgccaat accaaaccag
    12661 atcaccatca gcggcaaata agccagcggc ggcaccggac gataaagctc gattatcgga
    12721 tccagaatgc cgcgcaccgt cgggcttagc cccatggcga tcccgaccgg aataccgatc
    12781 aacaccgccg cgagtagcgc cagcaaaatg cgcgtcaggc tggctgccag atgctgccac
    12841 agcgtggcgt ccataaatcc ctgcggcccg gcaatggtga gcagttttgc cagtacctgt
    12901 tgcggtggcg gtaaaaacag cgggctaatc agttgcagcg ccgcgactgc ccaccataca
    12961 gtgagcaaaa ccgctaacgt accaatgctg aaggtcacct gacgcgagag cggccagcgc
    13021 catttcagcc gatgcgaatg cagtttttca ttaatgagca cactcatgag aacgcctccc
    13081 gttgctcaaa tacgcggctt aaaacatatt cgcgcatggc gatgaattgt ggatcggact
    13141 tgatgctgcg gctcgactct cccgcaacaa agcggcgagc aaagttgagc ggcagccgct
    13201 ccagcacacg gcctgggccg ggtgaaagca gaaccagttc agtcgccata aacaccgctt
    13261 cttctatatc gtgggtaatc aacagcacct gctttcccgt ctcctgccag agtttcagca
    13321 gcagggtttg catctggtcg cgggtgaagg cgtccagcgc accaaacggt tcgtcgagta
    13381 ataacagctg gggatttgcc gccaacgcgc gggcaatccc cacccgctga cgttgaccac
    13441 cggaaagctg ccagatgtag cgtttttctg cgccttccag ccccactttt ttcaccatct
    13501 ggtgtgcgat ttccagtcgc tgcattttct ctacacctgc cagttgcagg ccgaacacca
    13561 cgttgtctac cacattacgc cacggcaaca gcccttcatt ctgaaaaacc acgccccgct
    13621 ctgctcccgg tccctcaatg cacttacccg ccagttgaat gctgccatgc tgataaggca
    13681 caaaaccggc aatcagattc agcaaggtgg ttttgccgca gccagacggc cccagaacta
    13741 ccagaagctc gccgctttcc agcgtcaggt tgatatcttc cagtgccggt ttgccgccat
    13801 aatcggcgta aagatgagaa atttgcagca tctgcgcctc cttttattgc acgaagcgcg
    13861 aggtaacgta ctggctgtaa tcattcgcca cggcggggac tttgccctgc tctttcaaaa
    13921 actgcgcggt gtcgatgatc gctttgttca ccggtccggt cagttctgcc gtttgctgct
    13981 gcggcgtcag ataggtattc cccttcacca gccccggaac gtcaccttca ggcacgccgc
    14041 ttaaccgcgc cagtttgctg atgttttccg gctgtttcag ccacgcgtct gggttagcaa
    14101 tgtacggttg ctgagcatcg atggcgcttt tagcgaacgc tttcacgacc tcaggatgtt
    14161 tctcggcaaa atctttccgc acgacccaga cgtccagcgt cggcgcgccc cactgcccga
    14221 cctgttcaga atcggtcagc accttgccgt ctttttccag cgcgttaacc gccggtgccc
    14281 agacataagc accatcaata tctccccgct gccaggcggc gataattgcg ggcggctgca
    14341 ggttcacaat ctccacttgc ccaggtttaa taccccagtg tttcagcgcc gccagcaggc
    14401 tgtagtgggt ggtggagata aacggtacgg cgatgcgctt gccaatcaga tcttccggtt
    14461 tgctgatagt tttcttcacc accagcgctt cggagttacc cagttttgac gccagtaaga
    14521 agacttcaat cggcacctgt tggctggctg ccaccgctaa tgggctggaa ccgaggttac
    14581 cgatttgcac gtcgccagaa gccagcgccc gcacgatgct ggctccgctg tcaaacttac
    14641 gccagtcaac gctcgccccg ctctctttag caaaggtgtt gtcggcctga gccactttcg
    14701 ccggttcggc tgaagtttga tacgccacag tgacgttcac cgcctgtgcc tgaaaagcga
    14761 tgaatgccag tgcggcaaga agtgtgttac gcgatgaaat tgccatgatt gtctgctccc
    14821 ctgtcttgtt atgggagcag tattcaggaa taaaaacatt cattaaaaga attagttgtt
    14881 atcgcacaga tgattttatt cttagcgaaa aagtggcaaa ggaaagatta aatatgcaat
    14941 tacacccact ctgacaattc agtaagttac tgcacttcat tttattaatg cataaacaag
    15001 cagattataa tgtcttttat tttattaaca gcaatacata taatgaaacc attatattca
    15061 cattgcatat ataactgtat cattctcaat caaccacata atacattcac at
//
LOCUS       DAZGQL010000115        15091 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.115, whole genome shotgun sequence.
ACCESSION   DAZGQL010000115 DAZGQL010000000
VERSION     DAZGQL010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15091
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.115"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..180
                     /locus_tag="ACHKIR_004255"
     CDS             <1..180
                     /locus_tag="ACHKIR_004255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9991912.1"
                     /translation="CRDNDHKNDNMKINPGMKGKKNGLPGEAVTKYAWFYAGCGVNAF
                     SGLRGSAVRMKRIRR"
     gene            complement(170..1222)
                     /gene="fbaB"
                     /locus_tag="ACHKIR_004256"
     CDS             complement(170..1222)
                     /gene="fbaB"
                     /locus_tag="ACHKIR_004256"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HFO9991913.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            1479..2756
                     /locus_tag="ACHKIR_004257"
     CDS             1479..2756
                     /locus_tag="ACHKIR_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HFO9991914.1"
                     /translation="MKTTAKLSFMMFVEWFIWGVWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGAALMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQMLGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            2753..3757
                     /locus_tag="ACHKIR_004258"
     CDS             2753..3757
                     /locus_tag="ACHKIR_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFO9991915.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAYFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPDLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRITTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGVNAIDPALKAELDAVNQLDFNRYATALAKYRQQREAI
                     "
     gene            3754..4719
                     /locus_tag="ACHKIR_004259"
     CDS             3754..4719
                     /locus_tag="ACHKIR_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HFO9991916.1"
                     /translation="MSGARLHTLLPELATRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEELQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAATLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(4693..5439)
                     /locus_tag="ACHKIR_004260"
     CDS             complement(4693..5439)
                     /locus_tag="ACHKIR_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO9991917.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(5491..6309)
                     /locus_tag="ACHKIR_004261"
     CDS             complement(5491..6309)
                     /locus_tag="ACHKIR_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HFO9991918.1"
                     /translation="MQLRITSRKKFTALLCALGLISIVAIYPRQTVNFFYSTAIQIKD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVSASVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKRTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTVEELQGFVDGIKETP"
     gene            complement(6374..7174)
                     /gene="thiD"
                     /locus_tag="ACHKIR_004262"
     CDS             complement(6374..7174)
                     /gene="thiD"
                     /locus_tag="ACHKIR_004262"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFO9991919.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAQRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWTDTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            complement(7171..7959)
                     /gene="thiM"
                     /locus_tag="ACHKIR_004263"
     CDS             complement(7171..7959)
                     /gene="thiM"
                     /locus_tag="ACHKIR_004263"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HFO9991920.1"
                     /translation="MQVDLLSSAQSAHTLHLFHQHSPLVHCMTNDVVQTFTANTLLAI
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMSAAVEQATRSQTPWTLDPVA
                     VGALDYRRRFCVELLSHKPTAIRGNASEIMALAGVANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENIASACHWMKQAGERAVASSEGPGSFVPHFLDALWQLTQEVQA"
     regulatory      complement(7994..8090)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(8182..8454)
                     /gene="rcnR"
                     /locus_tag="ACHKIR_004264"
     CDS             complement(8182..8454)
                     /gene="rcnR"
                     /locus_tag="ACHKIR_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HFO9991921.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     gene            8576..9400
                     /gene="rcnA"
                     /locus_tag="ACHKIR_004265"
     CDS             8576..9400
                     /gene="rcnA"
                     /locus_tag="ACHKIR_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HFO9991922.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIISTA
                     FWMFWRTWRGKRNWLENMHEHDHEHHHHDHEDHHDHGHHHHHEHGEYQDAHARAHAND
                     IKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSIGLA
                     LTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            9619..9957
                     /gene="rcnB"
                     /locus_tag="ACHKIR_004266"
     CDS             9619..9957
                     /gene="rcnB"
                     /locus_tag="ACHKIR_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HFO9991923.1"
                     /translation="MTIKNKMLLGALLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPDMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(10039..11073)
                     /locus_tag="ACHKIR_004267"
     CDS             complement(10039..11073)
                     /locus_tag="ACHKIR_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HFO9991924.1"
                     /translation="MEIRIMLFIVMMMVMPVSYATCYSELSVQHNLVVQGDFALTQTQ
                     MATYEHNFNDSSCVSTNTITPMSPSDIIVGLYNDTIKLNLHFEWTNKNNITLSNNQTS
                     FTSGYSVTVTPAASNAKVNVSAGGGGSVMINGVATLSSASSSTRGSAAVQFLLCLLGG
                     KSWDACVNSYRNALAQNAGVYSFNLALSYNPITTTCKPDDLLINLESIPVSQLPATGN
                     KATINSKKGDIILRCKNLLGQQNQTSRKMQVYLSSSDLLAGSNTILKGAEDNGVGFML
                     ESNSSPVTFLNITDNSKGYTNLKEIAAKSKITDTTVSIPVTASYYVYDKNKVKSGALQ
                     ATALINVKYD"
     gene            complement(11089..13569)
                     /locus_tag="ACHKIR_004268"
     CDS             complement(11089..13569)
                     /locus_tag="ACHKIR_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFO9991925.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREIIKRLGINTDNFASGK
                     QCLTFEQLVQGGSYTWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNSKSTYVRFNSGLNLLGWQLHSDASFSKTNSNPGVWKSNTLYLERGF
                     AQLLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGSMVANNYYAFTLGTGW
                     NTRIGAISVDATKSHSIQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWANNKDNYRRDENDIYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNLRRISYTLAASHAYDENHHEEKRFNIFISIPFDWGDDV
                     TTPRRQIYMSNSTTFDDQGVASNNTGLSGTVGSRDQFNYGVNLSYQHQGNETTAGANL
                     TWNAPVATVNGSYSQSSTYRQAGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPFRGAVVL
                     VNFDTDQRKPWFIKALRTDGQPLTFGYEVNDIHGHNIGVVGQGSQLFIRTNEVPPSVN
                     VAIDKQQGLSCAITFGKEIDESRNYICQ"
     gene            complement(13585..14259)
                     /locus_tag="ACHKIR_004269"
     CDS             complement(13585..14259)
                     /locus_tag="ACHKIR_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO9991926.1"
                     /translation="MKGLLALLISSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSENWVTISDVKANNVKVNYETIMIAPLESQSINVKSNNANNWHLTIIDDHGN
                     YISDKI"
     gene            complement(14340..14882)
                     /locus_tag="ACHKIR_004270"
     CDS             complement(14340..14882)
                     /locus_tag="ACHKIR_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO9991927.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGYYLYLGDNSDVLDISAPFNIESYKTAEGQYAIPFKAKYLKLT
                     DNSVQSGDVLSSLVMRVAQD"
BASE COUNT         3751 a         3782 c         3490 g         4068 t
ORIGIN      
        1 tgtcgcgata acgatcacaa aaacgacaat atgaaaatca atccaggaat gaaaggcaaa
       61 aaaaacggcc tcccggggga agccgtaaca aagtacgcgt ggttttatgc cggatgtggc
      121 gttaacgcct tttccggcct acggggtagt gctgttcgta tgaagcgcat caggcgatag
      181 taattttgct atcgagataa acgtcctgca cggcgttaat cagtttcacg ccgtcagcca
      241 tcgatttctt gaacgcctta cgtccaagaa tcagccccat tccgcctgcg cgtttgttga
      301 taaccgcagt acgcactgca tcgctgaggt cagtttcacc gcctgcagca ccgccggagt
      361 ttatcaaccc ggcccggccc atatagcagt tagctaactg ataacgcacc aggtcaatcg
      421 ggttttcact ggtcagtttg ctgtagactc gatcgtcggt ataaccgtaa ttaatcgctt
      481 tatagccgcc gttattttcc gccatttttt gtttgacgat atctgcacct atggttgccg
      541 ccagatggtt cgcctgacca gtcaggtcgg cggaaacatg gtaatcaacg ccatctttct
      601 taaaggcgga gttacgcaaa taggcccaca gcactgtcac catgcccagc tcgtgcgcac
      661 gttcaaaagc cgcagaaatt tcttcaatct ggcgacgtga ctcttccgag ccaaaataga
      721 tagtcgcacc aaccgccacc gcgcccatat tgaacgcctg ctccacgctg gcatacagcg
      781 tttgatcgta ggtattcggg taacttagcg tctcgttgtg attaagtttg acgaggaatg
      841 gaatgcgatg cgcgtaacgc cgcgataccg atgccagcac gccgtaagtt gacgccacac
      901 agttacagcc cgcttcgatc gccagttcaa caatgttttt cgggtcaaag tagagcgggt
      961 tagcggcaaa tgaagctccg gcagagtgct caacgccctg gtcaaccggc agaatagaaa
     1021 gatatcctgt gccagccaga cgcccggtgt tgtacaacgt ctgcatatta cgtaacaccg
     1081 ctggcgggcg attattgtca atcatcacgc ggtctacgta gtcatgtccg gggagataaa
     1141 gctggtcaga aggaatagtc atacaacggt gctgtaaaag gttgtcggcg tctttgccaa
     1201 gcaactgcgc aatatctgtc attactatgc tcccgtaaat tccgattgga tatcggctat
     1261 ggattttcct ggcccgcctt ttgcgggcaa tcataatcct ggtcgttaca ggcgagtttt
     1321 tccatctttt ggatgttttt tcagcgtttt ctgttggcac gattcatcag aaaaaatgtt
     1381 atcgcggtca agttttcacc gcaaaggtat ttaaaaggta ttattaaatg gtattgtcat
     1441 cgcgtacctt acattacctg tcatgaagga attaaaagat gaaaacaaca gcaaagctgt
     1501 cgttcatgat gtttgttgaa tggtttatct ggggcgtgtg gtttgtgcca ttgtggttgt
     1561 ggttaagtaa aagcggtttt agtgccggag aaattggctg gtcgtatgcc tgtaccgcca
     1621 ttgcggcgat cctgtcgcca attctggttg gctccatcac tgatcgcttt ttctcggcgc
     1681 agaaagtgct ggcggtattg atgttcgctg gtgcggcgct gatgtatttc gctgcgcaac
     1741 agaccacttt tgccgggttc ttcccgttac tgctggccta ctcgctaacc tatatgccga
     1801 ccattgcgct gactaacagt atcgcttttg ccaacgtgcc ggatgtggag cgtgatttcc
     1861 cacgcattcg tgtgatgggc actatcggct ggattgcctc cggtctggca tgtggatttt
     1921 tgccgcaaat gctgggttat gccgatatct ctccgactaa catcccgctg ctgattaccg
     1981 ccggaagttc tgctctgctc ggtgtgtttg cgtttttcct gcccgacacg ccgccaaaaa
     2041 gcaccggcaa aatggacatt aaagtcatgc tcggcctgga tgcgctgatc ctgctgcgcg
     2101 ataaaaactt cctcgtcttt ttcttctgtt cattcctgtt tgcgatgcca ctggcgttct
     2161 attacatctt tgccaacggt tatctgacag aagtcggcat gaaaaacgcc accggctgga
     2221 tgacgctcgg ccagttctct gaaatcttct ttatgctggc attgccgttt ttcactaaac
     2281 gctttggtat caaaaaggta ttgttgcttg gtctggtcac cgctgcgatc cgctatggct
     2341 tctttattta cggtagtgcg gatgaatatt tcacctacgc gttactgttc ctcggcattt
     2401 tgctgcacgg cgtcagttac gatttttact acgttaccgc ttacatctat gtcgataaaa
     2461 aagcccccgt gcatatgcgt accgccgcgc aggggctgat cacgctctgc tgccagggct
     2521 tcggcagttt gctcggctat cgtcttggcg gtgtgatgat ggaaaagatg ttcgcttatc
     2581 aggaaccggt aaacggactg actttcaact ggtccgggat gtggactttc ggcgcggtga
     2641 tgattgccat tatcgccgtg ctgttcatga tttttttccg cgaatccgac aacgaaatta
     2701 cggctatcaa ggtcgatgat cgcgatattg cgttgacaca aggggaagtt aaatgaaaac
     2761 agaacgtatt ctcggtgctc tttatgggca ggcgttaggg gatgcgatgg ggatgccctc
     2821 cgagctttgg ccgcgcagtc gcgtcaaagc gtactttggc tggattgacc gttttctgcc
     2881 tgggccaaag gagaataacg cggcctgtta ttttaaccgc gccgaattca ccgacgatac
     2941 ctcgatggcg ctgtgtctgg cggatgcgtt actggaacgt gaaggcaaga tcgatccgga
     3001 tctgattggg cgtaatattc tcgactgggc gctgcgtttc gacgccttta acaaaaacgt
     3061 actaggtccg acctcgaaga ttgctctcaa cgccattcgc gacggtaaac ccgttgctga
     3121 actggaaaac aacggcgtga ccaacggcgc agcgatgcgc gtctcgccat taggttgttt
     3181 gcttccggcg cgtgatgttg attcctttat tgatgatgtg gcgctggcct ccagcccgac
     3241 acataaatcc gatctggcgg ttgcgggggc ggtagtcatc gcatgggcga tttctcgtgc
     3301 cattgacgga gaaagctggt cagcgattgt tgattcactg ccttcaattg cgcgacatgc
     3361 tcaacaaaaa cgcatcacta ccttcagcgc ctcactggca gcacgtctgg agattgcgct
     3421 gaaaattgtg cgcaatgccg acggcaccga atccgccagc gaacagcttt accaggtcgt
     3481 tggcgcaggt accagcacta ttgagtccgt tccgtgcgcc attgcgctgg ttgaactggc
     3541 acaaaccgac ccgaaccgtt gcgccgtcct gtgcgctaac cttggcggcg acacagacac
     3601 catcggtgct atggcgacgg caatttgtgg cgcgttgcat ggcgttaacg ctatcgatcc
     3661 tgcgttaaag gcggaactgg atgcagtaaa tcagcttgat ttcaaccgct atgccacggc
     3721 gctggcgaag tatcgccaac aacgggaggc gatatgagcg gcgctcgatt acacacgctg
     3781 ctgcctgaat tagccacgcg tcagccagtg atggtcgtcg gcgcggcggt cattgatgtg
     3841 atcgccgacg cttatgccct cccctggcgt gggtgcgata tcgaactgaa acagcagagc
     3901 gttaacgttg gcggctgcgc actgaatatt gccgtggcgt taaagcgcct cggcatcgaa
     3961 gcgggtaatg ccttgccgct cggtcagggc gtgtgggcgg agattattcg caaccggatg
     4021 gcaaaagagg gcttaatcag cctgatcgat aacgccgaag gtgataacgg ctggtgtctg
     4081 gcgctggttg agccggatgg cgaacgcact tttatgtcat tcagcggtgt ggaaaatcag
     4141 tggaatcgcc agtggctggc gcgattaacc gttgcgcctg gcagcctgct ctatttttcc
     4201 ggttatcaac tggcctcgcc ctgcggcgaa ttgttagtgg aatggctgga agagctgcaa
     4261 gacgtgacgc catttatcga ttttggccca cgtattggcg atatcccgga tgcattactg
     4321 gcgcggatca tggcctgtcg acctttagtg tcactcaatc gtcaggaggc tgagattgcc
     4381 gccgaacgtt ttgctttatc cgcagagata acaacacttg gcaagcaatg gcaggagaaa
     4441 tttgctgcga cgttgatcgt tcgtctcgat aaagagggcg catggtattt cagcaacgac
     4501 gcttctggct gcattccggc atttccaacg caagttgtag acaccattgg ggcgggcgac
     4561 agtcatgccg gcggcgtact tgccgggctg gcctctggtc tgccactggc ggatgccgta
     4621 ttactgggca atgcagtggc gtcgtgggtt gtcgggcatc ggggcggtga ttgtgcgcca
     4681 acgcgcgagg aactactcct cgcacacaaa aacgtataga tcgctgcgac agtggctgat
     4741 gctgtactca ataggccgtt gctgttggtc aagcgccact tgcttgatca ccagcaccgg
     4801 tattttgcta tctaactgaa tatgtgactg aaactcggca tccggcatcc gggcgctaac
     4861 gcgggaacgc gtacgttgtg ggtaaatgtg ctggctgcgg aagtagtcat aaagagaaat
     4921 cccgatggca tcaacatcat gaattaaatg tgccggaacc cacgattcct caatcgatac
     4981 cgcctcttca tcgacatagc gaatacgctt aagcaaaaag acatcacttc ctgcttcgac
     5041 cgctagctgc tgcgcgactt cttcgggaca tttcacgaca cgcttgttaa cccatagcgt
     5101 atcgggcttt ttcccgcgca ataccacctg ctgagaaaaa cctcgcgctt ctttcaacga
     5161 gtattcgaag atgttgttga tctgcgtgcc gtaaccacgc gaacgcgtca ccacgccctc
     5221 ttcttccagc gcctgcatcg ccttgcgcac cgtaatgcgc gaaacgccgg ttaactgact
     5281 taaatcacgc tcaccgggca aaatattgcc atgctccagc accccgctgc gcacggcatt
     5341 ttttaccgtt tcggcaaact taatataaag cggcgtgtta tccgccgcta aaatacgttc
     5401 gttcagttgg gcaatcagct gggtatgcgc ttgttccatt tgtctttttc ctgccaggga
     5461 gtctggtggg agtatatcac tgcttcttat ttaaggcgtt tctttaatcc catcaacgaa
     5521 tccctgcaac tcctccaccg tgccattaaa cacattaaaa tccaccggac cattaatgcc
     5581 atctacctgt ccacggtcgg aatgctgcca gaagcgccag gccattccgt cattgtccgg
     5641 acgacgttga tagtagtgag ccacccacca cggatattca ttaaaatagc ccgccagatt
     5701 ggtgtgataa aaaacggctc ctgagtaaat aattggcttt tttcccgtac gtttttcgac
     5761 catttttagc cactgactta cccgcttgcg taattctttt gccgataatt ttcctcgctc
     5821 ttctacgtcc agtaccgcag ggaaatcgcc ttgtgagaaa tccaccgttt gcagaaataa
     5881 tcgcgcctga actgaagcgg atactgacgg ggagaaataa tgatacgccc cgcgcagcag
     5941 gccattttcg cggcttagtt gccagttacg cgaaaaatag gggtccacca gcttttcgcc
     6001 ttccgtcgcc ttaataaaag caaactgtaa gcggataccg ttgtcgcgca tttttgccac
     6061 ccgctgccag tcgatccgct cctgccagcg cgaaacatct attccgtgaa tggtgtaact
     6121 ggcaggaatg cgaatagcga aagatttaac cgggcgataa ccgtagaagt ggatgtagtc
     6181 tttaatctga attgctgtcg aatagaaaaa attcaccgtc tgacgcggat aaattgcaac
     6241 gatggaaatt agcccaagag cgcataataa agcggtgaat ttttttcgac tggtgattct
     6301 tagctgcatt ggtttcatcc ctgaatgtca gcgccaaagg ctgacaataa ccaaagcagc
     6361 tatagtacgg tgctcaccac caggcgtgga agtggtgaac cggaccaata ccgtgaccaa
     6421 cttccagcgt gtcggcctgg gctaatgccg atgaaagcca gctttttgcc tcctgtacgg
     6481 tgtcagtcca gtttgtatgg cgcgggcgca gtgcagccaa cgccgcagag agcgtacaac
     6541 cagtgccgtg agtgtttttg gtcataatgc gctgtgcggt aaaccgttgt tcaccctcgc
     6601 gggtaaacag ccagtccggg ctttgctcat catccagatg accacctttc attagcactg
     6661 cgccacagcc catcgccaac agcgatcgcc cttgttccag catttcctgt tcggtgcgcg
     6721 cgtgtggcgc gtcgagcaag gcggctgctt cgggcaagtt tggcgttatt aatgaaacct
     6781 gtggcaataa tcgactgcgc agcgtagcaa ccgccgaagg tgaaagcagc gggtcgccgc
     6841 tttttgccag cataacggtg tcgagtacca cgttttggat ctgataacgt tgcaaccgtt
     6901 ctgccaccgc ttcaacaata tcggtttccg ccaacatacc gattttagtg gtgtcgattc
     6961 gcacatcgct gaacaccgaa tcgagctggg cggcgacaaa atcaggctca atgcgataca
     7021 ccgactgtac accacgggta ttttgcgcca ccagtgcagt aataactgag caaccataag
     7081 cgccaagtgc cgagaaggtt ttaagatcgg cctgaatccc cgcaccacca ctcggatcag
     7141 taccggcaat cgtcagagcg ttaattcgtt tcacgcttgc acctcctgcg tcaattgcca
     7201 gagcgcatca aggaaatgtg gaacaaaact gcctggcccc tcgctactgg cgactgcgcg
     7261 ttctccggct tgtttcatcc agtgacaggc agatgcgata ttttccagcg tatcgcctgg
     7321 tagcgcacaa caggcagcga caaccgccga taatgcacag ccagttccta ccactttggt
     7381 cattaacgga tcgccaccgt gaatgccaat gatacgatgt ccatcggtaa cataatccac
     7441 ctcgccagtg accacgacga ttgcgccagt ttcccgtgcc agtgtttgtg cagcgggtat
     7501 cgcgttagct gcggcatcag tggtatccac tccccgtcca ccattagcaa cgccagctaa
     7561 tgccatgatt tccgaagcat taccacgtat ggcggttggc ttatgggaca gaagttccac
     7621 acaaaaacgg cggcgataat cgagcgcacc aaccgcaacc ggatcaagcg tccagggcgt
     7681 ttgtgatcgt gttgcttgct caacggcagc actcatcgct tgcgctcgtg gctgcgtcag
     7741 cgtgccaacg ttaatcaaca aggcactggc gatagccgca aactgactgg cctcttcggt
     7801 ttcgataacc atcgctggcg atgcaccgat cgccagcaag gtattggcag taaaagtttg
     7861 caccacatcg ttggtcatgc agtgcacaag aggggaatgt tggtgaaaaa ggtgtaacgt
     7921 gtgcgcagat tgcgcggaac tcagcaggtc gacttgcata gtttgctcct gccataacgt
     7981 gaagaagcaa tgacctggtg gtccgtgact tccctacgct ggcattatcc agatcaggtg
     8041 atacgggtat ttctcagcct tcacaaagaa gggcaccccg agtcgtttgg ttgcgataat
     8101 aaatcgcaga ggaggatggt aatgtccagc gcacgcgttg taaacgggga aaaacggcgg
     8161 tattacaatc gcgttaataa attatttgat ataagaatcc agcaccttca gaacgacatc
     8221 cagatcttct tcacgtttta gctcatcccc ctggtgaacg atgtgttccg tcagatggcc
     8281 tttaatcact tcccgcatca gaccgtttac cgcgccacgg atagcagcaa tctgttgtaa
     8341 aactgcagcg cattcatgcg gttcgtcgag cattttcttg agcgccacga cctggccctg
     8401 aatcttactg gcacgcgctt tcagtttctg tttatcacgg attgtatgag acatggcaac
     8461 acctggttaa caagaatatg aaaaatcata gcactattaa tctactgggg ggtagtataa
     8521 ggtactgggg ggggagtaga atcagattgc cgaattaata ctaagaatta ttatcatgac
     8581 cgaatttaca acacttcttc agcaaggaaa cgcctggttc ttcatcccca gcgccatctt
     8641 acttggtgcg cttcatggcc tggaaccggg gcactcaaaa acgatgatgg cggcgtttat
     8701 catcgccatc aaaggcacca ttaaacaggc ggtgatgctc ggactggcag caactatttc
     8761 gcataccgca gtagtctggt taattgcctt tggcgggatg gtgatcagca aacgctttac
     8821 tgctcaatca gcagaaccgt ggctccagtt gatttccgca gtgatcatta ttagcaccgc
     8881 gttctggatg ttctggcgta cctggcgcgg caaacgcaac tggctggaga atatgcacga
     8941 gcatgatcat gagcatcatc accacgatca cgaagatcac cacgaccatg gacatcatca
     9001 ccatcacgaa catggcgagt atcaggatgc ccatgcacga gcccatgcca atgacattaa
     9061 acgacgcttt gatggtagag aggtcaccaa ctggcaaatt ttgttatttg gcttaaccgg
     9121 tggccttatc ccctgcccgg cagcaattac cgtgctgttg atttgcattc agttgaaagc
     9181 cctgacactg ggcgcaacac tggtcgtcag tttcagcatc ggcctggcgt taacgcttgt
     9241 caccgtaggc gttggcgcag caatcagcgt tcagcaggtc gcaaaacgct ggagcggatt
     9301 taacactctc gctaaacgcg ctccctattt ttccagtctg ttgattggct tagttggtgt
     9361 gtatatgggc gtacatggct tcatgggcat aatgcgataa ctcaatcctt taggcttgca
     9421 tgttatcttc agcccaggat gattcttaaa tcagctattt ccgctgactc tctgctcgct
     9481 aaggatgctt ttagggcatc ctttcattta cactttttac gaaatcatgg gatcactaac
     9541 aaaatatcgc ttgtcagtta tattgtatgg caggaaagat atgcgactga tattacagat
     9601 ccccaaagtg gagagtttat gaccattaaa aataagatgt tgctgggtgc gcttttgctg
     9661 gttaccagtg ccgcctgggc cgcaccagcc accgcgggtt cgaccaatac ctcgggaatt
     9721 tctaagtatg agttaagcag tttcattgct gactttaagc atttcaaacc aggcgatacc
     9781 gtcccagaca tgtaccgcac cgatgagtac aacattaagc agtggcagtt acgtaacctg
     9841 cccgcgcctg atgccgggac gcactggacc tatatgggtg gcgcgtacgt gttgatcagc
     9901 gacaccgacg gtaaaatcat taaagcctac gacggtgaga ttttttatca tcgctaaaaa
     9961 aagccccctc atcatgaggg ggaaatgcag acaccttgtt attttttatt attcgccact
    10021 tgctcgtctt gcttattatt agtcgtattt cacattgatt aatgcggttg cctgtagtgc
    10081 accagatttt actttatttt tatcataaac atagtaactg gcagttactg gaattgagac
    10141 cgttgtatct gtaatttttg acttcgccgc aatttctttt aaatttgtgt accctttgct
    10201 gttatcagtg atatttaaaa atgtgactgg cgaactattg ctttcaagca taaatccgac
    10261 tccattatct tctgcacctt tcaggatcgt gttactgccg gctaacaaat cagaacttga
    10321 taaatacacc tgcatttttc gtgatgtttg attttgttga cctaataaat ttttacaacg
    10381 cagaataata tccccttttt tactatttat tgttgctttg ttacctgtgg ctggtaattg
    10441 tgaaacggga atactctcta aattaattaa taaatcgtcc ggtttgcagg ttgtggttat
    10501 cgggttgtat gacaatgcca aattaaagga atagacacct gcattttgtg ccaatgcatt
    10561 tctgtagcta tttacacatg catcccatga cttgccacct aataaacaca gtagaaattg
    10621 tactgcggca ctcccgcgtg tcgatgatga agcactggat aatgttgcaa caccattaat
    10681 catcactgaa ccgccgcccc ccgcagaaac attcactttt gcattactgg ccgcaggtgt
    10741 caccgttacc gaataaccac tggtgaaact ggtctgatta tttgacaacg taatgttgtt
    10801 tttattggtc cattcaaaat gtaaatttaa tttaatggta tcgttataaa gtccaacaat
    10861 aatatccgac gggctcatag gtgtgatagt atttgtactc acgcatgacg aatcattaaa
    10921 attatgctca tatgtcgcca tttgtgtttg agtaagtgca aaatccccct gaacaaccaa
    10981 attgtgctga acagataact cactataaca tgtcgcatag ctcacaggca taaccatcat
    11041 cattactata aatagcatta tgcgaatttc catagaacca cctgactatt actggcaaat
    11101 ataatttcta ctttcatcaa tctctttacc gaaggtgatt gcgcatgaaa gcccttgttg
    11161 cttatcaatt gccacattaa ccgatggcgg tacttcattg gtgcgaataa ataactggct
    11221 tccctggccg acaacaccaa tattatgacc atggatatca ttgacttcat aaccaaacgt
    11281 taatggttgc ccatccgttc ttaacgcttt tataaaccag ggcttgcgct gatcggtatc
    11341 aaaattaacc agtacaaccg cgccgcgaaa aggggcggca attttccggt tgccacgtaa
    11401 ttctgcttcg ctatcgcttt gcgacacatc cagcatcagg tgattttccc gataaggtgt
    11461 cattccgtcg tataccacca ctccattacg gtttgttgtg cgatattttt gcccattgac
    11521 ataagcatct ttaattcctg gcgcattcat tacagcaaac gtttcggaaa gacggttcgc
    11581 cagattaacg ccacccgacc aggcgacaat gccccctgaa acactggctc cagcctgtcg
    11641 ataagtactc gactgactat aactgccatt caccgttgcc accggcgcgt tccaggttaa
    11701 attcgctcca gctgtcgttt catttccctg atgctgataa ctcaggttga ccccatagtt
    11761 aaactgatcc cggcttccaa cggttcctga taatcccgta ttatttgagg caaccccctg
    11821 atcatcaaac gtcgttgagt tagacatata tatttgccga cgaggcgtcg taacgtcatc
    11881 accccaatca aagggaatcg atataaaaat attaaaacgt ttctcttcat gatgattctc
    11941 gtcataagca tggcttgccg cgagggtata gcttatcctt cgcaggttgt tggaataact
    12001 caactgataa tccttgctgc tgccgctacg cccccagtaa tctcgccata acgtacttaa
    12061 ggacacagaa ccccagcctt ctggcaatga ctggctcata ttggcagaaa agctattttt
    12121 acggccaaaa tcgttctggt aataatcggc aatgtcatag atatcgtttt catcacggcg
    12181 ataattatct ttattgtttg cccatacgtg atcattaaat gtccgataat cacgcgacga
    12241 ataacgccag gccgccagac caaaacgtgt cgacgtttgg ctcacaaatt tgttgtaggc
    12301 aatttgataa ctttgcccgt caaacacatc gccgttgtct tgtatactat gcgacttcgt
    12361 ggcatcgacg gaaatggcac caatgcgtgt attccagcca gtccccaaag tgaacgcgta
    12421 ataattattc gcgaccatcg atccgccata cagcgtcaat aagttattaa aaccatactg
    12481 ataacccgcc tgaacaaaat cactttgttt gctcgcccct tcaatatggc tacgacctgc
    12541 cgcaaaatca tatttcgaca cgccgggttg cagcatattc ggcactgctg cataaggcac
    12601 cagataggtg gttaccgaac cgtccgcctc tttcacgctg acatcaagat ctgctccacc
    12661 accggccaac tgcaaatctg taatcgcaaa cgggccagga ggaacctctt tctgataaac
    12721 cacaaaacca ttctgttcaa tagttaccag cgcgttactc tgagcaatcc cctgcacccg
    12781 tggagtaaaa ttttgtttcg agttaggcaa catctgcata tcacgaaaca accgcacacc
    12841 gctgaagcga acagaatcaa aaatatcgct tgatgtgtac atatcaccca cgcgaagcgt
    12901 gccgagaagt tgggcaaatc cacgttccag atacagggta ttgcttttcc acacccctgg
    12961 attgctattt gttttactga aactggcatc agaatgcagt tgccacccca gtaagtttaa
    13021 cccgctgtta aaacgtacat atgtactctt gctattaccc gacgctttat agtcgctgta
    13081 atactgactc acataataag aggtataaaa cgcattaata ccccgctccc agttttccgg
    13141 tggaacatag ccactttcca gttcttccac ccacgcctgc gggacactga aatcgagacg
    13201 aaaaactccg atatcccagg tatagctccc accctgaaca agttgctcaa atgttaaaca
    13261 ttgcttaccg ctggcaaagt tatcggtatt aatgcctaac cgcttgataa tttcccttga
    13321 taaacatgtt tcttgcgggt tgtctttaac aataatctca tatttcccgc gccattgctt
    13381 attgacataa atatcgatgt catactgccc cggcaagggt tgattatcat caagacgaat
    13441 atttgcaacc tgctggtctt tcattccacc tatcatgaaa tgggtatcaa aggtttcttc
    13501 agctgcataa gcttcaatgc cgagcaataa cgctacgatt gctgatgcaa gtggggtcat
    13561 tctcaacata ggcagctcct gcaattaaat tttgtcacta atatagttgc catggtcatc
    13621 gataatggtc agatgccagt tattcgcatt attacttttg acattaatac tctgactttc
    13681 taagggggca atcataatag tttcataatt gactttgaca ttattagctt tgacatccga
    13741 aatcgttacc caattttctg agtcattttt tatcaccaat ccattgccac tgcgatttac
    13801 cagtaacttt ttaaatgtcg ctttatttac cggagcaata cccgctggcc ggtaaaacaa
    13861 cttaattctg ttttgcatcg caaacttcag tgcattctta ccttcttgtt ctggactatt
    13921 tggtggaata tccagaacat tcagataaaa aatactctct ttattagtgg gtagtttatt
    13981 cggcataatt ttaattttta cttgctgccc ggaatttgcc cctatttttg ccactggtgg
    14041 cgttaacatg aaaggaacct gaattttttc tggtggtaat gacgtatcgc catcatcaat
    14101 ccacgcctgc agcagcgaag aacggtttcc ctggttcatc aactgcacca tcacttcttt
    14161 attttctgcc gggtaaataa tgcgcgtccc gtagataaca ataccggcat gtgcaggaag
    14221 gaccatagaa gaaatgagta aagctaataa tcctttcatc ttaatgcctc ttaaattaaa
    14281 aggccgccat ggggatagcg gccatgttta ttacctggag aaacttgcaa ttttatttat
    14341 taatcctgcg ccacacgcat aaccagagaa gataacacat cacctgattg cactgaatta
    14401 tctgtcagct tcaggtattt tgctttaaac ggaatagcat attgaccttc cgctgtttta
    14461 taactctcaa tgttaaaagg tgcagaaata tccaggacat cactgttatc acctaaatag
    14521 agatagtaac ccacggtatc gttgctactg gctatcgcct tatcgttgcc ggttattttg
    14581 tcgtttgtga tcaacgagag ttttacacca gcaacttctt tcgggcagct aatttttaac
    14641 atgctgctgg ttacaccgac tgcatcatca taggtagaat atttagcttt accttcaaag
    14701 acagtggttg gtacagttgg catattaata cttactgaat cgccacctgc ttcgaattta
    14761 cacggagatt ctgcaatatt ccctttaatg gttaatgttc cggtatcaac gtctgcggca
    14821 aataccccag cgctcataaa aaaagaagaa aaaacagcgg cagcaataat tgaacgtttc
    14881 ataatatgct ccttaataaa atctattttt ggttgttaag agtctgtatc tcttggaaac
    14941 aaatttctca cacccaaaaa gttgccgcaa tgtaaacaca ttgttttatt gatttaaagc
    15001 aaattaacca ttgcaatata aacacaacaa atacttaaac gcattaaata tttatcaaat
    15061 ctattaatta atgtagattt ctataaatgg t
//
LOCUS       DAZGQL010000116        15090 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.116, whole genome shotgun sequence.
ACCESSION   DAZGQL010000116 DAZGQL010000000
VERSION     DAZGQL010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15090
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.116"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            111..1034
                     /gene="yicL"
                     /locus_tag="ACHKIR_004271"
     CDS             111..1034
                     /gene="yicL"
                     /locus_tag="ACHKIR_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate/amino acid/amine transporter"
                     /protein_id="HFO9991928.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLIGTFLLVTHGNPTSLSISPAALF
                     WGIASAFAAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYARQGTNFVVNGSLI
                     LAFFYLVVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARHE"
     gene            complement(1038..1856)
                     /gene="nlpA"
                     /locus_tag="ACHKIR_004272"
     CDS             complement(1038..1856)
                     /gene="nlpA"
                     /locus_tag="ACHKIR_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HFO9991929.1"
                     /translation="MKLTTHHLRAGAALLLAGILLAGCDQSSSDEKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVNDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSPEVAKAAETIFNGGAVPGW"
     gene            2078..2371
                     /gene="yicS"
                     /locus_tag="ACHKIR_004273"
     CDS             2078..2371
                     /gene="yicS"
                     /locus_tag="ACHKIR_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YicS"
                     /protein_id="HFO9991930.1"
                     /translation="MKPTMLLMITVFLIFPAISQAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            complement(2412..3602)
                     /gene="nepI"
                     /locus_tag="ACHKIR_004274"
     CDS             complement(2412..3602)
                     /gene="nepI"
                     /locus_tag="ACHKIR_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015211 - purine nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015860 - purine nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HFO9991931.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAAMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFVGTSLSSFIL
                     KRSVKLALAGAPFVLALSALVLTLWGSDKIVATGVAIIWGLTFALIPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(3813..4265)
                     /gene="yicN"
                     /locus_tag="ACHKIR_004275"
     CDS             complement(3813..4265)
                     /gene="yicN"
                     /locus_tag="ACHKIR_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HFO9991932.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LAAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(4318..5652)
                     /gene="adeQ"
                     /locus_tag="ACHKIR_004276"
                     /gene_synonym="yicO"
     CDS             complement(4318..5652)
                     /gene="adeQ"
                     /locus_tag="ACHKIR_004276"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HFO9991933.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAVGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISSVIGEVDLSGALSLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            5827..7593
                     /gene="adeD"
                     /locus_tag="ACHKIR_004277"
     CDS             5827..7593
                     /gene="adeD"
                     /locus_tag="ACHKIR_004277"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFO9991934.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     GGEISGPIVIKGRYIAGVGAEYADAPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGL
                     GGKELNTYIAAGIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTISRQP
                     VSASDFALQFTPGKRYRVIEVIHNELITHSRSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVIRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(7640..9031)
                     /gene="uhpT"
                     /locus_tag="ACHKIR_004278"
     CDS             complement(7640..9031)
                     /gene="uhpT"
                     /locus_tag="ACHKIR_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HFO9991935.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQSFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(9287..10606)
                     /gene="uhpC"
                     /locus_tag="ACHKIR_004279"
     CDS             complement(9287..10606)
                     /gene="uhpC"
                     /locus_tag="ACHKIR_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HFO9991936.1"
                     /translation="MLPFLKAPADAPLLTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVIAIAAGVSALLLLPFLNAQTPREA"
     gene            complement(10616..12118)
                     /gene="uhpB"
                     /locus_tag="ACHKIR_004280"
     CDS             complement(10616..12118)
                     /gene="uhpB"
                     /locus_tag="ACHKIR_004280"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HFO9991937.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFSLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQHDERLMLVIE
                     DDGSGLPPDSGQHGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(12118..12708)
                     /gene="uhpA"
                     /locus_tag="ACHKIR_004281"
     CDS             complement(12118..12708)
                     /gene="uhpA"
                     /locus_tag="ACHKIR_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HFO9991938.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(12781..13071)
                     /gene="ilvN"
                     /locus_tag="ACHKIR_004282"
     CDS             complement(12781..13071)
                     /gene="ilvN"
                     /locus_tag="ACHKIR_004282"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFO9991939.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(13075..14763)
                     /gene="ilvB"
                     /locus_tag="ACHKIR_004283"
     CDS             complement(13075..14763)
                     /gene="ilvB"
                     /locus_tag="ACHKIR_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HFO9991940.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIEAQPAVAEKAIAPAFSEESIRDAAAMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPLVEAQPRAEWHQLVADLQREFPCPIPKACDPLTHYGLINAVAACVDDSAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYKQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNETDPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(14869..14967)
                     /gene="ivbL"
                     /locus_tag="ACHKIR_004284"
     CDS             complement(14869..14967)
                     /gene="ivbL"
                     /locus_tag="ACHKIR_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HFO9991941.1"
                     /translation="MTTSMLNAKLLPTAPSAAVVVVRVVVVVGNAP"
BASE COUNT         3873 a         4154 c         3734 g         3329 t
ORIGIN      
        1 atttcctcca ttcactaaaa ctgattgatt actctactcc ctcaccgtca tcggtagtgg
       61 tatccttaac gtatattgat gaaaagacaa aacagtatgc agagggaaaa atgggttcca
      121 ccagaaaggg gatgctgaac gttctgattg ccgccgtgtt gtggggaagt tcaggggtct
      181 gcgcgcaata catcatggag caaagccaga tgtcgtcgca gtttttgact atgacgcgtt
      241 tgatattcgc cggtttgatt ctgctgacgc tatcatttgt tcatggtgat aaaatctttt
      301 ctattattaa caatcacaaa gatgccatta gcctactgat tttttccgtg gttggcgcgc
      361 taaccgtaca gctcactttt ttgctaacca tagaaaaatc gaacgcagcc acggcaacgg
      421 tgctgcaatt cctctcaccg actattatcg tcgcctggtt ctcactggtg cgtaaatccc
      481 gcccgggcat tctggttttc tgcgctattt tgacatcgct gatcgggact tttttattgg
      541 tgacacacgg taatccgacg tcattatcga tctctcctgc cgcgttattc tggggcattg
      601 cctcggcatt tgctgctgca ttctatacca cctatccctc aacgctgatt gcccgctatg
      661 gcacgttacc ggtcgtcggc tggagtatgc tgattggcgg tctgatcctg ctgccctttt
      721 atgccagaca agggacaaac tttgtcgtta acggcagttt gattctggcg tttttttatt
      781 tggtggtcat tggtacgtcc ctgacattta gtctgtacct gaaaggagca caattaattg
      841 gcggtccaaa agccagcatt ttaagctgtg cagaaccatt aagtagcgcg ctgctctctt
      901 tgctgttgct ggggatcacc ttcacattgc cggactggct gggaacgctg ctgattctgt
      961 catcggtgat tttgatttca atggattccc gccgccgcgc cagaaaaata aatcgtccgg
     1021 cgcggcatga gtgagaatta ccagccaggc accgcgcctc cgttaaagat ggtttctgcc
     1081 gctttagcga cttcgggtga ttgataagat tgcagaaatt ccttcacgtt ttctgcattc
     1141 ttattatctt cccgtgccac cagaatattc acatacggcg aatttttatc ttcaataaat
     1201 acgctgtcgt taaccggaga aagtcctgtt tgctggatgt aagtggtgct gataatcgct
     1261 acatccactt tcgggtcgtc cagtacacgt ggcaactgtg ccccttccag ctccattatc
     1321 tgcaaatggc gtggattatc ggttatatcc agcgcggtag gtagtaaccc ttttccctct
     1381 ttcagagtaa ttagcttctc tttttgcaac agtaaaagcg cgcgaccaag gttggtaggg
     1441 tcgttgggga tcgccactgt cgcgccttct tttatttgcg ccacggtttt gatttttttg
     1501 gaataacccg ccatggggaa gacaaacgta tttcccaccg ctaccagttt ataaccatgc
     1561 gcctgattat cttgttcaag aaagggtcga tgctggaaga cgttcgcatc aagctcgcca
     1621 tgatttgtcg catcgttcgg tagcaacgaa ccgctaaatc ctaccagttc aacatcgagg
     1681 ccatatttct cttttgccac ctttttagcg acttccgcga catcttgttc tgcgccatta
     1741 ataacgccca ctttaatgtg cttttcatcg ctgctgctct ggtcgcaacc tgccagcaga
     1801 attcctgcca gtaataatgc ggcccccgcc cgtagatgat gtgttgtcag tttcattttt
     1861 ttatcctttt attaatgatt acctgactat agcggtaagg tcgctgcagt ttaaaaaacg
     1921 aaacgctatc gataagaata aaaaggaata aaagtggaat ataaagtctg gcacttctta
     1981 ctgaccacgc aaacaccttt cgtacagcat gatgagagcg gcaaaagcaa actacactta
     2041 tgcttcattc gatgcacatt tgcagaaggt tgccgctatg aagccaacga tgctactcat
     2101 gattaccgta ttcctgattt ttccagccat ttctcaggcc gagtcgccat tcagttctct
     2161 acagtcggca aaagagaaaa ccacggtatt gcaagattta cgtaaaattt gcacgccaca
     2221 ggcgtcatta tcagatgaag cgtgggaaaa gttaatgttg tcagatgaaa acaataaaca
     2281 acatatacgc gaggccatcg tggcgatgga acgtaataat cagagcaatt attgggaagc
     2341 gttgggtaag gtagaatgcc cggatatgta agaacggcaa caaccactga ccaacgcttc
     2401 tttacctgat atcaggattt cttcattttc acctttgcag tcaccaacaa tgcagtcagc
     2461 aacatcaatg tgccggacaa catcagcggc gaagtcagac caatgttatc caacgcataa
     2521 ccgccgattg ccgcaccgca ggtattggca agctgaataa cggccacctg aatagatcca
     2581 gccttttctg cctgatcggc caatgagcga gtgatccacg ttgaccaacc aacgggaatt
     2641 aaagcaaagg tcagccccca gataatcgcc acaccagtag caacaatttt atcgcttccc
     2701 cacagcgtca gtaccagcgc gctcagggcg agcacaaacg gtgcgcccgc taaggccagt
     2761 ttcaccgaac gtttgagaat aaacgacgaa agcgatgtcc cgacaaagct ggcgatacca
     2821 aaactcagca gcaccagggt taagccatca acaccgaatc ccgccagatt catatacact
     2881 gggcgaatat aagtgaaaaa agcaaactgt ccggcaaagg acataaagat ggcgatcatc
     2941 ccggccatca ctcccggacg ttgtaataag cggaaagtat tttgtttctg atgcgaaggt
     3001 tcacctggca gcgaaggcag tgatttgata atccagaaaa tacacagcac acccatcgcc
     3061 gccgccgcat taaagacatt gcgccaaccg ataagctcgc ctaaaaaact gcccaacggc
     3121 gcggcaatca ccagcgcaat agaaaccgcg ccgaagatca ccgacagcgc cttcggcacc
     3181 gtacgcggcg gtaccagacg catggtcagc gacgccgaca tcgcccagaa cccgcccagc
     3241 gccagcccca gacaggcgcg accgattaac agcagactga atgagttggc aaaggaaacc
     3301 agcaaacagg aaagcgtcag caaaacggcg aacagaatga caacgtagcg gcggtcagtg
     3361 gcctgaatgg tctgggtaat aaacaaactg gcaaacatcg ccacaaaggc ggtcacggtc
     3421 accgattgcc cggcaacccc ttccgaaatg cctaaatcct gggccattgg cgtcaacaaa
     3481 ctgacaggca aaaactcaac gataatcaga caggcgacgc aaaacgccac cgagaaaacg
     3541 gctgaccagt tcggtcgggt gatggcatcc gcgccgcggt tttcagcaat aaattcactc
     3601 atggtgttac ccgtggcatg tttcagaggg aaagccgacc actttaacat tgaaatgtgt
     3661 gacgtattta acgtttttgc aacttttacg gtgttgcttg agctggagtt aagatcgaaa
     3721 cggaacaggg caaagattac cggatgcggc gtgaacgcct tatccaccct acaggtcagg
     3781 cgcggtctgt aggctgataa gacgaagcgg ttttaatgca gccagtgaac gccctcggca
     3841 ggctgaaaga acgcgttata gcgcatctga aaagcattaa tatcctgcat ttcaggttcg
     3901 gtttcgcgca gaaaacccag cgccagcctg acctgagcat cggtaatggg agcggcaagg
     3961 cgggcttttt gtaaaatccg atgccattgc agcaagttct gttcgctacc atcgacaatg
     4021 acaatttgcc agatgagcaa cacttgcgcg gcgttttgca ggtgcgactc atccggacga
     4081 tgcaaataac gtaaaaattc gtcaccggct gactttccca tttgatcgac catcgactcc
     4141 acgggtacga catcgatgtt cagccgatcg ctgagacggc gaatcgcttt tctggccccg
     4201 gatgtcagca cccgaaaacc aaccacaaac actaccgcca gcgtggcgag cattatccag
     4261 atcatgagtt ctcctgcgta atcagaactc atgatatctg gaaaccctcg ccagttacta
     4321 atccaccaga ataatcttca gtgcaaacag agctgcgacc accaccacac acaggttcag
     4381 atcgcgccag cgcccggtgc acactttcat gatgcagtac gacataaagc cgagtgcaat
     4441 cccttcggtg atcgagaaag taaagggcat catcaccgtg gtaataaacg caggtaccga
     4501 ttcggtaaaa tcatcccagt taacgcgcgc cagacttgag gtcatcaaca cgccaacaaa
     4561 gattaacgct ccggcggttg cgtaaggagg aaccatcgcc accagcggtg agaagaacat
     4621 aaccaacagg aacataacgc cgaccacaac cgcagtcagc cccgtgcgac caccgactgc
     4681 cacaccagaa gtactttcaa tataggcggt aacagacgag gtgccgataa acgcacccgc
     4741 caccgaactg acgctatcaa catacagcgc cttattcata ttagggaatt taccgttact
     4801 atctattaag ccagctttat cagttacacc aattaatgtt cctgatgaat caaataggtt
     4861 gatcagcata aaggagaaaa tgataccggc gagttcaagt gataacgcgc cgctcaaatc
     4921 cacttcacca atgacgctgc taatatcagg cggaatggaa tagacgccgc taaaatgaac
     4981 atcaccgaaa aataatccac agcaagacgt caccacaata gaaacaagca ccgcggcatg
     5041 aaaatgacgt gatgacaaca cggttataat aaaaaaccct aaaataccta acaacacgcc
     5101 gtgagaactt aaatcgccaa tcatcaccag ggtgtcttta ttggcgacaa taacgccagt
     5161 attttttaat cccattaagg cgataaataa tccgattccg ctggtgatac caatacgtaa
     5221 acttaatgga atgttggaga tcatccagta ccggatacga aacagcgtga gcagaaatag
     5281 cccaactgcg ccccagaata tcgcgcccat cccggtctgc caggagatgc ccatcgcccc
     5341 cacgaccacg aacgcaaaga aggcgttcag ccccattgcc ggagccagcg ccacgggtaa
     5401 gttagcaaat atccccatcg caatactgcc gataccggca atcaaacagg tggtaacaaa
     5461 caccactttc gggtccattt gtgccgcgcc gaggatttgc ggattcacaa aaacgatgta
     5521 taccatggtt aaaaaagtgg tcatccccgc aatcaattct gtgcggacgg tggtcccatg
     5581 ctgaggtagt ttaaataatc gcgaaagcgt ccctgattca ttactcacgt aatcggtatt
     5641 gtcattattc atttttttgt ccattggagg agatttaatc ccttgctttt gtaaaacaaa
     5701 tgacaacaac ggttcagtga taattatcac atttcaattg cacattaatg gatattcttt
     5761 aataatctcg cgacgtttct ttatgataaa taataatcaa attgataaaa tcaaaatgag
     5821 aaaaatatga ataattctat taaccataaa tttcatcaca ttagccgggc tgaataccag
     5881 gaattgttag ccgtttcccg tggagacgct gttgccgatt atattattga taatgtctct
     5941 attctcgacc tgatcaatgg cggagaaatt tccggcccaa ttgtgattaa aggacgttac
     6001 attgccggtg ttggcgcaga atacgctgat gctccggctt tgcagcggat tgatgcccgc
     6061 ggcgcaacgg cggtgccagg gtttattgat gcgcacctgc atattgaatc cagcatgatg
     6121 acgccggtca cctttgaaac cgctaccctg ccgcgcggcc tgacgaccgt tatttgcgac
     6181 cctcatgaaa tcgtcaacgt gatgggcgaa gccggtttcg cctggtttgc ccgctgtgcc
     6241 gaacaggcaa ggcaaaacca gtacttacag gtcagctctt gcgtacccgc cctggaaggc
     6301 tgcgatgtta acggtgccag ttttaccctt gaacagatgc tcgcctggcg ggaccatccg
     6361 caggttaccg gccttgcaga aatgatggac tatcctggcg taattagcgg gcagaatgcg
     6421 ctgctcgata aactggacgc atttcgccac ctgacgctgg acggtcactg cccgggtttg
     6481 ggtggtaaag aacttaacac ctatattgct gcgggtattg aaaactgcca cgaaagttat
     6541 cagctggaag aaggacgccg gaaattacaa ctcggtatgt cgttgatgat ccgcgaaggg
     6601 tccgcagccc gcaatctcaa cgcgctggca ccgttgatca acgaatttaa cagcccgcaa
     6661 tgcatgctct gtaccgatga ccgtaacccg tgggagatcg cccatgaagg acatatcgat
     6721 gccttaattc gccgcctgat cgaacaacac aatgtgccgc tgcatgtggc atatcgcgtc
     6781 gccagctggt cgacggcgcg ccactttggt ctgaatcacc tcggcttact ggcacccggt
     6841 aagcaggccg atatcgtcct gttgagcgat gcgcgtaagg tcacggtgca acaggtactg
     6901 gtgaaaggcg agccgattga tgcgcaaacc ttacaggcgg aagagtcggc gagactggca
     6961 caatccgccc cgccatatgg caataccatt tcccgccagc cagtttccgc cagcgacttt
     7021 gccctgcaat ttacccccgg aaaacgctat cgcgttattg aggtcatcca taacgaattg
     7081 attacccact cccgctccag cgtctacagc gaaaatggtt ttgatcgcga tgatgtgtgc
     7141 tttattgccg tacttgagcg ttacgggcaa cggctggctc cggcctgtgg tttgctcggc
     7201 ggctttggcc tgaatgaagg tgcgctggcg gcgacggtca gccatgacag ccataatatt
     7261 gtggtgatcg gtcgtagcgc agaagagatg gcgctggcgg tcaatcaggt gattcaggat
     7321 ggcggcgggc tgtgcgtgat ccgtaacggt caggtacaaa gtcatctacc gttgcccatt
     7381 gccgggctaa tgagcaccga cacggcgcag tcactggcgg agcaaattga cgccttgaaa
     7441 gccgccgccc gtgaatgcgg tccgttaccg gatgagccgt ttattcagat ggcgtttctt
     7501 tccctgccag tgatccccgc gctgaaacta accagtcagg ggctgtttga tggcgagaag
     7561 tttgccttca ctacactgga agtcacggaa taataaaaaa aagcccggcg tcatgccggg
     7621 caaaagtcac cagttaagtt tatgccactg tcaactgctg aatttttttc tcgcggcgga
     7681 ttttgcgttc ttccatcacc gccactatcg ccatcaggca gatacaacca atcgcggcga
     7741 tatccagcgc ggcgaaggtg cctgcccagc cggtaaggcc aaataccggc gtcccatcgg
     7801 caatcattcc cagacctaac ttggcaaagc tgtcaccaat caggtaagcg aaggtgcctt
     7861 taataccatc ggcagcgcca atcgcttttt taggtacaaa gccaacagca gccacaccaa
     7921 tcaacaattg cgggccaaag accaggaaac ccaacgcaaa gagagaagcc aggtagatat
     7981 attcgttgct ggcgtgttga tacacaccga gcgtggcgat aatcagcgcc agcgcgatac
     8041 aggccaccag gccacggcga ccgttcgcca ggtcagagag ccagccccac agcagcgtac
     8101 cgaccagcgc accagcttca aacagcgtaa agctctgaat cgccaccgct ttagagagtt
     8161 tcagttcctg gaacgcatac acggttgacc actggtcgat accaatacgc accacataga
     8221 ggaaaatgtt ggcgaagcac agcagccaga tcactttgtt tttcagcaca tactcaacaa
     8281 agatctgcca cttggtcata tcggtagatt ctgtctcttt gtcctcttcg ctgatctcct
     8341 cgccgaacag ctcttcagct ttgccgaggc cataagattc cggggagtcg ctgccgtaac
     8401 gcaggccgat aaaaccgaca atcagcgcga taatcgacgg gaaaataaac atgccgatga
     8461 catgaccatc gaacaggtaa tttgcgccga acagcgccac acctgctgcg cctgcaccac
     8521 caaggttgtg agaaatgttc cagaaaccga ggaatgtccc gcgtttacga cgcggcgtcc
     8581 atttggtgat ggtggagtaa ctgcacgaac cgccggtact ctggaaaaag ccgctcaagg
     8641 cgtagaaggc aatcatcagg aacaggctaa ccgagccgct gcccatactg gcactgaagc
     8701 ccagcataca aatggcggac aggatcagca tgaacggcag gaattgtttg gtgtttttgc
     8761 cgtcggcgta gtaagaaacc agcgttttac ccacgccata agtgatggag aagcccaggc
     8821 cgatcatccc cagctgcgtc atgctcaacc cgtaggtcga gatcatgtcg ttctgcgcga
     8881 tgttaaagtt cttgcgaatc aggtacatcg taaggtagcc gataaagacc accaggtagg
     8941 attgcatgaa cggtttgaac cacattttgc gccgcacttc gagcggaagg tccagggtcg
     9001 gcttgcgaac ctggtttagg aaagccagca tgggttactc ctgatttccc ttcatacttc
     9061 aagccgcagg tgccttagct gcacgcattc ctcccggtca catagttcac tatgctcccg
     9121 ggactcacgc gtttgctgcg ttcctgcaac ttgaattatt ttgggaaaga attaatgaat
     9181 gatggcattg taaaaatcag cagcaaatca ggcctgagat agcgtccagg caaaacctgg
     9241 aaaattgctt agttttgccg gatatgaagt gaaaaggtga tatgcatcac gcttcgcgcg
     9301 gtgtctgggc gttcaaaaag ggcagtaaca gcagtgcgga aaccccggcg gcaatggcga
     9361 taaccacaaa aaatccgctc cagtgccagg tatcgagcac tttcgccagc ggccaaccag
     9421 caagcgacgc ccccagataa gcaaacaagc cgacaaaccc cgtcgccgcc cctgccgcct
     9481 ctttgtggga acattctgcc gccgccatgc cgattaacat ctgcgggcca aagacaaaaa
     9541 aaccaatggt gaagaagcag gttgcctgca tcacgtagct ggcaaatggc atcagccaca
     9601 gcgagccgac tgaaagcaaa attccggcgg cgaaaatcaa attcatcggc ccgcggttgc
     9661 cgttaaacaa tttgtccgag ccccaacccg ctaccagcgc accgataaat ccacccagtt
     9721 caaacatcgt cactgccgta ttcgccgtga ccagatcgac gcccagcgtc tcggacatat
     9781 acaaattgcc ccagtcgttg attgccgccc ggaccacata gaccagcaca tagcaaaacg
     9841 aaagcagcca gatatacgga ttcagtaaca catatttggt gaggatctct ttacgcgtca
     9901 gccctgcccc ttcttgttgt tgagctatct ccagcgcgtc gtgccgccat tcaccaaccg
     9961 ccggtaaacc taacgcctgc gggcgatcgc gcagccgcca gcagagaaaa atccccacga
    10021 ctatcgccat acaacccgca atcatcatcc cggcacgcca gccgtaatgc agcgcgctcg
    10081 ctgccatcac aatgggaatg agtgcgccgc cgacgttatg cgccgtgttc cataatgccc
    10141 accaaccgcc gcgctcggta cgagaatacc aggcggttaa cagacgcgca cacaccggtg
    10201 aaccccagcc ctggaaaaag gcgttcaaca cccaaagcac ggcaaacgcc catagcgacg
    10261 tcgagaagcc aaacagaatg ttgataatgc ccgtggcaat aagccctatc cccataaaat
    10321 aacgggcatt tgagcgatcg ctgacaatgc cggagacaaa cttcgacacg ccgtaggtaa
    10381 tgtaaaacag ggtcgctaac aggccaatat cgctacggct gagcacgccg ttagcaagga
    10441 tttctggtac ggcggcgtta aaacttttac gcgtgaagta aaacagcgcg taacccagcc
    10501 agatggtcag cagaatatgc cgacgccagt agcgatagcg ggcatcaatt tcatgtttat
    10561 cagtcagtaa tggcgcatcg gcaggcgctt tcagaaacgg caacatcatc gaaccttaga
    10621 cataacgttg aggtagagaa acgctgacac gcgtgccgtg cagacaggaa atgtgtaatg
    10681 tgccacccag cgccgttacg cgctcgcgca ttccggtaag gccaaaaccg tgttgcccgg
    10741 aatctggcgg taaaccgctg ccatcgtctt caatcaccag cattaaccgc tcatcatgct
    10801 gccagccttg cagcgtgacc gcgctggcat cggcatgttt cacaatgttg ttcaaccctt
    10861 cctggcagac gcgaaatagc gtcacgcgct ggttttcgct taacgctgat tcatcgattc
    10921 gccattcgag atgactgaca atgccgcgcc cttccagctc catttcccgc atcagtgagc
    10981 ggatggcctg ctccagggtg agatcatcca gctggcgcgg acgtaaccgc cctaacaagc
    11041 ggcgcaccgc gtcgtaaacg cccagcgata gttgttcgat gagctgcccg ctctgtttca
    11101 cgctggcgtt atccgccgcg agccgctgga caatgcccgc ttgagttcga atagcagtaa
    11161 tggtctgacc gatatcatcg tgcagctcac gtgccacatc acggcgcacg ctctcttcgg
    11221 tttccagcaa ccgttcagcg agatgctgat tgcgcgccag ttccttttgc agcgactggt
    11281 taagttcacg caaccgctgg atgcctgcgc ccagtaacaa ccctgtcaga ctttgcacca
    11341 gcagcgagag caataaatcc accggatgat cgcgccaggt ttgactggcg atcagcgcga
    11401 tggcgttcat caacgtcgca attagcgccc cttgccagcc atagtgccag gccagcgcga
    11461 taatcggcag cgccaggcag aatggcgtaa agcgcgacag ttcgtccggc aatcccaact
    11521 ggagccagag actgataaca aacagcagca agtaccagac cagatgccgc ccacgccagt
    11581 tgatcggttg agaaactaac gacgggccga gcggcagcca ggtgttattg gcgagatagt
    11641 gccagaacac cagacatatc ggggccagcg tcaggccgcc agttaaagtc agcaacagcg
    11701 cattccacga ctctttgccg tgccaaagcc agggcagcga ctgcaacaac gccgccgccg
    11761 ttaatgccgc cccttgtagc agcaaggtgc gccagtcacg ttgatgtcga tagcgcgaga
    11821 tcagcgccac gggcagtaac gtcagtaaac taccgatcat caataaggag aaatgggtta
    11881 aaccgaccgc ctgcgttaac cagtaaatca gcagccactc cgcgcccagc aacaccggcc
    11941 agtatccgcg cgggcattgc agcattagcc ccagacgcag accaaacgga aataacagca
    12001 ccgccatatc agggcgctca accaggtgca ggctgatact ccacaggcaa aaccatgcgg
    12061 cagagaagat aaaaaagcag gcaataacgg taattaagcg ggagaacaac gtcttcatca
    12121 ccaaccatca aacatgcggc gcgccagttc aacgtcgtta ctgacgccca gtttttccat
    12181 cagattggcg cgatggacgt gtaccgtttt cggcgacaag cccagttcgg cggcaatctc
    12241 tttcaccgcc attccttgcg ccagtttttc tgccacctgg cgttcgcgtt tggttagcgg
    12301 gtcctgacga ccggatgcca gtttaatggc aatatccggc gtcagataac agccgcccgt
    12361 ggcaaccgta tgcaccgcag caatcagttc gtcagggcta caacgcttgg agagaaagcc
    12421 gcgcgccccc gcgttaagcg cctgctcaac cagcgccgga ctgtcatgaa cagagagcat
    12481 aatcgtcgcc atacctttcg gcagctggct tagcagctcc agaccggaga tatcgggcat
    12541 ggagatatcg caaatacaca cctgcacacc gcgccccggc aaccccgcca gcgcctcgcg
    12601 ccccgaacca aactcggcaa ctacctgcaa atcaggttcc agccccagca gctgcgcaaa
    12661 gccggagcgg acgataaggt gatcgtctat aagggcaacg gtgatcatga tcttgtcctg
    12721 gcgggtaata aaacgcgctt accttaacga taagcgcgat gttgttcaag ccttgagcgg
    12781 ttactgaaaa aacaccgcaa tcttgttaaa cattgtcgga tcggactgat tacgctgcac
    12841 tttcaccaca tcttccagct tatcgatttg gcttatcatc tgctccagac gctggtcgtc
    12901 attgaccagt agccagatat ggcttttgtc gctgtcctga atcggcaggc agagaatgcc
    12961 ttcaacgtta aaagcgcgac gggcaaaaag gccacaaacg tgggtcatta cgcccggatg
    13021 gttgcgaacg gtgagttcga gaatcacgtt gtcatgagtt gtgttttgca tggcttattc
    13081 ccccaccatt tcagtattcg ccgcacctgg cggcaccatc gggtacactt tttcttcggc
    13141 atcaatgcgc acgtggatca gcgccggacc agggcgattg atgatttcct gcaatgcagc
    13201 ctgcggatcg gtttcgttat tcaaatcaca ggtttcgagg ccgaatccgg cggcaatctg
    13261 cataaagttg atttttcccg gataggtagc ggcaaaaacg ccctgcttgt agaacagact
    13321 ctgttgctga tgtaccagcc ccagcgcttc gttgttcatc agaatgattt tgacatccag
    13381 ctgattttcg ctagcggtcg ccatctcctg aatattcatc atcaggctgc cgtcgccgga
    13441 gaaacacaac actttgcgat ccgggttcgc aagcgctgcg ccaatcgccg caggcaggcc
    13501 aaagcccatc gtgcccagcc caccggaggt cagccactgg cgtgggcgat tgagcggata
    13561 agcctgcgcc gtccacatct gatgctgacc cacgtcggtg gtgataatcg cgctgtcatc
    13621 gacacaggcg gcaacagcgt tgatcaggcc gtaatgcgtt aacggatcgc acgctttcgg
    13681 gattggacac gggaattcac gctgcaaatc cgccaccaac tggtgccact ctgcacgcgg
    13741 ttgcgcttcc accagcggga tcaactgcgc caacacgtca tcaacatccg cctgaattgc
    13801 cacatgcggt tgcttgattt tgcccagctc tgcgcggtcg atatcgacat gaataatttt
    13861 ggcattcggg cagaactgct cggttttgcc aatcgcccgg tcatcaaaac gcgcgccgag
    13921 aacaatcagc agatccgcct cctgcaaaat atagttggtg ctgcgcacgc cgtgcatccc
    13981 tagcataccc agcgacagcg gatgcgcttt tggcaacata cccagcgcca ttaatgtcat
    14041 tgtggtaggc agctgcgctt tctccgccag ttcacgcact ctcgcaggcg cattgatcac
    14101 accaccgccc agataaagca ccgggcgttt ggcagcatta atcattgccg ctgcgtcacg
    14161 aatgctttct tcgctaaagg cgggcgcgat ggctttttct gccaccgcgg gctgtgcttc
    14221 aatctcaaaa acggccgttt gcacatcctt aggaatgtct atccacaccg ggccagggcg
    14281 gcctgattgc gcaatacgga aggcatcgct catgacctgc gggagttctt cgatatgtct
    14341 gaccagatag ttgtgtttgg tgatggggat agagatgccg taggtgtcga cttcctggaa
    14401 ggcgtcggtg ccgatcatcg aggcgggaac ctgaccagtg atgcaaatca gcgggatgga
    14461 gtccagccgc gcatcggcaa tggcggtcac caggttagtc gcccccggtc cgctacaggc
    14521 catacagacc gccggtttac cgtcggtgcg cgccattccc tgagcgataa accccgcgcc
    14581 ctgttcatgg cgagccagaa tatggcggat ttgcgtgctt tggcttaagg catcgtaaac
    14641 aggcaggata gaaccgcccg gaatgcccgt cacaatctta atgccctgct gttccaggaa
    14701 atgaacgata aattctgcgc cggtaaagcg cttacgcgtc gatgttgtgc ccgaacttgc
    14761 catgctccag tccttttctt ctgggccgac tttccgggag gtgcttaaac gaaaaacccc
    14821 gcccggtttg cgccggcggg gttttggaat cgtgtgttgt tccagtccct acggcgcatt
    14881 gccgacgacc accaccacac gcacgacgac cactgcggcg gatggcgcag ttggtagtag
    14941 ttttgcgttg agcatggaag tagtcattgg ggaccttgtt ggttttgtgt ttaacaatat
    15001 ttatacaagc acagctttac aggggagaca atggaaaatt tttcagcaag ggaaaattga
    15061 ggggttgatc acgttttgta ctgaattgca
//
LOCUS       DAZGQL010000117        14760 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.117, whole genome shotgun sequence.
ACCESSION   DAZGQL010000117 DAZGQL010000000
VERSION     DAZGQL010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14760
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.117"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..179)
                     /gene="safA"
                     /locus_tag="ACHKIR_004285"
     CDS             complement(<1..179)
                     /gene="safA"
                     /locus_tag="ACHKIR_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HFO9991942.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLG
                     ISEMGRIARDLTHFI"
     gene            complement(427..2706)
                     /gene="ydeP"
                     /locus_tag="ACHKIR_004286"
     CDS             complement(427..2706)
                     /gene="ydeP"
                     /locus_tag="ACHKIR_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HFO9991943.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRQDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDDVSDCYKPLSWQQAFDEIGERLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTIGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFTPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLTVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            complement(3039..3953)
                     /gene="ydeQ"
                     /locus_tag="ACHKIR_004287"
     CDS             complement(3039..3953)
                     /gene="ydeQ"
                     /locus_tag="ACHKIR_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO9991944.1"
                     /translation="MDKTFSIKVLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGVAGGVVIKAGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNSVVMPTGGCTVDSRNVNVNLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGATTDSARQVFANTAPDATKASGVGVSLMRNGKILVTGENVSLGTVNKSKVPLG
                     LSATYGQTGNKVSAGTVQSVIGVTFIYE"
     gene            complement(4013..4516)
                     /gene="ydeR"
                     /locus_tag="ACHKIR_004288"
     CDS             complement(4013..4516)
                     /gene="ydeR"
                     /locus_tag="ACHKIR_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO9991945.1"
                     /translation="MKRLHKRFLLATFCALFTATLQAADVTITVNGRVVAKPCTIQTK
                     EANVNLGDLYTRNLQQPGSASGWHNITLSLTDCPVETSAVTAIVTGSTDNTGYYKNEG
                     TAENIQIELRDDQDATLKNGDSKTVIVDEITRNAQFPLKARAITVNGNASQGTIEALI
                     NVIYTWQ"
     gene            complement(4529..5059)
                     /gene="ydeS"
                     /locus_tag="ACHKIR_004289"
     CDS             complement(4529..5059)
                     /gene="ydeS"
                     /locus_tag="ACHKIR_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO9991946.1"
                     /translation="MKYNNVIFLGLCLGLTTYSALSADSVIKISGRVLDYGCTVSSDS
                     LNFTVDLQKNSARQFPTTGSTSPAVPFQITLSECSKGTTGVRVAFNGIEDAENNTLLK
                     LDEGSNTASGLGIEILDGNMRPVKLNDLHAGMQWIPLVPEQNNILPYSARLKSTQKSV
                     NPGLVRASATFTLEFQ"
     gene            complement(5073..7724)
                     /locus_tag="ACHKIR_004290"
     CDS             complement(5073..7724)
                     /locus_tag="ACHKIR_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO9991947.1"
                     /translation="MTAFRAAFKAYRMHQVLLLPRFARLTIALGLATAVFPVDAEYYF
                     NPRFLSNDLAESVDLSAFTKGHEAPPGTYRVDIYLNDEFMTSRDITFIADDNNADLIP
                     CLSTDLLVSLGIKKSALLDNKEHSAEKHVSDNSACTPLRDRLADASSEFNVGQQHLSL
                     SVPQIYVGRMARGYVSPDLWEEGINAGLLNYSFNGNSINNRSNHNAGKSNYAYLNLQS
                     GINIGSWRLRDNSTWSYNSGSSNSSDSNKWQHINTSAERDIIPLRSRLTVGDSYTDGD
                     IFDSVNFRGLKINSTEAMLPDSQHGFAPVIHGIARGTAQVSVKQNGYDVYQTTVPPGP
                     FTIDDINSAANGGDLQVTIKDADGSIQTLYVPYSSVPVLQRAGYTRYALAMGEYRSGN
                     NLQSSPKFIQGSLMHGLEGNWTPYGGMQIAEEYQAFNLGIGKDLGLFGAFSFDITQAN
                     TTLADDTRHSGQSVKSVYSKSFYQTGTNIQVAGYRYSTQGFYNLSDSAYSRMSGYTVK
                     PPTGDSNEQTLFIDYFNLFYSKRGQEQISISQQLGHYGTTFFSASRQSYWNTSRSDQQ
                     ISFGLNVPFGDITTSLNYSYSNNIWQNDRDHLLAFTLNVPFSHWMRTDSQSAFRNSNA
                     SYSMSNDLKGGMTNLSGVYGTLLPDNNLNYSVQVGNTQGGNTSSGTSGYSSLNYRGAY
                     GNTNVGYSRSGDSSQIYYGMSGGIIAHADGITFGQPLGDTMVLVKAPGADNVKIENQT
                     GIHTDWRGYAILPFATEYRENRVALNANSLADNVELDETVVTVIPTHGAIARATFNAQ
                     IGGKVLMTLKYGNKSVPFGAIVTHGENKNGSIVAENGQVYLTGLPQSGKLQVSWGKDK
                     NSNCIVEYKLPAVSPETLLNQQTAICR"
     gene            complement(7766..8476)
                     /locus_tag="ACHKIR_004291"
     CDS             complement(7766..8476)
                     /locus_tag="ACHKIR_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO9991948.1"
                     /translation="MQTTRPPYSVSFMATVLLLLLFACHSTVANAAVALGATRVIYPA
                     NQKQVLLPVTNNDPASVYLIQSWIENAGDQKDTQFVITPPLFSMQGKKENTLRIINAT
                     NHQLPGDRESLFWVNVKAIPAMEKDQKNENTLQLAIISRIKMFYRPTNLAMAPEEAPA
                     MLRFRRSGSKLTLINPTPYFITVTNMKAGNSNLPNTMVPPKGEVSVDIPHAASGDISF
                     QTINDYGALTPRIKATMQ"
     gene            complement(8839..9402)
                     /gene="fimA"
                     /locus_tag="ACHKIR_004292"
     CDS             complement(8839..9402)
                     /gene="fimA"
                     /locus_tag="ACHKIR_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFO9991949.1"
                     /translation="MKLKHVGMIVVSVLAMSSAAVSAAEGDESVTTTVNGGVIHFKGE
                     VVNAACAIDSESMNQTVELGQVRSSRLAKAGDLSSAVGFNIKLNDCDTNVSSNAAVAF
                     LGTTVTSNDDTLALQSSAAGSAQNVGIQILDRTGEVLILDGATFSAKTDLIDGTNILP
                     FQARYIALGQSVAGTANADATFKVQYL"
     gene            complement(9836..10102)
                     /locus_tag="ACHKIR_004293"
                     /pseudo
     CDS             complement(9836..10102)
                     /locus_tag="ACHKIR_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(10378..11694)
                     /gene="hipA"
                     /locus_tag="ACHKIR_004294"
     CDS             complement(10378..11694)
                     /gene="hipA"
                     /locus_tag="ACHKIR_004294"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
                     /protein_id="HFO9991950.1"
                     /translation="MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLS
                     LPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYHAKSRQPFDLLSEIGRDSVGAVT
                     LLPENETITRPIMAWEQLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
                     LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVP
                     DAEIIKAGNVRALAVERFDRRWNAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIA
                     RIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQAGGSYRLTPFYDII
                     SAFPVLGGAGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLKFPQVQMHEIL
                     SDFARMIPAALDNVKTSLPTDFPENVVTAVETNVLRLHRRLSREWY"
     gene            complement(11694..11960)
                     /gene="hipB"
                     /locus_tag="ACHKIR_004295"
     CDS             complement(11694..11960)
                     /gene="hipB"
                     /locus_tag="ACHKIR_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HipB"
                     /protein_id="HFO9991951.1"
                     /translation="MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISN
                     FENNPDNTTLTTFFKILQSLELSMTLCDTKNASPESTEQQDLEW"
     gene            complement(12153..14042)
                     /locus_tag="ACHKIR_004296"
                     /pseudo
     CDS             complement(12153..14042)
                     /locus_tag="ACHKIR_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244610.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter barrel domain-containing
                     lipoprotein"
     gene            complement(14192..>14760)
                     /locus_tag="ACHKIR_004297"
     CDS             complement(14192..>14760)
                     /locus_tag="ACHKIR_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416028.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HFO9991952.1"
                     /translation="LLEEMASRVPPGSWGVMPIFSDRMRFKTWYHAAPSFINLSIDPD
                     KCNKATLFRALEENAAIVSACNLQQIADFSNIHPTSLVFAGGGSKGKLWSQILADVSG
                     LPVNIPVVKEATALGCAIAAGVGAGIFSSMAETGERLVRWERTHTPDPEKHELYQDSR
                     DKWQAVYQDQLGLVDHGLTTSLWKAPGL"
BASE COUNT         3885 a         3539 c         3232 g         4104 t
ORIGIN      
        1 ataatgaaat gtgtaagatc ccttgcaatg cgacccattt ctgaaatacc cagctgatca
       61 attgccgaaa aaatggctat caacgtaagt gtcagtaata agacaaccac aattacagac
      121 atgatttctt tatagttgtc tctttgcgtg attttgtttt tcactgtggt cgcatgcatt
      181 tcaaatatgt ttatttagcg gataacgtta aaaatcgtta atcagtatgc ttaatataac
      241 aggctggaga ataggggaaa taggactggc gcttcagtac tgagcggagt ttcttacagc
      301 tgtaggcgga ggtatatatc ttataacgag cagtttaatt tagcacatgt gagtaaattg
      361 cgcgacaatg gtcaatgagg aattcttctg ctgtgcggat gacagcagaa gaaatgagga
      421 ggcgaattaa tttgatggtt ctaattcaac cggaatactt ttatagccag gaatgccact
      481 taatggatcg tggttatcaa gtgttagcat gtgattcgat tctggaaaat aggtcaccag
      541 tgagcggtca gccatagggt aaatgaccac ttttaatcta tccatgcggc gtgagctacg
      601 tttaccgtct ggcgtgagcg caataagatt aactctttcg ccgtttttta cacggcaaat
      661 tttagcttgt ttagcactca taaagaccac atctcgttga ccgaatacac ctcgatagcg
      721 atcatccata ccataaatcg tcgtgttgta ctgatcgtgg ctgcgtactg tcgccatgac
      781 cagtttactg ttaaacgctg aagagggatc ttctaacagc cctttactgg taatgaaatt
      841 agccttacct gacggcgtca tccagcgcct ttcagcagct gcatttatca ggtgaaaacc
      901 accgggatga cggatgcgct ggttatagtc ggcgaactct ggcagcacag cttcaatgtc
      961 attgcgaatg cgatcataat cttccaccag atactcccag gctaccacgc tctggggtag
     1021 tgctgcctgc gcgattcccg cgaccactgc acactctgat ttcagcatta caccggcggg
     1081 ttttaacacg ccacgcgagg catgaatcat cgacattgaa tcctcaacgg ttaccgcctg
     1141 cgcaccgctt ttttgcatgt caatctcgct gcgtcccaga accggcagaa tatagctatg
     1201 ccgtgcggtc agcagatgag agcggttaag cttagtggct acgtgtaccg tcagatctaa
     1261 ttgtgttaac ggtacagcgc ttgcttcccg atctggcatt gccagtgcaa agttgccccc
     1321 catgcagatc aatgctcgag cctgccccgt acatattgct tgcatgctgg caattgcagc
     1381 atgtccaggt gcatgaggtg gggtgaaacc atagcgctca cccaggcgag ccagaaactc
     1441 tgcagacggt ttctcggtga taccgacggt tcggtcgccc tgtacattag agtgacctcg
     1501 tagtgggcag atacccgcac caggcttacc aatgttacct ttcatcaaca gcagattgac
     1561 cagttgctgt acgttctggg taccatgttc gtgctgagtg atccccattc cgtaacagat
     1621 aatggttcgt tcggcagcgg catatgcatc agcaagttcg gcgatttgtg tctgacttag
     1681 tccagaaata cgttcgatat ctttccactc ggaattgagc acgtcacggc gtagctcgtc
     1741 aaagccgatg gtatgcgttt gaataaattc gtcatccagc aatgagggcc gacctgcggc
     1801 gcttgcagca tcatcgcgct caattaacag gcgcatcatc cccttgagca acgccatatc
     1861 accaccaatg cgcacgttgt agtaggcact ggccaactga gtctcagagt tcgtcagcat
     1921 ttcaaacggg ttttgcggtg cggtaaatcg ctccaggcca cgttcctgta gaggattgat
     1981 ggcgatcatt ttcgctcccc gtttcactaa agcgcgcaac gaagtcaaca tgcgagggtg
     2041 gtttgtacca gggttatgcc caatgcaaat gactaaatcg catttctcaa agtcttccag
     2101 caacacggtc cctttaccta caccgatact cgctgccaaa cccacactgg tcggttcatg
     2161 gcacatgttg gagcagtcgg ggaagttatt gctcccgtat tcacgggcaa aaagctgata
     2221 aagaaaggca gcttcattgg aagtgcggcc cgaagtatag aattcaacct gattgggatc
     2281 actatagctt tgaaggcgtt cgccaatttc gtcgaaagct tgttgccagc ttaatggctt
     2341 gtaacagtcg ctgacgtcat catatttcaa aggctgagtg agacgtcccg cagcctcaag
     2401 ctcgtgatct ccccaggtaa gtaatgattg aaccgtattc tcagcaaaga aagaggcatt
     2461 tacctgctta tccgtgactt cccaggcgat tgcttttgcg ccgttttcac aaatgtcgaa
     2521 tgacgcactg tgcttaggat ctggccatgc acatcccgga cagtcaaagc cctctggctt
     2581 attcatatca aacatggcaa taacatcctg gcgtatatcc atctgcttac gtactgcatt
     2641 tgctacggat ttaacagcac cccaaccacc ggcagctccc tggtaggatt caattttttt
     2701 cttcatctgc ttatcctgtg tggaaatgaa ttattaggat ttgtaatagc gatgatgcac
     2761 agtatataca aagggattat cgtcaacatg gatatataag agtaggggac tactctcttt
     2821 ctattttaat ctattatcgt taaacaaaag attaaatacg ataaattaag catcatctct
     2881 catagtgttt aaattaacat atttttcaat ataaaaaata agactctggc gttaattgtg
     2941 cgcggatttt cttacaggtg taggataata gccttcacaa aataattatc cgcgcaattt
     3001 tcgcgaatgg agcgcataga ctaaggtcaa aatcacaatt attcatagat aaaagtgaca
     3061 ccaatgactg actgaactgt tccggctgaa actttgtttc ctgtttgtcc ataggttgcg
     3121 cttaaaccca gaggtacttt gcttttattt actgtcccca gggagacgtt ttctcccgta
     3181 accagtattt ttccattacg catcagagag acacccactc ccgatgcttt tgttgcatct
     3241 ggtgctgtgt tagcaaacac ttgtctggca ctgtcagtgg tagcacccga aagataaaat
     3301 gagagctttt gttcacttga gcaatatacg ccaaggggta tttctgcgct tccaggaaag
     3361 tccgggagat tcacgttgac attacgacta tcaacggtgc agccgcctgt tggcataaca
     3421 acactgttat ttgaaataat attccacgta aaattacgcg gatttccgct tcctaacgtg
     3481 gcaattttat acatatgaat acgggcaata acttcccctg cttttatgac aactccaccg
     3541 gccactccga ctggggtaat atagagtttt aacggtaatg gcatcggtgt cttatcacca
     3601 atatccaata cgttggtatt tgtcgtcaac gggaaggggt aggtcacatt attccagtac
     3661 agtgatcctt tatatgactg tagggagcct gcgaaagctg atccttgtac caggtttata
     3721 tgatcagtgt cgtaccagcc gccgtaatca ttccagcaac ttatatgctg agacaagtct
     3781 acaaccagat tctggcccgg ttgtattaca ggatcgagat taacataaac agaggttgta
     3841 cctgctccaa tacttgaccc gccatcgaca ttacaactga aggcaaaaac tttgcctgcc
     3901 attaatagaa gataaatacc gaacaggacc tttatactga atgttttatc catgatatat
     3961 cctaaggtta aaaaattgat ttaaaaagga ggcaaaaaaa atcaaattga tgttattgcc
     4021 aggtatagat cacattgatt agcgcctcga tcgttccctg gcttgcgttt ccattcaccg
     4081 tgatagctct tgccttaagt ggaaactgtg cattacgagt gatctcatca acaataaccg
     4141 ttttgctatc gccatttttt aacgtcgcat cctggtcatc ccttagctct atctgaatat
     4201 tttcggcagt accttcattt ttgtaataac ccgtattgtc agttgaacct gtcacgattg
     4261 ccgtcactgc acttgtttca accggacaat cggttaatga caaagtaata ttgtgccagc
     4321 ctgatgcaga accaggttgt tgcagattgc gcgtataaag atccccgaga ttaacgttag
     4381 cttctttggt ttgaatagtg cagggtttag cgactacccg accattaaca gtgatagtga
     4441 catcggcggc ctggagagtt gctgtgaata acgcgcaaaa cgtcgctaac aggaacctct
     4501 tgtgaagtct tttcatatct ggctctgatt attgaaattc aagggtaaag gttgccgaag
     4561 ccctcaccag tcccggattg acggacttct gagttgactt cagacgagcg gagtaaggca
     4621 aaatattgtt ctgttctggt accagtggga tccactgcat cccggcatga aggtcattca
     4681 atttcaccgg acgcatattt ccgtccagta tttctatacc caaaccggag gccgtgttgc
     4741 ttccttcatc cagtttcaac agagtattat tttctgcatc ctcaataccg ttaaatgcaa
     4801 cccgaacccc cgttgtccct ttactgcatt cacttaacgt aatctgaaaa gggacggctg
     4861 gacttgtgct accggtcgtt ggaaattgtc tggcactgtt tttttggaga tctacggtaa
     4921 aattaagcga atccgatgag actgtgcagc cataatcgag aacgcgcccg ctaattttaa
     4981 taacgctatc tgcagataaa gcagaatagg tggttaaccc cagacataaa ccgaggaaaa
     5041 taacgttatt gtatttcata atctattgtt ccttagcgac agattgctgt ctgctggttc
     5101 agcaaggttt caggagagac tgcaggaagc ttgtactcga caatacagtt tgagttttta
     5161 tctttgcccc atgaaacctg taatttccct gactgtggaa gtccagtcag ataaacctga
     5221 ccgttttccg cgacaatgct gccattttta ttctccccat gtgtgacaat tgcaccgaat
     5281 ggaacgctct tattaccgta cttcaacgtc attaatactt tcccgccgat ttgtgcatta
     5341 aatgttgctc tggcaatagc accgtgagtt gggatgacag tgacaacggt ttcatccagt
     5401 tcaacattat ctgcaaggga attcgcgtta agagcgacac gattttctct atattctgtc
     5461 gcaaatggta atatggcata gccacgccag tcggtatgaa ttccggtctg gttctctatt
     5521 ttgacattat cagcgccagg agccttaacc agaaccattg tgtcgcccag cggctgtcca
     5581 aaggtgatgc catcagcatg agcaataatg ccaccactca ttccgtaata aatctggctg
     5641 ctgtcaccac tccgactgta accgacattc gtattaccgt aagctccacg ataattaaga
     5701 gaactgtaac cactggtgcc agacgatgta ttaccccctt gggtgttacc gacctgaacg
     5761 ctataattca ggttattatc cggcagcaga gtgccataaa cccctgatag gttggtcatg
     5821 ccgcctttca aatcgtttga catactgtaa ctggcgtttg aattacgaaa tgccgactga
     5881 ctgtctgtac gcatccaatg actgaaggga acattaagcg taaaagcaag taaatgatcc
     5941 cgatcgtttt gccatatatt attggaatag ctgtaattca gcgaagtcgt aatatcacca
     6001 aacggcacat ttaatccaaa tgatatttgc tggtcgctgc gtgacgtgtt ccagtaactt
     6061 tggcgactgg cactgaaaaa tgtcgtaccg taatgtccaa gctgctgaga gatgcttatt
     6121 tgttcctgac cacgcttact gtaaaacaga ttaaaataat caataaatag tgtctgctca
     6181 ttgctgtctc cggtaggagg cttgacggtg taaccactca ttcgactgta ggcactgtcg
     6241 gataagttat aaaaaccttg cgtagaatag cgatatcctg cgacctggat attggttccc
     6301 gtctggtaga aggatttgct gtagacggat ttaaccgatt gtccgctgtg acgggtgtca
     6361 tctgcaagcg tcgtattggc ctgcgtgata tcgaaagaaa aggcaccaaa aagtcctaaa
     6421 tctttaccaa taccaaggtt gaaggcctga tattcttctg caatttgcat tccgccataa
     6481 ggtgtccagt ttccttccag tccatgcatc aagctacctt gtatgaattt gggggagctt
     6541 tgcaggttat ttccactacg atattccccc atggcaagcg cataacgcgt atatccagca
     6601 cgttggagaa ccggaacaga cgaataagga acatataatg tctgaatact gccgtctgca
     6661 tcttttatgg ttacttgcag atcaccacca ttggccgcag agttgatatc atcaatagta
     6721 aaagggccgg gtgggacagt agtctgataa acatcgtatc cattctgttt tacactcact
     6781 tgtgcggtgc cgcgggcaat accatgaatc accggagcga aaccatgttg gctatcgggc
     6841 aacatcgctt ctgttgaatt tattttgagg ccacgaaagt ttacactatc aaaaatatcg
     6901 ccatcggtat aactatcacc taccgttaag cgtgagcgta agggaataat gtcacgttca
     6961 gccgacgtat tgatatgctg ccatttattg ctgtcagatg aattgctact cccactgtta
     7021 taactccacg ttgagttatc gcgcagtcgc caactaccaa tgttgatgcc actctgtaaa
     7081 ttcaaatatg catagttgga ttttcctgca ttatggttac tacgattatt aatagaatta
     7141 ccattaaaac tatagtttag tagcccagca tttattcctt cttcccacag atccggagaa
     7201 acatagccgc gagccattct gccaacataa atttgcggaa cggagagtga tagatgttgc
     7261 tgaccaacat taaactcaga tgatgcgtca gccaaacgat cccgaagtgg tgtgcatgcg
     7321 ctgttgtccg acacatgttt ttctgccgaa tgttccttat tgtctaataa tgcagatttt
     7381 tttatgccaa gactgacaag aagatctgta ctcaggcatg ggatgagatc tgcgttatta
     7441 tcatctgcaa taaaagtaat atcccgacta gtcataaatt cgtcgttcaa ataaatgtcg
     7501 acccgatatg taccaggtgg tgcctcgtgt cccttagtaa aggcggataa atcgacagat
     7561 tcagcaagat cattgcttaa aaaccgtgga ttaaaataat actctgcgtc aacgggaaaa
     7621 accgcagttg ctaagccaag ggcaatggta agccgtgcaa agcgaggcag tagtaacacc
     7681 tgatgcatac gatatgcctt aaatgcggca cgaaaggctg tcattattaa ttccgattga
     7741 gtttattatt aattaagtca ctgctttact gcatggttgc cttaatacgc ggagttaatg
     7801 caccgtaatc attgatcgtc tgaaaactga tatctccgga tgcggcatgc gggatatcca
     7861 ccgatacttc accttttggc ggcaccatgg tgtttgggag attgctattt cctgctttca
     7921 tattcgttac ggtaatgaag tagggcgttg gattaataag agtaagtttg ctaccgctgc
     7981 gacgaaaccg aagcattgca ggcgcttctt caggtgccat tgccaggttc gtggggcgat
     8041 aaaacatttt tatgcggcta ataattgcca gctgtaatgt attctcattc ttctggtctt
     8101 tttccatagc aggaatagct ttaacattca cccagaacag gctttcacgg tcgcctggta
     8161 actgatgatt agtggcgttg attatccgca gcgtgttctc ttttttacct tgcatggaaa
     8221 aaagcggtgg cgtaatgaca aactgggtgt ctttttggtc acccgcattt tctatccatg
     8281 actgaatgag atagacactg gcaggatcat tattcgtgac aggcaataaa acctgtttct
     8341 ggtttgccgg ataaattacc cgcgtcgcac ccagtgcgac agctgcgttg gcaacggtgc
     8401 tatggcaagc aaagagcaat aaaagtaaca ctgtcgccat aaaggaaaca gagtaggggg
     8461 gacgcgtagt ttgcatctga tgcattctcc atgtgttaag aggataagcg gggaaaccgt
     8521 tacatcagac aatcggtaaa tgttgctaat acattggcga gtcgccgggt ttccagactt
     8581 aatgcgtttg aaataaatgt tggttctctt ttaataggaa aaccttgcta taaagtaaat
     8641 attttaaata acagtaagtt taactactgt tgtttattga acgtttagat attatgtgat
     8701 tttttcatac cgctccaaaa cacaaactat tcctcattaa taatgagatg aacattggtg
     8761 aatttctcgg gtgtgcgtag ccatgccaca cacaccctgt ttcaatttca gatgacgtat
     8821 agccatctta agattgaatt atagatattg aactttgaag gtcgcatcag cgtttgcagt
     8881 accagctact gactgcccga gagcaatata acgagcctgg aacggcaata tattcgtgcc
     8941 atcaatcaag tcagttttag cgctaaaagt ggcaccatca agtattaata cctcccccgt
     9001 acggtccaaa atttgaatac cgacattttg ggcagagcct gccgctgaac tctgcagcgc
     9061 taacgtatcg tcattactgg tgacagtagt acccaggaat gcaacagctg cattactgga
     9121 aacattggta tcacaatcat tcagcttgat attgaagcca acggcggagc tgaggtcacc
     9181 cgctttagcc agacgagaag aacggacctg acccagctca accgtttggt tcattgattc
     9241 tgaatcaatc gcacaagcgg catttaccac ttcaccttta aaatgaataa caccgccatt
     9301 aacagtggtc gttactgatt catcaccctc ggctgcgctt accgcagcag acgacatcgc
     9361 caatacagaa acgacaatca taccgacatg tttgagtttc ataattctcc aaaatttata
     9421 tttaagtagc tgaataaaga gagttaacaa ttaagtcatt ctgttattgc ttataaaaca
     9481 agaatcattc ttaattggta gttgccctga gaatatagtt tccagggtta ttacatttca
     9541 cgaagaacga taatatcttt aaattacaag gacacctaat cacaacaagt ttattgcagc
     9601 acttgttgta gagagtattt gcaaagacat tttatgtcgc taaataacat gacgatttca
     9661 ttatgcatgt tatttataaa ccgtgactaa acagaatatt attgcgaaca agggaagtgt
     9721 caggaaaggt caagagcgac caatggttta tatggctata aatcattagc catagttaat
     9781 gtttgcctgg tgtaactaac acggaattgc tctaaacaga taagtgtagg gtttattaaa
     9841 gctgaccgac agactttctt aacctggtaa tatctctctt aggtgactct ccaaacatcc
     9901 gcgaatattc ccgactgaaa tgggataagc tttcgtaacc gacagcatag gctgcagtgg
     9961 tgacatcgta atgttcattc agcatcagtc gcctggcttc attgaggctt agccatttcc
    10021 gataccggag tggactcatt cccgcaagct gtctgaaatg ttgatggaaa gagggggtgt
    10081 tcatttgcac gcggtttgcc agcctgactc ttcaacgctg agaagatgcg gcaaacggca
    10141 aacctgcata ccagccagcc tatatccggt atgcacaggc caattatgaa agaggagagg
    10201 ctttatgttc tggaaccttg aagtaataac gtcgaccgga aaatcactct gcatcatcac
    10261 tcagattcaa tttaacataa tatacattat gcgcaccaac ataaaccaag ggagaatcca
    10321 gccgttggcg gtcatgattg tcctgctcat taacaacggc cacaccccat atcttactta
    10381 ataccattct cggcttaacc gacggtgcaa cctcaacaca ttggtttcaa ctgccgtcac
    10441 cacgttctcc ggaaaatctg tcggtaatga agtcttcacg ttatccagtg ctgccggaat
    10501 cattctggca aagtcactca ggatttcatg catctgcact tgcggaaatt tcagcacctt
    10561 tgctgtcgcc agaaaatgtc gcggataaat tttatcgatt gccgtttttt tgcctttgga
    10621 tgcgttaagc cccattgcca gtttgagatc gctgatgtgt attcctgcac cacccaatac
    10681 cggaaatgct gaaatgatgt cgtaaaatgg cgtgagtcga taactgccgc cagcctgaat
    10741 aaatacggag aagttttttg catggccatc cgttgcgcca atcaaccact ggaacacctg
    10801 aaacttcata aaatcatagc gatctttcag cgcctcgctg gaccccatca aaaaagccat
    10861 gatccgcgcg atgcctgggc ctccatctga ttcatatttc accgatgaag gtaaaccgaa
    10921 tgtctgacac atatcctcct gtggcaagcg aagtaaaacc gttcgctcag cattccaacg
    10981 cctgtcaaaa cgttcgaccg ctaacgcgcg cacatttccc gctttaatga tttctgcgtc
    11041 cggcacattc aacccaagtt ctttagccag cagcagacag taatactcat tatcaacgct
    11101 ttggctgaga tcgagcgtcg cattgggctg cctgatttcg ccaatcggta atttaatgat
    11161 gtgcgtcgtc ggcgttattc ctttcggaat gcaccagtca ttgcctattc tgagcagcgc
    11221 tgtcttctcc tgcgcgccag caaccgagat gcgaaagtca ttttcttctc taatcatgcc
    11281 cagagggata tctgctttat aagccgttaa tacttcttca agcctggctt cagtgagctg
    11341 ttcccatgcc attatcgggc gcgttatggt ttcattttcg ggtaataacg ttacggcacc
    11401 aacgctgtct cgccctattt ctgacaataa atcaaacggt tgtctggatt tagcatgata
    11461 acgtttaacg atccgatcac gtacaatcgg gctatcgggt aacaggttat cgaagaagtt
    11521 aaatacggca tcagaggtga tattccccct ctgcaatggc agcgaaagtg acaacggtct
    11581 ggcataacgg ctggctaacc actccggtgc atacttaaag gtgtgcgcgc cattggctaa
    11641 cttcgttaac tcgcctaccc gctggttgtt catccaagtg acaagtttag gcattaccac
    11701 tccagatctt gctgttctgt tgattctggc gaggcatttt tcgtgtcgca tagcgtcatt
    11761 gagagttcaa gcgactgtaa aatcttaaaa aaggtcgtca gcgtggtatt gtcagggttg
    11821 ttttcgaaat tggaaatcgt cgcctgctta ataccaattt ttttcgccag ctcgctctgc
    11881 gtccagccat tttgctggcg aaccagtttc attgcattcg ccaattgcgt tgggctatag
    11941 atcttctgaa agctcatcat gtcatacgtc cacagcaaat ttatccgctt aaggggatat
    12001 tatcagtttt atcctttagt gaggataagt caatctggaa acacatctta tccgctctac
    12061 gggataaaca ctgtgatatc cgcgatcgcg gataaaaagg aggatttaaa agtcgtttga
    12121 agtcggcaat attaagccgc atgcatcttc gacatgcggc tattcccttg ggtatcgcct
    12181 tttacgttca tcttatcggt gggcgaatta tcgccgccca gcgtcgcgtt gaacgtcatc
    12241 aggccattgt ttccggtata gttaccgtta acggtcagta cattccccgg tttcccccct
    12301 tcgctgttca tttgaaccgt gccaccattc tccaggttac cgccgacagt ggttttagcg
    12361 acacgcagtg tcccccctgg cataacgttc acatcacctg cagtggtgac atttcccgac
    12421 atcacaccgc tgttatggat attaatgtgc tgacttgcca tattaatggc agagtcggaa
    12481 ccgaaagcaa tttcaccacc cacgatatcc gtcgtaccct tatacgccgc ggaatctttg
    12541 ttaagcgtca ggatcccggt acccagtttt gtcacatcac cgctaccgct gatactgtgc
    12601 gtcatcaccc agctacgctc agtggagatg gtgagcaggc cattattgat aacggaagca
    12661 tcacccaggt ttttctgctc gttgacgcta agcgcgctgc ctgtatcaat gttgaattgc
    12721 cccacaaagc ggctattatc gccatccagc tctacgtctg tcctggcggt tgcgctcacg
    12781 atacccttcc cgcttatgct attacgcagt tcgccggtca cattttttag cgttaacaga
    12841 ccgtcattgg caatatttcc tctgcctaac ccctgcgtat tatcgaggct gacttccgca
    12901 tttgggctaa tgctggttaa cgcattgtag cgtgccttaa gcccctcgat agccagtgtt
    12961 ccccctgtaa cattcaggtt tccgccacct gtcagttcgc cctgactgat cccaccttta
    13021 ttcaccgtca gcgacccctc tttaaacaaa acagtcgaac ccatttgccc ggtgaatgtc
    13081 tctaccgtct gggtcgatcc attaagatcg acgatggccg cgttgctgat gttcagttcc
    13141 cgggtgttgc ccagcgcgcc atccgcatcg gtacgtaatg tccccatctg aacgtaggtt
    13201 gccccctgat agtcgttctg accgttggaa agcgaaacct gtcgtaccgt attgattgcc
    13261 agatcccctt cgccaccgat ttttgccgac atatcgaccg ttgcgccata agctccgcca
    13321 tgttcggcta acgtcagctt ttgcccacca tggatgttca gcgctttcag cccatagtta
    13381 acatacaaac catcaccagg tgcggtcgtg aggcggaatc cataatcacc ctcggcgacc
    13441 accgttccgt tttgcgcaat atgtaacgtt ttgtccgctt ccacctcgtc gccctgtaaa
    13501 tcacgtaacg ttaatgaacc acccgatcca ataaccgttt gcgccttcac cagttgaacg
    13561 ccgacatggc tatcgtcgtg ttccatcaaa ttgagcgtcg tcagagggtt attcgccatg
    13621 ggatcattcc acggtttagg cacgtcgata agcacgtcgc ccatcccgtt tacctgatag
    13681 ttacggcctt tccaggtgta gtcacccgcg ccgacaacca gcgtatcgac gctgatatat
    13741 ccctccgcaa gcgtcgcagc aggtatatcc gtatcgaacg tgagcgtacc gccattcatg
    13801 gctagtccac caatggattg ctctcccact tttaccgatg tggtattttc actgtcagac
    13861 tgcagcatcg cgtaagtaag cgcagcggtg ttgtcgcgtt ccagtgcgaa ggtactggct
    13921 ttcagttggg caacaccggc gaattcagtc cctgctgcat gggtaaagcc aaacattttg
    13981 tcggaggatg acagctgcac tcgcatcaag ccatcgcctt tgagcgcatt gatcagcgtg
    14041 aatagcaatt tccccttgaa tatagtactg gtgatggaac gatgaatctg caagctggca
    14101 tttaacaagc cagctctaaa agaagggaaa tgagaatacc aatactcaaa aactaacagc
    14161 cacggtcatc atggtgtggc tgtcaatgaa actataaccc aggcgctttc cataacgacg
    14221 tcgtcagtcc atgatcaacc agccccagct gatcctgata aactgcctgc cacttatcgc
    14281 gtgaatcctg ataaagttca tgcttttccg ggtctggtgt gtgcgtccgt tcccaacgaa
    14341 ccaagcgttc tccggtttct gccattgatg aaaaaattcc ggcaccgacg ccagctgcaa
    14401 tggcacatcc taatgcagtg gcttctttga ccaccggaat attgacgggt aatcccgaga
    14461 catcagcgag aatttgactc cataatttcc cttttgaacc tccgccagca aaaaccagcg
    14521 aggtgggatg aatattcgag aaatcagcaa tttgctgtaa gttacacgct gatacaatcg
    14581 ccgcattttc ttccagcgca cggaacaatg tcgctttgtt acatttatcc gggtcaatgg
    14641 acaagttaat aaaggaaggc gcagcgtgat accaggtttt aaagcgcatt ctgtcggaga
    14701 atatcggcat tacgccccac gacccaggcg gcacccgact ggccatctct tccagcagcg
//
LOCUS       DAZGQL010000118        14369 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.118, whole genome shotgun sequence.
ACCESSION   DAZGQL010000118 DAZGQL010000000
VERSION     DAZGQL010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14369
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.118"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(272..2557)
                     /locus_tag="ACHKIR_004298"
     CDS             complement(272..2557)
                     /locus_tag="ACHKIR_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFO9991953.1"
                     /translation="MAVKASGRFVPPSAFAAGTGKAITGAYAWNAPREAVGRERPLTR
                     DEMRQVQGVLSTINRLPYFLRSLFTSRYDYIRRNKSPVHGFYFLTSTFQRRLWPRIER
                     VNQRHEMNTDASLLFLAERDHYARLPGMNDKELKKFAARISSQLFMMYEELCDAWVDA
                     HGEKESLFTDEAQTHLYGHVAGAARAFNISPLYWKKYRKGQMTTRQAYSAIARLFNDE
                     WWISQLKGQRMRWHEALLIAVGEVNKDRSPYASKHAIRDVRARRQANLEFLKSCDLEN
                     RETGERIDLISKVMGSISNPEIRRMELMNTIAGIERYAAAEGDVGMFITLTAPSKYHP
                     TRQVGKGESKTVQLNHGWNDEAFNPKDAQRYLCRIWSLMRTAFKDNDLQVYGLRVVEP
                     HHDGTPHWHMMLFCNPRQRNQIIEIMRRYALKEDGDERGAARNRFQAKHLNRGGAAGY
                     IAKYISKNIDGYALDGQLDNDTGRPLKDTAAAVTAWASTWRIPQFKTVGLPTMGAYRE
                     LRKLPRGVSIADEFDERVEAARAAADSGDFALYISAQGGANVPRDCQTVRVARSPSDD
                     VNEYEEEVERVVGIYAPHLGARHIRITRTTDWRIVPKVPVVQPLTLKSGIAAPRSPVN
                     NCGKLTGGDTSLPAPTPSEHAAAVLNLVDDGVIEWNDPEVVRALRGALKYGLRTPNRQ
                     QRNGSPLKPHEIAPSARLTRSERMQITRIRVDLAQNGIRPQRWELEALARGATVNYDG
                     KKFTYPVADEWPGFSTVMARI"
     gene            complement(2547..2822)
                     /locus_tag="ACHKIR_004299"
     CDS             complement(2547..2822)
                     /locus_tag="ACHKIR_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5405 family protein"
                     /protein_id="HFO9991954.1"
                     /translation="MSIRIEIGERYVVTSDSFQFILHEKKRAESGKNAGQEWLAVVGY
                     YPKLSQLVSGLMHHDILTGSAKSFADLNAQVEQLSKRCSEAFGSYGR"
     gene            complement(2819..3043)
                     /locus_tag="ACHKIR_004300"
     CDS             complement(2819..3043)
                     /locus_tag="ACHKIR_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFO9991955.1"
                     /translation="MPDNIDFIQEQQAELLERQINAARVKHCGVSALVCEECDAPIPA
                     ARRAAYPSATRCVSCQSVFEAKNKHYRRMA"
     gene            complement(3043..3345)
                     /locus_tag="ACHKIR_004301"
     CDS             complement(3043..3345)
                     /locus_tag="ACHKIR_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5405 family protein"
                     /protein_id="HFO9991956.1"
                     /translation="MSCSHSVVLLNNALKIAVMENGDLSLIQLCLDKEKRDITESVIA
                     IYQNELNLLSDVVNLLVKRAVFHKQISSVDELTKLTTEIASYCADEFKKLNDKRSW"
     gene            complement(3345..3569)
                     /locus_tag="ACHKIR_004302"
     CDS             complement(3345..3569)
                     /locus_tag="ACHKIR_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2732 family protein"
                     /protein_id="HFO9991957.1"
                     /translation="MHTVSENQCGKYALLLQQARTEAQADAATRFSSHLDAMIRHITK
                     AELSRVEIVELLSQESEKFHNIGLSRGEVL"
     gene            complement(3633..4133)
                     /locus_tag="ACHKIR_004303"
     CDS             complement(3633..4133)
                     /locus_tag="ACHKIR_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017441883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein B"
                     /protein_id="HFO9991958.1"
                     /translation="MTVMTLNLVEKQPATMRRIIGKHLAVPRWQETCDYYNQMMERER
                     LTVCFHAQLKQRHATMRFEEMNDVERERLVCAIDELRGAFSKRRQVGASEYAYISFLT
                     VSQRRTLFMHARLTEKEFNQPYWRINEESCYWRDALFRALRELFSLFEYAPTILTSVK
                     PEQYLH"
     gene            complement(4130..4300)
                     /locus_tag="ACHKIR_004304"
     CDS             complement(4130..4300)
                     /locus_tag="ACHKIR_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9991959.1"
                     /translation="MNEPRCIAQLLRNESPRAIDFTITHGKGRKGIIIRTKKQSPLKK
                     ALTFLKSRRVWK"
     gene            complement(4311..4586)
                     /locus_tag="ACHKIR_004305"
     CDS             complement(4311..4586)
                     /locus_tag="ACHKIR_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory phage cox family protein"
                     /protein_id="HFO9991960.1"
                     /translation="MNRQLVSVTDAVPYQEFAKLIGKTPRAVRGMIEKGKLPVIEITD
                     PQSVSGRAGEYWVYLPAWNNGLKLAYESRPKEIRDGWLMWLGLGEPR"
     gene            4708..5007
                     /locus_tag="ACHKIR_004306"
     CDS             4708..5007
                     /locus_tag="ACHKIR_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000020919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO9991961.1"
                     /translation="MSIDVSEKLKLIRESERLNRKEFSELTGVAYSSLSSYESRSKNA
                     GVEAIMKVLQHPRFTKYTLWFMTDQVAPEAGQIAPALAHFGQNETTSPHSGQKTG"
     gene            5123..6136
                     /locus_tag="ACHKIR_004307"
     CDS             5123..6136
                     /locus_tag="ACHKIR_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFO9991962.1"
                     /translation="MAIKKLDDGRYEVDIRPTGRNGKRIRRKFDKKSEAVAFEKYTLY
                     NHHNKEWLSKPTDKRRLSELTQIWWDLKGKHEEHGKSNLGKIEIFTKITNDPCAFQIT
                     KSLISQYCATRRSQGIKPSSINRDLTCISGMFTALIEAELFFGEHPIRGTKRLKEEKP
                     ETGYLTQEEIALLLAALDGDNKKIAILCLSTGARWGEAARLKAENIIHNRVTFVKTKT
                     NKPRTVPISEAVAKMIADNKRGFLFPDADYPRFRRTMKAIKPDLPMGQATHALRHSFA
                     THFMINGGSIITLQRILGHTRIEQTMVYAHFAPEYLQDAISLNPLRGGTEAESVHTVS
                     TVE"
     gene            complement(6413..6730)
                     /gene="yegR"
                     /locus_tag="ACHKIR_004308"
     CDS             complement(6413..6730)
                     /gene="yegR"
                     /locus_tag="ACHKIR_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YegR"
                     /protein_id="HFO9991963.1"
                     /translation="MKKIAAISLISVFLMSGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAVHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            7145..8044
                     /gene="yegS"
                     /locus_tag="ACHKIR_004309"
     CDS             7145..8044
                     /gene="yegS"
                     /locus_tag="ACHKIR_004309"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HFO9991964.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEIIPTLVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            complement(8126..8899)
                     /locus_tag="ACHKIR_004310"
     CDS             complement(8126..8899)
                     /locus_tag="ACHKIR_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFO9991965.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSVSGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(9005..10045)
                     /gene="gatD"
                     /locus_tag="ACHKIR_004311"
     CDS             complement(9005..10045)
                     /gene="gatD"
                     /locus_tag="ACHKIR_004311"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFO9991966.1"
                     /translation="MKSVVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDVVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRNNVFALPADMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSREMSAP
                     QIQGVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFAQAVRDIA
                     RNAMPGKVLLIP"
     gene            complement(10093..11448)
                     /locus_tag="ACHKIR_004312"
     CDS             complement(10093..11448)
                     /locus_tag="ACHKIR_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015577 - galactitol transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015796 - galactitol transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HFO9991967.1"
                     /translation="MFSEVMRYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFIKQEKAVLA
                     E"
     gene            complement(11452..11736)
                     /gene="gatB"
                     /locus_tag="ACHKIR_004313"
     CDS             complement(11452..11736)
                     /gene="gatB"
                     /locus_tag="ACHKIR_004313"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HFO9991968.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            complement(11767..12219)
                     /gene="gatA"
                     /locus_tag="ACHKIR_004314"
     CDS             complement(11767..12219)
                     /gene="gatA"
                     /locus_tag="ACHKIR_004314"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HFO9991969.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPEIVETLITLPETQLKEYFTKYVLDSDE"
     gene            complement(12229..13491)
                     /gene="gatZ"
                     /locus_tag="ACHKIR_004315"
     CDS             complement(12229..13491)
                     /gene="gatZ"
                     /locus_tag="ACHKIR_004315"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HFO9991970.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFAIADKVGFARERIILGGDHLGPNCWQQENVDAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCAGDPIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITQVEDAANTLRTHQKAFIARGLAEALTRVIA
                     IVVQPGVEFDHSNIIHYQAQEAQALAQWIEKTKMVYEAHSTDYQTQTAYRELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMFDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLQGMEIPLGMISQYLPKQFERIQS
                     GELSAIPHQLIMDKIYDVLRAYRYGCAE"
     gene            complement(13520..14368)
                     /gene="gatY"
                     /locus_tag="ACHKIR_004316"
     CDS             complement(13520..14368)
                     /gene="gatY"
                     /locus_tag="ACHKIR_004316"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HFO9991971.1"
                     /translation="MVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAPVI
                     IAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVMID
                     ASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADALYTNPVQARE
                     FAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDIQQ
                     TIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIADC
                     GCEGRA"
BASE COUNT         3809 a         3706 c         3171 g         3683 t
ORIGIN      
        1 tcagccctga agcttcaaaa ctatcattta cgtttgcgag gcaccatcta tcacattgat
       61 ttataataag tatctttatc ttcactgcat tgctctcaaa aaatcacata gatggaaatt
      121 ccccgttgac acactcccaa gtgatgcaga tacatttcaa gtgcttcata cacatgataa
      181 cggctatccc cctaaattct gtgcgtttct tttccctcca gcacaaggtt gaaatatctt
      241 gcgtgcagct ttactttgtg aatctttgtc attatatccg cgccattact gttgagaatc
      301 ccggccactc atcagcgacc gggtacgtga attttttccc gtcataattt acggtcgcgc
      361 cacgcgccag cgcctcaagc tcccatcgct gcggcctgat accgttctga gcaaggtcaa
      421 cgcggatacg ggtaatttgc attctttccg accgggtcag tctggccgat ggtgcaattt
      481 catgtggttt taacgggctt ccgtttctct gctgacgatt tggggttctc agcccgtatt
      541 ttaatgcgcc cctgagcgcc ctcacgacct ccgggtcatt ccattcgata acaccgtcat
      601 caaccagatt aagcactgct gcggcgtgtt cagaaggtgt gggagccggt aacgaagtat
      661 caccaccggt gagctttcca cagttattga caggactccg aggcgcggcg atgccgcttt
      721 ttaaagtcaa aggctgaacg accggaactt tcggcacaat gcgccagtcc gtcgttctgg
      781 tgatacgaat atgacgcgcg ccgagatgcg gcgcgtaaat gccgaccact ctctcgacct
      841 cttcctcgta ctcgttaacg tcatccgagg ggctacgggc gaccctgaca gtctggcaat
      901 cgcgcgggac atttgcccca ccctgcgcgc tgatatacag cgcaaaatcg ccactgtctg
      961 cggcagcgcg tgcagcctcg acgcgttcgt caaactcatc agcaatgctg acgccgcgag
     1021 gcaatttgcg tagttcacgg taagccccca ttgtcggcag gccaaccgtt ttaaattgag
     1081 gaatgcgcca cgttgacgcc catgcggtaa cagccgcggc agtgtctttc agcggcctgc
     1141 cggtgtcgtt atcgagctga ccatccagtg catagccatc gatgtttttt gaaatgtatt
     1201 tcgcgatata tcccgcagca ccgccccggt taaggtgttt tgcctgaaaa cggtttcgcg
     1261 cggctcctct ttcgtcgcca tcctctttga gcgcgtagcg acgcatgatt tcgataatct
     1321 gattacgctg gcgtggatta caaaaaagca tcatatgcca gtgcggcgtt ccgtcgtggt
     1381 gtggctcgac gacacgcaaa ccgtagacct gtaaatcatt atccttgaat gccgtgcgca
     1441 tcaggctcca gatgcggcag agataacgct gcgcatcctt tggattaaat gcctcatcgt
     1501 tccagccgtg attaagctgc acggttttac tttcgccttt tccgacctga cgtgtcgggt
     1561 gatactttga cggtgcggtc agcgtgataa acatccccac atcgccctct gcggcggcgt
     1621 aacgctcaat accggcaatg gtgttcatca gctccatccg gcgaatttca ggattagaaa
     1681 tactgcccat caccttactg ataaggtcga tgcgctcgcc ggtttccctg ttttcgaggt
     1741 cacacgattt aagaaattcc agatttgcct ggcggcgcgc acgcacatca cgaatggcat
     1801 gtttactggc ataaggagaa cggtctttat tgacctcccc gacagcaatc agtaacgcct
     1861 catgccagcg catacgctgg cctttaagct gactaatcca ccactcatcg ttaaacagac
     1921 gggcaatggc agaatatgcc tgcctcgtgg tcatctgccc tttacggtat tttttccagt
     1981 agagcgggga aatattgaaa gcacgtgcag cgccagcgac atgaccatac aggtgagtct
     2041 gcgcctcatc cgtaaacagc gattcttttt cgccatgtgc atccacccag gcatcgcaga
     2101 gttcctcata catcatgaaa agctgcgatg agatacgggc ggcaaacttt ttcagctcct
     2161 tgtcattcat tccaggcagg cgcgcataat ggtcacgctc tgccagaaac agcaacgacg
     2221 cgtcggtgtt catttcatgg cgctgattca cgcgctcaat gcgcggccat aaacgacgct
     2281 gaaaagtgga tgtgaggaaa taaaacccgt gcaccgggct tttattgcgc cggatgtagt
     2341 cataacgtga agtaaacagc gagcgcaaaa agtaaggcag gcggttaatc gtggataaaa
     2401 caccttgcac ctgacgcatc tcgtcacgtg taaggggtct ttcgcgcccg acagcctcgc
     2461 gtggcgcgtt ccatgcataa gcaccggtaa tcgccttacc ggtgcctgcg gcaaatgctg
     2521 acggagggac aaaacgcccg gaggctttaa cggccatatg agccaaaagc ctcagaacaa
     2581 cgcttgctga gttgctcaac ctgcgcgttt aaatcagcaa aagactttgc gcttccggtc
     2641 agaatatcgt gatgcatcag gccggaaacg agctggctta atttcgggta ataaccaacc
     2701 accgccagcc attcctgccc ggcgttttta ccgctttccg ctctcttttt ctcgtggaga
     2761 ataaactgaa agctgtcact ggtaacgaca taacgttcgc caatttcgat acgaatactc
     2821 atgccattct ccggtaatgc ttgttttttg cttcaaagac tgactggcag gaaacacaac
     2881 gcgtggctga cggataagct gcacgacggg cagcaggtat tggcgcgtca cactcttcgc
     2941 aaaccagcgc agaaacaccg caatgtttta cccttgccgc gttaatctgg cgctccagta
     3001 attcagcctg ttgttcctga ataaaatcta tgttgtccgg cattaccagc tccttttgtc
     3061 gttcagcttc ttaaattcat cagcgcaata gctggcgatt tctgtcgtta attttgtcag
     3121 ttcatccacg gatgaaattt gcttgtggaa tacagcacgt ttaacaagta aattgaccac
     3181 atcagacagg aggtttaatt cattctgata aatcgcgata acagattcag ttatgtcgcg
     3241 cttttcttta tcaagacaaa gttgaataag agacaaatca ccattttcca taacggcgat
     3301 ttttaaggcg ttattcagta atacaactga atgagaacag gacatcaaag tacctccccg
     3361 cgagacaatc cgatattgtg aaatttttcc gactcctgac tgagcagctc gactatctcc
     3421 acgcgggata actccgcctt tgtgatgtgg cgaatcatgg cgtcaagatg agaagaaaag
     3481 cgcgtagctg catctgcctg tgcttcggtt ctggcctgtt gcagcagtaa tgcgtattta
     3541 ccgcactgat tttcagaaac tgtatgcatg actttctcca ggcaaaaaga agccccgcac
     3601 aattaagtgc gttaaaaact ctggttaatt acttaatgca gatattgctc tggttttacc
     3661 gatgtcagaa ttgtcggtgc atactcaaac agactgaata attcacgtaa tgcacggaat
     3721 aaagcatcac gccagtaaca tgattcttca ttaattcgcc agtacggctg gttgaattct
     3781 ttttctgtca gtcgtgcgtg cataaataaa gtacgacgct ggctgactgt taaaaaacta
     3841 atatatgcat actcacttgc accaacctga cggcgttttg agaatgcccc acgcaattca
     3901 tcaattgcac ataccagtcg ttcacgttcg acgtcgttca tttcttcaaa acgcatcgtt
     3961 gcgtgacgct gttttaactg cgcatgaaag caaaccgtta gccgttcgcg ttccatcatc
     4021 tgattataat aatcgcatgt ctcctgccag cgagggacgg ccagatgctt accaattatc
     4081 cggcgcatag ttgctggctg tttttcaacg agattgagcg tcatcactgt catttccata
     4141 ccctccggct tttcagaaag gtcagagcct tttttaacgg actctgtttt ttggtgcgga
     4201 taatgattcc cttacgcccc ttaccgtggg tgatggtgaa gtcaatcgcc ctggggcttt
     4261 cgttacgcaa taactgagca atacaacgag gctcattcat acggttctcc ttaacgtggt
     4321 tcaccgagac ctaaccacat caaccagccg tcacgaatct ctttaggacg gctttcataa
     4381 gccagtttta gtccgttatt ccatgccgga aggtataccc aatattcacc agcacgcccc
     4441 gatactgact gagggtcagt aatctcaata actggtaatt tccctttctc aatcataccc
     4501 cttacagctc ttggagtttt accaatgagt tttgcaaact cctgataagg cacggcatca
     4561 gtcacgctta caagctgtct attcatctgc tacgattctc ccttagtgct tctaatggct
     4621 cctaatggct aattattgcc taaaaggata actccagaag cacaacattt cacactatca
     4681 gcaagaaatt acgcaatcgg agtaattatg tcaatagacg tttcggagaa gttgaagcta
     4741 atccgtgaat ctgaaaggtt aaaccgtaaa gaattcagtg aattaactgg tgtagcctac
     4801 agctcacttt cgagctatga gagccggtca aaaaacgctg gagttgaagc cataatgaag
     4861 gtcttacaac atcctagatt tactaaatat actttgtggt tcatgactga tcaggtagct
     4921 ccagaagccg ggcaaattgc gcccgctctc gcacactttg ggcaaaacga aacaacgtcg
     4981 ccccactccg gtcaaaagac tggttaacaa tttatcgtga atatattcat cacaagtgcc
     5041 tactattggc ggctaaattt cagccaccac gaaaaaagcg attagtagta gcaaaaaaaa
     5101 gtaccactcg gagggttttc tgatggcaat caaaaaactc gatgatggtc gatatgaagt
     5161 ggacatccgc cctactggac gtaacggaaa acgcatccgt aggaagtttg ataagaaaag
     5221 cgaagctgtc gctttcgaaa aatacacgtt gtacaaccac cacaataaag aatggctatc
     5281 aaaaccaaca gacaaacgac gtctgtcgga actgacacag atctggtggg atttaaaggg
     5341 taaacacgaa gagcatggga aatctaatct tggaaaaatt gaaatcttca caaaaataac
     5401 gaatgaccca tgcgcatttc aaatcacgaa atcccttatc agccagtact gcgccacccg
     5461 aagaagtcag ggtattaaac cttcgagtat caatcgtgat ttaacatgta ttagcggcat
     5521 gtttacagcc ctgattgaag cggagttatt ctttggtgag caccctatca gagggacaaa
     5581 gaggcttaag gaggaaaaac cagaaacagg ctatctcaca caggaagaaa ttgccttact
     5641 gcttgctgct cttgacggcg acaacaaaaa gattgcgatt ctttgcctga gcactggagc
     5701 acgttgggga gaagcagctc gtttgaaagc agaaaatatc atccataacc gcgtcacgtt
     5761 tgttaaaacg aaaacaaaca aaccacgcac cgtcccgatc tcagaggctg ttgccaaaat
     5821 gatcgcggat aacaaacgag gttttttatt ccctgatgct gattaccctc gcttcagacg
     5881 aacaatgaaa gcaataaaac cggatttgcc aatggggcaa gccacacatg cactaaggca
     5941 cagctttgcc actcatttca tgattaatgg aggaagtatt atcacgctac aacggatact
     6001 aggtcacacg cggattgagc aaactatggt ttacgctcat tttgcgccag agtaccttca
     6061 ggacgccatt tctcttaatc cgctaagagg tggtactgag gccgagagtg tccacacagt
     6121 gtccacagta gagtaacgtt taagggcttt cagtggtaat ttatgccgct caaacccgca
     6181 ttgtaccgtt gaaagcccct actggtgaca ccctaaatct cccttacacg ggcttatttt
     6241 ttatacataa accatagaga cggattgatc ccaggtcacc gtcttccatt gaccacatcg
     6301 atagaatctc ccttcatagc acgatgcctt tcacttattg gcatcgtgct cgcacaggtt
     6361 ccggttacgc acagccagaa cgcgcatgtt tgatgcttac caaaaaatat tctcactctc
     6421 cacatttgaa tgtcagacga gcgacgccat gtaatcctgc accttctgtc ttcaggtcaa
     6481 ctatctgcat ttttttgccc tgagtaacac agaaatgaac tgcatcattt tttactatat
     6541 tttctgcacc agatattcta cccctggcta aagaagcttc ggcttcggtg tagtattggt
     6601 tatcgagttt acgctgaata ttacttttat atgcaagacc aaatttaccg atacttgtct
     6661 catcattatg tacagcacac ccagacataa gaaaaacact aattaatgat atagcagcta
     6721 tctttttcat ctcaccttcc cccattaaat accaacgaca cgctctggtg tttaaatata
     6781 ataatggcat gattattata attgaatagg attataataa atgttctgta caacatttcc
     6841 tacataagta ggaattacat acacggatgc ccttccaggt aaccccatgg gggggttaat
     6901 atatttttaa tagtatattg aagatgccac tgtttagttg aatattaggt atatgttctt
     6961 ttttgaaatt taccggtggc taccgttaat attcgctgtt cccattgcaa gctcctggtg
     7021 gtaaccactg aatcctccat acttgaactg actttttatc ctccgacttt catcctgttc
     7081 tgactccacc ttttcttttc tgctctacac tatctacagt ccaatcataa aggcacatac
     7141 gatcatggca gaatttcccg ccagcttact gattcttaat ggcaaaagta ctgacaatct
     7201 acccttgcgc gaagcaatta tgctgttgcg tgaggaagga atgacgatcc atgtgcgggt
     7261 cacctgggag aaaggcgatg ccgcacgata tgtagaggag gcacggaagt tgggcgtcgc
     7321 aacggtgatt gccggtggtg gcgatggcac cattaatgaa gtttctacgg cgttgattca
     7381 gtgtgagggg gatgacatac ccgcgctggg aattttacca ttaggaaccg ccaatgattt
     7441 tgccaccagt gtagggattc ctgaggcact ggataaggcg ctgaaactgg caattgccgg
     7501 taacgccatt gcgatagata tggcgcaggt caataaacaa acctgtttta ttaatatggc
     7561 gacaggcggg tttgggacgc gtattaccac agaaacgccg gaaaaattaa aagccgcgct
     7621 gggtggcgtc tcttacatca ttcatggctt aatgcgcatg gacactctgc aaccggaccg
     7681 ttgtgaaatc cgcggtgaaa actttcactg gcaaggtgac gccctggtca ttggtattgg
     7741 taacgggcgt caggccggtg gcggtcaaca attgtgcccg aacgcgttaa ttaacgatgg
     7801 cttgctgcaa ctgcgcattt ttaccggcga tgaaattatt ccgactctcg tatcaacctt
     7861 aaaatctgac gaagataacc cgaatattat cgaaggagct tcgtcgtggt ttgatataca
     7921 agccccacac gaaatcactt ttaatcttga tggcgaaccg ttgagtgggc aaaacttcca
     7981 tattgaaata cttccggcgg cgttgcgttg tcgattacca ccagattgtc cattgttgcg
     8041 ttaatcaaac tattctctgt tgaaccagag agaatccgta atgccggatg cggtgtaaac
     8101 accttatccg gcatacagaa caatactaca ccgtaattaa ttttacccgt gtcttctgca
     8161 atgccagttt atccacctca gacacagcat catcggtaat aataatgtca attttttcga
     8221 ttggcagtac ctgattaaaa ccgcgacgat taaatttcga tgaatcgaga acggcaatca
     8281 ctttattcgc cgctgtcacc atcgcaccgc taacggaata accttcatta aaggtcgtaa
     8341 taccattaac cgcatcaata ccatcagcac cgacaaacat taaatcggca ttaatatcct
     8401 gcaatgaacg ctcagcaatg gaaccatgca tcgagcgcgt tttatggcgc accgtaccac
     8461 cacaaaccac caaagtgatg tctttatttt cggaaagggc aaacgcagcc gggagactgt
     8521 tggtaatcac cgtgatattt ttggcggtca ttaatccttc ggcgataagc attgtcgtac
     8581 ttccgctgtc gagaataacg gtcatccctt cgtggatcat tttgactgcc gcctgcgcta
     8641 ttctgttttt cggcgcgctg gcgagctgaa atcgctcttt aaacccgact tcctgggtct
     8701 cggtttcgct attaccggac attattttcg ccgcaccgcc atgaaagcgc gtaaccacgc
     8761 ctttttgttc gagaaagcgc aaatcggcac ggattgtcgc ttccgaggca gcaaatactc
     8821 ccgccagatc ctgaacaagc acggttccct gttcattcac taattggatg atcttattcc
     8881 ttcgctcgaa tgaattcatc gtcatttacc catattcaat tgtggctagt gtaaacgaag
     8941 ggggaccatt ggtgaatgat ctgttttgca caataccggg cctgcatcac gctggcccgc
     9001 ggtttcaggg aatgagcaac actttgcccg gcatggcatt acgagcgatg tcacgcaccg
     9061 cctgggcgaa gctttcaaag cttccacggt gagcgattaa tggctccagg cttaacttac
     9121 gttctgtcag caaccggctc gccgtttccc actcctgccc cggccaaggg ctggagtagt
     9181 tcatccaact gccgataacc gtcagctctt tacgcaatat tttgccaaac gttgcagatg
     9241 ttaaatgcag atcctgatgc aacgtgccca ccagcgccag ttgggcatga ggaccggcaa
     9301 tctctaccgc cagttcgacg gtttgcggga cgccagccgt ctcgaggata agctgattaa
     9361 agcgcagctc gcgtaaaacg ccctgtattt gcggcgcgct catttcacgg ctgttaaatg
     9421 tttgcatcgc tccgaaagat tttgccagtg ccagtttttc tgagctaatg tcgatagccg
     9481 tcacactctt tgctcccagc gcgacagcgc actgaattgc cagcaggcca atggttccgg
     9541 caccaataat aataacgttt ttattctcac atccttgcgc taaatggaac gcatgcaggc
     9601 caacggtaat cggttcaata aaagcgccat cctcaatagg catatccgca ggtagtgcaa
     9661 agacattgtt tcgcttaacg acaatatatt cggcaaatcc accatcacgc cgcgagccaa
     9721 taaaatcata ttttgcgcac tgggaataaa accctttcaa acactctgga caagtaaaac
     9781 agggtaataa cggcacacag gcaaccacat cgccagggtg taaatcatca acaccggatc
     9841 ccaccgcatc aatatagccg ctaaattcat gacctaacgt tattggataa taatgtgcac
     9901 catttttaaa tatcctgggt aaatcggaac cacataagcc cgagctggca atttttaccc
     9961 gcacctcatc ctgatgttta atttcaggaa tgacgctttc tgcaacgcgc acgataccat
    10021 cagtatcatt caccactgat ttcataaaaa ctcctgatta ttaaggggga taacctcccc
    10081 ctcaggtaaa aattattctg cgagaacggc tttctcttgt ttaataaagc cacgcgctct
    10141 acgccaggtc atgaaaatac cggtaagata aatggcaccg ataataatga aaccgggaat
    10201 attttgcggg gagaaaacct gaatcagtaa ccaggtaatg ggagaaccgc cctgatccat
    10261 tgaagccacc atgcccccgg cttttaacgc cccagcatta gccgccagtt gggtgtgcaa
    10321 accaatagtt tgcgtcgcga tccacagggt gatgctcata atgatgacac ccgagattaa
    10381 ggtgcggaac agatttccac gatgcaccgc gaccgccatc gccacaaaga agccgatggt
    10441 ggcaaggtcg ccaaacggca gcacctgatt acccggcaca caaacagcaa ttaaaatggt
    10501 gagtggaata aaaatcaggc ttgccgatac caccgccgta tgtcccagca gcaacgccgg
    10561 atcaaggcca atcaggaact cctgaccgcc gaacttcgcc tgtaaacgac tacgggcctg
    10621 cttagcgatg ggcgttaaac catccatgat gggtttaatc acccgtggca tcagcagcat
    10681 aactgccgcc gtttttaccg ccagctgcaa tacacctttg acatcgtaac ccgcgaggat
    10741 gccgataatc agccccatca caaaacccac ggtgaccggt tcgccaaaag ggccaaattt
    10801 gcgctgaata tcatcggcgc taaatttaat tcggttaacg cctgggattt tctcgatgat
    10861 agcatcgacc agcaccgcaa tcggccccat atacgccgac gtaccgtgcg gaatagcaat
    10921 gccttccagc tcaaagaaat ttcgggtatc gcgggcaaac cagtcgccga gcttataaac
    10981 aaacgccgcg tgaattacca cacccgccat ccctatcatc catgaaccgg ttgccagatg
    11041 cagcaacgcg ccggtgaagg tcatatgcca gatattccag atatcaacat ttaccacccg
    11101 cgtcatacgg gtcagtaaca tcgccacgtt aaccagaatc gcaatcggaa tcgccaccag
    11161 cgcaatttgc gacgcccagg tcatgggtga agagcccggc cagccgacat cgaccacatg
    11221 cagattcagg tcgaaatttt ccgccatcgc tttcgccgcc gggccaatgg aatccagcat
    11281 taagccaatc acaaggccaa taccaacaaa gccaatcccg atatgcagac ctgctttaaa
    11341 gcaatcgcct gccttcatgc ctaatatttt agaaaaaata atgatgacaa tcggcagcat
    11401 caccgtaggg ccgaggtcga gaatataacg catgacttct gaaaacatag gtcacccctg
    11461 taagatagtc agaattttat tttgtaatgc ttcgataccg acaccagaaa caaaaggcat
    11521 gccgtgaact aacggaatat cgccaaaact tcgatccacc ctggcagtgg tgcatatcaa
    11581 atgcacgcca tccatatagg tttctatttc attaacccga cactggatta attcaacagg
    11641 aatattatga ctctgacaca actctttaat ttcttccgcc gccatcgtag aggtcgcaac
    11701 cgcgcctccg caagcgacaa taatcttgcg tttcataaat agtccttttt attgttacag
    11761 agggatttat tcgtctgaat ctaaaacata ctttgtgaag tattccttta actgggtttc
    11821 aggaagagtg attagtgtct cgacgatctc gggctgttgt aacttgccaa ataaacagcg
    11881 taaaagtttc aattgctgct gcggattttc cacaattaac gcaatcacca acgataccgc
    11941 cacgtcgtta tcatcatccg cttgctgaaa atgaacttta tttgttggcc ttaacagata
    12001 aatggctgac gacttagcat gaatcgcctc acaatgcggt attgcaatgg cgtgctgctc
    12061 aagcattatc ccggtaggga attctgcttc tctggcaatt aatgcctgtg gccaggtatc
    12121 atgaaccaca cctttcgcga gcatctcatt accgatatgg gttaaaactt cgctacgatc
    12181 gacaaaagaa attccgctac gaacaaacag gttagtcata taccgtcctt attccgcaca
    12241 gccgtagcga taggcgcgca aaacatcata aattttatcc ataatcaact gatgcggtat
    12301 tgctgataat tccccggact gaatgcgttc aaattgttta ggaagatact gactaatcat
    12361 gcccagaggg atttccatgc cttgcagatt caccatcatc gtttcgacgc tatttttaat
    12421 ccggctatgc ggccagtaat aacgaatacg atccgacagg ctgtaacgaa tatccagtaa
    12481 tgaatcgtta aaacccgtac gataatattt tttccagtat tgcggttcgt caaacatcac
    12541 ttcttcaatt accgccaggc aaccgctgcg attttctggg gcgataagtt cctgctcaat
    12601 ttgcgccagc gcaaatatcg cctcacgtaa agcaaaggtt aatgccggac cgactttcaa
    12661 aattgcaaaa tgatcgcgaa ctaattcccg ataggccgtc tgagtctggt aatcggtgga
    12721 atgcgcttca taaaccattt tggttttttc tatccattgc gccagcgcct gagcttcttg
    12781 cgcctgataa tgaataatat tgctatggtc aaattccaca cccggctgca ccacgatggc
    12841 aatcacgcgt gttaacgcct ctgccagacc gcgggcaata aacgcttttt gatgcgtgcg
    12901 taaagtattg gcggcatctt caacctgggt gatatgtact gactgaatgg cgctggcctc
    12961 accgcccgga accggaactt cggtgccaat gacatagctc agttgctcac gctggcaatc
    13021 tgtcgccaca ctttccgcag caaagcaaag cacagcagct cgttccgcaa ccgtttctgg
    13081 ggctaacggt ataggatccc ccgcgcaaga cattgacgca tcaagatgaa ttttactgaa
    13141 gccggcacga acatatgcct ttaccagttc gacggatttt tccatcgccg catccacatt
    13201 ttcttgctgc cagcagtttg gtcccagatg gtcgccaccg agaataatac gctcacgagc
    13261 aaatccgact ttatcggcaa tcgcaaaaac aaattcgcga aagtctgccg gtgtcattcc
    13321 ggtataaccg ccaaattgat tgacctggtt tgacgttgct tcaatcagca ctttacgcgt
    13381 gctgttgcga tcaaatgcca gcgccgcttc gataaccaac ggatgtgcag aacagactga
    13441 acatatgcca atatgttcac cagctttatg ccgggcaatt aacgttttca tgttttttcc
    13501 ttgttaaatg gcaggtgcgt tatgccctgc cctcgcagcc acaatcggca atcactttgc
    13561 tcaccacatc gcgcattgcg gatttagccg actgcaaata atcccgggga tcggtcgctt
    13621 cggggtgctc tgtcaggtaa ttttttaacg cctgcgagaa ggcatttttt agctctgttg
    13681 caacgttgat tttgcatatc cccagtttga tggtttgctg aatatcctta gttgataatc
    13741 ctgacgcgcc atgcagcacc agcggtaagt tcacccactg gcgaatgttc tccagtcgag
    13801 aaaaatcaag cgccggtgcg ctggcataca taccgtgagc cgtgccgatg gcgaccgcca
    13861 gggaatcaat tccggttgcc tcggcaaatt cacgcgcctg aacagggttg gtgtacaacg
    13921 catcggcttc attgacttgc acatcatctt cctggccgcc aagttgcccc agctccgctt
    13981 cgacgctgac atcaaagcga tggcaaaaat ccaccacctc tttgacccgt gaaatatttt
    14041 gcgcaaaagg caaatgcgag gcgtcaatca tgactgagcg cacgccagaa cgaaccttct
    14101 gagcgatatc gtcaaatttc gtgtgatggt cgagatgaat tgccagtgga tggtgatatt
    14161 gcttcgccat cgcgctgacc agcgccaaca gattttctgt accggcatga gtaaatgtgc
    14221 caggcgttcc ggcgatgatg accggcgcat gcaggttggc agcggtttct accaccactt
    14281 gcatcgtttc gagattgtga atattgaatg ccggaaccgc ataaccgccg cgctgtgcgt
    14341 tgttcagcat ctgctttgtc gataccacg
//
LOCUS       DAZGQL010000119        14285 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.119, whole genome shotgun sequence.
ACCESSION   DAZGQL010000119 DAZGQL010000000
VERSION     DAZGQL010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14285
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.119"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(252..445)
                     /locus_tag="ACHKIR_004317"
                     /pseudo
     CDS             complement(252..445)
                     /locus_tag="ACHKIR_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase zinc-finger binding
                     domain-containing protein"
     gene            complement(865..1047)
                     /locus_tag="ACHKIR_004318"
     CDS             complement(865..1047)
                     /locus_tag="ACHKIR_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9991972.1"
                     /translation="MLLAENPRQGMTTEARVVIRILLISDHVNRQDNTQIQTIMRIIY
                     IYLLQKMNITDKTKYK"
     gene            986..3073
                     /gene="iha"
                     /locus_tag="ACHKIR_004319"
     CDS             986..3073
                     /gene="iha"
                     /locus_tag="ACHKIR_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional siderophore receptor/adhesin Iha"
                     /protein_id="HFO9991973.1"
                     /translation="MRITTLASVVIPCLGFSASSMAAEDVMIVSASGYEKKLTNAAAS
                     VSVISQEELQSSQYHDLAEALRAVEGVDVESGTGKTGGLEISIRGMPASYTLILIDGV
                     RQGGSGDVTPNGFSAMNTGFMPPLAAIERIEVIRGPMSTLYGSDAMGGVVNIITRKNA
                     DKWLSSVNAGLNLQESNKWGNSSQFNFWSSGPLVDDSVSLQIRGSTQQRQGSSVTSLS
                     DTSATRIPYPTESQNYNLGARIDWKATEQDVLWFDMDTTRQRYDNQDGQLGSLTGGYD
                     HTLRYERNKISAGYDHTFTFGTWKSYLNWNETENKGRELVRSVLKSDKWGLAGKPREL
                     KESNLILNSLLLTPLGESHLLTVGGEYQSSSMKDGVVLASTGETFRQKSWSVFAEDEW
                     HLTDVLALTAGSRYEHHDQFGGHFSPRAYLVWDVTDAWTLKGGVTTGYKAPTMGQLHK
                     GISGVSGQGRTNLLGNPELKPEESVSYEAGVYYDNLSGLNANITGFITDFSNKIVSYS
                     IDDNTNSYTNSGKARLHGVEFAGTLPLWSEDVTLSLNYTWTQSEQRDGDNKGAPLSYT
                     PEHMVNAKLNWQITEEVASWLGARYRGKTPRFTRNYSSLSAVQKKVYDEKGEYLKAWT
                     VVDAGLSWKMTDALTLNAAVNNLLNKDYSDVSLYSAGKSSLYAGDYFQTGSSTTGYVI
                     PERNYWMSLNYQF"
     gene            complement(3291..3441)
                     /locus_tag="ACHKIR_004320"
                     /pseudo
     CDS             complement(3291..3441)
                     /locus_tag="ACHKIR_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066419.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            3935..4177
                     /locus_tag="ACHKIR_004321"
     CDS             3935..4177
                     /locus_tag="ACHKIR_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9991974.1"
                     /translation="MKDEIHSTSINPDVVEQFLALMNTHCKSSEEALAMLSTLCYNLM
                     VTCGIHSMDSQMLNGHGISMQLQGIDIAERPGKNIH"
     gene            4468..4836
                     /locus_tag="ACHKIR_004322"
     CDS             4468..4836
                     /locus_tag="ACHKIR_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001456887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9991975.1"
                     /translation="MKDNNTTATPEAWKMRFLANGTSGTWFYTDDIAVTDAWLRAHPD
                     DVEVIPLCRIPPRQHSHEQHIEELRAALARMLHMHHMMLETTHYRENITDARCIREMH
                     EAPLQALNILARYPHKKDEV"
     gene            4840..5052
                     /locus_tag="ACHKIR_004323"
     CDS             4840..5052
                     /locus_tag="ACHKIR_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9991976.1"
                     /translation="MWLLIFWLSSPFNVSSGDARQPTLAVHSQTFHTERACRDALRAI
                     KKENVGEFSLRGVCVSDGNTKEAESS"
     gene            5049..5321
                     /locus_tag="ACHKIR_004324"
     CDS             5049..5321
                     /locus_tag="ACHKIR_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neurotensin receptor R8"
                     /protein_id="HFO9991977.1"
                     /translation="MTDITDRHTLRRMSWSELTTAAQEAELNRDYERARIVWSFALHV
                     ATTTINKNLAIAHIRRCDTLLHKSKTVPGNNPRGRSVCLRPQHPRR"
     gene            complement(5630..6461)
                     /locus_tag="ACHKIR_004325"
                     /pseudo
     CDS             complement(5630..6461)
                     /locus_tag="ACHKIR_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555401.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            6519..6707
                     /locus_tag="ACHKIR_004326"
                     /pseudo
     CDS             6519..6707
                     /locus_tag="ACHKIR_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(6776..8002)
                     /locus_tag="ACHKIR_004327"
     CDS             complement(6776..8002)
                     /locus_tag="ACHKIR_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9991978.1"
                     /translation="MKDMQAMLPQTFYIKPGQFIWSYLGTFLFMLGGCIENSWLSAWL
                     NTQGFDQSQIGQIFAAYGIVVAVTSWLSGVCVDMFGPRKVMLAGFSVYLIASIAFLNF
                     AIPSHDFSTILISYMLRGVGYPLVCYSFLVRLTIQLDNHQQGIGTSLFWVVYNLGFTI
                     IGPVVAASLIPVLGHINVMWAGIGVALLGGIFMLVFERNAFILKPRTTSVFKELSAGI
                     SIMYQRPRIGLAVIVKTINGLGTYGFVVVLPLFLLDKQFTLEEWASIWGITFISNQIF
                     NIIFGWMGDKIGFRRTIQIFGSLLTGFATLIVYWVPIVWGHNYVAFMLAMCLWGAGLA
                     GFVPMTPLVPMMAPDKKGAANSAVNFGSGLGNFVGPALVSILTGFGTGVVMYTMAGLY
                     LFSGILVQFLKVPGEK"
     gene            complement(8075..9097)
                     /gene="iolG2"
                     /locus_tag="ACHKIR_004328"
     CDS             complement(8075..9097)
                     /gene="iolG2"
                     /locus_tag="ACHKIR_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-chiro-inositol-2-dehydrogenase IolG2"
                     /protein_id="HFO9991979.1"
                     /translation="MQKLRCGIIGLGRVGKMHVENMYLLPQLDIVCAADSFIEEMSDW
                     LSSVNITSGYKDYQEMLQRDDIEAVFIFTSTDMHEEIVTAAVQAGKHIFCEKPLSMRE
                     DEKASLAVLRAVKEKGVKLQIAFNRRFDPQFREVYELVRRGKIGQPQMIKITSRDPEL
                     LPHDLIKRIGGLIFDFTMHDFDMARFIMQDEVSEVYVKGNTLIDPTLKNIDDVDTLVV
                     MLTFRNGGYALIDNSRRAVYGYDQRVEVFGSAGMAYADNVSESAVKVYNSQNCIMKNP
                     LPDFALRYREAYRTEILHFIDSVLNHTPIVCTGEDALLAQRIAIAAKQSLKSGLPVKI
                     KTDIQL"
     gene            complement(9270..11210)
                     /gene="iolD"
                     /locus_tag="ACHKIR_004329"
     CDS             complement(9270..11210)
                     /gene="iolD"
                     /locus_tag="ACHKIR_004329"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_453702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016823 - hydrolase activity, acting on
                     acid carbon-carbon bonds, in ketonic substances [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HFO9991980.1"
                     /translation="MKTIRLTMAQALVRFLDNQYIEVDGREIKFVKGIFAIFGHGNVV
                     GLGQALEEECGQLSVHQGRNEQGMAHIATGFARQMRRQQIYACTSSVGPGAANMITAA
                     ATATANRIPLLLLPGDVYASRQPDPVLQQIEQEHDLTLSTNDAFRAVSRYWDRIIRPE
                     QLMSACISAMRVLTDPADTGAVTLCLPQDVQGEAWDYPDYFFARRVYRLERHVPTELM
                     LNDAVALILRHQRPIIICGGGVKYSQSEQELLRFAEQYHLPIAETQAGKGALSSAHPL
                     NVGGIGETGSLAANLLAQEADLIIGVGTRYTDFTTSSKWIFQNPDVRYLNINVSRFDV
                     FKLDGVQLQADARVALTQLCERLAPEGYISQWGKTIHRVRSQYLAEVDRVYAAEYSGK
                     DFKPEIVDHMDTQKVFEEFNEITHSWLTQTRVLGVLNRMLPENALVVAAAGSLPGDLQ
                     RIWQSRGDNDYHVEYGYSCMGYEVNAALGAKLAQPEREVYSFVGDGSFMMLHSELVTS
                     VQMGKKINVILLDNMTNGCINNLQMEHGMDSFFTEFRFHKQDNGRQEGGLIPVDFARI
                     AEGYGCKSYRVTSIGQLLEALEDARKQTVSTLIDIKVLPKTMAHKYLSWWRVGGARVS
                     RSERIQAVARMLDEHIGQARQY"
     gene            11612..13549
                     /gene="iolC"
                     /locus_tag="ACHKIR_004330"
     CDS             11612..13549
                     /gene="iolC"
                     /locus_tag="ACHKIR_004330"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_453701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047590 - 5-dehydro-2-deoxygluconokinase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HFO9991981.1"
                     /translation="MSMNNTVSSEQKTLDIICLGRVAVDLYGQQIGSRLEDMTTFAKY
                     LGGSSGNVAYGTAIQGLRSAMLARVGDEHMGRFIREELNRVGVNTQCLLTDPERLTAL
                     VILGIKDQDTFPLIFYRDNCADMALAPEDIHETFIASSRALAVTGTHLSHINTRAAVL
                     KALTYAQKHGLRRVLDIDYRPVLWGLTSLGDGETRFIASPQVTEQLQQVLHHFDLIVG
                     TEEEFHIAGGSTDTLTALRRVRQLTSAVLVCKCGALGCSVFEGNIADDWSQVQMHSGV
                     RVDILNVLGAGDAFMSGLLRGYLNDEGWEQACRYANACGALVVSRHGCAPAMPTKQEL
                     DDYLSREHAITRPDKDPHLNHLHRTTTRKQHWPELCVFAFDHRNQLVDIARATGASEF
                     TIPELKMLLLEGARQAAHEAGLLHNCGILADTTFGQKALNDVTGQGWWIGRPIEMPGS
                     YPLKLEHGNIGSQLVSWPKEHVVKCLVFYHPLDAESLRLKQEALIDEIYRACCQSGHD
                     LLLEVILPRDAADQSEHYYTQIVERFYLLGIQPDWWKLPPLSPARWQEISQHIERYDP
                     ECRGILILGLDAPESELKSGFAAAADIPWVKGFAVGRTIFGEPSRQWFSGQINDEQLI
                     AAVKQKYRTLIDYWREYRPAL"
BASE COUNT         3871 a         3584 c         3229 g         3601 t
ORIGIN      
        1 tccggagctc agtaacatta ccgggatggc gcaggaaaaa ggactggctg atgttatctg
       61 tcaacaggac gtcatttttc tgaaaaaata gctttttccc ggcaatctct gtttcaaacg
      121 gacggttaac aagaataacg taacagacaa tcatcacaca tactccctga ttagcataaa
      181 aacaacgata tcaaaagact agacgctttt tcaggaaaaa tcttatttgt ccaggattat
      241 tcttacgaga ccggcgcttc aacggtaccg tcacattttt tacaggcttt ttttacccgt
      301 tctgtgctga tcactttcag ggagctgctc accagttcca gttgttcagc gctgacttcc
      361 ccagataatc cagctcaccg ccacactccg ggcaacagct ttcttcaggc tccagccggt
      421 gtatttcacg gggaaggtgt gccggtgtca gcgctagtgg tataagacgg ttctccctca
      481 tcgtttgtcc ttagtattgt cagcgccaac aatgtgggct ggcattataa atgccgtcga
      541 ggcaatcgtt cgcctcccca gtgggcaatc caaatggtga ttgtcacggt tagacgttta
      601 ttttgaaggt cgactgaata aggtgataac gctgtagtat tttcacgtga cacagaattc
      661 tgaacgttct ctcttacaat atttcaatgt ttctgtccgt attcaccgga agaagtcatc
      721 gaccaaatca taccagtcgt ctcactccac tgaccattca tggttgacag gtggtgaaaa
      781 tccgcttctg cagtaaccat tttttattcg caaaaccgaa cacgccattg cggatggctg
      841 ttaactggca tgccgaggca gtcattattt atatttagtt ttgtcagtaa tattcatttt
      901 ttgtaaaagg taaatataaa ttattctcat tattgtttgt atttgtgtat tgtcttgccg
      961 attaacatga tcggagatta gtaatatgcg aataaccact ctggcttccg ttgtcattcc
     1021 ctgtctcggg ttttcagcca gcagcatggc agcagaggat gtgatgattg tctcggcatc
     1081 cggctatgag aaaaaactga ccaacgcagc cgccagtgtt tccgtaatca gtcaggagga
     1141 attgcagtcc agccagtacc acgatctggc ggaggcactg agagctgtgg agggtgtgga
     1201 tgttgaaagt ggtacgggta aaaccggtgg gctggaaatc agcatccgtg gaatgcccgc
     1261 cagttacacg ctgatactga ttgatggtgt ccgtcagggc gggagcgggg acgtgacgcc
     1321 taacggtttt tctgccatga atacagggtt catgcctcct ctggccgcga ttgagcgtat
     1381 tgaggttatc agggggccga tgtccacact gtatggctct gatgcgatgg gtggtgtggt
     1441 gaatatcatt accagaaaga atgcagacaa atggctctct tccgtcaacg ccgggctgaa
     1501 tctgcaggag agtaacaaat ggggcaacag cagccagttt aatttctgga gcagtggccc
     1561 tcttgtggat gattctgtca gtctgcagat ccgcggcagc acacagcagc gtcagggctc
     1621 atcggtcaca tcactgagcg acacatcagc cacgcgtatt ccttatccca cggagtcaca
     1681 gaattataat cttggtgcgc gtattgactg gaaggctacg gagcaggatg tgctctggtt
     1741 tgatatggat accacccgac agcgttacga taaccaggat ggtcaactgg gtagcctgac
     1801 agggggatat gaccatactc tgcgctatga gcgaaacaaa atttcggctg gctatgatca
     1861 tactttcacc ttcggtacct ggaaatcgta tctgaactgg aacgagacgg aaaataaagg
     1921 tcgtgagctt gtacgtagtg ttctgaagag cgataaatgg gggcttgccg gtaaaccgcg
     1981 ggagcttaag gaatctaacc ttatcctgaa ttcattgctg cttacccctc tgggagaatc
     2041 tcatctgctt actgtggggg gagagtatca gagttcgtcc atgaaagatg gggttgtcct
     2101 tgccagcaca ggtgaaactt tccgacagaa aagctggtca gtatttgctg aggatgagtg
     2161 gcatctcacg gatgtacttg cgctgactgc aggcagtcgc tatgaacatc atgatcaatt
     2221 cgggggacac ttcagtccgc gtgcatatct ggtctgggac gtgaccgatg cctggacgct
     2281 gaaaggcggt gtgaccacgg gatataaagc ccccaccatg ggacagttac ataaggggat
     2341 cagcggtgta tccgggcagg gacgtactaa tctactcggt aaccccgaac tgaagccgga
     2401 agagagtgta agttatgagg cgggggtata ttacgataac ctctccggtc tgaatgccaa
     2461 tataacaggc tttataacag acttctccaa caagattgtc tcttattcca ttgatgataa
     2521 caccaatagc tatacaaaca gcggaaaggc ccgcttgcat ggtgtggaat tcgccggcac
     2581 attgccgctg tggtcagagg atgtcactct gtcgttgaat tacacctgga cccaaagtga
     2641 acaacgtgat ggtgataaca aaggtgcacc gctgagttat acccctgaac acatggtgaa
     2701 tgcgaaactg aactggcaga tcaccgaaga ggtggcatca tggctgggtg cccgttatcg
     2761 cgggaaaaca ccacgtttca cccggaatta ttcgtcactg agcgctgtac agaagaaagt
     2821 gtatgatgag aaaggagaat acctgaaagc ctggacggtg gtggatgcag gtctgtcgtg
     2881 gaagatgacg gatgccctga cgctgaatgc tgcggtgaat aacctgctca acaaggatta
     2941 cagtgacgtg agcctgtaca gtgccggtaa gagttcgctg tatgccggtg attacttcca
     3001 gacgggatca tcaacaacag gatatgtgat acctgagcga aattactgga tgtcgctgaa
     3061 ctatcagttc tgataataac aaaacgctat cactgacggt agaatacgtt gccactgcaa
     3121 ctcctggcgg aacagtggca acgtattagg ttaagtgcat ttccgatccg ctaatgagat
     3181 ttcgttacca acaactaata tcgtcacagg aaatgcacgg attattttta acttatcatt
     3241 tacatacttg tccagagtgt aagcgcaccg tgaaggacgt ggggtaaaaa ttagtttaca
     3301 gagagagtga cgttccaggg caacaactct ttcatgcggt tggcaggcca ggtgttgatt
     3361 acactgatca cgtggcgtag ccacgtttcc ggctcgattc cgttaagttt tgcagctacc
     3421 gatcaggctg tacatcactg ccgcactatc gctcgtcatc tcaaagtccg gtctcgtcag
     3481 caggaaggta tcattctctc ccgccatttt tccgggggcc cggtcagata agtccctttg
     3541 tctatcgctg actcctggct cataacccgg ttagcagaat gcagggtcac cacctcgcca
     3601 cgaccgaatc cggataagtc aattgcacct tctcaatcgc cattttgtca caattaagtt
     3661 tgataaagca ggtcacctct gcactgaatc tgctccagtg cctcattggt ctccgtcccc
     3721 ccctttaaac tgccggataa gactgctgca cagcgccttc cccggaatca aatcactttc
     3781 cctgtccgcc acaatccgct tcatgctacc tcgtattttg tttcagagac cggaatctgc
     3841 cagaaagtga ctacgcgtgc acatatcggt tttctgggtt acatgcatcg tgttattttc
     3901 tgaaatgcaa aataataaaa aacaaggaca cataatgaag gatgaaatac acagtaccag
     3961 catcaacccg gatgttgttg agcagtttct ggcccttatg aacacacatt gtaaatcctc
     4021 agaagaggcg ctcgccatgt tgagtacgct ttgttacaac ctgatggtga catgtggcat
     4081 ccattccatg gacagccaga tgctcaatgg gcatgggata tccatgcaac ttcaggggat
     4141 tgatattgcc gaaaggccag gtaagaatat tcactaacct gaacagggcc tgaaaacgct
     4201 gtcttttcag gcctctgcag gcaaaaactg atgacaacaa aatttctgcg tttgtacccc
     4261 accgatgaaa aagacaaaaa ctcacaccgg gcttctagcc agtaaagata aaacccgacg
     4321 tgtcagcctc tacgaaacgc caacggcatg gtgtatcagg gggcaggagt gctacagcaa
     4381 aagtacagga cgccgctgtg gcagccacga cagtctgagc agactgcgcc tagacagcat
     4441 taagcctgtt gaatgagtgg tggcaccatg aaagataaca acacaaccgc caccccagaa
     4501 gcctggaaaa tgcgttttct ggccaacggg acatccggca catggtttta cactgacgac
     4561 attgcagtca ctgacgcgtg gcttcgcgcg cacccggatg atgtggaggt tatccctctc
     4621 tgccggatac caccccgtca gcattcccat gaacaacata ttgaagaact ccgcgcagcc
     4681 ctggcccgga tgctgcatat gcatcatatg atgctggaaa ccacgcatta cagggaaaac
     4741 atcaccgatg cccgttgtat ccgtgaaatg catgaagctc cattacaggc gctcaatatt
     4801 ctggccagat acccgcataa aaaggacgag gtgtgacgca tgtggctact gattttctgg
     4861 ctttcatctc cctttaatgt ctcttccggt gatgcccggc agcccacact ggcggtgcac
     4921 agccagacat ttcataccga acgggcctgt cgcgatgcat tgcgcgccat taaaaaggag
     4981 aatgttggcg aattttctct gcggggcgtt tgtgtttctg acggaaacac gaaggaggca
     5041 gagagctcat gacggatatt actgacagac atacacttcg ccgcatgtcc tggtcggaac
     5101 ttaccacggc agcgcaggaa gcagaattaa accgtgatta cgagcgtgcc agaatagtgt
     5161 ggtcattcgc cttacatgtt gcaacaacca cgattaacaa aaatctggcc atagctcata
     5221 ttcgacgctg cgatactttg ttgcataaat caaaaacagt acccggaaac aacccgagag
     5281 gccggtcagt gtgcctgaga cctcaacatc cccggagatg actgacattc cggactgacg
     5341 cagttgctgc atatctgtgg caatacgttg tatccagcca gacaggctat cccgcgtagt
     5401 gacaattaac ccgctttcct gcaccagctt cagccgggcc gtcggggatt ttaccagccg
     5461 gaaatatcag taaaacaaca tatcattatg ccatggtgta cgcggccagt gtatgcttac
     5521 ttaaggaatg agggataaat tggatattat tacttcactt tgctttttta tgagattcat
     5581 cacataagtg gggttttact gttaaataat ctgaatttat ttgatatccg taaagcagat
     5641 gtctcagttc actgtttcca acttttgtta ttcctctggg gcgaatgctg tttccgctgg
     5701 aatattcacc tggcaccagt cccagaaaag ccatgagctg ttcaggattg gaaaatcgcg
     5761 aaaaatcacc cagttcagca accagggtta ttgcgattag ctgaccaaca cctttaagag
     5821 cctgcaactg gcagaccaga ttgcacagag atcactcagg taacaggcga gatatttcct
     5881 gatcgagttg aagtatgcga tcttcaatct gctccagatt ctgacaataa tgttgaaagc
     5941 aatctgttga gatagcagac caaatgattg attaactaac caggttcgat gtttgccggt
     6001 tcagtgcttc ctggcataac gtttgtctgt tcgtgtcacg ttttgcagcg gcacgggctc
     6061 tgattaaatc ccgcatagct tcatgagtca gctcagggag ccagactggg ggaacctcac
     6121 catccctcag taacctggcg agagaaatta cgtcccgatg gtcatttttg atcctgtctg
     6181 ccggtgattt gggtatacgg gaagggggca ataaccaggc attttatatt catcactgtg
     6241 agttgatgat atagcccaaa cccacaggga ccaccttcat agcaggaaag aatgtttccg
     6301 taaggtccct gcaatttctg acataatcga tgtacagcca aggcttcgtt gttaatcgta
     6361 ccataataac gaacttcgcc aagccattca ggtgtagcaa tcgccaaggt ttctttatga
     6421 acatccagcc cgacgtaagc tgcaatattt tccgaatcca tgatgacctc tggagtatcc
     6481 caccaatgct atagtgagta tataaaggtc atatcatcta cgtgttcttc ggcaacgatc
     6541 acagtggaga gcgtggagcg ctactgtacg ggctgattgg cacctgccga ctgaacagta
     6601 tcgatccgga agcgtatctg cgccatattc tgagcgtact gccggaatgg ccttccaacc
     6661 gtgttgacga actgctgcca tggaacgtag ttctcaccga taaataagcg tcaatacggc
     6721 attcgattaa tgcacttaca gctaaatgga gtttttgaac aatatttgac gttatttatt
     6781 tctcaccagg aactttaaga aactgaacca ggatgccact aaataaatag agtcctgcca
     6841 tagtatacat aaccacgcct gtaccaaaac ctgtcagaat ggataccaac gcaggtccga
     6901 caaaatttcc caggccagaa ccgaaattca ccgctgagtt tgctgccccc ttcttatccg
     6961 gtgccatcat cggtaccagc ggcgtcatcg gcacaaatcc cgccagcccc gctccccaca
     7021 ggcacattgc cagcataaaa gcaacgtaat tatgccccca cactatcggc acccagtaga
     7081 caatcagcgt ggcaaacccg gtcagaagac tgccaaaaat ctggatagta cggcgaaaac
     7141 ctattttatc ccccatccag ccgaaaataa tgttgaaaat ctgattagaa ataaaggtta
     7201 taccccagat actcgcccac tcctcaaggg taaactgttt atccagcaaa aacagcggca
     7261 acaccactac aaaaccatag gtgccgagtc cattgattgt tttaacaatc actgccagac
     7321 caattcgggg tcgttgatac ataatggaaa tacctgccga caactcttta aagactgacg
     7381 tagttcttgg cttcagaatg aacgcattgc gctcaaagac cagcatgaaa atccccccaa
     7441 gcagtgcaac cccaatcccg gcccacataa cattaatatg cccaaggaca ggaattaacg
     7501 aagcagctac taccggacca atgatggtaa aacctaagtt atatactacc caaaacagcg
     7561 acgttccaat tccctgttga tgattatcga gttgaatggt cagtcggaca agaaaggaat
     7621 agcagaccaa cggataacct actccccgca gcatatagct aatgagaatt gtactaaaat
     7681 catggctggg aatcgcaaaa ttaagaaacg cgatagaagc gatcaggtaa acggagaaac
     7741 cagcaagcat aacctttctg gggccaaaca tatccacaca gacaccggat aaccaggaag
     7801 ttacggcaac cacaattccg taggcggcaa atatctgtcc gatctgagac tgatcaaatc
     7861 cctgagtatt cagccaggca gatagccaac tattttcaat acagccgccc aacataaata
     7921 aaaatgttcc caggtagctc cagataaact gacctggctt tatataaaat gtctgaggca
     7981 acattgcctg catatctttc attttataac cctcagtacg gataatctct cgctcgtaat
     8041 agtgcgagag acccttcgct atttacaaat gcaactaaag ttgaatatca gtttttatct
     8101 tcactggcag tccacttttt aatgattgtt ttgcagcaat ggcaatacgc tgcgccaata
     8161 atgcatcttc accagtacag acaatgggtg tatgattcag aacagaatca ataaagtgta
     8221 atatttccgt tctataagct tcacgatagc gtagcgcaaa atcaggcaaa ggatttttca
     8281 tgatacagtt ctgactgtta tataccttca ccgccgattc actgacattg tcggcatacg
     8341 ccatccctgc tgaaccaaag acctcaaccc gttggtcgta gccataaacc gcccgacgac
     8401 tattatcaat tagcgcataa ccaccgtttc tgaaggtcag catcaccacg agagtatcta
     8461 cgtcatcaat atttttcaga gtaggatcaa tcaatgtatt gcctttaaca taaacctcgc
     8521 taacttcatc ctgcataata aagcgagcca tatcaaagtc atgcatagta aaatcaaata
     8581 tgagtccacc tatcctcttg atcaaatcgt gcggcaacaa ctctggatca cgtgaggtga
     8641 tctttatcat ctgaggctga ccaatcttgc ctctgcgcac cagttcatac acctcccgaa
     8701 actggggatc aaaacgacga ttaaaagcaa tctgcagttt gactcctttc tctttaaccg
     8761 cccttagcac cgcaagggaa gctttctcat cttctctcat gctgagaggt ttttcgcaga
     8821 aaatatgttt acccgcctgg actgctgccg taacgatctc ttcgtgcata tctgtcgagg
     8881 tgaaaataaa caccgcctca atatcatcac gctgtaacat ttcctgataa tctttatatc
     8941 ctgaggttat atttaccgaa ctcagccagt cgctcatttc ctcaataaaa ctgtcagcag
     9001 cacagacaat atctaattga ggaagtaaat acatattctc aacatgcatt ttccctaccc
     9061 ggccaagacc aataatccca catcgaagct tttgcattta tacctcgtca tatcatgata
     9121 tagtaaatat aaaacgacaa aagaaaatcc cctttatcca acacggtacg aaagacttta
     9181 ttttgtagtt tcaacgaaca aatactttta ccgtaaaaaa cgtcaggttg cagaaaggcg
     9241 ccctcctgca accttaaaaa ctgaattaat tagtactgtc gcgcttgtcc aatatgctca
     9301 tcaagcatac gagctactgc ctggatgcgt tcgctacgcg acacgcgagc gccaccaaca
     9361 cgccaccagc tcagatattt atgcgccatt gttttaggca gtactttaat gtctatcaac
     9421 gtactcaccg tctgtttacg tgcatcctcc agagcttcaa gcagttgtcc aatgctggtg
     9481 acgcgatagc ttttacagcc atatccttcg gcaatccggg caaaatccac aggaatgagt
     9541 cccccctcct gacgaccgtt atcctgttta tggaaacgaa actcggtaaa gaaactgtcc
     9601 ataccgtgtt ccatttgaag attatttata caaccattag tcatgttatc cagcaaaata
     9661 acattaattt tcttccccat ctggaccgaa gttaccagct cagaatgcag catcataaat
     9721 gagccgtcgc caacgaagct atatacctcc cgctccggtt gcgccaactt cgcccccaat
     9781 gcggcattga cctcatagcc catacaggaa tagccatatt ctacgtggta atcattatcg
     9841 ccacggcttt gccagatgcg ctgcagatcg cctggcagac ttcccgccgc agcaaccacc
     9901 agcgcgtttt ctggcagcat tcgatttagt actccaagaa cgcgcgtctg tgtcagccat
     9961 gaatgagtaa tttcattgaa ctcttcaaac actttttgag tatccatatg atccacaatt
    10021 tccggtttga aatccttacc gctgtattcc gctgcataga cgcgatccac ttccgccaga
    10081 tattgagaac gaacacgatg gatcgttttc ccccattgtg atatgtaacc ttccggagcc
    10141 agtcgttcgc aaagctgcgt cagggcaaca cgggcatcag cctgcaattg cactccatcc
    10201 agtttgaaga cgtcaaaacg gctgacgttt atattcagat agcgcacatc aggattctgg
    10261 aaaatccatt tagaagaggt tgtgaaatcg gtataacgcg tacccacacc aataatcaga
    10321 tcggcctcct gagccagcaa attcgccgcc agcgatccgg tttcaccaat cccaccaaca
    10381 ttcagcggat gcgcagaact gagcgcacct ttaccagcct gcgtttcagc aatcggcaga
    10441 tgatattgtt cggcaaatcg cagcagttcc tgttcagact gcgaatactt cacaccccca
    10501 ccgcaaataa taattggtct ctggtggcga agaataagag caactgcatc attcagcatt
    10561 agctctgttg gcacgtggcg ctcaagacga tagacccgac gtgcaaaaaa atagtctgga
    10621 taatcccagg cttctccctg aacatcctgc ggcaaacaaa gcgtcacagc tcccgtatca
    10681 gccggatcgg taagcacccg catcgcgcta atgcaggcgc tcattagctg ttccggacgg
    10741 ataatgcgat cccaatagcg actcactgca cgaaaagcat cattggtgct cagcgtcagg
    10801 tcatgctcct gttcaatctg ctgcaacaca ggatcgggtt gacgcgatgc atacacatct
    10861 cctggtagca gaagcaacgg gatacggttc gccgtcgcag tcgccgccgc cgtgatcata
    10921 ttagcggcac caggccctac ggaggaggtg caggcataaa tctgctgacg acgcatctga
    10981 cgcgcaaatc ctgttgctat atgtgccata ccctgttcat tgcgtccttg atgaacgcta
    11041 agttgaccac actcttcttc cagagcctgc cctaatccca cgacattccc atggccaaaa
    11101 attgcgaaaa taccttttac aaacttgatt tcgcgaccgt ccacttcaat gtactgatta
    11161 tcaagaaagc gcaccaaagc ctgcgccata gtcaacctga ttgttttcat gcactccacc
    11221 tatcttatca accgtttgac tgtcgttacc tacgtacgca ctgctacagc cccgggcata
    11281 aaacactcac cacaagaaaa aaattccgca aacgcaatta tatgaaacat atttttcatt
    11341 tgtgagaaga agatcaaaaa acgattaaaa atttcgagaa tagctaaaat acgaacaaag
    11401 aagaatgaac attatgcttc caaaattaac tatacttatc aacaaattaa tagcttttta
    11461 gttgaagtaa gtctaatgac agagttgtaa catagaatct aaaagcatga ttatcgtcaa
    11521 aaaataatgg aattattttt tcctttttgt tgaaatcgaa attatataac cttagtgtaa
    11581 acaaaaatca cacggtcttc tgtaggagga tatctctatg aacaacacag tcagcagtga
    11641 gcaaaaaacg ctggacatta tctgtctggg gcgtgtcgcc gttgatttat atggtcaaca
    11701 aatcgggtca cgtctggaag acatgacaac ctttgcaaaa tatttaggag gctcatcagg
    11761 caacgtcgct tacggcacag caattcaggg actacgttcc gccatgctgg cacgtgtggg
    11821 cgatgaacat atgggacgtt tcatccgcga agaattaaac cgtgttggcg tcaatacaca
    11881 atgcctgttg actgatcctg aacgtctgac agccctggtg atccttggta ttaaagatca
    11941 ggacaccttc ccgctgatct tttatcgaga taactgtgcg gatatggcgc tggcgcctga
    12001 ggatatccac gaaacattca ttgcctcttc acgtgccctg gccgtcaccg gcactcatct
    12061 ttctcacatc aatacccgcg ccgcagtgct taaagcgctg acctatgctc aaaaacacgg
    12121 tctgcgtaga gtgctggata ttgattatcg tccggtacta tgggggttga catcgctggg
    12181 ggatggtgaa acacgtttca tcgcttcacc acaggtgact gaacagcttc aacaggtatt
    12241 gcaccatttt gatctgatag ttggcaccga agaggaattt catatcgccg gcggcagtac
    12301 tgatacgttg accgccctgc gccgtgtgcg tcagctaacc tctgcggtat tggtatgcaa
    12361 atgcggcgca ttagggtgtt cagtatttga aggaaacatc gccgacgact ggtctcaggt
    12421 gcagatgcac agcggtgtac gtgtggacat cctaaatgtg ctgggtgcag gagacgcctt
    12481 tatgtcggga ctgcttcgag gctatctgaa cgatgaaggt tgggagcaag cttgtcgtta
    12541 tgctaacgca tgtggcgcat tagttgtatc gcgccacggc tgcgcccccg cgatgccaac
    12601 caaacaggaa ctggatgact atctctcccg tgagcacgct atcaccagac ccgacaaaga
    12661 ccctcacctt aaccatcttc accgaacgac aacccgaaaa cagcactggc cggaactgtg
    12721 tgtattcgcc tttgaccatc gcaatcaact ggtggatatc gccagagcga caggtgccag
    12781 cgagttcact atccctgagc tgaagatgct actactggaa ggcgcacgtc aggcggcgca
    12841 cgaggctgga ttactgcata actgcggcat cctggcggat actacttttg gtcagaaagc
    12901 acttaacgac gtgactggtc agggatggtg gattggacgt cctatcgaaa tgccggggtc
    12961 atatcctctg aagctggagc acggtaatat cggttcacaa cttgtcagtt ggccgaaaga
    13021 gcacgtcgtg aaatgccttg tcttctatca cccactggat gcagaatccc tccgccttaa
    13081 gcaagaagca ctcatcgatg agatataccg ggcctgctgc cagtctggac acgatctgct
    13141 gctggaggtc atcttgccac gcgacgcagc agatcaaagc gagcattact atacacagat
    13201 tgtggaacgt ttttacctgc tggggatcca gcctgactgg tggaagcttc caccgttaag
    13261 tccggctcgt tggcaagaaa tcagccagca tattgagcgc tacgatcccg aatgccgcgg
    13321 tattttaata cttggactgg atgcaccgga aagcgagctg aaatctggct ttgccgccgc
    13381 tgcggatata ccatgggtta aaggcttcgc cgtaggtcgc accatctttg gcgagccatc
    13441 acggcagtgg ttcagcggcc agattaacga tgaacagctc atcgcagcag tgaaacaaaa
    13501 ataccgcaca ttgatcgact actggcgcga atatcgtccg gcgctttagt atccgtctgc
    13561 attacgtccc gcgtctgtat tcttgctcgg ggcgtattca aagatgtttc atgaccgtcc
    13621 tactttcctc atcatcccga cagtgctgtt tcgtatctgc attaatgata tttctgaact
    13681 gtctggctat tggtagttcc ctcgtcacgt tgccgcagaa cgtacaacag ctgtaccatc
    13741 tgagcaatct tctgatcggt acgctacagg cagcttgacg gttaatttcc attttactca
    13801 accgaggggt ggcagaacga aaaaacgaca cctcagacgg gcgacagacg gttatccgtg
    13861 gccagggtca ggtcgcctgt gctgaatcag ctccctcatc ttgctggtaa tcctgcttga
    13921 ttaacccgtt ccactactgt catggagttt atattaacgc atcccgtttt tcacagcttc
    13981 cattgttctg acattgacgg taggttgcag ctttatatcc gtacatggac tcccgatttt
    14041 gtgcaaaaaa gatgcttcag ttggggttca gactgccccc gtacattcgg cttcatttta
    14101 cagttttttg cagagccatg acggatatct gcgcacctat tccttatcgc ctttatgacc
    14161 tttgatggtc gtcggatata tcatattttg ctggtgtcgg tctgaccgat cttccgtctc
    14221 actttcctgc gcaattcatt tcagtaggcc acttcagaca aacttttctc tacaacatac
    14281 tttaa
//
LOCUS       DAZGQL010000120        14213 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.120, whole genome shotgun sequence.
ACCESSION   DAZGQL010000120 DAZGQL010000000
VERSION     DAZGQL010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14213
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.120"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            351..1241
                     /locus_tag="ACHKIR_004331"
     CDS             351..1241
                     /locus_tag="ACHKIR_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9991982.1"
                     /translation="MPPHKIRQEWLTLDEAAEVIRQHNITLLPADLIRHALHGDLSLS
                     VYFPSPVLLRPLNPPCRFACRFSKPPFPPSQCNKPGNRKVMANSPPCRRDPTLQLTTS
                     HLWNLPLIGIERLLLHQQLAKTLNRPCPNDTHCCNQIGIVVCDENDSYYQLCCRKSLR
                     ELLLELLATQNNLQEELHLLIGQLYGNGHNDLKSRLSLSVCYLQDTLPPDALFIIRRT
                     HLEAFLTRHQPESKERITSALSRLLWLACKHNPQLDDEMLEHPYKLLNIFECWAREEG
                     MHLPLSPETLKTALQRGAPP"
     gene            1380..1574
                     /locus_tag="ACHKIR_004332"
     CDS             1380..1574
                     /locus_tag="ACHKIR_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO9991983.1"
                     /translation="MNSDRFLRLRQVEDKIGFGKSWIYRQIQLQQFPPSIRLNSRHVA
                     WLESEVDAWIHQRIRLTRDV"
     gene            1591..2874
                     /locus_tag="ACHKIR_004333"
     CDS             1591..2874
                     /locus_tag="ACHKIR_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001728497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB family protein"
                     /protein_id="HFO9991984.1"
                     /translation="MCASHLLNRWLHGASNTGEQEMVQVSLEQIQPFSLNPRVTRNPG
                     YDVLKASILARGLDNPPVLTRRPGDEKYMLASGGNTRLAILNELWQETQDERYHRVCW
                     PFRPWPETLSPQQGEVQCLIGHLAESDLHNGLMFIERAEGVLHLRDLYLEAGIECPTQ
                     LALAEQLTRDGYPVSQSQISRMLQTVDWLLPCIPNALYGGLTRKVIDRLLSLRSAAEQ
                     VWVKLMPLERQPEFADLFSTALAIFNGEPEAVVPQLVQDELLGEMSCRSGMSWNTLLA
                     ELTDGQSKRQVLLGPPAEKVLWPPPPEVEKAPLPEPEPPTEPAAPDEPKPSPLVHTAQ
                     SLCACWGLDACIQPDDSAPAGFTLAPCHDFPHPAAQRCYQVLAALNGDTSTPMPALEL
                     LLSPDYNNAQIQQLMQLISLCRQRRLREVRHDTLS"
     gene            2858..3415
                     /locus_tag="ACHKIR_004334"
     CDS             2858..3415
                     /locus_tag="ACHKIR_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009654413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2857 domain-containing protein"
                     /protein_id="HFO9991985.1"
                     /translation="MTPSVNQAVLTHIVQALKEGQIRYCESLGFSPQELCELTRLTAD
                     DILFLSNSAVQFITTHIDHEMLGRMLARMEQERLFQQRLEEALSLGASIEFINHYFGL
                     STPEVCARRRLIGLFVRQGRNNAPDEQVETLVWNEWQKTGVKKLDSPEALTAMMAMAR
                     EHDLSLTVIWNLLRQWTQEKLLHEE"
     gene            3405..4277
                     /locus_tag="ACHKIR_004335"
     CDS             3405..4277
                     /locus_tag="ACHKIR_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012737303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STY4528 family pathogenicity island replication
                     protein"
                     /protein_id="HFO9991986.1"
                     /translation="MKSDDRLLFLGNPQQSVPKRLLLARNLSPRDKFAWQLLRLHARD
                     DGSGVFPSYNELQGWLSDQPEGEKASRSTVSNALMMLRITRWLSLCQRLRDEQSGRIT
                     GNLYALHDAPLTHADACRMDSEYLSLLTACSRHRNRRIRNIAIGLLLTLQQPVDGGRT
                     CTESPLKTAPGLNRGLSKKCPSLQLRLSNVAGAGGQPEPHCVHTGTDLNNKHQKRTPD
                     LHWPVDNPFSASERRAAERAMRNLDPALCQQVLDDCLWRIAAHDIHRPLAYLLATLEK
                     ARRGEFNQLRRRRR"
     gene            4437..5048
                     /locus_tag="ACHKIR_004336"
     CDS             4437..5048
                     /locus_tag="ACHKIR_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009654446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL_4669 family integrating conjugative element
                     protein"
                     /protein_id="HFO9991987.1"
                     /translation="MPGTTLFSDIRITLHTRVAARLWQAHPTGMLLCLALLRRLLRAE
                     EADDPWAAHWLKQLRIRLNLIAHLLKQKNRRLDHAFASLPGAIHTTQVSNPSPTEFIL
                     PLALFSPPGSRLLQQLIDYDLLVRRTLLAWHLGLITQAEKRDFIATIPRLMLQVFSFV
                     NRFRTTGVTRADVRANSPLAQSMAHKLGNLPKKMLTEIQRDVQ"
     gene            5038..5463
                     /locus_tag="ACHKIR_004337"
     CDS             5038..5463
                     /locus_tag="ACHKIR_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3158 family protein"
                     /protein_id="HFO9991988.1"
                     /translation="MFNDDDDERSARKSSLKGLLKAFKGKGELRALVQELQALRLQLQ
                     QNQPVLKQAVYELPGRNVPLILCSNPARSGVSYLRWRNLDNNRSGSVALQEAIQQPDA
                     SDELRDALMALEWRRHVLNTQLSVVMFMLRRIQPLTTLV"
     gene            5655..7460
                     /locus_tag="ACHKIR_004338"
                     /pseudo
     CDS             5655..7460
                     /locus_tag="ACHKIR_004338"
                     /inference="COORDINATES: protein motif:HMM:NF013311.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
     gene            7568..7750
                     /locus_tag="ACHKIR_004339"
     CDS             7568..7750
                     /locus_tag="ACHKIR_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011094427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9991989.1"
                     /translation="MAKNTGDGYRKGSVTDRTQVQNPQNGNYTKRDTTTGRFIDQKQD
                     GTPFKGVAKEVDDRRK"
     gene            8034..8477
                     /locus_tag="ACHKIR_004340"
     CDS             8034..8477
                     /locus_tag="ACHKIR_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STY4534 family ICE replication protein"
                     /protein_id="HFO9991990.1"
                     /translation="MNDTHYFNLYTSGLGYLNRIREVTPKKGEPFLCCDIAALRGEAS
                     NAEHTWFDCRVSGKEAQKLVRRCIDACEQKRKVLIQFRLGDLYADTFVYSKGDRKGET
                     GVSLKAHLLHIGLIKIDGEEVYRAEREAEPQPAPAALPAEPVAQF"
     gene            8551..8931
                     /locus_tag="ACHKIR_004341"
     CDS             8551..8931
                     /locus_tag="ACHKIR_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001728506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9991991.1"
                     /translation="MNTVLNSREEALDRGKLINLDDEALDAGLPLDLAITLPAHKHCL
                     EDRNDNAIRDLLKGVEMAAVVAFISHQPPQRTIRIDYRSVLTQKTNGSTQQQTVRLTL
                     RVHFSEQGIPIAATLGLMGERFPY"
     gene            9045..9701
                     /locus_tag="ACHKIR_004342"
     CDS             9045..9701
                     /locus_tag="ACHKIR_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000826209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilL"
                     /protein_id="HFO9991992.1"
                     /translation="MKRLLTALLLFIFNGVAASPATPEVVRYDRYLLVSTSPQQPPLE
                     QLTTLSVPPGFHPTVGEALQYLLRDSGWSLCLSDARRAQLYGLPLPLNQYHTGPLRLK
                     AALQLLAGPAWRMTADENRREVCFTSDHSLPQTVAYRHPVSLHPLGPLTAPFFLAGTV
                     QRGDIRLAVIVPPEALYLHQVVWLRIGEQWHGWRLARFDERRAVFRHGKRQLTLKVPE
                     "
     gene            9705..10379
                     /locus_tag="ACHKIR_004343"
     CDS             9705..10379
                     /locus_tag="ACHKIR_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03759 family integrating conjugative element
                     protein"
                     /protein_id="HFO9991993.1"
                     /translation="MKRLILLLLFTASTWAQPLQVQTSQNVGSALQPLTTQQQATVWG
                     ITPEEWQRYLTLKQQARGVWSPGLDPLTTLGVEADTDHERQRFAELLVRQEYQRLEKE
                     LAFQRAYDAAWQRLYPGLTPLKTTSASTSRVSLFVKDDCPPCDSLLRTLLAQQRPLDI
                     WLVGSEGDDNRVRRWAMAHGIDAKLVGEHSITLNHDAGRWLLLGQSKIPVALERKGEQ
                     WREVLP"
     gene            10358..10876
                     /locus_tag="ACHKIR_004344"
     CDS             10358..10876
                     /locus_tag="ACHKIR_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009654414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HFO9991994.1"
                     /translation="MARGPALILLLPCLALAQPLPEAYLRTAEHFRVPPELLWAVTQT
                     ESGTNLSGRHLPWPWTLNISGTGYRYASREQACQALLVAIRRTSPKRIDVGLGQINIG
                     WHQAYFDSPCDALYPLQNLSLTAQLLRQRYDERPGSWLNAAARYHRPAGGAPAARYRK
                     LVSRHLQQASPP"
     gene            10873..12891
                     /gene="traD"
                     /locus_tag="ACHKIR_004345"
     CDS             10873..12891
                     /gene="traD"
                     /locus_tag="ACHKIR_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001728513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HFO9991995.1"
                     /translation="MSRYALEALLRPPVEFYSTVIAGCGALICLTQAPLLMLTPWQAR
                     MMASALMLLALRRGWQGWQIVRYQRNLLRLPRYALTSRKIPVSRQRLFLGKGFRWQPI
                     HTQRLYDCLQPEAARWLRPGWCYRLARHIEKRSEHTLPGLARLLSADRWLNPVRPLPP
                     VGGNPWLHGVELKECDVTLPLSERPGHTLVLGTTRVGKTRLAELLATQDIRRGEVVIV
                     FDPKGDADLLKRMWTEAQRAGRGDRFILFHLAYPEFSARYNAIGRFGRISEVASRLTG
                     QLSGEGNSAAFREFAWRFVNIIARALVALGQRPDYQLISRHVVNIDALFIDYARIVLP
                     RHNTRSWEVVVQLAERVNDKNTPRHMLGRDPHVVALEQYLNAEKLYDPILDGLLSAVR
                     YDKTYFDKIVASLLPLLEKLTTGTLAALLAPDYHDLDDARPILDWQQAIRQKAVVYVG
                     LDALSDSVVAAAVGNSMFADLVSVAGHLYKFGLNDGLPGNSEKPPPINLHCDEFNELM
                     GEEFIPLVNKGGGAGIQVTAYTQTLSDIQARIGSAPKTGQVVGNFNNLIMLRVRETAT
                     AELLTKQLPQVEVRSRQVSSGVTDTPGGINGFTSNTCEQVTTRNVPLLDTAALIQLPK
                     GQAFALLEGGQLWKIRLPLPVADKQCPLPDSVAQLAAWMQARQQGLTE"
     gene            12888..13535
                     /locus_tag="ACHKIR_004346"
     CDS             12888..13535
                     /locus_tag="ACHKIR_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4400 domain-containing protein"
                     /protein_id="HFO9991996.1"
                     /translation="MSELRPPPSRPPQGPIGWLLSLAGRIIGLVIGTLVLRVVLELVG
                     LYFWWPQEGSRHVFQVMRQEKVELIIAVQPHPLGKEIRVLLEHGITRVNDVRHKAVSR
                     LFHEPVTVLAYTMISFALRLTWLVIMLPLLCLSAVTGLTEGLVHRDLRRFGSGLESIF
                     LHRYVIRCGSSVTTTLWVVYLAQPFSLPAMLVLLPAALWSGIAVWMAAGSFKRWL"
     gene            13601..13846
                     /locus_tag="ACHKIR_004347"
     CDS             13601..13846
                     /locus_tag="ACHKIR_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012822667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="HFO9991997.1"
                     /translation="MGVMSVRLNNNLSTQLEALSKATGRSKSWLANQAIEDYVAREAW
                     QVAEIEQAIQEADAGDFATSDEVDKLFQRLGVKPDGN"
     gene            13836..14120
                     /locus_tag="ACHKIR_004348"
     CDS             13836..14120
                     /locus_tag="ACHKIR_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010286547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO9991998.1"
                     /translation="MEIKWLRKAIANLEAEFRYIAEDDPQAAERFVNEVWRLTQLLKE
                     QPAMGREGRVPETRELILHNYPYIIPYRVRNNIIQVLRVFHTHRRLPVNW"
BASE COUNT         3106 a         4369 c         3921 g         2817 t
ORIGIN      
        1 aacagacacg taatatttac caatatccct gtctattttt aaaaggaata atgggggggt
       61 gttaggattt atagactgca actctttacc aattaagagt ctttcaatat catttataag
      121 ctcttcaaaa ttcataacag aagcctttat ccattgatat tatactaatg gatatcatac
      181 catatttatg cctattcgta atcattgatt tcctaaataa aacagtaaaa aacctaaaaa
      241 aaacaccaat agatcacata caaaacaacc agttaaaaga actcacctta cctcgcttta
      301 gttggctttt ttagatagta ctcatcatat gtaactaata ggagttaccc atgcccccac
      361 acaaaattcg ccaagagtgg ctgacgctgg atgaggctgc agaagtcatc cggcagcaca
      421 acatcacgtt gctgcccgcc gacctgatcc gccatgccct gcacggcgat ctgtcgctct
      481 cggtctactt cccttcgccc gttctgctga gaccactcaa tccgccttgc cgctttgcct
      541 gccgattcag taagcctcct tttccacctt cgcagtgcaa caaacccggc aaccgcaaag
      601 tgatggcaaa ctcgccgccg tgcagacgcg acccaactct gcaactgacc accagccatc
      661 tgtggaattt gccgttaatc ggcattgagc gcctgctgct ccaccagcaa ctggcgaaaa
      721 cgttgaatcg cccttgcccc aatgataccc attgctgcaa ccagattggc atcgtggtct
      781 gcgatgaaaa cgacagctac taccaactct gttgccgcaa atcgttgcgg gagcttttac
      841 tggaactgct cgccacgcaa aacaacctcc aggaagagct tcatctgctg attggccagc
      901 tatatggtaa tggccacaat gacctgaaat cccggctcag tctgtccgtc tgctacctgc
      961 aggacacgct gcctcctgac gcactgttca tcatccgccg cactcatctc gaagccttcc
     1021 tgacccgtca tcagccagaa tcaaaggagc ggatcacctc cgcgctttcg cgtttgctgt
     1081 ggctggcctg caagcacaac ccgcaactgg acgatgaaat gctggagcat ccctacaaac
     1141 tgctgaatat tttcgaatgc tgggcgcgtg aggaaggtat gcacctgccg ctgagccccg
     1201 agacgttgaa aaccgcactc cagcgcggcg caccgccgta acgcgatcaa ctaacccttt
     1261 caactaacaa ctctcactaa cccagataag gatttttact gcctggcagg gacgctgctg
     1321 ctttgataac cacgcctatc gtgctgatgt gaacgaatct ggacacagga ggaatggtta
     1381 tgaatagcga cagattttta cgcttacgcc aggtggaaga taaaatcggc ttcggtaaat
     1441 cgtggattta tcgacaaatc cagctgcaac agtttccgcc gtccattcga ctcaatagcc
     1501 gccacgtcgc ctggctggaa agcgaggtgg acgcgtggat ccaccagcgc atccggctta
     1561 cccgcgatgt ctgaatgccg gaggaagatt atgtgcgctt cacacctgct caaccgctgg
     1621 cttcacggcg ccagcaatac cggagaacag gaaatggtcc aggtcagcct ggagcaaatc
     1681 cagccgttct ccctcaatcc ccgcgtcacc cgcaaccccg gctatgacgt gctgaaggcg
     1741 tcaattcttg cccgtgggct ggataacccg ccggtactga cccgccgccc cggtgatgaa
     1801 aaatatatgc tcgccagcgg cggaaatacg cgcctcgcca tcctcaatga actgtggcag
     1861 gagacgcagg atgagcgtta ccaccgcgtc tgctggccgt tcagaccgtg gccggaaacg
     1921 ctgtcaccgc agcaaggcga agtccagtgc ctgatcggcc atctggccga aagcgacctg
     1981 cataacggat tgatgtttat cgaacgggcg gaaggggtcc tgcacctgcg cgatctttac
     2041 ctcgaggcag gcattgaatg cccgacgcag ctggcgctgg cggaacaact cacccgcgac
     2101 ggctatcccg tcagccagtc gcaaatcagc cggatgctac agaccgtaga ctggctgctg
     2161 ccctgcatcc caaacgcgct atatggcggt cttacccgca aggttatcga tcgcctgctg
     2221 tcactacgca gcgcagcaga acaggtctgg gtaaagctga tgccgctgga acgccagccc
     2281 gaatttgccg acctgttcag caccgccctc gccatcttca acggcgagcc ggaagccgtg
     2341 gtaccgcaac tggtgcagga cgaactgctc ggtgagatga gctgtcgcag cggcatgagc
     2401 tggaatacgt tgctggcgga actgactgac ggtcagagca agcggcaggt gctgctgggg
     2461 cctccggccg aaaaggtcct ctggccgcca ccgccggaag tggaaaaagc accactacca
     2521 gagcctgagc caccgactga acccgccgcg cccgatgagc caaagccctc accgctggtg
     2581 catacggcac agtcgctgtg tgcctgctgg ggactggacg cctgtatcca gccggacgac
     2641 agcgctccgg ccggtttcac cctcgcgcct tgccacgatt ttccccaccc ggcggcacag
     2701 cgctgctatc aggtgctggc ggccctgaat ggcgacacca gtacgccgat gcccgccctc
     2761 gaattgttgc tgtcgccgga ctacaacaac gctcagatcc agcaactgat gcagcttatt
     2821 agcctgtgcc gccagcggcg gctgcgggag gtgcgccatg acaccctcag ttaaccaggc
     2881 agtactcacc cacatcgtcc aggcgctgaa ggaggggcag atccgctact gcgaatcgct
     2941 cggcttcagc ccgcaggagc tgtgcgagct gacgcgcctt accgccgacg acatcctgtt
     3001 tctcagcaac agcgcggtgc agttcatcac cacccacatt gatcacgaga tgctgggccg
     3061 gatgctcgcg aggatggagc aggagcggct gttccagcag cgtctggagg aagcgctgtc
     3121 gctcggcgcg tccatcgagt tcattaacca ctattttggc ctttccacgc cggaggtctg
     3181 tgcccggcgg cggctgattg gcctgtttgt ccgccagggg cgcaacaatg ccccggatga
     3241 gcaggtggag acgctggtgt ggaacgagtg gcagaagacg ggcgtgaaaa agctcgactc
     3301 acccgaagcg ctaaccgcca tgatggcaat ggcccgcgaa cacgatctgt cgctgacggt
     3361 catctggaac ctgctgcgcc agtggactca ggagaagctg ctgcatgaag agtgacgatc
     3421 ggctgctgtt tctcggcaac ccgcagcaga gcgtgccgaa gcgcctgctg ctggcgcgca
     3481 acctctcccc gcgcgacaag ttcgcgtggc aactgctgcg cctgcatgcc cgcgacgacg
     3541 gcagcggcgt cttcccctcc tacaacgaac tgcagggctg gctctctgac cagcctgaag
     3601 gcgagaaggc ctcgcgcagt accgtcagca atgcactgat gatgctgcgg atcacccgct
     3661 ggctgagcct gtgccagcgg ctgcgcgacg agcaaagcgg caggatcacc ggcaacctct
     3721 acgccctgca tgatgcgcca ctgactcatg ccgatgcctg tcggatggac agtgaatacc
     3781 tgtcgctgct caccgcttgt tcccgccacc gcaaccgacg catccgaaac atcgctatcg
     3841 ggctgctgct caccctgcaa caacctgtgg acggtggacg aacctgcact gagtctccat
     3901 tgaagaccgc ccctggtctt aatcgaggac tcagtaaaaa atgcccgagt cttcaattaa
     3961 gactcagtaa tgtggcgggg gctggcgggc agccggagcc gcattgcgta catactggta
     4021 cagatttaaa caataaacat caaaagcgta cgccagacct gcactggcct gtggataatc
     4081 ccttcagcgc cagcgagcgc cgcgccgcag aacgcgcgat gcgcaatctg gatcccgctc
     4141 tctgccagca agtgctggac gactgcctgt ggcggatcgc cgcccacgat atccaccgcc
     4201 ccctcgccta tctgctggca acgctggaga aagccagacg gggcgagttc aatcagcttc
     4261 ggcgcaggcg cagatagccc tgattttatg cgcaccgcat aaagccggtg taccaactat
     4321 caatcaaacc tcattcatcg gcgaaattaa ctttctgcgg accagatggg tttcccgtcg
     4381 cctgactcat tgtgcggacc gacactgcgg gcatctgaat ccatggagaa tttccgatgc
     4441 ctggcacaac cctgttcagc gatatccgca tcaccctgca tacccgcgtc gccgcccggc
     4501 tgtggcaggc gcatcccact ggcatgctgc tctgtctggc gttactcagg cgtctgctgc
     4561 gcgccgaaga ggccgacgat ccgtgggccg cccactggct gaagcaactg cgcatccgcc
     4621 tgaacctgat cgcccaccta ctgaagcaaa aaaaccgtcg tctggaccat gctttcgcca
     4681 gcctgccagg cgctatccac accacgcagg tcagtaaccc gtccccgacc gaattcatcc
     4741 tgccgctggc gctattctca ccgcccggtt cgcgcctgct gcaacagttg attgattacg
     4801 atctgctggt ccgccgcacc ctgcttgcct ggcatctcgg ccttatcacc caggcggaga
     4861 agcgcgattt cattgccacc atcccacgcc tgatgctcca ggtgttcagc ttcgtgaacc
     4921 gctttcgcac cactggcgtg actcgcgccg acgtgcgggc gaacagtccg ctggcgcagt
     4981 cgatggcgca taagctcgga aatttgccca aaaagatgct tacggagata cagcgcgatg
     5041 ttcaatgacg atgatgatga acgctccgcc cgcaaaagct cgctaaaggg ccttttaaag
     5101 gcctttaagg gtaagggaga gctgcgcgcg ctggtacagg aattacaggc gctgcgcttg
     5161 cagctgcaac aaaatcagcc agtgctgaaa caggcggtgt atgaactgcc ggggcgtaat
     5221 gtaccgctga ttctgtgcag caacccggcg cgctccggcg tcagctatct gcgctggcgc
     5281 aatctggaca acaaccgttc ggggagcgtg gcgttgcaag aggccattca acagccggac
     5341 gcgagcgatg aactgcgcga cgcgctgatg gcgctcgaat ggcggcggca tgtgctgaat
     5401 acgcagcttt cagtggtgat gtttatgctg cggcggatac aaccgttaac caccctggtt
     5461 taaaattttg cgcaaagtta aaccctgagt gtcactttat cgattatttt aaacttaact
     5521 catgcacctt aaaacaggca ctgtgcgctt aatgcacgtt ttaaggtgca atttttacct
     5581 tctcctacca gtttcccctg tcgccttatc cctccggcac accatgctga actccctttc
     5641 accggagaca gcccatgcaa ctttacctgt gcgaaaaacc ctctcaggca cgcgacatcg
     5701 cccgaattct tgacgcccat aaccgcagcg acggctacct gcataacgcc accgtaacca
     5761 tcacctggtg tttcggccat ctgctgagca tggccgcgcc ggaggattac gatccggcgc
     5821 tgaaacactg gcggacggaa cagctgccgt ttattccccc ggcatggcgg ttggtgatcc
     5881 gcccggaggt gcataagcag ttccgtatca tcgaaaagct gctgaaacag gcggatgcag
     5941 tggtgattgc caccgacgcc gatcgcgaag gcgaaaccat cgcccgcgaa attctggaac
     6001 aatgccgctg gcgcggtccg gtccagcggc tgtggctgtc ggcgctggat gaggccagta
     6061 tccacaaggc gctcgccact ctgcgtcccg gcgacagcac ctttccgctg tatcaggccg
     6121 gactggcgcg cgcccgcacc gactggctgg tcggcatcaa catgacccgc gcctgcacgc
     6181 taatgaaccg gcggcacaag ggcgtgctct ccgtgggccg ggtgcagacg ccgaccctgc
     6241 gtctggtggt ggagcgcgac cgggagatcg cccgcttcac gccccgcccc tggtggcggg
     6301 tggatgcccg tttgcatgcg gagggttgtt cctttctggc gcagtggcag ccggatagtg
     6361 cgtactgtga cgacgaaagc cgctgcatcc gcgaatccgc cgcccgtgag gccacagcac
     6421 gcctgcaaca agccggaacc gcgctggtaa tggacgtgac aacccggcgg gagaaaacgc
     6481 cgccaccgca ggccttcact ctcagcgccc tgcagcaggc ctgctcaaaa cagtggggca
     6541 tgggcgcaca ggaggttctg gatatcgccc agctcctgta tgaaacccat aaagccacca
     6601 cctacccgcg caccgactgc ggctatctgc cgctctccat gcacgatgaa gcaccacaag
     6661 ttctggcggc gctggtcgcc agcgacgctt cactccagcc cctcgccacg cagcttaatc
     6721 tgcagcaacg ctcccggttg tgggacgaca gcaaaatcac cgcccaccat ggcatcatcc
     6781 cgacccgtaa aacggtagat ctgacgcaga tgaacgacag cgagcgcaag gtctacggcc
     6841 tggtacgcag ccattttctg gcgcagtttc tgccagacca cgaagtggat aaaacggacc
     6901 tgcgcctgaa ctgcgccggg gaaacgctga ctgcccgtgg ctcagtggtg attgtcagcg
     6961 gctggcgaca actgtttttg cgcgaaatgg caacgcactc accggaagaa acgcaggcac
     7021 ttcccgccct gcaacaggga caaacctgct aggtggaaca ggtggacgtt cgcgcccgcc
     7081 agacccagcc accggagcac tacaccgaag ggacgctgat tgccgcgatg aaaaatgccg
     7141 cccgttttgt cagcgatgaa cgcctgaaac agcggctgaa agaaaacgcg ggcatcggca
     7201 ccgaagccac ccgcgccggg attatccaga cgctccttaa gcgcggctat ctgattaagc
     7261 agcgtcgttt cctgctggca acggataccg ccagcacgct gattgacgcc ctgccggaaa
     7321 cgctgaaaga tccaggcacc actgcactgt gggagcaaat gctggatgac atcgcgacgg
     7381 ggaaaatggg actggaggcg ttcctggtgc agcaacagca gaacgtgaca gagctgatta
     7441 agtcgttacg gaatgagtaa ataacctgtt tcctgcatca cacttttact tttccctgat
     7501 tgctcgttca aattggcagt ctgatgatcc tctctgagga atattcctca tttttcggag
     7561 tgttaagatg gccaagaata ccggagacgg ttatcgcaag ggctctgtta cagaccgcac
     7621 acaggttcag aatccgcaga atggcaacta caccaaacgc gatacaacga caggtcgctt
     7681 tatcgaccag aagcaggacg gcactccctt taaaggtgtc gccaaagaag tcgatgaccg
     7741 ccggaaataa tatttagcta actttcaggg ccttagttat taaggccttt ttgcattctg
     7801 tacttccaac tttcacgccg taaaaacaat tccacctttc gtttctccct accgaagatt
     7861 caactaaaca ggtcaacccc aaaacccgcc atgcattcct gaaacgcgga ggcgcacagc
     7921 gcggaatgcc cgtactgcca gtaactcaga ggccttgcag ccaatgagcc aaacccgaag
     7981 cggggcgttc ccgcgcatgg ggcgctccgc accttcaaca cggagatacc accatgaacg
     8041 atacccacta cttcaacctg tacaccagcg gtctgggcta cctcaaccgg atccgggaag
     8101 tgaccccgaa aaaaggcgaa ccgttcctgt gttgcgacat tgccgcacta cggggcgaag
     8161 ccagcaacgc ggagcacacc tggttcgact gccgcgtcag cggcaaggaa gcgcagaaac
     8221 tggtgcgccg ctgtatcgac gcctgcgagc agaaacgcaa ggtgctgatc cagttccgcc
     8281 tcggcgatct gtacgccgac acctttgtct acagcaaagg cgaccgtaaa ggcgaaaccg
     8341 gcgtcagcct gaaggcccat ctgctgcaca tcgggctcat caaaattgac ggtgaagagg
     8401 tttaccgggc agagcgcgaa gctgaacctc agccagcgcc agccgcactg ccagcagagc
     8461 ctgtagcgca gttctgacgc acagcattca ccctgccggg gaaattcccc gtcggggcgt
     8521 ttccctggcc ttttaccgga gagacgcacg atgaataccg tattgaactc acgcgaagaa
     8581 gcccttgacc ggggcaaact gattaacctt gatgacgagg cgctggacgc tggcttgcca
     8641 ctggatctgg ccattaccct tccggcccac aagcactgtc ttgaagaccg gaatgacaac
     8701 gccatcaggg acttgctgaa aggtgttgaa atggcagcgg tagtggcctt tatctcccac
     8761 caaccgccgc agcgcaccat tcgtatcgat taccgctcgg tgctgacgca aaaaaccaac
     8821 ggcagcacgc agcagcaaac cgtcagattg acgttacgcg ttcatttttc tgagcaaggc
     8881 atccccatcg ccgccacgct ggggctgatg ggggaaagat tcccttactg atctgatttt
     8941 gcgcccggaa taacgggcgc tattttttga cgcagcaaac agttgtcggt agtccccagc
     9001 cctctcatcc ggcacgctcc tgtaaaagca ctgtggagga tgcgatgaaa cgactgctga
     9061 cagctctgct cctgtttatt ttcaacggcg tggccgcctc ccctgcaacg ccagaagtcg
     9121 tccgctacga ccgctatctg ctggtcagca cgtcaccgca acaaccgcca ctcgaacagc
     9181 ttaccaccct gtccgttccg cccggttttc acccgacagt cggtgaagca ttgcagtacc
     9241 tgctgcgcga cagcggctgg tctttgtgtc tctccgatgc gcgccgggcg cagctttatg
     9301 gcctcccgtt accgttgaac cagtaccaca ccggcccgct gcgcctgaag gccgccctgc
     9361 aactgctggc aggcccggca tggcgaatga ccgcagacga aaaccgccgt gaagtctgtt
     9421 ttaccagcga ccattccctc ccccagacgg tggcttaccg tcatcccgtg tccctccacc
     9481 ctttggggcc gttaacggcc ccgttttttc tcgccggaac cgtacagcgc ggcgatatcc
     9541 gactggcagt gatcgtgccg ccggaggccc tttatctcca tcaggtagtg tggctgcgta
     9601 tcggggagca gtggcacggc tggcggctgg cgcggtttga tgagcgccgg gcggtattcc
     9661 gccacggtaa acgccagctg acgctgaaag taccggagta agcgatgaaa cgcctgattc
     9721 tcctgttact gttcaccgcc agcacgtggg cgcaaccgct tcaggtgcag accagccaga
     9781 acgtgggttc agccctgcaa ccactgacca cgcagcagca ggccaccgtc tggggcatca
     9841 cgccagagga gtggcagcgc tatctcacgc tgaaacagca ggcgcgcggc gtctggtcgc
     9901 cggggctcga tccactgacc acactcggcg tagaagccga cactgaccac gaacgccagc
     9961 gctttgccga actgctggtc aggcaggagt accagcggct ggagaaagaa ctcgccttcc
    10021 agcgtgctta tgatgccgcc tggcagcgcc tgtatccggg cctgacgccg ctgaaaacca
    10081 cctctgccag tacttcccgc gtttcgctat ttgttaaaga tgactgcccg ccctgcgaca
    10141 gcctgctgcg taccctgctg gcgcaacagc gcccgctgga tatctggctg gttgggagtg
    10201 aaggcgatga taaccgggtg cgccgatggg cgatggcgca tggcatcgac gcaaaactgg
    10261 tgggtgaaca cagcatcacg cttaaccacg acgcgggccg ctggctcctg ttggggcaga
    10321 gcaaaatccc ggtggcgctg gaacgtaagg gggagcaatg gcgcgaggtc ctgccctgat
    10381 cctgctgcta ccctgtctgg cgctggcaca gccgctgcca gaagcgtacc tgcgcaccgc
    10441 ggagcatttc cgcgtgccgc cggagctgct gtgggccgtc acacaaaccg aaagcggcac
    10501 gaacctgagc ggcagacatc ttccgtggcc gtggacgctc aatatcagcg ggaccggcta
    10561 ccgctatgcc agccgggagc aggcctgcca ggcgctgctg gttgccatcc gccgcaccag
    10621 cccaaagcgc attgacgtgg gactcgggca aatcaacatc ggctggcacc aggcatattt
    10681 tgactctccc tgcgacgccc tgtatcccct tcaaaacctg tcgctcaccg cgcaactgct
    10741 gcgtcagcgc tacgatgaac gtcccggaag ctggctcaat gccgccgccc gctatcatcg
    10801 tcccgctggc ggcgcgcctg ccgcgcgata ccgcaagctg gtcagccgcc atctgcaaca
    10861 ggcctcaccg ccatgagccg ctacgcgctg gaggcgctgc tgcgcccacc cgtggagttt
    10921 tacagcaccg tcatcgctgg ctgtggcgca ttaatctgcc tgacgcaggc cccgctgctg
    10981 atgctgacgc cgtggcaggc ccgcatgatg gccagtgccc tgatgcttct ggccctgcgg
    11041 cgcggctggc agggatggca aatcgtacgc taccagcgta acctcctgcg cctgccgcgc
    11101 tacgcgctca ccagccggaa aatccccgtc agccgccaga ggctgtttct cggtaaaggc
    11161 tttcgctggc agcccatcca cacacagcgt ctgtacgact gcctgcaacc ggaagccgca
    11221 cgctggctgc gccccggatg gtgttaccgc ctggcaagac acatcgaaaa acgcagcgag
    11281 cataccctgc ccggccttgc ccgactgctc agcgccgatc gctggctgaa cccagtacgt
    11341 ccgctgccac ccgtaggcgg caatccgtgg ttgcatgggg tggaactaaa agagtgcgac
    11401 gtcactctgc cgctcagtga acgccccggc catacgctgg tgctcggcac cacccgcgtt
    11461 ggcaaaaccc ggctggcgga actgcttgcc actcaggaca tccgccgtgg cgaggtggtg
    11521 atcgtttttg atccgaaagg cgacgcggat ctgctgaagc gtatgtggac agaagcgcag
    11581 cgcgccggac gcggcgaccg ctttatcctg ttccatctgg cgtacccgga gttctccgcc
    11641 cgctacaacg ctatcgggcg atttggccgc atttcggagg tggcgtctcg cctcactggt
    11701 cagctttccg gcgaaggcaa ctcggcggcc tttcgcgagt tcgcctggcg ctttgtcaac
    11761 atcatcgccc gcgccctggt ggcgctcggc cagcgccccg actatcagtt aatttcccgc
    11821 cacgtcgtca atatcgacgc cctgtttatc gactatgccc gcatcgtgct gccgcgccac
    11881 aacacgcggt catgggaagt ggtggtgcaa ctggcggagc gcgttaacga caaaaacacg
    11941 ccgcgccaca tgctgggccg cgatcctcac gtggtggcgc tggagcagta cctcaacgcg
    12001 gaaaaattat acgatccgat cctcgatggc ctgctctcgg cggtgcgcta tgacaaaacc
    12061 tatttcgaca agattgtcgc ctcactgctg ccactgctgg aaaagctgac caccggcacg
    12121 cttgccgccc tgctggcccc ggattaccac gatctggacg acgcgcgtcc gatcctcgac
    12181 tggcaacagg cgatccgcca gaaggcggta gtctatgtcg ggttggacgc gctctccgat
    12241 tcggtggtcg ctgccgccgt cggcaattcg atgtttgccg atctggtgag cgtcgccggg
    12301 catctgtata aatttggcct caatgatgga ctgcccggca atagcgaaaa accgccgcct
    12361 atcaatctgc actgcgatga gtttaatgaa ctgatgggcg aggagtttat tcccctggtc
    12421 aacaagggcg gcggtgcggg cattcaggtc accgcctaca cgcagacgtt gagtgatatt
    12481 caggcccgta tcggcagcgc accaaagaca ggccaggtgg tgggtaactt caacaacctg
    12541 attatgctgc gggtacgcga aaccgccacc gccgaactgc tgacgaagca actgccacag
    12601 gtggaagtgc gcagccgcca ggtcagttca ggtgtcaccg acacgccggg cggcatcaac
    12661 ggctttacca gcaacacctg cgagcaggtg accacccgca atgtaccgct gctggatacc
    12721 gccgcgctga tccagctccc caaaggccag gcttttgccc tgctggaagg cgggcagttg
    12781 tggaaaattc gtctgccgct gcccgtagcg gataagcaat gcccactacc agattctgtc
    12841 gcgcaactgg cggcgtggat gcaggcgcgc cagcaggggc taacggaatg agtgagctgc
    12901 gaccgcctcc gtcacgccca ccacagggac cgattggctg gctattgtca ctggccggac
    12961 ggattattgg gctggttatc ggtacgctgg tgctgcgagt ggtgctggaa ctagtggggc
    13021 tatatttctg gtggccgcag gaagggagca ggcatgtttt tcaggtgatg aggcaggaga
    13081 aggtggaact gattattgcg gtacagccac atcctttggg gaaagagata cgagtactgc
    13141 tggagcatgg aataacccgc gttaatgatg tcagacataa ggccgtgagt cggttgttcc
    13201 acgaacccgt taccgtcctg gcttatacca tgatcagctt tgcgctgcgc ctgacgtggc
    13261 tggttatcat gttgccactg ctgtgcctca gtgctgtaac cgggctgaca gaaggcctgg
    13321 tgcaccggga tttaagacgg tttggctccg ggctggaatc catttttctc caccgttatg
    13381 tcatcaggtg cggcagttca gtcaccacca cgctttgggt ggtttatctg gcgcagccat
    13441 tttctctgcc cgcgatgctg gttttacttc ctgccgcctt atggtccggg atagcggtat
    13501 ggatggcagc aggaagcttt aaacgttggt tataagttgc tcaaaaaatc gcacctcact
    13561 acaatgtata caatgtgtac atgtgtatga ggccaccaat atgggcgtaa tgtcagtccg
    13621 tttaaacaac aatctttcca cgcaactgga agcattatca aaagcgaccg gacgctcaaa
    13681 gtcctggctg gcaaatcagg caattgaaga ttatgttgcc cgggaggcct ggcaagttgc
    13741 agagattgag caggccattc aggaagctga tgccggtgat tttgccactt cagatgaggt
    13801 ggataagctg tttcaacgtc tgggagtaaa gccagatgga aattaagtgg ctacgtaaag
    13861 ccattgccaa tctcgaagct gagttccggt atattgctga agacgatccg caggctgcgg
    13921 aacggtttgt gaacgaagtc tggcgactta cgcaactgct caaagagcaa cccgctatgg
    13981 gccgggaagg aagggtacct gaaacacgtg agctcattct tcataattac ccttatatca
    14041 ttccctatcg agtacgaaat aatataattc aggttttacg cgtgtttcat acccatcggc
    14101 gcttacctgt aaattggtga taaaaaacac aagacaagaa attttttaga gatggaattc
    14161 aaaccatgga caaacctatt tttaatatca taaacaaaaa attcattatt tca
//
LOCUS       DAZGQL010000121        13918 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.121, whole genome shotgun sequence.
ACCESSION   DAZGQL010000121 DAZGQL010000000
VERSION     DAZGQL010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13918
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.121"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..281)
                     /locus_tag="ACHKIR_004349"
     CDS             complement(<1..281)
                     /locus_tag="ACHKIR_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFO9991999.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVT"
     gene            complement(281..559)
                     /locus_tag="ACHKIR_004350"
     CDS             complement(281..559)
                     /locus_tag="ACHKIR_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO9992000.1"
                     /translation="MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAASCLA
                     DIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVNGDAYILDYEDYH"
     gene            complement(745..1755)
                     /gene="adhP"
                     /locus_tag="ACHKIR_004351"
     CDS             complement(745..1755)
                     /gene="adhP"
                     /locus_tag="ACHKIR_004351"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HFO9992001.1"
                     /translation="MKAAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAASSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMGADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RR"
     gene            complement(1889..3586)
                     /gene="maeA"
                     /locus_tag="ACHKIR_004352"
     CDS             complement(1889..3586)
                     /gene="maeA"
                     /locus_tag="ACHKIR_004352"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFO9992002.1"
                     /translation="MEPKTKRQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWHAEYRDYRRTSI"
     gene            complement(3743..3880)
                     /gene="sra"
                     /locus_tag="ACHKIR_004353"
     CDS             complement(3743..3880)
                     /gene="sra"
                     /locus_tag="ACHKIR_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HFO9992003.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            complement(3982..4197)
                     /gene="bdm"
                     /locus_tag="ACHKIR_004354"
     CDS             complement(3982..4197)
                     /gene="bdm"
                     /locus_tag="ACHKIR_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HFO9992004.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEHGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            4542..4973
                     /gene="osmC"
                     /locus_tag="ACHKIR_004355"
     CDS             4542..4973
                     /gene="osmC"
                     /locus_tag="ACHKIR_004355"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HFO9992005.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            complement(5029..5955)
                     /locus_tag="ACHKIR_004356"
     CDS             complement(5029..5955)
                     /locus_tag="ACHKIR_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFO9992006.1"
                     /translation="MSDTLLTLRDVHINFPARKNWLGKTTEHVHAINGIDLQIRRGET
                     LGIVGESGCGKSTLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLPVWRI
                     ITEPLWIAKRSSEQQRRALAEELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQ
                     PDVIVLDEPTSALDISVQAQILNLLVTLQENRELTYVLISHNVSVIRHMSDRVAVMYL
                     GQIVELGDAQQVLTAPAHPYTRLLLDSLPAIDKPLEEEWALRKTDLPGNRTLPQGCFF
                     RERCPLTTHGCEVRQSLTIREDEREIRCWRAL"
     gene            complement(5948..6934)
                     /locus_tag="ACHKIR_004357"
     CDS             complement(5948..6934)
                     /locus_tag="ACHKIR_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO9992007.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLKINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGHISLLGEDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGVTADVIHHPRHPYTIGLLQCAPEHGIPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPVLTAYGDNNHRCACWYPQQEVISV"
     gene            complement(6931..7827)
                     /gene="ddpC"
                     /locus_tag="ACHKIR_004358"
     CDS             complement(6931..7827)
                     /gene="ddpC"
                     /locus_tag="ACHKIR_004358"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HFO9992008.1"
                     /translation="MMLSEETSAVRPPKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMMGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQTLVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            complement(7824..8846)
                     /locus_tag="ACHKIR_004359"
     CDS             complement(7824..8846)
                     /locus_tag="ACHKIR_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO9992009.1"
                     /translation="MTFWSILRQRCWGLVLVVAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVDNIRQQLGLDQPLYVQFYRYVSDLFQGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFCALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            complement(8848..10398)
                     /locus_tag="ACHKIR_004360"
     CDS             complement(8848..10398)
                     /locus_tag="ACHKIR_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO9992010.1"
                     /translation="MKRSILFRPTLLAIVLATTMPVAHAAVPKDMLVIGKAADPQTLD
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGDLASSWKASDDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDAPDEHTVKFTLSHPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSWQKGQQLVLVPNPHYP
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTSKPTSLTFLYSDNDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            complement(10412..10993)
                     /gene="ddpX"
                     /locus_tag="ACHKIR_004361"
     CDS             complement(10412..10993)
                     /gene="ddpX"
                     /locus_tag="ACHKIR_004361"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HFO9992011.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIP
                     SEWWHFELPLAASYPLLADQFTCFISPGTQHAS"
     gene            complement(11251..13650)
                     /gene="dosP"
                     /locus_tag="ACHKIR_004362"
                     /gene_synonym="pdeO"
     CDS             complement(11251..13650)
                     /gene="dosP"
                     /locus_tag="ACHKIR_004362"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase
                     DosP"
                     /protein_id="HFO9992012.1"
                     /translation="MKLTDADNAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPQYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCINEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRI
                     EGTQFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNRDYL
                     LSTAHNAMDFIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWHDPLHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMQAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGIGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSMLPLK
                     I"
     gene            complement(13675..>13918)
                     /locus_tag="ACHKIR_004363"
     CDS             complement(13675..>13918)
                     /locus_tag="ACHKIR_004363"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFO9992013.1"
                     /translation="GAVSRSRTLCLVDFGLRFNHKGRHYFKAANGEDIALSLSIGAAM
                     FNGHPDYERLIQIADEALYIAKRRGRTRVELCKASL"
BASE COUNT         3362 a         3636 c         3490 g         3430 t
ORIGIN      
        1 tgcgtaacca gacggcgcag tcgcgacaca tccaccgttt tttcagcctc tgccaggctc
       61 caggcgtttt gcagattcag ccacatttgc ggcgaactgc cgatcaccac ggaaagttta
      121 atcgccattt ccggagtgag cgccgctttt ccggtcagca atcggcttgc cgttgagggc
      181 gcaatttcca ttgctctggc aaactcgcgc aggctgacat taagttcgtc cagtgattcc
      241 tgaataatgt cccccgggcg gggatgattg gccattttca tcagtgatag tcctcgtaat
      301 ccagtatgta tgcatcgcca ttgacgaatt caaatgtgat tcgccagttg cccgagacag
      361 atatcgccca aattccatcg cgatcgccgc ttaacgggtg tagtcggtaa ccgggcatat
      421 cgatatcagc aagacagctt gctgcatcaa tcacagcgag tcggtggcgt aatcgtttga
      481 cttgcgtcgg tataacgccg gaggttttgc cgagaagaaa caagtcacgc aatcccttgt
      541 gtctgaaatt cattatcatc tcttcatcct tgttccttca aaaggaacac tatcactatt
      601 gttccgtgca gcgcaacaaa tttcgctacg tttttgcgaa ttgtgagggg aaatatgggg
      661 gaggaaaaga agaagagtga aattgcggaa agcgtctcgc aaaacaggct gcgacccgaa
      721 catggcagtc gcagcaaagg cctcttagcg gcggaaatca atcaccatac ggccacggat
      781 tttgccttct tccatctcgg taaagatggt gttgatgtcc gctaacggac gcagggcgac
      841 tttcggcacc actttgcctt cggcggcaaa ctggaaggct tcggttagat cctggcgcgt
      901 gccgaccagc gaaccgacca cttcaatacc atccagcaca aggcgtggga tgtccaggct
      961 catagactcc ggcggcagac cgacagccac aacacgaccg cctgctcgta cagcatcaac
     1021 cgccgagtta aacgcagctt tagctaccgc tgtcaccacc gcggcgtgag cgccaccggt
     1081 tttttcttgc acaattttgg cggcgtcttc agtgcgtgag ttaatcgcta aatcagcgcc
     1141 catttcggtt gccagtttta actgctcatc attgacatca atggcgatca ctttggcgtt
     1201 aaagacattc ttcgcgtatt gcagggcgag gttacccaga ccgccaagac cgtagatagc
     1261 aatccactga ccgggacgaa tttttgacag tttcaccgct ttataggtgg tgacccccgc
     1321 acaggtaatg ctgctggccg ccgccgagtc caggccatct ggcactttta ccgcgtaatc
     1381 ggcgaccacg atgcactctt ccgccatccc gccatcaacg ctgtatccgg catttttaac
     1441 tgaacggcag agcgtttcgt taccactgtt acagtattcg caatgaccgc atccttcgta
     1501 aaaccacgcc acgctggcac gatcgcctgg ttttaatgag gtgacacctg ggccaacttc
     1561 tgccaccaca ccgatgcctt catggcccag aattacgccg gttttgtcac caaaatcgcc
     1621 attcttaaca tgaagatcgg tatgacatac accacaacac tccattttca gcagggcttc
     1681 gccatgtttc agtgagcgca gtgttttatc cgtaacgtca acatgatgat ccttcgtaac
     1741 aactgcagcc ttcatagttc ctccttttcg gatgatgttc tgcatagcag gtgaggcaaa
     1801 tgagatttat tcgccactac ccagtatgga tgagatctga aaaagggaga ggaaaattgc
     1861 ccggcagctt tcaccaccgg gcgcaggctt agatggaggt acggcggtag tcgcggtatt
     1921 cggcgtgcca gaaattatca tcaatggcct gttgcagagc ttcggcagac gttttcaccg
     1981 ccacgccttg ctgctgcgcc attttgccaa ccgcaaaggc aattgcgcgg gagactttct
     2041 gaatatcttt cagttccggt agtaccagac cttcgccgtt caggaccagc ggcgaatact
     2101 gagcaagcgt ttcacttgcc gacatcagca tctcatcggt gatacgtgac gcgccggaag
     2161 caataacgcc cagcccgatg cccgggaaaa taaaggcgtt gttacactgg gcgatagggt
     2221 agattttatc tttccatacc actgggttaa acggactgcc agtggcgacc agcgcgttac
     2281 cttcagtcca ggcgataatg tcctgcggtg tggcttccac gcgtgaagtc gggttagaca
     2341 gcggcatcac gatcggacgc ggacagtgtt tatgcatctc acggataatc tcttccgtaa
     2401 acagcccggt ctgtcctgag acgccaatca gaatatctgg ttttacatta cgcaccacat
     2461 ccagcagtga cagcacatcg ctgtcggtat cccagtcact gaggttttcg cgcttctgca
     2521 ccagtttggt ctggaaaggc agcaggttcg gcatcttgtc ggtcagcagg ccaaagcgat
     2581 caaccataaa gactttctgc cgcgccgctt cctcgcttaa tccttcacgc tgagtctggg
     2641 cgatgatcat ttcggcaatg ccgcaccccg cagagcctgc gccgaggaag acaatctttt
     2701 tctcgctcag ttgaccacct gccgcgcggc tggctgcgat cagtgtgccg accgttaccg
     2761 ccgcggtgcc ctgaatgtca tcgttaaaag aacaaatttc attgcgatag cggttaagta
     2821 acggcatcgc atttttttgt gcgaagtctt caaactgcaa cagtacgtcc ggccagcgtt
     2881 gtttcacagc ctggataaat tcatcaacga attcatagta ttcgtcgtca gtgatacgcg
     2941 gattacgcca gcccatatac agcggatcgt taagcagctg ttggttgttc gtcccgacat
     3001 ccagcaccac cggaagggta tacgccgggc tgatgccgcc acaggcggta tagagcgaca
     3061 gtttaccgat cggaatgccc atcccgccga tgccctggtc accaagcccc agaatacgtt
     3121 caccgtcagt caccacaatc actttaatat tatggttcgg cacgttttgc agaatatcgt
     3181 ccatattgtg gcggttctgg taagagataa acacgccgcg tgaacggcgg tagatctcag
     3241 aaaaacgctc acaggctgcg ccgacggttg gggtataaat aacaggcatc atctcatcaa
     3301 gatgattgtt taccagacgg tagaagaggg tttcgttagt gtcctggatg ttacgcaggt
     3361 agatgtgttt gtcgatttcg gttttgaatc cctgatactg gatccatgct cgttccgctt
     3421 gttcttcgat ggtttcgacc acttccggca gtaaccccag caggttgaag ttacggcgtt
     3481 cttccatgct gaaggcgctg cctttattca acaacggaaa ttccagcagt acagggccag
     3541 cgtaaggaat ataaagcgaa cgctgtcttt ttgtttttgg ttccatgtca ctcactcttt
     3601 tttgaatatc catccctggc acgcttttat cgtctttgct ttaccgccag ggcgtcggcc
     3661 ccaagtataa agcagataaa aacaaaaaca ccattgcgca ggcaatggtg tttaatcgtc
     3721 attgaggact gatggttatg aattactttt cagcggggcg ttttctgcct gttgggttat
     3781 ttaccacgct ggatttgtca ccttcggtaa ctattttgcg ctggttagaa attttatggt
     3841 ccagtccaag aatatgacgt gcctgacggt tcgatttcat tttaactcct caatcctgtt
     3901 actagtttta aggacaacat cgccgtagcg aagaaacacg tgctaaaccc ctaaattagg
     3961 ttgccgatca agcatagcac cttaaagcgt agggtgctga ccactgacca cataattgat
     4021 cgtttgctgg tagatatcac tgaggatgtc gttatcagat gcttccaccc atttggtcag
     4081 ctccatgaga atgtcatctt cagtgacaac accgtgctct gcccgaaggc cttgctcaat
     4141 ggcattaacc agagcgggtt ctgctgttga attttctgcc tgataataag taaacatagt
     4201 gattctccgt gtctgtgtat ttatggtgtc tgctacggat cgcagattta taaagcacat
     4261 tcagcatggc aaatatttgc cgcttcgttg ttaagattag tcctggttga tgatttttat
     4321 attttaactc catgatattg ataggtatta ttattgtgat gagttgattg atattgatgc
     4381 aatatctctt gagttatata tttccggtta tctattgtgg aaatttaatt taagtgcagg
     4441 aattatattt tcgccggatt ttattcggat tatcctgctt atcctcgtgc tgtttctcac
     4501 gtagtctata atttcctttt taagcccaca ggagagcaac aatgacaatc cataagaaag
     4561 gtcaggcaca ctgggaaggc gatatcaaac gcgggaaggg aacagtatct actgagagcg
     4621 gcgtgctgaa ccaacagccg tatggattta acacgcgttt tgaaggcgaa aaaggaacca
     4681 accctgaaga actgattggc gcagcgcatg ccgcatgttt ctcaatggcg ctttcattaa
     4741 tgctggggga agcgggattc acgccaacat cgattgatac caccgcagat gtgtcgctgg
     4801 ataaagtgga tgccggtttt gcgattacta aaatcgcact gaaaagcgaa gttgcggtgc
     4861 cgggtattga tgcctccacc tttgacggca ttatccagaa agcaaaagca ggatgcccgg
     4921 tttctcaggt actgaaagcg gaaattacgc tggattacca gttgaaatcg taaagcgttg
     4981 ccggatgacg cgtcaggcgt gtgattgcct gatgcgttgt tagcgtctct acagcgcccg
     5041 ccagcaccgg atctcacgtt cgtcctccct tattgttaat gattgccgga cttcacatcc
     5101 gtgggttgtc agagggcagc gttcgcggaa aaaacagcct tgtggcaacg tgcggtttcc
     5161 tggtagatcc gttttacgta atgcccattc ttcctccagc ggtttatcaa tggcggggag
     5221 ggaatccagc aataatcggg tgtatggatg tgccggagct gtcagcacct gttgcgcgtc
     5281 tcctagttcc acaatctgtc cgagatacat caccgccacc cgatcgctca tatgacgtat
     5341 caccgagaca ttgtgtgaaa tcagcacata agtcagctcg cgattttcct gtagcgttac
     5401 cagtaaattg aggatctgcg cctgcacgga gatatccagc gccgaggttg gttcatcaag
     5461 cacaatcacg tcaggctgcg aagagagtgc tctggcaatg gcgatacgtt gccgctgccc
     5521 gccggaaaac gcatgaggca agcggtcgag atactccgga cgaataccca cctgcacagc
     5581 cagctcctct gccaacgctc gccgctgttg ttcactacta cgcttagcaa tccagagcgg
     5641 ttccgtgatg atgcgccaca ccggtaagcg cggattaagc gaagagagcg ggtcctgaaa
     5701 caccatctgc ataatgcgtt gtgatcctga acggatgtac tgcccgtggc tcggttgcag
     5761 catacccatt aaaagctgtg cgagggtgct tttgccgcag cctgactcac cgacgatccc
     5821 taaggtttca ccacgacgga tctgtaaatc aataccattg atggcatgaa catgttccgt
     5881 agttttacca agccagtttt tacgggccgg gaaattgata tggacgtcgc gtaacgttaa
     5941 taatgtatca gacactaatg acctcctgct gcggatacca acaggcgcag cggtggttgt
     6001 tgtcaccata cgctgttagc accgggacgt tttcacactg tgcacctgcc gcatagcaac
     6061 gatcgcgaaa agcgcagccg tcaggcaaat gggtgaggtt tggtaccgtc cccggaatag
     6121 cgggtaatgg ctggcgcggt attccatgtt ccggtgcgca ttgcagcaaa ccaatggtat
     6181 acggatgccg gggatgatgg ataacgtctg ccgtcacgcc gctctcaatc acgcttccgg
     6241 catacatcac gtaaacgcta tcgcacagtt gcgacaccac ggccatatca tggctgatga
     6301 acagtaccgc agttccactg gcgcgggctt tatgtttaag cagacgcagt acctgcaact
     6361 gtaccgtgac gtccagcgcc gtagtcggtt cgtcggcaat aatcaattgc ggctcgcagg
     6421 agaatgccag cgcaatcatt acccgctggc gcataccacc tgaaagctca aacggatagc
     6481 gcgacataac ttccacggca tccgggattt gcatctcttc cagcagggca atcgctttag
     6541 ctctggcttc ccgacgactt attggttgat gatggcggat cacgtccatc atctgaagac
     6601 ctattcgacg tgtcggatta agggcggtca tcggttcctg aaagatcatc gccactcgtg
     6661 cgccgcgcca ctgacgaagc tgcttttccc gagcgttaag aacatcttct cccagcagtg
     6721 aaatatgtcc ccgatgtacg caataactgc ccgtcggtag cagacgcata atcaacattg
     6781 cagtgactga tttacccgag ccggattctc ccaccagacc gacaatttca ccgcggttga
     6841 ttttcaatga cacattgttg agcgcgtgaa cgtcgccgtt aaaaccgggg aaactcaaat
     6901 gcagttgttg aatgtccaga acgggttggg tcatgactgc tttcctcctg ctttcgggtc
     6961 aagcagatcg cgaataccat cgccaaagag attaaacccg acggcggtaa gcaaaatcgc
     7021 tgcccccgga aatgcgcaat accaccattg atcgagcaca tagttgcgac cattcgccac
     7081 catcgccccc cattcagcac tcggttgttg agcaccaagg ccaataaatc ccaacgtggc
     7141 ggccattaaa atcgcgctac cgatatccag cgatgcctgc acaatcagcg gcggcaggga
     7201 gttacgtaaa atatgccagt tgatcagatg ccagcgtgac gcaccaaagg ttttcgccgc
     7261 ctgaacatag gtatactggc gaacaactaa tgtttgcccc cgcgccaggc gcacataaaa
     7321 gggaattcgc acaatagcaa tcgccagcat ggcgttaaac aaactcggcc cgagagcggc
     7381 tgccagtgcc attgtcagca ccagcgaagg aatcgacagc ataatgtcca tgatgcgcat
     7441 gataatcgcg tctgcgcgtc cgccaagcac accggataga cacccgagta gcgaccccat
     7501 catacctgca atggcgacca ccactaatcc ggcgagaatt gattgctgac tgccgaccag
     7561 tacgcggctg aacagatcgc gtcccacttc atcggtgcca aaccagtgcg ctgcagaagg
     7621 cggcaaaagg cgggcggtta aatcaatggc gttgggatca tgcgtcgcca gccacggtga
     7681 aaaaatcatc atcaatagca ttaatacaat gatgactgcg ccggtcacgg taagcgggct
     7741 gcctttcagc atccatacca gttttgcacc gttaaatcgc gtttgttttg gtgggcgcac
     7801 ggctgacgtt tcctcgctta gcatcattca ccacctccac gtccgatacg cggatcaatc
     7861 cacaaataga gcaaatccac caccaggttc accagcacat aagcaaacga aaccacgacg
     7921 gcaaagccca tcactgccgg gaagtcgagc gcctgtattg atgttactac ccatgcaccc
     7981 attccgggcc aggcaaaaac ggtttcggtg agcactgcgc catacaacaa atcgcccagc
     8041 gccagtccca atacggtaat cgaggggatc aacgcattgg gtagcgcata acataaaacg
     8101 atataccagc cgggcaagcc gctggcccgg gcggtacgaa tgtaatcttc actcaattgt
     8161 tccagcatag ctgagcggat ctggcgagcg acaattccca ggtgaacgaa cgccagcgtt
     8221 aatgccggta agatgagatg ttgcagcgca ttgaagaaga cttcaccgtt gccttcaagc
     8281 agcgcatcga gcagataaaa gccggtaacg tgcgttggtg gatccagcca gtcatcaagc
     8341 cttccgccgc cgggaagaat ttgcagatga ccataaaaca gcacaatgac gcccagccca
     8401 agccagaacg caggtgtgga gattccggta atggccatta ttcgcaccag atgatccagc
     8461 cagcgatttc gccagactgc agagagtatg cccagcggga tgccaatcag aagtgccagc
     8521 agcagggcac aaaaagccag ttccagcgtc gccgggaaaa atatgcgcaa ctcttccagc
     8581 accggacgcc cggtacgaat ggatgttccc aggtcaccct gaaacaggtc gctgacgtag
     8641 cggtaaaact gtacgtacag tggctggtcc agtcccagtt gctggcgaat attatccacg
     8701 atagcatcgc tggcgcggtc acccgccagt aaccgcgccg gatcgccggg gatcaggtgc
     8761 gagataataa acgttatcac acagacgccc gccaccacga gcaccagccc ccagcagcgt
     8821 tggcgtaaaa tactccagaa ggtcacgtta tttactcatg gtattgatat tgaagacctg
     8881 ttccagcatg ggattgaaca caaagccttt cacctctttg ttcatcgcca gttggtagtt
     8941 tttctggaac aggtacacat aagcagcgtc atcaatgacg attttctgtg cctgctggta
     9001 gtcccgggta cgctgcgtct ggtcggtggt cgctagcgca ttgcgcagta gcttatcgac
     9061 ctcactgttt tcatagaacg agcggttacc cggcagacct tttttgtctg actcaaacca
     9121 gtaattcata aacatatacg ggtcggcaaa atccggactc cagttgccaa tcgcgatgtc
     9181 gtaatcacct ttacccactc tgtcgcgcat ggtggcgttc gccagctttt ccagcttcac
     9241 attgatgccc agtttgttga gactggattg tgtcgccaga gcgatcggtt cccagttcgg
     9301 atcattatca gaatagagaa acgtcaggct ggtgggtttg ctcgtcactt tatcccattc
     9361 ggctttggct ttcgtttcgt catggttgta ttgcattgcc gtcgcatcgt atccccacat
     9421 gccttccgga atcgggccgc gcatttgttt tccgttacca ctcagaatgc cgttaaccat
     9481 gccctgataa tcggtagacc aggaaatggc ccgacgcaga tccgcctgat taagtggcgc
     9541 tttgctgtta ttcagataca gataggtgac gcgcagtgac ggatactctg ccacattgac
     9601 tttattttcc tgcttcaggg cgttgagttg atccaccggc agcgcatcgg caatgtcaat
     9661 gtcgccacgg gagagctgca ggcgacggga ggcactttca ccgataattt ttaccgatac
     9721 tcgtttaaag ttcggtttat tgccggggta atgcggattt ggcaccagaa ctaattgctg
     9781 acctttttgc cagcttttca gcataaacgg tccggagcca gcggtatttt gcgcgaggaa
     9841 accacgggca tcatccgccg catgctcctt taagaccgcc ggattgataa tggatgcacc
     9901 gtcattcgcc agcgtgtaga ggaacggtgc gaatgggtgg ctaagggtaa acttcaccgt
     9961 atgttcgtcg ggagcatcaa tctttaaatc tttgggaaat gcttctgctg gcccctggcc
    10021 gatttttagt aaccgctcaa aagagagttt tacggcttcg gcagtgacag gtgtgccatc
    10081 ggcaaattta gcattgtcct tcagggtgaa cgtccactct ttttgatcgt cagacgcttt
    10141 ccagctacta gccagatcgc cttcaacgtc ggttgagcct ttatcaccgt ccgttttgta
    10201 ctgaaccagt cgctgataag acgggtaggt cactgtccag tcgttattat caattgtcac
    10261 cgctgggtcg agggtttgtg ggtcggcagc tttaccgatc accagcatat ctttcggtac
    10321 ggcggcgtgc gcaaccggca ttgttgtggc aaggacgatc gcgagcaatg taggacgaaa
    10381 caaaatcgat ctcttcatga cagtttcctg attagctggc gtgctgtgtg ccaggggata
    10441 taaaacaggt gaattgatca gcgagtagtg ggtaactcgc tgccaggggt aattcgaagt
    10501 gccaccattc gctcgggata ccgacaaagc caccgccagt cataatcgca ttcagcaaca
    10561 ggcgattgcg ctgagcggcg ggcgggacgg aagggtgata agcatgggaa cgttcgtgca
    10621 tttcatcgaa ccctgcaccc atatcgagga tgttcccgtg ctcatcacga agcgtcaggt
    10681 cgatcgccgt gccacggctg tgattagaac cgaccgtcac atcaacaaca tattgcgggt
    10741 ctgggcaggc ttgccacaac atcgcctgtg cttgttgtgg gcgatacgca tcgtaaatca
    10801 ccagttgtaa gcctgacagc tgggcgatac tgatactttt cgccagcgca gtaatcgcat
    10861 ccttgtgtaa cagacaacgc gcttgctgat aaatagcttt acctgtgatg ttatcagcgc
    10921 aggcgtattt caattcgatc tccagatcag ggaagatcac ggctaaatca accagttcgg
    10981 tggtatccga cataaacgat tcctgtataa gacaaaaatt gctgcgcttt ccgcttatgc
    11041 agatctcatg ccacgccggg ataagcgcca gaatgctggc ttaaagttat attttttaac
    11101 ctttgatcaa catttgtgca gcgtagtgca gttttggtgc aagagaggaa gttaaggaag
    11161 gaatctcccg gaatcgtagc tgaaatcaca gtatttaagt gacagtgtca cgttaaatga
    11221 aaacccgcgt gtgcgggcga gaggaatttg tcagattttc agcggtaaca tactgctcat
    11281 ccagcctgga atttcttcgg cgggcagggg gcgggaaaag aaatatccct gaataacgcg
    11341 acagtggatc ttgcgtagca tctcaaattg ctctttggtt tcgacgcctt ccgccacgac
    11401 ggttaaatta aggctttgcc caatgctggt aatggcttca agtaaggcaa ggatgcgttt
    11461 ttcggtcaga caacgatcga caaaactttt gtcaattttg atttccgtta ccggaagact
    11521 gactaagcgg gataatccgg aaaagcccgt gccaaaatca tctaccgata aaccgatgcc
    11581 catatcgcgc aggatctgaa tgcgtttaaa gatttcggta tcgtgttcca tcatcatgct
    11641 ttccgtgatt tccaccgtca gctggtggcc gtcgataccc caggcttgca ttgcatcaga
    11701 cacctgatta ggcagctgat tactgcgaaa gtgcagcgcc gacaagttaa cggataacgc
    11761 cgggatatga atattctggc tacgccattc tgctaactga cggcaagctt ccgcgatgac
    11821 ccagcgccca atattttcga tttcaccgat ctcttctgcg agaggaataa accgtgaagg
    11881 gggcacatga ccatgcaggg gatcgtgcca gcgagcaagg gcttcgatgc cgtacagttc
    11941 acccgtttct gcgaagattt gcggctggta aaccagtttc agttggttat tgctaattgc
    12001 ttctttcagc gccgcgccta aaaccaaacg ctcttttacc atttcgttca tcgccgggct
    12061 gaagaactgc cagccgttac cgccattttt gcgaataaaa tccattgcat tgtgagcagt
    12121 ggagagcagg taatcgcggt ttttacccac gtcgtagctg atgccaatgc tcaaggtgag
    12181 cgggaatggt ttatcgtcaa tcattatcgg cttgctgacc acattccgta gctcatcggc
    12241 gatctgcgta atgttactga cgtcgttttc caggctcacg aggacaaact gcgtaccttc
    12301 gatacgacag agatattgat ccggtttgag tttttcacga aagcgattga ccacttccag
    12361 caatgcctga tcggcccacg catagccaag gctatcaatc acatcctgaa tatggtcaac
    12421 accgatgaga tacaccaccg gagagacggc tttgtcgacc aggtcatcga ggtaattgtg
    12481 caggttattg cgatttggca gaccggtcat cggatcaaat tggatgagtt gttcaatatg
    12541 ctgacggctt ttttcctgtt ccagcgccag cgcggccata tgctggctga tatctgccac
    12601 gcgttcgata aaggcgctgg tttctgctcc tgacgaggtt ttaatttgca ggatccccgc
    12661 aggcgcacca tcacgctgac gaatggtcgc tgaccagctt tgcgcatttt gaacttctgc
    12721 accgtgcgaa gatgacgccc agtgtatcgg catcccgttg cgcagcgcga acagcgaaac
    12781 atgcgattcg ttgagtacag attcgatgtt acgacaaatg atttccccca tttcatgaaa
    12841 tggcgggctg ctgcacatgg cggcgagaat attgccttca agctggcgga tttgcctttc
    12901 ttcggtgata tccgagaatg tcattaccag gttctgcaga tgcgcgagca cgtcataaac
    12961 cgggctgata gaggctttaa tccagatttt ttcaccggtg cgcgtcaaca gcagaaattc
    13021 gtcctgatcg cgggcggttt tccatagcaa ctgctgtaaa cgaatacggt tatcagcagg
    13081 gaattctgga atgttcagga gtgtatcggg ctgcataccg ctggcttcgt taatgcagta
    13141 accaaacatt tcggtaaatg cgcgattgca ctgcacaata tggcgttccg gatcgaggac
    13201 aatcaccggt cggtcgagat ggtcaacggc aataatcaat tgtcgggtct gttctttttg
    13261 cgccatttct acgctggcat cccgtaccag cgccaggtaa taaactttcc cctcggcgct
    13321 cactttcgat agcgcaaaac gggtccagat tttactgccg tcttttttct ccagctgcag
    13381 ctcccgactc atcccctcga cgcgagcttt accaccttca cggttgtgac gaatgtattg
    13441 aggatgcgca ggacgcaaat cccgcggaat cagcatatca atgttattgc caatgacttc
    13501 ttcacgtttg tacccccaga gcttctctgc ggcggggttg aaaaacatca cttcatcatt
    13561 ttcgttaatt aacaccgcgc ccatcatatt ttgctcaagg gcggggaaaa aaatgccatc
    13621 ggcggcatta tccgcatcgg ttagcttcat gattacctct gcatcctggc gcatctaaag
    13681 actggcttta cagagttcaa cacgggttct acctcgtctt ttggcgatat acagagcttc
    13741 atcggctatt tgaatgaggc gctcatagtc aggatgacca ttaaacatgg cggcaccgat
    13801 ggaaagtgag agggcaatat cctcgccgtt tgcggcttta aaataatgtc gacctttatg
    13861 gttaaaccgc aggccaaaat caaccaaaca caacgtgcga gatcgactaa ctgcacca
//
LOCUS       DAZGQL010000122        13878 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.122, whole genome shotgun sequence.
ACCESSION   DAZGQL010000122 DAZGQL010000000
VERSION     DAZGQL010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13878
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.122"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(83..1591)
                     /gene="putP"
                     /locus_tag="ACHKIR_004364"
     CDS             complement(83..1591)
                     /gene="putP"
                     /locus_tag="ACHKIR_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HFO9992014.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKQASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQEG"
     gene            complement(1750..1959)
                     /locus_tag="ACHKIR_004365"
     CDS             complement(1750..1959)
                     /locus_tag="ACHKIR_004365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992015.1"
                     /translation="MMLQLCATVLNVTCVASLKMNEMLINEIDMTGIKKITQTFSLRQ
                     LTFLKGATAKNVRECNLMKNSVAEH"
     gene            2014..5976
                     /gene="putA"
                     /locus_tag="ACHKIR_004366"
     CDS             2014..5976
                     /gene="putA"
                     /locus_tag="ACHKIR_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HFO9992016.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLEKLCFEPE
                     LAGWNGIGFVIQAYQKRCPPVIDYLIDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGDGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQSVAAGEMSPVINPAEPKDIVGFVREATPR
                     EVEQALESAVNNAPIWFATPPAERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGRGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVIDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRQVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELQKEVFGPVLHVVRYN
                     RNQLPALIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            complement(6016..6654)
                     /gene="rutR"
                     /locus_tag="ACHKIR_004367"
     CDS             complement(6016..6654)
                     /gene="rutR"
                     /locus_tag="ACHKIR_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HFO9992017.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            6942..8033
                     /gene="rutA"
                     /locus_tag="ACHKIR_004368"
     CDS             6942..8033
                     /gene="rutA"
                     /locus_tag="ACHKIR_004368"
                     /EC_number="1.14.99.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HFO9992018.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDSLTEYVQVLRDLWGSGKS
                     DFKGDFFTMDDCRVSPQPSVPMKVICAGQSDAGMAFSARYADFNFCFGKGVNTPTAFA
                     PTAARMKQAAEQTGRDVGSYVLFMVIADETDDAAHAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSDTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIENFGERIQPLMQCRAHLPALTQEVA"
     gene            8033..8725
                     /gene="rutB"
                     /locus_tag="ACHKIR_004369"
     CDS             8033..8725
                     /gene="rutB"
                     /locus_tag="ACHKIR_004369"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HFO9992019.1"
                     /translation="MTTLTARPEAITFDPQQTALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELMPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSSTSFARIA"
     gene            8737..9123
                     /gene="rutC"
                     /locus_tag="ACHKIR_004370"
     CDS             8737..9123
                     /gene="rutC"
                     /locus_tag="ACHKIR_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-aminoacrylate deaminase"
                     /protein_id="HFO9992020.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFCIQCGLVKPDALVEIATIAHIAK"
     gene            9131..9931
                     /gene="rutD"
                     /locus_tag="ACHKIR_004371"
     CDS             9131..9931
                     /gene="rutD"
                     /locus_tag="ACHKIR_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HFO9992021.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLVQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIERYAVVGHALGALVGMQLALDYP
                     ASVTVLVSVNGWLRINAHTRRCFQVREQLLHSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPTACSS
                     ELHAALPDSQKMVMRYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            9941..10531
                     /gene="rutE"
                     /locus_tag="ACHKIR_004372"
     CDS             9941..10531
                     /gene="rutE"
                     /locus_tag="ACHKIR_004372"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HFO9992022.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLREIYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPQLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQYVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            10542..11036
                     /gene="rutF"
                     /locus_tag="ACHKIR_004373"
     CDS             10542..11036
                     /gene="rutF"
                     /locus_tag="ACHKIR_004373"
                     /EC_number="1.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent FMN reductase RutF"
                     /protein_id="HFO9992023.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHATPYGLVWFDRSYHALM
                     RPAC"
     gene            11057..12385
                     /gene="rutG"
                     /locus_tag="ACHKIR_004374"
     CDS             11057..12385
                     /gene="rutG"
                     /locus_tag="ACHKIR_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015218 - pyrimidine nucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006864 - pyrimidine nucleotide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HFO9992024.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGHVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKNVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNSVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(12468..12641)
                     /gene="ymdF"
                     /locus_tag="ACHKIR_004375"
     CDS             complement(12468..12641)
                     /gene="ymdF"
                     /locus_tag="ACHKIR_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFO9992025.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            13410..13483
                     /locus_tag="ACHKIR_004376"
                     /pseudo
     CDS             13410..13483
                     /locus_tag="ACHKIR_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813257.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
     gene            complement(13573..>13878)
                     /locus_tag="ACHKIR_004377"
     CDS             complement(13573..>13878)
                     /locus_tag="ACHKIR_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001412991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992026.1"
                     /translation="DQQKMYDRMTEVAQYHAELKSLTGAERTAFIDENNGKLSMNGLM
                     QDTRKRLKDLRKQRDAIYADSSLSLAQQVAMVKSVERDMKIAVDRFNREYNKKVGVD"
BASE COUNT         3170 a         3751 c         3677 g         3280 t
ORIGIN      
        1 ccattcaatt tatatggcga ggcacattaa gggagcgcaa cccgctgctg aagccaaaca
       61 aaaccgccgc aggctaagtc ccttaccctt cctgcaaccg tgacggcgga gccgaatgat
      121 agtgcgcatc ggcctcggca aagcgttttt gcatcgccgc tgacggcgct ttacccagca
      181 aactaaacac tacaatcccg atactgccga agataaagcc cggaataatt tcgtacagac
      241 ccagccagcc gaactgtttc cagacgataa ccgtcagcgc accgatgatc atccccgcca
      301 gcgcaccgtt gcgcgtcatg cgtgaccaca tcaccgagaa cagcaccacc ggaccaaacg
      361 ctgcgccaaa gcctgcccac gcgtagctca ctaagcccag cacgcggttt tccgggtttg
      421 ccgccagcgc aatcgccacc agcgccacca ccagcaccat cactcgccct acccacacca
      481 gctctttctg gctggcctgt ttacgcagaa acgctttgta taaatcttcg gtaatcgcac
      541 tggaacatac cagcagctgg caacttaacg ttgacattac cgccgccaga atcgccgaca
      601 gcaaaatccc ggcaatccac gggttaaaca gaatttgcgc cagttcaata aacacgcgtt
      661 cagcgttctg gtttaccgca cctgctaccg ccgggtgctc gttaaagtaa gcgataccaa
      721 agaagccgac agccaccgcc cctgccaggc agaggatcat ccaggtcata ctgatacgac
      781 gcgcatggac aatgctgtgg tgagaatctg ccgccataaa acgcgccagg atgtgcggct
      841 gcccgaagta acccagcccc caccccatca gcgagataat ggcgacaaaa ttcaaccctt
      901 tgagcatatc cacgttttcg atgctctttt gtttgattac ttccagcgag tcgccaaacc
      961 caccgacgct aatgatcacg ataaccggcg tcaggatcag ggcaaaaatc atcaggctgg
     1021 cctgtacagt gtcagtccag ctcaccgcga ggaaaccgcc aataaaggtg taaaggatcg
     1081 tcgccgcagc ccccgcccac agagccgttt cgtagctcat gccaaaggta ctttcaaaca
     1141 gacgcgcgcc tgccacaatg cccgaagcgc aataaatggt gaagaacagc aaaataacca
     1201 gcgcggagat aatgcgcaaa atgcggcttt tatcttcaaa gcgcccggtg aaataatccg
     1261 gcaatgttaa ggcgttattg ttgtattcgg tatgcacacg caaccgcccg gccaccagct
     1321 tccagtttat ccacgcgcct aatgtcaggc caatggcgat ccagctttcg gaaatcccgg
     1381 aaagaaaaac cgcgcccggc aaccccatta acagccagcc gctcatatcc gacgctcccg
     1441 ccgataatgc cgtcacgaat ggcccaagac tacgaccgcc cagaatatag tcgtcgaagt
     1501 ttttcgttga tcgccaggcg ataaacccaa tcaatatcat gccaaagata tagacacaaa
     1561 atgtcaccaa catcggtgtg ctaatagcca tctaaagtct ccaaaaaatt attatcggca
     1621 atgtcgaaac ttgccgttat atctgccacc ggaacggggt aacagagttt atgttttacc
     1681 agggcgaccg tatcctgccg gaagcgctgg ttattcacaa tcgatttaac acaccattta
     1741 cattaaattt tagtgctcag cgacactatt tttcatcagg ttgcactctc tcacattttt
     1801 tgcggttgca cctttcaaaa atgttaactg ccgcagagaa aaagtctgag ttattttttt
     1861 aatccctgtc atatcgattt cgtttattaa catttcattc atttttaagc ttgctacgca
     1921 ggtcacattt aacacagttg cacaaagttg caacatcatg gatatttcac gataacgtta
     1981 agttgcacct ttcagaacaa caggagtaat ggcatgggaa ccaccaccat gggggttaag
     2041 ctggacgacg cgacgcgtga gcgtattaag tctgccgcga cacgtatcga tcgcacgcca
     2101 cactggttaa ttaaacaggc gattttttct tatcttgaac aactggaaaa cagcgatact
     2161 ctgccggagc tacctgcgct gctttctggc gctgccaatg agagcgatga agcaccgact
     2221 ccggcagagg aaccacacca gcctttcctc gactttgccg agcaaatttt gccccagtcg
     2281 gtttcccgcg ccgcgatcac cgctgcctat cgccgtccgg aaaccgaagc ggtttcgatg
     2341 ctgctggaac aagctcgcct gccgcagcca gttgctgaac aggcgcacaa actggcgtat
     2401 caactggccg ataaactgcg taatcaaaaa aacgccagtg gtcgcgcagg tatggttcag
     2461 gggttattgc aggagttttc gttgtcatcg caggaaggcg tggcgctgat gtgtctggca
     2521 gaagcgttgt tgcgtattcc cgacaaagcc acccgtgatg cgttaattcg cgacaaaatc
     2581 agcaacggta actggcagtc acacattggt cgtagcccgt cgttgtttgt taatgccgcc
     2641 acctgggggc tgctgtttac cggcaaactg gtttccaccc ataacgaagc cagcctctcc
     2701 cgctcgctga accgcattat cggtaagagc ggtgaaccgc tgatccgcaa aggtgtggat
     2761 atggcgatgc gcctgatggg cgagcagttc gtcacgggtg aaaccattgc agaagcgtta
     2821 gccaatgcac gcaagctgga agagaaaggt ttccgttact cctacgatat gctgggcgaa
     2881 gccgcgctga ccgccgcaga tgcacaggcg tatatggttt cctatcagca ggcgattcac
     2941 gccattggta aagcgtccaa tggtcgtggc atctatgaag gtccgggcat ttcaatcaag
     3001 ctgtcggcgc tgcatccgcg ttacagccgc gcccagtatg accgggtaat ggaagagctt
     3061 tacccacgtc tgaaatcact caccctgctg gcgcgtcagt acgatattgg tattaacatc
     3121 gatgccgaag aggccgatcg tctggagatc tccctcgatc tgctggaaaa actctgtttc
     3181 gagccggaac tggcaggctg gaacggcatc ggttttgtta ttcaggctta tcaaaaacgc
     3241 tgcccaccgg tgattgatta cctgattgat ctcgccaccc gcagccgtcg ccgtctgatg
     3301 attcgcctgg tgaaaggcgc gtactgggat agtgaaatca agcgcgcgca gatggacggc
     3361 cttgaaggtt atccggttta tacccgcaag gtgtataccg acgtttcata tctcgcctgt
     3421 gcgaaaaagc tgctggcggt gccgaatcta atctacccgc agttcgcaac gcataacgcc
     3481 catacgctgg cggcaattta tcaactggcg ggtcagaact actacccggg tcagtacgag
     3541 ttccagtgcc tgcacggtat gggcgagcca ctgtatgagc aggtcaccgg gaaagttgcc
     3601 gacggcaaac ttaaccgtcc gtgtcgtatt tatgctcctg tcggtaccca tgaaacgctg
     3661 ctggcgtatc tggtgcgtcg cctgctggaa aacggtgcta acacctcgtt tgttaaccgt
     3721 attgctgaca cctctttgcc actggatgaa ctggttgccg atccggtcac tgctgtagaa
     3781 aaactggcgc aacaggaagg gcaaactgga ttaccgcatc cgaaaattcc cctgccgcgc
     3841 gatctttacg gtgacgggcg cgacaactcg gcagggctgg atctcgccaa tgaacaccgg
     3901 ctggcctcgc tctcttctgc cctgctcaat agtgcactgc aaaaatggca ggccttgcca
     3961 atgctggaac aatcggtcgc ggctggtgag atgtcgcccg ttattaaccc tgcggaaccg
     4021 aaagatattg tgggctttgt gcgtgaagcc acgccgcgtg aagtagaaca ggcgctggaa
     4081 agtgcggtta ataacgcgcc aatctggttt gccactcctc cggctgaacg cgcggcgatt
     4141 ttgcatcgcg ctgccgtgct aatggaaagc cagatgcagc aactgattgg tattctggtg
     4201 cgtgaagccg gaaaaacctt cagtaacgcc attgccgaag tgcgcgaagc ggtcgatttt
     4261 ctccactact atgccggaca ggtgcgggat gatttcgcta acgaaaccca ccgtccatta
     4321 gggcctgtgg tatgtatcag tccgtggaac ttcccgctgg ctattttcac cgggcagatc
     4381 gctgccgcac tggcggcagg taacagcgtg ctggcaaaac cggcagaaca aacgccgctg
     4441 attgccgcgc aagggatcgc cattttgctg gaagcgggtg taccgccagg cgtggtgcaa
     4501 ttgctgccag gtcggggtga aaccgtgggc gcgcaactga cgggtgatga tcgcgtgcgc
     4561 ggggtgatgt ttaccggttc aaccgaagtc gctacgttac tgcagcgcaa tatcgccagc
     4621 cgcctggacg ctcagggtcg ccctattccg ctcatcgctg aaaccggcgg catgaacgcg
     4681 atgattgtcg attcttcagc actgaccgaa caagtcgtaa tagatgtgct ggcctcggcg
     4741 ttcgacagtg caggtcagcg ttgttcggca ctgcgcgtgc tgtgcctgca agatgagatt
     4801 gccgaccaca cgctgaaaat gctgcgcggc gcaatggccg aatgccggat gggtaatccg
     4861 ggtcgcctga ccaccgatat cggtccggtg attgatagcg aagcgaaagc caatattgag
     4921 cgtcatattc agaccatgcg tagtaaaggc cgtcaggtgt tccaggcggt gcgggaaaac
     4981 agcgaagatg cccgtgaatg gcaaagcggc acctttgtcg ctccgacgct gatcgaactg
     5041 gatgactttg ccgaattaca aaaagaggtc tttggtccgg tgttgcatgt ggtgcgttac
     5101 aaccgtaacc agctgccggc gctgatcgag cagattaacg cttccggtta tggtctgacg
     5161 cttggcgtcc atacgcgcat tgatgaaacc atcgcccagg tcactggctc ggcgcatgtc
     5221 ggtaacctgt acgttaaccg taatatggtg ggcgcagtgg ttggggtgca accgttcggc
     5281 ggcgaagggt tgtccggtac cgggccgaaa gcaggcggtc cgctctatct ctaccgtctg
     5341 ctggcgaatc gcccggaaag tgcgctggca gtgacgctcg cgcgtcagga tgcggagtat
     5401 ccggtcgatg cgcagttgaa agccgcattg actcagccgc taaatgcact gcgggaatgg
     5461 gcggcaaacc gtccagaatt gcaggcgtta tgtacgcaat atggcgagtt ggcgcaggca
     5521 ggaacacaac gattgttgcc agggccgacg ggtgaacgca acacctggac gctgctgccg
     5581 cgtgagcgcg tgttgtgtat tgcggatgat gaacaggatg cgctgactca gctcgccgcc
     5641 gtgctggcgg tgggtagcca ggtgctgtgg ccggatgatg cgctgcatcg tcagttagtg
     5701 aaggcattgc catcggcagt cagcgaacgt attcaactgg cgaaagcgga aaatataacc
     5761 gctcaaccat ttgatgcggt gatcttccac ggtgattcgg atcagcttcg cgcattgtgt
     5821 gaagccgttg ccgcgcggga tggtgcaatt gtttcggtgc agggttttgc ccgtggcgaa
     5881 agcaatatcc ttctggaacg gctgtatatc gagcgttcgc tgagtgtgaa taccgctgct
     5941 gctggcggta acgccagttt aatgacgatc ggttaaacag gtagccggag gatgttacaa
     6001 cctcctccgg catctttaac gtggtcgaat cccctcaata ataatccgct gcacgttttc
     6061 aaccgtttga ttgaaaaaca cctcatcgcg caacgtcgcg cctgtcaccg cctccacctg
     6121 aggggcaaaa tcggcgtaat gttgagtgga agcccaaatc ataaaaatca aatgctgcgg
     6181 gtcaatcggc gcgagtttgc cgcttttaac ccaaccggca atgagcgccg atttctcatc
     6241 aattaatgcc ttcaaatcgc ccgtcagttc atccattaac agcggtgcac ctgccagcat
     6301 ctccatacag aacaggcgcg aagcctgcgg ataatcgcgg gaaacttcca gcttcagacg
     6361 gatgtattct ttgatcgccg ccagcggggc gaaatcttct cgaaacgctt ttaacggtgc
     6421 cagccagata tcgagaatct gccgcagtac ggctatatac agcgcctctt ttgacggaaa
     6481 gtaatacagc agattggttt ttgaaacacc cgccaactcc gcgatctgct ccagccttgt
     6541 gccgtgaaaa ccgaattgtg aaaaagtgtc cagtgctgcg ctaagaatcg cttttttctt
     6601 cgcgcttact gcgcgcgaac gcttacccgt tgttttcact gcgccttgcg tcatgcgctc
     6661 tcccctcttg ttgattggca acaggatagc aaaacccccg cccggcctcg accttctgca
     6721 ctctcatcgc gcacgaatgc atgttttatg tgcaactgtt ttgaccgttt agtccacttt
     6781 ttttcaggca ttttagacaa cttaacatta acacattaat aacattcact ttttaaaaac
     6841 tggcatccgc tttgcaaaca agccattacg cggctgaaaa ggatatgcag gatgcagcgc
     6901 cccgtctgac tttcacttta cgagacgaag agaggttgat gatgaaaatt ggcgtattcg
     6961 tacctattgg caacaacggc tggctcattt cgacccacgc gccgcagtac atgccgacat
     7021 ttgaactgaa taaagccatt gtgcaaaaag cggagcacta ccatttcgat ttcgccctgt
     7081 cgatgatcaa actgcgtggc tttggcggca aaactgagtt ctgggatcac aaccttgagt
     7141 cgttcacctt gatggcgggg ctggcagccg tcacctcacg cattcagata tacgcgacag
     7201 ctgccacctt aacgttacct ccggcaatcg tcgcccgtat ggccgcaacc atcgactcca
     7261 tctctggcgg gcgttttggc gtcaacctcg tgactggctg gcagaagccc gagtatgagc
     7321 agatggggat ctggcctggc gatgactatt tctcccgtcg ttacgactct ctcaccgaat
     7381 atgttcaggt gctgcgcgac ctgtggggct cggggaaaag cgattttaaa ggcgattttt
     7441 tcactatgga tgattgtcgc gtcagtccgc aaccgagtgt ccccatgaaa gtgatctgcg
     7501 ccgggcaaag cgacgctggc atggcgttct ccgcccggta tgccgatttt aacttctgtt
     7561 tcggcaaagg cgtaaataca cccacggctt tcgccccgac cgctgcgcgg atgaaacagg
     7621 ccgcagagca aaccggacgc gacgttggct cttatgtgtt gtttatggtg attgccgacg
     7681 aaaccgacga tgccgctcat gcaaaatggg aacactacaa agcgggcgcg gatgaagagg
     7741 cgttaagctg gctaaccgaa caaagtcaga aagatacccg ctccgatact gacaccaacg
     7801 tccgtcagat ggccgatccc acttcggcgg taaacatcaa tatggggacg ttagtcggtt
     7861 cttacgccag tgtcgcgcgc atgttagatg aagtcgcaag cgtgcctggt gccgaaggcg
     7921 tgctgttgac cttcgatgat tttctgtcgg gaatcgaaaa cttcggcgag cgcattcaac
     7981 cactgatgca gtgccgcgcc catctccctg cgctgactca ggaggtggca tgatgacgac
     8041 cttaaccgca cgaccggaag ccattacctt cgatccgcag caaactgcgc tgatcgtggt
     8101 ggatatgcaa aacgcttatg ccacgccagg cggctactta gatctcgccg ggtttgatgt
     8161 ctcaaccact cgcccggtca ttgccaacat tcaaaccgcc gtgaccgcag cgcgagcggc
     8221 agggatgttg atcatctggt ttcaaaatgg ctgggatgaa cagtatgtcg aggctggcgg
     8281 ccccggctca ccgaattttc ataaatcgaa cgccctgaaa accatgcgta agcagccgca
     8341 gctgcagggg aaattgctgg cgaaaggctc ctgggattat caactggtgg atgaactgat
     8401 gccgcagcct ggcgatattg tgctgccgaa gccgcgctac agcggtttct tcaatacgcc
     8461 gctggacagc attttgcgca gccgtggaat acgccatctg gttttcaccg gtatcgctac
     8521 caacgtctgc gtcgaatcga cgctacgcga cggctttttt ctggagtatt tcggcgtggt
     8581 gctggaagac gcaacacacc aggcgggacc ggaatttgca cagaaagccg cgttgttcaa
     8641 tatcgaaacc ttttttggct gggtcagcga cgtcgaaacg ttctgcgacg cgctttcttc
     8701 cacgtccttt gctcgtatcg cttaaggagt ttaacgatgc caaaatccgt aattattcct
     8761 gctggcagca gcgcaccgct ggcccccttc gttcccggca cgctggctga tggcgtggtg
     8821 tatgtctccg gtacgctggc ttttgatcaa cataacaacg tgctgtttgc cgatgaccca
     8881 aaggcgcaaa cccgccacgt tctggaaacc atccgcaagg tcatcgagac ggcgggtggc
     8941 acaatggcgg atgtgacctt caacagcatc tttattaccg actggaaaaa ttacgccgcg
     9001 attaacgaaa tctacgccga atttttcccg ggtgacaaac cggcgcgatt ctgcattcag
     9061 tgcggactgg taaaacctga cgcgctggtg gaaatcgcca caattgcgca tatcgccaag
     9121 tgaggccgcg atgaaacttt cactctcacc tcccccttat gctgatgcgc ccgtagtggt
     9181 gttgatttcg ggtcttggcg gtagcggcag ttactggtta ccgcaactgg cggtgttggt
     9241 tcaggagtat caggtagtct gttacgacca gcgcggcacc ggcaataatc ccgacacgct
     9301 ggcagaagat tacagtatcg cccagatggc agcggaactg catcaggcgc tggtagccgc
     9361 agggattgag cgttacgcgg tggtcggcca tgcgctcggt gcgctggtgg gaatgcagct
     9421 ggcgctggat tatcccgcgt cggtaactgt gctggtcagc gttaacggct ggctacgaat
     9481 aaacgcccat acgcgccgct gttttcaggt tcgcgaacag ttactacata gcggcggcgc
     9541 gcaggcatgg gtggaagcgc agccgttgtt cctctatccc gccgactgga tggcggcccg
     9601 cgcacctcgc cttgaggcag aagacgcgct ggcactggcg cattttcaag gcaaaaataa
     9661 tttactgcgt cgacttaacg ccctcaaacg cgctgacttt agtcaccatg cggatcgtat
     9721 ccgctgcccg gtgcaaatca tctgcgccag tgatgatctg ctggtgccaa cagcatgttc
     9781 cagtgaactt catgccgccc tgcccgatag ccagaaaatg gtgatgcgct atggcggaca
     9841 cgcctgcaac gtgaccgatc ccgaaacgtt taatgctctg ttactcaacg ggcttgccag
     9901 cctgttacat caccgtgaag ccgctctgta aggaattgct atgaacgaag ccgttagccc
     9961 aggtgcgctt agcaccctgt tcaccgatgc ccgcactcac aacggctggc gggagacacc
    10021 cgtcagcgat gagacgttac gggagattta tgccctgatg aaatgggggc cgacatcagc
    10081 taactgttct ccggcacgga tcgtgtttat ccgcactgca gaaggaaaag aacgtctgcg
    10141 cccggcgctt tccagcggca atctgcaaaa aaccctgacc gcgcccgtca ccgctatcgt
    10201 cgcctgggac agtgaatttt atgaacggtt accacagctg tttcctcacg gcgatgcccg
    10261 cagttggttt acctccagtc cacaacttgc cgaagaaaca gcgtttcgca acagttccat
    10321 gcaggcggcc tatctgatcg tcgcctgccg tgcgctgggg ctggataccg gcccgatgtc
    10381 gggctttgac cgtcaatacg ttgacgacgc cttttttgcg ggcagcacgc tgaagagcaa
    10441 tctgctgatt aatatcggct atggcgattc cagcaaactt tttgcgcgcc tgccacgtct
    10501 gtcctttgaa gaagcctgcg ggctgttgta aggagcaaac aatgaacatt gtcgatcaac
    10561 aaacctttcg cgatgcgatg tcctgtatgg gcgcggcggt caatatcatc actacggacg
    10621 gtccagccgg gcgcgccggg ttcaccgcca gcgccgtctg cagcgtgact gacacgccgc
    10681 ccacattact ggtgtgcctg aatcgtgggg cgtccgtctg gccggtattc aatgaaaatc
    10741 gaacgctgtg tgtaaataca cttagcgccg ggcaggagcc gctttcaaac ctttttggcg
    10801 gcaaaacgcc catggaacac cgctttgccg ccgcccgctg gcagactggt gtgaccggat
    10861 gcccgcaact ggaagaggca ctggtttcgt ttgactgccg tatcagccag gtggtgagcg
    10921 tcggcaccca cgacattttg ttttgcgcca tcgaagcgat tcatcgtcac gccacaccct
    10981 acgggctggt gtggttcgat cgcagttatc acgcgctgat gcgccctgct tgttaacctc
    11041 cttaaggaga cagctgatgg caatgttcgg ttttcctcac tggcagttaa aatcgacctc
    11101 tacagagagc ggcgtggtcg cgccggatga acgattgccg tttgctcaaa cggcagtaat
    11161 gggcgttcaa cacgcggtgg cgatgtttgg cgcgacggtg ttaatgccaa ttctgatggg
    11221 gctggatccc aatctttcca ttttaatgtc ggggatcggc acgttactgt ttttctttat
    11281 taccggcggg cacgtgccca gttatctcgg ttccagcgcg gcttttgtcg gcgtggtgat
    11341 tgccgcgacg ggctttaatg gtcagggaat taatccgaat atcagcatcg cgctgggcgg
    11401 gattatcgcc tgtgggctgg tttataccgt cattggcctg gtggtgatga aaatcggtac
    11461 gcgctggatt gaacgactaa tgccaccggt tgtgacaggt gcggtagtga tggcgattgg
    11521 cctgaactta gcgcctatcg cagtgaaaaa tgtatcggct tcggcctttg atagctggat
    11581 ggcggtaatg acggtgctgt gtattgggct ggtggccgta tttacccgtg gcatgatcca
    11641 gcgattattg attctcgtag gattaattgt cgcctgtctg ctgtatggcg tgatgaccaa
    11701 cgttttaggg ctgggaaaag ccgtcgattt tactctcgtc agccatgctg cctggttcgg
    11761 tctgccacat ttttccactc ctgcttttaa tggtcaggca atgatgctga ttgcgcccgt
    11821 ggcggtgatt ctggtggcgg aaaacttagg tcatctcaag gcggtcgccg ggatgaccgg
    11881 acgcaatatg gacccgtata tggggcgggc attcgtaggc gatgggctgg cgacgatgct
    11941 ttctggctct gtcggcggta gcggcgtaac cacctacgcc gagaatattg gtgtgatggc
    12001 cgtgacaaaa gtctactcga cgctggtgtt tgttgccgct gccgtcatcg ccatgctgtt
    12061 gggcttttcg ccgaaatttg gcgcgctgat ccatactatt ccggccgcgg tgattggtgg
    12121 tgcatcaatt gttgtgttcg ggctgattgc cgtcgcaggc gcacggatct gggtacaaaa
    12181 cagtgtggat ttaagccaga acggtaattt gattatggtc gcagtaacct tagtgctggg
    12241 tgcgggtgat tttgctctca cactgggcgg ttttacgttg ggagggatcg gtacagccac
    12301 ctttggcgcg attttactca atgcgttgtt aagcagaagg ttggttgacg ttccaccgcc
    12361 agaagttgtt catcaggagc cataaataga aaaccgccgg ggtaaccggc ggtgatgact
    12421 ttttgacgag tttcagacaa aaatacattc gtcatggatt agcccggcta gttgtcgctt
    12481 ttaccgtgac tgctcttgcc tcctttttta cctgcttcag atgcgcgctg cggatcattt
    12541 ttgaaattac ccccgctgtg ctgtccacct tttttacctg cttctgatgc tctttcgcgg
    12601 tcttctgcaa aattgccgga accgcctcga tggtttgcca ttactgacct ccgtctgggt
    12661 atagacatca tattgcatag aattaacgcg gcgtatggcg gctgccatat tttcgctacg
    12721 tgggactaag cttagtgaac tgacgcgggc agacccgcgt ttagtaatat cctgcaacag
    12781 cttcttagcg agaatagata tcatctccaa taattatcca taagccgctc ttatgattaa
    12841 aaaaacgcga tgagataaaa atgtgtcatt ttgcgacaaa attacgtgct tgtgaaagtt
    12901 gttataaatc aaataagtgg ttgtgaaatt tgcactctga aaaggacgtc ttatctttaa
    12961 ataagtggta gcgaatcgct acggaataga gataacacga ggagtggtta gaaatggcta
    13021 aagttctggt gctttattat tccatgtacg gacatattga aaccatgaag cattaaaaac
    13081 acagaattac cgaagatcgt ccaagagcat atacttttca atatgttaga cctcattttg
    13141 accataaaga aatcgatttt acaccgttct ttatacgttc ttttttgccc caaatatgcc
    13201 ccaaacaacc acactttgcc ccaatagcgc cccaaagatt taaggggatt taagtatata
    13261 tttcagataa ttaacgcaag tttacgatgt gaatttacgt taattttctg ggtagggtat
    13321 ttaccgtcat tcgttcatca atatttctgc atattgacaa atacgcctga gctatcgcgg
    13381 gagaaatgca atgaaagcag caaaaggcga tgttaatcac cctgctcatc tctgtattac
    13441 cgtcattgtg acggcactga taacgaagaa agacctctgc aagggaggaa tccctcgcca
    13501 cctctgatgt gtcaggtatc ctcaccctcc ccaatcacat tagccccgca cttccgtacg
    13561 gggccatttc tgttaatcca ctcccacttt tttgttgtac tcgcggttaa accgatccac
    13621 ggcaattttc atatcccgct ctaccgattt caccatcgcc acctgctgcg ccagactgag
    13681 agaactgtcg gcataaatgg catcgcgctg tttacgcaaa tccttcagtc tcttccgggt
    13741 atcctgcata agcccgttca tcgacaattt tccgttgttc tcgtcaatga acgccgttct
    13801 ttctgcaccg gtcagactct tcagctctgc gtggtactgc gcaacctccg tcatccggtc
    13861 gtacatcttc tgctggtc
//
LOCUS       DAZGQL010000123        13846 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.123, whole genome shotgun sequence.
ACCESSION   DAZGQL010000123 DAZGQL010000000
VERSION     DAZGQL010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13846
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.123"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..187
                     /locus_tag="ACHKIR_004378"
     CDS             <1..187
                     /locus_tag="ACHKIR_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HFO9992027.1"
                     /translation="ELGVPASAADITARADQEGWNPGFTEKMVGWAKKMESGERSVIK
                     NPEYFSTYMQEELKALV"
     gene            complement(307..486)
                     /gene="yoaH"
                     /locus_tag="ACHKIR_004379"
     CDS             complement(307..486)
                     /gene="yoaH"
                     /locus_tag="ACHKIR_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HFO9992028.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HAGERIVARFEDEDE"
     gene            560..1921
                     /gene="pabB"
                     /locus_tag="ACHKIR_004380"
     CDS             560..1921
                     /gene="pabB"
                     /locus_tag="ACHKIR_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HFO9992029.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVAEPICTLTTFGKETVVSENEKRTTTTDDPLQVLQQVLDRADIRPAHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAQQDIVLPDMAVGIYDWALVVDHQRQTVSLLSHNDV
                     NARRAWLESQQFSPQEGFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHTSDLLRAAFPGGSITGAPKVRAMEIIDELEPHRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            1924..2502
                     /gene="nudL"
                     /locus_tag="ACHKIR_004381"
     CDS             1924..2502
                     /gene="nudL"
                     /locus_tag="ACHKIR_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HFO9992030.1"
                     /translation="MEHRSLTLDDFLSRFQLLRPQMNRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            2686..4050
                     /gene="sdaA"
                     /locus_tag="ACHKIR_004382"
     CDS             2686..4050
                     /gene="sdaA"
                     /locus_tag="ACHKIR_004382"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFO9992031.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            4181..5779
                     /gene="pdeD"
                     /locus_tag="ACHKIR_004383"
     CDS             4181..5779
                     /gene="pdeD"
                     /locus_tag="ACHKIR_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFO9992032.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTTDHALIKGSPVLIQWTPAAGSSK
                     AGVMEMINIDLLAAMLLEPQLPQISSASLTVDDRHLLYGNGLVDSLPQPENNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLLRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(5783..7339)
                     /gene="yoaE"
                     /locus_tag="ACHKIR_004384"
     CDS             complement(5783..7339)
                     /gene="yoaE"
                     /locus_tag="ACHKIR_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HFO9992033.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPMPI
                     PEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLF
                     PVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRAR
                     GSFVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQ
                     QALDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIV
                     KEQPAHDEDE"
     gene            complement(7327..7485)
                     /gene="yoaL"
                     /locus_tag="ACHKIR_004385"
     CDS             complement(7327..7485)
                     /gene="yoaL"
                     /locus_tag="ACHKIR_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaL"
                     /protein_id="HFO9992034.1"
                     /translation="MDRHRRHFSIRPFNACLSGTLCRTFRLHFVVTPALFLASNSYSL
                     SRSLSWNS"
     gene            7802..8773
                     /gene="manX"
                     /locus_tag="ACHKIR_004386"
     CDS             7802..8773
                     /gene="manX"
                     /locus_tag="ACHKIR_004386"
                     /EC_number="2.7.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HFO9992035.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKATPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            8836..9636
                     /gene="manY"
                     /locus_tag="ACHKIR_004387"
     CDS             8836..9636
                     /gene="manY"
                     /locus_tag="ACHKIR_004387"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HFO9992036.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            9649..10500
                     /gene="manZ"
                     /locus_tag="ACHKIR_004388"
     CDS             9649..10500
                     /gene="manZ"
                     /locus_tag="ACHKIR_004388"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HFO9992037.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            10555..11013
                     /gene="yobD"
                     /locus_tag="ACHKIR_004389"
     CDS             10555..11013
                     /gene="yobD"
                     /locus_tag="ACHKIR_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 family protein"
                     /protein_id="HFO9992038.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLVSTQ"
     gene            11443..12009
                     /gene="mntP"
                     /locus_tag="ACHKIR_004390"
     CDS             11443..12009
                     /gene="mntP"
                     /locus_tag="ACHKIR_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HFO9992039.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDKEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     gene            complement(12006..12815)
                     /gene="rlmA"
                     /locus_tag="ACHKIR_004391"
     CDS             complement(12006..12815)
                     /gene="rlmA"
                     /locus_tag="ACHKIR_004391"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HFO9992040.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTFCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSDELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(12981..13190)
                     /gene="cspE"
                     /locus_tag="ACHKIR_004392"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(12981..13190)
                     /gene="cspE"
                     /locus_tag="ACHKIR_004392"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFO9992041.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            complement(13203..13346)
                     /gene="yobF"
                     /locus_tag="ACHKIR_004393"
     CDS             complement(13203..13346)
                     /gene="yobF"
                     /locus_tag="ACHKIR_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2627 domain-containing protein"
                     /protein_id="HFO9992042.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
BASE COUNT         3446 a         3472 c         3419 g         3509 t
ORIGIN      
        1 agaactggga gtacccgcga gtgccgctga tattacggcg cgagctgacc aggaaggctg
       61 gaaccctggt ttcacggaaa aaatggttgg atgggcaaaa aaaatggagt ctggtgaacg
      121 ctctgtgatt aaaaatcctg aatacttttc aacatatatg caggaagaac taaaagcact
      181 ggtttgagtt aaatttacat cagcataaat gggtcaggac gcttttaatc acatataaaa
      241 agcgtccttt ttctcacaat caaataatca tcggcttcta taacgaaagc gatacggatt
      301 agtgtgttac tcgtcttcat cctcaaaacg cgccacgatt cgctcgccgg catggttggc
      361 acgcagctct tctgccacca acgcgattgc ctggccgctg ctcatccctt gagccatcag
      421 ttcctggatc cgctcgacag ctttttgctg ctgttcatgg gtgagtgaag gtaaacctgc
      481 aaacattgtt aactcctgct aaattgttgg cgctaattat ttcatgctac ccggcacata
      541 gccagtagag tcaggactga tgaagacgtt atctcccgct gtgattactt taccctggcg
      601 tcaggacgcc gctgaatttt atttctcacg cttaagccac ctgccgtggg cgatgctttt
      661 acactccggc tatgctgatc atccatatag ccgctttgat attgtggtcg ccgagccgat
      721 ttgcacttta accactttcg gtaaagaaac cgttgttagt gaaaacgaaa aacgcacaac
      781 gaccactgat gacccgctac aggtgctcca gcaggtgctg gatcgcgcag acattcgccc
      841 agcgcataac gaagatttgc catttcaggg cggcgcgctg gggttgtttg gctacgatct
      901 gggccgccgt tttgagtcac tgccagaaat tgcgcagcaa gatatcgttc tgccggatat
      961 ggcagtgggt atctacgact gggcgctggt tgttgaccac cagcgtcaaa cagtttcttt
     1021 gctgagtcat aatgatgtca atgctcgtcg ggcctggctg gaaagccagc aattctcgcc
     1081 gcaggaaggt ttcacgctca cttccgactg gcaatccaat atgacccgcg agcagtacgg
     1141 cgaaaaattt cgccaggtac aggaatatct gcacagcggt gattgctatc aggtgaatct
     1201 cgcccagcgt tttcatgcga cctattctgg cgatgaatgg caggcattcc ttcagcttaa
     1261 tcaggccaac cgcgcgccat ttagcgcttt tttacgtctt gaacagggtg caattttaag
     1321 cctttcgcca gagcggttta ttctttgtga taatagtgaa atccagaccc gcccgattaa
     1381 aggcacgcta ccacgcctgc ccgatcctca ggaagatagc aaacaagcag aaaaactggc
     1441 gaactcagcg aaagatcgtg ccgaaaatct gatgattgtc gatttaatgc gtaatgatat
     1501 cggtcgcgtt gccgtagccg gttcggtaaa agtaccagag ctgttcgtgg tcgaaccctt
     1561 ccctgccgtg catcatctgg tcagcaccat aacggcgcgg ctaccagaac agttacacac
     1621 cagcgatctg ctgcgcgcag cttttcctgg tggctcaata actggggctc cgaaagtacg
     1681 ggctatggaa attatcgacg aactggaacc gcaccgacgc aatgcctggt gcggcagcat
     1741 tggctatttg agcttttgcg gcaacatgga taccagcatt actatccgca cgctaactgc
     1801 cattaacgga caaatatact gctctgcggg cggtggaatt gtcgccgata gccaggaaga
     1861 agcggaatat caggaaactt ttgataaagt taataagatc ctacgtcaac tggagaaata
     1921 aacgtggaac accgtagcct gacgcttgat gattttttat cgcgctttca acttttgcgt
     1981 ccgcaaatga accgggaaac gttaaatcat cgtcaggctg ccgtgttaat ccccatcgtc
     2041 cgtcgaccgc aaccggggtt gttgctgact cagcgttcga ttcatctgcg taaacacgct
     2101 ggacaagtgg cattccctgg aggcgcagtc gatgacacgg acgcatcagt tatcgccgcc
     2161 gcgctgcgcg aagctgaaga agaggtcgct ataccgcctt ccgccgttga agttatcggc
     2221 gtgctgccgc ccgtcgatag cgtaactggc taccaggtaa ccccagtggt cggcattatc
     2281 ccgcccgatc tgccgtatcg cgccagtgaa gatgaagtct cggcggtgtt tgaaatgccg
     2341 ctcgcccagg cattacatct gggtcgttat catcctttag atatctaccg ccgtggcgat
     2401 tcacatcggg tatggctgtc ctggtacgaa cagtattttg tatggggaat gaccgcaggc
     2461 ataattcgtg agctggcgct gcaaattggt gtgaaaccct gactatactt atctttacat
     2521 ctacaaaaca ctacttgaga caatcatcgc aatattagtt aaatcgcggt ttttgattag
     2581 tttaattcat gtgaatagtt aagccagtcg ccgcgttccc tcttacacta tgcgctgtta
     2641 ttagttcgtt actggaagtc cagtcacctt gtcaggagta ttatcgtgat tagtctattc
     2701 gacatgttta aggtggggat tggtccctca tcttcccata ccgtagggcc tatgaaggcg
     2761 ggtaaacagt tcgtcgatga tctggtcgaa aaaggcttac tggatagcgt tactcgcgtt
     2821 gccgtggacg tttatggttc actgtcgctg acgggtaaag gccaccacac cgatatcgcc
     2881 attattatgg gtcttgcagg taacgaacct gccaccgtgg atatcgacag tattcccggt
     2941 tttattcgcg acgtagaaga gcgcgaacgt ctgctgctgg cacagggacg gcatgaagtg
     3001 gatttcccgc gcgacaacgg gatgcgtttt cataacggta accttccgct gcatgaaaac
     3061 ggtatgcaaa tccacgccta taacggcgat gaagtcgtct acagcaaaac ttattattcc
     3121 atcggcggcg gttttatcgt cgatgaagaa cactttggtc aggatgctgc caacgaagtg
     3181 agcgtgccgt atccgttcaa atctgccacc gaactgctcg cgtactgtaa tgaaaccggc
     3241 tattcgcttt ctggtctcgc tatgcaaaac gaactggcgc tgcacagcaa gaaagagatc
     3301 gacgagtatt tcgcgcatgt ctggcaaacc atgcaggcat gtatcgatcg cgggatgaat
     3361 accgaaggcg tactgccagg cccgttgcgc gtgccacgtc gtgcgtcggc cctgcgccgg
     3421 atgctggttt ccagcgataa actgtctaac gatccgatga atgtcattga ctgggtaaac
     3481 atgtttgcgc tggcagttaa cgaagaaaac gccgccggtg gccgtgtggt aactgcgcca
     3541 actaacggtg cctgcggtat cgttccggca gtgctggctt actatgacca ctttattgaa
     3601 tcagttagcc cagacatcta tacccgttac tttatggcag cgggcgcgat tggtgcactg
     3661 tataaaatga acgcgtctat ttccggtgcg gaagtcggtt gccagggcga agtaggtgtt
     3721 gcctgttcaa tggctgctgc gggccttgcc gagctgttgg gcggtagccc ggaacaggtt
     3781 tgtgtggcag cggaaattgg catggagcac aacctcggtc tgacctgcga ccccgttgca
     3841 gggcaagttc aggtgccgtg cattgaacgt aatgccattg cctctgtgaa ggcgattaac
     3901 gccgcacgga tggctctgcg ccgcaccagt gcaccgcgcg tctcgctgga taaggtcatc
     3961 gaaacgatgt acgaaaccgg taaggacatg aacgctaaat accgcgaaac ctcacgcggt
     4021 ggtctggcaa tcaaagtcca gtgtgactaa tacttcttac tcgcccatct gcaacggatg
     4081 ggcgaattta ttcccgcttt ctcgtctgct gtaattttcc ccactacact tccactgttg
     4141 cgtcaggcgt ttgtcgccat acgcttacag ggtggcccgc atgcaaaaag cacaacggat
     4201 cattaaaacc tatcgccgta atcgaatgat tgtttgtacg atttgcgcac tcgttacgct
     4261 cgcttcgacc ctgagcgtgc gatttatttc acagcgtaac ttaaatcaac aacgggtagt
     4321 acaattcgcc aatcacgctg tagaggaatt agataaagta ctgcttcccc tacaggcagg
     4381 tagcgaagtc ttgcttccgc tgattggtct gccctgctct gtcgcccatt tgccattacg
     4441 taaacaggcg gcaaaactcc aaactgtgcg atccattggc ctggtgcaag acggcacact
     4501 ttattgctcc agcatttttg gttatcgcaa tgtgcccgtc gtggacattc tggctgaact
     4561 tcctgcaccg caaccacttt tacgcctgac gaccgaccat gccctgatta aaggcagtcc
     4621 ggttttgatt caatggacgc cagcagcggg cagtagcaaa gctggggtca tggagatgat
     4681 taacatcgac ttactggcgg caatgctgct tgagccacaa ctgccgcaaa tcagtagcgc
     4741 cagcctgacg gtggacgatc ggcatttgct ctatggtaat gggctggtag attcccttcc
     4801 gcaacctgaa aacaatgaaa actatcaggt ttcttcgcaa cgctttcctt ttaccattaa
     4861 cgttaatggt ccgggggcta cggcgctggc atggcactat cttccaacac aattaccgct
     4921 ggcggtgctg ctaagtttac ttgtgggcta tatcgcctgg ctggcgaccg cttaccggat
     4981 gagtttttcc cgcgaaatca atctgggcct ggcgcaacat gagttcgaat tgttctgcca
     5041 gcctttgctt aatgcgcgca gccagcaatg tattggcgta gagattttgc tgcgctggaa
     5101 caatccgcgt cagggctgga tttcgccgga tgtgtttatt cctatcgcgg aagaacatca
     5161 tttaattgtg ccactgaccc gctatgtgat ggcagaaacc attcgtcagc gccatgtttt
     5221 cccgatgagt agtcagtttc atgttggcat taacgtcgca cccagccatt ttcgccgtgg
     5281 tgtgctgata aaagatctca atcagtactg gtttagcgct cacccgattc agcaactgat
     5341 cctcgaaatc accgaacgcg atgccttact ggatgttgat tatcggattg cccgcgagct
     5401 acatcgtaaa aacgtcaaac tggcgattga tgacttcggc accggcaata gttcattttc
     5461 ctggcttgaa acattacgtc ctgacgtgct gaaaattgat aagtcattta ccgcagctat
     5521 aggttctgac gcggttaact cgacggtgac cgatatcatc atcgcactgg ggcaaagact
     5581 gaatattgaa ctggtggcgg agggtgtgga aacgcaagaa caggcgaaat atttgctccg
     5641 tcatggcgtc catattttgc aagggtattt gtacgcacag ccgatgccac tacgtgattt
     5701 tcccaaatgg ctggcgggca gccaaccgcc gcccgcccgg cataatggac atatcacacc
     5761 cattatgccg ttacgttaac gcttactcat cttcatcgtg cgccggttgc tctttaacaa
     5821 tgcgaaccag atcaacacga taatcattgg cttcaatgat ggtgatatgc agtggcccta
     5881 catcaatcac atcgcccaca cggggaatgt gaccatttgc cgagatcacg aggcccgcga
     5941 ccgtcgcgat atcatcgtca tcggcaaggt gctcaacatc aagcgcctgc tgcaaggcat
     6001 gcaaatctgt accgcctttt accagccagc cgtcaccgtc ggtaatgatt tccggcgttt
     6061 cgtcagcgtc cgggaattca cccgcaatgg cttccagcac atccagcggc gtgaccaagc
     6121 cttgtaccac accaaactcg ttggtcacga taacaaagct cccgcgagca cgacgcagca
     6181 cgcccagcag gttgatcgga tcgagggttt ccgggacgat aatcgccgga gacgccgaag
     6241 caatcgccgc cacatcaacg ccctcttcca gcgccaccag cagttcttta gcacgtacga
     6301 ttccgatgat ttcatccagt tcaccgcgac ataccgggaa caggctgtgc ggtgaggaga
     6361 gcagctgctc gcggatttca tctaccccga gattagcgtc aacccagctt atttcaccgc
     6421 gcggcgtcat gatcccgcgc agagaacgcg acgccagcgt cagtacgccg ttaatcatgt
     6481 aacgttcttc ttcggcaaat gcaccttccg ggatcggcat cggcatcggg ttatcggcat
     6541 cgtgctgaac attggcctga cgtttcccgc ccatcaaacg caggatggca tcggcagtac
     6601 gcgctcgcag cggcaaagtc gactggtgac gaataaagtt gcgacgcgca atctggttaa
     6661 acacttcgat gatgatcgag aagccaatcg cggcatacag gtaacctttc ggaatgtgga
     6721 aaccgaaacc ttctgccacc agactcagac caatcattaa caggaagctc agacagagca
     6781 ccaccaccgt ggggtgctgg ttaacaaatc gcgtcagcgg tttcgatgcc agcagcataa
     6841 ctgccatcgc aatcactacc gccgccatca tcaccggcaa atggttaacc atccctactg
     6901 cagtaattac cgcatccaac gagaagacgg cgtcaaggat gacaatctgt gtgacgacca
     6961 cccagaaact ggcgtagcct ttaccgtggc cggaatcatg atcgcggttt tccagccgtt
     7021 catgcagttc ggttgttgct ttgaacagca agaatatgcc cccgaacaac ataatcaggt
     7081 cgcgtccgga gaaggagaaa tccatgacgg taaatagcgg tttggtcagc gtgaccatcc
     7141 atgaaatcag cgacagcagc cccagacgca taatcagcgc cagtgataat cccagcaaac
     7201 gcgctttatc gcgttgtttt ggcggcagtt tgtcagcaag aatggcaatg aagaccaggt
     7261 tatcgatacc cagcacaatt tcgagaacaa caagcgtgag tagccccgcc caaattgagg
     7321 ggtccattaa gaattccatg acaagctcct gcttaaggaa tagctattcg acgccagaaa
     7381 taatgcaggc gtaacgacaa aatgcaaacg aaaggtgcgg catagagtgc cagaaaggca
     7441 ggcgttaaaa ggcctgatgc tgaaatgacg tcggtgacga tccatactgc gggctactgc
     7501 cctatactcc atggttgtta aacgggagtt aaacatatca gagacgcctc tgatttggca
     7561 aagatttacc ttcctttgca aacgaatgtg acaaggatat tttacctttc gaaatttctg
     7621 ctaatcgaaa gttaaattac ggatcttcat cacataaaat aattttttcg atatctaaaa
     7681 taaatcgcga aacgcagggg tttttggttg tagcccttat ctgaatcgat tcgattgtgg
     7741 acgacgattc aaaaatacat ctggcacgtt gaggtgttaa cgataataaa ggaggtagca
     7801 agtgaccatt gctattgtta taggcacaca tggttgggct gcagagcagt tgcttaaaac
     7861 ggcagaaatg ctgttaggcg agcaggaaaa cgtcggctgg atcgatttcg ttccaggtga
     7921 aaatgccgaa acgctgattg aaaagtacaa cgctcagttg gcaaaactcg acaccactaa
     7981 aggcgtgctg tttctcgttg atacatgggg aggcagcccg ttcaatgctg ccagccgcat
     8041 tgtcgtcgac aaagagcatt atgaagtcat tgcaggcgtt aacattccaa tgctcgtgga
     8101 aacgttaatg gcccgtgatg atgacccaag ctttgatgaa ctggtggcgc tggcagtaga
     8161 aacaggtcgt gaaggcgtga aagcactgaa agccaaaccg gttgaaaaag ccgcgccagc
     8221 acccgctgcc gcagcaccaa aagcgactcc aactccggca aaaccaatgg gaccaaacga
     8281 ctacatggtt attggccttg cgcgtatcga cgaccgtctg atacacggtc aggtcgccac
     8341 ccgctggacc aaagaaacca atgtctcccg tattattgtt gttagtgatg aagtggctgc
     8401 ggataccgtt cgtaagacac tgctcaccca ggttgcacct ccgggcgtaa cagcacacgt
     8461 agttgatgtt gccaaaatga ttcgcgtcta caacaacccg aaatatgctg gcgaacgcgt
     8521 aatgctgtta tttaccaacc caacagatgt agagcgtctc gttgaaggcg gcgtgaaaat
     8581 cacctctgtt aacgtcggtg gtatggcatt ccgtcagggt aaaacccagg tgaataacgc
     8641 ggtttcggtt gatgaaaaag atatcgaggc gttcaagaaa ctgaatgcgc gcggtattga
     8701 gctggaagtc cgtaaggttt ccaccgatcc gaaactgaaa atgatggatc tgatcagcaa
     8761 aatcgataag taacgtattg tgttgattat cactcagttt tcacacttaa gtcttacgta
     8821 aacaggagaa gtacaatgga gattaccact cttcaaattg tgctggtatt tatcgtagcc
     8881 tgtatcgcag gtatgggatc aatcctcgat gaatttcagt ttcaccgtcc gctaatcgcg
     8941 tgtaccctgg tgggtatcgt tcttggcgat atgaaaaccg gtattattat cggtggtacg
     9001 ctggaaatga tcgcgctggg ctggatgaac atcggtgctg cagttgcgcc tgacgccgct
     9061 ctggcttcca tcatttctac tattctggtt atcgcaggtc atcagagcat tggtgcaggt
     9121 atcgcactgg caatccctct ggccgctgcg ggccaggtac tgaccatcat cgttcgtact
     9181 attaccgttg ccttccagca cgctgcggat aaggctgctg ataacggcaa cctgacagcg
     9241 atttcctgga tccacgtttc ttctctgttc ctgcaagcaa tgcgtgtggc tattccggcc
     9301 gtcatcgttg cgctgtctgt tggtaccagc gaagtccaga acatgctgaa tgcgattccg
     9361 gaagtggtga ccaatggtct gaatatcgcc ggtggcatga tcgtggtggt tggttatgcg
     9421 atggttatca acatgatgcg tgctggctac ctgatgccgt tcttctacct cggcttcgta
     9481 accgcagcat tcaccaactt taacctggtt gctctgggtg tgattggtac tgttatggca
     9541 gtgctctaca tccaacttag cccgaaatac aaccgcgtag ccggtgcgcc tgctcaggca
     9601 gctggtaaca acgatctcga taacgaactg gactaacagg tgagcgaaat ggttgataca
     9661 actcaaacta ccaccgagaa aaaactcact caaagtgata ttcgtggcgt cttcctgcgt
     9721 tctaacctct tccagggttc atggaacttc gaacgtatgc aggcactggg tttctgcttc
     9781 tctatggtac cggcaattcg tcgcctctac cctgagaaca acgaagctcg taaacaagct
     9841 attcgccgtc acctggagtt ctttaacacc cagccgttcg tggctgcgcc gattctcggc
     9901 gtaaccctgg cgctggaaga acagcgtgct aatggcgcag agatcgatga cggtgctatc
     9961 aacggtatca aagtcggttt gatggggccg ctggctggtg taggcgaccc gatcttctgg
    10021 ggaaccgtac gtccggtatt tgcagcactg ggggccggta tcgcaatgag cggcagcctg
    10081 ttaggtccgc tgctgttctt catcttgttt aacctggtgc gtctggcaac ccgttactac
    10141 ggcgtagcgt atggttactc caaaggtatc gatatcgtta aagatatggg tggtggcttc
    10201 ctgcaaaaac tgacggaagg ggcgtctatc ctcggcctgt ttgtcatggg ggcattagtt
    10261 aacaagtgga cgcatgtcaa catcccgctg gttgtctctc gcattactga ccagacgggc
    10321 aaagaacacg ttaccactgt ccagactatt cttgaccagt taatgccagg cctggtacca
    10381 ctgctgctga cctttgcttg tatgtggctg ttgcgcaaaa aagttaaccc gctgtggatc
    10441 atcgttggct tcttcgtcat cggtatcgct ggttacgctt gcggcctgct gggactgtaa
    10501 gactgttgta cactaccggg gccttttggc cccgtttttt tatctggagg attaatgaca
    10561 atcacggacc tggtactgat tcttttcatc gccgcactcc tggccttcgc gatctacgat
    10621 cagttcatca tgccccgccg taacggcccc accctgctgg caattccttt gctccggcgt
    10681 ggtcgcatcg atagcgttat cttcgtcgga ttgattgtca ttcttatcta taacaacgtc
    10741 acgaatcatg gcgcgctaat aacaacatgg ttattaagtg cactggctct gatgggtttt
    10801 tatatattct ggatccgcgt tccgaagatc atctttaaac aaaaaggttt tttcttcgcc
    10861 aatgtctgga ttgaatatag ccgaatcaaa gcgatgaact tgtcggaaga tggcgtgttg
    10921 gtgatgcaat tagaacagcg ccggctgtta atccgcgttc gaaatatcga cgatctggaa
    10981 aaaatttata agcttctcgt ttcaactcaa taagttatga atttagccaa agctatgttt
    11041 agtgtatttt taataatcag acatagctta ggctatatta cctcttccct tatttgttat
    11101 ttattttaac gtttcattga tatataaatc taaatgaaaa tcgttatcaa taaagcaatg
    11161 aaataatata ttccaacagt tgttttatat tctcaaaata tgttaaggtt gcgccctcat
    11221 ttggggagta gccgatttcc agattccgga aatgtacgtg tcaacatact cgttgcaaaa
    11281 cgtggcacgt acggactgaa cactttcagt caggcgagac catatgcaca tcaatcgcta
    11341 tgcctgcatg aacgcgaaaa cccatcgcgg cttgttgttc atgtatgcat gtttgctggg
    11401 ggcgatgatg tgttttatgg ataccccggt caggacgttg tcatgaatat cactgctact
    11461 gttcttcttg cgtttggtat gtcgatggat gcatttgctg catcaatcgg taaaggtgcc
    11521 accctccata aaccgaaatt ttctgaagca ttgcgaaccg gccttatttt tggtgccgtc
    11581 gaaaccctga caccgctgat cggctgggga atgggcatgt tagccagccg gtttgtcctt
    11641 gaatggaatc actggattgc gtttgtgctg ctgatattcc tcggcgggcg aatgattatt
    11701 gagggttttc gtggcgcaga tgatgaagat aaagagccgc gccgtcggca cggtttctgg
    11761 ctactggtaa ccaccgcgat tgccaccagc ctggatgcca tggctgtggg tgttggtctc
    11821 gccttcctgc aggtcaacat tatcgcgacc gcattggcca ttggttgtgc aaccttgatt
    11881 atgtcgacat tagggatgat ggttggtcgc tttatcggct caattattgg gaaaaaagcg
    11941 gaaattctcg gcgggctggt gctgatcggc atcggcgtcc agatcctctg gacgcacttc
    12001 cacggttaat aagaacgctg ccagaggtga atattaaagt ccgtctggca gtcgaacact
    12061 tcttttgctg ccagtgtttg ccagacttct ggcttcgcac gccaggcaaa cggcgtcatc
    12121 tgcaataatg cgacggcttc atcaccgcga agacgcatcg gataacacaa ctcatcactc
    12181 tgctgtaatg taaaaccttc cagttgttct gcatgtggtg catgaagatg tacttcattg
    12241 taaatcagcc ccttcagctc catcaaatgt cgcggtcccg gcgtggcagt aatgacccag
    12301 ccgccaggct tcactactcg cgctaattct tctgctttac acggcgcgta aatacgtatt
    12361 atggcgtcca tactagtatc ggaaaacggc aaacggtggc tggaagcaac acaaaaagtg
    12421 acctgcggat agcgtttcgc cgccgctttt atcgctacct tcgagacatc caggccaaac
    12481 gtggtgattt cgggcagcgc atcggcaaat gcgtgtgtgt aatacccttc accacagcca
    12541 atatccagca ccgctgtggc cttttcatca agccgttccc gcagttgggc gacaattgca
    12601 tcacgcagcg gctgataatg tccggcatct aagaatgcgc ggcgtgcttg catcatttcc
    12661 gcgctgtcgc ccggatcacg agaccgttta tgctgaacgg gcagcagatt gacataccct
    12721 tctttcgcca tatcaaactg atgtcgctgg ggacagatat agctgttttt ttcacgcgaa
    12781 agaggctgat ggcaaagtgg acaggaaaac gacatgacaa ctccggcagg atactaaagg
    12841 ccgcaagtgt aacgcgaatc acgcggcggg caaatatccg aacgccagga gcgaagataa
    12901 agcaaagtaa aaagcctcgc attcgacgag gctttatagc gagattgaag cgtattcaca
    12961 cttcagatca gtggattcga tcagatagct gttacgttaa cagctgccgg acctttctga
    13021 ccgtcctgaa tttcgaactc aacgttctga ccttcagcca gagttttgaa accattaccc
    13081 tggatagcag agaagtgtac gaacacgtct ttgctgccat cagccggagt aatgaagcca
    13141 aaacctttag actcgttgaa ccacttaacc tgacctttaa tctttgccat ttgaaaaatt
    13201 ccttagattg ttttcttcgc ccgcaggcat aacatagata aaactgacac attactgcat
    13261 gaggcaccaa tataaggctc ggcagagaag cggtattcaa cgtcaacgtg tttactcagg
    13321 acttctttac tgaaaatgcc acacataaac agaactgtac ctcgtttaac ccgaaatctg
    13381 ttatcacata caacgttaat tatggcaagc catttttaaa catgtctcga tcagacgcac
    13441 aaatctcgat acgtcttcca ctttttttgc acacttatgc aacggaatac gcgccgatat
    13501 atcattgtgc ttaaccttgc cagttcaggc agatacttaa actgacgtat tttctaacat
    13561 agttcaataa gttcagttgt tccaggccag tgggagaagt tattacatag tgcgtgcaat
    13621 atcacatttt ctgctatgca atgaataaaa agttatatca ctttttctca taaaacagtc
    13681 agttaacggc caccaattac cccaaagaga agtcaatccc caatggtatt gtaaatttaa
    13741 aataagaaaa actgatgaat gcgcaaaaaa atcaagagag aaacgtttcg ctaataaata
    13801 ataccacgct taatcacaaa tccgatgtaa acatcctaat aaatta
//
LOCUS       DAZGQL010000124        13802 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.124, whole genome shotgun sequence.
ACCESSION   DAZGQL010000124 DAZGQL010000000
VERSION     DAZGQL010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13802
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.124"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..99)
                     /locus_tag="ACHKIR_004394"
     CDS             complement(<1..99)
                     /locus_tag="ACHKIR_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFO9992043.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLT"
     gene            1440..2930
                     /locus_tag="ACHKIR_004395"
     CDS             1440..2930
                     /locus_tag="ACHKIR_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfjI family protein"
                     /protein_id="HFO9992044.1"
                     /translation="MPYFQYPDEFPLSSLPPLIRDAVIEAQQITQAPLGLVAASALGA
                     VSLVCQNLIDVCRLNTLRGPVSLFLLTLAESGERKTAVDKLLMEPLYQQEMLLYSRHK
                     NELTTWKNKEELLKAQKKALLSKLNKELRKGADESETLRQLEALQKNRGEKPVRYKFI
                     FNDATTAAIKDQLCGQWRSVGIMSDEAGIIFDGYTLSELPFINKMWDGSVLSVDRKNE
                     PEQMIENARMTLSLMVQPGLFDRYMERKGSVARDSGFLARCLISKPATTQGKRFINGA
                     VTPGESLTAFHERLMELARGSIEKSSKDERYCLHFSPEAQKIFIDHYNVLEQDLSPSG
                     PLSQFRGHVSKKTENIARIAALLQYFSQGEGKISADIMTSAVVISSWYTDEYKKLFAL
                     PDESELQLQDARELLDWLIEECRGECPPRVRKNYILQCGPGRFRNRKKLNVLLNILAS
                     QLRLSVVQEGKTTYVLLSEINNITLPELNACYSQKLIRWHEQSFGL"
     gene            complement(3052..3624)
                     /locus_tag="ACHKIR_004396"
     CDS             complement(3052..3624)
                     /locus_tag="ACHKIR_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992045.1"
                     /translation="MQKLENRCELLLIQHQKWMTSVTRLIVAHGMGSPHLHGYHRLTL
                     AHFFLPEKGTIISVAPQGLYQVVNPGTPPFIPAIQEGLMTSIQTHEIMLLTHFNLGGV
                     LLSELHRLGENRLANRLNSLLRRFDDRDLYHTLIWLCWYDLMCAHSMQPWTEELKHKS
                     HAELENWAVARKREKRELELMIDEYLLYAC"
     gene            complement(3710..3916)
                     /locus_tag="ACHKIR_004397"
     CDS             complement(3710..3916)
                     /locus_tag="ACHKIR_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO9992046.1"
                     /translation="MNTPVSLMDDQMVDMAFITQLTGLTDKWFYKLIKDGAFPAPIKL
                     GRSSRWLKSEVETWLQARIAQSRP"
     gene            4773..4997
                     /gene="hha"
                     /locus_tag="ACHKIR_004398"
     CDS             4773..4997
                     /gene="hha"
                     /locus_tag="ACHKIR_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HFO9992047.1"
                     /translation="MQHKVTRDEWKLKPEQFRRCSSRETLEKVITRTRYKLTPAELEA
                     FNSAVDHRLAELTMNKLYDRVPASVWKYVT"
     gene            complement(5329..5526)
                     /locus_tag="ACHKIR_004399"
     CDS             complement(5329..5526)
                     /locus_tag="ACHKIR_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001547002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992048.1"
                     /translation="MVIRKKKCRDCGNAITHNTVCCPYCGAVDPFGYYRKTDRLLCLL
                     TLLLVLILVTVSGVSVFVLLQ"
     gene            5725..6294
                     /locus_tag="ACHKIR_004400"
     CDS             5725..6294
                     /locus_tag="ACHKIR_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HFO9992049.1"
                     /translation="MTYKYNPFWQQRIRETVRHALDVHPRLTALRVDLRLPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHSSTLHYAWAREFGELKGKKHYHLLLLVN
                     RDTWCRAGDYRAPGSLAGMIKQAWCSALGVDAGRYDTLAHFPDKPAVWLERDDETGFQ
                     QVLERSGYLAKEHTKARGTGERNFGCSRS"
     gene            6555..6956
                     /locus_tag="ACHKIR_004401"
     CDS             6555..6956
                     /locus_tag="ACHKIR_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFO9992050.1"
                     /translation="MYAKSFLALDGNGRLTGARTAQTAPYDRYTCHLCGSALRYHPQY
                     DTERPWFEHTDDGLTKHGHECPYVRPERREVRLIKRLQQFVPDALPVVRKASWHCRQC
                     HHDYYGEQYCTNCQTGGFSIPRTTQEEICEF"
     gene            6944..7378
                     /locus_tag="ACHKIR_004402"
     CDS             6944..7378
                     /locus_tag="ACHKIR_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFO9992051.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKHNRQDVLCCVSCGCPLTLQR
                     GNDGQPPWFEHDQMTVAEKILLRCTWLDPAEKEARRLHLQGMTVPDYTVKVRKWFCVM
                     CDEDYEGEKCCPRCGTGVYSRAWGRQEVPSEDARADNPLQRL"
     gene            8153..8379
                     /locus_tag="ACHKIR_004403"
                     /pseudo
     CDS             8153..8379
                     /locus_tag="ACHKIR_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013364090.1"
                     /note="with IlvB catalyzes the formation of 2-acetolactate
                     from pyruvate, the small subunit is required for full
                     activity and valine sensitivity; E.coli produces 3
                     isoenzymes of acetolactate synthase which differ in
                     specificity to substrates, valine sensitivity and affinity
                     for cofactors; also known as acetolactate synthase small
                     subunit; frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
     gene            complement(8381..9163)
                     /locus_tag="ACHKIR_004404"
     CDS             complement(8381..9163)
                     /locus_tag="ACHKIR_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HFO9992052.1"
                     /translation="METKQKERIRRLIEILKKTDRIHLKDAARMLEVSVMTIRRDLHQ
                     EDEPLPLTLLGGYIVMVHKPAPSMPVIQDVPRNHRDDLPIAILAAGMVNENDLIFFDN
                     GQEIPLVISMIPDAITFTGICYSHRVFVALNEKPNVTAILCGGTYRARSDAFYDASNS
                     SPLDSLNPRKIFISASGVHDHFGVSWFNPEDLATKRKAMARGLRKILLARHALFDEVA
                     SASLAPLSAFDVLISERPLPADYVTHCRNASVKIITPDSEDE"
     gene            9466..10386
                     /gene="rbsK"
                     /locus_tag="ACHKIR_004405"
     CDS             9466..10386
                     /gene="rbsK"
                     /locus_tag="ACHKIR_004405"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFO9992053.1"
                     /translation="MDIAVIGSNMVDLITYTNQMPKEGETLEAPAFKIGCGGKGANQA
                     VAAAKLNSKVLMLTKVGDDIFADNTIRNLESWGINTTYVEKVPCTSSGVAPIFVNANS
                     SNSILIIKGANKFLSPEDIDRAAEDLKKCKLIVLQLEVQLETVYHAIEFGKKNGIEVL
                     LNPAPALRELDMSYACKCDFFIPNETELEILTGMSVDTYDHIRLAARSLVDKGLNNII
                     VTMSEKGALWMTRDQEVHVPAFKVNAVDTSGAGDAFIGCFSHYYVQSGDVEAALKKAA
                     LFAAFSVTGKGTQSSYPSIEQFNEFLTLNE"
     gene            10414..11730
                     /gene="fucP"
                     /locus_tag="ACHKIR_004406"
     CDS             10414..11730
                     /gene="fucP"
                     /locus_tag="ACHKIR_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0015535 - fucose:proton symporter activity
                     [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HFO9992054.1"
                     /translation="MNDKNIIQMPDGYLNKTPLFQFILLSCLFPLWGCAAALNDILIT
                     QFKSVFSLSNFASALVQSAFYGGYFLIAIPASLVIKKTSYKVAILIGLTLYIGGCTLF
                     FPASHMATYTMFLAAIFAIAIGLSFLETAANTYSSMIGPKAYATLRLNISQTFYPIGA
                     ASGILLGKYLVFSEGESLEKQMSGMNAGQIHNFKVLMLENTLEPYKYMIMILVVVMVL
                     FLLTRFPTCKVAQTSHHKRPSAMDTLRYLARNPRFRRGIVAQFLYVGMQVAVWSFTIR
                     LALELGDINERDASNFMVYSFACFFIGKFIANILMTRFNPEKVLILYSVIGALFLAYV
                     ALAPSFSAVYVAVLVSVLFGPCWATIYAGTLDTVDNEHTEMAGAVIVMAIVGAAVVPA
                     IQGYIADMFHSLQLSFLVSMLCFVYVGVYFWRESKVRTALAEVTAS"
     gene            11742..12755
                     /locus_tag="ACHKIR_004407"
     CDS             11742..12755
                     /locus_tag="ACHKIR_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFO9992055.1"
                     /translation="MSTRINLWRALFGEKPRILLENSDFTVTSFRYDSGVEGLKIANS
                     RGHLIILPWMGQMIWDAQFDGHGLTMCNMFRQPKPATEVIETYGCFAFHSGLLANGCP
                     SAEDTHLLHGEMACAAMDEAWMELEGDMLRLTGRYEYVKGFGHHYLAQPTVVLHKSST
                     LFDIKMAVTNLASVDMPLQYMCHMNYAYIPNATFSQNIPDEILRLRESVPSHVNPTTQ
                     WLAFNQRIMQGEASLSTLNQPEFYDPEIVFFADKLDAYTDQPEFRMIAPDGTTFVTRF
                     SSAELNYVTRWILYNGEQQVAAFALPATCRPEGYLAAQRNGTLIQVAPQQTRTFTVTT
                     GIE"
     gene            13177..13461
                     /locus_tag="ACHKIR_004408"
     CDS             13177..13461
                     /locus_tag="ACHKIR_004408"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO9992056.1"
                     /translation="MEQKTLSAEPRRSFSNEFKLQMVKLALQPRGSVARIAREHDIND
                     NLLFKWLRLSLIKGRISRRLPVTNSSGIGVELLPVEMTLDERPYPVFYAR"
     gene            complement(13677..>13802)
                     /locus_tag="ACHKIR_004409"
     CDS             complement(13677..>13802)
                     /locus_tag="ACHKIR_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011815933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFO9992057.1"
                     /translation="LLITFLPVFLLPVLGDIGFANTTFSDADFGALGILLGIIVR"
BASE COUNT         3602 a         3008 c         3452 g         3740 t
ORIGIN      
        1 ggtgagttta cgcagatgtt ccaggatgtg acgggaaagt gagtaaggca gggaggaaag
       61 cggtttttca attgcctcaa tggcatcaga cggattcata catgtattcc cggtgaaaaa
      121 agacaaaacc ccgcgcaccg gaaaggtggc gggggatcca cggagataat atctgtctga
      181 aattgttttg aatcgatggg attgttgcgt attaacatga aggagatcag gacttatccg
      241 ttactgggtg atctccttcc atgaaaatac gcggttattg ttaaacagca ggctgattac
      301 tccgtaaagg agaaaaatat tcatcttgca gcctgattat caggtgtgtt tatgtttact
      361 gaggtgagta tttttcagta tattgtcggg gcagtcagtg ggagatatta aatttacgtt
      421 tagagttatc ttgcttctgc tgtgtatttt tataagagtg cgcacgtaat gatatctgtt
      481 gataccatgt tgttaagtgt ttcccttccg ggtcatgcgt acttgcaaaa tggcatctga
      541 atcgatcacg gtttggccaa accgtgatcg aatagagagg aatacgcacg taatgattaa
      601 atgcttttgt tacaccattc ttccggcatg aatatttatc cagttccctt ttttatttgt
      661 gtagtgatac tgtcggaata gcgttcagta atgtgaagtg tcctgttaaa aatctttaat
      721 aaatgtgtca ccctggtcag gaaatatctg ctgagatatt attagttggt ggtgttttta
      781 ctgagtaata gctggctttt aattcctgaa ctgaaatgct gtcgctcgtc agggtttaat
      841 taaaactgga tgatgatgtg aaaatgaagg ctggtttttg tgtttgtcga ggaagtgttg
      901 tttttatcca ttctcatcat tggctggctg cgattataca gaagtatttg aagaaaaaat
      961 ggtgcatttt atctgtgccg gcagggagga ggattttcct gatatgtcag tattaaatgg
     1021 tttttgttat ttataaaaat gtcatcctgt ccatagaata ttgtactgag tactcagcat
     1081 tcagcgcagg aactgtcatc ttctttattt tattcttctt caggagggca gttttctgaa
     1141 agcgttactc cggggaaact gtttatgttt attcccggca ggaaacccgt acctgcaata
     1201 ccctggatga tgttttttgc tggtacgttg ttgtgtgaaa attaatcaac atgtgtgctc
     1261 cggagagcgc ccgtagacgc acagattcat tctgacattc tttagcgaca aaataccggg
     1321 tggttattat ttaacctgaa aatcgacaga aatttttatc ctgtattgat tctgctgcta
     1381 tttttgctat tagtagcaat agttgcggta ctttatgttc tgtgtgttgt gaggtcatta
     1441 tgccatactt tcagtatcct gatgagtttc ccctgagctc gctgcctcca ctcattcgtg
     1501 atgccgttat tgaagctcag caaatcacgc aggctcccct ggggcttgtg gcagcatctg
     1561 ccctgggcgc tgtttcactt gtctgccaga atctgattga tgtctgcaga ctgaatacac
     1621 tgcgggggcc ggtttctctt ttcttactga cgcttgctga gtccggagaa cgaaaaaccg
     1681 cagtggacaa attattaatg gagcccctgt accagcagga gatgctgtta tattccagac
     1741 acaaaaatga actgacgaca tggaaaaata aggaagagct tttaaaggcg cagaaaaaag
     1801 cattgttgtc aaaactgaat aaagaactcc gcaaaggcgc ggatgagtca gaaacgctca
     1861 gacaactgga agcgcttcag aaaaacagag gggaaaaacc tgtaagatat aaattcattt
     1921 ttaatgatgc cacgacagca gcaattaaag atcagctttg tggtcagtgg cgttcagtgg
     1981 gtatcatgtc tgatgaagcc ggtataatat ttgatgggta taccctgtct gagctgcctt
     2041 ttattaacaa aatgtgggat ggctccgtat tgtcggtgga cagaaagaat gaacccgaac
     2101 agatgattga aaatgccaga atgacattat ccctcatggt acaacccggt ctttttgacc
     2161 gatatatgga gcgaaaaggt tctgttgcca gggacagtgg ttttcttgcc cgttgtctaa
     2221 tcagtaaacc tgccaccacg caggggaaac gctttattaa cggagcagtg accccgggag
     2281 aatcattaac ggcatttcat gaacgattaa tggaactggc cagaggcagt attgaaaaat
     2341 ccagtaagga tgaacgttat tgtctgcatt tttctccaga agcgcaaaaa atatttattg
     2401 atcactataa tgttctggag caggatttgt ctccttccgg acccttaagc cagtttcggg
     2461 ggcatgtttc aaaaaaaaca gaaaacattg cgagaattgc tgcgttattg cagtatttca
     2521 gtcagggtga agggaaaatc tctgccgata tcatgacgtc tgctgtcgta ataagttcgt
     2581 ggtacacaga tgaatataaa aaattatttg cgttacctga tgaatctgaa ctgcagctgc
     2641 aggacgcccg ggagctgctc gactggctca ttgaggagtg ccgcggagaa tgcccgcccc
     2701 gtgtcaggaa aaattatatt ctccagtgtg gcccgggccg gttcaggaac aggaaaaagc
     2761 tgaacgtcct gctgaacatt cttgcatctc aactcaggct ctctgtcgtg caggaaggaa
     2821 aaaccaccta tgtcctgctt tccgaaataa acaatatcac gctgcctgaa ctgaatgcgt
     2881 gctacagcca gaaactgata agatggcatg agcaatcctt tggcttataa tgttccctgt
     2941 tgctgagacg gattattcag tggtggtatc gcagtatgcc cgtcaccccc cgcttctgca
     3001 tgagggtgac ggaggaaaag taagtgtgcg gaggcccggg agcgttatga atcaacaggc
     3061 gtataacagg tactcgtcaa tcatcagttc cagctcgcgc ttttcccgtt tgcgcgccac
     3121 agcccagttt tcaagctctg catgtgattt atgctttaac tcttcagtcc agggctgcat
     3181 ggagtgggca cacatcagat cataccagca cagccatatc agcgtgtggt agagatcacg
     3241 atcgtcgaaa cgccggagca ggctgttcag cctgtttgcc agccggtttt cacccagacg
     3301 atgtagctca ctgagtaaaa cgccgcccag attaaaatgt gtaagcagca tgatttcatg
     3361 ggtctgaatg ctggtcatta acccttcctg aatggcagga atgaacggtg gtgttcccgg
     3421 gttgacgacc tgatagagtc cctgtggtgc cactgagatg atagtgcctt tttccggcag
     3481 aaaaaagtgg gccagggtca ggcgatgata accatgaaga tgcggagacc ccattccatg
     3541 agcgacgata agcctcgtca cgcttgtcat ccatttctga tgctggatga gcagcagctc
     3601 acaccggttt tcaagttttt gcataatatt ctctccttta gcgcgcagca aaatgcctcc
     3661 gggggccgga tcatgccttt gctgcggttg aacggataac ggtcaggaat tacggacggg
     3721 actgtgcaat acgcgcctgc agccaggttt ccacttcact tttcagccat cgggagctac
     3781 ggcccagttt gatgggggca ggaaaggcac catccttgat gagcttgtaa aaccatttat
     3841 cggtcaggcc ggtcagttga gtgataaatg ccatgtcgac catctggtca tccatcagtg
     3901 aaactggggt attcatgaga atgttccttc ggatgttgtt aatctcaacc ggcgttggtg
     3961 atgaaaacaa cgcaccacct tcaccggcat aacaaccggg gagaaacgta aaaaataatc
     4021 gggaatgtga ccgtcaggag ataaacgggg ggattgcggt ggctgagccg taccggtatg
     4081 tcgggtgatt gttgcgaaac gatgcttccg cacgcgcacg ggaggacttt cgtgagacaa
     4141 ggggaaaact ggcgttctga taattgcaga gttgtttttg aaggcggttg tggtgcatca
     4201 gcctgtgacc tgtgggcagg cgacaggtat cactggtggg ataagactgt ctgattggta
     4261 ttataaatat ataagagagc tcaaacacat gagttcatat atgtgaaata aatctgtgag
     4321 tgtgatcagc agaccagtct acccagtagc aacagattgc gttgtactca cagggttatc
     4381 cctgataact ggcgtataac ctgcgtacat aaaacgtacc tgcaatgcac ctgaacagag
     4441 tgcattaagc atccgcctga gcggtaaacg tatccgatct ggtgagtgac agtctgagag
     4501 cattctcagt aatcgccccc ttgtatcctg gaaattggtt aagagcgatt agatttggca
     4561 aggtgtatgc atccagcagc ttaacacctc agctaactgg ctggttacct tcaggttgtc
     4621 gcaatggcat cggacatccg gtgtcggata actaaacgta gcgttaaggc gatattgctg
     4681 agagaccggt taagtggtgg cacgggagta atgagtttgg ggagggtacc agaatgccag
     4741 tcaggtgcgg tatgctggca gtatcacgtt gattgcagca taaagtaacg agagacgaat
     4801 ggaagttaaa accagagcag ttccgtcgtt gttcatcccg ggagacgctg gaaaaagtga
     4861 ttacccgcac acgttataaa cttacccctg cggagctgga agcctttaac tctgcggtcg
     4921 atcaccggct ggcagaactg acaatgaaca aactttacga tcgcgtgccg gcttccgtct
     4981 ggaaatatgt cacctgaatc ttgaatccgc gctacgatga ctctggtgtt atctgtgcac
     5041 cggaagatcg ggaacattaa gcagtataac ggcaacaaag aattcgaaat caaaagggaa
     5101 tggtgtacag aggggcaggc gttcaggcat taccggcctg tatcttctga aggggactcg
     5161 tatttgtcgg ggctgagtat tccgctcgat tcgatcacgg attggcagaa acctgactgt
     5221 gattgattca ggtaccattt tgcagggatc cggtgcgtta ctgtctggca caacaaacgc
     5281 agcgggtcgc tctgccccgc cgattcctga taattcctcc cctgtaacct actgcaacag
     5341 tacaaataca ctgacgcctg aaacggtaac cagaatcagc accagcagta acgtcagtaa
     5401 acagagcaga cgatcggtct tccggtaata accgaagggg tcgacagcgc cacaatacgg
     5461 gcaacacact gtattatgag taattgcgtt tccacagtcc ctgcacttct ttttgcgtat
     5521 cacgatcgct ccctctccac gccttatccg taacctgttt tttacattaa aaaaacaacg
     5581 ccgggaattc aatcgtcagt ccggagacgg ccgttcgggt tatcacagag tgcctgagac
     5641 agtgtcctgc cggaggtcac ctcaggggct ctgcgcattt tttacgacgt ggctattccg
     5701 tatgaaccat acggagattt aaccatgacc tacaaataca accccttctg gcagcaacgt
     5761 attcgtgaga cggtgcggca cgcactggat gttcatcccc gcctgacggc acttcgggtt
     5821 gatctgcgtc tcccggatgt gccggcagca acggacgcgg ctgtgatatc ccgcttcatc
     5881 aatgccctga aagcccgaat cgacgcttat cagaaacgta agcatcggga aggtaaacgc
     5941 gtgcattcct cgaccctgca ttacgcctgg gcccgggagt tcggggagct taaaggcaaa
     6001 aaacactatc acctgctgct gctggtcaac cgggatacct ggtgtcgtgc cggcgattac
     6061 cgcgctccgg ggtcactggc cgggatgatt aaacaggcct ggtgcagtgc cctgggagtg
     6121 gatgccggac gttatgacac gctggcacat tttcctgaca agccagcggt gtggctggaa
     6181 cgcgatgatg aaaccggctt tcagcaggtg ctggaacgtt ctggctatct ggcgaaggag
     6241 cataccaaag ctcgcggcac cggtgagcgc aattttggct gtagccggag ttgaacacga
     6301 caggcgcacc aatacccggc aaaccaaaag tatgggttgt tgtctggcgg caacgatgta
     6361 tccactcgcc cactgactgt tttttacgtc agtgggccat tgtgtgacaa ccacagtgct
     6421 gtaacagcaa ccagcctgct ttacccaaac tccagtccag attttaactg aaccgccatt
     6481 cacaccctga ccacgctgcc cgtaccggga cggctgttgc ctgcgtgtcg tctggcggct
     6541 gaggaacata tactatgtac gcaaaatcct ttctcgctct tgatggcaac ggacgtctga
     6601 cgggggcccg taccgcacag actgcgcctt acgaccgcta cacctgccac ctttgcggca
     6661 gtgcactcag gtaccacccg caatacgaca ctgaacgtcc ctggtttgaa cacactgacg
     6721 acgggctgac taagcacggt catgagtgcc cttatgtcag accggaacgc agggaggttc
     6781 gcttgattaa gcgtctgcag caattcgtac cggatgcctt acccgtggtg cgtaaagcca
     6841 gctggcactg cagacaatgt caccacgatt attatgggga gcagtactgc acaaactgcc
     6901 agaccggagg attcagtatt ccccggacaa ctcaggagga aatatgcgaa ttctgaactg
     6961 ttatatggcg aatgacagca aaggccattt tgttacggcg aaagaagctg cgaagcacaa
     7021 ccgacaggac gttttgtgct gtgtgtcctg tggatgcccg ttaacacttc agcggggcaa
     7081 tgacggacaa ccaccgtggt ttgaacatga ccagatgact gtcgctgaaa aaatcctgct
     7141 gcgatgtacc tggcttgacc cggcagagaa agaggcccgt cgtttgcatc tgcagggcat
     7201 gacggttccg gattatacgg tgaaggtgag aaagtggttt tgtgtgatgt gtgacgaaga
     7261 ttatgagggg gaaaagtgct gcccacgctg cggtactggg gtatacagca gggcgtgggg
     7321 gcggcaggag gtgccgtcgg aagatgccag ggctgataat ccgttacaga ggctgtaatg
     7381 gttgcctccg gagcagtttg cggtaatgcc gttcccggga atggattgcc agtgcgggga
     7441 ctgtggagct taattccggt gttggtggcc tttccgttga ttttgagcca acagccctcc
     7501 tgtgactgac aggctgcctc gtcattccat tcgtatctcc agtaacaggg ggttgtattg
     7561 tattttaatg tgccggttca ggcgtgcgtt tcccggcgcg tctgcaccgg cttaaccaaa
     7621 ttcaacaggg ataaaatagt gtaaataacc gaagtattac ttgcagaatc gggggaagca
     7681 tccggggttt tattcaacag gcatgggagg ctccggctct gacagacatt gtactgtgga
     7741 atgttgttac aggttggggc ggcaaccgtg ccaggagacc atgagccgtt cacggcatta
     7801 aaggccacac ttcggtcgtt tacattccct gtggtggcag gcatcttccg ttaaggcgtc
     7861 agagcgttac caacgttatg ttgtcatggc tcacctttca tagtcgatgc ttatctggtg
     7921 actgaaacta gccgcagata aagagtgtga agttgatacc atggagtgga gtgacgtgca
     7981 ggcaggagat tcgctctatg cagggttgtg agatgaattc aaaccggctg tcagcgccag
     8041 taatatttga agattcgtcc ggttgttatc cagtgtgcat taaaaaccca gacattatgg
     8101 acaatacaac ggcttccata acagggagga gcctgtttat tatccgggcg taatgactaa
     8161 cctttgtggg ctttttcccg tcgtgctttt aacgttgaag gcattttctg cctgccgatt
     8221 caggacagcg acaaaagcca tatcagacaa ttggtcaatg atgaccagcg tctggagtag
     8281 atgataagcc aaattgataa actggaatat gtgatgaaag tgcaacgtaa tcagtccaat
     8341 ccgacgatgt ttaacaagag tacggtgttt tttcagtaag tcattcgtct tctgaatcag
     8401 gtgtaatgat ctttacagaa gcattccggc agtgcgtaac ataatctgcc ggtaacggac
     8461 gctcgctaat cagaacatca aatgcagaga gcggtgcgag gctggcagag gctacttcat
     8521 cgaacaaggc gtggcgggcg agcaaaattt tccttagtcc acgggccatc gctttacgct
     8581 tagtggcaag atcttcggga ttaaaccagc tgacgccaaa gtgatcatgt acaccgctgg
     8641 cggaaataaa tatttttcgc ggattgagag agtctaatgg cgaagagtta ctggcatcgt
     8701 aaaaagcatc acttctggca cgatacgtac caccacaaag tattgctgtc acattaggtt
     8761 tttcattcaa cgcaacaaag acacgatgtg agtaacagat gccagtgaag gtgattgcat
     8821 ccgggatcat gcttataacg agcggtatct cctggccatt atcaaagaag atcagatcat
     8881 tttcattaac cattccggcg gccagaattg caataggtaa gtcatcacga tgatttctcg
     8941 gaacgtcctg gattactggc atggatggtg cgggtttatg caccattaca atatagccac
     9001 ccagtagggt cagtggcaga ggttcatctt cctgatggag atcgcgacga atagtcatta
     9061 cagaaacttc cagcattcgt gccgcgtctt tcaaatggat tctgtcggtt ttcttaagta
     9121 tttcaatcaa acgtcggata cgctcttttt gcttcgtttc catcaactct ctccgtaaaa
     9181 tgttataaaa ataacattat ggcattagta aacattgttt tagttaccat actatacgac
     9241 cgaccctttg aaatgaaaag ggtatagact gctgaatttt cggtgttaat cctgttaggt
     9301 tgcttatgat tagatagtgt tggcctgagt gataagactg gaagtctatt aaataaacat
     9361 cgggttgatg tgctaatcgc gatctcagtc acaaatgatg caaaagtaac atgctaatgt
     9421 tatttaaata ccattgttat gcgaaagttt ataagaggta gtgaaatgga cattgcagtt
     9481 attggttcca atatggtgga tctcattact tacactaatc agatgcctaa agaaggagaa
     9541 actctggaag cacctgcctt caaaatcgga tgtggtggga aaggtgctaa tcaagcggtg
     9601 gcggcggcta aattgaattc aaaagtcctg atgctgacta aagtaggtga tgatattttt
     9661 gcggataata ctattcgtaa tctcgaatca tggggaatta ataccactta tgttgagaaa
     9721 gtcccctgta ccagcagtgg tgttgcaccg atctttgtta atgccaactc gagcaatagt
     9781 atattaataa ttaagggagc gaataaattt ctttccccgg aagatatcga tcgagctgca
     9841 gaagatctga aaaaatgcaa attgatcgtt ttacaactgg aagttcaact ggaaacagtt
     9901 taccacgcga ttgagtttgg taagaaaaat ggtattgaag tgttattaaa tccggctccg
     9961 gcattgcgtg agttagatat gtcttatgcc tgcaaatgtg atttctttat cccgaatgaa
    10021 acagaactgg aaatattaac cggtatgtcg gttgatactt atgatcatat tcgtttggcc
    10081 gcacgttcgt tagttgataa agggctgaac aatattattg ttaccatgag cgaaaaaggc
    10141 gcattatgga tgacccgcga tcaggaagtc catgttcctg cattcaaagt aaatgctgtt
    10201 gatactagtg gcgctggtga cgcatttatt ggttgctttt cccattatta tgttcaaagc
    10261 ggtgatgttg aagccgcttt gaaaaaagcc gctctctttg ccgctttcag cgtcacaggg
    10321 aaaggtacgc aatcatctta tccgagtatt gaacaattta atgaattttt aaccttaaac
    10381 gaataatcat cctgcacagt taaaggtagc actatgaacg ataaaaacat cattcagatg
    10441 cctgatgggt atctgaataa gactcccttg ttccagttta ttttgttatc gtgtttattc
    10501 ccgctatggg gatgcgcagc tgcattaaat gacatattaa tcactcaatt caaaagtgta
    10561 ttttcgctaa gtaactttgc ttcagcattg gtgcagagcg cattttatgg aggttatttt
    10621 ttaattgcaa taccagcatc ccttgtgatt aaaaagacca gttataaggt ggcaatttta
    10681 attggcctga cgctgtatat tggtggttgt acactttttt tccctgcctc gcatatggca
    10741 acctacacta tgttccttgc agctattttt gcgattgcga ttggcctgag tttccttgaa
    10801 actgcagcga acacatacag ttcaatgatc ggtccaaaag cctatgccac attgcggctc
    10861 aatatcagcc agacctttta tccgattggt gctgcgtctg gtattttatt gggcaaatat
    10921 ctggttttct ccgaggggga aagccttgaa aagcagatgt caggaatgaa cgccgggcaa
    10981 attcacaact ttaaggtgct gatgttagag aacacccttg aaccgtataa gtatatgatt
    11041 atgattctgg ttgtggtcat ggtgttgttc cttttaaccc gcttcccaac ctgtaaagtg
    11101 gcacagacaa gtcaccataa acgcccgtca gcaatggata cgctgcgcta tctggctaga
    11161 aatccgcgct ttcgtcgcgg tattgtggcg cagttccttt atgtgggtat gcaggtcgca
    11221 gtatggtcat ttaccatccg tctggcgctg gagttgggcg atatcaacga acgtgatgct
    11281 tcaaacttca tggtctatag ctttgcctgc ttctttattg gcaagtttat agccaatatc
    11341 cttatgacgc gttttaatcc ggaaaaagta ttgattctct attccgttat aggggcgctg
    11401 ttcctggcat atgttgcact ggctccaagt ttcagcgcgg tctatgtcgc cgtcctggta
    11461 agtgttttgt ttggcccttg ctgggcgacg atctatgcag gaacactgga tacagttgat
    11521 aacgaacata cagaaatggc aggagcagtc attgttatgg ctattgtcgg tgccgcggtg
    11581 gttccggcaa ttcagggata tattgcagat atgttccact cactacagct ttcattcctg
    11641 gtttctatgc tctgctttgt ctatgtcgga gtctacttct ggcgtgaaag caaagtacgt
    11701 accgcactgg ctgaagtcac agcgtcctga ggagaaccgt tatgagtacg cgtattaatt
    11761 tgtggcgggc gctttttggc gagaaacctc gtatcttgct ggaaaatagt gattttactg
    11821 tcacgtcttt tcgatatgac agcggtgtcg aggggcttaa aattgcaaac agtcgtggac
    11881 atttgattat tcttccctgg atggggcaga tgatttggga tgcgcaattt gatggacacg
    11941 gcctcaccat gtgcaatatg tttcgccagc ctaaaccggc gacagaggtg atagagacct
    12001 atggctgttt tgcttttcat tcaggtttgc tggctaatgg ctgcccatct gcggaagata
    12061 ctcatctgct acacggtgaa atggcctgtg ccgcgatgga tgaagcatgg atggaattag
    12121 agggagatat gttacgcctt accgggcgtt acgagtatgt aaaggggttc gggcatcatt
    12181 atctggctca gccaacagtg gtccttcaca aatcctctac actgttcgat attaagatgg
    12241 ccgtaaccaa tctggcttct gtagatatgc cactacaata tatgtgccac atgaattatg
    12301 catatatacc aaatgctacg ttcagtcaga atataccgga cgaaatatta aggttgcgtg
    12361 aatcagtacc ttcgcatgtt aatcccacca ctcagtggct ggcgtttaat caacggatta
    12421 tgcagggaga agcctctctg tcgacgctga atcaaccgga gttttatgat ccagaaattg
    12481 tcttctttgc agacaaactg gacgcttaca cagatcagcc agagtttcgc atgatcgctc
    12541 ccgatggcac tacgtttgtt actcgtttct ccagtgccga actaaattat gtgacacgct
    12601 ggatccttta taacggagag caacaggtag ccgcctttgc attacctgca acctgtcggc
    12661 ctgaggggta tcttgccgcg caacgtaatg gcacgctgat tcaggttgca ccgcagcaaa
    12721 cgcgcacttt tacagtcacc acaggaattg aataaccact caaggcctga acagcggcgc
    12781 gcttatcgtt aaggtaagcg cgttttttta cccgccagga caagactatg atagcgttgc
    12841 ccttatagac gatcacatta tcgtccactc caaatttgcg caactactgt agagtggtgt
    12901 actccacagc ttctttgagg agcttaacct ccattgtttt cttaccgagc aagcactgca
    12961 actcttttat ttatttgtag tggtcaacaa aaactggtca caatttcagt ttttttccag
    13021 tatctatttt ccgattcgtt cggcggtaat ccaccgttat attcatgagg ccggagtgca
    13081 ctataaaagt gtatgcgtaa accgactgct gtatgtagcc attaagccag tattggtaac
    13141 ataggtaccc accttttcaa ctagtggaca cctgttatgg aacaaaaaac attatcagct
    13201 gaaccccgca ggtcattttc aaatgagttt aaacttcaaa tggttaaact ggctttacaa
    13261 ccaaggggtt ctgttgcccg tattgcccgg gagcatgata tcaatgataa tcttctgttc
    13321 aaatggctca ggctctcgct gatcaaaggg cgtatatcac ggcgtcttcc ggtaacgaac
    13381 tcttctggca ttggcgttga attattacct gtggagatga cgctggatga gcggccttac
    13441 cccgtttttt acgcgaggta attatgaaga tcaatgacta gccgtcgaac cgtgtgcatg
    13501 aactgctgca ctgaaacttc tcagctgtaa aataatcctt acgctacgta cttcccggag
    13561 cgcttacgat taatcggatg cagtcctgac tggaagaaag aaaaatggag aaaataagag
    13621 gaattaatat cctccacgtt cctgaaatcc tggaggatat taatgtcatc aaaatattag
    13681 cgaacgataa tccctaacaa aatacccagt gcgccaaagt cagcatcact gaaggtggtg
    13741 ttagcaaaac caatatcacc cagcaccggc aataagaata ctggcaggaa ggtaataagc
    13801 aa
//
LOCUS       DAZGQL010000125        13036 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.125, whole genome shotgun sequence.
ACCESSION   DAZGQL010000125 DAZGQL010000000
VERSION     DAZGQL010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13036
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.125"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            298..1356
                     /gene="citC"
                     /locus_tag="ACHKIR_004410"
     CDS             298..1356
                     /gene="citC"
                     /locus_tag="ACHKIR_004410"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008771 - [citrate (pro-3S)-lyase] ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006101 - citrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HFO9992058.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFCHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1371..1667
                     /gene="citD"
                     /locus_tag="ACHKIR_004411"
     CDS             1371..1667
                     /gene="citD"
                     /locus_tag="ACHKIR_004411"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HFO9992059.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            1664..2572
                     /gene="citE"
                     /locus_tag="ACHKIR_004412"
     CDS             1664..2572
                     /gene="citE"
                     /locus_tag="ACHKIR_004412"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HFO9992060.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2583..4115
                     /gene="citF"
                     /locus_tag="ACHKIR_004413"
     CDS             2583..4115
                     /gene="citF"
                     /locus_tag="ACHKIR_004413"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HFO9992061.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYSGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVYLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGAAVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            4119..4670
                     /gene="citX"
                     /locus_tag="ACHKIR_004414"
     CDS             4119..4670
                     /gene="citX"
                     /locus_tag="ACHKIR_004414"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050519 - holo-citrate lyase synthase
                     activity [Evidence IEA];
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HFO9992062.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHVWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKLGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            4645..5523
                     /gene="citG"
                     /locus_tag="ACHKIR_004415"
     CDS             4645..5523
                     /gene="citG"
                     /locus_tag="ACHKIR_004415"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HFO9992063.1"
                     /translation="MSMPATSTKNTKLATSLIDEYALLDWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLNGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSRLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMATNGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            5574..7037
                     /gene="citT"
                     /locus_tag="ACHKIR_004416"
     CDS             5574..7037
                     /gene="citT"
                     /locus_tag="ACHKIR_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HFO9992064.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFANTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            7151..7957
                     /gene="rna"
                     /locus_tag="ACHKIR_004417"
     CDS             7151..7957
                     /gene="rna"
                     /locus_tag="ACHKIR_004417"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HFO9992065.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETATKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISVKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            8187..8597
                     /gene="rnk"
                     /locus_tag="ACHKIR_004418"
     CDS             8187..8597
                     /gene="rnk"
                     /locus_tag="ACHKIR_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HFO9992066.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            8740..8829
                     /gene="yldA"
                     /locus_tag="ACHKIR_004419"
     CDS             8740..8829
                     /gene="yldA"
                     /locus_tag="ACHKIR_004419"
                     /inference="COORDINATES: protein motif:HMM:NF041476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YldA"
                     /protein_id="HFO9992067.1"
                     /translation="MSEALGITAFYIFIAAIIVAAVLYLEQRW"
     gene            8954..9382
                     /gene="uspG"
                     /locus_tag="ACHKIR_004420"
     CDS             8954..9382
                     /gene="uspG"
                     /locus_tag="ACHKIR_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HFO9992068.1"
                     /translation="MYKTIIMPVDVFEMELSDKAIRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAK
                     ELDADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(9503..11068)
                     /gene="ahpF"
                     /locus_tag="ACHKIR_004421"
     CDS             complement(9503..11068)
                     /gene="ahpF"
                     /locus_tag="ACHKIR_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFO9992069.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(11239..11802)
                     /gene="ahpC"
                     /locus_tag="ACHKIR_004422"
     CDS             complement(11239..11802)
                     /gene="ahpC"
                     /locus_tag="ACHKIR_004422"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFO9992070.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            12174..12920
                     /gene="dsbG"
                     /locus_tag="ACHKIR_004423"
     CDS             12174..12920
                     /gene="dsbG"
                     /locus_tag="ACHKIR_004423"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HFO9992071.1"
                     /translation="MLKKILLLALLPAIAFAEELPAPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGGKLKLSVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKMLNIIMGNK"
BASE COUNT         3115 a         3414 c         3270 g         3237 t
ORIGIN      
        1 tcgtggcaaa ttttattcat taaatcaatt aaatcactta aacctgcggg tcatttcgta
       61 attacattaa ttaaaaaact taaaaccatt aaataaataa aaaaccacta actctatgtg
      121 aaataaatca aaatttcacg ctgcaatact ccttaggatg tatagcgaaa ggagaaaaag
      181 atatacctcg atcgcccccc tttctcccaa gtgaaaataa aaggttatca gtttgcaaca
      241 tcgaacaaca tttgttgcaa atcgataaca acatgcacct tcaggatact atttattatg
      301 ttcggcaatg atattttcac ccgtgtaaaa cgttcagaaa ataaaaaaat ggcggaaatc
      361 gcccaattcc tgcatgaaaa tgatttgagc gttgacacca cagtcgaagt atttattacc
      421 gtaacccgcg atgaaaagct tatcgcgtgc ggtggaattg ccggaaatat tattaaatgc
      481 gttgctatca gtgaatccgt tcgcggtgaa ggactggcgc tgacattagc caccgagcta
      541 attaacctcg cctatgagcg gcacagcacg catctattta tttataccaa aaccgaatac
      601 gaggcgctgt tccgccagtg cggtttttcc acgctgacca gcgtacccgg cgtgatggtg
      661 ctgatggaaa acagcgccac gcgactgaaa cgctatgccg aatcgctgaa aaaattttgt
      721 catccaggga acaagattgg ctgcattgtg atgaacgcca atccctttac gaatggtcac
      781 cgttatctga ttcaacaggc tgcggcacag tgcgactggt tgcatctgtt tttagtcaaa
      841 gaagattctt cacgcttccc ctatgaagac cgactggatc tggtgttaaa aggcaccgcc
      901 gatattccac gcctgactgt gcatcgcggc tccgaataca tcatctcccg cgctacgttc
      961 ccttgctact tcattaaaga acagagcgtc attaaccatt gttacaccga aattgatctg
     1021 aagattttcc gtcagtacct cgctcccgcg ctgggtgtaa ctcaccgctt tgtcggtact
     1081 gaaccctttt gtcgcgttac tgcccagtac aaccaggata tgcgctactg gctggaaaca
     1141 ccgactatct ccgcaccgcc catcgaactg gttgaaattg agcggctgcg ttaccaggag
     1201 atgccgatat ccgcttcccg ggtacgtcaa ctgctggcga aaaacgatct cacggctatc
     1261 gcgccgctgg tccctgcagt cacgctgcat tatttgcaga acctgcttga gcactcccgc
     1321 caggacgcgg cagctcgtca aaagaccccc gcatgagaaa caggtgaaaa atgaaaataa
     1381 accagcccgc cgttgcaggc acccttgagt ctggggatgt gatgatacgc atcgccccac
     1441 tcgatacgca ggatatcgac ctgcaaatca acagcagcgt tgagaaacag tttggcgatg
     1501 caattcgcac caccattctg gacgttctcg cccgctacaa cgtgcgcggc gtacagctga
     1561 atgtcgatga caaaggcgca ctggactgca ttttacgtgc acgactggaa gccctgctgg
     1621 cacgcgccag cggtatcccg gctctgccat gggaggattg ccaatgattt ccgcttcgct
     1681 gcaacaacgt aaaactcgca cccgccgcag tatgttgttt gtgcctggtg ctaatgccgc
     1741 gatggtcagc aactccttta tctacccggc tgatgccctg atgtttgacc tcgaagactc
     1801 cgtagcattg cgtgaaaaag acaccgcccg ccgcatggtt taccacgcgc tgcaacatcc
     1861 gctttatcgc gatattgaaa ccattgtgcg tgtaaacgcg ctggattccg aatggggtgt
     1921 taacgacctg gaagctgtcg ttcgcggtgg tgcggacgtt gtgcgtctgc cgaaaaccga
     1981 taccgctcag gatgttctgg atatcgaaaa agagatcctg cgtatcgaaa aagcctgtgg
     2041 tcgtgaaccc ggcagtaccg gcctgctggc ggcgattgaa tctccgctgg gcattacccg
     2101 cgcggtagaa atcgctcacg cttccgagcg tttgatcggt atcgccctcg gtgcagaaga
     2161 ctatgtgcgc aacctgcgta cagaacgctc cccggaagga actgaactgc tgttcgctcg
     2221 ctgctccatt ttgcaggccg cgcgctctgc gggtattcag gcgttcgata ccgtctattc
     2281 cgacgctaac aacgaagccg gattcctgca agaagccgcc cacatcaaac agctgggctt
     2341 tgacggcaaa tcgctgatca acccgcgtca gattgatctg ctgcacaacc tctacgcacc
     2401 gacccagaaa gaagtggatc acgcccgccg cgtcgtagaa gccgctgaag ccgccgctcg
     2461 cgaaggcctc ggcgtggttt ccctgaacgg caagatggtg gacggtccgg ttatcgatcg
     2521 cgcccgtctg gtgctctccc gtgcagaact ttccggcatc cgcgaagaat aaggcaatca
     2581 aaatgacgca gaaaattgaa caatctcaac gacaagaacg ggtagcggcc tggaatcgtc
     2641 gcgctgaatg cgatcttgcc gctttccaga actcgccaaa gcaaacctac caggctgaaa
     2701 aagcgcgcga tcgcaaactg tgcgccaacc tggaagaagc gattcgtcgc tctggtttac
     2761 aggacggcat gacggtttcc ttccatcacg ctttccgtgg cggtgacctg accgtcaata
     2821 tggtgatgga cgtcatcgcg aagatgggct ttaaaaacct gactctggcc tccagctccc
     2881 tgagtgattg ccatgcgccg ctggtagaac acattcgcca gggcgtggtt acccgcattt
     2941 atacctctgg cctgcgcggt ccactggcgg aagagatctc ccgtggtctg ctggcggaac
     3001 cggtgcaaat ccactctcac ggcggacgtg tgcatctggt acagagcggc gaactgaata
     3061 tcgatgtggc tttcctcggc gtcccgtcct gtgatgaatt cggtaatgcc aacggctact
     3121 ccggtaaagc ctgctgcggc tccctcggct atgcaatggt tgatgccgac aacgcaaaac
     3181 aggtcgtgat gcttaccgaa gaactgctgc cttatccgca taatccggca agcattgagc
     3241 aagatcaggt tgatctgatc gtcaaagttg atcgcgttgg cgatgctgca aaaatcggcg
     3301 ctggtgctac ccgtatgacc actaacccgc gcgaactgct tattgcccgt agcgctgcgg
     3361 atgtgattgt taactctggc tacttcaaag aaggtttctc catgcagacc ggcaccggcg
     3421 gcgcttcgct ggcggtaacc cgtttcctgg aagacaaaat gcgtagccgc gatattcgcg
     3481 ccgacttcgc cctcggcggt attaccgcaa cgatggttta tctgcacgaa aaaggtttga
     3541 tccgcaaact gctggatgtg cagagctttg acagccatgc tgcgcaatcg ctggcccgta
     3601 accccaatca catcgaaatc agcgccaacc agtacgctaa ctgggggtcg aaaggcgcag
     3661 cggttgatcg tctcgacgtg gtggtactga gcgcgctgga aattgacacc cagttcaacg
     3721 ttaacgtgct gaccggctct gacggcgtac tgcgtggtgc ttccggtggt cactgcgata
     3781 ccgcgattgc ttctgcgctt tccatcatcg tcgcgccgct ggtacgcggt cgtattccga
     3841 cgctggtgga taacgtgctg acctgcatca ccccgggctc cagtgtcgat attctggtca
     3901 cagaccacgg catcgctgtt aacccggcac gtccggagct ggcagaacgt ctgcaggaag
     3961 cgggcattaa agtggtttcc attgagtggc tgcgtgaacg tgcgcgtctg ctgaccggtg
     4021 aaccacagcc gattgaattc accgaccgcg tcgttgccgt tgtgcgttac cgcgatggct
     4081 cggtgatcga tgttgtgcat caggtgaagg aataagccat gcacctgctt cctgaactcg
     4141 ccagccacca tgcggtgtca attcccgagc tgctcgtcag ccgggatgaa aggcaagcac
     4201 ggcaacacgt ctggctcaag cgccatcctg ttccactggt ctcctttacc gtggttgcgc
     4261 ctgggccgat taaagacagc gaggtcacac gccgaatttt taatcatggc gtgacagcct
     4321 tgcgtgcctt agccgcaaaa ctgggctggc aaattcagga gcaggctgca ctggtttccg
     4381 ccagcgggcc ggagggcatg ttgagcattg ccgccccggc tcgcgacctc aagctcgcca
     4441 ccattgagct tgaacatagt catcctctcg ggcggttatg ggatatcgat gtcctgacac
     4501 ccgaaggcga aattctctcc cgccgcgact attcactgcc gcctcgccgc tgcctgttgt
     4561 gcgagcaaag cgcagctgtt tgcgcgcgtg gaaaaaccca tcaactgacc gatttactca
     4621 accgcatgga ggcgctgctg aacgatgtcg atgcctgcaa cgtcaactaa aaacacaaag
     4681 cttgcgacgt cattaatcga tgagtacgcc ctgctggact ggcgcgccat gctgactgaa
     4741 gtcaatctgt caccgaaacc aggcctcgtg gatcgcatta actgcggtgc gcacaaagat
     4801 atggcgctgg aagatttcca ccgcagcgcg ctggcgattc agggctggtt accccgtttc
     4861 attgaatttg gtgcctgtag tgcagaaatg gcaccggaag cggtactcaa cggattacgc
     4921 ccaattggta tggcttgcga aggtgatatg ttccgcgcca ctgcgggcgt aaacacgcat
     4981 aaaggaagca ttttttcttt agggctactg tgtgcggcaa ttggtcgttt gcttcagctc
     5041 aaccagccgg taacgccaac aaccgtttgt tctacggcgg caagtttctg ccgtggcctg
     5101 accgatcgcg aactgcgtac caataattca cgactgacgg caggtcaacg gttgtaccag
     5161 cagcttggcc ttaccggcgc acgcggtgaa gccgaagcgg gttatccact ggtgatcaac
     5221 cacgccttgc cgcattacct cactctgctg gatcaggggt tagatcctga gctggcattg
     5281 ctcgatacct tgctcctact gatggctacc aacggcgata ccaacgttgc atcgcgcggt
     5341 ggcgaggggg gcctgcgctg gctacagcgc gaggcgcaaa cattattgca aaaagggggc
     5401 attcgaaccc ccgccgatct cgattatctc cggcagttcg acagggagtg tatcgaacga
     5461 aatctcagtc caggcggcag tgccgacctg ctgatcctta cctggttttt agcacagatt
     5521 taattattta agcacttgat aaatttggaa atattaattt tcggagaacc cgtatgtctt
     5581 tagcaaaaga taatatatgg aaattattgg cgcccctggt ggtgatgggt gtcatgtttc
     5641 ttatccctgt acctgacggt atgccgccgc aggcgtggca ttactttgca gtgtttgtgg
     5701 caatgattgt cggcatgatc ctcgagccaa ttccggcaac ggcgatcagt tttattgcgg
     5761 ttactatttg cgttattggc agtaattacc tgctctttga tgccaaagaa ttagctgacc
     5821 cagcgtttaa tgcgcaaaaa caggcgctga aatggggtct ggctggtttt tccagcacca
     5881 cggtatggct ggtatttggc gcatttattt ttgcattagg gtatgaagtt tccgggttag
     5941 gccgtcgtat tgcccttttc ctggtgaaat tcatgggcaa acgcacgctg acgttgggtt
     6001 atgcgattgt cattatcgac attctgctgg caccgtttac accgtccaac accgcgcgta
     6061 ccgggggtac ggtttttccg gtcattaaaa acctgccgcc gttgtttaaa tcattcccga
     6121 acgatccgtc cgcgcgtcgt attggcggct atttgatgtg gatgatggtc attagtacca
     6181 gtctgagttc gtccatgttt gtcaccggtg cggcaccaaa cgtgctgggt ctggagttcg
     6241 tcagcaaaat tgccggtatc cagattagct ggttgcagtg gttcctctgc ttcctgccgg
     6301 ttggggttat cttgcttatc attgcgccgt ggctttccta cgtgctgtac aaaccggaaa
     6361 tcacacacag tgaagaagtg gcaacctggg cgggtgatga actaaaaacc atgggtgcgc
     6421 tgacacgcag agagtggacg ctgattggcc ttgtattgct cagcttaggt ttgtgggtat
     6481 ttggcagtga agtcattaat gctactgcgg ttggtctgct ggcagtttcg ctaatgctgg
     6541 ccctgcacgt tgtgccgtgg aaagacatta cccgctataa cagcgcatgg aacacgctgg
     6601 tcaacctggc aactctggtt gtgatggcta acggcctgac tcgttctggt tttattgact
     6661 ggttcgccaa taccatgagt acgcacctgg aaggattctc accaaacgca acggtgattg
     6721 tactggttct ggtgttctac tttgcacact acctgtttgc cagcctgtct gcgcacaccg
     6781 caaccatgct gccggttatt ctggccgtcg gtaaaggtat tccgggcgta ccaatggaac
     6841 aactgtgtat cctgctggtg ctatctatcg gtatcatggg ctgtctgacg ccgtatgcaa
     6901 ccggtcctgg ggtgattatt tacggctgtg gctatgtgaa atcaaaagat tactggcgtc
     6961 ttggcgcaat cttcggggtg atttacatct ctatgttgct gttggttggc tggccgattc
     7021 tcgccatgtg gaactaatac gttcgccaaa cgctgaataa atcatcacgc ccgccaggta
     7081 aacccacctg gcgggctttt ttatgattaa atagataata ttcagtgtat gtatgagttc
     7141 cacacccatt atgaaagcat tctggcgtaa cgccgcgttg ctcgcggttt ctctgcttcc
     7201 cttctcttct gccaacgccg tggcgttgca ggcaaaacag tatggcgatt ttgatcgcta
     7261 tgtcctggcc ctctcctggc aaaccggatt ttgccagagt caacacgatc gaaatcgtaa
     7321 cgaacgagat gaatgtcgcc tgcaaaccga aacgaccaac aaagctgatt ttctgaccgt
     7381 acatggtctg tggccaggat tgcctaaatc ggttgctgcc cgtggtgttg atgaacgccg
     7441 ctggatgcgc ttcggttgcg ctactcgccc aatcccgaat ctaccagaag cgcgcgccag
     7501 ccgaatgtgt tcatcgccgg aaaccgggtt atcactggaa acggccacta aactaagtga
     7561 agtcatgcca ggagctggcg gacgttcctg cctggaacgc tacgaatatg ccaaacacgg
     7621 tgcctgcttt ggttttgatc cagacgcata cttcggcacg atggtacgcc tgaatcaaga
     7681 aattaaagaa agcgaagccg gaaaattcct tgcggataac tacggtaaaa cagtgagccg
     7741 ccgtgacttt gatgccgcct ttgccaaaag ctggggaaaa gagaacgtga aagcagttaa
     7801 gctaacgtgc cagggtaacc ctgcgtattt gactgaaatt cagatctcgg tcaaagctga
     7861 cgccatcaac gctccgcttt ctgccaactc attcttgcca caaccacacc caggcaactg
     7921 tggcaaaacc tttgtgattg ataaagcagg ttattaatcc tctgatttac aaaacacgct
     7981 aattcgtctt ccataaacca tgacgattaa gatttataca taatgcgcta cattgaggaa
     8041 ttccctgaat gtgacgcaaa tcacttcaaa gagtgagcaa cagtcagtat catcatgtga
     8101 gttaaaccct cgccgcctga cggtgagggt tttcttttgg gtatgtttat ctgcgacact
     8161 cgcagtaccg acgacatgga gtaaaaatgt ccagaccaac tatcatcatt aacgacctgg
     8221 atgccgaacg catcgatatt ctgctggagc aacccgccta tgctggtttg ccaatcgccg
     8281 acgcgttaaa cgcagagttg gatcgcgccc aaatgtgttc gccagaagag atgccacacg
     8341 acgtggtgac aatgaacagc cgggttaaat tccgcaatct tagcgatggc gaagtgcgtg
     8401 tgcgcacgct ggtgtatccg gcaaaaatga ccgacagcaa tactcagctt tccgttatgg
     8461 ctccggtagg tgccgcactg ctggggctgc gcgttggcga ttccattcac tgggaacttc
     8521 cgggcggcgt tgcaacccac cttgaagtgc tggaactcga ataccagcca gaagctgctg
     8581 gcgactacct gctttaatcc catctgcaca agcgataact cgctttgccc agaggatgca
     8641 ctcgcatcct ctttccgctc aaatatcaac cagatttatc attttgctct taagcgcagc
     8701 attctaatct gaactggcta ttcatctgcg gaacctcata tgagcgaagc tctcggtatc
     8761 actgcatttt atatattcat agcagcaatt attgttgctg cagtgctata ccttgaacaa
     8821 cgctggtagc gcctggtttt tatttatttt ccagctcaat agcaaattca gatcaaatca
     8881 gcctcgcaaa ttatagattc gtaaccaata atgtgctgtt atgtatacat gcaaaaacaa
     8941 ggagagaatg cttatgtaca aaacaatcat catgccagtg gatgttttcg aaatggaatt
     9001 gagcgacaaa gcgattcgcc acgctgaatt cctcgcccag gatgacggag ttattcatct
     9061 acttcacgta ctaccggggt cagccagcct gagcctgcac cgttttgccg ctgatgtacg
     9121 tcgttttgaa gagcatctgc aacatgaagc agaagaacgt ctgcaaacga tggtcagcca
     9181 cttcaccatc gatccttccc gcattaagca acatgtccgt tttggtagtg tgcgggatga
     9241 agttaacgag ttagcgaaag aacttgatgc agatgtagta gtgattggtt cgcgtaaccc
     9301 atcaatttca actcatctgt tgggttctaa cgcctcgagc gtaatccgcc acgccaattt
     9361 gccggtgctg gttgtgcgtt aattgccaga ataagtctcc cgccctgccc ggagccatcc
     9421 ctgcagggct ttttaatgca ataaaaaagc cgccaggttt gaccccggcg gctaagcaat
     9481 tacaggtgaa tcttacttct tcttatgcag ttttggtgcg aatcaggtag tcaaaagcac
     9541 tcagagaggc tttggcacct tcgccagtgg cgatgatgat ctgcttgtac ggaaccgtcg
     9601 tacagtcacc cgctgcgaac acgcctttga cgttggtttc gcatttcgca tcaatgataa
     9661 tctcgcccat gcgattacgt tcgactgcgc cttccagcca gttggtgttc ggcagcagac
     9721 caatctgcac gaaaataccg gccagttcga tgttgtgaat atcgccgctg acacgatctc
     9781 gatattccag acctacgact ttgctaccgt cgcctttcac ttccgtggtt tgcgcattca
     9841 gaataatgtc gacgtttttc aggctgcgca gtttgtcctg cagaacctgg tcggctttca
     9901 tttctggcgc aaattccagc agcgttacgt gctcaacgat acccgccagg tcaattgccg
     9961 cttccacgcc ggagttaccg ccgccgataa ccgctacgcg tttaccttta aacagcgggc
    10021 cgtcgcagtg cgggcagtag gtcacgcctt tggtgcgata ctgatcttcg cccggaacgt
    10081 tcatgttgcg ccattttgca ccggtcgcca cgataatgct gcgtgctttc agtaccgcgc
    10141 cagaagctgt ttcaatctga tgcaggccac cttcaaccgc tgccgggatc agtttgctgg
    10201 cgctctggct gtcgatcaca tcaacgtcgt attcatcaac gtgaactttc agcgctcctg
    10261 ccagtttctg gccttcggtc ttcggtacag aaatgtagtt ttcgatatca acggtatcga
    10321 ggatctgacc accaaaacgt tcgcccatca gaccggtacg gatgccttta cgtgcggagt
    10381 aaattgctgc cgctgcaccc gccgggccgg aaccgacgat taatacgtca taagcatcac
    10441 gcttgttcag ctcttctgcc gcacgttttt ccgcaccagt atcaattttg gcaacgattt
    10501 cagtcaacgt catgcggccc tgaccaaact ctttcccgtt tacgaacact gccggaacgc
    10561 ccatcacgtt gcgatcggtg atttcgttct ggaaggtgcc gccgtcaatt gcagtgtgct
    10621 tgatgcgcgg gttcagtacg ctcatcaggt tcagcgcctg caccacgtcc gggcagttgt
    10681 ggcaagagag cgagtaatag gtttcgaatt caaaatcacc gtcaatatgg cgaatctgct
    10741 ccagcagaga ctgcgcttct ttcgacggat gaccaccggt ccacagcaac gccagtacca
    10801 gcgaggtgaa ctcgtggccc agcggggagc ctgcaaaacg tggcccctgg ttagaacctg
    10861 ggttggtgat caggaaagac ggcttacgca ccggcaagct gttatcttct ttaaaggtga
    10921 ctttgtctga cagttctgcg atttcagcca acagttcctt gatttctgcc gatttagcgc
    10981 tgtcatccag cgtggcaatt aactcaacag gcttggtcaa tttctcaagg taagccttga
    11041 gttgagtttt catatttgtg tcgagcatgt ttatctcctg ggctttaaac atcatcatgc
    11101 aagcggcctc tgcggctacc tggatgcagc ttgcatcatg gtgcggggaa gaaaatcggg
    11161 tgccgcagca cccgaagaat taggtgaatt tccgaagtat ttcgcgccgg agttagtagc
    11221 gcgaaagaca aaggaaattt agattttacc aaccaggtcc agagacggag ccagagttgc
    11281 ttcaccttct ttccatttag ccgggcaaac ttcacctggg tgagaagcta cgtactgtgc
    11341 tgctttgatt ttacgcagca ggtcagacgc gtcacggcca atgccttcag cggtaacttc
    11401 gattgcctgg atgataccct gcgggtcaac aacgaaggtc gcacggtcag ccagaccttc
    11461 atcttcacgc atgttgtcga agttacgggt cagggcgcca gtcgggtcgc cgatcatcgc
    11521 atatttgatt ttagcgatgg tttcagagct gctgtgccat gctttgtggg tgaagtgagt
    11581 atcggtagat actgcgtata cgtctacgcc cagtttctgc aattcttcgt aatggtcagc
    11641 aacgtcaccc agttcggtcg ggcatacgaa agtaaagtca gccgggtaga agaagaagac
    11701 gctccagcgg ccttcggtat ctttttcggt gatttcgatg aattcgccgt ttttgaatgc
    11761 ctggttttta aaaggtttaa ttttggtgtt aatcaaggac atctatactt cctccgtgtt
    11821 ttcgatgaga tgtaaggtaa ccaatttttg ccgattcggc taatgcgttt ccattatcaa
    11881 atcgataagt tttaccttac aaccttcgta agacaacttt gtgaactttg caccgccagc
    11941 ggcgataaaa agtaaaatgg ccccctttcc aggtgaccgt gtaatctgaa ctaccctttg
    12001 gtatcttcag cgcctgcctg caggctgagg tgttgccctt acaaatgcaa caacgacatg
    12061 gattacaaca ccctcataaa caaagggcaa tcacctgatc taagctctta cctatgacag
    12121 tgataggtta tgccttttac tcgacttttg cactgactga aaaggacaaa ttaatgttaa
    12181 aaaagatact tttactggct ctgcttcctg caatcgcctt cgcagaggaa cttcctgccc
    12241 cagtaaaagc gattgaaaaa cagggtatta caatcatcaa aacgttcgat gcccctggag
    12301 gaatgaaagg ttatctcggt aagtatcagg atatgggcgt caccatctac ctgactccag
    12361 atggtaagca cgctatctct ggttacatgt acaacgaaaa aggtgaaaac ctgagtaaca
    12421 cgcttatcga aaaagaaatt tacgcacccg ccggacgtga aatgtggcaa cggatggaac
    12481 aatcccactg gctcctcgac ggtaaaaaag atgcgccggt cattgtctac gtcttcgccg
    12541 atccgttctg cccatattgt aaacaattct ggcagcaggc tcgtccgtgg gtagattctg
    12601 gcaaagtgca attaagaaca ttgttggttg gggttatcaa gccagaaagc ccggcgacag
    12661 cagcggcaat tcttgcctcc aaagatcccg caaaaacctg gcaacaatat gaagcctctg
    12721 gtggcaagct taagctaagc gtgcctgcaa acgtaagcac agagcaaatg aaagtgttaa
    12781 gcgacaacga gaaactcatg gacgatctgg gggcaaatgt cacaccagct atctattaca
    12841 tgagtaagga aaataccctg caacaagccg tcggattacc cgatcagaaa atgcttaata
    12901 tcattatggg gaataaataa aagataattc atggatggtt gaaatatatc cttgcttttc
    12961 aaccatccta aaaacgcatg aattgctcgt aacccacctg acagctatgc tattttagaa
    13021 aatcttaatc caaagt
//
LOCUS       DAZGQL010000126        12741 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.126, whole genome shotgun sequence.
ACCESSION   DAZGQL010000126 DAZGQL010000000
VERSION     DAZGQL010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12741
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.126"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            35..568
                     /locus_tag="ACHKIR_004424"
     CDS             35..568
                     /locus_tag="ACHKIR_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO9992072.1"
                     /translation="MNTKIKYGLSAAVLALIAAGAPAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGAIMVDGKPVIPGMKLSKGKCDRVNAIERDKALAWVEKNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWGIDR"
     gene            866..1333
                     /locus_tag="ACHKIR_004425"
     CDS             866..1333
                     /locus_tag="ACHKIR_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFO9992073.1"
                     /translation="MNRVLCVVIIALLVACGALSLGLNHYRDNAITYKAQRDKKAREL
                     EQANATITDMQVRQRDVAALDAKYSRELADARAENETLRADVAAGRKRLRINATCHGT
                     VREATSTSGVDNATGPRLADTAERDYFILRERLMTMQKQLEGAKEYIRTQCIN"
     gene            1357..1581
                     /locus_tag="ACHKIR_004426"
     CDS             1357..1581
                     /locus_tag="ACHKIR_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992074.1"
                     /translation="MKKLLVTVEPFQGTIPFRILQRGRVLVEGSFSGKCTQLHSRTFQ
                     VNATNEELTVECTMNAAKCRMVSAALQPVC"
     gene            2029..2577
                     /locus_tag="ACHKIR_004427"
     CDS             2029..2577
                     /locus_tag="ACHKIR_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFO9992075.1"
                     /translation="MNVNKKKLAEIFNVDIRTIDRWQTQGLPQISGGGKGVEVIFDSA
                     KAIEWYAQREADIENEKLRKEVEDLRAASESDLQPGTIDYERYRLTKAQADAQELKNA
                     REEGLVLETELFTYIFQRVAQNISGILVRVPQTLQRKYPEISPAHLDAMKTEIAKASD
                     VASEAGENVRRWIDDFRRTEGG"
     gene            2549..4477
                     /locus_tag="ACHKIR_004428"
     CDS             2549..4477
                     /locus_tag="ACHKIR_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFO9992076.1"
                     /translation="MISDELRAANSAGAIATGLLALKIPVPLTTVQWADRHYYLPKES
                     SYTPGRWETLPFQVAIMNSMGNDRIRTVNLIKSARVGYTKMLLGVEAYFIEHKSRNSL
                     LFQPTDSAAEDFMKSHVEPTIRDVSVLLDLAPWFGRKHRDNTLTLKRFSSGVGFWCLG
                     GAAAKNYREKSVDVVCYDELSSFEPDVEKEGSPTLLGDKRIEGSVWPKSIRGSTPKIK
                     GTCQIEKAANESAHFMRFYVPCPHCGEEQYLKFGDESTPFGLKWEKDSPESVFYLCEH
                     HGCVIHQSELDQSNGRWICENTGMWTRDGLTFFSAAGNEIPPPRSITFHIWTAYSPFT
                     TWVQIVYDWLDALKDPNGLKTFVNTTLGETWEEAVGEKLDHQVLMDKVVRYTAAVPAR
                     VVYLTAGIDSQRNRFEMYVWGWAPGEEAFLVDKIIIMGRPDEEETLLRVDAAINKKYR
                     HADGTEMTISRVCWDTGGIDGEIVYQRSKKHGVFRVLPVKGASVYGKPVITMPKTRNQ
                     RGVYLCEVGTDTAKEILYARMKADPTPADEATSYAIRFPDDPEIFSQTEAQQLVAEEL
                     VEKWEKGKMRLLWDNKKRRNEALDCLVYAYAALRVSVQRWQLDLAVLAKSREEETTRP
                     TLKELAAKLSGGVNGYSR"
     gene            4461..4667
                     /locus_tag="ACHKIR_004429"
     CDS             4461..4667
                     /locus_tag="ACHKIR_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family head-tail joining protein"
                     /protein_id="HFO9992077.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTAASLDELN
                     RAINDAESVLGTTRRRRRPLGVRL"
     gene            4664..6256
                     /locus_tag="ACHKIR_004430"
     CDS             4664..6256
                     /locus_tag="ACHKIR_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO9992078.1"
                     /translation="MKRTPVLIDVNGVPLRESLSYHGGGAGFGGQMAEWLPPSQSADA
                     ALLPALRLGNARADDLVRNNGIAANAVALHKDHIVGHMFLISYRPNWRWLGMRETAAK
                     SFVDEVEAAWSEYAEGMFGEIDVEGKRTFTEFIREGVGVHAFNGEIFVQPVWDTETTQ
                     LFRTRFKAVSPKRVDTPGHGIGNRFLRAGVEVDRYGRAVAYHICEDDFPRSGSGRWER
                     IPRELPTGRPAMLHIFEPVEDGQTRGANQFYSVMERLKMLDSLQATQLQSAIVKAMYA
                     ATIESELDTEKAFEYIAGAPQGQQDNPLINILEKFSSWYDTNHVTLGGVKIPHLFPGD
                     DLKLQTAQDSDNGFSALEQALLRYIAAGLGVSYEQLSRDYSKVSYSSARASANESWRY
                     FMGRRKFIAARLATQMFSCWLEEALLRGIIRPPRSRFDFYQARSAWSRAEWIGAGRMA
                     IDGLKEVQESVMRIEAGLSTYEKELALMGEDYQDIFRQQVRESAEREKAGLSRPVWIA
                     QAYQQQIAESRRPEEETRPRET"
     gene            6246..7751
                     /locus_tag="ACHKIR_004431"
     CDS             6246..7751
                     /locus_tag="ACHKIR_004431"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFO9992079.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSVPQ
                     QQVQLDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADTSVRGVLLDIDSPGGQAAGAFDCADMIYRLRQQKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMGHVSYAGHLAQAGVDITLIYAGAHKVDGN
                     QFEALSAEVCQDMQQRIDAAHRMFAEKVAMYTGLSVEAVTGTEAAVFEGQSGIEAGLA
                     DELINASDAISVMAAALNTHDTGGTMPQLTATEAAAQENQRVMGILTCQEAKGREQLA
                     TMLAGQQGMSVEQARAILAAAAPQQPVASAQSEADRIMACEEAKGREQLAATLAAMPE
                     MTVEKARPILAASPQADAGPSLRDQIMALDEAKGAEAQAEKLAAFPGMTVEAARDILS
                     SSPDKAEPVSASTTAMFERIMANHSPAAVQGGVSQTSADGDADVKMLMAMP"
     gene            7788..8135
                     /locus_tag="ACHKIR_004432"
     CDS             7788..8135
                     /locus_tag="ACHKIR_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFO9992080.1"
                     /translation="MVTKTITEQRAEVRIFAGNDPAHTATGSSGISQATPALTPLMLD
                     EASGKLVVWDGQKAGSAAGILVLPLEGTETVLTYYKSGTFATEAIRWPESVDEHKKAN
                     AFVGTALSHAALP"
     gene            8193..9221
                     /locus_tag="ACHKIR_004433"
     CDS             8193..9221
                     /locus_tag="ACHKIR_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFO9992081.1"
                     /translation="MGLFTTRQLLGYTEQKVKFRALFLELFFRRTVNFHTEEVMLDKI
                     TGKTPVAAYVSPVVEGKVLRHRGGETRVLRPGYVKPKHEFNYQQAVERLPGEDPAQLN
                     DPTYRRLRIITDNLKQEEHAIVQVEEMQAVNAVLYGKYTMEGEQFDTVEVDFGRSEGN
                     NIEQADGKKWSEQDRDTFDPTHDIDLYCDQASGLVNIAIMDGTVWRLLNGFKLFREKL
                     DTRRGSNSQLETAVKDLGAVVSFKGYYGDLAIVVAKTSYVAEDGTEKRYLPEGTLVLG
                     NTSAEGIRCYGAIQDAQALSEGVVASSRYPKHWMTVGDPAREFTMTQSAPLMVLPDPD
                     EFVVVQVK"
     gene            9273..9656
                     /gene="gpFI"
                     /locus_tag="ACHKIR_004434"
     CDS             9273..9656
                     /gene="gpFI"
                     /locus_tag="ACHKIR_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFO9992082.1"
                     /translation="MATKEENLNRLRQLAGLLGREADMSGSAADIAQRVSEWEEELAA
                     SREGIMHSDESGSDQNDTDDGEPLNNTDAPDDVKAVRVRKCLHVMGYCPETGRPVELT
                     FRGMRVLVPSSLAMAMIQHGTAEYA"
     gene            9649..10002
                     /locus_tag="ACHKIR_004435"
     CDS             9649..10002
                     /locus_tag="ACHKIR_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFO9992083.1"
                     /translation="MRDVQNAFDAALVGVDSTIVEVMGIRVQFTSGAQRGGEVQGVFD
                     DPESLGFAGGGVRIEGSSPSLFVRTDTVRAVRRGDTLTINGEMFWVDRVSPDDGGSCY
                     LWLNRGQPPAVNRRR"
     gene            10018..10593
                     /locus_tag="ACHKIR_004436"
     CDS             10018..10593
                     /locus_tag="ACHKIR_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9992084.1"
                     /translation="MAIKGLDQAIDNLSRVRKNAIPAASAMTINRVATTTINQSSSQV
                     ARETKVRRKLVKERSRLKRATVRNPNARIIVNRGDLPVIKLGIRMLGRRPDSILKAGQ
                     HRYQRAFIQRLNNGRWHVMQRLPQARYEKGNDDKGRKKRNRLPIQVVKIPMAAPLKQA
                     FDENVDRIRRERLPKELAYALKQQLRIAIKR"
     gene            10590..10985
                     /locus_tag="ACHKIR_004437"
     CDS             10590..10985
                     /locus_tag="ACHKIR_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFO9992085.1"
                     /translation="MKHTDIRAAVLDALEKHDTGATLFDGRPAVFEEADFPAVAVYLT
                     DAEYTGEELDADTWRATLHIEVFLPAQVPDSELDQWMESRIYPAMAAIPALAGMITTM
                     VTQGYEYRRDDDMALWSSADLTYSITYEM"
     gene            10993..11742
                     /locus_tag="ACHKIR_004438"
     CDS             10993..11742
                     /locus_tag="ACHKIR_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9992086.1"
                     /translation="MATPNPLAPVKGSGTTLWVYTGKGDAYANPLSDDEWTRLAKIKD
                     LTPGEMTAESYDDNYLDDEDADWVSTGQGQKSAGDTSFTLAWKPGEKGQRDLIAWFDS
                     SETRAYKIRFPNGTVDVFRGWVSAIGKAVTAKEVITRTVKITNIGRPSLAEDRGEITP
                     VTGITVTPLTGNVAKGQSTTLTVAVQPEGATDKTFRATSANQNFATITVKGNTITVKG
                     VAAGKAQIPVVTGNGEFAAVAEITVTDGAAG"
     gene            11759..12181
                     /locus_tag="ACHKIR_004439"
     CDS             11759..12181
                     /locus_tag="ACHKIR_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFO9992087.1"
                     /translation="MFLKTEQFEYNGVSVTLSELSALQRFDYMKFVSDAEQQGTTKHD
                     VVHINQRYLETASLLVAMSLWHSHSLKGTLASPETEMQQIRREVMLGWPADALNQATN
                     RVLYLSGMLDNRHDADPEQTGKAEATEPVTSKKHSKAS"
     gene            12208..12621
                     /locus_tag="ACHKIR_004440"
     CDS             12208..12621
                     /locus_tag="ACHKIR_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFO9992088.1"
                     /translation="MGRPDWRAMLAGMTSTEYADWRRFYCTHYFQDTQLDAHFSGLMY
                     AVLSLFFGDPDMHPADFSLLAPVCEEEQTEMPDEEDMLMQKAAGVAGGVRFCGVGGGD
                     ISPSADVVDVSEDDVALMMASAGISGGVRYVPASG"
     gene            12602..>12741
                     /locus_tag="ACHKIR_004441"
     CDS             12602..>12741
                     /locus_tag="ACHKIR_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO9992089.1"
                     /translation="MSQPAGDLVIDLSLDAARFDEQMARVRRHFSSLEADARKTASTV
                     EQG"
BASE COUNT         3002 a         3017 c         3891 g         2831 t
ORIGIN      
        1 gggattcgga accgataaat cctgataaat atccatgaac accaaaatca aatacggcct
       61 gtcggctgcc gttctggcgc tgattgccgc aggtgcgcct gcgcctgaaa tcctcgacca
      121 gtttctggat gaaaaagaag gcaaccacac cacggcatac cgtgatggcg cgggtatctg
      181 gaccatctgc cggggtgcca tcatggtgga tggtaagcct gtgattcctg gcatgaagct
      241 gtcgaagggg aaatgcgacc gggttaacgc cattgagcgt gataaggcgc tggcatgggt
      301 ggagaaaaac atcaaagtgc cactgaccga accccagaaa gcggggatcg cgtcattctg
      361 tccttacaac atcggcccag gtaagtgttt tccgtcgacg ttttataaac gaattaatgc
      421 aggcgatcgc aggggagcgt gtgaagcgat tcgctggtgg attaaggacg gtggcagaga
      481 ctgccgtatc cgctcaaata attgctacgg tcaggtatcc cgtcgcgacc aggagagtgc
      541 gctggcatgc tggggtatcg acaggtagta gaatattttg ctgaaaaatg acgttgacca
      601 acgcggacgg ataacacgaa atcctgcgaa ctggcaaaac ctaagtgaat aaaagtaaaa
      661 accccgtttg ttggcagcaa gcggggtttt gtgtttcctg actccggaaa agtcaaagga
      721 gaaagtgtgt ttgattttag caaactgatt cgggagattc gagtgatggc tgaaaaatta
      781 tccacctgga agttcatcct tatctggctg gtgtttgtga ttatggcctc cggttatttc
      841 atcggtcaga tacgctggtg gtgaaatgaa ccgcgttctg tgtgtggtca tcattgcctt
      901 gctggtggcc tgtggtgcgc ttagtctggg gctgaatcat taccgcgata acgccatcac
      961 ctacaaagcg cagcgcgata aaaaagccag agagctggaa caggcgaatg ccaccattac
     1021 tgacatgcag gtgcgccagc gtgatgttgc tgcgctcgat gcaaaatact cgagggaatt
     1081 agccgatgcg agagctgaaa atgaaactct gcgtgctgat gttgccgctg gtcgtaagcg
     1141 cctgcggatc aacgccacct gccacggtac cgtgcgtgaa gccaccagca cctccggcgt
     1201 ggataatgca accggccccc gactggcaga caccgctgaa cgggattatt tcatcctcag
     1261 agaacggttg atgacaatgc agaagcagct ggaaggggcg aaggaatata tccgcactca
     1321 gtgtattaac tagtattttt gttatccgga gaatgcatga agaaattact ggtaaccgta
     1381 gaaccttttc agggaacaat tccgttccgt attttgcagc gtggtcgtgt tcttgttgaa
     1441 ggttcgttca gtggtaaatg tacgcaatta cactcccgga cctttcaggt gaatgccacg
     1501 aatgaagagc taaccgttga gtgtacgatg aatgccgcta aatgccgcat ggtatccgct
     1561 gcattacagc cagtgtgttg agcgacctta ttatccatgc gcggtattgt cgccgtattc
     1621 ccgtattaac agagaccgca gcccgacagg gagactcctc tgcgcgagtg tgcggggata
     1681 atcaaaaacg atacacaccg gggtttaccg cgttaacgga gcgcggcgtt gtcccctcat
     1741 ggtcgctggt ccggtgcgat ggtggaagaa actggatttt gttgcaactg ataaccatta
     1801 tcattttcgc gggtcctttc cggcgatccg ggccgttacg gggcggcgac ctcgcgggtt
     1861 ttcactattt atgaaaattt tccgggaaaa agcatgtcag ttcttcttgc tgttaacata
     1921 ttgtttttaa agaaataatc atcaagaaag aaacgacatc agaacgctga aaaatctgtc
     1981 ttttttggca tttcatgtcg taactgattt ttgataagga attgatgaat gaatgttaat
     2041 aaaaagaagc ttgctgaaat ttttaatgtc gatatacgca ctattgatcg ctggcaaact
     2101 caggggctcc ctcagatatc tggtggagga aaaggtgttg aggtcatctt tgattctgcg
     2161 aaggcgatcg aatggtatgc tcagcgcgaa gctgatattg aaaatgaaaa actccgtaaa
     2221 gaggtcgagg atttgagggc tgccagcgaa tcagaccttc agcccggcac cattgattac
     2281 gaacgttacc gactgacgaa ggcacaggca gatgcacagg agctgaaaaa tgctcgtgag
     2341 gaaggccttg tcctcgagac ggagttattt acctacatct ttcagcgagt ggcacagaat
     2401 atatctggga tccttgtccg tgtccctcag acactgcagc gtaaataccc tgagatatca
     2461 cccgcacatc ttgatgctat gaaaactgaa atcgcgaaag catccgatgt ggcttctgaa
     2521 gccggtgaga atgtgcgcag gtggattgat gatttcagac gaactgaggg cggctaattc
     2581 tgcaggagcg atagcaaccg gccttcttgc gctaaaaatt cctgtccccc tgacgacagt
     2641 tcagtgggca gatcgacact attaccttcc gaaagagtca tcttacaccc cggggcggtg
     2701 ggaaacactg ccatttcagg ttgccatcat gaacagcatg gggaatgacc ggatccgcac
     2761 ggttaatctg attaaatctg cccgtgttgg ttatacaaag atgttgctgg gagtggaggc
     2821 ttattttatt gagcataaat cacgcaacag ccttcttttc cagcctacgg attctgctgc
     2881 tgaagatttt atgaaatctc atgtggaacc cacgatcagg gatgtgtcgg ttttactcga
     2941 tcttgcaccg tggtttgggc gtaaacatcg tgataatacc ctcacgctga aacgcttttc
     3001 atcgggtgtg ggtttctggt gcctgggtgg tgcggcagca aaaaactacc gtgaaaaatc
     3061 cgtggatgtg gtctgctatg acgaactttc ctcgttcgag ccggatgttg aaaaagaggg
     3121 ctcgccaact ctgctggggg ataagcgtat tgagggctcg gtatggccaa aatccattcg
     3181 cggctcgacg cctaaaataa aaggcacctg ccagatcgaa aaagcggcca acgagtcggc
     3241 gcatttcatg cgtttttatg tgccctgccc gcactgtggg gaggagcagt atctgaaatt
     3301 tggcgatgaa tccacgcctt ttgggcttaa atgggagaag gacagtcccg aaagtgtttt
     3361 ctacctctgt gaacatcatg gctgcgtgat ccatcagtct gagcttgacc agagcaacgg
     3421 gcggtggatc tgtgaaaaca cggggatgtg gacccgtgac ggtctgacgt ttttcagcgc
     3481 tgcgggtaat gaaattccgc cgccgcgctc catcacattc catatctgga cggcgtacag
     3541 tccgttcacc acctgggtac agattgtcta tgactggctg gatgcactga aagatcccaa
     3601 cggcctgaaa acttttgtga acaccacgct gggcgagacc tgggaagagg ccgtgggcga
     3661 aaaactcgat caccaggtgc tgatggataa ggttgtgcgt tacacggcgg cggtacctgc
     3721 ccgggtggtt tatctgacgg cgggcattga ctcgcagcgt aaccgttttg agatgtatgt
     3781 ctggggatgg gctccgggag aggaagcctt tctggtggat aaaatcatca ttatggggcg
     3841 tcctgatgag gaagagactc tgttacgtgt ggatgcggcg atcaacaaaa aataccgcca
     3901 tgcagacgga accgaaatga ccatttcccg tgtctgctgg gacaccgggg ggatcgatgg
     3961 cgaaatcgtt tatcagaggt caaaaaaaca cggtgttttc cgggtgctgc cggtaaaagg
     4021 cgcatctgtc tatggcaagc cggtgatcac catgccaaaa acccgcaatc agcggggcgt
     4081 gtatctgtgt gaagtgggga cggacaccgc aaaagaaatt ctctatgccc gtatgaaagc
     4141 cgatcccacg cctgcggatg aagccacgtc gtatgccatc cgttttcctg atgatccgga
     4201 gattttttcg cagacagagg cgcagcaact ggtcgcggaa gagcttgtgg agaagtggga
     4261 aaaaggaaag atgcgtctgc tgtgggataa caaaaagcgg cgtaacgaag cgctggactg
     4321 cctggtgtat gcctacgcgg cattacgtgt gtccgtgcaa cgctggcagc ttgatctggc
     4381 tgtactggca aaatcccggg aagaagagac gacccggcca accctgaaag aactggcagc
     4441 gaagctgtcc ggaggagtga atggttacag tcgctgaact gcaggcgctg cgtcaggcgc
     4501 gccttgattt attaaccggt aaacgggtgg tgtctgtcca gaaagatggt cgcagaattg
     4561 aatatacggc ggcttctctg gatgagctta accgtgcgat caatgatgcg gagtcggtac
     4621 tggggacaac ccgacgtcgc cgtcgtccgc tgggagtgag gttatgaaac gaacgcctgt
     4681 cctgattgat gtgaacggcg ttccgcttcg ggagagcctc agctaccacg ggggcggtgc
     4741 aggatttggt gggcaaatgg cagagtggtt gccaccctcg cagagtgccg atgcggccct
     4801 gctgcccgcg ttgcgtctgg ggaatgcccg tgcagatgat ctggtacgca ataacgggat
     4861 agcggctaat gcggtggccc tgcataagga tcatattgtc gggcatatgt ttctgatcag
     4921 ctaccgtccg aactggcgct ggctggggat gcgggagact gcggcaaaaa gctttgtcga
     4981 tgaggtggag gcggcctggt cggaatacgc cgaagggatg tttggcgaga tcgacgtgga
     5041 aggaaaacgc acgttcacgg aatttatccg tgaaggcgtg ggcgttcatg cgtttaacgg
     5101 cgaaatcttt gtgcagccgg tctgggatac ggaaaccacg cagttattcc gcacgcgttt
     5161 taaagccgtg agtccgaaac gggtggacac gccaggacac ggtatcggga atcgttttct
     5221 gcgggccggg gtggaggtcg atcgatatgg gcgtgccgtt gcgtaccata tctgtgagga
     5281 tgattttcct cgctccggga gtggacgatg ggaacggatc ccgcgtgaac ttcccaccgg
     5341 gcgtccggcc atgctgcata ttttcgagcc ggtggaggac gggcagaccc gtggggccaa
     5401 tcagttttac agcgtcatgg aacggctgaa gatgctcgat tccctgcagg caacacagct
     5461 tcagtcagcc attgttaaag ccatgtatgc agcgacgatt gaaagtgaac ttgataccga
     5521 aaaggccttt gaatatatcg cgggggcacc gcagggtcag caggataatc cgctcattaa
     5581 tattctggag aagttctcca gttggtatga cacgaatcac gtgacgctgg gcggtgtcaa
     5641 aattccgcac cttttccccg gtgatgatct gaaactgcag accgcgcagg attcagacaa
     5701 tggattttct gcgcttgaac aggcgctgct gcggtatatc gccgccggtc ttggcgtttc
     5761 ctacgaacag ttgtcccgtg attactcgaa ggtcagttac tcaagtgccc gcgcctccgc
     5821 caatgagtcg tggcgctatt ttatggggcg gcgaaaattt attgcggccc ggctggccac
     5881 gcagatgttt tcctgctggc tggaagaggc acttcttcgg gggattatcc gtccgccacg
     5941 gtcacgtttt gatttttatc aggcgcgatc agcctggtca cgggcagagt ggattggtgc
     6001 cggaagaatg gccattgacg ggctcaagga ggtccaggaa tcagtgatgc gcattgaggc
     6061 cggactgagc acgtatgaga aagagctggc gctgatgggt gaggattatc aggacatttt
     6121 ccgccagcag gtcagggaat ccgcagagcg ggaaaaagcc ggactctcac gtccggtgtg
     6181 gatagcacag gcgtatcagc agcagatagc ggagagccgc aggccggaag aggagacaag
     6241 accacgtgag acgtaatctt tcacacatta ttgccgcagc attcaatgaa ccgctgcttc
     6301 tggagcccgc ctatgcgcgg gttttctttt gcgcgctcgg gcgcgagatg ggggcagcaa
     6361 gtctttcggt accacaacag caggtgcagc ttgatgctcc cggaatgctg gctgaaacgg
     6421 acgagtacat ggccggaggt aaacgaccgg cccgtgttta ccgggtggtg aacggtattg
     6481 ctgtactgcc ggtgaccggc acgctggtgc accggctggg tggcatgcgg ccattttccg
     6541 gaatgacagg ctatgacggc attgttgcct gtcttcagca ggcaatggcg gacacctctg
     6601 tccggggcgt actgctggat attgacagtc cgggcgggca ggccgccggt gcgtttgact
     6661 gcgctgacat gatttaccgc ctccggcagc agaagccggt ctgggcactg tgtaatgaca
     6721 cggcctgttc tgcggccatg ctgctggcgt cggcctgctc ccgacggctg gttacccaga
     6781 catcccgtat cggctccatt ggcgtgatga tgggccatgt cagctatgcc ggtcatctgg
     6841 cgcaggccgg ggtggatatc acgctgattt atgccggggc gcataaggtg gatggcaatc
     6901 agtttgaagc cttatcggca gaggtgtgtc aggacatgca gcagcgcatt gatgcggcgc
     6961 accggatgtt tgccgaaaaa gtggcgatgt atacggggct gtctgtggaa gctgtcacgg
     7021 ggacagaggc tgccgttttt gaaggtcagt ccggtattga ggccgggctg gcggatgagt
     7081 taatcaatgc gtcggatgcc atcagcgtga tggctgcggc gctgaacaca catgatacag
     7141 gaggcactat gccgcaatta actgcaacgg aagccgccgc gcaggagaac cagcgagtga
     7201 tggggatcct gacgtgtcag gaagcgaaag gacgtgaaca gcttgccacg atgctggcag
     7261 gacaacaggg catgagcgtt gaacaggccc gggcgattct ggctgcggca gcaccacagc
     7321 agccggtggc atccgcgcag agtgaagccg atcgcattat ggcgtgtgaa gaagctaaag
     7381 gtcgtgaaca actggcggca acgctggcgg cgatgccgga gatgacggtg gaaaaagccc
     7441 gcccgatcct ggcggcttca ccgcaggcgg atgccggacc ctcactccgt gatcagatta
     7501 tggctttgga tgaggcaaaa ggggctgagg cgcaggctga aaaactggcg gcgtttcccg
     7561 gaatgacggt ggaggctgcc cgcgacattc tgtcctcatc gccggataaa gcagaaccgg
     7621 tttctgcatc cacaaccgcc atgtttgaac gcatcatggc gaaccattca ccggcagccg
     7681 tgcagggtgg cgtgtcacag acgtcggcag acggtgatgc ggacgtgaaa atgctcatgg
     7741 ccatgccatg aagtcagtgc tgaccatcaa tatgaggttt ttacaatatg gtaacgaaaa
     7801 ccatcactga acaacgtgca gaagtacgta tttttgccgg taatgatccg gctcataccg
     7861 ccacaggcag cagcgggatt tctcaggcaa caccggcact gacgcccctg atgctggatg
     7921 aagccagcgg gaaactggtg gtctgggacg gacagaaagc cggtagtgcg gctggcatac
     7981 tggtactgcc gcttgaaggc acagagacgg tgctgacgta ttacaagtcg ggaacctttg
     8041 cgacggaggc aatccgctgg cctgaaagtg tggatgaaca caaaaaggcc aacgcctttg
     8101 tcggcacagc cctgagtcac gcggcgctgc cgtaacacgt tatcaggcca ccgcgttggc
     8161 ctgactgatt tctgaatgaa aggaactgat ttatgggatt gtttacgacc cgccagttac
     8221 tcggttatac cgaacaaaaa gtgaaatttc gtgcgctgtt tctggaactg tttttccgcc
     8281 gtacggtgaa tttccacacc gaagaggtga tgctggacaa aattaccgga aaaacgccgg
     8341 tggcagccta tgtctccccg gttgttgaag gaaaagtgct gcgtcatcgt ggtggtgaaa
     8401 cccgcgtgtt acgtccgggc tacgtcaagc cgaaacacga atttaattac cagcaggcgg
     8461 tggagcgcct tcccggtgaa gatccggcgc agctgaacga cccgacctac cgtcgtctgc
     8521 gtatcatcac cgataacctc aaacaggaag agcacgccat tgtccaggtg gaagaaatgc
     8581 aggcggtgaa tgccgtgctg tatggcaaat acaccatgga aggggagcag tttgatactg
     8641 tcgaggtgga tttcgggcgc tctgaaggaa ataacattga gcaggctgac ggtaaaaaat
     8701 ggtctgagca ggaccgtgat acgtttgatc cgacgcatga tattgacctc tactgcgatc
     8761 aggccagcgg ccttgtgaat atcgccatta tggacggtac ggtctggcgt ctgctgaatg
     8821 gctttaagct gttccgcgaa aaactggata cccgtcgcgg ctcaaattca caactcgaaa
     8881 cggcagtgaa agatctgggc gcagtggtgt ccttcaaggg gtattacggc gatctggcca
     8941 ttgtggtggc gaaaacgtct tatgtggcag aggacggtac cgaaaaacgt tatctgccgg
     9001 agggcacgct ggtcctggga aatacgtcag cggagggcat ccgttgctat ggtgccattc
     9061 aggatgcgca ggcgttgtcc gaaggtgtgg tggcctcttc ccgttatccg aaacactgga
     9121 tgaccgtggg cgatccggcc cgtgaattca ccatgacgca gtccgctccg ctgatggtgc
     9181 tgccggatcc ggatgagttt gtggtggtac aggtgaaata atccgggagc gggggcgaaa
     9241 tgcccccgtg tcttttttca cagggggctg agatggcaac aaaagaagaa aatctgaatc
     9301 gtcttcgtca actggctggt ctgctggggc gcgaggcgga tatgtcgggg agtgctgcgg
     9361 atattgctca gcgtgtgtct gagtgggaag aggagcttgc tgcttcccgg gagggcatta
     9421 tgcactctga tgagagcggg tctgatcaaa atgacacaga cgatggtgag ccgttgaaca
     9481 acacggatgc tccggatgat gttaaagccg tccgtgtgcg caagtgcctg catgtgatgg
     9541 ggtattgccc ggagacaggc cgtcctgttg agcttacgtt ccgggggatg cgtgttctgg
     9601 tgccatcatc actggcaatg gccatgatac agcacggaac ggctgagtat gcgtgatgtt
     9661 cagaatgcct ttgatgccgc ccttgtgggg gtggacagca cgattgttga agtgatgggg
     9721 atccgtgtgc agtttacctc cggagcacaa cgtggcggtg aagttcaggg ggtttttgac
     9781 gatccggagt cgctggggtt tgccggtggc ggggtccgta ttgaaggaag cagcccgtca
     9841 ttatttgtgc ggacggatac ggtgcgtgcc gtgcggcgtg gtgacacgct gaccattaac
     9901 ggcgagatgt tctgggtgga tcgtgtttct ccggatgacg ggggcagctg ttatctctgg
     9961 ctcaaccgtg ggcaaccacc cgctgttaac cggcgacgat aaacgcaggg tgaaattatg
    10021 gcgataaaag ggcttgatca ggcgattgat aatctgagcc gggttcgtaa aaacgccatt
    10081 ccggcggctt cagcaatgac gattaaccgc gtggccacaa cgacgattaa tcagtcttca
    10141 tcacaggttg cccgggagac aaaggtacgc cggaaactgg ttaaggaacg gtccagactg
    10201 aaacgggcca cggtcagaaa tccgaatgcc agaattatcg ttaaccgcgg tgatctccct
    10261 gttattaagc tggggatcag aatgctgggg cgtcgtccgg acagcatact caaagccggt
    10321 cagcatcgtt atcagcgggc atttatccag cgattaaata atgggcgctg gcatgttatg
    10381 caacgtcttc cccaggccag atatgagaag ggcaatgacg acaagggcag gaaaaagcgt
    10441 aatcgccttc ccattcaggt ggtgaaaatc ccgatggcgg ccccactgaa acaggcgttt
    10501 gatgaaaacg ttgaccgtat ccgtcgtgaa cgcctgccca aagaactggc atatgcgctg
    10561 aaacaacaac tgaggattgc gataaaacga tgaaacatac tgatatccgt gcagccgtac
    10621 tggatgcact ggagaagcat gacaccgggg cgacgctgtt tgatggtcgc cccgctgttt
    10681 ttgaggaggc ggattttccg gcggtcgcgg tttatctgac ggatgcagag tataccggtg
    10741 aagagctgga tgctgatacc tggcgggcca cactgcatat tgaggtgttt ttaccggcac
    10801 aggtaccgga ttcagagctt gatcagtgga tggaaagccg gatttacccg gcgatggcgg
    10861 cgatccctgc actggcaggc atgattacca cgatggttac gcagggctat gagtatcgtc
    10921 gtgatgacga tatggcattg tggagctctg cagatctgac ttattccatt acatacgaga
    10981 tgtgaggatg atatggcaac accaaatccc ctggcgccgg taaaaggttc cggcaccacg
    11041 ctctgggttt acaccggtaa gggcgatgct tatgcaaacc cgctgtcaga tgatgagtgg
    11101 acgcgtctgg caaaaataaa ggatctgacc cccggcgaga tgacggcgga atcctacgac
    11161 gataactatc tggatgatga ggatgccgac tgggtatcca ccgggcaggg gcagaaatcc
    11221 gccggtgaca ccagttttac gctggcctgg aagcccggcg agaaagggca gcgtgatttg
    11281 attgcctggt ttgacagcag tgagacccgg gcctacaaaa tccgcttccc gaacggtacg
    11341 gtggatgtgt tccgtggctg ggtgagcgcc attggtaaag cggtgaccgc caaagaagtg
    11401 attacccgta cggtaaaaat caccaatatc ggtcgtcctt cgctggcgga ggatcgggga
    11461 gaaatcacac cggtcaccgg tattaccgtg acgccactaa cggggaatgt ggcaaaaggt
    11521 cagagcacca ccctgacagt ggccgttcag ccggaaggcg caacggataa aacattccgc
    11581 gcaacgtcgg caaatcagaa tttcgccacc attaccgtga aagggaacac gatcaccgtg
    11641 aaaggtgttg cggcaggtaa agcgcagatc cctgttgtca ccggcaacgg tgagtttgcg
    11701 gcggtggcgg agatcaccgt cacggatggc gctgcgggct gaggggagga gatcaagcat
    11761 gttcctgaaa acagaacaat ttgaatataa cggtgtgtcc gtcacgctgt cggagctgtc
    11821 cgcgctgcag cggtttgatt atatgaagtt tgtttcagac gcagaacaac aggggacaac
    11881 gaagcatgat gtcgtgcaca ttaaccagcg atatctggaa acggcatccc tgcttgtggc
    11941 gatgtcgcta tggcattccc attccctcaa aggcactctg gcctctccgg agacagagat
    12001 gcagcagatc cgccgtgaag tgatgctggg atggcctgct gatgcactga atcaggcaac
    12061 gaaccgggtg ctttatcttt caggtatgct ggataaccgg cacgatgccg atcctgaaca
    12121 aaccgggaaa gcagaagcga ctgagccggt aacatcaaaa aagcattcga aggcgagctg
    12181 aactttgtcc tgaaactggc gcgagagatg gggagacccg actggcgcgc catgcttgcc
    12241 gggatgacat ccaccgaata tgccgactgg cgacgttttt actgcacgca ttattttcag
    12301 gatacccaac tggacgctca tttttccggg ctgatgtacg ccgtactcag cctgtttttt
    12361 ggcgatccgg atatgcatcc ggcggatttc agtctgcttg cgccagtgtg tgaggaagag
    12421 cagacggaga tgccggacga ggaagacatg ctgatgcaga aagcggcagg agttgccgga
    12481 ggcgtccggt tctgtggggt gggtgggggc gatatttcac cttctgcgga tgtggtggat
    12541 gtcagcgaag atgatgttgc attaatgatg gcttcagcgg ggatttccgg aggtgtgaga
    12601 tatgtcccag ccagcgggtg atcttgttat tgatttgagt ctggatgcgg cccggtttga
    12661 tgaacagatg gcccgggtac gccgccattt ttccagtctg gaggcggatg ccagaaaaac
    12721 cgccagtact gttgaacagg g
//
LOCUS       DAZGQL010000127        12684 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.127, whole genome shotgun sequence.
ACCESSION   DAZGQL010000127 DAZGQL010000000
VERSION     DAZGQL010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12684
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.127"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..2101
                     /locus_tag="ACHKIR_004442"
     CDS             <1..2101
                     /locus_tag="ACHKIR_004442"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992090.1"
                     /translation="PVSVMAKAQKGSVINSITFTDSLTDETDDKHDFTNTGDLKGSTP
                     SIMTFPYGESDDRYTTNYVGNVQSSKTKFINLATGLTNDTTPTILISLDKELNNNQHI
                     EITRYKVDVDNDNILYEVVDEIIPSEHVDIKGKNIIVKDQLEHTYSQYYKYEFVIKDN
                     VDGKESVTSEKEFYFLLDTDVEAFDIHKIDKTKDNILFSGTGENNTQVMIKYKTERGE
                     EKNIKVVVDDTGKYEINLNGWDIKDADGAEVKIVDSAGNVKSGNLYNIARLYVDMNTN
                     KAITLDSAYNIIGSQKEGTDPAKALIMSDDNDWVYIGGGISPHIGDQNPGSDNNIDMA
                     GGDDILSSVGAVLDGANINMGDGDDKLYTQDGFASSGTRNIIMGDGNDVISVDNSFGG
                     KNTISLGEGNNLFIVGNYVNSIAENDITAGSGDDRIEIGTNLDGKNKIDLGDGDNTIQ
                     VGGYITNSHTITGNSGDDIIYVATNIDGSGSFNLGEGNNNFIVGGYIQGKNTIEMGSG
                     DDTVSVSTRIADNVKIQLNAGDDSVYAGGLLNKAIVDLGDGDDVVTLSGISDNGKRNN
                     MEELVSTNAMLTGGEGNDTLKINGSFKLLNMKNISGFETIDLGESSENHLDVGIKSDM
                     LDISSSSGVKIFTIMGGAGNTVDLGKANITSHNSVEQGNYADSWYKGDTVDGYTTYTP
                     VGDKSVELHIQQDILVI"
     gene            2447..3430
                     /locus_tag="ACHKIR_004443"
     CDS             2447..3430
                     /locus_tag="ACHKIR_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HFO9992091.1"
                     /translation="MPEKYSPDDQSSTIDPYLITSVRQTLEEQGAALQNLSKQLDSGQ
                     YQCVLNLIMNCKGHVILSGMGKSGHVGRKMSATLASTGTPSFFIHPAEAFHGDLGMIT
                     PCDLLILISASGETDEILKLVPSLKNFGNRIIAITNNGNSTLAKNADAVLELHMANET
                     CPNNLAPTTSTTLTMAIGDALAIAMIHQRKFMPNDFARYHPGGSLGRRLLTRVADVMQ
                     HDVPAVQLDASFKTVIQRITSGCQGMVMVEDAEGGLAGIITDGDLRRFMEKEDSLTSA
                     TAAQMMTREPLTLPEDTMIIEAEEKMQKHRVSTLLVTNKANKVTGLVRIFD"
     gene            3502..4650
                     /locus_tag="ACHKIR_004444"
     CDS             3502..4650
                     /locus_tag="ACHKIR_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis protein"
                     /protein_id="HFO9992092.1"
                     /translation="MLIKVKSAVSWMRAHLSAISLSDIQKHLVKIIILAPMAVLLIYL
                     AIFSQPRYMSESKVAIKRSDDLNSGSLNFGLLLGASNPSSAEDALYLKEYINSPDMLA
                     ALDKQLNFREAFSHSGLDFLNHLSKDETAEGFLKYYKDRINVSYDDKTGLLNIQTQGF
                     SPEFALKFNQTVLKESERFINEMSHRIARDQLAFAETEMEKARQRLDASKAELLSYQD
                     NNNVLDPQAQAQAASTLVNTLMGQKIQMEADLRNLLTYLREDAPQVVSARNAIQSLQA
                     QIDEEKSKITAPQGDKLNRMAVDFEEIKSKVEFNTELYKLTLTSIEKTRVEAARKLKV
                     LSVISSPQLPQESSFPNIPYLIACWLLVCCLLFGTLKLLLAVIEDHRD"
     gene            4674..6350
                     /locus_tag="ACHKIR_004445"
     CDS             4674..6350
                     /locus_tag="ACHKIR_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HFO9992093.1"
                     /translation="MKLFKSILLIAACHAAQASAAIDISADPNLTGAAPLTGILNGQK
                     SDTQNMSGFDNTPPPAPPVVMSRMFGAQLFNGTSADSGATVGFNPDYILNPGDSIQVR
                     LWGAFTFDGALQVDPKGNIFLPNVGPVKVAGVSNSQLNALVTSKVKEVYQSNVNVYAS
                     LLQAQPVKVYVTGFVRNPGLYGGVTSDSLLNYLIKAGGVDPERGSYVDIVVKRGNRVR
                     SNVNLYDFLLNGKLGLSQFADGDTIIVGPRQHTFSVQGDVFNSYDFEFRESSIPVTEA
                     LSWARPKPGATHITIMRKQGLQKRSEYYPISSAPGRMLQNGDTLIVSTDRYAGTIQVR
                     VEGAHSGEHAMVLPYGSTMRAVLEKVRPNSMSQMNAVQLYRPSVAQRQKEMLNLSLQK
                     LEEASLSAQSSTKEEASLRMQEAQLISRFVAKARTVVPKGEVILNESNIDSVLLEDGD
                     VINIPEKTSLVMVHGEVLFPNAVSWQKGMTTEDYIEKCGGLTQKSGNARIIVIRQNGA
                     AVNAEDVDSLKPGDEIMVLPKYESKNIEVTRGISTILYQLAVGAKVILSL"
     gene            6360..7100
                     /gene="kdsB"
                     /locus_tag="ACHKIR_004446"
     CDS             6360..7100
                     /gene="kdsB"
                     /locus_tag="ACHKIR_004446"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFO9992094.1"
                     /translation="MSKAVIVIPARYGSSRLPGKPLLDIVGKPMIQHVYERALQVAGV
                     AEVWVATDDQRVEKAVQAFGGKAIMTRNDHESGTDRLVEVMHKVEADIYINLQGDEPM
                     IRPRDVETLLQGMRDDPALPVATLCHAISAEEAAEPSTVKVVVNTHQDALYFSRSPIP
                     YPRNAEKARYLKHVGIYAYRRDVLQNYSQLPESMPEQAESLEQLRLMSAGINIRTFEV
                     AATGPGVDTPACLEKVRALMAQELAENA"
     gene            7097..9124
                     /locus_tag="ACHKIR_004447"
     CDS             7097..9124
                     /locus_tag="ACHKIR_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein"
                     /protein_id="HFO9992095.1"
                     /translation="MIGIFSSGIWRIPHLEKFLAQPCQKLSLLRPVPQEVDAIAVWGH
                     RPSAAKPVAIAKAAGKPVIRLEDGFVRSLDLSVNGAPPLSLVVDDCGIYYDASKPSAL
                     EKLVQDKAGNTALISKAREAMHTIVTGDLSKYNLAPAFVADESECSDIVLVVDQTFND
                     MSVTYGNAGQNEFSAMLEAAMAENPQATIWVKVHPDVLKGKKTGYFADLRATQRVRLI
                     TENISPQSLLRHISRVYVVTSQYGFEALLAGKPVTCFGQPWYAGWGLTDDRHPQSALL
                     SARRGSVTLEELFAAAYLRYCRYIDPLTGAASDLFTVLQWLQLLRNHQQQRNGYLWAP
                     GLTLWKSAILKPFLRTATNRLSYSRRCTAATACVVWGVKGEQQWQVEAQRKSLPLWRM
                     EDGFLRSSGLGSDLLPSLSLVLDKRGIYYDATRPSDLEVLLNHSQLTLAQQMRAEKLR
                     QRLVESKLSKYNLGADFSLPAEAKGKKVILVPGQVEDDSSIKTGTISIKSNLELLRTV
                     RERNPHAYIIYKPHPDVLVGNRKGDIPAELIAELADYQALDADIIQCIQHADEVHTMT
                     SLSGFEALLHGKHVHCYGLPFYAGWGLTFDEHHCGRRERELTLADLLYQALIVYPTYI
                     HPTRLQPITVEEAAEYLIQTSRKPMFITRKKAGWVIRYYRKLIMFCKVRFG"
     gene            9159..10358
                     /locus_tag="ACHKIR_004448"
     CDS             9159..10358
                     /locus_tag="ACHKIR_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001161852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HFO9992096.1"
                     /translation="MQGNALTVLLSGKKYLLLQGPMGPFFSDVAEWLESLGRNAVNVV
                     FNGGDCFYCRHRHYQAYYQTPKEFPGWLRDLHRQYDFDTILCFGDCRPLHKEAKYWAK
                     SKGIRFLAFEEGYLRPQFITVEEGGVNAYSSLPRDPDFYRKLPDMPAPHVENLKPSTM
                     KRIGHAMWYYLMGWHYRHEFPRYRHHKSFSPWYEARCWVRSYWRKQLYKVTQRKVLPR
                     LMNELDQLYYLAVLQVYNDSQIRNHSNYNDVRDYINEVMYSFSRKAPKESYLVIKHHP
                     MDRGHRLYRPLIKRLSKEYGLGGRVIYVHDLPMPGLLRHAKAVVTINSTVGISALIHN
                     KPLKVMGNALYDIKGLTYQGHLHQFWQADFKPDMKLFKKFRGYILMKTQLNAVYYGIF
                     NFRKITN"
     gene            11175..11951
                     /locus_tag="ACHKIR_004449"
     CDS             11175..11951
                     /locus_tag="ACHKIR_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO9992097.1"
                     /translation="MKRSGFEVQKVTVEALFLREIRTRFGKFRLGYLWAILEPAAHLL
                     VLLGIFGYIMHRTMPGISFPVFLLNGLIPFFIFNSISNRSVEAIDANQGLFNYRPVKP
                     MDTIIARTLLETLIYIIVYVLLMLIVWMSGEYFEIISLLQLVLVWTLLIIISCSVGLI
                     FMVVGKTFPEMQKILPIFLKPLYFISCIMFPLHSIPQQYWTYLLWNPLVHVVELSREA
                     VMPGYISEGVSLNYLAMFTLVTLFIGLALYRTREEAMLTS"
     gene            11948..12619
                     /locus_tag="ACHKIR_004450"
     CDS             11948..12619
                     /locus_tag="ACHKIR_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO9992098.1"
                     /translation="MIKIENLTKSYRTSTGRHYVFKDLNIELPSGKSVAFIGRNGAGK
                     STLLRMIGGIDRPDRGKIITNKTISWPIGLAGGFQGSLTGRENVKFVARLYAKPEELK
                     DKIEFVEEFAELGKYFDMPIKTYSSGMRSRLGFGLSMAFKFDYYIVDEVTAVGDARFK
                     EKCAHYFKERHKESSFLMVSHSLNSLKEYCDVVIYIKSFNEVKYYSVVTDALAEYIID
                     TKIQV"
BASE COUNT         3558 a         2586 c         3081 g         3459 t
ORIGIN      
        1 cccagtcagt gtgatggcaa aagcacagaa aggtagcgtc attaattcca ttaccttcac
       61 tgatagttta actgatgaaa ccgatgataa acatgatttt actaatacag gtgatctgaa
      121 gggcagtacg ccttctatca tgacatttcc gtatggtgaa tcagatgata ggtatacgac
      181 taactatgtt ggtaatgttc agtcctcaaa aacaaaattt ataaatcttg caacagggtt
      241 aacgaatgat actacgccaa ctatattaat atctcttgat aaggagctga ataataatca
      301 acatattgaa attacacgtt ataaagttga tgttgataac gataatatat tgtatgaagt
      361 agttgatgag ataatcccta gtgaacatgt ggatattaaa ggaaaaaata ttattgttaa
      421 agatcaactg gagcatacct atagtcaata ttataaatat gagtttgtca taaaagataa
      481 tgttgatggt aaagaatctg tgacttcgga gaaagagttt tacttcctgt tagatacaga
      541 tgttgaagca tttgatattc acaaaattga taaaactaaa gacaatatat tattttccgg
      601 tacaggtgaa aataatacgc aagtgatgat taaatataaa acagaacgtg gggaggaaaa
      661 aaatataaaa gtagttgttg atgacacagg aaagtatgaa ataaatctaa atggctggga
      721 catcaaggat gcagatggcg ctgaagtaaa aattgttgat tctgctggta acgttaaatc
      781 aggaaactta tataatatcg cgcgtttgta tgtggatatg aatacaaaca aagctataac
      841 gctggattcg gcttataata taattggctc tcagaaagaa ggaaccgatc ctgctaaagc
      901 tcttatcatg tcagatgata atgactgggt gtatattggt ggagggatat ctccccatat
      961 aggggatcag aatccaggct ctgacaacaa cattgatatg gctggcggtg atgatattct
     1021 gtcatctgtt ggtgcagttt tggatggagc caatattaat atgggtgatg gagatgataa
     1081 actatatacg caggatggtt tcgcttcttc aggtacccga aatattatta tgggagacgg
     1141 gaatgacgtt atttctgttg ataattcatt tggtggaaaa aataccattt ctttaggaga
     1201 gggaaacaac ttatttattg ttggtaacta tgtaaatagt attgctgaaa atgatattac
     1261 ggctggtagt ggtgatgaca gaatagaaat tggtacaaat ctggatggta aaaacaaaat
     1321 tgatcttggg gatggtgaca acactattca ggttggagga tacataacca actcacatac
     1381 cattactggg aatagtggtg atgatattat ctatgtcgct acaaatatag atggttctgg
     1441 ctcatttaat ttaggtgaag gtaataacaa ctttatagtc ggaggatata tccaggggaa
     1501 aaacacaatt gagatgggtt ccggtgatga tacggtttct gtttccactc gtattgcaga
     1561 taatgttaaa atccaactta atgccgggga tgacagtgtg tatgcaggtg gattgttaaa
     1621 caaggcaatt gttgacctcg gggatgggga tgatgtcgtc acattgagtg gaatatcaga
     1681 taatggtaag cgtaataata tggaggagtt agtatccact aatgcaatgc ttacaggagg
     1741 ggaaggaaac gatactctga agattaatgg ttctttcaaa ttgttaaata tgaaaaatat
     1801 atctgggttt gaaactattg atcttgggga aagttcagag aatcatctgg atgtcggtat
     1861 aaaatcggat atgctggata tttcatcgtc atctggggtg aaaattttta ctatcatggg
     1921 tggggcggga aatacagttg atttaggtaa ggcaaatata acgtctcaca attcagtaga
     1981 acaaggaaat tatgctgatt catggtataa aggagatact gtggacggat acacaacgta
     2041 tactcctgta ggtgataaat ctgttgaact ccatatccaa caggatatat tagtaatata
     2101 attttaatag cataaaagtg gtcgcatatg tgaccacttt tatttgttgg ctttagttat
     2161 ttcaggtttt attaagaaag aagctaataa tatacatcat gttgttattt gttggtttaa
     2221 tgttaacatg tatgataaaa atcatgtggg acgggcatgt gcccctccct ttcctttatt
     2281 atgtctgata atgaaatgct aaaattattt ttgtttgtag actgtgttat atgctttatg
     2341 gaaaagtagt attttgattt atgtgaagat gagctgaact cgatttatca gaggcaagtt
     2401 gtctttatgt tataagtgtt ttaaccttat cggaagtata atttatatgc cagaaaaata
     2461 ttcacctgat gatcagagca gtactattga tccatattta attacttctg ttcgtcagac
     2521 cttggaagaa caaggtgcag cattacaaaa cttgtctaaa caactggatt ccgggcagta
     2581 ccagtgtgtc cttaatttga taatgaactg taaagggcac gttattcttt cgggaatggg
     2641 taaatcaggg catgtcggtc gtaaaatgtc agcgacgctg gcctctaccg gtaccccgag
     2701 tttctttatt catcctgcag aagcgttcca tggcgatctg ggtatgatta cgccctgcga
     2761 tcttttgatc cttatttctg ccagcggtga aacggatgaa atcctcaagc tagttccttc
     2821 actgaaaaat ttcggcaacc gaattatcgc cattaccaat aatggaaatt ccacgctggc
     2881 gaaaaatgct gatgccgtgc tggaactcca tatggcgaat gaaacctgcc cgaataatct
     2941 tgcaccaaca acgtctacca ccctgaccat ggcgatcggc gatgcgctgg cgattgccat
     3001 gatccaccaa cgcaaattta tgccgaatga ttttgcgcgc tatcacccgg gcggttcatt
     3061 aggtcgtcgc ctgctgaccc gcgttgctga tgtcatgcag catgatgttc ctgcggtaca
     3121 gctggatgcg tcatttaaaa ccgtgattca acgtatcacc agcggatgcc agggaatggt
     3181 gatggtagaa gacgcagaag gtgggctggc gggcattata accgacggtg acctgcgtcg
     3241 ctttatggaa aaagaggatt ctctgacatc cgctacggct gcgcagatga tgacacgtga
     3301 accgctgacg ctaccggaag acaccatgat cattgaagcg gaagaaaaaa tgcaaaagca
     3361 ccgcgtctca acgttattgg tgaccaacaa ggcaaataaa gtcactggcc ttgtgcgcat
     3421 tttcgactaa ttaagcaacg gggtttgaga ggttagtctc tcattgagtt tgtgccccac
     3481 tggaactttg ataatcgtta catgttgata aaagtgaagt ctgccgtatc ctggatgcgt
     3541 gctcatctgt ctgccatctc actgtcagat atccaaaaac acctggtgaa aatcatcatt
     3601 ctggcaccga tggcggtgct gctgatctat ctggctatct tcagccagcc tcgctatatg
     3661 agcgagtcga aagtcgccat taaacgctcg gatgatttaa acagcggcag cctgaatttt
     3721 ggtctgcttc tgggtgcctc taaccccagt tccgcagaag atgcgttgta tctgaaagag
     3781 tacatcaact cgccggatat gctggcggcg ctggataaac aactaaattt tcgtgaagcg
     3841 ttcagccaca gcgggctcga ttttcttaat catcttagca aggatgaaac cgcagaaggc
     3901 ttcctgaagt actacaagga ccgtatcaac gtctcgtatg acgataaaac cggattactg
     3961 aatattcaga cgcagggctt tagcccggag tttgcgctta agtttaacca gaccgtgctg
     4021 aaagagtcag agcgctttat caatgagatg tcacatcgca tcgcgcgtga ccagcttgcc
     4081 tttgcagaaa cggagatgga aaaggcacgc cagcgtctgg acgccagcaa agcggaattg
     4141 ctctcttatc aggacaacaa caacgttctg gatccacagg cacaggcaca ggcggcgagc
     4201 acgttagtca atacgctgat gggccagaag atccagatgg aagcggatct gcgtaacttg
     4261 ctgacgtatc tgcgtgagga cgccccgcaa gtggtgagtg cgcgtaatgc gattcagtca
     4321 ttacaggcac aaatcgatga agaaaaaagc aaaatcactg caccacaggg tgacaagcta
     4381 aaccgtatgg cggtggattt tgaagaaatc aaatcaaaag ttgagttcaa caccgagctc
     4441 tacaaactga ccctgacctc cattgaaaag acccgtgtag aagcagctcg taagctcaag
     4501 gtgctgtcag tgatcagttc gccacagttg ccgcaggaat cgtcttttcc aaatatccct
     4561 tatttgatcg cctgctggtt actggtgtgc tgcctgctgt tcggcaccct gaaactgttg
     4621 ctggctgtca ttgaagatca ccgagactaa cgctgtcgct gaatgagttt gtgatgaaat
     4681 tatttaaatc aattttactg attgccgcct gtcacgcggc gcaggccagc gcggccattg
     4741 atattagcgc tgacccaaac ctgacaggag ccgcgccgct taccggtatt ctgaacggac
     4801 agaagtcgga tacgcaaaac atgagcggct tcgacaatac cccaccgccc gcaccgccgg
     4861 tggtaatgag ccgtatgttt ggtgctcaac ttttcaacgg caccagcgcg gatagcggtg
     4921 cgacggtagg attcaaccct gactatattc tgaatccggg tgatagcatt caggttcgct
     4981 tgtggggtgc gttcaccttt gatggtgcgt tacaggttga tcccaaaggt aatattttcc
     5041 tgccgaacgt tggtccggtg aaagttgctg gcgtcagtaa tagtcagcta aatgccctgg
     5101 tcacatccaa agtgaaggaa gtataccagt ccaacgtcaa cgtctacgcc tccttattac
     5161 aggcgcagcc agtaaaagtg tacgtgaccg gatttgtgcg taatcctggt ctgtatggcg
     5221 gtgtgacgtc tgattcgtta ctcaattatc tgatcaaggc tggcggcgtt gatccagagc
     5281 gcggaagtta cgttgatatt gtggtcaagc gcggtaaccg cgtgcgctcc aacgtcaacc
     5341 tgtacgactt cctgctgaac ggcaaactgg ggctttcgca gttcgccgat ggtgacacca
     5401 tcatcgtcgg gccgcgtcag catactttca gcgttcaggg cgatgtcttt aacagctacg
     5461 actttgagtt ccgcgaaagc agcattcccg taacggaagc tttaagctgg gcgcgcccta
     5521 agcctggcgc gactcacatt acgattatgc gtaaacaggg gctgcaaaaa cgcagcgaat
     5581 actatccgat cagttctgcg ccaggtcgta tgttgcaaaa tggcgatacc ttaatcgtga
     5641 gcactgaccg ctatgccggt accattcagg ttcgggttga aggcgcgcac tccggtgaac
     5701 atgccatggt actgccttat ggttccacta tgcgtgcggt tctggaaaaa gtccgcccga
     5761 acagcatgtc gcagatgaac gcggttcagc tttatcgccc atcagtggct cagcgtcaga
     5821 aagagatgct gaatctctcg ctgcaaaaac tggaggaagc atcactttct gcccagtcct
     5881 ccaccaaaga agaagccagc ctgcgaatgc aggaagcaca actgatcagc cgctttgtgg
     5941 cgaaagcacg caccgtggtt ccgaaaggtg aagtgatcct caacgaatcc aatattgatt
     6001 ctgttctgct tgaagatggc gacgtcatca atattccgga gaaaacatcg ctggttatgg
     6061 ttcatggcga agtgctgttc ccgaacgcgg tgagctggca gaagggtatg accaccgagg
     6121 attacatcga gaaatgtggt ggcctgacgc aaaaatcggg taacgccaga attatcgtca
     6181 ttcgtcagaa cggtgctgcc gtcaacgcgg aagacgtgga ttcactcaag ccgggcgatg
     6241 agattatggt tctgccgaaa tatgaatcga aaaatattga agttacccgt ggtatttcca
     6301 caatcctcta tcagctggcg gtgggtgcaa aagtgattct gtctttgtaa ggagttgaga
     6361 tgagcaaagc agttattgtc atcccggctc gctatggctc ctcgcgcctg ccgggtaagc
     6421 cactgctcga tattgttggt aaaccgatga tccagcatgt ttacgagcgt gcgttacagg
     6481 tggcgggcgt cgcagaagtt tgggtggcaa cagacgatca gcgtgttgaa aaggccgtgc
     6541 aggcatttgg cgggaaagcc atcatgacgc gcaacgatca tgaatccggc accgatcggc
     6601 tggtcgaggt gatgcataaa gtcgaggcag atatctacat taacctgcag ggcgacgaac
     6661 caatgattcg cccgcgggat gtagaaacgc tgttacaagg aatgcgtgat gaccccgcgt
     6721 tgccagtggc aacgctatgc cacgcgattt ctgccgaaga agcggctgag ccaagcacgg
     6781 taaaagtggt ggtaaatacc caccaggatg cgctttattt cagccgttcg ccgattccgt
     6841 atccgcgtaa tgctgaaaaa gcgcgctacc tgaaacacgt tggtatttac gcttatcgtc
     6901 gcgatgtgct gcaaaactac agccagttac cggagtccat gccagagcag gcagaatcac
     6961 tggagcagct gcggttaatg agcgcgggga tcaacatccg cacatttgag gttgccgcaa
     7021 ccggtccggg cgtcgacacc ccagcatgcc tggaaaaagt gcgcgccctg atggcacagg
     7081 aactggctga aaacgcatga ttggcatttt ctcgtccggt atctggcgta ttccgcatct
     7141 ggagaaattt ctggcgcaac cgtgccagaa actttctctg ctgcgccctg ttccgcaaga
     7201 agttgatgct atcgccgtgt ggggacatcg tcccagcgcg gcaaaaccgg tcgccatcgc
     7261 caaagcagcg ggaaaacccg tcatccgtct ggaagatgga tttgtgcgtt cgctggatct
     7321 tagcgtcaat ggtgcgccgc cgctttctct cgtggtggat gattgtggca tttactacga
     7381 tgccagcaag ccttcagcac tggagaaact ggtacaggat aaagccggaa atacagctct
     7441 gataagtaag gccagagagg cgatgcacac catcgtgacc ggggatttgt cgaaatataa
     7501 cctggcgcct gcgtttgtgg ctgatgagtc agaatgttca gacatcgttc tggttgtcga
     7561 tcagacattt aatgatatgt cagtgacgta tggtaatgct ggtcagaatg agttttctgc
     7621 catgctggag gccgcgatgg cggaaaatcc tcaagcgaca atttgggtga aggtgcaccc
     7681 agatgtactg aaaggaaaga aaacaggtta tttcgccgat ctgcgcgcca cgcaacgagt
     7741 acgtttgatt acggagaata tcagtccgca gtcgctgttg cgacatattt ccagagttta
     7801 cgtcgtgacc tcccaatacg gctttgaggc cttactggca ggaaaaccag tgacatgctt
     7861 cggtcaaccc tggtatgcag gctggggctt aacggacgat cgtcatccgc agtcggcttt
     7921 gttatctgcc cgacgcggtt ctgtcacgct ggaagaactt tttgccgcag catacctgcg
     7981 ttactgtcgc tatatcgacc cactaacggg ggcagcaagt gacctgttta ccgtactgca
     8041 atggctgcaa ttactgcgca accatcagca acagcgtaat ggctatttat gggcgccagg
     8101 cttaacgctg tggaagtcag caatcctgaa acctttcttg cgtaccgcga caaacaggct
     8161 gagttattca cgtcgctgta ctgctgccac cgcctgcgtg gtatggggtg taaaaggaga
     8221 acagcaatgg caggtcgaag cgcagcgaaa atcactgcca ttatggcgaa tggaagatgg
     8281 ttttctgcgt tcatccggac ttggctcgga cctgctgcca tcgctatcgc tggtgctgga
     8341 taaacgcgga atttactatg acgccacgcg ccccagcgac ctggaagtgc tgcttaatca
     8401 cagccagtta acgctggcgc agcagatgcg agctgagaag ttgcggcaac gactggttga
     8461 aagcaaactg agtaagtaca acctgggagc cgatttctct ctgcctgccg aagccaaagg
     8521 taaaaaagtt atcctggtgc cgggtcaggt agaggacgat tcctctatta aaacaggcac
     8581 tatctcaatt aagagcaacc ttgagttatt acgcacagta cgcgagcgta atccgcacgc
     8641 ctacattatt tataaaccgc acccggatgt actggtgggg aatcgcaagg gcgatattcc
     8701 ggcagaactg attgctgaac ttgctgatta tcaggcactg gacgcagata ttattcaatg
     8761 cattcagcac gcagatgaag tgcataccat gacgtcgctg tcggggtttg aagcgttatt
     8821 acatggtaag cacgtacatt gttacggcct gcccttctat gctggttggg gtttaacctt
     8881 cgatgaacat cactgcgggc gtcgtgagcg agaattaacg ttagcggatt tgctatatca
     8941 ggcgctgatt gtttatccaa cttatatcca cccaacacgg ctacaaccta ttactgttga
     9001 agaggcagcg gaatatttga tccagacgtc gcgcaagccg atgtttatta cccgaaaaaa
     9061 agcgggatgg gtaatacgct attaccgcaa attaattatg ttctgcaagg ttagatttgg
     9121 ctaaacaatt tcatagttga ctattacgca aattatttat gcaaggtaat gcactaaccg
     9181 ttttattatc cggtaaaaaa tatctgctat tgcagggacc gatgggaccc tttttcagtg
     9241 atgtagccga gtggctggag tcattaggcc gtaacgctgt gaatgttgta ttcaatggag
     9301 gggattgttt ttactgtcgc caccgacatt atcaggctta ctaccagaca ccgaaagagt
     9361 ttcccggttg gttacgggat ctccaccggc aatatgactt tgataccatc ctctgctttg
     9421 gtgactgccg cccattacac aaggaagcaa aatattgggc aaagtcgaaa ggtatccgct
     9481 ttctggcatt tgaggaaggg tatttacgcc cgcagtttat taccgttgaa gaaggcggag
     9541 ttaacgcata ttcatcgcta ccgcgcgatc cggattttta tcgtaagtta ccagatatgc
     9601 ctgcgccgca cgttgagaac ttaaaacctt caacgatgaa acgtataggt catgcgatgt
     9661 ggtattacct gatgggctgg cattaccgcc atgagttccc tcgctaccgc caccataaat
     9721 cgttttcccc ctggtatgaa gcacgttgct gggttcgttc atactggcgc aagcaacttt
     9781 acaaggtaac acagcgtaag gtattgccga ggttaatgaa cgagttggac caactttatt
     9841 atcttgccgt tttgcaggta tataacgata gccagattcg taaccacagt aattataacg
     9901 atgtgcgtga ctatattaat gaagtcatgt actcattttc acgtaaagcg ccgaaagaaa
     9961 gttatttggt gatcaaacat catccgatgg atcgtggtca cagactctat cgaccattaa
    10021 ttaagcggtt gagtaaggaa tatggcttag gtgggagagt catttatgtg cacgatctcc
    10081 cgatgccggg actattacgc catgcaaaag cggtggtgac aattaacagt acggtgggaa
    10141 tctctgcact gattcataac aaaccactta aagtgatggg caatgccctc tacgacatca
    10201 agggcttgac atatcaaggg catttacacc agttctggca ggctgatttt aaaccagata
    10261 tgaaactgtt taagaagttt cgtggatata tattgatgaa gacacagctt aatgcagttt
    10321 attatgggat ttttaatttt agaaaaataa cgaattaata aaacatctat aatacaaaca
    10381 acggttgttg tatattattt attgaattca atcattttta tacttaatgt ttttgtaaat
    10441 aataagttga ctgtgttgta ctatattaat ataggcgttt atattcatat ctttaagagt
    10501 aagctattag atgtaaatta ctttggtcag gtccttcaca gcagaaacag aatgtcatga
    10561 aagataaatt tacgcagtca cttgatcaac tgcttagttt tgcgtcttaa aggattgtaa
    10621 ctatggcaac tgtggagcga aaaatcattg ggcacgggaa aagtgcggtc atatagaaaa
    10681 gtgctctcta ttcagcatct catggtttat cagcgaagac agaaaaaaat tataacgatg
    10741 cgcaggaatt tgtggaggtt tgctaatata gagcgattcg agcatccctc gttaaatctc
    10801 tcgaaccgca gtatattcag acatttttaa aatacgccag atgattacga cagaaagaac
    10861 acaacatata attcatgttc gctgtcctct tgctgaatac ggaattgtct tcaataaagg
    10921 tgctacacat tatggtgaga gctaccacgg tttcttgagg atgcagaaaa tggatttacc
    10981 gatatcgtgc aattttgtat cggtagtatg tccgactcat gtctcatgga taatgaacca
    11041 tatattataa ctgcgggtag aaaaatgcgc atctaacgac tttgcctgga cgtgtcgatt
    11101 aactgttcct gggtttgcta ctcttgtcag tcagacgata aaagtgagca taaatatata
    11161 ggattacata tcaagtgaaa agaagtggat ttgaagttca gaaggtgaca gtagaggcat
    11221 tatttctacg agaaatacga acacgttttg gtaagtttcg tctgggatat ctgtgggcaa
    11281 tcctggaacc cgctgctcat ttgctggtgc tgttaggtat ttttggctat attatgcatc
    11341 gtacgatgcc aggtatctcg ttcccggtat ttttacttaa tggtttgatt ccctttttta
    11401 tctttaatag tatcagtaat cggtctgtgg aagctattga tgcaaaccag gggcttttta
    11461 attatcggcc agtaaagccc atggatacaa taatagccag aaccctgctt gagacactaa
    11521 tttacattat agtttatgtg ttgcttatgc ttattgtctg gatgtcagga gaatattttg
    11581 aaataataag tttattacaa ctggtacttg tatggactct tctgattatt atttcatgta
    11641 gtgtcggatt aatatttatg gtcgttggta aaactttccc tgaaatgcaa aagattctac
    11701 cgatatttct taaaccgctg tatttcattt cctgtatcat gttccctcta cattcgatcc
    11761 cacagcaata ctggacatat ctactctgga acccattagt ccatgtcgta gagttaagtc
    11821 gtgaggcagt tatgccgggc tatatcagcg aaggcgtgag tttgaactat ctggcaatgt
    11881 tcactctggt caccctgttc atcggtctgg cgctataccg aacgcgtgaa gaggcaatgc
    11941 tgacatcatg attaagattg agaatctgac gaagtcatat cgcacgtcaa caggtcgaca
    12001 ctatgtgttt aaggatttaa atatcgaact cccttcagga aaaagtgttg cctttattgg
    12061 tcgtaatggt gcaggtaaat caactttact gagaatgatt ggcggtattg accgccccga
    12121 tagaggaaag atcatcacca ataaaacgat atcatggccc attgggctcg ccggtggatt
    12181 ccagggcagt ttaaccgggc gagaaaacgt aaaatttgtt gcacggttat atgcgaagcc
    12241 agaagaactg aaagataaaa ttgagtttgt cgaagaattt gccgaactcg gtaagtactt
    12301 tgatatgccg atcaaaactt actcatctgg tatgcgatct cgcctgggtt ttggtttaag
    12361 catggcattt aagtttgatt attatattgt cgatgaagta acggcagtcg gtgatgccag
    12421 gtttaaagaa aaatgtgcac attactttaa agaaaggcat aaagagtcta gttttttaat
    12481 ggtttctcat agtttaaatt ctttgaaaga gtattgcgat gtggttatat atattaaaag
    12541 ttttaatgaa gtaaaatact acagtgtagt aactgatgcg ctggcagaat atattatcga
    12601 cactaaaata caagtataaa ctttccaagt aacaaggcgt tattatgtct ataattgtaa
    12661 atcatgttat gtcttctgat atag
//
LOCUS       DAZGQL010000128        12631 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.128, whole genome shotgun sequence.
ACCESSION   DAZGQL010000128 DAZGQL010000000
VERSION     DAZGQL010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12631
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.128"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            20..1153
                     /gene="ompN"
                     /locus_tag="ACHKIR_004451"
     CDS             20..1153
                     /gene="ompN"
                     /locus_tag="ACHKIR_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HFO9992099.1"
                     /translation="MKSKVLALLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSTNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLVNGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGLSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHAAGGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
     gene            1294..1728
                     /gene="uspF"
                     /locus_tag="ACHKIR_004452"
     CDS             1294..1728
                     /gene="uspF"
                     /locus_tag="ACHKIR_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HFO9992100.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            1904..2839
                     /gene="ttcA"
                     /locus_tag="ACHKIR_004453"
     CDS             1904..2839
                     /gene="ttcA"
                     /locus_tag="ACHKIR_004453"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFO9992101.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLETLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(2968..4341)
                     /gene="dbpA"
                     /locus_tag="ACHKIR_004454"
     CDS             complement(2968..4341)
                     /gene="dbpA"
                     /locus_tag="ACHKIR_004454"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HFO9992102.1"
                     /translation="MSAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPTILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRMLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQSVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPVNSSIVPLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     CRVRLLK"
     gene            complement(4371..4544)
                     /gene="ynaL"
                     /locus_tag="ACHKIR_004455"
     CDS             complement(4371..4544)
                     /gene="ynaL"
                     /locus_tag="ACHKIR_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaL"
                     /protein_id="HFO9992103.1"
                     /translation="MTTLIYLQIPVPEPIPGDPVPVPDPIPRPQPMPDPPPDEEPIKL
                     SHRERRSARIRAC"
     gene            complement(4819..5802)
                     /gene="zntB"
                     /locus_tag="ACHKIR_004456"
     CDS             complement(4819..5802)
                     /gene="zntB"
                     /locus_tag="ACHKIR_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071577 - zinc ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HFO9992104.1"
                     /translation="MEAIKGADVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            complement(5816..5863)
                     /gene="ydaN"
                     /locus_tag="ACHKIR_004457"
     CDS             complement(5816..5863)
                     /gene="ydaN"
                     /locus_tag="ACHKIR_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaN"
                     /protein_id="HFO9992105.1"
                     /translation="MLRNRARRLDYKIMR"
     gene            6057..7289
                     /gene="dgcM"
                     /locus_tag="ACHKIR_004458"
     CDS             6057..7289
                     /gene="dgcM"
                     /locus_tag="ACHKIR_004458"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HFO9992106.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRNEEETALSCRLVLRKLTEAEPVI
                     IFEGIEAPATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFVHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(7310..7873)
                     /gene="smrA"
                     /locus_tag="ACHKIR_004459"
     CDS             complement(7310..7873)
                     /gene="smrA"
                     /locus_tag="ACHKIR_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HFO9992107.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLTQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMV
                     FSFIQQALADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(8068..9582)
                     /locus_tag="ACHKIR_004460"
     CDS             complement(8068..9582)
                     /locus_tag="ACHKIR_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HFO9992108.1"
                     /translation="MMSMRKHIAFASMCMGLFIAQLDIQIVSSSLNEIGGGLSAGKDE
                     MAWLQTSYLIAEIIVIPLSGWLSRVFSTRWLFTLSAGIFTLMSIACGLAWNIQIMILF
                     RALQGAAGASMIPLVFTTAFIYYQGKELGSAAAVVSALASLSPTLGPTLGGWITDNLD
                     WRWLFYINILPGIYLVLSIPFLVNFDKPDLSLLKVADYPSIILLAMTLGCLEYTLEEG
                     ARWGWLDDNTILLTSVLALVSFILFAARTLTISNPIMDLHAFKDKNFTLGCFFSFSGG
                     VVIFSTVYLILVFLGQVRGLNAEEIGFAVCTTGIFQLFSVPFYFWLSKKINLRWLLMA
                     GLGGFVFSMYLFTPITHEWGWQELLFPQAIRGISQQFAMAPIVTLTLGGIPKERLKLA
                     SGVFNLTRNLGGAIGIALCGSILNNRTNFHFSRMGEKMVSVPHTVNDFISRSALFFNR
                     SGSDQTSEILASTKLLSQLMLREAQTMAFSDTFLLISGLLFIAFLLVPAMNKSS"
     gene            complement(9579..10607)
                     /locus_tag="ACHKIR_004461"
     CDS             complement(9579..10607)
                     /locus_tag="ACHKIR_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFO9992109.1"
                     /translation="MIISKKQLIGVVAIGIILAGVVFFIWWVSKGRFIQTTDDAYIGG
                     NITTVASKVSGYISAIEVRDNQSVKKGDIILRLDDRDYRANVARLEAKIKSRKANLES
                     IQATITMQQSIIQSASETWQAVKHEEQKRLRDTERYEKLVQSAAISQQIIDNARFDYQ
                     QVAAKERKAANDFLVEKQRLAVLSAQEENVRASIEEVQAALTQALLDLEYTLVRAPID
                     GIVANRSAHTGSWVEGGTSLVSLVPVSELWVDANYKENQIAGMKPGMKAEIRADILKG
                     EVFHGHIESLSPATGASFSLIPIENATGNFTKIVQRVPVRIAFDDAKELKQLLRPGLS
                     VTVSVDER"
     gene            10704..11519
                     /locus_tag="ACHKIR_004462"
     CDS             10704..11519
                     /locus_tag="ACHKIR_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707689.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO9992110.1"
                     /translation="MTITLQPKASPGHHIIGLDSEHLNGGTVPWHKHLYAQLLYPAEG
                     VVRVWAGESVWLVHASSALWLPPQMPHKFVATGNVLLKTVLVSEAESETLGKVCFMTG
                     ISPLLRELLIAINQLPPSQSTTDKQQLRFSALETLILQEIKMGVKMSLELPWPNDERL
                     QQLCENLLNNQGYLPTLDNLADKINVSSRTLMRLFVKETGLTFRHWVQQMHVISAVTL
                     LDDGYSLTKIAHRLGYASAESFGNMFKRRTGYSPGKFTRRLTMHDYAITRQMI"
     gene            complement(11693..11909)
                     /locus_tag="ACHKIR_004463"
                     /pseudo
     CDS             complement(11693..11909)
                     /locus_tag="ACHKIR_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885449.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            11909..12052
                     /locus_tag="ACHKIR_004464"
                     /pseudo
     CDS             11909..12052
                     /locus_tag="ACHKIR_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
     gene            12063..12578
                     /gene="ogt"
                     /locus_tag="ACHKIR_004465"
     CDS             12063..12578
                     /gene="ogt"
                     /locus_tag="ACHKIR_004465"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFO9992111.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISAANPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
BASE COUNT         3414 a         3135 c         2878 g         3204 t
ORIGIN      
        1 taactaaagg atttattcaa tgaaaagcaa agtactggca cttttaattc ctgccctgct
       61 cgccgcaggt gctgcacatg cagccgaagt ttataataaa gacggcaaca aattagatct
      121 gtatggcaaa gttgatggcc tgcattattt ttctgataat tcagcgaaag atggcgacca
      181 gagctatgcg cgtctgggtt ttaaaggcga aacccaaatt aacgatcaac tcactggcta
      241 cggtcaatgg gaatacaata ttcaggcaaa caacactgaa tcttcaacaa accagtcatg
      301 gacccgtctg gcatttgccg gactgaaatt tgcagattac ggttctttcg attacggacg
      361 taattatggc gtaatgtacg acatcgaagg ctggaccgat atgctgcctg aatttggcgg
      421 tgactcttat accaatgcag acaactttat gactggtcgt gccaacggcg tcgcgactta
      481 tcgtaatact gatttcttcg gtctggtaaa tggtctgaac ttcgcggtgc agtatcaagg
      541 taacaacgaa ggagccagta atggtcagga aggcaccaac aacggacgtg atgttcgcca
      601 tgaaaacggt gacggctggg gtctttccac aacatatgat ttaggcatgg gctttagcgc
      661 tggtgcggca tacacctctt ctgaccgcac caatgaccag gttaaccata ctgcggcggg
      721 tggtgataaa gcagacgcgt ggactgctgg gctaaaatac gatgctaaca atatttacct
      781 ggcaaccatg tattcagaaa cgcgtaacat gaccccgttt ggcgacagcg attatgcagt
      841 tgcgaacaaa acccagaact ttgaagttac tgcacagtac cagtttgatt ttggtctgcg
      901 tccggcagtc tctttcctga tgtctaaagg ccgtgacctg cacgctgccg gtggtgcaga
      961 caacccggca ggtgttgatg ataaagatct ggttaaatac gccgatgttg gcgcgactta
     1021 ctatttcaac aaaaatatgt ccacctacgt tgactataaa ataaacctgt tggatgaaga
     1081 tgacagcttc tacgctgcca acggcatctc taccgatgat attgtcgctt taggtctggt
     1141 ttatcagttc taaatcctcc tgcccgctgt tatggcgggc tttttctgct tattcttcct
     1201 cctttgatct aaattaaaaa tgtgaactcc gtcattacac aaaaagtgtc atctggcgtt
     1261 acactttatg cggatactaa aacaggaggt tttatgaaca gaacgattct tgtccctatc
     1321 gatatttccg attcagaatt aactcaacgc gtgattagcc acgttgaggc agaggcaaag
     1381 attgatgatg cagaggttca tttcctgacg gtaatacctt cactgcccta ctatgcctct
     1441 ctgggtttag cgtattccgc agaattaccg gcaatggatg acctgaaagc ggaagccaaa
     1501 tcgcaactgg aagagatcat taaaaaattt aaactgccaa ccgacagagt gcatgtccat
     1561 gttgaggaag gctcgcccaa agaccgcatt ctggaattgg cgaagaagat ccccgctcat
     1621 atgatcatca ttgcttccca tcgaccggat atcaccactt atctgctcgg ttctaacgcc
     1681 gcagcggtag tgcgtcacgc agagtgctcc gtgctggttg tgcgctgaca ttaacgcccg
     1741 cacattgctg cgggcttttt gattcatttc gcaaatgtgc tgacattttc ccctctaatc
     1801 cgtaccatac acgccacagt ttttatatca gatttcttat gctgggcgtt ccggcatgac
     1861 gcatactctt ctgatgccat ataacgaatt gagtcgcttt taaatgtcgc aaaatcaaga
     1921 aattagtaag aaagaacaat acaacctgaa caaattacaa aaacgtctgc gtcgtaacgt
     1981 gggcgaagcc attgctgact tcaatatgat tgaagaaggc gatcgcatca tggtttgcct
     2041 ctccgggggt aaagacagct ataccatgct ggaaattctg cgcaatttgc agcaaagcgc
     2101 gccaatcaat ttttcgctgg tggctgttaa cctcgatcaa aagcaaccgg gcttcccgga
     2161 acacgttctg cccgagtatc ttgaaacgct gggcgttgag tacaagattg tggaagagaa
     2221 cacttacggt atcgtgaaag agaagattcc ggagggcaaa accacttgct cactgtgttc
     2281 tcgtcttcgt cgcggtatcc tttatcgcac cgcaacggaa ctgggggcga cgaagatcgc
     2341 gctgggtcac catcgtgacg atatcctgca aacgttgttc ttaaatatgt tctacggcgg
     2401 caagatgaaa ggtatgcctc cgaaactgat gagcgatgat ggcaaacata ttgtgattcg
     2461 tccgctggcc tactgccgcg agaaagatat tcagcgcttt gccgatgcaa aagcgttccc
     2521 gattattccg tgcaacctgt gcggttcaca gcctaacctg caacgtcagg tgattgctga
     2581 catgttgcgt gactgggata aacgttatcc agggcgtatc gagacgatgt tcagcgcgat
     2641 gcagaatgtg gtgccgtcgc atctgtgcga taccaacctg ttcgatttca aaggtattac
     2701 ccacggttct gaagtggtta acgggggtga tctggcgttt gatcgcgaag agatccctct
     2761 acaaccggcg ggttggcagc ctgaagaaga tgaaaatcag ttggatgagt tacggctgaa
     2821 tgtggttgaa gtgaaataac ccggatagcg cccgatgcgc aagcttaccg ggctactctt
     2881 atggaggccg gataagacgc ggccagcgtc gcatccggca atcccgaaga agatgtttac
     2941 tcttgcaccc tgcgattcaa catttcatta ttttaataac cgcacccggc acgtttttcc
     3001 tttaatcttc ccgccctgta actgtttcca tgctttatga gcaacagcct gacgcaccgc
     3061 gacatagaca tgcgccggat gcacggcgat tttgccaata tctgcgccat caagcccgat
     3121 atctcctgtc agcgcaccta atacatcacc cgggcgcatt ttggcttttt tcccgccatc
     3181 gatacataac gttgccattt ctgcttccag cggcacaatg gaactattaa ctggcggcgt
     3241 ttgccagtta agttttatct gcaacatgtc agaaatgata ttggcccgct gtgcttcttc
     3301 cggggcacag aaactgatcg ccagaccgct atttcctgca cgagctgtac gaccgatgcg
     3361 atgtacatga acttcagggt cccacgccag ctcaaagttc accaccagct caagcgattt
     3421 tatatccaga ccacgcgcag caacatcagt cgcgaccagt acacgggcgc taccgttagc
     3481 aaaacgtacc agggtctgat cgcgatcgcg ttgctccaga tcgccgtgta acgacaatgc
     3541 actttgccct acttcattca gcgcgtcgca gacagactgg caatcttttt tggtattgca
     3601 aaacaccacg caagaggatg gctgatgcaa gcttaataac cgttgcaaca gaggaatttt
     3661 gcctttgctg gatgtctcat aaaattgttg ttcaataggt ggcagagcat ctgttgagtc
     3721 aatttcaatc gctaaaggat cgcgttgcac tcttccgctg attgcggcga tggcttccgg
     3781 ccaggttgcc gaaaacagaa gcgtctgtcg agatgctggc gcaaaacgga tgacatcatc
     3841 aatggcatcg ctaaatccca tatccagcat gcggtcggcc tcatccatca ccagcgtatt
     3901 caacgcatcc agtgataccg tgcctttttg cagatgatcc agcatacggc ccggcgttgc
     3961 cacgataata tgcggcgcat gttgcaacga atcacgctgc ataccgaacg gttgaccacc
     4021 gcacaacgtc aaaattttgg tatttggcag aaaacgcgcc agccgacgca attcacctgc
     4081 cacctgatcc gccagttcac gcgtaggaca cagcactaaa gcctgggttt gaaatagcga
     4141 cgcatcaatt tgctgtaaca agccgaggcc aaaagccgcc gttttgccgc tgccggtttt
     4201 cgcctgcacg cgaacatctt ttccggcaag gatcgtcgga agcgcggcgg cctgcaccgg
     4261 cgtcatggtt aaataaccca actcattaag gttcgtgagt tgggcgggag gcaaaacatt
     4321 cagggtagaa aaagcggaca caatttattc tcgtggtcat cgacgcaaag tcagcaggcg
     4381 cgtatcctcg cagatctacg ctcacgatgc gacaatttaa tcggttcttc gtcgggtggt
     4441 gggtcaggca tgggttgcgg gcgagggatc ggatcgggca ctggaacagg atcgccagga
     4501 atcggttcag ggacaggaat ttgcaaataa ataagtgtcg tcatatttcc ctccggtcat
     4561 tgggtggact cttaaagggt agacgctgac aaataacagg caaaaaaaag ccgactcatc
     4621 aaagtcggcg tcgtacgaat caattgtgct atgcagtaat tcaaaaaagg aagtaagaca
     4681 atatggagcg caacgcccat cgcttgacgt tgcattcacc tgcaagagag atattgccct
     4741 gaatgggtag agagtttatt gacttcgctc aaactttgcg gcgtttttgt atacagacag
     4801 ccgggaaaat tgcttttgtt acaaccattt actacgatgc aaccataaag caacaccacc
     4861 aataagaaca actaacagaa tacaaaaaat tgaaaatccg aattgccacc cgccgcctgg
     4921 gatcccacca aggttgacgc caaataaccc tgtcagaaag gtactgggta aaaagaccat
     4981 tgccatcaac gacattgtat aggtacgacg cgctaaattt tcctgcatca cctgagcgat
     5041 ttcatcagcc atcacgccag tccgtgctat acaggcgtcg atttcgtcaa ggccgcgccc
     5101 aaggcgatcg gcaatatcct gcatacgacg gcgttggtca tcgctcatcc acggcaaacg
     5161 ttcactggca agacgagcat aaacatcacg ttgcggtgcc atatagcgac gcatcacaat
     5221 taattgtttg cgcagcagag ccaggaatcc acgcggtgga atttgctgat caaggagatt
     5281 atcttcaagg tcgataattt tatcgtgcag ctgctcgata aattcgctgg aatgatcggt
     5341 caacgcatca cacacatcca ccagccatcc cccgcaatcg gtcggaccgg tgccttcttc
     5401 cagatcgctt accacatcgt ccagcgccag cactttgcgt tgtcgggtcg aaacaattaa
     5461 ccgcccgtcc atatatacac gcatggcgac cagttgatcg gggcgttcat cggtgctgcc
     5521 gtttatacag cgcaatgtaa tcagcgtacc ttcaccgaga cggctgactc ggggccgtgt
     5581 gctctcgccc gccagcgcat cacgtacgtt attgggaagc agcggtgttg tcgccagcca
     5641 ttgggcgcta tcatggtgta cataattaag gtggagccag caggggtgcg cttcatcaat
     5701 cacatctgta ttttccagcg gtttaacgcc gcctctacca tccagcatcc aggcaaatac
     5761 tgcatccggg acattaacgt ccgctccctt aatcgcttcc acagtgcctc catcatcaac
     5821 gcattatttt gtagtctagc cttctggccc tgttacgcaa catctcatca ccccattacc
     5881 ctgaaatgat taataaaatt ctgtctaaat tgaataaaaa aagcaaaatg cttttccata
     5941 tacaaaccgt gtgaagtgtt aaatagcgtc tatcattatc agaattatct gatcatatga
     6001 cgtggctttt ttgcgatcgg atagcaacaa aaattgataa aaataacggg atctcaatga
     6061 ttacgcacaa cttcaatacc ctggacttac tcaccagtcc tgtctggatc gtttcgccct
     6121 ttgaggaaca gttaatttat gccaacagcg cggcgcgact gttgatgcaa gacctcacgt
     6181 ttagtcagct acgaaccggt ccctattccg tctcctcaca aaaagaactg ccgaaatacc
     6241 tctccgatct gcaaaaccaa cacgatatta tcgaaatcct caccgttcag cgcaatgaag
     6301 aggaaacagc attgagctgt cggcttgttt tgcgaaagct gacagaagca gaaccggtga
     6361 ttattttcga aggtatcgaa gcaccggcaa cgctgggttt aaaagccagt cgctcggcaa
     6421 attatcagcg caaaaaacaa ggtttttatg cgcgcttttt tctgactaac tctgcaccaa
     6481 tgttgttgat tgacccctca cgagatggcc aaatcgtcga tgctaacctc gccgcgctca
     6541 atttctatgg ctataaccat gaaacgatgt gccagaaaca tacctgggaa ataaatatgc
     6601 tcgggcgacg cgtcatgcct atcatgcatg aaatttcgca tttacccggt ggccataagc
     6661 ctttgaattt tgttcataaa ctggcggatg gttcgactcg tcatgtgcag acctatgccg
     6721 gtccaattga aatttatggc gacaagctca tgttatgtat tgtgcatgat attactgagc
     6781 aaaaacggct ggaggagcag ctggaacatg ctgcccacca tgatgcgatg accggattac
     6841 tgaatcggcg acagttttat cacattaccg agccaggcca aatgcagcac ctcgccatcg
     6901 ctcaggatta cagcttgttg ctcatcgaca ccgatcgttt taaacacatt aacgatctct
     6961 atgggcattc taaaggtgat gaggtgttat gcgcccttgc ccgcactctc gaaagttgcg
     7021 ctcgcaaagg cgatttggtg tttcgttggg gaggcgaaga gtttgtctta ttgctaccaa
     7081 gaaccccact ggataccgcg ctttcgctgg ctgaaactat ccgcgtaagc gtggcaaaag
     7141 tgagtatttc gggcttacca cgctttactg tcagcattgg tgtggcgcac cacgaaggaa
     7201 atgaaagcat cgatgaactg tttaaacgcg ttgatgatgc tttgtatcga gcgaaaaatg
     7261 atggacgcaa ccgcgtgctg gcggcataag ccacggatgc gtctcgtgat caacgactgc
     7321 gcttagcgtg gcgctcccag ttttcttgct tcgcctgcgc tgttttacgt agtgcgacgt
     7381 aacacgcccc gctgccgcca tgatgcggta gcgcagtgca atatgcctga acatcatcaa
     7441 attcggtcag ccagcgcgcc acatagctgc ggacaatatt ggcatgcgat ttatcatccc
     7501 gtcctttgcc atgaataatc agcacgttac gcaaaccatc cgccagggct tgttgaatga
     7561 aactgaacac cattttgcgg cactcttcca ccggctggcg caaaagattc aggctcgcct
     7621 gttgcggata tttaccactg cgcagcttat ccagcacccc atgttgcaac ccttcccgcc
     7681 gaaactccag cggctgagtt agcgggatga tgtcgagaaa tccggtggtg aggaaattat
     7741 caagctgcag cgtgtcgata cgctgcggcg cacgttggtt acgcgttgga tgccagtgga
     7801 catctgtagc acgtttcagc ggctggacat cttccatggc gtcaagaaac agcgatttgt
     7861 cgtcaaggtt catgtaacct cctatgcacc ttcacacgtc gtgattcaga cgggttaatt
     7921 gttcatactt actgcatcgt tcccttcaca gattgaaaca atactctact tcatacctgt
     7981 gagtatctgc gcaggcgggg cattttatac tagcctgtta gcagtatcga acagtttagc
     8041 ccgagtttaa ttcagcacag gctccgatca ggatgattta ttcatagcag gaaccaacaa
     8101 aaatgcgata aataacagac cactgattag cagaaaggta tccgaaaatg ccatagtttg
     8161 cgcctccctc agcatcaact gactcaataa tttggttgaa gccagtattt ccgatgtctg
     8221 gtcacttcca ctgcgattaa aaaacaaagc acttctggaa atgaagtcat tcaccgtatg
     8281 gggaacagaa accatttttt cacccattct cgaaaaatga aaattagttc gattattgag
     8341 aatagagcca cacagggcaa taccaatcgc gcccccaaga ttacgtgtta aattaaatac
     8401 accagaggct aacttcagcc gttcctttgg aattcccccc agcgttaatg ttacgatggg
     8461 ggccatcgca aactgctgcg aaatccctct aattgcctgt ggaaacagta actcctgcca
     8521 tccccattcg tgggttatag gggtaaaaag atacattgaa aacacaaatc cacccagccc
     8581 cgccatcaac agccatcgca gatttatttt tttacttaac cagaagtaaa agggaacaga
     8641 aaataactgg aaaatacctg ttgtgcaaac ggcaaaacct atctcttcgg catttaatcc
     8701 gcgaacctgc cccaaaaaca caagtattaa atatacagta gagaatatca caacccctcc
     8761 agaaaaagaa aaaaaacatc ccagcgtgaa atttttatcc ttaaaagcat gaagatccat
     8821 tatcggattg gatattgtaa gtgtacgcgc agcaaataaa ataaaactca ccaatgccag
     8881 aactgatgtc agcaaaatag tattatcatc cagccatccc cagcgcgctc cttcttcaag
     8941 ggtatattcc agacatccca gtgtcatggc caacaggata atactcggat aatcagcaac
     9001 tttcagtaaa gagagatcag gtttatcaaa attaaccaga aaaggaatac tcaaaactaa
     9061 atatatgcca ggtaatatat tgatataaaa tagccatcgc cagtcgaggt tatcagtgat
     9121 ccagcccccc agagttggcc ctagcgtcgg tgacagtgag gccagcgcac tgactaccgc
     9181 agcagccgac cccaactctt taccctggta ataaataaac gccgtcgtga aaactaaagg
     9241 gatcatggat gcgccagccg ctccctgtaa agcacgaaac aaaatcatga tctgaatatt
     9301 ccatgcaagt ccacacgcga tactcattaa agtaaatatt cccgcggata gagtaaaaag
     9361 ccaacgagtg gaaaataccc gtgacagcca cccagagagc ggaataacaa taatctcagc
     9421 aattaaatag ctggtctgaa gccaggccat ttcgtcttta cctgcggaca gaccaccacc
     9481 tatttcattt agggatgacg atacaatttg aatatcaagc tgagcaataa ataatcccat
     9541 acacatactg gcgaaagcaa tatgtttacg catagacatc atctctcatc gacactgacg
     9601 gtgacggaca gtcccgggcg aagaagttgc tttaattcct ttgcgtcgtc aaatgcaatc
     9661 cgtaccggga cacgctgaac aattttggtg aaattgccag tggcattttc tatcggaatc
     9721 aagctgaaac tggctcctgt cgctggggaa aggctttcaa tgtgaccgtg aaacacttcc
     9781 cctttgagaa tatcagcacg gatctcagcc ttcattcctg gcttcattcc cgctatttgg
     9841 ttttctttat aatttgcatc aacccacaac tcactgactg gtacaagtga aactaacgat
     9901 gtgccgcctt ccacccaact gcctgtatgg gcgctgcggt ttgcgacaat gccatcaatc
     9961 ggcgcacgaa caagggtata ttccagatcc agtagtgcct gagtcaaagc ggcctgaact
    10021 tcttcgatac tcgcacgtac gttttcttcc tgagcagata aaactgccag ccgttgcttc
    10081 tccaccagga aatcgttcgc ggctttgcgc tcctttgctg cgacctgctg gtagtcaaac
    10141 ctggcgttat caataatttg ctgcgatatt gccgctgatt gtacgagttt ttcataacgt
    10201 tcagtatctc gcaggcgctt ttgctcctca tgttttactg cctgccatgt ttcactggca
    10261 ctttgaatta tcgactgctg catcgtaata gtggcttgaa tgctttcgag attggctttt
    10321 ctacttttta tttttgcctc aagcctggcg acatttgcac gataatcccg atcatcgaga
    10381 cgcaggataa tatcgccttt ttttacgctc tgattatcac gcacctctat agcagaaata
    10441 taaccagaaa ctttacttgc caccgtggta atattaccac caatgtaagc atcatccgtc
    10501 gtctgtataa atcttccttt gcttacccac cagataaaaa agacaacgcc agccaaaata
    10561 ataccgatcg ctactacccc aataagctgt tttttagaaa ttatcatcac tctttacacc
    10621 gtatatatcc ggaaacttgc attcgccata tttaaaattt aacatggctt atttgatgat
    10681 ttctgacaaa atacctactg taaatgacaa tcacactgca acccaaagca tcaccagggc
    10741 atcacattat cggactggat tcggaacatc tgaatggagg cacagttccc tggcataaac
    10801 atctctatgc ccagttgctt tacccggctg aaggagtcgt tcgtgtatgg gctggagaaa
    10861 gcgtctggct agtgcatgcc agcagcgcgc tttggctgcc cccacaaatg ccgcataagt
    10921 ttgttgcgac aggcaacgta cttttaaaaa ccgtactggt ttcagaagcg gaaagtgaaa
    10981 cgctgggaaa agtttgcttc atgacgggta tttcacccct gttaagagaa ttactaattg
    11041 caattaacca acttccaccg tcgcaaagta ccacagataa acaacagtta cgtttttcag
    11101 ccctcgagac actgatactt caggaaataa aaatgggggt gaaaatgtca cttgaactcc
    11161 cttggccaaa tgatgagcgg ctacagcagc tttgtgagaa tttgctaaac aaccaagggt
    11221 atctgccaac actggataat ctagcggata aaattaacgt cagtagccgt acactcatgc
    11281 gcttatttgt taaagagacg ggattaacat ttcgccactg ggttcagcaa atgcacgtta
    11341 tttctgcagt gacgcttctt gatgatggat attcgttgac caaaattgcc catcgtctgg
    11401 gctatgcctc agctgaatcg ttcggcaata tgtttaagcg tcgaacgggc tactcgcccg
    11461 gtaaatttac tcgtcgatta acgatgcatg attatgcaat cacaaggcaa atgatttaac
    11521 ttatattttc gtgaaatctg tcactgaaga aaattggcaa cggatgggta aaaccgttat
    11581 aacactgtca ccggtcacca ggaccccagg ccggatttaa gacgaggatg cactgctgtg
    11641 tgtactgtag agtctggcgg atgtcgacag gctctatttt tttattcatt ttttagctct
    11701 gtaaataacc acattctcgc cgggattggg gaatgagtga cgagatcagt ggtgtctggc
    11761 ggctatcacg ccacagaatc aaataacaga tagcctacgg taaaatttcg ctgactggaa
    11821 ttatcccacc ctcgatccat gtgatggctt gccctcattt cttcaggcag aaaaatcaaa
    11881 aaatcgcttt atgctatcag actgcgacaa accagacgcg aaactgggta cttactattc
    11941 gttagtcttg ccctatccgc ttatcttttt ggtggtatgg ctgctgatgt tgctggcgtg
    12001 gtatcttgtt ggtctgccga taggtccggg gatttaccca cgtttgtctt aagagagaac
    12061 ggatgctgag attacttgaa gaaaaaattg ccacgccact gggtccacta tgggtgattt
    12121 gcgatgagca atttcgcctg cgggcggttg aatgggaaga gtacagcgaa cgcatggtgc
    12181 agctgctgga catccattat cgcaaagaag gctatgagcg catttctgcc gccaacccag
    12241 gtggtttaag cgacaagctt cgtgaatatt ttgccggtaa tcttagcatt attgatacgc
    12301 ttcccactgc cacggggggg acgccatttc agcgcgaagt ctggaaaaca ctacgcacta
    12361 tcccctgcgg gcaggtaatg cattacggtc aactggccga acaattgggt cgccctggcg
    12421 cggcgcgtgc cgttggtgcg gctaacggat cgaatcccat cagcattgtt gtaccttgcc
    12481 atcgggttat tggccgaaac ggcaccatga ccggatatgc aggcggagtt cagcgaaaag
    12541 agtggttatt gcgccacgaa ggttatcttt tgctgtaaac attaaacaat ttgtgccagc
    12601 ttgttcacac ttttatgtaa agttaccctt a
//
LOCUS       DAZGQL010000129        12490 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.129, whole genome shotgun sequence.
ACCESSION   DAZGQL010000129 DAZGQL010000000
VERSION     DAZGQL010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12490
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.129"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(149..451)
                     /locus_tag="ACHKIR_004466"
     CDS             complement(149..451)
                     /locus_tag="ACHKIR_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992112.1"
                     /translation="MSHCTSDGRFVCNDGSLSQSKRFCSGYGVAPAKRQVKSPVTTKL
                     VQKKKSAAVRKVSQPSVAGKNEVVPVTEPRKPTCAPLYMASKPGFTHLPICSGNQY"
     gene            complement(798..1802)
                     /locus_tag="ACHKIR_004467"
     CDS             complement(798..1802)
                     /locus_tag="ACHKIR_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFO9992113.1"
                     /translation="MQNITLIGIDLGKHSFHIHCQDKFGKALLRKKFTRTKLMQFLAT
                     CPPSVVVMEACAGAHFMARRISDFGHETKLISPQFVRPFVKSNKNDFVDAEAICEAAS
                     RPSMRFVQPRTEPLQAMRALHRVRESLIRDKVKTTNQIHGFLLEFGISLPTGDAVIKR
                     LSLVLAEHEIPEYLSRLLVRLHTHYLYLVEQIAELESELSQSINADDTAQRIMTIPGV
                     GPITASLLSSQPGDGKQFSCSRDFAASTGLVPRQYSTGGKSTLLGISKRGDKNLRRLL
                     VQCARSFMMRLERQHGKLAEWVREQLNKKHSNVVACALANKLARIAWVITTQQKEYQA
                     "
     gene            complement(2060..2491)
                     /gene="tssE"
                     /locus_tag="ACHKIR_004468"
     CDS             complement(2060..2491)
                     /gene="tssE"
                     /locus_tag="ACHKIR_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFO9992114.1"
                     /translation="MPRPSLYEILYGNFAGGLELNQVSAEEQVILSVLDNMQRILNTR
                     AGSLKHLPDYGLPDMTTVLQGMPGTAHQLMRVLSDVLLKYEPRIKRVNVTMQEQTQPG
                     ELHYVIDAELKDAGLVRYGTTFIPEGRVLLRHLKQQRYVQT"
     gene            complement(2494..3030)
                     /gene="tssJ"
                     /locus_tag="ACHKIR_004469"
     CDS             complement(2494..3030)
                     /gene="tssJ"
                     /locus_tag="ACHKIR_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFO9992115.1"
                     /translation="MAIIAGKAGYGLIIALFSLSLSGCGLTQRVADGTVSATKSLFYR
                     QIKTLHLDIRAREAINTSAAGIPLSVVVRIYQLKDNRNFDSADYQALFTGDSEILAGD
                     IIAQKDVWMQPGGSVTVDMPLDDAAKFTGVAAMFLEPDRKKNTWRVVLSRDELEPDTP
                     RLIEVSGNTLTLLPVKDK"
     gene            complement(3011..4111)
                     /gene="tssG"
                     /locus_tag="ACHKIR_004470"
     CDS             complement(3011..4111)
                     /gene="tssG"
                     /locus_tag="ACHKIR_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFO9992116.1"
                     /translation="MHPVERKSQSAPARLITRYRKQLPYINFYRFCQLLEQSQPDQPP
                     IGSGWQARQEAVRFCPYTGMGFPASEIKDAVIPEESHLPPTVHVTFMGLYGVTSPLPA
                     HYISDIAQQREGHEAAADFLDIFSHRLITQYYRIWRKYSYPATFEAGGQDKTSQYLLG
                     LARLGIPGCAQNIATPVSRFLALLPLMLLPGRTAEGLTSLVTLLAPGTQARVWHHDRR
                     RIPLKTPLAMRVHHPVSLKSRPVMGDHATDVNGQVLLQLSTQTGSEVQGWLPGGQLYS
                     DLLALLHVYLGSRLDVRLQLCVERSLLPDVRLSCRPAAGSPQLGRTAVMRTQAKITTS
                     AARVMTIRLGRYQRVQEHYQRKEAQENGDYRW"
     gene            complement(4066..5829)
                     /gene="tssF"
                     /locus_tag="ACHKIR_004471"
     CDS             complement(4066..5829)
                     /gene="tssF"
                     /locus_tag="ACHKIR_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFO9992117.1"
                     /translation="MDDLTLRYFDAEMRYLREAGKAFAQAHPDRAAMLDLDKAGTPDP
                     CVERLFEGFAFSMGRLRQKIDDDLPELTESLVSMLWPHYLRTIPSLSVVALTPRLSVM
                     KMAETVPAGLEVTSRPVGPGNTVCRYRTTRAIPLNPLAVEKVVMTTEPDGRSVLKIGF
                     ACSELADWSQVDLHRLSLYLAAEAPVSSTLHLMMTKRLAALYLRLPGNDERIRIDGWF
                     SPGGFAEEDRLWPKGDSAFSGYQLLLEYFTFREKFMFVHLNGLENISLPAGISGFDIE
                     VVLSQSWPADLPVTDDALCLHCVPVINLFTLEADPLIINGLESEYLLRPKRLQDGYTE
                     IYSVDAVAGSGRTGSAEYVPFTSFRHRGGMLRHDAPERYYHTRVKRGVTGMYDTWLIL
                     GGQRWEADRMPERETLSLRITGTNGQLPRRALQSTLLDRCEQVLQTPISVCNLCKPTL
                     PVYPPTEDRFHWRVMSHLGTGFLNMLSSAEVLRGTLALYNWRDDELNHRRLDAILAVQ
                     HHRIQRFEKGFLLRGLDVEVTLDGNGFAGEGDIHLFGEMLNRFLALYADMNQFNQLTL
                     IVQPEGKCIRWKENHNPRLPG"
     gene            complement(5963..7561)
                     /gene="tssA"
                     /locus_tag="ACHKIR_004472"
     CDS             complement(5963..7561)
                     /gene="tssA"
                     /locus_tag="ACHKIR_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HFO9992118.1"
                     /translation="MASIHSLLSACQTTPRDVAEPAQVRIALWDKWLAPVTPDNPAGD
                     DAGYDDDFQQMREEVNKLSGADAGIVSQLAEKLLTTRTKNIRVATWYIWARLRQDGEK
                     GLADGLELLTGLLQRFGEHLHPQRSRARKAALEWLCSARILDSLSLYPEVVKADTLRI
                     AGALWLAEQTFTDEASAPVLNGLYQALENRLMKAGGVDAVVPQEAAAPAPTVTSGSVM
                     ALSAITSGQELLSQARVLAKYLRDQPEGWLAAHRLMKSVRHDTLHQLPPLSADGRTRI
                     APPGPDRRASLKRLYLQQNWLSLLEQCDDMFARGASHLWLDLQWYIHQALLQTGKENY
                     AAIIQYDLKGLLLRLPGLETLAFNDGMPFADDVTLSWIQQQVMECGERWAEEPSVTIT
                     AAPGDNDILSLEPEALQIADNEGTEAALSWLQARPGIQSDRSNWLLRLLMARVAEQTG
                     KNDLALHLLVELDERATRLTLSQWEPELVFEVKARRLKLLRMKSAKTESDRVRLQPDM
                     EHLLAGLIAIDAARAAVLCNSGSS"
     gene            complement(7561..10950)
                     /locus_tag="ACHKIR_004473"
     CDS             complement(7561..10950)
                     /locus_tag="ACHKIR_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImcF-related family protein"
                     /protein_id="HFO9992119.1"
                     /translation="MNKLACLSGRFGRPGIVFIGVAALWWLITRYGAFLGAETRRDQI
                     LLLILLSLGLLFVCYLPVMKKYVQELTYRRRARKEQRLPDDEERLAQTSPRYVTVQDI
                     RHTLRRQHGRFWGRKIRILLITGTASEVELLTPGLTEQFWQEEQGTLLLWGGDPSQPE
                     NADWLAALRRLRYRPADGIVWVTSGLSETLSAPLTEDALDRVSRAVSSCCERLGWRLP
                     LYVWSLQESPDERGRITQPVGCLLPAECSSDKLKAQLQAMLPGLVAQGIQQICCAPRY
                     YFLLSLAERFRRNIDAVVEPLSVLLRPYRQLLLAGIVFSPATVGGERSVRHRWRMDNR
                     WEALPETVQQLPVRLQPSRTGHNWRRSLAVMAAILMMAQGTGMVVSFLANRSLVAEVQ
                     EQIRPAQNQQLSPAERLQALLNLQKSLARLQYREEHGAPWYLRAGMNQNADLLAVVMP
                     LYAQNAHLLLRDAAAAHLEQQLRTFIRLPPDSPQRGKMAKAAYDQLRLYLMLAQPQHM
                     EPARFSRTLMREWPQRDGVSAVFWQANGPTLLAYYASGIITHPQWKLTADEELVSQSR
                     TLLLRHLGTQNSDAMLYQKMLARVAHQFADMRLTDMTGDTDVSRLFFTDEVVPGMFTR
                     QAWEEAVLPSIDTVINERREEMDWVLTDGRQKAPSPVSPEALRQRLTTRYFADFGNAW
                     LNFLNSLHLRKAQTLSDVTEQLTLMADVRQSPLVALMNTLAVQGRTGQPREAVTDSLV
                     KSARNLLSQEKQPVAVPESRLHGPLATTFGPVLALMDNQNNSADMLNLQTYLTRVTQV
                     RLRLQQIAGSSDPQAMMQMLAQTVLQGKSVDLTDTRDYGSLTAAGLGQEWYGFGQTVF
                     VRPMEQAWQQVLTPAAESLNARWRTAVVDGWNNAFSGRYPFKNVSSDASLPLLAKYLN
                     TDTGRIARFLQNNLSGVLHSEGSRWVPDTINTRGLTFNPAFLKAINTLSEIADVAFTT
                     GNAGLHFELRPGTAAGVMQTTLITDNQKLIYVNQMPVWKRFTWPADTEAPGASLSWVS
                     TQAGTRQYADLPGSWGLIRLLEMARRKAAPGVASGWSLSWQAQDGRMLNYTLRTEAGE
                     GPLVLLKLRNFVLPETVFELSGTSAFTGNDEDAGDSVEETD"
     gene            complement(10943..12091)
                     /locus_tag="ACHKIR_004474"
     CDS             complement(10943..12091)
                     /locus_tag="ACHKIR_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein"
                     /protein_id="HFO9992120.1"
                     /translation="MGWSRTKSLSTELPHKPSMMLWFMTGAFMAVVGALLFIIRASEY
                     VKALNDFSIWWLALTPPGGWFFLFCLRHWQWSNQMDEHLFFKKEGEYAQKQWESWAER
                     YLVITASCVYLPDKITVATLCDELPLQYGLVKKIDYLSDSGHKVEASLRVLLREIMDK
                     FCQLPAVLPVNVTLITDQPDSEIRSAFVSAWEVLFPQRVVPDNIEVTPDFSMGWVDER
                     LKQPVLTVDLILVIQLNGGNAYSDGLAALLLTSDDVAQKYNLPHPARLLRPMSLDINK
                     FNDEFTLFLETQTAACRTARVLGDCYHWEKIAAPLMTIGNQYGAGWEPSGRMLLEKWC
                     GIPGPAAPWLLTALAADLVCFGNDSLLTLFSSGEEHFISTVTSGNQNE"
     gene            complement(12095..12361)
                     /locus_tag="ACHKIR_004475"
     CDS             complement(12095..12361)
                     /locus_tag="ACHKIR_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HFO9992121.1"
                     /translation="MSKGFVLLGDNTTHGGKVISASSTMIVDGKKVALVGDMVSCPVS
                     GHGTNPIVEGSPEWISDGRAVVIDGCKCLCGCKVISSAPECAIG"
     gene            complement(12392..>12490)
                     /locus_tag="ACHKIR_004476"
     CDS             complement(12392..>12490)
                     /locus_tag="ACHKIR_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013035836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein"
                     /protein_id="HFO9992122.1"
                     /translation="MCKGVTKHPDGYVYYGDTPDFIKGKIYPYGNW"
BASE COUNT         3137 a         3527 c         2915 g         2911 t
ORIGIN      
        1 ctttattttc cttctcagta actgaatatc tttgattaac gccctccgga aatatttata
       61 cccggaaggc tgtccagctt tatgataccc gccagtacag aattaactgc aggctgagca
      121 cagatttctc tgctatttca gtgtcagatt aatactgatt accggaacaa atgggcagat
      181 gtgtaaatcc aggtttgctg gccatataaa gcggcgcaca ggtgggttta cggggttcag
      241 tgaccggtac aacctcattt ttcccggcca ccgacggttg tgacaccttc cggacagccg
      301 ctgatttttt cttctgaacc agcttcgtgg tgaccggtga tttcacctga cgtttggcgg
      361 gtgccacgcc ataaccggaa caaaagcgct tcgactgact cagacttccg tcattgcaga
      421 caaaacgtcc gtctgacgtg cagtgtgaaa taccaccttt agcacccgag cagggttttc
      481 ttccccgcgc agcatctgtc tgaaccgaaa ataatctatg gtcgcccccg tttttgcaac
      541 actgattttt taattaatgg tgggcttgct taaatctatc cggcgtctct gtaggcaagg
      601 atgcctgcgc cccgatgagt ttcgcacctg ataatcctaa aaagcccctc ggcttcagtg
      661 agccatttta tttattaggt ttttaatcag gtcgatttgc cgttcatgtc atcaataatc
      721 agtcttcgca aaaccagaca ggcaactttg tatcttattg tttacaataa gccaattgag
      781 ttatatcagc atttatatca agcctgatat tctttttgct gagtagtgat tacccaggca
      841 attcgagcca gtttatttgc cagagcgcag gcaaccacat tcgaatgctt cttgttgagt
      901 tgctcccgaa cccattcggc aagttttcca tgctggcgtt caagccgcat catgaatgag
      961 cgggcacact gaaccagtaa ccggcgcaaa tttttatcgc ctcgcttact gatacccaac
     1021 agagtcgatt ttcctcccgt gctatactgc cggggaacga gccctgtgga agccgcaaaa
     1081 tctctgctgc atgaaaactg tttcccgtca ccaggttgcg acgatagtaa gctggcagta
     1141 atcggcccaa caccaggaat tgtcataatg cgctgagcgg tatcatcagc gttgattgac
     1201 tgacttaatt ctgattccag ctctgctatt tgctcaacaa ggtaaaggta gtgggtatgc
     1261 aacctcacca aaaggcggct cagatattcg gggatttcgt gttcagccaa cacgagagac
     1321 aaccgtttta ttacagcatc acctgtcggc aggctgattc caaattccag caagaaaccg
     1381 tggatctggt tggttgtttt caccttatct ctgatgagtg attctcggac ccggtgtaac
     1441 gccctcatgg cctgcagggg ctcagttctt ggctggacaa aacgcattga tggtcgtgaa
     1501 gccgcttcgc atatggcttc tgcatcaaca aaatcattct tattactttt gacaaacggg
     1561 cgaacgaact gtggagaaat tagcttagtt tcatgcccaa aatcagagat acgacgagcc
     1621 ataaagtgtg cacccgcaca ggcttccatt acaacaactg agggcggaca tgtcgccaga
     1681 aactgcatca gtttagtacg ggtaaatttt ttacgcagaa gagccttacc aaacttgtct
     1741 tgacaatgaa tgtggaagga gtgctttccg agatcgatac caataagtgt gatgttctgc
     1801 atgatggtct ctccgttaaa gggattcacc ttaacagcgt actgctgtta aggtgggggt
     1861 gaccatctca ttagtcccac gaacagaaaa cagataattt tcttcattta aaaactcata
     1921 cattgttatt gctgatatgc cagagttatc aggatgatga aacattccgc cagcgttgtc
     1981 ataagccgga ataatgtcac attcacacct gacatatatc catcactgtt tatttctgtt
     2041 ctgccctccc tgctccggct cacgtctgaa cgtaccgctg ctgtttcaga tggcgcaaca
     2101 acaccctgcc ctccggtata aatgttgttc cgtaacgaac cagcccggca tctttaagtt
     2161 ctgcatcaat gacataatgc aactcaccag gctgagtctg ctcctgcatc gtcacgttga
     2221 cacgcttaat acgtggctca tacttcagca gaacatctga aagcacacgc atcagttgat
     2281 gcgccgttcc cggcattccc tgcagaacgg ttgtcatatc cggcagacca taatccggca
     2341 gatgtttaag gcttccggcc cgggtattca gaatacgctg catgttatcg agcaccgaaa
     2401 gaataacctg ctcttccgca ctgacctggt ttaactccag cccgccagcg aaattgccat
     2461 agagaatttc ataaagggaa ggacgcggca tcactattta tccttcaccg gtaacagcgt
     2521 cagggtatta ccggatactt caataagacg gggcgtgtcc ggctcaagct catcccggct
     2581 cagcaccacc cgccaggtat ttttcttccg gtcaggctcc agaaacattg ctgccacacc
     2641 ggtaaatttc gccgcatcat ccagaggcat atccacggtt actgagccac ctggctgcat
     2701 ccatacatct ttctgtgcaa tgatatcgcc tgccagaatt tcactgtccc ccgtaaaaag
     2761 cgcctgataa tccgcgctgt caaaattccg gttgtcttta agctgataaa tccgcaccac
     2821 aaccgacagg ggaataccgg cagcactggt gttaatggcc tccctggcac gaatatcaag
     2881 gtgaagtgtt tttatctgtc ggtaaaacag ggatttcgtg gcagataccg taccgtctgc
     2941 cactctttgc gtcaggccac accctgataa cgacagggaa aacaacgcaa taatcagacc
     3001 gtaaccagcc ttaccagcga taatcgccat tttcctgagc ctccttacgt tgataatgtt
     3061 cctgaacccg ctgataacgc cccagacgga tggtcattac gcgtgccgct gaagtcgtta
     3121 tctttgcctg tgttctcatt acagccgtgc gccccagttg cggactaccg gctgccggtc
     3181 ggcaactgag ccggacatcc ggcaacagtg aacgctcaac acaaagttgc agacgtacat
     3241 ccagacggct tcccagataa acgtgcaaca acgccagcaa atcagaatac agctgcccgc
     3301 ccggtaacca gccctgtacc tcgctgcccg tctgtgttga aagctgtaat aacacctgtc
     3361 cgttcacatc cgtagcatga tcgcccatca ccgggcgact cttcagactg accggatgat
     3421 gaacacgcat ggccagcggt gttttcagcg ggatccgtcg tcggtcatga tgccagacac
     3481 gggcctgtgt tccgggagcc agcagcgtta ccagtgatgt cagcccttca gctgttcttc
     3541 cgggtaacaa catcaggggc aataacgcca gaaaacgcga taccggcgtg gcaatattct
     3601 gtgcacatcc ggggatcccc agtctggcca gaccgagcag atactgggat gttttatcct
     3661 gcccccccgc ctcaaaggtg gccgggtacg aatatttgcg ccagatacgg taatactggg
     3721 tgatcagacg atgactgaat atatcgagaa aatccgctgc ggcttcatgg ccttcccgct
     3781 gctgtgcaat atcactgata tagtgagccg gcaacggaga cgtgaccccg taaagtccca
     3841 taaacgtcac atgtacagtg ggtggcagat gggattcttc aggaattaca gcatctttta
     3901 tttcactcgc aggaaacccc attcccgtgt atggacaaaa gcgaaccgct tcctgacggg
     3961 cctgccagcc actgccaatg ggaggctgat ctggctggct ttgttccagt aactgacaga
     4021 agcgataaaa attaatatac ggaagctgtt tccggtaacg ggtaattaac cgggcaggcg
     4081 cggattgtga ttttctttcc accggatgca ttttccttca ggctgaacaa tcagtgtgag
     4141 ctgattgaac tgattcatat cggcgtacag ggccaggaaa cggttgagca tttcgccaaa
     4201 caggtgaata tctccctctc cggcaaaccc gttgccatca agagttacct ccacatcaag
     4261 cccccggagc agaaaaccct tttcaaaacg ctgaatacga tgatgctgta cagccagaat
     4321 ggcatcaagc cgacggtgat taagttcatc gtcccgccag ttataaagcg ccagcgtgcc
     4381 acgcagcact tccgcactgc tcagcatgtt aagaaatcct gtccccagat gactcatcac
     4441 ccgccagtga aaacgatctt ctgtcggcgg ataaaccggc agcgtgggtt tacaaagatt
     4501 acatacactg atcggggtct gcagtacctg ttcacaccga tcaagcagcg tactctgcaa
     4561 cgcacgacgg ggaagctgac cattggtacc tgtaatacgt aatgaaagcg tttcccgctc
     4621 cggcatccga tccgcctccc accgctgacc gccaaggata agccatgtgt catacatccc
     4681 cgtgacaccc cgcttcaccc gggtatgata ataacgttcg ggtgcatcat ggcgcagcat
     4741 tcccccacga tgacggaagc tggtaaaagg cacgtactct gcactgccgg ttcgcccgga
     4801 gcccgccacc gcatccactg aatatatctc cgtatatcca tcctgcagac gcttcgggcg
     4861 cagcagatat tcgctctcca gcccattgat gatgagtgga tcggcttcaa gcgtaaaaag
     4921 gttaatcacc ggcacacaat gcagacacag ggcatcatct gtcacaggta aatctgccgg
     4981 ccaggactga ctcagcacca cctcaatatc aaatccggaa atacctgccg gcaaagaaat
     5041 attctccagt ccgttcagat gaacaaacat aaatttttcc cgaaaggtga aatattccag
     5101 caataactga tacccactga aagcactgtc acctttcggc cacagccgat cttcttccgc
     5161 aaaaccaccg ggtgaaaacc atccgtcaat gcggatccgc tcatcatttc ccggcagcct
     5221 cagatacagc gcagccagac gctttgtcat catcagatga agggtgctgc tgaccggggc
     5281 ttctgctgcc agatacaggc tcagacgatg caaatcgacc tgagaccagt cagccagttc
     5341 actgcaggca aaacctattt tcagcactga gcgcccgtcc ggctccgtag tcataacgac
     5401 cttctccact gccagtggat tcagtggtat ggcccgggtg gtacggtaac gacagactgt
     5461 atttccgggg ccaacagggc gggaagtcac ttccagtccg gctggtacgg tttcagccat
     5521 tttcatgacg gataaccggg gagtcagtgc caccaccgac agggacggga tagtacgcag
     5581 gtaatgaggc cagagcatac tgaccagact ttcagtaagc tccggcaaat catcatcgat
     5641 tttctggcgc agccgtccca tggaaaaggc gaaaccttca aacaggcgtt ccacgcaggg
     5701 atcaggcgta cccgctttat cgagatccag catcgccgcc cgatcgggat gagcctgtgc
     5761 aaacgccttt ccggcttcac gcagataacg catttcagcg tcaaaataac gcagggttaa
     5821 atcgtccatt atcttctcag aataaagcta actgttccgg agactcgcgc tctgacttca
     5881 gccatatttc aaacaatcgc ccgaactgaa ctgcaggcag gatttcatta atcagattac
     5941 acatgaatat aattcagaca tctcatgaac ttcccgaatt gcacaacaca gcagctctgg
     6001 cagcatcaat ggcgatgagc ccggcaagaa gatgttccat atcaggctgc agacgcaccc
     6061 tgtcactttc agtttttgcg gacttcatac gtaacagttt caggcggcgg gcttttacct
     6121 caaataccag ttcaggttcc cattgtgaca gggtcagccg ggttgcacgt tcatccagct
     6181 ccacaagcag atgcagggca aggtcatttt ttccagtctg ttcggcaaca cgggccatca
     6241 acaggcgcag tagccagttg ctgcggtcag actgaatacc gggacgtgcc tgtaaccagc
     6301 ttaaagcggc ttctgttcct tcattatccg caatctgcag ggcttccggc tcaagagata
     6361 aaatgtcgtt atctccggga gctgccgtta tcgttacaga aggttcttcc gcccagcgtt
     6421 caccacactc catcacctgc tgctgtatcc agctcagcgt cacgtcatcg gcaaacggca
     6481 tgccgtcgtt aaaagccagt gtctcaagcc ccggcaggcg cagaagcaat cctttcaggt
     6541 catactgaat aatggcagca taattttctt ttccggtctg caacagcgcc tgatgaatat
     6601 accactgcag gtccagccac agatgactgg ctccccgggc aaacatatcg tcacactgtt
     6661 caagcagtga caaccagttc tgctgcagat acaaccgttt cagtgacgca cgccggtccg
     6721 gccccggtgg ggcaatacgg gtccgtccat cagcagataa cggcggtaac tgatgcagcg
     6781 tgtcatgccg gacgcttttc atcagtcgat gagccgccag ccagccctca ggctggtcac
     6841 gcagatattt tgccagaaca cgggcctgtg acagcagttc ctgtccggag gtgatggcag
     6901 ataatgccat gacgctgccg gatgttaccg tcggtgccgg cgcggcagcc tcctgaggca
     6961 ccactgcatc aacaccaccg gccttcatca ggcgattttc aagtgcctga tataacccgt
     7021 tcagcaccgg ggcggaagcc tcatccgtaa aggtctgttc tgccagccac agcgccccgg
     7081 caatgcgcag ggtatccgcc ttcaccacct ccggatacag agacagactg tccagtatgc
     7141 gggcgctgca taaccactcc agcgccgcct tcctggcccg tgaccgttgt ggatgcaggt
     7201 gctctccaaa acgctgcaac aaaccggtca gcagctccag accgtcggcc agcccttttt
     7261 caccatcctg ccgcagacgg gcccagatat accaggtcgc cacccggata tttttggttc
     7321 tggtggtcag taatttttct gcaagctggc tgacaattcc ggcatcggct ccggagagtt
     7381 tattgacctc ttcacgcatc tgctgaaaat catcatcgta ccccgcatca tcacctgcgg
     7441 gattatctgg cgtcacggga gcaagccatt tatcccacag cgcaatacgc acctgagccg
     7501 gttcagccac atcccggggt gtcgtctggc atgcactgag gagcgaatga atggaagcca
     7561 tcagtcagtc tcctccacgg aatccccggc atcttcgtca ttgcctgtaa acgctgacgt
     7621 accggagagt tcgaaaaccg tttccggcag aacaaaattg cggagtttca gcaatacaag
     7681 cggcccttcc cccgcttcag ttcgcagtgt gtaattcagc atacgaccgt cctgcgcctg
     7741 ccagctcagg ctccagccac tggcaacgcc cggtgcggct ttccgtcggg ccatctcaag
     7801 cagacgtatc agcccccagc ttcccggcag gtctgcatac tgacgggtac cggcctgagt
     7861 gcttacccag cttaaactgg cgccaggtgc ttccgtatca gccggccagg taaatcgctt
     7921 ccataccggc atctgattaa cataaatcag tttctgatta tcggttatca gcgtcgtctg
     7981 catcacaccg gcagcagttc ccgggcgcag ctcaaaatgc agccccgcgt tacctgtggt
     8041 gaacgccaca tccgctattt cactcagggt attgatggct ttcagaaacg cagggttgaa
     8101 tgtcagtccc cgggtattaa tggtatcagg tacccagcga ctgccttcac tgtgcaacac
     8161 accactcagg ttattctgca ggaaacgggc aatacgtccc gtgtccgtat tcaggtattt
     8221 tgccagcagg ggaagcgaag catcgctgct gacattttta aacggataac gcccggaaaa
     8281 tgcattattc cagccatcca ccaccgcagt acgccaccgg gcattcagac tttctgccgc
     8341 gggcgtaaga acctgctgcc acgcctgctc catcgggcga acaaagaccg tctgtccgaa
     8401 gccataccac tcctgtccga gtccggcagc ggtcaggcta ccgtaatccc gggtatcggt
     8461 taaatcaacc gatttcccct gcaatactgt ctgtgccagc atctgcatca tggcctgcgg
     8521 gtcagaagag ccggcaatct gttgcagacg cagacgaacc tgtgtcaccc gggtcagata
     8581 tgtctgcaga ttcagcatat ccgcactgtt gttctgatta tccatcagtg caagcaccgg
     8641 cccgaaagtc gtcgccagag gaccgtgaag ccggctttcc ggtactgcca ctggctgttt
     8701 ttcctgtgaa agcaggtttc tggcagattt aaccagtgaa tcagtaaccg cttcccgggg
     8761 ctggccagta cgcccctgaa ccgccagcgt attcatcaga gcaaccaggg gggactggcg
     8821 cacatcagcc atcagcgtca gttgctctgt cacatcagac agcgtctgag ctttacgcaa
     8881 gtgcagactg ttcagaaaat tcagccaggc attaccaaaa tcagcaaaat agcgcgttgt
     8941 caggcgctgt ctgagtgctt ccggcgatac cggtgacggt gccttctgcc tgccatccgt
     9001 cagtacccag tccatttctt cacgacgctc attaatcacg gtgtctatcg acggcaatac
     9061 ggcctcttcc caggcctggc gggtaaacat accgggaacc acttcatccg taaaaaagag
     9121 gcgggagaca tcagtatcac cggtcatatc cgtcagacgc atatccgcaa actgatgtgc
     9181 cacccgtgcc agcattttct gatacagcat ggcatcgctg ttctgtgttc ccagatggcg
     9241 aagcagcaga gttctgctct ggctgaccag ctcttcatca gccgtcagct tccactgagg
     9301 atgagtaata atcccggagg cataatacgc cagcaacgtc gggccattag cctgccagaa
     9361 aacggcactc acaccgtcac gctgtggcca ttcccgcatg agcgtacgac tgaacctggc
     9421 gggctccata tgttgcggtt gcgccagcat cagatacagt ctgagctggt catatgcggc
     9481 ttttgccatt ttcccccgct gcgggctgtc cggaggaaga cgaataaatg tcctgagctg
     9541 ctgctcaaga tgagcagcag cggcatcacg cagtaaaaga tgtgcatttt gtgcataaag
     9601 gggcattacg accgcgagca ggtctgcatt ctgattcata ccggcccgca ggtaccatgg
     9661 cgccccgtgc tcctcccggt actgaaggcg ggccagggat ttctgcagat tcagcaacgc
     9721 ctgcagacgt tctgcaggag acagttgctg gttctgagcc ggacgaatct gttcctgtac
     9781 ttcagcaacc agactccggt ttgccagaaa ggaaaccacc attcctgttc cctgagccat
     9841 catcagtatg gccgccataa cggccagact tctgcgccag ttatgacctg tgcgggaagg
     9901 ctgtagccgg acaggtaatt gctgcactgt ctccggcaac gcctcccagc ggttatccat
     9961 tcgccagcga tgtctgacac ttcgttcacc gccgacagtt gccggactga aaacaatacc
    10021 cgccagcaac aactgacggt atggacggag taatacggat aatggctcca ccacagcatc
    10081 aatatttcgt ctgaatcgct ctgccagcga caacaaaaaa taataccgag gggcacagca
    10141 tatctgctgt atcccctgag caacaagccc ggggagcatg gcctgtagtt gtgctttcag
    10201 tttgtcagaa ctacattctg ctggcagaag gcatcccacg ggctgagtaa tacgccctcg
    10261 ctcatccgga ctctcctgca gtgaccagac atacagcggc agacgccagc caaggcgctc
    10321 acaacatgaa ctgaccgcac gggaaaccct gtcaagggca tcttctgtca gtggtgcgga
    10381 taatgtttca ctcagtccgg aagttaccca gacaatgcca tcagcaggcc ggtaacgcag
    10441 acggcgtaac gcagccagcc agtctgcgtt ttccggctgc gaggggtctc ctccccagag
    10501 aagcaatgtc ccctgctctt cctgccagaa ctgttctgtc aggcccgggg tcagcaactc
    10561 aacctcagag gcagtacctg ttatcaacag aatgcgtatt ttacgtcccc agaatctgcc
    10621 atgctggcgc cgcagagtat gacgaatatc ctgaacagta acatagcgcg gtgacgtctg
    10681 agccagtcgc tcttcatcgt caggtaacct ttgctcctta cgggcccgac gtcgatatgt
    10741 cagttcctga acatattttt tcatcacagg caggtaacag acaaagagca accccagact
    10801 cagcaatatc agcagtagta tttgatcccg gcgtgtttct gcccccagaa atgctccata
    10861 tcgggttatc agccaccaga gcgcagccac accaataaat acaatccccg gtcgtccaaa
    10921 gcgaccagac agacaggcca gtttattcat tctggtttcc tgatgtaaca gtactgataa
    10981 aatgttcttc tccggatgag aataaagtca ggagagaatc attaccaaaa cagaccagat
    11041 cggctgccag agcagtaagc agccacggag cagcaggtcc cgggataccg caccattttt
    11101 ccagtaacat acgtccggac ggttcccatc cggcaccata ctggtttcca atagtcatca
    11161 gcggtgcagc aatcttttcc cagtgataac agtcccccag tacacgagcg gtacgacagg
    11221 cagctgtctg ggtttcaagg aaaagtgtga attcatcatt aaacttgttg atatccagcg
    11281 acatcgggcg tagcagacgg gctggatgcg gcaggttata tttttgcgca acatcatcgc
    11341 tggtcaacaa aagtgcagcc agtccgtcag agtatgcatt tcccccatta agctgtatta
    11401 ccagtatcaa atccacagtc agcactggtt gtttcagacg ctcatcaacc caccccatgg
    11461 aaaagtcggg agtaacctcg atattgtcag gaacaacacg ttggggaaat aacacttccc
    11521 atgcagaaac aaaagcagat ctgatttcag aatcaggctg atcggttatc agcgtcacat
    11581 ttacagggag aacggcaggt aactgacaaa atttatccat tatctcccta agcaagacac
    11641 gtaaagaggc ttcaacctta tgcccactgt cagaaagata atctattttc tttacaaggc
    11701 cgtactgcag aggcaattca tcacaaagag tagctaccgt tattttatca ggcaaataaa
    11761 cacaactggc agtaataaca agataccttt cggcccagga ttcccactgt ttctgagcat
    11821 actccccctc ttttttaaag aacaaatgtt catccatctg gttgctccac tgccagtgtc
    11881 gcaggcaaaa caggaaaaac cagccccccg gaggagtcaa tgcaagccac cagatactga
    11941 aatcatttaa tgctttcaca tattctgatg cccggataat aaacaacaga gcaccaacaa
    12001 cagccataaa tgcccctgtc ataaaccaca acatcattga tggtttatga ggtaattctg
    12061 ttgataatga ttttgttctg ctccatccca tgggttatcc tattgcacat tcaggtgcac
    12121 tggatataac cttacaccca cacagacatt tacatccatc aatcacaaca gctcttccat
    12181 cagatatcca ttcaggagag ccctccacaa taggattagt accatgtccg gaaacaggac
    12241 aactaaccat atctccgaca agagctactt ttttaccatc gacaatcatg gttgaagatg
    12301 ccgaaataac tttaccacca tgtgtggtat tatcaccaag caatacaaaa cctttagaca
    12361 taaaactctc aaaaataaat tttactttga gtcaccagtt accgtagggg taaattttcc
    12421 ctttgataaa atctggtgta tcaccgtaat aaacatatcc atctggatgt ttagtaaccc
    12481 ccttacacat
//
LOCUS       DAZGQL010000130        12315 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.130, whole genome shotgun sequence.
ACCESSION   DAZGQL010000130 DAZGQL010000000
VERSION     DAZGQL010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12315
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.130"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..2090
                     /locus_tag="ACHKIR_004477"
     CDS             <1..2090
                     /locus_tag="ACHKIR_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFO9992123.1"
                     /translation="KDREIISTFHAEKVINDLLKNDYKISLSPDTTYRELNKAAQRSI
                     TAPDRIGEGKTWVYQRDTMVERGDNRGVHQYGPAEHFTHIISDKPSPKDKYVAYAINI
                     PDYELAADVYNINVTSPSGQQETFKILINPEHLRQTLERKSLTAVQKSQCEIITPKKP
                     GEAILHAFNATYQQIRENMSEFARSHYGYIQIPPVTTFRADGPETPEEEKGYWFHAYQ
                     PEDLCTIHNPMGDLQDFIALVKDAKKFGIDIIPDYTFNFMGIGGSGKNDLDYPSADIR
                     AKISKDIESGIPGYWQGQVLIPFTIDPVTKERKQIHPEDIHLTAKDFETSKDNISKDE
                     WENLHALKEKRLNGMPKTTPKSDQVIMLQNQYVREMRKYGVRGLRYDAAKHSKHEQIE
                     RSITPPLTIYNERLHNTNLFKPIYHEKAVMNYMEYLVTCQLDEEQMSSLLYERDDLSA
                     IDFSLLMKTIKAFSFGGDLQTLASKPGSTISSIPSKRRILININHDFPNNGNLFNDFL
                     FNHQQDEQLAMAYMAALPFSRPLVYWDGQVLKSTTEIKNYDGSTRVGGEAWLNKGCST
                     YQQLYNEFHALYIDKAGIWSAFEGVFATKNVLAFSRGDSVNINHSPHDGLVIINKGNE
                     EVEGTWPNKLQPGIYKNMGSNSVNIIINNTRKIIPPGKAFMLRGGTLNINIPGRSALL
                     LGKTGEPPNYLYL"
     gene            2427..3404
                     /gene="glaH"
                     /locus_tag="ACHKIR_004478"
     CDS             2427..3404
                     /gene="glaH"
                     /locus_tag="ACHKIR_004478"
                     /EC_number="1.14.11.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050498 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, with 2-oxoglutarate as one donor, and
                     the other dehydrogenated [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutarate dioxygenase GlaH"
                     /protein_id="HFO9992124.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGV
                     DDVKQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATNHYQTHQ"
     gene            3424..4692
                     /gene="lhgO"
                     /locus_tag="ACHKIR_004479"
     CDS             3424..4692
                     /gene="lhgO"
                     /locus_tag="ACHKIR_004479"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HFO9992125.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALQEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSELEMERM
                     RALWERTAANGIEREWLNAEELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            4715..6163
                     /gene="gabD"
                     /locus_tag="ACHKIR_004480"
     CDS             4715..6163
                     /gene="gabD"
                     /locus_tag="ACHKIR_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HFO9992126.1"
                     /translation="MKPNDSNLFRQQALINGEWLDANNGEVIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPAWRALTAKERATILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGVPAGVFNVVTGSAGAVGN
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALASKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLENGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKADERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            6177..7457
                     /gene="gabT"
                     /locus_tag="ACHKIR_004481"
     CDS             6177..7457
                     /gene="gabT"
                     /locus_tag="ACHKIR_004481"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HFO9992127.1"
                     /translation="MSSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYI
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAT
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANDLGHKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHSKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFDEAKQ"
     gene            7695..9095
                     /gene="gabP"
                     /locus_tag="ACHKIR_004482"
     CDS             7695..9095
                     /gene="gabP"
                     /locus_tag="ACHKIR_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015185 - gamma-aminobutyric acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HFO9992128.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            9116..9778
                     /gene="csiR"
                     /locus_tag="ACHKIR_004483"
     CDS             9116..9778
                     /gene="csiR"
                     /locus_tag="ACHKIR_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HFO9992129.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKRDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(9779..10228)
                     /gene="kbp"
                     /locus_tag="ACHKIR_004484"
     CDS             complement(9779..10228)
                     /gene="kbp"
                     /locus_tag="ACHKIR_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HFO9992130.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLNKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPAAASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(10312..10470)
                     /gene="yqaE"
                     /locus_tag="ACHKIR_004485"
     CDS             complement(10312..10470)
                     /gene="yqaE"
                     /locus_tag="ACHKIR_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HFO9992131.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            10653..10952
                     /gene="ygaV"
                     /locus_tag="ACHKIR_004486"
     CDS             10653..10952
                     /gene="ygaV"
                     /locus_tag="ACHKIR_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFO9992132.1"
                     /translation="MTELAQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            10962..11486
                     /gene="ygaP"
                     /locus_tag="ACHKIR_004487"
     CDS             10962..11486
                     /gene="ygaP"
                     /locus_tag="ACHKIR_004487"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HFO9992133.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPAKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKKAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVILGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(11533..11937)
                     /gene="stpA"
                     /locus_tag="ACHKIR_004488"
     CDS             complement(11533..11937)
                     /gene="stpA"
                     /locus_tag="ACHKIR_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HFO9992134.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFIDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
BASE COUNT         3117 a         3022 c         3142 g         3034 t
ORIGIN      
        1 taaaagacag agaaattata tcaacttttc acgcagaaaa agtcattaat gatctgttaa
       61 aaaacgatta taaaatttcc ctttctccag atacaactta ccgagagctg aataaagctg
      121 cacagcgtag cattacagcg ccagacagga taggagaagg gaaaacatgg gtttatcaac
      181 gagatacaat ggttgaaaga ggtgataaca gaggcgttca tcagtatggt ccagctgaac
      241 atttcactca cattatatct gacaaaccat ccccaaaaga taagtatgtt gcatatgcta
      301 ttaacattcc tgactatgag ctagcagccg atgtatataa tattaacgtg acgtcacctt
      361 ccggacagca agaaacattt aaaatactaa tcaatccaga acatctacgg caaacacttg
      421 agcgtaaatc tcttactgct gttcagaaat cacaatgtga aatcatcacc cccaaaaaac
      481 ctggcgaagc gattcttcat gcttttaatg ccacctacca gcaaatcagg gaaaatatgt
      541 ctgagtttgc acgttcccat tatgggtata tacaaattcc tccagtgaca actttccgcg
      601 ccgacggacc agaaactccc gaagaagaaa aaggttactg gtttcacgct tatcaacccg
      661 aagatctttg taccatccat aatccaatgg gagatttgca ggattttatc gcattggtta
      721 aagatgctaa aaaatttggt atcgatatca ttcctgatta tacctttaac tttatgggaa
      781 ttgggggtag tggtaaaaat gacctagatt atccctctgc tgatatacga gcgaagatca
      841 gtaaagatat agaaagtggt atccctggct attggcaagg tcaggttttg attccattta
      901 ctatagatcc agtaacaaaa gaacgtaaac aaatccatcc agaagatata catctcactg
      961 caaaagactt tgaaacaagt aaagataaca tctctaagga tgaatgggaa aacctccatg
     1021 cattaaaaga aaagcgttta aatggaatgc ctaaaacaac ccccaaaagt gaccaggtta
     1081 ttatgttgca aaatcaatac gttcgtgaaa tgcgaaaata tggcgtacga ggtttacgtt
     1141 acgatgcagc aaaacactca aaacatgaac aaatagaaag atcaataact ccaccgctta
     1201 caatttataa tgagcgatta cacaatacta acttatttaa gccaatatat catgaaaaag
     1261 ccgttatgaa ttacatggaa tacctggtaa cttgtcagtt ggatgaagaa caaatgtcat
     1321 cgctgcttta tgaaagagat gatttaagcg ccattgattt ttcattgctc atgaagacga
     1381 taaaagcctt ttcatttggt ggcgatctcc aaacccttgc atcaaaaccg ggttcaacaa
     1441 tctcaagcat cccgtcaaaa agacggatat tgattaacat taaccacgat tttcctaaca
     1501 atggcaatct tttcaatgac tttctattta accatcaaca agatgaacaa ttagcaatgg
     1561 catatatggc cgctctcccg ttcagcaggc ctttagttta ctgggatggc caagtattaa
     1621 aatcaacgac tgaaattaaa aattatgatg ggtcgacgcg tgtcggcggt gaggcgtggc
     1681 ttaataaagg ttgctctacc tatcagcagc tctacaatga attccacgca ttatatatag
     1741 ataaagcagg aatatggagc gcatttgagg gtgtatttgc aactaaaaac gttctggcct
     1801 ttagtcgtgg ggattctgtg aacattaatc actctcctca tgatggacta gttataataa
     1861 ataaaggaaa cgaagaagtt gaaggtacct ggcctaacaa attgcaacct ggaatatata
     1921 aaaacatggg gagtaatagc gttaacatta ttattaataa tacccgaaaa attatccccc
     1981 ctggtaaagc atttatgctt agaggcggaa ctctaaatat caatattcct gggcgtagcg
     2041 ctcttctttt agggaaaact ggagaaccgc cgaactatct ctatttgtaa tttaatgtta
     2101 tatctgcccc gctaaaacgg ggcagataat atgtttagtt tactaacggt cattttgcag
     2161 tgaagccatt taccgttttt tatcgaccag ataatctgtt ctctgatggt aatccacccc
     2221 taacctgttg ctttagttat tcatttcctg tctcactttg ccttaataac ctacgtcaaa
     2281 tgttaccaat ttgttgtttt tgatcgcaat aagagaacaa tatgtaactt ttatgcgcat
     2341 ttttcagaaa tgtagatatt tttagattat ggctacgaaa tgagcatcgc catgtcaccc
     2401 tacatctcat aagaggatcg cttctgatga atgcactgac cgccgtacaa aataacgctg
     2461 tcgattcagg ccaggactat agcggattca ctctcatccc gtcggcgcaa tctccgcgtc
     2521 tgctggaact caccttcacc gaacagacga ccaaacagtt tctcgaacag gttgccgaat
     2581 ggcccgtgca ggcgctggag tacaaatctt ttctgcgttt tcgggtaggc aaaattcttg
     2641 acgatctgtg tgcgaaccag ctgcaaccat tgctgttgaa gaccctgcta aaccgtgctg
     2701 aaggtgcgct gttgatcaat gcggtgggtg tcgatgatgt gaagcaagcg gatgagatgg
     2761 tgaagctggc aacggcggta gcgcatctga ttggtcgctc aaatttcgat gcgatgagcg
     2821 gtcagtatta cgcgcgattc gtggtgaaaa atgttgataa ctcagacagc tatctgcgtc
     2881 agccgcaccg cgtaatggag ctgcacaacg acggcaccta cgtcgaagag atcaccgact
     2941 atgtgctgat gatgaaaatc gacgagcaga acatgcaggg tggaaattcg ttgctgctgc
     3001 atctcgatga ctgggaacat ctggaccact atttccgcca cccgctggcg cgtcgcccga
     3061 tgcgctttgc cgcgcctccg agcaaaaacg tcagcaaaga tgttttccat ccagtgttcg
     3121 acgttgatca acagggtcgc ccggtgatgc gttatatcga ccagttcgtc cagccaaaag
     3181 acttcgaaga aggcgtgtgg ctgagtgagc tttccgatgc cattgaaacc agcaaaggca
     3241 ttctttctgt gcccgttccc gttggcaaat tcctgttgat taacaatcta ttctggctgc
     3301 acggtcgcga ccgctttact ccgcacccgg atctgcgccg tgaactgatg cgtcagcgtg
     3361 gctatttcgc ttacgccact aaccactacc agacgcatca gtaagcgcaa aggaattgag
     3421 cggatgtatg attttgtgat tattggcggc ggcatcatcg gcatgtcgac cgccatgcaa
     3481 ctgattgatg tttatccgga tgcccgcatt gcgttgcagg aaaaagagtc cggcccggcc
     3541 tgtcaccaga cgggccacaa cagtggcgtg atccatgccg gggtctatta cacgcccggt
     3601 agcctgaagg cacagttttg cctggcggga aaccgcgcca ccaaagcctt ttgcgatcaa
     3661 aacggcattc gctatgacaa ctgcggcaag atgctggtcg ccacctctga actcgaaatg
     3721 gaacggatgc gcgcgttgtg ggaacgcacg gcagcgaacg gtatcgagcg cgagtggtta
     3781 aacgccgagg aactgcgcga gcgcgaaccg aatatcaccg ggctcggcgg catttttgtg
     3841 ccgtccagcg gcattgtcag ctaccgcgaa gtgacggcgg cgatggcaaa aatcttccag
     3901 gccagaggcg gcgagattat ttataacgcc gaagtcagcg ccctcagtga gcataaaaac
     3961 ggcgtggtga tacgtacccg tcagggcggt gaatatgaag catcgacgct gattagctgt
     4021 tccgggctga tggctgaccg gctggtgaaa atgctcggcc tcgaaccggg ctttattatc
     4081 tgcccgttcc gtggcgagta tttccgcctt gcgccggagc ataaccagat tgttaaccac
     4141 ctgatttacc ccattcccga ccccgcgatg ccatttttgg gcgtgcatct cacccgcatg
     4201 atcgatggca gcgtgaccgt cgggccaaac gcggtgctgg ctttcaaacg cgaaggctat
     4261 cgtaagcgcg acttctcatt tagcgacacg ctggagattt tgggctcgtc ggggattcgc
     4321 cgggtgctgc aaaaccatct acgctcagga ctgggcgaga tgaaaaactc gctgtgcaaa
     4381 agcggctatc tgcggctggt gcaaaagtat tgtccccggc tttcgttaag cgatctccag
     4441 ccctggcccg ctggtgtgcg ggcgcaggcg gtatcgccgg acggcaagct gattgacgat
     4501 tttctgtttg tcaccacccc gcgcacgatc cacacctgca atgcgccctc cccggcagcg
     4561 acatcagcaa ttcctattgg tgcgcatatt gtcagcaagg tacaaacgct gttggcaagc
     4621 cagagtaacc ccggacgcac gctgcgagcg gcacgtagtg tggatgcctt acacgccgca
     4681 tttaatcaat aacctttgaa aacaggacgt aacaatgaaa cctaacgaca gtaacttatt
     4741 ccgccagcag gcgttgatta acggggaatg gctggacgcc aacaatggcg aggtcatcga
     4801 cgtcaccaat ccggcgaacg gcgacaagct gggtagcgta cccaaaatgg gcgctgatga
     4861 aacccgcgcc gctatcgacg ccgccaaccg cgctctgccc gcctggcgtg cgctcaccgc
     4921 caaagaacgc gccaccattc tgcgcaactg gttcaatttg atgatggagc atcaggacga
     4981 tttagcgcgc ctgatgaccc tagaacaggg caaaccgctg gccgaagcga aaggcgaaat
     5041 tagctacgcc gcctccttta ttgagtggtt tgccgaagaa ggcaaacgca tttatggcga
     5101 caccattcct ggtcatcagg ccgataaacg cctgattgtt atcaagcagc cgattggcgt
     5161 caccgcggct atcacgccgt ggaacttccc ggcggcgatg attacccgca aagccggtcc
     5221 ggcgctggcg gcaggctgca ccatggtgct gaagcccgcc agtcagacgc cgttctctgc
     5281 gctggcgctg gcggagctgg cgattcgcgc aggcgttccg gctggcgtat ttaacgtggt
     5341 caccggttcg gcgggcgcgg tgggtaacga actgaccagc aatccgctgg tgcgcaagct
     5401 gtcgtttacc ggctcgaccg aaattggccg ccaattaatg gaacagtgcg cgaaagacat
     5461 caagaaagtg tcgctggaac tgggcggcaa cgcgccgttt atcgtctttg acgatgccga
     5521 cctcgacaaa gccgtggaag gcgcactggc ctcgaaattc cgcaacgccg ggcaaacctg
     5581 cgtctgcgcc aaccgtttat acgtgcagga cggcgtgtat gaccgctttg ccgaaaaatt
     5641 acagcaggcg gtgagcaaac tgcacatcgg cgacgggctg gagaatggcg tcaccatcgg
     5701 gccgctgatt gatgaaaaag cggtagcaaa agtggaagag catattgccg atgcgctgga
     5761 gaaaggtgcg cgtgtggttt gcggcggtaa agcggatgaa cgcggcggca acttcttcca
     5821 gccgaccatt ctggtggacg ttccggccaa cgccaaagtg tcgaaagaag agacgttcgg
     5881 cccccttgcc ccgctattcc gctttaaaga tgaagccgat gtgattgcgc aggccaatga
     5941 caccgagttt ggcctggccg cctatttcta cgcccgtgat ttaagccgcg tcttccgcgt
     6001 gggcgaggcg ctggagtacg gcatcgtcgg cattaatact ggcattattt ccaatgaagt
     6061 ggccccgttc ggtggcatta aagcctcggg tctgggtcgt gaaggttcga agtatggcat
     6121 cgaagattac ttagaaatca aatatatgtg catcggtctt taactggaga acgcgaatga
     6181 gcagcaataa agagttaatg cagcgccgca gtcaggcgat tccccgtggc gttgggcaaa
     6241 ttcaccccat tttcgctgac cgcgcggaaa actgccgggt gtgggacgtt gaaggccgtg
     6301 agtatattga tttcgcgggc ggcattgcgg tgctcaatac cgggcacctg catccgaaag
     6361 tggttgcggc ggtggaagcg cagttgaaaa agctgtcgca cacctgcttc caggtgctgg
     6421 cttacgaacc gtatctggag ctgtgcgaga ttatgaatca gaaggtgccg ggagatttcg
     6481 ccaaaaaaac gctgctggta acgaccggtt ctgaagcggt ggagaacgcg gtgaaaatcg
     6541 cccgcgccgc caccaaacgc agcgggacca tcgcctttag cggcgcgtat cacgggcgca
     6601 cgcattacac gctggcgctg accggcaagg tgaatccgta ctctgcgggc atgggactga
     6661 tgcctgggca cgtttatcgc gcgctttatc cttgcccgct gcacggcatc agtgaagatg
     6721 acgctatcgc cagcattcac cgaattttta aaaatgacgc tgcgccggaa gatatcgccg
     6781 ccatcgtgat tgagccggtt cagggcgaag gcggtttcta cgccgcgacg ccagccttta
     6841 tgcagcgttt acgcgcgctg tgtgacgagc acgggatcat gctgattgcc gatgaagtgc
     6901 agagcggcgc ggggcgtacc ggcacgctgt ttgcgatgga gcagatgggc gttgcgccgg
     6961 atcttaccac ctttgcgaaa tcgatcgcgg gcggcttccc gctggcgggc gtcaccgggc
     7021 gcgcggaagt gatggatgcc gtcgctccag gcggtctggg cggcacctat gccggtaacc
     7081 cgattgcctg cgtggcagcg ctggaagtgt taaaggtatt cgagcaggaa aatctgctgc
     7141 aaaaagccaa cgacctgggg cataagctga aagacggatt gctggcgata gccgaaaaac
     7201 acccggagat cggcgatgta cgcgggctgg gagcgatgat tgccatcgag ctgtttgaag
     7261 atggcgatca cagcaaaccg gacgccaaac tcaccgccga gatcgtggcg cgtgcccgcg
     7321 ataaaggcct gattcttctc tcctgcggcc cgtattacaa cgtgctgcgc atccttgtac
     7381 cgctcaccat tgaagacgct cagatccgtc agggtctgga aatcatcagc cagtgttttg
     7441 atgaggcgaa gcagtagatc cgtacccatg ccggaggcga cgctgcgcgt cttgtccggc
     7501 ctacgcgaat ccaggtcgga taaagcgttt acgctgcatc cgacaatctg tacgtgaaca
     7561 ggaataaatc tatgtcggcc ggataaggcg gagccgctct ccggtaaaaa gaaccaataa
     7621 caattacacg cgtgacccga cgcgggaaat gtcggggcgc tctcccaagt gacacacttt
     7681 cgagaggatt caggatgggg caatcatcgc aaccacatga gttaggcggc gggctgaagt
     7741 cacgccacgt caccatgttg tctattgccg gtgttatcgg cgcaagtctg tttgtcggtt
     7801 ccagcgtcgc catcgccgaa gcgggcccgg cggtattact ggcctatctg ttcgccggat
     7861 tactggtggt tatgattatg cggatgttgg cggaaatggc ggttgccacg cccgataccg
     7921 gttcgttttc cacctatgcc gacaaggcaa ttggccgctg ggcgggttat accatcggct
     7981 ggctgtactg gtggttttgg gtactggtta tcccgctgga agccaacatc gccgccatga
     8041 tcctgcactc gtgggttcca ggcattccca tctggttatt ttccctggtc attaccctcg
     8101 ccttgactgg cagtaattta ttaagcgtta aaaactacgg cgaatttgag ttctggctgg
     8161 cgctgtgcaa agtcatcgct atcctggcct ttattttcct tggtgcagtc gcaattagcg
     8221 gtttttaccc ttatgccgaa gtgagcggga tctcaagatt gtgggatagc ggcggcttta
     8281 tgcccaacgg cttcggcgca gtattaagcg cgatgttgat caccatgttc tcgtttatgg
     8341 gcgcagaaat tgtcaccatt gccgccgcgg aatctgacac gccggaaaaa catattgtcc
     8401 gcgccaccaa ctcggttatc tggcgtattt ctatcttcta tttatgctct atttttgtcg
     8461 tggtggcatt aataccgtgg aatatgccgg ggctgaaagc cgttggatct tatcgctcgg
     8521 ttctggagtt gctcaatatt ccccatgcga aattaatcat ggactgcgtg atattacttt
     8581 ccgtaaccag ttgcctgaac tcggcgctgt ataccgcttc cagaatgctc tactccttaa
     8641 gccgtcgcgg tgatgctccc gcggtaatgg gtaaaatcaa ccgcagtaaa accccgtatg
     8701 tggcggtatt actctcgacc ggtgcggcat ttttaacggt ggtggtgaat tattacgcgc
     8761 ctgcgaaagt gtttaaattt ctgatcgata gctccggcgc tatcgccctg ctggtttatt
     8821 tagtcattgc cgtttcacaa ttgcggatgc gcaaaattct gcgagcagaa ggaagcgaaa
     8881 ttcgcctgcg gatgtggctt tatccgtggc tcacctggct ggtcatcggt tttataacct
     8941 ttgtgttggt agtgatgcta ttccgcccgg cgcaacagtt agaagtgatt tccaccggtt
     9001 tattagcgat agggataatc tgtaccgtgc caattatggc tcgctggaaa aagctggtat
     9061 tgtggcaaaa aacacccgtt cataatacgc gctaataatg atcaggagtc acaccatgac
     9121 cattacgtct ctggatggct atcgctggct gaagaacgat attattcgcg gaaactttca
     9181 accggatgaa aaattacgga tgagtttgct gacatcgcgt tatgcacttg gcgttgggcc
     9241 gttgcgggaa gcgctttcgc aactggtagc agaacgactg gtcacggtgg tgaatcaaaa
     9301 agggtatcgg gtggcgtcta tgtcagagca agagctgctc gatattttcg acgcccgcgc
     9361 caatatggaa gcgatgctgg tcagtctggc gattgcccgc ggtggcgatg aatgggaggc
     9421 agacgttctc gcaaaagcgc atctgctcag taagctggag gcctgtgacg ccagtgagaa
     9481 aatgcttgat gagtgggatc tgcgtcatca ggcgtttcat actgcaattg tggcgggctg
     9541 tggttctcac tatttgctgc aaatgcgtga acggttgttt gatctggcgg cgcgttatcg
     9601 atttatctgg ctgcggcgaa cagtgctttc ggtggaaatg ctggaagata aacgcgatca
     9661 gcaccagacc ctcactgcgg cggtactggc gcgagatacc gcgcgcgcca gtgagttaat
     9721 gcgccagcat ttactgacgc caattcccat tatccagcag gcgatggctg gcaattaact
     9781 actcttcagg aatacgcaac acttgccccg ggtaaatttt atccgggctt ttgagcatcg
     9841 gtttattcgc ttcgaagatt ttattgtaca gattagcgtt accgtagacc tgtttggaaa
     9901 tggcactcag agtgtcgcca gacttaacgg tataaaactg gctggcagcg gctggtgtcg
     9961 ccgttttcac ctgatcatcg acactggcaa taccggaaat attccccacc gcaacaagga
    10021 ttttctcctt cgcctcctga cttaagccgt cacccgtgac cgtcgctttg ccgtcggcga
    10081 tttgaatgtt cactttatcg gcatcaggta ttccggtttt gttcagatgc tcctgcacct
    10141 tcttcgcctg atcgtcttta tcgtgctgac ctgtaaccgc gtcccagagt ttttctccgg
    10201 catctttcac aaaattgaac agacccatag ctacctcatt cgttaacgga aacactcaga
    10261 aagtgtagca gaggaacgcg gcgcgttaac gccgcggggg tgaacacagg tttaatcgcg
    10321 ggtttgcacc cagaacgcgt gaatcaaacc ggggatatac ccaagcagtg tcagcagaat
    10381 attaataatg aacgcccaac cgaacccttt accgagcagc acgccgagcg gcggcagaat
    10441 gatggtgatg acgattctcc agaaacccat atgtactccc tataagaaaa ttactcattg
    10501 tttaaaaaga gattttatct cttaagcgta gtaattttag cggagcctgc cagtttcagc
    10561 ttacgctttt actctcattt acgctgctga ggctggtata tgttgcattt ttagtttaga
    10621 gtttacttaa tttagaaaat acttaaatat ctatgactga actcgcgcaa ttacaggcta
    10681 gtgccgaaca ggcagcggcc ttattgaaag caatgagcca ccctaaacgg ttgctgattc
    10741 tgtgcatgct tagcggttcc cccggcacca gtgcgggaga gctgacgcgc attaccggac
    10801 tgagtgcctc tgcaacatca cagcatctcg ctcgtatgcg ggacgaaggg cttatcgaca
    10861 gccaacggga tgcccaacgc attctatatt ccattaaaaa tgaggcggta aatgccatta
    10921 tcgccaccct gaaaaatgtc tattgtccgt aaggagtcac tatggctttg acaaccattt
    10981 cgccgcatga tgcacaagaa ttaatcgcac gcggcgcaaa gttaatcgat attcgtgacg
    11041 ctgatgaata tcttcgtgaa catattcctg aagcagatct ggctccatta tccgtgctgg
    11101 aacagtcagg tcttccggct aaattacgtc gcgagcaaat aatattccac tgccaggctg
    11161 gtaaacgcac cagtaataac gccgataaat tagcagcgat tgccgccccc gcagaaatct
    11221 ttttactcga agatgggatt gatggctgga aaaaagcagg attaccagta gcggtaaata
    11281 aatctcaacc cttgccgtta atgcgccagg tgcagatcgc tgcgggtggt ttaatattaa
    11341 tcggcgttat actaggttat accgtaaata gcggtttctt cttattaagc ggctttgttg
    11401 gtgccgggtt actgtttgca ggaattagcg gtttttgtgg gatggcaagg ttgttagata
    11461 agatgccgtg gaaccaacga gcttgagaag cgacgccgga cgcgccatag cagcaacatc
    11521 cggcctcagt aattagatca gaaaatcgtc gagagattta ccttctgcca gcgcctgagc
    11581 gattggcttc ggtgtacggc cctgaccggt ccaggtttta gtttcaccgt taacatcgat
    11641 gaatttatat ttcgccggac gcggctggcg ttttttacca gcgcgtggtg ccgcagcaga
    11701 gctattaccc aataactctt ccgggttaat tccgtcagct ttcatcagct ccagccaggt
    11761 gctaattttt tcctggcgct ctgccagttc acgctgctgt tgttcttctt cttcacgtct
    11821 ttctttagtg acaaccctga atttttcgag catttcttca agaacgtcaa tggagaattc
    11881 gcgagccatc gcacggaggg tgcgaatgtt atttaaactt tgtaacatta cggacataat
    11941 aaagaaaacc ttttaacgcc aaaacaaaaa agtatttcgt cgcgtagtgt aagcgatcac
    12001 ttattatatt tccacaaaat ttaatctatt tacagtcctg cacgagatta tttcagctgt
    12061 tattttctga aaccctcaga ataatttcaa gattatatat tagccatttt gatgagtaat
    12121 ttaggttatt ggtgaacaga gcggcgctct ctgctcgcta attccgcaag cccgacaaaa
    12181 ccaaagtgaa taaaaatgca actcaacaga ataaaagaaa acatctatca ctggtgcttt
    12241 ctgctgtcaa tgtacattat ctggcataaa aaatgctgaa aatgacccga tcactacata
    12301 caccgaattc tctac
//
LOCUS       DAZGQL010000131        11928 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.131, whole genome shotgun sequence.
ACCESSION   DAZGQL010000131 DAZGQL010000000
VERSION     DAZGQL010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11928
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.131"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..271)
                     /locus_tag="ACHKIR_004489"
     CDS             complement(<1..271)
                     /locus_tag="ACHKIR_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992135.1"
                     /translation="MIRIIVFLLFFLSGCTSQKEYSNIVRIENRCSSDIDVFIQGYVS
                     GSTLIYQHNIHLPVGDSDVLVYFSTADIDDGFKYEGAFMIYKDSRG"
     gene            complement(264..1670)
                     /locus_tag="ACHKIR_004490"
     CDS             complement(264..1670)
                     /locus_tag="ACHKIR_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HFO9992136.1"
                     /translation="MGTGYFLVRGDKTTCGGKIIEGADDHTIMGIPQARDMDRVTCGR
                     YPGMFIIVGGVPETDIHGRLMAGTLDSQSSCPCKARFIASMMDDTYETDDGESEAEQH
                     AQSAKKDLTSGSDSSSDDVKLDYRIKLSGNKILTPLNIPDYKEMISGGSTKNTEKIDF
                     TITNKGDEAEALSLEVLDGNEVIYSERQTGKYCDKGEHAWQWDGYSNQGILDTTKIKS
                     KSLLVRLIALCGDMMIKVDYPLKNAAKEEEWVDVKVDRNNKVVDITWRLSVKDSGTKG
                     SNPELSPIPYSDLVSLTKNGVEFYWSRNGSRGGGIGGNIVTAIGVFKVNVKAEINITP
                     SMRTFDLISSLDPDMQSSVSLLGFEKIYHNYGGYYNNSKDEIKALLYSNERYKWDSAH
                     EMGHKVMDKYGSESWTHKGTSTRGQSTIPNTPMPSSGEIDIMKYADSYAPDRYKRLVA
                     ADEDVQALIWLSRVSFND"
     gene            complement(1676..3286)
                     /gene="tssA"
                     /locus_tag="ACHKIR_004491"
     CDS             complement(1676..3286)
                     /gene="tssA"
                     /locus_tag="ACHKIR_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001299109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HFO9992137.1"
                     /translation="MSELLQKLTRSCFADRDALDSARTQAALWQTWLLPVTADTPVGE
                     DPGYHDDFLRIRDEMNKLSGADTDLICQLAESLLLTQAKDVRIATYYIWARLHRDGER
                     GLAEGLALLAGLVERFGAQLLPSRPASRKMALEWLAGEKMLDSLARYPEVAKEDFANI
                     VAALNQLTVSFTAWPEDQHSPSLMPLINALESRLAQSGRMNAVVPQNSSGVPAPSSPV
                     DAPQVQTITSGRDLLDQTKVLARYLNEQPQGWLSAHRLMKTLRWDTVHELPPDVDGKT
                     RLAPPRTESRNQLKRLYAQQNWTELLEQADLMFSTGVSHFWLDIQWYLHQALTKAGAP
                     WDRWTAVIRQDLALLLERLPGLENLAWNDGTPFADELTRNWIAQQVMMREDGAWLAGK
                     AAVPTDDATNDVLALEPEALEMADSQGVEAALGWIQTRPGITTARQRLLLRLLMARVA
                     EQYGKNEMALLLLEELDIAAQGLTLTQWEPDLLFEVKARQLKLLRLRAHRYADKALLN
                     RKMEILLGTLVTIDPVRAAVLCDTQHKD"
     gene            complement(3279..6605)
                     /locus_tag="ACHKIR_004492"
     CDS             complement(3279..6605)
                     /locus_tag="ACHKIR_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImcF-related family protein"
                     /protein_id="HFO9992138.1"
                     /translation="MTVAVVVALCTFIHRKPALLGVEPGSSQQMYWMAGVIAAGVVFL
                     IIMGVYHLLNRHAAVQSFELHRQLAAGDDYRAVTTKKADSRHPAMVELAAFLRDRYGP
                     FWRRKIRLLLVTGEPEQAEAIAPGLTGQHWLEGDHTVLIYGGRPSTEPDVALLTALKK
                     LRRSRPLDGIIWPLTEEQSRQTAQLDKGWRELINGGKRLGFQAPLYLWQVCDNGDYQT
                     GRPLQSVGCLLSERCTPEQLAAMLEAQTLPLTEQGMSQLLTDNRHDFLLRLAHTLAER
                     GIAHWQSILKPLLAGGAFSSLRLRGLMFSPPLTAVPEAAPHAWLPSPVWAGVTGDNAR
                     GRTVGFPWLRITLMSAVCVLAIWGAGMTTSFFANRALVQETSIQTARALDIRLPLSEQ
                     LVALHTLQGELERLQYRIREGAPWYQRFGLERNQQLLAAAFPGYAQAANRLVRDVAVD
                     HLQQQLNAFVALPPNSPQRTATGEQRYKQLKALLMTSRPEKADAAFFSTTLMADDLRY
                     AGVPEGVRQSVLPSLLTFWMANLPEHPQWKTSPPPELTGAVRKILLRQIGVRNAENTL
                     YQNVLQQVSRNYADMTLADMTGDTLTESLFSTEQTVPGMFTRQAWEGQVREAIEQVVT
                     ARREEIDWVLSDRQQDTSADISPDMLRNRLTSRYFTDFAGSWLAFLNSIRWKKEDSLS
                     GILDQLTLMADARQSPLIALTDTLAWQAAAGRENRGLSDSLAKSAQELFNGKEKTPQQ
                     SREGDVPVGPLDKTFAPLLRLLGDKAGGGDSQLSLQTYLTRVTRVRLKLQQVTNAPDP
                     QEMTQQLAQTVLQGKTVDLTDTRDYGSLIAASLGEEWSGFGQALFVRPVEQSWRQVLT
                     PAADSLNRQWQRAIVSHWDQDFAGRYPFKASQNDASLPLLAQYLRDDGRINQFIAANL
                     SGVLKREGRYWVADAMNTQGLTVNPDFIRALNRLRDVADTAFASGDAGIHFELRAKPA
                     RDVMKTHLVIDGQELEYFNQKERWQRFNWPDEQWQPGASLSWTSTQAMERILADYRGS
                     WSLIRLLEQAQVTPVDSSTFKVVWKAQDGLPLNYLLRVEQGKGPLALLELKNFRLPGQ
                     VFLTGRSMKDAEEYGEDADE"
     gene            complement(6662..7876)
                     /locus_tag="ACHKIR_004493"
     CDS             complement(6662..7876)
                     /locus_tag="ACHKIR_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992139.1"
                     /translation="MPIRLNTIPDAEPYPAPPARIKWLSALLLMLSVGVAITSLFASD
                     ELAKNGPYFWGLACGVPAFIWSLVGSVRWLVFVTQHIRADAWNQRREEVILQETRRGR
                     RALQILSFSVQTALNGDSVAETTTAFLARHQVLNTYADLRGEDTVRRSVIPVLTNKPV
                     TGRLSEIISRLFIDIRPQLFLLPPDFHINVLLEIDAPLSGASVRTIWQDEWQKAGLPE
                     ARLFSAPEPGLAAVDDWLDNFIQEKAVLLVISVRLEPKNPERTAESATALLLANRLTQ
                     TALTPLALLHRPERITDTEMMASGIAQALDWMPVQPDAISGMWTVELNREQRASLLSS
                     NQPFTQEALMYELDAFLGCSGPAAPWLSVAAATLAAIQSQHPQLTLSGVQNGHYSWAT
                     MVSPFVSPQEAS"
     gene            complement(7982..8371)
                     /locus_tag="ACHKIR_004494"
     CDS             complement(7982..8371)
                     /locus_tag="ACHKIR_004494"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992140.1"
                     /translation="MLINFFFCSNSYSEEKIEIININKPIYIGTNQSDKVLITACNHW
                     VLNSRDIIDIFNLSIKHDDYGEINRMYYWLPCEIRGRLMYDGIEWSFVINAAATAMWM
                     SNDKEIYWGCNKKACDNFFLLPYDGMR"
     gene            complement(8361..9095)
                     /locus_tag="ACHKIR_004495"
     CDS             complement(8361..9095)
                     /locus_tag="ACHKIR_004495"
                     /inference="COORDINATES: protein motif:HMM:NF013661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFO9992141.1"
                     /translation="MSKNVILSNGERENPSPLIYDKHKKHCIFRTKTLLSEEKKEDEK
                     CTVSVVFIDHEGYIRNAGIIKHTVPELEEGRMPKINAIILHRTDSSTINGTLASFSKG
                     IGTHFIVEKDGNIYQAASLFVYTSHVGKIKSRCKEEKTWSSEEKKKTEAFGWNAKKIH
                     DHEKIKQYPYRYPYNMDSVGIEVIALYDKKNKKWESATDSQKKSIEKLVDIIKNIYEL
                     EDRDVYEHDKISYKTEGEGADLYSVN"
     gene            complement(9128..11614)
                     /locus_tag="ACHKIR_004496"
     CDS             complement(9128..11614)
                     /locus_tag="ACHKIR_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HFO9992142.1"
                     /translation="MSIPALNFDHSHHKLKIRGLQSPVDVLTFEGREQLSTPFRYDIQ
                     FTSTDKAITPELVLMQDGAFSLTAPPVQGMPVQAPLRTLYGVITGFKHLSSSQDEARY
                     EVRLEPRMALLARSRQNAIYQNLTVPQIVEKILRERHQMRGQDFVFNLKSEYPAREQV
                     MQYGEDDLTFISRLLSEVGIWFRFATDARLKIEVVEFYDDQSGYERGLTLPLRHPSGL
                     FDGETEAVWGLNTAYSVVEKSVTTRDYNYRTATAEMMTEQHDATGGDNTTYGEAYHYA
                     DNFLQKGDKEAAESGAFYARIRHERYLNEQAILKGQSTSSLLMPGLEIRVQGDDAPAV
                     FRKGVLITGVTASAARDRSYELTFTAIPYSERYGYRPAFIPRPVMAGTLPARVTSTVK
                     NDIYAHIDKDGRYRVNLDFDRDTWKPGYESLWVRQSRPYAGDTYGLHLPLLAGTEVSI
                     AFEEGNPDRPYIAGVKHDSAHTDHVTIQNYKRNVLRTPANNKIRLDDERGKEHIKVST
                     EYGGKSQLNLGHLVDAGKQQRGEGFELRTDMWGAVRAKKGIFISADAQDKAQGQVREM
                     APAMAILDGAQSQMKSLSTDAQTANADPADLSSQIALLQQSVKDLTQAVILLSAPKGV
                     AIASGEHLQLAAAKNVIANAGNNADVGVVKNMFIGVGQALSVFVRKAGIKLFANKGAI
                     SVQAQNDLMELLAKKEIIITSTEDEIKITAKKKITLNGGGSYIRLDACGIEAGTPGEY
                     NVKAGYYGRKPKAKLTPELMAFPVIEGGDYSRCFKFFDDENIPYRNVKYIANFIDGTK
                     KEGVTDDEGCTETFFSDKEEEIEIEILLGV"
     gene            complement(11662..>11928)
                     /locus_tag="ACHKIR_004497"
     CDS             complement(11662..>11928)
                     /locus_tag="ACHKIR_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992143.1"
                     /translation="KGNLIDLVTNSSFFVETIKSAGIKEPVPWDVFPELEPDTFGSLQ
                     GELEFWWESIWSPFWHSLSPDERNDFLVKNNATPQWREFIEFHC"
BASE COUNT         2577 a         3381 c         2919 g         3051 t
ORIGIN      
        1 caccgcggct atctttatat atcatgaatg ccccctcata tttaaaacca tcatcaatat
       61 ctgccgtgga aaaatatact aatacatcac tatcaccaac tggcaagtgt atattatgct
      121 gataaatcag cgtagaaccg gaaacatatc cctgaataaa gacatcgata tctgaggagc
      181 atctattctc aatcctgact atattggaat actctttttg tgaagtacac ccactcaaaa
      241 aaaacaataa aaaaacaatt attctaatca ttaaaactca ccctgctcaa ccagatcaat
      301 gcctgaacat cttcatctgc cgcgaccaat ctcttatatc tatcaggtgc atagctatct
      361 gcatatttca ttatatcaat ctctcctgaa gatggcattg gagtattagg gatggtactt
      421 tgaccccgcg tggatgtccc cttatgtgtc catgattcag aaccatattt atccataact
      481 ttatgtccca tctcatgagc actatcccat ttatatcttt cattactata tagaagagcc
      541 tttatttcat ccttgctgtt attataatag ccaccatagt tatggtatat tttttcaaac
      601 cccaaaagac ttaccgacga ctgcatatct ggatctaacg atgatatcaa atcaaaagtt
      661 cgcatagatg gagtaatatt tatctcagcc tttacattta ctttaaaaac cccaatcgct
      721 gtaacgatat taccaccaat tcccccgccg cgacttccat ttcgtgacca gtaaaactcc
      781 acgccatttt tagtaagact aactaaatcg ctataaggta ttggcgataa ctcagggtta
      841 ctgccttttg taccgctatc cttaacagat aatctccatg ttatatcgac cactttatta
      901 tttctgtcaa cttttacatc aacccactct tcttctttcg cagcattttt caatggataa
      961 tcaactttta tcatcatatc accacataaa gcaatcagtc ttacgagtag tgatttgctt
     1021 tttatcttag ttgtatccag tatgccttga ttactgtagc catcccattg ccaggcatgc
     1081 tctcccttat cacaatattt accggtctgc cgttcactat aaatcacttc attaccatca
     1141 agcacttcca gagaaagcgc ttcagcctca tcgcctttat ttgtgatggt aaaatctatt
     1201 ttttctgtat tcttcgtact accaccgcta atcatttctt tataatcagg tatattcagt
     1261 ggcgtcagta tcttattacc tgataattta attctgtaat ccagttttac atcatcagag
     1321 gatgaatcag aaccagaagt cagatccttt ttcgccgact gcgcatgttg ttccgcttca
     1381 ctttccccgt catccgtctc atacgtatca tccatcattg acgcaataaa ccgtgcctta
     1441 cacgggcaac tgctctgact gtccagcgtc cccgccatca gcctgccgtg aatatctgtt
     1501 tcaggaacgc cgcccacaat aataaacatt cccggatatc tgccgcaggt gacccggtcc
     1561 atatcccgcg cctgcgggat acccatgatc gtatggtcgt ccgcgccttc aataattttg
     1621 ccgccgcagg tggttttgtc ccccctgacc agaaagtagc ctgttcccat tgccatcagt
     1681 ccttatgttg cgtatcacac aataccgctg cccgtaccgg atctattgtc accagcgttc
     1741 ccagcaggat ttccattttt cggttcagca gggctttatc cgcatagcgg tgcgcccgca
     1801 ggcgcagcag tttcagctgt cgggctttta cctcaaacag caggtccggc tcccactgcg
     1861 tcagcgtcag cccctgtgcg gcgatatcca gttcttccag cagcaggagc gccatctcgt
     1921 ttttaccgta ctgttccgcc acccgcgcca tcagcagtcg caataacagg cgctggcgtg
     1981 ccgtggtgat gcccggacgg gtctgtatcc agcccagcgc cgcttccacg ccctgactgt
     2041 ctgccatctc cagcgcttcc ggctccagcg ccagtacgtc gtttgtggcg tcgtctgtcg
     2101 gtacagcggc tttccccgca agccatgcgc cgtcttcccg catcatgacc tgctgcgcta
     2161 tccagttgcg cgtcaactca tcggcgaacg gcgtgccgtc gttccatgcc agattttcca
     2221 gccccggcag gcgttccagt agtaacgcca ggtcctgacg gataaccgct gtccacctgt
     2281 cccacggtgc gcccgcctta gtcagcgcct gatgcagata ccactgaatg tccagccaga
     2341 aatggctgac gccggtgctg aacatcaggt cggcctgctc cagcagttcc gtccagttct
     2401 gctgagcata cagccgctta agctggttgc gtgattctgt gcgcggcggg gccagccggg
     2461 tttttccgtc cacatccggc ggcagttcat gcaccgtgtc ccagcgcagg gttttcatca
     2521 gccggtgtgc tgaaagccag ccctgcggct gttcgttcag ataacgggcc agcactttcg
     2581 tctggtcgag caggtcgcgc ccggaagtga tggtctgtac ctgtggcgca tccaccgggg
     2641 aagacggtgc cggaacgccg ctgctgttct gcggcaccac cgcgttcatc ctacccgact
     2701 gcgccagacg tgattccagc gcgttaatca gcggcatcag ggagggggaa tgctggtctt
     2761 cgggccatgc agtgaaggaa accgtcagtt gattaagagc ggcaacaatg ttcgcaaaat
     2821 cctcttttgc cacttccggg tagcgggcaa ggctgtcgag catcttttcc cctgccagcc
     2881 actccagcgc catttttcgg cttgccgggc gcgagggcag cagttgcgca ccgaagcgtt
     2941 ccaccagtcc ggctaacagc gccagcccct cagccagtcc gcgttcgcca tcgcggtgca
     3001 gtcgcgccca gatatagtaa gtggcgatgc gcacgtcttt tgcctgcgtc agcagcaggg
     3061 attcggcaag ctggcagata aggtccgtat ccgcgccgga aagtttgttc atctcgtcgc
     3121 ggatacgcag gaaatcgtcg tggtagccgg ggtcttcccc gaccggcgta tctgccgtca
     3181 ccggcagcag ccatgtctgc cacagcgccg cctgtgttcg cgccgagtcc agtgcgtccc
     3241 ggtcggcaaa acagctacgg gtcagctttt gcaacagttc actcatcggc gtcttccccg
     3301 tattcttccg catccttcat actcctgccg gtcaggaaca cctgtcccgg caggcggaag
     3361 tttttcagtt ccagcagcgc cagcggccct ttaccctgtt caacccgcag caggtaattc
     3421 agcggcaggc cgtcctgcgc tttccacacc actttaaagg tactgctgtc caccggtgtc
     3481 acctgcgcct gttccagcag gcgaataagg ctccagcttc cccgataatc cgccagtatg
     3541 cgttccatcg cctgcgtact ggtccagctc agcgatgcgc cgggttgcca ctgttcatcc
     3601 ggccagttaa aacgctgcca gcgttccttc tggttgaaat attccagctc ctgcccgtca
     3661 atcaccagat gcgtcttcat cacgtcgcgc gccggttttg cccgcagttc aaaatgtatc
     3721 cctgcatcgc cgctggcaaa ggcggtatcg gccacatcgc gcaggcggtt cagggcgcga
     3781 ataaagtccg gattgactgt cagcccctgc gtgttcatgg cgtcagccac ccagtagcgg
     3841 ccttcacgtt tcagtacgcc ggaaagattg gcggcgataa actggttgat gcgcccgtca
     3901 tcgcgcaggt actgcgccag cagggggagg gaggcatcgt tttgtgaggc tttgaacggg
     3961 tagcgaccag cgaagtcctg atcccagtgg ctgacaatcg cccgctgcca ctggcggttc
     4021 aggctgtctg ccgcaggcgt cagcacctgc cgccatgact gctccaccgg gcggacgaac
     4081 agcgcctgac cgaagccact ccactcttca cccagactgg cggctatcag gctgccatag
     4141 tcgcgggtgt cggtaaggtc aacggttttg ccttgtaaga ccgtctgcgc cagttgctgg
     4201 gtcatctcct gcgggtcagg ggcgttggtc acctgttgca gtttgaggcg cacgcgggtg
     4261 actcgggtga ggtaggtctg caggctcagc tggctgtcgc cgcctcctgc cttatcgcca
     4321 agcaaccgca gcagcggcgc gaaggtttta tccagcggcc cgacaggcac gtcgccttca
     4381 cgggattgct gcggcgtttt ctccttgccg ttaaacagtt cctgtgccga tttcgccagc
     4441 gagtctgaca ggccacggtt ttccctgcca gccgccgcct gccacgcgag ggtgtctgtc
     4501 agcgcaatca gcggtgactg gcgggcatcg gccatcagcg tcaactggtc gagaatgccg
     4561 gagagcgagt cttccttttt ccagcgaatg ctgttgagaa acgccagcca gcttccggca
     4621 aagtcggtaa agtagcgtga agtgagacgg ttacgcagca tatccggcga gatatccgca
     4681 gaggtatcct gctggcggtc gctgagtacc cagtcgattt cctcgcgccg cgccgtcacc
     4741 acctgctcga tggcttccct gacctgtcct tcccacgcct gacgggtaaa catccccggc
     4801 accgtctgct ccgtactgaa aagagattcg gtgagcgtat ccccggtcat gtccgccagc
     4861 gtcatatcgg cgtagttgcg ggacacctgt tgcagtacgt tctggtagag agtgttttcg
     4921 gcattgcgca caccaatctg gcgcagcagg attttgcgca ctgcgccggt cagttccggt
     4981 ggcggcgaag ttttccactg tgggtgttcc ggcagattcg ccatccagaa ggtcagcagt
     5041 gacggcaaca cgctctgacg cacaccttcc ggtacacctg catagcgcag gtcatctgcc
     5101 atcagcgtgg tactgaaaaa ggcagcgtcg gccttttccg ggcgggaagt catcagcaat
     5161 gccttaagct gcttatagcg ctgttcgccg gtggcggtac gctgcggact attgggcggc
     5221 agcgcgacaa aggcattcag ttgctgttgc agatggtcaa cggccacgtc gcgcaccagc
     5281 cggtttgccg cctgcgcata gccgggaaag gcggcggcga gcagttgctg gttacgttca
     5341 aggccaaaac gctgatacca cggcgcaccc tcccggatac ggtactgcag gcgctccagt
     5401 tcaccctgca gggtatgcag cgccaccagt tgttcagaca gcggcagacg gatatcgagt
     5461 gcacgcgccg tctggatgct ggtttcctga acaagtgcgc ggttggcgaa gaacgaggtc
     5521 gtcattccgg ccccccatat cgccagtacg cagacagcag acatcagcgt gatacgcagc
     5581 cacggaaagc ccaccgtgcg accccgcgcg ttatcgccgg tcacgcccgc ccagaccggt
     5641 gacggcagcc acgcatgagg tgcggcttcc ggtacggcgg taagcggcgg gctgaacatc
     5701 aggccacgca ggcgcaggga ggaaaatgcg ccgcctgcca gcagcggttt caggatactt
     5761 tgccagtgag cgataccccg ctctgcaagg gtatgggcca gacgcagcag gaaatcatgg
     5821 cggttatcgg taagcagctg cgacatgccc tgttccgtca gcggcagggt ttgtgcttcc
     5881 agcattgcag ccagttgttc cggggtacag cgttccgaca gcaggcagcc gacgctttgc
     5941 agggggcgtc cggtctgata atcaccgttg tcacagacct gccacaaata gagtggagcc
     6001 tgaaaaccga gtcgcttacc gccgtttatc agttcgcgcc agcctttgtc gagttgcgcc
     6061 gtctggcggc tctgttcttc tgtcagcggc cagatgatgc cgtccagcgg acggctgcgg
     6121 cgcagttttt ttaaggcggt cagtagcgcg acatcaggct ctgtagatgg cctgccgcca
     6181 tatatcagca ccgtgtggtc gccctccagc cagtgttgcc cggtcagccc cggcgcgatg
     6241 gcctctgcct gttcaggctc gccggtcacc agcagcaggc ggattttacg ccgccagaat
     6301 gggccgtagc ggtcgcgcag gaaggcggcg agttccacca ttgccggatg acggctgtcg
     6361 gctttctttg tcgtcaccgc ccgatagtca tctccggcgg caagctgtct gtggagttca
     6421 aagctctgta cggcagcgtg gcggttcagc agatgataaa cccccatgat aatcagaaag
     6481 accacacctg cggcaatcac gccagccatc cagtacattt gctgtgaact acccggttca
     6541 acgcccagca atgccggttt acggtgaata aacgtgcaaa gcgcaaccac aacggccacg
     6601 gtcatgacga tggcaggccc tgaaaccgaa cggggccgga gacggtttaa tcccggaacc
     6661 actatgatgc ctcctgaggg gaaacgaatg gcgacaccat ggtcgcccat gaataatgcc
     6721 cgttctgaac gccgctgagc gtcagttggg ggtgttgtga ttgtattgcc gccagcgttg
     6781 ccgcagcgac ggacagccac ggcgctgccg gtccggagca ccccagaaag gcatccagct
     6841 cgtacatcag ggcttcctga gtaaacggct ggttagatga cagcagcgag gcgcgctgtt
     6901 ccctgttcag ttcgactgtc cacatgccgg atatggcatc tggctgcacc ggcatccagt
     6961 ccagcgcctg tgcaatgcct gacgccatca tctccgtatc tgtgatacgt tcaggacgat
     7021 gcagcagggc cagcggcgtc agcgcggttt gcgtcagtcg gtttgccagc aacagcgcgg
     7081 tggcggattc tgccgtccgt tccgggttct tcggttccag acgcacggaa atcaccagca
     7141 gtacggcctt ttcctgaata aagttgtcca gccagtcgtc cacggcggcc agccccggct
     7201 ccggggcaga aaacagacgg gcttcgggga gtccggcttt ttgccattcg tcctgccaga
     7261 tagttctcac ggaggcccct gaaagcggcg catcaatttc cagtagtacg ttgatatgaa
     7321 aatcaggggg cagcagaaac aattgcggtc tgatatcaat aaaaaggcgg gaaataattt
     7381 cggataaacg cccggtaacc ggtttattgg ttaatacggg aatgacactg cgacgcacgg
     7441 tatcttctcc gcgcaaatca gcgtaagtat tcaacacctg atgacgcgcc agaaatgctg
     7501 ttgtagtttc tgcgacggaa tcaccattca gcgcggtttg tacggaaaaa ctgagtatct
     7561 gcaacgcccg tcgtccccgc cgtgtttcct gcaaaatgac ttcttcgcga cgttgattcc
     7621 aggcatcggc acgaatatgt tgagtgacaa ataccagcca gcgcaccgaa ccgaccagtg
     7681 accagataaa agcaggcacg ccgcaggcca gcccccagaa atatgggccg ttttttgcca
     7741 gttcatcgct ggcgaataat gaggttattg ccacaccaac agacagcatc agtaataagg
     7801 ctgacagcca tttaatgcgg gctggcggtg cgggataggg ttctgcgtcg ggaatggtat
     7861 taagtcggat gggcattgta ataatccgtt attctgaaat atgaatacaa tgaatctcaa
     7921 ggtgcttgaa ctatctgatt aattcacttg tccataatgt ttttgcttta ttaactgtga
     7981 tttacctcat cccatcatat ggtaataaaa agaaattatc gcatgctttt ttattgcatc
     8041 cccagtaaat ctctttatca ttgctcatcc acattgctgt tgcagcagca ttgataacaa
     8101 aactccattc tattccgtca tacattagtc tccctctaat ttcacaaggt aaccaataat
     8161 acattctgtt tatttcgcca taatcatcat gctttatact tagattgaat atgtctataa
     8221 tatctctaga attaagcacc caatgattac acgcagtaat aagcacctta tctgattgat
     8281 tggtacctat atatattggt ttattaatgt ttattatctc tattttttct tcggagtaac
     8341 tatttgaaca aaaaaagaaa ttaattaaca ctatagagat cagcgccttc accttctgtt
     8401 ttataactaa ttttatcatg ttcataaaca tccctatctt ctaactcata aatatttttt
     8461 attatatcta caagtttctc aattgatttt ttttgagagt ctgtagcaga ctcccatttt
     8521 ttattttttt tatcatataa tgcaataacc tcaatgccaa cactgtccat attatatgga
     8581 tatctataag gatattgttt aattttttca tgatcatgta tcttcttagc attccatccg
     8641 aatgcctctg ttttcttttt ttcttcagac gaccatgttt tttcctcctt acatcgagac
     8701 tttatcttcc ccacatggct ggtatataca aataggcttg cagcctggta tatattacca
     8761 tctttttcaa caataaaatg agttccaatg cctttagaaa aactagcaag tgtaccattt
     8821 attgttgaag aatctgttct atgcaatatt attgcattaa ttttaggcat gcgaccttct
     8881 tcaagttcag ggactgtgtg ttttataatt ccagcatttc ttatataccc ctcatgatca
     8941 ataaatacaa ctgatacagt acatttttcg tcttcttttt tctcttcact aagtaatgtc
     9001 tttgtacgaa aaatacagtg ctttttatgc ttgtcataga tcagcggaga aggattttct
     9061 ctttccccat tacttaatat tacatttttg ctcatttttt tctctttaat aattaagggt
     9121 ggtattttta aacaccaagt aaaatctcaa tttcaatttc ttcttcttta tcactaaaaa
     9181 atgtttcggt gcatccctca tcatcagtga caccttcctt ttttgttcca tcaataaaat
     9241 tggctatata cttaacatta cgatatggga tattttcgtc atcaaaaaat ttaaaacagc
     9301 gagaatagtc tccaccctca atcaccggaa acgccatcag ctccggcgtc agcttcgcct
     9361 tcggcttacg cccataatat cccgccttaa cgttatattc tcccggcgtt cccgcttcta
     9421 tcccacaggc atccagccgg atgtagctgc caccgccgtt aagggtgatc tttttcttcg
     9481 ccgtgatttt aatttcatct tcggtactgg taatgatgat ttccttcttc gccaacagtt
     9541 ccatcaagtc gttctgcgcc tgcaccgaga ttgcgccctt attggcaaac agcttgatgc
     9601 ccgctttacg cacaaacacg ctcagcgcct gtcccacgcc gataaacata tttttcacca
     9661 cgccgacatc ggcgttgttg cctgcgttgg ctatcacatt ctttgccgcc gccagttgca
     9721 ggtgctcgcc gctggcgatc gccacgcctt tgggtgcgct tagcaggatc accgcctgcg
     9781 tcagatcttt tacactctgc tgcaacagcg ctatctgtga tgacagatct gccggatcgg
     9841 cattcgccgt ctgcgcatca gtggacagcg acttcatctg tgactgtgcg ccatccagta
     9901 ttgccattgc cggagccatt tcccgcacct gaccctgagc cttgtcctgc gcatcggcgc
     9961 tgataaagat gcctttcttc gcccgcactg cgccccacat atcggtacgc agttcaaagc
    10021 cttcgccgcg ctgctgtttc ccggcatcca ccagatggcc gaggtttagc tggctcttgc
    10081 cgccgtactc ggtactgacc ttgatatgct ctttgccgcg ctcatcgtcg aggcggattt
    10141 tgttgtttgc tggggtgcgc agcacgttac gcttgtagtt ctggatggtc acatggtcgg
    10201 tgtgcgccga gtcgtgcttt accccggcga tatacggtcg gtccgggttg ccttcctcaa
    10261 acgcgatgct gacctccgtt cccgccagca gcggcagatg caggccgtag gtgtctcccg
    10321 catacgggcg cgactggcgt acccacaggc tctcataacc cggtttccac gtatcgcggt
    10381 caaaatcgag gttgacccgg tagcgcccgt ctttgtcgat atgggcatag atatcgttct
    10441 tcaccgtgct ggtgacccgc gccgggagcg ttccggccat gaccgggcgg gggataaacg
    10501 ccgggcggta gccgtagcgt tctgagtacg ggatagcggt aaacgtcagt tcatagctgc
    10561 ggtcacgcgc cgcgctggcc gtcacgccgg taatcagtac gcccttgcgg aacaccgctg
    10621 gcgcgtcgtc gccctgaacc ctgatttcca gccccggcat cagcagtgaa ctggtgctct
    10681 ggcctttgag aatggcctgt tcattcaggt agcgttcgtg gcggatacgg gcataaaacg
    10741 cgccgctttc cgctgcctct ttatcgcctt tttgcaggaa gttgtcggcg tagtgatagg
    10801 cttcgccgta ggtggtgtta tcgcccccgg tggcgtcatg ctgctctgtc atcatctccg
    10861 ctgtggcggt gcggtagttg tagtcccgcg tggtgacgct tttttccacc acgctgtacg
    10921 cggtgttcag cccccatacg gcttccgttt caccatcaaa cagccctgaa ggatgacgaa
    10981 gcggcagcgt cagcccgcgc tcatagccgc tctggtcgtc ataaaactcg acaacctcaa
    11041 ttttcagccg ggcgtcggtg gcgaagcgga accagatgcc cacttccgac agcaggcgtg
    11101 aaataaacgt caggtcgtcc tcgccatact gcatcacctg ttcgcgggcc gggtattcgc
    11161 ttttgaggtt aaacacgaag tcctgaccgc gcatctggtg ccgctcgcgc aggatttttt
    11221 cgacgatttg cggcaccgtc agattctggt agatggcatt ctggcggctg cgggccagca
    11281 gcgccatacg cggctccagc cgtacttcgt aacgggcttc gtcctgtgac gatgacagat
    11341 gtttaaagcc ggtaatgacg ccatacaggg tgcgcagggg ggcctgcacc ggcattccct
    11401 gtaccggcgg tgccgtcagg ctgaatgcgc cgtcctgcat cagcaccaac tccggcgtga
    11461 tggctttatc ggtactggta aactgaatgt cgtagcggaa cggggtactc agttgttcgc
    11521 ggccttcgaa ggtcagcaca tccaccggac tttgcaggcc gcggattttg agtttgtggt
    11581 gtgaatggtc aaagtttagg gcaggaatac tcatcattat ttccctgtat agatagaaat
    11641 attttcgtca ttaaaaataa atcaacaatg aaactcaatg aactctcgcc attgtggtgt
    11701 cgcattattt ttcactaaga aatcgtttct ttcatcaggt gatagactgt gccagaacgg
    11761 agaccagata ctctcccacc agaactcaag ttctccttgt aagctaccga aagtatcggg
    11821 ttccaactca ggaaaaacat cccatggtac aggttctttt attccagcag atttaatagt
    11881 ttcaacaaaa aaagaagagt tcgtcactaa atcaatcaaa ttcccttt
//
LOCUS       DAZGQL010000132        11534 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.132, whole genome shotgun sequence.
ACCESSION   DAZGQL010000132 DAZGQL010000000
VERSION     DAZGQL010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11534
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.132"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..995)
                     /gene="pyrD"
                     /locus_tag="ACHKIR_004498"
     CDS             complement(<1..995)
                     /gene="pyrD"
                     /locus_tag="ACHKIR_004498"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HFO9992144.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSQELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKE"
     gene            1232..1807
                     /gene="ssuE"
                     /locus_tag="ACHKIR_004499"
     CDS             1232..1807
                     /gene="ssuE"
                     /locus_tag="ACHKIR_004499"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_process: GO:0046306 - alkanesulfonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFO9992145.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFVP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSTLKAQEILHGVFADDSQVIDYQHKPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLLSLRGNAHA"
     gene            1800..2759
                     /gene="ssuA"
                     /locus_tag="ACHKIR_004500"
     CDS             1800..2759
                     /gene="ssuA"
                     /locus_tag="ACHKIR_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HFO9992146.1"
                     /translation="MRKIIKLALAGLLSVSTLAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQTKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLTTFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     LSAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            2756..3901
                     /gene="ssuD"
                     /locus_tag="ACHKIR_004501"
     CDS             2756..3901
                     /gene="ssuD"
                     /locus_tag="ACHKIR_004501"
                     /EC_number="1.14.14.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008726 - alkanesulfonate monooxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HFO9992147.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAH
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRNVAQS"
     gene            3913..4704
                     /gene="ssuC"
                     /locus_tag="ACHKIR_004502"
     CDS             3913..4704
                     /gene="ssuC"
                     /locus_tag="ACHKIR_004502"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HFO9992148.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVMAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            4701..5468
                     /gene="ssuB"
                     /locus_tag="ACHKIR_004503"
     CDS             4701..5468
                     /gene="ssuB"
                     /locus_tag="ACHKIR_004503"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HFO9992149.1"
                     /translation="MNTARLNQGTPLLLNAVSKQYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQEDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQEHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMQRGHSEQPIRRHG"
     gene            complement(5674..8286)
                     /gene="pepN"
                     /locus_tag="ACHKIR_004504"
     CDS             complement(5674..8286)
                     /gene="pepN"
                     /locus_tag="ACHKIR_004504"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HFO9992150.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDVPLRLNGEDLKLVSVYINDEPWTAWKEEEGALVISNLPERFTLKIVNEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKTKYPFLLSNGN
                     RVAQGELENGRHWVRWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQLLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            8552..9754
                     /gene="pncB"
                     /locus_tag="ACHKIR_004505"
     CDS             8552..9754
                     /gene="pncB"
                     /locus_tag="ACHKIR_004505"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFO9992151.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQVQAMQYLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLADFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            9923..11323
                     /gene="asnS"
                     /locus_tag="ACHKIR_004506"
     CDS             9923..11323
                     /gene="asnS"
                     /locus_tag="ACHKIR_004506"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFO9992152.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
BASE COUNT         2645 a         2987 c         3045 g         2857 t
ORIGIN      
        1 atttctttaa tcagcggcgg acctttaaaa ataaaaccag aataaatttg caccagtgag
       61 gcccccgcag caatcttttc acgcgcagcg ataaccgaat caatgccgcc aacaccgatg
      121 atcggtaagc gaccgtttaa ttcctgtgac aagcggcgaa taatttctgt gctttttaac
      181 tgaagcggac gaccacttaa gccaccggtt tgatcacaat ttttcattcc ctgaaccaga
      241 gaacgatcga gcgtggtatt ggttgcaatc acgccatcaa tattatggcg aactaaacta
      301 tcggcaacct ggatcaattc ttcttcagaa agatccggcg cgatcttcac tgcgatcggc
      361 acatatttat ggtgcatcgc ttgcaaatca ttttgcttat ttttaatcgc ggttaagaga
      421 tcatccagcg cttcgccata ttgcagtgtg cgtaatcctg gggtatttgg cgatgaaata
      481 ttgatggcga tatatcccgc ataggcatag attttttcca tacaaatcag atagtcatct
      541 ttaccctgct ccaccggcgt atctttattt ttgccgatgt taatacccag aacgccgtca
      601 taatgggctt tttttacgtt ctctacgagg ttatcaacgc caagattatt aaagcccata
      661 cggttgatca aaccttcggc atctaccaga cgaaagagac gcggcttgtc attacctggc
      721 tggggacgtg gcgtgacggt accgatctcg atcgatccaa atcccatcgc gcctaacgcg
      781 tcaatgcact ccccgtcttt atcaagaccg gctgccagac caagcggatt tttaaacgtc
      841 aggcccatgc agttaacagg tttcgcaggc actttctgcc gcaccagtgc ttcaaacggc
      901 gttccggtaa tacggcgtaa ttgctgaaaa gtaaactcat gagcgcgctc tggatcgagc
      961 tggaaaaggg ctttacgaac gaaggggtag tacatgaact ctcctggatt cccggtgtgc
     1021 aaaccggggg cgtattatgt gcgagttcgg accaaaaggg aattgacctg cggcaataaa
     1081 tagcaaacgt tttcttattg cgcttttttt atgctttttt tggcttttct tcctcagata
     1141 aatcatttcg actcgcggac gccctctgtc agactgaccg aattgttatc aatgttaaca
     1201 aaacaagaac aattggttat aaggagagag tatgcgtgtc atcaccctgg cgggtagtcc
     1261 tcgctttcct tctcgctcca gctccttgct ggaatatgcg cgggaaaaac taaatggcct
     1321 ggatgtagag gtttatcact ggaatttgca aaacttcgtc ccggaagatc tgctttatgc
     1381 tcgtttcgat agtccggcac tcaagacctt caccgaacag ctgcaacagg ccgatgggct
     1441 gattgtcgcc acgcctgtgt ataaagccgc ctattccggc gcgctgaaaa ccctgctcga
     1501 cctgctgcca gaacgtgcgt tgcaaggcaa agtggtgctg ccgctggcga caggcggcac
     1561 cgtggcccat ctgctggcgg tcgattatgc ccttaagcca gttttaagta cgctgaaagc
     1621 ccaggagatc ctgcacggcg tgtttgccga tgactcgcaa gtaattgatt accaacacaa
     1681 accccagttc acgccaaatc tgcaaactcg tcttgatacc gcgctagaaa ctttctggca
     1741 ggcgttgcac cgccgcgatg ttcaggttcc tgaccttctg tctctgcgag gtaatgccca
     1801 tgcgtaagat cattaaactg gcgctggcgg gtttacttag cgtctctaca cttgctgttg
     1861 ccgcggagtc ttcgccagag gcgttacgta taggctatca aaaaggcagt attggtatgg
     1921 tgctggcaaa aagccaccag ttactggaaa aacgctatcc gcaaacaaaa atctcctggg
     1981 tggagttccc cgctggccca cagatgctgg aggcgttaaa cgttggcagt atcgatctcg
     2041 gcagtaccgg ggatattccg ccaatctttg cccaggctgc cggggctgat ttggtatacg
     2101 tgggcgtcga gccaccgaaa cccaaagccg aagtgattct ggtggcagaa aacagcccga
     2161 tcaaaaccgt agccgatctt aaaggtcaca aagttgcctt tcagaaaggt tccagttcgc
     2221 acaatctttt actgcgagcc cttcgccagg ccgggcttaa attcacggat attcagccca
     2281 cttacctgac gcctgccgat gcccgcgccg cgttccagca aggtaacgtt gacgcctggg
     2341 ctatctggga tccctactac tccgctgcat tattacaggg cggcgtgcgg gtgctgaaag
     2401 acggcaccga tctcaatcaa actggatcgt tttatctggc agctcgtccg tatgcagaaa
     2461 aaaacggcgc ttttattcag ggtgtgctga caacctttag tgaggccgat gcgttaaccc
     2521 gcagccagcg cgaacaaagc atcgctttac tggcaaaaac gatgggctta ccggcaccgg
     2581 ttattgcctc atatctggat catcgtcctc ccaccaccat caaaccgttg agcgctgaag
     2641 ttgccgcctt acagcagcaa acggcagatc tgttttatga aaaccgtctg gtgccgaaaa
     2701 aagtcgatat tcgccagcgc atctggcagc ccactcaact ggaaggaaaa caattatgag
     2761 tctgaatatg ttctggtttt taccgaccca tggtgacggg cactatctgg gaacggaaga
     2821 aggttcacgt ccggttgatc acggttatct gcaacaaatt gcgcaagcgg cggatcgtct
     2881 gggttatacc ggtgtgctga tcccaacagg acgatcgtgt gaagatgcgt ggctggttgc
     2941 cgcgtcgatg atcccggtga cgcaacggct gaagtttctt gtcgccctgc gtcccagcgt
     3001 aacctcacct accgttgccg cccgccaggc cgccacgctt gaccgtctct cgaatggtcg
     3061 tgcgttgttt aatctggtca caggcagcga tccacaagag ctggcaggcg acggcgtgtt
     3121 ccttgatcat agcgagcgct acgaagcctc ggcggaattt acccaggtct ggcggcgctt
     3181 attgctgggg gaaaccgtca atttcaacgg taaacatatt catgtgcgcg gagccaaact
     3241 gctcttcccg ccgattcaac agccgtatcc tccgctttac tttggcggat cgtcagatgt
     3301 cgcccaggag ctggcggcag aacaagttga tctctacctc acctggggcg aaccgccgga
     3361 actggttaaa gagaaaatcg aacaagtgcg ggcgaaagct gccgcgcatg gacgcaaaat
     3421 tcgtttcggt attcgtctgc atgtgattgt tcgtgaaacc aacgacgaag cgtggcaggc
     3481 cgccgagcgg ttaatctcgc atcttgatga tgagactatc gccaaagcac aagccgcatt
     3541 cgcccggacg gattccgtag gacaacagcg aatggcggcg ctacataacg gcaagcgcga
     3601 caatctggag atcagcccca atttatgggc gggcgttggc ttagtgcgcg gcggtgccgg
     3661 gacggcactg gtgggcgatg gtccgacggt cgctgcgcga atcaacgaat acgccgcgct
     3721 tggcatcgac agctttgtgc tttcgggcta cccgcatctg gaagaagcgt atcgggttgg
     3781 cgagttgctg ttcccgcatc tggatgtcgc catcccggaa attccccagc cgcagccgct
     3841 gaatccgcaa ggcgaagcgg tggcaaatga ttttatcccc cgtaacgtcg cgcaaagcta
     3901 aggaatacga taatggcaac gccagtgaaa aagtggttat tgcgcgttgc cccctggttt
     3961 ttaccggtgg gcatcgtggc ggtgtggcaa ctggcctcct cagttggctg gctgtcaacg
     4021 cgtattttgc cctcaccgga aggagtggtg atggcgttct ggacgctctc cgccagcggc
     4081 gaattgtggc agcatctggc gatcagctcc tggcgggcgc tcattggctt ttcaattggc
     4141 ggatcgctgg ggttgatttt gggactgatt agcgggctat ctcgctgggg agaacgactg
     4201 ctggatacct ccattcagat gttgcgtaac gtgccgcatc tggcgctgat cccgctggtg
     4261 attttgtggt ttggcattga tgaatccgca aaaatctttc tggtggcgct cggcacgcta
     4321 ttccccattt atatcaacac ctggcacggc atccgtaata tcgatcgcgg tctggtggaa
     4381 atggcgcgta gctatggatt atccggcata ccgctgttta tccatgtgat cctgcctggc
     4441 gccctgccct caattatggt cggcgtgcgt tttgctttgg gcctgatgtg gctgacgctg
     4501 atcgttgccg aaactatttc tgccaattcc ggcattggtt atctggcgat gaatgcgcgg
     4561 gagtttctgc aaacggacgt ggtggtagtc gctattattc tttacgccct gctcggcaaa
     4621 cttgctgacg tcagcgcgca gctgttagag cgtctctggt tgcgctggaa cccggcttat
     4681 catttgaagg aggccacggt atgaatactg ctcgtctgaa ccagggcaca ccattgttgc
     4741 tcaatgcagt aagcaaacaa tacgcggaaa atatcgtcct gaaccaactg gatttacata
     4801 ttccggcagg tcagtttgtg gcggtggtgg gccgcagcgg tggtggcaaa agtaccctgc
     4861 tgcgcctgct ggcaggtctg gaaacgccaa ccgcaggcga cgtgttagcg ggcaccacac
     4921 cgctggctga gattcaggaa gacacgcgaa tgatgtttca ggatgcgcgt ctgctaccgt
     4981 ggaaatcggt gattgataac gttgggttag gccttaaagg ccagtggcgc gatgccgcgc
     5041 gtcgggcgtt ggctgcagta ggactggaga accgcgcagg ggaatggcct gccgcacttt
     5101 ctggcgggca gaaacagcgt gtggcgctgg caagagcgtt aattcatcga ccgggtttat
     5161 tattgcttga tgaaccgctc ggggcgctgg acgccctgac tcggctcgag atgcaggatt
     5221 tgattgtgtc actttggcag gagcacggct ttaccgtact gttggtgacg catgatgtga
     5281 gcgaagcggt agcaatggct gaccgggtat tgttaattga agagggaaaa attggtctgg
     5341 atttgacggt ggatatcccc cgtccgcgcc ggttagggtc ggtgcgatta gcggagctgg
     5401 aagcggaagt tttgcaacgg gtgatgcagc gtgggcactc tgaacaaccg attcggcgtc
     5461 atggttaata tgccggatgt ggcacacaaa accagcagtt tcaacggatt gtaggcctga
     5521 taagacgcgt caagcgtcgc atcaggcatt tgggcacggc tgccggatgc ggcgtgaacg
     5581 ccttatccag cctacaaaac cagcattttc aacggacttg taggcctgat aagacgcgtc
     5641 aagcgtcgca tccggcattt tggctcaaaa gtatcaagcc agtgctttag ttatcttctc
     5701 gtacagatcg ccagagagat tttccagccc tttcaactgc tccagcgccg cgcgcatttt
     5761 ctcctgacgt ttggcatcgt aacgtttcag gcgaatcagc ggctcaatca ggcgtgaagc
     5821 cacctgcggg ttacggctgt tgaggtcggt aagcatttcc accaggaact ggtaaccgct
     5881 gccatcttcg gcatggaacg ctgccgggtt gctgcccgca aacgcaccaa tcaacgaacg
     5941 aatacggttc gggttgctca tggtaaatga gcgatgctgc aacaggccac gcaccgtctc
     6001 cagcacattc gccgccgggc tggtggcttg caggataaac catttatcca tcaccagacc
     6061 atcctgatgc cacttgtcgt cgtactcctg catcagcgcg tcacggcaag gcagctgtgc
     6121 agcaaccgcc gcagaaagcg ctgccagcgc atcggtcata ttgtttgctt catggtattg
     6181 cttgctcacc agcacgtcag ccagatgcgt ttcaccaaaa gcaaggaagc gcaggcaggc
     6241 attacgcaga gtgcgttttg caatatcttc atgctcaaca cggtactcgc tctggtaatt
     6301 cgcgttgtaa atagccagta gctcatccgc cagttcagtc gccagagtac gagtgagtgc
     6361 ttcgcgtact tcggcaatag caatcgggtc gatgatatcg aacagttcag ccatttcatt
     6421 gacagaaggc agcgtcagga tttctgccgc cagcgctgga tcaatcttct catcgagcag
     6481 caccgcgcgg aaagcgtcag ccacatgcac cggcagagac agcagctgcc cttgctgatg
     6541 acgggcgacg tttagcttga tgtaggttgc cagcaggctt tgcgccgcat cccaacggga
     6601 gaaatcatta cgcgcatgac gcatcaggaa ggtcagttgc tgatcgctcc acttatattc
     6661 cagtttcact ggcgcagaga attcgcacag cagcgcaggc accggctgga agtagacatt
     6721 atcaaaaaca aaggtctgtt cagcctgggt gacgttcagc accgaattca ccggatgacc
     6781 gcctttctgc aacgggatca ctttgccttc gttatcatac agttcgatgg caaacggaat
     6841 atgcagcggc tgtttttctg cctgatccgg cgtagctggc gtgcgctggc tgatggtcag
     6901 ggtgtactgc tcagtttccg gattgtagtc gtctttgaca gtcacaaccg gcgtgccgga
     6961 ttggctgtac caacggcgga aatgggaaag atcgacattc gacgcatctt ccatcgcctg
     7021 cacaaagtcg tcacaggtcg ctgcgctacc atcatgacgc tcgaaataga gctgcatccc
     7081 tttctggaag ttttcttcgc caagcagagt gtggatcata cgaatcactt ccgcgccctt
     7141 ctcgtaaacg gtcagggtgt agaagttgtt catttcaatg accatatccg ggcgaattgg
     7201 gtgcgccatc gggctggcgt cttctgcaaa ctgcaatccg cgcatggtgc gcacattatt
     7261 gatacggttt accgcgcggg aaccaaggtc agagctgaat tcctgatcgc ggaagacggt
     7321 taaaccttct ttcagactga gctggaacca gtcgcgacag gtgacacggt tgccggtcca
     7381 gttatggaaa tattcatggc cgataacgcg ttcaatatcg aggtaatctt tgtcggtggc
     7441 ggtgtcggtg cgggccagca catatttgga gttaaagata ttcagcccct tattctccat
     7501 tgcgcccata ttgaagaaat ccaccgcgac gatcatatag atgtcgaggt catactccag
     7561 gccgaagcgt tcttcatccc atttcatgga gtttttcagc gaggtcattg cccacggcgc
     7621 acgatcaagg ttgccacgat cgacatacag ctccagcgct acttcgcgac cagaacgcgt
     7681 ggtgaaagtg tcgcgcagca catcaaagtc gcctgccacc agcgcaaaca ggtagcacgg
     7741 tttcgggaac gggtcctgcc accgtaccca atgacgtcca ttttccagtt caccttgcgc
     7801 aacgcggtta ccgttggaaa gcaggaaggg atatttggtt ttatcggcaa taattttggt
     7861 ggtaaaacgc gccagcacat ccgggcggtc gagataatac gtaatatggc ggaaaccttc
     7921 ggcttcacac tgggtgcaaa gcgcatcgcc tgactgataa agcccttcca gcgcagtatt
     7981 cgccgccggg ctaatttcat taacgatctt aagcgtaaaa cgctccggca aattactgat
     8041 gaccagtgcg ccctcttctt ctttccaggc ggtccacggc tcatcattaa tataaacaga
     8101 aaccagtttg aggtcttcgc cgttgagacg aagcggaaca tctgatgcac catgacggac
     8161 agcctggctg accgcggtaa cgaccgtctt ttgcgcgtcc aggtcaaagg tcaagtcaat
     8221 atcagtaatc tggtaatccg gcgcacgata atcgtgacgg tatttggctt gtggctgttg
     8281 agtcataaat aacctttagc atcttttata gagtctggtg ttcagtctat tcctgttgcg
     8341 taaatcgcgc tatgcagaat cttcatcttt tcaggtacaa acgcctttat tgctacattt
     8401 ttataacata caccgcgtaa tgccatcgac cagaaaggtg gcatatggtg tgatcggggt
     8461 tcaataaatt gcgaaacaag gtatactcca gcagttcctg aagatgttta ttgtactaaa
     8521 cgctcctgta cgaggacgct actgcgcacc tatgacacaa ttcgcttctc ctgttctgca
     8581 ctcgttgctg gatacagatg cttataagtt gcatatgcag caagccgtgt ttcatcacta
     8641 ttacgatgtg catgtcgcgg cggagtttcg ttgccgaggt gacgatctgc tgggtattta
     8701 tgccgatgct attcgtgaac aggttcaggc gatgcagtac ctgcgcctgc aggatgatga
     8761 atatcagtgg ctctctgccc tgcctttctt taaggccgac tatcttaact ggctacgcga
     8821 gttccgcttt aacccggaac aagtcaccgt gtccaacgat aatggcaagc tggatattcg
     8881 tttaagcggc ccgtggcgtg aagtcatcct ctgggaagtt cctttgctgg cggttatcag
     8941 tgaaatggta catcgctatc gctcaccgca ggccgacgtt gcgcaagccc tcgacacgct
     9001 ggaaagcaaa ttagccgact tctcggcatt aaccgccggt cttgatatgt cgcgcttcca
     9061 tctgatggat tttggcaccc gccgacgttt ttctcgtgaa gtacaagaaa ccatcgtgaa
     9121 acgtctgcaa caggaatcct ggttcgtggg caccagcaac tacgatctgg cgcgtcggct
     9181 ttccctcacg ccgatgggaa cacaggcaca cgaatggttc caggcgcatc agcaaatcag
     9241 cccggatcta gccaacagcc agcgagctgc acttgctgcc tggctggaag agtatcccga
     9301 ccaacttggc attgcgttaa ccgactgcat cactatggat gctttcctgc gagatttcgg
     9361 tgtcgagttc gccagtcgct atcagggcct gcgccatgac tctggtgacc cggttgaatg
     9421 gggtgaaaaa gccattgcac attatgaaaa gctgggaatt gatccacaga gtaaaacgct
     9481 ggttttctct gacaatctgg atttacgcaa agcggttgag ctataccgcc acttctcttc
     9541 ccgcgtgcaa ttaagttttg gtattgggac tcgcctgacc tgcgatatcc cccaggtaaa
     9601 acccctgaat attgtcatta agttggtaga gtgtaacggt aaaccagtgg cgaaactttc
     9661 tgacagccca ggcaaaacta tctgccacga taaagcgttt gttcgggcgc tacgcaaagc
     9721 gttcgacctt ccgcatatta aaaaagccag ttaatatcat cagggagcta atcggctccc
     9781 tttttttacc tttaattccg aaatctttcg ctgcatttgc gaattctgct tgtctgattg
     9841 cagatgccag gtaacatagg tatcccccca tttacgggtg gacaagtgtt tattttttcc
     9901 gactattaac agagagaata ttatgagcgt tgtgcctgta gccgacgtac tccagggccg
     9961 cgtagccgtt gacagcgaag tcaccgtgcg cggatgggta cgtacccgcc gagattcaaa
    10021 agctggcatc tccttcctcg ccgtttatga cggttcctgc tttgatcctg tacaggctgt
    10081 catcaataat tctctgccca attacaatga agacgtcttg cgtctgacca ccggctgctc
    10141 ggtcattgtg acgggtaaag tcgtggcgtc gccgggccag gggcaacaat ttgaaattca
    10201 ggccagcaag gttgaagttg ctggttgggt tgaagatcca gacacttacc cgatggcggc
    10261 aaaacgccac agcattgagt atctgcgtga agtcgctcac ctgcgtccgc gcacaaacct
    10321 gattggtgcc gtcgcgcgcg ttcgccatac gctggcgcag gcgctgcatc gcttctttaa
    10381 cgagcaggga tttttctggg tttcaacgcc actgattacc gcatctgata ccgaaggtgc
    10441 aggcgaaatg ttccgtgttt ctacgctgga tctggaaaat ctgccgcgta acgatcaggg
    10501 caaagtggat ttcgacaaag acttctttgg taaagagtct ttcctgaccg tatctggcca
    10561 gttgaacggc gaaacctacg cttgcgcatt gtccaaaatt tataccttcg gcccgacttt
    10621 ccgtgctgaa aactccaaca ccagccgtca cctggcggaa ttctggatgc tggagccgga
    10681 agtggcgttt gctaacctga acgatatcgc gggtctggct gaagccatgc tgaaatatgt
    10741 cttcaaagcg gttctcgaag aacgcgctga cgacatgaaa ttcttcgctg aacgcgtaga
    10801 taaagatgcc gtttcacgtc tggaacgctt cattgaggcc gatttcgcgc aggtggatta
    10861 caccgacgca gtgaccattc tcgaaaactg cggcaggaag tttgaaaacc cggtttactg
    10921 gggcgtcgat ctctcttctg agcatgagcg ttatctggcg gaagaacact ttaaagcacc
    10981 ggtagtggtt aaaaactatc cgaaagatat taaagcgttc tatatgcgcc ttaacgaaga
    11041 cggtaaaacc gttgcggcta tggacgttct ggctccgggc atcggtgaga tcattggtgg
    11101 ctcccagcgt gaagaacgtc tggacgtgct ggacgagcgt atgctggaaa tgggcctgaa
    11161 taaagaagat tactggtggt atcgcgatct gcgtcgctac ggtactgttc cgcattcagg
    11221 tttcggtctt ggttttgaac gcctgattgc ttacgtaact ggtgtgcaaa acgtgcgtga
    11281 tgtgattccg ttcccacgta ctccgcgtaa cgccagcttc taatttagcg tcttcaagag
    11341 ccagcacccg ctggctcttt ttttatcctt tctgtcaagt tatctgtttg ttaagtcaag
    11401 caatctattt gcaaccccgc cataacgatc atcctgttac ggaatattac attgcaacat
    11461 ttacgcacaa aaaataaccc gcattcttat tgcggattag ttttttctta gctgatagca
    11521 caattttcat acta
//
LOCUS       DAZGQL010000133        11486 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.133, whole genome shotgun sequence.
ACCESSION   DAZGQL010000133 DAZGQL010000000
VERSION     DAZGQL010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11486
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.133"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(150..956)
                     /gene="trpA"
                     /locus_tag="ACHKIR_004507"
     CDS             complement(150..956)
                     /gene="trpA"
                     /locus_tag="ACHKIR_004507"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HFO9992153.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNLAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVTKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKAFVQPMKAATRS"
     gene            complement(956..2149)
                     /gene="trpB"
                     /locus_tag="ACHKIR_004508"
     CDS             complement(956..2149)
                     /gene="trpB"
                     /locus_tag="ACHKIR_004508"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HFO9992154.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPEKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(2161..3522)
                     /gene="trpCF"
                     /locus_tag="ACHKIR_004509"
     CDS             complement(2161..3522)
                     /gene="trpCF"
                     /locus_tag="ACHKIR_004509"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HFO9992155.1"
                     /translation="MMQTVLAKIVADKAIWVEARKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPAHIATVYRNYASAISVLTDEKYFQGSFDFLS
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDEQYRHLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLNAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPARELQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            complement(3523..5118)
                     /gene="trpD"
                     /locus_tag="ACHKIR_004510"
     CDS             complement(3523..5118)
                     /gene="trpD"
                     /locus_tag="ACHKIR_004510"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HFO9992156.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHVPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDSQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPTNTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(5118..6680)
                     /gene="trpE"
                     /locus_tag="ACHKIR_004511"
     CDS             complement(5118..6680)
                     /gene="trpE"
                     /locus_tag="ACHKIR_004511"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HFO9992157.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCEDRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVENEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQSLLNVPNEEREAMFFGGLFSYDLVAGFED
                     LPQLAAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRSLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     misc_feature    complement(6702..6795)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            complement(6772..6816)
                     /gene="trpL"
                     /locus_tag="ACHKIR_004512"
     CDS             complement(6772..6816)
                     /gene="trpL"
                     /locus_tag="ACHKIR_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HFO9992158.1"
                     /translation="MKAIFVLKGWWRTS"
     gene            6954..7835
                     /gene="rnm"
                     /locus_tag="ACHKIR_004513"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
     CDS             6954..7835
                     /gene="rnm"
                     /locus_tag="ACHKIR_004513"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
                     /EC_number="3.1.13.-"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase AM"
                     /protein_id="HFO9992159.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAISPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQLIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HYGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            7832..8452
                     /gene="yciO"
                     /locus_tag="ACHKIR_004514"
     CDS             7832..8452
                     /gene="yciO"
                     /locus_tag="ACHKIR_004514"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFO9992160.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            8480..10369
                     /gene="yciQ"
                     /locus_tag="ACHKIR_004515"
     CDS             8480..10369
                     /gene="yciQ"
                     /locus_tag="ACHKIR_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HFO9992161.1"
                     /translation="MAGIYRCILLLIVGLFFSSLSYPKNTEIPFYEEGISLFDVEATL
                     QPDGVLDIKENIHFQARNQQIKHGFYRDLPRLWMQPDGDAALLNYHIVGVIRDGIPEP
                     WHLDWHIGLMSIVVGDKQRFLPQGDYHYQIHYQVKNAILREGDSDLLIWNVTGNHWPF
                     EIYKTLFSLKLPDIAGNPFSEIDLFTGEEGDTYRNGRILEDGRIESRDPFYREDFTVL
                     YRWPHALLGNAPAPQTTNIFSHLLLPSTSSLLICFPSLFLACGWLYLWKRRPQFTPVD
                     VIETDVIPPDYTPGMLRLDAKLVYDDKGFCADIVNLIVKEKIHLEDHYDKNQQILIRV
                     NEGATRNNAVLLPAEQLLLEALFRKGDKVVLTGRRNRVLRKAFLRMQKFYLPRKKSSF
                     YRPDAFLQWGGMAILAVILYGNLSPVGWEGMSLVGDMFIMICWLLPFLFCSLELLFAR
                     DDDKPCVNRVIITLFLPLICSGVAFYSLYINVGDVFFYWYMPAGYFSAVFLTGYLTGM
                     GYIFLPKFTQTGQQRYAHGEAIVNYLARKEAATHSGRRRKGETRKLDYALLGWAISAN
                     LGREWALRIAPSLSSAICAPEIARNGVLFSLQTHLSCGAYTSLLGRSYSGGGSGAGGG
                     GGGGW"
     gene            10582..11457
                     /gene="rluB"
                     /locus_tag="ACHKIR_004516"
     CDS             10582..11457
                     /gene="rluB"
                     /locus_tag="ACHKIR_004516"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HFO9992162.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEI
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHC
                     QVSGGRRSGGRNNNG"
BASE COUNT         2711 a         2916 c         3060 g         2799 t
ORIGIN      
        1 atattactgt tgggcggaaa aaagacgtaa gttgacgttc gacaggggca agcgaaacgg
       61 taaaaagata aataataaat gaatttagga tttttccagc ttcgttaaag aaagttaaaa
      121 tgccgccagc ggaactggcg gctgtgggat taactgcgcg tcgccgcttt catcggttgt
      181 acaaaagctt tcagtgccgc cagcattttc tctggctcat taatatgttg ctcgatgatt
      241 ttaacgatgg ccgaaccaga aatcgcgccc gcagctcctg catcaatcgc tgcttttacc
      301 tgatccgggg cggaaatacc aaagccctgc aatggaggcg cagcattgta ttctttcagc
      361 ttcgtgacca gatgattgag gggtaacgcg gcgcggtttt ctgcgccggt cacgcccgca
      421 cgtgacaata aataggtgta tccacgaccg taggaggcta tctggcgcag caaatcatcg
      481 tcggcattcg gcgggcagat aaagataggt gcaagattat gacgcaacgc ggcctggcgg
      541 aagggagcgg actcttccac tggcacatcg gcaaccagca ccgaatcgac gccgactttc
      601 tcgcactggg cataaaactc atcaatgcct ttgttaaaca ccaggttggc atacatcagc
      661 aggccaatgg gaatggtcgg gtgtttctgg cgaatcagtg ccagcatttc aaaacattgt
      721 gccggagtca cacctgccgc aaaggcgcgc agagtggcgt tttgaatcgt cgggccatcc
      781 gccagtgggt cggagaaggg gatacctaac tccagcgcgt cagcaccggc ttcaattagc
      841 gtgtcgataa ttttcagcga ctgctcaatg cccggatcac cgagcgtgac gaaaggaacg
      901 aatgcgcctt ctttgcgctc cttcaactgg gcaaacagag attcgtagcg ttccatcaga
      961 tttcccctcg tgctttcaaa atatcgtgaa cggtgaagat gtctttatcg ccgcgaccgg
     1021 aaaggttaac caccagtagc tgctcttttt ccgggttttc gcgcatcatt ttcagcgcat
     1081 gggccagggc gtgtgaggac tccagcgccg ggataatccc ttcgtgcagg cagagcgttt
     1141 tgaaggcttc cagtgcttca tcatcggtaa ttgacacgta atcagcgcgt ccagtgctgt
     1201 taagatacgc gtgttgtggg ccgacagacg ggaaatccag tccggcggag atggagtaag
     1261 attcttcaat ctgcccgtct tcggtttgca tcatcggcgc tttcataccg aaatagatgc
     1321 ccacgcgacc gtgttttaac ggtgcgccgt gctcgccagt ttcgataccg tgaccacctg
     1381 gctccacacc aatcaggccg acgttggttt cattgatgaa atcagcaaac atgccgatgg
     1441 cattcgaacc gccgccaaca caggcgataa cagcatccgg caggcgacct tctctttcca
     1501 gaatctgcgc tttcgtttct tcgccaatca tccgctgaaa ctcacgcaca atggtcgggt
     1561 aaggatgcgg gccagccgcg gtgcccagca tatagtgcgc ggtttcataa ctgccggacc
     1621 agtcgcgtag cgcctcatta caggcatctt tcagcgtcgc ggaaccgcta tgcaccggga
     1681 tcacttccgc acccattaaa cgcatacgaa aaacgttagg cgactggcgt tcaacgtctt
     1741 tggcacccat ataaatgcga catttcaggc cgagcagggc gctggcaagg gccgacgcca
     1801 cgccatgctg accggctccg gtttcggcga tgatttccgt tttacccatc cgcttcgcca
     1861 gcaacgcctg gccgagcact tggttggttt tatgcgcgcc gccgtgcagc aaatcttcgc
     1921 gcttgagata cagcgtggtg ttagtgccag ccgtaatgtt ctggcatttg gtgagtgcgg
     1981 ttggacgccc ggcatagttt ttcagcaggt cgttgaactg agcctgaaat tcaggatctt
     2041 tttgcgcgct aacaaaagct tcttccagct ggcgcagagc aggcatcagg atttgtggca
     2101 cgtacatgcc gccaaactca ccaaaatacg ggttaagtaa tgttgtcatt gttcctttcc
     2161 ttaatatgcg cgcagcgtct ggaaaaccga ggccaaaaga cgtgcgtctt tgatgcccgg
     2221 ttgcgactct acagcagaat taaaatcaag tccggcgcag ccggtttgtg ccgcttccac
     2281 gcagttatct gcgcctaagc cccccgccag cagaacgttg ccaagcgatt gaccgtttaa
     2341 cagtgaccag tcgaaacgtt gaccgcttcc accctggccg ttgtctaaaa catatttatc
     2401 aacgtgctga agctcgcggg cgggcagggt ttcaccgacg cttaatgctt tccagatggc
     2461 aacgtgtgct ggcagagctt cacgcagcgt atcgatatac agctgatctt cattaccatg
     2521 cagttgcact gccgccagcg ataacacctt agctttgtcc accacatcgg caatatcgtg
     2581 attgcggaac acgccaacat actgcaacgg tgccgcagcc atcacttcct gcgcctgttc
     2641 aacgttgacg caacgcggtg atgtcgcaac aaaaatcaac ccaccgtaaa tcgcgcccgc
     2701 gtcataagct gctttagcat cttgcccacg cgtcaggcca catactttat tctcacccag
     2761 caatacccgg cgcacggcgg cgttcaaatc gtcatgggcc atcaacgccg aaccaatcag
     2821 aaaaccgtta gcgaagtggc ttaactcgcg cacctgagcg taagtattga tgccggattc
     2881 gctgattacc gtcacgttgt gccccagttt cggcgcaagc tcgcgggtac ggttgagatc
     2941 aatcgacaaa tcacgcagat cgcggttgtt gatgccaacg acctttgccc ccaatgcaat
     3001 ggcgcgctcc agttcctctt cattactgac ttcggtcagc acacccatct ccagactgtg
     3061 ggcgacggcg gcaaggtggc gatattgttc gtcatccagt actgaaagca ttaataagca
     3121 ggcatcggcc tggtaatagc gcgccagata gatctggtaa gggtcgataa taaagtcttt
     3181 acataaaatc ggctgcgggg cgatttggct gacgatggag aggaaatcaa agctcccctg
     3241 aaaatatttc tcatcagtca gcaccgaaat tgccgaagcg taatttctgt agacggttgc
     3301 aatgtgtgcc ggatcaaaat catcgcggat cacgcctttt gacggtgacg cttttttaca
     3361 ctccagaata aacgccgtgc gtgcaccctg tagcgcatca taaaaatgtc gcgtgctcgg
     3421 ctgaacctca ttctgaaaac tggccagcgg ttgctgctgt ttgcgggctt ctacccaaat
     3481 cgccttgtct gcgacgattt tcgctaaaac ggtttgcatc atttaccctc gtgccgccag
     3541 tgcggtgact ctgtcgtaag cggaaccact gcgcagtacc tcaagaacgg tttgcgcatt
     3601 ggcttgcaga tcttcatggc catgcaggcg cattaacatg gcgacgttcg ccgcgacggc
     3661 tgcttcatgg gcggcgtcgc ctttaccttg taacaagcgt gttaaaatgt cacggttttc
     3721 ttccggtgtt ccgcctgcca gttgctcctg gtggtagggt gtcaggccaa aatcttctgc
     3781 agttagctga tagctcttaa tttcaccgtc atgcagttcg gcaacgattg tcggcgcgtg
     3841 taatgaaact tcatccatcc cgccgctgtg cactaccgca gcgcgttgat accccagcac
     3901 gcgcaaggtt tcggcaatcg gcagcaccag ttccggacta taaacaccaa ttagcgccag
     3961 cggcggatgc gccgggttaa tcaatggccc caacacgttg aacagggtgc gggttttcag
     4021 ttgctggcga accggcatcg cgtggcggaa tccggtgtga tatttcggcg caaagaggaa
     4081 acagacgcct aactcatcca gcgcctggcg cgatttatcg gcgttcatat caagattaat
     4141 accgaacgcc gccagcagat cggacgagcc ggatttactg gagacgctgc ggttaccgtg
     4201 tttcgccact ttcagcccac aggccgcggc gacaaacgca ctggcggtag aaatattgat
     4261 gctgttgctg ccgtcaccgc cagtaccgac gatatcggca aacagataat ccgggcgcgg
     4321 gaacggcgcg gcgttttcca gtagcgcggt tgctgctccg gcgatctcgt tcgggtgctc
     4381 gccgcgaatt ttcatgctca ccagcgccgc cgccagttgt tccggcttca gctcgccacg
     4441 caccaccgct gaaaacagct ggtgactttc ttgttggcta agcgtctgtg cctgatacag
     4501 tttttccaga atcggttgca gcgtgttggt tggctcaagt ttctgctgcg cccaggccag
     4561 cgtttgttcc agcaggcgag cgccctgggt agtaagaatg gattccggat ggaactggaa
     4621 accgcaaacg cgatctgcat cgtgacgcac cgccatcacc atgccattaa aatgggcgtt
     4681 gatggttaaa cctgccggga tattactgcc aaccagcgag tgataacgcg ccaccggcaa
     4741 tgggtttgtt aatccggcaa acatcgcctg actgtcatgt tcaatgctcg acgctttacc
     4801 gtgaagaatt tcgcccgcct gaccgacata gcccccgtaa gcttcgacaa ttgcctgatg
     4861 tccgaggcaa atgccaataa ttggcagctt gccacgcaag cgggtgagga gttccggcat
     4921 acaaccggct tcgctcggca caccggggcc aggagaaagc atcagcaccg gattgctcat
     4981 cgtcgccagg cgttcaatta aggtctgcgc cggaacatgg ttgcggtaaa tcaccacgtt
     5041 atgaccattg ctgcgcaact gatctgccag gttgtacgta aaagagtcga tattatcgag
     5101 cagcagaatg tcagccatca gaacgtctcc tgtgcatgat gcgcggtggc aatagcgcgc
     5161 agtacagcgc gggctttatt acgggtttcg tcggcttccg actgcggaac agaatcaagg
     5221 actacgccag caccggcttg cacggtggcg ataccgtttt ccaccagcgc cgagcggatc
     5281 acaatgcagg tgtcgagatc gccatgcgcg gtaaaataac ctaccgcgcc gccgtagctg
     5341 ccgcgtcgac gaccttctgc ttcggcaatt aactgcatag cgcgcacttt cggcgcaccg
     5401 cttaatgtcc ccatattcat acaggcgcgg taagcgtgca gggcgtcgag atcgtggcgc
     5461 agctcaccga ccacgcggga gacgaggtgc atcacataag aataacggtc aactttggtg
     5521 agatcggcga cgtagcgact accgggagtg caaatacgcg ccaggtcatt acgggcgaga
     5581 tccactagca tcagatgttc agaaagctct ttatgatcgg tacgcatttc cagttcgatg
     5641 cggctgtcga ggtctctgtc cagtgaacca tcggcacgac gaccgcgtgg acgtgttccg
     5701 gcaatcgggt agatctcaat ctggcggctg gtggcgtcat acttgagcga actttccggc
     5761 gacgcgccaa acagggtgaa atcattatcc tgcataaaaa acatgtacgg gctgggatta
     5821 ctctttttca gcacgtaata ggccgccagc ggtgacgggc agggcagaga gaaacggcga
     5881 gatggcacca cctggaaaat ttctccggcg cgaatcgctt tttgcagcga acgcactacg
     5941 ccaccgaact cttcatcact ctggttacat tcacaacgca tatgcggcac ggaaaccacc
     6001 ggcagcggcg gcgcggcttc ggtcagttgc tgacgtagtt cgttcaggcg agcagtgaga
     6061 cgttgttttt cttcttcatt cggagcaaac aggctggcct gaatacgggt gctttttttc
     6121 tgatggtcaa tcaccatcag cgtttcagcg agataaaaac agaaatcagg acagttattt
     6181 tccgctgcca gttgtggtaa atcttcaaat cccgccacaa ggtcataaga gaacagaccg
     6241 ccgaagaaca ttgcttctcg ttcttcattc ggtacattca acagactctg caataagcgg
     6301 aaagcgtcaa aaaccgaaag ggagcataag cgagcgtctt catccagcag tggactgaca
     6361 ggggggaagc gcagcacacg gcagtttggt gattgttcat tttccacacc cgcaggcagg
     6421 gcgttatcca gtagtgccag cagggcttcg ccattgccgg aaagtgcctg gattgtgaca
     6481 gtgtcaccta aagctgtaat gcgcagcgca ctgtctacca gcagcaggct ttttaaatca
     6541 tctttgctgt cgatatctgc ggattccagt agcagcgttg ctggacgatc ctcacacaac
     6601 tggtgaaaaa gcgcggtggg attgtcgcga taagcgcctt cgcaggttag cagttcgaga
     6661 gtcggttttt gtgtttgcat tgttattctc taattttgtt caaaaaaaag cccgctcatt
     6721 aggcgggctg ggtatctgat tgctttacgc atggtgaata cactgcccgt ttcaggaagt
     6781 gcgccaccaa cctttcagta cgaaaattgc tttcattgtc gatacccttt ttacgtgaac
     6841 ttgcgtacta gttaactagt tcgatgatta attgtcaaca gctcatttca gaatatttgc
     6901 cagaaccgtt atgatgtcgg cgcaaaaaac attatccaga acgggagtgc gccttgagcg
     6961 acacgaatta cgcagtgatt tacgacctgc acagccatac cacagcttcc gatggctgcc
     7021 tgacgccaga agcattggtg caccgtgcag tcgagatgcg cgtcggcacc ctggcgatca
     7081 ccgaccatga caccacagct gcaatctcac cagcaagaga ggaaatttca cgttcgggac
     7141 tggcgctgaa tcttattccc ggcgtggaaa tttccacggt ctgggaaaat catgaaattc
     7201 atattgtcgg gctgaatatt gatattactc atccgctaat gtgtgagttc ctcgcgcagc
     7261 agacagaacg gcgcaatcag cgggcgcagc tgattgccga aagacttgaa aaagcgcaaa
     7321 tacctggggc actggaaggc gcgcaacgac tggcgcaggg cggtgcggtg acgcgcggtc
     7381 attttgctcg tttccttgtg gagtgcggca aagccagttc aatggcggat gtctttaaaa
     7441 agtatctggc gcgcgggaaa accggatacg ttccgccaca gtggtgtaca atagaacaag
     7501 ctattgatgt cattcatcat tctggcggta aggcggtgct agctcatcct gggcggtaca
     7561 atcttagtgc taaatggctg aaaagattgg ttgcgcattt tgccgaacac tacggcgacg
     7621 cgatggaagt cgcgcagtgc cagcaatcgc ccaatgaacg cacccagctg gcgacacttg
     7681 cgcgtcagca tcatttatgg gcatcacaag gatctgattt tcatcagcca tgcccgtgga
     7741 tcgaactggg tcgtaaactc tggctgcccg caggcgttga aggcgtctgg cagctatggg
     7801 aacagccgca gaacaccaca gagagggaat tatgagccag tttttttata ttcatcctga
     7861 taacccgcag caacgcctga tcaaccaggc ggtggagatc gtgcgtaaag gcggggtgat
     7921 tgtttatcca actgattccg gctatgcgct cggctgtaaa attgaagata aaaacgcgat
     7981 ggagcgtatt tgtcgaattc gccagctgcc ggacggtcac aactttaccc tgatgtgtcg
     8041 cgatctttct gagctctcga cctattcatt cgtggataac gttgcgtttc gtttgatgaa
     8101 aaacaacacg ccgggcaact acaccttcat cctgaaaggg acgaaggagg tgccacgccg
     8161 tctgttgcag gaaaaacgca aaaccatcgg tatgcgtgtg ccttctaacc cgattgcgca
     8221 ggcgctgctt gaagcattgg gcgaaccgat gctttccaca tcactgatgc tgccaggcag
     8281 cgaatttacc gaatccgatc cggaagaaat taaagaccgt ctggaaaaac aggtggactt
     8341 gattattcac ggcggctatc tcgggcagaa accaacaacg gttattgatc ttactgatga
     8401 tacgccggtc gtggtgcgtg aaggcgtagg tgatgtgaag cctttcttat aacaaattgc
     8461 gttccacgga tggaagacta tggcagggat atatcgctgc attttgctgt tgatagttgg
     8521 gctttttttc tcttcgttga gttatccgaa aaacacggag atcccttttt atgaagaagg
     8581 gatctcgctc tttgatgttg aagccactct gcaaccggat ggggtgctcg acatcaaaga
     8641 aaatattcat tttcaggcgc gaaatcagca gattaagcac ggattttatc gtgatttacc
     8701 acgactctgg atgcagcctg atggggacgc tgcactgctg aactatcata ttgttggcgt
     8761 catccgtgat ggtattcctg aaccctggca tcttgactgg catatcgggt taatgagtat
     8821 tgtcgtgggc gataaacaac gtttcttgcc tcaaggcgac tatcattatc aaattcatta
     8881 tcaggttaaa aatgctatcc tgcgtgaggg ggattctgat ctgctaatct ggaacgtgac
     8941 cggtaaccac tggccgtttg aaatttataa gaccctattt tcactcaagt tgcctgatat
     9001 tgcgggtaat ccatttagcg aaatcgatct ctttactgga gaagagggcg acacatatcg
     9061 aaatggccgc atccttgagg acggaagaat tgaatctcgc gatccgtttt atcgtgaaga
     9121 tttcacggtc ctctaccgct ggcctcacgc attacttggt aatgccccgg ctccacaaac
     9181 gacgaatatt ttcagtcatc ttcttttacc ctcaacgtca tcgttgttaa tttgctttcc
     9241 gagtctgttc ctggcgtgtg gatggttata tctctggaag cgcaggccgc aatttacgcc
     9301 ggtagatgtg attgaaaccg atgtcattcc gccagattac acgcccggca tgttacgtct
     9361 tgatgcgaaa ctggtttacg acgataaagg tttttgcgcc gatatcgtaa atctgattgt
     9421 gaaagaaaaa attcatctgg aagatcatta tgacaagaac cagcaaatcc tgattcgtgt
     9481 taatgaaggc gcgaccagaa ataatgcggt attactgccc gcagagcagt tattactgga
     9541 agcgttattt cgtaaaggcg ataaggtcgt tcttacgggg agacgcaaca gagtcttacg
     9601 caaggcattt ttacggatgc agaaatttta tctgccgcgt aaaaagtctt cgttttaccg
     9661 acctgatgcg tttttgcaat ggggcggaat ggcaatattg gcggtcattc tctacggtaa
     9721 cctgagtccc gtaggctggg aaggaatgag tctggtgggc gatatgttta ttatgatctg
     9781 ctggcttctt ccttttttat tttgttccct tgagcttttg tttgcccgcg atgatgacaa
     9841 gccttgcgtt aatcgtgtca tcatcacttt gtttttacca ctgatttgtt caggtgtggc
     9901 tttttactct ctctacatca atgtcggaga tgtattcttt tactggtata tgccagcggg
     9961 ttattttagc gctgttttcc tgaccggtta tctcactggc atggggtata tttttctgcc
    10021 aaagtttacc caaactgggc agcaacgtta tgcccacggt gaagctatcg ttaactatct
    10081 tgcgcgtaaa gaggcagcaa cacacagtgg gcggcgtagg aaaggggaaa cacggaaact
    10141 ggattacgct ttgttaggtt gggctatatc ggcaaatttg gggagggaat gggcgttacg
    10201 catcgcccct tcgctttctt cggcgatttg cgctccagag attgcccgta acggcgtttt
    10261 attctcatta cagacgcacc tgagttgcgg ggcctatacc agtttgttgg ggcggagcta
    10321 ttctggtggt ggttctggcg ctggtggcgg aggcggtggt ggctggtaat caagctgatg
    10381 ttaatcgaaa caagctttaa ccataaagat agtgtagtat gttgcgcctc aaagccaggc
    10441 aagttaaaac gtcgagtcgt ttacttaagg cctgaagagt tcaaacagac tttactatca
    10501 gtgagtcaga tgtaaattct gactcactgt tgcacaaaag catatcagac gcctgggaag
    10561 gcgacaccaa aaggaagctc tatgagcgaa aagctacaga aagtgctggc gcgtgccggc
    10621 cacggttctc gccgtgaaat cgaatctatt attgaagccg gtcgtgtgag tgttgatggc
    10681 aaaattgcta aactcggcga tcgcgttgaa attacccctg gattgaaaat ccgtatcgat
    10741 ggtcacctga tttcggtacg tgagtccgct gaacaaattt gtcgcgttct ggcctattac
    10801 aaaccggaag gtgagttgtg tacccgtaac gatccggaag ggcgtccaac ggtgtttgac
    10861 cgtctgccaa agctgcgtgg cgcacgctgg attgccgtag gtcgcctgga cgttaatacc
    10921 tgtggtctgc tgctgttcac taccgacggt gaactggcaa accgtttaat gcatccaagc
    10981 cgtgaagttg aacgtgaata cgccgtgcgc gtatttggtc aggttgacga cgcgaaactg
    11041 cgtgatttga gtcgtggcgt gcagctggaa gatggtccgg cagcctttaa aaccatcaag
    11101 ttcagcggcg gcgaagggat caaccagtgg tacaacgtta ctctgaccga aggccgtaac
    11161 cgtgaagttc gtcgtctgtg ggaagcggtt ggtgtgcagg taagccgcct gatccgtgtt
    11221 cgttacggtg atatcccact gccgaaaggt ctgccacgcg gtggctggac ggagctggac
    11281 ctcgcccaga ctaactatct gcgcgaactg gtggagctac cgccagaaac cagctctaaa
    11341 gtcgctgtag aaaaagaccg tcgtcgtatg aaggcgaatc agattcgtcg tgcggtgaaa
    11401 cgtcactgtc aggtgagcgg cggtcgtcgt tctggtggac gtaataataa cggttaattt
    11461 aagttaagta taaaataaag gccctg
//
LOCUS       DAZGQL010000134        11314 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.134, whole genome shotgun sequence.
ACCESSION   DAZGQL010000134 DAZGQL010000000
VERSION     DAZGQL010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11314
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.134"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..713
                     /gene="gspC"
                     /locus_tag="ACHKIR_004517"
     CDS             <1..713
                     /gene="gspC"
                     /locus_tag="ACHKIR_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HFO9992163.1"
                     /translation="NYFHTTIVKNGQIINQPTNAFQSEFSLAALWRNENHAGVKDANP
                     VAVNQETPKLSIALNGIVLTSNDETSFVLINEGSEQKRYSLNEALESAPGTFIRKINK
                     TSVVFETHGHYEKVTLHPGLPDIIKQPDSESQNVLADYIIATPIRDGEQIYGLRLNPR
                     KGLNAFTTSLLQPGDIALRINNLSLTHPDEVSQALSLLLTQQSAQFTIRRNGVPRLIN
                     VSVAELTGMNGQRHERTQ"
     gene            697..2649
                     /gene="gspD"
                     /locus_tag="ACHKIR_004518"
     CDS             697..2649
                     /gene="gspD"
                     /locus_tag="ACHKIR_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HFO9992164.1"
                     /translation="MKGLNKITCCLLAALLMPCAGHAENEQYGANFNNADIRQFVEIV
                     GQHLGKTILIDPSVQGTISVRSNDTFSQQEYYQFFLSILDLYGYSVITLDNGFLKVVR
                     SANVKTSPGMIADSSRPGVGDELVTRIVPLENVPARDLAPLLRQMMDAGSVGNVVHYE
                     PSNVLILTGRASTINKLIEVIKRVDVIGTEKQQIIHLEYASAEDLAEILNQLISESHG
                     KSQMPALLSAKIVADKRTNSLIISGPEKARQRITSLLKSLDVEESEEGNTRVYYLKYA
                     KATNLVEVLTGVSEKLKDEKGNERKPSSSGAMDNVAITADEQTNSLVITADQSVQEKL
                     ATVIARLDIRRAQVLVEAIIVEVQDGNGLNLGVQWANKNVGAQQFTNTGLPIFNAAQG
                     VADYKKNGGITSANPAWDMFSAYNGMAAGFFNGDWGVLLTALASNNKNDILATPSIVT
                     LDNKLASFNVGQDVPVLSGSQTTSGDNVFNTVERKTVGTKLKVTPQVNEGDAVLLEIE
                     QEVSSVDSSSNSTLGPTFNTRTIQNAVLVKTGETVVLGGLLDDFSKEQVSKVPLLGDI
                     PLVGQLFRYTSTERAKRNLMVFIRPTIIRDDDVYRSLSKEKYTRYRQEQQQRIDGKSK
                     ALVGSEDLPVLDENTFNSHTPAPSAR"
     gene            2659..4140
                     /gene="gspE"
                     /locus_tag="ACHKIR_004519"
     CDS             2659..4140
                     /gene="gspE"
                     /locus_tag="ACHKIR_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HFO9992165.1"
                     /translation="MRIHSPYPASWALAQRIGYLYSEGEIIYLADTPFERLLDIQRQV
                     GQCQTMTSLSQADFEARLEAVFHQNTGESQQIAQDIDQSVDLLSLSEEMPANEDLLNE
                     DSAAPVIRLINAILSEAIKETASDIHIETYEKTMSIRFRIDGVLRTILQPNKKLAALL
                     ISRIKVMARLDIAEKRIPQDGRISLRIGRRNIDVRVSTLPSIYGERAVLRLLDKNSLQ
                     LSLNNLGMTAADKQDLENLIQLPHGIILVTGPTGSGKSTTLYAILSALNTPGRNILTV
                     EDPVEYELEGIGQTQVNTRVDMSFARGLRAILRQDPDVVMVGEIRDTETAQIAVQASL
                     TGHLVLSTLHTNSASGAVTRLRDMGVESFLLSSSLAGIIAQRLVRRLCPQCRQFTPVS
                     PQQAQMFKYHQLAVTTIGTPVGCPHCHQSGYQGRMAIHEMMVVTPELRAAIHENVDEQ
                     ALERLVRQQHNALIKNGLQKVISGDTSWDEVMRVASATLENEA"
     gene            4137..5333
                     /gene="gspF"
                     /locus_tag="ACHKIR_004520"
     CDS             4137..5333
                     /gene="gspF"
                     /locus_tag="ACHKIR_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HFO9992166.1"
                     /translation="MNYRYRAMTQDGQKLQGIIDANDERQARLRLREEGLFLLDIRPQ
                     KSSGVKTRRPRISHSELTLFTRQLATLSAAALPLEESLAVIGQQSSNKRLADVLNQVR
                     SAILEGHPLSDALQHFPTLFDSLYRTLVKAGEKSGLLAPVLEKLADYNENRQKIRSKL
                     IQSLIYPCMLTAVAIVVVIILLTAVVPKITEQFVHMKQQLPLSTRILLGLSDTLQHTG
                     PTLLATVFIVAVGFWLWLKRGNNRHRFHAMLLRVALIGPLICAINSARYLRTLSILQS
                     SGVPLLDGMNLSTESLSNLEIRKRLANAAENVRQGNSIHLSLEQTAIFPPMMLYMVAS
                     GEKSGQLGTLMVRAADNQETLQQNRIALTLSIFEPALIITMALIVLFIVVSVLQPLLQ
                     LNSMIN"
     gene            5343..5780
                     /gene="gspG"
                     /locus_tag="ACHKIR_004521"
     CDS             5343..5780
                     /gene="gspG"
                     /locus_tag="ACHKIR_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HFO9992167.1"
                     /translation="MRATDKQRGFTLLEIMVVIVIIGVLASLVVPNLMGNKEKADKQK
                     AVSDIVALENALDMYKLDNHRYPTTNQGLESLVEAPTLPPLAANYNKEGYIKRLPADP
                     WGNDYVLVNPGEHGAYDLLSAGPDGEMGTEDDITNWGLSKKKK"
     gene            5788..6297
                     /gene="gspH"
                     /locus_tag="ACHKIR_004522"
     CDS             5788..6297
                     /gene="gspH"
                     /locus_tag="ACHKIR_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HFO9992168.1"
                     /translation="MNQQRGFTLLEMMLVLALVAITASVVLFTYGREDAANTRARETA
                     ARFTAALELAIDRATLSGQPVGIHFSDSAWRIMVPGKTPSAWRWVPLQEDAADESKND
                     WDEELSIHLQPFKPDDSNQPQVVILADGQITPFSLLMANAGTGEPLLTLVCSGSWPLD
                     QTLARDTRP"
     gene            6294..6671
                     /gene="gspI"
                     /locus_tag="ACHKIR_004523"
     CDS             6294..6671
                     /gene="gspI"
                     /locus_tag="ACHKIR_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HFO9992169.1"
                     /translation="MNKQSGMTLLEVLLAMSIFATVALALMSSMQGQRNAIERMRNET
                     LALWIADNQLKSQDTFNDENTSSSGKEIINGEEWNWRSDVHSSKDGTLLERTITVTLP
                     NGQKTALTRYQSIHDRSGQAQDD"
     gene            6664..7251
                     /gene="gspJ"
                     /locus_tag="ACHKIR_004524"
     CDS             6664..7251
                     /gene="gspJ"
                     /locus_tag="ACHKIR_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HFO9992170.1"
                     /translation="MINRQQGFTLLEVMAALAIFSMLGVLAFMVFSQASTLHQRSQKE
                     IQQFNQLQRTITILDNDLLQLVARRNRSTDKIMVLGEEAIFTTQSRDPQAPLNEAQTL
                     QTVHWYLRNHTLYRAVRTSVDGRKNQPAQAMLEHVESFLLESNSGESQELPLSVTLHL
                     KTQQYGALQRRFALPEQLAREESPAQTQAGNNNHE"
     gene            7244..8227
                     /gene="gspK"
                     /locus_tag="ACHKIR_004525"
     CDS             7244..8227
                     /gene="gspK"
                     /locus_tag="ACHKIR_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HFO9992171.1"
                     /translation="MNNEQRGVALLIVLMLLALMAALAADMTISFHGQLHRTRQVNHH
                     LQRQYDIELAEKLALASLTLDVKDNDRQTTLQQFWPQPQQLQLENGNTVKWQLRDAQH
                     CFNLNALAKISDAPLASPDFPAQVFSALLINAGIDRGNTDEIVQSIADYIDVDDSPRF
                     HGAEDNFYQSQTPPRHSANQMLFLTGELRQIKGITENIYQRLIPYVCVLPTTELSINL
                     NMLTENDIPLFRALFLNNITDADARVLLQKRPREGWLTTDAFLYWAQQDFSGVKPLVA
                     QVKGHLFPYSRYFTLSTESISDEQSQGWQSHIFFNRKQQSAQIYRRTLQLY"
     gene            8242..9405
                     /gene="gspL"
                     /locus_tag="ACHKIR_004526"
     CDS             8242..9405
                     /gene="gspL"
                     /locus_tag="ACHKIR_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HFO9992172.1"
                     /translation="MPESLMVIRSSSTLRKHWEWMTFSADSVSSVHTPTDDLPLESLA
                     DQPGAGNVHLLIPPEGLLYRSLTLPNAKYKLTAQTLQWLAEETLPDNTQDWHWTVVDK
                     QNESVEVIGIQSEKLSRYLERLHTAGLNVTRVLPDGCYLPWEVDSWTLVNQQTSWLIR
                     SAAHAFNELDEHWLQHLAAQFPPENMRCYGVAPHGVAATNPLIQHPEIPSLSLYSADI
                     AFQRYDMLHGVFRKQKTVSKSGKWLARLAVSCLVLAILSFVGSRGIALWQTLKIEDQL
                     QHQQQETWQRYFPQIKRTHNFRFYFKQQLAQQYPEAVPLLYHLQTLLLEHPELQLMEA
                     NYSQKQKSLTLKMSAKSEANIDRFCELTQSWLPMQKTEKDPVSGVWTVRSSGK"
     gene            9402..9863
                     /gene="gspM"
                     /locus_tag="ACHKIR_004527"
     CDS             9402..9863
                     /gene="gspM"
                     /locus_tag="ACHKIR_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HFO9992173.1"
                     /translation="MIKSWWAEKSTSEKQLVAALAVLSLGVFCWLGVIKPIDTYIAEH
                     QSHAQKIKKDIKWMQDQASTHGLLGHPALTQPIKNILLEEAKRENLAITLENGPDNTL
                     TIHPVTAPLENVSRWLTTAQITYGIVIEDLQFTLAGNEQITLKHLSFREQP"
     gene            9863..10540
                     /gene="gspO"
                     /locus_tag="ACHKIR_004528"
     CDS             9863..10540
                     /gene="gspO"
                     /locus_tag="ACHKIR_004528"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase GspO"
                     /protein_id="HFO9992174.1"
                     /translation="MTMLLPLFILVGFIAGYFVNAMAYHLSPLEDKTALTFRQVLVHF
                     RQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGALFLYFCFALTLSVIDFRTQLLPD
                     KLTLPLLWLGLVFNAQSGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
                     LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLG
                     YLAWISY"
     gene            complement(10569..11045)
                     /gene="bfr"
                     /locus_tag="ACHKIR_004529"
     CDS             complement(10569..11045)
                     /gene="bfr"
                     /locus_tag="ACHKIR_004529"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000675505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HFO9992175.1"
                     /translation="MKGDTKVINYLNKLLGNELVAINQYFLHARMFKNWGLKRLNDVE
                     YHESIDEMKHADRYIERILFLEGLPNLQDLGKLNIGEDVEEMLRSDLALELDGAKNLR
                     EAIGYADSVHDYVSRDMMIEILRDEEGHIDWLETELDLIQKMGLQNYLQAQIREES"
     gene            complement(11117..11311)
                     /gene="bfd"
                     /locus_tag="ACHKIR_004530"
     CDS             complement(11117..11311)
                     /gene="bfd"
                     /locus_tag="ACHKIR_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HFO9992176.1"
                     /translation="MYVCLCNGISDKKIRQAVRQFSPHSFQQLKKFIPVGNQCGKCVR
                     AAREVMEDELMQLPEFKESA"
BASE COUNT         2946 a         2914 c         2718 g         2736 t
ORIGIN      
        1 tgaattattt ccatacaact atagttaaaa atggacagat cattaatcaa ccaacaaacg
       61 catttcagtc tgagttcagc cttgccgctt tatggcgtaa cgaaaatcat gctggggtaa
      121 aagatgcaaa tcccgtggca gttaatcagg aaacacctaa gctctctata gcgcttaatg
      181 gcattgtact cacatcgaat gatgagactt cttttgtctt aattaatgag gggagcgaac
      241 aaaagcgata ctccctcaac gaagcccttg agtcagcgcc gggaacgttt attagaaaaa
      301 taaataaaac cagcgttgtg tttgaaactc acgggcatta cgaaaaagta actctccatc
      361 cagggctacc tgacatcatc aagcagcctg attcagaaag tcaaaatgta ctcgctgatt
      421 atattatcgc cacgccgata cgtgatggag aacagatata cgggcttcga ttgaaccctc
      481 gtaagggcct caatgcattt accaccagct tgctacaacc aggggatatc gccctcagga
      541 tcaataatct ttcgctgacc caccctgatg aggtttcaca ggcattaagt ttactgctaa
      601 ctcaacaaag tgctcagttt acaattcgtc gcaacggcgt accccgcttg ataaatgttt
      661 ccgtcgcgga acttacagga atgaatggac agcgtcatga aaggactcaa taaaatcacc
      721 tgctgcttgc tggcagcact actcatgcct tgtgcagggc acgctgagaa cgaacaatac
      781 ggcgctaact tcaataacgc cgatatccgc cagttcgtgg aaatagtggg tcagcatctt
      841 ggcaaaacga tcctgatcga cccttcggta cagggaacca tttccgtacg cagtaatgat
      901 acgtttagcc aacaggagta ctaccagttc tttttaagta ttcttgatct ttacggttat
      961 tccgtgatca cgctggacaa tggttttctg aaagtggtgc gctcagctaa tgtaaaaaca
     1021 tcgccaggga tgattgctga cagttctcgt ccaggcgtag gtgatgagtt ggtcacccga
     1081 atcgtaccgc ttgagaacgt tcctgctcgt gacctggctc ccctgctccg ccagatgatg
     1141 gatgcgggta gcgtcggtaa tgttgtgcat tatgaaccct ccaacgttct tattctgacc
     1201 ggtcgtgcct ccaccattaa taaactgatt gaagtcataa agcgcgttga tgtcatcggc
     1261 acagagaagc agcaaattat tcatctggaa tatgcgtcag cggaagatct cgccgaaatt
     1321 ttgaatcaat taatcagcga aagccacggt aaaagccaga tgcctgccct cctttccgcg
     1381 aagattgtgg cggataagcg aaccaactct cttatcatca gcgggccgga aaaagcacgc
     1441 cagcgcatca cttcattact gaaaagcctt gatgtagaag agagcgagga aggaaatact
     1501 cgggtttatt acctgaaata tgctaaagcc acgaatctgg tggaagtgct aaccggtgtt
     1561 tccgaaaagc tgaaagatga aaaagggaat gagcgtaagc cctcctcttc tggcgcgatg
     1621 gataacgtcg ccattaccgc cgatgaacag actaactctc tggtcattac cgctgaccag
     1681 tccgtccagg aaaaactcgc cacggtaatt gcgcgtctgg acattcgccg tgcacaggtg
     1741 ctggttgagg caatcatcgt tgaagttcag gatggaaatg gacttaacct cggcgtgcaa
     1801 tgggcgaata aaaacgttgg agcacagcaa tttaccaata ccggattacc gatttttaac
     1861 gctgcgcaag gtgtggctga ttataaaaag aatggtggga tcaccagcgc gaatcctgcc
     1921 tgggatatgt ttagcgccta caatggcatg gccgcaggct ttttcaatgg cgactggggc
     1981 gtattgttga ctgcgctggc cagtaacaat aaaaatgaca tcctcgccac cccgagcatc
     2041 gtaacgctgg ataataaact cgcgtccttc aacgttggtc aggatgtgcc ggtgctatct
     2101 ggctctcaga ccacttctgg ggataacgtc tttaatactg tcgaacgcaa aacggtgggc
     2161 acaaaactca aagtcactcc gcaggtcaat gaaggcgacg cggtactgct cgaaatagag
     2221 caggaggttt ccagcgttga ctcttcgtct aattcgacgc tcggcccgac atttaacacc
     2281 cgaactattc aaaatgccgt gctggtcaaa accggtgaaa cggtggtcct gggcggattg
     2341 ctggatgatt tttctaaaga gcaagtgtca aaggttcctc tgcttggcga tattccttta
     2401 gtggggcaac tcttccgcta tacttcgacc gagcgcgcta aacgcaacct gatggtattt
     2461 atccgcccaa cgattatccg tgacgatgat gtttatcgct cactgtcaaa agagaaatac
     2521 acccgctacc gtcaggagca acaacagcga atcgacggga aatcaaaagc gctggttggc
     2581 tcggaagatt tgccggtgct ggatgagaac acgttcaaca gccatacccc tgcgccctcg
     2641 gcacggtaag gcattcttat gagaattcac tcaccgtacc ccgccagttg ggcgctggca
     2701 caacgaattg gttatctcta ttcagagggc gagattattt atctcgccga tacgccattc
     2761 gaacggttac tcgatattca acgtcaggtt ggccagtgcc agaccatgac cagcttgtca
     2821 caggctgatt ttgaagcccg gctggaagcg gtgttccatc agaataccgg tgagtcgcaa
     2881 cagattgcgc aggatatcga tcaatccgtc gatcttctct cgctttcgga agagatgccc
     2941 gcaaatgaag atctcctgaa tgaagattca gcggcaccgg ttatccgctt gatcaatgcg
     3001 attttgagtg aggccatcaa agaaaccgcc tccgatatcc acattgaaac ctatgaaaaa
     3061 acaatgtcga tccgttttcg catcgacggt gttttgcgga caattttaca gccaaacaaa
     3121 aaactggcgg cactgcttat ctcccgaatt aaggtcatgg ctcgtcttga tatcgccgaa
     3181 aaacgtattc cacaggatgg acgtattagt ttgcgtatcg ggcgacgtaa catagatgtc
     3241 cgcgtatcca cactgccgtc catctatggt gaacgcgccg tgctccgtct gctggataaa
     3301 aacagcctcc agctttcatt gaacaacctg gggatgacgg cagcggataa acaggattta
     3361 gaaaatctca tccagcttcc acacggtatt atcctggtga ccggaccgac aggctccggt
     3421 aaaagcacca cgctctacgc catcctttcg gcgctgaata ctcccggccg caatattctg
     3481 acggtagaag atcccgtgga atatgagctg gaaggcattg ggcaaacgca ggtgaatacc
     3541 cgtgtggata tgtctttcgc tcgcggcctg cgcgccatac ttcgccagga cccggatgtc
     3601 gtcatggtgg gggaaattcg tgatacagaa accgcgcaga ttgcggttca ggcctcgctc
     3661 accggccatc tggtactctc aacactccac actaacagtg catcaggcgc agtgacccgg
     3721 ctccgcgaca tgggcgtcga atcattcctg ctttcgtctt ccctggcagg gattatcgcg
     3781 caacgtctgg ttcgtcgcct gtgtccgcaa tgccgacaat tcacgcccgt atcaccccaa
     3841 caagcgcaga tgtttaaata tcatcagctc gcggtgacaa caattggcac tcccgtaggc
     3901 tgccctcatt gccatcaatc cggctatcag gggcgcatgg cgatccacga aatgatggtg
     3961 gtgacgccgg aattacgggc cgctattcat gaaaatgtgg atgaacaagc actggagcga
     4021 ctggtccggc aacaacacaa tgccttaatc aaaaatggcc tgcaaaaagt gataagcggt
     4081 gacacctcct gggatgaggt tatgcgcgtc gccagtgcca cgctggagaa cgaagcatga
     4141 attatcgcta tcgcgccatg acccaggatg gtcaaaaatt gcaggggatc attgatgcta
     4201 acgatgaacg tcaggcacga ctgcggctgc gtgaagaagg gcttttcctg ctggatattc
     4261 gcccccaaaa aagttcggga gtaaaaacac gtcgcccgag gatcagccat agtgaactga
     4321 cgcttttcac ccggcagttg gcaaccttaa gcgcagcggc attacctctg gaagagagcc
     4381 ttgccgtaat cggtcaacaa agcagtaata aacgactggc tgatgtgtta aatcaggtac
     4441 gcagcgccat ccttgaaggg catccccttt ccgatgcgtt acaacatttt cccacgcttt
     4501 tcgattcgct ctaccgtacc ctggttaaag cgggcgaaaa gagcgggctg ctggccccgg
     4561 tgttggaaaa actggctgat tacaatgaaa atcggcagaa aatccgcagc aaactcattc
     4621 agtcgctgat ctacccctgc atgctgactg cggtggcgat tgtggtcgtg attattctcc
     4681 tcactgctgt cgtgcccaaa attaccgaac agttcgtgca tatgaagcag caactgccgc
     4741 tgagtacacg cattctttta ggtctgagcg acacgttgca acataccggc ccgacattac
     4801 tagcgacagt gtttattgtc gctgtaggtt tctggctctg gttaaaacgc ggcaataacc
     4861 gccaccgttt tcatgccatg ttgctgcgcg ttgcactcat cggcccgctg atttgcgcca
     4921 ttaacagcgc acgctatctc cgcactttaa gtattttgca atccagcggc gtccctctgc
     4981 tggatgggat gaatttgtcc accgaaagtc tcagcaacct cgaaatccgc aagcgtctgg
     5041 caaatgcggc agaaaacgtt cggcagggta acagcattca tctttcgctg gaacaaacgg
     5101 caatcttccc gccgatgatg ctctacatgg tggcctctgg cgaaaaaagc gggcagctcg
     5161 gcacgttaat ggtcagagca gcagataacc aggagacact ccaacaaaat cggatcgcct
     5221 taacgctctc catcttcgag ccagcactca ttattacgat ggcactgatc gtcctgttta
     5281 ttgtcgtctc ggtactccaa cctcttcttc aacttaactc aatgattaat taaggaaagt
     5341 atatgcgcgc aacggataag caacgcggtt ttacattact ggaaattatg gtggtgatcg
     5401 tcatcattgg cgtacttgcc agcctggtgg ttcccaacct gatgggcaat aaagaaaagg
     5461 cggataaaca aaaagccgtc agcgatattg tggcgctgga aaacgccctg gatatgtaca
     5521 agctcgacaa tcatcgctat ccaaccacaa atcagggact tgaatcctta gtcgaagcgc
     5581 cgacactgcc accgctggcc gcaaactata acaaggaagg ttatatcaag cgtctgcctg
     5641 ccgatccctg gggcaatgat tatgtcctcg ttaatcctgg tgaacatggt gcatacgatc
     5701 tgctttcagc agggcccgat ggtgaaatgg gaaccgagga cgacatcacc aactggggtt
     5761 tgagcaagaa gaaaaagtaa gtcagcgatg aatcagcaac gcgggtttac cctgctggag
     5821 atgatgctgg tgctggcgct ggtcgctatc acggcaagtg tggtgctctt cacatatggt
     5881 cgggaggatg ccgccaacac tcgtgcgcgc gaaacagcgg cgcggtttac cgctgcactt
     5941 gaattagcca tcgaccgcgc tacgctcagt ggtcagcctg tcggtatcca tttctctgat
     6001 tcagcatggc gcattatggt accgggcaaa acaccatcag cctggcgctg ggttccatta
     6061 caagaagatg ccgcagacga gagcaaaaat gactgggatg aagaactctc aattcatctt
     6121 cagccattta aacctgatga cagcaaccaa ccgcaggtgg tgattctggc cgacggacaa
     6181 atcacgccct tctctttgtt gatggctaac gcagggaccg gtgagccgct cctgacgtta
     6241 gtctgttcag gatcatggcc cctggatcaa acgctcgcca gagatacccg cccatgaaca
     6301 aacaatcagg gatgacactg cttgaagtct tactggcgat gagtattttc gccactgttg
     6361 cgctggcttt aatgagcagc atgcaagggc aacgaaatgc catcgagcgg atgcgtaatg
     6421 agacgctggc gctgtggatc gcagataacc agcttaagtc gcaagataca ttcaacgatg
     6481 aaaacacgtc cagttcaggc aaagagataa ttaatggcga agagtggaac tggcgcagcg
     6541 atgtccattc aagcaaagac ggcacgcttc tggaacgcac cataacggtg acgttaccca
     6601 acggtcagaa aaccgctctg actcgttatc agagcatcca tgatagatcc gggcaggcgc
     6661 aagatgatta atcgccaaca gggtttcaca ttgctggaag tcatggcggc actggcgatc
     6721 ttctcaatgc ttggcgtgct ggcgtttatg gttttttcgc aagcgtctac gctacatcaa
     6781 cgctcacaga aagaaatcca gcagttcaat caactgcaac gcaccatcac cattcttgat
     6841 aacgatctcc tgcaactggt cgctcgccga aatcggagta cggataaaat catggtgttg
     6901 ggtgaagagg ctatttttac cacgcaaagt cgcgatccgc aggccccgct caatgaagcg
     6961 caaaccttac aaacggttca ctggtatctg cgaaatcata cgctttaccg tgctgttcgt
     7021 acttctgtgg atggcaggaa gaatcaacct gcccaggcga tgctggagca tgtcgagagc
     7081 tttcttctgg aaagtaacag cggggaaagc caggagcttc ctctctcagt cacgttacat
     7141 ctgaagactc agcaatatgg ggcgttacag cgccgttttg cattacctga gcagttagcg
     7201 agagaagagt cgccagctca gacgcaggcc gggaataata accatgaata atgaacaacg
     7261 cggcgtagca ctgctcattg tactcatgtt actggcgctc atggctgctc tggctgcaga
     7321 catgacgatc agttttcacg gccagcttca tcgaacccgc caggtaaacc atcatttgca
     7381 acggcaatat gatattgagc tggcggagaa acttgcgctt gccagcctca ccctggacgt
     7441 taaagataac gacagacaaa ccacgttgca gcagttctgg ccacaaccgc aacagttgca
     7501 gctggaaaat ggcaatacag tgaagtggca gttacgcgat gcccagcact gttttaatct
     7561 caatgcctta gcaaaaatct ctgacgcccc gctggcttcc cctgatttcc ccgcccaggt
     7621 attcagtgcc ctgctgatta acgccggtat cgatcgcgga aacaccgatg agatcgtgca
     7681 atcaattgct gactatattg atgtcgatga ctcaccacgc tttcacggcg cagaagataa
     7741 cttttaccaa agccagacgc cgccccggca tagtgccaat cagatgcttt ttctgaccgg
     7801 cgaattgcgt cagataaaag gcataaccga aaacatctat cagcggctta tcccgtatgt
     7861 ctgcgttctc ccaaccacgg aacttagcat caatcttaat atgcttacgg agaacgatat
     7921 tccgctcttc agagcgttat tcctcaataa catcacggat gctgacgctc gggtcttatt
     7981 acaaaaaagg cccagggaag gctggctaac cacggatgca tttctttact gggcgcagca
     8041 ggatttctcc ggcgtgaagc ctctggtagc tcaggtgaaa gggcatctct ttccctatag
     8101 ccgctatttt acgctgagta cggaaagtat cagcgacgaa caatcccagg gatggcaaag
     8161 ccatattttc ttcaaccgta aacagcagag cgcccaaatc tatcggcgta cgctgcaact
     8221 ctactaatca taaggatcgt gatgcctgaa tcgttgatgg tcatacgttc ttcctcgacg
     8281 ctgcgtaagc attgggaatg gatgacgttc tccgcagata gcgtatcttc ggtacatacg
     8341 cccacggacg atcttccgct ggagtcgctg gccgatcagc ccggtgccgg aaatgttcat
     8401 cttttgatcc caccagaggg ccttttatat cgttccctca cgctgcccaa cgccaaatac
     8461 aaactcaccg cacaaacatt gcaatggctg gcagaggaga cgttacccga caacacccag
     8521 gactggcact ggacggttgt cgataaacaa aacgaaagtg tcgaggtgat aggcattcag
     8581 tcggagaaac tcagtcgcta tcttgagcgt ttacatactg ccggactcaa cgtgacgcgg
     8641 gttttgccag atggctgtta tcttccctgg gaagtagata gctggactct ggttaaccag
     8701 caaacaagct ggcttattcg cagtgccgca catgccttca atgaactgga tgaacactgg
     8761 ttacagcatc ttgctgccca gtttccgcct gagaatatgc gttgttatgg tgtggcacct
     8821 cacggcgttg cagcgaccaa tccacttatc cagcatcctg aaataccatc actgagtttg
     8881 tattctgccg atattgcatt ccagcgctat gacatgctgc atggtgtttt tcgtaaacag
     8941 aaaactgtaa gcaaatcggg aaaatggctc gcccgcctgg cagtaagctg tctcgttctc
     9001 gccattcttt cttttgttgg cagccggggc atcgcgctct ggcaaacgct taagattgaa
     9061 gatcaacttc agcatcaaca gcaagaaacc tggcaacgtt atttcccgca gatcaaacgc
     9121 acccacaact ttcgttttta ctttaagcag caactcgctc agcaatatcc tgaagctgtg
     9181 ccgttgctct atcatttaca aacacttctg ctggaacacc ctgaactgca gttaatggag
     9241 gcgaactata gccagaagca aaagtcgctg accttaaaaa tgagtgctaa gagtgaagcc
     9301 aatatcgatc gtttctgtga attaacacag tcctggcttc cgatgcaaaa aacagaaaaa
     9361 gatccggtca gtggtgtatg gacagtaagg agctcaggga aatgattaaa tcatggtggg
     9421 cagaaaaatc cacatcggaa aaacagctcg ttgcggcgct ggcggttctc tcgctcggcg
     9481 ttttttgttg gcttggggtg ataaaaccga tagataccta cattgcagag catcagtccc
     9541 acgcgcaaaa aattaagaag gacatcaaat ggatgcagga tcaagcgagc acccatggtt
     9601 tactcggtca tcctgcgctt acgcagccca tcaaaaatat ccttctcgag gaagcaaaac
     9661 gtgagaattt ggctatcacg ctggaaaacg gtccggacaa tactctgaca attcatcctg
     9721 tcacagctcc cctcgaaaac gtctcccgct ggctcactac ggcacagatt acatacggta
     9781 ttgttattga agaccttcag tttactcttg ccggaaacga acagatcacc ctcaagcatt
     9841 tgtccttcag ggagcaacca taatgacaat gctactgccg ctcttcattc tggttggttt
     9901 tattgcaggt tattttgtta atgccatggc ctatcacctc tcgccgttgg aggataaaac
     9961 ggcgttaacg tttcgccagg tattggttca tttcaggcaa aaaaaatatg cctggcatga
    10021 tacagtgccc ctgatacttt gcgttgctgc tgctatcgcc tgcgccctgg cacctttcac
    10081 gcccatcgtg actggcgcac tctttctcta tttctgtttc gcactcacgc tcagtgttat
    10141 tgattttcgc actcagctcc tgcccgacaa actcacctta ccgctgctct ggcttggctt
    10201 ggtatttaat gcgcagtctg gattaattga tttacatgat gcggtttacg gcgcggtagc
    10261 tggctatggg gtgctgtggt gtgtttactg gggcgtctgg ttagtttgtc acaaagaggg
    10321 attgggctac ggtgatttca agctactggc cgctgcaggc gcgtggtgtg gctggcaaac
    10381 gttgccaatg atactgctaa ttgcctcact gggtggcatt ggttacgcca tcgtttcaca
    10441 acttctgcaa cgccggacta taaccactat cgcattcggc ccgtggcttg cgctcggcag
    10501 catgataaac ctggggtatc tggcctggat ctcgtattaa ccgggagggt tctccctccc
    10561 gacacggctc aactttcttc gcggatctgt gcttgcagat aattttgcag gcccatcttc
    10621 tgaatcagat caagttccgt ttccagccag tcgatatggc cttcttcatc acgcaaaatt
    10681 tctatcatca tatcgcggct gacgtaatca tgaacgctat cggcataacc aattgcctca
    10741 cgcaaattct tcgcgccatc cagctcaagt gccagatcag aacgcagcat ttcctcaaca
    10801 tcctcaccaa tgttcagttt gcccaggtcc tgtaaatttg gaagaccttc cagaaaaaga
    10861 atgcgctcaa tataacgatc ggcgtgtttc atctcatcaa tggattcatg atactccaca
    10921 tcattgagac gtttgagacc ccagttttta aacatccggg catggagaaa gtactgattg
    10981 attgcgacaa gctcatttcc caacagtttg ttgagataat ttataacctt agtatcacct
    11041 ttcatttttt gactccttcg cttccactct ttgaagcgta gaacggctta cgagtaagtc
    11101 aaaaaacaca ctccgtttat gcggactcct taaactccgg caactgcatt aattcatcct
    11161 ccatcacttc acgcgcggcc cggacacatt taccgcactg attgccgacc ggaataaatt
    11221 tttttaattg ctggaacgag tgaggggaaa actggcgcac agcctgacga atttttttat
    11281 cgctgatacc attacaaaga caaacgtaca taga
//
LOCUS       DAZGQL010000135        11181 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.135, whole genome shotgun sequence.
ACCESSION   DAZGQL010000135 DAZGQL010000000
VERSION     DAZGQL010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11181
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.135"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..291
                     /locus_tag="ACHKIR_004531"
     CDS             <1..291
                     /locus_tag="ACHKIR_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HFO9992177.1"
                     /translation="RDKALAWVEKNIKVPLTEPQKAGIASFCPYNIGPGKCLPSTFYK
                     RLNAGDRKGACESIRWWIKDGGRDCRTRSNNCYGQVIRRDQESALACWGIDQ"
     gene            288..755
                     /locus_tag="ACHKIR_004532"
     CDS             288..755
                     /locus_tag="ACHKIR_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001520736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFO9992178.1"
                     /translation="MRRVTAIIYALVICIIVCLLWAVNHYRDNAITYKAQRDKKAREL
                     ELANATITDMQQRQRDVAALDARYSRELADARAENETLRADVAAGRKRLRINATCPGS
                     VREAPTTSGVDNATGPRLADTVERDYFTLRERLMMMQKQLEGAQEYIRTQCLK"
     gene            1058..1864
                     /locus_tag="ACHKIR_004533"
     CDS             1058..1864
                     /locus_tag="ACHKIR_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HFO9992179.1"
                     /translation="MAKLDWKKLEQAFRREHAETGITLLDWCRKKKINYNTARTRIKM
                     GKIDHEIDHKTDHEIDHDISDEEPSNDACSDDEKCAKNSEKNCANSAETKRIRGSRLL
                     PPSNAFSQRNTHAVRHRGYAKYLEADNLMDDASDMVLFDELVFTRARALSVTKALKGM
                     FADLEEATDVETRVALYDKILKAEQALDRNIARIESIERSLLTLDVLAETAPKLRADR
                     ERINAARDKLRAETDILTSQRRGVITPVSDIVSSLHEMSNSGRLDDIPEE"
     gene            1845..3551
                     /locus_tag="ACHKIR_004534"
     CDS             1845..3551
                     /locus_tag="ACHKIR_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HFO9992180.1"
                     /translation="MTFRKNKTRCDEPAEMTETEQRLFIMTKLSNPWWRLNHLYKIQN
                     EKGELVTFRMRPAQRQLFRNMHNKNIILKARQLGFSTAIDIYLLDQALFIPHLKCGIV
                     AQDKQAASEIFRTKIAVPFDHLPDWLRASFTIVERRSGASGGYILFGHGSSIQVATSF
                     RSGTVQRLHISEHGKICAKYPAKAKELRTGTLNAVSDECIIFDESTAEGVGGDFYEMS
                     NRAQESTASGLLLTAQDYKFHFYAWWQDPKYSARVPESGLKLSREKMTYFSAVEKAMN
                     ITLTDEQKQWYISKETEQREEMKQEFPSTPQEAFLTSGRRVFSAESTLQAESFCSPPM
                     IVYDIEPVTGAKTKAQSLREGNKNELQRTLMNYLLVWELPDPDEEYVCGADTAEGLEH
                     GDRSSLDVVKRSNGEQVAHWFGHLDAELFAHLISQVCRMYNNAFVGPERNNHGHAVIL
                     KLRELYPTRYIYNEQHLDQAYDDDTPRLGWLTTRQSKPVLTEGMKTLLNNGISGIRWS
                     GTLSEMNTYVYDAKGSMNAQEGCFDDQLMSYMIAQEMRARMPVRVKQKTDKRRTTHWM
                     AH"
     gene            3551..5695
                     /locus_tag="ACHKIR_004535"
     CDS             3551..5695
                     /locus_tag="ACHKIR_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFO9992181.1"
                     /translation="MKNEINTTAMKNDHGSTPRFSQRQLLSLCSDIDSQPLWRDAANK
                     ACAYYDGDQLAPEVIQVLKDRGQPMTIHNLIAPTVDGVLGMEAKTRTDLIVMSDDPND
                     ETEKLAEAINAEFADACRLGNMNKARSDAYAEQIKAGLSWVEVRRNSDPFGPEFRVST
                     VSRNEVFWDWLSRESDLSDCRWLMRRRWMDTDEAKATFPGMAQVIDYAIDDWRGFVDT
                     TITEGQPSPLMSAWEEYQSWDRRENEWLQRERRRVLLQVVYYRTFERLPVIELSNGRV
                     VAFDKNNLMQAVAVASGRVQVKVGRVSRIREAWFVGPHFIVDRPCSAPQGMFPLVPFW
                     GYRKDKTGEPYGLISRAIPAQDEVNFRRIKLTWLLQAKRVIMDEDATQLSDSDLMEQI
                     ERPDGIIKLNPARKNQKSVADVFRVEQDFQVASQQFQVMQESEKLIQDTMGVYSAFLG
                     QDSGATSGVAISNLVEQGATTLAEINDNYQFACQQVGRLLLAYLLDDLKKRRNHAVVI
                     NRDDRQRRQTIVLNAEGDNGELTNDISRLNTHIALAPVQQTPAFKAQLAQRMSEVIQG
                     LPPQVQAVVLDLWVNLLDVPQKQEFVERIRAALGTPKSPDEMTPEEQEVAAQQQALQQ
                     QQAELQMREMAGRVAKLEADAARAHAAAQRDNASAQREVALTQGQRYVDALNQAHTAE
                     IITGVQNMEQEQDVLQQQMLYTLQQRMNEMSL"
     gene            5853..6860
                     /locus_tag="ACHKIR_004536"
     CDS             5853..6860
                     /locus_tag="ACHKIR_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992182.1"
                     /translation="MDFEFTGEETPEQLEKMLEGLGDVDIDSHAQDVVTEDTTEKHAD
                     EEAQTQTGDNNVAPTPDASVEQTQDVKEPEAKGVLTRDGKHVIPYEVLEAERSGKQRA
                     EQEAALLRGQIAEEKRRVELLTSQIHQAGMKPTPLPENEKISDEQIARIREMYPEIGD
                     AVASLIRKNNYLQSRVQQSTQQAEGNGGEDLSPVLDAMNAVPVLKTWQESDPDRFSVA
                     VSIDGKLQNDPAWKDKTLTERFAEVARRTQVAFGEVSESSADNQADKTDIRKTAEEKV
                     KTAEQEQAVPASPSDLGTTASVGTGDNFERLLGASHSEAEAIMRGMTNAEIDALLEKL
                     G"
     gene            6884..8098
                     /locus_tag="ACHKIR_004537"
     CDS             6884..8098
                     /locus_tag="ACHKIR_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N4-gp56 family major capsid protein"
                     /protein_id="HFO9992183.1"
                     /translation="MTTVTSAQANKLYQVALFTAANRNRSMVNILTEQQEAPKAVSPD
                     KKSTKQTSAGAPVVRITDLNKQAGDEVTFSIMHKLSKRPTMGDERVEGRGEDLSHADF
                     SLKINQGRHLVDAGGRMSQQRTKFNLASSARTLLGTYFNDLQDQCAIVHLAGARGDFV
                     ADDTILPTAEHPEFKKIMINDVLPPTHDRHFFGGDATSFEQIEAADIFSIGLVDNLSL
                     FIDEMAHPLQPVRLSGDELHGEDPYYVLYVTPRQWNDWYTSTSGKDWNQMMVRAVNRA
                     KGFNHPLFKGECAMWRNILVRKYAGMPIRFYQGSKVLVSENNLTATTKEVAAATNIDR
                     AMLLGAQALANAYGQKAGGHFNMVEKKTDMDNRTEIAISWINGLKKIRFPEKSGKMQD
                     HGVIAVDTAVKL"
     gene            8154..8543
                     /locus_tag="ACHKIR_004538"
     CDS             8154..8543
                     /locus_tag="ACHKIR_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992184.1"
                     /translation="MAKTILAPSLSERVYTGTHGNESVAEGVFTVNAAEADSVIHLLS
                     LPVGIRINSLQLVSTGGLGTATVSIKSGEHALIDNSEAVSAKFARYVPVEPYTTQRDG
                     ELVTVTIKTAAATGTLNVLLRYTVVGY"
     gene            8593..9054
                     /locus_tag="ACHKIR_004539"
     CDS             8593..9054
                     /locus_tag="ACHKIR_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992185.1"
                     /translation="MSEKIAVVYIGPKPVKKDTITGSRTLFPRLEPVHVDSAMAWQLL
                     GFPDVWVRHEELDDVLKKQQQNEQLRQAQQAQESVLAARAEAENSFVVSVNGQEVDLS
                     KLTSARLATLCEAEELDIHKDPKETAEAFRIRVREAFRRRVAETEQHGGTE"
     gene            9038..9601
                     /locus_tag="ACHKIR_004540"
     CDS             9038..9601
                     /locus_tag="ACHKIR_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992186.1"
                     /translation="MAELSDFLPYVRRHISGPLNIMMTDALSMAAVAFSRQSLVCRRE
                     VTVVPVAGKEIVLPYDKDDEECVHIIRISDDNHELFVGRDVDISSGRSLRFACSPGEV
                     SVLYAVAPKAGRSQIPDELLTWPEEVAAGALERLFMQTGVSWSDPLRAQYFSVQFSEG
                     IRRAYRHTLATSPYSSYRNPVRRQRFY"
     gene            9601..10251
                     /locus_tag="ACHKIR_004541"
     CDS             9601..10251
                     /locus_tag="ACHKIR_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6682 family protein"
                     /protein_id="HFO9992187.1"
                     /translation="MTTITEIIGRVNTQLVDPMMVRWPLQELCDYYNDAVRAVILARP
                     DAGASLETISCVPGARQVLPDGVIQLLDVICLSDGSAVRPLSREVLDAQYPEWPTMKG
                     IPECFISNDLSPRVFWLFPVPDKEISIDAVVSRIPEAVYVLTQDDDTPVPLEEAYVNP
                     LVDWMLFRAFSKDAAGGAESGLAAQHYQSFVEQLGIKQGADSALSARKKVFNGGGV"
     gene            10248..>11181
                     /locus_tag="ACHKIR_004542"
     CDS             10248..>11181
                     /locus_tag="ACHKIR_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HFO9992188.1"
                     /translation="MSVVVSGTLKSPDGEAISGANITLTALTVSPDALSGTSASAVTR
                     EGGYYGMTMDPGEYAVSVTVKGKTAVYGRVRIEGTESTVTLNMLLRRSLVEVSIPGEL
                     LTDFRQIQNNVADDLATIRRLNEDTATKNTQATQSKESAAASAKSASDSAKTATSRAA
                     EAGQKASEAAEAATRAITAAGNAEESSTRAGESEKAAGADAEKARLHAEKARLAQESA
                     GEILKRAEAATVSAEEARRMAENARGPRGPQGETGPKGDVGPKGETGPVGSQGPAGPK
                     GERGDVGAQGAVGPAGPRGEKGEQGERGPQGIPGL"
BASE COUNT         2876 a         2614 c         3211 g         2480 t
ORIGIN      
        1 cgtgataagg cgctggcatg ggtggagaaa aacatcaaag tgccactgac cgaaccccag
       61 aaagcgggta ttgcgtcatt ctgcccgtat aacattggcc ccggtaagtg tctcccgtcg
      121 acgttttata agcggcttaa tgccggtgat cgtaaaggtg cctgtgagtc gattcgctgg
      181 tggattaagg acggtgggcg tgattgccgc acacgttcaa ataactgcta cggacaggtt
      241 attcgtcgtg accaggaaag cgcattagct tgttggggga tagatcagtg agaagagtaa
      301 ccgcgattat ttacgctctg gttatctgca tcatcgtctg tctgttatgg gctgttaatc
      361 attaccgcga taacgccatc acctacaaag cgcagcgcga taaaaaagcc agagagctgg
      421 agctggcaaa cgcaaccatt actgacatgc agcagcgcca gcgtgatgtt gctgcgcttg
      481 atgccagata ctcaagggaa ttagccgatg cgagagctga aaatgaaact ctgcgcgctg
      541 atgttgccgc tggtcgtaag cgcctgcgga tcaacgccac ctgtccaggc tccgtgcgtg
      601 aagcccccac cacctccggc gtggataatg caaccggccc ccgactggca gacacagttg
      661 aacgggatta tttcaccctc agagagcggt tgatgatgat gcagaagcag ctggaagggg
      721 cacaggaata tatccgcact cagtgcctga aataagtttt gctgatgcgc cgtatcgtcg
      781 ccgtattccc gtattaacag cgactgcagc cctgacgggt acctcctgcg tgagtgtgcg
      841 gggataatca aaaacgatac acaccgggtt tccctcattt ttcaggagac gtgagcattt
      901 acccgcgaag ccgcctgtcc ggtgcggtgg tggaagaaac cggataaaac aacctcattg
      961 tacaaatatc gatcaagtat ggcgctgctg tgtgaattct gaaaaatcac agcggtcatt
     1021 atgcgtcagt ttttaacaga ggacgtcaga aagtgacatg gcaaagctgg actggaaaaa
     1081 gctggagcag gcattccgac gcgaacatgc cgaaacggga ataacattac tggactggtg
     1141 ccggaagaaa aagattaatt acaacaccgc cagaacccgt ataaaaatgg gcaaaatcga
     1201 tcatgaaatt gatcataaaa ccgatcatga aatcgatcat gacatctcag atgaagaacc
     1261 cagcaatgac gcgtgttccg acgatgaaaa atgcgcaaaa aactctgaaa aaaactgcgc
     1321 aaattcggca gaaacgaaac ggattcgtgg ttcccgactt ttacctcctt caaacgcttt
     1381 ttctcagcga aacacccacg ccgtaagaca ccgtggatat gcgaagtatc ttgaggcaga
     1441 taacctcatg gatgatgcgt ccgacatggt gctgttcgat gaactggtgt tcacccgggc
     1501 ccgcgcactt tcagtaacta aggcacttaa agggatgttc gccgacctgg aagaggcaac
     1561 tgacgtggaa acccgcgttg ctctttacga caaaatactc aaagctgaac aggcccttga
     1621 ccggaatatt gcccgtatcg agtcaattga acgctcattg ctgacgctgg acgtcctggc
     1681 tgagacagca ccaaaacttc gtgctgaccg ggaaagaatc aacgccgcca gagataaact
     1741 cagagcggaa accgatattc tgaccagcca gcgccggggg gttattacgc cagtcagtga
     1801 catcgtgtca tcgctgcatg aaatgagtaa ttcggggaga ctggatgaca ttccggaaga
     1861 ataaaacgcg atgtgatgaa cctgcagaaa tgactgagac cgaacaacgt ctgttcatta
     1921 tgacaaaact gagcaatccc tggtggcgcc tcaatcatct ctacaaaata cagaacgaaa
     1981 aaggtgaact ggtcaccttc agaatgcgac cggcgcaacg tcagttgttt cgtaacatgc
     2041 ataataaaaa cattatcctg aaagcgcgcc agctgggatt ttccacagcc attgatattt
     2101 atcttctcga ccaggcatta ttcattccgc atctcaaatg cgggatcgtc gctcaggata
     2161 aacaggctgc cagtgaaatt ttccgcacaa aaattgctgt accgtttgat catctccctg
     2221 actggctgag agcctcattc accatcgttg aacgtcgtag cggtgccagc ggtggctata
     2281 tcctgtttgg tcacggctcg agtattcagg tggcaacctc atttcgctca ggtacggtgc
     2341 agcgcctgca tatctcagag cacggcaaaa tttgcgcgaa atatccggct aaggcgaaag
     2401 agctgcgaac cggtacgctt aatgccgtct ctgatgaatg cattattttt gatgaatcca
     2461 cggctgaagg cgtgggtggt gatttttacg agatgagtaa ccgtgcacag gagagcactg
     2521 catcaggctt attgctgacg gcacaggatt ataaattcca tttttacgca tggtggcagg
     2581 atcctaaata cagcgccaga gtgcctgaaa gcgggctgaa gctgtcacgg gaaaaaatga
     2641 cgtatttttc tgcggttgag aaggcaatga acatcacgct taccgatgag cagaagcagt
     2701 ggtacatcag taaggaaact gaacagcgtg aggaaatgaa gcaggagttt ccctcaacgc
     2761 cacaggaggc gtttctgacg tccggacgac gtgtgttcag tgccgaaagt acattgcagg
     2821 cagaatcatt ctgttcgcca ccgatgattg tttatgacat tgaacctgtt acaggagcga
     2881 agactaaagc tcagtctctg cgtgaaggaa ataaaaacga gttgcagcgg acgctgatga
     2941 attatctgct ggtatgggaa ctgccggatc cggatgaaga gtatgtttgt ggggcagata
     3001 ctgccgaagg gctggagcac ggagaccgct catcgctgga tgttgtcaaa cgcagtaatg
     3061 gcgagcaggt ggctcactgg ttcgggcatc tcgatgctga actttttgct catctcattt
     3121 cgcaggtctg tcgtatgtat aacaacgcgt ttgtggggcc ggagcgtaat aatcacggac
     3181 atgcagttat cctgaaactc cgggaactct atccgacacg ttatatctac aacgaacagc
     3241 atcttgacca ggcatatgac gacgatacgc cccgccttgg ctggctgaca acccgtcaga
     3301 gcaaacctgt tctgaccgaa ggaatgaaaa cgctcctgaa taatggaata tcagggatcc
     3361 gctggtcagg cacattatcg gaaatgaaca cctacgttta tgacgcgaaa ggctccatga
     3421 atgcacagga aggctgcttt gatgatcagc tcatgagcta catgattgcc caggagatgc
     3481 gcgccagaat gccggtgagg gtaaaacaga aaacggataa acgcagaacc acacactgga
     3541 tggcacactg atgaaaaatg aaattaacac cacagcgatg aaaaacgatc atggatccac
     3601 gccgcgtttt tctcagcgtc agttactgtc tctctgttct gatattgaca gtcagcctct
     3661 ctggcgtgat gccgcaaaca aggcctgtgc gtattatgat ggcgaccagc tggcaccgga
     3721 agttatccag gtactgaaag atcgcggtca gcccatgacc atccataacc tcattgcccc
     3781 cacggtagat ggtgtactgg gaatggaggc aaaaacaaga acggacctga tagtgatgtc
     3841 agacgatccg aacgatgaaa cagagaaact ggcagaggcc attaatgcgg agtttgctga
     3901 tgcgtgccgt cttggcaaca tgaataaggc ccgctccgat gcctatgcgg aacaaatcaa
     3961 ggcggggctc agttgggtgg aggtcaggcg caacagcgat ccgttcggac ctgaattcag
     4021 ggtatccaca gtcagcagaa atgaggtttt ctgggactgg ctcagccggg aatccgacct
     4081 gagtgactgc cggtggctga tgcgtcgccg ctggatggat accgatgagg caaaagccac
     4141 gttcccggga atggctcagg ttatcgatta tgctattgat gactggcgtg gttttgtcga
     4201 taccacgatt actgaaggcc agcccagtcc gttgatgagt gcatgggaag agtatcagtc
     4261 atgggatcgc agggagaatg aatggcttca gcgtgaacgc cgtcgtgtgc tgcttcaggt
     4321 ggtttattac cgtacattcg aacgtcttcc ggtgattgaa ctcagtaatg gacgggtggt
     4381 ggcttttgat aaaaataatc tgatgcaggc ggtggctgtg gcatccgggc gggttcaggt
     4441 gaaagtcggg cgggtaagtc gtattcgtga agcctggttt gtgggcccac actttattgt
     4501 ggatcgcccc tgtagtgctc cgcaggggat gtttccgctg gttcctttct ggggataccg
     4561 aaaggataaa accggggagc catacgggtt aatttcccgc gccattccgg cacaggatga
     4621 ggtgaatttt cgtcgtatca agctgacctg gctgcttcag gccaaacgcg tgattatgga
     4681 cgaggatgcc acccagttgt cagacagcga cctgatggag cagatcgaac gtccggatgg
     4741 cattattaaa ctgaatccgg cccgaaaaaa tcagaaaagt gtcgcagatg tttttcgggt
     4801 tgagcaggat tttcaggttg ccagccagca gtttcaggtc atgcaggaat cggaaaaact
     4861 tatccaggat accatgggag tgtattccgc atttctcggg caggattcag gtgcgacgtc
     4921 aggcgtggct atcagtaacc tggtggagca gggggccaca actcttgcgg aaatcaacga
     4981 taactaccag tttgcctgcc agcaggtggg aagactgttg ctggcttatc ttctcgatga
     5041 cctgaaaaaa cgccgtaatc atgcagtggt gattaatcgc gatgatcgcc agcgtcgcca
     5101 gaccattgtc ctcaatgctg aaggtgataa tggtgaactg accaatgata tttcaaggtt
     5161 aaatacacat attgcgctgg cgcctgttca gcagacaccg gcgtttaagg cacagcttgc
     5221 acagagaatg tcagaggtta ttcaggggct gccgcctcag gtgcaggctg ttgtgctcga
     5281 cctgtgggtt aatcttctgg atgtgccgca gaaacaggag tttgttgagc gtattcgtgc
     5341 tgcgctgggg acgccaaaat caccggatga aatgacgccg gaagaacagg aagtagcggc
     5401 acaacaacag gcacttcagc aacaacaggc agaactccag atgcgcgaga tggctggcag
     5461 agtggcaaaa ctggaagctg acgccgccag ggcacatgca gctgcacaac gggataatgc
     5521 cagtgcacag cgggaagtcg ccctgacaca ggggcagcgt tatgtggatg cgcttaacca
     5581 ggcacatacg gcagaaatca ttaccggcgt acagaatatg gaacaggagc aggacgttct
     5641 tcagcaacag atgctgtata cgttacaaca gcggatgaat gaaatgtcgc tctgaaaact
     5701 ctggcttcaa ctgaaccccg tcatcgtacg gggttttttg tttccggagg taagcgttcc
     5761 gggagcggtg cgcttattcg cgggggcagc gataagcctt atttactcaa ccattcggat
     5821 ctgtccgata aacagaccat gcggagttat ttatggattt tgaatttacg ggtgaagaaa
     5881 ccccggaaca actggaaaaa atgctggaag gacttgggga tgtggatatt gacagtcacg
     5941 cacaggacgt cgtgacggaa gataccacgg aaaaacatgc ggatgaggaa gcacagactc
     6001 agacgggcga taacaatgtg gcaccgacgc cggatgccag tgtggagcag acgcaggacg
     6061 tgaaggagcc ggaagcgaag ggggtgctca cccgcgacgg taaacacgtc attccctatg
     6121 aagtccttga ggctgaacgt tccggtaagc aacgggccga acaggaagcc gcacttcttc
     6181 gtgggcagat agctgaagaa aaacgcaggg tggaactgct gacgtctcag atccaccagg
     6241 ccggtatgaa gcccacaccg ttaccggaaa acgaaaaaat ttctgatgag cagattgccc
     6301 gtatcaggga gatgtatccg gaaattggtg acgcggtggc ttcgctcatc cgtaaaaata
     6361 actatctcca gtcccgtgtt cagcaatcaa cacagcaggc agaaggtaat ggtggtgagg
     6421 atttatcacc ggttcttgat gcgatgaatg ccgtgccggt gctgaaaacg tggcaggagt
     6481 ccgatccgga tcgcttctcg gttgctgtat ccatcgacgg gaagctccag aatgaccccg
     6541 catggaaaga caaaacgctc actgaacgtt tcgctgaagt ggcccgtcgt acgcaggttg
     6601 ctttcggtga agtcagtgag tcgtctgctg acaaccaggc agacaaaacg gatatccgga
     6661 aaacggcgga agaaaaagtg aagaccgctg aacaggagca ggcagtacct gcttccccgt
     6721 cagatttagg caccacggcc tccgtcggaa ccggtgataa ttttgaacgg ttacttggtg
     6781 cttctcattc agaggctgag gcgattatgc gtggtatgac gaatgctgaa atagacgcac
     6841 ttctggagaa gctcgggtaa cttactgaag gagtactgaa gtaatgacga ctgtaacatc
     6901 agcccaggcg aataagctgt atcaggtggc gctttttact gctgccaacc gcaaccgctc
     6961 gatggttaat atccttactg aacagcagga agcgccaaaa gcggtttcgc cggacaagaa
     7021 aagcacgaag cagaccagcg caggtgcgcc ggttgtccgt atcacagacc ttaacaaaca
     7081 ggccggtgat gaagtgacct tcagcatcat gcacaaactc tcaaaacgtc cgacgatggg
     7141 agatgagcgt gttgaaggtc gtggtgagga tctcagccat gctgacttct ccctgaaaat
     7201 caatcaggga cgtcacctgg tggatgcagg cggacgtatg agtcagcagc gcacgaagtt
     7261 taacctggca tcctctgcca gaacgcttct ggggacgtac tttaatgacc tgcaggacca
     7321 gtgtgcgata gtgcatcttg ccggagcacg tggtgatttt gttgctgacg acactattct
     7381 gccgacagcg gagcaccctg aattcaaaaa aatcatgatc aacgatgtac tgcctccgac
     7441 acatgaccgt cacttttttg gcggtgatgc gacaagcttt gagcagattg aagcggcaga
     7501 tattttttct attggcctgg tggacaatct ctccctgttc attgacgaaa tggcgcatcc
     7561 gttacagccg gttcgtctgt ccggtgatga acttcacgga gaagatccat attacgtcct
     7621 gtacgtcacg ccgcgtcagt ggaatgactg gtacacctcg acgtccggta aggactggaa
     7681 ccagatgatg gttcgtgccg tgaaccgtgc aaaaggtttt aatcatccgc tgttcaaagg
     7741 tgaatgtgcg atgtggcgca atatcctggt tcgtaagtat gcgggtatgc cgatccgttt
     7801 ctatcagggg tcaaaggttc tggtatcaga gaataacctg acggcaacca cgaaagaggt
     7861 cgctgctgca accaatattg accgcgccat gttactgggg gctcaggcgc tggcaaatgc
     7921 ttacggtcag aaggcgggcg gtcacttcaa catggttgag aagaaaacgg atatggataa
     7981 ccgtactgag atagcaatca gctggatcaa cggtctgaaa aaaatccgtt tccccgagaa
     8041 gagcggcaag atgcaggatc acggcgtgat tgccgttgat acagcagtga agctctgatt
     8101 ttttcctttc cccatgccgg gttttcgccc ggctttttca ggagtcatta attatggcaa
     8161 agactatcct tgccccgtca ctgagtgaac gggtctatac gggtacgcac ggtaatgagt
     8221 cggtggcaga aggcgtattt acggtgaatg ctgcggaagc ggacagtgtt attcatcttc
     8281 tctcactgcc agtgggcatc cgtatcaact cactccagct ggtttcaacg ggtggtctgg
     8341 gtactgcaac cgtcagcatt aagtccggtg agcatgctct catcgataac agcgaagctg
     8401 tttctgcaaa atttgccaga tatgtgccag tggagccgta caccacacag cgtgacgggg
     8461 agctggttac tgtcaccatt aagactgccg ctgcaaccgg caccctgaat gttctgctgc
     8521 gttataccgt ggtgggatac tgattaaaac cttccggccc gcgtcatgcg ggctttttat
     8581 ccggggaatt atatgagtga gaaaattgcc gttgtctata tcggcccaaa acccgtgaaa
     8641 aaggacacca ttaccggaag ccgcacattg ttcccacgtc ttgagccggt gcatgttgac
     8701 agtgcgatgg cctggcaact gctggggttt ccggatgtct gggttcgtca tgaagagctt
     8761 gatgatgttc tgaaaaagca acaacagaat gagcagttgc ggcaggcaca gcaggcgcag
     8821 gaaagcgtgc ttgctgcgcg ggcagaagcg gagaacagtt ttgttgtttc tgttaacggg
     8881 caggaggtgg atttaagtaa gctcacctca gcacggctgg cgacgctgtg tgaggcagaa
     8941 gagctggata ttcacaaaga cccgaaagaa acggctgagg cattccgtat ccgggtgcgt
     9001 gaggcatttc gccgtcgtgt tgcggagact gaacagcatg gcggaactga gtgatttttt
     9061 accgtatgtc cgtcgtcata tcagcggtcc actgaacatt atgatgacgg atgctctgtc
     9121 aatggctgcc gtggcattca gccgccagtc gttggtgtgc cgtcgggagg ttactgttgt
     9181 accggtggca ggaaaagaaa tcgtgcttcc gtatgacaaa gatgatgagg agtgcgttca
     9241 tatcatccgt atctctgacg ataatcatga gctttttgtc ggtcgggatg tggatatcag
     9301 ctccggacgc tccctgcgat ttgcctgttc tcccggtgag gtgagcgtgc tttatgccgt
     9361 cgctccgaaa gccggacgca gccagatacc ggatgaactc ctcacatggc ctgaagaagt
     9421 ggctgcgggg gcacttgagc ggttgttcat gcagactggt gtttcatggt cagatccgtt
     9481 acgcgcacag tatttttctg tgcagttttc tgaggggatc cgtcgggcat atcgtcatac
     9541 actggcgaca agcccgtact cttcataccg caaccctgta cgcaggcaga ggttttactg
     9601 atgacgacga ttacagaaat catcggacgt gtgaatacac aactggttga cccgatgatg
     9661 gttcgctggc ccctgcagga attgtgcgat tattacaatg atgctgtgag ggcagtgatt
     9721 ctggcgagac cggatgctgg cgcaagcctg gaaacaatca gttgtgttcc tggcgcccgt
     9781 caggttttgc ccgatggtgt aatacaactt cttgacgtga tatgcctcag tgatggtagt
     9841 gcagtcagac cattatcccg ggaggtgctg gatgcgcagt atcctgagtg gcccacaatg
     9901 aagggcattc ctgaatgttt tatcagcaac gacctgtccc cgcgcgtatt ctggctgttt
     9961 cctgtccctg acaaagagat aagtattgat gcagtggtaa gccggatacc ggaggcagtg
    10021 tatgttctga cgcaggacga tgatacgcca gttccactgg aagaggctta tgttaaccca
    10081 ctggtggact ggatgttgtt tcgtgctttc agtaaggatg ctgccggtgg tgcagaatcg
    10141 gggctggctg cgcagcatta tcagagtttt gttgagcaac ttgggatcaa acagggggca
    10201 gacagtgcat tgtctgcccg taaaaaagtg tttaacggag gtggagtgtg agtgttgttg
    10261 tttcggggac gctgaaatct cctgatggtg aggcgatatc aggagcaaat attaccctga
    10321 cggcgctgac agtttcaccg gatgcgctca gcggcaccag tgcgtcggca gtgacccgtg
    10381 aaggtggata ttacggaatg acgatggatc cgggggagta tgcggtttcg gtgacggtga
    10441 aagggaagac tgctgtctac ggacgtgtgc gtattgaggg gaccgaaagt acggtgacgc
    10501 tcaatatgct gttacgccgc agtcttgttg aggtgagcat acccggagag ctgctgacag
    10561 atttccggca gatacagaat aatgtggctg atgaccttgc tactattcgt cgcctgaatg
    10621 aagacacggc gacaaaaaac actcaggcca cacagtcaaa agaaagtgca gcagccagtg
    10681 cgaagagtgc atctgacagt gcaaagacgg caaccagcag ggcggctgaa gccggacaaa
    10741 aagccagtga agctgcagaa gcagcgaccc gtgcaatcac agcagcgggg aatgcagagg
    10801 aaagctcgac ccgtgccgga gagtctgaaa aagccgccgg agctgatgca gaaaaagcca
    10861 gactgcatgc tgaaaaggcc aggctggcgc aggagagcgc cggagagatc cttaagcggg
    10921 cagaggctgc cactgtcagt gctgaagagg ccagacgtat ggctgagaat gcacgggggc
    10981 cccgggggcc tcagggagaa actggtccga agggggatgt cggtcctaaa ggcgaaacag
    11041 gtccagtggg ctctcaaggg cccgcagggc cgaaaggtga gcgtggtgac gttggtgctc
    11101 agggggcagt agggcccgct ggtccgcggg gtgagaaggg cgaacagggg gagcgaggac
    11161 cgcagggaat accaggcctg a
//
LOCUS       DAZGQL010000136        11084 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.136, whole genome shotgun sequence.
ACCESSION   DAZGQL010000136 DAZGQL010000000
VERSION     DAZGQL010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11084
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.136"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(457..1218)
                     /locus_tag="ACHKIR_004543"
     CDS             complement(457..1218)
                     /locus_tag="ACHKIR_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03747 family integrating conjugative element
                     membrane protein"
                     /protein_id="HFO9992189.1"
                     /translation="MAEPRREVNRAAQPSPPRQHGLLWNLLWGWPWMLVGILFSSLLL
                     SLLVEYIGIAFFWPEQGAAHSEAVMKTELGWLSSEFTRSLLLSTPSVTVTEWIATAYQ
                     WLFVETGFLSWLKQQNAGAVVGNSVSQYSEDVGRWLSHYLCEYLMATVYVTVVTLIRV
                     TILVLSVPLFVMVILIAVVEGLGRRDLRRYGAAYESSFVYHHAKKSIKPAIYIPCIFY
                     LSWPTSVYPNLLIWPAAMLLGVAITVTMASFKKYL"
     gene            complement(1211..3307)
                     /gene="traD"
                     /locus_tag="ACHKIR_004544"
     CDS             complement(1211..3307)
                     /gene="traD"
                     /locus_tag="ACHKIR_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001510361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HFO9992190.1"
                     /translation="MSNRYVIEALLRPAVELNTAVVAATAAGVCVTAPWAVALAPSVS
                     YVTAAGFGVLAVVRFRQGMKIIRYRRNLRRLPRYVMSSRQIPVSRQRLFLGRGFRWTQ
                     KHTQRLQDTLRPEVAHYLQPGSLYRTARWLEMKTEHSLPWIGQLLRRDSPLNPVRPLP
                     PVGGNPALHGIEPDEQDVTLALGERVGHTIVYGTTRVGKTRLAELLVTQDIRRGDVTI
                     VFDPKGDADLLLRVWAEAHRAGRGDELYIFHLGWPEISARYNAVGRFGRVSEVASRVA
                     GQLSGEGNSAAFREFAWRFVNIVARALVALGERPDYTLIMRYVNNIADLYIRYAGKVI
                     SEQMPELENAILNNMNVLGEADVPRTMQNQPDAVRIWAIEVALSSEAGKKLYDPILDG
                     LRSAVRYDRTYFDKIVASLLPLLEKLTTGKTAELLAPDYLDMSDTRPVFDWEQVIRKK
                     AVVYIGLDALSDSEVASAVGNSMFADLVSVAGHIYKHGMNAGLPGGNGTKNPVNLHCD
                     EFNELMGDEFIPLINKGGGAGMQVTAYTQTSSDIEARTGSAAKTAQVQGNFNNMIMLR
                     VRENRTAELLTTQLSQVEIYTKTLVSGHQDTADVTTGQDFTSSTQDRVGTVKVPLLEP
                     ADIVTLPKGQAFALLEGGQLWKIRMPLPATDADDALMPESIAKIAEAMRRNYHTGEGW
                     WNETPSVNMPEGEQNG"
     gene            complement(3304..4092)
                     /locus_tag="ACHKIR_004545"
     CDS             complement(3304..4092)
                     /locus_tag="ACHKIR_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000675383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992191.1"
                     /translation="MKGAFTERIQANAPAWAHEQLVAWDTAEFAAMSDFITEHYWTGQ
                     GSINVYRIVGTDHPQYAGMSWLELLERGKRMDINIPLLEKNPGYYTQAEQQHAGMGFV
                     STDGIHWYVSADGNHRSCLARFLFHLQGEGRTQLHNVAQSIYHTDQAFRSACREIHNL
                     AEPLSRHDVYLRLQIRRQCVSREDLACWKVDRFRTEALLTVDDVHAGGHDGPLVYKAL
                     LLNAADAWREVMVLQRRLEALSASPEKALPRSWWRRLLQRGTRS"
     gene            complement(4089..4571)
                     /locus_tag="ACHKIR_004546"
     CDS             complement(4089..4571)
                     /locus_tag="ACHKIR_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992192.1"
                     /translation="MIMTLYRFVMLLLRLLIVWPSMLAGCLVMLYVWNNLSGGEAGMT
                     QRYATESSRWRDAPEGHVMVQKCSSVISVSGMYVDEQECPRTAISFGEAVAEDIRELK
                     MQAVVLLLGALFMEGVSSVILFRGCGSAAVSGEGRGGPAVMPWPDGIGISKVQERRAE
                     "
     gene            complement(4568..5161)
                     /locus_tag="ACHKIR_004547"
     CDS             complement(4568..5161)
                     /locus_tag="ACHKIR_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000982739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFO9992193.1"
                     /translation="MMPLSATSAALSASPVLTACLVLSLLAVVLGPALWRSRRKKACV
                     RTRRHQRYRATASRVMKRLNELQGDARRLKYLRKISPYVFEELLLLAFEQQGMPVIRN
                     PSYSGDGGLDGQVVINGERWLIQAKRYGRTIRPAHIDAFGELLRRKGCRGFFIHTGRT
                     GNISRARLTLWPDIELISGQRLLQLLNGTLLSNKGRS"
     gene            complement(5158..5676)
                     /locus_tag="ACHKIR_004548"
     CDS             complement(5158..5676)
                     /locus_tag="ACHKIR_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrating conjugative element protein"
                     /protein_id="HFO9992194.1"
                     /translation="MKRERQFYVLSLLLFCTTAGAELKVIADLGGRDASPFFEAINRQ
                     PPVPASVVPSPSFPQGEAAMLPVSTPEMSPGEVVARRLQLPGIGALFLVGDDAHSRTW
                     LQMNAAVLRERKAAGMVVNVADESSLRVLRELAPGVVMVPASGSVLARRLQISHYPVL
                     ITDNGLSQVPAP"
     gene            complement(5673..6353)
                     /locus_tag="ACHKIR_004549"
     CDS             complement(5673..6353)
                     /locus_tag="ACHKIR_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HFO9992195.1"
                     /translation="MARGGILTVLLMVACSGVCRAAVQNVPDGYVRVARAHGIPAELL
                     YAVSLTETSMSPEAMSGVLKQRRRDFRQPDVARPWPWTINVAGKGYRYATRLEAWQAL
                     QVFLKHYPAKRIDVGIAQVNLGWNGHRFPSTWAAFDPYTSLNTAAVILQECRRRHPVS
                     WLQVAGCYHHPAGGAPAARYTAVVRRHLARLHRDNFLNPSDVHVAATAMLTPSPRYIW
                     TEPQEEQK"
     gene            complement(6332..7051)
                     /locus_tag="ACHKIR_004550"
     CDS             complement(6332..7051)
                     /locus_tag="ACHKIR_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016191739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03759 family integrating conjugative element
                     protein"
                     /protein_id="HFO9992196.1"
                     /translation="MKRKILYLLALCPVLAQSAGQTNMSSEAQRTTGTVVTQSTTSGL
                     QQQAGQWGVSEEDYRRYQSLMSGPRGIQSPGLDPLTVLGIEARTPAERRVWAEKWVRA
                     EYERTEKELAFQQEVNAAWKRLYPSILPVSMGKTGALTGDTGGRLALFVKAKECGTCD
                     ARLASVLATGRQVDIYLVDSQGKDEILRQWAHAHSIPVEKVRSRHITLNHDAGRWLRF
                     GEGKMPVVLQQGADGWRVAAF"
     gene            complement(7055..7804)
                     /locus_tag="ACHKIR_004551"
     CDS             complement(7055..7804)
                     /locus_tag="ACHKIR_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992197.1"
                     /translation="MSEKQPQIPEGASGMSPERRKSRFPGVLILSVTVGLVMGAVVYG
                     VLMLMTGLDSRITTLEGQRREMLSASELAPLRMKLTETEKQLKDMEKRLAVLEQQPVL
                     TASWKEQLENLPEMLEQIESAREKLSLRLDTLSGLVEKMQKVPESPSQPDSRKAGMKK
                     TEEKKPAPVTSSRKSRAVHTVRQAPFVLTGTELRGGSTRAAVAPRGYTSLSQVALLGE
                     GESVAGWTLVYAGHGEATFRVNGRQTVLRVE"
     gene            complement(7801..8400)
                     /locus_tag="ACHKIR_004552"
     CDS             complement(7801..8400)
                     /locus_tag="ACHKIR_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilL N-terminal domain-containing protein"
                     /protein_id="HFO9992198.1"
                     /translation="MKKRFILSGLTVCLLMAGCQSTQKLQQRDAAMPVPSVTVNRNVQ
                     PASPDVYSQAPEVVRYDRYLLVSTEPQAVQRDPLSQVIDIRIPSSLKPTVADAMRYAL
                     KQSGYTLCATGPANGVLYRQPLPAVQYQPGPLRLRTALQLMAGPAWQLEVDDVQRVVC
                     HSLRDGYQLPVSQLAPVPVTVPFVGREPGVKATGGFLKK"
     gene            complement(8586..9578)
                     /locus_tag="ACHKIR_004553"
     CDS             complement(8586..9578)
                     /locus_tag="ACHKIR_004553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFO9992199.1"
                     /translation="MNENTPTTPTPHDAAFRAMMECPSVARDFLKGALPPEQLNRCDM
                     NTLQLEPTTFVDPDLRQFACDVLWSMKTTDGRDGYIYTLTEHQSKADKYMALRMMRYV
                     LAVIHRHLKAHACAPVVIPVLFYHGVPSPYPYSMNWPDCLDDPAFGRELYGEGRPPRV
                     IDVGLLDDEGIRRYKQMAALMLMMKLRQKKVDLMVHLDFLSQLIQIQETHTQVVTLFN
                     YMVKECDSASPEFIRAMAERLPRHEDDLMTIAERLELAGIEKGRQEGIEKGRLEGIEK
                     GRQEGIEKGRQEEREKALQENYASARRMQQMGISSEKIKQILQFSDDDLRNALR"
     gene            complement(9627..10187)
                     /locus_tag="ACHKIR_004554"
     CDS             complement(9627..10187)
                     /locus_tag="ACHKIR_004554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004115562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO9992200.1"
                     /translation="MASRGVNKVILIGHLGQDPEIRYFPNGGGAVANLTLATTDTWRD
                     KQSGEMKEHTEWHRVVIYGKLAEIAGEYLRKGSQVYIEGQLRTRKWFDQQNGVDRYST
                     EVVVSVNGTLQMLGSPRQQQGGGNVSSAPAQGGWGQPPQNAQQSTGKNGQASSGSGGM
                     PPMDFDDDIPFMGRGYGVERRIIHVI"
     gene            complement(10203..10406)
                     /locus_tag="ACHKIR_004555"
     CDS             complement(10203..10406)
                     /locus_tag="ACHKIR_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992201.1"
                     /translation="MKPTSGILAAKAGYTLPLQILSSARGYYIGTQCDEGPVSRESEE
                     YFRNYEQAVRALENGIWTQRECV"
     gene            complement(10497..10964)
                     /locus_tag="ACHKIR_004556"
     CDS             complement(10497..10964)
                     /locus_tag="ACHKIR_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016151303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STY4534 family ICE replication protein"
                     /protein_id="HFO9992202.1"
                     /translation="MTANTSSANAKTEYFNLTLKGLGYLSNIRQVNHQNGTFLSCVVN
                     ALSGPTDSPSYVRFDVTVAGKEATSLIARCQKAVDEDRKVLIGFTMSNPSTDIFTLNR
                     GEHAGEQRVSLKARLIKVEWIKIGQDMVYKTEKSESIPPQNGNTPREYAENSF"
BASE COUNT         2553 a         3389 c         2638 g         2504 t
ORIGIN      
        1 aataaactat gttctggggg ctgtcagcat agccagtttc tgcatctcgc tgttccagat
       61 tctgttcaaa taaatttatt ccagcctgaa tgatttattc ctgtttatta aaacacggca
      121 tcactactgt ttcccgttaa tgacacctca ttaacaattt aaagaggaaa cacatcagca
      181 tggaatttct ccgtactgcc cttccgttct gggccctgtt ccttctgacc tgggtttgtg
      241 ttgtgtattt tttcatttat gaagaacctc gttgcccccc gtggatctat gacgtcatca
      301 gggccatcac tctgttcatt attttcgtca acattgttgt cattccggcg ctgtcgttac
      361 tctgacactg acgcgcactg accgtaaggg cgtggcttgt ctgccgacac cctgccacaa
      421 aacaagtttt gcatcagggt gtccggcctc tgtggattat aaatatttct tgaaggatgc
      481 catggtcacg gtgatggcca cccccagcag catggccgcc ggccatatca gcaggttcgg
      541 atacaccgac gtcggccagg acaggtaaaa aatgcaggga atatatatcg ccggtttaat
      601 agactttttt gcatggtgat aaacaaagct ggactcatac gcagcaccat accggcgcag
      661 atcacgacgc cccagtccct ccacaaccgc gatcagtatc accatcacaa acagcggaac
      721 agacaacacc aggatcgtca cccgtatcag tgtgaccacc gtgacatata ctgtggccat
      781 caggtactca cacagataat ggctcagcca gcgccccaca tcctcactgt actgtgacac
      841 actgttaccc accacggcac cggcattttg ctgcttcagc cacgacagaa aacctgtttc
      901 cacaaacagc cactgatacg ccgttgctat ccattctgtc accgttaccg atggtgtcga
      961 cagcagcaga ctgcgggtga attccgaaga cagccacccc agttctgtct tcatcactgc
     1021 ctcactgtgt gctgccccct gctccggcca gaaaaaagca atcccgatat actcaaccag
     1081 caggctcagc agcagggagg aaaacaggat cccgaccagc atccacggcc agccccacaa
     1141 cagattccag agcagtccgt gctgacgtgg cggagagggt tgtgctgctc tgttcacctc
     1201 acgacggggt tcagccattc tgctctcctt ccggcatgtt cacggaggga gtctcattcc
     1261 accatccctc tccggtgtgg taattacgcc gcatggcctc tgcaattttt gcaatactct
     1321 ccggcatcag cgcgtcatct gcatcagttg caggcagggg catacggatt ttccacaact
     1381 gtcccccttc cagcagagca aaggcctgtc ctttcggcag ggtcacgata tctgccggct
     1441 ccagcaaagg caccttcaca gtaccaacac gatcctgggt actggatgtg aagtcctggc
     1501 cggtcgtgac atctgcggta tcctgatgac cggaaaccag cgttttggta taaatttcca
     1561 cctgggacaa ctgggtcgtc agtaactcgg ccgtccggtt ctccctgacc cgcagcataa
     1621 tcatgttatt aaagttaccc tgcacctggg ccgttttggc agcactaccg gtacgggcct
     1681 caatatcaga agaggtctgc gtgtaagccg tcacctgcat accggcaccg cctcccttgt
     1741 tgattaacgg aatgaactca tcgcccatca gttcattaaa ctcatcacaa tgcaggttaa
     1801 ccggattttt ggtaccattc cctcccggaa gaccggcatt cattccgtgt ttgtagatat
     1861 gcccggcgac actcaccaga tccgcaaaca tggagttacc cacggcactg gccacctcac
     1921 tgtcactcag cgcatcaaga ccaatgtaca ccaccgcctt tttacgtatc acctgctccc
     1981 agtcaaacac gggacgggta tcgctcatat ccaggtagtc aggtgccagc agttctgccg
     2041 tctttcctgt ggtcagtttt tccagcaacg gcaacaggga tgccacaatt ttgtcaaaat
     2101 aggtacgatc ataacgtacc gcgcttcgca gaccatccag tatcggatca tagagtttct
     2161 ttccggcttc actgctgaga gccacctcaa tggcccagat acgcacagca tccggctggt
     2221 tctgcatggt ccggggaaca tccgcttccc ccagtacatt catgttattc agaatggcgt
     2281 tttccagttc cggcatctgt tcgctgatca ccttaccagc ataacggata taaaggtcgg
     2341 caatattatt cacataacgc ataatcagcg tgtaatctgg gcgctccccc agtgccacca
     2401 gtgcacgggc aacaatgttg acaaaccgcc atgcaaactc acggaaggcc gcactgttac
     2461 cttcaccgga taactgaccg gccacacggg aagccacctc cgacacacga ccaaaacgcc
     2521 ccacggcgtt ataacgcgca gagatttccg gccagcccag atgaaagata taaagctcat
     2581 cgcccctgcc ggcacggtgt gcttcagccc agacacgcag caacagatcc gcatcccctt
     2641 tgggatcaaa gacaatggtg acatctcccc gacgaatatc ctgtgtgacc agcaactccg
     2701 ccagccgggt tttcccgaca cgggtggtgc catagacaat ggtgtgcccc acacgttcac
     2761 ccagtgccag cgtcacatcc tgctcatccg gctcaatacc atgcagggca ggatttcctc
     2821 ccaccggcgg caggggacgg acgggattca gcggcgagtc acgacgaaga agctgcccaa
     2881 tccagggaag actgtgctcc gttttcattt caagccagcg cgctgtccgg tacagactgc
     2941 ccggctgtag atagtgtgca acctccggtc tcagcgtgtc ctgaaggcgc tgggtgtgct
     3001 tctgtgtcca gcggaagccg cgtcccagaa acagccgctg acggctgacc ggaatttgcc
     3061 gggaactcat cacataacgg ggcagccggc gcagattgcg acggtaacgg atgatcttca
     3121 ttccctgccg gaaacggacc actgccagaa cgccaaagcc ggcggctgtg acatagctga
     3181 ccgaaggcgc gagcgccacc gcccagggcg ctgtcacaca gacaccggcc gccgttgctg
     3241 ccaccacggc ggtattcagt tccacagccg gacgcagcag agcctcaatc acataacggt
     3301 tactcatgac cgggtacctc tctgtaaaag acgccgccac catgagcgcg gcaacgcttt
     3361 ctccggtgat gctgacagtg cctccagtct gcgctgtaac accatcacct cccgccatgc
     3421 gtctgccgca ttcagcagta atgccttata caccagtggc ccgtcatgtc cgccggcatg
     3481 cacatcatcc actgtcaaca gcgcctctgt tctgaaacgg tccactttcc agcaggccag
     3541 gtcttcgcgg gaaacacact gccgccggat ctgcagacga agatacacat catgccggga
     3601 caacggttct gccaggttgt gaatttcccg acaggcagac cggaacgcct ggtctgtgtg
     3661 ataaatactc tgggccacat tgtgaagctg tgtgcgcccc tccccctgca ggtggaacag
     3721 aaagcgggcc agacagctgc ggtgattacc gtcagcactg acataccagt gaatgccatc
     3781 cgtgctgaca aaacccatcc cggcatgctg ttgctctgcc tgcgtgtaat atcccggatt
     3841 cttttcaagt aacgggatat tgatgtccat gcgtttacca cgctccagca gctccagcca
     3901 gctcattccg gcatactgtg gatggtcagt ccccacaatg cggtacacat tgatactgcc
     3961 ctgtcccgtc cagtagtgtt ccgtaataaa atccgacatt gccgcaaact ctgcggtatc
     4021 ccatgcaacc agttgttcat gtgcccaggc cggagcattc gcctgtatcc gctctgtaaa
     4081 tgcccccttc attcagctct tctctcctgc acttttgaga taccgatacc gtcaggccac
     4141 ggcataacag ccgggccccc cctgccttcc ccggatacgg ctgcagaacc acaaccacga
     4201 aacagaatga ctgacgagac gccttccata aacaaagcgc ccagcaacag aaccactgcc
     4261 tgcattttca gttcacgaat atcctcagca acagcctccc cgaaagaaat cgctgttcgc
     4321 ggacattcct gctcatcgac atacatacct gagacggata tgacggagga acatttttgt
     4381 accatcacat gcccttcagg cgcatcacgc cagcgggatg actccgtcgc ataacgctgt
     4441 gtcatgcctg cctcaccacc actcagattg ttccagacat acagcattac cagacatcct
     4501 gccagcattg acggccagac aatcagaagc cggagcagca gcataacaaa ccggtacagt
     4561 gtcataatca tgatcttcct ttattactca gtaaggtgcc attcagcagt tgcaacagac
     4621 gttgtccact gatcagctca atatccggcc acagtgtcag ccgtgcccgg ctgatgttcc
     4681 ccgtacgccc cgtatggata aaaaagcccc tgcatccctt ccggcgcagc agttcgccaa
     4741 aagcatcaat atgtgccgga cgaatggttc ttccgtaacg tttagcctgt atcagccagc
     4801 gttcaccgtt aatcaccacc tgaccatcaa gaccaccatc gccgctgtat gaggggttac
     4861 ggataaccgg catcccctgt tgctcaaaag ccagcagcaa cagttcctca aacacatacg
     4921 gactgatttt gcgcagatat ttcagccgcc gggcatcacc ctgcaattcg ttcagccgct
     4981 tcattacccg tgaggccgtg gcacgataac gctgatgccg gcgtgttctc acacacgctt
     5041 tttttcgccg gctgcgccac agtgccggcc ccaggacaac agccagcagg ctgagaacca
     5101 ggcaggctgt cagtaccgga gaagcagaga gcgcagcaga agtggcagac agcggcatca
     5161 tggtgcaggt acctgtgaca ggccgttgtc cgttatcagc accgggtaat gacttatctg
     5221 taacctgcgg gccagtacag aaccggatgc cggaaccatc accacccccg gtgccagctc
     5281 acgcagtacg cgcagactgc tctcatccgc cacattcact accattcccg ccgccttccg
     5341 ctccctgagt actgctgcat tcatctgcag ccatgtgcgg gaatgggcat catcgcccac
     5401 aagaaaaagt gcaccgatgc ccggtaactg aagccggcgc gccacaacct ctccgggtga
     5461 catttcaggc gtactgaccg gtaacattgc cgcctctccc tgcggaaaag agggtgatgg
     5521 cacaactgaa gcgggaaccg gtggctgccg gtttatcgcc tcaaaaaagg ggctggcatc
     5581 cctgccgccc aggtctgcaa tcactttcag ttctgcgcca gctgttgtac agaacagcag
     5641 cagactcagt acataaaact gtctttctcg tttcatttct gctcctcctg tggttcagtc
     5701 cagatataac ggggggatgg tgtcagcatg gcggtcgctg ccacatgcac atcagacgga
     5761 ttcaggaaat tatcccggtg gagccgggca agatgccgtc tgaccacggc ggtataacgt
     5821 gcggccggcg caccacctgc cgggtggtga taacacccgg ccacctgcag ccagctgacc
     5881 ggatggcgac gccggcattc ctgcagaatg accgctgccg tattcagact ggtgtaagga
     5941 tcaaatgccg cccatgtgga cgggaaacga tgtccattcc agcccagatt gacctgtgct
     6001 atcccgacat caatacgttt tgccggataa tgtttcagga acacctgcaa tgcctgccag
     6061 gcctccagac gtgtggcata acggtaccct tttcctgcca catttattgt ccagggccag
     6121 ggtcgggcca cgtccggctg acgaaaatca cgacgccgtt gtttcagtac tccggacatc
     6181 gcttccggag acatggacgt ttccgtcagg gaaacggcat acaaaagctc tgcgggaata
     6241 ccatgggcac gggccacccg cacatacccg tccggtacgt tctgaacggc agcacgacag
     6301 acaccactgc aggccaccat cagcagaacc gtcagaatgc cgccacgcgc catccgtcag
     6361 ccccctgctg cagtaccacc ggcatttttc cttcaccaaa acgcagccag cgtccggcat
     6421 catgattcag ggtgatatga cggctgcgaa ctttctccac aggtatgctg tgcgcatgcg
     6481 cccactgacg cagaatctca tcctttccct gactgtcgac aaggtagata tcaacctgcc
     6541 gtcccgtggc cagtacggag gcaagacggg catcacaggt gccacattcc ttcgccttca
     6601 caaacagcgc cagccgtccc cccgtatctc cggtaagtgc tcctgttttt cccatactga
     6661 caggcaagat actgggatac aggcgcttcc aggccgcatt cacctcctgc tgaaacgcca
     6721 gctccttctc tgttcgttca tattcagccc tgacccactt ttcggcccag acacgtcgct
     6781 ctgccggcgt tctggcctct ataccgagta ccgtcagcgg atcaagtccg ggagactgta
     6841 tcccccgggg gccggacatc agtgactgat aacgccggta atcctcctca ctcacccccc
     6901 actgcccggc ctgctgctga agtccggatg tggtactctg cgtcaccacc gtaccggtcg
     6961 tccgttgcgc ctctgacgac atattcgtct gcccggcgga ctgagccaga acggggcaca
     7021 gtgccagcag atacagtatt tttcgcttca tcatctactc caccctgagc accgtctgac
     7081 gaccattcac ccggaacgtt gcttcaccat ggccggcata cacaagcgtc cacccggcca
     7141 cactctctcc ttcacccagc agagccacct gagagaggct ggtatacccc cggggagcca
     7201 cggctgcacg ggtactgcca ccgcgcagct ctgtcccggt cagcacaaaa ggagcctgcc
     7261 gaactgtatg aactgcgcgg gatttacggg aggacgtcac cggagcgggc tttttctcct
     7321 ccgttttttt catccccgct ttcctgctgt ccggctgtga cggtgactcc ggtactttct
     7381 gcattttctc caccagaccg ctgagtgtat ccagtctcag ggaaagcttt tcccgggcag
     7441 actcaatctg ctccagcatc tccggcagat tctccagttg ttccttccag gatgctgtca
     7501 gcacgggctg ctgctccagg actgccaggc gcttttccat atccttcagt tgcttctccg
     7561 tctctgtcag tttcattctc aggggcgcga gttctgacgc agaaagcatt tctcttctct
     7621 ggccttccag ggtcgtgatc cggctgtcaa gaccggtcat aagcatcagt acgccatata
     7681 ccactgcccc catgaccagc cccacagtga cagacagtat caacacgccg gggaaacgac
     7741 tttttcttcg ctccggcgac ataccagacg caccctccgg tatctgcggc tgtttctcac
     7801 tcattttttc agaaaccctc cggttgcctt cacacccggt tcacgaccca caaaaggaac
     7861 ggtgaccgga acaggagcca gctggctgac cggtaactgg taaccgtcac gcagactgtg
     7921 gcagaccacc ctctgcacat catccacttc cagttgccat gccggccccg ccattaactg
     7981 cagggcagta cgcagtctca gtggtcccgg ctgatactgc accgccggca gaggctgacg
     8041 gtacagcacg ccgttggccg gtccggtggc gcagagcgtg taaccggact gtttcagggc
     8101 ataacgcatg gcatcggcca ccgtgggttt cagggaggag ggaatgcgaa tgtcgatcac
     8161 ctgagaaagt ggatcacgct gtacagcctg cggctctgtg ctgaccagca ggtaacggtc
     8221 atagcgcacc acctccggtg cctgagaata cacatccggg ctggcaggct gtacattacg
     8281 gtttactgtg accgacggta ccggcatggc cgcatcccgt tgctgtagtt tctgagtgct
     8341 ctgacatccc gccatcagga ggcagactgt cagtccggac aggataaagc gttttttcat
     8401 cgggtattta cctctgcagt gataaatcag aggtcaatgt gcagaagaag acggggctgg
     8461 ctcaatgaac aactcattac ccgctggcga aaaaaaacgc ctgcagacat gcaggcgctt
     8521 tgttgacagg aaagaaagca acataacaaa tatgtcacaa tcctgcggtt aacataaatt
     8581 accgattacc ggagagcatt acgcaaatca tcatccgaga actgcagtat ttgtttgatt
     8641 ttctctgacg atatacccat ctgctgcatg cgacgggccg atgcgtaatt ctcctgaaga
     8701 gccttttctc tctcctcctg gcggcccttc tctatacctt cctggcggcc cttctcgata
     8761 ccttccaggc ggcccttctc aataccttcc tggcggccct tctcgatacc agccagttcc
     8821 agtcgttcag cgatagtcat tagatcatcc tcatgtcttg gcagacgttc tgccatcgcc
     8881 cgtataaatt caggtgaagc tgaatcacat tctttcacca tatagttaaa caatgtcacc
     8941 acctgagtgt gtgtttcctg tatctgaatc aactgtgaca aaaagtcaag atgaaccatc
     9001 agatccactt tcttctgccg gagcttcatc ataagcatca gcgcagccat ctgcttatag
     9061 cgacggatac cctcatcatc aagaagaccg acatcaatca ccctgggtgg cctgccttct
     9121 ccataaagct cccgaccaaa cgccggatca tccagacagt caggccagtt catgctgtat
     9181 ggataaggac tgggtacacc atggtaaaaa agcaccggta tcacaacggg agcacacgca
     9241 tgtgctttca ggtggcgatg tatcaccgcc agaacatagc gcatcattcg cagggccata
     9301 tacttatccg ccttgctctg gtgctctgtc agcgtataaa tgtatccatc cctgccatct
     9361 gtggttttca tactccacag aacatcacag gcaaactggc gaagatccgg atccacaaac
     9421 gtggtcggct ccagttgcag ggtgttcata tcgcaccggt tcagttgttc cgggggcaac
     9481 gcgcctttca ggaaatccct cgcaacagaa ggacattcca tcatcgctct gaacgccgca
     9541 tcatgtggcg tgggggtagt gggtgtgttt tcattcacaa ccgcctcctt gaattaaaag
     9601 tggggcggta agccccgtgg ttaacatcag atgacatgga taatccgtcg ttcaacgcca
     9661 tacccacgcc ccataaaggg gatatcatcg tcaaagtcca tcggcggcat tcccccgctt
     9721 cctgaggaag cctgaccgtt ttttcccgtt gattgttgtg cattttgtgg tggttgtccc
     9781 catcctccct gagcaggagc agatgaaaca tttccaccac cctgctgctg acgcggagac
     9841 cccagcatct gcagggtacc attgacactc acaacaactt ccgtggaata acgatcaaca
     9901 ccattctgct gatcgaacca cttccgggtt cttaactgac cttcaatata cacctgtgaa
     9961 cctttacgaa ggtattcacc ggcaatctca gccagttttc cgtaaataac aacacggtgc
    10021 cattcagtgt gttccttcat ttcaccgctc tgtttatcac gccaggtatc agtggtggcc
    10081 agcgtcagat ttgccactgc acctccgccg ttggggaaat aacggatttc cggatcctgt
    10141 cccagatgac caatcagtat gactttattc actccacgcg atgccataac aactccttat
    10201 cctcacacac attcacgctg agtccagatg ccgttttcca gagcccggac tgcctgctca
    10261 taatttctga aatactcttc ggattcccgg gacacaggcc cttcgtcgca ctgagtacca
    10321 atataataac cacgagcact gctgaggatc tgcagaggta aggtatagcc cgctttggcg
    10381 gccagaattc cgcttgtcgg tttcataaca tgtttcctta aagttaaacg ataagaaaac
    10441 agacctccct gatggttggt ctgttttcat cagggaggtt gagttgaaaa atacgatcag
    10501 aaagaatttt ctgcgtattc ccgcggggta ttgccattct gtggcggtat ggattccgat
    10561 ttttcagtct tataaaccat gtcctggcct attttgatcc actccacctt gatcaggcgg
    10621 gctttcagac tgacgcgttg ctcaccggca tgttcacccc ggttaagggt aaaaatgtca
    10681 gtagaggggt tgctcatggt aaaaccaatc agcaccttgc gatcttcatc caccgctttc
    10741 tggcagcggg caatcaggga agttgcctct ttaccggcaa ccgtcacatc aaaacgcaca
    10801 taggacgggc tgtccgtcgg accactcagt gcattcacaa cacaactgag aaatgtcccg
    10861 ttctgatgat taacctgacg aatgttgctg agatacccca ggcctttcag tgtcagattg
    10921 aagtactcag ttttagcgtt tgcagaagaa gtatttgcag tcataatgat atctccattc
    10981 tcagtatcgg aaagacagag aacatcatca tccctgacgg gttgtgatat tcccgtcagg
    11041 gagccggaac agaactattc cggctgactc agtaacagtt aaag
//
LOCUS       DAZGQL010000137        10930 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.137, whole genome shotgun sequence.
ACCESSION   DAZGQL010000137 DAZGQL010000000
VERSION     DAZGQL010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10930
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.137"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            61..822
                     /gene="adiY"
                     /locus_tag="ACHKIR_004557"
     CDS             61..822
                     /gene="adiY"
                     /locus_tag="ACHKIR_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HFO9992203.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYDSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            959..2296
                     /gene="adiC"
                     /locus_tag="ACHKIR_004558"
     CDS             959..2296
                     /gene="adiC"
                     /locus_tag="ACHKIR_004558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HFO9992204.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            2400..4043
                     /gene="eptA"
                     /locus_tag="ACHKIR_004559"
     CDS             2400..4043
                     /gene="eptA"
                     /locus_tag="ACHKIR_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HFO9992205.1"
                     /translation="MLKRLLKRPSLNLFSWLLLATFYISVCLNIAFFKQVLQVLPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLGSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMITNIIDTTPAESYALMTPRMLLTLGLSGVLAAIIACWIKITPTTSRLRSVLFRGAN
                     ILISVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRSANLPLIR
                     IGEDAHRNPLMQNGKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGVCD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSE"
     gene            4040..4708
                     /gene="basR"
                     /locus_tag="ACHKIR_004560"
     CDS             4040..4708
                     /gene="basR"
                     /locus_tag="ACHKIR_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HFO9992206.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            4709..5809
                     /gene="pmrB"
                     /locus_tag="ACHKIR_004561"
     CDS             4709..5809
                     /gene="pmrB"
                     /locus_tag="ACHKIR_004561"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HFO9992207.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQNLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(5785..5874)
                     /gene="pmrR"
                     /locus_tag="ACHKIR_004562"
     CDS             complement(5785..5874)
                     /gene="pmrR"
                     /locus_tag="ACHKIR_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HFO9992208.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(5986..7488)
                     /gene="proP"
                     /locus_tag="ACHKIR_004563"
     CDS             complement(5986..7488)
                     /gene="proP"
                     /locus_tag="ACHKIR_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HFO9992209.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKHWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVVGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDDIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(7752..8630)
                     /gene="rdcB"
                     /locus_tag="ACHKIR_004564"
     CDS             complement(7752..8630)
                     /gene="rdcB"
                     /locus_tag="ACHKIR_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase regulator RdcB family
                     protein"
                     /protein_id="HFO9992210.1"
                     /translation="MTKTLLDGPGRVLESIHPRFLVDLAQGDDARHPQAHQQQFRERL
                     MQELLARVQLQAWTNGGMLNAPLSLRLTLVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGEQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDAFRLGSPQAVALLLGDLRVKATQHLAESINAAPTTR
                     HYYHQWFASSTIPTGGDHADFLSWLGKWSTADKQPVCWSVTQRWRTVALGMPRLCSAQ
                     RLAGAMVEEIFSVNLV"
     gene            complement(8627..10856)
                     /gene="crfC"
                     /locus_tag="ACHKIR_004565"
                     /pseudo
     CDS             complement(8627..10856)
                     /gene="crfC"
                     /locus_tag="ACHKIR_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418533.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
BASE COUNT         2742 a         2858 c         2674 g         2656 t
ORIGIN      
        1 tcccggtatc gctttgcttt agcgagaact ggtcttttat tcgcactcag gagtacatgt
       61 atgaggattt gcagcgacca accttgtatt gttttattga ctgaaaaaga tgtctggata
      121 agggtgaatg ggaaagaacc tattagcctt aaagctaacc atatggcgtt attaaattgt
      181 gaaaataata ttatcgacgt ctcctctctt aataacactt tggttgctca tattagtcac
      241 gacatcatca aagattacct ccggtttctg aataaagatc tctcgcaaat accagtatgg
      301 caacgtagcg ctacgcccat cctcaccctt ccatgcctga cgccagacgt ctttcgcgtt
      361 gccgcgcaac atagcatgat gcccgctgaa actgagtcag aaaaggaacg aacacgtgca
      421 ttattattca ctgtgctatc ccgttttctc gacagtaaaa aattcctttc actaatgatg
      481 tatatgttac gtaattgtgt aagtgacagc gtttatcaaa ttattgaaag cgatatccac
      541 aaagactgga atcttagtat ggtagccagt tgtttatgtc ttagcccaag tctgttaaag
      601 aaaaagctga aaagcgaaaa caccagttat agccaaataa tcaccacctg ccgcatgcgc
      661 tatgccgtaa atgaattaat gatggacggt aaaaatatct ctcaggtatc acagtcctgc
      721 ggctacgaca gtacgtcgta ctttatttct gtctttaaag acttctacgg tatgacgccg
      781 ctgcattatg ttagtcagca cagagaacgc actgtcgcct gatttttaac cttaacgaag
      841 agctatatta ataacggcat cagcgataac ccggccgata ataattcaac tatcgaatgc
      901 aggcgtatga tacgacgtaa tttattgtca cgaagctcgc cttcgcagga gtttaattat
      961 gtcttcggat gctgatgctc acaaagtggg cttaatcccc gtcaccctga tggtgtcggg
     1021 gaatattatg gggtcaggtg tttttctgtt acctgcaaac ctggcctcca ctggcgggat
     1081 tgccatttat ggatggttgg tgacgattat cggtgcgctg gggctctcga tggtatacgc
     1141 caaaatgtcg ttcctcgacc caagtcctgg tggttcttac gcttacgccc gccgctgctt
     1201 tggcccgttt ctcggttatc aaaccaacgt cctctactgg ctggcctgct ggatcggcaa
     1261 tatcgccatg gtggtcattg gcgtaggata tttaagttac ttcttcccga ttctgaaaga
     1321 cccattggta ttaaccatca cctgcgtcgt ggtgctgtgg atcttcgtcc tgctgaacat
     1381 tgtcggtccg aaaatgatca cccgcgtaca ggccgttgct accgtgctgg cgctgatccc
     1441 catcgtcggg attgccgtat ttggctggtt ctggttccgt ggtgaaacct atatggcggc
     1501 atggaacgtc agcggtctgg gcaccttcgg tgcgattcaa agtaccctta acgttacgct
     1561 gtggtcgttc atcggtgtgg aaagtgcctc cgttgccgca ggtgtggtga aaaacccgaa
     1621 acgcaacgtc cctatcgcca ccattggcgg ggtattgatt gctgccgttt gctatgtact
     1681 ttctaccacc gcgattatgg ggatgatccc taatgccgca ctgcgcgttt ctgcttcgcc
     1741 attcggtgat gccgcacgga tggcgttggg tgacaccgcc ggggccattg tttccttctg
     1801 cgcagctgcg ggttgcttag gttcactggg cggctggacg ttgctggcgg gtcaaacggc
     1861 gaaagccgct gccgatgacg gactgttccc accgattttt gcccgtgtta ataaagcggg
     1921 cacgccagtg gcggggctga ttatcgtcgg tattttgatg accatcttcc agctcagtag
     1981 catttcgcca aacgcgacca aagaattcgg tctggtttct tccgtgtcgg tcatctttac
     2041 actggtgcca tatctttaca cctgtgcggc gttactactg ctcggccacg gtcactttgg
     2101 taaagcacgc ccggcatacc tggcagtgac taccattgcc ttcctctact gcatctgggc
     2161 cgtggtaggg tccggcgcga aagaggttat gtggtcattt gtcactctga tggtaatcac
     2221 cgccatgtat gcgctgaatt acaaccggct gcataaaaac ccgtatccct tagatgcacc
     2281 aataagcaaa gattaattcc ccttaatcaa gcaaacataa aagccaacct taagaactta
     2341 aggttggctt aattttgctt tgcgagcata tgcgcacttt gttcgatgga aacaccgtga
     2401 tgttgaagcg cctactaaaa agaccctctt taaatttatt ctcctggtta ttacttgcta
     2461 ctttttatat ctcagtctgc ctgaatatcg ccttttttaa acaggtgcta caggtgttac
     2521 cgctggattc actgcataac gtgctggttt tcttgtcgat gccggtcgtc gcattcagcg
     2581 tgattaatat tgtcctgaca ctaggctctt tcttatggct taatcgtcca ctcgcctgcc
     2641 tgtttattct ggtcggtgcg gctgcacaat atttcatcat gacctatggc atcgtcatcg
     2701 accgttcaat gatcaccaat atcattgata ctacgccggc ggaaagttat gccctgatga
     2761 cgccacgaat gttattaacg ctggggttaa gtggcgtgtt agccgcgatt attgcctgct
     2821 ggatcaaaat cacccccacc acctcacgcc tgcgcagcgt tcttttccgc ggggccaaca
     2881 tcctgatttc tgtactgctg attctgctgg ttgccgcact gttctacaaa gactatgcct
     2941 cgctgtttcg caacaacaaa gagctggtga aatccttaag cccgtccaac agcattgttg
     3001 ctagctggtc gtggtactct caccagcggt cagccaacct gccactgata cgcattggtg
     3061 aagatgctca ccgcaaccca ttaatgcaaa atgggaaacg taaaaacctg accattttga
     3121 tcgtcggaga aacgtcgcgc gcagagaatt tctcgctgaa tggttatcca cgcgaaacca
     3181 atcctcgtct ggcaaaagat aacgtggtct atttccctaa taccgcctct tgcggcacgg
     3241 caacggcagt ttcagtacca tgcatgttct cggatatgcc gcgtgagcac tacaaagaag
     3301 agctggcaca gcaccaggaa ggcgtgctgg atatcattca gcgagcgggc atcaacgtgc
     3361 tgtggaatga caacgatggc ggctgtaaag gcgtttgcga tcgcgtacct caccagaacg
     3421 tcaccgcgct gaatctgcca ggtcagtgca tcaacggcga atgctatgac gaagtgctgt
     3481 tccacgggct ggaagagtac atcaataacc tgcagggtga tggcgtgatt gtcttacaca
     3541 ccatcggcag ccacggtccg acctattaca accgctatcc gccgcagttc aggaaattta
     3601 ccccaacctg tgacactaac gagatccaga cctgtaccaa agagcaactg gtgaacactt
     3661 acgacaacac gctggtttac gtcgactata ttgttgataa agcgattaat ctgctgaaag
     3721 aacatcagga taaatttacc accagcctgg tttatctttc tgaccacggt gagtcgttag
     3781 gtgaaaatgg catctatctg cacggtctgc cttatgccat cgccccggat agccaaaaac
     3841 aggtgccgat gctgctgtgg ctgtcggagg attatcaaaa acggtatcag gttgaccaga
     3901 actgcctgca aaaacaggcg caaacgcaac actattcaca agataattta ttctccacgc
     3961 tattgggatt aactggcgtt gagacgaagt attaccaggc tgcggatgat attctgcaaa
     4021 cttgcaggag agtgagtgaa tgaaaattct gattgttgaa gacgatacgc tgttattgca
     4081 gggattaatt ctggcggcgc aaaccgaagg ctacgcgtgc gatggcgtga caaccgcgcg
     4141 gatggcggaa caaagccttg aggccggtca ttacagcctg gtggtactgg atttagggtt
     4201 acccgacgaa gatggactgc attttctcgc ccgtatccgg cagaaaaaat acaccctgcc
     4261 ggtactgatc ctcaccgctc gcgatacgct gaccgacaaa atcgccgggc tggatgtcgg
     4321 tgccgatgac tatctggtga agccttttgc gctggaagag ttacacgccc gtatccgcgc
     4381 cctgctacga cgccataata atcagggcga aagtgagctg attgttggca atctgacgct
     4441 gaatatgggt cgccgtcagg tatggatggg cggtgaagag ttgattctga cgcccaaaga
     4501 atatgctctg ctgtcacggt taatgctcaa agcaggcagt ccggtgcatc gggaaattct
     4561 ctacaacgac atctataact gggacaatga accctcgacc aacaccctgg aagtgcatat
     4621 ccacaatctg cgcgacaaag tgggcaaagc ccgtatccgc accgtgcgcg gctttggcta
     4681 tatgctggtc gcaaatgagg aaaactaatt gaatctgatg cgttttctgc gccgaccaat
     4741 atcgctgcgc caacggctga tattgaccat tggggccatt ttgttggtgt ttgagctgat
     4801 cagcgtcttc tggctatggc atgaaagtac cgagcagatt cagctgtttg agcaggcgct
     4861 acgcgacaat cgcaacaacg atcgccacat catgcgtgag atccgcgagg cggtcgccag
     4921 cctgatcgtc cccggcgtct ttatggtcag cctgacgcta tttatctgct atcaggcggt
     4981 acgccgcatc acccggccgc tggcggagct gcaaaaagag ctggaagcgc gcaccgccga
     5041 caacttaacg cctattgcca ttcacagcgc caccctcgaa atcgaagcgg tggtttcggc
     5101 gttgaacgat ctggtcagtc gcctgaccag cacgctggat aacgaaaggt tgtttaccgc
     5161 tgacgtcgcg cacgaactgc gaacgccact ggcgggggtg cgtttgcatc tggaactgct
     5221 ggcgaaaacg catcacattg atgtagcacc gttagtggct cgacttgatc agatgatgga
     5281 gagtgtctcc cagctgctgc aactggcgcg tgccggacag tcattttctt ccggtaatta
     5341 tcaacatgta aaactgctgg aagatgtcat tctcccctcg tatgacgaac tcagtaccat
     5401 gctcgaccag cgacagcaaa acctgctatt gccagagagc gccgccgaca tcaccgttca
     5461 gggtgatgca accctgctgc ggatgttatt gcgaaacctg gtagaaaacg cccatcgtta
     5521 cagcccgcaa ggcagcaaca ttatgattaa gctgcaagaa gatggcggag cggtcatggc
     5581 cgttgaagat gaaggaccgg gtattgatga gagtaaatgc ggggagttga gtaaagcgtt
     5641 tgtacgtatg gacagccgtt atggcgggat tggtctgggg ttaagtattg tcagccgcat
     5701 tacacagttg catcacgggc agtttttcct gcaaaaccgg caagagactt ccggcacgcg
     5761 ggcctgggta cggctgaaga aagatcagaa cgtggcaaac cagatataaa gaaagctgct
     5821 gaccaccagc acgctgaaca cggtagttaa actttcataa acacggtttt tcattgcact
     5881 ctcctccgtt aacctggagg agagtatgcg cgtcagagat taagtgaacc ttaagagttc
     5941 aattggcctg ataagacagc gtcacatcag gccatccgtt tcagcttatt catcaattcg
     6001 cggatgttgc tgcaccaggc gagtacgttt cgcctgcaaa tcggcaatct cgtggtcaat
     6061 atcatcgatt ttctgctcga tattatcgta atgctcgacg agaatttcct tcgcttcctg
     6121 tatatctgac gccgccggtg tcgcaccttt caacggacga tttgccgtct ctttcatggt
     6181 tacgccggtg attaaaccaa ccaccgccac taccatcagg taataggcag gcatcatcag
     6241 attctgcgag ctttcgacca gccaggccgc cagtgttggc gtcagaccgg caaccagcac
     6301 cgaaatatta aatgccgccg ccagcgcgct gtaacggata tgcgtcggaa acatcgctgg
     6361 caaggtagaa gccataacgc ccgtaaagca gttaaggatc accgccagca tcagtaaccc
     6421 ggcaaaaatc aggccgatga cgttactgtt aatcagaata aacgccggga tcgccaacac
     6481 aaacagtgca acactaccaa gtagcacaaa cggacgacgg ccaaaacggt cactcagcaa
     6541 gcccatcacc ggctggacaa acagcatacc gatcataatg gcgataataa tcagcacccc
     6601 gtggtcttcg gaataatgca ggttatgcga caaataactc ggcatatagg tcagcaacat
     6661 gtagtaagtc acgttggtgg caattaccag accaatacat gtcaacaggc tgcgccagtg
     6721 tttggtggca atctctttaa acgagacttt cgggccatcc tgcaaacctt cgcggtcgcc
     6781 ctgctccagt ttatcaacat gctgctggaa cgccggggtc tcttccagcg catggcgcag
     6841 gtaaagcccg ataatcccta acggcagagc aataaagaac ggaatacgcc agccccagtc
     6901 gaggaagttc gcttcgccga caatggtcga aattaacacc accacgcccg cacccagcac
     6961 aaacccggca atagaaccga agtccagcca gctgcccata aagccacgtt tacggtcagg
     7021 ggagtattcc gcaacaaata tcgacgcccc ggtatattca ccgccgaccg agaaaccttg
     7081 tgccatctta cagatcaaca gcagaatcgg tgcccaaata ccaatcgtgt cgtaggacgg
     7141 tattaagcca atacagaacg tactgatcga cataatcaca atagtgatag cgaggatctt
     7201 ctggcgacca tatttatcgc ccaacatacc aaagaagagt ccaccaagcg gtcgaatcag
     7261 aaagggaacg gagaaagtgg caagtgcagc aaccatctgc acgctggggt cagcccccgg
     7321 gaaaaaaact ttacctaatg cgtaagcaac aaaaccataa acaccaaaat caaaccattc
     7381 cattgcatta cccagtgatg ctgcggtaat ggctttacgc agtttaccgt catcaataat
     7441 ggtgacatca cgaagggtaa ttggttttac ttttttcctt ttcagcatag ctttcctcgc
     7501 agagttggcc catttccgca aacgccagaa aaatcagcgc gacggccgca aaaggggagc
     7561 gggaattgca ggatgcaatc tctgtaacaa gcgtagcggg tttgcttaca ctctccggtt
     7621 aaggaatgta caatcaaacc agttgacgcc tgaattgggc agttaatgac ctttttaccc
     7681 taccagcgtt taaatttgtg atcaacttca cacatggatt tcgtttagga ctcattgatg
     7741 taactgatta tttacaccaa atttacagag aagatttcct cgaccattgc ccccgccaga
     7801 cgctgcgctg aacagagtcg tggcatcccc agtgcgacag ttcgccagcg ttgggtcact
     7861 gaccagcaaa cgggttgttt atctgccgtg gaccactttc ccagccaact taaaaaatca
     7921 gcatgatccc cgcccgtcgg aatggtcgaa gaggcaaacc actgatggta atagtgacgc
     7981 gtggtcgggg ccgcattgat actctctgcc agatgttgtg tcgctttcac gcgcaaatca
     8041 cccagcaaca acgccaccgc ttgcgggctt cccagacgaa aggcatcgcc aaatgccccc
     8101 caacgcaact cctccagaac gataaaacag cgcccaggca acgaccaggc gttgtacgcc
     8161 ccagcacgcc agcgggtaaa aatttgctcg ctctgttcac ccgcctgcac cagtaggcct
     8221 cgttgcgtta acgccttttc cttccagcga caacgggctg aaaaccactc atacaaggcg
     8281 gcaatttgct gggggagctc agggaaagca gagtgctggc ggtgatccat tttttgcagc
     8341 agcgccagat gttgcgcgat ctgcgtcagc gccagatgac cgggatccag catcgacgcc
     8401 agtttttcca ccaatgtcag acgcaggcta agcggcgcat ttaacatccc gccgttcgtc
     8461 catgcctgaa gctgcacacg cgcaagaagt tcctgcatca gtcgttcacg aaactgctgc
     8521 tgatgggctt gtggatggcg ggcatcatcg ccctgcgcca gatccactaa aaagcgggga
     8581 tgaatcgact ccagcacgcg accggggccg tccagtaacg tcttggtcaa tatcgttctg
     8641 ccgtgaaaag tgtttgaata tcatcgcgta acagctgagc gtcggtgtaa atccagccat
     8701 gagtcatcac agtctgctgc aattgctgct gcatcagcct gaccaccgat tcattttgtt
     8761 gacgcagagc cagactttcg cgtaaacgcg tctgtaattc cgtcaaacat gaagcgaact
     8821 cagcgaaaaa agtattcatg cctgccgtaa cagattcatc gacctgctct gccagaactt
     8881 tagccatttg ttggcaataa agatcgactt ctgcgcttaa tgctcgttgc aacgcactgt
     8941 aatcaaccgt ctctatcggg gatttctcat ttccccgtcc ccagtcgggc tgattcaacc
     9001 agcgcgaaaa agtctcacgc acaacgccta aacgcgtgct ctgctcgtcc attgcgtcct
     9061 ggcgcgaaat gacttcactg aacagctggc gagtgttgaa gtggggaact acgccgtgaa
     9121 aaacaggaaa atgaaaccca ggacgaaacc ctgactcgct caattccatt ttgatttgtt
     9181 gctcaatggg gcgaataaca tcggttaaca cccgggcaaa gggtggattc cagctcggca
     9241 aaacgcagcg taaagtcgcg actaatggtg ttctgcgcca tctgtaacag tgtctcacag
     9301 caggtacgaa tctcgcttaa tgaccctgaa tcatcctgaa acaaggcggc taactgcgca
     9361 ttcagcgcat cttgctgttg acgcagaaag tggttggcgg aggtcagggc cagctcgatt
     9421 tcatgtttaa tctcgccgct cacctgcgcc tgattgagtt gcaatagctg caaactttct
     9481 tcgatctgat ggatattttg ccgcaattgt tcacaagcga cgtttaaccc gtgcgcacga
     9541 aaatccaggt attcccgcgc ctgctgcgcg taattcaaca gtttatgcgc ggcagatcgc
     9601 agagcataca acgaggcgtt agcgtaagcg gcatgaagca acgcctgaat tggctgggcg
     9661 aacagcgaat cttcccacaa ctgatcggca gcatgacgaa tatgttcgag gtccgccaga
     9721 tcggcatgac gccagcgcct gccgagcgcg gcatgggcaa aatcttccac ccagcgttgt
     9781 tgctctggcg gtggtaactt accgttgttg gctaactcat ggcgcacccg attcgccagg
     9841 tagccccaca tcgacgacac cggaaatatc tgctgtggcg taatacaacc tttcatcagc
     9901 gtcccggaaa tcagtgcccg cacctggtcg gcgtcgtcac tgttacgatc ctgttgatcg
     9961 aacttattga ccagcacata caacggcacc gattgcccca ccgccaaaat tgcctcacgg
    10021 acctcttcat cggagatcga tttcagttgc gtataatcca gcaccgccag taccgccgag
    10081 gcgcgtgcca gctgctggtt aagcattttt tgcagatgcg gttgcccggc ttcatttggc
    10141 ccgggggtat ccagtaacgt caactgaccg ggataactct ccagccccgc cagatggaca
    10201 aactccactt caatcacggg aatatgctca atggcggcgt aagcagaaaa aggaaaatcg
    10261 acgtccagcg ccttcgccag tcgcaccaaa tcattcaaac ttttcagaca atgaaaaata
    10321 ggttgagcac ccagataata tttttcgaaa gcgatgccat tttcaatccg ctgcataagc
    10381 gcacgcatat ctttatctat ttccagcaca tcggtcagat gcttaatatc gcaatcacgc
    10441 aggcgctgtt gtaattgttg aattaaatgg tcgattggcg cgacatgtga aaaatgcagt
    10501 accggttctt tttgcccggg cgtatggcga ataagcgttg gcagcgcagt cattgggcga
    10561 ttacgattag gcagaacctc cgtaccaaca atggcattaa tggtggttga tttccctgct
    10621 ttcatggtac cgacaatcgc cagcaccatt tccagccggg aaattttacg caactcatta
    10681 ttcagcatcg cgtgacgttc ggcgatatta ggctgactcc agggtaaagc cagttgtggc
    10741 gtatcgtctc cgggtacaga gagaggtatt ttttccagta actgcaactg ttggcgagaa
    10801 agctgtaaca ggcgttcagc ctcctgactt aactcataca gggtctgtgt atacatagaa
    10861 tattcttcct taaagcaaat tttattactt ttatttagcc agattgtttt tgagttctgt
    10921 tttcggcttt
//
LOCUS       DAZGQL010000138        10814 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.138, whole genome shotgun sequence.
ACCESSION   DAZGQL010000138 DAZGQL010000000
VERSION     DAZGQL010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10814
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.138"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            197..1363
                     /gene="nhaA"
                     /locus_tag="ACHKIR_004566"
     CDS             197..1363
                     /gene="nhaA"
                     /locus_tag="ACHKIR_004566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HFO9992211.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNHADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            1423..2328
                     /gene="nhaR"
                     /locus_tag="ACHKIR_004567"
     CDS             1423..2328
                     /gene="nhaR"
                     /locus_tag="ACHKIR_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HFO9992212.1"
                     /translation="MSMSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRAL
                     EERLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVA
                     DALSKRLVSSVLDAAVVEDEQIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQE
                     GLFSMKIGECGVSFWCTNPLPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNV
                     EILGEFDDAALMKAFGATHNAIFVAPSLYANDFYNDDSVVEIGRVENVMEEYHAIFAE
                     RMIQHPAVQRICNTDYSALFTPASK"
     gene            complement(2424..2687)
                     /gene="rpsT"
                     /locus_tag="ACHKIR_004568"
     CDS             complement(2424..2687)
                     /gene="rpsT"
                     /locus_tag="ACHKIR_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFO9992213.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            2790..3008
                     /locus_tag="ACHKIR_004569"
     CDS             2790..3008
                     /locus_tag="ACHKIR_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HFO9992214.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSARKIAAGGQFLPMICAIAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            3016..3957
                     /gene="ribF"
                     /locus_tag="ACHKIR_004570"
     CDS             3016..3957
                     /gene="ribF"
                     /locus_tag="ACHKIR_004570"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFO9992215.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            4000..6816
                     /gene="ileS"
                     /locus_tag="ACHKIR_004571"
     CDS             4000..6816
                     /gene="ileS"
                     /locus_tag="ACHKIR_004571"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFO9992216.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIIKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSLSIDVAFQAVD
                     QDALKTKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVSDYTILGTVKGAELELLRFTHPFMDFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVIALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCANAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            6816..7310
                     /gene="lspA"
                     /locus_tag="ACHKIR_004572"
     CDS             6816..7310
                     /gene="lspA"
                     /locus_tag="ACHKIR_004572"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFO9992217.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            7418..7867
                     /gene="fkpB"
                     /locus_tag="ACHKIR_004573"
     CDS             7418..7867
                     /gene="fkpB"
                     /locus_tag="ACHKIR_004573"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFO9992218.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            7869..8819
                     /gene="ispH"
                     /locus_tag="ACHKIR_004574"
                     /gene_synonym="lytB"
     CDS             7869..8819
                     /gene="ispH"
                     /locus_tag="ACHKIR_004574"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HFO9992219.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKGVNCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            8885..9799
                     /gene="rihC"
                     /locus_tag="ACHKIR_004575"
     CDS             8885..9799
                     /gene="rihC"
                     /locus_tag="ACHKIR_004575"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HFO9992220.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFTPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRQPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALVP"
     gene            9966..10787
                     /gene="dapB"
                     /locus_tag="ACHKIR_004576"
     CDS             9966..10787
                     /gene="dapB"
                     /locus_tag="ACHKIR_004576"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFO9992221.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNNL"
BASE COUNT         2464 a         2618 c         3028 g         2704 t
ORIGIN      
        1 taagctatga ttatcagggc ttagggtcac acgtcagcgc ttacagccgt caaaaactca
       61 tctcatcact gatgacgcaa atccttcaat gcctcgtaaa aagcgaatta ttcctacact
      121 ataatctgat tttaacgatg attcgtgcgg ggtaagatag taaaaacgat ctattcacct
      181 gaaagagaaa taaaaagtga aacatctgca tcgattcttt agcagtgatg cctcgggagg
      241 cattattctt atcattgccg ctatcctggc gatgattatg gccaacagcg gcgcaaccag
      301 tggatggtat cacgactttc tggagacgcc ggttcagctc cgggttggtt cactcgaaat
      361 caacaaaaac atgctgttat ggataaatga cgcgctgatg gcggtatttt tcctgttagt
      421 cggtctggaa gttaaacgtg aactgatgca aggatcgcta gccagcttac gccaggctgc
      481 atttccagtt atcgccgcta ttggtgggat gattgtgccg gcattactct atctggcctt
      541 taaccatgcc gatccgatta cccgcgaagg gtgggcgatc ccggcggcta ctgacattgc
      601 ttttgcactt ggtgtgctgg cgctgttggg aagtcgtgtt ccgttagccc tgaagatctt
      661 tttgatggct ctggctatta tcgacgatct tggggccatc attatcatcg cattgttcta
      721 cactaatgac ttatcgatgg cctctcttgg cgtcgcggct gtagcaattg cggtactcgc
      781 ggtattgaat ctgtgtggtg tacgccgcac gggcgtctat attctggttg gcgtggtgtt
      841 gtggactgcg gtgttgaaat cgggggttca cgcaaccctg gcgggggtaa ttgtcggctt
      901 ctttattcct ttgaaagaga agcatgggcg ttctccagcg aagcgactgg agcatgtgtt
      961 gcacccgtgg gtggcgtatc tgattttacc gctgtttgca tttgctaatg ctggcgtttc
     1021 actgcaaggc gtcacgctgg atggcttgac ctccattctg ccattgggga tcatcgctgg
     1081 cttgctgatt ggcaaaccgc tggggattag tctgttctgc tggttggcgc tgcgtttgaa
     1141 actggcgcat ctgcctgagg gaacgactta tcagcaaatt atggcggtgg ggatcctgtg
     1201 cggtatcggt tttactatgt ctatctttat tgccagcctg gcctttggta gcgtagatcc
     1261 agaactgatt aactgggcga aactcggtat cctggtcggt tctatctctt cggcggtaat
     1321 tggatacagc tggttacgcg ttcgtttgcg tccatcagtt tgacaggacg gtttaccggg
     1381 gagccataaa cggctccctt ttcattgtta tcagggagag aaatgagcat gtctcatatc
     1441 aattacaacc acttgtatta cttctggcat gtctataaag aaggttccgt ggttggcgca
     1501 gcggaggcgc tttatttaac accacaaacc attaccggac agattcgggc gctggaagag
     1561 cgcctgcaag gcaaattatt taagcgcaag ggacgtggcc tcgagcccag cgagctggga
     1621 gagctggtct atcgctatgc cgataaaatg ttcaccttaa gccaggaaat gctggatatc
     1681 gtcaactatc gcaaagagtc caacttattg tttgatgttg gtgtggcaga tgcactttcc
     1741 aaacgtctgg tcagcagtgt tctggatgcc gcagttgtgg aagacgagca gatccatcta
     1801 cgctgtttcg aatcgacgca cgagatgctt ttagagcagt tgagtcagca taaactggat
     1861 atgatcatct ctgactgtcc gatcgattcc actcagcagg aagggctgtt ttccatgaaa
     1921 attggcgaat gtggtgtcag tttctggtgc actaacccac taccagaaaa gccgtttcct
     1981 gcctgtcttg aagagcgtcg tttacttatt ccggggcgtc gctcaatgtt ggggcgtaag
     2041 ctattaaact ggtttaactc ccagggcttg aacgtcgaaa ttttgggtga gtttgatgat
     2101 gctgcgttga tgaaagcctt tggggcgacg cataacgcta ttttcgttgc accttcgctt
     2161 tacgctaatg atttctataa cgatgactcg gttgtggaga taggccgtgt tgagaacgtg
     2221 atggaagagt accacgcgat ttttgccgaa aggatgattc agcaccctgc agtacagcgt
     2281 atctgcaata cagactattc tgcgctgttt actccagctt caaaataaac atttgtactt
     2341 tgcaggccga attttggcct gcagatggat ataaaaaaac ccgccagagc gggttttttt
     2401 acaaagctga caacaacagg caattaagcc agtttgttga tctgtgcagt caggttagcc
     2461 ttatgacgtg cagctttgtt tttgtggatc agacctttag cagcctgacg gtccacgatt
     2521 ggttgcattt cgttaaatgc tttctgtgca gcagctttgt cgccagcttc gatagctgcg
     2581 tatactttct tgatgaaagt acgcatcata gagcgacggc ttgcgttgtg cttacgagcc
     2641 ttttcagact gaatggcgcg cttcttagct gatttgatat tagccaaggt ccaactccca
     2701 aatgtgttct atatggacaa ttcaaaggcc gaggaatatg cccttttagc cttcttttgt
     2761 caatggattt gtgcaaataa gcgccgttaa tgtgccggca ctcgttacgt agtgatggcg
     2821 caggattcta ccagcttgcg gggtgtgaat acagcttttc cgcgagaaaa attgcagcag
     2881 gcggtcagtt tcttcccatg atttgcgcca tagcaatgaa aagccacttc tttctgattt
     2941 cggtactcaa tcgccggtta accttgaccg ctgtacaagg tatactcgga cgattttcac
     3001 tgttttgagc cagacatgaa gctgatacgc ggcatacata atctcagcca ggccccgcaa
     3061 gaagggtgtg tgctgactat tggtaatttc gacggcgtgc atcgcggtca tcgcgcgctg
     3121 ttacagggct tgcaggaaga agggcgcaag cgcaacttac cggtgatggt gatgcttttt
     3181 gaacctcaac cactggaact gtttgctact gataaagccc cggcacggct cacccggctg
     3241 cgggaaaaac tgcgttatct tgcagagtgt ggtgttgatt acgtgctgtg cgtgcgtttt
     3301 gacaggcgtt ttgcggcgtt aaccgcgcaa aacttcatca gtgatcttct ggtgaagcac
     3361 ttgcgggtaa aatttcttgc cgtaggtgac gatttccgct ttggcgctgg tcgtgaaggc
     3421 gatttcttgt tattacagaa agcgggcatg gaatacggct tcgatatcac cagcacgcaa
     3481 actttttgcg aaggtggcgt gcgtatcagc agcaccgccg tgcgtcaggc gcttgcggat
     3541 gacaatctgg ctctggcaga aagtttactg gggcacccgt ttgctatctc cgggcgtgta
     3601 gtccacggtg atgaattagg gcgcactata ggtttcccga cggcgaatgt accgctacgc
     3661 cgtcaggttt ccccggtgaa aggggtttat gcggtagaag tgttgggcct tggcgaaaag
     3721 ccgttacccg gcgttgcaaa catcggaaca cgcccaacgg ttgccggtat tcgccagcaa
     3781 ctggaagtgc atttgttaga tgttgcaatg gacctttatg gtcgccatat acaagtagtg
     3841 ctgcgtaaaa aaatacgcaa tgagcagcga tttgcatcgc tggacgaact gaaagcgcag
     3901 attgcgcgtg atgaattaac cgcccgcgaa ttttttgggc taacaaaacc ggcttaagcc
     3961 tgttatgtaa tcaaaccgaa atacggaacc gagaatctga tgagtgacta taaatcaacc
     4021 ctgaatttgc cggaaacagg gttcccgatg cgtggcgatc tcgccaagcg cgaaccggga
     4081 atgctggcgc gttggactga tgatgatctg tacggcatca ttcgtgcagc taaaaaaggc
     4141 aaaaaaacct tcattctgca tgatggccct ccttatgcga atggcagtat tcatattggt
     4201 cactcggtta acaagattct gaaagacatt atcattaagt ccaaagggct ttctggatat
     4261 gactcgccgt atgtgcctgg ctgggactgt catggtctgc caatcgagct gaaagtagag
     4321 caagaatacg gtaagccggg ggagaaattc accgccgctg agttccgcgc caagtgccgc
     4381 gaatacgctg cgacccaggt tgacggtcag cgcaaagact ttatccgtct gggcgtgctg
     4441 ggcgactggt cgcacccgta cctgaccatg gacttcaaaa ctgaagccaa catcatccgc
     4501 gcgctgggca aaatcatcgg caacggtcac ctgcacaaag gcgcgaagcc ggtgcactgg
     4561 tgcgttgact gccgttctgc actggcagaa gcggaagttg agtattacga caaaacttct
     4621 ctgtccatcg acgtcgcttt ccaggcggtc gatcaggatg cgcttaaaac gaaatttggc
     4681 gtaagcaatg ttaacggccc aatttcgctg gttatatgga ccaccacgcc gtggacgctg
     4741 cctgctaacc gcgcaatctc catcgcacct gattttgatt atgcgctggt gcaaatcgac
     4801 ggtcaggccg tgatcctcgc gaaagatctg gttgaaagcg taatgcagcg tatcggcgtt
     4861 agcgattaca ccattcttgg cacggtgaaa ggtgccgagc tggaactgtt gcgctttacc
     4921 catccgttta tggacttcga tgttccggca attctcggcg accacgttac gctggatgcc
     4981 ggtaccggtg ccgttcatac cgcgccaggc cacggtccgg acgactatgt gatcggtcaa
     5041 aaatatggtc tggaaaccgc taacccggtt ggcccggacg gcacttatct gccgggtact
     5101 tacccgactc tggatggcgt taacgtcttc aaagcgaacg atattgtcat tgcgttgttg
     5161 caggaaaaag gcgcactgtt gcacgttgag aaaatgcaac acagctatcc gtgctgctgg
     5221 cgtcataaaa cgccgatcat cttccgcgcg acgccgcagt ggttcgtcag catggatcag
     5281 aaaggtctgc gtgcgcagtc actgaaagag atcaaaggcg tgcagtggat ccctgactgg
     5341 ggccaggcgc gtatcgagtc gatggttgct aaccgtcctg actggtgtat ctctcgtcag
     5401 cgtacctggg gcgtgccgat gtcactgttc gtgcacaaag acacagaaga actgcatccg
     5461 cgtactctcg aactgatgga agaagtggca aaacgcgttg aagttgacgg cattcaggcg
     5521 tggtgggatc tcgatgcgaa agagatcctc ggcgacgaag ctgaccagta tgtgaaagta
     5581 ccggatacgc tggatgtatg gtttgactcc ggatctaccc actcttccgt tgttgatgtg
     5641 cgtccggaat ttgccggtca cgcagcggac atgtatctgg aaggttctga ccaacaccgt
     5701 ggctggttca tgtcttccct gatgatctct accgcgatga agggcaaagc gccatatcgt
     5761 caggtactga ctcacggctt taccgtggat ggtcagggtc gcaagatgtc taaatccatc
     5821 ggtaacaccg tttcgccgca ggatgtgatg aacaaactgg gtgcggatat tctgcgtctg
     5881 tgggtggcat caaccgacta cactggcgaa atggccgttt ctgacgagat cctgaaacgt
     5941 gctgccgaca gctatcgtcg tatccgtaac accgcgcgct tcctgctggc aaacctgaac
     6001 ggttttgatc cggcaaaaga tatggtgaaa ccggaagaga tggtggtact ggatcgctgg
     6061 gccgtaggtt gtgcgaacgc ggcacaggaa gacatcctca aagcgtacga agcatacgat
     6121 ttccacgaag tggtacagcg tctgatgcgc ttctgctccg ttgagatggg gtccttctac
     6181 ctcgacatca tcaaagaccg tcagtatacc gccaaagcgg acagcgtggc gcgtcgtagc
     6241 tgccagactg cgctgtatca catcgcagaa gcgctggttc gctggatggc accaatcctc
     6301 tccttcaccg ctgatgaagt gtggggttac ctgccgggcg aacgtgaaaa atacgtcttc
     6361 accggtgagt ggtacgaagg cctgtttggt ctggcagaca gtgaagcaat gaacgatgcg
     6421 ttctgggacg agctgttgaa agtgcgtggc gaagtgaaca aagtcattga gcaagcgcgt
     6481 gccgataaga aagtgggcgg ctcgctggaa gcggcagtaa ccttgtatgc agaaccggaa
     6541 ctggcggcga aactgaccgc gctgggcgat gaattacgat ttgtcctgtt gacctccggc
     6601 gctaccgttg cagactataa cgacgcacct gctgacgccc agcagagcga agtcctcaaa
     6661 gggctgaaag tcgcgttgag taaagccgaa ggtgagaagt gcccacgctg ctggcactac
     6721 acccaggatg tcggcaaggt ggcggaacac gcagaaatct gcggccgttg tgtcagcaac
     6781 gtcgccggtg acggtgaaaa acgtaagttt gcctgatgag tcaatcgatc tgttcaacag
     6841 ggctacgctg gctgtggctg gtggtagtcg tgctgattat cgatctgggc agcaaatacc
     6901 tgatcctcca gaactttgct ctgggggata cggtcccgct gttcccgtcg cttaatctgc
     6961 attatgcgcg taactatggc gcggcgttta gtttccttgc cgatagtggc ggctggcagc
     7021 gttggttttt tgccggtatt gcgattggta ttagcgtgat cctggcagtg atgatgtatc
     7081 gctcgaaggc cacgcagaag ctaaacaata tcgcttacgc gctgattatt ggcggcgcgc
     7141 tgggcaacct gttcgaccgc ctgtggcacg gcttcgttgt cgatatgatc gacttctatg
     7201 tcggcgactg gcacttcgcc accttcaacc ttgccgatac tgccatttgt gtcggtgcgg
     7261 cactgattgt gctggaaggt tttttacctt ctaaagcgaa aaaacaataa taaaccctgc
     7321 cggatgcgat gctgacgcat cttatccggc ctacagattg ctgcgaaatc gtaggccgca
     7381 tccggcaaaa atcctcaaaa tataagagca aacctgcatg tctgaatctg tacagagcaa
     7441 tagcgccgtc ctggtgcact tcacgctgaa actcgatgat ggcaccaccg ctgagtctac
     7501 ccgcaacaac ggtaaaccgg cgctgttccg cctgggtgat gcttctcttt ctgaagggct
     7561 ggagcaacac ctgctggggc tgaaagtggg cgataaaacc accttctcgc tggagcctga
     7621 tgcggcgttt ggcgtgccgt caccggacct gattcagtac ttctcccgcc gtgaatttat
     7681 ggatgcaggc gagccagaaa ttggcgcaat catgcttttt accgcaatgg atggcagtga
     7741 gatgcctggc gtgatccgcg aaattaacgg cgactccatt accgttgatt tcaaccatcc
     7801 gctggccggg cagaccgttc attttgatat tgaagtgctg gaaatcgatc cggcactgga
     7861 ggcgtaacat gcagatcctg ttggccaacc cgcgtggttt ttgtgccggg gtagaccgcg
     7921 ctatcagcat tgttgaaaac gcgctggcca tttacggcgc accgatatat gtccgtcacg
     7981 aagtggtgca taaccgctac gtggtcgata gcttgcgcga gcgtggggct atctttattg
     8041 agcagattag cgaagtaccg gacggcgcga tcctgatttt ctccgcacac ggtgtttctc
     8101 aggcagtacg taacgaagcg aaaagccgcg atttgacggt gtttgatgcc acctgtccgc
     8161 tggtgaccaa agtgcatatg gaagtcgccc gcgccagccg tcgtggtgaa gaatctattc
     8221 tcatcggtca cgccgggcac ccggaagtgg aagggacgat gggccagtac agcaacccgg
     8281 aagggggaat gtatctggtc gaatcgccag acgatgtgtg gaaactgacg gtcaaaaacg
     8341 aagagaagct ctcctttatg acccaaacca cgctgtcggt ggatgacacg tctgatgtga
     8401 tcgacgcgct gcgtaaacgc ttcccgaaaa ttgtcggtcc gcgcaaagat gacatctgct
     8461 acgccacgac taaccgtcag gaggcggtac gcgccctggc agagcaggcg gaagtagtac
     8521 tggtggttgg ttcgaaaaac tcctccaact ccaaccgtct ggcggaactg gcccagcgta
     8581 tgggcaaacg cgcatttttg attgacgatg cgaaagatat ccaggaagag tgggtaaaag
     8641 gcgtgaactg cgttggcgtg actgcgggcg catcggctcc ggatattctg gtgcagaatg
     8701 tggtggcacg tttgcaacag ctgggcggtg gtgaagccat tccgctggaa ggtcgtgaag
     8761 aaaacattgt tttcgaagtg ccgaaagagc tgcgtgtcga tattcgtgaa gtcgattaag
     8821 tcattggcag cctaagttat gcgaaaatgc cggtcttgtt accggcattt tttatggaga
     8881 aaacatgcga ttacctatct tcctcgatac agaccccggc attgacgatg ccgttgccat
     8941 tgccgccgcg atttttaccc ccgaactcga tctgcaactg atgaccactg tcgcgggtaa
     9001 tgtttcggta gagaaaacga cccgcaacgc cctgcaactg ctgcatttct ggaatgcgga
     9061 gattccgctt gcccagggcg ctgccgtacc actggttcgt gcgccgcgcg atgcggcatc
     9121 tgtacacggt gaatcgggaa tggctggcta tgactttgtt gaacacaacc gtcagccact
     9181 gggtataccg gcgtttctgg caattcgtga tgccctgatg cgtgcgccag agcccgttac
     9241 cctggtggcc atcggcccgt taaccaatat tgcgctgtta ctttcacaat gcccggaatg
     9301 taagccgtat attcgccgtc tggtgatcat gggcggttct gctggtcgcg gcaactgcac
     9361 accaaacgcc gagtttaata tcgctgccga tccagaagcg gcagcctgtg tcttccgtag
     9421 cggcatcgaa atcgtcatgt gcggtctgga tgtcaccaat caggcaatat taacccctga
     9481 ctatctcgct accttgccgg agttgaatcg caccggaaaa atgctccacg ccctgtttag
     9541 tcactaccgt agcggcagta tgcaaagtgg actgcgaatg cacgatctct gcgccatcgc
     9601 ctggctggtg cgcccggacc tgttcactct caagccgtgt tttgtggcag tggaaacgca
     9661 gggcgaattt acctctggca cgacggtggt tgatatcgac ggttgcctgg gcaaaccagc
     9721 caatgtacag gtggcattgg atctgaatgt aaaaggcttc cagcagtggg tagctgaggt
     9781 gctggctctg gtgccgtaac ctgtcacatg ttatcggcat gcagtcattc atcgactcat
     9841 gcctttcact gatatccctc cctgtttatc attaatttct aattatcagc gtttttggct
     9901 ggcggcgtag cgatgcgctg gttactctga aaacggtcta tgcaaattaa caaaagagaa
     9961 tagctatgca tgatgcaaac atccgcgttg ccatcgcggg agccgggggc cgtatgggcc
    10021 gccagttgat tcaggcggcg ctggcattag aaggcgtgca gctgggcgcg gcgctggagc
    10081 gcgaaggatc ttctttactg ggcagcgatg cgggcgaact ggccggagcc gggaaaacag
    10141 gcgttactgt gcaaagtagt cttgatgcga taaaagatga ttttgatgtg tttatcgatt
    10201 ttacccgtcc ggaaggtacg ctgaaccatc tcgctttttg tcgccagcat ggcaaaggga
    10261 tggtgatcgg cactacgggg tttgacgaag ccggtaaaca ggcgattcgt gacgccgccg
    10321 ccgatattgc gattgtcttt gccgccaatt ttagcgttgg cgttaacgtc atgcttaaac
    10381 tgctggagaa agcagccaaa gtgatgggtg actacaccga tatcgaaatt attgaagcac
    10441 atcatagaca taaagttgat gcgccgtcag gcaccgcact ggcaatggga gaggcgatcg
    10501 cccacgctct ggataaagat ctgaaagatt gcgcggtcta cagtcgtgaa ggccataccg
    10561 gtgaacgtgt gcctggcacc attggttttg ccaccgtgcg tgcaggtgac atcgttggtg
    10621 aacataccgc gatgtttgcc gatattggcg agcgtctgga gatcacccat aaggcgtcca
    10681 gccgcatgac atttgctaac ggtgcggtaa gatcggcttt gtggttgagt ggtaaggaaa
    10741 gtggtctttt tgatatgcga gatgtgcttg atctcaataa tctgtaacca caaaatattt
    10801 atagtgatgc aaaa
//
LOCUS       DAZGQL010000139        10794 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.139, whole genome shotgun sequence.
ACCESSION   DAZGQL010000139 DAZGQL010000000
VERSION     DAZGQL010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10794
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.139"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            153..1295
                     /gene="yghO"
                     /locus_tag="ACHKIR_004577"
     CDS             153..1295
                     /gene="yghO"
                     /locus_tag="ACHKIR_004577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YghO"
                     /protein_id="HFO9992222.1"
                     /translation="MECDLLMIKIEKVINKNDLKAFIAFPSSLYPDDPNWIPPLFIER
                     SEHLSAKNPGTDHIIWQAWVAKKAGQIVGRITAQIDTLHRERYGEDTGHYGMIDAIDD
                     PQVFAALFGAAEAWLKSQGANKISGPFSLNINQESGLLIEGFDTPPCAMMPHGKPWYA
                     AHIEQLGYHKGIDLLAWWMQRTDLTFSPALKKLMDQVRKKVTIRCINRQRFAEEMQIL
                     REIFNSGWQHNWGFVPFTEHEFATMGDQLKYLVPDDMIYIAEIDSAPCAFIVGLPNIN
                     EAIADLNGSLFPFGWAKLLWRLKVSGVRTARVPLMGVRDEYQFSRIGPVIALLLIEAL
                     RDPFARRKIDALEMSWILETNTGMNNMLERIGAEPYKRYRLYEKQI"
     gene            complement(1302..2066)
                     /gene="glcC"
                     /locus_tag="ACHKIR_004578"
     CDS             complement(1302..2066)
                     /gene="glcC"
                     /locus_tag="ACHKIR_004578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001305094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HFO9992223.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSRVARLNRVQDASPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENNKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMKQG"
     gene            2317..3816
                     /gene="glcD"
                     /locus_tag="ACHKIR_004579"
     CDS             2317..3816
                     /gene="glcD"
                     /locus_tag="ACHKIR_004579"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009339 - glycolate oxidase complex
                     [Evidence IEA];
                     GO_function: GO:0003973 - (S)-2-hydroxy-acid oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
                     /protein_id="HFO9992224.1"
                     /translation="MSILYEERLDGALPDVDRTSVLMALREHVPGLEILHTDEEIIPY
                     ECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLRVPVVTRGAGTGLSGGALPLEKGV
                     LLVMARFKEILDINPVGRRARVQPGVRNLAISQAVAPHNLYYAPDPSSQIACSIGGNV
                     AENAGGVHCLKYGLTVHNLLKIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLG
                     VTTEVTVKLLPKPPVARVLLASFDSVEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAE
                     DFIHAGYPVDAEAILLCELDGVESDVQEDCERVNDILLKAGATDVRLAQDEAERVRFW
                     AGRKNAFPAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRVANVFHAGDGNM
                     HPLILFDANEPGEFARAEELGGKILELCVEVGGSISGEHGIGREKINQMCAQFNSDEI
                     TTFHAVKAAFDPDGLLNPGKNIPTLHRCAEFGAMHVHHGHLPFPELERF"
     gene            3816..4868
                     /gene="glcE"
                     /locus_tag="ACHKIR_004580"
     CDS             3816..4868
                     /gene="glcE"
                     /locus_tag="ACHKIR_004580"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HFO9992225.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGTGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRATLSLRREISLQEAMNEIAQWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTADDNQIHRIARNAGGHATRFSAGDGGFAPLPAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            4879..6102
                     /gene="glcF"
                     /locus_tag="ACHKIR_004581"
     CDS             4879..6102
                     /gene="glcF"
                     /locus_tag="ACHKIR_004581"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HFO9992226.1"
                     /translation="MQTRLTEEMRQNARALEADSILRACVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLIKQVLEGNEVTLKTQEHLDRCLTCRNCETTCPSGVRYHNLLDIGRDIV
                     EQKVKRPLPERMLREGLRQVVPRPAVFRALTQVGLVLRPFLPEQVRAKLPAETVKAKP
                     RPPLRHKRRVLMLEGCAQPTLSPNTNAATARVLDRLGISVTPANEAGCCGAVDYHLNA
                     QEKGLARARNNIDAWWPAIEAGVEAILQTASGCGAFVKEYGQMLKNDALYADKARLVS
                     ELAVDLVELLREEPLEKLAIRGDKKLAFHCPCTLQHAQKLNGEVEKVLLRLGFTLTDV
                     PDSHLCCGSAGTYALTHPDLARQLRDNKMNALESGKPEMIVTANIGCQTHLASADRTS
                     VRHWIEIVEQALEKE"
     gene            6107..6511
                     /locus_tag="ACHKIR_004582"
     CDS             6107..6511
                     /locus_tag="ACHKIR_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlcG family protein"
                     /protein_id="HFO9992227.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKAAAAVLAK"
     gene            6533..8704
                     /gene="glcB"
                     /locus_tag="ACHKIR_004583"
     CDS             6533..8704
                     /gene="glcB"
                     /locus_tag="ACHKIR_004583"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HFO9992228.1"
                     /translation="MSQTITQGRLRINANFKRFVDEEVLPGTGLDTAAFWRNFDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPSCILLKNNGLHIELQIDANGRIGKDDSAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPHEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHQTNVQSVQANIAQTEFN
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKDQVQASLENMAKVVDQQNAGDPAYRPMVE
                     NFANSCAFKAACDLIFLGVKQPNGYTEPLLHAWRLREKENH"
     gene            9060..10742
                     /gene="glcA"
                     /locus_tag="ACHKIR_004584"
     CDS             9060..10742
                     /gene="glcA"
                     /locus_tag="ACHKIR_004584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate permease GlcA"
                     /protein_id="HFO9992229.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGAPVAITGALLVGLGFKPLYAAGLC
                     LIANTAPVAFGALGVPILVAGQVTGIDPFDIGAMAGRQLPFLSVLVPFWLVAMMDGWK
                     GVKETWPAALVAGGSFAVTQFFTSNYIGPELPDITSALVSIVSLSLFLKVWRPKNTET
                     AISMGQSAGAMVVNKPSSGGPVPSEYRLGQIIRAWSPFLILTVLVTIWTMKPFKALFA
                     PGGAFYSLVINFQIPHLHQQVLKAAPIVAQPTPMDAVFKFDPLSAGGTAIFIAAIISI
                     FILGVGIKKGIGVFAETLISLKWPILSIGMVLAFAFVTNYSGMSTTLALVLAGTGVMF
                     PFFSPFLGWLGVFLTGSDTSSNALFGSLQSTTAQQINVSDTLLVAANTSGGVTGKMIS
                     PQSIAVACAATGMVGRESELFRYTVKHSLIFASVIGIITLLQAYVFTGMLVS"
BASE COUNT         2496 a         2708 c         3048 g         2542 t
ORIGIN      
        1 ttgtggcgct tctgtgtagt ctctgacctt atcgtaatca ttaataaata ctatttattc
       61 tttgtttgta ttcattgggt tgattattaa gatgccgttc gttgctgttg caacctgttt
      121 ttcaccgtta attatttgtt atccacagag atatggaatg cgatctgctg atgattaaaa
      181 ttgaaaaagt aattaataaa aatgacctta aggcatttat cgcttttccg tcatcacttt
      241 atcccgatga tccaaactgg ataccccctt tattcattga acgcagcgag catttgtctg
      301 cgaagaatcc ggggacggac catatcatct ggcaggcgtg ggtggcaaaa aaagcggggc
      361 agatagttgg gcgtattacc gcgcaaatcg ataccttgca ccgtgagcgt tacggtgaag
      421 ataccggtca ttacggaatg attgatgcca ttgatgatcc gcaggttttt gccgcgttgt
      481 ttggcgcagc ggaagcgtgg ttaaagtcac aaggtgcaaa taagatcagc gggcctttca
      541 gcctgaatat caatcaggaa agcggattac tgattgaagg ttttgacact ccgccctgtg
      601 cgatgatgcc acacggcaaa ccgtggtatg ccgcgcatat tgaacaactg ggttatcaca
      661 aaggcattga tttactggcg tggtggatgc agcgaaccga tctcacgttc tctccggcgc
      721 taaaaaaact gatggatcag gtgcgcaaaa aagtgaccat tcgctgcatc aatcgccagc
      781 ggtttgccga agagatgcag atcctgcgtg agattttcaa ttctggctgg cagcacaact
      841 ggggatttgt gccgtttacc gaacatgaat tcgcgaccat gggcgatcaa cttaaatatc
      901 tggtgccgga cgatatgatc tacatcgctg agattgattc tgcaccctgc gcgtttattg
      961 ttggcttgcc gaacatcaac gaggcgattg ccgatctgaa cggatcgctc tttcccttcg
     1021 gctgggcaaa attgctgtgg cgtttgaaag tcagcggtgt gcgaactgcg cgagtgccgc
     1081 tgatgggcgt gcgggacgag tatcagttca gccgcatcgg cccggtaatt gcgctgttat
     1141 tgattgaagc cttacgcgat ccgtttgccc gtcggaagat cgatgcgctg gagatgtcat
     1201 ggatcctcga aaccaataca ggcatgaata acatgctgga acgcattggg gcggagccgt
     1261 acaagcgtta ccgattgtat gaaaagcaga tttgatggca gtcacccctg cttcatctcc
     1321 agcggcaccg ccgagcgaat caaatggtgg ccttccagct cgatatcgtg gagattcttt
     1381 ttcacggtcc gcacatgatc acgtgctgcg cgctgggcga cgtgcggcag ccgctgcaac
     1441 accgcgttgt agatccgcgc atgctggcga tcgatctgct ttttttgctg tggtcgatgg
     1501 tagagattat ttacgctggc aaacactgaa ttaaacatca gatcggtcaa tgattgcagc
     1561 gtaaacacca gcacctgatt gtgagaagcc tgacaaatgg cgagatggaa agcgtgatcc
     1621 aactgcgcat gttcgatcag cgaaatctct ttgttgttct cactggcggc gagcattttt
     1681 tcataacagc gggttatcac aacaaaatca gcctgggttc ccagcgttgc cgccagcctt
     1741 gccgattcgc cctccagtaa tgcgcgaacg tcgagcagat cgtacagcgt tcgcggctgc
     1801 gtactgaaca gatggatcag cgggctggcg tcctgcaccc gattaagccg tgcgacacga
     1861 gaatcgcgac cctgcgccgt ttcaataatc ccgcgcccgc gcagcacggt cagcccttca
     1921 cgcagtgcgg agcgtgagaa gccgagcttt tcacacagtc gacgttccga gggaagcggc
     1981 tgaccgacct tcaacacgcc gtcgataatt aaccgttcga tactctctgc aaccacttcg
     2041 caaatagggc gacgttcatc tttcattccc ggacctcgtg cacaggtagg accaattttt
     2101 ctgttcttaa ctcgcaaaac acgcacatca cgcaagtgat tatgttacat caatttaaca
     2161 ttaagttaac caaggcaagg tcacagagct ggaaaaaaaa tggtctgacc ggtagctaaa
     2221 gagatagacg aaaacgaaaa gcccgcttaa taactgttca cagaagcagc gcgcaaaaat
     2281 cagctgccac acaacacaac aaagcgaagc ctattcatga gcatcttgta cgaagagcgt
     2341 cttgatggcg ctttacccga tgtcgaccgc acatcggtac tgatggcact gcgtgagcat
     2401 gtccctggac ttgagatcct gcataccgat gaggagatca ttccttacga gtgtgacggg
     2461 ttgagcgcgt atcgcacgcg tccattactg gttgttttgc caaagcagat ggaacaggtg
     2521 acggcgattc tggctgtctg ccaccgcctg cgtgtaccgg tggtgacccg tggtgcaggc
     2581 accgggcttt ctggtggcgc gctgccactg gaaaaaggtg tgttgttggt gatggcgcgc
     2641 tttaaagaga tcctcgacat taaccccgtt ggtcgccgcg cgcgcgtgca gccgggcgtg
     2701 cgtaatctgg cgatctccca ggccgttgcg ccgcataatc tctattacgc gccggatcct
     2761 tcctcacaaa tcgcctgttc aattggcggc aatgtggcgg aaaatgccgg tggcgttcac
     2821 tgcctgaaat atggtctgac cgtacataac ctgctgaaaa ttgaagtgca aacgctggac
     2881 ggcgaggcgc tgacgctggg atcggatgcg ctggattcgc ctggttttga cctgctggcg
     2941 ctgttcaccg gatcggaagg tatgctcggc gtgaccaccg aagtgacggt aaaactgctg
     3001 ccgaagccgc ccgtggcgcg ggttctgtta gccagctttg actcggtaga aaaagccgga
     3061 cttgcggttg gtgacatcat cgccaatggc attatccccg gcgggctgga gatgatggat
     3121 aacctgtcga tccgcgcagc ggaagatttt attcatgccg gttatcccgt cgacgccgaa
     3181 gcgattttgt tatgcgagct ggacggcgtg gagtctgacg tacaggaaga ctgcgagcgg
     3241 gttaacgaca tcttgttgaa agcgggcgcg actgacgtcc gtctggcaca ggacgaagca
     3301 gagcgcgtac gtttctgggc cggtcgcaaa aatgcgttcc cggcggtagg acgtatctcc
     3361 ccggattact actgcatgga tggcaccatc ccgcgccgcg ccctgcctgg cgtactggaa
     3421 ggcattgccc gtttatcgca gcaatatgat ttacgcgttg ccaacgtctt tcatgccgga
     3481 gacggcaaca tgcacccgtt aatccttttc gatgccaacg aacccggtga atttgcccgc
     3541 gcggaagagc tgggcgggaa gatcctcgaa ctctgcgttg aagttggcgg cagcatcagt
     3601 ggggaacatg gcatcgggcg cgaaaaaatc aatcaaatgt gcgcccagtt caacagcgat
     3661 gaaatcacga ccttccatgc ggtcaaggcg gcgtttgatc ccgatggttt gctgaaccct
     3721 gggaaaaaca ttcccacgct acaccgctgt gctgaatttg gtgccatgca tgtgcatcac
     3781 ggtcatttac ctttccctga actggagcgt ttctgatgct acgcgagtgt gattacagtc
     3841 aggcgctgct ggagcaggtg aatcaggcga ttagcgataa aacgccgctg gtgattcagg
     3901 gaagcaatag caaagccttt ttaggccgcc ctgtcaccgg gcaaacgctg gatgtgcgtt
     3961 gtcatcgagg cattgttaat tacgacccga ccgagctggt gataaccgcg cgtgccggaa
     4021 cgccgctggt agccattgaa gcggcgctgg agagcgccgg gcaaatgctc ccctgcgagc
     4081 cgccgcatta tggtgaagaa gccacctggg gcgggatggt cgcctgcggg ctggcggggc
     4141 cgcgtcgccc gtggagcggt tcggtccgcg attttgtcct cggcacgcgc atcattaccg
     4201 gcactggaaa acatctgcgt tttggtggcg aagtgatgaa aaacgttgcc ggatacgatc
     4261 tctcacggtt aatggccgga agctacggtt gtctgggtgt gctcactgaa atctcgatga
     4321 aagtgttacc gcgaccacgc gccaccctga gtctgcgtcg ggaaatcagc ctgcaagaag
     4381 ccatgaatga aatcgcccag tggcaacttc agccattacc cattagtggc ttatgttact
     4441 tcgacaatgc gttgtggatc cgccttgagg gcggcgaagg atcggtaaaa gcagcgcgtg
     4501 aactgctggg gggcgaagag gttgcgggtc agttctggca gcaattgcgt gaacaacaac
     4561 tgccgttctt ctcgttacca ggtaccttat ggcgcatttc attacccagc gatgcgccga
     4621 tgatggattt acccggcgag caactgatcg actggggcgg ggcgttacgc tggctgaaat
     4681 cgacagccga tgacaatcaa atccatcgca tcgcccgcaa cgctggcggt catgcgaccc
     4741 gctttagtgc cggagatggt ggctttgccc cgctaccggc tcctttattc cgctatcacc
     4801 agcagcttaa acagcagctc gacccttgcg gcgtgtttaa ccccggtcgc atgtacgcgg
     4861 aactttgagg agcaggctat gcaaacccga ttaacagaag agatgcggca gaacgcgcgc
     4921 gcgctggaag ccgacagcat cctgcgcgcc tgtgttcact gcggattttg taccgcaacc
     4981 tgcccaacct atcagcttct gggcgatgaa ctggacgggc cgcgcgggcg catctatctg
     5041 attaaacagg tactggaagg caacgaagtc acgcttaaaa cacaggagca tctcgatcgc
     5101 tgtctcactt gccgcaattg tgaaaccacc tgtccttctg gtgtgcgcta tcacaatttg
     5161 ctggatatcg ggcgtgatat tgtcgagcag aaagtgaaac gcccactgcc ggagcgaatg
     5221 ctgcgcgaag gattgcgcca ggtagtgccg cgtccggcgg tcttccgtgc gctgacgcag
     5281 gtagggctgg tgctgcgacc gtttttaccg gaacaggtca gagcaaaact gcctgctgaa
     5341 acggtgaaag ctaaaccgcg tccgccgctg cgccataagc gtcgggtttt aatgctggaa
     5401 ggctgcgccc agcctacgct ttcgcccaac accaacgcgg caactgcgcg agtgctggat
     5461 cgtctgggga tcagcgtgac gccagctaac gaagcaggct gttgtggcgc ggtggactat
     5521 catcttaatg cgcaagagaa agggctggcg cgggcgcgca ataatattga tgcctggtgg
     5581 cccgcgattg aagcaggtgt cgaggcaatt ttgcaaaccg ccagcggttg cggcgcgttc
     5641 gttaaagagt atgggcagat gctgaaaaac gatgcgttgt atgccgataa agcgcgtctg
     5701 gtcagtgaac tggcggtcga tttagtcgaa cttctgcgcg aggaaccgct ggaaaaactg
     5761 gcaattcgcg gcgataaaaa actggccttc cactgcccgt gtaccctaca acatgcgcaa
     5821 aagctgaacg gcgaagtgga aaaagtgtta cttcgtcttg gatttacctt aacggacgtt
     5881 cccgacagcc atctgtgctg cggttcagcg ggaacatatg cgttaacgca tcccgatctg
     5941 gcacgccagc tgcgggataa caaaatgaat gcgctggaaa gcggcaaacc ggaaatgatc
     6001 gtcaccgcca acattggttg ccagacgcat ctggcgagcg ccgatcgtac ctctgtgcgt
     6061 cactggattg aaattgtaga acaagccctt gaaaaggaat aacaaaatga aaactaaagt
     6121 cattcttagc cagcaaatgg cgagtgcaat tattgccgca ggtcaggaag aggcgcagaa
     6181 aaataactgg tctgtttcca ttgctgttgc cgatgacggc ggtcatctgc tggcgttaag
     6241 tcgcatggac gattgcgcgc cgattgcggc ttacatctcc caggagaaag cgcgtaccgc
     6301 cgcgctgggg cgtcgtgaaa ctaagggcta tgaagagatg gtgaacaacg gacgtaccgc
     6361 gttcgtgact gcgccgttac tgacgtcgct ggaaggtggc gtaccggttg ttgtggatgg
     6421 gcaaattatt ggtgccgtgg gcgtttctgg tttaaccgga gcacaggatg cgcaggtcgc
     6481 gaaagcggca gcagcggtgt tggcgaaata agcgaaaacg aggagataaa caatgagtca
     6541 aaccataacc cagggccgtt tacgcattaa cgccaatttt aaacgttttg tggatgaaga
     6601 agttttaccg ggaacagggt tggacactgc ggcgttctgg cgcaattttg atgagatcgt
     6661 tcacgatctg gcaccagaaa atcgtcagtt gctggcagaa cgcgatcgca ttcaggcggc
     6721 gcttgatgag tggcatcgca gcaatccggg gccggtaaaa gataaagcgg cctataaatc
     6781 tttcctgcgt gaactgggct acctggtgcc gcaaccggag cgcgtgacgg tggaaaccac
     6841 gggcattgac agcgaaatca ccagccaggc ggggccacag ctggtggttc cggcaatgaa
     6901 cgcccgctac gcgctgaacg cggcgaacgc tcgctggggc tcactgtacg atgcgttata
     6961 cggcagcgac atcatcccgc aggaaggggc gatggtcagc ggctacgatc cgcaacgcgg
     7021 tgagcaggtt atcgcctggg ttcggcgttt cctcgatgaa tcactaccgc tggaaaacgg
     7081 cagctatcag gatgtggtgg cgtttaaggt ggtcgataaa caattacgca tccagttgaa
     7141 aaatggtaaa gaaaccacgt tacgtacccc ggcgcagttt gtcggttacc gtggcgatgc
     7201 cgctgcgccg tcctgcattt tgctgaaaaa taacggcctg catattgagc tacaaatcga
     7261 tgccaacggg cggattggca aagacgattc ggcgcacatc aacgatgtta tcgtcgaagc
     7321 ggccatcagt accattctcg actgcgaaga ttcggttgcg gcggttgatg cggaagataa
     7381 aatcctgctg taccgcaatc tgctgggtct gatgcagggg actctgcaag agaaaatgga
     7441 gaaaaacggt cggcaaatcg tgcgtaaact gaatgacgat cgtcagtaca ccgctgccga
     7501 cggctctgaa atttctctgc acgggcgttc gctgctgttt atccgcaacg tgggtcattt
     7561 gatgaccatt cctgtgattt gggacagcga aggcaatgaa atcccggaag gcattcttga
     7621 tggcgtcatg actggcgcga ttgccctcta tgatttaaaa gtgcagaaaa actcgcgcac
     7681 tggcagcgtc tatattgtga aaccgaaaat gcacggtccg catgaagtgg cgttcgccaa
     7741 caaactgttt acccgcattg agacaatgct cggtatggca ccgaataccc tgaaaatggg
     7801 cattatggat gaagaacgtc ggacctcgct gaacttgcgt agctgtatcg ctcaggcgcg
     7861 taaccgcgtg gcgttcatca ataccggttt cctcgaccgt accggcgatg aaatgcattc
     7921 ggtgatggaa gctggcccga tgctgcgtaa aaaccagatg aaatcgacgc cgtggatcaa
     7981 agcctacgaa cgtaataacg tgctttccgg tctgttctgt gggctgcgcg gtaaagcgca
     8041 aatcggtaaa ggcatgtggg caatgccgga cctgatggca gacatgtaca gccagaaggg
     8101 cgaccagctg cgtgccgggg caaacacggc ctgggttccg tcgccaactg ccgctacgct
     8161 ccatgcgctg cactaccacc aaaccaacgt acagagcgtg caagccaaca ttgcccagac
     8221 cgagttcaat gctgaatttg aaccgctgct ggacgatctg ctgactattc cggttgctga
     8281 aaacgctaat tggtcggcgc aagagatcca acaagagctg gataacaacg tgcaggggat
     8341 tctggggtac gtggtgcgtt gggtggagca ggggattggt tgttcaaaag tgccggatat
     8401 tcacaacgtg gcgttgatgg aagaccgcgc aacgctgcgt atttccagcc agcatatcgc
     8461 caactggtta cgtcacggta ttctgaccaa agatcaggtg caggcgtcgc tggagaatat
     8521 ggcgaaagtg gttgatcagc aaaacgctgg cgatccggct tatcgtccaa tggtggagaa
     8581 tttcgctaac tcgtgtgctt ttaaagctgc ctgcgatttg atcttcctcg gagtgaaaca
     8641 gccaaacggc tataccgaac cgttattaca cgcatggcgt ttacgcgaaa aagaaaatca
     8701 ttaatagcgt caaaaaaagc cccggcaaga tttattgtcg gggctatgat agtaactgcg
     8761 gtagtacatt tatcccggaa ataaccggag cgattatcag gggttaacaa tattaaatac
     8821 ccgtagcgca tcactagcgg gattaaaaca acaatatgga catcttaacg atactctgcg
     8881 cctgcgtgtg cggaacagaa acccttaccc gatatacttg ttatgccaga ggtagcaatt
     8941 gcgttagcgg cgtactgttg ctgtaaagta taacgcctat attttacagt tcagtcctct
     9001 tttgcttatt acaaccttaa gtatatcaag catataaaga taataagaga ctgaacaata
     9061 tggttacctg gacccaaatg tatatgccga tgggaggact ggggctatcc gctctggtcg
     9121 ccctgatccc aataatattc ttcttcgttg cactcgcggt attacgtctg aaaggacatg
     9181 tcgctggagc aataaccctt atattatcta ttctgattgc gatattcgcc tttaaaatgc
     9241 cgattgatat ggcatttgct gctgcgggct atggctttat ttatggttta tggcctattg
     9301 cgtggattat tgtcgcggcg gtattcctgt ataaattaac cgttgccagc ggccagttcg
     9361 atattatccg cagctcggtt atctccatca ccgacgatca gcgtttgcag gtgttactga
     9421 ttggtttctc ctttggtgcg ctgctggaag gggcggctgg ctttggcgca ccggtggcga
     9481 ttaccggtgc tctactggtg ggcctgggct tcaaaccgct atacgcggcg gggctgtgtc
     9541 tgattgccaa cactgcgccg gtggcgtttg gtgcgttggg tgtgccaatt ctggtcgccg
     9601 gtcaggtaac gggaatcgat ccgttcgaca ttggcgcaat ggcgggacgt cagttaccgt
     9661 tcctgtcggt tcttgtgccg ttctggctgg tagcaatgat ggacggctgg aaaggggtga
     9721 aagagacgtg gccagcggcg ctggttgcag ggggaagctt cgctgtcact cagttcttta
     9781 cctctaacta tattggtccg gaactgccgg atattacttc ggcgctggtg agtatcgtct
     9841 cactttcttt atttcttaaa gtctggcggc caaaaaatac cgaaacggca atcagtatgg
     9901 gacaatccgc aggtgcgatg gtggtgaata aaccgtcttc tggcggtccc gtgccttcag
     9961 aatatcgtct ggggcaaatc attcgtgcgt ggtcaccgtt tttaatctta acggtgttgg
    10021 tcaccatctg gaccatgaag ccgtttaaag cgttatttgc tccgggcggc gcgttttatt
    10081 cgctggtgat taatttccag atccctcatt tgcatcaaca agtgttgaaa gcggccccca
    10141 ttgtcgccca gccgacgcca atggacgcgg tgtttaaatt cgaccccctc tctgctggtg
    10201 gcaccgctat ttttattgcg gcgattatct cgatcttcat cctcggcgtg gggatcaaga
    10261 aaggtattgg tgtctttgcc gaaacgctaa ttagtctgaa gtggccgata ctgtcgattg
    10321 gcatggtgct ggcatttgcc ttcgtgacca actattccgg catgtccacc acgctggcgc
    10381 tggtactggc tggtacaggc gtaatgttcc cgtttttctc accgtttctc ggctggctgg
    10441 gcgtattcct taccggctcg gacacctcct ctaacgccct gtttggttca ctgcaatcga
    10501 ccacggcgca gcaaatcaac gtctctgaca ccctgctggt ggcagcaaat accagcggcg
    10561 gcgtaactgg caagatgatc tccccgcaat ctatcgccgt ggcctgcgcc gcgacgggca
    10621 tggtgggccg agaatctgaa ctgttccgct acaccgtgaa gcacagtctg atttttgcca
    10681 gcgttatcgg cattatcacg ctgttgcagg cgtatgtgtt taccgggatg ttagtttcgt
    10741 aagtcaataa tgccggatga tcgttaatat catccggcta catttacatt attc
//
LOCUS       DAZGQL010000140        10767 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.140, whole genome shotgun sequence.
ACCESSION   DAZGQL010000140 DAZGQL010000000
VERSION     DAZGQL010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10767
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.140"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            42..95
                     /gene="ychE"
                     /locus_tag="ACHKIR_004585"
     CDS             42..95
                     /gene="ychE"
                     /locus_tag="ACHKIR_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YchT"
                     /protein_id="HFO9992230.1"
                     /translation="MLSKGVLSARLFNLIYG"
     gene            126..773
                     /gene="ychE"
                     /locus_tag="ACHKIR_004586"
     CDS             126..773
                     /gene="ychE"
                     /locus_tag="ACHKIR_004586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HFO9992231.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            complement(931..1227)
                     /locus_tag="ACHKIR_004587"
     CDS             complement(931..1227)
                     /locus_tag="ACHKIR_004587"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992232.1"
                     /translation="MRFLLANTFCKIVKQFSFIDRIRIYSLHPCLNKHLSIQSNRDWL
                     SMSLFSDYSSSSEMHNNLTIDYYLALSSTKGSGITNIISIILQQAQDYDVAKIT"
     gene            1511..3142
                     /gene="oppA"
                     /locus_tag="ACHKIR_004588"
     CDS             1511..3142
                     /gene="oppA"
                     /locus_tag="ACHKIR_004588"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HFO9992233.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIITNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKATDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            3228..4148
                     /gene="oppB"
                     /locus_tag="ACHKIR_004589"
     CDS             3228..4148
                     /gene="oppB"
                     /locus_tag="ACHKIR_004589"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HFO9992234.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            4163..5071
                     /gene="oppC"
                     /locus_tag="ACHKIR_004590"
     CDS             4163..5071
                     /gene="oppC"
                     /locus_tag="ACHKIR_004590"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HFO9992235.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            5083..6096
                     /gene="oppD"
                     /locus_tag="ACHKIR_004591"
     CDS             5083..6096
                     /gene="oppD"
                     /locus_tag="ACHKIR_004591"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppD"
                     /protein_id="HFO9992236.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNRLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            6093..7097
                     /gene="oppF"
                     /locus_tag="ACHKIR_004592"
     CDS             6093..7097
                     /gene="oppF"
                     /locus_tag="ACHKIR_004592"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppF"
                     /protein_id="HFO9992237.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(7150..7479)
                     /gene="yciU"
                     /locus_tag="ACHKIR_004593"
     CDS             complement(7150..7479)
                     /gene="yciU"
                     /locus_tag="ACHKIR_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1450 family dsDNA-mimic protein"
                     /protein_id="HFO9992238.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(7514..8974)
                     /gene="cls"
                     /locus_tag="ACHKIR_004594"
     CDS             complement(7514..8974)
                     /gene="cls"
                     /locus_tag="ACHKIR_004594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFO9992239.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            9117..9290
                     /gene="yciY"
                     /locus_tag="ACHKIR_004595"
     CDS             9117..9290
                     /gene="yciY"
                     /locus_tag="ACHKIR_004595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HFO9992240.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(9345..10598)
                     /gene="kch"
                     /locus_tag="ACHKIR_004596"
     CDS             complement(9345..10598)
                     /gene="kch"
                     /locus_tag="ACHKIR_004596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HFO9992241.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
BASE COUNT         2792 a         2495 c         2671 g         2809 t
ORIGIN      
        1 ataacgaatg atagcaattt taagtagtta ggaggtgaaa aatgctgtca aaaggcgtat
       61 tgtcagcgcg tctttttaac cttatttatg gctaacatta tccggctttt gcttcggagc
      121 taaccgtgat tcagaccctt tttgattttc ccgtttactt caaatttttc atcgggttat
      181 ttgcgctggt caacccggta gggattattc ccgtctttat cagcatgacc agttatcaga
      241 cagcggcagc gcgaaacaaa acgaacctta cagccaacct gtctgtggcc attatcttgt
      301 ggatctcgct ttttctcggc gacacgattc tacaactttt tggtatatca attgattcat
      361 tccgtatcgc cggtggcatc ctggtggtga cgatagcgat gtcgatgatc agcggcaagc
      421 ttggcgagga taaacagaac aagcaagaaa aatcagaaac cgcggtgcgt gaaagcattg
      481 gtgtggtgcc attggcgttg ccgttgatgg cggggccagg ggcgatcagt tctaccatcg
      541 tctgggggac gcgttatcac agcattagct atctgtttgg tttctttgtg gctattgcat
      601 tgttcgcttt atgttgctgg ggattgttcc gcatggcacc gtggctggta cgggttttac
      661 gccagaccgg catcaacgtg attacgcgta ttatggggct attgctgatg gcattgggga
      721 ttgaatttat cgttactggt attaagggga ttttccccgg cctgcttaat taattccttt
      781 caaatgaaac ggagctgtca tgctccgttt acttcgtcat tatttttact ttgttcccgc
      841 gcagttatca aaagcaaaag gaatgggtga aaatattctt ctcaaattac agttagttat
      901 aaggatttcc ttaactgctt ctcctcacca tcatgttatt ttcgccacat cataatcctg
      961 ggcttgctga agaataattg aaatgatatt agtaattcca ctgcctttgg tagaggaaag
     1021 tgctaaataa taatcaattg ttaaattatt gtgcatttca ctactggaac tgtaatcaga
     1081 aaagagagac atgcttaacc aatctctatt tgattgaatt gaaagatgtt tgttaagaca
     1141 tggatgcaag ctatagattc tgatgcggtc aataaaagag aattgcttaa caattttgca
     1201 aaatgtattg gcgagtaaga agcgcatttg gtactttccg ggcaaccgcc aggcgattct
     1261 ttattggtaa tgagaataat taacaattaa agagcgtcgc gaaagaataa tgtgtctcga
     1321 caggggagac acagtacgaa tcgacataag gtgatcgtct gaatcaccag aataaataaa
     1381 gtcggtgata gcaatccgta acgataaagt aacctgacag cagaaagtct ccgagcctgt
     1441 gcagggtccc aatccgggat tacacatgct ggttaatacc agtaattata atgagggagt
     1501 ccaaaaaaca atgaccaaca tcaccaagag aagtttagta gcagctggcg ttctggctgc
     1561 actaatggca gggaatgtcg cgctggcagc tgatgtaccc gcaggcgtca cactggcgga
     1621 aaaacaaacg ctggtacgta acaatggttc agaagttcag tcattagatc cgcataaaat
     1681 tgaaggtgtt ccggagtcta atatcagccg agacctgttt gaaggcttac tggtcagcga
     1741 tcttgacggt catccggcac ctggcgtcgc tgaatcctgg gataataaag acgcgaaagt
     1801 ctggaccttc catttgcgta aagatgcgaa atggtctgat ggcacgccag tcacagcaca
     1861 agactttgtg tatagctggc aacgttctgt tgatccaaac accgcttctc cgtatgccag
     1921 ttatctgcaa tatgggcata tcgccggtat tgatgaaatt cttgaaggga aaaaaccgat
     1981 taccgatctc ggcgtgaaag caattgatga tcacacatta gaagtcacct taagtgaacc
     2041 tgttccgtac ttctataaat tacttgttca cccatcaact tcaccggtgc caaaagccgc
     2101 tatcgagaaa ttcggcgaaa aatggaccca gcctggtaat atcatcacca acggtgccta
     2161 taccttaaaa gattgggtcg taaacgaacg aatcgttctt gaacgcagcc caacctactg
     2221 gaacaacgcg aaaaccgtta ttaaccaggt aacctatttg cctattgctt ctgaagttac
     2281 cgatgtcaac cgctaccgta gtggtgaaat cgacatgacg tataacaaca tgccgatcga
     2341 attgttccag aagctgaaaa aagagatccc ggacgaagtt cacgttgatc catacctgtg
     2401 cacttattat tacgaaatta acaaccagaa accgccattc aacgatgtac gtgtgcgtac
     2461 cgcactgaaa ctgggtatgg accgcgatat cattgttaat aaagtgaaag cgcagggcga
     2521 catgcccgcc tatggttaca ctccaccgta tactgatggc gcaaaattga ctcagccgga
     2581 atggtttggc tggagccagg aaaaacgtaa cgaagaagcg aaaaaactgc tggctgaagc
     2641 gggttatacc gcagacaaac cgttgaccat caacctgttg tataacacct ccgatctgca
     2701 taaaaagctg gcgattgctg cctcttcatt gtggaagaaa aacattggtg taaacgtcaa
     2761 actggttaac caggagtgga aaacgttcct cgacacccgt caccagggca cttttgatgt
     2821 ggcccgtgca ggctggtgtg cagactacaa cgaaccaact tccttcctga acaccatgct
     2881 gtcgaacagc tcaatgaata ccgcgcatta taagagcccg gcctttgaca gcattatggc
     2941 ggaaacgctg aaagcgactg acgaggcgca gcgcacagct ctgtacacta aagcagaaca
     3001 acagctggat aaggattcgg ccattgttcc tgtttattac tacgtgaatg cgcgtctggt
     3061 gaaaccgtgg gttggtggct ataccggcaa agatccgctg gataatacct acacccggaa
     3121 tatgtacatt gtgaagcact aatggcaata cgtggggcag gcgtgtactg ctccacggtg
     3181 tctgattttt atcgcattac agaaggcaca ggccagaagg tagggcaatg ttaaaattta
     3241 ttctacgtcg ctgtctggaa gcgattccga cgctatttat tcttattact atttcgttct
     3301 tcatgatgcg cctcgcgccg ggaagccctt ttaccggcga acgtacttta ccgccagaag
     3361 tgatggccaa tatcgaagcg aaatatcatc ttaatgatcc gatcatgaca cagtatttca
     3421 gctacctgaa acaactggcg catggcgatt tcggtccatc gtttaaatat aaagattatt
     3481 cggtcaacga cctggttgca tccagttttc ccgtttctgc caaactggga gccgcagcat
     3541 ttttccttgc ggtaatactg ggtgttagtg ctggcgttat tgccgcatta aaacaaaaca
     3601 ccaaatggga ctataccgtg atggggctgg caatgaccgg ggttgttatc cccagttttg
     3661 tggttgcgcc attattagtc atgatatttg cgatcatttt gcattggctg ccgggcggtg
     3721 gctggaatgg tggtgcgctt aaattcatga tactgccaat ggtggcgttg tcactcgcct
     3781 atatcgccag tattgcgcgt attacccgtg gctctatgat tgaagtatta cactccaact
     3841 ttattcgtac tgcccgggcg aaagggttac ctatgcggcg gatcatttta cgccacgcat
     3901 taaaacctgc tctgttaccc gtgctctcct atatgggccc cgcatttgtc ggcattatta
     3961 ccggttctat ggtcatcgaa accatttatg gtttgccggg gattgggcaa ctgttcgtta
     4021 atggcgcatt gaaccgtgac tattccttag tgttaagcct gactatcctg gttggtgcgt
     4081 taaccatttt gtttaatgcc attgtcgatg tgctatatgc ggttatcgac ccgaaaatcc
     4141 gttactgata ctggagctcg cgatgatgtt aagtaagaaa aacagcgaga cgctggaaaa
     4201 tttcagtgaa aagctggagg tcgaagggcg cagcttgtgg caggacgcac gtcgacgttt
     4261 tatgcataac cgtgcggcgg ttgccagtct gatagtgctg gtgctaatcg cgttatttgt
     4321 aatcctggca ccgatgcttt cgcagtttgc ctatgacgat actgactggg cgatgatgtc
     4381 cagcgccccg gatatggagt ccggtcacta ctttggtact gactcatccg gtcgcgacct
     4441 gcttgtgcgt gttgcgattg gcgggcgtat ctcactcatg gtcggtgttg ctgcggcact
     4501 ggtggcagtg gtcgtgggga cactttatgg ttcgctttcc ggttatctgg gcggtaaagt
     4561 ggattcggta atgatgcgtc tgctggaaat cctcaactcc ttcccattca tgttcttcgt
     4621 cattttgctg gtgacctttt tcggtcaaaa catcctgctg attttcgtgg cgattggcat
     4681 ggtttcctgg ctggatatgg ctcgtattgt gcgtgggcaa accctgagtc tgaagcgcaa
     4741 agagtttatt gaggcggcac aagttggcgg tgtatcgacg tcgggcattg ttattcgcca
     4801 cattgtgccg aatgtactcg gtgtggtggt ggtctacgca tcacttctgg tgcccagcat
     4861 gatcctcttt gaatctttcc ttagcttcct ggggttgggg acgcaagagc cgttaagcag
     4921 ctggggcgca ttgctgagtg atggcgcgaa ctcgatggaa gtctctccat ggttactgtt
     4981 gttcccagcg ggattcctcg tggtgacgct attttgtttc aactttatag gcgatggctt
     5041 gcgtgatgcc ctcgacccga aagatcgtta aggagtgcag ccatgagcgt aattgaaact
     5101 gcaactgtgc cgctcgcaca acaacaggct gacgcactgc tgaacgtgaa agatttgcgt
     5161 gtcaccttta gtaccccgga cggcgacgtc acggcggtca atgatttgaa tttttcccta
     5221 cgtgccggag aaacgctggg cattgtaggt gagtctggtt cgggtaaatc gcaaactgca
     5281 tttgcgttga tgggcctgct ggctgccaac ggacgtattg gcggatcggc aaccttcaat
     5341 gggcgtgaaa tcctcaattt gccagagcgt gaactcaata gactgcgcgc tgaacaaatc
     5401 tcaatgattt ttcaggaccc aatgacttcg ttgaatccct atatgcgcgt cggtgagcag
     5461 ttgatggaag tgctgatgct gcataagaac atgagcaaag ctgaggcgtt tgaagagtcg
     5521 gtgcggatgc tcgatgcggt aaaaatgccg gaagcacgta aacgcatgaa aatgtacccg
     5581 catgaatttt ctggtggcat gcgtcagcga gtcatgattg cgatggcatt gctatgtcga
     5641 cctaagctgc tgattgcgga tgaacccact actgcgctgg acgtcaccgt acaggcgcag
     5701 atcatgacgc tattaaatga actgaagcgg gaatttaata ccgccatcat tatgatcacc
     5761 cacgatcttg gcgtggtggc ggggatctgc gacaaagtgc tggtaatgta cgccgggcgc
     5821 acgatggaat atggcaacgc gcgcgatgtc ttttatcaac ccgttcatcc ttattctatc
     5881 ggtttgctca acgcggtgcc gcgtctcgat gcggaaggtg aaacaatgtt gaccatccct
     5941 ggtaatccgc caaacctgct gcgattaccg aaaggttgcc cgttccagcc acgttgtccg
     6001 catgcgatgg aaatttgtag tagcgcaccg ccgctggaag agtttacgcc tggccgtctg
     6061 cgtgcttgct ttaaaccggt ggaggaactg ttatgaatgc tgtaaccgaa ggaagaaaag
     6121 tcctcctcga aatcgccgat ctgaaagtgc actttgaaat caaagatggc aaacagtggt
     6181 tctggcaacc gccgaaaacg ctcaaagccg tcgatggtgt cactcttcgc ctgtatgaag
     6241 gggaaacatt aggtgtggta ggggaatcgg gatgcggtaa gtccaccttt gctcgcgcca
     6301 tcattggttt ggtcaaggcg accgacggtc atgttgcctg gttaggtaaa gagttgctgg
     6361 gcatgaagcc cgatgaatgg cgtgccgttc gcagtgatat tcagatgatt ttccaggatc
     6421 cattggcatc gcttaacccg cgtatgacca tcggcgagat catcgctgaa ccactgcgta
     6481 cttatcatcc gaaaatgtca cgccaggaag ttcgcgagcg cgtgaaggcg atgatgctga
     6541 aagtcgggtt attgcctaac ctgattaacc gctatccgca tgagttctct ggtgggcagt
     6601 gccagcgtat cgggattgct cgtgctctta ttcttgaacc gaagctgatt atctgtgatg
     6661 agccggtgtc ggcgctggac gtgtcaattc aggcgcaggt ggtcaacctg ctccagcagc
     6721 tgcaacgcga gatgggattg tcattaattt ttatcgctca tgacctggcc gtggtaaaac
     6781 acatttccga tcgtgtgctg gtgatgtatc tcggccatgc ggtagaactg gggacctatg
     6841 atgaggtcta tcacaatccg ctacatcctt acaccagggc attgatgtcg gcagtcccca
     6901 tacctgatcc ggatctggag aagaacaaaa ccatccagtt actggaaggg gaattaccgt
     6961 cgccgatcaa cccgccttcc ggttgtgttt tccgtacccg ttgcccgatt gccggtccag
     7021 agtgtgccaa aacacgtcct gtgctggagg ggagtttcag acacgccgtt tcttgcctga
     7081 aagtcgatcc gctttaaaag caataagggc tgacagttgt cagcccttat tcacgccaaa
     7141 agcgattatt tattcccgcc agatgatatg gcacagtttg tgatcttttt cgcggcataa
     7201 cagaatgcgc gcgaaaacat cgttgatttc accatcttca ctgtccgcca gaccaatcac
     7261 cacttcggca aaaaagtccg ggttcaaatc gaaatcaaca tgttcctgcc agtcttctgc
     7321 cggatcgaat aactccgcgc cgccgcgctc ttcaaactga agattgaaca gcagaacatc
     7381 agctggatcg agattgtcgg cagccagttc gagaaaaata tcgtaggcct gctcaagcgt
     7441 ttcatcttca gtcaggcgat tgtttagatc catatccata gtcactacct gtttaacctc
     7501 tgttggcgac gttttacagc aacgggctga agaagtaaaa cagtcgctcg gcgacacgtt
     7561 gccataatgg acgttttagc cataaacggg catcgagcag acgtgaacgc gaaatataat
     7621 cgtcctgaac ggcggcaagg tctgcaccaa aacctttatc gtcgattgcc agggtaatct
     7681 cgaaatttag ccacagactc cgcatatcaa ggttaacggt gccaaccaga cttagttcac
     7741 catcgaccag cacgctcttg gtatgcagta acccgccttc aaactgataa attttaaccc
     7801 cagcagccag cagttccgta aagaatgcgc gactggccca gccgaccagc atcgagtcat
     7861 tttttcgcgg aaggataata ctgacatcca ccccgcgctg cgccgccgtg caaatcgcat
     7921 gaagtaaatc atcgctgggc acaaagtagg gcgttgtcat gatcaaatat tcacgcgccg
     7981 aataagcagc agtcaataat gcctggtgaa tgagatcttc cggaaagccg gggccagaag
     8041 caattgtgtg aatggtgtga ccgctggcct gctcaaacgg cataatattg acatctggtg
     8101 gtggcggcag aatacgtttt ccggtttcaa tctcccagtc gcaggaataa ataatcccca
     8161 tcgcggtagc gatgggacct tccatacgcg ccatcagatc aatccattgc cctacgcccg
     8221 catcttgttt gaagtagcga gggtcgacca tattcatgct gccggtgtac gcgatgtaat
     8281 tatcgatcat gatcatcttg cgatgttggc gcaggtccat acggcgtaaa aacacacgca
     8341 tcagattgac ctttaaggct tcgacaactt caataccggc attacgcatt agctcgggcc
     8401 acgggctgcg gaaaaaagcc acactcccgg cggagtcgag catcaatcgg caatgaatgc
     8461 cgcgtcgcgc agccgccatt aatgattcag ccacctggtc cgccatgccg ccgggctgcc
     8521 agatataaaa caccatctca atattatggc gagcgagctg gatgtcgcgg attaacgcct
     8581 gcatcacatc atctgactcg gtcatcagtt gcagctgatt ccctttgacc ccagcgatcc
     8641 cctgacgacg ctcgcaaagc ttgaataatg gcgcagcgac actgctattt tcttcggcga
     8701 agatatgctt acaggcttta aggtcgttaa gccattttgc ggtggaaggc cacatcgctc
     8761 tggcgcgctc agcgcggcgt ttgcctaaat ggagctcgcc aacggcaaga taggcaataa
     8821 ttccgactaa cggcagaatg taaataatca acagccaggc catcgcggag ggaactgcgc
     8881 gtcgtttcat tagaatgcgt aaagttacgc ctgcaatgag caaccagtat cccagaatgg
     8941 ccaaccaact caccaacgta taaacggttg tcatagatta gaaatccttt tgaaagcgca
     9001 ttgttatgag tttacgcata tggattcatc tggcaaataa aaacgcggga aaagcactgg
     9061 tcagcgtcgg gggcgggggt ataatgacca ttctgttatt gcatagagta gttaacatga
     9121 agcggagtag aacggaagtg gggcgctggc gcatgcagcg tcaggctagc cgacgtaaat
     9181 cgcgttggct tgaggggcag tcgcgccgaa atatgcgtat ccacagcatc aggaagtgca
     9241 ttctaaacaa acagcgtaac tcgttattgt ttgcgatcta caatatctaa atgtactggg
     9301 caccattgcg gtgcccaata agtttactct tccctcttta aactctattt ttgcgccgat
     9361 tctttactgt cagctttcgt ttcttgttca tcgttatcgc tgaaaatgcc atgaccggag
     9421 ttcaacaaca ttgaaacgag catatcatta ttaatctctt caccatttaa aactcgcgcc
     9481 agaatttcgc tgccaaacag ttgcggcgag agaatgatat ccggatgtac catcttaatc
     9541 ttattcaggt ttttgctatc actgacggcg agaactgttt tgacatcact gctcatatct
     9601 tttgccgaga gtacaacaaa cgcgttatct gcatcgttat cactcagcgc cagaatggcc
     9661 cggcatcgat caattccagc tttctttaat actgaactgt cattactgtc accggggata
     9721 acatcagcgt tatcgcctaa gcgttgctca agttgcttga tatcatcttc aggcaagttg
     9781 ctgataaccg ttacgttttg tccgcgttga ttcagttgca ggatcgtatt gatggcgaga
     9841 atcgaatgtc cgcaaacaat aaaatgatct ttacgatgca ttgtatgatt gtttcctttt
     9901 acaagtttgt tgaatccccc gcggataagc ggaccaaaaa ttgaggtcat agatgtggca
     9961 aaaacggtaa tgccggaaat aatgaccgaa atagtgaaca atcgtgctga ttcagaaaca
    10021 gggacaatat cgccatagcc gacggttgac atggtttcta tcgaaaaata gaacgcggtc
    10081 atcaaacttt ctattcgcgg attaaaacct tcgcttaaat aaagcgcacc gtaggtggag
    10141 taaaacagta acgtcgtgaa actaataaat gcgaaaattg tcccggctgc ggcgctactg
    10201 tgggagaagt ctttgcgcag tatcagcaaa aacaccagcg taaaaatgca aaatccaata
    10261 ctaaatttca gccagggata aaaatgcagg gtgtaaatta gcgctatcaa caacaaaatg
    10321 atactgattg cccatgcgag ctttgcccgg aacagtaagc caatggagtt caggacaaga
    10381 aagacgccga gcataaagag tggggcgttt gccagcaagg agagatccaa ctctgaaaat
    10441 gctttgatat ggaaaatatc tagcaaattg accgacatac catagattgt tttaaaaata
    10501 agcaatccat ttaaaaagac ggccagcgcg agaatgtcgt gccgtagggt cacccataaa
    10561 ttagttgctg tttgtttgaa tgtagcccag tgactcactg atacctctgt aaccacaatt
    10621 ttgtttaatt atcttagcgt gcacattttc tgaacataca tgcagcgcgt gaatgtgtta
    10681 aacaatagta gatgacttta tccgcagtaa tcaaaggttt tgagtgagca actctgcgtc
    10741 aggaaagggc atttttcctg aatatgc
//
LOCUS       DAZGQL010000141        10762 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.141, whole genome shotgun sequence.
ACCESSION   DAZGQL010000141 DAZGQL010000000
VERSION     DAZGQL010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10762
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.141"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            288..1319
                     /gene="galR"
                     /locus_tag="ACHKIR_004597"
     CDS             288..1319
                     /gene="galR"
                     /locus_tag="ACHKIR_004597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HFO9992242.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLVVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(1326..2588)
                     /gene="lysA"
                     /locus_tag="ACHKIR_004598"
     CDS             complement(1326..2588)
                     /gene="lysA"
                     /locus_tag="ACHKIR_004598"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFO9992243.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVLEFGQD
                     LQAISAGGGLSVPYQQGEEAVDTEHYYGLWNAARQQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            2710..3645
                     /gene="lysR"
                     /locus_tag="ACHKIR_004599"
     CDS             2710..3645
                     /gene="lysR"
                     /locus_tag="ACHKIR_004599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HFO9992244.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFHGENYISLSRTDSYRQLLDQLFTENQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(3632..4324)
                     /gene="ygeA"
                     /locus_tag="ACHKIR_004600"
     CDS             complement(3632..4324)
                     /gene="ygeA"
                     /locus_tag="ACHKIR_004600"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HFO9992245.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCSLPFL
                     HIADATGRAIIRAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADKRAKINQI
                     IFEELCLGQFTEASRTYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(4453..5871)
                     /gene="araE"
                     /locus_tag="ACHKIR_004601"
     CDS             complement(4453..5871)
                     /gene="araE"
                     /locus_tag="ACHKIR_004601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HFO9992246.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(6186..6947)
                     /gene="kduD"
                     /locus_tag="ACHKIR_004602"
     CDS             complement(6186..6947)
                     /gene="kduD"
                     /locus_tag="ACHKIR_004602"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HFO9992247.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIKQVTALGRRFLSLTADLRKIDGIPALLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(6977..7813)
                     /gene="kduI"
                     /locus_tag="ACHKIR_004603"
     CDS             complement(6977..7813)
                     /gene="kduI"
                     /locus_tag="ACHKIR_004603"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFO9992248.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPVTKTVSVGGEVGKQLGVSYFLERRELGIINIGGAGTITVDGQCYEIGHRE
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(8100..9281)
                     /gene="yqeF"
                     /locus_tag="ACHKIR_004604"
     CDS             complement(8100..9281)
                     /gene="yqeF"
                     /locus_tag="ACHKIR_004604"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HFO9992249.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMTQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            9536..>10762
                     /gene="yqeG"
                     /locus_tag="ACHKIR_004605"
     CDS             9536..>10762
                     /gene="yqeG"
                     /locus_tag="ACHKIR_004605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO9992250.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
BASE COUNT         2778 a         2846 c         2487 g         2651 t
ORIGIN      
        1 cctgcgcatc tgcgcaggct ggtgtaattc atgtgctcaa cccgaagttg acttcaccta
       61 tcaatacctc tgggatcacc actttagcaa cctgaagcca aacgccacca gcggtcaatc
      121 gcaacagcgt ttcgcaaagt gtaaccaaag gtttgcaatc tctttttttg tcttacttat
      181 ttgcgtttgg ctcacattcc cacaatgaaa acacgccgcc ccttgaacca acgggcgttt
      241 tccgtaacac tgaaagaatg taagcgttta cccactaagg tattttcatg gcgaccataa
      301 aggatgtagc ccgactggca ggcgtttcag tcgccaccgt ttcccgcgtc attaataatt
      361 cacccaaagc cagcgaagct tcccggcttg ctgtgcatag tgcaatggag tctcttagct
      421 atcacccgaa cgccaacgcc cgtgcgctgg cgcagcagac cactgaaacg gtcggtctgg
      481 tcgttggtga tgtttccgat ccgtttttcg gtgcaatggt gaaagcggtc gaacaggtgg
      541 cttatcacac tggtaatttt ttattgattg gcaacggtta ccacaacgaa caaaaagagc
      601 gtcaggccat tgagcaactg atccgccatc gctgtgctgc gttggtcgtc catgccaaaa
      661 tgatcccgga tgccgattta gcctcattaa tgaaacaaat gcccggtatg gtgctgatca
      721 accgtatcct gcctggcttt gaaaaccgtt gtattgctct ggacgatcgt tacggtgcct
      781 ggctggctac gcgtcattta attcagcaag gtcatacccg cattggttat ctgtgctcta
      841 accactctat ttctgacgcc gaagatcgtc tgcaagggta ttacgatgcc cttgctgaaa
      901 gtggtattcc ggccaatgac cggctggtga catttggcga accagacgaa agcggcggcg
      961 aacaggcaat gaccgagctt ttgggacgag gcagaaattt cactgcggta gcctgttata
     1021 acgattcaat ggcggcgggc gcgatgggcg tgctcaatga taatggtatt gatgtaccgg
     1081 gtgagatttc gttaattggc tttgatgatg tgctggtgtc acgctatgtg cgcccgcgtc
     1141 tgaccaccgt gcgttaccca atcgtgacga tggcgaccca ggctgccgaa ctggctttgg
     1201 cgctggcgga taatcgccct ctcccggaaa tcactaatgt ctttagtccg acgctggtac
     1261 gtcgccattc agtgtcaact ccgtcgctgg aggcaagtca tcatgcaacc agcgactaac
     1321 cgcagttaaa gcaattccag cgccagtaat tcttcaatgg tctggcgacg gcgaatcaac
     1381 cgcgcctgac cattatcaaa cagaacttct ggtaacagcg gacggctgtt gtagttggat
     1441 gacattgatg cgccatatgc ccctgtatca tgcagtacca gataatcacc tgccttcact
     1501 tccggcaagg cgcgggtttc gacatttccc ccttcctgct gggtaaagac atcgcccgat
     1561 tcacataacg gcccggcgac gacggtttcc accgttggtg cgtgttccag agaacgacca
     1621 tcagctgcca gggcactgat atggtggtaa ctaccgtaca ttgccgggcg catcagatcg
     1681 ttgaacccgg catcaaccag cacaaagtgg cggctaccca tttgtttgac gctccgcact
     1741 tgagtaatta atacgccaga ctgcgctacc agaaagcgac ccggttcaat ttccagtttc
     1801 acagggtggc ccaaatggcg ggcgatttgc tgacgcgcgg cattccacag accataataa
     1861 tgctcggtat caaccgcctc ttcaccctgt tgataaggaa cagaaagccc accgcccgca
     1921 gaaatagcct gtaaatcctg accgaattcg aggacctgac gcaccatagc accacatacc
     1981 tgttccagat gggcataatc gacgccagaa ccaatgtgca tgtgaatgcc gaccagctgt
     2041 agatgatgac gttgtatcac gtccagtgcg gcgggcagat cggtgtacca gataccgtgc
     2101 ttgctgtttt cgccaccggt attggttttt tggctatgcc cgtgaccaaa ccccggatta
     2161 acacgcagcc atacccgatg ccctggcgaa acctgaccca gttggtcgag catatcaaca
     2221 gaacccgcat tcaccggaat ttgcaattca ctgacgcgtt caagcgtcgc ctgatcgata
     2281 acatctgccg taaaaacaat atcatcaggg tgcgtttgcg gattgtaacc cgccgccagt
     2341 gcacgctcta tttcgcctaa cgagacggaa tccactttca cgccctgctc acgcattaag
     2401 cgcaaaatat gaatattgga acaggctttc tgtgcgaagc gcaccacatc aaactgtttc
     2461 agcgctgcaa tctgccgacg aataatttgc gcatcgtaga cccacaccgg gcagccaaat
     2521 tctgcgggca aacgcagcag attttcggcg gtgagatcgg tatcagtgct gaacagtgaa
     2581 tgtggcataa caaactccag ataagtgctt ttttatgatt acgccacatc ataaaaagaa
     2641 taaaaaatat cgatttatgt cgagtctatg caaaaatgat atggattacc ggattgcgag
     2701 agagcgctaa tggccgccgt taacttacgt catattgaaa tttttcatgc ggtaatgacc
     2761 gccggaagcc tgactgaggc ggcacacctg ctacacacct cacagccaac tgtcagccgc
     2821 gaactggcgc gctttgagaa ggtgatcggg ctgaaattgt ttgagcgcgt acgtggacga
     2881 ttgcatccta ccgtgcaagg actgcgtctg tttgaagaag tgcaacgatc ctggtacgga
     2941 ctggatcgca ttgtcagcgc cgcagaaagt ctgcgcgagt ttcgccaggg agaactgtct
     3001 attgcctgcc tgccggtctt ttcgcaatct tttttaccgc agctcctgca accctttctg
     3061 gcacgttatc ccgatgtcag cttaaatatc gtgccccagg aatcaccgct acttgaagag
     3121 tggctctcgg cccagcgtca tgatttagga ctcacggaga cactccatac gcctgcggga
     3181 acagaacgta ccgaattact ctctttagat gaagtgtgtg tgttacctcc gggccatccg
     3241 ctggcggtaa aaaaggtatt aacgccggat gattttcacg gtgaaaacta catcagcctt
     3301 tcccgtactg acagctatcg ccagttgctg gatcaattgt ttactgagaa tcaggttaaa
     3361 cgacgcatga tcgtagaaac ccacagcgcc gcgtcagtct gcgcaatggt acgggcgggg
     3421 gtaggtgttt cggtggttaa cccgctcacc gcactggatt atgcggcaag cggtttagtg
     3481 gtacggcggt tcagtattgc ggttccgttc accgttagcc tgatccgccc cctgcaccgc
     3541 ccgtcatcag cgctggttca ggcgtttagt gagcatttac aagcgggatt accgaaactg
     3601 gtcacttctc ttgacgctat tttgtcgtca gctacgacag cataaaagcg acagcatcct
     3661 cggcatggat cgccgcagta tcaaacacag gcagaacact gcgctcttct ggcaccagta
     3721 aaccaatttc cgtacagcca aaaatgacac cctgtgcgcc ctgttctgca aggcgagcaa
     3781 tcacttgcgc ataataagtg cgtgacgctt cggtaaattg ccccagacac agttcttcaa
     3841 aaataatctg attaattttc gcccgtttat ccgcttcagg aatgagacag ttgatggaaa
     3901 attgttccgt cagccgcccg cgataaaaat cctgttccat ggtgtaacgc gtacccagca
     3961 gcgccacacg agtcattcca gcccggataa ttgcacgtcc agtggcatcc gctatgtgta
     4021 agaaaggcag agagcaacga gactcaatgg catccgccac tttatgcatc gtattggtgc
     4081 ataacacaat accttctgcg cccgcccgct gtaagccaag cgccgcctca gccagaatat
     4141 ccccggtttt gtcccactca ccgcgacgct ggcactcttc tatttcatga aaatcgacgc
     4201 tatgtagcag cacttgcgca gagtgaagcc caccaagccg ctgtttaatg ccttcattta
     4261 tcagacgata gtaaggaata gtggattccc agctcattcc tcccagcaaa ccaattgttt
     4321 tcattcctct ctcctgatgt gtgttaccgc aacaaggctg aataaggaac gagacaaacg
     4381 cctcaacggc caggtgccta atctctatta acgaaaaaag ggccggatgt acagcacatc
     4441 cggcccgtga aatcagacgc cgatatttct caacttttcg cctgccatca gtttgcgctc
     4501 gatatgttcc agcgtgacat ttttggtttc cggaatgagc cagaaagtga tgcccacaaa
     4561 cgcaatgttc agcgcagtgt agagccagaa cgtaccggca gcgccaatgc tatcaagcag
     4621 tgtcaggaag gtcgcgccga taatcatatt cgacacccag tttgtcgtcg ttgagcaggt
     4681 gatgccaaaa tcgcggcatt tcagcggctg aatttcagag cacaagatcc acaccactgg
     4741 cgcggcgctc atcgcataac cggcaataca catcatcgtc atgccaacag agagccagga
     4801 caagccactg gaagccgtac cgttatcaaa ctgcatcagg cagtaaccca gcaccagagt
     4861 gcctaacgcc atcacgctga aaccaatttt cagagccggc ttacgccctg ctttatctac
     4921 cgtaaagacc gcaatgaagg tagcgaacat aaaggtcagc cccacgacca gagtcgcaat
     4981 catctgttgt tctgtggtcg taaagcccgc cattttgaag atacgtggcg cgtagtacat
     5041 gatgatgttc ataccggtaa actgctgcat cgcctgcaac aacataccga gaaacacagc
     5101 acgacggacg ttacggttga tcttaaacag tgcccaaccg ccctgtttta acttcaggct
     5161 ttcacgaatt tcgttgagtt cttctcgcgc tttttccgac gtatcgcgca gcatacgcaa
     5221 cacttcttcc gcctcaatat gacgcccttt ttccgccagc cagcgcgggc tatttggcag
     5281 gaagaccacc agaataatca gcaggactgc tggtaaagca agaaccccca acattgcgcg
     5341 ccagttaccg ctataactga acgctgtatc ggataaaaac gccagcacga tgccgagtgt
     5401 gaccatcaac tggtacatac tgatcatctt accgcgaacg ttttcacttg ccatttcaga
     5461 aagatacaga ggagcggtgt aagacgcgat cccgacagca atgcccagca ccacacgagc
     5521 ggcgattaac atctctacgc tggtcgcaaa tgcggaccct atcgaaccga gcacaaaaag
     5581 gatagccccc gccatcaggc tgtatttacg ccccaggcgg aacgacagcc acccattaaa
     5641 cagcgcacca attgctgcgc cgagcatcat gctactgacc acccattcct gcaaacggct
     5701 ggtcagcaca aagtgatcgg taatgaacgg caacgctccg gcgattacgc cgatatcaag
     5761 accaaataac aatcctgcga ccgcagcagc taccgaaaca aacatattca tacgccgcgt
     5821 atcccgcaaa gaacgtggcg ttaaagcaga ttccgtattg atagtaacca ttttttcctg
     5881 ccagcagaga gtaagacata gtgaaaaaat acgtgaacaa ttcacacagg tgtcaggtcg
     5941 gaaatagcat aaatatggat taaattgctg cgacatgtcg ttatgtgatg gatattccaa
     6001 ttttcaaatt aagttgaatt attgagatta ttattaacca cctaatttta aagcagataa
     6061 aattcataaa gttcattaat tgataattga tatggattat ttcataacca tgatatggat
     6121 tatgatgatc tacaggtata aaaaaccctg ccatgcggca gggtcataaa agtaagagaa
     6181 atgaattaac gagccagcca accgccatcc acggcaatgg tataaccatt cacataatct
     6241 gaagcgctgg aggcaaggaa cactaccggc cccatcaggt cactcggcag tccccaacgg
     6301 ccagctggaa tgcggtcgag aatttccgcg ctacgttgtt catctgcccg tagttgttga
     6361 gtattgttgg ttgccatgta acccggagct atcgcattaa cattaatgtt gtgtttagcc
     6421 cattcattcg ccatcaatcg cgtcacgccc atcacgccgc tttttgatgc ggtataagaa
     6481 ggcacacgga tcccgccctg gaaggagagc attgacgcga tattgataat cttgccgcca
     6541 ttgccttgcg cgataaagtg tttcgccgct gcctgagaca tgaagaatac gctcttgata
     6601 ttcaggttca tgacatcgtc ccagtctttt tcgctgaact cgagagcgtc ttcgcggcga
     6661 atcaatccgg cgttattcac caggatatca atatgaccaa actcagctac cgcgcgatcc
     6721 agcagtgctg gaatgccatc aatctttcgc agatcggcgg tcaggcttaa aaaacgacgc
     6781 cccagcgccg tgacctgctt gatggtttca gtcggttcaa cgatgttaat gccaacaatg
     6841 tcacagcctg cttgcgccag ccccaacgcc atcccctggc ccagcccagt atcacaacca
     6901 gtgacgaccg caactttacc ttcgagagaa aatgcactta aaatcataac aataccttat
     6961 tcgtttatgc ccacaactag cgcaaatctt taacggccac atggtccata tcatcaaaaa
     7021 cctggttttc accgaccatg ccccagataa aggtataagc tttggttccg acgccggaat
     7081 ggatcgacca gctcggggag atcaccgcct gctcgttatg catcacaatg tgacgcgttt
     7141 cttgcggttg ccccatcatg tggaaaacgc aggcgtcatc atccatattg aaatagaaat
     7201 aaacttccat ccggcgctcg tgggtgtgac acggcatggt gttccacaag ttacctggag
     7261 ccagctccgt cagccccata ctcaattggc aggtttccag cacatccggg acaaaatatt
     7321 tgttaatcgt gcgacggtta ctggtgaggt tatcgcctaa cgtgactgga gatacttcgt
     7381 ccggtgtgac ttttttggtg ggatacgtcg tatgcgcggg tgcgcaattg taataaaact
     7441 tcgccggagt ggcggtatca atactggcaa agacaacttc ttttgcacct ttaccaacat
     7501 acagggcttc gcggtgaccg atttcatagc attgaccatc gacagtaatc gtaccggcac
     7561 cgccaatatt gataatacct aactcgcgac gttcaaggaa atagcttacg ccgagttgtt
     7621 taccaacttc cccgccaacg gaaaccgttt tagttaccgg cataatgccg ccaacaataa
     7681 tacggtcaat gtggctgtaa accatggtgt actcatcggc gacaaatacc ttttcaacca
     7741 aaaattcatt gcgcagcccc tgggtatcca gcgtttttgc gtgcgcactg tggatgctct
     7801 gtcttacgtc cacatcaacc tccgataatc agtgaagcgt gaaaatagaa aacgatagtg
     7861 aaacgctgtt tcgttttctc tataagggga tgatatgccc acgggatagg gttttcaatt
     7921 aaaaataaaa caatgtttca tttttaaggt aaggactgaa aaaagtgcag tgtcgatcac
     7981 gaatatgtca tgagaatgga aaaatcaggg agccctgaaa aggtatgtcg actcgccagc
     8041 caccattatt caacagatta atgacggctg atatttatcg gtaaggagga aatgcagagc
     8101 tattcgtcac gttcaatggt caatgccacg ccctggcccc cgccgataca aagcgtagcc
     8161 agtcctttgc gggcattacg tttcaccatt tcatgaacca gagaaacgag gattcggcaa
     8221 ccggaagcgc ctattgggtg accgagtgcg atcgcgccac cattgacatt gacacgacgc
     8281 tcatcccatt caagcatctt gccaaccgaa agcgcctgtg cagcaaacgc ttcattagcc
     8341 tcgataagat cgacatcagc caactgccag cctacacgct ccaggcaacg gcgggtcgca
     8401 tacaccggcg caattcccat caatgccgga tctacaccaa cgctggcaaa tgcgcggatg
     8461 cgggccagca cgggtaaatt caacgctcgt gctttggctt cgctcatcat cattactgca
     8521 gctgcgccat cgtttatgga tgatgcatta cccgctgtca cagaaccgag actatcaaat
     8581 gaaggattta aacgggctaa gccttctgca ctggcgtcag tgcgtggctg ctcatcggta
     8641 tcaacaacca acgtctgacc gttactttgg gtcattaccg ggacgatctc atctttaaat
     8701 cgtccagcgt caatcgccgc tcgcgctttt tgttgcgagc taagtgcgta agcatcctgc
     8761 aactgacggc tgatgccata ttcgcgagcc agattttcgg cggtgacacc aatatgataa
     8821 tcattgaagg catcccacaa cccatcgtgc acaagactat caaccaactg gctattgcca
     8881 agctgtgcac cggtgcggct atcagtcaga acatgtggtg cgcggctcat gttttcctgg
     8941 ccaccggcga tgacaatatc agcctcgcca cactgtatcg cctgagtagc cagatgcagt
     9001 gctttaagcc cggaaccgca aacgtcatta atagtgattg cagaaacgct attaggaaga
     9061 ccacctttaa tagccgattg ccttgccgga ttctgccctg cccctgcagt caacacctga
     9121 ccaagaatta cttcatccac cgcatatgca ggaacgccgg tacgttctat taacgctttc
     9181 acgaccaggc tgccaagttc cacggcggaa tgacccgcta acgcaccacg gaagcagccg
     9241 atgggtgtcc gtaacgcccc gacaatcaca acgtctttca tcacaacctc gcaccaatta
     9301 acagtgcaat agtaaatgaa tgttaattac agttatctta attgtttaaa aaaagtgatt
     9361 tttatcacaa aggaaatatg cctgagtagc aatcagagac ataactggca cgtaaggttt
     9421 gcaaccacta acccaccaat agagaggtag aaagggcgtt aatctcccat acttaacctg
     9481 gtttatggta aattgccctc cattttgttt aatttgtaga tgatacgttc agataatgtc
     9541 taatatttgg tcaaaagaag aaactctgtg gagtttcgcg ctctacggca cagccgttgg
     9601 tgcaggcacg ctcttccttc ctattcagtt aggttcggcg ggggctgtgg tcctgtttat
     9661 tactgctctg gtcgcctggc ctttaacata ttggccacat aaagccttat gccagttcat
     9721 cctctcatcg aaaacatcag caggtgaagg gataacgggc gcggtaacac actactatgg
     9781 caagaagatt ggtaatctga ttaccacgct gtacttcatc gccttttttg tcgtcgtgtt
     9841 gatatatgca gtggcaatta ccaactcact tacagaacag ctggcaaagc atatggttat
     9901 tgatcttcgc atccgtatgt tggtgagtct gggggttgta ttaatcctga atctcatttt
     9961 tctgatggga cgccatgcca ctattcgggt aatgggattt ttggtattcc cattgattgc
    10021 ctatttctta tttctttcta tttacctggt cggtagttgg caacctgatc tattaacaac
    10081 ccaggtagag ttcaatcaga atacccttca ccagatatgg atatcgattc ccgtgatggt
    10141 tttcgctttt agccatacgc ccattatttc tacgtttgcc atagacagac gtgaaaaata
    10201 tggcgaacac gctatggata aatgcaaaaa aattatgaaa gtcgcttatc tcatcatctg
    10261 cataagtgta ttgttctttg tctttagctg cctgctttct attccacctt cgtatattga
    10321 agcggctaaa gaagaagggg ttaccatttt atcggcgctt tctatgctgc cgaacgcccc
    10381 agcatggttg tcaatttccg ggattattgt cgcagtagtt gcgatgtcga aatcattcct
    10441 gggtacgtac tttggcgtta ttgaaggtgc cacagaggtc gtcaaaacaa cattacagca
    10501 ggttggtgta aagaaaagtc gtgcatttaa ccgcgcacta tcaattatgt tggtatcgct
    10561 gattaccttc attgtttgtt gcattaaccc gaacgcgatt tcgatgattt acgcgatcag
    10621 cggcccgctc attgccatga tacttttcat catgcctacg ctgtcaacgt atctcatccc
    10681 ggcgcttaaa ccctggcgtt ccatcggaaa tctgattacc ctgatcgtgg gtatcctgtg
    10741 cgtatcggta atgttcttta gc
//
LOCUS       DAZGQL010000142        10707 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.142, whole genome shotgun sequence.
ACCESSION   DAZGQL010000142 DAZGQL010000000
VERSION     DAZGQL010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10707
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.142"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..2113)
                     /locus_tag="ACHKIR_004606"
     CDS             complement(<1..2113)
                     /locus_tag="ACHKIR_004606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992251.1"
                     /translation="MAFSDEQRPEAQLGNQNRSSLNIKQPGENYWQDFFSNPQNAIDH
                     STSFSLGDVLPTMGKGVAQSVRGTGEMARGLGDAMIQSPVKTGARILNEFSRMGLPGV
                     ATVQDIFAGGSRGADEVIDTLPDGKNVVTDTVGKGLKATGKAVSDGAKASDEWLTEQM
                     SPGAVRALNTPITEGYDDSAVLLAKGVNLIGALVPDMVAGGVARKVGDVTLRKMLTAG
                     LEKKYIAAGMQPERATALAAEAVDKKMPDLFQAGLITHSTASAQGQSAMAAADAVLNA
                     DYSELAQSPKFQQTFLSIDADPQHAQLTDRQKMDLAKERVADEVRAQLATDPELLAVN
                     AMAAKLGDAQLFNLVTRGTAKTVKSGIVRNATEQGVINGAQNAYSRYQENMALRETAG
                     MDVSPWEGVTDAGIEGTVFGAAMGAPFGAVAGYRGRRQAAEEAAMRDAETVQQDDAAP
                     QPEPVDPVAQHRESMQGMNREQLLEQYADADMATEGDASAAHRREAASQLLNELDEQA
                     KRQAVMNELKAKPRSELLEEYRRLSQKEERTETEEQQFQAIREVIRPQQEVTPEAQSQ
                     PENAEDGNGSIYPTVRFRDPNEVRIEINGNGASRPAERIEKVRPDNRYFTDEKSAMGS
                     DVFRNAAATGLKPSVVKKGENQYAVEMDNPAFSEDVATETINTLADGERIADADPMEQ
                     PAFMRDPRFRGFTGDDTEVQAR"
     gene            complement(2199..3461)
                     /locus_tag="ACHKIR_004607"
     CDS             complement(2199..3461)
                     /locus_tag="ACHKIR_004607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992252.1"
                     /translation="MSGFAQGLLAGFSTVDQAMTRRKELGLREAQLAQQQKNNERDFE
                     FAQSQFEHNKDVDQRNFDYRAKVDDRNYTLQEREFNANQNYRNASLGMEQQRLQLQKY
                     NQRRLEYNDMLARDQPVMAALGKAIDAGDHDAASHLFGKLSDANPLKMMSTVGYAAKA
                     GQAVNNLQKIFDDKPDRAIASLNTPENLDVLSGVFAPELQQRIGMPDSTGEKTIKEAR
                     IGSIVPAQQEGYVLIGLDLTYSDGSTAHKPVTEYGSAHPDDQTVLAIPVDKAIAQVRD
                     RSKFAEISKNYGYFMPKQQGLSLAKLQEVASNVAADAIKNGRDAQAEVDEFYAANGLQ
                     PHQQKVQQRKTQQLLQKWQTWAAGDPVKQEFVKEAAANAPALLEPGREKEVEIYYQNH
                     LRDKKAEHEKALDYSASAAAEKIKGWMK"
     gene            complement(3472..3723)
                     /locus_tag="ACHKIR_004608"
     CDS             complement(3472..3723)
                     /locus_tag="ACHKIR_004608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HFO9992253.1"
                     /translation="MGYGLLDIANQSRREALQGISDADRRRTEIEASNKQLAAQQKAQ
                     KKQNIGTGIGTGAAIGASVGGPVGAVAGAVIGGIAGSLF"
     gene            complement(3733..4179)
                     /locus_tag="ACHKIR_004609"
     CDS             complement(3733..4179)
                     /locus_tag="ACHKIR_004609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992254.1"
                     /translation="MEYGKYETLARYGYTGAARPQGDWQTSAALTRQQYDDWRTRYLP
                     RVARLADLGENNSLMNAQLARVGGLATSSLRTAQMAQDNQMARYGVSRPDNPDSNTLG
                     LRNALAIAGAKNGIREAEQDRQMNILTGASAPARQKLSVGGQLVAV"
     gene            complement(4182..4838)
                     /locus_tag="ACHKIR_004610"
     CDS             complement(4182..4838)
                     /locus_tag="ACHKIR_004610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992255.1"
                     /translation="MGGSKGGGDTKVKPTAAQIAQEEVAWKGWQDYQKILRPAEDNFM
                     EKVDDLNSEQQYDNIAGTTNLGYQKQFGEARKELAGNLAQSGVDPSSGRFNAVMNANQ
                     SDQVTGQIDTTTRGQVSQADKYVAGLQDVAALGSGQKADALQSFNSLADSSLAKAKSD
                     AQAAFTKQQGRASLVGAGLGAAGAYAMHKAGGSGGSGGAKTPGTGANAIQHQAQNWRL
                     "
     gene            complement(4932..5333)
                     /locus_tag="ACHKIR_004611"
     CDS             complement(4932..5333)
                     /locus_tag="ACHKIR_004611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992256.1"
                     /translation="MAPVSGERMNDKILRYMQRVVRNSRNPEFMNEVKDACLKKQAFC
                     FEAPDGFLVLRSVLSDDGIPYVLVLLGVCTGSNSVERYLPEVKTLTRLAGGRWAEFHT
                     ARRGFIRLGKRLGFERMPDDEDGFMVFRIAV"
     gene            complement(5390..5530)
                     /locus_tag="ACHKIR_004612"
     CDS             complement(5390..5530)
                     /locus_tag="ACHKIR_004612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO9992257.1"
                     /translation="MSQKSLITVTICMTVIFTIWMLHGSLCEFRLNLWGAEFAAFLQC
                     KQ"
     gene            complement(5763..6497)
                     /locus_tag="ACHKIR_004613"
     CDS             complement(5763..6497)
                     /locus_tag="ACHKIR_004613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO9992258.1"
                     /translation="MKSIATLVVCAISGIACVNLSAHAAEGEHTISLGYAHFQFPGLK
                     DFVKDATAHNRETFSHFINRNYFSSLGEYTDGRVSGYEGKDKNPQGINIRYRYEITDD
                     FGVITSFTWTRSLTNSQTFIDVQSADHTRKIKNPAASARTDIRANYWSLLAGPSWRVN
                     QYMSLYAMAGIGVAKVTADLKIKDNINSSGGFSESNSTKKTSLAWAAGAQFNLNESVT
                     LDVAYEGSGSGDWRTSGVTAGIGLKF"
     gene            complement(6588..7205)
                     /locus_tag="ACHKIR_004614"
     CDS             complement(6588..7205)
                     /locus_tag="ACHKIR_004614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992259.1"
                     /translation="MYGLSIMKPDGSVWISPGFTPQCLINKGTIPATEKSFFKTSIPS
                     GKSCFFFIRTEKKADVMYTHEQIDGYHALRLHVIVRGTNPGVTTVYAFANMVTPPSEY
                     GIAMYNPDGEMIYHGEMMLLDAKLIPVDIKFEKDLGYPCAIMPALVGYYNWKRTPYDR
                     PIYTTSTGATGNKIYSCEHYSGGATWDIRKPYIDKVLVINTSVYD"
     gene            complement(7211..7489)
                     /locus_tag="ACHKIR_004615"
     CDS             complement(7211..7489)
                     /locus_tag="ACHKIR_004615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992260.1"
                     /translation="MEYGFAIYNRNNVNVTGVLTPVFFLDRFTAESGSKTYTNKPDGK
                     SLQAVCCLFPWNNVFADRKVPKITINGNTVTWSNLEQGMGSYIYTFWG"
     gene            complement(7504..8772)
                     /locus_tag="ACHKIR_004616"
     CDS             complement(7504..8772)
                     /locus_tag="ACHKIR_004616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFO9992261.1"
                     /translation="MTRKPWRAGKDLSTVVENMEIGTGQRGDGRHAFVTREELVGLKL
                     ARRRTSGGASYALNPGIEIDSTLMTVDFPTKPLNFKATGGFGSVLLEWDMPNYRGHSL
                     TEIWRGTEDDLADAVLVATTPGQVYGDPVDPGWSGFYWIRFVNAAGVKGPWNAEKGTQ
                     AQTQIGVKAIIDQIRDEAAKSPVVSELRKEIKNAQGQAVKDAAIKTTEVVGTLREETT
                     RTIGGIETRISTLDSSTSESLNEVDKRITKLDKEGGEAFLAMWSKKAGVDGITAGIGI
                     VAGKDSEGRPVSQVAISASQLFVFDPNNPDNTAYPFAVSGGKVVIPKAMIYDAVIETL
                     VSRKVVADEVKAGVSITSPVIRSAVIQNGNFQVDSQGNLNIGGLFSVTSQGQLTIRYS
                     NQNVGLVIRNDKIEVYDQNGRLAVRIGRLR"
     gene            complement(8769..10394)
                     /locus_tag="ACHKIR_004617"
     CDS             complement(8769..10394)
                     /locus_tag="ACHKIR_004617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992262.1"
                     /translation="MPYIDITTMRGMMPGVIASMLPDHSAVLAENCHFRYGVITPEHQ
                     MSEAEKTFAIKPKTIFHYRDDFWFAWTDVVDVIRSPVAQDAHGRIYYTDGRFPKVTDA
                     TIATKGDGNHPTSSYRLGIPAPTTAPVCTVQQGGDVSDDNPNDDETRFYTETFVSDYG
                     EEGPPGPASLEVTLRTPGTAVQLTLSPVPLQNASIKRRRIYRSASGGGEADFLLVAEL
                     DASVLSYTDKIPGKNLGPSLATWDYLPPPENMTGLCLMANGIAAGFAGNEVMFSEAYL
                     PYAWPEVNRHTTAEDIVAICPLGTSLVVATKGEPYLFSGVSPSTISGSRIPSMQACLS
                     RRSMVAMEGFVLYAGTNGLVSVDVNGNTALATEKIISPEQWQSQFNPASIVAYSWRGE
                     YIACYTKPDGKQDVFVFSPVNMDIRYLSTPFDCAWVDLAKDMMRVVTGDKMSVLAGGS
                     LPSTIRWHSKIFSLPERTSFSCIRVKSPAPERVGITIMADDVPVIHFAPGTFKGSVVR
                     LPAATGQNWQVMVSGFGQVERITLSTSMSEMPV"
BASE COUNT         2515 a         3091 c         2310 g         2791 t
ORIGIN      
        1 ggcgggcctg tacctccgta tcatcccccg tgaaaccacg gaatcgcggg tcacgcatga
       61 acgcgggctg ctccatcggg tcagcatcag caatacgctc tccgtcagcc agggtgttaa
      121 tggtttccgt tgccacatct tcagagaacg caggattatc catttcaacg gcatactgat
      181 tctcgccttt cttcactacg gacggtttca ggccggtagc ggcggcatta cggaaaacat
      241 cactccccat ggcgcttttc tcatccgtga aataacggtt gtccgggcgc accttttcaa
      301 tgcgttccgc tggtctggac gcaccattcc cgttaatttc aatgcggact tcattcgggt
      361 cccggaaccg caccgtcggg taaatgctcc cgttaccatc ctccgcattt tcaggctgtg
      421 actgtgcttc cggcgtcact tcctgttgtg ggcgaatgac ttctcgtatt gcctgaaact
      481 gttgttcttc agtctcggtg cgctcctctt tctggctgag tctgcggtat tcctcaagca
      541 gttcagaacg cggcttcgcc ttcagctcat tcatcacagc ctgtcgcttc gcctgttcgt
      601 ccagttcatt caacaactgg ctggcagctt cccggcgatg agctgcggat gcgtcaccct
      661 ctgttgccat atccgcatca gcatactgtt ccagaagctg ctcgcgattc atgccctgca
      721 tggattcacg atgctgtgcc accggatcaa ccggttcggg ttgcggggct gcgtcgtcct
      781 gctgcacggt ttcagcatca cgcatggctg cttcttctgc ggcctgacgt ctgccacgat
      841 atccggcaac cgcaccgaat ggagccccca tcgcagcacc gaaaacagtg ccttcaatcc
      901 ctgcgtcagt caccccctcc catggcgata catccattcc ggcggtctca cgcaatgcca
      961 tgttttcctg atagcgcgaa taggcgttct gtgccccgtt aattacgccc tgttccgtgg
     1021 catttctgac aatgccgctt ttaacggtct tcgctgtgcc tcgtgtcacc agattaaaca
     1081 gttgtgcgtc acccagtttt gccgccatgg cattcacagc cagcaattca ggatcggttg
     1141 ccagctgcgc gcgcacctca tcggcaacac gctcttttgc cagatccatt ttctggcgat
     1201 cagtaagctg tgcatgctgc gggtcggcgt caatggacaa aaacgtctgc tgaaatttcg
     1261 gtgactgcgc cagctcagag taatccgcat taagaacagc atctgctgcc gccattgcac
     1321 tctgcccctg tgcactggct gtggaatggg tgatcaggcc cgcctggaat aaatccggca
     1381 tttttttatc gacagcttct gctgccagtg ccgtggctct ttccggctgc atccctgccg
     1441 cgatgtattt tttctccagc ccggcggtca gcatttttcg cagtgtgaca tcacccacct
     1501 ttctagccac accgccagca accatatcag gtacaagcgc accaatcagg tttacaccct
     1561 tcgccagcaa gaccgcagaa tcatcatagc cttctgttat cggcgtattc agcgcacgaa
     1621 cagcacctgg cgacatctgt tctgtcagcc attcatccga ggctttggca ccatcactga
     1681 cagccttacc ggttgctttc agacctttac cgacagtatc agtgaccacg ttttttccat
     1741 caggcagggt atcgatgacc tcatcagccc ccctgctgcc accggcaaaa atatcctgaa
     1801 cagttgcgac acccggtagc cccatacggc tgaactcatt taaaatacgc gcccctgttt
     1861 ttaccgggct ctgaatcatc gcatcaccga gtccacgggc catttcccct gttccccgga
     1921 cggactgggc aacaccttta cccattgttg gcaatacatc accgaggctg aacgatgtgc
     1981 tgtgatcaat ggcattctgc gggttgctga aaaaatcctg ccagtaattt tctcccggct
     2041 gcttaatgtt caggctgctg cggttctggt tacccaattg cgcttcaggg cgctgctcat
     2101 ctgaaaaagc catacagact ccagtaaaaa aaagcccgac acgatggtcg ggcgtaagtg
     2161 aacgatatta acggggagcg cgttactgcc gcatatcttt acttcatcca gcctttgatc
     2221 ttctcggcag ctgcggaagc gctataatcc agcgcctttt catgctctgc ctttttatcc
     2281 cggaggtgat tctggtaata aatttccacc tccttttcac gaccaggttc cagcaaagca
     2341 ggcgcattgg cggcagcctc ttttacaaac tcctgtttaa ccggatcgcc cgccgcccac
     2401 gtctgccact tctgtaaaag ctgttgcgtt ttccgttgct gaactttctg ttgatgcggc
     2461 tgtaaaccat tcgcagcata aaattcatcc acctcagcct gagcatcacg gccattcttg
     2521 atcgcatccg ccgctacgtt gctggctact tcctgaagtt tcgccagaga aagtccctgc
     2581 tgcttcggca taaaataacc ataatttttc gatatctctg caaatttgct gcgatccctg
     2641 acctgagcga tagccttatc aacgggtatc gccagcacgg tttgatcatc agggtgcgca
     2701 ctgccgtatt ctgttacagg tttatgcgcg gtggagccat cgctgtatgt gagatcaagg
     2761 ccaataagta cgtacccttc ctgctgcgcc ggtacgatac tgccaatcct ggcctctttt
     2821 atcgttttct ccccggttga atcaggcatg ccaatacgct gttgtagttc cggggcaaac
     2881 acgccggaaa gcacatcgag attttccggg gtattcagcg aagcaatcgc cctgtccggc
     2941 ttatcatcaa agattttctg caggttgttc acggcctgac ccgctttcgc agcatagcct
     3001 accgttgaca tcatcttaag cggattggcg tccgataatt taccgaacag atgagaagcc
     3061 gcatcatggt cacccgcatc aatcgctttt cccagcgccg ccatcacagg ctgatcgcgc
     3121 gccagcatat cgttatactc aagccgtcgc tggttgtatt tctgcaactg gagtcgttgc
     3181 tgttccatcc ccagcgacgc attccggtaa ttctggttgg cgttaaactc cctttcctgc
     3241 agtgtgtaat tgcggtcatc aactttggct ctgtaatcaa agttccgctg atcaacgtct
     3301 ttattatgtt caaactgaga ctgcgcaaat tcaaaatcgc gctcgttatt tttctgttgc
     3361 tgagcaagct gtgcttctcg caaaccaagc tccttacgac gggtcattgc ctggtcaaca
     3421 gtgctgaatc cggcaagtaa cccctgtgca aatccgctca ttcaccactc cttaaaacaa
     3481 agaaccagca atgccgccaa ttactgcacc agcaacagcg ccaacaggac caccaacgga
     3541 tgcgccaata gccgcccccg taccaatgcc cgtaccgata ttctgttttt tctgagcttt
     3601 ctgttgagca gccagttgtt tatttgatgc ctcaatctct gtgcgtcgtc tgtctgcgtc
     3661 acttattccc tgtaatgcct cacgccgcga ctgatttgca atatccagta aaccgtatcc
     3721 catattgccc ccttacactg ccaccagttg tccgccaaca ctcagtttct gtcttgccgg
     3781 tgcagaagcc cccgtcagaa tattcatctg gcgatcctgt tcggcttcac ggataccatt
     3841 tttcgcgcca gcaattgcca gggcattacg taaccccagc gtattactgt cgggattatc
     3901 cgggcggctt accccgtatc tcgccatctg gttatcctgc gccatctgcg ctgtacggag
     3961 actggaagtg gcaaggccgc ccacccgggc aagctgtgca ttcatcaggc tgttgttctc
     4021 gccaaggtca gccagccttg ccacgcgggg caaatatctg gttcgccagt cgtcgtattg
     4081 ttggcgtgtc agcgctgctg acgtctgcca gtcaccctgt ggacgggcag ctccggtata
     4141 accgtatctc gcaagtgttt cgtatttacc gtactccata atcacagtct ccagttctga
     4201 gcctgatgct gaatggcatt agcgccggtg ccaggtgttt tagcaccgcc acttcctccg
     4261 ctaccgccag ccttatgcat cgcatatgca cctgccgcac ccaaaccagc gccaacaaga
     4321 gaggctcgcc cctgctgttt cgtaaacgca gcctgtgcgt ccgatttagc ttttgccaga
     4381 ctgctgtctg ccagcgagtt aaaactctgt aacgcatccg ccttctgacc agaaccgaga
     4441 gctgcaacat cctgtagccc ggcaacatac ttatctgcct gcgatacctg cccccgtgtg
     4501 gttgtgtcaa tctgcccggt tacctgatca ctctggttcg cattcattac cgcgttaaaa
     4561 cgaccactgg acgggtcaac accggactga gcaagattac ccgccagctc cttccgtgct
     4621 tcgccaaact gtttctgata ccccagattt gtggtgccag caatattgtc gtactgctgc
     4681 tcactgttca gatcatcgac cttttccatg aagttatctt cagccgggcg gaggattttt
     4741 tgataatcct gccacccttt ccaggccact tcttcctgtg ctatttgcgc tgctgttggt
     4801 tttactttgg tatcaccacc gcctttactt ccacccataa tggccccctg gataacaaaa
     4861 aaccctgccg gagcagggtc agaatgtgaa ttacaatgtt ggtttaaaca caatgatgaa
     4921 aactgtagca gtcagaccgc tatcctgaac accatgaagc catcctcatc atccggcatt
     4981 cgctcaaagc ccagacgttt tcccagccgg ataaatcccc gccttgccgt atgaaattca
     5041 gcccagcgtc cgccagccag acgggttaat gtcttcacct ccggcagata acgctcaacg
     5101 ctgttactcc ccgtacacac gcccagcaac accagaacat aagggatacc atcgtcactg
     5161 agcacagaac gcagcaccag aaagccatca ggagcctcaa aacaaaacgc ctgcttttta
     5221 aggcaggcgt ctttaacttc attcataaat tcagggttgc gggaatttct caccacacgc
     5281 tgcatatacc ggagaatttt atcgttcatt ctctcacctg aaacgggtgc catatcggct
     5341 gaacctcagc aaccagttga cggggacttt cgtccccgcc gcggttttcc tactgcttac
     5401 actgtaagaa cgccgcaaac tccgctcccc acaaattcag ccggaactca cacaacgagc
     5461 cgtgtaacat ccagatggtg aagataaccg tcatacaaat tgtgacggtg ataagcgatt
     5521 tttgcgacat agcgcttgac tccttttaca gagaggcgct aaccttctac ttgcttaagg
     5581 tacgaagtgt tagggcctcg ggttaacgtt aagttgactc ggggcctttc cacatcaggc
     5641 cttcaggttc accctccagc catcagccga aaggcaccca cgcataattt acgatttttg
     5701 ccctgtacgg gcaataaaaa acccgccatc acagcgggta gtaaatggtt accggataca
     5761 ggtcagaatt tcaggccaat gccagcagta acgccactcg tgcgccagtc gccagagccg
     5821 gaaccttcgt aagccacatc cagtgtaaca ctctcattca ggttaaactg tgcacctgca
     5881 gcccacgcaa gggaggtttt tttcgtgctg ttgctttcag aaaatccgcc actactgtta
     5941 atattgtcct taattttcag gtcagcggta actttagcca cgcctatccc tgccatcgca
     6001 tataaactca tgtactgatt aacccgccat gaaggccccg ctaacagact ccagtaattc
     6061 gccctgatat ccgttctggc agaagctgcc ggattcttaa tcttcctggt atgatcggct
     6121 gactgcacat caataaatgt ctgtgagtta gtgagagaac gcgtccatgt aaaagaggtg
     6181 ataacgccaa aatcatccgt tatctcgtag cgatacctga tattaatgcc ctgtggattt
     6241 ttatccttgc cttcatatcc actgacccga ccatctgtat attcgcccaa tgaagaaaag
     6301 tagtttctgt tgatgaaatg actgaaagtc tccctgttat gagcagtcgc atcctttaca
     6361 aaatccttca gtcccggaaa ctgaaagtgc gcatacccca gagaaattgt atgctctcct
     6421 tctgctgcat gtgcagataa atttacacag gcaatcccgg agattgcaca cacaaccagt
     6481 gttgctatac tcttcattta agatatcctg tattcagata acacgctaaa ctttttaata
     6541 atacatcttt ttagacattt taaatgaata ttaaagactc gcttcaacta atcatatact
     6601 gatgtattaa taaccaggac cttatctata tacggctttc gaatatccca tgttgcacca
     6661 ccggaataat gctcacagga atatatttta tttcctgtag caccagtgga tgtggtataa
     6721 atcggtcgat cataaggagt tcttttccag ttataatacc cgaccagtgc aggcatgatt
     6781 gcgcatggat atccaaggtc cttttcaaat ttgatatcaa caggtattaa cttcgcgtca
     6841 agcagcatca tttcgccatg ataaatcatc tcaccgtccg ggttatacat ggcgatacca
     6901 tactcagaag gtggagtaac catattcgcg aaagcataaa ccgtcgtaac accagggttc
     6961 gttcccctga caattacatg aagccttagt gcatgatatc catcaatctg ttcatgcgtg
     7021 tacatgacat cggccttctt ctctgttctg ataaagaaaa aacaactttt gcctgacggg
     7081 attgatgttt taaaaaaaga cttttcagtc gccggtatgg tgcctttgtt gatcagacac
     7141 tgcggcgtaa aacctggact tatccatacg ctgccatccg gcttcataat gctcaaacca
     7201 tacatgacac ttatccccag aatgtataaa tataagatcc cataccctgc tcaagattcg
     7261 accacgtcac cgtattgcca ttaatggtta tcttcggtac tttccgatcc gcaaatacat
     7321 tattccaggg aaataaacaa catacagcct gcaatgattt cccgtcgggt ttattcgtgt
     7381 acgtctttga gccagactcc gctgtaaatc tgtccaggaa aaataccgga gtcagcacgc
     7441 ccgtaacatt aacgttattt ctgttataaa tggcaaaacc gtattccaat actcacctcc
     7501 tgatcagcgt aatctgccta tgcgaacagc cagtcgtcca ttctgatcat aaacctcaat
     7561 tttatcattg cggatcacca gtcctacatt ctgattagag taacgaattg tcagttgccc
     7621 ttgtgacgta acactgaaaa ggcctccaat attcaggtta ccctgagaat caacctgaaa
     7681 gtttccgttc tgaataacgg cactccggat aactggcgaa gtgatactta ccccggcttt
     7741 tacctcatcc gccacaacct tccgcgacac cagtgtttca atcaccgcgt cataaatcat
     7801 cgctttcggg atcacaacct tgccacctga taccgcaaac ggataggcgg tgttatccgg
     7861 gttgttcggg tcaaagacaa acaactgcga cgcagaaatt gcaacctgac ttacaggcct
     7921 gccttcactg tcttttccgg cgacaatccc gatccccgca gtgataccat caactcccgc
     7981 tttttttgac cacattgcca gaaacgcctc accgccttct ttatccagtt tagtgatgcg
     8041 cttgtcgacc tcattaagcg attcactggt tgacgaatcc agtgtgctaa tgcgggtttc
     8101 aataccacca atcgttcttg tcgtttcttc cctgagagtc cccacaactt cggttgtctt
     8161 aattgcagca tcctttacag cctgcccctg cgcgtttttt atttctttac gcagctcgga
     8221 cacaaccggc gactttgcag cctcatcgcg gatctggtca atgatggcct tcacgccgat
     8281 ctgtgtttgt gcctgagtgc ctttttcagc attccatgga cctttcactc ctgccgcgtt
     8341 aacaaaacgt atccagtaaa atcccgacca gccagggtca accggatcgc cgtaaacctg
     8401 ccccggcgtc gtggcaacca gcactgcatc agcaaggtca tcctccgtac cccgccagat
     8461 ttcagtcagt gaatgtccgc gataattagg catatcccat tcaagaagaa ccgagccaaa
     8521 tcctcctgtc gccttaaaat tcagcggttt tgtgggaaaa tcaacagtca ttaaagtact
     8581 gtcaatctca atacccggat tcagtgcata tgaggcacca cccgatgttc gacgccgggc
     8641 gagtttaaga ccaaccagtt cctcacgggt cacaaatgcg tggcgtccgt caccacgctg
     8701 cccggtgcca atttccatgt tctccacaac tgtggataaa tccttccccg cacgccacgg
     8761 ttttctggtc ataccggcat ctccgacatc gatgtactca gggttattcg ttccacctgc
     8821 ccgaatccgg ataccatcac ctgccagttt tgcccggttg ctgccggaag tctcaccaca
     8881 cttcccttaa acgtacccgg cgcaaaatga atcacaggaa catcatcagc cataatggtg
     8941 atccccaccc gctcaggcgc cggagatttc accctgatac aggaaaaaga ggttctttca
     9001 ggtaatgaaa aaatttttga atgccacctt atcgtggagg gcagagagcc cccggcaagc
     9061 actgacattt tgtctcctgt caccacacgc atcatatctt tcgcgagatc aacccatgcg
     9121 cagtcaaacg gtgtactgag ataacggata tccatgttca ccggactgaa tacaaacaca
     9181 tcctgcttac catccggttt cgtgtaacag gcaatgtatt caccacgcca ggaataagcc
     9241 acaatggacg ccgggttaaa ctgactctgc cactgttcag gtgaaataat cttttccgtt
     9301 gccagtgctg tattaccgtt tacatcaaca gataccagac cgtttgtccc ggcatagagt
     9361 acgaatccct ccatcgccac catacttctt cggctcaggc atgcctgcat ggaaggaatt
     9421 ctggagccag aaattgtgga aggcgataca ccactgaaca gataaggctc cccctttgtc
     9481 gccaccacca gtgacgttcc cagcggacag atagccacaa tatcttctgc cgtcgtgtga
     9541 cgattcactt ccggccatgc atacggcaga tacgcttccg aaaacatcac ttcattaccg
     9601 gcaaacccgg cggcaatacc attagccatc aggcaaaggc ctgtcatatt ctctggcggc
     9661 ggcaggtaat cccatgtagc cagagaaggt ccaaggtttt tccccggtat tttgtccgtg
     9721 taactgagca cggatgcatc cagttcagcc acaagtaaaa aatccgcttc tcctccacct
     9781 gatgcagagc gataaatccg gcggcgttta atactggcat tctgcaatgg caccggagac
     9841 agcgtcagct gtaccgcagt ccccggagta cggagtgtta cctccagaga cgccggacct
     9901 ggcggacctt cttcaccata atctgagaca aaggtttccg tataaaaccg ggtttcgtca
     9961 tcattcgggt tatcgtcaga aacatcaccg ccctgctgaa cagtacagac aggtgctgtt
    10021 gtcggcgcgg gaatccccag acgatacgat gatgtcgggt gattcccgtc cccttttgtg
    10081 gcaatggtcg catccgtcac tttaggaaaa cgcccgtcag tgtagtaaat acgcccgtgg
    10141 gcgtcctgag cgaccggact gcggatcaca tccaccacat ccgtccatgc aaaccagaaa
    10201 tcgtcacggt aatgaaaaat ggttttcggc ttaatcgcaa atgttttctc agcctctgac
    10261 atctggtgtt caggcgtgat cactccatag cgaaaatgac agttttctgc cagtacagca
    10321 gaatgatctg gcagcataga tgcaataacg cctggcatca tcccgcgcat agttgttata
    10381 tcgatataag gcatggttgt ctggtatccg aaaatttgaa tggcaggtga gtcactgaat
    10441 tgaagtttta aaggcggcat acaattgcgc ctgtgcagaa tacagatttc cagaaatctg
    10501 aaaaattact cttatcagag aagagcgtca gaaacacgat cggcatcact gtttctgcaa
    10561 acttccctgt ggcagtcttt attcgccata taacatagca ctgaatgtct tcattgattt
    10621 ttccattgta gttactcctt ccttgtaccg ggggttggtc atgcccccgt tttttattac
    10681 ccctgttccc ttaattgtac tgccagg
//
LOCUS       DAZGQL010000143        10641 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.143, whole genome shotgun sequence.
ACCESSION   DAZGQL010000143 DAZGQL010000000
VERSION     DAZGQL010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10641
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.143"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            88..1455
                     /gene="dcuC"
                     /locus_tag="ACHKIR_004618"
     CDS             88..1455
                     /gene="dcuC"
                     /locus_tag="ACHKIR_004618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFO9992263.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGIFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASRAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCIIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKALDNVPPLYYAILPVMPLILMLGSLFLAHVGLMQSELHLVVV
                     MLLSLTVTLFVEFFRKHNLRETMDDVQAFFDGMGTQFANVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAIGAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIAT
                     ITLFY"
     gene            complement(1499..1996)
                     /gene="sspB"
                     /locus_tag="ACHKIR_004619"
     CDS             complement(1499..1996)
                     /gene="sspB"
                     /locus_tag="ACHKIR_004619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HFO9992264.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDASIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(2002..2640)
                     /gene="sspA"
                     /locus_tag="ACHKIR_004620"
     CDS             complement(2002..2640)
                     /gene="sspA"
                     /locus_tag="ACHKIR_004620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein SspA"
                     /protein_id="HFO9992265.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(3035..3427)
                     /gene="rpsI"
                     /locus_tag="ACHKIR_004621"
     CDS             complement(3035..3427)
                     /gene="rpsI"
                     /locus_tag="ACHKIR_004621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFO9992266.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(3451..3879)
                     /gene="rplM"
                     /locus_tag="ACHKIR_004622"
     CDS             complement(3451..3879)
                     /gene="rplM"
                     /locus_tag="ACHKIR_004622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFO9992267.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(4098..5225)
                     /gene="zapE"
                     /locus_tag="ACHKIR_004623"
     CDS             complement(4098..5225)
                     /gene="zapE"
                     /locus_tag="ACHKIR_004623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HFO9992268.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSTP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGAKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYDIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            5419..5817
                     /gene="zapG"
                     /locus_tag="ACHKIR_004624"
     CDS             5419..5817
                     /gene="zapG"
                     /locus_tag="ACHKIR_004624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Z-ring associated protein ZapG"
                     /protein_id="HFO9992269.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            5971..7338
                     /gene="degQ"
                     /locus_tag="ACHKIR_004625"
     CDS             5971..7338
                     /gene="degQ"
                     /locus_tag="ACHKIR_004625"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HFO9992270.1"
                     /translation="MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            7428..8495
                     /gene="degS"
                     /locus_tag="ACHKIR_004626"
     CDS             7428..8495
                     /gene="degS"
                     /locus_tag="ACHKIR_004626"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HFO9992271.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINPRRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGM
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVNNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(8558..9496)
                     /gene="mdh"
                     /locus_tag="ACHKIR_004627"
     CDS             complement(8558..9496)
                     /gene="mdh"
                     /locus_tag="ACHKIR_004627"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030060 - L-malate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFO9992272.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
     gene            9931..10401
                     /gene="argR"
                     /locus_tag="ACHKIR_004628"
     CDS             9931..10401
                     /gene="argR"
                     /locus_tag="ACHKIR_004628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HFO9992273.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
BASE COUNT         2622 a         2687 c         2646 g         2686 t
ORIGIN      
        1 taaatagcct tgcaaaaaat ataacatcat tgttttcaat ctgccgttta tgggattgac
       61 cattttcttt tgacacggag ttcaacaatg ttcggcataa ttatatctgt catcgtatta
      121 attacgatgg gctatttgat cctgaaaaac tacaaacctc aggtggtgct ggctgccgca
      181 ggtatcttcc tgatgatgtg cggtgtctgg ttagggttcg gtggtgtgct cgatcccgcc
      241 aaaagcagcg gctacttgat cgtcgatatt tataatgaaa tcctgcgcat gctgtccaac
      301 cgcattgctg gattggggct gtcgattatg gcagtgggcg gttatgcccg ctatatggag
      361 cgcatagggg caagtcgcgc gatggtgagc ttgttaagcc gcccgttaaa actcattcgc
      421 tcgccgtata ttattctgtc ggcaacttac gtcatcggcc aaatcatggc gcagtttatt
      481 accagcgcct ccggtctggg tatgttgctg atggtcacct tatttccgac gctggtgagt
      541 ctgggagtaa gtcgtctctc tgcggtggcg gttatcgcaa ccaccatgtc cattgagtgg
      601 gggattctgg aaacgaactc catttttgct gcccaggtcg caggaatgaa aattgccact
      661 tacttcttcc actaccagct tccggtcgcc tcttgcatca ttatctcggt ggcgatctcc
      721 cactttttcg tgcaacgcgc tttcgacaaa aaagataaaa atatcaatca cgaacaggca
      781 gagcaaaaag ctctcgataa tgtcccgccg ctctattacg ccattttacc ggtgatgccg
      841 ttaatcctga tgctcggctc gctgttcctt gcccacgtcg ggctgatgca gtcagaactg
      901 catctggtgg tggtgatgtt actgagtttg acggtgacgc tgtttgttga gttcttccgc
      961 aagcataact tgcgcgaaac aatggacgat gtgcaggcgt tttttgacgg catgggtacg
     1021 cagtttgcca acgtggtaac gctggtggtc gcgggtgaaa tatttgcgaa aggcttaacg
     1081 acgattggca ctgtcgatgc ggttatcagg ggggcggagc attctggtct gggcggtatt
     1141 ggcgtgatga ttattatggc gctggtcatt gccattggtg ccattgtgat gggctcaggc
     1201 aatgcgccgt ttatgtcatt tgccagtctt attccgaata tcgcagccgg actacatgta
     1261 ccagcggttg taatgattat gccgatgcat tttgccacga cgctagcgcg cgctgtttcg
     1321 ccgattactg cggtggtggt cgttacgtca ggaattgcag gcgtttcgcc ttttgcggta
     1381 gtgaagcgga cagcgatccc catggcagtc ggtttcgtgg tgaatatgat tgccactatc
     1441 acgctatttt attaagcgat taaaaagaca aaacaggccg ccagggcctg ttttcatttt
     1501 acttcacaac gcgtaatgcc ggtcgaccac cgcgtggtgg ctgcggaggt tcatcgtcag
     1561 gatgagtgtc atcatcgtga tctggcttgt cgccatcaat aaccgacata acggtttcgt
     1621 tgtctgccga tgcctcttca tcattcatga tgctggcatc ttcatcgtag gcagcttcag
     1681 gttcaaacat cgtgcctgcg ccattttcac gggcgtagat agccagcacg gcagccagcg
     1741 gcacagaaac ctgacgcgga atgccaccaa agcgcgcgtt aaagcgcacc tcatcattcg
     1801 ccagttccag attgccgaca gctcgcggcg caatgttgag tacgatttgc ccgtcacgcg
     1861 catattccat aggaacctgc acgccaggga gcgtcacatc caccaccagg tgcggcgtga
     1921 gctggttatc cagcaaccac tcatagaatg cacgcagcag atagggacga cgtggtgtta
     1981 gctgtgacaa atccatacag attaactccg gcccagacgc atttcacgtt cagcttcagt
     2041 taaagaagca aggaaagagt cacgctcaaa gacgcgggtc atatagcctt tcagctcttt
     2101 cgcacccggg ccgctgaact cgatgcccag ttgcggcaga cgccacagca gaggagcaag
     2161 gtagcaatcg accaggctga actcatcgct caggaagtac ggcttctgac cgaagaccgg
     2221 cgcaatcgcc agcagttctt cgcgcagttg cttacgtgcg gcatcggctt cagaagctga
     2281 accgttgatg atggtgttca tcagcgtgta ccagtctttt tcgatgcgat gcatgtacag
     2341 acggctttca ccgcgagcta ccgggtaaac aggcatcagt ggcggatgcg ggaaacgctc
     2401 atccagatat tccataatga tgcgagattc ccacagggtc agctcacgat ccaccagggt
     2461 cggaacgctc tgattcgggt tgaggtcaat cagatcctga ggcggattgt ccttttccac
     2521 gtgttcgatc tcgaaactta cacctttctc agccagcaca atgcggacct gatggctata
     2581 gatgtcagta ggaccggaaa acagcgtcat taccgaacgt ttgttggcag cgacagccat
     2641 gaaaacctcc aggtatagtc agaattttta ctgctaccag ccaccaggtg gccagtcaga
     2701 agttgtgtta cccagtaagg aacgactctc tttgttcgaa aatcaaacaa aaaatgagca
     2761 atacccgaca tttgggcaga aaattggatg atagtttacc agattttgcg acctttgtgg
     2821 tgagtcgatg ccggaaatgg ggaaaaagag atgcgcttta gtctgaaata gttgacttag
     2881 tcccttattg gcgatgtggt ttttgtttta cctgtctgtc aggtggcagc aaaaagcaac
     2941 ttcccatttt ttacgctgat gcagatttta gccataaaaa aacccgccga agcgggtttt
     3001 ttcgcaaatt gttttctgcc ggagcagaag ccaattaacg tttggagaac tgcggacgac
     3061 gacgtgcttt acgcagaccg actttcttac gttcaacctg acgagcgtca cgagtaacga
     3121 agccagcttt acgcagttca gaacgcaggg actcgtcgta ttccatcaga gcgcgggtga
     3181 taccgtgacg gatcgcacca gcctgaccag agataccacc acctttaacg gtgatgtaca
     3241 ggtccagttt ctcaaccatg tcgaccagtt ccagcggctg acgaactacc atgcgagcag
     3301 tttcacgacc aaagtactgt tccagagaac gttggttgat tacgatttta ccgttgcccg
     3361 gtttgatgaa aacgcgagct gcggaacttt tgcggcgacc agtgccgtag tattgatttt
     3421 cagccattgc ctataatccc gattagatga ttagatgtca agaacttgcg gttgctgtgc
     3481 cgcgtggttg tgctcgttac ccgcgtaaac tttcagttta cggaacatag cacgacccag
     3541 cgggcctttt ggcaacatgc ctttaaccgc gatttcaatc acacgctcag gacggcgagc
     3601 aatcatctct tcaaaggtcg cttgtttgat accaccgatg tggccggtgt ggtgatagta
     3661 cactttgtca gtacgcttgt tgccggttac agcaactttg tcagcgttca gaacgatgat
     3721 gtaatcaccg gtatctacgt gcggagtgta ttccgctttg tgcttaccgc gcaggcgacg
     3781 agccagttca gtagccagac ggcccagagt tttaccggtc gcgtcaacaa cataccagtc
     3841 gcgttttacg gtttctggtt tagctgtaaa agttttcatt aaaagcttac ccaataaaaa
     3901 gttacacgtt ggtgaacacc caaacgtctt caattgttga ggttcacacg acaaagtccg
     3961 gcaaacctac cccttcgaat agcctatgcc agcacacaaa aagttttggg aaaaaaactt
     4021 tcttgtaacg tggggtcgca ggattataga gaagtcgggg tcaaagatcg accccttttt
     4081 gtgatttgtg gcaggtttta acccgccaaa tgctcgcgct tcagatactc ttcgctttgc
     4141 atctcttgca gacgtgacag gcaacgctgg aactcaaatt tcagccgctc gccctgataa
     4201 atgtcatata gcggcacttc tgcactcacc actaatttga catggcgctc gtaaaactca
     4261 tccaccagcg caataaaacg tcgcgcttcg ctctccatca accgcgtcat aactggtaca
     4321 tcaaacaaca tgactgtgtg aaagaggcgt gagagcgcaa tatagtcatg ctgactgcgg
     4381 gcgtcgacgc acagcgtagt aaaagagacc gccagcgtct ggttctcgac gcccattgtc
     4441 gccagcggcc gatggttgat ttctaatgtc ggtgaatttt ctcgtttcgc ccccgccagc
     4501 gccaaccata gtttatccat ttgcgcccgg gtttcatcgt tgaggggcga aagccacaga
     4561 tgcgcctgag tgagtgtacg cagtcgataa tcaacaccag cgtccacgtt cattacatca
     4621 caatgctgtt taatggcatc gattgcaggc aaaaaacgcg cacgttgtag gccatttcga
     4681 taaagttcgt ccggcggaat atttgacgtc gctaccaggg taataccgcg ggcgaacagg
     4741 gctttcatca gaccgccaag tagcatggca tcggtaatat cagaaacaaa aaattcgtca
     4801 aaacagagca cgtcagtttc tgctttaaag cgatcggcaa taatttccag cggatcggtc
     4861 tgcccctgca aagcagttag ctcttcatgt acccgcagca taaaacggtg aaagtgcaga
     4921 cgctgtttcc gctctcccgg caggctttga tagaaaaggt ccatcagcca ggttttcccg
     4981 cgtcctacac cgccccacat atataagcca cgcactggcg tatgctttgt gtcttcgcgt
     5041 ttaccccaca gcttaccgac ccgcgccatt agcccactcg tcctgggggc tggaggcgtg
     5101 ctattgatga gttcctgata aataatttcc aggcggctga cggcctcttt ttgaacgtcg
     5161 tcgggttgat ggctgccttc attgagcgcc ttcaggtatt gcgatgttgg ggtaacgctt
     5221 tgcatgatct tattgttatt ccttgaataa tcggtgcgcc gttgttcacg gttgacgaaa
     5281 aaaaggccgt tctacactac gcgatatgca gtcgggattc cacttctgtg gaattaacgg
     5341 ttatagtggc ataatcagcc gcaggcatgg agcctgaagc caacacccta cggaaacaaa
     5401 agacaacggg agatgttcat gacctgggaa tatgcgctaa ttgggttagt cgtcggcatc
     5461 attattggtg ctgtggccat gcgttttggt aatcgtaaac tacgccagca acaggcgttg
     5521 cagtacgaac tggaaaagaa taaagctgaa ctggacgagt atcgcgaaga gctggttagc
     5581 cactttgccc gcagcgcgga attactggat accatggcgc acgactatcg ccagctgtat
     5641 caacacatgg cgaaaagctc cagcagcctg ctgccggaac tgtctgctga agcaaacccg
     5701 ttccgtaatc gtctggcaga gtctgaagcc agcaacgatc aggcaccggt gcaaatgcct
     5761 cgcgactatt ctgaaggcgc atccggccta ctgcgtactg gcgcgaagcg cgactaattt
     5821 atttttcggg cgcagccatt gcgccctcct cttctctccc tccccgacta tcatttaatc
     5881 tggtgtctca ttgttagccg tctgaaaatt caataacatc aaactgtttt gaatctcttt
     5941 tcttatcatt caggtacgag agtaggaata atgaaaaaac aaacccagct gttgagtgca
     6001 ttagcgttaa gtgtcgggtt aactctctcg gcgtcatttc aggccgttgc gtcgattcca
     6061 ggccaggttg ccgatcaggc ccctctcccc agtctggccc caatgctgga aaaagtgctt
     6121 ccggcagtgg tgagcgtacg ggtggaagga acggccagtc agggacagaa aatcccggaa
     6181 gaattcaaaa agttttttgg tgatgattta ccggatcaac cggcacaacc cttcgaaggt
     6241 ttaggctccg gtgtcatcat caacgccagt aaaggctatg tgctgactaa caaccatgtg
     6301 attaatcagg cacagaaaat cagtattcag ctcaatgatg ggcgcgagtt tgatgcaaaa
     6361 ctgattggta gcgatgacca gagcgatatc gccctgttgc aaattcaaaa cccgagcaaa
     6421 ttaacgcaaa tcgctattgc cgactccgat aaattgcgcg tcggtgattt tgccgtggcg
     6481 gtcggtaacc catttggcct tgggcaaacc gccacctctg gcattgtttc cgcattaggc
     6541 cgcagtgggt tgaatcttga aggtctggaa aactttatcc agacagatgc ttccattaac
     6601 cgcggtaact ccggcggtgc actgttaaat cttaacggtg agttaattgg catcaacacg
     6661 gcaatccttg cgcctggtgg cgggagcgtc gggattggat ttgccatccc cagtaatatg
     6721 gcgcgaacac tggcgcagca gcttatcgac tttggtgaaa tcaaacgcgg tctgttaggc
     6781 atcaaaggca cagagatgag tgccgatatc gccaaagcct tcaaccttga cgtgcagcgt
     6841 ggcgcgtttg tcagcgaagt gttgccaggt tctggctcgg caaaagcggg cgtcaaagcg
     6901 ggcgatatta ttaccagcct caacggcaaa ccgctgaata gctttgctga gttgcgctct
     6961 cgtatcgcaa ccaccgagcc gggcacgaaa gtgaagcttg gcctgctgcg taacggcaaa
     7021 ccactggaag tagaagtgac gctcgatacc agcacctctt cgtcggccag cgctgaaatg
     7081 atcacgccag cgcttgaagg tgcaacgttg agcgatggtc agctaaaaga tggcggcaaa
     7141 ggtattaaga tcgatgaggt tgtcaaagga agcccagctg ctcaggcagg cttgcaaaaa
     7201 gacgatgtga tcattggcgt caaccgcgat cgggtgaact cgattgctga aatgcgtaaa
     7261 gtgctggcgg caaaaccggc catcatcgcc ctgcaaattg tacgcggcaa tgaaagcatc
     7321 tatctgctga tgcgttaatg tcgtaaaccg ggcatcaggc tgacgtgtga tgtccggtta
     7381 actcgtggta tgctgctgcc gttccctttt ttaatgacgc ctccatcatg tttgtgaagc
     7441 tcttacgttc cgttgcgatt ggattaattg tcggcgctat tctgctggtt gccatgcctt
     7501 cgctgcgcag ccttaacccg ctttccactc cgcaatttga cagtaccgat gagacgcctg
     7561 ccagctataa tctggcggtt cgccgcgccg cgccagcggt ggttaacgtt tacaaccgtg
     7621 gtttgaacac caactctcac aaccagcttg agatccgcac cctgggatcc ggtgtaatca
     7681 tggatcaacg cggttatatc atcaccaata aacacgtcat caacgacgcc gatcagatca
     7741 tcgtcgcctt acaggatgga cgtgtttttg aagcgttact ggttggctct gactctctaa
     7801 ccgatctggc ggtgctgaaa attaacgcca caggcggttt accaacgatt ccgatcaatc
     7861 cacgccgcgt accgcacatt ggcgatgtgg ttctggcgat cggtaacccg tacaacctcg
     7921 ggcagaccat tacccagggg attatcagtg ccacgggtcg aatcggtctg aacccgaccg
     7981 ggcggcaaaa cttcctgcaa accgatgctt ccattaacca cggtaactct ggcggcgcgc
     8041 tggtgaactc gctgggcgaa ctgatgggca ttaacacgct gtcgtttgat aagagtaacg
     8101 atggcgaaac gccggaaggt atcggctttg cgattccttt ccagttagcg accaaaatta
     8161 tggataaact gatccgtgat ggccgtgtga tccgtggcta cattggcatt ggtggacgtg
     8221 agatcgcccc attacatgcg cagagtggcg gcatggatca actgcaaggg atcgtggtta
     8281 acgaagtgtc gcctgatggc ccagcggcaa atgcaggcat ccaggtcaac gatctgatta
     8341 tctctgtgaa taacaaaccc gctatttccg ccctggagac aatggatcag gtcgcggaaa
     8401 ttcgccctgg ttcggtgatc ccggttgtcg tgatgcgtga cgataaacag ttaacattgc
     8461 aggtcaccat tcaggaatat ccggcgacca actaaggtca gcgtaaaaaa acaaaaaccg
     8521 gagtctgtgc tccggttttt tattatccgc taatcaatta cttattaacg aactcttcgc
     8581 ccagggcgat atctttcttc agcgtatcca gcataccttc cagcgcgttc tgttcaaatg
     8641 cgctcagggt accgatagat ttacgctctt ccacgccgtt tttacccagc agcagcggtt
     8701 gagagaagaa acgtgcgtac tgaccgtcgc cttcaacgta ggcacattcg acaacgcctt
     8761 gttcgccctg cagtgcgcga accagagaca gaccaaaacg tgcagctgcc tggcccatag
     8821 acagggttgc agacccgcca ccggctttcg cttcaaccac ctcagtaccc gcgttctgga
     8881 tacgtttggt cagatcagcc acttcctgct cggtaaaact aacgccagga acctgtgaca
     8941 gcagcggcag aatggtaaca ccagagtgac cgccaataac cggcacttca acttcgcctg
     9001 gctgtttgcc tttcagttcc gcaacaaagg tgttggaacg aatgatatcc agcgtggtaa
     9061 cgccgaacag tttgtttttg tcataaacac cggctttttt cagcacttca gcagcaatcg
     9121 caactgtggt gttaaccggg ttagtgataa taccaatgca cgctttcggg caggttttcg
     9181 caacttgctg taccaggttt ttcacgatgc cggcgttaac gttaaacagg tcggaacgat
     9241 ccatacccgg tttacgcgct acacctgcag agataagaac gacatctgcg ccttccagcg
     9301 ccggagtcgc atcttcacca gaaaaacctt tgattttcac agcagtaggg atatggctca
     9361 gatcgacagc cacaccggga gtcactggag cgatatcata cagagagagt tctgaacctg
     9421 aaggcagttg ggtttttaac agtagtgcaa gcgcctggcc aataccgcca gcagcgccga
     9481 ggactgcgac tttcatccta aactccttat tatattgata aactaagata tgttgctccg
     9541 ctgccgcgac cttaatccac aaaattgccg tttacaatga ccacatctca agaatgtgta
     9601 gtcacgcaag tttagcgttt atgcatttaa ttgccgtaat caggaaccta acttacgtaa
     9661 ttaacagtcg cttatgcatt agcgcaacat tccaacaggt ggtgacaata taccctaccg
     9721 ttcagccaaa acaacatcaa tttgataaca attaatttac ttttaagcag aatttgcatg
     9781 ccgtgacgca ggcatgtttc tcaataacga aatttgataa aatcccgctc tttcataaca
     9841 ttatttcagc cttcttcagg gctgactgtt tgcataaaaa ttcatctgta tgcacaataa
     9901 tgttgtatca accaccatat cgggtgactt atgcgaagct cggctaagca agaagaacta
     9961 gttaaagcat ttaaagcatt acttaaagaa gagaaattta gctcccaggg cgaaatcgtc
    10021 gccgcattgc aggagcaagg ctttgacaat attaatcagt ctaaagtctc gcggatgttg
    10081 accaagtttg gtgctgtacg tacacgcaat gccaaaatgg aaatggttta ctgcctgcca
    10141 gctgaactgg gtgtaccaac cacctccagt ccattgaaga atctggtgct ggatatcgac
    10201 tacaacgatg cagttgtcgt gattcatacc agccctggcg cggcgcagtt aattgctcgc
    10261 ctgctggact cactgggcaa agcagaaggt attctgggca ccatcgctgg cgatgacacc
    10321 atctttacta cccctgctaa cggtttcacc gtcaaagacc tgtacgaagc gattttagag
    10381 ctgttcgacc aggagcttta atctctgccc cgtcgcttct ggcggcgggg aaaatgttgc
    10441 ttttccctct caccccccct gctttctcct gcgattaatt taacgaatag tgcgttttat
    10501 cgcgacatat cattcacata atgaatacat gggttaaaaa aagcacatta tgcaaaattc
    10561 attatctaat tgaaaagaat agaattaata ataaataatc gtgtttttaa ttcttttttg
    10621 ttattaaaac tcacattttt a
//
LOCUS       DAZGQL010000144        10542 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.144, whole genome shotgun sequence.
ACCESSION   DAZGQL010000144 DAZGQL010000000
VERSION     DAZGQL010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10542
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.144"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(319..1455)
                     /locus_tag="ACHKIR_004629"
     CDS             complement(319..1455)
                     /locus_tag="ACHKIR_004629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFO9992274.1"
                     /translation="MDLLIVLTYVALAWAVFKIFRIPVNQWTLATAALGGVFLVSGLI
                     LLMNYNHPYTFTAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQAR
                     VDRLQADLMTATHNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFS
                     ERDIDDARQNFLAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLE
                     QTVIRAPSNGYVTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGD
                     DAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPND
                     DIDALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(1458..1820)
                     /locus_tag="ACHKIR_004630"
     CDS             complement(1458..1820)
                     /locus_tag="ACHKIR_004630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFO9992275.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            2357..4270
                     /gene="mtlA"
                     /locus_tag="ACHKIR_004631"
     CDS             2357..4270
                     /gene="mtlA"
                     /locus_tag="ACHKIR_004631"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HFO9992276.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGASPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            4635..5783
                     /gene="mtlD"
                     /locus_tag="ACHKIR_004632"
     CDS             4635..5783
                     /gene="mtlD"
                     /locus_tag="ACHKIR_004632"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFO9992277.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIRK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYGLPHKNLIEGIAAAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            5783..6370
                     /gene="mtlR"
                     /locus_tag="ACHKIR_004633"
     CDS             5783..6370
                     /gene="mtlR"
                     /locus_tag="ACHKIR_004633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HFO9992278.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(6381..6590)
                     /gene="yibT"
                     /locus_tag="ACHKIR_004634"
     CDS             complement(6381..6590)
                     /gene="yibT"
                     /locus_tag="ACHKIR_004634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibT"
                     /protein_id="HFO9992279.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRQLYRPKQVEEK"
     gene            6874..7236
                     /locus_tag="ACHKIR_004635"
     CDS             6874..7236
                     /locus_tag="ACHKIR_004635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HFO9992280.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
     gene            7795..8478
                     /locus_tag="ACHKIR_004636"
     CDS             7795..8478
                     /locus_tag="ACHKIR_004636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFO9992281.1"
                     /translation="MNLKKTLLSVLIIVPLCLLAGCDYIEKASKVDDLVTQQELQKSK
                     IEALEKQQELDKRKIEHFEKQQTTIINSTKTLAGVVKAVKDKQDEFVFTEFNPAQTQY
                     FILNNGSVGLAGRVLAIDAVENGSVIRISLVNLLSVPVSNIGFHATWGNERPTDAKAL
                     AKWQQLLFNTTLNSTLQLMPGQWQDINLTLKGVSPNNLKYLKLSINMANLQFDTVQSA
                     ETRQRKNKK"
     gene            8522..>10542
                     /locus_tag="ACHKIR_004637"
     CDS             8522..>10542
                     /locus_tag="ACHKIR_004637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ESPR-type extended signal peptide-containing
                     protein"
                     /protein_id="HFO9992282.1"
                     /translation="MNKIFKVIWNPATGSYSVASETAKSRGKKSGRSKLLISVLVASG
                     LLSSFGALANNYDGQPTDYGDGSAGDGWVAIGKGAKANTFMNTSGASTALGYDAIAEG
                     EYSSAIGSKTLATGGASMAFGVSARAMGDRSVALGASSVANGDRSMAFGRYAKTNGYT
                     SLAIGDSSLADGEKTIALGNTAKAYEIMSIALGDHANASKEYSMALGASSAASAANAI
                     AVGRNSAAAGVDSLAFGRLSAANAANAIAMGAESKAAENATAVGTNAEANGLNSIALG
                     SGSIADVDNTIALGNQSQAVAAGAIAIGQGNKADGANAIALGNGSITGGVNAIALGQG
                     SYAGLENGTAIGAQASAQGKNSVALGAGSVATDADTVSVGNTTAQRQIVNMAAGDIST
                     TSTDAINGSQLYAISKSVADNLGGGATVNAQGVVTSPNYRLKSGIFGTVGDALTGLDN
                     NTLQWDSLKKAYSAAHGTDTTSTITNVKDGAISDTSKDAVNGSQLKTTNDNVATNTAN
                     ITTNTNSINTLTDSVGDLKDDALLWNGTAFSAAHGTEATSKITNVKDGDLTAGSTDAV
                     NGSQLKTTNDAVAANTTNIATNTTNIATNTTNITNLTDAVDSLGDDSLLWNDAAKAFS
                     AAHGTDATSKITNVKDGDLTAGSTDAVNGSQLKTTNDAVAANTTNIATNT"
BASE COUNT         2824 a         2581 c         2592 g         2545 t
ORIGIN      
        1 tataacttaa atatttttaa atgtgactgt catccttcag ataataaagc catcatcgca
       61 atagatggaa aagcactcct gctctttttt gattagagcc gtggcagagg aacaactcat
      121 atcatcattg cgatctcgac aatgtatagt ctgatccttc ggcagatcaa gacggataaa
      181 aaatccaatg agttcacact catcccgaac ttcttaacat aatggatatt aacgaaaaat
      241 catcacagcc gatgaaataa tatgcaaaaa taattagtcc ttacaaggag caaagttcca
      301 taatctcccc cctccccctc aatgatccaa ataaagataa tgcatccaac tggtcattct
      361 cagcagcact ttccgcatca ccgaaacatg gctgaaatgg tcggagtaaa ctgccacttg
      421 ggcgtagatg ccgtcgggta aggcatcgat atcatcgtta gggtccagtt caatggttcc
      481 cagcacaccg tccgtgccag gcacgaccgt taatgattgc aataccccct gcgcctgata
      541 agaaccgcct ggcacgacag gtaaaatact agtcagtttg ccgtgaaaca cctgcccagg
      601 tagcgcgtta aacaccactt ccgcatcatc gccgggtttc agacgtaaca gcgagttttg
      661 ccgaaattgg gcgacaattt gccgtttttg ctcggggatg aaaaccatca ccggacgcaa
      721 cggcaaggca gctgcgtatg tacctgggcg gatcagtacc tgagtaacgt agccattgct
      781 tggtgcgcga atgacagtct gctcaaggtt atattttgct tcagtaagtt gcgctcttaa
      841 gctcacaatc tgcgattgct cgccgttaac catactgtcg agctggctct ggatctgcgc
      901 ctgctccgcc accgagcctt tcaccagcgc gtcctgcgcg aggaaatttt gccgcgcatc
      961 gtcgatgtca cgttccgaga acggattcac cgccgcctgg ctgcctttca ggtaacgttg
     1021 ataattttta aacagacggt cgcgctccgc tgaaacctgg gtggtgttgg cctgcgcttc
     1081 agtgagctgc gcgcgcagcg tctttatatt atgcgtcgcc gtcatcaggt cagcctgaag
     1141 tctgtcaact cgcgcctggt aacgaaccgg gtcgagctta aaaagcacct cgcccttttg
     1201 aataagctga ttatttttgt cggtgacttc agtaacaatt cccgtcacct gtggcgtgat
     1261 ggggatcgct atcactgcct tttgcgcggt aaaagtgtaa gggtggttgt agttcatcaa
     1321 caaaatcaaa ccactcacca gaaacacgcc tcccagcgcc gcggtcgcca gcgtccactg
     1381 attcaccgga atgcggaaga ttttaaagac cgcccacgcc agcgccacgt aagttaaaac
     1441 aatcaataga tccatattta gatctccggg aacgtggaat agtcagtctt tttctcggcg
     1501 gcgagttgat gctccagccg ggaaattcgg tcagaaagtg cggcgatttc cgggtcagtc
     1561 gctttctcct gcgacgcaac atgtgactgc ataccccagc cacgctccgg ttgatagagc
     1621 gtcgcccaga tccacaggaa cggccagata acatgcagag taaacaggct cacccagccc
     1681 gccgtatgaa tagcgtcggc atggggatga ttgcgctttt tggcaatcag atatgggatg
     1741 tcatgtatcg cgatgattcc ataaaaaatc accagaaaga cgaagatcag cactcccaac
     1801 gcgaaatagt ttagaaacat atctgcctcg gattcacgtt tatcagtgtt gttttttggc
     1861 tggcagccag aagggagtca ggctgatatt ttgacaataa tccgggttcg cgattctcgc
     1921 cataacacca aagaataatt tttagaggtg atgagttgct tagttacata acgattttat
     1981 gacgaaggca taacatgctg tagatcacat caggtgaacg ccgtaagaaa atatcttgtg
     2041 attcagatca caaagatcca acaaaccacc aaaacaaaaa tgtgacatta gtcacattta
     2101 aatgccattt ttagcgaaaa tcgccgtctt gttgcttttt tacacaagca ttttgtgatg
     2161 agcatcacgt caattacctc tctaccccct atatttatgt gacccatatc acacaaaagg
     2221 ccgtcgactg gacagttaac cgattcagtg ccagatttcg cagtatctac aaggtccggc
     2281 tacctctgcc gccacattaa caaaaaacct cgggcttcca gcctgcgcga cagcaaacat
     2341 aagaaggggt gtttttatgt catccgatat taagatcaaa gtgcaaagct ttggtcgttt
     2401 cctcagcaac atggtgatgc caaatatcgg cgcgtttatc gcgtggggta tcatcaccgc
     2461 gttatttatt ccaacagggt ggttaccgaa cgagacgctg gcgaagctgg tcgggccgat
     2521 gatcacttat ctcctgccgc tgctgatcgg ttataccggt ggtaagctgg taggcggcga
     2581 acgtggcggc gtagtcggtg ccatcaccac catgggcgtt atcgtcggcg cagacatgcc
     2641 gatgttcctc ggttctatga ttgcaggtcc gctgggcggc tggtgcatta agcacttcga
     2701 ccgctgggta gacggtaaga tcaaatccgg ttttgagatg ctggtgaata acttctccgc
     2761 aggcatcatc gggatgatcc tcgctattct ggcattcctc ggcattggcc cgattgttga
     2821 agccctgtcc aaaatgctgg ctgcgggcgt taacttcatg gttgtccatg acatgctgcc
     2881 gctggcgtct atctttgttg aaccggcgaa aatcctgttc ctcaacaacg ccattaacca
     2941 cggtatcttc tcgccgctgg gcattcagca gtcccatgaa ttgggtaaat ccatcttctt
     3001 cctgattgaa gctaacccag gtccaggtat gggcgtgctg ctggcgtaca tgttctttgg
     3061 tcgtggcagt gctaaacaat ctgcgggcgg tgcggcaatc atccacttcc tgggtggtat
     3121 ccacgaaatt tacttcccgt atgtgttgat gaatccgcgt ctgatcctgg ccgttatcct
     3181 cggcggtatg actggcgtgt tcacgctgac tatcctgggc ggtggtctgg tttctccggc
     3241 atctccgggt tctatccttg ctgtactggc gatgacacca aaaggtgctt atttcgctaa
     3301 catcgcaggt gtgtgtgcgg cgatggctgt ctccttcgtt gtctctgcca ttctgctgaa
     3361 aaccagcaaa gtgaaagaag aagatgatat tgaagcagca actcgtcgta tgcaggacat
     3421 gaaagctgag tctaaaggtg cgtctccgct gtctgcgggc gatgtgacta acgacctgag
     3481 ccacgtacgt aaaatcatcg ttgcctgtga cgccggtatg ggttccagtg cgatgggcgc
     3541 aggtgtgctg cgtaagaaaa ttcaggatgc aggtctgtcg cagatttcgg tcactaacag
     3601 cgcgatcaac aacctgccgc cagatgtgga cctggtcatc actcaccgtg acctgaccga
     3661 acgcgctatg cgccaggttc cgcaggcaca gcatatttcg ctgaccaact tcctcgacag
     3721 cggcttgtac accagcctga ccgaacgtct ggttgctgcc cagcgccata ctgaaaacga
     3781 agtaaaagtg aaagacagtc tgaaagacag ctttgacgat tccagtgcta acctgttcaa
     3841 gctaggcgcg gagaacatct tcctcggtcg caaagcggcc accaaagaag aagcgattcg
     3901 ttttgctggc gagcagctgg tgaaaggcgg ttacgttgag ccagaatacg ttcaggcgat
     3961 gctggatcgt gaaaaactga ccccgactta tctgggtgag tctatcgcgg tgccacacgg
     4021 tacggttgaa gcaaaagatc gcgtactgaa aacgggcgtg gtgttctgcc agtacccgga
     4081 aggcgtgcgc ttcggtgaag aagaagatga cattgcccgt ctggtgattg gtattgctgc
     4141 ccgtaacaac gagcacattc aggttatcac cagcctgacc aatgcactgg atgatgaatc
     4201 cgtcatcgag cgtctggcac acaccaccag cgtggatgaa gtgctggaac tgctggcagg
     4261 tcgtaagtaa tacaatctca ccctctccac atggagaagg tggggcttat tgcctgatgc
     4321 gctacgctta tcaggcctac aaaacaaagc acagtttgtt gaatttgcac gttcttgtag
     4381 gccggataag gcgtttacgc cgcatccggc gctgcccctc tccacatgga gaagatgggc
     4441 taattgcctg atgcgctacg cttatcaggc ctacaaaaac aaagcacaat ttgttgaatt
     4501 tgcacgttct tgtaggccgg ataaggcgtt tacgccgcat ccggcgcggc ccctctccac
     4561 atggagaggg tttgggtgag ggaaaagcct caccccagcc ctctcgggta aaaacattga
     4621 tgaaggttaa tactatgaaa gcattacatt ttggcgcagg taatatcggt cgtggcttta
     4681 tcggtaaact gctggcagac gcgggtatcc aactgacgtt tgccgatgtc aatcaggtgg
     4741 tacttgatgc cctgaatgcc cgtcatagct atcaggttca tgtggtcggt gaaaccgagc
     4801 aggtagatac cgtttccggc gtcaatgccg tcagcagcat tggtgatgat gtcgttgatc
     4861 tgattgctca ggttgattta gtcactaccg ccgttggccc ggttgtgctg gaacgtattg
     4921 ctccggcaat cgccaaaggg ctggtgaaac gtaaagaaca aggtaatgaa tccccgctga
     4981 acatcatcgc ttgtgaaaac atggtccgcg gcaccacaca gctgaaaggc catgtgatga
     5041 acgccctgcc agaagatgcc aaagcgtggg tagaagaaca cgttggcttt gtcgattccg
     5101 ccgttgaccg catcgtgccg ccttcggctt ctgcaaccaa cgatccgctg gaagtgacgg
     5161 tagaaacatt cagcgaatgg attgtcgata aaacgcagtt caaaggcgcg ctgccaaaca
     5221 tccctggcat ggaattaacc gacaatctga tggcatttgt cgaacgtaaa ctcttcaccc
     5281 tgaatacagg tcatgctata accgcgtacc tcggaaaact ggccggtcat cagaccattc
     5341 gtgatgcgat tctcgacgag aaaatccgcg cggtggtaaa aggtgcgatg gaagaaagtg
     5401 gtgcagtatt gatcaagcgc tacggctttg acgcagacaa acatgcggcg tacatccgga
     5461 aaatcctcgg tcgtttcgag aacccgtacc tgaaagatga tgtagagcgc gtaggccgtc
     5521 agccgctgcg taaactgagt gctggcgacc gtctaatcaa gccactgctc ggaacgctgg
     5581 aatatggtct gccacataaa aacctgattg aaggtattgc cgctgcaatg cacttccgca
     5641 gtgaagatga tccgcaggct caggaactgg cagcactgat cgctgacaaa ggtccgcagg
     5701 cggcgctggc acagatttcc ggtcttgatg ccaacagcga ggttgtatcc gaggcggtaa
     5761 ccgcttataa agcaatgcaa taatggtgga ccaggcgcag gacaccctgc gcccgaataa
     5821 cagattgtca gatatgcagg caacaatgga acaaacccag gcctttgaaa accgtgtgct
     5881 tgagcgtctg aatgctggca aaaccgtgcg aagctttctg atcaccgccg tcgagctttt
     5941 gaccgaggcg gtaaatcttc tggtgcttca ggtattccgc aaagacgatt acgcggtgaa
     6001 gtatgctgta gaaccgttac tcgacggcga cggtccgctg ggcgatcttt ctgtgcgttt
     6061 aaaactcatt tacgggttgg gcgtcattaa ccgccaggaa tacgaagatg cggaactgct
     6121 gatggcattg cgtgaagagc taaatcacga cggcaacgag tacgcgttta ccgacgacga
     6181 aatccttgga ccctttggtg aactgcattg cgtggcggcg ttaccaccgc cgccacagtt
     6241 tgaacctgca gactccagtt tgtatgcaat gcaaattcag cgctatcaac aggctgtgcg
     6301 atcaacaatg gtcctttcac tgacagagct gatttccaaa atcagcttaa aaaaagcctt
     6361 tcaaaagtaa gtactttcgc ttacttctcc tctacctgtt tcggacgata aagctgacga
     6421 taatactcca gacgttgtag atataacggc acgctttcat caggtattcc ggacggaatc
     6481 gcgttacggg gaggtagttt tttcagatac tcccggaacg cctggctcga tgccatgaaa
     6541 tctacggctt tatcgataag aagcggaacg ttttcaccta atttgcccat gatgatatct
     6601 ccgtattacc ccgcgccggg aatgcgcggc cgccaatttt agtttagatc ccggtataaa
     6661 tgacattaag gaaaacgtgc tcaatcctca ataatcacct gcttctgttg actatctttt
     6721 ttctttaaat ttcttaatta atcaataaat tgcgtagttt ttaagcatgt taatgtttac
     6781 gttatagaac tattgcaatg tcatcagttg tgaacaaagc acctggtcgc gcatactagg
     6841 ggctataaat ttatctttat cagaagccat cacatgaaag aagtcgaaaa aaacgaaatc
     6901 aaacgtctca gcgatcgtct ggacgccatc cgccaccagc aggccgatct gtcgctggtt
     6961 gaagccgcag acaaatatgc cgaactggaa aaagagaaag ccactctgga agcagaaatt
     7021 gctcgcctgc gcgaagttca cagccagaaa ctcagtaagg aagcacaaaa gctgatgaag
     7081 atgccattcc agcgcgcgat taccaaaaaa gagcaggctg atatgggcaa gctgaagaaa
     7141 agtgttcgcg gactggtcgt tgtgcaccca atgaccgcgc tgggccgcga aatgggcctg
     7201 caagagatga cagggttctc aaagactgcg ttttaagttc ctttctcgtc gctgtaatca
     7261 cagcctctca ccatttactc gctcctgctg gcacatgcag gagcctgttt cagagatcga
     7321 atatttataa cgttcttaac attatatatt caatctcctc tattcttttt agcgcacatg
     7381 ccaggctaaa ttctgaaaat caggtacaag ttaaagaacg atgtatcaat attaacaatg
     7441 aattgccgca tatcaatgct tgaaacataa ttaattattt aaatttttct atcagcatcg
     7501 caaacttcgc aataaaacat cactcaactg aatatttacc tttccctgcc ctgggccttt
     7561 atccggtgaa acatttgaca ttgattgaca ccgatcatta aaatccgcgc gattaatatt
     7621 attggctaca aaccgaaatt gtttgacact taataacata ttcgcacaac cgattaatac
     7681 aaaaattctc taacaaattt atttgtcgga tatgctccac tctaatcatt cataaaaaaa
     7741 atgattgtgc cgtaattatc aactgtatcg ggattatgaa aaggaattca tcctatgaac
     7801 ctaaagaaaa cactgttaag cgtgttaata atagtgcctc tctgcttatt ggcagggtgt
     7861 gactatattg aaaaagcgag taaggtcgac gacctcgtta cacagcaaga gttgcaaaaa
     7921 agcaaaattg aggcgcttga gaaacaacag gaactcgata agcgaaagat agaacatttt
     7981 gaaaaacaac aaactactat cataaatagt accaagacgc tcgctggcgt ggtgaaggcg
     8041 gttaaagaca aacaggacga atttgtcttt acagaattta acccggcaca aacccaatac
     8101 ttcattttaa ataacggctc tgttggtttg gcaggccggg tgctggctat tgatgcagtt
     8161 gaaaatggca gcgttattcg tatttcgctg gttaatttat taagcgttcc cgtatcaaat
     8221 attggctttc atgcgacatg gggcaatgaa aggcccaccg atgccaaagc tctggcaaag
     8281 tggcaacaat tacttttcaa caccacactg aattccactc tgcaattaat gcctggtcaa
     8341 tggcaagaca ttaatctgac attaaaaggc gtttcgccta ataacctgaa gtatctgaag
     8401 ttgtccatca atatggccaa tctccagttc gacactgttc aatctgctga aactcggcag
     8461 cgaaaaaaca aaaaataaat gcagcttctg cgcttatatc aaggaataga gagcatcaat
     8521 aatgaacaaa atttttaaag ttatctggaa tccagcgacg ggcagttaca gcgttgccag
     8581 cgaaacggcg aagagccgtg gtaaaaaaag cgggcgcagt aagctgttaa tttctgtact
     8641 agttgcgagt gggttgttgt cgtcgtttgg agcgttagct aataactacg acgggcagcc
     8701 aactgattat ggcgatggct cagcaggtga cggctgggtt gctatcggta aaggggcaaa
     8761 agcaaatacc tttatgaaca ctagtggcgc gagtacagct ttaggatatg acgcgatagc
     8821 cgaaggtgag tacagttctg ccatcgggtc aaaaaccctt gcaactggtg gagcatccat
     8881 ggcgttcggg gttagtgcaa gagcaatggg tgacagaagt gtcgcgctgg gtgcatcgtc
     8941 agtagcaaat ggcgatcgtt cgatggcttt tggtcgttac gcaaagacga atggttatac
     9001 atctcttgct attggggact cctcccttgc cgatggtgaa aaaactattg cgttaggaaa
     9061 tacggctaaa gcttacgaaa ttatgagcat cgccctcggt gatcatgcca atgcgtcaaa
     9121 agaatattca atggcgctgg gtgcaagtag tgccgcatct gcggctaacg cgattgcagt
     9181 aggccgcaac agtgctgcag ctggcgtaga cagcctcgca ttcggtagac tatccgccgc
     9241 caacgccgca aacgcgattg ctatgggtgc agagagcaaa gctgctgaaa atgcgacggc
     9301 agttggtact aacgcggagg caaatggact taatagtatc gcgttgggct ccggcagtat
     9361 tgcagatgtc gacaacacta ttgctctggg caatcaaagt caggcagtag cggctggcgc
     9421 aattgccatc ggtcaaggga ataaagctga cggcgcaaat gctatcgcgc tgggtaatgg
     9481 tagtattaca ggtggtgtaa atgctattgc tcttggacaa ggcagttatg ccggtttaga
     9541 aaatggcact gcaattggtg ctcaagccag tgctcagggg aaaaattcag ttgctctggg
     9601 tgctggttct gtagcgactg acgcggatac tgtttctgtg ggtaacacaa cagctcagcg
     9661 acaaattgtc aatatggcag caggtgatat cagcactacc agtactgatg ccatcaatgg
     9721 atcacagctt tatgctatca gtaagtcagt agcggacaat cttggtgggg gggctaccgt
     9781 caatgcgcaa ggcgtcgtta cttccccaaa ttacaggctg aaaagtggta ttttcggcac
     9841 tgttggcgac gccttaacgg gcctggacaa taatacgtta caatgggact cccttaaaaa
     9901 ggcatatagt gcggcacatg gtacagatac taccagtacc atcaccaacg ttaaagacgg
     9961 cgctatttct gataccagta aggatgcggt taacggttct cagctgaaaa ccaccaacga
    10021 taacgtagcg accaatactg ccaatatcac caccaacact aacagtatca ataccctgac
    10081 ggattccgtt ggcgacctta aagacgatgc cctgctgtgg aatggcaccg cgttcagcgc
    10141 cgcgcacggc accgaagcca ccagcaaaat caccaacgtc aaagacggtg acctgacggc
    10201 tggcagcacc gacgcggtta acggctctca gctcaaaacc actaacgatg ccgtggcagc
    10261 caacaccacc aatatcgcca cgaacaccac caatatcgcc acgaacacca ccaacatcac
    10321 caacctgact gacgctgttg acagtctcgg tgatgactct ctgctgtgga atgatgcagc
    10381 gaaagcattc agtgccgctc acggtaccga tgccaccagc aaaatcacta acgtcaaaga
    10441 cggtgacctg acggctggca gcaccgacgc ggttaacggc tctcagctca aaaccactaa
    10501 cgatgccgtg gcagccaaca ccaccaatat cgccacgaac ac
//
LOCUS       DAZGQL010000145        10344 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.145, whole genome shotgun sequence.
ACCESSION   DAZGQL010000145 DAZGQL010000000
VERSION     DAZGQL010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10344
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.145"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            178..1572
                     /gene="galP"
                     /locus_tag="ACHKIR_004638"
     CDS             178..1572
                     /gene="galP"
                     /locus_tag="ACHKIR_004638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HFO9992283.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            1649..2146
                     /locus_tag="ACHKIR_004639"
     CDS             1649..2146
                     /locus_tag="ACHKIR_004639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HFO9992284.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            2241..2948
                     /gene="endA"
                     /locus_tag="ACHKIR_004640"
     CDS             2241..2948
                     /gene="endA"
                     /locus_tag="ACHKIR_004640"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HFO9992285.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWDKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            3028..3759
                     /gene="rsmE"
                     /locus_tag="ACHKIR_004641"
     CDS             3028..3759
                     /gene="rsmE"
                     /locus_tag="ACHKIR_004641"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFO9992286.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            3772..4722
                     /gene="gshB"
                     /locus_tag="ACHKIR_004642"
     CDS             3772..4722
                     /gene="gshB"
                     /locus_tag="ACHKIR_004642"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HFO9992287.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            4831..5394
                     /locus_tag="ACHKIR_004643"
     CDS             4831..5394
                     /locus_tag="ACHKIR_004643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HFO9992288.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            5394..5810
                     /gene="ruvX"
                     /locus_tag="ACHKIR_004644"
     CDS             5394..5810
                     /gene="ruvX"
                     /locus_tag="ACHKIR_004644"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFO9992289.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNI
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(5984..6964)
                     /locus_tag="ACHKIR_004645"
     CDS             complement(5984..6964)
                     /locus_tag="ACHKIR_004645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HFO9992290.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRTILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQRVKEGRL"
     gene            6982..7686
                     /gene="yggS"
                     /locus_tag="ACHKIR_004646"
     CDS             6982..7686
                     /gene="yggS"
                     /locus_tag="ACHKIR_004646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HFO9992291.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLDVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            7704..8270
                     /gene="yggT"
                     /locus_tag="ACHKIR_004647"
     CDS             7704..8270
                     /gene="yggT"
                     /locus_tag="ACHKIR_004647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HFO9992292.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            8267..8557
                     /gene="yggU"
                     /locus_tag="ACHKIR_004648"
     CDS             8267..8557
                     /gene="yggU"
                     /locus_tag="ACHKIR_004648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein YggU"
                     /protein_id="HFO9992293.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            8565..9158
                     /locus_tag="ACHKIR_004649"
     CDS             8565..9158
                     /locus_tag="ACHKIR_004649"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HFO9992294.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETMKDVPDDQRQARFHCVLIYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            9151..10287
                     /gene="hemW"
                     /locus_tag="ACHKIR_004650"
     CDS             9151..10287
                     /gene="hemW"
                     /locus_tag="ACHKIR_004650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFO9992295.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     NDLDNDVAYAQGREVQTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFSQYTGLS
                     EEVIRSQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
BASE COUNT         2431 a         2720 c         2721 g         2472 t
ORIGIN      
        1 tgcagataac agcgtttaat ctatgatgat ataactcaat tattttcatg cacttaaatc
       61 ataaccaaga taaatgttag tgtaagcgat tacactgatg tgatttgctt cacatctttt
      121 tacgtcgtac tcacctatct taattcacaa taaaaaataa ccatattgga gggcatcatg
      181 cctgacgcta aaaaacaggg gcggtcaaac aaggcaatga cgtttttcgt ctgcttcctt
      241 gccgctctgg cgggattact ctttggcctg gatatcggtg taattgctgg cgcactgccg
      301 tttattgcag atgaattcca gattacttcg cacacgcaag aatgggtcgt aagctccatg
      361 atgttcggtg cggcagtcgg tgcggtgggc agtggctggc tctcctttaa actcgggcgc
      421 aaaaagagcc tgatgatcgg cgcaattctg tttgttgccg gttcactgtt ctctgcggct
      481 gcgccaaacg ttgaagtact gattctttcc cgcgttctgc tggggttggc ggtgggtgtg
      541 gcttcttata ccgcaccgct gtacctctct gaaattgcgc cggaaaaaat tcgcggcagc
      601 atgatctcga tgtatcagtt gatgatcact atcgggatcc tcggtgctta tctttctgat
      661 accgccttca gctacaccgg tgcatggcgc tggatgctgg gtgtgattat catcccggca
      721 attttgctgc tgattggtgt cttcttcctg ccagacagtc ctcgttggtt tgccgccaaa
      781 cgccgttttg ttgatgccga acgcgtgctg ctacgcctgc gtgacaccag cgcggaagcg
      841 aaacgcgaac tggatgaaat ccgtgaaagt ttgcaggtta aacagagtgg ctgggcgctg
      901 tttaaagaga acagcaactt ccgccgcgcg gtgttccttg gcgtactgtt gcaggtaatg
      961 cagcaattca ccgggatgaa cgtcatcatg tattacgcgc cgaaaatctt cgaactggcg
     1021 ggttatacca acaccaccga gcaaatgtgg gggacggtga tcgtcggcct gaccaacgta
     1081 ctcgccacct ttatcgcaat cggccttgtt gaccgctggg gacgtaaacc aacgctaacg
     1141 ctgggcttcc tggtgatggc tgctggcatg ggcgtactcg gtacaatgat gcatatcggt
     1201 atccactctc cgtcggcgca gtatttcgcc atcgccatgc tgctgatgtt tattgtcggt
     1261 tttgccatga gtgccggtcc gctgatttgg gtactgtgct ccgaaattca gccgctgaaa
     1321 ggccgcgatt ttggcatcac ctgctccacc gccaccaact ggattgccaa catgatcgtt
     1381 ggcgcaacgt tcctgaccat gctcaacacg ctgggtaacg ccaacacctt ctgggtgtat
     1441 gcggctctga acgtactgtt tatcttgctg acattgtggc tggtaccgga aaccaaacac
     1501 gtttcgctgg aacatattga acgtaatctg atgaaaggtc gtaaactgcg cgaaatcggc
     1561 gctcacgatt aatctcctca ggcttcctcc cgtcgcggag gaagccacct cttgcagtca
     1621 tcttttcttc gctctatcct ctgccgctat gaaaacatcc cgtctcccta tcgccatcca
     1681 gcaggccgtt atgcgccgcc tgcgggaaaa actcgcccag gccaacctga agctagggcg
     1741 taactacccg gagccaaaac tctcttacac ccagcgcgga acctccgccg gaacagcctg
     1801 gctggaaagc tatgaaatcc gcctcaatcc cgttttgctg ttggaaaaca gtgaagcttt
     1861 tattgaagaa gtggtaccgc acgaactggc acatttgctg gtatggaaac atttcggccg
     1921 cgtagcgcca catggcaaag agtggaagtg gatgatggaa agcgtgctgg gcgttcccgc
     1981 ccgtcgtacg catcagttcg aactgcaatc cgtgcgtcgc aacaccttcc cctaccgctg
     2041 caagtgccag gagcatcagc ttaccgtacg ccgccataat cgcgtagttc gtggcgaggc
     2101 cgtctatcgc tgtgttcact gcggtgaaca gctggttgcg aaataaccac ctgaactatc
     2161 aggaactttc ctgatctggc tgattgcata ccaaaacaac tttcgctacg ttgctggctc
     2221 gttttaacac ggagtaagtg atgtaccgtt atttgtctat tgctgcggtg gtactgagcg
     2281 cagcattttc cggcccggcg ttggccgaag gtatcaatag tttttctcag gcgaaagccg
     2341 cggcggtaaa agtccacgct gatgcgcccg gtacgtttta ttgcggatgt aaaattaact
     2401 ggcagggcaa aaaaggcgtt gttgatctgc aatcgtgcgg ctatcaggtg cgcaaaaatg
     2461 aaaaccgcgc cagccgcgta gagtgggaac acgtcgttcc cgcctggcaa ttcggtcacc
     2521 agcgccagtg ctggcaggac ggtggacgta aaaactgcgc caaagatccg gtctatcgca
     2581 agatggaaag cgatatgcat aacctgcagc cgtcagtcgg tgaggtgaat ggcgatcgcg
     2641 gcaactttat gtacagccag tggaatggcg gcgaaggcca gtacggtcaa tgcgccatga
     2701 aggtcgattt caaagaaaaa gccgccgaac caccagcgcg tgcacgcggt gccattgcgc
     2761 gcacctactt ctatatgcgt gaccaataca acctgacact ctctcgccag caaacgcaac
     2821 tgttcaacgc atgggacaag atgtatccgg ttaccgactg ggagtgcgag cgcgatgaac
     2881 gcatcgcgaa ggtgcagggc aatcataacc cgtatgtgca acgcgcttgc caggcgcgaa
     2941 agagctaacc tacactagcg ggattctttt tgttaacccc taccccacgc gtacaaccgc
     3001 gtggggagac gacgcggatt tttaactatg cgtatccccc gcatttatca tcctgaacca
     3061 ctgaccagcc attctcacat cgcgctttgc gaagatgccg ccaaccatat cgggcgcgta
     3121 ctgcgtatgg ggccggggca ggcgttgcaa ttgtttgacg gtagcaacca ggtctttgac
     3181 gccgaaatta ccagcgccag caaaaaaagc gtggaagtga aggtactgga aggccagatc
     3241 gacgatcgcg aatctccgct gcatattcac ctcggtcagg tgatgtcgcg tggtgaaaaa
     3301 atggaattta cgatccagaa atcgatcgaa ctcggtgtaa gcctcattac gccacttttt
     3361 tctgagcgct gcggcgttaa actggatagt gaacgtctga acaagaagct ccagcagtgg
     3421 cagaagattg caattgctgc ctgtgagcag tgtggtcgta accgggtgcc ggaaatccgt
     3481 ccagcgatgg atctggaagc ctggtgtgca gagcaggatg aaggactgaa actgaatctt
     3541 cacccgcgcg ccagtaacag catcaatacg ttgccgttac cggttgaacg tgtccgcctg
     3601 ctgattggcc cggaaggcgg tttatcggca gatgaaattg ccatgactgc ccgctatcaa
     3661 tttactgata tcctgttggg acctcgcgtt ttgcgtacag agacaactgc gctcaccgcc
     3721 attaccgcgc tacaagtacg atttggcgat ttgggctaac ggagaagaat aatgatcaag
     3781 ctcggcatcg tgatggaccc catcgcaaac atcaacatca agaaagattc cagtttcgct
     3841 atgttgctgg aagcacagcg tcgtggttac gaacttcact atatggagat gggcgatctg
     3901 tatctgatca atggcgaagc ccgcgcccat acccgcacgc tgaacgtgaa gcagaactac
     3961 gaagagtggt tttcattcgt cggtgaacag gatctgccgc tggccgatct cgatgtgatc
     4021 ctgatgcgta aagacccgcc gtttgatacc gagtttatct acgcgaccta tattctggaa
     4081 cgtgccgaag agaaagggac gctgatcgtt aacaagccgc agagcctgcg cgactgtaac
     4141 gagaaactgt ttaccgcctg gttctctgac ttaacgccag aaacgctggt tacgcgcaat
     4201 aaagcgcagc taaaagcgtt ctgggagaaa cacagcgaca tcattcttaa gccgctggac
     4261 ggtatgggcg gcgcgtcgat tttccgcgtg aaagaaggcg atccaaacct cggcgtgatt
     4321 gccgaaaccc tgactgagca tggcactcgc tactgcatgg cgcaaaatta cctgccagcc
     4381 attaaagatg gcgacaaacg cgtgctggtg gtggatggcg agccggtacc gtactgcctg
     4441 gcgcgtattc cgcagggggg cgaaacccgt ggcaatctgg ctgccggtgg tcgcggtgaa
     4501 cctcgtccgc tgacggaaag tgactggaaa atcgcccgtc agatcgggcc gacgctgaaa
     4561 gaaaaagggc tgatttttgt tggtctggat atcattggcg accgtctgac tgaaattaac
     4621 gtcaccagcc caacctgtat tcgtgagatt gaagcagagt ttccggtgtc gatcaccgga
     4681 atgttaatgg atgccatcga agcacgttta cagcagcagt aacccacctt agcgagaagg
     4741 atctcgttga gactctgagt gacagcgccc ttctttccac gcatactggg cgctgttgct
     4801 tttttgaacc aggaaacaga acctctgaca atgaatttac agcatcactt tcttattgcc
     4861 atgcctgctc tccaggatcc gattttccgt cgttccgtgg tctacatttg cgaacataat
     4921 accaatggtg caatggggat catcgtcaac aagccgctgg aaaatctcaa aattgaaggg
     4981 attctggaaa agctgaagat cacgccggag ccgcgtgatg aatcaatccg tctggataaa
     5041 ccggttatgc tcggtggtcc gctggctgaa gatcgcgggt ttattttgca tactccgccc
     5101 tctaattttg cttccagcat tcgcatttca gacaacacgg tgatgaccac ttcccgcgat
     5161 gtgctggaaa cgctcggcac cgataaacaa ccgtctgacg tattggtggc tctgggctat
     5221 gcctcctggg agaaaggtca actggaacaa gaaattctcg ataacgcgtg gctaacggcc
     5281 ccggcggatc tgaatattct gttcaaaaca ccgattgccg accgctggcg cgaggcggca
     5341 aaactgattg gtgtggatat tctcaccatg cctggtgtgg cagggcacgc ctgatgagtg
     5401 gaaccttact cgccttcgac ttcggtacca aaagcattgg tgtagcggtc ggccaacgca
     5461 ttaccggcac cgctcgccca ttgcctgcaa ttaaagcaca ggacggtacg ccggactgga
     5521 acattatcga gcgtttactg aaagagtggc agcctgacga aatcatcgtc ggtttgccgc
     5581 tgaatatgga cggcaccgaa cagccgttaa ccgccagagc gcgtaaattt gccaaccgta
     5641 ttcatggtcg tttcggtgtt gaagtaaagc tccatgacga gcgtcttagc accgtggagg
     5701 cacgttccgg tctgtttgaa cagggcggct atcgggcgct caacaaaggc aaagtcgact
     5761 ctgcctctgc ggttattatt ctcgaaagct atttcgagca gggatattaa ggcgatttac
     5821 tagcgcacag atttctgaca atgtgctcgt tgtttcatgt tggatgcggc gtaaacgcct
     5881 tatccaacct acagtgagtg cgaattcaaa gttacgcttc ccttgtaggc ccgataagct
     5941 tgcgcatcgg gcatatttac aattcgtatc agacacagca acgtcacaag cgcccttctt
     6001 tcacccgctg ctgataactc tgctgaaacg ttatcatccc cacctgctgc ccggtttgaa
     6061 taacatgcgg taactggtgg gttttccctt cacgaatcaa attccccacc gcgggtgtgt
     6121 taatcagcaa ttcaaacaac gccacgcgtc cttcctgttt atccacttcc agcttttgtg
     6181 acaacaccgc ccgtaaacta cctgccagtt gattacgcac gggatctttt tcctgcgccg
     6241 gaaatgaatc taccagtcgc tcaactgcct gcgccgcacc acgcgtatgt aatgttgcca
     6301 gcaccaaatg cccggtttct gccgccgtca gcgccagacg gattgtctcg ctgtcacgca
     6361 gctctccgag caaaatcaca tcaggatctt cccgcaatgc agcccgcaat cccgatgcga
     6421 acgtcataca atgcaaaccg atttcccgct gctggatcaa acatcgctgg cttgcataga
     6481 gatattcgac aggatcttcc agcgtcagaa tatgcgcatc ggcatgttga ttgagatagc
     6541 caaccatcgc cgccagcgtg gtagatttgc cgctccccgt cgcccccgtc accagaatca
     6601 ggccattctc gctcttgagt aattccggca ataccggtgg cgcaccaagc tgttcgagct
     6661 gtggacagtg cgaaggtaac aaccgtaatg ccagagaaat accttgccgt tgcgcgaacg
     6721 cactgccacg caaccgctgg ttttccgcca gcgacacagc aaaatccaac tgaccattct
     6781 ccagcaatat tgtccgctga tcgtcatcca gccactcccg cagtagctct tcgacgtccg
     6841 gcgcatcaaa cggcgcagct tccattcgcc cgcgaatgcg ccatcgtgcg ggccaggcgc
     6901 tgcacaggtg tagatccgag acgttatgct ttacactaag ggccacaatt tcttccatat
     6961 tcatactaag atcctcggaa aatgaacgat attgcgcata acctggcaca ggtccgggac
     7021 aaaatctcag cggctgcaac gcgttgcggc cgttctccag aagaaattac gctgcttgca
     7081 gtcagtaaaa caaaacctgc gagcgccatc gcagaagcca ttgatgccgg gcagcgtcaa
     7141 tttggtgaaa actacgttca ggaaggggta gataaaattc gccactttca ggaactgggc
     7201 gtaacaggat tagaatggca ttttattggc ccgttgcagt ctaataaaag ccgcctggtg
     7261 gcagagcatt tcgactggtg tcataccatc gaccgtttgc gcattgctac ccgcctcaac
     7321 gatcagcgcc cggcagaact tccccctctt gacgtactga ttcaaattaa tattagtgat
     7381 gaaaacagta agtccgggat tcaactggca gaactggacg agctggcagc tgcggtcgct
     7441 gaactaccgc gtttacgtct gcgcgggctg atggcaatcc ctgcgcctga gtcagaatat
     7501 gtaaggcagt ttgaagttgc acgccaaatg gctgtagcat ttgccggact gaaaacgcgc
     7561 tacccgcata tcgacacgct ctctctggga atgtcggacg atatggaagc cgccattgcg
     7621 gcaggtagca cgatggttcg tatcggcact gcaatttttg gtgcgcgtga ttactctaaa
     7681 aaataaggaa ttaaaggaac gccatgaata cgttgacttt cctgctttca acggtcattg
     7741 agctgtatac catggtgctg ttattacgca tctggatgca gtgggctcat tgtgattttt
     7801 acaacccctt ctcacagttt gtagtgaagg taacgcagcc aattatcggg ccactgcgcc
     7861 gcgttattcc ggcaatggga cccattgaca gcgcctcgct gctggttgcc tatattctca
     7921 gttttatcaa agccatcgtg ctgtttaaag tggtgacctt cctgccaatc atctggattg
     7981 ccggtttact gattctgctg aaaaccatcg gcctgctgat tttctgggtc ctgctggtga
     8041 tggcgattat gagctgggta agccagggtc gtagtccgat tgaatacgtg ctgattcagc
     8101 ttgccgatcc gctgctgcgc ccgattcgcc gcctgctacc ggcaatgggt gggattgatt
     8161 tctcgccgat gatcctcgtt ctgctgctgt atgtcatcaa tatgggtgtc gcagaagtat
     8221 tacaggcaac cggaaacatg ctgctgccgg ggctgtggat ggcgttatga gtgccgtaac
     8281 agttaatgat gacggtctgg ttttacggct ctatattcag ccgaaagcca gccgtgattc
     8341 tattgtcggt ttacatggcg acgaggtaaa agtcgccatt accgcgccgc cggttgacgg
     8401 ccaggccaac agccatctgg tgaagtttct cggtaagcaa ttccgggttg ccaaaagcca
     8461 ggtggtgatt gaaaaaggcg aacttggccg ccacaaacaa attaaaatca ttaatccgca
     8521 acaaatcccg ccagaaatcg cggcgttaat taattaggta tcctatgcaa aaagttgtcc
     8581 tcgcaaccgg caatgccggt aaagtgcgtg agctggcgtc gctgcttagc gacttcggtc
     8641 ttgatatcgt ggcccaaaca gacctcggcg tagattccgc tgaagaaacc ggcctgacct
     8701 ttatcgaaaa cgcgattctg aaagcgcgcc atgcggcaaa agtgaccggt ttaccggcaa
     8761 ttgccgacga ctccggtctg gcggtagatg cgctgggtgg cgcgccgggt atttactccg
     8821 cgcgttattc cggtgaagac gcgaccgatc aaaagaacct gcaaaaactg ctggaaacaa
     8881 tgaaagacgt accggacgac caacgtcagg cgcgtttcca ctgcgtgctg atatatctgc
     8941 gtcacgcgga agatccgact ccgctggtgt gccacggtag ctggccgggc gtgattactc
     9001 gtgaaccagc gggtactggt ggctttggtt atgatccaat tttcttcgtt ccttctgaag
     9061 ggaaaaccgc tgccgaactg acccgcgaag aaaagagcgc catttcccac cgtggtcagg
     9121 cattgaaact gctgctggac gctttacgta atggttaaat taccgccgct gagtctctac
     9181 attcacatcc cgtggtgcgt gcagaaatgc ccgtactgcg atttcaactc tcacgcgttg
     9241 aaaggagaag tgccgcacga cgattatgtt cagcatctgc ttaacgatct ggacaacgat
     9301 gtggcttacg ctcagggccg tgaagtgcag acaatcttta ttggcggtgg tacgccgagc
     9361 ctgctttccg gcccggcgat gcaaacgctg ctggacggcg tgcgtgcgcg tttgccgctg
     9421 gcagcggatg cagaaattac gatggaagcg aacccaggca cggtagaagc ggatcgcttt
     9481 gtcgattatc agcgtgctgg tgtgaaccgc atctctattg gtgtgcagag ttttagcgaa
     9541 gaaaagctga aacgactcgg gcgtattcat ggcccgcaag aagcgaaacg agcggcaaag
     9601 ctggcgagcg gtttagggtt acgtagtttt aaccttgatt tgatgcatgg gctgccggat
     9661 caatcactgg aagaggcgct aggcgatctg cgccaggcta ttgaactgaa tccgccacat
     9721 ctttcctggt atcaactgac catcgaacct aatacgctgt ttggctcgcg ccctcctgta
     9781 ctgccggacg atgacgcgct gtgggatatt ttcgaacagg ggcatcagtt attaaccgca
     9841 gcgggctatc agcagtatga aacatccgct tacgccaaac ccggttatca gtgccagcac
     9901 aatctcaact actggcgatt tggcgactac atcggtattg gctgcggcgc gcacggcaaa
     9961 gtgaccttcc cggatgggcg cattctgcgt accaccaaaa cgcgtcatcc gcgtggtttt
    10021 atgcagggaa gatatctgga aagccagcgt gatgtcgaag ctgcagataa accgtttgag
    10081 ttctttatga atcgcttccg tctgcttgag gccgcgccgc gcgtggagtt tagtcagtat
    10141 actggccttt ctgaagaagt tattcgctct cagttagacg aggctatcgc tcagggttat
    10201 ctcacagaat gtgcggatta ctggcagata accgagcatg gtaaattatt cttaaactca
    10261 cttcttgaat tattcctcgc tgagtaatca ttaatgcgcc tgcgggcgca ttaatcactt
    10321 cttttacccc cccccgttaa ttaa
//
LOCUS       DAZGQL010000146        10299 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.146, whole genome shotgun sequence.
ACCESSION   DAZGQL010000146 DAZGQL010000000
VERSION     DAZGQL010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10299
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.146"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            332..4582
                     /gene="fdeC"
                     /locus_tag="ACHKIR_004651"
     CDS             332..4582
                     /gene="fdeC"
                     /locus_tag="ACHKIR_004651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000092542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin FdeC"
                     /protein_id="HFO9992296.1"
                     /translation="MSRYKTDNKQPRFRYSVLARCVAWANISVQVLFPLAVTFTPVMA
                     ARAQHAVQPRLSMENTTVTADNNVEKNVASLAANAGTFLSSQPDSDATRNFITGMATA
                     KANQEIQEWLGKYGTARVKLNVDKNFSLKDSSLEMLYPIYDTPTNMLFTQGAIHRTDD
                     RTQSNIGFGWRHFSENDWMAGVNTFIDHDLSRSHTRIGVGAEYWRDYLKLSANGYIRA
                     SGWKKSPDVEDYQERPANGWDIRAEGYLPAWPQLGASLMYEQYYGDEVGLFGKDKRQK
                     DPHAITAEVNYTPVPLLTLSAGHKQGKSGENDTRFGLEVNYRIGEPLEKQLDTDSIRE
                     RRMLAGSRYDLVERNNNIVLEYRKSEVIRIALPERIEGKGGQTVSLGLVVSKATHGLK
                     NVQWEAPSLLAAGGKITGQGNQWQVTLPAYQAGKDNYYAISAIAYDNKGNASKRVQTE
                     VVISGAGMSADRTALTLDGQSRIQMLANGNEQKPLVLSLRDAEGQPVTGMKDQIKTEL
                     TFKPAGNIVTRTLKATKSQAQPTLGEFTETEAGVYQSVFTTGTQSGEATITVSVDDMS
                     KTVTAELRATMMDVANSTLSANEPSGDVVADGQQAYTLTLTAVDSEGNPVTGEASRLR
                     LVPQDTNGITVGAISEIKPGVYSATVSSTRAGNVVVRAFSEQYQLGTLQQTLKFVAGP
                     LDAAHSSITLNPDKPVVGGTVTAIWTAKDANDNPVTGLNPDAPSLSGAAAAGSTASGW
                     TDNGDGTWTAQISLGTTAGELDVMPKLNGQDAAANAAKVTVVADALSSNQSKVSVAED
                     HVKAGESTTVTLVAKDAHGNAISGLSLSASLTGTASEGATVSSWTEKGDGSYVATLTT
                     GGKTGELRVMPLFNGQPAATEAAQLTVIAGEMSSANSTLVADNKTPTVKTTTELTFTV
                     KDAYGNPVTGLKPDAPVFSGAASTGSERPSAGNWTEKGNGVYVSTLTLGSAAGQLSVM
                     PRVNGQNAVAQPLVLNVAGDASKAEIRDMTVKVNNQLANGQSANQITLTVVDSYGNPL
                     QGQEVTLTLPQGVTSKTGNTVTTNAAGKVDIELMSTVAGELEIEALVKNSQKTVKVKF
                     KADFSTGQASLEVDAAAQKVANGKDAFTLTATVKDQYGNLLPGAVVVFNLPRGVKPLA
                     DGNIMVNADKEGKAELKVVSVTAGTYEITASAGNDQPSNAQSVTFVADKTTATISSIE
                     VIGNRAVADGKTKQTYKVTVTDANNNLLKDSEVTLTASPAKLVLDPNGTATTNEQGQA
                     IFTATTTVAATYTLTAQVSQNNGQVSTKTAESKFVADDKNAVLAASPERVDSLVADGK
                     TTATLTVTLMSGVNPVGGTMWVDIEAPEGVTEADYQFLPSKNDHFASGKITRTFSTNK
                     PGTYTFTFNSLTYGGYEMKPVTVTINAVPADTEGAEEK"
     gene            complement(4707..5564)
                     /locus_tag="ACHKIR_004652"
     CDS             complement(4707..5564)
                     /locus_tag="ACHKIR_004652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO9992297.1"
                     /translation="MIRQKILQQLLEWIECNLEHPISIEDIAQKSGYSRRNIQLLFRN
                     FMHVPLGEYIRKRRLCRAAILVRLSAKSMLDIALSLHFDSQQSFSREFKKLFGCSPRE
                     YRHRDYWDLANIFPSFLIRQQQKTECRLVNFPETPIFGNSFKYDIEVSNKSPDEEVKL
                     RRHHLARCMKSFKTDIYFVSTFEPSTKSVDLLTVETFAGTVCKQTDSEMSKEWTINRG
                     LYASFRYEGEWEHYPEWARNLYLMELPARGLARMNGSDIERFYYNENFVEIDSNNIVC
                     EIFIPVRPV"
     gene            5813..6682
                     /locus_tag="ACHKIR_004653"
     CDS             5813..6682
                     /locus_tag="ACHKIR_004653"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFO9992298.1"
                     /translation="MEFSVLSNNLKMPMMGFGVFQVTDKNVCKQSVLNAIRTGYRLID
                     TAAVYGNEDAVGEAVREAISEGLCTREELFITSKLWVQDMLNQDIAAAGIEASLKKSG
                     LEYFDLYLLHQAMRDYFSAWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMV
                     NQVELHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQV
                     ILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGESVKHFNP
                     EFVRGCLAVKIHD"
     gene            complement(6842..7435)
                     /gene="rclC"
                     /locus_tag="ACHKIR_004654"
     CDS             complement(6842..7435)
                     /gene="rclC"
                     /locus_tag="ACHKIR_004654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HFO9992299.1"
                     /translation="MERYLHLLSRGDKIGLTLIRLSIAIIFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQHLTHEGEYKPEARAWQTANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAFTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSARDILKQRSNESSSTLKTEY"
     gene            complement(7447..7683)
                     /gene="rclB"
                     /locus_tag="ACHKIR_004655"
     CDS             complement(7447..7683)
                     /gene="rclB"
                     /locus_tag="ACHKIR_004655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance periplasmic protein
                     RclB"
                     /protein_id="HFO9992300.1"
                     /translation="MFKKSVLFATLLSGVMAFSTNADDKIILKHISVSSVSASPTVLE
                     NAIADMARKYNASSWKVTSMRIDNNSTATAVLYK"
     gene            complement(7792..9117)
                     /gene="rclA"
                     /locus_tag="ACHKIR_004656"
     CDS             complement(7792..9117)
                     /gene="rclA"
                     /locus_tag="ACHKIR_004656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance oxidoreductase
                     RclA"
                     /protein_id="HFO9992301.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADIPNIDVIDGQAEFI
                     NNHSLRVHRPGGNLEIHGEKIFINTGAQAVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREDRDIADNIATILRDQGVDIIL
                     NAHVERISHHENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGIAVNERGAIVV
                     DKRLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGKRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLVK"
     gene            9343..10197
                     /gene="rclR"
                     /locus_tag="ACHKIR_004657"
     CDS             9343..10197
                     /gene="rclR"
                     /locus_tag="ACHKIR_004657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine-specific transcriptional
                     regulator RclR"
                     /protein_id="HFO9992302.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLPHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFVAPVNHSVEYNWLREAIPFLQQESRSAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSREMLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
BASE COUNT         2808 a         2293 c         2540 g         2658 t
ORIGIN      
        1 agcagttaat taaagacggg tcattattat tcactctgaa taatgaatta ttcactgccc
       61 gcagagtaag aaatataact taagtatcta tttaatgact tgcacaaaaa gctaaatttt
      121 tacccataaa taaaaatata atcccgcgcc caaccacctg atgagtggct acagacactg
      181 gatatattag gtggcggtgt actttcttac ataaaggtat ttccttttct gtggaaaagg
      241 aaaccgggaa atccccggtt tttctgacaa gcagacgcca ttatttgtgt ctgcctatgt
      301 tcgttaattc gttcatcagg aaattatctc aatgtcacgt tataaaacag acaataaaca
      361 gccacgattt cgttattcag ttctggcccg ctgcgtggcg tgggcaaata tctctgttca
      421 ggttcttttt ccactcgcag tcacctttac gccagtaatg gcagcacgtg cgcagcatgc
      481 ggttcagcca cggttgagca tggaaaatac tacggtaact gctgataata acgtggagaa
      541 aaatgtcgcg tcgcttgccg ctaatgccgg gacattttta agcagtcagc cagatagcga
      601 tgcgacacgt aactttatta ccggaatggc caccgctaaa gctaaccagg aaattcagga
      661 gtggctcggg aaatacggta ctgcgcgcgt caaactgaat gtcgataaaa atttctcgct
      721 gaaggactct tcgctggaaa tgctttatcc gatttatgat acaccgacaa atatgttgtt
      781 cactcagggg gcaatacatc gtaccgacga tcgtactcag tcaaatattg gttttggctg
      841 gcgtcatttt tcagaaaatg actggatggc gggggtgaat acttttatcg atcatgattt
      901 atcccgtagt catacccgca ttggtgttgg tgcggaatac tggcgtgatt atttgaaact
      961 gagcgccaat ggttatatcc gggcttctgg ctggaaaaaa tcgccggatg ttgaggatta
     1021 tcaggaacgc ccggcgaatg gctgggatat tcgtgctgag ggctatttac ctgcctggcc
     1081 gcagcttggc gcaagcctga tgtatgaaca gtattatggc gatgaagtcg ggctgtttgg
     1141 taaagataaa cgccagaaag atccacatgc gattaccgct gaagtgaatt acacgccagt
     1201 gcctcttctg accctgagtg ccgggcataa gcagggcaag agtggtgaga atgacactcg
     1261 ctttggcctg gaagttaatt atcggattgg cgaacctctg gaaaaacaac tcgatacaga
     1321 cagcattcgc gagcgtcgaa tgctggcagg cagccgctat gacctggttg agcgtaataa
     1381 caatatcgtt cttgagtatc gcaaatctga agtgatccgt attgctctgc ctgagcgtat
     1441 tgaaggcaag ggcggccaga cggtttccct ggggcttgtg gtcagcaaag caactcacgg
     1501 tctgaaaaat gtgcaatggg aagcgccgtc tttgctggcc gcaggcggaa aaattacggg
     1561 gcagggcaat cagtggcaag tgacgctccc ggcttatcag gcaggcaaag acaattatta
     1621 tgcgatttca gcgattgcct acgataacaa aggcaatgcc tcgaaacgtg tgcagacaga
     1681 agtggttatt agcggagctg gtatgagcgc cgatcgtacg gcgttaacgc ttgacggtca
     1741 gagccgtatt caaatgcttg ctaacggtaa tgagcaaaag ccgttggtgc tgtctctgcg
     1801 cgacgccgag ggtcagccag tcacgggcat gaaagatcag atcaagactg aactaacctt
     1861 caaaccggct ggaaatattg tgactcgtac cctgaaggcc actaaatcac aggcacagcc
     1921 aacactgggt gagttcaccg aaactgaagc aggggtgtat cagtctgtct ttactaccgg
     1981 aacgcagtca ggtgaggcaa cgattactgt tagcgttgat gacatgagca aaactgtcac
     2041 tgcagaactg cgggccacga tgatggatgt ggcaaactcc accctgagcg ctaacgagcc
     2101 gtcaggtgat gtggttgctg atggtcagca agcctacacg ctgacactga cagcggtgga
     2161 ctccgagggt aatccggtga cgggagaagc cagccgcctg cgacttgttc cgcaagacac
     2221 taatggtata accgttggtg ccatttcgga aataaaacca ggggtttaca gcgccacggt
     2281 ttcttcgacc cgtgccggaa acgttgttgt gcgtgccttc agcgagcagt atcagctggg
     2341 cacattacaa caaacgctga agtttgttgc cgggccgctt gatgcagcac attcgtccat
     2401 caccctgaat cctgataaac cggtggttgg cggtacagtt acggcaatct ggacggcaaa
     2461 agatgctaat gacaaccctg taactggcct caatccggat gcaccgtcat tatcgggcgc
     2521 agctgctgct ggttctacgg catcaggctg gacggataat ggcgacggga cctggactgc
     2581 gcagatttct ctcggcacta cggcgggtga attagacgtt atgccgaagc tcaatgggca
     2641 ggacgcggca gcaaatgcgg caaaagtaac cgtggtggct gatgcattat cttcaaacca
     2701 gtcgaaagtc tctgtcgcag aagatcacgt aaaagccggt gaaagcacaa ccgtaacgct
     2761 ggtggcgaaa gatgcgcatg gcaacgctat cagtggtctt tcgttgtcgg caagtttgac
     2821 ggggaccgcc tctgaagggg cgaccgtttc cagttggacc gaaaaaggtg acggttccta
     2881 tgttgctacg ttaactacag gcggaaagac gggcgagctt cgtgtcatgc cgctcttcaa
     2941 cggccagcct gcagccaccg aagccgcgca gctgactgtt attgccggag agatgtcatc
     3001 agcgaactct acgcttgttg cggacaataa aactccaacg gttaaaacga cgacggaact
     3061 caccttcacc gtgaaggatg cgtacgggaa tccggtcacc gggctgaagc cagatgcacc
     3121 agtgtttagt ggtgccgcca gcacggggag tgagcgtcct tcagcaggaa actggacaga
     3181 gaaaggtaat ggggtctacg tgtcgacctt aacgctggga tctgccgcgg gtcagttgtc
     3241 tgtgatgccg cgagtgaacg gccaaaatgc cgttgctcag ccactggtgc tgaatgttgc
     3301 aggtgacgca tctaaggctg agattcgtga tatgacagtg aaggttaata accaactggc
     3361 taatggacag tctgctaacc agataaccct gaccgtcgtg gacagctatg gtaacccgtt
     3421 gcaggggcaa gaagttacgc tgactttacc gcagggtgtg actagcaaga cggggaatac
     3481 agtaacaacc aatgcggcag gtaaagtgga cattgagctt atgtcaacgg ttgcagggga
     3541 acttgagatc gaggccttgg tgaaaaactc tcagaagacg gtcaaggtga aattcaaggc
     3601 ggatttcagt accggtcagg cgagcctgga ggtagacgcc gctgctcaaa aagtggcaaa
     3661 cggcaaagat gcctttacgc tgacggcaac ggttaaggat caatacggca accttcttcc
     3721 tggcgctgtg gtcgtcttta atctgcctcg gggcgtcaaa ccgcttgcag acggtaatat
     3781 catggtgaac gccgacaagg agggtaaagc ggaactgaaa gtggtttccg tgactgccgg
     3841 aacctatgag atcacggcgt cagcaggaaa tgaccagcct tcgaatgcgc agtctgtaac
     3901 gtttgtggct gataagacta cggcgaccat ctccagtatt gaggtgattg gcaaccgtgc
     3961 agtggcggac ggcaaaacca aacagacgta taaagttacg gtgactgatg ccaataacaa
     4021 cctgctgaaa gatagcgaag tgacgctgac tgccagcccg gcgaagttag ttctggatcc
     4081 caatgggacg gcgacaacga atgagcaagg gcaggctatt ttcaccgcca ccacaactgt
     4141 tgcggcgaca tatacactca cggcgcaagt gagtcagaac aacggtcagg tatcaacgaa
     4201 aaccgccgaa tctaaattcg tcgcggatga taaaaacgcg gtgctcgctg catctccaga
     4261 gcgtgtagat tctctggtgg cggacgggaa gactactgca acactgacgg ttactctgat
     4321 gtcgggtgtc aaccccgtag gaggaaccat gtgggtcgac attgaggctc cggaaggggt
     4381 gacagaggcg gattatcagt tcctgccgtc gaaaaatgac catttcgcga gcgggaaaat
     4441 cacgcgtaca tttagtacca acaagccagg tacatacaca ttcacattca actctttgac
     4501 atatggaggg tatgaaatga aaccagtgac tgtgacaatt aacgccgttc ctgcagatac
     4561 tgaaggcgct gaggagaaat aaaaacgacg atgtaataaa taataatggc gttgacatgc
     4621 gcagcgccat tatttactga atacctaccc tggcaccgat tagacggtgg cagggtaggg
     4681 taaatcttat gtgatggagt tacaggctaa accgggcgaa cggggataaa aatttcgcaa
     4741 acaatgttat tgctatcaat ttcgacgaag ttttcattgt aataaaatcg ctcaatatca
     4801 ctgccgttca ttctggctaa ccccctggca ggtaattcca tcagatagag gtttcgcgcc
     4861 cattcagggt agtgctccca ctcaccttca tagcggaaag aggcatacag tcctcggtta
     4921 attgtccact ctttcgacat ctctgaatcg gtttgtttgc ataccgtacc ggcaaaagtt
     4981 tcaactgtga gcaaatcgac cgattttgtt gatggttcga acgtggagac gaaatagata
     5041 tccgtcttaa aactcttcat acatctggct aaatgatgac gtcgcagttt gacttcttca
     5101 tccggtgatt tattcgacac ttcaatatca tatttaaatg aattaccaaa aataggtgtc
     5161 tcaggaaagt tgactaatct acactccgtc ttttgctgtt gacgtattaa aaaagaaggg
     5221 aagatatttg cgagatccca ataatcacgg tggcggtatt cacggggaga gcagccgaat
     5281 aactttttaa attcgcggct gaatgactgc tgtgagtcaa aatgcaaaga gagtgcaata
     5341 tcaagcatag atttcgcgga gagccggaca agaatggcgg cacgacaaag ccttcgtttg
     5401 cgaatgtatt ctcccaaagg cacatgcatg aaatttcgga acagaagctg gatgttgcgt
     5461 ctgctgtagc cagatttctg tgcgatatct tcgattgaaa tagggtgttc gagattgcac
     5521 tcaatccact ccaggagctg ctgtagaatc ttctgcctga tcatcacatt ctccgttaat
     5581 tctttgtcat atcgcacatt ttcgttatgc cctgggccct aagtgtatca aaaacagcaa
     5641 gggcgaaggc aaaagcactg gtagcgtctg cttcgttgtc cccgaaaata gagaggtttc
     5701 agtcctacat tattaatgaa ttttttgcat aagtgatatc aaaatccaca tactaatttg
     5761 aggttacgtt ttaacgtaga ctcattgttc atgcctaatg gagggactga cagtggaatt
     5821 ttccgtatta agtaacaatc tgaaaatgcc gatgatggga tttggtgttt ttcaggttac
     5881 cgataaaaac gtgtgcaaac agtcagtgtt gaacgctatc cgcacgggtt atcgactcat
     5941 cgacactgca gcggtatacg gtaatgaaga tgccgtcggt gaagccgttc gtgaagctat
     6001 atctgaaggt ttatgtaccc gagaagagtt atttataacg tctaaattgt gggtgcagga
     6061 tatgttgaat caggatattg cagcagcagg tattgaagca tcattaaaaa aatcgggact
     6121 agaatacttc gatctctatt tattgcacca ggccatgcgc gattatttta gcgcgtggcg
     6181 tgcactcgaa gatgcctatg aagagggcaa attaaaagcg attggggttt ccaatttcta
     6241 ccctcatgtt ctggcgaact tttgtgaaac ggtaagagtt aaaccgatgg tcaaccaggt
     6301 cgagttgcat ccctattttg cccaaccaga ggcgctggca accatgaagt attataacgt
     6361 gcagcctgaa gcatgggctc cgttaggtgg tggacgacat aaaccctttg aaaataatct
     6421 gcttcagagt attgcagatg cccatcaaaa atcgatttct caggtcattc tgcgttggaa
     6481 tattcaacgg ggagtggtcg ttattccgaa atcgacacat caacagcgta tcgaagaaaa
     6541 ttttgctatc tgggatttct cactgacaga gaaagaaatg gcgcaaatta gttcgcttga
     6601 tttgggttat gttggggaat cggtaaaaca ttttaatcct gagtttgttc gtggttgtct
     6661 tgctgtaaaa atacatgatt gatattaatc acttatttta cctgagacga caagaatctt
     6721 ttaacagggg agtgatattg atcttcactc tgtcatatct ccggtaatat ggcgtcaggc
     6781 ttcatcatca gaaagggggc ctgacgcctg aaaaagtgaa caacagacag tgttcggatt
     6841 atcaatattc agtttttaac gttgaactgg attcattact gcgttgttta agaatatccc
     6901 gcgccgaatc tgccattatc atgacagcac ctgccagcat cagagtatct ttcaatacca
     6961 gacgaccagc accggataaa taagggaaac catgatgagc gtcacccaat gcgggtaccc
     7021 atgcctccgg ggtggtgatt aaaaatgaga gtgttaccag cggcgtggta aatgccatca
     7081 gcccacccaa taaacctaac cagcgattga caggattagc caaaaccagc agagcaataa
     7141 tcacctccac gacgccaaga ccgttggaaa aaccataggt attattagcc gtttgccatg
     7201 ctcttgcttc tggtttgtat tcgccttcgt gagtcagatg ctgtttatag tcttccgggt
     7261 gttcataaaa gaacgacatt agtggactgt ttgcgacgaa tggtgtaatg ctgtctgcct
     7321 cgtaagggac aaactttaat aacccaatcc acataaaaat aatcgcaata ctcagacgaa
     7381 tcaatgtcag gccaatttta tctccccgac ttaacaggtg caggtatctt tccattgtaa
     7441 acatccttat ttatacaata ctgctgttgc ggttgaatta ttatcaattc gcatcgatgt
     7501 aactttccag gatgaagcat tatattttct ggccatatca gcaatggcat tctcaagaac
     7561 tgtcggtgat gctgatactg atgagacgct gatatgtttc agaattattt tatcatctgc
     7621 attggtggaa aatgccataa cgccagataa tagtgttgca aataaaacag attttttaaa
     7681 catataaaat accatcaatg aagtgattaa gaatttatag agtaacagag aattaataag
     7741 attcttttct ctgagacgcc aggatatttg ttctggcgtc tgattttgag tttatttgac
     7801 taatgaaaat agatcattga gtgattcgct catcgacgga tgagtaaata tctgatcgcg
     7861 taatatgcta taaggcagcc cggcatccat caccattttc actatattga tcatctcgtg
     7921 ggagtcaaca catagcagtg atgcccctaa tatacgttgg gttttattat caacaatcgc
     7981 ttttaatacc ccacgggtat cgttcatcac tctggcgcgc ggaattgcag ctacaggcaa
     8041 tgtcaccacc tgaatatcag caccactctc tctggcttgt tcttctgtca taccaaccct
     8101 ggacaggggc ggtgtcataa atacggaata aggcacattt ttccgatcat cagtactacg
     8161 tttgccttca cccagtaact catcacgtac aatgcggtaa tcatctagtg atatgtaagt
     8221 aaattgcagc ccgccggtaa catctcccat cgcccaaata ttgtctgcgg tggtatgtaa
     8281 tcgcttgtca acgacaattg ccccgcgctc gtttactgcg ataccggcat tttctggatg
     8341 taacgaagcg gtagctggtt gacgaccgga agctattaac agtgcatcca ccgccagttg
     8401 ggcgtgctcg ctatgcactt gcacttgatt ttcatggtga ctgattcgct ccacatgggc
     8461 attgaggatt atatcgacac cctgatcgcg taaaatcgtc gcgatattat cagcaatatc
     8521 ccgatcttcc cgaggcaaaa acagcgaagc tgcctctaaa atggttactt tgctgccaaa
     8581 attagcgaac atagaggcga actcaacgcc aatatatccg ccgcccaaaa tacctaaatg
     8641 cccaggcaat tcttttagat taagtaatcc ggtgctgtca tatacaccag gcgtggtggt
     8701 aattccagga attggcggaa ccacggcttg tgcaccggta ttaataaaaa ttttctcgcc
     8761 atgaatctcc agatttcccc caggccgatg aacacgcagg ctatgattat tgataaactc
     8821 cgcctggccg tcgatcacgt cgatattggg tatatccgca agattatgaa aattcttatt
     8881 acgtaaaaaa ttaaccactt catttttacg ctgtatggca cggacaaaat ctgtgtgctg
     8941 ctgtgcgtca tgaaccaatg ttttggttgg gatacagccg atattaatac aggtcccgcc
     9001 atacattgca tttgattgtt cgatgagagc cacacgccaa cctgcttttg ccagcgtgac
     9061 ggctaatgtt tttccagcct tgccaaaacc aataataact gcctgatatt tattcatgat
     9121 gatttcctgt cgaaacgcgt tcatttgaca tcatgactat agacgcaaaa gagcaaataa
     9181 catttcttca caacgcttga tattttgtct tatcgtctgg tattttcttt ttcttaatca
     9241 gaatcacaaa acaaaaatca tgattaacca tttgatgata ttagattaat tattgtctaa
     9301 gattgttggt gtttgtaatc aaaaaccact caggagtctg atatggatgc ccttagccgt
     9361 ttgttgatgc ttaacgctcc acaaggaacg atcgataaga attgcgtgtt aggaagtgac
     9421 tggcaacttc cacatggtgc cggggaattg tcggttattc gttggcatgc gttaacgcaa
     9481 ggagcggcga agctggaaat gccgacgggg gagattttta cattacgccc gggaaatgtg
     9541 gtcctgctac cacaaaattc agctcatcgc ctgagtcatg tcgataatga atcgacctgt
     9601 attgtctgtg gcactcttcg gctgcaacat tcggcgcgtt attttttaac atctttgccg
     9661 gaaacgctgt ttgtagcacc agtgaaccat agcgtggaat ataactggct gagggaggcg
     9721 atcccgtttt tacaacagga atccagatcg gcaatgccgg gagtggatgc tctgtgtagc
     9781 cagatctgcg ctacattctt taccctcgcg gtgcgtgagt ggattgcaca ggttaataca
     9841 gagaaaaaca ttctcagttt gcttctgcat ccacgtcttg gtgcggtaat acagcaaatg
     9901 ctggaaatgc caggacacgc ctggaccgtc gaatcgctgg ccagcatcgc ccacatgtcc
     9961 cgggcaagtt ttgcccagct tttccgtgat gtttccggaa ccacgccgct ggctgtatta
    10021 acaaagttgc gtctacaaat agcggcccag atgttttccc gggaaatgct ccctgttgtg
    10081 gtgatcgctg agtcagtagg ctatgccagt gaatcatctt ttcacaaggc gtttgtccgc
    10141 gagtttggtt gtactccggg agaatatcgg gaaagggtga gacagcttgc accctgaata
    10201 aaaccgccag aaatcagggt aaagataatc cgcattccgg gagttgtgag cctttcaact
    10261 atttctattt ccaacggtgg ttttgggctt tatatattt
//
LOCUS       DAZGQL010000147        10057 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.147, whole genome shotgun sequence.
ACCESSION   DAZGQL010000147 DAZGQL010000000
VERSION     DAZGQL010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10057
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.147"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            230..1387
                     /gene="acrE"
                     /locus_tag="ACHKIR_004658"
     CDS             230..1387
                     /gene="acrE"
                     /locus_tag="ACHKIR_004658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HFO9992303.1"
                     /translation="MTKHARFFLLPSFILISAALIAGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     SYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDESTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNNKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
     gene            1399..4503
                     /gene="acrF"
                     /locus_tag="ACHKIR_004659"
     CDS             1399..4503
                     /gene="acrF"
                     /locus_tag="ACHKIR_004659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
                     /protein_id="HFO9992304.1"
                     /translation="MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVS
                     VSANYPGADAQTVQDTVTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDI
                     AQVQVQNKLQLATPLLPQEVQQQGISVEKSSSSYLMVAGFVSDNPGTTQDDISDYVAS
                     NVKDTLSRLNGVGDVQLFGAQYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQ
                     LGGTPALPGQQLNASIIAQTRLKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYN
                     VIARINGKPAAGLGIKLATGANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQ
                     LSIHEVVKTLFEAIMLVFLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMMEDKLPPKEATEKSMSQIQGALVGIAMVLS
                     AVFIPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATLLKPVSAEHHENK
                     GGFFGWFNTTFDHSVNHYTNSVGKILGSTGRYLLIYALIVAGMVVLFLRLPSSFLPEE
                     DQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKNEKANVESVFTVNGFSFSGQAQNAG
                     MAFVSLKPWEERSGDENSAEAVIHRAKMELGKIRDGFVIPFNMPAIVELGTATGFDFE
                     LIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGLEDTAQFKLEVDQEKAQALGV
                     SLSDINQTISTALGGTYVNDFIDRGRVKKVYVQADAKFRMLPEDVDKLYVRSANGEMV
                     PFSAFTTAHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGDAMALMENLASQLPAGIGY
                     DWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVSVMLVVPLGIVGVLLA
                     ATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGVVEATLMAVRMRLR
                     PILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFFVPVFFVVIRR
                     CFKG"
     gene            4756..4977
                     /locus_tag="ACHKIR_004660"
     CDS             4756..4977
                     /locus_tag="ACHKIR_004660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992305.1"
                     /translation="MKRLIPVALLTALLAGCAHDSPCVPVYDDQGRLVHTNTCMKGTT
                     QDNWETAGAIAGGAAAVAGLTMGIIALSK"
     gene            5408..6433
                     /locus_tag="ACHKIR_004661"
     CDS             5408..6433
                     /locus_tag="ACHKIR_004661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO9992306.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAFNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            6501..7682
                     /gene="yhdX"
                     /locus_tag="ACHKIR_004662"
                     /pseudo
     CDS             6501..7682
                     /gene="yhdX"
                     /locus_tag="ACHKIR_004662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
     gene            7692..8795
                     /gene="yhdY"
                     /locus_tag="ACHKIR_004663"
     CDS             7692..8795
                     /gene="yhdY"
                     /locus_tag="ACHKIR_004663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFO9992307.1"
                     /translation="MTKVLLSHPPRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRTDCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAVWAVIYPLIVWWLMYGGFLGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKNQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            8803..9561
                     /gene="yhdZ"
                     /locus_tag="ACHKIR_004664"
     CDS             8803..9561
                     /gene="yhdZ"
                     /locus_tag="ACHKIR_004664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFO9992308.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(9624..9739)
                     /gene="rrf"
                     /locus_tag="ACHKIR_004665"
     rRNA            complement(9624..9739)
                     /gene="rrf"
                     /locus_tag="ACHKIR_004665"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(9779..9854)
                     /locus_tag="ACHKIR_004666"
     tRNA            complement(9779..9854)
                     /locus_tag="ACHKIR_004666"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9819..9821),aa:Thr,seq:ggt)
     gene            complement(9869..9984)
                     /gene="rrf"
                     /locus_tag="ACHKIR_004667"
     rRNA            complement(9869..9984)
                     /gene="rrf"
                     /locus_tag="ACHKIR_004667"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         2385 a         2410 c         2588 g         2674 t
ORIGIN      
        1 tttcaattta cgaggatttg attctacctc tttaaacccg cgtcaaaata aaacagtaga
       61 atattaatct ttttttgtgt ttatgtgcct tgagacgcca gtattcataa ctattcctta
      121 catcgacgaa tgataatttg taggatagcg aactgtattt ttctttctgc gagttaacgc
      181 gttgcctttt tgggtaaata acgcgctttt ggttttttga ggaatagtaa tgacgaaaca
      241 tgccaggttt ttcctcctgc cctcctttat tctgatctcc gcggctttaa tcgccggttg
      301 taacgataag ggagaagaga aagctcacgt cggtgaaccg caggttaccg ttcatattgt
      361 gaaaacggcc ccgttagaag ttaagactga attaccaggc cgcaccaatg cttaccgtat
      421 agccgaagtt cgcccacagg ttagcgggat cgtactgaat cgcaatttca ctgaaggcag
      481 cgatgtgcaa gcaggccagt ccctgtacca gatcgatccc gcgacctatc aggcaagtta
      541 tgacagcgcg aaaggcgaac tagcgaaaag tgaagccgcc gccgccatcg cgcatttgac
      601 ggtaaaacgt tacgttccgc tcgtgggtac gaaatacatc agtcagcagg agtacgacca
      661 ggccattgct gatgctcgcc aggccgatgc cgccgtgatt gccgcaaaag ctacagttga
      721 aagcgctcgc atcaatctcg cttataccaa agtgactgcg cctattagcg gacgtatcgg
      781 caaatcgact gtgaccgaag gcgctcttgt cactaatggg caaacgactg aactggcgac
      841 tgtccagcag ctcgatccta tctacgttga tgtgacccaa tccagcaacg attttatgag
      901 gctgaagcaa tccgtagagc aaggaaattt gcataaggaa aacgccacca gcaacgtaga
      961 gttggtcatg gagaacggtc aaacctatcc cctgaaaggt acgctgcaat tctccgatgt
     1021 gaccgttgat gaaagcaccg gctccataac cctacgtgct gtcttcccta acccgcaaca
     1081 tacgcttttg ccgggtatgt ttgtgcgcgc acggattgat gaaggcgtcc aacctgacgc
     1141 cattcttatc ccgcaacaag gcgttagccg cacaccacgt ggtgatgcaa ccgtgctgat
     1201 tgttaacaat aaaagtcagg ttgaagcgcg ccctgtcgtt gccagccagg cgattggcga
     1261 taaatggttg attagcgaag ggctgaaatc tggcgatcaa gtcattgtca gcggcctaca
     1321 aaaagcgcgt ccgggagagc aggttaaagc cactaccgat acccccgcag atactgcatc
     1381 gaagtaaggc aatctgacat ggcaaacttt tttattcgac gaccgatatt tgcatgggtg
     1441 ctggccatta ttctgatgat ggcgggcgca ctggcgatcc tacaattgcc cgtcgctcag
     1501 tatccaacca ttgcaccgcc tgcggtttct gtttcagcaa actatccggg cgctgatgcg
     1561 cagaccgtgc aggatacggt gacgcaggtt atcgaacaga atatgaacgg tatcgataac
     1621 ctgatgtata tgtcctccac cagcgattcc gccggtagcg tgacaattac ccttaccttc
     1681 cagtccggga ccgatcctga tatcgcgcaa gtgcaggtgc agaacaaact ccagctcgcc
     1741 acgccgttgc tgccgcagga agtacagcag caggggatca gtgttgaaaa gtccagtagc
     1801 agctatttga tggtggcggg ctttgtctct gataacccag gcaccacaca ggacgatatc
     1861 tcggactatg tggcctctaa cgttaaagat acgcttagcc gtctgaatgg cgtcggtgac
     1921 gtacagcttt tcggcgcaca gtatgcgatg cgtatctggc tggacgccga tctgctaaac
     1981 aaatataaac tgacaccggt tgatgtgatt aaccagttga aggtacagaa cgatcagatc
     2041 gctgccggac agttgggcgg aacgccagcg ttaccagggc aacagttgaa cgcctcgatt
     2101 attgctcaga cgcggttaaa aaatccggaa gaattcggca aagtgaccct gcgcgtaaac
     2161 agtgacggct cggtggtacg cctgaaagat gtcgcacggg ttgaacttgg cggtgaaaac
     2221 tacaacgtta tcgctcgtat caacggaaaa ccggcggcgg gcctggggat taagctggca
     2281 accggcgcaa atgctctcga taccgcgaaa gccattaagg caaaactggc ggaattacag
     2341 ccattcttcc cgcagggaat gaaggttctc tacccttatg acaccacacc attcgtccag
     2401 ctttctattc acgaagtggt aaaaacgctg ttcgaagcca ttatgctggt gttcctggtg
     2461 atgtatctgt tcttgcagaa tatgcgagca acgctgatcc ccaccattgc ggtacccgtg
     2521 gtgttgttag ggacgtttgc catcctcgcc gcttttggtt actccatcaa cacactaacg
     2581 atgttcggga tggtgcttgc catcgggctg ctcgtcgatg atgcgatagt ggtggtggag
     2641 aacgtcgagc gcgtgatgat ggaggataag ctcccgccaa aagaagcgac ggaaaaatca
     2701 atgtcgcaaa tccagggcgc actggtgggt atcgcaatgg tgctgtcagc ggtatttatt
     2761 ccgatggcat tcttcggcgg ttctactggg gcaatttatc gtcagttctc tatcaccatc
     2821 gtttcggcaa tggcgctttc tgttctggtg gcattgattc ttacccctgc gttatgtgca
     2881 acgctgctta aacccgtctc tgctgagcat cacgaaaata agggcggttt cttcggctgg
     2941 tttaatacca ccttcgatca tagcgttaac cactacacca acagcgtcgg caaaatcctc
     3001 ggttccacag gacgatattt actgatctat gcactaattg ttgcaggaat ggtggtgttg
     3061 tttttacgtc ttccgtcttc cttcttacct gaagaggatc agggtgtctt tctgactatg
     3121 attcagttac ccgctggcgc gacgcaagag cggacgcaaa aagtactgga tcaagtgacg
     3181 gattactatc tgaagaacga gaaagcgaac gttgaaagtg tctttacagt taacggcttt
     3241 agcttcagcg gccaggcaca aaacgccggt atggctttcg tcagtctgaa accgtgggaa
     3301 gagcgtagtg gtgacgaaaa cagtgcggaa gcggtaatcc atcgtgccaa aatggaattg
     3361 ggcaagatcc gcgacggttt tgtcattcca ttcaatatgc cagccattgt tgaactgggc
     3421 acggcaacgg gtttcgactt tgagttaatt gatcaggctg ggctgggtca tgatgccctg
     3481 acccaggccc gtaaccagtt gcttggcatg gcggcgcaac atcctgccag cttagtcagc
     3541 gtgcgtccta atggtctgga agacaccgcg cagtttaaac tggaagttga ccaagaaaag
     3601 gcgcaggcat taggtgtttc actttctgac atcaatcaga ccatttcaac agcgttgggt
     3661 gggacttacg ttaacgactt cattgaccgt ggtcgcgtaa aaaaggtgta tgttcaggcg
     3721 gatgccaaat ttcgtatgct gccagaagat gtcgataaac tttatgtccg tagcgccaat
     3781 ggcgaaatgg tgccattctc tgcctttacc actgcacatt gggtgtatgg ttctccgcga
     3841 ctggagcgct acaacggtct gccgtcaatg gagattcagg gggaagccgc gccaggaacc
     3901 agttccggcg atgccatggc gttgatggaa aaccttgcat cacaattacc agctggtatt
     3961 ggttatgact ggacgggtat gtcatatcag gaacgcttat cgggaaacca ggctcccgct
     4021 ctggtagcaa tttcctttgt ggttgttttc ctgtgccttg ctgcactcta tgaaagctgg
     4081 tcaattcctg tctcggttat gttggtcgtg ccgttaggga ttgtcggcgt gctactggcg
     4141 gcgacactct ttaatcaaaa aaatgacgtc tactttatgg tgggcttgct aacgacaatt
     4201 ggcttgtcgg ctaaaaacgc tatattgatt gttgagttcg ctaaagatct catggagaaa
     4261 gagggtaaag gtgttgttga agcgacacta atggcagtac gtatgcgtct gcgtcctatc
     4321 ctgatgacct ctctcgcctt tattctcggc gtattaccgc tagctatcag taacggtgcc
     4381 ggcagtggcg cgcagaacgc agtgggtatc ggggtaatgg gcgggatggt ttctgcaacg
     4441 ttgctggcaa tcttcttcgt gccggtgttc tttgtggtga tccgccgttg ctttaaagga
     4501 taaatcagaa acataaaggc actttcgggt gcctttatta tttccagtga aacccataaa
     4561 aattaataag atattcctct actcactctt taaaaacttt ctatagttcc cgctcccttc
     4621 actattttta caattcacat aatttgtcat tattcattcc gcaagattta taacctccag
     4681 tgataaaatg gcattcagct cgttaataag agagttaact tattaagcgt tagcgtttat
     4741 tactgaggta acaccatgaa aagattaatt cctgtggcat tgctcaccgc attgctggcg
     4801 ggctgtgcgc acgattcgcc ctgcgttccg gtttatgacg atcagggtcg tctggttcac
     4861 accaatacct gtatgaaagg cacgactcag gataactggg aaacggctgg ggctattgcc
     4921 ggcggggcag cagctgttgc tggcctgacg atggggatca tcgctctgtc gaaataacta
     4981 aacagggcgc ggagctccgc gctcttttta acgatacagt atctttccat aagataaaat
     5041 attgctatat tctgctgggt aattccctgc tgatttagca tgttatatca cgttatttca
     5101 ctgctttcga aataacgcat caatttaact ttcacacatc ccaccagaaa tatactcacg
     5161 ctaacaatca ttattctgct tagtaaaaaa cgcgtaaata ttttgcgcca aaatgtggcg
     5221 catgtttcat tttcgcacca ttgcggggcg cagtttttat tatttcctgt ctacactcag
     5281 cttattgcga cgcgaaatcg tgcgcaaaag ctggcactac ttttgcttat aagaggatgg
     5341 ccacagacag gtaaaagacg tttcccaaac gtcctataac gataattttc gcctcacagg
     5401 aagcattatg aaaaagatga tgatagccac actggctgcc gccagcgtgc tgcttgccgt
     5461 tgcaaatcag gcgcatgctg gcgcgacgct tgatgccgtt cagaaaaaag gttttgtgca
     5521 atgcgggatc agtgatggat tacctgggtt ctcttatgcc gatgctgacg gtaagttttc
     5581 aggtattgat gtagatgttt gtcgtggtgt tgccgctgct gtattcggtg acgatacgaa
     5641 agtgaaatat accccgctca ctgcaaaaga acgcttcacc gctttacagt caggggaggt
     5701 ggatttgctc tcccgtaata cgacctggac ttcatctcgc gatgccggga tgggaatggc
     5761 atttactggc gtcacttatt acgacggcat tggcttcctg acgcacgata aagcggggct
     5821 aaaaagcgcg aaagaactgg atggcgctac cgtctgtatt caggcgggta ctgataccga
     5881 actcaacgtt gccgattact ttaaggcaaa caatatgaag tacacaccag tgactttcga
     5941 tcgctctgac gaatcagcga aggcactgga atctggtcgc tgcgatacgc tggcctcgga
     6001 tcaatcacaa ctgtatgccc tgcgcatcaa attaagcaac ccggctgaat ggattgtctt
     6061 accggaagtt atctctaaag aaccacttgg gccggtagtt cgtcgtggcg atgatgaatg
     6121 gttctcgatt gtacgctgga cgcttttcgc catgctgaat gctgaagaga tgggcatcaa
     6181 ttcccagaac gtcgatgaaa aagcggctaa tccagcaaca cctgatatgg cacatctgct
     6241 gggtaaagaa ggcgattacg gcaaggatct gaagctggat aataaatggg cctttaacat
     6301 catcaaacag gtgggtaact actcggaaat tttcgagcgt aacgtaggtt cagaaagccc
     6361 gctgaaaatt aaacgtgggc aaaataatct ctggaataac ggcggtattc agtacgcgcc
     6421 gcccgtgcgt taagtcggtg tgaggtaacg agcgtcgcta cggcggcgct ctcttcagcg
     6481 ttatgctttc cgaggtttct atgtctcatc gccgctcaac cgttaaaggc tcactctcct
     6541 ttgccaaccc tacggttcgc gcctggttat tccagatctt tgccgttgtt gctgttgtcg
     6601 gaattgttgg ttggctattt cacaacactg tgacgaatct cagtaatcgt ggcattactt
     6661 caggttttgc ctttctggat cgcggcgctg gcttcggtat tgtccagcat ttgatcgatt
     6721 accagcaggg tgatacctac ggacgcgttt tcattgttgg cttactcaat acgctactgg
     6781 tttctgcatt gtgtattgta ttcgcttctg ttctgggctt ctttatcggt ctggcgcgac
     6841 tttcggataa ctggctgcta cgaaagcttt ccacaattta tattgagatc ttccgtaata
     6901 ttccgccgct gctgcaaatc ttcttctgat actttgccgt gttgcgcaat ttgcccggac
     6961 cacgccaggc agtgagtgcg tttgatctgg cctttttgag caatcgtggg ctttatattc
     7021 catctccgca gctgggagac ggatttattg cgtttatcgt gactgttgtt attgctatag
     7081 ccatttctgt tggactattc cgttttaata aaacgtacca gataaagacc ggacaattgc
     7141 gccgaacctg gccggtcgcc gttatgttga tcataggttt gcctttactg gcgcaatggc
     7201 tttttggcgc agcactacac tgggatgttc cagccctacg aggctttaac ttccgtggcg
     7261 ggatggtttt aattcctgaa ctggcagcct taacgctggc gctttcggtt tatacctctg
     7321 catttatcgc cgagattatc cgcgctggga tccaggcagt gccttatggt caacatgaag
     7381 cggctcggtc actgggatta cccaatccgg ttacgctacg ccaggtcatt attccccagg
     7441 cactgcgagt gattattccg ccgttaacca gccagtatct caacatcgtc aaaaactcct
     7501 ctcttgccgc cgctattggc tatccggata tggtttcgct gtttgctggc accgtgctga
     7561 accagacggg gcaagccatc gagacgatag ccatgaccat gtcggtctat ctgattatca
     7621 gcctgactat ctcgctgctg atgaatatct ataaccgccg catcgcgatc gttgaacgct
     7681 aaggagtcat gatgacaaaa gtattgctgt ctcatccccc gcgcccggcg agccataact
     7741 caagccgcgc gatggtgtgg gtgcgaaaaa atctgttctc cagctggagc aatagcctgc
     7801 tgactattgg ctgcatatgg ttgatgtggg aactgattcc accgttgctg aactgggcat
     7861 ttttgcaggc taactgggtt ggctcaacgc gtaccgactg cacaaaagcc ggtgcctgtt
     7921 gggtcttcat ccacgaacga tttggtcagt ttatgtatgg gctttaccca cacgaccaac
     7981 gctggcgaat taacctcgca ttactgattg ggcttgtgtc gatcgcacca atgttctgga
     8041 aaatactccc gcatcgcggt cgctatattg cggtatgggc ggtgatttac ccactgattg
     8101 tctggtggct gatgtatggc gggtttcttg gtcttgagcg ggttgaaacc cggcaatggg
     8161 gcgggctgac gctaacttta attattgcat cagttgggat tgcgggggcg ctgccgtggg
     8221 ggatcttact ggcgttaggt cgccgctccc atatgccgat tgtgcgtatc ttatcggtca
     8281 tttttatcga gttctggcgc ggtgtaccgc tgataaccgt tctgtttatg tcttcggtca
     8341 tgctgccgtt gtttatggca gaaggcacca gtatcgacaa attgatccgc gcgctggttg
     8401 gcgtgatcct gtttcagtca gcatatgttg cggaagtcgt gcgaggcgga ttacaggcgc
     8461 tgcctaaagg gcaatatgaa gcggcagagt cgctggcgtt gggttactgg aaaaaccagg
     8521 ggctggttat tctgccacag gcgttgaagc tggtgattcc tgggctggta aataccatca
     8581 tcgcactctt caaagatacc agcctggtga tcatcatcgg gttgttcgat cttttcagta
     8641 gcgttcagca ggcaaccgtt gatcccgcct ggttgggtat gtcgacggaa gggtatgttt
     8701 tcgccgcact gatctactgg atcttctgtt tcagcatgtc acgttatagc caacatctgg
     8761 aaaaacgttt taacaccggg cgtacaccgc attgaggaca ctatgagcca aattttactg
     8821 caacctgcta acgcgatgat tacgctggaa aacgtcaata aatggtatgg gcaattccat
     8881 gttttgaaaa atataaattt aaccgtgcaa ccgggagaac ggatcgttct gtgtggccct
     8941 tctggttccg gtaaatcgac aaccattcgt tgtattaatc atctggaaga acaccaacag
     9001 ggacggatcg tggtagatgg catcgagctt aacgaagata ttcgcaatat tgagcgcgtc
     9061 aggcaggaag tgggaatggt ctttcagcat ttcaatctct tccctcatct gaccgttcta
     9121 cagaactgta ccctggcacc gatttgggta cgcaagatgc caaagaaaga ggcagaagct
     9181 ctggcgatgc attaccttga gcgggtgaga attgccgaac atgcgcataa gtttcccgga
     9241 cagatttcag gtggtcagca gcaacgcgtt gccattgcgc gttcgctgtg tatgaagccg
     9301 aaaattatgt tatttgatga gccaacgtcg gcgctcgatc ctgagatggt gaaagaggtg
     9361 ctggatacga tgattgggct ggcgcagtcg ggtatgacaa tgctgtgtgt aacacatgag
     9421 atggggtttg cacgaaccgt cgctgacagg gtgattttta tggatcgtgg ggagatagtg
     9481 gaacaagctg caccggatga gttttttgcg catcctaaat cagagcgtac gagggcattt
     9541 ttatcgcagg tgatccatta attgaatgtt agttcgaaaa gcaaaaaggc catcctttcg
     9601 gatggccttt cgcttgattt gatgtctggc agttccctac tctcgcatgg ggagacccca
     9661 cactaccatc ggcgctacgg cgtttcactt ctgagttcgg catggggtca ggtgggacca
     9721 ccgcgctact gccgccagac aaattctttt ctaatctgcc gaactttaac ctaaaaagtg
     9781 gtgctgatac ccagagtcga actggggacc tcacccttac caagggtgcg ctctaccaac
     9841 tgagccatat cagcacgcta aatttgatgc ctggcagttc cctactctcg catggggaga
     9901 ccccacacta ccatcggcgc tacggcgttt cacttctgag ttcggcatgg ggtcaggtgg
     9961 gaccaccgcg ctaaggccgc caggcaaatt ctgttttatc aacacgtctc gttatgacct
    10021 gtcgatttaa tctgtatcag gctgaaaatc ttctctc
//
LOCUS       DAZGQL010000148         9917 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.148, whole genome shotgun sequence.
ACCESSION   DAZGQL010000148 DAZGQL010000000
VERSION     DAZGQL010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9917
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.148"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            41..1285
                     /locus_tag="ACHKIR_004668"
     CDS             41..1285
                     /locus_tag="ACHKIR_004668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA C-terminal domain-containing protein"
                     /protein_id="HFO9992309.1"
                     /translation="MSKKFTKTLLSSSLVVGLLSVSSFTSASEYSYTALDIVGRHGTT
                     YTIVYNSDSDTAYVKWKTVPAGAEGIESGSIVQGLSKINRDGTVEVTTKDGTSTYRVY
                     DREFHDLMNSLVKQKREGSLGKNEGTKTELVSDADIRNLFGRIAAISEMETNVGLDEY
                     GRVATSAESIKERVSLQSYLATEAANSAIGLDKNGKLTLNETDIVTPLRINDGVVTIA
                     KDVEEQKEGLAIQKKGLSELSSRTDIIDAATAANTNKIDSRTTVGVNADGTLTSADGA
                     TKTIAVNDGLVALSGRTDRIDATVGSVDRRVTKNTQAIQSNTRQLQEHNARLNSQQRQ
                     IRENHEEMKRAAAQSAALAGLFQPYSVGKFNATAALGGYSDKQAVAVGVGYRFNEQTA
                     AKAGVAASDGDVSYNVGVNFEF"
     gene            1372..1845
                     /locus_tag="ACHKIR_004669"
     CDS             1372..1845
                     /locus_tag="ACHKIR_004669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992310.1"
                     /translation="MKVILATRNRYLEYGLQQMLEGYRIILAREFFTPENRKSVPAHD
                     ESWVIICDALLGRLMCCMFQGRRYLQIDAEDVTGRLETYRKIRNGEWVHNTYARPLTM
                     SEMVVMFGYVYRESKPCHLAREMGINTKTVNTFLYMGLGKNGLKYRSVKHLVGRA"
     gene            2216..6208
                     /locus_tag="ACHKIR_004670"
     CDS             2216..6208
                     /locus_tag="ACHKIR_004670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S6 family peptidase"
                     /protein_id="HFO9992311.1"
                     /translation="MNKIYSLRVNDRKELVAVPETVGGRKKSSGIPGAGMLSRLLLAS
                     GAVAGVLFSSPALASVVGNTLPWQTYRDFAENKGAFHAGATNIPLYGKNGAVSGRLDK
                     APMMDFSVVDQILGVATLISPQYVAGVKHNGSYNTVRFGYADDTTYRLVDRNEHWRDF
                     HTPRLNKLVTEVAPVSVTDAGTGKGVYQNRSRYPVFYRMGSGTQYTGAASGALTRIAD
                     AYVWRTGGTVGSPVISDWSLVSNPGNLYQGVNGPLASYGTSGDSGSPLFAWDAVKKQW
                     VFVAVLHGYAGENGKTNWFTVIPAGDVNNTIKQDSSGTVVPAVAGGDIVWNYSKGSGE
                     GTLSQDGKVWKMNGFRGGSLNDGKDITFGGKGTVVLKDDVVQGAGSLTFNGDYTVRPE
                     GNQTWVGGGIIVNDGHRVDWMVNGLAGDALHKIGKGTLVVAGSGENPGTLNTGDGTVI
                     LAQKADAAGRVRAFSEVSIVSGRPVVVLQDSHQIEGDRIRWGYRGGTLDINGNDIAFN
                     RLAAADEGAVLTSRARPATVRLDFSQSGQKAVMWHGHFTGNLSVLNNTSSAVDFIMDG
                     GADISGSFTQQGGGLYIQGHPVVHAVSSEAVATALRKQGDNSVLTQPVSFAQKDWESR
                     TFSIGQLKLKGAAFSLSRNATLTGDIDADNATMVLGSDSLYLDMKDGTGSSSAPVKGT
                     SAVGGASGSTTFRGNVNMRHSALTVRDHFTGSITASDSRIAVSSENVRLEGNSRLTSS
                     ALTVSDGGRLHVKGGLETDGGVTLDRGTLLVDGGSVRNDVYERLLAWSEERGGLNGSG
                     EYDFMTGAAGLLRSYVRGSAGNVNLQNAAWMMTGNSSVKHLESSGSALYFSRPGGEFH
                     TLTAGSMDISDSVLVMRTDLNHSDQLRVTESLRGKNNLLLVDFTERSDGQKALNIPLV
                     TAPAGTGADVFSVKTRDTGFSHITPVVRAEQGTGGTAWQLNVVQPETAAEPVATRQDA
                     ETPNPVEAVSPLPSPVSAPSSAPEASVTDVLTGENAGESGEYRDETRWLLTGYRSTVN
                     SSAVRDAGMLMSMGHRNFINEVNNLNKRMGDLRDINGEAGAWARIMSGTGSAGGGFSD
                     NYTHVQVGADKKHELDGLDLFTGVTMTYTDSNAGSDTFSGKTKSVGAGLYASALFDSG
                     AYIDLIGKYVHHDNEYTASFAGLGTKDYSSHSWYAGAEVGYRYHVTEDAWIEPQAELV
                     YGAVSGKQFSWKDQGMNLTMKDKDFNPLIGRTGVDVGKSFSGKDWKVTARAGLGYQFD
                     LLANGETVLRDASGEKRIKGEKDGRMLMSVGLNAEVRDNIRFGLEFEKSAFGKYNVDN
                     AVNASFRYSF"
     gene            complement(6776..9448)
                     /gene="rcsD"
                     /locus_tag="ACHKIR_004671"
     CDS             complement(6776..9448)
                     /gene="rcsD"
                     /locus_tag="ACHKIR_004671"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HFO9992312.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTTPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGDNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPNTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPEGKQLTNQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
BASE COUNT         2477 a         2126 c         2830 g         2484 t
ORIGIN      
        1 tggctgattt gtatacagat aatttaacag gatatccata atgagtaaaa aatttacaaa
       61 gacacttctg tcatcttctc tggttgtagg tctgttgagt gttagttctt ttacaagtgc
      121 aagtgaatat agctacactg ccttggatat tgtcggtaga cacggaacta cttacaccat
      181 tgtttataat agtgattctg acaccgctta tgttaaatgg aaaacagtac ctgccggtgc
      241 tgaagggatt gaaagtggaa gtattgtaca aggattatca aaaattaata gagatgggac
      301 tgtagaagtt actaccaagg atggcactag tacatatcgt gtgtatgacc gggagtttca
      361 tgacctaatg aattcactgg tcaagcagaa aagagaaggt tctcttggaa agaacgaggg
      421 tactaaaaca gagcttgtat ctgatgctga tattcggaat ctttttggca gaattgctgc
      481 gatctctgaa atggaaacta atgttggtct ggatgaatat gggcgagtgg caactagtgc
      541 agagagtatt aaagaacgcg tgtctttgca gagttatctg gctacagagg ctgcaaatag
      601 cgcaattggc ctagataaaa acggtaagct aactctgaat gagacagata ttgtaacgcc
      661 gttacgtatc aatgacggcg ttgtcactat agcgaaagat gtggaagaac agaaagaagg
      721 gctggcgatc cagaagaaag gactgtctga gctttcatcc cgcacagata tcattgatgc
      781 agcaacggct gcgaatacga ataaaattga ttcccgaacc accgttggtg tgaatgcgga
      841 cggaaccttg accagcgctg atggcgctac aaaaacgatt gctgttaatg acggtctcgt
      901 tgctctgtcc ggcagaacag accgtattga tgctactgtt ggtagcgttg accgccgggt
      961 tactaaaaat acacaggcta ttcagtccaa tactcgccag ttgcaggaac ataatgcacg
     1021 cctgaacagc cagcagcgtc aaattcgtga gaatcacgaa gagatgaagc gtgcagcagc
     1081 acagagtgca gcgctggcgg ggcttttcca gccgtacagt gtggggaaat tcaacgccac
     1141 tgctgccctg ggggggtaca gtgataaaca ggctgttgcc gtgggtgttg gttaccgttt
     1201 caatgagcag acagcagcga aagcaggtgt ggcagcgagt gatggcgatg tgtcctacaa
     1261 cgtaggtgtg aactttgagt tttaattgct gacagtgaca tgaagcatga cggcaggcgt
     1321 ggagttttgc ctgccgtttt ttctcatgtg agcatgaaac aggtgacagc gatgaaagtg
     1381 attctggcga cccgcaaccg ttatctggag tatggcctgc agcagatgct tgaggggtac
     1441 aggataatac tggcgaggga gttttttaca ccagagaaca ggaagagtgt accggcacat
     1501 gatgagtcat gggtgataat ctgtgatgcc ctccttggca ggctgatgtg ctgcatgttt
     1561 cagggacgcc gttatcttca gattgatgcg gaagatgtta cggggcgcct ggagacgtac
     1621 aggaaaatcc ggaatggtga gtgggtacat aatacgtatg cgcgacctct gacgatgtcc
     1681 gagatggtgg tgatgtttgg atacgtgtac cgggagtcaa agccctgcca tcttgcccgt
     1741 gaaatgggga taaacacgaa gacggtgaac acctttctgt atatgggact tggtaaaaat
     1801 ggtcttaagt acaggagtgt gaagcacctg gttggacgtg cgtagccgga atgcatattc
     1861 gtgagtcggg taatgagagt ggaacggtgg cagtgagaat ggaacagaaa aaaatcagat
     1921 gcggaaagcg gaaatacaca gcgggtgcga tgtgcttcat gctgtttgta tggctggggg
     1981 gtgtggtgac attctgtgtg ctggccgctc tgataatgag gtgaaaacgg aagagagttt
     2041 acttccttaa ttcgaacttt ctgataactg agaacttgtt aaatgcctgt gggtttatgt
     2101 atttaaatca attggttaat ggtgatttta tctttatcag tcaaagttcg ttttagtatg
     2161 atgattgtgc ccgctgttta ttgttgtccg ggagaaaata cagggaatcc aggggatgaa
     2221 caaaatatac tcactcagag ttaatgacag aaaagaactg gttgctgtac cggaaacggt
     2281 ggggggcagg aaaaagtcgt ccggcattcc cggtgcgggg atgctttccc gtcttcttct
     2341 ggcctcagga gccgttgccg gcgttctgtt ttcttctcct gcgctggcat ccgttgtcgg
     2401 gaatactctg ccatggcaga cctacaggga ttttgctgaa aacaaagggg cgtttcatgc
     2461 cggggccaca aatattcctc tttatggtaa gaatggggct gtcagtgggc ggctggacaa
     2521 ggctccgatg atggatttca gtgttgtgga ccagatactg ggggtggcaa cactgatttc
     2581 tccgcaatat gtggcggggg ttaagcataa cggcagctac aatacggtgc gttttggtta
     2641 tgcggacgat acgacatatc gccttgttga ccgtaatgag cactggcgtg attttcatac
     2701 tccccggctg aacaaactgg tgacagaagt tgccccggtg agcgtcacgg atgccggaac
     2761 cggcaagggg gtataccaga accgcagtcg ttatccggtg ttttatcgta tggggagtgg
     2821 aacgcagtac accggagctg cttccggtgc gctgaccaga atagcggatg cttacgtctg
     2881 gaggactggc gggacggtgg gaagtccggt catttctgac tggagtctgg tgtcaaaccc
     2941 gggaaatctt taccagggtg ttaatggtcc gctggcgtct tacgggacat caggagacag
     3001 tggctcaccg ctttttgcct gggatgcggt gaagaagcag tgggtatttg ttgctgtact
     3061 tcatggttat gccggtgaga acgggaagac gaactggttt acggtgattc ctgcagggga
     3121 tgtgaataac acgataaagc aggacagttc aggcaccgtc gtgccggcag ttgccggtgg
     3181 tgacattgtg tggaattaca gtaagggaag cggagaggga accctttctc aggatgggaa
     3241 ggtatggaag atgaacggct tcaggggagg gagtctgaat gacgggaaag atatcacctt
     3301 tgggggtaag ggcacggttg ttctgaagga tgatgttgtc cagggggcgg gttcgctcac
     3361 gtttaatggt gattatacgg tcagaccgga agggaatcag acctgggtgg gcgggggaat
     3421 catcgtgaac gacgggcacc gtgttgactg gatggtgaac ggacttgcgg gagacgcact
     3481 tcataaaatt ggtaagggaa cactggtggt ggccggcagt ggtgaaaatc ctggcaccct
     3541 gaatacgggg gacggaacgg tcattctggc acagaaggct gatgctgcag gcagggtccg
     3601 ggcattcagt gaagtcagta ttgtcagcgg acgaccagtt gtggtgctgc aggacagtca
     3661 tcagattgaa ggggaccgca ttcgctgggg ataccgggga ggtacgcttg atatcaacgg
     3721 aaatgacata gccttcaacc gcctggctgc ggctgatgag ggggcagtac tcacaagccg
     3781 ggcgcgtcct gccacagtcc gtctggattt cagccagtca gggcagaagg ccgtgatgtg
     3841 gcatggtcat tttaccggta acctcagcgt cctgaacaac acgtcatctg cggtggattt
     3901 tatcatggat ggcggagcgg atatatccgg cagttttaca cagcagggcg ggggactgta
     3961 tatccagggg catccggttg ttcatgctgt cagcagtgag gcggttgcga ctgcattgcg
     4021 aaagcagggt gataattctg ttctgacgca gccggtatca tttgctcaga aggactggga
     4081 gagcagaacg ttcagcatcg gtcaactgaa actgaagggg gcggcattca gcctttcccg
     4141 caatgcgaca ctgacgggag atattgatgc tgataatgcc accatggtgc tgggaagtga
     4201 ctcgctatat ctggatatga aggacggcac ggggagcagc agtgcgccgg ttaaggggac
     4261 gtctgccgtg ggcggtgcat ccggtagcac tacatttcgc gggaatgtga atatgcgtca
     4321 ttctgccctg acggtccgcg accattttac tggcagtata acggcatcgg acagccggat
     4381 agctgtgagt tcagagaatg tgcgtctgga agggaattcc cgcctgacct cttctgcact
     4441 tactgtcagt gacggcgggc gtttacatgt gaagggggga ctggagacag atggtggtgt
     4501 gacactggac agaggaaccc ttttggtgga cgggggaagt gtcaggaatg atgtatatga
     4561 acgtctgctg gcctggtcag aggagcgtgg cgggctgaac ggaagcggtg agtatgattt
     4621 tatgacgggg gcagctggtc tcctgcggag ttatgtcaga gggagtgcag gtaatgtgaa
     4681 tctgcagaat gcggcctgga tgatgacggg aaactcatcc gtgaagcatc ttgagtcatc
     4741 gggaagtgct ctgtatttca gcaggccggg cggggagttc cataccctga cggcaggcag
     4801 tatggatatc agcgacagtg ttctggtgat gcggacggac cttaaccact cagaccagct
     4861 tcgggtgaca gaatcgctgc ggggtaagaa taacctgttg ttggttgatt ttactgagcg
     4921 ttctgacggg cagaaggcgc tgaatatccc tctggtgacg gcgccggccg gtaccggtgc
     4981 ggatgtgttc agtgtgaaaa cgcgtgatac cggcttcagt catatcacac ctgttgtcag
     5041 ggctgaacag gggacgggcg gcacggcctg gcagctgaat gttgtgcagc cggaaactgc
     5101 cgcagagcct gtggcgacgc ggcaggatgc agagacgccg aatcctgtgg aagctgtatc
     5161 accgctccct tcaccggttt ctgctccgtc atcggctccg gaggcgtcag tgacggatgt
     5221 actgaccgga gagaatgcgg gggaatcagg tgagtaccgt gatgagaccc ggtggctgct
     5281 gacaggatac cggagtacgg ttaatagctc cgcggtcagg gatgcgggga tgctgatgtc
     5341 aatggggcac cgtaacttca ttaatgaggt caacaacctg aacaaacgta tgggtgacct
     5401 gcgggacatc aacggcgagg ccggtgcatg ggcccgtatc atgagcggaa ccggctctgc
     5461 cggcggtggc ttcagtgaca actacacaca cgttcaggtg ggggcggata aaaaacacga
     5521 actggacggt ctggacctct tcaccggggt gaccatgaca tataccgaca gcaacgccgg
     5581 cagcgacacc ttcagtggta aaacgaagtc agtgggtgcg ggtctgtatg cttcagccct
     5641 gtttgattcc ggggcctata tcgacctgat tggtaagtat gttcaccatg ataatgagta
     5701 tacggcgtcc tttgccggac tcggcacaaa agactacagc tctcattcct ggtatgccgg
     5761 tgctgaagtg ggttaccgct atcatgtcac agaggatgcc tggattgagc cacaggcgga
     5821 actggtctat ggtgctgtct ccgggaaaca gttcagctgg aaggaccagg gaatgaacct
     5881 caccatgaag gataaggact ttaatccgct gattggccgt accggtgtgg atgtgggtaa
     5941 atctttctcc ggtaaggact ggaaagtgac ggcacgtgct ggtcttggct atcagtttga
     6001 cctgctggcg aacggagaaa cggtactgcg tgatgcatcc ggagagaagc gtattaaggg
     6061 cgagaaagac ggccgtatgc tgatgtctgt gggtctgaac gcagaggtcc gtgacaacat
     6121 ccgttttggt ctggagtttg agaaatcagc ctttggtaag tacaacgttg ataatgctgt
     6181 caacgccagc ttccgttatt cattctgacg gttgttactg aaggggatta tggcctgacg
     6241 gctccctgta aataaagaca ggcacagaag attgtgagtt actcagtgtt ccaccaaaaa
     6301 gtaaggtcgc aactctgtgc ctgcttcgta agtgtgcagt gagggccata tggttataac
     6361 tgaagcatcc gacgacgtag tgtctaccag agtgtcagat atgcaggaaa atatgagttc
     6421 cgacagaaat gcagtaaaaa aaggagtctc gcactcccct ccataaaact ccagggctgt
     6481 aagttgtatt aaaaagctga tttttcttta gcgatacaga cctcaataag tataacaccc
     6541 ttcagttact gtggttatgc acaggtgtta tttttaacag tgtggataaa cagtgtcgat
     6601 tattcagatt ggttatgggg catgtatggg acatttttaa tcggcgaaat acgtcgaagt
     6661 tcgtcgatat ggtaaacgaa tcatctatcc aacccttgaa aaacggcgct cctggacgat
     6721 cttcgtcgat ttttaaaatg caacaagtac gttgttcatg taataggcta ccttgctaca
     6781 gcaagctctt gacataactg tcaatgtcgc tgatgtattt ttctattcct ggaacatcct
     6841 tctcacgaat cagatgttcc agcgtttcac ataactgctt gccgggtacc agatttagca
     6901 tggcaaatac gcctttaaga cgatgagccg tttgggctaa cgcagcaaag tcactggtcg
     6961 ctgcttcagt atacagcctc ttaacatcat ccggtactgt gtctacaaag agcgcataat
     7021 agccgctggc atggagttgc gcattttcat ctccgcccag aggcgattct gtcacctctt
     7081 cctgcgccag ttgcacttca attaattgta agaccgcttc ctgcatagcg ttgctcatat
     7141 tgaagttgac gcacaattga ccaggcccaa tttcccgtac gccagactca tcatcgctta
     7201 aaagcaagcc agaggcagta agattagacg gattatccgt taaaaagata tcataatctt
     7261 gactaattaa tctttcatcg ggtgtgatac aggttgcacc ccaattttct aactggcgag
     7321 tgacaatatt ccgaatttct gccgaagtaa catccaccat tacgcagaca tcatccagta
     7381 aacgctcttc ttcctcttca acttccgggt cagctgcgag cattttgata tgcacagagt
     7441 agcgagtacc aagcccatcc cgcgttttga tgtttaaatg accgcccagt ttacgcgcca
     7501 gttgatcgct cagccagaat gccagcgggt ccgccttgcc atagcgatcg ttttgggtct
     7561 ggttgataaa cgggaagtgc aaattatcca tttcatgaat gcttacgcct tctccagtgt
     7621 ccagaatacg gaacgtcagg cggtcttcgg aggactcatc ctgatcaacc tcaagggtga
     7681 tttttcccaa ttgcgttgag gtcacggcat attgcatcag taacagcaaa atacgtcgta
     7741 aggcatcgcg atcgccgcgg cgcatgtcgt gtgctttcag atgattgtta atcagcagtt
     7801 gcagaccttt acgcttgatg gcaggcaaca ctgaaggcac aacttcatca attaaatcct
     7861 gcacggagaa cagcaccgtc tcacttttcc agctatcgtc cgcaagcatg ttcgctaact
     7921 gtatttcatc gaccagccgc accagcacat ccgcctgatt cgtcagttgt ttgccttccg
     7981 gggcgttgag tttagccgcg ctctccgcca gggactgtgc gggttctttc agcgcatcgc
     8041 caatgttttt cataaaggtc atccgcccct gctggttttt ctcatacaga cgctgcgcct
     8101 gcttgagttt cttgtttacc agcacttcgc gatcctgatc gcgaataatg aaaatttgtg
     8161 tgcgcggcgc aacctggctg cggaacatgc ggatctcata cagctcgtta ttgatcgtcg
     8221 cctgaataat cccctgatgc tgttccgcca tggtggtgat gttttgcaga ttcaaatgcg
     8281 gcagcaaatg atcggcaatt ttgttactta tgacagtgcg gttcgattcc tgatcgtgaa
     8341 ccagcaggcc gagcggcagc agtgagacta tctcttcatt gattgcccgt aaaatgcgca
     8401 attcgttatt gaccgccgtg ttgggtagac tttctgtact gcgactggag aaatggcgaa
     8461 atgtggtata gccaaataac gccagcgcca gcaaaccgat gttcagcagc agtggcagca
     8521 gaatgttttg caacgtatcc agcaataagg tgccataagg aacctgccag accaggcgca
     8581 tatcggtaga gttgagtgcc gaggagattt caatcttcgt actgttaaag tggatactga
     8641 cgctatccgt cccttctttc tcattatcat tgtctcccgt tgccgtcgcg tctggctcaa
     8701 ggcggaaact gtccagcggc atacccggtg ggatcaaatc attaatcggc agatcaaaag
     8761 ccacaaccgt tgccagatgt cccggctggt tgaacgtggt acgcaaggta aagtaatgac
     8821 cgttctgcca ggccaggcgg cgcatgttag aaaagctttc gcgttcatcg agggcgttgg
     8881 cctgttgcaa catctctgca cgacgtgaat caacaatgtc gctgacggtc gattctttaa
     8941 atccggaggt gagatctttg aggggcaggg ttgagatcag caccagactg ttatcctgac
     9001 cattcaggta atacatcgac cacggtacat tttctgcgcc ccacaatgta tccagataag
     9061 tggacatccg ttgagtcatc tcaagcgttg agttgtcgtg agagccaaag attaacgctt
     9121 ccgttttgcg gcgtggtttc tccagatagt agacatcctg tttcaggcgc gtctcttgta
     9181 aaccttcgcc ggaggagggg gtggtcgtcg cggcaatgtt gtcgtagatc tgccaggtca
     9241 cgtaacgcca ggtatcgacg cgcttttgga tagcgtgggt aatgtcgaca atctggtaac
     9301 ttttatcttt cagccaggcg ttaacggcgc tttgtaccat tacacccatc gtcaccagta
     9361 acacaatgat caacagtaaa aagaagcggg taatgctccc cggtaggagt gaaaagcggg
     9421 tcgtggccgt tgtctctttc tgacgcattc gtgtttatga cctgttaaaa cttcgcgaag
     9481 tgatagggca gtataaaggg tacagtgtga aattccagac tcttaccgga ttgatgattc
     9541 tctattctcc cgtgatattt atcatcctgc attgggggaa atagatgtac aagcgccatt
     9601 ttgctgtaca aataacctac aaaaagccta acgaaattaa ttgcaaataa acgaagattg
     9661 ctggaaatta tgctgatgtt aattatttgt gaaatagtta acaagcgtta tagtttttct
     9721 gtggtagcac agaataatga aaagtgtgta aagaagggta aaaaaaaccg gatgcgaggc
     9781 atccggttga aataggggta aacagacatt cagaaatgaa tgacggtaat aaataaagtt
     9841 aatgatgata gcggaagata ttctagttgc gagtgaaggt tttgttttga cattcagtgc
     9901 tgtcaaataa ttaagaa
//
LOCUS       DAZGQL010000149         9878 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.149, whole genome shotgun sequence.
ACCESSION   DAZGQL010000149 DAZGQL010000000
VERSION     DAZGQL010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9878
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.149"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(12..737)
                     /gene="phoU"
                     /locus_tag="ACHKIR_004672"
     CDS             complement(12..737)
                     /gene="phoU"
                     /locus_tag="ACHKIR_004672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFO9992313.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAEKDSDK"
     gene            complement(752..1525)
                     /gene="pstB"
                     /locus_tag="ACHKIR_004673"
     CDS             complement(752..1525)
                     /gene="pstB"
                     /locus_tag="ACHKIR_004673"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFO9992314.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(1616..2506)
                     /gene="pstA"
                     /locus_tag="ACHKIR_004674"
     CDS             complement(1616..2506)
                     /gene="pstA"
                     /locus_tag="ACHKIR_004674"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFO9992315.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMIAAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(2506..3465)
                     /gene="pstC"
                     /locus_tag="ACHKIR_004675"
     CDS             complement(2506..3465)
                     /gene="pstC"
                     /locus_tag="ACHKIR_004675"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HFO9992316.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALTVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(3552..4592)
                     /gene="pstS"
                     /locus_tag="ACHKIR_004676"
     CDS             complement(3552..4592)
                     /gene="pstS"
                     /locus_tag="ACHKIR_004676"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFO9992317.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(4840..5910)
                     /locus_tag="ACHKIR_004677"
     CDS             complement(4840..5910)
                     /locus_tag="ACHKIR_004677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO9992318.1"
                     /translation="MNKYIIPWCFIVFIFSFSGATFAAPKGICTSDNGSFHSTLDFSG
                     YLITADENRVGKTFNKTVTNGDSYPAHCHCDTGNVGEFPYIYYTARINESLGYAGVRS
                     NVNYFNLNPNLDVGISIDLLGVGYINAPFEYHANRPTGGSYKCSRTDPLSISSGAKAI
                     IYFYIKKTFAGKLIIPETLVAKLYGTISRDTPVDYSQPMADVYIRGDITAPQSCEINS
                     LRPINFDFKEIPAADFSSVVGSTVTTHKITKTVTIECENLGILNTDDISTSFYATEPS
                     TDNSMVVTSNPNVGIKIYDKNNKEINVNGGELPTDMGKSTVFGEKAGSVTFSAAPASL
                     TGARPAPGTFTATATITIEIVR"
     gene            complement(5921..8443)
                     /gene="lpfC-O113"
                     /locus_tag="ACHKIR_004678"
     CDS             complement(5921..8443)
                     /gene="lpfC-O113"
                     /locus_tag="ACHKIR_004678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial usher protein LpfC-O113"
                     /protein_id="HFO9992319.1"
                     /translation="MMTTRIVVGLTAGTCLIFSQSLMAEVSVFNPALLEIDHQSGVDI
                     RQFNRANLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFVAIKKTLTTFGVK
                     VDALKSLNDVDETVCIDPGPRIEGSSWQFDSDKLQLNISIPQIYMDAMAYDYISPSRW
                     DEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTTDGS
                     AEYNSISTWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFA
                     PVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDLDVTIKEEDGSEQ
                     RFTQPYASLAILKREGQTDVDVSVGELRDEDGFTPDVLQVQILHGFPYGFTLYGGMQA
                     AEKYGSAALGVGKELGALGAISFDVTHARAKFSHADTETGQSYRFLYSKRFDDTDTSL
                     RLVGYRYSTEGYYTLNEWASRRNNPEDFWETGNRRSRVEGTLTQSLGRDYGNLYLTLS
                     RQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSYSNTARQGTGNNHASDNTSEQIYMLS
                     LSVPLSGWWGNSYATYSVSQNDNSGSSHQLGLSGTALERNNLSWNLMQSYNSHDDEVG
                     GNMSLTYDGTYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVKAP
                     GAAGLEIDNMRGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVVTMVPT
                     RGAVVKAEFVTRVGYRVLFRVAGTKGKPAPFGAIATVQNTSSADSGIVGDQGELYLSG
                     LPEKGQVMLSWGENAATTCTFDYSLSIPESESGLIEQGVTCH"
     gene            complement(8468..9199)
                     /gene="lpfB-O113"
                     /locus_tag="ACHKIR_004679"
     CDS             complement(8468..9199)
                     /gene="lpfB-O113"
                     /locus_tag="ACHKIR_004679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_316045823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial chaperone LpfB-O113"
                     /protein_id="HFO9992320.1"
                     /translation="MSRKSCLIRLFKTTVSCKLFTAIILSVFLVQPALTYAGVVIGGT
                     RVVYLSNNPDKSISVFSKEEKIPYLIQAWVDPFNKEDKSKAPFTVIPPVSRLEPAQEK
                     VLRIIHTKGVSLPDDRESVFWLNIKNIPPSASNKAANSLEIAVKTRIKLFWRPASIRL
                     IPEDAAPKVKWHREGRNLIAENPNPIHISVMDVIVDGHDVPLNMIRPFETLTLPLPAN
                     SAGGQMTWRFINDYGAVSDPIKMTL"
     gene            complement(9247..9819)
                     /gene="lpfA-O113"
                     /locus_tag="ACHKIR_004680"
     CDS             complement(9247..9819)
                     /gene="lpfA-O113"
                     /locus_tag="ACHKIR_004680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA-O113"
                     /protein_id="HFO9992321.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVHFVGEIVDTTCEVTSD
                     TADQTVPLGKVSKNAFSGVGSLASPQQFSIKLEKCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
BASE COUNT         2532 a         2514 c         2315 g         2517 t
ORIGIN      
        1 tacttggtga attatttatc gctatctttt tctgccagca gtttatcgag ctcgtcacca
       61 ccgacgtgac ggaaatcctg ccccttcacg tagtagaaga taaactcaca aatattctgg
      121 cagcggtcgc cgatacgttc gattgaacgc gcacaaaaca gcgcggtaag tacgcttgga
      181 atggtgcgcg aatcttccat catgtaggtc atcagctgac gaacaatacc ttcgtactcc
      241 tgatcgactt ttttatcttc acgataaata cgtaccgctt catcaatgtc catccgcgcg
      301 aacgcgtcca gcacgtcgtg cagcatctgg atggtatgac ggcccagcga ctccagactt
      361 accagcaatg gctgatgctg ctgggagaat ttctccagtg cagtacggca gattttgtcc
      421 gccacgtcgc caatacgctc cagctcggca atggttttac tgatcaccat aaccagacgc
      481 aggtcgctcg ccgtcggctg gcgtttggca ataatgcgca cgcaggcttc atcgatcgcc
      541 acttccatca tgttgacgtt cttgtcgcct tcgatgacgc gcttcgccag atcgctgtcc
      601 tggttgtgca ttgcagtgat cgcatcagaa agctgctgct ccaccatgcc gcccatggtc
      661 atcacctgcg tgcggatgct ttccagttcg gcgttgaact ggccggaaat atgtttatta
      721 agattgagac tgtccataac gcactcctga atcaaccgta acgaccggtg atgtagtctt
      781 ctgtttgttt cttcgctggc ttggtgaaca gatcgtccgt gttgctgaac tcaatcaatt
      841 cgcccaggta cataaacgcc gtgtggtcgg aacaacgcgc agcctgctgc atgttgtggg
      901 tgacgatcac cacggtgtaa tcctgcttca gttcggtaat cagctcttca atacgcccgg
      961 tggagatggg gtcgagcgcc gaacatggtt cgtcgagcag cagcacttcc gggcgaatgg
     1021 cgataccacg cgcaatacac aaacgctgtt gctgaccacc agagagagag taaccgctct
     1081 ggtgcaattt atctttggtt tcgttccaca atgcggcttt ggtcaatgcc cactgcacgc
     1141 gctcgtccat gtcagcacgg gagagcttct caaacagacg cacgccaaaa gcgatgttgt
     1201 cgtagatgga catcgggaac ggcgtcggtt tctggaacac catgcccact ttcgcacgca
     1261 gcagggcgat atcctgagag ttggtcagga tgttgtcgcc atcgagcaga atttcacctt
     1321 ccgcacgctg ttccgggtac agttcaaaca ttttgttgaa ggtacgcagc agcgttgatt
     1381 taccgcagcc ggacggcccg ataaacgccg taacctggtt tttggcgata tccaggttga
     1441 tgtttttcag ggcatggaat ttgccgtagt agaagttcaa attacgaacc tgaattttac
     1501 tcggggcagt ttcaaccata ctcattgcaa tcttttcctc atcgtgcctg atgcacttcg
     1561 cgaatcaggc ttaccgtagg ccggataagg cgtttacgcc gcatccagca aagtgttaac
     1621 cgtgtttatt cttcgcaaaa acaacgcgcg ccagaatgtt cagcagcagt acgcacaggg
     1681 taatgatcaa caccccggcc caggccagtt gctgccattc cgcaaacggg ctcatcgcaa
     1741 acttaaagat cgtcaccggc aggttggcga tcggctgcat catgtccgtg ctccagaact
     1801 ggttggagag cgcagtaaac agcagcggcg cggtttcacc agcaatacgg gcaatcgcca
     1861 gcaggatacc ggtcatgatc ccggacaccg acgctttcag cgtgatcgcg gcaatcatct
     1921 tccacttcgg cgtacccagc gcataagccg cttcacgcag gctgtccggc accagtttca
     1981 gcatgttctc ggtggtgcgg ataacaatcg gcacctgcaa cagcgccagc gcaatcacgc
     2041 ccgcccagcc ggagaagtgc tccatctgcg ccaccacgat ggtgtaaaca aaaagaccaa
     2101 ccacaatcga cggtgcggag agcaaaatgt cgttaatgaa gcgaatcact tctgccagcc
     2161 aggatttacg accatattca gccagataaa tccccgccat aatgcccagc ggcgtaccga
     2221 ataccgtggc ccacaaaatt aacaggccgc tacccgccag ggcgttcgcc agaccaccgc
     2281 cttccgtatt tggcggcggc gtcatttcgg tgaacagcgc cagcgacata ccatcgatac
     2341 cgcgagtgat ggtggacatt aaaatccaga tcagccagaa cagaccgaag gccatcgtcg
     2401 ccatcgagag cgtcagcgca atacggttct taaggcggcg acgcgcctgc attttacggc
     2461 gggattcagc cagcgccgca gtggtttgca tttcaaccat agccattagc gtgccccctc
     2521 attcttagcc aggcgcataa tcataaactt cgatgcggcg aggacgatga aggtaatcac
     2581 aaacaggatc aggcccagtt ccatcagtgc agcaacatgc agacccgact ctgcttccgc
     2641 aaattcgttg gccagcgcag aggtgatact gttgcccggc atatacagcg aggcgctgtc
     2701 gagttggtag gtgttaccga tgataaaggt caccgccatg gtttcaccga gcgcgcgccc
     2761 cagccccaac atgatgccgc cgataacacc atttttggtg aacggaagaa cgatacgcca
     2821 gataacttcc caggtggtgc agccaatacc gtaggccgac tctttcatca tcaccggggt
     2881 ttgttcgaat acatcgcgca ttaccgccgc aatgtacgga ataatcatga tggcgaggat
     2941 cacgcctgcc gcgaggatac cgataccaaa ggccgggccg gagaacagcg cgccgacaat
     3001 cgggatattc gacatgatat tgccgaccgg ctcctggaag taaacggcga acagcggggc
     3061 gaagataaac aagccccaca tgccgtaaac gatacttgga atggcagcca gcagctcaat
     3121 ggcgataccc agcgggcgtt tcagccagcc aggcgcaagc tcggtcagga acagggcgat
     3181 accaaaactc accgggacgg cgatcagcag cgcgataaac gaggtcacca gcgtaccgta
     3241 gatcggcacc agcgccccgt agatatcgtt cggcgcatcc cactctttgg tccacaggaa
     3301 agccagacca aatttctgaa tgctcggcca ggatgagatg atcagagaga caataatgcc
     3361 acccaacatc aatagcacag tcagcgccgc cagttttacc agcacgctga aaattatgtc
     3421 gcccttttta cccggtgggt taaaagcagg cttggttgca gccataagtt actcttcagt
     3481 taaacgcgtt tacaaagtca ttttgtaatg ccggatgcgg cgtaaaacac cgtacccggc
     3541 ctggagtttt attagtacag cggcttgccg ctactgtctt taatattggt cttccacgca
     3601 gcgcgaacct gttcaactac gctatccggc aggctggcat aatccaggtc gttcgcctgt
     3661 ttagccccgg ttttgtatgc ccagtcgaag aacttcagca cttcggtgcc ttgttctggt
     3721 ttcttctgat ctttatggat cagaatgaac gtggtagagg taataggcca tgcatcttcg
     3781 cctttctggt tggtcagatc ctgagcgaat gttttgctcc agtctgcgcc ttttgctgca
     3841 ttagcgaagt tttcttcggt cggactcacc ggtttgccat cagcggagat cagtttggtg
     3901 tacgccaggt tgttctgctt cgcgtaagca tattcaacgt aaccaattgc acccggcaga
     3961 cgctgaacga acgcggcgat accgtcgtta cctttaccgc ccagaccgat cggccatttt
     4021 acggtagagc cagtaccaac gttgtttttc cactcttcgt tcactttcgc caggtagctg
     4081 gtgaagacga aggaagtccc ggagccatct gcgcggcgta ctacagcaat gttctgtgaa
     4141 ggcagtttca gacccggatt cagtttggcg atggcttcat catcccactt cttgattttg
     4201 cccaggtaga tgtcgccgag ggttttacca tccagcacca gttcgccaga cttcagccct
     4261 ggaatgttaa ccgccagcac cacgccgcca atcacggtcg ggaactggaa caggccttcc
     4321 tgagccagtt tttcgtcaga cagcggcgcg tcagaggcac cgaaatcaac ggtattagcg
     4381 ataatctgtt ttacgccacc ggaagaaccg ataccctggt agttaacttt attaccggtt
     4441 tctttctggt aagtgtcagc ccatttggca tacaccggcg cagggaaggt tgcacctgca
     4501 cctgtcaggc ttgcttctgc aaacacagag aaagcactca tcgataaggt cgcggcgaca
     4561 acagttgcga cggtggtacg cataactttc ataatgtctc ctgggaggat tcataaagca
     4621 ttgtttgttg gctacgagaa gcaaaatagg acaaacaggt gacagttata tgtaaggttt
     4681 atgacagttt tatgacaaat cagaattatt aattaacttt ttgattttat tgactaagaa
     4741 aaagaaaagt aatgtcatct ttttgtcatg cagagtgaaa actcgcggac aaaaatgcgt
     4801 aagatgacaa cggtgtgagg aacaccgctg tcagttcgtt tagcgtacaa tctcaattgt
     4861 tatcgttgca gtcgcggtaa atgttcctgg cgcggggcga gcccccgtaa ggcttgcagg
     4921 agcagcagaa aaagtgacgc taccggcttt ttcaccaaag acggttgatt tacccatatc
     4981 tgtcggcagt tcaccaccgt taacattgat ttccttatta tttttatcat aaattttaat
     5041 cccaacgttc gggtttgatg tcacgaccat tgagttgtca gtactgggtt cggtagcata
     5101 aaaagaggta ctgatatcat cagtatttag tattcctaaa ttttcacact caatggtgac
     5161 ggttttggta attttatgcg ttgtcacggt acttccaact accgaagaaa aatctgccgc
     5221 aggaatttct ttaaaatcaa aattaatggg tcttaaactg ttaatttcac agctttgcgg
     5281 tgcagtgata tcgccacgaa tataaacatc cgccataggt tgtgagtaat caaccggagt
     5341 gtcacggctt atggttccgt acaatttcgc cacgagagtt tcaggaataa ttaactttcc
     5401 agcaaaagtc ttcttaatat aaaaataaat tattgctttt gcaccactgg aaatacttaa
     5461 tgggtccgtg cgactacatt tatatgaacc tccagtaggc ctgttagcat ggtattcaaa
     5521 aggtgcatta atgtatccta caccgagaag gtctatcgat attccaacat ccagattagg
     5581 attcaaatta aagtagttta cattagaacg aacacctgca taacctaagg actcgtttat
     5641 tctcgctgta taataaatat aaggaaactc tcccacgttc cctgtatcac aatggcagtg
     5701 agcaggataa gagtccccat ttgtcacggt tttattaaag gtctttccca ctctgttctc
     5761 gtctgccgta atcagatagc cggaaaaatc aagtgtgcta tgaaaggacc cattatctga
     5821 agtgcagata ccttttgggg ccgcaaatgt tgcgccacta aaggagaaga taaaaacaat
     5881 aaagcaccat ggtataatat acttgttcat aaaaatcctt ttaatgacat gtcacacctt
     5941 gttcaattaa gccgctttca ctttctggta ttgaaagtga ataatcgaag gtgcatgtag
     6001 tggcggcatt ttctccccag gagagcatca cctgcccttt ttcaggaagg cccgagagat
     6061 aaagctcccc ctggtcaccg acaattcctg aatcagcgga gcttgtattt tgtactgtag
     6121 caatagcgcc aaaaggtgct ggtttacctt ttgtacccgc aaccctgaaa agcacacgat
     6181 aaccaacacg agtaacaaat tcggccttaa ccactgcacc acgggtggga accatcgtaa
     6241 cgacggtatt atcaagttct atattcgact tcgagaaata gttatcgctg attgctaccc
     6301 ggttttcatc ataagggttt agctgtgtct tgaccgtata gccgcgccag tccgtcgcag
     6361 caccgcgcat attgtctatt tctaacccgg cagcccctgg tgctttaaca agagctatgg
     6421 tttcacccaa ctcctggctt aacgttacgc cttcgctgtg cgccagaatt ccccctctga
     6481 taccataatt cagcctctga gaattttggc tgtagttata gctgccgttc accgtgccat
     6541 aagtgccatc ataggtcagg gacatattac cgccaacctc atcatcatgg ctgttatagg
     6601 actgcattaa attccaggaa aggttatttc tttccagcgc cgtaccgctg agtccaagtt
     6661 gatgtgagct accggaatta tcgttttgtg aaacagaata ggtggcgtaa ctatttcccc
     6721 accagcccga taaaggaaca gataaagaga gcatgtagat ctgctcactg gtgttatcac
     6781 tggcatggtt gttccccgtc ccctgccggg cagtattgga ataactccag gagacgttcc
     6841 acgagagacg cttccagcta ctgctgtagc caaattgcat taatcgttca acatcatcgg
     6901 tatgccagta ctgctggcga cttaatgtca gatataagtt gccatagtct ctgcccagtg
     6961 attgcgttaa cgttccttcc acacggctgc ggcggttacc ggtttcccag aaatcttcag
     7021 gattattacg ccgcgacgcc cattcattga gagtatagta accttcggtg gagtaacgat
     7081 agccaaccag gcgcaagctg gtatccgtat cgtcaaagcg tttcgaatac agaaagcgat
     7141 aagactgacc ggtttccgta tcagcatggc taaatttcgc acgggcatgt gtcacatcga
     7201 aagaaattgc acccagtgcg ccaagctctt taccgacacc caaagcggca gaaccatact
     7261 tttcagcagc ctgcatacct ccatacaagg tgaaaccgta gggaaaacca tgaaggatct
     7321 gcacctgaag aacgtcaggg gtaaagccat cttcatcgcg taattctccc acactgacat
     7381 caacatcagt ttgaccttca cgtttgagga tggctaacga cgcataaggt tgagtaaatc
     7441 gttgttcgct accgtcttct tctttgatgg ttacgtccag atcaccacct gtactggcgg
     7501 tgttgaggtc ggtaatttca aaagcacctt gtggaacggc tgactgataa atcacgtagc
     7561 cattctggcg aataatgaca gtggcgttag actttgcaat cccacgcact acgggggcaa
     7621 atccgttttg gctggcaggc aacatatcgt tatcggtata caagcgtgcg ccacgaattt
     7681 gcgtactgtc gaagatatcg ctcgccgtcc acgtatcacc tatcataatc tggcttctta
     7741 acgcagctat atcacgttgt atccaggtgc ttatagagtt gtattctgca ctgccatcgg
     7801 tcgtatttaa ggtgctgtaa ttgcgtaatc gccacggccc aatattcagt ccattgcgca
     7861 gattgagata actggtatct gtctcttgtg aaccataatc cgaacgtaga gtatgtgaac
     7921 cagaaaaatc gtagttgatg gtgagcgcgt taatcccctc atcccaacgc gaggggctga
     7981 tgtaatcata agccatcgca tccatataga tttggggaat ggagatattc agttgcagtt
     8041 tatcgctgtc gaactgccat gatgagcctt caatgcgtgg cccaggatca atacagactg
     8101 tttcatctac atcattgagc gatttcagcg catcaacttt tacgccaaag gtggttagcg
     8161 tttttttaat agcgacaaag caggcgtgta aatcagcatc gggattatcc tgaacaaagg
     8221 tcacatcctg acgttcaaac attttaccgt tgataaaaat atcaacgcta taaacaccag
     8281 ggggcatcag gtttgcccga ttaaactggc gaatatcgac tccggattga tggtcgattt
     8341 ccagaagcgc cggattgaat acactgacct cggccatcag gctttgcgag aaaatcagac
     8401 acgtccctgc cgtgaggcca accactattc tggtcgtcat cataatacgc ctatccttat
     8461 cccttgctta tagcgtcatt tttatcgggt cactgactgc gccgtaatca ttaataaatc
     8521 gccaggtcat ttgacctccc gcagaattag caggtaaagg taacgtgagc gtttcaaaag
     8581 gacggatcat gttgaggggc acatcatgac catcaacgat gacatccatc acactgatat
     8641 gaatcgggtt gggattctct gcaatcaggt ttcgcccttc ccggtgccac ttcaccttcg
     8701 gtgcagcatc ttcgggaatc aggcgaatgc ttgccggtcg ccagaacagc ttgatacgcg
     8761 ttttaactgc gatttccagt gagttcgccg ctttgttgct cgcagagggc ggaatatttt
     8821 taatattcag ccagaagact gactcgcgat catcaggcaa tgacacacct ttggtatgaa
     8881 taatgcgtaa tactttttct tgcgcgggtt ctaatcgtga taccggagga ataacggtaa
     8941 acggtgcttt gcttttatcc tctttattaa acggatctac ccatgcctgg atgagataag
     9001 gaattttttc ttccttgcta aatacagaaa tagatttatc tgggttatta cttaagtaaa
     9061 ctacccgtgt accaccaatg acaacgcctg cataggttaa tgctggttgg actaaaaaaa
     9121 cactgagtat aatcgcggta aataacttac atgaaacggt tgttttaaag agcctgatta
     9181 aacaactttt tctggacata acgactccct ggccaaagcc aactgaataa aaaggccctt
     9241 gataaattat ttcttatatt cgacagtaaa ttgtgaatcg gcgttcgccg tacccgccgt
     9301 tacggttgca ttggttgcca catagcgtgc aataaacttc atttcagcct tgccttcagc
     9361 gtcgatagcg gtataggcag aatcgttata aagtttaacc tgcgagttat ctgactggtt
     9421 aaaaatacga ataccgacac ccgttgccgc aatcgcttgt cctgtaccgg taaaatcgcc
     9481 agggttgccc gctgtaagcg gcgtacccac tttcaggtcg ccgtcgccgc caggcgcttc
     9541 tgtaccatca aaacgaacgg ctgcctgagt gtaggttgcc gggcactttt caagtttaat
     9601 actgaactgc tgtggtgacg ccagactacc tacacctgaa aatgcatttt tactgacttt
     9661 acccagtggg acggtttgat cggctgtatc ggaggtaact tcacaagtcg tgtcgacaat
     9721 ttcaccgacg aagtgaacaa caccatcttc tgccagtgca tgtccggcca gcattaaaga
     9781 tgccagagtg aatgcaccgc ctataatatt acgcttcata aaaactccat tgatataata
     9841 tatcctttac catgaatcat cacttatatt aataagtg
//
LOCUS       DAZGQL010000150         9814 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.150, whole genome shotgun sequence.
ACCESSION   DAZGQL010000150 DAZGQL010000000
VERSION     DAZGQL010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9814
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.150"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            108..158
                     /gene="hisL"
                     /locus_tag="ACHKIR_004681"
     CDS             108..158
                     /gene="hisL"
                     /locus_tag="ACHKIR_004681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HFO9992322.1"
                     /translation="MTRVQFKHHHHHHHPD"
     misc_feature    134..257
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            304..1203
                     /gene="hisG"
                     /locus_tag="ACHKIR_004682"
     CDS             304..1203
                     /gene="hisG"
                     /locus_tag="ACHKIR_004682"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HFO9992323.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            1209..2513
                     /gene="hisD"
                     /locus_tag="ACHKIR_004683"
     CDS             1209..2513
                     /gene="hisD"
                     /locus_tag="ACHKIR_004683"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HFO9992324.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     ARGDEALREYSAKFDKTTVTALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETARQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARDLVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            2510..3580
                     /gene="hisC"
                     /locus_tag="ACHKIR_004684"
     CDS             2510..3580
                     /gene="hisC"
                     /locus_tag="ACHKIR_004684"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFO9992325.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLDNWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVAQIIAER
                     EYLIAALKEISCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            3580..4647
                     /gene="hisB"
                     /locus_tag="ACHKIR_004685"
     CDS             3580..4647
                     /gene="hisB"
                     /locus_tag="ACHKIR_004685"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HFO9992326.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDLANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQITTHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            4647..5237
                     /gene="hisH"
                     /locus_tag="ACHKIR_004686"
     CDS             4647..5237
                     /gene="hisH"
                     /locus_tag="ACHKIR_004686"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HFO9992327.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLARRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            5237..5974
                     /gene="hisA"
                     /locus_tag="ACHKIR_004687"
     CDS             5237..5974
                     /gene="hisA"
                     /locus_tag="ACHKIR_004687"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HFO9992328.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPARRQIPLIKTLVADVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPEMVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            5956..6732
                     /gene="hisF"
                     /locus_tag="ACHKIR_004688"
     CDS             5956..6732
                     /gene="hisF"
                     /locus_tag="ACHKIR_004688"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HFO9992329.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            6726..7337
                     /gene="hisIE"
                     /locus_tag="ACHKIR_004689"
     CDS             6726..7337
                     /gene="hisIE"
                     /locus_tag="ACHKIR_004689"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HFO9992330.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TLESGKVTFFSRTKQRLWTKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(7432..8409)
                     /gene="wzzB"
                     /locus_tag="ACHKIR_004690"
     CDS             complement(7432..8409)
                     /gene="wzzB"
                     /locus_tag="ACHKIR_004690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HFO9992331.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIVTQPDVGQIAGYTNAMNVIYGSAVPKVSDIQASLIGRYSTA
                     FSALAETLDNQEEAEKLTIEPTVKNQSLPLAVSYVGETPEGAQKQLAKYIQQVDDQVN
                     EELEKDLKDNIALRMKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIENLKVDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(8556..9722)
                     /gene="ugd"
                     /locus_tag="ACHKIR_004691"
     CDS             complement(8556..9722)
                     /gene="ugd"
                     /locus_tag="ACHKIR_004691"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HFO9992332.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEAYRDADYVIIATPTDYDPKTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVPVGFTEAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
BASE COUNT         2434 a         2522 c         2574 g         2284 t
ORIGIN      
        1 ttaaacaaat ccattgccat aaaatatata aaaaagccct tgctttctaa cgtgaaagtg
       61 gtttaggtta aaagacatca gttgaataaa cattcacaga gacttttatg acacgcgttc
      121 aatttaaaca ccaccatcat caccatcatc ctgactagtc tttcaggcga tgtgtgctgg
      181 aagacattca gatcttccag tggtgcatga acgcatgaga aagcccccgg aagatcacct
      241 tccgggggct tttttattgc gcggttgata acggttcaga caggtttaaa gaggaataag
      301 aaaatgacag acaacactcg tttacgcata gctatgcaga aatccggccg tttaagtgat
      361 gactcacgcg aattgctggc gcgctgtggc attaaaatca atcttcacac ccagcgcctg
      421 atcgcgatgg cagaaaacat gccgattgat attctgcgcg tgcgtgacga cgacattccc
      481 ggtctggtaa tggacggcgt ggtagacctt gggattatcg gcgaaaacgt gctggaagaa
      541 gagctgctta accgccgagc ccagggtgaa gatccgcgct actttaccct gcgtcgtctg
      601 gatttcggcg gctgccgcct ttcgctggca acgccggttg atgaagcctg ggatggcccg
      661 ctctccttaa acggtaaacg tatcgccacc tcttatcctc acctgctcaa gcgttatctc
      721 gaccagaaag gcatctcttt taaatcctgc ttactgaacg gttctgttga agtcgccccg
      781 cgtgccggac tggcggatgc gatttgcgat ctggtttcca ccggtgccac gctggaagca
      841 aacggcctgc gcgaagtcga agttatctat cgctcgaaag cttgcctgat acaacgcgat
      901 ggcgaaatgg aagaatccaa acagcagctg atcgacaaac tgctgacccg tattcagggc
      961 gtgatccagg cgcgcgaatc aaaatacatc atgatgcacg caccgaccga acgcctggat
     1021 gaagtcatcg ccctgctgcc aggtgccgaa cgcccaacta ttctgccgct ggcaggtgat
     1081 caacagcgcg tagcgatgca catggtgagt agcgaaaccc tgttctggga aacgatggaa
     1141 aaactgaaag cgctgggtgc cagttcaatc ctggtcctgc cgattgaaaa aatgatggag
     1201 tgatcgccat gagctttaac acaatcattg actggaatag ctgtactgcg gagcaacaac
     1261 gccagctgtt aatgcgcccg gcgatctccg cttctgaaag cattacccgc accgttaacg
     1321 atatcctcga taacgtgaaa gcacgcggcg atgaggccct gcgtgaatac agcgcgaaat
     1381 ttgataaaac cacggttacc gcgctgaagg tgtctgctga ggagatcgcc gccgccagcg
     1441 aacgcctgag cgacgagcta aaacaggcga tggcggtggc agtaaagaat attgaaacct
     1501 tccacactgc gcaaaaactg ccgccggtag atgtcgaaac gcagccaggc gtgcgttgcc
     1561 agcaagtcac gcgcccggtc gcttcagttg ggttgtatat tcctggcggc tccgccccgc
     1621 tcttctcaac ggtattaatg ctggcaactc cggcgcgtat tgcgggctgt aaaaaagtgg
     1681 tgctgtgctc accgccgccg attgccgacg aaattcttta cgcggcgcag ctgtgcggtg
     1741 tgcaggatgt gtttaacgtc ggcggcgcac aggccattgc cgcactggcg tttggtacgg
     1801 aatctgtgcc gaaagttgac aaaatcttcg ggccaggtaa cgcctttgtc accgaggcaa
     1861 aacgtcaggt gagccagcgt ctggacggtg cggcgatcga tatgcccgca ggcccgtcgg
     1921 aagtgctggt gattgctgac agcggcgcta cgccggattt cgtggcttct gatttgcttt
     1981 ctcaggctga acacggcccg gactcgcagg tgattttact gacgcccgat gctgatatgg
     2041 cgcgtcgcgt tgccgaggct gtcgaacgcc aactggcaga actgccgcgt gccgaaaccg
     2101 cccgccaggc actgaacgcc agccgcctga tcgtgactaa agatttagcg cagtgcgtag
     2161 agatctccaa ccagtacggc ccggagcacc tgatcatcca gacccgcaac gcccgcgatc
     2221 tggtcgatgg catcaccagc gccggttcgg tatttcttgg tgactggtca cccgaatcgg
     2281 cgggtgatta cgcctccggc accaaccacg tactgccgac ttacggttac accgccacct
     2341 gttccagcct cgggctggca gatttccaga agcgtatgac cgtgcaggaa ctgtcgaaag
     2401 aaggtttctc cgcgctggct tcgaccatag aaacactggc cgccgccgag cgcctgaccg
     2461 cccataaaaa tgccgttact ttgcgtgtta acgcccttaa ggagcaagca tgagcaccgt
     2521 gactattacc gatttagcgc gtgaaaacgt ccgcaacctg acgccgtatc agtcggcgcg
     2581 tcgtctgggc ggtaacggcg acgtctggct gaacgccaac gaatacccca cagccgtgga
     2641 gtttcagctc actcaacaaa cgctcaaccg ctatccggaa tgtcagccaa aagcggtgat
     2701 tgaaaattac gcgcaatatg caggcgtaaa accggaacag gtgctggtca gccgtggcgc
     2761 ggacgaaggt attgaactgc tgattcgcgc tttttgcgaa ccgggtaaag acgccatcct
     2821 ctactgcccg ccaacgtacg gcatgtacag cgtcagcgct gaaaccattg gcgtcgagtg
     2881 ccgcacagtg ccgacgctgg acaactggca actggactta cagggcattt ccgacaagct
     2941 ggacggcgta aaagtggtct atgtttgcag ccccaacaac ccgaccgggc aactgatcaa
     3001 cccgcaggat tttcgcactc tgctggagtt aacccgcggt aaagcgattg tggttgccga
     3061 tgaagcctat atcgagtttt gcccgcaggc atcgctggct ggctggctgg cggaatatcc
     3121 gcacctggct attttgcgca cactgtcgaa agcttttgct ctggcgggcc ttcgttgcgg
     3181 atttacgctg gcaaacgaag aagtcatcaa ccttctgatg aaagtgatcg ccccctaccc
     3241 gctctcgacg ccggtcgccg acattgcggc ccaggcgtta agcccgcagg ggatcgtcgc
     3301 catgcgcgaa cgggtggcgc aaattattgc agaacgcgaa tatctgattg ccgcattgaa
     3361 agaaatctcc tgcgtggagc aggtttttga ctctgaaacc aactacattc tggcgcgctt
     3421 taaagcctcc agcgcagtgt ttaaatcttt gtgggatcag ggcattatct tacgtgatca
     3481 gaataaacaa ccctctttaa gcggctgcct gcgaattacc gtcggaaccc gtgaagaaag
     3541 ccagcgcgtc attgacgcct tacgtgcgga gcaagtttga tgagtcagaa gtatcttttt
     3601 atcgatcgcg atggaaccct gattagcgaa ccgccgagtg attttcaggt ggaccgtttt
     3661 gacaaactcg cctttgaacc gggcgtgatc ccggagctgc tgaagctgca aaaagcggga
     3721 tataagctgg tgatgatcac caatcaggat ggtctgggaa cacaaagttt cccgcaggcg
     3781 gattttgatg gcccgcacaa cctgatgatg cagatcttca cctcgcaagg cgtgcagttt
     3841 gatgaagtgc tgatttgccc gcacctgccc gccgatgagt gcgactgccg taaaccgaaa
     3901 gtaaaactgg tggagcgtta tctcgctgag caagcgatgg atctcgccaa cagttatgtg
     3961 attggcgatc gcgcgaccga tattcaactg gcggaaaaca tgggtattaa tggtttacgc
     4021 tacgaccgcg aaaccctgaa ctggccgatg attggcgagc aactcactaa acgagaccgt
     4081 tacgcccatg tagtgcgcaa caccaaagag acgcagattg acgttcaggt atggctggat
     4141 cgcgaaggtg gcagcaagat taacaccggc gttggcttct ttgatcatat gctggatcag
     4201 atcaccactc acggcggttt ccgcatggaa atcaacgtca aaggcgacct ctatatcgac
     4261 gatcaccaca ccgtcgaaga taccggcctg gcgctgggtg aagcgttaaa aattgccctc
     4321 ggcgataaac gcggtatttg ccgctttggt tttgtgctgc cgatggacga atgccttgcc
     4381 cgctgtgcac tggatatctc tggtcgccca cacctggaat ataaagctga atttacctac
     4441 cagcgcgtcg gcgatctcag caccgagatg atcgagcact tcttccgttc gctctcttac
     4501 accatgggcg tgacgttgca cctgaaaacc aaaggtaaaa acgatcatca ccgtgtagag
     4561 agtctcttta aagcctttgg tcgcaccctg cgccaggcca tccgcgtgga aggcgacacc
     4621 ctgccctcgt cgaaaggagt gctgtaatga acgtggtgat ccttgatacc ggctgcgcca
     4681 atttgaactc ggtgaagtca gccattgcgc gtcacggtta tgaacccaaa gtcagccgtg
     4741 acccggacgt cgtgttgctg gccgataaac tatttttacc cggcgtaggc accgcacaag
     4801 cagcgatgga tcaggtgcgt gagcgcgagc tgtttgatct catcaaagcc tgtacccaac
     4861 cggtgctggg catctgctta gggatgcaac tgctggcgcg gcgcagcgaa gagagcaacg
     4921 gcgtcgattt gctgggcatt atcgacgaag acgtgccgaa aatgaccgac tttggtctgc
     4981 ccctgccgca tatgggctgg aaccgcgttt acccgcaggc aggcaaccgc ctgtttcagg
     5041 ggattgaaga cggcgcgtac ttttactttg ttcacagcta cgcaatgcca gtcaatccgt
     5101 ggaccatcgc ccagtgtaat tacggcgaac cgttcaccgc ggcggtacaa aaagataact
     5161 tctacggcgt gcagttccac ccggagcgtt ctggtgccgc tggcgctaag ctgctgaaaa
     5221 acttcctgga gatgtgatga ttattccggc attagattta atcgacggca ccgtggtgcg
     5281 tctccatcag ggagattatg gcaaacaacg cgattacggt aacgacccgc tgccacgctt
     5341 acaggattac gccgcgcagg gtgccgaagt gttgcatctg gtggatctga ccggggcaaa
     5401 agatccggct agacgtcaaa tcccgctgat taaaaccctg gtggcggacg ttaacgttcc
     5461 ggtgcaggtt ggcggcggcg tgcgtagcga agaagacgtg gcggcgttac tggaagcggg
     5521 cgttgcgcgc gtggtggttg gctccaccgc ggtgaaatca ccagaaatgg tgaaaggctg
     5581 gtttgaacgt ttcggtgccg atgccttagt gctggcgctg gatgtccgta ttgacgagca
     5641 aggcaacaag caggtggcgg tgagcggctg gcaagagaac tcgggcgttt cactggaaca
     5701 actggtggaa acctatcttc ccgtcggcct gaaacatgtg ctatgtaccg atatctcgcg
     5761 cgacggcacg ctggcaggtt ctaacgtctc tttatatgaa gaagtgtgcg ccagataccc
     5821 gcaggtggca tttcagtcct ccggcggcat tggcgacatt aacgatgttg ctgccctgcg
     5881 aggtactggc gtgcgcggcg taatagttgg tcgggcatta ctggaaggta aattcaccgt
     5941 gaaggaggct atcgcatgct ggcaaaacgc ataattccat gtctcgacgt tcgtgatggt
     6001 caggtggtga aaggcgtaca gtttcgcaac catgaaatca ttggcgatat cgtgccgctg
     6061 gcaaaacgtt acgccgaaga aggtgcagac gaactggtgt tctacgatat caccgcttcc
     6121 agcgatggcc gtgtggtaga taaaagctgg gtatctcgcg tggcggaagt aatcgacatt
     6181 ccgttttgtg tggcgggtgg gattaagtct ctggaagatg ccgcgaaaat tctttccttt
     6241 ggcgcggata aaatctccat taactcccct gcactggcag acccgacgtt aattactcgc
     6301 ctggcggatc gctttggcgt gcagtgtatt gtggtcggca ttgatacctg gtacgacgcc
     6361 gaaaccggta aatatcatgt gaatcaatat accggcgatg aaagccgcac ccgcgtcact
     6421 cagtgggaaa cactcgactg ggtgcaggaa gtgcaaaaac gcggtgccgg agaaatcgtc
     6481 ctcaacatga tgaatcagga cggcgtgcgt aacggttacg acctcgaaca gctgaaaaaa
     6541 gtgcgtgaag tttgccacgt cccgctgatt gcctccggtg gcgcgggcac catggaacac
     6601 ttcctggaag ccttccgcga tgccgacgtt gacggtgcgc tggctgcttc cgtattccac
     6661 aaacaaataa tcaatattgg tgaattaaaa gcgtacctgg caacacaggg cgtggagatc
     6721 aggatatgtt aacagaacaa caacgtcgcg aactggactg ggaaaaaacc gacggactga
     6781 tgccggtgat tgtgcaacat gcggtatccg gcgaagtgct aatgctgggc tatatgaacc
     6841 cggaagctct ggacaaaacc ctcgaaagcg gcaaagtcac cttcttctcg cgcactaaac
     6901 agcgactgtg gaccaaaggc gaaacgtcgg gcaattttct caatgtggtg agtattgccc
     6961 cggactgcga caacgacacg ttactggtgc tggcgaatcc catcgggcca acctgccaca
     7021 aaggcaccag cagctgcttc ggcgacaccg ctcaccagtg gctgttcctg tatcaactgg
     7081 aacaactgct cgccgagcgc aaatctgccg atccggaaac ctcctacacc gccaaactgt
     7141 atgccagcgg caccaaacgc attgcgcaga aagtgggcga agaaggcgtg gaaaccgcac
     7201 tggcagcaac ggtacatgac cgctttgaac tgaccaacga ggcatctgat ttgatgtatc
     7261 acctgctggt gctgttgcag gatcaggatc tggatttaac gacggtaatt gagaacctgc
     7321 gtaaacggca tcagtgagtt gcgggtgatc agatgcgatg ttgttgtcgc atccggcaaa
     7381 aaaaacgggc aaggtgtcac caccctgccc gttttcttta aaaccaaaag attacttcgc
     7441 gttgtaatta cgcagagcgt tacgccccag cacaatcccc gcgccaacca tgccgcccag
     7501 cagcactgcc agaatcaagg taattgcctt tttcgggcta tcgcgacgaa taggtaacgt
     7561 cggtttcatc acatagcggt aagcatgaat atcaagatca tcaaccttta agttttcaat
     7621 atccaacagg ttttgacgag tctgatagta gtttggcgag aacaccaacg gacgagtcgc
     7681 ctcatgctta atcatcgact ccagcgcttc gctccccaaa aggaacaacg tatcttgtgt
     7741 cacatcttca gtctgctgaa cctgtggctt tgtcacctgc gcctgattcg catactgcaa
     7801 cgcttcctga atctgacgga tacgcagatc tttctgctct tgcgcgacta cttcctgagt
     7861 tcttaaagag tcctgcaagt ttttcatacg cagcgcgatg ttgtctttca ggtctttttc
     7921 cagttcctca ttaacctggt cgtcaacttg ctggatatac ttcgccagct gcttctgagc
     7981 accttcaggt gtttcaccaa cataagacac cgctaaaggt aatgactgat ttttaacggt
     8041 aggctcaatg gtcagctttt ctgcttcttc ctgattatcc agcgtttccg ctaatgctga
     8101 aaatgcagtg ctgtaacgtc cgataagtga agcctgaatg tcggagactt tcggtacagc
     8161 cgatccgtag atcacattca tcgcgttggt ataaccagcg atttgcccca catcaggttg
     8221 agtaacgata gctgtggaag tccacttttc tttggcaacg gccagatacc ctatagccag
     8281 ggcaatagca acaatgacag aaatgatgat agtcatcttg ccgcgccaca actgcaccag
     8341 taaatcaatc aaatcaatct gttccgggtc atggttttgc ccagaaacat tattattttc
     8401 tactctcatc attaccctaa cggacgaaat aattttaagt cgccatagtt tagcgataat
     8461 cgccatgaat gctataggat aaatgataaa aagaatgaat tattacaaag ggaacatgag
     8521 agaatcaggg cgaaaatcgc cctgataaca ggatgttagt cactgccaaa gagatcgcgg
     8581 gtgtagactt tgtctgccac atccttaagc tcttctgcca tacggttgga aataataacg
     8641 tcggcttgtt gtttgaaggt ggcgagatca cgttccagtc gagagttgaa gaatgaatct
     8701 tctttcatca ccggctcgta gatgatcact tcaacacctt ttgccttgat acgtttcata
     8761 atcccctgaa tggaagacgc gcggaagtta tctgacccgc tcttcataat cagacgataa
     8821 atacccacca cttgcggttt gcgtgacaaa atggcatcgg caataaaatc tttacgcgtg
     8881 cggtttgcat cgacaattgc cgagatcagg ttattcggca cagactggta gttcgccagt
     8941 aactgcttgg tatctttcgg tagacaataa ccaccatagc caaacgacgg attgttgtaa
     9001 tggttgccaa tacgcggatc gagacaaaca ccttcgataa tctgacgagt attcagacct
     9061 aaactttctg catagctatc cagctcgtta aagtatgcta cgcgcatcgc cagataggta
     9121 ttagcaaaca gtttaatcgc ttctgcttca gtggagtcgg taaacagggt cgggatattt
     9181 tgcttaatcg cgccttcctg taataacgcc gcaaaacgtt ctgcgcgttc tgaacgctca
     9241 ccgatgacaa tacgtgacgg atgaaggtta tcgtaaagag ctttaccttc acggagaaat
     9301 tccggagaga aaataatatt ttcagtacga tatttcttat gcatcgcttc ggtaaaacca
     9361 acgggaaccg ttgatttgat gaccataacc gcataaggat ttatctcaac tacgtcttta
     9421 attactgatt ctacactcga tgtattgaaa taattagttt taggatcata gtcggttgga
     9481 gtggcgatga tgacgtaatc agcatcccga taggcttcat ttttatctaa tgtggcatta
     9541 aagtgtattt tatctgattg caaaaactgc tgaatttcct tatcaacaat aggagatatc
     9601 cgatcattca gcatagcaac gcgtgacggt aaaatatcta atgccacaac ctcatgattt
     9661 tgtgcgatta gaagcccgtt tgacaagccg acatagccag taccggaaat ggtgattttc
     9721 atttcatgct ctcagaatta acttaactgt gaatcatgat gtttttagca tcctgataag
     9781 agttaaaagt tttaacgcta cgttgtaaga tggt
//
LOCUS       DAZGQL010000151         9762 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.151, whole genome shotgun sequence.
ACCESSION   DAZGQL010000151 DAZGQL010000000
VERSION     DAZGQL010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9762
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.151"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..475
                     /locus_tag="ACHKIR_004692"
     CDS             <1..475
                     /locus_tag="ACHKIR_004692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFO9992333.1"
                     /translation="CLLNVFVSTVKQLKNSIIAISAIVAMATVMDVCGLIGTLAQSLV
                     DLTGGAYLFIAPVIGALGTFVTGSDTNSNILFGKLQTTAAEKLHVDPLWLAAANTSGA
                     TGGKMISPQSIAIAVSATRMDGQGSVIMSGTLKYCFAYIVILGLKVGLVYHLFMA"
     gene            525..1244
                     /locus_tag="ACHKIR_004693"
     CDS             525..1244
                     /locus_tag="ACHKIR_004693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFO9992334.1"
                     /translation="MNVNFFVTCIGDAFKSRMAQNSVLLLEQLGCNVHFPEKQGCCGQ
                     PAINGGYVRDAIPGMKNLISSLEENDWPVVSPAGSCTYTIKNYARYLSEEPEWAARAE
                     KVAERLHDLTSFIVNHLGITDVGARLNGRAVYHPSCSLSRKLGVTREPLLLLENVRGL
                     ELLPFHAQETCCGFGGTFSVKMAEISGEMVKEKVGHIMDVKPDFLIGADASCLLNIGG
                     RLSREGHPVKVMHIAEVLMHR"
     gene            1255..2670
                     /locus_tag="ACHKIR_004694"
     CDS             1255..2670
                     /locus_tag="ACHKIR_004694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFO9992335.1"
                     /translation="MSLKTSDIRFKERIRIQIQDPIMRKAVANAQERIGTNRQKMVDE
                     LGHWEEWRSLAEQIRRHVLENLDAYLYQLSEKVTANGGHVYFARTREEATSYILKVAK
                     EKNARKVVKSKSMVTEEIGMNGVLQKAGIPVIETDLGEYILQLAHEPPSHVVVPAIHK
                     DRHQIRRVLHEHLGYDGPETPEAMTLFIREKIRQDFLSAEVGVTGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFREVDVLITMLARSAVGARLTGYNTWLTG
                     PREEGHVDGPEEFHLVIVDNGRSEVLGSEFSDVLRCIRCGACINTCPAYRQIGGHGYG
                     SIYPGPIGAVISPLLGGYEDFKELPYACSLCTACNGVCPVKIPLAQLIQKHRQVMAES
                     EITPSTERRITRIFNYVNSHPALWKVGMIAGAKTASLCIRNGKTPISPGAIKEWSVAR
                     DLPSPDGESFRSWFKRHKKGG"
     gene            2675..3370
                     /locus_tag="ACHKIR_004695"
     CDS             2675..3370
                     /locus_tag="ACHKIR_004695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFO9992336.1"
                     /translation="MENREEFLVAIARKLGRPVRRIPEAMPEPVSTLATTRLTELTMD
                     QRCEAFIKFASEVMLAKCVLVSPEDAPSKALDICWTFGSGPVIISNDQRLFDTGIAPL
                     LQKEMGAVLWTPEKGEENIHLAEKAKTGVVFAEYGLTESGGVVLFSAPERGRSVSLLP
                     EASVFVLRKSTILPRVAQLAQKLHDMAQEGQRMPSCINLIGGPSSTADIELIKVVGVH
                     GPVHATYLIIDDC"
     gene            complement(3550..6666)
                     /locus_tag="ACHKIR_004696"
     CDS             complement(3550..6666)
                     /locus_tag="ACHKIR_004696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HFO9992337.1"
                     /translation="MILQTHTIAETSNFIVLDKYIKAEQTGDSYQSESDLERELIQDL
                     QNQGYEFISVKSQSAMLANVREQLQSLNGVVFNDSEWRRFTEQYLDNPGDGILDKTRK
                     IHIDYICDFIFDNGRLENIYLIDKKNLMRNKVQIIRQFEQAGSHSNRYDVTILVNGLP
                     LVQIELKKRGVAIREAFNQIHRYSKESFNSENSLFKYLQLFVISNGTDTRYFANTTKR
                     DKNSFDFTMNWAKSDNTLIKDLKDFTATFFQKHTLLNVLVNYSVFDSSQTLLVMRPYQ
                     IAATERILWKIKSSFTAKNWSKPESGGYIWHTTGSGKTLTSFKAARLATELDFIDKVF
                     FVVDRKDLDYQTMKEYQRFSPDSVNGSENTAGLKRNLDKDDNKIIVTTIQKLNNLMKA
                     ESDLPVYNQQVVFIFDECHRSQFGEAQKNLKKKFRRYYQFGFTGTPVFPENASGSETT
                     ASVFGRELHSYVITDAIRDEKVLKFKVDYNDVRPQFKSLETETDEKKLSAAENQQAFL
                     HPMRIQEITQYILNNFRQKTHRTFPGSRGFNAMLAVSSVDAAKVYYATFKRLQEEAAN
                     KSANYKPLRIATIFSFAANEEQNAIGEISDETFDTGAMDSSAKEFLDAAIREYNDHFQ
                     MNFSTDSKDFQNYYRDLAQRVKNQDVDLLIVVGMFLTGFDAPTLNTLFVDKNLRFHGL
                     MQAFSRTNRIYDATKTFGNIVTFRNLEQATIDAITLFGDKNTRNVVLEKSYAEYMEGF
                     TDAATGEAKRGFMAVVSELEQRFPAPASIESEKEKKDFVKLFGEYLRAENVLQNYDEF
                     ATLKALQQIDLSDPVAVEKFKAEHYVDDEKFAELQMIRLPAERKIQDYRSAYNDIRDW
                     QHREKEADKKEKSTTDWDDVVFEVDLLKSQEINLDYILGLIFEHSRQNKSKDEMTVEV
                     KRLIRSSLGNRAKEGLVVDFIQQTNLDDLPDKASIIDTFFTFAQREQQREADALIKEE
                     NLNEEAARRYIRTSLKREYATENGTELNETLPKLSPLNPQYKTKKQTVFQKISAFIEK
                     FKGVGGHL"
     gene            complement(6786..7980)
                     /locus_tag="ACHKIR_004697"
                     /pseudo
     CDS             complement(6786..7980)
                     /locus_tag="ACHKIR_004697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148413.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
     gene            complement(7977..9533)
                     /locus_tag="ACHKIR_004698"
     CDS             complement(7977..9533)
                     /locus_tag="ACHKIR_004698"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFO9992338.1"
                     /translation="MTSIQQRAELHRQIWQIANDVRGSVDGWDFKQYVLGALFYRFIS
                     ENFSGYMEAGDDSICYAALDDGIITDDIKDDAIRTKGYFIYPSQLFCNVAAKANTNDR
                     LNADLNSIFVAIESSAYGYPSEADIKGLFADFDTTSNRLGNTVKDKNARLAAVLKGVE
                     GLNLGDFNKHQIDLFGDAYEFLISNYAANAGKSGGEFFTPQHVSKLIAQLAMHGQTHV
                     NKIYDPAAGSGSLLLQAKKQFDDHIIEEGFFGQEINHTTYNLARMNMFLHNINYDKFD
                     IKLGNTLTEPHFRDEKPFDAIVSNPPYSVKWTGSDDPTLINDERFAPAGVLAPKSKAD
                     FAFVLHALNYLSARGRAAIVCFPGIFYRGGAEQKIRQYLVDNNYVETVISLAPNLFFG
                     TTIAVNILVLSKHKTDTRVQFIDASELFKKEANNNILTDAHIEQIMQVFASKEDVAHL
                     AKSVAFETVVANDYNLSVSSYVEAKDTREIINIAELNAELKTTVSKIDQLRKDIDAIV
                     AEIEGCGVQK"
BASE COUNT         2532 a         2156 c         2272 g         2802 t
ORIGIN      
        1 atgcctgtta aatgtatttg tcagtacggt aaaacagctc aaaaactcca ttattgccat
       61 ttctgcgatt gttgctatgg caacggtaat ggatgtttgt gggttgattg gcactctggc
      121 acagagcctt gtagatctca ctggtggggc ctacctcttc attgctccgg ttatcggcgc
      181 tcttggtaca tttgttactg gcagtgacac aaactccaac attctgtttg gtaaattgca
      241 gaccactgcg gctgaaaaat tgcatgtgga cccactctgg ctggctgccg ctaacacatc
      301 tggcgcaaca ggcggcaaaa tgatttctcc tcagagtatt gctatcgctg tttcagccac
      361 ccgtatggat ggtcagggga gcgtaatcat gtcagggaca ctgaagtact gctttgctta
      421 tattgttatt cttggactga aagtaggcct tgtctaccac ctgttcatgg cctgagaaaa
      481 aataacaaac ggacgtggaa tattaatcaa caggagaaat atctgtgaat gttaactttt
      541 tcgtcacctg cattggtgat gcatttaaat cccgtatggc acaaaacagc gtgctgttac
      601 tggagcaatt gggttgcaat gtccatttcc ccgaaaaaca gggatgttgc ggtcagccag
      661 cgataaatgg cggttatgtt cgtgatgcca ttcctggtat gaaaaatctc atatcttcgc
      721 tggaagagaa tgactggccg gtagtatctc ctgccgggtc atgcacttac acgataaaaa
      781 attacgctcg ctatctcagt gaagaacctg agtgggcggc acgagctgaa aaagtagctg
      841 aacgcctgca tgatttgacg tcatttatag taaatcatct tggcatcaca gatgttgggg
      901 caagactcaa tggtcgcgca gtgtaccatc cttcatgcag cctttcccgt aaactgggtg
      961 taacccgaga accgcttttg ttgcttgaga atgtacgggg acttgagctg ctgccgtttc
     1021 acgctcagga aacgtgctgc gggtttggcg gcacgttctc tgtaaaaatg gcggagatct
     1081 ctggtgaaat ggtgaaagag aaagtcgggc atattatgga tgttaaacct gattttctta
     1141 tcggggctga cgccagttgc ctgctcaaca tcggcggtcg gcttagccgt gaagggcatc
     1201 ctgtaaaagt gatgcatatt gctgaagtcc tgatgcacag atgaggtaaa cagaatgtct
     1261 ttgaaaacaa gtgatatcag gtttaaagag cgtatccgta ttcagattca ggacccgatc
     1321 atgcgaaaag ctgtggctaa cgcacaggag cgcataggga ctaatcgaca gaaaatggtt
     1381 gatgaactgg ggcactggga agaatggcgt tcacttgctg aacagattcg tcggcatgtt
     1441 ctggaaaatc ttgatgctta tttgtatcaa ttgtccgaaa aagttactgc gaatggcgga
     1501 catgtatatt ttgcccgtac cagagaggag gcaaccagtt acatactgaa ggtggcgaaa
     1561 gaaaaaaacg ccaggaaggt tgttaaatcc aaatcaatgg tgactgagga aattggtatg
     1621 aacggcgttc ttcagaaagc cgggatacca gtgatagaaa cagatcttgg tgaatatatt
     1681 cttcagctgg cgcatgagcc gccatcgcat gttgttgtgc cggcaataca taaagatcgc
     1741 catcaaattc gcagagtttt gcatgaacat ctggggtatg acggccctga aactccagaa
     1801 gcaatgacat tgttcatccg tgagaaaatc agacaggatt ttctgagtgc tgaagttggc
     1861 gtaacagggt gtaactttgc cgttgcagaa acaggctcag tttgccttgt gacaaacgaa
     1921 ggtaacgcac gtatgtgcac cacgttacct aaaacacata ttgctgtgat gggtatggag
     1981 cgtattgcac caacattccg ggaggtggat gttcttatca ccatgcttgc aaggagtgct
     2041 gtgggagcaa gactgacagg ttataacacc tggcttacag gtccaagaga agaaggacat
     2101 gttgacggcc cggaagagtt ccatctcgtc attgttgaca atgggcgttc agaggttctc
     2161 ggctcagaat tcagcgatgt attgcgctgt attcgctgcg gtgcctgtat taatacctgt
     2221 cccgcttatc gccagattgg tggacatggc tatggctcaa tctacccggg cccaatcgga
     2281 gcagttattt ccccgttgct tggtggatat gaagacttta aggaattacc ttatgcctgc
     2341 tcattgtgca ctgcctgcaa tggggtatgt ccggtaaaaa tacctctggc acaactaata
     2401 caaaaacatc ggcaggttat ggcagagtcc gaaataacac catcaacaga acgccgtata
     2461 accagaatat tcaattacgt aaacagtcat ccggcattgt ggaaagttgg catgattgct
     2521 ggcgcgaaaa cagccagcct gtgcatcagg aacggaaaaa cgccgattag cccaggtgct
     2581 attaaagaat ggtctgtggc cagagactta ccttctcctg atggagaaag cttccgtagt
     2641 tggttcaaaa gacataaaaa aggagggtaa ctttatggaa aacagagagg aatttctcgt
     2701 tgctatcgcc agaaagcttg gcaggccagt aagacgtatt cccgaagcaa tgccggagcc
     2761 tgtaagtacg ctggctacga cacgtttaac cgagttgaca atggaccaac gctgtgaggc
     2821 ttttattaaa tttgcctcag aagtgatgct tgcgaagtgc gttctggttt cgcctgaaga
     2881 cgcaccatca aaagcattag acatctgttg gacgttcggt tccgggccag tcattatcag
     2941 caacgatcaa cgactgtttg atacggggat tgcaccattg ctacaaaaag agatgggcgc
     3001 tgtcctgtgg accccggaga agggtgagga aaatattcat cttgcagaaa aagcaaaaac
     3061 aggcgtcgtt ttcgctgagt atggactgac agaatctggt ggtgttgttt tattctccgc
     3121 ccctgaacga gggcgttcag taagtctttt acctgaagcg tctgtgtttg ttttacgcaa
     3181 aagtaccatt cttcccagag tggctcagct tgcgcaaaaa cttcatgata tggctcagga
     3241 aggacaacgt atgccttcct gtatcaatct gattggaggc ccaagttcta ctgcagatat
     3301 agaactgatc aaagttgttg gtgtgcatgg acctgtacat gcaacttact tgattattga
     3361 tgactgctaa tacattttag tatgtatata tgattaacag ctccgtttag ctgagctgtt
     3421 aatcattcat ttagttgtat tagtcgtgac ttgagcgtaa gcgtcaatac ggactccgtt
     3481 aatgccttac aatagcagtc agtgttcatg cctgtttatt gaggcaaggg ggagtccggg
     3541 cgcctgtaac taaagatgcc cccccacgcc tttaaacttc tcaataaatg cgctaatctt
     3601 ctgaaaaacg gtctgttttt tcgttttata ttgcgggttt aacggactaa gttttggtaa
     3661 tgtctcgttt aattctgtgc cattttcggt ggcgtattcg cgttttaacg acgtgcgaat
     3721 atagcgcctg gccgcttctt cattaagatt ttcttctttg atcaatgcat ctgcttcacg
     3781 ttgttgttcg cgttgagcaa aggtaaagaa cgtatcaatg atgctggctt tgtccggcaa
     3841 atcatcaagg ttcgtttgct gaataaaatc gaccaccagg ccttctttag cacggttccc
     3901 cagacttgaa cgaattaatc gtttgacctc tacggtcatt tcgtccttac ttttgttttg
     3961 tctgctgtgt tcgaaaatca ggccaaggat ataatccagg tttatttcct gagacttcag
     4021 caaatcgacc tcaaaaacca cgtcatccca gtcagtggtt gatttttctt ttttgtcagc
     4081 ttctttttcg cggtgctgcc agtcacgaat atcgttatag gcagaacgat aatcctgaat
     4141 cttgcgttca gcagggagac gaatcatttg caattcagcg aacttttcat catccacata
     4201 atgttctgct ttgaattttt ctaccgcaac aggatcgctg agatcgattt gttgcagggc
     4261 tttcagcgtg gcaaattcat catagttttg caggacgttc tcggcacgca ggtattcacc
     4321 aaacagttta acgaagtctt ttttctcttt ttcgctttca atactggcag gggcagggaa
     4381 cctttgttcc agttctgaaa ctactgccat aaagccgcgt ttagcttcgc cagtggcagc
     4441 atctgtaaag ccttccatat actctgcata actcttttcc agcaccacat ttctggtgtt
     4501 tttatcacca aaaagtgtga tggcatcgat ggttgcctgc tccaggttac ggaatgtgac
     4561 gatgttaccg aaggttttcg tggcgtcata aatgcggttg gtacgggaaa acgcctgcat
     4621 cagaccgtga aaacgcaggt ttttatcgac gaacagcgtg ttcagtgttg gagcatcgaa
     4681 gccggttaaa aacatcccca cgacgattaa caggtcaaca tcctgatttt taacccgctg
     4741 agccaggtcc cgatagtagt tctgaaaatc cttgctatca gtactgaaat tcatttgaaa
     4801 atggtcgtta tattcacgaa ttgcagcatc cagaaactct ttagcactgc tgtccattgc
     4861 gccggtatca aaagtttcat cggaaatttc accaatggca ttttgttctt cattggcggc
     4921 aaaggagaat attgtcgcaa tacgcagtgg tttataattt gcagatttat tagcggcttc
     4981 ctcctgtaag cgtttaaacg tcgcgtaata gactttcgca gcatccacgc tgcttactgc
     5041 cagcatggca ttaaaacctc tggaacccgg gaaagtgcgg tgggtcttct ggcggaagtt
     5101 attcaggata tattgcgtga tttcctgaat acgcatggga tgaagaaacg cctgctgatt
     5161 ttcagccgca ctcagttttt tctcgtcagt ttctgtctct aaagacttaa actgtggccg
     5221 cacatcgttg tagtccacct tgaatttgag cactttttcg tcacgaatcg catcggtaat
     5281 cacatacgaa tgcaattcac gaccaaatac gctggcggtc gtttctgaac ctgaggcgtt
     5341 ttccgggaaa acaggtgtgc cggtaaaacc aaactgataa tagcgtctga atttcttctt
     5401 cagatttttc tgcgcttctc caaactggct gcggtggcat tcatcaaata taaacaccac
     5461 ttgctgattg tatacaggca ggtcgctttc tgctttcatc aggttattaa gtttctgaat
     5521 agtggtgacg ataattttgt tatcgtcctt atccagattt cgtttaaggc ctgcggtatt
     5581 ttccgagccg ttaacactat ctggcgaaaa acgctgatat tccttcatgg tctggtaatc
     5641 gaggtctttc ctgtcgacca caaagaagac tttatcaata aagtccagtt ctgttgccag
     5701 acgcgcggct ttaaagctgg tcagtgtttt accggaaccc gtggtgtgcc agatataccc
     5761 accgctttcc ggttttgacc agttcttcgc tgtaaaggag ctcttaattt tccacagaat
     5821 gcgctcggtg gcagcaatct ggtacggtcg catcaccagt agcgtctgac tgctgtcaaa
     5881 aacgctgtag ttcaccagaa cattaagcag ggtatgtttc tggaaaaagg tggcggtaaa
     5941 atctttgagg tctttaatca gcgtgttgtc tgatttcgcc cagttcatgg tgaagtcaaa
     6001 actgttttta tcgcgctttg tcgtgttggc aaagtaacgg gtatcggtac cgttggaaat
     6061 gacgaacagt tgcagatact taaacaggga attttcgctg ttaaaactct ccttgctgta
     6121 acgatgtatc tggttgaaag cctcacgaat cgccacgcca cgctttttca gttcgatttg
     6181 caccagcggc aaaccattaa ccaggatagt gacgtcataa cggttactgt gagaacctgc
     6241 ctgttcaaac tgccggataa tctgcacttt gttgcgcatg agattctttt tatctatcag
     6301 atagatattc tcaagacgcc cgttgtcaaa aataaagtca cagatatagt cgatatggat
     6361 tttacgggtc ttatccagaa tgccatcgcc agggttgtcc agatactgct ccgtgaaacg
     6421 ccgccattcg ctgtcattaa acaccacgcc attgaggctc tgaagctgtt cccggacatt
     6481 agccagcatc gccgactgtg attttacgga aataaattca tagccctgat tctgcaggtc
     6541 ctgaatcagt tcacgttcca ggtccgattc actctggtag ctgtcgcctg tttgctcagc
     6601 cttgatgtac ttatcaagaa cgataaagtt actggtttca gcaatggtgt gtgtctgaag
     6661 aatcatagcg catcctttgt gccgtctggc ggggccggaa gggagttaag ggtgacttcc
     6721 ggcgtgtaaa aatggtttat atactgaccg gatgttaagg tggcccggtc ggtagcaatt
     6781 gtcaattagt tactgacggt ttcaggtttc gggaaactga acagtaaatc acgatagtac
     6841 tcgtattgtt tctggcgcaa ctcgatttca cgcgggagac cttcggtgat ggagttggtc
     6901 agggtgtcga atttatcgag tatttcgaca atgcgagctt gttcttctga aggtggtaca
     6961 ggtattggga attttaaaaa cattggtcga cgaagtgagg taaccgatga acttactgat
     7021 gctttttgaa tataggtaaa aaaatatgct gttatatagt gatatacaaa tcgcggattc
     7081 ataaatgcat ttgggacaat acgatatgct ctctgatgca aagcatattt acctttagcg
     7141 taatgaaata ccttccccac cccggcacca tctccagccg taatgattgc tgtttcatca
     7201 aaatcaaata catttaactt taatggttca cgtccacgtg cataaaaaat atatttgcca
     7261 tgttctatag catcttgcgt atcatggtta cctgttccta taacagccac ttcccccaaa
     7321 gtcttccact caatccgaat accatccatg gcatccggaa ggatgtaatt accccccccc
     7381 gcgcaatatc tttgaaattc aataacttat ctagatagtg gttgtattgt tttttacgca
     7441 tgttaagctc tgctgtcagc tctgctgtca gggcgctgaa tttgtccaga attcgcacga
     7501 tttcagactg gatggcaagg gatttttccg gattatccgg gcaggggatt ggtacaggta
     7561 tattttcaac ctgatgactc attaacttgg gattcccatt cctgagtaaa catgtttttt
     7621 ggcctctatt gttagccagt aaaacagaaa tttgtagatt aatggatatt tactttttaa
     7681 tgtaattagg ccgcaaacat tagttattga aaatttacct gttcgataga acacagtgcc
     7741 tgcatttgcg ccatctgttg tccagcttat atattcgcca tcaaaatcga aggtattgat
     7801 agaacctatt ttcccattat ttgcagtttg agagctatag acaggatatg ggccaaaatt
     7861 ttcagttaaa tatccctttg acataactct gcctcttctt aaggctgaga cttcccccaa
     7921 tggcaaccac tcaacctcaa ccccatccag caatttttcc agataactca actcgctcat
     7981 ttctgcaccc cgcagccttc aatttcagcc acaatcgcat caatatcttt acgcaactgg
     8041 tcgattttgc tgaccgtggt tttcagctca gcattcagct cagcaatatt gataatttca
     8101 cgagtatctt tcgcttccac atagctgctc accgacaggt tatagtcatt agcgacaacg
     8161 gtctcaaacg cgactgattt cgccagatgg gcaacatctt ccttgctggc aaatacctgc
     8221 ataatctgtt cgatatgagc atcggtcaga atgttgttgt tagcctcttt tttgaacagt
     8281 tcgctggcat caataaactg aactctggta tccgttttat gtttagacag caccagtatg
     8341 tttacggcaa tggtggtgcc aaagaacagg ttcggtgcca gtgaaatcac ggtttcgaca
     8401 tagttattgt cgaccagata ctgacggatt ttctgctctg cgccgccacg gtaaaaaata
     8461 cccgggaagc agacaatcgc agcgcgacct ctggccgaaa gatagttcag cgcatgtaat
     8521 acaaacgcaa agtcagcttt ggatttgggg gccagaacgc cagccggggc aaaacgctca
     8581 tcgttaatca gtgtcgggtc atcgctgcca gtccatttca ccgaatacgg cgggttagaa
     8641 acgatggcat caaacggttt ttcatctctg aagtgcggct cagtcagcgt attgcccagc
     8701 ttgatatcaa acttgtcgta gttgatgttg tgcaaaaaca tgttcatacg cgccaggtta
     8761 taggtcgtat ggttgatttc ctgaccaaaa aagccttctt cgatgatatg gtcatcaaac
     8821 tgctttttcg cctgcaacaa cagcgaaccg gaacccgctg ccgggtcgta gattttgtta
     8881 acgtgggtct gcccgtgcat agccagttgt gcaatcagct tagagacgtg ctgtggtgta
     8941 aagaactcgc cgcctgactt accggcattt gcggcatagt tagaaataag gaattcatag
     9001 gcatcgccaa acaggtcaat ctgatgtttg ttgaagtcac caaggtttaa cccttcaacc
     9061 cctttcagaa cagcagccag gcgggcattt ttatccttaa cggtgttacc caggcggtta
     9121 ctggtggtat cgaaatcagc aaacaaaccc ttgatgtcag cttctgaagg ataaccgtaa
     9181 gcagaacttt cgatagcaac gaagatgctg tttaaatctg cattcagtct gtcattggta
     9241 tttgctttcg cagctacgtt gcagaaaagc tgactggggt agatgaagta gcctttagtc
     9301 ctgatggcat cgtctttaat gtcatcagta ataatgccgt catcaagtgc cgcataacag
     9361 atgctgtcat caccggcttc catatacccg gagaaatttt cgctgataaa acggtagaaa
     9421 agcgcgccca gaacgtattg cttaaaatcc catccatcga ccgaacctct gacatcgtta
     9481 gcaatttgcc atatctggcg gtgcagttct gctctttgtt gaatacttgt catttttaac
     9541 ctgtttttcc tggtctggaa ctgaatctcc gggggcaata ctacagcaat cttgtggttc
     9601 aggcagcccc ggagcaagtc agcagatatt gtgagataca ccaggttatt catgacaggg
     9661 aggaggaacg gaacactgcc tttcttttac agtgcaccgg gtacattcaa aataatttca
     9721 gacagatatt atcgttgtga gtcccccgcc acctttccgg tg
//
LOCUS       DAZGQL010000152         9750 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.152, whole genome shotgun sequence.
ACCESSION   DAZGQL010000152 DAZGQL010000000
VERSION     DAZGQL010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9750
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.152"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..173)
                     /locus_tag="ACHKIR_004699"
     CDS             complement(<1..173)
                     /locus_tag="ACHKIR_004699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992339.1"
                     /translation="MAGNFADLTAVLTLDSARFSEEAARVKKELGETSALADLMSGKV
                     SQSFRKQADAAEQ"
     gene            complement(223..525)
                     /locus_tag="ACHKIR_004700"
                     /pseudo
     CDS             complement(223..525)
                     /locus_tag="ACHKIR_004700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309140.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
     gene            complement(552..974)
                     /locus_tag="ACHKIR_004701"
     CDS             complement(552..974)
                     /locus_tag="ACHKIR_004701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFO9992340.1"
                     /translation="MFLKTEQFEYNGVSVTLSELSALQRFDYMKFVSDAAQQETTEHD
                     AVHINQRYLETASLLVAMSLWHSHSLKGTLASPETEMQQIRREVMLGWPADALNQATN
                     RVLYLSGMLDNRHDADPEPTGKAEATEPVTSKKHSKAS"
     gene            complement(991..1740)
                     /locus_tag="ACHKIR_004702"
     CDS             complement(991..1740)
                     /locus_tag="ACHKIR_004702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9992341.1"
                     /translation="MTLPNPLAPVKGSGTTLWVYTGNGDAWANPLSDDDWTRLAKIKD
                     LTPGEMTAESYDDNYLDDEDADWVSTGQGQKSAGDTSFTLAWKPGEKGQRDLIAWFDS
                     SETRAYKIRFPNGTVDVFRGWVSAIGKSVTAKEVITRTVKITNIGRPSLAEDRGEITP
                     VTGITVTPLTGNVAKGQNITLTVAVQPEGATDKTFRATSANQNFATITVKGNTIIVKG
                     VAAGKAQIPVVTGNGEFAAVAEITVTDGAAG"
     gene            complement(1748..2146)
                     /gene="gpU"
                     /locus_tag="ACHKIR_004703"
     CDS             complement(1748..2146)
                     /gene="gpU"
                     /locus_tag="ACHKIR_004703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail terminator protein"
                     /protein_id="HFO9992342.1"
                     /translation="MKHREIRAAVLSALKENISERVNWFDGRPVFIDEQELPAVAVYL
                     TDASATEEFVDEGTWEAVLHIEVFLRAKETDSVLDMWMEEKILPALEAVPGLSALLLK
                     MNLQGYDYRRDDEYMMWGSADLQWNITYQM"
     gene            complement(2159..2782)
                     /locus_tag="ACHKIR_004704"
     CDS             complement(2159..2782)
                     /locus_tag="ACHKIR_004704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9992343.1"
                     /translation="MAIKGLAQAMKNLDAIDRRAVPRASATTLNRVAGAIIAKTASSV
                     ARELAVPRRLIRARIRLSPARPDKVYAKVYINTGNLPAIKLGEARVRLSRRKRRKKGQ
                     RAALKGGGSVLIVGKRRIPDAFITRLANGRWHVMQRMPWAPSSSGADSKGRPKRHRLP
                     IEVVKIPTAGPLAETFERERDRMYREKLPAQMMTAMAHQLRLVLKRK"
     gene            complement(2785..3066)
                     /locus_tag="ACHKIR_004705"
     CDS             complement(2785..3066)
                     /locus_tag="ACHKIR_004705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA breaking-rejoining protein"
                     /protein_id="HFO9992344.1"
                     /translation="MSDLFTRMCCRMDGATVRVMGKQAEINGVVYDVMPEEESAEMGA
                     LSGSQLSLVVFSARYRPARHDVVVFAGRTLTVTRYDTYNGKPRIFVEQE"
     gene            complement(3059..3385)
                     /locus_tag="ACHKIR_004706"
     CDS             complement(3059..3385)
                     /locus_tag="ACHKIR_004706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid cement protein"
                     /protein_id="HFO9992345.1"
                     /translation="MAKNFVQDGTTIELVNAGDQTILSGAAVVVGSMVAVAITDIPAG
                     DAGDGFAEGVFLLPKQSADDIQSGAVVYLKDGVVQLAADGAVAAGVAWENAPANSATV
                     AVKINV"
     gene            complement(3473..5452)
                     /locus_tag="ACHKIR_004707"
     CDS             complement(3473..5452)
                     /locus_tag="ACHKIR_004707"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001369631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpP-like prohead protease/major capsid protein
                     fusion protein"
                     /protein_id="HFO9992346.1"
                     /translation="MKNKSWFRMQAGGPGDADIYIYDEIGFWGVTAKQFVSELNALGD
                     ITHINLHINSPGGDVFEGIAIFNALKNQGATITVYVDGVAASMASVIAMAGDTVIMPE
                     NAFMMIHKPWGFSGGDAEDMRSYADLLDKVESVLLPAYAQKTGKTTDEIAAMLADETW
                     MSGAECLAHGFADQVTPAVEAMACIQSKRTEEFKKMPESIRNMITPPRNSAPRDTTVT
                     IPAPAVTEPSPVPAVSDEATIRARVMAEQKARMSGINDLFAMFGGRYQTLQAQCVADP
                     DCSLEMARERLLNEMGKESSPTNKNTPAHIYAGNGNFVGDGIRQAMLARAGFENVEKD
                     NAYNGMTLREWARMSLTERGIGVASYNPMQMVGLALTHSTSDFGNILLDVSNKGLIQG
                     WEESEETFQKWTRKGRLSDFKTAYRVGMGGFGSLRQVREGAEYKYITTSDRKETIALA
                     TYGEIFSITRQAIINDDLNMLVDVPMKMGRAAKATIGDLVYKVLTDNPKLSDGKALFH
                     ADHKNIATGGISVSGLDAARQMMRLQKEGERALNIRPAFMLVPVALETVANQTIKSAS
                     VKGADANAGVINPIQNFAEVIAEARLDAADPKTWYLAAAQGTDTIEVAWLDGVDTPYI
                     DQQEGFTTDGIATKIRIDAGVAPLDWRGLVRSSVA"
     gene            complement(5442..6944)
                     /locus_tag="ACHKIR_004708"
     CDS             complement(5442..6944)
                     /locus_tag="ACHKIR_004708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO9992347.1"
                     /translation="MAIIDDVIGVFSPGWKAARLRSRALIMAYEAVKPTRTHKARREN
                     RSADQLSKYGAVSLREQARFLDINHDLVIGVFDKLEERVIGARGIIVEPQPLRKNGEM
                     VAELAADIRRLWAEWSVSPDVTGQYTRPVLERLLLRTWLRDGEVFAQMVSGAGNGLER
                     TAGVPFWLEAMEPDFVPMRTDESAGLNQGVFLDEWGRPKKYLVYKNYPVSGRQSDTKE
                     IAAGKMIHLKFTRRLHQTRGSSMLSGVLMRISALKEYEDAELTAARIAAALGLYIRKG
                     DGQDYEDPGIKETEREVHITPGIIYDDLRKGEDIGMVKSDRPNPNLETFRNGQLRAVA
                     AGSRLSFSSAARNYNGTYSAQRQELVESTDGYLILQDCFIGAVTRPVYRTWLNMVVAA
                     GLLKIPADVEMKTLYNATYSGPVMPWIDPVKEAEAWRIQIRGGAATESDWVRAGGRNP
                     DEVKRRRKAEIDENSRLGLVFDTDPVNDKGGNSAGTERQYQRDTESQHEE"
     gene            complement(6944..7156)
                     /locus_tag="ACHKIR_004709"
     CDS             complement(6944..7156)
                     /locus_tag="ACHKIR_004709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992348.1"
                     /translation="MSTEEAREMIQRYREAEMAVLEGKSVTFNGQQLTLESLSQIRAG
                     RQEWERRLAAMVSRRRGKPGFKLARF"
     gene            complement(7153..9276)
                     /locus_tag="ACHKIR_004710"
     CDS             complement(7153..9276)
                     /locus_tag="ACHKIR_004710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFO9992349.1"
                     /translation="MNQVNESHSRASDIWREVASLFRPPGRLPVAEAIRRYMRVPRGA
                     NTSGPWESSLTPYMIDPINTLSAREYDAVVFVGPARTGKTEGLIDGWIVYGIICDPAD
                     MLVVQMTETKAREHSRTRLSRTFRHSPEVCKRLSPSRNDNNVHDKMFLDGSFLKIGWP
                     SITVFSSSDYRRVALTDYDRFPENVDGEGDAFTLASKRTTTFMSSGMTLVESSPGRDI
                     TDTKWRCGGAHEAPPTTGILSLYNRGDRRRWYWPCPHCGEYFQPVMDNMTGYRNNPDF
                     VAAGQAARLMCPHCRGLIAPEQKRELNNQGIWLREGERAAADGSITGTPRNSRIASFW
                     MEGPAAAFQTWEQLIFKLLAAEEEYERTGSEETLKAVVNTDIGRPYLPRSATEQRKSE
                     LLEQRAEPFPRRSVPDGVRFIEATVDVQGGKNRRFVVQITGYGEQGERWIVDRYNIRH
                     SLRCSPNGESLPVDPAAYPEDWDLLLTDVFHKTWPLASDPDVRMRLMAMAVDTGGEAG
                     VTDNAYRFWRRCRSDGLGNRVFLFKGDGLRRDRLINRTFPDNTGRSARRARASGDVAL
                     WLVQTDAFKDRVNNALWRDTPGPNYIHFPDWLGRWFYDELTYEERGSDGKWRKPGRGA
                     NEAFDLLVYADALAVLHGYEKIRWPSAPDWAQRETWLVFPQARSGETVSPELTAGAEK
                     RRRRKKKLRTERAEDNPWITSGGWL"
     gene            complement(9273..9749)
                     /locus_tag="ACHKIR_004711"
     CDS             complement(9273..9749)
                     /locus_tag="ACHKIR_004711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HFO9992350.1"
                     /translation="MDRELKNLHLNISQLAALSGAHRQTVAARVKNISPAGGHESNLK
                     LYRLTDILAELMKAPLPVDNEEMDPHARKAWYQSERDRLKFEQETGLLVPVSDVRRSF
                     SVVVKAIVQVLETWPDRLERDRGWTASQLNEVQIVVDEIRDTLEKAVIDCCDEADM"
BASE COUNT         2060 a         3063 c         2357 g         2270 t
ORIGIN      
        1 ctctgctcag cagcatcagc ctgttttctg aaagactgac tgactttccc ggacatcaaa
       61 tcagcaagcg cactggtttc acccagctct ttttttaccc gcgctgcctc ttcagaaaaa
      121 cgggcagaat ccagtgtcag cacggctgtc agatcggcaa aattacctgc catcgtggtc
      181 acctcctgaa atgtcctctg atgccatcaa taactgtcac aacctccttc cctcctcccc
      241 gaaacggact ccaccggcga ggcccgccgc cttctgcatc agtacatcat tttcgtccgg
      301 cgtctccgcc tgcccttcct ctgcctccgg agcgaacagg ctgaaatccg ccggatgcat
      361 atccggatcg caaaaaaaca ggctgagtac ggcgtacgtc agcccggaaa aatgcatatc
      421 cagctgggta tcctgaaaat aatgcgtgca gtaaaaacgt cgccagtcgg catattcggt
      481 ggatgtcatc ccggcaagca tggcgcgcca gtcggctctc cccatctcac gcgccagttt
      541 caggacaaag ttcagctcgc cttcgaatgc ttttttgatg ttaccggctc agtcgcttct
      601 gctttcccgg ttggttcagg atcggcatcg tgccggttat ccagcatacc tgaaagataa
      661 agcacccggt tcgttgcctg attcagtgca tcagcaggcc atcccagcat cacttcacgg
      721 cggatctgct gcatctctgt ctccggagat gccagagtgc ctttgaggga atgggaatgc
      781 catagcgaca tcgccacaag cagggatgcc gtttccagat atcgctggtt aatgtgcacg
      841 gcatcatgct ccgttgtctc ctgttgtgct gcgtctgaaa caaacttcat ataatcaaac
      901 cgctgcagcg cagacagctc cgacagcgtg acggacaccc cgttatattc aaattgttct
      961 gttttcagga acatgcttga tctcctcccc tcagcccgca gcgccatccg tgacggtgat
     1021 ctccgccacc gccgcaaact caccgttgcc ggtgacaaca gggatctgcg ctttacctgc
     1081 cgcaacacct ttcacgatga tcgtgttccc tttcacggta atggtggcga aattctgatt
     1141 cgctgaggtt gcgcggaacg ttttatccgt tgcgccttcc ggctgaacgg ccacggtcag
     1201 ggtgatattc tgaccttttg ccacattccc cgttagtggc gtcacggtaa taccggtgac
     1261 cggtgtgatt tctccccgat cctcagccag cgacggacga ccgatattgg tgattttcac
     1321 agtgcgggtg atcacttctt tggcggtcac ggatttacca atggcgctca cccagccacg
     1381 gaacacatcc accgtgccgt tcgggaagcg gattttgtag gcccgggtct cgctgctgtc
     1441 aaaccaggca atcaaatcac gctgcccttt ctcgccgggc ttccaggcca gcgtaaaact
     1501 ggtgtcaccg gcagatttct gcccctgccc ggtggatacc cagtcagcat cctcatcatc
     1561 cagatagtta tcgtcgtagg attccgccgt catctcgccg ggggtcagat cctttatttt
     1621 tgccaggcgc gtccagtcat catctgacag cgggtttgcc caggcatcgc catttccggt
     1681 ataaacccag agcgtggtgc cggaaccttt taccggcgca aggggattag ggagcgtcat
     1741 aacgtcctca catctggtaa gtaatattcc actggagatc agccgatccc cacatcatat
     1801 attcgtcatc ccggcgatag tcatacccct gaagattcat cttcagcagt aatgcactta
     1861 gcccgggaac cgcctccagc gccggaagta ttttttcttc catccacata tccagcacgg
     1921 agtccgtctc tttcgccctg agaaacacct caatatgaag cacggcctcc caggtcccct
     1981 cgtcaacaaa ctcctccgtc gcagaagcat ctgtcagata aaccgccacc gcaggcagtt
     2041 cctgttcatc aataaatacc gggcggccgt caaaccagtt cacccgctca gaaatatttt
     2101 ctttcagggc agacagaact gccgcccgta tttcacggtg tttcatacac cctccctgtc
     2161 attttcgttt cagcaccagg cgtaactgat gcgccatggc cgtcatcatc tgcgccggta
     2221 atttttcccg gtacatccgg tcccgttcac gttcaaaggt ttctgccagc ggtccggcag
     2281 tcggaatctt caccacttcg atcggcagac ggtggcgttt cggcctccct ttgctgtccg
     2341 cgccgctgga cgatggtgcc cacggcatac gctgcatcac atgccagcgt ccgttagcca
     2401 gccgggtgat aaaggcgtcc gggatccgtc ttttccccac aatcagcaca ctgccgcccc
     2461 ctttcagggc cgcacgctgt cctttctttc tccgttttct gcgggaaagt cgaacgcggg
     2521 cctcccccag tttgatggcg ggcaggttgc cggtattgat gtaaaccttt gcgtaaacct
     2581 tatccggtcg tgccggactt aaccggatgc gggcacggat aagacgacgg ggaacggcca
     2641 gctccctggc aactgaagag gccgttttcg caataatggc ccccgccacg cggttcagtg
     2701 tcgtggcaga ggcccgggga acggcacggc ggtcaattgc atccagattt ttcatggcct
     2761 gcgccagacc ttttattgcc atactcattc ctgttcgaca aaaatccggg gtttaccgtt
     2821 gtacgtgtca taacgggtca ccgtcagtgt gcgccccgca aacacaacaa catcatgacg
     2881 ggccggacgg taccgggctg aaaacaccac cagtgacaac tggctgcccg aaagcgcccc
     2941 catctccgcg gactcttcct ccggcatcac gtcatacact acgccgttaa tctccgcctg
     3001 tttgcccatc acccgaacgg tcgccccgtc catccggcaa cacattcgcg taaacagatc
     3061 agacattgat ttttaccgcc acagtggcgc tgtttgcagg agcattttcc caggctaccc
     3121 ccgcagccac cgcaccgtct gcagccagct gcacaacccc gtccttcaga taaaccaccg
     3181 cgccggactg aatatcgtca gcagactgtt tgggcagcag gaacacgcct tcggcaaaac
     3241 cgtcaccggc atcaccggca ggaatatcgg taatggccac tgccaccata ctgccgacca
     3301 ccaccgcagc accgctcagg atggtctgat ctccggcatt caccagttca atggtggtgc
     3361 cgtcctgtac aaaatttttc gccataatgc tgtttctccg gacagcccct gtggggctgt
     3421 ttttcaggca taaaaaaagc cctttcgggc agtgattgtg ataacgcggt tatcaggcca
     3481 ccgacgaacg caccagaccg cgccagtcaa gtggtgccac accggcatca atacgaattt
     3541 ttgtggcaat gccgtcagtg gtgaaacctt cctgctgatc aatgtatggc gtgtccacac
     3601 catccagcca ggccacttca atggtgtcag tgccctgtgc cgccgccaga taccaggttt
     3661 tcggatctgc cgcatcaaga cgcgcttccg caatcacctc agcaaagttc tggatggggt
     3721 taatgacacc ggcgtttgca tccgcccctt tcacactggc cgatttgatg gtctggttcg
     3781 ccaccgtctc cagtgccacc ggtaccagca taaaggccgg acggatattc agggcgcgct
     3841 cgccttcttt ctgcaggcgc atcatctgac gggccgcatc cagtccggaa acggagatcc
     3901 cgccggtggc aatatttttg tgatcggcat ggaacagcgc cttaccgtcg gacagtttcg
     3961 ggttatccgt cagcaccttg taaaccaggt caccaatcgt tgccttcgcc gcacgcccca
     4021 tcttcatcgg cacgtccacc agcatattca gatcatcatt gataatggcc tggcgggtga
     4081 tggagaaaat ctccccgtag gtggccagag caatggtctc cttgcgatct gaggtggtga
     4141 tgtatttata ctccgccccc tcacgaacct gacgcaaaga accaaaaccg cccatcccca
     4201 cgcgatacgc tgttttgaag tctgacaggc gtcccttacg ggtccacttc tggaaggttt
     4261 cttctgattc ctcccagccc tggatcagcc ccttgttcga cacatccagc aggatattgc
     4321 caaaatcaga ggtgctgtgc gtaagcgcca gcccgaccat ctgcatgggg ttataactgg
     4381 ccaccccaat accgcgctcc gtcagtgaca tacgagccca ttcacgcagg gtcatcccgt
     4441 tataggcgtt atccttctcg acattttcaa atccggcacg ggccagcatc gcctggcgga
     4501 tcccgtctcc cacaaaattg ccgtttccgg cataaatatg ggccggtgtg tttttgttgg
     4561 tcggcgagga ctccttgccc atttcattca gcagacgttc acgggccatt tccagcgaac
     4621 agtcaggatc agccacgcac tgtgcctgaa gcgtctgata gcgaccgccg aacatggcaa
     4681 acagatcgtt aatgcctgac atgcgtgctt tctgctcagc cataacgcgg gcgcgaatgg
     4741 tcgcctcatc agacactgcc ggtaccggtg atggttctgt taccgccggt gcagggattg
     4801 tcactgtggt atcacgcggg gcactgttgc gtggtggagt aatcatgttt cggatggatt
     4861 ccggcatctt tttaaattcc tctgtacgtt ttgactgaat acatgccatt gcctcaacag
     4921 cgggtgtcac ctggtcagca aatccgtgtg ccagacattc ggcaccggac atccaggttt
     4981 catccgccag catggcggca atttcatcgg tggtttttcc ggttttctgc gcataggctg
     5041 gcaacagtac cgattcgact ttatccagca aatcagcata actgcgcata tcctcagcat
     5101 ccccgccact gaatccccat ggcttatgga tcatcatgaa ggcattttcc ggcataatga
     5161 ccgtatcacc ggccatcgca atcacagatg ccatcgaggc ggcaacgcca tccacataca
     5221 cggtaatggt cgccccctga tttttcaggg cattaaaaat ggcgatgcct tcaaagacat
     5281 cgccacccgg tgagttgatg tggagattaa tgtgggtgat atcacccaga gcattcagtt
     5341 cgctgacaaa ctgcttcgcg gtaactcccc agaaaccaat ctcgtcataa atataaatat
     5401 ccgcgtcacc cggcccccca gcctgcatcc tgaaccagga tttattcttc atgctggctt
     5461 tcggtgtcac gctgatactg tcgttcagtt ccggcactgt tgcctccttt gtcgttgacg
     5521 gggtcagtat caaagaccag ccccagtctg ctgttttcat caatttcagc cttgcggcga
     5581 cgtttgacct catccggatt gcgcccgccg gcacgcaccc agtcagattc tgtcgctgca
     5641 ccaccccgga tctgaattct ccaggcttca gcttccttaa ccgggtcgat ccacggcatc
     5701 accggaccgg aatacgtcgc gttatatagc gttttcatct ccacatccgc cggaattttc
     5761 agcagacctg ccgcaaccac catattcagc catgtccggt acaccgggcg ggttaccgcg
     5821 ccaataaaac agtcctgcag gatcaggtaa ccatccgtgg actcgaccag ctcctgccgc
     5881 tgggcgctgt aggtgccgtt atagttacgc gccgcactgg aaaaactcag acgactgcca
     5941 gctgccactg cacgcaactg gccgttgcgg aaagtttcaa ggttgggatt gggacggtca
     6001 gatttgacca tgccgatatc ctcgcccttg cgcaaatcgt cataaataat acccggggtg
     6061 atatggactt cccgctcggt ttctttgatc cccggatctt catagtcctg tccgtcacct
     6121 ttacggatat acagtcccag cgccgcagca atacgcgccg ctgtcagttc cgcatcctca
     6181 tactctttaa gggcactgat ccgcatcagc acccccgata acatggatga gcctcgcgtc
     6241 tgatgcagac gacgagtgaa cttcaggtgg atcatttttc cggcagcgat ttctttcgta
     6301 tcactctgcc ggccgctgac cggataattt ttataaacca gatatttttt cggtcttccc
     6361 cactcatcaa gaaaaacccc ctgattcagc ccggcggatt catcagtgcg catgggaaca
     6421 aaatccggct ccatcgcctc aagccagaat ggcactcccg ccgtccgttc cagaccgttt
     6481 cccgcaccac tgaccatctg cgcaaacact tcaccatccc gcagccaggt ccgcagcagt
     6541 aaacgttcaa gcacgggacg ggtatactgc cctgtcacat ccggactcac ggaccattca
     6601 gcccacaacc ggcggatatc cgcagccagc tctgccacca tttccccgtt ttttcgtaat
     6661 ggctgaggct ccacaataat tcccctggca ccaatcaccc gctcttccag cttgtcaaac
     6721 acaccaatca ccaggtcatg attgatatcc agaaaacggg cctgctcccg cagggaaacc
     6781 gcaccgtatt tactgagctg atcagcagag cgattttccc gccgggcttt atgtgtccgg
     6841 gtcggtttca ccgcctcata ggccatgatt aacgcccttg aacgcagtct ggctgctttc
     6901 catccggggg aaaacacgcc tatcacatca tcaataattg ccattaaaac ctcgccagtt
     6961 taaatcccgg ttttccccgc ctgcggctca ccatcgcggc aagcctgcgt tcccactcct
     7021 gacgtccggc gcggatctga gaaaggcttt ccagcgtcag ttgctgcccg ttgaaggtga
     7081 cagacttccc ctccagtacg gccatttccg cttcacggta ccgctgtatc atttctctgg
     7141 cttcttctgt gctcacaacc agcctcctga tgttatccat ggattatctt ccgcacgctc
     7201 cgtccgcagt tttttcttcc ggcgacggcg tttttctgcc ccggccgtca gttccgggga
     7261 taccgtttca ccagaacgcg cctgcgggaa gacgagccac gtttcccgct gtgcccagtc
     7321 cggtgcggag ggccagcgga tcttttcata accatgcaga acagcaagcg catccgcata
     7381 aaccagcagg tcaaacgcct cgttagcgcc cctgcccggt tttcgccatt ttccgtcact
     7441 gccgcgctct tcataggtca gctcatcgta aaaccaccgc cccagccagt cgggaaagtg
     7501 gatatagttc ggccctggtg tgtcacgcca cagggcatta tttacacggt ccttaaacgc
     7561 atccgtctga accagccaca gcgcgacatc gccactggct ctggcacggc gggcacttct
     7621 gccggtatta tccgggaagg tacggttaat cagcctgtca cggcgaagac catccccctt
     7681 gaacagaaac accctgttgc ccagtccgtc actccggcaa cgacgccaga aacgataggc
     7741 gttatctgtc accccggctt cccctcccgt atccaccgcc atggccatca gacgcatgcg
     7801 cacatccgga tcagaagcca gcggccatgt tttatggaac acatccgtca gcaacaaatc
     7861 ccagtcctcc ggatatgccg ccggatcaac cggcagactt tcaccgttgg gactgcatcg
     7921 cagtgaatgc cggatgttgt agcgatcaac aatccagcgt tccccctgct ctccgtatcc
     7981 ggtgatctgc acaacaaaac ggcgattttt accgccctgt acgtcaaccg ttgcctcaat
     8041 aaaacgcaca ccatccggca cagatcgccg gggaaacggc tcggcacgct gttcaagcag
     8101 ttcactttta cgctgttccg tggctgaacg gggcagatag ggtcgtccga tatcggtgtt
     8161 caccaccgct ttcagggtct cttcactgcc ggttcgctca tactcttctt ctgccgccag
     8221 cagcttaaaa atcagttgtt cccaggtctg aaacgccgca gccggcccct ccatccagaa
     8281 acttgctatt ctggaatttc ggggggttcc ggtgatactg ccatccgccg ctgcccgttc
     8341 gccttcccgc agccagatcc cctggttatt cagttcacgc ttctgttccg gagcaatcag
     8401 gccgcggcag tgcggacaca tcaggcgggc cgcctgcccg gcagccacaa aatcagggtt
     8461 attccggtaa ccggtcatgt tatccatcac cggctgaaaa tattccccgc agtgcggaca
     8521 cggccagtac caccggcggc ggtctccccg gttatacagt gacaggatcc ccgttgttgg
     8581 cggtgcctca tgtgcgccac cacaacgcca tttggtatcg gtgatatccc gcccgggcga
     8641 actttcgacc agggtcatcc ccgaggacat aaaggtggtg gtacgctttg aggccagggt
     8701 gaaggcatcc ccttccccgt ccacgttttc agggaaacgg tcataatccg tcagcgccac
     8761 acgacggtaa tccgaggagg aaaatacggt gatcgacggc cagccaattt tcaggaatga
     8821 gccgtcaaga aacattttat cgtggacgtt gttgtcatta cgggaagggc tgaggcgttt
     8881 gcagacctcc gggctgtggc gaaacgtcct ggaaagacgc gttctggaat gctcacgcgc
     8941 cttcgtctcg gtcatctgca ccaccagcat gtcagccgga tcacagatga tgccgtacac
     9001 aatccagcca tcaatcagcc cttcggtttt cccggttcgc gccggtccga caaacaccac
     9061 cgcgtcatat tcacgggctg ataatgtatt aatggggtct atcatatagg gcgtcagcga
     9121 tgactcccac ggaccggaag tattggctcc ccgtggtacc cgcatataac gcctgatggc
     9181 ttccgctact ggtaaccggc caggtgggcg aaacagcgag gccacttcgc gccagatatc
     9241 ggatgcgcgg ctatggctct cgttcacctg attcacatat cggcctcatc acaacagtca
     9301 atgactgcct tttccagtgt gtcgcggatc tcatcaacca caatctgtac ttcattcagt
     9361 tgtgatgcgg tccaccccct gtccctctcc agccggtcag gccaggtttc cagtacctga
     9421 actatcgctt tcaccacgac agaaaacgac cgcctgacat cactgacagg cacgagcaga
     9481 ccggtttcct gctcaaattt cagtcggtca cgttctgact ggtaccatgc tttacgcgca
     9541 tgaggatcca tttcctcgtt atctacaggc agaggagctt tcatcagttc ggcaaggata
     9601 tctgtcagtc ggtacagttt gagattgctc tcatgaccac cggctgggct tatgtttttt
     9661 acccgagccg cgacagtctg tcgatgagca ccggataatg cggccagttg ggaaatattc
     9721 agatgcagat tttttaattc acgatccata
//
LOCUS       DAZGQL010000153         9723 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.153, whole genome shotgun sequence.
ACCESSION   DAZGQL010000153 DAZGQL010000000
VERSION     DAZGQL010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9723
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.153"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(18..1391)
                     /locus_tag="ACHKIR_004712"
                     /pseudo
     CDS             complement(18..1391)
                     /locus_tag="ACHKIR_004712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            complement(1590..2636)
                     /gene="fbpC"
                     /locus_tag="ACHKIR_004713"
     CDS             complement(1590..2636)
                     /gene="fbpC"
                     /locus_tag="ACHKIR_004713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric ABC transporter ATP-binding protein"
                     /protein_id="HFO9992351.1"
                     /translation="MSQKNFVELRNVTKRFGSNRVIDNINLTIPQGQMVTLLGPSGCG
                     KTTILRLVAGLEKPSEGQIFIDGEDVTHRSIQQRDICMVFQSYALFPHMSLGENVGYG
                     LKMLGVSRSEVKQRVKDALTMVDLEGFEDRYVDQISGGQQQRVALARALILKPKVLLF
                     DEPLSNLDANLRRSMRDKIRELQKQFNITSLYVTHDQSEAFAVSDTVLVMNKGHIMQI
                     GSPQDLYRQPASRFMASFMGDANLFPANFSEEYVDIYGYRLPRAAHFPAQGSGTAGVR
                     PEAITLNNHGEESQRCVIRHVAYMGPQYEVTVEWHGQEILLQVNATRLQPDIGEHYYL
                     EIHPYGMFVLADAA"
     gene            complement(2648..4726)
                     /locus_tag="ACHKIR_004714"
     CDS             complement(2648..4726)
                     /locus_tag="ACHKIR_004714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO9992352.1"
                     /translation="MSQTLALHPVKKRDAIFLWVLLGWLAFILLPSWSLDYGLLESTS
                     DEILEAYGWSHFNISWLWYLLPSLLLVRPFREATREQRSRHYFDASWALLCMAFIVIS
                     ATVAGRGLGYAAIVLFVELGAIMTLALTRLEWLGGDRFVIGSLIAIVALIGIFIVWPS
                     IAIFIPMFTNDAGEFAPLAFMNVLSQAHIVQVIVNSIVLSIAVGIGCTFFGLVLAIYT
                     TRIAKRSAIIGRIFSILPIVTPPFVVGLGVTLMMGRSGYITEFMVEWFGLTNTNWLYG
                     FTGIWLAQVLAFTPMAFMILDGAIKTIHPSLEEASYTLRASRWQTFNGVFVPLLKPAL
                     ANAFLIVVVQSLADFSNPLVLGGNFDVLATQIYFYITGSQLDYQAASTLGAFLLLFSL
                     LVFCVQYMWIGKRSYVTVSGKSYRGDVQPLPVTLVWSVVAILTVWIAFNALLYGSIFY
                     GSFTVNWGVDYTLTLDNFIKLFGQGMSDGAWPSLLDTLLYAGIAAPITAIFGLLIAWV
                     VVRQQFKGKKTIEFTTMLCFAVPGTVAGVSYILAFNSAPLYLAGTAAIVIISMVMRNV
                     PVGIRAGIAGLGQIDKSLDEASLSLRAGSLRTITHILLPLLRPAILSALIYSFVRAIT
                     TVSAIVFLVTPDTRVATAYILNRVEDGEYGVAIAYGSILIVVMLAIIFLFDWLIGEAR
                     ISRSKAKNQA"
     gene            complement(4795..5826)
                     /locus_tag="ACHKIR_004715"
     CDS             complement(4795..5826)
                     /locus_tag="ACHKIR_004715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO9992353.1"
                     /translation="MKMTFTSTLIASAVALATLTGAAQAKGRLVVYCSATNEMCEAET
                     KAFGEKYDVKTSFIRNGSGSTLAKVDAEKKNPQADVWYGGTLDPQSQAGEMGLLQAYK
                     SPNLEQIMEKFRDPAKVKGNLSSAVYVGILGFGVNTDRLKEKNLPVPKCWKDLTKPEY
                     KGEIQIADPQSSGTAYTALATFAQLWGEDQAFDYLKQLNANVSQYTKSGIAPARNAAR
                     GETAIGIGFLHDYSLEKEQGAPLELISPCEGTGYEIGGVSILKGARNLDNAKLFVDWV
                     LSKEAQELAWKKGKSYQILTNTTAETSPNSLKLDDLKLINYDMDKYGSTDVRKALINK
                     WVSEVKMGK"
     gene            complement(5823..7139)
                     /gene="uhpC"
                     /locus_tag="ACHKIR_004716"
     CDS             complement(5823..7139)
                     /gene="uhpC"
                     /locus_tag="ACHKIR_004716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HFO9992354.1"
                     /translation="METTMHARSAREINQCYRTLRPRLLMCMIIGYAAFYLTRKSVNY
                     VLPALQTDLGLDKGDIGLLGSLFYLSYGLSKFTAGLWHDSHGQRGFMGVGLFATGLLN
                     VVFAFGESLTLLLVVWTLNGFFQGWGWPPCARLLTHWYSRNERGFWWGCWNMSINIGG
                     AIIPLISAFAAHWWGWQAAMLTPGIISMALGIWLTLQLKGTPQEEGLPTVGHWRHDPL
                     ELRQEQQSPPMGLWQMLRTTMLQNPLIWLLGVSYVLVYVIRIALNDWGNIWLTESHGV
                     NLLNANATVMLFEVGGLLGALFAGWGSDLLFSGQRAPMILLFTLGLMVSVAALWLAPV
                     HHYALLAVCFFTVGFFVFGPQMLIGLAAVECGHKAAAGSITGFLGLFAYLGAALAGWP
                     LSLVIERYGWSGMFSLLSVAAVLMGLLLMPLLMAGITTTHARRIKQ"
     gene            complement(7212..8753)
                     /locus_tag="ACHKIR_004717"
     CDS             complement(7212..8753)
                     /locus_tag="ACHKIR_004717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HFO9992355.1"
                     /translation="MSRNLRHWVISLFIVLAWGTGWLMLWTLSFYLTNNGQQAVLFLP
                     QGVYLALLILLSRRYWPALVLPPLLMMFWLHSEQLLNGYLMLATPVISLFPAILAQNF
                     WHRFPLYWQRLTLLLATVTAASLLNTALLSPFMSGPIMLPGLTSFTGGVLLTPFVYLI
                     FEFLRQQHRYQLLGLDTHNPPLRTSLIIWCSLFFIIGIGTQMVLSPEIERLLLIVVFL
                     PNVVMAWKFGWQGGVLSGLLGSMMITIARQIGVGFSNLVELEIFLATQALLGIGLGIA
                     ISRQQHLALNLHHYRQRLEAELAARRALAEKLIHTEEDTRKNLARELHDEIGQNITAI
                     QIQSQLVKRARDPAQIQSAASQINELARRIHLSTRQLLRQLRPPALDELTFREALLHL
                     INEFAFSERGIHCQFAYQLNSTPENETVRFTLYRLLQELLNNVCKHAEASEVTIILRQ
                     QGEVLHLEVSDNGVGIASGKMAGFGIQGMRERVSALGGDLTLEKQHGTRVIVNLPTFL
                     QQIAG"
     gene            complement(8753..9382)
                     /locus_tag="ACHKIR_004718"
     CDS             complement(8753..9382)
                     /locus_tag="ACHKIR_004718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFO9992356.1"
                     /translation="MIRVVLVDDHVVVRSGFAQLLSLEDDLEVIGQYSSAAQAWPALI
                     RDDVNVAVIDIAMPDENGLSLLKRLRAQKPQFRAIILSIYDAPTFVQSALDAGASGYL
                     TKRCGPEELVQAVRSVGLGGHYLCADAIRALRGGGQPAQALEILTPREREVFDLLVKG
                     DSVKEIAFKLELSHKTVHVHRANVLGKLNCHSTIELVHFALDHHLLAGH"
BASE COUNT         2398 a         2662 c         2543 g         2120 t
ORIGIN      
        1 agctggctgt ctcttgatca gatatcctga tcaagagact tcatcaccag gtaaccctca
       61 accatatcct gaagtctgaa ccagtcatcc cacataacta cccaaccggg gcggcctgtg
      121 cgtttgctgt catgtcatcg ccccagttta gccagtttca gactggctca tttcaggatc
      181 ggtgcctgcg ccggaagcgg ctctccttcc agcttaaccc acagcagttt ccactctgtc
      241 ggcgtcagta ttttctcaca actgtcattt tgtgtttctt cactgacacc tccctgccgc
      301 aggcccggca cccgtaccgc gataaacgcc ttgataacca ccatgcgctc aaggttatcc
      361 cgggtctgaa ttcgcagcga ttccacacat gtaccaccac ttttccacgc cttgtggtat
      421 tcctctatca gccagcgtcg ctcgtaatgg ctgacgatac gtcgcgcatc ggcggcactc
      481 gccacttttt ctgacgtcag cagatgccag caggcactgt cctctgcctg ctcccggcaa
      541 cagacatacg tgagcgagag cgcctggccg ctgttgtcgg gattttttat gctgacttcg
      601 ctgtaactga tgaacatccg ggcctggcgg gctgcccgcc cgcctttttg catcacattc
      661 agcgtgtggc ttcccgcggt tgccaggact tccggcagtt cgaagagctt gccgggtgct
      721 tcttccagcc ggctattctg tgcagcacgc accacgaagc gctgtccgtg gctgacttta
      781 taatgcaggt aatgccagat atccgcttcc cggtcacaga cagtgattac ccgtttctgt
      841 atctccccca gccgttcggc catgcgctcc gaagcctgct gccagcggta actttctttt
      901 tcttcacagg gacgttcttt tcgctggtgc ttaacaccgt aggtgtccgt ggcccgactc
      961 cagctctgct gttcgataag agcgactggc agggcgctgt cgggggcgta tatcaggaca
     1021 gagtgagcca gcagcccgcg cgtcttcggg ttagtggtgg tattccccag gtcatcagat
     1081 gccgtactgt ggctgaagtt aatggtggag gtgtcttcca gtgcggggag cagcggatgt
     1141 gcctcacatg cccttgcagt ggcggtaaat ccggctccgg aaatggcttc cggggacact
     1201 gacgggttac gcatcaggcg gtacgcacct tcaacctgag cagtggagtg ggattatttc
     1261 acaatagaaa gacctgcatg ctgagcgaga gaagaggcca gtgacacaag gcgtcgtgta
     1321 cgacgcggat caccgagacg ggcatgtcca gactgctcgt tagcccatga ataacaatca
     1381 gaaagtacca taacagaccc gaagaaaatg aaatataaga gaagatcaac gggtgaagaa
     1441 aaagttcaaa aaatcgctgc cggggaggaa ggaaactacc ggatggaaag agtcccccct
     1501 aaagcagact gacagacatc acaaatcccc aggtggtgat ttgtgtataa gagacagctg
     1561 agctggaggg gaccgcttat gcgctatttt tatgccgcat ccgctaaaac aaacatcccg
     1621 taagggtgga tttcgaggta atagtgctca ccaatatcgg gctgtaaacg ggtggcgttg
     1681 acttgcaata aaatctcctg accatgccat tctacggtca cttcgtactg cggccccatg
     1741 taagcgacat ggcgaataac acaacgctga ctctcttcgc cgtgattgtt taaggtgatg
     1801 gcttccgggc gaacgccggc ggtgccgcta ccttgcgccg ggaaatgcgc tgcgcgcggc
     1861 aggcgataac cgtagatatc gacatactct tcgctaaagt tcgccgggaa caggttggcg
     1921 tcgcccataa aacttgccat aaatcgggag gctggctgac gatagagatc ctgcggtgag
     1981 ccaatctgca tgatgtgacc tttattcatc accagcacag tgtcggacac cgcgaaagct
     2041 tcactttgat cgtgagtcac gtagagtgac gtgatattaa actgcttttg cagctcgcgg
     2101 atcttatcgc gcatgctgcg gcgcaggttg gcatcgaggt tacttaacgg ctcatcaaac
     2161 agcagcacct ttggcttgag gatcaacgca cgggccagag ccacacgctg ttgctgacca
     2221 ccggaaatct ggtcgacata gcggtcttcg aatccttcca gatccaccat tgtcagcgca
     2281 tctttcaccc gctgcttcac ttcactacgc gacacgccaa gcattttcag cccatagcca
     2341 acgttttcgc ccagcgacat atgcgggaac agagcgtatg actgaaacac catgcagata
     2401 tcgcgttgct gaatggaacg atgcgtgaca tcttcgccat ctataaaaat ttgcccttcg
     2461 ctcggttttt ccagcccggc aaccagacgc agaatggtgg ttttgccgca tccggaagga
     2521 ccgagcagcg tcaccatttg cccttgtggg atggtgagat tgatattgtc gataacccta
     2581 ttactgccga atcgtttagt gacgttgcgc agttccacaa aatttttctg actcatagtg
     2641 cgctccatta cgcctggttt ttggctttag aacgggaaat acgtgcttca ccgatcagcc
     2701 agtcaaagag gaaaataatc gccagcatca ccacgatcag aatcgaaccg taggcgatgg
     2761 ctacgccata ttcgccgtct tctacgcggt taaggatgta ggcggttgcc acgcgtgtgt
     2821 ccggtgtgac gagaaagacg atggcgctga ctgtagtgat ggcccgcaca aagctgtaaa
     2881 tcagggccga gagaatggcc gggcgtaaca gcggcagtaa aatgtgcgta atggttcgca
     2941 gggaacccgc acgcaggcta agtgaggctt catccagcga tttgtcgatc tgccccagac
     3001 ctgcaatccc ggcgcgaata cccaccggaa cgttacgcat caccatcgag ataataacaa
     3061 ttgctgccgt tccggcgaga tagagtgggg cgctattaaa ggcaagaata tacgagacac
     3121 ccgccactgt acccggtacg gcaaagcaca gcatggtggt gaactcgatg gtctttttgc
     3181 ctttgaattg ttgacgcacc accacccagg cgatgagcag gccaaagata gcggtaatgg
     3241 gggctgcaat cccggcgtag agcagggtat ccagcagtga aggccacgcg ccgtcgctca
     3301 tcccctgacc aaataacttg ataaagttat ccagcgtcag ggtgtagtca acgccccagt
     3361 tcaccgtgaa gctgccgtag aaaatgctgc cgtagagcag ggcgttaaag gcaatccata
     3421 ccgtaagtat ggcgaccacg ctccacacca gggtgaccgg tagcggctgg acgtcgccac
     3481 ggtaggattt gccggaaacc gtgacgtaag agcgtttacc aatccacata tactggacgc
     3541 agaacaccag cagcgagaac agcagcagga aagcaccaag cgtgctggcg gcctgataat
     3601 ccagctgcga gccggtaatg tagaagtaaa tttgcgtcgc cagtacgtcg aagttgccgc
     3661 caagcaccag cgggttactg aaatcagcca gtgactgcac cacgacgatt aaaaaggcat
     3721 tggcgagcgc gggctttagc aatggcacaa acacgccgtt aaaggtttgc cagcggctgg
     3781 cgcgcagggt ataggaggct tcttccagcg agggatgaat ggttttaatg gccccgtcga
     3841 ggatcataaa agccattggc gtgaatgcca gaacctgcgc cagccagatc ccggtaaaac
     3901 catacagcca gttggtgttg gtcagcccaa accactccac cataaactcc gttatgtagc
     3961 cggagcgccc catcatgagc gtcacgccta agccgaccac aaaaggtggc gtaacgatgg
     4021 gcagaatgga aaaaatgcgg ccaataatgg cgctgcgttt ggcgatgcgc gtggtataaa
     4081 tcgccagcac caggccgaag aaggtacagc caatacccac cgctatcgac aagacgatag
     4141 agttgacgat cacctgcaca atatgtgcct gcgagagcac gttcataaac gccagcgggg
     4201 cgaactctcc cgcgtcgttg gtgaacatcg ggataaaaat ggcgatgctc ggccagacga
     4261 taaaaatgcc aatcagcgcc acaatcgcaa tcagtgagcc gatcacgaag cggtcgccgc
     4321 ccagccattc gaggcgggtt aaggccagcg tcataatcgc gcctaattca acaaacagca
     4381 cgatggcggc gtagcctaaa ccgcgtccag ccacggtggc gctgatgacg ataaacgcca
     4441 tacagagcaa tgcccagctg gcatcaaaat aatgacgact acgctgttcg cgtgtggcct
     4501 cacgaaacgg acgaaccagc aacagactcg gtaataaata ccacagccag ctgatattaa
     4561 aatgcgacca gccataagcc tcgaggatct catcactcgt ggactccagc aggccataat
     4621 caaggctcca gctcggcaac aggataaatg ccagccagcc cagcaaaacc caaaggaaaa
     4681 tcgcatcccg ctttttcacg ggatggagtg caagtgtctg tgacatagca gttccggtat
     4741 ggttggccgg ggcaggcgtt tgtgctgcac ccggcaaatg cgcctaatgc gggtttattt
     4801 acccatcttc acttcgctga cccatttatt aatcaatgcc ttacgaacat ccgtggaacc
     4861 atatttatcc atgtcgtagt tgattaattt caggtcgtcg agcttcagcg aatttggcga
     4921 cgtttcggcg gtggtattgg tcaggatctg ataagacttc cctttcttcc acgccagttc
     4981 ctgagcttct tttgataata cccagtccac aaacagtttg gcgttgtcga ggttgcgcgc
     5041 gcctttcaga atgctgacgc cgccaatttc gtagcccgta ccttcacacg gggaaatcaa
     5101 ctccagcggc gcgccctgtt ctttttccag cgagtagtca tgcaggaagc caataccaat
     5161 cgccgtttcg ccacgggcgg cgttacgtgc cggggctatg cctgatttgg tgtactggga
     5221 gacgttggcg ttcagttgtt ttagataatc aaaggcctga tcttcccccc acagctgcgc
     5281 aaaagtagcc agggcggtgt aggcagtacc ggaactttgc ggatcggcaa tttgaatttc
     5341 gcctttatat tccggtttgg tgagatcttt ccagcacttc ggcaccggca ggtttttctc
     5401 tttcagacga tcggtgttta cgccaaaacc gaggatcccc acatatacgg cagaggacag
     5461 attccctttc acttttgccg gatcgcggaa tttctccatg atctgctcaa ggttagggga
     5521 tttatacgcc tgtagcaggc ccatctcgcc agcctgcgat tgcgggtcta aagtgccgcc
     5581 gtaccagacg tcagcctggg ggtttttctt ttcagcatcg acttttgcca gggtgcttcc
     5641 ggaaccgttg cggataaacg aggtttttac atcatatttc tcgccaaacg ctttggtttc
     5701 ggcctcacac atttcattgg tggcgctgca atagaccacc agccggcctt tcgcctgcgc
     5761 ggcacccgtt aaagtcgcca gcgcaacggc agaagcaatc agagtagagg taaaggtcat
     5821 tttcattgtt ttatccttcg agcgtgagtg gtggtgatgc ccgccatcag taacggcatc
     5881 agcagtaaac ccataagtac tgcggcgacc gagagtaaac tgaacattcc cgaccagccg
     5941 tagcgttcga tgaccagcga cagtggccag cccgcaagcg ccgcgccaag gtaagcgaac
     6001 aggccgagaa atccggtgat tgaacccgcc gcagctttgt gcccacactc caccgccgca
     6061 agtccaatca gcatttgtgg accaaagacg aaaaagccca cggtaaaaaa gcacactgcc
     6121 agcagagcgt agtgatgaac cggtgccagc cacagtgcgg cgactgacac catcaacccc
     6181 agcgtgaaca gcaaaatcat tggcgctcgt tgcccgctaa acagcaaatc cgatccccat
     6241 ccggcaaata acgcgccgag taagccgccc acttcaaaca gcattaccgt ggcgttggcg
     6301 ttgagcaggt tgacgccgtg gctttccgtc agccagatat tgccccagtc gttaagcgca
     6361 atccggatca cataaaccag tacataagag acgcccagta gccagatcag cggattttgc
     6421 agcatggtgg tgcgcaacat ttgccacagc cccatcggcg gactttgttg ttcctgacgt
     6481 aactccagcg gatcgtgacg ccagtgacca actgtcggta atccctcttc ctggggcgtg
     6541 cctttgagtt gtagcgttaa ccagatccct aatgccatgc tgattatccc cggtgtcagc
     6601 atcgcggcct gccagcccca ccagtgggcg gcaaaggcgc taatcagcgg aataatcgct
     6661 ccgccaatgt tgatcgacat attccagcag ccccaccaga aaccgcgctc attgcgtgag
     6721 taccagtggg tcagcaagcg ggcacacggt ggccatcccc agccctgaaa aaaaccgttc
     6781 agtgtccaga ccacaagcag aagcgtaagt gattcgccaa aggcaaacac cacgttcaat
     6841 aacccggtgg cgaacaagcc gacgcccata aaaccgcgtt gtccgtggct gtcatgccac
     6901 agtccggcgg taaatttcga caatccgtag ctgagataaa acagcgaacc cagcaggcca
     6961 atgtcgcctt tatccagtcc gaggtcggtt tgcagcgccg gaaggacgta attcacgctt
     7021 tttcgcgtca gataaaatgc ggcgtagcca ataatcatgc acatcagcaa tcgcggacgt
     7081 aatgtccggt agcattgatt aatttctctc gctgaacgcg cgtgcatggt tgtctccatg
     7141 ttgctgacta tatgaaaatg aaagtgaaag gggatgagag aaaatccggg atacctaaga
     7201 ctttttcctg gttatccggc gatttgttgc aaaaatgtgg gcaagttaac aattacccgc
     7261 gtgccgtgct gtttttcgag cgttaagtcg ccgcccaggg cgctgacgcg ctcacgcatc
     7321 ccctgaatgc caaatccggc cattttcccg ctggcgatcc ccacaccgtt gtcgctgacc
     7381 tccagatgca atacctctcc ctgctggcgc aggataatcg tcacttcgct ggcttcggcg
     7441 tgtttacaga cgttattgag caactcctgc aataaccgat acaaggtaaa gcgcaccgtt
     7501 tcgttttctg gtgtggagtt cagttgatag gcaaactggc aatgaatgcc gcgttcacta
     7561 aaggcaaatt cattaatcag atgcagcaac gcttcgcgga atgtcagttc atcaagcgcg
     7621 ggaggccgca actggcgcag caactggcgg gtggagagat gaatgcggcg ggcgagttca
     7681 ttgatttggc tggctgcgga ttgaatttgc gccggatcgc gcgcccgctt caccagttgc
     7741 gactggatct gaatggcggt gatgttctga ccgatttcgt cgtgcagctc tcgcgccagg
     7801 tttttgcgcg tgtcctcctc ggtgtggatc agcttttccg ccaatgcccg ccgcgctgcc
     7861 agctcggcct ccagccgttg tcggtaatgg tgcaggttta gcgccagatg ttgctggcga
     7921 ctgatggcaa tgcccaggcc aattcccagc aatgcctggg tcgcgaggaa aatctccagc
     7981 tccaccaggt tactaaagcc cacgccaatc tggcgggcga tggtgatcat catgctgccc
     8041 aaaagcccgg agagtacgcc accttgccag ccaaatttcc acgccatcac cacattgggt
     8101 agaaaaacta cgatcaacag caggcgttca atttcaggtg acagcaccat ctgagtgccg
     8161 ataccaatga taaaaaacag gctacaccag atgattaaag aggtacgcaa ggggggatta
     8221 tgcgtgtcaa gccccaggag ctggtagcga tgctgctggc gcagaaactc aaagatgaga
     8281 tagacaaacg gcgtcagtaa tacgccgccg gtaaatgagg tcaaccccgg cagcattatt
     8341 gggcctgaca taaaagggga gagcagggcg gtattcaaca atgaagctgc cgtgacagtg
     8401 gctaacagca acgtcagacg ctgccagtaa agtggaaaac ggtgccagaa gttttgcgca
     8461 aggatggcag gaaacaggct aataaccggc gttgccagca tgagataacc gttcaatagc
     8521 tgttcactat ggagccagaa catcatcagc aaaggtggga gcaccagcgc aggccagtag
     8581 cggcgagaga gcaaaataag aagggcgagg taaaccccct gcgggagaaa caacaccgcc
     8641 tgttgaccgt tattggtcag ataaaagctg agcgtccaca gcatcagcca gccagttccc
     8701 caggccagca caataaacag agaaatcacc caatgacgaa ggttgcgcga cattaatgcc
     8761 ccgccagcag atggtggtcg agcgcaaaat gcaccagctc aatggtacta tggcagttca
     8821 gttttcccag cacattggcg cgatgcacat gtaccgtttt atgactgagt tcgagcttaa
     8881 aggcgatctc tttaacgctg tcacctttga ccagtaaatc aaacacttca cgttctcgtg
     8941 gggtgagaat ttccagcgcc tgggcgggtt gcccgccgcc gcgtaaagcc cgaattgcat
     9001 cggcacagag ataatgcccg cccagcccga ccgagcgcac cgcctgtact aactcttccg
     9061 gcccacagcg tttggtcaaa tagccgctgg caccagcatc cagcgcgctt tgcacaaagg
     9121 ttggcgcgtc ataaatgcta agaataatgg cgcgaaattg cggtttttgt gcacgcaggc
     9181 gcttaagcaa actgaggccg ttttcgtccg gcatagcaat atcaatgacg gcaacattga
     9241 cgtcatcacg gattaatgcc ggccatgcct gtgcggcact actgtactga ccaatgacct
     9301 ccagatcgtc ttccagagag agtaattgtg cgaagccaga gcgcaccacc acatggtcat
     9361 ccaccagcac aacccgaatc atacttttct tctcttatta tctcattctt ctatcatgca
     9421 tcacggcgcg tcgctggcaa tcaaccagcg acagatatgg aatggatgta acaaaaaatg
     9481 taatttgtta ttcaatataa ttatgcttta tttcatgagt ggctctatat ctggtgttca
     9541 gattattgtc tgcaggggca gttcagaaga tgtgagcaaa taaaaaagaa aacataaata
     9601 aaaccatccg gctttcgggg ccagatggtt attcattatt tttatgtcac tgattcatca
     9661 ggtttgctca gaaagcgaat caatgtagtc agcataccac tgcatcattt cacggcgacc
     9721 atc
//
LOCUS       DAZGQL010000154         9489 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.154, whole genome shotgun sequence.
ACCESSION   DAZGQL010000154 DAZGQL010000000
VERSION     DAZGQL010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9489
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.154"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            204..455
                     /locus_tag="ACHKIR_004719"
     CDS             204..455
                     /locus_tag="ACHKIR_004719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003980153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFO9992357.1"
                     /translation="MTGWELRIWRKSMLWSREKAAREFGVTQRTWHAWENAEQVDVTV
                     WRTTQALSVRDLLPHMQGMRKADIIRRLENELGETAEDV"
     gene            complement(486..680)
                     /locus_tag="ACHKIR_004720"
     CDS             complement(486..680)
                     /locus_tag="ACHKIR_004720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000150316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992358.1"
                     /translation="MTIAERLIQKGFDEGFKQGFKQGALEVAREIACRLRDIGWPPER
                     IQEATGLSGEELKKLFPDEQ"
     gene            complement(742..1704)
                     /locus_tag="ACHKIR_004721"
     CDS             complement(742..1704)
                     /locus_tag="ACHKIR_004721"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004536 - DNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFO9992359.1"
                     /translation="MSKKKNTTTPTPHDAAFRSFLANPDVARDFLELHLPAEYRQLCD
                     LSTLKLEPASFVEPDLHQYASDILWSVKTTGGKDGYVYTLIEHQSTENLYMPFRMLRY
                     SVAAMQRHLEQYKTLPLVIPVLFYHGERSPYPYSMNWLDCFEDPALAAKIYTKPFPLV
                     DITVVDDNEIMNHRRMAALTLLMKHIRHRDMMELLDKLPQVMVEISDEQVRVLIHYIV
                     NAGDSVSPEFMRALAERLPQHEDKLMTIAERLEQQGIQKGIQQGIQQGIQQGIQQGIQ
                     QGIQKGRMEEKQDIARQLQKTGMPSAQICQITGLSEAELKNITH"
     gene            complement(1701..2090)
                     /locus_tag="ACHKIR_004722"
     CDS             complement(1701..2090)
                     /locus_tag="ACHKIR_004722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein CbiX"
                     /protein_id="HFO9992360.1"
                     /translation="MKSCFDTDKDFQMSSDEVTFNADSVRSKLELLSEELMHRCGLVY
                     ELYQRRLSAAIDNFVATLPTEQHLLVLELAREEFDYLSAEEIAEEIRRDAERGYCCHG
                     FDRNCCPLGCGDLDDDCCDAADLMEEP"
     gene            complement(2280..2546)
                     /locus_tag="ACHKIR_004723"
     CDS             complement(2280..2546)
                     /locus_tag="ACHKIR_004723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992361.1"
                     /translation="MRPSIIFATAEYVKRLREECLRENKPLHRHTRFRRQELAPDEIN
                     PDVLAMGGHIARRCSERKRVRIPAMKVSEWGHLLRALEIERVCH"
     gene            complement(2639..3073)
                     /locus_tag="ACHKIR_004724"
     CDS             complement(2639..3073)
                     /locus_tag="ACHKIR_004724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="post-segregation killing protein PndC"
                     /protein_id="HFO9992362.1"
                     /translation="MTVSSTISVFCRDGVFRTVYCHLHGEPTWNGRILHTHYATGQQA
                     EALVEHGDIRCLGPRCDKPAGHTLQNPVDGVTAYYGRDSGFRMDSEAREYRSFMEAIA
                     TESTEEVRFHYVFIDGYWKVMYRTPEGWKMKALALALRRCPE"
     gene            complement(3803..4303)
                     /locus_tag="ACHKIR_004725"
     CDS             complement(3803..4303)
                     /locus_tag="ACHKIR_004725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFO9992363.1"
                     /translation="MSVVAPAVYVGTWHKYNCGSIAGRWFDLTTFDDERDFFAACRAL
                     HQDEADPELMFQDYEGFPGNMASECHINWAWVEGFRQARDEGCEEAYRLWVDDTGETD
                     FDSFRDAWWGEADSEEAFAVEFVSDTGLLADVPETVALYFDYEAYARDLFLDSFTFID
                     GHVFRR"
     gene            complement(4765..5361)
                     /locus_tag="ACHKIR_004726"
     CDS             complement(4765..5361)
                     /locus_tag="ACHKIR_004726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992364.1"
                     /translation="MMKSDEKYQVPVWMRPLLPLLCNTGGNDPEELLNDTETTASANI
                     VRYVLIVAVRSQIDLLQLLYRKGLLRTEIPGGFSPEEAQELLDNLVRSHISKALSGER
                     MAARDRNADLAWIRQQLVDAAWFVRATLEAHGMGVGNESPSAPPETMPDIQTRELVML
                     IKRLASSLKAVKPDSSVVRAAQDWLCDRKLVDITDILR"
     gene            complement(5358..6077)
                     /locus_tag="ACHKIR_004727"
     CDS             complement(5358..6077)
                     /locus_tag="ACHKIR_004727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HFO9992365.1"
                     /translation="MSARSRALIPLSAEQQAAMQAVAVTEQRRRQGRTLSAWPYASAF
                     FRCLNGSRRISLTDLRFFAPALTKEEFHGNRLLWLAAVDKLIESFGELCVLPLPSDAG
                     HRLFPSVPFREGERRRQKTTLTEQKYSRQREREAERRELEYQTCFAQAQIDLAFHTPA
                     TVGSWLSRWSGVVEEHDLETIFWGWCGRFPSLSSFDRFFWQEEPLWRLIFEAGEAGRG
                     APVQVRALEQWMIPNKLENTI"
     gene            complement(6074..6508)
                     /gene="psiB"
                     /locus_tag="ACHKIR_004728"
     CDS             complement(6074..6508)
                     /gene="psiB"
                     /locus_tag="ACHKIR_004728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HFO9992366.1"
                     /translation="MKTELTLNALQSMNAQEYEDIRAAGSDMRRNLTHEVMREVDAPA
                     NWMMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQLWMLVLVNCGGQPFAVV
                     QVQHIFTPVAISHTLALAATLDAQGYSVNDIIHILMAEGGQA"
     gene            complement(6563..8521)
                     /locus_tag="ACHKIR_004729"
     CDS             complement(6563..8521)
                     /locus_tag="ACHKIR_004729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFO9992367.1"
                     /translation="MSVTESKAKTERKSSRKPAKTQETVLSALLAQTEDVSVPLASLI
                     KSPLNVRTVPYSAESVSELAESIKGVGLLQNLVVHALPGDRHGVAAGGRRLAALNMLA
                     ERGIIPADWPVRVKIIPQELATAASMTENGHRRDMHPAEQIAGFRAMAQEGKTPAQIG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTARQVQVFEAACQ
                     SGWGGKPEVQTIRRLVTESEVAVAGNSKFRFVGADAFSPDELRTDLFSDDEGGYVDCV
                     ALDAALLEKLQAVAEHLREAEGWEWCAGRMEPVGSCREDAGTYRSLPEPEAVLTEAEE
                     ERLNELMTRYDALENQCEESDLLEAEMKLMRCMAKVRAWTPEMRAGSGVVVSWRYGNV
                     CVQRGVQLRSEDDAAADADRTEQVQEKASVEEISLPLLTKMSSERTLAVQAALMQQPD
                     KSLALLAWTLCLNVFGSGAYSKPAQISLECKHYSLTSDAPSGKEGAAFMAMMAEKARL
                     AALLPEGWSRDMTTFLSLSQEVLLSLLSFCTACSIHGVQTRECGHTSRSPLDTLESAI
                     GFHMRDWWQPTKANFFGHLKKPQIIAALNDAGLSGAARDAEKMKKGDAAEHAEFHMKD
                     NRWVPGWMCTPRPQMDATEHTTNLADAA"
     gene            complement(8585..8818)
                     /locus_tag="ACHKIR_004730"
     CDS             complement(8585..8818)
                     /locus_tag="ACHKIR_004730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HFO9992368.1"
                     /translation="MSEYFRILQGLPDGPFTRKQAEAVAAQYRNVFIEDDNGEHFRLV
                     VRNNGAMVWRTWNFEDGAGYWMNHVIRDFGILK"
     gene            complement(8876..9403)
                     /locus_tag="ACHKIR_004731"
     CDS             complement(8876..9403)
                     /locus_tag="ACHKIR_004731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO9992369.1"
                     /translation="MSARGINKVILVGRLGNDPEVRYIPNGGAVANLQVATSESWRDK
                     KTGEMREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTMQMLGSAPLQNAQAQPQPQQNGQPQSADATKKGGAKTKGRGRKAAQPEPQPQ
                     PPEGEDYGFSDDIPF"
BASE COUNT         2059 a         2815 c         2524 g         2091 t
ORIGIN      
        1 gaagaaaaac gacggcacaa caatggcatg tcgtggtcac tggtgcacca ctcccgcaca
       61 ggtgaaaaca gagaaaacaa cacgacatat acaatcaaaa aaaagtgaaa tttcttcact
      121 ttttcactct tttttatcgc gctgacttgg tgactatgat gataaaataa agtgaagttt
      181 tttcactttt tcaaaggtgt tttatgaccg gctgggaact gcgaatctgg cgaaaaagca
      241 tgctctggtc acgcgaaaaa gcggccagag aatttggcgt gacacagaga acatggcacg
      301 cctgggaaaa tgcggaacag gtggatgtca ccgtctggag gaccacccag gcactttcag
      361 tccgtgacct gctgcctcac atgcagggga tgcgtaaggc agatatcatc aggcgactgg
      421 aaaatgaact gggggaaacg gcagaggatg tgtgagcgtg attcacagcc tggctgaatg
      481 ccaggctact gctcatcagg aaacagcttt ttcagttctt caccggaaag tccggttgcc
      541 tcctgaatcc gttccggcgg ccagcctata tcccgaagcc ggcaggctat ttcccgcgct
      601 acttcaagag ccccttgttt aaaaccttgt ttaaaaccct catcgaaacc tttctgtata
      661 aggcgttctg caatcgtcat gctgtttgct ctgctcctgc tgacaaaaaa cccggcattt
      721 tgccgggctt tgtggttctc atcagtgagt gatgttcttc agttcagctt cggaaagtcc
      781 ggtgatctga cagatttgtg cagatggcat tcccgttttc tgaagctgac gcgcaatatc
      841 ctgcttctcc tccatgcggc ctttctgtat tccctgctgt attccctgct gtattccctg
      901 ctgtattccc tgctgtattc cctgctgtat tcctttctgt attccttgct gctcaagacg
      961 ttcggcaata gtcatcagtt tatcctcatg ctgcggcaga cgctcagcca gcgcccgcat
     1021 aaattccggt gatacagagt cccctgcgtt aacgatgtaa tgaatcagaa cacgcacctg
     1081 ttcatctgaa atttccacca tgacctgcgg gagtttgtcc agcagctcca tcatgtcacg
     1141 atggcggata tgcttcatca gcagcgtcag tgcggccatc cggcgatggt tcatgatttc
     1201 attgtcatca acgacagtga tatcaaccag cggaaacggc tttgtgtata ttttagccgc
     1261 aagcgccgga tcctcaaaac agtccagcca gttcatgctg tacgggtacg ggctgcgctc
     1321 accatgatag aacagcaccg gaatcaccag tggcaatgtt ttgtactgct ccagatgtct
     1381 ctgcatcgcc gccacactgt aacgtaacat gcggaaaggc atgtacagat tttcggtgct
     1441 ctggtgctcg atgagcgtat aaacatagcc gtctttaccc ccggtggttt tcacactcca
     1501 gaggatatcg ctggcgtact gatgcaggtc cggctcaaca aaggaggcgg gttccagctt
     1561 cagcgtggac aggtcgcaca actgccggta ctccgccgga agatgcagtt ccagaaaatc
     1621 tctggcgacg tcggggttcg ccaggaacga ccggaacgcg gcatcatgcg gcgtgggcgt
     1681 tgtggtgttt ttcttcttac tcacggttcc tccatcaaat cagcagcatc gcagcaatca
     1741 tcgtccaaat ccccacaacc aagaggacag caattgcgat caaagccgtg acaacaatag
     1801 ccacgttctg cgtcacggcg tatttcttct gcaatctctt cagcggaaag gtaatcaaac
     1861 tcctcacggg ccagttccag gaccagcaga tgttgttctg tcggcaatgt agccacaaaa
     1921 ttatcaatag cagcactcaa acgtcgctga tatagctcat aaacaagacc acaacggtgc
     1981 attagttctt cggataatag ttccagtttt gagcgaacgc tgtcagcatt gaaagtgact
     2041 tcatcagatg acatctgaaa atctttgtca gtgtcaaaac aagacttcat ttttatctcc
     2101 tgcccgtccg ggccctgaat gaaagagaac ctgtttccct tctctgtttt ttatggaaca
     2161 ggaggaatcc ggtaccatgc gacatagacg tcatgatgtt tttcatcttg ctcacggttc
     2221 ctcctggtaa agtacctgaa catatcctgc cacctgcaca gacagacggc agggggtaat
     2281 cagtggcaga cccgttcaat ttcaagcgca cggagcaggt gcccccactc gctgactttc
     2341 atggcgggga tacgcacccg cttccgctca ctgcagcgtc tggcgatatg accgcccatc
     2401 gccaggacgt ccgggttaat ctcatccggt gccagttcct ggcgtctgaa gcgggtatgg
     2461 cggtgcaggg gtttgttctc ccgcaggcac tcttcacgca gacgctttac atactcggcg
     2521 gttgcgaaga taattgatgg tctcatggca taatctccat agttagtgaa tgagatgccg
     2581 gacttccgta cggggaagcg ccggcggaga aaggcagggt ttatcgccct gctttttttc
     2641 attccggaca gcgacgcagt gccagcgcga gcgctttcat cttccagcct tccggcgtgc
     2701 ggtacatcac cttccagtag ccgtcgatga acacataatg gaagcgcacc tcttctgtgc
     2761 tttccgtggc aatagcctcc atgaaagaac ggtactcacg cgcctcactg tccatccgga
     2821 aaccactgtc acgtccgtaa taagccgtca caccgtccac cgggttctga agcgtatgcc
     2881 cggcgggttt gtcgcaacgg ggaccgaggc aacggatatc accgtgttca accagggctt
     2941 cggcctgctg accggtggca tagtgggtat gcaggatgcg accgttccag gtcggctcac
     3001 cgtgcaggtg gcagtaaacg gtacggaaca ccccgtcgcg gcaaaaaacg gaaatcgtgc
     3061 tgctgactgt catgaaaacg tctccttctg ctgaatcagg tcgccgggcc gaccggttgc
     3121 tcccccggag gggcgaaagc cccttctgcc gtccacttct tcggggtttc tccccgcagt
     3181 ggacctttcc gatggggcag cgacagccac ggcacggggc agttgtccgg gggcagacgc
     3241 aaaagttttt gacgcttgcg gcaaaaagtt ttgtggctgg tacatctccc cctggacggc
     3301 tgacacgtgt tgtggccgct tcagcccccg gaaagtccac agcgggaaac aggccgcagg
     3361 ggtggcggcg gaagggcaga caactgcgcc atcaggcgca gtttccttac acaccgtcag
     3421 gtgtgcaggg agcgcagatc acggcgcgac agaggcggcg cgaccggaac ggtcgctgca
     3481 agccaggttt ttcagacatc ggaccgtagc ggcagcccga aaccgcgcca gcgggttcgg
     3541 cggagactgg cgggtgcgcg agcagctgcc tgtcggagtc gggcctgacg accggatgcc
     3601 gccaggcagc cgggagccga aggtgtcccc gcctccggaa atcagaacaa cagaaacgac
     3661 ataacagaaa aatgcctcac tccatgacgg gcaggaaggg cagcgccgca ggcgcggccc
     3721 tcctgcattt caggcaggcg taagcctgcc agggttttcc gggggcctgg caggccatac
     3781 ccccgccgga gcggggagta aatcaccgac ggaacacatg accgtcaata aaggtgaagg
     3841 agtccaggaa taaatcccgc gcatacgcct catagtcaaa atagagcgcc accgtctccg
     3901 gcacgtcagc cagcaggccg gtatcactga cgaactcaac cgcaaaagcc tcctcactgt
     3961 cagcctcgcc ccaccaggca tcgcggaagc tgtcaaaatc cgtctcaccg gtatcatcca
     4021 cccagagacg ataagcctct tcgcagcctt catcccgtgc ctggcggaag ccttcaaccc
     4081 aggcccagtt gatatgacat tcagaggcca tattccccgg gaatccctca taatcctgaa
     4141 acatcagttc aggatcggct tcatcctggt gaagagcacg gcaggcggcg aaaaagtcgc
     4201 gctcatcatc aaacgtggtc aggtcaaacc agcgtccggc gatgcttcca cagttgtatt
     4261 tgtgccaggt tccaacgtat acagcaggtg caacaacaga cattcccgaa tcctccatag
     4321 tcacatgttc tgcggcaccg ggcggtgccg tgcctccata aaagccgggc gataaggcgg
     4381 ggatgcacag ggcgcaaggg aagccgcagc ctgacgcaca cggctggcgg gagttggagg
     4441 gtgagaccgc gaaatctgag cgggtgaacg accggaaaat cacggcagac gggaaggagg
     4501 gaccgggtga ccgccgcgtg cattttaccc ttgcatcccc gccagcccgg ctaagatgga
     4561 ggcacggcac agcccgcaca cgacgctgag gccacataac tgaaaaccca caaggccatg
     4621 ccccgcatgg ccgggtaaaa gcggagccgc cggcgacttc cttattccgg cgagtgcaga
     4681 ggacacgccg catgacctcc agaccacccc tgatatgaaa cgatcatgat cggcgtgtcc
     4741 ctgcgaaaaa tatacatgcc tggttcaccg gagaatatct gtgatatcca caagttttct
     4801 gtcgcacagc cagtcctgcg ccgcacgcac tacgctactg tccggtttca ccgctttcag
     4861 agaagatgcc agtcgtttga tcagcatcac cagttcccgt gtctgtatgt ccggcattgt
     4921 ctccggcgga gcagagggac tctcatttcc gacgcccatc ccatgcgctt ccagtgtggc
     4981 acggacaaac caggcagcat cgaccagttg ctggcgaatc caggccagat cggcatttct
     5041 gtcacgggct gccattcgct cacccgacag cgccttgctg atatggctgc gcaccagatt
     5101 atccaggagt tcctgcgctt cttccggtga aaagccacct ggtatctctg tgcgcaacag
     5161 tcctttcctg tacagtagct gcagcagatc aatctgcgac cgcactgcaa ctatcagtac
     5221 ataacggaca atattcgcac tggcggtggt ttctgtatca ttcagcagtt cctccggatc
     5281 attaccaccg gtattgcaga gcaacggcaa cagaggtcgc atccacacgg gaacctgata
     5341 tttttcgtct gatttcatca tattgtgttc tccagtttgt tcgggatcat ccactgctca
     5401 agtgcacgta cctgtaccgg tgcaccacga ccggcctcac cggcttcaaa aatcagccgc
     5461 cagagtggtt cctcctgcca gaaaaaccgg tcaaatgatg acagtgacgg aaaacgcccg
     5521 caccaccccc agaaaatcgt ttccagatca tgctcctcaa caacaccaga ccagcgggac
     5581 aaccagcttc cgaccgtggc gggagtatga aacgcaaggt caatctgcgc ctgagcaaaa
     5641 catgtctggt attccagttc ccgtcgttct gcctcacgtt cccgctggcg gctgtatttc
     5701 tgctctgtca gcgtggtttt ctgacgacgc cgctcacctt cacgaaaagg aacggacggg
     5761 aacagacggt gccccgcatc ggatggcagg ggaagaacac agagttcacc aaaactttca
     5821 atcagtttat ccacggcagc cagccacagg agacggttgc catgaaattc ctccttcgtc
     5881 agcgcaggag caaaaaagcg gagatcggtc agcgaaatcc ggcgactgcc attcaggcag
     5941 cgaaagaaag cgctggcata aggccatgct gaaagtgtgc gtccctgacg acgacgttgt
     6001 tcggtgacag ccacagcctg catggcggcc tgttgttctg cgctgagggg gatcagtgcc
     6061 cgtgagcgtg cgctcatgcc tgacctcctt ctgccatcag gatatggatg atgtcgttaa
     6121 cactgtaccc ctgcgcatca agtgtcgcgg caagcgccag cgtgtgactg atagcgacag
     6181 gcgtgaagat atgttgcacc tgaacgacgg cgaaaggctg tccaccacaa ttcaccagaa
     6241 ccagcatcca gagctgagag acgtctcccg gcgaacataa tgccaggtgg aaacgttcgt
     6301 gggctggcgt gaaacgaacc tggacgggga aaaagccccc gaactcactg ccatactcgc
     6361 cattcatcat ccagttagcc ggtgcgtcca cttcacgcat cacctcgtga gtgagattac
     6421 ggcgcatatc gcttccggca gcccggatat cttcatattc ctgtgcgttc atggactgca
     6481 gggcattcag ggtcagttct gttttcatca cggtatctcc cttgctgccg ccccgtctcc
     6541 ggcggggcgg tgtggttgtt catcaggcgg catcagccag gttagtggtg tgttcagtgg
     6601 catccatctg tggacgtggt gtacacatcc agccaggcac ccagcggttg tctttcatat
     6661 ggaactctgc atgttcagcc gcatcgcctt tcttcatttt ctccgcgtcc cgcgcagcac
     6721 cggacagtcc ggcatcattc agggctgcga taatctgcgg ctttttcagg tgtccgaaga
     6781 agtttgcttt tgtcggctgc caccagtcgc gcatgtgaaa accgatggcg ctctccagcg
     6841 tgtccagcgg gctgcgtgac gtgtgaccac actcgcgggt ctgaacgccg tggatgctgc
     6901 acgcggtgca gaaactgagc agggataaca gcacctcctg actgagtgaa aggaacgtcg
     6961 tcatgtcccg tgaccatccc tccggtaaca gggccgcaag acgggctttt tctgccatca
     7021 tcgccatgaa cgcggcacct tccttccctg acggcgcatc gctggtcagc gaataatgtt
     7081 tacattccag gctgatttgt gctggtttac tgtacgctcc gctgccaaac acattcaggc
     7141 agagcgtcca tgccagcagt gccagagatt tgtccggttg ctgcatcagt gccgcctgga
     7201 ctgccagcgt gcgttcggaa gacattttcg tcagcagtgg cagactgatt tcctccactg
     7261 acgctttctc ctgcacctgt tccgtgcggt cagcgtcggc agccgcgtca tcctcgctgc
     7321 gcaactgcac accacgctgg acacacacgt tgccataacg ccaggacacc accacaccgc
     7381 ttccggcacg catctccggc gtccacgccc tgaccttcgc catgcagcgc atcagcttca
     7441 tttctgcttc cagcaggtca gattcctcac actggttttc cagcgcgtcg tagcgcgtca
     7501 tcagttcgtt caggcgttct tcttcagctt ccgtcagcac cgcttccggc tccggcaggc
     7561 tgcggtatgt tccggcatcc tcacggcagg aaccgacagg ctccatacgt ccggcgcacc
     7621 attcccagcc ttcggcttcc cgaaggtgtt cagcgacagc ctggagtttt tccagcaggg
     7681 cggcatcgag cgccacgcag tccacatagc caccctcgtc gtcgctgaac aggtcagtgc
     7741 gcagttcatc tggcgagaag gcatcagccc ccacgaagcg gaatttactg ttccccgcca
     7801 ccgccacttc actttcggtc accagacgac gaatggtctg cacttccggt ttaccgcccc
     7861 atcctgactg gcaggcggct tcaaacacct gcacctgacg cgcggtgtcg ttctccagcg
     7921 ccagcgcctg acagtgctct gtggtgatgc ggtcttctgc cagcgcatca aggatgacag
     7981 gtgcaaggtc tgccagtttc agcattcgct gaacgtggcg gggtgaatag cccagcaaat
     8041 caccgatttg tgcaggtgtt ttgccttcct gcgccattgc gcggaatccg gcaatctgtt
     8101 cggcagggtg catatcccga cgatgaccgt tttcagtcat tgatgcggca gtcgccagtt
     8161 cctgcggaat aattttcacg cggacaggcc agtcagccgg aatgatgcca cgctctgcca
     8221 gcatgttgag tgctgccagt cggcgaccac ctgcggcgac accgtggcgg tcacctggca
     8281 gggcatgaac aaccagattc tgcagcaggc cgacgccctt aatggactca gccagttcgc
     8341 tgacggactc cgcagaatac ggcaccgtgc gcacattcag cggtgattta atcagtgaag
     8401 ccagcggaac gctcacatcc tccgtctgcg ccagcagggc agacaggaca gtttcctgcg
     8461 tttttgcagg tttacggctg gatttgcgct ctgtttttgc cttagactct gtaactgaca
     8521 tatatttctc ctttgaccgg ggtggttgta tgtctgccgg acgcctaccc gtccggcacc
     8581 tctcttactt aagaatgccg aaatccctga tgacatggtt catccagtac ccggcaccgt
     8641 cctcaaaatt ccaggtacgc cagaccattg caccgttatt acggacaacc aggcgaaaat
     8701 gttctccgtt gtcatcctcg ataaagacgt tccggtactg tgcggcaacg gcttcggcct
     8761 gtttgcgggt aaaaggaccg tcaggcagtc cctgaagtat tctgaaatat tcgctcatcc
     8821 tcatatctcc ggtgatgccc cgcgcagggc ggggcggtta tcacagtcag gccagtcaga
     8881 acgggatatc gtctgaaaac ccgtaatcct ccccctccgg cggttgcggc tgaggctcgg
     8941 gctgcgcggc cttacgtcca cggcctttcg ttttcgcgcc accttttttc gtcgcgtcag
     9001 cactctgtgg ctgaccattc tgctgaggct gcggttgtgc ctgagcgttc tgcagtggtg
     9061 cactccccag catctgcatg gtgcccgtgg tcttaacaag aatttcagtg acgtaacggg
     9121 tgataccgtt atcttcccag ctacgggttc ggagttgccc ctcgatatag acctgcgcgc
     9181 ccttgcgcag atattcacca gccacttccg cgagcttgcc gaacagcacc acgcggtgcc
     9241 attccgtctg ctcccgcatc tcccccgtct ttttgtcgcg ccagctttct gacgtggcca
     9301 cctgcaggtt tgccactgcg cccccgttgg ggatgtaacg gacctccgga tcattgccca
     9361 gacgcccgac gaggatgacc ttgttgatac cacgtgcact catgagaacc tcctgactga
     9421 aagatgtgag ataaaccgga taccggagac ggggcgttca tgtgcccctt cagaggagcg
     9481 aaagctcct
//
LOCUS       DAZGQL010000155         9417 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.155, whole genome shotgun sequence.
ACCESSION   DAZGQL010000155 DAZGQL010000000
VERSION     DAZGQL010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9417
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.155"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(220..2487)
                     /gene="adiA"
                     /locus_tag="ACHKIR_004732"
     CDS             complement(220..2487)
                     /gene="adiA"
                     /locus_tag="ACHKIR_004732"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HFO9992370.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            complement(2686..3594)
                     /gene="melR"
                     /locus_tag="ACHKIR_004733"
     CDS             complement(2686..3594)
                     /gene="melR"
                     /locus_tag="ACHKIR_004733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HFO9992371.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            3877..5232
                     /gene="melA"
                     /locus_tag="ACHKIR_004734"
     CDS             3877..5232
                     /gene="melA"
                     /locus_tag="ACHKIR_004734"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFO9992372.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALQDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            complement(5229..5879)
                     /gene="yjdF"
                     /locus_tag="ACHKIR_004735"
     CDS             complement(5229..5879)
                     /gene="yjdF"
                     /locus_tag="ACHKIR_004735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HFO9992373.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRRFGLITRAPEAITP"
     gene            complement(6002..7648)
                     /gene="fumB"
                     /locus_tag="ACHKIR_004736"
     CDS             complement(6002..7648)
                     /gene="fumB"
                     /locus_tag="ACHKIR_004736"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFO9992374.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(7726..9066)
                     /gene="dcuB"
                     /locus_tag="ACHKIR_004737"
     CDS             complement(7726..9066)
                     /gene="dcuB"
                     /locus_tag="ACHKIR_004737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HFO9992375.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
BASE COUNT         2234 a         2337 c         2517 g         2329 t
ORIGIN      
        1 taatattaca aatttttata gtaatttctc atgtacatat ttttttacag gctaaaaaaa
       61 ggataaataa caggcaaaaa aaagcgcggg gtgggaccgc gcaaaaagca tgacattttt
      121 tgtttgcagc aatttttatg aaaacgttgc catacttaaa aggcctcaag ccaggcggcg
      181 taatgttatt taacaattac gccttcagcg gaatagtggt tacgctttca cacacataac
      241 gtggtaaata ccgtcaataa tttcagtccc ttcagtttcg tgttcaaatc cagggaaatg
      301 atggtcccag gattgcagcg agcgtaaata acttacttgc ggactgtttt tatcgccgaa
      361 gttttcacca gacagcagca tcgggattcc tggcggatac gggataactg agtttgccgc
      421 gatgcgtcct ggtaggtttt caatggatac cagttcgaca ttgttgtcga caatcgcgtt
      481 gtacgcttct cgcggggtga tttccgccac cggcaggccg gaataggctt cgttcaaccg
      541 tgcgccaggg ttgttttctt tcagccaggc aaacatggtg tcacccagat cgtgaatccc
      601 catgttcgcg taagtgtcag gatattgttc aacaagttcc ggcatcacct gcgccagcgg
      661 ggtgttggcg tcatagtggc gtttgaagga gcaaagggtg ttaaccagag tcccccattt
      721 cccacgggtc acgcccatag agaacaggaa cataatctgg aagtcagtgg tacgggtagg
      781 cacaatgccg tggcgaccaa gccaggctgt gaccagcgcc gccggaacac cggtttcttc
      841 cagttcacca tcttcaccca ttcccggagc aaggatgctg actttaatcg ggtcgagcat
      901 actccagtta tccggaatat ctttgaagcc gtgccagctt tcgcccggat gcatgaccca
      961 gcagtcctga acggtggtca gcagtttggt tggtgcgtca gcaaagtcat aggttttgcc
     1021 ggtttgtggg tcggtgacga cttctttgtt ccacggtttg aagaaccagc taccgtcagc
     1081 ggtgaactct ttatacagcc gcgccatcgc ctgacggaaa tcaaccgctt cgtcgatcac
     1141 ttcctgcgtc agtgacaggc cgctgttgcc gtccatcatc gacaccgcca cgtcgttgga
     1201 tgcgcagatg gcatacagcg gggaggtggt ggcatgcatc atgtaggcct ggttgaagcg
     1261 ggagaagtta atcgccccac gaccttcacg tacatgaata taagaggcct gtgacagcgc
     1321 attcagcagt ttgtgggtgg agtgggtggc gaaaacggta gggccgttgt gatcgccagg
     1381 ttcgccgcgc atggcatagt gatcggcata gattgggttg aaacgtgcat agccgtacca
     1441 ggcttcgtca aagtgcagac gatcggaggt tttttccagc agatcctgcg cttctttagc
     1501 gttataacac acgccgtcat aggtacagtt ggtcaccacg cagtaagacg gtttttgccc
     1561 ggctttgtct ttggtcagcg ggctttcact gattttcttc tgcaaggttt caggttgcat
     1621 ttcctgcgga tagattggcc cgataatgcc gtagcggttg cggcttggca ccatgtagac
     1681 cggtttcgcg cctgtcagca tcaagccttg ttcgatggat ttatggcagt tacggtcaac
     1741 gaccacgaca tcgttatcgg tcatgcaagc ctgcatgatg gtgcggttag agccggaagt
     1801 accgacgact accgaccagg agcgatcggc accaaatacg cgtgcagcat atttttcgct
     1861 ttcgccaaat gcgccagtat ggtcaagcaa agaaccgagg gaagttcgtt cgatgcccat
     1921 gtcggtgcgg aacagatttt caccatagta gtcatggtag aaacgtccgg cgggtgtttt
     1981 ggtaaaacca acgccgccct ggtggcctgg cgctgcccag gaatattcat ggatgtcgct
     2041 atatttcatc agcgcgctga acagtggcgg caacagttgc tggcggtagc gggtcatcgc
     2101 ggcaacggcg cgtccggcga taaagtcggc ggtatcttcc agaatccagg cgaattcatc
     2161 gacaagctcc agcagatccc ggtccattgc ggcgagggct ttttcccgat cgcccaacag
     2221 gaagaccggc acgttttgtt gacgctcatg aagcttaccg atcaattgtc tgacgttttg
     2281 atgttcgtcc ggatgttcca tttgatagct gaacatcagg cagtcaatgg cttcgtttga
     2341 agagagaatg gcaaaaccat catcaaagga ggtggattta atcacggtaa cattttgctg
     2401 gcttaaagca tctgccagac gctcaacggc gttaccgacc caggtgtctt gatggagaaa
     2461 ctcgctttca acaattaata ctttcatcat tgcttacccg gttatgaagg aatcttcgtt
     2521 gcgggcaagt atcttccggc cttttcgggc tgtaaagcgc gtgaaaaaca aacatttttg
     2581 aaatatttgt ttttataaac aatatgttat gtgattattt taaattattt tcactgctta
     2641 ttatcgacgg ctaaactatt tttttggctg atactgatat cgtctttagc cgggaaacgt
     2701 ctggcggcgc tgttggctaa gtttgcggta ttgttgcggt gacatgccga catatttgcc
     2761 gaacgtgctg taaaaacggc tactcgaacg aaagcctgcc gtcagggcaa tatcgagaat
     2821 acttttatcg gtatcgctca gtaacgcgcg aacgtggttg atgcgcatcg cggtaatgta
     2881 ctgtttcatc gtcaattgca tgacccgctg gaatatcccc attgcatagt tggcgttaag
     2941 tttgacgtgc tcagccacat cgttgatggt cagtgcctga tcatagtttt cggcaataaa
     3001 gcccagcatc tggctgacat aaaattgcgc atggcgcgag acgctgtttt tgtgtgtgcg
     3061 cgaggtttta ttgaccagaa tcggttccca gccagagagg ctaaatcgct tgagcatcag
     3121 gccaatttca tcaatggcga gctggcgaat ttgctcgttc ggactgttca attcctgctg
     3181 ccagcggcgc acttcaaacg ggctaagttg ctgtgtcgcc agtgatttga tcaccatgcc
     3241 gtgagtaacg tggttaatca ggtctttatc cagcggccag gagagaaaca gatgcatcgg
     3301 cagattaaaa atcgccatgc tctgacaggt tccggtatct gttagttggt gcggtgtaca
     3361 ggcccagaac agcgtgatat gaccctgatt gatattcact ttttcattgt tgatcaggta
     3421 ttccacatcg ccatcgaaag gcacattcac ttcgacctga ccatgccagt ggctggtggg
     3481 catgatatgc ggtgcgcgaa actcaatctc catccgctgg tattccgaat atagcgacag
     3541 cgggctgcgg gtctgttttt cgtcgctgct gcacataaac gtatctgtat tcatggatgg
     3601 ctctctttcc tggaatatca gaattatggc aggagtgagg gaggatgact gcgagtggga
     3661 gcacggtttt caccctcttc ccacagcggc gaggggagcg tccgagcatc atgaggccga
     3721 aaactctgct tttcaggtaa cttattccca taaactcaga tttactgctg cttcacgcag
     3781 gatctgagtt tatgggaatg ctcaacctgg aagacggagg ttttctgcag attcgcctgc
     3841 catgatgaag ttattcaagc aagccaggag atctgcatga tgtctgcacc caaaattaca
     3901 tttatcggcg ctggttcgac gattttcgtt aaaaatattc ttggtgatgt gttccatcgc
     3961 gaggcgctga aaacggcgca tattgccctg atggacattg atcccacccg cctggaagag
     4021 tcgcatattg tggtgcgcaa gctgatggat tcagcagggg ccagcggcaa aatcacctgc
     4081 cacacccaac agaaagaagc cttacaggat gccgattttg tcgtggtggc atttcagatt
     4141 ggcggttatg aaccttgcac ggtgactgat ttcgaggtct gtaagcggca tggtctggaa
     4201 caaaccattg ccgatacgtt ggggccgggc ggtattatgc gcgcgctacg taccattccg
     4261 catctgtggc aaatttgcga ggacatgacg gaagtctgcc ccgatgccac catgctcaac
     4321 tacgttaacc caatggcgat gaatacctgg gcgatgtatg cccgctatcc gcatatcaaa
     4381 caggtagggc tgtgccattc ggtgcaggga acggcggaag agctggcgcg tgacctcaat
     4441 atcgacccgg ctacgctgcg ttaccgttgc gcaggtatca accatatggc gttttacctg
     4501 gaactggagc gcaaaaccgc cgacggcagt tacgtgaatc tctacccgga actgctggcg
     4561 gcttatgaag cagggcaagc accgaagccg aatattcatg gcaatactcg ctgccagaat
     4621 attgtgcgct acgaaatgtt caaaaagctg ggctacttcg tcacggaatc gtcagaacat
     4681 tttgctgagt acacaccgtg gtttattaag ccaggtcgtg aggatttgat tgagcgttat
     4741 aaagtaccgc tggatgagta cccgaaacgc tgcgtcgagc agctggcgaa ctggcataaa
     4801 gagctggagg agtataaaaa cgcctcccgg attgatatta aaccgtcacg ggaatatgcc
     4861 agcacaatca tgaacgctat ctggactggc gagccgagtg tgatttacgg caacgtccgt
     4921 aacgatggat tgattgataa cctgccacaa ggatgttgcg tggaagtagc ctgtctggtt
     4981 gatgctaatg gcattcagcc gaccaaagtc ggtacgttac cttcgcatct ggccgccctg
     5041 atgcaaacca acatcaacgt acagacgctg ctgaccgaag ccattcttac ggaaaatcgc
     5101 gaccgtgttt accacgctgc gatgatggac ccgcatactg ccgccgtgct gggcattgat
     5161 gaaatatatg ctcttgttga cgacctgatt gccgcccacg gcgactggct gccaggctgg
     5221 ttgcaccgtt aaggggtaat cgcttccggc gctctggtga ttaagccaaa gcgccgtagc
     5281 tggcggcaat gaaagcgagc gaggaatatc accgtcgtta atgcgccaag cagcgcgcag
     5341 aacatatcgg attgcgtgtc ccactggtcg ccctgggtgc cgagaaaatc atccgctccc
     5401 tgacccattg ccagcgccgc ccaccactcg attaattcat acatcgcgct tatcgccagc
     5461 gccacgcagc agaccagaaa cgccaccatc ttacgtccgc gaacgtacat cccgcgtaca
     5521 aggatctctc gtgccaccag tgcaggcacc agcccctgga aaaaatgccc cagtttgtca
     5581 tacggattac ggctcaaccc cagccattcc tgcacctcaa aaccaacggg gactttcgcg
     5641 taggtgtatt gcccgccgac catcaggatg atggcgtgaa agaaaatgag cgtataaagc
     5701 agcggagtta acggataacg tttagcggtg gcaagcagta gcggcacgac aataatcacc
     5761 ggtgtcactt ccatcagcca ggtgagtttg tcatgggccg aaatgccggt ataaatcagg
     5821 attaacgtta gcgccagtgc gccggtgtta agaattaacg gcttgagtgt gcgggtcatg
     5881 gtgagttcgc aagtcaggga aaatactgac tattgaccag taagcgtgaa attacaatcg
     5941 caaaagaggt ggcccagggg gatcacctgg cagcatgctg ccaggcgctg ggccgaagag
     6001 gttacttagt gcagttcgcg cactgtttgt tgacgatttg ctggaagaag tcgttgcctt
     6061 tgtcatcgac caggataaac gccgggaaat cttctacttc gattttccag atagcttcca
     6121 tacccagctc cggataagcg acgcattcca gatgcttgat gctctgctgc gccagtaccg
     6181 ccgccggacc gccgatacta ccgaggtaga agccgccgtg tttatgacac gcgtcggtaa
     6241 cctgctgact gcggttacct ttcgccagca tgatcatgct gccgccgtgg gattgcagca
     6301 gatccacgta ggagtccata cgacctgcgg tggttgggcc aagtgaacct gatggataac
     6361 cggcaggggt tttcgccgga cccgcgtagt agatcgggtg atctttgatg tactgcggca
     6421 gttctttacc ggcgtcaatc agctctttca gcttggcgtg tgcaatatct cgacccacaa
     6481 taatggtgcc ggtgagcgac aaacgggtgg ataccgggta ttgcgaaagc tgggcgagga
     6541 tctctttcat cgggcggtta aggtcaactt tcaccgcttc gccttcaccg gcctggcgca
     6601 gttcttgtgg aatgtactgg cctgggttgt gttccagttt ttcgatccag ataccttcgc
     6661 ggttgatttt cgctttaatg ttacggtctg cggagcagga gacgcccatg cctaccgggc
     6721 aggatgcgcc gtgacgtggc agacggataa cgcgaatgtc gtgcgcgaag tatttaccgc
     6781 caaactgcgc gccaagaccg agtttctggg cctcttccag cagttcctgt tccagctgga
     6841 catcgcggaa cgcctgacca tgttcgttcc cttccgtcgg cagttcatcg taatagtgag
     6901 cgcttgctaa cttgacggtt ttcaggttgg tttccgcaga cgtaccgcca atcacaaacg
     6961 cgatatggta cggcgggcaa gctgccgtac cgagggtacg cattttctcg acgaggaagt
     7021 ttttcagttt gccgggcgtc agcagggctt tggtttcctg gtagagatac gttttgttgg
     7081 cagagccgcc gcctttagcg acacagagga atttgtactc atcgccatct accgcgtaca
     7141 ggtcgatttg cgcaggcagg ttcgtgccgg tgttgacctc tttatacatg tccagcggcg
     7201 cgttctgtga atagcgcagg ttatcttcga tataggtgtt atagacgcct ttcgacagcg
     7261 cttcttcatc accgccaccg gtccacacgc gctgaccttt tttaccgacg atgatcgcgg
     7321 tgccggtatc ctggcaggtc ggcagcacgc ctttggcggc gatttcggag tttcttaaga
     7381 attgcagcgc cacgtacttg tcgttttcgc tggcttctgg atcgtgaaga atagccgcaa
     7441 cctgtttctg gtgtgctggg cggagcatga aagaagcgtc gtgaaaggct tgctgcgcca
     7501 gcagggtcag ggcttctggt tccactttca ggatggtttc gccgtcgaag tcggcaacgc
     7561 taacgtaatc ggaagtgagt agatagtatt cggtattgtc tttccccatc gggaaaggtg
     7621 cctggtagat aaagggtttg tttgacatag cttccagcct gtaactctgt atttgttatt
     7681 cgaaaatggc gtgccgcgta gtcacgggcg gcacgcaaag tgcatttata agaacccgta
     7741 catcgcggcg aagatccagc cgaagacgca cgatacgctc acaccaatca accccggcag
     7801 aataaagctg tggttgatga cgaagcgacc gatgtgggtg gtgccggaac ggtcaaactg
     7861 aatcgctgcc agatcgctcg gataagtcgg caggatgtaa taaccgtagc aagccggtgc
     7921 tgaagccacg atgtatgccg gatcaacgcc gatcgccagc gcgaccggaa caatcgccgc
     7981 cagcgccgca gcctgagagt ttacaaactt ggaaaccagc agcagaacaa tggcataggc
     8041 ccacggatac tctttcacca tttcacccag tacgccctga atttcagaca tatgcgcacc
     8101 gaacatggtt tctgccatcc atgcgatacc gtacaccgcc acgatggcga tcataccgga
     8161 acggaagact tcgttttttg agatagacgc gggattggtt ttggtcagga taataatcag
     8221 cgccccggtc agcagcataa acatctgaat aaccagtacc atcgacagcg gtttgccgcc
     8281 gaaggatgga cgcaggtccg aatcagcacc aagaagggcg actacagcga ttgccccgag
     8341 gaagatccac attgccagcc agttgctttt cggcagtttt ttatccagca gcgtcgcggt
     8401 atcaccgtaa acatactcac ggttttccgg tacggagatg aatttctgga actcttcgtc
     8461 tttatccaga tctttaccgc ggaaccagct gaagataccg atcgccagga taccgattaa
     8521 cgtcgatgga atggtgattg ccagcaggtc gaggaactca agatggcgac catcaaaggt
     8581 gacattaccc agcatcgcaa ccagagacac gactgcaacc gacaccggac tggcgataat
     8641 ccccatctgt gcaccgatag aacttgccgc cattggacgt tccggacgga tgttgttctt
     8701 aatagcaacg tcgtagatga tcggcagaat ggtgtaaacc acatgacccg taccgcaaag
     8761 aatggtcagc gtacaggtca caaacggcgc gacaattgag acatatttcg ggttgcggcg
     8821 cagcagcttc tcggcaattt gcagcatgac atcaagaccg cccgaagctt gcaaggtcgc
     8881 cgatgccgcc accaccgcaa tgataaccag catgacatca accggtggtt tacctggctg
     8941 aaggtggaag acgaagacca gaatgaccag accgataccg cctaataaac ccagcgcgat
     9001 accacccttt ctggcaccat aaaacagaca tatcagtatt atgataagtt ggatagtaaa
     9061 taacatgtgt gaaccctcgc gataatccta tttaaatttt tgctgaatag atcacagtca
     9121 cgttctgttt tgtatgaact gttttcagag cctgactaaa tatccgtttg gtcgttaccg
     9181 gctttagcaa atacctcaca gtgaatattg gctgattaaa gcagcggcaa atttatgcac
     9241 tgtgataagc ggctttttca gagagaaaaa ctctctggcg atgggtttat agtttgtact
     9301 gcgcagatgt taatgttttg tttgctgtta tcttattgat atttatcggt ctattttttg
     9361 ttctcagggc agatatcatt aggtttatag attaatctga tctacccatt tgtgggt
//
LOCUS       DAZGQL010000156         9356 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.156, whole genome shotgun sequence.
ACCESSION   DAZGQL010000156 DAZGQL010000000
VERSION     DAZGQL010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9356
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.156"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            31..1155
                     /locus_tag="ACHKIR_004738"
     CDS             31..1155
                     /locus_tag="ACHKIR_004738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HFO9992376.1"
                     /translation="MTIHYTATLNNKHLSLTALYPDGNREVIAILPVTELGALINRNN
                     RLMHLSFSSPFREITETVLVDTVDAERPGHHIGRMLSAIDACLDAEYAGKGKHPSRKN
                     GFWRTLMCGFIAGIALPATVAGYHWMKAEPPSTLAYPTSQEWRAEPVTDTETPRPHTE
                     HHVNSIPARPAPTQQEPSSAPAPTAVTEEQHVQAAEARRNLATVLKRNADRGMFTINL
                     SPGHERTLYAFLDPACINCRLLEPALKQLAREFSVVIYPVSVIGGESSTKRTAPLLCE
                     PDSQKRATGWHQLYSADSGMAPTGDAPAAQDGQCLTAARAAIDVNNQAFRQFGFGGTP
                     WVLSDTGWHLPSGILKDATSVRLFLKATDSEEYHNEPAPR"
     gene            1136..3439
                     /gene="trbC"
                     /locus_tag="ACHKIR_004739"
     CDS             1136..3439
                     /gene="trbC"
                     /locus_tag="ACHKIR_004739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugative transfer protein TrbC"
                     /protein_id="HFO9992377.1"
                     /translation="MSQHHVNPDLIHRTAWGNPLWNALHNLNITGLCLAGSIITALIW
                     PLALPVCLLFTLVTSVIFMLQRWRCPLRMPMTLSLDDPSQDRKVRRSLFSFWPTLFQY
                     EADETFPARGIFYVGYRRINDIGRELWLSMDDLTRHVMFFATTGGGKTETTFAWLLNP
                     LCWGRGFTFVDGKAQNDTTRTIWYLSRRFGREDDIEVINFMNGGKSRSEVIQSGDKSR
                     PQSNTWNPFAFSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGK
                     TMSLQMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDMSLVRTPSAWT
                     EEPRKQHAYLSGQFSETFTTFAETFGDVFAADAGDIDIRDSIHSDRILIVMIPALDTS
                     AHTTSALGRMFVTQQSMLLARDLGYRLEGTDAQTLEVKKYKGSFPYICILDEVGAYYT
                     DRIAVEATQVRSLEFSLIMTAQDQERIEGQTSATNTATLMQNAGTKFAGRIVSDDKTA
                     RTVKNAAGEEARARMGSLQRHDGVMGESWVDGNQITIQMESKIDVQDLIRLNAGEFFT
                     VFQGDVVPSASVYIPDSEKSCDSDPVVINRYISVEAPRLERLRRLVPRTVQRRLPTPE
                     HVSSIIGVLTAKPSRKRRKNRTEPYRIIDTFQRQLATAQTSLDLLPQYDTDIESRANE
                     LWKKAVHTINNTTREERRVCYITLNRPEEEHSGPEDIPSVKAILNTLLPLEMLLPVPD
                     LTASPPHKKNVAQTPSGGKQESRKKRF"
     gene            3906..4136
                     /locus_tag="ACHKIR_004740"
     CDS             3906..4136
                     /locus_tag="ACHKIR_004740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001369273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992378.1"
                     /translation="MKTNMGSTKNGARVPHVRNRFTSMDIGMNSQILPDMLINFALIN
                     ITDRKNEGTNTIDGNWQADEGRRYRDNVRIYF"
     gene            complement(4234..4665)
                     /locus_tag="ACHKIR_004741"
     CDS             complement(4234..4665)
                     /locus_tag="ACHKIR_004741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity TolA C-terminal
                     domain-containing protein"
                     /protein_id="HFO9992379.1"
                     /translation="MVLRSIWIEEQYMGVKKIVMLAILVSFVAGCAPLHPSNCHKTTA
                     LGSCSSGRWDDQDEWGEQARGIRDAINAKLDDPQKWKRKKCRLHMEFSRDGTALKIST
                     SNGDKAYCEAIKSAAHKAKFPAFNNPEVYRDFQKSGFDMRG"
     gene            complement(4733..7444)
                     /locus_tag="ACHKIR_004742"
     CDS             complement(4733..7444)
                     /locus_tag="ACHKIR_004742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFO9992380.1"
                     /translation="MNAIIPHKRRDGRSSFEDLIAYTSVRDDMQENELTEDSEIKSDV
                     PHRNRFRRLVDYATRLRNEKFVSLIDVMKDGSQWVNFYGVTCFHNCNSLETAAEEMQY
                     KADKAVFSRNDTDPVFHYILSWPAHESPRPEQLFDCVRHTLKSLELSKHQYVAAVHTD
                     TDNLHVHVAVNRVHPVSGHINRLSWSQERLSRACRELELKHGFAPDNGCFVHAPGNRI
                     VRKTALVRERRNAWRRGKKQTFREYIAQMSIAGLREEPAQDWLSLHKRLASDGLYITM
                     QEGELVVKDGWDRAREGVALSSFGPSWTAEKLGRKLGEYQPVPTDIFSQVGTPGRYEP
                     EAINVDIRPEKVAETESLKQYACRHFAERLPEMARNGELESCLDVHRTLAKAGLWMGI
                     QHGHLVLHDGFDKQQTPVRADSVWPLMTLDYMQDLDGGWQPVPKDIFTQVIPGERFRG
                     RNIGTQAVSDYEWYRMRMGTGPQGAIKRELFSDKESLWGYTTVQCESLIEDMIAGGNF
                     SWQACHEMFARKGLMLQKQHHGLVIVDAFNHELTPVKASSIHPDLTLSRAEPQAGPFE
                     IAGADIFERVKPECRYNPELAASDEVEPGFRRDPVLRRERREARAAAREDLRARYLAW
                     KEHWRKPDLRYGERLREIHAACRRRKAYIRVQFRDPQLRKLHYHIAEVQRMQALIRLK
                     ESVKEERLSLIAEGKWYPLSYRQWVEQQAVQGDRAALSQLRGWDYRDRRKDKRRTTNA
                     DRCVILCEPGGTPLYEDTGVLEARLQKDGSVRFRDRRNGELVCVDYGDRVVFYHHQDR
                     NELVDKLNLIAPVLFDREPGMGFEPEGSYQQFNDVFAEMVAWHNAAGITGNGHFVISR
                     PDVDLHRQRSEQYYHEYIRQQKSISGGHGASYAPVQDNEWTPPSPGM"
     gene            complement(7456..7788)
                     /gene="mobA"
                     /locus_tag="ACHKIR_004743"
     CDS             complement(7456..7788)
                     /gene="mobA"
                     /locus_tag="ACHKIR_004743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="HFO9992381.1"
                     /translation="MSEKKTRTGSENRKRIVKFTARFTEDEAEIVREKAESSGQTVST
                     FIRSSSLDKVVNCRTDDRMIDEIMRLGRLQKHLFVEGKRTGDKEYAEVLVAITEYVNA
                     LRRDLMGR"
     gene            8021..8356
                     /locus_tag="ACHKIR_004744"
     CDS             8021..8356
                     /locus_tag="ACHKIR_004744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobC"
                     /protein_id="HFO9992382.1"
                     /translation="MASKKFYSDDDIQLAKAALSELPDLTAQRKTLRDFLDAIRDDII
                     VLVRTKGYTLADVRDTLKNAGYEVGEKALRDIIREAESKKPSRRSSSKRAPKKTDSGR
                     NDGIDMNNN"
     gene            complement(8441..9289)
                     /locus_tag="ACHKIR_004745"
     CDS             complement(8441..9289)
                     /locus_tag="ACHKIR_004745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFO9992383.1"
                     /translation="MNQTLPTADLNTDGTTDVIPSVAIDRIIAQRNEGISLFMQAMEC
                     LATARRILLDASGDIFLYGFEDCVTDSVRCMDKPEEAKKNITRLADRKIWDRLMTDTG
                     MYTFMSSCQRDEWNSQLMSDTCPEITLDNVLATFRHLNASKMQTFEHGLIDVYRKLSW
                     DYRTNNPCRLGKKIIIENLLYRWSNGRVTLDCSGREALDDLVRPFYLLEGRNVPDFRN
                     SIGAQYGEFLGNGDNVGELFEGEYFTVRGYQKGTVHIVFKRPDLVEKLNDIIARHYPG
                     ALPPRV"
BASE COUNT         2373 a         2599 c         2139 g         2245 t
ORIGIN      
        1 acagcatccc gccgacaagg aaaaagaaca atgaccatcc actacactgc aacactgaat
       61 aataaacacc tgtccctgac cgccctgtac cctgacggta atcgggaggt gattgccatc
      121 ctgccagtca cagaactggg cgcactgatt aaccgtaata acaggctgat gcacctgtcc
      181 ttcagttccc cttttcgcga aataaccgaa acggtgcttg ttgataccgt tgatgcagaa
      241 cgtcccggac atcacatcgg cagaatgctc tcagcgattg atgcctgcct ggatgccgaa
      301 tatgccggaa aaggaaaaca tccctcccgg aaaaatggct tctggcggac actgatgtgt
      361 ggctttatcg ccggaattgc tctgccggcg acagttgcag gttaccactg gatgaaggca
      421 gagccccctt ccacgctggc ttaccctaca tctcaggaat ggagagctga accagtaacc
      481 gacacagaga caccacgccc acatacggaa catcatgtta acagcatccc ggcccgcccg
      541 gctcccacac agcaggaacc ttcttctgcc cccgccccga cggcagtgac ggaagaacaa
      601 cacgttcagg ccgctgaggc ccgccgtaat ctggccacag tacttaaacg caacgcagac
      661 cgggggatgt tcaccattaa cctgtcccct ggtcatgaac gcacgctgta cgccttcctt
      721 gaccccgcat gcatcaactg ccgtctgctg gaacccgcgc ttaagcaact ggcccgggaa
      781 ttcagcgtgg tgatttatcc ggtctccgtg attggcgggg agtccagtac aaagcggact
      841 gctccgctgc tgtgtgaacc ggactcacaa aaacgggcga ccggatggca tcagctgtat
      901 tccgctgaca gtggtatggc ccccaccggc gatgcaccgg ccgcacagga tggacagtgt
      961 ctgaccgccg cacgtgctgc catcgatgtc aataaccagg ctttccgcca gttcggtttt
     1021 ggcggaaccc cctgggtgtt atccgatacc ggctggcacc tgccgtccgg catcctgaaa
     1081 gacgccactt ccgttcgcct cttcctgaaa gcaacagata gcgaggagta tcacaatgag
     1141 ccagcaccac gttaatcctg acctcataca ccgcacggcc tggggaaacc cgttgtggaa
     1201 tgcactgcat aacctgaaca tcactggtct gtgtctggcc ggcagtatca ttacggcgct
     1261 gatatggcca ctggcccttc ctgtctgcct gctgttcacc ctggtcacct ccgtgatatt
     1321 catgctgcaa cgctggcgat gccccctgcg tatgcccatg acactcagcc ttgatgaccc
     1381 ctcccaggac agaaaggtca ggagaagcct cttcagcttc tggcccaccc tgtttcagta
     1441 tgaagcggat gaaacattcc ccgcccgggg gattttttat gtcggttacc gccgtatcaa
     1501 cgatatcggt cgtgagctct ggctgagcat ggatgacctc acccggcatg tcatgttttt
     1561 tgccaccacc ggtggcggta aaacagaaac cacctttgcc tggctgctca acccgctctg
     1621 ctggggaaga ggctttacct ttgttgacgg caaggcacag aacgatacca cgaggacaat
     1681 ctggtatctc tcccgacgtt ttggcaggga ggatgatatc gaagtcatca acttcatgaa
     1741 cggcggaaaa tcccgcagtg aagtcattca gagtggcgat aaatcccgcc cacagtcaaa
     1801 cacctggaac ccgttcgcat tcagcaccga agccttcacc gccgaaacca tgcagtccat
     1861 gctgccccag aacgttcagg gcggtgaatg gcagtcacgg gcgatagcca tgaacaaggc
     1921 actggtcttt ggcacaaaat tctggtgcgt ccgggaaggt aaaaccatgt ccctgcagat
     1981 gttacgggaa catatgacgc tggaagggat ggcaaaactc tattgccgtg gccttgatga
     2041 ccagtggccg gaagaagcca tcgcaccact gcgaaactac ctgcaggatg tcccgggatt
     2101 cgatatgtca ctggtgagaa cgccgtcagc ctggactgag gagccccgta aacagcatgc
     2161 ttatctctcc ggtcagttct cggaaacgtt cacaaccttt gccgaaacct tcggggacgt
     2221 ctttgcggca gatgccggcg acatcgatat tcgcgacagt attcacagcg accgcattct
     2281 gattgtgatg atccctgcac tggacacatc ggctcatacc acgtcggcac tcggtcgtat
     2341 gttcgtcacc cagcagagca tgctcctcgc ccgtgacctg ggctaccggc tggaaggcac
     2401 agatgcacag accctggaag tcaaaaaata caaaggcagc ttcccctata tctgtatcct
     2461 tgacgaagtc ggtgcctact acacagaccg cattgctgtg gaggccaccc aggtacgttc
     2521 actggagttt tccctgataa tgacggcgca ggatcaggag cgaattgaag ggcagacctc
     2581 cgccaccaac accgccacac tgatgcagaa cgccggcacc aaattcgccg gacgcattgt
     2641 cagtgacgac aaaacggcaa gaacggtgaa aaatgccgcc ggtgaagagg cacgggccag
     2701 aatgggcagc ctgcagcgcc acgatggtgt gatgggtgag tcctgggtgg acggtaacca
     2761 gatcaccatc cagatggaaa gcaaaatcga cgtgcaggat ttgatcagac tgaatgctgg
     2821 tgagttcttt accgttttcc agggggatgt ggtgccgtca gcctctgtgt atattccgga
     2881 cagcgagaaa agttgcgaca gtgatccggt ggtcatcaac cgctatattt ccgttgaagc
     2941 cccccggctg gagcgtctcc gccgtctcgt ccccaggaca gtgcagcgca ggctccccac
     3001 accggagcat gtcagcagca tcatcggtgt actgacagca aaaccatccc gtaaacgtcg
     3061 taaaaaccgt acagagcctt accgtatcat tgatacattc cagcgccagt tggcaacagc
     3121 gcagacatca ctggatctgc ttccgcaata tgatacagat atcgaatccc gggcaaacga
     3181 actgtggaaa aaggctgtgc acactatcaa taacacaacc agggaagaac gccgggtatg
     3241 ttatatcaca ctgaatcgtc cggaagagga acactccggt cctgaagata taccttcagt
     3301 gaaggcgatt ctgaatactc tgctccccct ggagatgctg ttacctgtac cggatctaac
     3361 tgcatccccg ccccataaaa aaaatgtcgc tcagacacca tcaggtggaa aacaagagag
     3421 tcgtaaaaaa agattctgat aacacctgta tggctgattt taataacaac ctttacgcga
     3481 ttattaatct cctccgctca ccatagtaag cggaggattc aggatttcaa tccatcacca
     3541 gaggtacaat aaatgtgtat tagtagcccg gaaacaaact catggtctgt tatttatcgt
     3601 aaaaattccg gagaagatat caatatcact tccctgacgt ttaaaaatag tcttctggca
     3661 gcccggacac tgatggtgcc cgaaaactat atgatatgca tactcagaaa cggggagagg
     3721 gtcagacgat gggacagaga aatacttgca ggctcaaaca gatggtacaa gtgttctccc
     3781 gataatttcg aaatactggg taagctcccc ataataaata aagtcacaaa actgataaaa
     3841 tcataatcta cggattttaa tcagttaagc taaatcagga tgaacgcttt gattatgaat
     3901 tatacatgaa aacaaatatg ggcagcacaa aaaatggtgc cagggttccc cacgttcgta
     3961 atagattcac ttctatggat attggtatga attcccagat cctgccagac atgctgatta
     4021 attttgccct tattaacatc acagacagaa agaacgaggg taccaatacc attgatggta
     4081 actggcaggc cgatgaagga cgccgttacc gggataatgt aagaatctat ttttgagtat
     4141 gtcaacataa cctgaggcaa agtacctcag gttccagtgt agtaatcagc caacctgaca
     4201 gtcttctgtt agccctacag atatgtcgtg gagctaaccg cgcatgtcaa agccggattt
     4261 ttgaaaatct ctataaactt ccggattgtt gaaggccggg aatttagctt tatgggctgc
     4321 tgactttatc gcttcgcaat aggctttatc gccattactg gttgatattt ttaacgccgt
     4381 gccatcccgg gagaattcca tatgcaacct gcattttttc cttttccatt tttgcggatc
     4441 atcaagttta gcattaattg catccctgat tccccgtgct tgctcccccc attcatcctg
     4501 atcatcccaa cgtcctgaac tgcaactacc cagggctgta gttttatggc aatttgaagg
     4561 atgtaatggc gcacatcccg ccacaaagct gactaaaata gccagcataa cgattttctt
     4621 tacccccata tattgctcct caatccatat agatctcagt accatagctc tgtcagagca
     4681 aagaaaatct taactaatac aagttaagat ttttaatatc cactcctcat ccctacattc
     4741 caggtgatgg tggcgtccac tcattatcct gaactggagc ataagatgcg ccatgccctc
     4801 cggatatgct tttctgttgc ctgatatatt cgtggtagta ctgttcacta cgctgacgat
     4861 gtaaatccac atccgggcgg ctgattacaa agtgtccatt tccggttatt ccagctgcat
     4921 tatgccaggc caccatttcc gcaaatacat cattaaactg ttgatatgag ccttccggtt
     4981 caaaacccat tcctggctca cgatcaaaca atacaggagc aatcagattc agcttatcca
     5041 ccagttcatt tctgtcctga tgatggtaga acaccacacg gtccccataa tccacacaga
     5101 ctaactcgcc attcctccga tcacgaaaac gcacactgcc atctttctgc agacgggctt
     5161 cgagaacgcc agtgtcttca taaagtggag ttcctcccgg ttcacagaga atcacgcagc
     5221 ggtcggcatt cgttgttcgt cgcttgtctt tgcgacgatc acgataatcc cagccacgta
     5281 actgtgataa tgcagctctg tctccctgta ccgcctgttg ctccacccac tgccggtagg
     5341 acaaaggata ccacttaccc tccgcaatca gtgacaaccg ctcttccttc acactctcct
     5401 tcagcctgat cagcgcctgc atacgctgca cttctgcaat atggtaatgc agcttacgca
     5461 attgtggatc ccggaactgc acacggatat acgccttgcg ccgacggcag gcagcatgaa
     5521 tttcccgtaa ccgttctccg taacgtaagt cgggtttacg ccagtgctcc ttccatgcaa
     5581 gataacgcgc gcgcaaatct tcccgcgcag ctgcacgggc ttcacggcgt tcacgacgca
     5641 gtacaggatc gcgccggaag ccgggctcca cttcatcgct ggcagcaagt tccggattgt
     5701 agcgacattc aggcttcacc cgttcaaata tatctgcacc tgcaatctca aatggccctg
     5761 cctgcggctc agccctggat aacgtcaaat ccgggtggat actgctggcc ttcactggtg
     5821 tgagctcatg attaaacgca tccacaatca caagaccatg atgctgtttc tgcaacataa
     5881 gccctttacg cgcaaacatc tcatggcacg cctgccagct gaaattcccg cccgcgatca
     5941 tatcttcaat caacgactca cactgaacag tcgtgtaccc ccacagactt tccttatcag
     6001 aaaacagttc gcgctttatt gctccctgtg gacctgtccc catccgcata cgataccatt
     6061 cgtaatcact gacagcctga gtaccaatat tacgtccccg gaatcgttct ccgggtataa
     6121 cctgagtaaa aatatccttc ggtacaggct gccatccacc atccagatcc tgcatataat
     6181 ccagcgtcat taacggccac acactgtctg cacgtactgg tgtctgttgt ttatcaaatc
     6241 catcatgcag taccagatgc ccgtgctgga tccccatcca taatccagcc tttgccagag
     6301 ttctgtgcac atccaggcaa ctttccagct caccatttct cgccatttcc ggtaagcgtt
     6361 cagcaaagtg acgacaggcg tactgtttca gactctccgt ttctgccact ttttccggcc
     6421 ggatatccac gttaatggct tccggctcat aacgaccggg tgtccccacc tgactgaaaa
     6481 tatctgtcgg tacaggctga tattccccca gtttccgccc cagtttttca gctgtccatg
     6541 acggtccgaa cgaactgagt gcaacacctt cccgcgctct gtcccagcca tccttcacca
     6601 ccagttcccc ttcctgcatc gtgatataca gaccatcact ggccagccgt ttatgcaaag
     6661 ataaccagtc ctgtgcaggc tcttcacgca gcccggcaat cgacatctga gcaatatatt
     6721 ccctgaacgt ctgctttttc ccgcgtcgcc aggcattacg gcgctcacgt accagcgctg
     6781 ttttgcgcac aatacggttg ccgggtgcat gcacaaagca gccattatca ggcgcgaatc
     6841 cgtgcttaag ttcaagttca cgacatgcac ggctgagcct ttcctgactc caggaaagac
     6901 gattaatatg accactaaca ggatgaacac gattcactgc gacatgtaca tgcaggttat
     6961 ccgtgtccgt atgaacggca gccacgtact gatgttttga gagttcaagg gatttcaatg
     7021 tgtgacgcac acagtcaaaa agctgctcag gacgcgggct ttcatgcgca ggccaggaga
     7081 gaatgtagtg gaaaacgggg tccgtatcat tacgggaaaa aacagctttg tccgccttgt
     7141 actgcatctc ttcagcagct gtttccagtg aattgcagtt atggaaacag gtaacgccat
     7201 agaaatttac ccactgactg ccgtctttca tcacgtcaat cagggagaca aatttttcgt
     7261 tacgcaggcg tgtagcatag tcgacaaggc gtctgaaacg gttgcggtga gggacgtcac
     7321 tcttgatttc gctgtcttct gtgagctcat tttcctgcat atcatcccgc acagatgtgt
     7381 acgcaatcag gtcttcaaac gaagacctgc cgtcacgtct tttgtgcgga ataatggcat
     7441 tcatcagcag ttatctcagc gtcccattaa gtctcttcgc agagcattga catattcagt
     7501 aatggcaaca agaacctcgg catactcctt atcaccagta cgttttcctt ccacaaacag
     7561 atgcttctga aggcggccaa gacgcataat ctcatcaatc atacggtcgt cagtacggca
     7621 gttaaccacc ttatccagtg aagagcttcg tataaatgtt gaaaccgtct gtccacttga
     7681 ctccgctttt tctctgacga tttcagcctc atcctccgtg aatctcgccg tgaattttac
     7741 aattctcttc ctgttctcac tgcccgttcg ggttttcttc tcactcatgg tctgctcctt
     7801 accggggtgt cggggcgaag ccctgaccag gaggcatttg tctgattgcg cgtgcgcggt
     7861 ccgacaaatg cacatcctgt cccgtttttc aggacattat agcctgttat ttttgtgcat
     7921 catagtctta tgcagacggc agaggcaggg cgcagccctg ccaggagtca gtttttgatg
     7981 ataaaatcag gcttcaacat gaaacagaga gaaagtgcca atggcttcta aaaaatttta
     8041 ctcagatgat gacatccagc tggcaaaagc tgctttatcg gaattgccag atctcaccgc
     8101 tcaacggaaa acattgcgtg attttcttga tgccattcgt gatgacatta ttgtcctggt
     8161 aaggacaaag ggttatacac tggcagatgt tcgtgacacg ctcaaaaatg cagggtatga
     8221 agtaggagag aaggcacttc gtgacattat tcgggaagca gaatcgaaaa aaccatcccg
     8281 ccgttcttcc tctaaaagag cacctaaaaa gacagattca ggcaggaatg atggcatcga
     8341 tatgaacaac aattgatgtg acaatgtcac atcgtgttct gagattatga tgattacaga
     8401 aaaaaagccc cggcataaat accagggctt ttctgtttaa tcagactcgt ggcggcaatg
     8461 caccaggata atgccgtgca ataatatcat tcagtttttc aacaaggtca ggacgcttaa
     8521 agacaatgtg tacggtccct ttctggtagc cacgcaccgt aaaatattcc ccttcaaaca
     8581 actcaccgac attgtcgccg ttcccgagaa attcaccata ctgcgccccg atgctgttcc
     8641 tgaagtcagg aacgttgcgc ccctccagca gataaaacgg acgtaccagg tcatccagtg
     8701 cctcccgtcc gctgcagtcc agcgtcacac gcccgttact ccagcggtac agcaggtttt
     8761 caataatgat tttctttccc agacggcagg gattattggt tctgtaatcc catgacaatt
     8821 tccggtagac atcaatcagt ccatgttcaa atgtctgcat cttactggca ttcagatggc
     8881 gaaaagttgc cagtacattg tccagggtga tttcaggaca ggtgtcgctc atcagctggc
     8941 tgttccactc atcacgctgg catgaactca tgaatgtgta catgccagta tccgtcatca
     9001 ggcgatccca gattttccgg tcggcaagac gggtgatatt cttttttgct tcttccggtt
     9061 tatccatgca tcgaacggag tcagtcacgc agtcttcaaa cccgtagaga aaaatgtcac
     9121 ctgacgcatc aagcagaatc ctgcgtgctg tcgccaggca ttccatcgcc tgcatgaaca
     9181 gtgaaatacc ttcgttacgc tgcgcgatga tgcggtcgat agccacagac ggaataacat
     9241 ctgtcgtgcc gtcagtattc aggtcagcag tgggtaaagt ctgattcatc acattgtctc
     9301 ctcatcgtca cgactgccgg gtcagccagg tcatttcccg gaggagcgaa gctcct
//
LOCUS       DAZGQL010000157         9111 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.157, whole genome shotgun sequence.
ACCESSION   DAZGQL010000157 DAZGQL010000000
VERSION     DAZGQL010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9111
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.157"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..2023
                     /gene="ireA"
                     /locus_tag="ACHKIR_004746"
     CDS             <1..2023
                     /gene="ireA"
                     /locus_tag="ACHKIR_004746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001534707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor IreA"
                     /protein_id="HFO9992384.1"
                     /translation="GIAVICSTVISSGYASSDKKEDTLVVTASGFTQQLRNAPASVSV
                     ITSEQLQKKPVSDLVDAVKDVEGISITGGNEKPDISIRGLSGDYTLILVDGRRQSGRE
                     SRPNGSGGFEAGFIPPVEAIERIEVIRGPMSSLYGSDAIGGVINIITKPVNNQTWDGV
                     LGLGGIIQEHGKFGNSTTNDFYLSGPLVKDKLGLQLYGGMNYRKEDSISQGTPAKDNK
                     NITATLQFTPTESQKFVFEYGKNNQEHTLTPGDSLDAWTMRGNLKQPNSKRETHNSRS
                     HWVAAWNAQGEILHPEIAVYQEKVIREVKSGKKDKYNHWDLNYESRKPEITNTIIDAK
                     VTAFLPENVLTIGGQFQHAELRDDSATGKKTTETQSVSVKQKAVFIENEYAATDSLAL
                     TGGLRLDNHEIYGSYWNPRLYAVYNLTDNLTLKGGIAKAFRAPSIREVSPGFGTLTQG
                     GASIMYGNRDLKPETSVTEEIGIIYNNDSGFSASATLFNTDFKNKLTSYDIGTKDPVT
                     GLNTFIYDNVGEANIRGVELATQIPVYDKWRVSANYTFTDSRRESDDESLNGKSLKGE
                     PLERTPRHAANAKLEWDYTQDITFYSSLNYTGKQIWAAQRNGAKVPRVRNGFTSMDIG
                     MNYQFLPGTLINFAVLNVTDRKSEDIDTIDGNWQVDEGRRYWANVRISF"
     gene            2689..7005
                     /locus_tag="ACHKIR_004747"
     CDS             2689..7005
                     /locus_tag="ACHKIR_004747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFO9992385.1"
                     /translation="MNLRKKHICIAISLVLNPFPASLAAQNTNVVEVDESSQGYDVHG
                     DNWKSIEIGTTQEVKASPDKNINFDHNSPVAEGRNSIAIGTSAEAGKEMTDKMAGEAI
                     AIGAKAKATKEQSIAIGGDTKATGWGAIVIGGDDLTPLHGDRFGDKEIPNNYSSSEAS
                     GDGSIVVGSRSTAAGDLSVSLGSISSANGTAANALGATAEANGDMATAVGFNALANAS
                     NALAVGSQASADGEDSLAIGSQSTTGKKSTVALGQGATASAAEGNVAIGADSVDKAAT
                     PVSGATIKLKNGGTIEYKGFAGDKAASVVSVGDAGKERQIVNVGAGAISDTSTDAING
                     SQLYAIAKTLKDDLDAINPTWTLKDDKGGSATINGGKDNKNGNTLTVKGDGDIAVTVN
                     DKGMTIDGTTLKNEIKNDAIKIKGGDGKTLTKSLGDNVEITGKGPIKTQVAGDKLEIS
                     AETASLPVNGGNGKITVNNGDGDKLVTAKNIAEAINQSGWKIKADKDTGGVIEGNGQG
                     TETLIKPGDMVTLKAGKNLSVKQSGGEFTFSVIDTPSFSSVQLNGNGKNGNVTLSSEG
                     NKLTLSGGNGAGQNGVVISGVASGLEGKELSTLTNTDKEMKNAASVADLKTAIDGVST
                     SMQGAGFALKDSKGQEVKQSLGQSIKVSGDSNITTTAITNGDKGLKISLNNNLSVGSN
                     NTPGSIKVNGADGKEAVSLNGQDGTIKLTSAPAANGKSASATIAVNAGKGGLTDAAGT
                     TKTRLTYAPDGTLPAEELATMNDGLSFEGDDGKVIHKKLNEKLTIKGGAGDNEDVTDS
                     NIRVDANEKGDGLLVRMTRNLRDLASAVFGTGGQITTVNDKGITITTPAAGGGQGNTV
                     SLTGSGLNNGGNQITSVASGLNGTALADAGGDVLNNAANIGDLKNALRDNSAALVNTG
                     FGLQADDGSKVNTSLGKHITVGGDGQNISTTVQNGSLTVRLADNIRLADDGSVSVGGT
                     RMDKNGLTIKDGPSVTVNGIDAAGRVISGVKDGVNDQDAVNVSQLNREISQATAATTW
                     ALKTESGDEAVAVSSQTVEVRHGQNTRVSAISKDDKGNYSYEIDVTGIPVEYTDSQGN
                     PLVNIGGRFYTQTEDAATGKLTMTPAEPARVRISSEQPLVLTNVAAGNVSPVSTDAVN
                     GSQLASAARIAGGDNVVWKDGKAALAPDTFSELTTAGGGKTTVNGGVHKTPDNVADAV
                     NMLNREGTKYFKAASSGQAAKAEGRDSIAVGQGAVSRGQNSIAMGNGAEVTRQAAAGS
                     VAVGSHARAGRANTGTYALNSQAVAGRTGSDTAVVSFGQPGQERQLQSVAPGVLSANS
                     TDAVNGSQLHATNRQVAGNTQAINTLGNKFSQLSYRVEELNRDIRGVGASAAAMSGIP
                     QAYLPGKSLMGLGVGGYGGESAIAIGVSRISDNGKMIMKLNAGQNTRGNFSVGAGVGW
                     QW"
     gene            7030..7803
                     /locus_tag="ACHKIR_004748"
     CDS             7030..7803
                     /locus_tag="ACHKIR_004748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFO9992386.1"
                     /translation="MLFSRMMMTGVLSLMLAGCGNLSHVTDEGTTEEVVWPSPEKVMF
                     NHDGTQKGSWPLPEKLRQIKAGMNKDQIYQLAGRPHFAEGMSGVREWDYLFNFTGPEG
                     DYTCQYKILFDRNMDARSFYWKPENCLPEAETAEYRESVPGDILFDFDSWTLTERGRD
                     ALSRVAEKFQAGHVVGVKIEGFTDHLGGQAYNRLLSEKRAESVRGWFIARGFRAERIH
                     ATGRGYDAPVVFCPDVEGEALKECLRPNRRVEITGQSVR"
     gene            7954..8779
                     /locus_tag="ACHKIR_004749"
                     /pseudo
     CDS             7954..8779
                     /locus_tag="ACHKIR_004749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         2630 a         1814 c         2439 g         2228 t
ORIGIN      
        1 gggcattgcc gtgatatgtt ctacagtcat atcatcaggt tatgccagtt ctgataaaaa
       61 agaagatacg cttgttgtta ctgcctccgg cttcactcag caactcagaa atgcaccggc
      121 cagtgtttca gtcattactt cagagcaact gcaaaaaaaa ccggtttcag acctggtcga
      181 tgcggtaaaa gatgttgaag ggattagtat caccggtggg aatgaaaaac cggatatcag
      241 tatacgtggg ttaagtggcg attatacatt gattctggtc gatggacgac gtcagagtgg
      301 tcgggaatcc agaccaaacg gcagcggcgg ttttgaagcc ggatttatcc ctcctgtgga
      361 agcaattgag cgtattgaag ttatacgtgg ccctatgtct tccctgtatg gttccgatgc
      421 tatcggagga gttattaata tcataaccaa acctgttaat aaccagacat gggatggcgt
      481 acttgggctt gggggaatta ttcaggaaca tgggaaattt ggtaactcaa ccacgaatga
      541 cttctacctg tcaggcccat tggttaagga taaacttggt cttcagttat atggaggaat
      601 gaactatcgc aaggaagata gtatctctca gggaacacca gcaaaagata ataagaatat
      661 aacggcaaca ctccagttta ctccgactga aagccagaag tttgtttttg aatacggaaa
      721 aaataaccag gaacatacat taacacctgg tgattctctt gatgcctgga ctatgcgggg
      781 aaatcttaaa caaccaaaca gtaaaagaga aacacataac tcacgtagtc actgggtagc
      841 agcatggaat gcccagggcg aaatactgca tcctgaaatt gctgtttatc aggagaaagt
      901 tattcgtgag gttaaatctg gtaaaaaaga taaatataat cattgggatc ttaattacga
      961 gtcaagaaaa ccggaaataa ccaacacgat catagatgca aaagtgacgg catttctgcc
     1021 ggaaaatgtg ctgaccatcg gaggtcaatt tcagcatgca gaactccgtg atgactcagc
     1081 cacaggtaaa aaaacgacag aaacacagtc tgtttcagtt aaacagaaag ctgtttttat
     1141 agaaaatgaa tatgctgcaa cggattctct cgccctgact ggagggctgc gcctcgataa
     1201 ccatgaaatc tatggcagtt actggaatcc aagattgtat gctgtttata acctgaccga
     1261 taatctcact ctcaaaggag ggatcgcaaa agcatttcgg gccccttcga ttcgtgaggt
     1321 cagtcctgga tttggaacac tgacgcaggg tggtgcctct attatgtatg gaaacaggga
     1381 cctgaaaccg gagaccagtg taaccgaaga gatcggtatt atttataata atgacagtgg
     1441 tttttcagca agcgcgactc tgtttaatac agattttaaa aataagctga ccagttacga
     1501 tataggcaca aaagatccag tcactgggct aaacactttt atttatgata atgtgggtga
     1561 ggcaaatatc agaggtgtgg agcttgcaac tcagattcct gtgtatgata aatggcgtgt
     1621 atctgcaaac tatacattca ctgactcccg tcgagaaagc gatgacgaaa gtctcaatgg
     1681 caagtcgctg aaaggggaac ctctggaaag aactcccaga catgcagcca atgcaaaact
     1741 ggaatgggat tacactcagg atattacatt ttattcatct ctgaattata caggaaaaca
     1801 aatctgggca gcacaaagaa atggtgccaa ggttccccgt gttcgtaatg gattcacttc
     1861 tatggatatt ggtatgaatt accagttcct gccaggcacg ctgattaatt ttgccgtact
     1921 taacgtcaca gacagaaaga gcgaagatat cgataccatt gatggtaact ggcaggtcga
     1981 tgaaggacgt cgttactggg ctaatgtaag aatatccttc tgagagaata tagataacct
     2041 gaggccgtgc gcctcaggta ccagtatagt aatcagtaac gtcagcttcc aggttgtaat
     2101 gcttttaatt ttgataaaaa taaacttaat aaattacatt tgttaaatat tgataatttt
     2161 aatttggttt tattactcca gttatactca cactctatct ttacacaatt catcaccatt
     2221 acacctccat agcctgttga tttaaaagat ttaatttcct gtgaaacaat caggcctgta
     2281 tgaacctgat tataccacat aatcttttaa cttatttaaa tttaactctc aggcttttct
     2341 gtcagtaaaa tcagaaagaa ttgcaggatt tcgttttttt gctgcgaagt gaaatgtatc
     2401 tcatttttac tgccttcgcc agtgcgtcag gcaatggcag gtagtgtacc tgcattctgc
     2461 tgtaacgcgt aatgtcgggt attttactta atatgtttta tgtttgcatt attataaatc
     2521 actttattgc cttttgtctg ctttttaatg gttcctgttt ttttacttct ggctttatat
     2581 aaatggatgc tgaagaaatg aaggcagtcc accttacata atattcatgc atgattttta
     2641 tgctattccg actgagtgga agaaaataaa ggagaaaaga ggatatatat gaacctcaga
     2701 aaaaaacata tctgtatagc catatcactt gttcttaatc cgttccctgc atcactggca
     2761 gcacaaaaca ctaatgtagt tgaagttgat gaaagcagtc aggggtatga tgtgcatgga
     2821 gataactgga agagcattga aatagggaca acgcaggaag ttaaagcatc ccccgataaa
     2881 aatattaatt tcgatcataa ttctcctgtt gcagaaggac gtaactctat cgcgattggt
     2941 accagtgcag aggctggtaa agaaatgacg gataaaatgg caggggaagc catagctatt
     3001 ggtgccaagg cgaaagcaac taaagaacag tcaattgcaa taggtggcga taccaaagct
     3061 accggatggg gggctattgt tattggtgga gatgatttaa cccctttgca tggtgacaga
     3121 ttcggtgata aagaaattcc gaataattac tcgtcatctg aagcatcagg agacggaagt
     3181 attgttgtgg gctcaaggag cacagctgca ggagacctgt ctgtgtccct gggctcaata
     3241 agctctgcca acgggacggc tgctaatgca ctgggagcca cggcggaagc taacggggat
     3301 atggcgactg cggttggttt taatgcgctg gctaatgcca gtaatgctct ggctgttggt
     3361 agtcaggcct ctgccgacgg agaggattcc ctggctatag ggagtcagag tacgacgggc
     3421 aaaaagtcca cggttgctct ggggcaggga gctacagcct ctgccgcgga ggggaacgtg
     3481 gccatcggtg ctgattcagt ggacaaggca gcaactccgg tttccggcgc taccattaag
     3541 ttaaagaatg gcgggactat tgaatacaag ggctttgccg gagataaggc tgcatcagta
     3601 gtatctgttg gtgatgcggg taaagagcgt cagattgtta acgtgggggc gggtgcgatt
     3661 tctgacacaa gtacagatgc cattaacggt agtcagttat atgcgatagc gaagacactg
     3721 aaagatgacc ttgatgccat taatccgacg tggacgctaa aggatgataa gggaggttct
     3781 gcgactatta atggtgggaa ggataataag aatggtaaca ctttaactgt taaaggtgat
     3841 ggagatattg cagttactgt taatgataaa ggaatgacga ttgacggaac aacgctgaaa
     3901 aatgaaataa aaaatgatgc catcaaaatt aaaggtggag atggtaagac gctgacgaag
     3961 agtcttggtg acaatgtcga aatcactggc aaaggcccaa taaaaaccca ggtggctgga
     4021 gataaactgg agatttcagc agagacagct tcgttgcctg taaatggtgg taacgggaag
     4081 attacagtaa acaatggtga tggcgataaa cttgttactg cgaaaaacat tgctgaagcc
     4141 ataaaccagt ctggctggaa aattaaagcg gataaagata ccggtggtgt tatcgaggga
     4201 aatggtcagg gaacagaaac actgataaaa cccggtgata tggttacgct gaaagccggt
     4261 aaaaatctca gcgtgaaaca aagtggtggt gaatttacat tctctgttat tgatactccg
     4321 tcattcagca gtgttcagct gaacggtaat ggaaaaaacg gaaatgtaac gctctcatcc
     4381 gaaggtaata agctgacatt gtcaggcggt aatggtgccg gacaaaatgg tgttgttatc
     4441 agtggtgtgg caagtggact ggaaggtaag gaactttcga cgttaacgaa tactgataaa
     4501 gagatgaaaa atgccgccag tgttgcagac ctgaaaacgg caattgatgg tgtgtccacc
     4561 agtatgcagg gagcaggttt cgcactgaaa gattctaagg gacaggaagt gaaacagtct
     4621 cttgggcagt cgattaaagt atccggggac agcaatatca ccaccacagc catcaccaat
     4681 ggggataagg ggttaaaaat atccctgaat aataacctga gcgttgggag caataatact
     4741 cccggcagca tcaaagttaa cggggctgat ggtaaggagg ctgtgagtct gaatggtcag
     4801 gatggcacga ttaaactgac atccgcgcct gctgcgaacg gaaagtctgc gtcagcaacc
     4861 attgctgtta atgcaggcaa aggtggatta acagatgctg ccggtaccac gaagacacgt
     4921 ctgacttatg ctccagatgg aacattaccg gcagaagagc tggcgaccat gaatgacggt
     4981 ctttcttttg agggtgatga cggaaaggtt attcataaaa aactgaatga aaaactgacc
     5041 atcaaaggtg gtgctggtga taatgaggat gtaacagaca gtaacattcg tgttgatgca
     5101 aatgaaaagg gagatggtct tctcgtccgg atgaccagaa acctccgtga tttggccagc
     5161 gcggtttttg gtacaggtgg gcagataaca acagttaatg ataaaggcat cacaatcaca
     5221 acaccggctg cgggcggtgg tcaggggaat acagtttccc ttaccggctc cggcctgaat
     5281 aacggtggaa atcagataac cagtgtggcc agtggtctga acggcactgc gcttgctgac
     5341 gccggtggag atgttctgaa taatgcggcc aatatcggtg acctgaaaaa tgctctccgt
     5401 gacaacagtg cggcactggt gaataccggc tttggtctgc aggcggatga cggaagcaag
     5461 gtgaacacgt ctctcgggaa gcatatcacg gttggtggtg acgggcagaa tatcagcacg
     5521 actgttcaga acgggagcct gacggtcaga ctcgctgata acattcgcct ggcagatgac
     5581 ggcagtgttt ctgtcggcgg aacccggatg gataaaaacg gactgaccat caaggatggc
     5641 ccgtcagtga ctgtgaacgg tattgatgcg gctggccggg ttatttccgg cgtgaaagac
     5701 ggggtgaatg accaggacgc cgttaacgtt tcacaactga accgtgaaat cagtcaggca
     5761 acggcagcaa cgacctgggc actgaagact gaatccggtg atgaggctgt tgctgtttct
     5821 tcccagacag ttgaagtccg ccacggacag aatacccggg tttcagccat cagtaaggat
     5881 gataagggaa attacagcta tgagattgat gtgacgggga ttccggtgga gtacaccgac
     5941 agccagggga acccgcttgt caatattggt ggtcgttttt acacacagac cgaagacgct
     6001 gcgaccggga aactgacaat gacgccggct gaaccagccc gtgtcagaat aagcagtgag
     6061 cagcctcttg ttctgacaaa tgtggcggcc ggaaatgtgt caccggtatc cacggatgcg
     6121 gttaacggga gccagctggc atctgcagcc agaattgctg gcggtgataa tgttgtctgg
     6181 aaagacggaa aagcagcgct ggctcctgac accttcagtg agctgacaac tgcaggtggc
     6241 ggtaagacaa cggtgaatgg tggtgtacat aaaacaccgg ataatgtggc tgatgcagtg
     6301 aatatgctga accgtgaagg cacgaagtat tttaaagctg cctcttccgg tcaggcagca
     6361 aaagcggaag gacgtgacag cattgctgtc ggacaggggg ccgtttcccg gggacaaaac
     6421 agcattgcca tgggtaatgg tgctgaagtc acgcgtcagg ctgctgccgg ctctgttgca
     6481 gttggcagcc atgcccgtgc tggccgcgcg aataccggga cttatgccct gaacagtcag
     6541 gctgttgcgg gacgtaccgg aagtgatact gcggtggttt cttttggtca gccgggacag
     6601 gagcgtcagc tgcagtcagt ggcaccgggt gtgttgtctg cgaattcaac ggatgccgtc
     6661 aatggcagtc agttgcatgc aactaaccgt caggttgccg gaaatacaca ggcgataaac
     6721 actctcggga ataaattcag tcagctgagc taccgggtgg aagagcttaa ccgggatatc
     6781 cgtggtgttg gtgcatcggc tgcggccatg agcgggatac ctcaggccta tctgccgggt
     6841 aaaagtctga tggggctggg tgtcggtgga tatggtggtg aaagcgccat tgcaataggt
     6901 gtgtcccgaa tctccgataa cggtaagatg ataatgaaac tgaatgcagg tcagaacacc
     6961 cggggtaatt tctccgtcgg tgccggtgtg ggctggcagt ggtaacaggg aaaagcaagg
     7021 agaaatgtaa tgttatttag tcgaatgatg atgacgggtg tgctgtccct gatgcttgca
     7081 ggatgtggaa atctgagtca tgtcacggat gaaggaacga cggaagaagt ggtatggcct
     7141 tctccggaga aggtgatgtt taatcatgac ggaacacaga aaggttcgtg gccactgcca
     7201 gaaaagttac gtcagataaa ggcgggtatg aataaggacc agatttatca gctggcgggg
     7261 agaccacact ttgctgaagg gatgtctggt gtaagggaat gggattacct gttcaacttt
     7321 accggaccgg agggggatta tacctgccag tacaagatac tgtttgaccg gaacatggat
     7381 gcccgctctt tctactggaa gccggaaaac tgtctgccgg aggcagagac agcagaatac
     7441 agggagtcag tccccggtga tatattattt gattttgatt catggactct gacagagagg
     7501 gggcgtgatg cactttcccg tgtggcagag aagtttcagg cgggtcatgt tgtcggggtg
     7561 aagatagaag gatttacgga ccatcttggc gggcaggcgt ataaccggct cctgtcagag
     7621 aaacgggcgg aatcagtcag ggggtggttt attgcccggg gattcagggc tgaaaggata
     7681 catgcaacgg gccgtggata tgatgcacca gtggtgttct gcccggatgt ggagggcgaa
     7741 gcactgaagg aatgtctgcg ccccaaccgc agggttgaaa taaccggaca gagcgttcgt
     7801 taatatgcag tgtagccccg gaatgtccgg ggcttttttg tgcacagccc ccagctaggc
     7861 tctaggctgt aatgtcgatc agttagtcga acaatggcga caactacctt gaaaaatgcc
     7921 gcgaatcggt gaacgattca gatgcgatta tgtattgaag cgatgtgtcg ggtacttggt
     7981 gtatacagga tcggttatta cgactgagta cagcatgacc ctcagacaga aaacaaagtg
     8041 atgagcggct aaaactggag attaaggtgg cacatatccg cacacgcgaa acatatggaa
     8101 cctagctgct ccagacggag ctggcagata acggaattat cgtcggtcgt gacagactgg
     8161 cacgtcttcg taaggagctg aggctgcact gtaagctgaa acgcaagttc agggcgaaca
     8221 cgaactctgg tcacaatctg ccggttgcgc cgaatctcct gaatcagaac tttacgccta
     8281 cagcacctaa tcaggtcttg gttgcagata taacgtatgt tgccccccgg aaggatggct
     8341 gtatatcgct ggcgtcaaag atgtttatac gtgcgaaatt gtcggctacg ccatgggaga
     8401 gcgcatgaca aaagagctga caggtaaagc cctgtttatg gcgctcagga accagcgctc
     8461 acctaccggg ctaatccacc actccgatcg tggttcacag tactgcgcat acgattacag
     8521 ggccatacag gagcagtttg gtctgaaaat atcaatgtcg cgtaaagtta actgttacga
     8581 caatgctccg atggaaagct tctggggaac gctgaaaaat gaaagtctga gccactatcg
     8641 ttttaaaagc cgggatgagg ccatctcagt aatacgggaa tacattgaga ttttctacaa
     8701 tcgtcagcgt cgtcactctc gtctggggaa tatctccccg acagccttca ggataaaata
     8761 ttatcagatg gctgcttaga aaaagaacaa atggttatgt ccgctattgc ctgtacccct
     8821 cactccggcg aaacaccaga tgacttttcc tgccgggtaa atagattctt ctgaaaaatt
     8881 ggagactaat gtgaaatctc tggaatacat taattttgtt aacgtattgg tgataaaaaa
     8941 ctcagcatta tttattatat aaagttccaa attctatgaa gcttacaaga tattgttttt
     9001 ttgcctgtga taattgcctg aggctaagtc atacataatg ccgttggagt ttgcaggata
     9061 aaaaatatca attatgtgat atgaagtaag aagtccatcc cactctttat c
//
LOCUS       DAZGQL010000158         8786 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.158, whole genome shotgun sequence.
ACCESSION   DAZGQL010000158 DAZGQL010000000
VERSION     DAZGQL010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8786
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.158"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            53..187
                     /locus_tag="ACHKIR_004750"
     CDS             53..187
                     /locus_tag="ACHKIR_004750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992387.1"
                     /translation="MNREFEIWIRLRYGYRYDLTRDAHGFYCREVVKRMFDVWCHCRG
                     "
     gene            206..460
                     /gene="xisR"
                     /locus_tag="ACHKIR_004751"
     CDS             206..460
                     /gene="xisR"
                     /locus_tag="ACHKIR_004751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFO9992388.1"
                     /translation="MQTIIYQITPSKWCTERVLIASTGLKPGTIERARRKSWMQGKEY
                     RHYAVEGDPGHYSECLYNIEEIMRWIENQKQPGVKNASSG"
     gene            494..1780
                     /locus_tag="ACHKIR_004752"
     CDS             494..1780
                     /locus_tag="ACHKIR_004752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO9992389.1"
                     /translation="MSNTSYPTGVENHGGSLRIWFHYNGKRVRENLGVPDTAKNRKIA
                     GELRTSVCFAIRMGNFDYAAQFPESPNLKHFGLGKKEITIKALSEKWLDLKKIEIGGN
                     AFNRYQSVVRNMLPRIGESRLASSVTKEDLLFIRKDLLTGYHNLSNGKTTPVKGRSVV
                     TVNYYMTTIAGMFQFAADNGYTESNPFNGLTPLKRSRTEPDPLTHDEFIRFIDACHHQ
                     QTKNLWSLAVYTGIRHGELISLAWEDIDLKAKTMTIRRNYTKLGEFTLPKTEAGTDRV
                     IHLVQPAIETLKSQAEMTRFGTQYQIDVKLREFGRTVSHECTFVFNPQLVKKCQQAGY
                     HYKADSIRDSWASALRRAGLRHRKAYQSRHTYACWALAAGANPSFIANQMGHANAQMV
                     FNVYGAWMKDNNIGQIELLNKELAESVPYMPHRARR"
     gene            1782..2561
                     /locus_tag="ACHKIR_004753"
     CDS             1782..2561
                     /locus_tag="ACHKIR_004753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFO9992390.1"
                     /translation="MISFSIIELTHGSRHVNCVEGNTTLYALPKPEVVLRWREQTTDD
                     FRFCFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQLPATFGPRELPALW
                     HFLDSLPGEFNYGVEVRHPQFFAKGEEEQTLNRGLHQRGVNRVILDSRPVHAARPHSE
                     AIRDAQRKKPKVPVHAVLTAKNPLIRFIGSDDMTQNRELFQVWLQKLAQWHQTTTPYL
                     FLHTPDIAQAPELVHTLWEDLRKTLPEIGAVPAIPQQSSLF"
     gene            2614..3009
                     /gene="yecN"
                     /locus_tag="ACHKIR_004754"
     CDS             2614..3009
                     /gene="yecN"
                     /locus_tag="ACHKIR_004754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HFO9992391.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            3050..3793
                     /gene="cmoA"
                     /locus_tag="ACHKIR_004755"
     CDS             3050..3793
                     /gene="cmoA"
                     /locus_tag="ACHKIR_004755"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HFO9992392.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            3790..4761
                     /gene="cmoB"
                     /locus_tag="ACHKIR_004756"
     CDS             3790..4761
                     /gene="cmoB"
                     /locus_tag="ACHKIR_004756"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HFO9992393.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSAGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIADVS
                     VTTTEEQRRTEWMVTESLADFLDPHDPGKTVEGYPAPKRAVLIARKP"
     gene            complement(4926..7355)
                     /gene="torZ"
                     /locus_tag="ACHKIR_004757"
     CDS             complement(4926..7355)
                     /gene="torZ"
                     /locus_tag="ACHKIR_004757"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HFO9992394.1"
                     /translation="MTLTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHDRIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQTSWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPICSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAAWAAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGSADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNAPK
                     LTAFDQPAVQA"
     gene            complement(7380..8480)
                     /gene="torY"
                     /locus_tag="ACHKIR_004758"
     CDS             complement(7380..8480)
                     /gene="torY"
                     /locus_tag="ACHKIR_004758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HFO9992395.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFEAHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKDGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGVLTDPALADRKPLWQYAEKLDDTYCAGCHAPIASDHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
BASE COUNT         2176 a         2202 c         2103 g         2305 t
ORIGIN      
        1 gggccccagc gcaaccggat gaactggcat aaagacagca ggagaataag tcgtgaatcg
       61 cgagtttgaa atatggatac gactcagata cgggtaccga tacgatttaa ccagagatgc
      121 tcacggcttc tactgccgtg aagttgtaaa gcgaatgttt gacgtgtggt gccactgccg
      181 tggatgaaaa ttttatgagg ttggcatgca gacaatcatc tatcagataa cccccagcaa
      241 atggtgtacg gagagagtcc tcattgcatc aacagggcta aagcctggca ccattgagcg
      301 ggcaagaaga aagtcatgga tgcagggaaa agaataccgc cattacgctg tagaaggtga
      361 tccagggcat tacagtgaat gcctgtacaa catcgaagaa attatgcgat ggatcgaaaa
      421 ccagaaacaa ccaggtgtca aaaatgcaag ttccggttaa cctgttaatg ctcctggacg
      481 tctgggaggt tcaatgagta acacatcata cccgacaggc gttgaaaacc atggcggatc
      541 actccgcata tggtttcact ataacggcaa acgtgtcaga gaaaacctcg gtgttcctga
      601 cacagccaaa aaccggaaga ttgcaggcga actcaggact tcagtttgtt ttgcaatcag
      661 aatggggaat ttcgactacg ctgcgcagtt tcctgagtcc ccaaatctga aacactttgg
      721 tctgggaaaa aaggagataa ccattaaggc gctttcagaa aaatggctgg atctgaaaaa
      781 aatcgaaata ggaggtaacg cattcaatcg gtatcaatct gtggtgagaa acatgttgcc
      841 tcgtataggg gaaagccgac tggcctcatc tgtaacaaaa gaagatttac tcttcatcag
      901 gaaagattta ttgaccgggt atcacaatct ctcaaacggc aaaacaacac ctgttaaggg
      961 gagatctgta gttacagtta attactacat gacgacaatt gcaggtatgt ttcaatttgc
     1021 agctgacaac ggctacacgg aatcaaaccc gtttaacggc ctgacaccat taaaaaggtc
     1081 cagaaccgag ccagatccgc tcacgcatga cgaattcatt cgttttattg atgcttgcca
     1141 ccatcaacaa acgaaaaacc tgtggtcttt agcagtatac acaggcatcc gtcacggtga
     1201 gctaatatct ctcgcctggg aggatattga tttaaaagct aaaacaatga ctatccgtcg
     1261 taattataca aaactcgggg agtttactct accaaaaacc gaagcgggaa cagatcgtgt
     1321 tattcatctt gttcaaccag ccattgaaac cctgaaaagt caggctgaaa tgacaagatt
     1381 tggcactcaa taccagattg acgtcaaact gcgggagttc ggacgcaccg taagccatga
     1441 atgcacgttt gtctttaatc cgcaactggt gaaaaagtgc cagcaagccg gttaccacta
     1501 caaagcggac tccatcaggg attcatgggc atcagccctg agacgggccg gactacggca
     1561 caggaaagcc tatcagtcaa ggcatactta tgcctgctgg gcactggccg caggagcgaa
     1621 tccaagcttt atcgcaaacc agatggggca cgcaaacgct cagatggtat tcaatgttta
     1681 cggagcatgg atgaaagata acaatatcgg gcagatagaa ctgctcaata aggagttagc
     1741 tgaaagtgtc ccatacatgc cccacagagc aagacgctga aataatttct ttcagtatca
     1801 ttgagttaac ccatggttcc cgccatgtta actgcgtgga gggtaacacc acgctttatg
     1861 ccctgccgaa acccgaggtt gtcctgcgct ggcgtgagca gaccacagat gacttccgct
     1921 tctgttttaa gtttccggcg accatttcgc atcaggcagc attacggcat tgcgatgatt
     1981 tagtgactga atttttgacc cgcatgtcac cgttggctcc gcgcattgga caatactggc
     2041 tgcaactgcc tgccacattc ggcccacgag agctgcctgc gctttggcat tttctcgatt
     2101 ctcttcccgg tgaatttaat tatggggtgg aagtccgcca tccacagttt ttcgccaaag
     2161 gggaagagga acaaacgctt aatcgcggtt tacatcagcg cggcgttaat cgggtgattt
     2221 tagacagccg cccggttcat gcagcacgtc cacacagtga agctattcgc gacgctcaac
     2281 gaaaaaaacc taaagttccg gtacatgctg tactgacggc gaaaaatcca ctgatccgtt
     2341 ttatcggtag tgatgatatg acgcaaaacc gggagttatt tcaggtctgg ttacaaaaat
     2401 tagcacagtg gcatcagacc actacgcctt atcttttttt acatacgcca gatattgccc
     2461 aggccccgga actggtacat accctgtggg aagacttacg taaaacgctt ccagagatcg
     2521 gagcagttcc ggctattcca cagcaatctt ctcttttctg aatttgccac ctatcataga
     2581 caggtgccat cggccatttt aaagggagtt tgtatggtaa gcgcgctgta tgccgtttta
     2641 agtgcgttgt tattaatgaa gttctctttt gatgtcgttc gcctgcgaat gcagtaccgc
     2701 gttgcctatg gcgacggcgg ttttagcgaa ctgcaaagcg ctattcgcat tcatggtaac
     2761 gcggtggaat atattcctat cgcgattgtg ttgatgctgt ttatggaaat gaatggcgca
     2821 gaaacctgga tggtgcatat ttgcggcatc gttttgcttg ctggtcgtct gatgcattat
     2881 tacggttttc atcaccgtct gttccgctgg cgacgttctg gcatgagcgc gacctggtgt
     2941 gcgctgttgc tgatggtgct ggcgaatctt tggtatatgc cctgggagtt ggttttctcc
     3001 ctgcgttagc gcacaatacg ccactttctt tttcccggat ttttacgtta tgtctcaccg
     3061 cgacacgcta ttttctgccc ctatcgccag actgggcgac tggacctttg atgaacgggt
     3121 agctgaagtc ttcccggata tgatccagcg ttccgttccc ggctattcca atattatttc
     3181 catgattggt atgttagccg agcgcttcgt tcaacctggt acgcaggttt acgatctggg
     3241 ttgttctctg ggcgcggcga cgctctcggt gcgtcgcaac attcatcatg ataattgcaa
     3301 aattattgcc atcgataact cccccgcgat gattgaacgc tgccgtcgtc atattgacgc
     3361 ctataaagcc cctacgccag tagacgtcat tgaaggtgat attcgcgata tcgccattga
     3421 aaacgcatcg atggtggtgc tgaattttac cctgcaattc ctggaacctt ccgagcgcca
     3481 ggcgttactg gataaaattt atcaagggct gaacccgggc ggcgcgctgg tgctttcgga
     3541 aaaattcagt ttcgaagatg ccaaagttgg tgaactgttg ttcaacatgc accacgactt
     3601 taaacgtgcc aacggttaca gcgaactgga gatcagccag aagcgcagca tgctggaaaa
     3661 cgtgatgctg accgattccg tggaaaccca taaagcacgc ctgcataaag ctggttttga
     3721 acatagtgag ctgtggttcc agtgctttaa ctttggttca ctggtggcat taaaagcaga
     3781 ggacgctgca tgatcgactt tggtaacttt tattctctga ttgccaaaaa tcatctttca
     3841 cactggctgg aaacgctgcc cgcgcagatt gctaactggc agcgcgagca gcagcacggg
     3901 ctgtttaagc agtggtccaa cgcggtggaa tttctgcctg aaattaaacc gtatcgtctg
     3961 gatttattgc atagcgtaac cgccgaaagc gaagagccac tgagcgccgg gcaaattaaa
     4021 cgcattgaaa cgctgatgcg caacctgatg ccgtggcgca aagggccgtt ctcactgtat
     4081 ggcgtcaaca tcgataccga atggcgttcc gactggaaat gggatcgcgt tctgccccat
     4141 ctttctgatt taaccgggcg caccattctt gatgtcggct gtggcagcgg ttatcacatg
     4201 tggcgcatga ttggcgcagg cgcgcacctc gcggtgggaa tcgaccctac gcaactgttt
     4261 ctgtgccagt ttgaagcggt gcgtaaacta ctgggtaacg atcagcgcgc acatttgtta
     4321 ccgttaggta ttgaacagct tccggcattg aaagcctttg ataccgtctt ttcgatgggc
     4381 gtgctttatc atcgtcgttc accgctggag catctctggc agttaaaaga ccaactggtg
     4441 aatgaaggtg aactggtgct ggaaacgctg gttattgatg gtgatgagaa cacggtgctg
     4501 gtgccgggcg atcgttacgc gcaaatgcgt aatgtctatt tcatcccttc cgcgctggcg
     4561 ctgaaaaact ggctgaagaa gtgtggtttt gtagatatcc gcattgcgga tgtgagcgtt
     4621 accaccacag aagagcagcg acgcaccgaa tggatggtca ccgagtctct ggccgatttt
     4681 ctcgacccgc atgatccggg taaaacggtg gaaggttatc ctgcgcctaa acgcgcggtg
     4741 ctgattgcgc gcaagccgta aaggtctggt attactgccg gatgcggcgt gaacgcctta
     4801 tccggcctac aaaaccttgc taattcaata tattgcaggg actatgtagg ccttataagc
     4861 atagcgcatc aggcagtttt acgtttgcat aacctcagcg cccgtttccg ggcgcttttc
     4921 acgtcttatg cctgaacagc tggctgatca aacgccgtta acttcggtgc attgcccgta
     4981 tatttttcga tatacaccag cgcagagtta ccggcacagg catttgccag ctgagagctg
     5041 gggatatccg ccgttaacac gttcgcactg ccgtttttac acaagccatt ttccagatct
     5101 ggccatgcac cttcatgcac gcataccacg ccttttttga tcccgtcagt caccaccgcg
     5161 cctgtcagaa tctgaccacg tttgttccag acacgcacca gatcgccatt cgcaatacca
     5221 aagcgagcag catcttcggt gtgaatagtg atcggttcac gatcggcaac cgcatatttt
     5281 ttacgcagtt ccgcatagtt aagctgactg tgtaaacggt gtgccggatg tgcggtcaga
     5341 agctgcaact gcttctcgtc ggcggaacct ttccactcat caggcgcaag ccaggttggg
     5401 tgtgccgggc aatccttata gccaaatttt tccagcgttt tggagtaaat ctcaattttg
     5461 ccgcttggcg tacccagcgc atttttcacc ggatcggcgc ggaaatcacc ataacgaacg
     5521 tattgttcgt tcttctcact gcggcgcatt tcgatcagtt tattttgctg ccagaaagca
     5581 ttaaacatcg gcatagtcac gcgttgcgca cgggcacctt tctgagcggc atcatagaaa
     5641 aatttcagcc acgccatttc atctttacct tcggtataga tctcttttcc gccaggtttg
     5701 agtaattcag caagatcggc aaacacgtca aaatcgttgc gcgcttcaaa ttgcggagcg
     5761 acagcctgct tcatcggcac aatatgctgg ttgctgtaat caccggtcat cgtcaggtca
     5821 ttgcgctcaa acgatgtggt gatcggtaat acgatatctg catgtttagc agctgcggtc
     5881 cagtagcatt cagaaacgac gatcatctcc ggtttctgcc atgctttaat cagacgatta
     5941 gtatcctggt gatgggtaaa gttaccgcca cccgcccacc agatcatttt gatattcgga
     6001 tacgtttgtt ctttaccgtt atgctgatat ttgccgccag gattttccag cgcatcgacg
     6061 atacgggcaa caggaatcgc cgtcattccg ccatcatcag cggcctcgct ggcctgtccg
     6121 gcaatcgccg cagacatttc cggcagcacg ccgccaacac gcgtcgggtt gccaccgttg
     6181 gagtaatgat aagagaatcc aaaaccgccg cccggtgtac caatttgccc taacatcgcc
     6241 gccagcgtca ccagcatcca gtgtttctgt tcaccgtatt gctggcgttg gatcccccag
     6301 cctgccatca gcatcgtgcg attcgccgcc atcagttctg caagtttgac aatctgcgct
     6361 tccggtacgc cagtaatttc cgccgcccag gccgcgctct tcggcgtatt atcgctctta
     6421 cctgtcagat actcttcaaa ctgcggatac ccggtagtgt atttttcaag gaacacttta
     6481 tcgtgtttgc cttgtgtcat cagggtatgc gcaatcccta acatcagtgc cacgtcggtg
     6541 cccatattcg gtgcgatcca ggtggcatta tcgccaaaga attcgatggt ttcggagcag
     6601 ataggatcaa tggcaatcac tggtttgcca gattttttca gctgatggaa gtattccagc
     6661 ccttgctcat cggtactgct ccaggcaatt ttgagcgtgt tcagcgggtt catcccccac
     6721 agcaccacaa cctggctgtt ttccagaatc agcggccagg aggtctgctg ttcatacacc
     6781 tctacagaac cgaccacatg cggcatgatc acctgtgccg ctccggttga ataatcgccg
     6841 ctatgcccgg aataaccgcc cgccaggttc atataacgtt gcagtaaggt ttgcgcttta
     6901 tgcaacacgc cagaagaacg ccagccgtaa gatccggcaa aaatggccga tggtccgtta
     6961 gctttacgaa tacgatcgtg ttgctcatga atcagtttta atgcttgttc ccagctcacc
     7021 tgtacatagg tatcttcgcc acgacctttc gccggttgca gtggattatc gagatagctt
     7081 tttctcacca tcggatgctg aatacgcgcc gtggtgtgta cctgatccgc cgccgtagac
     7141 tgtaaggagt tcggtatggt tttcgccagc gcgcctgtcg aagaaacaat cttgccgtcc
     7201 ttcacttcta cgttcatcgc tccccaacgg cccgcggtga gaattttgcc gcccttctct
     7261 tctgcccatg caggtaacgg tgcagccgat gtcaccacca gcgcccctgc cgcaataccg
     7321 ctatgtttaa taaattcacg tcttgttaat gtcataactt cctccctgat caacgaggat
     7381 cactgtttct cggtaatatc tttggcgttg tactggaagt accgcgtcaa aatgtccagt
     7441 tcgttttcgc tcatgctggt tcgcgcaccc attcctttgg caatggacgg ccatgcattg
     7501 acggtgtaat ggtcggaggc aataggggca tggcaaccag cgcaataggt atcgtcaagt
     7561 ttttcagcgt attgccatag aggtttacgg tccgctaatg cgggatcggt aagcacaccc
     7621 tgtaaagacg cctgacgcca ttgattgccg tattcgtcag cctgccattc cccgtttacc
     7681 gtcagcgcct tgataccttc ttcacttaat gtggctagcg ccagccgttg acctgccgcc
     7741 aggtagagcg tgttttcact gccctgcatt tgataaccct gcaacagaac gatcggctgt
     7801 ttgccactgg catcaacgac ggtgagatcg gtaccaggat tcacggtagc cagttcgcct
     7861 atgcgagaag ttttgaaagg ataaatatgt gcgccattag taactgaagt agcggcctga
     7921 ctttccagct catgcgccgc gttgtcatcc atttttattt ctggcggaaa atgggcaatg
     7981 cctttatggc aatcgataca ggtttcgccg tccttttgcg ctttattatg cattttctgc
     8041 gcagattcac tttgcgaggc aatatccatg gcatcaaaag aatggcaact acggcaggtt
     8101 gcagagtcag tggcttttaa ttctttccat actgtttcgg ccatttcctg gcgatgagct
     8161 tcgaacttat catcactgtc tattttgccg ctaacaaatt catgataaat atctttagat
     8221 gcttttaatt tagcaaataa ataatccatc cctgactttg gaatatggca atcggcacat
     8281 tccgcacgta tccctttctg gtttgaaaag tggacagttc cctgatattc ctcaaaaggt
     8341 ttactcatcg agtggcagga aaggcaaaat gcggtatccg acgttttatg taaggctttt
     8401 tgcgccagta ataacccgcc gccaccaacc acaaccgcta tcagcaaaaa caataaccca
     8461 atgcgttttt tccctcgcat agattcctct aattaaattc caggacaccg ataccattgt
     8521 ataaataaat acacaacgaa ttgactactt gcttaattcg gcacaacact gcctgaaata
     8581 atcgataaag aatatgattt attacaatgt aatcattaat tgctaaggaa taacctagtt
     8641 gttatttaga atatatattt gtcagttttg gcaatttaaa tcacataatg aatgtgaata
     8701 tataaaatat ttttagcaca acgctatgcg cactgtcacc cgctcaacag atcacggcaa
     8761 tcgttcgttt tttatactgc tcagag
//
LOCUS       DAZGQL010000159         8677 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.159, whole genome shotgun sequence.
ACCESSION   DAZGQL010000159 DAZGQL010000000
VERSION     DAZGQL010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8677
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.159"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            37..831
                     /gene="hyxR"
                     /locus_tag="ACHKIR_004759"
     CDS             37..831
                     /gene="hyxR"
                     /locus_tag="ACHKIR_004759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator HyxR"
                     /protein_id="HFO9992396.1"
                     /translation="MKKPLVIISACQFTRLALESLIPTDRYIVRVYSNVTTEVEFVLK
                     TSCGYLLADIPSLPSGELVLLACLVRLRSDAGQWQVCLTGDSDWFDNAPAASLYNGFP
                     RIGTMLTASRYQNQIIRWLLRPLPGDMSDFWLLTARELSVLKILMQGMSFSEIARYEQ
                     RSSKTLHAIATRALMKLGLGTLSDFRLLYTGCGDSRVKRNIRLHAKYIGQHSAHRLTQ
                     YVQNMVSGILYPEKVAVGESINSDAISGKVLSGQRKNMSKKRPGLL"
     gene            985..1746
                     /gene="hyxA"
                     /locus_tag="ACHKIR_004760"
     CDS             985..1746
                     /gene="hyxA"
                     /locus_tag="ACHKIR_004760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein HyxA"
                     /protein_id="HFO9992397.1"
                     /translation="MRFVLFCPSGIVPAQFAALSTGSVGNVTCIRTEEELRNKLRHRP
                     QSVVISAGRPAECAEMWFRFYRDHSFVVVLCVAPFFLPPDVSISGVLKNLRLLKPGMS
                     VERVISIANASGGFSGLKHAEILPVMDSYSVFMKEVNNRTKTIVMSERFPEKQKKVLS
                     LLLAGHSWEYSAQFLKTGIRQIWLAEQSLKKRWGIPDSMSLREALLLSSNNFHGDGNA
                     LETTNAMTLRENGNTNRYSVVANAGTQALSLHKYK"
     gene            1788..4364
                     /locus_tag="ACHKIR_004761"
     CDS             1788..4364
                     /locus_tag="ACHKIR_004761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFO9992398.1"
                     /translation="MKNSKAFYRSALATAIVMALSAPAFAADKAVSTDSVTLNKDKTT
                     LDQDVVINGDNKITAVTIETSDSDKDLNVTFGGHDITAASTVNQGFVEGVKVSGDKNV
                     VINATGSTITAQGEGTYVRSAMVISSTGDVVVNGGNFVAKNEKGSATGISLEGAQGNN
                     VTLNGTTINAQGNKSSSNASTAIFAQKGSVLNGFNGDATDNITLAGSNIINGRIETIL
                     IAQENKGTHTVNLKIKDGSVIGAANNKQTIYASASAQGKGSATQNLNLSVADSTIYSD
                     VLALSESENSAATTTNVNMNVARSYWEGNAYAYAYAYAGVYADDDKASSNLNINLSDG
                     SVWKGNVAGSTTTGSTGTTENQSATVSLSDSTWKGNVGDGLDGGARVTLKNSSWTGDV
                     GNAKTAVFLTDGSVWNGAVSINAPVDRASVPMGSVQPNKSSVSLQNGSVWNVTGASTV
                     DALAVKDSTVNITKATVNTGTFASQNGTLIVDASSENTLDISGKASGDLRVYSAGSLD
                     LINEQTAFISTGKDSTLKATGTTEGGLYQYDLTQGADGNFYFVKNTHKASNASSVIQA
                     MAAAPANVANLQADTLSARQDAVRLSENDEGGVWIQYFGGKQKHTTAGNASYDLDVNG
                     VMLGGDTRFMTEDGSWLAGVAMSSAKGDMTTMQSKGDTEGYSFHAYLSRQYNNGIFID
                     TAAQFGHYSNTADVRLMNGGGTIKADFNTNGFGAMVKGGYTWKDGNGLFIQPYAKLSA
                     LTLEGVDYQLNGVDVHSDSYNSVLGEAGTRVGYDFAVGNSTVKPYLNLAALNEFSDGN
                     KVRLGDESVNASIDGAAFRVGAGVQADITKNMGAYASLDYTKGDDIENPLQGVVGINV
                     TW"
     gene            4546..5214
                     /gene="ykgH"
                     /locus_tag="ACHKIR_004762"
     CDS             4546..5214
                     /gene="ykgH"
                     /locus_tag="ACHKIR_004762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkgH"
                     /protein_id="HFO9992399.1"
                     /translation="MNEQIKQDIDLIEILFYLKKKIRVILFIIAICMAMVLLFLYINK
                     DNIKVSYSLKINQTTPGILVSCDSNNNFACQTTMTEDVIQRITTFFQTRPDVKNREIK
                     LEWSGDKRDLPTAEAEISRVQASIIKWYASEYHNGRQVLDEIQTPSAINSELYTKMIY
                     LTRNWSLYPNGDGCVTISSPEIKSKYPAAICLILGFFLSIVISVMFCLVKKMVDEYQQ
                     NSGQ"
     gene            complement(5455..6150)
                     /locus_tag="ACHKIR_004763"
     CDS             complement(5455..6150)
                     /locus_tag="ACHKIR_004763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFO9992400.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERFTQLTQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGIENISQAEQAKVGVVYAEYGLTESGGVVLFSAAGRGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(6143..7570)
                     /locus_tag="ACHKIR_004764"
     CDS             complement(6143..7570)
                     /locus_tag="ACHKIR_004764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFO9992401.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(7581..8300)
                     /locus_tag="ACHKIR_004765"
     CDS             complement(7581..8300)
                     /locus_tag="ACHKIR_004765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFO9992402.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNTLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVAHLMEVRPEYLIGADVSCLLNISG
                     RLQREGLKVKVMHIAEVLMSR"
BASE COUNT         2333 a         2053 c         2011 g         2280 t
ORIGIN      
        1 tgtcaggttt tgtagcaatg gcgtggacga gtctgaatga agaagcctct ggttattatc
       61 agtgcctgtc agttcaccag gctggcactg gagtctctca tcccaacgga caggtatatc
      121 gtgcgggtgt acagcaacgt cactacagag gttgaattcg ttttaaagac ctcctgtggc
      181 tacctgttag ctgatatccc atcgcttcct tcgggagagt tggttcttct ggcttgtctt
      241 gtccgcttgc ggagtgatgc cgggcaatgg caggtctgcc tgaccggtga ctccgactgg
      301 ttcgataatg ctcctgctgc aagtctgtat aacggcttcc cacgcatcgg caccatgctg
      361 acagcgagtc gttatcagaa tcaaattatc cgctggttac tacgtccttt gcccggcgat
      421 atgagcgatt tctggctcct gactgcacgg gagttgagcg tgcttaagat tctgatgcag
      481 ggtatgagtt tcagtgagat tgcccgttat gaacaacgca gttcaaagac cctgcatgcg
      541 attgccacac gggctttaat gaaacttgga ctgggaacac tgagcgattt caggctgctc
      601 tatacgggat gcggtgacag cagagtgaaa cggaatatac ggctacatgc aaaatatatc
      661 ggacaacatt ccgctcatcg actcactcag tatgtgcaga acatggtatc cggaattctt
      721 tatccagaga aagttgccgt tggtgaatca ataaattctg acgccatatc cggaaaagta
      781 ctttccggac aaagaaaaaa tatgtctaaa aaaagaccag gattgttatg acacagcagc
      841 gttgttacta ccccccactg cggcgcatac ggactcgcta cagtgtagga gccaaaatat
      901 ttctactgct ctgggctgca ggattgggag gcacgctgta cagcctgtac agcgtgctgg
      961 cgtactgaca tctgtgaggg aaggatgcgt ttcgttcttt tctgtccctc aggaattgta
     1021 ccagcgcagt tcgcagctct atcaaccggt tctgtcggca atgttacctg catcagaaca
     1081 gaagaggagt tacgcaataa attgcggcac aggccacaga gtgtcgtgat atcggcaggg
     1141 cgacctgccg aatgcgctga aatgtggttc cgattttatc gggatcactc attcgtggtg
     1201 gtgttgtgcg tcgcaccctt ttttctgcca ccagatgtga gtatttctgg cgttctcaaa
     1261 aacctgaggt tactgaaacc aggaatgtca gtagagcgtg tcatcagtat tgcaaatgcc
     1321 tctggtgggt tttcagggct gaaacatgca gaaattttgc cagttatgga cagttactct
     1381 gtctttatga aagaggtgaa taaccgcaca aagacaatcg tcatgtcaga acgcttcccg
     1441 gaaaagcaaa agaaggtgtt gtcgttgctt cttgccggac acagctggga atacagtgcg
     1501 cagtttctca aaacggggat aagacagata tggcttgccg agcagtcgct aaaaaagcgt
     1561 tggggaatac cggacagcat gtcattgcgg gaagccctgc tgctgagcag caataatttt
     1621 cacggtgatg gaaatgcatt ggaaacaacg aatgccatga cattgcgtga aaacggaaat
     1681 accaacaggt attccgtggt ggcaaatgca gggacgcagg ctttatctct gcataaatat
     1741 aaataagtgg gaaattgaaa ttagttacca gaatggagaa tttcttcatg aaaaacagta
     1801 aggcatttta ccgcagcgca ttagcaacag ctattgttat ggctctttct gcaccagcat
     1861 tcgctgctga taaagcggta tcaactgatt cggttacgct gaataaagat aagacaactc
     1921 tggatcaaga tgttgttatt aacggtgata acaagattac agccgtaaca attgaaacgt
     1981 cagattcaga taaagacctt aatgttactt ttggcggtca cgatattacc gccgcatcaa
     2041 cggtaaacca aggtttcgtt gaaggtgtaa aagttagcgg tgacaaaaat gttgtgatta
     2101 atgctacagg ctccaccatc acagctcaag gtgaaggcac ctatgtccgg tctgcaatgg
     2161 tcatttcttc aactggcgat gttgttgtta atggcggtaa tttcgttgca aaaaatgaaa
     2221 aaggtagtgc gacagggata tctctggaag gggcccaggg aaataatgtc acgcttaatg
     2281 gcacaaccat aaatgctcaa ggtaataaga gttccagcaa cgcctccacg gcaatttttg
     2341 ctcaaaaggg tagcgtattg aatggtttta acggtgatgc aacagacaac attacccttg
     2401 ctggctcaaa tattattaat ggtcggattg aaacaatact tattgctcag gagaataagg
     2461 gaactcatac agtcaacctg aagattaagg atggctcagt aattggggcg gctaataata
     2521 aacaaacaat ttatgcctca gcttcggcac aaggaaaagg ttcagcaacg caaaatttaa
     2581 atttgtctgt tgctgattca accatctact ctgatgtcct ggccctttct gaaagcgaga
     2641 attcagccgc taccacaaca aatgtaaata tgaacgttgc ccgctcttac tgggaaggta
     2701 atgcttatgc ttatgcttat gcttatgctg gtgtttatgc tgatgacgat aaagcgagta
     2761 gtaatctgaa tataaatctt tccgatggct cagtctggaa aggaaatgtt gcgggtagta
     2821 cgactactgg cagcaccggt actacagaaa accagtcagc aaccgtaagt ctgtctgatt
     2881 ccacctggaa aggtaacgtg ggtgacggtc tggatggtgg tgcgcgagtc acgctgaaaa
     2941 actccagctg gactggtgat gtcggcaacg ccaaaactgc tgtttttctg acggatggtt
     3001 ctgtgtggaa tggtgctgtt tccattaacg cgccagtgga ccgcgcatca gtaccaatgg
     3061 gctcggttca accaaataaa agcagtgtat ctctgcaaaa cgggtctgtc tggaatgtta
     3121 cgggagcctc aactgttgat gctctggcag taaaagacag tacggttaat atcacgaagg
     3181 ctacagtcaa tactggcacg tttgcttctc aaaacggcac tctgattgtt gatgcctctt
     3241 ctgaaaacac cctggatatc agcggtaaag cgagcggtga cttgcgtgtt tacagtgcgg
     3301 gttcattgga tcttatcaat gaacaaacgg catttatttc taccggcaaa gacagcactc
     3361 taaaagccac aggcacaacg gaaggtggtc tgtatcaata tgacctgaca cagggagctg
     3421 atggtaactt ttatttcgta aaaaacacgc ataaagcatc caacgccagc tccgtgattc
     3481 aggcaatggc agctgctccg gctaacgtcg ctaatctgca ggccgacacg ctctccgccc
     3541 gtcaggatgc tgtccgtctg agcgaaaatg acgagggtgg cgtatggatt cagtactttg
     3601 gcggtaaaca gaaacatacc accgcaggaa atgcatccta tgacctggat gtaaacggtg
     3661 taatgctggg tggtgatacc cgcttcatga ctgaagatgg tagctggctg gccggtgtgg
     3721 cgatgtcttc tgcgaaaggt gacatgacca ccatgcagag caaaggtgac actgaaggtt
     3781 acagcttcca cgcttacctg agccgccagt ataacaacgg tatcttcatt gatactgctg
     3841 cacagtttgg tcattacagc aacacggcag atgttcgcct gatgaatggt ggcggtacca
     3901 tcaaagctga ctttaacacc aatggttttg gtgcgatggt taaaggcggt tacacatgga
     3961 aagacggtaa tggcctgttt attcagccat atgccaaact gtctgctctg acgctggaag
     4021 gtgtggatta tcaactcaac ggcgtggacg ttcattctga cagctataac tctgtgctgg
     4081 gtgaggccgg tacgcgcgtg ggttatgact tcgctgtggg caactcgacc gttaaacctt
     4141 atctgaatct ggccgcactg aacgaattct ctgatggcaa caaagtccgt ctgggtgatg
     4201 agtctgtcaa tgccagcatt gacggtgcag cattccgcgt gggtgcaggt gtacaggctg
     4261 atatcaccaa aaacatggga gcatatgcaa gccttgacta caccaaaggt gacgacattg
     4321 agaatccgct acagggtgta gttggtatca atgtgacctg gtaagaataa agcagtcggt
     4381 tcccctgtcg actgttatac cccctgcagg cagcagtatg ctgcctgcag gctataaata
     4441 tccagtattc atgatgctgg atatttgctg atatttaacc cgaacattcc tgatacagac
     4501 tattaattca tttatctgcg gcagatttta ttttctgaat atattatgaa tgaacaaatc
     4561 aaacaggata tcgatctgat tgagatttta ttttatctga agaaaaagat tcgtgttatc
     4621 ctttttatta tagctatctg tatggcaatg gtgctgttgt ttctgtatat caataaagat
     4681 aatataaaag tgagttacag tctaaaaata aaccagacaa caccaggcat acttgttagc
     4741 tgtgatagca ataataattt tgcctgtcag accacaatga ctgaagatgt tattcagcga
     4801 attactacat tttttcagac cagaccagat gtcaagaaca gagaaataaa gctggaatgg
     4861 tcaggagata agagagattt accaactgct gaagcggaaa tatctcgcgt gcaggcgtct
     4921 attatcaaat ggtatgcgtc agaataccat aatggcaggc aagttcttga tgagatacaa
     4981 acgccttcag caattaacag tgagctttat acaaaaatga tatacctgac caggaactgg
     5041 tcattgtatc cgaacggtga tggctgtgta accataagct caccggaaat aaaaagtaaa
     5101 taccctgctg ccatttgtct gattctggga ttttttctaa gcattgtgat ttctgtaatg
     5161 ttttgccttg tcaaaaaaat ggtagatgaa taccaacaaa actctgggca gtaataaaca
     5221 gcattctgac agagagtgcc tgtataaaac tatagtgaca atgaccggga agggagcccg
     5281 tcttcaggaa tggattcctg tctttttcac tgtaaatatc ctggcattat taatgtcagg
     5341 atatttacag aagagcagca taaccatgac ggaacggagt taagtcaggt tgtcattagc
     5401 gctattcgca acctcgtgcc gccttcaggt ttatgtccag acttcatatc tctctcagca
     5461 atcctcaata atcagataca ccgctttcac cggaccatga accccgacga ctttgataag
     5521 ctcaatgtcc gccgttgaac tgggaccact gatgatgtta atacatgacg gcattcgttc
     5581 accggcctgc gctttctgat gcaatttttc tgcgagttgc gctacacgcg gcaggattgt
     5641 gcttttacgc aggataaaaa gagaagattc cgggagcagg ctcaatgaac gcccgcgccc
     5701 ggcggcggaa aaaagaacca cgcctcccga ttcggttaaa ccatattcag catatacgac
     5761 gcccacttta gcctgctctg cttgtgatat gttctcaata cctttcgccg gatcccaaac
     5821 aacggcattg cattcctgct gcaaacgttc gctaatcccc aattcctcca acctcgtgtc
     5881 accactaatc acgaccgact gatctcccag ctctttacac agacgtattg cagcttctgc
     5941 cgccttcgcc tcgctggtca gttcacagcg cgtcaacata acatcgctgg caaactggat
     6001 aaacgcgtca cagcgctgct gttgggtaag ttgggtaaac cgctcgttag catagttgtt
     6061 aagcggcgca tcttctgctt gcggttcaag tcgcagcggg cgacccagtg cctgagcaac
     6121 gttattcaaa aattcgctcc gattatccat tctttttctc ctgcgcctga tgtttcttaa
     6181 accaactacg gaaactctct ccgtcagctt caggaagatc gcgtgcttcc atccagtcgc
     6241 taatcgcgcc aaatttgagt ggtgttttgc cgccattgat aaaccagctt gccgcatgag
     6301 caccagccat catcccaact ttccacaatc ctggatgact attggcataa gcgaacattt
     6361 ttatcgcccg ttgctctgct tttgcggtga tccctttttc agccatcacc cgacgatgac
     6421 gcaaaatcag ttttgacagc ggaatacgca ccggacacac gctgtcacaa gcggtgcata
     6481 aagagcaggc gtagggtaaa tctttaaaat ctttatagcc gccaagtaac ggggaaatca
     6541 ccgcaccaat tggccctgga taaatagagc catatccatg accgccaata tggcgatatg
     6601 ccggacaagt attcatacaa gccccgcaac gaatacagcg cagcacatcc cgaaattcag
     6661 aggccagcac ctcagaacgc ccgttatcga caataaccag atgaaactct tcaggaccat
     6721 caacgtgccc cgcttcgcgc ggtcctgtca gccaggtgtt gtatcccgtc aaacgtgcac
     6781 caacggcact gcgcgccagc atggtgatca atacatctac ctctgcaaac gtgggagcaa
     6841 tacgctccat tcccatcact gcaatatgcg ttttaggcag cgtggtacac attcgcgcat
     6901 taccttcatt ggtcaccagg cataccgaac cggtctctgc caccgcgaaa ttacagccgg
     6961 taatacctat ttcagcactg aggaaatctt cgcggatttt ttgccgaatg aataaggtca
     7021 tcgcttcagg cgtttccggc ccctcatagc ccagacgttc gtgtagcacc cgacggatct
     7081 gatggcgatc tttatgaatt gccgggacca caacatgaga gggcggatct tgatccagct
     7141 ggagaatgta ttcacccaga tcggtttcaa tcacctgaat gccagcatcc tgcaacacat
     7201 gattgacacc aatctcttcg gtcaccatcg atttagattt caccaccttc cgggcatttt
     7261 tgcgttgggc aacctgtaaa atgtagcggg tagcgtcttc tttggttttt gcaaaataga
     7321 cgtgaccgcc gttttgcgtc actttttctg agagctggta cagataagcg tcgagattac
     7381 tcagaacatg atcacgtatc tgggcggccc gatcgcgcca ctcctcccag tgccccaatt
     7441 catcgaccat tttttgccga tttgccccga tacgctgctg cgcgtttgcc accgctttgc
     7501 gcatgatcgg atcttcaatt tgctgacgga tgcgtgtctt aaaatctgta ttactggttt
     7561 tgatcgacat ctttatatcc tcagcggctc atcaacactt cagcaatatg catcactttg
     7621 actttcagcc cttcccgttg taatcgtcca ctgatgttca gcaggcaact cacgtcagca
     7681 cctattaaat actcagggcg gacttccatc aggtgcgcaa ccttttcttt caccatctcg
     7741 ccggatattt cggccatttt gaccgagaac gtgccgccaa atccgcagca ggtatcctgt
     7801 tcagcaaagg tcaacagctc cagtccacgc acatttttca gcagcgtaag tggttcgtcc
     7861 ttcaccccca gcttacgggc caggctacaa gatgggtgat acaccgctct cccttgcaaa
     7921 ctggcaccta catcgactac ccctaatgta ttaacaataa aagaggtgag atcctgcata
     7981 cgcgcggcaa ccttttcggc acgtgatgcc cattcaggtt catccgccag atacgtcggg
     8041 taacttttta cggcataggt gcaagagcca gctggtgaaa taatgggatc gtcgttatcc
     8101 tccagtgcgg cgatcagatt tttcatccct ggaatcgctt ctttgatata accactattg
     8161 atcgcaggct gaccgcagca tccctgtttc tccgggaaat ttacgcgaca gccgagtttt
     8221 tccagtagca gcacggagtc tcgtgccatt cttgatttca gggcgtcacc aatacaggtg
     8281 acaaagaaat tgacattcac aactaatact ccattacttc atgcccattt atgcccgatg
     8341 gttcccccct tccgggggat caatcatcaa taaatatggc gttctttttt aattggctag
     8401 caacatacca cccctcattt gtatgacaaa tgataatctt tcatccaaac ttcaaatgca
     8461 ataactaatt ttatttcttt cggcatcgct cacattttat agggaaatcg acgatcattt
     8521 ttggcataag aaatccattt aaaaaggcta catcccttat acctattcag tgctacttat
     8581 tcaggggtta ttgagatata cactgatggt gtacaaacaa aatcaccaac aataattacg
     8641 tatcaagtaa tagtcggcat tttatttaat taaagca
//
LOCUS       DAZGQL010000160         8481 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.160, whole genome shotgun sequence.
ACCESSION   DAZGQL010000160 DAZGQL010000000
VERSION     DAZGQL010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8481
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.160"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..867
                     /locus_tag="ACHKIR_004766"
     CDS             <1..867
                     /locus_tag="ACHKIR_004766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFO9992403.1"
                     /translation="HLGHDNNGGIARGATPESSGSYGFVRLEGDLLRTEVAGMSLTTG
                     VYGAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYMNLTHTSSGLWADIVALGTRHSMKA
                     STDNNDFRARGWGWLGSLETALPFSITDNLMLEPQLQYTWQGLSLDDGQDNAGYVKFG
                     YGSAQHVRAGFRLGSHNDMTFGEGTSSRDTLRDSTKHGVSELPVNWWVQPSVIRTFSS
                     RGDMSMGTAAAGSNMTFSPSRNGTSLDLQAGLEARVRENITLGVQAGYAHSVSGSSAE
                     GYNGQATLNVTF"
     gene            988..3504
                     /locus_tag="ACHKIR_004767"
     CDS             988..3504
                     /locus_tag="ACHKIR_004767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992404.1"
                     /translation="MLQIVGALILLIAGFAILRLLFRALISTASALAGLILLCLFGPA
                     LLAGYITERITRLFHIRWLAGVFLTIAGMIISFMWGLDGKHIALEAHTFDSVKFILTT
                     ALAGGLLAVPLQIKNIQQNGITPEDISKEINGYYCCFYTAFFLMACSACAPLIALQYD
                     ISPSLMWWGGLLYWLAALVTLLWAASQIQALKKLTCAISQTLEEQPVLNSKSWLTSLQ
                     NDYSLPDSLTERIWLTLISQRISRGELREFELADGNWLLNNAWYERNMAGFNEQLKEN
                     LSFTPDELKTLFRNRLNLSPEANDDFLDRCLNGGDWYPFSEGRRFVSFHHVDELRICA
                     SCGLTEVHHAPENHKPDPEWYCSSLCRETETLCQEIYERPYNSFISDATANGLILMKL
                     PETWSTNEKMFASGGQGHGFAAERGNHIVDRVRLKNARILGDNNARNGADRLVSGTEI
                     QTKYCSTAARSVGAAFDGQNGQYRYMGNNGPMQLEVPRDQYAGAVETMRNKIREGKVP
                     GVTDPAEASRLIRRGHLTYTQARNITRFGTIESVTYDIAEGSVVSLAAGGISFALTAS
                     VFWLSTGDRDATLQTAAVQAGKTFTRTLAVYVTTQQLHRLSVVQGMLKHIDFSTASPT
                     VRQALQKGTGAGNISALNKVMKGTLVTSLVLVAVTTGPDMIKMLRGRISGAQFIRNLA
                     VASSGVAGGAVGSVAGGILFSPLGPFGALTGRVAGGVLGGMIASAVSGKIAGALVEED
                     RVKILAMIQEQVTWLAGSFLLTGHEIENLNANLARVIDQNALEIIFAAGIQQRAATNM
                     LIKPLVVSIIRQRPVMEYDASHLGKMVNRQEEALLPELPA"
     gene            3580..4041
                     /locus_tag="ACHKIR_004768"
     CDS             3580..4041
                     /locus_tag="ACHKIR_004768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IrmA family protein"
                     /protein_id="HFO9992405.1"
                     /translation="MIHLFKTCMITAFILGLTWSAPLRAQDQRYISIRNTDTIWLPGN
                     ICAYQFRLDNGGNDEGFGPLTITLQLKDKYGQTLVTRKMETEAFGDSNATRTTDAFLE
                     TECVENVATTEIIKATEESNGHRVSLPLSVFDPQDYHPLLITVSGKMLTDP"
     gene            4177..4266
                     /locus_tag="ACHKIR_004769"
     CDS             4177..4266
                     /locus_tag="ACHKIR_004769"
                     /inference="COORDINATES: protein motif:HMM:NF017787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HFO9992406.1"
                     /translation="MTAYRNVFIQDDPGMHFRRVIRNAEGQRR"
     gene            4444..5265
                     /locus_tag="ACHKIR_004770"
     CDS             4444..5265
                     /locus_tag="ACHKIR_004770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO9992407.1"
                     /translation="MRLVSRFGYAANQIRRDRPLTHEELMHHVPGIFGEEKHTSRSQN
                     YTYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEII
                     LLNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVQKVIEGAYEVVGVF
                     DRIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLW
                     SAYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETLLESMR"
     gene            5265..5465
                     /locus_tag="ACHKIR_004771"
     CDS             5265..5465
                     /locus_tag="ACHKIR_004771"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992408.1"
                     /translation="MKHIPEGVIQAPVSPDAPEALPGCRSISDDDLCAWCTRLLYRPG
                     ELSLCRLSTGNGIWPSVCDRNG"
     gene            5599..6072
                     /locus_tag="ACHKIR_004772"
     CDS             5599..6072
                     /locus_tag="ACHKIR_004772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO9992409.1"
                     /translation="MKTLSQNTTSSACAPETGLQQLVATIVPDEQRISFWPQHFGLIP
                     QWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMNGN
                     RAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            6087..6563
                     /gene="radC"
                     /locus_tag="ACHKIR_004773"
     CDS             6087..6563
                     /gene="radC"
                     /locus_tag="ACHKIR_004773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFO9992410.1"
                     /translation="MQQLSFLPGAMTPGELSLILRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALALVDIRVPDHLIVGGSQVFSFAEHGLL"
     gene            6632..6853
                     /locus_tag="ACHKIR_004774"
     CDS             6632..6853
                     /locus_tag="ACHKIR_004774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFO9992411.1"
                     /translation="MKIVTRGEAMRIHQQHPTSRLFPFCTGKYRWHGSAGAYTGREVQ
                     DIPGVLAVFAERHKDSFGPYVRLMSVTLN"
     gene            6929..7297
                     /gene="cbeA"
                     /locus_tag="ACHKIR_004775"
     CDS             6929..7297
                     /gene="cbeA"
                     /locus_tag="ACHKIR_004775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system cytoskeleton
                     bundling-enhancing antitoxin CbeA"
                     /protein_id="HFO9992412.1"
                     /translation="MSDTLPGTTHPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPEMKN"
     gene            7386..7763
                     /gene="cbtA"
                     /locus_tag="ACHKIR_004776"
     CDS             7386..7763
                     /gene="cbtA"
                     /locus_tag="ACHKIR_004776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000854828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system
                     cytoskeleton-binding toxin CbtA"
                     /protein_id="HFO9992413.1"
                     /translation="MKTLPVLPGQAASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPEFSACTRSQLINSIDILRARRVTG
                     LMTRDNYRTVNNITLGKYQEAKR"
     gene            7760..8248
                     /locus_tag="ACHKIR_004777"
     CDS             7760..8248
                     /locus_tag="ACHKIR_004777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFO9992414.1"
                     /translation="MKLALTLEADSVNVQALNMGRIVVDVDGVNLTELINNVAENGYS
                     LRVVEESDQQSTCTPPPFATLAGIRCSTAHITETDNAWLYSLSHQTSDVGESEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLTRRYGVSLIHLDASAGCLPGLPTF
                     DW"
     gene            8260..8457
                     /locus_tag="ACHKIR_004778"
     CDS             8260..8457
                     /locus_tag="ACHKIR_004778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFO9992415.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAREDIRTLRQLQLLHQNR"
BASE COUNT         1976 a         2305 c         2267 g         1933 t
ORIGIN      
        1 catctcggtc acgataacaa cggcggtatt gcccgtgggg ccacgccgga aagcagcggc
       61 agctatggct tcgtccgtct ggagggtgac ctgctcagaa cagaggtcgc cggtatgtca
      121 ctgacgacag gagtgtatgg tgctgcaggc cattcttccg ttgatgttaa ggatgatgac
      181 ggctcccgcg ccggcacggt ccgggatgat gccggcagcc tgggcggata tatgaacctg
      241 acacacacct cctccggcct gtgggctgac attgtggccc tgggaacccg ccacagcatg
      301 aaagcgtcaa cggacaataa cgacttccgc gcccgggggt ggggctggct gggttcactg
      361 gaaaccgctc tgcccttcag tatcactgac aatctgatgc tggagccgca actgcagtac
      421 acctggcagg gactctccct ggatgacggc caggataacg ccggttatgt gaagttcggg
      481 tatggcagtg cacaacatgt gcgtgccggt ttccgtctgg gcagccacaa cgatatgacc
      541 tttggtgaag gcacctcatc ccgtgacacc ctgcgcgaca gtacaaaaca cggtgtgagt
      601 gaactgcctg tgaactggtg ggtacaacct tctgttatcc gcaccttcag ctcccggggg
      661 gacatgagca tgggtacagc tgcagccggc agtaacatga cgttctcacc gtcccggaat
      721 ggcacgtcac tggacctgca ggccggactg gaagcccgtg tccgggaaaa tatcaccctg
      781 ggcgttcagg ccggttatgc ccacagcgtc agcggcagca gcgctgaagg gtataacggt
      841 caggccacac tgaatgtgac cttctgacag aaccatcgcc tctctgtggt cccggtcatc
      901 atgaccggga cccggaccgg cgcaacggat cttcaacgcc acattcgctg gcattaacaa
      961 taacatgata ttcatcacgg agtgactatg ttacagatag tcggcgcgct gatcctgctg
     1021 atcgcaggat ttgccattct tcgccttttg ttcagagcat taatcagcac ggcttctgcg
     1081 ctggcagggc tcatattgct gtgtctgttc ggcccggcct tactggctgg ctatatcacc
     1141 gaacgcataa cccggttgtt ccatattcgc tggctggcag gcgtatttct gacgattgcc
     1201 ggaatgatca tcagcttcat gtggggactt gatggtaaac atatcgcgct ggaggctcac
     1261 acctttgact ctgtgaaatt tattctgacc accgctctcg ccggtggtct gctggctgtt
     1321 cccctgcaga tcaaaaacat tcagcagaac gggatcacac cagaagatat cagcaaggaa
     1381 attaacgggt attactgctg tttttatact gcctttttcc ttatggcgtg ttctgcatgc
     1441 gcaccattga tcgcgttaca gtacgatatt tcaccgtcac tgatgtggtg gggcgggttg
     1501 ttgtactggc tggctgcatt agtgacgctg ctatgggcgg ccagccagat ccaggcgctg
     1561 aaaaaactga cctgtgccat cagccagaca ctggaagaac aaccggtgct caacagtaaa
     1621 tcgtggctga ccagtttgca aaacgattac agccttcctg actcactgac ggagcgcatc
     1681 tggctgacgc tcatttctca acggatttcc cggggagagc tgagggaatt tgaactggca
     1741 gacggaaact ggttactgaa caatgcctgg tatgaaagaa acatggcagg gtttaacgaa
     1801 cagttgaaag agaacctgtc attcacacct gatgaactga aaacgctctt ccggaaccgc
     1861 ctgaatttat caccggaagc gaatgacgat tttctcgatc gttgcctgaa cggcggtgac
     1921 tggtatccct tttcagaagg tcgccgtttt gtatcattcc atcacgtgga tgagcttcgt
     1981 atctgtgcct cctgcgggct gacagaagta catcatgccc cggaaaatca taagccggat
     2041 ccggaatggt actgctcctc tctttgtcgc gaaacagaaa cactgtgtca ggaaatttat
     2101 gaacgccctt acaacagctt tatttccgat gcaacggcga atggtctgat tctcatgaaa
     2161 ctgccggaaa cctggagtac aaatgagaaa atgtttgctt ccggagggca gggacatggg
     2221 tttgccgctg aacggggaaa ccatattgtc gacagagtcc gtctgaaaaa cgcacggatc
     2281 ctcggtgata ataatgccag aaatggagca gacagactgg tcagcggaac agaaatccag
     2341 acgaaatatt gttcaactgc agcccgtagc gtcggtgcgg cattcgacgg acaaaacgga
     2401 cagtatcgtt acatgggaaa taatggcccc atgcaactgg aagtcccccg tgatcagtat
     2461 gccggcgctg ttgaaaccat gaggaataag atccgcgaag gtaaagtacc cggtgtaacc
     2521 gatcccgcag aagcgtcccg gctgattcgt cggggacatc tgacttatac tcaggcccgt
     2581 aatatcaccc ggttcgggac catcgaatcg gtcacttatg atattgccga gggttcggtt
     2641 gtcagtctgg cggccggagg gatcagtttt gccctgacgg catcggtctt ctggctcagc
     2701 accggcgatc gcgatgctac cctgcagaca gctgctgtcc aggcaggaaa aaccttcacc
     2761 cgcacactgg ctgtctacgt cacaacccag caactccacc ggctcagtgt tgttcagggt
     2821 atgctgaagc atattgattt ttcgacggcc agcccgactg tccggcaggc gcttcagaag
     2881 gggaccggtg caggaaatat cagtgccctg aacaaagtga tgaagggtac gctggtgaca
     2941 tctctggtac tggtcgctgt cacaaccggc cctgacatga tcaaaatgtt gcggggacgg
     3001 atctccggtg cgcagttcat caggaatctt gccgtggcat cttccggtgt ggcaggtggt
     3061 gctgtcgggt cagtggcggg cgggatactg ttcagtccac tgggaccatt tggtgcactg
     3121 acagggcgtg tggctggcgg tgttctgggg ggaatgattg cctccgctgt atcaggaaaa
     3181 attgccggag cgctggttga agaagatcgc gtcaaaattc tggcaatgat tcaggagcag
     3241 gtgacatggc ttgccggcag tttcctgctg accggacatg agattgaaaa tctgaacgcg
     3301 aatctggccc gtgttatcga tcagaatgct ctggagatca ttttcgccgc cggtatacaa
     3361 caacgggccg cgaccaatat gttaatcaaa ccactggtgg tcagtatcat caggcaacgc
     3421 cccgtcatgg aatatgatgc atcccatctc ggcaagatgg ttaaccgaca ggaagaagca
     3481 ttactcccgg agttaccggc ataacggcta ttctgcgcct tttacgtttt atcacctgat
     3541 ttgtcagtgt catcgtcatg actcagagag gtaaatacca tgattcacct gttcaaaacc
     3601 tgcatgatta ccgccttcat tctggggtta acgtggtctg ccccactccg ggcacaggat
     3661 caacgttaca tcagtatacg caatacagat acgatatggc tcccgggaaa tatttgtgct
     3721 taccagttcc ggctggataa tggcggaaac gatgaaggat ttggccccct caccatcact
     3781 ctgcaactca aagacaaata tggtcagacg ctggtgacca gaaaaatgga aacggaagcc
     3841 tttggtgaca gtaatgccac gcgaaccaca gacgcatttc tggaaacgga gtgcgtggaa
     3901 aatgtcgcca caaccgaaat cattaaagca actgaagaaa gtaacggcca tcgtgtcagt
     3961 ctgccgttat cggttttcga tccccaggac taccatccac tgctgattac cgtttccgga
     4021 aaaatgttaa ctgatccctg aagtcagttt catgcttcag cctctgccac aacgtatggc
     4081 agaggctatt tatcaggaga aaccagatat gccgggttac acacagagta tgtcctggct
     4141 gaaggatctt tctcatacgg gcaggcggtt gctgtcataa ccgcttaccg caatgtgttt
     4201 atccaggatg acccgggaat gcatttccgt cgggttatcc gcaacgcgga aggtcagcga
     4261 cgctgacgct gccggaactc tgaagctgat gccggaaaac agcttaatgc gtggctcgcc
     4321 agtggagggc ttctcagaca ataaacgtca ttgatttcgt ccatcaggcc gcgtcttctc
     4381 cgggagacgc ggctttttca tttattgcac cagtaaatct taaccaccga taaggagcaa
     4441 agtatgcgat tagtcagtcg ttttggttat gctgcaaacc agatacgccg tgaccgtccg
     4501 ctgacacatg aagagctgat gcaccatgtg ccggggattt tcggggaaga gaaacacaca
     4561 tcccgaagcc agaactatac gtacatcccc accatcaccg tactggaaag tctgcagcgg
     4621 gaaggcttcc agcccttttt cgcctgccag acccgtgtgc gcgacccggg ccgccgggga
     4681 tacacaaaac acatgctgcg tctgcggcgg gccggagaga taaacggaga acatgtccct
     4741 gaaattattc tgctcaactc ccatgacggt acctccagct accagatgct gccgggttac
     4801 ttccggttcg tctgccagaa cgggtgcgtc tgtggtcagt ctctggggga agtgcgtgtt
     4861 ccacaccggg gaaatgtggt gcagaaagtc attgaagggg cttacgaggt ggtgggcgtt
     4921 tttgaccgga ttgaagagaa acgtgatgcc atgcagtcgc tggtcctgcc gccaccggca
     4981 cgccaggcgc tggcacaggc ggcactgact taccgttatg gtgacgaaca tcagcccgtc
     5041 accaccgccg acattctgac gccacgacgt cgggaggatt acggtaaaga cctgtggagc
     5101 gcatatcaga ccatccagga gaatatgctg aaaggcggga tttccggtcg cagtgcaaaa
     5161 ggaaaacgta tccacacccg tgccattcac agcatcgaca ccgatattaa gctcaaccgc
     5221 gcattgtggg tgatggcaga aacgctgctg gagagcatgc gctgatgaaa catatccctg
     5281 aaggcgtgat tcaggcaccc gtatcacccg acgccccgga ggcgctgccg ggatgtcgtt
     5341 ccatttctga tgatgacctg tgcgcctggt gtacccgtct gctttaccgt cccggagaac
     5401 tcagtctgtg cagactgagt acaggcaatg gcatctggcc atccgtctgt gaccggaacg
     5461 gttaacggct gtccggaatt tcagccgaat atcattcatc cctgatattg ccgggtctca
     5521 cggtcagtga tgaacgtgat gttgtcagcc cccggccttt atttcctctc atttattcac
     5581 attacaagga tttcacacat gaaaacattg tctcagaaca ccacctcttc agcctgtgca
     5641 ccggagactg gcctgcaaca actggttgcc accatagtcc ctgatgaaca gcgcatcagc
     5701 ttctggccgc agcattttgg cctcattcca cagtgggtga ccctggagcc ccgtgttttc
     5761 ggctggatgg accgtctttg cgaagactac tgtggtggta tctggaatct gtataccctg
     5821 aacaacggcg gggcatttat ggcacccgaa ccggatgacg atgatgacga aacatgggta
     5881 ctgttcaatg ccatgaacgg taaccgcgct gaaatgagcc cggaagccgc cggcattgcc
     5941 gcctgtctga tgacgtacag tcatcacgcc tgtcgtacgg agtgttatgc catgacggtc
     6001 cattattacc ggctgcggga ttacgccctg cagcatccgg aatgcagcgc cattatgcgc
     6061 atcatcgact gagcaggaga agtacaatgc aacaactttc ctttctgccc ggcgcgatga
     6121 cgcccggcga gctcagcctc attctgcggg ccctgaaaac cctggaccgc catcttcatg
     6181 aacccggcgt ggccttcacc tccacccgag cggcacggga atggctgatt ctgaacatgg
     6241 cgggactgga gcgtgaagaa ttccgggtgc tgtatctgaa caaccagaat cagctgattg
     6301 ccggtgaaac cctctttacc ggcaccatca accgcacgga agtccatccc cgggaagtga
     6361 ttaaacgcgc cctgtaccac aatgccgctg ccgtggtgct ggcacacaat cacccgtccg
     6421 gtgaagtcac acccagtaag gcagaccggc ttatcaccga acgtctggta caggcgctgg
     6481 ccctggttga catccgggtg ccggaccatc tgatagtcgg tggcagccag gttttctcct
     6541 ttgcggaaca cggtctgctt taacctgtca ccgtcacaac cacatcatac ctgttttcgc
     6601 ttttatcttc tgtcttcaga ggtatcacac tataaaaatc gtcacccgtg gtgaagccat
     6661 gcgtattcac caacaacatc cgacatcccg tctttttccg ttctgcaccg gcaagtaccg
     6721 ctggcacggc agtgccgggg cgtataccgg tcgtgaagtg caggatattc ccggtgtgct
     6781 ggccgtgttt gctgaacgcc ataaggacag ttttggcccg tatgtccggc tgatgagcgt
     6841 caccctgaac tgaatcaggg cattctgatg agcagaatta tcgccaccac cggaccattc
     6901 ttaaccgatt ttttgtgagg actttatcgt gtcagacaca ctccccggga caacgcatcc
     6961 cgacgacaat cacgaccgcc cctggtgggg gctgccctgc accgtgacgc cctgtttcgg
     7021 ggcccgactg gtacaggagg gtaaccggtt gcattacctt gctgaccgcg ccggtatcag
     7081 aggccggttc agcgacgcgg atgcgtacca tctggaccag gcctttccgc tgctgatgaa
     7141 acaactggag ctcatgctca ccagcggtga actgaatccc cgccatcagc ataccgtgac
     7201 actgtatgcg aaggggctga cctgtgaagc cgacaccctc ggctcctgtg gttacgttta
     7261 tctggctgtt tatccgacgc ccgaaatgaa aaattaactc tccagaatag ccttctgcta
     7321 cggcccggtg ttttcaccac gccacttttc catttttata tctgcatatc aggaaaatct
     7381 tcagtatgaa aacattacct gtattacccg ggcaggcggc cagttctcgc ccgtctcctg
     7441 ttgaaatctg gcagacactg ctcacccgac tgctggacca gcactatggc cttacgctga
     7501 atgacacacc gttcgctgat gaacgtgtga ttgagcagca tattgaggcc ggcatttcac
     7561 tgtgcgatgc agtgaacttt cttgttgaaa aatacgcgct ggtgcgtacc gaccagccgg
     7621 aattcagtgc ctgtacccgc tctcagttaa taaacagcat cgatatcctc cgggctcgcc
     7681 gggtgactgg cctgatgacc cgcgacaatt accgaacggt aaataacatt accctgggta
     7741 agtatcagga ggcgaaacga tgaaactggc cctgacgctg gaagctgaca gcgttaacgt
     7801 gcaggcactg aacatggggc gcattgtcgt tgacgtcgat ggtgttaatc tcactgaact
     7861 gattaataac gtcgctgaaa acggttattc actccgtgtg gtggaggaat ccgaccaaca
     7921 gtcaacctgc acacctccgc catttgcaac ccttgccggc atacgctgca gtaccgcaca
     7981 tatcacggaa acggacaacg cctggttgta ctcgctgtca caccagacca gtgacgttgg
     8041 tgaatcagaa tggattcatt tcacaggtag cggctatctg ttacgtaccg atgcgtggtc
     8101 atacccggtt ctgcggctta agcgcctggg cctgtcaaaa acgttccgcc gtctggttgt
     8161 cacacttacc cgacgttatg gcgtcagtct cattcatctg gatgccagtg ccggatgtct
     8221 gccgggttta cccactttcg actggtaacc agaaacaaca tgaaatcatt aaccacggaa
     8281 acagcgctgg atattctgat tgcatggctg caggacaata tcgactgcga atcgggaatt
     8341 atctttgaca acgatgaaga taaaacggat tcggcagcac tgttgccctg tattgaacag
     8401 gccagggagg atatccgtac cctgcgccag ctgcagcttc tgcaccagaa ccggtgaatc
     8461 tcactcacca gacttcattc c
//
LOCUS       DAZGQL010000161         7750 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.161, whole genome shotgun sequence.
ACCESSION   DAZGQL010000161 DAZGQL010000000
VERSION     DAZGQL010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7750
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.161"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(1060..2370)
                     /locus_tag="ACHKIR_004779"
     CDS             complement(1060..2370)
                     /locus_tag="ACHKIR_004779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HFO9992416.1"
                     /translation="MSELNQLQKWRREFHHIPESGWCEFITTSKIITILRGMGWQVIT
                     GNKYLSTEYIMGRNATEVADSIKRAVDSGVPHELLDEMDGFTGCVALWDTGKNGAVTA
                     FRFDIDCVQVSESKSKDHIPEKMGFASIHSGLMHACGHDGHISIGLGLAQWVSENNHK
                     LCGKIKLIFQAAEEGVRGAKPIAEGGILDDCDYFLSSHIGMGVPTGNVVASPTDLLCT
                     TKMDFFFYGTPAHAGVNPHLGNNALAAACHCATQLLAIPRHGEGMSRINIGTLNAGEG
                     RNIIPSFARMQLEVRGETETINQYMREHALKIAQGISSIFGLRFEYKVVGEATDLHND
                     PLLVNLIHYIAQEDMNMSLTEKSFGGSEDATLLIRRVQKIGGKAAYIILGSDLKAGHH
                     EVDFDFDENVLDTGVKLFSLCLERLNSPGLNKIVGNGKLESELY"
     gene            complement(2383..3654)
                     /gene="dcuC"
                     /locus_tag="ACHKIR_004780"
     CDS             complement(2383..3654)
                     /gene="dcuC"
                     /locus_tag="ACHKIR_004780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006813920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DcuC"
                     /protein_id="HFO9992417.1"
                     /translation="MFNVITGCVILIITVVLLIKRYDTRTTLIGAGILLCIVAMSPLN
                     AFSAFASRMTESGLIQAICTSMGFACVMKFTGCDTCLVHFLTKGLSRLGIFLIPAAVT
                     VSFIVCIAIPSAAGASAAVGATLIPLLISARIHPAIAGAAILSGTIGAYLNPGVAHNS
                     FISKISQTPLMEFVVFHTPTTITCGVICAVILTVVAIVRKETKCDSAGNISEQTQVEF
                     TDRDICFYLKSVAPFLPVALLMLAEFGFLGETKITVPAAMILGCLYALIVSKSNPKEL
                     VSSFFEGMGTSYANILGIIISAAVFIEGLRSVGIIDVFIRFLTDSPEFARWGGTLGPF
                     LMGLITGTGDATAFAFNEVVTPHANNFGYDTNTLGAAVMLASALGRNMSPLAGATIIC
                     AGLAGVNPVEIAKRTAPGMIVTVLFIALLFL"
     gene            3790..4707
                     /locus_tag="ACHKIR_004781"
     CDS             3790..4707
                     /locus_tag="ACHKIR_004781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005284674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO9992418.1"
                     /translation="MKYMPKLQQIKFFNEIVLCGSLRAAARKMNISQPALSRTIKELE
                     NVLGCNLLIRTRDGIVLTEAGKIFSVHSRMILEEIETAALEVAKVNIDNTGKVAFGIS
                     SLFGVTILNNVLDNFRMKYPKKRVMIREGQLSSLIPFLREGVLDFAMGTLTKDMPVGD
                     FIVVELFNAPFTIVARKHHPLRNCTSIDELATARWVFPETQMGYYHYISRLIPFGHPD
                     EPYAPIFTDSVVCIMNLVLYDDYITVLSRARLQEARFKEILCSIPVKYQLPVSQYGLI
                     YPRKRLMTAPAQIMIEYFQQEIQRYDWNG"
     gene            complement(4956..5123)
                     /locus_tag="ACHKIR_004782"
     CDS             complement(4956..5123)
                     /locus_tag="ACHKIR_004782"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992419.1"
                     /translation="MHASPVMTMVVCPTVLLCQNGHQKDQYRESLLSQMIASGHKELT
                     RVWQMNEIQAM"
     gene            5272..6105
                     /gene="iolR"
                     /locus_tag="ACHKIR_004783"
     CDS             5272..6105
                     /gene="iolR"
                     /locus_tag="ACHKIR_004783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inositol utilization transcriptional
                     regulator IolR"
                     /protein_id="HFO9992420.1"
                     /translation="MSKHQTQLSLLQDDIRSRYDSLSKRLKQVAQYILDNSNSVAFDT
                     VASIAQHAEVPPSTLIRFANAFGFSGFNEMKQVFRQHLMEETVSYTERARLFRQKNIN
                     DGEPTPEKPGEILKLFSMVNAQVLQQLPMQIKNEQLDAAVSLLSKADNIYVIGLRRSF
                     SVACYLTYALRHLERRAFLIDGIGGMFSEQLSMISSNDVVIAISYSPYAQEVVELVEL
                     GAKRDAHHIVITDSQVSPLAAFSEVCFVIREAQVDGFRSQVASMCLAQTLAVSLALAA
                     E"
     gene            complement(6158..7591)
                     /gene="iolT1"
                     /locus_tag="ACHKIR_004784"
     CDS             complement(6158..7591)
                     /gene="iolT1"
                     /locus_tag="ACHKIR_004784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000495231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inositol import MFS transporter IolT1"
                     /protein_id="HFO9992421.1"
                     /translation="MPISVNRYNTGYILRICSIAALGGILFGYDTAVISGAISSLTSY
                     FHLSPAETGWAVSSVVVGCVVGSFSSGYLSKRFGRKKSLMISALLFTISAVGTSLSYT
                     FLHFIIYRIVGGLAVGLAATVSPMYMSEVSPKDMRGRALSMQQFAIVFGQILIFYVNY
                     KIASIATNTWLIEIGWRYMFAAGIAPCILFCILVFFIPESPRWMMMAGREEETLKILT
                     KISNEEHARHLLTDIKTSLQNDRLTAHQKLNYHDSNVRFILFLGCMIAMLQQVTGVNV
                     MMYYAPIVLRDVTGSAQEALFQTIWIGVIQLVGSIIGAMIMDKMGRLSLMRKGTIGSI
                     IGLLLTSWALYSQTTGYYALFGMLFFMIFYALSWGVGAWVLISEIFPNRMRSQGMSIS
                     VGFMWIANFMVSQFFPIINENPWLLAHFHGAFPMWIFAACCVFSYFFICRYLPETKGV
                     SLEKMEDIVLTKRRNKPQPIQAERSSD"
BASE COUNT         2321 a         1613 c         1625 g         2191 t
ORIGIN      
        1 cctcttcgga ttttgatggg gcattatgga ctgggataag aaagcttatt tcattcttta
       61 aaaaatagat ctatagcgta tcgttccggc gaactcatcc tgatgcgttt tcggggggcc
      121 atcggtgaaa catacgaatg atcgccctct gacgatggat gatttatttt gttgcacaga
      181 agcgatcgct gggcaggtac cggaggttgg ctctgaagct ctaattgcgt caggttgcga
      241 agtgttgaca cgggtattgg tgagaactgc cccgggaacg actctgggct gattctggtt
      301 ctgattcgct gtcagcccgt gtggttactg gcctggctga tttcctgtcg tcaggcgttt
      361 aactcccgac tgcaggtatc acttaccaag agaaggtaaa tccttttaga cgtacagtga
      421 aagttgtctg gtcataatct gaagcatctg acaaagttgt gtccaccaaa taataagtag
      481 tgggaaccaa agtgtcatat atacagaaaa atataactca ccgatttcct tcacgttaat
      541 acgcaggggg aggtatataa cagtccgttc aggtattgga taaatattta gagtggaatg
      601 attacagtta tactgtggat gggagaagat atttactgtt aattgattat catttttaat
      661 ttgtattata ataacaaggt cccgtaaatt ccggactaca gagtccagcc agtttgctca
      721 tccgtcaatt caggtagatg cgtcaggctt tgtggctggc ggaaaatggc tcgttcgctg
      781 atattgttgc gtcatacaaa aacagtacgt gatgagtatg aggctttttt atctgtatat
      841 aatctcttat tctgtaaatt aggatagggt aatctcgcaa tcatcctctt cccatatcac
      901 cagtatatgc tgagccgtgt ttgtctattt ggttaaaatt cgctacaatc ctattctctc
      961 ttttgggaat ataaaagtct ccattttctc tttattcgtt aatggagact gtatttaaag
     1021 gcagaaatca ggagtgattg acaggatacc tgtcagcaat caatacaatt cagattccag
     1081 ttttccgttc cctacaattt tatttagtcc gggagagttc aatctctcca gacataacga
     1141 aaacagttta acgcctgtat ccagcacatt ctcatcaaaa tcaaaatcaa cttcgtgatg
     1201 accagctttt aaatcactgc caagtattat atatgctgct tttcctccta tcttctgtac
     1261 acggcgaata aggagcgttg catcttcact gcccccaaat gatttctctg tcaaagacat
     1321 gttcatatct tcttgtgcta tatagtgtat cagattgaca agtaaagggt cattatgtaa
     1381 atccgttgct tcaccaacca ctttgtactc aaaacgtaaa ccaaatatgg aagaaatacc
     1441 ttgtgctatt ttcagagcat gttcacgcat gtattgattt attgtttccg tttctccacg
     1501 aacttcaagt tgcatccttg caaaagacgg gattatgttc cttccttcac ctgcgtttag
     1561 cgtgccaata tttatccggg acataccttc accatggcgt ggaatggcaa gtaattgcgt
     1621 tgcacagtga caagcagctg ccagagcatt atttcccagg tgaggattta caccagcatg
     1681 tgccggagta ccatagaaaa agaaatccat ttttgtcgta cacaacaaat ctgtaggtga
     1741 agcgacaaca tttcctgtag gaactcccat accaatatgt gaagaaagga agtagtcgca
     1801 gtcatcaaga ataccacctt ctgcaatagg ttttgcaccg cgaacgcctt cttctgcagc
     1861 ctgaaaaatt agcttaattt tcccgcataa cttatggtta ttttcagata cccattgtgc
     1921 caatcctaaa ccaatcgata tatgaccatc atgtccacat gcatgcatta agccagagtg
     1981 tatggatgca aatcccattt tttcaggaat atgatctttc gattttgatt cacttacctg
     2041 tacacaatca atgtcaaaac gaaacgcagt gacagctcca ttttttcctg tatcccataa
     2101 agcaacacac cctgtaaagc catccatttc atccagcaat tcatggggaa cgcctgaatc
     2161 aactgcacgt tttatactat ctgcaacttc agttgcatta cggcccataa tatactctgt
     2221 tgacaggtat ttattccccg taataacctg ccatcccata ccacgcagaa tagtaattat
     2281 ctttgacgtt gtgataaatt cgcaccaccc actttccgga atatggtgaa attctctccg
     2341 ccatttttgt aactgattca gttcagacat atacgcctcc tgttaaagga ataataatgc
     2401 aataaacaat accgtcacaa tcattccggg ggcagtgcgt tttgcaattt caactggatt
     2461 aactccagcc agaccggcac agataatagt tgcaccagcc aacggagaca tgtttctccc
     2521 tagcgcagaa gccagcataa ctgcagcacc cagagtattt gtatcgtaac caaaattgtt
     2581 cgcatgtggt gtgacaactt cgttgaatgc gaatgcagtc gcatcccctg tgccggttat
     2641 aagtcccatc aggaatggcc ccaatgtacc gccccagcgg gcaaattctg gtgagtcagt
     2701 aagaaatctg ataaacacat caatgatacc aacagatctt aaaccttcaa tgaaaacagc
     2761 tgcggaaatt atgatcccaa gaatatttgc atatgatgtc cccataccct caaagaagct
     2821 ggaaactaat tctttgggat tggatttact gacaatcagg gcataaagac aaccgagaat
     2881 cattgcggct ggaacggtta ttttagtctc tccaagaaaa ccaaattcag ccagcataag
     2941 taatgctacg ggaagaaatg gtgctacaga tttcagataa aaacagatat cacggtcagt
     3001 gaactcaacc tgtgtttgtt ctgatatgtt tcctgcagaa tcacattttg tttcttttct
     3061 tacaatagca acaactgtaa gaataacagc acatattaca ccacatgtta ttgtagtcgg
     3121 agtatggaat acgacgaatt ccattaatgg tgtttgtgat attttagata taaatgaatt
     3181 atgtgcaaca ccgggattga ggtaagcacc gatagttcct gaaaggatag ctgctccggc
     3241 tattgcagga tgaatacgtg ctgaaataag taatggtatc agcgttgcac caacagcagc
     3301 tgatgctcca gcagcagatg gaatcgcgat gcaaacaata aaactgacgg taaccgcagc
     3361 aggaataaga aagataccaa gacgagataa tcctttcgtt agaaaatgaa ccaggcatgt
     3421 atcacaacca gtgaatttca taacacaggc aaagcccata ctggtacaaa tggcctgaat
     3481 gagaccggac tcagtcattc tgcttgcaaa tgccgaaaat gcgtttaaag gtgacatggc
     3541 aactatacat agcagaatac ctgcaccaat tagtgtcgtt cgtgtgtcgt atctttttat
     3601 caaaagcaca actgtgatta tcagaatcac acatccagtt atcacgttaa acatgagatc
     3661 ctccttcttc aaagaaggat aatctttact ttatgtgatt tgctcaaata ttgatcatga
     3721 acaaaaaaat caattaaccg gggatactat tatgatggag gtgataacag aaaattatca
     3781 ggcgaaaaaa tgaaatatat gcctaaactt caacaaatta aatttttcaa tgaaatagtc
     3841 ctgtgcggaa gtttaagagc tgctgcccgt aaaatgaata tatctcagcc agcattaagc
     3901 agaacgataa aagaactgga gaatgtatta ggatgtaacc ttctgattcg aacaagggat
     3961 ggtatcgttt taacagaagc tggaaagata ttctcggtcc attcacgtat gattcttgaa
     4021 gagattgaaa ctgcagctct tgaagttgcg aaagtaaata tagataacac aggaaaagta
     4081 gcttttggaa tttcttcgct gtttggcgta acaatactga ataatgtact tgataacttc
     4141 cggatgaaat atccgaaaaa gcgtgtgatg ataagagagg gacagttgtc ctcccttatc
     4201 cctttcctga gagaaggtgt tctggatttt gcaatgggta cattaacaaa agacatgcca
     4261 gttggagatt ttattgtggt tgaattgttt aacgcaccgt tcactatagt ggcaagaaaa
     4321 caccatcctt taaggaactg tacatcaatc gatgaacttg caactgcaag atgggtattc
     4381 ccggaaactc aaatgggata ctatcattac atatcccgtc ttattccctt tggacatccg
     4441 gatgaaccat atgctccaat atttaccgat tcggtagttt gtattatgaa tcttgttctc
     4501 tatgatgact atataacggt tctctccaga gcaaggttac aggaagcaag attcaaagaa
     4561 atattgtgtt caattcctgt taagtatcaa ctacccgtta gccagtacgg gcttatttat
     4621 ccacgaaaaa ggctaatgac ggcaccagca cagataatga ttgaatattt tcaacaagag
     4681 atacaacgat atgactggaa tggatgagca atacccggaa tgatggtgta caccgggata
     4741 acaatttatt cggatgtgcc aatcccacgg ttgtaatggg caatataatt gttagccgat
     4801 tcgctaatgg cagggaaaag cgcggcataa atataagccc acacacatgt aacgtactgc
     4861 agatactcgc ttggcagagt tgttacttct tgggaggcag taccagccat cagatattcc
     4921 cgttccatgc caaaacctga gttacccgga taccattaca ttgcctgtat ttcattcatc
     4981 tgccacacac gtgtcagctc tttgtgacct gacgcaatca tctgactcag cagcgattca
     5041 cggtactggt ctttttggtg accgttctga cagagcaata cagtgggaca aacgaccatc
     5101 gtcatcactg gagaggcgtg catggaaact cgtcaacaac tcaagttcgc cggaacggaa
     5161 cgttaacaat agaactttac tgtataaatt tgaatactgc taaatgtatg aatggttcat
     5221 tcatgttaat attgtgattt gcaaaaattt cattttatta gagtaaaata catgtctaaa
     5281 caccaaacac agctttcctt actgcaggac gacatccgca gtcgctatga cagtctcagc
     5341 aaacgtctta aacaagttgc tcagtatatt ctggataaca gtaatagcgt tgcttttgat
     5401 acggttgctt ctattgcgca gcacgcggag gtacctcctt cgacattaat tcgttttgcc
     5461 aatgcgtttg gttttagcgg attcaatgaa atgaaacaag tatttcgcca gcatttgatg
     5521 gaggaaaccg ttagctatac agaacgggct cgtctgttca gacaaaaaaa catcaatgat
     5581 ggggaaccca caccggaaaa accgggcgaa attctgaagc tattttccat ggtaaatgca
     5641 caggtactac aacaattacc gatgcagatt aaaaatgagc aactggatgc tgcagtaagt
     5701 ttattgtcaa aagccgataa tatttatgtg attggattac gtcgttcatt tagcgtggcc
     5761 tgttatttaa cctatgcatt gcgccatctt gagaggcgtg catttttaat tgatggtatt
     5821 ggaggcatgt tttctgagca actgagtatg atcagctcta acgatgtagt catcgccatc
     5881 agttattcgc catacgctca ggaggttgtt gaactggttg agttgggggc aaaacgtgat
     5941 gcacatcata tcgtcattac cgacagtcag gtcagtccgt tggctgcgtt cagtgaggtc
     6001 tgttttgtaa tacgtgaagc gcaggtggat ggcttccgtt cgcaggtagc gtcgatgtgt
     6061 ctggcgcaga cgttggccgt ctcactggct ctggctgctg aataagagga agatgccgat
     6121 aatctgtttt tcatgatcat cggcattttg gctaagctta atctgaagag cgttcagcct
     6181 gaataggttg tggcttgttc ctgcgttttg tcagcacgat atcctccatt ttctctagtg
     6241 agacaccttt ggtttccggc aggtagcggc aaataaagaa atagctaaag acacagcagg
     6301 ctgcgaatat ccacattgga aatgcaccgt ggaaatgggc cagtaaccag ggattttcgt
     6361 taattatagg aaagaactgt gaaaccataa agtttgctat ccacataaat cctacagaaa
     6421 tactcattcc ctgtgaacgc atgcggttgg ggaatatttc tgatatgagc acccatgctc
     6481 caacgcccca ggaaagtgca tagaaaatca taaagaacag cataccaaag agagcataat
     6541 agccggttgt ttggctgtac agagcccatg aagttaataa taaaccaatg atgctaccaa
     6601 tggtgccttt tcgcattagc gataaacgcc ccattttgtc catgatcatt gcgccaataa
     6661 ttgatccgac gagttgaatt acgccaatcc agatggtctg aaacagcgct tcctgggcgc
     6721 taccggtaac atctctcaat acaataggtg cgtaatacat cataacgtta acgccggtta
     6781 cttgctgtaa cattgcgatc atgcaaccga ggaaaaggat aaaacgtacg ttactgtcat
     6841 ggtaattgag cttttggtgg gccgtcagcc ggtcattttg cagagaggtt tttatatctg
     6901 ttagcaaatg tcgtgcatgt tcctcgttag atatctttgt aagaattttt agcgtttctt
     6961 cttcacgacc agccatcatc atccagcggg gggattccgg tataaagaat actaaaatac
     7021 agaacagaat gcatggggca atgccggcag caaacatata acgccagcca atctcaatca
     7081 gccatgtatt ggtcgcaatc gaggcgattt tatagttgac gtaaaatatg aggatctgac
     7141 caaaaacgat ggcaaactgt tgcatactca atgccctgcc acgcatgtcc ttaggtgaaa
     7201 cctccgacat atacattgga gatactgttg ctgcgaggcc gacagcaagt ccacctacga
     7261 tacgataaat aataaagtgc aggaaggtgt aggaaagaga tgttcccact gctgagattg
     7321 tgaacagcag ggcggaaatc atgagtgatt tcttgcgacc aaaacgtttg gataagtaac
     7381 ctgagctgaa tgatcccacc acacaaccta caacgacgct ggaaaccgcc cagccggttt
     7441 cggccggaga taggtgaaaa taactggtga gagaactgat tgcacctgaa ataacggcag
     7501 tatcgtagcc aaataatatc ccgcctaatg ctgcaataga acagatacgt aatatatagc
     7561 ctgtattgta acgattcact gatatgggca tagctgatta tctcctgagc agaaatcatc
     7621 tatctatttg acctataatg aaaaaattgt gtttggtcat ttccgtagct ctgcagttgt
     7681 acttcttgct ttaccatcgg taattacatt tcaatattct gattggtaat tttcactaag
     7741 atcaataatg
//
LOCUS       DAZGQL010000162         7561 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.162, whole genome shotgun sequence.
ACCESSION   DAZGQL010000162 DAZGQL010000000
VERSION     DAZGQL010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7561
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.162"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            54..1256
                     /locus_tag="ACHKIR_004785"
     CDS             54..1256
                     /locus_tag="ACHKIR_004785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFO9992422.1"
                     /translation="MILVCVILWGIYVAVRALINLNERFIDAVSSPAGIIGLFFGLLT
                     VFAILLRFFIYRRLRKKTAAFEQAVSELVQRERGFNETVNAVIARGIRQEKEQLARRR
                     EEFHTARQKASRAMQRIVDSAWKFKAKTLLAGVTINNWQSKYDQLRKEREAYAAVSEK
                     IAFLNLEDNSDWESVRQQFLDKVALLEKAQEEKEYQAELKRQIREEKERQDELDRRQR
                     EAEEEERRLAEQQKLIEEALRAAEGAHREELEKQRLELEQKIQEAHAQYERAKSMAQL
                     TKQGHVYIISNIGSFGEDVFKIGMTRRLEPMDRVKELSGASVPFDFDVHAMISCDDAP
                     ALEKTLHDSLEKYRINRINLRKEFFRVKLEKIINEVERHHGQMEYVADPAALQYLQSL
                     EYAENEAA"
     gene            1491..3236
                     /locus_tag="ACHKIR_004786"
     CDS             1491..3236
                     /locus_tag="ACHKIR_004786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFO9992423.1"
                     /translation="MTPGERRVASRLESFLNDDCLVWYDIPVGRKNRHPDFVIIDPEN
                     GLVFLEVKDWTVSTLRQVNQEQVTLETDGLLKSEINPLVQVRRYACDTVNALPANPCL
                     RQNDGQYKGRLNLAWAYGVVFTRITRQQLKTLAGNDENAVEKIFPSAQTICQDEMTQS
                     VLPEVFQQKIAGMFTTGFRTRVTPRMRDILRAHLFPEVTVKQNSQIKIMDIQQEILAR
                     NIGDGHRVIHGVAGSGKTMILLFRCLYLAETTPGKILVLCYNITLASYLRECIEARGL
                     ASRVTVSHFHSWCASMVKRHGIKVTADRKEYPDKCFSALEEAVNSGKITRTGYDAVLV
                     DEGHDFDSRWLALIARLFDNASRSLLLMYDDAQSIYRRERALNFSLASVGIQAQGRTS
                     VLPVNYRNPKRILHFAYAFSREYFEKHQNREIPLVQPQAGGEEGTEPEILRCASERDE
                     ARQVADWLEKRHALCGHWSDMAVLCPTERSAQQLQDVMTLRGVPFATCFDREGKKTYS
                     RRKDVVHLLMYQSSKGLEFPYVAVINASFVHSGAADESEVIPVLYVAFTRATRELLVT
                     CYRENSISRHLEDFA"
     gene            3302..3607
                     /locus_tag="ACHKIR_004787"
     CDS             3302..3607
                     /locus_tag="ACHKIR_004787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001604127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992424.1"
                     /translation="MNPLFNDIQMRLFYLNHSPYSWHWNVRFRPQEAVYIGSDTCHIT
                     ITCNQSGFHLTRDGQRLFTERYIRNLNELLPVLKRRWDVTPAIIRAVEYLSRVPVLH"
     gene            3662..3976
                     /locus_tag="ACHKIR_004788"
                     /pseudo
     CDS             3662..3976
                     /locus_tag="ACHKIR_004788"
                     /inference="COORDINATES: protein motif:HMM:NF018516.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ImpA family N-terminal
                     domain-containing protein"
     gene            4301..4450
                     /locus_tag="ACHKIR_004789"
                     /pseudo
     CDS             4301..4450
                     /locus_tag="ACHKIR_004789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309409.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4625..5059
                     /locus_tag="ACHKIR_004790"
                     /pseudo
     CDS             4625..5059
                     /locus_tag="ACHKIR_004790"
                     /inference="COORDINATES: protein motif:HMM:NF023902.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
     gene            5202..5291
                     /locus_tag="ACHKIR_004791"
                     /pseudo
     CDS             5202..5291
                     /locus_tag="ACHKIR_004791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5696..>7561
                     /locus_tag="ACHKIR_004792"
     CDS             5696..>7561
                     /locus_tag="ACHKIR_004792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA helicase domain-containing protein"
                     /protein_id="HFO9992425.1"
                     /translation="MAKMIPAFGPIDNNSYGEKTVYTLLKEGLSDDFTVIHSLPWLSA
                     ATRNLGMKLPPSGEIDFLVLHPVYGVLALEVKSGIYRVEGTVFVHIKTKKHVDIVKQT
                     RNNVHGLARWLGGATTLRLRIGYGFIFPDSYFDDKIINAAMVDASVKPFRGIFVDKSQ
                     LPEMAKHTIDIMQYWKHALGNNELGDERVQLIIKSVCPEFDGTPDWGIRIIYDNKLWL
                     RLTHEQIKVIELLSEYQRVVVTGWPGTGKTLIGIEFARRLVKGQKKVLFITFNNLLSE
                     YIRQQTPESLCDVFTWHKLCHQARNKLSLSPESPAGWFESGCCEDLLNALNKNKLRDY
                     DALIIDEAQALHSSWLETLSCWFENKRIIAFCDETQTFSFEKGTNYADLQRIINPQFS
                     FNLSVVLRCPRAVTDYLIAVSPASYQITSPRNNEPDTLKELVVTDPLSTLLNEILQLK
                     ESSVLPDEITVLVPTDLVKNSLQNDMRTLGVNIETVSRFRGMESSVVITLYSESMEDA
                     QLFCAYSRATTAFIAIYDSEKLAWREKNKFLHKLVNRKEVQRAINEAKHNSSSSVLMA
                     GFFREGELKLSTVKLAWSKHFSSWLVEFDERNSPEEMWIDYLYSYLPYPIIYWFRD"
BASE COUNT         2080 a         1567 c         1939 g         1975 t
ORIGIN      
        1 taaaggaaag tcatggcagg aagaaaaaag atgataatgc agcgggattt gtcctgattt
       61 tagtttgcgt tattctctgg gggatttatg tggcggtcag ggcgctgata aatcttaatg
      121 agcgctttat tgatgcagta tcgagtccgg caggtattat tggtttgttt tttggtttgc
      181 ttactgtatt tgcaatttta cttcgttttt ttatttaccg gagactcaga aagaaaacag
      241 cagcctttga gcaggccgtt tcagagctgg ttcagagaga gcggggtttt aatgaaactg
      301 tcaatgccgt gatagcccgg ggaatacgac aggaaaagga gcaactggcc agacgtcgtg
      361 aggaatttca taccgccaga cagaaggcca gccgtgcaat gcaacggatt gttgattccg
      421 cctggaaatt taaggcgaag actctgctgg ctggtgtaac gattaataac tggcaaagca
      481 aatatgacca gcttcgtaag gaaagagagg cttacgctgc tgtcagtgag aaaatagcct
      541 ttcttaacct ggaagataat tctgactggg agagtgtcag gcaacagttt ctggacaaag
      601 tggcattgct ggaaaaagcg caggaagaga aggaatatca ggcagagctt aagcgtcaga
      661 tacgtgaaga aaaagagcgt caggatgaac ttgaccggcg ccagcgggaa gcggaagagg
      721 aagaacggcg tcttgctgag cagcagaaac tgattgagga ggcgctgcgc gcagcagaag
      781 gggctcacag ggaggagtta gagaaacaac gcctggagct ggaacagaaa attcaggaag
      841 cacatgcgca gtatgaacgt gctaaatcca tggctcagct tacgaaacag gggcatgttt
      901 atatcatttc gaatattggt tcgtttggtg aggatgtctt taaaattggc atgacccgac
      961 ggctggaacc catggacagg gtcaaagagt taagtggtgc ttctgttccg tttgattttg
     1021 atgttcacgc catgatttcc tgtgatgatg cgcctgcgct ggaaaaaaca cttcatgaca
     1081 gtctggaaaa ataccgcatt aacaggatta acctgcgtaa agaatttttc cgcgtgaagc
     1141 ttgagaaaat cattaatgaa gtggagcgtc accacgggca gatggaatat gtggctgacc
     1201 cggcggcgct gcagtacctg cagagtctgg aatacgcaga aaatgaagca gcctgacagt
     1261 cagtgttctg attttacccc ggccggatat aacagaaggg atatctgcag ataccctgca
     1321 gcatggttca cagtattctg gtcatgttaa tgattctgtt ttttatttct gaataaagcg
     1381 acacccacga tataacatcc gtggatgtcg gtggtgatta ttttttgttc acagattaag
     1441 gtgcgtttat ggcgaaactg ataaatgtat cggcctccac tgaggagaga atgacgcctg
     1501 gggagaggag ggtggcttca cgactggaga gctttctgaa tgatgactgc cttgtctggt
     1561 acgatattcc ggtgggacgg aaaaaccggc atccggattt tgtcattatt gacccggaga
     1621 acggactggt atttctggag gtcaaggact ggacggtttc cacgctgcgc caggtaaatc
     1681 aggagcaggt cacgctggaa accgacggtc ttctgaaatc agaaatcaat ccgctggtac
     1741 aggttcgtcg ttatgcctgt gatacggtta atgccttacc tgcaaacccg tgcttacggc
     1801 agaatgacgg tcagtataag ggacggctga acctggcctg ggcgtatggt gtggtgttta
     1861 cccgcattac ccgtcagcaa ctgaagacac tggcagggaa tgatgagaat gcggtggaaa
     1921 aaatttttcc ttcagcacag acaatctgcc aggatgaaat gacgcagtct gttttacctg
     1981 aggtattcca gcaaaaaatt gccgggatgt ttaccacggg attcagaacc cgggtgacac
     2041 cccggatgag agatattctg cgcgcccatc tgtttcctga agtgaccgtg aaacagaact
     2101 cacaaatcaa aatcatggat attcagcagg agattctggc cagaaatatt ggtgacgggc
     2161 atcgtgtcat tcatggtgtg gccggttccg gaaaaaccat gattcttttg ttccgttgcc
     2221 tttacctggc tgaaaccaca cccggaaaga ttctggtgtt gtgctacaac attaccctgg
     2281 ccagctatct gcgggaatgc attgaagcca gagggttggc gtcacgggtt acggtatccc
     2341 attttcacag ctggtgtgcc tcaatggtaa aacgccacgg tattaaggtg acggccgaca
     2401 gaaaagaata tcctgataaa tgcttcagtg cactggaaga ggccgtgaac agcggaaaaa
     2461 tcacccgaac cggatatgat gctgtactgg tggatgaggg gcatgatttt gacagtcgct
     2521 ggctggccct gattgcccgt ctgtttgata acgcatcccg ttcactgctc ctgatgtacg
     2581 atgacgccca gtccatttac cggcgggagc gggccctgaa cttttctctg gccagtgtgg
     2641 ggattcaggc acagggacgc acttctgtcc tgccggtcaa ttatcgtaat ccgaaacgca
     2701 ttctgcactt tgcttatgca ttctcgcgcg agtattttga aaaacatcag aaccgtgaaa
     2761 taccgctcgt tcagccacag gcgggcggtg aagagggtac ggagccggaa atcctgcgtt
     2821 gtgcttctga acgtgatgaa gccagacaag ttgcagactg gctggagaag cgccatgccc
     2881 tgtgtggtca ctggagtgat atggctgtgc tgtgtccgac agagcgttca gcgcaacaat
     2941 tgcaggacgt tatgacacta cgcggggtgc cttttgccac ctgttttgac agggaaggca
     3001 agaagacata ctcgcgacgt aaggatgtgg tgcatttact gatgtaccag agcagcaagg
     3061 ggctggagtt cccgtatgtg gcggttatca atgcgtcttt cgttcactct ggtgcagcgg
     3121 atgagtctga agtgattccg gtgctttatg tggcgttcac ccgggcaacc cgtgaattac
     3181 tggtgacctg ttaccgtgaa aacagtatca gtcgtcacct tgaggatttt gcataatacc
     3241 ggattatttc tttattccgg actttcatca tactgttatt atcaggttta tttaattcgc
     3301 catgaacccg ttatttaatg atattcagat gcgtcttttt tatctgaacc actctccgta
     3361 ctcctggcac tggaatgtca gattcagacc ccaggaagct gtctatattg gcagcgatac
     3421 ctgtcatatc accattacct gtaatcagtc cggttttcat ttaaccaggg acggacaacg
     3481 gctgttcact gaacgttata tcagaaacct taatgagctg ttaccggttc tgaaacggcg
     3541 gtgggatgtg acaccggcaa tcatccgtgc cgtggaatac ctttcccggg tcccggtatt
     3601 acactgacat tcttttcgtc actgttaatc atgctttaat acagtaacac cgaatctgcc
     3661 gatgacttct gaagtgaaac tgaaaaccgg tggtgacccc aggtcattac cggattatgc
     3721 tgccctgcgt gatgaaatca gcaaactgac ccacccggca cgtccggatg tggactggcg
     3781 ttacgtggaa acgctctgcc tgcgtctgta tgaacacaat ggcgtggaat tacagacggc
     3841 atcctggtac accatcgccc ggatgcatac agcagggtta agtggactga atgaagggct
     3901 ggctctgatt gtggcgctga cccggcatca ctggtcagtg atgtggccgc tgaataccca
     3961 cgcccggctg gaaattatta ccggtctgtt caaccgtctg caaaaaacgc ttcgggccat
     4021 gcctccggac gaccgtgaca acctgcccct gctgtatcag accgaaacat tcctgaaggc
     4081 actgagtgac acacttgccc ggcacgaact taaacagggc agtaaagtgg ctttaccgga
     4141 agccatggtg aagggatata tcacccgcct ggaaaatcag cctgtgcagg gcgaagcgtc
     4201 gtctccggta acgcttccgg ctcaggcact ccggtctgat gctccggatg tgcaggaaca
     4261 tcagaccctg ccgcactccc gtctggtgta tgttgtttct gaaacaaaag cagagcccac
     4321 accctccctt cagaaatccc cgcccatgtt tctgaagccg tttttggctg gtgtctgtgc
     4381 ggcattgctg gctgtcagcg tggccattcc gggatggcag tttctgacac agccatcacc
     4441 ggcggagcag caactcaggg cgctgctgac acccccaccg gatgtgcttt cggcagacca
     4501 gatagcacag ctcagcagaa cacgctcact gttaaatcat acgtcagagt ggatacccct
     4561 ttccggtcaa cagataacgc gactgacaga attacccctg gcatacaacc tgcagcgcag
     4621 tgcgcagctg ctgcagcaac tgagggtact tttccccgat aatccccggg tacaggaaat
     4681 ggtggataac tggcagaaga gtgtaaggag ccgtgcgctc ccggaagagg cgatgacggg
     4741 ctggaacgaa ggcatgaccc gcctacagca gctggctgag cgcttgaacc gtctggatga
     4801 acagcgggga aaatacatga cagtcagtga actgaaaaca gaggtgtttg gcatcatgca
     4861 ggcttttaac cggcatatcc cggcagaaga gcagttacgt cgctataatg aagtccgcaa
     4921 ccagaatggc agtgaacaac agcaaaaact gacgcaggat gccctgacgg aactgttgaa
     4981 ccgctactgg tttctgagac atggagatgc aggtaagaag acgataaata ataaacagga
     5041 taaccagatt aatatctgac agattttatt gccattattt atccagaagc gatgatgcca
     5101 ttaatgcaaa atgacatctg aattgatcac agattggacc aatctgtgat caaatagaac
     5161 agaatacgca gatgataaag cggctttccc tcgaagattc acaacgtcat ctggcagaat
     5221 atgggtgaga gttgcaggct gatgcgtaca aagcttacga tgcgttgtat gaaactggcc
     5281 gggtgaaaga tgtcatccgt acggtgcgcg ccagactaaa caggcatcat gatggaaaag
     5341 tcgggtatca ttcacaggca tatttgtcag aatggtagcg tcagcgggtt ttgattacac
     5401 cagttttgaa gctcatactt cctatgaggc actgcagaaa acgaaagtgt cgtttaggcg
     5461 ctgggggagg ggttaaaaaa gtaacggcag aagtcaccct gaagttgagg cagtcaacta
     5521 ctgttagctg gctgctgcat gagagtgtgg agggagattg tatatattgg ttagtcagag
     5581 tctgcttcgg aataagtatg agtaattttt aaaatgggcg gaagttattt ttaattactg
     5641 ggctatgtaa caaatgcaga ttcctgtcag aaacatgtta taaggaaata gtaatatggc
     5701 caaaatgatt ccggcatttg gacccattga taataatagt tatggggaaa aaacagtcta
     5761 tacactgtta aaagaaggtc tgtccgatga tttcacagtg attcatagtc tcccgtggtt
     5821 aagtgcggcg acacgaaatt tagggatgaa attacctccg agtggagaaa ttgattttct
     5881 tgttctccat cctgtatatg gtgttttggc gttagaggta aagagtggaa tatatcgcgt
     5941 agaaggtact gtttttgtac atataaaaac aaaaaaacat gtagatatag tgaagcaaac
     6001 acgtaacaat gtccatggtt tagcccgttg gttaggggga gcaacaacgt tacggttgcg
     6061 tattggttat ggttttattt ttcctgatag ttatttcgat gataaaatta taaatgcagc
     6121 aatggtagat gcgtctgtta aaccattcag gggaatattt gttgataaaa gccaactccc
     6181 ggaaatggca aagcatacta ttgatataat gcaatactgg aaacatgcat tgggaaataa
     6241 tgagttgggg gatgaacggg ttcaactaat aataaaatct gtatgtcctg agtttgatgg
     6301 tactcctgac tggggaatcc gtattattta tgataataaa ttatggttaa ggctcacaca
     6361 tgaacaaatc aaggtcattg aacttctgtc agaatatcaa agagttgttg taaccgggtg
     6421 gccaggaaca ggaaagaccc tgattggaat tgagtttgca cggcggcttg ttaaaggcca
     6481 aaaaaaggtt ttatttatta ctttcaataa ccttctttca gaatatattc ggcaacaaac
     6541 tccggagtca ttatgcgacg tttttacctg gcacaaactt tgtcatcagg ctcgaaacaa
     6601 attatccctg tcccccgagt ctccggcagg ctggtttgaa tcaggatgct gcgaagattt
     6661 gcttaatgca ttaaataaaa ataaactaag agattatgat gctttaatca ttgatgaagc
     6721 acaggcgtta cattcttcgt ggctggagac tttatcttgt tggttcgaaa ataaaaggat
     6781 tatcgcattt tgtgatgaaa ctcaaacatt ttcctttgaa aaaggaacaa attacgctga
     6841 tcttcagcga ataataaatc cccaattttc ttttaactta tctgttgtgt taagatgtcc
     6901 tagagccgtt actgattatt taatagctgt cagccctgcc tcataccaaa ttacttcccc
     6961 taggaataac gaaccggata cgctaaaaga attagttgtt acagatccac tctccacatt
     7021 gcttaatgaa attttacaat tgaaagaaag ttctgtactg cctgatgaga ttactgtcct
     7081 tgttcctacg gatttagtga aaaattccct ccagaatgat atgagaactt tgggggtgaa
     7141 tattgaaaca gtatctcgtt ttcgaggtat ggaatcatct gtcgtgatta cattgtattc
     7201 cgaaagcatg gaggatgcac agttattttg tgcttattca agagctacaa ctgcttttat
     7261 tgcaatttat gattcggaga aattagcatg gagagaaaaa aataaatttt tgcataaact
     7321 ggttaacaga aaggaggttc agagagcaat taatgaagca aagcataatt cttcctccag
     7381 tgttcttatg gccggttttt tccgcgaagg agagttaaaa cttagtactg taaaattagc
     7441 atggtcgaag cacttttctt cgtggcttgt tgaatttgat gaaagaaatt ctccagaaga
     7501 aatgtggata gattatttgt attcctattt gccttacccg attatatatt ggttcagaga
     7561 t
//
LOCUS       DAZGQL010000163         7337 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.164, whole genome shotgun sequence.
ACCESSION   DAZGQL010000163 DAZGQL010000000
VERSION     DAZGQL010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7337
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.164"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..484
                     /gene="curA"
                     /locus_tag="ACHKIR_004793"
     CDS             <1..484
                     /gene="curA"
                     /locus_tag="ACHKIR_004793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HFO9992426.1"
                     /translation="KCRHATEVLGFDVCLDHHADDFAEQLAKACPKGIDIYYENVGGK
                     VFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKRIRLQGFIIAQD
                     YGHRIQEFQKEMGQWVKEDKIHYREEITDGLENAPQTFIGLLKGKNFGKVVIRVAVDD
                     "
     gene            682..1347
                     /gene="mcbR"
                     /locus_tag="ACHKIR_004794"
     CDS             682..1347
                     /gene="mcbR"
                     /locus_tag="ACHKIR_004794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HFO9992427.1"
                     /translation="MPGTEKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVGKRQLDEINRIRYELELMAVTLAVE
                     NLTPQDLAELEELLEKLQQAQEKGDMEQIINANRLFRLAIYHRSNMPILCEMIEQLWV
                     RMGPGLHYLYEAINPAELRERIENYRLLLAALKAKDKEGCRHCLAEIMQQNIAILYQQ
                     YNR"
     gene            complement(1383..3485)
                     /gene="pqqU"
                     /locus_tag="ACHKIR_004795"
     CDS             complement(1383..3485)
                     /gene="pqqU"
                     /locus_tag="ACHKIR_004795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor PqqU"
                     /protein_id="HFO9992428.1"
                     /translation="MKIFSVRQTVFPALLVLSPTVFAADEQTMIVSAAPQVISELDTP
                     AAVSVVDGEEMRLATPRINLSESLTGVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVITQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDDASKLSLIFNSVDIKADDPGGLTEAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSAQDDMSVMMYAGERETTQYQSIPMSPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNRGVPEYGQKGELRRDERN
                     LMWNVDPYLQTQWQLTDKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNVYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVKA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PAPGRNYGVGMNIAWRFE"
     gene            3727..4788
                     /locus_tag="ACHKIR_004796"
     CDS             3727..4788
                     /locus_tag="ACHKIR_004796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HFO9992429.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGATINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            complement(4901..6400)
                     /gene="ansP"
                     /locus_tag="ACHKIR_004797"
     CDS             complement(4901..6400)
                     /gene="ansP"
                     /locus_tag="ACHKIR_004797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HFO9992430.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKEGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            6667..7284
                     /locus_tag="ACHKIR_004798"
     CDS             6667..7284
                     /locus_tag="ACHKIR_004798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HFO9992431.1"
                     /translation="MIKVHGVPGWGTTISEMMLTLADIPYQFVDVSGFDHEGTSRELL
                     KTLNPLCQIPTLALENDEIMTETAAIALMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWAPDAPEQLKNNVIEYRKSLYIWLNSQLTAEPYAFGEQLTLVDC
                     YLCTMRTWGPGHEWFHDNAQNISSIAEAVCQLPKLQEVLKRNEII"
BASE COUNT         1985 a         1807 c         1781 g         1764 t
ORIGIN      
        1 aaaatgccgc catgctactg aggtgctggg cttcgatgtt tgtcttgacc atcacgcgga
       61 tgattttgcc gaacaactgg cgaaagcgtg cccaaaaggt attgatatct attatgaaaa
      121 cgtgggcggt aaggtatttg atgcagtgct acctttactt aatacatctg cgcgcattcc
      181 cgtttgtggc ttagtgagca gctataacgc tacagagcta ccacccggtc cggatcgttt
      241 acctctgctg atggctacgg tgctgaaaaa acgtattcgc ttgcaaggat ttattatcgc
      301 tcaggattat ggtcaccgca tccaagagtt tcagaaggag atggggcaat gggtgaaaga
      361 ggataaaatc cactaccgcg aagaaattac tgacggttta gagaatgcgc cacagacgtt
      421 tatcggcctg ctgaagggta aaaacttcgg caaagtggtg atccgcgtgg cggttgatga
      481 ttaaagtttc aacggcggcg taagccgcca taaaaatcca ggcaaaaagc ttattttcag
      541 ctttaattaa ctaaccacgt cattgttctg ctgctaataa caacaaaggt gagtggttta
      601 tgattattca ctttaataca ccaggtgaac tccttctgcc atgcaggcag ggttggacag
      661 aaaacacgcg aaggaattaa aatgcctgga acggaaaaaa tgaaacatgt cagtttgact
      721 ctgcaggttg agaacgacct gaaacatcag cttagtattg gcgcattaaa acctggcgca
      781 cgcctgatta ctaaaaatct ggcggagcaa ttaggtatga gtattacacc tgtgcgtgaa
      841 gcattattac gtctggtttc ggtgaatgcg ctttctgtcg caccagcgca agcatttaca
      901 gttccggaag tggggaaacg tcaattagac gaaatcaatc ggatccgcta cgagctggaa
      961 ttaatggcag ttactctggc tgttgaaaac ctcaccccgc aagacctggc ggaactcgag
     1021 gaattgctcg agaaattaca gcaggcgcaa gaaaagggcg acatggaaca aatcattaat
     1081 gcgaacaggc tatttcgctt agcgatttat catcgttcaa atatgcccat cctgtgtgag
     1141 atgattgagc aactgtgggt caggatggga cctggtttac attatctcta tgaagcgatt
     1201 aatccagcgg aattacggga gcgtatagaa aactatcgtc tattactcgc cgcgttaaaa
     1261 gcaaaagaca aagagggatg cagacattgt cttgctgaaa ttatgcaaca aaatattgct
     1321 attttatacc agcaatacaa tcgttaaatt aaatgcccgg caaataccgg gcatcaaggt
     1381 aattactcaa atcgccacgc aatattcatg ccgacaccat aatttcgccc gggcgcaggt
     1441 tcgtaatatc gcccgtttga ctcattgaca atgacagagc caacgtattc tttatcgaat
     1501 aaattatcga cacgaccaaa taaatccaca gttaaattgt ggtaattgta tttatacccg
     1561 gtgaataagc cgacgagagt ataagacggc gctttggccg tattttcatc atctgccata
     1621 atatcgccca tataacgcgc ttccgtacct gcataccagc catcttccgg tacataacct
     1681 atcgacgcaa agcccatatt acgggcgatc cctggcatcc ggttaccgtt acaatcctgt
     1741 tcattgcaaa cattgctgcg ataggtcgca tcaagccagg tccaggacgc ttttacgcga
     1801 aaatctcccg cgaaacgttg atcccatgcc agttcagcgc cttgacgacg ggtctttccg
     1861 gcatttttgt aagtcgtacg cccaccgcta ctgctatcga caacaatttc atcatcggtg
     1921 tcggtctgga acaatgcgag actaagcagc ccatcaccaa tacgcgtttt actgccgatc
     1981 tcaattgtat cgttggtgga gggttttaaa ccaaagttca taccgctttg cccatcagcg
     2041 cgataagaca gctcattaat cgtcggcgtt tcaaaaccgc gcccggctgc cagatagaca
     2101 ttccaggcat cggtcattgc atattttaac gatccggctg gtagccattt gtgataactg
     2161 gcatcaccgc tgtcgtcgcc gttacccgga gtaacgtaat gatcattgga atcaaaccac
     2221 acagagctgt agcgcacgcc agcatccagc gaaagtttgt cggtcagttg ccactgggtt
     2281 tgtaaatagg gatcgacgtt ccacatcaaa ttgcgttcgt cgcggcgcaa ctcacctttt
     2341 tgcccgtatt ccggcacgcc gcgattcagg cggaagttat tgtaaccctt gcggttttca
     2401 ctcatgtttt cgtagttcag gccggttgtg aacgtgactg gaacgcccaa ttcaccacgg
     2461 tgtgtccagc ggctgtctat cccctggtaa tggcgttgca gggtaatcac gccgcccgca
     2521 tgtgacgggt taagttgcgg ggacatgggg attgactggt actgggtcgt ttctcgctct
     2581 ccggcataca tcattacact catatcatct tgcgcgctca ggctacgttc atagcgcaac
     2641 ccagcctgag tttgcttgat ggtttttcgc gtgtcgtact gttctgcacg gggcgcttgt
     2701 tgtggattcg ccttccattc tgcttcggtt agcccacctg ggtcatctgc tttgatatcc
     2761 acactattga aaatcaggct taatttgctg gcgtcatcaa tgcgaacgcc cagtttggca
     2821 ttggctaaat ttttctgtgc gccactatga tcacgatagc cgtgggtcgt aaagcgcgtg
     2881 gttgagacgg tgtaatcgac atcgcctggc tgtgtgccgt ctcccgttgc gcccgttgct
     2941 ttcagcccat agcgccagct gccaaaactg ccgtagtaac tactggcttc aatggttggt
     3001 ggctgttgtc cggtctgggt gatgacattc attacaccgc cagacgcgtt gccatacaga
     3061 gcagagaagg ggccacgcag cacttccaca ttttgcacac tgcttaaatc gatattggat
     3121 gtctgccctt gtccgtcggg catggtagcg ggaataccgt caacatacag gcgaataccg
     3181 cgaattccgt aagtggagcg ggagccaaat ccgcgaatcg acagctgtaa atcttgcgca
     3241 tagttctgcc ggttttgtac ctgcaaacca ggcacgccag tcagtgattc agacaagtta
     3301 atgcgcggtg ttgccaggcg catctcctcg ccatccacca cgctcacggc tgctggggta
     3361 tccagttctg aaatcacctg cggcgcggca ctgacaatca tggtctgttc atcagcggca
     3421 aaaacggtgg gggaaaggac aagcagcgcg ggaaaaacgg tctgtcggac ggaaaaaatc
     3481 ttcatgaaaa aagccaggtt aagaatggga aaacaccgtc atggtaataa aattgtaaat
     3541 ttatggaaaa cgaaacggca cgatatgtta agtaattgag aaaattgtag tcgtaacggc
     3601 aagaaatgct ccacatttga gaaaataatg attaccattc ccatttataa caagagcgta
     3661 acgatgatta cgcttagcgg agcattgtga agcagcaaaa atatcggttc atcaaaggga
     3721 gtcgtcatgc atttacgtca tctgttttca tcgcgcctgc gtggttcatt actgttaggt
     3781 tcattgcttg ttgcttcatc attcagtacg caggccgcag aagaaatgct gcgtaaagcg
     3841 gtaggtaaag gtgcctacga aatggcttat agccagcaag aaaacgcgct gtggctcgcc
     3901 acttcgcaaa gccgcaaact ggataaaggc ggcgtggttt atcgtcttga tccggttact
     3961 ctggaagtga cgcaggcgat ccataacgat ctcaagccgt ttggtgctac catcaataac
     4021 acgactcaga cgttgtggtt tggtaacact gtaaacagcg cggtcacggc gatagatgcc
     4081 aaaacgggcg aagtgaaagg ccgtctggtg ctggatgatc gtaagcgcac tgaagaggtg
     4141 cgcccgctgc aaccgcgtga gctggtggca gacgatgcca cgaacaccgt ttacatcagt
     4201 ggtattggta aagagagcgt gatttgggtc gttgatggcg agaatatcaa actgaaaacc
     4261 gccatccaga acaccggtaa aatgagtacc ggtttagcgc tggatagcaa aggcaaacgt
     4321 ctttacacca ctaacgctga cggcgaattg attaccatcg acaccgccga caataaaatc
     4381 ctcagccgta aaaagctgct ggatgacggc aaagagcact tctttatcaa catcagcctt
     4441 gataccgcaa ggcagcgtgc atttatcacc gactctaaag cggcagaagt gttagtggtc
     4501 gatacccgta atggcaatat tctggcgaag gttgcggcac cggaatcact ggctgtgctg
     4561 tttaaccccg cgcgtaatga agcctacgtg acgcatcgtc aggcaggtaa agtcagtgtg
     4621 attgacgcga aaagctataa agtggtgaaa acgttcgata cgccgactca tccgaacagc
     4681 ctggcactgt ctgccgatgg caaaacgctg tatgtcagtg tgaaacaaaa atccactaaa
     4741 cagcaggaag ctacccagcc ggacgatgtg attcgtattg cgctgtaata gatatggcga
     4801 gggtgaaaat ggagagactt gcctgacttt tatcacgccg tatccggcac cttcgtcagt
     4861 aatttgaatt tgcccggcac gtagccgggc aattttgcaa ttagctcgcc gtttcaggct
     4921 taaacacaat ttcctgtttt tcttcatctt cctcgacgac tggcgcagtg ctgtgaattt
     4981 cagcaacgcg tttgcgcaca ccaaaccagc ctataaccag cagaataccg ataatcggca
     5041 gcgccgcgat agtataagtc ccgttcgggt aatcgaacgc catcagcaca aggacgctca
     5101 gcaaaaacag taatgtcagc caggaagtga agggcgcgcc aggcagttta aaactgacat
     5161 ccgctgcttt gccttcttta atcgctttac gcaggcgcat ctggcacacg atgataaacg
     5221 cccatgaagc gatgattccc agcgacgcga agttcaacac aatctcaaat acgcgcgacg
     5281 gcaccagata gttgaggaat acgccgacga catacacaac cagtgtcgcc agaatcccgg
     5341 catacggcac atgctgacga ctcattttcg ccataaaact cggtgcggaa ccgcccatcg
     5401 ccatcgagcg cagaatacgt ccggtgcagt acagacctga gttcaggctg gagagggcag
     5461 cggtcagcac cacaatgttc ataatgctgc cgatgtacgg cacacccagt ttagagaaaa
     5521 acgtcacgaa cggactttgc cccgcctgat acgcgctcca cggcaataac ataaccagca
     5581 acaccacgga gccgacgtaa aacaggccaa tacgccaaat cacactgtta atcgctttag
     5641 gcaccatggt ctgcggatct ttacattctc cggcagctgt acccaccatt tcaatggagg
     5701 caaaagcaaa cactacgccc tgaatcaaca ccagcgcagg tagcaaaccg tgggggaaga
     5761 agccgccgtt atcggtaatt aaatgaaagc cagtggtgtt gccatccagc ggctgaccac
     5821 taccgaggaa cactgtaccc acgaccaaaa aggtcacaat ggcgagcact ttaataagcg
     5881 caaaccagaa ctccatctcg gcaaaccatt tcacaccaat catattcatg gtgccaacga
     5941 tggtcagcgc agcgagcgca aagacccact gcggtacacc accaaatgcg ccccagtaat
     6001 gcatatacag tgcaacggcg gtaatatcga caatccccgt catcgcccag ttgatgaagt
     6061 acatccagcc tgcaacataa gcggctttct cacccaaaaa ctcacgggca taagagacaa
     6121 aactgccact ggaagggcgg tgtagcacca gctcacccaa tgcacgcaga ataaaaaacg
     6181 aaaacaagcc gcagattaaa tagaccagtg ccagtgctgg ccccgccatt tgcagtcggg
     6241 ctcccgcgcc taaaaacaaa cccgtaccaa tcgcgccgcc aatggcgatc atctgcacct
     6301 ggcgattgcc catcgctttg tgatacccct cttcgtgggc attaagccag cggcgtttcg
     6361 cggcgtgctg atctgaagtg tcggtgtcgt gtttactcat tgctctccct gattgcttta
     6421 atgaaaaagt catataagtt gccatgaaca atgtttattc atcctggcga tgatagttat
     6481 caccattatt ctttatgggt ataaaactat cggcgcagca tcctacccgt tatcgataaa
     6541 cgatgcaaaa catcccctta caattctgaa ggggattcat acaactgacg aaaaaatgac
     6601 aaatactttt gctggttaac ctgtgtgctg tcctagactt aatctttaaa agattgtgag
     6661 gggcatatga ttaaggtgca tggcgtaccc ggatggggca ctacaatcag tgagatgatg
     6721 ctaacactgg ctgatattcc ttatcagttc gttgatgtca gtggttttga ccacgaggga
     6781 acttcacgcg agttattgaa aaccctgaac ccgttgtgtc agattccgac actggcgctg
     6841 gaaaatgacg agattatgac ggagacagcg gcgattgcat tgatggttct cgatcgtcgc
     6901 ccggaccttg cgccaccggt tgggcgcgcc gaacgtcagc agtttcaacg gctattggtc
     6961 tggctggttg ctaatgtcta tccaacattc actttcgccg attaccctga gcgttgggct
     7021 cctgacgcac ccgaacagtt aaagaataat gttattgaat atcgtaaatc gctttatatc
     7081 tggctgaatt cacaattgac cgctgaacct tatgcgtttg gtgagcaact aacgctggtg
     7141 gactgttatc tttgcaccat gcgcacatgg gggccggggc atgaatggtt tcacgataat
     7201 gcgcagaata tcagctcgat cgctgaagct gtatgtcagt taccgaaatt acaagaagta
     7261 ttaaaaagga atgaaattat ttgaatggga taccagaaga ttatgttatt tattattcag
     7321 taactttatc cacaatg
//
LOCUS       DAZGQL010000164         7045 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.165, whole genome shotgun sequence.
ACCESSION   DAZGQL010000164 DAZGQL010000000
VERSION     DAZGQL010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7045
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.165"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            546..1535
                     /gene="araF"
                     /locus_tag="ACHKIR_004799"
     CDS             546..1535
                     /gene="araF"
                     /locus_tag="ACHKIR_004799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HFO9992432.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVEPPKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            1605..3119
                     /gene="araG"
                     /locus_tag="ACHKIR_004800"
     CDS             1605..3119
                     /gene="araG"
                     /locus_tag="ACHKIR_004800"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HFO9992433.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGMFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRTHVLGGCVINNGWEENNADHHIRSLNIKTPG
                     ADQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            3134..4120
                     /gene="araH"
                     /locus_tag="ACHKIR_004801"
     CDS             3134..4120
                     /gene="araH"
                     /locus_tag="ACHKIR_004801"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HFO9992434.1"
                     /translation="MSSVSTSGSGAPKSSFSLGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGVLCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            4287..5087
                     /gene="otsB"
                     /locus_tag="ACHKIR_004802"
     CDS             4287..5087
                     /gene="otsB"
                     /locus_tag="ACHKIR_004802"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HFO9992435.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDTILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTAIAQFPGAELETKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            5062..6486
                     /gene="otsA"
                     /locus_tag="ACHKIR_004803"
     CDS             5062..6486
                     /gene="otsA"
                     /locus_tag="ACHKIR_004803"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HFO9992436.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNKFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTACGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(6493..6921)
                     /gene="uspC"
                     /locus_tag="ACHKIR_004804"
     CDS             complement(6493..6921)
                     /gene="uspC"
                     /locus_tag="ACHKIR_004804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HFO9992437.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCRKYH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
BASE COUNT         1770 a         1651 c         1831 g         1793 t
ORIGIN      
        1 tcttgttgat attaatcgat taatttgaat tcatgatgaa gtgatggttt tataaaacgt
       61 tttttcattt ttgcgagatg gctctcatta tacgtgttct gttaattaat gagcacagtg
      121 ataattaatg gcgaaatgaa tgacgcacat tttccacatc tttgagttgt ggttattaca
      181 ccattgcaaa aactcaacgc caggtaatgc ggcctattga ctggttaaaa agaagacatc
      241 ccgcatgggt accaaagaca acaaggactt ccaggctaat cttatggatt attctgctgt
      301 gcactcgata atttgtctga caaattcgct tttccttatg tcttttcccg ctaaatttat
      361 gcacgttctc actgtaattc tgcgatgtga tattgctctc ctatggagaa ttaatttctc
      421 gctaaaacta tgtcaacaca gtcacttatc ttttagttaa aaggtaatgc tttgttttcc
      481 gattaattta acgaatgtca ttcgtttttg ccctacacaa aacgacacta aagctggaga
      541 gaaccatgca caaatttact aaagccctgg cagccattgg tctggcagcc gttatgtcac
      601 aatccgctat ggcggagaac ctgaagctcg gtttcctggt gaagcaaccg gaagagccgt
      661 ggttccagac cgaatggaag tttgccgata aagccgggaa agatttaggg tttgaagtta
      721 ttaagattgc cgtgccggat ggcgaaaaaa cattgaacgc tatcgacagc ctggctgcca
      781 gtggcgcgaa aggtttcgtt atttgtactc cggacccgaa actcggctct gccatcgtgg
      841 cgaaagcgcg tggctacgat atgaaagtca ttgccgtgga tgaccagttt gttaacgcca
      901 aaggtaagcc aatggatacc gttccgctgg tgatgatggc tgcgaccaaa attggcgaac
      961 gtcagggcca ggaactgtat aaagagatgc agaaacgtgg ctgggatgtc aaagaaagcg
     1021 cggtgatggc gattaccgcc aacgaactgg ataccgcccg ccgccgtaca acgggttcta
     1081 tggacgcact gaaagctgca ggattcccgg aaaaacaaat ttatcaggta cctaccaaat
     1141 ctaacgacat cccgggggca tttgacgctg ccaactcaat gctggttcaa catccggaag
     1201 ttaaacattg gctgatcgtc ggtatgaacg acagcaccgt gctgggcggc gtacgcgcga
     1261 cggaaggtca gggctttaaa gcggccgata tcatcggtat cggtattaac ggtgtggatg
     1321 cggtgagcga actgtctaag gcacaggcaa ccggcttcta cggttccctg ctgccaagcc
     1381 cggacgtaca tggttataag tccagcgaaa tgctttacaa ctgggtagca aaagacgttg
     1441 aaccgccaaa atttaccgaa gttaccgacg tggtgctgat cacgcgtgac aactttaaag
     1501 aagaactgga gaaaaaaggt ttaggcggta agtaatttgc cggaaagatt cccctctgca
     1561 tgatgcagag ggggtgtgaa cgaccagtga ttcacggaga cgttatgcaa cagtctaccc
     1621 cgtatctctc atttcgcggc atcggtaaaa cgtttcccgg cgttaaggcg ctgacggata
     1681 ttagttttga ctgctatgcc ggtcaggttc atgcgttgat gggtgaaaat ggcgcaggaa
     1741 aatcaactct cttaaaaatc ctcagcggaa actatgcgcc aaccacgggt tctgtagtga
     1801 ttaatgggca ggaaatgtcc ttttccgaca cgaccgcagc acttaacgcg ggggtggcga
     1861 ttatttacca ggagctgcat ctcgtgccgg aaatgaccgt cgcggaaaac atctatctcg
     1921 gccagctgcc gcataaaggc ggcattgtca atcgctcatt gctgaattat gaggcgggtt
     1981 tacaacttaa acatcttggt atggatattg acccggacac gccgctgaaa tatctctcca
     2041 ttggtcagtg gcagatggtt gaaattgcca aagccctggc gcgtaatgcc aaaattatcg
     2101 cctttgatga gccaaccagc tccctctctg cccgtgaaat cgacaatctt ttccgcgtta
     2161 ttcgtgaact gcgaaaagag gggcgggtga tcttatacgt ttctcaccgt atggaagaaa
     2221 tatttgccct cagcgatgcc atcactgtct ttaaagatgg acgttatgtc aaaaccttta
     2281 ccgatatgca gcaggttgac cacgacgccc tggtgcaggc gatggtcggg cgcgacatcg
     2341 gcgatatcta cggttggcaa ccgcgtagtt atggcgagga gcggctgcgt cttgatgctg
     2401 tgaaagcacc aggcgtgcgt acgccaataa gtctggcggt tcgcagtgga gaaattgtcg
     2461 gtctgtttgg tctggtcggg gcagggcgta gcgaattaat gaaaggcatg tttggcggta
     2521 cgcaaatcac cgccggtcag gtttatatcg accaacagcc gatcgatatt cgtaaaccga
     2581 gccacgccat tgccgcaggc atgatgctct gcccggaaga tcgcaaagcg gaaggcatta
     2641 ttcccgtgca ctccgttcgc gacaatatca acatcagtgc cagacgtaca catgtgctcg
     2701 gcggttgtgt aatcaacaac ggttgggaag aaaacaatgc cgatcaccac attcgttcgc
     2761 tcaacatcaa aacgccgggc gctgatcaac tgatcatgaa tctctcaggc ggaaatcagc
     2821 aaaaagccat tctgggccgc tggttatcgg aagagatgaa ggtcattttg ctggatgaac
     2881 ctacgcgcgg cattgatgtt ggtgctaagc acgaaatata taacgtaatt tatgcgctgg
     2941 cggcgcaggg tgtggcggtg ctgtttgcct ccagcgactt acctgaagtc ctcggcgttg
     3001 ccgaccggat tgtggtgatg cgggaaggcg aaatcgccgg tgaattgtta cacgagcagg
     3061 cagatgagcg tcaggcactg agccttgcga tgcctaaagt cagccaggca gttgcctgag
     3121 taaggagagt atgatgtctt ctgtttctac atcgggttct ggcgcaccta agtcgtcatt
     3181 cagcttaggg cgtatctggg atcagtacgg catgctggtg gtgtttgcgg tcctttttat
     3241 cgcctgtgcc atttttgtcc cgaactttgc cacctttatt aatatgaaag ggttgggtct
     3301 ggcaatttcc atgtcgggga tggtggcttg tggcatgttg ttctgccttg cttccggtga
     3361 ctttgacctt tctgtcgcct ccgtaattgc ctgtgcgggt gtcaccacgg cggtggttat
     3421 taacctgact gaaagcctgt ggattggcgt ggcagcgggt ttgttgctgg gcgttctctg
     3481 tggcctggtc aatggctttg ttattgccaa actgaaaata aatgccctga tcacgacact
     3541 ggcaacgatg cagattgttc gaggtctggc gtacatcatt tcagacggta aagcggtcgg
     3601 tatcgaagat gaaagcttct ttgcccttgg ttacgccaac tggttcggtc tgcctgcgcc
     3661 aatctggctc accgtcgcgt gtctgatcat ctttggcttg ctgctgaata aaaccacctt
     3721 tgggcgtaat accctggcga ttggcgggaa cgaagaggcc gcgcgtctgg cgggtgtacc
     3781 ggttgttcgc accaaaatta ttatctttgt tctctctggc ctggtatcag cgatagccgg
     3841 aattattctg gcttcacgta tgaccagtgg gcagccaatg acgtcgattg gctatgagct
     3901 gattgtgatc tccgcctgcg ttttaggtgg cgtttctctg aaaggcggca tcggaaaaat
     3961 ctcatatgtg gtggcgggta tcttaatttt aggcaccgtg gaaaacgcca tgaacctgct
     4021 taatatttct cctttcgcac agtacgtagt tcgcggctta atcctgctgg cagcggtgat
     4081 tttcgaccgt tacaagcaaa aagcgaaacg cactgtctga tgcttttttc cacaacaatt
     4141 tagcgttttt tcccaccaca gccagccgcc acaacggttg gctgttcttc gttgcaaatg
     4201 gcgacccccg tcacactgtc tatacttaca tgtctgtaaa gcgcgttctg cgcaacacaa
     4261 taagaaaaga gaaggaggag aaccgggtga cagaaccgtt aaccgaaacc cctgaactat
     4321 ccgcgaaata tgcctggttt tttgatctcg atggaacgct ggcggaaatc aaaccgcatc
     4381 ccgatcaggt cgtcgtgcct gacactattc tgcaaggact tcagctactg gcaaccgcaa
     4441 gtgatggtgc attggcattg atttcagggc gctcaatggt ggagcttgac gcactggcaa
     4501 aaccttatcg cttcccgtta gcgggcgtgc atggggcgga gcgccgtgac atcaatggta
     4561 aaacgcatat cgttcatctg ccggatgcga ttgcgcgtga tattagcgtg caactgcata
     4621 cggctatcgc ccagtttccc ggtgcggagc tggagacgaa agggatggcc tttgcgctgc
     4681 attatcgtca ggctccgcag catgaagacg cattaatgac attagcgcaa cgtattactc
     4741 agatctggcc acaaatggcg ttacagcagg gaaagtgtgt tgtcgagatc aaaccgcgag
     4801 gcaccagtaa aggtgaggcg attgctgcct ttatgcagga agctcccttt atcgggcgaa
     4861 cgcctgtatt tctgggcgat gatttaaccg atgaatctgg cttcgcagtc gttaaccggc
     4921 tgggcggaat gtcagtaaaa attggcacag gcgcaactca ggcatcatgg cgactggcgg
     4981 gcgtgccgga tgtctggagc tggcttgaaa tgataaccac cgcattacaa caaaaaagag
     5041 aaaataacag gagtgatgac tatgagtcgt ttagtcgtag tatctaaccg gattgcacca
     5101 ccagacgagc acgccgccag tgccggtggc cttgccgttg gcatactggg ggcactgaaa
     5161 gccgcaggtg gactgtggtt tggctggagc ggtgaaacag ggaatgagga tcagccgcta
     5221 aaaaaggtga aaaagggtaa cattacgtgg gcctctttta acctcagcga acaggacctt
     5281 gacgaatact acaacaaatt ctccaatgcc gttctctggc ccgcttttca ttatcggctc
     5341 gatctggtgc aatttcagcg tcctgcctgg gatggctatc tacgcgtaaa tgcgttgctg
     5401 gcagataaat tactgccgct gttgcaagac gatgacatta tctggatcca cgattatcac
     5461 ctgttgccct ttgcgcatga attacgcaaa cggggagtga ataatcgcat tggtttcttt
     5521 ctgcatattc ctttcccgac gccggaaatc ttcaacgcgc tgccgacata tgacaccttg
     5581 cttgaacagc tttgtgatta tgatttgctg ggtttccaga cggagaacga tcgtctggcg
     5641 ttcctggatt gcctttctaa cctgacccgc gtcacgacac gtagcgcaaa aagccatacg
     5701 gcctgcggca aagcgtttcg aacggaagtt tacccgatcg gcattgaacc gaaagaaata
     5761 gcgaaacagg ctgccggacc actgccgcca aaactggcgc aacttaaagc ggaactgaaa
     5821 aacgtacaaa atatcttttc tgtcgaacgg ctggattatt ccaaaggttt gccggagcgt
     5881 tttctcgcct atgaagcgtt gctggaaaaa tatccgcagc atcatggtaa aattcgttat
     5941 acccagattg caccaacgtc gcgtggtgat gtgcaagcct atcaggatat tcgtcatcag
     6001 ctcgaaaatg aagctggacg aattaatggt aaatacgggc aattaggctg gacgccgctt
     6061 tattatttga atcagcattt tgaccgtaaa ttactgatga aaatattccg ctactctgac
     6121 gtgggcttag tgacgccact gcgtgacggg atgaacctgg tggcaaaaga gtatgttgcc
     6181 gctcaggacc cagccaaccc gggcgtactt gttctttcgc aatttgcggg agcagcaaac
     6241 gagttaacgt cggcgttaat tgttaatccc tacgatcgtg acgaagttgc agctgcgctg
     6301 gatcgtgcat tgactatgtc gctggcggaa cgtatttccc gtcatgcaga aatgctggac
     6361 gttatcgtga aaaacgatat taaccactgg caggagtgct tcattagcga cctaaagcag
     6421 atagttccgc gaagcgcgga aagccagcag cgcgataaag ttgctacctt tcctaagctt
     6481 gcgtaggagc ggttaatctc ccgtaagtgg aactaacagc acatcgacct cacttgaggc
     6541 aatcactctt ttcgccgagc aggatgctcg cgaaaagaaa ctgtgattgt gattaccgca
     6601 aatcaccaaa tcgaaatgat atttgcgaca aacttccaga atatgttcgc ttaactcacc
     6661 gtaggcaata aacgtcttgt caacgggata ccccgcatcc tgaattaact tatcaagaaa
     6721 gctttgcgtt tcttcctgca tcacgctgcg taaatcttca agcatcggcg cagctaattg
     6781 attgtacatt tccgggtcgg aagcgagagt aattaaactg atgtgtccct ttactggcct
     6841 ggcgatagat accgcttttg ccagcagttg ctgactttcc ggtgtaaccg caacagcgac
     6901 aagaatattg ctatagctca taacctgttc cttattctgt gaacttcagg tgacattaaa
     6961 gctttactcc taatggagaa acgacgcaat cccaactcag tcaaacaggt aaatattagc
     7021 gtgattatta gtaaatacaa ttaat
//
LOCUS       DAZGQL010000165         7003 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.166, whole genome shotgun sequence.
ACCESSION   DAZGQL010000165 DAZGQL010000000
VERSION     DAZGQL010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7003
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.166"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(20..2803)
                     /locus_tag="ACHKIR_004805"
     CDS             complement(20..2803)
                     /locus_tag="ACHKIR_004805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFO9992438.1"
                     /translation="MRNLCFLLMLVATLLLPGRLIAAALPQDEKLITGQLDNGLRYMI
                     YPHAQPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPSNKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFIEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNAQDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNEVAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQITAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIAQFTA
                     ADALAADRQLFSSPADITFVIVGNVAEDKLLPLITLYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLETRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
     gene            complement(2860..5232)
                     /locus_tag="ACHKIR_004806"
     CDS             complement(2860..5232)
                     /locus_tag="ACHKIR_004806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFO9992439.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHVFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSSVKLGYRTTRSDWLTSHIDENNKSAFNQGSSGSTYYSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDFTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDISGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADRISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQDLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHISQVDVNPQ
                     IVFSYIKSKGNLSLNNGYEESNTDDNRVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLTWQEARKARIILGKTNAQYINEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            complement(5270..6955)
                     /locus_tag="ACHKIR_004807"
     CDS             complement(5270..6955)
                     /locus_tag="ACHKIR_004807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFO9992440.1"
                     /translation="MITIPITFCMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQAELIALSNAEYLQRQELSDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVADASIRTPDNKIILENLNFHVSP
                     GKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDISSPADSWYVSQTPLIKTGLLKEIICK
                     ALPLSVDDKSLSEVLHQVGLGKLAARIHDHDRWGDILSSGEKQRIALARLILRRPKWI
                     FLDETTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQPGVWNLADDICDISAVI"
BASE COUNT         1809 a         1603 c         1578 g         2013 t
ORIGIN      
        1 tgtgttgacg gggctgttat tattttggca ataatactcc ggtataggta ttcaccggat
       61 gagaaagata ttgtttaacg gcagtgttaa cattctctac cgtcatttgt ttcaataatt
      121 gctcctgctc agtccatgct gcaggatcgt catattgaat aagactattt acaatggtgt
      181 tcgctaattg ttgaacgcta cgctgttgga tatcgaggct gcgctgaacg ttttgctggt
      241 attcgttcag ttcttgctca ctgatccctt tagccagacg cttaaccatc acttcattcg
      301 ctaacgttaa cagttcatca tgtcgttccg gttgacaagt aaaagccagc aaatgactga
      361 tatctttggc ctgaggatca accgagaggc gagaagaaac gctgtatgcc ccagaggcct
      421 gttcacgaat attaacgcgt agatcttttg ccagtgcgac gttaaaagca tcgagcgcca
      481 tacgcgtctc cagattaacc ggtgtccggg aatcataacg cttccactgt gaaacctgtg
      541 ccacaggttc attttgttct tttacagtaa ccgatgcgtt gtccgtcgcg cgagttaatg
      601 gtttacctgc ggctaatggc gaatcagagt gtttgattga tcctaagtaa agcgtaatta
      661 acggcaggag tttgtcttct gcgacattac cgacaatgac aaacgtgata tccgctggag
      721 atgaaaacaa ctggcgatcg gcagccagcg catctgcggc agtaaactgt gcaatctgat
      781 tttcttgcag taatttcgtg cggtcatcag catagcgcgt ctcatacatc tgctgggcga
      841 atttctccgc tggacgctgg tcgagcgttt tcaacgcctg aatttgagca ttttgtagcg
      901 atgcccaaat attatcgtta atcgtgctgt gggtgattcg ctggttaatc aactggaaac
      961 caggttcagg gttattagtc cgcgcgctaa cagagagcaa tgtattcatg ccactgactt
     1021 tactgctcat ggttaccgag ttttccgcac tccagcgttt caggctggaa gaggagagtt
     1081 caccaacgcc gcttccgctt actgctttat ttgccagcgc gataagtgat ttttgctgcg
     1141 cagggaaact taaatcgcct ttattagata cggcagtaat ttgtagcttt tgctcttcac
     1201 ccgcggattt tgccagaata accctggcac cattggaaag tgttaatgat gtcagattct
     1261 cagccagcgt ttctttgctg ctaatttccg cctgtgggtc agcgtctact gttaacgata
     1321 aatttctgcc tgggaagatg taagccgcca gctttttgtt ggcgtactct ttttccagcg
     1381 ccaggatagc cgcaggagac aatgcttttt tggcggcaac ttcattgttt accatttgct
     1441 cccaaaatgc gtcctggttc tttcttaact gctgccattt ttccgccaga ctttgcacgg
     1501 taatttgctg ccacagacgt ttcgaaagtt gatatgtctc ttccggcgac aagaacggcg
     1561 tattatttaa tgagctggat gccaggcgac tggtcagcat acgtaaatca cgctcagctt
     1621 gctgatcaac cgcatttttc agccaggtaa ggcgggtaga tttaacatca tcgagttctt
     1681 cagcagaaaa gccatgctga tcaatggttg ccaactctgc cattaatgca ttcgcagcat
     1741 cctgcatatt atcgtcttgt gcatttacac ggaaaaacaa agactgataa tcgggtgcaa
     1801 ttttgacgct gcgcgcagtg ccgccagaaa tagtcttcaa ctcgcccgac tgtatgcgtt
     1861 cttgcagacg ttggttgaac agctgaacca acatgctcca ttcagcttgt tcgataaagc
     1921 tttgctcatc gttcacttgt accattggca ggcgataata gagtgcgatg ccgttcaccc
     1981 ggttttcttt atcattgatg atattaaagc gcaggtggtt ttcggctttt gtcggccaga
     2041 cgcgattttc agctgctttg ttagacggaa gcttacttaa attatcctta atcagcgcca
     2101 gcgcttcttt actgtcaata tcgccgacca cgataaaggt catattattt ggttgatacc
     2161 agcgttgata aaattggcgc aattgtgccg gtgtgaccgt agcgacagta tccatcaggc
     2221 cgataggttc acggtctaaa ttacgggtat ttgccagcag gaaagggcgg cgcgcctgag
     2281 aggtgcgcca tttcgcatcc tgatgggcac gccattcctc agtaattacg ccgcgttcag
     2341 cgtctacttc gagtttttca aaggttgcgg cattactcca ttcactgaag attgccatca
     2401 cttgttgcag attttgtttc tgcgtagtcg gcaaactcac ctgatacact gtttcgtcat
     2461 agctggtata ggcattaaca tcgcgaccaa aacgcaggcc cattgactca aatgtttcga
     2521 tgactttatt acccggccat gtttttgtgc cgttaaacat catatgttct acaaaatgag
     2581 ccacgccgcg ctcattgtcc tcttcctgca atgaaccggt atgaatttgc agccataaat
     2641 ttacctgatc ctttggttga gcatgcggat aaatcatata tcgcaagcca ttgtccagtt
     2701 gcccggtaat taacttttca tcctgcggta aggcggcggc aatcagtcgc ccggggagca
     2761 acagagttgc cactaacatc agtaagaaac agaggtttct cataattatc tccatgcgaa
     2821 aaccgggcaa atttacccgg taaagtaaaa tccgaactat taaaatttca tgctgacatc
     2881 aagccagaaa gtacggccac tggcgtaggt cgccacgccg gtattcgttg tatcaaccgc
     2941 ggttttgcta tccagtacat tgagaatatc ggcactgatc gtcaggtttt gttgtttcag
     3001 aaattgtggc gtccaggaca agcgggtgtc ccaggtcagg ctgctatcca gtttctcgtc
     3061 aacatactgc ttgtaatctg aatattcgtt gatgtattgc gcattcgtct tacccaggat
     3121 aatgcgagct ttacgcgctt cttgccaggt cagcgtattc gcccacacca acccgctcgg
     3181 ttgatgcgtg aaatccatgt ttaaggagat ctttaatgga ttattaaaat ccgccactgg
     3241 aacgctatcg taagagacca gattaccgtt ataaaccacc cggttatcat cggtattgct
     3301 ttcctcataa ccattgttta acgacaagtt gcccttgctc ttgatatagc taaagacaat
     3361 ttgcgggtta acatctacct ggctgatatg caggggttcg gccagttcaa aactgaggtt
     3421 gaacgaatgc gttttggttt tgccgtcgtt gttatattca gtaatggtgg ttttagtcgc
     3481 gctgtcggta cgactgcttt tgctgatttg atcatgcgct tcacggtaaa catagtttgc
     3541 gcgcgcaata acgttcttac cgattttctg ctgcaacccc attgccagtt catcgttata
     3601 aggcgttttc aaatcctgat aacgcgtcag ggttttattg cctgataccg attccgtcca
     3661 gctattgcgg atatcacgta atcccatatc aagaatattc ccgccatagt aacggttata
     3721 acctgcggta atcattgagg tttgatcagc aaaaatatcc cattccgtca taaagcgcgg
     3781 ggagatattg tggtttgaca gatagttgtc atagtcatac cgcacaccgg gcattaacga
     3841 cacattccgc cagctaatgc gatccgccat atacagcgtg tagttgtcaa ttcccagact
     3901 gcctttacct ttatggtaaa tggtatggtt agttttcttt cctgcagcat taatcacata
     3961 ggattcagac tggttatggc gttcagtcca tgcatcggaa tagatgtatt ccgcgccgaa
     4021 gtagggttga tgcgaaacat cacccacggc gaatttttgc cagtccaggc gtgttttgaa
     4081 ggtgtaatta tctacagcct gggaaatgtg tcctaatcca ccacgggtgc aacgacctga
     4141 aatatcacca tatgtacatg aaagttcggt gtaccagatg tcatgatcgt gacgggtata
     4201 atcactaata tgatcccaac caacggtagt acgtagtttg gcccatgcga gctgcgtatc
     4261 catatcccat gccagaccgt aggatttatt acccatttcg cgatcagact gcgggaaggt
     4321 gctggtatta taatcacgac tggagccggt atattttaag gtaaaatcgt gggtaaagcg
     4381 gtcgctggca aaccaagtaa atttgctcaa tgcggtatcg ataacgtttt tatactgtgc
     4441 ccgaccggcg acaatgccgt cattcgaaac ataatccgcg cgggtgatat cagactggcg
     4501 gcgcgataaa ccggcggtaa cgccaaagtt atcagcgagt tcctgattaa acgacaaggt
     4561 ataaaagttc tttttaaaat ctggagagta ataagtactt cctgaagaac cttgattaaa
     4621 tgcgctcttg ttattctcat cgatatgcga tgttaaccag tccgaacgcg tagtgcgata
     4681 acccagtttc acgctgctat catcagcgtt gaagcgtttg atctttgcat caattacccc
     4741 gccattgaag cgaccgaatt caaccggcac aaaactgtca taaagcgtca cattgtccag
     4801 taagctgaca tcaagataat agccctgtga catcccgcta atattggttg cactactggc
     4861 atcggactca ttcgctgggt tcaggttatt agttgcacta ataccatcaa tcaaataggc
     4921 attctggtag ggcgacgcac cgtgaataga gattttctcc gggcgaatat ctccctggtt
     4981 caatgaggta ctttgcgttg aatccatgcg caccgcaggg ttggttctca gcagatcgct
     5041 gatattgcca ttcccggtcg gtaaacgctc gatgctttca ctggtgtaat gcgtgttgcc
     5101 gtttacgggt acagggacag gagcatagac cgtcatctct tcaaaaacat gcatgtacat
     5161 gtttttgtca tcgacggcct gcgctacatc agcgccacat aaaatgacgc caggaataag
     5221 aactcgcttc atttatttat atctccgtac agcagggctt ttattgtttt tatataaccg
     5281 cgctaatgtc acaaatatca tcagccaggt tccagacacc gggttgatgt gtaaccataa
     5341 tgacgccgct tgtgggtagt ttttcacgca ctaaacgcag taagcggata gcctcttgtt
     5401 cctcaagatg agaggtagtt tcgtcaagaa atatccattt cggacgtcgt aaaattaatc
     5461 gtgccagggc gatacgttgt ttttcgccgc tggaaagaat atcgccccag cgatcgtggt
     5521 catgaatacg cgcagccaat ttccccagac caacctgatg cagtacttcg ctcaacgatt
     5581 tatcgtctac ggacagggga agtgctttac aaattatctc tttcagtaag ccggttttga
     5641 ttaacggtgt ttgtgacaca taccaactgt cagcaggaga agaaatatca cctttaaacc
     5701 acggccagca gtgggataat gttttaagca gtgtggtttt tcccgcacca gagtaacctt
     5761 tcagtaatag ccatttgcct ggcgaaacat gaaagttcag gttctctaat atgatcttat
     5821 tatcaggagt acgaatactc gcgtcagcca cttgtaccgc atgttggcaa tttttaggct
     5881 tattcgtagg gcgctgttcg gtgagttgat ggaactcata caaacgatcg ataaccgcag
     5941 ccagttcagc aagttcgtca tatttataaa taaaccagct taaattgttc gataccagca
     6001 taaatgcctg gcgcgatttc atcagtccgc ccagattgat ctgtccgcta ataaactgcg
     6061 gcaataacag aaagtaggga agaacgctaa gcgagcgcga ataaatattc tgccagtaat
     6121 caagccaccg ctgacgattc attaaacgat gccagttctc tttaatggta tgaaaattgt
     6181 cgcttaactc ctggcgttgt agatactcag cattgcttag cgcaatcagt tctgcctgct
     6241 tattatgctg cacaagatta gttcgaaatg tcgcttcgct acgttgtttt tccacattaa
     6301 gcggacgaat acgtttacca accttatggg taaataaagt tccaccgatc acaatgagca
     6361 caacggtata gaccatatac ccctggatat tccattctgt tccaccaaca gtgaatgaga
     6421 gcgtacccgc gctttgccaa agaataacgg taaaggtgat caacatgcta agtgactgga
     6481 tgaagccaaa tgaaaggctg agcgttttac tgatcagtaa gagaatgtcc tcagcgatac
     6541 gttggtcagg gttatctgta tttttatgct caccgtagat ctgcgtgaag tagtagtttt
     6601 tatctgcaaa ccagcgatta aggtagtaat ctgttaacca ttcgcgccag cgaatagtca
     6661 gtagtttaat tagccatgtt ttgtttacgg atattaatac aaagatcccc agcagggcag
     6721 ggaaccaaag gacaagctgc caaagcttat cggtttcttt ctggcttagc gcattgaaaa
     6781 aatcattatt ccagtcgttt aaccagacct gaattttaac cacgccaagg atcatggcga
     6841 ggataataat gattaacaac actgaagttt tattattctt acgcagccaa aatggcttca
     6901 gcagacagag atactttgct atcaacatac aaaacgtaat gggaatggtt atcattagcg
     6961 aaaattgatt gtgccatcca atgattatct atggcaatga ttt
//
LOCUS       DAZGQL010000166         6982 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.167, whole genome shotgun sequence.
ACCESSION   DAZGQL010000166 DAZGQL010000000
VERSION     DAZGQL010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6982
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.167"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..131
                     /locus_tag="ACHKIR_004808"
     CDS             <1..131
                     /locus_tag="ACHKIR_004808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFO9992441.1"
                     /translation="RHIAINILTNDKVFKAGLRRKLRKAAMDRNYLASVLAGSGLS"
     gene            329..2566
                     /gene="nrfB"
                     /locus_tag="ACHKIR_004809"
     CDS             329..2566
                     /gene="nrfB"
                     /locus_tag="ACHKIR_004809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-3',5'-guanylate-activated
                     glycosyltransferase NrfB"
                     /protein_id="HFO9992442.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGDGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMIQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRALKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQAGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNPEQVAQLES
                     ENEGE"
     gene            2553..5525
                     /gene="nfrA"
                     /locus_tag="ACHKIR_004810"
     CDS             2553..5525
                     /gene="nfrA"
                     /locus_tag="ACHKIR_004810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HFO9992443.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLIEN
                     TPLFTTDAQEKSWLYLLSKYSGNPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYTVSVATRNKAEALRLARLLYQQEPANLTRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPNAWQHRAVAYQAYQVEDYATALAAWQKISLHGMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGNNALYWWLHAQRYIPGQPELALSDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNTQAALGYALWDSGDIAQSREMLEQAHKGLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRH
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPRKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGIEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            5526..6416
                     /gene="ybcH"
                     /locus_tag="ACHKIR_004811"
     CDS             5526..6416
                     /gene="ybcH"
                     /locus_tag="ACHKIR_004811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HFO9992444.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRALLFKRAAAAQQAGLKLIVGLNADPEFFIH
                     QKQSSAALESYLNRLLAADLQQARLWSAVPGVTPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAQSILEY"
BASE COUNT         1735 a         1810 c         1796 g         1641 t
ORIGIN      
        1 tacggcacat cgctattaat attttgacga atgataaggt attcaaggca gggttaagac
       61 gtaagttgcg aaaagcagcc atggacagaa actatctcgc gtcagtcctt gctgggagcg
      121 ggctttcgta atctttccct gcgttaacct cccctccttg acaccacgct tttgtctaca
      181 ttctacgcaa acggcaactt ttataaccag tgtaaaaata acgtgccgca atagtcatac
      241 ctattaatgg taaaaagctg tcacaattca taaaaaacct taatatacgc caccctaaac
      301 ataaccagcg ttaatgtaag gtttttgtgt ggactggctt cttgatgttt ttgctacctg
      361 gctctacggc ttaaaagtaa tcgcgataac gttagcggtc atcatgttca tcagcgggct
      421 ggacgatttt tttattgatg tcgtctactg ggtacgccgc attaaacgca agttgagtgt
      481 ttatcgccgc tacccgcgaa tgagttaccg cgaactgtat aaaccagatg aaaaaccgtt
      541 agcgattatg gttccggcgt ggaatgaaac gggcgtcatc ggcaatatgg ccgagctggc
      601 ggcgaccacg ctcgactacg aaaactatca tatctttgtt ggcacctacc ccaacgaccc
      661 cgatactcag cgtgatgttg acgaagtgtg cgctcgcttc ccgaacgtgc ataaggtagt
      721 ctgcgcgcgt cctggcccca ccagcaaagc cgactgtctg aacaacgtgc tggacgccat
      781 cacccaattt gaacgtagcg ccaatttcgc ttttgctggt tttattctgc atgacgccga
      841 agatgtgatt tcaccgatgg aattgcgtct gttcaactat ctggtcgagc gtaaagatct
      901 gattcagatc ccagtgtatc cgttcgaacg cgaatggacg cacttcacca gcatgaccta
      961 cattgatgag ttttcagaac tgcatggcaa agatgttccg gtgcgtgaag ccctcgccgg
     1021 acaggtgccc agcgcaggcg tcggcacctg tttcagccgc cgcgccgtga ccgcactgtt
     1081 agctgacggt gacggtattg ctttcgacgt gcagagtctg actgaagatt acgacattgg
     1141 tttccgcctg aaagaaaaag gtatgacgga aatttttgtc cgttttccgg tggtggacga
     1201 agccaaagaa cgcgagcagc gtaaattttt acagcacgcg cggacgtcaa acatgatctg
     1261 cgtgcgcgaa tatttccccg ataccttttc gactgcggtt cgacaaaaat ctcgctggat
     1321 catcggcatt gttttccagg gctttaaaac ccacaaatgg acctccagcc tgacgctgaa
     1381 ctactttctc tggcgcgacc gcaaaggggc aatcagtaac tttgtcagct tcctcgcgat
     1441 gctggtgatg atccagcttt tgctgttact ggcgtatgaa agtttgtggc ccgatgcctg
     1501 gcatttcctt tctattttca gcggcagcgc atggttaatg accctgctgt ggctaaactt
     1561 tggtttgatg gttaaccgca tcgtgcagcg ggtgattttt gtcactggct actacggtct
     1621 gacgcagggg ctgctatctg tcctgcgtct tttctggggc aacctgatta acttcatggc
     1681 caactggcgc gcgttaaaac aggtacttca acacggcgat ccacgtcgcg tcgcgtggga
     1741 taaaacaacg catgacttcc ccagcgtgac tggcgatacc cgctcgttgc gcccgttagg
     1801 ccaaatcctg ctggaaaatc aggtcatcac tgaagaacaa ctcgatacag cactgcgtaa
     1861 tcgcgtcgaa ggtctacgcc tgggcggttc aatgctgatg caggggctga ttagcgccga
     1921 acagctggca caggcgctgg cagagcaaaa cggcgtggcg tgggagtcga tcgacgcctg
     1981 gcagatccct tcatcactga ttgccgaaat gccggcttcc gtggcgctgc attatgcagt
     2041 gctgccgctg cgtctggaaa atgatgagtt aattgtcggc agtgaagatg gtattgaccc
     2101 ggtttcgctg gcagccctga cgcgtaaagt cggacgcaaa gtgcgttacg tcattgttct
     2161 gcgcggacaa attgtcacgg ggttacgtca ctggtatgca cgccgacgcg gtcacgaccc
     2221 gcgggcaatg ttgtacaatg cggttcagca tcagtggctc acggaacagc aggccggtga
     2281 aatctggcgg caatatgtgc cgcatcagtt cctgttcgcc gaaatactga ccacgctcgg
     2341 tcatattaat cgttcagcaa ttaacgtgtt gttattgcgc catgaacgca gttctctgcc
     2401 gctcggcaag tttttggtca ccgaaggcgt tatcagccag gaaacgttgg atcgcgtcct
     2461 gacaattcaa cgcgaattac aagtttcgat gcaatcacta ttactcaaag caggtttaaa
     2521 cccagaacag gttgcgcaac tggagtccga aaatgaagga gaataacctt aatcgcgtca
     2581 tcggatggtc tggtttactg ctgacgtctt tattgagcac cagcgcactc gcagacaata
     2641 tcggcaccag cgcagaagag ctggggctga gcgattatcg ccattttgtt atttatcccc
     2701 gtctcgataa ggcgctgaag gcacagaaaa ataacgacga agcaaccgcc atccgcgaat
     2761 ttgaatatat acaccaacag gtgccggata atattccgct gactttatac cttgcggaag
     2821 cctatcgcca ttttggtcat gatgaccggg cgcggctgtt gcttgaggat caactgaaac
     2881 gtcacccagg agatgcccga cttgagcgca gtctggcggc tattccagtt gaagtgaaaa
     2941 gcgttacgac tgttgaagaa ctgcttgccc agcaaaaagc gtgcgatgct gcgccgaccc
     3001 tgcgttgtcg cagtgaagtc gggcagaatg ccctgcggct ggcacaatta cctgtcgcca
     3061 gagcgcaact gaacgatgcg acgtttgctg catcgccgga aggaaaaacg ctgcgaaccg
     3121 atctgctgca acgggcaatc tacctgaaac aatggtccca ggcagatacg ctatacaatg
     3181 aagcacgcca gcagaacaca ttaagcgcgg cagaacgccg tcagtggttt gatgtgcttc
     3241 ttgccgggca gctggacgat cggatcctgg cgctgcaatc acaggggatc ttcaccgatc
     3301 ctcagtcata tattacttac gcgaccgcgc tggcttatcg tggcgaaaaa gcacgcctcc
     3361 agcattatct cattgaaaat acgccgctat ttaccacgga cgcacaagag aaaagttggc
     3421 tctatctgtt atctaaatac agcggcaacc ccgttcaggc gttggcgaat tatacggtac
     3481 agtttgccga caaccgccag tatgttgttg gcgcgacgct accggtgctg ttaaaagaag
     3541 gtcagtacga cgcagcgcaa aaactgctcg ccaccctccc cgccaatgaa atgcttgagg
     3601 agcgttatac cgttagcgtg gcgacccgta acaaggctga agctctgcgt ctggcacgat
     3661 tgctgtatca gcaagaaccg gcaaatctta cccgcctgga tcaactgacc tggcaactga
     3721 tgcagaacga gcagtcacgt gaagctgccg atttattgct gcaacgctat cctttccagg
     3781 gcgatgcgcg tgtcagccag actttaatgg cgcgactggc gtctctgctg gaaagtcatc
     3841 cttacctggc aactccggcg aaggtggcga ttttatcgaa acccttaccg ctggcggagc
     3901 aacgtcagtg gcaaagtcag ttgccgggta ttgcagataa ttgcccggca atagttcgct
     3961 tgctgggcga tatgtcgcct tcctacgatg ctgccgcctg gaaccgtctg gcaaagtgtt
     4021 atcgggacac gctacccggt gtggcgttgt atgcatggct tcaggccgaa caacgacaac
     4081 cgaacgcctg gcaacatcgt gcggtagcct atcaggcgta tcaggttgag gactacgcca
     4141 ccgcactggc ggcctggcag aaaatcagtc ttcacggcat gagcaatgag gatctgcttg
     4201 ctgctgccaa taccgcccag gcggcaggaa atggtgcggc tcgcgatcgc tggcttcagc
     4261 aggcagaaca acgtgggctg ggaaacaatg ccctctactg gtggctgcat gcgcaacgtt
     4321 acattcctgg tcagccggaa ctcgcactga gcgatctcac gcgctcaatc aatattgcgc
     4381 cttctgccaa cgcttacgtt gcgcgggcga caatttatcg ccaacgtcat aatgtcccgg
     4441 cggcggtgag tgatttgcgc gccgctctgg aactggaacc gaataatagc aacacccagg
     4501 cagcgctcgg ttacgccttg tgggatagcg gtgatatcgc acagtcgcgg gaaatgctcg
     4561 aacaggcgca taaagggcta ccggacgatc cggcacttat ccgacaactg gcctacgtga
     4621 accagcgtct ggatgacatg cctgcgacgc agcactacgc ccgactggtg attgatgaca
     4681 ttgataatca ggcgctgata accccactga ccccagagca aaatcagcaa cgcttcaatt
     4741 tccgccgtct gcatgaggag gtcggtcgcc actggacgtt cagtttcgat tcttctattg
     4801 gcttgcgttc cggcgcaatg agtaccgcta acaataatgt cggcggcgca gcgccaagga
     4861 aaagctatcg tagctacggg caactggaag ccgagtaccg catcggacgc aatatgctgc
     4921 tggaaggcga cctgctctca gtttatagcc gcgtctttgc cgataccggt gaaaacgggg
     4981 tgatgatgcc ggtgaaaaat ccgatgtccg gcaccggtct gcgctggaag ccgctgcgcg
     5041 atcagatctt tttcctcgcc gtcgaacagc agttgccgct gaacggacaa aatggcgcat
     5101 ccgataccat gctgcgcgcc agcgcctcat tctttaatgg cggcaaatac agcgacgaat
     5161 ggcacccgaa cggttcaggc tggtttgccc aaaacctgta cctcgatgcg gcgcaatata
     5221 tccgccagga tattcaggcg tggacggcag attatcgagt cagctggcat cagaaggtgg
     5281 caaacgggca gactattgag ccttatgctc acgttcagga caacggttat cgtgataaag
     5341 gcactcaggg cgcgcagctt ggcggtgtcg gggtccgctg gaatatctgg accggcgaga
     5401 cgcactacga cgcctggccg cacaaagtca gtctcggcat cgaatatcaa cataccttta
     5461 aggcgattaa tcaacgtaac ggagagcgca acaacgcgtt tctcaccatt ggagtgcact
     5521 ggtaaatgcg taagttcatt ttcgtattgt tgacactgct tttggtcagc cctttttcct
     5581 ttgcgatgaa aggtattatc tggcaaccac aaaaccgtga tagtcaggtt accgataccc
     5641 agtggcaggg gctgatgagt cagttacgtt tgcaaggctt cgatacccta gttttgcaat
     5701 ggacccgtta cggcgatgca ttcacccagc cagaacagcg cgcgttattg tttaagcgtg
     5761 ccgcagctgc gcaacaggct ggcctgaagc tgattgtcgg gctgaacgcc gatccggaat
     5821 tttttataca ccagaaacag tcctccgcag cgctggaaag ctatcttaat cgcctgctgg
     5881 ctgccgatct ccagcaagcc agattatgga gcgccgtgcc tggcgtaacg ccggatggct
     5941 ggtacatcag cgcggaaatt gacgacctga actggcgcag cgaagccgcc cgtcaacctt
     6001 tgctaacatg gttaaacaac gcgcagcggt tgattagcga tgtttcagca aaaccggttt
     6061 atatcagtag ttttttcgcc ggaaacatgt cgccagatgg ctatcgccaa ctgctggaac
     6121 aagttaaagc aaccggtgtt aatgtctggg tacaggatgg cagcggcgtg gataaactga
     6181 ccgctgaaca gcgtgaacgt tatctacagg ccagcgccga ttgccaaagt tccgccccag
     6241 caagcggcat tgtttatgaa ctgtttgtcg ctggtaaagg taaaacgttt acagcgaaac
     6301 cgaaaccgga tgcagagatc gcctcgctgt tagcgaaacg ttcctcttgc ggtaaagaca
     6361 cgctctattt ctctctgcgc tatttgcccg tcgcgcagag tatccttgag tattaatgat
     6421 taaagggagc aacttagctt gctcctttcc ttttcacttt cctaatcaaa atcctggcaa
     6481 gatgaattgt ttagcagaat ttctgttttc attttattcg tctgacgtgt tccaattatt
     6541 tagcaaaacc gcgacaacaa agtgatggtg taatttgtaa aagggaacaa ataaatggcg
     6601 aggaaatggc tcgccctgca ggattcgaac ctgtagccca cgacttagaa actccaggaa
     6661 cgacatttta aatcaacaaa ttaccgcgcc atctctgcgt tcacacgttc caccacctca
     6721 aaatatgtaa aaccttgcaa gccattgcga ggccttatgt gtctcagttt tgttcctctt
     6781 ttttatacta aaaaacacag caattgagga taaacctcat gctattatcg ctaatatgca
     6841 tctaaaggca tggtacttga atagataaaa actccacaaa gaaaaaaaat aattagccca
     6901 taaaaccaca atgcaaaaca caaacagaca attaaactaa aaaactgaca gtgcattgtg
     6961 acaaaaattc aatgttaaac aa
//
LOCUS       DAZGQL010000167         6926 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.168, whole genome shotgun sequence.
ACCESSION   DAZGQL010000167 DAZGQL010000000
VERSION     DAZGQL010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6926
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.168"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(98..700)
                     /gene="fimB"
                     /locus_tag="ACHKIR_004812"
     CDS             complement(98..700)
                     /gene="fimB"
                     /locus_tag="ACHKIR_004812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
                     /protein_id="HFO9992445.1"
                     /translation="MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIH
                     GFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTHPLLNKEIQALKNWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
                     IQDYLGHRNIRHTVWYTASNAGRFYGIWDRARGRQRHAVL"
     gene            2152..2868
                     /gene="nanC"
                     /locus_tag="ACHKIR_004813"
     CDS             2152..2868
                     /gene="nanC"
                     /locus_tag="ACHKIR_004813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HFO9992446.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            2888..3994
                     /gene="nanM"
                     /locus_tag="ACHKIR_004814"
     CDS             2888..3994
                     /gene="nanM"
                     /locus_tag="ACHKIR_004814"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HFO9992447.1"
                     /translation="MNKTITALTIIMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGDNLKWNKLAPVASPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRVYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            4059..5039
                     /gene="nanS"
                     /locus_tag="ACHKIR_004815"
     CDS             4059..5039
                     /gene="nanS"
                     /locus_tag="ACHKIR_004815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFO9992448.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHPRIKQL
                     ARFAHTHPGGPSCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGVLIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTCDYSSHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHAYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSAAARRGIISDRFVEAILRFWRER"
     gene            complement(5047..5697)
                     /locus_tag="ACHKIR_004816"
     CDS             complement(5047..5697)
                     /locus_tag="ACHKIR_004816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001586610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFO9992449.1"
                     /translation="MNKLPDHLPRRYIIEAIHLWDAGETHQFQPARLYEIEYHGRRYP
                     SKAIAGIAATLMTGTQFTPADFTGGIKSKCVKLLIEQGFHIHQDKAAAAPIADVLFPD
                     ELPSQETYLEGAAIKVTVNRYERDKKARDKAVEHHGCQCNVCGVDLVKIYGDIAEGFI
                     HMHHLVPLSAIKEDYQLDPVNDLLPVCPNCHAMLHRRKPPFSPEELKALMDANKSN"
     gene            5834..5977
                     /locus_tag="ACHKIR_004817"
                     /pseudo
     CDS             5834..5977
                     /locus_tag="ACHKIR_004817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_080685909.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
     gene            complement(6685..6768)
                     /locus_tag="ACHKIR_004818"
                     /pseudo
     CDS             complement(6685..6768)
                     /locus_tag="ACHKIR_004818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025205684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
BASE COUNT         2064 a         1458 c         1501 g         1903 t
ORIGIN      
        1 cacttacaat aacatcaatt aataaatggt taatcatttt tgatatatcg taagaataat
       61 gtagttttta acaccatccc tggtatctca actatctcta taaaacagcg tgacgctgtc
      121 tccctctggc tctatcccag atgccgtaaa aacgccctgc attgctggcg gtataccaga
      181 cagtatgacg aatattgcga tgcccaagat aatcctggat aagtcgcgta tctattccca
      241 tattcgccaa agcaaaacca cacgaatggc gtaacatgtg cggatgaatc tccagtgaca
      301 acccggcatt accaccggaa gtcgagataa tatggtaaaa ctgttgccga gaaagtggat
      361 tccccttacg tgataagaat acccactcgc tctcagcatg cggataagaa gtacggatac
      421 tcaaccagtt ttttaaagcc tgaatttctt tgttcaatag cgggtgcgtt gttgaaaagc
      481 ctttttttaa tcgatggata tatatacact ttgccttaag atcaatatcc gaaatcctca
      541 atcggcaaat ttcactcgcc cggaaaccat gaataaaaca aagcaaagtc agacaataat
      601 tacgtgctgc atgaggcccg gtatttgctg ctttaaggag tgattcgatt tcactatggg
      661 tcaggaagtt cctttttttg ttatcagcct tattcttcat cgttttccct tataattaca
      721 gacgcgcact agctgtgctg ggttagcaat aatccaacat ttttatcctg attatgttta
      781 taaaagcgaa cgtttgctta ataactaacg taagtggacc agttcttctg agcgaactta
      841 aatggagtag cgatagttac ttataataac tttgccatac aaacgccatg ctcttgcatg
      901 ctatgtacct ttatatattt atcaatctga tacgaatcga ataataaccc tcatcattaa
      961 tgcaactaat cttatctata tatcatgtga tatgttctgt aacaagtaaa atcaacataa
     1021 aaaattcagc aaaatttaat attcgttcaa ctagccgtct cacatatgac attattagtc
     1081 aactgcattt gccacaaaat ggctatttca tggaacgcag taatttctta gtggctatta
     1141 tcatgctaat tcttagatcc ctagcgatta ttcctgcctg gttcgtcagc ttaggccatg
     1201 agattgttac attccctttt atcctggaga tatgggggaa cgcaggcgac gctttttcat
     1261 ctacatccgt tggtaactat ggctttggtc actacgactt aaaccttcct caatcacagc
     1321 tattcgccgg gcgatacggc aaacaaacta agtgaaacat catcctgaaa acaccaacat
     1381 caacaagcct ctccagatcg acatcaggag tgaccagtta caagccacaa accaagaaac
     1441 atatttcatg atgagaatta tgctcagcct gatggcgggt aaacacttat tttcaccaag
     1501 ctcactgagt tagcttgcaa agctttcttt gtttagattc taaataaatt ttttataaaa
     1561 cagacagtta aaataaaatc tttaattttt gaagaccacc gcaagtgttc gtctggcttc
     1621 acatggcatc tgcctcttag aaaaagatcg acatattttg tgacacgaat tgcaaatctc
     1681 gttttgttgt atggattgcg tgatttttga tctggtataa caggtataaa ggtgcaccgc
     1741 gatagtcaat gagacagggc atctcgcaat ctatggcaaa catcacttca gttctttctc
     1801 atcgggtgat gaaaacgcac ttcagtctga aaggaatatg aaaatgagat caacagacac
     1861 tctattttat gactctgggt aaaatggatt gagtaagtga tatagcttac gaacattcaa
     1921 atcaattaaa catcagaaga gattttatac tcaggtattt aatctggttc tctgtttatt
     1981 taaataatgt ggaaagagaa ttttcacagg agaccttata caaaaaatat aaaataagct
     2041 acccgttgcc aaagacacta taagcctggc aaaaaatatt acacaacata aatgctaatt
     2101 gtttatgcgg gctttgtatt gctttctgta tcctacaaat gagtgaaatt tatgaaaaag
     2161 gctaaaatac tttctggcgt attattactg tgcttttcgt caccattaat ttctcaggct
     2221 gcgacactgg acgtacgtgg tggatatcgt agcggaagcc atgcctatga gactcgactc
     2281 aaagtcagtg agggatggca aaatggatgg tgggcaagca tggaaagtaa tacctggaat
     2341 accattcatg ataataaaaa ggaaaatgcc gcactcaatg atgttcaggt tgaagttaat
     2401 tacgcgatta aacttgatga tcaatggacg gtgcgcccgg gaatgttaac gcattttagc
     2461 agcaacggca cacgctacgg accctacgta aaactgtcct gggacgcgac aaaagatctt
     2521 aattttggca ttcgctatcg ttacgactgg aaagcttacc gacaacaaga cttatccggt
     2581 gatatgtctc gtgataacgt tcatcgttgg gatggatatg tcacttacca tattaatagt
     2641 gatttcacct tcgcatggca aacgacgcta tacagcaaac agaatgatta tcgctatgca
     2701 aaccacaaga aatgggcgac ggaaaatgcc tttgttctac aataccatat gacgcccgat
     2761 attacgccat acatagaata tgactacctt gaccgtcagg gtgtttacaa cggcagagat
     2821 aatttatcgg aaaacagtta tcgcattggt gtgtcattta aactgtagta gacaggagac
     2881 agtcacaatg aataaaacaa taacggcgct tactatcata atggcttcat ttgccgcaaa
     2941 cgcgtctgta ttaccggaaa ctcctgtgcc atttaaaagt ggtaccggag caattgataa
     3001 cgacactgtc tacattggtt taggtagcgc aggtacggca tggtacaagc tggatacaca
     3061 ggccaaagat aaaaaatgga cagcgttagc tgcattccct ggcggaccga gagatcaagc
     3121 aacctctgca tttattgatg gcaatctgta tgtgtttggc ggcattggca aaaacagcga
     3181 gggcttgact caagtattta atgacgtaca caaatacaac cccaaaacca atagctgggt
     3241 taaattgatg tcgcacgcgc cgatgggcat ggcgggccat gtgacttttg tacacaacgg
     3301 caaggcttat gttactggcg gtgttaacca gaatatcttc aatggctatt ttgaagatct
     3361 caacgaagct ggaaaagatt caaccgctat agataaaatc aacgcccact attttgacaa
     3421 aaaagcagaa gattatttct tcaataagtt tctgttgtct tttgatccct caacacagca
     3481 atggagttac gctggcgaat ctccctggta cggaacggct ggtgcggcgg ttgtgaataa
     3541 aggtgataaa acctggctta ttaatggcga agccaaacca ggattgcgaa cggatgccgt
     3601 atttgaactt gacttcaccg gtgataattt aaaatggaat aagcttgctc ccgtcgcatc
     3661 accagatggc gtcgctggcg gttttgcggg gataagcaat gattctctta tatttgccgg
     3721 aggggccgga ttcaaaggtt cgcgggaaaa ttaccaaaac ggtaagaact atgcgcatga
     3781 aggcctgaaa aaatcatata gcactgatat tcatctttgg cataacggga aatgggataa
     3841 atctggtgaa ttatcgcaag gtcgggtcta cggagtatca ttgccctgga ataatagttt
     3901 attaattatt ggcggtgaaa ctgcaggcgg caaagcggtg acggattcag ttttaatttc
     3961 tgtgaaggat aataaagtca cagtacaaaa ctaacgcttc agggcctcgg taaaggggcc
     4021 ctgctatcaa tttgtcattt gtattacgcg aaaattatat gaacgcaata atatcgcccg
     4081 attattacta tgttcttacc gttgctggtc agtctaatgc catggcgtat ggcgaaggac
     4141 tgccattacc ggacagggaa gatgcgcctc atcccagaat taaacaatta gcgagatttg
     4201 cgcatacgca tcccggaggc ccgtcatgtc actttaacga cattattcca ctgactcact
     4261 gcccacacga tgttcaggat atgcagggtt atcaccatcc tctggcaaca aatcatcaaa
     4321 cacagtacgg caccgttggc caggcactgc atattgcacg gaaattactg ccctttattc
     4381 ctgataacgc aggggttctc atcgttccgt gttgccgtgg cggatcggct tttaccgcgg
     4441 gcagcgaagg gacatattca gaacggcacg gagccagcca tgatgcttgt cgttggggaa
     4501 cggatactcc gctgtaccag gatttagtca gcagaacgcg agccgcactg gcaaaaaatc
     4561 cgcagaacaa attcctcggc gtatgctgga tgcaaggcga atttgactta atgacctgtg
     4621 actactcgtc acaccctcaa cactttaatc atatggttga agcctttcgt agggatctaa
     4681 aacaatacca ttctcagctt aataatatta ctgacgcacc gtggttttgc ggcgatacca
     4741 cctggtactg gaaagaaaat ttccctcatg cgtatgaagc gatttatggc aattatcaaa
     4801 ataatgtttt agccaatatt attttcgtcg acttccagca acaaggtgaa agaggactga
     4861 cgaacgcgcc tgatgaagat ccggacgatt taagcacggg atattacggt tcagcgtacc
     4921 ggtcaccgga gaactggacg acggcactgc gaagcagtca tttcagcgcg gcagcccgtc
     4981 gggggattat ttctgacagg tttgtagaag caattttgcg gttttggcgc gaaaggtgag
     5041 cgttcattaa tttgatttat ttgcatccat caacgctttt aactcctccg gtgaaaacgg
     5101 tggtttgcgt cggtgcagca tcgcatggca attagggcac acaggcagca aatcattaac
     5161 tggatccagc tggtaatcct ctttaatggc tgaaagcggc accaggtgat gcatatggat
     5221 aaagccttca gcgatatcac catagatctt caccagatca acgccgcata cgttgcactg
     5281 gcatccatga tgttcgaccg ctttatctcg ggccttttta tcgcgttcat agcggtttac
     5341 tgtcaccttt atcgccgcac cttcaaggta tgtctcctgc gacggcaact catccgggaa
     5401 cagaacgtcg gctatgggag ccgcagcggc tttatcctga tgaatatgaa atccctgctc
     5461 tatgagtagt ttgacgcatt tagattttat tccgccggtg aaatccgcag gggtaaattg
     5521 cgtccccgtc atcaatgtcg ctgcgatgcc agcaatcgcc ttcgaggggt aacgtcggcc
     5581 atgatattca atctcataca accgcgcagg ctggaactga tgagtttctc ccgcatccca
     5641 taaatggata gcttcgatga tataccgccg cggaagatga tcagggagtt tattcatgga
     5701 gaatgtcctt atacgttctc agaacaggca gcaaaaaaac aaatgagatc aaggtaatat
     5761 atcttcactt gcaggcttga acagcagaaa atttgcgagc atgatcgcaa ttatttcact
     5821 gccagggata tcaatggctt ccagtaaatc gctacagcag gcaattgcca atataaaaat
     5881 ctggcacaag ggtgaacagc gcgcgccgca taagccattg ttattgctat acgtattagc
     5941 gggatacctg aatggacatc cgcgcctttt cgattattgt agtggaacag tctacgagga
     6001 tgcagttcga agtgagtaag taaaggttgc tggaaacgat caatgccgcc atacaatgat
     6061 ttttgaggat caacaatgag taataaaaac tacgaaagtc accgcaaagc gattgttagt
     6121 aaaggtatac cacccgctct attaaatagg ctcaccaatt cggatgttca ggtgatcaat
     6181 accttcttaa cacgagtgag caaactggaa ctatctcaac aagagaaaga ctggatcata
     6241 aaaatcatct ctatggttta gacaatttca gccttgtagc ctttcaaggg ttttgtgtgg
     6301 gtacaaacat tatcgaatcg ggatatagcc gcagctgtag ctgtatccac atcagtaagt
     6361 tgatgtaggt tgacttaggt tgagcgaaaa atagcgaaaa gccttgtgtg gcgcggattt
     6421 cagtcataaa aaaacgtccg ttgacgtcta ttgatgttcc gatggtgcga aggtcgaagg
     6481 ccggactcgc ctttgcgttt aattcgttga aaaataatgg tttgtgagat tttattttga
     6541 ggatttgccc ccaaaatggg acataaaatt gaatactggg tggaagatca caaagttgcg
     6601 taattatttt ccaggtccga aacgcattca agagcaaggc ctctcccggc actgcgtcag
     6661 tcgggatgct aaatctccca catgtagccc tagtaaatcg cgccagaatg tcaggctagt
     6721 ttgcaaatta ctgacggcga gcgttaagtg gttcagagat tgaagcatag gatgcctatt
     6781 gatggttaat aatttaacca aacactaata gctaataaaa ccccacatgg aaagagattt
     6841 tttgcgggtc ttgttttgta ataaatttct tggcaattac tttatttcat atatatatta
     6901 gcaaataacg atgttagata ttacca
//
LOCUS       DAZGQL010000168         6844 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.169, whole genome shotgun sequence.
ACCESSION   DAZGQL010000168 DAZGQL010000000
VERSION     DAZGQL010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6844
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.169"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(143..820)
                     /gene="tli1"
                     /locus_tag="ACHKIR_004819"
     CDS             complement(143..820)
                     /gene="tli1"
                     /locus_tag="ACHKIR_004819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T6SS immunity phospholipase A1-binding
                     lipoprotein Tli1-EAEC"
                     /protein_id="HFO9992450.1"
                     /translation="MTRDKIIFIAPLLALLTGCNSVSSQYADSDKTEWGYKKLPYDEW
                     KFLFIYPKALPALATQALMVDGEGYKTTYQYLDTTRPSQVSVGRWNDHLGATESYYNK
                     GKALPLMLRFCWDSIIDKKSYETLIFFKKGTWEQMVTPYTDNRWNETYYRSTMIIGLA
                     PEGKVRVWLGDRGNPVVPQLDAKITTVSGDKMKMCKGVTKSDFSYGYDEDIKEFIRDK
                     TYPYGNW"
     gene            complement(840..2522)
                     /gene="tle1"
                     /locus_tag="ACHKIR_004820"
     CDS             complement(840..2522)
                     /gene="tle1"
                     /locus_tag="ACHKIR_004820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T6SS effector phospholipase Tle1-EAEC"
                     /protein_id="HFO9992451.1"
                     /translation="MTKYQGYDVTDATHKTSIHNDWKVVVAKKKPARGVTLTIGIFFD
                     GTGNNRENTASRLMKFNECSAARQGVNQKDAQSCEDFLEEINKNSISNGSYRGYYSNI
                     HWLNSLYVADQTLLESQVEAQIQTYISGIGTAAGEADSVIGMGLGTSILDIFEGVVTK
                     TDEAMERITQALSEFMGFNLNPDFCIAKIQFDVFGFSRGAAAARHFANRVMEQDPAIA
                     RAIAKGLRGDFYDGKPSGEVRFLGLFDTVAAIGGISNFFDINGRSNPGVKLELRPSVV
                     KKVFQITAMNEYRYNFSLNSIKGMWPELALPGAHSDIGGGYNPVGSPLQENESLFLSC
                     PEFEIVSDDTRETDTRVYRNAEQARKMLMTLPALKHILPHGKLTTKIRSVGVNNSNQR
                     RAGVIQKQVGAAVFFERMAVPNDWANVCLRVMLDAAQEAGVLFEPIRQTNTELQLPSE
                     LIFLADKAIAQGKAVRLGQEPQAFTEEELYIIGKYTHCSANWNIESDGNLWVDPTTGE
                     IFIHRFGPKGNKAFVFPNKPNDRWIRSVWYMDDQQRLNDNAVKNTKVMMSGV"
     gene            complement(2519..5038)
                     /locus_tag="ACHKIR_004821"
     CDS             complement(2519..5038)
                     /locus_tag="ACHKIR_004821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HFO9992452.1"
                     /translation="MSLTDTLSKTISASLNRYHLIIPSCPSPLDVEHFSGREEMSQTY
                     YYIINFTSTDNDLDAGLLLRKSATFTMGTGGLKEQVSQKVVHGVITDFRRISGSADQA
                     KYQVILEPFIKLLDRQHRSHRFFVNKSVPEVVTEVLQEHGLKGWEFEFRLKKTYPKRE
                     QINQYQESDLQFIQRLLAEVGIFYFFTLQPDTQTEVVHFGDSQAALTFDKTLAINSPS
                     GMNDNRADSVWGLNVTHNVAEASVTTKAYNHREAQYLLQSAPADMTHGDGDGINYGEV
                     YHYHFRHLERGDKIDPVPETANFYARLGHERYLAEQVRITGNSTDATLAPAQVLTITD
                     SLPPTLPALLRNPVLLTCVGFSASRKDALQVVLKGVPYSEVICWRPPLLPRPKVTGTM
                     TARVTSAKEGDIYAWQDASGMYRVKFDADRDDKNPGQESMPVRLAKPYSGDAYGFHFP
                     LIQGTEVAIAFEEGDPDRPYIAHALHDSRHVDHVTDKNGTRNVIRTPANNKLRMEDKR
                     GEEHIKISTEFGGKTQLNLGHNVNASRKLRGEGAELRTDDWISIRGGKGIFISADMQP
                     QAQGKMLDMNEAIRQLEQALSLARSMAKAATAANATQGDISCQQRLNSSLTDLTAPGM
                     LLHAPDGIGMVSARALRIASGSESVGIMSGDNTDISAGQSFTVVAEGAVSLLSRNQGM
                     QLLAAKGRVNIQAQSDDLSMSSQQNLDIQSSEGKVTVSANQELILACGGAYIKLSGGN
                     IELGCPGQILLKSTNMQKMGPTSLDIASVEMPRGFGGGFILTDEAGVPQPSTPYRLTT
                     AEGDILQGITDENGKTAPVNTSIPSVVKVEFGKVKIHGETE"
     gene            complement(5117..5344)
                     /locus_tag="ACHKIR_004822"
     CDS             complement(5117..5344)
                     /locus_tag="ACHKIR_004822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992453.1"
                     /translation="MKIKRTLLIMLSRFIRGAGMGLGASGIVFTVWFFFLSSAEFKYL
                     WGGLSVALFFVGYLMYRFAYAYIYDEWDDYQ"
     gene            complement(5341..5982)
                     /locus_tag="ACHKIR_004823"
     CDS             complement(5341..5982)
                     /locus_tag="ACHKIR_004823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992454.1"
                     /translation="MDSAKEIARDMLEAVNINGDGFLGAVGKGLISLPVSLGYMGYDF
                     IDTRHRRENQNDKFRMARLVEKGIFSRDIIGKIISICLEDFVSRINMEKTGSIVKNVS
                     GDLAGRMAFAELTGVKLGEVLVSRGLAAFFAGSFAGLLLSVGAETSRAIYTSRFLKDR
                     NPVLHDKLKAQGDLDLLYFLVEDIVRPFERACIVADRNSAEFDRICEYFFGGL"
     gene            complement(5969..>6844)
                     /locus_tag="ACHKIR_004824"
     CDS             complement(5969..>6844)
                     /locus_tag="ACHKIR_004824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000431134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO9992455.1"
                     /translation="TGLTSGNGPQGVFLLVGPSGVGKTETALALADVMYGGEKSLITI
                     NLSEYQEPHTVSQLKGSPPGYVGYGQGGILTEAVRKRPYSVVLLDEVEKAHRDVLNLF
                     YQMFDRGFMRDGEGREIDFRNTVILMTSNLGSDLLMQQLSEKPETTESELHELIRPLL
                     RDHFQPALLARFQTVIYRPLTQSAMRIIVEMKLAQVCERLHCHYGLSTSVDERVYDAL
                     TSACLLPDTGARNVESLLNQQLLPVLSRQLLSYLAARQHPTYLSLTWNDEDGIVIAFD
                     GINSDYPQESIYGQC"
BASE COUNT         1796 a         1829 c         1355 g         1864 t
ORIGIN      
        1 tattgtcatc ctgtagcgtt aaacaccaga catcataact ttagtatttt ttactgcgtt
       61 atcattcagc tattgaccgt ttttttacgc cagatgaaaa ttagactttc tttttacata
      121 ctcatcatta ttctggctta agtcaccagt taccgtaagg ataagtttta tctctgataa
      181 actccttaat atcttcatca tatccatatg agaaatcact tttagtaact cccttacaca
      241 tcttcatttt atcacccgat actgtagtaa ttttagcatc caactgaggt accaccggat
      301 taccgcgatc tcccagccag actcttacct tcccttcagg tgccagacca ataatcatcg
      361 ttgaacgata ataggtttcg ttccagcggt tgtctgtgta aggcgtaacc atctgctccc
      421 aggtcccttt tttaaaaaaa atcagagttt cataactctt tttatcaatt attgaatccc
      481 agcaaaaacg taacatcaga ggtaatgctt tccccttgtt ataatacgac tcagtcgcgc
      541 ccagatggtc attccagcgc cctacactga cctgactcgg tcttgtggta tcaagatact
      601 gataagtcgt cttataacct tccccgtcca ccatcaatgc ctgagtggcc agcgcaggca
      661 gagcctttgg ataaataaag agaaatttcc attcatcata gggtaatttt ttataacccc
      721 attcagtctt atccgaatca gcatactgtg agctaaccga attacaccca gttaacaatg
      781 ccagtaatgg tgcgataaaa atgattttat ctctggtcat atattgccac cctgtggtgt
      841 taaacaccag acatcataac tttagtattt tttactgcgt tatcattcaa tcgctgctga
      901 tcatccatgt accatacaga acgaatccag cgatcatttg gtttattcgg aaaaacgaac
      961 gccttattac ctttaggacc aaagcgatgg ataaaaatct ctcccgttgt aggatccacc
     1021 cataaattac catcagactc aatattccag ttggcagaac aatgggtgta ttttccgata
     1081 atatacaact cttcctcagt aaaagcctga ggctcttgcc ccaaacgtac cgcttttccc
     1141 tgagcaatgg ctttgtcagc aagaaatatt agttcagatg gaagttgtaa ctcagtattt
     1201 gtttgtcgaa ttggttcaaa caatacccct gcttcctgag cagcatccag catcacacgc
     1261 aggcaaacat tagcccagtc attaggtact gccatacgct caaaaaatac tgcggctccc
     1321 acctgctttt gtatcacacc agcccgacgt tgattgctgt tgttcacacc tacggaacga
     1381 attttggtgg ttagttttcc atgaggcaaa atatgtttta aggctggcag agtcatcaac
     1441 atttttctgg cttgctctgc attacggtat actcgcgtat ccgtttctct ggtatcgtca
     1501 ctgacaattt caaattccgg gcaactcaga aacagacttt cattttcctg cagaggtgaa
     1561 cctaccggat tgtaaccacc tccaatatct gaatgcgccc ctggtaaagc cagttcgggc
     1621 cacatacctt taatactgtt caggctgaaa ttatatcggt attcattcat tgcagtaatc
     1681 tggaaaactt tcttcacgac tgaaggacgc aattccagct ttactcccgg gttgctacgt
     1741 ccattaatat cgaagaaatt actgattcca ccaatagctg cgacagtatc aaaaagcccc
     1801 agaaaacgga cttcaccaga aggtttacca tcataaaaat cgcctctgag ccctttagca
     1861 atagctctgg ctatagcagg atcctgttcc ataacccgat tggcaaagtg ccttgccgcc
     1921 gctgctcccc ggctaaaacc aaaaacatca aactggattt ttgcgataca aaaatcagga
     1981 tttaaattga accccataaa ttctgataac gcctgggtga ttcgttccat tgcctcgtcg
     2041 gttttcgtca ccacaccttc aaatatatcc agaatactgg tgcctaatcc catgccaatg
     2101 actgaatctg cttcaccagc cgcagtccct atcccggaaa tgtaagtctg aatttgtgct
     2161 tcaacctgac tttccaacaa agtctgatct gctacgtaaa gtgaatttag ccagtgaata
     2221 ttggaataat atcctcgata actgccattc gagatactgt tcttgttaat ttcttcaaga
     2281 aagtcctcac agctttgtgc atctttttga tttaccccct gtctggctgc actacactca
     2341 ttaaatttca tcaggcgaga tgctgtattc tcccgattgt ttccggtacc atcgaaaaat
     2401 ataccaatgg ttaaagtcac accccgcgcc ggttttttct ttgcaacaac aactttccag
     2461 tcattatgaa tactggtttt atgggtggca tctgttacgt catatccctg atattttgtc
     2521 attctgtttc tccatgaatt tttaccttcc caaactcaac ttttactact gacggaatgg
     2581 aggtgttgac tggcgctgtt ttaccatttt catcggtgat cccttgtaga atatcacctt
     2641 cagccgttgt taatcgataa ggcgtcgaag gttgcggcac tcccgcttca tcggtaagaa
     2701 taaaaccgcc accaaaccct cgcggcattt caacagacgc aatatcaaga cttgttggtc
     2761 ccatcttctg catattcgtg ctttttaaca ggatctgtcc cgggcatccc agctcaatat
     2821 ttccaccgct tagtttgata taagctccac cacatgccag tatcagttcc tgattagcag
     2881 aaacagtcac tttgccctca gaactttgaa tatcaagatt ctgttgggaa ctcattgaca
     2941 aatcgtcact ttgagcctga atatttaccc gcccttttgc tgctaacagt tgcatcccct
     3001 ggtttctgct caacaggcta acagcacctt ctgctacaac tgtaaatgac tgcccggcac
     3061 taatatcagt gttatctccc gacattatgc ccacgctttc actacccgaa gcaattcgta
     3121 acgcccgtgc actaaccatg ccaatcccat caggagcatg caacagcatt cccggcgcag
     3181 tgagatctgt tagcgatgaa ttcaaccgtt gctgacaact tatatctccc tgagtagcat
     3241 ttgcagcagt tgcggccttc gccatacttc gggccagaga taatgcctgc tccagttggc
     3301 ggatagcttc attcatatcc agcatctttc cttgtgcctg aggctgcata tctgcactga
     3361 taaatattcc ttttcctccc cggatactta tccagtcatc agtcctcagc tcagcgcctt
     3421 ctcccctgag tttccgggag gcattaacgt tatgaccaag attaagttgt gtcttaccgc
     3481 caaactcggt actgatcttg atatgctcct caccgcgttt gtcctccatc cgcaacttgt
     3541 tatttgccgg cgtacggata acattgcgcg taccgttctt atcggtaacg tgatcaacat
     3601 gacgtgaatc atgcagggca tgcgcgatat aggggcgatc cggatcacct tcctcaaagg
     3661 caatggccac ttccgttccc tgaatcagcg gaaaatgaaa accataagca tcaccactgt
     3721 agggtttggc cagacgtacc ggcatgcttt cctgtcccgg atttttatca tcacggtcag
     3781 catcaaattt cacccggtac atacctgatg catcctgcca ggcgtagata tccccttctt
     3841 ttgcactggt cacacgggcc gtcatggtac cggtaacttt cggacgcggc agtaaaggag
     3901 ggcgccagca gataacttca ctgtaaggaa cgcccttcag tacaacctgc agggcatcct
     3961 tgcgacttgc agaaaaaccg acacaggtaa gcaacaccgg attgcgcagc agcgcaggca
     4021 acgtcggcgg aagactgtcg gtgatagtca gaacctgagc aggtgccagc gtggcgtctg
     4081 tactgttgcc ggttatccgc acctgctccg ccagataacg ttcatgacca aggcgggcat
     4141 aaaaattggc cgtctccggc accgggtcga ttttatcccc ccgttccaga tgacggaaat
     4201 ggtagtggta cacctcacca tagttaattc cgtcaccatc accatgcgtc atgtctgccg
     4261 gggcggactg caacagatat tgtgcttcac gatggttata agcctttgtg gtgacactgg
     4321 cctctgccac attgtgcgtc acattcagcc cccacactga atccgctctg ttatcattca
     4381 ttccggacgg actgtttatc gccagcgtct tatcaaacgt cagcgcagcc tggctgtcac
     4441 caaaatgcac tacttccgtc tgcgtgtccg gttgcagggt gaaaaaataa aaaatcccca
     4501 cttcagccag caaacgctga ataaattgca ggtcactttc ctgatactgg ttaatctgtt
     4561 cacgcttcgg atacgttttt ttgagacgga actcaaactc ccagcctttc aggccgtgct
     4621 cctggagaac ctctgtcacc acctccggca ccgatttgtt aacgaaaaat cggtgagaac
     4681 gatgctgcct gtccagtagt ttaataaatg gttccagtat cacctggtat tttgcctggt
     4741 cggcggagcc cgatatacgg cgaaaatctg taatgacacc atgtactact ttctggctca
     4801 cctgctcctt aagccctccc gtacccattg taaatgtggc tgatttacgc agcagcagcc
     4861 cggcatccag gtcgttgtca gtactggtaa aattgatgat gtaataatag gtctgactca
     4921 tctcctccct gccgctgaaa tgctcaacat ccagtggtga cgggcaggag ggaataatta
     4981 aatggtaacg attcagggaa gcagagatag tttttgaaag tgtatctgtc agactcatat
     5041 tgttaactcc atgtctgtac cggtattgaa ctgcaattat ataatgatga ccgttaagct
     5101 ttattttcag agagaattac tgataatcat cccattcatc gtaaatatat gcatatgcaa
     5161 atcgatacat cagataaccg acaaaaaata acgcaacaga gagccctccc cacagatatt
     5221 taaattcagc agatgagaga aaaaagaacc agactgtaaa tacaataccc gacgcaccta
     5281 accccatacc tgcgccacgg ataaatcggg aaagcattat aagtaatgtt ctttttatct
     5341 tcataaacca ccgaaaaaat attcgcatat ccggtcaaac tcagccgaat ttcggtctgc
     5401 gacaatacag gctcgttcaa acgggcgcac aatatcttca acaagaaagt acaaaagatc
     5461 caaatccccc tgagctttca gcttatcatg caacaccgga tttctgtctt tcagaaaacg
     5521 ggatgtgtaa atcgcacgcg atgtctctgc gccaacagat aataacaacc cggcaaaact
     5581 ccctgcaaaa aaagcagcca gccctctgga taccagaacc tctcccagtt taaccccggt
     5641 caattcagca aatgccattc tacctgccag atctcctgaa acgttcttca caatggagcc
     5701 tgttttttcc atattgatcc gggatacaaa gtcttcaaga caaatactga taattttccc
     5761 aataatatca cggctgaaaa tgcctttttc taccagtctg gccattctga atttgtcatt
     5821 ctggttttcc cgcctatgcc tggtatctat aaaatcatat cccatataac cgagactgac
     5881 tggcaaagat atgagtccct tcccgacggc ccccagaaac ccatccccat taatatttac
     5941 ggcctccagc atatcccggg caatttcttt agcactgtcc atatatgctt tcctgcggat
     6001 aatcgctatt gattccatca aacgctatca caataccatc ctcatcattc cacgtcagag
     6061 acaggtacgt cggatgctgt ctggcagcaa gatagctgag caactgccgg ctcagtaccg
     6121 gcagcagttg ctggttcagc agactctcca cattccgggc acccgtatcc ggcagcaggc
     6181 aggcagatgt cagggcatcg tacacacgtt catcaaccga tgtgctcagt ccataatggc
     6241 agtgcaggcg ttcacagacc tgtgcaagct tcatctccac aatgatgcgc atggcagact
     6301 gcgtcagcgg acggtaaatc acggtctgga aacgggcaag cagtgctggc tggaagtggt
     6361 cgcgcagcag tggacggata agctcatgca gctccgattc cgttgtctcc ggcttttcgc
     6421 tcagttgctg catcagaagg tcactgccca gattggaggt catcagaatg accgtattac
     6481 ggaagtcaat ttcacgcccc tcaccgtcgc gcataaagcc ccggtcaaac atctggtaga
     6541 acagattcag cacatcccgg tgggcctttt ccacttcatc cagcagcacc acgctgtaag
     6601 ggcgtttacg cacagcctcc gtcagaatac cgccctgacc gtatcccacg tacccgggcg
     6661 gtgacccctt aagctgggaa accgtatggg gctcctggta ttccgacaga ttgatggtaa
     6721 tcagtgattt ttcgccgccg tacatcacat ctgccagcgc cagtgccgtt tcggtttttc
     6781 ccacaccact gggaccgacc agcaggaaca cgccctgggg gccgttaccg gatgtcagac
     6841 ccgt
//
LOCUS       DAZGQL010000169         6613 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.170, whole genome shotgun sequence.
ACCESSION   DAZGQL010000169 DAZGQL010000000
VERSION     DAZGQL010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6613
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.170"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..3649)
                     /locus_tag="ACHKIR_004825"
     CDS             complement(<1..3649)
                     /locus_tag="ACHKIR_004825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HFO9992456.1"
                     /translation="MSEGPGGPQGATAGGTLAMRMLSQQAMVASQMKRAANDKAIAQM
                     LAAKKSGPPAARLGDEIQHKSFLGALAGAVLGAIVTIAEGCLIMAACATGPYALVLVP
                     ALMYASYKASDYVEEKQNQLESWINSFCDTDGAINTGSENVKINGKPAARAAVTLPPP
                     PLTEAIPSPPQGEPSWGDIATDLLESAAEKAVPLAKAWGNAVITLTDSNAGIMDRVSA
                     GASLLFPAGPVLMEFATMVGGRGEIKKDVDFPEAGEDTALCDKENKPPRIAQGSSNVF
                     INNQPAARKGDKLECSAAIVEGSPDVFIGGEQVTYLDIQPEFPPWQRMILGGITIASY
                     LLPPAGLLGKLGNLAKLGKLGNLLGKSGKLLGAKLGALLGKTGKSLKSIANKVIRWVT
                     DPVDPVTGAYCDERTDFTLGQTLPLSFTRFHSSVLPLHGLTGVGWSDSWSEYAWVREQ
                     GNRVDIISQGATLYFAFDGDSDTAVNPYHAQYILRRRDDYLELFDRDALSSRFFYDAF
                     PGMRLRHPVTDDTNDDRLAHSPGDRMYMLGGMSDTASNRITFERDSQYRITGVSHTDG
                     IRLKLTYHASGYLKAIHRTDNGIQTLATYEQDARGRLTEADARLDYHLFYEYDAADRI
                     IRWSDNDQTWSRFTYDEQGRCVNVTGADGYYNATLDYSDGCTTVTDGKGIHRYYYDPD
                     GNILREEAPDGSTTTYEWDEFHHLLARHSPAGRVEKFEYNAVHGQLSRYTAADGAVWL
                     YRYDERGLLSNITDPAGQTWTQQCDERGLPVSLVSPQGEETRLAYTAQGLLSGIFRQD
                     ERRLGIEYDHHNRPETLTDVMGREHHTEYSGHDLPVKMRGPGGQSVRLQWQQHHKLSG
                     LERAGTGAEGFRYDRHGNLLAWTDGNGVDWTMEYGPFDLPVARTDGEGHRWQYRYDKD
                     TLQLTEVINPQGESYRYLLDNCGRVTEEHDWGGVTWRYRYDADGLCTARVNGLEETIL
                     YSRDAAGRLAEIITPEGKTQYAYDKSGRLTGIFSPDGTSQRTGYDERGRVNVTTQGRR
                     AIEYHYPDEHTVIRCILPPEDERDRHPDESLLKTTYRYNAAGELTEVILPGDETLTFS
                     RDEAGREVLRHSNRGFACEQGWNAAGQPVSQRAGFFPEEATWGGLVPSLVREYRYDSA
                     GNVSGVTSREDYGRETRREYRLDRNGQVTAVTASGTGLGYGEGDETYGYDSCGYLKAQ
                     SAGRHRISEETE"
     gene            complement(3669..4130)
                     /locus_tag="ACHKIR_004826"
     CDS             complement(3669..4130)
                     /locus_tag="ACHKIR_004826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HFO9992457.1"
                     /translation="MQFTFNEGHIQLPSQWQDQSMQVLVSTDNSGINLVITREAVPQG
                     TLTPELYQETLALYQGKLDGYTEHACREITLAEAPAWLLDYSWNGPEDEGNQGRIDQI
                     AVFQRRGDTLLTFTFSTSLSLKNSQKTMLLEVIKSFTPLPPENDIQKDQPR"
     gene            complement(4158..6059)
                     /locus_tag="ACHKIR_004827"
     CDS             complement(4158..6059)
                     /locus_tag="ACHKIR_004827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HFO9992458.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLTQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTVWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVRPPLWRA
                     GLRQNFRIFQNEDIRSILGTILQENDVTEWSPLFSEPHPSREFCVQYGETDYDFLCRI
                     AAEEGIFFYEEHAQKSTDQSLVLCDTVRYLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFDQEGQHQDYQRTQYEVYDWPGRFKSAHGQNFARWQ
                     MDGWRNNAETARGMSRSPEIWPGRRIVLTEHPQANLNREWQVVASELHGEQPQAVPGR
                     RGSGTTLNNHFAVIPADRTWRPQPKPKPSVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPANQDSSCWIRVAQAWAGPGFGNLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HEDNRSPGSLPGTKTQMTIRSKTYMGSGFNELKFDDATGKEQVYIHAQKNMDTEVLND
                     RTTTVKHDHRETVKNDQTVMIQEGNRLLTVEKGHKITGVLKGSLSEDVFQDRGTIAGS
                     VHVDAVNNGGEGDGIQAYTAIKQIMLAVEESKIALTPDGIQLQVGESTVIRLSKDGIT
                     IVGGSVFIN"
BASE COUNT         1268 a         1956 c         1792 g         1597 t
ORIGIN      
        1 gctcagtctc ttcacttatc cggtgcctgc ccgcagactg cgccttcagg tagccgcagc
       61 tgtcatagcc ataagtctca tcgccttcgc catagcccag cccggtgcct gaggctgtca
      121 ccgccgtgac ctggccgttc cggtccagcc ggtactcccg gcgtgtttcc cgtccgtaat
      181 cttcccggct ggtgacacct gacacgttac ccgcgctgtc gtaacggtac tcccgtacca
      241 gtgacggaac cagcccgccc catgtggctt cttccggaaa aaatccggcg cgctggctga
      301 caggctggcc ggctgcattc cagccctgct cacaggcaaa accccggtta ctgtgccgga
      361 gcacttcacg tcctgcctca tcacggctga acgtcagcgt ctcatccccc ggcaggataa
      421 cctccgtcag ttctccggcg gcgttgtagc ggtaggtggt tttcagcagg gattcatcgg
      481 ggtgtctgtc gcgttcatct tccggtggca ggatacagcg gatgacggtg tgttcatcgg
      541 ggtaatggta ctcaatggcc cgtcggccct gagtggtgac attcacccgc ccgcgttcgt
      601 catagccggt gcgctgtgat gtgccgtccg ggctgaagat acccgtcagc ctgccggatt
      661 tgtcatacgc atactgcgtt ttgccttccg gcgtgatgat ttctgccagg cggcctgcgg
      721 cgtcccggct gtagaggatg gtttcctcca ggccgttaac cctggcggta cacaggccat
      781 cggcgtcata gcggtaacgc caggtcacgc cgccccagtc atgctcttcc gtcacccgcc
      841 cacagttgtc gagaagataa cggtatgact caccctgcgg gttgatgact tctgtcagtt
      901 gcagcgtgtc tttatcatag cggtactgcc agcggtggcc ttcaccgtcc gttcgcgcca
      961 ccggcaaatc aaacgggccg tattccattg tccagtccac accgttaccg tccgtccagg
     1021 ccagcaggtt gccgtggcgg tcatagcgga atccttctgc gccggttcct gcccgctcaa
     1081 ggccgctcag tttatggtgc tgctgccact gcaaccgcac tgactgaccg ccggggccgc
     1141 gcatcttcac cggcaggtcg tgaccactgt attcggtgtg gtgttcacgg cccatcacgt
     1201 cggtgagtgt ttccggccgg ttgtggtggt cgtactctat gcccagacgc cgttcatcct
     1261 ggcggaatat ccccgacagc agcccctgag cggtgtacgc cagccgggtc tcttcgccct
     1321 gtggcgatac cagactcacc ggcaggccgc gttcatcgca ctgctgtgtc cacgtctgcc
     1381 cggcagggtc ggtgatgttg ctgagcaggc cgcgctcatc atagcggtac aaccacaccg
     1441 cgccgtctgc cgccgtataa cggcttaact gaccgtgtac ggcgttgtat tcaaatttct
     1501 ccacccgtcc ggcaggggag tggcgggcca gcaggtgatg gaattcatcc cattcatacg
     1561 tggtggtgct gccgtccggc gcttcttccc gcagaatatt gccgtcagga tcatagtaat
     1621 aacggtgaat gcccttgccg tccgtcacgg tggtgcagcc gtcactatag tccagcgtgg
     1681 cgttgtaata gccgtccgcc ccggtgacat tcacgcaccg gccctgttca tcgtaggtga
     1741 aacggctcca cgtctggtcg ttatcggacc agcggatgat ccggtccgca gcgtcgtact
     1801 cataaaacag gtggtagtcc agccgcgcat ccgcttcggt cagccgcccc cgcgcatcct
     1861 gttcgtaggt cgccagcgtc tgtatgccgt tatccgtgcg gtgaatggct ttcaggtagc
     1921 cgctggcgtg gtacgtcagt ttaagccgga tcccgtcggt gtgactgact cccgtgatgc
     1981 ggtactggct gtcgcgctca aacgtgatgc ggttgctggc ggtgtcgctc atcccgccca
     2041 gcatgtacat ccggtcaccg gggctgtgtg ccaggcggtc atcattggta tcgtcagtca
     2101 ccgggtggcg caggcgcatt cccggaaagg cgtcataaaa gaagcggctg ctcagggcat
     2161 cacggtcgaa cagctccagg taatcgtcgc ggcggcgcag gatatactgg gcgtgatacg
     2221 ggttaaccgc cgtatcactg tctccgtcga aggcaaagta cagcgtggct ccctggctga
     2281 tgatatccac ccggtttccc tgttcacgca cccaggcgta ttcgctccag gagtcgctcc
     2341 agcccacgcc cgtcaggcca tgcagtggca gtacagaact gtggaaacgg gtgaaggaga
     2401 gggggagtgt ctggcccagc gtaaagtcgg tacgttcgtc gcagtatgcg ccggttaccg
     2461 gatcgacagg atctgttacc catctgatga ctttattggc aatacttttt aacgacttac
     2521 ctgttttccc cagcaacgcg ccgagctttg cccccagcag cttcccgctt ttccccagca
     2581 ggtttcccag tttgccgagt ttcgccagat tccccagttt tcccagcagt cccgcgggcg
     2641 gcagaagata gctggctatc gttattcccc ccaggatcat tctctgccac ggcgggaact
     2701 ccggctggat atccagatag gtcacctgtt caccgccaat aaagacgtcc ggcgaacctt
     2761 ccacgattgc cgcgctgcac tccagtttgt cgcccttgcg cgcggcaggc tgattgttga
     2821 taaagacgtt gctgctgccc tgggctatcc tcggtggttt gttctccttg tcgcagagcg
     2881 ccgtgtcctc accggcttcc gggaaatcca cgtctttttt gatttcgcca cgcccgccca
     2941 ccatggtggc aaactccatt aataccggac cggcgggaaa cagaagcgat gcgccggcgc
     3001 tgacgcgatc cataataccg gcattgctgt ccgtcagggt gataacagcg ttcccccagg
     3061 ccttcgccag tggcactgct ttttccgctg ccgattcaag caggtcagtg gcaatatccc
     3121 cccatgaagg ttccccctgt ggtggagagg gtattgcttc agtcagggga ggagggggaa
     3181 gggtgacggc ggccctggct gctggcttac cgttaatttt tacgttttca gaaccggtat
     3241 tgatggcgcc gtccgtgtca caaaaactgt ttatccatga ctcaagctgg ttctgtttct
     3301 cctccacata atcactcgcc ttatagctgg catacatcag cgcaggcacc agaaccagcg
     3361 cataagggcc ggtggcgcag gcggccataa tcaggcaacc ttctgcgata gttactatcg
     3421 cccccagcac ggcccctgcc agtgccccca gaaaactttt atgctgaatt tcatcgccca
     3481 gcctggcggc aggtgggccg gacttctttg ctgccagcat ctgtgcaatg gctttgtcgt
     3541 tggctgcccg tttcatctgg ctggcgacca tcgcctgctg tgacagcatt cgcattgcca
     3601 gcgtaccgcc tgcggtcgct ccctgtgggc cgcctggtcc ttcactcatg tgttatccct
     3661 cgtttatgtc agcgcggctg gtccttttgg atgtcattct ccggtggcaa cggggtgaag
     3721 ctctttatca cctccagcag catcgtcttt tgactgttct tcagggataa ggaggtgctg
     3781 aaagtgaagg tcagcagcgt atctccacgc cgctggaata cggctatctg gtcaatccgt
     3841 ccctggttcc cctcatcttc cggaccgttc caggaataat ccagcagcca ggccggggcc
     3901 tctgccagag tgatttcccg gcaggcatgt tcggtgtacc catcaagttt gccctggtac
     3961 agcgccagcg tttcctggta cagctctggt gtcagggttc cctgtggaac ggcttcgcgg
     4021 gtaatgacca ggttgatgcc actgttatct gtggacacca ggacctgcat tgactgatcc
     4081 tgccattgcg atggcagctg aatatgacct tcattaaaag tgaactgcat aaaatactcc
     4141 ctgataatct gaatgaatca gttgatgaaa acagaaccgc ccacgatggt gatgccatcc
     4201 ttcgacagcc tgattaccgt cgattccccg acctgtagct gaatgccatc cggcgtcagc
     4261 gcaattttgc tttcctccac ggccagcata atttgcttaa ttgccgtata agcctgtata
     4321 ccgtcgcctt cgccaccatt gtttacagcg tcaacatgca ctgaaccggc aatcgtgcct
     4381 ctgtcctgaa aaacatcctc agataaagac ccttttagta cgcctgtgat tttgtggcct
     4441 ttttccaccg taagaaggcg gttaccttcc tggatcatga ccgtctggtc attttttacg
     4501 gtttcgcggt gatcgtgttt tacggtggtg gtacggtcgt tgagtacctc ggtatccatg
     4561 ttcttctggg cgtggatgta gacctgttct ttccctgtcg catcgtcaaa cttcagctcg
     4621 ttaaacccgc tgcccatata tgttttggag cggatggtca tctgcgtctt cgttcccggc
     4681 aggctgcccg gcgagcggtt atcttcgtgg taggtgcgcc ccataatgat cggctggtcc
     4741 ggatcgccgt tgaggaagtc cacaatcacc tcctgaccca cgcgcggtat cgccaggttg
     4801 ccaaatcccg gccccgccca cgcctgcgcc acccgtatcc agcacgaact gtcctgattg
     4861 gccgggttat agcggtccca gttgaatttc acccgcaccc ggccatgctc gtcgcagaag
     4921 atttcctctc ccgcaggccc cgtcacgacg gcgctctgcg gaccgtccac tgacggcttt
     4981 ggcttcggct gaggtcgcca cgtccggtct gccggtatta ccgcaaagtg gttattcagc
     5041 gtggtacctg accccctgcg tcccggcacc gcctgcggct gttcgccgtg cagttcgctc
     5101 gccaccacct gccattcccg gttcaggttc gcctgcggat gctccgtcag cacaattcgc
     5161 cgtccaggcc atatctccgg cgagcggctc attccccgcg cggtttcggc gttgtttcgc
     5221 cagccgtcca tctgccagcg ggcaaagttc tgcccgtggg cgctcttgaa acgcccgggc
     5281 cagtcataca cttcatactg tgtgcgctgg taatcctgat gctggccttc ctggtcaaaa
     5341 cgtccggccc agccggggcg tttaaaggtg tagtctttgg tcaccacaga ggaaggacgg
     5401 atttgtgcgc tgtagcggaa ctggctgatg cagagggtgc tcacctcggt acgggtgttc
     5461 gggttccagg ggatttcaaa ggattccggc agatagcgga ccgtgtcgca cagcaccaga
     5521 ctctggtcgg tacttttctg cgcgtgctcc tcatagaaga agatgccttc ctccgctgct
     5581 atccggcaga gaaagtcgta atcggtttca ccgtactgga cacaaaactc acgggaagga
     5641 tgcggctcac tgaacagcgg gctccactcc gtgacgtcat tttcctgcag tatggtgccg
     5701 aggatgcttc tgatgtcctc gttctggaat atacggaagt tctgacgcag tcctgcccgc
     5761 cacagcggcg ggcgcacctt catgctgtac agcatctggt ttttgtcgtt ctccccgagt
     5821 tcaaaccagg tcaccacgcc tttcacccgg cgctgtacgt catcgccctg ccataccgtc
     5881 aggtaggcca ttttgtccag cacctgcgca aattcaaggg agagaaactg ctgactgacc
     5941 agagaaaggt caagggaaaa aagtgaagag agtgactggg tgagatgaaa ggagaccacc
     6001 gcaaaagcgt ccggtggcag gccgtccact tccagtgtga aacgtaatcc ggttgacata
     6061 gcaacctccg tgaaaatgaa taataatccg catatattgc atgtacaaca tgtcgggtaa
     6121 tgagataagc gggccggctt caggcgttac cggggaatat cagaaaagtg tcatggttat
     6181 gtgatgattt tgtccgggat tttacttcag agaaacgggg ggctgttgac tcaacaaaat
     6241 aatcacaaac tcaactgacg ggttcacgat gttattaagg acaggctcgt atattacatc
     6301 gagtaacaaa aaaacacctg atatgcaaag tcaaaccaat atttgtaatg tctttttatt
     6361 cacaataata aataactcaa taaacattat tgaaatgaat gatggtttgt ataaggaata
     6421 tacgctaccg ccccaccctg ataacatgat ctgcacattg ctatcttaac gtcgttagac
     6481 gctggcttaa tagtgaatat tttaaattag gaatttatca agttcacttg ataaatagac
     6541 cgcgtgaata atatagagat taatgatgcc tgactgcgct ttatattata gccgattctt
     6601 ttatagtgat gaa
//
LOCUS       DAZGQL010000170         6531 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.171, whole genome shotgun sequence.
ACCESSION   DAZGQL010000170 DAZGQL010000000
VERSION     DAZGQL010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6531
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.171"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            171..821
                     /gene="csgD"
                     /locus_tag="ACHKIR_004828"
     CDS             171..821
                     /gene="csgD"
                     /locus_tag="ACHKIR_004828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm master transcriptional regulator CsgD"
                     /protein_id="HFO9992459.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            826..1215
                     /gene="csgE"
                     /locus_tag="ACHKIR_004829"
     CDS             826..1215
                     /gene="csgE"
                     /locus_tag="ACHKIR_004829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HFO9992460.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            1240..1656
                     /gene="csgF"
                     /locus_tag="ACHKIR_004830"
     CDS             1240..1656
                     /gene="csgF"
                     /locus_tag="ACHKIR_004830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HFO9992461.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            1683..2516
                     /gene="csgG"
                     /locus_tag="ACHKIR_004831"
     CDS             1683..2516
                     /gene="csgG"
                     /locus_tag="ACHKIR_004831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HFO9992462.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQGLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(2580..3071)
                     /gene="ycdZ"
                     /locus_tag="ACHKIR_004832"
     CDS             complement(2580..3071)
                     /gene="ycdZ"
                     /locus_tag="ACHKIR_004832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFO9992463.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYAITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQQIK
                     NKA"
     gene            complement(3173..3727)
                     /gene="ycdY"
                     /locus_tag="ACHKIR_004833"
     CDS             complement(3173..3727)
                     /gene="ycdY"
                     /locus_tag="ACHKIR_004833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HFO9992464.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEGATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(3751..4488)
                     /gene="ycdX"
                     /locus_tag="ACHKIR_004834"
     CDS             complement(3751..4488)
                     /gene="ycdX"
                     /locus_tag="ACHKIR_004834"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HFO9992465.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(4543..5481)
                     /gene="ghrA"
                     /locus_tag="ACHKIR_004835"
     CDS             complement(4543..5481)
                     /gene="ghrA"
                     /locus_tag="ACHKIR_004835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016618 - hydroxypyruvate reductase
                     [NAD(P)H] activity [Evidence IEA];
                     GO_function: GO:0030267 - glyoxylate reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HFO9992466.1"
                     /translation="MDIIFYHPTFDTQWWIEALHKAIPQARVRAWKSGDNESADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLKPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVDYISRTIAQLEKGERVCGQVDRARGY"
     gene            5714..5801
                     /locus_tag="ACHKIR_004836"
     tRNA            5714..5801
                     /locus_tag="ACHKIR_004836"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5748..5750,aa:Ser,seq:gga)
     gene            complement(5951..>6531)
                     /locus_tag="ACHKIR_004837"
     CDS             complement(5951..>6531)
                     /locus_tag="ACHKIR_004837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFO9992467.1"
                     /translation="NLDREIWRDLMQCSGMLSLMDAQARDTWYRSLEYDNFPEISEEN
                     ILSTFEQLHQNKDEVFERGVINVFRGLSWNYKTNCPCKFGNKIIVNNLVRWDQWGLHL
                     ITGQQADRLADLERMLHLFSGKPIPDNRENITIHLDDHIRSLQGKECYEDEMFIIKYF
                     KKGSAHITFRKPELVDRLNDIIAKHYPDMLAV"
BASE COUNT         1746 a         1609 c         1488 g         1688 t
ORIGIN      
        1 tttgtaatgg ctagattgaa atcagatgta atccattagt tttatatttt acccatttag
       61 ggctgattta ttactacaca cagcagtgca acatctgtca gtacttctgg tgcttctatt
      121 ttagaggcag ctgtcaggtg tgcgatcaat aaaaaaagcg gggtttcatc atgtttaatg
      181 aagtccatag tattcatggt catacattat tgttgatcac taaaccttct ttgcaggcga
      241 cagctctctt gcagcacctt aagcaatcgc tggcaattac aggaaaatta cataatattc
      301 aacgttctct ggacgatatc tcttcaggct ctattattct tctggatatg atggaagcgg
      361 ataaaaagct tatccattat tggcaggata ctttgagcag gaaaaacaac aatatcaaaa
      421 tattgttgct aaatacgcct gaagattacc cgtaccgcga cattgaaaac tggcctcata
      481 tcaacggcgt tttttatgcc atggaggatc aagaacgtgt tgtcaatggg ttgcaaggcg
      541 tcctgcgcgg cgaatgctac tttacgcaaa agcttgccag ttacctgatt acgcattcag
      601 gtaactatcg ttataacagc acggaatcag ccctccttac tcatcgggaa aaagagatcc
      661 tgaataagct gcgtatcggc gcgtctaata acgagatcgc tcgttcgttg ttcatcagcg
      721 aaaatacggt taaaacgcat ctttataatc ttttcaagaa gatagccgta aaaaaccgga
      781 cacaagcggt ttcctgggca aacgataacc tcaggcgata aagccatgaa acgttattta
      841 cgctggattg tggcggcaga atttctgttc gccgcaggga atcttcacgc cgttgaggta
      901 gaagtcccgg gattgttaac tgaccatact gtttcatcta ttggccatga tttttaccga
      961 gcctttagtg ataaatggga aagtgactat acgggtaact taacgattaa tgaaaggccc
     1021 agtgcacgat ggggaagctg gatcacaata acggtgaatc aggacgttat tttccagact
     1081 tttttatttc cgttgaaaag agacttcgag aaaactgtcg tctttgcact gattcaaact
     1141 gaagaagcac taaatcgtcg ccagataaac caagcgttat taagtacggg cgatttggcg
     1201 catgatgaat tttaaataat aaattgttcg gaggctgcaa tgcgtgtcaa acatgcagta
     1261 gttctactca tgcttatttc gccattaagt tgggctggaa ccatgacttt ccagttccgt
     1321 aatccaaact ttggtggtaa cccaaataat ggcgcttttt tgttaaatag cgctcaggcc
     1381 caaaactctt ataaagatcc gagctataac gatgactttg gtattgaaac gccctcggcg
     1441 ttagataact ttactcaggc catccagtca caaattttgg gtgggctact gtcgaatatt
     1501 aataccggta aaccgggccg catggtgacc aacgattata ttgttgatat tgctaaccgc
     1561 gatggtcaat tgcagttgaa cgtgacagat cgtaaaaccg gacaaacctc gaccatccag
     1621 gtttcgggtt tacaaaataa ctcaaccgat ttttaagccc cagcttcata aggaaaataa
     1681 tcatgcagcg cttatttctt ttggttgccg tcatgttact gagcggatgc ttaaccgccc
     1741 cgcctaaaga agccgccaga ccgacattaa tgcctcgtgc tcagagctac aaagatttga
     1801 cccatctgcc agcgccgacg ggtaagatct ttgtttcggt atacaacatt caggacgaaa
     1861 ccgggcaatt taaaccctac ccggcaagta acttctccac tgctgttccg caaagcgcca
     1921 cggcaatgct ggtcacggca ttgaaagatt ctcgctggtt tataccgctg gagcgccaag
     1981 gcttacaaaa cctgcttaac gagcgcaaga ttattcgtgc ggcacaagaa aacggcacgg
     2041 ttgccattaa taaccgaatc ccgctgcaat ctttaacggc ggcaaatatc atggttgaag
     2101 gttcaattat cggttatgaa agcaacgtta aatcgggcgg ggttggggca agatattttg
     2161 ggatcggtgc agacacgcaa taccagcttg atcagattgc cgtgaacctg cgcgtcgtca
     2221 atgtgagtac cggcgagatc ctctcctcgg tgaacaccag taagacgata ctttcctatg
     2281 aagttcaggc gggggtcttc cgctttattg actaccagcg cttgctggaa ggggaagtag
     2341 gttacacctc gaacgaaccc gttatgctgt gcctgatgtc ggctatcgaa acaggcgtca
     2401 ttttcctgat taatgatggt atcgaccgcg gtctttggga tttgcaaaat aaagcagaac
     2461 ggcagaatga cattctagtg aaataccgcc atatgtcggt tccaccggaa tcctgataga
     2521 taaaaatgcc ggaattatca tccgacattt tacggtttaa gcgaaagcgt gagtgcccct
     2581 cacgctttat ttttgatttg ctgctcacgg tgagccgtct ttgcactacg cgccgccaac
     2641 cacaggccgc tgtttttcat tgcgtaacca aataccagtc caagcgccag cgaaggtaac
     2701 accagtttcc agtcaccctg cccggcaaac gtcgcgcatg cgcctataaa cgtccccgga
     2761 acaaatgaaa gcagcagctg tttggcctgg atacacatca gaaacgcgac aataccggtt
     2821 atggcataac cgagtatttc cagatgtggt gccagtgcac taccgtaaat aatgaccatc
     2881 gcccacacca cgccactaag cagggttgca gcggagatcg ccagcccttt cagcccacct
     2941 tgtgggcagg caaagtaggc cgtacagccc aggaagcccg cccagcttag taagcccagg
     3001 gaaacagcca cccatcccca gataccggag agaatgcctg ttgtgattgc aatagagaga
     3061 agtatgttca tgccgcgcat gatagcagaa aacgcggcca tgaatgagat cgagcacaca
     3121 ttttatgcaa caacaaatta cccgttgcag ttatgagtga tgcagatcac atttactctt
     3181 cagaatcttc ttccagctcg tcccacattg cactaatagc atcgcgggtt agcggtgcca
     3241 tggtgcgcca gaaaggcgta gttgcatggg cctccacttt gccaaggaac gcaccacacc
     3301 agggtaacag atactcactg aacagtgttt ccagtgcttc gctctcatct tccgttgact
     3361 gatcttccag ccaggaagcc gcaagcagca atgtgccgat gtgatcggct ggcgtatccg
     3421 ctagtggcat ccctcgttcg gaaagaaaag cgcgcacttc cgcttccgtt gcgccctcaa
     3481 cccatgcgct acgatatggc ggcacagcac attcatcgcc gataaacaac gcattgtaat
     3541 cggcagagac ttgcgtcata tcacagcttt tctgtaaacg cgtcagtaac tcatcctgct
     3601 ccagtggcca gttcgcagcc agtttcccct cacgaatcag ggtaaacaac ggcaccagta
     3661 aaggatcttg cggttggcgg taatagagcg aacccagcac acgacagagg atagaaaact
     3721 cattcattaa tatatttcca ttactagtaa ttaaaggtct gcaaattccg caatcggtgc
     3781 cataccgcga gattcaagga agttaagtaa gcggcgcgga gaaacattca aaatgcgctc
     3841 tggcggaaaa tctaccgcgt cgaggatttt aagacactct tcaaattccc ccatggtaaa
     3901 cgcagtgtga gaatccgagc ctaatgccac ccaacctcca gcgtcacgta ccgctgcggc
     3961 taccgcccga cagttgtctt cactgccctt acgtgagtgt aaaaatgagg aattattgat
     4021 ttccagcgcc acctgatgtt tcgcggctgc ttcagcaacg gctttaacat ctatttcata
     4081 tttcggattt ccgggatggc ttattatatg cacattgccg ctggcgatag tggcgatcat
     4141 cgcttgcgta ttggtcgctt tgtcatgcgg cgcaaacacc ggctcatgaa aaccggcaat
     4201 aatcagatcc agcgaatcaa acattttgcc actgcagtca atttcaccgt caacattttt
     4261 aatgttagct tcgatgccgc gcaggatccc aaccccatca accactcgcg gccagatacg
     4321 catgttaatg aagtgccagt gatgcggcgc atcttccata tccgggccat gatcggtgat
     4381 cgcaaaaagt ttaatgccct tttgtttggc ctgggcaatg taatcactca atgtgctata
     4441 tgcatgcgtg ctggcaacgg tatgcatatg aaggtcgacg ggatacataa ctctctcctg
     4501 tattcaattc ccgccaagga tagcaggaaa cctgatgctt tattagtagc cgcgtgcgcg
     4561 gtcgacttgc ccgcagaccc tctccccttt ttcgagctgg gcaatggtgc gcgaaatgta
     4621 atccacagct tcagcgggac gggtaatcgc ggcgacatgt ggtgttatcg tcacgcgtgg
     4681 atgttgccag agcggacttt ccggcggtaa gggttcacga ttaaaaacat ccagcattgc
     4741 gcctttaact ttgccgctat ccagcgccgc gagcaggtca tcttccacga catgaacacc
     4801 acgcgccagg ttgaggagat acgcgccatc cggtaatttt tcgagtaatt gttgattaat
     4861 aatgccgacg gtttcaggtg tattcggtaa caaattaatc aatacccgac attggctcag
     4921 aaatgcagag agttcttccc gtccggcaaa gctttgcacg ccaggccacg atttacgggt
     4981 tcgactccag caacgcagcg ggaagcgcca ggtttgcaga ctctgtgcaa ctttactgcc
     5041 cagtacacct gcacccaaaa tgccgatggt aaaatcttcc cgatgatatt caggcagcgg
     5101 ttgccaatgc gaactatttt gctggatgcg gtaatcgtca aaacgtcgaa accaatgcag
     5161 cacctgactg acagcatatt cctgcatttg ctcgcccata ccggtatctt ccaggcgaaa
     5221 aagtggaaca gaaggcttca gcatttcagg gtgagcctgt agcttgctca aaatagaatc
     5281 aacacctgcc ccaagtgcaa acaccgcttt aagatcgcgc ccagccagca tttcaacagg
     5341 aggatgccag actaaagcat aatcggctga ttcattgtct ccgcttttcc atgctctgac
     5401 tcttgcctga ggaatagctt tgtgcagtgc ctcaatccac cattgggtat cgaacgttgg
     5461 gtgataaaag atgatatcca tactgactcc cgaagagtgt tggcgaaatt tattcgcact
     5521 tatcgttatg atctacaaag accagcagca taacaaatcc gtggtcggtg gcaaaaaaag
     5581 caaatttcgc ttattaaaac tgcatattga ttgaagtttg aataatcgag cgatttttta
     5641 aaaaagtttg ttgacctcag gtcatgattt ccctaaatta gcgcccgttc cagcaagaca
     5701 ggaacgacaa tttggtgagg tgtccgagtg gctgaaggag cacgcctgga aagtgtgtat
     5761 acggcaacgt atcgggggtt cgaatccccc cctcaccgcc agattacaag cgcatgttat
     5821 tgtttaacat gcgctttttt atttatcctt ccagacagta tgccaccggg tatgccatct
     5881 gaaaccggtt tatatgcccc acgatcatat cttatcggac aatttccgat ctttgagcat
     5941 actggcaaag ctatactgcc agcatgtcag gatagtgttt tgcgataata tcattcagtc
     6001 tgtcaaccag ttctggcttc ctgaacgtga tgtgcgcaga gcccttctta aagtatttaa
     6061 taatgaacat ctcatcttca tagcattctt ttccctgaag agaccggatg tgatcgtcca
     6121 gatgaatagt tatgttttcc ctgttgtcgg ggatcggttt gccgctgaac agatgcagca
     6181 ttctttccag gtcggcaagt ctatctgcct gttgcccggt gataagatgc aatccccact
     6241 ggtcccacct caccagattg ttgacgataa ttttattgcc aaatttacag gggcaattgg
     6301 ttttgtaatt ccagctcaac cctctgaaga cgttgatcac tcctcgctca aacacttcat
     6361 ccttattctg gtgtagctgc tcaaacgtac tcagaatatt ctcttcactg atttccggaa
     6421 aattatcgta ctccagtgac cggtaccatg tatcacgggc ctgtgcatcc attaaggaaa
     6481 gcatcccgga gcattgcatc aggtcacgcc agatttctcg atcgagattg c
//
LOCUS       DAZGQL010000171         6342 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.172, whole genome shotgun sequence.
ACCESSION   DAZGQL010000171 DAZGQL010000000
VERSION     DAZGQL010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6342
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.172"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..370)
                     /locus_tag="ACHKIR_004838"
     CDS             complement(<1..370)
                     /locus_tag="ACHKIR_004838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001588797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO9992468.1"
                     /translation="MITYAGYKVKIIFIIFLFLGYHVVAYAEMGRDREFCYPGTPGNN
                     TTPATFFYDFGTVIISDVNNNSAGTVLPSQNWKIGRYKAYCNSLDDYEVYFSGISGID
                     PSGASGNPQGAEIFIPVTHEI"
     gene            complement(385..1164)
                     /locus_tag="ACHKIR_004839"
     CDS             complement(385..1164)
                     /locus_tag="ACHKIR_004839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO9992469.1"
                     /translation="MRQYRLLSPMIKVVIVLFSLLFSTAQAGLVIGGTRFAYPENQSS
                     ISVELKNTSDRDMLVKVAVSPDDARQLTGTVESLPSFSGTVFIATPPLFVLKPDKNTK
                     IRINRVGGSLPADRESLFILNVAALPSLENSHPTKTDNQLQIAVRNRMKIFYRPSNLS
                     EDPNISYQKLRWARKNEIVTVYNPGPRYVTLYNLHVDGKVMDGGMIAPFSHRQQSWCK
                     AQGICEITWQTLDIYNNVLPVWKVNMNPLQMDVSGSQFKTD"
     gene            complement(1130..1702)
                     /locus_tag="ACHKIR_004840"
     CDS             complement(1130..1702)
                     /locus_tag="ACHKIR_004840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO9992470.1"
                     /translation="MPGKQMLCCILISIISEGDMKIFISLFLFIISTNSFADDITHAG
                     VVRIEGLITEKTCIISDESKNFTVNMPDVPSSSVRSAGDVTEKVYFSITLTRCGSDVG
                     NAYIKFTGNTVSEDASLYKLEDGSVDGLALTIFDKNKGSISNDVKSIGFSLTSSVDNI
                     LHFFAAYKALKNNVQPGDANASVSFIVTYD"
     gene            complement(1653..2216)
                     /locus_tag="ACHKIR_004841"
     CDS             complement(1653..2216)
                     /locus_tag="ACHKIR_004841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO9992471.1"
                     /translation="MNLIFSKYSVFFILFAFFAFPLSGQANDSVVVTFTGILLAKSCD
                     ITTGSKDQSVKMGTYDANEFLNVGDVSPSQTFTINVQGCPTAKSTVYSSGVSANVRFT
                     GDADAINSALLRLSAGADSATGLGIEILDNNDVAIAINGESGFRDLVLNENGDANLTF
                     KLRYKSTQENVHAGQANALLYFDIDYQ"
     gene            complement(2216..4810)
                     /locus_tag="ACHKIR_004842"
     CDS             complement(2216..4810)
                     /locus_tag="ACHKIR_004842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HFO9992472.1"
                     /translation="MNLKLKRCEYWMAAQKQMKRVVPLLLVIMPACSIAGMRFNPAFL
                     SGDTEAVADLSRFEKGMTYLPGSYEVEVWVNDSPLLSRTVTFKADDENQLIPCLSLAD
                     LLSLGINKNALPEQALALSENSCLDLRIWFPDVHYMPELDAQRLKLTFPQAIIKRDAR
                     GYIPPEQWDNGITAFLLNYDFSGNNDRGDYSSNNYYLNLRAGINIGAWRFRDYSTWSR
                     GSNSAGKLEHISSTLQRVIIPFRSELTLGDTWSSSDVFDSVSIRGIKLESDENMLPDS
                     QSGFAPTVRGIAKSRAQVTIKQNGYVIYQTYMPPGPFEISDLNPTSSAGDLEVTIKES
                     DNSETVYTVPYAAVPILQREGHSKYSTTVGQYRSNSYNQKSPYVFQGELIWGLPWDIT
                     AYGGAQFSEDYRALALGLGLNLGVFGATSFDVTQANSSLVDGSKHQGQSYRFLYSKSL
                     VQTGTAFHIIGYRYSTQGFYTLSDTTYQQMSGTVVDPKTLDDKDYVYNWNDFYNLRYS
                     KRGKFQASVSQPFGNYGSMYLSASQQTYWNTDKKDSLYQVGYNTSIKGIYLNVAWNYS
                     KSPGTNADKIVSLNVSLPISNWLSSTNDGRSSSNAMTATYGYSQDNHGQVNQYTGVSG
                     SLLEQHNLSYNIQHGFANQDNSSSGSVGVNYRGAYGSLNSAYSYDNEGNQQINYGISG
                     ALVVHENGLTLSQPLGETNVLIKAPGANNVDVQRGTGISTDWRGYAVVPYATEYRRNN
                     ISLDPMSMNMHTELDITSTEVIPGKGALVRAEFAAHIGIRGLFTVRYRNKSVPFGATA
                     SAQIKNSSQITGIVGDNGQLYLSGLPLEGVINIQWGDGVQQKCQANYKLPETELNNPV
                     SYATLECR"
     gene            complement(4847..5599)
                     /locus_tag="ACHKIR_004843"
     CDS             complement(4847..5599)
                     /locus_tag="ACHKIR_004843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO9992473.1"
                     /translation="MKCIIGNKKWVYGFRDELKVSQNIIRSGILFLLSILFINNASAG
                     GFGLGVTRLIYPAESKQITLSVQNSGDNTSYLIQSWVDDSKTEKKSQDFVITPPLFML
                     PTRKEASLRIMFLGKTNLPTDRETLYWMNVKAIPPTDEKNTQKNTLQLALQNKIKLFY
                     RPENLPVQPGKARNMLRFKYEGKQLKVINPSPYYLTLTGIKVQGSKLPNTFVPPKSDI
                     TVSSAVTLAGEITYQTINDFGATTERQKGIMQ"
     gene            complement(5700..>6342)
                     /locus_tag="ACHKIR_004844"
     CDS             complement(5700..>6342)
                     /locus_tag="ACHKIR_004844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO9992474.1"
                     /translation="AVTAAPGDAAQFGGADTDWSTVDYPRLTDMDDNVDSMGGKIRFT
                     GRVVKATCKVATDSKQIEVVLPVVPSNLFTGIDIEAQGASNQTDFNINLTECSNTEDQ
                     KIEFRFTGTADSANKTLANEVEGSTDADNSGNAGATGVGIRIYSKGTTNNGLINLNTT
                     AAEGSASTAAYTIPGNATTHDFSAAFTAGYAQNGSTVAPGVVKSTASFVVLYE"
BASE COUNT         1893 a         1410 c         1164 g         1875 t
ORIGIN      
        1 atatttcatg cgtaacagga ataaatattt ctgctccctg tggattacct gaagcacctg
       61 aaggatctat tcctgaaata cctgaaaaat aaacttcata atcatcaaga gaattacagt
      121 atgctttata tcgacctatt ttccagtttt gagatggtaa aacagtaccg gctgaattgt
      181 tgttaacatc tgaaataata actgtgccaa aatcataaaa aaaggtcgct ggcgtagtat
      241 tgtttcctgg agtaccagga tagcaaaact cacgatcccg gcccatttca gcataggcta
      301 cgacatggta ccctaagaat aaaaaaatta taaaaataat tttcacttta tatccggcat
      361 aagtaatcat acgatttcct tttactaatc cgttttaaat tgtgagcctg acacatccat
      421 ttgcaacgga ttcatattta ctttccatac aggcaataca ttattataaa tgtccaatgt
      481 ctgccatgtt atttcacaaa tcccttgcgc tttacaccat gattgttgac gatggctaaa
      541 tggtgcgatc ataccaccat ccataacttt accatcaaca tgaaggttat agagtgtcac
      601 atatctgggg cctggattat aaacggtaac aatttcattt tttcgtgccc agcgtaattt
      661 ttgatatgaa atattcgggt cttcagacaa atttgatggc cgatagaata ttttcatacg
      721 attgcggacc gctatttgca attgattatc agttttggtt ggatgactat tttccaacga
      781 tggtaatgca gcaacattaa gtataaataa actttcccgg tcggcaggta aactgccacc
      841 aacacgatta attcttattt ttgtattttt gtcaggcttc aaaacaaaca gtggcggagt
      901 tgcgataaat accgttccag aaaatgaggg caaagattca accgttcccg tcaactgacg
      961 agcatcatca ggggagactg caaccttgac taacatatct ctgtctgaag tgtttttgag
     1021 ttcaacagat attgaggact gattttccgg atacgcaaaa cgcgttccac cgatcactaa
     1081 tcctgcttgt gctgtagaga ataaaaggga aaataatact atgactacct taatcatagg
     1141 tgacaataaa cgatactgac gcattggcat cccctggttg gacattattt tttaatgctt
     1201 tgtaagccgc aaaaaaatgc aatatattat caactgatga tgtaagtgaa aaacctatgc
     1261 ttttaacatc attactaata ctgcctttgt tcttatcaaa aatcgtgagt gcaagcccat
     1321 ctaccgagcc atcttccagc ttatataaac tggcatcttc agaaactgta ttgccggtaa
     1381 actttatata cgcgttgcca acatcactac cacagcgggt taacgttatg gaaaaataaa
     1441 ccttttcagt aacatcccct gcactcctta ccgaactact gggtacgtct ggcatattaa
     1501 ctgtaaaatt ttttgactca tcagaaataa tgcaggtttt ttcggtaatt agcccttcaa
     1561 tacgaaccac tccggcatga gtgatatcat cagcaaaaga atttgttgat attataaaca
     1621 aaaataaact aatgaatatc ttcatatctc cctcactgat aatcgatatc aaaatacaac
     1681 agagcatttg cttgcccggc atgtacattt tcctgagttg atttatagcg aagtttaaac
     1741 gtcaaattag catcaccgtt ttcatttaac actaaatcac gaaaacctga ttcaccatta
     1801 atagcaatcg ccacatcatt gttatccaat atttctattc ccagtccagt cgcactatct
     1861 gcaccggcgg agagacgcag taatgcgcta ttaatggcat ctgcatcacc agtaaaccgc
     1921 acattggcgc tcacaccaga actataaaca gtactttttg cagtcgggca tccttgtaca
     1981 ttaatcgtaa acgtttgact cggagaaaca tcacctacat ttaaaaattc attagcatca
     2041 taagtgccca tttttaccga ctgatcttta cttccggtag taatgtcaca gctttttgcc
     2101 aacaaaatcc cagtaaatgt aacaacaact gaatcattgg cctgccctga gagcggaaaa
     2161 gcaaaaaaag cgaaaagaat aaaaaataca gaatatttag agaatattaa attcattagc
     2221 ggcactccag agtagcatag ctaacaggat tattcagttc tgtttcaggg agcttgtaat
     2281 tagcctgaca tttttgctga acaccatctc cccactggat attaataaca ccttctaaag
     2341 gcaatcctga gagataaagt tgtccattat cgccgacaat cccggtaatt tgactactat
     2401 ttttaatctg tgcgctggct gtagcaccga atgggactga tttattacga taacgaactg
     2461 tgaacaaacc acgaataccg atatgagcag caaactctgc acgaactaac gcaccttttc
     2521 ccggaataac ttcagtggaa gtgatatcca gttcagtatg catattcatt gacataggat
     2581 ctaatgaaat attattacgt ctatattctg ttgcataagg aacaactgca tatccacgcc
     2641 agtcagtgga tattcctgtc ccccgctgga catccacatt attcgctcca ggcgctttta
     2701 tcaaaacatt agtttcacct aatggttgac tcaacgtaag accattttca tgtacaacaa
     2761 gagcaccact gatgccatag tttatctgtt gattaccttc attatcgtaa ctgtaggcgg
     2821 aattcaagga accatatgcc ccacgataat taacaccaac agaaccacta ctgctattat
     2881 cctgattagc aaaaccatgt tgtatgttat aactgagatt atgctgctcc aacagagaac
     2941 cagatacccc cgtatattgg tttacctgtc cgtggttatc ctgactataa ccatacgttg
     3001 cagtcatggc attcgatgat gagcgcccat cattcgtgga agataaccaa ttacttatag
     3061 gtaatgagac atttagcgag acaattttat ccgcatttgt ccctggtgat ttactgtaat
     3121 tccacgcaac atttagatag atacccttaa tactggtgtt ataaccaact tggtataaag
     3181 aatctttttt atcagtattc cagtatgttt gctgactagc cgataaatac atagacccgt
     3241 agttaccgaa aggttgcgat acactagcct gaaattttcc acgtttgcta taacgcaagt
     3301 tataaaaatc attccagtta taaacgtaat ctttatcatc taacgttttt ggatcaacaa
     3361 cagtccctga catttgttgg tatgtcgtat cacttaaagt gtaaaagccc tgggttgaat
     3421 aacgatagcc aataatatgg aatgctgttc ctgtctgaac taacgatttg gaataaagaa
     3481 aacgataaga ttgcccttga tgtttgctcc catccacaag cgaactgtta gcctgagtaa
     3541 catcaaacga tgttgcacca aatacaccca gattcaggcc aaggccgagc gccaacgccc
     3601 ggtaatcctc agagaattgt gccccaccat aagccgtaat atcccagggt aaaccccaaa
     3661 ttaattcccc ctgaaataca taaggacttt tctggttata gctattgctt cgatattggc
     3721 caaccgtagt agaatatttt gaatgacctt ctcgttgcag gatggggaca gcggcataag
     3781 gtacggtata gacagtttct gaattatcag actctttgat ggtaacttcc agatctcccg
     3841 cagatgatgt cgggttaaga tcgctaatct caaacggtcc cggcggcata taggtttgat
     3901 aaatgacata accattctgt ttgattgtta cctgagcgcg acttttcgca attccgcgca
     3961 ccgtgggagc gaaaccactt tgactatcgg gcaacatatt ttcgtcagat tccagtttta
     4021 tgccacgaat actaacactg tcgaaaacat ctgatgatga ccatgtatct cctagcgtta
     4081 attcacttct gaaaggaata ataacgcgct gcaacgtact actgatatgc tccagtttac
     4141 ctgctgaatt actcccacga ctccaggttg aataatcgcg aaaacgccat gcaccaatat
     4201 tgatcccagc gcgaagattt aaataatagt tatttgaaga gtaatcacca cgatcgttat
     4261 taccagaaaa gtcataattc agcaaaaaag ctgtaatacc gttatcccac tgttctggtg
     4321 gaatatatcc gcgggcgtca cgttttatta tcgcctgcgg aaaggtcagt ttaagtctct
     4381 gtgcatccag ctccggcatg taatgcacat caggaaacca gatacgcaaa tcaaggcaac
     4441 tattttcaga taaagccaaa gcctgctctg gcagcgcatt cttgttaatt ccaaggctta
     4501 ataagtcagc aagtgaaagg cagggaatca gttgattctc atcgtctgct ttaaaagtta
     4561 cagtacgaga gagtaaaggg gaatcattga cccaaacttc gacttcatag ctaccaggaa
     4621 gataagtcat ccctttctcg aaacgggata agtcagcaac agcttcagta tcacccgaca
     4681 gaaaagcagg gttaaagcgc attcccgcga ttgaacatgc aggcataata accagaagaa
     4741 gcggtacaac ccgtttcatc tgcttttgtg ccgccatcca atattcgcat cttttgagct
     4801 ttaggttcat atccctgacc tgtgttgcat ctgaactatc ccgttgttac tgcattattc
     4861 ctttctgccg ctccgtcgta gcgccaaagt cattaattgt ttgataagta atttcaccag
     4921 ccaacgtcac tgccgacgag accgtaatat cgcttttcgg tggtacaaac gtattgggca
     4981 atttactccc ttgtacttta attcctgtca gtgtcaggta ataaggcgaa ggattgataa
     5041 cttttagttg tttgccctca tacttaaacc gcaacatatt tcgtgctttt cctggttgca
     5101 caggtagatt ttccgggcga taaaatagct tgattttgtt ttgtaatgcc aactgtaagg
     5161 tattcttctg tgtatttttc tcatctgttg gcggaattgc tttaacattc atccaataca
     5221 aagtttcacg atcggtcggc aaattagttt taccaagaaa catgatacgt aatgaagctt
     5281 ctttacgcgt tggcaacata aagaggggag gagtgataac aaaatcctgg ctcttttttt
     5341 ccgttttact gtcatcaacc catgattgaa ttaaataact ggtgttgtca ccgctatttt
     5401 gcacactgag tgttatttgc ttactttctg ccggataaat caatcgcgtt acgccaaggc
     5461 caaacccgcc cgcggacgca ttattaataa ataatatcga tagtaaaaaa agaatccctg
     5521 agcgtataat attttgtgat acttttaatt catccctgaa tccataaacc cattttttat
     5581 ttccaataat acatttcata cagcatcctt gttgatgaaa taacacctgt tattcatact
     5641 ctattaaata atttcaatct caggttacaa ctttttgtaa cctgaaatcg tatttatatt
     5701 tactcgtaca gcaccacaaa acttgctgtt gacttaacta caccaggtgc aacagtgcta
     5761 ccgttttgag cataacctgc agtaaaggcc gcgctgaaat catgggtcgt agcatttcct
     5821 ggaattgtat aagcggcggt ggaggcgcta ccctctgccg cagtagtatt caggttaatc
     5881 agaccattat tcgtcgtacc tttggagtaa attcgaatcc ctacaccagt cgcccccgca
     5941 ttgccgctgt tgtcagcatc cgttgatcct tctacttcgt tagcgagcgt tttattagcg
     6001 ctatctgcgg taccggtaaa gcggaactca attttctgat cctctgtatt gctacattcg
     6061 gtcagattaa tattgaaatc ggtctggttg ctcgccccct gtgcttctat gtcgatacca
     6121 gtgaaaaggt tggaaggcac aaccggcagg acaacttcaa tctgttttga atcggttgcg
     6181 accttacagg tagctttcac tacacggcca gtaaagcgga ttttcccccc cattgaatca
     6241 acgttgtcat ccatatcagt gagcctggga taatcaacgg tgctccagtc agtatccgct
     6301 ccaccaaatt gtgctgcatc gccgggtgct gcggttaccg ca
//
LOCUS       DAZGQL010000172         6229 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.173, whole genome shotgun sequence.
ACCESSION   DAZGQL010000172 DAZGQL010000000
VERSION     DAZGQL010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6229
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.173"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            241..456
                     /locus_tag="ACHKIR_004845"
     CDS             241..456
                     /locus_tag="ACHKIR_004845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001498920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO9992475.1"
                     /translation="MSQQKITGIDLAKTNFYLFSINAHGKPAGRIKLSRNQLLNWLVQ
                     QPKMTVAMEARGHPITGPGKSGNSTMM"
     gene            630..887
                     /locus_tag="ACHKIR_004846"
     CDS             630..887
                     /locus_tag="ACHKIR_004846"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992476.1"
                     /translation="MERTQLINHVRGLLAEYGIVFSKGATELRQKLPALLEDAENELT
                     DTMKTLLHRQYIRLITLDNELEWYDSELKNMSAKILCANAC"
     gene            869..1060
                     /locus_tag="ACHKIR_004847"
     CDS             869..1060
                     /locus_tag="ACHKIR_004847"
                     /inference="COORDINATES: protein motif:HMM:NF014430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO9992477.1"
                     /translation="MCKRLLAIPRFSPLVSQAIKSWMGDGKQFRRGRDASATLGLVPR
                     QFSTGGKQVLLGITKFGEM"
     gene            1202..2221
                     /locus_tag="ACHKIR_004848"
     CDS             1202..2221
                     /locus_tag="ACHKIR_004848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFO9992478.1"
                     /translation="MKYTPVGVDIAKHLIQVHFIDEHTGEVIDKQLRRQDFLMFFCNR
                     EPCLIGMEACRGSQHWGRELTKLGHKVRLLQAHFVKAFVMGNKNDVMDARAIWMAVQQ
                     PGKDIAVKTEEQQSVLVLHRSRRQLVKFRTMQINSLHGTLLEFGKTIHKGRAAMEREL
                     PEALERMKERLPPYLITVLENQYNRLNELDSLIEDIEKKLTSVARQNETCKRLLDIPG
                     VGPLIATAAVATMREASAFKSGREFAAYVGLVPKQTGSGGKIRLLEISKRGNTYLRAL
                     FIHSARAAALLPKEPGPWITELKKRRPTSVAIVAMANKLARTVWAIAAHGSKYDKNHV
                     SIRSY"
     gene            2942..3157
                     /locus_tag="ACHKIR_004849"
                     /pseudo
     CDS             2942..3157
                     /locus_tag="ACHKIR_004849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612066.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            3332..5302
                     /locus_tag="ACHKIR_004850"
     CDS             3332..5302
                     /locus_tag="ACHKIR_004850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent
                     hemoglobin/transferrin/lactoferrin family receptor"
                     /protein_id="HFO9992479.1"
                     /translation="MYMNVIRTVICTLIILPVGLQAATSHSSMAKDTITVVATGNQNT
                     VFETPSMVSVITNDTPWSQNAVTSAGMLKGVAGLSQTGAGRTNGQTFNLRGYDKSGVL
                     VLVDGVRQLSDMAKSSGTYLDPALIKRIEVVRGPNSSLYGSGGLGGVVDFRTADAADF
                     LPPGETNGVSLWGNIASGDHSTGSGLTWFGKTGKTDALLSVIMRKRGNIYQSDGERAP
                     NKEKPAALFAKGSVSITDSNKAGASLRLYQNNTTEPGNPTLTHGDNGLRDRKTAQNDV
                     QFWYQYSPADNSLINVKSTLYLSDITIKTNGHNKTAEWRNNRTSGVNVVNRSHTLIFP
                     GAHQLSYGAEYYRQQQKPEGSATLYPEGNIDFTSLYFQDEMTMKSNPVNIIVGSRYDR
                     YKSFNPRAGKLKAERLSPRAAISVSPTDWLMMYGSISSAFRAPTMAEMYRDDVHFYRK
                     GKPNYWVPNLNLKPENNITREIGAGIQLDGLLTDNDRLQLKGGYFGTDARNYIATRVD
                     MKRMRSYSYNVSRARIWGWDMQGNYQSDYFDWMLSYNRTESMDASSREWLGSGNPDTL
                     ISDISIPVGHGGVSAGWRAELSAAATHVKKGDPHQAGYAVHSFSLSYKPVSVKSFEAS
                     VTLDNAFNKLAMNGKGVPLSGRTVSLYTRYQW"
     gene            5321..6112
                     /locus_tag="ACHKIR_004851"
     CDS             5321..6112
                     /locus_tag="ACHKIR_004851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HFO9992480.1"
                     /translation="MMKNTGYILALCLTASEHALAHDVWITGKQAENNVTAEIGYGHN
                     FPSKGTIPDRRNFFENPRLYNGKEIITLKPTSTDYVYKTESAANGNSYVLSTKMKPGY
                     WSRTSSGWKPVSREGRNDVAYCEFVTKYAKSFIPGEQQMPAQLYQSPTGDELEIIPLS
                     DISRFGEDVKLKVLYKTSPLAGATLELDSVSYLKSSRHTHAAEHKHSAHKAELTFVSN
                     EDGIITVPSLHVGQWLAKVKNKKVFPDKSLCDETVDVATLSFSRN"
BASE COUNT         1850 a         1378 c         1504 g         1497 t
ORIGIN      
        1 acactcacaa gggaacatag attcaatgcg tttatttaga aggtctgtct tctttttaat
       61 attaacttat taattggtgc tattcatata attctttaaa cagcgaactt atagccagga
      121 ttcaatagat atagtaacag gaacatcagt aagcgatgag atggtcttcc ccctccttca
      181 cgacaacctt taattacgtc atgatggata aactttaacc acaaaagtaa gagggatacc
      241 atgtctcaac agaaaatcac cggcatcgat ctggctaaaa cgaactttta tcttttcagt
      301 atcaatgctc atggcaaacc cgcaggaagg atcaaacttt ctcgcaatca actgcttaac
      361 tggctggtgc agcagcctaa aatgactgtt gcgatggagg ctcgcgggca tcccatcact
      421 gggccaggga aatcaggaaa ctcgaccatg atgtaatatt tcttccggcg caacatgtta
      481 aagcttatca gcgacgccag aaaaatgatt atgacgacgc tcaggcaata gctgaggcct
      541 gccaacatgg aactatacgc cccgtaccaa taaaaactct ggagtaacag gacgtccaga
      601 cttttttgaa aatgcgccgg ttggtttcga tggaaaggac acaactgata aatcacgttc
      661 gtggacttct tgctgaatat ggcattgtgt tcagtaaagg cgcaacagaa ttacgccaaa
      721 aattaccggc attacttgag gatgcggaaa acgaactcac tgataccatg aagacgttat
      781 tgcatcggca gtatatacgg ttgattacac tagataatga actggagtgg tatgattctg
      841 aactgaaaaa tatgtccgcc aagatcctat gtgcaaacgc ttgttagcca ttcctagatt
      901 cagccctctg gttagtcagg caataaaaag ctggatgggg gatggaaaac agtttagacg
      961 aggaagagat gcatctgcga ctctgggact ggttccccga cagttcagca caggtgggaa
     1021 gcaggttctt ctgggtataa caaaatttgg agaaatgtaa tgaacacatc ccaccccatt
     1081 aagtcaggtg ctctgtgatg gatgaaggga aatactggaa ggtgtggagc ctcgcagatt
     1141 tccggcatca tagtgcccaa tacaggatgc ataaagctcc ctcagactgg agactccaca
     1201 aatgaaatat acaccggttg gcgttgatat cgcaaaacat ctgattcagg ttcatttcat
     1261 cgacgagcac acaggtgaag tgattgataa gcaactacgt cgtcaggatt ttctgatgtt
     1321 cttctgtaat cgggaaccct gcctgattgg tatggaggcc tgtagaggct ctcagcactg
     1381 ggggcgtgaa ctgacaaagc tggggcataa agtccggttg ttacaggccc actttgttaa
     1441 ggcattcgtc atgggaaata agaatgatgt gatggatgcc cgggctatct ggatggctgt
     1501 tcagcagcca ggtaaggaca tcgcggttaa aacagaagaa caacagtcag tgctggtttt
     1561 gcaccgttcc cgcagacaat tggttaagtt ccgcaccatg cagattaatt ctctgcacgg
     1621 cacgctccta gaatttggta aaaccattca caaaggtcgg gcagcgatgg agcgagagct
     1681 ccctgaagca ctggagcgga tgaaagagag actgccgccg tatctcatta ctgtgctgga
     1741 aaaccagtac aaccggctga atgagctgga ctcgctgata gaggatattg aaaaaaagct
     1801 taccagcgtg gcgagacaga atgaaacctg taagcggttg ctggatattc ccggcgttgg
     1861 gccacttatt gcaacggcag cggtggccac catgagggaa gcatcagcgt ttaaatcagg
     1921 gcgagagttc gcggcttatg ttggtctggt gcccaaacag actggctcag gagggaaaat
     1981 ccgcctgctg gagataagca aacgtggtaa tacatatctg cgagccttgt ttattcatag
     2041 cgcaagggcg gcagcattac tgcccaaaga acctggtccg tggataaccg aactgaaaaa
     2101 acgtcgtcca accagcgtgg caatcgtcgc catggcaaac aaactagcac gaacagtatg
     2161 ggcgatagca gctcatggaa gtaaatacga caagaatcat gtcagtatca gatcatatta
     2221 atcgctgata ccgataaata ttaactttta atgaaaggat gaatactgaa aggtaactct
     2281 ggcggccata gtgatgacaa cgacaggtaa gaccgggact cactaaacct gaatattgtg
     2341 ttgagctaaa agctcgctgg agaaatgagg tgtgagtcag cgaattacat aggggctcgc
     2401 agcttcatgg ctgcaaaaaa gccggatata aagctgcaac ctacccgtca ctgtcaaggc
     2461 aatggatgcc ttgcaacggg atgtgttcat ataatcaata tgtcagatct atgctgatac
     2521 agggtgccag agcggtattg ttcagagcct cagagaaaac tgattcactt cgtgtgtggg
     2581 ttaattgtat tcgggaaaag cgtggcttca accaggcagt tgttgctctt gcaaataaac
     2641 tccttcggat cgcctgggca attatcgccc gcaatgagat gtatttgccc gcagcaaaac
     2701 aaaatcaata atctacgtaa aatcagtagg agtaaagctt accctgaaat tgcaaagaat
     2761 gcagaatgat gaaaaacagg tcagaccgac gttacgaaaa cctgcaagat gagccaggcc
     2821 agtaaggccg agtaagcgat aaggtcggag cgcgcgaatg tctcatcaag gccagaagca
     2881 ataacttcat gaacaaaccg gatatacgaa agtaaacctg tccctatcat agttgctgta
     2941 ctcttgcaat acgggggaag accatctcat cgcttacgaa caattcgtga aaacgatgaa
     3001 gaaagactac atcgcgtgca tgccgaaatc taatgtaaga acggcattgc ataatcttgc
     3061 agtggagatc gggtattaca atgaaaacca tccgcatagt gcgttaggtt atctcccacc
     3121 aagggaatat caacgtcagc gaataacgtc accttagatg cagtagttgt caggaaatag
     3181 tgactcaagg gcaggcttac catatacact cttagtggtt cgactgggta ttagaatagc
     3241 tattaacaga ttagcactga acactgcgta atagtggttg cacaaacaaa atgttatgtt
     3301 ataacattgc tgtattgaca tatattttcg aatgtatatg aacgtaatca gaactgtcat
     3361 ttgtacatta atcatacttc cggtggggtt acaggcagcg acaagtcatt cttctatggc
     3421 taaagataca atcaccgttg tcgcgacagg aaatcagaac acggtatttg aaacgccgtc
     3481 gatggtcagt gtcatcacga atgacacgcc atggagtcag aatgcggtca catcagccgg
     3541 tatgctgaaa ggtgttgcgg gtctcagcca gactggtgca ggacggacca atgggcagac
     3601 ctttaatcta cgcggctatg acaaaagcgg ggtacttgtt cttgttgacg gcgtccgtca
     3661 actcagtgac atggcaaaaa gcagtggcac ttatctggat ccggcactca tcaaacgcat
     3721 cgaagttgtc cgcggaccaa actccagtct gtacggcagt ggcgggctgg ggggggtggt
     3781 ggacttcaga actgccgatg ctgcagattt tcttcccccc ggagagacaa atggtgtaag
     3841 tctgtgggga aatatcgcca gtggtgacca cagcacaggc tcggggctca cctggttcgg
     3901 taaaaccgga aaaacagatg cgcttctttc tgtcattatg cgtaaaagag gaaatatcta
     3961 tcagagtgat ggtgagcgtg cacctaacaa ggaaaaacct gcagccctgt ttgcgaaagg
     4021 ctctgtcagt ataacagaca gtaacaaagc aggtgccagc ttgcgtctct accagaataa
     4081 caccactgaa ccgggcaatc ccactctgac acatggtgac aacggcctgc gtgacagaaa
     4141 aacagcacaa aatgacgtgc agttctggta ccagtactcc cctgcggata acagcctcat
     4201 caatgtaaag tcaacgttat atctcagtga tatcactatc aagacaaacg gtcacaacaa
     4261 aacagcagaa tggcgaaaca acagaacctc cggtgttaat gttgtcaaca ggagccatac
     4321 tctgattttt ccgggagccc atcagttaag ttatggcgcc gaatattacc gtcagcagca
     4381 gaagccggaa ggctctgcca cactatatcc ggaaggaaac attgacttta catcgttgta
     4441 tttccaggat gaaatgacaa tgaaaagcaa cccggttaac attatcgtcg gttcccgtta
     4501 tgaccggtac aagagcttca atccccgagc cggaaaactg aaagccgaac gcctgtcccc
     4561 cagggcggcg atttcagtct caccaacaga ctggctgatg atgtacggct ccatatcctc
     4621 tgcattccgt gcccccacaa tggcagaaat gtacagggat gatgtacatt tttaccgcaa
     4681 gggtaaaccc aattactggg tacctaacct taatctgaaa ccagaaaata acattacccg
     4741 tgagattggc gcaggtattc aactggacgg cctgcttaca gacaatgacc ggctgcagtt
     4801 gaaaggcgga tatttcggaa cggatgccag aaactatatt gccacgcgcg tggatatgaa
     4861 acggatgcgt tcttattctt ataatgtatc ccgggcccgt atctggggat gggatatgca
     4921 gggtaattac cagtctgatt attttgactg gatgctttct tataaccgca cggaaagtat
     4981 ggatgccagt agcagggaat ggctgggctc cggcaatcct gacacactca tcagtgacat
     5041 cagtatacct gttggtcatg gaggagtttc tgccggatgg cgtgctgaac tttcggcagc
     5101 agccacgcat gtgaaaaaag gcgatcccca tcaggctggc tatgccgtac attccttttc
     5161 actgtcttat aagcctgtaa gtgttaaaag ctttgaggca tcagtgactc tggataatgc
     5221 cttcaacaag cttgccatga atggcaaagg tgtgccgctt tcaggcagaa ctgtcagtct
     5281 ttatacccgt tatcagtggt aacctcgcag gaaataaatc atgatgaaaa atacaggcta
     5341 tatcttagct ctttgtctga cagcatcgga gcatgccctg gcccacgatg tctggattac
     5401 cggtaaacag gcagagaaca acgttaccgc agagattggt tatggtcata atttcccctc
     5461 aaagggcaca attcctgaca gaaggaattt ctttgaaaat ccccggcttt ataacgggaa
     5521 agagataata acgctgaagc caacgtccac ggattatgtc tataaaactg agtctgcagc
     5581 caacggtaat agctacgttc tgtcaacgaa aatgaaaccg ggatactggt cgagaacctc
     5641 gtcaggatgg aagcctgtca gtcgggaggg cagaaatgat gtggcatact gtgagtttgt
     5701 gactaaatat gcaaaatctt ttattcctgg tgaacagcag atgccagcac aactctatca
     5761 gtctccaaca ggggatgagc ttgaaatcat tccattatcc gatatcagtc gtttcggtga
     5821 agatgtgaag ctgaaagttc tgtataaaac atccccgctt gccggagcta ctctggagct
     5881 tgactctgtc agttatctga aatcatcccg tcatacccat gcagctgagc ataaacattc
     5941 ggctcataaa gcagaactta cctttgtaag taatgaggac ggtatcatca ctgtaccttc
     6001 ccttcatgtc gggcaatggc tggctaaagt caaaaataag aaagtatttc cggacaagag
     6061 tctgtgtgat gaaactgtcg atgtggcaac cttaagcttc tcccgaaatt aataatacta
     6121 ttcctgccct gatttatggg cagggtatta tcaatacgag aatagcctac cgacatccgt
     6181 gtaaaaaacc acataatgat ttttcatatc catttccaga tatacttat
//
LOCUS       DAZGQL010000173         6215 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.174, whole genome shotgun sequence.
ACCESSION   DAZGQL010000173 DAZGQL010000000
VERSION     DAZGQL010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6215
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.174"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            122..1673
                     /locus_tag="ACHKIR_004852"
                     /pseudo
     CDS             122..1673
                     /locus_tag="ACHKIR_004852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019324.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MchC protein"
     gene            1699..2151
                     /locus_tag="ACHKIR_004853"
     CDS             1699..2151
                     /locus_tag="ACHKIR_004853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-activating lysine-acyltransferase"
                     /protein_id="HFO9992481.1"
                     /translation="MSYIRETIRGKDEWTVYEQIGFAVSCMLYNRNYSLYPVLTIQYW
                     TEYAIQHNQIKFLFDSRGFPLAYITWAYLEADTEARLLRDPEFRLHPSEWNEDGRIWI
                     LDFCCKPGFGRKVIDYLIQLQPWGEGEVRWLSRRKKIVTFIPERLHKT"
     gene            2304..3578
                     /locus_tag="ACHKIR_004854"
     CDS             2304..3578
                     /locus_tag="ACHKIR_004854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFO9992482.1"
                     /translation="MFRQDALENRKMKWQGRAILLPGIPLWLIMLGSIVFITAFLMFI
                     IVGTYSRRVNVSGEVTTWPRAVNIYSGVQGFVVRQFVHEGQLIKKGDPVYLIDISKST
                     RSGIVTDNHRRDIENQLVRVDNIISRLEESKKITLDTLEKQRLQYTDAFRRSSDIIQR
                     AEEGIKIMKNNMENYRNYQAKGLINKDQLTNQVALYYQQQNNLLSLSGQNEQNALQIT
                     TLESQIQTQAADFDNRIYQMELQRYELQKELVNTDVEGEIIIRALTDGKVDSLSVTVG
                     QMVNTGDSLLQVIPENIENYYLILWVPNDAVPYISAGDKVNIRYEAFPAEKFGQFSAT
                     VKTISRTPASTQEMLTYKGAPQNTPGDSVPWYKVIAMPEKQIIRYDEKYLPLENGMKA
                     ESTLFLEKRRIYQWMLSPFYDMKHSATGPLND"
     gene            3571..5672
                     /gene="mchF"
                     /locus_tag="ACHKIR_004855"
                     /pseudo
     CDS             3571..5672
                     /gene="mchF"
                     /locus_tag="ACHKIR_004855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181296.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="microcin export peptidase/ABC transporter MchF"
     gene            complement(5733..6056)
                     /locus_tag="ACHKIR_004856"
     CDS             complement(5733..6056)
                     /locus_tag="ACHKIR_004856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001390454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992483.1"
                     /translation="MRNITTDELALIHGAGLDKDASVAIGTAAGGFLGKATKIPSADI
                     AGSAIGGYIGGVIADGSNRTITIPSNGINYNPSIGLGSFNPNYNGGLLNSGFNSSTSS
                     SSSGS"
BASE COUNT         1811 a         1165 c         1471 g         1768 t
ORIGIN      
        1 aattttttaa tttatgtgta attaatagag ttttactgta ataaggaaga agctgtcagg
       61 acacttactt gctcttttat gaacactctt ttatgaaaat tgaaacggag aatactgcca
      121 gatgagtcat cagtgttcac tttctgaact gaatgaaaac ctggtgcctt tcactgccag
      181 gcagatcaag gcctcattaa tctggtgtgc agaggatgtc agaaatccag acgagctgca
      241 aaatgcctgc agttatatta tcgatcctgg cagtacagct tctgccaaag tgttccatgc
      301 agagcgctat ggtggcagtg gtattcagcg taatggaggt ggtgcacgtt gtgggtttga
      361 tggtaactac caggttaaag gaataggaag taatccgttg gttggcgaag gtactgacga
      421 acgtcattct aatggtgcac tcggcgctgt tcatgcaata ttatgaggct ttgtggggag
      481 aagtactggc tcaaatatta ccttatagtg ctgtgcgggt tcgggcggtt ttacttacag
      541 atctctatac tgaaaaggca tttgagcgct ccggtaggaa atcacgaaga gccctgttgg
      601 tacgtgagcc tgttgttcgt ccggcgcatt ttgaacgggc accttacttc caagtaaaac
      661 cggagtattc cagtctgcta attcacgatg cctgtcgggt tagatctgtg atccacaagc
      721 tgccaggata tctacctgta ccaccggaag aaattgatgc tgaagcacga actgatcccc
      781 ggatttattg cattgagggg ttatgtgaac tggcacgtcg tgaggcctgg caaatggcat
      841 tttgtcgaac acgtttcctg agattgacaa cttctccttc taatattgca atggatggca
      901 gattaatgga ttttaacgga ctcagttgtc tgtttccggg agattcccca gctgattttg
      961 ggtataaact aagattagct gaactggcaa aagaaccgat ggtacttatg caagggctgt
     1021 ctgatctctg cttgtatatc ggaaaatata tgtttgaccc tgacttcact cttgcagccc
     1081 gtttgaaggt tgaggagata tttcagaaaa ctttccatga agcatgttat tactgttatc
     1141 tagaactgtt gggtattcct ggagaattta taacacaaaa agagatacct gatatattga
     1201 aagaactggt taacagtttt gttgcattac tcaatcaata ctgcgagaaa tcacatgccc
     1261 aagatattgt caatcaggat ggttcaccat tgcaaaagtt ggttgtgacg ctaatccatc
     1321 ataggcataa tcaaaagcag gcactgaata gtagcatcaa gaatgatgtt tatttcaccg
     1381 ttgcacaaca gtgtttttcc caagccatcc actggctgac gcaaggcagt accagacgtc
     1441 agataaatgc ctcattactc ctgaaagaaa ttgaacatca taccatgaaa aggctgcaac
     1501 ccagggaaga gctgaggaaa gaaaatatgt gcgaaaaaat tgccatcctg ctggataatc
     1561 atggcgatga tccccttttt ttacaagaag caatttctga tatgaaaaat tttatgctta
     1621 agttttccag agatgcattt ggatatcttg aaccgataag aaacacagtg taaaaaatat
     1681 ataagggaaa tagtaatcat gtcttatata agggaaacca tcagaggaaa agatgaatgg
     1741 actgtttatg aacagatcgg ttttgcggtc agttgtatgc tctacaatcg taattacagt
     1801 ctgtatccgg tgttaaccat tcaatactgg actgaatatg cgatacagca taatcagatt
     1861 aaattcctgt ttgattcacg aggttttcca ctggcgtata ttacctgggc atatcttgag
     1921 gctgatacag aagcgcgcct gctcagggat ccagaattca ggttgcatcc gtctgaatgg
     1981 aatgaagatg gaaggatttg gattctggat ttctgttgta aaccaggctt tggtcgaaaa
     2041 gttattgact atctcataca gcttcagcca tggggagaag gagaagtacg atggttaagc
     2101 aggcgaaaga aaattgtgac attcatccct gagcggctgc ataaaacgta gtacctctga
     2161 agatacagag ataattgtaa attacggggt aaatgcatcg ctgatactat tttgacagga
     2221 ctctgtattt tctggctttg attatagcat ttcgcttaac cagtgaaggt attacaggaa
     2281 aagtgtgagt aagaggagct attttgtttc gtcaggatgc tttagaaaac agaaaaatga
     2341 agtggcaggg acgggcaata ttacttcccg gaataccact atggttaatc atgctgggaa
     2401 gcatagtgtt tattacggca tttctgatgt tcattattgt tggtacctat agccgccgtg
     2461 ttaatgtcag tggtgaggtc acaacctggc caagagctgt caatatatat tcaggtgtac
     2521 agggatttgt tgtcaggcag tttgttcatg aaggacagtt gataaaaaaa ggggaccctg
     2581 tttatctgat tgacatcagt aaaagtacac gtagtggtat tgtcactgat aatcatcgtc
     2641 gggatataga aaaccagctg gttcgtgtgg acaacattat ttcccgtctg gaagaaagta
     2701 aaaaaataac gctagatacc ctggaaaaac aacgcctgca atacacagat gcgtttcgtc
     2761 gctcatcaga tattatacag cgtgcagagg aagggataaa aataatgaaa aacaatatgg
     2821 agaattacag aaactatcag gcaaaagggc tgattaataa agatcagtta actaaccagg
     2881 tggcattata ttatcagcaa caaaacaatc ttctcagcct gagcggacag aacgaacaga
     2941 atgctctgca gataaccact ctggagagtc agattcagac tcaggctgca gattttgata
     3001 accgtatcta ccagatggaa ctgcaacggt acgagttaca gaaagaactg gttaacactg
     3061 atgtggaggg cgaaatcatt atccgggcgt tgactgacgg gaaagttgac tccctgagtg
     3121 tcactgtcgg gcaaatggtc aataccggag acagccttct gcaggttatt cctgagaaca
     3181 ttgaaaacta ttatcttatt ctctgggtcc cgaatgatgc tgttccttat atttcggctg
     3241 gtgacaaagt gaatattcgt tatgaagcct ttccggcaga aaaatttggg cagttctctg
     3301 ctacggttaa aactatatcc aggactcctg cgtcaacaca ggaaatgttg acctataagg
     3361 gagcaccaca gaatacgccg ggcgactctg ttccctggta taaagtcatt gcgatgcctg
     3421 aaaagcagat tatcagatat gacgaaaaat acctccctct ggaaaatgga atgaaagccg
     3481 aaagtacact atttctggaa aaaaggcgta tttaccagtg gatgctttct cctttctatg
     3541 acatgaaaca cagtgcaaca ggaccgctca atgactaacg ggaatttcag acaaattata
     3601 aatcagcttg atatgcgctg gcgacgtcgt gttccggtta ttcatcagac ggagaccgct
     3661 gaatgtggac tggcctgcct ggcaatgata tgcggtcatt ttggtaagaa tattgacctg
     3721 atatctcttc gccggaagtt taatctctcg gcccgtggag caaaccttgc aggaatcaat
     3781 ggaatagcgg agcagctggg gatggtcacc cgggctcttt cactggagct ggatgaactt
     3841 ggtgccctca aaatgccgtg tattctccac tgggatttca gtcacttcgt cgtgctggtc
     3901 agcgtaaagc gtaaccgtta tgtactgcat gatccggcca gaggcagaag atatctcggt
     3961 cgggaggaaa tgagccggta ttttacgggc attgcacttg aggtctggcc cggaagtgaa
     4021 ttcctggcgg aaacccagca gacccgcata agtctccgtt cactgattaa cagtatttac
     4081 ggtattaaaa gaacactggc gaaaattttc tgtctgtcag ttgtaattga agcaatcaat
     4141 ctggtaatgc cggtgggaac tcagctggtt atggatcatg cgattccggc gggggacaga
     4201 gggctgctga cgcttatttc tgctggcctg atgttcttta tattgctcag ggccgcggtg
     4261 agtatgctgc gtgcatggtc ctcactggtt atgagcacgc tcatcaatat acagtggcag
     4321 tcgggtctgt ttaaccatct tctcagactg ccgctggcgt tttttgaacg ccgtaaatta
     4381 ggtgatatcc agtcgcgttt tggctccctt gacactttga gggccacctt taccacctgt
     4441 gttgttgggg caatcatgga cagtattatg gttgtgggtg tttttgtgat gatgctgtta
     4501 tatggaggat atcttacctg gatagtgctc ggttttacca tggtttacgt tcttattcgt
     4561 ctggtgacat acggctatta ccggcaaata tcggaagaaa ctcttgtcag ggggcccggg
     4621 ccagctccta ttttatggaa agcctgtatg gtattgccac ggtaaaaatc cagggtatgg
     4681 ctgggattcg gggaacacac tggcttaacc tgaaaataga tgcgatcaat tcaggtatta
     4741 agttaaccag gatggatttg ctcttcgggg ggataaatac ttttgttgcc gcctgtgatc
     4801 aggtggcgat tttatggctg ggtgcaagcc ttgtgatcga taatcagatg acaataggaa
     4861 tgtttgtggc atttggttct tttcgtgggc agttttcgga tcgggttgct tcgctgacca
     4921 gttttcttct tcaactgaga ataatgagtc tgcataatga gctcattgca gatattgcac
     4981 tacatgaaaa ggaagaaaag aaaccggaaa ttgaaatcgt tgctgacatg agcccggttt
     5041 cactggaaac cactgattta agctaccggt atgacagcca gtcagcacag gtattcagtg
     5101 gtctgaattt gtctgtggct ccgggagaaa gtgtggctat aactggtgcc tccggtgccg
     5161 gaaaaaccac attaatgaaa gtattatgtg gactgtttga accagatagt ggaaaagtac
     5221 tggttaatgg cacggatata cgtcaacttg gaataaataa ttatcaccgt atgatagcct
     5281 gtgttatgca ggacgaccga ctattttcag gatcaattcg tgaaaatatc tgtgggtttg
     5341 cagaagaaac agacgacgaa tggatgacag aatgtgccaa agcaagtcat attcatgatg
     5401 tgataatgaa aatgccaatg gggtatgaaa cgttaatagg tgaactgggg gaaggtcttt
     5461 ccggcggtca aaaacagcgt atattcattg cccgagcttt ataccggaaa cctggaatat
     5521 tatttatgga tgaggctaca agttctcttg atacagaaag tgaacgtttc gtgaatgctg
     5581 ctataaaaaa gatgaatatc acccgggtga ttattgcaca tagagagaca actttaagaa
     5641 cagttgatcg gatttttaga attacaaatt aggataatat atcactaaaa gcagcattgc
     5701 tgcataataa gctatgctgc tttagaaatt aatcaacttc cactactaga agaggatgtt
     5761 gatgagttaa atcctgagtt taataaacct ccattgtagt ttggattaaa acttccaagt
     5821 cctatactcg gattatagtt aataccgttt gatggtattg ttattgtcct atttgaacca
     5881 tcagcaataa ctcctccaat atatcctcca attgcacttc ctgctatatc cgcactaggg
     5941 atttttgttg ctttacctaa aaaaccacca gccgcagtac ctattgcaac ggatgcatct
     6001 ttatctaatc ctgcaccatg tattaaagca agttcatcgg ttgttatatt tctcatgtta
     6061 tttccttttt ttattaaaga aagataaaac aaataaaaac agtacactcg caagtaacga
     6121 aagaattatt ttctttatga aaagataaag ggttaatgtg tctaagtctg aatttataag
     6181 ttgtattata aagaacaacg agaaataaaa aaata
//
LOCUS       DAZGQL010000174         6204 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.175, whole genome shotgun sequence.
ACCESSION   DAZGQL010000174 DAZGQL010000000
VERSION     DAZGQL010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6204
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.175"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            225..2072
                     /gene="ldtD"
                     /locus_tag="ACHKIR_004857"
     CDS             225..2072
                     /gene="ldtD"
                     /locus_tag="ACHKIR_004857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFO9992484.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALSTPPLSVINQWQLALDKGQLPTFVVGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTIETAETKPMDKQTTSRSKPAPVVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2253..2801
                     /locus_tag="ACHKIR_004858"
     CDS             2253..2801
                     /locus_tag="ACHKIR_004858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HFO9992485.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2828..3475
                     /gene="gloC"
                     /locus_tag="ACHKIR_004859"
     CDS             2828..3475
                     /gene="gloC"
                     /locus_tag="ACHKIR_004859"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HFO9992486.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDA
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVALQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3526..4716)
                     /gene="aspC"
                     /locus_tag="ACHKIR_004860"
     CDS             complement(3526..4716)
                     /gene="aspC"
                     /locus_tag="ACHKIR_004860"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HFO9992487.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETIDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(4901..5989)
                     /gene="ompF"
                     /locus_tag="ACHKIR_004861"
     CDS             complement(4901..5989)
                     /gene="ompF"
                     /locus_tag="ACHKIR_004861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HFO9992488.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNISGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
BASE COUNT         1568 a         1520 c         1575 g         1541 t
ORIGIN      
        1 gaaaactccg tttctgcact aaaaaagtgg cacattacgg cgcgcttttc tttaaacttc
       61 tttacattag gttatgtaaa aacgtatcgg cgtttatata ctgaagataa gcctgatgag
      121 taacaggctt gctcgtcata ctttcgtgag tattggcgtt gtacaggcaa gtcgtaaaat
      181 aacagcctgg ctattcagag tatgataaaa acagggggca agggatgttg cttaatatga
      241 tgtgtggtcg tcggctgtcg gcaatcagtt tgtgcctggc cgtaacattc gctccactgt
      301 tcaatgcgca ggccgatgag cctgaagtaa tccctggcga cagcccggtg gctgtcagtg
      361 aacagggcga ggcactgccg caggcgcaag ccacggcaat aatggcgggg atccagccat
      421 tgcctgaagg tgcggctgaa aaagcccgca cgcaaatcga atctcaatta cccgcaggtt
      481 ataagccggt ttatcttaac cagcttcaat tgttgtatgc cgcacgcgat atgcaaccca
      541 tgtgggaaaa ccgtgatgct gttaaagcct tccagcaaca gctggcagag gtggcgattg
      601 ccggtttcca gccgcagttt aataaatggg tagagttact gaccgatcct ggtgttaacg
      661 ggatggcacg cgacgtggtg ctctctgatg cgatgatggg ctatctccat ttcattgcaa
      721 atattccggt caaaggcact cgctggctat atagcagtaa accttatgcg ctttcaacgc
      781 cgccgctttc ggtgattaac caatggcagc tggcgctgga taaaggtcaa ttgcctacgt
      841 ttgttgtagg attggcaccg cagcatccgc aatatgcggc gatgcatgaa tcgttactgg
      901 ccttactcag tgacaccaaa ccgtggcccc aactgaccgg caaagcaacg ttgcgcccag
      961 gacagtggag taacgacgta ccggcgttgc gcgaaatatt gcaacgtaca ggcatgttgg
     1021 acgggggacc gaaaattacg ctacctggcg atgacacgcc aactgacgcg gtagtcagcc
     1081 catccgctgt tactattgaa acagcagaaa ctaagccgat ggacaagcaa actacgtctc
     1141 gtagtaaacc tgcgcctgtc gttcgtgccg cctacgataa tgaactggtg gaagccgtta
     1201 aacgttttca ggcatggcaa ggattggggg cagatggtgc tattggcccg gcaacgcgtg
     1261 actggttaaa cgtaacgccc gcccagcgtg ctggcgtgtt ggcactcaac atccagcgat
     1321 tgcgcttgct gccaacagag ctttctaccg ggatcatggt taacattcca gcctattcgc
     1381 tggtctacta tcagaacggc aatcaggtgc tggattcgcg agtcattgtc ggtcgccccg
     1441 atcgcaaaac gccgatgatg agcagtgcac ttaacaacgt agtggtaaac ccgccgtgga
     1501 acgtaccacc aactctggca cgcaaagata ttctgccgaa agtgcgcaac gatccgggat
     1561 atctcgaaag ccatggctat acggtgatgc gcggctggaa cagcagagaa gcgattgacc
     1621 catggcaggt tgactggtct acaatcacgg cctcgaattt accgttccgc ttccagcagg
     1681 ctccaggccc acggaactcg ctggggcgct ataaattcaa tatgccgagt tcagaggcca
     1741 tttatttgca tgacacgccg aaccacaatc tgttcaagcg tgatacacgc gcattgagct
     1801 caggctgtgt acgagtgaat aaagcttccg atctggcgaa tatgctgttg caggatgcag
     1861 gctggaatga caaacgtatt tctgatgcgc tgaagcaggg cgatacgcgt tacgtcaata
     1921 ttcggcagtc gattccggtg aatctctact atctgacggc ctttgttggt gcagatggtc
     1981 gtacccagta tcgtacagat atttacaatt atgatctgcc tgcgcgatcc agctcgcaaa
     2041 ttgtatcgaa agcggaacaa ttaatcaggt aaatgaagca gttcggggaa atcagttgtc
     2101 gtaataatta gcatgattgg gggcaattct ctgcagcccc cgtcactgct ggggttgagt
     2161 catcttgacg tctgctttac gggcggttaa ggtgcctctt gtgcgccaga agtgcatata
     2221 aacgataaca ttgacctgta gacttgatta tcatggacaa attcgacgct aatcgccgca
     2281 aattgctggc gcttggtggc gttgcactcg gtgccgccat cctgccgacc cctgcgtttg
     2341 caacactctc taccccacgc ccgcgcattt tgacactcaa taatcttcat accggagagt
     2401 caatcaaagc ggagtttttc gatggcagag gctatattca ggaagaattg gcaaaactta
     2461 accatttttt ccgcgattac cgcgcgaaca aaataaagtc catcgaccca ggattattcg
     2521 accagttgta tcgcctgcaa gggttgttag gtacccgcaa accggtgcaa ctcatttccg
     2581 gttatcgttc cattgatacc aacaatgaac tacgcgcccg cagccgtgga gtagcgaaga
     2641 aaagctatca cacgaaaggg caggcgatgg atttccatat tgaaggtatc gcgttaagca
     2701 atattcgcaa agccgcgtta tctatgcgcg caggtggtgt aggatattac ccacgtagta
     2761 actttgtgca tattgatacc gggccagcac ggcactggta gcaattgctt aacgaaacag
     2821 gggcagtatg aactatcgta ttattccggt caccgcattc tcccagaact gttcattgat
     2881 ctggtgtgaa caaacccgcc tggccgcact ggtcgatcct ggcggcgatg cggaaaaaat
     2941 caaacaggaa gttgacgcca gcggcctgac actgatgcag atcctgctga cgcatggtca
     3001 tctggaccac gttggtgcag cggcggaact ggcgcaacat tacggcgtgc cggtttttgg
     3061 cccggaaaaa gaagatgagt tctggctgca aggcttgcct gcgcaaagtc gtatgtttgg
     3121 tctggaagag tgccagccgc tgacgccgga tcgttggctg aacgaaggcg ataccatcag
     3181 catagggaat gtggctttac aggtgttaca ttgccctggg catacgccgg gtcatgtcgt
     3241 gttttttgat gatcgggcaa agctgctgat ttctggcgac gttattttca aaggcggagt
     3301 agggcgcagt gacttcccgc gtggcgatca taatcaactg atttcttcaa tcaaagataa
     3361 gttgctgcca ctgggggatg acgtgacatt tattccgggt cacggaccat tatccacact
     3421 tggttatgag cgcctgcata atcccttcct gcaagacgaa atgcccgtct ggtaaaggcg
     3481 cataaaaaag cccgcttttt aagcgggctt cattgttttt aacgcttaca gcactgccac
     3541 aatcgcttcg cacagcggag ccatattatc tggtgtcatc ccggccacgt ttacgcgacc
     3601 agaagcaaca gcatatacgc caaactcttc gcgcagacgc agcacttgtt cttttgtcag
     3661 gccactgaag gagaacatgc cgttctgttt gatgataaag ctgaagtcgc gatttgcgcc
     3721 tttttcctgc agcgtattga cgaacaactg acgcatacgc tgaatacgct ggcgcatatc
     3781 agtcagttct tgttcccaaa tcgcacgtaa cgcatcgttg ctcaggatgg tggcaacaac
     3841 agaagcgccg tgtgctggtg ggttagagta gttagcgcga atcgccgctt tcatttggct
     3901 gaatgcgcga tcaatggttt cactgtcagc agcaaccaga gtacaagcgc caacacgctc
     3961 gttgtacagg ccaaagtttt tagagtagga actggcaaca atcagctctt tatgcatagc
     4021 cgcgaaagcg cgcagtcctt cagcatcttc ttccagacca cgggcaaacc cctggtaagc
     4081 gaagtcaaac agcggtaacc agcctttctc aacggaaagt tgtgccagtg tctgccattg
     4141 ttccagcgta gggtcgatac cggttgggtt atggcagcag ccatggaaca gcaccacgtc
     4201 gccagcctga gcttcgttca ggctgttaat cagtgcatcg aagtcaaggg tgtgattttc
     4261 cgcatcataa taagcgtatt cacgaacttc cagacctgca gagttaaaga cgctcttatg
     4321 gttcggccag cttgggttgc tcacccacac acgcttaacg ctggtatttt ttgccaggaa
     4381 atcggcagcc acgcgtagtg cgccagtgcc acccggagtc tgtgccgtgc gagcacgttt
     4441 gtcattgatc agggcgctac ctttaccaaa cagcagttcc tgagtgcagc gaccaaattc
     4501 agggatgccg tcaatgccga ggtaattttt ggtggtttca ttttcgagca gatactgttc
     4561 agcctttttc acactggtca gtaccggggt tttgcccgtc tcatctttat agacaccaat
     4621 cccgaggtta attttgccgg gacgttcatc ggcacgaaac agatcggcca ggcccagaat
     4681 cgggtcggca ggagcggcgg taatgttctc aaacatgacg aggttccatt atggttacag
     4741 aagggaagtc cgctatcagg gtaacgggag atttacaaaa ttccaactat tactgatgaa
     4801 aacgcaggct gtttttgcaa gacgtgagat tgctctggaa ggcataaaaa aaacaggacc
     4861 aaagtcctgt tttttcggca tttaacaaag aggtgtgcta ttagaactgg taaacgatac
     4921 ccacagcaac ggtgtcgtca gaacctacgc ccagtttgtt gtcagaatcg atctggttga
     4981 tgatgtagtc aacataggtg gacatgtttt tgttgaagta gtaggttgcg cccacttcaa
     5041 agtagttaac cagatcaaca tcaccgatac cttctacgtc tttcgcttta gatttggtgt
     5101 aagcgatgga cggacgcaga ccgaaatcga actggtattg cgcaactaac agaacgtctt
     5161 gcgttttgtt ggcgaagccg ctgatgtttg taaatttatt agtgatcggc gtagcgttac
     5221 gggtttcacc gtagttcgct gccaggtaga tgttgttcgc gtcatacttc agaccagtag
     5281 cccactgttc agctttttta ccgttgccaa gaggttgagc ttcttgcagg ttggtacggt
     5341 cagcggcgcc ataagcacca acgataccaa agccttcgta ttcgtagctg atagaaccgc
     5401 caacaccgtc gccgttagag cggcgtgcag tgtcacgctc gtttttaccc aggtactgaa
     5461 cagcgaagtt caggccatca accagaccaa agaagttgga gttacgatag gtagcaacgc
     5521 cgccaacacg accaacgaag aagtcatcgc tgtatgccgt gtcaccgcca aactcaggca
     5581 gcatatcggt gtaacccagt gcatcataaa ccacaccgta gttacggccg taatcgaaag
     5641 aaccaacgtc agcgtattta agacccgcga atgccagacg cgttttgtta ccagtttgag
     5701 cgtcagcgcc ttcagagttg ttaccctgga agttatattc ccactgacca taaccggtca
     5761 gatcggaatt gatttgagtt tcccctttaa aaccaagacg ggcataggtc atgtcaccat
     5821 tgccaccgta actgttttca ccgttaccct tggagaaata atgcagaccg acagctttac
     5881 cgtacagatc tactttgttg ccatctttgt tatagatttc tgcagcgttt gcagtacctg
     5941 ctactaacag agcagggacg atcactgcca gaatattgcg cttcatcatt atttattacc
     6001 ctcatggttt ttttatgaca cctgccactg ccgtcaataa gttctgtcaa taaaaattta
     6061 cggaactatt gatgagagtt tggtgtcttt atgtgtctgc aggcatcttt ccattcaaac
     6121 taacgtttcg ctaccgtgaa agtgctacaa agataaagat ttggtttcaa aaagaaaaaa
     6181 tatgtaacca aaagtaaaat ttaa
//
LOCUS       DAZGQL010000175         6080 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.176, whole genome shotgun sequence.
ACCESSION   DAZGQL010000175 DAZGQL010000000
VERSION     DAZGQL010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6080
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.176"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            218..1348
                     /gene="ompC"
                     /locus_tag="ACHKIR_004862"
     CDS             218..1348
                     /gene="ompC"
                     /locus_tag="ACHKIR_004862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFO9992489.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFGL
                     VDGLNFAVQYQGKNGSVDGEGMTNNGRGALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDAQNGTYVSDNNVVRNYIGTGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSL
                     GWANKAQNFEAVAQYQFDFGLRPSLAYLQSKGKNLGVINGRNYDDEDILKYVDVGATY
                     YFNKNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            1460..2515
                     /gene="apbE"
                     /locus_tag="ACHKIR_004863"
     CDS             1460..2515
                     /gene="apbE"
                     /locus_tag="ACHKIR_004863"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HFO9992490.1"
                     /translation="MEISFTRVALLAAALFFVGCDQQPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGAKTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGAGKPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDNF
                     KTWMSPQFKSFLISEKN"
     gene            2589..3653
                     /gene="ada"
                     /locus_tag="ACHKIR_004864"
     CDS             2589..3653
                     /gene="ada"
                     /locus_tag="ACHKIR_004864"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HFO9992491.1"
                     /translation="MKYATCLTDDQRWQSVLARDSNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPLQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRELLAKGESVTTSIINAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADSAPADLTFQQHVRDVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            3653..4303
                     /gene="alkB"
                     /locus_tag="ACHKIR_004865"
     CDS             3653..4303
                     /gene="alkB"
                     /locus_tag="ACHKIR_004865"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HFO9992492.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILHRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHHLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            4379..6022
                     /gene="yojI"
                     /locus_tag="ACHKIR_004866"
     CDS             4379..6022
                     /gene="yojI"
                     /locus_tag="ACHKIR_004866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HFO9992493.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAVT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFSYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQNGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
BASE COUNT         1446 a         1532 c         1637 g         1465 t
ORIGIN      
        1 ttggtcattc cgcaataatc ttaaaaagtt ccctttcatt tacattctga aacatctata
       61 gcgataaatg aaacatctta aaagttttag tatcatattc gtgttggatt attctgcatt
      121 tttggggaga atggacttgc cgactgatta atgagggtta atcagtatgc agtggcataa
      181 aaaagcaaat aaaggcatat aacagagggt taataacatg aaagttaaag tactgtccct
      241 cctggtccca gctctgctgg tagcaggcgc agcaaacgct gctgaagttt acaacaaaga
      301 cggcaacaaa ttagatctgt acggtaaagt agacggcctg cactatttct ctgacgacaa
      361 gtctgtagat ggcgaccaga cctacatgcg tcttggcttc aaaggtgaaa ctcaggttac
      421 tgaccaactg accggttacg gccagtggga atatcagatc cagggcaaca gcgctgaaaa
      481 cgaaaacaac tcctggaccc gtgtggcatt cgcaggtctg aaattccagg atgtgggttc
      541 tttcgactac ggtcgtaact acggcgttgt ttatgacgta acttcctgga ccgacgtcct
      601 gccagaattc ggtggcgaca cttacggttc tgacaacttc atgcagcagc gtggtaacgg
      661 cttcgcgacc taccgtaaca ccgacttctt cggtctggtt gacggcctga actttgctgt
      721 tcagtaccag ggtaaaaacg gtagcgtaga tggcgaaggc atgaccaaca acggtcgtgg
      781 tgctctgcgt cagaacggcg acggcgttgg cggttctatc acttatgatt acgaaggctt
      841 cggtatcggt gctgcagtgt ccagctccaa acgtactgac gctcagaatg gtacctatgt
      901 aagcgacaat aatgtggtcc gtaactacat cggtactggt gaccgtgctg aaacctacac
      961 tggtggtctg aaatacgacg ctaacaacat ctacctggct gctcagtaca cccagaccta
     1021 caacgcaact cgcgtaggtt ccctgggttg ggcgaacaaa gcacagaact tcgaagctgt
     1081 tgctcagtac cagttcgact tcggtctgcg tccgtccctg gcttacctgc agtctaaagg
     1141 taaaaacctg ggtgtcatca atggtcgtaa ctacgacgac gaagatatcc tgaaatatgt
     1201 tgatgttggt gcgacctact acttcaacaa aaacatgtcc acctatgttg actacaaaat
     1261 caacctgctg gacgacaacc agttcactcg tgacgctggc atcaacactg ataacatcgt
     1321 agctctgggt ctggtttacc agttctaatc tcgattgata tcgaacaaag ggcctgcggg
     1381 cccttttttc attgttttca gcgtacaaac acagtttttt ggtgtactct tgcgaccgtt
     1441 cgcatgagga taatcacgta tggaaataag ctttacccgc gtggcactgc tggctgccgc
     1501 gctcttcttt gttggttgcg atcaacaacc acaacccgcc aaaacccacg ctactgaagt
     1561 taccgttctt gaaggcaaaa ccatgggtac cttctggcgt gccagcatcc cgggcattga
     1621 cgccaaacgc agtgccgaac ttaaagaaaa gattcagacc caactggacg ctgacgatca
     1681 gctgctttcg acctataaaa aagattccgc gctgatgcgc tttaacgact cgcaaagttt
     1741 gtcgccgtgg ccggtaagtg aagcgatggc cgatatcgtc actacctcgc tgcgcattgg
     1801 cgcgaagacc gatggcgcga tggatataac cgtcgggccg ctggtgaatc tgtggggctt
     1861 tggtccggaa caacaaccgg ttcaaattcc gagccaggaa cagatcgatg cgatgaaagc
     1921 caaaaccggc ttacagcacc tgacggtcat taatcagtcg catcagcaat atctgcaaaa
     1981 agacctgccg gatttatatg tcgatctctc caccgtcggc gaaggttatg cggcggatca
     2041 cctggcacgc ttgatggagc aggaagggat ttcccgctat ctggtgtcgg tgggcggcgc
     2101 gctgaacagc cgtggtatga acggtgctgg caagccgtgg cgggtagcga ttcaaaagcc
     2161 aaccgataaa gaaaacgcgg ttcaggccgt ggtggatatc aacggtcatg ggattagtac
     2221 ctctggcagc taccgtaact attacgaact ggacggcaaa cgtctttctc atgttatcga
     2281 tccacaaacc gggcgtccta tcgaacacaa tctggtatcc gtgacggtga ttgccccgac
     2341 ggctctggaa gccgatgcct gggatactgg cttaatggta ctcggaccgg agaaagccaa
     2401 agaggttgtt cgccgggaag gactggcggt ctacatgatc accaaagagg gcgataattt
     2461 taaaacctgg atgtcgccgc agtttaaaag cttccttatc agcgaaaaaa attaaagcgc
     2521 aagattgttg gtttttgtgc gatggtgacc gggcagacta aaggctatcc ttaaccaggg
     2581 agctgattat gaaatatgcc acatgcttaa ctgacgatca acgctggcaa tctgtcttag
     2641 cccgcgactc gaatgccgac ggcgaattcg ttttcgccgt gcgcaccacg ggtatctttt
     2701 gccgtccgtc ttgccgagcc agacatgcct tgcgggaaaa tgtctccttc tacgcaaatg
     2761 ccagcgaggc gcttgccgct ggctttcgtc cctgcaaacg ttgtcagcca gacaaagcca
     2821 atcccctgca acatcgcctt gataaaatta cccacgcctg ccgacttctg gaacaggaaa
     2881 cgcctgtaac gctggaagcc ttagccgacc aggtggcgat gagtccattt catttgcatc
     2941 ggttgtttaa agcgactacc ggaatgacgc ctaaagcctg gcaacaggcc tggcgcgctc
     3001 gccgtttgcg cgaattgctg gcgaaagggg agagcgtgac tacatctatt attaacgccg
     3061 gattccccga cagcagcagt tactaccgca aagccgacga aacgctgggc atgacggcta
     3121 aacaattccg tcacggtggc gaaaatctgg cggtgcgtta cgcgctggct gattgtgagc
     3181 tgggccgttg cctggtggca gaaagtgagc gggggatttg cgcgatatta ctgggcgatg
     3241 atgacgcgac actaatcagc gagttacagc agatgtttcc cgctgccgac agcgcgcctg
     3301 ctgatcttac gtttcagcaa catgtgcgtg acgtgatcgc cagcctcaat caacgcgata
     3361 cgccgctgac gttaccgctg gacattcgcg gcactgcttt tcagcaacaa gtctggcagg
     3421 cactgcgcac gataccttgc ggtgaaaccg tcagttatca gcaactggct aatgccatcg
     3481 gcaaaccgaa agcggtacgg gccgttgcca gcgcctgtgc cgccaacaag ctggctatcg
     3541 taataccctg tcatcgggtg gtccgtggtg atggcacact ttccggttac cgctggggcg
     3601 tgtcgcgcaa agcgcaactg ctgcgccgcg aagctgaaaa tgaggaaagg taatgttgga
     3661 tctgtttgcc gatgctgaac cgtggcaaga accactggcg gctggagcgg tgattttgca
     3721 ccgttttgct tttaacgctg cggagcaact gatccgcgat attaatgacg ttgccagcca
     3781 gtcgccgttt cgccagatgg tcactccagg cggatatacc atgtcggtgg cgatgaccaa
     3841 ctgtgggcat ctgggctgga cgacccatcg gcaaggttat ctctattcgc ccattgatcc
     3901 gcaaacaaat aaaccgtggc ccgccatgcc gcagagtttt catcatttat gtcaacgtgc
     3961 ggctacggcg gcgggctatc cagatttcca gccagatgcc tgtttgatca accgctacgc
     4021 tcctggcgcg aaactgtcgc tgcatcagga taaagacgaa ccggatctgc gcgcgccgat
     4081 tgtttctgtt tctctgggct tacccgcgat ttttcaattt ggcggcctga aacgaaatga
     4141 tccgctaaaa agattgttgc tggaacatgg cgatgtggtg gtatggggcg gtgaatcgcg
     4201 gctgttttat cacggtattc aaccgttgaa agcggggttt catccactca ccaccgactg
     4261 ccgctacaac ctgacattcc gtcaggcagg taaaaaagaa taaaaataag aattattatt
     4321 gctgtgcgcg cgaagatgtt taaactgcgg gctgtaattc attgtccggg ttttctgcat
     4381 ggaacttctt gtacttgtct ggcggcagta tcgctggcca tttatcagtg tgatggcgct
     4441 aagcctcgcc agtgcggcat taggcattgg cttaattgct tttatcaatc agcgccttat
     4501 cgaaacggcg gataccagtc tgctggtatt gccggagttt ctggggttat tgctgctgtt
     4561 gatggccgtc actctcggat cgcaactggc gctcaccact ttggggcatc acttcgttta
     4621 ccgactgcgt agtgaattta tcaagcggat tctcgatact cacgtcgagc gcattgaaca
     4681 actcggtagc gcctcgttgc tggcgggatt aaccagcgat gtgcgcaata tcaccattgc
     4741 ttttgtgcgt ctgccggaac tggtgcaggg gatcattctc actatcggtt cagcggcgta
     4801 tttgtggatg ctgtcgggca aaatgttgct ggtaacggct atctggatgg cagtcaccat
     4861 ctggggcggt tttgtgctgg tggcgcgggt gtacaaacat atggcgaccc tgcgtgaaac
     4921 cgaagataag ctgtacacgg attttcaaac tgtactcgaa gggcgcaaag agctgactct
     4981 gaaccgtgaa cgcgccgagt atgtgtttaa caacctctac attcctgatg cgcaagagta
     5041 tcgccatcat attattcgcg cagacacctt ccatcttagt gccgtgaact ggtcaaacat
     5101 catgatgctg ggcgctatcg gcctggtgtt ctggatggcg aacagtctcg gttgggctga
     5161 taccaacgtt gctgcgacct attcgttgac gctgttattc ctgcgtacgc cgctgctttc
     5221 ggcggttggc gcattgccga cgctgctgac ggcgcaggtg gcgtttaaca agctgaacaa
     5281 attcgcgctc gcgcctttca aagcagagtt tccgcgcccg caggcttttc ccaactggca
     5341 aacgctggag ctgcgtaacg tgacgtttag ctatcaggat aacgcgtttt ccgttgggcc
     5401 aattaacctc accatcaaac gtggcgagct gctgtttctg attggtggca acggtagtgg
     5461 aaaatcgacg ctggcgatgt tgctgacggg cttgtaccag ccacaaaatg gcgaaatctt
     5521 gctggatggc aaacctgtca gcggcgaaca accggaagat tatcgaaaac tgttttcggc
     5581 agtgtttacc gatgtctggc tgtttgatca actgctgggg ccggaggggc aacccgctaa
     5641 cccgcaactg gttgagaagt ggctggcgca gctgaaaatg gctcataagc ttgagttaag
     5701 caacgggcgt attgttaacc tgaagttatc aaaagggcag aaaaaacgcg tggcgctgtt
     5761 gctggcgctg gcagaagaac gcgatattat cctgctggat gaatgggcgg cggatcagga
     5821 tccacacttc cgtcgtgagt tttatcaggt gctgctgccg ctgatgcagg agatgggtaa
     5881 aactattttc gccatcagtc atgatgatca ttactttatc cacgccgacc gcctgctgga
     5941 aatgcgcaac gggcaactta gcgagctgac aggcgaagag cgcgatgctg cttcgcgtga
     6001 tgccgttgcc cggacggcat aatattacga cgctgacctg gcggactgct gccgccaggt
     6061 taatatgcta aaaatccctt
//
LOCUS       DAZGQL010000176         6034 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.177, whole genome shotgun sequence.
ACCESSION   DAZGQL010000176 DAZGQL010000000
VERSION     DAZGQL010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6034
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.177"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            289..870
                     /gene="mdaB"
                     /locus_tag="ACHKIR_004867"
     CDS             289..870
                     /gene="mdaB"
                     /locus_tag="ACHKIR_004867"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HFO9992494.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     ESDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            901..1215
                     /gene="ygiN"
                     /locus_tag="ACHKIR_004868"
     CDS             901..1215
                     /gene="ygiN"
                     /locus_tag="ACHKIR_004868"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HFO9992495.1"
                     /translation="MITVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GL"
     gene            complement(1260..2147)
                     /locus_tag="ACHKIR_004869"
     CDS             complement(1260..2147)
                     /locus_tag="ACHKIR_004869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFO9992496.1"
                     /translation="MIQKDKVRVDIFGERFRTRASQLTPGLRVVASYINEHREVVLEQ
                     TAMEIAATLNTSDATVIRAIQALGFAGLRDLKRTLEQWLGPALSSSEKMSTTVSNLTS
                     DVNTAIDFVLEGHLYTCNVLSEPENRHALAQAVALLVQARQVAIFGIGASGILADYTA
                     RLFNRIGLPATALNRTGIGLAEQLIALQRGDVLIMMAQKSAHREGLTMLREARRLGIP
                     VILLTNALDSRFSKDASIVIHVPRGDEKGKTPLHGTVLLCLEMIVWSVASAVPQRAVK
                     TIKRINDFHRGLKTGRKNG"
     gene            complement(2144..3091)
                     /locus_tag="ACHKIR_004870"
     CDS             complement(2144..3091)
                     /locus_tag="ACHKIR_004870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-siderophore ABC transporter
                     substrate-binding protein"
                     /protein_id="HFO9992497.1"
                     /translation="MRLFFSLLILLSFFSRATEPVQVFTDDLGRKVTVPAHPKRIVSL
                     HDLDITIPLIELGVPPVASHGRTRPDGSHFIRSGALLTGVDFDNSSIAFIGTADIDIE
                     AIVAAKPDLIITEPTRNTPIEQLEKIAPTVSIDHLKGGAPEIYRKLAGLTGTQSQLAI
                     LERRYQAQINALKATLDSQKITVSVIQANQGKINVMHSYHSLGRVLRDAGFRFPPLIE
                     SIPEGGRMDVSAERLPELDADFVFATWRGDTGGKPQDELAAMEKVMPGWCQFLTACRS
                     GRYVLISREEAISNSFASLGLMAAQIQSQIAGRPLPEAK"
     gene            complement(3102..4139)
                     /locus_tag="ACHKIR_004871"
     CDS             complement(3102..4139)
                     /locus_tag="ACHKIR_004871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFO9992498.1"
                     /translation="MNVGLRPLRVGKFSTLVRPKNLVLLGGLFLFAVGILIFGLMHGS
                     FSVPASEVGRALFAPENVSTDARYIVQDIRLPRVIMALLCGAMLGMAGAAMQSIARNG
                     LADPGLIGVKEGCSVAVLWLIFQFPMLGMFWRPVAGLAGGLLVALIVIFCARDISRPR
                     FVLIGIGVSWFFAAGIGVFMTTADVRDVQTALMWLSGSLHAANWMLVGISACWMLPAA
                     LLLLFTARTADIALLGHQVATGLGVNSSRLALLRVAAPIILTAVCVSCVGNIGFVGLI
                     APHISRFILRGGQTTLLLGSAVSGALLVILADSIGRLAFLPLQLPAGIIISLIGGPFF
                     LLLLWQRRNSF"
     gene            complement(4148..5131)
                     /locus_tag="ACHKIR_004872"
     CDS             complement(4148..5131)
                     /locus_tag="ACHKIR_004872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFO9992499.1"
                     /translation="MMRTGLVTVIFLALLLVGCVVYPGIGARFIAPQTVLQAFLHFDP
                     QNFDHNVIVRLRLPRLAAALLTGASLGVAGALLQAVIRNPLGEPHILGLNAGAALAVV
                     AASALGLAFPVGRPLLASTGGALLFLLILLLSSAGRSGLTPMKVTLCGVALSAFVSSI
                     TAAILIFDEQTLLAMRTWLAGDLAGQDWVTLGTSAWFSLGGFVLAIYLAPSLNMLALG
                     DRMAQGLGVSVLRTRTFTLLAIALLCGAAVSIAGPIGFVGLLVPQIVRRLVSADLRVL
                     LPLSACVGALLLLLADIIARTLFTPHELATGVMTALVGAPVFVIMATRMFK"
     gene            complement(5128..5937)
                     /locus_tag="ACHKIR_004873"
     CDS             complement(5128..5937)
                     /locus_tag="ACHKIR_004873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO9992500.1"
                     /translation="MAHNAKGQGLILDNVSAGYHKKIIVDGVSFSVPTEKMTVLVGAN
                     GCGKSTLLSTIARILQPMGGSILLDGKAIHEQPTKALSRRLGILPQSPLLPEGLTVYE
                     LVSRGRFPWQNFIRQWSDADEQAVEEALKLTGTQEFAHLPVEKLSGGQRQRCWIAMVL
                     AQKTPYILLDEPTTWLDLRYQVEILELLHDLTRHHGRTVVVVLHDLNFAVNYGDTLIF
                     LRQGKVVRVLNEGEHCTPELVKAVFDVDVHASINPLTGKPFFMPFRGVEKV"
BASE COUNT         1713 a         1631 c         1374 g         1316 t
ORIGIN      
        1 gtgtttttcc tttaattgat aagtatatga tatttgacat atatattctc catgattatt
       61 tttccgaaaa actactttat catttacata atacatctct cctgtgggaa cttataggaa
      121 gttgatttca gtattgtcat tttcagaaaa tatttatccc gcatcggtga atcagagcaa
      181 gatcagactt ttgctaaatt cgcaaaagac tttgcacatt ttgctaattt caccgtacca
      241 ctctgtgacg tactatagtc gacaaacgtc tcaccttgag gttaaaaaat gagcaacatc
      301 ctgattatca acggcgcgaa aaaattcgcc cactccaatg gtcaactgaa cgacaccctg
      361 accgaagtcg cggatggcac actgcgcgac ctcgggcatg atgtccgcat cgttcgcgcc
      421 gagagcgact acgatgtcaa agccgaagta caaaactttc tctgggctga tgtggtgatc
      481 tggcagatgc ctggctggtg gatgggcgcg ccgtggacag tgaaaaaata cattgatgat
      541 gtattcaccg aaggtcacgg gactctgtat gccagcgatg gtcgtacccg caaagatccg
      601 tcgaaaaaat acggttctgg cggtctggta caaggtaaaa aatatatgct ttccctgacc
      661 tggaacgcac caatggaagc tttcaccgaa aaagatcagt tcttccacgg ggttggcgtt
      721 gacggtgtgt atctgccgtt ccataaagca aaccaattcc ttggtatgga accgctgccg
      781 acatttatcg ctaatgacgt gataaaaatg cctgatgttc cccgctatac tgaagaatat
      841 cgcaagcatc ttgtggaaat ttttggttaa ctagagctct ggctttagaa ggagttaacc
      901 atgattacag taatcgcaga aatccgtact cgtcctggtc aacatcaccg tcaggcagtg
      961 ttggatcagt ttgctaaaat cgttccaacc gtactgaaag aagaaggttg ccacggctat
     1021 gcgccaatgg tggattgcgc tgctggcgtg agtttccagt ctatggcacc ggattctatc
     1081 gtgatgattg agcagtggga aagcatcgcg catcttgaag cgcatctgca aaccccgcac
     1141 atgaaagcat atagcgaagc cgtaaaaggt gacgtgctgg agatgaatat tcgtatcctg
     1201 cagccggggc tttgacattt ctaaaaagag gccggtgctg ctaccggtct cagcccagat
     1261 tagccattct ttcttcccgt tttcagtcct cgatggaaat cgttaattcg cttaatggtt
     1321 ttaaccgcac gctgcggcac tgcggaagct accgaccaga caatcatttc cagacacaac
     1381 aataccgtac catgtagcgg cgttttccct ttttcatccc cgcgcggaac atgaatcacg
     1441 atactggcgt ctttgctaaa acgtgaatcc agcgcgtttg tcagcaaaat aacagggatc
     1501 cccagccgcc tcgcctcacg aagcattgtc agcccttccc ggtgcgcaga tttttgcgcc
     1561 atcatgatca gaacatcgcc acgttgaagc gcgatcagtt gctcggcaag gccaataccc
     1621 gtacggttta gtgccgtggc aggcaaacct atgcggttga atagtcgtgc ggtatagtcc
     1681 gccagaatac ccgaagcgcc aatgccaaaa atagcgactt gccgtgcctg caccaacaac
     1741 gctacagctt gcgcaagcgc gtggcggttt tcaggctctg acaggacatt acaggtatac
     1801 aaatgccctt caagaacaaa gtcgattgcg gtgttaacat cgctcgtcaa gttactcacc
     1861 gtcgtagaca tcttttcgct ggaactaagg gctggaccaa gccactgttc cagagtgcgt
     1921 ttcagatcac gcagcccggc aaaccccagg gcctgtatag ccctgataac tgtggcatcg
     1981 gaagtgttca acgtggccgc aatttccatc gccgtttgct cgagtaccac ttcacgatgc
     2041 tcattaatgt agctggcgac aactcttaac ccaggcgtta actgactcgc gcgagtgcga
     2101 aaacgctcac caaaaatatc aacccgcact ttatctttct ggatcattta gcctccggta
     2161 gaggtcgccc tgcaatttgt gattgtatct gcgcagccat cagccccaga gatgcaaagg
     2221 agttagaaat tgcctcttca cgagaaatca gcacataacg cccggaacga caggcagtca
     2281 gaaactgaca ccatcccggc atgacttttt ccattgctgc cagctcatcc tgaggtttac
     2341 caccggtgtc accgcgccag gttgcaaaaa caaaatcggc atccagttca ggtaaacgct
     2401 ccgcgctcac atccattcgc ccaccttcag gaatgctctc aatcagcggc ggaaaacgaa
     2461 aacccgcatc gcgtaatacc cgccccagcg aatgatagct atgcatcaca ttaatttttc
     2521 cctggtttgc ctgaatcacc gataccgtta ttttttggct gtccagcgtc gccttcagcg
     2581 cattgatttg tgcctgatag cggcgttcaa gtatcgccag ctgcgactgc gttcctgtaa
     2641 gtcctgccag cttgcgatag atttctggcg cacccccttt gagatgatca atgctcaccg
     2701 tgggcgcaat tttttcaagt tgttcaatgg gcgtattacg ggtcggttct gtgatgatca
     2761 ggtcaggctt tgccgccaca atcgcttcaa tatcaatatc agcggtacca ataaaggcga
     2821 tcgatgaatt atcaaagtcg acacctgtca ataacgcgcc agatcgaatg aaatggctac
     2881 cgtcaggccg agttcgcccg tggctggcga caggaggtac accgagctca atcagaggaa
     2941 tagtgatatc aagatcgtgt aaagagacaa ttctctttgg atgagcagga acagtgactt
     3001 tgcgccctaa atcatcagta aatacctgaa caggttctgt tgcacgggaa aaaaatgaca
     3061 acagtatcag cagagaaaaa aataaacgca tataactccc gttaaaaact gttcctgcgc
     3121 tgccagagca gtagcaaaaa aaatggtcca ccaatcagtg agataatgat tccggcaggc
     3181 agttgcaaag gcaaaaaagc caaacgacca atgctatccg ccagaataac taacaacgcc
     3241 cccgataccg cgctccccag cagtaatgtc gtctgcccgc cacgtagaat aaaacgtgat
     3301 atatggggtg ctatcaatcc gacaaaaccg atgttaccaa cacacgaaac gcagacggct
     3361 gtcaggatta ttggtgcggc gacacgtaac aacgccagac gagaactatt cacccctaac
     3421 ccggttgcaa cctgatgacc aagcagagcg atatccgccg tcctggcggt gaacagcaat
     3481 aaaagagcgg ctggcaacat ccaacaggct gaaataccga ctaacatcca gttagctgca
     3541 tgcaggcttc ccgataacca catcaatgca gtttgtacat ctcgaacatc tgcagtggtc
     3601 ataaatacac cgataccggc ggcgaaaaac caggaaacac caataccaat cagaacaaat
     3661 cgcgggcggg aaatatcgcg cgcacaaaaa atcacgatta aagccaccaa caagccacca
     3721 gcaagaccag ccactggacg ccagaacatt cccagcattg gaaactggaa aatcagccac
     3781 agcaccgcca cgctacatcc ttctttgacg ccgattaaac caggatcggc aaggccgtta
     3841 cgcgcaatgg attgcatcgc ggcccccgcc atacctaaca tggctccgca tagcaatgcc
     3901 atgatgactc tcggcaagcg aatatcctgc acaatataac gtgcgtctgt gcttacgttt
     3961 tctggagcaa acaacgctcg tcctacttcc gatgccggga cagaaaaaga accatgcatc
     4021 agaccgaata tcagtattcc aaccgcaaac agaaacagcc caccaagcaa caccagattt
     4081 ttagggcgta ccagtgtcga aaacttacca acacgtagtg ggcgtagccc gacgttcatt
     4141 atccgactca tttgaacatc ctcgtcgcca taatgacaaa aacaggcgca ccaactaacg
     4201 ccgtcatgac accggtcgct aactcatggg gcgtgaaaag cgtccgagca ataatatctg
     4261 ccagtaataa caaaagcgcg ccgacacaag ctgaaagagg gagcagaacg cgcagatccg
     4321 ctgataccag acgccggaca atctgcggta ctagcaggcc gacaaaacca atggggccag
     4381 caatcgatac ggctgcgcca cagagcaacg cgatcgcaag cagggtgaat gttcgcgtgc
     4441 ggagtaccga gacgcccagc ccctgcgcca tgcgatcgcc cagtgccaac atgttaagag
     4501 agggcgcaag gtaaatggct aacacaaatc cccccagaga aaaccaggcg gacgtaccca
     4561 gcgtcaccca atcctgcccc gctaaatcac ctgctagcca tgtccgcatc gccagcagtg
     4621 tttgttcgtc aaaaatcagg attgcagccg ttattgagga cacgaacgcc gatagcgcca
     4681 cgccgcataa agtaactttc attggcgtta accctgaacg ccctgcggag gaaagcagca
     4741 atatcaacag gaacaataac gcgccccccg tagatgccag cagtgggcgt ccaaccggaa
     4801 aggccagtcc aagcgcactg gcggcaacga cagcaagagc ggctccggca tttaagccta
     4861 aaatatgtgg ctcccccaat gggttacgaa tgacagcctg caacaaagca ccagcaacac
     4921 caagagaagc accggtaagc aatgctgccg ctaaacgcgg caaacggagt cttacaatta
     4981 cgttgtgatc gaagttttgc ggatcaaaat gaagaaatgc ctgcaacacc gtttgtggtg
     5041 caataaatcg ggcaccaatc cctggatata ccacgcatcc caccagcagt aaggcgagga
     5101 aaattacagt aaccagccct gtgcgcatca tactttttct acaccacgaa acggcatgaa
     5161 gaacggcttt cccgtcagag gatttattga tgcgtgaaca tcaacgtcaa agacagcttt
     5221 taccagttca ggcgtgcaat gctccccctc attcaacaca cgcaccactt tcccctggcg
     5281 taaaaatatc aacgtatcgc cataattgac ggcaaaattc agatcatgca acaccacgac
     5341 gactgtacgc ccgtgatgac gcgttaaatc atgtaataac tcaagaattt caacctgata
     5401 acgtaaatca agccaggttg tgggttcatc cagcaggata taaggcgtct tttgcgccaa
     5461 caccatcgca atccagcaac gctggcgctg cccaccagag agtttttcaa caggtaaatg
     5521 tgcaaactcc tgcgtaccgg ttaatttcag agcctcttct accgcctgct cgtcggcgtc
     5581 gctccattga cgaatgaaat tctgccaggg aaaacgccca cgcgacacca gttcataaac
     5641 ggttaagccc tccggcaata atggcgactg cggcaagatc ccaagtctac gggataacgc
     5701 tttcgttggt tgctcgtgaa tcgctttgcc atcaagcaat atcgaaccgc ccataggttg
     5761 caagatacgt gcaatggtgc tgagcaacgt cgactttcca cagccgtttg ccccaaccag
     5821 aacggtcatt ttttctgtgg gaacggagaa agaaacacca tcaacaatga tcttcttgtg
     5881 atacccggca gaaacattgt cgaggatcag cccctgccct tttgcgttat gtgccacgtg
     5941 tacgccaact taatcaaaga caaacaaaaa taaaactcat tctcctttga gagtttgacg
     6001 ccacaaaatg tagtagtcaa gcagttaaaa gcca
//
LOCUS       DAZGQL010000177         5977 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.178, whole genome shotgun sequence.
ACCESSION   DAZGQL010000177 DAZGQL010000000
VERSION     DAZGQL010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5977
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.178"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            126..884
                     /gene="ydiY"
                     /locus_tag="ACHKIR_004874"
     CDS             126..884
                     /gene="ydiY"
                     /locus_tag="ACHKIR_004874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HFO9992501.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            complement(937..1044)
                     /gene="yniD"
                     /locus_tag="ACHKIR_004875"
     CDS             complement(937..1044)
                     /gene="yniD"
                     /locus_tag="ACHKIR_004875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YniD"
                     /protein_id="HFO9992502.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(1211..2241)
                     /locus_tag="ACHKIR_004876"
                     /pseudo
     CDS             complement(1211..2241)
                     /locus_tag="ACHKIR_004876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
     gene            2764..4692
                     /gene="thrS"
                     /locus_tag="ACHKIR_004877"
     CDS             2764..4692
                     /gene="thrS"
                     /locus_tag="ACHKIR_004877"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFO9992503.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSDYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            4696..5238
                     /gene="infC"
                     /locus_tag="ACHKIR_004878"
     CDS             4696..5238
                     /gene="infC"
                     /locus_tag="ACHKIR_004878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFO9992504.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            5335..5532
                     /gene="rpmI"
                     /locus_tag="ACHKIR_004879"
     CDS             5335..5532
                     /gene="rpmI"
                     /locus_tag="ACHKIR_004879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFO9992505.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            5586..5942
                     /gene="rplT"
                     /locus_tag="ACHKIR_004880"
     CDS             5586..5942
                     /gene="rplT"
                     /locus_tag="ACHKIR_004880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFO9992506.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
BASE COUNT         1652 a         1267 c         1448 g         1610 t
ORIGIN      
        1 tacattgcta agttattcgc gcctttttat aattcgttac ctttccaggg acgctaatct
       61 ttgatcctta agaaagtttc cgggaccgcg aaggtctctt tttttgttgt acggactctt
      121 gataaatgaa gcttttgaag acagttcctg ccatcgttat gttggcgggg ggcatgtttg
      181 cgtcactgaa tgcagctgcc gatgattccg tttttactgt catggacgat cctgccagcg
      241 ctaaaaaacc tttcgaaggt aatctgaacg caggatatct tgcacaatca ggcaatacga
      301 aaagctcctc acttacggct gacaccacca tgacctggta tggacaaacc actgcctggt
      361 cgctgtgggg gaacgccagt aatacttctt ctaatgatga gcgttcttca gaaaaatatg
      421 cggcgggcgg gcgtagccgt tttaatttaa ctgattatga ctatttattt gggcaggcaa
      481 gctggctgac agaccgttat aacggctatc gagagcgcga tgtgttgacc gcgggttatg
      541 gtcgccagtt tttgaatggc ccggtacaca gtttccgctt tgaattcggt ccaggtgtgc
      601 gttacgacaa atataccgat aatgccagtg aaacccagcc gctgggttat gcctccgggg
      661 cttatgcatg gcagttgact gacaacgcta aatttacgca gggtgtttcc gtctttggcg
      721 cggaagacac aacgctgaac tctgaaagcg cattaaatgt tgctattaat gaacattttg
      781 gactgaaggt ggcttacaac gtaacatgga actctgaacc tccagagtct gcaccggaac
      841 ataccgatcg tcggacaacc ttatcgctgg gctacagcat gtaaaattat ctgccggacc
      901 gtgttatgcg gtctggctgt cttcgtttgt cactgctcag ccccaaatca ttgctgccca
      961 acgtagcaac aacatagcgc cacagattgc cagtagcacc accaccatct tccagtgttt
     1021 tttatcaaag cgtttagtcg gcataccagt gtcctgcata tctcttgtgc gtgataacgg
     1081 agtgtaacaa aagggacagg catcaaggga ttttttgtta ttacagccgt cgggtggcgt
     1141 tgtacacctg atcactgttt ttgcggttta tgaaaaaaat aaaattatgt atcgatatat
     1201 aaatatctgg ttataaatac gtctttagtt catcagcact aaaactcagg ctttggctga
     1261 ttacgtttat agcagcataa tatgtttcta cagttttata atgattatga tgaatggcta
     1321 tatgaacagc tgacatgttg tcatgatttt tagcggccaa cagtgtaaat aactgacgtt
     1381 ggctaaaaga atattttttt caaaagtacc caacgttgat atataagata acacgacatc
     1441 tttacttact gacaaagtat gattgtctat atataaaatc gcggacatat gactgacatt
     1501 gtttgccatc attcgacaga agaaaatatc taaagcgaag ccaaaatcct tactcgcgat
     1561 aacgcgactc cctgcaggag gatggcgaag taatgatctg aactccgcgt cccggactaa
     1621 gggaacatgc tgctgtatgg attcaacgga ggagtaatgt ttaatcagat tgactttgcc
     1681 ttttccattt ctcagtgaat caagggcgta ttgcaatgac agatggcgac aaaaaatagc
     1741 gtcagaagga gattgcgaaa aaaatgcact accattgaga tttactgttg gttgtatagt
     1801 tttttctcta tggatttttt tagacagttt catacaatgg ttacgatttt cggtagttaa
     1861 ctgttcctgt tcaatataat ccagtatgct tcgcaaatgg ttagctttaa taggcgtggt
     1921 ttccagtaat aaggaaatga tttctttttt atagtgtaat gtaatgagta tattttctgc
     1981 atggatgggt gaagtggcaa agttaatggt aaaaagggca gtggaataac aattttgtga
     2041 tatttttcga cagtttgcgg taaattcctg agtaacacag tatgacagca ttgaacttaa
     2101 gtcagaggta tcaagtaggg tgatgagttg gcacgcaaat tgattaatag gctctgatga
     2161 aggcgttggt atgtcgctat cgatgtgcat tctatgagga gacttataat gagttctgat
     2221 aataatgtcg ttttgactca taatctattc gccttatata tgtctggagg ttttgaggat
     2281 gatgattaat tattatcgcc agaacgaaat gtgcacgaaa aattgctata agaattgtgt
     2341 cacttttatc attgtgatat gaccacttcg tcattaatct tatgatcgtt taaatagtac
     2401 cgccaaatta cgcaacgaac tttgctggat tcagtttatg ctgctgcaaa tccgctcgca
     2461 gtaaaccttt cagacgctcc gtgatgttat cagtggttct ttaagcgttt tgctggtgta
     2521 ctcactacaa acgaattgcg aatcgaatca atgtgaaacg gaaaggtaca atctcccttt
     2581 ttttagtgtt aacatcgttc aaccgggtgg aaggtgagag gttaatgtct ttcatttgtg
     2641 tacctgattg cgaaccaatt tagcatttgt tggctagatg gtttcgcaat gaactgttaa
     2701 taaacaaatt tttctttgta tgtgatcttt cgtgtgggtc accactgcaa ataaggatat
     2761 aaaatgcctg ttataactct tcctgatggc agccaacgcc attacgacca cgctgtaagc
     2821 cccatggatg ttgcgctgga cattggtcca ggtctggcga aagcctgtat cgcagggcgc
     2881 gttaatggcg aactggttga tgcttgcgat ctgattgaaa acgacgcaca actgtcgatc
     2941 attaccgcca aagacgaaga aggtctggag atcattcgtc actcctgtgc gcacctgtta
     3001 gggcacgcga ttaaacaact ttggccgcat accaaaatgg caatcggccc ggttattgac
     3061 aacggttttt attacgacgt tgatcttgac cgcacgttaa cccaggaaga tgtcgaagca
     3121 ctcgagaagc ggatgcatga gcttgctgag aaaaactacg acgtcattaa gaagaaagtc
     3181 agctggcacg aagcgcgtga aactttcgcc aaccgtggtg agagctacaa agtctccatt
     3241 cttgacgaaa acatcgccca tgatgacaag ccaggtctgt acttccatga agaatatgtc
     3301 gatatgtgcc gcggtccgca cgtaccgaac atgcgtttct gccatcattt caaactaatg
     3361 aaaacggcag gggcttactg gcgtggcgac agcaacaaca aaatgttgca acgtatttac
     3421 ggtacggcgt gggcagacaa aaaagcgctt aacgcttacc tgcagcgcct ggaagaagcc
     3481 gcgaaacgcg accaccgtaa aatcggtaaa cagctcgacc tgtaccatat gcaggaagaa
     3541 gcaccgggta tggtattctg gcacaacgat ggctggacca tcttccgtga actggaagtg
     3601 tttgttcgtt ctaaactgaa agagtaccag tatcaggaag ttaaaggtcc gttcatgatg
     3661 gaccgtgtcc tgtgggaaaa aaccggtcac tgggacaact acaaagatgc aatgttcacc
     3721 acgtcttctg agaaccgtga atactgcatt aagccgatga actgcccggg tcacgtacaa
     3781 attttcaacc aggggctgaa gtcgtatcgc gatttgccgc tgcgtatggc cgagtttggt
     3841 agctgccacc gtaacgagcc gtcaggttcg ctgcatggcc tgatgcgtgt acgtggtttt
     3901 acccaggatg acgcgcatat cttctgtact gaagaacaaa ttcgcgatga agttaacgga
     3961 tgtatccgtt tagtctatga tatgtacagc acttttggct tcgagaagat cgtcgtcaaa
     4021 ctctccactc gtcctgaaaa acgtattggc agcgacgaaa tgtgggatcg tgctgaggcg
     4081 gacctggcag ttgcgctgga agaaaacaac atcccgtttg aatatcaact gggtgaaggc
     4141 gctttctacg gtccgaaaat tgaatttacc ctgtatgact gcctcgatcg tgcatggcag
     4201 tgcggtacag tacagctgga cttctccttg ccgtctcgtc tgagcgcctc ctatgtgggc
     4261 gaagacaacg agcgtaaggt accggtaatg attcaccgcg caattcttgg gtcgatggaa
     4321 cgtttcatcg gtatcctgac cgaagaattc gctggtttct tcccgacctg gcttgcgccg
     4381 gttcaggttg ttatcatgaa tattaccgat tcacagtctg attacgttaa cgaattgacg
     4441 caaaaactat caaatgcggg cattcgtgtt aaagcagact tgagaaatga gaagattggc
     4501 tttaaaatcc gcgagcacac tttgcgtcgc gtcccgtata tgctggtctg tggtgataaa
     4561 gaggtggaat caggcaaagt tgccgttcgc acccgccgtg gtaaagacct gggaagcatg
     4621 gacgtaaatg aagtgatcga gaagctgcaa caagagattc gcagccgcag tcttaaacaa
     4681 ttggaggaat aaggtattaa aggcggaaaa cgagttcaaa cggcgcgccc taaccgtatc
     4741 aatggcgaaa ttcgcgccca ggaagttcgc ttaacaggtc tggaaggcga gcagcttggt
     4801 attgtgagtc tgagagaagc tctggagaaa gcagaagaag ccggagtaga cttagtcgag
     4861 atcagcccta acgccgagcc gccggtttgt cgtataatgg attacggcaa attcctctat
     4921 gaaaagagca agtcttctaa ggaacagaag aaaaagcaaa aagttatcca ggttaaggaa
     4981 attaaattcc gtcctggtac agatgaaggc gactatcagg taaaactccg cagcctgatt
     5041 cgctttctcg aagagggtga taaagccaaa atcacgctgc gtttccgcgg tcgtgagatg
     5101 gcgcaccagc aaatcggtat ggaagtgctt aatcgcgtga aagacgattt gcaagaactg
     5161 gcagtggtcg aatccttccc aacgaagatc gaaggccgcc agatgatcat ggtgctcgct
     5221 cctaagaaga aacagtaagg ccttcaagta gcactgtctg tggagccttt gggcttcaca
     5281 ggcattgttc gcctttgttt cgtttattaa caatgcgaag tggaagttat taaaatgcca
     5341 aaaattaaga ccgtacgcgg tgctgctaag cgcttcaaaa aaaccggtaa aggtggtttt
     5401 aagcataagc acgctaacct gcgtcacatt ctgaccaaaa aagcgaccaa acgtaaacgt
     5461 cacctgcgtc cgaaagccat ggtttccaaa ggcgatctgg gcctggtaat cgcgtgcctg
     5521 ccgtacgcat aagccgttaa cgttttttaa ctttttaatt agaatataga tacaggagag
     5581 cacatatggc tcgcgtaaaa cgtggtgtta ttgcacgcgc acgtcacaag aaaattttga
     5641 aacaagctaa aggctactac ggtgcgcgtt ctcgcgtata ccgcgttgcc ttccaggctg
     5701 ttatcaaagc tggtcagtat gcttaccgtg accgtcgtca acgtaagcgt cagttccgtc
     5761 aactgtggat tgcgcgtatc aacgcagcag cacgtcagaa cggtatttct tacagcaaat
     5821 tcatcaatgg cctgaaaaaa gcctctgttg aaatcgaccg taagatcctg gctgatatcg
     5881 cagtattcga caaagtagcg ttcaccgctc tggttgaaaa agcgaaagca gctctggcat
     5941 aagccagttg aaagagggag ctagtctccc tcttttc
//
LOCUS       DAZGQL010000178         5659 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.179, whole genome shotgun sequence.
ACCESSION   DAZGQL010000178 DAZGQL010000000
VERSION     DAZGQL010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5659
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.179"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            1..139
                     /locus_tag="ACHKIR_004881"
                     /pseudo
     CDS             1..139
                     /locus_tag="ACHKIR_004881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
     gene            complement(338..1462)
                     /gene="yhhJ"
                     /locus_tag="ACHKIR_004882"
     CDS             complement(338..1462)
                     /gene="yhhJ"
                     /locus_tag="ACHKIR_004882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO9992507.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKVWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            complement(1462..4197)
                     /gene="rbbA"
                     /locus_tag="ACHKIR_004883"
     CDS             complement(1462..4197)
                     /gene="rbbA"
                     /locus_tag="ACHKIR_004883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HFO9992508.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGSVMVLGGDMRDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYHPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     ACLQEAAGQSNEAEAPPVIHDTTHAARQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMVPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCALSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            complement(4194..5261)
                     /gene="yhiI"
                     /locus_tag="ACHKIR_004884"
     CDS             complement(4194..5261)
                     /gene="yhiI"
                     /locus_tag="ACHKIR_004884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFO9992509.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            complement(5324..5437)
                     /gene="yhiY"
                     /locus_tag="ACHKIR_004885"
     CDS             complement(5324..5437)
                     /gene="yhiY"
                     /locus_tag="ACHKIR_004885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhiY"
                     /protein_id="HFO9992510.1"
                     /translation="MMTTLLPVFTKPSPLALNALCAGRICHFLLIPDGRIR"
BASE COUNT         1305 a         1637 c         1473 g         1244 t
ORIGIN      
        1 aaaatttcac gagtgcttta ttaactggat gcgtgactgc cattcttcag atgataaaga
       61 cgtcattgta attgatggaa aaacgctccg gcactcttat gacaagagtc gccgcagggg
      121 agcgattcat gtcatttctc gcgtcagtcc ttgcggggag cgggctttcg taatcttgcc
      181 ctgaataccg gactgagtat aaaaaagccg gttaactgaa actgtccagg ttttggtggt
      241 cagttcataa acgccttatc cggcctaaaa aaaactaaaa ttcaataaat tgcaccaatg
      301 cgtagaccct ataagcttac gcatcgggca actgtaatta cgccattgtc ccaattgtct
      361 tcctgaatct cagcagtgcg atggtgaaaa acgcgccgcc aattgccatc agtgtcagga
      421 actgcggcca gacgatttcg aatccggcac cacggtagag aatggcctgc gcgaggctaa
      481 caaagtgtgt cgtcggcatg gtcagcataa tgtcctgcac catctgcggc atactttcgc
      541 gcggcgtgga accaccggaa agcatttgca gcggcagcag caccagaatc accagcagcc
      601 ccagttgcgg cattgaacgc gctatcgtcc ccataaaaat accgattgac gtggtggcaa
      661 acagactgag cgccacgccc agcataaaca gcgggatcga gccttctatc ggtacgccca
      721 gcacgccttt taccatcagc accagcgata atcccgacac caccagcacc accagcccca
      781 tcgaccagac ctttgccatc atgatctcaa acggcgttat cggcatcacc agtaagtgtt
      841 ccaccgtgcc gtgttcacgc tcgcggatca gcgccgatcc ggtcaataca atcgccagca
      901 tggtaatgtt gttgatgatc gccatcaccc cgccaaacca cgcgggatca aggttcgggt
      961 taaagcgcat ccgtgtttcc agcgatacca acggttcgct gttatcacgg tagcgcgcga
     1021 caaagctgtt cacttcaccg ttgataatat tctggatata cccattgccg gtaaatgcct
     1081 ggctcatgcg cgtggcatcg acgttcacct gaatatccgg ctggcgtccg gcgaggacat
     1141 cacgctgaaa attaggcgga atatttatcg cgaaggtata acgtccggcg tccagcccgg
     1201 catccatctc atcggcggtg atcatctccg gtggcaaaaa ccacgggcga tagaagctgt
     1261 taacgatccg gttcgataac tgcgattgat ccatatcggc aatggcgatc ggcgcgaggt
     1321 tcaacgatcc tggcgtaacg gtcgctgacg aatacaccga caccgtaaac gagaagacaa
     1381 tcagcgtcag catcgcttta tcaccgagca gactgcgcaa ctctttgata cccagattaa
     1441 aaatattacg taaatggcgc atcatccctc ctgttttttc agcagcagga tgcttaagcc
     1501 catcaccagc ggtatggcta tcagcaacgg gataaaaagt tgccacaaat cagtcagatc
     1561 cagcgctttc gagaacgttc cgcgggcgat agtcagaaaa tgactggtcg ggtaaacctc
     1621 gccgatccaa cgtcctggac cttccagcga agctaccgga tcgatcatcc cggaaaactg
     1681 tgtcgccggg atcaacgtga taattgccgt accaaaaatg gcggcaatct ggcttttcat
     1741 aaaggtggag atcagcagcc ccattccggt ggcgatgatg atatacagca gcgccgccag
     1801 ggtgagagtc aggaaactgc ctttatgcgg tacgccaaat acaaataccg acagggcgca
     1861 gagcaggaaa aagttcagca tccccagcgc gatgtatggc agctgtttgc caagcaaaaa
     1921 ttcgctacgg gtggtggggg tcacgtaaag gttgataatc gaccctagct ctttttcccg
     1981 caccacgcta agggcgctta gcattgacgg gaccatcatc agcagaagcg ggatcaccgc
     2041 cggaacaatc gccggcaggc tttttacgtc cgggttatag cgatagcgcg tctcaatatt
     2101 catcagcccg ctttggctgg cgggtgtgga ctgtcggctc gccacatcct gtagccagct
     2161 ctggtgcatg gcctgcacgt agccttttac cgtttcggcg cggctcggca tcgcaccgtc
     2221 gatccagaca ccgagttcca cgggcgtacc acgcgcgata tcgcgcccga aattaggagg
     2281 gatctcaatt gccaccgtga tatcgcccgc acgcatccga cgatcaagct cgtcataact
     2341 ggtgagcggc ggctgttcga taaagtaacg ggaaccggag agattgagcg tccacgcctg
     2401 gctactgacg gtttgatcgc ggtcaagcac cgcaaagcgc aggttttcca catccatact
     2461 gatgccgtaa cccattatca gcatcaggat caccgttccc atcagcgcca gcgtcgaacg
     2521 taccggatcg cgtcgcagtt ccagcgcttc gcggcggctg tagctaaaca gacggcgcag
     2581 gctaaatccc tgacgcgccg cgtgggtggt gtcgtgtatc acgggcggcg cttcggcttc
     2641 gttgctctgc cctgccgctt cctgcaaaca ggcgataaat gcctcttcca gacttgcggc
     2701 tccgcgtttc tcaaccagtt cctgcggtgt cccgctggcg agcacttttc cggcgtgcat
     2761 cagtgagata cggtcgcaac gttccgcttc gttcataaag tgggtggaga tgaagatagt
     2821 cactttgtcc tggcgcgaga gatcgaccat caactgccag aacatatccc tcgccaccgg
     2881 atcgacgcca gaagtaggct catcgaggat taacatctcc gggcgatgaa tcaccgccac
     2941 cgccagcgaa agccgctggc gaatgccgag cggcaatgac tccggcagaa catcttcaac
     3001 gtcgttgagc ttaaaacgct cgctcatttc agccactcgc gcgggaattt ccgcttccgg
     3061 gatgtgaaac aaacgggcat gtaactcaag gttttgccgc acggtgagtt cgttatagag
     3121 cgaaaacgcc tgcgacatat agcctacccg acggcgggta tcgatatctt ttggatcaac
     3181 cggttgccca aacagccacg cctcaccttc gctggcgggc agcagtccgg tgagcatttt
     3241 catggtggtg gatttaccgc agccgttcga accgaggaat ccaaaaatct ccccgcgtgg
     3301 aatgcggaaa ttgacgtgat caacggcaac gaaggaacca aaacgcatgg tcagatcgcg
     3361 cgcttcgatg gcaatctctg cgttttcagg gtgatacggt gggatcacta ccgcctgatg
     3421 cgcctggcgt tgcgcttgcg gtaacagatt tataaatgct tcttccagcg tggcgctttg
     3481 cgtttgctgc cgcagctctt cggcgctgcc ggttgccagc acttctccgg cattcatcgc
     3541 taccagccag tcgaagcgtt cggcctcttc catgtaggcg gtggcgacca gcacgctcat
     3601 attgctctgc cgctggcgaa tgctgtcgat caaatcccag aactgggcgc gggagagcgg
     3661 gtcaaccccc gttgttggct catcaagaat caacagttcc gggtcgtgga ttaacgcaca
     3721 gcacagccca agtttttgct tcatcccgcc ggagagtttc cctgccggac gatcacgaaa
     3781 cggtgctaac ccggtgctgg tcagcagctc attgattcgc acttcccgct ccgctttgtc
     3841 gtgaccgaac aggcgagcga aaaaatcgac gttttcatac accgacaagg tgtggtagag
     3901 gtttttgccc agcccctgcg gcatccaggc gatgcgcggg caaacgtcgc ggcgatgctt
     3961 cgggtcgcgc atatcgccgc ccagcaccat cacgctgccc tgctcaatga cgcgggcacc
     4021 ggaaatcaac gacaacaagc tcgatttacc gacaccatcc ggtccaatca gcccgaccat
     4081 gcaacgggcc ggaatatcga gtgtgatatt gttcagcgca acggtttttc cataatgctg
     4141 gctcacgccc gccagttgcg cgacaggcgg gacgggaacc agttccagat gcgtcattgc
     4201 ggcaacctca ccaccaggtc gtcaggccac ggaagttctt cattcacccg cacccacgct
     4261 acgcccggca atccggtttt gacatattcc agatgctgct ggagtaattc cggtgggata
     4321 cgcgctttga cacggaacat caatttcagc cgctcatcgc tggtttcgac ggtttttggc
     4381 gtgaactggg cgacgctggc gacaaaactg atggtcgcgg gaatacgcag atctggtgca
     4441 gcatcaagaa tcaaacgtgc ttcaccgccc agtttcagcg tgcccgcctg ttcggttggc
     4501 agaaagaagg tcatatagac gtcgctgaga tcgaccatat tcagcacccg accgcctgcc
     4561 gccagcactt cgcctggctc ggcaacccga tactgcacgc gtccgtcacg cggggctttc
     4621 agttcgctgt catcgatatc tgcggcaatg cgccgttcag tggcttgtgc cgcttcgacg
     4681 cgagtttgcg cctgaatgat attggtgcgt gccgcctcta tagcggcttt agaagcagaa
     4741 acctgagctt ttgccgattc cagcgcagct cgggcgctct cagcggcggc gcggtcgtca
     4801 tccaactgtt gcgcagaaat tgccccccgt tgtgccagtg agcgggaacg cgtatgacgt
     4861 tttgctacgg agtccagttc tgcctggcgt tgattaacca gcgactgtgc ggcacgagtt
     4921 tcgctttggc gttgctccag caaagcctgc gcggcggcaa cggcactttg tgcttctttg
     4981 atttgcgcga tggcttccag tcgctgttcc tgcaacacgc gagtatccat cttcgccagc
     5041 acttcacctt ctcgaacaaa ctggccttct ttcaccagaa tggtgtcgat acgcccggca
     5101 attttgctgg cgatatccac ttccgtcgct tcaatgcgcc cattgctgac agcaaagcct
     5161 tccggcacac ccgccgggcg caacagccac cacgccacga cggcagccac cgccagtgcc
     5221 ccgacaaccc accacgccag atggcgctta ctcttatcca taatcgatcc gccataatcc
     5281 ctgtaagcaa tcgacgcgtg ttacaactgc caccgaacga tgttcagcgg attcttccat
     5341 ccgggataag aaggaaatgg caaatacgac cagcgcacag agcattgagc gccaatgggg
     5401 aaggcttagt gaaaacgggt agtaatgttg tcatcatcag cattcctggc cgtaaatgaa
     5461 acacgcatac tcataagtga aatcaattaa atactaacag ttatattcat gttgtctatt
     5521 tgattgccat caacttgtga tacatcgcga tgttcttccg ttcgctggtg ttttttccaa
     5581 aaatattctg tacaggttca cagattctgt tgcacaggtt tccgtaacag ggaaacctga
     5641 tgactatcac atagcgtta
//
LOCUS       DAZGQL010000179         5655 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.180, whole genome shotgun sequence.
ACCESSION   DAZGQL010000179 DAZGQL010000000
VERSION     DAZGQL010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5655
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.180"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            211..699
                     /locus_tag="ACHKIR_004886"
     CDS             211..699
                     /locus_tag="ACHKIR_004886"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992511.1"
                     /translation="MSTNTPTEINKLFTDPAHIELTKKTLTEITNALDERISEIDKDK
                     HFATYMALQMQSMAMVTAKQTISGLYMKNLRLERELAELRAQMKTDESPTEEINARPE
                     HTNAHITMITRKAERTHICELLRELIETAKETHTCPQETCAAIERLIAMLTNPDELKG
                     RA"
     gene            699..1280
                     /locus_tag="ACHKIR_004887"
     CDS             699..1280
                     /locus_tag="ACHKIR_004887"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFO9992512.1"
                     /translation="MNHLMIDTETLGNGPDAAIFAIGAVFFDPFTGKLGKQFERFIDP
                     VDSERNGGTVNAATAVWWAGQSVDARACLRNADGTELAAVTEFLAFISRNMHDESPGK
                     TLTVWCKGASFDFPILKSAITRTTGDKSIPWCYWNERCMRPLIDMAESTGWRMPGRQG
                     KETIAHTALNDAIYQAKVVSEIWQRFTAPFLSV"
     gene            1318..2292
                     /locus_tag="ACHKIR_004888"
     CDS             1318..2292
                     /locus_tag="ACHKIR_004888"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="HFO9992513.1"
                     /translation="MSKELLSGTPLKWVGGKSRIMNTLKAHLPEADCLVEPFVGGASV
                     FMNTSYSRYILGDSNGALINFWRTARDNTDSLIDEAKDLFREHNNRTDYLAIRNTFNA
                     ENHAFLSTGISDRRELRLAAMFLYLNRHCFNGLYRVNGKGDFNVPFGGYRTPYFPERE
                     IRAFADKANSTRTLLVHGDFRKTLSCASHLFSMGNTLCVYCDPPYLPFNARDNFTAYG
                     KPFTKDEHVRLRAALDRLSQETGGMTSITISNSDTPETRRIYQGYRMNCIQAPRSVGA
                     RTREPAPEVIATLKQCDACGRHGGGYCPDCGPVTGYETYSAIFATANT"
     gene            2328..2564
                     /locus_tag="ACHKIR_004889"
     CDS             2328..2564
                     /locus_tag="ACHKIR_004889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992514.1"
                     /translation="MNSERNKTLLQGLRHIAHAHEETAKLATAVGKPTVVISSAISQG
                     ITIEAIQVIQDQEREIAALKKLLHVKSAGQGVKQ"
     gene            2561..2767
                     /locus_tag="ACHKIR_004890"
     CDS             2561..2767
                     /locus_tag="ACHKIR_004890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992515.1"
                     /translation="MIKESTVIRFTTHGHQYEVDESLIDQGMTRRDSRNPDIYHIRLI
                     NGSHFRATNVEEVRVLTTSCRRAL"
     gene            2793..3173
                     /locus_tag="ACHKIR_004891"
                     /pseudo
     CDS             2793..3173
                     /locus_tag="ACHKIR_004891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372767.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3104..3331
                     /locus_tag="ACHKIR_004892"
     CDS             3104..3331
                     /locus_tag="ACHKIR_004892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="derepression protein"
                     /protein_id="HFO9992516.1"
                     /translation="MANRKQQRARAERLHIRTEINRRLHRAASVTFIMQSNALHRLND
                     GIAAGYYAAVFSYLAEDLAELQDLINQQHHH"
     gene            3400..3816
                     /locus_tag="ACHKIR_004893"
     CDS             3400..3816
                     /locus_tag="ACHKIR_004893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992517.1"
                     /translation="MKSVKGLSDIQDFFNWMWAVDPRLASRLNDWHDYYRRACKQAAR
                     RVTTYGDFSFTVDDRYRVDVKGDGDGFGLFHINGTDSTAISWSSSEMALVLEMTTHSV
                     AVKGIYTAEDFIGEYQRLLVLYRAAFQAATAGECDA"
     gene            3813..>5655
                     /locus_tag="ACHKIR_004894"
     CDS             3813..>5655
                     /locus_tag="ACHKIR_004894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFO9992518.1"
                     /translation="MSDHNAQLAYPWNAPLPVIDPETFGRAQPTALRQAIQSYINEDI
                     RIDARLDEETVDFLTNTDEGKRINSHFHHDEERRIRLEKLARHKHENPPTLVSEAMAE
                     LRALPPFLAGPLIRDLARLSRKQESARREGLKNNSAYAADNFVRHGLRARLKRIARVN
                     ERFATPAFKATAARERLDELLMLPQLSRDEIQRLATLTATAFSAALERTCDEVIEKTG
                     RGDDDLFTWLLTYQPLAHMALKLGITPPCWPSLEIRPDSRTAPDPEQVPGAVMRLSCA
                     TWWNSQLRHLRDIWREELLRAAGLVSRKTSVYISHEALLDWREKRQRTRDFLKAWDIE
                     NEEGERFSLEDIYWAGLSNPRNRRNEMMACVRGMEQVAEARGDVAFFVTVTAPSRFHS
                     VNDDGSLNPKYCGATVRDASDYLVYRFFAAVRRAINKAGLGWYGVRTVEPHHDGTPHW
                     HMLVFTSPENEARITEIMRNAAIREDRAELGDDISPRFKCEKIDPAKGTPASYIATYI
                     GKNLDASAFMGNDPKTGKPYVDKESGKTMAETVENAIGWAALHRIRQFQFFGIPPRQV
                     WRELRRLAGQMARNPTAPQRLDHDDIDAILAAADVGCFATYITRQGGV"
BASE COUNT         1478 a         1633 c         1469 g         1075 t
ORIGIN      
        1 gcagcatgag catatttctt tcatgggtga ttctgattat ttccgtgctt atggcgattg
       61 gcattatgcg aattatcgaa gcgatgaaaa atatcgaatc tttatttact ggcgaagaac
      121 gccgtaaata aataaccaac cggacagaaa acattaaaac catccgcatc tgcggagggc
      181 ttcactcaat ccagaaacgg agaaacagct atgagcacca atacacctac agaaataaac
      241 aaactattta ccgacccagc gcatattgaa ttgacgaaaa aaacgctgac tgaaataacc
      301 aatgcactgg atgaaagaat atcagaaata gataaagaca agcattttgc cacctacatg
      361 gccctgcaaa tgcaaagcat ggcaatggtg acagcaaaac agaccatcag tggactctat
      421 atgaaaaacc tgcgcctcga gcgcgaactg gcagaactcc gggcacaaat gaaaacagac
      481 gaatccccca ccgaagaaat aaacgccaga ccggaacaca cgaacgcgca tatcaccatg
      541 atcacaagaa aagccgagcg tactcatatt tgcgaattac tgcgcgagct gattgagaca
      601 gcgaaagaga cacacacctg cccacaggaa acctgtgcag ccattgaacg actgattgca
      661 atgctcacca acccggacga actgaaggga cgcgcataat gaaccatctc atgattgaca
      721 ccgaaacact gggtaacgga ccggacgcag ccatattcgc gatcggggca gtgtttttcg
      781 acccgttcac cggaaagctg ggaaaacagt ttgagcgatt cattgacccg gtggattcag
      841 agcgcaacgg tggaaccgtc aatgcagcaa ccgctgtatg gtgggccgga caatccgtgg
      901 acgccagagc ctgcctgcgc aacgcagatg gaacagagct ggcagcagtg accgaattcc
      961 tggcgttcat ttcacgcaat atgcatgacg aaagcccggg caagacactg accgtatggt
     1021 gcaagggcgc ttcgtttgac ttcccgatcc tgaaatccgc tatcacgcga acaaccggag
     1081 ataaaagcat cccctggtgt tactggaacg agcgctgtat gcgtccgctt atcgacatgg
     1141 ctgaatcaac aggctggaga atgccaggcc gacaaggaaa agagaccatt gctcataccg
     1201 cactgaacga cgccatttac caggcgaagg ttgtttcaga aatctggcag cgcttcactg
     1261 cgccattcct gagcgtatga agcggacaca cgaaacctgt aacaaagagg ccgcgttatg
     1321 agcaaagaac tgttatcagg cacaccactg aaatgggtgg gcggaaaaag ccgcatcatg
     1381 aacacactga aggcgcacct gccagaagcc gactgtctgg ttgagccgtt cgtgggcggc
     1441 gcatcggtat tcatgaacac cagttacagc cgctacatcc ttggagattc caatggtgcg
     1501 ctgattaatt tctggcggac agcacgggac aacaccgaca gtctgatcga cgaggcaaaa
     1561 gacctgttcc gggaacacaa caacaggact gactatctcg ccatccgaaa cacctttaat
     1621 gcagagaatc atgcattcct gagcacggga ataagcgacc gtcgggaact gcgactggcc
     1681 gccatgtttt tgtatctcaa ccggcactgc tttaatggcc tgtaccgggt gaacggaaaa
     1741 ggtgacttta acgttccttt cggtggctac cgaactccct acttccctga acgggaaatc
     1801 cgcgcatttg cagacaaggc aaacagcacc cgaacactgc ttgttcatgg cgattttcga
     1861 aaaacactga gctgtgccag ccacctgttc agcatgggta acacgctttg cgtgtactgc
     1921 gatccccctt acctgccttt caatgcccgg gacaatttca ccgcctacgg aaaacccttc
     1981 acgaaggacg aacacgttcg cctgagagca gcgcttgacc gtctttcaca ggaaacgggc
     2041 ggcatgacca gcatcaccat atccaattcc gacacaccgg aaaccagacg tatttatcag
     2101 ggttaccgga tgaactgcat acaggcacca cgaagcgttg gcgcaagaac ccgcgaacca
     2161 gcaccggagg ttatcgccac cctgaaacag tgcgacgcat gcgggcgcca tggcggcggc
     2221 tactgcccgg actgcgggcc ggtgacggga tatgaaacgt acagcgcaat atttgccact
     2281 gccaatacgt gaataaacag atgaatcctt aacaccggag tcaaccaatg aatagcgaaa
     2341 gaaacaaaac tcttttacag gggttacggc acatcgccca tgcacacgag gaaacagcca
     2401 aacttgcaac tgccgttgga aaaccaacgg tcgttatctc ttcagcgatt tcacagggga
     2461 tcaccataga agcaattcag gtaattcagg atcaggaaag agaaatcgcc gcccttaaaa
     2521 agctactcca cgttaaatct gccgggcaag gagtcaagca atgatcaagg agtcaaccgt
     2581 tatcagattc acaacccacg gccatcagta cgaagtcgac gaaagcctga ttgatcaggg
     2641 catgacacgc cgggattcac gcaatcccga tatatatcac atccgcctta tcaacggttc
     2701 gcacttccgc gccacgaacg tggaagaagt acgcgtgctt accacaagct gccgtcgtgc
     2761 tctgtgaact gttaccggac tgagtgatca tcatgcacac acaaaaaaac cgcttgccag
     2821 cgttcgcctg gtcgggttac agttttcccg cacctcataa aacgggtgcc gggatttcca
     2881 ccccgccgat aaccaaagcg cacaaccgcg ccagcggttt ttttgtgcgt actgtattgc
     2941 cacgtctttt tcgcgtcaga attatggcgg ggcgtacggg gccgacttcg gtcgggccgg
     3001 gttctttggt taccggttgt ggaaaccctg tacgcctcgc caccccgagt tttccacctc
     3061 tggatggtga gttttcaaaa ttcacaacca aagaggccac accatggcaa accgcaaaca
     3121 acagcgcgcc cgcgctgagc gtcttcacat ccgcactgaa attaaccgca gactccatcg
     3181 ggctgcatct gtcaccttca tcatgcagtc aaacgcatta catcgactta acgatggtat
     3241 cgctgccggg tattacgcgg cagtcttcag ctatctggcc gaagacctgg cagaactgca
     3301 ggaccttatc aaccagcaac accaccacta aacaacatcc tgaagccatc ccgcacccgt
     3361 gcgcgggagg cttccgcacg tcggttaccg gaggttctca tgaagagcgt taagggactt
     3421 tcagatattc aggatttttt taactggatg tgggcagtgg atccccggct ggcttcccgc
     3481 ctgaacgact ggcacgacta ttaccgccgc gcctgtaaac aggccgccag acgcgtcacc
     3541 acatacgggg atttctcctt caccgttgac gaccgttacc gcgtggacgt gaaaggcgat
     3601 ggcgatggct ttggtttatt tcacatcaac ggcaccgaca gcaccgccat cagctggtcg
     3661 tcgtcagaga tggcgcttgt gctggaaatg acaacgcaca gcgtggccgt gaaaggcata
     3721 tacaccgcag aagactttat cggtgaatac cagcgccttc tcgtgctgta ccgcgcggcg
     3781 tttcaggcgg cgactgccgg ggagtgcgac gcatgagtga ccacaatgca cagctggcgt
     3841 acccgtggaa cgccccgcta ccggttatcg atcctgaaac gttcggcaga gcgcaaccaa
     3901 ccgcattacg ccaggccatt cagagctaca tcaacgaaga cattcgcatt gatgcaagac
     3961 tggatgaaga gaccgtcgac ttcctgacaa acacagacga aggcaagcga attaattcgc
     4021 attttcacca cgacgaagag cgtcggatca ggctggaaaa actggcgcgg cacaaacacg
     4081 agaatccgcc aacactggta tctgaagcca tggcagagct gcgcgccctt ccgccgtttc
     4141 ttgccggtcc gctcatccgt gacctggcac gcctgagccg caaacaggag agcgcacgcc
     4201 gggaagggct taagaacaac agcgcatacg cggcggataa cttcgttcgt cacgggctgc
     4261 gtgcccgcct gaaacgtatc gcccgtgtga atgaacgctt tgccaccccg gcctttaagg
     4321 ccaccgccgc gcgcgaacgg ctggatgaac tgcttatgct gccgcagctg agccgtgacg
     4381 aaatccagcg ccttgccacc ctgaccgcca ccgcgttttc cgccgcactg gagcgcacat
     4441 gtgatgaggt catcgaaaag accggcaggg gtgacgatga cctgttcacc tggttactga
     4501 cctaccagcc actggcacac atggcgttaa aactgggtat cacgccaccc tgctggccgt
     4561 cgctggaaat caggcccgac agccgcaccg cccccgaccc ggaacaggtg ccgggtgcgg
     4621 tcatgcgcct ttcctgtgcg acatggtgga acagtcagtt aagacacctc cgggacatct
     4681 ggcgtgaaga gctgttacgc gccgcaggcc tggtgtcacg taagacgtcg gtatatatca
     4741 gccatgaagc gttactggac tggcgcgaaa aacgccagcg cacccgcgat tttctgaagg
     4801 cctgggacat cgagaacgaa gaaggagaac ggttcagcct tgaggatatc tactgggcag
     4861 gtctctctaa tccgcgtaac cgccgtaacg agatgatggc ctgcgtccgg ggaatggagc
     4921 aggtcgccga agcacgcggc gacgtggcgt ttttcgtcac cgtcacagcc ccttcccgtt
     4981 ttcacagcgt gaacgatgac ggcagtctga acccgaaata ctgcggggca acggtacgtg
     5041 atgccagtga ttatctggtt taccgctttt tcgccgccgt ccgcagggcc atcaacaagg
     5101 ccgggctggg ctggtacggc gtgcgcaccg tggagcccca ccacgacggt acaccgcact
     5161 ggcacatgct ggtattcacc agcccggaaa acgaagcccg tatcactgaa atcatgcgca
     5221 acgccgccat cagggaggac cgcgcagagc tgggtgatga tatctccccg cgttttaaat
     5281 gcgaaaaaat cgacccggca aaaggcacac cggcaagcta tatcgcgacc tatatcggta
     5341 agaacctgga cgccagcgcc ttcatgggga atgacccgaa aaccggcaaa ccttacgtcg
     5401 ataaggaatc aggcaaaacc atggccgaaa ccgtggagaa cgccatcggc tgggcggccc
     5461 ttcaccgcat ccgccagttc cagttcttcg gtatcccgcc ccgccaggta tggcgcgaac
     5521 tccgccgcct tgccgggcag atggcccgta acccgactgc cccgcagcgt ctggaccatg
     5581 acgacattga cgccatactg gccgccgccg acgtgggatg ttttgccacc tatatcaccc
     5641 gccagggtgg tgtgc
//
LOCUS       DAZGQL010000180         5649 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.181, whole genome shotgun sequence.
ACCESSION   DAZGQL010000180 DAZGQL010000000
VERSION     DAZGQL010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5649
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.181"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            697..867
                     /locus_tag="ACHKIR_004895"
                     /pseudo
     CDS             697..867
                     /locus_tag="ACHKIR_004895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526115.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1083..1916)
                     /locus_tag="ACHKIR_004896"
     CDS             complement(1083..1916)
                     /locus_tag="ACHKIR_004896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFO9992519.1"
                     /translation="MMKLGFNEATCLRNSSLEKDLVLAERFGYDYIEIRLDMLQTWLQ
                     EHTLVELADIFSVSHLKPWGYNSLEDITFCDAGRWSEIRQQLVLACYAGEVVGGDCLV
                     VVPTIREGAHFSDRDTVRDSVKRLREMSALAAESQMRLAFEPIGSAGCCVRSLAMAME
                     IVDAVDRSNVGLAIDAFNLYLYDQWQDLSTLRQVPVEKIFVYHIDDADRLPVAVLDHC
                     HRLFPGNGAIPLYMITHELVKKGYTGICSLELFNPGYWQMAASEVFAIGAEKTRPFLA
                     D"
     gene            complement(1923..3260)
                     /locus_tag="ACHKIR_004897"
     CDS             complement(1923..3260)
                     /locus_tag="ACHKIR_004897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 30 beta sandwich
                     domain-containing protein"
                     /protein_id="HFO9992520.1"
                     /translation="MKGRLITSDPWNQQFMVELEVFFSPRQPACSELISVFPNHSLQQ
                     IDGFGGSFTESAGVVFNSMSDKTKAQFLNLYFSPQEHNYSLGRMPIQSCDFSLSNYAY
                     VDSSEDLRQGRISFSRDESHLMPLISAALRINPQIKLMASPWSPPAFMKTNYDMNGGG
                     KLRYEYYADWADIIIDYLLEYRRHGISVKALSVQNEPVAIKSWDSCLYSVEEETTFAV
                     HYLRPRLARYGMNEIEIYIWDHDKDGLIDWAERAFADEKDGDSINGMAFHWYTGDHFS
                     QIQYLARKLPNKKLLFSEGCVPMENDEGGQIRHWHTYIHDMIGNFKSGCSGFIDWNLL
                     LDSDGGPNHQSNLCEAPVQYDALNDILWCNHSWYGIGHFCRFVHPGAKVMLTSSYDTQ
                     LEDVGFVNPDGERVLVVYNRDTRERHFRVVDGDRELALTLPPSGASTLVWRQE"
     gene            complement(3358..4368)
                     /gene="iolG1"
                     /locus_tag="ACHKIR_004898"
     CDS             complement(3358..4368)
                     /gene="iolG1"
                     /locus_tag="ACHKIR_004898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase IolG1"
                     /protein_id="HFO9992521.1"
                     /translation="MTLKAGIVGIGKIGADHLRRLANTISGVEVVAVCDIVEGRAQAA
                     LDKYEIKAKDYKDYHDLINDEDVEVVVITASNEVHADVAVAALNANKYVFCEKPLAVT
                     ADDCQRVIGAERKNGKRMVQVGFMRRYDKGYVQLKNIIDSGEIGQPLMVHGRHYNVST
                     VSDYKTPQAIYETLIHEIDVMHWLLNEDYRTVKVYFPRQSSLVTTLRDPQVVVMETTS
                     GINIVVEVFVNCQYGYDIHCDVTCEKGMVELPTVATAAVRKEAKYSTDILVDWKQRFI
                     DAYDIEFQDFFDRLNAGLPLAGPTSWDGYLAAVTADACVKSQETGNTEFVELPAKPDF
                     YK"
     gene            complement(4386..5291)
                     /gene="iolE"
                     /locus_tag="ACHKIR_004899"
     CDS             complement(4386..5291)
                     /gene="iolE"
                     /locus_tag="ACHKIR_004899"
                     /EC_number="4.2.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050114 - myo-inosose-2 dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inosose-2 dehydratase"
                     /protein_id="HFO9992522.1"
                     /translation="MYSVKESIKWGIAPIGWRNDDLPEIGKDNTYKQILSDAALTGFS
                     GTEVGGCYPLDPTELNIELALRHLEIPGKWFSSFIIRDGIESVKKAFEQHCAYLQAVH
                     AYIAVVSEQTYSIQGIIDKCVYTEKPYFNDIEWQQLCSGLNELGMIANTYGLKLAFHH
                     HMGTGVQTLQEVDRLMENTDPQLVNLLFDTGHIYVSDGDVMSLLCKHFSRIKHVHFKD
                     VRDEKLKACRAGKKSFLNSFLDGMFTVPGDGNIDFKSVLAYLVENHYSGWIVVEAEQD
                     PNIYNPLEYAQKGKKHIDGLLGNYI"
BASE COUNT         1646 a         1385 c         1065 g         1553 t
ORIGIN      
        1 ttaactggtt gctgatataa gtttttcacc acgcataaac actgcccatg cgatcctggc
       61 aatcttattt gccagctcta tgatgacgat attcgttggt cgacgtgcct gctgggctta
      121 aatctattcc cccggggatc cggctttatt gcaaccataa agctgcgagc ccctatgtaa
      181 ttcgctgact cacacctcat ttactgcgcg ggattcaagc ccaaaacccc atttaggttt
      241 agtgagtccc tgtcttacct gtctttgtca tcacgttgac caccagagca acctttctgc
      301 attcacccat ttaagtaacc aaaatgagta tcaggcctga tatatacaga actgaattcg
      361 ttaattaatt attaatcagt taaattttcc gttactggga tgaggtgtac acttggcctc
      421 tcccaaaatc cgcggaaccg cccctggccc ctattcagca tttcaggctc taagttgagg
      481 actgaccgga ttgacatagg gcctgctgac gccattcatt gactaccaac agatctttct
      541 tgttatttat agaaaaccac cagccagcct agtggttcag taaaacttcc aactttgagg
      601 ctgttgtcaa aacccctttt tgatttgtta aaacaccttg cagtctacca actgtaagcg
      661 tcaaacaaga aatcaaaagg ggattccaat gggtgaatga aaaagggctt aacgaacacc
      721 cgatggaact gtaaatatca catagttttt gcgccaaaat accgaagaca gatgttctac
      781 agagaaaagc gaagtgcaag agcaccaggc aatattttga gaaaactgtg tgagtggaaa
      841 agtgtacgga ttctggaagc tgaatgctga gcggatcata tccatatgct tgtgaattta
      901 cggggcgtaa gtaaggaagt tgaatgaaaa tgtcagatca tatgcgcctg tcagccaggg
      961 cgaggcagaa aacgaatctt taagacacat ctgaaaaacc tccggctatg ccggaggaga
     1021 tttattctga tcacctgctt attaccgggc acagcgacag ttcatttgca gttacgtcag
     1081 cgttaatccg ctaagaaagg tcgcgttttt tccgctccga tagcaaagac ctcgctggct
     1141 gccatttgcc agtaacctgg gttaaacaac tccagtgaac agatccctgt ataacctttt
     1201 ttcaccagtt catgggtgat catgtacagt ggtatcgcac cgttgcctgg aaacagacga
     1261 tgacaatgat ccaacacagc tacaggtaaa cggtcagcat catcaatatg atagacaaag
     1321 atcttttcca ccggaacctg tcgcagcgta ctcagatcct gccattgatc gtacagataa
     1381 aggttaaagg catctattgc cagaccaacg ttactccgat caactgcgtc cacgatctcc
     1441 atcgccatcg ccagactgcg tacgcaacat cccgcagagc cgataggctc aaaggccagt
     1501 cgcatctggc tctctgccgc taatgctgac atctcacgca aacgtttcac tgaatctctt
     1561 accgtgtcac gatctgaaaa atgagcacct tcacgaatgg tcggcactac aaccaggcaa
     1621 tcaccaccta cgacttcccc tgcataacag gctaaaacca gttgttgccg tatttccgac
     1681 cagcgcccgg catcacaaaa tgtgatgtct tccagcgagt tgtagcccca tggcttaaga
     1741 tggcttacag aaaaaatatc ggccagttca accagagtgt gttcctgcag ccacgtctgc
     1801 aacatatcca gacggatctc aatatagtca tatccaaaac gttccgctag gaccagatcc
     1861 ttctccaggc tggaattacg cagacaggtc gcttcattga atcccagttt catcattcag
     1921 acttactcct gccgccatac caatgtactg gctccagaag gcggcagcgt taatgcgagt
     1981 tctctgtcgc catcgaccac ccggaaatgc ctttctcgcg tatcgcgatt atataccact
     2041 aaaactcgct caccatcagg attaacaaaa cccacatctt ccagttgcgt atcataactg
     2101 gaagtaagca taactttcgc accgggatgc acaaagcggc aaaagtgtcc aattccgtac
     2161 caagagtggt tacaccagag aatgtcattc aatgcatcgt actgcacagg agcttcgcac
     2221 aaattactct ggtgatttgg gcctccatca ctgtctaaca gcaggttcca gtcgataaat
     2281 ccgctacagc cggatttaaa attaccaatc atgtcatgaa tataggtatg ccagtgacgg
     2341 atctggccac cttcatcatt ctccattggt acacatcctt cggaaaacag cagcttttta
     2401 ttcggcaact tccgggccag atactgaatt tgcgaaaaat ggtcaccggt ataccagtgg
     2461 aaagccatcc cgttgatgct atcgccatcc ttttcgtcag caaaggcacg ttcagcccag
     2521 tcaatcagac catctttatc atgatcccag atatagatct ctatttcatt cattccatag
     2581 cgggcgagtc gtgggcgcag atagtgaaca gcaaaagtag tctcttcttc tacactatac
     2641 agacaggaat cccagctttt tatcgcaact ggttcattct gcactgatag tgctttaaca
     2701 ctgataccat ggcgtctgta ttcgagcaga tagtcgataa taatatctgc ccagtcagca
     2761 tagtattcat accgcaattt cccacctccg ttcatatcat aattagtctt catgaatgcc
     2821 ggtggactcc acggggaagc cattaatttt atttgtggat tgatccgcaa tgccgcggaa
     2881 attagcggca ttaaatgaga ttcatcacgg gaaaaggaaa tccttccctg acgcaggtct
     2941 tcactggaat caacataggc ataattgctc agagagaaat cacagctttg gatcggcatc
     3001 cgccccaaac tatagttatg ttcctgaggc gaaaaataga gattcaaaaa ttgcgccttt
     3061 gtcttatcac tcatgctgtt gaaaacaaca ccagcacttt cagtgaagct gccgccaaat
     3121 ccatcaatct gttgtaacga gtggtttgga aagacgctga ttaattcact acacgctggt
     3181 tgacgaggcg aaaaaaaaac ctccagctca accataaatt gctgattcca gggatcggaa
     3241 gtgattagtc tgcctttcat agagggattc cagataatat aggatgataa attgtgatat
     3301 accggacagg agcatacacc tagctatccg gtatgaaatt atcacatcgc agaaagacta
     3361 tttgtagaag tcaggttttg ctggtaattc cacaaactcg gtgtttcctg tttcctggga
     3421 cttcacacag gcatcagccg ttaccgcagc cagataacca tcccatgatg ttgggcctgc
     3481 cagaggtaat cccgcattca gacggtcaaa gaagtcctgg aactcaatat cataggcatc
     3541 aataaagcgt tgtttccagt ccaccaaaat gtcagtactg tattttgcct ctttacgcac
     3601 cgcagcagta gccacggtag gtaattccac catccctttc tcacaggtca catcacaatg
     3661 aatatcatag ccatactggc aattcacaaa cacctcaacc actatattaa taccggaggt
     3721 cgtttccatc accaccacct gcggatctcg tagagtagtc accaagctgg actgacgtgg
     3781 gaagtagacc ttaactgttc tataatcttc gttgagtaac cagtgcataa catcgatttc
     3841 atgaatcagc gtttcataaa ttgcttgtgg tgttttgtaa tccgataccg tgcttacatt
     3901 ataatgacga ccatgaacca ttaatggttg accaatttct ccgctatcga tgatgttttt
     3961 cagctgtacg tagcctttat cgtagcgacg cataaaccca acttgcacca ttcgttttcc
     4021 atttttacgt tcagctccga tcacacgctg acaatcatct gctgtaaccg caagcggctt
     4081 ttcacagaaa acatatttat tggcatttaa cgctgcaacg gctacatcgg catgtacttc
     4141 attcgaggca gtaattacca ctacttcgac atcttcatca ttaatcagat cgtgatagtc
     4201 tttatagtct tttgctttga tctcatactt atccaaagct gcttgcgctc tgccttcgac
     4261 aatatcgcac acagcaacaa cttctactcc agaaatagtg ttagccagac gtcttaagtg
     4321 atcggcacca attttcccga tccccacaat acctgctttt aaagtcatct tattttcctt
     4381 ttctatcaga tataattacc caataaacca tcaatatgct ttttaccttt ttgtgcatat
     4441 tccaaaggat tatatatatt cgggtcctgc tcagcttcaa cgacaatcca gccggaataa
     4501 tgattctcaa ccaaataagc caatacagat ttaaaatcaa tattcccatc accaggaaca
     4561 gtaaacattc catcaagaaa agaattgaga aatgattttt ttccagcccg acatgctttt
     4621 agtttttcat cccgaacatc tttaaagtga acatgtttaa tacgggaaaa atgcttgcac
     4681 aacagtgaca tcacatctcc atcagaaaca taaatatgac cagtatcaaa cagcaaattc
     4741 actaattgtg gatctgtgtt ttccataagt cgatccactt cctgtaaagt ttgtacgcca
     4801 gtgcccatat gatggtggaa agcaagttta agcccatatg tattggcgat catgcctaac
     4861 tcgttcaatc ctgagcataa ttgttgccat tcgatatcat tgaagtaagg tttttccgta
     4921 tagacacatt tatcaataat tccttgaata ctatacgttt gctcagacac aacagcgata
     4981 taagcatgga cagcctgcaa ataagcgcaa tgttgttcaa aagctttctt cactgattca
     5041 ataccatcac gaataataaa agatgagaac cactttccgg gaatttccag atggcgaagt
     5101 gctaactcta tatttaattc agtcgggtcc agaggataac atccccccac ttctgttcca
     5161 gaaaaaccag taagtgctgc atcacttaat atctgcttat aggtattgtc tttaccaatt
     5221 tcaggcaaat catcatttcg ccatccgatt ggcgcaatcc cccatttaat actctctttt
     5281 acactataca tattccagac tcctgcttaa taaaactttc tcacatcctg ttgcataagt
     5341 aataatcttt ggctgacaat atgctacttt ccacgtggct aaaaaacttt atctatcatg
     5401 agtacagaag tgggaaaaat gtgtcaggaa tcacaaaaaa aacacaaccc tttaatgatt
     5461 ttgtctatgt tttagtcatg ctgaaaaatc acacaatgac cgtaaaataa aaccgtcact
     5521 ttcccactaa ctctggcgtg gacagaatca tcccgtatga ttcccaagtt tggtaaatat
     5581 tattaaatca ggatgtgcaa gctatgattc agtcatacta ggaaaaaagt ttttttgcag
     5641 attcggttg
//
LOCUS       DAZGQL010000181         5626 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.182, whole genome shotgun sequence.
ACCESSION   DAZGQL010000181 DAZGQL010000000
VERSION     DAZGQL010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5626
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.182"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(81..377)
                     /gene="yciI"
                     /locus_tag="ACHKIR_004900"
     CDS             complement(81..377)
                     /gene="yciI"
                     /locus_tag="ACHKIR_004900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HFO9992523.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            601..1320
                     /gene="tonB"
                     /locus_tag="ACHKIR_004901"
     CDS             601..1320
                     /gene="tonB"
                     /locus_tag="ACHKIR_004901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HFO9992524.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            complement(1360..1758)
                     /gene="yciA"
                     /locus_tag="ACHKIR_004902"
     CDS             complement(1360..1758)
                     /gene="yciA"
                     /locus_tag="ACHKIR_004902"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HFO9992525.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(1863..2402)
                     /gene="yciB"
                     /locus_tag="ACHKIR_004903"
     CDS             complement(1863..2402)
                     /gene="yciB"
                     /locus_tag="ACHKIR_004903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HFO9992526.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            complement(2432..3175)
                     /gene="yciC"
                     /locus_tag="ACHKIR_004904"
     CDS             complement(2432..3175)
                     /gene="yciC"
                     /locus_tag="ACHKIR_004904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciC family protein"
                     /protein_id="HFO9992527.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGVFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAVLLIYLFRLYMLIRQ"
     gene            complement(3203..3292)
                     /locus_tag="ACHKIR_004905"
                     /pseudo
     CDS             complement(3203..3292)
                     /locus_tag="ACHKIR_004905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            3532..4170
                     /gene="ompW"
                     /locus_tag="ACHKIR_004906"
     CDS             3532..4170
                     /gene="ompW"
                     /locus_tag="ACHKIR_004906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HFO9992528.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
     gene            complement(4229..4735)
                     /gene="yciE"
                     /locus_tag="ACHKIR_004907"
     CDS             complement(4229..4735)
                     /gene="yciE"
                     /locus_tag="ACHKIR_004907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFO9992529.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSISGYV
                     FEQFEIACYTSLLAAAKNAGDTASIPIIEAILNDEKHMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(4781..5281)
                     /gene="yciF"
                     /locus_tag="ACHKIR_004908"
     CDS             complement(4781..5281)
                     /gene="yciF"
                     /locus_tag="ACHKIR_004908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFO9992530.1"
                     /translation="MNMKTIEDVFIHLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETYGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            complement(5367..5546)
                     /gene="yciG"
                     /locus_tag="ACHKIR_004909"
     CDS             complement(5367..5546)
                     /gene="yciG"
                     /locus_tag="ACHKIR_004909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFO9992531.1"
                     /translation="MAEHRGGSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
BASE COUNT         1530 a         1320 c         1299 g         1477 t
ORIGIN      
        1 aatgcaatga ctgacctata aggaaaggcc aaacaagaac acggttgcta aaaccgtgcc
       61 cttaaatatt gaatttctat tcagaacact ttcttaaatg gtttcactga aacgtgttca
      121 tagactcctg ccgctacgta cgggtcagca tcggcccagg cctgagctgc ttccagcgat
      181 tcaaattcag caataacggt tgagccagta aatcccgcag cccctggatc gttactgtct
      241 actgctggca ttggaccggc ggtcaacaat cgaccttcat catgcagtaa ctgtaaacgt
      301 gctaaatgtg ccggacgaac ggaaaggcgt ttttcgaggc tatcagcttt gtcttgagcg
      361 taaataacat acaacacggg cacaactcct tgttcgggaa agttgtaaag tacgttattt
      421 gaaagggcga agatctgcaa cggaaagatg atgtctttgt taagaccatt taaaaagtaa
      481 ggtcaattac gccaaaaatg acattttcac tgatcctgat cgtcttgcct tattgaatat
      541 gattgctatt tgcatttaaa atcgaggcct ggtttttcta ctgaaatgat tatgacttca
      601 atgacccttg atttacctcg ccgcttcccc tggccgacgt tactttcggt ctgcattcat
      661 ggtgctgttg tggcgggtct gctctatacc tcggtacatc aggttattga actacctgcg
      721 cctgcgcagc caatttctgt cacgatggtt gcgcctgcgg atctcgaacc gccacaagcc
      781 gttcagccgc caccggagcc ggtggtagag ccagaaccgg aacctgagcc gatccccgaa
      841 ccgccaaaag aagcaccggt ggtcattgaa aagccgaagc cgaaacctaa gccaaaaccg
      901 aagccggtga aaaaggtaca ggagcagcca aaacgcgatg tcaaacccgt agagtcgcgt
      961 ccggcatcac cgtttgaaaa tactgcaccc gcgcgcccta catcaagtac ggcaacggct
     1021 gcaaccagca agccggttac cagtgtggct tcaggaccac gcgcattaag ccgcaatcag
     1081 ccgcagtatc cggcacgagc acaggcattg cgcattgaag ggcaggttaa agttaaattt
     1141 gatgtcacgc cggatggtcg cgtggataac gtacaaatcc tctcagccaa gcctgcgaac
     1201 atgtttgagc gtgaggtgaa aaatgcgatg cgcagatggc gttatgagcc gggtaagcca
     1261 ggcagtggga ttgtggtgaa catcctgttt aaaattaacg gcaccaccga aattcagtaa
     1321 gcagaaagtc aaaagcctcc gaccggaggc ttttgattat tactcaacag gtaaggcgcg
     1381 aggttttcct tcaggatcaa ccgcgacata cttaaataat gcttctgtcg ctttatagcg
     1441 ttgcccgatg ggttcagacg cgactttttt cacccacact tcaatattaa tgctaaccga
     1501 tgtcgtccct ttctggacac agcgtgcata gcagcacacc acatcgccga ccgcaaccgg
     1561 tcgtaagaaa gtcattcctt caacccgcac ggtcactacg cgaccgtggg caatttcttt
     1621 cgccagaata gcgccgccaa tatccatttg tgacattaac caaccaccaa agatgtcacc
     1681 attggcattg gtatcggcgg gcatggctaa agtacgtaaa acaagatcgc cctgagggac
     1741 gttatgtgtt gtagacatgg taaaaccgac ttaaaaggaa aatcacaggc gcgatgctac
     1801 tatgattttc ctgaggagaa cagaggagat acagcgccag ccccgaagga ctggcagtct
     1861 ggttaggatt tatcttcctg cggcatgtgg cggtagatat agataccgct taacaatgtg
     1921 aagattaagg taagggcggt caggccaaag actttaaagt tgacccaaat attttgcggc
     1981 agccagaatg cgatgtagat gtttgccaga ccgcaaagga taaagaaaac agcccaggcc
     2041 agattcagct tcgaccatac cggttgcggc agcgtgagtt ctttacccag catccgctga
     2101 attagcggct ttttcatcac ccattggctg actaacaggg cacccgcaaa cagggcataa
     2161 atgacagtaa ccttccattt aataaactca tcattgtgga agaacagggt caagccaccg
     2221 aagacgacca ccagaacaaa agtgatcagg gccatcttct caaccttacg aaagcgaacc
     2281 cagctatata taagcacaat cgccgtggcg acgatcagcg ccgcagtagc cgcatagatg
     2341 tcataaatct tgtaaaacgc gaaaaagaca accagcggta aaaaatcaag aaactgcttc
     2401 attttacgat tccgtaatca aatgcttaag gttattggcg aatcaacata tacaggcgga
     2461 acagatagat gagcagtacg gctgaaatca ggttgctcaa ggtgttcgcc agtacggcac
     2521 caatttccgg ggttaatgcg gcaaaagaag aggcaaaaag cagcaacagt gtctttgcca
     2581 gcaaccagct cagtactgcg ggtgccacca gacgcatatt cgcccaagtc agccgcatac
     2641 tgctacgcat cgaggcaaaa acgcccattt tgtcctgtac caacatcacc ggtgccaggg
     2701 ccagtaaaat ggccatgata attcccggaa cgaccaccag catgatgcca atctgtacta
     2761 aaagggtagt cagaaaaatc agaataaata actttggcaa tatcggcgca ctggcaccaa
     2821 tagctcgcag cgcactgact ctctgacccg cagacaccag ctggataatt aatattacgc
     2881 ctccggcgag aatggcgtta ccgattaatc ctgaaaaagt ggacgccgct gaagcctgca
     2941 gcaaaatttg ttgctgttcc ggtgacatat tctgaaccag gtcgaacaac ccactactgc
     3001 cgctaacggg cacgccgtca ttgagctgcg caagctgtgc atcactgggt gagaaaacat
     3061 gccctaacac cactgtgata aacgcacata gcaacgatac caacagaatg gtcataaatt
     3121 gattacggaa gaaatttccg gtgtcacggt atacggactg cgccgtgata gacatgcact
     3181 ctccttgagt tttatgcagg tgttaattag cgggtaattg taccctggtt aagcctcggg
     3241 tggcagcatc aggtcttgta tggaaaagca tatctttgta aagcggaggt aattgctggc
     3301 cttcagacgt gggcaaatcg cggcaagtgg cgatcgagtt aatatttgcg tagcgaaaat
     3361 atttaaaaat tgatttaaat cacattaacc aggattctca atgcaacttc taaattaatc
     3421 cagatcaata aatggtgaat tatcatatgt aatgtgatct atgtaggatc atttgttact
     3481 ccaatgtagg tatattcgtc acgtttttat aaccataacg acggagcgga tatgaaaaag
     3541 ttaacagtgg cggctttggc agtaacaact cttctctctg gcagtgcctt tgcgcatgaa
     3601 gcaggcgaat tttttatgcg tgcaggttct gcaaccgtac gtccaacaga aggtgctggt
     3661 ggtacgttag gaagtctggg tggattcagc gtgaccaata atacgcaact gggcctgacg
     3721 tttacttata tggcgaccga taacattggt gtggaattgc tggcagcgac gccgttccgc
     3781 cataaaatcg gcacccgggc gaccggcgat attgcaaccg ttcatcatct gccgccaaca
     3841 ctgatggcgc agtggtattt tggtgatgcc agcagcaaat tccgtcctta cgttggggca
     3901 ggtattaact acaccacctt ctttgataat ggatttaacg atcatggcaa agaggcgggg
     3961 ctttccgatc tcagtctgaa agattcctgg ggagctgccg ggcaggtggg ggttgattat
     4021 ctgattaacc gtgactggtt ggttaacatg tcagtctggt acatggatat tgataccact
     4081 gccaattata aactgggcgg tgcacagcaa cacgatagcg tacgcctcga tccgtgggtg
     4141 tttatgttct cagcaggata tcgtttttaa ttccgcacaa aaatgacccc gtaattacgg
     4201 ggtcaataag gacatggtat aaagggtatt atttcttcgc ttctacgcca tcagtttcag
     4261 agcgaattaa gaatttctca gttgtttgcg gaatatgctg aattagccag tcggccatgt
     4321 gcttttcatc atttaaaatc gcttcgataa tcggaattga ggctgtatca ccggcatttt
     4381 ttgctgctgc taagagtgag gtataacagg cgatttcaaa ttgctcgaag acatatccgc
     4441 taatagagcc tttgactatt tcatcagaag ggaatatacc accgattgac tgcccaagtg
     4501 cagccatttt actcatggaa tctttaatga ctgaacgtga aatgtcatta cgatcaagaa
     4561 tagtttccag ttgaacaatc tggtttttgg tttcactaag atgttgttca atacgagcgc
     4621 gtagttcagg ataattatct atacgactgg ccatggattc aagcattgat tcggcttgct
     4681 tttccattgc gtgggcgtca cgtaaccagt catgataatg ttcaatacga ttcatgaaaa
     4741 tgtcctcttt ataaaatgac taaaagttaa agttcatatt tcaggcttta ttttcggctt
     4801 tcttatttac gttattaagg gccagatcag tcaatttgat gtcggtggcc ttttcttctt
     4861 ccagggtctc tttcagaagc ttcgctgctt tacgataacc taattgttca gccagcgtcg
     4921 ctaatgtccc gtaactggca atctcataat gctcgacttt ctgtgctgcg gcaatcagtg
     4981 cggcatcacg cacttcgttt ttctcggtac tttcgatgac ctcattagct tcttcaataa
     5041 gaccttccat tgccacacat ttcatgcgct taattttcag attcgattct gattccacaa
     5101 cttgatcaat acgttcaatc tgtccatatg tttcctcgag gtgcgcatga aaagcctgac
     5161 ttaatttttc gtttgatgtt gctcttgcga gttttgccag tgcccgggtt aattgttttt
     5221 ctgcgctgta ggtatctgaa agcaggtgaa taaatacatc ttcaatggtc ttcatattca
     5281 tatttttctc cagtgaaatc actgcgagcc atacggcccg cagatgcaat tatctttatc
     5341 agctgatatt aataattaac ggtgaatcag gatttgcctg atttattacc accgctttgt
     5401 tgaccgcctt ttttacccgc ttcagatgcg cgttgcggat catttttaaa attaccgccg
     5461 ctatgctgac cgcctttacg gccagcgtcg gatgcctttt cacggtcttc ggcgaaattt
     5521 cctgaaccac cacgatgttc ggccatgtta tttctcccgt tgcgttgcat tgtttcatta
     5581 atatgggtgt tgtgtgtcga cactcattaa aattagtcgc taatga
//
LOCUS       DAZGQL010000182         5566 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.183, whole genome shotgun sequence.
ACCESSION   DAZGQL010000182 DAZGQL010000000
VERSION     DAZGQL010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5566
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.183"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            87..343
                     /locus_tag="ACHKIR_004910"
                     /pseudo
     CDS             87..343
                     /locus_tag="ACHKIR_004910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001632353.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(365..636)
                     /locus_tag="ACHKIR_004911"
                     /pseudo
     CDS             complement(365..636)
                     /locus_tag="ACHKIR_004911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206187.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(633..2393)
                     /locus_tag="ACHKIR_004912"
     CDS             complement(633..2393)
                     /locus_tag="ACHKIR_004912"
                     /EC_number="2.1.1.-"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HFO9992532.1"
                     /translation="MTNSRIPDERQFLTSDNTELFYRHWPAQEASSTPKVVVLFHRGH
                     EHSGRLQHIVDELAMPDTAFYAMDARGHGRSPGLRGYSPSLVRSVQDVDEFVRFAAAD
                     SQAGMTDVVVVAQSVGAVLAATWAHDYAPDIRGLVLASPAFKVKLYVPFARQGLELMY
                     RLRGLFFVNSYVKGKYLTHDPERVASFNKDPRITRPIAVNILLDLYKTAERVVSDASA
                     ITLPVQLLVSGEDYVVHRQPQLDFYQNLRNPQKELHVLPGFYHDTLGEKERHLAFDRM
                     RSFIDTLYARPVQRFDYSQEDRWSPSADTWRILSGGPALYSLDDISYRCLRYGMKVLG
                     TQSTGVRLGFETGFDSGSTLDYIYRNQPQGSTSLGRLIDKNYLNSVGWKGIRQRKIHL
                     QMLIQQAVARLTEQGSSVRIVDIAAGHGRYVLDALQGETAVSEILLRDYSELNVAKGQ
                     EMIASRGMAGRARFEQGDAFNRNELAALEPRPTLGIVSGLYELFPENGPVRDSLAGLA
                     AAIEPGGILIYTCQPWHPQLKTIAWSLTSHKNGKAWVMRVRTQGEMDALVRDAGFEKC
                     TQLIDEMGIFTVSMAVRRQP"
     gene            complement(2386..3027)
                     /locus_tag="ACHKIR_004913"
     CDS             complement(2386..3027)
                     /locus_tag="ACHKIR_004913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFO9992533.1"
                     /translation="MMTRLLRTLFTLCIVWPVIWLWLGLRVRHRERLPKHGPSIIVAN
                     HNSHMDVFALLSLFPLRYQASVHPVAAADYFLRDKRMAWFALKILNIIPISRKGGYTD
                     PLAQCEQALREGKTLILFPEGTRGEPGVLAPLKSGVWHLSQRMPEVPIIPVGLRGTER
                     VMAKGNRIPLPLFIDVNIGDALSTHPEKKTFMDDLEQRLTALLQPSQGNHPHD"
     gene            complement(3031..3948)
                     /locus_tag="ACHKIR_004914"
     CDS             complement(3031..3948)
                     /locus_tag="ACHKIR_004914"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016156609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFO9992534.1"
                     /translation="MTLLEKSLIVIFSLLLIATVINGLLVWLRPSKDWRELTLRIRTW
                     WVIIVLFSFALLSPSWLALTFFALVSFMALKEFLTLVPSRQSDRMPLLWMFIAIPLNY
                     WLIGIGWYGMFVVFIPVYVFLFLPARMVIAGDTQGFLRTVSQLHWSLMTTVFAFSHVA
                     FLLVLPADGMQTSALLVLFLVGLTEFNDIAQYLWGKSLGRIKVIPKVSPNKTLEGLLG
                     GVATTALAATVLGPLLTPLSWPMALLAGVIIGVTGFCGDVVMSAIKRDIGVKDSGTLL
                     PGHGGILDRLDSLIFTAPVFFHFVRYFCY"
     gene            complement(3945..4550)
                     /locus_tag="ACHKIR_004915"
     CDS             complement(3945..4550)
                     /locus_tag="ACHKIR_004915"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015730493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFO9992535.1"
                     /translation="MTLYQIKPSFQALLRPLMFWLNRRGVTANHITLCAMVLSLITGV
                     VLIIHPQPGLFVLLPVVLFVRMALNALDGMLARECNQRSRLGAILNETGDVISDISLY
                     LPFMLLPQSSIPLVLIVVFCALMTEFCGVLAQTINGVRSYAGPLGKSDRALVFGAWGL
                     SLSIWPQIVAWNNVIWGVATLLLLWTIFNRCRSALREEGVK"
     gene            complement(4608..4793)
                     /locus_tag="ACHKIR_004916"
     CDS             complement(4608..4793)
                     /locus_tag="ACHKIR_004916"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992536.1"
                     /translation="MLTQLHRVLYRCPGHYVYGSATQSTGTGTGSLASRNTSQSPLPA
                     QQAGFPRPELYSLSVCQ"
     gene            complement(5034..>5566)
                     /locus_tag="ACHKIR_004917"
     CDS             complement(5034..>5566)
                     /locus_tag="ACHKIR_004917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO9992537.1"
                     /translation="SVKINSPLHFILLLTGAMAGSIMVALPDINDRIDNITGYILIVA
                     GVLSASFYVVISSRLIVNFEPVTLLTGQLTFSFILACFFIVLTGTSLQLPIESVFLVF
                     LSGILQYFLAFCFYLYSLRWIPVHIAGAMLYFIPLVALLLSWFFLQEDISFIQGLGVF
                     ITILCVYILNKKYAIE"
BASE COUNT         1526 a         1475 c         1352 g         1213 t
ORIGIN      
        1 acagtctatc tctgcctttg cttcatggga tgttctttgt atattttact tgctgtttaa
       61 gtatgtatat aaggagtgca ttcattatgc caatgaagct tgtaaagaag aatccccgga
      121 gcaaagtgat tcgggagctt agctgtgagc aagagatggc agtaaaggcg gtctgcggac
      181 tgaaaaaaca gcaactacgg cactacatgc cttggccaat gatcttctcc gggaaatgcg
      241 tgagtatgat gtatggttac agtgtgactg tattccggga gattcgccga ccatgaattt
      301 tgctgctctt aaaaacaaca cgggaacttt ttatctgtcc agtttggata tagataatta
      361 ttgtactaat aagggaagcc aacaccagcc gacagaccag acgccgctgc tcattcagtg
      421 aaacgcagat aaacagtaaa gcaaatccag actccagtac ggaataatcg tcagcggtat
      481 taacgggatc cggtgctccc agtcaaacac caggctgctg atgttttctc tcccggcggt
      541 aaactgattc gtcatgccat aagtaagaaa gaagaatggg gccagcacca ttagccagcc
      601 gattccctga ataatcaccg cacgccgcgt tgtcatggtt gacgtcgtac cgccatagag
      661 accgtgaaga tgcccatttc atcaattagt tgcgtgcatt tctcaaaacc ggcgtcacgg
      721 accagcgcgt ccatctcgcc ctgagtacgt acgcgcatta cccaggcttt gccatttttg
      781 tgactggtga gtgaccaggc gatggttttt aactgagggt gccagggctg acaggtgtag
      841 atcagaatgc ctcccggttc aatcgctgca gccagcccgg caagcgaatc tcgtaccggc
      901 ccgttttccg ggaacagttc atacagaccg gacacgatcc ccagcgtcgg acgcggttcc
      961 agtgccgcca gttcattacg attaaaggca tccccctgct caaatcgcgc acggcccgcc
     1021 ataccgcgag aggctatcat ctcctgacct ttcgccacgt ttaattcgct gtaatcgcgc
     1081 agaaggatct cgcttaccgc cgtttcgcct tgcagggcat caagcacata gcgtccatgc
     1141 ccggcggcaa tatccacgat ccggaccgag ctaccctgct ctgttaaccg agcaacagcc
     1201 tgctgaatta acatctgcag atgaatttta cgctggcgga tccccttcca gcccacgcta
     1261 ttcaggtagt ttttatcaat gaggcgtccc aacgaggtgc tgccctgagg ctggttgcga
     1321 tagatgtagt ctagggtact gccggaatcg aaaccagtct caaatcccag acgcaccccc
     1381 gtggactgcg tgcccagcac tttcatcccg tagcgcaaac agcggtaaga gatatcgtcc
     1441 agcgaataca gcgcagggcc accgctcagt atgcgccagg tatcggcgct ggggctccag
     1501 cgatcttcct ggctgtaatc aaaacgctgc acgggacggg catacaaggt atcaataaaa
     1561 ctgcgcatcc tgtcaaatgc cagatgccgc tctttttcgc ctaaggtgtc atgataaaat
     1621 cccggcagta catgcagttc tttctgcgga tttcgcaggt tctgataaaa atcaagctgc
     1681 ggctggcggt gcacaacgta atcttccccg gagaccagca actgcacagg tagcgtaatg
     1741 gcgctggcat cgctgacgac tcgctcagcc gttttgtaga gatcaagcag aatattcaca
     1801 gcaattggtc gggtaatccg cggatcctta ttgaagcttg ctacccgctc gggatcgtgc
     1861 gtcagatact tgcctttcac atacgagttg acgaagaaaa gaccgcgcag gcgatacatc
     1921 aattccagcc cctggcgagc aaacggcaca tacaatttga ccttaaacgc cggtgatgcc
     1981 aacaccaggc cacgaatgtc cggggcataa tcatgcgccc aggttgccgc cagaaccgcc
     2041 ccgacgcttt gcgccacgac gaccacatcc gtcatcccag cttggctatc tgcggcggcg
     2101 aaacgcacaa actcgtcaac atcctgcacc gaacgcacga gcgacggact gtaaccacgc
     2161 agacccggtg agcgaccatg acctcgggca tccatggcat aaaacgctgt atccggcatc
     2221 gccagctcat caacaatgtg ttgcaaacgc ccggagtgct catgcccacg atgaaacagg
     2281 accaccactt ttggcgttga actggcttct tgtgctggcc agtggcgata aaacagctct
     2341 gtattatcac tggtcagaaa ctgccgttca tcgggtatgc gggaattagt catggggatg
     2401 atttccttgc gagggttgca aaagcgccgt tagtctttgc tccaggtcgt ccataaacgt
     2461 tttcttctcc ggatgcgtac ttagcgcatc gccaatattt acatcaataa acagcggcaa
     2521 agggatacga ttacctttcg ccatcacacg ctcagtaccg cgcaacccaa cggggataat
     2581 gggcacctcc ggcatgcgct gactcaaatg ccacactccg gatttcaatg gcgcaagtac
     2641 gcctggctca ccgcgcgtcc cttccggaaa caggatcagt gttttacctt cacgcagcgc
     2701 ctgttcacat tgcgccagcg gatcggtata accgcccttt cttgaaatag gaatgatgtt
     2761 aaggatcttc agcgcaaacc aagccatgcg tttatcccgc agaaaataat ccgcggcagc
     2821 aaccggatga acagaagcct ggtaacgcag cggaaaaagc gaaagcaagg cgaaaacatc
     2881 catatggctg ttgtgatttg ccacgattat cgaaggcccg tgcttcggca gtcgctcgcg
     2941 gtggcggaca cgcagaccaa gccacagcca gatgaccggc cagacgatac acagcgtaaa
     3001 cagggtacgg agtagtcgtg tcatcatagg ttagtagcag aaatagcgaa caaaatggaa
     3061 gaagaccggt gcggtaaaaa tcagcgaatc cagccgatca aggatgcctc catggccggg
     3121 tagcagcgta ccactgtctt tgacgccgat atcacgcttg attgccgaca tcaccacgtc
     3181 gccgcaaaag ccagtaacgc cgataataac cccggctaac agtgccatcg gccagctcag
     3241 gggagtcaac agtggcccca ggactgttgc cgccagcgcc gtcgttgcca ccccacccag
     3301 caatccctcc agggttttgt ttgggctgac cttcggtatg acttttatac gaccaagcga
     3361 cttgccccac aggtattgcg cgatgtcgtt aaattcggtc aggccgacca aaaacagcac
     3421 caacagcgcg ctggtttgca ttccatccgc cggtaacacc agtaaaaacg cgacatggct
     3481 gaaggcaaat acggtggtca tcagactcca gtgcaactgg gaaacggtac gtaaaaagcc
     3541 ctgtgtatcc ccagcaatca ccattcgcgc cggtaaaaaa aggaacacgt agaccgggat
     3601 aaacaccaca aacatgccgt accagccgat acctatcagc cagtagttaa ggggaattgc
     3661 aataaacatc cataacagcg gcatgcggtc ggactggcgg gaaggcacca gcgtcaaaaa
     3721 ctcttttagc gccataaagc tgaccagcgc gaagaacgtc agtgccagcc agctaggact
     3781 taatagtgcg aaggaaaaca ggacgataat cacccaccag gtgcgaatac gtaatgtcag
     3841 ctcgcgccag tctttacttg gacgcagcca caccagcaac ccgttgatta cggtggcaat
     3901 caacagcagg gaaaaaataa cgatcagtga tttttcaagt agcgtcattt cacaccctct
     3961 tcacgcaatg cgctgcgaca gcgattaaaa atcgtccata acaacaacag tgttgcgaca
     4021 ccccatatga cattattcca ggcgacaatc tgcggccaga tcgacagtga cagcccccat
     4081 gcaccaaaca ccagcgctct gtcgcttttg cccagcggac cggcatagct gcgcacgccg
     4141 ttaatcgttt gcgccagcac gccgcaaaac tcggtcatca gcgcacaaaa caccacgatc
     4201 agcaccagcg gtatactgct ctgcgggagt agcataaagg gcagataaag cgagatgtcg
     4261 gaaattacat cacccgtctc attgaggatg gcccccagac gcgatctttg attacattca
     4321 cgcgccagca taccatcgag cgcattgagc gccattcgta caaacaacac cacgggcaga
     4381 aggacgaata atccgggctg aggatgaata atgagcacca cgccggtgat aagggaaagc
     4441 accatcgcgc acagtgtaat gtgattagcg gtgacgccgc gcctgttcag ccagaacatc
     4501 agcggtctta acagagcctg aaacgagggc tttatctggt aaagggtcat catcactcct
     4561 taataagcat atttgacggg gttgagtctg gccttttatt ggggctgtca ctggcacact
     4621 gaaagtgaat acagttccgg gcgcggaaat cctgcctgct gcgccggtaa aggcgactga
     4681 ctggtattac gggaggccag agagccagta cctgtgccgg tagactgtgt ggctgaacca
     4741 taaacatagt gtcctggaca acggtacagc actctgtgca gctgtgtcag catgcgcttt
     4801 tctttaatta tgcccagtca tgtgcggagg caaggatttc aaaatatccg ttacatttgt
     4861 tgcatgatgc cttatcgctg gtccgggcta ttgctttact tcttttaaat cagctctgat
     4921 gaacattttt taccacttta tcgcattaag gttatgtcat tcatcgtctg gcatcacgtt
     4981 tttggttatg gtgtcagggt gtaccctacc gcgataacgc aaccctaata aagtcattcg
     5041 atggcatatt tcttattgag aatatatacg cataaaatag ttataaaaac gcctagtccc
     5101 tgaataaatg atatatcttc ctgcaggaag aaccaggaca acagcaatgc taccagagga
     5161 ataaaataga gcattgctcc tgctatgtga acgggaatcc agcgtaggga ataaagatag
     5221 aaacaaaaag ccaaaaaata ttgtaaaatt cctgacaaaa acacaagaaa tacagattct
     5281 attggcagtt gcagggatgt gccagttaga acaataaaaa aacaagcaag aataaaagag
     5341 aaagtcaact gcccggttaa caacgtaaca ggctcaaaat tcacaataag tcgtgatgag
     5401 ataacaacat aaaaggatgc tgagagtaca cctgcaacaa taagtatata tcctgtaata
     5461 ttatcaatgc ggtcattgat atcaggtagg gcaaccatta tactgcctgc cattgctcca
     5521 gtaagcaata atataaagtg taatggtgag ttaattttaa cggaaa
//
LOCUS       DAZGQL010000183         5525 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.184, whole genome shotgun sequence.
ACCESSION   DAZGQL010000183 DAZGQL010000000
VERSION     DAZGQL010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5525
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.184"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..459)
                     /locus_tag="ACHKIR_004918"
     CDS             complement(<1..459)
                     /locus_tag="ACHKIR_004918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020237521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992538.1"
                     /translation="MGHGTCKAAKSARHLIESKEKNRSSRVKVFSTHINLSHPAASWL
                     ATQTCANNIFNLLQNNNVIYLFENDNKIELRMGYDSFFSIETIDKINKENIKEIKLKN
                     EKTYVSLPNPTILITTTSTPERQFSISVLDDDNKENLPTDTAPCFTIGIPD"
     gene            complement(1052..1897)
                     /locus_tag="ACHKIR_004919"
     CDS             complement(1052..1897)
                     /locus_tag="ACHKIR_004919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001564112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFO9992539.1"
                     /translation="MSAITVSRPEVVTGHTDVICSTSVSHILAVRKTTLLQIDTLIRQ
                     LAEISVLTESIGGKTALDWAMKQDFRCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDAQARDTWYRSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVINVFRGLS
                     WNYKTNCPCKFGSKIIVNNLVRWDWWGFHLITGQQADRLADLERMLHLFSGKPIPDNR
                     ENITLRLDDHIQSVQGKECYEDEMFSIRYFKKGSAHITFKNQELVDRLNDIIARHYPR
                     MLPSL"
     gene            complement(1982..2179)
                     /locus_tag="ACHKIR_004920"
     CDS             complement(1982..2179)
                     /locus_tag="ACHKIR_004920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFO9992540.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAREDIHTLRQVQLLHQNR"
     gene            complement(2191..2679)
                     /locus_tag="ACHKIR_004921"
     CDS             complement(2191..2679)
                     /locus_tag="ACHKIR_004921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFO9992541.1"
                     /translation="MKLTLTLEADNVNVQALNMGRIVVDVDGVNLAELINVVCDNGHS
                     LRVVDESDRVSTDCTPPFAALTGIRCSTAHITETDNAWLYSLSHQTNDDCETEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLTRRYGVSLIHLDASAECLPGLPTF
                     NW"
     gene            complement(2676..3053)
                     /locus_tag="ACHKIR_004922"
     CDS             complement(2676..3053)
                     /locus_tag="ACHKIR_004922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFO9992542.1"
                     /translation="MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPEFSACTRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITQGKHPEAKQ"
     gene            complement(3100..3474)
                     /locus_tag="ACHKIR_004923"
     CDS             complement(3100..3474)
                     /locus_tag="ACHKIR_004923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFO9992543.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRNQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPAQAPATTS"
     gene            complement(3637..3858)
                     /locus_tag="ACHKIR_004924"
     CDS             complement(3637..3858)
                     /locus_tag="ACHKIR_004924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFO9992544.1"
                     /translation="MKIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSTEAYTGREVQ
                     DIPGVLAVFAERHKDSFGPYVRLMSVTLN"
     gene            complement(3921..4397)
                     /locus_tag="ACHKIR_004925"
     CDS             complement(3921..4397)
                     /locus_tag="ACHKIR_004925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFO9992545.1"
                     /translation="MQQLSFLPGEMTPGERSLILRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            complement(4413..4898)
                     /locus_tag="ACHKIR_004926"
     CDS             complement(4413..4898)
                     /locus_tag="ACHKIR_004926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO9992546.1"
                     /translation="MKTISHNSTTSSVSVTAASGNDQSQLVATPVPDEQRISFWPQHF
                     GLIPQWVTLEPRIFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDGENWVLFNA
                     MNGNRAEMSPEAAGIVACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            complement(4953..>5525)
                     /locus_tag="ACHKIR_004927"
     CDS             complement(4953..>5525)
                     /locus_tag="ACHKIR_004927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO9992547.1"
                     /translation="MLRLRRAGQITGQHVPEIILLNSHDGTSSYQMLPGYFRAICTNG
                     LVCGQSLGEVRVPHRGNVVDRVIEGAYEVVGVFDRIEEKRDAMQSLVLPPPARQALAQ
                     AALTYRYGDEHQPVTTADILTPRRREDYGKDLWSAYQTIQENMLKGGISGRSAKGKRI
                     HTHAIHSIDTDIKLNRALWVMAETLLESLR"
BASE COUNT         1343 a         1301 c         1435 g         1446 t
ORIGIN      
        1 atcaggaata ccaattgtaa aacaaggtgc ggtatcggta ggtagatttt ctttgttatc
       61 atcatccaga acagaaatag aaaactgtcg ttcaggtgtt gatgtcgtgg ttattaaaat
      121 ggttggatta ggaagagaaa catatgtttt ctcatttttt agttttattt ctttgatgtt
      181 ttctttgtta attttatcaa tcgtttcaat agaaaaaaaa gaatcatacc ccattcgcag
      241 ttcaatttta ttatcgtttt cgaataaata aatgacatta ttgttttgaa gtagattgaa
      301 tatattgtta gcgcaagttt gagttgctag ccaggatgcc gctggatgac tcaaattaat
      361 atgcgtgcta aagactttga cgcgactact tctgtttttc tctttacttt caattaaatg
      421 cctagcgctt ttggctgctt tacaagttcc atgccccaca cggcaaaaat aatctcctga
      481 aaggatactt acaatcagta ttacaccaat gacgacagaa aaaatagttc cgatgagttt
      541 gatgatgaca atccttgtgc agtgttattt tcaccatgga ttataatgtt atagtgaaat
      601 tattgcacat gtaatttttc tttgattgtt ttaacatcac atttatgcac ttcttcatct
      661 ttgtagcagg taaaagccat atatggatgg ctggtgtccg gtgcagataa aatgcaactt
      721 attcttgata gcttgaaaaa aagagaggtt tttttgagtg acggttgata gtcggtggca
      781 aggaagacgt tttcgtacac tcggcaagaa aaatgaatca ctctgtcata ctaccagata
      841 catcagcagg gtcaggttgc tcagaaataa gcaggatggg gatcactgaa gaggcatttt
      901 ggcgatcaca ggagtcgaat atgagtatta atatattaat tttaaatgat taatttgtgt
      961 tggataaatg agtaaccccc ataagaaccc ccaaataatt ttgcgtgagg gaatgaagct
     1021 aaaatgcaaa attgaattca ttcttagaac tttataatga aggtaacatt ctcggatagt
     1081 gtcttgcaat aatgtcattc agcctgtcaa ccagttcttg atttttgaac gtgatgtgcg
     1141 cggagccctt cttaaagtat ctgatgctga acatctcatc ttcatagcac tctttaccct
     1201 gaacagactg gatgtgatca tccagacgaa gggtgatgtt ttcccggttg tcggggatcg
     1261 gtttgccgct gaacaggtgt agcattcttt ccagatcggc aagtcgatct gcctgttgcc
     1321 cggtgataag atgaaacccc caccagtccc acctcaccag gttgttgacg ataattttac
     1381 tgccaaattt gcaggggcaa ttggttttgt aattccagct cagtcccctg aagacgttga
     1441 tcacccctcg ctcaaacacc tcatccttat tctggtgcag ttgttcaaat gtgctcagga
     1501 tattcgcctc gctgatttcc ggaaaattat catattccag tgaccggtac catgtatcac
     1561 gggcctgtgc atccattaag gaaagcatcc cggaacgttg catcaggtca cgccagattt
     1621 cacgatcgag attgcgtgtg atagctttca ttgcggtttc aggtttctcc atcagccagc
     1681 agccgcagcg aaaatcctgt ttcatggccc agtccagagc agttttacct ccaatacttt
     1741 ctgtcagtac cgagatttca gccagttgcc ggataagtgt gtcgatttgc agcagtgtag
     1801 tctttcgcac agccagaatg tgactgactg aggtggagca gataacgtcc gtatgcccgg
     1861 tgaccacttc cggacgggag actgtgattg cagacataat cgatattcct tttcgcagat
     1921 aaatgaaaac gccagccctg ttcaggctgg cgtcagtgga atgaagtctg gtgagtaaga
     1981 ctcaccggtt ctggtgcaga agctgcacct ggcgcagggt atggatatcc tccctcgcct
     2041 gttcaatgca gggcagcagt gctgccgaat ccgttttatc ctcatcgttg tcaaagataa
     2101 ttcccgattc gcagtcgata ttgtcctgca gccacgcaat cagaatatcc agtgctgttt
     2161 ccgtggttaa tgatttcatg ttgttcctgg ttaccagttg aaagtgggta aacccggcag
     2221 gcattcagcg ctggcatcca gatgaatgag actgacgcca taacgtcggg taagtgtgac
     2281 aaccagacga cggaacgttt ttgacaggcc caggcgctta agccgcagaa ccgggtatga
     2341 ccacgcatcg gtacgtaaca gatatccgct gcctgtaaaa tgaatccatt ctgtttcgca
     2401 gtcgtcatta gtctggtgtg acagcgagta cagccaggcg ttgtccgttt ccgtgatatg
     2461 tgcggtactg cagcgtatgc cggtaagggc agcaaatggt ggcgtgcagt ctgttgagac
     2521 ccggtcagat tcatcaacaa cacgaaggga gtggccattg tcgcagacca cgttaatcag
     2581 ttcagcgaga ttaacaccat cgacgtcaac gacaattcgt cccatgttca gcgcctgcac
     2641 gttaacgttg tctgcttcca gcgtcagagt cagtttcatt gtttcgcctc cggatgcttc
     2701 ccctgggtaa tgttatttac cgttctgtaa ttgtcgcggg tcatcaggcc ggtcgccctg
     2761 cgagcccgga ggatatcgat gctgtttatt aactgagaac gggtacaggc actgaattcc
     2821 ggctggtcgg tacgcaccag tgcgtatttt tcaacaagaa agttcactgc atcgcacagt
     2881 gaaatgccag cttcaatatg ctgctcaatc acacgttcat cggcaaacgg tgtgtcattc
     2941 agtgtgaggc cataatgctg gtccagcagt cggatgagca gtgtctgcca gatggtgacg
     3001 ggagacgggc agcgagatac ctcccgtaca tgtgtgtcgg ataatgtttt catcttaagt
     3061 tctctgtaac tggtagtgct aaaggtgaga gcttcggggt tatgaggtgg ttgcgggtgc
     3121 ctgtgccgga taaacagcca gataaacgta accacaggag ccgagggtgt cggcttcgca
     3181 ggtcagccct tttgcataca gcgtgacggt atgctgattt cggggattca gttcaccgct
     3241 ggtgagcatg agctccagtt gtttcatcag cagcggaaag gcctggtcca gatggtatgc
     3301 atctgcgtcg ctgaagcggc ctctgatacc ggcgcggtcg gcaaggtaat gcaggcggtt
     3361 accctcctgc accagacggg caccaaaaca gggcctcact gtgcagggaa gcccccacca
     3421 ggggcggtcg tgattgtcgt cgggatgagt tatcccggag agtttgtctg acacgataaa
     3481 atcctctctg aaaagtggtt aagaatggtt taacgatgac aggtcagccg gcgcgctgac
     3541 ctgcttcggt aaaggtgtat tcgttgatga gcagggcctc atccacagcg gcgtctgagt
     3601 ccatctggtc gtactcgttt tccgggtgac ggaacatcag ttcagggtga cgctcatcag
     3661 ccggacatac gggccaaaac tgtccttatg gcgttcagca aacacggcca gcacaccggg
     3721 aatatcctgc acttcacgac cggtatacgc ttcagtgctg ccgtgccagc ggtatttgcc
     3781 ggtacagaaa ggaaaaagac gggatgccgg atgttgttgg tgaatacgca tggcttcacc
     3841 acgggtgatg attttcatag tgtgatacct ctgaagacag aagataaaag tgaaaacaag
     3901 cgtgatgtgg ttgtgacggg ttaaagcaga ccatgttcgg caaaggagaa aacctggttg
     3961 ccaccgacta tcagatggtc cggcacccgg atatccacca ggcccagtgc ctgtaccaga
     4021 cgttccgtga taaggcggtc tgccttgctg ggtgtgactt caccggacgg gtgattgtgc
     4081 gccagcacca cggcagcggc attgtggtac agggcgcgtt taatcacttc ccggggatgg
     4141 acttccgtgc ggttgatggt gccggtgaag agggtttcac cggcaatcaa ctgattctgg
     4201 ttattcagat acagcacccg gaattcttca cgctccagtc ccgccatgtt cagaatcagc
     4261 cattcccgtg ccgcacgggt ggaggtgaag gccacgccgg gttcatgaag atggcggtcc
     4321 agggttttca gggcccgcag aatgagactg cgctcgccgg gcgtcatctc tccgggcaga
     4381 aaggaaagtt gttgcattgt gcttctctcc attcagtcga tgatgcgcat aatggcgctg
     4441 cattccggat gctgcagggc gtaatcccgc aaccggtaat aatggaccgt catggcataa
     4501 cactccgtac gacaggcatg atgactgtac gtcatcagac aggcgacaat gccggcggct
     4561 tccgggctca tttcagcgcg gttaccgttc atggcattga acagtaccca gttttcgcca
     4621 tcatcgtcat ctggttcggg ggccataaat gcgccaccgt tgttcagtgt gtacagattc
     4681 cagataccac cgcagtagtc ttcgcaaaga cggtccatcc agccgaagat acggggctcc
     4741 agggtcaccc actgtggaat gaggccaaaa tgctgcggcc agaagctgat gcgctgttca
     4801 tcagggacgg gagtggcaac cagctgagac tggtcattcc ctgatgcagc ggttacggaa
     4861 acagaagatg tggtggaatt atgtgaaatg gttttcatga aatgactcct taaaggacaa
     4921 ataaatgcgt ggttttcagg gtaatggctg aatcagcgca ggctctccag cagcgtttcc
     4981 gccatcaccc acaatgcgcg gttgagctta atgtcagtgt caatgctgtg aatggcatgg
     5041 gtatggatac gttttccttt agcgctgcga cctgaaattc cacctttcag catattctcc
     5101 tgaatggtct gataagcact ccacaggtcc ttaccgtaat cctcccggcg tcgtggcgtc
     5161 agaatgtcgg cggtggtgac gggctgatgt tcgtcaccat aacggtaagt cagtgccgcc
     5221 tgtgccagcg cctggcgtgc cggtggcggc aggaccagcg actgcatggc atcacgcttt
     5281 tcctcaatcc ggtcaaacac gcccaccact tcgtaagcgc cttctatgac cctgtccacc
     5341 acgtttcccc ggtgtggcac ccggacttct cccagcgact gaccgcagac caggccattg
     5401 gtacaaatgg cacgaaaata tccgggtaac atctggtagc tggaggtacc gtcatgggag
     5461 ttgagcagaa taatttcagg cacatgctga ccggttatct gtccggcccg ccgcagacgc
     5521 agcat
//
LOCUS       DAZGQL010000184         5515 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.185, whole genome shotgun sequence.
ACCESSION   DAZGQL010000184 DAZGQL010000000
VERSION     DAZGQL010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5515
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.185"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            148..999
                     /locus_tag="ACHKIR_004928"
     CDS             148..999
                     /locus_tag="ACHKIR_004928"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001195265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFO9992548.1"
                     /translation="MQKVKLNNGIEMPLLGFGVFQMTDAAECERAVIDAINSGYRLID
                     TAASYQNEIQVGNALKQSGIARNELFVTTKLWLQDTSYEGAKAQFERSLNRLQLDYVD
                     LYLIHQPYGDVHGAWRAMEELQQAGKIRAIGVSNFHPDRLADLIAFNNVVPAVNQVEV
                     NPFNQQLQAVPWMQSRGIQPEAWAPFAEGKNGLFQHPVLTAIGEKYGKSVGQVVLRWI
                     YQRGIVSLAKSVRKERMEENINILDFELSPEDMLQITALDTATSAFFSHRDPAMVEWL
                     TGRKLDV"
     gene            1026..2015
                     /locus_tag="ACHKIR_004929"
     CDS             1026..2015
                     /locus_tag="ACHKIR_004929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFO9992549.1"
                     /translation="MQKRYLGKSGLEVSALGLGCMGLSHGYGPATDTRQAIELIRAAV
                     ERGVTFFDTAEVYGPYLNEEVVGEALKPFRDRVVIATKFGFTFGDDNKQQILNSRPEH
                     IREAVEGSLRRLKTDVIDLLYQHRVDPDVPIEDVAGTVKDLIAEGKVKHFGLSEAGAQ
                     TIRRAHAVQPVTALQSEYSMWWREPEQEILPLLEELGIGFVPFSPLGKGFLTGAIKPG
                     TTFGKDDYRSTVPRFAAQSIEANEKLVTLLGELAAEKGVTSAQIALAWLLAQKPWIVP
                     IPGTTKLHRLEENLAAADIVLSQKDTQQISEALETIKIVGERYSPEHQARVGR"
     gene            complement(2046..2939)
                     /locus_tag="ACHKIR_004930"
     CDS             complement(2046..2939)
                     /locus_tag="ACHKIR_004930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO9992550.1"
                     /translation="MLKENFNELQIFLVVARERSFTKAAGKLGVSQSALSHAIKALEE
                     RLNIRLLTRTTRSVAPTEAGERIIACLEPRIDELEQELESLIQLNGTPSGNIRLSAGE
                     HAARSLVWPKLKPFLREYPEINVELVVDNGFVDIVEGRFDAGIRLGENVDKDMVAVRI
                     GPDMRMAVVGAPAYFAANPAPETPHELQNHRCINMRLPTAGGLYHWEFEREGKPLRVK
                     VDGQLTCSPLPERIDAALSGFGIACVPEDMVQEYIESGKLIQVLQEWCPTFPGYYLYY
                     PSRKQHPPAFALLIDALRYTE"
     gene            3299..3499
                     /locus_tag="ACHKIR_004931"
                     /pseudo
     CDS             3299..3499
                     /locus_tag="ACHKIR_004931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308358.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
     gene            4022..4123
                     /gene="ykgR"
                     /locus_tag="ACHKIR_004932"
     CDS             4022..4123
                     /gene="ykgR"
                     /locus_tag="ACHKIR_004932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YkgR"
                     /protein_id="HFO9992551.1"
                     /translation="MKENKVQQISHKLINIVVFVAIVEYAYLFLHFY"
     gene            4488..4751
                     /gene="ykgM"
                     /locus_tag="ACHKIR_004933"
     CDS             4488..4751
                     /gene="ykgM"
                     /locus_tag="ACHKIR_004933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFO9992552.1"
                     /translation="MKPNIHPEYRTVVFHDTSVDEYFKIGSTIKTDREIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVSTKKGA"
     gene            4751..4891
                     /gene="ykgO"
                     /locus_tag="ACHKIR_004934"
     CDS             4751..4891
                     /gene="ykgO"
                     /locus_tag="ACHKIR_004934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HFO9992553.1"
                     /translation="MKVLNSLRTAKERHPDCQIVKRKGRLYVICKSNPRFKAVQGRKK
                     KR"
     gene            complement(4926..5151)
                     /gene="ykgL"
                     /locus_tag="ACHKIR_004935"
                     /pseudo
     CDS             complement(4926..5151)
                     /gene="ykgL"
                     /locus_tag="ACHKIR_004935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414829.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkgL"
     gene            5298..>5515
                     /locus_tag="ACHKIR_004936"
     CDS             5298..>5515
                     /locus_tag="ACHKIR_004936"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992554.1"
                     /translation="MQCPGKEAIVPVTTGSLRLTSLNLALGGLTGQMTYLHLFNFSSL
                     IGMNTHNHFVRHVNPLKHLTDWQIIFNY"
BASE COUNT         1557 a         1317 c         1197 g         1444 t
ORIGIN      
        1 ttccggggat atcgaacttg cacgactaat ctcaaccctc aaacagattt atgaattttg
       61 ttcataaccc ctgacaaatc ctgccgtcta atcgagctct tccctttacg ttatgcttct
      121 catgaacgat aacggaagga taacaccatg caaaaagtaa aactgaacaa cggtattgaa
      181 atgcccctgt tgggctttgg tgtatttcag atgaccgatg ccgccgaatg cgaaagagcg
      241 gtcattgacg ctattaacag cggttatcga ctgattgaca ccgccgcctc ttatcaaaat
      301 gaaatccagg tcgggaacgc actgaaacaa agcggtatcg cccgtaacga actctttgtc
      361 accacgaaac tctggttgca ggataccagt tacgaagggg ccaaagccca gttcgaacgc
      421 tctctgaatc gactgcaact tgattacgtt gacctgtact tgattcacca gccatatggc
      481 gatgtccacg gagcctggcg tgccatggaa gagctgcaac aggccggtaa aattcgcgct
      541 attggcgtca gtaacttcca tcctgaccga ctggcagacc ttatcgcttt taataacgtc
      601 gttccggcgg taaaccaggt tgaagttaac cctttcaatc agcagctaca ggccgttccc
      661 tggatgcaaa gtcgtggcat tcagccagaa gcctgggcac cgtttgcgga agggaaaaat
      721 ggtctgttcc agcatcctgt tctcacggca attggcgaga agtatggcaa aagcgtgggc
      781 caggttgttc tgcgctggat ctaccaacga ggcattgttt cactggcaaa atcagtgcga
      841 aaagaacgca tggaagagaa catcaacatt ctcgattttg aactcagccc tgaagatatg
      901 ctgcaaatta ccgccctgga taccgcaacc agcgcgttct tctctcaccg cgatcccgca
      961 atggtggaat ggctgactgg ccgcaaactt gatgtttaag ccgcgataaa agaggtattc
     1021 attcaatgca aaaacgttat ctgggtaaat ccggactcga agtctccgcc cttgggctcg
     1081 gttgcatggg cttaagccac ggctacggcc cggcgaccga tacccgtcag gctatcgaac
     1141 ttattcgtgc tgcggttgaa cgtggcgtca ccttcttcga taccgccgaa gtgtatggtc
     1201 cgtatctgaa tgaagaagtg gtcggcgaag ccttaaaacc gtttcgtgac cgcgtggtaa
     1261 ttgccactaa attcggtttt accttcggcg atgataataa gcagcagatt ttaaacagcc
     1321 gtccggaaca tatccgtgaa gccgttgaag gatcattacg tcgtctgaaa acagatgtta
     1381 ttgacctgct gtatcaacac cgtgttgatc cagatgttcc tattgaagat gttgcaggaa
     1441 cggtgaaaga cctcatcgca gaaggtaagg tcaaacattt cggcctgtct gaagcaggag
     1501 cacaaaccat tcgtcgtgca catgcggtac aacctgtcac cgccctgcaa agcgaatatt
     1561 ccatgtggtg gcgtgagcct gaacaggaaa ttctgccgtt gctggaggaa ctgggaattg
     1621 gttttgtccc cttcagccca ctaggtaaag gtttcctgac aggggcgatt aagccgggaa
     1681 ccactttcgg caaagatgat taccgcagca ccgtaccgcg tttcgcagca caatccattg
     1741 aagccaatga aaaactggtc accttgttag gtgaactggc tgcggagaaa ggggtaacgt
     1801 ctgcacaaat cgcactggca tggctattag cacaaaagcc gtggattgtt cccatccctg
     1861 gcaccactaa actgcaccgg ctggaagaaa acctggccgc tgctgacatt gttctttcgc
     1921 agaaagatac gcagcagata agcgaggcgc tggaaacaat taaaatcgtc ggtgaacgtt
     1981 actcgcctga acatcaggca cgcgtaggcc gctaataccc aaacgggtcc aggataaagg
     2041 acccgttatt cggtgtagcg aagcgcatcg atcaacagcg caaaagctgg cggatgctgc
     2101 ttgcggctgg ggtagtaaag ataataccca ggaaaagttg gacaccactc ctgtaacacc
     2161 tgaataagct tgcctgactc tatatactcc tgaaccatgt cttcagggac acaagcgatc
     2221 ccaaaccctg ataacgcggc atcaattctt tctggcaatg gactacacgt cagttgccca
     2281 tcaaccttaa cgcgaagcgg tttcccttct cgctcgaact cccagtggta aagcccaccg
     2341 gcagttggca aacgcatgtt gatgcagcga tgattttgta gctcgtgagg cgtttcaggc
     2401 gcagggtttg cagcaaaata cgccggagct cccaccacag ccatccgcat atctggtcca
     2461 atcctgactg caaccatatc tttatcaaca ttttcaccca gtcgtatccc ggcatcaaaa
     2521 cggccttcaa caatatcgac aaaaccgtta tccaccacca gttcgacatt gatttcggga
     2581 tactctctga gaaaaggttt tagcttcggc cagaccagac ttcgcgcggc atgttccccg
     2641 gcagataacc gaatattccc ggagggcgtg ccgttcaatt gaataagtga ttccagttcc
     2701 tgttcaagct cgtcaatacg cggttcaagg caggcaatga ttctctcccc cgcttctgtg
     2761 ggagccacac tgcgggtagt ccgtgtcagg aggcggatat tgagcctttc ctccagagcc
     2821 tttattgcat ggctaagtgc agactgagag acgccgagtt tgcccgctgc tttggtaaaa
     2881 cttcgctctc ttgccaccac aagaaagatt tgcagttcgt tgaagttttc tttgagcatt
     2941 ggcgatttcc ttgtagaagc atcctcttca cacacactca tgaagggggc atagatgcta
     3001 ttattttaac cacattatta gtaacaatga taatctggct gtatcgaaaa taattctaaa
     3061 ctttcaggag atttattatg aataaacgta ccctcacgat tgtgccattc ttcttaccga
     3121 tattctgaca cacatcatta actttctatt tttactcttt tctccaccat aaaatcaata
     3181 aaagcactaa cagtaattat aaataaaaac aaagccaaaa atcttggttc caaagctggt
     3241 aacaactaac cagtttaaat atacacatca aaatcagata aacatttgag gtattgtcat
     3301 gacaaataaa cacctatcac tgttttctcc ttttatgctg acagaaaaaa taaagccatg
     3361 taatcgtatc gttatggctc ctatgacgac ctggtcagct aatcctgatg gaaccatctc
     3421 tgagcaagaa ctcgaatatt ataaacgtcg ctcacaaaat attggtttag taattacagg
     3481 atatacctat gtcacgccaa ggaaattcag acatcgaaag gaactggctg gtttccgtta
     3541 atggattaaa tatcgattac tctcccgaga gtaaaactat caaatcaatg agtaaccgat
     3601 acagcctcgc aaattctttc agataattaa cccgtcactg cggcacgttc tctcgtaagg
     3661 cgtgcctgca gtgtaatcca gtatgtatcc ggcatagttg acggtttaat agactcaatt
     3721 cctctccctg gttacctaca acgatatctt gtataacacc ccaggagata cgacgttacg
     3781 atgagatgca atatgctcct cagcgccaat caacgccagg actgggccag tcttgagatc
     3841 agactcattc ccgtaactga tttcaggcga catatatcaa tgacgttggg gcaataaaag
     3901 atagccgatg taatacatct attcaaattt gtgatacatg tcaaaaaatt ggttgaccaa
     3961 actcgttatt tatataaggg cacttacgaa gtgcactctt ttttaaagcg aggaagtacc
     4021 aatgaaagag aataaagtac agcaaatcag tcataaactg attaatatcg ttgtttttgt
     4081 cgcaattgta gaatacgcct atttatttct ccatttctat taataacggc aataaactgt
     4141 tcacttcagt gatatttaaa atatgcatcc tctccctttt ttgtaagtaa ttattatatc
     4201 cgtgggagag gaatacacat tgtcaggtaa tcaatcatgc tgcaataaat catcggccag
     4261 taaagtggag atagcctcca ttctcgaaaa atccatactc tcagcgaaac catcatcaat
     4321 cactcatcca ggcgtttatg ggagcgtcgc caatggctgc taacaatgcc agacttcccc
     4381 gttgcggaaa ttccacatcc cacaaatagt cacagtgatt gggtgttgaa atgatccgga
     4441 tgagcatgta tctttacggt tatgttataa cataacaggt aaaaatgatg aaacccaata
     4501 tccatcctga gtatcgtact gtggtgttcc acgacaccag cgttgatgag tactttaaaa
     4561 tcggctcgac tatcaaaaca gaccgtgaga ttgagctgga tggcgtaacg tatccgtacg
     4621 taacaattga tgtctcttcg aaatcgcacc cgttctatac agggaagctg agaacagtgg
     4681 catcagaagg aaatgttgca cggttcaccc aacgttttgg tcgttttgtt agcacgaaaa
     4741 agggggcatg atgaaagtcc ttaactctct gcgtaccgca aaagaacgcc atccagactg
     4801 tcagattgtg aagcgaaaag gacggctgta tgtgatttgt aaatctaatc cacgttttaa
     4861 ggccgttcag ggtcgtaaga aaaaacgttg attcaaaatt cgacgaatta acgatatttg
     4921 tctgattaat aatcataccg gattaatgtt ggtgtattta ttacaccagc attaattttc
     4981 ctggggatgt atcttcctgt taatttaagg tcaactgcct gacgtttctc tccgaatatt
     5041 ccattatatt aatgttgact tgttgaccag ctgcgccctg tatgctggca tcaactctat
     5101 tttagactga acacgccact cagtttcctc cctttgcgac gcagcctgca tttacactca
     5161 aactgttaag atgataaatg tggtaaatct gttggtactg acataaaaac gtttacgcca
     5221 caggaacggc cagatccatc ggtaacccca tcgccgacgt tcgagtgcca gttagagtaa
     5281 cgcgtacaga tgactgaatg cagtgccctg gcaaagaggc catcgttcct gtgacaactg
     5341 gcagtcttcg tttaacttca cttaatttgg ctcttggggg gcttaccgga cagatgacgt
     5401 acttacacct gtttaatttt tcatcactta ttgggatgaa cacccacaac cattttgtgc
     5461 ggcatgttaa tccattaaaa caccttactg attggcaaat catctttaat tatga
//
LOCUS       DAZGQL010000185         5498 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.186, whole genome shotgun sequence.
ACCESSION   DAZGQL010000185 DAZGQL010000000
VERSION     DAZGQL010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5498
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.186"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            3..182
                     /locus_tag="ACHKIR_004937"
     CDS             3..182
                     /locus_tag="ACHKIR_004937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004920230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HFO9992555.1"
                     /translation="MIAFLLPWLTFFTIGRPLTGIFCLVLQITLLGWIPATIWAVYAL
                     SQYKTEQKIREATGK"
     gene            326..1171
                     /gene="mscS"
                     /locus_tag="ACHKIR_004938"
     CDS             326..1171
                     /gene="mscS"
                     /locus_tag="ACHKIR_004938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HFO9992556.1"
                     /translation="MVRNQDMLPGYAANLVAAVVIIIGGIAARMISGVLNNLMLGRNI
                     DATVALFLSALIRHAIIAFTLIAALGRVGVQTASVIAVLGAAGLAVGLALQGSLSNLA
                     AGILLVMFRPFRAGEYADLGGVAGTVQNVHIFSTTMRTLDGKIVVIPNGKIIAGEIVN
                     FSREPERRNEFIISVSYDADIDCVKQVLTDIVMSEERVLKNREITVRLNEPGESSMNF
                     VVRVWSRRDDLQSVYWDVLERIKREFDSEGISFPYPQMDIHLVRSGKQAESVTHNSRS
                     VSSRQ"
     gene            1297..2517
                     /locus_tag="ACHKIR_004939"
     CDS             1297..2517
                     /locus_tag="ACHKIR_004939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001124813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HFO9992557.1"
                     /translation="MPKKIMTGIDVFDAAMERIEWVFDTFSSVCLSFSGGKDSTVLFH
                     LMANIARRKKRRFSVLFVDWEAQYQCTIEHVTRLREMYDDITDTFFWVALPLTTVNGV
                     SQVQPEWVCWEDGAEWVRQPPEDAITRASFFPFYQNAMTFEEFIPAFNHWFAREKGMV
                     AAILTGVRADESLNRFMGLVSGRKLRYAEDKPWTTASAEGFYYTMYPLYDWKTRDVWI
                     YHARTRAAYNSLYDLMYRAGVPLRNMRVCEPFGPEQRKGLWLYHVLEPDTWGRMCARV
                     SGAVGGALYANESGAYFALRKQITKPVHHTWQSYAMFLLESMPEKTAEHYKNKIAVYL
                     RWWQSHGWPDGIPDEQDNDQGCKDIPSWRRICKTLLKNDFWCRMLAFSPNKPRHYERY
                     LARMREKRKEWGIL"
     gene            2747..4492
                     /locus_tag="ACHKIR_004940"
     CDS             2747..4492
                     /locus_tag="ACHKIR_004940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001361889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFO9992558.1"
                     /translation="MTAGEKRVASRLEAFMSNECLVWYDIPVGRKNRHPDFVIIDPEN
                     GLVFLEVKDWTVHTLHQVNHEQVILETNGILKSEINPLLQVRRYACDTVNALPADSRL
                     RQNDGQYKGRLNLAWAYGVVFTRITRQQLKALAGNDENAVERIFPSAQTICQDDMTQS
                     VLPDIFRQKIAGMFTMGFRTRVTPQVRDILRAHLFPEMTVRQHSQIKIMDIQQEILAR
                     NIGDGHRVIHGVAGSGKTMILLFRCLYLAETTPGKILVLCYNITLASYLRECIESRGL
                     KSRVTVFHFHSWCASMIKRHGIQVTAGGKDYPEKCFSALEDAVNSGTITDTGYDAVLV
                     DEGHDFESRWLALIARLFDNASRSLLLMYDDAQSIYRRERALNFSLASVGIQAQGRTS
                     VLPVNYRNTKRILHFAYAFSREYFEKHQNREIPFVQPQAGGEEGTEPEILRCASESDE
                     AVQVVGWLEKRYTLCGHWSDMAVLCPAEFSVKHLKEVMTQRGIPYATCFDSEGKKKYS
                     RRKDVVHLLTYQSSKGLEFPYVAVINASFVHSGAADESEVIPALYVAFTRATRELLVT
                     CYRENSISRHLEDFV"
     gene            complement(4674..5303)
                     /locus_tag="ACHKIR_004941"
     CDS             complement(4674..5303)
                     /locus_tag="ACHKIR_004941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFO9992559.1"
                     /translation="MSIEQIINEINVYLNSLKKEDERIDAINKIKIELHRSSPFYAEP
                     VDCVIWIKNNLIEANDYNPNVMAPSEKRLLTHSLLVDGFTQPVVTLAQKGKYTVIDGF
                     HRQMLGKSTTKLEKKLKGYLPVTCIKHDNNFKAYSIASTIRHNRARGKHQIDALSDIV
                     RDLSRMGWDDLRIGSELGMDTDEVLRLKQISGLTELFEEENFSPAWTIK"
     gene            complement(5293..>5498)
                     /locus_tag="ACHKIR_004942"
     CDS             complement(5293..>5498)
                     /locus_tag="ACHKIR_004942"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992560.1"
                     /translation="KENIIPSIIYLKITSFIMRSILSPPAPKIKTQNNLTTYILTQSR
                     ERRTTHLKTRKIYKLKEIETHEH"
BASE COUNT         1369 a         1045 c         1406 g         1678 t
ORIGIN      
        1 tgttgattgc ttttcttctt ccctggctga cttttttcac catcggccgg cctctgacgg
       61 ggattttttg tctggtgtta cagataacgc tgctgggctg gataccggca acaatatggg
      121 ccgtgtatgc gctttcgcag tataagacgg aacagaaaat ccgcgaagcc acggggaaat
      181 gatgactgca gaagagcagg ttgtcatacc ggtgtatatg atgcgggata aaggcccgga
      241 gattcctgga aataaaatgt tccggggaat ataaaacagg gaaaaaccgg atgtgatgga
      301 cgcttctggc gaggcggttt catgaattgt ccgtaatcag gatatgttgc ccggttacgc
      361 cgcgaatctt gtggcagcag tggttattat tatcgggggt atcgctgcac ggatgatttc
      421 tggtgtgctg aataatctga tgctgggcag gaatattgat gccacagtgg cgctgttcct
      481 ttccgcgctg atacgtcatg ccattatagc ctttacgctg attgctgccc tgggacgtgt
      541 gggggttcag acagcttccg taattgcagt cctgggggct gcaggtcttg ctgttggact
      601 tgccctgcag gggtcattgt caaaccttgc agctggtatt cttctcgtga tgttccgtcc
      661 tttcagggct ggtgaatatg ctgacctggg tggtgttgcc gggactgtgc agaatgtaca
      721 tatattctca accacgatgc gtacactgga tggtaaaatt gttgtcattc cgaacgggaa
      781 gattattgcc ggagagattg ttaatttctc cagggagcct gaacgacgaa atgagtttat
      841 catcagtgtt tcatatgatg cagatattga ctgtgtgaaa caagtcctga cagatattgt
      901 gatgtcagaa gagagggttc tgaagaacag ggaaatcact gttcgcctga atgagcctgg
      961 ggagtcatcc atgaattttg tagtacgggt atggagcagg cgtgatgacc ttcagtctgt
     1021 atactgggat gtactggaga gaattaagcg ggagtttgat tcagaaggga taagttttcc
     1081 atatccacaa atggatatcc atcttgttcg ctccgggaag caggcagagt cggtaaccca
     1141 taacagtcgc tcagtcagtt ccagacagta aaatgaaaag cgcattttat ttgtgaaaga
     1201 tgaacagcct gaatctgtca ggctgttttc attgtgtttt tatggtcatt tttggtatat
     1261 gtactgtata taatgtcatc tgtacaagga tataaaatgc caaagaaaat aatgacaggg
     1321 attgatgttt ttgacgctgc catggagcgt attgaatggg tgtttgatac gttttcatca
     1381 gtttgtcttt ctttttccgg cggaaaagac tccactgtcc tgtttcacct gatggcgaat
     1441 attgcccgcc gaaaaaaacg ccgtttttct gtactgtttg ttgactggga ggctcagtat
     1501 cagtgtacca ttgagcatgt caccaggttg agggaaatgt acgatgatat cacagatact
     1561 tttttctggg tggcgctgcc tctgacgacc gttaatggag tgtctcaggt acagcctgag
     1621 tgggtatgtt gggaggacgg ggctgaatgg gttcgccagc caccagaaga tgccataacc
     1681 agagcgtctt tttttccttt ttatcagaat gcgatgacat ttgaagagtt tattccggca
     1741 tttaatcatt ggtttgcccg ggaaaaaggg atggttgctg cgattcttac tggtgttcgc
     1801 gctgatgagt cactgaatcg ttttatggga ctggtatccg gacgtaagct ccgttatgcg
     1861 gaggataaac cctggaccac ggcatcggct gagggatttt actacaccat gtatcctttg
     1921 tacgactgga aaacaagaga tgtatggatt taccatgcga gaacacgtgc tgcctataac
     1981 tctctttatg acctgatgta cagggctgga gttcctcttc gcaatatgcg ggtgtgtgag
     2041 ccttttggac cggagcaacg taaggggttg tggctttatc atgtactgga gccggataca
     2101 tggggcagaa tgtgtgctcg cgtatcaggt gctgtagggg gagcgctcta tgcaaatgaa
     2161 agtggtgctt attttgcttt gaggaaacag ataacaaaac cagttcacca tacatggcag
     2221 agttatgcaa tgtttctgct ggagtcaatg cctgagaaaa cggcggagca ttacaagaat
     2281 aagattgctg tttatcttcg gtggtggcag tctcatggat ggccggatgg catccctgat
     2341 gagcaggata atgaccaggg atgtaaagat attccctcct ggcgtcgcat ctgtaagacg
     2401 ttactgaaaa atgatttctg gtgtcggatg ctggcgttta gtccgaataa gccccgacat
     2461 tatgagcgct acctggccag aatgcgtgaa aaaaggaaag agtggggaat attgtaaatt
     2521 atatttaaac atcagaaata tgacggttaa tccaggatag tagctaaaaa aataagattc
     2581 cttgctgatt tatccctgtt tgatatgtaa aatctgaggg gcttataagc aacggatata
     2641 cgagtgctac aatatatgat aagttatata tattaaatgt tcatcatctg tcaggggctg
     2701 ttttatggct aaactgataa atatatcagg tgttaccgag gagcggatga ctgccgggga
     2761 aaagcgggtt gcatcccgtc ttgaggcatt tatgagtaat gagtgtctgg tctggtacga
     2821 tattccggtg ggacggaaaa accgacatcc tgattttgtc attattgacc cggagaatgg
     2881 tctggtattt cttgaagtca aggactggac cgttcacacg ttgcatcagg taaatcacga
     2941 acaggtcatt cttgaaacta acggtattct taaatcagaa ataaatccgc tgctacaggt
     3001 tcgtcgttat gcctgtgata ccgtaaatgc tttacctgca gactcacgtt tgcgacagaa
     3061 tgatggtcag tataaggggc gtctgaatct ggcctgggcg tatggtgttg tgtttacccg
     3121 tattacccgc cagcaactga aggctctggc tgggaatgat gaaaatgctg tggaaaggat
     3181 tttcccttca gcgcagacaa tctgtcagga tgatatgact cagtctgttt tacctgatat
     3241 attccgccaa aaaattgccg ggatgtttac gatgggattc agaacccggg tgacaccaca
     3301 ggttagagat attctgcgtg ctcatctgtt tcctgaaatg accgtcagac agcactcaca
     3361 aatcaaaata atggatattc agcaggagat tctggccaga aatattggtg atgggcatcg
     3421 tgttattcat ggggtagccg gctctggaaa aacaatgatt cttttgttcc gttgtcttta
     3481 cttggctgaa actacccctg gtaagattct ggtgctatgt tacaacatta ctctggccag
     3541 ctacctgcgg gaatgtattg agtccagggg actgaagtca cgggttacgg tattccattt
     3601 tcatagttgg tgcgcctcaa tgataaagcg ccatggaatt caggtgactg ctggaggaaa
     3661 ggattacccg gagaaatgct tcagtgcgct ggaagatgcc gtgaacagcg gaacaattac
     3721 tgatactgga tacgatgccg tacttgtgga tgaggggcat gattttgaga gtcgctggct
     3781 ggctctgata gcccgcctgt ttgataacgc gtcccgttca ctgctcctga tgtacgatga
     3841 cgcacagtcc atttacagac gggaacgggc tctgaacttt tcgcttgcca gtgtggggat
     3901 tcaggcgcag ggacgtactt ccgtcctgcc ggtaaattac cgtaatacga aacgcattct
     3961 gcactttgct tatgcattct cccgtgagta ttttgagaag catcagaacc gtgaaatacc
     4021 gtttgttcag ccacaggcgg gcggtgaaga gggcacagag ccggaaattc tgcgttgtgc
     4081 ttctgagagt gatgaggcag tgcaggttgt gggctggctg gagaaacgct atacactgtg
     4141 cggtcactgg agtgatatgg ctgttttgtg tcctgcagag ttttcagtga aacatctgaa
     4201 agaggtcatg acacaacgcg ggatacctta tgccacctgc tttgacagtg aaggtaagaa
     4261 gaaatattcg cgacgcaagg atgttgtgca tttactgacc taccagagca gtaagggact
     4321 ggagtttccg tatgttgcgg ttatcaatgc ttctttcgtt cactcaggtg ctgcggatga
     4381 gtctgaagtt attccggcgc tttatgttgc gttcacccgg gctacacggg aattactggt
     4441 gacctgttat cgtgaaaaca gtatcagtcg tcatcttgag gattttgtat aagttgtgtt
     4501 ttattattgc tgatatgtaa tcattcgttt tttgataatc taagatgggt atttagcgag
     4561 tattcagagt tgaattaata tcccccatgt ttttgatctg tcaatagctg tccattacat
     4621 ggggtgatca tatatcagtc ggtaattact ggataaagtc accccctaaa tgattacttt
     4681 attgtccatg ccggactaaa gttttcctcc tcaaatagtt ctgttaaccc acttatttgt
     4741 tttaatctca gtacctcatc tgtatccatg cccagttcac tacctattcg gagatcatcc
     4801 catcccattc gggaaagatc tcggacgata tcggagagcg cgtctatctg gtgctttcct
     4861 ctggccctgt tatgcctgat ggtcgaggcg atagagtaag ctttaaagtt attatcatgc
     4921 ttaatgcatg tgacaggtaa atatcctttt agttttttct ctagttttgt tgtgcttttc
     4981 ccaagcattt gtctgtgaaa tccgtcaata acggtgtatt tccccttttg agctaaggtg
     5041 acaacaggtt gagtgaatcc atcaaccaac agggagtgtg ttaataacct tttttcggag
     5101 ggagccatga cgttaggatt atagtcgttt gcttcaatta gattgttttt tatccatata
     5161 acacaatcaa caggttctgc gtaaaaagga ctactcctat gtaactcaat ctttatttta
     5221 ttaattgcat caatgcgttc atcttctttc ttaagagaat taaggtatac atttatttca
     5281 tttatgattt gttcaatgct catgtgtttc tatttctttt aatttgtata tttttcttgt
     5341 tttaagatga gttgttcttc tttctctact ttgtgttaat atataagttg taaggttgtt
     5401 ttgtgttttt atttttggtg ctggaggaga taatatgctc ctcatgataa acgatgtgat
     5461 tttcaaataa ataatgctgg gtataatatt ttcttttg
//
LOCUS       DAZGQL010000186         5468 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.187, whole genome shotgun sequence.
ACCESSION   DAZGQL010000186 DAZGQL010000000
VERSION     DAZGQL010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5468
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.187"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            1..145
                     /locus_tag="ACHKIR_004943"
                     /pseudo
     CDS             1..145
                     /locus_tag="ACHKIR_004943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312403.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            498..1064
                     /gene="slp"
                     /locus_tag="ACHKIR_004944"
     CDS             498..1064
                     /gene="slp"
                     /locus_tag="ACHKIR_004944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HFO9992561.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
     gene            1217..1747
                     /gene="dctR"
                     /locus_tag="ACHKIR_004945"
     CDS             1217..1747
                     /gene="dctR"
                     /locus_tag="ACHKIR_004945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFO9992562.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQHAFVI
                     IDTLMNNVFHSNLLTQIERLKPVHVIIFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEADVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(1808..2836)
                     /gene="chuS"
                     /locus_tag="ACHKIR_004946"
     CDS             complement(1808..2836)
                     /gene="chuS"
                     /locus_tag="ACHKIR_004946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hematinate-forming heme oxygenase ChuS"
                     /protein_id="HFO9992563.1"
                     /translation="MNHYTRWLELKEQNPGKYARDIAGLMNISEAELAFARVTHDAWR
                     MRGDIREILAALESVGETKCICRNEYAVHEQVGAFTNQHLNGHAGLILNPRALDLRLF
                     LNQWASVFHIKENTTRGERQSIQFFDHQGDALLKVYATDNTDMAAWSELLARFIADEN
                     MPLELKAVDAPVVQTRADASVVEQEWRAMTDVHQFFTLLKRHNLTRQQAFNLVADDLA
                     CKVSNSALAQILESAQQDGNEIMVFVGNRGCVQIFTGVVEKVVPMKGWLNIFNPTFTL
                     HLLEESIAEAWVTRKPTSDGYVTSLELFAHDGTQIAQLYGQRTEGEQEQAQWRKQIAS
                     LIPEVVTA"
     gene            complement(2885..4867)
                     /gene="chuA"
                     /locus_tag="ACHKIR_004947"
                     /gene_synonym="shuA"
     CDS             complement(2885..4867)
                     /gene="chuA"
                     /locus_tag="ACHKIR_004947"
                     /gene_synonym="shuA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent heme/hemoglobin receptor
                     ChuA/ShuA"
                     /protein_id="HFO9992564.1"
                     /translation="MSRPQFTSLRLSLLALAVSATLPTFAFATETMTVTATGNARSSF
                     EAPMMVSVIDTSAPENQTATSATDLLRHVPGITLDGTGRTNGQDVNMRGYDHRGVLVL
                     VDGVRQGTDTGHLNGTFLDPALIKRVEIVRGPSALLYGSGALGGVISYDTVDAKDLLQ
                     EGQSSGFRVFGTGGTGDHSLGLGASAFGRTENLDGIVAWSSRDRGDLRQSNGETAPND
                     ESINNMLAKGTWQIDSAQSLSGLVRYYNNDAREPKNPQTVEASDSSNPMVDRSTIQRD
                     AQLSYKLAPQGNDWLNADAKIYWSEVRINAQNTGSSGEYREQITKGARLENRSTLFAD
                     SFASHLLTYGGEYYRQEQHPGGATTGFPQAKIDFSSGWLQDEITLRDLPITLLGGTRY
                     DSYRGSSDGYKDVDADKWSSRAGMTINPTNWLMLFGSYAQAFRAPTMGEMYNDSKHFS
                     IGRFYTNYWVPNPNLRPETNETQEYGFGLRFDDLMLSNDALEFKASYFDTKAKDYIST
                     TVDFAAATTMSYNVPNAKIWGWDVMTKYTTDLFSLDVAYNRTRGKDTDTGEYISSINP
                     DTVTSTLNIPIAHSGFSVGWVGTFADRSTHISSSYSKQPGYGVNDFYVSYQGQQALKG
                     MTTTLVLGNAFDKEYWSPQGIPQDGRNGKIFVSYQW"
     gene            complement(5279..>5468)
                     /locus_tag="ACHKIR_004948"
     CDS             complement(5279..>5468)
                     /locus_tag="ACHKIR_004948"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992565.1"
                     /translation="TALQAYLFLIFIKMLRQLTFLLPTKFQRGKKLRTQRYRGLQSKE
                     KRKLSLLTTKFSDEVMGE"
BASE COUNT         1512 a         1304 c         1185 g         1467 t
ORIGIN      
        1 caatgttata agcaagttaa agcctttacg acactctccc gatacaataa aattcaaaca
       61 tgggcagaaa tgtctgaata tcaattcgaa gtatttcaat acgagacgct taacccaaaa
      121 aaaatgtcat acgcacctta tttaaagaga tcgctacctg atgaaaaaac tataaattat
      181 ggggtggaaa tagaaacacc aactggcaaa agaattcgct tattaaatca ttaccagaat
      241 ataatcccat aatattgaac attagcactt tataatcatt gaataaaaat agattttatg
      301 tacctttaaa aaaaggctat tttatggggc gatggatact catgtcacgc cccaaaatta
      361 acagaattaa actaaacaga agggatataa acatcagaca ggtttacgtt actatcaggc
      421 atatcgcctc agaatcagat gaaaactata aagaaatatc tattatggtt ttaatatttg
      481 ttgataagga tagtaacatg aacatgacaa aaggtgcact catcctcagc ctttcatttt
      541 tgcttgccgc atgtagttca attccgcaaa atatcaaagg caataaccaa cctgatattc
      601 aaaaaagttt tgttgctgtt cataaccagc cggggttata tgttggtcaa caagcgcgct
      661 ttggcgggaa ggttatcaac gttatcaatg gcaaaacgga tacgttgtta gaaatcgctg
      721 tattaccgtt ggatagctat gcgaagcctg atattgaagc caactatcag ggccgactgc
      781 tcgccagaca aagcggcttc cttgatccag tgaactatcg taatcacttt gttaccatcc
      841 tcggtaccat tcagggtgaa caacctggct ttatcaataa agtcccgtat aacttcctgg
      901 aagtgaatat gcagggcatc caggtgtggc atttgagaga agtggttaat accacctata
      961 acctgtggga ttacggctat ggtgcattct ggccggaacc gggctggggc gcgccttact
     1021 acaccaatgc ggtgagtcaa gtaacacctg agctggtcaa ataacaccac cgagagatgc
     1081 agatgctttc tcagcatctg catcatgcat tacatcaaat taatacacag ccagctaact
     1141 attattataa gccctgtcct gttaattacc tttggcaaac tgattataaa gttaatgtcc
     1201 gcaccaggag tcggttatgt ttcttataat taccagggat acgatgttct tcactgcgat
     1261 gaaaaacatt ctgagtaaag gtaatgtcgt tcatatacag aacgaagaag agatcgacgt
     1321 aatgttgcat cagcatgcct tcgtcattat tgatacatta atgaataatg tatttcattc
     1381 taatttgctc actcagattg aacgattaaa acctgtccat gtgattattt tctctccctt
     1441 taatattaaa cgctgcctgg ggaaagtacc ggtgaccttt gttccgcgaa ctatcaccat
     1501 catcgacttt gtcgcactca tcaatggcag ttactgctct gtgcctgaag cggatgtgtc
     1561 cctttcgcgc aagcagcatc aggttctgag ctgcattgcg aatcaaatga caacggaaga
     1621 tattctggag aaactgaaaa tatcgctaaa aacgttctac tgccataaac acaatatcat
     1681 gatgatcctc aatcttaagc ggatcaatga gttggtacgc catcagcata ttgattatct
     1741 ggtgtgaacg attgatcaat ataaagaggc ccagcaataa atagccagac ctcccgttaa
     1801 ttatacgtta tgcagtaacg acttccggta tcagcgaagc aatttgctta cgccattgcg
     1861 cttgctcctg ttcgccttct gtacgttgac cataaagttg cgctatctgc gtaccatcat
     1921 gggcaaacaa ttccagactg gttacgtagc catcgctggt cggtttacgg gtaacccagg
     1981 cttcagcaat gctctcttct aatagatgaa gagtaaacgt cgggttgaaa atattcagcc
     2041 aacctttcat tggcaccact ttttctacca caccagtgaa aatctgtacg cagccacggt
     2101 tgccaacaaa caccatgatt tcattgccat cctgctgtgc agattcaaga atttgcgcca
     2161 acgcactgtt ggatactttg caggccaaat catctgccac cagattgaac gcctgttggc
     2221 gcgtcaggtt gtggcgcttg agcaacgtaa aaaactgatg aacgtcggtc atcgcccgcc
     2281 actcttgctc gaccacactg gcatcggctc gcgtttgaac aactggcgca tcaacggctt
     2341 ttaactcaag cggcatattc tcatcggcga taaaccgtgc cagaagctca ctccatgccg
     2401 ccatatcggt attgtcggtg gcataaactt ttagtaatgc atcgccctga tgatcaaaga
     2461 actgaatact ctggcgttcg ccacgagtcg tattttcttt aatgtgaaaa acactggccc
     2521 attgattgag aaacagacgc aaatccagcg cgcgtggatt gaggatcaat ccggcatgtc
     2581 cgttcaaatg ctggtttgtg aacgcaccaa cttgctcatg gactgcatat tcattacgac
     2641 aaatacattt ggtctcgcca acactttcga gcgccgccag aatttcacgg atatcgccgc
     2701 gcatccgcca cgcatcgtgc gtgacgcgcg caaatgccag ttctgcttcg ctaatattca
     2761 ttaaacctgc gatgtcacgc gcgtactttc cgggattttg ttcttttaac tcaagccagc
     2821 gtgtgtagtg gttcattgtc tctcttcctt ccagataaat gccccgaaat atcggggcag
     2881 atgattacca ttgataactc acgaaaattt ttccattacg accatcctgt gggatgcctt
     2941 gtggcgacca gtactctttg tcgaaagcgt tacccaacac caaagtagtg gtcatacctt
     3001 tgagcgcctg ttgtccttga taactgacgt agaaatcatt cacgccatag cctggttgtt
     3061 tgctgtaact gctgctgata tgtgttgagc gatcggcaaa cgtaccaacc cacccaacag
     3121 agaagccact gtgagcgatc ggaatattca gggtgctggt aacggtatcc gggttaatac
     3181 tggagatata ttcgccggta tcggtgtctt tgccgcgggt acggttatag gccacatcaa
     3241 ggctaaacag atcagtggta tatttcgtca tcacatccca accccagatt ttggcgttcg
     3301 ggacgttata cgacatagtt gtcgccgccg cgaaatcgac ggtcgtggag atgtaatcct
     3361 tcgctttggt atcaaagtag ctggctttaa attccagagc atcattggac aacatcaggt
     3421 catcaaaacg cagcccaaaa ccgtactcct gagtttcgtt agtttccgga cgtaagttcg
     3481 ggtttggcac ccaatagttg gtatagaagc gaccaatcga gaagtgctta gaatcgttat
     3541 acatttcgcc catcgtcggg gcgcggaatg cctgagcata tgagccaaat aacatcagcc
     3601 agttagtcgg gttgatagtc atccccgcgc gagatgacca tttgtcggca tcaacatctt
     3661 tgtagccgtc gctgctaccg cgataactgt catagcgggt tccgccaagc agggtaatcg
     3721 gcagatcgcg taaggtgatc tcatcttgta gccagccaga gctaaaatcg atttttgctt
     3781 gcgggaagcc cgtcgtcgcg ccacccggat gttgttcctg acgataatac tcaccgccat
     3841 atgtcagtaa gtgagaagcg aaactgtcgg caaatagagt ggaacggttc tccagccttg
     3901 ctccttttgt tatctgttca cgatactcgc cggaactccc cgtgttttgc gcattaatac
     3961 ggacttccga ccagtaaatt tttgcatctg catttaacca gtcgttaccc tgcggggcga
     4021 gtttataaga aagctgcgca tcgcgttgaa ttgttgaacg atcgaccatc gggttgctgc
     4081 tatcagaagc ttcaacggtc tgcggatttt ttggttcacg cgcgtcgttg ttgtagtaac
     4141 gcactaaacc gctcagagac tgagctgaat caatttgcca ggtccctttc gccagcatgt
     4201 tattaatgga ttcgtcattc ggcgcggttt caccattgct ctggcgtaaa tcaccccgat
     4261 cgcgactgga ccaggccaca ataccatcca gattttcagt tcgcccaaac gcgctcgcgc
     4321 ctaatcccag gctatggtcc cccgtgccgc cagtaccaaa gacacgaaaa ccactgcttt
     4381 gtccttcctg caataaatct tttgcatcga ccgtatcgta ggagatcact ccacccagcg
     4441 cgccactgcc atacagtaat gctgaaggtc cgcgaacaat ctcaacacgc ttgatcagcg
     4501 ccggatcgag aaaagtgcca ttcaggtgtc cggtatccgt tccctggcga acaccatcga
     4561 caagaaccag cacgccgcga tgatcatagc cacgcatatt tacatcctga ccgttggttc
     4621 gtccggtacc atcaagagta attccaggaa catgacgcag cagatcggtg gctgaagtag
     4681 ccgtttgatt ttcaggagcg gaagtgtcga tgacgctgac catcataggc gcttcgaagg
     4741 aactacgggc attccccgtt gccgtaacgg tcatggtttc agtagcaaaa gcaaacgttg
     4801 gcaaggtggc agaaacagcc aaagccaaca aactcaaacg caacgaggta aattgcggac
     4861 gtgacatagc gattctccat gaggaaaata aaaaatgctg gctgcctggg ttaataccgg
     4921 ggtggctggc gaaaaataaa tttatttaaa attcagagcg ataatattcg ccgggtgagt
     4981 ttacaggcgt ttatatccca tgaaattgcc tggtatctcg gcaaatacgc aaaagcacgc
     5041 cctctttgtc aggcaaaccg atatgcccca tcaggagtga agagcgctgt gcgttacccc
     5101 ttgtaaatga tatggctttg ttatccatat ggccgtattt tgttaaatgc aaacgtgata
     5161 atgagaatta ttatcgatca attttttatg atcaagcttt tttttaaccg ccattaacac
     5221 aataaacagg gggttatgaa acttatcctg cgccctggaa accactttgt ttttatggtt
     5281 attcccccat gacctcatca ctaaactttg tcgttaacag tgataatttt ctcttctctt
     5341 tgctctgcaa gccgcgatat ctctgggttc tcagcttttt cccgcgctga aatttagtcg
     5401 gtaacaagaa cgttagctga cgaagcatct tgataaaaat caaaaataaa tacgcttgta
     5461 acgccgtg
//
LOCUS       DAZGQL010000187         5447 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.188, whole genome shotgun sequence.
ACCESSION   DAZGQL010000187 DAZGQL010000000
VERSION     DAZGQL010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5447
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.188"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            45..1379
                     /gene="garP"
                     /locus_tag="ACHKIR_004949"
     CDS             45..1379
                     /gene="garP"
                     /locus_tag="ACHKIR_004949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HFO9992566.1"
                     /translation="MMLDTVDEKKKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVIGFVLTALWIKLIHNPTDHPRMSAEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            1395..2165
                     /gene="garL"
                     /locus_tag="ACHKIR_004950"
     CDS             1395..2165
                     /gene="garL"
                     /locus_tag="ACHKIR_004950"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008672 - 2-dehydro-3-deoxyglucarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HFO9992567.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            2195..3085
                     /gene="garR"
                     /locus_tag="ACHKIR_004951"
     CDS             2195..3085
                     /gene="garR"
                     /locus_tag="ACHKIR_004951"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFO9992568.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            3182..4327
                     /gene="garK"
                     /locus_tag="ACHKIR_004952"
     CDS             3182..4327
                     /gene="garK"
                     /locus_tag="ACHKIR_004952"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HFO9992569.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADG
                     GEGTVEAMIAATQGAERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDSGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTNDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            4360..4736
                     /gene="rnpB"
                     /locus_tag="ACHKIR_004953"
     ncRNA           4360..4736
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHKIR_004953"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(4945..5046)
                     /locus_tag="ACHKIR_004954"
                     /pseudo
     CDS             complement(4945..5046)
                     /locus_tag="ACHKIR_004954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5302..>5447)
                     /gene="tdcR"
                     /locus_tag="ACHKIR_004955"
     CDS             complement(5302..>5447)
                     /gene="tdcR"
                     /locus_tag="ACHKIR_004955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional activator TdcR"
                     /protein_id="HFO9992570.1"
                     /translation="RYLLENKHLFYQYFKISNTGIDHLEQLINVNFFSSDRTSFCECN
                     RFP"
BASE COUNT         1307 a         1268 c         1514 g         1358 t
ORIGIN      
        1 ccccataata ataaaaatca gcataagtac ccgaggtaaa taaaatgatg ctggacaccg
       61 ttgacgaaaa aaagaaaggc gtgcataccc gctatttaat attactgatt atttttattg
      121 ttaccgccgt taactacgcc gatcgtgcaa cgctgtctat tgctggtacc gaagtggcaa
      181 aagagttgca gttaagtgcg gtttcgatgg gttacatctt ctctgctttt ggctgggcct
      241 acttgctgat gcaaatcccc ggcggctggc tgcttgataa gtttggttcg aaaaaagttt
      301 acacctacag cctctttttc tggtcgctat tcaccttcct gcaaggcttt gttgatatgt
      361 tcccgctggc ctgggcaggg atctccatgt tctttatgcg ctttatgctg gggttctcgg
      421 aagcgccatc attcccggcg aacgcccgta ttgtcgccgc ctggttcccg acgaaagaac
      481 gtggtactgc ctccgccatc tttaactcgg cgcaatattt ctcgctggcg ctcttttcgc
      541 cgctgcttgg ctggctgact ttcgcgtggg gctgggagca cgtctttacc gttatggggg
      601 taattggttt tgtgctgacg gcgctgtgga tcaagttgat tcataacccg acagatcacc
      661 cacgtatgtc tgcggaagag ctgaagttta tctcggaaaa tggcgcggtg gtcgatatgg
      721 accacaaaaa gccgggcagt gcggcagcaa gcggacccaa actgcattac atcaagcaat
      781 tgctctctaa ccgcatgatg ctgggcgtat ttttcggaca atattttatc aacaccatca
      841 cctggttctt cctcacctgg ttcccaattt atctggtgca ggaaaaaggc atgtcgattc
      901 tgaaagtggg tctggtcgcc tcgattccgg cactgtgtgg ctttgcgggc ggcgtgctgg
      961 gaggtgtctt ctcggattat ctgatcaaac gcggtttgtc cctgaccctg gcacgtaagc
     1021 taccaattgt gctgggaatg ttgctggctt ccaccatcat cttatgtaac tacaccaaca
     1081 acaccacgct ggtggtcatg ctgatggcgc tggctttctt tggtaaagga tttggtgcgc
     1141 tgggctggcc agtgatttct gacaccgcgc cgaaagagat tgttggcctc tgcggcggcg
     1201 tctttaacgt ctttggcaat gttgcctcca ttgtcactcc actggtgatt ggctacctgg
     1261 taagtgaact gcactccttc aatgcagcac tggtttttgt gggatgttca gcgctgatgg
     1321 cgatggtctg ctacctcttc gtagtcggcg acattaaacg tatggaattg cagaaataag
     1381 caaaggtata agcgatgaat aacgatgttt tcccgaataa attcaaagcc gcactggctg
     1441 cgaaacaggt acaaattggt tgctggtcag ctctctctaa cccgattagc actgaagttc
     1501 ttggtttggc tgggtttgac tggctggtgc tggatggtga acatgcgcca aacgatattt
     1561 ccacgtttat tccgcagtta atggccctga aaggcagcgc cagcgcgcca gtagtgcgag
     1621 tgccgaccaa cgagccggta attattaagc gtcttctgga tatcggtttc tataacttcc
     1681 tgattccttt tgtagaaaca aaagaggaag cagagcaggc ggtggcatca actcgttacc
     1741 caccggaagg cattcgcggc gtctccgttt ctcaccgcgc caatatgttt ggcaccgtgg
     1801 cggattattt cgctcagtcg aacaagaaca tcactattct ggtccagata gaaagtcagc
     1861 agggcgtaga taacgtcgat gctattgccg ctaccgaagg tgtagacggc atcttcgtcg
     1921 gccccagcga tctggctgcg gcattaggcc atctcggcaa tgcctcgcac ccggatgtac
     1981 aaaaagcaat tcagcacatt tttaaccgtg ccagcgcgca cggcaaaccc agcggcatcc
     2041 tcgcaccagt cgaggctgat gcgcgtcgtt atctggaatg gggcgcgacg tttgtggccg
     2101 tcggcagcga tctcggcgtc ttccgctctg ccactcagaa actggctgat acctttaaaa
     2161 aataaccacc accgcaataa gagagatgat tgatatgact atgaaagttg gttttattgg
     2221 ccttgggatt atgggtaaac caatgagtaa aaaccttttg aaagcaggtt actcgctggt
     2281 ggttgctgac cgtaacccag aagctatcgc tgacgtgatt gctgcaggtg cagaaacagc
     2341 ctctacggct aaagcgatcg ctgaacagtg cgacgtaatc ataaccatgc tgccaaactc
     2401 ccctcatgtg aaagaggtgg cgctgggtga gaatggcatt attgaaggcg cgaagccagg
     2461 tacggtattg atcgatatga gttctatcgc accgctggca agccgtgaaa tcagcgaagc
     2521 gctgaaagcg aaaggcattg atatgctgga tgctccggtg agcggcggtg aaccgaaagc
     2581 catcgacggt acgctgtcag tgatggtggg cggcgacaag gctattttcg acaaatacta
     2641 tgatttgatg aaagcgatgg cgggttccgt ggtgcatacc ggggaaatcg gtgcaggtaa
     2701 cgtcaccaaa ctggcaaatc aggtcattgt ggcgctgaat attgctgcga tgtcagaagc
     2761 gttaacgttg gcaaccaaag cgggcgttaa cccggatctg gtgtatcagg caattcgcgg
     2821 tgggctggcg ggcagtaccg tgctggatgc caaagcgccg atggtgatgg accgcaactt
     2881 caaaccgggc ttccgtattg atctgcatat taaggatctg gcgaatgcgc tggatacttc
     2941 tcacggcgtc ggcgcgcaac tgccgctcac agctgcggtt atggagatga tgcaggcact
     3001 gcgtgcagat ggtttaggaa cggcggatca tagcgccctg gcgtgctact acgaaaaact
     3061 ggcgaaagtc gaagttactc gctaatgacg tcgtgcccgg atggcgtatt gcaatccggg
     3121 cctggaatcc aggccgaata agagaaacgc cctccggcgt catgtggtaa caggcatagg
     3181 tatgaaaatc gtaatcgccc cagactctta taaagaaagt ttatctgcca gcgaggttgc
     3241 gcaggcgata gaaaaaggat ttcgggaaat ttttcctgat gcacagtatg tttctgttcc
     3301 ggttgccgac ggtggtgaag gaacggtgga agcgatgatt gcagccaccc agggggctga
     3361 acgtcacgcc tgggttacag ggccgctggg cgagaaagtg aatgccagtt gggggatctc
     3421 cggcgatggc aaaaccgcgt ttatcgaaat ggcggcggcc agtgggctgg aactggtacc
     3481 tgcggaaaaa cgcgatccac tcgtgaccac ttcacgcggt actggtgagt taatcctgca
     3541 ggcgctggag agcggtgcga caaacattat tatcggcatt ggcggcagcg ccaccaacga
     3601 cggcggcgca ggcatggtac aggcgctggg ggcgaaatta tgcgatgcca acggcaatga
     3661 aattggtttt ggcggcggta gtcttaatac tctgaatgat attgatattt ccggtctcga
     3721 tccgcgctta aaagattgtg tcatccgcgt cgcttgtgat gtcaccaacc cgttagtcgg
     3781 cgacagtggt gcttcgcgca tctttggccc acaaaaggga gccagtgagg cgatgattgt
     3841 cgagctggac aataacctct ctcactatgc cgatgttatt aaaaaagcgc tgcatgttga
     3901 tgtgaaagat gtccccggcg caggagctgc gggcggtatg ggcgcggcgc tgatggcgtt
     3961 tcttggtgca gaactgaaaa gtggtattga aatcgtcact acggcgctga atctggagga
     4021 acatattcac gattgtacgc tggtgatcac tggtgaaggg cgtattgata gccagagtat
     4081 tcacggcaag gtaccgattg gtgtcgcaaa cgtggcgaag aagtaccata aaccggtgat
     4141 tggcattgcg ggtagcctga ccaatgatgt tggcgttgta catcagcatg gcattgatgc
     4201 ggtcttcagc gtattgacca gcattggtac gttggacgaa gcattccgcg gggcttatga
     4261 caatatctgc cgtgcttcac gtaatatcgc cgcgacactg gcgattggaa tgcgcaacgc
     4321 ggggtgacaa gggcgcgcaa accctctata ctgcgcgccg aagctgacca gacagtcgcc
     4381 gcttcgtcgt cgtcctcttc gggggagacg ggcggagggg aggaaagtcc gggctccata
     4441 gggcagggtg ccaggtaacg cctggggggg aaacccacga ccagtgcaac agagagcaaa
     4501 ccgccgatgg cccgcgtaag cgggatcagg taagggtgaa agggtgcggt aagagcgcac
     4561 cgcgcggctg gtaacagtcc gtggcacggt aaactccacc cggagcaagg ccaaataggg
     4621 gttcataagg tacggcccgt actgaacccg ggtaggctgc ttgagccagt gagcgattgc
     4681 tggcctagat gaatgactgt ccacgacaga acccggctta tcggtcagtt tcacctgatt
     4741 tacgtaaaaa cccgcttcgg cgggtttttg cttttggtgc ccagggtaag atgaatatct
     4801 acggtattaa taatagactt tgtaagaagt tattaagtct atattgcatt taaaaggtcg
     4861 taattataca tatatttctc tcattaattg taagttcgca ggtattgtcc gatcgttgaa
     4921 tcatttggac gttataacct aaatttttac cgtccaataa tcaatatttc tgttgtgatt
     4981 gttaaccact gcgtgcattg gcgggattga agggtgaaaa atatgtgcaa ttgggaagcc
     5041 tttcattgat gcctgccttg tcatcatgac aaacaataat agatgcttat tttcctcgca
     5101 gggttctttc gcactatcaa aaaatgctca tttacccggc attacagtca acgaagcaac
     5161 attctatggc ctacgctgat gtaaagcaca gtgagaatcc cccttgttgt cttcgtctga
     5221 ccttatcgcg gcgaccagca attattcgtt ttgtgatcct caacacaaaa tgctattcgt
     5281 cgcctatgag gggagaggaa tttatggaaa acgattgcat tcacaaaatg acgtcctgtc
     5341 agaagagaaa aagttgacgt taatcaattg ttcgagatga tcgatacctg tattcgaaat
     5401 tttaaaatat tgataaaata aatgtttgtt ctccaacaaa tagcgtg
//
LOCUS       DAZGQL010000188         5439 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.190, whole genome shotgun sequence.
ACCESSION   DAZGQL010000188 DAZGQL010000000
VERSION     DAZGQL010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5439
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.190"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..266)
                     /locus_tag="ACHKIR_004956"
     CDS             complement(<1..266)
                     /locus_tag="ACHKIR_004956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFO9992571.1"
                     /translation="MNIRKLFCPDNTPRILLFLFFFVISVITTIACGYTEKNATGNVL
                     LLFLLLLLAHRNTLTSITALLFLFCCALYAPAGMTYGKINNSFIV"
     gene            complement(418..1158)
                     /locus_tag="ACHKIR_004957"
     CDS             complement(418..1158)
                     /locus_tag="ACHKIR_004957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HFO9992572.1"
                     /translation="MNKVIAVSALAMAGMFSTQALADESKTGFYVTGKAGASVMSLAD
                     QRFLSGDGEETSKYKGGDGHDTVFSGGIAAGYDFYPQFSIPVRTELEFYARGKADSKY
                     NVDKDSWSGGYWRDDLKNEVSVNTLMLNAYYDFRNDSAFTPWVSAGIGYARIHQKTTG
                     ISTWDYGYGSSGRESLSRSGSADNFAWSLGAGVRYDVTPDIALDLSYRYLDAGDSSVS
                     YKDEWGDKYKSEVDVKSHDIMLGVTYNF"
     gene            1382..1567
                     /locus_tag="ACHKIR_004958"
                     /pseudo
     CDS             1382..1567
                     /locus_tag="ACHKIR_004958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708738.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            1796..4105
                     /locus_tag="ACHKIR_004959"
     CDS             1796..4105
                     /locus_tag="ACHKIR_004959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB N-terminal domain-containing protein"
                     /protein_id="HFO9992573.1"
                     /translation="MELWIFIVAIYLIYILFFKKKKTKSAGSYTSAPVKAPPKEWLAN
                     NKKNQSVMQNEDSEDDNLATFTLSGGRNVEYRITATQRQNFSKTTGALARWIFPGEMI
                     TVSGIEVTGGHFYLGQRMKPAGHNSGGYYDGNNEASLVDDTLKIYPTPYIYEDSSLGY
                     WPSFSSLSPEARGAYLSWLASDRCDTSCPIGYVFIYLYGLERRALVDSRDNNISDDEF
                     YSLFKEICRLRSVFNENRSFRNYSSQLLEAMSILRPNENLAAALNDDSEFSNSMQFRL
                     ILAKTVDTGAPVPADLALTWVTNHAEYSLRTPARRCAKEFAALFKRRYTLKYGEGMVV
                     KPNKARLRLDYTPASPSLRGIRLPVPDLPDPGALKGPVQKLMAIADICTGELDAYSRY
                     LGRKGTSANDTAAILLLPSEIVNESAEKILSTFKHWADDAIRSKGGIVSVADYWSHMN
                     ATCPAKINKKEADLMQAFAQKMGYCLAPDPYHHHVKADVDGVLVLFPAGERGRFSPYP
                     EFITAVLTLRLGSVVALIDNSLDQAEQKVLENAINNNASFSDDEKRSLHAYLTWQLHT
                     PANMTGMKSRIELLGAAEKSAVGKVIVSVACSDGRITPAEIKQLEKIYTSLGLDPSSV
                     SSNIHQHSATETALVSSVPANQSTAGFTLDANVLARHESATDDVRKLLNSIFTEEEPE
                     QPESAPASLTGEGGLDSAHSQLYRRLLEKEQWSRKEATELCSNWNLMLGGALEVINDW
                     SYAVVDAPVLEDADDHIWIDLEIAKELEG"
     gene            4109..4510
                     /locus_tag="ACHKIR_004960"
                     /pseudo
     CDS             4109..4510
                     /locus_tag="ACHKIR_004960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000117530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system ATP-binding domain-containing
                     protein"
     gene            complement(4481..4689)
                     /locus_tag="ACHKIR_004961"
                     /pseudo
     CDS             complement(4481..4689)
                     /locus_tag="ACHKIR_004961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4724..4858)
                     /locus_tag="ACHKIR_004962"
     CDS             complement(4724..4858)
                     /locus_tag="ACHKIR_004962"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992574.1"
                     /translation="MSEIKIIGIELAKTNFYPFNINDYGKTVGKIKFSRSNLLNLLVQ
                     "
     gene            complement(5082..>5439)
                     /locus_tag="ACHKIR_004963"
     CDS             complement(5082..>5439)
                     /locus_tag="ACHKIR_004963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HFO9992575.1"
                     /translation="ELDECMPKKSMFCIDPPYFDKGSLLYTNFYNAEDHKKVSEAILK
                     LKHPWLLTYDNALEINELYNNCARYTYPLNYSAATKRVGTELLITQDGIHIPNNLGIT
                     RVINKEKVMSTDEVSI"
BASE COUNT         1569 a         1192 c         1187 g         1491 t
ORIGIN      
        1 acaataaaac tgttgttgat tttaccgtac gtcatacccg caggcgcata cagtgcacaa
       61 cagaacagaa ataacagagc tgtaatggat gtaagggtat ttctgtgtgc aaggagcaga
      121 agaaggaaca gcagcaacac attcccggtg gcattcttct cagtgtatcc gcatgcaatt
      181 gtggttatga cagaaataac aaaaaagaat aaaaacaata aaatccgtgg tgtgttgtcc
      241 ggacaaaaca gttttctgat attcatcgga gtatatcgac aacattattg tgaagagaat
      301 aaaaatcaga agtctcctgt gaaacagata acaaacaacc ctgcagtata attactgcag
      361 ggagtcttcg tttaattaca gaagatgggt aattattatc tttcaggagc agttttgtca
      421 gaaattataa gtcacaccaa gcatgatgtc atgactttta acatcaactt ctgatttata
      481 tttatcgccc cactcgtcct tgtaactcac actgctgtca cctgcatcaa gatagcgata
      541 gctgaggtcc agagcgatat ccggggttac gtcatagcga acacccgcgc caaggctcca
      601 tgcgaagttg tcagcagagc ctgaacgtga caacgattcg cgaccactgc ttccgtaccc
      661 ataatcccag gtactgatac cggttgtttt ctggtgaatt ctggcgtagc caatccctgc
      721 agatacccat ggtgtgaatg cactgtcatt ccggaagtca tagtacgcat tcagcatcag
      781 tgtgttgact gacacctcat tcttcaggtc atcacgccag taaccgcctg accagctgtc
      841 tttatctacg ttatacttcg aatcagcttt tccacgagcg taaaactcca gttccgtacg
      901 aaccggaata ctgaactgcg ggtaaaaatc ataaccggcc gcgataccgc cactgaatac
      961 cgtatcatgg ccatcaccgc ctttatattt tgatgtttcc tctccatcac ccgacaggaa
     1021 acgctggtct gcaagtgaca taacagaagc acctgcttta ccggtcacat aaaagcctgt
     1081 tttgctctca tcagccagag cctgggtcga aaacatgcct gccatggcaa gcgctgaaac
     1141 cgcaataacc ttattcattt caatcatctc cgctatcact ttccccgaac gataaatggg
     1201 gttgctcatt ctaaaaccat gcaaaagaca gggtaaaagg aatgttttcg atcgaatcga
     1261 tcgataaaat cgatcgtgcc cgataaagtt ttcttatcgt ccccataaaa atggtaaggg
     1321 ctggtttaag aggtaatcgc agcggcatgg aagtattaag accaaaacag cacgagataa
     1381 agcggctgaa cgtcagatga gccacatgga acgtaattca ttaagggcgg cttatatcca
     1441 taaaacagaa cacctggaat agcgtcggtt gatgctacag tggtgggggg attttctgga
     1501 tacaaactcg aaagaaaata ccagcccctt tgagtatgcg aagattaaca atccattaaa
     1561 atattaagca ctccgggcaa tctgcccgga gtgctttagc tggcataacg ggctactgat
     1621 ctcctctggt tacagataac gacttcagcc ctctgaaaga gaaaagcgga agctcaggaa
     1681 ggttgtgatt tgcttttaac ctttttaatt tagaagtatt attttgagat taatccactt
     1741 cccatcgtca tcctttttgg tgtgcgtgaa aagctatttt taaggttact gcctcatgga
     1801 gttatggata tttatagtag ctatttattt gatttatatt ctttttttca aaaagaagaa
     1861 aactaagtcc gcgggctctt atacttccgc tcctgtgaaa gcaccaccta aagaatggct
     1921 tgctaacaac aaaaaaaatc agtctgtaat gcaaaacgag gacagcgaag atgacaatct
     1981 ggctacgttt acactaagtg gcgggcggaa tgtcgaatac aggataacag ccactcaacg
     2041 ccagaatttc tcaaaaacta ctggtgcttt ggctcgttgg atttttccgg gagagatgat
     2101 cacagtcagt gggatagagg ttaccggcgg tcacttctat cttgggcagc gtatgaaacc
     2161 tgccgggcat aactcaggcg gttattatga tggcaataac gaggcatcgc tagttgacga
     2221 cacgcttaaa atatacccca caccttacat ttacgaagat agttcattag ggtattggcc
     2281 cagtttttcc tcactttcac ctgaagctcg tggtgcgtac ctgagttggc ttgccagcga
     2341 cagatgcgac acttcctgcc caattggtta cgttttcatt tatctgtatg gtctggaaag
     2401 aagggcgctt gttgacagca gggacaacaa tatatccgac gatgagttct attctctttt
     2461 caaagagatc tgccgtctca gatcggtttt caatgaaaac cgatctttcc ggaattactc
     2521 atcccagtta cttgaagcga tgtccattct tcgtccgaac gaaaatcttg cagcagctct
     2581 caacgatgat tcggagttca gcaatagcat gcagttcagg ttaatcctgg caaagactgt
     2641 tgatacagga gccccggttc cagcggatct ggctctgacg tgggtgacaa accatgctga
     2701 atactcatta cgaacccccg cccgccgctg tgcgaaagaa tttgctgcac tatttaaacg
     2761 gcgttacacc cttaaatacg gtgaagggat ggtagtgaag ccgaataaag cgcgtctgcg
     2821 tttagattac acgcctgcaa gtcccagttt gcgaggtatt cggcttcctg ttcccgactt
     2881 gcctgatcca ggtgcattga aaggcccagt tcagaagctt atggccattg ccgatatatg
     2941 cacaggtgaa ctggatgctt acagtcgtta ccttggtaga aaaggtactt cagcaaatga
     3001 taccgctgca attttgttgc tgccttcaga aatcgttaac gaaagcgcag aaaaaatatt
     3061 aagtacattc aaacactggg ctgatgacgc aatccgcagt aagggtggga tagtttcagt
     3121 tgctgactac tggtcacata tgaatgccac ctgtccggcg aagatcaata aaaaagaggc
     3181 cgacttaatg caagcctttg cacagaaaat gggatactgt ctggctcctg acccctatca
     3241 ccaccacgta aaggcagatg tcgatggtgt acttgtctta ttccctgctg gtgaaagagg
     3301 acgtttctca ccttatcctg aatttatcac agctgtattg acgctccgat taggttcggt
     3361 agtcgccttg attgacaatt ctctcgatca agccgaacag aaagtgcttg agaatgccat
     3421 caacaacaat gccagcttca gcgatgatga aaagcgctct ctgcacgcat atttaacgtg
     3481 gcagcttcat actccggcga atatgacggg aatgaaaagc cggattgaat tactcggtgc
     3541 agcggaaaaa tcagctgtgg gtaaggttat cgttagtgtc gcttgctctg acggcagaat
     3601 tacgcctgcc gaaatcaaac agcttgagaa gatttataca agcctgggac tggatccctc
     3661 atccgtatca agcaatatcc atcagcattc cgcaactgaa actgctctcg tttcatctgt
     3721 accagctaat cagtcgacgg cagggtttac gctggatgcc aacgtacttg cccgccacga
     3781 atctgccact gatgatgttc gtaaattact gaactcaatt ttcactgaag aagaaccaga
     3841 acaaccagaa agcgctccag cctcattaac cggggaaggg ggacttgact ccgctcatag
     3901 ccaactttat cgcaggttgc tggagaaaga acaatggtcc agaaaagagg cgacagagtt
     3961 gtgtagtaac tggaacctga tgctcggcgg tgcgcttgag gtgattaacg attggtccta
     4021 tgcggtggtt gatgcaccag tgctggaaga tgctgatgat cacatttgga ttgacctgga
     4081 aatagccaaa gaattagagg gttagtgaat gtctgtaaca cgtataagag tgaaagagcg
     4141 cgacgctatc attcagtcac ttaagtcggg agtaacgcca agaataggta ttcagcatat
     4201 ccaggttggc cgcgtgaatg agataaccgc cctccatcag gatattgaac ggattgctga
     4261 tggcggggca agcttcagac ttatcattgg tgagtacggt tccgggaaaa cattcttttt
     4321 aagtgttgta cggtctattg cgttagagaa gaagctggtc acggtcagtg cagacctttc
     4381 tcccgacaga cgtatccact catcgggagg tcaggctcgt aatctctact cagagcttat
     4441 gaaaaatatg tccaccagaa acaaaccaga tggcaatgca ttacttagcg ttgtggaaag
     4501 gtttgttaca gtcaaggcag tcaccaaact cgataataaa gcggctcatt tccatacgtc
     4561 tgtccttaag gggcattgtt catttctgtg accaagccgc accacctttt ttactgattc
     4621 gtctgtcggg aacactttgc gtttcttgat gacataacgg atcacactat tcaacgactc
     4681 tatggcattt tgcacatact tccatagcaa ctgtcatcgg tagctactga accagcaagt
     4741 taagtaagtt actacgtgaa aattttattt ttccaacggt ttttccataa tcattaatat
     4801 taaaaggata aaaatttgtt ttagccagct cgataccaat gatttttatt tcagacatga
     4861 tatccttctt acatttcatt gattaaagtt tatccatcat ggtatgatct gcttctaccg
     4921 tgaaggaggg gctgacactc aatggaccaa aaactcacgt taaacaattg tagcaaagga
     4981 aagttaaagg atcctgcgag gatcctttga taggtgttaa aattgcattc ttattaccat
     5041 ataagatcag ttatctctca gtagctgtga gcatattaaa tttatattga cacttcatcc
     5101 gtactcatta ctttttcctt attgataact cttgtaattc ctaaattatt tggaatatgt
     5161 atgccatctt gagttataag taactctgtt ccaactcgtt tcgttgcagc agaataattc
     5221 aatgggtacg tatagcgtgc acaattatta tataattcat taatttctaa tgcattatca
     5281 taagttaaaa gccatggatg ctttagtttt aaaatagctt cggacacttt tttatgatct
     5341 tcagcattat aaaagttagt gtagagcaat gagcctttat caaagtaagg aggatcaata
     5401 caaaacattg atttttttgg catgcattca tcaagttcg
//
LOCUS       DAZGQL010000189         5431 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.191, whole genome shotgun sequence.
ACCESSION   DAZGQL010000189 DAZGQL010000000
VERSION     DAZGQL010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5431
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.191"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(36..773)
                     /gene="bamD"
                     /locus_tag="ACHKIR_004964"
     CDS             complement(36..773)
                     /gene="bamD"
                     /locus_tag="ACHKIR_004964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HFO9992576.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQLNAQAEKVAKIIAANSSNT"
     gene            908..1888
                     /gene="rluD"
                     /locus_tag="ACHKIR_004965"
     CDS             908..1888
                     /gene="rluD"
                     /locus_tag="ACHKIR_004965"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HFO9992577.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPEDMVELIEVMRADFEEHKDEVDWL"
     gene            1885..2616
                     /gene="yfiH"
                     /locus_tag="ACHKIR_004966"
     CDS             1885..2616
                     /gene="yfiH"
                     /locus_tag="ACHKIR_004966"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphorylase YfiH"
                     /protein_id="HFO9992578.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAAHAGWRGLCSGVLEETVSCFADNPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASTAFIQHGDKYLADIYLLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2746..5319
                     /gene="clpB"
                     /locus_tag="ACHKIR_004967"
     CDS             2746..5319
                     /gene="clpB"
                     /locus_tag="ACHKIR_004967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFO9992579.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         1338 a         1297 c         1476 g         1320 t
ORIGIN      
        1 gctcaagggc tgccgttttg tgtttcaggt ttctgttatg tattgctgct gtttgcggcg
       61 atgattttcg ctactttttc agcttgcgca ttcaactgca tctgacggta tgcattttcc
      121 atcagcggca gcgcatcacg cgtagcctgg gtatccgggt agtcgcgcaa catgccttct
      181 acgcggttaa cgacggcaac ccatgcacca cgttctgtat agtactcggc aacggagtat
      241 tcatatttcg ccagacgatc tttcaggaat accagacgtt tggtggcatc ggtggtgtac
      301 tgactgttcg gatagccgcg caccagtttg gaaaagtcac taaacgcagc tcgtgcatgt
      361 tgaggatcgc gatcgctacg gtcaacgcca aagaaccctt gcagcgcact gtcatccagc
      421 gccatattgg tcaggccacg catgtacatg acataatcga tattcggatg ggtcgggtta
      481 aggcgaataa aacgatcgat ggcagcctgt gctaacggca aatcggcgtt tttatagtag
      541 gcgtagatga gatccagctg cacctgctgc gaatacggac caaacggata gcgattatct
      601 aacgcttcca gttgcgttat tgcctgtctc cagttaccgt cctgcagctt ttgttgtgca
      661 gtcgcgtaaa tttcatttgg cggattatca ggtacttctt cctttgaccc cgagcaaccc
      721 gccaaaaaca ggcttagtgt ggcggctgcc accagatatt tcatgcgcgt catgacgttt
      781 tgactttcct caaaatgtaa tacgggagat tctctgttcc tgctcccggt taagaccagc
      841 tacaatagca cactatatta aacggcaaag ccgtaaaacc ccaacgataa acgaagaagc
      901 agtatatatg gcacaacgag tacagctcac tgcaacggtg tccgaaaacc aactcggtca
      961 acgcttagat caggctttgg ccgaaatgtt cccggattat tcacgttcgc gaataaaaga
     1021 atggatcctc gaccagcgcg tgctggttaa cggcaaagtt tgtgataagc cgaaagaaaa
     1081 agtattgggt ggcgagcagg tggccatcaa cgctgagatt gaagaagaag cgcgttttga
     1141 accgcaggat atcccgctgg atatcgtcta tgaagatgaa gatatcatcg tcattaataa
     1201 accacgcgac ctggtggtac atcctggcgc gggtaacccg gatggtacgg tactgaatgc
     1261 gttgcttcat tattatccgc ccattgccga tgtaccgcgt gcgggcatcg tccatcgtct
     1321 ggataaagac accaccggcc tgatggttgt ggcaaaaacc gttccggctc agacgcgttt
     1381 agtcgaatct ttgcaacggc gtgaaattac tcgtgagtat gaagcggtgg caattggtca
     1441 tatgaccgct ggcggtacag tagatgaacc aatcagccgc cacccgacca aacgtactca
     1501 catggcggtg catccgatgg gtaaaccggc ggtcactcac tatcgcatca tggaacactt
     1561 ccgtgtgcac acgcgtctgc ggttgcgtct ggaaaccgga cgtacgcacc agatccgcgt
     1621 gcatatggcc catatcactc atccgctggt gggcgatccg gtttatggtg gccgtccgcg
     1681 tccgccaaaa ggtgcttcgg aagcgtttat ctccacgctg cgtaagtttg accgccaggc
     1741 gcttcatgca accatgctgc gtctttatca cccgatctcc ggtatcgaaa tggaatggca
     1801 tgcgcctatt ccagaagata tggtggagct gattgaggtg atgcgcgccg atttcgaaga
     1861 gcataaggat gaagtggact ggttatgagt aagctgattg tcccgcagtg gccgctgcca
     1921 aaaggtgttg cggcctgtag ctccactcgt atcggcggcg tgagcttgcc cccgtatgat
     1981 tcgctcaacc tcggtgctca ttgtggcgat aacccggatc acgttgaaga gaatcgtaag
     2041 cgactttttg ctgcgggcaa tttgccttca aaaccggtct ggcttgagca ggtacacggc
     2101 aaagatgtgc ttaagcttac tggcgaacct tatgcctcaa aacgagcaga tgcctcttat
     2161 agcaacacgc cgggcacggt ttgcgcagtg atgactgccg actgcctccc cgtgctgttt
     2221 tgcaatcgag cgggaacgga agtcgccgcc gctcatgctg gctggcgtgg actgtgctca
     2281 ggcgtgctgg aagagacggt ttcctgtttt gctgataatc cggaaaatat tctcgcctgg
     2341 ttagggccgg caattggtcc acgcgcgttt gaagtggggg cggaggttcg cgaggcgttt
     2401 atggcagtag acgccaaagc gagtacagct ttcattcagc atggtgataa gtatctggcg
     2461 gatatttatc tccttgcccg gcagcgtctg gcgaacgtcg gtgtcgaaca aattttcggc
     2521 ggtgaccgtt gtacatatac ggaaaatgag actttcttct cttatcgtcg cgacaagacc
     2581 accggtcgta tggcaagttt catttggctg atataaccta aagaatcaag acgatccggt
     2641 acgcgtgatt ttcttttcac attaatctgg tcaataacct tgaataattg agggatgacc
     2701 tcatttaatc tccagtagca actttgatcc gttatgggag gagttatgcg tctggatcgt
     2761 cttactaata aattccagct tgctcttgcc gatgcccaat cacttgcact cgggcacgac
     2821 aaccaattca tcgaaccact tcatttaatg agcgccctgc tgaatcagga agggggttcg
     2881 gttagtcctt tattaacatc cgctggcatt aatgctggcc agttgcgcac agatatcaat
     2941 caggcattaa atcgtttacc acaggttgaa ggtactggtg gtgatgtcca gccatcacag
     3001 gatctggtgc gcgttcttaa tctttgcgac aagctggcgc aaaaacgtgg tgataacttt
     3061 atctcgtcag aactgttcgt tctggcggca cttgagtctc gcggcacgct ggccgacatc
     3121 ctgaaagcag caggggcgac caccgccaac attactcaag cgattgaaca aatgcgtgga
     3181 ggtgaaagcg tgaacgatca aggtgctgaa gaccaacgtc aggctttgaa aaaatatacc
     3241 atcgacctta ccgaacgagc cgaacagggc aaactcgatc cggtgattgg tcgtgatgaa
     3301 gaaattcgcc gtaccattca ggtgctgcaa cgtcgtacta aaaataaccc ggtactgatt
     3361 ggtgaacccg gtgtcggtaa aactgccatc gttgaaggtc tggcgcagcg tattatcaac
     3421 ggcgaagtgc cggaagggtt gaaaggccgc cgcgtactgg cgctggatat gggcgcgctg
     3481 gtggctgggg cgaaatatcg cggtgagttt gaagaacgtt taaaaggcgt gcttaacgat
     3541 cttgccaaac aggaaggcaa cgtcatcctg tttatcgacg aattacatac catggtcggt
     3601 gcgggtaaag ccgatggcgc aatggacgcc ggaaacatgc tgaaaccggc gctggcgcgt
     3661 ggtgaattgc actgcgtagg tgccacgacg cttgacgaat atcgccagta catcgaaaaa
     3721 gatgctgcgc tggagcgtcg tttccagaaa gtgtttgttg ccgagccttc tgttgaagac
     3781 accattgcta ttctgcgtgg cttaaaagaa cgttacgaac tgcaccacca tgtgcaaatt
     3841 actgacccgg caattgttgc agcggcgacg ttgtctcatc gctacattgc tgaccgtcag
     3901 ctgccagata aagccatcga cctgatcgat gaggcagcat ccagcattcg tatgcagatt
     3961 gactcaaaac cagaagaact cgaccgactc gatcgccgta tcattcagct caaactggaa
     4021 caacaggcgt taatgaaaga gtctgatgaa gccagtaaaa aacgcctgga tatgctcaac
     4081 gaagaactga gcgacaaaga acgtcagtac tccgagttag aagaagagtg gaaagcagag
     4141 aaagcatcgc tttctggtac acagaccatt aaagcggaac tggaacaggc gaaaatcgct
     4201 attgaacagg ctcgccgtgt gggggacctg gcgcggatgt ctgaactgca atacggcaaa
     4261 atcccggaac tggaaaagca actggaagcc gcaacgcagc tcgaaggcaa aactatgcgt
     4321 ctgttgcgta ataaagtgac cgatgccgaa attgctgaag tgctggcgcg ttggacgggg
     4381 attccggttt ctcgcatgat ggaaagcgag cgcgaaaaac tgctgcgtat ggagcaagaa
     4441 ctgcaccatc gcgttattgg tcagaacgaa gcggttgatg cggtatctaa cgctattcgt
     4501 cgtagccgtg cggggctggc ggatccaaat cgcccgattg gttcattcct gttcctcggc
     4561 ccaaccggtg tggggaaaac tgagctttgt aaggcgctag cgaactttat gtttgatagc
     4621 gacgaggcga tggtccgtat cgatatgtcc gagtttatgg agaaacactc ggtgtctcgt
     4681 ctggttggtg cgcctccggg atatgtcggt tatgaagaag gtggctacct gaccgaagct
     4741 gtgcgtcgtc gtccgtattc cgtcatcctg ctggatgaag tggaaaaagc gcatccggat
     4801 gtcttcaaca ttctgttgca ggtactggat gatgggcgtc tgactgacgg gcaggggaga
     4861 acggtcgact tccgtaatac ggttgtcatt atgacctcta acctcggttc cgatttgatt
     4921 caggaacgct tcggtgaact ggattatgcg cacatgaaag agctggtgct cggtgtggta
     4981 agccataact tccgtccgga attcattaac cgtatcgatg aagtggtggt cttccatccg
     5041 ctgggtgaac agcacattgc gtcgattgcg cagattcagt tgaaacgtct gtacaaacgt
     5101 ctggaagaac gtggttatga aatccacatt tctgacgagg cgctgaaact gctgagcgag
     5161 aacggttacg atccggtcta tggtgcacgt ccgctgaaac gtgctatcca gcagcagatc
     5221 gaaaacccgc tggcacagca aatactgtct ggtgaattgg ttccgggtaa agttattcgc
     5281 ctggaagtta atgaagaccg gattgtcgcc gtccagtaat gataaaacga gcccttcggg
     5341 gctcgttttt gtctataaat tagacggaaa agactatgtt taagatgttt tgcctgaaaa
     5401 atgagcgaac gataaagttt ttatattttt t
//
LOCUS       DAZGQL010000190         5307 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.192, whole genome shotgun sequence.
ACCESSION   DAZGQL010000190 DAZGQL010000000
VERSION     DAZGQL010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5307
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.192"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..244)
                     /locus_tag="ACHKIR_004968"
     CDS             complement(<1..244)
                     /locus_tag="ACHKIR_004968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9992580.1"
                     /translation="MLLVLVLIGLNMRPLLTSVGPLLPQLRQASGMSFSVAALLTALP
                     VVTMGGLALAGSWLHQHVSERRSVAISLLLIAVGALM"
     gene            complement(277..747)
                     /gene="cynS"
                     /locus_tag="ACHKIR_004969"
     CDS             complement(277..747)
                     /gene="cynS"
                     /locus_tag="ACHKIR_004969"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008824 - cyanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009439 - cyanate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HFO9992581.1"
                     /translation="MIQSQINRNIRLDLADAILLSKAKKDLSFAEIADGTGLAEAFVT
                     AALLGQQALPADAARLVGAKLDLDEDAILLLQMIPLRGCIDDRIPTDPTMYRFYEMLQ
                     VYGTTLKALVHEKFGDGIISAINFKLDVKKVADPEGGERAVITLDGKYLPTKPF"
     gene            complement(778..1437)
                     /gene="cynT"
                     /locus_tag="ACHKIR_004970"
     CDS             complement(778..1437)
                     /gene="cynT"
                     /locus_tag="ACHKIR_004970"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase CynT"
                     /protein_id="HFO9992582.1"
                     /translation="MKEIIDGFLKFQREAFPKREALFKQLATQQSPRTLFISCSDSRL
                     VPELVTQREPGDLFVIRNAGNIVPSYGPEPGGVSASVEYAVAALRVSDIVICGHSNCG
                     AMTSIASCQCMDHMPAVSHWLRYADSARVVNEARPHPDLPSKAAAMVRENVIAQLANL
                     QTHPSVRLALEEGRIALHGWVYDIESGSIAAFDGATRQFVPLAANPRVCAIPLRQPTA
                     A"
     gene            1547..2446
                     /gene="cynR"
                     /locus_tag="ACHKIR_004971"
     CDS             1547..2446
                     /gene="cynR"
                     /locus_tag="ACHKIR_004971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414872.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CynR"
                     /protein_id="HFO9992583.1"
                     /translation="MLSRHINYFLAVAEHGSFTRAASALHVSQPALSQQIRQLEESLG
                     VPLFDRSGRTIRLTDAGEVWRQYASRALQELGAGKRAIHDVADLTRGSLRIAVTPTFT
                     SYFIGPLMADFYARYPGITLQLQEMSQEKIEDLLCRDELDVGIAFAPVHSPELEAIPL
                     LTESLALVVAQHHPLAACEQVALSRLHDEKLVLLSAEFATREQIDHYCEKAGLHPQVV
                     IEANSISAVLELIRRTSLSTLLPAAIATQHDGLKAISLAPPLLERTAVLLRRKNSWQT
                     AAAKAFLHMALDKCAVVGGNESR"
     gene            complement(2448..2539)
                     /locus_tag="ACHKIR_004972"
                     /pseudo
     CDS             complement(2448..2539)
                     /locus_tag="ACHKIR_004972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2579..3862)
                     /gene="codA"
                     /locus_tag="ACHKIR_004973"
     CDS             complement(2579..3862)
                     /gene="codA"
                     /locus_tag="ACHKIR_004973"
                     /EC_number="3.5.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine/isoguanine deaminase"
                     /protein_id="HFO9992584.1"
                     /translation="MSNNALQTIINARLPGKEGLWQIHLLDGKISAIDAQSGVMPITE
                     NSLDAEQGLVIPPFVEPHIHLDTTQTAGQPNWNQSGTLFEGIERWAERKALLTHDDVK
                     QRAWQTLKWQIANGIQHVRTHVDVSDATLTALKAMLEVKQEVAPWIDLQIVAFPQEGI
                     LSYPNGEALLEEALRLGADVVGAIPHFEFTREYGVESLHKTFALAQKYDRLIDVHCDE
                     IDDEQSRFVETVAALAHREGMGARVTASHTTAMHSYNGAYTSRLFRLLKMSGINFVAN
                     PLVNIHLQGRFDTYPKRRGITRVKEMLESGINVCFGHDDVFDPWYPLGTANMLQVLHM
                     GLHVCQLMGYGQINDGLNLITHHSARTLNLQDYGIAAGNSANLIILPAENGFDALRRQ
                     VPVRYSVRGGKVIASTQPAQTTVYLEQPEAIDYKR"
     gene            complement(3852..5111)
                     /gene="codB"
                     /locus_tag="ACHKIR_004974"
     CDS             complement(3852..5111)
                     /gene="codB"
                     /locus_tag="ACHKIR_004974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015209 - cytosine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HFO9992585.1"
                     /translation="MSQDNNFSQGPVPQSARKGVLALTFVMLGLTFFSASMWTGGTLG
                     TGLSYHDFFLAVLIGNLLLGIYTSFLGYIGAKTGLTTHLLARFSFGVKGSWLPSLLLG
                     GTQVGWFGVGVAMFAIPVGKATGLDINLLIAVSGLLMTVTVFFGISALTVLSVIAVPA
                     IACLGGYSVWLAVNGMGGLDALKAVVPAQPLDFNVALALVVGSFISAGTLTADFVRFG
                     RNAKLAVLVAMVAFFLGNSLMFIFGAAGAAALGMADISDVMIAQGLLLPAIVVLGLNI
                     WTTNDNALYASGLGFANITGMSSKTLSVINGIIGTVCALWLYNNFVGWLTFLSAAIPP
                     VGGVIIADYLMNRRRYEHFATTRMMSVNWVAILAVALGIAAGHWLPGIVPVNAVLGGA
                     LSYLILNPILNRKTTAAMTHVEANSVE"
BASE COUNT         1303 a         1456 c         1412 g         1136 t
ORIGIN      
        1 gcatcaatgc accgacggca atcagtaaca aactgatggc gacgctgcga cgttcgctga
       61 catgctgatg aagccagctt ccggccagcg ccagcccgcc catggtaacc accggcagag
      121 cggtcaacag ggcagccacg ctaaagctca ttccgcttgc ctggcgcaat tgcggtagca
      181 gtggcccgac ggaggtgagc agtggtcgca tattaagacc aatcagcacc agaaccagca
      241 gcatcgtgaa cgtcttttga tggttacgca tggctgtcag aacggtttgg tcggcagata
      301 tttaccatct aaggtgatga ccgcacgttc gccaccctcc gggtccgcca ctttcttaac
      361 gtcgagtttg aagttaatcg cgctgataat gccatcgcca aacttctcat gaaccaacgc
      421 tttcagggtt gtgccgtaca cctgcaacat ttcatagaaa cgatacatcg ttgggtcggt
      481 tggaatacgg tcatcaatgc agccacgcag tggaatcatc tgcaacagta gaatggcgtc
      541 ttcgtcgaga tccagcttcg ctccgaccag gcgggcggcg tcggcaggaa gcgcctgctg
      601 ccccagcaaa gccgcggtta caaaggcttc tgccagaccg gtgccgtcgg caatctcggc
      661 aaatgagaga tcttttttag ctttgctgag caaaatggca tcggcaagat caagacgaat
      721 attgcggtta atttgcgact gaatcatggt ggatctcctg atggtttaaa aataaggtta
      781 cgctgcggtc ggttggcgta gcggtatggc acaaacgcga ggattagcgg ccagtggcac
      841 aaactggcgg gttgcgccgt caaaagctgc gatgctgccg ctttcaatgt cgtagaccca
      901 gccgtgcagg gcaatccgcc cctcttcgag cgccaggcgc accgatggat gagtttgcaa
      961 attagccaac tgagcaatga cgttttcacg taccatcgcc gctgcttttg acggtaaatc
     1021 gggatgcggg cgcgcctcat taacgacgcg ggctgaatcg gcataacgca gccagtggga
     1081 gacggcaggc atatggtcca tgcactgaca gctggcaatg gaggtcatcg cgccacagtt
     1141 ggaatgaccg caaatcacaa tgtcagatac ccgaagtgca gcgacggcat actccaccga
     1201 agcagaaacg ccaccgggtt ccggcccgta ggaagggacg atattgcccg cgttgcgaat
     1261 aacgaacaga tcgccaggct cacgttgcgt caccagctca gggaccagac ggctgtcgga
     1321 gcaggagata aaaagggtgc gcgggctttg ctgtgtcgcc agctgtttaa acaaggcttc
     1381 ccgcttcgga aatgcctcgc gctggaattt aaggaatcca tcaataatct ctttcaccgt
     1441 taacctctgt ctgtctctgg aatgagaggc agactactcg ccgattgtca taaggtaaaa
     1501 gactcattta tgatgagttc tattggattt acttataggt tggcgaatgc tctctcgaca
     1561 tatcaattac tttcttgccg tggctgaaca tggcagcttc acccgtgcag ccagtgcgtt
     1621 gcacgtctcc cagcctgcgc tttcccagca gattcgccag ttagaggaaa gtttgggcgt
     1681 gccgctgttt gaccgtagcg ggcgaacgat tcgtctcact gatgcaggag aagtctggcg
     1741 acagtacgcc agccgggcgt tacaggaact gggggcgggt aaacgggcga ttcatgatgt
     1801 tgccgatctg acgcgaggat cgctgcgtat cgccgtcact cccaccttta caagctactt
     1861 tatcggccct ttaatggcgg atttctatgc gcgctacccc ggcatcacgc tccagctaca
     1921 ggaaatgtcg caggagaaaa tcgaagatct gctttgccgc gacgagttgg acgttgggat
     1981 tgccttcgcg cctgtgcatt cgccggagct ggaggcaatt cctttactga cagaaagttt
     2041 agcgttagtc gtggcgcaac atcatccgct ggctgcctgt gaacaggtgg cgttgagtcg
     2101 cttgcatgat gaaaaactgg tcctgctcag cgcggaattt gccaccagag agcaaattga
     2161 ccactactgc gagaaagcgg ggctacatcc acaggtggtc attgaggcga actcaattag
     2221 cgcggttctg gagctgattc gccgcacttc cctttccaca ttgttgccag cagcgattgc
     2281 cacacaacat gacgggctta aagctatatc ccttgccccg ccactactgg agagaacggc
     2341 ggttttgttg cggcggaaaa atagctggca gacagccgcc gcgaaggcat ttttgcacat
     2401 ggcgttggat aaatgcgcgg ttgttggcgg aaatgaatcg cggtaaccac tgacacaatt
     2461 gcctgatgcg ctacgcttat caggcctacg agttcagtgc tctacgtagg ccggataagg
     2521 cgttcacgcc gcatccggca taaactacgc tcgctttgtc aacacacagc ccagccgatc
     2581 accgtttgta atcgatggct tctggctgct ccagatatac ggtggtttgt gctggttggg
     2641 tgctggcaat caccttaccg ccacgtaccg aataacgtac aggaacctga cggcgcagcg
     2701 cgtcaaaccc attttcagcc ggcaggataa tcaggttggc gctgtttccg gcggcaatgc
     2761 cgtaatcctg caaattcaac gtcctggcac tgtggtgggt gattaaattc aggccatcgt
     2821 taatctgccc gtagcccatc agctggcaaa catgcagccc catatgcagc acttgcagca
     2881 tattcgccgt tcccagcgga taccacggat cgaagacatc atcgtgacca aagcagacgt
     2941 taatgccgga ctccagcatc tctttaacgc gcgtgatgcc gcgacgtttt ggataggtat
     3001 cgaaacgtcc ttgcagatgg atattgacca gcgggttggc gacaaagtta ataccggaca
     3061 ttttcagcaa gcggaacagg cgtgaggtat acgccccgtt ataggagtgc attgccgtgg
     3121 tgtggctggc ggtgactcgc gcgcccattc cttcacggtg cgccagggca gcaacggttt
     3181 cgacaaagcg cgactgctcg tcatcgatct catcacagtg aacgtcgatg agacggtcgt
     3241 atttttgcgc cagggcgaag gttttatgca gcgactccac gccgtattca cgggtaaatt
     3301 caaaatgcgg aatcgccccc actacgtcag cccctaagcg taacgcctct tccagcaacg
     3361 cttcaccgtt gggatacgac aaaatccctt cctgcgggaa ggcgacgatt tgcagatcaa
     3421 tccacggcgc gacttcctgc ttcacttcca gcattgcttt cagcgcagtc agcgttgcat
     3481 ccgaaacatc gacatgggta cgcacatgct gaatgccgtt ggcaatctgc catttcagcg
     3541 tttgccaggc gcgttgtttc acatcgtcat gggttaataa cgctttgcgc tcggcccagc
     3601 gttcaatgcc ttcaaacagc gtgccggact gattccagtt cggttgtccg gcggtttgcg
     3661 tggtgtccag gtgaatatgc ggctccacaa acggcggtat aactaaacct tgttcggcat
     3721 ccaggctgtt ttcagttatg ggcatcaccc cggattgcgc atcaatggcg ctgatttttc
     3781 cgtccagcag atgaatctgc cacagcccct ctttgccggg taaccgggcg ttaataattg
     3841 tctgtaaagc gttattcgac actgttagcc tccacatgcg tcattgcagc cgtcgttttg
     3901 cgattcaaaa tcgggttaag gatcagatag ctcagcgcgc cacctaatac cgcgttgacc
     3961 ggaacaatac ccggtaacca gtggcctgcg gcaatcccca aggcgaccgc cagaatcgcc
     4021 acccaattga cactcatcat acgcgtggtc gcaaagtgct catagcggcg acggttcatc
     4081 agatagtcag cgatgatcac gccccctacc ggaggaatag ctgccgaaag gaaggtcagc
     4141 cagccgacaa aattgttata cagccataat gcgcagaccg taccgataat accgttgatt
     4201 accgaaaggg ttttactcga cataccagta atgttggcga aacctaaacc cgacgcatag
     4261 agcgcgttat cgttggtggt ccagatattc agccccagca ccacaatcgc aggcagcagc
     4321 aggccctgag caatcatcac atcagagata tccgccatcc ccagtgccgc agcgcccgct
     4381 gcaccgaaaa taaacatcaa cgagttgccg aggaaaaagg ccaccatcgc caccagcacc
     4441 gccagtttgg cattacgacc aaaccggaca aagtcagcgg tgagcgtgcc cgcactgata
     4501 aatgacccca caaccagcgc cagcgcgaca ttgaaatcta acggttgtgc gggaacgacc
     4561 gcttttaatg cgtccaggcc gcccatgcca ttaacggcca gccacacgga ataaccgcca
     4621 aggcaggcga tagccggaac cgcaatcacc gaaagaaccg tcagcgccga aatgccaaag
     4681 aagacggtga cggtcatcag taaaccggaa acggcaatca gcaaattaat atccagcccg
     4741 gttgccttgc ccaccggaat ggcaaacatc gccacgccaa cgccaaacca gccaacctgg
     4801 gtgccgccca gcagcagtga aggcagccat gagcctttaa caccaaaaga gaagcgagca
     4861 agaagatgag tggtcaggcc ggtttttgcg ccaatgtaac caagaaatga agtgtaaata
     4921 ccgaggagaa gattaccgat gaggactgcg aggaagaaat catgatagct aagaccggtt
     4981 ccgagagtgc cgccggtcca catactggcg gaaaagaagg ttaatcccag catgacgaac
     5041 gtcaatgcca ataccccttt ccgcgccgac tgcgggactg gcccctggct aaagttgtta
     5101 tcttgcgaca cgaaattccc cctttttgct gtttgaaacc caaaaaatcc gccgcattct
     5161 attcatctga agataaaaag caatcgtttt cgtaggggaa tatatttctt atatgaatga
     5221 gtacaaaaag gcgggggaaa cttatctgtg taaaaacagt gggtaggcat gataagacgc
     5281 gtcagcgtcg catcaggcat ctgcgca
//
LOCUS       DAZGQL010000191         5283 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.193, whole genome shotgun sequence.
ACCESSION   DAZGQL010000191 DAZGQL010000000
VERSION     DAZGQL010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5283
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.193"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..173
                     /locus_tag="ACHKIR_004975"
     CDS             <1..173
                     /locus_tag="ACHKIR_004975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992586.1"
                     /translation="AFCSTSLPASPSGWLFYVRSVKAAMALGRRATGVELETERFEQT
                     VREVQDLASQNG"
     gene            214..289
                     /locus_tag="ACHKIR_004976"
     tRNA            214..289
                     /locus_tag="ACHKIR_004976"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:247..249,aa:Ile,seq:cat)
     gene            297..373
                     /locus_tag="ACHKIR_004977"
     tRNA            297..373
                     /locus_tag="ACHKIR_004977"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:331..333,aa:Arg,seq:tcg)
     gene            387..463
                     /locus_tag="ACHKIR_004978"
     tRNA            387..463
                     /locus_tag="ACHKIR_004978"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:421..423,aa:Arg,seq:tct)
     gene            555..1514
                     /gene="stxA2b"
                     /locus_tag="ACHKIR_004979"
     CDS             555..1514
                     /gene="stxA2b"
                     /locus_tag="ACHKIR_004979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2b subunit A"
                     /protein_id="HFO9992587.1"
                     /translation="MKCILLKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFAHISVPGVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNAMTRDASRAVLRFVTVTAEALRFRQIQREFRLALSETAPVYTMTPEEV
                     DLTLNWGRISNVLPEFRGEGGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNE
                     EIQPECQITGDRPVIRINNTLWESNTAAAFLNRRAHSLNTSGE"
     gene            1527..1790
                     /gene="stxB2b"
                     /locus_tag="ACHKIR_004980"
     CDS             1527..1790
                     /gene="stxB2b"
                     /locus_tag="ACHKIR_004980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2b subunit B"
                     /protein_id="HFO9992588.1"
                     /translation="MKKIFVAALFAFVSVNAMAADCAKGKIEFSKYNENDTFTVKVAG
                     KEYWTNRWNLQPLLQSAQLTGMTVTIKSNTCASGSGFAEVQFN"
     gene            2296..4206
                     /locus_tag="ACHKIR_004981"
     CDS             2296..4206
                     /locus_tag="ACHKIR_004981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFO9992589.1"
                     /translation="MAFKHYDVVRAASPSDLAERITQKLKEGWQPYGSALISTAGYGA
                     EFIQPVVSEGSISSPEEPGNRPTTSAPSVAPEYYYVIALAGQSNGMSYGEGLPLPDTF
                     DSPDPRIKQLARRSTVTPGGAACIYNDIIPADHCLHDVEDMTGLNHPKADLSKGQYGC
                     VGQGLHIAKKLLPFIPANAGILLVPCCRGGSAFTTGTDGTYSDTTGASESSTRWGVGR
                     PLYKDLIGRTKAALAKNPKNVLLAVVWMQGEFDFNGTPANHAARFTEVVDQYRADLAD
                     MAGQCVGGSADSVPWICGDTTYFWKQKNETAYQTVYGSYKNKTEKNIHFVPFMTDENG
                     VNVPTNKPEEDPDIPDIGYYGSKWRTDRSTWTSQDRASHFSSWARRGIISDRLATAIL
                     SCAGKSSAFVNGTAGVVVPDRPVTTSESVIFYDAKKASDNQLKPYGWDGMDGRRTLVD
                     DSGNKALRIEKNNSAKSWSMYCDIAADKAKLLLEKGGEIAVRFKIPENVNLETTRNKY
                     AFGLYWRIAEWPGEGGEGYLSSFFVQTDKASIDVAYHHTVNQQKELGTFGAFDHDWHT
                     LAFKFKGSNSINVTPVLDGVDGQAFDLVKWANTANGLNRFVITDITGSAETYPVLIDT
                     VEVKANKAGAAA"
     gene            4346..4528
                     /locus_tag="ACHKIR_004982"
     CDS             4346..4528
                     /locus_tag="ACHKIR_004982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFO9992590.1"
                     /translation="MIFIHQVMLYFCTAVCVLYLLSGGYRAVRDFWCRQIDKRAAEKI
                     SASQSAGSKPEEPVTP"
     gene            4554..4826
                     /locus_tag="ACHKIR_004983"
     CDS             4554..4826
                     /locus_tag="ACHKIR_004983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFO9992591.1"
                     /translation="MSEITSLVTAEAVKEVLRSEEVRSALKQKLRQNLEARLDAEVDA
                     ILDELLGVQAEPPTEAGDTTAESGEVQPESPVADATEPQPESVMML"
     gene            4903..5118
                     /locus_tag="ACHKIR_004984"
     CDS             4903..5118
                     /locus_tag="ACHKIR_004984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO9992592.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIKEDRRKAARGE"
     gene            5123..>5283
                     /locus_tag="ACHKIR_004985"
     CDS             5123..>5283
                     /locus_tag="ACHKIR_004985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO9992593.1"
                     /translation="MSRKFRYGLSAVVLAMIAAGASAPEILDQFLDEKEGNRTTAYRD
                     GAGIWTICRG"
BASE COUNT         1359 a         1171 c         1422 g         1331 t
ORIGIN      
        1 cagcattttg ctctacgagt ttgccagcct cccccagtgg ctggcttttt tatgtccgta
       61 gcgtcaaagc agcaatggcg ctggggcgtc gtgcaactgg cgttgagctg gagactgaac
      121 gttttgagca gacggtcagg gaagttcagg atttagccag tcagaacgga tgatattgca
      181 ggattagtta cgtaccgtta ttatcctgcg cccggccctt tagctcagtg gtgagagcga
      241 gcgactcata atcgccaggt cgctggttca aatccagcaa gggccaccat cacataccgc
      301 cattagctca tcgggacaga gcgccagcct tcgaagctgg ttgcgcgggg ttcgagtcct
      361 cgatggcggt ccattatctg tacactgcgt tgttagctca gccggacaga gcaattgcct
      421 tctaagcaat cggtcactgg ttcgaatcca gtacaacgcg ccatacttat tttttctggc
      481 tcgcttttgc gggccttttt tatatctgcg ccgggtctgg tgctgattac ttcagccaaa
      541 aggaatacct gtatatgaag tgtatattgt taaaatgggt actgtgcctg ttactgggct
      601 tttcttcggt atcctattcc cgggaattta cgatagactt ttcgacccaa caaagttatg
      661 tctcttcgtt aaatagtata cggacagaaa tatcgacccc acttgaacat atatctcagg
      721 ggaccacatc ggtgtctgtt attaaccaca ccccaccggg cagttatttt gctgtggata
      781 tacgagggct tgatgtctat caggcgcgtt ttgaccatct tcgtctgatt attgagcaaa
      841 ataatttata tgtggccgga ttcgttaata cggcaacaaa tactttctac aggttttcag
      901 attttgcaca tatatcagtg cccggtgtga caactgtttc catgacaacg gacagcagtt
      961 ataccactct gcagcgtgtc gcagcgctgg aacgttccgg aatgcaaatc agtcgtcact
     1021 cactggtttc atcatatctg gcgttaatgg agtttagtgg aaatgccatg accagagatg
     1081 catccagagc agttctgcgt tttgtcactg tcacagcaga agccttacgg ttcaggcaaa
     1141 tacagagaga atttcgtctg gcactgtctg aaactgctcc tgtttatacg atgacaccgg
     1201 aagaagtgga cctcacactg aactggggga gaatcagcaa tgtgcttccg gagtttcggg
     1261 gagagggtgg tgtcagagtg gggcgaatat cctttaataa tatatcagcg atactgggca
     1321 cagtggcggt tatactgaat tgccatcatc agggggcgcg ttccgttcgc gccgtgaatg
     1381 aagagataca accagaatgt cagataactg gcgacaggcc agttataagg ataaacaata
     1441 cgttatggga aagtaatacc gcagctgctt ttctgaatcg cagggctcac tctttaaata
     1501 catccggaga ataacaggag ttaaatatga agaagatatt tgtagcggct ttatttgctt
     1561 ttgtttctgt taatgcaatg gcagctgatt gtgcaaaagg taaaattgag ttctctaagt
     1621 ataatgagaa tgatacattc acagtaaaag tggccgggaa agagtactgg actaaccgct
     1681 ggaatctgca accgctactg caaagcgcac agttaacagg aatgacggta acaatcaaat
     1741 caaatacctg tgcgtcaggt tcaggatttg ctgaagtgca gtttaattaa tatcagaagc
     1801 attgctggtt tcgtggtgtg cagcaatgta gttacagtgt aatcaatgtc acaattcagt
     1861 cagttgaagg ttgtctgccc gactgagaat ttgttaaaaa aatcctgcat ggtgaatccc
     1921 cctgagcgga ggggcatatc agcgtcacag gtgtttctgt tttacctcta tcctttctgt
     1981 gcgggttcag gtgctgatac tgaactcacc gggaggcacc cggcaccatg catgaacggt
     2041 acatagcgca tacaccagcc cctctccgga ggggctttct tgtgggcaaa aaaaagcccg
     2101 agtgggttcg ggcaatagca tgagatatac atttttataa tcgaatggat tttaaccagg
     2161 attcataagg ctgcgcaact gcgcggcctt tttcgtattt cgggctgtag tcttccttct
     2221 gccattgtcc tgtaacttcc ggacttcagc ccgcccctta tctgactcac aacattatcc
     2281 cggccgggag gattcatggc atttaaacac tatgacgtgg tcagggcggc gtcgccgtca
     2341 gaccttgcgg aacgaataac tcaaaaactg aaggaagggt ggcagcctta tggtagtgcg
     2401 ctgatttcga cagctggtta tggtgcggag ttcatccagc cagttgtgag tgaggggagc
     2461 atctcatcac cagaggagcc aggcaaccgt ccgacgacct cagcgccttc tgttgcgcca
     2521 gaatattact atgtgatcgc gcttgctggt cagtccaatg gtatgtcata cggtgaggga
     2581 ctgccattac cggatacatt cgacagccct gatccacgta ttaaacagtt agcgcgtcgc
     2641 agtacggtga caccgggagg tgctgcatgt atctataacg atattatccc ggcagatcac
     2701 tgtctccatg atgtggagga tatgacaggg cttaatcatc ccaaagcgga tttatcgaaa
     2761 ggtcagtacg gctgtgtggg acaggggctg catatcgcca aaaagcttct gccatttata
     2821 ccggcgaatg cgggcattct tcttgttccg tgctgccgtg gtggttcagc tttcaccacc
     2881 ggaaccgatg gcacatacag tgacactact ggcgcctcag agagttccac ccgctggggg
     2941 gtgggcaggc cgctgtataa ggacctcatt ggtcgtacaa aagccgcgct ggcgaagaat
     3001 ccgaaaaatg tgctgcttgc cgtggtatgg atgcaagggg aatttgattt taacggaacg
     3061 ccagcgaatc atgcagcccg ttttacagaa gtggtggatc aataccgtgc ggaccttgca
     3121 gatatggcgg ggcagtgtgt gggtggctca gctgacagtg ttccctggat ttgtggagac
     3181 acaacatatt tctggaagca gaagaacgaa acggcgtacc agacggtgta tggtagttac
     3241 aaaaataaaa cggaaaagaa tatccatttc gtgccgttca tgaccgatga gaacggggtg
     3301 aatgtgccga cgaacaaacc ggaagaagac ccggatattc cggatatcgg gtattacggt
     3361 tcaaaatggc gaacggacag aagcacctgg acatctcagg acagggccag tcatttcagt
     3421 tcatgggctc gccgtgggat tatttccgac cgtctggcaa cggcgattct gagctgcgcg
     3481 ggtaagtctt ctgcgtttgt taatggtact gccggggtgg ttgttccaga cagaccggtt
     3541 accacctcag agtctgtaat tttttacgat gccaaaaaag cttcagacaa tcagctgaaa
     3601 ccttatggct gggacggtat ggatggcaga cgcacactgg ttgatgacag cggcaataaa
     3661 gctctgcgaa ttgagaaaaa taacagcgcg aaatcctggt caatgtactg tgatattgct
     3721 gcagacaagg caaaactttt actggaaaaa ggcggggaaa ttgctgtccg gtttaaaatc
     3781 cccgaaaacg tcaatcttga gacaaccaga aacaagtatg cctttggttt gtactggcga
     3841 atagcggaat ggccgggtga gggtggtgaa ggctatctga gttctttctt tgtccagaca
     3901 gataaagcca gtattgatgt tgcataccat catacagtta atcaacaaaa agaacttggc
     3961 acgtttggcg cattcgacca tgactggcat acgcttgcat ttaaatttaa gggcagtaac
     4021 agcattaatg ttactccggt gcttgatggt gtggatggac aggcgtttga cctggtgaaa
     4081 tgggccaata ctgctaatgg actcaacagg tttgtcatta cggatattac aggtagtgca
     4141 gaaacctacc ctgtacttat tgatacggtg gaagttaaag caaacaaagc tggagcagcc
     4201 gcataattgc taaaaaaagc cgccagcggc aggaatggaa gctggcggag gtaatcccaa
     4261 tggagaatgt aaagaaaaga tgctttcgta cattggtttt ttaaatgaaa acagttctca
     4321 ttgtcaacca taacggtaag aaattatgat atttattcat caggtgatgc tgtacttctg
     4381 tacggcggtc tgtgtgctgt atcttctttc gggtgggtac agggctgtgc gcgatttctg
     4441 gtgcaggcag attgataaaa gggccgctga gaaaatcagc gccagtcagt cagccggaag
     4501 caaacccgaa gagcccgtta ctccttaaca acccctttca gcgagaaaat cccatgtcag
     4561 aaatcacatc cctggtcact gcagaggcag tgaaggaagt cctgcgctct gaagaagtcc
     4621 ggagcgcact gaaacagaaa cttcgccaga atcttgaggc gcgtcttgat gcagaagttg
     4681 atgccattct ggatgagctg cttggtgtac aggcagagcc accgactgaa gcgggagata
     4741 ccaccgcaga gagcggtgaa gttcagcctg aatcaccggt cgccgatgcg actgaacctc
     4801 aacccgaatc ggtcatgatg ctgtaacgag gagtcagggc catcagtaaa cggctgctgg
     4861 cctttttcat gttgtgagct tccggattgc gggagacggg gtatgtacca gatggaaaaa
     4921 atcacaacag gtgtgtcata caccacgtca gcggtgggaa cgggctactg gttcctgcag
     4981 ttgctggaca gggtttcccc gtctcagtgg gcggcaatag gcgtgctggg gagtctgctg
     5041 tttgggctgc tgacatatct gacgaacctg tatttcaaaa ttaaagaaga ccggcgtaag
     5101 gcggcgcggg gagagtaagc tgatgagcag gaaattccgc tatggtttat cagcggtcgt
     5161 tctggcgatg attgctgcag gtgcttctgc gcctgaaatc ctcgaccagt ttcttgacga
     5221 aaaagaaggt aatcgcacca cagcataccg tgatggtgcg ggtatctgga ccatctgccg
     5281 tgg
//
LOCUS       DAZGQL010000192         5234 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.194, whole genome shotgun sequence.
ACCESSION   DAZGQL010000192 DAZGQL010000000
VERSION     DAZGQL010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5234
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.194"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            11..3037
                     /locus_tag="ACHKIR_004986"
     CDS             11..3037
                     /locus_tag="ACHKIR_004986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFO9992594.1"
                     /translation="MAVKISGVLKDGTGKPVQNCTIQLKAKRNSTTVVVNTLASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSRPGTLNDFLGAMTEDDARPEA
                     LRRFELMVEEVARNASAVAQNTAAAKKSASDASTSAREAATHATDAADSARAASTSAG
                     QAASSAQSASSSAGTASTKATEASKSAAAAESSKSVAATSAGAAKTSETNAAASEQSA
                     ATSASTATTKASEAATSARDAAASKEAAKSSETNASLSASSAASSATAAGNSAKAAKT
                     SETNARSSETAAGQSASAAAGSKTAAASSASAASTSAGQASASATAAGKSAESAASSA
                     STATTKAGEATEQATAAARSASAAKTSETNAKASETRAESSKTAAASSASSAASSASS
                     ASASKDEATRQASAAKGSATTASTKATEAAGSAKAAAQSKSTAESAATRAETAAKRAE
                     DIASAVALEDASTTKKGIVQLSSATNSTSESLAATPKAVKAAYDLASGKYTAQDATTA
                     QKGIIQLSSATNSTSETLAATPKAVKAANDNAEKRLQKDQNGADIPGKDTFTKNIGAC
                     RAFGGSVSTTTGNWTTAQFIEWLDSQGAFNHPYWMCKGSWSYGNNKIITDTGCGNIHL
                     AGAVIEVMGIKSAMTIRITTPTTSSGGGTTNAQFTYINHGTDYSPGWRRDYNSRNKPT
                     ASEIGALPSGGTAVSSVNLASKGRVAALTDNTQGAAGLELYEVYNNGYPTTYGNIIHL
                     KGMTAVGEGELLIGWSGTSGAHAPAFIRSRRDTTDANWSPWAQLYTSAHPPAEFYPVG
                     APIPWPSDTVPSGYVLMQGQTFDKSAYPKLAAAYPSGVIPDMRGWTIKGKPASGRAVL
                     SQEQDGIKSHTHSASASSTDLGTKTTSSFDYGTKSTNNIGAHTHSVSGTAASAGNHTH
                     SVTGASAVSQWSQNGSVHKVVSAASVNTSAAGAHTHSVSGTAASAGAHAHTVGIGAHT
                     HSVAIGSHGHTITVNAAGNAENTVKNIAFNYIVRLA"
     gene            3034..3936
                     /locus_tag="ACHKIR_004987"
     CDS             3034..3936
                     /locus_tag="ACHKIR_004987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000631340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HFO9992595.1"
                     /translation="MITLILSAPVTEMAEAFKRVFANADNVNIVGKPFETIREFDCMV
                     SAANSFGLMDGGVDAAITTFFGSQLQARVQNHILREYLGEQPVGSAFVIETGHNYHPW
                     LVHAPTMRVPLTIDGTDAVYNATWAALLAIFQHNKNATTDRKIKTVVFPAMGAGCGQV
                     PFESVARQMKQAWDNFNKKTESINWEYAQSRQSAVFGTYAYCPGNSVCRYADTKYIGC
                     GDYRTYCSRSGQVCINPEHQADDVLIQHQANNRFRPGSHIHRINPENPVGNVISGAHS
                     HGSSIVIGAHTHTLNKQYSVSDIK"
     gene            3945..4520
                     /locus_tag="ACHKIR_004988"
     CDS             3945..4520
                     /locus_tag="ACHKIR_004988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO9992596.1"
                     /translation="MDFRMSEQPRTITIYNLLAGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSDESSWHLVEDHRGKTVYDVASGDALFISELGSLPENVTWLSP
                     EGEFQKWNGTAWVKDTEAEKLFRIREAEETKNSLMQVASEHIAPLQDAVDLEIATEKE
                     TLLLEAWKKYRVLLNRVDTSTAPDIEWPALP"
     gene            complement(4618..5208)
                     /locus_tag="ACHKIR_004989"
     CDS             complement(4618..5208)
                     /locus_tag="ACHKIR_004989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO9992597.1"
                     /translation="MSRIYAYCRVSTLEKTTKNQRREIESAGFNIKSQQIIEEQISGS
                     AATSERPGFNQLLDRLKRGDALIVTKLDRLGCNPMDISKTVEQLTKAGIKVHCLALGG
                     VDLTSPPGKMMMQVISAVAEFERDLLLERTHSGILRARGAGKRFGRPPVLNDEQIQRV
                     LEQIKSGINISDIAQEFKTSRQTILRAKAKNQTPDI"
BASE COUNT         1412 a         1298 c         1382 g         1142 t
ORIGIN      
        1 tggggtgaat atggcagtaa agatttcagg tgtactgaaa gacggcacag gaaaaccggt
       61 acagaactgc acaatccagc tgaaagcaaa acgtaacagc accacggtgg tggtgaacac
      121 gctggcctca gaaaatccgg atgaagccgg gcgttacagc atggacgttg agtacgggca
      181 gtacagcgtt attctgttgg tggagggatt cccgccgtca catgccggga ccatcaccgt
      241 gtatgaagat tcccgacccg gtacgctgaa tgattttctc ggtgccatga cggaggatga
      301 tgcccgtcct gaggcactgc gccgttttga actgatggtg gaagaggtgg cgcgtaacgc
      361 gtccgcggtg gcacagaaca cggcagccgc gaagaagtca gccagcgatg ccagcacatc
      421 agcccgtgag gcggcaaccc atgcgactga tgctgcagat tcagcacgtg cagccagcac
      481 gtcagccgga caggccgctt cgtcggctca gtcagcgtct tccagcgcag gaacggcatc
      541 aacaaaggcc actgaagcct caaaaagtgc tgccgctgca gagtcctcaa aaagcgtggc
      601 agctaccagt gccggtgcgg cgaaaacgtc agaaacgaat gccgcagcgt cagaacaatc
      661 agccgccact tctgcatcca ccgcgaccac gaaagcgtca gaagctgcca cctcagcccg
      721 ggatgcggcg gcctcaaaag aggcagcgaa atcatcagaa acgaacgcat ccttgagcgc
      781 cagtagcgca gcttcctcgg caacggcggc aggaaattcc gcgaaggcgg caaaaacgtc
      841 cgagacgaac gccaggtctt ctgagacggc agcgggacag agcgcctcag ctgcggcagg
      901 ctcaaaaaca gcggctgcgt cgtctgccag tgccgcgtca acaagtgccg ggcaggcctc
      961 agccagtgcc accgccgccg gaaaatcggc agaaagcgcc gcatcatccg cttcaacagc
     1021 cacaacgaag gctggcgaag ccactgagca ggccacggca gcagcgaggt cagcttccgc
     1081 agcgaagaca tccgaaacga acgcgaaagc gtcggaaacc cgtgcagaat cctcaaaaac
     1141 ggcagcggca tcgtccgcca gttcggcggc gtcatcggca tcatcggcgt ctgcttcaaa
     1201 agatgaggcg accagacagg cgtcagcagc gaagggcagc gccacgacgg catccacgaa
     1261 ggcgacagag gctgctggta gtgcgaaggc agcagctcag agcaaaagta cggcggaatc
     1321 tgcagcaacg cgcgctgaga cagcggcaaa acgggcagag gatattgcat ccgccgtggc
     1381 gcttgaggat gcgagcacga cgaaaaaggg gatagtacag ctcagtagtg cgactaacag
     1441 cacttccgag tcactggcgg caacaccaaa agccgttaag gccgcgtatg acctggctag
     1501 cgggaaatac accgcacagg atgcaacgac agcacagaaa gggataatcc agctaagcag
     1561 cgcgaccaac agcacgtctg aaacgctggc ggcaacgcca aaggcagtaa aagcagccaa
     1621 tgacaatgct gagaaacgtc tgcagaaaga tcagaacggt gcggatatcc ctggcaaaga
     1681 cacctttacg aaaaatattg gtgcctgccg tgccttcggt gggtcagtaa gcacaacaac
     1741 aggaaactgg acgactgcac agtttatcga gtggctggat tctcagggag catttaacca
     1801 tccatactgg atgtgcaagg gttcctggtc ttatggcaat aataaaatca ttactgatac
     1861 tggctgcggt aatattcatc tcgccggagc tgtcattgaa gtaatgggga taaagtcagc
     1921 gatgacgatc cgcattacca ctccgaccac atccagcggt ggcggaacaa ccaacgccca
     1981 gtttacctat attaatcacg gaacagatta ttcacctggc tggcgaaggg actataactc
     2041 cagaaataag ccaacggcat cagagatcgg ggcgctaccg tcaggtggaa cagcagtatc
     2101 atcagttaat ctggcttcaa aaggtcgggt agccgcgctg acagacaata cgcagggggc
     2161 agcaggtctt gagttatacg aggtgtataa caacggatat ccaacaacgt atggaaatat
     2221 cattcacctg aaagggatga cagccgttgg cgaaggtgag ttactcatcg gctggagtgg
     2281 tacaagcggt gctcatgctc cggcatttat tcgttcacga cgggatacga ccgacgcaaa
     2341 ctggtcgccg tgggcgcagc tttacacctc ggctcatcct cctgcagagt tttatccagt
     2401 cggtgcacca atcccgtggc catcagatac cgttccgtct ggttatgtcc tgatgcaggg
     2461 gcagactttt gacaaatctg catacccgaa acttgcagcc gcttatccgt caggcgtgat
     2521 ccctgatatg cgtggctgga cgattaaggg caaacctgcc agtggtcggg ccgtattgtc
     2581 tcaggaacag gacggcatta aatcgcacac ccacagcgcc agcgcatcca gtacggattt
     2641 ggggacgaaa accacatcgt cgtttgatta cggcactaaa tccacgaata acatcggggc
     2701 gcatacgcac agtgtgagcg gtacagccgc aagtgccgga aaccatactc atagtgtcac
     2761 aggcgcatca gcagtcagcc agtggtcaca aaatgggtca gtacataagg tagtgtctgc
     2821 ggccagtgtg aatacaagtg ctgcaggagc gcacactcat agtgtcagcg gcacagctgc
     2881 atctgcaggt gctcacgcac atactgtcgg tattggtgct catacgcact cggttgcgat
     2941 tggctcacat ggacacacca tcaccgttaa cgctgctggt aacgcggaaa acaccgtcaa
     3001 aaacatcgca tttaactata ttgtgaggct tgcatgatta cgctcattct ttctgcacca
     3061 gtaaccgaaa tggctgaagc atttaagcgg gtatttgcaa atgcagataa tgtgaatatt
     3121 gtcggaaagc catttgaaac aatcagagaa tttgactgca tggtaagtgc ggcaaatagt
     3181 ttcggcctga tggatggcgg tgttgatgcc gccattacca cattcttcgg ttctcagcta
     3241 caggcccgcg ttcagaatca tattcttcgt gaatatctcg gcgagcagcc tgtaggttct
     3301 gcatttgtta ttgaaacggg gcataattat cacccctggc tggtacatgc gccaacaatg
     3361 cgtgttccgt tgacaattga cggaacagac gctgtatata acgcaacttg ggcagctcta
     3421 cttgctatct ttcagcacaa taaaaacgca acgacagaca ggaaaataaa gacggtggta
     3481 ttccctgcaa tgggagccgg atgtggtcag gtgccgtttg aaagcgttgc ccggcagatg
     3541 aagcaggcat gggataactt taataaaaaa acagaatcaa ttaactggga atacgcacaa
     3601 tcccgccagt cggcagtatt tggcacatat gcatactgtc cgggtaattc tgtttgccgt
     3661 tatgcggata ctaaatatat tggatgcggc gattatcgga cgtattgctc acgttccggg
     3721 caggtctgta ttaaccctga acatcaggct gatgatgtac tgatacaaca tcaggctaat
     3781 aaccggtttc gccctggttc gcatatacac cggattaatc cagaaaatcc tgtaggtaat
     3841 gtcatctctg gcgcacatag ccacggaagt agcatcgtta ttggtgctca cacccatacg
     3901 ctcaataaac aatattctgt ctctgatatt aagtagaggt gaacatggat ttcagaatga
     3961 gtgaacaacc acggaccata acaatttata atctgctggc cggaactaat gaatttattg
     4021 gtgaaggtga cgcatacatt ccgcctcata caggtctgcc agcaaacagt accgatattg
     4081 caccgccaga tattccggct ggtttcgtgg ctgttttcaa cagtgatgag tcatcgtggc
     4141 atctcgttga agatcatcgg ggtaaaacgg tttatgacgt ggcttccggc gacgcgttat
     4201 ttatttctga actcggctca ttaccggaaa atgtcacctg gttatccccg gaaggggagt
     4261 ttcagaagtg gaacggcaca gcctgggtga aggatacgga agcagaaaaa ctgttccgga
     4321 tccgggaggc ggaagaaaca aaaaacagcc tgatgcaggt agccagtgag catatagcgc
     4381 cgcttcagga tgctgtggat ctggagatcg caacggagaa agaaaccttg ttgctggaag
     4441 cctggaaaaa gtatcgggtg ttgctgaacc gtgttgatac atcaactgca ccggatattg
     4501 aatggccagc cctaccgtag ggtaagatat ataaattcta taattagaag tatctttcca
     4561 tttaaggcta ggaagggggg cttggaaaac gtaaggaatc tcacaccgag attattttta
     4621 tatatcaggt gtctgatttt ttgctttagc tcttaaaatg gtttgccgcg aggttttgaa
     4681 ttcttgggca atgtcactta tatttatacc tgacttaatt tgctctagta ccctctgtat
     4741 ttgttcatca tttaacacag gtgggcgacc aaatcgcttt cctgcgcccc gtgctcttaa
     4801 tatccctgaa tgagtacgct caagtaaaag gtctcgctca aattcagcga ctgctgaaat
     4861 tacttgcatc atcatttttc caggtggact ggtcaggtca acgcccccca atgctaagca
     4921 atgcactttg atacctgctt tggtcagttg ttccaccgtt ttactgatat ccattggatt
     4981 acaaccgagg cgatccagtt ttgtgacaat caatgcatca cctcttttca gtcgatcaag
     5041 caactggtta aaaccaggac gctcactggt tgctgctgag ccgctaattt gttcttcaat
     5101 tatttgctga gatttgatgt taaaacctgc actttcgatt tcccgacgtt gatttttggt
     5161 tgtcttttcc agagttgata cccgacagta agcataaatt cgagacatag tgagatcttc
     5221 tatacgaaat tggt
//
LOCUS       DAZGQL010000193         4794 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.195, whole genome shotgun sequence.
ACCESSION   DAZGQL010000193 DAZGQL010000000
VERSION     DAZGQL010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4794
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.195"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(271..>4794)
                     /locus_tag="ACHKIR_004990"
     CDS             complement(271..>4794)
                     /locus_tag="ACHKIR_004990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD38 domain-containing protein"
                     /protein_id="HFO9992598.1"
                     /translation="LDNYFGRYDVSNDGKITSGGDFMTEAQRVRRIFKDGRYVDAEYP
                     VRQEVYDAFKGVIQAIKNSDMPRRSALLDEVRSKPYWSTDVEMAARAFERYVQDKARM
                     AGVENDYLVNIRKAPEHNTDNTWAYPTNAELDGGIRDAFDHLFSTLKTRETDKGVAFY
                     SRKGVTRTPEGNLISDVNRSAAEAKGSPVPQVEAVARGVMSGIKDSDLKVRVVKSQKE
                     AEALAGESFDGYGRVHAFYRPDKREIVLVADNIPDGRTVREKIRHEIIHHAMEHVVTP
                     AEYQTIIKTVLKTRDSDNATIREAWRKVDASYGKESPEVQAGEFLAHMAEKQPNKFVA
                     AWERVVALVKGVLRRTGLLKPTELNDIRLVRETIRTLGQRVREGYTPREDGAGASSQY
                     SRSGKPDPFKADAYDAKQFASEVRRIAGLQSVPRGGEVRVGDTPPVLRALGAPNKELV
                     MPAHVVHKATRPELREHYVSVETMADLPQLLADPVAVMTSKTEHDSLVTLIEAKDRRG
                     APVIVAIHMRAKGAVAEVNKIASVYGKDNRIALQEQLDTSLLYLNKEKAADWLLSAGL
                     QLPGANTINGSSGFKVLHPEDIRKGAYYSRTSSLTPEETIASRFVRQMQDKFQVLKAV
                     QENIRKTGGKIDDSNNAYMAEELFHGKAENDLNVMKERYVQPLAKLLANYKISQADLD
                     EYLYARHAPERNAHIAKINPKMPDGGSGMTNAEAAEIMQRVRNSGKQAQYDRLAGIVD
                     DMLARRRELIREAGLEENGVVDAWQNAYRYYVPLKGQDVDGVVSLPRTGKGFTIGGRE
                     SRQAMGRASRAQSPSTQAIQDLSESLIRHRKNEVGNAFLKLVQDNPDKDYWQVFTDDR
                     PDTMRTIAERKDQETGETRREVVERPVPMAMMADRYFTTKKNGKTYYIKLHDPRLMRA
                     MKNMGPETSNAVIRTLGKVNRFLATVNTSYNPEFLVSNFIRDVQTAVMNLKAEQGRSD
                     GKLKGLDNLSALAVVKDSRSAMSAVYASLRGKTLTGKGAQWQKVWKEFVEDGGKTGWF
                     NMGDLEGQQKEMDRLVSLAKGGWKGQSIGAWNSFLNLVEDANGAVENALRLSAYKHAR
                     DAGLSRQQAASLAKNMTVNFNRRGEQGALMNSLYMFANASIQGTANLVRTLGHLNGEG
                     PLLERLRWKNLNVPQKIALAAVGAGYLLGSLNRSVAGEDDDGVNWYDKVPSHVKERNL
                     VIMKSVFGGKAGEYWSIPLPYGYNVFFLLGHTAEGVAAGDLTASRAAGNVVGGVLGAF
                     SPIGSETSETLSGALLKNAAPTILRPFANLAMNENFMGAQIYQENMPFGTPKPDSQLG
                     RRSTPEAYKAFASWLNAFSGGSQYRPGAVDVTPESLKYWIDYISGGTGRFISKTTDAA
                     VKSLNGIDIPEQQVPFLGKISGEVMPYADQQKMYDRMTEVAQYHAELKSLTGAERTAF
                     IDENNGKLSMNGLMQDTRKRLKDLRKQRDAIYADSTLSLAQQSAMVKSVERDMKIAVD
                     RFNREYNKKVGVE"
BASE COUNT         1045 a         1430 c         1091 g         1228 t
ORIGIN      
        1 gatattccga aaataacccc aaaatattaa tttaatgttt taggatttgt cttacattat
       61 tcttggtgta gtcactcaga accacgacta caccaccgga acaggcatca gaaaaaactt
      121 actctcatca ggcattctgt ttcacatctg cttacactgt aatttctgat caatatcaca
      181 acattatccg aatttaactg ttcagttcat ctacaatatt tccctgcgtg actttatatc
      241 agccccgcac ttccgtacgg agcatgttct ttactccact cccacttttt tgttgtactc
      301 gcggttaaac cgatccacgg caattttcat atcccgctct accgatttca ccatcgccga
      361 ctgttgcgcc agactgagag tactgtcggc gtaaatggca tcacgctgtt tacgcaaatc
      421 cttcagtctc ttccgggtat cctgcataag cccgttcatc gacaattttc cgttgttctc
      481 gtcaatgaac gccgttcttt ctgcaccggt cagactcttc agctctgcgt ggtactgcgc
      541 aacctctgtc atccggtcgt acatcttctg ctggtcagca tacggcatca cctcacctga
      601 aattttcccc aggaaaggca cctgctgctc cggtatatca ataccattca gcgatttcac
      661 cgccgcatcc gtggttttgg aaatgaagcg ccctgtacca ccagagatat agtcaatcca
      721 gtatttcagt gattccggcg tgacatccac cgcgccggga cggtactggc tgccacctga
      781 gaacgcattc agccaggatg caaacgcctt gtacgcttct ggcgttgaac gtcttcccag
      841 ctggctgtca ggttttggtg taccaaacgg catgttctcc tggtaaatct gcgcccccat
      901 gaagttttca ttcatggcaa ggttcgcaaa cggacgcaga atggtcggcg ctgcattttt
      961 cagcaatgcc ccggacagtg tttccgacgt ctcactgcca atcgggctga atgcaccaag
     1021 cacaccacca acaacattac cggcagcacg ggacgccgtc aggtcacccg ccgccacacc
     1081 ttcagcggta tgcccgagca ggaagaaaac gttgtacccg taaggcagag gaatactcca
     1141 gtactctccg gccttgcccc cgaacaccga tttcataatg acgaggttac gctctttcac
     1201 atgagacggc accttgtcat accagttaac cccgtcatca tcctcacccg ccacactgcg
     1261 gttaagcgag ccaagcagat aacctgctcc cacagcggca agcgcgattt tctgcggaac
     1321 attcagattc ttccagcgaa ggcgctccag taaaggtccc tcaccattaa gatgtccgag
     1381 cgttctcacc agatttgccg ttccctgaat actggcgttg gcgaacatgt acagcgagtt
     1441 catcagcgcc ccctgctcac cacgacggtt aaagttcacc gtcatgtttt tggcaagaga
     1501 cgccgcctgc tggcgtgaca aaccggcatc acgggcgtgt ttataagctg acagacgcag
     1561 tgcgttttca accgcgccgt tggcatcctc gacaaggtta aggaacgaat tccatgcacc
     1621 gatactctgg cctttccatc ctcccttcgc cagcgaaaca aggcgatcca tttccttctg
     1681 ctggccttca aggtcaccca tgttaaacca accggtttta cctccgtcct caacaaactc
     1741 tttccacacc ttctgccact gtgcaccttt gcccgtgagg gttttaccac gcagactggc
     1801 gtatacggct gacatggcag aacggctgtc tttgactaca gccagggcgg ataagttatc
     1861 cagccctttc agtttaccgt cgctccttcc ctgctcagcc ttcaggttca tcaccgccgt
     1921 ctgcacgtca cggatgaagt tactgaccag gaattccggg ttatacgacg tgttcaccgt
     1981 tgccaggaag cggttaactt tccccagcgt acggattacg gcgttactgg tttccggtcc
     2041 catattcttc atcgcacgca tcaggcgcgg gtcatggagt ttgatgtagt acgttttgcc
     2101 gttctttttg gtggtgaagt agcggtctgc catcattgcc atcggtacag ggcgttcgac
     2161 aacttcgcga cgggtttcac cagtttcctg gtccttgcgc tctgcaatcg tccgcatggt
     2221 atccggtctg tcatcggtga atacctgcca gtaatccttg tcgggattat cctgcaccag
     2281 tttcaggaag gcgttaccca cttcgttttt gcgatggcgg atcagcgatt cgctcaggtc
     2341 ctgtatcgcc tgagtggacg gagactgtgc acgggatgca cgccccatgg cctgcctgct
     2401 ttcacgtccg ccgatggtga agcccttacc tgtacgggga agtgacacca caccgtcaac
     2461 atcctgacct ttcaggggaa cgtagtaacg gtaggcgttc tgccaggcat ccaccacacc
     2521 gttctcttca agtccggcct cacggataag ctcacggcga cgggccagca tatcgtcaac
     2581 aatccctgcc agacggtcat actgtgcctg tttgccgctg ttacgcacac gctgcatgat
     2641 ttccgccgcc tccgcgttgg tcatccccga accgccgtcc ggcattttag ggttgatttt
     2701 cgcaatatgt gcgttacgtt ccggcgcgtg acgggcgtag aggtactcat ccagatcggc
     2761 ctgagagatt ttgtagttcg ccagcaattt ggccagcggc tgaacgtagc gctccttcat
     2821 tacgttcagg tcgttttccg ccttcccgtg gaagagttct tcagccatat aggcgttatt
     2881 gctgtcatct atttttccgc cagttttacg gatattctcc tgcaccgcct tcagcacctg
     2941 gaatttatcc tgcatctggc gaacaaaacg cgatgcaatg gtctcttccg gtgtcagact
     3001 gctggtgcgg gagtaatacg cccccttgcg aatatcttca ggatgtagta ctttaaatcc
     3061 agaagagccg ttgatggtgt tcgccccggg caattggagc ccggctgaaa gaagccagtc
     3121 agcggctttt tctttattca gatacagcaa ggaagtatcc aactgttcct gcaaagcgat
     3181 cctgttgtcc tttccgtaga cactggcaat cttattaacc tcagctaccg cccctttagc
     3241 acgcatgtga attgcaacaa tgacaggtgc acctcttctg tccttagcct caatcaaagt
     3301 taccagtgag tcatgttccg tctttgatgt catcaccgca accgggtcgg caagcaattg
     3361 cggtaagtca gccatcgttt caacagaaac atagtgctca cgtaattctg gtctggtggc
     3421 cttgtgaacc acatgagctg gcataaccag ctctttattt ggtgcgccaa gtgctcttaa
     3481 cactggcgga gtgtcaccaa cccgcacctc tccacctcgt ggaaccgatt gtaatccggc
     3541 aatgcgacgc acttctgaag caaactgctt tgcatcatag gcgtcagcct taaacggatc
     3601 cggcttacca ctacgggagt actgagacga tgcgcccgcg ccatcctcac gcggcgtgta
     3661 accttcccgc acacgctggc ctaacgtacg gatggtctcg cgaacaagtc tgatatcgtt
     3721 cagttccgtc ggcttcagta accccgtacg acgcagtacc cctttgacca gggcaacaac
     3781 acgctcccat gccgccacga atttattcgg ctgtttctcc gccatatgtg ccagaaattc
     3841 acccgcctgt acttccggtg attccttacc ataggacgca tcaaccttac gccaggcttc
     3901 acggatggtg gcgttatcac tgtcgcgggt tttcagcacg gttttgataa tcgtctgata
     3961 ttccgctggc gtgacaacat gctccatggc atggtgaatt atctcgtgac gtatcttctc
     4021 gcgtacggtc cgcccgtcag ggatgttatc cgccaccagg acaatttcac gtttatccgg
     4081 acgatagaac gcgtgcaccc tgccgtaacc gtcgaacgat tcacccgcca gcgcttcagc
     4141 ctctttctgt gacttcacca cacggacctt caggtcactg tccttaatgc cgctcatcac
     4201 gccacgggca accgcttcaa cctgcgggac cgggctgcct ttggcttccg ccgcactacg
     4261 gttaacatcc gaaatgagat tgccttcagg tgtgcgggtt acgcccttac gggaataaaa
     4321 cgcaacgccc ttgtccgtct cacgggtttt cagcgtactg aacaggtgat cgaatgcatc
     4381 acgaataccg ccatccagtt ccgcattcgt cggataagcc caggtgttat ctgtgttgtg
     4441 ctcaggtgcc ttacggatat tgaccagata atcattctcc acgccagcca tacgcgcctt
     4501 atcctgaaca taacgctcaa aggcacgcgc cgccatttca acatccgttg accagtacgg
     4561 ttttgagcgc acctcatcaa gaagcgctga acgacgcggc atgtcactgt ttttaatggc
     4621 ctgaatcaca cctttaaaag cgtcgtaaac ctcctgacgt accggatatt cagcatcaac
     4681 atacctgccg tctttaaata tgcgcctgac acgctgtgct tccgtcataa agtcgccacc
     4741 tgacgtaatt ttcccgtcat tggaaacgtc ataacgacca aaataattat ccag
//
LOCUS       DAZGQL010000194         4603 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.196, whole genome shotgun sequence.
ACCESSION   DAZGQL010000194 DAZGQL010000000
VERSION     DAZGQL010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4603
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.196"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..593)
                     /locus_tag="ACHKIR_004991"
     CDS             complement(<1..593)
                     /locus_tag="ACHKIR_004991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFO9992599.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQ
                     YDFPGDDTPIVRGSALKALEGDAEWEAKILELAGFLDS"
     gene            complement(708..783)
                     /locus_tag="ACHKIR_004992"
     tRNA            complement(708..783)
                     /locus_tag="ACHKIR_004992"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(748..750),aa:Thr,seq:ggt)
     gene            complement(790..864)
                     /locus_tag="ACHKIR_004993"
     tRNA            complement(790..864)
                     /locus_tag="ACHKIR_004993"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(829..831),aa:Gly,seq:tcc)
     gene            complement(981..1065)
                     /locus_tag="ACHKIR_004994"
     tRNA            complement(981..1065)
                     /locus_tag="ACHKIR_004994"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1029..1031),aa:Tyr,seq:gta)
     gene            complement(1074..1149)
                     /locus_tag="ACHKIR_004995"
     tRNA            complement(1074..1149)
                     /locus_tag="ACHKIR_004995"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1114..1116),aa:Thr,seq:tgt)
     gene            1511..2461
                     /gene="coaA"
                     /locus_tag="ACHKIR_004996"
     CDS             1511..2461
                     /gene="coaA"
                     /locus_tag="ACHKIR_004996"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFO9992600.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2490..3455)
                     /gene="birA"
                     /locus_tag="ACHKIR_004997"
     CDS             complement(2490..3455)
                     /gene="birA"
                     /locus_tag="ACHKIR_004997"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HFO9992601.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEKILSQLDDGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQHAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLTPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3452..4480)
                     /gene="murB"
                     /locus_tag="ACHKIR_004998"
     CDS             complement(3452..4480)
                     /gene="murB"
                     /locus_tag="ACHKIR_004998"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFO9992602.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAKGQSVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCIGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPSTVTPQQVFDAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETANALLAQFPTAPHYPQVDGSVKLAAGWLIDQCQLKG
                     TQIGGAAVHRQQALVLINEHDAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1182 a         1157 c         1045 g         1219 t
ORIGIN      
        1 gaatccagga agccagccag ttccaggatt ttcgcttccc actctgcgtc gccttccagc
       61 gctttcagag cagaaccacg aacgatcgga gtgtcgtcgc ccgggaagtc gtactgagac
      121 agaagttcac gaacttccat ttcaaccagt tccagcagct cttcgtcatc aaccatgtcg
      181 catttgttca ggaacacgat gatgtacgga acgcctacct gacgccccag caggatgtgc
      241 tcacgagtct gcggcatcgg gccgtcagtc gcagcaacta ccaggatcgc gccgtccatc
      301 tgagcagcac cggtgatcat gtttttaaca tagtcggcgt gccccgggca gtctacgtgc
      361 gcgtagtgac gggtcggggt gtcgtattca acgtgagaag tgttgatggt gataccacga
      421 gctttttctt ccggcgcgtt atcgatctgg tcgaatgcgc gagcagcacc gccgtaggtt
      481 ttagccagta cggtagtgat tgcagcggtc agcgttgttt taccatggtc aacgtggccg
      541 atagtaccga cgttaacgtg cggttttgta cgttcaaact tttctttaga catcgattgt
      601 ccctctaaga cacggataaa tcggtgatat caccacatca accaggcaac atgcccgact
      661 tgttgaatgc aataaacaga aggaaaaaac agggaggaga aaagaagtgg tgctgatagg
      721 cagattcgaa ctgccgacct cacccttacc aagggtgcgc tctaccaact gagctatatc
      781 agcacatctt ggagcgggca gcgggaatcg aacccgcatc atcagcttgg aaggctgagg
      841 taatagccat tatacgatgc ccgcatcctg gaactcggct acctgatttt cattctgcac
      901 tgaatatcga gagaagctct ctttattcga gccgataagc gaacttatcg tctcgggcta
      961 cgccatcgcg tggccgaaat tggtggtggg ggaaggattc gaaccttcga agtctgtgac
     1021 ggcagattta cagtctgctc cctttggccg ctcgggaacc ccaccggact tgatggtgcc
     1081 gactaccgga atcgaactgg tgacctactg attacaagtc agttgctcta cctactgagc
     1141 taagtcggca tcaagtagcg cgcattctat ggagacatgc gagttcatgc aactaaaaaa
     1201 ttgcataatt tgttttattg gtcacatttt atgcgacacg atgaagaaac agccgaacgg
     1261 agggcagatt agcacacttt ttcaacatca ttgtgctcaa caatgtgctc ctgctaaacc
     1321 ataattcttt ttatcagatg gaatatctgt cacattgctt ttcaacgata gcttcctggg
     1381 agagattttt tcttattatt cctccccatc tggtgttacc ctcctgccca ttaacccatt
     1441 caacagaact gtgacgcgcc atggcaaata tcgctttgcc gatagagcta tgaccgcctg
     1501 aaacatgctt atgagtataa aagagcaaac gttaatgacg ccttacctac agtttgaccg
     1561 caaccagtgg gcagctctgc gtgattccgt acctatgacg ttatcggaag atgagatcgc
     1621 ccgtctcaaa ggtattaatg aagatctctc gttagaagaa gttgccgaga tctatttacc
     1681 tctgtcacgt ttgctgaact tctatataag ctcgaatctg cgccgtcagg cagttctgga
     1741 acagtttctt ggtaccaacg ggcaacgcat tccttacatt atcagtattg ctggtagtgt
     1801 cgcggtgggg aaaagtacaa ccgcccgtgt attgcaggcg ctattaagcc gttggccgga
     1861 acatcgtcgt gttgaactga tcactacaga tggcttcctt caccctaatc aggttctgaa
     1921 agaacgtggt ctgatgaaga agaaaggctt cccggaatcg tatgatatgc atcgcctggt
     1981 gaagtttgtt tccgatctca aatccggcgt gccaaacgtt acagcccctg tttactcgca
     2041 tcttatttat gatgtgatcc cggatggaga taaaactgtt gttcagcctg atattttaat
     2101 tcttgaaggg ttaaatgtct tacagagcgg gatggattat ccacacgatc cacatcatgt
     2161 atttgtttct gattttgtcg atttttcgat atatgttgat gcaccggaag acttacttca
     2221 gacgtggtat atcaaccgtt ttctgaaatt ccgcgaaggg gcttttacag acccggattc
     2281 ctattttcat aactacgcga aattaactaa agaagaagct attaagactg ccatgacatt
     2341 gtggaaagag atcaactggc tgaacttaaa gcaaaatatt ctacctactc gcgagcgtgc
     2401 cagtttaatc ctgacgaaaa gtgctaatca tgcagtagaa gaggtcagac tacgcaaata
     2461 atttaaaggg gagttaatgc tcccctttct tatttttctg cactacgcag ggatatttca
     2521 ccgcccatcc agggttttat tattccatcc tgctcaagta ataaagcacc ctgtttgtct
     2581 attccgcgtg aaatgccaaa tatttcttta tcaccaatga taagtttcac tgggcgatta
     2641 ataaaattat ccaacttttc ccagcgcgaa agataaggtg tcaatccttc ttgctcgaag
     2701 agttccagcg ccgcgcgtaa ttcacgtatc agcatggccg caagagtatt acgatcgaga
     2761 ttgatcccgg cttcctgcag cgtgatccac ccttgattaa cgacactctc ttcaacacga
     2821 cgcattgcca tgttgatccc tgcaccaatg actatttgcg ccgcatcgcc ggttttgcca
     2881 gtcagctcca caagaatgcc tgccagcttg cgatcctgca gatagaggtc attaggccat
     2941 ttaacacgaa ctttatctgc acccagcttg cgtaatactt ccgccatcac gataccgata
     3001 accagactta aaccaatcgc cgccgccggg ccctgttcca gacgccagaa catcgacaaa
     3061 tataagttcg cgccaaaagg cgaaaaccat ttccgaccac gacgaccacg gccagcatgc
     3121 tggtattctg caacacaggc atcgccagat ttaagctctc cgatgcggtc caggagatac
     3181 tgattcgtgg agtcaataac tggcagcacg gctacactgc cgtcatccag ctgactcaat
     3241 atcttttcgg cattaagtaa ctggattggc tcgggcaagc tgtatccttt acccggaaca
     3301 gtaaatacat caacgcccca gtcacgcagt gtctgaatgt gtttattaat agccgcccgg
     3361 ctcattccca gcgtttcacc caactgctcg ccagagtgaa attcgccatt cgctaacagg
     3421 gcgatcaatt tcagtggcac ggtgttatcc ttcatgaaat tgtctccact gcgctcactt
     3481 cacctgatgc accaataaag cggacttcag gctcaagcca gacattaaat ttttcaccca
     3541 ctttttgtct tacatgatgc gccagctgta caacatcttc gctttttgca tcgtgctcat
     3601 ttatgagaac cagcgcctgt tgacggtgta ccgcagcccc accaatttgc gtccctttaa
     3661 gctggcactg atcaataagc caacctgcag ccagttttac tgaaccatcc acctgggggt
     3721 aatgtggggc tgttggaaat tgtgccaaca acgcgttagc agtttcggca gaaacaacag
     3781 gatttttgaa gaaactaccc gcattaccgt tcacttttgg atcagggagt ttggtggtgc
     3841 gcatatgaca caccgcatca aatacttgct gtggcgttac cgttgaagga tccaggcgag
     3901 ttaagtcacc atacgtcagt acaggttgcc actcttttgg cagacgcaga cctacggcta
     3961 caatggcgaa gcgatcctgg tattcatgtt taaaaatact gtctcgatag ccaaaacggc
     4021 actctttggc agttaagcgt acttgcttgc ctgtcgccag ttcaacacaa tcaacataag
     4081 cgcaaactcg ctgtaattct acgccataag caccaatatt ctggataggt gatgagccga
     4141 tacaaccagg aattaatgcc agattttcca gaccaggcat accttcctgc aaagtgtatt
     4201 ttaccaggcg atgccagttt tctccggctc ctacatgtaa ataccacgca tcaggttcat
     4261 catgaatttc gatacctttg atccggttga tgatcaccgt accgcgatag tcttccagaa
     4321 aaagtacatt acttccttca cccagaataa gaacggattg tccttttgcg gttgcatgct
     4381 gccaggcatt gagtagttgt tgttcgtctt cggcacatac aatgtgctga gcattatgat
     4441 caatgccaaa tgtgttccag ggttttaagg agtggttcat agctgctttc ctgatgcaaa
     4501 aacgaggcta gtttaccgta tctgcggggg gatggcttgt agatatgatg acaggaagag
     4561 tttgtagaaa cgcaaaaagg cccagtcttc cgactgggcc ttc
//
LOCUS       DAZGQL010000195         4569 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.197, whole genome shotgun sequence.
ACCESSION   DAZGQL010000195 DAZGQL010000000
VERSION     DAZGQL010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4569
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.197"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(99..386)
                     /locus_tag="ACHKIR_004999"
     CDS             complement(99..386)
                     /locus_tag="ACHKIR_004999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HFO9992603.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLFIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTVESQKSVEDEDIIRLVAER"
     gene            complement(461..604)
                     /gene="mgrB"
                     /locus_tag="ACHKIR_005000"
     CDS             complement(461..604)
                     /gene="mgrB"
                     /locus_tag="ACHKIR_005000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HFO9992604.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            763..1002
                     /locus_tag="ACHKIR_005001"
     CDS             763..1002
                     /locus_tag="ACHKIR_005001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobH family protein"
                     /protein_id="HFO9992605.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            complement(1146..1937)
                     /gene="kdgR"
                     /locus_tag="ACHKIR_005002"
     CDS             complement(1146..1937)
                     /gene="kdgR"
                     /locus_tag="ACHKIR_005002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HFO9992606.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            2114..3487
                     /locus_tag="ACHKIR_005003"
     CDS             2114..3487
                     /locus_tag="ACHKIR_005003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9992607.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIGAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLIAAAILAVIAACVSGLRI
                     TQPRSRA"
     gene            complement(3533..4414)
                     /gene="htpX"
                     /locus_tag="ACHKIR_005004"
     CDS             complement(3533..4414)
                     /gene="htpX"
                     /locus_tag="ACHKIR_005004"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HFO9992608.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
BASE COUNT         1118 a         1173 c         1022 g         1256 t
ORIGIN      
        1 agagttttaa tttgacgcag ttgcaccatg acagtgcgta taatcaggat gaattgagta
       61 acccttcaac gacggggtta ctcaagattg aggaaagatt atcgttcggc gaccagacga
      121 ataatatctt cgtcttcaac cgatttttga ctctcaacgg tctttttatc tgctttctct
      181 ggttcgtttg cttcgcacaa acgacgtaac agagcatttt gacgtttttg ctgatccagc
      241 aacgcctcaa gcagttcaat ctgttcgtta gtccgtgaac tggcacgatt gataaaaaac
      301 cacaagataa gcccgataaa aagaaccacc accgatacaa ccaaagacgc aatgttcatc
      361 acgcctgaat ttacaacttc gttcatttca ccacctctga gtagaggcgc tattctacca
      421 ctgctggaga ggaagaaaat ctagtgctga aaaaatgata tcaccacggg ataaactggt
      481 taatggcaca aattccgctg aaaaattgta catcctgatc gcacatcatg ttgaatacct
      541 gcgcccaaag cagcaagcaa gccaacacca cgacaaccag aacgacccat cgaaactttt
      601 tcactccaat ctccgtttca cctaccttat gtcattaaac tagcatgcgt tagttaaaca
      661 gcgcctaact atgtcgatat tcgtgctttt tcagaatgag tcgcttgctt cagcgcatat
      721 tgccgctacg attaagcgaa cataaaaaga gaagaggttg taatgcgatt catcattcgc
      781 acagttatgc tgatagcact ggtatggata ggattattac tcagcggata tggcgttttg
      841 attggaagca aagagaatgc cgcgggatta ggtttgcaat gtacctatct gaccgccaga
      901 ggcaccagca cggtacaata cctgcacact aaaagtggtt ttctggggat aacagattgc
      961 ccgttgttac gtaaaagcaa tattgtggtc gataatggtt aagggtaata aataaccaga
     1021 ttctcactca tgagtaaaat attcttatcg caagtgcgtt taatataacg ccaccttcaa
     1081 ctttattttt ctcatgctaa ataaagaacc tgtctgtttg atattcagac aggttcttac
     1141 tctcatcaga acggatagtc gtgataaccc atttgggcag aaattttgcg cgccgcggta
     1201 tgcaacattg cgacatattc ctgtaaacgc tcttcagaga aacgtaacgt cgggaaggag
     1261 atgctcaaac cggcaatgac cacgccaaag cgatcaaata ccggaaccgc aatgcatcgc
     1321 agcccttctt cctgctcttc gttatcttcg ccatacccct gctcgcgcac ctggtccaga
     1381 acgggtaata acgcttctgt actggtgatg gtccgctcgg tactgcgttt atactccacg
     1441 ccctcaagaa tttgcttcac ttcatcgcga tcgcgccatg ccagcagtac cttaccaatc
     1501 gcggtgctgt acagcggatt acgacgccca atccgtgaat acatgcgcaa attgtacata
     1561 gagtcaattt tgtgaatgta aacaatactg tcttcgtcca gtgcgccgag gtggatagtt
     1621 tctttggtca ggcgggagat ctcacgcatc tggatatctg cgctacgaat taaatcgacg
     1681 ttttgtaacg cgcgagcgcc cagttcaaac aatttcaggg tcagcgaata tttctccgac
     1741 tccccttcct gcgccacata acctaaggtt ttcatggtct gtaaaaagcg ataaacggtg
     1801 ctttttgaca tcatgacgcg ctgcgacaac tcggttatcc ctatttcgcg ctcttcaccc
     1861 agcgcctgca aaatgccaaa aacttttagc acggaagata cagaatcagg ctgtttatcc
     1921 agatctgcgt tagccattta tcacctcatt gcgagtgttt tataaaaatc agaactgttt
     1981 tttattataa tttcgcacca gggtggtcgc aatccatctt ttgccggtta gttacaattc
     2041 tgcgacatcc accgtgaata tcagtgctag aatcataccc ctgttgatta ttcaccaaag
     2101 atataaaatt cctatgccaa aagttcaggc cgacggcctg ccattgcccc agcgatacgg
     2161 tgcgatatta accattgtga ttggtatttc gatggctgtc cttgacggcg caatcgccaa
     2221 cgtcgccttg ccaacaatcg ccacggacct tcatgccacg ccagccagtt ccatctgggt
     2281 agtgaacgcc tatcaaatcg ccattgtcat ctccctgctc tcgttttcgt ttctgggcga
     2341 tatgtttggc tatcgacgta tttataaatg cggtctggtc gtttttctgt tgtcttcact
     2401 attctgcgcc ctttctgatt cgctgcaaat gctcaccctt gcgcgtgtca tacaaggttt
     2461 cggcggtgca gcgttgatga gcgttaatac cgcacttatc cgcctgatct atccacaacg
     2521 ttttctgggt agagggatgg gcataaactc gtttattgtt gccgtttctt ctgctgccgg
     2581 gccgacaatt gctgcagcaa tcctctccat cgcatcctgg aaatggttat ttttaatcaa
     2641 cgtaccgttg ggtattatcg ccctgcttct ggcgatgcgt tttctgccac ccaatggttc
     2701 tcgcgccagt aaaccccgtt tcgacctgcc cagcgccgtg atgaacgcgt taaccttcgg
     2761 cctgcttatt actgcattga gtggtttcgc tcaggggcaa tcgctgacat tgattggtgc
     2821 ggaactggtg gtaatggttg tcgttggtat tttctttatt cgccgccagc tttctcttcc
     2881 cgtaccgctg ctaccggtgg atttactgcg tatcccgctg ttttcacttt ctatttgcac
     2941 atctgtttgc tctttctgcg cacaaatgct ggcaatggtt tcccttccct tttacctgca
     3001 aaccgtgctc gggcgtagtg aagtcgaaac aggtttactt ctgacaccgt ggccgttagc
     3061 aacaatggtg atggctccac tggcaggcta tttgattgaa cgcgtacatg caggattgct
     3121 gggtgcttta gggttattca tcatggctgc ggggcttttt tccctagttc tgctgccagc
     3181 gtcacctgcg gatatcaata ttatctggcc gatgatctta tgtggtgctg gatttggctt
     3241 gttccagtca cccaataacc acaccattat tacctccgct cctcgcgaac gtagcggtgg
     3301 agccagtggc atgttaggga cggctcgtct tctgggtcag agtagcggcg cggctctggt
     3361 ggcgctgatg ctaaatcagt ttggtgataa tggtacgcac gtctcgctga tagctgcggc
     3421 tattctggcg gtgattgcag cctgtgtcag tggtttacgt atcactcagc cacgatccag
     3481 ggcataaaaa aagcgcgtcg atcaggacgc gctttttagt atttacttca tattacttca
     3541 ggtattcacc cgtacgcaga gcttcaatac gtttatccag tggcgggtga gtcatgaaca
     3601 actcactgag cgatttcgac ttcccgttaa tgcagaaagc catcatgctg gttgcttctt
     3661 gcggttcata gctggttttc aggcgctgca atgcagcaat cattttctcg cgaccaacca
     3721 gttttgctga acccgcatcg gcgtggaatt cacgatgacg cgagaaccac atggtgataa
     3781 tgctcgccag aataccaaac accagttcca gaaccgttgc aaccgcaaag tagatcagcg
     3841 ggttgccgtt gctctcttca ccttcatcac gatttccgcc cataaaaccc gcggcaagct
     3901 gcgccagaat acgggaaata aagataacga aggtgttcac cacgccctga atcagcgtca
     3961 tggtgaccat atcaccattg gcgatgtggc tgatttcgtg agcaattacc gcctcggctt
     4021 catccgggct catgttctgc agcaaaccgg tgctgacagc aaccagagag gcatcacggc
     4081 gcgcaccggt tgcaaaagca ttgatgtccg gcgcatggta gatagccact tgcggcatag
     4141 cgatccccgc ctgacgagcc tgggttgcta ccgtattgac cagccaacgt tccctttcgt
     4201 tacgcggttg ctcgatcact tccccgccaa cagatcgtaa tgccatccat ttggacatca
     4261 gaagcgaaac gaaggaacca ccaaaaccga acagcaaggc catgatcatc agcccctgaa
     4321 cgctgctcga ctgtatccct gtcaggctca gtaccagccc gaaaacgacc attacggcca
     4381 ggttcgttag caggaagagc gcgattcgca tcataatttt ctttttacct cagtttaaca
     4441 aaacgcaata tgcgataccc acatcgtatg ggttacgcga ctattttcaa gtctggatag
     4501 tgcgtaagtc accagaaaga cacaacttta cattttgtag catctgattt acggcatctt
     4561 gctgctgtt
//
LOCUS       DAZGQL010000196         4550 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.198, whole genome shotgun sequence.
ACCESSION   DAZGQL010000196 DAZGQL010000000
VERSION     DAZGQL010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4550
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.198"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..1104
                     /locus_tag="ACHKIR_005005"
     CDS             <1..1104
                     /locus_tag="ACHKIR_005005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2345 domain-containing protein"
                     /protein_id="HFO9992609.1"
                     /translation="RQSRPYAGDTYGLHLPLLAGTEVSIAFEEGNPDRPYIAGVKHDS
                     AHTDHVTIQNYKRNVLRTPANNKIRLDDERGKEHIKVSTEYGGKSQLNLGHLVDAGKQ
                     QRGEGFELRTDMWGAVRAKKGIFISADAQDKAQGQVREMADIISELNGLSDKIQKLSD
                     DATTANVDPADMAAQIALITSRINDLTASVILMHAPKGVAVASGEHLQLAAVKNLQIN
                     AGNNADIGVVKNMFIGVGRALSVFVRKAGIKLIANKGAVSVQAQHDLMELLAKKSIEI
                     VSTEDEIRISAKKKITINGGGSYIRIDSGGIEPGTPGDYNVKAVHYGRQPKASEKVPM
                     PEFPILSAVDSSDFCLECLLNAIKNDDAVVEGA"
     gene            1107..1928
                     /locus_tag="ACHKIR_005006"
     CDS             1107..1928
                     /locus_tag="ACHKIR_005006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4123 domain-containing protein"
                     /protein_id="HFO9992610.1"
                     /translation="MTSEQQLADHWLKRNETLNAFILFEGGALSQEMLQIMMEHYKYR
                     RISLYQLPDLKKVAAYGPWLVRIESEKDLAMVLVGKLPVAGITFSELQLEPLASRLAV
                     GCMAKTPDGREVLLRYYMPRVLKQLAARPDMEWHPVMFSQIESWWMLEDTRWQRLNMP
                     LTAANNSDETVEIITLDEPLWQKIADREDVSALLREWRTMKTSEHFPPCIQRNMVEKA
                     LNKAQAVGLTKPLDQKIYALSYLNGKKAYLESAEFQVMLEKVKRNEISLSDLGRQ"
     gene            1972..2526
                     /locus_tag="ACHKIR_005007"
     CDS             1972..2526
                     /locus_tag="ACHKIR_005007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3304 domain-containing protein"
                     /protein_id="HFO9992611.1"
                     /translation="MGLFTRLEKFDQKLTRGYARWGRWVWRMLIAVPVLVVLWNIGQA
                     VWGGPRGGVILEIHSEIDRPILGFSVNGVAGANAFANGGGSVTCCGDVSGDTAEVIWT
                     LSTTRTQYNAGMRLEKRNKNLPLPKREWGENYLHVHFMPGDKVLLGWSKDSFSPYEDL
                     HNGGYKTRVRQDVKDKLYGTGKMN"
     gene            2567..4516
                     /locus_tag="ACHKIR_005008"
     CDS             2567..4516
                     /locus_tag="ACHKIR_005008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2235 domain-containing protein"
                     /protein_id="HFO9992612.1"
                     /translation="MWGMLDTGGGIRAQLAEDNKVGNCGREWHVGFFFDGVGRNIDQD
                     ASEQRLSNVARLFRAYPDEDSNTDFVCHNKFYFSGMGTPYNEELISKLYTIMDQSLES
                     MKEDLNDFAKETHEEISKEIRNRRGSWNETLKKIQDKLLDPKELRKKRNDIFKNMAKK
                     SIVESTQWIRDNEIISAFFLTGEVTRINAAKVLFANAFVENSTNKNDSTAIKIKSISV
                     SLYGYDTGAALARKFLDELLEEFCEKEGEDKYLFKKVPVNIVFAGFFDCSRHSPASNN
                     NGLDYFLSLPGEITKNNKLKTAGKIAKVAFGEKAIELDTILPGTVKNALHLVAAYERR
                     LWRSLYQLGGMNAEHKEILLPGCSEDVGGGLKPDEQKPSAELCRVALQKMYEAAYDAG
                     VPYTDFSVLDENDSKVSRYFLMNDAVEGKSVKEWMKSYEMEVGQCQKETQSASESKVN
                     DTDNKNDLPFDFYLDIYFKWLANQYYLYCTELYQLDEKLSLAHRKQISGHGPLAGTRI
                     NPNPEADEINAQIAELKSHWGWLDDVRRVATGLSNDFNYGRPMDTRMLNHEDIFRPAW
                     KRAELFLDYYHKAWNGEELTEISWLGIDTIHSYFTHDLQTVDTGASINESFFLRRMAE
                     YPKAKEKPEEKSEEQSPSPDLSGDD"
BASE COUNT         1341 a          896 c         1220 g         1093 t
ORIGIN      
        1 cgccagtcgc gcccgtatgc gggagacacc tacggcctgc acctgccgct gctggcagga
       61 acggaagtca gtatcgcctt tgaggaaggc aacccggacc gaccttatat tgcaggcgta
      121 aaacacgact cggcgcacac cgaccatgtg accattcaga actacaagcg caacgtactg
      181 cgtaccccgg cgaacaacaa aatccgcctc gacgatgagc gcgggaaaga gcatatcaag
      241 gtcagcaccg aatatggcgg caagagccag ctaaacctcg gtcatctggt ggatgccggg
      301 aaacagcagc gcggggaagg ctttgaactg cgtaccgata tgtggggcgc ggtgcgggcg
      361 aagaaaggga tttttatcag cgccgatgcg caggacaagg cgcagggtca ggtcagagag
      421 atggccgaca ttatcagcga actgaacggt ctgagcgata aaatccagaa actgtccgat
      481 gacgcgacga cggcgaacgt cgacccggcg gatatggccg cgcagatagc attgataacc
      541 agccggataa acgacctgac cgcctcggtg attctgatgc acgcgccgaa aggtgtggcg
      601 gtcgccagcg gcgagcacct gcaactggcc gcagtgaaaa acctccagat aaatgcggga
      661 aacaacgccg atatcggcgt ggtgaagaat atgtttatcg gcgtggggcg ggcgctgagc
      721 gtgtttgtgc gcaaggcggg tatcaagctg attgccaata aaggcgcagt atcggtacag
      781 gcgcagcacg atttgatgga gttactggcg aagaagtcga tagagatcgt cagtaccgaa
      841 gatgagatca ggatttcggc gaagaaaaaa atcacgatta atggcggcgg gagttatata
      901 cggattgata gcggcggcat tgagccggga acgccggggg attataacgt caaagcggtg
      961 cattacgggc gccagccgaa agcctcggag aaggttccta tgccagaatt tcctatcctt
     1021 tcagccgtgg actcatccga tttttgtctt gaatgcctgc tgaatgccat caaaaacgat
     1081 gatgctgttg ttgaaggagc gtaatcatga cttcagaaca acaactggca gaccactggc
     1141 ttaagcgaaa cgaaacgtta aacgccttta ttttatttga aggtggagcg ctctctcagg
     1201 aaatgcttca gatcatgatg gagcattata aataccgacg aatttccctg tatcaactgc
     1261 ccgatcttaa aaaggtagct gcttacggtc cctggttggt tcgtattgaa agtgaaaagg
     1321 atctggcgat ggtgcttgtc gggaaactac ctgtcgcagg cattactttc tctgaattgc
     1381 agctggagcc actggcgtcc cggcttgctg tgggatgtat ggcaaaaacg ccggatggtc
     1441 gtgaagttct gctgaggtac tacatgcccc gagtgctgaa acaactggca gcccggcctg
     1501 atatggagtg gcatccggtg atgttctctc agatagaatc atggtggatg ctggaagata
     1561 cgcggtggca aaggctgaac atgccgctta ctgcggcaaa taacagcgat gagacagtgg
     1621 aaattataac gcttgatgaa ccgctctggc agaaaatagc ggatcgggaa gatgtatctg
     1681 cgcttttaag agagtggcgc accatgaaaa cgagtgagca cttcccgccc tgtattcagc
     1741 gaaatatggt ggagaaggca ttgaataagg ctcaggcggt cggattgaca aagccactgg
     1801 atcagaaaat ttatgcgttg agctatctga atggaaagaa agcgtatctt gagtcagccg
     1861 aatttcaggt catgctggaa aaggtgaaac gaaatgaaat atcgctgtct gaccttggtc
     1921 gccagtgaca tacatctttg ccaaccaatt gataaaaggg aataaaatct gatggggcta
     1981 tttacccgac tggaaaaatt tgaccaaaag cttacgcgtg gttatgcccg ctgggggcgt
     2041 tgggtgtggc ggatgctgat cgccgtgcca gtactggtcg tattgtggaa tatagggcag
     2101 gcagtatggg gaggtccaag aggtggagtg attctggaaa tccacagtga aattgaccgt
     2161 ccgatactgg gattcagtgt taacggcgtg gcgggcgcga atgcttttgc aaatggtggc
     2221 ggttcagtca cctgttgcgg tgatgtcagt ggggatacgg cagaggttat ctggacatta
     2281 agtactactc gtacacagta taatgctgga atgcgtctgg agaaacgtaa caagaatttg
     2341 cctctcccaa aaagagaatg gggagaaaat tatctgcacg tgcattttat gcctggcgat
     2401 aaagtgttgc tggggtggag taaagattct ttttctccgt atgaagatct tcacaacggg
     2461 gggtacaaga cccgcgttcg gcaggatgtg aaagataaac tttatggcac agggaaaatg
     2521 aattgataga tttctgtaaa aatcacgttc agcaggaggc gtaataatgt ggggaatgtt
     2581 agataccggt ggtggaattc gggcacaact cgcggaagac aacaaagtag gaaactgtgg
     2641 gcgggagtgg cacgttggtt ttttctttga tggcgtgggg cgcaatattg atcaggatgc
     2701 ttcagaacaa cgcttaagta atgtggctcg tttatttcga gcttatcctg atgaagattc
     2761 caatactgat tttgtttgtc acaataaatt ttatttttcc ggtatgggaa caccctataa
     2821 cgaagaattg atctcaaaat tatatacaat catggatcag tctctggaaa gtatgaagga
     2881 ggatttaaac gatttcgcaa aagagacaca tgaagaaatc agcaaggaaa tccgtaacag
     2941 aaggggatcg tggaatgaaa ccttaaagaa aatccaggat aaacttctgg accctaaaga
     3001 acttagaaaa aaaagaaatg atatctttaa aaacatggct aaaaaatcca ttgttgagtc
     3061 aacgcaatgg atccgcgaca atgaaattat tagtgctttc ttcttaacgg gggaggtcac
     3121 gagaattaat gcagcaaagg tgttgtttgc gaatgctttt gttgaaaaca gcacgaataa
     3181 aaatgattca actgcgatta aaataaaatc aatatctgtt tcgttgtatg gctatgacac
     3241 cggagctgca ctggcacgaa aatttctgga tgagttactc gaagagtttt gtgaaaaaga
     3301 aggtgaggat aaatacctgt ttaaaaaggt accagtaaat atcgtctttg ccggcttctt
     3361 tgactgttca cgtcattcac cagccagtaa taataatggg ctggactatt ttttaagtct
     3421 tccgggggaa attaccaaga ataataagct gaaaacagct ggcaaaatcg ccaaagtcgc
     3481 atttggcgag aaggccatcg aactggatac catcttaccc ggtacagtta agaacgcgtt
     3541 acatctggta gcggcttatg agcgacgtct atggcgcagt ctttaccagt tggggggtat
     3601 gaatgcggaa cataaggaaa ttttactgcc tggttgtagc gaggatgtgg gcggtggtct
     3661 gaagccagat gaacagaaac caagcgcaga gttatgtcgt gtggccctgc aaaagatgta
     3721 tgaagcggca tatgatgctg gggtacctta tactgatttc tctgttctgg atgagaacga
     3781 tagtaaagtg tctcgctatt tcctcatgaa tgatgctgta gaaggcaaat ccgttaaaga
     3841 gtggatgaaa agctatgaaa tggaagtggg acagtgccaa aaagaaacgc aaagcgcctc
     3901 tgaaagtaaa gtaaatgata cggataataa aaatgacctg ccctttgatt tttatcttga
     3961 tatatatttc aaatggttgg caaatcaata ctatctttac tgtactgaac tttatcaatt
     4021 ggatgaaaaa ctatcacttg cacatcgcaa acaaataagc gggcatggcc ctcttgccgg
     4081 aacgagaatt aaccctaatc ctgaagctga tgaaatcaat gcacagatcg cagaacttaa
     4141 atctcactgg ggatggctgg atgatgtaag gcgtgtcgct acaggattat cgaatgattt
     4201 taattacgga aggccaatgg atacccgcat gctaaatcat gaagatattt ttcgcccggc
     4261 atggaagcga gctgaactgt ttcttgacta ttatcataaa gcatggaacg gagaagaact
     4321 cacggagata tcatggctag gcatagatac tattcattct tattttactc atgatttaca
     4381 aactgttgat accggggctt ctattaacga aagttttttc cttcgtcgta tggctgaata
     4441 tcccaaagca aaagaaaaac cagaagaaaa gtcagaagaa cagtcaccat ctccagattt
     4501 gtcaggtgat gattaaatca ggtgatgtct ctctacaata aaataaattt
//
LOCUS       DAZGQL010000197         4507 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.199, whole genome shotgun sequence.
ACCESSION   DAZGQL010000197 DAZGQL010000000
VERSION     DAZGQL010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4507
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.199"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            180..647
                     /locus_tag="ACHKIR_005009"
     CDS             180..647
                     /locus_tag="ACHKIR_005009"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992613.1"
                     /translation="MKGKIIPEKNKIKNLINQERDAIALWIKDGMRLPDIYRLLKEKH
                     PELTFSQNGFVFSLRRDASDLHENALFNRSTVAVFMQKQHSEMTLMVNSGCLLKNVHE
                     ALFSHISYSVFLRYIVKLYPDLHYQAKLNRKGAVCGELHHEENASVYQNDEVC"
     gene            complement(770..1051)
                     /locus_tag="ACHKIR_005010"
     CDS             complement(770..1051)
                     /locus_tag="ACHKIR_005010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992614.1"
                     /translation="MKTNKKGTTWHIFYRENSGAEVLLEIPSFRECLSVSKELMTPSN
                     YMICIEKNGERIKRWDREIIAGSNKWINCPPDNFEILGELITINRIIKK"
     gene            2119..2346
                     /locus_tag="ACHKIR_005011"
     CDS             2119..2346
                     /locus_tag="ACHKIR_005011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HFO9992615.1"
                     /translation="MFPEYRELISALKGNHKRFDSLFEKHNKLDHEIKRIEDEFGSAY
                     STKVVRLKLEKLEIKREIQKILEDESEKGER"
     gene            2668..3243
                     /gene="dolP"
                     /locus_tag="ACHKIR_005012"
     CDS             2668..3243
                     /gene="dolP"
                     /locus_tag="ACHKIR_005012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001238641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HFO9992616.1"
                     /translation="MRLLYSAVVLTSALLLQGCVAAAVVGTAAVGTKAATDPRSVGAQ
                     VDDEVLELRVSSALSKDEQIRKEARISEIAYQGRVLLIGQSPGRELSARARQIALGVD
                     GVQEVYNEIRQGTPVSPVIMAGDAWLTTKIRSQLLTSARVKSSSVKVVTENSEVFLLG
                     LVTEQEGRAAADVASRVSGVKRVTTAFSYIR"
     gene            3661..3942
                     /locus_tag="ACHKIR_005013"
     CDS             3661..3942
                     /locus_tag="ACHKIR_005013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFO9992617.1"
                     /translation="MPSATQTARLEARISTELHAMLKRAAEIQGRTMTDFIIAAVQDA
                     AQRAIEQTDIIRLSRTDQEIFAQALLSPTEPNDALKRAFKRRNNMLYTK"
     gene            3948..4436
                     /locus_tag="ACHKIR_005014"
     CDS             3948..4436
                     /locus_tag="ACHKIR_005014"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO9992618.1"
                     /translation="MFQLTPFNSTYDRTSFLCGSDALDRYIKEHVTQDIRRRIAACFV
                     AVDNDHHIIGYYTLSAASILLTDLPEHLRKKLPRYPTIPAIRMGRLAVSLSSKGTGLG
                     GALLADALYRAYTSEIAAYALIVDAKDDTAVAFYKHYGFMAFPESGRTLFMPLASLST
                     IK"
BASE COUNT         1273 a          878 c         1023 g         1333 t
ORIGIN      
        1 tcatggcagc ctcatgtatt tatgtggaga ttatttcatc ggatattatt ttatccagga
       61 atgttgtttt gttaaagtga gtaagtctta tagattatgt ttctttactg aattccgggt
      121 atgtagacaa agctggttct gtatatgtga acttctttgt tgttcctgag ggagataata
      181 tgaaaggcaa gattatacca gaaaaaaata agataaaaaa cttaatcaac caggagcgtg
      241 acgccattgc actctggatt aaggatggaa tgagactgcc agatatttac agattactga
      301 aggaaaaaca cccggaactg acgttttcac agaatggttt tgtattttca ctgagacgtg
      361 atgcaagtga tttacatgaa aatgcgcttt ttaatcgcag tactgttgct gtatttatgc
      421 agaaacaaca ttctgagatg actctcatgg ttaattctgg ttgtttgctg aaaaatgtgc
      481 atgaggcgtt attcagtcat atatcttact ccgtgtttct tcgatatatt gtgaagttat
      541 atccagattt acactatcag gcaaaattaa acaggaaagg ggctgtctgt ggtgaattac
      601 atcatgagga gaatgcttct gtttatcaga atgacgaggt ttgttaaagg tgttttataa
      661 aaatagtggg gtagtgctgg cacagagatg tcttacaggc attctcccgc aaaaaatgaa
      721 cccgttgctt gttatagtgc cttgcttaat acattgcctg aatccggagt tactttttaa
      781 taattctgtt aatggttatt agctcaccta atatttcgaa gttatcaggt gggcagttaa
      841 tccatttgtt agagccagcg atgatttccc tgtcccagcg ttttattctt tcgccatttt
      901 tttctatgca tatcatgtaa tttgatgggg tcattaattc tttggatact gaaagacatt
      961 cccgaaatga tggtatttca agcaaaactt ccgcgcctga attttcacgg taaaaaatgt
     1021 gccatgttgt gcctttttta ttagttttca cgtgcgtatt ctcataggat aaatataaaa
     1081 ccttgtaaca ttcaggaggt aacaggattt attctgtaag aacaacagac agaacaatga
     1141 tatctgtttt atgtatacga tgtagccagt gatttagtac attgctggct atatcacttt
     1201 tcccattttg tgataagtct gctttgaaac gatggtgtcc ctcctcctgt attgtgaaag
     1261 ttgccacatt tttttcttca tatccctgaa catccccgtc gtgccttata taattgactc
     1321 cattaagaag ttgtcagaat aattatttct gattgcaacg gagttaattt cagtgatggt
     1381 aaagatatcc gtggcaccat aacgcatttt tcatcatcag tatagtggta tgcgtgaaca
     1441 tatatggtgg cattttgcct gatgttgtta aggcttttca gcaggtctac agtcaactcc
     1501 atcgaggtat ccttcaggtt gttatcattc gttgatgatg cgatctgttg aaggcgttgg
     1561 cgacaattaa ttctttttct cttctgaaaa atttacggaa tacaaaaaag atctgttttt
     1621 aactgattgt tactttctga agatattctc tgctccattt gctttccggg taagacgtat
     1681 ggctggttat ggtatggtcg gactgcagcc acagggggat gactgtaata cctctgatca
     1741 ccacagtcag tcatctgtaa atgttaaata cctcacagtt cagtcagtgg attattgtcg
     1801 gtcttacggg atatttgtta aaaaaatact gcatggtgaa tccccctgag cggcggggca
     1861 tatcagtatc gtaggtgttt ggtcacatcc cttatctgtg cgggttcagg tgctgatact
     1921 gaactcaccg ggaggcaccc ggcaccatgc tacataacac tccattacgc tacactcgcc
     1981 cctctccgga ggggttttct ctgcaggatg tgaatatgag atgctgcatc attcactgcg
     2041 aatattgatg gtgagaatct ttaataatat taatgtttat atgtattgtt acagtgttga
     2101 tgaaaggaga taaaaacaat gttcccggag tacagagaac tgatttcagc attgaaagga
     2161 aatcataaac gctttgattc tttatttgaa aagcataata aacttgatca cgaaataaaa
     2221 agaattgaag atgaatttgg ttcagcgtat tcgacgaaag tggtcaggct gaagcttgaa
     2281 aaacttgaga tcaaaagaga aatccagaaa atccttgaag atgagtcaga aaagggcgaa
     2341 cggtaatcat aaatggtata ttcaaagacg tgttttctct gttttatgaa aaaggcaccg
     2401 ggggctccac ggtgcctttt agcctgtgta taacgaaaag gagatcctgt tcagcgaacc
     2461 agatagttaa aaaaagaatc tttacgacgg tatcaaagtt ttcgtgcaaa aaacagatac
     2521 agatatgcca cgtaacgcat ccgctatttc cggctgtgcc ggaggataat aacagtctgt
     2581 agcttgtttt gtttcactga aggtaaactt tttgtacgat aaattccggg aatatgaagt
     2641 aaaaatcaaa acaattccgg gggagatatg cgtttattat attctgctgt tgtccttact
     2701 tcagccttat tgttacaggg atgcgtggct gccgctgtgg tgggtacagc tgctgtcggg
     2761 acaaaggcag caaccgaccc acgcagtgtc ggagcgcagg tggatgatga agtgctggag
     2821 ctgagggtca gcagtgcact ttcaaaagat gaacaaatca gaaaagaagc acgcatcagt
     2881 gagattgcat atcagggacg cgtgctgctg atcggtcagt ctccgggccg tgaattatct
     2941 gcccgtgccc gtcagatagc actcggtgtc gatggtgttc aggaggtcta taatgaaatc
     3001 cggcagggga cacccgtcag tccggtcata atggcaggtg atgcctggct tacgacaaaa
     3061 attcgttctc agttactcac aagtgcccgt gtcaaatcat ccagtgtcaa ggtggtgaca
     3121 gaaaacagcg aagtcttttt actgggcctg gttacagagc aggaagggcg agcggcagca
     3181 gatgttgcca gtagggtgag cggagtgaag cgggtcacga cggccttctc ctatatccgg
     3241 tgaaggcagt atcagagttg cctttagtgt aaatttacgt taaaaatcag aagagcacgt
     3301 ttccgggccg ttcatgccac cacgtgaaca ttcgtcgcgg aataccggag gcgtgctcat
     3361 tataataacc aatatacata attcctcttt gacacccccc tgaattcggg ggattttttt
     3421 gcccgggtat gttcactcac agataaaatc ccggtgtact ccggatgtca ctgctggtaa
     3481 ataatctgag cttttatctg tatgcaatgg tcaaagatcg tggcgttaac atcccccgga
     3541 tagttttgtc tgaatctgat ttttcttttc aggcagaatg tctgtctgcc gtcccggtgt
     3601 cagagtgtaa tttgcacaat acggcaattt gccgtacagt gtattcagga ggtacaaatc
     3661 atgccatctg caacacaaac tgcccgactg gaagcaagga tcagcacaga actacatgcc
     3721 atgctgaagc gcgctgcaga aatccagggg cgcactatga ccgattttat cattgctgcc
     3781 gttcaggatg cggcccaacg cgctatcgaa caaaccgata taatccgact gtcccggaca
     3841 gatcaggaaa tttttgctca ggcattactg tcaccaacag agccgaatga tgcgcttaaa
     3901 cgggccttta aacgccgtaa taatatgctg tacacaaaat aaatataatg tttcaactga
     3961 caccatttaa cagcacatat gacagaacct catttttatg tggctctgat gctctggacc
     4021 gttacataaa agagcatgtc acacaggata ttcgacgacg catcgcggcc tgttttgtcg
     4081 ctgtagataa cgatcatcat atcatcggat actatacgtt atcagcggcc agtatattat
     4141 taactgactt acctgagcat ttgagaaaaa agcttccgcg atatcccact attccggcta
     4201 ttcgtatggg acgactggca gtaagtctgt catcaaaagg aacggggctt gggggagcac
     4261 ttttagctga tgcgctatat cgtgcataca catctgaaat tgcagcatat gcgcttattg
     4321 ttgatgcaaa agatgacacg gcagtcgcat tttacaaaca ttatggcttt atggcgttcc
     4381 cggagtccgg aagaacttta ttcatgccat tggcatcatt atccacaata aaataaacag
     4441 aataaatcag ggaaatacgg tgcttgtggc aacgtcagcc cacgaagtta aatgttaacg
     4501 ggcaaca
//
LOCUS       DAZGQL010000198         4459 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.200, whole genome shotgun sequence.
ACCESSION   DAZGQL010000198 DAZGQL010000000
VERSION     DAZGQL010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4459
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.200"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..408)
                     /locus_tag="ACHKIR_005015"
     CDS             complement(<1..408)
                     /locus_tag="ACHKIR_005015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFO9992619.1"
                     /translation="MNGIKTGLGITPGEHIISADSALSRNIRQCFCLSCRGRLILQTD
                     AQGAWFEHDSHALSAQQKATCVVLNPEKSHPYIEDMAMFLSPLPVVLEWHCVMCEQFF
                     HGKKFCEACGTGIYCRATCTKSVYRYQPDLCQDC"
     gene            complement(405..788)
                     /locus_tag="ACHKIR_005016"
     CDS             complement(405..788)
                     /locus_tag="ACHKIR_005016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFO9992620.1"
                     /translation="MYAKSFIAFDGNGRLTGARTAQTAPYDRYTCHLCGSALRYHPQY
                     DTERPWFEHADEGLTEHAQQCPYVRPERREIRLIKRLQQFVPDALPVVRKASWRCRQC
                     HHDYYGERYCTHCHTGHFSEEGVAE"
     gene            complement(1160..1753)
                     /locus_tag="ACHKIR_005017"
     CDS             complement(1160..1753)
                     /locus_tag="ACHKIR_005017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFO9992621.1"
                     /translation="MYNANPNYEMNFAILKDVNEHMDGMFQRFSKLLPFRIDFAYRKD
                     TPSFGHSCKHSMCMEIYRLLSETQTMLAGYYWVMEYTPDKGLHIHFIGYLDGQRHKNS
                     YQISRQLGDIWRRITEGEGYFHLCRAKDKYPVRIDHVIHYSDKSAVDDLRYALSYLAK
                     QDQKEHGIILGRSRLPEKSNRGRPRLDSTLSEICSLV"
     gene            complement(2985..>4459)
                     /locus_tag="ACHKIR_005018"
     CDS             complement(2985..>4459)
                     /locus_tag="ACHKIR_005018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001242275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992622.1"
                     /translation="GFYYHIYNFLQKSYCPCLDYSGTESDEAAVKRYLREYLAELGFN
                     REDFRENGKMYELGKYQGTIRKGYGKRKSLMKQYIKACKNEYKKDYREKKLDKSELDR
                     ILNNIDKFYYAFYSLSILLDMQRKVKILDSIAYYLRVLIREGLWVHGLYGYAARYLYD
                     FNIFDTTPYARALLERFHEFESGPKGALTRYIVSLDDKSQEYIESLKDMVFNLSDKKS
                     YDDVYLENIINYFEQLQNARGYVTRCYMLLAVFIYLIRRNKLHKALRFYDESQKYELP
                     FGYLPGAFSVLRIALEIKVNREKIKHGSLFELLDYVKAYQDAFMDLRVVTDPAYNEDE
                     IQYDANNFTLMRVIKMYNSMLANISTKSDIQPPYITGLLDNVERALDKINILIDKERV
                     YDGETLAELITENKILSSRESKENLIGLFTGRHKYTLLQCIEKLGVLVDYVISPADDI
                     KNVMMLYGNNAENKNRRRLIYNALTIICGDDTKNNQSDPR"
BASE COUNT         1358 a         1031 c          828 g         1242 t
ORIGIN      
        1 gcagtcctgg cacaaatctg gctggtacct gtaaacagac ttcgtacatg ttgctctgca
       61 ataaatcccc gtaccacagg cctcacagaa tttcttccca tgaaaaaact gctcacacat
      121 cacacaatgc cactccagca cgaccggtaa tggcgataga aacatcgcca tatcctcaat
      181 gtaagggtgg gacttttccg gattcagcac cacgcaggtc gccttctgtt gcgcgctcag
      241 ggcatgtgaa tcatgctcaa accacgcccc ctgagcatct gtctgcaaaa tcaaccgacc
      301 acgacaggaa aggcagaaac actgccggat atttctgctg agcgcagagt cggcactgat
      361 aatgtgttca cccggcgtga tccccagccc cgtttttata ccgttcattc agccactccc
      421 tcctcactga agtgccctgt atggcagtgt gtgcagtacc gctccccata ataatcgtgg
      481 tgacattgtc tgcagcgcca gctggcttta cgcaccacgg gtaaggcatc cgggacgaat
      541 tgctgcagac gtttaatcaa ccgtatttct ctgcgttccg gcctgacata aggacattgc
      601 tgcgcgtgct ccgtcagccc ttcgtctgcg tgttcaaacc agggacgctc agtgtcgtat
      661 tgcgggtggt atctgagcgc actgccgcaa aggtggcaag tgtagcgatc gtaaggtgca
      721 gtctgtgcgg tacgggcacc cgtcagacgc ccgttgccat caaatgcaat aaaggatttt
      781 gcgtacatcg tcatattcct cagccgccag acgacacgca ggcaacagct gtcccggtac
      841 gggcagtgtg gtcagggtgt gaatggcggt tcagttaaaa tctggactgg agtttgggta
      901 aaacaggctg gttgctgtta ccgcactgtg gttgtcacac aatggcccac ctgcgtaaaa
      961 aactgtcagc aggcagggat ggacatcggg ggatgtgctg tatcggttgc cggaaggaat
     1021 ggtaaagaat gtgccatggt taaatctccg tatggttcat acggaatagc catgtcgtaa
     1081 aaaatgcgca gagtccctga agtggcctcc ggaaccgact gtaacaatta gcgccagccc
     1141 gtagttgtcg ttcaggggct tacaccagag aacatatctc cgacagggta ctgtccagtc
     1201 gcggtcggcc ccggttactt ttttcgggaa gcctgctacg ccccagaata ataccgtgtt
     1261 ctttctgatc ctgtttagcc aggtagctga gggcgtaacg taagtcgtct accgcagatt
     1321 tatcactata gtgaatcacg tgatcgatac gcaccgggta tttgtcttta gcccggcaca
     1381 gatgaaaata accctcccct tccgtgatcc gcctccagat atctcccagt tgcctggata
     1441 tctgataaga gttcttgtga cgttgtccat ccagatagcc gataaaatga atatgaagcc
     1501 ctttgtctgg tgtatattcc attacccagt aatatcctgc taacatcgtc tgggtttcgc
     1561 taagtaaacg gtatatttcc atacacatac tgtgcttaca ggaatgcccg aaactgggcg
     1621 tgtctttcct gtaggcaaaa tcaattctga aaggtaacag tttagaaaaa cgttgaaaca
     1681 taccgtccat gtgttcattc acatctttca gaatcgcgaa attcatttcg taattgggat
     1741 tggcattata cattttatag tccttacttt ataaaactca caggaagagg aaattatgta
     1801 aatgctgagg gtacagaaac gtcgtactac ggcattaata aaagagataa ccagcagcac
     1861 agacaacctg ataaaactgt tttgtattac ctcattacag caatgaggta atatttcaga
     1921 atacagtgag actctcttaa actgaggacc agtacaaatt acaatggtaa aaacaccacc
     1981 tcatagaatt gagtttaaga aaacaccatt cactcaacct gacattcaca aaagtcagta
     2041 ccaggtagcc tcaggtaaca tcgctattaa tattatcctg ccattaagta aaaacagaaa
     2101 ctctgtgagg aagcctcacc aatatttgac cagtagcata aagaataacg atatgagaga
     2161 tacgcccaca ccagagatga ttagcccata caaaaagata atatttatta ataccaccga
     2221 acctcaatag agtaataaca cactaatagc gttttagtac acacttaaca ctaactatgc
     2281 accagacaca tactcaggat taacattctc aacatttatg gtactctcaa agagtataat
     2341 tcaccacaca ggtaaactct cacaccagta gtatggagat aatgctgtac acatacttac
     2401 agagtaaagt gtacagctag catacagtgg cgagcagaca ttacaacata aagactcaag
     2461 atagcataat aaagcataat aagaacacaa caaaactccc actaataata ctaattaacc
     2521 ttaattcttt tttgaacagg aaatcaacat agctccagac ctcttcaggt catacacttt
     2581 tgtattggat gttcaatgtg tacttctgca cagacaaaaa aataaaaccc acaaaataaa
     2641 atatcaatcc caataaaaaa ttttaaaata cagcgtgaaa caccacgtta tggatatgtt
     2701 ttcatatcac cagaagcata tttcacacat acaatacagg aacaattcat catataatca
     2761 gggttactgt tcactcctca cggttatccg cgagacgata aaacatgaca ctaatcacaa
     2821 aaacgagaga tagtatttgc aaaatgcaaa caagataaaa atcaatgtgt tgcgattttt
     2881 tattttttca gacatgcatg ccccctctgc tattgttgct agccgactag caacaatagc
     2941 agggatatac aaacatcgga tgccaaaaac acgttccgat aatatcatct agggtctgac
     3001 tggttgtttt tagtatcatc accacaaatg atggttaatg cgttgtagat cagtctgcgt
     3061 ctgttcttgt tttcagcatt atttccatac aacatcatga cgtttttaat atcatcagcc
     3121 ggtgatatta cataatcaac caatacgcca agcttctcaa tacattgcag taaagtatat
     3181 ttgtgtctgc ccgtaaataa tcctatcagg ttttctttac tttcacgact ggataatatt
     3241 ttgttctctg taattaattc agccaacgtt tcaccatcgt aaacacgttc tttatctatc
     3301 agaatattta tcttatctaa cgccctctct acattatcga gaagacctgt aatatacgga
     3361 ggctgaatat ctgactttgt actaatattt gcaagcatcg aattatacat tttaatgaca
     3421 cgcatcagag taaaattatt cgcatcatat tgtatttcat cttcattata cgcaggatcc
     3481 gtcacgaccc ttaagtccat aaatgcatcc tgataagctt ttacataatc aagtaattcg
     3541 aacagtgaac catgctttat tttctccctg tttactttga tctccagcgc tatcctgaga
     3601 actgaaaaag cgccgggaag atacccaaaa ggtaactcgt atttttggga ttcatcataa
     3661 aaccgaagag ctttgtgaag tttgttccgc cgaatcagat aaataaaaac ggccagtaac
     3721 atgtagcacc tggtcacata accacgggca ttctgcaact gttcaaaata attaataatg
     3781 ttttccagat atacatcatc ataacttttc ttatcagaaa gattaaacac catatctttt
     3841 aaagattcga tatattcttg tgatttatca tcaagtgaca caatataacg agttaatgct
     3901 cctttggggc cactctcaaa ctcatgaaac cgttcaagta atgctcttgc ataaggcgtt
     3961 gtgtcaaaaa tattaaaatc ataaagatag cgtgcagcgt aaccatacaa cccatgtacc
     4021 caaagccctt ctcttatcag cactctgaga taatatgcaa tactgtcaag tatctttacc
     4081 ttccgttgca tatcaagcaa aatacttaat gaataaaatg cataatagaa cttgtcaatg
     4141 ttgttgagta ttctgtccag ctcactctta tcaagcttct tctctctgta atccttcttg
     4201 tattcattct tacaggcctt tatatattgt ttcataaggc tcttcctttt accgtaccct
     4261 tttcgaatag ttccctggta ttttcccagc tcatacattt ttccgttttc ccgaaaatct
     4321 tctctgttaa atcccagttc agcaagatac tcccggagat aacgtttaac ggcagcttca
     4381 tcgctttctg taccagagta atccaggcag ggacaatatg atttttgcaa aaaattatag
     4441 atatgataat aaaaccctg
//
LOCUS       DAZGQL010000199         4409 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.201, whole genome shotgun sequence.
ACCESSION   DAZGQL010000199 DAZGQL010000000
VERSION     DAZGQL010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4409
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.201"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(1..1123)
                     /locus_tag="ACHKIR_005019"
                     /pseudo
     CDS             complement(1..1123)
                     /locus_tag="ACHKIR_005019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700772.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
     gene            complement(1389..3257)
                     /locus_tag="ACHKIR_005020"
     CDS             complement(1389..3257)
                     /locus_tag="ACHKIR_005020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992623.1"
                     /translation="MGMFNYKIYNAAHVTYLVSSSYKLAIYSNTGKVLGLGSFLASKL
                     FAKHPDKIDYSPDALNEGWRDITVEELRLSSEYVDSRGFYRFPSPVTGNPASGFGPQL
                     KVVGLFDAQQNLQKIAISFCGTNDICDIVDYTQLNNGKALSEAENLLQSIRDFAISNG
                     LSGEDVIVTGYSLGGGFTNIMARLSESLADGFYANSDYIAWASPLIFDGDGKVLNIGF
                     ENDPVFRVLGDESGFWEAVKAMGPGLTTPDREYDSSPDNIVLYTDLYGSFLWNIIPFS
                     VLALPLGSWSAHFAGSKTDAFERILGSQFYDYMSPDSTVIVDEQSGWTSWYSWVHDKG
                     DDSRERPVFIIGNDGNNLLAGGQGGDYIEGAGGNDKIEPGKGANRVDGGSGYDTLLLG
                     DVMDDWNIYRVSDGTLFFHSERGAGLTEATGIEGISFSGDWRSKLSPYQIEEEQITDH
                     RYLLKWRNKNVDYGKHTEGTNGDDSLFGNIIFGLDGNDILHSAGGEGVSLLHGGRGND
                     YLHGASGNDELYGAEGNDWLYGGGGNDYLYGGTGHDVFVFDAACEGNSFIMDFNKHKG
                     EKDFLFLSKDIVTSTDEFISCLKQTGNDVEAGIAGGQIYFVNTALEDINHNSVYIV"
     gene            complement(3336..>4409)
                     /locus_tag="ACHKIR_005021"
     CDS             complement(3336..>4409)
                     /locus_tag="ACHKIR_005021"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992624.1"
                     /translation="KADAEEAAEARVFYEKAFSNVYQTDDLYARTDTTSLFAPAAIKL
                     AKSTAQWATILEKNAGAQMSQDQNAGGETRYIYNGISGSDVITVGKSLGGTGLNMTAT
                     RNDMKVMTGDGDDIIITGQDYGRLASAGQWDYKYLTEMGNGNDTLIVGASNSNLNVIM
                     FNDGSIAAVKKDGAQLGSVIPFDSAYDTADGGNISGTTIDMGSGNDTVLALGHENGGT
                     AIINSTIKLGAGNDTIQINGDVKGGKSPSVITGDAGMDTLIISNGSVYSEHFSGFENI
                     ELGSKGEVKIVAADLVGKDSNSIQGGMLKIIGNSDSKVDLDGSDWIKGEIKNEGDITY
                     NVYTHASAPNISVLIEDKITQVI"
BASE COUNT         1241 a         1191 c          771 g         1206 t
ORIGIN      
        1 tcgttaaagc gatatcccat gcccaccgca acagcctgct tgtcactgta cccgcccaca
       61 gccgctgtgg cgttaaattt acccacactg tatggctgga aaagaccggc cagcgctgct
      121 gactgggctg ctgcccgctt catttcctca tgattctcat taatctgacg ctgctgactg
      181 ttcatacgac gctcatgcgc ctgcagggta cgtgcctgag aagcaatggc gcgactgttg
      241 cgttcaccca cctgacggtt acggcttacc gcagcacggt tttctgtggc catttcctgt
      301 gtacgtacca gcccttcctg tacagtaatt cgttcaccgg ttccctcacc gcgcgtcaca
      361 taaccatgac tgtccacagc aacggtactt ccggtattta tctgctgttg ctggctgttc
      421 aggcgctctt catgcgtctc cagcacgtct ctctgggaaa gcagcccctg gtgttacggt
      481 ttatggccac cgtgttccgg ataatattct ccctgttctc tgcagcaacc gtatcggtat
      541 ggtcacgcgc cttaagtgct tccgctgcaa tccgtccact gacgtcactg tacacctcat
      601 ccgccttccg gttcagttgc ccgacagtgg ctgcatccgt atcccgtgta ccatcactga
      661 caccaccgag aacccgggtt ttatccggta cacgcggact ggccgggttt gtgacacctg
      721 agtcatatcc gatgtagact tcattttcac gggttgtacc ggactgctga ccaagagcca
      781 ctgaatacct gtgcattgcc tgtgcacccg tacccagtgc tactgaattt aaagcatctg
      841 cagatgccca gctacctact gccacaccat tagacgcctc cggatttaca taagcagaca
      901 tccccacgga tgtggaaaac ggcgcatgcg catacgatgc agagccaaga gctgtccctg
      961 ccttcccggc attcgcccat gcccccactg ccgtggagtc agtctctgat gcagaggctg
     1021 tgtccggaga cgcccaggaa ctgccatcct gcgcaaacgc cggagcaccg gcaatcagaa
     1081 aggaaataag cgataaagta ataccgtgtc gatttatttt cataaacaac ctcattgata
     1141 tacacaatat actggcacag agttaaatta atatataatc aggagagaag tgaaagagta
     1201 cccggtgcga aaatgaatta atctgagcag atttaacggt aaacgaagcg caataaatta
     1261 aaattaaaaa acaaaatcac ttgcagtcta ttatctttac gttcctcacc gcaaccgcac
     1321 acaaaaacaa aacacacaat gcattgattt taaacaaaaa aacaaaacag aaactcaaca
     1381 cataaaaatc agacaatata aacagaattg tgatttatat cctcaagcgc tgtattaacg
     1441 aaatatatct gtcctcctgc aatacctgcc tcaacatcat ttccggtttg tttcagacat
     1501 gaaataaact catctgtcga tgtaacaatg tcctttgata aaaacagaaa atctttttca
     1561 cccttatgtt tattaaaatc catgataaat gaattccctt cacaagcagc atcaaagacg
     1621 aagacatcat gaccagtacc accatacaga taatcattac ctcccccacc atataaccag
     1681 tcgttacctt ctgctccgta caactcatca ttaccagaag ccccatgaag ataatcattc
     1741 ccgcgacctc cgtgtaaaag acttactcct tctcccccgg cactatgtaa tatatcattt
     1801 ccgtcaagac caaaaataat atttccgaac agactatcat ctccgtttgt accttctgta
     1861 tgttttccgt aatcaacatt tttattacgc cacttcagaa gatacctgtg gtctgtaatc
     1921 tgctcttctt ctatctgata agggcttagt ttactcctcc agtcaccaga aaaagagata
     1981 ccttcaatac ctgttgcctc agtaagaccg gctccccgtt ctgagtggaa aaacagtgtc
     2041 ccgtcactga ccctgtatat attccagtca tccattacat cgccaagaag aagcgtgtca
     2101 tacccactgc caccatcaac cctgtttgct cccttacctg gttcaatctt atcattccct
     2161 cctgccccct ctatatagtc accaccctgc ccacctgcca gcagattatt gccatcatta
     2221 ccgatgatga atacaggtct ttcacgactg tcatccccct tgtcatgaac ccatgaatac
     2281 caggatgtcc agccactctg ctcatccaca atgactgtac tgtccggtga catgtagtcg
     2341 taaaactgag accctaaaat tctttcaaag gcgtcagttt ttgaacccgc aaaatgcgca
     2401 ctccagcttc ccagcgggag agcaagcaca gaaaagggga taatattcca gaggaatgaa
     2461 ccataaaggt ctgtgtataa aacaatgtta tcaggagaag agtcatattc acgatccggg
     2521 gttgtcaagc ccggccccat tgctttaact gcctcccaga acccggattc atctcccagc
     2581 acacgaaata ccggatcgtt ttcaaaaccg atatttaata ccttcccatc cccgtcaaat
     2641 atcagcgggg aagcccaggc tatatagtca gaattagcat aaaaaccatc agccagagac
     2701 tcactgagtc tggccataat gttagtaaac ccgcccccca ggctatagcc tgtgacaata
     2761 acatcttcac cacttaaacc attactgatt gcaaagtctc tgatactttg aagcaggttt
     2821 tctgcttcag acagcgcttt tccattattg agctgtgtat aatcaacaat atcgcagata
     2881 tcatttgttc cacagaatga tattgcaatt ttctgtagat tctgttgagc atcaaacaac
     2941 ccaacaacct tcaactgagg accaaatcct gacgcagggt ttcctgtaac cggactggga
     3001 aagcgataaa aacctcttga atcgacatat tccgaagata acctgagttc ttccactgta
     3061 atatcacgcc agccttcatt aagagcatca ggagagtaat caattttgtc tggatgcttc
     3121 gcaaatagtt tagatgccag aaaactgccc aatccaagaa cctttcctgt gttgctatat
     3181 atagcaagct tataagaaga agaaacaagg tatgttacat gcgcagcatt ataaatttta
     3241 taattaaaca tccccataca gactccttta aattaccaaa tctatatatt aagaggccac
     3301 gcatacagcg cggccttgtc actatttagc tcaaatcaaa taacctgagt tatcttatct
     3361 tcgattaaca cgctgatatt aggtgcagat gcatgagtat aaacattgta agttatatcg
     3421 ccctcgttct tgatttcacc tttaatccag tcacttccat ccaagtcaac cttagagtct
     3481 gaattaccta tgattttcaa cattcctccc tgaatggagt tactatcctt tcctacaaga
     3541 tctgctgcta ctatttttac ttcaccctta ctacctaact caatattctc aaagccagaa
     3601 aaatgttcag agtatacact accattacta ataatcaatg tatccatacc agcatcacca
     3661 gtgatgacag aaggagattt acctccctta acatccccat tgatctggat agtgtcatta
     3721 cctgctccaa gttttattgt tgagttaata atagcagttc caccattctc atgccccaag
     3781 gctaaaacag tatcattacc acttcccata tcaattgtag ttcctgatat attacctcca
     3841 tcagctgtgt cataagcaga atcaaaagga attacactcc ctaattgggc accatctttt
     3901 ttaactgcag caatactacc atcattgaac ataattacat tcagattaga gtttgatgcc
     3961 ccgacaataa gagtatcgtt tccattcccc atctctgtga gatatttata atcccactga
     4021 ccagcacttg ccagacgacc ataatcctga ccagtgataa ttatatcatc gccatcacca
     4081 gtcattacct tcatgtcatt acgcgtcgct gtcatattta agcctgtgcc acccaagctt
     4141 ttgcctactg taataacatc acttccggaa ataccgttat atatgtatcg tgtttctccc
     4201 cctgcattct gatcttgaga catctgagct ccagcattct tctcaaggat tgttgcccat
     4261 tgggccgtgg atttagccaa ttttattgcc gcaggtgcga ataatgatgt tgtgtcagtt
     4321 ctagcgtaca gatcatcggt ctgatacaca tttgagaatg ctttctcata aaagactctg
     4381 gcctctgccg cttcctctgc atccgcctt
//
LOCUS       DAZGQL010000200         4299 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.202, whole genome shotgun sequence.
ACCESSION   DAZGQL010000200 DAZGQL010000000
VERSION     DAZGQL010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4299
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.202"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            211..489
                     /locus_tag="ACHKIR_005022"
     CDS             211..489
                     /locus_tag="ACHKIR_005022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000106368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992625.1"
                     /translation="MSTNTPTEINKLFTDPAHIELTQKTLTEITNVLDERISEIDKDK
                     HFATYMALQMQSMAMVTAKQTISELYMKNLRLERELAELRAQSGQFSA"
     gene            501..743
                     /locus_tag="ACHKIR_005023"
     CDS             501..743
                     /locus_tag="ACHKIR_005023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001084373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992626.1"
                     /translation="MNSIFTATMLTRFTDAVGHEFMVESHLITTTTPCPSDADYLYIH
                     LADGTQITAIASTVREVTAIRGAWKSETQAHGELRP"
     gene            740..952
                     /locus_tag="ACHKIR_005024"
     CDS             740..952
                     /locus_tag="ACHKIR_005024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992627.1"
                     /translation="MISKYTTNARKPGKDARHHIAVLRNLLLEEIDDFTATLSGPGAP
                     VTPGEIHRDLKRRIENAIDYTLNPTE"
     gene            979..1533
                     /locus_tag="ACHKIR_005025"
     CDS             979..1533
                     /locus_tag="ACHKIR_005025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000478924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SANT/Myb domain-containing protein"
                     /protein_id="HFO9992628.1"
                     /translation="MRLTPMGIPGKDPAHVKAWTQQEDRLLISLYQEHNTHQMAERIQ
                     RTRKAVMYRIRFLRNRGLIGRKRKAPLSTEAIAFLIKNRHVKTAQEMALEVGCSECTV
                     RYTLRKRGYNLKKCGENHHCARYSDHLVELVTELRDIHNMTFCMIAEHINSTIQMHIN
                     DDAAFHLYHRRTATDAVLCELLPG"
     gene            1543..1923
                     /locus_tag="ACHKIR_005026"
                     /pseudo
     CDS             1543..1923
                     /locus_tag="ACHKIR_005026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372767.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1854..2081
                     /locus_tag="ACHKIR_005027"
     CDS             1854..2081
                     /locus_tag="ACHKIR_005027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="derepression protein"
                     /protein_id="HFO9992629.1"
                     /translation="MANRKQQRARAERLHIRTEINRRLHRAASVTFIMQSNALHRLND
                     GIAAGYYAAVFSYLAEDLAELQDLINQQHHH"
     gene            2150..2560
                     /locus_tag="ACHKIR_005028"
     CDS             2150..2560
                     /locus_tag="ACHKIR_005028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992630.1"
                     /translation="MKSVKGLSDIQDFFNWMWAVDPRLASRLNDWHDYYRRACKQAAR
                     HVTTYGDFSFTVDDRYRVDVKGDGFGLFHINGTDSTAISWSSSEMTLVLEMTNHSVAV
                     KGIYTAEDFIGEYQRLLVLYRAAFQAATAGECDA"
     gene            2557..>4299
                     /locus_tag="ACHKIR_005029"
     CDS             2557..>4299
                     /locus_tag="ACHKIR_005029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFO9992631.1"
                     /translation="MSDHNAQLAYPWNAPLPVIDPETFGRAQPTALRQAIQSYINEDI
                     RTDARLDEETVAFLTTTDEGKRINSHLYHDEERRIRLEKLARHKHENPPTLVSEAMAE
                     LRALPPFLAGPLIRDLARLSRKQESARREGLKNNSAYAADNFVRHGLRARLKRIARVN
                     ERFATPAFKATAARERLDELLMLPQLSRDEIQRLATLTATTFSTALERACDEVIEKTG
                     RGDDDLLTWLLTYQPLAHMALKLGITPPCWPSLEIRPDRRTDPDPERVPGAVMRLSCA
                     TWWNSQLRHLRDIWREELLRAAGLVSRKTSVYISHEALLDWREKRQRTRDFLKAWDIE
                     NEEGERFSLEDIYWAGLSNPRNRRNEMMTCARGMEQVAEARGDVAFFVTVTAPSRFHS
                     VNDDGSLNPKYCGATVRDASDYLVYRFFAAVRRAIDKAGLGWYGVRTVEPHHDGTPHW
                     HMLVFTSQENEARITEIMRNAAIREDRAELGDDISPRFKCKKIDPAKGTPTSYIATYI
                     GKNLDASTFMGNDPKTGKPYVDKESGKTMAETVENAIGWAGLHRIHQFQFFGIPPRQV
                     WRELRRLAGQMARNP"
BASE COUNT         1116 a         1244 c         1116 g          823 t
ORIGIN      
        1 gcagcatgag cgtatttctt tcatgggtga ttctgattat ttccgtgctt atggcgattg
       61 gcattatgcg aattatcgaa gcgctgaaaa atatcgaatc tttatttact ggcgaagaac
      121 gccgtaaata aataaccaac cggacagaaa acattaaaac catccgcatc tgcggagggc
      181 ttcactcaat ccagaaacgg agaaacagct atgagcacca atacacctac agaaataaac
      241 aaactattta ccgacccggc gcatattgaa ttgacgcaaa aaacgctgac tgaaataacc
      301 aatgtactgg atgaaagaat atcagaaata gataaagaca agcattttgc cacctacatg
      361 gccctgcaaa tgcaaagcat ggcaatggtg acagcaaaac agaccatcag tgaactctat
      421 atgaaaaacc tgcgcctcga gcgcgaactg gcagaactcc gggcacagtc cggccagttt
      481 tcagcgtgag gggcaggtcg atgaacagca tatttaccgc caccatgctg acgcgtttca
      541 ccgatgccgt gggccacgag ttcatggtgg aaagtcattt gatcacaacg accacgccgt
      601 gcccgtcaga cgctgattat ctctacatcc atctggctga cggcacacaa atcaccgcga
      661 ttgccagtac cgttcgcgaa gtaacggcca tcagaggggc atggaaatca gaaacacaag
      721 cccacggaga gctgcggcca tgatcagcaa gtacaccacc aacgcacgta aacccggtaa
      781 agacgcacgc caccacatcg ccgtactccg caatctgtta ctggaggaaa tcgacgactt
      841 cacggcaacg ctgagcggtc cgggcgcacc agtaacacca ggtgagatcc accgggacct
      901 taaacgccgt attgaaaacg ccattgatta caccctgaac ccgaccgaat aaccggaaca
      961 gtaacggagg ttatcgcgat gagacttaca cctatgggaa taccgggcaa agacccggca
     1021 catgtgaagg cgtggacaca gcaggaagat cggttactca tctcgcttta tcaggaacac
     1081 aacacacatc agatggctga acgaattcag cgaacccgta aagcagttat gtaccggatt
     1141 cgttttttac gtaatcgtgg gctgattggc cgcaagagaa aagcaccact gagtacagaa
     1201 gccatagcct ttcttattaa aaaccgtcat gtaaaaaccg cacaggaaat ggctctggaa
     1261 gtgggatgca gtgagtgcac agtgaggtac acactgcgta aacggggtta caacctgaaa
     1321 aaatgcggtg agaaccacca ttgcgccaga tacagcgatc atctggttga gctggtcacg
     1381 gagctacgcg acatacacaa catgacgttc tgcatgatcg ctgaacacat caacagcacg
     1441 atacagatgc acattaacga cgatgcggcg tttcatctct accaccgccg caccgcaact
     1501 gacgcagtgc tctgtgaact gttaccgggc tgagtgatca tcatgcacac acaaaaaaac
     1561 cgcttgccag cgttcgcctg gtcgggttac agttttcccg cacctcataa aacgggtgcc
     1621 gggattacca ccccgccgac tacaagagcg cacaaccgcg ccagcggttt ttttgtgcgt
     1681 actgtatcgc cacgtctttt tcgcgtcaga attatggcgg ggcgtacggg gccgacttcg
     1741 gtcgggccgg gatctcttgt agccggttgt ggtaaccctg tacgtctcgc caccctgaga
     1801 ttaccgcctc gggttggtga gttttcagaa cttactacaa gaggccaaac accatggcaa
     1861 accgcaaaca acagcgcgcc cgcgctgagc gtcttcacat ccgcactgaa attaaccgca
     1921 gactccatcg ggctgcatct gtcaccttca tcatgcagtc aaacgcatta catcgactta
     1981 acgatggtat cgctgccggg tattacgcgg cagtcttcag ctatctggcc gaagacctgg
     2041 cagaactgca ggaccttatc aaccagcaac accaccacta aacaacatcc tgaagccatc
     2101 ccgcacccgt gcgcgggagg cttccgcacg tctgttaccg gaggttctca tgaagagcgt
     2161 taagggactt tcagatattc aggatttttt taactggatg tgggcagtgg atccccggct
     2221 ggcttcccgc ctgaacgact ggcacgacta ttaccgccgc gcctgtaaac aggccgccag
     2281 acacgtcacc acatacgggg atttctcctt caccgttgac gaccgttacc gcgtggacgt
     2341 gaaaggtgat ggttttggtt tatttcacat caacggaacc gacagcaccg ccatcagttg
     2401 gtcgtcgtca gagatgacgc ttgttctgga aatgacaaac cacagcgtgg ccgtgaaagg
     2461 tatatacacc gcagaagact ttatcggtga ataccagcgc cttctcgtgc tgtaccgcgc
     2521 ggcgtttcag gcggcgactg ccggggagtg cgacgcatga gcgaccataa tgcacaactg
     2581 gcgtacccgt ggaacgcccc gctaccggtt atcgatcctg aaacgttcgg cagagcgcag
     2641 ccaaccgcat tacgccaggc cattcagagc tacatcaacg aggatattcg cactgacgcc
     2701 agactggatg aagaaaccgt cgccttcctg acaaccacag acgaagggaa gcgaattaat
     2761 tcgcatcttt accacgacga agagcgtcgg atcaggctgg aaaaactggc gcggcacaaa
     2821 cacgagaatc cgccaacact ggtatctgaa gccatggcag agctgcgcgc cctgccgccg
     2881 tttcttgccg gtccgctcat ccgtgacctg gcgcgcctga gccgcaaaca ggagagcgca
     2941 cgccgggaag ggcttaagaa taacagcgca tatgcggcgg ataacttcgt tcgtcacggg
     3001 ctgcgtgccc gcctgaaacg tatcgcccgt gtgaatgaac gctttgccac cccggccttt
     3061 aaggccaccg ccgcgcgcga acggctggat gaactgctta tgctgccaca gctgagccgt
     3121 gacgaaatcc agcgccttgc caccctgacc gccaccacgt tttccaccgc actggagcgc
     3181 gcatgtgatg aagtcatcga aaagaccggc aggggtgacg atgacctgct cacctggtta
     3241 ctgacctacc agccactggc acacatggcg ttaaaactgg gtatcacgcc accctgctgg
     3301 ccgtcgctgg aaatcaggcc cgaccgccgc accgaccccg acccggaacg ggtgccgggt
     3361 gcggtcatgc gcctttcctg tgcgacatgg tggaacagtc agttaagaca cctccgggac
     3421 atctggcgtg aagaactgtt acgcgccgca ggtctggtgt cacgtaagac atcggtatat
     3481 atcagccatg aagcgttact ggactggcgc gaaaaacgcc agcgcacccg cgattttctg
     3541 aaggcctggg acatcgagaa cgaagaggga gaacggttca gccttgagga tatctactgg
     3601 gcaggtctct ctaatccgcg taaccgccgt aacgagatga tgacctgcgc ccggggaatg
     3661 gagcaggtcg ccgaagcacg cggcgacgtg gcgtttttcg tcaccgtcac agccccttcc
     3721 cgttttcaca gcgtgaacga tgacggcagc ctgaacccga aatactgcgg agcaacggta
     3781 cgcgatgcca gtgattatct ggtttaccgc tttttcgccg ccgtccgcag ggccatcgac
     3841 aaggccgggc tgggctggta cggcgtgcgc accgtggagc cccaccacga cggtacaccg
     3901 cactggcaca tgctggtatt caccagccag gaaaacgaag cccgtatcac tgaaatcatg
     3961 cgcaacgccg ccatcaggga ggaccgcgca gagctgggtg atgatatctc cccgcgtttt
     4021 aaatgcaaaa aaatcgaccc ggcaaaaggc acaccgacaa gctatatcgc gacctatatc
     4081 ggtaagaacc tggacgccag caccttcatg gggaatgacc cgaaaaccgg caaaccttac
     4141 gtcgataagg aatcaggcaa aaccatggcc gaaaccgtgg agaacgccat cggctgggca
     4201 ggccttcacc gcatccacca gttccagttc ttcggtatcc ctccccgcca ggtatggcgc
     4261 gaactccgcc gtcttgccgg gcaaatggcc cgtaacccg
//
LOCUS       DAZGQL010000201         4254 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.203, whole genome shotgun sequence.
ACCESSION   DAZGQL010000201 DAZGQL010000000
VERSION     DAZGQL010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4254
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.203"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..801
                     /gene="tssC"
                     /locus_tag="ACHKIR_005030"
     CDS             <1..801
                     /gene="tssC"
                     /locus_tag="ACHKIR_005030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017892320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HFO9992632.1"
                     /translation="AFRETDDARYIGLVMPRVLGRLPYGPDTVPVRSFNYVEEVKGPD
                     HHKYLWTNASFAFAANMVRSFVTNGWCVQIRGPQAGGAVQDLPIHLYDLGTGNQVKIP
                     SEVMIPETREFEFANLGFIPLSYYKNRDYACFFSANSTQKPALYDTPDATANSRINAR
                     LPYIFLLSRIAHYLKIIQRENIGTTKDRRLLELELNNWIRGLVTEMTDPGDELQALHP
                     LRDGKVVVEDIEDNPGFFRVKLYAVPHFQVEGMDVSLSLVSQMPKAKA"
     gene            817..2154
                     /gene="tssK"
                     /locus_tag="ACHKIR_005031"
     CDS             817..2154
                     /gene="tssK"
                     /locus_tag="ACHKIR_005031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HFO9992633.1"
                     /translation="MKIYRPLWEDGAFLMPQQFQQQAAWDVHLADSVARMGLAHPWGV
                     VAAEFDDSLLPLSRLNATRLIVRFPDGTLIDTERADNLPPVCDLSTVSERSLVDIVLA
                     LPLLNANGGNLDDGSESERPRRWKSERVNVQELAGHEQSEVAVLRHNLTLRMAHQENA
                     AWLTCPVTRLVRDAQGQWCKDPRFIPPLLTLSASPSLMTELAELLHHLQARRQRLMSM
                     RRENNARLADFAVADVSLFWLLNALNSAEPVLKELLDMPYRHPELLYRELARLAGSLL
                     TFSLEHNVDAVPAYRHETPENVFPPLLSLLNQLLEASLPSRVVFIELKQNGVMWEGAL
                     HDARLREGADFWLSVRSSIPGHELQTKFPQLCKAGSPDDVSEVVNVALSGVIIRPVTH
                     VPAAIPLRLENQYFALDLSTDAARAMLDAGRCTFYTPASLGDVKLELFAVLRT"
     gene            2151..2804
                     /gene="tssL"
                     /locus_tag="ACHKIR_005032"
     CDS             2151..2804
                     /gene="tssL"
                     /locus_tag="ACHKIR_005032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssL, short
                     form"
                     /protein_id="HFO9992634.1"
                     /translation="MNKPVISRAEQIFYPGWLMVSQLRCGQPVEDGKALYRRACQLVT
                     QAREELAEAEFSQESSEIMLYAFCALLDESVLNREKTDDGWHTWQQDPLQAHFFGTLN
                     AGEELWERIREQLKLPAPDVAVLTCLCRTLQLGFTGQYRSQDDERREDVIRALTARVP
                     AFTFAQDAPVVVRAPGYRAGRTMYWLSWVAGIVTLAGLWCVLSSVLADQVARLTGQG"
     gene            2807..>4254
                     /locus_tag="ACHKIR_005033"
     CDS             2807..>4254
                     /locus_tag="ACHKIR_005033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000558483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFO9992635.1"
                     /translation="MHVRSRLWLTILSLFLTLWLISGFLPLSAVNQIILTLVSVVAAG
                     LVLWYQWYCLSSRRDAPEKAGDDGLPPEYFQGHVLLVAGNSDAWFEPGQAYRETASGW
                     YLRVDTPEQMRILAERLSYSRPALCGQVVVMLGLLPEQHTSDEAFLSWLRNWQRAVMQ
                     CRQWLNTMPPVLVTLMVSGPSPQNTSLNKHSAHWFTLTPDLSGMHIRLTEAGAVPAEE
                     WLRECDAASRLRCVSELLWLNAMLSWYNRVTDALTDICRQEHFYFRPVAVGFCLTSVA
                     ARPGNLWQQQLTSLTVLPPPAGVSSQALPLPDILLSGIPRHRGLSHWHRLCLQTGAIV
                     GVFLLLAMLASFMNNQRLVRSVGEHLTAWHRMRGYSSESKKTALQRLQADSQLLNDWQ
                     QRGVPQRYGLGLYQGMWLIPPVERATGEWIAPLPPRPVIQKVTQTPKMVRLNSLALFD
                     AGKWTLKPGATKWLVNALVDIKAKAGWLIVVSG"
BASE COUNT          844 a         1074 c         1286 g         1050 t
ORIGIN      
        1 gctttccgtg aaacggatga tgcccgctat attggtctgg tgatgccacg tgttctggga
       61 cgcctgccat atggtcctga taccgtacct gtccgcagct ttaactatgt tgaagaggtg
      121 aaaggtccgg atcatcataa gtatctctgg acaaatgcgt cttttgcttt tgcggccaat
      181 atggtgcgca gttttgtcac caacggctgg tgtgttcaga tccgcggtcc tcaggccggc
      241 ggtgcggtgc aggatttgcc gatccatctg tatgatctgg gaaccggtaa tcaggtcaaa
      301 attccgtcag aggtgatgat tccggaaact cgggagtttg aattcgctaa tctgggcttt
      361 attccgctct cttactacaa gaaccgggat tatgcctgct tcttctccgc taactccacc
      421 cagaaaccgg cactgtacga tacgccggat gcaacggcga acagccgtat taatgcccgt
      481 cttccctata tcttcctgct gtcccgcatt gcgcattacc tgaagatcat tcagagggag
      541 aatatcggta ccaccaaaga tcgccgtctg cttgaactgg aactgaataa ctggatccgg
      601 ggtctggtga cggagatgac cgatccgggt gatgaactgc aggccttaca tccgctgcgt
      661 gacggaaaag tggtggtgga agacatcgaa gacaacccgg gatttttccg cgtgaaactc
      721 tatgctgtac cacatttcca ggtggaaggt atggacgtca gtctgtcact ggtttcgcag
      781 atgccgaagg caaaagcgta aggcaggcgt cgggaaatga agatttatcg tccactgtgg
      841 gaagacgggg cttttctgat gccccagcag tttcagcagc aggctgcctg ggatgttcat
      901 ctggcagaca gcgttgcccg aatggggctg gcgcatccct ggggcgtggt ggctgcagag
      961 tttgatgact ctctgttacc gctttcacgg ttgaatgcca cccgtctgat tgttcgtttc
     1021 ccggacggaa cgcttattga tacggaacgt gcggataatc tgcctccggt ctgtgattta
     1081 tcgacggttt ctgagcgttc cctggtggat attgtgctgg cgctaccgtt actgaatgct
     1141 aacggtggca accttgatga tggcagtgag agcgagcgtc cgcgtcgctg gaaatcagaa
     1201 agggtgaatg tgcaggagct ggccgggcat gagcaaagcg aagtggcggt attgcggcat
     1261 aacctgactc tgcgcatggc tcatcaggaa aatgctgcct ggctgacctg tcctgttacc
     1321 cgtcttgttc gagatgcaca gggacagtgg tgcaaggacc cgcgttttat tccccctctg
     1381 ctgacgctgt ctgccagtcc ttcgctgatg actgagcttg cggagttgct tcatcacctg
     1441 caggcgcgtc gccagcgact gatgtccatg cgtcgggaga acaatgcccg tcttgcggat
     1501 tttgcagtgg cggacgtgtc gttgttctgg ttgctgaatg ccctgaacag tgcggagcct
     1561 gttctgaaag agttgcttga catgccttac cgccacccgg aactgttgta cagggagctt
     1621 gcccggctgg caggctccct gctcacattc tcgcttgagc ataatgtgga tgcggttccg
     1681 gcataccgtc atgaaacacc tgaaaatgtg tttccaccct tgctttcttt gctgaaccag
     1741 ctgctggagg caagcctgcc ttcccgtgtg gttttcattg aactgaaaca aaatggtgtg
     1801 atgtgggagg gggcattaca tgatgcgcgt ttgcgcgaag gggcggattt ctggctttca
     1861 gtgcgctctt ccatacccgg gcatgaactg cagacgaagt ttccgcagct atgcaaagcc
     1921 ggaagccccg atgatgtgtc tgaggtggtg aatgtggcgc tgagcggggt cattatccgt
     1981 ccggttacgc atgtaccggc ggccattccg ctccggctgg aaaaccagta tttcgcgctg
     2041 gatttaagta cggatgcagc ccgggcgatg ctggacgcgg ggcgttgtac tttctacacc
     2101 ccggcatcgc tgggggatgt gaaactggaa ctttttgcgg tactgcggac atgaataaac
     2161 ctgttatctc ccgggctgaa cagatttttt atcccggctg gctgatggtc agccagctgc
     2221 gctgcggaca accggttgag gatggtaagg ccctgtatcg ccgtgcctgt cagctggtga
     2281 cgcaggcgcg tgaagagctg gctgaagcag aattcagtca ggaaagcagt gaaatcatgt
     2341 tgtatgcctt ctgcgccctg ctggacgaga gtgtgctgaa ccgtgaaaaa acagacgatg
     2401 gctggcacac ctggcagcag gacccgctgc aggctcattt ttttggtacg ctcaatgccg
     2461 gagaagagct ctgggagcgt attcgtgagc aactgaaatt acctgcgccg gatgtggcgg
     2521 tactgacatg cctctgccgt acgcttcagc tcggttttac cggtcagtac cggtcgcagg
     2581 atgatgagcg tcgcgaagat gtaattcgcg cactgacagc gcgtgttcct gcatttactt
     2641 tcgcacagga tgcgccggtg gtggtgcgtg caccgggtta ccgtgctggc agaacgatgt
     2701 actggttgtc atgggtggcg ggtattgtga cgctggctgg tctctggtgt gtgctttctt
     2761 ctgtgctcgc agaccaggtg gcacggctta ccgggcaggg ataaccatgc acgtacgttc
     2821 ccgtctctgg ctgacaatac tgagtctgtt cctgacactg tggctgattt cgggattcct
     2881 gccactgtct gcagtaaacc agataatact gaccctggtt tcagttgtgg cggcgggact
     2941 ggtgctgtgg taccagtggt attgtctgtc ttcccgtcgt gatgccccgg agaaggctgg
     3001 cgatgacggt ctgccgcctg aatattttca gggacatgtg ctgcttgttg ccggaaactc
     3061 agatgcgtgg ttcgaacccg gtcaggcata ccgggaaaca gcatcgggat ggtatcttcg
     3121 tgtggatacg ccggagcaga tgagaatact ggcagagcgg ttgtcgtaca gccgtccggc
     3181 cctgtgtggc caggtcgtgg tcatgctggg acttttaccg gagcagcata cctcagatga
     3241 agctttcctg tcgtggttgc ggaactggca gcgtgcagtc atgcagtgcc ggcagtggct
     3301 gaataccatg ccgccggtgc tggtgacatt gatggtttca gggccgtcac cacaaaacac
     3361 atccttaaat aagcactctg cacactggtt tacactgaca ccggacctgt cgggaatgca
     3421 catacgcctg acggaagccg gagccgttcc ggcagaggaa tggctccggg agtgtgatgc
     3481 agcgtccagg ctccggtgcg tcagtgaact gttatggctg aatgccatgt tgtcctggta
     3541 caaccgggtt actgatgccc tgacagatat ttgccggcag gagcactttt atttccggcc
     3601 ggttgccgtt ggattctgtc tgacgtcggt tgcggcccgg cccggtaatc tctggcaaca
     3661 acaactgacg tcactcactg tactgccacc gcctgccggc gtgtcatcac aggcactgcc
     3721 gttgccggat attctgctgt ccggtattcc ccgtcatcgt ggactcagtc actggcacag
     3781 actttgtctc cagacggggg cgattgtcgg cgtttttctt ctgctggcta tgctggcgtc
     3841 ttttatgaat aaccagcgac tggttcgcag cgtgggggaa cacctgacgg cttggcaccg
     3901 gatgcgcgga tattcatctg aatcgaaaaa aactgcactg caacgtcttc aggctgacag
     3961 ccagttactg aacgactggc agcaacgtgg tgtgccgcag cgttacggac tgggattgta
     4021 tcagggaatg tggctgatac cgccggttga gcgagcgact ggtgagtgga ttgcaccact
     4081 gcctccccgt ccggtaatac agaaagttac tcagacgccg aaaatggttc gccttaacag
     4141 cctggcgctg tttgatgccg gtaaatggac gctgaaaccg ggcgccacaa aatggctggt
     4201 gaatgccctg gtggatatta aggcaaaagc gggctggctg attgtggtca gtgg
//
LOCUS       DAZGQL010000202         4225 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.204, whole genome shotgun sequence.
ACCESSION   DAZGQL010000202 DAZGQL010000000
VERSION     DAZGQL010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4225
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.204"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..768
                     /gene="ompT"
                     /locus_tag="ACHKIR_005034"
     CDS             <1..768
                     /gene="ompT"
                     /locus_tag="ACHKIR_005034"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease OmpT"
                     /protein_id="HFO9992636.1"
                     /translation="DWKFNNAAIIKGAINWDLMPQISIGAAGWTTLGSRGGNMVDQDW
                     MDSSNPGTWTDESRHPDTQLNYANEFDLNIKGWFLNEPNYRLGLMAGYQESRYSFTAR
                     GGSYIYSSEEGFRDDIGSFPNGERAIGYKQRFKMPYIGLTGSYRYEDFELGGTFKYSG
                     WVEASDNDEHYDPGKRITYRSKVKDQNYYSVAVNAGYYVTPNAKVYIEGAWNRVTNKK
                     GDTSLYDHNDNTSDYSKNGAGIENYNFITTAGLKYTF"
     gene            1000..1668
                     /locus_tag="ACHKIR_005035"
     CDS             1000..1668
                     /locus_tag="ACHKIR_005035"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO9992637.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFDPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVKGTVVMFSA
                     SGKK"
     gene            complement(1750..2092)
                     /locus_tag="ACHKIR_005036"
                     /pseudo
     CDS             complement(1750..2092)
                     /locus_tag="ACHKIR_005036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416064.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            2105..2700
                     /locus_tag="ACHKIR_005037"
                     /pseudo
     CDS             2105..2700
                     /locus_tag="ACHKIR_005037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415069.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            3184..4137
                     /gene="ompT"
                     /locus_tag="ACHKIR_005038"
     CDS             3184..4137
                     /gene="ompT"
                     /locus_tag="ACHKIR_005038"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease OmpT"
                     /protein_id="HFO9992638.1"
                     /translation="MRAKLLGIVLTTPIAISSFASTETLSFTPDNINADISLGTLSGK
                     TKERVYLAEEGGRKVSQLDWKFNSAAIIKGAINWDLMPQISIGAAGWTTLGSRGGNMV
                     DQDWMDSSNPGTWTDESRHPDTQLNYANEFDLNIKGWLLNEPNYRLGLMAGYQESRYS
                     FTARGGSYIYSSEEGFRDDIGSFPNGERAIGYKQRFKMPYIGLTGSYRYEDFELGGTF
                     KYSGWVESSDNDEHYDPGKRITYRSKVKDQNYYSVAVNAGYYVTPNAKVYVEGAWNRV
                     TNKKGNTSLYDHNNNTSDYSKNGAGIENYNFITTAGLKYTF"
BASE COUNT         1329 a          924 c          898 g         1074 t
ORIGIN      
        1 gactggaaat tcaataacgc tgcaattatt aaaggtgcaa ttaattggga tttgatgccc
       61 cagatatcta tcggggctgc tggctggaca acactcggca gccgaggtgg caatatggtc
      121 gatcaggact ggatggattc cagtaacccc ggaacctgga cggatgaaag tagacaccct
      181 gatacacaac tcaattatgc caacgaattt gatctgaata tcaaaggctg gttcctcaac
      241 gaacccaatt accgcctggg actcatggcc ggatatcagg aaagccgtta tagctttaca
      301 gccagaggtg gttcctatat ctacagttct gaggagggat tcagagatga tatcggctcc
      361 ttcccgaatg gagaaagagc aatcggctac aaacaacgtt ttaaaatgcc ctacattggc
      421 ttgactggaa gttatcgtta tgaagatttt gagctaggtg gcacatttaa atacagcggc
      481 tgggtggaag catctgataa cgatgaacac tatgacccag gaaaaagaat cacttatcgc
      541 agtaaagtca aagaccaaaa ttactattct gttgcagtca atgcaggtta ttacgtaacg
      601 cctaatgcaa aagtttatat tgaaggcgca tggaatcggg ttacgaataa aaaaggtgat
      661 acttcacttt atgatcacaa tgataacact tctgactaca gcaaaaatgg tgcaggcata
      721 gaaaactata acttcatcac tactgctggt cttaagtaca ccttttaaca acgttaaata
      781 gattttctcc ccggtggtat tgtatagccc cggggaattt gtttaccgtt aaaagcaaac
      841 aatatcccct aatcttgtaa gcatttaacc tgcgtcctgg cgtgtaacct cttaccgcaa
      901 cacaagactc aatatattca aattcgttat gccgtggcat catttaaccc cttgtaattc
      961 atcgctataa ttgatttaat tcacaaataa aactataaca tggtgaaact aatgaaaaaa
     1021 aacacagatg atggggctaa aatttacaca ccacttaccc taaagcttta tgactggtgg
     1081 gttttgggtg tatcaaatcg gcttgcatgg ggatgtccta caaaggaaca ccttcttcca
     1141 cactttctgg aacatttagg taacaaccat ctggatattg gcgttggaac tgggttttac
     1201 cttactcacg tacctgagag tagtctgata tctttaatgg atttgaacga agctagcctg
     1261 aacgcggcat cgacaagggc tggggaatca aaaattaaac ataaaattag ccatgatgtt
     1321 tttgatcctt atcccgcggc gttacatggt caatttgatt ccatttccat gttttacctt
     1381 cttcactgcc tgcctggaaa tatatctaca aaaagctgtg taatacgcaa tgcggcgcag
     1441 gccttaactg acgatggaac tctatacgga gccacaattc ttggtgacgg cgttgtgcac
     1501 aatagcttcg gtcaaaaact gatgcgcatt tacaatcaga aaggcatctt ttcaaacaca
     1561 aaagattccg aagaaggctt aacacatata ctctcagagc atttcgagaa tgttaaaacc
     1621 aaggttaaag gtactgtagt aatgttttcc gcttcaggga aaaaatagca tccagccgca
     1681 gcacgttctt gcgcacgacg ttctgcggca taatcccaat gattactctc tgacagggtt
     1741 cacaggccac taaatatcag gggcagttga tgtatcaact cggttcagca acacccgata
     1801 catcttccag gcttccagca acgaggtttc ttcctccgta tcctttatcc atgatatgcc
     1861 gttccacttc tgaaactccc ctgccgggga tacctggtga cattttccgg taacggaccg
     1921 agttcagaaa taaataatgc gtcccctgat gccacgtcat aaaccgtttt accccgatgg
     1981 tcttcaacga gatgccacga tgcctcatca ctgttgaaaa caaccacgaa gccagccgga
     2041 atatctgggc aatatcagta ctgtttgcag gcaaacctgt atgaggtgga atggcacaag
     2101 agacttacaa aagaatcagg ccaaacgcct gattgatgag agccccgaac cactgaaatc
     2161 tgtcgtcaaa tttgcgctgg taactgttct gagaaagtcg aacatcataa atctggagtg
     2221 gcaacagatt gacatgcagc gacaagttgc cagggtgaat ccagaagata gcaaatcaaa
     2281 ctgcgccatt ggtgtggcgc tgaacgatac cgcctctaaa gtgttgcgtg atcaaacagg
     2341 caagcatcac aaatgtgggt gtacatacca gggcggctaa gcgagcagat ggaacatcaa
     2401 tgcctgcgct caggaagatg cgcattgaca gcaagacagc atggctatca gcttgtcgtc
     2461 gtgcaggaat tgaaaatttc cgtttccatg acctctggca tacctgggca agctggttga
     2521 ttccgtcagg cgtcccatta tcagtgcttc aggaaatggg cggatgggag tccagagaaa
     2581 tggttcgtag atatgctcac cttgcgccta atcatttgac tgagcacgcg aggaaaatag
     2641 acgacatttt tggtgataat gtcccactct ggaattatag aagaaataaa gaaggcgtaa
     2701 ctgattgaat tttaatggcg cgccctgcag gattcgaacc tgcggcccac gacttagaag
     2761 ttcctagaac gacattttaa gtcaacaact taccgcgcca tctctgcgct cacacgtccc
     2821 actacctcaa aacatgtaaa gccttgcaag ccattgcgag gccttatgtg tctcagtttt
     2881 gtccctcttt tttgtactaa aaaacatagt aattgaggat aaacctcatg ctattttcgc
     2941 ttatatgcct ctaaaggcat ggcacttaaa tagataaaag caccacaaaa gcataaaaaa
     3001 accacacagt aaaaccgcaa tatgaaacaa taacagataa ttaaaccaaa aacagatagc
     3061 gcattgtgat aatcattcaa tactaaacaa aatataaaca gtggagcaat atgtaattga
     3121 ctcattaagt tagatataaa aaatacatat tcaatcatta aaacgattga atggagaact
     3181 tttatgcggg cgaaacttct gggaatagtc ctgacaaccc ctattgcgat cagctctttt
     3241 gcttctaccg agactttatc gtttactcct gacaacataa atgcggacat tagtcttgga
     3301 actctgagcg gaaaaacaaa agagcgtgtt tatctagccg aagaaggagg ccgaaaagtc
     3361 agtcaactcg actggaaatt caatagcgct gcaattatta aaggtgcaat taattgggat
     3421 ttgatgcccc agatatctat cggggctgct ggctggacaa ctctcggcag ccgaggtggc
     3481 aatatggtcg atcaggactg gatggattcc agtaaccccg gaacctggac ggatgaaagt
     3541 agacaccctg atacacaact caattatgcc aacgaatttg atctgaatat caaaggctgg
     3601 ctcctcaacg aacccaatta ccgcctggga ctcatggccg gatatcagga aagccgttat
     3661 agctttacag ccagaggtgg ttcctatatc tacagttctg aggagggatt cagagatgat
     3721 atcggctcct tcccgaatgg agaaagggca atcggctaca aacaacgttt taaaatgccc
     3781 tacattggct tgactggaag ttatcgttat gaagattttg aactcggtgg cacatttaaa
     3841 tacagcggct gggtggaatc atctgataac gatgaacact atgacccggg aaaaagaatc
     3901 acttatcgca gtaaggtcaa agaccaaaat tactattctg ttgcagtcaa tgcaggttat
     3961 tacgtcacac ctaacgcaaa agtttatgtt gaaggcgcat ggaatcgggt tacgaataaa
     4021 aaaggtaata cttcacttta tgatcacaat aataacactt cagactacag caaaaatgga
     4081 gcaggtatag aaaactataa cttcatcact actgctggtc ttaagtacac attttaagaa
     4141 cgccaactaa aatttccccg aggtgaaaat cgccccgggg aataactagc catttcaatg
     4201 taacaattaa cccttaaaat aaacc
//
LOCUS       DAZGQL010000203         4170 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.205, whole genome shotgun sequence.
ACCESSION   DAZGQL010000203 DAZGQL010000000
VERSION     DAZGQL010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4170
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.205"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..721
                     /gene="ydeO"
                     /locus_tag="ACHKIR_005039"
     CDS             <1..721
                     /gene="ydeO"
                     /locus_tag="ACHKIR_005039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HFO9992639.1"
                     /translation="NANILDKDYAFSDGEILMVDNAVRTHFEPYERHFKEIGFNENTI
                     KKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERNHHNFSELLLFSCLS
                     IFATCKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCLYISESLLKKKLKQE
                     QTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYAFRKHFGNSPKRVSK
                     EYRCQRHTGMNTDNTMSALAI"
     gene            1123..2805
                     /gene="ydeN"
                     /locus_tag="ACHKIR_005040"
     CDS             1123..2805
                     /gene="ydeN"
                     /locus_tag="ACHKIR_005040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310130.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO9992640.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTYSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPEQYQKQFNTGSQTADNYYASVYSVDQGVKRILEKLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGEPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDNSQPPLSEVNQEKFNNIKKALSEAK"
     gene            2857..4014
                     /gene="ydeM"
                     /locus_tag="ACHKIR_005041"
     CDS             2857..4014
                     /gene="ydeM"
                     /locus_tag="ACHKIR_005041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFO9992641.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIAASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQIEFNTLTVI
                     NNINVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHTENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCVYKPICNGGCPKHRIT
                     KVNNETISYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(3980..4102)
                     /locus_tag="ACHKIR_005042"
     CDS             complement(3980..4102)
                     /locus_tag="ACHKIR_005042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992642.1"
                     /translation="MTKHPTGIYVGCFVKVIRRRLRLEQRRSVINYSPFVLQHP"
BASE COUNT         1273 a          872 c          833 g         1192 t
ORIGIN      
        1 caacgctaac attttagata aagattacgc cttctctgac ggcgagatcc tgatggtaga
       61 taacgctgtt cgtacgcatt ttgaacctta tgagcggcat ttcaaagaaa tcggatttaa
      121 tgaaaatacc attaaaaaat atctacaatg cactaacatc cagacagtga cggtgcctgt
      181 tcctgcgaag tttttacgtg cttcaaatgt accgactgga ttgcttaatg aaatgattgc
      241 ttatctcaac tcggaagaac gcaatcatca taatttttca gaacttttgc ttttttcttg
      301 cctgtctatt tttgccacat gcaaaggttt cattacacta ttaactaacg gtgtgctatc
      361 cgtttccggg aaagtgagaa atattgtcaa catgaagctg gcgcacccat ggaagctgaa
      421 agatatttgt gactgcctgt acattagtga aagcctgttg aagaaaaaac taaagcaaga
      481 gcaaacgaca ttctcacaga ttcttttaga tgccagaatg cagcacgcaa aaaatttgat
      541 acgcgtagaa ggttcagtca ataaaattgc cgaacaatgt ggttatgcga gtacatctta
      601 ttttatttat gcgttccgca aacatttcgg caacagtccg aagagagttt ctaaggagta
      661 ccgttgtcaa aggcacacgg gtatgaacac ggataacacg atgagtgctt tagctatttg
      721 attatttgct aacgagtagt caaccacgca cgttacgtag aaattaaaat ggggcagcca
      781 ttccctgccc cgcgttgttt ttaggcgata tattattgaa ataaataagt gacattcatc
      841 acatatttat gcatttgcat aacctgttgc atgattattt atgatctcaa ttcggaattt
      901 tgtcagtaaa atacaataat ttattaaata tcaatagatt agttgtttat ctgcgagaaa
      961 ttacttagta gaatagaaag taatgtcaac agtttatgga ctgttatttc tcttttttta
     1021 gcttaatctg ctatctcttt atgtgactaa cttcacttac atccacttat ttctcttcgt
     1081 aaaattactt tggaattaag tacaataaga agaggaacat ttatgaagtc tgcattaaag
     1141 aaaagtgtcg taagtacctc gatatctttg atactggcat ctggtatggc tgcatttgct
     1201 gctcatgcgg cagatgatgt aaagctgaaa gcaaccaaaa caaacgttgc tttctcagac
     1261 tttacgccga ctgaatacag taccaaggga aagccaaata ttatcgtact gactatggat
     1321 gatcttggtt atggacaact tccttttgat aagggatctt ttgacccaaa aacaatggaa
     1381 aatcgtgaag ttgtcgatac ctacaaaata gggatagata aagccattga agctgcgcaa
     1441 aaatcaacgc cgacgctcct gtcattaatg gatgaaggcg tacgttttac taacggctat
     1501 gtggcgcacg gtgtttccgg cccctcccgc gccgcaataa tgaccggtcg agcgcccgcc
     1561 cgctttggtg tctattccaa taccgatgct caggatggta ttccgctaac agaaactttc
     1621 ctgcctgaat tattccagaa tcatggttat tacactgcag cagtaggtaa atggcacttg
     1681 tcaaaaatca gtaatgtgcc ggtaccggaa gataaacaaa cgcgtgacta tcatgacaac
     1741 ttcaccacct attctgcgga agaatggcaa ccgcaaaacc gtggctttga ttactttatg
     1801 ggattccacg ctgcaggaac ggcatattac aactcccctt cactgttcaa aaaccgtgaa
     1861 cgcgtccccg caaaaggtta tatcagcgat cagttaaccg atgaggcaat tggcgttgtt
     1921 gatcgtgcca aaacacttga ccagcctttt atgctttacc tggcttataa tgctccgcat
     1981 ctgccaaatg ataatcctgc accggagcaa tatcagaagc aatttaatac cggtagtcaa
     2041 acagcagata actactacgc ttccgtttat tctgttgatc agggtgtaaa acgcattctc
     2101 gaaaaactga agaaaaacgg acagtatgac aatactatta ttctctttac ctccgataat
     2161 ggtgcggtta tcgatggtcc tctgccgctg aacggggcgc aaaaaggcta taagagtcag
     2221 acctatcctg gcggtactca caccccaatg tttatgtggt ggaaaggaaa acttcaaccc
     2281 ggtaattatg acaagctgat ttccgcaatg gatttctacc cgacagctct tgatgcagcc
     2341 gatatcagca ttccaaaaga ccttaagctg gatggcgttt ccttgctgcc ctggttgcaa
     2401 gataagaaac aaggcgagcc acataaaaat ctgacctgga taacctctta ttctcactgg
     2461 tttgatgagg aaaatattcc attctgggat aattaccaca aatttgtccg ccatcagtca
     2521 gacgattacc cgcataaccc caacaccgag gacttaagcc aattctctta tacggtgaga
     2581 aataacgatt attcgcttgt ctatacagta gaaaataatc agttaggtct ctacaaactg
     2641 acggatctac agcaaaaaga taaccttgcc gccgccaatc cgcaggtcgt taaagagatg
     2701 caaggcgtgg taagagagtt tatcgacaac agccagccac cgcttagcga ggtaaatcag
     2761 gagaagttta acaatatcaa gaaagcacta agcgaagcga aataactaaa ccttcatgcg
     2821 gcggattttt ccgccgcctt attgagcgag atagcgatgc acgttacagc caagccctcc
     2881 agttttcaat gtaatctcaa atgtgattac tgtttttacc ttgaaaaaga gtcgcagttt
     2941 actcatgaaa aatggatgga tgacagcact ctgaaagagt tcatcaaaca atatatcgca
     3001 gcgtctggca atcaggtcta ttttacctgg caaggcggtg aacccactct ggctggcctg
     3061 gattttttcc gtaaagttat tcactatcaa caacgctatg caggccaaaa acgtattttt
     3121 aatgcattac aaacgaatgg cattttattg aataatgaat ggtgtgcctt tctcaaagaa
     3181 catgagtttc tggttggtat ctcgatcgat ggcccccaag agctacatga ccgttacaga
     3241 cgcagtaatt caggtaacgg tacttttgca aaagtgatag cagccatcga gcgtctgaaa
     3301 tcatatcaaa tagagtttaa tacgttaacc gttattaata acattaatgt ccattaccct
     3361 cttgaggttt atcatttttt aaaatctatc ggcagtaaac atatgcaatt tatcgaattg
     3421 ctcgaaaccg ggacgccgaa tattgatttc agtggtcata ctgagaatac attccgtatc
     3481 attgattttt ctgtgcctcc cacggcttat ggcaagttta tgtcaaccat ttttatgcaa
     3541 tgggttaaaa acgatgtggg tgaaattttc atccgtcagt ttgaaagctt tgtcagccgt
     3601 tttttgggga atgggcatac cagttgtatt ttccaggagt cctgcaagga taatctggtt
     3661 gttgaaagta atggagacat ttacgaatgc gaccattttg tctatccaca gtacaaaatt
     3721 ggaaacatta ataaatcgga actcaaaacg atgaacagtg tacaactgac agcgcaaaaa
     3781 aaacggattt cagcgaaatg tcagcaatgt gtatataaac ctatctgcaa tggtggttgc
     3841 cctaagcatc gtattactaa agtaaacaat gagactattt cctatttttg cgaaggttat
     3901 aaaatccttt tttcaaccat ggtaccttat atgaacgcca tggtggagtt agccaagaac
     3961 agagtaccgc tttaccacat tatggatgtt gcaaaacaaa tggagaataa ttaataacgc
     4021 tccttctttg ctccagtctt agcctccgtc ttatgacctt aacaaagcac ccgacataaa
     4081 ttcctgttgg gtgtttcgtc atcatcatct tcccttgcga aaattctggc ttacgcggca
     4141 tgcgtcaggg cattagcaat acccttgatt
//
LOCUS       DAZGQL010000204         4168 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.206, whole genome shotgun sequence.
ACCESSION   DAZGQL010000204 DAZGQL010000000
VERSION     DAZGQL010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4168
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.206"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(396..740)
                     /gene="yoaB"
                     /locus_tag="ACHKIR_005043"
     CDS             complement(396..740)
                     /gene="yoaB"
                     /locus_tag="ACHKIR_005043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFO9992643.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            872..2782
                     /gene="yoaA"
                     /locus_tag="ACHKIR_005044"
     CDS             872..2782
                     /gene="yoaA"
                     /locus_tag="ACHKIR_005044"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFO9992644.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            2840..3535
                     /gene="tsaB"
                     /locus_tag="ACHKIR_005045"
     CDS             2840..3535
                     /gene="tsaB"
                     /locus_tag="ACHKIR_005045"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFO9992645.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGADLPMIGVSTLMTMAQGA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDEKGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLTLCDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            3574..4155
                     /gene="yeaY"
                     /locus_tag="ACHKIR_005046"
     CDS             3574..4155
                     /gene="yeaY"
                     /locus_tag="ACHKIR_005046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HFO9992646.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
BASE COUNT          998 a         1034 c         1141 g          995 t
ORIGIN      
        1 tatcgataag gctatatatg accttaattt gtatctaata tcaaagggat tataacgata
       61 tggcacgagt taacctgttg acattcaggt gttttaaagg tagcagctca tagtgtgtta
      121 tccagtaaga tggagaaaat taatggtcag tcctcacgct ataaccagat tgttcaggta
      181 agaaagaaaa ccagagatgc taatcgaagg gcgaacataa cggcatgatt atgtttattt
      241 atatagttag ttttatagat aaaattgaaa ttataaaaat aaataatgaa ttagcaggag
      301 catcaactgt aatatcttaa tctctggtcg atttatgcaa aaccgaccag tattgtcgat
      361 aaattatcgt ctgacttatt ttgcttcgat aaagcttaca ccgcagccac aatcttaatt
      421 tcaactttat acttcgggtt catcaaaccc gcttgtaccg tgcagcgcac cggcgcatga
      481 cccgcgacaa cccaggcatc ccacgcttta ttcatcgccg cgaagtcgtt tttatcggcc
      541 aggaaaatgg tggcatccag aatgctcgat ttattgctgc cctgtttttc cagcacggcg
      601 tcaatctgcg ccagcgtgtt ggcggtttgc tcaaaggcat cggcgtcgag gttttccggt
      661 acaccagtgt agtagagcgt gttgttgtgg attactacat cagaccagcg ggcttcagca
      721 tcgatacgaa cgatagtcat cattttgtcc tcattatact tccattttcg tgctgcaaag
      781 actgccacaa tgtgaggtcg ccgtcaccac ttcaactggc gaaagcgccc tcatcctgac
      841 ataatccctg ttcaaatcaa cagggggtag tgtgacggac gattttgcac cagacggtca
      901 gctggcgaaa gcgataccag gctttaagcc gcgagaacca cagcgacaga tggcggtagc
      961 cgtcacccag gcgatagaaa aaggccagcc gctggtggtg gaagccggaa ccggtacggg
     1021 caaaacctac gcttacctgg cccctgcgct gcgggcgaaa aagaaagtca ttatctcgac
     1081 cggctcaaaa gcgttgcagg atcagctcta cagccgcgat ttgccaacgg tttcaaaggc
     1141 gttgaaatat acgggcaacg tggcgttgct gaaagggcgc tcaaactacc tctgcctcga
     1201 acgtctcgaa cagcaggcgc tggcgggggg cgatctgccg gtacaaatct taagcgatgt
     1261 gatcctgctg cgctcctggt ctaatcaaac agtcgatggt gatatcagca cctgcgtcag
     1321 cgtggcggaa gattcacagg cgtggccgtt ggtcaccagc accaacgata actgccttgg
     1381 cagcgactgc ccgatgtata aagattgctt tgtggtcaaa gcacgcaaaa aagcgatgga
     1441 cgccgatgtg gtggtggtaa accatcatct ctttctggcg gatatggtgg tgaaagagag
     1501 tggatttggc gaactgatcc cggaagctga cgtcatgatc ttcgacgaag cccaccagct
     1561 gcctgacatt gccagccagt attttggtca gtcactctcc agtcgacaac tactcgacct
     1621 ggcaaaagac atcaccatcg cctatcgcac cgaattaaaa gatactcagc agttacaaaa
     1681 gtgcgccgac cgccttgccc agagcgcgca ggattttcgt ctgcaactcg gtgagcctgg
     1741 ttatcgtggc aacctgcgcg aactgttagc taatccgcaa attcaacggg cgtttttact
     1801 gctcgatgac accctggaac tttgttatga cgtggcgaaa ctgtcgctgg ggcgttccgc
     1861 cttgctggat gcggcatttg agcgcgccac gttgtatcgt acgcggctga aacggctaaa
     1921 agagatcaat cagccgggct acagctactg gtacgaatgc acttcgcgcc attttactct
     1981 ggcgctcacg ccgctcagcg tggcggataa attcaaagag ttaatggcgc aaaaacccgg
     2041 tagctggatc tttacctcag caacgctgtc ggtgaacgac gatctgcatc attttacctc
     2101 gcggcttggc attgaacagg cagagtcgtt gctgttaccc agcccgtttg attacagccg
     2161 ccaggcgtta ctctgtgtgc cgcgcaacct gccgcaaacc aatcaaccgg gctccgcacg
     2221 gcaactggcg gcaatgttgc gaccgatcat cgaagctaac aacggtcgtt gttttatgct
     2281 ttgtacctcg cacgccatga tgcgcgatct ggctgagcag ttccgcgcta ccatgacgct
     2341 tcccgttttg ttgcaggggg aaaccagcaa agggcaactg ttgcagcaat ttgtcagcgc
     2401 cggtaacgcg cttcttgtgg caaccagcag cttctgggaa ggggtggatg tgcgtggcga
     2461 tacattgtca ttggtgatta tcgacaagtt gccgtttacc tcaccggatg atccactatt
     2521 aaaagcgcgc atggaagatt gccgtttgcg tggtggtgac ccgttcgatg aagtacaact
     2581 gccggatgcg gtgattactc tcaagcaggg agtagggcga ctgattcgcg acgccgacga
     2641 tcgcggcgtg ctggtgattt gcgataatcg gctggtgatg cgcccttacg gcgcgacgtt
     2701 tctcgccagt ctgccgccag cgccgcgcac ccgtgacatt gcccgtgcgg ttcgtttcct
     2761 tgcgatacca tcctccaggt aatttgttac ggagtatggt atgatgcgcg cctttttatt
     2821 gatctaagca cgaagaccca tgcgaattct ggctatcgat accgcgacag aggcctgctc
     2881 tgtcgccctg tggaacgacg gtactgtcaa cgctcatttt gagctttgcc ctcgtgaaca
     2941 tactcaacga atcttaccga tggtgcagga tatcctgacc accagcggaa cttccctgac
     3001 tgatattaac gctctggctt acgggcgcgg ccccggtagc tttaccggcg tgcgcattgg
     3061 tattggtatt gcgcaggggc tggcgcttgg cgcggatctg ccgatgattg gcgtttccac
     3121 gctcatgaca atggcgcaag gggcgtggcg caaaaacggc gcaacccgcg tgctggctgc
     3181 tattgatgcg cggatgggcg aagtttactg ggccgaatat cagcgtgatg aaaagggtat
     3241 ctggcacggt gaagaaaccg aagccgtact caaacccgaa ctcgttcatg aacggatgca
     3301 acagctttcc ggggaatggg tgacggtagg tacaggctgg caagcctggc cggatctcgg
     3361 taaagagagc ggtttgaccc tgtgcgatgg cgaagtgtta ctgcccgctg cagaagatat
     3421 gcttccgatt gcgtgtcaga tgtttgccga gggtaaaacg gtggcggtgg aacatgccga
     3481 accggtttat ttacgtaaca acgtcgcatg gaagaaactt ccgggcaaag aatgaatctt
     3541 aagagtatgc cctgagaaaa aaggagtcgc atcatggcgg ttcaaaagaa tgttatcaaa
     3601 ggcatactgg caggtacgtt tgcgctaatg ctcagcggtt gtgttactgt gccggacgcc
     3661 attaaaggca gcagtcccac gccgcaacaa gatttagttc gggtgatgag tgcgccgcag
     3721 ctgtacgttg gtcaggaggc acgctttggt ggcaaagtgg ttgcggtaca aaaccagcaa
     3781 gggaaaaccc gcctggaaat tgccaccgta ccactggaca gcggagccag accgacgctg
     3841 ggagaacctt ctcgcggtcg catttatgct gatgtgaacg gttttctgga cccggtagat
     3901 ttccgtggac aactggttac ggtagttggg ccaatcaccg gtgcggttga cggcaaaatc
     3961 ggcaacacgc cctataaatt tatggtgatg caggtaacgg gttacaaacg ttggcattta
     4021 acccagcagg tgattatgcc gcctcagccg attgatccat ggttttatgg cggtcgtggc
     4081 tggccctatg gctacggtgg atggggctgg tataatcccg gccccgcgag agtacaaact
     4141 gttgtaactg aataattgct tgttttta
//
LOCUS       DAZGQL010000205         4057 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.207, whole genome shotgun sequence.
ACCESSION   DAZGQL010000205 DAZGQL010000000
VERSION     DAZGQL010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4057
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.207"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>4057
                     /locus_tag="ACHKIR_005047"
     CDS             <1..>4057
                     /locus_tag="ACHKIR_005047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD38 domain-containing protein"
                     /protein_id="HFO9992647.1"
                     /translation="LDNYFGRYDVSTDGKITSGGDFMTEAQRARRVFKDGRYVDAEYP
                     VRQEVYDAFKGVIQAIKNSDMPRRSALLDEVRSKPYWSTDVEMAARAFERYVQDKARM
                     AGVENDYLVNIRKAPEHNTDNTYAYPTNAELDGGIREAFDHLFRTLKTRETDKGVAFY
                     SRKGVTRTPEGNLISDVNRSAEAKGSPVPQVEAVARGVISGIKDSDLKVRVVKSQKEA
                     EALAGESFDGYGKVHAFYRPDKREIVLVADNIPDGRTVREKLRHEIIHHAMEHVVTPA
                     EYQTIIKTVLKTRDSDNATIREAWRKVDASYGKESPEVQAGEFLAHMAEKQPNKFVAA
                     WERVVALVKGVLRRTGLLKPTELNDIRLVRETIRTLGQRVREGYTPREDGVGASSQYS
                     RSGKPDPFKVPEGEGERYRDDLARMMKSLRTTDLTVNIGRTPPVLRHLGAPDLPLVIS
                     RDTVRKATNGVKHVVPMDVIERLPELMHDPDAIYRSATERNAVVMLLDAVDKNGDPVV
                     SAVHMKAVRSRLEINKVASVYGTENGKKLKSMEMTGLTLYRREKLSRDNLLHRGLQLP
                     KGEHSYRGSADKILYPEDIRKGPYYSRTSSLTPEETIASRFVRQMQDKFQVLKAVQEN
                     IRKTGGKVDDSNNAYMAEELFHGKAENDLNVMKERYVQPLAKLLADYKIAQADLDEYL
                     YARHAPERNAHIAKINPKMPDGGSGMTNAEAAEIMQRVRNSGKQAQYDRLAGIIDDML
                     ARRRELIREAGLEENGVVDAWQNAYRYYVPLKGQDVDGVVSLPRTGKGFTIGGRESRQ
                     AMGRASRAQSPSTQAIQDLSESLIRHRKNEVGNAFLKLVQDNPDKDYWQVFTDDRPDT
                     MRVIAERKDQETGETIREAVERPVAMAMMADRYFTTKKNGKTYYIKLHDPRLMRAMKN
                     MGPETSNAVIRTLGKVNRFLATVNTSYNPEFLVSNFIRDVQTAVMNLKAEQGRSDGKL
                     KGLDNLSALAVVKDSRSAMSAVYASLRGKTLTGNGAQWQKVWKEFVEDGGKTGWFNMG
                     DLEGQQKEMDRLVSLAKGGWKGQSIGAWNSFLNLVEDANGAVENALRLSAYKHARDAG
                     LSRQQAASLAKNMTVNFNRRGEQGALMNSLYMFANASIQGTANLVRTLGHLNGDGPLL
                     ERLRWKNLNVPQKIALAAVGAGYLLGSLNRSVAGEDDDGVNWYDKVPSHVKERNLVIM
                     KSVFGGKAGEYWSIPLPYGYNVFFLLGHTAEGVAAGDLTASRAAGNVVGGVLGAFSPV
                     GSETSETLSGALLKNAAPTILRPFANLAMNENFMGAQIYQENMPFGTPKPDSQLGRRS
                     TPEAYKAFASWLNAFSGGSQYRPGAVDVTPES"
BASE COUNT         1008 a          954 c         1233 g          862 t
ORIGIN      
        1 ctggataatt attttggtcg ttatgacgtt tccactgacg ggaagattac gtcaggtggc
       61 gactttatga cggaagcaca gcgtgccagg cgcgtattta aagacggcag gtatgttgat
      121 gctgaatatc cggtacgtca ggaggtttac gacgctttta aaggtgtgat tcaggccatt
      181 aaaaacagtg acatgccgcg tcgttcagcg cttctcgatg aggtgcgctc aaaaccgtac
      241 tggtcaacgg atgttgaaat ggcggcacgt gcctttgagc gttatgttca ggataaggcg
      301 cgtatggctg gcgtggagaa tgattatctg gtcaatatcc gtaaggcacc tgagcacaac
      361 acagataaca cctacgctta tccgacgaat gcggaactgg atggcggtat tcgtgaggca
      421 ttcgatcacc tgttccgcac cctgaaaacc cgtgagacgg acaagggagt tgcgttttat
      481 tcccgtaagg gcgtgacccg cacacctgaa ggcaatctga tttcggatgt taaccgcagt
      541 gcggaagcca aaggcagtcc ggtcccgcag gttgaagctg ttgcccgtgg cgtgattagt
      601 ggcattaagg acagtgacct gaaggtccgc gtggtgaagt cacagaaaga ggctgaagca
      661 ctggcgggtg aatcattcga cggttacggc aaggtgcacg cgttctatcg cccggataaa
      721 cgagaaattg tcctggtggc ggataacatc cctgacgggc ggaccgttcg cgagaagtta
      781 cgtcacgaga ttattcacca tgcaatggag catgtagtca cgccagcgga atatcagacg
      841 attattaaga ccgtgctgaa aacccgtgac agtgataacg ccaccatccg tgaagcctgg
      901 cgtaaggttg atgcgtctta tggtaaggaa tcaccggaag tgcaggcggg tgaatttctg
      961 gcgcatatgg cagagaagca gccgaataaa ttcgtggcgg catgggagcg tgttgttgcc
     1021 ctggtcaaag gggtactgcg tcgtacgggg ttactgaagc cgacggaact gaacgatatc
     1081 agacttgttc gcgaaactat tcgcacgtta ggccagcgtg tgcgggaagg ttacacgccg
     1141 cgtgaggatg gcgtgggcgc atcgtctcag tactcccgta gtggtaagcc tgatccgttc
     1201 aaagtgccgg agggtgaggg tgaacgttat cgcgatgacc ttgccagaat gatgaaatct
     1261 ctgcgtacca cagatttaac ggtaaacatc gggcgtacgc cgccggtatt gcgtcacctt
     1321 ggcgcaccgg atttgccgct ggttatttcc cgcgatactg tgcggaaggc caccaatggt
     1381 gtgaaacatg tggtgccgat ggatgttatc gagagactac cggaactgat gcacgatccg
     1441 gatgcaattt accgctcagc gacagaaaga aatgcggttg tgatgctgct tgatgccgtg
     1501 gataaaaatg gtgatccggt ggtgtcagcg gtacacatga aggctgtccg gtcgcgtctg
     1561 gaaatcaaca aggtagcttc tgtttacggt acagaaaatg gaaaaaaact gaagagtatg
     1621 gaaatgaccg ggttaacgtt gtaccggaga gaaaaattaa gccgcgataa ccttctgcac
     1681 agagggctcc aattgcccaa aggggaacat tcttatcgcg gctctgcgga taaaatactc
     1741 tatcctgaag atattcgcaa ggggccgtat tactcccgta ccagcagtct gacaccggaa
     1801 gagacaattg catcgcgttt tgtgcgccag atgcaggata aattccaggt gctgaaagct
     1861 gttcaggaga atatccgtaa aactggcggc aaagtggacg acagtaacaa cgcttatatg
     1921 gcggaagaac tcttccacgg gaaggcggaa aacgacctga acgtgatgaa ggagcgctac
     1981 gttcagccac tggctaaatt actggcggac tacaaaattg cgcaggccga tctggatgag
     2041 tacctctacg cccgtcacgc gccggaacgt aacgcgcata tcgcgaaaat caacccgaaa
     2101 atgccggacg gcggttcggg gatgaccaac gcggaagcgg cggaaatcat gcagcgtgta
     2161 cgtaacagtg gcaaacaggc acagtatgac cgtctggcag ggattattga cgatatgctg
     2221 gcccgtcgcc gtgagcttat ccgtgaggcc ggacttgaag agaacggtgt ggtggatgcc
     2281 tggcagaacg cctaccgtta ctacgttccc ctgaaaggtc aggatgttga cggtgtggtg
     2341 tcactgcccc gtacaggtaa gggcttcacc atcggcggac gtgaaagcag gcaggccatg
     2401 gggcgtgcat cccgggcaca gtctccgtcc actcaggcga tacaggacct gagcgaatcg
     2461 ctgatccgcc atcgcaaaaa tgaagtgggt aacgccttcc tgaagctggt gcaggataat
     2521 cccgataagg attactggca ggtattcact gatgacagac cggataccat gcgtgtgatt
     2581 gcagagcgca aggaccagga aactggtgaa accattcgcg aagctgtcga gcgtccggtg
     2641 gcgatggcaa tgatggcaga ccggtacttc accaccaaaa agaacggcaa aacgtactac
     2701 atcaaactcc atgatccgcg cctgatgcgt gcgatgaaga atatgggacc ggaaaccagt
     2761 aacgccgtaa tccgcacgct ggggaaagtt aaccgcttcc tggcaacggt gaacacgtct
     2821 tataacccgg aattcctggt cagtaacttc atccgtgacg ttcagacggc ggtgatgaac
     2881 ctgaaggcgg agcagggaag gagcgacggc aaactgaaag ggctggataa cttatccgca
     2941 ctggctgtgg tgaaagacag ccggtctgcc atgtctgccg tatacgccag tctgcgtggt
     3001 aaaaccctca cgggcaacgg tgcgcagtgg cagaaggtgt ggaaagagtt tgttgaggac
     3061 ggaggtaaaa ccggctggtt taacatgggt gaccttgaag gccagcagaa ggaaatggat
     3121 cgccttgtat cgctggcgaa gggaggatgg aaaggccaga gtatcggtgc atggaattcg
     3181 ttccttaacc ttgtcgagga tgccaacggg gcggttgaaa acgctctgcg tctttctgcc
     3241 tataaacacg cccgtgatgc cggtttgtca cgccagcagg cggcgtctct tgccaaaaac
     3301 atgacggtga actttaaccg tcgtggtgag cagggagcgc tgatgaactc gctgtacatg
     3361 ttcgccaacg ccagcattca ggggaccgca aacctggtga gaacgctcgg acatcttaat
     3421 ggcgacgggc cgttactgga gcgccttcgc tggaagaatc tcaatgtacc gcagaaaatc
     3481 gcgcttgcag ctgtgggagc gggttatctg cttggctcgc ttaaccgcag tgttgcgggg
     3541 gaggatgatg acggggttaa ctggtatgac aaggtgccgt ctcatgtgaa agagcgtaac
     3601 ctcgtcatta tgaaatcggt gttcgggggc aaggccggag agtactggag tattcctctg
     3661 ccttacgggt acaacgtttt cttcctgctt ggtcacacag ccgaaggggt ggcagcgggc
     3721 gacctgaccg cttcacgtgc tgccggtaat gttgttggtg gtgtgcttgg ggcattcagt
     3781 cctgtgggca gtgaaacgtc ggaaacactg tccggggcat tgctgaaaaa tgcagcgccg
     3841 accattctgc gtccgtttgc gaaccttgcc atgaatgaaa acttcatggg ggcgcagatt
     3901 taccaggaga acatgccgtt tggtacacca aaacctgaca gtcagctggg aagacgttca
     3961 acgccagaag cgtacaaggc gtttgcatcc tggctgaatg cgttctcagg tggcagccag
     4021 taccgtcccg gcgcggtgga tgttacgccg gaatcac
//
LOCUS       DAZGQL010000206         4055 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.208, whole genome shotgun sequence.
ACCESSION   DAZGQL010000206 DAZGQL010000000
VERSION     DAZGQL010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4055
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.208"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..546
                     /locus_tag="ACHKIR_005048"
     CDS             <1..546
                     /locus_tag="ACHKIR_005048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2345 domain-containing protein"
                     /protein_id="HFO9992648.1"
                     /translation="IRFVTDKIRELQSAVLLGSAPQGVALTSGEHLQLSSTRNTMINA
                     GQHLDIGAMKNLSVTVEKALGLFVHKDGAKLVANQGDIEIQAQHNTMALFSEKQLTVT
                     SSEDEIIISTPETLTLNGGGSYLRLSKNGIEHGSSGEFIVKTSDYLVPGSGANLPNEA
                     PGFSLTDITQESKISSKSFND"
     gene            562..2790
                     /locus_tag="ACHKIR_005049"
     CDS             562..2790
                     /locus_tag="ACHKIR_005049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 104 protein"
                     /protein_id="HFO9992649.1"
                     /translation="MSVLPKIVWPIPSSRRGSEFKNQEEILSHLGGESTGLYMIGRSG
                     MWHGGIHITEATTPWCALSGKSPLEAIDFPLPFKGEQAIRCMADGEVIAYRICRDYLT
                     LGWESGPLSFSGSFVLVKHYIQPGEKETSGLHFYTLYMHLAPYSAYESEAENQWVVQD
                     TLKAYSEMDWLTAKLTSDETSPQIAGYMPEGARVEWDPADSNLNATGSNKRKYGLVTL
                     KGLPEKTSSTLTPGQRYWVVVDNNNVKPAAGAGPGWWRHLLPPAKEAMVFDKTVSLRS
                     PFAIKAGDPIGHMGYYQAPKDGGYEARYQVHIECTSMDDNLEKFLTNPEQVGKNNPLW
                     LKYAPGLALYKKDVSTGTFTKDTGVTVRTGILPLKQVQTEVDKSTKQEYWQLRPENAY
                     VPKGQVEPQLLSQYDLAKLGFRTEIAEPTSFDYLDGKKQPTGFFRSLINSLYEAAIDD
                     SRTSHALVKHNYQRLLDKIDSGSDRYSPMEYWRALHNPDYRDVLQKTIVKHPSDWYFK
                     KDDAIWQPFLNALKKDAPEWKKYSEDFLDKMAWMQDVTTEKLGPSLWHMHPIMFLASL
                     IQPNEDIRILRLRAFLRMIRIGEGTIQEDGYRTMFTGVKFTDFSKHPNIRHEANGVVS
                     TAAGAYQFLYGTWRNLQRRYSFPDFSPSNQDLGCIALIAGRKALDVVMQGKISEAIHL
                     CRIEWASLPGSPHAQPTANKKMIMEKYELYLAEEKEGKTSLHATTEKIVKFIEGNYPE
                     YL"
     gene            2787..3230
                     /locus_tag="ACHKIR_005050"
     CDS             2787..3230
                     /locus_tag="ACHKIR_005050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992650.1"
                     /translation="MKKFIFLLLIPVFCNANTPTVFKSSCSGIQYSIESFCSVNNLND
                     DQDKLTIPICSKQIFTLGKENHDLSESTGFVERINIKGEKIKMMNFVFYGATCKSDRI
                     ILSGAGGCNSCGEMFKIFNLSGKEINEKSTMLDLEPMQDIIPDFY"
     gene            3857..3988
                     /locus_tag="ACHKIR_005051"
     CDS             3857..3988
                     /locus_tag="ACHKIR_005051"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992651.1"
                     /translation="MDSENKIQVHKSIDYCIGVVGINNDDVKVKISIQDGNDSKNSE"
BASE COUNT         1255 a          766 c          941 g         1093 t
ORIGIN      
        1 attaggtttg taaccgataa aatcagggaa cttcagtctg cggtcctgct gggtagcgcg
       61 ccacaggggg tggccctgac ctcaggtgaa caccttcagc tcagcagtac ccgtaatacc
      121 atgatcaatg caggccagca tcttgatatc ggtgcgatga aaaatctgtc ggtaacggtg
      181 gaaaaagccc ttggtctgtt tgtgcataaa gacggggcga agctggtagc caatcaggga
      241 gatattgaga ttcaggctca gcacaacacg atggcgctgt tttcagaaaa acagctgacg
      301 gtgaccagta gtgaagacga aattattatc agcaccccgg aaaccctgac gctgaacggt
      361 ggcgggtctt acctgagact gagtaagaac gggatagagc acggctcatc gggtgagttt
      421 attgtgaaga cgtctgacta tctggtgcct ggttctggcg caaatctccc caacgaagca
      481 cctggtttca gcctgacgga tatcacacag gaaagcaaaa tcagcagcaa gtcttttaac
      541 gactgacagg gagtatgaat cgtgtctgta ttacccaaaa tcgtctggcc gatcccctcc
      601 agccgtcggg gaagtgagtt caaaaatcag gaagagatcc tctctcattt gggaggagaa
      661 tctaccgggc tgtacatgat aggccgtagt ggcatgtggc atggtgggat ccatatcacc
      721 gaagccacga caccatggtg tgcgcttagc ggtaagtctc cacttgaagc gatagatttt
      781 ccgcttcctt ttaaaggtga gcaggcaatc cgatgcatgg ctgacggaga agtgattgct
      841 taccgcatct gcagggatta tctgacgcta ggctgggaat caggtccgct aagtttttcg
      901 ggctcatttg ttctggtgaa acactatatc cagccgggtg agaaagaaac cagcggactg
      961 catttctata ctctgtatat gcatcttgcg ccgtattcag cttatgaatc tgaagcagaa
     1021 aatcagtggg tcgttcagga cactctaaaa gcatattctg aaatggactg gctgacggcg
     1081 aaactgactt cagatgaaac ttctccacaa attgcagggt acatgcctga aggggccaga
     1141 gtggagtggg acccggcaga cagcaatctt aacgctacag gaagtaacaa gagaaaatac
     1201 ggtcttgtga cgctaaaggg tttaccagaa aaaaccagca gtactctcac tcccggtcag
     1261 cgctactggg ttgttgtgga taacaacaat gttaagccag cggcgggggc cgggcccggc
     1321 tggtggcgac acttgctgcc tcccgcaaag gaagccatgg tattcgataa aacagtaagc
     1381 ctccgttccc cattcgccat taaggctggt gacccgattg ggcacatggg ctactatcag
     1441 gcaccaaagg atggggggta tgaggcacga tatcaggtac atatcgaatg cacgagtatg
     1501 gatgataatc tggagaaatt cctgaccaat cctgagcagg tgggaaaaaa taatccactc
     1561 tggctgaagt acgccccggg acttgcgttg tacaagaaag atgtttctac aggaacattc
     1621 acgaaggata cgggggtgac ggtcaggacg ggaattctgc cactgaaaca ggtgcaaaca
     1681 gaagtcgata agtcgaccaa acaggaatac tggcaattgc ggccagaaaa cgcgtacgtg
     1741 ccaaaaggtc aggtggaacc tcaacttttg tcacagtatg atctggcaaa gctggggttc
     1801 agaactgaaa tcgcagagcc taccagcttt gattacctgg atggaaaaaa acagcccact
     1861 ggattcttcc gcagtcttat caactcactc tatgaggctg cgatcgatga ttcgcgaacc
     1921 agtcatgcgc tggtgaagca taattatcag cgcctgctgg ataaaattga tagtggaagc
     1981 gatcgttact ctccaatgga atactggaga gcacttcata atcccgacta tcgtgacgtt
     2041 cttcagaaaa caatcgttaa acatcccagc gactggtact tcaaaaaaga cgatgcaatc
     2101 tggcaaccat ttcttaatgc gctgaaaaaa gatgctccgg aatggaagaa atacagcgaa
     2161 gattttcttg ataagatggc ctggatgcag gatgtgacca cggagaaact gggcccttcg
     2221 ttgtggcata tgcatccaat aatgttttta gcctccctaa ttcaacctaa tgaagatatt
     2281 agaattcttc gtttacgtgc atttctacga atgatccgca tcggtgaagg gacaattcag
     2341 gaagacggat acagaacgat gtttactggt gtaaaattta cagattttag taaacacccc
     2401 aatatcaggc atgaagctaa tggtgttgtt tcgactgctg ctggagctta tcaattttta
     2461 tacggaacat ggagaaattt gcaacgtaga tattcatttc cagatttcag tccgagcaat
     2521 caagatctcg gttgtatagc gctcattgca ggtcgaaaag cattagatgt agtaatgcag
     2581 ggtaagatta gtgaagccat acatttatgt cgtattgagt gggccagttt acccggttca
     2641 ccgcatgctc aaccaactgc aaataaaaaa atgattatgg aaaaatatga actctactta
     2701 gctgaagaaa aagaaggtaa aacaagttta catgccacca cagaaaaaat agtaaaattc
     2761 attgaaggta attacccaga gtacctatga aaaaattcat tttcctgtta ctaattccag
     2821 tgttttgtaa tgcaaacact cctactgttt tcaagtcttc ttgcagtggt attcaataca
     2881 gtatcgaatc attttgtagt gtaaataatc ttaatgatga ccaggataaa cttaccatac
     2941 caatttgtag caaacaaata tttacccttg gtaaggaaaa tcatgaccta tcagagtcta
     3001 caggttttgt tgaacgtatt aacattaaag gcgaaaaaat aaaaatgatg aatttcgttt
     3061 tctacggggc cacatgtaaa agtgatagaa tcattttatc cggcgcaggt ggttgtaatt
     3121 catgtggtga aatgttcaag atatttaatt tatcaggaaa agaaattaat gagaaatcta
     3181 caatgctaga tttggaacct atgcaggata ttattcccga tttttattaa gttaataatt
     3241 gtcatgttgg gaaagtattt agtaaagagt atttattgtc gagttctatg gtttttacct
     3301 tcgtgaattc ggaagtgtga aattcatgca gaaaaaatca aatgttctgc aggatgtttt
     3361 tagggcagcc tgattcagag taaatatctt gttcaaaaac ggttttatcg gttttgttaa
     3421 ttgttttgtt atttttacct gctacggaaa attgatgtga atgggggtta caacctgtat
     3481 aataaataat taaatctgaa taaatataat gcaataacaa aatatgaagc aagtattaaa
     3541 gtaagagaaa ggctaaaatt aggaataaaa gaaagatata gaagttaaca tgagaaaaac
     3601 tctatctttg atagttgcac ttttacctat gtatagcata gacaacagct tcgatttttt
     3661 acagttcatt caataaaaat ggaacacaat gtgaaacctc ttttaatgat aaagttgttt
     3721 tgaagcatag gtgtgagtat gaatatcctt cctgtttgat atcttattct ctccttgctt
     3781 actcttggac agaagtttga atattccatt ccccatggga aatacttgcg aggttggagc
     3841 aaaaagaatt atctctatgg attccgaaaa taaaattcag gtgcataagt caatagatta
     3901 ctgtattgga gttgttggga ttaacaatga tgatgttaaa gtaaaaatta gcattcaaga
     3961 tggtaatgac tctaaaaatt ctgagtgaac aacaaattag caaagttaaa atagttgcgt
     4021 ttcttatctg gtcggtttga tatttttgtt agtat
//
LOCUS       DAZGQL010000207         4024 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.209, whole genome shotgun sequence.
ACCESSION   DAZGQL010000207 DAZGQL010000000
VERSION     DAZGQL010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4024
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.209"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..899
                     /locus_tag="ACHKIR_005052"
     CDS             <1..899
                     /locus_tag="ACHKIR_005052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011192679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO9992652.1"
                     /translation="KIEQAVEELGYIPNRTAGALASGHSDTVAVLVPSLTDKTSSRFM
                     QSLQQVLNKNEFQLLLGCHEYNQLKEAEILMTLLQGNPAALVIFGSQLADKTHQILEK
                     TNIPTINVVGSPFSPAQITIETAFFEASHKLTEHLLEQGYKNIGFIGAHMDNRLQRQQ
                     LNGWHKAMLSYYQNSDLVITMPGAASLQLGRYALNEILLRQPELDAVICSHEEIALGI
                     MFECQRRLLKIPGNIAVACLDGSDSCDQTHPTLTSIRIDYKKMGTETGKLLIGLLNNN
                     HDESEESRIVQFNYQIELRQST"
     gene            1102..1782
                     /locus_tag="ACHKIR_005053"
                     /pseudo
     CDS             1102..1782
                     /locus_tag="ACHKIR_005053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907654.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1799..2107
                     /locus_tag="ACHKIR_005054"
                     /pseudo
     CDS             1799..2107
                     /locus_tag="ACHKIR_005054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611881.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            complement(2210..3316)
                     /locus_tag="ACHKIR_005055"
                     /pseudo
     CDS             complement(2210..3316)
                     /locus_tag="ACHKIR_005055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063816.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
BASE COUNT         1068 a          951 c          937 g         1068 t
ORIGIN      
        1 aaaagataga acaggccgta gaagagttgg gttatattcc caacagaact gctggcgccc
       61 ttgcatctgg tcacagtgat acagtcgccg tactggttcc ttccctgaca gataaaacca
      121 gctcccgctt tatgcagtcg ttgcagcagg tgcttaataa aaatgaattc cagctactgc
      181 ttgggtgtca tgagtataat cagctcaaag aagctgaaat cctgatgacg cttcttcaag
      241 gtaacccggc ggcgttagtt atttttggtt ctcagttggc tgataaaact caccagatac
      301 tcgagaaaac aaatatacct accattaacg ttgtcggttc ccctttcagt ccggctcaaa
      361 ttaccatcga aacagcattt tttgaagcat cacataaact gaccgaacat ctgctggaac
      421 agggttataa gaatatcgga tttatcggtg cgcatatgga caaccgcctg caacgtcagc
      481 aactgaacgg ctggcataaa gcaatgctga gctattacca gaactctgat cttgttatca
      541 ccatgccggg tgccgcaagt ttgcaattgg ggcgttacgc attaaacgag atattactgc
      601 gacaaccaga gctggatgct gtgatttgca gccacgaaga gattgcgctg ggtatcatgt
      661 ttgagtgcca gcggcgtttg ttaaaaatcc ctggaaatat cgctgtagct tgtcttgacg
      721 gttcagacag ttgcgatcaa acacacccaa ccttgacatc catccgtatt gactacaaaa
      781 aaatggggac agagacgggt aagctgctga ttggtctatt gaataataat catgatgaat
      841 cagaagagtc gcgtatcgtg caattcaact accagataga attacgtcag agtacatgag
      901 ctcaaaaggg gattattgca aagttagcta ttgtggtagg gatagccgtt tccggctatt
      961 ccccgcgcag agccggatgt gtgctgctaa cgcatcctgt caacgacgga tgaaaagtta
     1021 tccacttata tctccaccaa cggcccaata ttgtatcgtt cgtgacacat ccatttctac
     1081 ttcaggtatt tggcgtagta gcgggtgcta aaaatttgcc gcaaaaccgc aatatcagcc
     1141 cgcagaggcg ctctccagat tatccattcc tatattatct ctgcaccgga tgaattactc
     1201 gaacaactca gaaatatgac acatatgcaa cttatcagga ctctgggatc ctggaggcct
     1261 gatgccagtg aatacagtaa tgttaccaac gtttatcgca ttacattgaa atcccttgtt
     1321 cgacgctatt tcgagtttca tgatgaaatc accaacttgg atgtcatgat tgcggcaatt
     1381 gttgatgaac tggcacctga actgattaaa cgtaatgctg ttggatacga aagcgctttg
     1441 cagttgttga tctctgccgg agacaatccc taacggttaa gaaccgaatc aggttttgcg
     1501 gcgttgcgcg gtgtcagcca gtttccccta tcttctggaa aaacgaacca ttaccgactt
     1561 aatcggggtg gggagcgtgt tgcaaatagt gcactttaca tcatcactat aggatgttta
     1621 caaactgacg ataaaacaaa ggaatatgtc tccaggcgag tagccgaagg acatacaaaa
     1681 atggaagcaa tacgctgtct gaagcgctat atctcacgcg aagtttatac attactacga
     1741 aatcaaaaca ggcatgttaa ctgcatcccg ataacgtctt gactcttaga agggcgtcca
     1801 aggcagtcat tattcgagca ggcagttccg gcagttattg tgtcgatacc agatcaggca
     1861 gagtatgagc cggcgcggaa aatgctggga taacagctca atggaacgct tcttcaggag
     1921 tctgaagaac gaatggatgc cggtggtggg ttacgtaagc ttcagcgagg cagctcacgc
     1981 cataacggac gatatcgttg gatattacag cgcactaaga ccgcacgaat ataacagtgg
     2041 gttatcccca aacgaatcgg aaaatcgata ctggaaaaac tctaactcgg tggccagttt
     2101 ttgttgacca cttcagccat cgccagacgc taacatgagt tactcttgtt ctgggctacg
     2161 tcaccttcag ggaactggtt atcctcattc tttatttgtg acattgtggt tattgtgcga
     2221 caaacatggc tatcccgccc catatcccag ataaaaccac ctgctcacgt gcgacagcga
     2281 caattgtaat attgacattc tttcctttgg cctgaagttt tcgagacctc ttgcaaagcc
     2341 tgagttgagc atcccatgct ctgtcaatga cggggcgggg taaattttcc tgattttttg
     2401 tattgccgga ctgatgcgtg tcggatgccg gtaactccac gctgcctcaa ccaatagctt
     2461 tctggcatag ctgtttccgg ctttggtaat gctgccctga tgtctgcttc cacccgatga
     2521 atattctgat ggcgtgatac caaaccatga catcagttgt cgtggatgtt caaaacgggt
     2581 cagatcaccc agttcagaaa ttaatccaac cgcagtaata aactgaatcc ctcgcatggc
     2641 ttgctgcgcc tctactaccg gatacagcct ccattcggta acggcctcct tcgggcggat
     2701 tccagccgtt cacattgtgc ctgccggtct tctattgtac aacggtgttc gtcaaaagct
     2761 aattgtcgcc agggactttc gaatgagtat ttgctgagcc agtgtcggtg tgcagggccc
     2821 caatctgctc gcccgacata atggacccca tgaaccagaa gaaacgattt cagacgttgc
     2881 cttgcatgcc gtaaatcatc gcgggcagat gcccatgccc gggccaaatc ccggaatgcc
     2941 tcatcttcaa cgccggatac gtagacagca gaaagatctc ctgcacgcag tgaacgcaca
     3001 agtctgatgg catcacggtg tcggttttac cccgctcccc cggttttttc gggataagtg
     3061 agggggcgca gaccatgcag tcaaaaccgg atttcaccag ccgacgataa agtccataac
     3121 cacagggtcc cgctttataa acgatactga cctgcgatga ctttgacctg atacgttgat
     3181 aaagattatg aatgtcagta ggtgatgtgc taattttacc catcagttca acaggttctg
     3241 aattgatagc ataggagaca gtaatcgatt ctttgtgaac atctaattcg acgtacaaca
     3301 tgttattgtt gttcatgctg gcctccgtgg tggaaattcc aggggggtag caacagctca
     3361 cccatgcggc tctgacaacg atgttaaccc gcattaaata tcccacgttg gacagcatct
     3421 gtctcacgga aagtcatact gccttacgtg catatttcac cttacgcctt cactgtctgt
     3481 catcggaagt actgatttat attggggctt tgcacttact gtcattcacg ggaaaagatg
     3541 gtggtgaaca gtacgatact gtcttggctg ttgccgaggg aagattcagg agtggtaagg
     3601 gggttagtgg tgtaagatga gtctggagct ctgactggtc agtgacgcga aactcaatct
     3661 gccaggataa actccccctt gcttttgcgc agaagcagtg ggccttggtt cgcttatgtc
     3721 ctgcatctac aagcgaatga ctgaccggag actcccatat ctggtcagtc gcccattttt
     3781 attttccacc acatcggaca agcataccgt aataaacagc accagtctcc tgttcactct
     3841 tgcttgattt actggaatgc tgtttatgga gatatccggt tgaaagattt tacctcttcg
     3901 acaggactat aaattataca gtaaggagtc cctcgtagaa aggggctgat tctcatcaag
     3961 tcggacaggt tcaaagtgtt acctgaatga gctaaatgtt atttaccttc ctttctgttc
     4021 attg
//
LOCUS       DAZGQL010000208         3990 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.210, whole genome shotgun sequence.
ACCESSION   DAZGQL010000208 DAZGQL010000000
VERSION     DAZGQL010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3990
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.210"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            35..1693
                     /gene="dpiB"
                     /locus_tag="ACHKIR_005056"
     CDS             35..1693
                     /gene="dpiB"
                     /locus_tag="ACHKIR_005056"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HFO9992653.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSIISAVKTRDYKRLATIADKLQRDTDFDYVVIGD
                     RHSIRLYHPNPEKIGYPMQFTKPGALEKGESYFITGKGSIGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIHSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHLNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMVIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
     gene            1662..2342
                     /gene="dpiA"
                     /locus_tag="ACHKIR_005057"
                     /gene_synonym="citB"
     CDS             1662..2342
                     /gene="dpiA"
                     /locus_tag="ACHKIR_005057"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HFO9992654.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISRTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(2383..3768)
                     /gene="dcuC"
                     /locus_tag="ACHKIR_005058"
     CDS             complement(2383..3768)
                     /gene="dcuC"
                     /locus_tag="ACHKIR_005058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFO9992655.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFIRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
BASE COUNT         1089 a          943 c         1032 g          926 t
ORIGIN      
        1 tcaaagaggc tcgggacaac ccgcaaggaa aacaatgttg cagcttaacg agaataaaca
       61 gtttgcattt ttccaaagac tggcatttcc gctgcgtatc tttttgctga ttctggtgtt
      121 ctcaatattt gtcattgcag ccctggcgca atattttacg gccagttttg aggactattt
      181 aacgcttcat gtgcgcgata tggcaatgaa tcaggcaaaa attattgctt ctaatgacag
      241 tatcatcagc gcagtgaaaa cgcgtgacta caagaggctg gcgaccatcg ctgataaatt
      301 acaaagagat actgatttcg attatgtggt gattggcgat cggcactcga ttcgtcttta
      361 ccatcctaat ccggagaaaa ttggttatcc tatgcagttc accaaaccgg gcgcgctgga
      421 gaaaggggag agctacttca tcaccgggaa agggtcaatt ggcatggcga tgcgtgccaa
      481 aacgccaatc tttgatgacg atggaaaagt catcggcgtg gtgtcgattg gctacctggt
      541 gagtaaaatc gatagctggc gggctgagtt tttattaccg atggctggcg tgtttgtcgt
      601 gctgttaggg attctgatgt tgctgtcgtg gttcctggcc gcgcatatcc gtcggcagat
      661 gatgggcatg gagccaaagc aaatcgcacg cgtggtccgt cagcaagagg cgctgtttag
      721 ttcggtttat gaagggctca ttgcggtgga tccgcacggt tacattaccg ccatcaatcg
      781 taacgcaaga aagatgctgg ggctgagctc ccccggacgg caatggttgg gtaaacccat
      841 tgctgaagtg gtcaggcccg ccgatttctt taccgaacag attgatgaaa aacgtcagga
      901 tgtggtggcg aactttaacg gtctgagcgt tattgccaac cgggaagcta ttcattctgg
      961 tgatgatttg ctgggggcca ttatcagctt tcgtagtaaa gacgaaatat ccaccctcaa
     1021 tgcacaactg acgcaaatta aacaatacgt cgagagcctg cgcacattgc gacacgagca
     1081 tctcaattgg atgtcgacgc tcaatggtct gttgcagatg aaagagtatg atcgcgtgtt
     1141 ggcgatggtg cagggggagt ctcaggccca gcaacagctt attgatagcc tgcgcgaggc
     1201 gtttgccgat cgccaggtgg cggggctgct ttttggtaaa gtgcagcgcg cccgcgaact
     1261 ggggctaaaa atggtcatcg tccccggaag tcagctttcg caactgccgc caggactgga
     1321 cagcaccgag tttgcagcca ttgttggcaa tttacttgat aacgccttcg aagccagcct
     1381 gcgtagcgat gaaggaaaca agatcgttga attattcctc agcgatgaag gcgatgatgt
     1441 ggtgattgaa gtcgccgatc agggctgcgg cgttccagag tctctacgag acaaaatatt
     1501 tgagcagggt gtcagtacgc gtgctgacga gcccggcgaa catggcattg ggttgtactt
     1561 gattgccagc tacgtaacgc gctgcggtgg tgttatcact ctcgaagata atgatccctg
     1621 cggtacctta ttttcaatct atattccgaa agtgaaacct aatgacagct ccattaaccc
     1681 tattgatcgt tgaggacgaa acgccgctgg cagagatgca tgcggaatat attcgtcaca
     1741 ttcccggatt cagtcagata ttactggcgg gaaacctggc gcaggcccga atgatgatcg
     1801 agcgttttaa gccggggcta atcttgctcg ataactatct tcctgacggt agagggatta
     1861 atttactgca tgaactggtg caggcgcatt atcccggcga cgtggtgttt accactgcag
     1921 ccagtgatat ggaaacggtg tctgaagccg tacgttgtgg tgtatttgat tatctcatta
     1981 agccaattgc ctatgaacgg ctggggcaaa cgctaacccg tttccgccag cgtaaacata
     2041 tgctggaaag tattgatagc gccagccaga agcaaatcga tgagatgttt aatgcctatg
     2101 ctcgcggtga acccaaagac gaactgccga ccggcattga ccccttgacg ctaaacgcgg
     2161 tgcgaaaact gtttaaagag cctggtgtgc aacatacggc agaaacggtg gcgcaggcac
     2221 tgactatcag ccgcaccact gccagacgtt atcttgaata ttgcgccagc cgccatctga
     2281 ttattgctga aattgttcac ggcaaagttg gcagaccaca acgcatatac cacagtgggt
     2341 gacatgataa agccggagga aacttgcctc cggcatgact atttacttgc ctgtgaccgc
     2401 ggctgccgtt cctggcacca tcagctctgt agcaacgata acaatcacca gcccaacaag
     2461 caccggcacc gaggtgcgtt ttacgacttc aaatggtgag attttcgcca tcccggcaac
     2521 cgcaacgact acgccagaaa cgggtgacag ggtacggccg aggtttgacg cctgcagcat
     2581 tgggatagtc aaatacgccg ggttaatgcc ggaagagtgc gccagtttcg ggatcatctc
     2641 aacaaacgca taaaacggcg cattgcctga accggtcgtg actgccgcca gcattgtcag
     2701 gatcaccaat accagcatca ggatgatgct cgccgaacca aacgaggtag cgatagaaat
     2761 cagactttga ataaagccga tggtgctaag cccctgagcg aataccccag cggcaaccag
     2821 cagcatcacc acgtttgcaa atgcatctgc catcccgcga taagccactt ccagaccaga
     2881 gaaaactttc tgggtattaa agctgcggat gaactccaga atggaggcaa tcagcataca
     2941 aatcaccaga atagtgatga tgtgtaattg cggaccccat ttgccgtcaa aaatcagcac
     3001 tccgatgatc ggcgtgaacg gcaaaatggc ataaaacgca ggggcagtgg tggtgatttc
     3061 actgacatct aacatttcat gagagatgtg ttctttttta tccagataac gttgccagaa
     3121 gaagtgggcg attgccatgc cgataattgc agcaattgag ataggcagcg ttgttttgaa
     3181 ggcgaagtca atcagcgaca tttcggaagc ctgcgccgcc agcaccacat cccctgaagt
     3241 cggtgcgaga ataatcgccg ccggggaggc acaaatagca gcagctgcac cacggctgat
     3301 accaacgttt accatcaccg gaaacagggt tgccatcagc aaaacaccca gaccggtcgc
     3361 ggaagagacg gctagagaca tcagacaggc gacaaaatag gcggcaatca tcagcaggta
     3421 aggggagtta atatactgca atggttttga cgccaacttg accaccatat cattcgcgcc
     3481 gatatgggtc atgtaagcgg caaagccaca cagcatcata atcatcatgc cgaggtcgcc
     3541 gccgcggctc attagcaata ttttaacgta ttcaacgata tccgtggcgc tgtagcctgt
     3601 tgaagcctgg ctggacggta acactttgtg ccccataatg gcactgataa tcagcaataa
     3661 caggccaccg acaaataaca cgccagtggc ggaataccct ttaatgatgt agcgagctac
     3721 acccacaata accacaaccc caataaggag ttcaatgaat gtcagcataa tttttcctgt
     3781 ctccaggccc caaagtaaat aataaaaaat tcctaaagct taaggaaaaa atatgcccaa
     3841 taaattggcg atgaatgctg attaaaatca agaaaaactg ccattaagac attgaagttg
     3901 ctgtttttat acatagataa caaaacgcat atttagcggt tggcaattat cacataataa
     3961 tgtgtgtaaa ttgatggtgg attgatgcaa
//
LOCUS       DAZGQL010000209         3981 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.211, whole genome shotgun sequence.
ACCESSION   DAZGQL010000209 DAZGQL010000000
VERSION     DAZGQL010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3981
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.211"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(99..1778)
                     /gene="bcsG"
                     /locus_tag="ACHKIR_005059"
     CDS             complement(99..1778)
                     /gene="bcsG"
                     /locus_tag="ACHKIR_005059"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HFO9992656.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLFVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDITEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFSEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(1775..1966)
                     /gene="bcsF"
                     /locus_tag="ACHKIR_005060"
     CDS             complement(1775..1966)
                     /gene="bcsF"
                     /locus_tag="ACHKIR_005060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0052324 - plant-type cell wall cellulose
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HFO9992657.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(1963..3534)
                     /gene="bcsE"
                     /locus_tag="ACHKIR_005061"
     CDS             complement(1963..3534)
                     /gene="bcsE"
                     /locus_tag="ACHKIR_005061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis c-di-GMP-binding protein
                     BcsE"
                     /protein_id="HFO9992658.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPGKITLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINSGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGCWTLVQHQEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            3807..>3981
                     /gene="bcsR"
                     /locus_tag="ACHKIR_005062"
     CDS             3807..>3981
                     /gene="bcsR"
                     /locus_tag="ACHKIR_005062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsR"
                     /protein_id="HFO9992659.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDILALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEF"
BASE COUNT         1040 a          974 c         1004 g          963 t
ORIGIN      
        1 aagcggaagt aacgtgtcat gcgggcgtca ggctgccgta atggcaattt gcgcccggat
       61 caggccgcag gggtgaaact ctgcggcctt tttcgttctt attgcgggta aggcacccag
      121 tcgccgccgt tcaggcgaac gtacggttta tcctggtatt gaataactac tgcatttgag
      181 ttctcggaga ccggtgctgt ttgtggcaac ccactggtga gttttttcca gtcaacattg
      241 tcttcggaga aaatcttgcc atcaagaacg cgaaccacca gatcggagat agccaggaag
      301 ctgctcggtt ggtcgatgac aatcggtgcc ccctgatgtg gtgccttcat gccgaagaat
      361 ttcaccccaa cggggacgtc tgtgatagac gggctaggga tatcacgtag gccagatacc
      421 tgcattctgt cgcccttcag cgcgccgccg tgttccggca ccacgaccac catcacttta
      481 cgacccgatt tctccagttc agtaaagaag gcgtccagtt catcaaagaa tttctgcgcc
      541 cgcgctttgt aatccgctgt tttgctgacg cccggataat ggttgccgtc atgcagtgga
      601 agcgtgttgt agaacgtggc actacggctg tttttatctt tttcggtaat gtccagccag
      661 cggttaagca cggcggtatc gtcataaacc ggcgaaccat caaagcccag caaaataacc
      721 ggcagatttg tttgatccat caattcggtc tgcatgccgc cattttcgcg aacttctttc
      781 aaaaaaccgc cgaactggcc gttatgtccc atcatcaggt gctgggtaaa gcccagtttc
      841 gacaggttat caaacagata gcagtcgtta tttgccggtt gatacagatt agtgtgcgaa
      901 gtctgcccgc agctggcgcg cagtaaacgg atcgccgccg ggccactgta ggaggtggcg
      961 gagttaaagt tcttgaactc aatatcgaaa tgcgaccaca gcggatgcga catcaacccg
     1021 gcggcttcaa tatccgacca ggaaagcgaa cagatattaa tcaccagtag ttcaaatggc
     1081 tgagcatcag cgggcagcga agacgggaag gtcgatttac gtttcgcctc cgcgttatag
     1141 aaattattta gccaggcgtt aaggttcgcc gttgttggcg gtgcagtttg tgcgggcata
     1201 tcacccacta ccggtgcgcc acccgtcgcc gcaacggttg ccgctgcgtt accacccgtc
     1261 gttgttacag tggtcgtcgg ttgaccggct ggccacaagg agaaacttgg tcccgccagg
     1321 gtaagtacgt tcagccatag cagaatggca accacaaaaa cggtaatgcg aatccattgt
     1381 gacaggaata accaggccac gaataaaaca aaaatggccc caatcatctg ccagttaata
     1441 aagcgtgtga caaggtcgat taaataatcg gtactgaacc ccgccacctg cgaaccctgg
     1501 ctcattatgc tttccgggcc aggcaaccag gtgtcatgcc agaacaaagc aaagccgatc
     1561 ggcagggcaa tccagtggcg caagcgatgc aggctgtagc gcggaatggg catcagcaga
     1621 aacgcggcaa agaccaaatt gaggagcgga tggaagttaa gatatcccgc ccacaacagg
     1681 ccgaacttaa ccagaaaata gaagttccag ccggaaaggc cgcgccagta ttgccagagg
     1741 gaagaaggca tggcggtatt ttgcgtaaat tgagtcattt tttggttgcc ctggcttttt
     1801 ccgtgcggcg taacgtcccg gccggtttaa cataacgagg tttagcaaag aacaaacgta
     1861 aggtgtcgcg aatgcgtcgc aaagagtgcc gcgccagata gcccagcggg aaaaatatca
     1921 gtgcgcaaac gacaataatt tcaatgatat cgctgatggt catcatgatg agcgctccac
     1981 agcatcatct aacagtcgca ttggttctgg tattcgtcgc cagtggcgac catcgtgctc
     2041 ggcattgatg accggtttag aactttgcgt taaaggcagc ggcatgcccc attgttctgg
     2101 ggcaagcagg cgcatctgca ccagctcggc actgatttga tcatcttcaa accagaccat
     2161 acggtttgag aaaatgtcgc cagtaggcaa tgggaaaata tgattcaacg cggtatccag
     2221 atcgttaatc cgacagaatg agagaaacag caccagccga ttaccgccaa tggtcatgat
     2281 atcgccggtg cgattagggc gacatagcgt cagggcttgc tcaacgcgga tacccggtac
     2341 cggacgtagg gcaaccagaa cgcctttacc gtgggcaggt aatagagggt tattcatcat
     2401 gttgttgacg gcattacaga acacatccca cttctggaaa ccacgcagtt tgagcggctg
     2461 ggtcattgac agcaaggtag tgatatcttc cggcacatag cgactaaact tctgcccttg
     2521 cacgctttcg atcatcgtca gacagcggga gagtggcgca ttccacggga taaccatatt
     2581 tgcaccgcag gccaataata aacgttcatc ggtggcgcgc aggctagcgg tattttcccg
     2641 tacgaggatt ttcatcgcac taccgcgctg gcgacgcaga gtatgaatgc tgcgggccag
     2701 tggctcgatt tgcgcatttt gctgtaaaga aaagaccacc gtcgccgcct gagcggtacg
     2761 ggcttcattg aacaggactt cgttattgtt gaacagttgc cagtgttccg atagcggcgg
     2821 cgcaccttcc agtacagcaa cattactcag aatgcgtttt tcgtcgctgc gtggttggat
     2881 ttccgcctcc tggtgttgaa ctaatgtcca gcaaccattt tgttgctgaa cgctaagctg
     2941 ctgacgggcg ctgacccctt tttcgttgca ccagaaagca atatccagca aatgttggtc
     3001 gccctgaaaa cgcaaactgg caagaccaaa aagtgaacgg tattcctcaa gtaacaatga
     3061 aaattgttta tcgttattat ttccggaatt aattaccaaa agcgaacaat ggtttagccg
     3121 gctccattta ttcattttat ccaaccatga gcgaagccgc tcggcaggaa tgttttgcca
     3181 tgcgttattt gcgcaaacaa gaataaaaag gtaattatgg ggatcaatag aacactgcaa
     3241 atcacgggcc aaatagtata gacctttttc atgatttagc attgaaaata atgttatttt
     3301 tcccggccct tgagaatcat ctaattgaaa gattttcgct ggatcgctgt ccatgctaat
     3361 gaccgcgacg tgtgcggttt cagcctggga tgcaattgtt tgattcgcca gactgatagc
     3421 atcttcatgg cgatcgacgt taaaccacca gacgccgcct gctggcatat gtcgcagctc
     3481 atcccataat gatgagatac cgatagagaa tacagggtcc acaatgtccc tcatgtcaac
     3541 attttgtctg attatcagtt tactagcgaa agctaagaaa taaacctacc attgaaatta
     3601 aaacttatca gatttagcat gtgaatcaat ttagcaggtc acaagacatg tgattttagt
     3661 cttttcgttt ttaacttttt ctgttgcgta acggaaagtc aaaaagtgag cacattcccg
     3721 tctcgccgta aacaataaac cgacgaaatg ctcacagtta gaattgtctc atgaacggta
     3781 cggttatttc atagggatca agaaaaatga ataacaatga accagatact ctgcctgatc
     3841 ccgcgatagg ctatatcttc cagaatgata ttttggcgtt aaagcaggca ttttcactgc
     3901 ctgatattga ttatgccgat atttcccaac gcgaacagtt ggccgcggca ttaaaacgct
     3961 ggccgttgct ggcagagttt g
//
LOCUS       DAZGQL010000210         3955 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.212, whole genome shotgun sequence.
ACCESSION   DAZGQL010000210 DAZGQL010000000
VERSION     DAZGQL010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3955
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.212"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            37..1119
                     /locus_tag="ACHKIR_005063"
     CDS             37..1119
                     /locus_tag="ACHKIR_005063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpD"
                     /protein_id="HFO9992660.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYARLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNNQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLVEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGMVYQF"
     gene            complement(1308..1691)
                     /gene="quuD"
                     /locus_tag="ACHKIR_005064"
     CDS             complement(1308..1691)
                     /gene="quuD"
                     /locus_tag="ACHKIR_005064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein QuuD"
                     /protein_id="HFO9992661.1"
                     /translation="MRDIQMVLERWGAWAANNHEDVTWSSIAAGFKGLITSKVKSRPQ
                     CCDDDAMIICGCMARLKKNNSDLHDLLVDYYVVGMTFMSLAGKHCCSDGYIGKRLQKA
                     EGIIEGMLMALDIRLEMDIVVNNSN"
     gene            complement(1777..1917)
                     /gene="ylcG"
                     /locus_tag="ACHKIR_005065"
     CDS             complement(1777..1917)
                     /gene="ylcG"
                     /locus_tag="ACHKIR_005065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HFO9992662.1"
                     /translation="MMFEFYMAELLRHRWGHLRLYRFPGSVLTDYRILKNYAKTLTGA
                     GV"
     gene            complement(1914..2276)
                     /gene="rusA"
                     /locus_tag="ACHKIR_005066"
     CDS             complement(1914..2276)
                     /gene="rusA"
                     /locus_tag="ACHKIR_005066"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RusA"
                     /protein_id="HFO9992663.1"
                     /translation="MNTYSITLPWPPSNNRYYRHNRGRTHVSAEGQAYRDNVARIIKN
                     AMLDIGLAMPVKIRIECHMPDRRRRDLDNLQKAAFDALTKAGFWLDDAQVVDYRVVKM
                     PVTKGGRLELTITEMGNE"
     gene            complement(2273..2563)
                     /gene="ybcO"
                     /locus_tag="ACHKIR_005067"
     CDS             complement(2273..2563)
                     /gene="ybcO"
                     /locus_tag="ACHKIR_005067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFO9992664.1"
                     /translation="MADLRKAARGRECQVRIPGVCNGNSETSVLAHIRLAGLCGTGIK
                     PPDLIATIACSACHDEIDRRTHFVDAAYAKECALEGMARTQVIWLKEGVIKA"
     gene            complement(2556..2726)
                     /gene="ninE"
                     /locus_tag="ACHKIR_005068"
     CDS             complement(2556..2726)
                     /gene="ninE"
                     /locus_tag="ACHKIR_005068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFO9992665.1"
                     /translation="MATPLIRVMNGHIYRVSNRRKRKPELKPSEIPTLLGYTASLVDK
                     KWLRLAARRNHG"
     gene            complement(2726..3181)
                     /gene="ybcN"
                     /locus_tag="ACHKIR_005069"
     CDS             complement(2726..3181)
                     /gene="ybcN"
                     /locus_tag="ACHKIR_005069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA base-flipping protein YbcN"
                     /protein_id="HFO9992666.1"
                     /translation="MNLSQDGIKLHRGNFTAIGRQIQPYLEEGKCFRMVLKPWREKRS
                     LSQNALSHMWYSEISEYLISRGKTFATPAWVKDALKHTYLGYETKDLVDVVTGDITTI
                     QSLRHTSDLDTGEMYVFLCKVEAWAVNIGCHLTIPQSCEFQLLRDKQEA"
     gene            complement(3178..3279)
                     /locus_tag="ACHKIR_005070"
     CDS             complement(3178..3279)
                     /locus_tag="ACHKIR_005070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992667.1"
                     /translation="MRTYNPNSLLPSQMQKCTCNSLHLAFDLCGGEA"
     gene            complement(3396..>3955)
                     /locus_tag="ACHKIR_005071"
     CDS             complement(3396..>3955)
                     /locus_tag="ACHKIR_005071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO9992668.1"
                     /translation="LYLPIDLIESFQKLYTVTDQIRNKTSFFLPQNPELIYCWEQLKT
                     SVSRGFSTKIQEHLAMGVLLSLGVNHVNHLLLSYSKQSLISRCYNLLLSEPGTKWTAN
                     KVARYLYISVSTLHRRLASEGVSFQSILDDVRLNNALSAIQTTVKPISEIARENGYKC
                     PSRFTERFHNRFNITPREIRKASRE"
BASE COUNT         1076 a          913 c          857 g         1109 t
ORIGIN      
        1 cattttttaa taatagttca aaaaaaaggg ctcacgatga aaaaattaac agtggcaatt
       61 tctgctgtag ctgcatcagt actgatggcg atgtctgctc aggcagctga aatttataat
      121 aaagacagta acaagctgga tctatatggg aaagttaatg ccaagcacta cttctcctct
      181 aatgatgcag atgatggtga tactacttat gcccgtcttg gcttcaaagg tgaaacccaa
      241 atcaacgatc aactgactgg tttcggtcag tgggaatatg aattcaaagg caaccgcgct
      301 gaatctcaag gctcctccaa agacaaaacc cgtcttgcat ttgcaggcct gaaattcggg
      361 gactacggct caatcgatta cggccgtaac tacggtgtag catatgacat cggtgcgtgg
      421 actgacgttc tgccagaatt cggtggcgat acctggaccc aaacagatgt gttcatgact
      481 ggtcgcacca ctggtgttgc aacttatcgt aacaacgact tctttggtct ggttgatggt
      541 ctgaactttg ctgctcagta tcagggtaaa aatgaccgca ctgacgtaac tgaagctaat
      601 ggtgatggtt tcggtttctc cactacttat gagtatgaag gattcggtgt aggtgcaacc
      661 tatgctaaat ctgaccgcac taataatcag gttatctacg gtaacaacag cctgaatgca
      721 tctggtcaaa atgctgaagt atgggcagct ggtctgaaat atgatgcgaa caacatctat
      781 ctggctacca cctattctga aacccagaac atgactgttt ttggtaataa ccatattgcc
      841 aacaaagcac aaaacttcga agtagttgca caatatcagt tcgacttcgg tctgcgtccg
      901 tccgttgctt acctgcaatc taaaggaaaa gacttgggtg cgtggggtga tcaggacctg
      961 gttgaatata ttgatgtagg tgcaacctat tacttcaaca aaaatatgtc cacttttgtt
     1021 gattacaaaa tcaacctgat tgataagagc gatttcacga aagcatctgg cgttgctacc
     1081 gatgatatcg ttgctgtagg tatggtttac cagttctaat ttgattacta aaagatatgt
     1141 tgcgggaggc tttgcctccc caacatataa gtggctccct caagccactt cctttaggag
     1201 cacaaccttg cttctaacta tataaacctt ctgttatata ttacccttta tttttggggg
     1261 cgttgcaacg ccccattttt aataattttt agtaaacaat tggcatatta attagagtta
     1321 ttaacaacga tatccatctc taaccggata tctaatgcca ttaacatccc ttcaattatg
     1381 ccctcagcct tctgtaacct tttcccgata taaccatcag agcagcaatg cttacctgcc
     1441 agtgacatga atgtcatacc gactacataa taatctacta ataaatcgtg caaatcgctg
     1501 ttgttctttt tcagacgggc catgcacccg caaataatca tcgcgtcatc gtcacaacat
     1561 tgcgggcgag attttacttt tgaagtaatt aatcccttaa aaccggcggc aatggacgac
     1621 caggtcacat cttcatgatt attagccgcc cacgctcccc aacgctcaag aaccatctga
     1681 atatcacgca tcaacttact ccacaaaaat cagaccagaa cgccaattac aagcaaaaat
     1741 caacaaaaca gtattagttg attgttatct ctgacttcat actcctgctc ctgtcagggt
     1801 tttggcgtaa ttcttcagta ttcggtaatc ggtcaaaaca gaaccgggga aacgatataa
     1861 gcgcagatgc ccccagcggt ggcgaagaag ttctgccata taaaactcaa acatcattca
     1921 ttccccattt cggtgatggt cagttccagc ctgccacctt tggtaacagg catcttcaca
     1981 acgcggtaat caacgacctg agcatcatcc agccagaaac ctgctttggt gagtgcgtca
     2041 aaagcggctt tttgcagatt atccaggtca cggcgacggc gatccggcat gtggcactca
     2101 atgcggattt tcacaggcat agccaggccg atatccagca ttgcgttttt aatgattcgg
     2161 gcgacgttat cgcggtatgc ctgcccctct gcactgacgt gcgtgcgccc gcgattatgg
     2221 cggtaatagc gattattgct cggaggccag ggtaatgtga tactgtaggt attcacgcct
     2281 taataacccc ctctttcagc cagataacct gtgttctcgc cataccttcc agcgcgcatt
     2341 cttttgcata tgcagcatcg acaaaatgtg tgcggcggtc gatttcgtcg tggcaggcag
     2401 aacatgcaat ggtggcaatc aggtctggcg gtttgatacc ggtaccgcac aatccagcca
     2461 gccggatatg tgccagtaca gacgtttcag aattgccatt acatacgcca gggattctta
     2521 cctggcattc ccgaccacgc gctgcttttc tcaaatcagc catgattcct ccttgctgcc
     2581 agtcgcaacc attttttatc aaccaggcta gcggtatatc cgagcagtgt tggtatttcg
     2641 gatggcttca gctcaggctt acgcttacga cgatttgata ctctgtagat gtgtccgttc
     2701 atgacacgaa taagcggtgt agccattacg cctcctgctt gtcgcggagc agctggaact
     2761 cgcagctctg cggaatagtc aggtggcagc caatattcac cgcccaggct tcaaccttac
     2821 acaggaagac atacatctct ccggtatcaa gatcggaggt atggcgtaac gactggatag
     2881 tggtgatatc accggttacg acatcaacca ggtctttggt ttcataaccg agatatgtgt
     2941 gtttgagagc atcttttacc caagctggag tggcgaacgt tttacccctg ctgatgaggt
     3001 attcactgat ttcgctgtac cacatgtggc tgagtgcatt ctgggaaaga ctgcgtttct
     3061 cacgccacgg tttaagcacc atgcgaaagc atttgccctc ctccagataa ggctggatct
     3121 gccgaccgat agcggtgaag ttgccgcgat gtaatttgat gccgtcttgt gagaggttca
     3181 cgcttcacct ccgcagaggt caaacgctag atgcaaagaa ttgcaggtgc atttctgcat
     3241 ctgtgaaggg agaagagagt ttggattgta tgtgcgcata aacgtccccg tttagcgcag
     3301 aagtcaccgg agttgttcag gctccggtga cataattatg ccgtgttgat ttcccaaaat
     3361 caaaatcgat agaattgctc cttcttaaaa cacttttact ctctggaagc ttttcttatc
     3421 tctcttggtg ttatattaaa acgattatga aatctttcag taaaacgaga aggacactta
     3481 taaccatttt ctctggcaat ctcgcttata ggttttaccg tcgtttgtat agcagacaac
     3541 gcattattta acctcacatc gtccagtata ctttggaaac ttaccccctc gcttgctaga
     3601 cggcgatgta atgtagaaac agaaatgtag agatatcgag caaccttgtt tgctgtccat
     3661 tttgtgccgg gttcggatag cagcaggtta taacaacgac ttatcaatga ttgtttacta
     3721 tatgataaaa gtaaatgatt aacatgattc actcctaacg aaagtagaac gcccattgct
     3781 aagtgctcct gaattttagt tgagaagcct cgggaaacag atgtttttag ttgctcccaa
     3841 caatatatta actcaggatt ctgaggtaaa aagaaacttg ttttgttacg tatttgatca
     3901 gttaccgtat aaagtttttg gaaactctca attaaatcaa tgggtaagta aagca
//
LOCUS       DAZGQL010000211         3884 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.213, whole genome shotgun sequence.
ACCESSION   DAZGQL010000211 DAZGQL010000000
VERSION     DAZGQL010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3884
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.213"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..546
                     /locus_tag="ACHKIR_005072"
     CDS             <1..546
                     /locus_tag="ACHKIR_005072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2345 domain-containing protein"
                     /protein_id="HFO9992669.1"
                     /translation="IRFVTDKIRELQSAVLLGSAPQGVALTSGEHLQLCSTRNTMINA
                     GQHLDIGAMKNLSVTVEKALGLFVHKDGAKLVANQGDIEIQAQHNTMALFSEKQLTVT
                     SSEDEIIISTPETLTLNGGGSYLRLSKNGIEHGSSGEFIVKTSDYLVPGSGVDLPNEA
                     PGFSLTDITQESKISSKSFND"
     gene            562..2886
                     /locus_tag="ACHKIR_005073"
     CDS             562..2886
                     /locus_tag="ACHKIR_005073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992670.1"
                     /translation="MSVLPKIVWPIPSSRRGSEFKNQEEILSHLGGESTGLYMIGRSG
                     MWHGGIHITEATTPWCALSGKSPLEAIDFPVPFKGEQAIRCMADGEVIAYRICRDYLT
                     IAWESGPLNFSGSFVLVKHYIQPGEKETSGLHFYTLYMHLAPYSAYESEAENQWVVQD
                     TLKAYSEMDWLTAKLTSDETSPQIAGYMPEGARVEWNPADSNLNATGSNKRKYGLVTL
                     KGLPEKNSSTLTPGQRYWVVVDNNNVKPAPGAGPGWWRHLLPPAKEAMVFDKTVSLSS
                     PFAIKVGDPIGHMGYYQAPKDGGYEARYQVHIECTSMDDNLEKFLTNPEQVGKNNPLW
                     LKYAPGLALYKKDAFTGTFTKDARVTTRTGILPLKQVQTEVDKSTKQEYWQLRPENAY
                     VPKGQVEPQLLSQYDLAKLGFRTEIAEPTSFDYLDGKKQPTGFFRSLINSLYEAANDD
                     TRTSHALVKHNYQRLLDKIDSGSDRYSPMEYWRALHNPDYRDVLQKTIVKHPSDWYFK
                     KDDAIWQPFLNALKKDAPEWKKYSEDFLDKMAWMQDVTTEKLGPLLWHMHPIMFLGCM
                     KKSSGIWPLGKTSERYESGGRGPGVISSGKGDHGGVSYGMYQLSSKMGTVNKYIIMSK
                     YKDLFEGKKPATPEFNEIWKKIALIHKEEFAQDQHLFIKKTHYQVQLDYLASKGLNFS
                     HSLAAINDMIWSTSVQYGPTTGLISRALKGKNLETLTDADVITIVQDYKINHVEDLFP
                     SSPTWWDDLKTRALTEKKDLLELNELNLELKNED"
     gene            2876..3232
                     /locus_tag="ACHKIR_005074"
     CDS             2876..3232
                     /locus_tag="ACHKIR_005074"
                     /inference="COORDINATES: protein motif:HMM:NF045244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992671.1"
                     /translation="MKIKLFALLIFLPIISFAKEPHVCNSSMQSHPDIIDCIQISTRK
                     IEDSIKEIIAKSGNEYSIDMAFYNSQRKSISERCSLYYSMEGQRIDLIENQCEYDGVE
                     QLNKFIKTYMKTVDNY"
BASE COUNT         1212 a          735 c          907 g         1030 t
ORIGIN      
        1 attaggtttg tgaccgacaa aatcagggaa cttcagtctg cggtcctgct gggtagtgcg
       61 ccgcaggggg tggccctgac ctcaggtgaa caccttcagc tctgcagtac ccgtaacacc
      121 atgatcaatg caggccagca tcttgatatc ggtgcgatga aaaatctgtc ggtaacggtg
      181 gaaaaagccc ttggtctgtt tgtgcataaa gacggggcga agctggtagc caaccaggga
      241 gatattgaga ttcaggctca gcacaacacg atggcgctgt tctctgaaaa acagctgacg
      301 gtgaccagta gtgaagacga aattattatc agcaccccgg aaactctgac gctgaacggt
      361 ggtgggtctt acctgagact gagtaagaac gggatagagc acggctcatc gggtgagttt
      421 attgtgaaga cgtctgacta tctggtgcct ggttctggcg tagatctccc caacgaagca
      481 cctggtttca gcctgacgga tatcacacag gaaagcaaaa tcagcagcaa gtcttttaac
      541 gactgacagg gagtatgaat cgtgtctgta ttacccaaaa tcgtctggcc gatcccctcc
      601 agccgtcggg gaagtgagtt caaaaatcag gaagagatcc tctctcattt ggggggagaa
      661 tctaccgggc tgtacatgat aggtcgtagt ggcatgtggc atggtgggat ccatatcacc
      721 gaagccacga caccatggtg tgcgcttagc ggtaagtctc cacttgaagc gatagatttt
      781 ccggttcctt ttaaaggtga gcaggcaatc cgatgcatgg ctgacggaga agtgattgct
      841 taccgcatct gcagggatta tctgacgatt gcatgggaat ccggtccgct gaatttttcc
      901 ggctcatttg ttctggtgaa acactatatc cagccgggtg agaaagaaac cagtggactg
      961 catttctata ctctgtatat gcatcttgcg ccgtattcag cttatgaatc tgaagcagaa
     1021 aatcagtggg tcgttcagga cactctaaaa gcatattctg aaatggactg gctgacggcg
     1081 aaactgactt cagatgaaac ttctccacaa attgcagggt acatgcctga aggggccaga
     1141 gtggagtgga acccggcaga cagcaatctt aacgctacag gaagtaacaa gagaaaatac
     1201 ggtcttgtga cgctaaaggg tttaccagaa aaaaacagca gtactctcac tcccggtcag
     1261 cgctactggg ttgttgtgga taacaacaat gttaagccag cgccgggggc cgggcccggc
     1321 tggtggcgac acttgctgcc tcccgcaaag gaagccatgg tattcgataa aacagtaagc
     1381 ctcagttctc catttgccat taaggtcggt gacccgattg ggcacatggg ctactatcag
     1441 gccccaaagg atggggggta tgaggcacga tatcaggtac atatcgaatg cacgagtatg
     1501 gatgataatc tggagaaatt cctgaccaat cctgagcagg tgggaaaaaa taatccactc
     1561 tggctgaagt acgccccggg gcttgcgttg tacaagaaag atgcttttac aggaacattc
     1621 acgaaggacg cgagagtgac gaccaggacg ggaattctgc cactgaaaca ggtgcaaaca
     1681 gaagtcgata agtcgaccaa acaggaatac tggcaattgc ggccagaaaa cgcgtacgtg
     1741 ccaaaaggtc aggtggaacc tcaacttttg tcacagtatg atctggcaaa gctggggttc
     1801 agaactgaaa tcgcagagcc taccagcttt gattacctgg atggaaaaaa acagcccact
     1861 ggattcttcc gcagtcttat caactcactc tatgaggctg cgaacgatga tacgcgaacc
     1921 agtcatgcgc tggtgaaaca taattatcag cgcctgctgg ataaaattga tagtggaagc
     1981 gaccgttact ctccaatgga atactggaga gcacttcata accccgacta tcgtgatgtt
     2041 cttcagaaaa caatcgttaa acatcccagc gactggtact tcaaaaaaga cgatgcaatc
     2101 tggcaaccat ttctgaatgc gctgaaaaaa gatgctccgg aatggaagaa atacagcgaa
     2161 gattttcttg ataagatggc ctggatgcag gatgtgacca cggagaaact gggccctttg
     2221 ttgtggcata tgcatccgat tatgttctta ggctgtatga aaaaatcgtc aggaatatgg
     2281 ccacttggga aaacttcaga gcgttatgag tctggaggaa gagggccagg tgtaatttca
     2341 tctggaaaag gagatcatgg cggtgtgtca tatggaatgt atcaattatc ttctaagatg
     2401 ggaacggtta ataaatatat tataatgtca aaatacaaag atttatttga gggtaaaaaa
     2461 cctgctacac ctgaattcaa tgaaatatgg aaaaaaattg ctctcattca caaagaagaa
     2521 ttcgcacaag atcagcatct ttttataaaa aaaacacact atcaagtaca attagactat
     2581 ttggcatcca aagggctaaa tttctctcat tctcttgccg caatcaatga tatgatttgg
     2641 tcgacttccg ttcaatatgg ccctacgact ggtttaattt cgagagcgtt gaaaggaaag
     2701 aatttagaaa cattaacaga tgcagatgtg ataactattg ttcaggacta caaaataaat
     2761 catgttgaag atctttttcc atcatccccg acttggtggg atgatttaaa gacacgagcg
     2821 ctaacagaaa agaaagattt attggaatta aatgaattga atctggagtt aaagaatgaa
     2881 gattaaatta tttgcattgc ttattttctt gccgattatc tcttttgcga aagaaccaca
     2941 cgtttgcaat tcctcgatgc agtctcatcc agatattatc gattgcatac aaattagcac
     3001 aaggaaaata gaggattcca ttaaagaaat aattgcgaaa tctggcaacg agtattcaat
     3061 tgatatggcg ttttataata gccagagaaa gtctatttct gaacgatgca gcttatatta
     3121 ttcgatggaa ggtcaacgaa ttgatttgat agaaaatcaa tgcgaatatg atggtgtaga
     3181 acaactaaat aaatttatta aaacctatat gaagacggtt gataattatt aaaatcaaac
     3241 tgtctttggt tatgttaatt gttaatggta ttaacaatat atgctcaatg ctctcaaaga
     3301 aaaatattga cagttctaat cctcaatttt ctgactactt ggtttccgta attaacaggt
     3361 catttagaag tatctcaata agcaagagtt ataggtctgt atgtgaagtg gccttattta
     3421 attttaatta tagtaatggt gtaaaattaa taggattgct ggacggggtt aataattaat
     3481 gactgtctca taaatgttca tcataacgag aaaacggcag tagtataatt cggatgcaac
     3541 atgcatctgg agacgttaag ggttgagtta atagatttga ctctaaatcc ttgataaata
     3601 ttaacttaga ctaaacaaat gggcttgaaa aaacattgct attgctctgc actttatttg
     3661 catttctcat atgtgataat tttgctgtta caaatgaaat gccaagggaa aatattccct
     3721 taggaaaaat gtttgtggta taaaaattaa tgacgaactt gtagagatgt agatagttct
     3781 aaatgtgaat tttattgcgc actatctgta gtatcgcatt catatttgta tgaaattggc
     3841 aaaaaataat agtatttatt gataactcaa tggtaaatac atgc
//
LOCUS       DAZGQL010000212         3784 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.214, whole genome shotgun sequence.
ACCESSION   DAZGQL010000212 DAZGQL010000000
VERSION     DAZGQL010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3784
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.214"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..888)
                     /locus_tag="ACHKIR_005075"
     CDS             complement(<1..888)
                     /locus_tag="ACHKIR_005075"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HFO9992672.1"
                     /translation="MKKFDVAALGSGNIDIFLSIPSLPTRGGKVVGTRLGEQAGGTVA
                     NSACAMGQLGLNVVSVSCVGNDHSASIILDGFKKYHVNCDFVQVIPELIANTAIIFID
                     ELGEKTLVYSPGSDHEWDKEKALQAIAQSRYFYTMPADIEKFRMLAEYSRSQMTKVVV
                     DIEPHIIATPEHLARILHLADIAIFNYDGFIRGYATEPDFTLLHDIQDEYQLDAVVVT
                     LDVRGVIAVKGNEQVKIGSYTIPVIDTTGAGDTFNGAFVYSLIKNMPLIEALKFASAT
                     AAINITALGARGHLPTPSEV"
     gene            complement(893..1891)
                     /locus_tag="ACHKIR_005076"
     CDS             complement(893..1891)
                     /locus_tag="ACHKIR_005076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFO9992673.1"
                     /translation="MNMPARTPVMPLAQTREEINATAASLRHLAAIWGECFSTFQLDK
                     RAVERVCIVGSGDSWTVALCAAAWLGKYTHLFCYALQTWDFLQTDLTRYQKETLIIIL
                     SASGRPSMTVDALCHAVCSNAQVLGVTNCPGTPFCAITENMLYTWANKQGIPTQSSSV
                     TLYALLRLAQKLCPGLTPLQIEDDLEGKFSQINQHWQQKKRHFYQQKEITFLGSGLSW
                     GLAISGSNLLSCGPQIRATALPLEEFYHSLRLHQAGVGQHYILLPATSCESPFYLATQ
                     KAIVEQGATAELISFIPDASESNNLFLVMQWLYEMCWHLSCDYVDAGGQRVSHREK"
     gene            complement(1891..2967)
                     /locus_tag="ACHKIR_005077"
     CDS             complement(1891..2967)
                     /locus_tag="ACHKIR_005077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFO9992674.1"
                     /translation="MNKIEQAVIYNKILGSLACAGMGDALGAATELYSIDEIKAQWGG
                     FLNAFVSPPADTFAGSLNGIAGLITDDSSQMYVFSEGLIEAGFDNFTNNDWLACLLRW
                     ADMQPYANYKGPTTEQIVKALKEGRPTNTIGRIGTSSRQAPNVGTTNGAGMRVAPAGL
                     IWSGKKEKACHLALITCLPSHDTNIAIASACAIAAATSQAMLPEASLTSLLDAAIWGA
                     NYGERLAKQYARCVAGPSIAMRIQLAADIARRANDLESCLREMEGLVGNSVAAHESIP
                     AAIGLLLYCKGEPWETIHACANIGNDTDSIATMAGAIAGAWRGFDALPEDKYAFFRAV
                     NNKDFDIEAIASGLTLLALEAQEK"
     gene            complement(2981..3733)
                     /locus_tag="ACHKIR_005078"
     CDS             complement(2981..3733)
                     /locus_tag="ACHKIR_005078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000591154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO9992675.1"
                     /translation="MIKIYKASKGQLKHREIFDEIKKKVDNGEWKEGKAIPTERELSE
                     YYLSSRTTIRKAIESLKQRGYLHSVHGQGTFVLPSRSRENHLLHSFTDDIKARGGIPA
                     QNILEIGYVPVSDVIRKNLDLDIHVQTVFCIKRVRYMGSTPLGIQTSWLALNDRQEIT
                     QEDLLATGSLYILLEEKLAIKPLEATEIISARLPSPLERQLLELTADDVVLSCTRVTL
                     SVERKPMEYVEMVYPASRYSYKARITKDSFSV"
BASE COUNT          998 a          884 c          808 g         1094 t
ORIGIN      
        1 aacctccgat ggtgtcggca gatgacctct ggcacctaat gcagtaatat ttattgctgc
       61 tgtggcagaa gcaaatttta gcgcttcaat taagggcata ttttttatta agctataaac
      121 aaatgcacca ttaaaagtat ctccagcgcc tgtggtatca ataacgggaa ttgtataact
      181 acctattttt acttgctcat tgcctttgac tgcaattact cccctaacat ccagcgtaac
      241 cacgacagcg tcaagttgat actcgtcttg tatatcatgc agaagtgtaa aatctggctc
      301 tgttgcatag ccacgaataa aaccgtcata attaaatatc gcaatatcag caaggtgtaa
      361 aattctggca aggtgttcag gtgttgcaat gatatgaggc tcaatatcaa cgacaacttt
      421 tgtcatctgg ctacgggaat attcggccag catacgaaat ttctcgatat ctgctggcat
      481 cgtataaaaa taacggcttt gcgcaattgc ctgcagggct ttttctttat cccactcatg
      541 atcactacca ggcgagtaaa ccaacgtttt ttcacctaat tcatcaataa agataatcgc
      601 ggtattggca atcaattccg gaattacctg aacgaaatca caatttacat gatatttttt
      661 aaatccatca agaatgatcg aggctgaatg atcattgccc acacatgaaa cagataccac
      721 attgagaccc agttgtccca tcgcacaggc actgttggcg actgtgccgc cagcttgttc
      781 acccagacgt gtcccgacaa ctttaccgcc acgagtgggg agtgacggga tagataaaaa
      841 aatatctata tttccactgc ctaaagcggc gacatcaaat ttcttcatag tcttatttct
      901 ccctatggct aacgcgttgc ccgcctgcgt cgacataatc acaacttaaa tgccagcaca
      961 tttcatataa ccactgcatg accagaaata aattgttgga ctcactggcg tcaggtataa
     1021 aactgattaa ttctgctgtc gctccctgtt caacaattgc tttttgggtt gcaaggtaaa
     1081 atggggactc gcaggatgtg gctggtagta aaatataatg ctgaccgaca ccagcctgat
     1141 gtaagcgtaa tgaatgataa aattcttcca gtggaagggc tgtcgcacga atctgtgggc
     1201 cgcaagaaag taaattgctc cccgatattg ccagtcccca acttaagcca ctcccaagaa
     1261 acgttatctc tttttgttgg taaaaatggc gcttcttttg ctgccagtgc tgattaatct
     1321 ggctaaattt cccttccaaa tcatcttcaa tctgcagtgg tgttaagcct ggacataatt
     1381 tttgcgctaa gcgcaggagg gcataaagtg tgacgctact actttgcgtt ggaataccct
     1441 gcttattcgc ccatgtatag agcatgtttt ctgttattgc gcaaaatggt gttccagggc
     1501 aattcgtcac acccagcact tgcgcgtttg agcatactgc gtggcagagg gcgtcaacgg
     1561 tcattgatgg tctgccactt gcactgagaa taatgattaa cgtttctttc tgataacggg
     1621 ttaaatcagt ctgaagaaaa tcccacgttt gcaatgcgta acaaaacaga tgtgtgtatt
     1681 tccccagcca ggcggcggca catagtgcca ccgtccagga gtcgcccgat cccactatac
     1741 agacgcgttc aacagcacgt ttatcgagct gaaaagtaga aaaacattca ccccagatag
     1801 ctgccaaatg gcgtaacgat gccgccgtag catttatctc ctcacgagtt tgcgctaatg
     1861 gcataacagg agtacgggct ggcatgttca ttatttctcc tgtgcctcta atgcaagcaa
     1921 tgtcagaccc gatgcgatcg cttcaatatc gaaatcttta ttatttacgg cgcggaaaaa
     1981 ggcatattta tcttcaggga gcgcatcaaa accgcgccat gcccctgcaa ttgcgcctgc
     2041 catcgtagcg atgctatcgg tgtcattgcc aatattggcg caggcatgaa tggtttccca
     2101 gggttctcct ttgcagtaca gcaatagacc gattgccgca ggaatggatt catgggcggc
     2161 aacagagtta ccaaccaaac cttccatttc tcgcagacaa gactccaggt cattggctcg
     2221 tctggcgatg tccgctgcaa gttggatgcg catggcgata gatggccctg cgacacatcg
     2281 tgcatattgt ttggccaggc gttcgccgta attcgctccc cagattgcgg cgtccagtaa
     2341 tgatgtgagc gaagcttctg gtaacatagc ttgtgatgtc gccgcagcga tggcgcaagc
     2401 gctggcaatc gcaatattgg tgtcatgcga aggtaggcaa gttattagcg ccaggtgaca
     2461 cgctttttct ttcttgcctg accagatcaa tcctgcaggg gctacacgca ttcctgctcc
     2521 attcgtggtg ccgacattgg gtgcctgacg agaagatgtt ccaatacgcc caatggtgtt
     2581 tgttggtcta ccttctttta gtgccttgac gatttgttct gttgtggggc ctttataatt
     2641 tgcgtagggc tgcatatcgg cccaacgcag aagacaagct aaccagtcat tattggtgaa
     2701 gttatcaaat ccagcctcaa ttaacccttc tgaaaacaca tacatttggc tggaatcgtc
     2761 ggtgataagt cccgcaatac catttaatga gccagcgaat gtatcggcag gaggagaaac
     2821 aaaggcattt aaaaaacctc cccattgagc ttttatttca tcaatgctgt agagttcagt
     2881 cgctgcgcct aatgcatcgc ccatacctgc gcaggctaaa gaacctaata ttttgttata
     2941 aattacagct tgttctattt tgttcacaat attctctctg ttacacgcta aaactgtctt
     3001 ttgtaattcg tgctttgtag gagtaccggc ttgcgggata caccatctct acatactcca
     3061 ttggtttgcg ttccacggat aacgtgacgc gggtacaact taatacgaca tcatcggcag
     3121 tcagttccag taattgacgc tcaaggggcg agggaagccg ggcagagata atttctgttg
     3181 cttctaaagg tttaatagcg agtttttctt ccagtaaaat atagagcgaa cccgtcgcca
     3241 gcagatcttc ttgggttatt tcttgcctgt catttaatgc aagccatgat gtctgaatac
     3301 ccaggggggt acttcccata tagcgaacgc gttttataca gaatacggtt tggacatgga
     3361 tatccaggtc gagatttttt ctgataacat cactgacagg aacatagcct atctcaagaa
     3421 tattctgtgc tggtatacca cctcgagctt taatatcgtc ggtaaaacta tgcaataaat
     3481 gattttctct actacgagaa ggcaatacaa atgttccttg cccgtggaca ctatgcaggt
     3541 agccgcgttg tttaagactt tctattgcct ttcgaatagt tgttctgcta cttaaataat
     3601 attctgacaa ttccctttct gtcggtatgg ctttgccttc tttccattca ccgttatcta
     3661 cttttttctt tatttcatca aagatttcac gatgctttag ctgaccttta ctggctttat
     3721 aaatttttat catgacaaag cctcatttca agtggttgat accaactcta cattctttaa
     3781 cttt
//
LOCUS       DAZGQL010000213         3702 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.215, whole genome shotgun sequence.
ACCESSION   DAZGQL010000213 DAZGQL010000000
VERSION     DAZGQL010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3702
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.215"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            425..633
                     /locus_tag="ACHKIR_005079"
                     /pseudo
     CDS             425..633
                     /locus_tag="ACHKIR_005079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309369.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            620..809
                     /gene="tnpB"
                     /locus_tag="ACHKIR_005080"
                     /pseudo
     CDS             620..809
                     /gene="tnpB"
                     /locus_tag="ACHKIR_005080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309368.2"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase.; frameshifted; incomplete; partial
                     in the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            complement(812..1000)
                     /locus_tag="ACHKIR_005081"
     CDS             complement(812..1000)
                     /locus_tag="ACHKIR_005081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HFO9992676.1"
                     /translation="MGKRYKITATIIKPGNMPVQWLRYQDSEMTFAECEKLFAPAKEP
                     GRYIRVRVEDFRCEVVKA"
     gene            1535..1676
                     /locus_tag="ACHKIR_005082"
                     /pseudo
     CDS             1535..1676
                     /locus_tag="ACHKIR_005082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014543172.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
     gene            1899..2636
                     /locus_tag="ACHKIR_005083"
     CDS             1899..2636
                     /locus_tag="ACHKIR_005083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFO9992677.1"
                     /translation="MIVCYVILVNRPFETEIAGKKIFFQKNDVLLTDNISQSFFLRHP
                     GNVTELRIDECIVRKYMESADRIQHSDSAVSVPFIKTTFSHIALLHNVMQNLSLQKTL
                     PQQFSEQMFFACLSIFSTHRGFMFLLFRCINSVALKVRYIICSSPSRAWRVKDISNSL
                     YMSESLLKRKLQEEKVSFSQILLDIRMHNARQLISSKLSVNQIAIRCGYASTPYFISV
                     FREYFGITPHQMMINQSGFIESRIAIK"
     gene            3536..>3702
                     /locus_tag="ACHKIR_005084"
     CDS             3536..>3702
                     /locus_tag="ACHKIR_005084"
                     /inference="COORDINATES: protein motif:HMM:NF024418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ESPR-type extended signal peptide-containing
                     protein"
                     /protein_id="HFO9992678.1"
                     /translation="MNKIYALKYSSLTGGLIAVSELSKKVTGKTGRGLMTVSLVLSVT
                     LSALPGKASTV"
BASE COUNT         1040 a          804 c          756 g         1102 t
ORIGIN      
        1 tcagtgttaa ataagattaa taactgtaat tctgcaaaaa aaagaacacc atatgtagta
       61 tgactacgta ttccactctg ttcgttcacg gactggcatc gaagtctgag gtgtactggc
      121 aatagcggat acagtcattt gttctttttt taagcattca tccgatgata cttttcctga
      181 atgctaccgg agtggtattc ccctgccgtg agggagggcg ctgacggtta taaaaatcct
      241 gatatatacc cattcccgta tgacagcgat ggcgtcacat ctctgttcta ccatcttcac
      301 agaaatattt cgttcattag cattccggta ttcgtataac caccttcggt ggagaaagaa
      361 taaacaatcc ctggaatgcg atgagcgctt gaccagaaaa gcatcatatt gcttcatggc
      421 gcatcagcga ccacaggatg ccgcaaaagc cgaaaaacgg accagagcgc ctgcaatcat
      481 tcctcgcctc agtatctgtt gcccatggag tggccgataa cagatcattg tcggatgagc
      541 cttatcctct tccttcccgg tggtatccgg atgtgctgcc agccttctca gttaaccgat
      601 gttttccggg ctttttgcca tactgaggac tgataacgta ttcctggtcg taaaacccgt
      661 caatatgcgc cggggcatcg acaaaactga cacagtacgt ccggaaagaa cttaacgctg
      721 catggcacga caatgctgcc tttgtcttta ccaacaaggc tcgttcccgc ataaaagttc
      781 tacgccggga taaacatggc gtatggctgc gtcaggcctt cactacttca caccggaagt
      841 cttccaccct gacacgaata taccgtccgg gctctttcgc aggtgcaaac agtttttcac
      901 attcggcgaa tgtcatttct gaatcctggt aacgcaacca ctgaaccggc atattaccgg
      961 gtttgattat tgtcgcagtt attttatatc gtttccccat atcgcctctc atcctgacaa
     1021 caaaattacc ggctgagcca tcactccggt aaagcatgcg aaaaaaagtg agcgtctcag
     1081 caatggctca tgcgatgatt ctgtacgggc accgatggca cctcagaata caacaaagat
     1141 atcagagtaa catgtctctc tcgttatttt tcagagttct tgacaccctc cttctgccac
     1201 attgtcaacg ccagtggaga cttctgctgt cagttttttc atccatacag agtgacatta
     1261 attatcctga acaaaacgaa atatcctgaa tatatttatt ttctcctggt gccggagaca
     1321 tgatgatgta caggctcagg tgcggtgtga atgtaaaatg gtatgttaat tattcatttc
     1381 tgaatgcaaa ctgaatgcaa acttattccg aattgaattc tcctgacacc acatgataaa
     1441 aatgaggata taataaaata cttctcttct ggatgtgcca ttacctgccg ttgctgtaat
     1501 acggcgggta ttttttactg acatatcggg aagccagcag acgttcgtag ctacatcccc
     1561 gaaagtggca cactgcaaac tataatacga gtaaaacatc gatcaccgta gctgatgtgt
     1621 tcaacgatcg tgcactagcg ttctgcgatt ctcagggact gcctgttctg agaatcggcg
     1681 agtccgcgat cggatacgat gcgatttcgc agtgactcag ggattgcatc tgaaaaccct
     1741 ccggctatgt cggtaaatat ttatttctat gaaccgtacg attcagatag cattcgcccg
     1801 gccccgtaag aataatctcc ggacaaataa gatttttcct gaaaaagcgt ctagtctttt
     1861 gatatcgttg tttttatgct aatcagggag tatgtgtgat gattgtctgt tacgttattc
     1921 ttgttaaccg tccgtttgaa acagagattg ccgggaaaaa gatatttttt cagaaaaatg
     1981 acgtcctgtt gacagataac atcagccagt cctttttcct gcgccatccc ggtaatgtta
     2041 ctgagctccg gattgatgaa tgtattgtca gaaaatatat ggaaagtgcc gacaggatcc
     2101 agcactctga tagtgctgtt tctgttccgt tcattaaaac aaccttctct cacatcgctc
     2161 tgttacataa tgtcatgcag aatctcagtc tgcaaaagac attgccacaa caattttctg
     2221 agcagatgtt ttttgcctgc ctttccattt tttccacaca cagaggtttc atgtttctgc
     2281 tattcagatg cataaactct gttgcgctta aagtcaggta tattatctgt tccagtccat
     2341 cacgggcatg gagagttaaa gatatcagta attctctgta catgagcgag agtttactga
     2401 aaagaaaact tcaggaggaa aaagtttctt tctcccaaat cctgctggat atacgcatgc
     2461 ataacgcccg acaacttatt tccagtaaac tgtcagtgaa tcaaatcgca atacggtgcg
     2521 gttatgccag cacaccgtat tttatcagcg ttttcagaga atattttggc ataacacctc
     2581 atcagatgat gattaatcag tccggattta ttgagagcag aatcgcaatt aagtaaagta
     2641 tgtcagtcag cgcataataa aagtattctt atgcttcatt atcagtttct gtttatcact
     2701 ccggagcact gtagtaacta cggccccgga aaacagatgg taatgagttg actgactgat
     2761 gatattgtgg cgacattttt acggatgcga gcaaagaatg tattggtccg tttgattatg
     2821 cagtgattaa caaaactccg ggaagatgaa tgagtatacc tctcctgcaa tccgtttcct
     2881 gagaaaacag gtggtaacac aatattctta atatgttact gtttcaccac acaaccaggt
     2941 ttacggttgt gtggtgaact atagtaatcc cgggtaacaa tccggagctg ttccttttat
     3001 actcttcagg ctgtgtactc atccacgcca tccgtttttc agtctcagtg tgtgaataat
     3061 aaattacact ttgaactgaa cgccagtcag gaccatgtcg tatttccggg tggatgagca
     3121 tatatcagaa actacaataa ctacagataa cggttttcag taattctgtc ataacttgcc
     3181 ggaatgcaat gtttttgtga catggttctt attttttata cacaggcagg ttttgtctgg
     3241 atggaatgga taatgaactc ttatcattat aaggcggtaa cctctttttt atgtgattaa
     3301 ctttcattat taccacttct gtttatcgat tcagctctgg agatacagaa aaattcagtg
     3361 caggtgtata aatcatcacc atgcggaata cataaaatat gttaatattt gttttccgca
     3421 ggaaaatctt aagtgtggac aggtatttat gataccattt aaaatagatt cagcactcac
     3481 tctttccgga cagataatac atttcaatac catataatta tataacagga attatatgaa
     3541 taaaatatac gcgcttaaat atagctccct tactggtggg cttatagctg tgtcagaatt
     3601 aagtaagaag gtcacaggaa aaaccggcag aggattaatg acggtttccc tggtattatc
     3661 agtgactctt tctgctttgc cgggtaaagc atcaacggtc ag
//
LOCUS       DAZGQL010000214         3694 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.216, whole genome shotgun sequence.
ACCESSION   DAZGQL010000214 DAZGQL010000000
VERSION     DAZGQL010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3694
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.216"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..238
                     /locus_tag="ACHKIR_005085"
     CDS             <1..238
                     /locus_tag="ACHKIR_005085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014728563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HFO9992679.1"
                     /translation="TDMTLDMLKQVDWVQVLTSVAKTVGVILVAVGVGGLVVTLGIPE
                     AIIGAFLLIVRLAISAWGTLATILGTGTAVLAVG"
     gene            251..1465
                     /locus_tag="ACHKIR_005086"
     CDS             251..1465
                     /locus_tag="ACHKIR_005086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3396 domain-containing protein"
                     /protein_id="HFO9992680.1"
                     /translation="MELHDFELNYIEKWLPEASVKYKDGSPAVNPGLIITVFFKDGHT
                     PEVRRRMVECVDRFYGEFKPWLKKTITKNWVGITDNNYAKKRQEILDSTPEEIFSWYL
                     SSAEKDFLAPDYSILIMGKRIFHNDNNRSVIKLTLPLSLLKETDGKQRYEAWLMWLCD
                     TFSVESGYAGLSFALPYARERMFPCEFALAQRFSGVMVDSLGTLEGGEAVEGLKGACW
                     YTILGTPWLEKLGGAERVKHRLAKTPEISLLPYNNGVVLKAGELPPPLGEMKTEGLSP
                     LLVKVNQVIRSIRYDGRNGLHFYSEYEGLQFEEASSMAWFARFDEASALLDKEEMGKS
                     CAPERIKCWTDEIAPHAGQWAAMVNGTTEYIQTREGQKMPPFEDKHGKQHRACWKLLK
                     RDDNGSVYTLPE"
     gene            1479..1832
                     /locus_tag="ACHKIR_005087"
     CDS             1479..1832
                     /locus_tag="ACHKIR_005087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2345 domain-containing protein"
                     /protein_id="HFO9992681.1"
                     /translation="MTVQAGERLGLFARTGQLSLKSGEGAVDVQAQNGSMRLFAEKKL
                     TLSSASDISFAGKKRITLIGGGSYLRLEAGKIEYGTMATYLRRTKRTMVAAPATMPLP
                     IPLLLDLYPPLNGQI"
     gene            2161..2616
                     /gene="tssE"
                     /locus_tag="ACHKIR_005088"
     CDS             2161..2616
                     /gene="tssE"
                     /locus_tag="ACHKIR_005088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000018100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFO9992682.1"
                     /translation="MSHHSPSLYETLYGNFTGGLDLHQVCEENQVIVSVLDNMQRILN
                     TRAGSLKHLPDYGLPDMTTILQGMPGTAHQLMRVLSDVLLKYEPRIKRVDVTMQEQTQ
                     PGELHYVIDAELKDAGLVRYGTTFMPEGRVLLRHLKQQQFVDNSSYYHV"
     gene            2684..2989
                     /locus_tag="ACHKIR_005089"
     CDS             2684..2989
                     /locus_tag="ACHKIR_005089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001604127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992683.1"
                     /translation="MNPLFNDIQMRLFYLNHSPYSWHWNVRFRPQEAVYIGSDTCHIT
                     ITCNQSGFHLTRDGQRVFTERYIRNLNELLPVLKRRWDVTPAIIRAVEYLSRVPVSH"
     gene            3044..>3694
                     /locus_tag="ACHKIR_005090"
     CDS             3044..>3694
                     /locus_tag="ACHKIR_005090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ImpA family N-terminal
                     domain-containing protein"
                     /protein_id="HFO9992684.1"
                     /translation="MTSEVKLKTGGDPRSFPDYAALRDEISKLTHPARPDVDWRYVET
                     LCLRLYEHNGVELQTASWYTIARMHTTGLSGLNEGLALIVALTRHHWSVMWPLNTHAR
                     LEIITGLFNRLQKTLRAMPPDDRDNLPLLYQTETFLKALSDTLARHELKQSSKVALPE
                     TMVKGYITRLENQPMQGEVSSPVTLPARALRSDAPDVQGHQPLPHSRLVYIVSETKA"
BASE COUNT          938 a          846 c          985 g          925 t
ORIGIN      
        1 caccgatatg acgcttgata tgctgaaaca ggtggactgg gtacaggttt taaccagtgt
       61 cgcgaaaacg gtcggcgtta ttctggttgc tgtgggtgtc ggcggactgg tcgtaacgct
      121 gggtatcccg gaagccatta ttggcgcgtt tttactgatt gttcgtctgg caataagcgc
      181 ctggggaaca ctggcgacga ttctcggtac gggtacggcg gtactggcag ttggttaaac
      241 aggagcaggt atggaattgc atgattttga gctgaactac attgagaaat ggctgccgga
      301 agcctccgtg aaatacaaag acggcagtcc ggcagtcaat cccggtctga ttatcaccgt
      361 cttttttaag gacggacata cgccagaagt ccgccgcagg atggtggagt gcgtggatcg
      421 cttctatggg gagtttaaac cctggctgaa aaaaacgatc accaaaaact gggttggcat
      481 cactgataat aactacgcca aaaagcggca ggagatcctc gattcaacgc ctgaagagat
      541 tttttcctgg tatctgagta gcgcagagaa ggatttttta gcccccgatt acagtatttt
      601 aattatgggg aaaagaattt ttcataatga caataatcgt tcagtcatta agttaacact
      661 cccgttgtca ctgctgaaag aaactgatgg aaagcagcgt tatgaagcct ggctgatgtg
      721 gttgtgtgac accttttcgg tggaaagtgg ttatgcaggg ttgtcgtttg cacttcctta
      781 tgcccgtgag cgaatgttcc cctgtgaatt cgccctggcg cagcgttttt ccggtgtgat
      841 ggtggattct cttgggacgc ttgaaggcgg tgaagccgtt gaggggctga aaggtgcctg
      901 ctggtacacc attctcggta cgccgtggct ggaaaaactg gggggggcag agagagtaaa
      961 acatcgttta gctaaaacgc ctgaaattag cctgctaccc tataacaatg gtgtcgtact
     1021 taaagccggt gaactccccc cgccactggg agagatgaaa acagaaggtt tatcgccgct
     1081 gctggtgaag gtgaaccagg tcattcgttc cattcgctat gatggacgca atggtctgca
     1141 tttttactca gaatatgaag gacttcagtt tgaagaagcg tcttcaatgg catggtttgc
     1201 ccgttttgac gaagccagtg ccttgctcga taaagaagaa atggggaaat cttgcgcccc
     1261 cgaacgcatt aaatgctgga ccgatgaaat agcgccccat gccggacaat gggccgcgat
     1321 ggttaatggc accaccgagt acatccagac ccgcgaaggg cagaaaatgc ccccgtttga
     1381 ggataagcac ggtaagcagc acagggcttg ctggaaacta ctgaaacgag atgacaatgg
     1441 tagcgtttat acactacctg agtaaggtcg tgtgctgaat gaccgtgcag gccggagaga
     1501 ggctggggct ttttgcccgc accggtcaac ttagcctgaa atccggggaa ggggcagtgg
     1561 atgttcaggc gcaaaacggc agcatgcggt tgtttgctga gaagaagctg acgctgagct
     1621 cagcgagtga catctccttt gccgggaaaa aacgcatcac gctgattggc gggggaagtt
     1681 acctacggct ggaggcgggg aaaatagagt acggtacgat ggcgacgtat ctgcggcgga
     1741 cgaaacggac gatggtagca gccccggcaa caatgccgct ccccataccg cttctcctgg
     1801 acctgtatcc accacttaat ggccaaattt gaattgaatg tctcctcaat gccatcaaag
     1861 tcaacgatgc tatcgttcag ggagcgtagt caatggatgt cacaaaaatg atgcaggtgc
     1921 tacaggattt gtttttatgc tgctaaatgt acagcgactt cgtgatatgg ggttgttggt
     1981 gttttttata ataatgcttt ttacggaatc aaaaaaacag acaacatatt aattatttga
     2041 gaaagcagcg tcaaaaagga aaagttaaaa tgtcgaatcg attttttact ccggtaatct
     2101 tctttccagt gtgttgattg aactatttcc aagattaaat gattatgaga agaagtcgtg
     2161 atgtctcatc atagcccctc cctttacgaa accttatacg gaaattttac cggcggcctc
     2221 gacctccatc aggtctgtga agaaaatcag gtcatcgtgt cggtgctcga taacatgcag
     2281 cgtattctga atacccgggc cggaagcctt aaacatcttc cggactatgg gctgccggat
     2341 atgacaacca ttctgcaggg aatgccgggt acggcgcatc aactgatgcg cgtgctttca
     2401 gatgttctgc tgaagtatga gccacgtatt aagcgtgtcg acgtgacgat gcaggaacag
     2461 actcagcctg gtgagttgca ttatgtcatt gatgcagaac ttaaagatgc cgggctggtt
     2521 cgttacggaa caacatttat gccggagggc agagtgttgc tgcgccatct taagcaacag
     2581 cagtttgtgg acaattcatc ttattatcat gtctgatttt tttatccggg ctgcccctgt
     2641 gtgggtggtc cggatgaaaa atatattttt tgtgattttg attatgaacc cgttatttaa
     2701 cgatattcag atgcgtcttt tttatctgaa ccactctccg tactcctggc actggaatgt
     2761 cagattcaga cctcaggaag ccgtatatat tggcagcgat acctgtcata tcaccattac
     2821 ctgtaatcag tccggttttc atttaacccg ggacggacaa cgggtattca ctgagcgtta
     2881 tatcagaaac cttaatgagc tgttaccagt tctgaaacgg cggtgggatg tgacaccggc
     2941 aatcatccgt gcagtggaat acctttcccg ggtcccggta tcacactgac attctttccg
     3001 taactgttaa tcatgattta atacagcaac actgaatctg ccgatgactt ctgaagtgaa
     3061 actgaaaaca ggtggtgacc cccggtcatt cccggattat gctgccctgc gcgatgaaat
     3121 cagcaaactg acccacccgg cacgcccgga tgtggactgg cgttatgtgg aaacgctctg
     3181 cctgcgcctg tatgaacaca atggcgtgga attacagaca gcatcctggt acaccattgc
     3241 ccggatgcat acaacagggt taagtggcct gaatgaaggg ctggctctga ttgtggcgct
     3301 gacccggcat cactggtcag tgatgtggcc gctgaatacc cacgcccggc tggaaattat
     3361 taccggtctg ttcaaccgtc tgcaaaaaac gctccgggcc atgccaccgg acgaccgtga
     3421 caacctgccc ctgctgtatc agaccgaaac attcctgaag gcactgagtg atacactggc
     3481 ccggcacgaa cttaaacaga gcagtaaagt ggctttaccg gaaaccatgg tgaagggata
     3541 tatcacccgt ctggaaaatc agccgatgca gggcgaagtg tcgtctccgg taacccttcc
     3601 tgctcgggca ctccggtctg atgctccgga tgtacaggga catcagcccc tgccgcactc
     3661 ccgtctggtg tatattgttt ctgaaacaaa agca
//
LOCUS       DAZGQL010000215         3635 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.217, whole genome shotgun sequence.
ACCESSION   DAZGQL010000215 DAZGQL010000000
VERSION     DAZGQL010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3635
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.217"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(144..1463)
                     /locus_tag="ACHKIR_005091"
     CDS             complement(144..1463)
                     /locus_tag="ACHKIR_005091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HFO9992685.1"
                     /translation="MDICSRNEKLAIRRPAILVALALLLCSCKSTPPESMVTPPTGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMVDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPEVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            complement(1594..3129)
                     /gene="gadC"
                     /locus_tag="ACHKIR_005092"
     CDS             complement(1594..3129)
                     /gene="gadC"
                     /locus_tag="ACHKIR_005092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HFO9992686.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKSKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            complement(3285..>3635)
                     /locus_tag="ACHKIR_005093"
     CDS             complement(3285..>3635)
                     /locus_tag="ACHKIR_005093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFO9992687.1"
                     /translation="AYLADEIAKLGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTLYD
                     LSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSDHP
                     KLQGIAQQNSFKHT"
BASE COUNT          949 a          892 c          912 g          882 t
ORIGIN      
        1 taggggattt atgactgttt aactaagtgt ggttaattgc gtagttaatt tgacttaagt
       61 aagcatgatt actagtggga tatttcaaga gggtaacaag ccggggggaa agcaccggct
      121 tgttacaaag taagaatggg agtttaactg ccccaacgac tttgcagata gctgaccgct
      181 tgttgagtct gtggcttatt cagatagtcc tcacggaaca agattgtgcc gctaatttct
      241 ggcacggcat cgttaagatc gagctgcttt ttcagttccg gtacgccgcc gttaatcatc
      301 cagtctggct ctatctttga aggttcaccc actttataga aggcgatacc gatatacagg
      361 cgggtcctgg tcggtttaac gacatccgcc caccattttg ccaaaacgtc ataacgtgcg
      421 gcactccgtg agaacggcca gtaaatttgg ggagcaatgt aatccagcaa tccttgttcc
      481 acccatcgac gggtgtcagc gtaggattcg tcataggctg ccgcgcctcg ggtatcggaa
      541 ccgagcggat cgtgtgatcg gttacgccac acgcctgccg ggctaacgcc aaattcgact
      601 tccggcttaa tgcttttaat agtgtgcgat acctttgcaa ttaactgctg agtattgttg
      661 cgccgccagt ctgcttttga cgcaaatgcg cctccgtatt tgcggtacgt ttcgttatca
      721 tttagccgtg aacccggtga ctcagtatag aaatagtcgt caaactgcac gccatctacc
      781 ggatagcggg aaaccacttc cgcgactatt gatgtgatcc agtcctgaac ttcagggatg
      841 cccgggtcga ggacaaagcg atcgccggac gttctgatcc agtcgcggtg ttgcacatag
      901 acgctcgctg gttgttgaga cagagtgcta ttcagttccc tgatagtacc gggcttcgta
      961 ttaaccgata cgcgataggg gttaaaccag gcgtgtactt tcatcccacg cttgtgggct
     1021 tcgtcgagca taaattgcag cggatcgtaa cctggatttt caccaatctt tccggtcata
     1081 agatcggacc acggcaaaat ttttgatggc cacagggcgg taccgtccgg cttgacctgg
     1141 aaaaagaccg tgttgatgcc gagacgttgc agatgatcca gtttgtcgac catcgcctgt
     1201 tgttgtacac gggcccggct ggtggggtta ctaatgttaa ccgaggaaac cggtggccag
     1261 tcgaggcgag acactgtggc cagccagatg ccacgcatcg gttgtgacga ttgttgcgtc
     1321 gtagctggtg gcttcgaacc tgttggtggt gtcaccattg actctggagg cgtgcttttg
     1381 caactacaca gtaaaagtgc cagtgcaacc agtatcgctg gtcttctaat cgctaatttc
     1441 tcgtttcggg agcagatatc catatgtgct ggtttccggt aaacagatgt gcgctcattc
     1501 tcgtacttat cccggttaag tcaatacgac aacaagcacg aaaaagggag cgatgaatta
     1561 tcgctccctt gtcttataac cattcagaca tggttagtgt ttcttgtcat tcatcacaat
     1621 atagtgtggt gaacgtgcac gcgggtgcag gaagaagtga ccttttggtg cgttctgact
     1681 gttgattggc tccagagtga cgccggtatt cgctttgctt ttacgatcat gaacagcata
     1741 gagaataaag ggcagggcaa gtaccaccag gaaactcacg accagtaatt caacatacat
     1801 atcggtagag tcaccctgga tgttatccgg cggcaggaag gaaacaataa acgccataat
     1861 tgaagtcagc agaccgacaa tagccacgac cagtttcacc cctttaccac cagggatatt
     1921 aaatgtgcgt tttaagtcag gatgtttaag aaccaacaca atgtagccaa taaacagcat
     1981 gaaataagca cacagataaa tcaccaccgt cagcgccagt gcgatcagga aggacatgtt
     2041 gttaccgcca ccggtattgg tgaggatgat caacgcgata gaagtaatca ccaactgcga
     2101 aatgaccagc gttaccggta cgccattttt gttcatttta gcgaatgccg ctggcagcag
     2161 gtttttctgc gctgtgacat acatcccgcg agaaggacca acaatccagg aagcgatttc
     2221 cgccagaaca cccagcaaca gcagtgcgga gatcacgcga accgtccact caatttccgg
     2281 tgccacatgt gacatcagaa cggtaaaggt ttgcattacc cctgcggaga ggttgatttc
     2341 attacccgga atgaccatcg caatagacaa accgccaaca gagcttaagc agattgccgc
     2401 caccatcagc agtaacatag ccagtggata gtcgcgcccg ggattgctca tttcattgac
     2461 gtgggttgcg gaagcttcaa cgcccatata actcaaaatg aaggcaacaa atacaaccag
     2521 ggtgcccact ttagagaagt cagggaagaa ggtcttcgaa tccatttcga tagcaacggg
     2581 ggcaccggag tgcagataaa tagccgctaa tgcgatcaaa ataaatgcag gtaacaggat
     2641 accggcgaag aagccaactt tagcaattcg cgccgtgtat ttcgtgccac caaactgcgt
     2701 taatgccagc gcccaaagaa tgatgagtgc tgcaatagtt ttggtaatgg ggtcttcatt
     2761 cagcgctggc catttcagga tgtaggagag tgcccctaac acgaaataga gcatcggaat
     2821 aaaaccaatg gcgatttgca gatagccaaa tgagatcgct gcaaatcccc atctcggccc
     2881 cagagtattt gatacccagg cgaagacgcc accttcttcc cagccgtcga cggtagccat
     2941 ttccgcagca caaagtccca cgggaataaa ccataaaatc ccgcctaata gcaggaagaa
     3001 gactaatgaa aagcccgatg ttgcgaaggt agggtattca taaacagcca ttaccatcga
     3061 tgccgttatg gcaaaaaatc cgagtaatgt gagctgctta gctttacctg tctgtactga
     3121 tgtagccata ttatccccct aaaacggtat tcctgtcgga accgcacctg ttttgttctg
     3181 aagcgtattc agaacaatat tttccgttgc taatgccagt gaacagactt tggaaattgt
     3241 cccgaaacgg cttcgtttcg ggacaccgtt accgttaaac gttatcaggt gtgtttaaag
     3301 ctgttctgct gggcaatacc ctgcagtttc gggtggtcgc tgagatattt cagggaggct
     3361 ttgtagtctt ccagcaacag ttcagcaaag tccatttcga agccgcgacg gcacataatg
     3421 cgcatcacca cgatgtcggt ggcttcaccg cctagagtga aagccggaac ctgccagccg
     3481 cgcagacgca aacgttcaga gaggtcatac agggtgtatc ccggatcttc accatctttc
     3541 agtttgaagc aaaccgccgg gatgccttcg tccgggcgac ccgtacagat gaactcatac
     3601 ggccccagtt tggcgatttc atccgccaga taagc
//
LOCUS       DAZGQL010000216         3551 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.218, whole genome shotgun sequence.
ACCESSION   DAZGQL010000216 DAZGQL010000000
VERSION     DAZGQL010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3551
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.218"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(273..2315)
                     /locus_tag="ACHKIR_005094"
     CDS             complement(273..2315)
                     /locus_tag="ACHKIR_005094"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001175889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFO9992688.1"
                     /translation="MQLFLCEKPSQAKDIARELALSKRGDGYIGDDNIAVTWAIGHLL
                     ELAEPEAYGEQFGSPWRSDVLPLIPEQWQMVVKPKVKKQLIVIRGLLKRASEVVIATD
                     ADREGEVIARELLEYCQYRGRVRRLWLSALDEASIRKALNNILPGEQTALLSEAGKGR
                     ARADWLTGMNLTRLYTLKARERGISGVLSVGRVQTPTLAMVVRRDQEISTFVPVSWWQ
                     VIVWLEKEGLRFRSNWVAAEQYCDDEGRCVNVQAAGAVLGLCRQQSSAVVQDVVQKRE
                     KTPPPLCFSLGTLQEACSRKFGMGAQTVLDIAQSLYETHKATTYPRTDCGYLPASMHE
                     ESSEVLEAVGRSDPSVLPQLAELESGRVSRIWNDKKITAHHAIIPTRQVFDLSSLNGD
                     ELKVYQLIRQHYLAQFLPEQEADVTEATFVIGGQLFRSRGRVNVIIGWKQLFPPENHA
                     DEEKPDSDESGNILPPLIKGELCALDGAEQKELKTVPPRPFTEGSLITAMKNASSGVT
                     EPALKKILRENAGLGTEATRAGILETLFARNYIERAGKHIRSTQLGRELVAALPDSLT
                     NPGMTALWEQALDDVARGKISLEAFMEKQHQWIRHLVLSGRNQTVNLSVESGPSCPLC
                     NGPTVKRKGKSGFFWGCSRYPDCRGIAGEQGNSRGRKRKINFSHKQKSGDVGDKLN"
     gene            complement(2318..3022)
                     /locus_tag="ACHKIR_005095"
     CDS             complement(2318..3022)
                     /locus_tag="ACHKIR_005095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL_4669 family integrating conjugative element
                     protein"
                     /protein_id="HFO9992689.1"
                     /translation="MPQEKTGEVRKSEQVGALQSSLTIALHTRYAIRLWEGRRNNQKE
                     EKQEKRPDIISMPRAIAFAGQATRDSARDNPYADMMLVRLENALTKATETIEKHLAWL
                     DGILKNLPGQISLSDIKSSSPVNIGVYSSSPVGYRCVWLLIGYDRLVMKVFQVFHYGL
                     ISRTKRDELLNQGGYAVRRIYGIIQNYKSVSVTRADILSGTARGKEAVNRMGEPDPDV
                     MSGKKRSVYSPPVKAG"
     gene            3068..>3551
                     /locus_tag="ACHKIR_005096"
     CDS             3068..>3551
                     /locus_tag="ACHKIR_005096"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992690.1"
                     /translation="MRITDNKKTNQAEYTFNAAYFLIAVATGNTAHFLTSVATGSTVH
                     FSTSAATGSTVRFLTSAATGSTAHFLISAATGSTVRFLISAATGSTVRFLTSAATGST
                     VHFLTSAATGSTAHFLIPAATGRTVHFLTSSATGSTVHFLTSAATGSTAHFLIPAATG
                     S"
BASE COUNT          826 a         1053 c          749 g          923 t
ORIGIN      
        1 agcgacgtta tgtgcccgga cagactgaaa tcactgatgg aaaaagtcaa cagctaacca
       61 aaaacaaaaa agcaccggca agccggtgct tcagggggat accaaatccc ggtgttgatt
      121 ctcatcttct ccggagagag cgactcccgg cacggttttc acggagtgca tgtgccttgt
      181 aaccccgccg tgtgcataac gggttaccgg acctctcagg gctgtcagcc ccactgtggt
      241 caacagggtg ataccagaac tcgcccgcaa ttttaattca gtttgtctcc gacatcacca
      301 gatttttgtt tatgagaaaa attaattttt cttttcctgc ccctgctgtt tccctgctca
      361 ccagcaattc ccctgcagtc gggataacgg gagcatcccc agaaaaaacc gctttttccc
      421 ttccgtttca ccgtcggacc gttacagagt ggacatgaag gacctgattc cactgacaga
      481 ttaaccgtct ggttacgtcc ggataacacc aggtgcctga tccactgatg ctgtttctcc
      541 ataaacgcct ccagtgagat tttcccccgg gcaacatcat ccagcgcctg ttcccataaa
      601 gccgtcatac ccggatttgt cagggagtcc ggcaatgccg ccaccagttc tcttcccagt
      661 tgcgtggaac ggatatgttt tcccgctctc tcaatatagt tacgcgcaaa taacgtttcc
      721 aggatccccg cgcgtgtggc ttccgttccc agtcccgcat tctccctcag gatctttttc
      781 agcgccggct ccgttacacc agacgaagca ttcttcatcg cagtaataag actgccttca
      841 gtaaaaggac ggggaggtac ggttttcagc tccttctgtt cagcaccatc cagagcacag
      901 agttctccct ttatcaaagg gggcaatatg tttccacttt catcactgtc aggcttttct
      961 tcatctgcat gattttccgg tggaaataac tgtttccacc cgataatcac attaactctt
     1021 ccgcgggaac ggaataactg ccccccaatc acaaatgttg cctcagtaac atcagcttcc
     1081 tgctccggca gaaactgtgc cagatagtgc tgacgaatca gttgatatac cttcagttca
     1141 tcaccgttca gtgagctgag gtcaaaaacc tgccgggtcg gaataatggc atggtgagcc
     1201 gtaatttttt tatcattcca gatacgggat acccgtcctg attccagttc agccagttgt
     1261 ggaagcacgg acggatctga acgcccgaca gcctcaagca cttcagaact ctcctcatgc
     1321 atcgaggcag gcagataacc acagtctgtc ctgggataag ttgttgcttt gtgagtctca
     1381 taaagggact gtgcgatatc cagaacggtc tgagccccca tgccaaactt ccgggaacag
     1441 gcttcctgca acgttcccag cgaaaaacac aaaggtggcg gtgttttctc cctcttctgt
     1501 acaacatcct gtacaacagc agaggactgc tgacggcaca gaccgagaac agcaccggcc
     1561 gcctgcacat tcacacaacg tccctcatca tcgcagtact gctcagctgc cacccagtta
     1621 ctgcggaaac ggagcccctc cttttccagc cagacaatca cctgccacca ggaaaccggc
     1681 acaaatgtgg agatctcctg atcccgtctg accaccattg ccagcgtggg tgtctgcacc
     1741 cggcccactg acaaaacccc agaaattcca cgttcccgtg ccttcagggt atacaaccgg
     1801 gtcagattca tccctgtcag ccagtcagcc cgggcacgcc ccttaccggc ttcactgaga
     1861 agcgcagtct gttcgcccgg gagaatatta ttcagcgcct tcctgatact ggcctcatcc
     1921 agtgcagata accacagacg cctgacacgc cctctgtact gacagtactc cagcaactct
     1981 ctggctatca cttctccttc acgatcggca tctgtggcaa taaccacttc agaagcgcgt
     2041 tttaacagcc cccggatcac aataagttgt tttttcacct tcggcttcac caccatctgc
     2101 cattgttccg gtatcagcgg caacacatca gaacgccatg gggaaccgaa ctgttcaccg
     2161 taagcttccg gctcagccag ttccagcagg tgtcctatcg cccaggtgac agcaatattg
     2221 tcatctccga tgtatccgtc gccccgctta ctgagcgcca gttcacgggc gatatctttc
     2281 gcctgagaag gtttttcaca cagaaagagt tgcatactca ccccgcttta accggtggtg
     2341 aataaacaga acgtttctta ccgctcatca cgtcaggatc cggctccccc atgcggttaa
     2401 ccgcctcctt tccacgggct gtgccggaca aaatatcagc acgtgtaaca ctgactgatt
     2461 tataattttg aataatcccg taaatcctgc gtaccgcata ccctccctga ttcagcaatt
     2521 catcccgttt tgtgcgggaa ataagtccgt aatgaaatac ctgaaatacc ttcatgacca
     2581 gtcgatcata tccgatcaga agccatacac atcgataacc gaccggagaa ctactgtaca
     2641 cgccaatatt taccggcgaa gaggatttaa tatcagaaag ggagatctgt cccggcaggt
     2701 ttttaaggat cccgtcaagc cacgcaagat gcttttcaat tgtttcagtt gccttcgtca
     2761 gggcattttc cagcctgact aacatcatat ctgcataagg attatccctg gctgaatctc
     2821 tggttgcctg acccgcaaac gcaatagccc tgggcatact gatgatgtca ggccgctttt
     2881 cctgtttttc ttccttctga ttattcctgc gtccctccca gagacggatc gcatatcggg
     2941 tatgaagggc aatagtcagc gatgactgca gagcaccaac ttgttctgat tttctgactt
     3001 ctccggtttt ctcctggggc attgtgttta ctccgaatga tttcatgacc agaagatagt
     3061 ccgcaggttg cgtattaccg acaacaaaaa aacaaatcag gcagaatata catttaacgc
     3121 cgcttatttt ttaatcgctg ttgccactgg caacaccgct cactttttaa cctctgttgc
     3181 cactggcagc actgttcact tttcaacctc tgctgccact ggcagcactg ttcgcttttt
     3241 aacctctgct gctactggca gcactgctca ttttttaatc tctgctgcca ctggcagcac
     3301 tgttcgtttt ttaatctctg ctgccactgg cagcactgtt cgctttttaa cctctgctgc
     3361 cactggcagc actgttcact ttttgacctc tgctgccact ggcagcactg ctcatttttt
     3421 aatccctgct gccactggca gaactgttca ctttttaacc tcttctgcca ctggcagcac
     3481 tgttcacttt ttgacctctg ctgccactgg cagcactgct cattttttaa tccctgctgc
     3541 cactggcagc a
//
LOCUS       DAZGQL010000217         3495 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.219, whole genome shotgun sequence.
ACCESSION   DAZGQL010000217 DAZGQL010000000
VERSION     DAZGQL010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3495
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.219"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..220
                     /locus_tag="ACHKIR_005097"
     CDS             <1..220
                     /locus_tag="ACHKIR_005097"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992691.1"
                     /translation="KKTTHPNHLIIRFLTDFLNNKISFAGGWSHCVTPTTITSVFSRT
                     VVNHKIGTHAGGAGPHDFLFDESENGER"
     gene            complement(1385..1579)
                     /locus_tag="ACHKIR_005098"
     CDS             complement(1385..1579)
                     /locus_tag="ACHKIR_005098"
                     /inference="COORDINATES: protein motif:HMM:NF033650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HFO9992692.1"
                     /translation="MNQDKAIYLENKYFSPANEAVRLEREGDYTGAWKKWHDALHYAK
                     GENIDWCIARAEYCKKTGRF"
     gene            complement(2153..3169)
                     /locus_tag="ACHKIR_005099"
     CDS             complement(2153..3169)
                     /locus_tag="ACHKIR_005099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020240588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HFO9992693.1"
                     /translation="MKNPDSPVLSLRDYSTRDTKWDSDRVMADRVAQIYESDSMFSSR
                     GERMFDCSRRLLFAPKVSRLTGEMKLALRKGEFCHVPFCPVCSRRRSLRWMRRLWEAL
                     PKLLVERPAARWLFMTLTVKNPPVENTRETLIRMNAAWKRLSDRKEFRPVLGWLRTTE
                     ITYGRVPGCCHPHFHVLMMVPPSMLSGNGYVKHARWVEIWSECLRVDYEAGVDIRVVK
                     PKQGWKQPDGVTLPDMHRAALESGVIETMKYTVKSSEVVRDPAWFLELARQTYGLRMV
                     ATGGRLKEVLKVDKPETDEDLVGADIPAEPDEFEEQAFWLAFDWGRTEKRYKRNPEAD
                     RMKD"
BASE COUNT          906 a          937 c          741 g          911 t
ORIGIN      
        1 aaaaaaaaca acacatccaa accacctgat catcaggttt ctcaccgact ttctgaacaa
       61 caaaatcagc tttgcaggtg gttggtccca ctgcgtgaca ccaaccacca tcaccagtgt
      121 tttttctcgt accgtcgtca atcacaaaat cggaacccac gccggaggcg cgggacccca
      181 cgattttctg tttgacgagt cagaaaacgg cgaacgctga cagatttcaa tctggctgcg
      241 tgagacgttg gcggggtgtc ggggcgcagc cctgaccaag tggtaatcgt atagccgtgc
      301 gtgcgcggtt atacgattac acatcctgtc ccggaatcag gattgttttt cgcgcacaac
      361 tgcggcaaat tcaccccgtt gcggttgcaa cgatgggaat atcaaaacta cgaaaattat
      421 tcaggcattt gctacccctc tggccctggc ctccacctgt tccggggggg atttttttgt
      481 tgcctctcat gacgaactgc ggcaaatttc tgacgttgca aactgcaaca cataaaacac
      541 acagaaaaac tcacaattgc catgtcctca ttgacacccc cttgccatat agggggattt
      601 tttcgcctgt cctgattgtc ttttgctgat agttacggca gattggaaaa gagcgcattt
      661 gtggtgctct ctccaaaata atatttctca ttactcaaac tctgatggct tccccctgta
      721 atccaggggg atttttttaa tttagcgcta tgcttcgtaa ttcattcaga atacagacag
      781 tattgttaat cagtttttca tcatgaacgg tgtgttcagc aataagagta ttaaactcaa
      841 taatcaggct gtaactctct ctgtatacat catctctgtt aaattcgttc gctctgtatt
      901 ttgaataaat aataatgcct ttgtcctgta tactctgaat ttgtttaatt acatcaatac
      961 tcataaaaac atccaacaag aaccgactgt aggtcaccgg gcaaacgagc gaaatgctag
     1021 cattttgcgg cgtttgccct atcctgcatc agaacgctaa atacaggaat ctctttatga
     1081 cgattcctcc atttcaagat tttttatgtg gcgtctacta catgcgatcc gttggtgaac
     1141 cgttagttag cctttagatg gaccttagat ggaccttaga tggacactag ttagatattt
     1201 actgatttag atgaacctta aatggatctt agatggatat tagatggatc ttagttagac
     1261 attatgtaga taggaaatat tatggggcaa tcctagaggc acgtgaccga ctgtatacaa
     1321 aatgctggcg cattttgata cgtcgacgtg ccaaagggga cgcccctttg aaaccccgcc
     1381 agaattaaaa ccggcctgtc tttttacaat attcagctct ggctatacac cagtctatat
     1441 tttcgccttt ggcataatga agcgcatcat gccatttttt ccatgctcca gtgtaatcgc
     1501 cttcacgctc cagtctgaca gcttcgtttg cgggtgaaaa atatttattt tctaaataaa
     1561 ttgctttgtc ctgattcata ctcgtaattt atatttttat ttgaattaaa aaagagcggc
     1621 ctgtcgggtt aatacatact ggcatgactg ttgatatatc agcccaactg cacgctcgta
     1681 atcgttttaa cgtgattttc gcaacatggc aatccgggtt catctgtccg gtaatcaata
     1741 aaaaccgtgc gaaaaacggg acaggatgtg ggaatgtcgg accgcgcacg cgcactcaga
     1801 cattccccac ctggtcaggg ctgcgccccg acaccctcga caccgttccc gcaatacgat
     1861 agaacatcag ggcgcgtttc cgggtaaacg catgttaatg accgggaatt cattgattag
     1921 ggaccggaga cgcgctacaa aactcctgcg cgtttctggc atcaactgac catggggatg
     1981 gtttggcagt agcagcaagc gccagaaacg cgctcaaaac gtctctgacg gcctgaatcc
     2041 aacaaaacat acaaaaccat tgcccgatgc aaaaaagtgc gttagaaagc gtctgagcgc
     2101 gtttctggcg tcttttttgt tgctgtgtcg ctactcgttg tcagattcgc gctcaatcct
     2161 tcatcctgtc agcttcagga ttccgcttgt aacgtttttc cgtacgtccc cagtcaaacg
     2221 ccagccagaa ggcttgctcc tcgaactcgt ccggttctgc cggaatatct gccccaacca
     2281 aatcctcgtc tgtctccggt ttatcgactt tcagaacctc tttgagacga ccgccggttg
     2341 ccaccatccg aagcccgtag gtctgccgcg ccagctccag aaaccacgcc ggatcacgca
     2401 caacttccga agatttcacc gtgtatttca tcgtctcaat gacgcctgac tccagcgccg
     2461 cacgatgcat atcaggcagg gttacaccat ccggctgttt ccagccctgt ttcggtttta
     2521 ccacccgaat atccactccc gcctcataat cgacacgaag gcattcactc cagatctcca
     2581 cccagcgagc atgtttcaca taaccattac cggacaacat cgaagggggg accatcatca
     2641 gaacgtgaaa atgcggatgg caacatcctg gcactcgccc gtaagtaatt tccgtcgtac
     2701 gtaaccagcc aagaaccggc ctgaattcct tacgatcact caagcgtttc catgctgcat
     2761 tcatccgaat gagtgtttcg cgggtatttt ccacaggggg atttttgaca gtcagcgtca
     2821 tgaacaacca gcgggccgca ggacgttcaa ccaacagctt tggcaaggcc tcccagagac
     2881 gacgcatcca acggagagag cgacgacgac tacacaccgg acaaaacgga acatgacaaa
     2941 actccccctt ccgaagagcc agcttcattt cgccagtcag gcgggacacc ttcggcgcaa
     3001 acaacaaccg acgggaacaa tcaaacatcc gctctccacg agaggaaaac atcgaatcgc
     3061 tttcgtaaat ctgcgccacc ctgtcagcca tcacgcgatc gctgtcccat tttgtatccc
     3121 gtgtgctgta atcgcgcaac gagagtaccg gactatctgg atttttcatt tgcacccccc
     3181 aagaactggg cagatgcgga aattttgtgt tagcattttt ctgtcacctg tgactctgtt
     3241 gccgaacaac gaattccaaa ctggtgatcg tcccctggag tgtgaccgca ctcgcagggg
     3301 aaccccctca aaactgttca ttttttaaac aaacattttt atctcttttc cacccatttt
     3361 taccgctcaa agccgcgcca ccactggcct aagccaaaat aacacagacc gcagttgttg
     3421 cctatagtat atatacaagg atcgcgcgca cgatcgccca ccaaaaatgc gcgttttttg
     3481 agcgcttccg agcac
//
LOCUS       DAZGQL010000218         3355 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.220, whole genome shotgun sequence.
ACCESSION   DAZGQL010000218 DAZGQL010000000
VERSION     DAZGQL010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3355
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.220"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..587)
                     /gene="dsbA"
                     /locus_tag="ACHKIR_005100"
     CDS             complement(<1..587)
                     /gene="dsbA"
                     /locus_tag="ACHKIR_005100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HFO9992694.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQ"
     gene            complement(604..1590)
                     /gene="srkA"
                     /locus_tag="ACHKIR_005101"
     CDS             complement(604..1590)
                     /gene="srkA"
                     /locus_tag="ACHKIR_005101"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HFO9992695.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEDRRRFVVKFYRPERWTPDQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(1667..1936)
                     /locus_tag="ACHKIR_005102"
     CDS             complement(1667..1936)
                     /locus_tag="ACHKIR_005102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HFO9992696.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            2006..2590
                     /gene="mobA"
                     /locus_tag="ACHKIR_005103"
     CDS             2006..2590
                     /gene="mobA"
                     /locus_tag="ACHKIR_005103"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HFO9992697.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQASGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLNHQRKDAPVVWIHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDRKEAFINVNTPEELARWQEKR"
     gene            2572..3099
                     /gene="mobB"
                     /locus_tag="ACHKIR_005104"
     CDS             2572..3099
                     /gene="mobB"
                     /locus_tag="ACHKIR_005104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HFO9992698.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPVLCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLHFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALMDINDVE
                     GLADFVVEWMQKQDG"
     gene            complement(3202..3317)
                     /gene="rrf"
                     /locus_tag="ACHKIR_005105"
     rRNA            complement(3202..3317)
                     /gene="rrf"
                     /locus_tag="ACHKIR_005105"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          869 a          826 c          817 g          843 t
ORIGIN      
        1 tgctgaacaa aaacatccat attgctggta tccataccct gcggattcag ctgatattta
       61 ccgttaacaa acatcgccgg aacgccacgc aattgcacgt cagctgcagc tttttcctgc
      121 tgagcgacca gggatttcac cacgaagctg ttccacgctg cgtcgtactc ttcaccttta
      181 atacctgcgt tgataaatac atcgcggata tcagatgctg aacgaatggt ctgggttttc
      241 tgtacgcctt caaacagcgg aacagtgact ttgtcttcca cgcccagtgc catcgccact
      301 gcccatgcct gagtcagatc tttgcccagg tcacccccca tgaagttgac gtggtattta
      361 gtcatcttca cgccttccgg cagttttttc ttcacgttat cagaaatatg cagaacttct
      421 tcaaactgat agcagtgcgg gcagaagaaa gagaaaaact ccagcacttg cggcgcgcca
      481 gcaactggtt tttccagggt agtgtactgt ttaccatctt catactgcgc cgccgatgcg
      541 ctaaacgcta aaactaaacc agccagcgcc agccaaatct ttttcatgat ctactctctc
      601 cgattaatac ataggtgtta attgcaaagg gggttcttgt agaacttttg cctgttctat
      661 aaaagtcgcc gtctgtcgca gccagtaatc ttccccggtt aaccacggga aatttttcgg
      721 gaacgcggga tcagcccaac gccgcattag ccaggcaaga taataaacca aacgcatggc
      781 gcgtaaaggt tcaatcagtc cgatttcagc ggtgtcgaac tcgctaaatt cttcataagc
      841 ttcaataata gtttccagtt gcatccgctg ctcggcttta tcgccattga gcaacatcca
      901 caaatcctga atagccggac cattacgtgc atcatccaga tcaacaaaca ttggaccatc
      961 gcgccagaga atattcccgg cgtggcagtc tccatgtagc cgcagaacgg tgaaatcttc
     1021 ccgccagtgt gctgtaacgg cggcgatcag ctcatctgtc gctttcagaa atgccgcttt
     1081 caacccggaa ggtatcagtg tagcgtcctc aaacagcttg cgaggctcaa tgagatattc
     1141 attcagaccg atggtcgggc gatggataaa aagctgtttg cgccccgtct ggtgcatacg
     1201 ccctaaataa cgcccaaccg cttccatctg atcgatatta tcagcttcga actggcgacc
     1261 accgacgctt ggaaaaacag cgaaataaaa tccctgatga ttcaataaag tctgaccgtt
     1321 aaaggccaca ggtgctgcga ccggaacttc atcatttacc aactgcaacg caaattgatg
     1381 ttcttcgagg atttgatcgg gtgtccaacg ttcaggacga taaaatttga cgacaaaacg
     1441 tcgacgatct tcgtcctgaa attgatagac acggttttca tagctgttaa gcggggtaag
     1501 accggaatcc acccggatcc cttgctcaaa cagagcatcc atgatggtat ccgggtgtag
     1561 tgtctggaaa gtaaaagcgc tgttgttcat cccatcatcc ggaaaacatt acgaatgatg
     1621 aaggatatca tttcacgatg attttggtgg caccgcttac aagcttttac tctttaatta
     1681 cgccgcgagc gcgtaacagc gccgttttga agtcttcttc ataatctttc tgcaaacccg
     1741 ggatcaccgc atctttcgcg gaatcacgca ttttcaggtg atagatcaga atgtcatccg
     1801 tcaaatccgc cagttcgccg tcaaaacctg actctttcgc cagtttctgc aaaaattgca
     1861 gcaggttaag gtctggctct ttttgccagg ctggctggag gagttcaata acttcattca
     1921 gacgtttaca tttcatagta atgctcctta ctcttgagac agacacgtta gcagggtcaa
     1981 tcccacaata aaagaggcga tatcggtgaa tctgatgacg acgataacag gcgttgtgct
     2041 ggcaggcggt aaagccagac gaatgggcgg cgtagataaa ggattgcttg aattaaacgg
     2101 caaaccatta tggcaacatg tcgctgacgc gcttatgacg cagctctctc acgtcgtggt
     2161 taatgctaat cgtcatcagg aaatctatca ggcaagcggt ctgaaagtga ttgaagattc
     2221 actggcggat tacccaggcc ctctggcagg aatgctttca gtaatgcagc aggaagcggg
     2281 tgagtggttt ttgttttgtc cgtgcgatac gccttacatt ccccatgatt tagccgcccg
     2341 gcttaatcat cagcgcaaag atgcgcctgt cgtgtggatc cacgacggtg aacgcgatca
     2401 cccgactatt gctctggtaa accgcgcaat tgagccttta ttactggaat atctgcaagc
     2461 aggagaacgc cgggttatgg catttatgcg tctggctggc ggtcatgcgg ttgatttcag
     2521 cgatcgtaaa gaggcattta taaacgtgaa tacgccagag gagcttgccc gatggcagga
     2581 aaaacgatga taccgttact cgcctttgcc gcgtggagtg gtactggaaa aactacactg
     2641 ctgaaaaaac tgatcccggt attatgcgcc agagggatcc gtccgggact gattaagcat
     2701 acgcaccatg atatggatgt tgataaaccg ggcaaagata gctatgagtt gcgcaaggct
     2761 ggcgcggcac aaaccatcgt tgccagccag caacgatggg ccttgatgac ggaaacacca
     2821 gacgaagaag agctggatct acattttctc gcaagtcgaa tggatacctc aaagctggat
     2881 ttgattctgg tcgaagggtt taagcatgaa gagatcgcaa agattgtgct gtttcgcgat
     2941 ggagccggac atcgaccgga agaattagtg atagacagac atgttattgc tgtagccagt
     3001 gatgtgccgc ttaatcttga tgtcgcgtta atggatatta atgatgttga ggggctggct
     3061 gattttgtgg tggagtggat gcaaaagcag gacggataag caaaagtagg ctggataaga
     3121 cgcgccagcg tcgcatccgg tatttattct gctccagcgc aaaaaggcca tcctgacgga
     3181 tagccttctg cttaatttga tgcctggcag ttccctactc tcgcatgggg agaccccaca
     3241 ctaccatcgg cgctacggcg tttcacttct gagttcggca tggggtcagg tgggaccacc
     3301 gcgctaaggc cgccaggcaa attctgtttt atcaacacgt ctcgttatga catgt
//
LOCUS       DAZGQL010000219         3347 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.221, whole genome shotgun sequence.
ACCESSION   DAZGQL010000219 DAZGQL010000000
VERSION     DAZGQL010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3347
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.221"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..2493
                     /locus_tag="ACHKIR_005106"
     CDS             <1..2493
                     /locus_tag="ACHKIR_005106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFO9992699.1"
                     /translation="SVKVLDMNAIFQGGHGTAGDLYKTTFYGPTQYYLLKKPKFGSVL
                     MGALKNTSEWQFAGTDLNQAVDMAKNNKLTSSAQASYLYHGKLLGNMDIVIPELTGND
                     ILTLDGSVSISGDMSKQDGALIFQGHPVIHAGQTVSASQSDWENREFSLNNLNLNNAD
                     FSLSRNAFMNGNIRAVNQSTVIIGGDTVFTDKNDGTGNDVISVEGKSAAAGTSSYTGH
                     ITLEQKSALDIRDNFRGGVTSEDSHINVSSSSVLFSDASSFINSSLNIHKGGALTAQG
                     GLFTSGSIDIGDASLLLTGTPVNSDDAAFLPTINMADGGFKLMSDSSVLKARDQASVV
                     GDIISDKQATISFGTESGKEGILSEKASRGLAVGLLSGFNTAYRGAIHAPSASATVNN
                     TWWQLTGDSSLRSLKNTGSMTYFTGSAANKAFHTLTVDELTTNGTAYAMRTDLKNADK
                     LVVNQKLSGKDNILLVDFLNKPTGEKLDIELVSAPGNSSKDVFKGSEQAIGFSNVTPV
                     ITAIDAGDKTTWNLTGYRMAENPAATQSASGLASVGYKSFLSEVNNLNKRMGDLRDIN
                     GEAGAWARIMSGTGSAGGGFSDNHTHVQVGVDKKHELDGLDLFTGFTVTHTDSSASAD
                     AFKGKTKSVGAGLYASAMFDSGAYIDLIGKYVHHDNEYTATFAGLGTRDYSTHSWYAG
                     AEAGYRCHVTEDTWIEPQAELVYGAVSGKQFAWKDQGMHLSMKDRDYNPLIGRTGVDV
                     GKSFSGKDWKVTARAGLGYQFDLLANGETVLRDASGEKRIKGEKDSRMLMSVGLNAEI
                     RDNVRFGLEFEKSAFGKYNVDNAVNANFRYSF"
     gene            3026..3346
                     /locus_tag="ACHKIR_005107"
                     /pseudo
     CDS             3026..3346
                     /locus_tag="ACHKIR_005107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997724.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          931 a          768 c          842 g          806 t
ORIGIN      
        1 agcgtgaagg tgttagatat gaacgccatc tttcaggggg gacacgggac agccggagat
       61 ttgtataaaa caacttttta cggcccgaca cagtattatc tgctgaaaaa acctaaattt
      121 ggcagtgtgc tgatgggggc actgaaaaac accagcgaat ggcaatttgc aggaacagac
      181 ctgaatcagg ccgttgatat ggcaaagaat aataaactga catcaagtgc acaggcttca
      241 tatctttatc acggtaagct cctggggaat atggatattg tcattccgga gcttacaggc
      301 aatgatattt taacgcttga tggctctgtc agtatatcag gagatatgtc aaaacaggac
      361 ggtgctctta tcttccaggg acacccggtt attcatgcag ggcaaactgt ttctgcatcg
      421 cagagtgact gggagaacag ggagttctca ctcaacaatc tgaatcttaa taatgcggac
      481 ttcagtctgt cccgtaatgc atttatgaac gggaatatca gggccgttaa ccagagcact
      541 gttattatcg gcggagatac agtctttact gataaaaatg acggaacagg taatgatgtc
      601 atcagtgttg aagggaaatc tgctgccgca ggaacatcct cctatacagg gcatatcact
      661 ctggagcaaa aatcagcact ggatatccgc gataattttc gtggcggggt tacgtctgaa
      721 gacagtcata tcaatgtttc ttcatcttca gtcctgttct cagatgcatc gtcatttata
      781 aacagctccc tgaatattca taaaggaggt gcgctgaccg ctcagggagg gctgtttaca
      841 agtggaagca ttgatattgg tgacgcttcc cttctgctta ccggtacacc agtgaattca
      901 gatgatgctg cttttttacc gaccatcaat atggctgatg gcggatttaa actgatgtct
      961 gattcatcag tactgaaagc cagagaccag gcatctgttg ttggtgatat tatttctgat
     1021 aaacaggcca caatcagctt cggaactgaa tcaggtaaag agggcatatt gtctgagaag
     1081 gcatcccggg gactcgcggt aggattactg agtggtttta atacggcata ccgcggtgca
     1141 attcatgccc cgtcagcatc tgccactgtg aacaacacct ggtggcaatt gacaggagac
     1201 tcctcacttc gctcgttaaa aaataccgga agcatgacat attttacagg aagtgcagcg
     1261 aataaagcat tccatacact gacggttgat gagctgacga cgaatggcac tgcgtatgcc
     1321 atgcgtacgg acctgaaaaa tgcggataag ctggtagtaa accaaaagct gtcaggtaag
     1381 gacaatattc tgctggttga ttttctgaac aaacccaccg gagaaaaact ggatattgaa
     1441 ctggtgagtg caccggggaa cagcagtaag gatgttttta aaggaagtga acaggcaata
     1501 ggtttcagca atgtcacgcc tgttattaca gctatagacg ccggagataa aacaacatgg
     1561 aacctgaccg ggtacaggat ggcagaaaat cctgccgcaa cccaaagtgc ctcaggcctt
     1621 gcatctgtgg ggtacaaatc atttttgagt gaggtcaaca acctgaataa acgtatgggt
     1681 gacctgcgtg acatcaatgg tgaagctggc gcatgggcac gtatcatgag cggaaccggc
     1741 tctgccggtg gtggtttcag tgacaaccac acacatgttc aggtcggtgt cgacaaaaaa
     1801 catgagctgg acggactgga tttgtttacc ggcttcactg tcacacatac tgacagcagt
     1861 gcctctgctg atgctttcaa aggtaaaaca aaatctgtgg gggccggact ctatgcttcc
     1921 gccatgtttg attccggtgc ctatatcgac ctgattggta agtatgttca tcatgataat
     1981 gagtacaccg caacctttgc cggactcgga acccgtgatt acagtacgca ttcatggtat
     2041 gccggtgctg aagcaggcta ccgctgtcat gtcactgagg atacctggat tgagccacag
     2101 gcagaactgg tttacggtgc tgtatccggt aaacagtttg catggaagga ccaggggatg
     2161 catctgtcca tgaaggacag ggactacaat ccgctgattg gtcgtaccgg tgtggatgtg
     2221 ggtaaatcct tctcaggtaa ggactggaaa gtgacagccc gtgccggtct gggctaccag
     2281 ttcgacctgc tggctaacgg cgaaaccgta ttgcgggacg catcaggtga aaaacgtatc
     2341 aaaggtgaaa aagacagccg tatgctgatg tccgttggcc tgaatgcaga aatcagggac
     2401 aacgtccgct ttggactgga gtttgagaaa tccgcctttg gtaagtacaa cgttgataat
     2461 gctgtcaacg ctaacttccg ttactcgttc tgatactccg tattcaaaag ggcctgaggg
     2521 ccctttatgc tcaggacctg ccgcccgggt atacatttcc ggagtatcag tcgatttgtt
     2581 tacttaaccg ccatgaccaa agccggcagt aatgtcggct ttttacgtcc atatattcca
     2641 ctcatccgga actgtgacac agaacctgaa tacaggattg atcaccggtg cccacaaatg
     2701 gcacattttc agtaagattc tgaatgttac attccccctt catgtgtgac acagctgtca
     2761 cagagaacga aacggaaagc acatgaaaaa cataaaaact cacaccggac tccttatcca
     2821 caaagaacaa acacggcgcg ttcgtcttca tgaaacaccg gccgcctggt gccacacacg
     2881 ccgggaatgc tacagcaaaa cgaccggtcg tcgctgcggc agccctgaca gtctcagcag
     2941 gctgattctg agcagcattc agcccataga atgagataac ccgtatgaat gatgacagac
     3001 gtctacggta cccgatgtat ggaacatttg ctttctggct aacggcatgc ccgacacatg
     3061 gttttacacc gatgacgcct ccgtcacaaa agagtggggg cgggaatatt gtgacaatgt
     3121 ggaagtgttc cctctgtgct atatgcatga cggtcccgtt ttccgggggc gctatattga
     3181 tgagctcagc gccgcacgga acagaatgct ccatatgcac cacatactgc cggaatgaac
     3241 ccggtacaga gaaaattttc atgacgcaga atgtatccgt gaaattcacg aagcgcccct
     3301 gcaggctccc ggcattctgc aaaaatacaa acgcacaaaa tcataag
//
LOCUS       DAZGQL010000220         3331 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.222, whole genome shotgun sequence.
ACCESSION   DAZGQL010000220 DAZGQL010000000
VERSION     DAZGQL010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3331
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.222"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            103..978
                     /locus_tag="ACHKIR_005108"
     CDS             103..978
                     /locus_tag="ACHKIR_005108"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000629094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009384 - N-acylmannosamine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HFO9992700.1"
                     /translation="MITLAVDIGGTKISAALISDDGSFLLKKQISTPHERCPDEMTGA
                     LRLLVSEMKGTAERFAVASTGIINNGVLTALNPDNLGGLKEYPLKNIMEDITGLNGSV
                     INDAQAAAWAEYTVLPKEICDMVFITVSTGVGGGIVVNRKLLTGVSGLAGHVGHILSG
                     VTDTECGCGRRGCVEAVSSGRAIMGAAKNELAGYSTKYIFELARQGYKEAEFLTERSA
                     STIAELIVSLKLLLDCQVVVVGGSVGLADGYIQKVSKHLSIYSEICNVMLFPAYFRSD
                     SGLIGATLWDRDCIT"
     gene            1027..2499
                     /locus_tag="ACHKIR_005109"
     CDS             1027..2499
                     /locus_tag="ACHKIR_005109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9992701.1"
                     /translation="MDSCIQKKQVWYKHLSPRQWKIFGAAWTGYLLDGFDFVLISLVL
                     TEVQHEFGLTTIEAASLISAAFISRWFGGLAIGALSDKMGRRMAMVLSIVLFSLGTLA
                     CGLAPGYAVMFIARIVIGLGMAGEYGSSVTYVIESWPVHLRNKASGFLISGFSIGGGL
                     AAQVYSIVVPLWGWRSLFFVGMLPILFAFYLRKNLPESDDWQKRQQENKPVRTMVDIL
                     YREKNKYINILLSCIAFACLYVCFSGVTANAALITVMALCCAAVFISFIYQGMGKRWP
                     TGIMLMLVVMFCFLYGWPLQAFLPTWLKVDMQYSPETVALIFMLAGFGSAAGSCIGGF
                     MGDWLGTRKAYVISLLIGQLVIIPVFLVDRDYVWLLGLLIFTQQVFGQGIGALVPKII
                     SGYFNVEQRAAGLGFIYNVGSLGGACAPILGAVVASHTSLGTAMCSLAFILTFVVLVL
                     IGFDMPSRVQRWIHPEAALEYDTVDGKPFYGARKKNVAGE"
     gene            2503..>3331
                     /locus_tag="ACHKIR_005110"
     CDS             2503..>3331
                     /locus_tag="ACHKIR_005110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000948499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO9992702.1"
                     /translation="MLSGNKKMIHYRENIYSLDNYERYITDNSLFENTDDGIIYTGIV
                     PLITVVEPVVSNQHSVLIIPGGGYRRIVYEREGLDVGRWIAGQGYTAFILSYRMPSVN
                     GYYSFADAKNALNFISYWAGMSERTFKSVGCMGFSAGGHLAGYITHLHYEEETSVRPD
                     WMALIYPVISLDRSIAHQGCRKRLLDAGESEYSASLENSVTEYMPPVFIVHATDDKDV
                     PVIHSLRYFNSIRDKGHHAEAHFFDKGGHGFGVSGKNGYPIEKWQSLLLEWLAEHKFH
                     "
BASE COUNT          891 a          530 c          832 g         1078 t
ORIGIN      
        1 tgtattatca aaacttgcag agcgactgct cgaagaacat gacagaaata aaaaaatgaa
       61 aataatctga ataattgcaa gattgttata taggtaaaat gaatgataac actggctgtt
      121 gatataggag gaacgaaaat ttcagcggcg ttgatatctg atgacggttc atttttgctg
      181 aaaaaacaga tatcaacacc tcatgaaaga tgtcctgacg aaatgacagg ggctttacgt
      241 ctactggttt ctgaaatgaa gggaacagcg gagcgatttg cagtagcatc aacagggatt
      301 attaataatg gagtattgac agctttgaat ccagacaatc ttggagggct gaaagagtat
      361 cctctgaaaa atataatgga agatataact ggtttaaatg gaagtgtgat aaatgatgcc
      421 caggcagccg catgggcaga atatacagtt ttgccaaaag aaatttgtga tatggttttt
      481 ataaccgttt ctacgggggt tggaggggga attgtagtta acagaaaatt attaacaggt
      541 gtttctggat tagccggaca tgttgggcat atattgtccg gagtaacgga tacagaatgt
      601 ggatgtggtc gtcggggatg cgttgaagcg gtttcatctg gtcgggccat catgggggca
      661 gcaaaaaatg aacttgcagg atatagcacc aaatatattt ttgaattagc gagacaggga
      721 tataaggaag ccgaattttt gacagagcgt tctgccagta ctattgctga attaatagta
      781 agtttaaaat tactgcttga ttgtcaggtt gttgttgtcg gaggaagtgt tggacttgct
      841 gatgggtata ttcagaaagt gtcgaaacat ctttccatat attctgaaat ttgtaatgta
      901 atgctgtttc cggcatattt cagaagtgac tctggtctta ttggtgcaac tctttgggac
      961 cgggattgta ttacttaaag taatactcca gtgtattata aatccgctat tattgtgagg
     1021 tttaatatgg actcctgtat tcagaaaaag caggtttggt ataagcatct ttcacccaga
     1081 cagtggaaga tattcggtgc ggcatggaca ggttatttac ttgatggttt tgatttcgtt
     1141 ctgatatctc tggttcttac agaggttcag catgaattcg gcttgacgac aatagaagcg
     1201 gcaagcctta tttccgctgc ttttatttcc cgatggtttg gggggcttgc tattggcgct
     1261 ttatcggaca agatggggcg caggatggca atggttttaa gtatagtgct attctctctc
     1321 ggaacccttg cctgtggtct ggctcctgga tatgcagtga tgtttattgc tcgcattgtt
     1381 attgggcttg gtatggcagg tgaatatggc tcaagtgtaa catatgttat cgaaagctgg
     1441 cctgttcatc tcagaaacaa ggcaagtggt tttttaattt ctggattttc tattggggga
     1501 ggtttagctg cacaggttta tagcattgta gtaccactat ggggctggcg ttctcttttc
     1561 tttgtcggca tgcttccaat attatttgct ttttatcttc gcaagaatct tccggagtct
     1621 gatgactggc aaaaaaggca acaagagaat aaacccgtca gaacaatggt ggatattctg
     1681 tacagagaaa agaataaata cataaatatt cttctgtctt gtattgcatt tgcatgtcta
     1741 tatgtttgtt tttcaggcgt cactgcaaac gctgctttaa tcacagttat ggcattgtgc
     1801 tgtgcggctg tttttatcag ttttatatat cagggtatgg ggaaaagatg gccaacaggc
     1861 attatgttga tgcttgtggt tatgttctgt ttcctctacg gatggccact gcaggctttt
     1921 cttcctacat ggcttaaggt tgatatgcag tattcaccag aaactgttgc tctcatattt
     1981 atgctggcag gttttggctc tgcagctgga agttgtatag gtggttttat gggggactgg
     2041 cttggaaccc gaaaagcata tgttatatca cttcttatcg gtcagcttgt tattatcccc
     2101 gtttttcttg tggaccggga ctatgtatgg ttacttggcc ttctgatctt tactcagcag
     2161 gtttttggtc aggggattgg tgctctggtg ccgaaaataa tcagtggtta ttttaatgtg
     2221 gagcagcgtg cggcaggact ggggtttatt tataatgtag gttcacttgg tggagcatgt
     2281 gcgcctattc ttggtgcagt tgttgcgtct catacaagtc ttggtacagc aatgtgttct
     2341 cttgctttta tcttaacatt tgttgttctt gtccttattg gttttgatat gccatcacgt
     2401 gtacagagat ggatccatcc agaagctgcg cttgaatatg atactgtcga tggaaaacct
     2461 ttttatggag caagaaaaaa gaatgttgca ggagaataaa ggatgctatc agggaataaa
     2521 aaaatgattc attacaggga gaatatatat tctcttgata attatgagcg ttacataact
     2581 gacaattcgc tatttgagaa tactgacgat ggcataatat atacaggtat tgtgcccctt
     2641 attacagttg ttgagcctgt tgtatctaat caacatagtg tgctgattat acctggaggt
     2701 ggatacagga ggattgttta tgaaagagag ggacttgatg taggcagatg gatagcaggt
     2761 cagggatata cggcatttat tctttcttac agaatgccct ccgttaatgg ttattattcc
     2821 tttgcggatg cgaagaatgc cctgaacttt atttcttact gggccggaat gtcggagaga
     2881 acatttaaga gtgttggatg tatggggttt tcggcaggtg gtcatcttgc tggatatatt
     2941 actcatctcc attatgagga agagacttct gtgcgtcctg actggatggc tctgatatat
     3001 ccagttatat ctcttgacag gagtattgct catcagggat gccggaaacg tcttctggat
     3061 gcaggtgaaa gtgaatattc agccagtctt gaaaatagtg ttacagagta tatgccacca
     3121 gtctttattg tgcatgcaac agatgataag gatgttccag ttattcactc tctgagatat
     3181 tttaactcta tccgggataa gggacatcat gcagaagctc atttttttga taagggaggg
     3241 catggatttg gtgtatccgg aaaaaatgga tatccgatag aaaaatggca aagcctgctt
     3301 ctggaatggc ttgcggaaca taagtttcat t
//
LOCUS       DAZGQL010000221         3320 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.223, whole genome shotgun sequence.
ACCESSION   DAZGQL010000221 DAZGQL010000000
VERSION     DAZGQL010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3320
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.223"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..302
                     /locus_tag="ACHKIR_005111"
     CDS             <1..302
                     /locus_tag="ACHKIR_005111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001599750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992703.1"
                     /translation="SKNSKNTEFNTLKNRKEFLSSVINVNETRLQASAPAMPAIEALN
                     AFIDKGDYLKSLYVNKTRTAAEQKRLNEILRAQVISGLKASEGLAKLLIASGIML"
     gene            313..621
                     /locus_tag="ACHKIR_005112"
     CDS             313..621
                     /locus_tag="ACHKIR_005112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001590091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992704.1"
                     /translation="MNDVQNRRILALLDMLYNTLGNKTTCNVCHKYFILILTVMNNLT
                     INYGTETSPELSGISYEDANALITELILFIEDVDAENHEAYQGHLNEITRILNFAIAA
                     "
     gene            complement(673..1263)
                     /gene="cobO"
                     /locus_tag="ACHKIR_005113"
     CDS             complement(673..1263)
                     /gene="cobO"
                     /locus_tag="ACHKIR_005113"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HFO9992705.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGIVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADPSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPVKHAFDAGVKAQIGIDY"
     gene            complement(1260..2018)
                     /locus_tag="ACHKIR_005114"
     CDS             complement(1260..2018)
                     /locus_tag="ACHKIR_005114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HFO9992706.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSEDCQQLAQRIAVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            2238..3287
                     /gene="sohB"
                     /locus_tag="ACHKIR_005115"
     CDS             2238..3287
                     /gene="sohB"
                     /locus_tag="ACHKIR_005115"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HFO9992707.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
BASE COUNT          875 a          761 c          848 g          836 t
ORIGIN      
        1 tatctaaaaa ctctaagaat acagagttta acactctgaa aaacaggaag gagtttctaa
       61 gttcagtaat caatgtgaat gaaaccagac tacaggcatc ggcacctgcc atgcctgcga
      121 ttgaggcttt gaatgccttt atagataagg gtgattatct gaaaagctta tacgttaata
      181 aaacacgcac ggcagctgaa caaaaaagat taaatgaaat attacgcgca caggtcatta
      241 gtgggttaaa ggcgtctgag gggctggcaa aattacttat tgccagcgga ataatgttat
      301 aggaggcttt ttatgaacga tgtgcaaaac cgtagaatac tggcattact tgatatgctg
      361 tataatactt tgggtaataa aaccacatgt aatgtttgtc ataaatattt tatccttatc
      421 ctcacggtga tgaacaatct gacgattaac tacggtactg aaacttcgcc agagttaagc
      481 ggaatcagtt atgaagatgc caatgcgtta attactgaac tgatattatt tattgaagat
      541 gttgacgctg agaatcatga agcatatcag ggacatttga atgagattac acgtattctg
      601 aatttcgcta ttgccgcttg aatataatca tgattaaagc acctcaagag gcttcttttg
      661 aggtgctttc acttagtagt caatccctat ctgcgctttt acaccggcat caaacgcatg
      721 tttcacgggg cgtaattcac tcaccgtatc cgccagttca agaatatccc gatgacagcc
      781 tcgacccgtg ataatcaccg tctgttgatg tggacgttca tttaacgcct gcaccacttc
      841 ttccagtggc aaatagtcat acgccaccat atacgtcagt tcatcgagca aaaccatatc
      901 cagtgaagga tcggccagca ttcgtttggc atgttgccag acttcacggc aggccgcggt
      961 atcagattcg cgattttgtg tatcccaggt aaagcccgtt gccatcacct gaaactcaac
     1021 gccatgtggc tccagtagat tgcgctcgcc attaggccag gtgcctttaa taaactgcac
     1081 gatacccact tttttgccat ggcctactgc acgagttgcc gtaccaaatg ccgcggtggt
     1141 tttgcctttt ccattgccgg taaagacgat gataataccg cgctcatcct gggcctgggc
     1201 cacacgagca tcgacttttt ctttcactcg ctgctgacgc tgttggtagc gttcatcact
     1261 cattgggaaa ttcctggttt acggcccggt tgggcgtcaa aggtcatgcc ggttttacgg
     1321 cggctgtcat cgcccatcag ccagaggtag agcggcatga tatcagcggg tgttttaagt
     1381 ttctgtggat cttcggtcgg gaaggcgctg gcacgcattg cggtgcgcgt accgcctggg
     1441 ttaatgcagt tgacgcgcag gcgctgctga tattcatcgg ccagtacctg catcatccct
     1501 tcggtggcaa atttcgacgc tgcatatgcg ccccagttgg ctcgtccctg acgtccaacg
     1561 cttgatgaag taaagaccag tgaaccggcg tccgatttga gtaataaagg aagcagtgcc
     1621 tgggtgagca taaaggtggc attaacgttg acctgcatga cgtcctgcca gacctgcgga
     1681 ttttgttcgc tcattgggca aacatcgccg agcaatccgg cattatgcaa aacaccatcc
     1741 agacgcggat aattaacggc aatgcgctgt gccagttgtt ggcaatcttc ggacgtgcag
     1801 gtcagcaaat cgagaataaa ccactgtggc tgacgtccag tttcttcgtt tatgtggctg
     1861 gctacctgac gtaatttttc ttcattacgg cccaacagaa tcactgtcgc accatagcgt
     1921 gcatacgtca tcgcggcttc acggccaata ccatcgctgg ctcccgtcac caggataatg
     1981 cgatcattga gtaaatcttg ttttggctgg taatgcatgg ctactcctca acgacgttgt
     2041 ctgtcgcgct gccagtgtac tttatgactt tggggcttta tgcctgaaac agaagggcat
     2101 ttcaatcagc ctggggcgat aacaccgcaa aatggatact ttgtcatact ttcgctgcaa
     2161 taacatctct gcgagacggc ttaacatgcc tgttgtaaac tgtgagccaa agcgttgttt
     2221 aaccaaggtg gggactcgtg gaattgttgt ctgaatatgg tttgtttttg gcgaaaatcg
     2281 ttaccgttgt gctagcgatt gcggcgattg ccgccattat tgtcaatgtt gctcaacgta
     2341 ataaacgcca gcgtggcgag ttacgggtca acaatctcag cgaacagtat aaggagatga
     2401 aagaagaact ggccgcggcg ctgatggact cacatcagca aaaacagtgg cacaaagcgc
     2461 agaagaaaaa gcacaagcaa gaagcgaaag cagcaaaagc gaaagccaaa ctgggcgagg
     2521 tggcaactga cagtaaacct cgcgtctggg tgctggattt taaaggcagc atggacgccc
     2581 atgaagtgaa ctcgctacgt gaagagataa cggcggtact cgcagcattc aaaccgcagg
     2641 atcaggttgt gctccgtctg gaaagccctg gtggcatggt gcatggttac gggttggcgg
     2701 cttcgcagct gcagcgtctg cgtgataaaa acattccttt aactgtaacg gtagacaaag
     2761 tcgctgccag cggcggttac atgatggcct gtgtggcgga caaaattgtt tccgcaccgt
     2821 ttgctattgt gggttccatt ggcgtggtgg cgcaaatgcc caactttaac cgcttcctga
     2881 aaagcaaaga tattgatatc gaactgcaca ccgccgggca gtataagcgc acgctgacct
     2941 tgctgggtga aaacaccgaa gaagggcggg agaaattccg cgaagagctg aacgaaacgc
     3001 atcagttatt taaagatttt gtgaagcgta tgcgtccgtc tctggatatt gaacaggtgg
     3061 caacgggtga acactggtac ggacaacagg cggtagagaa aggcctggtt gatgaaatca
     3121 acaccagtga tgaagttatt cttagcctga tggaaggccg tgaagtggta aatgtacgct
     3181 atatgcagcg taaacgactc attgaccgat tcaccggcag cgcggcagag agcgccgatc
     3241 gattgttgct acgctggtgg cagcggggtc aaaagccatt gatgtaaaag acaaacgcga
     3301 gggtaagacc tcgcgttttg
//
LOCUS       DAZGQL010000222         3253 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.224, whole genome shotgun sequence.
ACCESSION   DAZGQL010000222 DAZGQL010000000
VERSION     DAZGQL010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3253
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.224"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            127..1338
                     /gene="bluF"
                     /locus_tag="ACHKIR_005116"
     CDS             127..1338
                     /gene="bluF"
                     /locus_tag="ACHKIR_005116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
                     /protein_id="HFO9992708.1"
                     /translation="MLTTLIYRSHIRDDEPVKKIEEMVSIANRRNMQSDVTGILLFNG
                     SHFFQLLEGPEEQVKMIYRAICQDPRHYNIVELMCDYAPARRFGKAGMELFDLRLHER
                     DDVLQAVFDKGTSKFQLTYDDRALQFFRTFVLATEQATYFEIPAEDSWLFIADGSDKE
                     LDSCTLSPNINDHFAFHPIVDPLSRRIIAFEAIVQKNEDSPSAIAVGQRKEGEIYTAD
                     LKSKALAFAMAHALDLGDKMISINLLPMTLVNEPDAVSFLLDEIKANALVPEQIIVEF
                     TESEVISRFDEFAEAVKSLKAAGISVAIDHFGAGFAGLLLLSRFQPDRIKISQELITN
                     VHKSGPRQAIIQAIIKCCTSLEIQVSAMGVTTPEEWMWLESAGIEMFQGDLFAKAKLN
                     GIPSVAWPEKK"
     gene            1553..2284
                     /gene="bluR"
                     /locus_tag="ACHKIR_005117"
     CDS             1553..2284
                     /gene="bluR"
                     /locus_tag="ACHKIR_005117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HFO9992709.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWSRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAAASLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPRPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
     gene            2505..2909
                     /locus_tag="ACHKIR_005118"
     CDS             2505..2909
                     /locus_tag="ACHKIR_005118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HFO9992710.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRVAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNLLKVSTKKNPAKIKEAALLHISGKSTFREYCSTLADAGVFR
                     WVTDVNHNKRSYYAIDNTLLYIEDVENNKPLI"
     gene            2962..3072
                     /locus_tag="ACHKIR_005119"
     CDS             2962..3072
                     /locus_tag="ACHKIR_005119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992711.1"
                     /translation="MEIKVQRLSLWMINTVFLLPPINNHQANTINLIFEM"
BASE COUNT          963 a          677 c          679 g          934 t
ORIGIN      
        1 atcttcaact ttttccattt tttatcaatg taaagaaaca gttatgtttt ggtattaaaa
       61 gaaagacacc ggaagatatg tctgttaccg tcttactctc gcctctccca ttaccctgga
      121 cagttaatgc ttaccaccct tatttatcgt agccatatac gtgacgacga acctgtcaaa
      181 aaaatcgaag aaatggtttc gatagcaaat cgcaggaaca tgcagtctga cgtaacaggg
      241 atcttactgt ttaatggttc tcattttttc cagcttctgg aaggtccgga agaacaggtt
      301 aaaatgatat atcgggctat atgccaggat ccacggcact ataatattgt tgagctgatg
      361 tgcgattacg cgcctgctcg ccgttttggc aaagcgggaa tggaattatt tgatttgcgc
      421 ctgcacgagc gagatgacgt tttacaggcc gtattcgaca aaggcacatc aaaatttcag
      481 ctaacttatg atgacagagc gctacaattt tttcgtactt ttgtccttgc aaccgaacaa
      541 gcaacctatt tcgagatccc tgccgaagac tcctggcttt ttatcgctga cggatctgat
      601 aaagaacttg attcctgtac cctttcacca aatataaacg accactttgc ctttcatcct
      661 attgtcgatc ccttatcgcg gcggataatc gcttttgaag ccattgtgca aaaaaatgaa
      721 gacagcccgt cagctatagc ggttgggcag cgtaaagaag gggaaatcta tacagcggat
      781 ctcaaaagta aggcgcttgc attcgcgatg gcacacgcac ttgacctcgg tgataaaatg
      841 atttcaatca atctattacc tatgaccctg gttaacgaac ctgacgcagt ctctttttta
      901 cttgatgaaa taaaggccaa tgctctggtg cctgaacaaa tcatcgttga atttactgaa
      961 agtgaagtca tatctcggtt tgatgagttt gccgaagcgg ttaaatcgct aaaggctgct
     1021 ggtatcagtg tagcaattga tcattttggc gcaggttttg ccggtttgtt actcctgtca
     1081 cgcttccagc ctgacagaat taaaatcagt caggaattga ttaccaatgt ccataaaagt
     1141 gggccacggc aggcaattat tcaggcgatc ataaaatgct gtacatcact tgaaattcaa
     1201 gtctctgcta tgggtgtgac aacaccagaa gagtggatgt ggcttgaatc tgcaggaatt
     1261 gagatgtttc agggagatct gtttgcgaaa gctaaattga atggtatccc ttcagttgcg
     1321 tggccagaga aaaaataatt ttcagcacat tatttcacat gatttcacat gatttccgta
     1381 aatcatcgtg aaaatgaata cgttatgttt aaatttaatc cacattaaaa ataatgtttc
     1441 attcaattta gctgaaatct tatttatact ttgtacaatt ttagcgtata ttgtacaaag
     1501 tattattggg tcgtgtacag gcgacggaga tttgtgaccg caaggaggaa ttgtggctta
     1561 ttacagcatt ggtgatgttg ctgaacgttg cgggattaat cctgtcactc tccgggcctg
     1621 gcaacgccgc tacggtttgt taaaaccaca gcgcagtgaa ggcggacacc gactctttga
     1681 tgaagaagac atacaacgca tcgaagagat caagcgttgg ataagtaatg gcgttcctgt
     1741 aggcaaagtt aaagcattac tggaaaccac cagccaggat acggaagatg actggagtcg
     1801 cctgcaagaa gagatgatgt caattcttcg tatggctaat cctgccaaac tacgcgcgag
     1861 aattatttca ctgggtcgag agtacccagt agatcaattg attaatcatg tttaccttcc
     1921 tgttcgccag cgtctcgtgc ttgatcacaa cacctcccgc attatgagca gtatgtttga
     1981 cggcgcatta attgaatacg cagcagcctc acttttcgaa atgcgccgta agcccggtaa
     2041 agaagccatt ctgatggcgt ggaatgttga agagagagca cgattgtggc tggaagcatg
     2101 gcgtttatca ttgtcaggat ggcacatttc tgttcttgct gatcccattg aatcgccgcg
     2161 cccggaactg ttcccgacgc aaacattgat tgtctggaca ggcatggcac caacgagaag
     2221 gcaaaacgaa cttttacaac actggggtga gcaaggttat aaagtcatct ttcatgcccc
     2281 ctaatttcac ctttatcccg ccccttagac agaatcttat aatctgaacc cacaacagct
     2341 tttgtaccgg agctggtccg ttgcaaaatg ctcaccggga gaattcaggt aatccccctt
     2401 tcacactttg tacatatcac agccaaagga cagaccagat tgaagtggta aaaccgatcg
     2461 tgcacactac gcagtctcac aactaacaag aggttattat cctgatggat caggtcgttg
     2521 tttttcaaaa gatgtttgag caagtgagaa aagagcaaaa cttctcctgg ttttattcag
     2581 aattaaaaca tcaccgcgtt gcacattaca tttattatct ggctacggat aacatcagaa
     2641 ttattactca cgatgacacg gttttgttat taagaggaac caggaacctg ttaaaagtta
     2701 gtacgaaaaa gaaccctgct aaaataaaag aggccgcatt gcttcatatt tccggaaaat
     2761 ctacatttcg ggagtactgt tcaacactgg cagacgcggg cgttttccgg tgggttactg
     2821 atgttaatca taacaaacgc agttactacg ccattgataa tacgctttta tatattgaag
     2881 atgtagaaaa taataaacca ttaatctagc taaagttgga tgcttaaaaa atgcttcata
     2941 attcagtaag acattagcat aatggaaata aaagtgcaga gactatccct atggatgata
     3001 aatactgtct ttttattgcc acccataaat aatcaccagg ccaatactat caacttgata
     3061 tttgaaatgt gatcacttga ctttctatac gttattttat aacggttaac atatttataa
     3121 aaccaacggg cgtgccatac gcccgtttca atacttaacg cacatgtgtt ttggtttagt
     3181 catcatccga ttatatgtat tttagtcagg aacgggttta accattccta tataactcaa
     3241 aaattgaaac ctt
//
LOCUS       DAZGQL010000223         3231 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.225, whole genome shotgun sequence.
ACCESSION   DAZGQL010000223 DAZGQL010000000
VERSION     DAZGQL010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3231
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.225"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            168..473
                     /locus_tag="ACHKIR_005120"
     CDS             168..473
                     /locus_tag="ACHKIR_005120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001604127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992712.1"
                     /translation="MNPLFNDIQMRLFYLNHAPYSWHWNVRFRPQEAVYIGNDACHIT
                     ITCNQSGFHLTRDGQRLFTERYIRTLSELLAVLKRRWDVTPAIIRAVEYLSRVPVSH"
     gene            528..1892
                     /locus_tag="ACHKIR_005121"
     CDS             528..1892
                     /locus_tag="ACHKIR_005121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
                     /protein_id="HFO9992713.1"
                     /translation="MTSEVKLKTGGDPRSLPDYAALRDEISKLTHPARPDVDWRYVET
                     LCLRLYEHNGVELQTVSWYTIARMHTAGLSGLNEGLALIVALTRHHWSVMWPLNTHAR
                     LEIITGLFSRLQKTLRAMPPDERDNLPLLYQTETCLKTLSDILARHELKQSSKVALPE
                     AMVKGYITRLENQPVQGEVSSPVTLPAQALRSDAPDVQEHQPLPYSRLVYVVSETKTE
                     PTLFLQKSPLTFLKTFVTGVCTALLAVCVAIPGWQFLTQPSPAEQQLRALLAPLPDVL
                     SADQMAQLSRTPSLLNHTSEWITLSGQQITRLTELPPAYNLQRSAQLLQQLRGLFPDN
                     PRVQEMVDNWHKSVRSRALPEEAMTGWNEGMARLQQLAERLNRLDEQRGKYMTVSELK
                     TEVFGIMQAFNRHIPAEEQLRRYDEARNQNGSEQQQKQAEMALNQLINRYQMENAGKP
                     KSQP"
     gene            1908..2540
                     /locus_tag="ACHKIR_005122"
     CDS             1908..2540
                     /locus_tag="ACHKIR_005122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SANT/Myb-like DNA-binding domain-containing
                     protein"
                     /protein_id="HFO9992714.1"
                     /translation="MEMENTPGRARTPWTIEDVRYLETHYGVKKTREIAAYLNRSATA
                     VRAMAYLVGITTPVAADWSETEIEIIRRDYPAGVALADICARLNGRAPKSVCWMARRF
                     GLKRLGYWSAEEDEILRQYYPSEGVKVSRRLSGRTENAVKLRARELGVKFTGNGRLRL
                     WTAEEWNVLSGNMASGLSALMKLLPGRSRSSVQGAMRRLQKMSAQKSVRQ"
     gene            2732..3231
                     /locus_tag="ACHKIR_005123"
                     /pseudo
     CDS             2732..3231
                     /locus_tag="ACHKIR_005123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000150682.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          910 a          718 c          844 g          759 t
ORIGIN      
        1 taaagatgca ggactggttc gttacggaac aacttttatg ccggagggca gggtgttact
       61 gcgatatctg aagcaacagc agtttgtgga taatgtgatg actttcaggg ggtgatttat
      121 ggttcccctt atccgtgtgc ttaacagaca taaacgaggg ctgaatgatg aacccgttat
      181 ttaatgacat ccagatgcgc cttttttatc tgaatcatgc tccgtattcc tggcactgga
      241 atgtcagatt caggccacag gaagccgtat atattggtaa tgatgcatgc catattacca
      301 ttacctgtaa tcagtccggt tttcatttaa ccagggacgg gcagcgactg ttcactgaac
      361 gttatatcag aacactcagt gagttattag cggttctgaa gcggcggtgg gatgtgacac
      421 cggcaatcat ccgtgcggtg gaataccttt cccgggtacc ggtatcacac tgatgtcctt
      481 tccgtaactg ttaatcatga tttaatacag caacaccgaa tctgccgatg acttctgaag
      541 tgaaactgaa aaccggtggt gacccccggt cattaccgga ttatgctgcc ctgcgtgatg
      601 aaatcagcaa actgacccac ccggcacgtc cggatgtgga ctggcgttac gtggaaacgc
      661 tctgcctgcg tctgtatgaa cacaatggcg tggaattaca gacggtatcc tggtacacca
      721 ttgcccggat gcatacagca gggttaagtg gtctgaatga agggctggct ctgattgtgg
      781 cgctgacccg gcatcactgg tcagtgatgt ggccgctgaa tacccacgcc cggctggaaa
      841 ttattaccgg tctgttcagt cgtctgcaaa aaacgctccg ggccatgcca ccggatgagc
      901 gtgacaacct gcccctgctg tatcagacag aaacatgcct gaaaacgctg agtgacatac
      961 tggcccggca tgaacttaaa cagagcagta aagtggcttt accggaagcc atggtgaagg
     1021 gatatatcac ccgtctggag aatcagcctg tgcagggtga agtatcgtcg ccggtaaccc
     1081 ttccggctca ggcactccgg tctgatgctc cggatgtaca ggaacatcag cccctgccgt
     1141 actcccgtct ggtgtatgtt gtttctgaaa caaaaacaga gcccacactc ttccttcaaa
     1201 aatccccgct cacgtttttg aaaacgtttg ttacaggtgt ctgtacggca ttgctggcgg
     1261 tctgcgtggc cattccggga tggcagtttc tgacacagcc atcaccggcg gagcagcaac
     1321 tcagggcgct gctggcacca ctaccggatg tgctttcagc agaccagatg gcgcaactca
     1381 gcagaacacc ctcactgcta aatcatacgt cagagtggat aaccctttcc ggtcagcaga
     1441 taacgcgact gacagaacta ccgccggcat acaacctgca acgcagtgcg cagctgctgc
     1501 agcaactgag gggacttttc cccgacaatc cccgtgttca ggaaatggtg gataactggc
     1561 ataagagcgt aaggagccgc gcgctcccgg aagaggcgat gacgggctgg aacgaaggca
     1621 tggcccgttt acagcaactg gctgagcgcc tgaaccgtct ggatgaacag cggggaaaat
     1681 acatgacggt cagtgaactg aaaacggagg tgtttggcat catgcaggct tttaaccggc
     1741 acataccggc agaagagcag ttacgtcgtt atgatgaagc ccgtaaccag aatggcagtg
     1801 aacagcagca aaaacaggct gaaatggcgc tgaatcagtt gattaatcgt tatcagatgg
     1861 agaacgcagg caaaccgaag agccagccat aatggctgag ataaaacatg gaaatggaaa
     1921 atacccccgg aagagcgcgc acaccatgga caatcgaaga tgttcgctac cttgaaacgc
     1981 attatggcgt gaagaaaacc cgcgaaatag cggcatacct gaacagaagt gcgacagcag
     2041 ttcgtgccat ggcgtatctg gtggggataa caacaccagt ggctgcggac tggtcagaga
     2101 cagagattga gattattcgc agggactatc cggcaggcgt tgcgcttgct gatatttgtg
     2161 cccgtctgaa cggacgagcg ccgaaatccg tgtgctggat ggcccgcagg tttggtctga
     2221 aacgcctggg ttactggagt gcagaagaag atgaaatcct gcggcaatat tatccttcag
     2281 aaggtgtgaa agtgagccga cgcctttccg gacgtacaga aaatgcagtg aaactgagag
     2341 cgcgggagct gggtgtaaag ttcacgggaa acgggcgatt acgtctctgg acggcagagg
     2401 agtggaatgt gttgtccgga aatatggcat cggggctatc ggcactgatg aagttactgc
     2461 cggggcgttc ccgtagttca gttcaggggg ctatgaggcg tttacagaaa atgagtgcgc
     2521 agaaatctgt caggcagtag ccaaaatatt cctgcatcag gagaacggca gtggcaataa
     2581 taagcagcaa actgatacat aatgcagctt ctgtaaacgg taatgatcaa gtagggatat
     2641 ttttatgttg actggttgtg aaaaaatgat caaaatttaa attaaccttt gcatgagatt
     2701 agaatctatt aattttatca aaggagattg tgtgacaata gtaagagatt ctgttagtgg
     2761 tgaaattgtt catagtcatg atcttcggga aatggatgtt ctttatatac aaaaaaccac
     2821 ttttgaatgt ccgtatgaca aatgtaggat acctgctact ccatgtagct tcaaagaaat
     2881 aaatttaaat caatcatact ttcgatataa agagcctcgt aggccgggat gtaagattca
     2941 tgatcctaag agtaaaataa gtataactca aagaataata aaaagcataa ttcaccaccg
     3001 gcactggcta tatatcttct gaaacttgat gtacctgaag gaaattacga ctcttctggc
     3061 agaagaatta ataaacaggc gcaagatgat gaaaaagaat tggggggcaa agtacaccct
     3121 gtgtcatctt caagcatcaa accggtagta gactactaca ttaatggaga taatctctat
     3181 gaaccattat caattccccc ttatggagtt agaaattata aagatacttt t
//
LOCUS       DAZGQL010000224         3211 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.226, whole genome shotgun sequence.
ACCESSION   DAZGQL010000224 DAZGQL010000000
VERSION     DAZGQL010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3211
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.226"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(14..324)
                     /locus_tag="ACHKIR_005124"
                     /pseudo
     CDS             complement(14..324)
                     /locus_tag="ACHKIR_005124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(327..494)
                     /locus_tag="ACHKIR_005125"
     CDS             complement(327..494)
                     /locus_tag="ACHKIR_005125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992715.1"
                     /translation="MPYTAACYSGFLTGSIRWAVYREIIRFSVEVEVEVEVEVEVEVE
                     VEVEVEGVYRS"
     gene            complement(726..1535)
                     /gene="iolB"
                     /locus_tag="ACHKIR_005126"
     CDS             complement(726..1535)
                     /gene="iolB"
                     /locus_tag="ACHKIR_005126"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HFO9992716.1"
                     /translation="MTNLLSTCTTRAGNIQHISPQNAGWEYVGFDVWLLKAGESLTLP
                     SDDRERCLVLVAGLASVKAADHFFYRIGQRMSPFERIPAYSVYLPHHTEAKVTAETDL
                     ELAVCSAPGFGELPVRLISPQDIGVEHRGKGRNQRLVHNILPDTQPADSLLVVEVYTR
                     EGDTSSWPAHKHDTAVEGQETYLEETYYHRFDPPQGFSLQRVYTADRSLDECMAVYNR
                     DVVKVPKGYHPVATIAGYDNYYLNVMAGPLRKWQFTWEADHAWINSSEYPR"
     gene            complement(1560..3065)
                     /gene="iolA"
                     /locus_tag="ACHKIR_005127"
     CDS             complement(1560..3065)
                     /gene="iolA"
                     /locus_tag="ACHKIR_005127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate-semialdehyde dehydrogenase IolA"
                     /protein_id="HFO9992717.1"
                     /translation="METVGNFIQGKTRLSNSGETLPVTNPATGEVIRLVTQSTRQDML
                     EAIQSAHEAFPAWSKMAPLRRARILFEFKVLLEKHRDELAALIVSEHGKVWSDALGEL
                     TRGIEVVEFACGIPHLIKGEFSFNVGTGVDSFSLMQPLGVVAGITPFNFPAMVPMWMF
                     PIALACGNTFVLKPPALVPSASLRMAQLLQEAGLPDGVFNVVHCGNDAASLLTSDPRI
                     QAVSFVGSSSVAEHIYTTASAHGKRVQAFGAAKNHAIVMPDADLDATVNAIMGGAFGS
                     AGERCMALPVVVAVGDETANRLIERLKPLIAALRIGPGELREKDENEMGPVVSRMHQQ
                     KVLAYIDKGVSEGATLVMDGRNYCVKGYPEGFYVGGTLFDNVTPEMTIWREEIFGPVL
                     GIVRVTDYATAIRTVNSHEFGNGSVIFTSNGHYAREFAQSVEAGMVGINVPVPVPMAF
                     HSFGGWKRSVFGALNVHGPDGVRFYTRMKTVTARWPNGQQIASEFSMPTLG"
BASE COUNT          852 a          797 c          734 g          828 t
ORIGIN      
        1 aacaaccatt cggctatatt atctttttca tgttggtgct tttattctgt gaggtgtgct
       61 ggcatggtgg acacaaccat ttgttctttt tagctgccat ttgataatat tttcctgaag
      121 ctttccgggg atatattccc cagacgggag tggcggcgct gacggttgta gaatatctca
      181 atgtataccc agtattactg agatgacttc attccggcta ctaaaatggt agtggttcag
      241 actttcattc ttcagagttc cccaaaagct ttccataggg gttttgtcgt aacagttccc
      301 tttacgagac attgatgttt tcagagctaa ctgcgataga ctccttcaac ttcaacttca
      361 acttcaactt caacttcaac ttcaacttca acttcaactt caacttcaac tgagaaacgg
      421 atgatttcac gatagactgc ccaacgtatt gagcccgtta ggaagccaga gtaacaagcc
      481 gccgtgtacg gcatttctct cctaattcgg cagaaccaaa aatattgtca gctcaggagt
      541 aagaatcaga aaaaaccata acagaacacc agaaacagga tattatcaga cagagaaaaa
      601 attcaaaaaa atccaccgat tctgttgcct gtggatttgt gtataagagg cagaaccctg
      661 ttggggactg tgcacaaaaa agccagctgc agaggacaac tggcaaagga atatttaacg
      721 tattgttaac gcggatactc tgacgaatta atccatgcgt gatcagcttc ccatgtgaat
      781 tgccatttgc gtaatggccc ggccatcaca ttcagataat agttatcgta acctgcgata
      841 gtggccaccg gatgatagcc tttcgggact tttaccacat cgcggttata tactgccata
      901 cattcgtcca gactgcgatc ggcggtgtag actcgctgta agctgaatcc ctggggtgga
      961 tcgaaacggt ggtagtaggt ttcttccaga taggtttcct gcccttcaac tgctgtgtca
     1021 tgcttatggg caggccagga acttgtatcg ccttcccttg tatatacttc cactaccagc
     1081 agactgtcag caggttgagt gtctgggagg atattatgaa ccaagcgctg attgcgccct
     1141 tttcccctat gttccacccc aatatcttgt ggtgagatca gtcttaccgg aagttcaccg
     1201 aatccaggag cactgcagac agccagttcc agatcagttt cggcagtgac ttttgcttct
     1261 gtatggtgcg ggaggtaaac agaataagcc ggaattcgct caaaaggact catgcgctgg
     1321 ccaatccgat aaaaaaagtg gtcagcggcc tttacgctgg caagtccggc aaccagaacc
     1381 aggcaacgtt cgcgatcatc ggatggcaag gttaaagatt cgccggcttt caacagccag
     1441 acatcaaaac cgacatactc ccagcctgcg ttttgcggag aaatatgttg aatatttcca
     1501 gccctggtgg tgcaggtact cagcaagttg gtcataatct tctccttcct gaacgtttat
     1561 taacctagcg taggcatact gaactcggat gcgatctgct gaccgttcgg ccagcgtgcg
     1621 gtaacggttt tcatccgggt ataaaaacgc accccatccg gtccgtggac attcagcgcc
     1681 ccaaacaccg agcgtttcca gccgccgaaa gagtgaaacg ccattggaac cggtaccgga
     1741 acattgatac cgaccatccc ggcttcaacc gattgagcaa attcgcgggc ataatggccg
     1801 ttggaggtga agatcacgct accattgcca aattcatgag agtttaccgt acgaatggcg
     1861 gtggcataat ctgttacgcg tacgatgccg agcacgggac caaaaatttc ctcacgccag
     1921 atagtcattt ccggcgtgac gttatcaaac aaggtaccgc cgacataaaa tccttcagga
     1981 tagcccttca cgcaataatt tcgcccatcc atcaccagtg ttgctccttc gctaacgcct
     2041 ttgtctatat atgcaaggac tttttgttga tgcatacgtg aaacaactgg ccccatttca
     2101 ttttcgtctt tttctcgtaa ctcaccgggg ccaatacgca gcgctgcaat aagtggtttt
     2161 aggcgttcaa taagacggtt ggctgtttca tcaccgacag cgaccaccac aggtaatgcc
     2221 atacaacgtt cgcctgcgga accaaaagcc ccacccataa tagcgttaac ggtagcatca
     2281 aggtcagcat ccggcatgac aatagcgtgg ttttttgcgg caccgaaagc ttggactcgc
     2341 ttaccgtgtg cactggcggt ggtgtagata tgctcagcga cggaagaaga tccgacaaaa
     2401 ctgacggcct gaatgcgtgg atcactggtc aacaggctgg ctgcgtcatt gccacagtga
     2461 actacattga atacaccatc cggtagtccg gcctcctgta gtagttgcgc catacgtaac
     2521 gatgcggagg gcaccagcgc tggaggcttc aggacgaagg tattgccgca ggcgagagcg
     2581 atagggaaca tccacattgg caccattgca gggaagttaa agggtgtgat gccagctaca
     2641 acaccaagtg gttgcattaa tgaaaagctg tcaacgcctg tgccaacatt aaaagagaac
     2701 tctcctttaa tgagatgcgg aatgccgcag gcgaattcaa caacttcgat tccgcgagtc
     2761 agttcaccaa gcgcgtcgga ccatactttg ccatgttcac tgacgatcag agccgccagt
     2821 tcatctcggt gtttttccag caacacttta aattcaaaaa ggattcttgc acggcgcaac
     2881 ggtgccattt tactccatgc aggaaaagct tcatgagcac tctgaatcgc ctccagcata
     2941 tcttgccggg tgctctgggt aaccaggcga atcacttctc cggttgcagg gttagtcacc
     3001 ggtagtgttt caccactgtt gctcagtctc gttttaccct gaataaaatt tccaactgtt
     3061 tccatcactg tttaccttct tgaatcaatg ttaacgacgt tccgactgag tgttcacagg
     3121 caaagcctga tcatctacag tctaaaatca cacagtgttt aatgtatagc ttcggattat
     3181 aaatttcaaa tttaaattta tgaaaaaata a
//
LOCUS       DAZGQL010000225         3134 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.227, whole genome shotgun sequence.
ACCESSION   DAZGQL010000225 DAZGQL010000000
VERSION     DAZGQL010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3134
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.227"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..1720)
                     /locus_tag="ACHKIR_005128"
     CDS             complement(<1..1720)
                     /locus_tag="ACHKIR_005128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992718.1"
                     /translation="MMNIRYPVRKADGRDYKSYDELLTDIRKNAHGWWLLGVNRYWHG
                     GIHVGRSSSPASVLNQETPEKSVPLQFMMDGEVVAWRVNRDYAAIECYQERPLRQSGT
                     FVLVKSVYKPDEQDESSWLTLYQLYMHIAPLSEFPKRPLYRVTQKGHGVRMRKYSRHD
                     DSREIAPDVLANKHGHPRTLTQGDTLAVLQQKSFLLKQRPESFALVQRLQDGKPAGEL
                     FWVSMRPEYLEPDGQCYVCLPDWMHSALNHGVFDDVVVPPVPLKVMVKAGDAVGFLGA
                     QDLADEDNYPQIITTDYKAHIELLSLDEHVPDVVANMKGIKTGKQFIKLKLKRPLYLR
                     NGEGEESTFEQMSAITRIDAGKIIPRDATYPFTDKTGITYFQIRPHTWMHQNDVEQLS
                     QHDLAGLNFHCIEAEHTTDFTRTLDERWLIDALKSISSHFDGEKGPQFAQAKMFYDSL
                     IHNAENRRAPNPYPDKSLDQYLFGALHTNQMNIPEYARRLIVKHDSCWHSTRDDTRWS
                     SVCKSRDESPVVKMANGGFLDATHWMDKVPPFASQRSVWHFHPLEFPEILRSAEFPRT
                     PINGVLT"
     gene            complement(2061..2275)
                     /locus_tag="ACHKIR_005129"
                     /pseudo
     CDS             complement(2061..2275)
                     /locus_tag="ACHKIR_005129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742560.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(2281..2967)
                     /locus_tag="ACHKIR_005130"
     CDS             complement(2281..2967)
                     /locus_tag="ACHKIR_005130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFO9992719.1"
                     /translation="MATMTDHQKEFISLFNRTACYHKRHKVFEDFVSCSVIALENRLQ
                     FSEAREQKYLRIVSGYEKQDVIHIAHLLAHVIEGLEQGFCDFLGSVFMQLELGDKYRS
                     QFFTPWSVASMMAQMQLGNVKALFESKPFITLSEPACGAGSMILAMVDTLNRSGYPAY
                     HRMWVSATDIDPLAAGMAYIQLSLCGVAGEVVIGNSLCNERRRVLLTPGHYLGNWDHR
                     LRHLQEQNVA"
BASE COUNT          765 a          815 c          724 g          830 t
ORIGIN      
        1 gagttaatac tccattaata ggagttctgg gaaactctgc acttcgtaat atttccggaa
       61 actcaagcgg atgaaaatgc catacagacc gctgactggc gaacggaggc accttatcca
      121 tccagtgtgt tgcatccaga aatcccccgt tagccatctt cacaaccggg ctttcatccc
      181 tggacttaca taccgatgac cagcgtgtat catcgcgggt actgtgccag cagctgtcgt
      241 gtttcactat caggcgtcgc gcatattccg ggatgttcat ctgattggta tgcagcgcac
      301 caaacaaata ctggtccaga gatttatcgg gatacgggtt tggcgctctt ctgttttcag
      361 cgttgtggat caggctgtca taaaacattt ttgcctgcgc gaactgcggt cctttttcac
      421 cgtcaaagtg cgagctgatg cttttcaggg catcaatgag ccagcgttca tccagcgtgc
      481 gggtaaaatc tgttgtgtgt tcagcttcaa tacagtgaaa atttagccct gctaaatcgt
      541 gctgactgag ctgctccaca tcgttctggt gcatccacgt atgtgggcga atctgaaagt
      601 atgtgatccc ggttttatcc gtaaacgggt acgtggcatc acgtgggata atttttccgg
      661 catcaattcg ggttatggcg ctcatttgtt caaatgtact ttcttcaccc tctccatttc
      721 gcaggtacag cgggcgtttc agcttcagct taatgaactg tttgcctgtt ttaatccctt
      781 tcatgtttgc caccacatct ggcacatgct catcgagact cagtagctcg atatgggctt
      841 tatagtccgt ggtaataatt tgcggatagt tatcttcgtc agccagatcc tgagcgccga
      901 ggaatccgac cgcatcccct gctttcacca tcactttcag tggcacaggc ggcaccacca
      961 catcatcaaa cacgccgtga ttcagcgcac tgtgcatcca gtcaggcagg cacacgtaac
     1021 attggccatc aggttccaga tattccggac gcatcgagac ccagaaaagt tctccggcgg
     1081 gtttaccgtc ctgaagacgt tgcaccagag caaacgactc cggccgctgt ttcagtaaaa
     1141 acgatttttg ttgcagtacg gcaagcgtgt caccctgcgt cagtgttctg gggtggccgt
     1201 gtttgtttgc cagtacgtcc ggcgcgattt cacggctgtc atcatggcgg gaatatttac
     1261 gcatccgcac accgtggcct ttttgtgtaa cccggtaaag cgggcgtttc ggaaattctg
     1321 acaatggcgc gatgtgcatg taaagctggt acagcgtcag ccaggatgat tcatcctgct
     1381 cgtctggctt gtacactgat tttaccagca caaaggtacc ggactggcgc agggggcgtt
     1441 cctgataaca ttcgatagcc gcgtaatcgc ggttaacccg ccatgccacc acttcgccat
     1501 ccatcatgaa ctggagcggc accgattttt caggtgtttc ctgattcagc accgaggccg
     1561 gggacgatga cctcccgaca tgaatacctc cgtgccagta acgatttacg ccaagtagcc
     1621 accaaccgtg ggcgtttttg cggatgtcgg ttagcagctc gtcatagctc ttatagtctc
     1681 gtccatcggc tttccgtacc ggataacgta tgttcatcat cttttcttaa gtgaaatcac
     1741 atatttcgat ggattctcgc gcgtattttg ggtttggggt gtagtggaac caaaaagacg
     1801 gttaagcggt taaattgaat ggtcaataac aggtaaatca atctgttgag atcaacgacg
     1861 tcttttgctc cgcaaacggc taagatgagt acagataatt tggttatcgt aaaaaaggtg
     1921 gctaaaaatt tcaacctgca atgtaaatta gccggttgat ttttggctat ttaaattgac
     1981 ctcttaacgg cgtttattgt tccattgctc cccaaccccc tatggattat aagccgcaca
     2041 tacattactt cgttctgcat atcaccacta atctgcttaa gatgtactaa caacatcatc
     2101 gcctgttggc taaacggaac tataactcct ctttcatttt catgccgcga tacaagtaat
     2161 gcacaccttt aatttcttca cgttttgccg gaatgcgcca aatagcgtta tcaaaatcaa
     2221 attcttttta acgagcaaaa cgccagtccg aagactggcg cagtgataaa acattctgca
     2281 tcatgccaca ttctgctcct gaagatgacg cagacgatgg tcccagtttc ccaggtaatg
     2341 cccgggagtc agcagtacgc ggcgtcgctc attacagagc gagttaccaa tcaccacttc
     2401 tccggccaca ccacacagcg acagttgtat atacgccatg ccggctgcca gcgggtcaat
     2461 atccgttgcc gacacccaca tcctgtggta tgccggatac cccgaacggt tcagggtgtc
     2521 aaccattgcc agaatcatac tgccggcacc acaggcaggt tcactgagcg tgataaaggg
     2581 tttactctca aacaacgcct tcacatttcc cagttgcatc tgtgccatca tgctggcaac
     2641 actccagggc gtgaaaaact gactccggta tttatccccc agttccagtt gcatgaacac
     2701 actacccaga aaatcacaga atccctgctc cagtccctca atgacatgag caagcaaatg
     2761 cgcaatgtgg ataacatcct gcttttcata accactgacg attcgcagat acttctgttc
     2821 ccgggcctca ctgaactgaa gccggttttc cagggcaatc acactacagc tgacaaagtc
     2881 ttcaaacacc ttatgacgtt tgtgataaca ggctgtccgg ttaaacaacg aaatgaattc
     2941 cttctgatgg tcagtcatgg tggccatgtt ttctccgtaa aaacagggaa cacatggccc
     3001 ggacagggga acaggtgttc cccttcgggt tgaaatgttg ataaaacggg catcacggac
     3061 ataatacccg gaggttaatt actgggctga gaccagagga accggtaaag gtgcggcttt
     3121 cgtctgttct tcag
//
LOCUS       DAZGQL010000226         3134 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.228, whole genome shotgun sequence.
ACCESSION   DAZGQL010000226 DAZGQL010000000
VERSION     DAZGQL010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3134
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.228"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            189..1325
                     /locus_tag="ACHKIR_005131"
     CDS             189..1325
                     /locus_tag="ACHKIR_005131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFO9992720.1"
                     /translation="MRKLSMVVCSVVSALMLCEATQAAEIYNKDGNKLDFYGRVKARH
                     YISDGSMDGDRTFARLGFKGETQINESLTGFGQWEYQFLGFKTESEVRNDKNRLAFAG
                     IRHNILGSLDYGRNYGVAYDVGALTDVLPDFGGDSWTYSDNYMTGRTTGVLTWRNTDF
                     FGLVDGLTFSAQYQGKNDRDNLLEANGDGYGFSVSYDYEGFGIVAAYTNADRTSLQEK
                     EGRKRFMTSKDSEGKEVITPRWIAGGKHAEFAGVGVKYNDNDLYLAADYSETRNMTPF
                     GSAGIADKARNIEVVAQYMFDFGLRPSLAYVQSKGMDVKGGIVNYGDQSLVEYIDVGA
                     NYFLNKNMSLLIDYKINLIDESEFTKKAGVATDNILAVGMMYQF"
     gene            1421..3118
                     /locus_tag="ACHKIR_005132"
     CDS             1421..3118
                     /locus_tag="ACHKIR_005132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HFO9992721.1"
                     /translation="MEHQLNKKLLNSSISLILASGLMMSTANAADDVKLKATKTNVAF
                     ADFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDDSSFDPKTMDNRNVVDTYKIGIDKAI
                     EAAKKSTPTLAALMDEGVKFTNGYVAHGVSGPSRAAILTARAPARFGVYSNSDAQDGI
                     PLEETFLSELLQNHGYYTAAIGKWHLSKISNVGVPEEKQTRDYHDNFTTFSAEEWQPQ
                     NRGFNYFMGYHASGVSLYNSPSLFRNRENIKAEGYISDQLTDEAIGVIDRASKLEQPF
                     MIYLAYSAPHLPNDQPAPKNYQEKFNTGSKTADNYYASIYSVDQGVKKILNTLKEKGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQAGYKGQPYPGGMHTPMFMWWNGKLKTGDYDKL
                     VSSMDFYPTALDAADIKIPASLKLDGVSLLPYVQKGADKKSAAGEPHKYLSWITSYSH
                     WWDEENTAFWNGYHKFVRKESDDYPHNPHTEDLSQFAYTVRSNDYSLVYTYAENKLAL
                     YDINDLQQTKDLSASHPDVVKNMKAVMIDFITSSQKPISDINQAKFENIKKSLTNVK"
BASE COUNT          986 a          612 c          666 g          870 t
ORIGIN      
        1 tgtgataaag gtcaagaaat aacacttgag aaagaaaagt caaatacact agaaaatata
       61 aaacctcaaa taaaaacaac aatatagatc actgagcaat attttgttac atctttgcat
      121 agtgataatt ttttcgccat agtttaattt ttaaaaccat actaaaaatg tgcacagggg
      181 atttttttat gcgaaaatta agtatggttg tgtgctctgt cgtttctgca ttaatgctgt
      241 gtgaggcaac acaggcagca gaaatctata ataaagacgg taataaactg gatttttatg
      301 gtcgcgttaa agcccgtcat tacatctctg atggttctat ggatggcgac agaacatttg
      361 cacgtcttgg tttcaaaggc gaaacacaaa ttaatgagag cctgactggc tttggtcagt
      421 gggaatacca gttcctgggg tttaagacag aaagtgaagt acgcaacgat aaaaatcgtc
      481 tggcatttgc aggtattcgc cacaatatac tcggttcact tgattatggg cgaaactatg
      541 gtgttgccta tgatgtgggg gctctgactg acgtacttcc ggactttggt ggtgacagct
      601 ggacttactc tgataactat atgaccggca gaacaaccgg agttctgacc tggcgcaata
      661 cagatttctt cgggcttgtt gatggactta ctttttctgc tcaatatcag ggtaaaaatg
      721 accgtgataa tttacttgaa gctaatggtg atggatatgg tttctccgta agctatgact
      781 atgaaggttt tggaatcgtt gccgcttaca ctaatgccga cagaaccagt ctgcaggaaa
      841 aagaaggccg taaacgtttc atgacatcga aagattcaga aggaaaagaa gttattacgc
      901 ctcgctggat tgccggggga aaacatgcgg agtttgcagg tgttggggtc aagtataatg
      961 ataatgacct gtatctggca gctgactact ctgaaacccg aaatatgact ccatttggta
     1021 gcgcaggcat cgcagataaa gcacggaata ttgaagttgt ggctcagtat atgtttgatt
     1081 tcggcctgcg tccttcactg gcttacgttc agtccaaagg gatggatgtt aaaggtggaa
     1141 tagtaaacta tggcgatcag tctcttgttg agtatattga tgttggcgca aactactttc
     1201 tgaacaaaaa catgtcactt cttattgatt acaaaattaa ccttattgat gaaagcgaat
     1261 ttaccaaaaa ggctggagtt gcaaccgata atatacttgc tgttggtatg atgtatcagt
     1321 tttaacaggt atccatttaa acgatttaca tagtttttat ttatatcaga ggcttttatt
     1381 attaataaat gttcgtgatg caactcagaa ggaataccat atggaacatc agttaaataa
     1441 aaaactgctt aactcatcaa tatcacttat attagcatct ggtctgatga tgagtactgc
     1501 aaatgcagca gatgatgtta agctcaaagc aacaaaaacc aatgttgcct ttgcggactt
     1561 cacgccgacg gaatacagca ctaaaggtaa gccaaatatc attgttttaa caatggatga
     1621 tttagggtat ggacaattgc cttttgatga tagttcattt gaccctaaaa cgatggataa
     1681 ccgaaatgtt gttgatacat acaaaatagg tattgataaa gcaattgaag ctgcaaaaaa
     1741 atccactcca acattagcgg ccctgatgga cgaaggtgta aaattcacta acggctatgt
     1801 tgcacacggt gtctctggcc cttcacgtgc cgcaatcctg acggcgcgag ctcctgctcg
     1861 cttcggtgta tattcaaatt ctgatgcaca ggatggtatc cctctggaag aaacattcct
     1921 gtctgaatta cttcagaatc atggttatta cactgcagca atcggtaagt ggcacttatc
     1981 aaaaatcagc aacgtaggtg tgcctgaaga aaaacaaacc cgtgactacc acgataactt
     2041 caccaccttc tccgcagaag aatggcaacc tcagaatcgt ggcttcaact acttcatggg
     2101 ttatcacgca tccggcgttt cactttataa ctccccgtca ttgttccgta accgtgaaaa
     2161 cattaaagct gaagggtaca ttagtgatca attaactgat gaagcaattg gtgtaattga
     2221 ccgtgcaagc aaactggaac agccatttat gatttatctg gcatacagtg caccacacct
     2281 gcctaacgat cagcctgcgc ctaaaaacta tcaggagaaa ttcaacactg gtagcaagac
     2341 tgctgataac tattatgcgt caatttactc cgttgaccag ggggttaaaa agatcctgaa
     2401 tacacttaag gaaaaaggtc agtacgacaa caccatcatt ctgttcacct ctgataacgg
     2461 tgctgtaatt gatggtccac tgccactgaa tggtgcgcag gctggttaca aaggccaacc
     2521 gtatccgggt ggtatgcata cacctatgtt catgtggtgg aatggcaagc tgaaaacggg
     2581 tgattatgac aaactggttt catcaatgga cttctatccg accgctttag atgccgcaga
     2641 tatcaagatc cctgcttctc tgaaactaga tggtgtatca ttattgccat atgtacagaa
     2701 aggagctgat aagaaatctg cagctggcga acctcacaaa tatctgtcct ggattacctc
     2761 ttattctcac tggtgggacg aagagaacac tgcattctgg aacggatatc ataaattcgt
     2821 gagaaaagag tctgatgatt atccgcacaa cccacataca gaagacttaa gccagtttgc
     2881 atacactgta agaagcaacg attattctct ggtttacacc tatgcagaga ataaactagc
     2941 tctgtacgat atcaacgatc tgcaacaaac taaagatctc tctgcatccc atcctgatgt
     3001 agttaagaat atgaaggctg ttatgattga cttcatcacg tcaagtcaaa aaccgatcag
     3061 tgatatcaac caagccaaat ttgagaatat aaaaaaatct ttaactaacg tgaaatgata
     3121 ataagcaggg agta
//
LOCUS       DAZGQL010000227         3124 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.229, whole genome shotgun sequence.
ACCESSION   DAZGQL010000227 DAZGQL010000000
VERSION     DAZGQL010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3124
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.229"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..193)
                     /locus_tag="ACHKIR_005133"
     CDS             complement(<1..193)
                     /locus_tag="ACHKIR_005133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFO9992722.1"
                     /translation="MFFRGHALMKTKKHTSRTATKTPERDLYQLVTDRVVTAIENGVP
                     PWKRPWRSARNGVGLPEMMP"
     gene            complement(262..645)
                     /locus_tag="ACHKIR_005134"
     CDS             complement(262..645)
                     /locus_tag="ACHKIR_005134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992723.1"
                     /translation="MGLDIYFLARDNHHSGAVVGTQEHQEVGAFRKVKPLLTWFREHT
                     GASDEQHAFPVTRSSLAALLRDLSMLTPGNCHRLFPYVTGSCLRGEDYAGDYWYDVDD
                     VKAWVSNTLKTFDFERRSLVMLALW"
     gene            complement(761..1390)
                     /locus_tag="ACHKIR_005135"
     CDS             complement(761..1390)
                     /locus_tag="ACHKIR_005135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992724.1"
                     /translation="MSELSSRPAREPVVYTLEQVSTIPEKQWHAFVLAVTETFWQLPE
                     ALRPQNAYFGSLTRASELFPVTDTLAFYCRSADGLWSVNVTIEREHSRNILALNELNF
                     GRQPGDFFARTVFVLLHNLCPDCFRIHSTVGGASCSLPLKWIKRYLGHENFSAPESVL
                     TTPVRGDAFDSLLLQFLSGQGRQLSPDDWAALEEAEYQLYWLRALAGGR"
     gene            complement(1472..1849)
                     /locus_tag="ACHKIR_005136"
     CDS             complement(1472..1849)
                     /locus_tag="ACHKIR_005136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000287229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992725.1"
                     /translation="MYSPLIAHYSALQTQSALLAHISQMEGELMRLRAWQRLGITPEP
                     DDETEPSLVYVYLWDTGEALGWLLYDLEQEEISAPGIQARQALDSLMALGREINHEIW
                     ADSISTGKMVAGADEYFARHDMM"
     gene            complement(2045..2524)
                     /locus_tag="ACHKIR_005137"
     CDS             complement(2045..2524)
                     /locus_tag="ACHKIR_005137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001426131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992726.1"
                     /translation="MHSLLILLYVVVWRACTILRVIAPVSAVRAWCEYQLEGMTILRD
                     AYSSLAKAAGMQNDAFEYTESIARHLGYKSGGDQLKSGLFRPFLWHIRVKGHRLGAVM
                     EMEAWKTWKNGCNDVCNPWEDVCNPWERGWNDGWTECLNQRIAGLLKQACIEFSDAD"
     gene            complement(2607..2999)
                     /locus_tag="ACHKIR_005138"
     CDS             complement(2607..2999)
                     /locus_tag="ACHKIR_005138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992727.1"
                     /translation="MSTFILYPEPGISSFFRRMTAQLRVFFSRNNLSYYSRWYTNPAG
                     NLSVRVCMFRRGGVWTPCLYYRQGMYRNEYTGWYQPLPREVIYLNRFTGRISRRRTWH
                     AACRMAEQLTTLRYRFGLQGDGIVAQPC"
BASE COUNT          853 a          901 c          728 g          642 t
ORIGIN      
        1 ccggcatcat ctccggaagc cccacgccat tacgcgcaga gcgccaggga cgtttccagg
       61 gtggcacacc attttcaatg gccgtaacaa ccctgtcggt cacaagctga tacaggtcac
      121 gttccggagt tttagtcgcg gtacgtgaag tatgtttttt cgttttcata agagcatgcc
      181 ccctgaaaaa gatgcacgct ccctgtacgg tgagagtgca ccgtacagtt aaattaataa
      241 cgaaaatcag actggccaga attaccacaa cgccagcatc accagtgaac gacgctcaaa
      301 atcaaacgtc ttcagcgtat tgctgaccca cgcttttacg tcatccacgt cataccagta
      361 atcaccggca taatcttccc cccgtaaaca cgagcctgtg acataaggaa acagcctgtg
      421 gcagttcccc ggtgtcagca tactgaggtc gcgcaataat gcagccagtg aactgcgggt
      481 tacagggaaa gcatgctgct catctgaagc accggtatgt tcacgaaacc atgtcagaag
      541 cggtttcacc ttacggaaag cccccacctc ctgatgttcc tgtgttccca caacggcacc
      601 actgtgatgg ttatcacgag ccagaaagta aatatcgagt cccataacaa ttctcctgaa
      661 taaagccggg actctcccca cagggatatg tccctgccgg gtgatataat gacggaatac
      721 gtgatgacgt tattccggat aaagaaacta cggcgttctg ttaccgccca ccggcaagag
      781 cccgcagcca gtaaagctga tattcggcct cttccagtgc tgcccagtca tccggggata
      841 actgtcgccc ctgaccggac aaaaactgca gcagtaagct gtcaaacgca tctcccctta
      901 ccggggtggt taatacagac tccggagcag aaaaattctc atgccccagg taccgtttta
      961 tccatttcag cggcagcgag cagctggcac caccgacagt ggaatgaata cgaaaacagt
     1021 ccggacacag attgtgcaac agcacaaaga ctgtacgggc aaagaaatct cccggctgac
     1081 gtccgaaatt cagctcatta agagccagaa tattccggct gtgctcccgc tcaatagtga
     1141 cattcaccga ccacagcccg tcggcagacc ggcagtaaaa cgccagcgtg tccgttaccg
     1201 gaaacaactc tgacgcccgg gtcagggagc cgaaatacgc attctgcggc cgtaacgcct
     1261 ccggcaactg ccagaaggtt tcagtgacag ccagaacaaa agcatgccac tgcttttccg
     1321 gtattgtgga aacctgctca agggtatata ccaccggctc acgggccgga cgtgaagata
     1381 actcagacat ggaacattcc tcatggttaa aacgaaacaa cggggaatgt ctcccggacg
     1441 gagaactccc ccgtcagggt caggtcatga attacatcat gtcgtgccgg gcaaaatatt
     1501 catccgctcc ggccaccatc tttccggtac tgatactgtc agcccagatt tcatgattaa
     1561 tttcacgccc cagagccatc agactgtcga gtgcctgacg ggcctgaatt cccggtgcgg
     1621 aaatctcctc ctgctccagg tcatacaaca gccagcccag cgcctcgccg gtatcccaca
     1681 gatacacgta taccagtgaa ggctccgtct catcatccgg ctccggggta atacccagcc
     1741 gttgccaggc acgtaaccgc atcagctcgc cttccatctg actgatatga gccagcagag
     1801 cagactgcgt ctgaagcgct gaataatgag caattaaagg tgaatacatg atgacctccg
     1861 tctcttaaag gaagtcctgt ccctgatgga acaaaactcc cgtcagggaa aacaccggag
     1921 caaaaacacg gtgcagtaag taaaagaaaa accgttacgg gagcaaaaca gccatcattg
     1981 caaaatggca gttgcatcgc tcacgatttg acatccttcc tgtgactgac tggcatcctg
     2041 cccgtcagtc tgcgtcggaa aattcaatac aggcctgctt caggagcccg gctatacgtt
     2101 gattcagaca ttccgtccag ccatcgttcc atccacgctc ccatggattg cagacatcct
     2161 cccatggatt gcagacatcg ttacagccat tcttccatgt cttccatgcc tccatctcca
     2221 tgacagcccc cagcctgtgg cctttcacac gtatatgcca cagaaacggc cggaaaagtc
     2281 ctgatttcag ttggtcacca ccgcttttgt agcccagatg acgtgcaata ctttcagtat
     2341 attcaaatgc atcattctgc attccagccg ccttagccag cgagctgtac gcatcacgaa
     2401 gtatcgtcat accttccagt tgatactcac accacgcacg gacagccgac actggtgcga
     2461 ttacccgaag aatggtgcag gcacgccata caacaacgta aaggagtatt aacagactat
     2521 gcatgtgttc acctcagata caaaaaataa cgggaacaca cctgccctgc gggaagcgtg
     2581 ttcccgacag ggtaataaat gataaatcag cagggctgtg cgacgattcc atcaccctgc
     2641 agaccaaacc ggtaacgcag ggtggtcagt tgctccgcca tacggcaggc agcatgccat
     2701 gtcctgcgac gggatatccg tccggtaaac cggttcagat aaatgacctc acgcggtaac
     2761 ggctgatacc atcctgtgta ctcgttgcga tacattccct gccggtaata caggcagggt
     2821 gtccagacac cgccacgacg gaacatacag acccgcacgc tcagattgcc ggccggatta
     2881 gtgtaccagc gactgtaata tgacaggtta ttgcgggaaa aaaacacccg caactgagct
     2941 gtcattcgcc ggaaaaaaga agagatgccc ggttcagggt acaaaataaa cgttgacata
     3001 aagactcctg taaaaaaacg ggagccttcg ccccgacgga caaaaactcc cgtcagggtt
     3061 aaaacaccac cagtacagca tggtgttgct gtcagaaaaa gcccgttaat aaagggcgca
     3121 gtta
//
LOCUS       DAZGQL010000228         2996 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.230, whole genome shotgun sequence.
ACCESSION   DAZGQL010000228 DAZGQL010000000
VERSION     DAZGQL010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2996
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.230"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            398..1123
                     /locus_tag="ACHKIR_005139"
     CDS             398..1123
                     /locus_tag="ACHKIR_005139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO9992728.1"
                     /translation="MLPGCCKNGIVISKIPVMQAGLKEVMRTHFPEYEIISSASAEDL
                     TLLQLRRSGLVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELLM
                     CPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTLSERK
                     VLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGARELN
                     LPSVYGDAAEWNTAELRREMSHS"
     gene            1081..1758
                     /gene="bglJ"
                     /locus_tag="ACHKIR_005140"
     CDS             1081..1758
                     /gene="bglJ"
                     /locus_tag="ACHKIR_005140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HFO9992729.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(1795..2583)
                     /gene="fhuF"
                     /locus_tag="ACHKIR_005141"
     CDS             complement(1795..2583)
                     /gene="fhuF"
                     /locus_tag="ACHKIR_005141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HFO9992730.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRATIAEHREHLLEFI
                     RLDEPAPLRAMTLAQWSSPNTLSSQLAVYSDHIYRNQPLMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHVEFHETGRAACFWVDVCEDKNATLHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     MLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            2724..2960
                     /locus_tag="ACHKIR_005142"
     CDS             2724..2960
                     /locus_tag="ACHKIR_005142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HFO9992731.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIIILMGLVLTP
                     AMLYHKQLRHYVLLPSCLALIAGIMLMIMNLKQG"
BASE COUNT          812 a          621 c          731 g          832 t
ORIGIN      
        1 aatcaggata aaagataact gggcgcgttt tttaattttt acaagttaaa actatcagat
       61 tttattgaat aaatattgat tgtgtgattt tgcattctgc tggaagggat atcagttaat
      121 tataaattaa atgaatgaga attctgtttt tcattaagta tttctaacaa cttaatgtag
      181 atattttaaa tgtctccagg ctatttcacc aggttatcat ttgatgctaa ccttctgaca
      241 catgcagtgg agttgttgtg cagcaggagt atgctgatat gaaagtgaat gctaaggata
      301 atttattcgc ttaatctatt aatttgctgg aaacatttaa ggaatgaata tcctgaataa
      361 accatcatga tttttagaca gtggatgtgg aggaaatatg ttgccaggat gctgcaaaaa
      421 tggaattgtt atcagtaaaa tacctgttat gcaagcaggg ttaaaagagg tcatgaggac
      481 tcacttccct gaatatgaaa taatatccag cgcctctgcg gaggacctta ccttattaca
      541 attacgtcgt tccggattag tcattgctga tttagccggt gaaagtgaag atccacgttc
      601 tgtttgtgaa cattattatt ctttaatctc acaatatcgg gaaattcact gggttttcat
      661 ggtgtcacgc tcctggtatt cccaggcagt agaactgctt atgtgcccta cggcgacgtt
      721 attgtctgat gttgaaccca ttgagaatct ggtcaagacc gtacgttccg gcaatacgca
      781 cgcagagcgt atcagcgcca tgctgacctc tccggcaatg actgaaactc atgattttag
      841 ctatcgctcc gtcattctca ctctttcaga gcgcaaggta ctgcggctat taggtaaagg
      901 atggggcatc aaccagatag cttcattgct taagaaaagt aataaaacta tcagcgccca
      961 aaaaaacagt gcgatgcgtc gactggcaat tcacagcaac gctgaaatgt atgcatggat
     1021 aaatagcgcg cagggtgcaa gagaacttaa cttgccttct gtttatggag atgccgcaga
     1081 atggaacaca gccgaattaa gaagagaaat gtcgcactca tagaaaaatg cgtcatgagc
     1141 agtatcggta ttgagagttt attcagaaag tttgcgggta acccttataa gctccatacc
     1201 tataccagtc aggagtcatt tcaggatgcc atgtcgcgga tctcgtttgc ggcggtcatt
     1261 ttttcttttt ctgccatgag aagtgagcgc agagagggat tatcttgcct gactgaactg
     1321 gcgattaagt ttccgcgtac ccggcgttta gttattgcgg atgatgatat tgaagctcgg
     1381 ctgattggtt cattgtcgcc atcaccgttg gacggtgtat taagtaaagc gtcaacgctg
     1441 gagatttttc atcaggaact ctttttgtca ttaaatggtg tacgtcaggc gaccgaccga
     1501 ctgaacaatc agtggtacat taatcaaagc cgaacgctaa gcccgacgga gagagaaata
     1561 ttgcgcttta tgtcgcgtgg ctactcaatg acacaaattg ccgagcagct taaacgcaat
     1621 atcaaaacga tccgtgcaca taaatttaat gtgatgtcga aactgggcgt cagttctgat
     1681 gcagggttgt tggaggccgc agatattctg ttatgtatgc ggcattgcga agcaagtaat
     1741 gtgttgcatc cctattaatc cacatgatgc cgggtttact tccccggcag tgcttcattt
     1801 cagcgtacaa tcgccacatt gctgcacatc cggtaagcga taacgctggc agcaagtgcg
     1861 gcgcaccagc aggccgtcgc gcagtaccac ggtacgccag agtggattat cttcaccgtt
     1921 cgtgagcatt ttctcaaaaa agagggcatg gcgcagcgat tcaaccgtcg cctcgccaag
     1981 cagctgtttc atctcagtca gataccagtt gatcaaataa ccggtattgc tccagataag
     2041 tttgccgttg atctctcccg tcgcttctaa tgcctgcact accggaacaa gcgcctggct
     2101 gattaacgtt tccattcgct gctgcggcga atgtagtgtt gcgtttttat cttcacacac
     2161 atcgacccag aaacaggcgg cgcgtccggt ttcgtgaaac tcaacgtgga aatgttccgg
     2221 cgacacatct aatgcttttt cctgcgtcag cagcgccaac attaatggtg gcaccatcag
     2281 gccgatatac cattgtgccc atagtgagat cagcggtttg ttctcgcgga tcatcaaggg
     2341 ttggttgcgg tagatatgat cggaatagac cgccagttga gaacttagcg tattcggtga
     2401 tgaccattgc gccagcgtca tggcacggag cggtgcgggt tcatccagac ggataaactc
     2461 cagcaaatgt tcacgatgtt cggcgatcgt cgcccgtacg gcttgtgcaa gcccagcatc
     2521 ctgcggctgg agatgcgttc gccagataac atcttcatag agcggtgcgg aacgataggc
     2581 cataatcgtg atagtaatct aaatgataat gattgctaat cctagcgata ggtttacccg
     2641 atagcaaggg atttctctgg cttgcaaatg ataaaaatta tcatatgata ttggttatca
     2701 ttatcaatga aagagatgaa accatgttgc aacgtacgct gggcagtggc tggggagtgt
     2761 tgctgccggg attgctgatt gcagggctga tgtatgcgga tttatcgccg gatcaatggc
     2821 ggattatcat tctgatggga ttagtattga cgccggcgat gctgtatcac aaacagttgc
     2881 ggcattacgt tttgctgcca tcgtgcctgg cgcttattgc tggcatcatg ctgatgataa
     2941 tgaatttgaa gcagggatga taaaacaggg aagaaacaag aaaggaagta aagata
//
LOCUS       DAZGQL010000229         2994 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.231, whole genome shotgun sequence.
ACCESSION   DAZGQL010000229 DAZGQL010000000
VERSION     DAZGQL010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2994
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.231"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            280..2955
                     /gene="adhE"
                     /locus_tag="ACHKIR_005143"
                     /gene_synonym="adhC"
     CDS             280..2955
                     /gene="adhE"
                     /locus_tag="ACHKIR_005143"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFO9992732.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVAYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
BASE COUNT          782 a          751 c          733 g          728 t
ORIGIN      
        1 atggcgaaaa gcgatgctga aaggtgtcag ctttgcaaaa atttgatttg gatcacgtaa
       61 tcagtaccca gaagtgagta atcttgctta cgccacctgg aagtgacgca ttagagataa
      121 taactctaat gtttaaactc ttttagtaaa tcacagtgag tgtgagcgcg agtaagcttt
      181 tgattttcat cggttaagca aatcatcacc gcactgacta tactctcgta ttcgagcaga
      241 tgatttacta aaaaagttta acattatcag gagagcatta tggctgttac taatgtcgct
      301 gaacttaacg cactcgtaga gcgtgtaaaa aaagcccagc gtgaatatgc cagtttcact
      361 caagagcaag tagacaaaat cttccgcgcc gccgctctgg ctgctgcaga tgctcgaatc
      421 ccactcgcga aaatggccgt tgccgaatcc ggcatgggta tcgtcgaaga taaagtgatc
      481 aaaaaccact ttgcttctga atatatctat aacgcctata aagatgaaaa aacctgtggt
      541 gttctgtctg aagacgacac ttttggtacc atcactatcg ctgaaccaat cggtattatt
      601 tgcggtatcg ttccgaccac taacccgact tcaactgcta tcttcaaatc gctgatcagc
      661 ctgaagaccc gtaacgccat tatcttctcc ccgcacccgc gtgcaaaaga tgcaaccaac
      721 aaagcggctg atatcgttct gcaggctgct atcgctgccg gtgctccgaa agatctgatc
      781 ggctggatcg atcaaccttc tgttgaactg tctaacgcac tgatgcacca cccagacatc
      841 aacctgatcc tcgcgactgg tggtccgggc atggttaaag ccgcatacag ctccggtaaa
      901 ccagctattg gtgtaggcgc gggtaacacg ccagttgtta tcgatgaaac tgctgatatc
      961 aaacgtgcag ttgcatctgt gctgatgtcc aaaaccttcg acaacggcgt aatctgtgct
     1021 tctgaacagt ctgttgttgt tgttgactct gtttatgacg cagtacgtga acgtttcgca
     1081 acccacggcg gctatctgtt gcagggtaaa gagctgaaag ctgttcagga tgttatcctg
     1141 aaaaacggtg cgctgaacgc ggctatcgtt ggtcagccag cctataaaat tgctgaactg
     1201 gcaggcttct ctgtaccaga aaataccaag attctgatcg gtgaagtgac cgttgttgat
     1261 gaaagcgaac cgttcgcaca tgaaaaactg tccccgactc tggcaatgta ccgtgctaaa
     1321 gatttcgaag acgcggtaga aaaagcagag aaactggttg ctatgggcgg catcggtcat
     1381 acctcttgcc tgtacactga ccaggataac caaccggctc gcgttgccta cttcggtcag
     1441 aaaatgaaaa cggcccgtat tctgatcaac accccggcgt ctcagggtgg tatcggtgac
     1501 ctgtataact tcaaactcgc accttccctg actctgggtt gtggttcctg gggtggtaac
     1561 tccatctctg aaaacgttgg tccgaaacac ctgatcaaca agaaaaccgt tgctaagcga
     1621 gctgaaaaca tgttgtggca caaacttccg aaatctatct acttccgccg tggctctctg
     1681 ccaatcgcgc tggatgaagt gattactgat ggccacaaac gtgcgctcat cgtgactgac
     1741 cgcttcctgt tcaacaatgg ttatgctgat cagatcactt ccgtactgaa agcagcaggc
     1801 gttgaaactg aagtcttctt cgaagtagaa gctgacccga ccctgagcat cgttcgtaaa
     1861 ggtgcagaac tggcaaactc cttcaaacca gacgtgatta tcgcgctggg tggtggttcc
     1921 ccgatggatg ccgcgaagat catgtgggtg atgtacgagc atccagaaac tcacttcgaa
     1981 gaactggcgc tgcgctttat ggatatccgt aaacgtatct acaagttccc gaaaatgggc
     2041 gtgaaagcga aaatgatcgc tgtcaccacc acttctggta caggttctga agtcactccg
     2101 tttgcggttg taactgacga cgctactggt cagaaatatc cgctggcaga ctatgcgctg
     2161 accccggata tggcgattgt cgatgccaac ctggttatgg acatgccgaa gtccctgtgt
     2221 gctttcggtg gcctggacgc agtaactcac gccatggaag cttatgtttc tgtactggca
     2281 tctgagttct ctgatggtca ggcactgcag gcactgaaac tgctgaaaga atatctgcca
     2341 gcgtcctacc acgaaggatc taaaaatccg gtagcgcgtg aacgtgttca cagtgcagca
     2401 actatcgcgg gtatcgcgtt tgcgaacgcc ttcctgggtg tatgtcactc aatggcgcac
     2461 aaactgggtt cccagttcca tattccgcac ggtctggcaa acgccctgct gatttgtaac
     2521 gttattcgct acaacgcgaa tgacaacccg accaagcaga ctgcattcag ccagtatgac
     2581 cgtccgcagg ctcgccgtcg ttatgctgaa attgctgacc acctgggtct gagcgcaccg
     2641 ggcgaccgta ctgctgctaa gatcgagaaa ctgctggcat ggctggaaac gctgaaagct
     2701 gaactgggta ttccgaaatc tatccgtgaa gctggcgttc aggaagcaga cttcctggcg
     2761 aacgtggata aactgtctga agatgcattc gatgaccagt gcaccggcgc taacccgcgt
     2821 tacccgctga tctccgagct gaaacagatt ctgctggata cttactacgg tcgtgattat
     2881 gtagaaggcg aaactgcagc gaagaaagaa gccgctccgg ctaaagctga gaaaaaagcg
     2941 aaaaaatccg cttaatcagt agcgctgtct ggcaacataa acggcccctt ctgg
//
LOCUS       DAZGQL010000230         2992 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.232, whole genome shotgun sequence.
ACCESSION   DAZGQL010000230 DAZGQL010000000
VERSION     DAZGQL010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.232"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            367..885
                     /locus_tag="ACHKIR_005144"
     CDS             367..885
                     /locus_tag="ACHKIR_005144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFO9992733.1"
                     /translation="MPTPCYISITGQTQGNITAGAFTADSVGNIYVQGHEDEMLVQEF
                     LHNVTVPTDPQSGQPAGQRAHKPFIFTVALNKAVPLMYNALASGEMLPTTELHWWRTS
                     VEGKQEHYFTTRLTDSTIVDMKLHMPHCQDPAKREFTQLLEVSLAYRKIEWEHVKSGT
                     SGADDWRAPLEA"
     gene            1095..>2992
                     /locus_tag="ACHKIR_005145"
     CDS             1095..>2992
                     /locus_tag="ACHKIR_005145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HFO9992734.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTVWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVCPPLWRT
                     GLRQNFRIFQNEDIESILATILKENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAQKSTDQSLVLCDTVRYLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFDQEGQHQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAEVARGTSRSPEIWPGRRIALTGHPQANLNREWQVVASELHGEQPQAVPGR
                     RGSGTTLNNHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPSNQDSSCWIRVAQAWAGTGFGNLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFDDATGKEQVYIHAQKNMNTEVLNN
                     RTTDVINNHAETIGNNQVIAVTNNQIQTVGVNQIETVGSNQIIKVGSVQVETIGLVRA
                     LTVGVAYQTTVGGIMNTSVALMQSSQIGLHKSLMVGLGYDVKVGNNVTFTVGKTKKDD
                     TGQTAIYSA"
BASE COUNT          762 a          737 c          832 g          661 t
ORIGIN      
        1 atatttgttt tgtgattgat taagtgggta tttttaagga atatctcaaa aattatcaaa
       61 atgataattc aaaaaggata aatacaatat ttattcatga ttcagctttg gccattttat
      121 ggaggcaatt tggactttga acagttgggt atggttatcc agatatatat tattaactta
      181 ttgctgaata agagatttaa tgattttaaa aatagttttt acgacaaccg taaattctag
      241 ctcggtttgt atgtaaaaaa tactgaaaaa tgatgcggtt ccgtgaagag agctgcatct
      301 gcctgtctgg gattaacggc aggtcattgt aagtagtaat taaccacgtt aacaaggagt
      361 ttacaaatgc caacaccgtg ttatatctcc atcactggtc agacccaggg aaatattact
      421 gcaggtgcat ttaccgctga ttctgtcggc aacatctatg tgcaggggca cgaagacgag
      481 atgctggtgc aggaattcct gcataacgtt actgtcccga ccgatcctca gtccggccag
      541 cctgcgggtc agcgtgctca caagccgttc atttttaccg tggcgctgaa caaagctgtt
      601 ccgctgatgt acaacgcact ggcctccggc gaaatgctgc caaccactga acttcactgg
      661 tggcgtacct ctgtggaagg caaacaggag cattatttca ccacccgcct gaccgattcg
      721 acgattgtcg atatgaaact gcatatgccg cattgccagg atccagcgaa acgcgagttc
      781 acccagttgc tcgaagtttc actggcctac cgcaaaatag agtgggaaca cgtcaagtct
      841 ggcacctccg gtgccgacga ctggcgcgca ccgctggaag cataagttaa gccaacagca
      901 tccggctatc aggccggatg ctgtttttgt attacgccgt aaaaagaaag gttgtcagta
      961 agggattacg ttcacgggag atggcggatg ccggaaactt cggcggtagc gtgcctgaac
     1021 gcaagcgtaa cactgtactg ttacagccgc tttaacagcg aactgtctgt ttttccttaa
     1081 acccggaggt tgctatgtca accggattac gtttcacgct ggaagtggac ggtctgccac
     1141 cggacgcgtt tgcggtggtc tcctttcatc tgaaccagtc actctcttcg ctcttttccc
     1201 tcgatctctc tctggtcagc cagcagtttc tctcccttga atttgcgcag gtgctggaca
     1261 aaatggccta cctgacggta tggcagggcg atgacgtaca gcgccgggtg aaaggtgtgg
     1321 tgacctggtt tgaactgggg gagaacgaca aaaaccagat gctgtacagc atgaaggtgt
     1381 gcccaccgct gtggcgcaca gggctgcgcc agaacttccg tatcttccag aatgaggaca
     1441 tcgaaagcat actcgcgacg atcctgaaag aaaacggggt gaccgagtgg agtccgctgt
     1501 tcagcgagcc gcatccttcc cgtgagtttt gtgtccagta cggcgaaacc gattacgatt
     1561 tcctgtgccg gatggcggcg gaggaaggca tcttctttta tgaggagcac gcgcaaaaaa
     1621 gtaccgacca gagcctggtc ctgtgcgaca ccgtgcgtta tctgccggag tcctttgaga
     1681 tcccctggaa tccgaacacc cgtaccgagg tgagcaccct atgcatcagc cagttccgct
     1741 acagcgcaca aatccgccca tcttccgtgg tgaccaaaga ctacaccttt aaacgacccg
     1801 gctgggcagg gcgttttgat caggaaggcc agcaccagga ttaccagcgc acacagtatg
     1861 aagtgtatga ctaccccgga cgtttcaagg gtgcccacgg gcagaacttt gcccgctggc
     1921 agatggatgg ctggcgaaac aacgcagaag tggcgcgcgg aacaagccgt tcgccggaga
     1981 tatggccggg acggcgaatt gcgctgacgg ggcatccgca ggcgaacctg aaccgggaat
     2041 ggcaggtggt ggcaagtgaa ctgcacggcg agcagccgca ggcggtgccg ggacgcaggg
     2101 gttcaggtac cacgctgaat aaccactttg cggtaatacc ggcagacaga acatggcggc
     2161 cacagccgtt gctgaaaccg ctggtggacg gcccgcagag cgccgtcgtg acgggaccgg
     2221 caggagaaga aatcttctgt gatgaacatg gccgcgtgcg ggtgaaattt aactgggacc
     2281 gctataaccc gtcaaaccag gacagttcat gctggatccg tgtggcacag gcgtgggcag
     2341 gcaccggctt tggcaacctg gcgataccgc gtgtgggtca ggaagtcatt gtggacttcc
     2401 tcaacggcga tccggaccag ccgattatta tggggcgtac ctaccaccag gaaaaccgca
     2461 cccccggcag cctgccggga acaaagacgc agatgaccat tcgttcgaaa acctataagg
     2521 gcagcgggtt taatgaactg aagtttgacg atgcgacagg gaaagaacag gtctacatcc
     2581 acgcgcaaaa gaacatgaac accgaggtgc tgaataaccg gaccacggat gtgataaaca
     2641 accatgctga gaccatcggc aacaatcagg tgattgcggt taccaacaat cagatacaga
     2701 cggtgggcgt taaccagata gagacggtgg gcagtaacca gatcattaaa gtgggctctg
     2761 ttcaggtgga aacgattgga cttgttcgtg cgctgaccgt gggcgtggcg tatcagacga
     2821 cggtaggtgg cattatgaac acctcggtgg ccctgatgca gtcctcgcag atcggtttgc
     2881 ataaatcgtt gatggtcggg ctgggttatg acgtcaaagt cggaaataac gttaccttca
     2941 ccgttggtaa aacgaaaaag gatgataccg gacagaccgc gatttactcc gc
//
LOCUS       DAZGQL010000231         2945 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.233, whole genome shotgun sequence.
ACCESSION   DAZGQL010000231 DAZGQL010000000
VERSION     DAZGQL010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2945
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.233"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..840
                     /locus_tag="ACHKIR_005146"
     CDS             <1..840
                     /locus_tag="ACHKIR_005146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2345 domain-containing protein"
                     /protein_id="HFO9992735.1"
                     /translation="LENAAERAQALKADIDSQIQMFEQRLKPLNEAVLFSAPEGIALT
                     SGEDVQLAAGQNLAVNAGGDISTGTMGNLTALAGERLGLFARSGQLSVKSGEGPVEVQ
                     AQNGSMRLFAEKKLTLSSASDISFAGKKRITLIGGGSYLRLEAGKIEYGTTVSYLRKV
                     KRTIAAGAASQQPEMPWLADPVKPSASALMQYLYHDDVPVVGAPFKATLADGTVREGN
                     LDGAGYLHLEDVPPGRVLVELGPDTRDYLRKNKTDNRLFVGTSLSEADIDSLIAKYSE
                     GVE"
     gene            837..2381
                     /locus_tag="ACHKIR_005147"
     CDS             837..2381
                     /locus_tag="ACHKIR_005147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002445136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992736.1"
                     /translation="MSGVNVAWFKETSLETGQWIWGTLQGAFNEKQTIGQIIVDAVIG
                     MIPLVGDVTAVRDLIAVSIGLAKEPKKRESVTEWVFLVILLFALIPVIGGVIKGVGRL
                     ALRVTTKVAENVETLGAVIKFLNRIGEGDAVKWLNKLNVVEYQAQLIEKMGDFCNTMI
                     TAINKILKARVGRMLPEKWQADLLLVRDGFAALQRLASRMIPQALKELDAKLRVLQAL
                     VYKGEIHTITTGMGRAEVRREAEAYLIERPLCEAPKQGTRYPSVHAEVIHEDRLRLKY
                     SREGYPDLLGYKGKSPAFGDEQVFTNIASFSGEITAKSASEMGGSTIFRAFGNPTELA
                     PNGSWAAGGFWGYQKVPGSAEEWRQLSAVLDEWNGNGFLVVLHLPEDLATRMPGSNAW
                     HGKIAEQFGQKRPDQYLQGGGEQVIINLDETLKTELNRIGEQVKASGQAVSIEINGVN
                     AEFYPTGWKEVEGVHGYSKPEGIEPPYVTYTRELHDDELRSKVTERTILNAARIEYQT
                     TTGSEQ"
     gene            2378..>2945
                     /locus_tag="ACHKIR_005148"
     CDS             2378..>2945
                     /locus_tag="ACHKIR_005148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002445110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992737.1"
                     /translation="MITIEKTKYPMSDEQVRRKLFWVLQRLSSYSLWQRKRDAWAYFT
                     QQYEQALKNWPEEITKGFDPEAIIWAYDALRLYDEGLSELAVGNRQVWQRLTGEFHQL
                     ARPVDLIESYFYLPCHERGVQQEPYPVEVEKLNKLKIAAKIHGDYLMYPPHNNVCNFF
                     DAAYLLELDNYKYNFNKLAYPVFPENLPP"
BASE COUNT          782 a          590 c          905 g          668 t
ORIGIN      
        1 ctggaaaatg ccgcagaacg ggcacaggcg ctgaaggctg atattgacag tcagatacag
       61 atgtttgagc agcggctgaa accgctcaat gaagccgtgc ttttctcggc gccggaaggc
      121 attgccctga ccagcggtga agatgtgcag ctggctgctg gtcagaacct ggctgtcaac
      181 gccggaggtg atatcagtac cggaacaatg ggcaacctga ccgcgctggc cggagagagg
      241 ctggggcttt ttgccaggag cggacaactg agcgtcaaat ccggggaggg gccagtagag
      301 gtacaggcgc aaaacggcag catgcggttg tttgctgaga agaagctgac gctgagttcg
      361 gcaagtgata tctcctttgc cgggaaaaaa cgtatcacgc tgattggcgg ggggagttac
      421 ctgcggctgg aggcggggaa aatagagtac ggtacgacgg tgtcgtatct gcgcaaagtt
      481 aaacggacta tagcggcggg ggctgcgtca cagcagccgg aaatgccttg gcttgccgac
      541 ccggttaaac cctctgccag tgcgctgatg cagtatctgt atcacgacga tgtcccggta
      601 gtgggcgcgc ctttcaaagc gactcttgct gatggcactg tacgggaagg taaccttgac
      661 ggtgcgggat atctgcatct ggaagacgtc ccgccaggga gagtgctggt ggagctcggg
      721 ccggatacca gagattattt acgtaaaaat aagaccgata atcggttgtt tgttgggaca
      781 tctctcagcg aggctgacat tgactcactg atagcgaagt acagcgaagg tgtggaatga
      841 gtggagtaaa cgttgcctgg tttaaggaaa ccagcctgga aaccggacag tggatctggg
      901 ggacgcttca gggggcattt aatgaaaaac agaccattgg gcagatcatc gttgatgcgg
      961 tgatcggcat gatcccgctg gtcggcgatg taacggcagt gcgagatctg atagcggttt
     1021 cgataggact ggcaaaagaa ccgaagaaac gggaaagtgt gactgaatgg gtgtttctgg
     1081 tgatcctgct gtttgcgcta atcccggtaa ttggtggcgt gataaaaggt gtcggacgtc
     1141 tggccctgcg tgtgacaaca aaagtggcag agaatgtgga aacccttggc gctgtgatta
     1201 agtttctgaa ccgtatcggc gaaggtgatg cggtgaagtg gctgaataag ctgaatgtcg
     1261 ttgagtatca ggcacagttg attgaaaaga tgggggattt ttgcaacacg atgattaccg
     1321 ccatcaataa aatcctgaaa gcccgggtcg ggcggatgtt gccggagaaa tggcaggcgg
     1381 atttactctt ggttcgggat ggttttgcgg cactgcaacg gctggcgtcg agaatgatcc
     1441 cgcaggcgtt gaaagagctg gatgcgaaac tgcgggtgtt gcaggcgctg gtttataaag
     1501 gtgaaataca tacaatcacg acggggatgg gacgtgctga agtgcgccgt gaggctgaag
     1561 catatcttat tgagcgtccg ctgtgtgaag cgccgaagca ggggacgcgg tatccgagtg
     1621 ttcatgcgga ggtaattcac gaagacagat tgcggcttaa atattccagg gagggatatc
     1681 cagatttgtt gggatataaa ggaaaaagtc cggcatttgg tgatgagcaa gtatttacaa
     1741 atattgcctc ttttagcggc gagattaccg caaaaagtgc aagtgagatg ggcggtagta
     1801 cgatttttcg cgcttttggt aatccaacag aattagcacc caacggttct tgggcggctg
     1861 gcggattctg ggggtatcaa aaagtgccgg gcagtgctga agaatggcgt cagctttcag
     1921 ctgtactgga tgagtggaat ggtaacggtt ttctggtggt gctgcatttg ccagaagacc
     1981 tggcaactcg tatgccgggt agcaatgctt ggcacgggaa aattgctgaa cagttcggac
     2041 agaagagacc tgaccagtat ctgcaaggtg gtggcgaaca ggtaattatt aatctggatg
     2101 aaacgttgaa aacagagttg aaccgcatcg gggagcaggt aaaagccagc ggtcaggctg
     2161 tcagtattga aattaatggc gtaaatgccg agttttatcc gacgggctgg aaagaggtgg
     2221 aaggcgtgca tggttacagt aagccggaag ggatagaacc gccgtatgtg acgtataccc
     2281 gtgaactgca tgacgatgaa ctgcgcagca aagtgacaga gcgaacgata cttaatgcgg
     2341 cccgcattga gtaccagacc acgacaggaa gcgaacaatg attaccattg aaaaaacaaa
     2401 atacccgatg agcgatgagc aggtacgtcg caaattattc tgggtgttac agcggctgag
     2461 cagctatagc ctttggcagc gcaaacggga tgcctgggcc tattttaccc agcagtatga
     2521 acaggcgctg aaaaactggc cggaagagat aacgaagggg tttgatcccg aggcgataat
     2581 ctgggcatat gatgctttgc gtctttacga tgaagggcta tcagaattgg cagtgggaaa
     2641 tcggcaggta tggcagcgtt taacgggaga atttcatcaa cttgctcgcc cagtggattt
     2701 aattgaatct tatttttatc tcccctgcca tgaacgaggt gtacagcaag aaccataccc
     2761 ggtagaagta gaaaagctta ataagctgaa gattgcagca aaaatacatg gtgactatct
     2821 aatgtatcca ccacataaca atgtatgcaa ctttttcgat gctgcatatt tattagaatt
     2881 agataattat aaatataatt ttaataagtt agcgtatccg gttttcccag aaaatctccc
     2941 ccctg
//
LOCUS       DAZGQL010000232         2937 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.234, whole genome shotgun sequence.
ACCESSION   DAZGQL010000232 DAZGQL010000000
VERSION     DAZGQL010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2937
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.234"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..911
                     /locus_tag="ACHKIR_005149"
     CDS             <1..911
                     /locus_tag="ACHKIR_005149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992738.1"
                     /translation="LCSEERYTIQINNAYNTIQSEKDDIESRIEKLENSVVELNKKLS
                     VQIPSEGEKKRRDEQFAAFYDYCRKVMSRNLAECFRIQKNRERSANSEILDNFLKGTH
                     LASDCFIAHSFGGFHEAIKHDVERSPGILCSIPVSIEIDTNTITGELVTAGVGVFRHE
                     GNDAKGSFTKIGPFFLKVDFSDQEWECNRPSFVVSGDDGKITISENGKVTPPSHQHCE
                     TLIEFAIDYLKNNKKQGLMKRIGRCMGYLQVAAEIEALVSGADKDAVVRETVLRDFNT
                     PPFKTEPDDWIQPGLTYLKGRFGAVI"
     gene            complement(904..1224)
                     /locus_tag="ACHKIR_005150"
     CDS             complement(904..1224)
                     /locus_tag="ACHKIR_005150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992739.1"
                     /translation="MNNNDNNLRKEFLQIMNENVKSELKALIPDNSETTQAILSEPYG
                     MLSTETLDIIITTLTPLMLQHLKHNINKWFNDELSHPGCSWDKNFACLQKKRLFNKLS
                     LKFR"
     gene            1350..1883
                     /locus_tag="ACHKIR_005151"
     CDS             1350..1883
                     /locus_tag="ACHKIR_005151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO9992740.1"
                     /translation="MNAKIKYGLSAAVLALIAAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGAIMVDGKPVIPGMKLSKEKCDRVNAIERDKALAWVEKNIRVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     ISRRDQESALACWGIDR"
     gene            2182..2649
                     /locus_tag="ACHKIR_005152"
     CDS             2182..2649
                     /locus_tag="ACHKIR_005152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFO9992741.1"
                     /translation="MNRVLCVVIIALLVACGALSLGLNHYRDNAITYKAQRDKKAREL
                     ELANATITDMQVRQRDVAALDAKYSRELADARAENETLRADVAAGRKRLRINATCHGT
                     VREATGTSGVDNATGPRLADTAERDYFILRERLMTMQKQLEGAQEYIRTQCIN"
     gene            2673..2897
                     /locus_tag="ACHKIR_005153"
     CDS             2673..2897
                     /locus_tag="ACHKIR_005153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992742.1"
                     /translation="MKKLLVTVKPFQGTIPFRILQRGRVLVEGSFSGKCTQLYSRTFQ
                     VNATNEELTVECTMNAAKCRMVSAALQPVC"
BASE COUNT          783 a          618 c          776 g          760 t
ORIGIN      
        1 gcctctgttc agaagagcgg tataccatac aaattaacaa tgcatataac acgatacagt
       61 cagaaaaaga tgatatcgaa tcacgcattg aaaaactgga gaacagcgtc gttgaactga
      121 ataaaaaact gtcggtgcag atcccttccg aaggtgaaaa aaaacgccgt gatgagcagt
      181 tcgcggcgtt ttacgattat tgccggaaag tgatgagcag aaatctcgca gagtgtttcc
      241 gtattcaaaa aaacagagag cgctctgcaa atagtgagat tctggataat tttttaaagg
      301 ggacgcatct cgccagtgat tgtttcattg ctcattcttt tggtggcttt catgaggcca
      361 tcaaacatga tgttgagaga agtccaggta tcctctgttc cattcccgta agcatcgaaa
      421 tagacacaaa tacgataacc ggtgaactcg ttaccgccgg agtcggtgta ttccgtcatg
      481 aggggaacga tgccaaaggt tcctttacaa aaatcgggcc gttctttctg aaggtagatt
      541 tcagtgacca ggaatgggag tgtaaccggc catcctttgt tgtatccggt gatgacggga
      601 aaataactat ctcagaaaat ggaaaagtaa caccgccatc gcaccagcac tgtgagacgc
      661 tcattgaatt tgccattgat tacctgaaga acaataaaaa gcagggacta atgaagcgca
      721 ttggtcgttg catggggtat ctgcaggtag ccgctgagat tgaagcgctg gtcagtggtg
      781 ctgataagga tgcagttgtg cgggagaccg ttcttcgtga tttcaatact ccacccttta
      841 agactgagcc agatgattgg atccagccgg ggctgactta tctgaaaggg cggtttgggg
      901 cggttatctg aacttaagcg atagtttatt gaagagccgt tttttttgca gacaggcaaa
      961 gtttttatcc catgaacaac ccgggtgact gagttcgtca ttaaaccatt tgttaatgtt
     1021 gtgcttcaga tgctgaagca tcaatggtgt taatgtggtg ataatgatat ccagtgtttc
     1081 tgttgaaagc atgccgtaag gctctgagag aatggcctga gtggtttcgc tattatccgg
     1141 gataagcgct tttaattctg atttaacatt ttcgttcatt atctgcaaaa actctttgcg
     1201 taaattgttg tcattattat tcatttataa aatctcattt gttgttaccg ggtgtgtcct
     1261 ggtgcctgtt gattatattc atcgtgagta ccggattata tatctgacat atccaggaat
     1321 aagagagcta taaatcctga taaatatcca tgaacgcaaa aatcaaatac ggcctgtcag
     1381 ctgccgttct ggcgctgatt gccgcagggg cttctgcgcc tgaaatcctc gaccagtttc
     1441 tggatgaaaa ggaaggcaac cacaccacgg cataccgtga tggtgcgggt atctggacca
     1501 tctgccgggg tgccatcatg gtggatggta agcctgtgat tcctggcatg aagctgtcga
     1561 aggaaaaatg cgaccgggtt aacgctatcg aacgggataa ggcgctggca tgggtggaga
     1621 aaaacatcag agtgccactg accgaacccc agaaagcggg tattgcgtca ttctgcccgt
     1681 ataacattgg ccccggtaag tgtttcccgt cgacgtttta taaacgaatt aatgcaggcg
     1741 atcgcagggg agcgtgtgaa gcgattcgct ggtggattaa ggacggtggc agagactgcc
     1801 gtatccgctc aaacaactgt tacggccaga tatcccgtcg tgaccaggag agcgcgctgg
     1861 cgtgctgggg aatcgacaga taagcagaat attttgctga aaaatgacgt tggccaacgc
     1921 ggacggataa cacgaaatcc tgcgaactgg caaaacctaa gtgaataaaa gtaaaaaccc
     1981 cgtttgttgg tagcaagcgg ggttttgtgt ttcctgactc cggaaaagtc aaaggagaaa
     2041 gtgtgtttaa ttttagcaaa ctgattcggg agattcgagt gatggctgaa aaattatcca
     2101 cctggaagtt catccttatc tggctggtgt ttgtgattat ggcctccggt tatttcatcg
     2161 gtcagatacg ctggtggtga aatgaaccgc gttctgtgtg tggtcatcat tgccctgctg
     2221 gtggcctgtg gtgcgcttag tctggggctg aatcattacc gcgataacgc catcacctac
     2281 aaagcgcagc gcgataaaaa agccagagag ctggagctgg caaacgcaac cattactgat
     2341 atgcaggtgc gccagcgcga tgttgctgcg ctcgatgcaa aatactcgag ggaattagcc
     2401 gatgcgagag ctgaaaatga aactctgcgt gctgatgttg ccgctggtcg taagcgcctg
     2461 cggatcaacg ccacctgcca cggtaccgtg cgtgaagcca ccggcacctc cggcgtggat
     2521 aatgcaaccg gcccccgact ggcagacacc gctgaacggg attatttcat cctcagagaa
     2581 cggttgatga caatgcagaa gcagctggaa ggggcgcagg aatatatccg cactcagtgt
     2641 attaactagt atttttgtta tccggagaat gcatgaagaa attactggta accgtaaagc
     2701 cttttcaggg aacaattccg ttccgtattt tgcagcgtgg tcgtgttctt gttgaaggtt
     2761 cgttcagtgg taaatgtacg caattatact cccggacctt tcaggtgaat gccacgaatg
     2821 aagagctaac cgttgagtgt acgatgaatg ccgctaaatg ccgcatggta tcggctgcat
     2881 tacagccagt gtgttgagcg accttattat ccatggcgcg gtattgtcgc cgtattc
//
LOCUS       DAZGQL010000233         2857 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.235, whole genome shotgun sequence.
ACCESSION   DAZGQL010000233 DAZGQL010000000
VERSION     DAZGQL010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2857
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.235"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            25..234
                     /locus_tag="ACHKIR_005154"
     CDS             25..234
                     /locus_tag="ACHKIR_005154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO9992743.1"
                     /translation="MSEDIPLPKINQRYRDDHGALVTVTSVEETRVVFMRDGYPHPCM
                     RPMYNFLGKFKPEPRKEPPAGNHTA"
     gene            complement(254..487)
                     /locus_tag="ACHKIR_005155"
     CDS             complement(254..487)
                     /locus_tag="ACHKIR_005155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992744.1"
                     /translation="MARSDYDIINLSPEHELNEWLAERGYAGQADNRNRVAEIVTRKL
                     QDSFYINVSWDALNTAYSEHPEWFSGLVSGDEN"
     gene            complement(576..1199)
                     /locus_tag="ACHKIR_005156"
     CDS             complement(576..1199)
                     /locus_tag="ACHKIR_005156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO9992745.1"
                     /translation="MRTRIDYLADKYSFTELNELPRLHQQWQDVLEECRQTEAGPEER
                     LRIALLNVDYVTSFELPFRLLLTRTPQLIAALREEWGISQKNVVFNDKRFGCVYSLKA
                     SLSGVPDTYRYHLSHRIRRVIGNENTSSPYQQIAREVKAPRERLKHALEAGLLVTALD
                     GLFWFGSQRIAADVLRLRKSGMPVVTTTAEVHDNLTGTTRKVPAYHL"
     gene            complement(1288..1797)
                     /locus_tag="ACHKIR_005157"
     CDS             complement(1288..1797)
                     /locus_tag="ACHKIR_005157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992746.1"
                     /translation="MQITEALISEPGEIRRFVQQAVDHWPNLLAFHFTLYSAEGINGQ
                     QIQTFCSSFHRRVHERITEHNHTVSPSAPVVLRWLREQHEGAQIRCLLLLSQTSICHP
                     RVGVMADEECAQLVDLLQQTWPVISAGGQCRVERCFRVARPGSSGQYVALKTAVQSLM
                     PRGIATIIR"
     gene            2255..2713
                     /locus_tag="ACHKIR_005158"
     CDS             2255..2713
                     /locus_tag="ACHKIR_005158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992747.1"
                     /translation="MKIYWTRKRIPELSALPPSLRKKNFTDAYNAASSHIEYWIGAGV
                     SFISMMILSRVYDFLLPTQDTFPGDIIRSLCVVCPSILIWFQFSVYVMRKYYRHILLR
                     GKETETISERLIREADTREYELWRPVRRFFSIIFLIVLLVCIHSLITTIK"
BASE COUNT          823 a          700 c          583 g          751 t
ORIGIN      
        1 tgaacggtta ccgttctgaa gtctatgtca gaagatattc cattaccaaa aatcaatcag
       61 cgatacagag acgatcatgg cgcgcttgtc accgtgacca gtgttgagga aacccgggtg
      121 gtgtttatgc gggacggcta tccgcatccc tgcatgcggc cgatgtacaa cttcctggga
      181 aaattcaaac ccgaaccccg gaaagaaccc cctgccggca atcacactgc ctgatggcag
      241 cataatccac ttattaattc tcatccccgg agacaagccc agaaaaccac tccgggtgtt
      301 cactgtatgc cgtgttcagc gcatcccaag aaacgttgat ataaaagctg tcctgcaatt
      361 tgcgggtaac aatttctgcc acccggttac ggttatccgc ctgtccggca taacctctct
      421 ctgccagcca ctcattcagt tcatgttccg gagacagatt gataatatcg taatcggacc
      481 ttgccattcc cgtttcctta gcacatgttt tcaggctcgg ggataaagaa tgtcgtactg
      541 ctgctgccag agcaatattc cccctgatgg tcgaatcaga gatggtaggc cggtactttg
      601 cgggttgttc ccgtcagatt atcatgcacc tccgcggtcg ttgtcaccac cggcattccg
      661 ctctttctca gcctcaggac atcagccgca atacgctgac taccgaacca gaacaggccg
      721 tccagtgcag tcaccagcaa ccccgcctcc agcgcatgct tcagccgttc acggggcgct
      781 ttcacttccc gggcaatctg ctggtatggc gatgatgtgt tttcattccc aatcacccgg
      841 cgaatacgat gagacagatg ataccggtat gtatccggta caccggaaag gctggccttc
      901 aggctgtaca cgcagccaaa tcgtttatca ttgaacacca catttttctg gctgatgccc
      961 cattcttcac gcagcgcggc aatcagttgt ggtgtccggg taagcaacaa gcgaaaaggc
     1021 agttcaaaac tggtgacgta atccacattc agcagtgcaa tacgcagccg ttcttccggc
     1081 ccggcctctg tctgccggca ctcctccaga acatcctgcc actgctggtg aaggcgtgga
     1141 agttcattca gttcagtaaa actgtattta tccgcaagat agtcaattcg tgtacgcata
     1201 ctgaagagta ttccgtataa agattcagct ggcaaaactt tatcagtctg taaaaactaa
     1261 cggaagagtc gatatttctc ccgtccatca ccggatgatt gttgcaatac cccgtggcat
     1321 cagagactga acagccgttt ttaacgccac gtattgccct gatgaaccag gccgggcaac
     1381 ccgaaaacat ctttccaccc ggcattgtcc gccagcactt atcaccggcc aggtctgttg
     1441 cagtaaatcc accagttgcg cacattcttc atcagccatg acgccgactc gcggatggca
     1501 aatactcgtc tggctgagca gcaacagaca tcgtatctgt gctccttcat gttgttcccg
     1561 caaccagcgt aataccaccg gcgctgatgg gctgaccgtg tgattatgct ccgtaatacg
     1621 ttcgtgtacc cgtcggtgaa aggaactaca aaatgtctga atctgttgac cattgattcc
     1681 ttctgccgaa tagagcgtga agtgaaatgc caacagattt ggccagtggt ccaccgcctg
     1741 ctgaacaaaa cgccggattt cccccggctc tgaaattaag gcttcggtta tttgcacgaa
     1801 atgttccttt tcaacccctg ttgaattttg tcaagccggt gcaggcacag taaagcgcgc
     1861 atcaacacct gaaaggtacg atacaaacac aagttacaaa caataagaac ggaaggacaa
     1921 ttaatcagtg aggaataata actaaattaa ccaaaagaat gttactgaca atactaccaa
     1981 tacttaaata gcaatatatt aattaatgta agaacagatg cccggtaaga catatacaca
     2041 atacatccac aaacacactc ttttcattaa atcaatcacc tgatcaaacc atgaaagtgt
     2101 gatgtaagtc aaaattactg acactatcaa ttgataatga ttatctctat cattatcgtg
     2161 aaattcgaga atgaaaggag aagtagtcat tataagcctt ttataaatcg tcatcatcat
     2221 agtaaagtag aacacttttt atatgagtta ctatatgaaa atatactgga caagaaaacg
     2281 cattcctgaa ctttcagcac ttccaccttc attaagaaag aaaaatttta ctgacgcata
     2341 taatgcagca tcttctcata tcgaatactg gataggtgct ggcgtctcat ttatctcgat
     2401 gatgatttta tccagagtat atgactttct tttacccacc caagatactt tcccgggtga
     2461 tatcatcaga agtttatgtg ttgtgtgtcc atccatatta atctggtttc agttttcggt
     2521 atatgtcatg aggaaatatt acagacatat tctgctgcga ggaaaagaaa ccgaaaccat
     2581 ttcagaaagg ttgatacgtg aggctgatac acgagaatat gaactatggc gtccggtccg
     2641 tcgctttttc tccattattt ttctcattgt tctattagtt tgcattcatt cactgataac
     2701 aactataaaa taaaagttat ggctgttctg atacaggaaa ataaacgttc attctatggt
     2761 tacctcttcc tgttatatag aagaatttta aacgacaagt aagggaatat aaatggaaat
     2821 aacaaaaaat aaaaatgcaa ctataaaaaa acagtca
//
LOCUS       DAZGQL010000234         2768 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.236, whole genome shotgun sequence.
ACCESSION   DAZGQL010000234 DAZGQL010000000
VERSION     DAZGQL010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2768
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.236"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..714
                     /locus_tag="ACHKIR_005159"
     CDS             <1..714
                     /locus_tag="ACHKIR_005159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFO9992748.1"
                     /translation="PKPTSLIKTLFGSVKTEDKDIYLDFFAGSGTTAHGILELNIEDG
                     RKRNFICVQLDEETDKKKQAYKEGYKSIFNITKDRIIKAGKKLKKDNPNYNGDLGFKI
                     FETVHDFRAKDESELTLSNLSFFDDAVLTPEQYDTLLTTWCVYDGSLLTTPIEDIDLN
                     GYKAHLCDGRLYLIAPNFTSEALKALLQKLDADKDFAPNKVVFYGSNFESAKQMELNE
                     ALKSYANKKSIDLDLVVRN"
     gene            717..>2768
                     /locus_tag="ACHKIR_005160"
     CDS             717..>2768
                     /locus_tag="ACHKIR_005160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015974229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III restriction-modification system
                     endonuclease"
                     /protein_id="HFO9992749.1"
                     /translation="MSKGFTFEKNLPHQKAGVDSVMNVFVSAIPHQTDHVAIRLLANP
                     ELNLSEQQYYNNIKNVQEFNGIEHSKDNYDAKSNVIDVSMETGTGKTYTYTKTIFDLN
                     KSFGINKFIIIVPTLSIKAGTVNFLKSDAIKEHFRDDYERELKTYVVESQKSSGKNTK
                     SYMPQAIHDFVEASNFNKKYIHILVINSGMINSKSLTDTYDVGLLDNQFDTPFSALSA
                     IKPFIIIDEPHKFPTGKKTWEHIQKFNAQYIIRYGATFSEGYKNLVYRLTAVDAFNED
                     LVKGIDAYIEDIVGDGNANLKLVKSDGKEATFELNENNNKKIFKLAKGESLSKTHSAI
                     HDLTLDALNKNTAVLSNGIELKIGSSINPYSYDQTLADNMMRKAVKEHFKLEKEYLTQ
                     RPRIKPLTLFFIDDIEGYRDGNDIAGSLKTKFEEYVLAEANELLKTEKDEFYRNYLEK
                     TVKDISSVHGGYFSKDNSDKDDKIEQEINEILHDKELLLSLENPRRFIFSKWTLREGW
                     DNPNVFQICKLRSSGSTTSKLQEVGRGLRLPVNEYMCRVKNRDFTLNYYVDFTEKDFV
                     DSLVKEVNDSSFKETVPGKFTQELKDKILSQYPELSSRTLMNEIFDAEIIDDNENFKD
                     SDAYSRLKAKYPAAFPAGVKPSKIKKATDGKKRTKMRIGKFSELKELWDLINQKVVIE
                     YK"
BASE COUNT          980 a          460 c          522 g          806 t
ORIGIN      
        1 ccgaagccaa caagtttaat aaaaacacta tttggttcag taaaaaccga agataaagac
       61 atttatcttg acttttttgc tggctctgga actacagctc atggtattct ggagttaaat
      121 attgaagacg gcaggaaaag aaacttcatc tgtgttcaat tagatgaaga aacagataaa
      181 aagaaacaag cctataaaga agggtataaa tccatcttca atataacaaa agacagaatt
      241 attaaggcag gaaaaaagct gaaaaaagat aatcctaatt ataatggaga tttgggattt
      301 aaaatatttg aaacagttca tgattttcga gcgaaagatg agtctgaact tacgctttct
      361 aatcttagtt tctttgacga tgctgtattg acaccagagc agtatgacac tctattgacg
      421 acatggtgcg tgtatgatgg cagcttgcta acaacaccaa ttgaagatat tgaccttaat
      481 ggctacaaag cacacttgtg cgatggtcgg ctatatctaa ttgcgcctaa tttcacaagt
      541 gaggctttga aagctttact tcaaaagctg gatgcagaca aagactttgc ccctaacaaa
      601 gttgtatttt atggcagcaa cttcgaaagt gcgaaacaga tggaactcaa tgaagcacta
      661 aaaagttatg ctaataaaaa atctattgat ttagatttgg tggtaaggaa ctaatcatgt
      721 ctaaagggtt cacattcgaa aaaaatttac ctcatcaaaa ggctggcgtt gattcggtaa
      781 tgaatgtctt tgtatccgcc atacctcatc agacggatca tgttgcaatt cgcctgttag
      841 ctaatccaga actgaatcta tctgaacaac aatactacaa caacataaaa aatgttcagg
      901 agttcaatgg tattgagcac tcaaaagata attacgatgc taaaagcaac gtaattgatg
      961 tttctatgga gacaggtaca ggtaaaactt atacttacac caaaacaatt tttgacctga
     1021 acaaatcatt tggtattaac aaatttatca tcattgttcc taccctatcg atcaaagccg
     1081 gaaccgttaa ctttttaaaa agcgatgcta taaaagaaca ctttcgagat gattacgagc
     1141 gtgaactaaa gacttacgtt gttgaaagtc agaaaagttc gggtaaaaat acaaaatcgt
     1201 atatgcctca agctatccat gattttgttg aagcaagtaa tttcaataag aaatatatac
     1261 acattcttgt gatcaactca gggatgatta actctaagtc tttaactgac acttatgatg
     1321 ttggcttgct tgataatcag tttgataccc cattttctgc acttagtgcg ataaaaccat
     1381 tcattatcat tgatgagcct cataaatttc ctactggtaa aaaaacatgg gaacatattc
     1441 aaaaatttaa tgcccaatat atcatccgct atggcgcaac ctttagtgaa ggctataaga
     1501 acttagttta tcgtcttaca gccgtagatg ccttcaatga agatcttgtt aaaggtattg
     1561 atgcatacat tgaggatatt gttggtgatg gtaatgccaa cctcaagttg gtcaaatctg
     1621 acggaaaaga agctacattt gaactgaacg aaaataataa taaaaaaatt tttaagttag
     1681 ccaaaggtga atcactatct aagacgcata gtgcgatcca tgatttaact cttgatgctt
     1741 taaataagaa tacagcagtg ttgagtaatg gtattgagct aaaaattggt agctcgataa
     1801 acccctattc ttacgatcag acacttgctg ataacatgat gcgtaaagct gtcaaagaac
     1861 actttaagtt agaaaaagag tatctgacac aaaggccacg cataaaacct cttactcttt
     1921 tcttcattga tgacattgaa ggctatcgtg atggtaatga tattgcagga agcctgaaaa
     1981 ctaagttcga agaatacgtt ttggcagaag caaatgaact gttaaaaaca gagaaagatg
     2041 agttttacag aaactacctt gaaaaaactg ttaaggatat atcttctgtt catggtggtt
     2101 atttttcaaa agataatagt gataaagatg ataaaattga gcaggaaatc aacgaaatcc
     2161 ttcatgataa agagcttcta ttgtctttag aaaatcctcg ccgtttcatt ttctctaaat
     2221 ggacgttgcg tgagggatgg gataacccaa atgtctttca gatttgtaag ctccgttcga
     2281 gcggtagcac cacatctaaa cttcaggagg tagggcgtgg tttacgtctt ccagtgaatg
     2341 aatacatgtg ccgtgtgaaa aaccgcgatt ttactctcaa ctattatgtt gattttacgg
     2401 aaaaagattt tgttgattct cttgtaaaag aggttaatga tagttctttc aaagaaactg
     2461 ttccaggtaa gtttactcaa gaacttaagg ataaaatctt atctcagtat cctgagcttt
     2521 catccagaac actcatgaat gagatctttg atgctgaaat tattgatgat aatgaaaact
     2581 tcaaagattc cgatgcctat agccgtttaa aagcaaaata tccggctgca ttccctgccg
     2641 gggtgaaacc aagcaaaata aaaaaagcca cggatggaaa aaagcgtact aaaatgcgta
     2701 ttggtaagtt cagtgagttg aaagaactat gggatttgat caatcaaaaa gtagtaattg
     2761 agtataaa
//
LOCUS       DAZGQL010000235         2692 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.237, whole genome shotgun sequence.
ACCESSION   DAZGQL010000235 DAZGQL010000000
VERSION     DAZGQL010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2692
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.237"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(37..>2692)
                     /gene="chiA"
                     /locus_tag="ACHKIR_005161"
     CDS             complement(37..>2692)
                     /gene="chiA"
                     /locus_tag="ACHKIR_005161"
                     /EC_number="3.2.1.14"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional chitinase/lysozyme"
                     /protein_id="HFO9992750.1"
                     /translation="CSALPALAMEVWNNQQGGNKYQVIFDGKIYENAWWVSSTNCPGK
                     AKANDATNPWRLKRTATAAEISQFGNTLSCEKSGSSSSSNSNTPASNTPANGGSATPA
                     QGTVPSNSSVVAWNKQQGGQTWYVVFNGAVYKNAWWVASSNCPGDAKGNDASNPWRYV
                     RAATATEISETSNPQSCTSAPQPAPDVKPAPDVKPAPDVQPAPADKSNDNYAVVAWKG
                     QEGSSTWYVIYNGGIYKNAWWVGAANCPGDAKENDASNPWRYVRAATATEISQYGNPG
                     SCSVKPDNNSGAVTPVDPTPETPVTPVTPTPDNNEPSTPADSSNDYSLQAWSGQEGSE
                     IYHVIFNGNVYQNAWWVGSEDCPRGTSAENSNNPWRLVRTATAAEMSQYGNPTTCEID
                     NGGVIVADGFQASKAYSANSIVDYNDAHYKTSVDQDAWGFVPGGDNPWKKYEPAKAWS
                     ASTVYVKGDRVVVDGQAYEALFWTQSDNPALVANQNATGSNSRPWKPLGKAQSYSNEE
                     LNNAPQFNPETLYASDTLIRFNGANYISQSKVQKVSPSDSNPWRVFVDWTGTKERVGT
                     PKKAWPKHVYAPYVDFTLNTIPDLAALAKNHNVNHFTLAFVVSKDANTCLPTWGTAYG
                     MQNYAQYSKIKALREAGGDVMLSIGGANNAPLAASCKNVDDLMQHYYDIVDNLNLRVL
                     DFDIEGTWVADQASIERRNLAVKKVQDKWKSEGKDIAIWYTLPILPTGLTPEGMNVLS
                     DAKAKGVELAGVNVMTMDYGNAICQSANTEGQNIHGKCATSAIANLHSQLKGLHPNKS
                     DAEIDAMMGTTPMVGVNDVQGEVFYLSDARLVMQDAQKRNLGMVGIWSIARDLPGGTN
                     LSPEFHGLTKEQAPKYAFSEIFAPFTKQ"
BASE COUNT          624 a          713 c          646 g          709 t
ORIGIN      
        1 aaaatgcggc gataccggaa ggtatcgcca acacatttat tgcttagtaa acggcgcgaa
       61 gatttcgcta aatgcgtact tcggtgcctg ttctttagtc aggccgtgga attccggaga
      121 caggttagtg ccgcctggca ggtcgcgcgc gattgaccag ataccaacca taccgagatt
      181 acgcttctgc gcatcctgca tgaccagacg agcatcagag agatagaaca cctcgccctg
      241 aacgtcgttc acgccaacca tcggcgtggt acccatcata gcgtcaattt ctgcatcgct
      301 cttattggga tggaggcctt tcaattgtga atgcaggttg gcaatcgcag acgttgcaca
      361 cttaccgtga atgttctggc cttcggtatt tgcagactga caaatcgcgt taccgtagtc
      421 cattgtcatc acgttcacac ccgccagctc aacacctttc gctttggcat cgctcaggac
      481 attcatccct tccggcgtca ggccagtcgg cagaattggc aaggtgtacc agatagcaat
      541 atctttgccc tctgacttcc atttatcctg cactttcttc acagcaaggt tacgacgttc
      601 aatagatgcc tgatccgcaa cccaggtgcc ttcgatatcg aagtccagga ctctgaggtt
      661 caggttatca acgatgtcat aataatgctg catcagatcg tctacgttct tacaggaagc
      721 agccagcgga gcgttgttag caccaccgat agacagcatc acatcgccgc cagcttcacg
      781 cagagctttg attttgctgt actgagcgta attctgcata ccgtaagcgg taccccatgt
      841 cggcagacag gtgttcgcat ctttactcac cacaaacgcc agcgtgaagt ggttgacgtt
      901 atgattctta gccagcgcag ccagatccgg gatcgtattc agcgtaaagt caacatacgg
      961 tgcataaacg tgtttcggcc acgctttctt cggcgtacct acacgttctt tggttccggt
     1021 ccagtcaaca aaaacacgcc acgggttgct gtcagaagga gaaactttct gcactttact
     1081 ctgagaaatg tagttcgcac cgttaaagcg aatcagcgta tcgctggcat aaagcgtttc
     1141 tggattaaac tgcggcgcat tattcagctc ttcgttgcta tagctctgag ccttacctaa
     1201 cggcttccac gggcggctat tgctaccggt ggcgttttgg ttcgcaacca gagctgggtt
     1261 gtcgctttgc gtccagaaca gcgcttcata agcctgccca tcaacaacaa cgcgatcacc
     1321 tttcacgtac acggtggaag cggaccatgc tttcgccggt tcgtatttct tccacgggtt
     1381 atcgccgccc gggacaaagc cccatgcgtc ttgatcgaca gaggttttat aatgtgcatc
     1441 gttataatct acgatgctgt tcgcgctgta cgctttgctg gcctggaaac catccgcaac
     1501 aatgacgccg ccattatcaa tttcacaggt agttgggtta ccgtactgac tcatttccgc
     1561 agcggtagct gtacgcacga gacgccatgg gttattggag ttttctgcac tagtgccacg
     1621 tgggcaatct tcagaaccaa cccaccaggc attctgataa acattaccat tgaagataac
     1681 atggtaaatt tcgctacctt cctggccact ccacgcctgc agtgagtaat cgttactgct
     1741 atccgctggt gttgatggct cgttattatc cggagtagga gtaactggag ttaccggtgt
     1801 ttccggagtt ggatcaaccg gagtcacagc accgctatta ttatccggct taacggaaca
     1861 ggaacccgga ttaccatact ggctgatttc tgttgccgtt gccgcgcgaa cataacgcca
     1921 tgggttgctg gcatcgtttt ctttcgcatc gcctgggcaa tttgccgcgc ctacccacca
     1981 ggcgttctta taaatgccgc cgttgtagat aacgtaccat gtagaagaac cttcctgacc
     2041 tttccaggct acgacagcat agttgtcgtt tgacttatca gctggagctg gctgaacatc
     2101 aggagccggt ttaacgtccg gtgccggttt cacatccggc gcaggctgtg gtgctgaagt
     2161 acaggactgt ggattactgg tttctgagat ttccgttgcc gtagcggcac gaacataacg
     2221 ccatgggttg ctggcatcat tgcctttcgc atcacccggg caattagaag aggctaccca
     2281 ccaggcattt ttatataccg caccattaaa gacgacatac caggtctgac cgccctgctg
     2341 tttattccag gcaactacag aagaattaga cggaacagtg ccctgtgctg gtgtagccga
     2401 accgccatta gccggcgtat tggatgcagg cgtatttgaa tttgaagaag atgagctgcc
     2461 gctcttttcg caggaaagtg tattgccaaa ctgactaatt tcagcagctg tcgcggtacg
     2521 ctttaaacgc catgggttag ttgcatcatt tgcttttgct tttcccgggc aatttgtaga
     2581 agaaacccac caggcatttt cataaatttt gccatcgaaa ataacctgat atttattacc
     2641 accttgttgg ttattccata cttccattgc caatgctggc agagcggaac ac
//
LOCUS       DAZGQL010000236         2591 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.238, whole genome shotgun sequence.
ACCESSION   DAZGQL010000236 DAZGQL010000000
VERSION     DAZGQL010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2591
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.238"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..370)
                     /locus_tag="ACHKIR_005162"
     CDS             complement(<1..370)
                     /locus_tag="ACHKIR_005162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFO9992751.1"
                     /translation="MSNDYPLVSIIIPTYNSSDYITETLTKLEKQTYPNFEIVIVNDG
                     SKDNTSNVLREYGLTHSRLIIINKENGGVSSARNTGIRKAQGQFICFMDDDDEIDPNY
                     LLKMYSRQHETGGDAIYCGLY"
     gene            complement(452..1480)
                     /locus_tag="ACHKIR_005163"
     CDS             complement(452..1480)
                     /locus_tag="ACHKIR_005163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001592907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactose--(galactosyl) LPS
                     alpha1,2-galactosyltransferase"
                     /protein_id="HFO9992752.1"
                     /translation="MDLLAESITEVAVSGEIANTDRVLNIAYGIDRNFLFGAAVSMQS
                     VVMHNPDLAVKFHLFTDYIDEDYLQRVNAFTSKNANVEVKIYKVSSAFIDIFPSLKQW
                     SYATFFRLVAFQYLSEVIENLLYIDADVICKGSLAGLLDINFDGDKFAAVIKDVPFMQ
                     EKPAKRLAIEGLPGNYFNAGVVYLQLEAWAKNDFMNKAIAMLASDPQHTKYKCLDQDI
                     LNILFFGHCIFISGDYDCFYGIDYELKNKSDEDYKKTITDDTKLIHYVGVTKPWNDWT
                     NYPCQKYFNEAYQASCWNDVAFIPATNEKQYQVKYQHAKKNGDTFNAFIYFIKFKLNK
                     YKRKLFGQ"
     gene            complement(1506..2198)
                     /gene="rfaY"
                     /locus_tag="ACHKIR_005164"
     CDS             complement(1506..2198)
                     /gene="rfaY"
                     /locus_tag="ACHKIR_005164"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HFO9992753.1"
                     /translation="MITSIRYRGFSFYYKDNDNKYKEIFDEILAYNFKTVKVLRNIDD
                     TKVSLIDTKYGRYVFKVFAPKTKRNERFFKSFVKGDYYQNLIVETDRVRSAGLTFPND
                     FYFLAERKIFNYASVFIMLIEYVEGVELNDMPIIPENVKAEIKASMEKLHALNMLSGD
                     PHRGNFIVSKDGVRIIDLSGKSCTAERKARDRLAMERHLGIANEIKDYGYYSVIYRTK
                     LRKFIKKLKGKA"
     gene            complement(2208..>2591)
                     /locus_tag="ACHKIR_005165"
     CDS             complement(2208..>2591)
                     /locus_tag="ACHKIR_005165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008918 - lipopolysaccharide
                     3-alpha-galactosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 C-terminal
                     domain-containing protein"
                     /protein_id="HFO9992754.1"
                     /translation="YGSLKYLDQDALNIAFNMNNIYLAKDFDTIYTLKNELHDRSHRK
                     YQQTITDKTVLIHYTGITKPWHSWAGYPSASYFNIAREQSPWKKYPLKEARTVAEMQK
                     QYKHLFAHGEYIKGITSLIKYKLKK"
BASE COUNT          803 a          505 c          429 g          854 t
ORIGIN      
        1 cataaagccc acaataaatc gcatcccctc ccgtctcatg ttgtctggaa tacatcttca
       61 gcagatagtt aggatctatc tcatcatcat catccataaa acatataaac tgtccttgcg
      121 ccttgcggat acctgtattc ctggcagacg aaacaccgcc attttcttta ttgataataa
      181 ttaatcgaga gtgggttaac ccatactctc tcaaaacatt tgatgtgtta tctttagaac
      241 catcattaac aataacaatt tcaaaatttg ggtaagtttg tttttctaat ttcgttagag
      301 tttcagtaat gtaatcagat gaattatacg tcggtattat tatggatact aaagggtaat
      361 cattgctcat cgtgttctct tgcctgaact aataaaatat gatagtactc atccttaatt
      421 attattgtaa ctcagacatc catgattttt attattgccc aaatagtttt cttttatact
      481 tatttaattt gaatttaatg aagtaaataa aagcgttaaa cgtatcacca tttttctttg
      541 catgttggta tttcacttga tactgctttt cattcgtggc tggaataaac gccacatcat
      601 tccagcaaga agcctgataa gcctcattaa aatacttctg gcagggataa ttcgtccagt
      661 cgttccaggg cttcgttacg ccaacataat gaatcagctt agtatcatcg gtaatggtct
      721 ttttataatc ttcatcgctt ttatttttta actcatagtc aatgccataa aagcaatcat
      781 aatcgccgct aataaaaata caatgaccaa agaacagaat atttaaaata tcctgatcaa
      841 ggcatttata tttcgtgtgc tgcgggtcac ttgccagcat agcaatggct ttattcataa
      901 aatcattttt cgcccatgct tcaagctgca gatatactac accggcgttg aaataattac
      961 ctggaagtcc ctctatagcc agacgcttcg ctggtttttc ctgcataaaa ggcacatctt
     1021 taataacagc tgcgaactta tcgccatcaa aattaatatc aagcaatcca gctaatgagc
     1081 ctttacagat gacatcagca tcgatatata acagattttc aataacttca ctcagatact
     1141 ggaacgcaac taaacggaag aatgttgcat aagaccactg tttcaggctg gggaagatat
     1201 caataaaggc actggagact ttataaattt ttacttcaac gttagcattt ttgctggtaa
     1261 aagcgttaac acgttgtaga taatcttcat caatgtagtc ggtaaagaga tgaaacttaa
     1321 ccgcaaggtc cgggttatgc ataacaactg attgcataga tactgccgca ccaaataaaa
     1381 aattacggtc aataccgtaa gcgatattta acacacgatc ggtgttagca atttccccag
     1441 agacagcgac ttcagtaata ctctcagcta ataaatccat gttggttccg ttttgactgt
     1501 gtggtttacg ctttgccttt taatttttta ataaatttgc gtagtttggt tctgtagatt
     1561 actgaatagt agccataatc ttttatttcg ttggcaatac ccaggtgacg ttccatggcc
     1621 agacgatcac gagctttacg ctctgccgta cagcttttac cggataggtc aattatcctg
     1681 acaccatctt tactaacgat aaagttaccc cggtgagggt cgcccgatag catatttaag
     1741 gcatgcaatt tttccattga agccttaatt tctgctttaa cattttctgg aataatcggc
     1801 atatcattga gttctacgcc ctcgacatac tcgataagca tgataaaaac actggcatag
     1861 ttgaaaattt tacgctcagc cagaaaataa aaatcattag gaaaagtaag acctgcactt
     1921 ctgacacgat cggtttcaac gatcaaattc tgatagtagt caccttttac aaaagattta
     1981 aaaaaacgtt cattcctttt cgttttggga gcaaagactt taaaaacata gcgaccatat
     2041 ttcgtatcaa tcaacgatac tttggtatca tcaatattac gtaatacttt tactgtttta
     2101 aaattatagg ccagtatttc atcaaagatt tctttatatt tattatcgtt atctttgtaa
     2161 taaaatgaga agccgcgata gcgtatactt gtaatcataa gagtaattta tttcttaagc
     2221 ttgtacttaa ttaatgaagt tataccttta atatactcac catgggcaaa cagatgctta
     2281 tattgtttct gcatttctgc aacagtccgc gcctctttaa gaggatattt cttccaggga
     2341 gattgttcac gcgcgatatt aaagtatgat gcagatggat atccagccca gctatgccat
     2401 ggtttagtta tccctgtata gtgaatcaat actgttttat cggtaatggt ttgctgatac
     2461 tttcgatgac tacgatcatg aagttcgttt ttcagggtat aaatagtatc aaaatccttc
     2521 gcgaggtaga tattattcat attaaatgcg atattcaacg catcctgatc taaatattta
     2581 agagagccat a
//
LOCUS       DAZGQL010000237         2580 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.239, whole genome shotgun sequence.
ACCESSION   DAZGQL010000237 DAZGQL010000000
VERSION     DAZGQL010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2580
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.239"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            94..188
                     /locus_tag="ACHKIR_005166"
     tRNA            94..188
                     /locus_tag="ACHKIR_005166"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:128..130,aa:Sec,seq:tca)
     gene            488..1693
                     /locus_tag="ACHKIR_005167"
     CDS             488..1693
                     /locus_tag="ACHKIR_005167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO9992755.1"
                     /translation="MALTDIKVKTAKPKEKPYKLADGGGMYLLINTNGSKYWRMKYRF
                     AGKEKMLSIGVYPDVTLADAREKRSEARKILAAGGDPGVAKKEEKIAQQMSLKNTFEA
                     IAREWHQSKADRWSLRYRDEIIDTFKKDIFPYIGKRPIAEIKPMELLEALRKMEKRGA
                     LEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLACALATPKKVHFPFLTANELPHFLND
                     LAGYTGSIITKTATQIIMLTGVRTQELRFARWEDIDFKTKLWEIPAEVMKMKRPHIVP
                     LSEQVIALFKQLEPITKHHPLVFIGRNDPRKPISKESINQVIELLGYKGRLTGHGFRH
                     TMSTILHEQGFNSAWIETQLAHVDKNAIRGTYNHALYLDGRREMMQWYADYIDSLSSH
                     ESQCQGPLY"
     gene            2101..2367
                     /locus_tag="ACHKIR_005168"
                     /pseudo
     CDS             2101..2367
                     /locus_tag="ACHKIR_005168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          770 a          568 c          540 g          702 t
ORIGIN      
        1 gcgtgtatta ggcggaaaaa actgatctgg gggatgtaga aactcaagga agtagctata
       61 atgcgccccg cctccatgta gcaatcgagg cgcggaagat cgtcgtctcc ggtgaggcgg
      121 ctggacttca aatccagttg gggccgccag cggtcccggg caggttcgac tcctgtgatc
      181 ttccgccaat taacatcttc tgaactctcc cgatgtcaat cccccccttc taatcccttg
      241 tatatcagga ttcctttgta tccagaagtc aaccgacttc accccgtatc aagttgcttt
      301 tgggggtact ttagggggta ttatttccta ttgaaatatc agcaccccca taattggtaa
      361 aatatgatat ataacaacgg ctttcaacaa catctttgtt atacaagggg tgcattgggg
      421 gtatcaggtt aattactggc agaaatcccc ctcaggaaca tgaattcatt aaaagccgga
      481 ggatgtcatg gcattaaccg atattaaagt gaaaaccgca aaaccaaaag aaaagcccta
      541 taaacttgct gatggcggcg gtatgtatct actgattaac actaatggtt ctaaatactg
      601 gcgtatgaag taccgctttg ctggtaaaga aaagatgctt tctattggcg tataccccga
      661 tgtaacgcta gccgacgctc gtgaaaaacg cagcgaagcc agaaaaatcc tcgctgctgg
      721 aggcgatcca ggggtggcaa aaaaagaaga aaaaatcgcg cagcaaatga gtttgaaaaa
      781 tacgttcgag gccattgctc gcgaatggca tcagtcaaaa gccgatcggt ggtcattacg
      841 ctatcgtgat gaaatcattg atacctttaa aaaagacatt tttccctaca ttggcaaacg
      901 ccctatcgcc gaaatcaaac caatggagtt actggaagca ctgcgtaaaa tggaaaaacg
      961 tggagctctg gagaaaatgc gcaaagtccg ccagcgctgt ggtgaggtgt tccgctatgc
     1021 aattgttact ggtcgggcag actacaaccc tgctcccgat cttgcctgtg ctttagctac
     1081 accgaaaaaa gtacattttc cctttcttac tgctaatgaa cttccccact tcctcaacga
     1141 tctggcgggt tataccggaa gtatcatcac taaaacagct actcagatca ttatgttaac
     1201 cggtgtacga acacaagaat tacgttttgc acgctgggag gatatcgatt ttaagacaaa
     1261 attatgggag attccggcag aagtaatgaa gatgaagcga cctcatattg tgccgctctc
     1321 tgagcaggtc attgccctat ttaagcaact tgaacccatc acaaaacatc atcctctggt
     1381 ctttatcggt aggaacgatc ctcgcaagcc aattagtaag gaaagcatta accaagtcat
     1441 tgaattactg ggatataagg gaagactcac agggcacggt ttcagacaca ccatgagtac
     1501 cattctacac gaacagggct ttaattccgc ttggattgaa acgcagttag ctcatgtaga
     1561 taaaaacgct atcaggggga cctataatca tgccctgtat ctagatggtc gccgtgaaat
     1621 gatgcaatgg tacgctgatt acattgattc gctttccagc cacgagagtc agtgtcaagg
     1681 ccccttgtac tgatattttc tctcaattca caaaattcct ccggcctcca ggtctggagg
     1741 aattttgttt tcatgtttta tttttagata ttttttgaca aaatatttat attgcatctg
     1801 caaacaatct cagaacatca caccgctata gaccgctaac aattttgatg cctgtaattt
     1861 caatcatcat cctgtcgtta tttcagcacc atcacaccaa aaatcaacta acatgttgtc
     1921 ttagccgact attcacaatg cattggaatg cagataaaga taaattttta tccacatgaa
     1981 gaattactgt cgatgatatt gtaaacaata ttcatcccgg gcaataacaa gacttgatac
     2041 acaacatcac gacttttatg cttttcagat tgtgtcccat ccgttttaca ggctcagaaa
     2101 atgaacgaca gattacgctt tgatgtgaac gataaccaag gccgtttcgt atttccgaat
     2161 accaggtttg gcccattact ggaggagttt gaagaagtgc atgacgcata cggcactgat
     2221 gaaatcagcg agacgagtta tatcaacaaa ctcaggtagc ttgcccaacg ggagcctgac
     2281 tttattgata tacacgctca tctggcatat gcatttctgg agcagccaag gtgggcaata
     2341 tttttattta tttctgtgaa aagcctgcta aagatcccat gcttctatat taatgactgg
     2401 aacaatacta ggaagcacat taagaaaaaa taaaggaagc cgatggctac cacgtttatc
     2461 gtttagaagc gtgagctctt cattattaag ccggacactt acaaaatgaa gttgcttctg
     2521 ttaaaatttc gttttgctac ggcttctata atagggactt ttactaaagt tatttttact
//
LOCUS       DAZGQL010000238         2573 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.240, whole genome shotgun sequence.
ACCESSION   DAZGQL010000238 DAZGQL010000000
VERSION     DAZGQL010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.240"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..816)
                     /locus_tag="ACHKIR_005169"
     CDS             complement(<1..816)
                     /locus_tag="ACHKIR_005169"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001149830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HFO9992756.1"
                     /translation="MQCEFKGVISALPTPYDQSQQIDMESLRKLIRFNIEQNIKGLYV
                     GGSTGEAFLQNVAEREKILETVADESDGRLTLIAHVGGISTAESEVLAKAAKKYGYHA
                     ISAVTPFYYPFSFEEHCIHYRKIIDSADGLPMVVYNIPALSGVRFSLDQINELVTIPG
                     VCALKQTSGDLFQMEQIKRNHPELVLYNGYDEIFASGLIAGADGGIGSTYNIMGWRYL
                     EIFEAVKNNDVIKAKEMQVACNQVIDTLIQSGVLAGIKTLLYYMGIINTPVCRS"
     gene            934..1486
                     /locus_tag="ACHKIR_005170"
                     /pseudo
     CDS             934..1486
                     /locus_tag="ACHKIR_005170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005154742.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1720..2406)
                     /locus_tag="ACHKIR_005171"
     CDS             complement(1720..2406)
                     /locus_tag="ACHKIR_005171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFO9992757.1"
                     /translation="MATMTDHQKEFISLFNRTACYHKRHKVFEDFVSCSVIALENRLQ
                     FSEAREQKYLRIVSGYEKQDVIHIAHLLAHVIEGLEQGFCDFLGSVFMQLELGDKYRS
                     QFFTPWSIASMMAQMQMGDVKALFENKPFITLSEPACGAGSMILAMADILNRSGYPAY
                     HRMWVSATDIDPLAAGMAYIQLSLCGVAGEVVIGNSLCNERRRVLLTPGHYLGNWDHR
                     LRHLQEQNVA"
BASE COUNT          745 a          654 c          510 g          664 t
ORIGIN      
        1 acttctgcat acaggagtat ttataatccc catatagtaa agaagagttt ttatacctgc
       61 caggacacca ctctgaataa gagtatcaat aacctggtta caggccacct gcatttcctt
      121 cgccttaatc acatcattat ttttgactgc ttcaaatatt tcaagataac gccaccccat
      181 tatattatat gtactaccga ttcccccatc tgcacctgca attaaccctg atgcaaatat
      241 ttcatcgtaa ccattatata acacaagctc tggatgattc cttttgattt gttccatctg
      301 gaataaatca ccggatgtct gttttaatgc acaaaccccg ggtattgtga caagctcatt
      361 tatctgatct aaagaaaaac gcactccgct aagagccggt atattataaa ctaccatcgg
      421 aagtccatct gcagaatcaa taatcttcct gtaatggata caatgctctt caaatgaaaa
      481 aggataataa aatggagtaa cagcagatat agcatgatat ccgtattttt ttgcagcttt
      541 tgcaagtacc tcgctttctg ccgtactgat tccgcccaca tgcgctataa gtgttagtct
      601 tccatctgat tcatcggcta ctgtttcaag aattttttct cgctctgcaa cattctgaag
      661 aaatgcttct ccagtagagc ctccaacata caatccctta atattttgtt cgatattaaa
      721 acggatcagt tttcgaagac tctccatatc gatttgctgg gattggtcat aaggagtggg
      781 taatgctgat ataacccctt taaattcaca ttgcattttt aatcaccttc acttaactgc
      841 ataatgggat acctagtaca ccatcaagta caaagattat ccaacagaaa agaagcacaa
      901 tgtgaagatt atcaaaaaaa agccatcaga aagctgtcat gccgctcagt taaggatcgg
      961 ttgatcgatc cagtgtccag ttactattct ctgcgtaacc ctctgcgact atcttgccct
     1021 ggaactgatt acccacagcc ctgaagagtc cgacactgtc acgctacaca aacggcgtct
     1081 tcctctggaa atgatggtct ggtgaattat cagtatggcc catgagcgta aggaaccttc
     1141 atcagattgt gaaccggctg gacatcatgc tgccaggcag tagcgccgtg ctccatgccc
     1201 gccagcggcc aggaagcgga gccatacgct gcgtgttcac tcaaacagcg cagctctagc
     1261 ataacaccac gccatatccg ctctggtgcg atctgaatct gctgaccatt gatggcgtgt
     1321 tctgacgaac acaggacacg caagaacacg ataaagcttc actcgccaaa tacatagtgg
     1381 aaaccagaca ctttatcctc aggtaaaaat ggtctgtctg atagaactga ccagccatct
     1441 actgacggca gcggccttcg atacaatgaa tctggcacta aataagactg gcaatatagc
     1501 cgataatgcg ttgaatcagt ggctatatcg tatacataaa acagacatca ttattctgtc
     1561 ttgttgtcca acacataaaa actaataaaa acataccatt ccctgtcgtc agagaattta
     1621 ttgataaaca ggctttattg aacaaatcaa acattcaggg aactggcagt tgaacaaaac
     1681 gccagtccga agactggcgc agtgataaaa cattctgcat catgccacat tctgctcctg
     1741 aagatgacgc agacgatggt cccagtttcc caggtaatgc ccgggagtca gcagtacgcg
     1801 gcgtcgctca ttacagagcg agttaccaat caccacttct ccggccacac cacacaacga
     1861 cagttgtata tacgccatgc cggctgccag cgggtcaata tccgttgccg acacccacat
     1921 cctgtggtat gccggatacc ccgaacggtt caggatgtca gccattgcca gaatcatact
     1981 gccggcacca caggcaggtt cactgagcgt gataaagggt ttgttctcaa acaacgcctt
     2041 cacatctccc atctgcatct gtgccatcat gctggcaata ctccagggcg tgaaaaactg
     2101 actccggtat ttatccccca gttccagttg catgaacaca ctccccagaa aatcacagaa
     2161 tccctgctcc agtccctcaa tgacatgagc aagcaaatgc gcaatgtgga taacatcctg
     2221 cttttcataa ccactgacga ttcgcagata cttctgttcc cgggcctcac tgaactgaag
     2281 ccggttttcc agggcaatca cactacagct gacaaagtct tcaaacacct tatgacgttt
     2341 gtgataacag gctgtccggt taaacaacga aatgaactcc ttctgatggt cagtcatggt
     2401 ggccatgttt tctccgtaaa aacagggaac acatggcccg gacaggggaa caggtgttcc
     2461 ccttcgggtt gaaatgttga taaaacgggc atcacggaca tgatacccgg aggttaatta
     2521 ctgggctgag accagaggaa ccggtaaagg tgcggctttc gtctgttctt cag
//
LOCUS       DAZGQL010000239         2489 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.241, whole genome shotgun sequence.
ACCESSION   DAZGQL010000239 DAZGQL010000000
VERSION     DAZGQL010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.241"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(139..900)
                     /locus_tag="ACHKIR_005172"
     CDS             complement(139..900)
                     /locus_tag="ACHKIR_005172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HFO9992758.1"
                     /translation="MKKIIAVSALAVVGMFSGLALADRGKTGFYMTGKAGASVVTQTD
                     QRFRQDFGDDVYKYKGGDKNDTVFGAGLAVGYDFYQHYNVPVRTEVEFYGRGAADSRY
                     TLDTWYSPMVGGGREDTQNRLSVNTLMVNTYYDFRNSSAFTSSAFTPWVSVGLGYARI
                     HHKATYTDTSWNKSGEVSNFSALHYPGYDNNFAWSFGAGVRYDVTPDIALDLSYRYLD
                     AGDASVSYKDEWGDKYKSEVDVKSHDIMLGVTYNF"
     gene            1690..>2489
                     /gene="subA"
                     /locus_tag="ACHKIR_005173"
     CDS             1690..>2489
                     /gene="subA"
                     /locus_tag="ACHKIR_005173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="subtilase AB5 cytotoxin subunit A"
                     /protein_id="HFO9992759.1"
                     /translation="MLKNLRLHILFLLFISVSVRAEKPWYFDAIGLTETTMSLTDKNT
                     PVVVSVVDSGVAFVGGLSDSEFAKFSFTQDGSPFPVKEPEALYIHGTAMASLIASRHE
                     VYGVYPHALISSRRVIPDGVQDSWIRATESIMSNVFLAPGEEKIINISGGQKGISSAS
                     VWSELLSRMGRNNERLIVAAVGNDGADIRKMSAQQRIWPAAYHPVSSVNKKQDPVIRV
                     AALAQYRKGETPVLHGGGVTGSRFGNGWVDIAAPGQNITFLRPDGKTG"
BASE COUNT          714 a          565 c          559 g          651 t
ORIGIN      
        1 atgaagagaa taaaataata agaaaaacag aaatatcctg taaagcagat aacagacaac
       61 cctgcagtat aattacggca gggagtttcc gtttaattac agaagacagg taattattat
      121 ctttcaggag tagtttcgtc agaagttata agtcacacca agcatgatgt catgactttt
      181 aacatcaact tctgatttat atttatcgcc ccactcatcc ttgtaactca cactggcatc
      241 acctgcatca agatagcgat agctgaggtc cagagcgata tccggggtta cgtcatagcg
      301 gacacctgcc ccaaaactcc atgcaaaatt gttatcatat cccgggtaat gcaacgcaga
      361 aaaattgctc acctcaccag atttattcca ggaggtatca gtatatgttg ccttatgatg
      421 tatccgtgca taacccagtc caacagacac ccatggagtg aatgcactgc tggtgaatgc
      481 actgctgttt ctgaagtcat aatacgtatt caccatcaga gtattcacac tgagcctgtt
      541 ctgtgtgtct tcccgaccac caccgaccat cggagagtac catgtatcca gtgtataacg
      601 ggagtccgca gctccgcggc catagaattc cacttccgtg cgcacaggaa cattgtaatg
      661 ctgataaaaa tcatagccca ccgcaaggcc ggcaccgaat accgtatcgt tcttatcgcc
      721 gcccttatac ttataaacat cgtccccaaa gtcctgacga aagcgctggt cagtctgagt
      781 cacaaccgaa gcaccagctt taccggtcat ataaaagcct gttttgcctc tatcagccag
      841 ggccaggcct gaaaacatgc ccaccacagc aagcgctgaa accgcaataa tctttttcat
      901 ttcagttatc tccgctatca ctttccccga acgataaacg tggttgctta ttctaaaccc
      961 atgcaaaaga cagggtaaaa agagaaaaat cgatcgaatc gatcgacaaa atcgatcatg
     1021 catgatgaag atttcttatc gaatacataa aaatagtgat ggcaatcgtg gcaggagctt
     1081 acgtcaggcc ctgaagtact gacacaggca ttgatggcag gtgatataag gagtgctcca
     1141 gggaatgact cccgactgac ccaggcactg tcccgttgtc aacccgtatg gttactgctc
     1201 tgactcattt cctgtcgtca gacgttcaac tctggaccac tggttatgac gacaggtgtc
     1261 gctcatttgt cagcactata acggtcaaag atcgtggcat tgacacattc tccagaggta
     1321 aatttctcaa gaggtaacaa ctctgccagg aggatatctg cagagagtta cattcgtttg
     1381 ggttcatatc tgtgccgcgc gttttccttc catcagtgaa caaacattca gtcccatgac
     1441 gaccgggcaa atagcgtgtt cacagatatt gctgtccagg tacgaagtac cataatggat
     1501 attggtaagt tattgggtga cccccctcca tgaaaaaacc aaatataaaa atgaatgttt
     1561 cgggagagac ttcactcgct ttattgatag tgattgttta tcttttactg aagacaggat
     1621 atttcagatg tgatatccct gtaacatatt gaccagcaat actttttatt tgtaccactg
     1681 gaggatattg tgctgaaaaa tttacggctg catattctgt tcctgctttt tatctcagtg
     1741 tcagtcaggg cggaaaaacc atggtatttt gatgcaattg gtctgacaga aacaaccatg
     1801 tcgctcacag acaaaaatac cccggttgtc gtatctgttg ttgattccgg agtggcattt
     1861 gtaggaggcc tcagcgacag cgaatttgcc aaattcagtt ttactcagga tggttcgcca
     1921 ttcccggtaa aagagcctga agctttatat attcatggta ctgccatggc ttcactcatt
     1981 gcctcacgtc atgaggttta tggtgtttat cctcatgcac tgatatccag cagaagggtt
     2041 attcctgacg gtgtacagga ctcatggatt agagcaactg aaagcattat gtcaaatgtt
     2101 tttcttgcac caggagaaga gaaaatcatt aatatatcgg gaggccagaa ggggatatct
     2161 tccgcatcgg tctggtccga actgctttcc cgtatgggcc ggaataatga gcgattaatt
     2221 gttgcggcag tgggtaatga tggcgcagat atacgaaaaa tgagtgctca gcagagaata
     2281 tggcctgcgg cttaccatcc ggtcagcagt gtgaataaaa agcaggatcc tgtgataaga
     2341 gtggcagctc tggcacagta ccggaaagga gaaacacctg tattgcatgg tggaggggtc
     2401 accggaagtc ggttcggaaa cggctgggtt gatattgccg caccgggaca gaatattaca
     2461 ttcctcagac cagatggaaa aacggggat
//
LOCUS       DAZGQL010000240         2471 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.242, whole genome shotgun sequence.
ACCESSION   DAZGQL010000240 DAZGQL010000000
VERSION     DAZGQL010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2471
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.242"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..352
                     /locus_tag="ACHKIR_005174"
     CDS             <1..352
                     /locus_tag="ACHKIR_005174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFO9992760.1"
                     /translation="SLTLKQATAYSDISTRRALSEANRYTDNKFRNLSNKIERAEKRL
                     NAGIAGVTAISSIPYVAENSFSYGIGVGNYQNANAVAAGIQYKTSRNTNIRLNISWDS
                     SHNTAIGAGVAGGW"
     gene            complement(1278..1520)
                     /locus_tag="ACHKIR_005175"
     CDS             complement(1278..1520)
                     /locus_tag="ACHKIR_005175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992761.1"
                     /translation="MMMARDITAYHLNSVHISARRFPGIFQWATGMQTKNLPLTNSLL
                     QNDYAESIFVFNGERDVSEMPLPEEMLLTGNAESER"
     gene            1760..2008
                     /locus_tag="ACHKIR_005176"
     CDS             1760..2008
                     /locus_tag="ACHKIR_005176"
                     /inference="COORDINATES: protein motif:HMM:NF017472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992762.1"
                     /translation="MRKISVSYICKSLFLYCSASLPVLSANGIPDKMIVELGDNSAAK
                     ANFAVAVGQYSQANAEKSISLGASARAIENNSTALGAK"
     gene            2097..>2471
                     /locus_tag="ACHKIR_005177"
     CDS             2097..>2471
                     /locus_tag="ACHKIR_005177"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO9992763.1"
                     /translation="MQKNVTPGRRKGCPNYPPEFKQQLVAASCEPGISISKLALENGI
                     NANLLFKWRQQWREGKLLLPSSESPQLLPVTLDAAAEQPESLAEAPETLSISCEVTFR
                     HGTLRFNGNVSEKLLTLLIQELK"
BASE COUNT          751 a          580 c          505 g          635 t
ORIGIN      
        1 ttcactgaca ctgaaacagg caacagccta cagcgatatc agtacccgcc gggcactgtc
       61 agaggccaat cgttataccg acaacaaatt cagaaacctg agcaataaaa ttgaacgggc
      121 agaaaaaaga cttaatgcag gcatcgccgg agtgacagca atatcctcca tcccctatgt
      181 ggctgaaaat tctttttcat acgggatcgg cgtgggtaac tatcagaacg ctaatgctgt
      241 ggccgcaggc attcagtata aaacatcacg taataccaat atacgcctca acatctcctg
      301 ggactcatct cataacaccg cgattggtgc aggggttgcc ggcggctggt aaataatctt
      361 gcgtacatac ggcattatgg tattccgtaa tcacattaat tcagtacgca acacagcccc
      421 atccggaaca gaccactgga ctgagcggga taatcaagga aaaagggcgc gcagtgtggt
      481 cagccttcac aacaatccgg cggcagattt cctgccgcca tgttgcccgt aaacatttaa
      541 gttcgcaggc taacattatt gccacaagcc ccgtatttcc ctggtgaatc ctcaaagcga
      601 gtccctcacc atccagacaa aacacagtat cgtcgattaa taatcactaa aagcgttcgt
      661 cgtagcatca taccccctga atgaacacat ccctgtttgc aaggattctg ttgttctgat
      721 atcgacgagt agctgcaact ctgtatccgc acttattgca atcagtgcag tctggccacg
      781 atgcgattcg ctgacctgag ccttattctc caggcggact ttatgcaatg ctcccctgaa
      841 atcttccatt attttacatg tacaggcatc atattaaata tggaaataat aacgaacgga
      901 aaagggacac aatgaatatc aaagcaccag tataaaccag gatgttttga gcaatttctt
      961 actctcctga atacacactg taaatcatta gaaactgcac tagccatgct aagtacactc
     1021 tgctacgaac tgatgaggaa gtgcggtatc aattacctgg acagcctgat caacggagag
     1081 gtatatctat tatctatgta attacagggg gactgatatc agcgaaaaag caggtcagca
     1141 tattcactgc ccctggtctg aactgaaaca acgtcagcca gaaataatcc tataatgcct
     1201 catataagtg gataactttt catccgtcgt tgacatcata cacataaaac attgataaca
     1261 gtcatgaata caccgaatta tctttctgac tcagcgtttc cagtcagtag catttcttcc
     1321 ggtaaaggca tttccgaaac gtcacgttcg ccattaaata caaaaatact ttctgcgtaa
     1381 tcattctgta acaaagaatt agttaacggc agattttttg tttgcatacc ggtggcccac
     1441 tgaaaaatcc cggggaaacg acgggcactg atgtgtacag aattgaggtg gtacgcagta
     1501 atatctcttg ccatcatcat aagatggcac atcatatccc gaacgcaaac ctggcacatg
     1561 atgaacagac ggaaggctca gcaggacttc tggataatta tacacgatag ccagttactg
     1621 ctgacctgga tttattatca taccacatta tttatttcaa aaaataaatt ttgaattact
     1681 tgtttttact tgtatataaa tatccaacga tggttctgtt tgtattatgt atactatata
     1741 aaagagatgg tgaattcaaa tgcgtaaaat cagtgtcagt tatatatgca aatcattatt
     1801 tctctactgc tcagcttctt tacctgtctt atctgcaaat ggtattcctg ataaaatgat
     1861 cgtggaactt ggtgataaca gtgcggcaaa agctaatttc gctgttgctg ttggtcaata
     1921 ttcacaggca aatgcagaaa aaagtatttc tttaggtgca tcagcaagag ctattgaaaa
     1981 taactctacc gcgttaggtg caaagtaagc gtacagcctg aaccgtctgg tcagaatctg
     2041 acgaattaga caaagtggtg tccaccaaat aagtagtggg aaccaaagta tcagatatgc
     2101 agaaaaatgt gactcccggc aggcgaaaag gctgccctaa ttatcctccc gaatttaaac
     2161 agcagctcgt tgctgcctcc tgtgaacccg ggatatccat ctcaaaactt gctcttgaaa
     2221 atggcattaa cgccaatctg ttgttcaaat ggcgacaaca atggcgcgag ggaaaactgc
     2281 tattaccttc ttcagagagc ccccagctac ttcctgtgac tctcgatgcc gctgccgaac
     2341 agccagaatc gctcgcagag gccccggaaa ccctcagtat cagctgtgag gtaacgttcc
     2401 ggcacgggac gctccgcttc aatggcaatg tcagcgaaaa gctcctgact ctgctgatac
     2461 aggaactgaa g
//
LOCUS       DAZGQL010000241         2444 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.243, whole genome shotgun sequence.
ACCESSION   DAZGQL010000241 DAZGQL010000000
VERSION     DAZGQL010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2444
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.243"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..224)
                     /locus_tag="ACHKIR_005178"
     CDS             complement(<1..224)
                     /locus_tag="ACHKIR_005178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992764.1"
                     /translation="MKDEYISAYRRRITVAALRRMKRKTGSNLLTVKRHDGELTTIEI
                     TEQFISQLLLRFEGVTRGEFGRKDGETAIRT"
     gene            complement(399..599)
                     /locus_tag="ACHKIR_005179"
     CDS             complement(399..599)
                     /locus_tag="ACHKIR_005179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001539127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992765.1"
                     /translation="MKTPLIMLEEVAAEIKENTSMLEFIFKNSGDNGETDDFLLCMIR
                     SMNKTCEKAYEYVDALRTNKGN"
     gene            complement(596..787)
                     /locus_tag="ACHKIR_005180"
     CDS             complement(596..787)
                     /locus_tag="ACHKIR_005180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992766.1"
                     /translation="MKMFKGLTNEPETAFHHIAVLLEAGLIISASGDEECDELSDDIF
                     LLAQQYARSACDAFKEQRT"
     gene            complement(789..1166)
                     /locus_tag="ACHKIR_005181"
     CDS             complement(789..1166)
                     /locus_tag="ACHKIR_005181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000107960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO9992767.1"
                     /translation="MSTSQLMLIEQVESLLHESLESVSERNRHVLLEVGIEKIQQLKH
                     ILLPPRNKKDCRDGIRTQRESCGLSTAELATLLGVDEETIIQWENGKAEPLASQVIPL
                     ANILDCDPMWLLTSFHSDTTKES"
     gene            complement(1252..1911)
                     /locus_tag="ACHKIR_005182"
     CDS             complement(1252..1911)
                     /locus_tag="ACHKIR_005182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000349381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFO9992768.1"
                     /translation="MDHDSFGGRLLMRRVQLGMSQDDLAKLTGVSRVTISKIELGDSQ
                     DTRSSNLFRIADALKCSPKWLLDGSAPSPTDSIQNVRNPKPVSPNRYRYPKLTWVSAG
                     PWNCEDYIAYPEEWIGSDIYAGDHGFWLEVKGDSMTSQNGEFSIFEGMNILVNPDKEV
                     FSGKYVVARLKNSGEATLKQYIEDAGQCFLKPLNPRYPLIPINGDCEIIGVVVEMRMQ
                     I"
     gene            2251..>2444
                     /locus_tag="ACHKIR_005183"
     CDS             2251..>2444
                     /locus_tag="ACHKIR_005183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001539121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992769.1"
                     /translation="MSPDYVQMEMPARYSQADSEWIKRQLQSLPSSLRQKVALKYAEV
                     YEVTFDSEPVSFRKENRARH"
BASE COUNT          628 a          533 c          526 g          757 t
ORIGIN      
        1 gtgcgtattg ctgtttcacc atctttccgc ccaaactcac cacgggtaac gccttcaaat
       61 cgtaatagca actggcttat gaactgttct gttatttcaa tagttgttaa ctctccatca
      121 tgacgcttta cagtgaggag attacttcct gtttttcgtt tcatccgccg cagtgctgca
      181 accgttatgc ggcgacgata tgcagaaatg tattcatctt tcatttgtta tttcccgtat
      241 gcctttttga gaaacaatac tgcaactgac cagtatccgg catcagccat taataaagcg
      301 gttttatagg catgtttgtc tttcatgtgt catccccatt ttaaaatagt gagaactccc
      361 gaccgcagac cgtcatgttt ttatgtatga ttgtctgttt aatttccttt attcgttctc
      421 agagcatcaa catactcgta agccttttca caggttttat tcatggaacg aatcatacaa
      481 agtaagaaat catctgtctc tccgttatca ccagagtttt taaatataaa ctcaagcatt
      541 gacgtgtttt cttttatttc tgctgccact tcctcaagca taatcagggg agttttcatg
      601 ttctttgttc cttaaatgca tcgcatgcgc ttctggcgta ttgttgtgcc agtaaaaata
      661 tgtcatccga aagttcatca cattcttcat caccggaagc cgaaatgatt aaccccgctt
      721 caagcagtac tgcaatgtga tgaaaagctg tttccggttc gttggtgagg cctttgaaca
      781 ttttcatctt acgattcctt tgtcgtgtca gaatggaaac ttgtaagtaa ccacatcgga
      841 tcgcagtcaa gtatatttgc cagaggaatc acctggcttg ccagaggttc agccttgccg
      901 ttctcccact ggatgatggt ttcttcatca actccaagca gcgtggcgag ttcagcagtg
      961 gatagaccgc agctttcgcg ttgggtgcgg atgccatcac ggcaatcttt tttgtttctc
     1021 ggaggtaaca gaatatgttt tagttgttgg attttttcga ttccaacctc aaggagtacg
     1081 tgacggtttc tttccgatac agactccaga ctttcatgaa gcagcgattc tacttgttcg
     1141 ataagcatca attgtgaagt agacataaaa aaacctcgta accaatattt cgaaatctag
     1201 gttacgagtg agtaatgtag gtgtcaagag caattaatgt tttttgttga attaaatttg
     1261 catacgcatc tcaacgacaa caccaataat ttcacaatcc ccattgatgg ggatcagtgg
     1321 atagcgtggg tttagtggct ttaggaaaca ttgcccagcg tcttctatgt actgtttaag
     1381 ggttgcctct ccggagtttt tgagcctggc gacgacatat tttcctgaaa aaacttcttt
     1441 gtcagggtta acaagaatgt tcatgccttc aaaaatagag aactctccat tctgtgatgt
     1501 cattgagtcc cctttcactt ccagccagaa gccgtgatcg cccgcatata tatcgctacc
     1561 aatccattct tctggataag ctatgtaatc ttcgcagttc caggggccag cactcaccca
     1621 tgttagtttc ggatatctat aacggtttgg agaaactggc ttaggatttc ggacgttttg
     1681 aatgctatct gtaggtgatg gggcgctgcc gtcaagaagc catttaggag agcatttcaa
     1741 tgcatccgcg attctaaata ggtttgaaga tcttgtgtct tgtgagtctc ctaactctat
     1801 tttgctgatt gttacacgcg agacaccggt caattttgcc aggtcatctt gtgacattcc
     1861 tagctgtact ctgcgcatta acagtctgcc accaaagcta tcgtgatcca tcttctcccc
     1921 cttcttgtcg aaataatcgt ttcgaaacaa atatagcaaa aaatcttgaa atctaggttt
     1981 cggaaaatta agattaatcg aaacaaacgt gtcgaggtgg atatggattt gtatgaaatt
     2041 ctaaaaaaca tgtttggatc gaatgttgaa ataggtcgtt attttccccg aaggggaagg
     2101 gctagaaccg gacaggctgt aggtaagtgg aaaacccgag gtgtgccgga agacgtggcg
     2161 atcctttgtc atctggatcc caaaattcct tatcagcatc cgtcgctcat gaatgccagt
     2221 catgaatcgt gatgttctgc cggaggctgt atgtcccctg attacgttca gatggagatg
     2281 cctgccaggt acagccaggc agattcagag tggattaagc ggcagttaca gagcctgcct
     2341 tcctcactga gacagaaggt cgccctgaaa tacgcggagg tttacgaagt tacttttgat
     2401 tccgagcctg tttcattccg taaggagaac agagcgaggc atga
//
LOCUS       DAZGQL010000242         2433 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.244, whole genome shotgun sequence.
ACCESSION   DAZGQL010000242 DAZGQL010000000
VERSION     DAZGQL010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2433
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.244"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(231..440)
                     /gene="fumD"
                     /locus_tag="ACHKIR_005184"
     CDS             complement(231..440)
                     /gene="fumD"
                     /locus_tag="ACHKIR_005184"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HFO9992770.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
     gene            795..989
                     /locus_tag="ACHKIR_005185"
     CDS             795..989
                     /locus_tag="ACHKIR_005185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992771.1"
                     /translation="MDCRTQRLKNRHSRWAETQAHIPLHAFSMSPILRARHHHFRNTG
                     FAFRQCAQKASFSHPSQLKD"
     gene            997..2409
                     /gene="pykF"
                     /locus_tag="ACHKIR_005186"
     CDS             997..2409
                     /gene="pykF"
                     /locus_tag="ACHKIR_005186"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HFO9992772.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
BASE COUNT          690 a          584 c          519 g          640 t
ORIGIN      
        1 aaagcaagaa attataagtg aactgatatt tattatcatt tgaaataaat ttaacttaac
       61 cgacaattat ttgtgaagtt catcgcagaa aataaaaata actttatcgc tataaattaa
      121 aatacacacc gaaattcgta taatttcagc agttgttatt tagaatgatt aactggaccg
      181 caactgaagc aagaatatca ggatgaagct taccgcctca tcctgaatgg tcattttacc
      241 agtgcgttaa tgacggtttc catcgcctgc ttttccagct cactgcgctg aatgcgttta
      301 ttggctaatg ctgtacgtaa tacgcctgcg ataacaatat gctttggttc ctgccccaga
      361 tcgcgcattt ccagcacaac tttaccgaca actctgcaca tttctcggta cagttcatcc
      421 tcttttgtcc gatttcccat tctttatacc tatttatcat gccaactatc agcatatatc
      481 aatctaacca attaaacaaa aatcaaacaa aatcagacaa ataacgcgat aaattatttt
      541 aactgtagca attgagcgat gatgtattta tacaccggat gaactttcac ttatcttcac
      601 agtgacaacc ccggcaccag acgttgcgca aacagcgaag tttttgcgta accttttccc
      661 tggaacgtta aatctttgat aacaatttat tgtctaacaa gttgtatatt ttttgaaacg
      721 ctgtttttgt tttccttttg gattaatttc agcgtataat gcgcgccaat tgactcttga
      781 atggtttcag cactttggac tgtagaactc aacgactcaa aaacaggcac tcacgttggg
      841 ctgagacaca agcacacatt cctctgcacg ctttttcgat gtcacctatc cttagagcga
      901 ggcaccacca ctttcgtaat accggattcg ctttccggca gtgcgcccag aaagcaagtt
      961 tctcccatcc ttctcaactt aaagactaag actgtcatga aaaagaccaa aattgtttgc
     1021 accatcggac cgaaaaccga atctgaagag atgttagcta aaatgctgga cgctggcatg
     1081 aacgttatgc gtctgaactt ctctcatggt gactatgcag aacacggtca gcgcattcag
     1141 aatctgcgca acgtgatgag caaaactggt aaaaccgccg ctatcctgct tgataccaaa
     1201 ggtccggaaa tccgcaccat gaaactggaa ggcggtaacg acgtttctct gaaagcgggt
     1261 cagaccttta ctttcaccac tgataaatct gttatcggca acagcgaaat ggttgcggta
     1321 acgtatgaag gtttcactac tgacctgtct gttggcaaca ccgtactggt tgacgatggt
     1381 ctcatcggta tggaagttac cgccattgaa ggtaacaaag ttatctgtaa agtgctgaac
     1441 aacggcgacc tgggtgaaaa caaaggtgtg aacctgcctg gcgtttccat tgccctgcca
     1501 gcactggctg aaaaagacaa acaggacctg atctttggtt gcgaacaagg cgtagacttt
     1561 gttgctgctt cctttattcg taagcgttct gacgttatcg aaatccgtga gcacctgaaa
     1621 gcgcacggcg gcgaaaacat ccacatcatc tccaaaatcg aaaaccagga aggcctcaac
     1681 aacttcgacg aaatcctcga agcctctgac ggcatcatgg ttgcgcgtgg cgacctgggt
     1741 gtagaaatcc cggtagaaga agttatcttc gcccagaaga tgatgatcga aaaatgtatc
     1801 cgtgcacgta aagtcgttat cactgcgacc cagatgctgg attccatgat taaaaaccca
     1861 cgcccgactc gcgcagaagc cggtgacgtt gcaaacgcca tcctcgacgg tactgacgca
     1921 gtgatgctgt ctggtgaatc cgcaaaaggt aaatacccgc tggaagcggt ttctatcatg
     1981 gcgaccatct gcgaacgtac cgaccgcgtg atgaacagcc gtctcgagtt caacaatgac
     2041 aaccgtaaac tgcgcattac cgaagcggta tgccgtggtg ccgttgaaac tgctgaaaaa
     2101 ctggatgccc cgctgatcgt ggttgctacc cagggcggta aatctgctcg cgcagtacgt
     2161 aaatacttcc cggatgccac catcctggca ctgaccacca acgaaaaaac ggctcatcag
     2221 ttggtactga gcaaaggcgt tgtgccgcag ctggttaaag agatcacttc tactgatgat
     2281 ttctaccgtc tgggtaaaga actggctctg cagagcggtc tggcacacaa aggtgacgtc
     2341 gtagttatgg tttctggtgc actggtaccg agcggcacta ctaacaccgc atctgttcac
     2401 gtcctgtaat attgcttttg tgaattaatt tgt
//
LOCUS       DAZGQL010000243         2418 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.245, whole genome shotgun sequence.
ACCESSION   DAZGQL010000243 DAZGQL010000000
VERSION     DAZGQL010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.245"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..1076
                     /locus_tag="ACHKIR_005187"
     CDS             <1..1076
                     /locus_tag="ACHKIR_005187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFO9992773.1"
                     /translation="SPSIRPSLYAYGANATTLKPFSVALGAGSVADRGAGIEAYMPET
                     KRWNPGDVVKSRMSDKQLNRLMEIDKLISAYNSQETLSAEETKQLEEVKKERADMFAT
                     YIATHDAVSVGVGATTTGRHAVTRQITSVAAGSVDTDAVNVAQLRDLQTVTEKADAAL
                     DKRITNNTSAITKNLQVVNSNKEEISNINKTTKILDERVTANTQTAEEHKLLISENSK
                     QISTNTLGIRENTQRVQDEGQARIRGDRETLRAATGYTDMRVNGLERNTNKRFAQLKE
                     DIDKNRKRADAGIAGAMAMTGIPSVPGKKVSFGMAMAGYRSEGAVAAGFHFNTSENSA
                     VKVNAAWDTENGAGVSAGMALGW"
     gene            complement(1506..2081)
                     /gene="dolP"
                     /locus_tag="ACHKIR_005188"
     CDS             complement(1506..2081)
                     /gene="dolP"
                     /locus_tag="ACHKIR_005188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001238641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HFO9992774.1"
                     /translation="MRLLYSAVVLISALLLQGCVAATVVGTAAVGTKAATDPRSVGTQ
                     VDDEVLELRVSSALSKDEQIRKEARISEIAYQGRVLLIGQSPDRELSARARQIALGVD
                     GVQEVYNEIRQGTPVSPVIMAGDAWLTTKIRSQLLTSARVKSSSVKVVTENSEVFLLG
                     LVTEQEGRAAADIASRVSGVKRVTTAFSCIR"
BASE COUNT          695 a          567 c          592 g          564 t
ORIGIN      
        1 catccccgtc aatacggccc tcgctgtacg cttacggtgc aaacgcaaca acattgaaac
       61 cattctctgt cgctctgggc gcaggaagtg ttgctgatcg tggcgctggt attgaagcat
      121 atatgccaga gacaaagcgc tggaatccag gggatgtagt aaaatccagg atgtctgaca
      181 agcagcttaa caggcttatg gaaatcgata agctaatatc cgcttataat tcacaagaga
      241 cgctgtctgc tgaagagact aaacaactgg aggaagtgaa aaaagagcgg gctgatatgt
      301 ttgcgacata tatagcaacg cacgatgctg tatctgtagg tgttggtgca acgacaacag
      361 gacgacatgc tgttacaaga cagataacta gcgttgcagc aggttctgtc gatacggatg
      421 ctgttaacgt tgcacagctc agggatttgc agactgtgac agaaaaggca gatgcagcgc
      481 ttgataaacg cataacaaac aacaccagtg cgataactaa aaatctgcag gtggttaaca
      541 gtaataaaga ggaaatcagt aacattaata aaacgacaaa aattctggat gaaagagtga
      601 cagccaatac acagacagca gaagaacata agctgcttat ttcggagaac agcaaacaaa
      661 tcagtaccaa cactcttggt atcagagaga atacacaacg ggtccaggat gaaggacagg
      721 ctcgaatccg gggagacagg gaaacactgc gtgcagcgac cggttatacc gacatgcggg
      781 taaacgggct ggagcgtaac acgaataaac gttttgcaca gcttaaagag gatatagaca
      841 aaaacagaaa acgtgcagat gcaggtattg caggtgcgat ggcaatgacg gggataccgt
      901 ccgtccccgg gaaaaaggtc tcatttggta tggccatggc cggataccgg agtgaaggtg
      961 ctgttgctgc cggcttccat tttaatacaa gtgaaaacag tgctgttaaa gtgaatgcag
     1021 cctgggacac tgaaaatgga gctggcgttt cagccgggat ggctctggga tggtaatgct
     1081 tccgggccag aagccgttcg cagatattgt tatccagggg acactgcacc accattcagg
     1141 aagcgattca gtttatcgca tcggctcagg atgtaattaa tacggacaat ctggctcctg
     1201 caccggagcc agacgtacac gtgtcaacgc cacgatcttt gacctgtatg gataaaagct
     1261 cagattattt accagcagcg acatccggag agcaccggga ttttatctgt gagtgaacat
     1321 acccgggcaa aaaaatcccc cgaattcagg ggggtgtcaa agaggaatta tgtatattgg
     1381 ttattataat gagcacgtct ccggtattcc gcgacgaatg ttcacgtggt ggcatgaacg
     1441 gcccggaaac gtgctcttct gatttttaac gtaaatttgc actaaaggca actcagatac
     1501 ggccttcacc gtatacagga gaatgccgtc gtgacccgct tcacgccact caccctactg
     1561 gcaatatctg ctgccgctcg cccttcctgc tctgtaacca ggcccagcaa aaagacttcg
     1621 ctgttttctg tcaccacctt gacactggat gatttgacac gggcacttgt gagtaactga
     1681 gaacgaattt ttgtcgtgag ccaggcatca cctgccatta tgaccggact gacgggagtc
     1741 ccctgccgga tttcattata gacctcctga acaccatcga caccgagtgc tatctgacgg
     1801 gcacgggcag ataattcacg gtccggagac tgaccgatca gcagcacgcg tccctgatat
     1861 gcaatctcac tgatgcgtgc ttcttttctg atttgttcat cttttgaaag tgcactgctg
     1921 accctcagct ccagcacttc atcatccacc tgcgttccga cactgcgtgg gtcggttgct
     1981 gcctttgtcc cgacagcggc tgtacccacc acagttgcag caacgcatcc ctgtaacaat
     2041 aaggctgaaa tcaggacaac agcagaatat aataaacgca tatctccccc ggaatttatc
     2101 gtacaaaaag tttaccttca gtgaaacaaa acaagctacg gactgttatt ctcttccggc
     2161 acagccggaa acagcggata cgttacgtga catatctgta tctgccagac ggaaatgttt
     2221 atgacggata tggatgtact gaaatttaat attgttcatg tttttgcacg aaaacgttga
     2281 tagcgtagta aagattctgt tttttaacta cctggtttgc tgagcaggat ctccttttcg
     2341 ttatacacag gctaaaaggc accgtggccc cccggtgcct ttttcataaa acagagaaaa
     2401 cacggctttg aatatacc
//
LOCUS       DAZGQL010000244         2417 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.246, whole genome shotgun sequence.
ACCESSION   DAZGQL010000244 DAZGQL010000000
VERSION     DAZGQL010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2417
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.246"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..490
                     /locus_tag="ACHKIR_005189"
     CDS             <1..490
                     /locus_tag="ACHKIR_005189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992775.1"
                     /translation="ICKKMVPFYSDDNCLLCNKKNEELSPTRDEINKIREFDVTISQD
                     NLNSVTQRLLPTPLAAVRARRVAFENPYRNKVLSESLPAGNLLYRSALAFIQALIALV
                     PSKKILDLDKITDALRRRYVHLEQLEYAELRNALACALSTCYNRKYLIKVAKGKYLPV
                     DD"
     gene            572..919
                     /locus_tag="ACHKIR_005190"
     CDS             572..919
                     /locus_tag="ACHKIR_005190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO9992776.1"
                     /translation="MPGQPIPSELKEKGLIWLLPPYCWSHRQIARKLKISASVVSKWR
                     YELVEQGLLPENEKITSVDVEDWTPERRFSVVLETATLSEMELADYYRRMGLFVEQVK
                     EWRAISIQAHEKN"
     gene            1092..1256
                     /locus_tag="ACHKIR_005191"
     CDS             1092..1256
                     /locus_tag="ACHKIR_005191"
                     /inference="COORDINATES: protein motif:HMM:NF024776.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO9992777.1"
                     /translation="MERRKIIALVREAVSNGARKSQACRCIGISIRTLQRWTDGNVIT
                     TDVSVNGAPY"
     gene            complement(1260..1727)
                     /locus_tag="ACHKIR_005192"
                     /pseudo
     CDS             complement(1260..1727)
                     /locus_tag="ACHKIR_005192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1791..2075
                     /locus_tag="ACHKIR_005193"
     CDS             1791..2075
                     /locus_tag="ACHKIR_005193"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO9992778.1"
                     /translation="MEQKTLSAEPRRSFSNNFKLQMVKLALQPRVSVARIARKHDIND
                     NLLFKWLRLWLIKGRISRRLPVRNSSGIGVELLPVEMTLDERPYPAFYAR"
     gene            complement(2292..>2417)
                     /locus_tag="ACHKIR_005194"
     CDS             complement(2292..>2417)
                     /locus_tag="ACHKIR_005194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011815933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFO9992779.1"
                     /translation="LLITFLPVFLLPVLGNIGFANTTFSDADFGALGILLGIIVR"
BASE COUNT          714 a          530 c          551 g          622 t
ORIGIN      
        1 catatgtaaa aagatggtac ctttttattc tgatgataat tgtttgcttt gtaataagaa
       61 gaatgaagag ttgtcaccaa ccagagatga aattaataaa attcgcgagt ttgatgtaac
      121 aatatcccag gacaatctta attcagttac tcaacgattg ttacccacac cattagctgc
      181 ggtaagagcc cgaagagttg catttgaaaa tccatataga aataaagtac taagtgaatc
      241 attacctgct ggaaaccttc tttacagaag tgcattagct tttatacagg ccttgattgc
      301 attagtccca tcaaagaaaa tactggactt ggataagata acagatgctc tgcgtaggcg
      361 gtatgttcat cttgagcaat tagaatatgc cgaattaaga aatgctttag cttgtgcatt
      421 gtcaacttgt tataatagaa aatatctcat taaagttgca aaaggaaaat atttacctgt
      481 tgacgactag gtgcgtatta ccgtgaagga gatcggtgag taaccccctg tgtcagcata
      541 gttgtcgcct caatttttta aggagataac aatgccagga caaccaatac caagtgagct
      601 caaagagaaa ggactgatat ggcttttacc accatactgc tggtcacatc gtcagatagc
      661 ccgtaagcta aagatcagtg cctcagttgt atcaaaatgg agatatgagt tagtggagca
      721 agggctttta cctgaaaatg aaaagattac ctccgttgat gttgaagact ggacaccgga
      781 gcgtcgattc tcagttgttc ttgagacggc tacgctgtca gaaatggagc ttgctgatta
      841 ttaccgacgc atgggattgt ttgttgaaca ggttaaagaa tggcgggcta tctcaattca
      901 ggcgcatgaa aaaaattgac ggaaaatatt cgaacagaca gggcattgcg tgacgcaaaa
      961 aataagttac gagaactgta gaaagagctt aagcgtaaag ataaagctct ggcggaagct
     1021 gcggccctgc ttatattgag ggaaaagttc aatgccctct gggacaacag cgaggaagac
     1081 tgacccctct cctggagcgg cgtaaaatta ttgcccttgt ccgcgaggcc gtctccaacg
     1141 gcgcccggaa aagtcaggct tgtaggtgta tcggtatttc aattcggaca cttcagcgct
     1201 ggactgatgg caacgttatt accacggatg taagcgtcaa cggagcaccg tattgacgct
     1261 tatttattgg tgagtactac gttccatggc aggagttcat caactcggtt ggaaggccat
     1321 tccggcagta cgctcaggat aaggcgcaga tacgcttccg gatcgatacc gttcagacgg
     1381 caggtgccga tcagcccgta caacagtgct ccacgctcgc cgccgtgatc gctgccaaag
     1441 aacataaagt ttttctttcc gagacagact gcacgaagcg ctctttccgc agcattatta
     1501 tccgcctccg ccagaccgtc atcactgtaa taacagaggg catcccactg attcagtaca
     1561 tagctgaacg cttcgcccag tctggatttt ttcgacagcg taccattctt ctccaccatc
     1621 cattcatgca gcgacgtcag taacactttg cttcgctgct gcctgacgga aagacgctct
     1681 gactccggta atccccgtat ttcgtaagcg taaaccgacc gccgtatgta gccattaagc
     1741 cagtattggt aacgtaggtg cccacctttt caaactagtg gacgcctgtt atggagcaaa
     1801 aaacattatc cgcagaaccc cgcagatcat tttcaaataa ttttaaactt caaatggtta
     1861 aactggcttt acaaccaagg gtttctgttg cccgtattgc ccggaagcat gatatcaatg
     1921 ataatcttct gttcaaatgg ctcaggctct ggctgatcaa agggcgtata tcacggcgtc
     1981 ttccggtaag gaactcttct ggcattggcg ttgaattatt acctgtggag atgacgctgg
     2041 atgagcggcc ttaccccgct ttttacgcga ggtgattatg aagatcaatg actagccgtc
     2101 gaaccgtgtg catgaactgc tgcactgaaa cttctcagct gtaaaataat ccttacgcta
     2161 cgtacttccc ggagcgctta cgattaatcg gatgcagtcc tgactggaag aaagaaaaat
     2221 gggaaaaaat aagaggaatt aatatcctcc acgttcctga aatcctggag gatattaatg
     2281 tcatcaaaat attagcgaac gataatccct aacaaaatac ccagtgcgcc aaagtcagca
     2341 tcactgaagg tggtgttagc aaaaccaatg ttacccagca caggcaataa gaatactggc
     2401 aggaaggtaa taagcaa
//
LOCUS       DAZGQL010000245         2377 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.247, whole genome shotgun sequence.
ACCESSION   DAZGQL010000245 DAZGQL010000000
VERSION     DAZGQL010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2377
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.247"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            140..1279
                     /locus_tag="ACHKIR_005195"
     CDS             140..1279
                     /locus_tag="ACHKIR_005195"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004168096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFO9992780.1"
                     /translation="MSTMKPAIFSFFSGSGFLDLGFENAGYEVVFVNEFHPPFMDAYK
                     HSRKKLGKQPPRFGYAQESIETIEHSVIKHSMKTLKGENRLVGFIGGPPCPDFSVAGK
                     NKGKDGENGKLSQSYIDLIIEMMPDFFLFENVKGLYRTLKHREFFEQIKNELASVGYI
                     LHERLINSIEYGAPQDRERIILVGFLDKSLAKNFEWSGQIYPNKEAFQFPWPTTNTYE
                     EDSSMECPQGIPEELTIEYWFEKNDVTNHPNAKHVFTARAGLARFQSVEEGDDSKKSY
                     KRLHRWRYSPTAAYGNNEVHLHPYKPRRITVAEALAIQSLPKNFELPGDMTLSNMFKT
                     VGNGVPYIAALGIAQSILQHLGYSNEDNSIESGSGDFELTQKYAV"
     gene            1215..2288
                     /locus_tag="ACHKIR_005196"
     CDS             1215..2288
                     /locus_tag="ACHKIR_005196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015953250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFO9992781.1"
                     /translation="MRITASNLVQAISSLPKNTLFNYINDRNSGKIEIVRIQHPEGPI
                     EIKRFDPKKGQTQATAKTESISTQMLWRLANALEENRPVNVERVFGASYNTRSVLESL
                     LAHTPEFYWCRPARLEIMNAQKSIKPGHKHLIFLPDMPHTNGLLIEHQTNIVVSEMSF
                     DVVHQSVDIETIKPTKGMTIEEKRRHAQIQIALVKIGYCLGLDTWVAANDRSLQYNGK
                     AIAQMDGVISSLSDEQVLQSYNDAIKDARLIDCIWFREGRMMPAVMEIEHSTGIKSGL
                     TRMKQFYDHAPRLQDIRWTIVAPDEYRAKVIEFANMPQFIELKAKFFPYSSVEELYSL
                     CDRRKPKGINDLFLDSFMEDCVI"
BASE COUNT          803 a          443 c          472 g          659 t
ORIGIN      
        1 atctaatctg aagtagtgcc aggtcttatt tgctaatata ttaaaaagta aattgataat
       61 taacaagtta tcatgtaaaa tagtaggatc gtacagcgag atatttagta aaaatttaag
      121 ctaacttgat aggtataaaa tgtccacaat gaaacccgct atattttcat tcttttcagg
      181 atcaggtttt cttgacctag gcttcgaaaa tgccgggtat gaggtggttt tcgtcaatga
      241 attccatcct cccttcatgg acgcgtataa acactcaaga aaaaaacttg gcaaacagcc
      301 gccacgcttt gggtatgccc aagaaagcat tgaaactatt gagcattcag tgattaagca
      361 tagcatgaaa acactgaaag gtgaaaaccg cctagtgggc ttcattggcg gaccaccatg
      421 ccctgacttt tctgttgctg gcaaaaacaa aggcaaagat ggtgaaaatg gcaaattatc
      481 acaaagttac attgacctga taattgaaat gatgcctgat ttcttccttt ttgaaaacgt
      541 taaaggctta taccggactc ttaaacatag agaatttttc gaacaaatta agaatgaact
      601 agcttcagta ggatatatcc tgcatgaaag attgattaat tcaattgaat acggtgcgcc
      661 tcaagatcga gaaaggatta ttttagttgg ttttcttgat aagtctttag ccaaaaactt
      721 cgaatggagt ggccaaatat accccaataa ggaagctttc cagttccctt ggccaacaac
      781 aaacacatac gaagaagact caagtatgga atgcccgcaa ggtattccag aagaacttac
      841 tatagaatac tggttcgaga aaaacgatgt taccaatcat ccaaacgcta aacatgtatt
      901 tacggcccga gccggtttag caagattcca gtcagtagaa gaaggcgacg atagtaaaaa
      961 atcatataaa aggcttcatc gctggagata ctctccaaca gcagcatatg gtaataatga
     1021 agttcacctt cacccataca aacctcgtcg tattacggtc gctgaagcat tggcaatcca
     1081 gtctttgcca aaaaattttg agctaccagg ggatatgact ttgtcgaata tgtttaaaac
     1141 agtcggaaac ggagttccct atatcgccgc gctcggtatt gcccaatcaa ttttgcaaca
     1201 tttaggatac agtaatgagg ataacagcat cgaatctggt tcaggcgatt tcgagcttac
     1261 ccaaaaatac gctgtttaac tatataaacg ataggaatag cgggaagatt gagattgtac
     1321 gtatccaaca tcccgaaggt cctatcgaaa taaagagatt tgatcctaag aaaggtcaaa
     1381 ctcaagcaac agctaaaaca gagagtattt cgactcaaat gctttggcga cttgcaaatg
     1441 cactcgaaga aaacagacct gtcaacgttg aaagggtttt cggtgccagt tacaacaccc
     1501 gctcggtatt ggagagcttg ctcgcacata ctccagagtt ctattggtgt aggccagcca
     1561 gactggaaat catgaatgca caaaagtcta taaaaccagg acataagcat ctgatttttc
     1621 tgccagatat gccacatact aatggtttgt taattgagca ccagactaat attgttgtct
     1681 ctgaaatgag cttcgatgtt gttcaccaaa gcgttgatat tgagacaatt aaaccgacta
     1741 aaggcatgac tattgaagaa aaaagacgtc atgctcagat acaaattgcc ttagtaaaaa
     1801 taggttattg tttaggttta gatacttggg tagcagccaa tgatcgtagc ttacaatata
     1861 atggtaaagc tatagcgcaa atggacggtg taataagttc attatctgat gagcaagtac
     1921 tccaatcgta taatgatgcg attaaagatg ctcgtcttat agactgcatt tggtttaggg
     1981 aaggccgcat gatgccagca gtaatggaga ttgaacacag tactggtata aaaagtggtc
     2041 tgactcgcat gaaacaattc tatgatcatg caccgagatt acaagatatc cgttggacta
     2101 tcgttgctcc tgatgaatat cgagcaaagg tgatagaatt tgcaaacatg ccccaattca
     2161 ttgaattaaa agctaaattc ttcccttact cttcagttga agaattatac tctctatgcg
     2221 accgaagaaa acctaaagga ataaatgatt tatttctaga ttcttttatg gaagattgtg
     2281 ttatatagct aagaacatca aattaacatg aacctactga tatatactat cagtaggttt
     2341 aaaatgattt caaatgtgtt ccccaatatg agctatt
//
LOCUS       DAZGQL010000246         2368 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.248, whole genome shotgun sequence.
ACCESSION   DAZGQL010000246 DAZGQL010000000
VERSION     DAZGQL010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.248"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            116..2257
                     /locus_tag="ACHKIR_005197"
     CDS             116..2257
                     /locus_tag="ACHKIR_005197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HFO9992782.1"
                     /translation="MAMFTPSFSGLKGRALFSLLFAAPMIHATDSVTTKDGETITVTA
                     DANTATEATDGYQPLSTSTATLTDMPMLDIPQVVNTVSDQVLENQNATTLDEALYNVS
                     NVVQTNTLGGTQDAFVRRGFGANRDGSIMTNGLRTVLPRSFNAATERVEVLKGPASTL
                     YGILDPGGLINVVTKRPEKTFHGSVSATSSSFGGGTGQLDITGPIEGTQLAYRLTGEV
                     QDEDYWRNFGKERSTFIAPSLTWFGDNATVTMLYSHRDYKTPFDRGTIFDLTTKQPVN
                     VDRKIRFDEPFNITDGQSDLAQLNAEYHLNSQWTARFDYSYSQDKYSDNQARVTAYDA
                     TTGTLTRRVDATQGSTQRMHATRADLQGNVDIAGFYNEILGGVSYEYYDLLRTDMIRC
                     KKAKDFNIYNPVYGNTSKCTTVSASDSDQTIKQESYSAYAQDALYLTDNWIAVAGIRY
                     QYYTQYAGKGRPFNVNTDSRDEQWTPKLGLVYKLTPSVSLFANYSQTFMPQSSIASYI
                     GDLPPESSNAYEVGAKFELFDGITADIALFDIHKRNVLYTESVGDETIAKTAGRVRSR
                     GVEVDLAGALTENINIIASYGYTDAKVLEDPDYAGKPLPNVPRHTGSLFLTYDIHNMP
                     GNNTLTFGGGGHGVSRRSATNGADYYLPGYFVADAFAAYKMKLQYPVTLQLNVKNLFD
                     KTYYTSSIATNNLGNQIGDPREVQFTVKMEF"
BASE COUNT          657 a          563 c          555 g          593 t
ORIGIN      
        1 gaatgattct caattacata tccgatggcg aaataacaaa cggatagtgc ttagtgagca
       61 ataagaaaca attttcactc gcgacaatcc gcgagttttt gggataacag actaaatggc
      121 tatgttcaca ccttcattct caggactcaa aggtcgggcg ctcttttcac tgctttttgc
      181 ggcaccgatg attcatgcaa cagactctgt aacgaccaaa gatggcgaaa caatcactgt
      241 tacagcagat gcaaataccg caactgaggc gaccgatggt tatcaacctc tgagcacttc
      301 cacggcgaca ttaaccgata tgccgatgct ggatatcccg caggtggtca atacggttag
      361 cgatcaggtt ctggaaaacc agaatgcgac aacgctggat gaggcgcttt ataacgtcag
      421 taacgtggta cagaccaata cattaggcgg gactcaggat gcctttgtac gccgtgggtt
      481 tggcgcaaac cgggatggct ccatcatgac caacggtctg cgaaccgtac ttcctcgcag
      541 tttcaacgcc gccacagaac gtgtggaagt gctaaaaggc ccggcctcca cgctgtatgg
      601 cattctcgat cctggcggac tgattaacgt cgtgaccaag cgcccggaaa aaacattcca
      661 tggttcggtt tcagccacct cctccagttt tggtggcggc actgggcaac ttgatatcac
      721 aggtcccatt gaaggcactc agctggcgta tcgccttacc ggggaagtgc aggatgaaga
      781 ttactggcga aacttcggta aagagcgcag tacatttatt gccccgtcac tcacctggtt
      841 tggtgataat gcaacagtaa ccatgctcta ttcccatcgg gactataaaa ctccgttcga
      901 tcgtggaacg attttcgacc ttacgacgaa acagcccgta aacgttgatc gaaaaatacg
      961 ttttgacgaa ccgtttaata ttacagatgg tcagtccgat ctggcgcaac tcaacgcaga
     1021 atatcatctc aatagccagt ggacagcgcg ctttgattac agctacagcc aggataaata
     1081 cagcgataat caggcgcgtg ttaccgcgta tgatgcaacg acaggaacac tgacacggcg
     1141 tgttgatgca actcagggat ctacccagcg tatgcatgct actcgtgcgg atctgcaagg
     1201 gaatgttgat attgccggat tctataatga gattctgggt ggggtgtcat atgaatatta
     1261 tgatcttctg cgtacagata tgattcgctg taaaaaagct aaagatttca atatctacaa
     1321 ccctgtttat ggtaatacca gcaaatgtac aacggtttcg gcgtcggaca gtgatcagac
     1381 gatcaaacag gagagctact cagcgtatgc acaggacgca ctctatctga ccgataactg
     1441 gattgccgtc gccgggatcc gctatcagta ttacacgcag tatgcgggta aaggccgtcc
     1501 ttttaatgtc aatactgaca gccgcgatga acaatggact cccaaactgg ggttggtcta
     1561 caaactgacg ccatcggtat ccttatttgc caattattcg caaacattta tgccgcagtc
     1621 gtcaattgcc agctacattg gcgatcttcc accggaatca tctaatgctt acgaagtcgg
     1681 ggcaaaattc gagctattcg atggtatcac cgcagatatt gcgctgtttg atatccataa
     1741 acgtaatgtg ttgtataccg aaagtgttgg tgatgaaacc attgccaaaa cggcaggccg
     1801 tgttcgttca agaggggtag aagtcgacct tgcgggagca ttaactgaaa acattaatat
     1861 cattgccagc tacggctata ccgatgctaa ggttctggaa gatcctgatt atgcaggtaa
     1921 accattgccg aatgttcctc gtcataccgg ttcgctattc ctgacctatg acattcataa
     1981 catgcctggc aataacacac tgacgtttgg cggtggtgga catggcgtaa gccgtcgttc
     2041 ggcaaccaat ggggctgact attatctgcc aggctatttt gttgccgatg ccttcgccgc
     2101 atacaaaatg aaattgcagt atccggtcac actgcaatta aacgtcaaaa acctgtttga
     2161 taaaacgtat tacacctctt ccatcgccac aaataatctg gggaatcaga ttggcgatcc
     2221 gcgtgaagtg caattcacgg tgaaaatgga attttgatca aaaaagcccg gtatgtttac
     2281 cgggctgaga ctgatgacaa actccaaatt gtctgatgcg ctacgcccat cagatctacc
     2341 atagaaattc aatatattaa attttcat
//
LOCUS       DAZGQL010000247         2278 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.249, whole genome shotgun sequence.
ACCESSION   DAZGQL010000247 DAZGQL010000000
VERSION     DAZGQL010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.249"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>2278
                     /locus_tag="ACHKIR_005198"
     rRNA            <1..>2278
                     /locus_tag="ACHKIR_005198"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          587 a          496 c          721 g          474 t
ORIGIN      
        1 aatgggtcag cgacttatat tctgtagcaa ggttaaccga ataggggagc cgaagggaaa
       61 ccgagtctta actgggcgtt aagttgcagg gtatagaccc gaaacccggt gatctagcca
      121 tgggcaggtt gaaggttggg taacactaac tggaggaccg aaccgactaa tgttgaaaaa
      181 ttagcggatg acttgtggct gggggtgaaa ggccaatcaa accgggagat agctggttct
      241 ccccgaaagc tatttaggta gcgcctcgtg aattcatctc cgggggtaga gcactgtttc
      301 ggcaaggggg tcatcccgac ttaccaaccc gatgcaaact gcgaataccg gagaatgtta
      361 tcacgggaga cacacggcgg gtgctaacgt ccgtcgtgaa gagggaaaca acccagaccg
      421 ccagctaagg tcccaaagtc atggttaagt gggaaacgat gtgggaaggc ccagacagcc
      481 aggatgttgg cttagaagca gccatcattt aaagaaagcg taatagctca ctggtcgagt
      541 cggcctgcgc ggaagatgta acggggctaa accatgcacc gaagctgcgg cagcgacact
      601 aagtgttgtt gggtagggga gcgttctgta agcctgtgaa ggtgtactgt gaggtatgct
      661 ggaggtatca gaagtgcgaa tgctgacata agtaacgata aagcgggtga aaagcccgct
      721 cgccggaaga ccaagggttc ctgtccaacg ttaatcgggg cagggtgagt cgacccctaa
      781 ggcgaggccg aaaggcgtag tcgatgggaa acaggttaat attcctgtac ttggtgttac
      841 tgcgaagggg ggacggagaa ggctatgttg gccgggcgac ggttgtcccg gtttaagcgt
      901 gtaggctggt tttccaggca aatccggaaa atcaaggctg aggcgtgatg acgaggcact
      961 acggtgctga agcaacaaat gccctgcttc caggaaaagc ctctaagcat caggtaacat
     1021 caaatcgtac cccaaaccga cacaggtggt caggtagaga ataccaaggc gcttgagaga
     1081 actcgggtga aggaactagg caaaatggtg ccgtaacttc gggagaaggc acgctgatat
     1141 gtaggtgaag cgacttgctc gtggagctga aatcagtcga agataccagc tggctgcaac
     1201 tgtttattaa aaacacagca ctgtgcaaac acgaaagtgg acgtatacgg tgtgacgcct
     1261 gcccggtgcc ggaaggttaa ttgatggggt tagcgcaagc gaagctcttg atcgaagccc
     1321 cggtaaacgg cggccgtaac tataacggtc ctaaggtagc gaaattcctt gtcgggtaag
     1381 ttccgacctg cacgaatggc gtaatgatgg ccaggctgtc tccacccgag actcagtgaa
     1441 attgaactcg ctgtgaagat gcagtgtacc cgcggcaaga cggaaagacc ccgtgaacct
     1501 ttactatagc ttgacactga acattgagcc ttgatgtgta ggataggtgg gaggctttga
     1561 agtgtggacg ccagtctgca tggagccgac cttgaaatac caccctttaa tgtttgatgt
     1621 tctaacgttg gcccctgatc ggggttgcgg acagtgtctg gtgggtagtt tgactggggc
     1681 ggtctcctcc taaagagtaa cggaggagca cgaaggttgg ctaatcctgg tcggacatca
     1741 ggaggttagt gcaatggcat aagccagctt gactgcgagc gtgacggcgc gagcaggtgc
     1801 gaaagcaggt catagtgatc cggtggttct gaatggaagg gccatcgctc aacggataaa
     1861 aggtactccg gggataacag gctgataccg cccaagagtt catatcgacg gcggtgtttg
     1921 gcacctcgat gtcggctcat cacatcctgg ggctgaagta ggtcccaagg gtatggctgt
     1981 tcgccattta aagtggtacg cgagctgggt ttagaacgtc gtgagacagt tcggtcccta
     2041 tctgccgtgg gcgctggaga actgaggggg gctgctccta gtacgagagg accggagtgg
     2101 acgcatcact ggtgttcggg ttgtcatgcc aatggcactg cccggtagct aaatgcggaa
     2161 gagataagtg ctgaaagcat ctaagcacga aacttgcccc gagatgagtt ctccctgact
     2221 ccttgagagt cctgaaggaa cgttgaagac gacgacgttg ataggccggg tgtgtaag
//
LOCUS       DAZGQL010000248         2219 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.250, whole genome shotgun sequence.
ACCESSION   DAZGQL010000248 DAZGQL010000000
VERSION     DAZGQL010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2219
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.250"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>2219
                     /locus_tag="ACHKIR_005199"
     CDS             <1..>2219
                     /locus_tag="ACHKIR_005199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO9992783.1"
                     /translation="KAGISVGQYKAAMRTLPAQFTDIVTQLAGGQNPFLIMLQQGGQI
                     SDSFGGPLSLLTLLKEELLGIRDASESSEDSLSDTANALAENARNAGELGRFMSVARV
                     AAGGGVAVLAALAAAAWQAEQADRALLRSLTLTGGAAATTTAELWKMAGVISDEAGGG
                     IRQAAENLARLAESGKYTAGQLRIMGETSQRWLQTVGDDAGKVEKAFEGIAADPVKAL
                     ASLNQQYNFLSVSQLRHIDELERTKGKQAAVTEAMSLFADVMNARLEQLDKAATPVEK
                     IWDDVKTWTSDAWAWIGDHTLGALSLITDVVAGTVEQVKLLLVQGDLALAEFIQSAWE
                     TTKNVPGVGALFGELAEENRVFIEKTKRDELALRKSIAERDVRIRQGEMGYINRSRAT
                     GVSKGPGQQEAVSRLAEELTGKKHTSPKTRSAGEREEEQAREALLALEAELRTLEKHS
                     GANEKISRQRRDLWKAESQYAVLKEAATKRQLSGQEKSLLAHKDETLEYKRQLAELGD
                     KVEYQKRLNELAQQAVRFEEQQSAKQAAISAKARGLTDRQAQRESEEQRLRDVYGDNP
                     QALAQVTGALKQTWADEDMLRGDWLAGLKSGWGEWAESATDSFSQVKSAATQTFDGIA
                     QNMAAMLTGAEADWRGFTRSVLSMMTEILLKQAMVGIVGRIGSAIGGAFGGGASASSG
                     TAIEAAAANFHFATGGFTGTGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYA
                     "
BASE COUNT          525 a          522 c          756 g          416 t
ORIGIN      
        1 aaagccggga tatcagtcgg acagtataag gctgccatgc gcacactgcc cgcacagttc
       61 acggatattg tcactcagct tgccggtggt cagaatccct tccttatcat gctgcagcag
      121 ggggggcaga tcagcgattc attcggtgga ccgctcagcc tgcttaccct gctgaaggag
      181 gaacttctcg ggatcaggga tgcctctgaa tcatcagagg attcgctgtc agatacggca
      241 aatgcactgg ctgaaaatgc ccggaatgcc ggtgaactgg gacgatttat gtcggtggcc
      301 cgtgtggcgg caggtggcgg ggttgccgta ctggccgcgc ttgctgctgc cgcctggcag
      361 gcagagcagg ctgaccgggc cttattgcgt tcactgaccc tgaccggagg ggcggctgcc
      421 accacaacgg cagaattgtg gaaaatggcc ggggtgatca gcgatgaagc cggtggtggt
      481 atcagacagg cggcagaaaa tctggcccgt ctggcagaaa gcgggaaata taccgccggg
      541 cagctacgga tcatggggga aacctctcag agatggctgc agacggtggg ggacgatgcc
      601 gggaaggtgg aaaaagcctt tgaagggatt gcagcagatc cggtgaaggc gctggcctcc
      661 ctgaatcagc agtataactt cctgagcgtt tcccagttac gccatattga tgagcttgag
      721 cgcacgaaag gtaaacaggc tgcggtgacg gaggcgatgt ccctgtttgc ggatgtcatg
      781 aatgcacgtc tggagcaact tgataaagcg gccacgccgg tggaaaaaat ctgggacgat
      841 gttaaaacct ggacttctga cgcatgggca tggataggtg atcatacact gggggcactc
      901 agtctgatca ctgacgtggt ggcaggaacc gttgaacagg tgaagctgct gcttgtgcag
      961 ggggatctgg cgctggctga atttattcag tcagcctggg aaacgacaaa gaatgtgccc
     1021 ggcgttggtg cgttgtttgg tgaactggca gaagagaacc gcgtatttat tgagaaaaca
     1081 aaacgcgatg aactggcgct gagaaaatcc attgcggaac gggatgtgcg tatacgccag
     1141 ggggaaatgg ggtacatcaa ccgttcgcgt gcaacaggcg tcagcaaagg tccggggcag
     1201 caggaagccg tcagccgtct ggctgaagag ctgacaggta aaaagcatac atcaccgaaa
     1261 acgcgctctg ccggggagag ggaagaggag caggcaagag aggctctgct tgcccttgaa
     1321 gctgagctca ggacgctgga aaaacacagc ggtgcgaatg agaaaatcag ccggcaacgc
     1381 cgtgatttat ggaaggcgga aagtcagtat gcggtcctga aagaggctgc cacgaaacga
     1441 cagttatccg ggcaggaaaa atccctgctg gcgcataaag acgagacgct ggagtacaaa
     1501 cgccagctgg ctgagctggg cgacaaggtt gaataccaga aacgcctgaa tgagctggca
     1561 cagcaggcgg tgcggtttga agagcagcag agcgcgaagc aggccgccat cagcgcaaaa
     1621 gcccgtggtc tcaccgaccg tcaggcacag cgggagtctg aagagcagcg tcttcgtgac
     1681 gtgtacggcg ataatccgca ggcgctggca caggtcaccg gggcactgaa acagacatgg
     1741 gcggatgaag acatgctgcg cggtgactgg ctggccgggc tgaagtccgg ctggggggag
     1801 tgggcggaaa gtgcgacgga cagtttttcg caggttaaaa gtgcggccac gcagaccttt
     1861 gatggtattg cacagaatat ggcggcgatg ctgaccggtg cagaggcaga ctggcgggga
     1921 ttcacccgtt cggtgctgtc catgatgaca gaaatcctgc ttaaacaggc catggtggga
     1981 attgtcgggc gtatcggcag cgccattggt ggtgctttcg gtggtggtgc atctgcttcc
     2041 tcggggacgg ccattgaggc tgcggcggcg aacttccatt tcgcgaccgg aggatttacg
     2101 gggacgggcg gcaaatatga gcctgcgggg atagttcacc gcggggagtt tgttttcacg
     2161 aaagaggcaa ccagccggat aggtgtgggg aatctttacc gtctgatgcg cggctatgc
//
LOCUS       DAZGQL010000249         2133 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.251, whole genome shotgun sequence.
ACCESSION   DAZGQL010000249 DAZGQL010000000
VERSION     DAZGQL010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.251"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..1540
                     /locus_tag="ACHKIR_005200"
     CDS             <1..1540
                     /locus_tag="ACHKIR_005200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992784.1"
                     /translation="FYHKDSTEDMLMNQLKSMNINKLLLDVDNPRFPTSAENQRDAIA
                     KMLDLQYDRIYRLAKDIASKGLDPSENILVYPSEEESGFFVVAEGNRRVTALKLLHSP
                     RLAPTEKARKAFEKLKITQLKDINVIDNCVLFDDDEYEHWVNLKHTGQNAGVGRVEWT
                     ATEKARHLARMGKQSFGNQILTFIEFNTDLYKEIIAKKRLLKITNITRLFGDLKVRDY
                     FNLKSINGVLYSYQPYERFCAQLKNILDVMIEEDDKGKACFTVNRIRSQDDRVTFIIE
                     QKIKPSESLLDKPWSLLEPKSFLTEKKHDSIDKNDASVSVKKVGLSEGDALKNTGVDK
                     QEDDYSRHVGTEESKSKVSGTEQNKNKGKTPPKVDRNVLVPSYVKFNFRGHKKCSRIF
                     NELKSHLTFDNTPNAISILLRIFIDLSVSTFVEENKLQFKEPHRTPGLHDKVKMCANF
                     LRENKKMTGSQCTAVITFSSQITKHDGSLQQYVHNPHLIPSKEAVNTEWDNFELLLSL
                     IWSE"
     gene            1610..>2133
                     /locus_tag="ACHKIR_005201"
     CDS             1610..>2133
                     /locus_tag="ACHKIR_005201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000675356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HFO9992785.1"
                     /translation="MKFYTPLRYPGGKGKLSYFLKDVIEKNSLNDGAYAEPYAGGAGV
                     ALELLLEEYVRKIYINDADFAVYSFWSSVVSDTDELCRLISRAKIDMDEWSFHRYVIS
                     NPSEFTKLEVGFAAFFLNRTNRSGILKAGVIGGKSQNGKWKMDVRFNRADLISRIEKI
                     ANYNQRIVVTNLDA"
BASE COUNT          718 a          308 c          445 g          662 t
ORIGIN      
        1 tttttatcat aaagattcaa cagaggatat gttgatgaat caactaaaat caatgaatat
       61 caataagtta cttttggatg ttgataaccc tcgtttcccg acgtcagcag aaaatcagcg
      121 tgatgctatt gccaagatgc ttgacttgca gtacgaccgc atttatcgct tggctaaaga
      181 tattgcttcg aaaggtttag atccgtctga aaacatatta gtttatccta gtgaagagga
      241 atctgggttt ttcgtcgttg ctgaaggtaa tcgtcgagtt acagccctta aacttttaca
      301 ctctcctaga cttgcaccaa cagaaaaagc acggaaagct tttgaaaaac ttaagattac
      361 tcagcttaaa gatattaatg ttatagataa ctgcgttctt tttgacgatg atgaatatga
      421 gcattgggtc aatttaaagc atactggtca aaatgctgga gttggaagag ttgaatggac
      481 tgcaacagaa aaagcacgac atctggctag gatgggtaag cagtcatttg gtaatcaaat
      541 acttacattt atagagttca atactgattt atataaagag ataattgcta aaaaaagact
      601 tttaaaaatc acaaacatca cgcgtctttt tggtgattta aaggtgaggg attattttaa
      661 tttaaaaagc attaatggag ttctatactc atatcagcct tatgaacgtt tttgtgcaca
      721 attaaaaaat attttagatg taatgattga agaggatgat aaaggtaagg cttgttttac
      781 tgttaatagg attcgtagcc aagatgacag ggttacattt attattgaac aaaaaataaa
      841 accttcagaa tctcttttag ataagccgtg gagtttactg gagcctaaat cttttcttac
      901 agagaaaaaa catgattcta ttgacaaaaa tgatgcatct gtgagtgtaa agaaagtagg
      961 tctttcagaa ggtgatgcct taaagaatac aggtgtggat aaacaggaag atgattattc
     1021 tagacatgta ggcacagaag agagtaaatc taaagtttcg ggtacggaac agaataaaaa
     1081 caagggaaaa actcctccta aagtagatcg taatgtgttg gtgccatctt atgtgaaatt
     1141 taattttaga gggcataaga aatgctctag aatatttaat gagcttaagt cgcatctgac
     1201 atttgataac acccctaatg ctatctcaat attgttgaga atatttatag atctctctgt
     1261 aagcactttt gttgaagaaa acaagttaca atttaaggaa ccacatagaa ctccagggct
     1321 tcatgataag gttaaaatgt gtgctaattt ccttcgggaa aataaaaaga tgacggggag
     1381 tcaatgtacg gctgtaataa ctttctcgag ccaaataact aaacatgatg gttcattgca
     1441 gcagtatgta cataacccac acttaattcc ctcaaaagag gctgtcaaca ctgagtggga
     1501 taactttgag ttgttattaa gccttatttg gagtgagtga gatttgtcta catctggaca
     1561 tggatgtcca gaaactgata aactacaaaa tcaggaatga gagggattta tgaaatttta
     1621 tactccatta agataccccg gcgggaaagg taagttatct tactttttaa aagatgttat
     1681 tgaaaaaaac tcgttgaatg atggagcata tgcagaacct tacgctggtg gtgcaggggt
     1741 ggctttagaa ttattattgg aagagtatgt ccgtaaaatc tatataaatg atgcagattt
     1801 tgctgtttat tctttttggt catctgttgt tagtgatact gatgagcttt gccgacttat
     1861 tagccgagca aagatagata tggatgaatg gtcttttcat cgctatgtta tttcgaatcc
     1921 ttctgagttt actaagcttg aggttggctt tgctgcgttt ttcttaaata gaacaaatcg
     1981 ttcaggtatt ctcaaggcgg gggttatagg cgggaaatca caaaatggaa aatggaaaat
     2041 ggatgttcgc tttaacaggg cggatttaat ttcaagaatt gagaagatag caaattataa
     2101 tcaaagaata gtagtaacta atctggatgc ttt
//
LOCUS       DAZGQL010000250         2119 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.252, whole genome shotgun sequence.
ACCESSION   DAZGQL010000250 DAZGQL010000000
VERSION     DAZGQL010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.252"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(15..905)
                     /locus_tag="ACHKIR_005202"
     CDS             complement(15..905)
                     /locus_tag="ACHKIR_005202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000236948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HFO9992786.1"
                     /translation="MVISENTVGTNDIPDSNHDILGPLMPARGFFFIVIDEPGKDGFL
                     VRKIYASPNSPYLTKMNLDPLRLLSETEPERYGLMPKDYSAPTTVAEHVMGNNKSSLI
                     STSSIFPDGSPRFEGKTIFIDVHKAKNAGLKLISTAEIITALEEYKKQYPHLAKRVEK
                     ISGYVDDIDKEVLIEGRKVPAQTIFTPESLKTTQNIVKAGRVVQVFSIFFTAYDLEQA
                     TEKSFETKSIKPIGAEAIRQIGGWGGAVAGAKIGTVIGASLGVTTGPGAVATGIIGGI
                     VFGAAGYFGADWVADYIDEN"
     gene            complement(899..>2119)
                     /locus_tag="ACHKIR_005203"
     CDS             complement(899..>2119)
                     /locus_tag="ACHKIR_005203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2345 domain-containing protein"
                     /protein_id="HFO9992787.1"
                     /translation="RQSRPYAGDTYGLHLPLLAGTEVSIAFEEGNPDRPYIAGVKHDS
                     AHTDHVTIQNYKRNVLRTPANNKIRLDDERGKEHIKVSTEYGGKSQLNLGHLVDAGKQ
                     QRGEGFELRTDMWGVVRAKKGIFISADAQDNAQGQVREMAPAMAILNGAQSQMKSLST
                     DAQTANADPADLSSQIALLQQSVKDLNQAAILLSAPKGVAIASGEHLQLAAAKNVIAN
                     AGNNADVGAVKNMFIGVGQALSVFVRKAGIKLFANKGAISVQAQNDLMELLAQKSIEI
                     TSTEDEIKITAKKKITLNGGGSYIRLDACGIEAGTQGEYNVKAGYYGRKPKAKLTPEL
                     MAFPVISAKFDEQVRVIDDDGKFIANVPYYIEDSIGNIYKGFTDSAGCCERIFTEEAD
                     NLNVLLGIKALEKW"
BASE COUNT          479 a          571 c          437 g          632 t
ORIGIN      
        1 tgtgctctat ccaattagtt ttcatctata taatccgcta cccaatctgc accaaaataa
       61 ccagctgcac caaaaacaat cccgccaata atcccggtag ccactgctcc tggcccagta
      121 gtaacaccca gggacgcgcc tatcactgtt ccaattttag caccagctac tgctcctccc
      181 catcctccaa tttgccttat tgcttctgca cctataggtt ttatgctttt tgtttcaaac
      241 gatttttcag ttgcctgttc taaatcatat gcagtgaaaa aaatactaaa aacttgtacg
      301 acacgcccgg ccttaacaat gttttgagtc gtttttagag actctggtgt aaaaattgtt
      361 tgtgctggta cttttctacc ttcgatcaag acctctttat caatatcatc aacatagcct
      421 gaaatttttt caacacgctt agccaaatga gggtattgtt tcttatactc ctcaagagca
      481 gtaataatct ctgcggtcga gattaacttc aacccggcat tttttgcttt gtgaacatca
      541 ataaatatgg ttttcccctc aaatctcgga gagccatcag gaaatataga gcttgtagaa
      601 atcaacgagg atttgttgtt ccccataaca tgttctgcta cagtagtggg tgctgaataa
      661 tcttttggca tcaacccata gcgttcaggc tctgtttctg ataataatct aagaggatcc
      721 agattcatct ttgtcaggta ggggctattt ggtgaagcat atatttttct gacaagaaag
      781 ccatcttttc ccggctcatc gataactata aaaaagaaac ctcttgcagg cattaagggt
      841 cctaatatat cgtgatttga atcaggtata tcatttgttc ctactgtatt ctcagatatc
      901 accatttttc cagcgcctta ataccaagaa gtacgtttag gttatcagct tcttccgtga
      961 aaattctttc acagcacccg gcagaatcag taaacccctt atagatattc cctatgctgt
     1021 cttctatata gtaaggtaca ttagcaatga attttccgtc atcatcaatt actctaactt
     1081 gctcatcaaa tttcgcagat attacaggaa acgccatcag ctccggcgtc agcttcgcct
     1141 ttggtttacg cccgtaatat ccggccttaa cgttatattc tccctgcgtt cccgcttcta
     1201 tcccacaggc atccagccgg atgtagctgc caccgccgtt aagggtgatt ttcttcttcg
     1261 cggtgatctt gatctcatct tccgtactgg tgatctctat cgatttctgt gccaacagtt
     1321 ccatcagatc gttctgtgcc tgtaccgaga tcgccccctt gttggcaaac agcttgatgc
     1381 ccgctttacg cacaaacacg cttagcgcct gtcccacgcc gataaacata tttttcaccg
     1441 cgccgacatc ggcgttgttg cctgcgttgg ctatcacatt ctttgccgcc gccagttgca
     1501 ggtgctcgcc gctggcgatc gccacgccct tgggtgcgct tagcaggatc gcagcctggt
     1561 tcagatcttt tacgctctgt tgcaacaacg caatctgcga tgacagatct gccggatcgg
     1621 cattcgccgt ctgcgcatca gtggacagag acttcatctg tgactgtgcg ccattcagta
     1681 tcgccattgc aggagccatt tcgcgcacct gcccctgcgc attatcctgc gcatcggcgc
     1741 tgataaagat gcctttcttt gcccgcacca cgccccacat atcggtacgc agttcaaagc
     1801 cttcgccgcg ttgctgtttc ccggcatcca ccagatgacc gaggtttagc tggctcttgc
     1861 cgccgtactc ggtactgacc ttgatatgtt ctttgccgcg ctcatcgtcg aggcggattt
     1921 tgttgttcgc cggggttctg aggacgttac gcttatagtt ctgaatggtc acatggtcag
     1981 tgtgtgccga gtcgtgtttt acgcctgcaa tataaggtcg gtccgggttg ccttcctcaa
     2041 aggcgatact gacttccgtt cccgccagca gcggcaggtg caggccgtag gtgtctcccg
     2101 catacgggcg cgactggcg
//
LOCUS       DAZGQL010000251         2113 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.253, whole genome shotgun sequence.
ACCESSION   DAZGQL010000251 DAZGQL010000000
VERSION     DAZGQL010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.253"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..291
                     /locus_tag="ACHKIR_005204"
     CDS             <1..291
                     /locus_tag="ACHKIR_005204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HFO9992788.1"
                     /translation="MVALTRMVLGSPRVVLLDEPTTGLDLQTESVVLKGLSHWSRGRT
                     MVVVTHRLQVLNIVDRIVVMNEGRVMMDGPRDEILKKLSGKQQSGESRAITS"
     gene            351..1598
                     /locus_tag="ACHKIR_005205"
     CDS             351..1598
                     /locus_tag="ACHKIR_005205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HFO9992789.1"
                     /translation="MTEKTERNKASSEVVRQSDLALLTDVQAALQQEKHHALFLMVIF
                     LLVLVVTFVIWAWNSPLDEVTRGQGSIIPGSREQVIQTLDPGILKSLEVREGDIVEKG
                     QVLLTLDDTRSSAMLRESEARVNNLEAVRARLRAEAYSESLTFPDNVPEDLRERESTV
                     YFLRKTELAQSIAGLKQSKALLDREIAMTRPIVREGAMSEVELLRMQRQSAELQLQMD
                     EKQNKYLTEAGAELVKTEAELAQAKENMAGRADPVERSRLRAPLRGIVKNIRVNTLGG
                     VVSAGQDIMEIIPLEDQLLIEAYINPRDVAYVRTGMPALVKLTAYDYAIYGGLDGVVT
                     LVSPDTLRDQKRPGDLKLDPNEAYYRVLVTTSNNYLTDRNGKRLPVIPGMIASVDIKT
                     GRKTVFQYLIKPITRMKQALQER"
     gene            1884..>2113
                     /locus_tag="ACHKIR_005206"
     CDS             1884..>2113
                     /locus_tag="ACHKIR_005206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992790.1"
                     /translation="MKMIFTGKVSGEKTVLTAGARHTVKAQAGEQYGLVDEVTGLVPD
                     GVEADRSGDDLILRKKEDDTEIRIEGFWEECQP"
BASE COUNT          606 a          398 c          619 g          490 t
ORIGIN      
        1 atggttgccc tgacgagaat ggtactgggt tctccccggg tggtactgct tgatgagccc
       61 acgacaggtc tggacctgca gactgagtct gttgttctga aaggtctgag ccactggagc
      121 cgtggacgca caatggtggt ggtgacacat cgtctgcagg ttctgaatat tgttgaccgt
      181 attgtcgtga tgaatgaagg gcgggtaatg atggacggtc cccgtgatga aatactgaaa
      241 aaactgtcag ggaaacagca gagtggggag agcagagcga taacttcatg agcgtcaacc
      301 ggataagaaa aaagagcaga tatgtttaac agtttgtgga ccgcagggat atgacagaaa
      361 aaacggaacg gaataaagcg tcgtcagagg tggtcaggca gtcagacctg gcgctgttga
      421 cagatgtaca ggcagccctt cagcaggaaa agcatcatgc ccttttcctg atggtgattt
      481 ttctgctggt actggtagtg acgtttgtta tctgggcatg gaacagtcct ctggatgaag
      541 tgacccgagg tcaggggagt attattcccg gcagcaggga gcaggttata cagacccttg
      601 acccgggtat actgaaatca cttgaggtca gggaaggcga tattgttgag aaaggtcagg
      661 tactgctgac actggatgat acccggagct ctgccatgct gagggaaagt gaagcccggg
      721 tgaataacct ggaggccgtc agggcaagac tccgtgcaga agcttattcc gaatcactga
      781 catttccgga taacgttcct gaggatttgc gggagcggga aagtacggtc tattttctcc
      841 ggaagacgga gctggcgcag agcattgccg gtctgaagca gagtaaggcg ctgctggaca
      901 gggaaatagc aatgacgcgg ccaattgtgc gtgaaggggc aatgtcggag gtcgaactgt
      961 tacgtatgca gcgtcagtca gcggagctgc agttacagat ggatgagaaa cagaataaat
     1021 acctgacgga agccggtgct gaactggtga agacagaagc agagcttgct caggcaaaag
     1081 agaatatggc tggcagagcc gacccggttg agcgttcacg tctcagggcc cccctgagag
     1141 gtatcgtaaa aaatatacgg gtaaacacac tgggaggggt ggtttctgca ggacaggaca
     1201 taatggaaat tattccgctg gaagaccagt tgctgattga agcttatata aatccacggg
     1261 atgttgccta tgtcagaaca gggatgccgg cacttgtaaa actgacggct tatgactatg
     1321 ccatttatgg tggtcttgac ggcgtggtga cactggtaag tccggatact ttacgtgacc
     1381 agaaacggcc gggagattta aaactggacc cgaatgaagc ttattaccgg gtactggtta
     1441 ccaccagtaa taattatctg acagacagga acggaaaaag attgccagtt attcctggca
     1501 tgatagccag cgtggatata aaaaccgggc gtaaaacggt atttcagtat ctgattaaac
     1561 cgataaccag aatgaagcag gcacttcagg aaagataata aaaaggtctt ccggggaaag
     1621 tattttaaat caattttaaa cgggcagaat gatttcactt tattctgccc gggtcattgc
     1681 gcatgcctgt atgtgatttg gggctgaaga taatataaat gttatatctg tcaggggaaa
     1741 ataaatctga aaaaagcgga atgattaagg aggtccggtc agagaaataa attatctgta
     1801 atctgattcg tttttattta atgactccct ttagcggata aaacataaga gcatactggt
     1861 tttattaacc tgaggatact tatatgaaaa tgattttcac cggcaaggtc agtggagaaa
     1921 aaacagtact gacagccgga gcccgtcata cagtaaaggc acaggccggg gaacagtatg
     1981 gactggttga tgaggtgaca ggtctggtcc cggatggtgt ggaagcggac cgttctggtg
     2041 atgatttaat tctgcgtaaa aaggaagatg atacagaaat ccggattgag ggattctggg
     2101 aagaatgcca gcc
//
LOCUS       DAZGQL010000252         2112 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.254, whole genome shotgun sequence.
ACCESSION   DAZGQL010000252 DAZGQL010000000
VERSION     DAZGQL010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.254"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..534
                     /locus_tag="ACHKIR_005207"
     CDS             <1..534
                     /locus_tag="ACHKIR_005207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFO9992791.1"
                     /translation="IGLKGGWNFYQYPLNPVQYIDSMGLASKYGHLNNGGYGARPNKP
                     PTPDPSKLPDIAKQLRLPYPIDQASSAPNVFKTFFRALSPYDYTLYCRKWVKPNLTCT
                     PQDDPQYPGMDTKTASDYLPQTNWPTTQLPPGYTCAEPYLFPDINKPDGPATAGIDDL
                     GEILAKMKQRTSRGIRK"
     gene            531..1100
                     /locus_tag="ACHKIR_005208"
     CDS             531..1100
                     /locus_tag="ACHKIR_005208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992792.1"
                     /translation="MKRILFFLLMIFVSFGVIADCETQAKDQDCFTIFTKGTIFSAFP
                     VLNNKTIWRWYQNEDIGEYYWQTELGICKNNKFTPSGARLLIRVGSLRLNENHATKGT
                     LQELLNTAEKTAFLGDRFRSYIRAGIYQKKSSDPAQLLAVLDNSIMVKYFKDEKPTYA
                     RMTAHLPNKDESYECLTKVQHELLRSEEK"
     gene            1344..1571
                     /locus_tag="ACHKIR_005209"
     CDS             1344..1571
                     /locus_tag="ACHKIR_005209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000404859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTF2 fold immunity protein"
                     /protein_id="HFO9992793.1"
                     /translation="MEILVNSREMALELSYVYIKYVYGKEKAEFQKPYSITDDNNCWK
                     IEGKQPKTLGGNFTILIAKKDGQVLNVIHTK"
     gene            complement(1933..>2112)
                     /locus_tag="ACHKIR_005210"
     CDS             complement(1933..>2112)
                     /locus_tag="ACHKIR_005210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992794.1"
                     /translation="YSKNTQAFRTLYQPHLDCFPFPNTAARGVIKVRNFQQYGVLLFT
                     AVMHQPVLAIVVITV"
BASE COUNT          730 a          372 c          435 g          575 t
ORIGIN      
        1 attgggctga aggggggatg gaatttttat cagtatccgt tgaatccggt tcagtatata
       61 gattcaatgg gactggcatc aaaatatgga cacttaaata atggcggata tggagcgaga
      121 cccaacaaac cgcctacgcc cgatccaagt aaattgccag acatagcgaa acaattaaga
      181 ctgccatatc ctattgacca ggccagtagt gcgcctaatg ttttcaaaac attcttcaga
      241 gcattaagcc cttacgacta cacactgtat tgcaggaagt gggtaaaacc aaatctgact
      301 tgtacgccac aggatgatcc ccagtatcca gggatggata caaagacagc aagtgattac
      361 ctgccacaga caaattggcc aacaactcaa ttaccaccag gatatacttg tgcagaaccc
      421 tatttattcc cagacattaa taaacccgat gggccagcaa cagcagggat agatgatttg
      481 ggtgaaattt tagctaaaat gaaacagaga acatcgagag gaataagaaa atgaaaagaa
      541 ttttgttctt tttgctgatg atatttgtta gttttggtgt tatagctgat tgcgaaacac
      601 aagctaaaga tcaagattgc tttaccattt tcactaaggg tactattttt tccgcgtttc
      661 ctgtcttaaa taataaaacc atatggagat ggtatcagaa tgaggacata ggtgagtatt
      721 attggcaaac agaacttggt atatgtaaaa ataacaaatt cacaccaagc ggggcgagac
      781 tcttaattcg cgtcggatca ctacgcctta atgaaaatca tgcaaccaaa ggaacgttgc
      841 aggagttgct aaatacagca gaaaaaacgg cgtttctcgg cgatcggttt agaagttata
      901 taagagcggg tatctatcaa aaaaagagca gtgatccagc tcagttgttg gcagtccttg
      961 ataattcaat catggttaaa tattttaaag atgagaagcc aacttatgct cgaatgacag
     1021 cacatctccc gaacaaagat gagtcttacg aatgtttaac aaaagtacag catgaactgc
     1081 ttcgtagtga agaaaaataa aatctcttcc tgagaaaata aattaactat tcgaaaaaat
     1141 atgttcttaa tgggtatcca ggtacaccag aaggtcgatt tggaaaaatg gattccgatg
     1201 gtaagaatat catatattct gaatgtaatg ttttacccac aacttttgag attcgatagg
     1261 tgtagagcat gagaaactca ctaatattac ttattatgat attgtttaca atatcacact
     1321 cagtatttgg aaaagaaaat tcagtggaaa ttttagtaaa tagtcgtgaa atggctttgg
     1381 aattatcata tgtttacatt aaatatgtat acgggaaaga aaaagcagaa tttcagaaac
     1441 catatagtat tacagatgac aataattgtt ggaaaatcga gggaaaacaa ccgaagacgt
     1501 taggaggaaa ttttacaata ctaatagcga aaaaagatgg gcaagtgttg aatgtaatac
     1561 acacaaaata atccactgta cagtcgattc aaatatttta ccaaggtgtg ttttgtaaca
     1621 attttgtgta caatatgact ctacatatcg acgaaaaaac cacttgattt gacaggaaca
     1681 cattcaaaaa aatttcttta ttcatttcag gagcgatttt catctgttac ttgaagtatc
     1741 tgtgagatta agtgactccc cctaacgtcg tactaaatag taattactgt ccataccggg
     1801 agcagtattc ggcgctcccc acatgcagca gaataaccgc atcagcgaag atgtattgta
     1861 tttttactat cacaatgtgt acggacggtt tatggaaaag aatgaaaatt agatacgcga
     1921 tggggcggca ggttacacgg ttatcactac gatagccaat actggctggt gcattaccgc
     1981 cgtgaacaat aggacgccgt actgctggaa atttcttacc tttatgactc cccgcgccgc
     2041 cgtattggga aacgggaagc aatcaagatg aggctgatat aatgtgcgga atgcctgagt
     2101 atttttagaa ta
//
LOCUS       DAZGQL010000253         2109 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.255, whole genome shotgun sequence.
ACCESSION   DAZGQL010000253 DAZGQL010000000
VERSION     DAZGQL010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.255"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..449)
                     /locus_tag="ACHKIR_005211"
     CDS             complement(<1..449)
                     /locus_tag="ACHKIR_005211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFO9992795.1"
                     /translation="MRGSVIIPVALHALVAVSTLLWLLWYFIPAGNVFNGLATLLILL
                     LAGWSVFKNSRSEKQKLTSDVTLTDAEPALSDTRGPVVLVCGDMPEALFQDGPLRKTA
                     RGWWLRVGDVSRLTDVVRSIQTQFPRQVGQLSVMYRCLPDCHHDEAVL"
     gene            complement(449..1138)
                     /gene="tssL"
                     /locus_tag="ACHKIR_005212"
     CDS             complement(449..1138)
                     /gene="tssL"
                     /locus_tag="ACHKIR_005212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssL, short
                     form"
                     /protein_id="HFO9992796.1"
                     /translation="MRQDIDIDQLMMETWLTVTMLKKGALTPDGTALYNKCVKQVESV
                     REALERAGYDDASVEHITYAQCALLDETVMSRKPEKSGEGGEPDVDEGQAAWRKVPLQ
                     ARFFGSLRAGEALWDRIAEVLRQPSPDPAVLTCFHRVTALGFQGVYGLKAVSQSQRDE
                     VMKALSERVSLPDGGLSLVVYRMEKHRWRLIRSVWFWTVLAVILTGVIWWGGYLWLQS
                     LLSSQIPELHG"
     gene            complement(1135..>2109)
                     /gene="tssK"
                     /locus_tag="ACHKIR_005213"
     CDS             complement(1135..>2109)
                     /gene="tssK"
                     /locus_tag="ACHKIR_005213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020884777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HFO9992797.1"
                     /translation="VRYREEWVTLQDAFGSEEEPIAVARFNFTIRFAHENNDSWKVCP
                     VARLIRDGQNAWRQDPSFIPPVALFGASPVLRERLVLLNRQLRSRRQRLMAMRRESNE
                     RLADFAVADVSLFWLLNALNSHASVLTEYERFPSRPPEQVWAELARLAGSMLTFSLDH
                     DPDAIPGYDHKDPAGAFLPLFDLITGLLEACLPSRVIALEMSRPDGQTWKASLHDIRL
                     REEADLWLSVRSDTPAWQVAEKFPALCLAGSPDNVSEIYGAALRGIPLIPVSRVPAAL
                     PVRMENQYFALDMESPAAHEMLEQGVCMFYVPELLGALELELFAVLRS"
BASE COUNT          538 a          630 c          537 g          404 t
ORIGIN      
        1 agaaccgctt catcatgatg acagtctggc aggcagcggt acatcacaga gagctgaccg
       61 acctggcgcg ggaactgcgt ctgtatgctg cggacaacgt cagttaagcg actgacgtca
      121 ccgacccgca gccaccatcc ccgggctgtt ttacgcagcg gaccgtcctg aaacaacgct
      181 tccggcatgt ctccacatac cagtacaaca gggccccggg tgtcggataa tgccggttca
      241 gcatccgtca gggtaacgtc agatgtgagc ttttgctttt cactccggct gtttttaaaa
      301 acactccagc ctgccagaag aaggatcagc agagtagcca gaccgttaaa aacgttgcct
      361 gccgggatga aataccacag caaccacagc aacgtggaaa cggccaccag ggcatgcagg
      421 gctaccggaa tgatgacaga tccacgcatc agccatgcaa ctccggtatt tgtgatgaca
      481 aaagagactg aagccagagg tacccccccc accagataac gccggtaaga atgacggcga
      541 gcactgtcca gaaccagact gagcgtatca gacgccagcg atgtttctcc atccggtaaa
      601 caaccaggga cagaccacca tcaggcagag aaacacgttc agaaagggct ttcatgacct
      661 catcacgctg tgactggctg actgctttca ggccgtacac cccctgaaaa cccagtgctg
      721 tcacccggtg aaagcaggtt aaaacggccg ggtccggtga aggctgacgc agaacctctg
      781 caatgcgatc ccacagtgct tcaccagccc gaagagaacc aaaaaagcgg gcctgaagcg
      841 gcactttacg ccaggccgcc tggccttcgt caacgtccgg ctccccgcct tccccgcttt
      901 tttcaggctt acggctcatc accgtttcgt ccaggagggc acattgtgca taggtaatat
      961 gctccacact ggcatcgtca taaccggcac gctccagcgc ttcccgcaca ctttcgacct
     1021 gtttaacgca tttgttgtaa agcgcagtgc catcgggcgt gagtgcacct tttttcagca
     1081 tggtgacggt aagccatgtc tccatcatca gctggtcgat atcaatatcc tgtctcatga
     1141 gcgcaatacc gcaaacagtt caagttccag ggcaccaagt aactcaggca catagaacat
     1201 acacactccc tgctccagca tttcatgtgc agcgggactt tccatatcaa gtgcgaagta
     1261 ctggttttcc atacgtacag gcaacgcagc cggaaccctg ctcaccggga tcaaagggat
     1321 ccccctgagt gccgcgccat agatctcact gacattatca ggtgaacccg ccagacagag
     1381 tgccggaaat ttctcagcaa cctgccaggc cggagtatcc gagcgcacag acagccagag
     1441 gtccgcctct tcgcgcaggc ggatatcgtg aaggctggcc ttccaggtct gtccatccgg
     1501 gcgggacatt tccagagcaa tgacccggga tggcagacag gcctccagca gacccgttat
     1561 caggtcgaac agcggaagaa acgcaccggc cggatccttg tggtcataac ccggtatggc
     1621 gtcaggatca tgatccaggg aaaaagtcag catactgcct gccagacgag cgagctccgc
     1681 ccacacctgt tcaggtggac gggaaggaaa acgttcgtat tccgtcagta cactggcgtg
     1741 ggagttcagc gcattcagca accagaaaag ggaaacatca gcgacagcaa aatcggcgag
     1801 tctttcgtta ctttcacgac gcatcgccat cagacgctgg cgtcgggagc gtaactgacg
     1861 attgagcaac accagacgtt cgcgcagaac cggacttgcc ccaaataacg ccaccggagg
     1921 aataaaagac ggatcctgtc gccacgcatt ctgaccatct ctgatcaatc ttgctaccgg
     1981 acagactttc catgaatcat tgttttcatg ggcaaaccgg atggtgaaat taaaccgggc
     2041 aacggctatg ggctcttctt cagagccaaa agcatcctgc agcgtgaccc actcttcccg
     2101 atagcgaac
//
LOCUS       DAZGQL010000254         2085 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.256, whole genome shotgun sequence.
ACCESSION   DAZGQL010000254 DAZGQL010000000
VERSION     DAZGQL010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.256"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(620..1711)
                     /locus_tag="ACHKIR_005214"
                     /pseudo
     CDS             complement(620..1711)
                     /locus_tag="ACHKIR_005214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627039.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
     gene            complement(1974..>2085)
                     /locus_tag="ACHKIR_005215"
     CDS             complement(1974..>2085)
                     /locus_tag="ACHKIR_005215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HFO9992798.1"
                     /translation="LVLQITLLGWIPATIWAVYALSQYKTEQKIREATGK"
BASE COUNT          634 a          487 c          381 g          583 t
ORIGIN      
        1 tattaaagat tctcaccatc attcgcagtg aataatgccg catctcatat tcatatcctg
       61 cagagaaaac ccctccgaag aggggcgagt gtagcgttaa tggagtgtta tgtagcatgg
      121 tgccgggtgc ctcccggtga acagactgtt cgtgatacca gtcagcaaca taggaaatta
      181 atggtatcac cccaccgcac agggggattc accatgcctg ccttttttag caaattcatc
      241 agaaaccgga caacatgctt atttatgaaa tgaggtgctt ttcacattta tataaaatca
      301 ttataacgat aatcctaaaa atcaatgtaa ttatcatctt cagtaacttt ctgtattaca
      361 ccatacggaa attgattttt gacggtaaag atacaagggg aagtatgtct gtaaggagta
      421 ccctgaataa tggacaggca tgcatcgtgg ctgttcccct gaaattatca gtacaaccag
      481 tcagctcata atccctgtct ctttttccgg ggagccctca gggggacttt cccttatccc
      541 cccagtttct ggagagacgt aaaaaagaat taatcatatg tcaggtatct gaatatgtcc
      601 cgtaattaat atatttacgt caggattttt tctgcccgcc aggagtatga acatcttccg
      661 ccgttgagtc tgtcatatcc atttttttct gtgccagtac ttcatatgat ttttcttttt
      721 ccagactctc ctgctgcatc ccgtgagccg gagcatatac aagctcagcc atcactggaa
      781 aatgatctga acccatatcc ggtaaacggc gtaaactttt aagcagaaaa tgctggctgt
      841 gaaatacatg gtcaagcggc catcgcagga aaaaataatg gctgtgaaac gtgctaaaaa
      901 aaccacgtcc ccggcgtgga tccagcagtc cacttatttt catgaataaa cgagtggtcg
      961 cagaccacgc aacatcattc agatcccccg ctaatattac aggtaattca gaatctgcaa
     1021 cacttttccc gaccattaaa agttctgagt cacgatcagt tgactcatca ctttcagtcg
     1081 ggctcggtgg aacaggatga agacaatgta atcttattct ctgaccttcc ggcatatgaa
     1141 ctatcatatg catggaggga atttccttgt ccactaaata ctgtatttct gcatcactta
     1201 aaatcatcct tgaatataca tgcatgccat ataaattttc tttcgggcat ttcagggagt
     1261 atggaaacac ttcatccaga ggtgatagtt tctgttccca ccataagttt gtttcaagaa
     1321 gaacaagtat atcaggtttt tctttattta taagttcaag caaaggcata tattttttat
     1381 tggtcataag gacattgaat accagtatac ttaaacccgg aaatccatca gagtccttta
     1441 tttactttac ctgcttcttg ccaattactg tataaggaat tatccatacc aactggtaag
     1501 cgacacaaat taaacttatt atcacaacaa acaactctgc aaatgagtca agaaaaacaa
     1561 cagacagaaa aacattcaaa gtacacagca aaagtatctg tagtcgggga aaatcccata
     1621 ccctgacaac ccatgatgta ttaccggaaa cagggataaa agttatgact gccagaagga
     1681 tagcagtaaa aataaaaaca caagttatca caaatcactc cttgttctga accggcacac
     1741 aaaactgtca tatacgtttc aaaagtaaaa acacactgct gccacaagat tcacgacgta
     1801 accgggcagc atatcctgat tacggacaat ccatgaaacc gcctcgccag aagcgtccat
     1861 cacatccggt ttttccctgt tttatattcc acgaaccatt ttattttcag gaatctccgg
     1921 gcctttatcc cgcatcatat acacagctat gacaacccgc tcttctgcag tcatcatttc
     1981 cccgtggctt cgcggatttt ctgttccgtc ttatactgcg aaagcgcata cacggcccat
     2041 attgttgccg gtatccagcc cagcagcgtt atctgtaaca ccaga
//
LOCUS       DAZGQL010000255         2010 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.257, whole genome shotgun sequence.
ACCESSION   DAZGQL010000255 DAZGQL010000000
VERSION     DAZGQL010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.257"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(31..>2010)
                     /locus_tag="ACHKIR_005216"
     CDS             complement(31..>2010)
                     /locus_tag="ACHKIR_005216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO9992799.1"
                     /translation="DDKGRLTGERQTVENPETGELLWQHETKQAYNEQGLANRVTPDS
                     LPPVEWLTYGSGYLAGMKLGGTPLLEYTRDRLHRETVRSFGSMAGNTAAYELTSTYTP
                     AGQLQSRHLNSLVFDCDYNWNDNGDLMRISGPRQTREYGYSATGRLESVRTLAPDLDI
                     RIPYATDPAGNRLPDPELHPDSTLTVWPDNRITEDAHYLYRYDEYGRLAEKTDRIPAG
                     VIRTDDERTHHYHYDSQHRLVFYTRIQHGEPLVESRYLYDPLGRRTGKRVWRRGRDLT
                     GWMSLSRKPEETWYGWDGDRLTTVQADTTRIQTVYQPGSFTPLIRIETDVTELEKAQR
                     RSLAEKLQQEGSEDGHGVVFPAELVRLLDRLEEEIRTDRVSSESRAWLAQCGLTVEQL
                     EKQVEPEYTPARKVHLYHCDHRGLPLALISEDGNTAWSGEYDEWGNLLNEENPHHLHQ
                     PYRLPGQQHDEESGLYYNRHRYYDPLQGRYITPDPIGLRGGWNMYQYPLNPIQVIDPM
                     GLDAIENMTSGGLIYAVSGVPGLIAANSITNSAYQFGYDMDAIVGGAHNGAADAMRHC
                     YLMCRMTKTFGSTIADVIGKNHEAAGDRQGQPAKERIMDLKNNTVGIACGDFSAKCSD
                     ACIEKYNTGQLFGLDGIKADNPIKAKQGSSDASNY"
BASE COUNT          353 a          638 c          514 g          505 t
ORIGIN      
        1 agggtaatta aaaaaatgac aagtttgcct ctaataattt gaagcatctg aactcccttg
       61 ctttgctttt attggattat ctgcttttat accatctaac ccgaagagtt gcccagtgtt
      121 atatttttca atacatgcat cgctacattt ggcagaaaaa tcgccacaag caataccgac
      181 agtgttattt ttaagatcca tgattctttc tttagctggc tgaccttgtc tatcccctgc
      241 cgcctcatga tttttaccta tcacgtcagc tattgttgat ccaaatgtct tagtcattcg
      301 acacatcaag taacaatgtc tcattgcatc ggcggcccca ttatgagctc cgccaacaat
      361 agcatccata tcataaccga actggtaagc actgttagta atgctgtttg cagcaatcaa
      421 tccaggtaca ccagatacgg cataaattag tccacctgat gtcatattct caatcgcatc
      481 taaccccatt gggtctatca cttgtatggg attcaacgga tactgataca tattccatcc
      541 acctctcaac ccaatcgggt ccggggtgat ataccgcccc tgcaacggat cgtagtaccg
      601 gtgacggtta tagtacagcc ccgactcctc atcatgctgc tgccctggca gacggtacgg
      661 ctggtgcagg tgatgcgggt tttcctcatt cagcaggttt ccccactcat catactcccc
      721 gctccacgcc gtattgccgt cttcgctgat aagcgccagc ggcagtcccc ggtggtcgca
      781 gtggtaaaga tgaacttttc gcgccggtgt gtattccggc tccacctgtt tttccagttg
      841 ctccaccgtc agcccgcact gcgcaagcca cgcccggctt tcactgctca cgcggtctgt
      901 ccggatttct tcctccagcc tgtccagcag ccgcaccagt tcagccggga acaccacgcc
      961 gtgtccgtcc tcgctccctt cctgctggag cttctccgcc aggctgcggc gctgcgcttt
     1021 ctccagttcg gtcacgtctg tctcgatgcg gatgagcggc gtgaagcttc ccggctggta
     1081 taccgtctgg atacgcgtgg tgtcggcctg caccgtcgtc agcctgtcac cgtcccagcc
     1141 gtaccacgtc tcctccggtt tacgcgacag cgacatccat cctgtcaggt cacgcccgcg
     1201 acgccacacc cgtttacccg tccggcggcc cagcgggtcg taaaggtagc ggctctcgac
     1261 cagtggctcg ccatgctgta tccgcgtata gaacaccagg cggtgctggc tgtcgtagtg
     1321 gtagtggtgg gtccgctcgt cgtccgtccg tatcacaccc gccgggatgc ggtccgtctt
     1381 ctccgccagc ctgccgtatt catcgtaacg gtacaggtag tgcgcgtctt cggtgattcg
     1441 gttatccggc cacactgtga gtgtactgtc cgggtgcagc tccgggtccg gcagccggtt
     1501 gcccgccggg tcggtggcat acgggatgcg gatgtccagg tccggtgcga gggtgcgcac
     1561 gctctccagc ctgcccgtgg cgctgtagcc gtattcccgc gtctgtcgcg ggccgctgat
     1621 gcgcatcagg tcgccgttgt cattccagtt atagtcacaa tcaaacacca ggctgttcag
     1681 gtgccggctc tgtaactggc ctgcgggggt gtaagtgctg gtcagttcat atgcggctgt
     1741 attgcctgcc atgctgccga agctgcgcac cgtctcccgg tgcaggcggt cgcgcgtgta
     1801 ctccagcagc ggcgtcccgc ccagcttcat gcccgccaga taaccgctgc cataggtcag
     1861 ccactccacc ggcggcaggc tgtccggcgt gacgcggttt gccagcccct gttcgttgta
     1921 tgcctgtttc gtctcatgct gccacagcag ttcccccgtc tccgggttct ccaccgtctg
     1981 gcgctccccg gtcagacggc ccttatcgtc
//
LOCUS       DAZGQL010000256         1991 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.258, whole genome shotgun sequence.
ACCESSION   DAZGQL010000256 DAZGQL010000000
VERSION     DAZGQL010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.258"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..766
                     /locus_tag="ACHKIR_005217"
     CDS             <1..766
                     /locus_tag="ACHKIR_005217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ArdC family protein"
                     /protein_id="HFO9992800.1"
                     /translation="LLWLAADEAGYVSNDWLTYRQAQQLGGHVRKGEVATLAVIYKDW
                     KKQAVDEHGNRLTDSEGEPLMEIVPVLKSNPLFNVGQCDGLPEHLMVKAEGSADALPA
                     DTFSERVLQVLNGSGVSVSSLPQDRAFYRPDLDRIVMPLSSQFDQVADYWTTLLHELV
                     HSTGHEKRLRRVGISTFTGRNSPEYAFEELIAEMGSAFLCAHLGIAGDVQHESYIDGW
                     LKVLKSDKKALFRACRQAREASEYLLTRRTSQECAA"
     gene            876..1310
                     /locus_tag="ACHKIR_005218"
     CDS             876..1310
                     /locus_tag="ACHKIR_005218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD29 domain-containing protein"
                     /protein_id="HFO9992801.1"
                     /translation="MNDNALVNVDDAVVARIRDVLAYTFPGQKFSVRKCDPSFPKVFN
                     NVWVSWLDGPTEAEVRSVTEGFENKDGLLSVLESRYFSDELVQECIDRLRKEYGQGNV
                     PLSVTVERYRKNELEKIETARFPGNISVAINEIGINTSKYRR"
     gene            1314..1628
                     /locus_tag="ACHKIR_005219"
     CDS             1314..1628
                     /locus_tag="ACHKIR_005219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992802.1"
                     /translation="MAGKCLVWFCNGISEPCLIAGNISELLADVTITSNYRGDVTSEI
                     RDVVQEWLRTGRGNLADLKEELWYYNLFINPSADELMNVNRRYGPERTAWLQGFIRNN
                     GA"
     gene            1712..>1991
                     /locus_tag="ACHKIR_005220"
     CDS             1712..>1991
                     /locus_tag="ACHKIR_005220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HFO9992803.1"
                     /translation="MSEWRKNRFEITGKSVCLDVLTQWIEGRETPRYRHAIQQSIQLF
                     LAGCAGLVKPTRTTQYPPYPALVRHGTGLSSPANQAFEQWLGLLVKNAV"
BASE COUNT          479 a          446 c          599 g          467 t
ORIGIN      
        1 gttgctgtgg ctggccgcgg atgaagccgg gtatgtcagc aatgactggc tgacgtaccg
       61 tcaggcgcaa caactgggtg gccacgtccg taaaggggag gtggccacgc tggcggtgat
      121 ttacaaagac tggaaaaaac aggctgtgga tgaacacggt aaccggctga cggacagtga
      181 gggggagcct ctcatggaaa tcgtaccggt actgaaatcg aatcctctgt ttaatgtggg
      241 gcagtgtgac ggtttacctg aacatctgat ggtgaaggcg gaaggcagcg cggatgcact
      301 gccggcagat accttcagtg agcgggtgct gcaggtactg aatggcagcg gcgtcagcgt
      361 ctcgtcactt ccacaggaca gggcattcta ccggcctgat cttgaccgta tcgtgatgcc
      421 actgtcgtcg cagtttgacc aggtggctga ttactggacc accctgctgc atgagctggt
      481 acacagtacc ggtcatgaaa agcgcctgcg gcgggtgggg ataagtacct ttaccggtcg
      541 caacagcccg gaatatgcgt ttgaagagct gattgccgag atgggcagtg cttttctgtg
      601 tgcacatctc gggattgccg gtgacgtaca gcacgaaagt tatattgacg gatggcttaa
      661 ggttctgaag tctgacaaaa aagcgctgtt tcgcgcctgc cggcaggcac gtgaagcatc
      721 agagtattta ctgacgcggc gaacttcgca ggagtgcgca gcataatttt tactaccggt
      781 ggcggttata acctgaccgc catttatccc gtgagggcat tgtccctcac ggggcagtgc
      841 tctctgttat ttcaggatta cagaggaact gagtgatgaa cgataacgca ctggttaatg
      901 ttgacgatgc tgttgtggcc cgtatcaggg atgtgctggc gtatacattt ccgggacaga
      961 aattttctgt gcggaaatgc gatccatctt tcccgaaagt tttcaacaat gtgtgggtga
     1021 gctggctgga tggtccaacg gaggccgaag tacgctctgt cacagagggg tttgagaata
     1081 aagacgggct gctgtctgtg cttgaatccc gttatttcag cgatgaactg gtacaggagt
     1141 gtattgatcg ccttcgtaaa gagtacggac aggggaacgt cccgttgagt gtgaccgtgg
     1201 agcgttaccg gaaaaacgag ctggagaaga ttgaaacagc gcgttttccg ggcaacatat
     1261 ccgtagccat aaacgagatc gggataaata ccagcaaata caggaggtaa ggtatggctg
     1321 gtaaatgcct tgtctggttc tgtaacggga tctctgaacc gtgtctgata gcaggaaata
     1381 tcagtgaatt actggcagat gttacgatca cgtcaaatta ccgtggagat gtcacatctg
     1441 aaatcaggga tgttgtacag gagtggctca ggaccggcag ggggaacctg gcggatttaa
     1501 aggaagagct ctggtactac aaccttttca tcaatccgtc tgcagacgaa ctgatgaatg
     1561 tcaacaggcg gtatggccct gagcgaactg cctggctgca gggttttatc cgcaacaacg
     1621 gggcgtgata ccccgttttc ccgtgagggc attgtacctc acgggacaat gccctctttt
     1681 tacatctgta taaaaaagag gagcgtaaac aatgtctgaa tggcgcaaaa accgttttga
     1741 aattaccggt aaatcagtct gtcttgatgt acttacacag tggattgaag ggcgtgagac
     1801 cccgcgctac cgtcatgcca tacagcagag tatacagctg tttctggccg gttgtgcagg
     1861 gctggtaaaa cccacacgga cgacgcagta tccgccatat ccggcgctgg ttcgtcacgg
     1921 aacggggttg tcatctccgg ctaaccaggc gtttgagcag tggctggggt tgctggtaaa
     1981 aaatgccgtt c
//
LOCUS       DAZGQL010000257         1978 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.259, whole genome shotgun sequence.
ACCESSION   DAZGQL010000257 DAZGQL010000000
VERSION     DAZGQL010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1978
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.259"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..800)
                     /gene="subA"
                     /locus_tag="ACHKIR_005221"
     CDS             complement(<1..800)
                     /gene="subA"
                     /locus_tag="ACHKIR_005221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="subtilase AB5 cytotoxin subunit A"
                     /protein_id="HFO9992804.1"
                     /translation="MLKILWPHILFLLFISVSVRAEKPWYFDAIGLTETTMSLTDKNT
                     PVVVSVVDSGVAFVGGLSDSEFAKFSFTQDGSPFPVKESEALYIHGTAMASLIASRHG
                     VYGVYPHALISSRRVIPDGVQDSWIRATESIMSNVFLAPGEEKIINISGGQKGISSAS
                     VWTELLSRMGRNNERLIVAAVGNDGADIRKLSAQQRIWPAAYHPVSSMNKKRDPVIRV
                     AALAQYRKGETPVLHGGGITGSRFGNGWVDIAAPGQNITFLRPDGKTGT"
BASE COUNT          569 a          442 c          420 g          547 t
ORIGIN      
        1 gtccccgttt ttccatctgg tctgaggaat gtaatattct gtcccggtgc agcaatatca
       61 acccagccgt ttccgaaccg acttccggtg atccctccac catgcaatac aggtgtttct
      121 cctttccggt actgtgccag agctgccact cttatcacag gatcccgctt tttattcata
      181 ctgctgaccg gatggtaagc cgcaggccat attctctgct gagcactcag ttttcgtata
      241 tctgcgccat cattacccac tgccgcaaca attaatcgct cattattccg gcccatacgg
      301 gaaagcagtt cggtccagac cgatgcggaa gatatcccct tctggcctcc cgatatatta
      361 atgattttct cttctcctgg tgcaagaaaa acatttgaca taatgctttc agttgctcta
      421 atccatgagt cctgtacacc gtcaggaata acccttctgc tggatatcag tgcatgagga
      481 taaacaccat aaaccccatg acgtgaggca atgagtgaag ccatggcagt accatgaata
      541 tataaagctt cagactcttt taccgggaat ggcgaaccat cctgagtaaa actgaatttg
      601 gcaaattcgc tgtcgctgag gcctcctaca aatgctactc cggaatcaac aacagatacg
      661 acaacagggg tattcttgtc tgtgagcgac atggttgttt ctgtcaggcc aattgcatca
      721 aaataccagg gtttttctgc cctgacagac acagatataa aaagcaggaa cagaatatgc
      781 ggccataaaa tcttaagcac aatatcctcc agtcagtaca aataaacagt attgcaggtc
      841 aatatcttac aggaacatca catctgaaat atcctgtttt cgacaaaaga taaacaatca
      901 caaccaataa agcgagtgaa gcccgtctca aaaacattaa tttttatatt tatgtatcca
      961 atgaattaac gctcaccttc acggtaatat gcatctggat ttctgcaaca ggtcagcgat
     1021 aagatgctct gcctcctcct tcagagcaga agtaaataca gccatgatgg ataaaactta
     1081 accagaaaaa ataagaggag gcaagtaagt tgcctggcat gtttatgaca ataaaaagtt
     1141 ccgtcaggca tcatggcgaa caggatatca cagcgccgtt ttaccggggc aatcagtgaa
     1201 tggtggtgtg gttcattggt aagccccgga tatcaggtca ctgagtatat tcatgaatga
     1261 ccgttaaacc ggggatgcga gctgctcccc gggaaagtcg aactgactgg tcaatagata
     1321 tcaatacggg gcagtcagtc cacttacagg tatctgacaa ggacaccttc tgagcgtgca
     1381 atgtggacgg gtaatacaac gatggtagca ggctggtcag caatcagcag tatatgccca
     1441 tatttcctga ggccggatat ctgtttcctg agcttgtttt cgacgtttgc taatattttg
     1501 tcgaactaat tatcagaacg attaatgata acagtgtggc agggatcttt accgatatca
     1561 acgtaaataa tgaccttaac aggtgcgtaa ttgctggatt ttgccatgct gttaccctgt
     1621 tgatacgggt taattaaata actttgggga agcaggtacc ggcgttcact accgctctcc
     1681 ctgataatgt atcctgcatg cctgataact tttgccgggc taatgtaaaa taaccctgaa
     1741 atatattaag ttgattaaca gactggtgat ggaaaaaaca ggtattattt attatataaa
     1801 aatccggatt tgatgcggct tgcgatatat tatttttggc ctgtgatggg agcctgaggt
     1861 tgaattatat ataatgcaac tggaggttgc gggataaagt atatcaatta tgtgacatga
     1921 agcagaaagt tcctccccct cttatccaca gacctttccc tttgttgtga tgattttc
//
LOCUS       DAZGQL010000258         1933 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.260, whole genome shotgun sequence.
ACCESSION   DAZGQL010000258 DAZGQL010000000
VERSION     DAZGQL010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1933
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.260"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..842)
                     /locus_tag="ACHKIR_005222"
     CDS             complement(<1..842)
                     /locus_tag="ACHKIR_005222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014728563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HFO9992805.1"
                     /translation="MEMPDLPETPTSDIPTGANNQPWDCTTTGVEMVEKQSRLPTTEN
                     RQYLHIKVEYAMRFPQLSAQIRRKGKTYQMSLKQTVMSGLIRVDEIARNYCWYYKAEV
                     AFDMRTTPPRPFLSSTLLEKDGAGRRHTTNPFPVPGRGIYRRPDVIIVKNKEDRWPGL
                     AGPDTEGNMHSDNLARLVEVKFPGDRLYEDQYEEYMQIVGRNRTRMTILEIHDCRPEE
                     HQWVDQVVNVTMKAWQISGAKYWPLALYPPVFMPRPPSTPVAARIEPWTYAGKVVSDL
                     SEGAV"
     gene            complement(842..>1933)
                     /locus_tag="ACHKIR_005223"
     CDS             complement(842..>1933)
                     /locus_tag="ACHKIR_005223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2345 domain-containing protein"
                     /protein_id="HFO9992806.1"
                     /translation="FANSKLRMEDRRGEEHIKLATEYGKTQLNSGHLVDSQGQLRGKG
                     TELRTDEWGTLRAGKGLFVAADAQAKAQGEALDMDAALKEIDRLNQQLQQLENAAERA
                     QALKADIDSQIQMFAQRLKPLNEAVLFSAPEGIALTSGEDMQMSAARNVAVNAGGDIS
                     TGTMGNLTALAGERLGLFARTGQLSVKSGEGPVEVQAQNGSMRLFAEKKLTISSESDI
                     SFTGKKRITLIGGGSYLKLEAGKVEYGTMATCMRRVKRTMTAAAQSMPMAFPRMSSSH
                     IYSAIYRLLDKHGNILVNTPCRLTTPSGQTVTGYSDNEGRSVPVYTRQQEDVELHFFV
                     KKPQPEETMWFVGETDTQQLETELRESQS"
BASE COUNT          369 a          583 c          487 g          494 t
ORIGIN      
        1 accgccccct cactgaggtc agatactact ttaccagcgt aagtccaggg ctctattctg
       61 gcagcaacgg gggttgatgg cggacggggc ataaagactg gcgggtaaag agccagcggc
      121 cagtatttcg cgccgctgat ctgccaggct ttcatcgtca cgttgaccac ctggtcaacc
      181 cactggtgct cttccgggcg acagtcgtga atctccagaa tggtcatgcg ggttcggttc
      241 ctgcccacaa tctgcatata ctcttcatat tggtcttcat acagcctatc tcccggaaac
      301 ttcacctcca ccaggcgggc caggttatcg ctgtgcatat tgccttcggt atccggcccc
      361 gccagtccgg gccagcggtc ttccttattt ttaacaataa tcacgtccgg gcggcggtaa
      421 atgcccctgc cgggcacagg gaaaggattg gtcgtgtggc gacggccagc accgtctttt
      481 tccagcagcg tactgctcag aaaagggcgt ggcggcgtgg tgcgcatatc aaacgccacc
      541 tccgccttat aataccagca gtaattgcgg gcgatttcat ccacgcgaat aagaccactc
      601 ataacggtct gcttaaggct catctggtaa gttttacctt tccggcggat ttgtgccgac
      661 agttgcggaa aacgcatggc atattccacc tttatatgca ggtactgacg gttttccgtg
      721 gttggcagac gcgactgctt ttcgaccatt tcgacacccg tcgtggtgca gtcccacggc
      781 tggttgttcg ccccagtggg aatatcagat gtcggtgtct cgggtaaatc gggcatttcc
      841 attatgactg gctctccctg agttcggttt ccagttgctg cgtatcggtt tcccccacaa
      901 accacatcgt ttcttcgggt tgtggttttt taacaaaaaa atgcagttcc acatcctcct
      961 gctggcgggt gtaaaccggc acggagcggc cttcgttatc gctatacccc gttactgtct
     1021 gacctgatgg agtggtgagg cgacaggggg tattgaccag aatattgccg tgtttgtcca
     1081 gcaacctata gatagccgag tagatatgcg aagaactcat acgggggaat gccatcggca
     1141 tactctgcgc agccgccgtc atcgtccgct tcacccgacg catacaggtt gccatcgtgc
     1201 catattcaac cttcccggct tccagtttca ggtaactgcc accgccaatc agcgtaatgc
     1261 gtttcttgcc ggtaaacgag atatcacttt ctgacgaaat ggtcagcttc ttctcagcaa
     1321 acaaccgcat gctgccgttt tgcgcctgta cctctactgg cccctccccg gatttgacgc
     1381 tcagttgtcc ggtcctggca aaaagcccca gcctttctcc ggccagcgcg gtcaggttgc
     1441 ccattgttcc ggtactgata tcacctccgg cgttgacagc cacatttcgt gctgcactca
     1501 tctgcatatc ttcaccgctg gtcagggcaa tgccttccgg agccgagaaa agcacggctt
     1561 cattgagcgg tttcagccgc tgcgcaaaca tctgtatctg actgtcaata tcagccttca
     1621 gcgcctgtgc ccgttctgcg gcattttcca gttgctgcag ttgctgatta agcctgtcga
     1681 tttccttcag cgcggcatcc atgtccagtg cctcgccctg tgctttcgcc tgcgcatccg
     1741 cagcaacaaa aagtcccttc cccgctctca gggttcccca ctcatcggta cgcagctctg
     1801 tgcctttacc ccgcagttgc ccctggctgt ccaccagatg tccgctgtta agctgggtct
     1861 taccgtactc cgtggcgagt ttgatatgct cctcgccgcg ccggtcctcc atccgcagtt
     1921 ttgagttagc gaa
//
LOCUS       DAZGQL010000259         1910 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.261, whole genome shotgun sequence.
ACCESSION   DAZGQL010000259 DAZGQL010000000
VERSION     DAZGQL010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1910
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.261"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..1772)
                     /gene="tssD"
                     /locus_tag="ACHKIR_005224"
     CDS             complement(<1..1772)
                     /gene="tssD"
                     /locus_tag="ACHKIR_005224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein TssD"
                     /protein_id="HFO9992807.1"
                     /translation="MSDIVYLRIIGEKQGYISSGCGTYASVGNCWQVGHEDEIFAFAL
                     TNAITSTGKGVNLQGLQFCKLIDKSSPLLSNAINQNERLFIEIDLYRINKSGRWERYY
                     YIQLRNASLTAIHVNISDNNLPTECITVDYDYILCKHLIVNTEFDWLAFPAGYNSLFI
                     PPKSPPASNLKPEPLPVVNLPLSSPAVNPVYAKSCLKEKGCTDAGTAEEPAENFGQVA
                     IFAQPVVDDCCGYHHPEANDVGQPAEAQTMLLFPGSVLAAQIWGKWSLSGILSATRGS
                     YIGALASALYIPSAGEGSARVPGRDEFWYEEELRQKALAGSTATTRVRFFWGTDIHGK
                     PQVYGVHTGEGTPYENVRVANMQWNEQTQRYEFTPAHDVDGPLITWTPENPEHGNVPG
                     HTGNDRPPLDQPTILVTPIPDGTDTYTTPPFPVPDPKEFNDYILVFPAGSGIKPIYVY
                     LKEDLRKLPGVVTGRGVPLSLGTRWLDMSVSNNGNGAPIPAHIADKLRGREFKTFDEF
                     REALWLEVSKDSVLLAQFIKSNQNNVSQGYSPYVPEEGYYYGPNEIVKKFQIHHVVAI
                     EHGGGVYDIDNFRIVTPRLHDEIH"
BASE COUNT          511 a          470 c          415 g          514 t
ORIGIN      
        1 tagtgaattt catcatgtag tcggggcgta acaatcctaa aattatcaat atcatacact
       61 ccaccaccat gttctatagc aactacatga tgtatctgga atttcttaac tatctcattt
      121 ggtccgtaat aatacccctc ctcagggaca tagggggaat atccttgact aacgttattc
      181 tgattacttt ttataaactg cgccaataac actgagtctt tactcacctc cagccacagc
      241 gcctcgcgaa actcatcaaa ggttttaaac tcccgtccgc gcaatttatc agcaatatgc
      301 gccgggattg gtgcgccgtt gccgttattg gataccgaca tatccagcca gcgagttcct
      361 agtgaaagcg ggacgccgcg ccctgttaca acaccaggca gctttcgcag atcctccttc
      421 aggtaaacat agatgggctt aataccggat cccgccggaa aaaccagaat ataatcgttg
      481 aattctttcg gatcaggaac cgggaatggc ggcgtggtat aggtatcggt gccgtccgga
      541 atcggcgtca ccagaatggt gggctgatcc agcggcggcc tgtcgttacc ggtatggccc
      601 ggaacattcc catgttccgg attttccggc gtccaggtaa tcagggggcc atcgacatcg
      661 tgagcggggg taaattcata acgctgcgtc tgctcgttcc actgcatgtt cgccacgcgg
      721 acgttttcat acggcgtacc ttcacccgta tgaacaccat acacctgagg cttgccgtga
      781 atgtcagttc cccagaaaaa acgtacccgg gtggtggcgg tactgcctgc tagtgctttc
      841 tgccgcagtt cttcctcata ccagaactca tcacgtccgg gcacgcgagc actgccctcg
      901 cccgcagagg gaatatacaa agcagatgcc aacgcaccga tgtaagagcc gcgggttgca
      961 ctgagtatgc cactgagcga ccattttccc catatttgag ccgccaacac actacccgga
     1021 aacagtagca tggtttgagc ttctgcgggt tgcccgacat cgttagcctc gggatggtgg
     1081 tatccacagc agtcatcaac cactggctga gcaaaaatcg ctacttgacc gaagttttca
     1141 gcgggttctt ctgccgttcc agcatctgta catcccttct ccttcagaca ggattttgca
     1201 tagaccgggt taaccgctgg tgaagagagt ggaaggttaa caactggtag aggttcaggc
     1261 ttaagattac tggcaggtgg gcttttaggc ggaataaata agctattata gccagcagga
     1321 aaggccaacc agtcaaattc cgtattgact attagatgtt tacataatat gtagtcataa
     1381 tcgacagtta tacattcggt aggaagatta ttgtcagaaa tgtttacatg aatagcagtt
     1441 aatgaagcat ttcttagctg aatataataa taccgttccc agcgcccgct tttatttata
     1501 cgatacaaat cgatttcaat aaataaccgc tcattctgat tgatggcatt agacagtagc
     1561 ggtgagcttt tatcaatgag tttgcaaaat tgtagcccct gtagattaac gcctttacca
     1621 gtactggtaa tggcgttggt gagtgcaaag gcaaaaattt catcttcatg gccaacctgc
     1681 cagcaattac caaccgacgc atacgtccca cagcctgaag aaatatagcc ctgcttctca
     1741 ccgattattc tcaggtaaac aatatcgctc actttatttc attccagttt atccttaact
     1801 tatttgcatt aaatagaaaa cttaattctc gatcaataag aaacgctttt aatgaaagat
     1861 tatttcaatt acttaacgtt ttcacttagc tcgcaatatt atatttaaat
//
LOCUS       DAZGQL010000260         1902 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.262, whole genome shotgun sequence.
ACCESSION   DAZGQL010000260 DAZGQL010000000
VERSION     DAZGQL010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1902
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.262"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..240
                     /locus_tag="ACHKIR_005225"
     CDS             <1..240
                     /locus_tag="ACHKIR_005225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992808.1"
                     /translation="CFTVRLKGDRRGFIDLLVISPDGQRCAVEVDNRSPRQRSLMKIR
                     ALPEGISGFVFLRDGKHPQRYIADGVDVIRATRFK"
     gene            274..>1902
                     /locus_tag="ACHKIR_005226"
     CDS             274..>1902
                     /locus_tag="ACHKIR_005226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFO9992809.1"
                     /translation="MLNIKPNFAQERALNMLRHDWKTYQSFMMYMPTGSGKTGLAAFI
                     TAGLVSRGMRVLFVVPYTILINQTAQRFVQYGLSGDEISFIWRDHPNNDPSRLIQIAS
                     ADTLIRRDFPQNIDLLIIDEAHLRKRRILTEIERLVAEGNVKVIGLSGTPFSSFLGRY
                     YQRLLKPTTIGELIQRGELSNYEFYAPTKPDLEGVKTKVSMEYGRDYDESQLAEIMCG
                     ADLVGDIVDNWLCNGRDLPTVAFCVNKTHASFVTMQFNKAGINAEVMVAETPHDERQL
                     MISRFETGATKIIVSVGVLVAGFDSDVRCIIYARPTKSEIRWLQAIGRGLRTAPGKES
                     CLIFDHSGTVHRLGFPENIEYDELPGKNDGMQVSSSRGNEIREEKLPKECPGCHFMKP
                     AGVYVCPKCGFKPLAGEDVNTDTRRNIRKLSKDEKIYTKSDKQAWWSQIKFYQRQRAS
                     LGRPVSDGWCAHTFREKFGEWPDGLSSFPMEITPEVSNYIRHKFIRFARGRSRAEKMT
                     EKSPEALSLSLLHGVRSGVPEGSESWQIMQAKQQL"
BASE COUNT          472 a          439 c          530 g          461 t
ORIGIN      
        1 tgtttcactg tcaggctgaa aggtgatcgc aggggattta tcgatcttct ggttatttct
       61 cccgatggtc agcgttgcgc agttgaggtg gataaccgat cgccccgtca gcgttcactg
      121 atgaaaattc gagcgcttcc tgaaggcatt tcgggatttg tttttctccg tgatggcaaa
      181 catccacagc gttatatcgc cgatggtgtg gatgtcatcc gggcgacccg gtttaagtaa
      241 tcactgtatc agccctgtca tcgggagaat accatgctga atatcaagcc gaattttgct
      301 caggaacgtg cgctgaatat gttgcgtcat gactggaaaa catatcagtc cttcatgatg
      361 tacatgccta cagggagtgg taaaaccggc ctggcggcgt tcatcactgc tggtctggta
      421 agccgtggca tgcgcgttct gtttgtcgtt ccttatacca ttttgatcaa ccagacagca
      481 cagcgctttg ttcagtacgg gctttccggg gatgaaatca gctttatctg gcgtgatcat
      541 cccaataacg acccgtcacg actgattcag attgccagtg cggatacgct gattcgccgt
      601 gattttcctc agaatatcga cctgctgatt attgatgaag cgcatctccg taagcgccgc
      661 atcctcacgg aaatcgaacg actggttgca gagggaaatg tgaaggtgat tgggttgtcc
      721 ggtacaccct tttcatcgtt cctggggcgt tattaccagc gtcttctcaa gccgaccact
      781 ataggggaac tgatccagcg tggcgaactg agcaattacg aattttatgc gcccacgaaa
      841 ccggatctgg aaggtgtaaa aacgaaagtc tcaatggagt acggcaggga ttacgacgaa
      901 tcacaactgg cagagattat gtgcggcgcg gatctggttg gcgatattgt cgataactgg
      961 ctgtgtaacg gaagggattt accgacagtg gcgttctgcg tcaataaaac gcatgccagc
     1021 ttcgtgacca tgcagttcaa caaagccgga attaacgcag aggtgatggt tgctgaaacg
     1081 ccacatgatg agcgtcagtt aatgatcagt cgttttgaaa cgggagcgac gaaaatcatt
     1141 gtcagcgttg gcgttctggt ggcgggattt gacagtgatg tccggtgcat catttacgca
     1201 cgcccgacaa aatcagagat tcgctggctt caggcgattg ggagaggatt gcgtacagcg
     1261 ccaggcaagg agtcatgcct gattttcgac cattccggta cggttcatcg cctgggattt
     1321 cctgaaaata ttgagtatga cgaactgccc ggcaaaaacg acgggatgca ggtgtcatca
     1381 tcacgtggta acgaaattcg ggaagaaaaa ctgccgaaag aatgtccggg gtgtcatttc
     1441 atgaaaccgg caggtgttta tgtctgtccg aaatgcggat ttaaacctct ggctggtgag
     1501 gatgtgaaca cagatacccg ccgcaatatc aggaaactca gcaaggacga aaaaatttac
     1561 accaaaagcg acaaacaggc ctggtggagt cagatcaaat tctatcagcg tcagcgtgcc
     1621 tctctggggc gaccggtcag cgatggatgg tgcgctcaca ctttccggga gaagttcggc
     1681 gaatggcctg acgggctgag cagttttccg atggaaatca ccccggaggt cagtaattac
     1741 atcagacaca aattcatccg gtttgccaga gggcgtagcc gggcagagaa gatgacggaa
     1801 aaatcccccg aagcgctttc tctgtccctg cttcatggtg tccggtctgg ggtgccggaa
     1861 ggcagtgagt cgtggcagat catgcaggca aagcaacaac tt
//
LOCUS       DAZGQL010000261         1898 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.263, whole genome shotgun sequence.
ACCESSION   DAZGQL010000261 DAZGQL010000000
VERSION     DAZGQL010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1898
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.263"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..1060
                     /locus_tag="ACHKIR_005227"
     CDS             <1..1060
                     /locus_tag="ACHKIR_005227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HFO9992810.1"
                     /translation="MGPKGEKGDPGGPAGPQGPKGERGEAGPQGPMGARGERGETGPR
                     GEPGPAGPRGERGETGPQGPRGEPGPAGSAANVADATTAQKGIVQLSSATDSDDETKA
                     ATPKAVKAAMDKADGCLEKAKNGDDIPDKVKFLNTVGAARVYGRDIHTGAGEWTTSEF
                     VAWLKEKGAFDQPYWMMKASLLAEFNKVITDVGPGKLNLGGCAIEVMGTYNAAIVRVT
                     IGEYGGDGFLNGTVCTCTVYGDTQRFHWRVDYSTKNKPTTASLTVNGWERDEVTGRLR
                     QWGSIEVSEDDGKLMTQHSIRFPVAFPVAALNAQVSAVGDPHAIKMYTLSNPSLVSVT
                     LITVRGCYGKFSWEAIGY"
     gene            1070..1294
                     /locus_tag="ACHKIR_005228"
     CDS             1070..1294
                     /locus_tag="ACHKIR_005228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9992811.1"
                     /translation="MEQYVFSPSENMFYPLSLRPVYEAAGRWPEDGIVVDYVVYKVFA
                     ADAAPAGMKRGVGAEKMPVWVPVSEEGTET"
     gene            1294..>1898
                     /locus_tag="ACHKIR_005229"
     CDS             1294..>1898
                     /locus_tag="ACHKIR_005229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFO9992812.1"
                     /translation="MHIRNFSYYTPAEPDVAGAMYLKSEDGQDWYECQALFSPETLKV
                     VYDSRGVITGYGKDTALLWPVNQSVAEVPDTPESRKIDLSGRWGFDGEKITDLLTAEK
                     ARGMKGDEINAWRNAMEAANYTFEHNGRKWDYGKSTQTRLEPSVAAAKSGKLPEAFFW
                     TDAENNDVEVTAEELIALSEAAEQAMFTKGMEIHVRQRTMK"
BASE COUNT          481 a          402 c          652 g          363 t
ORIGIN      
        1 tatggggcca aaaggcgaga aaggtgatcc gggaggtcct gcaggcccgc aaggtcctaa
       61 aggtgaacga ggagaagccg gaccacaggg accgatggga gcacgaggtg agcgtgggga
      121 gactggcccc cgaggtgaac ctggtcctgc aggtccgaga ggcgaacgag gagagaccgg
      181 acctcaggga cctcgtggag agccaggtcc ggcaggcagc gctgcaaatg tggctgatgc
      241 gacgacggca cagaagggaa ttgtgcagtt aagcagcgca acggacagtg atgatgaaac
      301 gaaggcagcc accccgaaag cggtgaaagc ggcaatggac aaagcggacg gatgcctgga
      361 gaaagcgaaa aacggtgacg atatcccgga taaggtgaag tttctgaaca ccgtgggggc
      421 agccagagta tacgggcggg acattcatac gggggccggt gaatggacca cgagcgagtt
      481 tgtggcctgg ctgaaagaaa agggggcatt tgaccagcct tactggatga tgaaggcatc
      541 actgcttgcg gaatttaata aggtcatcac ggatgtcgga ccgggaaaac tcaatctggg
      601 aggctgcgcc attgaggtga tggggacgta taacgcagcc atagtccggg tcaccatcgg
      661 cgaatacggt ggcgatggtt ttctgaacgg cacggtctgt acctgtacag tttacggaga
      721 cacacaacgt tttcactggc gggtggatta cagcacaaaa aacaagccca caacagcaag
      781 cctgacagta aatggctggg agcgtgatga agtaaccggt cgtttgcggc aatggggcag
      841 tattgaggta agtgaggatg atggtaagct tatgacacag cactctatcc gttttcctgt
      901 cgcttttccg gttgctgctc tgaatgctca ggtgtctgca gtgggagacc cccacgccat
      961 taagatgtat accctgtcga atccgtcatt agtgtctgta actctgataa cagtcagggg
     1021 atgctacggc aagttttcat gggaagcgat cggatactga gggcattgta tggaacagta
     1081 tgttttttca ccgtcagaga atatgttcta tcccctgtcc ctgcggccgg tttatgaggc
     1141 agcagggcgc tggccggaag acggtattgt ggtggattat gtggtgtata aagtctttgc
     1201 agcggatgct gcaccggcag gtatgaagcg gggcgtcggt gcggaaaaaa tgccggtctg
     1261 ggtgccggta tcggaggagg gcacggaaac atgatgcata tcagaaattt ttcgtattac
     1321 accccggcgg agccggatgt tgcaggcgcg atgtacctga agtctgagga tggtcaggac
     1381 tggtatgagt gtcaggcgtt gttttcgccg gagacgctga aggtggtgta tgacagccgg
     1441 ggtgtgatta cgggatacgg taaggacact gcgttactgt ggccggtgaa ccagagtgtg
     1501 gcagaagtgc cggatacccc ggagagccgg aaaattgacc tgagcgggcg ctggggtttt
     1561 gacggggaga aaatcacgga cctgctgacc gcggagaaag cgcgagggat gaagggcgat
     1621 gaaataaacg cgtggcgtaa tgcgatggaa gcggcgaact acacgtttga gcacaatggg
     1681 cgtaaatggg actacgggaa gtcaacgcag acgcgtcttg agccgtcggt ggcggcagcg
     1741 aaatcgggga aactgccgga ggcgtttttc tggacggatg cggaaaacaa tgatgtggag
     1801 gtgacagcag aagagcttat agcgctgagt gaagcggcag agcaggcgat gttcaccaaa
     1861 gggatggaaa tccacgttcg ccagcgcacc atgaagaa
//
LOCUS       DAZGQL010000262         1858 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.264, whole genome shotgun sequence.
ACCESSION   DAZGQL010000262 DAZGQL010000000
VERSION     DAZGQL010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1858
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.264"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..983
                     /locus_tag="ACHKIR_005230"
     CDS             <1..983
                     /locus_tag="ACHKIR_005230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFO9992813.1"
                     /translation="RYDFNEKIDFWYVIALAGQSNGMAYGEGIPLPDTLDKPESRVKQ
                     LARRKTITPGGKECKFNEIIPADHCLHDVQDMSGYHHPAADLHKGEYGCVGQGLHIAK
                     KLLPYIPEQAGILLVPCCRGGAAFTVGAEGMYVPDTGATADAMRWGTGTALYEDLVAR
                     VKVALEYNRKNKLLSVCWMQGEFDLMSPDYEKHPDLFYQMVTSFRSELSEYSSQCVGN
                     SSERVPWLCGDTTWYWKESYQKEYDFIYGHYRQRTDDEIHFLSFQDSNRHELTNEPEE
                     DADDLSVGYLGSSWRTELSWTTSQRSTHFNSMARRGVIAECYAQKIRNYL"
     gene            complement(1298..1720)
                     /gene="subB"
                     /locus_tag="ACHKIR_005231"
     CDS             complement(1298..1720)
                     /gene="subB"
                     /locus_tag="ACHKIR_005231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_062863141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="subtilase AB5 cytotoxin subunit B"
                     /protein_id="HFO9992814.1"
                     /translation="MTIKRFFVCAGVMGCLSLNPAMAEWTGDARDGMFSGVVIDQFHT
                     GQIDNNPYFCIEGKQPGGSSIRACSMKNSSVWGPSFSTLYNQALYFYTTGQLVRIYYE
                     PGVWTYPPFVKALTSNALVGLSTCATSTECFGPDRKKN"
     gene            complement(1737..>1858)
                     /locus_tag="ACHKIR_005232"
     CDS             complement(1737..>1858)
                     /locus_tag="ACHKIR_005232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HFO9992815.1"
                     /translation="PSLADKVTEGRVLNAEKAISMFCKKNYIPVRQGRMSEEL"
BASE COUNT          544 a          386 c          424 g          504 t
ORIGIN      
        1 atcgttatga ttttaatgaa aaaatagatt tctggtatgt gatagccctc gcaggacagt
       61 ctaatggtat ggcctacggt gaaggtattc ccctgccaga tacactcgac aaacccgagt
      121 cccgtgtaaa acaactggct cgtcgaaaaa caatcacacc aggaggaaaa gagtgtaaat
      181 tcaatgagat tattccggca gatcactgtc tgcatgatgt tcaggatatg agtggatatc
      241 accatccggc tgctgatttg cataaagggg aatatggttg tgtcggacag ggattgcata
      301 tagctaaaaa gttactgcca tacatacctg agcaagcagg gatattactg gttccttgtt
      361 gcagaggagg tgccgccttt actgttggag ctgaaggtat gtatgtacct gatacagggg
      421 caacagctga tgcaatgcgg tggggcaccg gaacagcact ctatgaggac ttagtagccc
      481 gggttaaggt agctctggaa tataacagaa agaataaact gctatctgtc tgctggatgc
      541 agggggagtt tgatcttatg tcaccagatt atgaaaagca tcctgacttg ttttatcaga
      601 tggtaacttc tttcaggtct gagttatctg aatactcatc tcagtgtgtt ggtaattcgt
      661 cagaaagagt tccatggttg tgtggtgata caacctggta ctggaaggaa tcttatcaaa
      721 aagaatatga ttttatatat ggacattaca gacaacggac tgatgatgaa atacattttc
      781 tcagctttca ggatagtaac cgacatgaat taactaatga gccagaagaa gatgccgatg
      841 atttatctgt cgggtatctg gggtcatcat ggaggacaga attaagctgg acaacgtcac
      901 agagaagtac acactttaat tcaatggcca gaaggggagt tattgctgaa tgttatgcgc
      961 aaaaaataag aaattatttg tagacaagtt caggatatat ctacagctct gacatgattc
     1021 tctgagagac atgacggagt ggtatggagt tgtcggtatc aacaccaaga tagctggtcg
     1081 ttaccgtttg atatcactgg ttctgatact atgtggacgg tagtgtgtca cgaaggcacg
     1141 caccagggac gtgagtccct gtaatatcat cgacagtccc attatggcat gtctgcacct
     1201 acaacgtcat ttcgaggtcg gctcagtgtt cactcgagtc ttatatcact gacgccgaca
     1261 ctccatccag agtatctctg gacggaaaca tcacctatta gttctttttt ctgtcagggc
     1321 caaaacattc agtcgatgtg gcgcaggtcg ataacccaac aagggcattg gacgttaatg
     1381 ccttcacaaa gggcggatac gtccataccc cgggttcata ataaatcctg accaactgac
     1441 ctgttgtgta aaaatatagt gcctgattat ataatgtgga aaacgaaggc ccccagacag
     1501 atgaattctt catcgagcag gctcttatgg aagaaccacc aggttgtttc ccttcaatac
     1561 aaaaataagg gttattatct atttgccctg tatgaaactg atcaataaca acgcctgaaa
     1621 acatgccatc ccgggcatcg ccagtccact cagccatggc aggattaagg gataaacatc
     1681 ccataacacc tgcacataca aaaaacctct taatcgtcat attcacctcc ggaattttac
     1741 agttcttcac tcatccttcc ctgacggaca ggaatataat ttttcttgca gaacatgcta
     1801 attgcttttt ctgcattaag aactctccct tccgtcacct tatctgcaag tgaggggt
//
LOCUS       DAZGQL010000263         1844 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.265, whole genome shotgun sequence.
ACCESSION   DAZGQL010000263 DAZGQL010000000
VERSION     DAZGQL010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1844
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.265"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..385
                     /locus_tag="ACHKIR_005233"
     CDS             <1..385
                     /locus_tag="ACHKIR_005233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFO9992816.1"
                     /translation="LLTAEKARGMKGDEINAWRNAMEAANYTFEHNGRKWDYGKSTQT
                     RLEPSVAAAKSGKLPEAFFWTDAENNDVEVTAEELIALSEAAEQAMFTKGMEIHVRQR
                     TMKKELEKLTSADEILAYRVGWAQA"
     gene            461..643
                     /locus_tag="ACHKIR_005234"
     CDS             461..643
                     /locus_tag="ACHKIR_005234"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992817.1"
                     /translation="MTMLVYGVKTATEMLILYVMLILFPEHPVDVNNFQIFGSESLDI
                     SIYATVDVFCGAWQCD"
     gene            complement(1588..>1844)
                     /locus_tag="ACHKIR_005235"
     CDS             complement(1588..>1844)
                     /locus_tag="ACHKIR_005235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5339 domain-containing protein"
                     /protein_id="HFO9992818.1"
                     /translation="ITVISCTTASAAMTETCQKYFDETDKFLSQMKTTGVAEGQINLM
                     KQQYEQSKKQFSSLPEASQDAACKQASDALKQSMSAAGIK"
BASE COUNT          516 a          348 c          461 g          519 t
ORIGIN      
        1 cctgctgacc gcggagaaag cgcgagggat gaagggcgat gaaattaacg cctggcgtaa
       61 tgcgatggaa gcggcgaact acacgtttga gcacaatggg cgtaaatggg actacgggaa
      121 gtcaacgcag acgcgtcttg agccatcggt ggcggcagcg aaatcgggga aactgccgga
      181 ggcgtttttc tggacggatg cggaaaataa tgatgtggag gtgacagcag aagagcttat
      241 agcgctgagt gaagcggcag agcaggcgat gttcaccaaa gggatggaaa tccacgttcg
      301 ccagcgcacc atgaagaagg agctggaaaa gctgaccagt gcggatgaga tactggcata
      361 cagagttggc tgggcacagg cgtaataaaa aaggggggct gataaacagc ccctgaccaa
      421 agaaaagtat cagcggaaac atttagaagg atctaaataa atgaccatgc ttgtatacgg
      481 cgtaaagact gccacggaaa tgttaattct gtacgtgatg ctgatcttgt ttccggagca
      541 tcctgttgat gtgaataatt ttcagatttt cggaagtgag agtttggata tttccatata
      601 tgccacagtt gatgtgtttt gtggcgcctg gcagtgtgat taagggaaca gggataacga
      661 aaaaagcggg ggtatcacca ccccccggta caaggaagta ttaactataa tggaaacatt
      721 aatgaagaca ttcagtgcta tgttatatgg caaataaaga ctgccacagg gaagtttgca
      781 gaaacagtga tgccgatcgt gtttctgtgc tcttctctga taagagtaat ttttcagatt
      841 tccggaagtc cgtattttgc acgggcccgg ttgtatgctt cagactgcgg tggatatcaa
      901 ggtcacggag ttggtcggtt gcaaggaata gattgagaat tgagcgtgtt tctggcacat
      961 gcctgactgt aatcatccct acaaactaca ttgatgccag aaacgcactg gtaacagggc
     1021 atcgtgtatt tcagtatgtc cagtttttgt tgcggtgatt gcataaaaaa tcccccttga
     1081 ggggtgaaat atcaccggaa ttttcggcaa gtatttttat cgggttgagt gtatttctgg
     1141 ggctctgctg aggcaattca ctgaatgtgg cttttgtgct cgctggaaat gcactcatac
     1201 actttttaac gcgatttttt tcaggcaata gtttttaatt tgttagcgtt ctcgctgtca
     1261 ggcacgtttt gagcacgttt ccgactctca gctacagcaa tccacgacta gggacagcac
     1321 ttacgtttta cttagcgcac cgtaaaactc cgctcttcag ggtaagggct tacatgaaga
     1381 aaaaaaataa acttgtgaat atgaaagtaa atactataaa aagatggttc ctcttttttc
     1441 tcacgtgctg aataatataa acaatagtgc atgatacaaa agacactaat aaccacaaag
     1501 ttatgatacg gagcacgatt tatgtcttcc tgattcagtg tgtcaagaaa cagttatcaa
     1561 tatacaataa ctgtttcttt tattatatta tttaattccg gcagcgctca ttgattgctt
     1621 aagcgcgtca cttgcttgtt tacaggcagc atcctggctt gcctcgggca gagatgagaa
     1681 ttgtttcttt gattgttcat attgttgttt cattaaatta atctgtcctt ctgctactcc
     1741 ggttgttttc atttgcgata agaacttatc tgtttcatcg aaatacttct gacatgtttc
     1801 tgtcatagcc gcactggcag ttgtgcaaga tattacagtg atta
//
LOCUS       DAZGQL010000264         1844 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.266, whole genome shotgun sequence.
ACCESSION   DAZGQL010000264 DAZGQL010000000
VERSION     DAZGQL010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1844
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.266"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>1844
                     /locus_tag="ACHKIR_005236"
     CDS             <1..>1844
                     /locus_tag="ACHKIR_005236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000431134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO9992819.1"
                     /translation="LLRRLNHYCARALEGAASLCQTRAHAEITPEHWLLKLLEQGEGD
                     LTVLGRRYDWDMDAIWQSLLGWLDNQPRSVRSRPQLAPSLNALLKQAWMVASLQGEEH
                     IRSVHLLGALTENPHLVRCDGVWPLLTLSQSQLQRLSPLLDAQSDECPERLQDAEPVL
                     PQGDNVTFIGRPVGADTAGIPSGDLPPVLQSALDKFTRYITASAREGKIDPVSGRDTE
                     IRQMVDILSRRRKNNPILVGDPGVGKTALVEGLALRIAEGNVPESLRPVTLRTLDLGL
                     LQAGAGVKGEFEQRLKNVIDAVQQSPAPVLLFIDEAHTLIGAGNQAGGADAANLLKPA
                     LARGELRTIAATTWSEYRQYLERDAALERRFQMVRVDEPDDETACLMLRSLKSRYAEH
                     HNVHITDEAVRAAVTLSRRYLTERQLPDKAVDLLDTAAARVRMSLDTVPEQLTRIRSQ
                     LASLGMEKQALLEDIAVGHQNHGERLSAIEQEENELMTARDDLEQQYARECELTGELL
                     ESRSDISRQSETLHLQQALHDIQQNQPLLSVDVDVRTVAGVVADRTGVPLSSLMKDEQ
                     TELLHLEKDIGRRVVGQDVALESIAQRLRAAKTGLTSGNGPQGVFLLVG"
BASE COUNT          379 a          514 c          583 g          368 t
ORIGIN      
        1 ctgttacgtc gtcttaatca ttactgtgcc cgtgcactgg aaggcgcagc ctccctttgc
       61 cagacccggg cccatgcaga aatcaccccg gagcactggt tactgaaact gctggagcag
      121 ggggaaggag accttaccgt gctgggccgg cgttatgact gggatatgga cgcgatatgg
      181 cagtcgctgc tcggctggct ggataaccag ccccgtagcg tacgcagccg cccgcagctt
      241 gcgccgtccc tgaatgccct gctgaaacag gcctggatgg tggcctcatt acagggagaa
      301 gaacatatcc gcagcgtgca tctgctgggt gccctgacgg aaaatccgca cctggttcgc
      361 tgtgacgggg tgtggcccct gctgacactg agtcagagcc agttgcagcg tctgtccccg
      421 ctgctggatg cgcagtctga tgagtgtccg gagagattac aggatgcaga gcctgtgctg
      481 cctcagggag ataatgtgac ctttatcggg cgtcccgtcg gtgcggatac ggcgggtata
      541 ccgtcaggtg acctgccgcc tgtgttacag agtgcgctgg ataaattcac ccggtacatc
      601 acggccagcg cgagagaggg gaaaattgac ccggtatcgg gacgcgatac ggaaatccgt
      661 cagatggtgg atattctctc ccgccgccgc aagaacaacc cgattctggt gggggaccct
      721 ggtgtgggga aaacggctct ggtggaaggg ctcgccctgc gtattgcgga aggtaacgta
      781 ccggaatcac tcagacccgt caccctgcgc acccttgacc tcggcctgct gcaggccggt
      841 gccggcgtga aaggtgaatt tgaacagcgc ctgaaaaacg tgattgatgc cgtgcagcag
      901 tcaccggctc ctgtactgct gtttatagat gaagcccata cccttatcgg tgccggtaat
      961 caggccggtg gcgcggatgc ggccaacctg ctgaagcctg cgctggcgcg gggtgaactg
     1021 cgcaccattg ccgccaccac ctggtctgag tacagacaat acctggaacg tgacgcagcc
     1081 ctggagcggc gttttcagat ggtcagagtg gacgagccgg atgatgagac ggcctgtctg
     1141 atgctgcgct ccctgaaatc ccgttatgcg gaacatcata acgtgcatat caccgatgag
     1201 gcggtacgtg ctgccgtcac gctgtcgcgc cgttatctga cagaacgcca gttaccggac
     1261 aaggccgttg acctgctcga tactgctgct gcccgtgtgc gtatgagcct cgacacggtg
     1321 ccggaacagc tgacccgtat ccgttcacag ctcgcttccc tcggtatgga aaaacaggca
     1381 ctgctggaag atattgccgt tggtcatcag aatcacggcg aacgtctgtc tgccattgag
     1441 caggaagaga acgagctgat gacagcacgt gatgacctgg agcagcagta cgcccgggag
     1501 tgcgaactca ccggtgagtt acttgaaagt cgcagcgata tttcccgtca gagtgagaca
     1561 ctgcatctgc agcaggcact gcacgacatt cagcagaatc agcccctgct cagtgtggat
     1621 gttgacgtac gcaccgttgc cggtgtggtc gcggaccgga ccggggtgcc gttatcttcc
     1681 ctgatgaagg acgaacagac ggaactgctg catctggaaa aggatatcgg cagacgggtg
     1741 gtcggacagg acgtggcact ggagtccatt gcgcagcgtc tgcgtgcggc gaaaacgggt
     1801 ctgacatccg gtaacggccc ccagggggtg ttcctgctgg ttgg
//
LOCUS       DAZGQL010000265         1843 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.267, whole genome shotgun sequence.
ACCESSION   DAZGQL010000265 DAZGQL010000000
VERSION     DAZGQL010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.267"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..332)
                     /locus_tag="ACHKIR_005237"
     CDS             complement(<1..332)
                     /locus_tag="ACHKIR_005237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002445141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFO9992820.1"
                     /translation="MERESQPARTGLTQTLALNNDIWRVSFYRFCQLLELENPDGPKL
                     GTTSHPGDDPVRFRPWPGMGFPVSTLKAVEIDEDRPTLPPTIRTTFLGMYGVDSPLPT
                     SWLDDITQR"
     gene            complement(296..>1843)
                     /gene="tssF"
                     /locus_tag="ACHKIR_005238"
     CDS             complement(296..>1843)
                     /gene="tssF"
                     /locus_tag="ACHKIR_005238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009309580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFO9992821.1"
                     /translation="LVSLLWPHYMRTIPSLAIVEFSPDWRSLRQAEILAEGFSVLSRP
                     VGPHKTACQYRTTRDVLLQPLHLADARLHTESDGRSAIRLRFECPEKVDWSKAGIDKV
                     AIFLNAEAPVSAALHLAMTRRVHAMYARHAGTYTGRHQFDGWCRPMGFDDNDCLWKKA
                     DTAFSGYQLLLEYFSFRPKFMFVELRGLDTIGLTAGSTWFEIDIVLSEAWSSGLPFET
                     ENFRLHCAPVINLFTLEADPLILNPLDNEYLLRPLRLQDGHTEIYSVDNIHGSVKNGR
                     HPYVPFTSFRHRGGMMRHDAPERYYHTRVKRGPSGLYDTWLVLGGRSFELEQLSDKPE
                     SLSMRITATNGQLPRRALESTLLDRVVKTGKVPVRVMNLTAPTMPLYPPANDRFHWRV
                     MSHLGSNFLSMMDNPEVLRGTLALYDWTDDEMNRRRLEAIVAVKHTLIRRFEKGFMLR
                     GVDIEVTLNMDNFAGEGDVNLFGEMLHRFFALYADVHLFNQLTLVLQPTGKRLRWKEN
                     HNLHVPG"
BASE COUNT          414 a          512 c          531 g          386 t
ORIGIN      
        1 cgctgcgtaa tatcatccag ccaggacgtc ggcagaggtg agtccacccc atacattccg
       61 agaaatgtcg tgcggattgt tgggggtaat gtcggacggt cttcgtcgat ctccaccgct
      121 ttcagggtgc tgaccgggaa tcccatcccg ggccagggtc tgaatcgcac cggatcatca
      181 cccggatggc tggtcgtgcc cagttttggc ccgtccggat tttccagctc cagcagctgg
      241 cagaaacgat aaaaactgac ccgccagata tcgttgttaa gagccagggt ctgcgtcagc
      301 ccggtacgtg caggttgtga ttctctttcc atctcagtcg tttccctgtg ggttgcagta
      361 ccagtgtgag ctggttgaaa aggtggacat cagcgtagag cgcaaagaaa cggtgcagca
      421 tctccccgaa gagattcaca tcgccctcgc cggcaaaatt atccatattc agcgtgacct
      481 caatgtccac gccacggagc ataaagccct tttcaaaacg gcggatcagg gtgtgcttca
      541 cggcaacgat ggcttcaagc cggcggcggt tcatttcatc gtcggtccag tcatagagcg
      601 ccagcgtccc ccgcagcacc tccggattgt ccatcatgct gagaaagttt gatccgaggt
      661 gactcatcac ccgccagtga aaccggtcat ttgccggagg atacagtggc atcgtcggtg
      721 cggtgaggtt catgactctg acggggactt tgccggtttt gaccaccctg tccaggagcg
      781 tactttccag cgccctgcgc ggaagctgcc cgttggtggc ggtgattcgc atcgagaggg
      841 actccggttt atcagacagc tgctccagtt cgaacgagcg tcccccgaga actaaccatg
      901 tatcatacag accggagggg ccccgtttca cgcgggtgtg gtaataacgt tcaggagcat
      961 cgtggcgcat cataccgccc cgatgccgga agctggtgaa cgggacataa gggtgtctgc
     1021 cattcttcac cgagccgtga atgttatcga cgctgtaaat ttcggtgtga ccatcctgaa
     1081 gacgcagcgg cctgagcagg tactcgttat ccagcgggtt aaggatcagc gggtcggctt
     1141 ccagggtaaa gagattgata accggcgcac agtgcaggcg gaagttttct gtctcaaaag
     1201 gcaaaccgga tgaccaggct tcactgagca caatgtcgat ttcaaaccag gtactgcccg
     1261 ccgtgagccc gatggtgtcg aggccgcgca gttcaacaaa cataaacttc ggacggaagc
     1321 tgaaatactc cagcagcaac tggtaaccac tgaaggcagt atcggctttc ttccacaggc
     1381 agtcattgtc gtcaaagccc atgggtctgc accagccgtc aaactgatga cgtccggtat
     1441 atgtcccggc atgacgcgca tacatggcat gtacacgccg ggtcattgcc agatgcagag
     1501 cagcgcttac cggcgcctct gcgttaagaa aaatggcgac tttgtcgatc ccggctttac
     1561 tccagtccac tttttcaggg cattcgaagc gcaggcggat agcagagcgg ccatcggatt
     1621 cagtatggag acgggcatct gcaaggtgca gcggttgtag caacacatcg cgggtcgtcc
     1681 ggtactgaca ggcggtttta tgcggcccga caggacggga caaaacggag aagccttcag
     1741 ccagtatttc ggcctgacgc agactacgcc agtcaggcga gaactcaacg atggccagcg
     1801 acggaatagt ccgcatatag tgcggccaca gcaggctcac cag
//
LOCUS       DAZGQL010000266         1843 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.268, whole genome shotgun sequence.
ACCESSION   DAZGQL010000266 DAZGQL010000000
VERSION     DAZGQL010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.268"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..332)
                     /locus_tag="ACHKIR_005239"
     CDS             complement(<1..332)
                     /locus_tag="ACHKIR_005239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002445141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFO9992822.1"
                     /translation="MERESQPARTGLTQALALNNDIWRASFYRFCQLLEQENPDGPKL
                     GTTSHPGDDPVRFRPWPGMGFPVSTLKAVEIDEDRPTLPPTIRTTFLGMYGVDSPLPT
                     SWLDDITQR"
     gene            complement(296..>1843)
                     /gene="tssF"
                     /locus_tag="ACHKIR_005240"
     CDS             complement(296..>1843)
                     /gene="tssF"
                     /locus_tag="ACHKIR_005240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009309580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFO9992823.1"
                     /translation="LVSLLWPHYMRTIPSLAIVEFSPDWRSLRQAEILAEGFSVLSRP
                     VGPHKTACQYRTTRDVLLQPLHLADARLHTESDGRSAIRLRFECPEKVDWSKAGIDKV
                     AIFLNAEAPVSAALHLAMTRRVHAMYARHAGTYTGRHQFDGWCRPMGFDDNDCLWKKA
                     DTAFSGYQLLLEYFSFRPKFMFVELRGLDTIGLTAASTWFEIDIVLSEAWSSCLPFET
                     ENFRLHCAPVINLFTLEADPLLLNPLDNEYLLRPLRLQDGHTEIYSVDNIHGSVKNGR
                     HPYVPFTSFRHRGGMMRHDAPERYYHTRVKRGPSGLYDTWLVLGGRSFELEQLSDKPE
                     SLSMRITATNGQLPRRALESTLLDRVVKTGKVPVRVLNLTAPTMPLYPPANDRFHWRV
                     MSHLGSNFLSMMDNPEVLRGTLALYDWTDDEMNRRRLEAIVAVKHTLIRRFEKGFMLR
                     GVDIEVTLNMDNFAGEGDVNLFGEMLHRFFALYADVHLFNQLTLVLQPTGKRLRWKEN
                     HNLHVPG"
BASE COUNT          412 a          511 c          535 g          385 t
ORIGIN      
        1 cgctgcgtaa tatcatccag ccaggacgtc ggcagaggtg agtccacccc atacattccg
       61 agaaatgtcg tgcggattgt tgggggtaat gtcggacggt cttcgtctat ctccaccgct
      121 ttcagggtgc tgacagggaa tcccatcccg ggccagggcc tgaatcgcac cggatcatcg
      181 cccggatggc tggtcgtgcc cagttttggc ccgtccggat tttcctgctc cagcagctgg
      241 cagaaacgat aaaaactggc ccgccagata tcgttgttaa gggccagggc ctgcgtcagc
      301 ccggtacgtg caggttgtga ttctctttcc atctcagtcg tttccctgtg ggttgcagta
      361 ccagtgtgag ctggttgaaa aggtggacat cagcgtagag cgcaaagaaa cggtgcagca
      421 tctcgccgaa gagattcaca tcgccctcgc cggcaaaatt atccatattc agcgtgacct
      481 caatgtccac gccacggagc ataaagccct tttcaaaacg gcggatcagg gtgtgcttca
      541 cggcaacgat ggcttcaagc cggcggcggt tcatttcatc gtcggtccag tcatagagcg
      601 ccagcgtccc ccgcagcacc tccggattgt ccatcatgct gagaaagttt gaaccgaggt
      661 gactcatcac ccgccagtga aaccggtcat ttgccggggg atacagtggc atcgtcggtg
      721 cggtcaggtt caggactctg acggggactt tgccggtttt gaccaccctg tccaggagcg
      781 tactttccag cgccctgcgc ggaagctgcc cgttggtcgc ggtgattcgc atcgagaggg
      841 actccggttt atcagacaac tgctccagtt cgaacgaacg tcccccgaga actaaccatg
      901 tgtcatacag gccggagggt ccccgtttca cgcgggtgtg gtaataacgt tcaggagcat
      961 cgtggcgcat cataccgccc cggtgccgga agctggtgaa cgggacataa gggtgtctgc
     1021 cattcttcac cgagccgtga atgttatcga cgctgtaaat ttcggtgtga ccatcctgaa
     1081 gacgcagcgg cctgagcagg tactcgttat ccagcgggtt aaggagcagc gggtcggctt
     1141 ccagggtaaa gagattgata accggcgcac aatgcaggcg gaagttttct gtctcaaaag
     1201 gcaaacagga tgaccaggct tcactgagca caatgtcgat ttcaaaccag gtactggccg
     1261 cagtgagccc gatggtgtcg aggccgcgca gttcaacaaa cataaacttc ggacggaagc
     1321 tgaaatactc cagcagcaac tggtaaccac tgaaggctgt atcggctttc ttccacaggc
     1381 agtcattgtc gtcaaagccc atgggtctgc accagccgtc aaactgatga cgtccggtat
     1441 atgtcccggc atgacgcgca tacatggcat gtacacgccg ggtcattgcc agatgcagag
     1501 cagcgcttac cggcgcctct gcattaagaa aaatggcgac tttgtcgatc ccggctttac
     1561 tccagtccac tttttcaggg cattcgaagc gcaggcggat ggcagagcgg ccatcggatt
     1621 cagtatggag acgggcatct gcaaggtgca gcggttgtag caacacatcg cgggtcgtcc
     1681 ggtactgaca ggcggtttta tgcggcccga caggacggga caaaacggag aagccttcag
     1741 ccagtatttc ggcctgacgc agactacgcc agtcaggcga gaactcaacg atggccagcg
     1801 acgggatagt ccgcatatag tgcggccaca gcagactcac cag
//
LOCUS       DAZGQL010000267         1813 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.269, whole genome shotgun sequence.
ACCESSION   DAZGQL010000267 DAZGQL010000000
VERSION     DAZGQL010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1813
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.269"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(395..562)
                     /locus_tag="ACHKIR_005241"
                     /pseudo
     CDS             complement(395..562)
                     /locus_tag="ACHKIR_005241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415891.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            712..1380
                     /locus_tag="ACHKIR_005242"
     CDS             712..1380
                     /locus_tag="ACHKIR_005242"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO9992824.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     INHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGIVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            1746..>1813
                     /locus_tag="ACHKIR_005243"
     CDS             1746..>1813
                     /locus_tag="ACHKIR_005243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992825.1"
                     /translation="MNSILIITSLLIIFSIFSHALI"
BASE COUNT          576 a          388 c          289 g          560 t
ORIGIN      
        1 ttattatatg tatattgttt tttattctaa cgtatttcaa agttacattt ttcaacgctt
       61 actatacttt ttattaacat aaactcacta caacgcacct gaaacctctt gctatatata
      121 tgtcaaccgt ttgaatttaa aataaaaaga gtatcatttt tacttgcatt tcttatcaag
      181 tcacattcaa caacagtaaa aaaacattat tagaaccatt caattaacaa aaaaaccaac
      241 atccagcttg cttaattttt ctttattaaa cgatattgaa aatcaattga taaaatacat
      301 ctaaacaacc ttttggggcg caaaagcata acatcaaaca aataacacac cgaaaaaact
      361 cacaattaat aacctatgat atacatactg tttattatgg ttgaataagc cactcgatat
      421 ctggtgctac ggaagtgtcc acacggttta gcagcacccg atactttttt caggcttcca
      481 gcaacgatct ttcttcctcc gttgcgattt ccagatctac agcatcctgc agtggcgcaa
      541 tatactcact gaattcctgg atgtagaact gtgtggtgac ggtcttccag ctattcggct
      601 cctgctgtat tgaagcatac caggctattt caatatcgct atgctgcggc agcatttaac
      661 cccttgtaat tcattgccat aattgattta attcacaaat aaaactataa catggtgaaa
      721 ttaatgaaaa aaaacacaga tgatggggct aaaatttaca caccacttac cctaaagctt
      781 tatgactggt gggttttggg agtatcaaat cggcttgcat ggggatgtcc tacaaaggaa
      841 caccttcttc cacactttct ggaacattta ggtaacaacc atctggatat tggtgttgga
      901 actgggtttt accttactca cgtacctgag agtagtctga tatctttaat ggatttgaac
      961 gaagctagcc tgaacgcggc atctacaagg gctggggaat caaaaattaa acataaaatt
     1021 aaccatgatg tttttgaacc ttatcccgcg gcgttacatg gtcaatttga ttccatttcc
     1081 atgttttacc ttcttcactg cctgcctgga aatatatcta caaaaagctg tgtaatacgc
     1141 aatgcggcgc aggccttaac tgacgatgga actctatacg gagccacaat tcttggcgat
     1201 ggaattgtgc acaatagctt cggtcaaaaa ctgatgcgca tttacaatca gaaaggcatc
     1261 ttttcaaaca caaaagattc cgaagaaggc ttaacacata tactctcaga gcatttcgag
     1321 aatgttaaaa ccaaggttca aggtactgta gtaatgtttt ccgcttcagg gaaaaaatag
     1381 catccaaccg cagcacgttc ttgcataagg cgtgtctgcg gtttttcaac tattcagata
     1441 catcactccc atcacattca ttcctccgca tcataggcat ataggctata tcaccttgat
     1501 atttttcttc ttcagataaa aactgttatc tatgtatact tttaaaccca atccgtgtag
     1561 agtctctgca taagatagtt tgcagttgac acttcagctt gcgccataaa ccgcctgatt
     1621 tttgccgcca cctgttagca ttcctgtata cctgaaacga caatgtttat ctacgaactt
     1681 taagaacacc caggataaaa attgtcaact atatcatata taacacatta ctaattcgag
     1741 gctatatgaa cagcatactg ataatcactt cgctccttat catattcagc atttttagtc
     1801 atgctctaat aaa
//
LOCUS       DAZGQL010000268         1781 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.270, whole genome shotgun sequence.
ACCESSION   DAZGQL010000268 DAZGQL010000000
VERSION     DAZGQL010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.270"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..543)
                     /locus_tag="ACHKIR_005244"
     CDS             complement(<1..543)
                     /locus_tag="ACHKIR_005244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO9992826.1"
                     /translation="MTQTESAILVHARRCAPAESCGFVISTPEGERYQPCMNISAEPE
                     AYFRITPEDWLQAQMQGEIVALVHSHPGGLPWLSEADRRLQIKSALPWWLVCRGEIHR
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGITLPDFVREDDWWRNGQNLYLDNLAEN
                     GFYRVSPSCAQAGDILLCCFG"
     gene            complement(554..1252)
                     /locus_tag="ACHKIR_005245"
     CDS             complement(554..1252)
                     /locus_tag="ACHKIR_005245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO9992827.1"
                     /translation="MQDIHEESLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEPVTWQGRKYEAYPIEGSGFEMNGKGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELTDRWVVEQMSSLTAMTASFVLATPTETDG
                     ALFPGRIMLANTCMWDYRGDECGYNGPAVADEFDNPTTDIRKDRCSKCMRGCEMRGMV
                     ANFGGFLSINKLSQ"
     gene            complement(1252..1581)
                     /locus_tag="ACHKIR_005246"
     CDS             complement(1252..1581)
                     /locus_tag="ACHKIR_005246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9992828.1"
                     /translation="MKTFRWKVKPDMEVNSQPSVREVRFGDGYSQRMAAGLNADLKTY
                     RVTLSVTREEARHLEAFLAEHGGWKAFLWKPPYAYRQIKVTCAGWSARVGMLRVEFSA
                     EFKQVVN"
     gene            complement(1578..>1781)
                     /locus_tag="ACHKIR_005247"
     CDS             complement(1578..>1781)
                     /locus_tag="ACHKIR_005247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO9992829.1"
                     /translation="AGSPAQMRRAEGINFNQNNHVVIQNDGINGQAGPQLMKAVYDMA
                     RKGAQDELRLQLRDGGMLSGSGR"
BASE COUNT          368 a          637 c          421 g          355 t
ORIGIN      
        1 gccgaagcag cacagcagga tatcgcccgc ctgtgcacag gacggagaca cccggtaaaa
       61 gccgttttcc gccaggttgt ccaggtacag gttctgaccg ttgcgccacc agtcatcctc
      121 acgcacaaaa tccggcagcg ttatccccgc cagatggtat gcatcccgga acagggtgta
      181 acagtccgtc acaccgtgtt caaagcgccg tccggtcagg tgcggcacac agcggaaccg
      241 gtgaatttca ccccggcaga ccagccacca gggcagggca ctctttatct gcagccgccg
      301 gtccgcctcg ctcagccagg gcagaccacc gggatgactg tggaccagcg ccacaatctc
      361 cccctgcatc tgtgcctgca gccagtcttc cggtgtaata cgaaaatacg cctccggctc
      421 tgcggagata ttcatgcagg gctggtaccg ttcgccctcc ggggtgctta tcacgaagcc
      481 gcacgactcc gctggcgcac accgccgggc atgcaccaga atcgctgatt cagtctgtgt
      541 cataaaacgg gatttactgc gaaagtttat tgatggaaag gaaaccgcca aaattagcca
      601 ccatgccgcg catctcacac ccgcgcatgc atttactgca tctgtccttc cggatatccg
      661 tggtggggtt gtcgaactca tccgccactg ccggaccgtt atacccgcac tcatcgcccc
      721 ggtaatccca catacaggtg ttcgccagca tgatgcgacc gggaaacagc gcaccgtccg
      781 tctccgtcgg tgtcgccagc acaaacgagg ccgtcatggc cgtcagcgat gacatctgct
      841 ccaccaccca ccggtccgtc agctcctgct ccgggtctgc ctcaggattg cctgccacaa
      901 aattcaccgc atccagaaaa cgcgcataca cccggcgacg gaccaccgtg gcacccacca
      961 ggctctgcaa atcctccgcc atcccggtga ccagtccgaa aagattggac accgtcagcg
     1021 acgggcgggc actgctgccc tttccgttca tctcaaagcc gctgccctca atcgggtacg
     1081 cctcatactt ccgcccctgc caggtcaccg gctccccttt ttcattcagc tcattgcaga
     1141 aaaaataccg ctcaccgccc tgcaccgtca ggtcgatttc ccagagcacc acccgcggtg
     1201 actgctctga cttaaccgac tcgttcaggc tttcttcgtg aatatcctgc atcagttcac
     1261 cacctgctta aactccgcgc tgaactcaac gcgcaacatc ccgacccgcg cagaccaccc
     1321 ggcgcaggtc acctttatct gccggtatgc atagggtggc ttccacaaaa atgccttcca
     1381 gcccccgtgc tctgccagaa acgcttccag atgccgggcc tcctcccggg tcacggaaag
     1441 cgtcacccgg tatgttttca ggtcagcatt cagccctgcc gccatacgct gtgagtaccc
     1501 gtcaccaaaa cgcacttcac gcaccgacgg ctgcgagttc acctccatat ccggcttcac
     1561 tttccagcga aaggttttca tcgcccgctc cctgataaca taccgccatc acgcaactgc
     1621 agccggagtt catcctgcgc ccccttacgg gccatgtcat acaccgcctt catcagctgc
     1681 ggccccgcct gtccgtttat accgtcgttc tgaatcacca cgtgattgtt ctgattaaaa
     1741 ttaatacctt ccgcccgccg catctgcgcc ggacttccgg c
//
LOCUS       DAZGQL010000269         1762 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.271, whole genome shotgun sequence.
ACCESSION   DAZGQL010000269 DAZGQL010000000
VERSION     DAZGQL010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1762
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.271"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            187..1131
                     /locus_tag="ACHKIR_005248"
     CDS             187..1131
                     /locus_tag="ACHKIR_005248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992830.1"
                     /translation="MSPDIISSAVNRFSGWPSNDPLKTDKESMTSPMTVQVIKMIAAI
                     LDPHRIVVGIDTLYTTQRETFTSESQQLIESALQTLHVMNLNNLETNLDEEGHYLAFG
                     YNVDTPDRITVSAYNEKKPGCYGQCLLIQDDETDLFNLIKVKIFEARHNYVAQLMSDP
                     EFMFKFSYSAQQNRLEPHLLVPTYFPLKTNEPVTQEDVLLLYRFFKMDGDFNILTSDE
                     YMSIMTPLMKCERSIHDHNRYVTGKDTLLLDYPPSGNQIYFHIFPDESASLVLYFSNN
                     DIECFVFERDIPSQYRFFKMFTNLALVIDQLTDANQIL"
     gene            1212..1634
                     /locus_tag="ACHKIR_005249"
     CDS             1212..1634
                     /locus_tag="ACHKIR_005249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992831.1"
                     /translation="MIPSQISFNTLPVNATYASEPTVDTQKFSDILYSAGCSLKDLAY
                     LSRCLASIHPTIAKNIYETENLSDQKLLHIDCRSTNEIKINIFFGQQREGLIEINSDT
                     VIFSILSSLIDSTISKFQHQNPVNGSVNPDLLYEDYTD"
BASE COUNT          558 a          365 c          298 g          541 t
ORIGIN      
        1 ttgtttcatt agtaataatc acttaaatgt atcgatgaaa caggtatgac ttagcgggag
       61 tgattgtttc tgagtgaaca gagatcactt tcgcctgata aatgaaaaat gagcgatttt
      121 tgcaagttag tttttctata gccgagtacg ctcagcgcct cgcttgatta caatgaggtt
      181 gctgctatga gtcctgatat tatcagttct gctgtcaatc gcttctcagg ttggccatct
      241 aatgatcctc taaaaacaga taaagaatca atgacatcac ccatgactgt gcaggtaata
      301 aagatgattg ccgccatcct tgacccacat cggatagtcg ttggaatcga tacactttat
      361 acaacgcagc gcgaaacatt tacctcagag agtcaacagc tcattgaatc agcattgcaa
      421 acacttcatg tgatgaattt aaataacctg gaaacaaatc tcgatgagga aggtcactat
      481 ctggcttttg gatataacgt tgacactcct gacagaataa ccgtttctgc ttataatgaa
      541 aaaaagcctg gctgctatgg ccaatgtctt ttaatacagg atgacgaaac agacttattt
      601 aacctgataa aagttaaaat ttttgaagcc aggcataatt atgtcgctca attgatgagc
      661 gatccggaat ttatgttcaa atttagttat agcgctcaac aaaatagatt agaacctcat
      721 cttctggttc ccacttattt ccctctaaaa acaaacgaac cggttacgca agaggatgta
      781 ctgttacttt atcgtttctt taaaatggat ggtgatttta atatattaac atcagatgaa
      841 tacatgagta tcatgacacc tctgatgaag tgtgagcgca gcatccacga ccacaacagg
      901 tatgtgacgg gcaaagatac ccttcttctg gactatcctc cttctggtaa ccagatatat
      961 ttccatattt ttcctgacga atcagcatca ctagtccttt atttttcaaa caatgacatt
     1021 gaatgtttcg tctttgaaag agatattccg agccagtatc gctttttcaa aatgtttacc
     1081 aatctggcac tggttattga tcagttaacc gatgccaatc aaatacttta atttttatat
     1141 atcccctgtg gcaaatatta ctaaacaatg cccggggatt aatttttatt actcacggaa
     1201 agatttaaat aatgatccca tctcaaatct ccttcaacac ccttccagtc aatgcaacat
     1261 acgcttcaga gccaacagtt gacacacaaa aattctctga catcctctac tcagcggggt
     1321 gttccctaaa ggatctggct tatctgagca gatgtctggc atcaatacat ccgactatcg
     1381 ccaaaaacat ttatgaaaca gaaaatctca gcgatcaaaa attactacat attgattgcc
     1441 gtagtacaaa tgaaatcaag ataaatatct tttttggtca acaacgagaa ggattaattg
     1501 aaattaattc tgacacagtc attttttcga tactgtcttc attgattgac tccaccattt
     1561 ctaaatttca acaccaaaac cccgttaacg gttctgtaaa tcctgaccta ttgtatgagg
     1621 attatactga ttaaatccct cctgtcgcga aaaatggcct gcgttattgc cagacgtaat
     1681 aaaacttttg attaacgttt ctcgtactgg tagcaccttc atgtactttt cttttttacg
     1741 aaaaatcaaa agagctaaca aa
//
LOCUS       DAZGQL010000270         1719 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.272, whole genome shotgun sequence.
ACCESSION   DAZGQL010000270 DAZGQL010000000
VERSION     DAZGQL010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1719
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.272"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..474
                     /locus_tag="ACHKIR_005250"
     CDS             <1..474
                     /locus_tag="ACHKIR_005250"
                     /inference="COORDINATES: protein motif:HMM:NF026977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992832.1"
                     /translation="PLGLLGGEQTYRYVPNPCGYVDPLGLAITSIINKTVEFSRGPQT
                     YVSDLGHVTGSTARARNKAITAILKEDFAYLKLTYIPQYNPFMRTGIAKLWEGTQFGK
                     NSFSSRAALRDVIIHEELHHRWWKRGLNDHHPKGTEMEQKFYETIRRYKRMRGWL"
     gene            474..887
                     /locus_tag="ACHKIR_005251"
     CDS             474..887
                     /locus_tag="ACHKIR_005251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992833.1"
                     /translation="MFGDEFKTTIECPCCGKICSIAIGQTSHNGILAWYESIICHNCG
                     LRSESDDIGFPPEKIRLQLIDFEGLWNVRLENIKSQMETIKVIRKAFSIDTKEALRII
                     RNESKVIYSGTREEAIWLMSLLKHVGETPLIQIAR"
     gene            942..1103
                     /locus_tag="ACHKIR_005252"
                     /pseudo
     CDS             942..1103
                     /locus_tag="ACHKIR_005252"
                     /inference="COORDINATES: protein motif:HMM:TIGR01643.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat domain-containing protein"
     gene            1134..>1719
                     /locus_tag="ACHKIR_005253"
     CDS             1134..>1719
                     /locus_tag="ACHKIR_005253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
                     /protein_id="HFO9992834.1"
                     /translation="MNVTTQGRRAIEYHYPDEHTVIRCILPPEDERDRHPGESLLKTT
                     YRYNAAGELTEVILPGDETLTFSRDEAGREVLRHSNRGFACEQGWNAASQLVTQRAGF
                     FPEETTWGGLLPSLVREYRYDSAGNVSAVTSREDYGRETRREYRLDRNGQVTAVTASG
                     TGLGYGEGDESYGYDSCGYLKAQSAGRHRISEETD"
BASE COUNT          478 a          374 c          474 g          393 t
ORIGIN      
        1 ccgctggggt tgctgggggg tgagcagact taccggtatg tgcctaatcc gtgtgggtat
       61 gttgatccgc tgggattagc tataactagt ataattaata aaacagtcga gttttctaga
      121 gggccacaga cttatgtttc tgatctcggt catgttacag gttctacggc cagagccaga
      181 aataaggcaa ttacagccat acttaaagaa gattttgcat atcttaaact aacatatatc
      241 cctcaataca atccgtttat gcgaacaggg attgctaaac tttgggaagg aactcagttt
      301 ggtaaaaata gtttttcgtc tcgtgcagca ttaagagatg ttattattca tgaagaatta
      361 catcataggt ggtggaagcg aggattgaat gatcatcatc ccaaaggtac tgagatggaa
      421 cagaaatttt acgagacgat tcgaagatat aagcggatga gagggtggtt atagtgtttg
      481 gtgatgaatt taaaacgact attgagtgtc cttgctgtgg aaaaatatgt tctatagcta
      541 tagggcagac gagtcacaat ggtatactcg catggtatga atcaattata tgccataatt
      601 gcggattacg tagtgaatca gatgatatag gctttccccc agaaaaaatt cgtctacaat
      661 tgattgattt tgagggttta tggaatgtta gacttgaaaa tataaaatca cagatggaaa
      721 ctataaaagt cattcgtaag gctttttcga ttgatacaaa agaagcactt cgaattatta
      781 gaaatgaatc gaaggtaata tactccggaa cgagagaaga ggccatctgg ttaatgagtt
      841 tattaaaaca tgtgggtgaa acccccttaa tacaaattgc tagataaatt ctatgagctg
      901 ggtacaacgg agtagcctgg cgttaccgct atgacgccga tggcctgtgt accgccaggg
      961 ttaacggtct ggaggaaacc atcctctaca gccgggacgc cgcaggccgc ctggcagaag
     1021 tcatcacgcc ggaaggcaaa acgcagtatg cgtatgacaa atccggcagg ctgacgggta
     1081 tcttcagccc ggacggcaca tcacagcgca ccggctatga cgaacgcggg cgggtgaatg
     1141 tcaccactca gggccgacgg gccattgaat accactaccc cgatgaacac accgtcatcc
     1201 gctgtatcct gccaccggaa gatgaacgcg acagacatcc cggtgaatcc ctgctgaaaa
     1261 ccacctaccg ctacaacgcc gccggagaac tgacggaggt tatcctgccg ggggatgaga
     1321 cgctgacgtt cagccgtgat gaggcaggac gtgaagtgct ccggcacagt aaccggggtt
     1381 ttgcctgtga gcagggctgg aatgcagcca gccagcttgt cacccagcgc gccggatttt
     1441 tcccggagga aaccacatgg ggcgggctgc tcccctcact ggtacgggag taccgttacg
     1501 acagcgcggg caatgtgtcg gctgtcacca gccgggaaga ttacggacgg gaaacacggc
     1561 gggaataccg gctggaccgg aacggtcagg tcacggcggt gacagcctca ggcaccgggc
     1621 tgggctatgg cgaaggcgat gagtcctatg gctatgacag ttgtggctac ctgaaggcgc
     1681 agtctgcggg caggcaccgg ataagtgaag agactgacc
//
LOCUS       DAZGQL010000271         1714 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.273, whole genome shotgun sequence.
ACCESSION   DAZGQL010000271 DAZGQL010000000
VERSION     DAZGQL010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1714
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.273"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..476)
                     /locus_tag="ACHKIR_005254"
     CDS             complement(<1..476)
                     /locus_tag="ACHKIR_005254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO9992835.1"
                     /translation="MTQTESAILVHARRCAPAESCGFVISTPEGERYQPCMNISAEPE
                     AYFRIAPEDWLQAQMQGEIVALVHSHPGGLPWLSEADRRLQIKSALPWWLVCRGEIHR
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGITLPDFVREDDWWRNGQNLYLDNLA"
     gene            complement(487..1185)
                     /locus_tag="ACHKIR_005255"
     CDS             complement(487..1185)
                     /locus_tag="ACHKIR_005255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO9992836.1"
                     /translation="MQDIHEASLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEPVTWQGREYQAYPIEGSGFEMNGKGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELTDRWVVEQMSSLTAMTASFVLATPTETDG
                     ALFPGRIMLANTCMWDYRGDECGYNGPAVADEFDNPTTDIRKDRCSKCMRGCEMRGMA
                     VNFGGFLSINKLSQ"
     gene            complement(1185..1514)
                     /locus_tag="ACHKIR_005256"
     CDS             complement(1185..1514)
                     /locus_tag="ACHKIR_005256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9992837.1"
                     /translation="MKTFRWKVKPDMEVNSQPSVREVRFGDGYSQRTAAGLNADLKTY
                     RVMLSVTREEARHLEAFLAEHGGWKAFLWKPPYAYRQIKVTCAGWSARVGMLRVEFSA
                     EFKQVVN"
     gene            complement(1511..>1714)
                     /locus_tag="ACHKIR_005257"
     CDS             complement(1511..>1714)
                     /locus_tag="ACHKIR_005257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO9992838.1"
                     /translation="AGSPAQMRRVEGINFNQNNHVVIQNDGINGQAGPQLMKAVYDMA
                     RKGAQDELRLQLRDGGMLSGSGR"
BASE COUNT          351 a          617 c          403 g          343 t
ORIGIN      
        1 tccgccaggt tgtccaggta caggttctgg ccgttgcgcc accagtcatc ctcacgcaca
       61 aaatccggca gcgttatccc cgccagatgg tatgcatccc ggaacagggt gtaacagtcc
      121 gtcacaccgt gttcaaagcg ccgtccggtc aggtgcggca cacagcggaa ccggtgaatt
      181 tcaccccggc agaccagcca ccagggcagg gcactcttta tctgcagcct ccggtccgcc
      241 tcgctcagcc agggcagacc accgggatga ctgtggacca gcgccacaat ctccccctgc
      301 atctgtgcct gcagccagtc ttccggtgca atacgaaaat acgcctccgg ctctgcggag
      361 atattcatgc agggctggta ccgttcgccc tccggggtgc ttatcacgaa gccgcacgac
      421 tccgctggcg cacaccgccg ggcatgcacc agaatcgctg attcagtctg tgtcataaaa
      481 cgggatttac tgcgaaagtt tattgatgga aaggaaaccg ccaaaattga ccgccatgcc
      541 gcgcatctca cacccgcgca tgcatttact gcatctgtcc ttccggatat ccgtggtggg
      601 gttgtcgaac tcatccgcca ctgccggacc gttatacccg cactcatcgc cccggtaatc
      661 ccacatacag gtgttcgcca gcatgatgcg accgggaaac agcgccccgt ccgtctccgt
      721 cggtgtcgcc agcacaaacg aggccgtcat ggccgtcagc gatgacatct gctccaccac
      781 ccaccggtcc gtcagctcct gctccgggtc ggcctcagga ttgcctgcca caaaattcac
      841 cgcatccaga aaacgcgcat acacccggcg acggaccacc gtggcaccca ccaggctctg
      901 caaatcctcc gccatcccgg tgacaaggcc gaaaagattg gagaccgtca gcgacgggcg
      961 ggcactgctg ccctttccgt tcatctcaaa gccgctgccc tcaatcgggt acgcctgata
     1021 ttcacgcccc tgccaggtca ccggctcccc tttttcattc agctcattgc agaaaaaata
     1081 ccgctcaccg ccctgcaccg tcaggtcgat ttcccagagc accacccgcg gtgactgctc
     1141 tgacttaacc gactcgttca ggcttgcttc gtgaatatcc tgcatcagtt caccacctgc
     1201 ttaaactccg cgctgaactc aacacgcaac atcccgaccc gcgcagacca cccggcacag
     1261 gtcaccttta tctgccggta tgcatagggt ggcttccaca aaaatgcctt ccagcccccg
     1321 tgctctgcca gaaacgcttc cagatgccgg gcctcctccc gggtcacgga aagcatcacc
     1381 ctgtacgtct tcaggtcagc attcagccct gccgccgttc gctgcgaata cccgtcacca
     1441 aaacgcactt cacgcaccga cggctgcgag ttcacctcca tatccggctt cactttccag
     1501 cgaaaggttt tcatcgcccg ctccctgata acataccgcc atcacgcaac tgcagccgga
     1561 gttcatcctg cgcccccttg cgggccatgt catacaccgc cttcatcagc tgcggccccg
     1621 cctgtccgtt tataccgtcg ttctgaatca ccacgtgatt gttctgatta aaattaatac
     1681 cttccacccg ccgcatctgc gccggacttc cggc
//
LOCUS       DAZGQL010000272         1713 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.274, whole genome shotgun sequence.
ACCESSION   DAZGQL010000272 DAZGQL010000000
VERSION     DAZGQL010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1713
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.274"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..668
                     /locus_tag="ACHKIR_005258"
     CDS             <1..668
                     /locus_tag="ACHKIR_005258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002431274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oligogalacturonate-specific porin KdgM family
                     protein"
                     /protein_id="HFO9992839.1"
                     /translation="PLCSQALTLNLRGGYRSASHAYETLIQVSQGWNNGWWASFESNT
                     WNTIHNNENELFALNYNKIDINKTYKIDDKWALIPGIQTQWMSAGSQYNPYLKISYQA
                     TPDLNLALRYRYDWKAFRQTDLDGNRARNDQHQFDGYVTYKVNDSWLFAWQTTVYTKV
                     NNFKYGNHKKTATENAFVLQYKMSPVFTPYIEYDYLDKQGVYKGKDNKHENSYRVGVL
                     INF"
     gene            681..>1713
                     /locus_tag="ACHKIR_005259"
     CDS             681..>1713
                     /locus_tag="ACHKIR_005259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HFO9992840.1"
                     /translation="MKVKNFIYLPFCLFIGTSVAGALPEIPEPFKYGVGGIDNGKIYI
                     GLGSLGNNWYMIDTNQSEKKWTKIAQWPTVPREQATATIIDGKIYVFGGIGKDKSGVI
                     TLQKDVYSYDIAKDKWEKLMTRPPVSLAGHVSFIHNGHAVSTGGVNENIFNGYFSDVE
                     LSKGNSALTEKVNRDYFSKPVDDYFLNNHMISYDPSKNQWKNLGTTPFPGTAGSSVIF
                     AEQQIYILGGERKPGLRSVRSWTGELSHDRIKWSELPPVASPEGVSGAYASVIDGNIF
                     LAGGAYFPGAAEKYSNGEYWSHKGLDKAYSKEIYQLIKNDWKKVGSLPEGLAYGVSLP
                     WQGGMLILGG"
BASE COUNT          553 a          275 c          401 g          484 t
ORIGIN      
        1 tacctttgtg ctctcaggca ttaactctta atctacgtgg aggatacaga agcgccagtc
       61 acgcttatga aacgttgatt caggttagtc aggggtggaa taatggttgg tgggcaagtt
      121 ttgagtcaaa tacctggaat acaattcaca acaatgaaaa tgaattgttt gcactaaatt
      181 ataataaaat agatataaat aaaacatata aaatagatga caaatgggct ctaatcccag
      241 ggattcagac acaatggatg tcagcaggaa gtcagtataa cccttatctg aaaatttcgt
      301 atcaggcaac gccagatctt aatctggcct tacgctatcg ctatgactgg aaagctttcc
      361 gacagacgga tttagatggc aacagggcgc gtaatgatca gcatcagttt gatggttatg
      421 tgacatacaa agttaatgat tcatggttgt ttgcatggca gaccacggta tatacgaagg
      481 ttaataattt caaatatggg aatcataaaa aaacagcaac tgaaaatgca tttgttctgc
      541 aatataaaat gtcaccggtg tttaccccat atatagaata tgattacctg gataaacaag
      601 gggtgtataa agggaaagat aataaacatg aaaatagtta ccgtgtcggt gttttgatta
      661 atttttaaaa ggtggttaca atgaaagtga aaaactttat atatcttccc ttctgcttgt
      721 ttattggaac gtcggtagcc ggagctttac cggaaattcc tgaaccattt aaatatggtg
      781 taggagggat cgataatggt aaaatatata taggactggg aagtctggga aataactggt
      841 atatgataga tacaaaccag agtgaaaaaa aatggactaa gatagcacaa tggccgacag
      901 ttcccagaga gcaggcaaca gctactatta ttgatggtaa gatctatgta tttggaggaa
      961 ttggaaaaga taaaagtgga gtcataacct tacaaaaaga tgtctattcc tatgatattg
     1021 ctaaagataa atgggaaaaa ttgatgaccc gtccccctgt atctcttgcc ggtcatgttt
     1081 cttttatcca taatggtcat gcggtatcta ctggtggtgt aaatgagaat atttttaatg
     1141 gatacttttc agatgtcgag ttatcaaaag ggaattccgc gctgacagag aaagtcaaca
     1201 gggattattt tagtaaacct gttgatgatt attttttaaa taatcatatg atttcttatg
     1261 acccgtcaaa aaaccagtgg aagaacctgg gaacgactcc atttccggga actgctggtt
     1321 catcggtgat ttttgcagaa cagcaaattt atatactggg aggggagaga aaaccgggcc
     1381 ttcgttctgt tagatcctgg acgggggagc tttcgcatga tcgaattaaa tggtctgaac
     1441 ttccaccagt agcttcaccc gagggggtgt caggagcata tgcctctgtt attgatggaa
     1501 atatctttct ggccggtggt gcatatttcc caggggcggc agaaaagtat agcaatggtg
     1561 aatactggtc tcataaagga ctggataaag cctattcaaa agagatatat cagctaataa
     1621 aaaatgactg gaaaaaagtt ggttctttgc cggaaggact tgcttatggt gtaagcctgc
     1681 cctggcaggg ggggatgctc attctgggtg ggg
//
LOCUS       DAZGQL010000273         1698 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.275, whole genome shotgun sequence.
ACCESSION   DAZGQL010000273 DAZGQL010000000
VERSION     DAZGQL010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1698
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.275"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(68..1492)
                     /locus_tag="ACHKIR_005260"
     CDS             complement(68..1492)
                     /locus_tag="ACHKIR_005260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HFO9992841.1"
                     /translation="MFQRSKYDSAYVYALCCVAALAGLMFGYSTAVITGVILPLQQYY
                     QLTSTETGWAVSSIVIGCIIGALVGGKIADKLGRKPALLIIAVIFIVSSLWGAMSESF
                     MVFSISRIVSGFAVGMAGTASTMYMSELAPADIRGKALGIYNISVVSGQVIVFIVNYV
                     IAKGMPSDVLVEEGWKSMLFAQVIPSVTMLVITLFLPESPAWCARNNKGEKRSLKVLA
                     RIYRGVSATDVAAIFDSMRETEARENAAGREFVILKSSPVLRYILLVGCCIAVLQQFT
                     GVNVMNYYAPLVLQNSSTEEVMFQTIFIAVCNVIGSFIGMILFDRYGRIPIMKIGTIG
                     SIIGLLIASYGLYTHDTGYITIFGILFFMLLFAVSWSVGAWVLISEVFPEKIKGFGMG
                     LAVSLMWIANFLISLLFPVINDNTWLQETFGGAFSMWIFVVFNLVCYVFISRYVPETK
                     GIPLTQIEQLAGNKLREIQGKAMM"
BASE COUNT          587 a          437 c          278 g          396 t
ORIGIN      
        1 aaggaaaatc ccggtaagat gctcaacatt accgggcatt actgctgatt gtgacacaat
       61 cagactatta catcatagct ttaccctgaa tttcacgcag tttatttccg gcaagctgct
      121 caatttgtgt tagcgggatc ccctttgttt caggaacata gcgagaaata aaaacataac
      181 aaaccagatt aaagacaaca aaaatccaca tagaaaacgc tccgccaaag gtttcctgca
      241 gccaggtatt atcatttatt accggaaaca acagtgatat tagaaaattc gcaatccaca
      301 ttaaactaac cgccagcccc atgccgaatc ctttaatctt ttcgggaaac acctcagaaa
      361 tcagtaccca tgctcctacg ctccagctga cagcaaacaa cagcataaaa aataaaatcc
      421 caaaaatggt gatatagcca gtatcatggg tatacaaacc atatgatgcg atcaacaaac
      481 caataattga cccgatagtg ccaatcttca tgattggtat tcgtccatag cgatcgaaca
      541 aaatcatccc gataaaacta ccgatcacat tacaaacagc aataaaaatc gtctggaaca
      601 tcacttcctc tgtactgctg ttttgtagca ccagaggtgc gtagtagttc atcacattga
      661 caccagtaaa ctgttgtaaa acagcgatac aacagccaac cagcagaata tagcgcaaca
      721 caggagagct tttcaggata acgaattccc tgcctgcagc attctcccgg gcctccgttt
      781 ccctcatact atcaaaaata gcggctacat cggtagcgct aaccccccgg tagattctgg
      841 caagcacttt cagagaacgt ttttccccct tgttattacg ggcacaccat gccggcgatt
      901 caggcaaaaa taaagttatc actaacattg taacagaagg tatcacctga gcgaataaca
      961 tgctcttcca gccttcttcg accagcacat cagagggcat ccccttagca atcacataat
     1021 taacaataaa aacaatcacc tgaccagaaa ctactgatat attgtaaata cccagagctt
     1081 taccacgaat gtcagctggt gccagttcag acatatacat ggtagatgca gtccccgcca
     1141 tcccaacagc aaaaccactg acgatgcgcg aaatcgagaa aaccataaaa gattcactca
     1201 tcgcccccca cagtgacgag acgataaaaa taactgcaat aatcagaagc gcaggcttac
     1261 gccccaactt atcggcaatt ttccccccga ccagcgcacc aatgatacaa ccaatcacaa
     1321 tgctcgatac tgcccaccca gtttcagttg aagtgagctg gtaatattgc tgtaaaggca
     1381 gtatcacccc cgttatcact gccgtcgaat agccaaacat caatccagcc agtgcagcaa
     1441 cgcagcacaa tgcgtacaca taggctgaat catacttgct tctctgaaac ataccccctc
     1501 cctgcctgtt gaggaaatga catacgaaca tttattccat acttaaacaa atcagctcaa
     1561 atattccaca acaaagatca caaaatataa aaatttcata actgtgaaaa atagtttgta
     1621 gcacaataat catattgaaa atacagaaca ataaaaaact attatttttc atattgataa
     1681 aagttacctg ttgatgag
//
LOCUS       DAZGQL010000274         1665 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.276, whole genome shotgun sequence.
ACCESSION   DAZGQL010000274 DAZGQL010000000
VERSION     DAZGQL010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1665
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.276"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(690..1133)
                     /locus_tag="ACHKIR_005261"
     CDS             complement(690..1133)
                     /locus_tag="ACHKIR_005261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992842.1"
                     /translation="MHHYKFFALIAMLLCGASFASDVYFLCNTEKGTIKLDENKGVLR
                     YTLSKDHKTEFNYESKGSDYSGFKYNHYSRFQTDYFNVSFVNSEYKYTIFSNYEDDRK
                     SSGVSVTSLNSKKESVYYCKSIGIDRLSDLSAKMACDKYSALGCD"
     gene            complement(1120..>1665)
                     /locus_tag="ACHKIR_005262"
     CDS             complement(1120..>1665)
                     /locus_tag="ACHKIR_005262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001026495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramidase family protein"
                     /protein_id="HFO9992843.1"
                     /translation="DYEEAAKELGCEVAAIKAVAKTETGSYGSYFKFEDNDDYVPAIL
                     FERHHFHKYTNGKYDQFEDISNPVAGGYGATSIQYAKLVKAYTLDKKAALKSASWGKF
                     QILASNYATAGYASPEDFVFALSKSEKNQLKAFVSFIKADRVLLRSIRTKDWLSFAQR
                     YNGPRQKGYDLKMERNYNASL"
BASE COUNT          509 a          363 c          273 g          520 t
ORIGIN      
        1 atccaaacag ccccatactt caggttaaaa agaacaataa aaaatgatgc taaaattgca
       61 acattctcta aataaattca attaattatc taaacaaggt gaaactttca gaggaaatga
      121 gcgcggcccc ccctaataat cttaagctgg gtacagggaa gtatcgacca ttacgcagtg
      181 tcgatatcga atcgtacaaa aaacagtctt ggcgtgggat atttacacat aattattagc
      241 tcacaatctc cctatacctg tcgtgttttc tggggattcc tcagggagtg accatagatt
      301 ccgttttcca ggtgcccccc tttttctcca aaaattgaaa aataactctg tagatgccgc
      361 taacaatcaa ttcgagttga tattttaaag cgttctgtgc agaaagaaat aaatttcaaa
      421 aatgaatcag gggagaaaag tgcgcttttt aacctgttca gtgtccagaa cacatgaaac
      481 cgatcgccat tcagtttcat gcatagtcat gcaacgcact gaaaatgatt ctgacgcatt
      541 tttccggttt cccccgagga aaacatccct ttttgcgtca gaatcgcgct cagcgcgttt
      601 tagcggcacg tacaattaca ggattataaa tcgtcaccag cgactggcgt aaaaaagccc
      661 ctctctgcag gcgagattta gatgttttat taatcacacc ccaaagctga atatttatca
      721 caagccattt ttgccgacaa atcactaagg cgatcaatgc caatggactt acaataataa
      781 acagattctt ttttgctatt caggctcgta acactaactc cgctgctttt tctatcatcc
      841 tcataattac taaatatcgt gtacttatac tctgagttta caaacgatac attgaaataa
      901 tcggtttgaa atcgtgagta atggttgtat ttaaaccctg aataatcact acctttcgat
      961 tcataattaa attcagtttt gtggtctttc gataaggtgt accgaagtac acctttgttt
     1021 tcatctagtt tgattgttcc tttttccgtg ttgcataaaa aatacacatc acttgcaaac
     1081 gatgccccac acagaagcat tgcaatcaac gcaaagaact tataatgatg cattgtaatt
     1141 tctctccatt ttcaaatcat atcctttctg ccttggtccg ttatatcttt gagcaaatga
     1201 aagccaatct ttagtccgaa tagagcgcag taatactcta tcagccttaa tgaagctaac
     1261 gaatgctttt aactgatttt tctcagactt acttaatgca aaaacgaaat cctcagggct
     1321 cgcgtagcct gctgtggcgt agttcgacgc taaaatctga aacttacccc aggatgctga
     1381 ttttaaagct gctttcttgt ctaaagtata cgctttaact agctttgcat actgtattga
     1441 tgttgcacca tacccacccg caacagggtt tgatatatct tcaaactgat catacttccc
     1501 atttgtatat ttatgaaaat gatgcctttc aaataatatc gctggaacat aatcatcgtt
     1561 atcttcaaat ttaaaatagg agccataaga accagtttct gtttttgcta cagctttaat
     1621 tgctgcaact tcgcatccta attcttttgc tgcctcttca taatc
//
LOCUS       DAZGQL010000275         1648 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.277, whole genome shotgun sequence.
ACCESSION   DAZGQL010000275 DAZGQL010000000
VERSION     DAZGQL010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.277"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..451
                     /locus_tag="ACHKIR_005263"
     CDS             <1..451
                     /locus_tag="ACHKIR_005263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000264576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFO9992844.1"
                     /translation="RIPGSLPFVTAGEGNEGISAFISNDVVVFPENTITIDMFGSAKY
                     RNYEYGADDHVAVVHTEFFDRNAVIFITASIHKSSCTGKFNYGRNFYAKDADELMVSL
                     PSKDNNPDYSFMETLILAVHKLVIKDVVQYTDKKLVAYRQITSEKVV"
     gene            1287..>1648
                     /locus_tag="ACHKIR_005264"
     CDS             1287..>1648
                     /locus_tag="ACHKIR_005264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFO9992845.1"
                     /translation="MAFKHYDVVRAASPSDLAEKLTQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVTTPVLVKPSDGEGTVISATSDPEYYFVVVLAGQSNGMSYGEGLPLPETY
                     DRPDPRIKQLARRSTVTPG"
BASE COUNT          443 a          362 c          411 g          432 t
ORIGIN      
        1 tcgtattccc ggatccttgc cttttgttac tgcgggtgaa ggcaatgaag gtatttctgc
       61 atttatcagt aatgatgttg ttgtattccc tgaaaatacc attacgatag atatgtttgg
      121 ctccgcaaaa tacagaaatt atgagtatgg tgcagatgac catgtggcag ttgttcatac
      181 agaattcttt gacagaaatg ccgttatttt cataacagca tcaattcata agtcttcttg
      241 taccggaaag ttcaactacg ggagaaattt ctatgcaaaa gatgccgacg agttaatggt
      301 atctttgccg tcaaaagata ataaccctga ttattctttc atggagacgc taattctagc
      361 agtacataaa ctggtcatca aagatgttgt ccagtacact gataaaaagc tggtagcgta
      421 caggcagatt acatctgaga aggtcgttta gtgcaaatcc ccacctgaca gggtggggaa
      481 tggtcaggtt atcgtgccct gatgcagaaa aacagtttac aatcgtaaaa atcatcatat
      541 catgacaaaa gtggttacat tgccacgcag ccgaacccgc cgatgcgcgg gttttttgta
      601 tccggaattc tgtgggttac cctgcccccc ttagaaaggt gcgacagcaa ttaataacaa
      661 aatcttaaaa atcgcatatg gcactattag ttttctaaat attgtatatt ttaagtattg
      721 caggataacc ctgtaacgaa gtttgcgtaa cagcattttg ctctacgagt ttgccagcct
      781 cccccagtgg ctggcttttt ttgtatccgt tcaacaggaa tgttacatac ctcacaatta
      841 agtcagttga atgttgtctg cccggatgag aatttgttag aaaaaaactg catggtgaat
      901 ccccctgtgc ggaggggcga ctggtgaatg gtatgatctc tttgatgatc gtaagcgaga
      961 atacgcgggt ttggtggcac caggccgaac tcaccgggag gcacccggca ccatgcagta
     1021 tacagagatt aggcatatac cagggcccct catagcaggg gcctttttac atgcaaaaaa
     1081 agccgccccg ggaagagcgg ttggcaagaa gaaaagcaat atgaacaatt aattaacgat
     1141 gggaataata ccttagagta atcaccttgc gcaactgtaa gggcatattt catctttgcg
     1201 ggctgttttt ccgggtgact tctgtgtttc cggaggccag cctgtacctt ctctgactca
     1261 gaacattatc ccggccggga ggattcatgg catttaaaca ctatgacgtg gtcagggcgg
     1321 cgtcgccgtc agaccttgcg gaaaaactga ctcaaaaact gaaggagggg tggcagccat
     1381 ttggcagtcc tgtcgccatc acgccttata ccctgatgca ggccattgcg gcggaaggtg
     1441 atgtcaccac acctgtgttg gtgaagccgt cggatggaga aggcacagtt atcagcgcca
     1501 ccagcgaccc ggagtattac tttgttgtgg ttctggcagg gcagtcaaac ggcatgtcgt
     1561 atggtgaagg ccttccgctg ccggagacat atgaccgtcc ggacccgcgt attaagcagc
     1621 tggcgcgccg cagtacggtg acaccggg
//
LOCUS       DAZGQL010000276         1639 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.278, whole genome shotgun sequence.
ACCESSION   DAZGQL010000276 DAZGQL010000000
VERSION     DAZGQL010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.278"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            27..761
                     /gene="nanR"
                     /locus_tag="ACHKIR_005265"
     CDS             27..761
                     /gene="nanR"
                     /locus_tag="ACHKIR_005265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HFO9992846.1"
                     /translation="MSDNKKTVLPGLTTTRKPIVTKKLSEIVAEELERMIRQEEIREG
                     EYLPSERELMAFFGVGRPSVREALSSLRHKGLIRYNKGEKASVCRPSPETIINGLSGI
                     VKDFLSTADGIKDFERLRIFFETNLVRYAAKNATPEDIHRLEKVLSIHHNSLRTPEVY
                     AQYDIEFHSILADIPDNKVLKTIHSAFSEWLIMAKPLEKNKMTYENNKKTHQQHIEIL
                     TAIKEHDCDKAEQLMYNHLYKRIEAS"
     gene            complement(797..>1639)
                     /locus_tag="ACHKIR_005266"
     CDS             complement(797..>1639)
                     /locus_tag="ACHKIR_005266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001305022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO9992847.1"
                     /translation="ISESKSLSGRIYQDPEHNGEFELDRVLTDIYAPQLIIFLPDKPN
                     GTGILIIPGGGYRAVMLDKEGTALAPEFLSQGYTLFVLSYRLPSPAEEENINKTFIDA
                     ISAMLYIRKNADKWHLQRHRIGVLGFSAGGHLASTFVNQICDFKRSSDSKEQEIFAIP
                     DFVGLIYPVISMKDDVTHPDSRKQLLGDNSTSENIIQYSADLNVTSCFPPVFLLHCCD
                     DDLVSIENSLLMYRGLIEKGVSAEMHLYEKGGHGFSVQWATDLPVGGWIKLFMDWMYS
                     HGFK"
BASE COUNT          565 a          343 c          290 g          441 t
ORIGIN      
        1 ttgctctaat ccatataaat caatatatgt ccgacaacaa aaaaaccgtg ttacctgggc
       61 taacaacgac cagaaagccc attgttacaa aaaaactttc agaaatcgtt gccgaagagc
      121 ttgaaagaat gatccgccag gaggagataa gggaaggtga atacctgcct tctgaacgtg
      181 aacttatggc attctttggc gttggaagac catctgtcag agaggcttta tcatctctca
      241 gacataaagg gctaatccgg tataacaagg gagaaaaagc cagtgtctgc cgtccatcac
      301 cagaaaccat cattaatggg ttatcaggca ttgttaaaga ttttttatcc acagctgatg
      361 gcatcaagga ttttgaaaga ctacgcatct tttttgaaac caaccttgta agatatgcag
      421 caaaaaatgc aacgcctgag gatattcaca ggcttgagaa ggtgctcagt atccatcata
      481 actcactgag aacccccgaa gtgtatgctc aatatgatat tgaatttcat agtatacttg
      541 ctgatatacc tgacaataaa gtattaaaaa caatacactc agcattttct gaatggctga
      601 taatggccaa acctctggaa aagaataaaa tgacatatga aaataacaaa aaaacccatc
      661 agcagcatat tgaaatactt acggccataa aagagcatga ttgtgataaa gctgaacagc
      721 taatgtataa tcacctttat aaacgaattg aagcatcata gttactcgac gcttcaccag
      781 gtagtcacca taaatattat ttaaatccat gagaatacat ccagtccata aacagtttta
      841 tccatcctcc aacaggaaga tctgtggccc actgaacact aaagccatgc cctcctttct
      901 catagagatg catttcagca gaaacgcctt tctctattag gccacggtac atgagcagac
      961 tgttttcaat tgacacaaga tcatcatcac aacaatggag taaaaacaca ggaggaaaac
     1021 atgatgtgac attaagatcc gcactatatt gtattatatt ttctgaggta ctattgtccc
     1081 ccagtaattg cttcctggag tctggatgtg tcacatcatc tttcattgat attacaggat
     1141 aaattaaccc gacaaaatcg ggaatagcga aaatctcttg ttctttactg tcagaagaac
     1201 gtttgaaatc acaaatctga ttcacaaacg ttgaggcgag atgcccccca gcagaaaatc
     1261 ctaatacacc aatacgatga cgctgtagat gccatttatc agcattctta cgaatataaa
     1321 gcattgcaga tattgcatca ataaaagttt tgtttatatt ttcttcctca gctggcgatg
     1381 gtaatctgta actaagaaca aataatgtat agccctgaga taaaaactct ggagcaagag
     1441 ctgtaccttc cttatctaac ataacagccc ggtagccccc cccgggaata atcaatattc
     1501 ctgttccatt tggtttatct ggtaaaaaaa taattaactg aggagcataa atatctgtca
     1561 atacacgatc aagttcaaat tccccgttat gctcaggatc ctgataaata cggcctgaaa
     1621 gagatttact ctcagatat
//
LOCUS       DAZGQL010000277         1598 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.280, whole genome shotgun sequence.
ACCESSION   DAZGQL010000277 DAZGQL010000000
VERSION     DAZGQL010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.280"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..512
                     /locus_tag="ACHKIR_005267"
     CDS             <1..512
                     /locus_tag="ACHKIR_005267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO9992848.1"
                     /translation="ASVRATFGSYEAALDDVEAERIIRTSPKFIDDYYWGWTAAEKKA
                     GSIDGVRSNEIDDQRRAYVDGYRDVLPEPHTLSDVVREFIYWDWLYEMRQTAGRETGD
                     KYGFTGEHHESVYDRQFWLENLLGKIKPVTRDEAVEVCRWFLASGKDEYMEDNGSAVI
                     LNLVGECEQ"
     gene            591..1016
                     /locus_tag="ACHKIR_005268"
     CDS             591..1016
                     /locus_tag="ACHKIR_005268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFO9992849.1"
                     /translation="MRDIQQVLSRWGAWVVNNHESVAWSGVAAGFKGVIPSKVKSRPQ
                     CTDDDALIISNCMAQLNVNNSDLHDFLYDYYVFGMTLMGLGRKHERSDCWAGRVLQKA
                     EGVIEGMLIMQGIELEMDRYVERERSGAQVSQFSGHTGN"
     gene            1216..1372
                     /locus_tag="ACHKIR_005269"
                     /pseudo
     CDS             1216..1372
                     /locus_tag="ACHKIR_005269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
BASE COUNT          421 a          331 c          465 g          381 t
ORIGIN      
        1 ccgcatcagt cagggccaca tttggcagct atgaggcagc actcgacgat gtggaggctg
       61 agcggataat tcgcacctcc ccgaaattta tcgacgatta ctactggggc tggactgcgg
      121 cagagaaaaa agccggaagt attgacggtg ttcgctcaaa tgaaattgat gatcagcgtc
      181 gtgcatatgt tgatggctat cgtgatgtac tgccagagcc tcatacattg tcagacgttg
      241 ttcgtgagtt tatttactgg gactggcttt atgagatgcg ccagacagcg gggagagaaa
      301 caggggacaa atacggtttc acaggtgaac atcatgagtc agtatatgat cggcagttct
      361 ggctcgaaaa tttgctggga aaaataaagc ctgtgacgcg tgatgaagcg gttgaagtgt
      421 gtcgctggtt tctggcaagc ggaaaagatg agtacatgga agacaatggt tcagcggtca
      481 ttctcaatct ggttggggag tgtgagcaat gaagccggag atatccagta gacattttcc
      541 ccctgagcat tcaaatcaga aaaagtatgg ttacagcgga gtacagaata atgcgtgata
      601 tccaacaggt tctgtcccga tggggtgcat gggtggtaaa taatcatgag agtgtggcat
      661 ggtccggcgt cgctgccgga tttaagggag tgatcccatc aaaggttaaa tcacggcctc
      721 agtgtaccga tgatgatgcg ctgattatca gcaattgcat ggcgcaactg aacgtcaaca
      781 acagcgattt gcatgatttt ctgtatgatt attatgtgtt cgggatgacg cttatgggac
      841 tgggccgtaa gcatgagcgc tctgattgct gggccgggcg ggtactgcaa aaagctgaag
      901 gtgttattga ggggatgttg attatgcagg gaatagaact ggaaatggac agatacgttg
      961 agcgtgaacg atcaggggca caggtcagtc agttttccgg acatacggga aattgaaagc
     1021 gcggggtttt actgtagaat gactgcgggt gcttgagggt gtctgcctcg ggcatgctgc
     1081 cgtaaggcag acggagaaaa gccccagtta acattacgtg tcctgcaaga cgcttaacat
     1141 taatctgagg cccaatctat gtcacacaac cgtaggttag cctcttacgt gccggaaggc
     1201 aaggagaagc agtctatgaa gcagcaaaag gcgatgttaa ttcgccctga tcgtcatctg
     1261 tttaactgtc atagtgacgg cactggtaac gaggaaagac ctctgcgagg tacggatccg
     1321 aaccggccag acggaggtcg ctgtcttcgt agactacgaa tctgagaagt aagagaccag
     1381 gcgggggagt aatctcccgc cacctctgat gtgtcaggca tcctcaacgc acccgtactt
     1441 tatttgtcat atgctccgca aaatgcaggt tgtcgttgca acccgtgcgg taattttttg
     1501 tgtgcgagtg ctcaaaaaac gttgattttc ataaaaggat gattttatgc tgccagacca
     1561 gcgaccagcg accagcgacc agcgaccagc gaccagcg
//
LOCUS       DAZGQL010000278         1597 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.281, whole genome shotgun sequence.
ACCESSION   DAZGQL010000278 DAZGQL010000000
VERSION     DAZGQL010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.281"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..381
                     /locus_tag="ACHKIR_005270"
     CDS             <1..381
                     /locus_tag="ACHKIR_005270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO9992850.1"
                     /translation="ELQMKINNLIVALSVAPEVTAIAEKIRLLDKELRRASVSLKTLK
                     SKGVNSFSDFYAIDLTSKNGRELCRTLAYKTFEKIIINTDNKTCDIYFMNGIVFKHYP
                     LMKVISAQQAISALKYMVDGEIYF"
     gene            846..1397
                     /gene="ybcL"
                     /locus_tag="ACHKIR_005271"
     CDS             846..1397
                     /gene="ybcL"
                     /locus_tag="ACHKIR_005271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HFO9992851.1"
                     /translation="MKTLIVSTVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGVPEGTKSFAVTVYDPDAPTGSGWWHWTVVNIPATVTYLPVDAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKVWALKTEKIPVDSNSSGA
                     LVGYMLNANKIATAEITPVYEIK"
     gene            1407..>1597
                     /locus_tag="ACHKIR_005272"
     CDS             1407..>1597
                     /locus_tag="ACHKIR_005272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFO9992852.1"
                     /translation="MLARGKTNLKIEEIRMHKHHEIHRVKPLMPALCRIRQGKKVINW
                     ETHTLTVDNNQIILFPCGY"
BASE COUNT          515 a          243 c          344 g          495 t
ORIGIN      
        1 gagcttcaga tgaaaattaa taatttaatc gttgcattgt ctgtcgcgcc tgaagttacc
       61 gctatagcag agaaaataag actattagat aaggaattac gaagggcttc ggtatcattg
      121 aaaactttga agagtaaagg tgtaaattca ttcagtgatt tttatgctat tgacttaacc
      181 agtaaaaatg gacgagagtt atgccgtaca cttgcctata aaacattcga aaaaatcata
      241 attaatacgg ataataaaac ctgtgatatc tattttatga atggcattgt ttttaaacac
      301 tatcctttaa tgaaagtaat atccgcccag caggcgataa gtgctctcaa atatatggtt
      361 gatggtgaga tttatttcta aataatgatc tcggatttta agttatgcta tggtgataaa
      421 gtgcaagaca gaattaatta tctttgacga aacttaatgg gtaattactt tgtttgctcc
      481 cacaagcgag ttttgtacgg ctgtattggg gtagtaaatg agctatacaa tcttaatcat
      541 ttgttaggtg agaactcttg gtcgcagatt caaatactga aaatacgtga caaattatta
      601 tgagcaaaat ggtgtatgtc acgtattttg aatggtaggt taaaaaataa caccgacttt
      661 cgtaggtatt actaataata aagcagagtt tttagatagt atcaatgtgc tttgtgtata
      721 ttgtggcaaa taattgggtt gggggtacaa ttgtgattgc ttttgcatga acattgcgcc
      781 tttatgcata atgagataaa ggaatatcaa ataaaataac gataggtcat aacaaagagg
      841 tttttatgaa aacacttatc gtttcaactg tattggcatt cataacattt tctgcgcagg
      901 ctgcagcatt tcaggtcact agtaatgaaa taaaaacagg agagcaactt acaacgtctc
      961 atgtcttttc tggatttggg tgtgaaggtg gtaatacatc gccctcatta acctggtctg
     1021 gtgttcctga aggtaccaaa agctttgccg taactgtata tgatccagat gcacctacag
     1081 gcagtggttg gtggcattgg actgttgtta atattccagc aacagtaaca tatttgcccg
     1141 ttgatgcagg gagacgtgat ggaacaaaac tgccgactgg tgctgttcaa ggccgaaatg
     1201 attttggcta tgctgggttt ggtggcgcat gtcctcctaa aggagataaa ccacatcatt
     1261 accagtttaa agtatgggct ctaaaaactg aaaagattcc tgtagattct aactccagcg
     1321 gagcgttagt tggttatatg cttaatgcta ataaaatcgc aaccgctgag ataacaccag
     1381 tttatgagat aaagtagggt gagagtatgc tggcaagagg taagactaac ttaaagatcg
     1441 aagaaatacg gatgcataaa catcatgaga ttcatagggt taagcctctt atgccagctt
     1501 tgtgtcgtat ccgtcaggga aagaaagtta tcaattggga gacgcatact ttaactgttg
     1561 ataataatca aataatatta tttccttgtg gttatga
//
LOCUS       DAZGQL010000279         1587 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.282, whole genome shotgun sequence.
ACCESSION   DAZGQL010000279 DAZGQL010000000
VERSION     DAZGQL010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.282"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..1536
                     /locus_tag="ACHKIR_005273"
     CDS             <1..1536
                     /locus_tag="ACHKIR_005273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO9992853.1"
                     /translation="ATGRLASVRTTTSDLDIRIPYATDPAGNRLPDPELHPDSTLTVW
                     PDNRIAEDAHYVYRHDEYGRLTEKTDRIPAGVIRTDDERTHHYHYDSQHRLVFHTRIQ
                     HGEPLVESRYLYDPLGRRMAKRVWRRERDLTGWMSLSRKPEVTWYGWDGDHLTTVQTD
                     TTRIQTVYEPGSFTPLIRIETDNGEREKTQRRSLAEKLQQEGSEDGHGVVFPAELVRM
                     LDRLEGEIRADRVSSESRQWLAQCGLTVERLAAQIEPVYLPERKIHLYHCDHRGLPLA
                     LISTEGATEWRGEYDEWGNLLNEENPHHLHQPYRLPGQQHDEESGLYYNRNRYYDPLQ
                     GRYITQDPIGLEGGWNLYNYPLNPIIRMDPLGLYNLYQLLYDVWHDDSYGTSSIDITG
                     SGDLISLGGHAGLGVAFAKKKGEMLSDICIYATACGHAGIGGGINAAITYSETKSLPT
                     SGVSNSVGVTVGGGVGGHFAYTYVVDVDNPESSTESVGIGAGVDASVMTLACRTWQEC
                     WVN"
BASE COUNT          398 a          366 c          496 g          327 t
ORIGIN      
        1 gccacgggca ggctggcgag cgtgcgcacg accacatcag acctggatat ccgcatcccg
       61 tatgccaccg acccggcggg aaaccgcctg ccggacccgg agctgcaccc ggacagtaca
      121 ctcacagtgt ggccggataa ccgcatcgcg gaggatgcgc actatgtcta ccgccacgat
      181 gaatacggca ggctgacgga gaagacggac cgcatcccgg cgggtgtgat acggacggac
      241 gacgagcgga cccaccacta ccactacgac agccagcacc gcctggtgtt ccacacgcgg
      301 atacagcatg gcgagccact ggtcgagagc cgctacctct acgacccgct gggacggcga
      361 atggcgaaac gggtatggcg gcgggagcgt gacctgacgg ggtggatgtc gctgtcgcgt
      421 aaaccggagg tgacgtggta cggctgggac ggcgaccacc tgacgacggt gcagaccgac
      481 accacgcgta tccagacggt atacgagccg ggaagcttca caccgctcat ccgcatcgaa
      541 acagacaacg gcgagcggga gaaaacgcag cggcggagcc tggcggagaa gctgcagcag
      601 gaaggaagcg aggacggcca cggtgtggtg ttcccggcag aacttgtgag gatgctggac
      661 aggctggagg gggaaatccg ggcagaccgc gtgagcagtg aaagccgcca gtggctggca
      721 cagtgcgggc tgacggtgga gcggctggcg gcacagatag agccggttta tttacctgag
      781 cgaaaaattc atctttatca ctgtgaccac cgggggctgc cgctggcgct catcagtacc
      841 gaaggagcaa cggaatggcg tggggaatat gatgaatggg gcaacctgct gaatgaggag
      901 aacccgcatc acctgcacca gccataccgt ctgccggggc agcagcatga tgaggaatca
      961 ggactgtact ataaccggaa ccggtactat gacccgctac aggggaggta tattacacaa
     1021 gaccccattg ggctggaggg gggatggaac ctgtataatt acccactgaa tccgataata
     1081 aggatggatc ctttgggttt gtataattta tatcaattat tatatgatgt ttggcatgat
     1141 gattcatatg gaacatcatc aattgatatt actggcagtg gagatctaat atcattaggt
     1201 ggtcatgcag gacttggcgt tgcgtttgct aaaaagaaag gtgaaatgtt atctgatatt
     1261 tgtatttatg ctacagcatg tggacatgca ggaattggtg gtgggataaa tgcggctatc
     1321 acatattctg agaccaagtc tttacctaca tcgggagtca gtaattcagt aggtgtaacg
     1381 gttggcggcg gagttggggg gcattttgcg tatacttatg tagtggatgt tgataatcca
     1441 gaatcatcga cagaatctgt tggtatcggt gcaggtgttg acgcttcagt tatgactctg
     1501 gcttgtagaa cgtggcaaga atgctgggtc aattaacagg aggaaatatg aaaaaatata
     1561 tatttttagt tttattttta ttgtgtg
//
LOCUS       DAZGQL010000280         1586 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.283, whole genome shotgun sequence.
ACCESSION   DAZGQL010000280 DAZGQL010000000
VERSION     DAZGQL010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.283"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            242..898
                     /locus_tag="ACHKIR_005274"
     CDS             242..898
                     /locus_tag="ACHKIR_005274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015967619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HFO9992854.1"
                     /translation="MRTLGERLSSARQRAGLTQDALAKKAGITRVAISKAEQGLTKSF
                     NGDTLFKVATALNCTPQWLQNGEEQSVNWENNVKSCPQKDAFHSYPVINWVQAGLFST
                     AGDDYSMYDHDNWRHSVKYAGERGFWLEVHGDSMTSPVGITFPEGMSILVNPDKAVHS
                     GCYVIARKKSTNEATFKKYVTEMGRSFLKPLNPQYPIIEIDDDCEIVGVIVDARWDIF
                     "
     gene            983..1393
                     /locus_tag="ACHKIR_005275"
     CDS             983..1393
                     /locus_tag="ACHKIR_005275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001539125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO9992855.1"
                     /translation="MFQKTTLSCIEDLKNLGSLIALIAKAAPDTTLANDIESCAGMAW
                     DIINKVSHTLSLLIHKQNISDCPHEIRTQRESCGLSTAELATLLGVDEETIIQWENGK
                     AEPLASQVIPLANILDCDPMWLLTGFHSDTTKES"
     gene            1395..1586
                     /locus_tag="ACHKIR_005276"
     CDS             1395..1586
                     /locus_tag="ACHKIR_005276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992856.1"
                     /translation="MKMFKGLTNEPETAFHHIAVLLEAGLIISASGDEECDELSDDIF
                     LLAQQYARSACDAFKEQRT"
BASE COUNT          506 a          349 c          325 g          406 t
ORIGIN      
        1 tcttccggca cacctcgggt tttccacttt ccaacgccct gactacctct aggttttcct
       61 ctcttaggga aacggcgacc aattgcagca ttggttttaa actcagcttt taaaatttcg
      121 taaagagtca tctctcactc actgattgat actttagcgt ccataaatgt acaacaaatg
      181 aatctgaagt atcaagaata tgtgctactt tagtatcgac cgaaaaaaca ggagagataa
      241 aatgagaact ttgggagagc gccttagcag cgccaggcaa cgggcaggat tgacacaaga
      301 tgcattagca aaaaaagccg gtataacccg cgtggctata agcaaagcag agcaaggact
      361 tactaaaagc ttcaacggag atactttatt caaggtagcc acagccctaa actgcactcc
      421 acaatggcta caaaatggag aagaacaatc tgtaaactgg gaaaacaatg ttaagagttg
      481 tcctcaaaaa gacgcatttc attcctatcc cgttattaac tgggtccagg ccggactatt
      541 ctctactgct ggagatgatt acagcatgta tgaccatgat aactggagac attctgtcaa
      601 atatgcaggt gaaagaggtt tttggcttga agtacatggt gattcaatga cctcgcctgt
      661 tggaatcacc tttcctgagg gtatgtctat tctcgtaaac ccagacaaag ctgtacattc
      721 aggctgttac gttatcgcaa gaaaaaaatc aaccaacgaa gcaacattca aaaaatatgt
      781 gactgaaatg ggaagatctt tcctgaaacc actcaatcct cagtatccca tcattgaaat
      841 agatgatgat tgtgaaatcg tcggcgtgat cgtggatgcc cgatgggata ttttctaaga
      901 aagatacttt agcatcaaaa acgcttgccc atgagtgata ctttagttac catactaaaa
      961 aagtaactga ggtatcatta atatgttcca aaaaactacg ctatcatgca ttgaagattt
     1021 aaaaaatctt ggctccctca ttgctcttat cgcaaaagca gcgccagata caactcttgc
     1081 caatgatatc gagtcatgcg ccggaatggc gtgggatata ataaacaaag tatctcacac
     1141 attatctttg ttaattcata aacaaaacat ttctgattgc ccccatgaga tccgcaccca
     1201 acgcgaaagc tgcggtctat ccaccgctga actcgccacg ctgcttggag ttgatgaaga
     1261 aaccatcatc cagtgggaga acggcaaggc tgaacctctg gcaagccagg tgattcctct
     1321 ggcaaatata cttgactgcg atccgatgtg gttacttaca ggtttccatt ctgacactac
     1381 aaaggaatcg taagatgaaa atgttcaaag gcctcaccaa cgaaccggaa acagcttttc
     1441 atcacattgc agtactgctt gaagcggggt taatcatttc ggcttccggt gatgaagaat
     1501 gtgatgaact ttcggatgac atctttttac tggcacaaca atacgccaga agcgcatgcg
     1561 atgcatttaa ggaacaaaga acatga
//
LOCUS       DAZGQL010000281         1520 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.284, whole genome shotgun sequence.
ACCESSION   DAZGQL010000281 DAZGQL010000000
VERSION     DAZGQL010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.284"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..1039)
                     /locus_tag="ACHKIR_005277"
     CDS             complement(<1..1039)
                     /locus_tag="ACHKIR_005277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012884439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFO9992857.1"
                     /translation="MHQYLLFLLGLTPLLAMIILILKVKMPIHYAVLSTLFITLILAT
                     LFWHTPVQDLGSAVVYGAIKGLWPIVIVILAAIYSYNLMNATKSMNILRNVLAAVSDD
                     KRIQVLLISWCFGGFLEAAAGYGTAVAIPIGILIALGFNPLKAAIASLVANTVPTAFG
                     AVGIPVSVLAEQVNLPVTTLSGTIILQLAAFNILLPFVIVAIIGEGLKAIRGVGFMTL
                     MCGVSTLIPQYLVAVYLGAELPAFAGSLVSLIVIVFMAKNRKEKTAPEFLIETSADSA
                     SALSYSRGALLRACSIYILIFLFILLCSPLFPTVKSTVSQIASSLSFSLSNGSNLSMK
                     IEWIATPGVLII"
     gene            complement(1339..>1520)
                     /locus_tag="ACHKIR_005278"
     CDS             complement(1339..>1520)
                     /locus_tag="ACHKIR_005278"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992858.1"
                     /translation="RLPEWPEERLHDLLPFSAYRFAELTDVTVIPPNAGKRVMEVVSS
                     TPMNDTASEVKIPTQ"
BASE COUNT          438 a          319 c          364 g          399 t
ORIGIN      
        1 tgataatcag aacaccgggg gttgcgatcc attcaatctt catcgacaga ttgctgccgt
       61 tactcagaga gaagctgagt gacgaggcaa tctgagacac tgtacttttt accgtcggga
      121 acagtggaga gcaaagaaga atgaacagaa aaatcaggat gtagattgag cacgcacgta
      181 acaacgcccc acgagagtat gaaagtgcgc tggcgctatc tgctgaagtc tcaataagaa
      241 attcaggagc tgttttttct ttacggtttt ttgccataaa gactatcacg atcaggctga
      301 ccagacttcc cgcgaaagca ggaagttctg cgccaagata aacagccacc aggtactgtg
      361 ggattaatgt gctcacaccg cacattaagg tcatgaaccc aacgcctcgt atagccttca
      421 ggccttcccc aataatagcg actataacga agggaagcag aatgttgaac gctgccagct
      481 gaagaataat cgtcccgctg agggttgtta cgggtaaatt tacctgttca gcaagtactg
      541 atacaggtat gccgacagca ccaaacgcgg taggtactgt attagcaacc aatgatgcaa
      601 ttgcggcttt aagagggtta aagcccagtg caatgagtat accaatgggg attgctacag
      661 ctgtaccgta cccggccgcc gcctcaagaa atccaccaaa acaccatgag ataagaagca
      721 cctggatacg tttatcatca ctgacagcgg ccagaacatt tcgcagaatg ttcatgcttt
      781 ttgtcgcatt catcaggtta tagctgtaaa tggcagcaag tatgactatc actatcggcc
      841 acagcccttt gattgcgcca tatacaacag cagaacccag atcctgaact ggtgtgtgcc
      901 agaacaaagt tgcaagaatc agtgtaataa agagtgtgct gagtacggca taatgtatgg
      961 gcattttgac tttcagaatc aaaatgatca ttgccagaag tggggtaagt cccagtaaaa
     1021 aaagcaggta ttggtgcatg tcatttcctc aacgaaaaca aattcagcat cattactgtt
     1081 gttattgccc tgtcaggcga gaatttgtaa ttttcaggca actaccgtcg gtgtgaccaa
     1141 tttacgcttg tctcgcaggg agagaacgag tctcgtccac aaacagggaa gggggttata
     1201 atttgttttt gttatgaaaa atgattgctg aatcgtttta ttgttatttt atttgactaa
     1261 atgttattaa aagaacctgt ttgaatgaac tttgttcaac tatgttgtaa ttgtgttttg
     1321 gaaagcttaa ggctggtttc actgcgtcgg tatttttact tcagaggctg tgtcattcat
     1381 gggagtagag ctgacgacct ccatgactcg tttccctgca ttaggtggta tgaccgtaac
     1441 gtcagttaat tctgcgaatc ggtaggctga gaacggtagt aagtcgtgca agcgctcttc
     1501 cggccattca ggcagacgtg
//
LOCUS       DAZGQL010000282         1513 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.285, whole genome shotgun sequence.
ACCESSION   DAZGQL010000282 DAZGQL010000000
VERSION     DAZGQL010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.285"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>1513
                     /locus_tag="ACHKIR_005279"
     CDS             <1..>1513
                     /locus_tag="ACHKIR_005279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFO9992859.1"
                     /translation="PVKGLQSILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGF
                     ESSGAETALGVEVTKVKPVTRTITSANIDRLRVTFGVQSLVETTSKGDRNPSSVRLLI
                     QLERNGHWVTEKDITINGKTTSQYLTSVILNNLPERPFNIRMVRETADSTTDQLQNRT
                     LWSSYTEIIDVKQCYPNTAIVGLQVDAEQFGGQQLTVNYHIRGRIIQVPSNYDPEKRT
                     YSGIWDGSLKPAYSNNPAWCLWDMLTHPRYGMGKRLGAADVDKWALYAIGQYCDQTVP
                     DGFGGTEPRMTFNAYLSQQRKVWDVLGDFCSAMRCMPVWNGQTLTFVQDRPSDVVWPY
                     TNSDVVVDDNGVGFRYSFSALKDRHTAVEVNYTDPQNGWQTSTELVEDPEAILRYGRN
                     LLKMDAFGCTSRGQAHRAGLWVIKTELLETQTVDFTLGSQGLRHTPGDIIEICDNDYA
                     GTLTGGRILSIDAASRTLTLDREVTLPEAGTSTVNLINGSGKPVRVDITAHPAP"
BASE COUNT          334 a          422 c          491 g          266 t
ORIGIN      
        1 gtcctgtgaa ggggctgcag agtattctgg tgaacaaaac cccgctgacg gacacggacg
       61 gtaatcccgt gatacacggt gtgaccgccg tctggcgcgc cggggagcag gagcagacac
      121 cgccggaagg ctttgagtcc tccggggcag aaaccgcact gggcgtggag gtgacgaagg
      181 taaagccggt gacgcgcacc atcacgtcag cgaacattga ccgtctgcgg gtcaccttcg
      241 gggtacagtc actggtggag accacctcaa agggtgaccg taatccctct tctgtccgcc
      301 tgctgattca gcttgagcgt aacgggcact gggtgacgga gaaggatatc accattaacg
      361 gcaagaccac ctcgcagtac ctgacgtcgg tgattctgaa taatctccct gagcgcccct
      421 ttaacatccg gatggtcagg gagacggcgg acagcaccac ggaccagctg cagaacagaa
      481 cgctgtggtc gtcatacacc gaaatcatcg atgtgaaaca gtgctacccg aacacggcga
      541 ttgtggggct gcaggtggat gcggagcagt ttggtggcca gcagctgacg gtgaactacc
      601 atatccgtgg tcgcatcatt caggtgccgt caaactatga cccggaaaaa cgcacctaca
      661 gcggtatctg ggacgggagt ctgaaaccgg catacagcaa taacccggcc tggtgcctgt
      721 gggacatgct gacccacccg cgctacggga tgggaaaacg cctgggggcg gcggacgtgg
      781 acaagtgggc actgtatgcc atcgggcagt actgtgacca gacggtcccg gatggtttcg
      841 ggggaacaga gccgcggatg actttcaatg cgtacctgtc acagcagcgt aaggtgtggg
      901 atgtcctggg ggatttctgc tcggcgatgc gctgtatgcc ggtatggaac ggccagacgc
      961 tgacgttcgt tcaggaccgt ccgtcggatg tggtgtggcc gtacaccaac agcgatgtgg
     1021 tggtggatga taacggcgtg gggttccgct acagcttcag tgccctgaag gaccggcaca
     1081 cggcggtgga ggtgaattac accgacccgc agaacggctg gcagacctcc acggaactgg
     1141 tggaagaccc tgaagccata ctgcgctacg gacgcaacct gctgaagatg gatgcgttcg
     1201 gctgtaccag ccgcggtcag gcccaccgtg ccggactgtg ggtgataaag acagaactgc
     1261 tggaaacgca gacggtggat ttcacgctcg ggtcacaggg gctgcgtcac acgcccggtg
     1321 acatcattga aatctgtgat aacgactacg ccgggaccct gaccggcgga cgcatcctgt
     1381 ccatcgatgc cgccagccgt accctgacgc tggaccgtga ggtgacactg ccggaagcag
     1441 ggacatcgac ggtgaacctg attaacggca gcggtaagcc ggtgcgcgtg gacatcactg
     1501 cacaccccgc ccc
//
LOCUS       DAZGQL010000283         1489 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.286, whole genome shotgun sequence.
ACCESSION   DAZGQL010000283 DAZGQL010000000
VERSION     DAZGQL010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1489
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.286"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>1489
                     /locus_tag="ACHKIR_005280"
     CDS             <1..>1489
                     /locus_tag="ACHKIR_005280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO9992860.1"
                     /translation="VRTLAPDLDISIPYATDPAGNRLPDPELHPDSTLTVWPDNRIAE
                     DAHYVYRHDEYGRLAEKTDRIPAGVIRTDDERTHHYHYDSQHRLVFHTRIQHGEPLVE
                     SRYLYDPLGRRTGKRVWRRERDLTGWMSLSRKPEVTWYGWDGDRLTTVQTDSTRIQTV
                     YQPGSFTPLIRIETDVTELEKAQRRSLAEKLQQEGSEDGHGVVFPAELVRLLDRLEEE
                     IRTDRVSSESRAWLAQCGLTVEQLEKQVEPEYTPARKVHLYHCDHRGLPLALISEDGN
                     TVWSAEYDEWGNLLNEENPHHVYQSYRLPGQQHDEESGLYYNRNRYYNPLLGRYITQD
                     PIGLAGGWSLYAYPLNPVNGIDPLGLSPADVALMRKKEQLNHQRAWDILSDTYDDMKR
                     LNLGGTDQFFHCMAFCRVSKLNDAGVSRSAKGLGYEKEIRDYGLNMFGMYGRKVKLSH
                     SEMIEDNKKDLAVNEHGLTCPLTQDCSNRCIDYINPEHKKTIKALQ"
BASE COUNT          397 a          344 c          464 g          284 t
ORIGIN      
        1 gtgcgcaccc tcgcaccgga cctggatatc agcatcccgt atgccaccga cccggcggga
       61 aaccggctgc cggacccgga gctgcacccg gacagtacac tcacagtgtg gccggataac
      121 cgcatcgcgg aggatgcgca ctatgtctac cgccacgatg aatacggcag gctggcggag
      181 aagacggacc gcatcccggc gggtgtgata cggacggacg acgagcggac ccaccactac
      241 cactacgaca gccagcaccg tctggtgttc cacacgcgga tacagcatgg cgagccactg
      301 gtcgagagcc gctacctcta cgacccgctg ggccgccgga cgggaaaacg ggtatggcgg
      361 cgggagcgtg acctgacggg gtggatgtcg ctgtcgcgta aaccggaggt gacgtggtac
      421 gggtgggacg gcgacaggct gacgacggtg cagactgaca gcacgcgtat ccagacggtg
      481 taccagccgg gaagcttcac accgctcatc cgcatcgaaa cagacgtgac cgaactggag
      541 aaagcgcagc ggcggagcct ggcggagaag ctccagcagg aagggagcga ggacggacac
      601 ggcgtggtgt tcccggctga actggtgcgg ctgctggaca ggctggagga agaaatccgg
      661 acagaccgcg tgagcagtga aagccgggcg tggcttgcgc agtgcgggct gacggtggag
      721 caactggaaa aacaggtgga gccggaatat acaccggcgc gaaaagttca tctttaccac
      781 tgcgaccacc ggggcctgcc gctggcgctt atcagcgaag acggcaatac ggtgtggagc
      841 gcggagtatg atgaatgggg aaacctgctt aatgaggaga acccgcatca tgtgtatcag
      901 tcataccgtc tgccggggca gcagcatgat gaggagtcgg ggctgtacta caaccggaac
      961 cggtactaca atccgttgct gggacgatat attactcagg acccgattgg gctggcgggg
     1021 ggatggagtc tgtatgcgta tccgctgaat ccggtgaatg gtattgatcc attaggatta
     1081 agtcctgcag atgtagcgtt aatgcgaaaa aaagaacaat taaaccatca aagagcatgg
     1141 gatatattat ctgatactta tgatgatatg aagagattaa atttaggtgg gactgatcaa
     1201 tttttccatt gtatggcatt ttgtcgagtg tctaaattaa atgacgctgg tgttagccga
     1261 tcagcgaaag ggctgggtta tgaaaaagag attagagatt acgggttaaa tatgttcggt
     1321 atgtacggca gaaaagtaaa gttatcccat tctgagatga ttgaagataa taaaaaagac
     1381 ttggctgtaa atgaacatgg gttgacatgt ccattaacac aagattgctc aaatagatgt
     1441 attgattata ttaatccaga gcataaaaaa acgataaagg ctttacaag
//
LOCUS       DAZGQL010000284         1480 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.287, whole genome shotgun sequence.
ACCESSION   DAZGQL010000284 DAZGQL010000000
VERSION     DAZGQL010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1480
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.287"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>1480
                     /locus_tag="ACHKIR_005281"
     CDS             <1..>1480
                     /locus_tag="ACHKIR_005281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPD5 domain-containing protein"
                     /protein_id="HFO9992861.1"
                     /translation="PAPENAAGRQGETLEGEIVRRGLPSPDAQNATAPVREGLPAPDI
                     ARNVRMPQPESLPRTVRDSLPELAQQAEVRRQAGGNRDIPQPETIAPESETTVFTDRE
                     STVRGGEVRGKKIEDFGEEIKGAAKHRYAQLAETLGKTLEDRDYATQPLSKLFPKPDY
                     AKLANEGADADTLAMIALYRSDIPAKTKHNTAGWGESVKKVRHSVSEMLNGTVSAKRL
                     AEWMEGRMPSRYADTWQLLRTLPPSQMDRASAYRVVSGVYQAAGGKRYDPPQKLYSLR
                     NKDNKGSNLFFSESRDELLAKAKVWFAEQEEKSQAKGDEKTAPSPDDKIRFDVYRNTR
                     SGDIFIAYGKNKMRVRGGFKSASDARKYIDSHRDELVRHVKDMREISREEQRNATNRD
                     RTGPERRKGDVSPEQFSDAFGFRGVQFGNYVEGPRRQADLNRAYDSLHDLAEVLNVPT
                     KALSLNGRLGLAFGARGKGKAAAHYEPGEVAINLTKGNGPGAL"
BASE COUNT          393 a          355 c          443 g          289 t
ORIGIN      
        1 ctccggcacc ggaaaacgcc gctggtcgcc agggggaaac actcgagggt gaaatagttc
       61 gtcgtggtct gccgtcaccg gatgcgcaga acgcgacagc accggtacgt gaagggctac
      121 cggctcctga cattgcgcgt aatgttcgta tgcctcagcc tgaatcactt ccccgcactg
      181 tacgggactc actgcctgag ctcgcacagc aggcagaagt acgccgacag gccggaggaa
      241 atcgtgacat cccgcagcct gagacaatcg cacctgaatc tgaaacaact gtctttactg
      301 acagggaatc taccgtgcgc ggaggtgaag tcaggggcaa aaaaattgaa gactttggcg
      361 aggaaattaa aggggcagcc aaacaccgtt atgcacagct tgctgaaaca ctgggtaaaa
      421 cgctggaaga cagggattat gccacgcagc cgctgagcaa actgttcccg aaaccggact
      481 acgcaaaact ggcgaacgaa ggtgccgatg ctgacactct ggcaatgata gcgctgtatc
      541 gtagcgatat tccggcgaag acgaaacaca atacggcagg ctggggggag agcgtaaaaa
      601 aagtacgaca cagtgtatcg gaaatgctga acggaacggt cagcgcgaaa cgcctcgcag
      661 aatggatgga aggcagaatg ccctcccgtt acgcggatac ctggcaactg ttacgcactc
      721 tgccaccctc acagatggac agggcttctg cttatcgggt ggtatcgggt gtgtatcagg
      781 cggcaggagg gaagcgttac gatccgccac agaaacttta ttcactgcgc aataaggaca
      841 ataaggggag taacctcttt ttctcggaaa gcagggatga attactggca aaggcgaaag
      901 tctggtttgc agagcaggag gaaaaatcac aggcgaaagg tgatgaaaaa acagcaccgt
      961 caccggatga caaaatccgc tttgacgttt accggaatac ccgcagtggc gatattttta
     1021 tcgcttacgg taaaaacaaa atgcgggtga gaggtggctt taagtcagcc agtgatgcgc
     1081 gtaagtacat tgattcacat cgtgatgagc ttgttcgtca tgtgaaggat atgcgggaga
     1141 tttcgcgtga ggagcagcgt aacgccacca accgcgaccg taccggacca gaacgccgta
     1201 aaggggatgt ttcaccggag cagttcagtg atgcgtttgg tttccgtggt gtgcagtttg
     1261 gtaactacgt ggaaggtccg cgtcgtcagg ctgatttgaa ccgggcttat gactcgctgc
     1321 atgaccttgc ggaagtactg aatgtaccga caaaagcgct ttccctgaac ggtcgtcttg
     1381 gcctggcatt tggtgcccgt ggtaagggta aggcggcggc acactatgag ccgggtgagg
     1441 tggcaatcaa cctgacaaaa ggtaacggac cgggtgcgct
//
LOCUS       DAZGQL010000285         1479 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.288, whole genome shotgun sequence.
ACCESSION   DAZGQL010000285 DAZGQL010000000
VERSION     DAZGQL010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1479
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.288"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..782
                     /locus_tag="ACHKIR_005282"
     CDS             <1..782
                     /locus_tag="ACHKIR_005282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3396 domain-containing protein"
                     /protein_id="HFO9992862.1"
                     /translation="DGKQRYEAWLMWLCDTFSVESGYAGLSFALPYARERMFPCEFAL
                     AQRFSGVMVDSLGTLEGGGAVIGLKGACWYTILGTPWLEKLGGAERVKHRLAKTPEIS
                     LLPYNNGVVLKAGELPPPLGEMKTEGLSPLLVKVNQVIKPVRFNESRSLHFYSEYEGL
                     QFDKESTMKWYRRFDEASALLDKEEMGKSCAPERIKCWTDEIAPHAGQWAAMVNGTTE
                     YIQTREGQKMPPFEDKYGKQHRACWKLLKRDDNGSVYTLPE"
     gene            808..1187
                     /locus_tag="ACHKIR_005283"
                     /pseudo
     CDS             808..1187
                     /locus_tag="ACHKIR_005283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004160259.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2345 domain-containing protein"
     gene            1184..>1479
                     /locus_tag="ACHKIR_005284"
     CDS             1184..>1479
                     /locus_tag="ACHKIR_005284"
                     /inference="COORDINATES: protein motif:HMM:NF024894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4123 domain-containing protein"
                     /protein_id="HFO9992863.1"
                     /translation="MNSVSFSLAAQHLICSTKVTLFALVDGLQYERYFGESLSVQQPA
                     AVPLFDTWPDSRIAFAGPWVMEMNSIMDFRERLCELEAALPSVSWMISSSSLTEL"
BASE COUNT          356 a          326 c          449 g          348 t
ORIGIN      
        1 ctgatggaaa gcagcgttat gaagcctggc tgatgtggct gtgtgacacc ttttcggtgg
       61 aaagtggtta tgcagggttg tcgtttgcac ttccttatgc ccgtgagcga atgttcccct
      121 gtgaattcgc cctggcgcag cgtttttccg gtgtgatggt ggattcactg ggaacgctgg
      181 aaggcggtgg ggcagtcatt ggtctgaaag gtgcctgctg gtacaccatt ctcggtacgc
      241 cgtggctgga aaaactgggg ggggcagaga gagtaaaaca tcgtttagct aaaacgcctg
      301 aaatcagcct gctaccctat aacaatggtg tcgtacttaa agccggtgaa ctccccccgc
      361 cactgggaga gatgaaaaca gaaggtttat cgccgctgct ggtgaaggtg aaccaggtca
      421 ttaagccagt gcggtttaat gaatcaagat ctcttcattt ctactctgaa tatgaaggac
      481 ttcaattcga caaagaatcc accatgaagt ggtacaggcg ctttgacgaa gccagcgccc
      541 tgctcgataa agaagaaatg gggaaatctt gcgcccccga acgcattaaa tgctggaccg
      601 atgaaatagc gccccatgcc ggacaatggg ccgcgatggt taatggcacc accgagtaca
      661 tccagacccg cgaagggcag aaaatgcccc cgtttgagga taagtacggt aagcagcaca
      721 gggcttgctg gaaactactg aaacgagatg acaatggtag cgtttataca ctacctgagt
      781 aaggtcgtgt gctgaatgac cgcgcaggcc ggagagaggc tggggctttt tgcccggacc
      841 ggacaactga gcgtcaagtc cggggagggg ccggtagagg ttcaggcgaa aaacggcagc
      901 atgcggttgt ttgctgagaa gaagctgaca ctgagctcag cgtgtgatat ctcttttgcc
      961 gggaaaaaac gtatcacgct gattggcggg ggagttacct gcgacttgaa gccgggaagg
     1021 tcgagtatgg tacgacggca aagtatctgc gcaaagtgaa gcggacaatg gcggcggggg
     1081 ctgcatcaat accagtcagg tccattgtgg ggggaggtat ttgcctgagc tgtctgatga
     1141 aggcggcaat gaatggcgac acctttgttg taaggggtgg gtcatgaaca gcgtcagttt
     1201 tagccttgcg gcacaacacc tgatttgttc aacaaaagtg acattatttg ctctggtgga
     1261 tggtctgcaa tatgagcggt atttcggtga gagtctgtct gttcaacaac ctgccgccgt
     1321 gcccctgttc gatacctggc ctgattcgcg tattgcattt gcggggccat gggttatgga
     1381 gatgaacagc ataatggatt tcagagaaag gctgtgtgag cttgaagctg cgcttccgtc
     1441 agtttcctgg atgatatctt cttcctcgct tactgaact
//
LOCUS       DAZGQL010000286         1478 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.289, whole genome shotgun sequence.
ACCESSION   DAZGQL010000286 DAZGQL010000000
VERSION     DAZGQL010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1478
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.289"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            62..1468
                     /gene="gndA"
                     /locus_tag="ACHKIR_005285"
     CDS             62..1468
                     /gene="gndA"
                     /locus_tag="ACHKIR_005285"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFO9992864.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIV
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLTNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
BASE COUNT          374 a          354 c          376 g          374 t
ORIGIN      
        1 agtaaacttt ttaagttatt ctcataccgc acatgcggta aaaccctgac aggagtaaac
       61 aatgtcaaag caacagatcg gcgtcgtcgg tatggcagtg atggggcgca acctcgcgct
      121 caacatcgaa agtcgtggtt ataccgtctc tattttcaac cgttcccgtg aaaagacgga
      181 agaagtgatt gtcgaaaatc caggcaagaa actggttcct tactatacgg tgaaagagtt
      241 tgttgaatct ctggaaacgc ctcgtcgcat cctgttaatg gtgaaagcag gtgcaggcac
      301 ggatgctgct attgattccc tcaagccata cctcgataaa ggtgacatca tcattgatgg
      361 tggtaatacc ttcttccagg acaccattcg tcgtaaccgt gagctttctg ccgaaggctt
      421 taattttatc ggtaccggtg tttccggtgg tgaagaaggt gcgctgaaag gtccttccat
      481 tatgcctggt gggcagaaag aagcctatga actggttgca ccgatcctga ccaaaatcgc
      541 cgcagtggct gaagacggtg agccatgcgt tacctatatt ggtgccgatg gtgcaggtca
      601 ctatgtgaag atggttcaca acggtattga atacggcgat atgcagctga ttgctgaagc
      661 ctattctctg cttaaaggtg gcctgaatct caccaacgaa gaactggcgc agacgtttac
      721 cgagtggaat aacggtgaac taagcagcta cctgatcgac atcaccaaag atatcttcac
      781 caaaaaagat gaagacggta actacctggt tgatgtgatt ctggatgaag cggctaacaa
      841 aggtaccggt aaatggacca gccagagtgc gctggatctc ggcgaaccgc tgtcgctgat
      901 taccgagtct gtgtttgcac gttatatctc ttctctgaaa gatcagcgcg ttgccgcatc
      961 taaagttctc tctggtccgc aagctcagtc agcaggcgac aaggctgagt tcatcgaaaa
     1021 agttcgtcgt gcgctgtatc tgggcaaaat cgtttcttac gctcagggct tctctcagct
     1081 acgcgcagcg tctgaagagt acaactggga tctgaactat ggcgaaatcg cgaagatttt
     1141 ccgtgctggc tgcattatcc gtgcgcagtt cctgcagaaa atcaccgatg cttatgccga
     1201 aaatccgcag atcgctaacc tgctgctggc tccttacttc aagcaaattg ccgatgacta
     1261 ccagcaggcg ctgcgcgatg tcgttgctta tgcagtacag aacggtatcc cggttccgac
     1321 cttcgccgct gcggttgcct attatgacag ctaccgtgcc gctgttctgc ctgcgaacct
     1381 gatccaggca cagcgtgact atttcggtgc gcatacttat aagcgcattg ataaagaagg
     1441 tgtgttccat accgaatggc tggattaatc tgatttaa
//
LOCUS       DAZGQL010000287         1474 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.290, whole genome shotgun sequence.
ACCESSION   DAZGQL010000287 DAZGQL010000000
VERSION     DAZGQL010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1474
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.290"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..201
                     /locus_tag="ACHKIR_005286"
     CDS             <1..201
                     /locus_tag="ACHKIR_005286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HFO9992865.1"
                     /translation="KTRYLWNVLLNGDFELLNQLVESNEKELKDCNLLGKRDKRRAKI
                     LASKNYILWRMVRLVSRKKNKR"
     gene            247..>1474
                     /locus_tag="ACHKIR_005287"
     CDS             247..>1474
                     /locus_tag="ACHKIR_005287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012602581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFO9992866.1"
                     /translation="MSFCWNEINSGVKSLILILCMLSLMTLSLWDDVATKFLHAAGII
                     SALYFLATPKKTITNNPTLLIFISLCLLGIVNIIWYSHYKVSGSVYTNAYRGPMETGK
                     IALCSAFIFLVLFAKDEMRTKIKFGKLILFASLATQLLFFAHAMWQHFYLNVDRVALS
                     ASHATTAGYIILFPSLLASILILKSDFRHKTTLYTINFMLSLCAVIVTETRAAILVFP
                     FFALLLIVMDSYINKRINYKLYCFIAIALLAGVFSFKDTLLTRMNDLNRDLVNYSHDN
                     TRTSVGARLAMYEVGLKTYSPIGQSLEKRAEKIHELEEKEPRLSGALPFVDSHLHNDL
                     IDTLSTRGIPGVALTILAFSAIFIYALRTAKEPYILILLFSLLVVGLSDVILFSKPVP
                     TAVFVTIILLCAYFKAQ"
BASE COUNT          458 a          243 c          255 g          518 t
ORIGIN      
        1 aaaactcgtt atttatggaa tgttctactt aatggcgatt ttgaattatt aaatcaatta
       61 gttgaaagca atgaaaaaga attgaaagat tgtaatcttc ttggcaagag agataagagg
      121 cgagcaaaaa tattagcatc aaaaaactat attctctgga ggatggtaag actggtaagt
      181 agaaaaaaga ataaacgtta ggtcttgcat taaataaccc atcctcgtag cataggttga
      241 aattatatgt cgttttgttg gaatgaaatt aactctggtg tcaagtcttt aattctcata
      301 ttatgtatgt tgtctttaat gactttgtct ttgtgggatg atgttgcaac aaagtttctt
      361 catgcagctg gaattatatc tgcattatat tttctggcga caccaaaaaa aacaataact
      421 aataatccta ctttgttaat tttcatctca ttatgtcttt tgggtatcgt taatatcatc
      481 tggtattcac attataaagt ttcaggctcg gtttatacca atgcatatcg tggcccaatg
      541 gaaactggaa aaattgcctt gtgtagtgct tttattttct tagttctttt tgctaaagat
      601 gagatgagaa ccaaaataaa atttgggaaa ctaattctgt tcgcatccct ggcaacgcag
      661 ttactttttt ttgcgcatgc catgtggcaa catttctatt taaacgtcga ccgtgttgca
      721 ttatcagcgt cccacgctac aacagcaggc tacatcatcc ttttcccttc tttactggca
      781 tcaattctca ttttaaaatc cgactttaga cataaaacaa cattatatac aattaacttc
      841 atgcttagct tatgtgctgt tatagtaact gaaacgcgtg cagccatatt agtgtttcca
      901 ttctttgcgt tactattaat cgtaatggat agttatatta ataagcgaat taattataag
      961 ttatattgtt ttattgcgat tgcattatta gcaggtgtat tttcttttaa agatacatta
     1021 cttacgagaa tgaatgactt aaatagggat ttagttaatt attcgcatga taacaccaga
     1081 acttcagtcg gtgcccgtct ggcaatgtat gaagttggtt taaaaacata ttctccaata
     1141 ggacaatcac tggaaaaacg tgcggaaaaa atacatgaac tagaagagaa agagcctaga
     1201 ttaagtggcg ctttaccctt tgtagattct catttgcata acgatctcat agatacgtta
     1261 tcaacgcgtg gtattcctgg agtagcatta acaattttag ccttttcagc aatattcata
     1321 tatgccttaa gaactgctaa agaaccttat attttaatct tgcttttttc actactggta
     1381 gtaggcctaa gtgatgtaat actcttttct aaaccggttc cgactgctgt gtttgtcacc
     1441 ataatattgc tttgtgctta ttttaaagca caat
//
LOCUS       DAZGQL010000288         1474 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.291, whole genome shotgun sequence.
ACCESSION   DAZGQL010000288 DAZGQL010000000
VERSION     DAZGQL010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1474
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.291"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            25..>1474
                     /locus_tag="ACHKIR_005288"
     CDS             25..>1474
                     /locus_tag="ACHKIR_005288"
                     /inference="COORDINATES: protein motif:HMM:NF014452.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S6 family peptidase"
                     /protein_id="HFO9992867.1"
                     /translation="MTLSALPGKASTVSAEIPYQTFRDFAENKGVFTPGVTGIEIKDN
                     NGNAVGTLDVPMIDFSSVSRRGSLTLLSQGYGVSAKHGDLGDVNNASFGYDKNNYTVV
                     KNNKHSGLDFSLHRFSKLITEAAPADINIFGQLSDSSQYTAFYRAGAGTQYIKERSGK
                     QTHIPGTFLTGGTVGTPWYSGNNLISSSPGDTYNKSQGPLASYGQMGDSGSPLFAYDS
                     LSEKWSLAGVTLHNYGVNGYRNNWLLLPEDYIRNIIVADFDPIISFNKNSKEHMSWTY
                     DAAKGVGRIQQDDQQFVMHGNLNGNLNAGKNLYFTGENGIIDLKDNVNQGAGYLQFAD
                     DYTVTTSNDSSWSGGGIIVNYGTTVKWGINGVFGDDLHKVGDGTLIINGTGKNEGGLK
                     IGAGTVILEQKAKNNDSTAFSSINISGGNSRVKLSGDNQIIPDNVSWGFRGGYLDING
                     KDTEFSRLQAVDYGAAIINSSIDKSLLTLNLSP"
BASE COUNT          456 a          262 c          344 g          412 t
ORIGIN      
        1 atgacggttt ccctggtatt atcagtgact ctttctgctt taccgggtaa agcatcaacg
       61 gtcagtgcag aaataccata tcagactttt cgtgactttg ctgaaaataa aggtgtgttt
      121 acccccggag tgacaggaat tgaaataaaa gataataatg gaaatgcagt tggtacgctt
      181 gatgtgccaa tgattgactt cagcagcgtg tcacggcggg gctcactgac cctgttgtcg
      241 cagggatatg gtgtatcagc aaaacacgga gaccttggtg atgtaaacaa tgccagtttt
      301 ggctatgata aaaataatta tactgtagta aagaacaaca aacattcggg ccttgatttt
      361 tctctgcatc ggttcagcaa actcatcaca gaggctgcac ctgctgatat aaatatattc
      421 ggacaactta gtgattcgtc acagtatact gctttttaca gagcaggcgc tggcacacag
      481 tatattaaag aacgctcagg taaacagact catatcccgg gaacattttt aactggtgga
      541 acagtaggca ctccatggta ttcaggaaat aatttaatat caagctctcc aggtgacacc
      601 tataataaat cacagggacc attagccagt tatgggcaga tgggtgacag cgggtcgcca
      661 ttatttgctt atgattctct cagtgaaaaa tggtctctgg ctggagtcac cctgcataac
      721 tatggggtga acggttacag aaataactgg ttgttattac ctgaggatta tatcagaaat
      781 attattgtag ccgatttcga tcccattatt tcatttaata aaaacagtaa agaacatatg
      841 tcatggacat atgatgcagc caaaggcgtg ggccgtattc agcaggatga ccaacagttt
      901 gtgatgcatg gtaacctgaa tggtaatctg aatgcaggta aaaatcttta ttttacaggc
      961 gaaaatggca ttattgattt aaaagataat gttaatcagg gtgccggata tcttcagttt
     1021 gctgatgatt atacagtaac aacgagcaat gacagttcat ggtccggagg aggcattatt
     1081 gtcaattatg gtacaactgt aaaatggggt ataaacgggg ttttcggaga tgaccttcat
     1141 aaagttgggg acggaacgct tattattaat ggaaccggga aaaatgaagg gggactaaag
     1201 attggggctg gtactgtaat ccttgaacaa aaagcgaaaa acaacgattc aacggctttt
     1261 tcttccatta acatcagtgg gggaaactcg cgcgttaaac tttcaggaga taatcagatt
     1321 atacctgata atgtttcctg gggattccgg gggggatatt tagatataaa cggaaaagac
     1381 actgagttct cccggttaca ggctgttgat tatggggctg caattattaa cagcagcata
     1441 gacaagtcat tattgacttt aaatctgtca ccat
//
LOCUS       DAZGQL010000289         1474 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.292, whole genome shotgun sequence.
ACCESSION   DAZGQL010000289 DAZGQL010000000
VERSION     DAZGQL010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1474
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.292"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(394..768)
                     /locus_tag="ACHKIR_005289"
     CDS             complement(394..768)
                     /locus_tag="ACHKIR_005289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992868.1"
                     /translation="MDTHDKIPPAYSYSPQPVPEAWKMRFSANSAWFFTDDLTVVDAW
                     LKAHSDGVEVVPLFCGSDSDGKRDTAFEEVCAALARMLHMHRLLLEKTRYQEHVTDPE
                     CLRELHEAPIQALNILVKHKLS"
BASE COUNT          413 a          345 c          324 g          392 t
ORIGIN      
        1 ctacgaaaat ttcgtaagtc ctaccccgca caaggattaa ataatcacta caagcgattg
       61 attaaactta attatcgcga atcgcgagaa ttagccccgc agaaacaagg aaagaagaat
      121 tttacgaaaa tttcgtaaaa tccccccaca tctcccacgg ttgcccgtta agtgcaagcg
      181 cacaggcacg acctcattaa ccagttacca gcgctcacgc tcaaatatcc acaggaacgg
      241 ggctaaatcg cattctggcg caatgttcac tgtcagggcg agtattcacc agaaaacgtc
      301 atacaggctt acagtcggct taaaattcga taggcggagg accggatggg aatcgggaga
      361 cctggacaaa aagtgcaggc ctccatgaca cggtcaggac agtttgtgtt taaccagaat
      421 atttagcgcc tgtattgggg cttcgtggag ctcccgcaga cattcagggt cagtaacatg
      481 ttcctggtat cttgttttct caagtaacaa gcggtgcata tgcagcatac gggcaagagc
      541 cgcgcacacc tcctcaaatg cggtatcccg cttaccgtca ctgtcagagc cacaaaacag
      601 cggtacaacc tccacgccgt cgctgtgggc ctttagccag gcatcaacca cggttaaatc
      661 atcggtgaag aaccacgcac tgttagcgct gaatcgcatt ttccaggctt ccggtactgg
      721 ttgcggggag tatgaatatg ctggtggtat cttgtcgtga gtatccatag gctatcgttc
      781 cgtcattctg ttattcacca cagcgctaaa aacgccatga aggaccagaa taccgattgc
      841 ctcgtttgct gtatgaggtg cttaccacac attgggtaca cagatgaatc aggcggaagt
      901 gtgattaccg gaaaaaaccc ctttcagttt gacgaaaaaa aaaccgcagt gtgataacac
      961 tacggcacgc caccaaagaa acataacaag gaatcagcaa tgagtaatac ctgtaagcct
     1021 tcggggtgat tgtaacacat gagcaatctt ttactggaag ctgtttttca ctggatcacg
     1081 tatcgttatg gggattccca tatcggagag catccaccag cttcatagcg gataaccgga
     1141 aaaattccgg taaatatttg cctgagtaag tcatcaagcg cctgctaaat gtgtatagaa
     1201 atgtgtatag gattgatttt agaatatgtg ggaatggctt tattgctggt ggcatggctt
     1261 gttgttctgt tcccttcgcc gctccaattt atcttcacat catctacctg ccatttttgt
     1321 tgtatctgat tttctatatt atcttaattc aagcgcttca gacttttcgc aatcgccgta
     1381 atacttcccc atttttttgt aaataaaaag cgtttacgca atgcaatcgt catgtatctt
     1441 aaatagtctt atttaagatg tttagaactc tttg
//
LOCUS       DAZGQL010000290         1458 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.294, whole genome shotgun sequence.
ACCESSION   DAZGQL010000290 DAZGQL010000000
VERSION     DAZGQL010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1458
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.294"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(161..>1458)
                     /locus_tag="ACHKIR_005290"
     CDS             complement(161..>1458)
                     /locus_tag="ACHKIR_005290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFO9992869.1"
                     /translation="FSEAKIADTSQVETSARYLGTGRQWSVSGPHIKPGKDFWFYVRS
                     VNLVGKSAFVEASGRASNDAEGYLDFFRGEIGKTHLAQGMWELIDNSQLDDEMAEMKT
                     TITETRNEITQTVSKTLEDQSATIQQIQRVQTDTNNDLAALYMLKVQKTKNGIPYVAG
                     IGAGIEDADGQPLSNILLQADRIAMINPENGNTTPLFVAQGNQLFMNDVFLKRLFAVS
                     ITSSGEPPTFSLTPDGKLTARNADISGNINANSGTLNNVVIAENCTIKGTLRAENIIG
                     DVVKTHNVSLPALSAAGDHRHATERTVTVIDDQSFDRNIVIPPVFYSGIVYQGSDGDG
                     ATSICTLTVSVNDRVVFSKSSSIGTGRTSGGYPVQKDTMYPGSSTPFSYFMRMASGGG
                     DVRIKFRIEVIYYGTAPGFMTTGIQITTMKATASGFMES"
BASE COUNT          310 a          429 c          334 g          385 t
ORIGIN      
        1 atttgctgaa gatcgaaagt caggcctcgg gttaaacatg aatgtttgtc cggggccttc
       61 gtctttccgg ccttcaggtg ttccctccgg ccatcagcca aaggcacccg cgcatactgt
      121 acggtttttg tctccttccg gcaatcacga tcgtccggga ttaactttcc ataaatcctg
      181 aggctgtcgc cttcatcgtt gtgatctgta tccctgtggt cataaaaccc ggtgcagttc
      241 cataatagat gacctcaatc cggaacttga tcctgacatc tcctccccct gacgccatcc
      301 tcatgaaata gctgaacgga gtggatgacc cgggatacat ggtgtctttc tgaacggggt
      361 atccacctga cgtacgcccg gtcccgatgg atgagctttt gctgaacact acccgatcat
      421 tcacacttac cgtcagcgta cagattgaag ttgcaccatc accatcactt ccctggtaga
      481 caataccgga ataaaaaacc ggcgggataa caatgttccg gtcaaacgac tgatcatcaa
      541 tgacagtaac cgttcgttct gtcgcgtgtc tgtgatcccc tgctgcactg agagcaggaa
      601 gactgacatt gtgcgttttc accacatcgc caatgatatt ttccgctctc agagtacctt
      661 tgatggtaca gttctccgct atcacgacat tattgagcgt gcccgagttc gcattaatat
      721 tcccgctgat atccgcatta cgggctgtca gtttcccgtc cggcgtcagg gaaaacgtcg
      781 gaggctcacc tgatgaggta atactcaccg caaacagtcg tttcaggaac acatcgttca
      841 tgaacagctg attcccctgc gccacaaaca gcggcgtggt gttgccgttc tccgggttaa
      901 tcatcgcgat acggtccgcc tgcagcagta tattgctcag gggctggcca tcagcatcct
      961 caatccccgc acctataccg gcaacatacg gaatgccgtt tttcgttttc tgcaccttca
     1021 gcatgtacag cgcagccagg tcgttatttg tgtctgtctg cacccgctgt atctgctgta
     1081 tggtggcact ctggtcttcc agggttttac tgaccgtctg cgtgatttca ttgcgggttt
     1141 ctgtgatggt ggtcttcatc tccgccatct catcatcaag ctggctgtta tcaatcagct
     1201 cccacatccc ctgtgccaga tgtgtcttcc cgatttctcc tctgaaaaag tccagatacc
     1261 cttccgcatc attgctggcc cggccactgg cttccacaaa cgcagatttc cccaccaggt
     1321 tgacgctgcg cacataaaac cagaaatcct tccccggctt aatgtgcggg ccggagacac
     1381 tccactggcg gcctgtcccc agataacggg cagaggtttc cacctgagat gtgtctgcga
     1441 tttttgcctc cgaaaacc
//
LOCUS       DAZGQL010000291         1453 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.295, whole genome shotgun sequence.
ACCESSION   DAZGQL010000291 DAZGQL010000000
VERSION     DAZGQL010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1453
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.295"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..1001)
                     /locus_tag="ACHKIR_005291"
     CDS             complement(<1..1001)
                     /locus_tag="ACHKIR_005291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HFO9992870.1"
                     /translation="MRSFLTSKAGSVVQQFSGQSRYRVHVHECSHFLDVLRYSAVESL
                     SQPWRYDVVVTCSSADIACDALLLKPASFTFQTPVSDGTPALPLRTVFGVVESFRRIS
                     ASNDETRYALTIVPRIALLNYTKGSEIYLNQSVTEVVEQVLRKHGLEGQDFEFRLSRE
                     YPPRELITRWRETDLEFIRRLLAEVGIYWRYEMDSRLEQDIVIFQDSQQQYESGITLP
                     LRNQAGMSDSGQESIWDIQTTCNVVSGSVVTRDYNYREALKPQDSAESIFSKEGITTG
                     ETYHYAEPFLTAGDAESPETGAYFARLRHERILNAQYLVSGHSSSPYLTPGQVLETDG
                     "
     gene            complement(986..>1453)
                     /locus_tag="ACHKIR_005292"
     CDS             complement(986..>1453)
                     /locus_tag="ACHKIR_005292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000431644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HFO9992871.1"
                     /translation="SDLLMQLLDEQPEATEASLHELLRPILRDHFQPALLARFRTVIY
                     RPLTQSAMRTIVEMKLAQVCERLHCHYGLSTSVDERVYDALTSACLLPDTGARNVESL
                     LNQQLLPVLSRQLLSHMAAKQKPQALTLAWSDEDGIVLVFDAQAEGESSCAVS"
BASE COUNT          322 a          432 c          391 g          308 t
ORIGIN      
        1 ccgtccgttt caagtacctg acccggcgtc agataagggc tggaggaatg cccgctgaca
       61 agatactgcg cattcagaat acgttcgtga cgcagacggg caaagtatgc cccggtctcc
      121 gggctttcag cgtcacctgc cgtcaggaag ggttcagcat aatggtacgt ttctccggtg
      181 gtgatccctt ccttactgaa aatactttcg gcactgtcct gaggtttcag cgcttcacgg
      241 tagttgtagt cccgggtgac cacacttccg ctcaccacgt tacaggtggt ctgaatatcc
      301 cagatgctct cctgtccgct gtcactcatc ccggcctgat tgcgcaatgg cagcgtgatg
      361 ccagattcat actgctgctg gctgtcctgg aaaatcacta tgtcctgctc aagacggctg
      421 tccatttcat aacgccagta aatccccacc tccgccagca ggcgccggat aaactcaagg
      481 tcagtttccc gccaccgggt gatgagctcc cggggtggat actcccggga gagccggaac
      541 tcaaaatcct gcccttccag accatgttta cgcaatacct gctcaaccac ttcagtgact
      601 gactgattga ggtagatttc gctccctttc gtatagttca gaagggcaat acggggaacg
      661 atcgtcagtg cgtaacgggt ctcatcattc gaggcagaaa tgcgacgaaa cgactcaacc
      721 acgccgaata ccgttctgag cggcagggcc ggtgttccgt cagacaccgg ggtctggaaa
      781 gtaaatgacg ccggcttcag cagtaacgcg tcacaggcaa tatccgcaga ggagcaggtg
      841 acaaccacgt catagcgcca gggctggctg agggactcaa ccgcgctgta acgcaacaca
      901 tccagaaaat ggctgcattc atgcacatga acccggtacc gtgattgccc tgaaaactgc
      961 tgtacaacgc tgccagcctt gctggtcagg aaactgcgca tgacgactct ccctctgcct
     1021 gtgcatcaaa taccagtaca atgccatcct cgtcactcca tgccagagtc agcgcctgcg
     1081 gtttctgctt cgcggccata tggctcagca actgccggct cagtaccggc agcagctgct
     1141 ggttcagcag actctccaca ttccgggcac ccgtatccgg cagcaggcag gctgatgtca
     1201 gggcatcgta cacacgttca tcaaccgatg tgctcagccc ataatggcag tgcaggcgtt
     1261 cgcagacctg tgcaagcttc atctccacaa tggtgcgcat ggcagactgc gtcagcggac
     1321 ggtaaatcac ggtccggaaa cgggcgagca gtgcaggctg gaagtggtcg cgcagtatcg
     1381 ggcgcagcag ttcgtgcagg ctggcctcag tggcttccgg ttgttcgtcc agcaactgca
     1441 tcagaaggtc act
//
LOCUS       DAZGQL010000292         1451 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.296, whole genome shotgun sequence.
ACCESSION   DAZGQL010000292 DAZGQL010000000
VERSION     DAZGQL010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1451
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.296"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..935)
                     /locus_tag="ACHKIR_005293"
     CDS             complement(<1..935)
                     /locus_tag="ACHKIR_005293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001178612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO9992872.1"
                     /translation="MQNDDSVTKITWEQLLEEYFFSKNLRPDTEWSYCKVVRVFREFI
                     GDILPGEVTVRDVQRYRRYVLKEQKRREVTWNNKVAHLRAIMGFGIKMKLLPQTENPL
                     VEASVASTEKKKKTLSDNQMLRVYLVVQRFAEAEEQNMVNSRRRNAIYPAWYWLTVLD
                     VLRYTGMRFNQLKHIRVRDVCPDESIIDLKLEGSKTHREWGVPLVAPLKVSMEKLLER
                     ARQCGAGDDDYLFDVCRFSEPISADRYQYCPVRAHQTVRSFFRRLSRECGFPVSPHRF
                     RHTLATRLMKYPERNLHLVKSILGHRSLTTTMGYV"
     gene            complement(1003..1407)
                     /locus_tag="ACHKIR_005294"
     CDS             complement(1003..1407)
                     /locus_tag="ACHKIR_005294"
                     /inference="COORDINATES: protein motif:HMM:NF019154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HFO9992873.1"
                     /translation="MEKTAEFSLTEDSRVESEFIRWLKEGLREGSLSVNSVDSNVHVV
                     SGYVFLCAPGIFYRFLKESGSGQDRRYVQSLFERAGIHKVRSGERFFQARLYSDAEHS
                     GRYKMVSGYLVKGRFLYGNITVPGDSPLIVFG"
BASE COUNT          376 a          420 c          278 g          377 t
ORIGIN      
        1 tcaacatacc ccatggtcgt tgtaagactc ctgtgtccca gtatggattt gaccagatgt
       61 aaattacgtt caggatattt catcagtctg gttgccagcg tatgtctgaa acgatgtggt
      121 gaaacaggaa aaccacattc tcgcgaaagt cgccggaaaa atgaacgtac ggtctgatgc
      181 gcccgaacgg gacagtactg atatctgtcg gcagagatcg gctcactgaa acgacagacg
      241 tcaaaaagat agtcatcatc acctgccccg cactgacggg cccgctccag cagtttttcc
      301 atcgatacct tcaggggagc caccagagga acgccccatt ccctgtgagt tttacttccc
      361 tccagcttaa gatcaattat gctttcatcc ggacagacat ccctcacacg gatatgcttc
      421 agttgattaa agcgcattcc ggtataacgc agaacatcca gaactgtcag ccagtaccag
      481 gcagggtata tagcattgcg tcgacgacta ttcaccatgt tctgctcttc cgcttctgca
      541 aacctctgca caaccaggta aaccctgagc atctgattgt cactcagtgt ctttttcttt
      601 ttttcagtac tcgcaactga tgcttccacc aacgggtttt ctgtctgagg cagcagtttc
      661 atcttgatcc cgaatcccat aattgcccgc aagtgggcca ccttgttatt ccaggtcacc
      721 tccctgcgct tctgctcttt caggacataa cgccggtatc gctgaacatc acggacagtc
      781 acctcccccg gcaaaatatc accgataaat tccctgaata ccctgacaac tttgcagtag
      841 ctccactctg tatccggccg aagattcttt gaaaaaaagt actcttccag cagttgttcc
      901 caggtgattt ttgtgacgga atcgtcattc tgcattatat attccccttg tttactgtgc
      961 tcccgcactc tccggtcaaa ttctgcacat catcgccttt actcacccga aaacaataag
     1021 cgggctgtct ccgggaaccg taatattccc gtacagaaac ctgcccttaa ccagatatcc
     1081 cgaaaccatt ttgtaacgtc cggaatgttc tgcgtcacta taaagacggg cctgaaaaaa
     1141 acgctctcct gaacgtacct tatggatacc tgcccgttca aagagcgact gaacataacg
     1201 acgatcctgc ccggaaccac tctccttcag aaaacgataa aaaatccccg gggcacacag
     1261 aaaaacatat ccggaaacaa catgaacatt tgaatcaacg ctgttaaccg aaagtgaacc
     1321 ctccctcagc ccctctttta accatctgat aaattcactc tctaccctgc tatcctctgt
     1381 cagggaaaac tctgccgttt tttccactgt ttccgggata cggtttccct gattttctgt
     1441 tacacatccg c
//
LOCUS       DAZGQL010000293         1445 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.297, whole genome shotgun sequence.
ACCESSION   DAZGQL010000293 DAZGQL010000000
VERSION     DAZGQL010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1445
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.297"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>1445
                     /locus_tag="ACHKIR_005295"
     CDS             <1..>1445
                     /locus_tag="ACHKIR_005295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001340207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFO9992874.1"
                     /translation="CQTSDQRPATSDQRPATSDQRPATSDRKSRKSVKSVEPDIADRA
                     NNWLKSYGLDYKLEQAPLNNEIDNALDAYFSKNGGTGGNRPDVKLLVQDKYGKQYPVL
                     IEYKGYKDRLIRLGSNGIIENKDARKEWNFKNINGYAVNGAVHYANALLQFTNYPDII
                     AIGMTGWRDDGTGELHHEIGVWYVSKNNLGAGQKVGEFTDLSFLADKNVDGFLNKIKL
                     LNLPPEELEKIKASKEEEIDTRLSRLNNDIYQNEKGLGESDRVYLVVATVIATLGIPG
                     KLAPLDKKELTSSTEEDLRDGDIIFRKIRNFLRLKAVPETKREMILRSLQNTLWTENI
                     NKPVNGESQLKRVFVKVVDDLGEYYKIGLTTDFTGKLFNEMYRWLGFTQDKLNDVVLT
                     PPYVATLLARLARVNKDSYVWDFATGSAGLLVAAMNEMLIDARENIHSPNELQLKEAQ
                     IKAEQLLGLEVLSSIYMLAILNMILMGDGSS"
BASE COUNT          443 a          298 c          350 g          354 t
ORIGIN      
        1 tgccagacca gcgaccagcg accagcgacc agcgaccagc gaccagcgac cagcgaccag
       61 cgaccagcga ccagcgaccg taaatcaaga aagtctgtaa aatctgttga accagacatt
      121 gccgaccggg ctaacaactg gctcaaatct tatggacttg attacaaact ggagcaggca
      181 ccacttaaca acgagatcga taacgcactg gatgcttatt tttcgaaaaa tggcggcaca
      241 ggcggtaatc gtcccgacgt aaaactcctt gttcaggaca aatacggcaa gcagtatccc
      301 gttctcatcg agtataaagg ctacaaggac aggcttatca gacttggcag caacggtatt
      361 attgagaaca aagatgccag aaaagaatgg aattttaaaa atattaatgg atacgcggtg
      421 aatggcgcag tccattatgc caacgcactt cttcagttca ccaattaccc tgatattatt
      481 gctattggta tgaccggctg gcgcgatgat ggtacaggcg aactgcatca tgaaatcggc
      541 gtgtggtacg tatcaaagaa taatttagga gccgggcaga aggttggtga attcaccgac
      601 ttatcgttcc ttgctgacaa aaatgttgat ggatttctca acaagataaa gctgcttaat
      661 cttcctcccg aagaactgga aaaaatcaag gccagcaaag aggaagagat agacacgcga
      721 ctttcccgtc tgaataacga tatttaccag aatgaaaagg ggcttggtga aagcgatcgt
      781 gtttaccttg ttgtggctac cgttattgca acgttgggga ttccgggaaa gttagcaccg
      841 ctggataaaa aagaactgac ctcttcaacg gaggaggact tgcgtgatgg tgatattatt
      901 ttcaggaaaa taaggaattt tttaaggctg aaagctgttc ctgaaacaaa aagagaaatg
      961 attttgcgct cgttgcaaaa tacgctgtgg actgaaaaca ttaacaaacc agtcaacggt
     1021 gaaagccagt taaagcgcgt gtttgttaaa gttgttgatg atcttgggga gtattacaaa
     1081 atcggactga ccactgattt tacgggcaaa ctgtttaatg agatgtatcg ctggcttgga
     1141 tttacgcagg acaaactcaa tgacgtcgtt ctgacaccac cttatgttgc cacattactg
     1201 gcacgactcg ccagagtaaa caaagattcc tatgtgtggg attttgctac aggttctgcg
     1261 ggattgcttg tggctgcaat gaatgaaatg ctcattgacg ccagagaaaa tattcactca
     1321 ccgaatgaac tacaacttaa ggaagcgcaa atcaaggctg aacagcttct tggccttgag
     1381 gtgttatcca gtatttatat gctggccatc ctgaatatga ttttgatggg ggatggtagc
     1441 tcgaa
//
LOCUS       DAZGQL010000294         1444 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.298, whole genome shotgun sequence.
ACCESSION   DAZGQL010000294 DAZGQL010000000
VERSION     DAZGQL010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1444
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.298"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..388
                     /locus_tag="ACHKIR_005296"
     CDS             <1..388
                     /locus_tag="ACHKIR_005296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFO9992875.1"
                     /translation="LGIYWQLSSLPEGVTLSEEGNDMLMSFFLQTDATNLNAMYHKKQ
                     NAKLDTFGVFDNGWHTLAFEFAGNNSIQVTPVLDEKRGTPFTLVKSPASGAADKLQLT
                     GISKAATYTLLIDSVKVEVNNADAAA"
     gene            539..721
                     /locus_tag="ACHKIR_005297"
     CDS             539..721
                     /locus_tag="ACHKIR_005297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFO9992876.1"
                     /translation="MTFLNQLMLYFCTAVCVLYLLSGGYRAVRDFWRRQIDKRAAEKI
                     SASQSAGSKPEEPLIP"
     gene            759..1004
                     /locus_tag="ACHKIR_005298"
     CDS             759..1004
                     /locus_tag="ACHKIR_005298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFO9992877.1"
                     /translation="MSEIKSLVTAEAVKEVLRSEEVRSALKQKLRQNLESRLDAEVDA
                     ILDELLGAPAVPEPEGIAGDGSASDGGEPTPDSDMMM"
     gene            1081..1296
                     /locus_tag="ACHKIR_005299"
     CDS             1081..1296
                     /locus_tag="ACHKIR_005299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO9992878.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIKEDRRKAARGE"
     gene            1300..>1444
                     /locus_tag="ACHKIR_005300"
     CDS             1300..>1444
                     /locus_tag="ACHKIR_005300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1327 domain-containing protein"
                     /protein_id="HFO9992879.1"
                     /translation="MNKQYELVVKGINNYPDKITVTVALEIAGQPSLLSPYVAISLDR
                     TEGA"
BASE COUNT          379 a          319 c          427 g          319 t
ORIGIN      
        1 cctgggtatt tactggcagt tgtcgtccct gccggaggga gtgacgctgt cagaggaagg
       61 caacgacatg ctgatgtcct tcttcctgca gacggatgcg acgaacctga acgcgatgta
      121 ccacaagaag cagaatgcga agctggatac gttcggggtc tttgataacg gatggcacac
      181 actggctttt gagtttgccg gaaacaacag cattcaggtg acgccggtac tggatgagaa
      241 acggggtaca ccgttcacac tggtgaaatc tccggcatca ggggcggcgg acaaactgca
      301 actgacaggc atatcaaagg cggcgacata tacgctgctg attgacagtg tgaaggtgga
      361 agtgaacaac gcggatgccg cggcatgata aaaaaggccg ccagcaatga aggaaaggga
      421 tgaactttgt ccactacatg aatgctggcg gctggtaaaa cgaccatgat gacaccgaag
      481 tctgcggtgt ttttctacaa aaataagtaa atcatgtcaa ctatcacggt gagaaaccat
      541 gacatttctg aaccagttaa tgctgtactt ctgtacggcg gtctgtgtgc tgtatcttct
      601 ttcgggtggg tacagggctg tgcgcgattt ctggcgcagg cagattgata aaagggccgc
      661 agagaaaatc agcgccagtc agtcagccgg aagcaaaccc gaagagccgc tcattccgta
      721 gtcactttct taacaacacc tttcaacgag aaaatcctat gtcagaaata aaatcgctgg
      781 tcactgctga agcagtgaag gaagtcctgc gctctgaaga agtcagaagc gcactgaaac
      841 agaaactccg ccagaacctt gagtcgcgtc ttgatgcaga agtggatgcc attctggatg
      901 aactgctggg cgcaccggca gttcctgagc cggaaggcat tgcgggtgac gggagtgctt
      961 cagatggcgg tgaacccaca cccgacagcg acatgatgat gtaagcatgc gcaagggacc
     1021 atcggtgtgt gtcggtggtc tttatattgt tgtgagcttc cggattgcgg gagacggggt
     1081 atgtaccaga tggaaaaaat cacaacaggt gtgtcataca ccacgtcagc ggtgggaacg
     1141 ggctactggt tcctgcagtt gctggacagg gtttccccgt ctcagtgggc ggcaataggc
     1201 gtgctgggga gtctgctgtt tgggctgctg acatacctga ctaacctgta tttcaagatt
     1261 aaagaagacc gtcgtaaggc ggcgcgggga gagtagacga tgaataaaca atacgaactg
     1321 gttgttaaag ggataaataa ttacccggat aagattactg ttactgtggc actggaaatt
     1381 gccgggcagc cgtcgttgtt gtcgccatat gtggcgatta gtcttgaccg tactgaaggt
     1441 gcca
//
LOCUS       DAZGQL010000295         1439 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.299, whole genome shotgun sequence.
ACCESSION   DAZGQL010000295 DAZGQL010000000
VERSION     DAZGQL010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1439
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.299"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..1245
                     /locus_tag="ACHKIR_005301"
     CDS             <1..1245
                     /locus_tag="ACHKIR_005301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000514889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFO9992880.1"
                     /translation="FEFWFSEAKIADTSQVETSARYLGTGSQWSVSGPHIKPGKDFWF
                     YVRSVNLVGKSAFVEASGRASNDAEGYLDFFRGEIGKTHLAQGMWELIDNSQLDDEMA
                     EMKTTITETRNEITQTVSKTLEDQSATIQQIQRVQADTNNDLAALYMLKVQKTKNGIP
                     YVAGIGAGIEDADGQPLSNILLQADRIAMINPENGNTTPLFVAQGNQLFMNDVFLKRL
                     FAVSITSSGNPPTFSLTPEGRLTARNADISGHISANSGTLNNVVIAENCTINGTLKAE
                     NIIGDLVKCAGVAFPVDGSYLANGTRTLTVYDDHSFDRQIIIPPIIYVGSKQESRTSN
                     DIWTECFLHVDQNGRRIYSGRSVTEPGIFSGIIDMPAGGGHITLSFTVSSRRQNGSFG
                     SSRISNLQAIVVKKNSAGISIR"
BASE COUNT          372 a          340 c          430 g          297 t
ORIGIN      
        1 tttgagttct ggttttcgga ggcaaaaatc gcagacacat ctcaggtgga aacctctgcc
       61 cgttatctgg ggacaggcag ccagtggagt gtctccggcc cgcacattaa gccggggaag
      121 gatttctggt tttatgtgcg cagcgtcaat ctggtgggga aatctgcgtt tgtggaagcc
      181 agtggccggg ccagcaatga tgcagaaggg tatctggact ttttcagagg agaaatcggg
      241 aagacacatc tggcacaggg gatgtgggag ctgattgata acagccagct tgatgatgag
      301 atggcggaga tgaagaccac catcaccgaa acccgcaatg aaatcacgca gacggtcagt
      361 aaaaccctgg aagaccagag tgccaccata cagcagatac agcgggtgca ggcagacaca
      421 aataacgacc tggctgcgct gtacatgctg aaggtgcaga aaacaaaaaa cggcattccg
      481 tatgttgccg gtattggtgc ggggattgag gatgctgatg gccagccctt gagcaatata
      541 ctgctgcagg cggaccgtat cgcgatgatt aacccggaga acggcaacac cacgccgctg
      601 tttgtggcgc aggggaatca gctgttcatg aacgacgtgt tcctgaagcg actgtttgca
      661 gtgagcatca cctcgtccgg caatcccccg acgttctccc tgacgccgga gggcaggctg
      721 acggcccgca atgctgacat cagcggacat atcagtgcga actcgggcac gctgaataat
      781 gtcgtgatag cggagaactg tacgataaat ggcacgctga aagcggagaa cattattggt
      841 gatcttgtga aatgtgcagg ggtggctttt ccggtggatg gtagttacct tgcgaacggt
      901 acacgaacgc tgacggtgta tgacgatcac agctttgacc ggcagattat aatcccgccg
      961 ataatctatg tcgggtcaaa acaggaatcc cgcaccagta atgacatctg gacagagtgc
     1021 ttcctgcatg ttgatcagaa cggacgccgg atttattccg gcaggtcagt gacagagccg
     1081 ggaattttca gcgggatcat cgatatgcca gccggaggtg gtcatatcac cctgagtttt
     1141 accgtaagtt cacggcgtca gaacgggagt tttggcagtt cacgtatcag taaccttcag
     1201 gcgatagtgg tgaagaaaaa cagcgcgggg atcagtatcc gctgaacatc gcgccccggg
     1261 attgccggaa ggatacaaaa accgtacatt atgtgcgggt gcctttggct gatggccgga
     1321 gggaacacct gaaggccgga aagacgaagg ccccggacaa acattcatgt ttaacccgag
     1381 gcctgacgtt cttccttcag caagaagaag gttagcgcct ctccgtaaaa ggagcaagc
//
LOCUS       DAZGQL010000296         1425 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.300, whole genome shotgun sequence.
ACCESSION   DAZGQL010000296 DAZGQL010000000
VERSION     DAZGQL010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1425
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.300"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..938
                     /locus_tag="ACHKIR_005302"
     CDS             <1..938
                     /locus_tag="ACHKIR_005302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001590684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HFO9992881.1"
                     /translation="INKVLLSKNKNVRQPSDTLLLEKKNSKTVTIIGQVINDFSIICT
                     AKNYISTIDFYIELIDKLLCDDDVFIVFKSHPWERQKNNVRSALTLDKINEHIKSLSL
                     DKSSRVLLTEHFNLEGLIKQSDYIVTLCSQSAIEAAFWGMKPIQLGNAFYGQKGFTHD
                     YDSIDEFYADLQKNKLNKYLTVDEYDNLECFLVKFLEKSLISVHKSGILSLEKKLKLP
                     SYISMVQPVIKEVKYKDVDRISKNNSEKVSNADIVHHKENNMNLDNVKSPLIIKEYKN
                     EKAILKKIKKLKRNPKAFLIDSKHRSLNLLARFLF"
     gene            1010..1131
                     /locus_tag="ACHKIR_005303"
                     /pseudo
     CDS             1010..1131
                     /locus_tag="ACHKIR_005303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1159..1425
                     /locus_tag="ACHKIR_005304"
                     /pseudo
     CDS             1159..1425
                     /locus_tag="ACHKIR_005304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537322.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YadA C-terminal domain-containing protein"
BASE COUNT          478 a          230 c          286 g          431 t
ORIGIN      
        1 caattaataa ggttcttttg tctaagaata aaaatgttcg gcaaccctct gacacattgc
       61 tactcgagaa aaaaaacagt aaaacagtta ctattattgg acaagttatt aatgatttct
      121 ctattatatg tacagcaaaa aattacattt ccacaataga tttttatatt gaactgattg
      181 ataagttatt atgtgatgat gatgttttta ttgtgtttaa atcccatcca tgggaaaggc
      241 aaaaaaataa tgtgcgttcc gctttaacat tagacaaaat aaatgaacat attaaatctc
      301 tttcactgga taaatcatcc agagtacttt tgacagagca ttttaatttg gagggtttga
      361 ttaaacaatc ggattacata gtgactttat gctctcagtc tgcaatagaa gccgcatttt
      421 ggggaatgaa acctattcag cttgggaatg cattttatgg acaaaaagga tttactcatg
      481 attatgatag tattgatgaa ttttatgcgg atttacagaa aaacaaactc aataaatatc
      541 ttactgttga tgagtatgat aatttagagt gttttcttgt aaagttctta gagaaaagtt
      601 taatttcagt ccataagtca gggattttaa gtttggagaa aaaacttaaa ttaccatctt
      661 atatatctat ggttcaacca gttataaaag aagttaaata taaagatgta gacagaattt
      721 caaaaaataa ctcagaaaaa gtttccaatg ctgatattgt acatcataaa gaaaataata
      781 tgaatcttga taatgttaaa tctccattaa taataaaaga atataaaaat gaaaaggcaa
      841 tattaaaaaa aataaaaaaa ctcaaacgta atcctaaagc ttttcttatt gactctaagc
      901 acaggtcttt gaatttgtta gccagatttc tgttctaatt ttggatagca gaatatgcaa
      961 tgttattttt cgaatcctcc ataactgata tatgtgctgt tagtggagta caagaataag
     1021 ctcgcgttag cagtctgtca gtactgcgac agcgttgtct gggagggctt tacccggtca
     1081 aaacagcatt attgtgcacc ccatgtgacg gatgaactgt gtgctcaggg ggcattttac
     1141 tgtaaaaacc gtgctgcggt cagccgtaac cgtcaggtgg gtgaacgcaa cagtcgcgcc
     1201 attgcttttc aggcacgtac cctgcaggcg catgagcgtc gtatgaacag ccagcagcgt
     1261 cagattaatg agaatcatga ggaaatgaag cgggcagcag cccagtcagc tgcgctggcc
     1321 ggtcttttcc agccatacag tgtgggtaaa tttaacgcca cagcggctgt gggcggacag
     1381 tgacaggcag gctgttgcgg tgggcatggg atatcgcttt aacga
//
LOCUS       DAZGQL010000297         1416 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.301, whole genome shotgun sequence.
ACCESSION   DAZGQL010000297 DAZGQL010000000
VERSION     DAZGQL010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1416
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.301"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(77..424)
                     /gene="tnpB"
                     /locus_tag="ACHKIR_005305"
     CDS             complement(77..424)
                     /gene="tnpB"
                     /locus_tag="ACHKIR_005305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO9992882.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNGFNGLAAKVQTALKDDPMSGHVF
                     IFRGRSGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
     gene            complement(421..801)
                     /locus_tag="ACHKIR_005306"
     CDS             complement(421..801)
                     /locus_tag="ACHKIR_005306"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO9992883.1"
                     /translation="MQKNVTPGRRKGCPNYPPEFKQQLVAASCEPGISISKLALENGI
                     NANLLFKWRQQWREGKLLLPSSESPQLLPVTLDAAAEQPESLAEDAETLSISCEVTFR
                     HGTLRFNGNVSEKLLTLLIQELKR"
     gene            1095..1415
                     /locus_tag="ACHKIR_005307"
                     /pseudo
     CDS             1095..1415
                     /locus_tag="ACHKIR_005307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997724.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          336 a          376 c          375 g          329 t
ORIGIN      
        1 gatgtcgtca gaagagatgt cgttcataag cccgtatttt accgggctta ttctgtgaca
       61 accaggataa agagatttac agcatggtca gggaggtcag cagccgctta ggctgtcgcc
      121 agtcgatacc ttccagcagc atcgccagct gcgcctgcgt aaggaacact ttgccatcac
      181 gggctgacgg ccaggcgaag cgcccacgct ccagccgttt ggtcaggagg cacagcccgt
      241 caccggtgga ccacagcagt ttaacctgac tgccgctgcg gccccggaaa atgaaaacat
      301 ggccggacat gggatcgtct ttcagcgccg tctgtacttt cgcagccagg ccgttgaagc
      361 catttctcat atcggtgata ccggcaacca gccaaatttt ggtcccggaa ggtaacggga
      421 tcatcgcttc agttcctgta tcagcagagt caggagcttt tcgctgacat tgccattgaa
      481 gcggagcgtc ccgtgccgga acgttacctc acagctgata ctgagggttt ccgcgtcctc
      541 tgcgagcgat tctggctgtt cggcagctgc atcgagagtc acaggaagta gctgggggct
      601 ctctgaagaa ggtaatagca gctttccctc gcgccattgt tgtcgccatt tgaacaacag
      661 attggcgtta atgccatttt caagagcaag ttttgagatg gatatcccgg gttcacagga
      721 ggcagcaacg agctgctgtt taaattcggg aggataatta gggcagcctt ttcgcctgcc
      781 gggagtcaca tttttctgca tatctgacac tttggttccc actacttatt tggtggacac
      841 cactttgtct aattcgtcag attctgacca gacggttcag gctgtgcgct tacgaacaaa
      901 cacggcgcgt tcgtcttcat gaaacaccgg ccgcctggtg ccacacacgc cgggaatgct
      961 acagcaaaac gaccggtcgt cgctgcggca gccctgacag tctcagcagg ctgattctga
     1021 gcagtattca gcccatagaa tgagataacc cgtatgaatg atgacagacg tctacggtac
     1081 ccgatgtatg gaacattttc tttctggcta acggcatgcc cgacacatgg ttttacaccg
     1141 atgacgcctc cgtcacaaaa gagtgggggt gggaatattg tgacaatgtg gaagtgttcc
     1201 ctctgtgcta tatgcatgac ggtcccgttt tccgggggcg ctatattgat gagctcagcg
     1261 ccgcacggag cagaatgctc catatgcacc acatactgcc ggaatgaacc cggtacagag
     1321 aaaattttca tgacgcagaa tgtatccgtg aaattcacga agcgcccctg caggctcccg
     1381 gcattctgca aaaatacaaa cgcacaaaat cataag
//
LOCUS       DAZGQL010000298         1408 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.302, whole genome shotgun sequence.
ACCESSION   DAZGQL010000298 DAZGQL010000000
VERSION     DAZGQL010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1408
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.302"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..877
                     /locus_tag="ACHKIR_005308"
     CDS             <1..877
                     /locus_tag="ACHKIR_005308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFO9992884.1"
                     /translation="LGQKCRVVLVGDCYQQIYRFRGANNALSHPALKNADRLWLTQSF
                     RFGPAVARMANLLLQREGETREVKGCGGDDEVLLKCHAREHLQGHYTVLSRTVAGVIG
                     TALMAAMKGQKVYWVGGIEGYRTGELEDLYWFQVDMPERMHSDRLRRDYRNFEEYKYI
                     AKSTKDVEMNQSLRLLELCFPLPKKLELLRQYTVTNEQDADITVSTAHRSKGLEWPVV
                     MLDEDFQDITDPLMTEAERHDETCLLYVAVTRALRTLVLNDLMHQLAEEQQKTEGYRG
                     TGYDISGLVQENGHA"
     gene            870..>1408
                     /locus_tag="ACHKIR_005309"
     CDS             870..>1408
                     /locus_tag="ACHKIR_005309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000893504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraI domain-containing protein"
                     /protein_id="HFO9992885.1"
                     /translation="MLERLKRLFPVKTECSDVRTDIPGETVPEGYFTPLPADVLVSTP
                     FRKRCLKQLWENSSMPVDMYQRLCLDPVHQLLSVSQNLPATAEGHWGYTGGFGDLTLQ
                     FTTYAVRLARGRMFPPGATAEEQAAQGSVWNMVVFWSALFYHLPLLAELEGEQADGRC
                     WQPGISVPEGVYRFRFRKMP"
BASE COUNT          325 a          298 c          454 g          331 t
ORIGIN      
        1 tctggggcag aagtgtcggg tggtccttgt tggagattgt tatcaacaga tttaccgctt
       61 tcggggtgcg aataatgcgc tttcccatcc tgcactgaag aatgcggacc ggttgtggct
      121 gacacagagt ttccgttttg gtccggcggt ggctcgtatg gcaaatctgc ttctgcagcg
      181 tgaaggtgaa acacgagagg tgaaaggttg tggtggtgat gatgaggtat tgctgaaatg
      241 ccatgcgcgt gaacatctgc aggggcatta cacggtactc agtcgtactg tcgcgggtgt
      301 gattggtact gcgctgatgg cggcaatgaa agggcaaaag gtttactggg tggggggaat
      361 cgaaggttac aggaccgggg aacttgagga tctctactgg ttccaggttg atatgccgga
      421 acggatgcat tcagacaggc tgcgccggga ctacaggaat tttgaagaat ataaatatat
      481 tgcaaaatcc acgaaggatg tggagatgaa tcagtccctt cggcttctgg agttgtgttt
      541 tccgttaccg aaaaaactgg aacttctccg gcagtatacg gtaacgaatg agcaggatgc
      601 agatatcacg gtatcaacgg cacaccggag caaggggctg gagtggccgg ttgtaatgct
      661 ggatgaggat ttccaggata tcacggatcc gctgatgacg gaggccgagc gtcatgatga
      721 aacctgtctg ttatatgtgg ctgtcacccg ggccctcagg acgctggtac tgaatgacct
      781 gatgcaccaa ctggcagaag aacagcagaa aactgaaggt taccggggga ccgggtacga
      841 catctcaggt ctggttcagg agaatgggca tgcttgaacg cctgaaaagg ttatttcctg
      901 tgaagacaga atgctctgat gtccggacgg atattcccgg ggaaacggtg ccggaggggt
      961 attttacacc attgccggct gatgtactgg tatccacacc gttcaggaaa aggtgtctga
     1021 aacagttatg ggaaaacagt tccatgccgg tggatatgta tcagcgcctt tgtcttgatc
     1081 ctgtgcatca gttgttgtct gtcagtcaga atctgccggc cacagcagaa ggtcactggg
     1141 gttacaccgg tggttttggt gacctgaccc tgcagttcac gacgtatgct gtccggctgg
     1201 ccagaggcag aatgtttcca ccgggagcga cagcagaaga gcaggccgcg caggggagtg
     1261 tgtggaacat ggtggtgttc tggtcggctc tgttttatca cctgccgttg ctggcggaac
     1321 tggaaggcga acaggccgac ggcagatgct ggcagccggg gatatctgta ccggagggcg
     1381 tataccggtt ccggttcagg aagatgcc
//
LOCUS       DAZGQL010000299         1357 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.303, whole genome shotgun sequence.
ACCESSION   DAZGQL010000299 DAZGQL010000000
VERSION     DAZGQL010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1357
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.303"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            49..951
                     /locus_tag="ACHKIR_005310"
     CDS             49..951
                     /locus_tag="ACHKIR_005310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase domain-containing protein"
                     /protein_id="HFO9992886.1"
                     /translation="MSRREKLLKQDLVAHIRKDKGSFKKLSDYTRIMHRFAESLAKMN
                     VQISSAAQVKVRHIELYMRSRSNVSGRTRENEMSAIRVMLRQAGKYKMADPEHPRLSN
                     KALGISGSSRKGTKKLITDERFREILQRVEAKDKGVAAVVKLSRYLGLRNEEAIQSAK
                     SLRTWKKALQRGDEKIRVIFGTKGGRERMTTVVDRDNVLDAVNYALKYASEHNGRLID
                     RDNLKSAMDYYLNTLRRYGELKGGDETAHSFRYSYAASAMKYHIMNGYSYKEALALTS
                     IDLGHGDGRGRYIEQVYCQLLGYE"
     gene            984..1307
                     /locus_tag="ACHKIR_005311"
     CDS             984..1307
                     /locus_tag="ACHKIR_005311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001350394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992887.1"
                     /translation="MDCTILLKIISVKEITKNSPLWSHYLARYVDSGYQAEFECEGTA
                     DKWDIYDEWDIYESIADYFDVVGDFNCDVRWSGRKILIKGTCVFPLGEPVKGIHNIPL
                     WIDVE"
BASE COUNT          429 a          211 c          339 g          378 t
ORIGIN      
        1 gaacgtacag ctgtttaagg taaaaattat tatgtgcagg agaagttgat gtccagacgt
       61 gaaaaattac tgaaacagga tctggttgct cacattcgaa aagacaaagg cagcttcaag
      121 aagctgagtg attacaccag gatcatgcac cgctttgctg aaagtctggc aaaaatgaat
      181 gtacagatca gttctgccgc gcaggtaaaa gtccggcata ttgagctata tatgagaagc
      241 cggagtaatg tttctgggcg aacaagggaa aatgaaatgt ctgcaatacg ggttatgtta
      301 cgtcaggcag gtaaatataa gatggctgat cccgaacatc ctcgtctcag caataaagcg
      361 ttaggcattt ccggctccag tcgtaaggga acaaaaaaat taatcacgga tgagcgtttt
      421 cgggagattc ttcagcgtgt ggaagcaaaa gataaagggg tggctgctgt tgttaaactg
      481 tcacgttacc tcggtctgag aaatgaagaa gctatccagt cggccaaatc attacgaacc
      541 tggaaaaaag cgttacaacg gggagatgaa aaaataagag tgatttttgg tacgaaaggg
      601 ggacgggaaa gaatgacaac ggttgttgac cgggataacg ttcttgatgc tgttaattat
      661 gcactcaaat atgccagtga acataatgga cgtcttatag acagagataa tcttaagtcg
      721 gcaatggatt attatcttaa tacattacgt cgttatggag aactgaaagg cggagatgag
      781 actgcacaca gttttcggta cagttacgct gcaagtgcca tgaaatatca tattatgaac
      841 ggttacagtt ataaggaggc acttgcactg acatcaattg atcttggtca tggtgatggc
      901 agaggacgat atattgaaca ggtttattgt cagttgttag gctatgagta ataactcatg
      961 gcgatttcgt acgggtaaat attatggatt gtactatttt gctgaaaata atctctgtaa
     1021 aagagattac aaaaaattcg cctttgtggt cacactatct ggcgcgatat gttgattccg
     1081 gatatcaggc cgagtttgaa tgtgaaggaa cagcagataa atgggatatc tatgatgaat
     1141 gggatatcta tgaatccatt gcggattatt ttgatgtggt gggagatttt aactgtgatg
     1201 tcagatggtc gggaagaaaa atactgatta aagggacctg tgtttttcct ctgggagaac
     1261 ccgttaaggg aatacataat attcctcttt ggattgatgt tgaataactt gacattcatc
     1321 ttttgtgatt taaaaatata caggcttata tgtcgct
//
LOCUS       DAZGQL010000300         1342 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.304, whole genome shotgun sequence.
ACCESSION   DAZGQL010000300 DAZGQL010000000
VERSION     DAZGQL010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1342
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.304"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..1182
                     /locus_tag="ACHKIR_005312"
     CDS             <1..1182
                     /locus_tag="ACHKIR_005312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFO9992888.1"
                     /translation="GTGRQWSVSGPHIKPGKDFWFYVRSVNLVGKSAFVEASGRASND
                     AEGYLDFFRGEIGKTHLAQGMWELIDNSQLDDEMAEMKTTITETRNEITQTVSKTLED
                     QSATIQQIQRVQTDTNNDLAALYMLKVQKTKNGIPYVAGIGAGIEDADGQPLSNILLQ
                     ADRIAMINPENGNTTPMFVAQGNQLFMNDVFLKRLFAVSITSSGNPPTFSLTPEGRLT
                     ARNADISGHISANSGTLNNVTINQNCRILGKLSANQIEGDIVKTVGKAFPRDSRAPER
                     WPSGTITVRIYDDQPFDRQIVIPAVAFSGAKHEREHNDIYSSCRLMVKCNGRVLFERT
                     ALAESQVYSGVIDMPAGRGHMTLEFSVSAWLVNGWYPTASISDLLVVVMKKSTAGISI
                     S"
BASE COUNT          334 a          315 c          417 g          276 t
ORIGIN      
        1 gggacaggcc gccagtggag tgtctccggc ccgcacatta agccggggaa ggatttctgg
       61 ttttatgtgc gcagcgtcaa cctggtgggg aaatctgcgt ttgtggaagc cagtggccgg
      121 gcgagcaatg atgcggaagg gtatctggac tttttcagag gagaaatcgg gaagacacat
      181 ctggcacagg ggatgtggga gctgattgat aacagccagc ttgacgatga gatggcggag
      241 atgaagacca ccatcacaga aacccgcaat gaaatcacac agacggtcag taaaaccctg
      301 gaagaccaga gcgccaccat acagcagata cagcgggtgc agacagacac aaataacgac
      361 ctggctgcgc tgtacatgct gaaggtgcag aaaacaaaaa acggcattcc gtatgttgcc
      421 ggtataggtg cggggattga ggatgctgat ggccagcccc tgagcaatat tctgctgcag
      481 gcggaccgta tcgcgatgat taacccggag aacggcaaca ccacgccgat gtttgtggcg
      541 caggggaatc agctgttcat gaacgatgtg ttcctgaagc ggttgtttgc agtgagcatc
      601 acctcgtccg gcaatccccc gacgttctcc ctgacgccgg agggcaggct gacggcccgc
      661 aatgcggaca tcagcggaca tatcagtgcg aactcgggca cgctcaataa tgtgaccatt
      721 aaccagaact gccggatact tggaaaactg tctgccaacc agattgaagg cgatattgtt
      781 aaaaccgtgg ggaaggcttt cccgcgggac tcccgcgcac cggagcggtg gccatcgggg
      841 accattaccg tcaggattta tgacgatcag ccttttgacc ggcaaattgt gatcccggca
      901 gtggctttca gcggcgctaa acatgagaga gagcataacg atatttattc atcatgccgg
      961 ctgatggtga aatgtaacgg cagggtgctt tttgaaagga cggcgctggc tgagtctcag
     1021 gtgtattcag gggtgattga tatgcctgca ggaagggggc atatgacgct ggagttttct
     1081 gtgtctgcct ggctggtgaa tggatggtac ccgacagcga gtatcagcga tctgctggtt
     1141 gtcgtgatga agaagtccac ggcgggtatc agtatttcgt gatcatgagg agccggattg
     1201 ccggaaggag acaaaaaccg tacattatgc gcgggtgcct ttggctgatg gccggaggga
     1261 gcacctgaag gccggatgtg aaaaggcccc ggacaaacat tcatgtttaa cccgaggcct
     1321 gacgttccta ccttcaacaa gt
//
LOCUS       DAZGQL010000301         1336 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.305, whole genome shotgun sequence.
ACCESSION   DAZGQL010000301 DAZGQL010000000
VERSION     DAZGQL010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1336
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.305"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..1285)
                     /locus_tag="ACHKIR_005313"
     CDS             complement(<1..1285)
                     /locus_tag="ACHKIR_005313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9992889.1"
                     /translation="MKLTINEKIGFGAGDMAIAIVMMSLAMIITYFYTDVFGLKPVDL
                     GILLFSVRILDAVIDPVVGTMTDRTNTRWGKYRPWLLFMSIPFGISIWLMFTTPNTDY
                     SVKLLWAWSTYVLLTLTYTLIAIPYVSLISVITDDPQERLSANGYRFVMTKIAMFAVT
                     IIVPLSAMYLGKNNVQLGYQIAMGAMGILATCLCLYCFFSVRERIYHPKPSLGMAAQF
                     RLLIKNDQWLILGAVIAIIMFGGIIRNSVAAYYAKYYLNGGDALISPFLTSGVIASVL
                     AMIACTWLTRLYDKIKIFRYTQLLAFVVGGAMYFVQPNSIVLAFTFYIVVTFLTDIQL
                     PIYWASIAESVDYGEMKTGQRVSGLAFGGILFFQKFGMGLAGGFIGIALAFMDYQPGV
                     EQTPQALWGICLLMTIIPAILNLITGLIMRFYLINN"
BASE COUNT          456 a          304 c          244 g          332 t
ORIGIN      
        1 cattattaat cagataaaat ctcatgatga gtccggtaat caggttaagt atcgcgggaa
       61 tgatcgtcat taacaggcag atcccccaca acgcctgtgg tgtttgttct acgccaggtt
      121 gatagtccat aaacgctaaa gcgattccga taaaccctcc cgccagtccc ataccaaact
      181 tttgaaaaaa gagaatcccg ccaaatgcca gtccagaaac acgttgcccc gttttcattt
      241 caccataatc cactgactca gcaatggatg cccaataaat gggaagctga atatcagtca
      301 gaaaggtcac cacaatataa aatgtaaaag ctaacactat agaatttggt tgcacgaaat
      361 acatcgcacc acccaccacg aacgccagta attgggtata acgaaatatt tttattttgt
      421 cgtagagtcg agtaagccag gtgcaagcga tcatagccag aacagaggcg atcacgcctg
      481 aagttaaaaa cggtgaaatt aaagcatccc caccattcag gtaatatttt gcataatatg
      541 cagcgacaga gttacgaata atcccgccaa acataatgat cgcaattaca gcgcccaaaa
      601 tgagccattg atcatttttg atcagtaacc gaaactgcgc ggccataccg agactgggtt
      661 ttggatggta gatgcgctct ctcacgctaa aaaaacaata caggcataaa cacgttgcca
      721 gaattcccat tgcccccatt gctatttgat aacccagttg tacgttattc ttcccaagat
      781 acattgcaga taaaggaaca ataattgtta cggcgaacat ggcaattttt gtcataacaa
      841 aacgataacc attggcgctc aatcgctctt gtggatcatc cgtgataacg ctgattaatg
      901 aaacataagg aatagcgatt aacgtatagg tcaaggtaag taacacatag gtagaccagg
      961 cccataaaag cttaactgaa taatcggtat taggtgtggt gaacatcaac caaatgctta
     1021 tcccaaacgg tattgacata aaaagcaacc acggccgata ttttccccaa cgtgtattcg
     1081 ttctgtctgt cattgttccc acaactggat caatcacagc atcaaggata cgtacagaaa
     1141 ataacagaat acctaagtcg acgggtttca gaccaaatac atccgtgtaa aaataagtga
     1201 tgatcatggc caaagacatc attactatcg cgatagccat atcaccagca ccaaaaccta
     1261 tcttttcatt tattgtaagt ttcatacaca gcactcattg attacgtgaa cttatattaa
     1321 aaacaaatat atctga
//
LOCUS       DAZGQL010000302         1328 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.306, whole genome shotgun sequence.
ACCESSION   DAZGQL010000302 DAZGQL010000000
VERSION     DAZGQL010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1328
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.306"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>1328
                     /locus_tag="ACHKIR_005314"
     CDS             <1..>1328
                     /locus_tag="ACHKIR_005314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFO9992890.1"
                     /translation="IVGLQVDAEQFGGQQLTVNYHIRGRIIQVPSNYDPEKRTYSGIW
                     DGSLKPAYSNNPAWCLWDMLTHPRYGMGKRLGAADVDKWALYAIGQYCDQTVPDGFGG
                     TEPRMTFNAYLSQQRKVWDVLGDFCSAMRCMPVWNGQTLTFVQDRPSDVVWPYTNSDV
                     VVDDNGVGFRYSFSALKDRHTAVEVNYTDPQNGWQTSTELVEDPDAILRYGRNLLKMD
                     AFGCTSRGQAHRAGLWVIKTELLETQTVDFTLGSQGLRHTPGDIIEICDNDYAGTLTG
                     GRILSIDAASRTLTLDREVTLPETGTSTVNLINGSGKPVRVDITAHPAPDRIQVSALP
                     DGVEAYGVWGLSLPSLRRRLFRCVSIRENTDGTFAITAVQHVPEKEAIVDNGARFEPM
                     SGSLNSVIPPAVQHLTVEVSASDGQYLALAKWDTPRVVKGVRFSLRLTSG"
BASE COUNT          268 a          376 c          445 g          239 t
ORIGIN      
        1 attgtggggc tgcaggtgga tgcggagcag tttggtggcc agcagctgac ggtgaactac
       61 catatccgtg gtcgcatcat tcaggtgccg tcaaactatg acccggaaaa acgcacctac
      121 agcggtatct gggacggcag tctgaaaccg gcatacagca ataacccggc ctggtgcctg
      181 tgggacatgc tgacccaccc gcgctacggg atgggaaaac gcctgggggc cgcggatgtg
      241 gacaagtggg cactgtatgc catcgggcag tactgtgacc agacggtccc ggatggtttc
      301 gggggaacag agccgcggat gaccttcaat gcgtacctgt cacagcagcg taaggtctgg
      361 gatgttctgg gggatttctg ctcggcgatg cgctgtatgc cggtatggaa cggccagacg
      421 ctgacgttcg ttcaggaccg tccgtcggat gtggtgtggc cgtacaccaa cagcgatgtg
      481 gtggtggatg ataacggcgt ggggttccgc tacagcttca gtgccctgaa ggaccggcac
      541 acggcggtgg aggtgaatta caccgacccg cagaacggct ggcagacttc cacggaactg
      601 gtggaagacc cggacgccat cctgcgctac gggcgcaacc tgctgaagat ggacgcgttc
      661 ggctgtacca gccgcggtca ggcccaccgt gccggactgt gggtaataaa gacagaactg
      721 ctggaaacgc agacggtgga tttcacgctc gggtcacagg ggctgcggca cacgcccggt
      781 gacatcattg aaatctgtga taacgactat gccgggaccc tgaccggcgg acgtatcctg
      841 tccatcgatg ccgccagccg cacactgacg ctggaccgtg aggtgacact gccggagaca
      901 ggtacatcga cggtgaacct gattaacggc agcggtaagc cggtgcgcgt ggacatcact
      961 gcacaccccg ccccggaccg gatacaggtc agcgccctgc cggatggcgt ggaggcatac
     1021 ggtgtgtggg gactctccct gccgtcactg cgtcgtcgcc tgttccgctg tgtttccatc
     1081 cgggaaaaca cggacggcac ctttgccatc acggcagtgc agcacgtacc ggaaaaagaa
     1141 gccatcgtgg ataacggggc ccgctttgag ccgatgtccg gttcactgaa cagcgtcatc
     1201 ccgccggctg tgcagcacct cacggtggag gtgagtgcct cagacggcca gtatctggcg
     1261 ctggcgaaat gggacacgcc gcgggtggtg aagggcgtgc gcttcagtct gcgcctgacc
     1321 agtggcag
//
LOCUS       DAZGQL010000303         1316 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.307, whole genome shotgun sequence.
ACCESSION   DAZGQL010000303 DAZGQL010000000
VERSION     DAZGQL010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1316
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.307"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            104..>1316
                     /locus_tag="ACHKIR_005315"
     CDS             104..>1316
                     /locus_tag="ACHKIR_005315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HFO9992891.1"
                     /translation="MKSIFEGLSQVTALLGTPVSAETLAAGTVRTDVSGIDFRSVGEF
                     LRSEGFDNHLSRRAPEDIPSLAVPVLLLLNAQEAIVVVRIEGAGLARQYHVLQPGGLR
                     KTLSHDELMAVYSGYCWFVQPQATQDRRSELPEYDMTGAWFWRVLWRFRPYYGQVILA
                     SFIINFLALVSSLYVMNVYDRVIPNRSWETLWMLSIGVIAAILFECAAKLIRSYLTDI
                     AGKKADLIISSALLRRVMAVKMSDRPASSGSYASNLREYESVREFMTSATLLAFVDLP
                     FILLFIGVIGLVGGWLAVVPLTLVPLVILAGILLQRPLSRHINASMRESSQRQGLLVE
                     ALDGIETLKLNNAVNWVQRRWDGYTASVARSSIRVKNTSNVIICFSTAVQQLNTVLLV
                     ILGTYLIHSDDP"
BASE COUNT          285 a          293 c          371 g          367 t
ORIGIN      
        1 atgctggatt atctgtattc ccagggaatg ctcctgaaat ggagcggagt gaatctttct
       61 ggtgcagaag aaaaagtaac agggcagaag gacagataaa taagtgaaga gtatttttga
      121 aggattatca caggtaacgg cattactggg tacacctgta tctgctgaga cgctggctgc
      181 cggtacagtc cggacagatg tctccgggat tgattttcgt tctgttggcg agtttcttcg
      241 cagtgaaggg tttgataatc atctttcccg gcgggctccg gaagatattc cttcacttgc
      301 ggttcctgtg cttcttttac tgaatgcaca ggaagccatt gtggtggtgc gtattgaggg
      361 tgccggcctg gcccggcagt atcatgtact ccagcccggg ggactccgta agacactttc
      421 tcacgatgaa ttaatggctg tttattcagg ttactgctgg tttgttcagc cacaggcaac
      481 acaggatcgc cgttcggaac tgccggagta tgacatgacc ggggcatggt tctggcgggt
      541 actgtggcgg ttcaggccat attacggaca ggtcattctt gcgagtttta tcattaactt
      601 tcttgccctg gtcagttccc tgtatgtcat gaacgtttat gaccgggtca tacccaatcg
      661 ctcctgggaa acgttatgga tgctgagtat cggggtgatt gcagccattc tgtttgagtg
      721 tgctgcaaag ttaatccgga gctatctgac ggatattgcg ggtaagaagg cagacctgat
      781 tatcagctca gcactgttaa ggcgggtgat ggcagtgaaa atgtcggaca ggccggcgtc
      841 ttccggttca tacgccagca atttgcggga atatgagtct gtcagggagt ttatgaccag
      901 cgccacgctg ctggcttttg tggatttgcc gtttatcctg cttttcatcg gtgtcatcgg
      961 gcttgtgggg ggctggcttg ctgttgttcc tcttacactg gttccgctgg tgattctggc
     1021 aggaatatta ctgcagcgac cgctttcgcg tcatattaat gcctccatgc gggaatcatc
     1081 gcagcgccag ggattacttg tggaggcact ggacggaata gaaaccctga agctgaataa
     1141 tgcagttaac tgggtccagc gccgctggga tggatataca gccagtgtcg cccgctcgtc
     1201 catacgggta aaaaatacca gtaatgtcat aatctgcttt tccacagcag ttcagcaact
     1261 gaatactgtt ctgctggtca ttctggggac gtaccttatt cacagtgatg acccac
//
LOCUS       DAZGQL010000304         1291 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.308, whole genome shotgun sequence.
ACCESSION   DAZGQL010000304 DAZGQL010000000
VERSION     DAZGQL010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1291
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.308"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            83..643
                     /locus_tag="ACHKIR_005316"
     CDS             83..643
                     /locus_tag="ACHKIR_005316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992892.1"
                     /translation="MKEEPEIIEINSEEFTENEWLTETDPRVLALTMEGYIVFPPFFS
                     DAEYSAINNAGIDRAFHKYELDCGPVYRVCRHGQVKKCFTRKTAIIHLAHFMTTKVFE
                     SSGFEKRLPDQCLMHPEYGEVFHPGEITPEYYRAHGRCFHRLLRILARRKQFDAWMEK
                     YNAWIDQYCRLVAQRPFKKRANHDCN"
     gene            630..1214
                     /locus_tag="ACHKIR_005317"
     CDS             630..1214
                     /locus_tag="ACHKIR_005317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992893.1"
                     /translation="MTATEQLFFLTAEGIHTPYGLLDINKALEMLDEGCFDEPIWHGL
                     EIVCCISEAEEKGMTGELAPEKTIMYWRWLYTAMFFSEQLTRNGWTEVLNENGGTDKG
                     TMYRGHNGGMTIYLKPLRLAMQNYMEWALIETYGAESGLLKSILMYKAFTGALGDGEK
                     TGITMEGYRAMHMILDGLISEATDNSEPETRVFH"
BASE COUNT          395 a          293 c          309 g          294 t
ORIGIN      
        1 acaaacgaat tgctgagcca catcttctgg ctgcgctcag agtgaataaa agtaattaac
       61 accggatgaa cacggagtaa ccatgaaaga agaacctgaa atcattgaaa ttaattctga
      121 agaattcaca gaaaacgaat ggctgacaga aaccgatccg cgcgttctgg cgctgacaat
      181 ggaaggatac atcgtatttc cacctttctt cagtgatgcg gaatacagtg caataaataa
      241 tgccggaatc gaccgtgctt ttcataaata cgaactcgat tgcggccctg tttacagagt
      301 ctgccgccac ggacaagtta agaaatgctt cacgcgaaaa acggccatca tacatctcgc
      361 tcattttatg acaacgaagg tattcgaaag ttcaggtttt gaaaaacgtc tgcctgacca
      421 gtgcctgatg catccggaat atggagaagt ctttcatccg ggagaaatta cgcccgaata
      481 ctacagagct cacgggcgtt gttttcatcg cctgttgcgc atccttgcac gcagaaaaca
      541 gtttgatgcc tggatggaaa aatacaacgc ctggattgat cagtattgcc ggcttgtcgc
      601 gcaaagacca tttaagaaga gagcaaacca tgactgcaac tgaacagtta ttttttctta
      661 ccgctgaagg tatccacaca ccttacggac tgcttgatat caacaaagca ctggaaatgc
      721 tcgatgaagg ctgttttgac gagccgatct ggcatggtct ggaaatagtg tgttgcatca
      781 gtgaagcaga agaaaaaggc atgacaggtg agctcgcgcc ggaaaagaca atcatgtact
      841 ggcgctggct ttataccgcc atgttcttct ctgaacagct caccagaaac ggctggaccg
      901 aagtgcttaa cgagaatggc ggtacagaca agggcaccat gtacagaggc cacaacggtg
      961 gcatgacaat ctacctgaaa ccattacgtc tcgctatgca gaactatatg gaatgggcac
     1021 tcatagaaac ttacggagcg gaatcagggc tgctcaaaag cattcttatg tacaaagcat
     1081 tcaccggcgc tctgggcgac ggcgaaaaaa ccggcatcac aatggaaggt tacagagcga
     1141 tgcacatgat tctggatggg cttatcagtg aagccactga caactcagaa ccggaaacca
     1201 gagtgttcca ctaatcagct tttcgtgagg tgtaatgatg aaaaaatcaa ctgaacttgt
     1261 gccggtggca gcccgtgatt tgcaaattat c
//
LOCUS       DAZGQL010000305         1273 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.309, whole genome shotgun sequence.
ACCESSION   DAZGQL010000305 DAZGQL010000000
VERSION     DAZGQL010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1273
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.309"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..152
                     /locus_tag="ACHKIR_005318"
     CDS             <1..152
                     /locus_tag="ACHKIR_005318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004717867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HFO9992894.1"
                     /translation="SDIYVGTKDIAEQLVAQSVAGKIVALENMIDKKAMRERLSVALT
                     ELGAL"
     gene            165..>1273
                     /locus_tag="ACHKIR_005319"
     CDS             165..>1273
                     /locus_tag="ACHKIR_005319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011815933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFO9992895.1"
                     /translation="MDFFRFLMSDVLSEPAVLVGLIALIGLIAQKKPVTECIKGTVKT
                     IMGFVILGAGAGLVVSSLSDFANIFQYAFGIQGVVPNNEAIVSVAQKSFGKEMAMIMF
                     FAMVINIMIARFTPWKFIFLTGHHTLFMSMMVAVILATAGMTGITLIAVGSLVVGVAM
                     VFFPAIAHPYMKKVTGSDDVAIGHFSTLSYVLAGFIGSKFGNKEHSTEDMNVPKSLLF
                     LRDTPVAISFTMSIIFLVTCLFAGADAVKELSGGKNWFMFSIMQSITFAAGVYIILQG
                     VRMVIAEIVPAFKGISDKLVPNARPALDCPVVFPYAPNAVLVGFLSSFAAGLIGMFTL
                     YLLNMIVIIPGVVPHFFVGAAAGVFGNATGGRRGA"
BASE COUNT          280 a          272 c          344 g          377 t
ORIGIN      
        1 cgagtgatat ctatgtcggc accaaagata ttgccgagca gttagtcgcg cagtctgtcg
       61 caggcaaaat cgtcgcactg gaaaatatga ttgataagaa agccatgaga gaacgtttat
      121 ccgtggcatt aaccgaatta ggcgcactgt aagctggggg cgatatggat ttctttcgtt
      181 ttttgatgag cgatgtactt tccgaacccg cagtgctggt cggtttaatc gccctgattg
      241 gcctgattgc acagaaaaaa ccggtaaccg aatgcattaa aggtaccgtc aagaccatta
      301 tggggtttgt gattttaggc gcaggggcgg gtttagttgt gtcgtcactg agtgacttcg
      361 caaacatttt ccagtatgcc tttggtattc agggtgtagt gccgaacaat gaagccattg
      421 tttccgtagc gcagaaaagc ttcgggaaag aaatggcgat gattatgttc tttgcgatgg
      481 ttatcaacat tatgattgcc cgtttcacac cgtggaaatt tatcttcctg accggtcatc
      541 acacgttgtt tatgtcgatg atggtagcgg taattctggc aacagcaggc atgaccggca
      601 ttacgcttat agccgttggc tctttggttg ttggcgtggc aatggtcttt ttcccggcta
      661 ttgcgcatcc gtacatgaag aaagtgaccg gttctgatga cgttgccatc ggccacttct
      721 caacattatc ttatgtattg gccggtttca tcggcagcaa gtttggtaat aaagagcact
      781 cgactgaaga catgaatgtg ccgaaaagtc tgctgttctt gcgcgacacg ccggttgcta
      841 tctcttttac catgagcata attttcctgg tgacgtgcct gtttgcgggt gcagatgcgg
      901 tgaaagagct tagcggtggt aaaaactggt tcatgttctc tatcatgcaa tccattacct
      961 tcgcagctgg cgtgtacatc attctgcagg gtgtgcgcat ggtgattgcg gagattgtcc
     1021 cggcattcaa aggtatttca gacaagctgg ttccgaatgc cagacctgct ctcgattgcc
     1081 cggtcgtctt cccttacgca cctaacgctg tactggttgg tttcctgagt agctttgctg
     1141 caggtttaat cggtatgttc acgttgtatc tgctgaacat gatcgtgatt attcctggtg
     1201 tggtgccgca cttcttcgta ggtgcagctg caggggtatt cggtaatgca acgggtggac
     1261 gtcgtggtgc gat
//
LOCUS       DAZGQL010000306         1242 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.310, whole genome shotgun sequence.
ACCESSION   DAZGQL010000306 DAZGQL010000000
VERSION     DAZGQL010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1242
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.310"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>1242
                     /gene="tssC"
                     /locus_tag="ACHKIR_005320"
     CDS             <1..>1242
                     /gene="tssC"
                     /locus_tag="ACHKIR_005320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017892320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HFO9992896.1"
                     /translation="LIDHHIAELDDQISRQLDAVLHHPEFQKVESLWRGVKSLVDKTD
                     FRRNVKIELLDLSKDDLRQDFEDAPEIIQSGLYLQTYVAEYDTPGGEPIAALVSAWEF
                     DASAQDVALLKNISRVAASAHMPFIGSVGPAFFRKETMEEVAAIKDIGNHFERAEYIK
                     WNAFRETDDARYIGLVMPRVLGRLPYGPDTVPVRSFNYVEEVKGPDHHKYLWTNASFA
                     FAANMVRSFVTNGWCVQIRGPQAGGAVQDLPIHLYDLGTGNQVKIPSEVMIPETREFE
                     FANLGFIPLSYYKNRDYACFFSANSTQKPALYDTPDATANSRINARLPYIFLLSRVAH
                     YLKMIQRENIGTTKDRRLLELELNTWLRGLVTEMTDPGDELQASHPLRDGKVVVEDIE
                     DNPGFFRVKLYAIPHFQVEGM"
BASE COUNT          270 a          293 c          358 g          321 t
ORIGIN      
        1 gctgattgac catcatattg cagagcttga tgaccagatc agtcgtcagc tggatgccgt
       61 gcttcatcat ccggaatttc agaaagtgga atccctgtgg cggggcgtga agtctctggt
      121 ggataaaacg gacttccgcc gtaatgtgaa aattgagctg ctggatctgt ccaaagacga
      181 tctgcgccag gattttgagg atgcgccgga aattattcag agcggccttt atctgcagac
      241 ctatgtggct gaatatgaca cgccgggtgg tgaacccatt gctgccctgg tttcggcctg
      301 ggaatttgat gcatctgcgc aggatgtggc gttgctgaaa aatatttccc gggtggcagc
      361 atccgcacat atgccgttta ttggttctgt tggtccggct ttcttccgga aagaaaccat
      421 ggaagaggtg gctgctatta aggatattgg caatcacttt gagcgtgccg agtacatcaa
      481 gtggaacgct ttccgagaga cggatgatgc ccgctatatt ggtctggtga tgccccgtgt
      541 gctgggacgc ctgccgtatg gccctgatac tgtacctgtc cgcagcttta actatgttga
      601 agaggtgaaa gggccggatc atcacaagta tctctggacg aacgcgtctt ttgcttttgc
      661 ggccaatatg gtgcgcagtt ttgtcaccaa cggctggtgt gttcagatcc gcggtcctca
      721 ggccggtggt gcggtgcagg atttgccgat ccatctgtat gatctgggaa ccggtaatca
      781 ggtcaaaatt ccgtcagagg tgatgatccc ggaaacgcgg gagtttgaat tcgctaatct
      841 gggttttatt ccgctctctt actacaagaa ccgggattat gcctgtttct tctccgctaa
      901 ctccacccag aaaccggcac tgtacgatac gccggatgca acggcgaaca gccgtattaa
      961 tgcccgtctt ccctatattt tcctgctgtc ccgcgttgcg cattacctga agatgattca
     1021 gcgggaaaat atcggtacca ccaaagatcg gcgtctgctg gagctggagc tgaatacctg
     1081 gctccggggc ctggtgacgg agatgaccga tccgggcgat gagcttcagg catcgcatcc
     1141 gctgcgtgac ggaaaagtgg tggtggaaga tattgaagat aacccggggt tcttccgtgt
     1201 gaaattgtat gccattccgc atttccaggt ggaaggaatg ga
//
LOCUS       DAZGQL010000307         1242 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.311, whole genome shotgun sequence.
ACCESSION   DAZGQL010000307 DAZGQL010000000
VERSION     DAZGQL010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1242
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.311"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..399)
                     /locus_tag="ACHKIR_005321"
     CDS             complement(<1..399)
                     /locus_tag="ACHKIR_005321"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992897.1"
                     /translation="MTTCSELKERARFDGMHDASLEVAWQLLMTKSVSTITTEIISGA
                     DMSNFSVIIRERYKHAIAKSLLNHLSPDVVSDIIGLSHDEFNQQYKGNLTRQQHLRDI
                     AVSLTDKGFTESSACNIVNLDLMLLPEENWL"
     gene            complement(483..>1242)
                     /locus_tag="ACHKIR_005322"
     CDS             complement(483..>1242)
                     /locus_tag="ACHKIR_005322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III restriction-modification system
                     endonuclease"
                     /protein_id="HFO9992898.1"
                     /translation="YIHNDTAMSKSIVSDDDNFAKLNTMSYREFLDNLSQTIFAKHDT
                     LHKVFCDIKDTINITDYLNIQTIRKIKSGFSKYLLNNSFNKFSLGYNVISGSIHPTKF
                     TNADGNHLHEVLSGDLGVLQDNTKAPLDSYLFEEVFYDSELERLNITDGEIHSVAVFT
                     KIPKNSIKIPVAGGYTYSPDFAYVVKTTKGDYLNFIIETKNVEGKESLRKEELDKIKH
                     AQKLFEQVSKSVKVTFITQFSGDRIHDLIKNVIQ"
BASE COUNT          368 a          213 c          221 g          440 t
ORIGIN      
        1 taaccagttt tcttcaggaa gtaacattag gtccaggttg acaatattac atgcagatga
       61 ttctgtgaat cctttatctg ttaaacttac agcaatatct ctcaggtgtt gttgtctggt
      121 taaattacct ttatattgtt gattgaactc atcatgagat aaaccgatga tgtcagaaac
      181 aacatcggga gataaatgat tgagaagaga ttttgctatt gcatgtttat atcgttccct
      241 gataatcact gaaaaattgg acatgtctgc accggatatt atctcagtag tgatagtgga
      301 tacacttttc gtcataagta attgccatgc cacttcaaga gaagcatcat gcataccatc
      361 aaaacgggct ctttccttca gttcagaaca tgttgtcatt gaactcacct gcgaattttg
      421 tgatgtgtgt ataattataa agtgggggtg aaaccacctc cactttgtta tcatgcatgt
      481 gtttactgaa tcacattttt tatcagatca tggattctgt cacctgagaa ttgagttatg
      541 aaagttactt ttacagattt acttacctgt tcgaaaagtt tttgtgcatg ctttatttta
      601 tccagttctt ctttacgtaa actttcttta ccttcgacat tttttgtttc aataataaag
      661 ttaaggtagt caccctttgt tgtttttaca acataagcaa agtcaggaga atatgtgtat
      721 ccacctgcca ccggaatttt aattgagttt ttcggtatct ttgtaaaaac agcaacagag
      781 tggatctctc cgtctgttat attgagtctt tcaagctctg aatcataaaa aacttcttca
      841 aaaagataag agtccagggg ggcttttgta ttatcctgta atactccaag atcacctgat
      901 aatacctcat gcaggtgatt accgtctgca ttagtgaatt ttgtaggatg aattgagcct
      961 gaaatcacat tatatccaag gctgaatttg ttaaatgaat tgttcaacaa atacttgcta
     1021 aacccagatt taatctttct gattgtctga atgttcaggt agtcggtgat attgatagtg
     1081 tcttttatat cacaaaaaac cttatgtaag gtatcgtgtt tagcaaaaat agtttgtgat
     1141 aagttatcaa gaaattcccg atagctcatt gtgtttagtt tagcaaagtt atcatcgtca
     1201 ctgacgatac ttttcgacat cgccgtatca ttatgaatat aa
//
LOCUS       DAZGQL010000308         1216 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.312, whole genome shotgun sequence.
ACCESSION   DAZGQL010000308 DAZGQL010000000
VERSION     DAZGQL010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1216
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.312"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(46..279)
                     /locus_tag="ACHKIR_005323"
     CDS             complement(46..279)
                     /locus_tag="ACHKIR_005323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HFO9992899.1"
                     /translation="MPGCTARLLPEGPFSRGQAVAVTTAYRNVFTEDDQGTYFRLVIR
                     NAEGQLRWRCWNFESDAGKQLNVWLSSEGLLRQ"
     gene            complement(350..>1216)
                     /locus_tag="ACHKIR_005324"
     CDS             complement(350..>1216)
                     /locus_tag="ACHKIR_005324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFO9992900.1"
                     /translation="HLGHDNNGGIARGATPESSGSYGFVRLEGDLLITEVAGMSLTTG
                     VYGAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYMNLTHTSSGLWADIVAQGTRHSMKA
                     SSGNNDFRARGWGWLGSLETGLPFSITDNLMLEPQLQYTWQGLSLDDGQDNAGYVKFG
                     HGSAQHVRAGFRLGSHNDMNFGKGTSSPAPLRDSAKHSVRELPVNWWVQPSVIRTFSS
                     RGDMRVGTSTAGSGMTFSPSQNGTSLDLQAGLEARVRENITLGVQAGYAHSVSGSSAE
                     GYNGQATLNVTF"
BASE COUNT          257 a          360 c          347 g          252 t
ORIGIN      
        1 gcgtctcccg gagaagatgc ggcctgatgg atgaaatgaa gacgtttatt gcctgagaag
       61 cccttcactg gagagccaca cattaagctg ttttccggcg tcagactcaa aattccagca
      121 ccgccagcgt agctgccctt cggcattacg gataaccagc cggaaatatg ttccctggtc
      181 atcttcagta aacacattgc ggtaagctgt tgtgacggca acagcctgtc cgcgtgagaa
      241 agggccttcg ggcaataaac gtgctgtaca acccggcata tctgttgctc cctgaaagta
      301 aaaagccccg gtcatgatga ccggggcctg aaggggagtg acctgattat cagaaagtca
      361 cattcagcgt ggcctgaccg ttatagcctt cagcgctgct gccgctgacg ctgtgggcat
      421 aaccggcctg aacgcccagg gtgatatttt cccggacacg ggcttccagt ccggcctgca
      481 ggtccagtga cgtgccattc tgggacggtg agaacgtcat cccgctgcct gcggtggagg
      541 tccccacacg catatcaccc cgggagctga aggtgcggat aacggaaggc tgtacccacc
      601 agttcaccgg cagttcacgc acactgtgtt ttgcactgtc acgcaggggg gcaggggatg
      661 aggtgccttt accaaagttc atatcgttgt ggctgcccag acggaagccg gcacgcacat
      721 gttgtgcact gccatgcccg aacttcacat aaccggcgtt atcctggccg tcatccaggg
      781 aaagtccctg ccaggtgtac tgcagttgtg gctccagcat caggttgtca gtgatactga
      841 agggcagacc ggtttccagt gagcccagcc agccccagcc ccgtgcgcgg aagtcgttat
      901 tgcccgatga cgctttcata ctgtggcggg ttccctgtgc cactatgtca gcccacaggc
      961 cggaggaggt gtgtgtcagg ttcatatatc cgcccaggct gccggcatca tcccggaccg
     1021 tgccggcgcg ggagccgtca tcatccttaa catcaacgga agaatggcct gcagcaccat
     1081 acaccccagt cgtcagtgac ataccggcga cctctgttat gagcaggtca ccctccagac
     1141 ggacgaagcc atagctgccg ctgctttccg gcgtggcccc acgggcaata ccgccgttgt
     1201 tatcgtgacc gagatg
//
LOCUS       DAZGQL010000309         1210 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.313, whole genome shotgun sequence.
ACCESSION   DAZGQL010000309 DAZGQL010000000
VERSION     DAZGQL010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1210
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.313"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..183)
                     /gene="mchB"
                     /locus_tag="ACHKIR_005325"
     CDS             complement(<1..183)
                     /gene="mchB"
                     /locus_tag="ACHKIR_005325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001375214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin H47"
                     /protein_id="HFO9992901.1"
                     /translation="MREITESQLRYISGAGGAPATSANAAGAAAIVGALARIPGGPLG
                     VVVGAVSAGLTTAIGST"
     gene            complement(211..420)
                     /gene="mchI"
                     /locus_tag="ACHKIR_005326"
     CDS             complement(211..420)
                     /gene="mchI"
                     /locus_tag="ACHKIR_005326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin H47 immunity protein MchI"
                     /protein_id="HFO9992902.1"
                     /translation="MSYKKLSQLTAIFSLPITILLVSLSSLRIVGEGNSYVDVFLSFI
                     IFLGFIELIHGIRRILVWSGWKNGS"
     gene            834..1079
                     /locus_tag="ACHKIR_005327"
     CDS             834..1079
                     /locus_tag="ACHKIR_005327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992903.1"
                     /translation="MNCDNNHRNEEFIVTFDKGNKQDNSRRKHDNFPIEVESSVEQET
                     HCITNNKSASGIVTHDYDADYICGCGEIMCPGCGHDL"
BASE COUNT          412 a          245 c          221 g          332 t
ORIGIN      
        1 ggtcgagcca attgctgttg tcaaaccggc agatacggct ccaactacaa ccccaagtgg
       61 accaccaggt attctggcga gagctccaac aatagctgca gcacctgcag cattagctga
      121 agtcgctggc gcacctcccg ccccggaaat atatcttaac tgtgattctg ttatttctcg
      181 catataaact gcatataaac tccatcatat ttaacttccg tttttccagc ctgaccagac
      241 cagaatcctt cgaatcccat gaatcagctc aataaaacca agaaatatta taaagcttag
      301 aaaaacgtca acataagaat tcccttcgcc aacaatccga agggatgaaa gtgaaaccaa
      361 taagatagta ataggtaaac tgaatatagc cgtcaattgg gacagttttt tataactcat
      421 taatccctac ctgttgcttt attttcatta tgaaaagtta ccaaatctta gcacctcagt
      481 caggagagtc aggtcatcga taaagaatat tccagtagag tggcgctcat gatcaaagat
      541 aacagcaaag ttgatgacag cgctgaccga ctgtcattta cagaaaccct gtttgtagca
      601 cattccatct tacctgaccc agacaaagac aaagacaaaa gagaatgatt gttatttact
      661 tttcaaaaaa aagtgtcata ttgtccatat aaagtcaaga ggtattttac gttatgtcac
      721 tgatgcaatt gttagtcagg atctcatggt tgtcgctcat gtataacctg agacttgaac
      781 gcacactaat agcactgacg gtcatacatc gggaaatgtg aggaggcata agaatgaatt
      841 gtgataataa tcacagaaat gaagaattca ttgttacctt tgataaaggc aacaagcaag
      901 acaattcaag acgaaaacac gataattttc ctatagaggt agaatcctcc gtagagcagg
      961 agacacactg tatcacaaat aataagtcgg cttccggtat agtaacacat gactatgatg
     1021 ccgattatat ttgtggttgt ggtgaaatta tgtgtcctgg ttgcggtcat gacctataac
     1081 aaacattttt gcaaactgat acctgatcag gtacaagcct gttgattatt atgctgaata
     1141 tttcatgtta taaaaccatc gatataaaca ttaacaagaa aaaacatata ttgtttatct
     1201 gaacatttat
//
LOCUS       DAZGQL010000310         1205 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.314, whole genome shotgun sequence.
ACCESSION   DAZGQL010000310 DAZGQL010000000
VERSION     DAZGQL010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1205
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.314"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            220..1164
                     /gene="leuO"
                     /locus_tag="ACHKIR_005328"
     CDS             220..1164
                     /gene="leuO"
                     /locus_tag="ACHKIR_005328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HFO9992904.1"
                     /translation="MPEVQTDHPETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQTWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
BASE COUNT          324 a          251 c          273 g          357 t
ORIGIN      
        1 gttttcgcaa caaaaacatt atggattatt atgctgtggt aaatgacgct ttccacggca
       61 atggattctg tttttatcag aacccgtatc tttatgtttt cctaatttta ctcattttcc
      121 tttttcttat tttatatgca tgataaatca tattctcgag gattatttct ctgcattcca
      181 ataagggaaa gggagttaag tgtgacagtg gagttaagta tgccagaggt tcaaacagat
      241 catccagaga cggcggagtt aagcaagcca cagctacgca tggtcgatct caacttatta
      301 accgttttcg atgccgtgat gcaggagcaa aacatcaccc gtgccgctca tgttctggga
      361 atgtcgcaac ctgcggtcag taacgctgtt gcacgcctga aggtgatgtt taatgacgag
      421 ctttttgttc gttatggccg tggtattcaa ccgactgctc gcgcatttca actttttggc
      481 tcagttcgtc aggcattgca actagtacaa aatgaattgc ctggttcagg ttttgaaccc
      541 gcgagcagtg aacgtgtatt tcatctttgt gtttgcagcc cgttagacag cattctgacc
      601 tcgcagattt ataatcacat tgagcagatt gcgccaaata tacatgttat gttcaagtct
      661 tcattaaatc agaacactga acatcagctg cgttatcagg aaacggagtt tgtgattagt
      721 tatgaagact tccatcgccc tgaatttacc agtgtgccat tatttaaaga tgaaatggtg
      781 ttggtagcca gcaaaaatca tccaacaatt aaaggcccgt tactgaaaca tgatgtttat
      841 aacgaacaac atgctgcggt ttcgctcgat cgtttcgcgt catttagtca aacctggtat
      901 gacacggtag ataagcaagc cagtatcgcg tatcagggca tggcaatgat gagcgtactt
      961 agcgtggtgt cgcaaacgca tctggtcgct attgcgccgc gttggctggc tgaagagttc
     1021 gctgaatcct tagagttaca ggtattaccg ctgccgttaa aacaaaacag cagaacctgt
     1081 tatctctcct ggcatgaagc tgccgggcgc gataaaggcc atcagtggat ggaagagcaa
     1141 ttagtctcaa tttgcaaacg ctaactgaat gcagaatagg tcagacatta atgtctggtt
     1201 tattc
//
LOCUS       DAZGQL010000311         1191 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.315, whole genome shotgun sequence.
ACCESSION   DAZGQL010000311 DAZGQL010000000
VERSION     DAZGQL010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1191
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.315"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(158..724)
                     /locus_tag="ACHKIR_005329"
     CDS             complement(158..724)
                     /locus_tag="ACHKIR_005329"
                     /inference="COORDINATES: protein motif:HMM:NF023154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFO9992905.1"
                     /translation="MYTESNVKLSCNALWQKCVLATLYRAAATHPEIKLLQFKLIPGQ
                     GINKAIPSLMADFTGELHRLISEYLQCKRRKDQSVEPTPLYYLWSREAVANDTTPYLM
                     VLLLNNDVWCKSREVVVLRNLIEQAWGKVLGEDNSITHLVVYPEVLSLVFDAANTYNA
                     EPGVLEQTARAYIESHGTIERHLGCSDF"
BASE COUNT          338 a          291 c          272 g          290 t
ORIGIN      
        1 gtggtactgc tttgatgcgc ccggtgacac aaagcccgga ttgttccaga catcctgaat
       61 caaacgcccc agattagggg cgtcgaaata tgcctctctg accattatat tccggcgtac
      121 aggtagcagg tcagaagtga caatgcgtca cctgacgtta aaagtcacta cacccaagat
      181 gacgttcaat ggtaccatgt gattcaatgt aagcccgggc cgtctgttcc agtacaccag
      241 gctcagcgtt gtatgtgtta gctgcatcaa ataccaacga cagcacttca ggatacacaa
      301 ccagatgtgt aatggagtta tcttcaccca atacttttcc ccacgcctgc tcaatcagat
      361 ttctgagaac caccacctca cgactcttac accagacatc gttattaagt agcagcacca
      421 taagataagg agtggtatcg ttagccacag cttccctact ccagagataa tataaagggg
      481 tgggctcaac agattgatct ttacgtcgct tacactgcaa atattcagaa atgagtctat
      541 gcagttcacc agtaaaatca gccatcagag agggaatggc cttattaata ccctgccctg
      601 gtattaattt aaattgtaat aatttaattt caggatgtgt ggctgcagcc cgatacagag
      661 ttgcaaggac acacttttgc cagagggcgt tacaggaaag cttaacattt gattctgtat
      721 acataataaa tcaccttaca gttacaacag gtcaaaaacc gctgtagcca gagttacgct
      781 ggcctgatgc tttagtaccg ggcatcgtct gataatccag acgctccaat aagcgctgat
      841 actgctcagg gaaatcagga tcatgaatat cctggatgtc acgtccatta gcagggaaat
      901 aaacaatgca gccccctgga ttaactatgc agaaatcgtc ctgaggcact gataaatacg
      961 gagaggactc tcgcgtatgg ttggttgaca ccacagtgcg gattcggcga atccgcgatc
     1021 acggtgcgat ttcgcaccac agtacacaat caataccccg ggttttattc aggtaagcag
     1081 gactgcggat atccggtgtc gcgcctttgt gtcacgaacg gggtgggtgc gaaacaccag
     1141 ataaaatgca ggttccgata cctctgaacg ccctgagcag gtcgggcatt t
//
LOCUS       DAZGQL010000312         1183 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.316, whole genome shotgun sequence.
ACCESSION   DAZGQL010000312 DAZGQL010000000
VERSION     DAZGQL010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1183
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.316"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..524
                     /locus_tag="ACHKIR_005330"
     CDS             <1..524
                     /locus_tag="ACHKIR_005330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO9992906.1"
                     /translation="QMNEGWYQIRIAGEDTAPEAVYACLHEQLGEGAVIHIVPRLAGA
                     GGNGVFQVVLGVAAIVGSFFTGGATLAAWGAALSAGGLTATTMLFSLGASMILGGVAQ
                     MLAPKAKTPEYKSTDNGKQNTYFSSLDNMIAQGNPMPVPYGEMLVGSRRISQDISTRD
                     EGGDGKVVVIGRG"
BASE COUNT          324 a          235 c          357 g          267 t
ORIGIN      
        1 gtcagatgaa cgaaggctgg tatcagatac gtattgccgg tgaggatacc gcgccggagg
       61 cggtgtatgc ctgtcttcac gaacaactgg gtgagggagc ggtcatccac attgtgccgc
      121 gactggccgg agccggggga aatggtgttt ttcaggtggt gctgggggtt gcagcgattg
      181 tcgggtcatt tttcactggt ggtgcaacac tggcggcatg gggcgcagcc ctgagtgccg
      241 gagggctgac tgccaccacg atgctgttct cactgggtgc cagcatgata ctgggtggtg
      301 tggcccagat gctggccccg aaggcaaaga cgccggagta caaaagtacg gataacggta
      361 aacagaacac gtatttttcg tcactggaca acatgattgc ccagggtaac ccgatgccgg
      421 tgccttacgg tgaaatgctg gttggttcac gacggatatc ccaggacatc agcacccgtg
      481 atgagggcgg agacgggaaa gtggtggtta tcgggcgggg ataaaaataa aaaaaatccc
      541 gcagagttag cggagctgcg ggaaataacg atgaagatta acgttatgga gttatttttt
      601 tgggcagaaa aacattaacg cagcgaaatt ataagcgcta catggatttt gcgaaaatgt
      661 gaagaaattc agaaattttt tgctgcagag gtgcagggag cttgccgggt atttgtggca
      721 gaagacagaa aacatcatgc cggggttaag ggataaaaaa atcccgcaga gtccggagac
      781 tgcgggacag aaccgatgaa gattaacgtt caaggagttt tgtttttatt gacccgaaaa
      841 aactgtaacg ccccggaatg atatctgcca cggggacgta cagaaaatgt gaagaaattc
      901 agactttttc tgtcgcaggt ttacagaacg gatgaccgcc agggtaccag tggcgtaaat
      961 gagcaggatg ttgtggtgtg gctgaagcaa aaaaatctcc cgtaatatcg ccggatactg
     1021 acgggagacg aatagtcacc ttaaggaggt ctgataatta ttgcttttat tgagcaaaaa
     1081 aactgtaacg ctgcgtcatt atagtggcta caggcaattg ccggaaatgt gaagaatttc
     1141 agaaatttta ttccgtcatg acacaggcac cctccggggt gcc
//
LOCUS       DAZGQL010000313         1144 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.317, whole genome shotgun sequence.
ACCESSION   DAZGQL010000313 DAZGQL010000000
VERSION     DAZGQL010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1144
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.317"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..584
                     /locus_tag="ACHKIR_005331"
     CDS             <1..584
                     /locus_tag="ACHKIR_005331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VasL domain-containing protein"
                     /protein_id="HFO9992907.1"
                     /translation="EQQLRALLAPLPDMLSADQMAQLSRTPSLLNHASDWITLSGQQI
                     TRLTELPLTYNLQRSAQLLQQLMVLFPDNPRVQEMVDNWQKSVRSRALPEEAMTGWNE
                     GMTRLQQLAERLNRLDEQRGKYMTVSELKTEVFGIMQAFNRHIPAEEQLRRYDEVRNQ
                     NGSESQQKLAQDALMEQLNRYWLLRHGDAGNPA"
     gene            566..790
                     /locus_tag="ACHKIR_005332"
                     /pseudo
     CDS             566..790
                     /locus_tag="ACHKIR_005332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
     gene            793..1079
                     /locus_tag="ACHKIR_005333"
                     /pseudo
     CDS             793..1079
                     /locus_tag="ACHKIR_005333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005687610.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          323 a          235 c          313 g          273 t
ORIGIN      
        1 cggagcagca actcagggcg ctgctggcac cactaccgga tatgctttca gcagaccaga
       61 tggcacagct cagcagaaca ccctcactgt taaatcatgc gtcagactgg ataacacttt
      121 caggtcagca gataacgcga ctgacagaac taccgctgac atacaacctg cagcgtagtg
      181 cgcagctgtt gcagcaactg atggtacttt tccccgataa tcctcgtgta caggaaatgg
      241 tggataactg gcagaagagc gtaaggagtc gtgcgctccc ggaagaggcg atgacgggct
      301 ggaatgaagg catgacccgc ttacagcagc tggctgaacg cctgaaccgc ctggatgaac
      361 agcggggaaa atacatgacg gtcagtgaac tgaaaacgga ggtgtttggc atcatgcagg
      421 cttttaaccg gcacataccg gcagaagagc agttacgtcg ctatgatgaa gtccgtaatc
      481 agaatggcag cgaatctcag cagaaactgg cgcaggatgc tctgatggaa cagttgaacc
      541 gctactggct tctgagacat ggagatgctg ggaatccggc gtgacttggg ttcgccaacc
      601 accggaagac gccattacgg atatggcgta ttttcccttt taccggtatg ccatggcctt
      661 tgaaggattc attccggcat tttcatcttg gtttgcaggt aaccggtgtg gagtggcaat
      721 actgaccggt gtccgggcgg atgaatccct caactgcttt atggggctgg tgtctcagcg
      781 caaactgaga gaattacgtg ggtaggatca gatgatggta ttggaactga aggtgatatg
      841 ggaaaatggt tgttattatt ttgtctgctg tagctcagtc tgaatgacaa catatacttg
      901 aaagaacaaa tgaaggccgt ctcgaagccg aagtaaaggg tatttaattt gggcgaaaaa
      961 gaacggtcaa cagggaacaa gtacaaacct tacgaagtct gggtatcggc gctacagaaa
     1021 tttcgcgaaa gatgaaattt acacgttcta ctgtgtataa aatattagag ggtaaataat
     1081 attttcatat ggagggttac atgaaacgta tttcagtaaa tgttttagat ttatatttag
     1141 atag
//
LOCUS       DAZGQL010000314         1140 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.318, whole genome shotgun sequence.
ACCESSION   DAZGQL010000314 DAZGQL010000000
VERSION     DAZGQL010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1140
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.318"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            131..388
                     /locus_tag="ACHKIR_005334"
     CDS             131..388
                     /locus_tag="ACHKIR_005334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020884766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HFO9992908.1"
                     /translation="MLHIIRKGDKTTHGGSVLTASETMKFGGIGVARKGDKVSCPGHG
                     PTIIIEGNPDYLDHGIPVAFHGHKCACGCTLISSFAAAKVA"
     gene            391..>1140
                     /locus_tag="ACHKIR_005335"
     CDS             391..>1140
                     /locus_tag="ACHKIR_005335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004170999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992909.1"
                     /translation="MPVWLDAIPEKAPKIARPNTRRWLLFLAFIMLGGMALTLWCWTS
                     ERTGFVFWFTALGLPFCTWGLIFGLRRFAYKAGQVGAESRNVEREALIDSEILRGQRC
                     AWILGTYIQSPAGNKADDLLEAMEVAAPAIDFSHPRGCDKPVRYAALPEYQTDLTKAL
                     KGAVNKLTTRVEGIVHPLPPALPCWLVLDCDNDLYPLIEEQLKADLSLKTGRIFRLMT
                     GKGLGAFDAWLDKRWDTPGILVVITLSLPASP"
BASE COUNT          277 a          269 c          312 g          282 t
ORIGIN      
        1 tcaataatca gtgacccgga aacattcata ataaaaatcc agacaatcac tatcgatggc
       61 agagcccagc ataaacatgg gaattaaaga gtgatgaatt agttaagaga taagaaagag
      121 gaatttaata atgcttcata ttattcgtaa aggcgataaa actactcacg gaggttccgt
      181 tctgaccgct tctgaaacga tgaaattcgg tgggataggc gtcgcccgta aaggcgataa
      241 agtgtcatgt ccgggtcatg gtcctaccat catcatagaa ggcaatcctg attatcttga
      301 ccatggtatc ccggtggcat ttcacggtca taaatgcgcc tgtggttgca cattgatcag
      361 ttcatttgcc gcagcaaagg ttgcctgatt atgcctgtct ggctggatgc catccctgaa
      421 aaggccccaa agatagcgcg ccccaatact cgccgctggc tgttgttcct ggcatttatc
      481 atgctggggg ggatggcgct cacattatgg tgctggacgt cagaacgaac aggatttgtc
      541 ttctggttca ccgctctggg gcttccattt tgcacgtggg ggcttatttt cggtttacgt
      601 cggtttgcct ataaagccgg gcaggtgggg gctgagtccc gaaatgtcga gcgggaagcg
      661 cttattgaca gtgaaattct gcgtggtcag cgctgtgcct ggatcctcgg tacttatatt
      721 cagagcccgg ccggaaacaa agccgatgac ctgcttgagg caatggaagt cgcggctcct
      781 gctattgatt tctcgcaccc aagaggatgt gataaaccgg tcaggtacgc ggcattacca
      841 gaataccaga cagatttaac gaaggctctg aaaggggcgg tgaataaact gaccacgcgt
      901 gtagagggga tagttcatcc gcttccgccc gcgctaccat gctggctggt gctggattgt
      961 gataacgatc tgtatcccct gattgaagag cagcttaaag ccgacctctc actcaagacg
     1021 ggcaggattt ttcgcctgat gaccggaaaa gggctggggg cgtttgatgc ctggctggac
     1081 aagcgatggg acaccccggg cattctggtt gtcatcacgc tgtcgttacc tgcctctccc
//
LOCUS       DAZGQL010000315         1138 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.319, whole genome shotgun sequence.
ACCESSION   DAZGQL010000315 DAZGQL010000000
VERSION     DAZGQL010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1138
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.319"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..686)
                     /locus_tag="ACHKIR_005336"
     CDS             complement(<1..686)
                     /locus_tag="ACHKIR_005336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HFO9992910.1"
                     /translation="MNRSLTSKAGSVVQQLSGQSRYRVHVHECSHFLDVLRYSAVESL
                     SQPWRYDVVVTCSSADIACDALLLKPASFTFQVPMSDGTPALPIRTVFGVVESFRRIF
                     ASNDETRYALTIVPRIALLNYTKGSEIYLNQSVTEVVEQVLRKHGLEGQDFEFRLSRE
                     YPPRELITRWRETDLEFIRRLLAEVGIYWRYEMDSRLEQDIVIFQDSQQQYEFGITLP
                     LRNQAGMSDSG"
     gene            complement(683..>1138)
                     /locus_tag="ACHKIR_005337"
     CDS             complement(683..>1138)
                     /locus_tag="ACHKIR_005337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000431644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HFO9992911.1"
                     /translation="SDLLMQQLSEKPETTESELHELIRPLLRDHFQPALLARFQTVIY
                     RPLTQSAMRTIVEMKLAQVCERLHCHYGLSTSVDERLYDALTSACLLPDTGARNVESL
                     LNQQLLPVLSRQLLSHMAAKQKPQALTLTWSDEDGIVMVFDAQAEGEPS"
BASE COUNT          260 a          334 c          301 g          243 t
ORIGIN      
        1 ccgctgtcac tcatcccggc ctgattgcgc aatggcagcg tgatgccaaa ttcatactgc
       61 tgctggctgt cctggaaaat cactatgtcc tgttcaagac ggctgtccat ttcatagcgc
      121 cagtaaatcc ccacctccgc cagcaggcgc cggataaact caaggtcagt ttcccgccac
      181 cgggtgatga gttccctagg tggatactcc cgggagagcc ggaattcaaa atcctgccct
      241 tccagaccat gtttacgtaa tacctgctca accacttcag tgactgactg attgaggtag
      301 atttcgctcc ctttcgtata gttcagaagg gcgatacggg gaacaatcgt cagtgcgtaa
      361 cgggtctcat cattcgaggc aaagatacga cgaaacgact caaccacgcc gaataccgtc
      421 ctgatcggca gggccggtgt tccgtcagac atcgggacct ggaaagtaaa tgacgccggc
      481 ttcagcagta acgcgtcaca ggcaatatcc gcagaagagc aggtgacaac cacgtcatag
      541 cgccagggct ggctgaggga ctcaaccgcg ctgtaacgca acacatccag aaaatggctg
      601 cattcatgca catgaacccg gtaccgtgat tgccctgaaa gctgctgtac aacgctgcca
      661 gccttgctgg tcagggaacg gttcatgacg gctctccctc tgcctgtgca tcaaatacca
      721 tcacaatacc gtcctcgtca ctccatgtca gagtcagcgc ctgtggtttc tgcttcgcgg
      781 ccatatggct cagcaactgc cggctcagta ccggcagcag ttgctggttc agcagactct
      841 ccacattccg ggcgcccgta tccggcagca ggcaggctga tgtcagggca tcgtacagac
      901 gttcatcaac cgatgtgctc agcccataat ggcagtgcag gcgttcgcag acctgtgcaa
      961 gcttcatctc cacaatggtg cgcatggcag actgcgtcag cggacggtaa atcacggtct
     1021 ggaaacgggc aagcagtgct ggctggaagt ggtcgcgcag cagtggacgg ataagctcat
     1081 gcagctccga ttccgttgtc tccggctttt cgctcagttg ctgcatcaga aggtcact
//
LOCUS       DAZGQL010000316         1137 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.320, whole genome shotgun sequence.
ACCESSION   DAZGQL010000316 DAZGQL010000000
VERSION     DAZGQL010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.320"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>1137
                     /locus_tag="ACHKIR_005338"
     CDS             <1..>1137
                     /locus_tag="ACHKIR_005338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFO9992912.1"
                     /translation="GLITRITTPDGRASAFYYNHHSQLTSATGPDGLELRREYDESGR
                     LIQETAPDGDITRYRYDNLHSDLPCATEDATGSRKTMTWSRYGQLLTVTDCSGYVTRY
                     DHDRFGQMTAAHREEGLSQYRAYDSRGQLIAVKDMQGHEMRYEYNAAGDLTAVIAPDG
                     SRNGTQYDTWGKAIRSTQGGLTRSMEYDAAGRVIRLTSENGSHTTFRYDVLDRLIQET
                     GFDGRTQRYHHDLTGKLIRSEDEGLVTHWHYDEADRITHRTVKGETAEQWRYDERGWL
                     TQISHLSEGHRVTVHYRYDEKGRLTGERQTVHHPQTEALLWQHETRHAYNAQGLANRC
                     IPDSLPAVEWLTYGSGWLAGMKLGDTPLVDFTRDRLHRETLRSFG"
BASE COUNT          276 a          336 c          339 g          186 t
ORIGIN      
        1 ggactcatca cgcgcatcac cacaccggat ggcagggcat cggcatttta ctataaccac
       61 cacagccagt taacgtcagc caccgggcct gacgggctgg aattgcgccg ggaatatgat
      121 gaatccggcc gtctgattca ggaaactgcc cctgacggcg atatcacccg ctaccgttat
      181 gataatctac acagtgactt accctgcgca acggaagatg ccaccggcag ccggaaaacc
      241 atgacatgga gccgttacgg tcagttgctg accgtcactg actgttccgg ttatgtaacc
      301 cgttatgacc atgaccgttt tggtcagatg acggcggcgc accgcgagga agggctgagt
      361 cagtatcgcg catacgacag ccgtggacag ttaattgcgg tgaaagacat gcagggccat
      421 gaaatgcggt atgaatacaa cgccgccggt gacctgaccg ccgtcattgc cccggacggc
      481 agcagaaacg ggacacagta cgatacgtgg ggaaaggcca tccgtagcac gcagggtggt
      541 ctgacgcgca gtatggaata cgatgctgcc ggacgggtca tccgcctgac cagtgaaaac
      601 ggcagccaca ccaccttccg ttacgatgta ctcgaccggc tgatacagga aaccggcttt
      661 gacggccgca cacagcgtta tcatcacgac ctgaccggta aacttatccg cagcgaggat
      721 gaggggctgg tcacccactg gcactatgac gaagcagacc gcatcacgca ccgcaccgtg
      781 aagggtgaaa ccgcagaaca gtggcggtac gacgaacgcg gctggctgac acaaatcagc
      841 catctcagcg aaggccaccg ggtgacggtg cattacaggt atgatgagaa aggccgcctg
      901 accggtgagc gccagacggt gcatcacccg cagacggaag cactgctctg gcagcatgag
      961 accagacatg cgtacaacgc gcaggggctg gcgaaccgct gtataccgga cagcctgccc
     1021 gccgtggaat ggctgactta cggcagcggc tggctggcag gcatgaaact cggcgacaca
     1081 ccgctggtgg atttcacccg tgaccgcctg caccgggaaa cgctgcgcag cttcggc
//
LOCUS       DAZGQL010000317         1137 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.321, whole genome shotgun sequence.
ACCESSION   DAZGQL010000317 DAZGQL010000000
VERSION     DAZGQL010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.321"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>1137
                     /locus_tag="ACHKIR_005339"
     CDS             <1..>1137
                     /locus_tag="ACHKIR_005339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFO9992913.1"
                     /translation="GLITRITTPDGRASAFYYNHHSQLTSATGTDGLELRREYDESGR
                     LIQETAPDGDITRYRYDNPHSDLPCATDDATGSRKTMTWSRYGQLLTVTDCSGYVTRY
                     DHDRFGQMTAAHREEGLSQYRAYDSRGQLIAVKDTQGHETRYEYNAAGDLTAVIAPDG
                     SRNGTQYDTWGKAIRTTQGGLTRSMEYDAAGRVIRLTSENGSHTTFRYDVLDRLIQET
                     GFDGRTQRYHHDLTGKLIRSEDEGLVTHWHYDEADRITHRTVKGETAEQWRYDERGWL
                     TGISHLSEGHRVTVHYRYDEKGRLTGERQTVHHPQTEALLWQHETRHAYNAQGLANRC
                     IPDSLPAVEWLTYGSGWLAGMKLGDTPLVDFTRDRLHRETLRSFG"
BASE COUNT          272 a          337 c          344 g          184 t
ORIGIN      
        1 ggactcatca cgcgcatcac cacgccggat ggcagggcat cggcgtttta ctataaccac
       61 cacagccagt taacgtcagc caccgggact gacgggctgg agttgcgcag ggaatatgat
      121 gaatccggcc gtctgattca ggaaactgcc cctgacggcg atatcacccg ctaccgttat
      181 gataatccac acagtgactt accctgcgca acggacgatg ccaccggcag ccggaaaacc
      241 atgacatgga gccgttacgg tcagttgctg accgtcactg actgttccgg ttatgtaacc
      301 cgttatgacc atgaccgttt tggtcagatg acggcggcgc accgcgagga agggctgagt
      361 cagtatcgcg catacgacag ccgtggacag ttaattgcgg tgaaagacac gcagggccat
      421 gaaacgcggt atgaatacaa cgccgccggt gacctgaccg ccgtcattgc cccggacggc
      481 agcagaaacg ggacacagta cgatacgtgg ggaaaggcca tccgtaccac gcagggtggt
      541 ctgacgcgca gtatggaata cgatgctgcc ggacgggtca tccgcctgac cagtgaaaac
      601 ggcagccaca ccaccttccg ttacgatgta ctcgaccggc tgatacagga aaccggtttt
      661 gacggccgca cacagcgtta tcatcacgac ctgaccggta aacttatccg cagcgaggat
      721 gaggggctgg tcacccactg gcactatgac gaagcagacc gcatcacgca ccgcaccgtg
      781 aagggtgaaa ccgcagaaca gtggcggtac gacgaacgcg gctggctgac aggcatcagc
      841 catctcagcg aagggcaccg ggtgacggtg cattacaggt atgatgagaa aggccgcctg
      901 accggtgagc gccagacggt gcatcacccg cagacggaag cactgctctg gcagcatgag
      961 accagacatg cgtacaacgc gcaggggctg gcgaaccgct gtataccgga cagcctgccc
     1021 gccgtggaat ggctgactta cggcagcggc tggctggcag gcatgaaact cggcgacaca
     1081 ccgctggtgg atttcacccg tgaccgcctg caccgggaaa cgctgcgcag cttcggc
//
LOCUS       DAZGQL010000318         1123 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.322, whole genome shotgun sequence.
ACCESSION   DAZGQL010000318 DAZGQL010000000
VERSION     DAZGQL010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1123
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.322"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(124..264)
                     /gene="yjjY"
                     /locus_tag="ACHKIR_005340"
     CDS             complement(124..264)
                     /gene="yjjY"
                     /locus_tag="ACHKIR_005340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjjY"
                     /protein_id="HFO9992914.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            360..1076
                     /gene="arcA"
                     /locus_tag="ACHKIR_005341"
     CDS             360..1076
                     /gene="arcA"
                     /locus_tag="ACHKIR_005341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HFO9992915.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
BASE COUNT          294 a          259 c          279 g          291 t
ORIGIN      
        1 ataatgtgac gaaagctagc atttagatac gatgatttca tcaaactgtt aacgtgctac
       61 aattgaactt gatatatgtc aacgaagcgt agttttattg ggtgtccggc ccctcttagc
      121 ctgttatgtt gctgttaaaa tggttaggat gacagccgtt tttgacactg tcgggtcctg
      181 agggaaagta cccacgacca agctaatgat gttgttgacg ttgatggaaa gtgcatcaag
      241 aacgcaatta cgtactttag tcatgttacg ccgatcatgt taatttgcag catgcatcag
      301 gcaggtcagg gacttttgta cttcctgttt cgatttagtt ggcaatttag gtagcaaaca
      361 tgcagacccc gcacattctt atcgttgaag acgagttggt aacacgcaac acgttgaaaa
      421 gtattttcga agcggaaggc tatgatgttt tcgaagcgac agatggcgcg gaaatgcatc
      481 agatcctctc tgaatatgac atcaacctgg tgatcatgga tatcaatctg ccgggtaaga
      541 acggtcttct gttagcgcgt gaactgcgcg agcaggcgaa tgttgcgttg atgttcctga
      601 ctggccgtga caacgaagtc gataaaattc tcggcctcga aatcggtgca gatgactaca
      661 tcaccaaacc gttcaacccg cgtgaactga ctattcgtgc acgcaacctg ctgtcccgta
      721 ccatgaatct gggtactgtc agcgaagaac gtcgtagcgt tgaaagctac aagttcaatg
      781 gttgggaact ggacatcaac agccgttcgt tgatcggccc tgatggcgag cagtacaagc
      841 tgccgcgcag cgagttccgc gccatgcttc acttctgtga aaacccaggc aaaattcagt
      901 cccgtgctga actgctgaag aaaatgaccg gccgtgagct gaaaccgcac gaccgtactg
      961 tagacgtgac gatccgccgt attcgtaaac atttcgaatc tacgccggat acgccggaaa
     1021 tcatcgccac cattcacggt gaaggttatc gcttctgcgg tgatctggaa gattaatcgg
     1081 ctttaccacc gtcaaaaaaa cggcgctttt tagcgccgtt ttt
//
LOCUS       DAZGQL010000319         1115 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.323, whole genome shotgun sequence.
ACCESSION   DAZGQL010000319 DAZGQL010000000
VERSION     DAZGQL010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1115
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.323"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            7..>1115
                     /locus_tag="ACHKIR_005342"
     CDS             7..>1115
                     /locus_tag="ACHKIR_005342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014728563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HFO9992916.1"
                     /translation="MEMPDLPETPTSDIPTGANNQPWDCTTTGVEMVEKQSRLPTTEN
                     RQYLHIKVEYAMRFPQLSAQIRRKGKTYQMSLKQTVMSGLIRVDEIARNYCWYYKAEV
                     AFDMRTTPPRPFLSSTLLEKDGAGRRHTTNPFPVPGRGIYRRPDVIIVKNKEDRWPGL
                     AGPDTEGNMHSDNLARLVEVKFPGDRLYEDQYEEYMQIVGGIGHEDRMTILEIHDCRP
                     EEHQWVDQVINVTMKAWQTSGAKYWPLALYPPVFMPRPPSTPVAARIEPWTYAGKAVS
                     DLSEGVVRGVAEGWNALSKETQLVLNSAASWLNDSGEWVYRQGSEVWVWASQTGKSVK
                     SWTNTQIKAAWTEIQHVTDMTLDMLKQVDWVQVLT"
BASE COUNT          280 a          286 c          325 g          224 t
ORIGIN      
        1 gccataatgg aaatgcccga tttacccgag acaccgacat ctgatattcc caccggggcg
       61 aacaaccagc cgtgggactg cactacgacg ggtgtcgaaa tggtcgaaaa gcagtcgcgt
      121 ctgccaacca cggaaaaccg tcagtacctg catataaagg tggaatacgc catgcgtttt
      181 ccgcaactgt cggcacaaat ccgccggaaa ggtaaaactt accagatgag ccttaagcag
      241 accgttatga gtggtcttat tcgtgtggat gaaatcgccc gcaattactg ctggtattat
      301 aaggcggagg tggcgtttga tatgcgcacc acgccaccac gcccttttct gagcagtacg
      361 ctgctggaaa aagacggtgc tggccgtcgc cacacgacca atcctttccc tgtgcccggc
      421 aggggcattt accgccgccc ggacgtgatt attgttaaaa ataaggagga ccgctggccc
      481 ggactggcgg ggccggatac cgaaggcaat atgcacagcg ataacctggc ccgcctggtg
      541 gaggtgaagt ttccgggaga taggctgtat gaagaccaat atgaagagta tatgcagatt
      601 gtcggtggaa ttggtcatga ggatcgcatg accattctgg agatccacga ctgtcgcccg
      661 gaagagcacc agtgggttga tcaggtgatc aacgtgacga tgaaagcctg gcagaccagc
      721 ggcgcgaaat actggccgct ggctctctac ccgccggtct ttatgccccg tccgccatca
      781 acccccgttg ctgccagaat agagccctgg acttacgccg gtaaagcagt atctgatctc
      841 agtgagggtg tggtacgtgg tgtggctgaa ggctggaatg ccctgtcaaa agagacgcaa
      901 ctggtcctga attcagcagc cagctggctt aacgacagcg gtgagtgggt ttaccggcag
      961 ggaagtgagg tctgggtctg ggccagccaa accggtaagt cggtgaaatc ctggaccaac
     1021 acccagatta aggccgcatg gacagaaata cagcacgtca ccgatatgac gcttgatatg
     1081 ctgaaacagg tggactgggt acaggtttta accaa
//
LOCUS       DAZGQL010000320         1115 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.324, whole genome shotgun sequence.
ACCESSION   DAZGQL010000320 DAZGQL010000000
VERSION     DAZGQL010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1115
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.324"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..642
                     /locus_tag="ACHKIR_005343"
     CDS             <1..642
                     /locus_tag="ACHKIR_005343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001178557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFO9992917.1"
                     /translation="GFGKTGEKFPADAFILNPPYSAKGNGMIFVQKALSMMDKGYAAV
                     IIQSSAGTGKATEYNKKILKENTLLASIKMPADLFIGKSSVQTYIYVFQVKIPHNAKQ
                     AVKFIDFSNDGYARSNRKKARNNLVDADRAKERYQEVVDLVHFGKGCLNIFTEDEYFE
                     GTIDPDSGEDWNQTRPVDARPTLDDFKKTVGDYLAWEVSQLLKKQGENNFAGK"
     gene            684..1115
                     /locus_tag="ACHKIR_005344"
                     /pseudo
     CDS             684..1115
                     /locus_tag="ACHKIR_005344"
                     /inference="COORDINATES: protein motif:HMM:NF013579.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
BASE COUNT          350 a          189 c          268 g          308 t
ORIGIN      
        1 ggatttggga aaacaggaga aaaattccct gcggacgcat ttattctcaa tcctccgtat
       61 tcagctaaag gtaacggcat gattttcgtg cagaaggcgc tgtcgatgat ggataaaggc
      121 tatgctgcag tgattatcca gagttcagcc ggcacaggaa aggctacgga gtacaacaaa
      181 aaaatactca aggaaaatac cttgctggca agcatcaaaa tgcctgcgga tctgtttatt
      241 ggtaaatcaa gcgttcagac gtacatttac gtttttcagg taaaaattcc gcataacgcg
      301 aaacaggctg ttaagtttat cgacttttcc aatgacggct atgctcgctc taatcgcaaa
      361 aaggcaagaa acaatcttgt tgatgccgat cgtgcaaaag agcgttatca ggaggttgtg
      421 gatctggttc acttcgggaa aggttgcctt aatatcttta cggaggatga atattttgag
      481 ggaacaatcg atcctgatag tggtgaggac tggaatcaga ccaggccagt agatgctagg
      541 ccaacgctgg atgatttcaa aaagacggta ggtgattacc ttgcatggga ggtctcgcaa
      601 ttactgaaaa aacaaggtga aaacaatttt gcgggaaagt aaattccccg ctcagcgata
      661 aattaagaag cgttgagtgg ggagagttca ggattggcga tttgttctat gtgagcacag
      721 gctcgcttat ctcctcagga agattaaaac caggcaaaat taaacgtgtt tcagcaaaga
      781 gcgatcaaaa cggtatcatc ggagagtttg ataccgagtt tatggatgat gcaaggcatt
      841 atgaaaattt tataagcgtt aatttttttg gagatgcttt ttatcatcct tattgtgcca
      901 gcgtagaaat gaaggtgcat gttcttacat taaaatcaag taagtccgac tttaatgcaa
      961 agcgcggact gtatgttgcc agtatgatta ataaagcttt aaaggggcgt tttaattatg
     1021 gtcaacagtt atcgagttcc aaactcagag atggagaata tttcatctca ttaccagtga
     1081 aaaataatga aattgactgg ttttttatgg ataat
//
LOCUS       DAZGQL010000321         1114 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.325, whole genome shotgun sequence.
ACCESSION   DAZGQL010000321 DAZGQL010000000
VERSION     DAZGQL010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1114
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.325"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>1114
                     /locus_tag="ACHKIR_005345"
     CDS             <1..>1114
                     /locus_tag="ACHKIR_005345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HFO9992918.1"
                     /translation="FDAYDAILKKYPGDIPVIMAEQGLLGLKNISEVLINSYVSLDFD
                     SSGDKINKLSKFKQVELFKEDLSLEDLIESPEISGVDSGDKNIEEQSESELAPEQKRT
                     GIAKMLMTTPVLTLIFDRVRYDEIELQPDFQRKDRIWPDDKKSKLIESILMKLPLPGF
                     YFGEKPNGNWVVIDGLQRTTTICDYMSGHFSLKGLSILEHLNGKSFKDLTRTEQRDIR
                     EYQITAYQIELNDDSSELVVELFHRINTYGVKLSSQEIRSALNKGNSVTFLRYLASLE
                     TFKKATQFKVKPDRQKDMELCLSALAFMVLGYNNYGQHSYDHFLCSAMQKLNNYPLSI
                     INKEEIDAGTALISPSSEVFLTLYSKYNQALILANEV"
BASE COUNT          389 a          160 c          217 g          348 t
ORIGIN      
        1 tgttcgatgc gtatgatgct attttaaaaa aatacccagg ggatatccct gttataatgg
       61 ctgagcaagg cttgttggga ttaaaaaaca ttagcgaagt actgattaat tcttatgttt
      121 ccttggattt tgacagtagt ggcgataaaa taaataaatt aagtaaattt aaacaggtgg
      181 agttatttaa agaagattta tctttagagg atttaattga gtcccctgag ataagtggag
      241 ttgattcagg tgataagaat attgaggaac aatcggaatc agaattagca cctgagcaaa
      301 aacgtacggg cattgcaaaa atgcttatga ctacaccagt tttaacacta atttttgatc
      361 gtgtaagata tgatgaaatt gagttacaac ctgactttca gagaaaagac cgaatttggc
      421 ctgacgacaa aaagtcaaaa cttattgaat caatattaat gaagcttcca ttaccggggt
      481 tttactttgg tgaaaagcca aacggtaact gggtagttat tgatggcctt caaagaacga
      541 ctacaatttg tgattatatg agtggtcatt tctcattgaa agggctctct attttagagc
      601 acttaaatgg aaagagtttt aaagatctta ctcgaaccga gcaacgagac attagagagt
      661 atcaaattac ggcttaccaa atagaactaa atgatgatag ttctgaattg gttgtagaac
      721 tattccatag aataaacact tatggtgtaa agttaagtag tcaagaaata agatctgcac
      781 ttaataaagg caatagcgta acctttttac gttatttagc ttcattagaa acgttcaaaa
      841 aagcgacgca atttaaagta aagccggaca ggcaaaaaga tatggagctc tgtttgtccg
      901 ctcttgcttt tatggtactt ggttataata actatggcca acattcgtat gatcattttt
      961 tatgtagtgc aatgcagaag ctaaataatt atccattaag catcattaat aaagaagaga
     1021 ttgatgctgg aacagctctt atttctcctt caagtgaagt ctttttaact ttatattcaa
     1081 aatataatca ggctttaatc ttagcaaatg aagt
//
LOCUS       DAZGQL010000322         1095 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.326, whole genome shotgun sequence.
ACCESSION   DAZGQL010000322 DAZGQL010000000
VERSION     DAZGQL010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1095
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.326"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            209..>1095
                     /locus_tag="ACHKIR_005346"
     CDS             209..>1095
                     /locus_tag="ACHKIR_005346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001379687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HFO9992919.1"
                     /translation="MEARAEATSARSRVEQARSAHWPVVTATGSKLLSQSHRYDYDYD
                     SEDILPGIRGEMNLFASGAIEADVSRSESEAEYYRYKVDEAGEETIRSFVSLYLDALR
                     EKQSIAVLEQSLSRHNAILNDLNTISIHDTGRESEFVQAEARRLMVRQQINTRTRVLK
                     TTLGKLSTWTTNPVTEADLENPFSRMTESRLLTDFTQTPQKGNPSWLASQADVESKKA
                     ALKAQELARYPRVDLTGSVTRDDRQIGVSLSWDLFNRNASYGVSEKAAQIAAATGRLD
                     SVARMIDETGRLSLITVRQ"
BASE COUNT          264 a          272 c          285 g          274 t
ORIGIN      
        1 gcttggatca attccggtgt ctctgcattt caattttact cgatatttta cccggttgca
       61 tcatgatgcc tgattatatt tcccgcgtgt cccggaggtt cgggtgaaga gaatattatc
      121 tttctgtttt cttttctctg tccacaacgt gttcgcttct ccactgccgg ccctcctgag
      181 ggatgctctc attcatgatc catcagtgat ggaggcaagg gcagaagcca cctctgcccg
      241 ttcccgtgtg gagcaggctc gttcagcaca ctggccggtc gtcacagcaa caggcagtaa
      301 actcctttcc cagagtcacc gctacgacta tgactacgac tctgaagata ttttgcccgg
      361 tatccgcggt gaaatgaatc tgtttgcttc gggagctatt gaggcggatg tcagccggag
      421 tgagtcggaa gccgagtatt accgttataa agttgatgag gctggcgagg aaacaattcg
      481 ctcctttgtt tcattatatc ttgatgcgct aagggaaaag caatccattg cggtacttga
      541 acagagtctc tcccggcata acgcaattct taatgatctt aacaccatca gtattcacga
      601 taccgggcgg gaatctgaat ttgttcaggc cgaagcccgc aggctgatgg tccggcagca
      661 gataaatacc cgcacccgag tgctcaaaac cacgctggga aaactttcga cctggaccac
      721 aaatccggta accgaagccg atcttgaaaa tcctttttcc cggatgacag agtccagatt
      781 gttaactgat tttacgcaga cgcctcagaa agggaatccg tcgtggcttg ccagtcaggc
      841 tgatgtagag agtaaaaaag cggcgctgaa agcgcaggag cttgcccggt atccccgggt
      901 ggatttaacg ggatcagtga cccgggatga caggcagata ggtgtcagtc tgtcctggga
      961 cctgttcaac cgtaatgcca gttatggtgt cagtgaaaag gccgcgcaga tagctgcagc
     1021 taccggacga ctggactctg ttgcccgaat gattgatgaa accggacgat tatcgctgat
     1081 aacggtcaga caaag
//
LOCUS       DAZGQL010000323         1046 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.327, whole genome shotgun sequence.
ACCESSION   DAZGQL010000323 DAZGQL010000000
VERSION     DAZGQL010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1046
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.327"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            51..356
                     /locus_tag="ACHKIR_005347"
     CDS             51..356
                     /locus_tag="ACHKIR_005347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001604127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992920.1"
                     /translation="MNPLFNDIQMRLFYLNHAPYSWHWNVRFRPQEAVYIGNDACHIT
                     ITCNQSGFHLTRDGQRLFTERYIRTLSELLAVLKRRWDVTPAIIRAVEYLSRVPVSH"
     gene            411..932
                     /locus_tag="ACHKIR_005348"
                     /pseudo
     CDS             411..932
                     /locus_tag="ACHKIR_005348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200223.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ImpA family N-terminal
                     domain-containing protein"
BASE COUNT          261 a          262 c          276 g          247 t
ORIGIN      
        1 tatggttccc cttatccgtg tgcttaacag acataaacga gggctgaatg atgaacccgt
       61 tatttaatga catccagatg cgcctttttt atctgaatca tgcaccgtat tcctggcact
      121 ggaatgtcag attcaggcca caggaagccg tatatattgg taatgatgcc tgccatatta
      181 ccattacctg taatcagtcc ggttttcatt taaccaggga cgggcagcga ctgttcactg
      241 aacgttatat cagaacactc agtgagttat tggcggttct gaagcggcgg tgggatgtga
      301 caccggcaat catccgtgcg gtggaatacc tttcccgggt accggtatca cactgacgct
      361 ctttccggga atattaatta tgatttgata cagcaacact gaatctgccg atgacttctg
      421 aagtaaaact gaaaacaggt aatgaccccc ggtcattacc ggattatgct gccctgcgcg
      481 atgaaatcag caagctgact cacccggcac gtccggatgt ggactggcgt tatgtggaga
      541 cgctctgcct gcgtctgtat gaacacaatg gcgtggaatt acagacggca tcctggtaca
      601 ccatcgcccg gatgcataca acagggttaa gtggcctgaa tgaagggctg gctctgattg
      661 tggcgctgac ccggcatcac tggccagtga tgtggccgct gaatacccac gcccggctgg
      721 aaattattac cggtctgttc agtcgtctgc aaaaaacgct ccgggccatg ccaccggatg
      781 agcgtgacaa cctgcccctg ctgtatcaga cagaaacatg cctgaaaacg ctgagtgaca
      841 tactgacccg gcatgaactt aaacagagca gtaaagtggc tttaccggaa gccatggtga
      901 agggatatat cacccgtctg gagaatcagc cgtaagcgta cagcgagggc catattgacg
      961 gggatgtgtt attcagctgg cagtgctatg cgccacggaa gcagttcgct gacccggttg
     1021 accggccagt ctgctatgac gccaag
//
LOCUS       DAZGQL010000324         1027 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.328, whole genome shotgun sequence.
ACCESSION   DAZGQL010000324 DAZGQL010000000
VERSION     DAZGQL010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1027
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.328"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>1027
                     /locus_tag="ACHKIR_005349"
     CDS             <1..>1027
                     /locus_tag="ACHKIR_005349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RTX toxin"
                     /protein_id="HFO9992921.1"
                     /translation="VFNVVGENGQVTEAVLTQDGPVLENFTAGQSGTLSDDDRGGFIW
                     LGGLAFGGGLAAMALAAGGGGGSKHRHENHEADTTAPSAPELTAGDNGSVSVELPGDA
                     NKGDTVEITFEDENGDEQKVTLEKGDNGWTSSNPALIPDSQGDTATIAPDTVKDNSEV
                     TAVAKDPAGNESTPVTVTSKTDGVADAPVLTIPEVADSVANAAELKDGLQAEVKLPAG
                     TVEGAVITLTVTHPDQSTENVTHNVTGDEVTAGKVSMDIPEDAVVDGQNSVSVSLTQG
                     SNPAKAGNKVEFVVDGQVPGDTNGDGVADVTPAVAIPEATDGVNAKELKDGVQAEVTV
                     PAGSAEGD"
BASE COUNT          274 a          206 c          325 g          222 t
ORIGIN      
        1 gtatttaatg ttgtgggtga aaatggtcag gtaactgaag ctgtcctgac ccaggatggc
       61 cctgtactgg agaatttcac tgcaggacaa tccgggacct tgtcagatga tgatcgtggc
      121 ggttttatat ggttgggcgg tctggcattt ggtggggggc ttgctgctat ggctttggct
      181 gcaggtggtg gtggcggcag caagcacaga catgaaaacc atgaggcaga tacaacagca
      241 ccgtcagcac cggaattaac ggccggagat aatggttcag tttctgttga actgccgggt
      301 gatgcaaata aaggcgatac tgttgaaatc acctttgaag atgaaaatgg tgatgaacag
      361 aaagtcacac tggagaaagg ggataacggc tggacatcca gcaatcctgc actgatcccg
      421 gacagccagg gtgatacagc cacaatcgcc ccagacactg ttaaagataa cagtgaagtc
      481 acagcagttg ctaaagatcc ggctggtaat gaaagtactc cggtcactgt gacgtcaaaa
      541 actgatggtg tggcagatgc tccggtcctg accattccag aagtggctga tagtgtggcc
      601 aatgcggctg agctgaaaga tggtttacag gcagaagtca agttgccggc aggaacagtg
      661 gaaggtgcgg tcattacact gacagtgact catccggatc aaagtaccga aaatgtgaca
      721 cataacgtca ccggagatga ggttactgcc ggtaaggtat ccatggatat tcctgaggat
      781 gctgttgttg acggtcagaa cagtgtgagt gtcagcctga cccagggcag taatccggct
      841 aaagcgggga ataaagtgga gtttgtggtt gacgggcagg ttccgggtga caccaatggt
      901 gatggtgtgg cggatgtcac accggcagtg gccattccgg aagcgacgga cggtgtgaat
      961 gccaaagaac tgaaggatgg cgtacaggca gaagtgacgg ttccggcagg cagtgctgaa
     1021 ggtgata
//
LOCUS       DAZGQL010000325         1020 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.329, whole genome shotgun sequence.
ACCESSION   DAZGQL010000325 DAZGQL010000000
VERSION     DAZGQL010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.329"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..268)
                     /locus_tag="ACHKIR_005350"
     CDS             complement(<1..268)
                     /locus_tag="ACHKIR_005350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001210963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HFO9992922.1"
                     /translation="MRFICDNSYFLEGVKEYINPGKFIISGMQTDNVVIISSSRISEI
                     VGFLYKNNISDMNTLYFSSPEALRFLWGVVDSPVYDIRHLTERCS"
     gene            complement(392..868)
                     /locus_tag="ACHKIR_005351"
     CDS             complement(392..868)
                     /locus_tag="ACHKIR_005351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001165712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HFO9992923.1"
                     /translation="MQIIIMTRDRYLEYGLMRMLNGYRLTTGRELFDAGKRRLPLPED
                     SYVILCDRNLERLTYCMFCGRRFLVIPVSSVRCLTDIRQAIRRGAWLFGHTARPLTRT
                     EMVVVFGVIFHEYGCTFLADQLGISMKTVCAHLYNAMEKSGLRGVSVKYLCSTADR"
BASE COUNT          289 a          292 c          195 g          244 t
ORIGIN      
        1 ctgaacatcg ctcagtaaga tgtctgatat cataaaccgg actgtcaacc actccccaga
       61 gaaaacggag cgcttccgga gaagaaaaat aaagggtgtt catatcactt atattattct
      121 tatacagaaa gccgacaatt tcagatatcc gggaagaaga tattatcacc acattatctg
      181 tctgcatgcc gctgataatg aatttaccgg gattaatata ctccttcact ccctccagaa
      241 aataactgtt atcacaaata aaacgcatac ccgttccaca tgaaatttat cacgcctgac
      301 cacataacat taattatccg gagcaccggg atgcccgtga cacagcatgc acagaaattc
      361 ccgtcgcaac agccttcatc agaccggctt tttaccggtc tgcggtgctg cagagatatt
      421 taacactgac acctcgcaga ccgcttttct ccatcgcatt gtaaagatgc gcacataccg
      481 tcttcatgct tatccccagc tggtctgcca gaaaggtaca gccgtactca tggaaaataa
      541 ccccgaagac caccaccatc tcggtacggg tcagtggtct tgccgtatgt ccgaacagcc
      601 acgcaccacg acggatggcc tgcctgatat ctgtcaggca tctcacagag gaaacaggaa
      661 tgacaagaaa acgacgccca cagaacatac agtatgtaag cctttccaga ttacggtcac
      721 agagaatcac ataactgtct tcgggaagtg gcagacggcg ctttccggca tcaaacagtt
      781 cacggcctgt cgtgagcctg tatccgttca gcatacgcat caggccatat tccagatatc
      841 tgtcacgggt cataataata atctgcatac tctcagaccc ttatctcccg gataccggct
      901 ccgttcacca ttaccggtat aactgactca tcattcataa cggaaactgt ctgccgcata
      961 catcagtgcc cgctcttcca gctctgacag actcagctct ttcagttccc cttctccttc
//
LOCUS       DAZGQL010000326         1005 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.330, whole genome shotgun sequence.
ACCESSION   DAZGQL010000326 DAZGQL010000000
VERSION     DAZGQL010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1005
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.330"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>1005
                     /locus_tag="ACHKIR_005352"
     CDS             <1..>1005
                     /locus_tag="ACHKIR_005352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS domain-containing protein"
                     /protein_id="HFO9992924.1"
                     /translation="TSAGQLQSQHLNSLQYDRDYTWNDNGELIRISSPRQTRSYSYSD
                     SGRLTGVHTTTSNLDIRIPYATDPAGNRLPDPELHPDSTLSMWPDNRIARDAHYLYRY
                     DRHGRLTEKTDLIPEGVIRTDDERTHRYHYDSRHRLVHYTRTQYAEPLVESRYLYDPL
                     GRRVAKRVWRRERDLTGWMSLSRKPEVTWYGWDGDRLTTIQNDRTRIQTVYQPGSFTP
                     LIRVETATGELAKTQRRSLADALQQSGGEDGGSVVFPPVLVQMLDRLESEILADRVSE
                     ESRRWLASCGLTVEQIQNQMDPVYTPARKIHLYHCDHRGLPLALVSTEGATEWCAEYD
                     "
BASE COUNT          230 a          317 c          311 g          147 t
ORIGIN      
        1 atacctctgc cgggcagtta cagagccagc acctgaacag cctgcagtat gaccgcgatt
       61 acacctggaa cgacaacggc gaactcatcc gcatcagcag cccgcgacag acccggagtt
      121 acagctacag cgactccggc aggctgaccg gcgtccacac caccacctca aatctggata
      181 tccgcatccc gtatgccacg gacccggcag gtaaccgcct gcccgacccg gagctgcacc
      241 cggacagcac cctcagcatg tggccggata accgtatcgc ccgtgacgcg cactatcttt
      301 accggtatga ccgtcacggc aggctgacag agaaaaccga cctcatcccg gaaggggtta
      361 tccgcacgga tgatgagcgg actcaccggt accattacga cagccggcac cggctggtgc
      421 actacacgcg gacacaatat gcagagccgc tggtcgaaag tcgctatctt tacgacccgc
      481 tgggccgcag ggtggcaaaa cgggtatggc ggcgtgaacg ggacctgacg ggctggatgt
      541 cgctgtcacg gaaaccggaa gtgacctggt acggctggga cggcgaccgg ctgaccacga
      601 tacagaacga cagaacccgc atccagacgg tttatcagcc ggggagcttc acgccactca
      661 tcagggtcga aaccgccacc ggagagctgg cgaaaacgca gcgccgcagc ctggcggatg
      721 cccttcagca gtccggcggc gaagacggtg gcagtgtggt gttcccgccg gtgctggtgc
      781 agatgctcga ccggctggaa agtgaaatcc tggctgaccg ggtgagtgag gaaagccgcc
      841 gctggctggc atcgtgcggc ctgacggtgg agcagataca aaaccagatg gacccggtgt
      901 acacgccggc gcgaaaaatc cacctgtacc actgcgacca tcgcggcctg ccgctggcgc
      961 ttgtcagcac ggaaggggca acagaatggt gcgcagaata cgatg
//
LOCUS       DAZGQL010000327         1001 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.331, whole genome shotgun sequence.
ACCESSION   DAZGQL010000327 DAZGQL010000000
VERSION     DAZGQL010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.331"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..353)
                     /gene="tssK"
                     /locus_tag="ACHKIR_005353"
     CDS             complement(<1..353)
                     /gene="tssK"
                     /locus_tag="ACHKIR_005353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004171008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HFO9992925.1"
                     /translation="MKIYRPLWSEGVLLSPQQFQQQTEWESFRSAGISALASPFPWGV
                     EKVELNDSLLSSGLIQITQLRLWLEDGTLIDAQRSDLPPAPRELDAGQLADHDAVTVV
                     IALPHMQPGVINVEQ"
     gene            complement(369..>1001)
                     /gene="tssC"
                     /locus_tag="ACHKIR_005354"
     CDS             complement(369..>1001)
                     /gene="tssC"
                     /locus_tag="ACHKIR_005354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HFO9992926.1"
                     /translation="FAANMVRSFVTNGWCVQIRGPQAGGAVQDLPIHLYDLGTGNQVK
                     IPSEVMIPETREFEFANLGFIPLSYYKNRDYACFFSANSTQKPALYDTPDATANSRIN
                     ARLPYIFLLSRIAHYLKMIQRENIGTTKDRRLLELELNNWIRGLVTEMTDPGDELQAS
                     HPLRDGKVVVEDIEDNPGFFRVKLYAVPHFQVEGMDVSLSLVSQMPKAKA"
BASE COUNT          250 a          286 c          250 g          215 t
ORIGIN      
        1 tcctgctcaa cattaatcac gccaggctgc atgtggggaa gtgcaataac gacagttacc
       61 gcatcgtggt cagccagctg accggcatct aactctcgcg gtgccggagg caaatcgctc
      121 ctttgagcat caataagtgt tccgtcttcc agccagaggc gtaactgcgt aatctggatc
      181 agcccggatg acagaagact gtcattaagc tcaacctttt ccactcccca gggaaacggg
      241 gatgccagcg cagaaatacc ggcactgcga aaagactccc attcagtctg ctgctgaaac
      301 tgctgagggg agagcaaaac accttcgctc cacagcggac ggtaaatctt catttcccga
      361 cgcctgcctt acgcttttgc cttcggcatc tgcgaaacca gggacagact gacatccata
      421 ccttccacct ggaaatgcgg tacagcgtag agtttcacac ggaaaaatcc cgggttgtct
      481 tcgatatctt ccaccaccac ttttccgtca cgcagcggat gcgaggcctg caactcatca
      541 cccggatcgg tcatctccgt caccagaccc cggatccagt tattcagctc cagctcaagc
      601 agacggcgat ctttggtggt accgatattt tcccgctgaa tcatcttcag gtaatgcgca
      661 atgcgggaca gcaggaagat atagggaaga cgggcattaa tacggctgtt cgccgttgca
      721 tccggcgtat cgtacagtgc cggtttctgg gtggaattag cggagaagaa gcaggcataa
      781 tcccggttct tgtagtaaga gagcggaata aagcccagat tagcgaattc aaactcccgc
      841 gtttcaggga tcatcacctc tgacggaatt ttgacctgat taccggttcc cagatcatac
      901 agatggatcg gcaaatcctg caccgcaccg ccggcctgag gaccgcggat ctgaacacac
      961 cagccgttgg tgacaaaact gcgcaccata ttggccgcaa a
//
LOCUS       DAZGQL010000328          998 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.332, whole genome shotgun sequence.
ACCESSION   DAZGQL010000328 DAZGQL010000000
VERSION     DAZGQL010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..998
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.332"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(123..>998)
                     /locus_tag="ACHKIR_005355"
     CDS             complement(123..>998)
                     /locus_tag="ACHKIR_005355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFO9992927.1"
                     /translation="VPTTPWLNTAPGLFIDGYTSAAASTVPSLSRTLIYDYEQNPDSG
                     NNVVALAAKAGYSTWWISNQGKLGEHDTRISVIASDAEHTVFLKKGSFASRKTDDMLL
                     LQETERALADKSSPKVIFLHMIGSHPNPCDRLNSWPNHYLEQYPRKIACYLASISKLD
                     NFLGQLDGILRRHSRHFAMLYFSDHGLSVSDSANPVHHDGHVQGGYSVPLIITASDIT
                     SHQSVSRKISARHFAGIFQWLTGIRTENIPPFNPLVDREPGPVMVFNGEKNVPADSLK
                     SQRLIYPDSNNHTGK"
BASE COUNT          266 a          237 c          240 g          255 t
ORIGIN      
        1 ctcatcaaat agctgtaatc cggtcagatt atttctctcc cacattgttg tacaactcaa
       61 ctttaataaa attattataa aaagcaggaa tatatcattt accagtcatc gtcagatacc
      121 attcacttac ctgtatgatt attactgtca ggataaataa gtctctgtga cttaaggctg
      181 tctgcaggaa catttttctc gccattaaac accataaccg gccccggctc tctgtccacg
      241 agcgggttga atggcggtat attttcagta cgaatgccgg tcagccactg aaaaatacct
      301 gcgaaatgac gggcactgat tttcctgctg acggactgat gagacgtgat gtcactggcg
      361 gtaataatca ggggaacgct gtagcctccc tgcacatgac catcatgatg aacaggattg
      421 gcactatcgc tgaccgacag cccatggtca gaaaagtaaa gcatggcaaa atgacgggaa
      481 tgccggcgaa ggataccatc aagctgaccg agaaagttat ccagtttact gatgctggcg
      541 aggtaacagg caatctttcg gggatactgc tccaggtaat gattcggcca ggagttaagt
      601 cggtcacacg gattcggatg agagccaatc atgtgcagga aaatcacctt cggagaggat
      661 ttatccgcca gcgcacgttc tgtttcctgt aacaacaaca tgtcatccgt tttacgggaa
      721 gcgaagctgc ctttcttgag gaaaacggta tgctccgcat cagaagcaat aacagagatg
      781 cgtgtgtcat gctcccccag ttttccctga ttggatatcc accatgtgct gtatcctgct
      841 tttgctgcca gcgccaccac gttgttgccg gagtcagggt tctgctcata gtcataaatc
      901 agtgtccggc tcagggaagg tacggtactg gctgctgccg atgtatagcc gtcaataaat
      961 aaaccgggag ccgtattcag ccacggtgtg gttggtac
//
LOCUS       DAZGQL010000329          996 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.333, whole genome shotgun sequence.
ACCESSION   DAZGQL010000329 DAZGQL010000000
VERSION     DAZGQL010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..996
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.333"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..473)
                     /locus_tag="ACHKIR_005356"
     CDS             complement(<1..473)
                     /locus_tag="ACHKIR_005356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992928.1"
                     /translation="MVMVLTGAGMGSAATGGVGMAGASTGTAVFPGAGSRDGDWEEDN
                     ISYPGSGSGSRQGNGGFYYQGKQYDPILNEPMVVSAETHGDFWEDDMDHSETVNYEGI
                     DSKAEVQAKTDEKTCENCPPEGKVMPMIRRCSRWSMVTISYQTRICGTFYNPETQ"
     gene            complement(473..817)
                     /locus_tag="ACHKIR_005357"
     CDS             complement(473..817)
                     /locus_tag="ACHKIR_005357"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992929.1"
                     /translation="MLKLSEEQCVGMDKREDDDFVRDTALRLKKKFPGITESDDTFHV
                     RLRKALDYANTLPLKEKNVKRDFLFLEAFWPGFYLKPEVDKWLRTPNAYSTDQRVEDY
                     KHVMINRERREL"
     gene            complement(811..>996)
                     /locus_tag="ACHKIR_005358"
     CDS             complement(811..>996)
                     /locus_tag="ACHKIR_005358"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992930.1"
                     /translation="MNTELPDGRIALLRFHDPRVQKRLGEMLNDQQHRELTGLMQEWL
                     TIVDGKAWSFKQREFIC"
BASE COUNT          225 a          300 c          181 g          290 t
ORIGIN      
        1 tgttgcgtct ccggattata aaacgtgcca catattcttg tctggtagct gattgttacc
       61 atactccagc gcgagcaacg ccttatcatc ggcattacct tcccttccgg tgggcagttc
      121 tcacacgttt tctcatccgt cttcgcctgt acttcggctt tgctatcaat accttcatag
      181 tttactgttt ctgaatgatc catatcatct tcccagaaat caccatgcgt ctctgcgctg
      241 acaaccatcg gctcattcag aattggatca tactgtttcc cctgataata aaaccctccg
      301 ttaccctgac gcgaaccact gcctgagcca ggataagata tattatcttc ttcccagtct
      361 ccatcgcggc ttcctgctcc gggaaatact gctgttccgg ttgaggcacc agccatacca
      421 acacctccgg tagctgcgct ccccatacca gcccctgtca ggaccataac catcagagct
      481 ccctcctttc tctgtttatc atcacatgct tataatcttc aacccgctgg tcagttgaat
      541 aagcattcgg cgttctcaac catttatcca cttcgggttt cagataaaaa ccaggccaga
      601 aggcctccag aaacaagaag tcccgcttca cgtttttctc tttaagcgga agggtattgg
      661 cataatccag cgcttttctc aggcgaacat ggaacgtgtc atctgattca gtaattcccg
      721 gaaatttctt cttcagtctt agcgcggtgt cgcgaacaaa atcatcatct tcccttttgt
      781 ccataccaac acactgctct tcagacaact tcagcatatg aactcccttt gtttaaatga
      841 ccaggctttt ccgtccacta ttgtcagcca ctcctgcatt aaccccgtca gctcccgatg
      901 ttgttggtcg ttcagcattt ctcccaggcg cttctgaacg cgcggatcat ggaaacgcag
      961 caacgcaatc cgtccatccg gtagttctgt attcat
//
LOCUS       DAZGQL010000330          991 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.334, whole genome shotgun sequence.
ACCESSION   DAZGQL010000330 DAZGQL010000000
VERSION     DAZGQL010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..991
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.334"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>991
                     /locus_tag="ACHKIR_005359"
     CDS             <1..>991
                     /locus_tag="ACHKIR_005359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFO9992931.1"
                     /translation="AGGTGRNTTVTANGRQIVQAGGTASDTVIRDGGGQSLNGLAVNT
                     TLDNRGEQWVHGGGKAAGTIINLDGYQTIKHGGLATGTIVNTGAEGGPESENVSSGQM
                     VGGTAESTTINKNGRQVIWSSGMARDTLIYAGGDQTVHGEAHNTRLEGGNQYVHNGGT
                     ATETLINRDGWQVIKEGGTAAHTTINQKGKLQVNAGGKASDVTQNTGGALVTSTAATV
                     TGTNRLGAFSVVAGKADNVVLENGGRLDVLSGHTATNTRVDDGGTLDVRNGGAATTVS
                     MGNGGVLLADSGAAVSGTRSDGTAFRIGGGQADALMLEKGSSFTLNAGDTATDT"
BASE COUNT          249 a          246 c          327 g          169 t
ORIGIN      
        1 gcgggtggca caggcagaaa caccactgtc accgcaaatg gtcgtcagat tgtgcaggca
       61 ggaggaactg ccagtgatac ggttattcgt gatggcggag ggcagagcct taacggactg
      121 gcggtgaaca ccacgctgga taacagaggt gagcagtggg tacacggggg agggaaagct
      181 gccggtacaa ttattaacct ggatggttac cagaccataa aacatggcgg actggcaacc
      241 ggaaccatcg tcaacaccgg tgcagaaggt ggtccggagt ctgaaaatgt gtccagcggt
      301 cagatggtcg gagggacggc tgaatccacc accatcaaca aaaatggccg gcaggttatc
      361 tggtcttcgg ggatggcacg ggacaccctc atttacgccg gtggtgacca gacggtacac
      421 ggagaggcac ataacacccg gctggagggg ggtaaccagt atgtacacaa cggtggcacg
      481 gcaacagaga cgctgataaa ccgtgatggc tggcaggtga ttaaggaagg aggaactgcc
      541 gcgcatacca ccatcaacca gaaaggaaag ctgcaggtga atgccggcgg taaagcgtct
      601 gatgtcaccc agaacacggg cggtgcactg gttaccagta cagctgcaac cgtcaccggt
      661 acaaaccgcc tgggagcatt ctccgttgtg gcgggtaagg ctgataatgt cgtactggaa
      721 aatggcggac gtctggatgt gctgagcgga cacacagcca ctaatacccg tgtggatgat
      781 ggcggaacgc tggatgtccg caacggtggt gccgccacca ccgtatccat ggggaatggc
      841 ggtgtactgc tggccgattc cggtgccgct gtcagtggta cccggagcga cggaacggca
      901 ttccgtatcg gaggcggtca ggcggatgcc ctgatgctgg aaaaaggcag ttcattcacg
      961 ctgaacgccg gtgatacggc cacggatacc a
//
LOCUS       DAZGQL010000331          985 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.335, whole genome shotgun sequence.
ACCESSION   DAZGQL010000331 DAZGQL010000000
VERSION     DAZGQL010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..985
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.335"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..972
                     /locus_tag="ACHKIR_005360"
     CDS             <1..972
                     /locus_tag="ACHKIR_005360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3696 domain-containing protein"
                     /protein_id="HFO9992932.1"
                     /translation="NGDLVHVGDFDDIHNFSMREDDVLYLKYTFEDDTACWGYGIEEG
                     DAREELSRKNELPFIGCDKQLRTITNSLKDNFSFQYLEAERFGPRDNIPLSQHNYHEG
                     WLGKNGEHTIEVLESLVTRRRLNFSAQEDPRKHEGIINNNVFNHIEAWMSEISPGHRI
                     SPRIEARANVAFNVIIPSPGQLTKPINIGFGFSYALSIVTALMLASKNELVIIENPEA
                     HLHPRGQSYLGRLIALSAKAGIQVIIETHSDHLLNGIRVIARTDPTFDASLFTLYYIS
                     RENDETSVAKIKINNEGKLSEWPRGFFDQQSQDMYTIMTGSFDHPNI"
BASE COUNT          327 a          153 c          197 g          308 t
ORIGIN      
        1 aatggcgatt tagttcatgt cggagacttc gacgatattc ataatttcag tatgcgtgag
       61 gatgatgttt tatatttaaa atatacattt gaagacgata cggcttgctg gggatatggt
      121 atagaagaag gggatgctag agaagaactt tctaggaaaa atgaattacc ttttattggg
      181 tgtgataaac aattaagaac tataacaaac tcacttaaag ataacttttc attccaatat
      241 ttggaagcgg agagatttgg tccaagagat aatattccat tatcacagca caattatcat
      301 gaaggttggc ttgggaaaaa tggggaacat acaattgaag ttcttgagag tttggtcaca
      361 aggaggcgtt taaatttttc tgctcaagag gatcctagga aacatgaagg aattattaat
      421 aataatgttt tcaatcatat tgaagcatgg atgtctgaga tttcaccagg gcatcgtatc
      481 tcacctagaa ttgaggctag ggcgaatgtt gcatttaatg tcataatccc ttcacctgga
      541 caattaacga aacctattaa cataggattt ggatttagtt atgctcttag tattgtcact
      601 gctttaatgc tggcatcaaa aaatgaactc gttattattg aaaatcctga ggctcatcta
      661 catccaagag gtcaaagcta tttaggacga cttattgctt tatcagcaaa agcagggatc
      721 caggttatta ttgagactca tagtgatcat cttcttaatg gcataagagt aattgcacgc
      781 acagatccaa cttttgatgc ttctcttttt actctttact atatttcacg agaaaatgat
      841 gaaacatctg tcgccaaaat aaaaataaat aatgaaggga aactttccga atggcctaga
      901 gggttctttg atcaacaatc acaggatatg tatacgataa tgacaggctc ttttgatcat
      961 cccaatattt aatattgtag ggaca
//
LOCUS       DAZGQL010000332          974 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.336, whole genome shotgun sequence.
ACCESSION   DAZGQL010000332 DAZGQL010000000
VERSION     DAZGQL010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..974
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.336"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>974
                     /locus_tag="ACHKIR_005361"
     CDS             <1..>974
                     /locus_tag="ACHKIR_005361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFO9992933.1"
                     /translation="IVGLQVDAEQFGGQQLTVNYHIRGRIIQVPSNYDPEKRTYSGIW
                     DGSLKPAYSNNPAWCLWDMLTHPRYGMGKRLGAADVDKWALYAIGQYCDQTVPDGFGG
                     TEPRMTFNAYLSQQRKVWDVLGDFCSAMRCMPVWNGQTLTFVQDRPSDVVWPYTNSDV
                     VVDDNGVGFRYSFSALKDRHTAVEVNYTDPQNGWQTSTELVEDPDGILRYGRNLLKMD
                     AFGCTSRGQAHRAGLWVIKTELLETQTVDFTLGSQGLRHTPGDIIEICDNDYAGTLTG
                     GRILSIDAASRTLTLDREVILLEAGTSMVNLINGSGKPVRVDITAHPAP"
BASE COUNT          202 a          271 c          323 g          178 t
ORIGIN      
        1 attgtggggc tgcaggtgga tgcggagcag tttggtggcc agcagctgac ggtgaactac
       61 catatccgtg gtcgcatcat tcaggtgccg tcaaactatg acccggaaaa acgcacctac
      121 agcggtatct gggacggcag tctgaaaccg gcatacagca ataacccggc ctggtgcctg
      181 tgggacatgc tgacccaccc gcgctacggc atgggaaaac gcctgggggc cgcggatgtg
      241 gacaagtggg cactgtatgc catcgggcag tactgtgacc agacggtccc ggatggtttc
      301 gggggcacag agccgcggat gacttttaat gcgtacctgt cacagcagcg taaggtctgg
      361 gatgtcctgg gggatttctg ctcggcgatg cgctgtatgc cggtatggaa cggccagacg
      421 ctgacgttcg ttcaggaccg cccgtcggat gtggtgtggc cctacaccaa cagcgatgtg
      481 gtggtggatg ataacggcgt ggggttccgc tacagcttca gtgccctgaa ggaccggcac
      541 acggcggtgg aggtgaatta caccgacccg cagaacggct ggcagacttc cacggaactg
      601 gtggaagacc cggacggcat cctgcgctac gggcgcaacc tgctgaagat ggatgcgttc
      661 ggctgtacca gccgcggtca ggcccaccgt gccggactgt gggtgataaa gaccgaactg
      721 ctggaaacgc agacggtgga tttcacgctc gggtcacagg ggctgcgtca cacacccggt
      781 gacatcattg aaatctgtga taacgactat gccgggaccc tgaccggcgg acgcatcctg
      841 tccatcgatg ccgccagccg taccctgacg ctggaccgtg aggtgatact gctggaagca
      901 gggacatcga tggtgaacct gattaacggc agcggtaagc cggtgcgcgt ggacatcact
      961 gcacaccccg cccc
//
LOCUS       DAZGQL010000333          968 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.337, whole genome shotgun sequence.
ACCESSION   DAZGQL010000333 DAZGQL010000000
VERSION     DAZGQL010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..968
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.337"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(108..>968)
                     /gene="cynX"
                     /locus_tag="ACHKIR_005362"
     CDS             complement(108..>968)
                     /gene="cynX"
                     /locus_tag="ACHKIR_005362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanate transporter CynX"
                     /protein_id="HFO9992934.1"
                     /translation="GVGIGIIQAVMPSVIKRRFQHRTPLVMGLWSAALMGGGGLGAAI
                     TPWLVQHSETWYQTLAWWALPAVVALFAWWWQNAREVASSHKTTTTPVRVVFTPRAWT
                     LGVYFGLINGGYASLIAWLPAFYIEIGASAQYSGSLLALMTLGQAAGALLMPAMARHQ
                     DRRKLLMLALALQLVGFCGFIWLPLQLPVFWAMVCGLGLGGAFPLCLLLALDHSAQPA
                     IAGKLVAFMQGIGFIIAGLAPWFSGVLRSISGNYLMDWAFHALCVVGLMIITLRFAPA
                     RFPQLWVKEA"
BASE COUNT          273 a          301 c          242 g          152 t
ORIGIN      
        1 tttaaattat aaaaattgcc tgatgcgctc cgcttatcag gcctacaagt tcagtgatct
       61 acgtaggccg catccaacgt aaacaaagcg caggaacaag cgtcgcatca tgcctctttg
      121 acccacagct gcggaaaacg tgctggtgca aaacgcaggg ttatgatcat caacccaacg
      181 acgcacagcg catgaaacgc ccagtccatc aagtaattgc cactgatact acgcagcaca
      241 ccagaaaacc acggggcaag cccggcgatg ataaaaccga tcccctgcat aaacgccacc
      301 agtttgccag caatagcggg ttgcgcagag tgatcgagtg ccagcagcaa acagagcgga
      361 aatgcgccgc ccagacctaa accacacacc atcgcccaga agaccggcaa ttgcagcggc
      421 agccagataa agccgcagaa acccaccagt tgtaacgcca gcgccagcat caaaagtttg
      481 cgccgatcct gatggcgagc catagcaggc atcagcaatg cccccgcagc ttgcccaagc
      541 gtcatcaacg ccagcaagga accgctgtac tgcgcgctgg caccaatctc aatatagaaa
      601 gcgggtaacc aggcaatcag gctggcgtaa ccaccgttaa tcagaccgaa gtaaacaccc
      661 agcgtccacg cgcggggagt aaataccacg cgaaccggag tggttgttgt cttgtgagaa
      721 gaggcgacct cgcgggcgtt ttgccaccac caggcaaaga gcgcaacaac ggcaggcagc
      781 gcccaccagg cgagtgtttg ataccaggtt tcgctatgtt gaactaacca gggcgtaatg
      841 gcggcaccaa gtccaccgcc gcccattaga gccgcggacc acagccccat caccagtggc
      901 gtacggtgct gaaaccgccg cttaattacc gaaggcatca ctgcctgaat gatgccgatc
      961 cccactcc
//
LOCUS       DAZGQL010000334          953 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.338, whole genome shotgun sequence.
ACCESSION   DAZGQL010000334 DAZGQL010000000
VERSION     DAZGQL010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..953
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.338"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..229)
                     /locus_tag="ACHKIR_005363"
     CDS             complement(<1..229)
                     /locus_tag="ACHKIR_005363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001210963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HFO9992935.1"
                     /translation="MKEYINPGKFIISGMQTDNVVIISSSRISEIVGFLYKNNISDTN
                     TLYFSSPEALRFLWGVVDSPVYDIRHLTERCS"
     gene            complement(325..801)
                     /locus_tag="ACHKIR_005364"
     CDS             complement(325..801)
                     /locus_tag="ACHKIR_005364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001165712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HFO9992936.1"
                     /translation="MQIIIMTRDRYLEYGLMCMLNGYRLTTGRELFDAGKRRLPLPED
                     SYVILCDRNLERLTYCMFCGRRFLVIPVSSVRCLTDIRQAIRRGAWLFGHTARPLTRT
                     EMVVVFGVVFHEYGFTFLADQLGISMKTVCAHLYNAMEKNGLRGVSIKYLCSTADR"
BASE COUNT          273 a          265 c          180 g          235 t
ORIGIN      
        1 ctgaacatcg ctcagtaaga tgtctgatat cataaaccgg actgtcaacc actccccaga
       61 gaaaacggag cgcttccgga gaagaaaaat aaagggtgtt cgtatcactt atattattct
      121 tatacagaaa accgacaatt tcagatatcc gggaagaaga tattatcacc acattatctg
      181 tctgcatgcc gctgataatg aatttaccgg gattaatata ctccttcatt ccctccagaa
      241 aataactgtt atcacaaata aaatgcatac ccgttccaca tgaaatttat cacgcctggc
      301 cacataacat taattatccc ggatttaccg gtctgcggtg ctgcagagat atttaatact
      361 gacgccgcgc agaccatttt tctccatcgc attgtaaaga tgcgcacata ccgtcttcat
      421 gcttatcccc agctggtctg ccagaaaggt aaaaccgtac tcatggaaaa caactccgaa
      481 gaccaccacc atctcggtac gggtcagtgg tcttgccgta tgtccgaaca gccacgcccc
      541 acgacggatg gcctgcctga tatctgtcag gcatctcaca gaggaaacag gaatgacaag
      601 aaaacgacgc ccacagaaca tacagtatgt aagcctttcc agattacggt cacagagaat
      661 cacataactg tcttcgggaa gtggcagacg gcgctttccg gcatcaaaca gttcacggcc
      721 tgtcgtgagc ctgtatccgt tcagcataca catcaggcca tattccagat atctgtcacg
      781 ggtcataata ataatctgca tactctcaga cccttatctc ccggataccg gctccgttta
      841 ccattaccgg tataactgac tcatcattca taacggaaac tgtctgccgc atacatcagt
      901 gcccgctcct ccagctctga cagactcagc tctttcaggt ccccttctcc ttc
//
LOCUS       DAZGQL010000335          945 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.339, whole genome shotgun sequence.
ACCESSION   DAZGQL010000335 DAZGQL010000000
VERSION     DAZGQL010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..945
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.339"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            81..317
                     /gene="ycgZ"
                     /locus_tag="ACHKIR_005365"
     CDS             81..317
                     /gene="ycgZ"
                     /locus_tag="ACHKIR_005365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein YcgZ"
                     /protein_id="HFO9992937.1"
                     /translation="MHQNSVTLDSAGAITRYFAKANLPTQQETLGEIVTEILKDGRIL
                     SRKSLCAKLLCRLEQATGEEEQKHYNALIGLLFE"
     gene            360..632
                     /locus_tag="ACHKIR_005366"
     CDS             360..632
                     /locus_tag="ACHKIR_005366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YmgA"
                     /protein_id="HFO9992938.1"
                     /translation="MKTSDNESTKYAITGQAVLQILRMKINFSLQTLIKQLLVMKSVE
                     ENAFRRDLIDSIIRDFSNSDTGGPNRRTAITDNKSIFNGKKINRIH"
     gene            661..927
                     /gene="ariR"
                     /locus_tag="ACHKIR_005367"
                     /gene_synonym="ymgB"
     CDS             661..927
                     /gene="ariR"
                     /locus_tag="ACHKIR_005367"
                     /gene_synonym="ymgB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm/acid-resistance regulator AriR"
                     /protein_id="HFO9992939.1"
                     /translation="MLEDTTIHNAIADKALSNYFRSSGNLLEEESAVLGQAVTNLMLS
                     GDNVNNKNIILSLIHSLETTSDILKADVIRKTLEIVLRYTADDM"
BASE COUNT          323 a          152 c          192 g          278 t
ORIGIN      
        1 cattctgtac acatatttcg tacaagtttg ctattgttac ctcacttaac attgattaac
       61 atttttaaca gaggcgtagc atgcatcaaa attcagtgac tttagattct gcgggagcaa
      121 tcactcgtta ctttgcaaaa gctaacttgc ctactcagca ggaaactctc ggggaaattg
      181 tgactgagat tttgaaagat ggacgtattc tgagtcgaaa gtcgctttgt gccaaacttc
      241 tatgccgact ggaacaagcg acaggggaag aggaacagaa acattataat gcactaattg
      301 ggttgctttt tgaataatgc aacagcctga ttatttaaca ggttagctat gaagtcgtta
      361 tgaagacatc tgataatgaa agtacaaaat atgcaattac tggccaggcg gtgctccaga
      421 tactgcgcat gaagataaat ttttcgttgc agactcttat taagcaatta ctcgtaatga
      481 aatctgttga ggaaaatgct ttccgacgag atttaattga cagcataatt cgtgatttta
      541 gcaacagtga tacaggaggg ccaaacagaa gaacagcgat aactgacaat aaaagtattt
      601 tcaatggtaa gaaaataaac agaatacatt aaaatttcat aagtaagatg agaggttact
      661 atgcttgaag atactactat tcataatgca atagctgata aagcgttatc aaattacttt
      721 cgcagttcgg gtaatttgtt agaagaagag tcagctgtgt tagggcaggc tgtcaccaat
      781 ttaatgcttt caggtgataa tgttaataat aaaaatatta tcctaagtct gatacactcc
      841 ctggaaacaa caagtgatat tctcaaagct gatgtgatta gaaaaacact ggaaatcgtg
      901 ttgcgataca cagcggatga tatgtgactt ttaattatta agtat
//
LOCUS       DAZGQL010000336          926 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.340, whole genome shotgun sequence.
ACCESSION   DAZGQL010000336 DAZGQL010000000
VERSION     DAZGQL010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..926
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.340"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..263
                     /locus_tag="ACHKIR_005368"
     CDS             <1..263
                     /locus_tag="ACHKIR_005368"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992940.1"
                     /translation="KRRTGMEEMQDKMAWMQNLITEKLGPSLWHMHPIAFPGALIKKN
                     TGWAHSKFANLLGSVESENDYSAYNVTKTGKSVPHYNINLTK"
     gene            434..>926
                     /locus_tag="ACHKIR_005369"
     CDS             434..>926
                     /locus_tag="ACHKIR_005369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992941.1"
                     /translation="MGCNKKSNKFLLFVSCMLLSFVTYSQDSSNPQFSDYLVPVSNGP
                     FEKNIHFNKEQENYSQHWKNAVQEELKKPVNFAGHFRIYTAFGGHGKECLRDNWVCGW
                     VIDKLSGEVVATLPSDNNGSYNYADVSDNGTPVGLPFEIDTYKNSSMIAITGQSISVS
                     KSDN"
BASE COUNT          307 a          153 c          206 g          260 t
ORIGIN      
        1 aaaaaagacg taccggaatg gaagaaatgc aggataagat ggcctggatg cagaatctga
       61 tcacggagaa actgggccct tcgttgtggc atatgcatcc gattgccttt cctggagcat
      121 taataaagaa aaatactggg tgggcgcata gcaagtttgc aaaccttttg ggaagtgtcg
      181 agtcagaaaa tgattattcg gcatataatg ttactaagac aggtaaatct gtcccgcact
      241 ataatattaa tctcacaaaa tgacattatc tgaggtaatt gatgccccaa gagaacgaaa
      301 aatggtaaaa agaaatacag gttagtacct agggtggcag aagttgaggg ggagtcgtac
      361 tattctggtg atggattgaa taaagctcac ctattaccag atacgatgat taaagctctt
      421 gaggattcta aaaatgggat gcaataaaaa atcaaacaaa tttttgcttt ttgtgtcatg
      481 catgctcttg tcgtttgtaa cgtactcaca agacagttct aatcctcagt tttctgacta
      541 cctggttcct gtaagcaacg gaccatttga aaagaatatt cactttaata aagagcagga
      601 aaattactcg caacactgga agaatgcagt ccaagaggaa ctaaaaaagc ctgttaattt
      661 tgcagggcac tttagaatct atacagcctt tggcggtcat ggtaaagaat gccttcgtga
      721 taattgggta tgtggttggg ttattgataa actatcaggg gaagttgtag caacacttcc
      781 atcagataat aatggaagtt ataactatgc tgatgtgtct gataatggta cccctgttgg
      841 tcttccattt gagattgata cttataaaaa tagttcaatg attgcaataa ccggtcaatc
      901 tatctcagta agcaagagtg ataatc
//
LOCUS       DAZGQL010000337          924 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.341, whole genome shotgun sequence.
ACCESSION   DAZGQL010000337 DAZGQL010000000
VERSION     DAZGQL010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..924
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.341"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            1..402
                     /locus_tag="ACHKIR_005370"
                     /pseudo
     CDS             1..402
                     /locus_tag="ACHKIR_005370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308632.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            394..729
                     /locus_tag="ACHKIR_005371"
     CDS             394..729
                     /locus_tag="ACHKIR_005371"
                     /inference="COORDINATES: protein motif:HMM:NF026979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="papain fold toxin domain-containing protein"
                     /protein_id="HFO9992942.1"
                     /translation="MAKTRDLATTISDKFKQLFQCDKFAAALKSKMQQAGVNGEHLIL
                     ETKNKQGAFGNIWSDRIGKNIATNGHHEAIKVGDTVFDNMNPSGIKYEDWLNDIYSPG
                     EYTITPSRF"
     gene            732..>924
                     /locus_tag="ACHKIR_005372"
     CDS             732..>924
                     /locus_tag="ACHKIR_005372"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992943.1"
                     /translation="MNDIVKLIDMIEKRPALYLSRHTISALKAFLDGWQMREPKEIVN
                     IEILIRLQSYIEDYYGVTGH"
BASE COUNT          290 a          178 c          230 g          226 t
ORIGIN      
        1 ccgtcggtgg ccccgcagtg ggtgacgcgg acgaatcatg cggtgagcga cctgacgggc
       61 cgtccgctga tgctctttaa cagtgaaggt aaaaccgtgt ggcgaccgtg acaaaccagc
      121 ctgtgggggc tggcactcag tctgcccgca gacaccggct acccggaccc gcgcggggag
      181 ctggacccgg aagccgaccc cggcctgctg tatgcaggac agtggcagga tacggaatcc
      241 gggctgtgct ataaccggtt ccggtattac gagccggaaa cagggatgta cctggtgagt
      301 gacccgatag ggctgagagg cgggataaac aattatgcat atgttcataa tccccttggt
      361 tatattgatc cattaggatt aaccatttgc gatatcgcaa aaacaaggga tcttgcaaca
      421 acaataagtg ataagtttaa acagcttttt caatgcgata aatttgccgc agcgttaaaa
      481 agtaaaatgc aacaagctgg ggtaaatggt gagcatctca tactcgaaac taaaaataaa
      541 caaggtgcct ttggcaatat atggagtgat agaataggta aaaatatagc aactaatggt
      601 catcatgaag caataaaagt aggtgataca gtttttgata atatgaatcc ttctggtata
      661 aagtatgaag attggctgaa tgatatatac tctcctggcg aatacaccat tactccatca
      721 aggttttaat tatgaatgat attgttaaac tgattgatat gatagaaaaa agaccagctt
      781 tatatttaag caggcatacc atatcggcgt taaaagcttt cctggatgga tggcaaatga
      841 gagagccaaa agaaatcgtc aatattgaga tattaataag gcttcaatct tatattgaag
      901 actattatgg agtaacagga catt
//
LOCUS       DAZGQL010000338          908 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.342, whole genome shotgun sequence.
ACCESSION   DAZGQL010000338 DAZGQL010000000
VERSION     DAZGQL010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..908
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.342"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            68..592
                     /gene="tssJ"
                     /locus_tag="ACHKIR_005373"
     CDS             68..592
                     /gene="tssJ"
                     /locus_tag="ACHKIR_005373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFO9992944.1"
                     /translation="MVTVIRWWVPMFIACGLLAGCGLTQTVTDGTISITKSIFYKKIK
                     TLHLDFTPRTAINTDGAQTPLATMVRVYQLRDRKAVDVAGYQMLLRNADTVLKDDVLA
                     SKELLVMPGGSVTLNMPMDEDAQFVAVVGLFNRPDQKDNRWRLVLTRDDLNPDKPRTI
                     ELGDGWLSLVPVKE"
     gene            597..>908
                     /locus_tag="ACHKIR_005374"
     CDS             597..>908
                     /locus_tag="ACHKIR_005374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contractile injection system protein, VgrG/Pvc8
                     family"
                     /protein_id="HFO9992945.1"
                     /translation="MDNRSAFFDGQTRYFLEINGSAVKPDVLRFRGREALSEPFRWDI
                     GFTSRNANIPPEEVLMKYASLRMRNGKNVHGIVTRLEWLSTSKDQSHYRLTLSSRLAL
                     LE"
BASE COUNT          208 a          233 c          259 g          208 t
ORIGIN      
        1 ctgctgaaga cggacattat cgcttcgaat aatcacctgt tctctctctc aataaaggag
       61 cgcctgaatg gtaacagtaa tccgctggtg ggtgccgatg tttattgcct gtggcctgct
      121 cgcaggctgc ggtctgacgc agaccgtcac ggacggcacg atcagcatca cgaaatccat
      181 cttctacaaa aaaatcaaaa cgctgcatct ggattttacc ccacgcacgg ccattaacac
      241 tgacggggca cagacgccac tggcaaccat ggtgcgggtt taccagctca gggatcgtaa
      301 agccgttgat gtcgccggtt accagatgct gctgcgtaat gccgatacgg tacttaagga
      361 cgacgtgctg gcctcaaaag agctgctggt gatgcccggc ggcagcgtga cgctcaatat
      421 gcccatggat gaagatgcgc agtttgtggc tgtggtggga ttgtttaacc ggccggatca
      481 gaaggacaat cgctggcgcc tggtactgac ccgtgatgac ctcaatccgg ataagccccg
      541 caccattgaa ctgggtgatg gctggctcag ccttgtgccg gtaaaggagt gacgtgatgg
      601 ataaccggtc agcgtttttt gacgggcaga cccgttattt tctggaaatt aatggcagtg
      661 cagtaaagcc tgatgtttta cgttttcgtg gcagggaagc actgagtgag ccgttccgat
      721 gggacatcgg gttcacctcc cggaatgcga acattcctcc ggaagaggtg ctgatgaaat
      781 atgcgtcgtt gcggatgcgt aacggcaaaa atgtccatgg cattgtgacc cgtctggaat
      841 ggctgtccac atcgaaagac cagtcccact accggcttac actcagctcc cgtctggcac
      901 tgctggag
//
LOCUS       DAZGQL010000339          906 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.343, whole genome shotgun sequence.
ACCESSION   DAZGQL010000339 DAZGQL010000000
VERSION     DAZGQL010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..906
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.343"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            66..590
                     /gene="tssJ"
                     /locus_tag="ACHKIR_005375"
     CDS             66..590
                     /gene="tssJ"
                     /locus_tag="ACHKIR_005375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFO9992946.1"
                     /translation="MVTVIRWWAPMFIACGLLAGCGLTQTVTDGTISITKSIFYKKIK
                     TLHLDFTPRTAINTDGAQTPLATMVRVYQLRDRKAVDAADYQMLLRRADTVLKDDVLA
                     SKELLVMPDGSVTLNMPMDEDAQFVAVVGLFNRPDQKDNRWRLILTRDDLNPDKPRTI
                     ELGDGWLSLVPVKE"
     gene            595..>906
                     /locus_tag="ACHKIR_005376"
     CDS             595..>906
                     /locus_tag="ACHKIR_005376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contractile injection system protein, VgrG/Pvc8
                     family"
                     /protein_id="HFO9992947.1"
                     /translation="MDNRSAFFDGQPRYFLEINGSAVKPDVLRFRGREALSEPFRWDI
                     GFTSRNANIPPEEVLMKYASLRMRNGKNVHGIVTRLEWLSTSKDQSHYRLTLSSRLAL
                     LE"
BASE COUNT          209 a          232 c          258 g          207 t
ORIGIN      
        1 ctgctgaaga cgggcattat cgcttcgaat aatcacctgt tctctctcaa taaaggaacg
       61 cctgaatggt aacagtaatc cgctggtggg cgccgatgtt tattgcctgt ggcctgctcg
      121 caggctgcgg tctgacgcag accgtcacgg acggtacgat cagcatcacg aaatccatct
      181 tctacaaaaa aatcaaaacg ctgcatctgg attttacccc gcgtacggcc attaacactg
      241 atggggcaca gacgccactg gcaaccatgg tgcgggttta tcagctcagg gatcgtaaag
      301 cggttgatgc cgccgattac cagatgctgc tgcgccgggc cgatacggta cttaaggacg
      361 acgtgctggc ctcaaaagaa ctgctggtga tgcccgacgg cagcgtgacg ctcaatatgc
      421 ccatggatga agatgcgcag tttgtggctg tggtgggact gtttaaccgg ccggatcaga
      481 aggacaatcg ctggcgcctg atactgaccc gtgatgacct caatccggat aagccccgca
      541 ccattgaact gggtgatggc tggctcagtc ttgtgccggt aaaggagtga cgtgatggat
      601 aaccggtcag cgttttttga cggacagccc cgttactttc tggaaattaa tggcagtgca
      661 gtaaagcctg atgttttacg ttttcgtggc agggaagcac tgagtgagcc gttccgatgg
      721 gatatcgggt tcacctcccg gaatgcgaac attcctccgg aagaggtgct gatgaaatat
      781 gcgtcgttgc ggatgcgtaa cggcaaaaat gtccatggca ttgtgacccg tctggaatgg
      841 ctgtccacat cgaaagacca gtcccactac cggcttacac tcagctcccg tctggcactg
      901 ctggag
//
LOCUS       DAZGQL010000340          901 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.344, whole genome shotgun sequence.
ACCESSION   DAZGQL010000340 DAZGQL010000000
VERSION     DAZGQL010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..901
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.344"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..165)
                     /locus_tag="ACHKIR_005377"
     CDS             complement(<1..165)
                     /locus_tag="ACHKIR_005377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFO9992948.1"
                     /translation="MNSTTAAIMNYDPNMTLCGRMARQTVRLTLGQWEYRETFEVDVV
                     GNLTGLDVITC"
     gene            complement(158..>901)
                     /locus_tag="ACHKIR_005378"
     CDS             complement(158..>901)
                     /locus_tag="ACHKIR_005378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HFO9992949.1"
                     /translation="IRQNHSRNEERFVVGKHYFLLEGDELRELKHRVSQSYSVKIASN
                     VRSLILWTERGAANHAKLLETDQAWNYHEDLVEFYFTQRENIDVQRVLTRKELALMVI
                     EAEERAEAMALENKTLSATVDSLEKHFSKGITVPQFCRALNGVNTSQIMWWLSGRKWV
                     FNEQRDPEKTPRWRTASYARDRYLTEEAVQITPHGKATFTKFTPVLLEKGCHRLYQFY
                     MNGELPMKKNWNGEFKHDKAIYTPEAEHE"
BASE COUNT          187 a          220 c          223 g          271 t
ORIGIN      
        1 gcaggttatt acatccagtc ccgtcagatt gccgacaacg tccacttcaa atgtttcgcg
       61 gtactcccat tgcccgagtg tcagacgaac cgtctgtctt gccatgcgcc cgcataatgt
      121 catgttcggg tcgtagttca tgattgccgc agtagtgcta ttcatgttct gcctccggtg
      181 tgtaaatagc tttgtcgtgt ttaaattcac cgttccagtt tttcttcata gggagttcac
      241 cgttcatgta aaactggtat aaacggtggc atcctttttc cagcaatacg ggggtgaatt
      301 tcgtgaatgt tgctttcccg tgtggagtga tctgtactgc ttcttcggtc aggtatctgt
      361 cacgggcata agaggccgtg cgccagcggg gtgttttctc cggatcgcgt tgttcgttga
      421 atacccattt tcggccagac agccaccaca ttatctggct ggtatttacg ccgttcagcg
      481 cacggcagaa ctggggaaca gttatgcctt tagagaaatg tttttccaga ctgtctacgg
      541 ttgcagagag cgttttattt tccagggcca tagcttcagc ccgttcttcg gcctcaataa
      601 ccatcaacgc gagttccttg cgagtgagta cgcgctgaac atcgatgttt tcccgctgag
      661 tgaagtagaa ctccacaagg tcttcgtgat agttccacgc ctgatccgtt tccagcaatt
      721 ttgcgtggtt ggctgcgccg cgttcggtcc agaggatgag ggatcgaaca ttgctggcaa
      781 ttttcacaga gtagctttga gatactctgt gcttaagttc acgtagttca tcgccttcca
      841 gcaaaaaata atgctttcca acaacgaatc gttcttcgtt tcgggaatga ttctggcgaa
      901 t
//
LOCUS       DAZGQL010000341          897 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.345, whole genome shotgun sequence.
ACCESSION   DAZGQL010000341 DAZGQL010000000
VERSION     DAZGQL010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..897
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.345"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..602
                     /locus_tag="ACHKIR_005379"
     CDS             <1..602
                     /locus_tag="ACHKIR_005379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion IcmF C-terminal
                     domain-containing protein"
                     /protein_id="HFO9992950.1"
                     /translation="DSGRITTFLKTHLGGLVHQEGNHWVAEPSASQGMVVSPDFLRAI
                     NQLAELSDIVFSQGDAGVHFELMARPSRDVARVQLTLDEQNLDYFNQMESWQSFTWPG
                     NTYYPGVSLSWRSVSSGMQLYASHQGNWGFIRLLDEALITPLDASRTQLVWITADGNP
                     LKFVMRSELGDGPLALLKLQGFTLPTAIFNVAAGTEGVE"
     gene            605..886
                     /locus_tag="ACHKIR_005380"
     CDS             605..886
                     /locus_tag="ACHKIR_005380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992951.1"
                     /translation="MNRGIYPTPAFTEEPGADGHIFPLSENGSFTPVGVFAPMSEKTS
                     GKRPEGQSHSAGESMLLKWTPPQIVNSKDDASPEAVVRFMKETDKQEKE"
BASE COUNT          209 a          234 c          261 g          193 t
ORIGIN      
        1 gcgactcggg tcggatcacg acgttcctga aaactcatct tggcgggctc gtccaccagg
       61 aggggaatca ctgggtcgct gagccttccg caagccaggg gatggttgtc agtccggatt
      121 tcctccgggc gattaatcag ctggcagagc tgtcagatat cgtgttctct cagggcgatg
      181 cgggggttca cttcgaactg atggcgcgtc cttcacggga tgttgccaga gtgcagctta
      241 cccttgatga acagaatctg gattacttca atcagatgga aagctggcag agcttcacct
      301 ggccggggaa tacttactat ccaggtgtgt cgctcagctg gcggagtgta agcagtggta
      361 tgcaactcta cgccagtcac caggggaact gggggtttat tcgcctgctg gacgaagccc
      421 tgatcacgcc acttgatgcc agccgcacac aactggtgtg gatcactgcc gatggtaatc
      481 ctctgaaatt cgtcatgcgc agcgagctgg gcgacggtcc gcttgcctta ctgaagctac
      541 aggggtttac gctgcccacc gccattttta acgttgccgc cggcacggaa ggtgttgagt
      601 aaaaatgaac aggggcattt atccgacgcc ggcctttact gaagaaccgg gggctgatgg
      661 gcacattttc cccctttcag agaacggttc cttcacgccc gtcggcgttt tcgcccccat
      721 gtcagaaaaa acaagcggta agcgaccgga agggcaatcc cactcagcag gggaaagtat
      781 gctgctgaaa tggacgccac cacaaatcgt caacagtaag gacgacgctt ctccggaagc
      841 ggtcgtgagg tttatgaaag agactgacaa acaggaaaaa gaatgagttc acctgaa
//
LOCUS       DAZGQL010000342          892 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.346, whole genome shotgun sequence.
ACCESSION   DAZGQL010000342 DAZGQL010000000
VERSION     DAZGQL010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..892
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.346"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..438
                     /locus_tag="ACHKIR_005381"
     CDS             <1..438
                     /locus_tag="ACHKIR_005381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFO9992952.1"
                     /translation="IEDKINDSNMAIINLNIERNNITNILKLAKPLVSSPFIIRNIAV
                     SNGQYVNSGDDIVDIELLDKFFIDVKIDPVSEQGNIKEKKIRYHSLVNGLSGLASVVR
                     ITRTFENGQDDKSSGLRVVTLAIDGDHKKLAELLDTAFEIEIK"
     gene            435..>892
                     /locus_tag="ACHKIR_005382"
     CDS             435..>892
                     /locus_tag="ACHKIR_005382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFO9992953.1"
                     /translation="MIKLDIYEKQFKDKKILSSISVNVEKGSFHVITGPSGVGKSSLL
                     NILGLLDSEFDGNYEFCGKNINPKDYNKTSRIRKEYFGFIFQDSLINEKQSVLRNILC
                     SVDYPYLKFAEKNAHTMLEIVGLPMPDMKVMNLSGGEKQRLALARALIKKP"
BASE COUNT          311 a          125 c          172 g          284 t
ORIGIN      
        1 atagaagata aaataaatga ttcgaacatg gctattataa atctgaatat agagagaaat
       61 aatattacta atatattaaa acttgcaaaa ccgttggtat catcgccttt tatcataagg
      121 aatattgctg taagcaatgg gcaatatgtc aattcaggag acgatatcgt agatattgaa
      181 cttctggata agtttttcat tgatgttaaa attgatcctg tgtctgaaca aggaaatatc
      241 aaagaaaaaa agataagata tcactctttg gtcaatggat tatccggttt agcatctgta
      301 gtccgcatca cccgtacctt cgaaaatggc caggatgata aaagttctgg tttgagggtt
      361 gtaacattgg ccattgatgg tgatcataaa aaactcgctg aattactcga tactgctttt
      421 gagattgaaa taaagtgatt aaattagata tttatgaaaa acagttcaag gataaaaaaa
      481 tccttagttc tatatctgtt aatgttgaaa aaggttcatt tcatgttatt acagggcctt
      541 ccggggtcgg aaagagttca ctgttaaaca tacttggttt gttggatagt gaatttgatg
      601 ggaattatga gttttgtggt aaaaatatta atccgaagga ttacaataaa acttctcgta
      661 taaggaagga gtattttggt tttatatttc aagattctct cattaatgaa aaacaaagtg
      721 tactacgtaa cattctatgc tccgtagatt atccttattt aaaattcgca gagaaaaatg
      781 cgcataccat gcttgaaatc gttggactcc ctatgcctga tatgaaggtt atgaaccttt
      841 cagggggaga aaaacagcgt cttgctcttg caagagcttt aataaaaaaa cc
//
LOCUS       DAZGQL010000343          885 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.347, whole genome shotgun sequence.
ACCESSION   DAZGQL010000343 DAZGQL010000000
VERSION     DAZGQL010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..885
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.347"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>885
                     /locus_tag="ACHKIR_005383"
     CDS             <1..>885
                     /locus_tag="ACHKIR_005383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pertactin-like passenger domain-containing
                     protein"
                     /protein_id="HFO9992954.1"
                     /translation="GTTTLNNGATLTLSGKTVNNDTLTIHEGDALLQGGALTGNGRVE
                     KSGSGTLTVSNTTLIQKTVNLNEGTLTLNDSTVTTDVIAQRGTALKLTGSTVLNGAID
                     PTNVTLTSGATWNIPDNATVQSVVDDLSHAGQIHFTSARTGKFVPTTLQVKNLNGQNG
                     TISLRVRPDMAQNNADRLVIDGGRATGKTILNLVNAGNSGTGLATTGKGIQVIEAING
                     ATTEEGAFVQGNMLQAGAFNYTLNRDSDESWYLRSEERYRAEVPLYASMLTQAMDYDR
                     ILAGSRSHQTGVNGENNSV"
BASE COUNT          226 a          256 c          249 g          154 t
ORIGIN      
        1 gggcaccacc acgctgaata acggtgccac gcttaccctt tccgggaaaa cggtgaataa
       61 cgataccctg accatccatg aaggtgatgc actcctgcag ggaggcgctc ttaccggtaa
      121 cggcagggtg gaaaaatcag gaagtggcac actcactgtc agcaacacca cactcatcca
      181 gaaaaccgtc aacctgaatg aaggcacgct gacgctgaac gacagtaccg tcaccacgga
      241 tgtcatcgct cagcgcggca cggccctgaa gctgaccggc agcacagtgc tgaacggtgc
      301 cattgacccc acgaatgtca ccctgacatc cggtgccacc tggaatatcc ccgataacgc
      361 cacggttcag tcggtggtgg atgacctcag ccatgccgga cagattcatt tcacctccgc
      421 ccgcacaggg aagttcgttc cgacaaccct gcaggtgaaa aacctgaacg ggcagaatgg
      481 caccatcagc ctgcgtgtac gcccggatat ggcgcagaac aatgctgaca gactggtcat
      541 tgacggtggc agggcaaccg gaaaaaccat cctgaatctg gtgaacgccg gcaacagtgg
      601 tacggggctg gcgaccaccg gtaagggtat tcaggtgatt gaggccatta acggtgccac
      661 cacggaggaa ggggcttttg tccaggggaa tatgctgcag gccggggcct ttaactacac
      721 cctcaaccgg gacagtgatg agagctggta tctgcgcagt gaagaacgtt atcgtgctga
      781 agtccccctg tacgcctcca tgctgacaca ggcaatggac tatgaccgga ttctggcagg
      841 ctcccgcagc catcagaccg gtgtaaacgg tgaaaataac agcgt
//
LOCUS       DAZGQL010000344          884 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.348, whole genome shotgun sequence.
ACCESSION   DAZGQL010000344 DAZGQL010000000
VERSION     DAZGQL010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..884
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.348"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..416)
                     /locus_tag="ACHKIR_005384"
     CDS             complement(<1..416)
                     /locus_tag="ACHKIR_005384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFO9992955.1"
                     /translation="MYTESNVKLSCNALWQKCVLATLYRAAATHPEIKLLQFKLIPGQ
                     GINKAIPSLMADFTGELHRLISEYLQCKRRKDKSVEPTPLYYLWSREAVANDTTPYLM
                     VLLLNNDVWCKSREVVVLRNLIEQAWGKVLGEDNSIT"
     gene            complement(444..641)
                     /locus_tag="ACHKIR_005385"
     CDS             complement(444..641)
                     /locus_tag="ACHKIR_005385"
                     /inference="COORDINATES: protein motif:HMM:NF023154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFO9992956.1"
                     /translation="MPQDDFCIVNPGGCIIHFPANGRYILDIHDPDFPEQYQRLLERL
                     DYLTKPGTKASGQRNSGYSGF"
BASE COUNT          258 a          210 c          197 g          219 t
ORIGIN      
        1 gtaatggagt tatcttcacc caatactttt ccccacgcct gctcaatcag atttctgaga
       61 accaccacct cacgactctt acaccagaca tcgttattaa gtagcagcac cataagataa
      121 ggagtggtat cgttagccac agcctcccta ctccagagat aatataaagg ggtgggctca
      181 acagatttat ctttacgtcg cttacactgc aaatattcag aaatgagtct atgcagttca
      241 ccagtaaaat cagccatcaa agagggaatg gccttattaa taccctgccc tggtattaat
      301 ttaaattgta ataatttaat ttcaggatgt gtggctgcag cccgatacag agttgcaagg
      361 acacactttt gccagagggc gttacaggaa agcttaacgt ttgattctgt atacataata
      421 aatcacctta cagttacaac aggtcaaaaa ccgctgtagc cagagttacg ctggcctgat
      481 gctttagtac cgggcttcgt cagataatcc agacgctcca ataagcgctg atactgctca
      541 gggaaatcag gatcatgaat atccaggatg taacgtccat tagcagggaa atgaataatg
      601 cagccccctg gattaacaat gcagaaatcg tcctgaggca ctgatcaata cggagaggac
      661 tctcgcgtgt ggtttattga caccacagtg cagattcggc gaatccgcga tcacggtgca
      721 atttcgttcc acagcacaca atcataaccc cgggttttat tcaggtaagc aggactgcgg
      781 atatccggtg tcgcgccttt gtgtcacgaa cggggtaggt gcgaaacacc ggataaaatg
      841 caggctggca atacctctga acgccctgcg cagagcggat attt
//
LOCUS       DAZGQL010000345          838 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.349, whole genome shotgun sequence.
ACCESSION   DAZGQL010000345 DAZGQL010000000
VERSION     DAZGQL010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..838
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.349"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(200..763)
                     /gene="ipbA"
                     /locus_tag="ACHKIR_005386"
                     /gene_synonym="fimX"
     CDS             complement(200..763)
                     /gene="ipbA"
                     /locus_tag="ACHKIR_005386"
                     /gene_synonym="fimX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_060710320.1"
                     /note="IpbA is also known as HbiF and FimX; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type DNA invertase IpbA"
                     /protein_id="HFO9992957.1"
                     /translation="MTRKYLTQDEVYRLMDAAQSMSFPERNRCLIMMAFIHGFRASEL
                     LDLRLSDIDASGKQLNIRRIKNGFSTTHPLLPDEYNLIKLWLKQRKLIENGVEGDWLF
                     LSRKCRPISRQHFFSIIREAGRRAGLAVKAHPHMLRHACGFALADNGVDTRLLQDYLG
                     HRNIQHTVRYTASNAARFKGVWKKKPR"
BASE COUNT          248 a          164 c          172 g          254 t
ORIGIN      
        1 tttgtgttat ttaacaacgc gagtgaaaac tgatcactgg tatgaccttt gtggttaatc
       61 atttcaaatc atggggttat tttactttag cgagaaatat caacatgtta tgtgttttat
      121 ttaattgaat gagctgtttt tttatggaag aattgaggca aacgtaggaa tctgacattt
      181 tgaatcagaa ggtactgttt taacgaggct tttttttcca caccccttta aatcgagcag
      241 cattactggc tgtgtatctg acagtatgct gaatatttcg atgacccaga taatcctgaa
      301 gtagtcgggt atcgacacca ttgtcagcca gagcaaatcc acaggcatga cgtaacatat
      361 gaggatgtgc ttttacagct aatcctgcac gtcttcctgc ctcacgaatg atcgaaaaaa
      421 aatgttgtct gctgataggg cggcatttcc gggagagaaa aagccagtct ccttcaactc
      481 cattttcaat taacttcctc tgcttaagcc acaacttgat caaattatat tcatcaggaa
      541 gaagaggatg tgttgtcgag aagccatttt taatacgccg gatattaagt tgttttcctg
      601 atgcatcaat atcggataaa cgtaaatcaa gaagttcact ggccctaaaa ccgtgaatga
      661 aagccatcat aattagacag cgatttcttt caggaaagga catgctctgg gctgcatcca
      721 tcagcctgta gacttcatcc tgtgtgagat attttctcgt catacgccac cgtgtacagt
      781 tgtaaaaaaa agaggcacac ccagataagt gtgcctgcaa tcagaacgtt atcgatgt
//
LOCUS       DAZGQL010000346          833 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.350, whole genome shotgun sequence.
ACCESSION   DAZGQL010000346 DAZGQL010000000
VERSION     DAZGQL010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..833
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.350"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..663
                     /locus_tag="ACHKIR_005387"
     CDS             <1..663
                     /locus_tag="ACHKIR_005387"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFO9992958.1"
                     /translation="VIVISEALKNKIIKYITGNNLNLIYDDVHIQNKPMLNYANSARL
                     RKFIIIGTLCEGKGQKIAIEAMHNLIREGYLCHLKIIGNNRVPYASYLNKIVADYNLS
                     DYVEFMGFRHDLDQIRLDNDVCLIPSLSEAFGRVTIESMAAGMIVVASDSGASKEIIN
                     DGINGFLFSSGSVSDLTSVLKKILDVESNNLECIRKRALVDSQKYTSGHAASSIYNLI
                     IN"
BASE COUNT          285 a          101 c          149 g          298 t
ORIGIN      
        1 gttatagtta tatcagaagc gttaaaaaat aaaataataa aatacattac aggaaataat
       61 cttaatctaa tatatgatga tgtccatatc caaaacaaac caatgctaaa ttatgctaat
      121 tcggctaggc tgcggaagtt cattattatt ggcacattgt gtgagggcaa aggtcaaaaa
      181 atagccatag aggctatgca taatctgatt cgtgaaggat atttatgtca cttaaaaata
      241 attggaaata atagagttcc atatgcaagt tatcttaata aaattgttgc tgattataat
      301 ttgagtgatt atgtagagtt tatgggattt aggcatgatc ttgatcaaat aaggcttgac
      361 aacgatgttt gcttaattcc ctctctttct gaagcttttg gtagagttac cattgaatca
      421 atggctgcag gtatgatagt tgttgctagt gattctgggg ctagtaaaga gatcatcaat
      481 gatggtataa atggtttttt gttttcttca ggttcggtaa gtgatcttac tagcgtactt
      541 aaaaaaatcc ttgatgtaga gtctaataat ttagaatgta taaggaaaag ggctttggtt
      601 gactcgcaaa aatatacatc aggacatgca gcttcatcaa tttataattt gatcattaac
      661 tgatttttta taaactttga ttatcatttt aaatgttaat taaaaaacaa taagggctaa
      721 ttagtgcaaa gtaatgttaa agggttgagc tattattggt cttttctatg ttttacaata
      781 atgatatttt tcctttttag acaataccca ttattaaagt attcatcaat tat
//
LOCUS       DAZGQL010000347          833 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.351, whole genome shotgun sequence.
ACCESSION   DAZGQL010000347 DAZGQL010000000
VERSION     DAZGQL010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..833
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.351"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            224..299
                     /locus_tag="ACHKIR_005388"
     tRNA            224..299
                     /locus_tag="ACHKIR_005388"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:257..259,aa:Ile,seq:cat)
BASE COUNT          243 a          159 c          183 g          248 t
ORIGIN      
        1 tcattacaaa ctatcattga atcgatgata actatagata gtgcttatta ttaacatgct
       61 tgttgactgc gagtatcgac tttgcgaatg gaagcggtgt cgggatttat gtcgcatgta
      121 ttgacgaccc aggatggatg agaacgcata aaaatctgtt atttacatca taagatcatc
      181 atgtttattg cgtggtttat aagtattgtt attgcatact taaggccctt tagttcagtg
      241 gttagagcag gcgactcata atcgcccggt cgctggttca agtccagcag gggccaccaa
      301 atcatcattc atgtatcaag aagagttaag ggtacaaggg gtactcgatg gcggtctata
      361 tttcatgact aacgccattt aaaggaagaa gtgcggtggg tggtttcgaa tgagggctgg
      421 tgttttggta cgtggcacgc cactgcaatg tatttatcca tcagtactca ttcaaaagta
      481 cctggaaaaa atgcccgcta tgccaagcgg gcaaattgct tacatgtgga attaagtaat
      541 taacgataaa gccagagtat tatcatttat catgcctctt catttaatca ggttaattac
      601 tcaaatcact gaactattct ctgttcgctc actgatgata agtgaagtgt cacaaccacg
      661 atttgtatgt ggaccaatgc cgtagagtaa ggatgaaatg tctctgccac gccttaaata
      721 tctactactg accagaacac ccgccgcagg cagatgagtc ttatgagcgt tacaggagtc
      781 tggctttgct cattaattaa cgctatttag atataaattt ctaacactcg aaa
//
LOCUS       DAZGQL010000348          823 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.352, whole genome shotgun sequence.
ACCESSION   DAZGQL010000348 DAZGQL010000000
VERSION     DAZGQL010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..823
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.352"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            131..>823
                     /gene="reiD"
                     /locus_tag="ACHKIR_005389"
     CDS             131..>823
                     /gene="reiD"
                     /locus_tag="ACHKIR_005389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inositol utilization transcriptional
                     regulator ReiD"
                     /protein_id="HFO9992959.1"
                     /translation="MAYVSQVSESNGPFVRFMHMHPRTVELILITEGKGEYFIGDRIY
                     PVRKGDLVIYNSEVVHDEYLESGRPVGTICCGINNIVCPGLRENALIPDDTVPVIPLY
                     HHYATIEKLMSSIFNVINQHTVEGPTMAQLLTRVLLKYVEGNVLMRTGNDITRRHKNL
                     LDSIKAYIDHNFYEPIRLDALATKFNVSPYYVSHEFKRRYGYSPMDYLIKRRLGEAQS
                     LLTTDEGGCEKIT"
BASE COUNT          238 a          148 c          194 g          243 t
ORIGIN      
        1 attctgtttt tttatattat aaaaaaatag ttttagtttt ggccgtagca ctacagattc
       61 ggataatgga ggaaaaatca tgcgtttttg cttctgtcct tcagaacaag ataataagtc
      121 ccgggctcag ttggcgtatg tcagtcaggt tagcgaaagc aatggcccgt ttgtacggtt
      181 catgcatatg catcccagga ccgtggagct aatccttatc accgagggaa agggggagta
      241 tttcatcggt gatcgcattt acccagtcag aaaaggtgat ttggttattt ataacagcga
      301 agtagtgcat gatgagtatc ttgaaagcgg acggcctgtg ggtactattt gctgtgggat
      361 aaacaatatt gtgtgcccgg gtctgcggga gaatgctctg attccagatg atactgtgcc
      421 cgtaattcca ctatatcatc attatgcgac gatcgagaaa ttgatgagtt caatatttaa
      481 tgttataaat cagcatacag tagaaggacc aacaatggcg cagttgctga ccagggtgct
      541 gcttaaatac gtcgagggaa atgtattaat gcgcacaggt aatgatataa cacggcgcca
      601 taaaaattta ctggattcaa taaaagctta tattgaccat aatttctatg aacccatccg
      661 tctggatgct ctggcgacta agtttaatgt cagcccttac tatgtttctc atgaatttaa
      721 acggcgctat ggatattcgc caatggacta tttgattaaa cgtcggttag gggaggcaca
      781 atctttactt accactgatg aaggtgggtg tgaaaaaatt acc
//
LOCUS       DAZGQL010000349          819 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.353, whole genome shotgun sequence.
ACCESSION   DAZGQL010000349 DAZGQL010000000
VERSION     DAZGQL010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..819
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.353"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>819
                     /locus_tag="ACHKIR_005390"
     CDS             <1..>819
                     /locus_tag="ACHKIR_005390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992960.1"
                     /translation="GNATRINGDSSVAIGLTSSVTGNDSVALGSHSSANGNNIVSVGS
                     DTLKRRITNLAQGKDDHDAVNLMQMTSAVNREAGERIKSTNNLQNQLNTLSGQVITET
                     RARTEGDAAALAAARAHSDENDKRTLVSANTYADQKETAINNRTDGLLTTERQARTAG
                     DTETLAAARTHSDENDKRTLVSANTYADQKETAINNRTDGLLMTERQARIAGDTATLA
                     AARTHSDENNKRTLVSANTYADQKETDINNRTDGLLMTERQARIAGDAETLATA"
BASE COUNT          266 a          218 c          201 g          134 t
ORIGIN      
        1 tcggtaatgc caccagaatt aacggtgatt catctgttgc cataggtctg acatcgtccg
       61 taaccgggaa cgactccgtg gcactgggca gtcattccag tgctaacgga aacaatatcg
      121 tatccgtcgg ctctgacaca ctgaaacgcc gcatcacaaa tctggcacag gggaaagatg
      181 accatgatgc ggtaaatctg atgcagatga ccagtgctgt taaccgggaa gctggagaaa
      241 gaataaaatc aacaaacaac ctgcagaatc agcttaatac cctctctggt caggtcataa
      301 ctgaaaccag ggcccgaaca gaaggtgatg cagcagcgct ggctgcagcc agagcccaca
      361 gcgatgaaaa cgacaaaaga acactggtca gtgcgaatac ctatgctgac cagaaagaaa
      421 ccgccatcaa taaccgtact gacggattac tgacgaccga acgccaggca cgcaccgcgg
      481 gcgatactga aactctggct gctgccagaa cgcacagcga tgaaaacgac aaaagaacac
      541 tggtcagtgc gaatacttat gctgaccaga aagaaaccgc catcaataac cgtactgacg
      601 gattactgat gaccgaacgc caggcacgca tcgcgggcga tactgcaact ctggctgctg
      661 ccagaacgca cagcgatgaa aacaacaaaa gaacactggt cagtgcgaat acctatgctg
      721 accagaaaga aaccgacatc aataaccgta ctgacggatt actgatgacc gaacgccagg
      781 cacgcatcgc aggtgatgca gaaactctgg caacagcag
//
LOCUS       DAZGQL010000350          815 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.354, whole genome shotgun sequence.
ACCESSION   DAZGQL010000350 DAZGQL010000000
VERSION     DAZGQL010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..815
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.354"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            348..>815
                     /locus_tag="ACHKIR_005391"
     CDS             348..>815
                     /locus_tag="ACHKIR_005391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992961.1"
                     /translation="MCDFIKSCYAAPRINKMADFFSSTASGVKHKPLKHVKIDNRLFP
                     SPEYIIFSDYLTKNSGPFVNHYFCSIPYSKEEECRLGVTIMKYASHRNKPLSLWCLGM
                     AEGAMARTISQLSKGQVLSLTTSPTKENEPVFFSHGSHIHSYFICSPFFLVHEE"
BASE COUNT          287 a          150 c          130 g          248 t
ORIGIN      
        1 ataatgcatt ctgagcatat cagagcgcat aaaaggcgtc aattatccat gctccctcac
       61 gaaacagaag ctgaactctt aacacctgct atcggaactc actgaataaa caactaaaaa
      121 ggacattgtg aaaattaaca tcaacagtat gtaaagtgat ttgttttgtc ttaaatgggt
      181 gaatgtaaaa aaatattcta tacaatccac acatccttgt atcatatatc aagtaaacat
      241 ccacagagat aacagcttat ttcaaataag cagattctgt ttgatacttc agaaaaatag
      301 tatatgctcc tgatttactg atgttataag ttatacagga tagttaaatg tgtgatttta
      361 taaaatcatg ttatgcagca ccaagaatta acaaaatggc tgattttttc tcatcaacag
      421 catctggagt aaaacataaa cctttgaagc atgtaaaaat tgataacagg cttttcccat
      481 ctcctgagta tataattttt tctgactatc ttacgaaaaa cagtggtcca tttgttaacc
      541 actacttttg cagtattcca tacagcaaag aagaagaatg caggctgggt gtgactatta
      601 tgaaatatgc aagccatcga aataagccat tgagcttatg gtgtcttgga atggcagaag
      661 gagccatggc cagaacaatt tctcaactat caaaaggaca ggttttatca ttaacaacca
      721 gcccaacaaa agaaaatgaa cctgtattct ttagtcatgg ttctcatatt cactcttact
      781 ttatttgctc cccattcttt cttgttcatg aagaa
//
LOCUS       DAZGQL010000351          796 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.355, whole genome shotgun sequence.
ACCESSION   DAZGQL010000351 DAZGQL010000000
VERSION     DAZGQL010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..796
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.355"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>796
                     /locus_tag="ACHKIR_005392"
     CDS             <1..>796
                     /locus_tag="ACHKIR_005392"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992962.1"
                     /translation="YLIQNEKIKVLAKSIAENGTSPLETLAVMENENGHFIALEGNRR
                     VCALTLLNDPDLCPENERAYFRKLSDNSDVIPDAVECVLFENREDANKWIELRHNGEQ
                     DGAGLLTWNATQKARHFGSSDNTLALKLLDYAETKGFISKQKRDGILTTASRFLGNPV
                     FRSTMGIRTGRSESNVMIDVSRDEFDKVLERFINDLINGENNVTSRSKKEDWENYAKQ
                     LRSEGIAPKGHCEPFALFSTEHSNASGKGSTISKIVKNNQSPNNRKY"
BASE COUNT          288 a          113 c          172 g          223 t
ORIGIN      
        1 ctatctgatt caaaatgaaa aaattaaagt attggctaaa agtattgctg agaatggaac
       61 aagtccatta gaaacacttg cggtgatgga gaacgaaaat ggacacttta ttgcccttga
      121 ggggaatagg agagtttgtg cattaactct gcttaatgat cctgatttat gtccagaaaa
      181 tgagagggct tatttcagaa aactatctga taatagtgac gtcattcctg atgcagttga
      241 atgcgtttta tttgaaaata gagaggatgc aaataaatgg attgaattgc gccataatgg
      301 agaacaagac ggtgcggggc ttcttacttg gaatgcaact caaaaagcaa gacatttcgg
      361 ttcttctgat aatacattag cgttaaaatt attagattat gctgaaacta aaggatttat
      421 ttcgaagcaa aaacgagatg gcattttgac aacagcatcg agattcttag gtaatcctgt
      481 attcaggtca actatgggaa ttagaacagg gcgttcagaa tctaatgtga tgattgatgt
      541 ttccagagat gaatttgata aagttcttga aaggttcatc aatgacttaa ttaatggtga
      601 aaataatgtc acatcaagat caaagaaaga agactgggag aattatgcaa aacagctaag
      661 atcggaaggt attgccccca aaggacactg tgaaccattt gctcttttta gtacggaaca
      721 tagcaatgct tctggtaagg gaagtactat aagtaaaata gttaaaaata accagagccc
      781 caataataga aaatat
//
LOCUS       DAZGQL010000352          795 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.356, whole genome shotgun sequence.
ACCESSION   DAZGQL010000352 DAZGQL010000000
VERSION     DAZGQL010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..795
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.356"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..327
                     /locus_tag="ACHKIR_005393"
     CDS             <1..327
                     /locus_tag="ACHKIR_005393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFO9992963.1"
                     /translation="VIPSKVKSRPQCTDDDALIISNCMAQLNVNNSDLHDFLYDYYVF
                     GMTLMGLGRKHERSDCWAGRVLQKAEGVIEGMLIIQGIKLEMDGYVERERSGAQASQF
                     SGRTGN"
     gene            527..682
                     /locus_tag="ACHKIR_005394"
     CDS             527..682
                     /locus_tag="ACHKIR_005394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO9992964.1"
                     /translation="MKQQKAMLIALIVICLTVILTVLVARKDLCEVRIRTGQTEVAVF
                     VDYESEK"
BASE COUNT          204 a          178 c          230 g          183 t
ORIGIN      
        1 gtgatcccat caaaggttaa atcacggcct cagtgtaccg atgatgatgc gctgattatc
       61 agcaattgca tggcgcaact gaacgtcaac aacagcgatt tgcatgattt tctgtatgat
      121 tattatgtgt tcgggatgac gcttatggga ctgggccgta agcatgagcg ctcagattgc
      181 tgggccgggc gggtactgca aaaagcagaa ggtgttattg aggggatgtt gattattcag
      241 ggaataaaac tggaaatgga cggatacgtt gagcgtgaac gatcaggggc acaggccagt
      301 cagttttccg gacgtacggg aaattgaaag cgcggggttt tactgtagaa tgactgcggg
      361 tgcttgaggg tgtctgcctc gggcacgccg ccgtaaggca gacggagaaa agccccagtt
      421 aacattacgc gtcctgcaag acgcttaaca ttaatctgag gccaatttca tgctgaacac
      481 atgcaggtta gcctcttacg tgccgaaagg caaggagaag caggctatga agcagcaaaa
      541 ggcgatgtta atcgccctga tcgtcatctg tttaaccgtc attctgacgg tactggtagc
      601 gaggaaagac ctctgcgagg tacgaatccg aaccggccag acggaggtcg ctgtcttcgt
      661 agactacgaa tctgagaagt aagagacctg gcgggggagt aatctcccgc cccctctgat
      721 gtgtcaggca tcctcaacgc acccgtactt tagttgtcat atgctccgca aaatgcaggt
      781 tgtcgttgca acccg
//
LOCUS       DAZGQL010000353          773 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.357, whole genome shotgun sequence.
ACCESSION   DAZGQL010000353 DAZGQL010000000
VERSION     DAZGQL010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..773
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.357"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..380
                     /locus_tag="ACHKIR_005395"
     CDS             <1..380
                     /locus_tag="ACHKIR_005395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992965.1"
                     /translation="LECSEYTLASSVVCRAFLENIYSLAYEKFYGNYPEGMAEHVVME
                     KIINKIEEDKNSLTKKEKKALEALKRVKTDTTNPLSPKTLGANAHAGFYPDPTALKVH
                     WDNISHILLFMLNKIFNSSSVHK"
     gene            432..>773
                     /locus_tag="ACHKIR_005396"
     CDS             432..>773
                     /locus_tag="ACHKIR_005396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HFO9992966.1"
                     /translation="MSATPSPLRYPGGKAAIQDMISAIICSNNLTGGHYAEPYAGGGG
                     LALSLLFKQYIHEIHLNDLDRSVWSFWHIILNNTEQFIDKIMTTPITIEEWHKQRYIQ
                     NNKDNYNDFELG"
BASE COUNT          280 a          141 c          140 g          212 t
ORIGIN      
        1 cacttgaatg ttcagaatac acattggctt cttcagttgt atgtagggct tttctggaaa
       61 acatctattc attagcatac gaaaaatttt atggaaacta tcctgaaggg atggctgaac
      121 atgtagtaat ggagaaaata ataaataaga tagaagaaga caagaatagc ctcactaaaa
      181 aagaaaagaa agctctggag gctctgaaaa gggttaaaac tgatacgacc aatccactat
      241 ctccaaaaac gctaggagca aatgcccatg caggcttcta cccagatcca acggcgttaa
      301 aagtccactg ggataacatt tcacatatct tattgtttat gttgaataaa atattcaaca
      361 gtagctctgt ccataagtaa tcaactgttc taaaataaat cggtatgaat ctattttcac
      421 agggaaagac tatgtcagca actccatcac cattacgata tccgggaggg aaagccgcaa
      481 ttcaagatat gatttcggct ataatttgct ccaacaacct tactggcgga cactatgcag
      541 aaccctacgc tggaggtggt ggtcttgctt tatcattatt attcaaacaa tatatacatg
      601 aaatacattt aaatgatctt gatcgctcag tttggtcatt ctggcatatc atacttaata
      661 acactgaaca gtttattgat aaaataatga cgacaccaat tactatagag gaatggcaca
      721 aacagaggta tatacaaaat aataaagaca actacaatga ttttgagctg ggt
//
LOCUS       DAZGQL010000354          767 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.358, whole genome shotgun sequence.
ACCESSION   DAZGQL010000354 DAZGQL010000000
VERSION     DAZGQL010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..767
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.358"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>767
                     /locus_tag="ACHKIR_005397"
     CDS             <1..>767
                     /locus_tag="ACHKIR_005397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004171002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HFO9992967.1"
                     /translation="EGIVITTVRSRGSRKSRFTLTFEGIPYSETVCWRPALLSRPVIS
                     GSLPARIESHEKNDIYAHLDDQGRYRVKLDFDRNSTERGYAYLWLRLAKPCAGDTYGF
                     HSPLIDGTEVAIVFDGGDPDRPYIAYALHDSEHPDHVTSDNHTRNVWRTPANNKLRME
                     DKRQEEHIKLATEYGKTQLNMGHLVNSRREKRGAGFELRTDEHGAVRAAKGLFLTADA
                     QARAQGPVLEMAAAISQINQANSQMQALNSAAEAAGAL"
BASE COUNT          179 a          207 c          236 g          145 t
ORIGIN      
        1 gaaggcatcg ttatcaccac ggtgcgttcc cgcggttcac gcaaaagtcg ctttacgctg
       61 acgtttgagg ggatccccta cagtgaaacg gtctgctggc gtccggcact gctcagtcgc
      121 ccggtgatat ccggctcgct tccggcgcgg attgagagtc acgagaagaa cgatatctac
      181 gctcatctgg acgatcaggg gcgttaccgg gtgaagctgg actttgaccg caacagtaca
      241 gaacggggtt acgcttatct gtggttgcgt ctggctaaac cctgtgccgg tgatacgtac
      301 ggtttccact caccgcttat cgacggaacg gaagtggcca ttgtatttga cggcggagat
      361 ccggatcgtc cctacatcgc ttatgccctg catgattcag agcatccgga tcatgtcacc
      421 agtgacaatc atacgcggaa cgtgtggcgc acgccggcga acaataagct gcggatggag
      481 gataagcgcc aggaagagca tatcaagctc gcaacggaat atggcaaaac acagctgaac
      541 atgggacacc tggtcaacag ccggcgtgaa aagcgaggtg ccggttttga actgcgaacg
      601 gatgagcatg gggctgtacg tgcagcaaaa gggctgttcc tgacggcaga tgcccaggcc
      661 agagcccagg ggccggtgct ggaaatggcg gccgcgataa gtcagattaa ccaggctaac
      721 agccagatgc aggccctgaa cagcgctgcc gaagccgccg gcgccct
//
LOCUS       DAZGQL010000355          766 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.359, whole genome shotgun sequence.
ACCESSION   DAZGQL010000355 DAZGQL010000000
VERSION     DAZGQL010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..766
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.359"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..241)
                     /locus_tag="ACHKIR_005398"
     CDS             complement(<1..241)
                     /locus_tag="ACHKIR_005398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992968.1"
                     /translation="MKVCLYFISVFFSSCCIADVFHYRMPFRIQTYSPVTKSKIENFA
                     IKDGAQKLLQINREKLLMLLNRSSNECYHFDDKNVR"
     gene            complement(299..>766)
                     /locus_tag="ACHKIR_005399"
     CDS             complement(299..>766)
                     /locus_tag="ACHKIR_005399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001600695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFO9992969.1"
                     /translation="KGGWNFYQYPLNPVSGFDPLGLKVSFQGDESTQKTLKEAYKAVA
                     ETKFGHKITEELESSEHEYIFRGLRKGINQTCYDDTEYSFYIDIDNDHSSCVYQGKNK
                     ACAMKPTLLSVVLAHEMGHAKGMKDDGTDSMANVDKYENPFRKELGLPARMKY"
BASE COUNT          238 a          156 c          101 g          271 t
ORIGIN      
        1 tcctaacatt tttatcatca aaatggtaac actcattact agacctgttc agtaacatta
       61 ataacttctc acgatttatc tgtaataatt tttgtgcacc atcttttatt gcaaaatttt
      121 caattttact tttagtgaca ggggaatatg tttggatgcg aaaaggcatc ctataatgga
      181 aaacatcagc tatacagcaa gatgaaaaaa atacggaaat aaaatacaga catactttca
      241 taccatctcc taaactggtg atatattaaa aacataccca aaagcaatat aaaaatgttt
      301 aatatttcat tcttgctggc aagcccaact ctttacgaaa tgggttttca tatttatcaa
      361 cattagccat cgagtcagtt ccatcgtctt tcattccctt tgcatgcccc atttcatgcg
      421 caagaaccac actcaacaga gtgggtttca tggcacatgc tttgtttttt ccttgatata
      481 cacatgagct atggtcgtta tcaatatcaa tgtaaaaact atattcagta tcatcgtagc
      541 atgtttggtt tatgcctttt cttaatcctc taaatatata ttcatgctct gatgactcta
      601 attcttctgt tattttatgt ccaaactttg tttctgctac agctttatat gcttctttta
      661 aagttttttg tgtactttca tctccctgaa aagacacttt taaccctaat ggatcgaaac
      721 cagataccgg attcaacgga tactgataaa aattccatcc cccctt
//
LOCUS       DAZGQL010000356          765 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.360, whole genome shotgun sequence.
ACCESSION   DAZGQL010000356 DAZGQL010000000
VERSION     DAZGQL010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..765
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.360"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..473
                     /locus_tag="ACHKIR_005400"
     CDS             <1..473
                     /locus_tag="ACHKIR_005400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Imm72 family immunity protein"
                     /protein_id="HFO9992970.1"
                     /translation="IIKDGISGFNNLGAYNYFIRGMNAPRQVYYDVLIESDTEMVVPD
                     DPIRYRDVHWRLVWEDTRYSDGIIPDESEYFLDDAPGKRITCPSGELCPHSGHWATIA
                     GGHQQFIDVQAGQLMPEATKYQSNMYADEIHLPAIWNLLRRDDEGSVYVKTGES"
     gene            551..>765
                     /locus_tag="ACHKIR_005401"
     CDS             551..>765
                     /locus_tag="ACHKIR_005401"
                     /inference="COORDINATES: protein motif:HMM:NF024894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4123 domain-containing protein"
                     /protein_id="HFO9992971.1"
                     /translation="MDGQRYFGESLSVQQPAAVPLFDTWPDSRIAFAGPWVMEMNSIT
                     DFRERLCELEAALPSVSWMISSSSLTEL"
BASE COUNT          194 a          162 c          197 g          212 t
ORIGIN      
        1 tgattattaa agatggtatc agcggattta ataacctcgg tgcttataat tattttattc
       61 gtggaatgaa tgctcctcgc caggtgtatt acgacgtatt aattgaaagt gacacagaaa
      121 tggtcgtacc cgatgaccca atccgttacc gggacgttca ctggcgtctg gtctgggaag
      181 acacgcgcta cagtgacgga attattcccg atgagtcaga atactttctg gatgatgcac
      241 caggcaaacg cattacctgc ccgagtgggg agctttgccc gcattcaggt cattgggcga
      301 ccatagccgg aggacatcag cagtttattg atgttcaggc cgggcagttg atgccagaag
      361 caactaaata tcagagcaat atgtatgcag atgaaatcca tcttcctgcg atatggaacc
      421 ttttacgccg ggatgatgag gggagtgttt atgtaaaaac aggggaaagt taaataactg
      481 atcaacgctt tttgacggac agatctgtta ccacctgatt tgctcaacaa aagtgacatt
      541 atttgctctg gtggatggtc agcggtattt cggtgagagc ctgtctgttc aacaacctgc
      601 cgccgtgccc ctgttcgata cctggcctga ttcgcgtatt gcatttgcgg ggccatgggt
      661 tatggagatg aacagtataa cggatttcag agaaagactg tgtgagcttg aagctgcgct
      721 tccgtcagtt tcctggatga tatcttcttc ctcgcttact gaact
//
LOCUS       DAZGQL010000357          759 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.361, whole genome shotgun sequence.
ACCESSION   DAZGQL010000357 DAZGQL010000000
VERSION     DAZGQL010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..759
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.361"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(147..656)
                     /locus_tag="ACHKIR_005402"
     CDS             complement(147..656)
                     /locus_tag="ACHKIR_005402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992972.1"
                     /translation="MQITEALLSEPGEIRRFVQQAVDHWPHLLAIHFTLHSTEGVCGQ
                     QIQTFCSSFHRRVHERITERDHTVSPSAPVVLRWLREQHEGAPIRCLLLLSQTSVCHP
                     RASVTVDEACSQVADLLQQTWPVISAGGQCRVERCFRVVRPDTSEQYVALKTAVQSLM
                     PRGIATIIR"
BASE COUNT          197 a          196 c          177 g          189 t
ORIGIN      
        1 actcattcag ttctgtaaag cagtatttat ccgccagata gtcaattcgt gtatgcatac
       61 tgaagagtat tccgtataaa gattcagctg gcaaaacttt atcagtctgt aaaaactaac
      121 ggaagagtcg atatttctcc cgtccatcac cggatgattg ttgcaatacc ccgtggcatc
      181 agagactgaa cagccgtttt taacgcaacg tattgctctg atgtatcagg ccggacaacc
      241 cgaaaacacc tttccacccg gcattgtccg ccagcgctta tcaccggcca ggtctgttgc
      301 agtaaatccg ccacttgcga acatgcttca tcaactgtga cactggcccg cggatggcaa
      361 acactcgtct ggctgagcag caacagacat cgtatcggtg ctccttcatg ttgttcccgc
      421 aaccagcgta ataccaccgg cgctgatggg ctgaccgtgt gatcacgctc cgtaatacgt
      481 tcgtgcaccc gtcggtgaaa ggaactacag aatgtctgaa tctgttgacc acagacgcct
      541 tctgtcgaat gaagtgtgaa gtggattgcc agcagatgcg gccagtgatc caccgcctgc
      601 tgaacaaaac gccggatttc ccccggctct gaaagtaagg cttcggttat ttgcactatt
      661 ttatctctgt tgaacttggt taagtcggtg cagacgcatc aacacaagta cggttcgatg
      721 caaacagctg tgactggcaa tatgaaagga atgatgaat
//
LOCUS       DAZGQL010000358          750 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.362, whole genome shotgun sequence.
ACCESSION   DAZGQL010000358 DAZGQL010000000
VERSION     DAZGQL010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..750
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.362"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>750
                     /locus_tag="ACHKIR_005403"
     CDS             <1..>750
                     /locus_tag="ACHKIR_005403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ImcF-related family protein"
                     /protein_id="HFO9992973.1"
                     /translation="LMLARPDKADAGWLAKNALKALPKRRNVPDGIWQNVAPKLLGFY
                     AQNLPAHPEWKIKPDDELISTVRQILLKQIGQRNAESGLYQEMLKRVASNWPDLTLAD
                     MTGDTDASTVFSTDEVVPGMFTRQAWEEQVQDAINEVVRTRRDEIDWVLTDKTHQTGS
                     DISPEALKIRLTERYFTDFGNAWLNMLNSIQWHEPTSLSGAIAQLSLLGDVRQSPLVA
                     LMNTLNYQGKTGQKGEALADSVVDSAKKLLD"
BASE COUNT          193 a          182 c          217 g          158 t
ORIGIN      
        1 atctgatgct ggcgcgcccg gacaaagcgg acgccggctg gctggcaaaa aatgcgctga
       61 aagccttacc gaaacgccgg aatgtgccgg atggcatatg gcagaacgtc gcaccgaaac
      121 tgctggggtt ttatgcgcaa aaccttccgg ctcatccgga atggaaaatt aagcctgacg
      181 atgaattaat cagtacggtc cgtcagattc tgcttaagca aattggccag cggaatgctg
      241 agtccgggtt atatcaggag atgcttaagc gcgtggccag taactggcct gatttaacac
      301 ttgcggatat gaccggtgac accgatgcct caacggtatt cagcactgac gaagtcgttc
      361 cgggaatgtt tacgcgtcag gcctgggaag agcaggtaca ggatgcgatt aacgaagtcg
      421 tcagaacacg ccgcgatgaa attgactggg tactgacgga taagacgcat cagacaggga
      481 gcgatatctc tccggaggcg ctgaagatcc gcctgactga gcgctatttt accgattttg
      541 gcaatgcctg gctgaacatg ctcaacagca tacagtggca tgaacccacg tcactttccg
      601 gagccattgc ccagttgagt ctgctcggtg atgtccgtca gtctccgtta gttgcactga
      661 tgaacacgct gaactaccag gggaaaacgg ggcagaaagg tgaggcactg gctgattcag
      721 tcgtggattc agcgaaaaag ctgctcgata
//
LOCUS       DAZGQL010000359          747 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.363, whole genome shotgun sequence.
ACCESSION   DAZGQL010000359 DAZGQL010000000
VERSION     DAZGQL010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..747
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.363"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..329)
                     /locus_tag="ACHKIR_005404"
     CDS             complement(<1..329)
                     /locus_tag="ACHKIR_005404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000197506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm57 family immunity protein"
                     /protein_id="HFO9992974.1"
                     /translation="MARLLTIVLFFSISANAVTVNQFQRELDLADKTILWALISASTQ
                     EGRKACSLSYFSCKAAEAELGLAYMAANDNKAFLTSLSRIMMYKIDAGLSESYTCYLL
                     SKGKIIRP"
BASE COUNT          206 a          155 c          126 g          260 t
ORIGIN      
        1 ggtcttatga tctttccctt gcttaataaa tagcatgtat aggattcgga aagtccagca
       61 tctattttgt acatcattat tctagataag gatgtgagaa atgccttgtt atcattagca
      121 gccatatatg ccagtccgag ttcagcctct gcggctttac atgaaaaata gctaagtgaa
      181 cacgcttttc gtccctcttg ggtactggct gatattagtg cccataatat tgtcttgtct
      241 gccaaatcca actctctctg aaattggttt acagtaactg cattcgcaga aatagagaaa
      301 aaaagcacaa ttgttaataa ccttgccata tttctagtcc gtcactgtta atgtgctatc
      361 tggctctgaa aatattagct catttaattt ttgcatgtca ttagatgaac cagttataca
      421 accctcggaa cagaatcgag gtcctaaaga tggaccaaaa gcgtttaaac aactatgtgt
      481 taaaaatcca tcacgattat acgtttctgt atttgctgac ggtactaaac gtataccatt
      541 aggttttcta ttggttgctc caggactgtt aagcaagcct cgtccggttt gcttcattga
      601 gttctccact ttagactatt ggactatctt tcaacatggc atcgccttag tataccgctg
      661 gttttccgct cattgttaat tttctctttc gttcatttca cttaattttc agttttttcc
      721 aaaagtcaat attcccttct aataaaa
//
LOCUS       DAZGQL010000360          745 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.364, whole genome shotgun sequence.
ACCESSION   DAZGQL010000360 DAZGQL010000000
VERSION     DAZGQL010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..745
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.364"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..242
                     /locus_tag="ACHKIR_005405"
     CDS             <1..242
                     /locus_tag="ACHKIR_005405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFO9992975.1"
                     /translation="EVSQYPELIAQFSSGNQTRIKQGLTAKAPLEGWRYGPKEIVKKF
                     QIHHRVAIEYGGSVYDIDNLRIVTPRLHDEIHYRR"
     gene            245..538
                     /locus_tag="ACHKIR_005406"
     CDS             245..538
                     /locus_tag="ACHKIR_005406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000471926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFO9992976.1"
                     /translation="MFKEKLEDYTEEEFLNFLGGLRSTMKDGKPLKGKELEMYWDSLV
                     DHFIEITQHPSGSDLIFYPKSQGDDKPENILKIVKEWRRSQGLPLFKDSKQSR"
BASE COUNT          257 a          125 c          159 g          204 t
ORIGIN      
        1 tggaggtgag tcagtatccg gagttgatag cgcagttttc aagtggtaac caaacacgta
       61 taaaacaagg attaaccgca aaagcacctc ttgaaggttg gcgttatggc cctaaagaaa
      121 tcgttaaaaa attccagata catcatcgtg ttgcaattga atatggcggc agcgtatacg
      181 atattgataa tttacgaatt gttaccccca gactacacga tgagattcac tacaggagat
      241 aaatatgttt aaagaaaagc tcgaagatta taccgaggag gaatttctta attttttagg
      301 cggattgaga tcaactatga aagatggaaa gcccttaaaa ggtaaggagc tagaaatgta
      361 ctgggattca ttagtagatc attttattga aattacacag catccgtcag ggagcgatct
      421 aattttctac cctaaaagtc aaggagatga taagcctgaa aacatcctga aaattgtcaa
      481 agaatggcgg cgttcacagg ggctaccctt gtttaaagac tctaaacaaa gtagataata
      541 aaactatata tggaacatca tcgcgtctgg ctaatgcgat gatgttaata acataaaata
      601 ataatgtggg tgctcttaaa aaacaggtaa aatactgaaa tctcattagg ttattttatc
      661 aatttcccgc tttcaccgat accctttggc tggaggtgag tcagtatccg gagttgatag
      721 cgcagttttc aagtggtaat caaac
//
LOCUS       DAZGQL010000361          736 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.365, whole genome shotgun sequence.
ACCESSION   DAZGQL010000361 DAZGQL010000000
VERSION     DAZGQL010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..736
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.365"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..420
                     /locus_tag="ACHKIR_005407"
     CDS             <1..420
                     /locus_tag="ACHKIR_005407"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFO9992977.1"
                     /translation="VGVDRTITAYKASRFGFEVKCYERLPYYCYGDLYKQLNFLLVTS
                     LYEGGPANIPEAIASGTPIISTPVGMVNDYVRDGINGLILTGDIDNDIDSINNFSQKS
                     KYQKLSSNALEMSGNIMSWQDVMQKTSLVYKELAGCA"
     gene            421..>736
                     /locus_tag="ACHKIR_005408"
     CDS             421..>736
                     /locus_tag="ACHKIR_005408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HFO9992978.1"
                     /translation="MKNISILFADSIHFHVRNFKSLFDLIDRHKIHHTFIHERNKWVS
                     AYGNYEEFFNELQPLYNKLLGKDIEQLYSLTIYGINIFKVCRDELLSYYLPIDSFRRL
                     LTG"
BASE COUNT          258 a           96 c          141 g          241 t
ORIGIN      
        1 gtaggagtcg atagaacaat aacagcatat aaagcatcaa gatttggttt tgaagtaaaa
       61 tgttatgaaa ggcttcccta ttattgttac ggtgatttat ataaacaact taattttctt
      121 ttagtaacga gtttgtacga aggtggacca gcaaatatac ctgaagcaat tgcatccggt
      181 acgccaataa tatcaactcc tgtgggtatg gtaaatgatt atgtcaggga tggtattaat
      241 ggtcttatat taactggaga tattgataat gatatcgact ctataaacaa tttctctcaa
      301 aaaagtaaat atcaaaaatt atcaagtaat gctcttgaaa tgtctgggaa cataatgtct
      361 tggcaggacg ttatgcaaaa aacatcattg gtatacaaag agttagcagg gtgcgcttga
      421 atgaaaaata taagtatatt atttgcggac agtatacatt ttcatgtgcg taattttaaa
      481 agtctattcg atttaatcga caggcataaa attcaccata cgttcataca tgaacgaaat
      541 aaatgggtga gtgcttatgg taattatgag gaattcttta atgaattaca gccattatat
      601 aataaattgc tggggaaaga tattgaacaa ttgtattcac ttacaattta tggaattaac
      661 atctttaaag tatgccggga tgaattatta tcgtattatt tgccaattga tagctttaga
      721 cggttgttaa ccggtg
//
LOCUS       DAZGQL010000362          734 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.366, whole genome shotgun sequence.
ACCESSION   DAZGQL010000362 DAZGQL010000000
VERSION     DAZGQL010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..734
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.366"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..305)
                     /locus_tag="ACHKIR_005409"
     CDS             complement(<1..305)
                     /locus_tag="ACHKIR_005409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992979.1"
                     /translation="MAKVKVKTLIGSVVTVAFFLTVIVAFLFYAFPENVASATGFGPY
                     DGLRILTFCAILVATLFLLGWLTEKAFDFAGKSGLYFQWGQKKAESITPVTNTIPGE"
     gene            complement(298..>734)
                     /locus_tag="ACHKIR_005410"
     CDS             complement(298..>734)
                     /locus_tag="ACHKIR_005410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992980.1"
                     /translation="SMVVLSNRNTHAWPDALCLHRPERGTETTLTKTLTRAFLWAKTR
                     PNELKGSWISGPTLTSGSSWNNACEENGVTFSLSEDNFSIDPVLGYTGHAAPWLAITL
                     ADAAFEQRGAQVIAAQPTADKDDVWVAVITKEEVRKESPGNG"
BASE COUNT          172 a          193 c          198 g          171 t
ORIGIN      
        1 tcttcaccgg gaatggtgtt cgtcaccgga gtgatactct cagccttttt ctgcccccac
       61 tggaaataca gcccggattt accggcgaag tcgaaagcct tctccgtcag ccagccgagt
      121 aaaaacagcg tggcaaccag tatggcgcag aatgtgagta tacgcagccc gtcatacgga
      181 ccgaagccgg tggccgaagc gacgttttcc ggaaaggcat aaaaaagaaa tgccacgatg
      241 accgtcagaa aaaaggcgac agtcacaaca gagccgatga gtgttttaac ttttacctta
      301 gccattcccg ggtgattcct tgcgaacttc ttctttagtg atgacggcaa cccagacgtc
      361 gtctttgtca gctgtgggct gggcggcgat aacctgcgca cctcgttgtt caaaggcagc
      421 atcagcaagc gtgatggcaa gccatggagc agcatggccg gtatagccca gcacgggatc
      481 gatactgaaa ttgtcttctg aaaggctgaa tgtcactccg ttctcttcgc aggcattatt
      541 ccagctgctg cccgacgtca atgtgggacc ggagatccag cttcctttaa gctcattggg
      601 tcgggtcttc gcccagagaa acgcccgggt aagtgtcttt gtgagcgttg tttctgttcc
      661 cctttcgggt cgatgaagac aaagcgcatc cggccaggca tgggtgttgc gattgcttaa
      721 taccaccatt gaga
//
LOCUS       DAZGQL010000363          733 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.367, whole genome shotgun sequence.
ACCESSION   DAZGQL010000363 DAZGQL010000000
VERSION     DAZGQL010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..733
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.367"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..264
                     /locus_tag="ACHKIR_005411"
     CDS             <1..264
                     /locus_tag="ACHKIR_005411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgM"
                     /protein_id="HFO9992981.1"
                     /translation="AQITAAAEDAEIHHDYARALILWQHAYHAATLTLNKNLAVAKID
                     FCIKRIRMHEQMSRIIRKTGTDEILFRLSKHHHLYEKKKTKEG"
     gene            complement(652..>733)
                     /locus_tag="ACHKIR_005412"
     CDS             complement(652..>733)
                     /locus_tag="ACHKIR_005412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_062863141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="subtilase cytotoxin subunit B"
                     /protein_id="HFO9992982.1"
                     /translation="LTSNALVGLSTCATSTECFGPDRKKN"
BASE COUNT          211 a          167 c          175 g          180 t
ORIGIN      
        1 gcgcagataa cggcagcagc tgaagatgcg gaaatacatc atgattatgc ccgggcatta
       61 atattgtggc agcatgcata tcatgctgcc acgctgactc tcaacaaaaa cctggcagtt
      121 gctaaaatcg acttctgtat taaacgtatc cggatgcatg aacaaatgag caggataatc
      181 cggaagactg gcacagacga aatactcttc cgtctgagta aacaccatca tttatatgag
      241 aaaaagaaaa caaaagaggg gtgagaaacc cattgtatgg ggaaaacttg ccatgctcac
      301 tgcgggggca tttttgtact ggctgttcta accgggtaac ccgttgatac acatagtaca
      361 ataatgtttt atgcaggctt acgcgccaga ctgccatcag tagtgcattg cgtgccagct
      421 cgcggacagg ggcagtgacc agccaccaca tgagtaagca tcatttcagc gccatcaggc
      481 agctcctgaa actcctgcga gttgaaaaag catcctcctg cattagcaat actggcttgt
      541 cagtgccagt gtgatataag acgtaatgtc aaagttcgtg gtgttgacat atttctgtcg
      601 caaccaaagt atgctcccgc ccagtatata tctgggcagg agcatcattt attagttctt
      661 tttcctgtcg gggccaaaac attctgtcga tgtggcgcag gttgataacc caacaagggc
      721 attggacgtt aat
//
LOCUS       DAZGQL010000364          725 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.368, whole genome shotgun sequence.
ACCESSION   DAZGQL010000364 DAZGQL010000000
VERSION     DAZGQL010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..725
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.368"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(52..285)
                     /locus_tag="ACHKIR_005413"
     CDS             complement(52..285)
                     /locus_tag="ACHKIR_005413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992983.1"
                     /translation="MARSDYDFINLSLEHELNEWLAERGYAGLADNRNRLAEVVACKL
                     QDSFCMNVSWDALNAAYSEHPEWFSGLVSGDEN"
     gene            complement(380..>725)
                     /locus_tag="ACHKIR_005414"
     CDS             complement(380..>725)
                     /locus_tag="ACHKIR_005414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO9992984.1"
                     /translation="FGCVYSLKASLSGVPDTYRYHLSHRIRRMVGSENTSLPYQQVAR
                     EVKAPRERLKYALEAGLLVTALDGLFWSGSQRIAADVLRLRKAGMPVVTTSVEVHDNL
                     TGTTRKIPAYHL"
BASE COUNT          175 a          225 c          158 g          167 t
ORIGIN      
        1 aagaaccccc tgccggcaat cacactgcct gatggcagca taatccactt attaattctc
       61 atccccggag acaagccctg aaaaccactc gggatgttca ctgtatgctg cgttcagagc
      121 atcccaggaa acgttcatac agaagctgtc ctgcaatttg caggcaacca cctctgccag
      181 gcggttccgg ttatccgcca gtccggcata acctctctct gccagccact cattcagttc
      241 atgttccaga gacagattga taaaatcgta atcggacctt gccattcccg tttccttaac
      301 acatgtcttc agactcaggg ataaagtata aaaatgtcgc cctgctgctg ccagagcaat
      361 attccccctg atggccgtat cagagatggt atgccggtat tttacgggtg gttcccgtca
      421 ggttatcgtg tacctccacg ctcgtagtca ccaccggcat tccggccttt ctcagcctca
      481 aaacatcagc tgcaatacgc tgactaccag accagaacag gccgtccagt gcagtcacca
      541 gtaaccccgc ctccagcgca tacttcagcc gttcacgggg cgctttcact tcccgggcaa
      601 cctgctgata tggcaatgat gtgttttcac tcccgaccat ccggcgaata cgatgagaca
      661 gatgataccg gtatgtatcc ggcacacctg aaaggctggc cttcaggctg tacacgcagc
      721 caaag
//
LOCUS       DAZGQL010000365          712 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.369, whole genome shotgun sequence.
ACCESSION   DAZGQL010000365 DAZGQL010000000
VERSION     DAZGQL010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..712
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.369"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..252
                     /locus_tag="ACHKIR_005415"
     CDS             <1..252
                     /locus_tag="ACHKIR_005415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992985.1"
                     /translation="SVWRMHTYEYPTATISCLTKLNNENILNIITGIEVILLAAKKPA
                     CKDRTDKIAEMLKIPFSVVKEFIDEQALLNKSNIQGTGS"
     gene            complement(299..451)
                     /locus_tag="ACHKIR_005416"
                     /pseudo
     CDS             complement(299..451)
                     /locus_tag="ACHKIR_005416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422102.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
BASE COUNT          241 a          153 c          139 g          179 t
ORIGIN      
        1 tccgtatgga gaatgcacac atacgaatat ccaacagcaa ccatcagttg tttgacaaaa
       61 ttaaataatg aaaatatatt aaatattatt acaggtatag aagtaatact cctcgcagca
      121 aaaaaacctg catgtaaaga cagaacagat aaaattgctg aaatgctgaa aataccattc
      181 tctgtcgtca aagaatttat tgatgaacag gctttattga acaaatcaaa cattcaggga
      241 actggcagtt gaacaaaacg ccagtccgaa gactggcgca gtgataaaac attctgcatc
      301 ataccacatt ctgctcctga agatgatgca gacgatgctc ccagtttccc gggtaatacc
      361 cgggcgtcag cagtacgcgg cgtcgctcat tacagagcga gttaccaatc actacttctc
      421 cggccacgcc acacagcgac agttgtatat aggggtttgg ggagcaatga aacagtaaac
      481 gccattaaga ggttaataaa aatagccaat tatttattaa tcaataaatt atatctattc
      541 attgaacgct ctcagctccc ccttactggg gtccgcctgg aattcagaaa ataaaccacg
      601 ttaagactgt tttttagcca aatgactttc agggatcgcc tgaatggcgg gacaacagcg
      661 gttttggtgt ccgaaatcgt acaattctgt acatctacac aagggaatta tc
//
LOCUS       DAZGQL010000366          709 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.370, whole genome shotgun sequence.
ACCESSION   DAZGQL010000366 DAZGQL010000000
VERSION     DAZGQL010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..709
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.370"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..113
                     /locus_tag="ACHKIR_005417"
     CDS             <1..113
                     /locus_tag="ACHKIR_005417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I restriction endonuclease subunit M"
                     /protein_id="HFO9992986.1"
                     /translation="IAELNAELKTTVSKIDQLRKDIDAIVAEIEGCEVQK"
     gene            110..>709
                     /locus_tag="ACHKIR_005418"
     CDS             110..>709
                     /locus_tag="ACHKIR_005418"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFO9992987.1"
                     /translation="MSELSYLEKLLDGAEVEWKTLGKVLKRTKGTKITAGQMKALHKD
                     NAPLKIFAGGKTVAFVDFKDIPEKDINREPSIIVKSRGIIEFEYYDKPFSHKNEMWSY
                     HSNNDAISIKYIYYFLKINEGYFQKIGGKMQMPQIATPDTDKFEVPIPCPDNPEKSLA
                     IQSEIVRILDKFSALTAELTAELNMRKKQYNHYLDKLLNF"
BASE COUNT          255 a          126 c          153 g          175 t
ORIGIN      
        1 atattgctga gctgaatgct gagctgaaaa ccacggtcag caaaatcgac cagttgcgta
       61 aagatattga tgcgattgtg gctgaaattg aaggctgcga ggtgcagaaa tgagcgagtt
      121 gagttatctg gaaaaattgc tggatggggc tgaggttgag tggaaaacac tagggaaagt
      181 gcttaaacga actaaaggca caaaaataac cgctgggcag atgaaagctc ttcacaaaga
      241 taatgcgccg cttaaaattt ttgcaggagg aaaaacagta gcctttgttg actttaaaga
      301 tataccagag aaagatatta atagagagcc atcgattatt gttaaatcca gaggtattat
      361 cgaattcgag tattacgata aaccattctc tcacaaaaat gagatgtggt catatcactc
      421 aaataatgat gcgataagca taaagtatat ttattacttc ctaaaaataa atgaaggata
      481 tttccagaaa attgggggta aaatgcaaat gccacaaatt gcaactcctg atacagataa
      541 gtttgaagtt ccaatcccct gcccggacaa tccggaaaaa tcccttgcca tccagtctga
      601 aatcgtgcga attctggaca aattcagcgc cctgacagca gagctgacag cagagcttaa
      661 catgcgtaaa aaacaataca accactatct agataagtta ttgaatttc
//
LOCUS       DAZGQL010000367          699 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.371, whole genome shotgun sequence.
ACCESSION   DAZGQL010000367 DAZGQL010000000
VERSION     DAZGQL010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..699
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.371"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>699
                     /locus_tag="ACHKIR_005419"
     CDS             <1..>699
                     /locus_tag="ACHKIR_005419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000431134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO9992988.1"
                     /translation="LTLSHSQLQRLSPLLDAQSDECPERLQDAEPVLPQGDNVTFIGR
                     PVGADTAGIPSGDLPPVLQRALDKFTRDITASAREGKIDPVSGRDTEIRQMVDILSRR
                     RKNNPILVGDPGVGKTALVEGLALRIAEGNVPESLRPVTLRTLDLGLLQAGAGVKGEF
                     EQRLKNVIDAVQQSPAPVLLFIDEAHTLIGAGNQAGGADAANLLKPALARGELRTIAA
                     TTWSEYKQYLERDAA"
BASE COUNT          142 a          197 c          224 g          136 t
ORIGIN      
        1 gctgacactg agtcatagcc agttgcagcg tctgtccccg ctgctggatg cgcagtctga
       61 tgagtgtccg gagagattac aggatgcaga gcctgtgctg cctcagggag acaatgtgac
      121 ctttatcggg cgtcccgtcg gtgcggatac ggcgggtata ccgtcaggtg acctgccgcc
      181 tgtgttacag cgtgctctgg ataaattcac ccgggacatc acggccagcg cgagagaggg
      241 gaaaattgac ccggtatcgg gacgcgatac ggaaatccgt cagatggtgg atattctctc
      301 ccgccgccgc aagaacaacc cgattctggt gggggacccc ggtgtgggga aaacggctct
      361 ggtggaaggg ctcgccctgc gtattgcgga aggtaacgta ccggaatcac tcagacccgt
      421 caccctgcgc acccttgacc tcggcctgct gcaggccggt gccggcgtga agggtgaatt
      481 tgaacagcgc ctgaaaaacg tgattgatgc cgtgcagcag tcaccggctc ctgtactgtt
      541 gtttatagat gaagcccata cccttatcgg ggccggtaat caggccggcg gcgcggatgc
      601 ggccaacctg ctgaagcctg cgctggcgcg gggtgaactg cgtaccattg ccgccaccac
      661 ctggtctgag tacaaacaat acctggaacg tgacgcggc
//
LOCUS       DAZGQL010000368          693 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.372, whole genome shotgun sequence.
ACCESSION   DAZGQL010000368 DAZGQL010000000
VERSION     DAZGQL010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..693
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.372"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            47..>693
                     /locus_tag="ACHKIR_005420"
     CDS             47..>693
                     /locus_tag="ACHKIR_005420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001599750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992989.1"
                     /translation="MIRYSGIFLSLLMLSGCATEALKNEQAETVITAGNKAVAANASF
                     IKQYYASRENFLVNFYATNPDCALRPDYTIDIGGDGNGGKLCQRSGPNTKQVQLVPFE
                     KEKLLASMEVINSMSAYVELLTKDMATTESQQVALLKQALDHANNAQTLLGVSHSKDL
                     AQSAAAVKDLVNYFNELYIIKVNSGKIGKTVDENWVRLIKNMTVLRNDIYTKGDK"
BASE COUNT          241 a          115 c          145 g          192 t
ORIGIN      
        1 ttactattta attatctgag ataaactcaa cgtcaagagg tgggttatga ttagatatag
       61 tggaatattt ctatcgctgt taatgctttc aggttgtgca actgaagcat tgaaaaacga
      121 acaagctgaa actgttatta ctgcgggtaa caaagcagta gcggcgaatg caagtttcat
      181 taaacaatac tatgcttctc gtgaaaactt ccttgttaat ttctacgcga ctaatccgga
      241 ttgtgccctg cgacctgatt ataccattga tataggcggt gatgggaatg gcggaaaatt
      301 atgccagcgt tcagggccaa atacaaaaca agtacaatta gtccctttcg agaaagaaaa
      361 attactggcc tcaatggaag tcattaatag catgagcgct tatgtggaac ttctgacaaa
      421 ggatatggca actacggaaa gtcagcaagt ggcattactt aaacaggctc tggatcacgc
      481 aaacaacgca caaacactac ttggtgtttc acatagcaaa gatttagctc aatctgcagc
      541 agcggttaaa gatttggtaa attatttcaa tgagttatat ataattaaag tcaattctgg
      601 aaaaataggc aaaacggtag atgaaaactg ggtaagactt ataaaaaaca tgactgtttt
      661 gcgtaatgat atttatacaa aaggcgataa gat
//
LOCUS       DAZGQL010000369          679 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.373, whole genome shotgun sequence.
ACCESSION   DAZGQL010000369 DAZGQL010000000
VERSION     DAZGQL010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..679
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.373"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..617)
                     /locus_tag="ACHKIR_005421"
     CDS             complement(<1..617)
                     /locus_tag="ACHKIR_005421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HFO9992990.1"
                     /translation="MNIRNNHLPGTETLFSETETANSKQLAVLKTNFPQCFDKNGAFI
                     QEKLLEIIRASNVEISKESYCLNWLGKSYARLLANLPPTTLLSEDKDHNRREENKNSQ
                     NLLIKGDNLEVLKHMVNAYSEKVKMIYIDPPYNTGSDGFAYNDDRKFTPEQLSDLAGI
                     DLDEAKRILEFTTNGSSSHSAWLTFIYPRLYIARELLREDGVIFIS"
BASE COUNT          197 a          133 c          140 g          209 t
ORIGIN      
        1 gaaataaata tcacaccatc ttcacgtaga agctcacgag caatatataa acgaggataa
       61 atgaacgtta accatgcgct atggctgctt gaaccattcg tggtaaattc aagtatacgt
      121 ttagcctcat caagatcgat acctgccaga tcagaaagct gttctggtgt aaatttgcga
      181 tcatcattat aggcgaaccc atcagaaccc gtgttatatg gcggatcgat ataaatcatt
      241 ttaacctttt cagaataagc attaaccata tgctttaata cctcaaggtt atcccctttg
      301 attaacagat tttgactgtt cttgttctct tcacgtctgt tatggtcttt atcttctgac
      361 agcaatgtcg ttggtggtag gttagccagc aggcgagcat aggatttgcc aagccagttt
      421 aagcagtatg actctttaga gatttcaaca tttgatgctc tgatgatctc aagcagcttt
      481 tcctgaatga aagctccgtt tttatcgaaa cactgtggaa agttagtttt caaaacagca
      541 agttgtttac tgttcgcagt ttcagtttca gaaaaaagag tttctgttcc cggtagatga
      601 ttgttgcgta tattcataca gcacctccag aaagcacgga ggtagaggta cttgacggag
      661 agagttcagg cgagcatat
//
LOCUS       DAZGQL010000370          679 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.374, whole genome shotgun sequence.
ACCESSION   DAZGQL010000370 DAZGQL010000000
VERSION     DAZGQL010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..679
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.374"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>679
                     /locus_tag="ACHKIR_005422"
     CDS             <1..>679
                     /locus_tag="ACHKIR_005422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system domain-containing
                     protein"
                     /protein_id="HFO9992991.1"
                     /translation="GSAVADWPEAEKPSFAGLCSALENRLARSGGMEALVPQNSSPRE
                     TGRESAPPDSPQLAAVTSGRDLLDQARLLSRWLSGQPQGWLASHRLMKTVRWDTVDQL
                     PSLDSSGRTRLVPPKTGYRAQLKRLWLQKNWVELIELASQMYCEGVNHFWLDLQWYLW
                     QGLSHAGQPWDSWSDAVLSDLRLLLKRLPGLEGLAWNDGTPFADEVTLAWIAEKVNEE
                     DVMYGDEA"
BASE COUNT          128 a          190 c          218 g          143 t
ORIGIN      
        1 ggatccgccg tggcagactg gccggaagcg gagaagccgt cttttgcggg gctttgttcc
       61 gcgcttgaga accgcctggc acgttcaggg ggcatggagg cactggtccc ccagaacagc
      121 agtccccggg agaccgggcg tgaatcagct cccccggact caccacaact ggcagcggtg
      181 acctcggggc gcgacttact cgaccaggcc aggttacttt cccgctggct gagcgggcag
      241 ccgcagggct ggctggcttc tcaccggctg atgaagacgg tgcggtggga tacggtcgat
      301 cagctccctt cgctggacag tagcggacgc acccgcctgg tccccccgaa aaccgggtat
      361 cgcgctcagc ttaagcgact ctggcttcag aaaaactggg ttgagctgat tgaactggcc
      421 agtcagatgt attgcgaagg tgtgaatcat ttctggctcg atcttcagtg gtatctctgg
      481 cagggactca gtcatgctgg tcagccctgg gattcctggt ctgacgctgt cctgtcagat
      541 ctgcggctgt tgcttaaacg tctcccgggc cttgaaggac tggcatggaa tgacggtact
      601 ccctttgctg acgaggtcac cctcgcctgg atcgctgaaa aagtgaatga agaagatgtg
      661 atgtacggcg atgaagctg
//
LOCUS       DAZGQL010000371          674 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.375, whole genome shotgun sequence.
ACCESSION   DAZGQL010000371 DAZGQL010000000
VERSION     DAZGQL010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..674
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.375"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            219..>674
                     /locus_tag="ACHKIR_005423"
     CDS             219..>674
                     /locus_tag="ACHKIR_005423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9992992.1"
                     /translation="MGKGGGKGHTPREAKDNLKSTQMMSVIDAIGEGPVEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGAETALGVEVTKAKPVTR
                     TITSANIDRLRVTFGVQSLVETTSKGDRNPSSVRLLIQLERNGHWVTEKD"
BASE COUNT          165 a          163 c          217 g          129 t
ORIGIN      
        1 taacgggagg aacgaatagt caccttaagg agttgcgatt aattgctttt attgaccgca
       61 ggtaatctac ccatccggca aggtttcctc cacgggaatt tgccggaaat gtgaagaatt
      121 tcagaaattt tattccgtca tgacacaggc accctccggg gtgcctgtcg ttttctggca
      181 taaacagatt cagacatcag acaggagagg gggacagagt gggtaaaggg ggcggcaagg
      241 ggcacacacc gcgtgaggcg aaggacaatc tcaaatccac gcagatgatg agcgtgattg
      301 atgccattgg tgagggaccg gtggaaggtc cggtgaaggg actgcagagt attctggtga
      361 acaaaacccc gctgacggac acggacggta atcccgtgat acacggtgtg accgccgtct
      421 ggcgtgccgg ggagcaggag cagacaccgc cggaaggctt tgagtcctcc ggggcagaaa
      481 ccgcactggg cgtggaggtg acgaaggcaa agccggtgac gcgcaccatc acgtcagcga
      541 acattgaccg tctgcgggtc accttcgggg tgcagtcact ggtggagacc acctcaaagg
      601 gtgaccgtaa tccctcttct gtccgcctgc tgattcagct tgagcgtaac gggcactggg
      661 tgacggagaa ggat
//
LOCUS       DAZGQL010000372          671 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.376, whole genome shotgun sequence.
ACCESSION   DAZGQL010000372 DAZGQL010000000
VERSION     DAZGQL010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..671
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.376"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(89..547)
                     /gene="tnpA"
                     /locus_tag="ACHKIR_005424"
     CDS             complement(89..547)
                     /gene="tnpA"
                     /locus_tag="ACHKIR_005424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element IS200C family
                     transposase"
                     /protein_id="HFO9992993.1"
                     /translation="MGNEKSLAHTRWNCKYHIVFAPKYRRQVFYREKRRAIGSILRKL
                     CEWKSVRILEAECCADHIHMLVEIPPKMSVSGFMGYLKGKSSLMLYEQFGDLKFKYRN
                     REFWCRGYYVDTVGKNTAKIQDYIKHQLEEDKMGEQLSIPYPGSPFTGRK"
BASE COUNT          157 a          187 c          125 g          202 t
ORIGIN      
        1 ccggaggttt ttcaaatgcg cctataaggc tctcttacca gccgcgccct aacaggcgca
       61 cacgatctga catttgcatc caacttcgtt acttacggcc cgtaaacggg ctgcccggat
      121 agggaatcga taactgctct cccattttat cctcttcaag ctggtgcttt atgtaatcct
      181 gtatcttcgc cgtgttctta cccaccgtat cgacgtagta ccctctgcac cagaactctc
      241 tgttcctgta tttgaatttc aaatcaccaa actgctcgta aagcatcaga ctgcttttcc
      301 ctttcagata tcccataaag ccggatacac tcattttggg cgggatctcc acaagcatat
      361 ggatatgatc tgcacagcat tcagcttcaa gaatccgtac gcttttccac tcacacagct
      421 ttctcaaaat actgcctatt gctctacgct tctctctgta gaacacttgt cttcggtatt
      481 ttggcgcaaa aactatgtga tatttacagt tccatcgggt gtgcgctaag ctcttttcgt
      541 tccccattgg gacccccttt tgatttcttg tttgacactt gcagttgcca gaccgcaagg
      601 tgttttaaca aatcaaaagg ggttttaata actggctcaa agctgaaagc tttccggaac
      661 ccccagccta g
//
LOCUS       DAZGQL010000373          660 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.377, whole genome shotgun sequence.
ACCESSION   DAZGQL010000373 DAZGQL010000000
VERSION     DAZGQL010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..660
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.377"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..249
                     /locus_tag="ACHKIR_005425"
     CDS             <1..249
                     /locus_tag="ACHKIR_005425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tox-REase-5 domain-containing protein"
                     /protein_id="HFO9992994.1"
                     /translation="KDKLCQFWEAKARYDQFFRADGSKKPWWTGNLSAINQASRHQAV
                     ATVNQPLKVVWIFMQPVSCEYFRKMFKDYKDIITRWQP"
     gene            246..>660
                     /locus_tag="ACHKIR_005426"
     CDS             246..>660
                     /locus_tag="ACHKIR_005426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015386758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992995.1"
                     /translation="MIELFNTSINIHSLKERDITIDDCLNELYRSTVVVKNLTQHDHI
                     WYLTGYSRKDASKHVVFSDNEPNQNTIDTFERHYKKNRPLINESIWNGQPDGLACSIS
                     HFMKFIDNPKKLNLIIDIDQRVANTSDMIDSLLLLA"
BASE COUNT          226 a          110 c          134 g          190 t
ORIGIN      
        1 aaagataagc tgtgtcagtt ctgggaagct aaagcgcggt atgaccaatt tttcagggct
       61 gatggttcaa agaaaccctg gtggacaggt aatctaagcg caattaatca agcatcccga
      121 catcaggcgg ttgcaacagt caatcagcca ttgaaagttg tctggatatt tatgcaacct
      181 gtaagttgtg agtatttcag gaaaatgttt aaagattaca aggatattat tacgaggtgg
      241 caaccgtgat tgaattattt aacacgtcaa taaatataca ttctttgaaa gaaagagaca
      301 taacgataga cgattgtttg aatgaactat acagaagtac cgtagttgtt aaaaatctaa
      361 cgcagcacga tcacatatgg tacttaactg gctactcaag aaaggacgct tcaaagcatg
      421 tcgtgtttag tgataatgaa ccgaaccaaa atacaataga tacctttgag cggcattata
      481 aaaagaatcg tcctttaatt aatgaatcta tatggaatgg acaacccgat gggctggctt
      541 gctcaatatc acattttatg aaatttattg ataaccctaa aaaactaaat cttatcatag
      601 atattgatca gagagttgcc aatacttcgg atatgattga ttccttgctt ctgttggctg
//
LOCUS       DAZGQL010000374          656 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.378, whole genome shotgun sequence.
ACCESSION   DAZGQL010000374 DAZGQL010000000
VERSION     DAZGQL010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.378"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(455..>656)
                     /locus_tag="ACHKIR_005427"
     CDS             complement(455..>656)
                     /locus_tag="ACHKIR_005427"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9992996.1"
                     /translation="TAPFITGNTQQWLCDPYPSGDAGMFLLRNGGIDFFYSFHLFAST
                     SCDCARCIRPFEIWSLTRVSGV"
BASE COUNT          198 a          164 c          147 g          147 t
ORIGIN      
        1 aacacaacag aaaaactcat ctgcatttct ccctgacgac acggttaaac gttgcgatcc
       61 ttcgggtagt gactgcctgt gcgccagggt agcgcagatt gtttaggtat tgaggggtaa
      121 caaatcggtg ttgcacaccg actgcagcat ttgctgtacc tgtctccagg tcgcccccga
      181 caccacctct aataagtggt atcgccgatt cgacatctca ctacataaaa ctccttaaaa
      241 aacaattgat tctgcaaaaa accatttatg gacgttattc acaaaatcgt ccgtaaattt
      301 gcagaagagg ttgaaaaaac actctcacgc cgggtcactt cttcaaatgt taaaaaacat
      361 cacgtgaaac ggcagttcgt gcgggttcgt taagcagacg cgcgtgcgtc actttgtcat
      421 cgtgacacgc tgacgaaagt gacgccgcgc agcttcagac gccagataca cgcgtaagcg
      481 accaaatttc aaatggtctt atacagcgcg cacagtcgca ggaagtgctg gcaaacaaat
      541 gaaaagaata aaagaagtca ataccgccat tacgcagtaa gaacattccg gcatcaccgg
      601 atggataagg atcacacaac cactgctgtg tattgcccgt aataaagggc gcggtt
//
LOCUS       DAZGQL010000375          640 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.379, whole genome shotgun sequence.
ACCESSION   DAZGQL010000375 DAZGQL010000000
VERSION     DAZGQL010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..640
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.379"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..>640)
                     /locus_tag="ACHKIR_005428"
     CDS             complement(<1..>640)
                     /locus_tag="ACHKIR_005428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2345 domain-containing protein"
                     /protein_id="HFO9992997.1"
                     /translation="LENAAERAQALKADIDSQIQMFEQRLKLLNEAVLFSAPEGMALT
                     SGEDMQMSAARNVAVNAGGDISTGTMGNLTALAGEKLGLFARTGQLSLKSGEGAVDVQ
                     AQNGSMRLFAEKKLTISSESDISFTGKKRITLIGGGSYLRLEAGKVEYGTMATCMRRV
                     KRTMTEAAQSMPMTFPRMSSSHIYSAIYRLLDKHGNILANTPCRLTTPSGQTV"
BASE COUNT          131 a          201 c          151 g          157 t
ORIGIN      
        1 ttactgtctg accagatgga gtggtgaggc gacagggggt attggccaga atattgccgt
       61 gtttgtccag caaccgataa atagccgagt agatatgcga agaactcata cgggggaatg
      121 tcatcggcat actctgcgca gcctccgtca tcgtccgctt cacccgacgc atacaggttg
      181 ccatcgtacc atactcgacc ttcccggctt caagccgcag gtaactcccc ccgccaatca
      241 gcgtgatgcg tttcttgccg gtaaacgaga tatcactttc tgacgaaatg gtcagcttct
      301 tctcagcaaa caaccgcatg ctgccgtttt gcgcctgaac atccactgcc ccttccccgg
      361 atttcaggct aagttgaccg gtccgggcaa aaagccccag cttctctccg gccagcgcgg
      421 tcaggttgcc cattgttccg gtactgatat cccccccggc gttgacagcc acatttcgtg
      481 ctgcactcat ctgcatatct tcaccgctgg tcagcgccat tccttccggt gccgagaaaa
      541 gcactgcttc attgagcagt ttcagccgct gctcaaacat ctgtatctga ctgtcaatat
      601 cagccttcag cgcctgtgcc cgttctgcgg cattttccag
//
LOCUS       DAZGQL010000376          628 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.380, whole genome shotgun sequence.
ACCESSION   DAZGQL010000376 DAZGQL010000000
VERSION     DAZGQL010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..628
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.380"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..588
                     /locus_tag="ACHKIR_005429"
     CDS             <1..588
                     /locus_tag="ACHKIR_005429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFO9992998.1"
                     /translation="QGTRHSMKASSGNNDFRARGWGWLGSLETGLPFSITDNLMLEPR
                     LQYTWQGLSLDDGKDNAGYVKFGHGSAQHVRAGFRLGSHNDMTFGEGTSSRAPLRDSA
                     KHSVRELPVNWWVQPSVIRTFSSRGDMRVGTSTAGSGMTFSPSQNGTSLDLQAGLEAR
                     VRENITLGVQAGYAHSVSGSSAEGYNGQATLNVTF"
BASE COUNT          129 a          186 c          189 g          124 t
ORIGIN      
        1 cagggaaccc gccacagtat gaaagcgtca tcgggcaata acgacttccg cgcacggggc
       61 tggggctggc tgggctcact ggaaaccggt ctgcccttca gtatcactga caacctgatg
      121 ctggagccac ggctgcagta cacctggcag gggctttcgc tggatgacgg taaggacaac
      181 gccggttatg tgaagttcgg gcatggcagt gcacagcatg tgcgtgccgg tttccgtctg
      241 ggcagccaca acgatatgac ctttggtgaa ggcacctcat cccgtgcccc cctgcgtgac
      301 agtgcaaaac acagtgtgcg tgaactgccg gtgaactggt gggtacagcc ttccgttatc
      361 cgcaccttca gctcccgggg agatatgcgt gtggggactt ccactgcagg cagcgggatg
      421 acgttctctc cctcacagaa tggcacatca ctggacctgc aggccggact ggaagcccgt
      481 gtccgggaaa atatcaccct gggcgttcag gccggttatg cccacagcgt cagcggcagc
      541 agcgctgaag gctataacgg tcaggccacg ctgaatgtga ctttctgata atcaggtcac
      601 tccccttcag gccccggtca tcatgacc
//
LOCUS       DAZGQL010000377          620 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.381, whole genome shotgun sequence.
ACCESSION   DAZGQL010000377 DAZGQL010000000
VERSION     DAZGQL010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..620
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.381"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(429..>620)
                     /locus_tag="ACHKIR_005430"
     CDS             complement(429..>620)
                     /locus_tag="ACHKIR_005430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncH family protein"
                     /protein_id="HFO9992999.1"
                     /translation="ITLPFIQLVYFISSEKKLTIHIVQMFQLLSQAFYNLKMFLMMDM
                     LGVGDAININTNKNIRQVC"
BASE COUNT          203 a          123 c           99 g          195 t
ORIGIN      
        1 cacattgcca tcttaacgcc gttaaacact ggcttaatag tggatattta aaattaggaa
       61 tttattaaat tcacctgatg aatatcataa gcaaatagca tagaaaataa tgacaactcc
      121 cccgagggga ccatttccaa agcactgcac caccacctgg tgcccggatg atcttgcttc
      181 agtggtaaga gtacctgatt cattgttccc aaaatggtgt tttcccctgc ccctccaata
      241 atgtgcagct ctcacgtgat aatctattga ttaggttata ttaagaatca atatttgaaa
      301 tacatccatt ttcttgaata aatcaacatg tttatttatc aattgctgtt ttagctctga
      361 ctcatcaagc gctccatatt tgtcctacag ataatcgcga ataaatacag agaacgccat
      421 ttatcctttt agcatacctg ccggatattt ttatttgtat taatattaat tgcatctcca
      481 actccgagca tatccatcat taaaaacatt ttgagattat agaatgcctg tgataacaac
      541 tgaaacatct gcacaatgtg aatagttagt ttcttttcag aggaaatgaa ataaacaagc
      601 tgaatgaaag ggagagtgat
//
LOCUS       DAZGQL010000378          615 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.382, whole genome shotgun sequence.
ACCESSION   DAZGQL010000378 DAZGQL010000000
VERSION     DAZGQL010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..615
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.382"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..221
                     /locus_tag="ACHKIR_005431"
     CDS             <1..221
                     /locus_tag="ACHKIR_005431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015386758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9993000.1"
                     /translation="GWMLYMPHILLPELIPEAAKIVPVFEGEKQKGTIVVSTEDVFDG
                     NNKEHIGKANDIEIRLLDLGLLPLLTEL"
     gene            476..>615
                     /locus_tag="ACHKIR_005432"
     CDS             476..>615
                     /locus_tag="ACHKIR_005432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000018100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFO9993001.1"
                     /translation="MNRKSPSLYEILYGNFTGGLELNQVSEEEQVILSVLDNMQRILN
                     TRA"
BASE COUNT          203 a          105 c          126 g          181 t
ORIGIN      
        1 tgggatggat gttatatatg ccccacatac tcttacctga attaatccct gaagccgcaa
       61 aaatagtacc ggttttcgag ggcgagaagc aaaagggaac tattgttgtt tcaaccgaag
      121 acgtatttga cggaaataat aaggaacaca tcggaaaagc taatgatatt gaaataagac
      181 ttctggatct tggactgcta cctttgctaa cagagcttta atccaacctt atcactcatg
      241 ggtagatgac gggacagtta cacccgttaa tggccttatt tttaacacat cagataaaat
      301 tgaatgctca ccattaagaa tacctggaga aattaaaaaa cggagattgt ttcaagagct
      361 tcttttattt aagggtttac aggtcaaaaa tgtgaataga ctagtatcat gactgttgta
      421 tagctgtatt aaattagata tttaacatga cagtaataca aattggatta ttattatgaa
      481 ccgaaaatcc ccatcattat atgaaattct ctatggtaat ttcactggcg ggctggagtt
      541 aaatcaggtc agtgaggaag agcaggttat tctttcggtg ctcgataaca tgcagcgtat
      601 tctgaatacc cgggc
//
LOCUS       DAZGQL010000379          600 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.383, whole genome shotgun sequence.
ACCESSION   DAZGQL010000379 DAZGQL010000000
VERSION     DAZGQL010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..600
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.383"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>600
                     /locus_tag="ACHKIR_005433"
     CDS             <1..>600
                     /locus_tag="ACHKIR_005433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001499016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HFO9993002.1"
                     /translation="FINGTVCTCTVYGDTQYFHWRVDYSTKNKPDTVSQRDASTTQKG
                     VVQLSSDTGSNDETKAATPKAVKAAMDVANEAKTKAEEAAAGGGVPGPKGEKGDPGAQ
                     GPKGETGATGPAGPRGAQGPKGEKGDPGPRGERGETGPMGPPGTSDGKSRVVGIRLGN
                     KQTYAPQTDSTTWAVDLDIGAMITGIGGYNDGNKTLIDRV"
BASE COUNT          172 a          130 c          208 g           90 t
ORIGIN      
        1 gtttattaat ggcacggtct gtacctgtac ggtttacggc gacacacagt atttccactg
       61 gcgggtggat tacagcacaa aaaacaaacc ggatacggtc agccagcggg atgccagcac
      121 gacgcagaaa ggtgtggtgc agttaagcag tgatactggc agtaatgacg aaacaaaggc
      181 agccacgccg aaggccgtga aggcggcaat ggatgtggca aatgaagcga aaacaaaggc
      241 agaagaggct gcagcaggag gtggtgttcc cggtccgaaa ggagagaaag gcgaccccgg
      301 agcacagggt ccgaaaggtg aaacgggagc aacaggtcct gctggtccgc ggggagcgca
      361 gggaccgaaa ggggagaaag gcgaccccgg tcccagaggt gagcgtggag aaaccgggcc
      421 gatggggccg cccgggacat cagacgggaa gagccgggtt gtgggtatca ggctgggtaa
      481 taaacagact tatgcgccgc aaacagacag cactacatgg gcagtggatt tggatattgg
      541 tgcaatgatt acggggattg gcggttacaa cgacggaaac aaaaccctta ttgacagggt
//
LOCUS       DAZGQL010000380          598 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.384, whole genome shotgun sequence.
ACCESSION   DAZGQL010000380 DAZGQL010000000
VERSION     DAZGQL010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..598
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.384"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..>598)
                     /locus_tag="ACHKIR_005434"
     rRNA            complement(<1..>598)
                     /locus_tag="ACHKIR_005434"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          119 a          194 c          131 g          154 t
ORIGIN      
        1 tccagatctc tacgcatttc accgctacac ctggaattct acccccctct acgagactca
       61 agcttgccag tatcagatgc agttcccagg ttgagcccgg ggatttcaca tctgacttaa
      121 caaaccgcct gcgtgcgctt tacgcccagt aattccgatt aacgcttgca ccctccgtat
      181 taccgcggct gctggcacgg agttagccgg tgcttcttct gcgggtaacg tcaatgagca
      241 aaggtattaa ctttactccc ttcctccccg ctgaaagtac tttacaaccc gaaggccttc
      301 ttcatacacg cggcatggct gcatcaggct tgcgcccatt gtgcaatatt ccccactgct
      361 gcctcccgta ggagtctgga ccgtgtctca gttccagtgt ggctggtcat cctctcagac
      421 cagctaggga tcgtcgccta ggtgagccgt taccccacct actagctaat cccatctggg
      481 cacatccgat ggcaagaggc ccgaaggtcc ccctctttgg tcttgcgacg ttatgcggta
      541 ttagctaccg tttccagtag ttatccccct ccatcaggca gtttcccaga cattactc
//
LOCUS       DAZGQL010000381          597 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.385, whole genome shotgun sequence.
ACCESSION   DAZGQL010000381 DAZGQL010000000
VERSION     DAZGQL010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..597
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.385"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..330)
                     /locus_tag="ACHKIR_005435"
     CDS             complement(<1..330)
                     /locus_tag="ACHKIR_005435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO9993003.1"
                     /translation="MRTDNQVHKALFTIPTAAYSAVPANIKPLPEQRRITGHKQTDAY
                     LWILEVIHLNEAVHLDAAEAALEKLKITPEEASERYGRYLQEINVDPFQIAFATIGMD
                     NPAQAIRN"
     gene            complement(330..>597)
                     /locus_tag="ACHKIR_005436"
     CDS             complement(330..>597)
                     /locus_tag="ACHKIR_005436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFO9993004.1"
                     /translation="TWATINSGFMKKFRVPAGVTHLIIFADRDEYSATGMAAACECAH
                     ANLQARNDIQRVSVRWPDHDDFNNMLMNGDQVRELVFYRKKAVA"
BASE COUNT          136 a          156 c          138 g          167 t
ORIGIN      
        1 attcctgatc gcctgcgccg ggttatccat gcctatggtt gcgaaagcaa tctggaaagg
       61 atcaacatta atctcctgca aataacgtcc ataccgttca ctcgcttctt ccggcgttat
      121 tttgagtttc tccagtgctg cttcggctgc gtccagatgt acggcctcgt tcaggtgaat
      181 aacctccaga atccagagat aagcatcagt ctgtttatgt ccggtgatcc tcctttgttc
      241 tggcagaggt ttgatatttg ccggaacggc gctgtacgct gccgtcggga tagtgaataa
      301 agctttatgt acctgattgt ccgtacgcat cacgcaaccg ccttttttct gtaaaaaacc
      361 agttcccgaa cctgatcgcc gttcatgagc atattgttga aatcatcatg atcaggccac
      421 cggacactta cccgctgaat gtcatttcgg gcctggagat ttgcatgtgc gcattcacag
      481 gccgcagcca ttccggtagc actgtattca tcgcgatcgg caaaaataat cagatgggtg
      541 actcccgccg gtacgcggaa ctttttcata aaaccactgt taatggttgc ccaggta
//
LOCUS       DAZGQL010000382          592 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.386, whole genome shotgun sequence.
ACCESSION   DAZGQL010000382 DAZGQL010000000
VERSION     DAZGQL010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..592
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.386"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..>592)
                     /locus_tag="ACHKIR_005437"
     CDS             complement(<1..>592)
                     /locus_tag="ACHKIR_005437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HFO9993005.1"
                     /translation="PAFLTNRQELISRGLLVPALFLAEFLILVPLYFIFFRKREGLGR
                     GSFRLDVFFTLFSLIMIIQLFVPYLLAVRKTEEWMISQMSFSGAILWINIFSSVLLVP
                     VYEEIVFRGCLFNSFKFWFNDNIYISAIVTSVIFSALHLQYTDFRTFLMLFLISLVLI
                     SAKIKSNGLLMPILLHMAMNAVITGIQYTLQYHITV"
BASE COUNT          232 a           98 c          105 g          157 t
ORIGIN      
        1 cagactgtta tgtgatattg taacgtatat tgtattcctg ttatgactgc attcatcgcc
       61 atgtgaagta atatgggcat taacaggcca ttcgacttta tttttgcact gataagaact
      121 aacgatatca gaaagagcat caaaaatgta cgaaagtcag tatattgtag atgtaatgca
      181 gaaaatatga ctgatgttac aatagcagag atgtaaatat tgtcattaaa ccagaattta
      241 aaagagttaa aaagacaccc tcgaaataca atctcttcat atacaggaac aagaagtacg
      301 gaagaaaata tattaatcca taaaatggcc ccggaaaaag acatttgcga gatcatccat
      361 tcttccgttt tcctcactgc cagcagataa gggacaaata actgaataat cattattaaa
      421 gagaaaagag taaaaaaaac atccagtcga aaagaacctc ttcctaatcc ttctcttttt
      481 cggaagaaaa taaaatacag tggaacaaga attagaaatt cagcaagaaa taatgctgga
      541 acaagaagtc ccctggatat aagttcctgc ctgtttgtaa ggaatgcagg aa
//
LOCUS       DAZGQL010000383          586 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.387, whole genome shotgun sequence.
ACCESSION   DAZGQL010000383 DAZGQL010000000
VERSION     DAZGQL010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..586
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.387"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>586
                     /locus_tag="ACHKIR_005438"
     CDS             <1..>586
                     /locus_tag="ACHKIR_005438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFO9993006.1"
                     /translation="GEANFIENIVWNKRIPKNDKGIGNIHEYILAYAKNNEISKEFLM
                     RKDGIDDVYQFIEKLKKEKVPLDKAEQQLKKFYSSNGYDRGITLYNGLNEDYRPWGKI
                     NMSWPNADSFGPTYEVLHPLTNNPVKIPDRGWRWKEGTFNHIAKRIDGKYADIKKLHD
                     GSVICGGIWFARTEKTQPSSVKFLDEVEEFLLRSI"
BASE COUNT          215 a           83 c          124 g          164 t
ORIGIN      
        1 tggcgaagca aatttcatag aaaatattgt atggaataaa cgtatcccta aaaatgataa
       61 gggtattggt aatatccatg agtacatcct tgcttatgcg aagaataacg aaataagtaa
      121 agaattttta atgcggaaag atggtattga tgatgtatat cagtttattg aaaagttaaa
      181 aaaggaaaag gtgccattag ataaagcaga gcagcaactc aaaaaattct acagctccaa
      241 cggatatgat cgtggaatta ctctttacaa cggtttgaac gaggattata ggccatgggg
      301 taaaattaat atgagttggc ctaatgcaga ttcatttgga cccacgtatg aagtattgca
      361 ccctttgact aataaccctg tcaaaatccc agacagaggc tggcgttgga aagaaggcac
      421 ttttaatcat attgcaaaaa gaatagatgg aaagtatgca gatatcaaaa aactacatga
      481 tggtagcgtt atttgtggtg gaatttggtt tgcccgtaca gaaaaaaccc aacccagttc
      541 ggttaaattt ttagatgaag ttgaagagtt tttactaaga tcaatt
//
LOCUS       DAZGQL010000384          580 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.388, whole genome shotgun sequence.
ACCESSION   DAZGQL010000384 DAZGQL010000000
VERSION     DAZGQL010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..580
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.388"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>580
                     /locus_tag="ACHKIR_005439"
     CDS             <1..>580
                     /locus_tag="ACHKIR_005439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFO9993007.1"
                     /translation="KQYGDFENGIPVHDTIARVVSCISPAKFHECFINWMRDCHSSDD
                     KDVIAIDGKTLRHSYDKSRRRGAIHVISAFSTMHSLVIGQIRTDEKSNEITAIHELLN
                     MLDIKGKIITTDAMGCQKDTAEKIQKQGGDYLFAVKGNQGRLNKAFEEKFPLKELNNP
                     EHDSYAISEKSHGREEIRLHIVCDVPDELIDFM"
BASE COUNT          189 a          108 c          130 g          153 t
ORIGIN      
        1 aagcaatatg gtgattttga aaatggtatt cctgttcacg ataccattgc cagagttgta
       61 tcctgtatca gtcctgcaaa atttcacgag tgctttatta actggatgcg tgactgccat
      121 tcttcagatg ataaagacgt tattgcaatt gatggaaaaa cgctccggca ttcttatgac
      181 aagagtcgcc gcaggggagc gattcatgtc attagtgcgt tctcaacaat gcacagtctg
      241 gtcatcggac agatcaggac ggatgagaaa tccaatgaga tcacagctat ccatgaactt
      301 cttaacatgc tggatattaa aggaaaaatc atcacaactg atgcgatggg ttgccagaaa
      361 gataccgcag agaagataca aaaacagggc ggtgattatt tattcgctgt aaaaggaaat
      421 caggggcggc ttaataaagc ctttgaggaa aaattcccac tgaaagaatt aaataatcca
      481 gagcatgaca gttacgcaat tagtgaaaaa agtcacggca gagaagaaat ccgtcttcac
      541 attgtttgcg atgtccctga tgaacttatt gatttcatgt
//
LOCUS       DAZGQL010000385          577 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.389, whole genome shotgun sequence.
ACCESSION   DAZGQL010000385 DAZGQL010000000
VERSION     DAZGQL010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.389"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            240..>577
                     /locus_tag="ACHKIR_005440"
     CDS             240..>577
                     /locus_tag="ACHKIR_005440"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HFO9993008.1"
                     /translation="MDKVLDSAILSSANKRKGILAIGAHPDDIELGCGASLARLAQKG
                     IYIATVVMTTGNSGVDGIIDRHEESRNALKILGCHQTIHLNFADTRAHLQLNDMISAL
                     ENIIKNQIPS"
BASE COUNT          187 a          124 c          103 g          163 t
ORIGIN      
        1 taaatcacat ttaaatacaa tatataaaac aaaataacca tacaaaccaa acacacaaac
       61 acttaacata tgaactacat aaagattata cagtcttatc cccgtacctg gcagcccctt
      121 gtttttcagc tatcagctat ttttaacact taaaaattag catctgtctg cgtcagaggt
      181 tacgtctctg cgggcgtcgt tgtgcttgta gtataataaa taaaatggag tgtcataaag
      241 tggataaggt tttagattca gcgatccttt cttcggcaaa taaaagaaag ggtatccttg
      301 ccattggcgc gcatcctgac gatatagaat taggctgtgg cgcatcgcta gctcgccttg
      361 cgcaaaaagg aatttatatt gctaccgtgg tgatgactac cggtaattct ggcgtagatg
      421 gaataatcga tcgccacgaa gaatcacgca acgctctaaa gatattaggg tgccaccaaa
      481 ctattcatct taattttgct gacacccgcg ctcatttaca gctcaatgat atgatttctg
      541 ccctggaaaa tatcataaaa aatcaaattc cctctga
//
LOCUS       DAZGQL010000386          572 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.390, whole genome shotgun sequence.
ACCESSION   DAZGQL010000386 DAZGQL010000000
VERSION     DAZGQL010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..572
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.390"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>572
                     /locus_tag="ACHKIR_005441"
     CDS             <1..>572
                     /locus_tag="ACHKIR_005441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004218023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primase-helicase zinc-binding domain-containing
                     protein"
                     /protein_id="HFO9993009.1"
                     /translation="EAAKGHWPEILKHYGLPPVTGKKHFKGECPVCGARGKFRIDDRD
                     GTGTWICVCGSGDGMKLIALTQKRAFHEICAEIDRITGNEYRRDKPPVICTSESLRSR
                     VQRRFSALTALQGTSGAEYLRSRGIFSLPVEGVRFNSQQNYNGRMFQSLYALATDDKG
                     ELCYLHQTLLDGAKKADIGISARRLKSLQD"
BASE COUNT          154 a          128 c          158 g          132 t
ORIGIN      
        1 gaagcagcga aaggccactg gcctgaaatt ttaaaacatt acggactgcc gccagtcacg
       61 ggtaaaaagc acttcaaagg ggaatgtccg gtttgtggtg ccagaggtaa gttccgtatt
      121 gatgaccgtg atggcaccgg aacgtggatc tgtgtttgtg gtagcggtga cggcatgaaa
      181 ctgatcgctc tgacacaaaa aagagcgttt catgaaattt gcgctgaaat agaccgcatc
      241 acggggaatg aataccggag agataaaccg cctgtgatct gtacgtcgga aagcctgaga
      301 tcgcgcgttc agcgtcgatt ttcagcgctc acggcgctac agggaacatc tggtgctgaa
      361 tatcttcgtt cccgtggcat attcagtctt ccggttgagg gagttcgctt caacagccag
      421 caaaattata acgggcgcat gttccagtct ctttatgccc tggcgaccga tgacaaggga
      481 gaactgtgtt acctgcatca gacattgctt gatggagcta aaaaagcaga cattggtatc
      541 agcgccagaa ggctcaaatc tttacaggac ga
//
LOCUS       DAZGQL010000387          562 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.391, whole genome shotgun sequence.
ACCESSION   DAZGQL010000387 DAZGQL010000000
VERSION     DAZGQL010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..562
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.391"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..99)
                     /locus_tag="ACHKIR_005442"
     CDS             complement(<1..99)
                     /locus_tag="ACHKIR_005442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFO9993010.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLT"
     gene            363..>562
                     /locus_tag="ACHKIR_005443"
     CDS             363..>562
                     /locus_tag="ACHKIR_005443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005767712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
                     /protein_id="HFO9993011.1"
                     /translation="MTSIQQRAELHRQIWQIANDVRGSVDGWDFKQYVLGALFYRFIS
                     ENFSSHMENGDDSICYAKLDDNV"
BASE COUNT          168 a          119 c          139 g          136 t
ORIGIN      
        1 ggtgagtttg cgcagatgtt ccaggatgtg acgggaaagt gagtaaggca gggaggagag
       61 cggtttttca attgcctcaa tggcatcaga cggattcata catgtattcc cggtgaaaaa
      121 agacaaaacc ccgcacaccg gaaaggaggc gggggactca caacgataat atctgtctga
      181 aattatttag aatgtacctg gtgcactgca aaggaaaggc agtattccgt tcctcctcct
      241 tgtcgtgaat accccaggta tctcacaatg tacaccgact tactccgggg ctgcctgaac
      301 cacaagattg ctgtagaatt gcccccaatg attcaggtcc agaccaggaa aatggattaa
      361 aaatgacaag tattcaacaa cgagcagaac tgcaccgcca gatatggcaa attgccaatg
      421 atgtcagagg ttctgttgat ggatgggatt ttaagcaata cgttctgggc gcacttttct
      481 accgttttat cagcgaaaat ttttccagcc atatggaaaa tggtgatgac agcatctgtt
      541 atgcaaaact ggatgacaac gt
//
LOCUS       DAZGQL010000388          556 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.392, whole genome shotgun sequence.
ACCESSION   DAZGQL010000388 DAZGQL010000000
VERSION     DAZGQL010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..556
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.392"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..256
                     /locus_tag="ACHKIR_005444"
     CDS             <1..256
                     /locus_tag="ACHKIR_005444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO9993012.1"
                     /translation="FAIAIRNHWHVENKLHWRLDVVMNEDDCKIRRGNAAELFSGIRH
                     IAINILTNDKVFKAGLRRKMRKAAMDRNYLASVLAGSGLS"
     gene            458..>556
                     /locus_tag="ACHKIR_005445"
     CDS             458..>556
                     /locus_tag="ACHKIR_005445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9993013.1"
                     /translation="MELKKLMEHISIIPDYRQAWKVEHKLSDILLLT"
BASE COUNT          185 a          104 c          118 g          149 t
ORIGIN      
        1 gttcgccata gcaatccgaa accactggca cgtggagaat aagctgcact ggcgtctgga
       61 cgtggtaatg aatgaagacg actgcaaaat aagaagagga aatgcagcag aattattttc
      121 agggatacgg cacattgcta ttaatatttt gacgaatgat aaggtattca aggcagggtt
      181 aagacgtaag atgcgaaaag cagccatgga cagaaactat ctcgcgtcag tccttgcggg
      241 gagcgggctt tcgtaatctt gccctgatca gaccaccgtt gatcaccata tccatgagtt
      301 tatgaataaa gggcagggaa agatcacgca acgctgcttt tttgtactaa ataattcgca
      361 ctaaatattc aataattgag atattcctta ttacctaaag ctgttttcca ttgcttatac
      421 atgatcaaat actccttaca taattaagga gaacaaaatg gaacttaaaa aattgatgga
      481 acatatttct attatccccg attacagaca agcctggaaa gtggagcata aattgtcaga
      541 tattctactg ttgact
//
LOCUS       DAZGQL010000389          539 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.393, whole genome shotgun sequence.
ACCESSION   DAZGQL010000389 DAZGQL010000000
VERSION     DAZGQL010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..539
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.393"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            170..532
                     /locus_tag="ACHKIR_005446"
     CDS             170..532
                     /locus_tag="ACHKIR_005446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000721276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9993014.1"
                     /translation="MKKYILVSLMGLSLNAFAGGSGWNPDRVDPSRCVMATGSGPTSA
                     GYMVYRTTDSVCMQGINEGKVKGVHIYDGHHEFNDGTVDTFSGNVTPISPLTLKTDVQ
                     KVNKVGIKRWWYKASWIK"
BASE COUNT          172 a           80 c          132 g          155 t
ORIGIN      
        1 tagatactag atatgctaat ggttttatac tctaaataat tcgagttaca ggtagtgtat
       61 aagtgagttc ccaagagctt gcataagtaa gtgactgagg gagcgtaagt gcagtacatg
      121 caacttgttg catgacaaat atattatcta ttaacaataa gaggttataa tgaagaaata
      181 tattttagta tctctgatgg gattaagttt gaacgcattt gcgggtggca gcggctggaa
      241 cccagacagg gtagacccga gccgttgtgt tatggcgacg gggtcaggtc ctacctctgc
      301 gggttatatg gtataccgaa cgactgactc agtgtgtatg cagggtataa atgaaggaaa
      361 agtgaaaggt gtccatattt atgatgggca tcatgagttc aatgatggta ctgttgacac
      421 gtttagcggt aatgttaccc caatatcacc tttgacatta aaaactgatg ttcaaaaagt
      481 taataaagtt ggcatcaaac gctggtggta taaagctagc tggattaaat aaaaaatac
//
LOCUS       DAZGQL010000390          529 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.394, whole genome shotgun sequence.
ACCESSION   DAZGQL010000390 DAZGQL010000000
VERSION     DAZGQL010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..529
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.394"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>529
                     /locus_tag="ACHKIR_005447"
     CDS             <1..>529
                     /locus_tag="ACHKIR_005447"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFO9993015.1"
                     /translation="NGASKSLDELKSIPDVIIKHLGQNRGIATAQNVGLRISLSLCSK
                     YALLLDQDSEIETDLVSKLLDGYITLEQKGIKLAAVGPRPFDLFENKKMKANIQRDIQ
                     IFEGFSLTKQIIASGKLINLEVLDQVGFMEDDLFIDGVDHEWCWRAGRLGYKIAIIEN
                     AIMKHRLGDARGNFLG"
BASE COUNT          183 a           73 c          108 g          165 t
ORIGIN      
        1 taatggtgca tcaaaaagtt tagacgaatt aaaaagtatt ccagatgtta taattaagca
       61 ccttggtcaa aaccgtggta ttgcaacggc acaaaatgtc gggctaagaa tttctctatc
      121 tttatgctca aagtatgcgc tattactgga tcaagattca gaaattgaaa cagatttagt
      181 gtcaaaactt ctcgacggtt acattacatt agaacaaaaa ggcatcaaac ttgccgctgt
      241 tggtccccga ccgtttgatt tattcgaaaa caaaaaaatg aaagcaaaca ttcaaagaga
      301 tattcaaata tttgagggtt tttctttgac gaaacagata atcgcttcag gtaagctaat
      361 taatttggaa gttttagatc aagtcggttt tatggaagat gatttattta ttgatggtgt
      421 tgatcatgaa tggtgttggc gtgctggaag attaggatat aaaattgcaa taatagaaaa
      481 tgctattatg aaacatcgat taggcgatgc acgtggtaat tttttagga
//
LOCUS       DAZGQL010000391          523 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.395, whole genome shotgun sequence.
ACCESSION   DAZGQL010000391 DAZGQL010000000
VERSION     DAZGQL010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..523
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.395"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>523
                     /locus_tag="ACHKIR_005448"
     CDS             <1..>523
                     /locus_tag="ACHKIR_005448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HFO9993016.1"
                     /translation="RKYKRFKAFSDFINKYIVCTPLNKDTKCFDIIFLGSDQIWNANY
                     TNGVDPNYYGQGPYCKAHKLVSYAASMGKLCLGKYEEQAFLSLINNIQQIGVRENYLK
                     TYIEEKTDLKCDVNLDPTLLLTKSDWDKLAAPNDTQERYLLIYEMHTHRSTNIIANKI
                     AKILNLKIKKLACR"
BASE COUNT          203 a           68 c           91 g          161 t
ORIGIN      
        1 aacgtaaata taaaaggttt aaagcattta gtgattttat aaataaatat attgtatgta
       61 cgccattgaa taaagatact aaatgtttcg atattatctt tcttggtagt gatcagatat
      121 ggaatgcaaa ttatactaac ggagtagacc ctaactatta tggacaagga ccttattgta
      181 aagcgcacaa attagtttct tatgcggcaa gtatgggtaa gctgtgtttg ggaaaatatg
      241 aggaacaagc attcttatct ttgataaata atatacagca gattggtgtt agagaaaatt
      301 atttaaagac gtacatagaa gaaaaaaccg atttaaaatg tgatgttaat cttgatccga
      361 ctcttctgct tactaaaagc gattgggata aactggcagc acctaacgat acacaagaac
      421 gctatcttct tatatatgaa atgcatactc acaggagtac aaatattatt gctaataaaa
      481 tagcaaaaat actaaattta aagattaaaa aattagcatg tcg
//
LOCUS       DAZGQL010000392          518 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.396, whole genome shotgun sequence.
ACCESSION   DAZGQL010000392 DAZGQL010000000
VERSION     DAZGQL010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..518
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.396"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>518
                     /locus_tag="ACHKIR_005449"
     CDS             <1..>518
                     /locus_tag="ACHKIR_005449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFO9993017.1"
                     /translation="ARIHLFSNAAHLSPGQVLEPQGDVIADLKEGIVLTLVTYRGARD
                     SRLHVSAWGMPYTERYCFRPAEIPRPVIPGTLPARIESRGKNDIYAHLDGQGRYRVKM
                     DFDREETEPGFGYLWLRMAKPCAGETLGWHTPLIDGTEVAIAYSNGDIDLPYIAYALH
                     DAEHPDLVSRDN"
BASE COUNT          105 a          148 c          165 g          100 t
ORIGIN      
        1 gcccgcattc acctgttcag caacgccgca cacctctccc ccgggcaggt gctggagccg
       61 cagggggatg tcattgcgga cctgaaggaa gggattgttc tcacactggt gacttaccgg
      121 ggggcccggg attcccggct gcatgtgtca gcatggggaa tgccttacac cgaacgttac
      181 tgtttccgtc cggcagaaat cccgcgtccg gtcatcccgg gtacgcttcc ggcacggatt
      241 gagagtcgcg ggaagaacga tatctatgcc catctggacg ggcaggggcg ttaccgggtg
      301 aagatggact ttgaccggga ggagacggaa cccggattcg gctacctgtg gctgcggatg
      361 gcaaaaccct gtgcaggtga aacgctgggc tggcatacgc cgctcattga tggcaccgaa
      421 gtggcgattg catacagcaa tggcgatatt gacctgccct acatcgcgta tgcactgcac
      481 gacgctgagc atcctgacct cgtgagccgg gacaatca
//
LOCUS       DAZGQL010000393          516 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.397, whole genome shotgun sequence.
ACCESSION   DAZGQL010000393 DAZGQL010000000
VERSION     DAZGQL010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..516
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.397"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(167..>516)
                     /locus_tag="ACHKIR_005450"
     CDS             complement(167..>516)
                     /locus_tag="ACHKIR_005450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000425544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9993018.1"
                     /translation="SCDYYNAGMDYQDNIALEQVVTWDSDNIFTPNIFEHNGRCFMKK
                     NETWMLLNGDATPNIRTMSNKKIECLEKSEVEWASRVCDSITDKERENLKALICSKPA
                     KSVNTGKRPALGY"
BASE COUNT          122 a          114 c           94 g          186 t
ORIGIN      
        1 aaaaaagcag cgttgcgtga tctttcccta gtccctgtcc cggattgtag taccggtgcc
       61 agttgtagtg cagttcggat tcgtcgtcat actgctagcc cggatggcgg agcagttgct
      121 ctagtcactc agaatattgt aatagtgata gagtgcagtg ccggtttcaa tatcctaacg
      181 ctggtctttt gcctgtatta actgatttag ctggtttgct acatattaat gccttcagat
      241 tttccctttc cttgtctgtg atactgtcgc aaaccctact agcccattct acttcactct
      301 tctccaggca ttctattttc ttattgctca ttgttctaat gtttggtgtt gcatctccat
      361 ttaataacat ccatgtttca tttttcttca taaaacatct tccattatgt tcaaagatat
      421 taggtgtgaa tatattatca gaatcccatg ttacgacttg ctcgagggct atattatctt
      481 gatagtccat gccagcatta taataatcac aagata
//
LOCUS       DAZGQL010000394          514 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.398, whole genome shotgun sequence.
ACCESSION   DAZGQL010000394 DAZGQL010000000
VERSION     DAZGQL010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.398"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..>514)
                     /locus_tag="ACHKIR_005451"
     CDS             complement(<1..>514)
                     /locus_tag="ACHKIR_005451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="contractile injection system protein, VgrG/Pvc8
                     family"
                     /protein_id="HFO9993019.1"
                     /translation="QNQSVPEVVEQVLRKHGLEGADFEFRLERAYPSREIITQWQETD
                     LQFIQRILSEVGIFWRTEMDDVRGLDTYIFADSQLNYQFDVCLPYSEPSGLFDGAAES
                     VWDVRTWHNVATGTVSTRDYNYRTASMPMDATVSVRNDAVTTGEHYRDAPAYREAGDD
                     TSPEPETESGA"
BASE COUNT          111 a          163 c          134 g          106 t
ORIGIN      
        1 gcaccagatt cggtttccgg ctccgggctg gtgtcatccc ccgcttcacg ataagccggt
       61 gcatcgcggt aatgctcccc ggtggtgacc gcatcattac gcacgctgac cgtcgcatcc
      121 atcggcatgg aggcagtccg gtagttgtaa tcacgcgtgg agaccgtgcc ggtggcgacg
      181 ttgtgccatg tccgtacatc ccagaccgat tctgcagcac cgtcaaaaag gcccgacggc
      241 tcgctgtacg gcagacacac atcaaactgg tagttaagct ggctgtcggc aaaaatgtac
      301 gtatccagtc cgcgcacatc atccatctcc gtgcgccaga aaatccccac ctcagacaga
      361 atgcgctgaa tgaactgcaa atccgtttcc tgccactggg tgataatttc acgcgacggg
      421 taagcgcgtt ccagtctgaa ttcaaaatca gcgccctcca gcccgtgttt gcgcagcacc
      481 tgctccacca cctcagggac ggactggttc tgaa
//
LOCUS       DAZGQL010000395          508 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.399, whole genome shotgun sequence.
ACCESSION   DAZGQL010000395 DAZGQL010000000
VERSION     DAZGQL010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.399"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>508
                     /locus_tag="ACHKIR_005452"
     CDS             <1..>508
                     /locus_tag="ACHKIR_005452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFO9993020.1"
                     /translation="KGGWNFYQYPLNPITNIDPLGLETLKCIKPLHSMGGTGERSGPD
                     IWGNPFYHQYLCVPDGKGDYTCGGQDQRGESKGDGLWGPGKASNDTKEAAGRCDLVET
                     DNSCVENCLKGKFKEVRPRYSVLPDIFTPINLGLFKNCQDWSNDSLETCKMKCSGNNI
                     GRFIRFVFT"
BASE COUNT          161 a           79 c          127 g          141 t
ORIGIN      
        1 aaggggggat ggaattttta tcagtatccg ttgaatccga tcactaatat agatccttta
       61 ggattagaaa cactaaaatg cattaagcca ctgcattcaa tgggcggaac tggtgaaaga
      121 agcggtccag atatatgggg gaatccgttc tatcatcaat atctttgtgt cccagatggt
      181 aaaggggact atacttgtgg tggccaagac caacggggag aatcaaaagg agatggtcta
      241 tgggggccag gtaaagcaag taatgataca aaagaagctg ctggccgttg tgacctcgtt
      301 gaaaccgata atagttgtgt ggagaactgt ttaaaaggga agtttaaaga ggtaaggccg
      361 cgttattctg tattgcctga tatattcaca cctataaatt tagggctatt taaaaactgc
      421 caagactggt ctaatgattc tttagaaaca tgtaagatga agtgctccgg aaataacatt
      481 ggacgtttta ttagatttgt atttaccg
//
LOCUS       DAZGQL010000396          506 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.400, whole genome shotgun sequence.
ACCESSION   DAZGQL010000396 DAZGQL010000000
VERSION     DAZGQL010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..506
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.400"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..483
                     /locus_tag="ACHKIR_005453"
     rRNA            <1..483
                     /locus_tag="ACHKIR_005453"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          127 a          123 c          147 g          109 t
ORIGIN      
        1 tgtcgtcagc tcgtgttgtg aaatgttggg ttaagtcccg caacgagcgc aacccttatc
       61 ctttgttgcc agcggtccgg ccgggaactc aaaggagact gccagtgata aactggagga
      121 aggtggggat gacgtcaagt catcatggcc cttacgacca gggctacaca cgtgctacaa
      181 tggcgcatac aaagagaagc gacctcgcga gagcaagcgg acctcataaa gtgcgtcgta
      241 gtccggattg gagtctgcaa ctcgactcca tgaagtcgga atcgctagta atcgtggatc
      301 agaatgccac ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac accatgggag
      361 tgggttgcaa aagaagtagg tagcttaacc ttcgggaggg cgcttaccac tttgtgattc
      421 atgactgggg tgaagtcgta acaaggtaac cgtaggggaa cctgcggttg gatcacctcc
      481 ttaccttaaa gaagcgtact ttgcag
//
LOCUS       DAZGQL010000397          505 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.401, whole genome shotgun sequence.
ACCESSION   DAZGQL010000397 DAZGQL010000000
VERSION     DAZGQL010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..505
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.401"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>505
                     /locus_tag="ACHKIR_005454"
     CDS             <1..>505
                     /locus_tag="ACHKIR_005454"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFO9993021.1"
                     /translation="VARMDTDDICVPERFEKQISYFQKNPSTIILGGAIDEYDETFKE
                     RRGRRFSCCSHSDIVSYSLFRNPFNHMTVMFNKEFILGIGGYKHHHYMEDYNLWLRCI
                     ANGAICSNIDNTLVLARTGDAMIERRRGRDYLKSEIELARLKIKCFPNKKAKIILITF
                     SRLIVRIL"
BASE COUNT          174 a           64 c          108 g          159 t
ORIGIN      
        1 agttgctagg atggatactg atgatatctg tgttcctgaa aggtttgaga aacaaataag
       61 ttattttcag aaaaatccaa gtacgattat attaggtggg gccattgatg aatatgatga
      121 gacttttaaa gaaaggagag ggcgtagatt ttcatgttgc tcgcacagtg atatagtgag
      181 ctattctctc tttagaaatc cattcaacca tatgactgta atgttcaata aagaatttat
      241 cttgggaata ggtggttaca agcatcatca ttacatggaa gattataatt tatggctaag
      301 gtgcattgct aatggtgcca tatgttctaa tattgataat acgctggtcc ttgcacgaac
      361 aggtgatgca atgatagaac gaaggagggg aagggattat ttaaaaagtg aaatcgaatt
      421 ggcacgcttg aaaattaagt gttttccaaa taaaaaagca aaaatcattt taataacttt
      481 ttctagactt atagtacgta tacta
//
LOCUS       DAZGQL010000398          504 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.402, whole genome shotgun sequence.
ACCESSION   DAZGQL010000398 DAZGQL010000000
VERSION     DAZGQL010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..504
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.402"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..78
                     /locus_tag="ACHKIR_005455"
     CDS             <1..78
                     /locus_tag="ACHKIR_005455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9993022.1"
                     /translation="LRRKMRKAAMDRNYLASVLAGSGLS"
     gene            88..504
                     /locus_tag="ACHKIR_005456"
                     /pseudo
     CDS             88..504
                     /locus_tag="ACHKIR_005456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238907.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          148 a           96 c          127 g          133 t
ORIGIN      
        1 ttaagacgta agatgcgaaa agcagccatg gacagaaact acctggcgtc agtccttgct
       61 gggagcgggc tttcgtaatc tttccctggg cggtatatca tccaggactc gattgggctg
      121 aaggggggat ggaatctgta ccaatatccg ctgaatccag tgcaacacgt tgatccatta
      181 gggcttagta ctatgataat aggtaacggc cccgttcctg ataatccttt tgggcatgct
      241 gcggcggcaa atcgatatgg attaatgagt tcgggaactg gggatgagat gggagcctct
      301 gtctctgatt attttaaaaa gatgcaaccc cgcagagata cctggatttg gataatagat
      361 acaacagatg aagaagaaca atgtatgatg aataaggcaa tagaccttaa taaaaaacta
      421 aaaactatta acctcgggcc tcttcctgtt ttaaataatt gttttagccg aacgaacatg
      481 atttttgagg cgtgcgggtt taac
//
LOCUS       DAZGQL010000399          489 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.403, whole genome shotgun sequence.
ACCESSION   DAZGQL010000399 DAZGQL010000000
VERSION     DAZGQL010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..489
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.403"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>489
                     /locus_tag="ACHKIR_005457"
     CDS             <1..>489
                     /locus_tag="ACHKIR_005457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="9-O-acetyl-N-acetylneuraminic acid deacetylase"
                     /protein_id="HFO9993023.1"
                     /translation="STGGSASAVPWVCGDTTYFWKQRHAEGYASVYGGYKGKESQNIF
                     FVPLMTDENGANVQTNDPAEDPDLEAVGYYGSKWRNDQKTWTSVDRASHFSSWARRGI
                     ISDRLATAILVHAGRTAEFITGKQPDTVKPTGPSGEGPEREPEAPVSNRTLMSLLASG
                     EDL"
BASE COUNT          120 a          117 c          157 g           95 t
ORIGIN      
        1 agcacaggag gcagcgcatc agcggtaccg tgggtttgcg gggacacaac gtatttctgg
       61 aagcagcgtc atgcggaagg gtatgcatca gtatacggag ggtataaggg taaggagtcg
      121 cagaacattt tctttgtacc gttaatgacg gatgagaatg gtgcgaatgt gcagaccaat
      181 gacccggcag aagacccgga cctggaagcc gtcggttatt acggttcaaa gtggcgtaat
      241 gaccagaaga cctggacatc tgtggacagg gccagccatt tcagttcatg ggctcgccgt
      301 gggattattt ccgaccgtct ggcaacggcg attctggtgc atgccgggag aaccgctgaa
      361 ttcattaccg gaaaacagcc tgatacggtg aagcccaccg gaccttccgg tgaaggcccg
      421 gagagagagc cggaagcccc ggtcagtaac cgaaccctga tgagtctgct ggcgtccggt
      481 gaagacctg
//
LOCUS       DAZGQL010000400          478 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.404, whole genome shotgun sequence.
ACCESSION   DAZGQL010000400 DAZGQL010000000
VERSION     DAZGQL010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..478
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.404"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            137..>478
                     /locus_tag="ACHKIR_005458"
     CDS             137..>478
                     /locus_tag="ACHKIR_005458"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFO9993024.1"
                     /translation="MKSGGKWEEYTIERLFSIQTPKKKFNANKLTFNGNYPYVARGSN
                     NNGIKGYIDEDELYLNPGNTISFGQDTATMFYQENAYFTGDKIKIFSLRHGELNSRIA
                     VFLISAMKKSFS"
BASE COUNT          166 a           78 c          102 g          132 t
ORIGIN      
        1 ccagaccgac actggaggat tttaaaaaga cggttggcga ctatcttgcg tgggaggtgt
       61 caaaacttct tcaggggcgt gatgcgtcag aaaaaaagca gtaaactccc gtcttgcaga
      121 gcttgagcgg gagtttatta aatctggtgg taaatgggaa gaatacacaa tcgaaagatt
      181 gttcagtatt cagacaccta aaaagaaatt taatgccaat aaattaacat ttaatggcaa
      241 ctatccttat gttgccagag gaagtaacaa taacggcata aaaggatata tagatgaaga
      301 tgaattatat ctcaacccgg ggaatacaat tagctttggt caggatactg cgaccatgtt
      361 ctatcaggaa aacgcatatt tcacaggtga taaaataaag attttttctc tcagacatgg
      421 agagttaaat tcgcgaattg ctgtttttct tatatcagca atgaaaaaat ctttttca
//
LOCUS       DAZGQL010000401          473 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.405, whole genome shotgun sequence.
ACCESSION   DAZGQL010000401 DAZGQL010000000
VERSION     DAZGQL010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.405"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(165..>473)
                     /gene="hcp"
                     /locus_tag="ACHKIR_005459"
     CDS             complement(165..>473)
                     /gene="hcp"
                     /locus_tag="ACHKIR_005459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system effector Hcp"
                     /protein_id="HFO9993025.1"
                     /translation="TKEIDSSSPYLYKAVTTGQTLKSAEFKWYRINDAGQEVEYFNTK
                     LENVKVVKVNPVMHDIKNPAYEKHNHLEQVELRYEKITWTYKDGNIIHSDAWNERTTA
                     "
BASE COUNT          112 a          128 c          108 g          125 t
ORIGIN      
        1 ttcactcact gtcagaagac cttcagacac gctaccgccc ttcgccataa cccgggcgca
       61 tcaggtctgt gttcaggatt gagaattctt tcttcagcac ccgccaacag atactgaaga
      121 aaaaagaaaa atgacggaca ggataccctg cccgtccggg agaattacgc agtggtacgt
      181 tcattccacg cgtcagaatg aatgatgtta ccgtccttat aagtccaggt gattttctcg
      241 tagcgcagct ccacctgctc aaggtggtta tgcttctcgt aggcgggatt cttgatgtcg
      301 tgcatgaccg ggttaacctt caccaccttc acgttctcaa gcttcgtatt gaaatactcc
      361 acttcctgac cggcatcatt gattctgtac cacttaaatt cagcagactt cagggtctgc
      421 cccgtggtca ccgccttgta aaggtagggg ctggaggaat caatttcctt ggt
//
LOCUS       DAZGQL010000402          472 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.406, whole genome shotgun sequence.
ACCESSION   DAZGQL010000402 DAZGQL010000000
VERSION     DAZGQL010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.406"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            180..440
                     /gene="iraP"
                     /locus_tag="ACHKIR_005460"
     CDS             180..440
                     /gene="iraP"
                     /locus_tag="ACHKIR_005460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HFO9993026.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRNYVKKLLKHPRQ"
BASE COUNT          163 a           80 c          101 g          128 t
ORIGIN      
        1 attaaaagcc tatattttgt gtgggtaatt attaaaataa gagaaacgtt tcgctggtaa
       61 tcaaacaaaa aatatttgcg caaagtattt cctttgtcat aaaaataata cttccagaca
      121 ctatgaagtt gtgaaacata atgttaactt ctccatactt tggataagga aatacagaca
      181 tgaaaaatct cattgctgag ttgttattta agcttgccca aaaagaagaa gagtcgaaag
      241 aactgtgcgc gcaggtagaa gctttggaga ttatcgtcac tgcaatgctt cgcaatatgg
      301 cgcaaaatga ccaacagagg ttgattgatc aggtagaggg ggcgctgtac gaggtaaagc
      361 ccgatgccag cattcctgac gacgatacgg agctgctgcg caattatgta aagaagttat
      421 tgaagcatcc tcgtcagtaa aaagttaatc ttttcaacag ctgtcataaa gc
//
LOCUS       DAZGQL010000403          446 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.407, whole genome shotgun sequence.
ACCESSION   DAZGQL010000403 DAZGQL010000000
VERSION     DAZGQL010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..446
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.407"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>446
                     /locus_tag="ACHKIR_005461"
     CDS             <1..>446
                     /locus_tag="ACHKIR_005461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO9993027.1"
                     /translation="GGQTPYDDKPLSVTGIDALGLSINKITTNNMEFNGDVAIVSEDS
                     PYLHNGIVFLNGIPFKIIGVIPKMKMDFLDSLGLSKYQSNNQLFVPLSTLFRFKLSSN
                     IDNVKIVLSDKVGENDINTTKKTLGEEKIPEYNIITALDVRTTVDK"
BASE COUNT          163 a           73 c           84 g          126 t
ORIGIN      
        1 tggcggtcaa acaccttacg atgacaaacc tttatcagtg acaggtattg atgcgctagg
       61 tttatccata aataaaataa ccaccaataa catggaattt aatggagatg ttgctattgt
      121 tagtgaggat agtccatatt tacacaatgg gattgttttc cttaatggga taccgtttaa
      181 aattattggc gtaattccaa aaatgaaaat ggacttcctg gacagtcttg ggttatcgaa
      241 atatcagagc aacaatcagc tctttgtccc cttaagtaca ttattcagat tcaagctaag
      301 cagtaatatt gacaatgtta agatagtgtt aagcgataaa gttggcgaaa atgatattaa
      361 cacaacaaaa aaaacgcttg gtgaggaaaa aataccagaa tacaatatta taacagctct
      421 tgatgtaaga accaccgtcg acaaag
//
LOCUS       DAZGQL010000404          442 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.408, whole genome shotgun sequence.
ACCESSION   DAZGQL010000404 DAZGQL010000000
VERSION     DAZGQL010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.408"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..189
                     /locus_tag="ACHKIR_005462"
     CDS             <1..189
                     /locus_tag="ACHKIR_005462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9993028.1"
                     /translation="CGKARLVLTKDGQIFLNGTKIHLQGKEQVNGDSLLINWNCAASK
                     SPPKTPDEKQDTPDMREY"
     gene            256..>442
                     /locus_tag="ACHKIR_005463"
     CDS             256..>442
                     /locus_tag="ACHKIR_005463"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9993029.1"
                     /translation="MSGKPAARQGDMTQYGGPIVQGSAGVRIGAPTGVACSVCPGGMT
                     SGNPVNPLLGAKVLPGET"
BASE COUNT          119 a           98 c          137 g           88 t
ORIGIN      
        1 tgtggtaagg caaggctggt gctgactaag gacggacaaa tttttctcaa cggcacaaaa
       61 attcatttgc agggtaagga gcaggttaat ggtgactcac tgttgattaa ctggaactgt
      121 gcagcctcga aatccccacc gaagacccct gatgaaaagc aggatacgcc ggatatgaga
      181 gagtactgat cccgtacctg ttcggtattc agtaacaaac aagcattaaa aataaacaga
      241 agaaggaatt gaatcatgag cggaaaacca gcggcgcgtc agggagatat gactcagtat
      301 ggcggtccca ttgtccaggg ttcggcaggt gtaagaattg gcgcgcccac cggcgtggcg
      361 tgctcggtgt gtccgggcgg gatgacttcg ggcaacccgg taaatccgct gctgggggcg
      421 aaggtgctgc ccggcgagac gg
//
LOCUS       DAZGQL010000405          441 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.409, whole genome shotgun sequence.
ACCESSION   DAZGQL010000405 DAZGQL010000000
VERSION     DAZGQL010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..441
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.409"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..>441)
                     /locus_tag="ACHKIR_005464"
     CDS             complement(<1..>441)
                     /locus_tag="ACHKIR_005464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFO9993030.1"
                     /translation="SGRALAPLGQIASLATRFQQARLAMQGVNDIVRRPVERESQTHY
                     LTVKDIQGEFRFSDVSFSYKPGLPDALCGLSLCIRPGERVGIIGSIGCGKSTLLKMMV
                     GLYRQNRGQITLDGVAMQQLDPCFVRDKVFLLEQNPRLFLGTLR"
BASE COUNT          115 a          134 c          105 g           87 t
ORIGIN      
        1 tcacgcagag ttcccagaaa aagccgggga ttctgttcca gaagaaacac tttatcccgg
       61 acaaaacagg ggtccagttg ctgcatcgca accccgtcaa gagtaatttg tcccctgttc
      121 tgacggtaca gccccaccat cattttcagc agcgtcgatt taccacaacc tattgagcca
      181 ataataccca cccgctctcc gggccggata cagagcgaca gaccgcacaa tgcgtcgggt
      241 aatccgggct tataagaaaa ggagacatca ctgaaacgaa actctccctg gatatccttc
      301 actgtcagat aatgtgtctg actttcccgc tccaccggac ggcggacaat gtcattcacc
      361 ccctgcatgg ccaggcgggc ctgctgaaaa cgggtggcca gtgatgctat ctgaccaagc
      421 ggtgccagcg cacgaccgga a
//
LOCUS       DAZGQL010000406          430 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.410, whole genome shotgun sequence.
ACCESSION   DAZGQL010000406 DAZGQL010000000
VERSION     DAZGQL010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.410"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..>430)
                     /locus_tag="ACHKIR_005465"
     CDS             complement(<1..>430)
                     /locus_tag="ACHKIR_005465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ImpA family type VI secretion system protein"
                     /protein_id="HFO9993031.1"
                     /translation="SQEERLSLISRANEALSLWDNWLHPFTSGSETGDDPAYDDNFQL
                     MREEINKISGTDSALLCELAQKCLCECARDIRVVTWYVLARLTRDGERGLSEGLLLLV
                     AMLTRYGQACHPRRPAARKAALEWLNSAKVTDALLLWPGV"
BASE COUNT          101 a          128 c          109 g           92 t
ORIGIN      
        1 tccaccccgg gccataacag cagtgcatca gtcacttttg cactgttcag ccactccagc
       61 gcagctttcc gggcggcagg acgccgggga tgacatgcct gtccgtagcg ggtcagcatc
      121 gcaacaagaa gtaataagcc ttcggacaac cctctttcac catcccgggt gagacgggcc
      181 agcacgtacc aggtcacgac acgaatatct ctggcgcatt cgcaaaggca cttctgagcc
      241 agttcgcaca acagcgctga atcagtacct gaaatcttat tgatctcttc acgcatcagc
      301 tgaaaattat catcgtatgc aggatcgtcc ccggtttcag atccggaggt aaatggatgc
      361 agccagttat cccagagcga cagggcctca tttgcgcggg aaatgaggct cagcctttct
      421 tcctggctgg
//
LOCUS       DAZGQL010000407          427 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.411, whole genome shotgun sequence.
ACCESSION   DAZGQL010000407 DAZGQL010000000
VERSION     DAZGQL010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..427
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.411"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..349
                     /locus_tag="ACHKIR_005466"
     CDS             <1..349
                     /locus_tag="ACHKIR_005466"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9993032.1"
                     /translation="SFWFIFVSRNKNSKRPDDAWVQPNTGAYSSTFGVPENMQSVLSN
                     ADELLLIHLTKEKRSESLVCLNIQQNPQGVSRSVPGVYESHNIDRDDIYEWLFYPMVV
                     LCVPVNGGLPLDR"
BASE COUNT          115 a           92 c          101 g          119 t
ORIGIN      
        1 ctcattttgg tttattttcg tatcgcgaaa caaaaattcg aagcggccag atgacgcatg
       61 ggtgcaacca aataccgggg cgtattcatc aacctttggt gttcctgaaa acatgcagtc
      121 cgtgctctcg aatgcagatg aattgctgtt gatccactta acaaaagaaa aaaggtctga
      181 atcgcttgtc tgtctgaata ttcaacaaaa tcctcaaggt gtgtctcgat cagttccggg
      241 tgtttacgaa agtcataata ttgaccggga cgatatttat gagtggttgt tttatcccat
      301 ggttgtgctc tgcgtccctg taaacggagg tctgccgctg gatagataag cataagtaat
      361 ctcctggtgg aatgtaatcc acgatcatcc ggggaaatta agaacccgcc agtgcccacc
      421 actggtg
//
LOCUS       DAZGQL010000408          418 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.412, whole genome shotgun sequence.
ACCESSION   DAZGQL010000408 DAZGQL010000000
VERSION     DAZGQL010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..418
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.412"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            135..>418
                     /locus_tag="ACHKIR_005467"
     CDS             135..>418
                     /locus_tag="ACHKIR_005467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HFO9993033.1"
                     /translation="MSSSFQNEIPKARVNLKLDLHTGGGQKKVELPLKLLVAGDFSNG
                     QEERPLSERIKMDVNKNNFNAVLADISPSVRLNVENTLAGDGTEESISLT"
BASE COUNT          117 a           81 c          117 g          103 t
ORIGIN      
        1 aaacgatcga tatgtctgtc atattgagaa tttgcagatc ttcgtcctat aatgggtgaa
       61 attaaatcag tgcacaaggg gaggccttct gtggcgatgg gctccctggg atgcaggttt
      121 cacaggagaa cgtcatgagc agttcgtttc agaatgagat cccgaaggca agggtgaatc
      181 tgaaattaga cctacataca ggtggtgggc agaagaaagt ggagcttccg ctcaaactgc
      241 tggttgccgg tgatttcagt aacggacagg aagagcgccc gctgtcagag cgcataaaaa
      301 tggatgttaa taaaaataac tttaatgctg ttctggctga tatctcgccg tcagtacgcc
      361 tgaatgtgga gaacacactt gccggtgatg gtactgaaga gagcatcagc ctgacttt
//
LOCUS       DAZGQL010000409          413 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.413, whole genome shotgun sequence.
ACCESSION   DAZGQL010000409 DAZGQL010000000
VERSION     DAZGQL010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..413
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.413"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..200)
                     /locus_tag="ACHKIR_005468"
     CDS             complement(<1..200)
                     /locus_tag="ACHKIR_005468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009309580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFO9993034.1"
                     /translation="MDDLTLRYYEAEMRYLREAGKEFARAHPDRAAMLNLDKPGACDP
                     GVERLFEGFAFLMGRLREKLDD"
     gene            complement(234..>413)
                     /locus_tag="ACHKIR_005469"
     CDS             complement(234..>413)
                     /locus_tag="ACHKIR_005469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004173386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HFO9993035.1"
                     /translation="TLEDWEPALLFEVRARRLKLLRMKAGRSESDKARLMPEMDTLLA
                     GLIAIDPARAMVLCG"
BASE COUNT          101 a          130 c          101 g           81 t
ORIGIN      
        1 tcatcatcca gcttttcacg caaacgtccc atcaggaagg caaagccttc aaacagacgt
       61 tccacaccgg gatcgcaggc gccaggctta tccagattca gcattgctgc ccgatccgga
      121 tgagcacggg caaactcttt accggcttcc cgcagatagc gcatctctgc ttcgtaatag
      181 cgcagggtta aatcatccat gcacggcatt cctgaaaatg attaaagaga acatcagcca
      241 cagagcacca tggcacgggc cgggtcaatg gcgatcagcc ccgccagcag cgtatccatc
      301 tcaggcatca gacgggcttt atcagattcg ctgcgaccgg ctttcatccg tagcagcttc
      361 aggcgtcggg cccggacttc aaacagcaaa gcgggctccc agtcttccag cgt
//
LOCUS       DAZGQL010000410          405 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.414, whole genome shotgun sequence.
ACCESSION   DAZGQL010000410 DAZGQL010000000
VERSION     DAZGQL010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..405
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.414"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..>405)
                     /locus_tag="ACHKIR_005470"
     CDS             complement(<1..>405)
                     /locus_tag="ACHKIR_005470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004160280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFO9993036.1"
                     /translation="DAVLSLLAPFASTVQGGQRTRRLILLIWLCVLTALGISALNNRD
                     LIRQVSTDLQRWNAVPKGHYRPEAESLAALKQDALLLEQWQRQGVPLRYSLGYYPGQR
                     LWLALQQAIDTRVPPPSPEPEPVPEIVRLDSLA"
BASE COUNT           93 a          123 c          115 g           74 t
ORIGIN      
        1 gccaggctat cgagtcgtac aatttccggc accggttctg gttcagggga tggtggtgga
       61 acccgggtat cgatagcctg ctgcagggcc agccacagac gttgtcccgg gtaatagccc
      121 aggctgtaac gtaaaggcac tccctgacgc tgccactgct caagcagaag cgcatcctgt
      181 ttcagtgcgg ccagcgattc cgcctcagga cggtagtgcc ccttcgggac agcattccag
      241 cgctgtaaat cggtgcttac ctggcggatc agatcacgat tgttaagcgc agaaatcccc
      301 agcgcagtca ggacacacag ccatatcagc aaaatcagac gacgggtacg ctggcctccc
      361 tgtaccgtcg acgcaaaagg tgccagcagc gacagaaccg catcc
//
LOCUS       DAZGQL010000411          404 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.415, whole genome shotgun sequence.
ACCESSION   DAZGQL010000411 DAZGQL010000000
VERSION     DAZGQL010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.415"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>404
                     /locus_tag="ACHKIR_005471"
     CDS             <1..>404
                     /locus_tag="ACHKIR_005471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFO9993037.1"
                     /translation="FGVTYTGNYEFPQPLHDALDKFQADTGIDIDMHIDAASGGFLAP
                     FVAPDIVWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQI
                     GTFAINFSRPAGQVIAQYYEFLRLGREGYTKV"
BASE COUNT           76 a          121 c          115 g           92 t
ORIGIN      
        1 ttcggcgtga cctacaccgg taactatgag ttcccgcagc cgctgcacga tgcgctggat
       61 aaattccagg ccgacaccgg tatcgacatc gacatgcaca tcgacgccgc cagcggtggc
      121 ttcctggcac cgttcgtcgc cccggatatc gtctgggact tccgcctgcc gcgtgtgaaa
      181 tcgatcagtg cttcaggcca taaattcggt ctggctccgc tgggctgcgg ctgggttatc
      241 tggcgtgatg aagaagcgct gccgcaggaa ctggtgttca acgttgacta cctcggcggt
      301 cagattggta ctttcgccat caacttctcc cgcccggcgg gtcaggtgat tgcacagtac
      361 tatgaattcc tgcgcctcgg tcgtgaaggc tataccaaag taca
//
LOCUS       DAZGQL010000412          403 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.416, whole genome shotgun sequence.
ACCESSION   DAZGQL010000412 DAZGQL010000000
VERSION     DAZGQL010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..403
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.416"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>403
                     /locus_tag="ACHKIR_005472"
     CDS             <1..>403
                     /locus_tag="ACHKIR_005472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFO9993038.1"
                     /translation="KRVVKKELADGSVTHSGYDAAGRLTAQTDAAGRRTEYGLNVVSG
                     DITDITTPDGRETKFYYNDGNQLTAVVYPDGLESSRAYDELDRLVSETSRSGETVRYR
                     YDDVHSELPATTTDATGSTRQMTWSRYGQLLA"
BASE COUNT           98 a          101 c          151 g           53 t
ORIGIN      
        1 tgaagcgggt ggtgaaaaaa gaactggcgg acggcagcgt cacgcacagc gggtatgacg
       61 cggcaggaag gctcacggca cagacggacg cggcgggacg gcggacagag tacggcctga
      121 atgtggtgtc cggcgatatc acggatatca ccacgccgga cgggcgggag acgaaatttt
      181 actataacga cgggaaccag ttaacggcgg tggtgtaccc ggacgggctg gagagcagcc
      241 gggcatatga tgagctggac agactggtat cggagacatc gcgcagcggg gagacagtac
      301 gctaccgcta cgatgacgtg cacagtgagt taccggcgac gacaacggat gcgacgggca
      361 gcacccggca gatgacctgg agccgctacg ggcagttgct ggc
//
LOCUS       DAZGQL010000413          396 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.417, whole genome shotgun sequence.
ACCESSION   DAZGQL010000413 DAZGQL010000000
VERSION     DAZGQL010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..396
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.417"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(166..>396)
                     /gene="tuf"
                     /locus_tag="ACHKIR_005473"
     CDS             complement(166..>396)
                     /gene="tuf"
                     /locus_tag="ACHKIR_005473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFO9993039.1"
                     /translation="RHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVT
                     LIHPIAMDDGLRFAIREGGRTVGAGVVAKVLG"
BASE COUNT          124 a           87 c           85 g          100 t
ORIGIN      
        1 aagcatttta aaaacagaaa cattcatatt taagatatta aatttaattt atattttaaa
       61 tatgaataat ttattcgttc tgacagtacg aataagatat gcagtcaaca aatgcaaaaa
      121 gggcgccgaa gcgccctttt taattcgaaa ctaattaacg tgtaattagc ccagaacttt
      181 agcaacaacg cccgcgccaa cggtacggcc gccttcacgg attgcgaaac gcagaccgtc
      241 gtccatcgcg atcgggtgga tcagggtaac aaccattttg atgttgtcgc ccggcataac
      301 catctctacg ccttccggca gttcgatggt accagtcacg tcagtagtac ggaagtagaa
      361 ctgcggacgg tagcctttga agaacggagt atgacg
//
LOCUS       DAZGQL010000414          388 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.418, whole genome shotgun sequence.
ACCESSION   DAZGQL010000414 DAZGQL010000000
VERSION     DAZGQL010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..388
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.418"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..>388)
                     /locus_tag="ACHKIR_005474"
     CDS             complement(<1..>388)
                     /locus_tag="ACHKIR_005474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RTX toxin"
                     /protein_id="HFO9993040.1"
                     /translation="ANVGIPADKVTQDGEYTVKAEITDPAGNTSGQGKATQFGVDTVA
                     PSEPALKAENDGSVSATLPDGANKGDKVEVTFTDEEGNEQKVTLEKGDGNWSSDKPEL
                     IPDSTDNKVTVPADKVKNNTEVTATAK"
BASE COUNT           64 a          121 c           80 g          123 t
ORIGIN      
        1 ctttcgctgt tgccgtcacc tctgtgttgt tcttcacttt gtctgccgga actgtcactt
       61 tattatcggt gctgtccggg atcagttcag gcttgtcgga actccagttg ccatcccctt
      121 tctccagcgt gactttctgc tcattaccct cttcatccgt aaaggtcact tccaccttgt
      181 cacctttgtt cgcaccatct ggcagagtcg ctgatacaga accatcattc tcagccttca
      241 gtgctggttc tgacggagct acagtatcca cgccaaactg ggtagcttta ccctgaccac
      301 tggtgttgcc tgccgggtct gtgatttcag ccttaactgt gtattcccca tcctgcgtca
      361 ctttatccgc aggaatgccc acattcgc
//
LOCUS       DAZGQL010000415          383 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.419, whole genome shotgun sequence.
ACCESSION   DAZGQL010000415 DAZGQL010000000
VERSION     DAZGQL010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..383
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.419"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..294)
                     /locus_tag="ACHKIR_005475"
     CDS             complement(<1..294)
                     /locus_tag="ACHKIR_005475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3289 family protein"
                     /protein_id="HFO9993041.1"
                     /translation="MNNILGSSINMPCTLFETITLFDDFSADDMQYGDMEEQDFLSLG
                     LSDISAKVDPYRLIKYHFPGPNSTYGAFSVPTSGTKISQSECIDILFAEMKDLA"
BASE COUNT          138 a           60 c           65 g          120 t
ORIGIN      
        1 tgccagatct ttcatttctg caaataaaat atcaatacat tcactttgtg atatttttgt
       61 tccagaagtt ggaacactaa aagcaccata agtagaattc ggcccaggaa aatggtattt
      121 aatgaggcgg taaggatcaa cttttgcaga gatgtcgctc aagccgagtg agagaaaatc
      181 ttgttcttcc atatccccat attgcatatc atcagcagaa aaatcatcga ataaagtaat
      241 agtttcgaaa agtgtacagg gcatattaat tgatgagcct aatatgttgt tcatttatat
      301 tctcctttct tataattaag ggcaatgtta atttgataag aaataatagg ctaaaaaaat
      361 tttaaaaaag aaatgcactt cac
//
LOCUS       DAZGQL010000416          376 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.420, whole genome shotgun sequence.
ACCESSION   DAZGQL010000416 DAZGQL010000000
VERSION     DAZGQL010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.420"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>376
                     /locus_tag="ACHKIR_005476"
     CDS             <1..>376
                     /locus_tag="ACHKIR_005476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFO9993042.1"
                     /translation="GWTPRGELAAVYDRSNTQVRSFTYDDKYRGRMVAHRHTGRPEIR
                     YRYDSDGRVTEQLNPAGLSYTYQYEKDHITITDSLDRREVLHTQGEGGLKRVVKKEHA
                     DGSVTQSQFDAVGRLKAQTDAAG"
BASE COUNT           93 a          103 c          129 g           51 t
ORIGIN      
        1 ggctggacgc cgcgcggcga actggcggcg gtgtatgacc gcagcaatac acaggtgcgc
       61 agctttactt acgatgataa ataccggggc cggatggtgg cgcaccgtca cacgggccgg
      121 ccggaaatcc gttaccgtta cgacagtgac gggcgggtga cagaacagct aaacccggca
      181 ggcttaagct acacgtatca gtatgagaaa gaccacatca ccatcaccga cagcctggac
      241 cgccgtgaag tgctgcacac gcagggcgaa ggcgggctga agcgggtggt gaaaaaggaa
      301 cacgcggacg gcagcgtcac gcagagtcag tttgacgcgg tgggcaggct taaggcacag
      361 acggatgccg caggcc
//
LOCUS       DAZGQL010000417          372 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.421, whole genome shotgun sequence.
ACCESSION   DAZGQL010000417 DAZGQL010000000
VERSION     DAZGQL010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.421"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>372
                     /locus_tag="ACHKIR_005477"
     CDS             <1..>372
                     /locus_tag="ACHKIR_005477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007370995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HFO9993043.1"
                     /translation="HHPEFQKVESLWRGVKSLVDKTDFRRNVKIELLDLSKDDLRQDF
                     EDAPEIIQSGLYLQTYVAEYDTPGGEPIAALISAWEFDASAQDVALLKNISRVAASAH
                     MPFIGSVGPAFFRKDTMEEVAA"
BASE COUNT           77 a           80 c          116 g           99 t
ORIGIN      
        1 tcatcatccg gaatttcaga aagtggagtc cctgtggcgg ggcgtgaagt ctctggtgga
       61 taaaacggac ttccgccgta atgtgaaaat tgaactgctg gatctgtcca aagacgatct
      121 gcgtcaggat tttgaggatg cgccggaaat tattcagagt ggtctttatc tgcagactta
      181 tgtggcggag tacgatacgc cgggtggtga acccattgca gccctgattt ctgcctggga
      241 gtttgatgcg tctgcgcagg atgtggcgct gctgaaaaat atttcccggg tggctgcgtc
      301 cgcgcatatg ccatttattg gttcagttgg cccggctttc ttccggaagg atacgatgga
      361 agaggtggcc gc
//
LOCUS       DAZGQL010000418          372 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.422, whole genome shotgun sequence.
ACCESSION   DAZGQL010000418 DAZGQL010000000
VERSION     DAZGQL010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.422"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>372
                     /locus_tag="ACHKIR_005478"
     CDS             <1..>372
                     /locus_tag="ACHKIR_005478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFO9993044.1"
                     /translation="NETAKQFNTSIIVYLIDPKYFADLPTSQFWSYATYFRVLSFEYL
                     SESISTLLYLDADVVCKGSLKPLTEIIFKDEFAAVIPDNDSTQAACAKRLNIPEMNGR
                     YFNAGVIYVNLKKWHEANLTP"
BASE COUNT          114 a           71 c           71 g          116 t
ORIGIN      
        1 ttaatgaaac ggcaaaacaa tttaatacct caattattgt atatttaatt gaccccaaat
       61 actttgctga tctgccgacg tcacagtttt ggtcgtacgc aacatacttc agggtattgt
      121 cttttgaata tctgagtgaa agtatttcta cactgctgta tctggatgcc gatgttgttt
      181 gtaaaggaag cctgaaacct ctcacagaaa ttatatttaa agatgagttt gctgcggtca
      241 ttcctgacaa tgatagtact caggcggcat gtgcaaaacg cctcaacatt cccgaaatga
      301 atggacgtta tttcaatgca ggcgttatct atgtcaatct taaaaaatgg catgaagcaa
      361 atttgacacc gt
//
LOCUS       DAZGQL010000419          366 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.423, whole genome shotgun sequence.
ACCESSION   DAZGQL010000419 DAZGQL010000000
VERSION     DAZGQL010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.423"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(41..154)
                     /locus_tag="ACHKIR_005479"
                     /pseudo
     CDS             complement(41..154)
                     /locus_tag="ACHKIR_005479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518820.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          119 a           57 c           58 g          132 t
ORIGIN      
        1 aagatatcag aatcttatga ttcgggtgaa attctaacta aatcaacatg tcactaaaat
       61 catcatagcc attagctatt tcggctaaaa tagagattac atgtcttcgg cccatcttac
      121 ttaaggagtg tagttccgtt gtaagttttt ccatagcttg catttgctaa gtttcgaaca
      181 aggaattttc tgttggtaat ctctaaaaag atggcatggt ttacaatgat ttttgttacc
      241 ttttgattat tatgaacaac tgtccatgat ttcgtttaag aatgaagaga aatcactaaa
      301 cgaactgaat atattttcgg tgccaatatt atctctaatt tcaaaaaagt tacttttaat
      361 gtcata
//
LOCUS       DAZGQL010000420          363 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.424, whole genome shotgun sequence.
ACCESSION   DAZGQL010000420 DAZGQL010000000
VERSION     DAZGQL010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..363
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.424"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(55..>363)
                     /gene="ybfB"
                     /locus_tag="ACHKIR_005480"
     CDS             complement(55..>363)
                     /gene="ybfB"
                     /locus_tag="ACHKIR_005480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbfB"
                     /protein_id="HFO9993045.1"
                     /translation="LFRAIWLALSLLILFFSMHRLSLLDSTRDVSELISLMSYGMMVI
                     CFPTGIVFFIALIFIGTVSDIIGVRIDSKYIMAIIIWLYFLSGGYIQWFVLSKRIINK
                     "
BASE COUNT          143 a           68 c           50 g          102 t
ORIGIN      
        1 atacgggata tcaatgccca ggttacatat aataacaaga gcaattaact taatttattt
       61 gtttataatg cgcttactta atacaaacca ttgaatatac cctcctgaca gaaaataaag
      121 ccatattatt atcgccataa tatacttact atcaatcctt acgccaataa tgtctgaaac
      181 agtccctata aatattagag caataaagaa tactatgcct gttggaaaac atattaccat
      241 cattccataa gacatgagac taattaactc gctcacatca cgagtggagt ctaatagtga
      301 aagtctatgc atggaaaaga atagtattaa aagcgataaa gccagccata tagctctgaa
      361 taa
//
LOCUS       DAZGQL010000421          354 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.425, whole genome shotgun sequence.
ACCESSION   DAZGQL010000421 DAZGQL010000000
VERSION     DAZGQL010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..354
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.425"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>354
                     /locus_tag="ACHKIR_005481"
     CDS             <1..>354
                     /locus_tag="ACHKIR_005481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9993046.1"
                     /translation="PVTVTSKTDGVADAPALTIPEVADSVANAAELKDGLQAEVKLPA
                     GTVEGAVITLTVTHPDKTTRTETHTVSKDEAADGTVSMVVPKGSVVDGQNSVSVSLTQ
                     GSNPAKAGNKVEFVV"
BASE COUNT           94 a           71 c          112 g           77 t
ORIGIN      
        1 tccggtcact gtgacgtcaa aaactgatgg tgtggcagat gctccggccc tgaccattcc
       61 agaagtggct gatagtgtgg ccaatgcggc tgagctgaaa gatggtttac aggcagaagt
      121 caagttgccg gcaggaacag tggaaggtgc tgtcattaca ctgacagtga ctcatccgga
      181 taaaaccacc agaaccgaga cgcatactgt tagcaaagat gaggctgcag acggtactgt
      241 gtcaatggtt gttccgaagg gaagtgttgt tgacggtcag aacagtgtga gtgtcagcct
      301 gactcagggc agtaacccgg ctaaagcggg gaataaagtg gagtttgtgg ttga
//
LOCUS       DAZGQL010000422          341 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.426, whole genome shotgun sequence.
ACCESSION   DAZGQL010000422 DAZGQL010000000
VERSION     DAZGQL010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.426"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(104..>341)
                     /locus_tag="ACHKIR_005482"
     CDS             complement(104..>341)
                     /locus_tag="ACHKIR_005482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000425776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HFO9993047.1"
                     /translation="TDMTLDMLKQVDWVQVLTSVAKTVGVILVAVGVGGLVVTLGIPE
                     AIIGAFLLIVRLAISAWGTLATILGTGTAVLAVS"
BASE COUNT           84 a          107 c           71 g           79 t
ORIGIN      
        1 gattgactgc cggactgccg tctttgtatt tcacggaggc ttccggcagc catttctcga
       61 tgtagttcag ctcaaaatca tgcaattcca tacctgctcc tgtttaacta actgccagta
      121 ccgccgtacc cgtaccgaga atcgtcgcca gtgttcccca ggcgcttatt gccagacgaa
      181 caatcagtaa aaacgcgcca ataatggctt ccgggatacc cagcgttacg accagtccgc
      241 cgacacccac agcaaccaga ataacgccga ccgttttcgc gacactggtt aaaacctgta
      301 cccagtccac ctgtttcagc atatcaagcg tcatatcggt g
//
LOCUS       DAZGQL010000423          339 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.427, whole genome shotgun sequence.
ACCESSION   DAZGQL010000423 DAZGQL010000000
VERSION     DAZGQL010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..339
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.427"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            24..100
                     /locus_tag="ACHKIR_005483"
     tRNA            24..100
                     /locus_tag="ACHKIR_005483"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:58..60,aa:Ile,seq:gat)
     gene            143..218
                     /locus_tag="ACHKIR_005484"
     tRNA            143..218
                     /locus_tag="ACHKIR_005484"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:176..178,aa:Ala,seq:tgc)
BASE COUNT           98 a           71 c           83 g           87 t
ORIGIN      
        1 aaaatgagca gtaaaacctc tacaggcttg tagctcaggt ggttagagcg cacccctgat
       61 aagggtgagg tcggtggttc aagtccactc aggcctacca aatttgcacg gcaaatttga
      121 agaggtttta actacatgtt atggggctat agctcagctg ggagagcgcc tgctttgcac
      181 gcaggaggtc tgcggttcga tcccgcatag ctccaccatc tctgtagtga ttaaataaaa
      241 aatacttcag agtgtacctg caaaggttca ctgcgaagtt ttgctcttta aaaatctgga
      301 tcaagctgaa aattgaaaca ctgaacaacg aaagttgtt
//
LOCUS       DAZGQL010000424          336 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.428, whole genome shotgun sequence.
ACCESSION   DAZGQL010000424 DAZGQL010000000
VERSION     DAZGQL010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..336
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.428"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>336
                     /locus_tag="ACHKIR_005485"
     CDS             <1..>336
                     /locus_tag="ACHKIR_005485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9993048.1"
                     /translation="KQAGNTQYDYDAAGRMVSRTRHRDGYRPETERFRWDSRDQLTGY
                     CSAQGEQWEYRYDASGRRTEKRCDRKKIRFTYLWDGDNIAEIREYRDDKLYSVRHLVF
                     NGFELISQQ"
BASE COUNT           88 a           89 c          109 g           50 t
ORIGIN      
        1 gaaacaggcc ggaaacacgc agtatgacta tgacgccgca ggccggatgg tcagccgcac
       61 cagacaccgt gacggctacc gcccggaaac agagcggttc cggtgggaca gccgtgacca
      121 gctgaccggg tattgcagcg cacagggtga gcagtgggaa taccgctacg acgccagcgg
      181 caggcggacg gaaaaacgct gcgaccggaa gaaaatccgt tttacgtacc tgtgggacgg
      241 cgacaatatt gcggaaatcc gggaataccg cgatgataaa ctgtacagcg tacggcacct
      301 ggtgtttaac ggctttgagc tgataagcca gcagtg
//
LOCUS       DAZGQL010000425          333 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.429, whole genome shotgun sequence.
ACCESSION   DAZGQL010000425 DAZGQL010000000
VERSION     DAZGQL010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.429"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..289
                     /gene="iraM"
                     /locus_tag="ACHKIR_005486"
     CDS             <1..289
                     /gene="iraM"
                     /locus_tag="ACHKIR_005486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HFO9993049.1"
                     /translation="TCGISFSAIWGDMKMIIWYQSTIFLPPGSIFTPVKSGIILKDKE
                     YPITIYNIAPFNKDLWSLLKSSQECPPGESEIPNKCLHNSCIIKICPYGLK"
BASE COUNT          113 a           59 c           54 g          107 t
ORIGIN      
        1 tacatgcggc atatcttttt cagccatatg gggtgatatg aaaatgatca tctggtatca
       61 atctactata tttctccctc ctggaagtat atttacaccg gttaagtctg gtattatcct
      121 taaggataaa gaatacccta ttactattta taacatcgca ccatttaaca aggatttatg
      181 gagtttactc aaaagcagtc aagagtgtcc tcctggagaa agcgaaatac caaataaatg
      241 tttgcataat agttgtatta taaaaatatg cccatatgga ctcaagtaat gggcttcata
      301 taaatatgct cgacatgcta ttaataatat cca
//
LOCUS       DAZGQL010000426          332 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.430, whole genome shotgun sequence.
ACCESSION   DAZGQL010000426 DAZGQL010000000
VERSION     DAZGQL010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.430"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..>332)
                     /gene="tssK"
                     /locus_tag="ACHKIR_005487"
     CDS             complement(<1..>332)
                     /gene="tssK"
                     /locus_tag="ACHKIR_005487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004171008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HFO9993050.1"
                     /translation="WSEGVLLSPQQFQQQAEWESFRSAGISALASPFPWGVEQVEFND
                     SLLSSGLIQITQLRLWLEDGTLIDAQRSDLPPAPRELDAGQLADRDAVTVVIALPHMQ
                     PGVANVEQ"
BASE COUNT           83 a           99 c           88 g           62 t
ORIGIN      
        1 tcctgctcaa cgttggcaac gcctggctgc atgtggggaa gtgcaataac gacagttacc
       61 gcatcgcggt cagccagctg accggcatct aactcacgtg gcgccggagg cagatcgctc
      121 ctttgagcat caataagtgt tccgtcttcc agccagaggc gtaactgcgt aatctggatc
      181 agcccggacg acagaagact gtcattaaac tcaacctgtt ccactcccca gggaaacggg
      241 gatgccagcg cagagatacc ggcactgcga aaagactccc attcagcctg ctgctgaaac
      301 tgctgagggg agagcaaaac accttcgctc ca
//
LOCUS       DAZGQL010000427          331 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.431, whole genome shotgun sequence.
ACCESSION   DAZGQL010000427 DAZGQL010000000
VERSION     DAZGQL010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..331
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.431"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>331
                     /locus_tag="ACHKIR_005488"
     CDS             <1..>331
                     /locus_tag="ACHKIR_005488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImcF-related family protein"
                     /protein_id="HFO9993051.1"
                     /translation="VRTRRDEIDWVLTDKTHQTGSDISPEALKSRLTERYFTDFGNAW
                     LNMLNSIQWHEPTSLSGAIAQLSLLGDVRQSPLVALMNTLNYQGKTGQKGEALADSVV
                     DSAKKLLD"
BASE COUNT           86 a           84 c           93 g           68 t
ORIGIN      
        1 gtcagaacac gccgcgatga aattgactgg gtactgacgg ataaaacaca tcagacaggg
       61 agcgatatct ctccggaggc cctgaagagc cgcctgactg aacgctattt taccgatttt
      121 ggtaatgcct ggctgaacat gctcaacagc atacagtggc atgaacccac gtcactttcc
      181 ggagccattg cccagttgag tctgctcggt gacgtccgcc agtctccgtt agttgcactg
      241 atgaacacgc tgaactacca ggggaaaacg gggcagaagg gggaggcact ggctgattca
      301 gtcgtggatt cagcgaaaaa gctgctcgat a
//
LOCUS       DAZGQL010000428          329 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.432, whole genome shotgun sequence.
ACCESSION   DAZGQL010000428 DAZGQL010000000
VERSION     DAZGQL010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..329
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.432"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>329
                     /locus_tag="ACHKIR_005489"
     CDS             <1..>329
                     /locus_tag="ACHKIR_005489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9993052.1"
                     /translation="VFNVVGENGQVTEAVLTQDGPVLENFTAGQSGTLAEGINNGIIW
                     GGIAFGAGLAAIALAAGGGGGSSRHKRATEGEQKPVQPEGGQEAGKTEVSSTGSTEAG
                     SGSSSGQV"
BASE COUNT           77 a           64 c          117 g           71 t
ORIGIN      
        1 gtgtttaatg tggtgggtga aaatggccag gttactgaag ctgtcctgac ccaggatggt
       61 cctgtactgg agaatttcac tgcagggcaa agtggcacac tggctgaagg tatcaataac
      121 gggattatct ggggtgggat tgcttttggt gcagggctgg cggctattgc actggctgcc
      181 ggaggcggtg gtggaagcag tcgtcataag cgtgcaactg aaggtgagca gaagccggtt
      241 cagcctgaag gcggtcagga ggctggaaaa acagaagtat catccacagg ctcaactgag
      301 gcgggaagtg gaagctcttc cggccaggt
//
LOCUS       DAZGQL010000429          328 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.433, whole genome shotgun sequence.
ACCESSION   DAZGQL010000429 DAZGQL010000000
VERSION     DAZGQL010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..328
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.433"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
BASE COUNT          137 a           53 c           64 g           74 t
ORIGIN      
        1 aatgccaatt accagatctt tattctgata catatcgcaa aatcagcatt tatcaaaact
       61 tatatccagg ggtaatacaa aaatgcagaa aattattccg gttagtgctt ttgatacaga
      121 tggttatacc gaatgattac cagcactgac attcacgagg agtgatgatt cggcagcatt
      181 taagggcata tcagaaaaag agaaggacat gaaagaggag agcaataaaa gacacggaaa
      241 ataaagaaga gagagaaaat atttcttaaa aacagaagat taacaaaaca catcaggagg
      301 cagagactcc tgattcagca atatcaca
//
LOCUS       DAZGQL010000430          328 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.434, whole genome shotgun sequence.
ACCESSION   DAZGQL010000430 DAZGQL010000000
VERSION     DAZGQL010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..328
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.434"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
BASE COUNT           97 a           86 c           50 g           95 t
ORIGIN      
        1 gtcatcccgc ccggacacac cgagcacgcc acgccggtgg gcgcgccaat tcttacacct
       61 gccgaaccct ggacaatggg accgccatac tgagtcatat ctccctgacg cgccgctggt
      121 tttccgctca tataaagtgc ccttttagtt attgttcata tccctgtgtt agcaacaaaa
      181 aaataaaatg acacacaaga ccagctaatg tatttatata tttttcacat aaacttattt
      241 gttctcaatc aacaacaact gttattacag gtcactttca atattttatt aaactaacat
      301 ttttagcaca gatgaattca ttatcaac
//
LOCUS       DAZGQL010000431          327 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.435, whole genome shotgun sequence.
ACCESSION   DAZGQL010000431 DAZGQL010000000
VERSION     DAZGQL010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..327
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.435"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>327
                     /locus_tag="ACHKIR_005490"
     CDS             <1..>327
                     /locus_tag="ACHKIR_005490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9993053.1"
                     /translation="FSYLALPENYNRLFLPNSKNKTNNGLKTLNSEAVGRLLAAGGVY
                     NGNIEGFRDTAEKLGGDAIKGYDQILNEKTAGIAIATASILLTKRSNVDTYTEINSYL
                     GKLRGQQ"
BASE COUNT          123 a           55 c           65 g           84 t
ORIGIN      
        1 ttcagctatt tggcactccc tgaaaattat aaccgcttgt ttttaccaaa ttcaaaaaac
       61 aaaacaaata atggtttaaa aacgttaaac agcgaagctg ttggcaggct acttgctgct
      121 ggtggcgtat acaatgggaa cattgaaggt ttcagagata ctgcggaaaa actgggtgga
      181 gatgcaatca aaggctatga tcaaatacta aatgaaaaaa cagcgggcat agcgatagca
      241 acagcatcta ttcttttaac aaagcgttct aatgttgata catatacaga aataaatagt
      301 tacttaggca aacttagagg tcaacaa
//
LOCUS       DAZGQL010000432          314 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.436, whole genome shotgun sequence.
ACCESSION   DAZGQL010000432 DAZGQL010000000
VERSION     DAZGQL010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..314
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.436"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            12..>314
                     /locus_tag="ACHKIR_005491"
     CDS             12..>314
                     /locus_tag="ACHKIR_005491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="very short patch repair endonuclease"
                     /protein_id="HFO9993054.1"
                     /translation="MPDMRLFKSKNTKLELRIRKPLFARGFRYRLHNSGLPGRPDMYF
                     PKYNAVIFIHGCFWHGHDNGCPISHTPKNNESYWQNKINKNQERDVKNQYVLASKNI"
BASE COUNT          111 a           54 c           56 g           93 t
ORIGIN      
        1 aaggaactta tatgcctgat atgaggttat ttaaatcaaa aaacaccaaa cttgagttaa
       61 gaatcaggaa gccgttgttt gccagagggt ttcgttatcg attgcataat agtggtttgc
      121 ctggtagacc agatatgtat ttccctaaat acaatgcagt catttttatc catggctgtt
      181 tttggcatgg gcatgataac ggctgcccta tatctcacac tcctaaaaat aatgaatcgt
      241 actggcaaaa taaaatcaat aaaaatcaag aaagagatgt caaaaatcaa tatgttcttg
      301 catctaaaaa catc
//
LOCUS       DAZGQL010000433          312 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.437, whole genome shotgun sequence.
ACCESSION   DAZGQL010000433 DAZGQL010000000
VERSION     DAZGQL010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..312
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.437"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>312
                     /locus_tag="ACHKIR_005492"
     CDS             <1..>312
                     /locus_tag="ACHKIR_005492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004160261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFO9993055.1"
                     /translation="LGKTTGEACSRLLMTLETADPDEAEGWLPGGVLHTDLLVLLRVY
                     LGYRSDVRLRLTVPVRLLPEPRLGKGRPVQLGRTGLLGLKAGKLSNGRESLTVSLGCY
                     EG"
BASE COUNT           56 a           85 c          108 g           63 t
ORIGIN      
        1 ctgggtaaaa cgacagggga agcatgcagc cggctcctga tgacactgga aacagcagat
       61 ccggacgagg cggaaggctg gttgcccgga ggtgttctcc ataccgattt gctggtcctg
      121 ctgcgcgttt atctcggcta ccgcagtgac gtccggctgc gccttactgt ccccgtgcgg
      181 ttgttgcctg agccccgcct gggcaaaggc aggcctgttc agctgggccg taccggttta
      241 ctggggctga aagcgggcaa actcagcaat ggccgggaga gtctgacggt cagtctgggt
      301 tgctacgaag gg
//
LOCUS       DAZGQL010000434          310 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.438, whole genome shotgun sequence.
ACCESSION   DAZGQL010000434 DAZGQL010000000
VERSION     DAZGQL010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..310
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.438"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..264)
                     /locus_tag="ACHKIR_005493"
     CDS             complement(<1..264)
                     /locus_tag="ACHKIR_005493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFO9993056.1"
                     /translation="MFPDFFMHIGQALDLVSRYDSLRNPLTSLGDYLDPELISRCLAE
                     SGTVTLRKRRLPLEMMVWCIVGMALERKEPLHQIVNRLDIMLPG"
BASE COUNT           89 a           75 c           85 g           61 t
ORIGIN      
        1 gcccggcagc atgatgtcca ggcgattcac aatctggtga agaggttctt tacgctcaag
       61 cgccatgcca acaatacacc agaccatcat ttcgagggga agacggcgct tgcgtagcgt
      121 tacagtacct gattcggcaa ggcaacgaga gatgagttcg gggtcgaggt aatcccccag
      181 agaagtcagt gggttacgca gagaatcgta acgggatacc agatcaagag cctgtccaat
      241 gtgcataaaa aaatccggaa acaagtgagc gtttccggat tcttacacag ccactggatc
      301 ggtcaactga
//
LOCUS       DAZGQL010000435          309 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.439, whole genome shotgun sequence.
ACCESSION   DAZGQL010000435 DAZGQL010000000
VERSION     DAZGQL010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.439"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>309
                     /locus_tag="ACHKIR_005494"
     CDS             <1..>309
                     /locus_tag="ACHKIR_005494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 1"
                     /protein_id="HFO9993057.1"
                     /translation="ESSNAIDNADVYHYHRPHLESRLKENSVVTIHHDLYDTDPWLDY
                     DKFHPRYMEAKKILCLNSSQQNYLNNKGLMNTEIIPHGYNSDIFCSVGAQKKIKNKVC
                     I"
BASE COUNT          117 a           54 c           50 g           88 t
ORIGIN      
        1 gaaagtagca acgctattga taatgctgat gtctatcact atcatcggcc tcatttagaa
       61 agcagactta aggaaaactc agtagtaaca atacatcatg acttgtatga tacagaccca
      121 tggttggatt atgataagtt tcatccccgt tatatggaag caaaaaaaat actgtgcctt
      181 aactcctctc agcaaaatta tttaaacaat aaaggcttaa tgaatactga aattatacca
      241 catggttaca attctgacat cttttgctca gtaggggcgc aaaaaaaaat aaaaaataaa
      301 gtttgcatt
//
LOCUS       DAZGQL010000436          308 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.440, whole genome shotgun sequence.
ACCESSION   DAZGQL010000436 DAZGQL010000000
VERSION     DAZGQL010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.440"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>308
                     /locus_tag="ACHKIR_005495"
     CDS             <1..>308
                     /locus_tag="ACHKIR_005495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004173387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ImcF-related family protein"
                     /protein_id="HFO9993058.1"
                     /translation="VNRTQIYQAQETAGQAADTTKPLAERLRSQLVLQQAIARLQHRE
                     ATGAPWYTRFGLNQDSDTLAALWPLYAKNNAQLMRDATADSLRQQLNAFVQLPPASDA
                     "
BASE COUNT           75 a           94 c           84 g           55 t
ORIGIN      
        1 tcgttaaccg tactcagatt tatcaggcgc aggaaacggc gggacaggca gcggatacaa
       61 caaaaccact ggctgaacgc ctgcgcagcc agcttgtact ccagcaggcc attgcccgtc
      121 tgcaacatcg tgaggcaacc ggtgcgccct ggtacacacg atttggtctt aatcaggaca
      181 gcgacacgct ggccgcgctc tggccgctgt atgcgaaaaa taacgcacag ctgatgaggg
      241 atgccacagc agacagcctc aggcagcagc tgaatgcctt tgtgcaactc cctcctgcca
      301 gtgatgcc
//
LOCUS       DAZGQL010000437          303 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.441, whole genome shotgun sequence.
ACCESSION   DAZGQL010000437 DAZGQL010000000
VERSION     DAZGQL010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..303
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.441"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>303
                     /locus_tag="ACHKIR_005496"
     CDS             <1..>303
                     /locus_tag="ACHKIR_005496"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9993059.1"
                     /translation="KQAAQSAVDKLQAGNDKSGLQGRLDGLTPAAVPAVTDKDGNGKA
                     DTADKALADAEAAVRAAEAKKDAADDAAAKADKDGNKLITPAEAKAVTDANAELATA"
BASE COUNT           79 a           71 c          119 g           34 t
ORIGIN      
        1 aaacaggcgg cacagtcagc ggtggataaa ctgcaggcgg gtaacgacaa gagcggtctg
       61 cagggacgtc ttgatggtct gaccccggcg gcggttccgg cggtgacgga taaggacggc
      121 aacggtaagg cggacacggc agacaaggct ctggcggatg cggaagccgc agtccgggcg
      181 gcggaagcga agaaagatgc agcagatgat gctgcagcga aagcagacaa ggacggtaac
      241 aagctgatta ccccggcgga agcgaaggcg gtgacggatg cgaatgcgga gctggcgaca
      301 gcg
//
LOCUS       DAZGQL010000438          300 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.442, whole genome shotgun sequence.
ACCESSION   DAZGQL010000438 DAZGQL010000000
VERSION     DAZGQL010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..300
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.442"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
BASE COUNT           85 a           60 c           57 g           98 t
ORIGIN      
        1 caggttgtaa aaatccccga caataaagct gtaaattaat tactgttctt aaataatgtt
       61 attcgttttc agtccggcac agatgctttt ctgctttgtc tgctgtacag gataatgagc
      121 ggaaaagaca attcatcatg tttagcgcat ctggtgaacc aatatcaccg ctgtcaataa
      181 tcacctgtaa caaagacagg ctttctgtta tctcgcagtg gacattatcg agtacttcaa
      241 cgcaattatt catttttcat tatctccgtt attccggggg aggagctatg ctgttaatga
//
LOCUS       DAZGQL010000439          296 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.443, whole genome shotgun sequence.
ACCESSION   DAZGQL010000439 DAZGQL010000000
VERSION     DAZGQL010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..296
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.443"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            4..>296
                     /locus_tag="ACHKIR_005497"
     CDS             4..>296
                     /locus_tag="ACHKIR_005497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ESPR domain-containing protein"
                     /protein_id="HFO9993060.1"
                     /translation="MKRHLNTCYRLVWNHITGAFVVASELARARGKRGGVAVALSLAA
                     VTSLPVLAADIVVHPGETVNGGTLVNHDNQFVSGTADGVTVSTGLELGPDSDE"
BASE COUNT           62 a           76 c           94 g           64 t
ORIGIN      
        1 ctgatgaaac gacatctgaa tacctgctac aggctggtat ggaatcacat tacgggcgct
       61 ttcgtggttg cctccgaact ggcccgcgca cggggtaaac gtggcggtgt ggcggttgca
      121 ctgtctcttg ccgcggtcac gtcactcccg gtgctggctg ctgacatcgt tgtgcacccg
      181 ggtgaaacag tgaatggcgg aacactggta aaccatgaca accagtttgt atccggaaca
      241 gctgatggcg tgactgtcag taccgggctt gagctggggc cggacagtga cgaaaa
//
LOCUS       DAZGQL010000440          291 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.denovo.444, whole genome shotgun sequence.
ACCESSION   DAZGQL010000440 DAZGQL010000000
VERSION     DAZGQL010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..291
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.denovo.444"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..193)
                     /locus_tag="ACHKIR_005498"
     CDS             complement(<1..193)
                     /locus_tag="ACHKIR_005498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000906748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5507 domain-containing protein"
                     /protein_id="HFO9993061.1"
                     /translation="MLVSKSNGHNVNTVLGSGSCNENNSSNPMELLAYSIINLICKEA
                     ASGMYRGALETLQKIMSECI"
BASE COUNT           92 a           57 c           39 g          103 t
ORIGIN      
        1 atatacattc agacattatt ttttgtaaag tttcaagagc accgcgatac atccctgagg
       61 cagcttcctt acaaattaaa tttataatac tataagctag tagctccatg gggttggaag
      121 aattgttttc attacaactt ccactaccca aaactgtatt aacgttatga ccattacttt
      181 tactaactaa cattgtctat tcctcagtta atgtctacat ggctatgttt aacgttatta
      241 ctgtttgtca ttataaaaaa ccgctcattt gagacaattg ctgacattaa c
//
LOCUS       DAZGQL010000441         7360 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.guided.163, whole genome shotgun sequence.
ACCESSION   DAZGQL010000441 DAZGQL010000000
VERSION     DAZGQL010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7360
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.guided.163"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(151..1128)
                     /gene="epmA"
                     /locus_tag="ACHKIR_005499"
     CDS             complement(151..1128)
                     /gene="epmA"
                     /locus_tag="ACHKIR_005499"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFO9993062.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1453..3261
                     /gene="frdA"
                     /locus_tag="ACHKIR_005500"
     CDS             1453..3261
                     /gene="frdA"
                     /locus_tag="ACHKIR_005500"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFO9993063.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAVIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            3254..3988
                     /gene="frdB"
                     /locus_tag="ACHKIR_005501"
     CDS             3254..3988
                     /gene="frdB"
                     /locus_tag="ACHKIR_005501"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFO9993064.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            3999..4394
                     /gene="frdC"
                     /locus_tag="ACHKIR_005502"
     CDS             3999..4394
                     /gene="frdC"
                     /locus_tag="ACHKIR_005502"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFO9993065.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4405..4764
                     /gene="frdD"
                     /locus_tag="ACHKIR_005503"
     CDS             4405..4764
                     /gene="frdD"
                     /locus_tag="ACHKIR_005503"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFO9993066.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            4827..5960
                     /locus_tag="ACHKIR_005504"
     CDS             4827..5960
                     /locus_tag="ACHKIR_005504"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFO9993067.1"
                     /translation="MFKMTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGI
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDDVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANASIGLFGALAVKPSGLSFEQAMQTRVFQPLKLTHTWINVPSAEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLKPLDITEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDIIINGSDNKIALAARPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            6049..6582
                     /gene="blc"
                     /locus_tag="ACHKIR_005505"
     CDS             6049..6582
                     /gene="blc"
                     /locus_tag="ACHKIR_005505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFO9993068.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6583..6900)
                     /gene="sugE"
                     /locus_tag="ACHKIR_005506"
     CDS             complement(6583..6900)
                     /gene="sugE"
                     /locus_tag="ACHKIR_005506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFO9993069.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7075..7221)
                     /gene="ecnB"
                     /locus_tag="ACHKIR_005507"
     CDS             complement(7075..7221)
                     /gene="ecnB"
                     /locus_tag="ACHKIR_005507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFO9993070.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
BASE COUNT         1842 a         1913 c         1908 g         1697 t
ORIGIN      
        1 ggcagcgtaa aaaccaatac agaattgata cctggatgat atcaaatatc cagtcttaaa
       61 ttgtggcctt tagcggaaaa atgaaaacat atgctacaga atggcgctta tcacgccatt
      121 cttcgcggtt aattcagtaa tttttcagaa ttatgcccgg tcaacgctaa aggcgatgac
      181 ttcagccagt gtctccgcgc ccagcgccaa catcaccaga cgatcaacgc ctaatgccac
      241 gccggaacag tcaggcatac cgactttcaa ggcttcaatc agattctggt caatggggtg
      301 ctgcggcaaa ccgcgcgccg cgcgcttacg gttatcttgt tcaaagcgtt gttgctgctc
      361 acgggcatcc gtcaattcat ggaaaccatt cgccagctca atacctttat aataaacctc
      421 aaagcgttca gcgacccgat gatcttcggt actgatttgc gccagtgatg cctggctggc
      481 tggaaagtgg tacacaaagg tcggtttttc tttgccaata tttggctcta cgccaaaggt
      541 aaacagcaat tgtagaagcg tgtcgcggtc ttcttcagta tcggcaacgt tgctcaaatc
      601 cagtttcgct gccacttccc gcagttgcgt tttgtcggca gagagtgggt caatttccag
      661 ataacgcaag aaagcttgtt gataagaaag gctttctgct gccggacagt ccagcacctg
      721 ttgcaagaga tcgtccacct cgttcatcaa ccggtacata tcatagtgcg gtcgatacca
      781 ctcaagcata gtgaactcag ggttgtgata acgccccatc tcttcattac ggaagctgcg
      841 gcacagctgg aataccggcc cacaaccagc aaccagcagg cgtttcatat ggtattccgg
      901 gctggtcatt aaccagagat tcatcccctg cgaatgcccg gggcctacga aacgtgtctc
      961 aaacgggacc aaatgaatat cggttaccgt cgcctggctc atacaaggcg tctccacctc
     1021 cagcactcca cgatcggcaa agaaacgacg gatctccgcc ataatcgccg cgcgttttaa
     1081 taagttagga atggatgcgc tcggctgcca ggatgccgtt tcgctcatag ttaaatctcc
     1141 agtttttgac aagggcacga agtctactcg caacgcgacg gcgagacaaa ttttacgcag
     1201 gaatcaaaca agggtgggca gtgactaaaa aaagcacgat ctgatggttt agtaattaaa
     1261 ttaatcatct tcagtgataa tttagccctc ttgcgcacta aaaaaatcga tctcgtcaaa
     1321 tttcagactt atccatcaga ctatactgtt gtacctataa aggagcagtg gaatagcgtt
     1381 cgcagaccgt aactttcagg cacttaccct gaagtacggg gctgtgggat aaaaacaatc
     1441 tggaggaatg tcgtgcaaac ctttcaagcc gatcttgcca ttgtaggcgc cggtggcgcg
     1501 ggattacgtg ctgcaattgc tgccgcgcag gcaaatccaa atgcaaaaat cgcactaatc
     1561 tcaaaagtat acccgatgcg tagccatacc gttgctgcag aagggggctc cgccgctgtc
     1621 gcgcaggatc atgacagctt cgaatatcac tttcacgata cagtagcggg tggcgactgg
     1681 ttgtgtgagc aggatgtcgt ggattatttc gtccaccact gccccaccga aatgacccaa
     1741 ctggaactgt ggggatgccc atggagccgt cgcccggatg gtagcgtcaa cgtacgtcgc
     1801 ttcggcggca tgaaaatcga gcgcacctgg ttcgccgccg ataagaccgg cttccatatg
     1861 ctgcacacgc tgttccagac ctctctgcaa ttcccgcaga tccagcgttt tgacgaacat
     1921 ttcgtactgg atattctggt tgatgatggt catgttcgcg gcctggtagc aatgaacatg
     1981 atggaaggca cgctggtgca gatccgtgct aacgcggtcg ttatggctac cggcggtgcg
     2041 ggtcgcgttt atcgttacaa taccaatggc ggcatcgtta ccggtgacgg tatgggtatg
     2101 gcgctaagcc acggcgttcc gctgcgtgac atggaattcg ttcagtatca cccaaccggt
     2161 ctgccaggtt ccggtatcct gatgaccgaa ggctgccgcg gtgaaggcgg tattctggtc
     2221 aacaaaaatg gctaccgtta tctgcaagat tacggcatgg gcccggaaac tccgctgggc
     2281 gagccgaaaa acaaatatat ggaactgggt ccacgcgaca aagtttctca ggccttctgg
     2341 cacgaatggc gtaaaggcaa cactatctcc acgccgcgtg gcgatgtggt ttatctcgac
     2401 ttgcgtcacc tcggcgagaa aaaactgcat gaacgtctgc cgttcatctg cgaactggcg
     2461 aaagcgtacg ttggcgtcga tccggttaaa gaaccgattc cggtacgtcc gaccgcacac
     2521 tacaccatgg gcggtatcga aaccgatcag aactgtgaaa cccgcattaa aggtctgttc
     2581 gccgtgggtg aatgttcctc tgttggtctg cacggtgcaa accgtctggg ttctaactcc
     2641 ctggcggaac tggtggtctt cggccgtctg gccggtgaac aagcgacaga gcgtgcagca
     2701 actgccggta atggcaacga agcggtaatt gaagcgcagg cagctggcgt tgaacaacgt
     2761 ttgaaagatc tggttaacca ggatggcggc gaaaactggg cgaagatccg cgacgaaatg
     2821 ggcctggcta tggaagaagg ctgcggtatc taccgtacgc cggaactgat gcagaaaacc
     2881 atcgacaagc tggcagagct gcaggaacgc ttcaaacgcg tgcgcatcac cgacacttcc
     2941 agcgtgttca acaccgacct gctctacacc attgaactgg gccacggtct gaacgttgct
     3001 gaatgtatgg cgcactccgc aatggcacgt aaagagtccc gcggcgcaca ccagcgtctg
     3061 gacgaaggtt gcaccgagcg tgacgacgtc aacttcctca aacacaccct cgccttccgc
     3121 gatgctgatg gcacgactcg cctggagtac agcgacgtga agattactac gctgccgcca
     3181 gctaaacgcg tttacggtgg cgaagcggat gcagccgata aggcggaagc agccaataag
     3241 aaggagaagg cgaatggctg agatgaaaaa cctgaaaatt gaggtggtgc gctataaccc
     3301 ggaagtcgat accgcaccgc atagcgcatt ctatgaagtg ccttatgacg caactacctc
     3361 attactggat gcgctgggct acatcaaaga caacctggca ccggacctga gctaccgctg
     3421 gtcctgccgt atggcgattt gtggttcctg cggcatgatg gttaacaacg tgccaaaact
     3481 ggcatgtaaa accttcctgc gtgattacac cgacggtatg aaggttgaag cgttagctaa
     3541 cttcccgatt gaacgcgatc tggtggtcga tatgacccac ttcatcgaaa gtctggaagc
     3601 gatcaaaccg tacatcatcg gcaactcccg caccgcggat cagggtacta acatccagac
     3661 cccggcgcag atggccaagt atcaccagtt ctccggttgc atcaactgtg gtctgtgcta
     3721 cgccgcgtgc ccgcagtttg gcctgaaccc agagttcatc ggtccggctg ccattacgct
     3781 ggcgcatcgt tataacgaag atagccgcga ccacggtaag aaggagcgta tggcgcagtt
     3841 gaacagccag aacggcgtat ggagctgtac tttcgtgggc tactgctccg aagtctgccc
     3901 gaaacacgtc gatccggctg cggccattca gcagggcaaa gtagaaagtt cgaaagactt
     3961 tcttatcgcg accctgaaac cacgctaagg agtgcaacat gacgactaaa cgtaaaccgt
     4021 atgtacggcc aatgacgtcc acctggtgga aaaaattgcc gttttatcgc ttctacatgc
     4081 tgcgcgaagg cacggcggtt ccggctgtgt ggttcagcat tgaactgatt ttcgggctgt
     4141 ttgccctgaa aaatggcccg gaagcctggg cgggattcgt cgacttttta caaaacccgg
     4201 ttatcgtgat cattaacctg atcactctgg cggcagccct gctgcacacc aaaacctggt
     4261 ttgagctggc accaaaagcg gccaatatca ttgtaaaaga cgaaaaaatg ggaccagagc
     4321 caattatcaa aagcctctgg gcggtaactg tggttgccac catcgtaatc ctgtttgttg
     4381 ccctgtactg gtaaggagcc tgagatgatt aatccaaatc caaagcgttc tgacgaaccg
     4441 gtattctggg gcctcttcgg ggccggtggt atgtggagcg caattattgc gccggtgatg
     4501 atcctgctgg ttggtattct gctgccactg gggctgtttc cgggtgacgc actgagctac
     4561 gagcgcgttc tggcgttcgc gcagagcttc attggtcgcg tattcctgtt cctgatgatc
     4621 gttctgccgc tgtggtgtgg tttacaccgt atgcaccacg cgatgcacga tctgaaaatc
     4681 cacgtacctg cgggcaaatg ggttttctac ggtctggctg ctatcctgac agttgtcacg
     4741 ctgattggtg tcgttacaat ctaacgcatc gccaatgtaa atccggcccg cctatggcgg
     4801 gccgttttgt atggaaacca gaccctatgt tcaaaatgac gctctgcgcc ttattaatta
     4861 ccgcctcttg ctccacattt gctgcccctc aacaaatcaa cgatattgtg catcgcacaa
     4921 ttaccccgct tatagagcaa caaaagatcc ccggtatagc ggtggcggta atttatcagg
     4981 gtaaacctta ttactttacc tggggctatg cggacatcgc caaaaagcag cccgtcacac
     5041 agcaaacgtt gtttgagtta ggttcggtca gcaaaacatt tacgggcgtg cttggtggcg
     5101 acgctattgc tcgaggggaa atcaagttaa gcgatcccgc gacaaaatac tggcctgaac
     5161 ttaccgctaa acaatggaat gggatcacac tattacatct cgcgacctac accgctggcg
     5221 gcctgccatt gcaggtgccg gatgacgtta aatcctcaag cgacttgctg cgcttctatc
     5281 aaaactggca acctgcatgg gctccaggaa cacaacgtct gtatgccaac gccagtattg
     5341 ggttgttcgg tgcactggcg gtgaaaccat ccggtttgag ctttgaacag gcgatgcaaa
     5401 ctcgtgtttt ccagccactc aaactcactc atacgtggat taatgtgccg tccgcagaag
     5461 aaaagaatta cgcctgggga tatcgcgaag gtaaggcagt gcatgtttcg ccaggggcgt
     5521 tagatgctga ggcttatggt gtgaagtcga ccattgaaga tatggcccgc tgggtgcaaa
     5581 gcaatttaaa accccttgat atcactgaga aaacgcttca acaagggata caactggcac
     5641 aatctcgcta ctggcaaacc ggcgatatgt atcagggcct gggctgggaa atgctggact
     5701 ggccggtcaa tcctgacatc atcattaacg gcagtgacaa taaaattgca ctggcagcac
     5761 gccccgtaaa agcgattacg cccccaactc ctgcagtacg cgcatcatgg gtacataaaa
     5821 cgggggcgac cggcggattt ggtagctatg tcgcgtttat tccagaaaaa gagctgggta
     5881 tcgtgatgct ggctaacaaa aactatccca atccagcgag agtcgccgcc gcctgtcaga
     5941 ttctcaacgc tctacagtaa aatttcatcg ggtccgaatt tttggacctt ttctccgctt
     6001 ttccttgctg tcatctacac ttagaaaaaa accagtaagg aaacatttat gcgcctgctc
     6061 cctctcgttg ccgcagcgac agctgcattt ctggtcgttg cctgcagttc tcctacgccg
     6121 ccccgtggcg tgaccgtagt gaataatttc gatgctaaac gctatctcgg tacctggtat
     6181 gagattgccc gttttgatca ccgttttgaa cgtggactgg aaaaagtcac cgcaacatac
     6241 agcctgcgtg atgacggcgg cctgaatgtc attaataaag gctataaccc tgacagagaa
     6301 atgtggcagc agagtgaagg gaaagcgtac tttaccggcg caccaactcg cgctgcgctg
     6361 aaagtgtcat tctttggtcc tttctatggc ggttataacg ttattgcact cgatcgggaa
     6421 taccgccatg cgctggtttg cggtccggac cgcgactacc tgtggatact ctcccgcacg
     6481 ccaaccattt ctgacgaagt gaaacaggag atgctggcag tcgcgacccg ggaagggttt
     6541 gatgtcagta agtttatttg ggtacagcag cccggtagtt agttagtgag tgctgagttt
     6601 cagaccaata atccccaata cgattaacgc cagactcgcc aggcgcatcg ggttagcgga
     6661 ctcgccgagc agcacaatgc cggtgatggc cgcgccgact gcgccaatac ccgtccacac
     6721 ggcataagcc gtccctactg gtaacgattt catcgcccag gcaagtagcg ccatactgac
     6781 aatcatcgcc gtcacagtaa taacacttgg cgtcaaacga ctaaagccgt gggtatattt
     6841 caggccaacg gcccatacca cttccagcag gccagcaata actaagataa tccaggacat
     6901 atcaggctcc ggagaattgg ggccgtcccc ggtgaaaaga ggcgtttgca ggtcgtcctg
     6961 caaagctgat ggttgaacgg atattttgcc aggtggcagg ggaatttcaa ctattacctt
     7021 tgtgactatc gcaaaaaaac aaacggcacg acacagctgt cgtaccgtta ttgcttactg
     7081 ctgcgctttc gttgctgcgc cagaaatcgc gttaccgcca tcagaaatgt cttcaccaac
     7141 gccacgcgtg gtgttgcaag cagttaatac tgttgaaagc accagaacag aaaagatcgc
     7201 tgcaattgtc ttcttcacca taacgtcttc cttttaggta atgttattta tgtttgccta
     7261 tagcaaatta ataatagaca acatcgccaa aaatgatgga aatctgatga tttttcggag
     7321 gagaagaaaa attagctggc agcgcgggag atggagttgc
//
LOCUS       DAZGQL010000442         5446 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.guided.189, whole genome shotgun sequence.
ACCESSION   DAZGQL010000442 DAZGQL010000000
VERSION     DAZGQL010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5446
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.guided.189"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            120..326
                     /locus_tag="ACHKIR_005508"
                     /pseudo
     CDS             120..326
                     /locus_tag="ACHKIR_005508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
     gene            322..674
                     /gene="prfH"
                     /locus_tag="ACHKIR_005509"
                     /pseudo
     CDS             322..674
                     /gene="prfH"
                     /locus_tag="ACHKIR_005509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="similar to release factor 1 and 2; frameshifted;
                     incomplete; partial in the middle of a contig; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(731..2188)
                     /gene="pepD"
                     /locus_tag="ACHKIR_005510"
     CDS             complement(731..2188)
                     /gene="pepD"
                     /locus_tag="ACHKIR_005510"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFO9993071.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            2449..2907
                     /gene="gpt"
                     /locus_tag="ACHKIR_005511"
     CDS             2449..2907
                     /gene="gpt"
                     /locus_tag="ACHKIR_005511"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFO9993072.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQNTWIEQPWDMGVVFVPPISGR"
     gene            2999..4243
                     /gene="frsA"
                     /locus_tag="ACHKIR_005512"
     CDS             2999..4243
                     /gene="frsA"
                     /locus_tag="ACHKIR_005512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFO9993073.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFSHGAQLPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRYWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKVVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITDWIEKRLC"
     gene            4301..4702
                     /gene="crl"
                     /locus_tag="ACHKIR_005513"
     CDS             4301..4702
                     /gene="crl"
                     /locus_tag="ACHKIR_005513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFO9993074.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWTSVQIKDAEVVERLEHTLRE
                     FHEKLRELLATLNLKLEPADDFRDEPVKLTA"
     gene            complement(4741..>5446)
                     /locus_tag="ACHKIR_005514"
     CDS             complement(4741..>5446)
                     /locus_tag="ACHKIR_005514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFO9993075.1"
                     /translation="ALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFFG
                     VIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQNL
                     QSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQFD
                     FGLRPSLGYVLSKGKDIEGIGDEDLVNYVDVGATYYFNKNMSAFVDYKINQLESDNKL
                     NINNDDIVAVGMTYQF"
BASE COUNT         1322 a         1336 c         1411 g         1377 t
ORIGIN      
        1 tgcaggaagg cgacaagcga gtgaatcccc aggagcttac taaagtaagt gactggggtg
       61 aacgaacgcg gtcgcagtac atgcaacttg aagtatgacg agtataaatg gaatgataca
      121 tgggcaaata tattcgtccc ttatccgatg cggtatttac catcgcatct gatgacctgt
      181 ggatcgagag tttagcgatc caacaattac acaccacggc aaatttaccc aacatgcagc
      241 gcgttgtggg gatgccagat ttacatcccg gacgcggcta cccgattggc gcagcgttct
      301 tctctgttgg tcgtttttac ccggcaagac gtcgcggtaa cggtgctgga aacagaaacg
      361 ggccgctact ctgacacgct gcgttcggcg ctgatttctc tggatggcga taatgcatgg
      421 gcattaagcg aaagttggtg cggcactatt cagtgtattt gtccgagtcc gtatcggcct
      481 catcatgggc gcaaaaactg gtttctgggc atgctaacaa gcggctggcg cgattgctga
      541 ttgcctggaa gctggaacaa cagcaacagg aaaatagcgc ggcgctgaaa tcgcagcggc
      601 gaatgttcca tcaccagatt gaacgtggca acccgcgacg aacgttcaca gggatggctt
      661 ttatcgaagg ataatgaagg atgaaactgc cggaaggcga ttaaacgcca tccggcagca
      721 aatcaaataa ttacttcgcc ggaatttctt tcagcagttc agtcagtagt gtccagtaat
      781 gacctacgct ttcgatgtga acttgctcat ccggagagtg tggaccggtg atggttggcc
      841 cgatagaaac catgtccatt tccggatacg gttttttgaa cagaccacat tccaggcccg
      901 cgtggataat ctggatgttc ggcgtcttgt tgaacaggcg ctgataggtt tcacgtacca
      961 gatgcatcac cggagaatta gcgtccggct gccagccagg atatgcgcct ttcgcttcgg
     1021 ttttcgcgcc agccagttta cccagcgaat ccagcatgct caccacgtag tctttaccgc
     1081 tgtcgatcag agaacggatc aggcagtgaa tttctacgtt attgtcagtc atggtcacca
     1141 caccgacgtt cagggaggtt tcgaccacac cttttgccac gtcggagttg cggatcacac
     1201 cgttcggggt ggcgttcagc agacgaataa aggtatcgcg agatttcgca atcagggcag
     1261 ctttatcgtt cgcaacagag tccagcaaca aggccagatt tttctctttc tctgccagct
     1321 cgtttttcag gatctcctga taggtattca ccagagattt caaagcgtcg actttatcag
     1381 ctgcgacagc aatggtcgca aaggcttcac gcgggatggc gttacgcagt gtgccgccgt
     1441 tgaaatcgat aaggcgcagg tccagttctt ctgcatgacc cgccaggaag cgcaccagca
     1501 gtttgttggc attacccagg ccaacgtgga tttcaccgcc ggagtgaccg cctttcaggc
     1561 cttttaaggt taacttgaag gtttcaaagc cagctggaac cgcttcacga tctaaatgca
     1621 ggttggaggt gaagtcgata cctcccgcac aacccatgta gatttcacct tcttcttcgg
     1681 agtcggtgtt aatcagaata tcagcctgca accagttgct ctgtaagcca aaagcaccgt
     1741 ccataccggc ttcttcggtc atggtcagca gcacttccag cgggccgtga accacgtttt
     1801 cgtcagccag aaccgccagt gcagaggcca taccaatgcc gttatccgca cccagcgtgg
     1861 tgccgcgtgc tttaacccat tcgccatcaa tataaggctg gataggatct ttggtgaagt
     1921 catgcacggt gtcgttattt ttttgcggca ccatatcgag gtgggcctgc aagacgaccg
     1981 gtttacgatt ttccatacct gcggtagcag gtttacgaat caggatatta cctacctgat
     2041 cgcgttcgac atggaaacct ttctcttttg cccaaccaac aatgtattca gcgagttgct
     2101 cttcatgata ggacgggtga ggaatagaac agattttggc aaaaatatcc cacagcggct
     2161 gtggagataa ttgagacagt tcagacacgt taagtctcct tgtcgatcac ccgcaaaaca
     2221 gtattgcagg tcacagggtt agcagaaaat gttgtcaaca caagacaggc ttgcgagata
     2281 tgtttgagaa taccacttta tcccgcgtca gggagaggca gtgcgtaaaa agacgcggac
     2341 tcatgtgaaa tactggtttt tagtgcgcca gatctctata atctcgcgca acctattttc
     2401 ccctcgaaca ctttttaagc cgtagataaa caggctggga cacttcacat gagcgaaaaa
     2461 tacatcgtca cctgggacat gttgcagatc catgcacgta aactcgcaag ccgactgatg
     2521 ccttctgaac aatggaaagg cattattgcc gtaagccgtg gcggtctggt accgggtgcg
     2581 ttactggcgc gtgaactggg tattcgtcat gtcgataccg tttgtatttc cagctacgat
     2641 cacgacaacc agcgcgagct taaagtgctg aaacgcgcag aaggcgatgg cgaaggcttc
     2701 atcgttattg atgacctggt agataccggt ggtactgcgg ttgcgattcg tgaaatgtat
     2761 ccaaaagcgc actttgtcac catcttcgca aaaccggctg gtcgtccgct ggttgatgat
     2821 tatgttgttg atatcccgca aaatacctgg atcgaacagc cgtgggatat gggcgtcgta
     2881 ttcgtgccgc caatctccgg tcgctaatct tttcaacgcc tggcactgcc gggcgttgtt
     2941 ctttttaact tcaggcgggt tacaatagtt tccagtaagt attctggagg ctgcatccat
     3001 gacacaggca aacctgagcg aaaccctgtt caaaccccgc tttaaacatc ctgaaacctc
     3061 gacgctagtc cgccgcttta gtcacggcgc acaactgcct gtgcagtcgg cccttgatgg
     3121 taaaaccatc cctcactggt atcgcatgat taaccgtctg atgtggatct ggcgcggcat
     3181 tgacccccgt gaaatcctcg acgtccaggc gcgtattgtg atgagcgatg ccgaacgtac
     3241 cgacgatgat ttatacgata cggtgattgg ctaccgcggc ggcaactgga tttatgagtg
     3301 ggctacccag gcgatggtgt ggcaacaaaa agcctgtgcg gaagaagatc cgcaactcag
     3361 cggtcgatac tggctgcatg cggctacgtt gtacaacatt gctgcctatc ctcatctgaa
     3421 aggagatgac ctggccgagc aagcgcaggc tttgtcaaac cgcgcctatg aagaggccgc
     3481 tcagcgtctg ccgggcacga tgcggcagat ggagtttacc gtacccggcg gtgcgcccat
     3541 caccggcttt ttgcatatgc cgaaaggcga tggcccgttc ccgacagtat taatgtgtgg
     3601 tggtctggat gcgatgcaga cggactatta cagcctgtat gaacgttatt ttgcgccgcg
     3661 cggcattgct atgctgacta ttgatatgcc gtcggtgggc ttttcttcaa aatggaagct
     3721 cacccaggac tccagcctgt tgcatcagca cgttttaaag gcgctgccta acgtaccgtg
     3781 ggtggatcac actcgcgtcg cggcctttgg tttccgtttc ggcgctaacg ttgccgtgcg
     3841 tctggcatac cttgaatcgc cgcgtctgaa agtggttgcc tgtcttggtc cggtagttca
     3901 taccctgttg agtgatttta agtgccagca acaggtgccg gaaatgtatc ttgacgttct
     3961 ggccagtcgt ttagggatgc atgatgcttc cgatgaagcg ttgcgagtgg agttgaatcg
     4021 ctattcatta aaagtgcaag gattgctggg acgtcgctgc ccaacgccaa tgttatcagg
     4081 ctactggaag aacgatccgt tcagcccgga agaggactca cgcttaatca cgtcgtcatc
     4141 tgctgacggt aaattattag agatcccatt taacccggtg tatcggaatt ttgacaaagg
     4201 ccttcaggaa atcacagact ggatcgaaaa acgtttgtgt taaaaatttg ctaaatttta
     4261 ccaatttggt aaaacagttg catcacaaca ggagattgca atgacgttac cgagtggaca
     4321 cccgaagagc agattgatca aaaaatttac cgcactaggc ccgtatattc gtgaaggtaa
     4381 gtgcgaagat aatcgattct ttttcgattg tctggctgta tgcgtcaacg tgaaaccggc
     4441 accggaagtg cgtgagttct ggggctggtg gatggaactg gaagcgcagg aatcccgttt
     4501 tacctacagt taccagtttg gtctgttcga taaagcaggc gactggacga gtgttcagat
     4561 aaaagatgcc gaagtggttg aacgactgga gcacaccctg cgtgaatttc acgagaagct
     4621 gcgtgagctg ctggcgacgc tgaatctgaa gctggaaccg gcagatgatt ttcgtgatga
     4681 accggtgaag ttaacggcgt gaatgaaatg tgccggatgc atcacatccg gcaatattca
     4741 ttaaaactga tacgtcatcc caaccgcgac aatatcatca ttattaatat ttaatttgtt
     4801 atcgctttcc agttggttga ttttataatc aacaaacgct gacatatttt tgttgaaata
     4861 atacgtagca ccaacatcga cataattgac cagatcttca tcaccgatgc cctcaatatc
     4921 tttccctttc gataagacat aacccagcga tggacgcaga ccaaagtcaa actggtattg
     4981 agcgaccgct tcaaagttct gcgtcttatt ggcaaagccg ccagttattg gcgtcatttt
     5041 gcgtgtttca gaatagaaag ttgccagata aatattattg gcatcgtatt tcagacctgt
     5101 tgcccatgct tctgcacgct tgcctgtgcc acggctttgc aggttctgct cgttggtgcg
     5161 atcagagttg gtataggctc cactgatggc gaaatcgctg ccaccaaagt catatgtcaa
     5221 tgacgtgccg aagccatcgc cgttttgctt tttaacgtcg cggttttcgt ttttcccttg
     5281 atattgcagg gttaagttca ggccatcgat aacgccgaag aagtcggtgt tccgatacgt
     5341 cgccagaccg ctggcgcgtt tggtcataaa gttgtcggtc tgcgccgagg agtcgccacc
     5401 aaattccggg aacatatcgg tccaggcttc cacgtcatac aacgcg
//
LOCUS       DAZGQL010000443         1630 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.guided.279, whole genome shotgun sequence.
ACCESSION   DAZGQL010000443 DAZGQL010000000
VERSION     DAZGQL010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.guided.279"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            259..1206
                     /gene="stxA1a"
                     /locus_tag="ACHKIR_005515"
     CDS             259..1206
                     /gene="stxA1a"
                     /locus_tag="ACHKIR_005515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1a subunit A"
                     /protein_id="HFO9993076.1"
                     /translation="MKIIIFRVLTFFFVIFSVNVVAKEFTLDFSTAKTYVDSLNVIRS
                     AIGTPLQTISSGGTSLLMIDSGTGDNLFAVDVRGIDPEEGRFNNLRLIVERNNLYVTG
                     FVNRTNNVFYRFADFSHVTFPGTTAVTLSGDSSYTTLQRVAGISRTGMQINRHSLTTS
                     YLDLMSHSGTSLTQSVARAMLRFVTVTAEALRFRQIQRGFRTTLDDLSGRSYVMTAED
                     VDLTLNWGRLSSVLPDYHGQDSVRVGRISFGSINAILGSVALILNCHHHASRVARMAS
                     DEFPSMCPADGRVRGITHNKILWDSSTLGAILMRRTISS"
     gene            1216..1485
                     /gene="stxB1a"
                     /locus_tag="ACHKIR_005516"
     CDS             1216..1485
                     /gene="stxB1a"
                     /locus_tag="ACHKIR_005516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1a subunit B"
                     /protein_id="HFO9993077.1"
                     /translation="MKKTLLIAASLSFFSASALATPDCVTGKVEYTKYNDDDTFTVKV
                     GDKELFTNRWNLQSLLLSAQITGMTVTIKTNACHNGGGFSEVIFR"
BASE COUNT          437 a          278 c          394 g          521 t
ORIGIN      
        1 ttgccagcct cccccagtgg ctggcttttt tatgtccgta acatcctgtg tatcaataaa
       61 tgttgttgtc tacgtacgtc aagtagtcgc atgagatctg accagatatg ttaaggttgc
      121 agctctcttt gaatataatt atcattttca ttacgttatt gttacgttta tccggtgcgc
      181 cgtaaaacgc cgtccttcag ggggtggagg atgtcaagaa tatagttatc gtatggtgct
      241 caaggagtat tgtgtaatat gaaaataatt atttttagag tgctaacttt tttctttgtt
      301 atcttttcag ttaatgtggt tgcgaaggaa tttaccttag acttctcgac tgcaaagacg
      361 tatgtagatt cgctgaatgt cattcgctct gcaataggta ctccattaca gactatttca
      421 tcaggaggta cgtctttact gatgattgat agtggcacag gggataattt gtttgcagtt
      481 gatgtcagag ggatagatcc agaggaaggg cggtttaata atctacggct tattgttgaa
      541 cgaaataatt tatatgtgac aggatttgtt aacaggacaa ataatgtttt ttatcgcttt
      601 gctgattttt cacatgttac ctttccaggt acaacagcgg ttacattgtc tggtgacagt
      661 agctatacca cgttacagcg tgttgcaggg atcagtcgta cggggatgca gataaatcgc
      721 cattcgttga ctacttctta tctggattta atgtcgcata gtggaacctc actgacgcag
      781 tctgtggcaa gagcgatgtt acggtttgtt actgtgacag ctgaagcttt acgttttcgg
      841 caaatacaga ggggatttcg tacaacactg gatgatctca gtgggcgttc ttatgtaatg
      901 actgctgaag atgttgatct tacattgaac tggggaaggt tgagtagtgt cctgcctgac
      961 tatcatggac aagactctgt tcgtgtagga agaatttctt ttggaagcat taatgcaatt
     1021 ctgggaagcg tggcattaat actgaattgt catcatcatg catcgcgagt tgccagaatg
     1081 gcatctgatg agtttccttc tatgtgtccg gcagatggaa gagtccgtgg gattacgcac
     1141 aataaaatat tgtgggattc atccactctg ggggcaattc tgatgcgcag aactattagc
     1201 agttgagggg gtaaaatgaa aaaaacatta ttaatagctg catcgctttc atttttttca
     1261 gcaagtgcgc tggcgacgcc tgattgtgta actggaaagg tggagtatac aaaatataat
     1321 gatgacgata cctttacagt taaagtgggt gataaagaat tatttaccaa cagatggaat
     1381 cttcagtctc ttcttctcag tgcgcaaatt acggggatga ctgtaaccat taaaactaat
     1441 gcctgtcata atggaggggg attcagcgaa gttatttttc gttgactcag aatagctcag
     1501 tgaaaatagc aggcggagat tcataaatgt taaatacatc tcaattcagt cagttgttgc
     1561 ctgtctgata atagatgtgt tagaaaattt ctgcatggtg aatccccctg tgcggagggg
     1621 cgactggtga
//
LOCUS       DAZGQL010000444         1465 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2112H21385
            SAMN43267765-rid23071233.guided.293, whole genome shotgun sequence.
ACCESSION   DAZGQL010000444 DAZGQL010000000
VERSION     DAZGQL010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267765
            Sequence Read Archive: SRR30307531
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350849.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350849.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,518
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,263
            CDSs (with protein)               :: 5,263
            Genes (RNA)                       :: 87
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 67 of 168
            Pseudo Genes (incomplete)         :: 121 of 168
            Pseudo Genes (internal stop)      :: 41 of 168
            Pseudo Genes (multiple problems)  :: 50 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1465
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267765-rid23071233.guided.293"
                     /serotype="O146:H28"
                     /isolate="2112H21385"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-12"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            132..1091
                     /gene="stxA2b"
                     /locus_tag="ACHKIR_005517"
     CDS             132..1091
                     /gene="stxA2b"
                     /locus_tag="ACHKIR_005517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2b subunit A"
                     /protein_id="HFO9993078.1"
                     /translation="MKCILLKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFAHISVPGVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNAMTRDASRAVLRFVTVTAEALRFRQIQREFRLALSETAPVYTMTPEEV
                     DLTLNWGRISNVLPEFRGEGGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNE
                     EIQPECQITGDRPVIRINNTLWESNTAAAFLNRRAHSLNTSGE"
     gene            1104..1367
                     /gene="stxB2b"
                     /locus_tag="ACHKIR_005518"
     CDS             1104..1367
                     /gene="stxB2b"
                     /locus_tag="ACHKIR_005518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2b subunit B"
                     /protein_id="HFO9993079.1"
                     /translation="MKKIFVAALFAFVSVNAMAADCAKGKIEFSKYNENDTFTVKVAG
                     KEYWTNRWNLQPLLQSAQLTGMTVTIKSNTCASGSGFAEVQFN"
BASE COUNT          401 a          296 c          353 g          415 t
ORIGIN      
        1 aagcaatcgg tcactggttc gaatccagta caacgcgcca tacttatttt ttctggctcg
       61 cttttgcggg ccttttttat atctgcgccg ggtctggtgc tgattacttc agccaaaagg
      121 aatacctgta tatgaagtgt atattgttaa aatgggtact gtgcctgtta ctgggctttt
      181 cttcggtatc ctattcccgg gaatttacga tagacttttc gacccaacaa agttatgtct
      241 cttcgttaaa tagtatacgg acagaaatat cgaccccact tgaacatata tctcagggga
      301 ccacatcggt gtctgttatt aaccacaccc caccgggcag ttattttgct gtggatatac
      361 gagggcttga tgtctatcag gcgcgttttg accatcttcg tctgattatt gagcaaaata
      421 atttatatgt ggccggattc gttaatacgg caacaaatac tttctacagg ttttcagatt
      481 ttgcacatat atcagtgccc ggtgtgacaa ctgtttccat gacaacggac agcagttata
      541 ccactctgca gcgtgtcgca gcgctggaac gttccggaat gcaaatcagt cgtcactcac
      601 tggtttcatc atatctggcg ttaatggagt ttagtggaaa tgccatgacc agagatgcat
      661 ccagagcagt tctgcgtttt gtcactgtca cagcagaagc cttacggttc aggcaaatac
      721 agagagaatt tcgtctggca ctgtctgaaa ctgctcctgt ttatacgatg acaccggaag
      781 aagtggacct cacactgaac tgggggagaa tcagcaatgt gcttccggag tttcggggag
      841 agggtggtgt cagagtgggg cgaatatcct ttaataatat atcagcgata ctgggcacag
      901 tggcggttat actgaattgc catcatcagg gggcgcgttc cgttcgcgcc gtgaatgaag
      961 agatacaacc agaatgtcag ataactggcg acaggccagt tataaggata aacaatacgt
     1021 tatgggaaag taataccgca gctgcttttc tgaatcgcag ggctcactct ttaaatacat
     1081 ccggagaata acaggagtta aatatgaaga agatatttgt agcggcttta tttgcttttg
     1141 tttctgttaa tgcaatggca gctgattgtg caaaaggtaa aattgagttc tctaagtata
     1201 atgagaatga tacattcaca gtaaaagtgg ccgggaaaga gtactggact aaccgctgga
     1261 atctgcaacc gctactgcaa agcgcacagt taacaggaat gacggtaaca atcaaatcaa
     1321 atacctgtgc gtcaggttca ggatttgctg aagtgcagtt taattaatat cagaagcatt
     1381 gctggtttcg tggtgtgcag caatgtagtt acagtgtaat caatgtcaca attcagtcag
     1441 ttgaaggttg tctgcccgac tgaga
//
